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Sample records for evaluation procedures hep

  1. Habitat Evaluation Procedures (HEP) Report : Ladd Marsh, 2001 Technical Report.

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    Oregon Department of Fish and Wildlife

    2001-10-01

    Since the mid-1980s, the Oregon Department of Fish and Wildlife (ODFW) has been participating in the Bonneville Power Administration's (BPA's) efforts to mitigate for the negative impacts to fish and wildlife resulting from the development and operation of the 7 Columbia Basin Federal Hydropower System. BPA's mitigation obligations were formally recognized and mandated by the Northwest Power Act of 1980 and are guided by the Northwest Power Planning Council's (NWPPC's) Columbia Basin Fish and Wildlife Program. BPA funds fish and wildlife projects throughout the Basin to meet the habitat and population restorative goals and objectives outlined in the NWPPC's Fish and Wildlife Program and to fulfill its mitigation responsibilities under the Power Act. Impacts to wildlife resulting from hydrofacility construction/inundation were estimated using Habitat Evaluation Procedures (HEP) in the mid and late 1980s and are documented in BPA' s Wildlife Loss Assessments (Rasmussen and Wright 1990,a,b,c,d) and in the U.S. Army Corps of Engineers Lower Snake River Wildlife Habitat Compensation Evaluation (ACOE 1991). The loss assessments provided estimates of lost habitat quality and quantity for the target species selected to represent the habitat cover types impacted by hydropower construction/inundation. The NWPPC incorporated these losses into their Fish and Wildlife Program, recognizing them as the unannualized losses attributable to the construction/inundation of the federal hydropower system (NWPPC 1995 and 2000, Table 1 1-4). The HEP methodology is used by wildlife managers within the Columbia Basin to determine habitat values, expressed as Habitat Units, gained through BPA-funded mitigation project work. ODFW and the other Oregon wildlife managers (i.e., U.S. Fish and Wildlife Service, Confederated Tribes of the Warms Springs Reservation of Oregon, Burns Paiute Tribe, and Confederated Tribes of the Umatilla Indian Reservation [CTUIR

  2. Habitat Evaluation Procedures (HEP) Report : Grand Coulee Dam Mitigation, 1996-1999 Technical Report.

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    Kieffer, B.; Singer, Kelly; Abrahamson, Twa-le

    1999-07-01

    The purpose of this Habitat Evaluation Procedures (HEP) study was to determine baseline habitat units and to estimate future habitat units for Bonneville Power Administration (BPA) mitigation projects on the Spokane Indian Reservation. The mitigation between BPA and the Spokane Tribe of Indians (STOI) is for wildlife habitat losses on account of the construction of Grand Coulee Dam. Analysis of the HEP survey data will assist in mitigation crediting and appropriate management of the mitigation lands.

  3. Habitat Evaluation Procedures (HEP) Report Wanaket Wildlife Area, Techical Report 2005-2006.

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    Ashley, Paul

    2006-02-01

    The Regional HEP Team (RHT) and Confederated Tribes of the Umatilla Indian Reservation (CTUIR) Wildlife Program staff conducted a follow-up habitat evaluation procedures (HEP) analysis on the Wanaket Wildlife Management Area in June 2005. The 2005 HEP investigation generated 3,084.48 habitat units (HUs) for a net increase of 752.18 HUs above 1990/1995 baseline survey results. The HU to acre ratio also increased from 0.84:1.0 to 1.16:1.0. The largest increase in habitat units occurred in the shrubsteppe/grassland cover type (California quail and western meadowlark models), which increased from 1,544 HUs to 2,777 HUs (+43%), while agriculture cover type HUs were eliminated because agricultural lands (managed pasture) were converted to shrubsteppe/grassland. In addition to the agriculture cover type, major changes in habitat structure occurred in the shrubsteppe/grassland cover type due to the 2001 wildfire which removed the shrub component from well over 95% of its former range. The number of acres of all other cover types remained relatively stable; however, habitat quality improved in the riparian herb and riparian shrub cover types. The number and type of HEP species models used during the 2005 HEP analysis were identical to those used in the 1990/1995 baseline HEP surveys. The number of species models employed to evaluate the shrubsteppe/grassland, sand/gravel/mud/cobble, and riparian herb cover types, however, were fewer than reported in the McNary Dam Loss Assessment (Rassmussen and Wright 1989) for the same cover types.

  4. West Foster Creek 2007 Follow-up Habitat Evaluation Procedures (HEP) Report.

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    Ashley, Paul R.

    2008-02-01

    A follow-up habitat evaluation procedures (HEP) analysis was conducted on the West Foster Creek (Smith acquisition) wildlife mitigation site in May 2007 to determine the number of additional habitat units to credit Bonneville Power Administration (BPA) for providing funds to enhance and maintain the project site as partial mitigation for habitat losses associated with construction of Grand Coulee Dam. The West Foster Creek 2007 follow-up HEP survey generated 2,981.96 habitat units (HU) or 1.51 HUs per acre for a 34% increase (+751.34 HUs) above baseline HU credit (the 1999 baseline HEP survey generated 2,230.62 habitat units or 1.13 HUs per acre). The 2007 follow-up HEP analysis yielded 1,380.26 sharp-tailed grouse (Tympanuchus phasianellus) habitat units, 879.40 mule deer (Odocoileus hemionus) HUs, and 722.29 western meadowlark (Sturnella neglecta) habitat units. Mule deer and sharp-tailed grouse habitat units increased by 346.42 HUs and 470.62 HUs respectively over baseline (1999) survey results due largely to cessation of livestock grazing and subsequent passive restoration. In contrast, the western meadowlark generated slightly fewer habitat units in 2007 (-67.31) than in 1999, because of increased shrub cover, which lowers habitat suitability for that species.

  5. Habitat Evaluation Procedures (HEP) Report; Yakama Nation Wildlife Management Areas, Technical Report 1999-2000.

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    Raedeke, Kenneth; Raedeke, Dorothy

    2000-06-01

    Construction of the Dalles, Bonneville, McNary, and John Day Dams on the Columbia River by the federal government resulted in a substantial loss of riparian bottomland along the Columbia River. Impacts associated with the Mid-Columbia Projects were assessed for several wildlife species using the Habitat Evaluation Procedures (HEP) developed by the U.S. Fish and Wildlife Service (USDI-FWS 1980). The studies documented the loss of riparian habitat and established a baseline against which mitigation measures could be developed (USDI-FWS 1990 and USDE-BPA 1990). The impact assessments established a mitigation goal, a portion of which would be satisfied by the creation, restoration, and enhancement of riparian lands on tributaries to the Columbia River, including the Yakima Valley. The Yakama Nation (YN), the Northwest Power Planning Council, and the Bonneville Power Administration have agreed that the Yakama Nation would be funded to implement habitat restoration on lands within and adjacent to their reservation. Some of the targeted lands are owned by the Yakama Nation, some are trust lands, and some lands have been in private ownership. Since the early 1990s, the Yakama Nation has been in the process of assembling riparian lands into Wildlife Management Areas, and restoring natural hydrology and natural cover-types on these lands. The Northwest Power Planning Council, through the Bonneville Power Administration, has supported the program. HEP studies were performed by the Yakama Nation in 1990 (Bich et al. 1991) to establish baseline conditions and inventory wildlife habitat at the initiation of the restoration project. The 1990 HEP used a simplified version of the HEP to quantify baseline conditions. The present assessment is designed to evaluate the progress of the mitigation plan in meeting its stated goals. The 1999 HEP assessment has two distinct tasks: (1) Evaluation of the mitigation plan as currently implemented using the simplified YN HEP methodologies for

  6. Habitat Evaluation Procedures (HEP) Report : Ladd Marsh Wildlife Area, 2004-2006 Technical Report.

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    Ashley, Paul; Wagoner, Sara

    2006-05-01

    The Regional HEP Team (RHT) and Oregon Department of Fish and Wildlife (ODFW) staff conducted a follow-up habitat evaluation procedures (HEP) analysis on the Ladd Marsh Wildlife Management Area (LMWA) in May 2005. The 2005 HEP assessment resulted in a total of 647.44 HUs, or 0.76 HUs/acre. This is an increase of 420.34 HUs (0.49 HUs/acre) over 2001 HEP survey results. The most significant increase in HUs occurred on the Wallender and Simonis parcels which increased by 214.30 HUs and 177.49 HUs respectively. Transects were established at or near 2001 HEP analysis transect locations whenever possible. ODFW staff biologists assisted the RHT re-establish transect locations and/or suggested areas for new surveys. Since 2001, significant changes in cover type acreage and/or structural conditions have occurred due to conversion of agriculture cover types to emergent wetland and grassland cover types. Agricultural lands were seeded to reestablish grasslands and wetlands were restored through active management and manipulation of extant water sources including natural stream hydrology/flood regimes and available irrigation. Grasslands increased on the Wallender parcel by 21% (65 acres), 23% (71 acres) at the Simonis site, and 39% (62 acres) at Conley Lake. The emergent wetland cover type also changed significantly increasing 60% (184 acres) at Wallender and 59% (184 acres) on the Simonis tract. Today, agriculture lands (crop and grazed pasture) have been nearly eliminated from Bonneville Power Administration (BPA) mitigation project lands located on the LMWA.

  7. Habitat Evaluation Procedures (HEP) Report : Oxbow Conservation Area, 2002-2005 Technical Report.

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    Cochran, Brian

    2005-02-01

    This Habitat Evaluation Procedure (HEP) study was performed to determine baseline habitat units on the Oxbow Conservation Area in Grant County, Oregon. The evaluation is a required part of the Memorandum of Agreement between the Confederated Tribes of the Warm Springs and Bonneville Power Administration (BPA) relating to the acquisition and management of the Oxbow Conservation Area. The HEP team was comprised of individuals from the Washington Department of Fish and Wildlife and the Confederated Tribes of the Warm Springs Reservation of Oregon. The survey was conducted using the following HEP evaluation models for key species: black-capped chickadee (Poecile atricapilla), mallard (Anas platyrhynchos), mink (Mustela vison), western meadowlark (Sturnella neglecta), white-tailed deer (Odocoileus virginiana), and yellow warbler (Dendroica petechia). Cover types used in this survey were conifer forest, irrigated meadow, riparian meadow, upland meadow, riparian shrub, upland shrub, and mine tailings. The project generated 701.3 habitat units for mitigation crediting purposes. Results for each HEP species are: (1) Black-capped chickadee habitat was good, with only isolated areas lacking snags or having low tree canopy cover. (2) Mallard habitat was poor in upland meadows and marginal elsewhere due to a lack of herbaceous/shrub cover and low herbaceous height. (3) Mink habitat was good, limited only by the lack of the shrub component. (4) Western meadowlark habitat was marginal in upland meadow and mine tailing cover types and good in irrigated meadow. Percent cover of grass and height of herbaceous variables were limiting factors. (5) White-tailed deer habitat was marginal due to relatively low tree canopy cover, reduced shrub cover, and limited browse diversity. (6) Yellow Warbler habitat was marginal due to less than optimum shrub height and the lack of hydrophytic shrubs. General ratings (poor, marginal, etc.) are described in the introduction section.

  8. Habitat Evaluation Procedures (HEP) Report; Burlington Bottoms, Technical Report 1993-2003.

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    Beilke, Susan

    1993-08-01

    Burlington Bottoms, consisting of approximately 417 acres of riparian and wetland habitat, was purchased by the Bonneville Power Administration in November 1991. The site is located approximately 1/2 mile north of the Sauvie Island Bridge (T2N R1W Sections 20, 21), and is bound on the east side by Multnomah Channel and on the west side by the Burlington Northern Railroad right-of-way and U.S. Highway 30 (Figures 1 and 2). Wildlife habitat values resulting from the purchase of this site will contribute toward the goal of mitigating for habitat lost as outlined in the Columbia and Willamette River Basin's Fish and Wildlife Program and Amendments. Under this Program, mitigation goals were developed as a result of the loss of wildlife habitat due to the development and operation of Federal hydro-electric facilities in the Columbia and Willamette River Basins. In 1993, an interdisciplinary team was formed to develop and implement quantitative Habitat Evaluation Procedures (HEP) to document the value of various habitats at Burlington Bottoms. Results of the HEP will be used to: (1) determine the current status and habitat enhancement potential of the site consistent with wildlife mitigation goals and objectives; and (2) develop a management plan for the area. HEP participants included; Charlie Craig, BPA; Pat Wright, Larry Rasmussen, and Ron Garst, U. S. Fish and Wildlife Service; John Christy, The Nature Conservancy; and Doug Cottam, Sue Beilke, and Brad Rawls, Oregon Department of Fish and Wildlife.

  9. Habitat Evaluation Procedures (HEP) Report; Forrest Conservation Area, Technical Report 2003-2004.

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    Smith, Brent

    2005-01-01

    The Habitat Evaluation Procedure (HEP) study was performed to determine baseline habitat units on the 4,232-acre Forrest Conservation Area managed by the Confederated Tribes of Warm Springs Reservation of Oregon (Tribe) in Grant County, Oregon. The habitat evaluation is required as part of the Memorandum of Agreement between the Confederated Tribes of the Warm Springs and Bonneville Power Administration. Representatives from the Washington Department of Fish and Wildlife and the Tribes conducted the field surveys for the HEP. The survey collected data for habitat variables contained in habitat suitability index (HIS) models for wildlife species; the key species were black-capped chickadee (Poecile atricapilla), mallard (Anas platyrhynchos), mink (Mustela vison), western meadowlark (Sturnella neglecta), mule deer (Odocoileus hemionus), California Quail (Callipepla californica), and yellow warbler (Dendroica petechia). Cover types surveyed were grassland, meadow grassland, conifer forest, riparian tree shrub, shrub steppe, juniper forest, and juniper steppe. Other cover types mapped, but not used in the models were open water, roads, gravel pits, corrals, and residential.

  10. Red River Wildlife Management Area HEP Report, Habitat Evaluation Procedures, Technical Report 2004.

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    Ashley, Paul

    2004-11-01

    A habitat evaluation procedures (HEP) analysis conducted on the 314-acre Red River Wildlife Management Area (RRWMA) managed by the Idaho Department of Fish and Game resulted in 401.38 habitat units (HUs). Habitat variables from six habitat suitability index (HSI) models, comprised of mink (Mustela vison), mallard (Anas platyrhynchos), common snipe (Capella gallinago), black-capped chickadee (Parus altricapillus), yellow warbler (Dendroica petechia), and white-tailed deer (Odocoileus virginianus), were measured by Regional HEP Team (RHT) members in August 2004. Cover types included wet meadow, riverine, riparian shrub, conifer forest, conifer forest wetland, and urban. HSI model outputs indicate that the shrub component is lacking in riparian shrub and conifer forest cover types and that snag density should be increased in conifer stands. The quality of wet meadow habitat, comprised primarily of introduced grass species and sedges, could be improved through development of ephemeral open water ponds and increasing the amount of persistent wetland herbaceous vegetation e.g. cattails (Typha spp.) and bulrushes (Scirpus spp.).

  11. Habitat Evaluation Procedures (HEP) Report; Iskuulpa Wildlife Mitigation and Watershed Project, Technical Report 1998-2003.

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    Quaempts, Eric

    2003-01-01

    U.S. Fish and Wildlife Service (USFWS) Habitat Evaluation Procedures (HEP) were used to determine the number of habitat units credited to evaluate lands acquired and leased in Eskuulpa Watershed, a Confederated Tribes of the Umatilla Indian Reservation watershed and wildlife mitigation project. The project is designed to partially credit habitat losses incurred by BPA for the construction of the John Day and McNary hydroelectric facilities on the Columbia River. Upland and riparian forest, upland and riparian shrub, and grasslands cover types were included in the evaluation. Indicator species included downy woodpecker (Picuides puhescens), black-capped chickadee (Pams atricopillus), blue grouse (Beadragapus obscurus), great blue heron (Ardea herodias), yellow warbler (Dendroica petschia), mink (Mustela vison), and Western meadowlark (Sturnello neglects). Habitat surveys were conducted in 1998 and 1999 in accordance with published HEP protocols and included 55,500 feet of transects, 678 m2 plots, and 243 one-tenth-acre plots. Between 123.9 and f 0,794.4 acres were evaluated for each indicator species. Derived habitat suitability indices were multiplied by corresponding cover-type acreages to determine the number of habitat units for each species. The total habitat units credited to BPA for the Iskuulpa Watershed Project and its seven indicator species is 4,567.8 habitat units. Factors limiting habitat suitability are related to the direct, indirect, and cumulative effects of past livestock grazing, road construction, and timber harvest, which have simplified the structure, composition, and diversity of native plant communities. Alternatives for protecting and improving habitat suitability include exclusion of livestock grazing or implementation of restoration grazing schemes, road de-commissioning, reforestation, large woody debris additions to floodplains, control of competing and unwanted vegetation, reestablishing displaced or reduced native vegetation species

  12. Habitat Evaluation Procedures (HEP) Report : Hellsgate Project, 1999-2000 Technical Report.

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    Berger, Matthew

    2000-05-01

    A Habitat Evaluation Procedure (HEP) study was conducted on lands acquired and/or managed (4,568 acres total) by the Hellsgate Big Game Winter Range Wildlife Mitigation Project (Hellsgate project) to mitigate some of the losses associated with the original construction and operation of Grand Coulee Dam and inundation of habitats behind the dams. Three separate properties, totaling 2,224 acres were purchased in 1998. One property composed of two separate parcels, mostly grassland lies southeast of the town of Nespelem in Okanogan County (770 acres) and was formerly called the Hinman property. The former Hinman property lies within an area the Tribes have set aside for the protection and preservation of the sharp-tailed grouse (Agency Butte unit). This special management area minus the Hinman acquisition contains 2,388 acres in a long-term lease with the Tribes. The second property lies just south of the Silver Creek turnoff (Ferry County) and is bisected by the Hellsgate Road (part of the Friedlander unit). This parcel contains 60 acres of riparian and conifer forest cover. The third property (now named the Sand Hills unit) acquired for mitigation (1,394 acres) lies within the Hellsgate Reserve in Ferry County. This new acquisition links two existing mitigation parcels (the old Sand Hills parcels and the Lundstrum Flat parcel, all former Kuehne purchases) together forming one large unit. HEP team members included individuals from the Colville Confederated Tribes Fish and Wildlife Department (CTCR), Washington Department of Fish and Wildlife (WDFW), and Bureau of Land Management (BLM). The HEP team conducted a baseline habitat survey using the following HEP species models: mule deer (Odocoileus hemionus), mink (Mustela vison), downy woodpecker (Picoides pubescens), bobcat (Lynx rufus), yellow warbler (Dendroica petechia), and sharp-tailed grouse (Tympanuchus phasianellus columbianus). HEP analysis and results are discussed within the body of the text. The cover types

  13. Habitat Evaluation Procedures (HEP) Report; Precious Lands Wildlife Management Area, Technical Report 2000-2003.

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    Kozusko, Shana

    2003-12-01

    The Nez Perce Tribe (NPT) currently manages a 15,325 acre parcel of land known as the Precious Lands Wildlife Management Area that was purchased as mitigation for losses incurred by construction of the four lower Snake River dams. The Management Area is located in northern Wallowa County, Oregon and southern Asotin County, Washington (Figure 1). It is divided into three management parcels--the Buford parcel is located on Buford Creek and straddles the WA-OR state line, and the Tamarack and Basin parcels are contiguous to each other and located between the Joseph Creek and Cottonwood Creek drainages in Wallowa County, OR. The project was developed under the Pacific Northwest Electric Power Planning and Conservation Act of 1980 (P.L. 96-501), with funding from the Bonneville Power Administration (BPA). The acreage protected under this contract will be credited to BPA as habitat permanently dedicated to wildlife and wildlife mitigation. A modeling strategy known as Habitat Evaluation Procedure (HEP) was developed by the U.S. Fish and Wildlife Service and adopted by BPA as a habitat equivalency accounting system. Nine wildlife species models were used to evaluate distinct cover type features and provide a measure of habitat quality. Models measure a wide range of life requisite variables for each species and monitor overall trends in vegetation community health and diversity. One product of HEP is an evaluation of habitat quality expressed in Habitat Units (HUs). This HU accounting system is used to determine the amount of credit BPA receives for mitigation lands. After construction of the four lower Snake River dams, a HEP loss assessment was conducted to determine how many Habitat Units were inundated behind the dams. Twelve target species were used in that evaluation: Canada goose, mallard, river otter, downy woodpecker, song sparrow, yellow warbler, marsh wren, western meadowlark, chukar, ring-necked pheasant, California quail, and mule deer. The U.S. Army Corp of

  14. Habitat Evaluation Procedures (HEP) Report : Rainwater Wildlife Area, 1998-2001 Technical Report.

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    Childs, Allen

    2004-01-01

    The 8,768 acre Rainwater Wildlife Area was acquired in September 1998 by the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) through an agreement with Bonneville Power Administration (BPA) to partially offset habitat losses associated with construction of the John Day and McNary hydroelectric facilities on the mainstem Columbia River. U.S. Fish and Wildlife Service (USFWS) Habitat Evaluation Procedures (HEP) were used to determine the number of habitat units credited to BPA for acquired lands. Upland and riparian forest, upland and riparian shrub, and grassland rover types are evaluated in this study. Targeted wildlife species include downy woodpecker (Picoides pubescens), black-capped chickadee (Parus atricopillus), blue grouse (Dendragapus obscurus), great blue heron (Ardea herodias), yellow warbler (Dendroica petechia), mink (Mustela vison), and Western meadowlark (Sturnella neglects). Habitat surveys were conducted in 1998 and 1999 in accordance with published HEP protocols and included 65,300, 594m{sup 2} plots, and 112 one-tenth-acre plots. Between 153.3 and 7,187.46 acres were evaluated for each target wildlife mitigation species. Derived habitat suitability indices were multiplied by corresponding cover-type acreages to determine the number of habitat units for each species. The total baseline habitat units credited to BPA for the Rainwater Wildlife Area and its seven target species is 5,185.3 habitat units. Factors limiting habitat suitability are related to the direct, indirect, and cumulative effects of past livestock grazing, road construction, and timber harvest which have simplified the structure, composition, and diversity of native plant communities. Alternatives for protecting and improving habitat suitability include exclusion of livestock grazing, road de-commissioning/obliteration, reforestation and thinning, control of competing and unwanted vegetation (including noxious weeds), reestablishing displaced or reduced native

  15. Habitat Evaluation Procedures (HEP) Report; Steigerwald Lake National Wildlife Refuge, Technical Report 2000-2001.

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    Allard, Donna

    2001-09-01

    Steigenvald Lake National Wildlife Refuge (NWR, refuge) was established as a result of the U. S. Army Corps of Engineers (COE) transferring ownership of the Stevenson tract located in the historic Steigerwald Lake site to the U.S. Fish and Wildlife Service (FWS, Service) for the mitigation of the fish and wildlife losses associated with the construction of a second powerhouse at the Bonneville Dam on the Columbia River and relocation of the town of North Bonneville (Public Law 98-396). The construction project was completed in 1983 and resulted in the loss of approximately 577 acres of habitat on the Washington shore of the Columbia River (USFWS, 1982). The COE determined that acquisition and development of the Steigenvald Lake area, along with other on-site project management actions, would meet their legal obligation to mitigate for these impacts (USCOE, 1985). Mitigation requirements included restoration and enhancement of this property to increase overall habitat diversity and productivity. From 1994 to 1999, 317 acres of additional lands, consisting of four tracts of contiguous land, were added to the original refuge with Bonneville Power Administration (BPA) funds provided through the Washington Wildlife Mitigation Agreement. These tracts comprised Straub (191 acres), James (90 acres), Burlington Northern (27 acres), and Bliss (9 acres). Refer to Figure 1. Under this Agreement, BPA budgeted $2,730,000 to the Service for 'the protection, mitigation, and enhancement of wildlife and wildlife habitat that was adversely affected by the construction of Federal hydroelectric dams on the Columbia River or its tributaries' in the state of Washington (BPA, 1993). Lands acquired for mitigation resulting from BPA actions are evaluated using the habitat evaluation procedures (HEP) methodology, which quantifies how many Habitat Units (HUs) are to be credited to BPA. HUs or credits gained lessen BPA's debt, which was formally tabulated in the Federal Columbia

  16. Habitat Evaluation Procedures (HEP) Report; Willow Creek, Technical Report 1993-1994.

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    Beilke, Susan

    1994-09-01

    The Willow Creek site is one of the most significant remaining areas of typical native Willamette Valley habitats, with a variety of wetlands, grasslands, and woodlands. A diverse array of native flora and fauna, with significant wildlife habitats, is present on the site. Wildlife diversity is high, and includes species of mammals, songbirds, raptors, reptiles, amphibians, and one rare invertebrate. Over 200 species of native plants have been identified (including populations of six rare, threatened, or endangered species), along with significant remnants of native plant communities. Willow Creek is located in Lane County, Oregon, on the western edge of the City of Eugene (see Figure 1). The city limit of Eugene passes through the site, and the site is entirely within the Eugene Urban Growth Boundary (UGB). At present, only lands to the east and northeast of the site are developed to full urban densities. Low density rural residential and agricultural land uses predominate on lands to the northwest and south. A partially completed light industrial/research office park is located to the northwest. Several informal trails lead south from West 18th at various points into the site. The area encompasses a total of approximately 349 acres under several ownerships, in sections 3 and 4 of Township 18 South, Range 4 West. wildlife habitat values resulting from the purchase of this site will contribute toward the goal of mitigating for habitat lost as outlined in the Bonneville Power Administration's (BPA) Mitigation and Enhancement Plan for the Willamette River Basin. Under this Plan, mitigation goals were developed as a result of the loss of wildlife habitat due to the development and operation of Federal hydro-electric facilities in the Willamette River Basin. Results of the HEP will be used to: (1) determine the current status and habitat enhancement potential of the site consistent with wildlife mitigation goals and objectives; and (2) develop a management plan for

  17. Ecological Impact Assessment of Isfahan’s West Ringway on Ghamishloo Wildlife Refuge Using Habitat Evaluation Procedure (HEP

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    T. Makki

    2013-03-01

    Full Text Available Development of roads through protected areas and ecological sensitive regions can have catastrophic effects on wildlife. In Iran, road construction in sensitive habitats and protected areas has been expanding during the past decades. This study focuses on the ecological impacts of Isfahan’s west ringway, which passes through Ghamishloo wildlife refuge, I.U.C.N category IV, in Isfahan Province. The key affected species of the study area goitered gazelle (Gazella subgutturosa subgutterosa, was considered for impact analysis. We used HEP (Habitat Evaluation Procedure as a habitat-based impact assessment methodology which considers habitat quality and quantity. Habitat quality was measured as habitat suitability index for the species. By literature review and field observations (293 presence points, five effective variables in habitat suitability including vegetation cover, slope, elevation, distance to water and distance to road were identified, and habitat units (HUs were derived from multiplying the HSI for goitered gazelle by the species habitat area at two times (before and after road construction. The results showed that due to the presence of the ringway, 7710 HUs for goitered gazelle have been lost. In addition, we used landscape ecology approach for quantifying landscape pattern change due to road construction and landscape metrics including NP (Number of Patches, MNN (Mean Nearest Neighbor and CONTAG (Contagion. Our results provided quantitative data on habitat loss and landscape fragmentation in Ghamishloo wildlife refuge and indicated negative impacts of the ringway on goitered gazelle populations by restricting their movement between habitat patches in this region, which presents a concern for the conservation of this vulnerable species.

  18. Habitat Evaluation Procedures (HEP) Report; Big Island - The McKenzie River, Technical Report 1998-2001.

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    Sieglitz, Greg

    2001-03-01

    The Big Island site is located in the McKenzie River flood plain, containing remnant habitats of what was once more common in this area. A diverse array of flora and fauna, representing significant wildlife habitats, is present on the site. Stands of undisturbed forested wetlands, along with riparian shrub habitats and numerous streams and ponds, support a diversity of wildlife species, including neotropical migratory songbirds, raptors, mammals, reptiles, and amphibians (including two State-listed Sensitive Critical species). The project is located in eastern Springfield, Oregon (Figure 1). The project area encompasses 187 acres under several ownerships in Section 27 of Township 17S, Range 2W. Despite some invasion of non-native species, the site contains large areas of relatively undisturbed wildlife habitat. Over several site visits, a variety of wildlife and signs of wildlife were observed, including an active great blue heron rookery, red-Legged frog egg masses, signs of beaver, and a bald eagle, Wildlife habitat values resulting from the purchase of this site will contribute toward the goal of mitigating for habitat lost as outlined in the Bonneville Power Administration's (BPA) Mitigation and Enhancement Plan for the Willamette River Basin. Under this Plan, mitigation goals and objectives were developed as a result of the loss of wildlife habitat due to the construction of Federal hydroelectric facilities in the Willamette River Basin. Results of the Habitat Evaluation Procedures (HEP) will be used to: (1) determine the current habitat status of the study area and habitat enhancement potential of the site consistent with wildlife mitigation goals and objectives; and (2) develop a management plan for the area.

  19. Habitat Evaluation Procedures (HEP) Report : Oleson Tracts of the Tualatin River National Wildlife Refuge, 2001-2002 Technical Report.

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    Allard, Donna; Smith, maureen; Schmidt, Peter

    2004-09-01

    Located in the northern Willamette River basin, Tualatin River National Wildlife Refuge (Refuge) was established in 1992 with an approved acquisition boundary to accommodate willing sellers with potentially restorable holdings within the Tualatin River floodplain. The Refuge's floodplain of seasonal and emergent wetlands, Oregon ash riparian hardwood, riparian shrub, coniferous forest, and Garry oak communities are representative of remnant plant communities historically common in the Willamette River valley and offer an opportunity to compensate for wildlife habitat losses associated with the Willamette River basin federal hydroelectric projects. The purchase of the Oleson Units as additions to the Refuge using Bonneville Power Administration (BPA) funds will partially mitigate for wildlife habitat and target species losses incurred as a result of construction and inundation activities at Dexter and Detroit Dams. Lands acquired for mitigation of Federal Columbia River Power System (FCRPS) impacts to wildlife are evaluated using the Habitat Evaluation Procedures (HEP) methodology, which quantifies how many Habitat Units (HUs) are to be credited to BPA. HUs or credits gained lessen BPA's debt, which was formally tabulated in the FCRPS Loss Assessments and adopted as part of the Northwest Power and Conservation Council's Fish and Wildlife Program as a BPA obligation (NWPCC, 1994 and 2000). There are two basic management scenarios to consider for this evaluation: (1) Habitats can be managed without restoration activities to benefit wildlife populations, or (2) Habitats can be restored using a number of techniques to improve habitat values more quickly. Without restoration, upland and wetland areas may be periodically mowed and disced to prevent invasion of exotic vegetation, volunteer trees and shrubs may grow to expand forested areas, and cooperative farming may be employed to provide forage for migrating and wintering waterfowl. Abandoned cropland

  20. Middle Rio Grande Bosque Ecosystem Restoration Feasibility Study Habitat Assessment Using Habitat Evaluation Procedures (HEP): Analyses, Results and Documentation

    Science.gov (United States)

    2012-08-01

    Findlay et al. 2002, Merkey 2003), and coral reefs (Jameson 1998). Reference-based approaches have also been used to evaluate ecosystems in a...Erdmann, G. R. Gibson, Jr., and Potts, K. W. 1998. Development of biological criteria for coral reef ecosystem assessment. U. S. Environmental...Yukon streams. Freshwater Biology 39:765-774. Barbour, M. T., J. Gerritsen, B. D. Snyder, and J. B. Stribling. 1999. Multimetric approach for

  1. Clear Creek Watershed Flood Risk Management Habitat Assessments Using Habitat Evaluation Procedures (HEP): Analyses, Results and Documentation

    Science.gov (United States)

    2013-07-01

    Brazori - Galveston .---. H . l__l = ’ > .,..,. - ~ :ana e.J I I I • ~ Dm.4l>)" Ktlly A. Bu1b-Copti to !:JillC1 Elm<-..ul l.obo< tto <F bnht t:SACI Oah...problems and take advantage of the opportunities, to evaluate potential effects of alternatives and to support decision-making...target years in both the environmental and economic analyses, and between the baseline and future analyses. In studies focused on long -term effects

  2. Habitat Evaluation Procedures Report; Carl Property - Yakama Nation.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul; Muse, Anthony

    2008-02-01

    A baseline habitat evaluation procedures (HEP) analysis was conducted on the Carl property (160 acres) in June 2007 to determine the number of habitat units to credit Bonneville Power Administration (BPA) for providing funds to acquire the property as partial mitigation for habitat losses associated with construction of McNary Dam. HEP surveys also helped assess the general ecological condition of the property. The Carl property appeared damaged from livestock grazing and exhibited a high percentage of invasive forbs. Exotic grasses, while present, did not comprise a large percentage of the available cover in most areas. Cover types were primarily grassland/shrubsteppe with a limited emergent vegetation component. Baseline HEP surveys generated 356.11 HUs or 2.2 HUs per acre. Habitat units were associated with the following HEP models: California quail (47.69 HUs), western meadowlark (114.78 HUs), mallard (131.93 HUs), Canada goose (60.34 HUs), and mink (1.38 HUs).

  3. Eder Acquisition 2007 Habitat Evaluation Procedures Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul R.

    2008-01-01

    A habitat evaluation procedures (HEP) analysis was conducted on the Eder acquisition in July 2007 to determine how many protection habitat units to credit Bonneville Power Administration (BPA) for providing funds to acquire the project site as partial mitigation for habitat losses associated with construction of Grand Coulee and Chief Joseph Dams. Baseline HEP surveys generated 3,857.64 habitat units or 1.16 HUs per acre. HEP surveys also served to document general habitat conditions. Survey results indicated that the herbaceous plant community lacked forbs species, which may be due to both livestock grazing and the late timing of the surveys. Moreover, the herbaceous plant community lacked structure based on lower than expected visual obstruction readings (VOR); likely a direct result of livestock impacts. In addition, introduced herbaceous vegetation including cultivated pasture grasses, e.g. crested wheatgrass and/or invader species such as cheatgrass and mustard, were present on most areas surveyed. The shrub element within the shrubsteppe cover type was generally a mosaic of moderate to dense shrubby areas interspersed with open grassland communities while the 'steppe' component was almost entirely devoid of shrubs. Riparian shrub and forest areas were somewhat stressed by livestock. Moreover, shrub and tree communities along the lower reaches of Nine Mile Creek suffered from lack of water due to the previous landowners 'piping' water out of the stream channel.

  4. Habitat Evaluation Procedures Report; Graves Property - Yakama Nation.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul; Muse, Anthony

    2008-02-01

    A habitat evaluation procedures (HEP) analysis was conducted on the Graves property (140 acres) in June 2007 to determine the number of habitat units to credit Bonneville Power Administration (BPA) for providing funds to acquire the property as partial mitigation for habitat losses associated with construction of McNary Dam. HEP surveys also documented the general ecological condition of the property. The Graves property was significantly damaged from past/present livestock grazing practices. Baseline HEP surveys generated 284.28 habitat units (HUs) or 2.03 HUs per acre. Of these, 275.50 HUs were associated with the shrubsteppe/grassland cover type while 8.78 HUs were tied to the riparian shrub cover type.

  5. Evaluation of the KEMRI Hep-cell II test kit for detection of hepatitis B ...

    African Journals Online (AJOL)

    Hepatitis B surface antigen (HBsAg) is one of the most important serological markers used to diagnose acute and chronic hepatitis B infection. The objective of the current evaluation was to assess the operational characteristics of the Kenya Medical Research Institute (KEMRI) Hep-cell II against an ELISA Exsym HBsAg in ...

  6. Design and evaluation of a hybrid storage system in HEP environment

    Science.gov (United States)

    Xu, Qi; Cheng, Yaodong; Chen, Gang

    2017-10-01

    Nowadays, the High Energy Physics experiments produce a large amount of data. These data are stored in mass storage systems which need to balance the cost, performance and manageability. In this paper, a hybrid storage system including SSDs (Solid-state Drive) and HDDs (Hard Disk Drive) is designed to accelerate data analysis and maintain a low cost. The performance of accessing files is a decisive factor for the HEP computing system. A new deployment model of Hybrid Storage System in High Energy Physics is proposed which is proved to have higher I/O performance. The detailed evaluation methods and the evaluations about SSD/HDD ratio, and the size of the logic block are also given. In all evaluations, sequential-read, sequential-write, random-read and random-write are all tested to get the comprehensive results. The results show the Hybrid Storage System has good performance in some fields such as accessing big files in HEP.

  7. HEP Applications Evaluation of the EDG Testbed and Middleware

    CERN Document Server

    Augustin, I; Closier, J; van Herwijnen, E; Blaising, J J; Boutigny, D; Charlot, C; Garonne, V; Tsaregorodtsev, A; Bos, K; Templon, J; Capiluppi, P; Fanfani, A; Barbera, R; Negri, G; Perini, L; Resconi, S; Sitta, M; Reale, M; Vicinanza, D; Bagnasco, S; Cerello, P; Sciaba, A; Smirnova, O; Colling, D; Harris, F; Burke, Stephen

    2003-01-01

    Workpackage 8 of the European Datagrid project was formed in January 2001 with representatives from the four LHC experiments, and with experiment independent people from five of the six main EDG partners. In September 2002 WP8 was strengthened by the addition of effort from BaBar and D0. The original mandate of WP8 was, following the definition of short- and long-term requirements, to port experiment software to the EDG middleware and testbed environment. A major additional activity has been testing the basic functionality and performance of this environment. This paper reviews experiences and evaluations in the areas of job submission, data management, mass storage handling, information systems and monitoring. It also comments on the problems of remote debugging, the portability of code, and scaling problems with increasing numbers of jobs, sites and nodes. Reference is made to the pioneeering work of Atlas and CMS in integrating the use of the EDG Testbed into their data challenges. A forward look is made t...

  8. Guidelines for hazard evaluation procedures

    National Research Council Canada - National Science Library

    2008-01-01

    ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxi 1 . Hazard Evaluation Procedures ... Management Overview ... ... Part I Preface 11 Introduction to the Guidelines 1.1 Background ... 1.2 Relationship...

  9. Evaluating the scalability of HEP software and multi-core hardware

    CERN Document Server

    Jarp, S; Leduc, J; Nowak, A

    2011-01-01

    As researchers have reached the practical limits of processor performance improvements by frequency scaling, it is clear that the future of computing lies in the effective utilization of parallel and multi-core architectures. Since this significant change in computing is well underway, it is vital for HEP programmers to understand the scalability of their software on modern hardware and the opportunities for potential improvements. This work aims to quantify the benefit of new mainstream architectures to the HEP community through practical benchmarking on recent hardware solutions, including the usage of parallelized HEP applications.

  10. Dormaier and Chester Butte 2007 Follow-up Habitat Evaluation Procedures Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul R.

    2008-01-01

    Follow-up habitat evaluation procedures (HEP) analyses were conducted on the Dormaier and Chester Butte wildlife mitigation sites in April 2007 to determine the number of additional habitat units to credit Bonneville Power Administration (BPA) for providing funds to enhance, and maintain the project sites as partial mitigation for habitat losses associated with construction of Grand Coulee Dam. The Dormaier follow-up HEP survey generated 482.92 habitat units (HU) or 1.51 HUs per acre for an increase of 34.92 HUs over baseline credits. Likewise, 2,949.06 HUs (1.45 HUs/acre) were generated from the Chester Butte follow-up HEP analysis for an increase of 1,511.29 habitat units above baseline survey results. Combined, BPA will be credited with an additional 1,546.21 follow-up habitat units from the Dormaier and Chester Butte parcels.

  11. Evaluation of anti-hepatocarcinoma capacity of puerarin nanosuspensions against human HepG2 cells

    Science.gov (United States)

    Meng, Xiang-Ping; Zhang, Zhen; Wang, Yi-Fei; Wang, Zhi-ping; Chen, Tong-sheng

    2017-02-01

    Hepatocarcinoma, a malignant cancer, threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma to chemotherapy. Puerarin (Pue), a major active ingredient in the traditional Chinese medicine Gegen, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Pue nanosuspension (Pue-NS) composed of Pue and poloxamer 188 was prepared by high pressure homogenization technique. The in vitro anti-hepatocarcinoma effects of Pue-NS relative to efficacy of bulk Pue were evaluated. The particle size and zeta potential of Pue-NS were 218.5 nm and -18.8 mV, respectively. MTT assay showed that Pue-NS effectively inhibited the proliferation of HepG2 cells, and the corresponding IC50 values of Pue-NS and bulk Pue were 3.39 and 5.73 μg/ml. These results suggest that the delivery of Pue-NS is a promising approach for treating tumors.

  12. Arthroscopic latarjet procedure: safety evaluation in cadavers

    National Research Council Canada - National Science Library

    Gracitelli, Mauro Emilio Conforto; Ferreira, Arnaldo Amado; Benegas, Eduardo; Malavolta, Eduardo Angeli; Sunada, Edwin Eiji; Assunção, Jorge Henrique

    2013-01-01

    To evaluate the safety of arthroscopic Latarjet procedure in cadavers. : Twelve cadaveric shoulders underwent arthroscopic Latarjet procedure in our laboratory for arthroscopy, by four different surgeons...

  13. West Foster Creek Expansion Project 2007 HEP Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul R.

    2008-02-01

    During April and May 2007, the Columbia Basin Fish and Wildlife Authority's (CBFWA) Regional HEP Team (RHT) conducted baseline Habitat Evaluation Procedures (HEP) (USFWS 1980, 1980a) analyses on five parcels collectively designated the West Foster Creek Expansion Project (3,756.48 acres). The purpose of the HEP analyses was to document extant habitat conditions and to determine how many baseline/protection habitat units (HUs) to credit Bonneville Power Administration (BPA) for funding maintenance and enhancement activities on project lands as partial mitigation for habitat losses associated with construction of Grand Coulee and Chief Joseph Dams. HEP evaluation models included mule deer (Odocoileus hemionus), western meadowlark (Sturnella neglecta), sharp-tailed grouse, (Tympanuchus phasianellus), Bobcat (Lynx rufus), mink (Neovison vison), mallard (Anas platyrhynchos), and black-capped chickadee (Parus atricapillus). Combined 2007 baseline HEP results show that 4,946.44 habitat units were generated on 3,756.48 acres (1.32 HUs per acre). HEP results/habitat conditions were generally similar for like cover types at all sites. Unlike crediting of habitat units (HUs) on other WDFW owned lands, Bonneville Power Administration received full credit for HUs generated on these sites.

  14. Hybrid Microcircuit Rework Procedures Evaluation.

    Science.gov (United States)

    1980-08-01

    solder bonding of active device chips has been reported by Himmel .(8) The overall objective of this study was to determine which die bonding process steps...Tech., Sept. 1975, pp. 45-48. ()Hybrid Microcircuit In-Process Qualification, Final Report, Aug. 1976, Contract No. ECOM-75-1311-F, R. P. Himmel ...substrate bonding pad metallization. Table 4 lists the materials variables employed. Table 5 lists all the material combinations evaluated by Himmel

  15. Habitat Evaluation Procedures (HEP) Report : Vancouver Lowlands Shillapoo Wildlife Area, 1994-1995 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Calkins, Brian; Anderson, Eric; Ashley, Paul

    1995-01-01

    This project was conducted as part of a comprehensive planning effort for the Vancouver Lowlands project area. The study was funded by The Bonneville Power Administration (BPA) and carried out by the Washington Department of Fish and Wildlife (WDFW). The Vancouver Lowlands is considered an area of high priority by WDFW and is being considered as a potential site for wildlife mitigation activities by BPA. The objectives of this study were to collect baseline information and determine current habitat values for the study area. A brief discussion of potential future management and a proposed listing of priorities for habitat protection are found near the end of this report. This report is a companion to a programmatic management plan being drafted for the area which will outline specific, management programs to improve habitat conditions based, in part, on this study. The following narratives, describing limiting habitat variables, carry recurring themes for each indicator species and habitat type. These recurring variables that limited habitat value include: Waterbodies that lack emergent and submerged vegetation; forest areas that lack natural shrub layers; a predominance of non-hydrophytic and less desirable non-native plants where shrubs are present; a general lack of cover for ground nesting and secure waterfowl nest sites (island type). Human disturbance was the variable that varied more than any other from site to site in the study area. One issue that the models we used do not truly deal with is the quantity and connectivity of habitat. The mallard and heron models deal with spatial relationships but for other species this may be as critical. Observation of habitat maps easily show that forested habitats are in short supply. Their continuity along Lake river and the Columbia has been broken by past development. Wetland distribution has also been affected by past development.

  16. Habitat Evaluation Procedures (HEP) Report; Tacoma/Trimble Area Management Plan, Technical Report 2001-2003.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray; Lockwood, Jr., Neil; Holmes, Darren

    2003-10-01

    In 2000 and 2001, the Kalispel Natural Resource Department (KNRD) continued to mitigate the wildlife habitat losses as part of the Albeni Falls Wildlife Mitigation Project. Utilizing Bonneville Power Administration (BPA) funds, the Kalispel Tribe of Indians (Tribe) purchased three projects totaling nearly 1,200 acres. The Tacoma/Trimble Wildlife Management Area is a conglomeration of properties now estimated at 1,700 acres. It is the Tribe's intent to manage these properties in cooperation and collaboration with the Pend Oreille County Public Utility District (PUD) No. 1 and the U.S. Fish and Wildlife Service (USFWS) to benefit wildlife habitats and associated species, populations, and guilds.

  17. Recommended evaluation procedure for photonuclear cross section

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Young-Ouk; Chang, Jonghwa; Fukahori, Tokio [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1998-03-01

    In order to generate photonuclear cross section library for the necessary applications, data evaluation is combined with theoretical evaluation, since photonuclear cross sections measured cannot provide all necessary data. This report recommends a procedure consisting of four steps: (1) analysis of experimental data, (2) data evaluation, (3) theoretical evaluation and, if necessary, (4) modification of results. In the stage of analysis, data obtained by different measurements are reprocessed through the analysis of their discrepancies to a representative data set. In the data evaluation, photonuclear absorption cross sections are evaluated via giant dipole resonance and quasi-deutron mechanism. With photoabsorption cross sections from the data evaluation, theoretical evaluation is applied to determine various decay channel cross sections and emission spectra using equilibrium and preequilibrium mechanism. After this, the calculated results are compared with measured data, and in some cases the results are modified to better describe measurements. (author)

  18. 34 CFR 300.304 - Evaluation procedures.

    Science.gov (United States)

    2010-07-01

    ... the child to be involved in and progress in the general education curriculum (or for a preschool child... 34 Education 2 2010-07-01 2010-07-01 false Evaluation procedures. 300.304 Section 300.304 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION...

  19. Training Procedure to Evaluate Visible Emissions.

    Science.gov (United States)

    West Virginia Air Pollution Control Commission, Charleston.

    Described in this pamphlet is a procedure used by the West Virginia Air Pollution Control Commission to train personnel in evaluating visible emissions. For this purpose a "Smoke Observer's Training Unit" has been designed, a machine capable of generating both gray/black plumes for training in the use of Ringlemann readings and white plumes for…

  20. Shillapoo Wildlife Area 2007 Follow-up HEP Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul R.

    2008-03-01

    In April and May 2007 the Regional HEP Team (RHT) conducted a follow-up HEP analysis on the Egger (612 acres) and Herzog (210 acres) parcels located at the north end of the Shillapoo Wildlife Area. The Egger and Herzog parcels have been managed with Bonneville Power Administration funds since acquired in 1998 and 2001 respectively. Slightly more than 936 habitat units (936.47) or 1.14 HUs per acre was generated as an outcome of the 2007 follow-up HEP surveys. Results included 1.65 black-capped chickadee HUs, 280.57 great blue heron HUs, 581.45 Canada goose HUs, 40 mallard HUs, and 32.80 mink HUs. Introduction A follow-up Habitat Evaluation Procedures (HEP) (USFWS 1980) analysis was conducted by the Columbia Basin Fish and Wildlife Authority's (CBFWA) Regional HEP Team (RHT) during April and May 2007 to document changes in habitat quality and to determine the number of habitat units (HUs) to credit Bonneville Power Administration (BPA) for providing operation and maintenance (O&M) funds since WDFW acquired the parcels. The 2007 follow-up HEP evaluation was limited to Shillapoo Wildlife Area (SWA) parcels purchased with Bonneville Power Administration funds. D. Budd (pers. comm.) reported WDFW purchased the 612 acre Egger Farms parcel on November 2, 1998 for $1,737,0001 and the 210 acre Herzog acquisition on June 21, 2001 for $500,000 with Memorandum of Agreement funds (BPA and WDFW 1996) as partial fulfillment of BPA's wildlife mitigation obligation for construction of Bonneville and John Day Dams (Rasmussen and Wright 1989). Anticipating the eventual acquisition of the Egger and Herzog properties, WDFW conducted HEP surveys on these lands in 1994 to determine the potential number of habitat units to be credited to BPA. As a result, HEP surveys and habitat unit calculations were completed as much as seven years prior to acquiring the sites. The term 'Shillapoo Wildlife Area' will be used to describe only the Herzog and Egger parcels in this

  1. [Evaluation of reactivity and immunogenicity of a cultured concentrated inactivated vaccine against hepatitis A "Hep-A-In-Vak"].

    Science.gov (United States)

    Gorbunov, M A; Pavlova, L I; Karpovich, L G; Kalashnikova, T V; Bektimirov, T A; Levina, V D; Maĭdaniuk, A G; Ermolaeva, T N; Shervarli, V I; Balashev, B P

    1995-01-01

    Clinical trials of inactivated hepatitis A vaccine Hep-A-in-Vac demonstrated its specific safety and moderate reactogenicity, manifesting by short-term fibrillar twitching of musculus deltoideus at the site of injection. After a course of three immunizations 87.5% seronegative vaccines developed a high level of specific antibodies to hepatitis A virus with at least 100 reverse values of titers. In controls antibody titers remained seronegative in 90% cases. These data indicate evident immunologic activity of Hep-A-in-Vac vaccine.

  2. 29 CFR 1956.22 - Procedures for evaluation and monitoring.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Procedures for evaluation and monitoring. 1956.22 Section..., Change, Evaluation and Withdrawal of Approval Procedures § 1956.22 Procedures for evaluation and monitoring. The procedures contained in part 1954 of this chapter shall be applicable to evaluation and...

  3. Antigens evaluation of Fasciola hepática for three inmulogic methods

    Directory of Open Access Journals (Sweden)

    Erasmo Colona

    2014-06-01

    Full Text Available Excretion–secretion antigens (ESA of Fasciola hepatica were evaluated in bovine feces samples from endemic area (Andrés Avelino Cáceres, Central Peruvian Andean. We employed ELISA (Fascidig, Indirect hemagglutination (IHA and Latex agglutination (LA; the results were correlationated with Dennis coproparasitologic method (DC. A number of 106 bovine feces samples were obtained from diferent places of the Region between October and November 1995 (rain time. AES was not detected for LA, the specificity for IHA was 63,6% and the sensibility 35% in relation to DC; the specificity for Elisa (Fascidig was 68,2% and the sensibility was 60% with regard to DC; and in relation to IHA the specificity was 61,7% and the sensibility 50%. The correlation between ELISA (Fascidig and DC was significative, it indicates that ELISA is the best method for the fasciolosis diagnostic in the prepatent and patent phases, while DC only is recommended for the patent phase diagnostic. Diagnostic of infection or prevalence for IHA, ELISA and DC was 35,8%, 42,5% and 37,7% respectively. The results indicate that in the investigated area the prevalence of Fasciola hepatica is high. Thanks to this investigation it was possible to evaluate three immunologic methods and to determine the performance of ELISA-Fascidig for the diagnostic of fasciolosis both prepatent and patent infection phases by means of the demonstration of coproantigens.

  4. Avaliação clínico-laboratorial de pacientes com síndrome antifosfolípide primária segundo a frequência de anticorpos antinucleares (FAN Hep-2 Clinical and laboratory evaluation of patients with primary antiphospholipid syndrome according to the frequency of antinuclear antibodies (ANA Hep-2

    Directory of Open Access Journals (Sweden)

    Jozélio Freire de Carvalho

    2010-06-01

    Full Text Available OBJETIVO: Avaliar a frequência de manifestações clínicas e laboratoriais em pacientes com síndrome antifosfolípide primária (SAFP com anticorpos antinucleares positivos (FAN Hep-2+, comparados àqueles com esses anticorpos negativos (FAN Hep-2 -. PACIENTES E MÉTODOS: Estudo transversal em 58 pacientes (82,8% mulheres com SAFP. Foram avaliados os dados demográficos, clínicos, comorbidades, medicações e anticorpos antifosfolípides. RESULTADOS: Dos 58 pacientes incluídos no estudo, vinte (34,5% apresentaram presença de FAN Hep-2. Comparando-se o grupo de pacientes FAN Hep-2+ com aqueles FAN Hep-2 -, verificou-se que ambos os grupos de pacientes com SAFP não diferiram estatisticamente em relação aos dados demográficos, bem como em relação ao tempo de doença. Em relação às manifestações clínicas e laboratoriais, o grupo com FAN Hep-2 + apresentou maior frequência de trombose venosa profunda (85 versus 52,6%, P = 0,04, uma frequência estatística e significativamente maior de anticardiolipina IgG (85 versus 52,6%, P = 0,02 e uma tendência para anticardiolipina IgM (80% versus 52,6%, P = 0,05, bem como maiores medianas desses anticorpos [33 (0-128 versus 20 (0-120 GPL, P = 0,008] e [33 (0-120 versus 18,5 (0-120 MPL, P = 0,009]. Tal diferença não foi observada no que se refere a outras manifestações da SAF, presença de comorbidades, estilo de vida e uso de medicações. CONCLUSÃO: Pacientes com SAFP que apresentam FAN Hep-2+ têm maior frequência de trombose venosa profunda e anticardiolipinas IgG e IgM.OBJECTIVE: To evaluate the frequency of clinical and laboratory manifestations in patients with primary antiphospholipid syndrome (PAPS with positive antinuclear antibodies (ANA Hep-2+ compared to those in whom this antibody is negative (ANA Hep-2-. PATIENTS AND METHODS: This is a transversal study with 58 patients (82.8% females with PAPS. Demographic and clinical data, comorbidities, medications, and

  5. Columbia River Wildlife Mitigation Habitat Evaluation Procedures Report / Scotch Creek Wildlife Area, Berg Brothers, and Douglas County Pygmy Rabbit Projects.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul R.

    1997-01-01

    This Habitat Evaluation Procedure study was conducted to determine baseline habitat units (HUs) on the Scotch Creek, Mineral Hill, Pogue Mountain, Chesaw and Tunk Valley Habitat Areas (collectively known as the Scotch Creek Wildlife Area) in Okanogan County, Sagebrush Flat and the Dormaler property in Douglas County, and the Berg Brothers ranch located in Okanogan County within the Colville Reservation. A HEP team comprised of individuals from the Washington Department of Fish and Wildlife, the Confederated Tribes of the Colville Reservation, and the Natural Resources Conservation Service (Appendix A) conducted baseline habitat surveys using the following HEP evaluation species: mule deer (Odocoileus hemionus), sharp-tailed grouse (Tympanuchus phasianellus), pygmy rabbit (Brachylagus idahoensis), white-tailed deer (Odocoileus virginiana), mink (Mustela vison), Canada goose (Branta canadensis), downy woodpecker (Picoides pubescens), Lewis woodpecker (Melanerpes lewis), and Yellow warbler (Dendroica petechia). Results of the HEP analysis are listed below. General ratings (poor, marginal, fair, etc.,) are described in Appendix B. Mule deer habitat was marginal lacking diversity and quantify of suitable browse species. Sharp-tailed grouse habitat was marginal lacking residual nesting cover and suitable winter habitat Pygmy rabbit habitat was in fair condition except for the Dormaier property which was rated marginal due to excessive shrub canopy closure at some sites. This report is an analysis of baseline habitat conditions on mitigation project lands and provides estimated habitat units for mitigation crediting purposes. In addition, information from this document could be used by wildlife habitat managers to develop management strategies for specific project sites.

  6. An automated procedure for evaluating song imitation.

    Directory of Open Access Journals (Sweden)

    Yael Mandelblat-Cerf

    Full Text Available Songbirds have emerged as an excellent model system to understand the neural basis of vocal and motor learning. Like humans, songbirds learn to imitate the vocalizations of their parents or other conspecific "tutors." Young songbirds learn by comparing their own vocalizations to the memory of their tutor song, slowly improving until over the course of several weeks they can achieve an excellent imitation of the tutor. Because of the slow progression of vocal learning, and the large amounts of singing generated, automated algorithms for quantifying vocal imitation have become increasingly important for studying the mechanisms underlying this process. However, methodologies for quantifying song imitation are complicated by the highly variable songs of either juvenile birds or those that learn poorly because of experimental manipulations. Here we present a method for the evaluation of song imitation that incorporates two innovations: First, an automated procedure for selecting pupil song segments, and, second, a new algorithm, implemented in Matlab, for computing both song acoustic and sequence similarity. We tested our procedure using zebra finch song and determined a set of acoustic features for which the algorithm optimally differentiates between similar and non-similar songs.

  7. Cytotoxic, genotoxic and biochemical markers of insecticide toxicity evaluated in human peripheral blood lymphocytes and an HepG2 cell line.

    Science.gov (United States)

    Želježić, Davor; Mladinić, Marin; Žunec, Suzana; Lucić Vrdoljak, Ana; Kašuba, Vilena; Tariba, Blanka; Živković, Tanja; Marjanović, Ana Marija; Pavičić, Ivan; Milić, Mirta; Rozgaj, Ružica; Kopjar, Nevenka

    2016-10-01

    This study evaluated the cyto- and genotoxic effects of three pesticides: α-cypermethrin, chlorpyrifos and imidacloprid applied in vitro to human lymphocytes and HepG2 cells for exposure times of 4 and 24 h at concentrations corresponding to OEL, ADI and REL. Assessments were made using oxidative stress biomarkers and the alkaline comet, cytokinesis-block micronucleus cytome and cell viability assays. Low doses of all three pesticides displayed DNA damaging potential, both in lymphocytes and HepG2 cells. At the tested concentrations, all three compounds induced lymphocyte apoptosis, though α-cypermethrin and chlorpyrifos were generally more cyto- and genotoxic than imidacloprid. At the tested concentrations, oxidative stress biomarkers were not significantly altered, and the effects mediated indirectly through free radicals may not have a key role in the formation of DNA damage. It is likely that the DNA damaging effects were caused by direct interactions between the tested compounds and/or their metabolites that destabilized the DNA structure. The tested pesticides had the potential for MN, NB and NPB formation and to disturb cell cycle kinetics in both cell types. There were also indications that exposure to α-cypermethrin led to the formation of crosslinks in DNA, though this would require more detailed study in the future. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. A procedure for evaluating environmental impact

    Science.gov (United States)

    Leopold, Luna Bergere; Clarke, Frank Eldridge; Hanshaw, Bruce B.; Balsley, James R.

    1971-01-01

    In a recent article in "Science" discussing the Environmental Policy Act of 1969, Gillette (1971) states "The law's instructions for preparing an impact report apparently are not specific enough to insure that an agency will fully, or even usefully, examine the environmental effects of the projects it plans." This report contains a procedure that may assist in developing uniform environmental impact statements. The Department of the Interior and the Council on Environmental Quality will appreciate comments on the procedure here proposed.

  9. A decision support system-based procedure for evaluation and ...

    Indian Academy of Sciences (India)

    This paper presents a novel integrated nature conservation management procedure developed to monitor and evaluate the sustainability of Mediterranean protected areas. This procedure was successfully implemented and formally evaluated by protected area managers in six Mediterranean countries, results of which are ...

  10. 34 CFR 303.166 - Evaluation, assessment, and nondiscriminatory procedures.

    Science.gov (United States)

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Evaluation, assessment, and nondiscriminatory procedures. 303.166 Section 303.166 Education Regulations of the Offices of the Department of Education... Statewide System-Application Requirements § 303.166 Evaluation, assessment, and nondiscriminatory procedures...

  11. Evaluation of medical radiation exposure in pediatric interventional radiology procedures

    Energy Technology Data Exchange (ETDEWEB)

    Navarro, Valeria Coelho Costa; Navarro, Marcus Vinicius Teixeira; Oliveira, Aline da Silva Pacheco, E-mail: vccnavarro@gmail.com [Instituto Federal de Educacao, Ciencia e Tecnologia da Bahia (IFBA), Salvador, BA (Brazil); Maia, Ana Figueiredo [Universidade Federal de Sergipe (UFS), Aracaju, SE (Brazil); Oliveira, Adriano Dias Dourado [Sociedade Brasileira de Hemodinamica e Cardiologia Intervencionista, Salvador, BA (Brazil)

    2012-07-15

    Objective: To evaluate pediatric radiation exposure in procedures of interventional radiology in two hospitals in the Bahia state, aiming at contributing to delineate the scenario at the state and national levels. The knowledge of exposure levels will allow an evaluation of the necessity of doses optimization, considering that peculiarities of radiology and pediatrics become even more significant in interventional radiology procedures which involve exposure to higher radiation doses. Materials and Methods: A total of 32 procedures were evaluated in four rooms of the two main hospitals performing pediatric interventional radiology procedures in the Bahia state. Air kerma rate and kerma-area product were evaluated in 27 interventional cardiac and 5 interventional brain procedures. Results: Maximum values for air kerma rate and kerma-area product and air kerma obtained in cardiac procedures were, respectively, 129.9 Gy.cm{sup 2} and 947.0 mGy; and, for brain procedures were 83.3 Gy.cm{sup 2} and 961.0 mGy. Conclusion: The present study results showed exposure values up to 14 times higher than those found in other foreign studies, and approximating those found for procedures in adults. Such results demonstrate excessive exposure to radiation, indicating the need for constant procedures optimization and evaluation of exposure rates. (author)

  12. Rainwater Wildlife Area Habitat Evaluation Procedures Report; A Columbia Basin Wildlife Mitigation Project.

    Energy Technology Data Exchange (ETDEWEB)

    Childs, Allen B.

    2004-01-01

    The 8,768 acre Rainwater Wildlife Area was acquired in September 1998 by the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) through an agreement with Bonneville Power Administration (BPA) to partially offset habitat losses associated with construction of the John Day and McNary hydroelectric facilities on the mainstem Columbia River. U.S. Fish and Wildlife Service (USFWS) Habitat Evaluation Procedures (HEP) were used to determine the number of habitat units credited to BPA for acquired lands. Upland and riparian forest, upland and riparian shrub, and grassland cover types are evaluated in this study. Targeted wildlife species include downy woodpecker (Picoides pubescens), black-capped chickadee (Parus atricopillus), blue grouse (Dendragapus obscurus), great blue heron (Ardea herodias), yellow warbler (Dendroica petechia), mink (Mustela vison), and Western meadowlark (Sturnella neglecta). Habitat surveys were conducted in 1998 and 1999 in accordance with published HEP protocols and included 65,300, 594m{sup 2}2 plots, and 112 one-tenth-acre plots. Between 153.3 and 7,187.46 acres were evaluated for each target wildlife mitigation species. Derived habitat suitability indices were multiplied by corresponding cover-type acreages to determine the number of habitat units for each species. The total baseline habitat units credited to BPA for the Rainwater Wildlife Area and its seven target species is 5,185.3 habitat units. Factors limiting habitat suitability are related to the direct, indirect, and cumulative effects of past livestock grazing, road construction, and timber harvest which have simplified the structure, composition, and diversity of native plant communities. Alternatives for protecting and improving habitat suitability include exclusion of livestock grazing, road de-commissioning/obliteration, reforestation and thinning, control of competing and unwanted vegetation (including noxious weeds), reestablishing displaced or reduced native

  13. Evaluation of Synergetic Anticancer Activity of Berberine and Curcumin on Different Models of A549, Hep-G2, MCF-7, Jurkat, and K562 Cell Lines

    Directory of Open Access Journals (Sweden)

    Acharya Balakrishna

    2015-01-01

    Full Text Available Ayurvedic system of medicine is using Berberis aristata and Curcuma longa herbs to treat different diseases including cancer. The study was performed to evaluate the synergetic anticancer activity of Berberine and Curcumin by estimating the inhibition of the cell proliferation by cytotoxicity assay using MTT method on specified human cell lines (A549, Hep-G2, MCF-7, Jurkat, and K562. All the cells were harvested from the culture and seeded in the 96-well assay plates at seeding density of 2.0 × 104 cells/well and were incubated for 24 hours. Test items Berberine with Curcumin (1 : 1, Curcumin 95% pure, and Berberine 95% pure were exposed at the concentrations of 1.25, 0.001, and 0.5 mg/mL, respectively, and incubated for a period of 48 hours followed by dispensing MTT solution (5 mg/mL. The cells were incubated at 37 ± 1°C for 4 hours followed by addition of DMSO for dissolving the formazan crystals and absorbance was read at 570 nm. Separate wells were prepared for positive control, controls (only medium with cells, and blank (only medium. The results had proven the synergetic anticancer activity of Berberine with Curcumin inducing cell death greater percentage of >77% when compared to pure curcumin with <54% and pure Berberine with <45% on average on all cell line models.

  14. Common HEP UNIX Environment

    Science.gov (United States)

    Taddei, Arnaud

    After it had been decided to design a common user environment for UNIX platforms among HEP laboratories, a joint project between DESY and CERN had been started. The project consists in 2 phases: 1. Provide a common user environment at shell level, 2. Provide a common user environment at graphical level (X11). Phase 1 is in production at DESY and at CERN as well as at PISA and RAL. It has been developed around the scripts originally designed at DESY Zeuthen improved and extended with a 2 months project at CERN with a contribution from DESY Hamburg. It consists of a set of files which are customizing the environment for the 6 main shells (sh, csh, ksh, bash, tcsh, zsh) on the main platforms (AIX, HP-UX, IRIX, SunOS, Solaris 2, OSF/1, ULTRIX, etc.) and it is divided at several "sociological" levels: HEP, site, machine, cluster, group of users and user with some levels which are optional. The second phase is under design and a first proposal has been published. A first version of the phase 2 exists already for AIX and Solaris, and it should be available for all other platforms, by the time of the conference. This is a major collective work between several HEP laboratories involved in the HEPiX-scripts and HEPiX-X11 working-groups.

  15. Procedures for using expert judgment to estimate human-error probabilities in nuclear power plant operations. [PWR; BWR

    Energy Technology Data Exchange (ETDEWEB)

    Seaver, D.A.; Stillwell, W.G.

    1983-03-01

    This report describes and evaluates several procedures for using expert judgment to estimate human-error probabilities (HEPs) in nuclear power plant operations. These HEPs are currently needed for several purposes, particularly for probabilistic risk assessments. Data do not exist for estimating these HEPs, so expert judgment can provide these estimates in a timely manner. Five judgmental procedures are described here: paired comparisons, ranking and rating, direct numerical estimation, indirect numerical estimation and multiattribute utility measurement. These procedures are evaluated in terms of several criteria: quality of judgments, difficulty of data collection, empirical support, acceptability, theoretical justification, and data processing. Situational constraints such as the number of experts available, the number of HEPs to be estimated, the time available, the location of the experts, and the resources available are discussed in regard to their implications for selecting a procedure for use.

  16. Parâmetros dopplervelocimétricos na avaliação da perviedade da anastomose portossistêmica intra-hepática transjugular (TIPS Dopplerflowmetric patterns for evaluation of transjugular intrahepatic portosystemic shunt patency

    Directory of Open Access Journals (Sweden)

    Antonio Sergio Zafred Marcelino

    2005-02-01

    Full Text Available A anastomose portossistêmica intra-hepática transjugular (TIPS é um procedimento intervencionista minimamente invasivo realizado pela introdução de prótese metálica auto-expansível no parênquima hepático, via transjugular. Tem por objetivo tratar as complicações da hipertensão portal, principalmente a hemorragia digestiva alta e a ascite refratária. A estenose é complicação freqüente, embora o procedimento seja eficaz e com baixo índice de insucesso. O diagnóstico precoce da estenose é de fundamental importância, pois interfere no tipo de tratamento a ser realizado e o reaparecimento dos sintomas pode ser grave. O ultra-som Doppler é então utilizado para o seguimento dos pacientes portadores do TIPS, e vários parâmetros são descritos na literatura para o diagnóstico de estenose, como: as velocidades mínima e máxima no interior da prótese, a velocidade na veia porta, o gradiente de velocidade entre dois pontos da prótese, e outros. Infelizmente não há consenso sobre qual parâmetro ou conjunto de parâmetros é mais eficaz no diagnóstico, porque os protocolos de avaliação variam de instituição para instituição. Os autores realizaram uma revisão dos parâmetros de estenose descritos na literatura e de outros aspectos de fundamental importância na compreensão do procedimento, como as indicações, as contra-indicações e a fisiopatologia da estenose.Transjugular intrahepatic portosystemic shunt (TIPS is a minimally invasive interventional procedure that consists of placement of an auto expandable metallic stent in the hepatic parenchyma via transjugular. It is used to treat the complications of portal hypertension, particularly digestive bleeding of gullet varices and refractory ascites. Although TIPS is an efficient procedure with low rate of failure some complications such as stenosis are frequent. Early diagnosis of stenosis is mandatory since it interferes with the type of treatment and the

  17. Accident Sequence Evaluation Program: Human reliability analysis procedure

    Energy Technology Data Exchange (ETDEWEB)

    Swain, A.D.

    1987-02-01

    This document presents a shortened version of the procedure, models, and data for human reliability analysis (HRA) which are presented in the Handbook of Human Reliability Analysis With emphasis on Nuclear Power Plant Applications (NUREG/CR-1278, August 1983). This shortened version was prepared and tried out as part of the Accident Sequence Evaluation Program (ASEP) funded by the US Nuclear Regulatory Commission and managed by Sandia National Laboratories. The intent of this new HRA procedure, called the ''ASEP HRA Procedure,'' is to enable systems analysts, with minimal support from experts in human reliability analysis, to make estimates of human error probabilities and other human performance characteristics which are sufficiently accurate for many probabilistic risk assessments. The ASEP HRA Procedure consists of a Pre-Accident Screening HRA, a Pre-Accident Nominal HRA, a Post-Accident Screening HRA, and a Post-Accident Nominal HRA. The procedure in this document includes changes made after tryout and evaluation of the procedure in four nuclear power plants by four different systems analysts and related personnel, including human reliability specialists. The changes consist of some additional explanatory material (including examples), and more detailed definitions of some of the terms. 42 refs.

  18. 23 CFR 669.21 - Procedure for evaluating state compliance.

    Science.gov (United States)

    2010-04-01

    ... automated file as evidence that proof of payment has been received before vehicles subject to the Federal... TRAFFIC OPERATIONS ENFORCEMENT OF HEAVY VEHICLE USE TAX § 669.21 Procedure for evaluating state compliance.... In lieu of retention of Schedule 1, states may make an appropriate entry in an automated file or on...

  19. An approach to the ethical evaluation of innovative surgical procedures.

    Science.gov (United States)

    Tan, Veronique K M; Chow, Pierce K H

    2011-01-01

    While there is an ethical obligation to improve clinical outcomes by developing better therapies, surgical innovation has largely progressed without the strict regulations required of novel pharmaceutical products. We explore the reasons why new surgical techniques are frequently introduced without the benefit of randomised controlled trials, and present an approach to the ethical evaluation of novel surgical procedures.

  20. A new tool for the evaluation of the analytical procedure: Green Analytical Procedure Index.

    Science.gov (United States)

    Płotka-Wasylka, J

    2018-05-01

    A new means for assessing analytical protocols relating to green analytical chemistry attributes has been developed. The new tool, called GAPI (Green Analytical Procedure Index), evaluates the green character of an entire analytical methodology, from sample collection to final determination, and was created using such tools as the National Environmental Methods Index (NEMI) or Analytical Eco-Scale to provide not only general but also qualitative information. In GAPI, a specific symbol with five pentagrams can be used to evaluate and quantify the environmental impact involved in each step of an analytical methodology, mainly from green through yellow to red depicting low, medium to high impact, respectively. The proposed tool was used to evaluate analytical procedures applied in the determination of biogenic amines in wine samples, and polycyclic aromatic hydrocarbon determination by EPA methods. GAPI tool not only provides an immediately perceptible perspective to the user/reader but also offers exhaustive information on evaluated procedures. Copyright © 2018 Elsevier B.V. All rights reserved.

  1. A virtual reality simulator for teaching and evaluating dental procedures.

    Science.gov (United States)

    Rhienmora, P; Haddawy, P; Khanal, P; Suebnukarn, S; Dailey, M N

    2010-01-01

    We present a dental training system with a haptic interface that allows dental students or experts to practice dental procedures in a virtual environment. The simulator is able to monitor and classify the performance of an operator into novice or expert categories. The intelligent training module allows a student to simultaneously and proactively follow the correct dental procedures demonstrated by an intelligent tutor. The virtual reality (VR) simulator simulates the tooth preparation procedure both graphically and haptically, using a video display and haptic device. We evaluated the performance of users using hidden Markov models (HMMs) incorporating various data collected by the simulator. We implemented an intelligent training module which is able to record and replay the procedure that was performed by an expert and allows students to follow the correct steps and apply force proactively by themselves while reproducing the procedure. We find that the level of graphics and haptics fidelity is acceptable as evaluated by dentists. The accuracy of the objective performance assessment using HMMs is encouraging with 100 percent accuracy. The simulator can simulate realistic tooth surface exploration and cutting. The accuracy of automatic performance assessment system using HMMs is also acceptable on relatively small data sets. The intelligent training allows skill transfer in a proactive manner which is an advantage over the passive method in a traditional training. We will soon conduct experiments with more participants and implement a variety of training strategies.

  2. HEP in Greek Classes

    Science.gov (United States)

    Fassouliotis, Dimitris; Kourkoumelis, Christine; Vourakis, Stylianos

    2017-03-01

    The HEP Inquiry learning resources created over the last four years by the European outreach projects are reviewed. The resources are mostly addressed to high school students and the purpose is to ignite their interest on science. In addition, at the University of Athens for the last four years we have been using the HYPATIA online event analysis tool as a lab course for fourth year undergraduate physics students, majoring in HEP. Each year 40-50 students highly appreciated the course, since they get a direct involvement in the actual toplevel research. Up to now, the course was limited to visual inspection of a few tens of ATLAS events. Recently we have enriched the course with additional analysis exercises, which involve large samples of events. The students, through a user friendly interface can analyze the samples (both signal and background ones) and optimize the cut selection in order to search for the Higgs decay H □ 4 leptons. Recently ATLAS released 1/fb of data, so starting now the students analyse real data.

  3. Evaluation of the histopathological hepatic lesions and opportunistic agents in Brazilian HIV patients Avaliação das alterações histológicas hepáticas e infecções oportunistas em pacientes brasileiros infectados pelo HIV

    Directory of Open Access Journals (Sweden)

    Graziella Hanna Pereira

    2010-02-01

    Full Text Available INTRODUCTION: to evaluated the type histopathological hepatic lesions and opportunistic agents in Brazilian HIV-infected patients. METHODS: we examined 52 percutaneous liver biopsies of 50 HIV-infected patients who had at least two of the following conditions: fever of unknown origin, unexplained severe emaciation, hepatomegaly or abnormal liver chemistry. The specimens were cultured for mycobacteria and fungi and stained by standard procedures. RESULTS: reactive patterns, granulomatous hepatitis and chronic active hepatitis were verified in 28 (54%, 11 (21% and 8 (15% of the patients respectively. Opportunistic infections were diagnosed in 18 (36% patients: mycobacteria in 12 (24%, Cryptococcus neoformans in 5 (10% patients and mycobacteria and yeast was isolated from the same liver fragment in one patient. CONCLUSIONS: mycobacteriosis was the most common opportunistic infection and liver tissue culture is an important method to detect opportunistic agents, even in the absence of histological lesions.INTRODUÇÃO: avaliar os tipos de lesões histopatológicas e infecções oportunistas de Brasileiros infectados pelo HIV. MÉTODOS: Foram analisadas 52 biópsias hepáticas percutâneas de 50 pacientes que apresentavam pelo menos duas das alterações: febre de origem indeterminada, emagrecimento inexplicado, hepatomegalia ou anormalidades na bioquímica hepática. O fragmento de tecido hepático foi submetido a histopatologia por métodos habituais e cultura para micobacteria e fungo. RESULTADOS: padrão reacional, hepatite granulomatosa e hepatite crônica ativa foram encontrados em 28 (54%, 11 (21% e 8 (15% dos pacientes respectivamente. Infecções oportunistas foram diagnosticadas em 18 (36% dos pacientes: micobacteria em 12 (24%, Cryptococcus neoformans em 5 (10% pacientes e micobacteria e fungo foram isolados no mesmo fragmento em um paciente. CONCLUSÕES: micobacteriose foi a infecção oportunista mais comum e a cultura de tecido hep

  4. New procedures to evaluate visually lossless compression for display systems

    Science.gov (United States)

    Stolitzka, Dale F.; Schelkens, Peter; Bruylants, Tim

    2017-09-01

    Visually lossless image coding in isochronous display streaming or plesiochronous networks reduces link complexity and power consumption and increases available link bandwidth. A new set of codecs developed within the last four years promise a new level of coding quality, but require new techniques that are sufficiently sensitive to the small artifacts or color variations induced by this new breed of codecs. This paper begins with a summary of the new ISO/IEC 29170-2, a procedure for evaluation of lossless coding and reports the new work by JPEG to extend the procedure in two important ways, for HDR content and for evaluating the differences between still images, panning images and image sequences. ISO/IEC 29170-2 relies on processing test images through a well-defined process chain for subjective, forced-choice psychophysical experiments. The procedure sets an acceptable quality level equal to one just noticeable difference. Traditional image and video coding evaluation techniques, such as, those used for television evaluation have not proven sufficiently sensitive to the small artifacts that may be induced by this breed of codecs. In 2015, JPEG received new requirements to expand evaluation of visually lossless coding for high dynamic range images, slowly moving images, i.e., panning, and image sequences. These requirements are the basis for new amendments of the ISO/IEC 29170-2 procedures described in this paper. These amendments promise to be highly useful for the new content in television and cinema mezzanine networks. The amendments passed the final ballot in April 2017 and are on track to be published in 2018.

  5. Cytotoxic Activity of Origanum Vulgare L. on Hepatocellular Carcinoma cell Line HepG2 and Evaluation of its Biological Activity

    Directory of Open Access Journals (Sweden)

    Hazem S. Elshafie

    2017-08-01

    Full Text Available The potential of plant essential oils (EOs in anticancer treatment has recently received many research efforts to overcome the development of multidrug resistance and their negative side effects. The aims of the current research are to study (i the cytotoxic effect of the crude EO extracted from Origanum vulgare subsp hirtum and its main constituents (carvacrol, thymol, citral and limonene on hepatocarcinoma HepG2 and healthy human renal cells HEK293; (ii the antibacterial and phytotoxic activities of the above EO and its main constituents. Results showed that cell viability percentage of treated HepG2 by EO and its main constituents was significantly decreased when compared to untreated cells. The calculated inhibition concentration (IC50 values for HepG2 were lower than healthy renal cells, indicating the sort of selectivity of the studied substances. Citral is not potentially recommended as an anticancer therapeutic agent, since there are no significant differences between IC50 values against both tested cell lines. Results showed also that oregano EO and its main constituents have a significant antibacterial activity and a moderate phytotoxic effect. The current research verified that oregano EO and its main constituents could be potentially utilized as anticancer therapeutic agents.

  6. HEP technologies to address medical imaging challenges

    CERN Multimedia

    CERN. Geneva

    2016-01-01

    Developments in detector technologies aimed at solving challenges in present and future CERN experiments, particularly at the LHC, have triggered exceptional advances in the performance of medical imaging devices, allowing for a spectacular progress in in-vivo molecular imaging procedures, which are opening the way for tailored therapies of major diseases. This talk will briefly review the recent history of this prime example of technology transfer from HEP experiments to society, will describe the technical challenges being addressed by some ongoing projects, and will present a few new ideas for further developments and their foreseeable impact.

  7. Evaluation of Computer-Based Procedure System Prototype

    Energy Technology Data Exchange (ETDEWEB)

    Johanna Oxstrand; Katya Le Blanc; Seth Hays

    2012-09-01

    relevant for the task and situation at hand, which has potential consequences of taking up valuable time when operators must be responding to the situation, and potentially leading operators down an incorrect response path. Other challenges related to PBPs are the management of multiple procedures, place-keeping, finding the correct procedure for the task at hand, and relying on other sources of additional information to ensure a functional and accurate understanding of the current plant status (Converse, 1995; Fink, Killian, Hanes, & Naser, 2009; Le Blanc & Oxstrand, 2012). The main focus of this report is to describe the research activities conducted to address the remaining two objectives; Develop a prototype CBP system based on requirements identified and Evaluate the CBP prototype. The emphasis will be on the evaluation of an initial CBP prototype in at a Nuclear Power Plant.

  8. Evaluating Virtual Reality Mood Induction Procedures with Portable EEG Devices

    OpenAIRE

    Rodriguez Ortega, Alejandro; Rey Solaz, Beatriz; Alcañiz Raya, Mariano Luis

    2013-01-01

    Virtual Environments (VEs) have been used as mood induction procedures. In this context, it is necessary to have instruments to analyze the emotional state during VE exposure. Objective techniques such as EEG should be evaluated for this purpose. The aim in this work was to study the changes in the brain activity with a portable EEG device during a negative mood induction based on a VE. A virtual park was used to induce a negative mood (sadness) in ten participants. Changes in the brain activ...

  9. Electromagnetic Nondestructive Evaluation of Tubes using Data Mining Procedure

    Science.gov (United States)

    Savin, A.; Iftimie, N.; Vizureanu, P.; Steigmann, R.; Dobrescu, G. S.

    2017-06-01

    The fundamental issues in nondestructive evaluation consists in the identification of events corresponding to the flaws which can appear in the examined object and their extraction from noises. This is usually done by comparison with pre-established thresholds, experimentally determined by using standard samples or in the basis of the solution of the forward problem and simulations. This paper presents the features extraction using data mining procedure in the case of tubes from steam generators having different flaws. The data mining is carried on using simulated models in CIVA 9 and experimental data gathered using an inner differential sensor developed in this purpose.

  10. A procedure to evaluate environmental rehabilitation in limestone quarries.

    Science.gov (United States)

    Neri, Ana Claudia; Sánchez, Luis Enrique

    2010-11-01

    A procedure to evaluate mine rehabilitation practices during the operational phase was developed and validated. It is based on a comparison of actually observed or documented practices with internationally recommended best practices (BP). A set of 150 BP statements was derived from international guides in order to establish the benchmark. The statements are arranged in six rehabilitation programs under three categories: (1) planning (2) operational and (3) management, corresponding to the adoption of the plan-do-check-act management systems model to mine rehabilitation. The procedure consists of (i) performing technical inspections guided by a series of field forms containing BP statements; (ii) classifying evidences in five categories; and (iii) calculating conformity indexes and levels. For testing and calibration purposes, the procedure was applied to nine limestone quarries and conformity indexes were calculated for the rehabilitation programs in each quarry. Most quarries featured poor planning practices, operational practices reached high conformity levels in 50% of the cases and management practices scored moderate conformity. Despite all quarries being ISO 14001 certified, their management systems pay low attention to issues pertaining to land rehabilitation and biodiversity. The best results were achieved by a quarry whose expansion was recently submitted to the environmental impact assessment process, suggesting that public scrutiny may play a positive role in enhancing rehabilitation practices. Conformity indexes and levels can be used to chart the evolution of rehabilitation practices at regular intervals, to establish corporate goals and for communication with stakeholders. Copyright 2010 Elsevier Ltd. All rights reserved.

  11. In vitro Evaluation of Cytotoxic Activities of Essential Oil from Moringa oleifera Seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 Cell Lines.

    Science.gov (United States)

    Elsayed, Elsayed Ahmed; Sharaf-Eldin, Mahmoud A; Wadaan, Mohammad

    2015-01-01

    Moringa oleifera Lam. (Moringaceae) is widely consumed in tropical and subtropical regions for their valuable nutritional and medicinal characteristics. Recently, extensive research has been conducted on leaf extracts of M. oleifera to evaluate their potential cytotoxic effects. However, with the exception of antimicrobial and antioxidant activities, little information is present on the cytotoxic activity of the essential oil obtained from M. oleifera seeds. Therefore, the present investigation was designed to investigate the potential cytotoxic activity of seed essential oil obtained from M. oleifera on HeLa, HepG2, MCF-7, CACO-2 and L929 cell lines. The different cell lines were subjected to increasing oil concentrations ranging from 0.15 to 1 mg/mL for 24h, and the cytotoxicity was assessed using MTT assay. All treated cell lines showed a significant reduction in cell viability in response to the increasing oil concentration. Moreover, the reduction depended on the cell line as well as the oil concentration applied. Additionally, HeLa cells were the most affected cells followed by HepG2, MCF-7, L929 and CACO-2, where the percentages of cell toxicity recorded were 76.1, 65.1, 59.5, 57.0 and 49.7%, respectively. Furthermore, the IC50 values obtained for MCF-7, HeLa and HepG2 cells were 226.1, 422.8 and 751.9 μg/mL, respectively. Conclusively, the present investigation provides preliminary results which suggest that seed essential oil from M. oleifera has potent cytotoxic activities against cancer cell lines.

  12. MR angiography of hemodialysis access fistula. Evaluation before PTA procedure

    Energy Technology Data Exchange (ETDEWEB)

    Morimoto, Atsuko; Yoshimura, Hirohide; Hosokawa, Chisa; Yoshida, Toshiko [Yodogawa Christian Hospital, Osaka (Japan)

    2000-08-01

    We evaluated the feasibility of MR angiography for hemodialysis fistula. Eleven patients with suspected stenosis or occlusion of an autogenous hemodialysis fistula in the forearm underwent MRA. MRA was obtained using both the 3D-PC method and 3D gadolinium contrast-enhanced (CE) method with a knee coil. The two methods were compared with DSA in seven patients. CE-MRA clearly demonstrated the entire dialysis fistula. However, signals of the stenotic area are effaced in PC-MRA. The diameter of the vessels evaluated in the CE-MRA was well correlated with angiography. CE-MRA demonstrated smaller collateral vessels more clearly than PC-MRA. CE-MRA provided useful information before the PTA procedure. (author)

  13. Evaluation of Novel (64)Cu-Labeled Theranostic Gadolinium-Based Nanoprobes in HepG2 Tumor-Bearing Nude Mice.

    Science.gov (United States)

    Hu, Pengcheng; Cheng, Dengfeng; Huang, Tao; Banizs, Anna B; Xiao, Jie; Liu, Guobing; Chen, Quan; Wang, Yuenan; He, Jiang; Shi, Hongcheng

    2017-09-06

    Radiation therapy of liver cancer is limited by low tolerance of the liver to radiation. Radiosensitizers can effectively reduce the required radiation dose. AGuIX nanoparticles are small, multifunctional gadolinium-based nanoparticles that can carry radioisotopes or fluorescent markers for single-photon emission computed tomography (SPECT), positron emission tomography (PET), fluorescence imaging, and even multimodality imaging. In addition, due to the high atomic number of gadolinium, it can also serve as a tumor radiation sensitizer. It is critical to define the biodistribution and pharmacokinetics of these gadolinium-based nanoparticles to quantitate the magnitude and duration of their retention within the tumor microenvironment during radiotherapy. Therefore, in this study, we successfully labeled AGuIX with (64)Cu through the convenient built-in chelator. The biodistribution studies indicated that the radiotracer (64)Cu-AGuIX accumulates to high levels in the HepG2 xenograft of nude mice, suggesting that it would be a potential theranostic nanoprobe for image-guided radiotherapy in HCC. We also used a transmission electron microscope to confirm AGuIX uptake in the HepG2 cells. In radiation therapy studies, a decrease in (18)F-FDG uptake was observed in the xenografts of the nude mice irradiated with AGuIX, which was injected 1 h before. These results provide proof-of-concept that AGuIX can be used as a theranostic radiosensitizer for PET imaging to guide radiotherapy for liver cancer.

  14. Evaluation of Novel 64Cu-Labeled Theranostic Gadolinium-Based Nanoprobes in HepG2 Tumor-Bearing Nude Mice

    Science.gov (United States)

    Hu, Pengcheng; Cheng, Dengfeng; Huang, Tao; Banizs, Anna B.; Xiao, Jie; Liu, Guobing; Chen, Quan; Wang, Yuenan; He, Jiang; Shi, Hongcheng

    2017-09-01

    Radiation therapy of liver cancer is limited by low tolerance of the liver to radiation. Radiosensitizers can effectively reduce the required radiation dose. AGuIX nanoparticles are small, multifunctional gadolinium-based nanoparticles that can carry radioisotopes or fluorescent markers for single-photon emission computed tomography (SPECT), positron emission tomography (PET), fluorescence imaging, and even multimodality imaging. In addition, due to the high atomic number of gadolinium, it can also serve as a tumor radiation sensitizer. It is critical to define the biodistribution and pharmacokinetics of these gadolinium-based nanoparticles to quantitate the magnitude and duration of their retention within the tumor microenvironment during radiotherapy. Therefore, in this study, we successfully labeled AGuIX with 64Cu through the convenient built-in chelator. The biodistribution studies indicated that the radiotracer 64Cu-AGuIX accumulates to high levels in the HepG2 xenograft of nude mice, suggesting that it would be a potential theranostic nanoprobe for image-guided radiotherapy in HCC. We also used a transmission electron microscope to confirm AGuIX uptake in the HepG2 cells. In radiation therapy studies, a decrease in 18F-FDG uptake was observed in the xenografts of the nude mice irradiated with AGuIX, which was injected 1 h before. These results provide proof-of-concept that AGuIX can be used as a theranostic radiosensitizer for PET imaging to guide radiotherapy for liver cancer.

  15. Comparative cytotoxicity of dolomite nanoparticles in human larynx HEp2 and liver HepG2 cells.

    Science.gov (United States)

    Ahamed, Maqusood; Alhadlaq, Hisham A; Ahmad, Javed; Siddiqui, Maqsood A; Khan, Shams T; Musarrat, Javed; Al-Khedhairy, Abdulaziz A

    2015-06-01

    Dolomite is a natural mineral of great industrial and commercial importance. With the advent of nanotechnology, natural minerals including dolomite in the form of nanoparticles (NPs) are being utilized in various applications to improve the quality of products. However, safety or toxicity information of dolomite NPs is largely lacking. This study evaluated the cytotoxicity of dolomite NPs in two widely used in vitro cell culture models: human airway epithelial (HEp2) and human liver (HepG2) cells. Concentration-dependent decreased cell viability and damaged cell membrane integrity revealed the cytotoxicity of dolomite NPs. We further observed that dolomite NPs induce oxidative stress in a concentration-dependent manner, as indicated by depletion of glutathione and induction of reactive oxygen species (ROS) and lipid peroxidation. Quantitative real-time PCR data demonstrated that the mRNA level of tumor suppressor gene p53 and apoptotic genes (bax, CASP3 and CASP9) were up-regulated whereas the anti-apoptotic gene bcl-2 was down-regulated in HEp2 and HepG2 cells exposed to dolomite NPs. Moreover, the activity of apoptotic enzymes (caspase-3 and caspase-9) was also higher in both kinds of cells treated with dolomite NPs. It is also worth mentioning that HEp2 cells seem to be marginally more susceptible to dolomite NPs exposure than HepG2 cells. Cytotoxicity induced by dolomite NPs was efficiently prevented by N-acetyl cysteine treatment, which suggests that oxidative stress is primarily responsible for the cytotoxicity of dolomite NPs in both HEp2 and HepG2 cells. Toxicity mechanisms of dolomite NPs warrant further investigations at the in vivo level. Copyright © 2015 John Wiley & Sons, Ltd.

  16. An Extensive QFD and Evaluation Procedure for Innovative Design

    Directory of Open Access Journals (Sweden)

    Chang-Tzuoh Wu

    2013-01-01

    Full Text Available In this paper, a systematic innovation procedure has been developed based on quality function deployment and extension method. Besides, the evaluation method for design decision making has also been discussed and developed. The major procedure of QFD is to identify the customers’ needs for the product and then convert into appropriate technical measures to fulfill the needs based on the company’s competitive priorities. The priorities of product characteristics can be obtained by translating important technical measures. According to their characteristics, the prior engineering parameters will be identified and selected as the key requirements to redesign. This paper will focus on the integration of QFD and extension method. With the help of “matter element theory and extension method,” customer requirements (CRs can be transferred into product design attributes more comprehensively and deeply. According to the idea of loss function of Taguchi quality design method, the criteria for design decision making have also been developed in this paper. An innovative design case, bicycle, successfully demonstrates that the proposed design process is feasible and efficient.

  17. Evaluating virtual reality mood induction procedures with portable EEG devices.

    Science.gov (United States)

    Rodríguez, Alejandro; Rey, Beatriz; Alcañiz, Mariano

    2013-01-01

    Virtual Environments (VEs) have been used as mood induction procedures. In this context, it is necessary to have instruments to analyze the emotional state during VE exposure. Objective techniques such as EEG should be evaluated for this purpose. The aim in this work was to study the changes in the brain activity with a portable EEG device during a negative mood induction based on a VE. A virtual park was used to induce a negative mood (sadness) in ten participants. Changes in the brain activity of subjects were compared between two moments (before and after emotional induction). Obtained results were in accordance with previous scientific literature regarding frontal EEG asymmetry, which supports the possibility of using the portable EEG as a reliable instrument to measure emotions in VE.

  18. Evaluation of antioxidant potential of leaves of Leonotis nepetifolia and its inhibitory effect on MCF7 and Hep2 cancer cell lines

    Directory of Open Access Journals (Sweden)

    Usharani Veerabadran

    2013-04-01

    Full Text Available Objective: To investigate the antioxidant and antiproliferative potential of Leonotis nepetifolia (L. nepetifolia leaves. Methods: The leaves of L. nepetifolia were subjected to extraction using three different solvents and the antioxidant potential of those extracts were tested by using various in vitro assays. Further, the best screened extract was analyzed for its phytochemical profile by both qualitative and quantitative assays. In order to determine its anti-proliferative activity, the best screened extract was treated with breast and laryngeal cancer cell lines such as MCF-7 cells and Hep2 cells, respectively. The cytotoxicity of the extract was also studied using MTT assay. The inhibitory effect of the extract of leaves of L. nepetifolia on the selected cell-line DNA was determined by DNA fragmentation assay. Also, the extract was subjected to TLC and bioautography analysis. Results: The DPPH assay showed methanol extract of L. nepetifolia leaves to be more significant in scavenging free radicals with inhibition percentage of 60.57%. From the data obtained, the methanol extract proved to be significant in all anti-oxidant assays and this effect was well comparable with the standard used in the study. The predominant phytochemicals such as phenols and flavonoids were further quantified as 0.107% and 0.089%. The cytotoxicity assay showed that the cell viability increased with increasing concentration of methanol extract. In addition, the extract caused dose dependent damage to the cancer cell lines MCF-7 and Hep2. Conclusions: Our study suggests that the leaves of L. nepetifolia were significant in scavenging free radicals and causing damage to proliferative cells. Further mechanistic studies would help in proving the efficiency of the selected plant under in vivo conditions.

  19. Evaluation of antioxidant potential of leaves of Leonotis nepetifolia and its inhibitory effect on MCF7 and Hep2 cancer cell lines

    Science.gov (United States)

    Veerabadran, Usharani; Venkatraman, Anuradha; Souprayane, Aroumougame; Narayanasamy, Mathivanan; Perumal, Dhanalakshmi; Elumalai, Sagadevan; Sivalingam, Sindhu; Devaraj, Vadivelu; Perumal, Arumugam

    2013-01-01

    Objective To investigate the antioxidant and antiproliferative potential of Leonotis nepetifolia (L. nepetifolia) leaves. Methods The leaves of L. nepetifolia were subjected to extraction using three different solvents and the antioxidant potential of those extracts were tested by using various in vitro assays. Further, the best screened extract was analyzed for its phytochemical profile by both qualitative and quantitative assays. In order to determine its anti-proliferative activity, the best screened extract was treated with breast and laryngeal cancer cell lines such as MCF-7 cells and Hep2 cells, respectively. The cytotoxicity of the extract was also studied using MTT assay. The inhibitory effect of the extract of leaves of L. nepetifolia on the selected cell-line DNA was determined by DNA fragmentation assay. Also, the extract was subjected to TLC and bioautography analysis. Results The DPPH assay showed methanol extract of L. nepetifolia leaves to be more significant in scavenging free radicals with inhibition percentage of 60.57%. From the data obtained, the methanol extract proved to be significant in all anti-oxidant assays and this effect was well comparable with the standard used in the study. The predominant phytochemicals such as phenols and flavonoids were further quantified as 0.107% and 0.089%. The cytotoxicity assay showed that the cell viability increased with increasing concentration of methanol extract. In addition, the extract caused dose dependent damage to the cancer cell lines MCF-7 and Hep2. Conclusions Our study suggests that the leaves of L. nepetifolia were significant in scavenging free radicals and causing damage to proliferative cells. Further mechanistic studies would help in proving the efficiency of the selected plant under in vivo conditions.

  20. Estudio prospectivo sobre la utilidad de la ecografía de control tras la realización de pruebas invasivas hepáticas: biopsia hepática y punción aspiración con aguja fina (PAAF A prospective study about the usefulness of ultrasonographic monitoring after invasive liver procedures: liver biopsy and fine-needle aspiration (FNA

    Directory of Open Access Journals (Sweden)

    E. Carrera Alonso

    2007-03-01

    Full Text Available Objetivo: establecer si es necesario realizar una ecografía de control a todos los pacientes sometidos a una biopsia hepática o una punción aspiración con aguja fina, para detectar posibles complicaciones con o sin repercusión clínica. Material y métodos: tras la realización de una biopsia hepática o una punción aspiración con aguja fina según el protocolo habitual, se mantiene al paciente en observación durante 24 horas, realizándose en ese momento una ecografía a todos los pacientes aunque no presenten datos clínicos de complicación. Resultados: se llevaron a cabo 298 biopsias hepáticas y 98 punciones mediante aguja fina. Presentaron complicaciones un total de 37 pacientes (9,34%, de las cuales 36 (9,09% fueron complicaciones menores en forma de dolor, síncope vasovagal o hemorragia leve y 1 (0,25% complicación mayor en forma de hemorragia grave. De las 396 exploraciones tan sólo uno de los casos presentó una complicación detectada en la ecografía (hematoma intraparenquimatoso encontrándose asintomático. Conclusiones: la baja incidencia de complicaciones, que cursan de forma asintomática, y la buena evolución de las mismas hacen poco rentable la realización de ecografía de control tras la realización de dichas técnicas diagnósticas, siendo necesaria tan sólo en el caso de sospecha clínica de complicación.Objective: to determine the need to perform ultrasound scans to all patients after liver biopsy or fine-needle aspiration (FNA in order to detect complications with or without symptoms. Material and methods: after liver biopsy or FNA using a regular protocol the patient is observed for 24 hours at the hospital, and all patients undergo an abdominal sonography at that time even in the absence of evident complications. Results: 298 liver biopsies and 98 FNAs were performed. There were complications in 37 patients (9.34%: 36 (9.09% were minor complications such as pain, vasovagal episodes, or small bleeding

  1. Networking: the view from HEP

    Science.gov (United States)

    McKee, Shawn

    2017-10-01

    Networks have played a critical role in high-energy physics (HEP), enabling us to access and effectively utilize globally distributed resources to meet the needs of our physicists. National and global-scale collaborations that characterize HEP would not be feasible without ubiquitous capable networks. Because of their importance in enabling our grid computing infrastructure many physicists have taken leading roles in research and education (R&E) networking, participating in, and even convening, network related meetings and research programs with the broader networking community worldwide. This has led to HEP benefiting from excellent global networking capabilities for little to no direct cost. However, as other science domains ramp-up their need for similar networking it becomes less clear that this situation will continue unchanged. This paper will briefly discuss the history of networking in HEP, the current activities and challenges we are facing, and try to provide some understanding of where networking may be going in the next 5 to 10 years.

  2. PROCEDURE FOR ANALYSIS AND EVALUATION OF MARKET POSITION PRODUCTION ORGANIZATION

    Directory of Open Access Journals (Sweden)

    A. N. Polozova

    2014-01-01

    Full Text Available Summary. Methodical procedures economic monitoring market position of industrial organization, particularly those relating to food production, including the 5 elements: matrix «component of business processes», matrix «materiality – efficiency», matrix «materiality – relevant», matrix emption and hindering factors matrix operation scenarios. Substantiated components assess the strengths and weaknesses of the business activities of organizations that characterize the state of internal business environment on the elements: production, organization, personnel, finance, marketing. The advantages of the matrix «materiality – relevance» consisting of 2 materiality level - high and low, and 3 directions relevance – «no change», «gain importance in the future», «lose importance in the future». Presented the contents of the matrix «scenarios functioning of the organization», involving 6 attribute levels, 10 classes of scenarios, 19 activities, including an optimistic and pessimistic. The evaluation of primary classes of scenarios, characterized by the properties of «development», «dynamic equilibrium», «quality improvement», «competitiveness», «favorable realization of opportunities», «competition resistance».

  3. Overview of the JPEG XS objective evaluation procedures

    Science.gov (United States)

    Willème, Alexandre; Richter, Thomas; Rosewarne, Chris; Macq, Benoit

    2017-09-01

    JPEG XS is a standardization activity conducted by the Joint Photographic Experts Group (JPEG), formally known as ISO/IEC SC29 WG1 group that aims at standardizing a low-latency, lightweight and visually lossless video compression scheme. This codec is intended to be used in applications where image sequences would otherwise be transmitted or stored in uncompressed form, such as in live production (through SDI or IP transport), display links, or frame buffers. Support for compression ratios ranging from 2:1 to 6:1 allows significant bandwidth and power reduction for signal propagation. This paper describes the objective quality assessment procedures conducted as part of the JPEG XS standardization activity. Firstly, this paper discusses the objective part of the experiments that led to the technology selection during the 73th WG1 meeting in late 2016. This assessment consists of PSNR measurements after a single and multiple compression decompression cycles at various compression ratios. After this assessment phase, two proposals among the six responses to the CfP were selected and merged to form the first JPEG XS test model (XSM). Later, this paper describes the core experiments (CEs) conducted so far on the XSM. These experiments are intended to evaluate its performance in more challenging scenarios, such as insertion of picture overlays, robustness to frame editing, assess the impact of the different algorithmic choices, and also to measure the XSM performance using the HDR VDP metric.

  4. Evaluation of vertical profiles to design continuous descent approach procedure

    Science.gov (United States)

    Pradeep, Priyank

    The current research focuses on predictability, variability and operational feasibility aspect of Continuous Descent Approach (CDA), which is among the key concepts of the Next Generation Air Transportation System (NextGen). The idle-thrust CDA is a fuel economical, noise and emission abatement procedure, but requires increased separation to accommodate for variability and uncertainties in vertical and speed profiles of arriving aircraft. Although a considerable amount of researches have been devoted to the estimation of potential benefits of the CDA, only few have attempted to explain the predictability, variability and operational feasibility aspect of CDA. The analytical equations derived using flight dynamics and Base of Aircraft and Data (BADA) Total Energy Model (TEM) in this research gives insight into dependency of vertical profile of CDA on various factors like wind speed and gradient, weight, aircraft type and configuration, thrust settings, atmospheric factors (deviation from ISA (DISA), pressure and density of the air) and descent speed profile. Application of the derived equations to idle-thrust CDA gives an insight into sensitivity of its vertical profile to multiple factors. This suggests fixed geometric flight path angle (FPA) CDA has higher degree of predictability and lesser variability at the cost of non-idle and low thrust engine settings. However, with optimized design this impact can be overall minimized. The CDA simulations were performed using Future ATM Concept Evaluation Tool (FACET) based on radar-track and aircraft type data (BADA) of the real air-traffic to some of the busiest airports in the USA (ATL, SFO and New York Metroplex (JFK, EWR and LGA)). The statistical analysis of the vertical profiles of CDA shows 1) mean geometric FPAs derived from various simulated vertical profiles are consistently shallower than 3° glideslope angle and 2) high level of variability in vertical profiles of idle-thrust CDA even in absence of

  5. In vitro evaluation of silver nanoparticles cytotoxicity on Hepatic cancer (Hep-G2) cell line and their antioxidant activity: Green approach for fabrication and application.

    Science.gov (United States)

    Kumar, Brajesh; Smita, Kumari; Seqqat, Rachid; Benalcazar, Karen; Grijalva, Marcelo; Cumbal, Luis

    2016-06-01

    In this article, biosynthesis of silver nanoparticles (AgNPs) using Andean Mora (Rubus glaucus Benth.) leaf has been reported. Different analytical techniques including UV-vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM) and X-ray diffraction (XRD) were used for the characterization of AgNPs. The initial appearance of color change with the intense surface plasmon resonance (SPR) bands around 440-455 in UV-visible spectra revealing the formation of AgNPs. The TEM image showed the AgNPs to be anisotropic, quasi-spherical in shape with sizes in the range of 12-50nm. On the other hand, XRD studies revealed the formation of face-centered cubic structure for AgNPs. The surface modified AgNPs showed no cytotoxicity at the concentration ranging from 0.01μM to 1.0μM on the Hepatic cancer (Hep-G2) cell line and observed antioxidant efficacy >70% at the concentration 0.05mM/0.20mL against 1, 1-diphenyl-2-picrylhydrazyl. From the results obtained it is suggested that AgNPs could be used effectively in future drug delivery systems and other biomedical concerns. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. A decision support system-based procedure for evaluation and ...

    Indian Academy of Sciences (India)

    institutional and governance structures, habitats and species diversity (Benoit and Comeau 2005). This poses a fundamental barrier to the develop- ment, adoption and implementation of a common procedure. Herein, a Protected Area Sustainability Eval- uation and Monitoring (PASEM) procedure for the Mediterranean is ...

  7. A Comparative Evaluation of Three Relaxation Training Procedures.

    Science.gov (United States)

    Brandon, Jeffrey E.

    Comparison was made between the effectiveness of three relaxation training procedures: (1) Behavioral Relaxation Training, which consisted of training in relaxing specific parts of the body and controlling breathing; (2) Meditation (based on Benson's procedure for eliciting the relaxation response); and (3) Seashore Sounds "Attention Focusing,"…

  8. Evaluating vowel normalisation procedures: A case study on ...

    African Journals Online (AJOL)

    In the present article, eight such procedures are tested on a large data-set of Southern Sotho vowels (4 434 tokens), as produced by twelve speakers (six of each gender and balanced as to age and locality). We concentrated on the examination of two types of normalisation procedures: firstly, the vowel-extrinsic class of ...

  9. New measuring and evaluation procedures for Tsunami Early Warning

    Science.gov (United States)

    Muench, U.; Lauterjung, J.; Rudloff, A.; Gitews-Project Team

    2009-04-01

    The Tsunami Early Warning System for the Indian Ocean just recently went into operation in Indonesia. The different sensor stations have, for the most part, been installed and now deliver respective data either online or upon request to the Warning Centre in Jakarta. Before March 2010, however, the interaction between the different component parts must be improved and optimized, personnel needs to be trained and eventual problems in the daily operation have to be dealt with. This current system differs from previous Tsunami Warning Systems through the application of modern scientific methods and technologies. New procedures for the fast and reliable determination of strong earthquakes, the modelling of tsunamis and the assessment of the situation have been implemented in the Warning System. In particular, the direct incorporation of a broad variety of different sensors provides for information from a number of sources thus resulting in a stable system and minimizing breakdowns. The system includes a seismological network, together with GPS stations and a network of GPS buoys additionally equipped with ocean bottom pressure sensors and a tide gauge network. The warning system is designed in an open and modular structure based on the most recent developments and standards of information technology. Therefore, the system can easily integrate additional sensor components to be used in other regions such as the Mediterranean Sea and/or for different purposes e.g. storm tides. Up to now the German Project Group has been cooperating in the Indian Ocean region with Sri Lanka, the Maldives, Yemen, Tanzania and Kenya to build up equipment primarily for seismological monitoring and data evaluation. Close ties have also been established with Australia, South Africa and India for the real-time exchange mainly of seismological, but also of sea level data.

  10. Procedures for evaluating pork carcass and cut composition

    Energy Technology Data Exchange (ETDEWEB)

    Siemens, A.L.H.

    1989-01-01

    Five studies were completed to investigate various production and evaluation procedures related to pork carcass composition and meat quality. A comparison of market hog characteristics of pigs selected by feeder pig frame size or current USDA feeder pig standards was made. In general, feeder pig frame size did differentiate between carcass skeletal traits (i.e., carcass length, radius length). However, frame sizing did not improve on current feeder pig grades in discriminating between carcass composition characteristics. Liquid scintillation of potassium-40 was used to estimate pork carcass composition of 124 boars barrows and gilts, ranging from 23 to 114 kg live weight. Pigs were counted live, slaughtered and one side of the carcass was counted. The side was then ground and sampled for percent protein, fat and moisture. Carcass weight and {sup 40}K determined potassium of the carcass explain more of the variation in carcass composition than live animal traits. Carcass measurements were used to determine value and percentages of fat standardized lean, protein, fat and moisture in the carcass using 265 barrow and gilt carcasses. In a separate study, belly composition was estimated from carcass and belly parameters (n = 338). Ribbed carcasses measurements were almost always superior to unribbed carcass measurements when estimating carcass or belly composition. Tenth rib fat depth was the most useful single variable for predicting belly fat, protein, moisture and lean. Some precision and accuracy were lost when using parameters from unribbed carcasses to estimate carcass or belly composition as compared to including parameters from ribbed carcasses. The sensory and nutritive value of cooked pork center loin chops and roasts were investigated. Levels of fat cover and internal temperature did not greatly affect cholesterol content.

  11. Evaluation of toxicological biomarkers in secreted proteins of HepG2 cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin and their expressions in the plasma of rats and incineration workers.

    Science.gov (United States)

    Phark, Sohee; Park, So-Young; Chang, Yoon-Seok; Choi, Seonyoung; Lim, Ji-youn; Kim, Yoonjin; Seo, Jong Bok; Jung, Woon-Won; Sul, Donggeun

    2016-05-01

    Toxicological biomarkers of 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) were investigated in proteins secreted by HepG2 cells and their expression levels were determined in the plasma of rats exposed to 2,3,7,8-TCDD and in the plasma of incineration workers exposed to dioxins. HepG2 cells were treated with various concentrations of 2,3,7,8-TCDD (0, 0.25, 0.5, 1, 2.5, 5, 10, 25 nM) for 24 or 48 h. MTT and Comet assays were performed to determine cytotoxicities and genotoxicities to select exposure concentrations for the proteomic analysis of proteins secreted by 2,3,7,8-TCDD-treated cells. In the proteomic analysis, dose- and time-dependent toxicological biomarkers were evaluated using two pI ranges (4-7 and 6-9) using a large gel 2-DE system. Fifteen secreted proteins were identified by a nano-LC-ESI-MS/MS and nano-ESI on a Q-TOF2 MS and the identities of eight secreted proteins including glyoxalase 1 (GLO 1), homogentisate dioxygenase (HGD), peroxiredoxin 1 (PRX 1), proteasome subunit beta type (PSMB) 5 and 6, UDP-glucose 6-dehydrogenase (UDP-GlcDH), hydroxyacyl-coenzyme A dehydrogenase (HADH) and serotransferrin (STF) were confirmed by western blotting. Of these, PSMB 5 and PRX 1 were also found in the plasma of rats exposed to 2,3,7,8-TCDD, whereas GLO 1, HGD, PSMB 6 and PRX 1 were found in the plasma of incineration workers exposed to dioxins. Copyright © 2016. Published by Elsevier B.V.

  12. Clinical and laboratory evaluation of 101 patients with intrahepatic neonatal cholestasis Avaliação clínica e laboratorial de 101 pacientes com colestase neonatal intra-hepática

    Directory of Open Access Journals (Sweden)

    Maria Angela Bellomo-Brandão

    2008-06-01

    Full Text Available BACKGROUND: Intrahepatic neonatal cholestasis can be the initial manifestation of a very heterogeneous group of illnesses of different etiologies. AIM: To evaluate and compare clinical and laboratory data among intrahepatic neonatal cholestasis groups of infectious, genetic-endocrine-metabolic and idiopathic etiologies. METHODS: The study evaluated retrospectively clinical and laboratory data of 101 infants, from March 1982 to December 2005, 84 from the State University of Campinas Teaching Hospital, Campinas, SP, Brazil, and 17 from the Child’s Institute of the University of São Paulo, SP, Brazil. The inclusion criteria consisted of: jaundice beginning at up to 3 months of age and hepatic biopsy during the 1st year of life. It had been evaluated: clinical findings (gender, age, birth weight, weight during the first medical visit, stature at birth, jaundice, acholia/hipocholia, choluria, hepatomegaly and splenomegaly and laboratorial (ALT, AST, FA, GGT, INR. RESULTS: According to diagnosis, patients were classified into three groups: group 1 (infectious n = 24, group 2 (genetic-endocrine-metabolic n = 21 and group 3 (idiopathic n = 56. There were no significant differences in relation to the variables: age, gender, stature at birth, jaundice, acholia/hipocholia, choluria, hepatomegaly, splenomegaly, AST, ALT, ALP, GGT, DB and albumin. Significant differences were observed in relation to the following variables: birth weight and weight during the first medical visit. Birth weight of group 1 was lower in relation group 2 and 3. Weight during the first medical visit followed the same pattern. There was a statistically significant difference in relation to the INR, as the patients of the group 2 presented higher values in relation to groups 2 and 3, despite the median was still pointing out normal values. CONCLUSIONS: There were no significant differences in relation to age, gender, stature at birth, jaundice, acholia/hipocholia, choluria

  13. Nonalcoholic fatty liver disease: histopathological evaluation and interobserver agreement Doença hepática gordurosa não-alcoólica: avaliação histopatológica e concordância interobservador

    Directory of Open Access Journals (Sweden)

    Cynthia Koeppel Berenstein

    2008-02-01

    Full Text Available BACKGROUND: Nonalcoholic fatty liver disease comprises a spectrum of lesions ranging from steatosis to cirrhosis, with nonalcoholic steatohepatitis being the progressive form of the disease. Alcohol intake, viral hepatitis and other liver diseases must be excluded. Liver biopsy is the gold standard for diagnosis of the disease and is the only method able to differentiate nonalcoholic steatohepatitis from simple steatosis, to grade inflammation and to stage fibrosis. AIMS: To analyze the histopathological findings and evaluate interobserver agreement in biopsies previously diagnosed as steatosis or steatohepatitis. METHODS: Seventy needle biopsies were analyzed according to Brunt et al.(4, with modifications in the grading and staging components. Clinical data of patients were collected. Interobserver agreement was calculated based on histopathological findings. RESULTS: Mild nonalcoholic steatohepatitis (grade 1 was the most common form. If fibrosis was detected, stage 1 was the most frequent. Interobserver agreement was very good for macrovesicular steatosis (K W = 0,82 and good for lobular inflammation (K W = 0,68 and fibrosis (K W = 0,73. CONCLUSIONS: The classification of Brunt et al., with modifications, can be applied to diagnosis not only of nonalcoholic steatohepatitis but also of nonalcoholic fatty liver disease, representing a reliable method for use in the daily practice of pathologists.INTRODUÇÃO: A doença hepática gordurosa não-alcoólica compreende um espectro de lesões que variam da esteatose à cirrose, sendo a esteatoepatite não-alcoólica a forma progressiva da doença. Uso de álcool, hepatites virais e outras doenças hepáticas devem ser excluídos. A biópsia hepática é o padrão-ouro para o diagnóstico da doença, sendo o único método capaz de diferenciar a esteatoepatite da esteatose, graduar a inflamação e estadiar a fibrose. OBJETIVOS: Analisar os achados histopatológicos e avaliar a concord

  14. How Does Procedural Fairness Affect Performance Evaluation System Satisfaction? (Evidence from a UK Police Force

    Directory of Open Access Journals (Sweden)

    Sholihin Mahfud

    2013-12-01

    Full Text Available This paper investigates whether, and if so, how procedural fairness affects performance evaluation system satisfaction in a UK Police Force. Employing a survey method with samples of detective inspectors and detective chief inspectors with significant managerial responsibilities in a UK Police Force, this study finds that procedural fairness affects performance evaluation system satisfaction. Further analysis reveals that the effect of procedural fairness on performance evaluation system satisfaction is mediated by trust. This study provides empirical evidence on how procedural fairness affects performance evaluation system satisfaction. The results of this study may benefit the designer of performance evaluation systems in police organizations.

  15. Silicon Sensors for HEP Experiments

    CERN Document Server

    Dierlamm, Alexander Hermann

    2017-01-01

    With increasing luminosity of accelerators for experiments in High Energy Physics the demands on the detectors increase as well. Especially tracking and vertexing detectors made of silicon sensors close to the interaction point need to be equipped with more radiation hard devices. This article introduces the different types of silicon sensors, describes measures to increase radiation hardness and provides an overview of present upgrade choices of HEP experiments.

  16. Evaluation of Revised Computer-Based Procedure System Prototype

    Energy Technology Data Exchange (ETDEWEB)

    Katya Le Blanc; Johanna Oxstrand; Cheradan Fikstad

    2013-01-01

    The nuclear power industry is very procedure driven, i.e. almost all activities that take place at a nuclear power plant are conducted by following procedures. The paper-based procedures (PBPs) currently used by the industry do a good job at keeping the industry safe. However, these procedures are most often paired with methods and tools put in place to anticipate, prevent, and catch errors related to hands-on work. These tools are commonly called human performance tools. The drawback with the current implementation of these tools is that the task of performing one procedure becomes time and labor intensive. For example, concurrent and independent verification of procedure steps are required at times, which essentially means that at least two people have to be actively involved in the task. Even though the current use of PBPs and human performance tools are keeping the industry safe, there is room for improvement. The industry could potentially increase their efficiency and safety by replacing their existing PBPs with CBPs. If implemented correctly, the CBP system could reduce the time and focus spent on using the human performance tools. Some of the tools can be completely incorporated in the CBP system in a manner that the performer does not think about the fact that these tools are being used. Examples of these tools are procedure use and adherence, placekeeping, and peer checks. Other tools can be partly integrated in a fashion that reduce the time and labor they require, such as concurrent and independent verification. The incorporation of advanced technology, such as CBP systems, may help to manage the effects of aging systems, structures, and components. The introduction of advanced technology may also make the existing LWR fleet more attractive to the future workforce, which will be of importance when the future workforce will chose between existing fleet and the newly built nuclear power plants.

  17. Development of Procedures to Evaluate Hot Section Deterioration for Marine Gas Turbines.

    Science.gov (United States)

    each procedure were discussed. It was demonstrated that reliable comparisons between hot stage component performance of different engiens can best be obtained by utilization of multiple evaluation methods.

  18. evaluation of approximate design procedures for biaxially loaded ...

    African Journals Online (AJOL)

    testing the validity of the /3-n charts and presenting tbe approximate procedure for the design of biaxially loaded columns according to the ACI. For rectangular cross-sections with equal relative cover ratios and doubly symmetric reinforcement pattern, the relative uniaxial moment capacities are equal. Thus letting muy = mu, ...

  19. Evaluation of fire hazard inspection procedures in Butte County, California

    Science.gov (United States)

    William S. Folkman

    1967-01-01

    To assess effectiveness of fire hazard inspection procedures in securing compliance with fire safety requirements, effects of different types and combinations of contacts and timing were determined, and the production capacity of inspectors measured. It was demonstrated that fire law inspection is as much fire prevention education and engineering as it is law...

  20. Evaluation of selection procedures of an international school | O ...

    African Journals Online (AJOL)

    Results indicated that the admission procedures compared favourably with those of the other international schools, and that the entrance tests had a significant predictive value. The Culture-Fair Intelligence Test (Scale 2 Form A) appeared to have more predictive value than the MAT-SF for academic achievement. Academic ...

  1. MAPS evaluation report and procedures governing interviews and performance appraisals

    CERN Multimedia

    HR Department

    2006-01-01

    Following various improvements to the MAPS report and to the procedures governing interviews and performance appraisals (announced in the CERN Bulletin 48-49/2005), a third information session has been organized for all staff members on Tuesday, 31 January at 10 a.m.: AB Auditorium P (864-1-D02), Human Resources Department Tel. 73566

  2. A multicenter evaluation of a new therapeutic plasma exchange procedure.

    Science.gov (United States)

    Winters, Jeffrey L; Burgstaler, Edwin A; Gottschall, Jerome L; Balogun, Rasheed A; Houghton, Jaime R; Lee, Wanda J; Snyder, Edward L

    2013-12-01

    The AMICUS (Fenwal, Inc.) was cleared in the United States for platelet (PLT) and plasma collection in 1996 with subsequent clearances for the collection of other blood products. Although not previously used for therapeutic plasma exchange (TPE), new disposables, software, and hardware were developed to enable TPE on the AMICUS. A multicenter, randomized, nonblinded, crossover paired treatment protocol was performed. Thirty patients with orders for at least two TPE procedures were randomly assigned to the AMICUS or the COBE Spectra (TerumoBCT) for the first treatment. Each patient was crossed over to the other device using the same procedure settings from the first procedure. The primary objective compared efficiency of plasma removal (EPR) with secondary objectives of comparing PLT and hemoglobin (Hb) waste plasma content, coagulation factor and complement activation, fluid balance tracking accuracy, procedure length, and adverse events. The EPR for the AMICUS (81.9 ± 7.62%) was superior to that of the COBE Spectra (75.2 ± 6.29%; p = 0.00001). The AMICUS also demonstrated statistically higher fluid balance accuracy (99.84%) compared to that of the COBE Spectra (98.83%; p < 0.0001) and a statistically shorter procedure time (103.9 ± 30.8 vs. 110.5 ± 27.1 min, p < 0.001). No significant differences with regard to PLT and Hb content in the waste plasma, change in patient PLT count, or changes in markers of coagulation and complement cascade activation were seen. Frequency and severity of adverse reactions were similar. The AMICUS separator can effectively perform TPE. The AMICUS demonstrated superior plasma removal efficiency compared to the COBE Spectra with no evidence of significant differences in PLT removal, hemolysis, and coagulation or complement activation. © 2013 American Association of Blood Banks.

  3. MAPS evaluation report and procedures governing interviews and performance appraisals

    CERN Multimedia

    HR Department

    2006-01-01

    Following various improvements made to the MAPS report and to the procedures governing interviews and performance appraisals (announced in the CERN Weekly Bulletin 48-49/2005), three information sessions have been organized for all staff members: 24 January 10:00: AB Auditorium P (864-1-D02), 26 January 14:00: Main Amphitheatre, 31 January 10:00: AB Auditorium P (864-1-D02). Human Resources Department Tel. 73566

  4. Determining the quality of competences assessment programs: A self-evaluation procedure

    NARCIS (Netherlands)

    Baartman, Liesbeth; Prins, Frans; Kirschner, Paul A.; Van der Vleuten, Cees

    2009-01-01

    Baartman, L. K. J., Prins, F. J., Kirschner, P. A., & Van der Vleuten, C. P. M. (2007). Determining the quality of Competence Assessment Programs: A self-evaluation procedure. Studies in Educational Evaluation, 33, 258-281.

  5. Benchmarking Cloud Resources for HEP

    Science.gov (United States)

    Alef, M.; Cordeiro, C.; De Salvo, A.; Di Girolamo, A.; Field, L.; Giordano, D.; Guerri, M.; Schiavi, F. C.; Wiebalck, A.

    2017-10-01

    In a commercial cloud environment, exhaustive resource profiling is beneficial to cope with the intrinsic variability of the virtualised environment, allowing to promptly identify performance degradation. In the context of its commercial cloud initiatives, CERN has acquired extensive experience in benchmarking commercial cloud resources. Ultimately, this activity provides information on the actual delivered performance of invoiced resources. In this report we discuss the experience acquired and the results collected using several fast benchmark applications adopted by the HEP community. These benchmarks span from open-source benchmarks to specific user applications and synthetic benchmarks. The workflow put in place to collect and analyse performance metrics is also described.

  6. DOT-7A Type A packaging test and evaluation procedure

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, D.L., Westinghouse Hanford

    1996-06-13

    The purpose of this document is to provide guidance for qualifying a DOT-7A Type A packaging for use. WHC qualifies DOT-7A packaging for two purposes. The first is to provide packages for use by WHC (manufacturer-qualified). The second is to provide a contracted service in support of DOE/EM-76 (DOE-qualified). This document includes descriptions of the performance tests, the personnel involved and their qualifications, appropriate safety and quality assurance considerations, and the procedures to be followed when WHC performs the tests (either as the manufacturer, or on behalf of the DOE`s certification program).

  7. Evaluation Manual for CIP Courses: Objectives and Implementation Procedures.

    Science.gov (United States)

    Siri, Carmen

    This manual has been designed to guide courses on potato production sponsored by the International Potato Center (CIP) in Lima (Peru). It describes the CIP Course Evaluation System that is presently used and provides guidelines on how to use feedback more effectively for improving training. CIP evaluations are largely formative. The CIP focuses on…

  8. A Procedural Electroencephalogram Simulator for Evaluation of Anesthesia Monitors.

    Science.gov (United States)

    Petersen, Christian Leth; Görges, Matthias; Massey, Roslyn; Dumont, Guy Albert; Ansermino, J Mark

    2016-11-01

    Recent research and advances in the automation of anesthesia are driving the need to better understand electroencephalogram (EEG)-based anesthesia end points and to test the performance of anesthesia monitors. This effort is currently limited by the need to collect raw EEG data directly from patients. A procedural method to synthesize EEG signals was implemented in a mobile software application. The application is capable of sending the simulated signal to an anesthesia depth of hypnosis monitor. Systematic sweeps of the simulator generate functional monitor response profiles reminiscent of how network analyzers are used to test electronic components. Three commercial anesthesia monitors (Entropy, NeuroSENSE, and BIS) were compared with this new technology, and significant response and feature variations between the monitor models were observed; this includes reproducible, nonmonotonic apparent multistate behavior and significant hysteresis at light levels of anesthesia. Anesthesia monitor response to a procedural simulator can reveal significant differences in internal signal processing algorithms. The ability to synthesize EEG signals at different anesthetic depths potentially provides a new method for systematically testing EEG-based monitors and automated anesthesia systems with all sensor hardware fully operational before human trials.

  9. Evaluating restorative materials and procedures in dental practice.

    Science.gov (United States)

    Burke, F J T

    2005-12-01

    A wide variety of research methods are appropriate to general dental practice, including clinical trials of materials, assessment of materials and techniques, treatment trends, and assessment of behavior and attitudes, of dentists as well as patients. This paper will describe the use of practice-based networks to evaluate the effectiveness of materials and techniques in dental practice. Several practice-based research groups are presently in operation in the UK and the USA, generally carrying out evaluations of the handling of materials, but with increasing emphasis on the clinical evaluation of restorations. Use of the Dental Practice Board (of England and Wales) database has proved to be a fruitful source of data on the long-term outcome of restorations. Dental practice can provide the large pool of patients available for research. To utilize this pool of patients, dental practitioners and their support staff require training in collecting data.

  10. Evaluation of Surface Infiltration Testing Procedures in Permeable Pavement Systems

    Science.gov (United States)

    The ASTM method (ASTM C1701) for measuring infiltration rate of in-place pervious concrete provides limited guidance on how to select testing locations, so research is needed to evaluate how testing sites should be selected and how results should be interpreted to assess surface ...

  11. Evaluation of selection procedures of an international school

    African Journals Online (AJOL)

    Erna Kinsey

    Department of Psychology, University of Stellenbosch, Private Bag X1, Matieland, 7602 South Africa ... combination of the Culture-Fair Intelligence Test (Scale 2 Form A), the MAT-SF, the English proficiency test and the form (level of progress ... chometric testing, evaluation and selection (Samuda, Feuerstein, Kauf-.

  12. Innovative procedures to evaluate corn silage for milk yield

    Directory of Open Access Journals (Sweden)

    C. Zanfi

    2010-01-01

    Full Text Available Corn silage is the main ingredient of diets for dairy cattle (around 40% of diet DM in Italy and, therefore, an accurate estimation of its nutritive value is essential to describe the whole diet. Given the low, and fairly stable protein, lipid and ash contents in corn silage (e.g. a total of around 15% DM, the critical point to evaluate its energy value is the amount and availability of the two main carbohydrate fractions (NDF and non fiber carbohydrates, NFC.

  13. The osteoprotective effect of Herba epimedii (HEP extract in vivo and in vitro

    Directory of Open Access Journals (Sweden)

    Fang Xie

    2005-01-01

    Full Text Available Herba epimedii (HEP is one of the most frequently used herbs prescribed for treatment of osteoporosis in China. In the present study, the in vivo effects of HEP extract on bone metabolism were evaluated using 4-month-old ovariectomized (OVX or sham-operated (Sham female Sprague-Dawley rats orally administered with HEP extract (110 mg kg−1d−1, 17ß-estrogen (2 mg kg−1d−1 or its vehicle for 3 months. HEP extract significantly decreased urinary calcium excretion, suppressed serum alkaline phosphatase (ALP activity and urinary deoxypyridinoline levels in OVX rats (P < 0.05 versus vehicle-treated OVX rats. Histomorphometric analysis indicated that HEP extract could prevent OVX-induced bone loss by increasing tibial trabecular bone area and decreasing trabecular separation in OVX rats (P < 0.05 versus vehicle-treated OVX group. The in vitro effects of HEP extract were also studied using rat osteoblast-like UMR 106 cells. HEP extract significantly stimulated cell proliferation in a dose-dependent manner (P < 0.01 versus vehicle-treated and increased ALP activity at 200 μgml−1 (P < 0.01 versus vehicle-treated in UMR 106 cells. It modulated osteoclastogenesis by increasing osteoprotegrin (OPG mRNA and decreasing receptor activator of NF-κB ligand (RANKL mRNA expression, resulting in a dose-dependent increase in OPG/RANKL mRNA ratio (P < 0.01 versus vehicle-treated. Taken together, HEP treatment can effectively suppress the OVX-induced increase in bone turnover possibly by both an increase in osteoblastic activities and a decrease in osteoclastogenesis. The present study provides the evidence that HEP can be considered as a complementary and alternative medicine for treatment of post-menopausal osteoporosis.

  14. Practical evaluation procedure to assess and remediate speech perception skills.

    Science.gov (United States)

    Vergara, K C; Miskiel, L W; Oller, D K; Eilers, R E

    1997-01-01

    The University of Miami/Dade County Public Schools Model Program for the Deaf and Hard of Hearing is a research and training effort dedicated to the utilization of sensory aids including hearing aids, tactual vocoders, and cochlear implants. The program's teachers and clinicians follow the Miami Cochlear Implant, Auditory, and Tactile Skills (CHATS) Curriculum for the development of individualized speech perception and production goals. A series of peech perception tests has been used for the past five years to evaluate the children's progress. The test battery, administered at six month intervals, is extensive and impractical for school clinicians and teachers to administer to their students. To assist teachers and clinicians in the process of selecting appropriate goals and objectives for sensory aid training, a speech perception test has been developed to accompany the curriculum. This paper includes a discussion of the test design as it correlates with the curriculum.

  15. Evaluation of APREQCFR Coordination Procedures for Charlotte Douglas International Airport

    Science.gov (United States)

    Stevens, Lindsay K. S.; Parke, Bonny K.; Chevalley, Eric; Lee, Hanbong; Martin, Lynne H.; Jobe, Kimberly K.; Verma, Savita A.; Dulchinos, Victoria Lee

    2017-01-01

    NASA has been collaborating with the Federal Aviation Administration (FAA) and aviation industry partners to develop and demonstrate new concepts and technologies for the Integrated Arrival, Departure, and Surface (IADS) traffic management capabilities under the Airspace Technology Demonstration 2 (ATD-2) project. One of the goal of The IADS capabilities in the ATD-2 project is to increase predictability and increase throughput via improving TMI compliance. The IADS capabilities that will impact TMI compliance are built upon previous NASA research, the Precision Departure Release Capability (PDRC). The proposed paper will evaluate the APREQCFR process between ATC Tower and Center and information sharing between ATC Tower and the airline Ramp tower. Subjective measures collected from the HITL surveys (e.g., workload, situational awareness, acceptability, usability) and performance metrics such as TMI, TMAT, and pushback advisory compliance from APREQCFR flights and will be reported.

  16. Evaluation of three electrodeposition procedures for uranium, plutonium and americium.

    Science.gov (United States)

    Oh, Jung-Suk; Warwick, Phillip E; Croudace, Ian W; Lee, Sang-Han

    2014-05-01

    While both mass spectrometry and alpha-particle spectrometry have been widely used for measuring alpha activities, the former is preferred since many laboratories are not equipped with ICP-MS and MC-ICP-MS systems. In this study, three electrodeposition techniques using ammonium chloride, ammonium oxalate and ammonium sulphate as electrolyte solutions were applied and evaluated for the preparation of uranium, plutonium and americium sources for alpha-particle spectrometry. Changes in pH and temperature throughout the electrodeposition process were measured every 15 min, together with percentage deposition every 30 min. The percentage deposition in each method was checked at 300, 400 and 500 mA, and the optimised time and current were determined. © 2013 Published by Elsevier Ltd.

  17. THE PROCEDURE BY EVALUATION OF DIVERS FOR THE DEEP DIVING

    Directory of Open Access Journals (Sweden)

    Mihai DIACONU

    2014-05-01

    Full Text Available When a diver is selected to perform a deep diving course, he has to pass a medical test and isn’t affected by the HPNS (High Pressure Nervous Syndrome. When a diver is selected to perform a deep diving course, he has to pass an evaluation stage in order to prove his ability to tolerate the compression speed accompanied by the helium - oxygen breathing mixture without encountering the HPNS effects. The deep diving test consists in postural tremor and the EEG registration, in both conditions: at the atmospheric pressure and at 180 m deep too. Then the results obtained at the surface percentage limits are fixed for the maximum deflection agreed between the values registration made under pressure and the atmospheric pressure (surface values.

  18. Kosher slaughter paradigms: Evaluation of slaughterhouse inspection procedures.

    Science.gov (United States)

    Bozzo, Giancarlo; Di Pinto, Angela; Bonerba, Elisabetta; Ceci, Edmondo; Mottola, Anna; Roma, Rocco; Capozza, Paolo; Samoilis, Giorgio; Tantillo, Giuseppina; Celano, Gaetano Vitale

    2017-06-01

    Slaughter by Jewish religious rite is the killing of an animal by cutting the trachea and oesophagus and major blood vessels using a very sharp blade. This operation is subject to strict rules laid down by religious authorities that characterize its sacredness. The aim of the study was to evaluate the specific criteria inherent in the Jewish religious rite, by analysing reject rates during the different phases. In this study, 52.4% of the carcasses failed to quality as Kosher, with 22.9% being rejected due to pulmonary lesions and only 3% for miscuts. The study also revealed legal vacuums in the field of labelling rules. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Development of Evaluation Procedure for Effective Implementation of Cdio

    DEFF Research Database (Denmark)

    Christensen, Jørgen Erik; Rode, Carsten; Borchersen, Egil

    2009-01-01

    of Denmark (DTU), the CDIO approach was started in the autumn of 2008 in the process of reforming the engineering education in order to educate the students to become more effective engineers. One of the goals is to effectively implement CDIO practices and reduce time for implementation. One of the ways...... the presentation day) compared to only 45% at the electronic inquiry at the CampusNet – giving the paper inquiry form a far more representative value. Altogether, this material has given the CDIO staff very good material for the evaluation of the CDIO Design Build course and input for improvement and effective......One of the challenges in modern engineering education is the demand for teaching of high quality where the subject is presented in an interesting and engaging way. By integrating and involving the students in the teaching process, the learning can be increased. At the Technical University...

  20. Statistical procedures for evaluating daily and monthly hydrologic model predictions

    Science.gov (United States)

    Coffey, M.E.; Workman, S.R.; Taraba, J.L.; Fogle, A.W.

    2004-01-01

    The overall study objective was to evaluate the applicability of different qualitative and quantitative methods for comparing daily and monthly SWAT computer model hydrologic streamflow predictions to observed data, and to recommend statistical methods for use in future model evaluations. Statistical methods were tested using daily streamflows and monthly equivalent runoff depths. The statistical techniques included linear regression, Nash-Sutcliffe efficiency, nonparametric tests, t-test, objective functions, autocorrelation, and cross-correlation. None of the methods specifically applied to the non-normal distribution and dependence between data points for the daily predicted and observed data. Of the tested methods, median objective functions, sign test, autocorrelation, and cross-correlation were most applicable for the daily data. The robust coefficient of determination (CD*) and robust modeling efficiency (EF*) objective functions were the preferred methods for daily model results due to the ease of comparing these values with a fixed ideal reference value of one. Predicted and observed monthly totals were more normally distributed, and there was less dependence between individual monthly totals than was observed for the corresponding predicted and observed daily values. More statistical methods were available for comparing SWAT model-predicted and observed monthly totals. The 1995 monthly SWAT model predictions and observed data had a regression Rr2 of 0.70, a Nash-Sutcliffe efficiency of 0.41, and the t-test failed to reject the equal data means hypothesis. The Nash-Sutcliffe coefficient and the R r2 coefficient were the preferred methods for monthly results due to the ability to compare these coefficients to a set ideal value of one.

  1. [Penile protheses multicentre practice evaluation, results after 282 procedures].

    Science.gov (United States)

    Menard, Johann; Tremeaux, Jack-Charles; Faix, Antoine; Staerman, Frédéric

    2007-04-01

    Multicentre practice evaluation in the field of penile prostheses based on the Club des Implanteurs de Prosthèses Péniennes (CIPP) database. 282 penile prostheses, including 276 inflatable prostheses (97.8%), were implanted in 254 patients between 1996 and 2005 in three centres (1 public and 2 private). Preoperative data (patient's age, aetiology and duration of erectile dysfunction, preliminary treatments) and intraoperative data (type of implant used, surgical approach) were recorded. Postoperative complications were studied and postoperative erectile function was evaluated by a self-administered questionnaire (IIEF). The mean follow-up was 27.7 months. Penile prostheses were implanted after an average of 39.2 months of erectile dysfunction in patients with a mean age of 58.6 years. The main aetiologies were arterial disease (35.3%), diabetes (22.8%) and radical prostatectomy (16.5%). The postoperative sepsis rate was 2.2% and the mechanical dysfunction rate was 7.5% at the beginning of the operators' experience. The rate of other complications requiring repeat surgery (erosion, migration, self-inflation) was 1.8% at the beginning of the operators' experience. The septic risk was increased (7.6%) in the presence of diabetes (p = 0.01). The postoperative overall satisfaction was 86.7% and the IIEF score increased from 20.5 +/- 11.8 preoperatively to 68.9 +/- 5.4 postoperatively. Penile prostheses achieve a high postoperative satisfaction score with a low complication rate. However, they are only indicated after failure of less invasive treatments and must be implanted by experienced operators. These results are comparable to those of large international single-centre series.

  2. Analysis Traceability and Provenance for HEP

    Science.gov (United States)

    Shamdasani, Jetendr; McClatchey, Richard; Branson, Andrew; Kovács, Zsolt

    2015-12-01

    This paper presents the use of the CRISTAL software in the N4U project. CRISTAL was used to create a set of provenance aware analysis tools for the Neuroscience domain. This paper advocates that the approach taken in N4U to build the analysis suite is sufficiently generic to be able to be applied to the HEP domain. A mapping to the PROV model for provenance interoperability is also presented and how this can be applied to the HEP domain for the interoperability of HEP analyses.

  3. Estilos de manejo familiar: uma possibilidade de avaliação no transplante hepático pediátrico Estilos de manejo familiar: una posibilidad de evaluación en el transplante hepático pediátrico Family management styles: a possibility of evaluation in pediatric liver transplant

    Directory of Open Access Journals (Sweden)

    Ana Márcia Chiaradia Mendes-Castillo

    2012-01-01

    Full Text Available OBJETIVO: Identificar os estilos de manejo familiar durante a experiência do transplante hepático da criança, de acordo com o Family Management Style Framework. MÉTODOS: Estudo descritivo, com abordagem qualitativa, realizado mediante uma análise secundária de nove entrevistas semiestruturadas, previamente coletadas com oito famílias que tiveram uma criança que atravessava a experiência de transplante hepático. RESULTADOS: Pela análise, foi possível identificar cinco estilos de manejo: família ajustada, família em adaptação, família lutando, família em conflito e família em espera. CONCLUSÃO: O modelo mostrou-se útil na avaliação de famílias no contexto do transplante pediátrico e seu uso é encorajado neste e em outros cenários de doença crônica.OBJETIVO: Identificar los estilos de manejo familiar durante la experiencia del transplante hepático del niño, de acuerdo con el Family Management Style Framework. MÉTODOS: Estudio descriptivo, con abordaje cualitativo, realizado mediante un análisis secundario de nueve entrevistas semiestructuradas, previamente recolectadas con ocho familias que tuvieron una niño que atravesaba la experiencia del transplante hepático. RESULTADOS: Por el análisis, fue posible identificar cinco estilos de manejo: familia ajustada, familia en adaptación, familia luchando, familia en conflicto y familia en espera. CONCLUSIÓN: El modelo se mostró útil en la evaluación de familias en el contexto del transplante pediátrico y su uso es incentivado en éste y en otros escenarios de enfermedad crónica.OBJECTIVE: To identify the styles of family management during the experience of liver transplantation of a child, according to the Family Management Style Framework. METHODS: A descriptive study with a qualitative approach, conducted by means of a secondary analysis of nine semi-structured interviews previously collected with eight families who had a child who experienced a liver

  4. Estudo comparativo dos procedimentos percutâneos orientados por métodos de imagem no tratamento das coleções hepáticas A comparative study of image-guided percutaneous procedures for the treatment of liver abscesses

    Directory of Open Access Journals (Sweden)

    Giselle Guedes Netto de Mello

    2001-04-01

    Full Text Available OBJETIVO: Verificar e comparar a eficácia da punção aspirativa e da drenagem percutânea, orientadas por métodos de imagem, no tratamento das coleções hepáticas. MATERIAIS E MÉTODOS: Foram encaminhados, ao nosso setor, 52 pacientes para tratamento percutâneo de coleções abdominais. Destes, 17 apresentavam coleções hepáticas, sendo 13/17 não-complexas e 4/17, complexas (multiloculadas, múltiplas ou associadas a fístulas. Foram submetidas a punção aspirativa 7/17 coleções (41,2% e a drenagem percutânea, 10/17 (58,8%. Considerou-se como sucesso do método a resolução completa da coleção, com melhora clínica e laboratorial do paciente. RESULTADOS: O sucesso do procedimento ocorreu em 82,4% dos casos. Nas coleções submetidas exclusivamente a punção aspirativa, obteve-se sucesso em 57,1%, enquanto nas submetidas a drenagem percutânea o sucesso foi de 100%. O índice de sucesso no grupo submetido a punção aspirativa foi de 75% nas coleções menores que 100 ml, e de 33,3% naquelas entre 100 e 250 ml. Houve sucesso com a punção aspirativa em 75% das coleções não-complexas e em 25% das complexas. CONCLUSÃO: A drenagem percutânea é mais eficaz que a punção aspirativa no tratamento das coleções hepáticas. A punção aspirativa talvez possa ser empregada como alternativa válida nas coleções de menor volume e não-complexas.OBJECTIVE: To determine and compare the efficacy of percutaneous needle aspiration and percutaneous catheter drainage, both guided by imaging methods, for the treatment of liver abscesses. MATERIALS AND METHODS: From 52 patients referred to our service for percutaneous treatment of abdominal abscesses, 17 presented liver abscesses, 13 of which were considered noncomplex and four were considered complex (multiloculated, multiple or associated to fistulas. Percutaneous needle aspiration was performed in 7/17 patients and 10/17 patients were submitted to percutaneous catheter drainage. The

  5. A modern approach to HEP visualization - ATLASrift

    CERN Document Server

    Vukotic, Ilija; The ATLAS collaboration

    2017-01-01

    At the times when HEP computing needs were mainly fulfilled by mainframes, graphics solutions for event and detector visualizations were necessarily hardware as well as experiment specific and impossible to use anywhere outside of HEP community. A big move to commodity computing did not precipitate a corresponding move of graphics solutions to industry standard hardware and software. In this paper, we list functionalities expected from contemporary tools and describe their implementation by a specific application: ATLASrift. We start with a basic premise that HEP visualization tools should be open in practice and not only in intentions. This means that a user should not be limited to specific and little used platforms, HEP-only software packages, or experiment-specific libraries. Equally important is that no special knowledge or special access rights are needed. Using industry standard frameworks brings not only sustainability, but also good support, a lot of community contributed tools, and a possibility of ...

  6. ASCR/HEP Exascale Requirements Review Report

    Energy Technology Data Exchange (ETDEWEB)

    Habib, Salman; et al.

    2016-03-30

    This draft report summarizes and details the findings, results, and recommendations derived from the ASCR/HEP Exascale Requirements Review meeting held in June, 2015. The main conclusions are as follows. 1) Larger, more capable computing and data facilities are needed to support HEP science goals in all three frontiers: Energy, Intensity, and Cosmic. The expected scale of the demand at the 2025 timescale is at least two orders of magnitude -- and in some cases greater -- than that available currently. 2) The growth rate of data produced by simulations is overwhelming the current ability, of both facilities and researchers, to store and analyze it. Additional resources and new techniques for data analysis are urgently needed. 3) Data rates and volumes from HEP experimental facilities are also straining the ability to store and analyze large and complex data volumes. Appropriately configured leadership-class facilities can play a transformational role in enabling scientific discovery from these datasets. 4) A close integration of HPC simulation and data analysis will aid greatly in interpreting results from HEP experiments. Such an integration will minimize data movement and facilitate interdependent workflows. 5) Long-range planning between HEP and ASCR will be required to meet HEP's research needs. To best use ASCR HPC resources the experimental HEP program needs a) an established long-term plan for access to ASCR computational and data resources, b) an ability to map workflows onto HPC resources, c) the ability for ASCR facilities to accommodate workflows run by collaborations that can have thousands of individual members, d) to transition codes to the next-generation HPC platforms that will be available at ASCR facilities, e) to build up and train a workforce capable of developing and using simulations and analysis to support HEP scientific research on next-generation systems.

  7. ASCR/HEP Exascale Requirements Review Report

    Energy Technology Data Exchange (ETDEWEB)

    Habib, Salman [Argonne National Lab. (ANL), Argonne, IL (United States); Roser, Robert [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Gerber, Richard [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Antypas, Katie [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Riley, Katherine [Argonne National Lab. (ANL), Argonne, IL (United States); Williams, Tim [Argonne National Lab. (ANL), Argonne, IL (United States); Wells, Jack [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Straatsma, Tjerk [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Almgren, A. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Amundson, J. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Bailey, S. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Bard, D. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Bloom, K. [Univ. of Nebraska, Lincoln, NE (United States); Bockelman, B. [Univ. of Nebraska, Lincoln, NE (United States); Borgland, A. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Borrill, J. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Boughezal, R. [Argonne National Lab. (ANL), Argonne, IL (United States); Brower, R. [Boston Univ., MA (United States); Cowan, B. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Finkel, H. [Argonne National Lab. (ANL), Argonne, IL (United States); Frontiere, N. [Argonne National Lab. (ANL), Argonne, IL (United States); Fuess, S. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Ge, L. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Gnedin, N. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Gottlieb, S. [Indiana Univ., Bloomington, IN (United States); Gutsche, O. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Han, T. [Univ. of Pittsburgh, PA (United States); Heitmann, K. [Argonne National Lab. (ANL), Argonne, IL (United States); Hoeche, S. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Ko, K. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Kononenko, O. [SLAC National Accelerator Lab., Menlo Park, CA (United States); LeCompte, T. [Argonne National Lab. (ANL), Argonne, IL (United States); Li, Z. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Lukic, Z. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Mori, W. [Univ. of California, Los Angeles, CA (United States); Nugent, P. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Ng, C. -K. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Oleynik, G. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); O' Shea, B. [Michigan State Univ., East Lansing, MI (United States); Padmanabhan, N. [Yale Univ., New Haven, CT (United States); Petravick, D. [Univ. of Illinois, Urbana-Champaign, IL (United States). National Center for Supercomputing Applications (NCSA); Petriello, F. J. [Argonne National Lab. (ANL), Argonne, IL (United States); Power, J. [Argonne National Lab. (ANL), Argonne, IL (United States); Qiang, J. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Reina, L. [Florida State Univ., Tallahassee, FL (United States); Rizzo, T. J. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Ryne, R. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Schram, M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Spentzouris, P. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Toussaint, D. [Univ. of Arizona, Tucson, AZ (United States); Vay, J. -L. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Viren, B. [Brookhaven National Lab. (BNL), Upton, NY (United States); Wurthwein, F. [Univ. of California, San Diego, CA (United States); Xiao, L. [SLAC National Accelerator Lab., Menlo Park, CA (United States)

    2017-02-06

    This draft report summarizes and details the findings, results, and recommendations derived from the ASCR/HEP Exascale Requirements Review meeting held in June, 2015. The main conclusions are as follows. 1) Larger, more capable computing and data facilities are needed to support HEP science goals in all three frontiers: Energy, Intensity, and Cosmic. The expected scale of the demand at the 2025 timescale is at least two orders of magnitude -- and in some cases greater -- than that available currently. 2) The growth rate of data produced by simulations is overwhelming the current ability, of both facilities and researchers, to store and analyze it. Additional resources and new techniques for data analysis are urgently needed. 3) Data rates and volumes from HEP experimental facilities are also straining the ability to store and analyze large and complex data volumes. Appropriately configured leadership-class facilities can play a transformational role in enabling scientific discovery from these datasets. 4) A close integration of HPC simulation and data analysis will aid greatly in interpreting results from HEP experiments. Such an integration will minimize data movement and facilitate interdependent workflows. 5) Long-range planning between HEP and ASCR will be required to meet HEP's research needs. To best use ASCR HPC resources the experimental HEP program needs a) an established long-term plan for access to ASCR computational and data resources, b) an ability to map workflows onto HPC resources, c) the ability for ASCR facilities to accommodate workflows run by collaborations that can have thousands of individual members, d) to transition codes to the next-generation HPC platforms that will be available at ASCR facilities, e) to build up and train a workforce capable of developing and using simulations and analysis to support HEP scientific research on next-generation systems.

  8. ASCR/HEP Exascale Requirements Review Report

    OpenAIRE

    Habib, S; Roser, R; Gerber, R; Antypas, K; Riley, K; Williams, T; Wells, J; Straatsma, T; Almgren, A; Amundson, J; Bailey, S; Bard, D; Bloom, K; Bockelman, B; Borgland, A

    2017-01-01

    This draft report summarizes and details the findings, results, and recommendations derived from the ASCR/HEP Exascale Requirements Review meeting held in June, 2015. The main conclusions are as follows. 1) Larger, more capable computing and data facilities are needed to support HEP science goals in all three frontiers: Energy, Intensity, and Cosmic. The expected scale of the demand at the 2025 timescale is at least two orders of magnitude -- and in some cases greater -- than that available c...

  9. HEP.TrkX [Vidyo

    CERN Multimedia

    CERN. Geneva

    2017-01-01

    Reconstruction of charged particle tracks is a central task in the processing of physics data at the LHC and other colliders. Current state-of-the-art tracking algorithms are based on the Kalman filter and have seen great success both offline and at trigger level. However, these algorithms scale poorly with increasing detector occupancy, and it is foreseen that significant changes will be needed to achieve efficient track reconstruction in very high luminosity conditions. The HEP.TrkX pilot project aims to develop and explore machine-learning-based algorithms for particle tracking, with the goal of identifying candidate techniques for a more scalable tracking algorithm. In this talk we will discuss the techniques explored in the project so far, with emphasis on algorithms based on recurrent and convolutional neural networks. We will demonstrate the performance of these algorithms on toy detector data, and discuss plans to adapt them into complete algorithms for seed-finding and/or full track reconstructio...

  10. Cross-national research on contractor evaluation procedures in public works procurement

    Science.gov (United States)

    Kinoshita, Seiya; Sato, Naoyoshi; Matsumoto, Naoya

    Contractor evaluation methods in Japan's public works procurement, beginning with construction business licensure, going through biennial preliminary firm rating, up to project-by-project prequalification and comprehensive point rating, were developed during the period when public works were mostly procured through designated competitive bidding. It is essential to focus attention on contractor evaluation methods for introducing different types of procurement procedures which enhance the use of technological capabilities held by private businesses. An overall review of contractor evaluation procedures should be conducted in view of the present situation, where the open competitive bidding has become mainly used in combination with comprehensive evaluation, as well as to allow for further diversification of procurement methods. In Western countries, improvements have been made for the past several years in contractor evaluation procedures with more emphasis on "Value for Money." Advanced efforts made by these countries will be useful as a reference for overhauling Japan's contractor evaluation system. This study conducts a comparative review of contractor evaluation procedures for public procurement in Western countries such as the United States, the United Kingdom and France by identifying similarities and differences between those of Japan and the above mentioned countries. This reveals that a contractor's technical or professional ability is looked at separately from its economic and financial standing in those countries studied, and there is no case like Japan in which those two factors are integrated into one for evaluation.

  11. Evaluation of a procedure to assess the adverse effects of illicit drugs.

    NARCIS (Netherlands)

    Amsterdam, J G C van; Best, W; Opperhuizen, A; Wolff, F A de

    2004-01-01

    The assessment procedure of new synthetic illicit drugs that are not documented in the UN treaty on psychotropic drugs was evaluated using a modified Electre model. Drugs were evaluated by an expert panel via the open Delphi approach, where the written score was discussed on 16 items, covering

  12. Evaluation of Approval Request 47; Call for Release Procedures for Charlotte Douglas International Airport

    Science.gov (United States)

    Stevens, Lindsay; Martin, Lynne; Jobe, Kimberly; Parke, Bonny; Chevalley, Eric; Lee, Hanbong; Verma, Savita; Dulchinos, Victoria

    2017-01-01

    NASA is collaborating with the Federal Aviation Administration (FAA) and aviation industry partners to develop and demonstrate new concepts and technologies for Integrated Arrival, Departure, and Surface (IADS) traffic management capabilities under the Airspace Technology Demonstration 2 (ATD-2) project. One of the goals of the IADS capabilities in the ATD-2 project is to increase predictability and throughput of airspace operations by improving Traffic Management Initiative (TMI) compliance. This paper focuses on the Approval Request (APREQ) procedures developed for the ATD-2 project between the Air Traffic Control (ATC) Tower at Charlotte Douglas International Airport and Washington Center. In March 2017, NASA conducted a Human-in-the-Loop (HITL) simulation to evaluate the operational procedures and information requirements for the APREQ procedures in the ATD-2 IADS system between ATC Tower and Center. The findings from the HITL are used to compare ATD-2 APREQ procedures with information about current day APREQ procedures.

  13. EVALUATION OF OUTCOME OF VARIOUS SURGICAL PROCEDURES FOR UPPER EYELID PTOSIS

    Directory of Open Access Journals (Sweden)

    Nagaraju

    2015-02-01

    Full Text Available INTRODUCTION: There are various procedures available for ptosis correction. Successful outcome not only depends on correct technique but also choosing appropriate procedure for each patient. Selection of procedure is based on available levator function and also other factors like etiology, severity, Bell’s phenomenon etc. If such varied procedure s are performed in a group of patients based on standard criteria and results are evaluated systematically we can determine what works best for a given patient . AIM : Evaluation of outcome of various surgical procedures for upper eyelid ptosis . METHODOLOGY : 25 eyelids of 20 patients who presented to a tertiary centre in south India with complaint of drooping of upper lid were considered. All subjects underwent complete ocular examination corrected visual acuity and detailed ptosis evaluation with particular emphasis on measurement of levator muscle function, MRD1 (margin reflex distance - 1, palpebral fissure width in different gazes and margin crease distance. The effect of various factors like MRD1, MCD, levator function were assessed, the amount of correction required and appropriate surgical procedure was chosen. Surgical procedure of Levator resection, frontalis sling operation, anterior levator aponeurosis advancement, o r other ptosis correction procedures under appropriate anaesthesia were performed. Post - operative evaluation in terms of visual acuity, MRD, Interpalpebral fissure height, lid symmetry, lagophthalmos and complications (if any was done. RESULTS : Levator muscle resection was done in 28% of eyelids, frontal sling surgery in 60% of eyelids, Levator muscle plication in 8% eyelids and levator muscle disinsertion with frontal sling surgery in 4% eyes. Undercorrection was seen in about 44% of eyelids in varying degrees. 56% of the eyes had optimal correction. Symmetric correction was achieved in 76% of eyelids. CONCLUSION: The influence of various preoperative factors on the

  14. The medical practice of euthanasia in Belgium and The Netherlands: legal notification, control and evaluation procedures.

    Science.gov (United States)

    Smets, Tinne; Bilsen, Johan; Cohen, Joachim; Rurup, Mette L; De Keyser, Els; Deliens, Luc

    2009-05-01

    To describe and compare current legal procedures for notifying, controlling and evaluating (NCE-procedures) euthanasia in Belgium and the Netherlands, and to discuss the implications for a safe and controllable euthanasia practice. We systematically studied and compared official documents relating to the Belgian and the Dutch NCE-procedures for euthanasia. In both countries, physicians are required to notify their cases to a review Committee, stimulating them to safeguard the quality of their euthanasia practice and to make societal control over the practice of euthanasia possible. However, the procedures in both countries differ. The main differences are that the Dutch notification and control procedures are more elaborate and transparent than the Belgian, and that the Belgian procedures are primarily anonymous, whereas the Dutch are not. Societal evaluation is made in both countries through the Committees' summary reports to Parliament. Transparent procedures like the Dutch may better facilitate societal control. Informing physicians about the law and the due care requirements for euthanasia, and systematic feedback about their medical actions are both pivotal to achieving efficient societal control and engendering the level of care needed when performing such far-reaching medical acts.

  15. Cytotoxic effects of etephon and maleic hydrazide in Vero, Hep2, HepG2 cells.

    Science.gov (United States)

    Yurdakok, Begum; Baydan, Emine; Okur, Hamza; Gurcan, Ismayil Safa

    2014-10-01

    The toxicity of etephon and maleic hydrazide, used as plant growth regulators in agriculture, were reported as low in mammals in previous studies. However, in vitro cytotoxicity studies in mammalian cells are currently missing to understand their toxicity at molecular level. In the current study, the cytotoxicity of these compounds, were studied in Vero (African green monkey kidney epithelium), HepG2 (human hepatocellular carcinoma), Hep2 (human epidermoid cancer) cells by MTT ((3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromure) and LDH (lactate dehydrogenase) assays. Maleic hydrazide had lower IC50 values for all cell lines compared to ethephon. Least cytotoxic effect treated by ethephon were observed in Vero, followed by HepG2 and Hep2. Similarly maleic hydrazide also showed least cytotoxicity on Vero cells, followed by Hep2 and HepG2 cells (p Vero cells, followed by HepG2 and Hep2 cells (p 0.868 (p cells to be supplemented by further studies.

  16. Evaluation of the sustainability of contrasted pig farming systems: the procedure, the evaluated systems and the evaluation tools.

    Science.gov (United States)

    Bonneau, M; de Greef, K; Brinkman, D; Cinar, M U; Dourmad, J Y; Edge, H L; Fàbrega, E; Gonzàlez, J; Houwers, H W J; Hviid, M; Ilari-Antoine, E; Klauke, T N; Phatsara, C; Rydhmer, L; van der Oever, B; Zimmer, C; Edwards, S A

    2014-12-01

    Although a few studies consider the sustainability of animal farming systems along the three classical main pillars (economy, environment and society), most studies on pig farming systems address only one of these pillars. The present paper is the introduction to a series of companion papers presenting the results of a study undertaken within the EU-supported project Q-PorkChains, aiming at building a comprehensive tool for the evaluation of pig farming systems, which is robust to accommodate the large variability of systems existing in Europe. The tool is mostly based on questions to farmers and comprises a total of 37 dimensions distributed along eight themes: Animal Welfare, Animal Health, Breeding Programmes, Environmental Sustainability, Meat Safety, Market Conformity, Economy and Working Conditions. The paper describes the procedure that was used for building the tool, using it on 15 contrasted pig farming systems and analysing the results. The evaluated systems are briefly described and a short overview of the dimensions is provided. Detailed descriptions of the theme-wise tools and results, as well as the results of an integrated evaluation, are available in the companion papers.

  17. Test and evaluation procedures for Sandia's Teraflops Operating System (TOS) on Janus.

    Energy Technology Data Exchange (ETDEWEB)

    Barnette, Daniel Wayne

    2005-10-01

    This report describes the test and evaluation methods by which the Teraflops Operating System, or TOS, that resides on Sandia's massively-parallel computer Janus is verified for production release. Also discussed are methods used to build TOS before testing and evaluating, miscellaneous utility scripts, a sample test plan, and a proposed post-test method for quickly examining the large number of test results. The purpose of the report is threefold: (1) to provide a guide to T&E procedures, (2) to aid and guide others who will run T&E procedures on the new ASCI Red Storm machine, and (3) to document some of the history of evaluation and testing of TOS. This report is not intended to serve as an exhaustive manual for testers to conduct T&E procedures.

  18. Application of the cloze procedure to evaluate comprehension and demonstrate rewriting of pharmacy educational materials.

    Science.gov (United States)

    Miller, Michael J; DeWitt, Jane E; McCleeary, Erin M; O'Keefe, Kelly J

    2009-04-01

    Written materials are commonly used to communicate pharmacy-relevant information to patients. However, they are often composed at a level that limits comprehension, mitigating a well-intended effect. To (1) use the cloze procedure (a test designed to assess reading comprehension) to evaluate an individual's understanding of a pharmacy-relevant educational pamphlet; (2) compare results of the cloze procedure with the reading comprehension component of the Short Test of Functional Health Literacy in Adults (S-TOFHLA); and (3) use results to demonstrate rewriting of the educational pamphlet. The cloze procedure was applied to a pharmacy-relevant educational pamphlet describing safe medication practices. A total of 162 subjects were recruited from university faculty, staff, and students; a local adult literacy center; and community senior centers. Subjects completed a background interview, the S-TOFHLA, and cloze procedure for the pharmacy-relevant educational pamphlet. S-TOFHLA and cloze procedure scores were described and compared. Cloze procedure responses were used to demonstrate revision of the pamphlet. Of the 154 subjects analyzed, mean +/- SD age was 56.5 +/- 20.4 years. Subjects were predominantly white (93.5%), female (71.4%), and college graduates (42.2%). Mean score on the S-TOFHLA was 92.1%. A majority (95.5%, 147/154) of subjects demonstrated adequate functional health literacy. In contrast, mean score on the cloze procedure was 53.3%. Internal consistencies of the S-TOFHLA and the cloze procedure were 0.92 and 0.90, respectively. Scores on the cloze procedure and the S-TOFHLA were highly correlated (r = 0.71, p educated, health-literate sample, a majority did not understand the pharmacy-relevant educational pamphlet despite adequate performance on a standard measure of health literacy. The cloze procedure can be used to assess comprehension of educational materials, solicit feedback from intended users, and guide the revision of educational materials.

  19. The HEP Software and Computing Knowledge Base

    Science.gov (United States)

    Wenaus, T.

    2017-10-01

    HEP software today is a rich and diverse domain in itself and exists within the mushrooming world of open source software. As HEP software developers and users we can be more productive and effective if our work and our choices are informed by a good knowledge of what others in our community have created or found useful. The HEP Software and Computing Knowledge Base, hepsoftware.org, was created to facilitate this by serving as a collection point and information exchange on software projects and products, services, training, computing facilities, and relating them to the projects, experiments, organizations and science domains that offer them or use them. It was created as a contribution to the HEP Software Foundation, for which a HEP S&C knowledge base was a much requested early deliverable. This contribution will motivate and describe the system, what it offers, its content and contributions both existing and needed, and its implementation (node.js based web service and javascript client app) which has emphasized ease of use for both users and contributors.

  20. Evaluation by policy makers of a procedure to describe perceived landscape openness.

    Science.gov (United States)

    Weitkamp, Gerd; Van den Berg, Agnes E; Bregt, Arnold K; Van Lammeren, Ron J A

    2012-03-01

    In the last decade policy makers have increasingly recognized the need to include people's perceptions in methods for describing landscape quality. At the same time, a third wave of Geographic Information Systems (GIS) has become available that make it technically possible to model landscape quality in a realistic manner. However, as there is often a mismatch between science and policy, it remains unclear to what extent perception-based models developed by scientists can be useful to policy makers. The aim of the present study was to evaluate the usefulness to policy making of a GIS-based procedure for describing perceived landscape openness. To this end, a workshop was organized which was attended by eight Dutch policy makers who acted as representatives of their province (region). The Group Decision Room (GDR) technique was used to elicit the policy makers' evaluations of the procedure in an anonymous and reliable manner. The procedure was presented to the policy makers using cases from their own province, which they assessed using a mixture of qualitative and quantitative methods. The results show that policy makers rated the procedure as being highly relevant to policy making, scientifically credible, usable by policy makers and feasible to implement in the policy making process. They especially appreciated the flexibility and transparency of the procedure. The policy makers concluded that the procedure would be of most value for monitoring landscape changes and for analysing impacts on landscape openness in land use scenario studies. However, they requested guidelines for proper implementation of the various options in the procedure. In general, the current study shows that explicit and transparent evaluation of the usefulness of GIS-based tools can aid integration at the science-policy interface and help to ensure that both scientists and policy makers are informed of interrelated options and requirements. Copyright © 2011 Elsevier Ltd. All rights reserved.

  1. ASCR/HEP Exascale Requirements Review Report

    CERN Document Server

    Habib, Salman; Gerber, Richard; Antypas, Katie; Riley, Katherine; Williams, Tim; Wells, Jack; Straatsma, Tjerk; Almgren, A; Amundson, J; Bailey, S; Bard, D; Bloom, K; Bockelman, B; Borgland, A; Borrill, J; Boughezal, R; Brower, R; Cowan, B; Finkel, H; Frontiere, N; Fuess, S; Ge, L; Gnedin, N; Gottlieb, S; Gutsche, O; Han, T; Heitmann, K; Hoeche, S; Ko, K; Kononenko, O; LeCompte, T; Li, Z; Lukic, Z; Mori, W; Nugent, P; Ng, C -K; Oleynik, G; O'Shea, B; Padmanabhan, N; Petravick, D; Petriello, F J; Power, J; Qiang, J; Reina, L; Rizzo, T J; Ryne, R; Schram, M; Spentzouris, P; Toussaint, D; Vay, J -L; Viren, B; Wurthwein, F; Xiao, L

    2016-01-01

    This draft report summarizes and details the findings, results, and recommendations derived from the ASCR/HEP Exascale Requirements Review meeting held in June, 2015. The main conclusions are as follows. 1) Larger, more capable computing and data facilities are needed to support HEP science goals in all three frontiers: Energy, Intensity, and Cosmic. The expected scale of the demand at the 2025 timescale is at least two orders of magnitude -- and in some cases greater -- than that available currently. 2) The growth rate of data produced by simulations is overwhelming the current ability, of both facilities and researchers, to store and analyze it. Additional resources and new techniques for data analysis are urgently needed. 3) Data rates and volumes from HEP experimental facilities are also straining the ability to store and analyze large and complex data volumes. Appropriately configured leadership-class facilities can play a transformational role in enabling scientific discovery from these datasets. 4) A c...

  2. DD4Hep based event reconstruction

    CERN Document Server

    AUTHOR|(SzGeCERN)683529; Frank, Markus; Gaede, Frank-Dieter; Hynds, Daniel; Lu, Shaojun; Nikiforou, Nikiforos; Petric, Marko; Simoniello, Rosa; Voutsinas, Georgios Gerasimos

    The DD4HEP detector description toolkit offers a flexible and easy-to-use solution for the consistent and complete description of particle physics detectors in a single system. The sub-component DDREC provides a dedicated interface to the detector geometry as needed for event reconstruction. With DDREC there is no need to define an additional, separate reconstruction geometry as is often done in HEP, but one can transparently extend the existing detailed simulation model to be also used for the reconstruction. Based on the extension mechanism of DD4HEP, DDREC allows one to attach user defined data structures to detector elements at all levels of the geometry hierarchy. These data structures define a high level view onto the detectors describing their physical properties, such as measurement layers, point resolutions, and cell sizes. For the purpose of charged particle track reconstruction, dedicated surface objects can be attached to every volume in the detector geometry. These surfaces provide the measuremen...

  3. Integrating Program Theory and Systems-Based Procedures in Program Evaluation: A Dynamic Approach to Evaluate Educational Programs

    Science.gov (United States)

    Grammatikopoulos, Vasilis

    2012-01-01

    The current study attempts to integrate parts of program theory and systems-based procedures in educational program evaluation. The educational program that was implemented, called the "Early Steps" project, proposed that physical education can contribute to various educational goals apart from the usual motor skills improvement. Basic…

  4. Procedural Principles of Design : The Role of Analysis and Evaluation in Educational Design Processes

    NARCIS (Netherlands)

    Visscher-Voerman, Irene

    2007-01-01

    Educational designers conduct a variety of design activities in practice. Building on several reconstructive studies of design practice, 8 procedural design principles, related to analysis and formative evaluation activities were formulated and discussed and elaborated in a one-day workshop with 24

  5. A National Survey of Faculty Development Evaluation Outcome Measures and Procedures

    Science.gov (United States)

    Meyer, Katrina A.; Murrell, Vicki S.

    2014-01-01

    This article presents the results of a national study of 39 higher education institutions that collected information about their evaluation procedures and outcome measures for faculty development for online teaching conducted during 2011-2012. The survey results found that over 90% of institutions used measures of the faculty person's…

  6. Data formats and procedures for the Evaluated Nuclear Data File, ENDF

    Energy Technology Data Exchange (ETDEWEB)

    Garber, D.; Dunford, C.; Pearlstein, S.

    1975-10-01

    This report describes the philosophy of the Evaluated Nuclear Data File (ENDF) and the data formats and procedures that have been developed for it. The ENDF system was designed for the storage and retrieval of the evaluated nuclear data that are required for neutronics, photonics and decay heat calculations. This system is composed of several parts that include a series of data processing codes and neutron and photon cross section nuclear structure libraries.

  7. Evaluation of Hands-Free Devices for the Display of Maintenance Procedures

    Science.gov (United States)

    Whitmore, Mihriban; Hoffman, Ronald B.; Litaker, Harry, Jr.; Solem, Jody; Holden, Kritina; Twyford, Evan; Conlee, Carl

    2007-01-01

    Over the past year, NASA's focus has turned to crewed long duration and exploration missions. On these journeys, crewmembers will be required to execute thousands of procedures to maintain life support systems, check out space suits, conduct science experiments, and perform medical exams. To support the many complex tasks crewmembers undertake in microgravity, NASA is interested in providing crewmembers a hands-free work environment to promote more efficient operations. The overarching objective is to allow crewmembers to use both of their hands for tasks related to their mission, versus holding a paper manual or interacting with a display. The use of advanced, hands-free tools will undoubtedly make the crewmembers task easier, but they can also add to overall task complexity if not properly designed. A leading candidate technology for supporting a hands-free environment is the Head-Mounted Display (HMD). A more recent technology (e-book reader) that could be easily temp-stowed near the work area is also a potential hands-free solution. Previous work at NASA involved the evaluation of several commercially available HMDs for visual quality, comfort, and fit, as well as suitability for use in microgravity. Based on results from this work, three HMDs were selected for further evaluation (along with an e-book reader), using International Space Station (ISS)-like maintenance procedures. Two evaluations were conducted in the Space Station Mockup and Trainer Facility (SSMTF) located at the NASA Johnson Space Center (building 9). The SSMTF is a full scale, medium fidelity replica of the pressurized portions of the ISS. It supports crew training such as ingress and egress, habitability, and emergency procedures. In each of the two evaluations, the participants performed two maintenance procedures. One maintenance procedure involved inspecting air filters in a life support system and replacing them with a clean filter if one were found to be contaminated. The second

  8. CompHEP: developments and applications

    Science.gov (United States)

    Boos, E. E.; Bunichev, V. E.; Dubinin, M. N.; Ilyin, V. A.; Savrin, V. I.; CompHEP Collaboration

    2017-11-01

    New developments of the CompHEP package and its applications to the top quark and the Higgs boson physics at the LHC collider are reviewed. These developments were motivated mainly by the needs of experimental searches of DO (Tevatron) and CMS (LHC) collaborations where identification of the top quark and the Higgs boson in the framework of the Standard Model (SM) or possible extensions of the SM played an important role. New useful features of the CompHEP Graphics User Interface (GUI) are described.

  9. Avaliação da capacidade física e do estado nutricional em candidatos ao transplante hepático Evaluation of the nutritional status and physical performance in candidates to liver transplantation

    Directory of Open Access Journals (Sweden)

    Ana V. A. Leitão

    2003-01-01

    Full Text Available OBJETIVO: Avaliar o estado nutricional, os indicadores de capacidade física e suas relações com a função hepática, em pacientes candidatos ao transplante de fígado. MÉTODOS: Foram estudados 32 pacientes pré-transplante, classificados como Child-Pugh A (25%, B (22% e C (53%. A capacidade física foi avaliada pelos parâmetros: força muscular do quadríceps, dos flexores plantares e dorsais do pé; equilíbrio estático; coordenação; velocidade da marcha; teste de 6 minutos de caminhada e teste "timed get up and go"; índices de Barthel e de Karnofsky. O estado nutricional foi avaliado pelo escore de Mendenhall e classificado segundo Blackburn. RESULTADOS: Foi detectado déficit de força de quadríceps (32% dos pacientes, do equilíbrio (34,8%, da velocidade da marcha (66,7% e da coordenação (15,6%. O teste de caminhada indicou baixo desempenho físico em 72,5 % dos pacientes (moderado em 40,9% e grave em 31,6%. O índice de Karnofsky revelou que 43,8% dos pacientes necessitava assistência considerável. Foi identificada desnutrição em 62,5% dos pacientes (leve em 28,13%; moderada e grave em 34,37%. O teste do qui quadrado não mostrou associação entre a classificação de Child-Pugh e o estado nutricional, o teste de caminhada e o índice de Karnofsky. A associação entre o teste de caminhada e o índice de Karnofsky foi altamente significante (pOBJECTIVE: To evaluate the physical capacity and nutritional status of patients before orthotopic liver transplantation (OLT and to correlate these parameters to the severity of liver function. METHODS: Thirty-two patients before OTL were classified as Child-Pugh A (25%, B (22% and C (53%. Physical capacity was analyzed by the quadriceps and ankle strength, balance, coordination, gait velocity, 6 minute walk test, get up and go test, Barthel and Karnofsky indexes. Nutritional status was evaluated by using Mendenhall score and Blackburn classification. RESULTS: Quadriceps weakness was

  10. A simplified LBB evaluation procedure for austenitic and ferritic steel piping

    Energy Technology Data Exchange (ETDEWEB)

    Gamble, R.M.; Wichman, K.R.

    1997-04-01

    The NRC previously has approved application of LBB analysis as a means to demonstrate that the probability of pipe rupture was extremely low so that dynamic loads associated with postulated pipe break could be excluded from the design basis (1). The purpose of this work was to: (1) define simplified procedures that can be used by the NRC to compute allowable lengths for circumferential throughwall cracks and assess margin against pipe fracture, and (2) verify the accuracy of the simplified procedures by comparison with available experimental data for piping having circumferential throughwall flaws. The development of the procedures was performed using techniques similar to those employed to develop ASME Code flaw evaluation procedures. The procedures described in this report are applicable to pipe and pipe fittings with: (1) wrought austenitic steel (Ni-Cr-Fe alloy) having a specified minimum yield strength less than 45 ksi, and gas metal-arc, submerged arc and shielded metal-arc austentic welds, and (2) seamless or welded wrought carbon steel having a minimum yield strength not greater than 40 ksi, and associated weld materials. The procedures can be used for cast austenitic steel when adequate information is available to place the cast material toughness into one of the categories identified later in this report for austenitic wrought and weld materials.

  11. Application and Evaluation of an Expert Judgment Elicitation Procedure for Correlations

    Science.gov (United States)

    Zondervan-Zwijnenburg, Mariëlle; van de Schoot-Hubeek, Wenneke; Lek, Kimberley; Hoijtink, Herbert; van de Schoot, Rens

    2017-01-01

    The purpose of the current study was to apply and evaluate a procedure to elicit expert judgments about correlations, and to update this information with empirical data. The result is a face-to-face group elicitation procedure with as its central element a trial roulette question that elicits experts' judgments expressed as distributions. During the elicitation procedure, a concordance probability question was used to provide feedback to the experts on their judgments. We evaluated the elicitation procedure in terms of validity and reliability by means of an application with a small sample of experts. Validity means that the elicited distributions accurately represent the experts' judgments. Reliability concerns the consistency of the elicited judgments over time. Four behavioral scientists provided their judgments with respect to the correlation between cognitive potential and academic performance for two separate populations enrolled at a specific school in the Netherlands that provides special education to youth with severe behavioral problems: youth with autism spectrum disorder (ASD), and youth with diagnoses other than ASD. Measures of face-validity, feasibility, convergent validity, coherence, and intra-rater reliability showed promising results. Furthermore, the current study illustrates the use of the elicitation procedure and elicited distributions in a social science application. The elicited distributions were used as a prior for the correlation, and updated with data for both populations collected at the school of interest. The current study shows that the newly developed elicitation procedure combining the trial roulette method with the elicitation of correlations is a promising tool, and that the results of the procedure are useful as prior information in a Bayesian analysis. PMID:28197115

  12. Involvement of the multidrug resistance P-glycoprotein in acetaminophen-induced toxicity in hepatoma-derived HepG2 and Hep3B cells.

    Science.gov (United States)

    Manov, Irena; Bashenko, Yulia; Hirsh, Mark; Iancu, Theodore C

    2006-09-01

    Acetaminophen overdose causes severe hepatic failure. Although the mechanisms of acetaminophen hepatotoxicity have been well investigated, little is known about the involvement of the P-glycoprotein in acetaminophen transport and toxicity. P-Glycoprotein is a membrane efflux pump, playing a significant role in regulating absorption, excretion, and tissue distribution of many drugs. To evaluate the contribution of P-glycoprotein transporter in the course of acetaminophen-induced toxicity, HepG2 and Hep3B cells with different P-glycoprotein expression and activity, were treated by acetaminophen (1-10 mM) for different time periods, with or without the P-glycoprotein inhibitor verapamil. P-Glycoprotein activity was determined by rhodamine 123 efflux assay and western blot analysis. To assess the acetaminophen-induced toxicity and effect of verapamil, we investigated cellular redox status, phosphatidylserine externalization, nuclear fragmentation and ultrastructural changes. Verapamil markedly enhanced acetaminophen-induced oxidative damage and cell death. Moreover, verapamil revealed acetaminophen toxicity even at subtoxic levels. High acetaminophen concentrations increased P-glycoprotein activity and content in both HepG2 and Hep3B cells. These observations suggest the involvement of P-glycoprotein in acetaminophen transport. Notwithstanding the differences of the investigated hepatoma cell lines in P-glycoprotein function, acetaminophen-induced toxicity was similar, possibly due to different functions of drug-metabolizing systems. We conclude that acetaminophen is a P-glycoprotein substrate and P-glycoprotein is involved in acetaminophen transport and toxicity in HepG2 and Hep3B cells. This study establishes the fact that acetaminophen can modulate P-glycoprotein in tumour cells, suggesting that its routine use in cancer patients in combination with anticancer drugs, may influence the result of chemotherapy.

  13. Immunogenicity and safety study of a new DTaP-IPV-Hep B-PRP-T combined vaccine compared to a licensed DTaP-IPV-Hep B//PRP-T comparator, both concomitantly administered with a 7-valent pneumococcal conjugate vaccine at 2, 4, and 6 months of age in Thai infants.

    Science.gov (United States)

    Kosalaraksa, Pope; Thisyakorn, Usa; Benjaponpitak, Suwat; Chokephaibulkit, Kulkanya; Santos-Lima, Eduardo

    2011-04-01

    To assess a new, fully-liquid, hexavalent DTaP-IPV-Hep B-PRP-T vaccine (diphtheria toxoid (D), tetanus toxoid (T), acellular pertussis (aP), inactivated poliovirus (IPV), hepatitis B (Hep B), and Haemophilus influenzae type b polysaccharide conjugated to tetanus protein (PRP-T) antigens) compared to a licensed DTaP-IPV-Hep B//PRP-T vaccine following primary series co-administration with a 7-valent pneumococcal conjugate vaccine (PCV7). This was a randomized, phase III, observer-blind study in Thai infants (N=412), who received DTaP-IPV-Hep B-PRP-T or DTaP-IPV-Hep B//PRP-T at 2, 4, and 6 months of age, co-administered with PCV7. All received Hep B at birth. Non-inferiority for Hep B ≥ 10 mIU/ml and PRP ≥0.15μg/ml was analyzed (DTaP-IPV-Hep B-PRP-T relative to DTaP-IPV-Hep B//PRP-T) at 1 month post-primary. Seroprotection/seroconversion and geometric mean titers (GMTs) were analyzed descriptively for all hexavalent components. Safety was evaluated from parental reports. Anti-Hep B and anti-PRP antibody seroprotection rates were high for DTaP-IPV-Hep B-PRP-T (n=189) and DTaP-IPV-Hep B//PRP-T (n=190), and non-inferiority was demonstrated. Anti-D and anti-T ≥ 0.01 IU/ml, anti-polio types 1, 2, and 3 ≥ 8 (1/dil), and anti-PT and anti-FHA seroconversion were high and similar in each group. For DTaP-IPV-Hep B-PRP-T and DTaP-IPV-Hep B//PRP-T, anti-Hep B ≥ 100 mIU/ml was 98.4% and 99.5% (GMTs 2477 and 2442 mIU/ml), respectively; anti-PRP ≥ 1.0 μg/ml was 85.2% and 71.1% (GMTs 5.07 and 2.41 μg/ml), respectively. Safety profiles were comparable. There were no vaccine-related serious adverse events. Following co-administration with PCV7 the investigational DTaP-IPV-Hep B-PRP-T vaccine was safe and immunogenic. Non-inferiority to DTaP-IPV-Hep B//PRP-T was shown for Hep B and PRP. Copyright © 2011 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  14. Processing Contexts for Experimental HEP Data

    Energy Technology Data Exchange (ETDEWEB)

    Paterno, Marc [Fermilab; Green, Chris [Fermilab

    2017-02-06

    This document provides, for those not closely associated with the experimental High Energy Physics (HEP) community, an introduction to data input and output requirements for a variety of data processing tasks. Examples in it are drawn from the art event processing framework, and from experiments and projects using art, most notably the LArSoft and NuTools projects.

  15. HEP meets ML award talk : XGBoost

    CERN Multimedia

    CERN. Geneva; CHEN, Tianqi

    2015-01-01

    Tianqi Chen and Tong He (team crowwork) have provided very early in the challenge to all participants XGBoost (for eXtreme Gradient Boosted). It is a parallelised software to train boost decision trees, which has been effectively used by many participants to the challenge. For this, they have won the "HEP meets ML" award which is the invitation to CERN happening today.

  16. Implementation of the HEPS Schoolkit: initial steps

    DEFF Research Database (Denmark)

    Simovska, Venka; Woynarovska, Barbara; Samdal, Oddrun

    This report is an output of one of the work packages of the HEPS project (WP8, Research on Implementation). It is based on a survey administered with the SHE national coordinators, and additional interviews related to the survey. The purpose is to learn at early stages of implementation, to combine...

  17. The path toward HEP High Performance Computing

    CERN Document Server

    Apostolakis, John; Carminati, Federico; Gheata, Andrei; Wenzel, Sandro

    2014-01-01

    High Energy Physics code has been known for making poor use of high performance computing architectures. Efforts in optimising HEP code on vector and RISC architectures have yield limited results and recent studies have shown that, on modern architectures, it achieves a performance between 10% and 50% of the peak one. Although several successful attempts have been made to port selected codes on GPUs, no major HEP code suite has a 'High Performance' implementation. With LHC undergoing a major upgrade and a number of challenging experiments on the drawing board, HEP cannot any longer neglect the less-than-optimal performance of its code and it has to try making the best usage of the hardware. This activity is one of the foci of the SFT group at CERN, which hosts, among others, the Root and Geant4 project. The activity of the experiments is shared and coordinated via a Concurrency Forum, where the experience in optimising HEP code is presented and discussed. Another activity is the Geant-V project, centred on th...

  18. An Evaluation of Respondent Conditioning Procedures to Decrease Barking in an Animal Shelter

    Directory of Open Access Journals (Sweden)

    Steven Wade Payne

    2017-06-01

    Full Text Available A common problem behavior in animal shelters is excessive noise from barking, which can regularly exceed 100dBs. Noise levels in animal shelters are correlated with increased stress in dogs, which may lead to increased problem behavior and a decrease in adoption. The purpose of the current study was to evaluate the use of respondent conditioning procedures to reduce barking noise level in an animal shelter by pairing a door chime with edible items. Following a baseline and neutral stimulus phase, the door chime was paired with edible items over a period of three weeks. Following this pairing phase, the pairing was stopped to determine if the door chime would act as a conditioned stimulus and reduce barking. These procedures were replicated following an additional baseline phase. Overall, the procedure was effective in reducing the noise level of the kennel area as compared to baseline levels. Implications and future research areas are discussed

  19. Recursive Approach for Evaluation of Time Intervals between Transactions in Polling Procedure

    Directory of Open Access Journals (Sweden)

    Larkin Eugene

    2016-01-01

    Full Text Available An ergodic semi-Markov process with the structure represented by the full graph with loops, which simulates a digital control algorithm that generated transactions onto an object, is investigated. Elementary simplifications for reduction of semi-Markov processes are defined. Recursive procedure for reduction of initial semi-Markov process structure till the model, which includes selected states with its links only, is proposed. Formulae for recalculation of probabilities, weighted densities and expectations of time of switching to linked states are obtained. It is shown that recursive procedure may be used also for calculation of time expectation of return the process to one of selected states that simplified the task of evaluation of time intervals between transactions in polling procedure.

  20. Quantitative procedure for evaluation of microstructure of cast Mg-Al-Ca-Sr magnesium alloy

    Directory of Open Access Journals (Sweden)

    T. Rzychoń

    2010-01-01

    Full Text Available In this paper the microstructural characterization of ingot MRI-230D magnesium alloy and quantitative procedure for evaluation of microstructure are presented. The optical and scanning electron microscopy were used to study the morphology of microstructural compounds in this alloy. The X-ray diffraction was used to determination of phase composition. The as-cast microstructure of MRI-230D magnesium alloy containing aluminum, calcium and strontium consists of the dendritic α-Mg and such intermetallic compounds as: Al2Ca, Al4Sr and AlxMny. In the purpose quantitative description of microstructure semi-automatic procedures using Met-Ilo image analysis were developed. Prepared semi-automatic procedures allow a fast determination of phase content in MRI-230D alloy using light microscopy and will be useful in the quality control of MRI-230D ingots.

  1. Evaluation of the Prevalence of Different Treatment Failure Modes after Crown Lengthening Surgical Procedures

    Directory of Open Access Journals (Sweden)

    Amirreza Babaloo

    2016-07-01

    Full Text Available Introduction: Preservation of the health of periodontium is very important for the long-term success of restored teeth and a balance should always be created between the patients’ esthetic requirements and the periodontal health. Failures of crown lengthening procedures are classified into early and late failures. The aim of this study was to evaluate the prevalence of early failures of crown leathering surgical procedures. Materials and methods: In this descriptive/cross-sectional study, 96 patients were selected from those referring to the Department of Periodontitis, Tabriz Faculty of Dentistry, who required crown lengthening procedures. The particulars of these patients were recorded in special forms and the reasons for the failure of surgical procedures were separately determined at 2- and 6-week intervals. In addition, the frequencies of the reasons for failures were determined in percentages and absolute frequencies. Data were analyzed with descriptive statistics (frequencies and percentages using SPSS 21. Statistical significance was set at P<0.05. Results: Evaluation of patients 6 weeks after surgery showed a failure rate of 14.5% for crown lengthening procedures in patients referring to the Department of Periodontics, Tabriz Faculty of Dentistry. The most common reasons for such early failures in the 6th week, in descending order, were a lack of sufficient keratinized gingiva around in tooth in question, fracture of the tooth structure after surgery, inadequate surgery (not creating a proper distance between the healthy margin and the crest and the coronal returning of the gingival tissue on the tooth. A lack of sufficient keratinized gingiva around the tooth was the most frequent reason for the early failure of crown lengthening procedure at both study intervals. Conclusion: It can be concluded from the results of the present study that during the 6th postoperative week the crown lengthening procedures exhibited a 14.5% failure rate

  2. Usefulness of ultrasonography in follow-up evaluation after Ilizarov procedure : comparison with plain radiography

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jong Hoon; Jee, Sung Woo; Lee, Ho Won; Lee, Sung Moon; Kim, Jung Sik; Kim, Hong; Song, Kwang Soon; Woo, Sung Ku [Keimyung University, Taegu (Korea, Republic of). School of Medicine; Cho, Kil Ho [Yeungnam Univ., Taegu (Korea, Republic of). School of Medicine

    1998-06-01

    The purpose of this study is to compare the usefulness of ultrasonography in follow up evaluation after the Ilizarov procedure with that of plain radiography. We analyzed ultrasound findings in twelve patients who had undergone the Ilizarov procedure, and retrospectively compared the results with the findings of plain radiography. The procedure was performed due to post-traumatic limb deformity or non-union (nine of twelve patients), congenital pseudoarthrosis (one case), sequelae of poliomyelitis (one case), and short stature (one case). The patients` mean age was 29 years; eleven of twelve were male. Ten of twelve procedures were performed on the tibia and two on the femur. After 7-10 days, distraction was initiated at a rate of 0.25 mm four times a day. Ultrasonography was performed with a 5-10 MHz linear or convex transducer; new bone formation was defined as dotted or linear echogenic foci within a hypoechoic distraction gap. Initial ultrasonographic examination was performed 2-4 weeks after distraction, and the results were compared with those of plain radiography. Color doppler imaging was performed in three cases. After the Ilizarov bone lengthening procedure, ultrasound appears to be useful for follow-up examination. New bone formation, as well as complications, can be detected earlier; it can indicate whether ultrasound-guided interventional procedure is required, and if combined with color doppler study, is able to detect the periosteal blood supply. Further evaluation of the clinical significance of preserved periosteal blood supply seems however, to be needed. (author). 18 refs., 4 figs.

  3. Cytostatic activity of Geranium robertianum L. extracts processed by membrane procedures

    OpenAIRE

    Neagu, Elena; Paun, Gabriela; Constantin, Daniel; Radu, Gabriel Lucian

    2013-01-01

    In the present study the antioxidant and cytostatic capacities of some 8% Geranium robertianum (geranium) aqueous extracts processed by membrane procedures (micro- and ultrafiltration) were determined. The extracts were purified by microfiltration and then concentrated by successive ultrafiltrations using Millipore membranes with 10,000 and 1000 Da cut-off. Two methods were used to establish the extracts’ antioxidant activity: DPPH and ABTS; the cytostatic capacity was evaluated on HEp-2p cel...

  4. Continuously variable rating: a new, simple and logical procedure to evaluate original scientific publications

    Directory of Open Access Journals (Sweden)

    Mauricio Rocha e Silva

    2011-01-01

    Full Text Available OBJECTIVE: Impact Factors (IF are widely used surrogates to evaluate single articles, in spite of known shortcomings imposed by cite distribution skewness. We quantify this asymmetry and propose a simple computer-based procedure for evaluating individual articles. METHOD: (a Analysis of symmetry. Journals clustered around nine Impact Factor points were selected from the medical ‘‘Subject Categories’’ in Journal Citation Reports 2010. Citable items published in 2008 were retrieved and ranked by granted citations over the Jan/2008 - Jun/2011 period. Frequency distribution of cites, normalized cumulative cites and absolute cites/decile were determined for each journal cluster. (b Positive Predictive Value. Three arbitrarily established evaluation classes were generated: LOW (1.33.9. Positive Predictive Value for journal clusters within each class range was estimated. (c Continuously Variable Rating. An alternative evaluation procedure is proposed to allow the rating of individually published articles in comparison to all articles published in the same journal within the same year of publication. The general guiding lines for the construction of a totally dedicated software program are delineated. RESULTS AND CONCLUSIONS: Skewness followed the Pareto Distribution for (1procedure capable of accurately providing a valid rating for each article within the journal and time frame in which it was published.

  5. Evaluation of patient radiation doses using DAP meter in interventional radiology procedures

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Byung Sam [Dept. of Radiological Technology. Shingu University, Sungnam (Korea, Republic of); Yoon, Yong Su [Dept. of Health Sciences, Graduate School of Medical Sciences, Kyushu Univeristy, Kyushu (Japan)

    2017-03-15

    The author investigated interventional radiology patient doses in several other countries, assessed accuracy of DAP meters embedded in intervention equipment in domestic country, conducted measurement of patient doses for 13 major interventional procedures with use of Dose Area Product(DAP) meters from 23 hospitals in Korea, and referred to 8,415 cases of domestic data related to interventional procedures by radiation exposure after evaluation the actual effective of dose reduction variables through phantom test. Finally, dose reference level for major interventional procedures was suggested. In this study, guidelines for patient doses were 237.7 Gy·cm{sup 2} in TACE, 17.3 Gy·cm{sup 2} in AVF, 114.1 Gy·cm{sup 2} in LE PTA and STENT, 188.5 Gy·cm{sup 2} in TFCA, 383.5 Gy·cm{sup 2} in Aneurysm Coil, 64.6 Gy·cm{sup 2} in PTBD, 64.6 Gy·cm{sup 2} in Biliary Stent, 22.4 Gy·cm{sup 2} in PCN, 4.3 Gy·cm{sup 2} in Hickman, 2.8 Gy·cm{sup 2} in Chemo-port, 4.4 Gy·cm{sup 2} in Perm-Cather, 17.1 Gy·cm{sup 2} in PCD, and 357.9 Gy·cm{sup 2} in Vis, EMB. Dose reference level acquired in this study is considered to be able to use as minimal guidelines for reducing patient dose in the interventional radiology procedures. For the changes and advances of materials and development of equipment and procedures in the interventional radiology procedures, further studies and monitoring are needed on dose reference level Korean DAP dose conversion factor for the domestic procedures.

  6. Application and testing of a procedure to evaluate transferability of habitat suitability criteria

    Science.gov (United States)

    Thomas, Jeff A.; Bovee, Ken D.

    1993-01-01

    A procedure designed to test the transferability of habitat suitability criteria was evaluated in the Cache la Poudre River, Colorado. Habitat suitability criteria were developed for active adult and juvenile rainbow trout in the South Platte River, Colorado. These criteria were tested by comparing microhabitat use predicted from the criteria with observed microhabitat use by adult rainbow trout in the Cache la Poudre River. A one-sided X2 test, using counts of occupied and unoccupied cells in each suitability classification, was used to test for non-random selection for optimum habitat use over usable habitat and for suitable over unsuitable habitat. Criteria for adult rainbow trout were judged to be transferable to the Cache la Poudre River, but juvenile criteria (applied to adults) were not transferable. Random subsampling of occupied and unoccupied cells was conducted to determine the effect of sample size on the reliability of the test procedure. The incidence of type I and type II errors increased rapidly as the sample size was reduced below 55 occupied and 200 unoccupied cells. Recommended modifications to the procedure included the adoption of a systematic or randomized sampling design and direct measurement of microhabitat variables. With these modifications, the procedure is economical, simple and reliable. Use of the procedure as a quality assurance device in routine applications of the instream flow incremental methodology was encouraged.

  7. Proceduralism and its role in economic evaluation and priority setting in health.

    Science.gov (United States)

    Jan, Stephen

    2014-05-01

    This paper provides a critical overview of Gavin Mooney's proceduralist approach to economic evaluation and priority setting in health. Proceduralism is the notion that the social value attached to alternative courses of action should be determined not only by outcomes, but also processes. Mooney's brand of proceduralism was unique and couched within a broader critique of 'neo-liberal' economics. It operated on a number of levels. At the micro level of the individual program, he pioneered the notion that 'process utility' could be valued and measured within economic evaluation. At a macro level, he developed a framework in which the social objective of equity was defined by procedural justice in which communitarian values were used as the basis for judging how resources should be allocated across the health system. Finally, he applied the notion of procedural justice to further our understanding of the political economy of resource allocation; highlighting how fairness in decision making processes can overcome the sometimes intractable zero-sum resource allocation problem. In summary, his contributions to this field have set the stage for innovative programs of research to help in developing health policies and programs that are both in alignment with community values and implementable. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Avaliação dos potenciais evocados relacionados a eventos (ERP-P300 em pacientes com cirrose hepática sem encefalopatia Evaluation of the event-related potential (ERP-P300 in patients with hepatic cirrhosis without encephalopathy

    Directory of Open Access Journals (Sweden)

    Vinicius Teodoro

    2008-03-01

    Full Text Available RACIONAL: Na cirrose hepática ocorrem alterações na estrutura do fígado, levando à perda das funções do órgão, com conseqüências neuropsiquiátricas, como disfunções cognitivas. Um dos meios mais efetivos para avaliar objetivamente as funções cognitivas é medir a atividade eletrofisiológica do sistema nervoso central através do potencial evocado relacionado a eventos (ERP-P300. OBJETIVO: Estudar a utilidade do potencial evocado relacionado a eventos (ERP, para avaliar distúrbios cognitivos em pacientes com cirrose hepática e como auxiliar no diagnóstico da encefalopatia hepática mínima. MÉTODO: Foram selecionados 50 pacientes, diagnosticados com cirrose hepática sem sinais clínicos de encefalopatia hepática e 35 voluntários saudáveis de idades e sexo semelhantes. Em todos os pacientes foram realizados exames clínico-neurológico e laboratoriais. Para identificação do prejuízo cognitivo foi utilizado o ERP-P300 e obtida a média da latência da onda P300. RESULTADOS: Houve diferença significativa entre as médias da latência do ERP-P300 do grupo cirrótico e controle. CONCLUSÃO: A realização do ERP-P300 é simples, depende de fatores controláveis de variação e tem fácil reprodutibilidade, podendo ser útil para o rastreio de distúrbios cognitivos em pacientes com cirrose e auxiliar no diagnóstico de encefalopatia hepática mínima.BACKGROUND: In hepatic cirrhosis structural liver alterations occur leading to the loss of the organ functions with neuro-psychiatric consequence, as cognitive dysfunctions. One of the most effective ways of objectively evaluating cognition is to measure electrophysiological activity in the central nervous system trough event-related potentials (ERP-P300. AIM: To assess the value of the event-related potential (ERP in order to determine cognitive disturbances in patients with liver cirrhosis and to assist in the diagnosis of minimal hepatic encephalopathy. METHODS: Fifty

  9. Selective effects of a fiber chimeric conditionally replicative adenovirus armed with hep27 gene on renal cancer cell.

    Science.gov (United States)

    Fang, Lin; Cheng, Qian; Liu, Wenshun; Zhang, Jie; Ge, Yan; Zhang, Qi; Li, Liantao; Liu, Junjie; Zheng, Junnian

    2016-06-02

    ASBTARCT Adenoviruses mediated cancer gene therapies are widely investigated and show a promising effect on cancer treatment. However, efficient gene transfer varies among different cancer cell lines based on the expression of coxsakie adenovirus receptor (CAR). Hep27, a member of dehydrogenase/reductase (SDR) family, can bind to Mdm2, resulting in the attenuation of Mdm2-mediated p53 degradation. Here we constructed a fiber chimeric adenovirus carrying hep27 gene (F5/35-ZD55-Hep27), in which the fiber protein of 5-serotype adenovirus (Ad5) was substituted by that of 35-serotype adenovirus (Ad35), aiming to facilitate the infection for renal cancer cells and develop the role of hep27 in cancer therapy. We evaluated the CAR and CD46 (a membrane cofactor protein for Ad35) expression in four kinds of renal cancer cells and assessed the relationship between receptors and infection efficiency. 5/35 fiber-modified adenovirus had a much promising infectivity compared with Ad5-based vector in renal cancer cells. F5/35-ZD55-Hep27 had enhanced antitumor activity against human renal cancer cells compared to the other groups. Further, hep27 mediated p53 and cleaved-PARP upregulation and mdm2 downregulation was involved and caused increased apoptosis. Moreover, F5/35-ZD55-Hep27 significantly suppressed tumor growth in subcutaneous renal cancer cell xenograft models. Our data demonstrated that 5/35 fiber-modified adenovirus F5/35-ZD55-Hep27 transferred into renal cancers efficiently and increased p53 to induce cancer cell apoptosis. Thus 5/35 fiber-modified adenoviral vector F5/35-ZD55-Hep27 might a promising vector and antitumor reagent for renal cancer gene therapy.

  10. Early evaluation of coastal nutrient over-enrichment: new procedures and indicators.

    Science.gov (United States)

    Goberville, Eric; Beaugrand, Grégory; Sautour, Benoit; Tréguer, Paul

    2011-08-01

    Recent studies have provided compelling evidence for an accelerated anthropogenic impact on coastal systems, resulting in intense inputs of materials and nutrients from the continent. This has led scientists and policymakers to encourage the implementation of monitoring programmes, which have resulted in the multiplicity of datasets. However surprisingly, only a few attempts have been made to couple observations with statistical and mathematical tools to detect, as soon as the data become available perturbations in coastal systems. Here, we propose new mathematical procedures to evaluate the state of a system, based on the building of relative reference state and indicators of nutrient over-enrichment. The techniques were tested in some French coastal systems using data from the programme SOMLIT. Applied to this dataset, the multivariate procedures rapidly identified and evaluated anthropogenic nutrient anomalies from the continent on three sites (Wimereux, Roscoff and Villefranche-sur-Mer) from 1997 onwards. Copyright © 2011 Elsevier Ltd. All rights reserved.

  11. Aggregative adherent strains of Corynebacterium pseudodiphtheriticum enter and survive within HEp-2 epithelial cells

    Directory of Open Access Journals (Sweden)

    Monica Cristina de Souza

    2012-06-01

    Full Text Available Corynebacterium pseudodiphtheriticum is a well-known human pathogen that mainly causes respiratory disease and is associated with high mortality in compromised hosts. Little is known about the virulence factors and pathogenesis of C. pseudodiphtheriticum. In this study, cultured human epithelial (HEp-2 cells were used to analyse the adherence pattern, internalisation and intracellular survival of the ATCC 10700 type strain and two additional clinical isolates. These microorganisms exhibited an aggregative adherence-like pattern to HEp-2 cells characterised by clumps of bacteria with a "stacked-brick" appearance. The differences in the ability of these microorganisms to invade and survive within HEp-2 cells and replicate in the extracellular environment up to 24 h post infection were evaluated. The fluorescent actin staining test demonstrated that actin polymerisation is involved in the internalisation of the C. pseudodiphtheriticum strains. The depolymerisation of microfilaments by cytochalasin E significantly reduced the internalisation of C. pseudodiphtheriticum by HEp-2 cells. Bacterial internalisation and cytoskeletal rearrangement seemed to be partially triggered by the activation of tyrosine kinase activity. Although C. pseudodiphtheriticum strains did not demonstrate an ability to replicate intracellularly, HEp-2 cells were unable to fully clear the pathogen within 24 h. These characteristics may explain how some C. pseudodiphtheriticum strains cause severe infection in human patients.

  12. Clinical and Radiographic Evaluation of Procedural Accidents and Errors during Root Canal Therapy

    OpenAIRE

    Mozayeni, Mohammad Ali; Asnaashari, Mohammad; Modaresi, Seyed Jalil

    2006-01-01

    INTRODUCTION: Root canal therapy (RCT)_like other dental practices_ can be accompanied with some accidents or unpredictable conditions that are called "procedural accidents". Having the knowledge about these accidents and their etiology is essential to have RCT completion and to prevent the repeat of these accidents. This study was designed to evaluate accidents occurring during RCT in patients referred to endodontic department of Shaheed Beheshti dental school during 2002. MATERIALS AND METH...

  13. Evaluation of intranasal Midazolam spray as a sedative in pediatric patients for radiological imaging procedures

    OpenAIRE

    Chokshi, Anisha A.; Patel, Vipul R; Chauhan, Parthiv R.; Patel, Deep J.; Indu A Chadha; Ramani, Monal N.

    2013-01-01

    Context: Preoperative anxiety and uncooperativeness experienced by pediatric patients are commonly associated with postoperative behavioral problems. Aims: We aimed to evaluate the efficacy and safety of intranasal Midazolam as a sedative in a pediatric age group for radiological imaging procedures and to note onset of sedation, level of sedation, condition of patient during separation from parents and effect on the cardio-respiratory system. Settings and Design: Randomized double-blinded stu...

  14. Evaluation of operation and death statistics at surgical procedures by using attribute control charts

    OpenAIRE

    Tomak, Leman; Bek, Yülsel

    2018-01-01

    Statistical Process Control (SPC) is a technique that enables the quality controllerto monitor, analyze, predict, control, and improve a production processthrough control charts. So to use of SPC in medical field is very important.The cases were obtained at the Ondokuz Mayis University Faculty of medicinedepartments of surgical sciences within two year were evaluated. The outcomesof the operations in these departments were followed throughout twenty-fourmonths. For a surgical procedure has tw...

  15. CLINICAL EVALUATION OF EFFECT OF EARLY EXPOSURE OF WOUNDS AFTER MINOR SURGICAL PROCEDURES

    OpenAIRE

    Annand Bansal; Rajesh Godara; Talo Millo; Rathindra Tripura; Jai Karan

    2015-01-01

    Application of dressings to surgical wounds is a custom as old as history and has become a ritual marking the completion of surgery. Under certain circumstances a surgical dressing might in fact predispose to the development of wound infection. This study evaluates clinical outcome of clean minor surgical wounds without use of surgical dressing. Material and methods: 100 consecutive patients presenting on an outpatient basis for minor soft tissue surgical procedures were studied with respect ...

  16. A new method to evaluate volumetric changes in sinus augmentation procedure.

    Science.gov (United States)

    Dellavia, Claudia; Speroni, Stefano; Pellegrini, Gaia; Gatto, Alessandra; Maiorana, Carlo

    2014-10-01

    In sinus augmentation procedure, the assessment of volume changes of grafted materials is important both in the clinical practice and in dental research to evaluate the features of filling materials. In this study, we assessed the repeatability of a new method proposed to evaluate volumetric changes following sinus lift augmentation procedure. In 10 patients, maxillary sinus augmentation procedure with simultaneous implant placement was performed. Maxillary cone beam computer tomographies were taken 1 week after surgery (T1) and 6 months after surgery (T2). At each evaluation the gap inside the implant between the fixture and the bottom of the screw was used as reference point (Rp), and a standardized volume of interest (VOI) centered on the Rp was selected. Masks were chosen to select the graft and bone tissue within the VOI; the volume at T1, T2, and the difference of volume between T1 and T2 were computed. Expert and non-expert operators performed the analysis. Method errors were computed. The error of the method was 1% for both intra-operator and inter-operator measurements. Tissue contraction at T2 was 19 ± 4% of the total initial volume. The standardization of the method allows to obtain repeatable measurements. © 2013 Wiley Periodicals, Inc.

  17. Hardware Testing and System Evaluation: Procedures to Evaluate Commodity Hardware for Production Clusters

    Energy Technology Data Exchange (ETDEWEB)

    Goebel, J

    2004-02-27

    Without stable hardware any program will fail. The frustration and expense of supporting bad hardware can drain an organization, delay progress, and frustrate everyone involved. At Stanford Linear Accelerator Center (SLAC), we have created a testing method that helps our group, SLAC Computer Services (SCS), weed out potentially bad hardware and purchase the best hardware at the best possible cost. Commodity hardware changes often, so new evaluations happen periodically each time we purchase systems and minor re-evaluations happen for revised systems for our clusters, about twice a year. This general framework helps SCS perform correct, efficient evaluations. This article outlines SCS's computer testing methods and our system acceptance criteria. We expanded the basic ideas to other evaluations such as storage, and we think the methods outlined in this article has helped us choose hardware that is much more stable and supportable than our previous purchases. We have found that commodity hardware ranges in quality, so systematic method and tools for hardware evaluation were necessary. This article is based on one instance of a hardware purchase, but the guidelines apply to the general problem of purchasing commodity computer systems for production computational work.

  18. Evaluation of N-Reactor piping decontamination procedure for effectiveness and materials corrosion

    Energy Technology Data Exchange (ETDEWEB)

    Kratzer, W.K.

    1967-08-01

    The effectiveness and corrosion characteristics of Turco 4512-A, an inhibited phosphoric acid, are being evaluated for N-Reactor primary system carbon steel decontamination by in-reactor decontamination tests, full scale component evaluations, and laboratory corrosion tests. To date these tests have showed that the decontamination procedure is effective for carbon steel decontamination and safe for all primary system materials that will be contacted by the solution. A few materials, namely, 17-4 pH SS, 416 SS, 420 SS, and Easy-Flo 45 braze alloy have not yet been completely tested. Results of their performance will be reported prior to full reactor decontamination.

  19. A Comparative Evaluation for Biologic Width following Surgical Crown Lengthening Using Gingivectomy and Ostectomy Procedure

    Directory of Open Access Journals (Sweden)

    Kiran Kumar Ganji

    2012-01-01

    Full Text Available Surgical crown lengthening has been proposed as a means of facilitating restorative procedures and preventing injuries in teeth with structurally inadequate clinical crown or exposing tooth structure in the presence of deep, subgingival pathologies which may hamper the access for proper restorative measures. Histological studies utilizing animal models have shown that postoperative crestal resorption allowed reestablishment of the biologic width. However, very little has been done in humans. Aims. The purpose of the study was to evaluate the potential changes in the periodontal tissues, particularly the biologic width, following surgical crown lengthening by two surgical procedures before and after crown placement. Methods and Material. Twenty (20 patients who needed surgical crown lengthening to gain retention necessary for prosthetic treatment and/or to access caries, tooth fracture, or previous prosthetic margins entered the study. The following parameters were obtained from line angles of treated teeth (teeth requiring surgical crown lengthening and adjacent sites: Plaque and Gingival Indices (PI & (GI, Position of Gingival Margin from reference Stent (PGMRS, Probing depth (PD, and Biologic Width (BW. Statistical Analysis Used. Student “t” Test. Results. Initial baseline values of biologic width were 2.55 mm (Gingivectomy procedure B1 Group and 1.95 mm (Ostectomy procedure B2 Group and after surgical procedure the values were 1.15 mm and 1.25 mm. Conclusions. Within the limitations of the study the biologic width, at treated sites, was re-established to its original vertical dimension by 3 months. Ostectomy with apically positioned flap can be considered as a more effective procedure than Gingivectomy for Surgical Crown Lengthening.

  20. Evaluation of treatment outcomes and clinical indications for antibiotic prophylaxis in patients undergoing implantation procedures.

    Science.gov (United States)

    Krasny, Marta; Krasny, Kornel; Zadurska, Małgorzata; Fiedor, Piotr

    2016-03-01

    The use of antibiotic therapy during implantation to reduce the risk of an early implant failure is widely discussed among clinicists. However, half an hour after the procedure a quarter of patients show bacteremia which could decrease the efficacy of the surgery. Implant failure is associated with destruction of bone tissue within the alveolar process and may lead to an alternative but compromised treatment plan. The aim of the study was to evaluate the influence of perioperative antibiotic protection on success of implantation. The retrospective study involved 1915 patients (females: 57.3%, males: 42.7%) with no systemic or local diseases, who required antibiotic therapy during surgical procedures. Group 1 comprised 203 patients with diagnosed vertical or horizontal bone atrophy within the alveolar ridge requiring reconstruction procedure before implantation. Group 2 included 1712 patients who did not need any surgical procedures before implantation. All the subjects took three types of antibiotics twice a day for 7 days. The data were statistically analyzed. A total number of 3309 implants were placed. Implantation efficacy in group 1 amounted to 98.53% and in group 2 it was 99.24%. Complications occurred most commonly after administration of cephalosporin which proved to be statistically significant for the patients who underwent augmentation with a bone block before the implant procedure (p 0.0209). Perioperative use of antibiotic therapy beneficially influences tissue healing, provides safety and success of the surgical procedure, as well as translates into high efficacy of implantation (99.52%). Copyright © 2015 Medical University of Bialystok. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

  1. The Application of Six Sigma Techniques in the Evaluation of Enzyme Measurement Procedures in China.

    Science.gov (United States)

    Zhang, Chuanbao; Zhao, Haijian; Wang, Jing; Zeng, Jie; Wang, Zhiguo

    2015-01-01

    Recently, Six Sigma techniques have been adopted by clinical laboratories to evaluate laboratory performance. Measurement procedures in laboratories can be categorized as "excellent", "good", and "improvement needed" based on sigma (σ) metrics of σ ≥ 6, 3 ≤ σ 1.2 indicates that the trueness of the procedure needs to be improved; 0.8 ≤ QGI ≤ 1.2 indicates that both the precision and trueness of the procedure need to be improved. Fresh frozen sera containing seven enzymes (ALT, ALP, AMY, AST, CK, GGT, and LDH) were sent to 78 clinical laboratories in China. The biases for measurement procedures in each laboratory (Bias) were calculated based on the target values assigned by 18 laboratories performing IFCC (International Federation of Clinical Chemistry and Laboratory medicine) recommended reference methods. The imprecision of each measurement procedure was represented by coefficient variations (CV) calculated based on internal quality control (IQC) data. The σ and QGI values were calculated as follows: σ = (TEa-Bias)/CV; QGI = Bias/(1.5 x CV). TEa is allowable total error for each enzyme derived from biological variation. Our study indicated that 7.9% (6/76, ALP) to 31.0% (18/58, AMY) of the participating laboratories were scored as "excellent" (σ ≥ 6), 21.1% (16/76, ALP) to 41.3% (31/75, CK) of the laboratories were scored as "good" (3 ≤ σ Six Sigma techniques still suggested that approximately 31.1% to 71.0% of the laboratories need to improve their enzyme measurement procedures, either in terms of precision, trueness, or both.

  2. CT-guided procedures: evaluation of a phantom system to teach accurate needle placement

    Energy Technology Data Exchange (ETDEWEB)

    Dimmick, S. [Department of Radiology, Princess Alexandra Hospital, Woolloongabba Queensland (Australia); Jones, M. [Queensland Clinical Trials Centre School of Population Health (University of Queensland), Princess Alexandra Hospital, Woolloongabba Queensland (Australia); Challen, J. [Department of Radiology, Princess Alexandra Hospital, Woolloongabba Queensland (Australia); Iedema, J. [Department of Radiology, Princess Alexandra Hospital, Woolloongabba Queensland (Australia); Wattuhewa, U. [Department of Radiology, Princess Alexandra Hospital, Woolloongabba Queensland (Australia); Coucher, J. [Department of Radiology, Princess Alexandra Hospital, Woolloongabba Queensland (Australia)]. E-mail: john_coucher@health.qld.gov.au

    2007-02-15

    Aim: To evaluate the use of a phantom system to help teach the basic techniques of accurate CT-guided needle placement, thereby avoiding the risks associated with teaching on patients. Materials and Methods: Gelatine phantoms with five, 1.9 cm embedded spherical wooden targets were constructed. Four trainee operators performed 15 simulated biopsy procedures on the targets (series one) and repeated identical procedures 2 weeks later (series two). Statistical analysis of accuracy of needle placement and subject confidence were performed. Results: Significant sequential improvement in axial plane angular error was noted with the average error decreasing by 0.33 degrees after every five procedures performed (95% CI: -0.58 to -0.08, p = 0.01). Operator confidence indicated significant improvement both within each series and from series one to series two (95% CI: 0.08 to 1.17, p = 0.025 and 95% CI: 0.00 to 0.58, p = 0.05) respectively. However, variability in operator performance made statistically significant improvement in other variables unproven. Conclusion: Despite the study comprising a relatively small number of participants and procedures, it clearly demonstrated the effectiveness of teaching operators to perform CT-guided procedures using a phantom system. Needle placement accuracy significantly improved, with a reduction in axial angular error, and improved operator confidence without the risks associated with training on patients. Three of the operators in this study had never performed a CT-guided procedure previously, and their proficiency, after a relatively short but intense period of training, was impressive. The use of phantoms should be considered routinely for basic training of CT-guided needle placement.

  3. Metabolism and cytotoxic effects of phosphatidylcholine hydroperoxide in human hepatoma HepG2 cells.

    Science.gov (United States)

    Suzuki, Yuuri; Nakagawa, Kiyotaka; Kato, Shunji; Tatewaki, Naoto; Mizuochi, Shunsuke; Ito, Junya; Eitsuka, Takahiro; Nishida, Hiroshi; Miyazawa, Teruo

    2015-03-20

    In this study, we investigated cellular uptake and metabolism of phosphatidylcholine hydroperoxide (PCOOH) in human hepatoma HepG2 cells by high performance liquid chromatography-tandem mass spectrometry, and then evaluated whether PCOOH or its metabolites cause pathophysiological effects such as cytotoxicity and apoptosis. Although we found that most PCOOH was reduced to PC hydroxide in HepG2 cells, the remaining PCOOH caused cytotoxic effects that may be mediated through an unusual apoptosis pathway. These results will enhance our fundamental understanding of how PCOOH, which is present in oxidized low density lipoproteins, is involved in the development of atherosclerosis. Copyright © 2015 Elsevier Inc. All rights reserved.

  4. Human factors evaluation of teletherapy: Human-system interfaces and procedures. Volume 3

    Energy Technology Data Exchange (ETDEWEB)

    Kaye, R.D.; Henriksen, K.; Jones, R. [Hughes Training, Inc., Falls Church, VA (United States); Morisseau, D.S.; Serig, D.I. [Nuclear Regulatory Commission, Washington, DC (United States). Div. of Systems Technology

    1995-07-01

    A series of human factors evaluations was undertaken to better understand the contributing factors to human error in the teletherapy environment. Teletherapy is a multidisciplinary methodology for treating cancerous tissue through selective exposure to an external beam of ionizing radiation. The principal sources of radiation are a radioactive isotope, typically cobalt60 (Co-60), or a linear accelerator device capable of producing very high energy x-ray and electron beams. A team of human factors specialists conducted site visits to radiation oncology departments at community hospitals, university centers, and free-standing clinics. In addition, a panel of radiation oncologists, medical physicists, and radiation technologists served as subject matter experts. A function and task analysis was initially performed to guide subsequent evaluations in the areas of user-system interfaces, procedures, training and qualifications, and organizational policies and practices. The present report focuses on an evaluation of the human-system interfaces in relation to the treatment machines and supporting equipment (e.g., simulators, treatment planning computers, control consoles, patient charts) found in the teletherapy environment. The report also evaluates operating, maintenance and emergency procedures and practices involved in teletherapy. The evaluations are based on the function and task analysis and established human engineering guidelines, where applicable.

  5. 45 CFR 303.109 - Procedures for State monitoring, evaluation and reporting on programs funded by Grants to States...

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Procedures for State monitoring, evaluation and... STANDARDS FOR PROGRAM OPERATIONS § 303.109 Procedures for State monitoring, evaluation and reporting on programs funded by Grants to States for Access and Visitation Programs. (a) Monitoring. The State must...

  6. Evaluation of cost and length of stay, linked to complications associated with major surgical procedures.

    Science.gov (United States)

    Pirson, M; Dehanne, F; Van den Bulcke, J; Leclercq, P; Martins, D; De Wever, A

    2017-06-19

    A lot of studies have demonstrated the possibility of reducing the number of post-operative complications in the domain of major surgical procedures with the use of medical preventive techniques. However, complications following surgical procedures are unfortunately frequent and are a major problem, not only because of the impact for the patient, but also because of economic consequences that they provoke. The aim of the present study is to evaluate the extra length of stay and the extra cost, born by the hospital and the social security, linked to complications, incurring after major surgical procedures. Study based on the data from 13 Belgian hospitals for the year 2012. Complications were extracted through medical discharge summaries. The cost born by the social security was assessed on the basis of the billing data, hospital cost are taken from cost accounting studies. The rate of complication for all the hospitals is 6.6%. About 30.3% of inpatient stays having a major or extreme severity of index had a complication during the stay, 1.8% of stays with a minor or moderate severity of index had a complication. The extra length of stay is 19.38 days when the stay has had a complication (p field of major surgical procedures having caused complications. Results should encourage Belgian authorities to propose and finance preventive measures in order to reduce these complications, which represent major economic impacts, not only for authorities but also for hospitals.

  7. Evaluation of intranasal Midazolam spray as a sedative in pediatric patients for radiological imaging procedures.

    Science.gov (United States)

    Chokshi, Anisha A; Patel, Vipul R; Chauhan, Parthiv R; Patel, Deep J; Chadha, Indu A; Ramani, Monal N

    2013-01-01

    Preoperative anxiety and uncooperativeness experienced by pediatric patients are commonly associated with postoperative behavioral problems. We aimed to evaluate the efficacy and safety of intranasal Midazolam as a sedative in a pediatric age group for radiological imaging procedures and to note onset of sedation, level of sedation, condition of patient during separation from parents and effect on the cardio-respiratory system. Randomized double-blinded study. Fifty patients of the pediatric age group of American Society of Anesthesiologist grade 2 and 3 who came for any radiological imaging procedures were studied. Patients were randomly allocated to receive, intranasally, either Midazolam 0.5 mg/kg (group A N = 25) or normal saline (group B N = 25) in both nostrils (0.25 mg/kg in each) 15 min before the procedure. Time for onset of sedation and satisfactory sedation, sedation score, separation score, hemodynamic changes and side-effects were recorded. Student's t-test. Intranasal Midazolam group had a significantly shorter time for onset of sedation and satisfactory sedation. Mean sedation score and mean separation score at 10 min and 15 min intervals were significant in intranasal Midazolam as compared with normal saline (P Intranasal Midazolam 0.5 mg/kg is safe and effective and provides adequate sedation for easy separation from the parents and reduced requirement of intravenous supplementation during radiological imaging procedures without any untoward side-effects.

  8. Evaluating Active Parental Consent Procedures for School Programming: Addressing the Sensitive Topic of Suicide Prevention.

    Science.gov (United States)

    Totura, Christine M Wienke; Kutash, Krista; Labouliere, Christa D; Karver, Marc S

    2017-02-01

    Suicide is the second leading cause of death for adolescents. Whereas school-based prevention programs are effective, obtaining active consent for youth participation in public health programming concerning sensitive topics is challenging. We explored several active consent procedures for improving participation rates. Five active consent methods (in-person, students taking forms home, mailing, mailing preceded by primers, mailing followed by reminder calls) were compared against passive consent procedures to evaluate recruitment success, as determined by participation (proportion who responded yes) and response (proportion who returned any response) rates. Participation acceptance rates ranged from 38 to 100% depending on consent method implemented. Compared with passive consent, active consent procedures were more variable in response and participation rates. In-person methods provided higher rates than less interpersonal methods, such as mailing or students taking consents home. Mailed primers before or reminder calls after consent forms were mailed increased response but not participation rates. Students taking consents home resulted in the lowest rates. Although passive consent produces the highest student participation, these methods are not always appropriate for programs addressing sensitive topics in schools. In-person active consent procedures may be the best option when prioritizing balance between parental awareness and successful student recruitment. © 2017, American School Health Association.

  9. Evaluation of Surface Cleaning Procedures for CTGS Substrates for SAW Technology with XPS

    Directory of Open Access Journals (Sweden)

    Erik Brachmann

    2017-11-01

    Full Text Available A highly efficient and reproducible cleaning procedure of piezoelectric substrates is essential in surface acoustic waves (SAW technology to fabricate high-quality SAW devices, especially for new applications such SAW sensors wherein new materials for piezoelectric substrates and interdigital transducers are used. Therefore, the development and critical evaluation of cleaning procedures for each material system that is under consideration becomes crucial. Contaminants like particles or the presence of organic/inorganic material on the substrate can dramatically influence and alter the properties of the thin film substrate composite, such as wettability, film adhesion, film texture, and so on. In this article, focus is given to different cleaning processes like SC-1 and SC-2, UV-ozone treatment, as well as cleaning by first-contact polymer Opticlean, which are applied for removal of contaminants from the piezoelectric substrate Ca 3 TaGa 3 Si 2 O 14 . By means of X-ray photoelectron spectroscopy, the presence of the most critical contaminants such as carbon, sodium, and iron removed through different cleaning procedures were studied and significant differences were observed between the outcomes of these procedures. Based on these results, a two-step cleaning process, combining SC-1 at a reduced temperature at 30 ∘ C instead of 80 ∘ C and a subsequent UV-ozone cleaning directly prior to deposition of the metallization, is suggested to achieve the lowest residual contamination level.

  10. Towards an HTTP Ecosystem for HEP Data Access

    Science.gov (United States)

    Furano, Fabrizio; Devresse, Adrien; Keeble, Oliver; Hellmich, Martin; Álvarez Ayllón, Alejandro

    2014-06-01

    In this contribution we present a vision for the use of the HTTP protocol for data access and data management in the context of HEP. The evolution of the DPM/LFC software stacks towards a modern framework that can be plugged into Apache servers triggered various initiatives that successfully demonstrated the use of HTTP-based protocols for data access, federation and transfer. This includes the evolution of the FTS3 system towards being able to manage third-party transfers using HTTP. Given the flexibility of the methods, the feature set may also include a subset of the SRM functionality that is relevant to disk systems. The application domain for such an ecosystem of services goes from large scale, Gridlike computing to the data access from laptops, profiting from tools that are shared with the Web community, like browsers, clients libraries and others. Particular focus was put into emphasizing the flexibility of the frameworks, which can interface with a very broad range of components, data stores, catalogues and metadata stores, including the possibility of building high performance dynamic federations of endpoints that build on the fly the feeling of a unique, seamless very efficient system. The overall goal is to leverage standards and standard practices, and use them to provide the higher level functionalities that are needed to fulfil the complex problem of Data Access in HEP. Other points of interest are about harmonizing the possibilities given by the HTTP/WebDAV protocols with existing frameworks like ROOT and already existing Storage Federations based on the XROOTD framework. We also provide quantitative evaluations of the performance that is achievable using HTTP for remote transfer and remote I/O in the context of HEP data. The idea is to contribute the parts that can make possible an ecosystem of services and applications, where the HEP-related features are covered, and the door is open to standard solutions and tools provided by third parties, in the

  11. HEP Science Network Requirements--Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Bakken, Jon; Barczyk, Artur; Blatecky, Alan; Boehnlein, Amber; Carlson, Rich; Chekanov, Sergei; Cotter, Steve; Cottrell, Les; Crawford, Glen; Crawford, Matt; Dart, Eli; Dattoria, Vince; Ernst, Michael; Fisk, Ian; Gardner, Rob; Johnston, Bill; Kent, Steve; Lammel, Stephan; Loken, Stewart; Metzger, Joe; Mount, Richard; Ndousse-Fetter, Thomas; Newman, Harvey; Schopf, Jennifer; Sekine, Yukiko; Stone, Alan; Tierney, Brian; Tull, Craig; Zurawski, Jason

    2010-04-27

    The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the US Department of Energy Office of Science, the single largest supporter of basic research in the physical sciences in the United States. In support of the Office of Science programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet to be a highly successful enabler of scientific discovery for over 20 years. In August 2009 ESnet and the Office of High Energy Physics (HEP), of the DOE Office of Science, organized a workshop to characterize the networking requirements of the programs funded by HEP. The International HEP community has been a leader in data intensive science from the beginning. HEP data sets have historically been the largest of all scientific data sets, and the communty of interest the most distributed. The HEP community was also the first to embrace Grid technologies. The requirements identified at the workshop are summarized below, and described in more detail in the case studies and the Findings section: (1) There will be more LHC Tier-3 sites than orginally thought, and likely more Tier-2 to Tier-2 traffic than was envisioned. It it not yet known what the impact of this will be on ESnet, but we will need to keep an eye on this traffic. (2) The LHC Tier-1 sites (BNL and FNAL) predict the need for 40-50 Gbps of data movement capacity in 2-5 years, and 100-200 Gbps in 5-10 years for HEP program related traffic. Other key HEP sites include LHC Tier-2 and Tier-3 sites, many of which are located at universities. To support the LHC, ESnet must continue its collaborations with university and international networks. (3) While in all cases the deployed 'raw' network bandwidth must exceed the user requirements in order to meet the data transfer and reliability requirements, network engineering for trans

  12. Nuclear medicine procedures for the evaluation of male sexual organs: a brief review

    Energy Technology Data Exchange (ETDEWEB)

    Bernardo-Filho, Mario; Santos-Filho, Sebastiao David; Fonseca, Adenilson de Souza da [Universidade do Estado do Rio de Janeiro (UERJ), RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes. Lab. de Radiofarmacia Experimental; Carter, Kevin [Harvard Univ., Boston, MA (United States). Brigham and Women' s Hospital. Dept. of Radiology; Missailidis, Sotiris [The Open University, Milton Keynes (United Kingdom). Dept. of Chemistry and Analytical Sciences

    2008-12-15

    Sexuality consists of three aspects that are interrelated and inseparable, biological, physiological and social. The biological aspect considers the individual's capability to give and to receive pleasure. In consequence, it covers the functionality of the sexual organs and the physiology of human sexual response cycle. Diagnostic imaging modalities, such as single photon emission computed tomography (SPECT) and positron emission tomography (PET) have been used to evaluate clinical disorders of the male reproductive system. PET and SPECT procedures basically involve the administration of a radiopharmaceutical that has a higher uptake in a specific tumor or tissue. The aim of this brief review is to present some radiopharmaceuticals that have been used in the clinical evaluation of the male sexual organs (testes, prostate, seminal vesicles, penis) related with male sexuality. This information could be useful in better understanding the male sexual response cycle, as well as the sexual disorders, when considering the male sexual organs and the pelvic floor. Moreover, the findings obtained with PET and SPECT imaging could help to evaluate the efficacy of clinical results of therapeutic procedures. In conclusion, the knowledge from these images could aid in better understanding the physiology of the different organs related with sexuality. Furthermore, they could be important tools to evaluate the physiological integrity of the involved organs, to improve clinical strategies and to accompany the patients under treatment. (author)

  13. Modelling hadronic interactions in HEP MC generators

    CERN Document Server

    Skands, Peter

    2015-01-01

    HEP event generators aim to describe high-energy collisions in full exclusive detail. They combine perturbative matrix elements and parton showers with dynamical models of less well-understood phenomena such as hadronization, diffraction, and the so-called underlying event. We briefly summarise some of the main concepts relevant to the modelling of soft/inclusive hadron interactions in MC generators, in particular PYTHIA, with emphasis on questions recently highlighted by LHC data.

  14. Evaluation of cytotoxic effect of methanolic extracts isolated from endemic plants of Chaharmahal va Bakhtiari province on PC-3, MCF-7, Hep G2, CHO and B16-F10 cell lines

    Directory of Open Access Journals (Sweden)

    Z. Tayarani-Najaran

    2017-11-01

    Full Text Available Background and objectives: To date, thousands of secondary metabolites have been isolated from plants and microorganisms and there is an unprecedented attention towards potential biomedical applications of natural compounds. In this study, cytotoxic properties of methanol extracts of Stachys obtusicrena, Aristolochia olivieri, Linum album, Dionysia sawyeri, Ajuga chamaecistus, Achillea kellalensis, Nepeta glomerulosa, Phlomis aucheria, Tanacetum dumosum, Dianthus orientalis, Scutellaria multicaulis, Cicer oxyodon and Picris oligocephalum which are widely grown in Iran, were investigated on PC-3 (prostat cancer, MCF-7 (breast cancer, Hep-G2 (liver cancer, CHO (ovarian cancer and B16-F10 (melanoma cell lines. Methods: The cancer cells were cultured in RPMI-1640 and incubated with different concentrations of the plant extracts. Cell viability was quantitated by Alamar blue® assay. The apoptotic cells were determined by PI coloring and Flow Cytometry (Sub-G1 peak. Results: The methanol extracts of D. sawyeri, S. obtusicrena, and C. oxyodon significantly decreased the viability of CHO cells. The Methanol extract of D. sawyer and L. album had cytotoxic effects on B16-F10 cells, whereas no toxicity was observed in MCF-7, Hep-G2 and PC-3 cell lines after incubation of the cancer cells with the plant extracts. The PI staining results showed that D. sawyeri, S. obtusicrena, and C. oxyodon in CHO cancer cells could induce apoptosis in a concentration-dependent manner. Conclusion: Screening plants to find the most cytotoxic extract showed D. sawyeri, S. obtusicrena, C. oxyodon and L. album had the potential for further analysis toward finding active phytochemicals with cytotoxic activity.

  15. Percutaneous needle biopsy of the renal allograft using the automated needle system: evaluation of 87 procedures.

    Science.gov (United States)

    Nahas, W C; Mazzucchi, E; Henrique, A; Ianhez, L E; Saldanha, L B; Sabbaga, E; Arap, S

    1993-08-01

    Between April 1991 and February 1992 we obtained 87 percutaneous core needle biopsy specimens from 60 patients with the automatic biopsy gun. In 78 cases (89.7%) enough renal tissue was obtained for histological analysis. Acute rejection was present on 35 biopsies (40.3%) indicating antirejection therapy. No pathological signs of rejection were detected on 43 biopsies (49.4%). In 38 instances (43.7%) biopsy diagnosis differed from clinical presumptive diagnosis, affecting patient management, while in the remainder it was helpful to confirm clinical impressions. The only complication of the procedure was gross hematuria (requiring vesical irrigation in 1 patient). Due to its simplicity and low morbidity rate, renal biopsy with the automated needle system is the procedure of choice for evaluation and management of renal allograft dysfunction.

  16. Future HEP Accelerators: The US Perspective

    Energy Technology Data Exchange (ETDEWEB)

    Bhat, Pushpalatha [Fermilab; Shiltsev, Vladimir [Fermilab

    2015-11-02

    Accelerator technology has advanced tremendously since the introduction of accelerators in the 1930s, and particle accelerators have become indispensable instruments in high energy physics (HEP) research to probe Nature at smaller and smaller distances. At present, accelerator facilities can be classified into Energy Frontier colliders that enable direct discoveries and studies of high mass scale particles and Intensity Frontier accelerators for exploration of extremely rare processes, usually at relatively low energies. The near term strategies of the global energy frontier particle physics community are centered on fully exploiting the physics potential of the Large Hadron Collider (LHC) at CERN through its high-luminosity upgrade (HL-LHC), while the intensity frontier HEP research is focused on studies of neutrinos at the MW-scale beam power accelerator facilities, such as Fermilab Main Injector with the planned PIP-II SRF linac project. A number of next generation accelerator facilities have been proposed and are currently under consideration for the medium- and long-term future programs of accelerator-based HEP research. In this paper, we briefly review the post-LHC energy frontier options, both for lepton and hadron colliders in various regions of the world, as well as possible future intensity frontier accelerator facilities.

  17. The Use of HepRep in GLAST

    Energy Technology Data Exchange (ETDEWEB)

    Perl, Joseph

    2003-07-10

    HepRep is a generic, hierarchical format for description of graphics representables that can be augmented by physics information and relational properties. It was developed for high energy physics event display applications and is especially suited to client/server or component frameworks. The GLAST experiment, an international effort led by NASA for a gamma-ray telescope to launch in 2006, chose HepRep to provide a flexible, extensible and maintainable framework for their event display without tying their users to any one graphics application. To support HepRep in their GUADI infrastructure, GLAST developed a HepRep filler and builder architecture. The architecture hides the details of XML and CORBA in a set of base and helper classes allowing physics experts to focus on what data they want to represent. GLAST has two GAUDI services: HepRepSvc, which registers HepRep fillers in a global registry and allows the HepRep to be exported to XML, and CorbaSvc, which allows the HepRep to be published through a CORBA interface and which allows the client application to feed commands back to GAUDI (such as start next event, or run some GAUDI algorithm). GLAST's HepRep solution gives users a choice of client applications, WIRED (written in Java) or FRED (written in C++ and Ruby), and leaves them free to move to any future HepRep-compliant event display.

  18. Técnica de biópsia hepática em eqüino por laparoscopia Hepatic biopsy in horses by laparoscopy

    Directory of Open Access Journals (Sweden)

    Luis Cláudio Lopes Correia da Silva

    2002-06-01

    Full Text Available Este trabalho experimental objetivou a avaliação do uso da laparoscopia para biópsia hepática em eqüino e o estudo das possíveis alterações clínicas e laboratoriais decorrentes de tal procedimento. Foram utilizados 10 animais, os quais foram submetidos a procedimento cirúrgico em posição quadrupedal, por abordagem pelo flanco direito, no 17º espaço intercostal, após jejum alimentar e hídrico de 36 e 18 horas respectivamente. A avaliação pós-operatória constou de exame físico diário e colheita de amostras de sangue seriadas. O procedimento possibilitou inspeção dos órgãos e estruturas anatômicas localizados dorsalmente à direita da cavidade abdominal, favorecendo a escolha do local de biópsia hepática. Não foram observadas alterações no exame físico dos animais. Os exames hematológicos e de função hepática não apresentaram alterações significativas. Concluiu-se que o emprego da laparoscopia para realização de biópsia hepática em eqüino é simples, eficaz e seguro.The purpose of this research was to evaluate the use of laparoscopy in obtaining hepatic biopsy in horses and to determine a possible clinical and laboratorial changes related to the procedure. Ten animals were submitted to 36 hours of fastening and 18 hours of water deprivation and then operated in the standing position, through a right flank approach at the level of the 17th intercostal space. Post-operative evaluation consisted of daily physical examination and seriated blood sampling. The procedure allowed for wide visualization of the viscera and anatomical structures located on the right side of the abdominal cavity, favouring the choice of the spot for hepatic biopsy collection. No changes were noted in the physical state of the animals and alterations in hemogram and hepatic function tests were not statistically significant. It was concluded that laparoscopy is a simple, effective and safe technique for obtaining hepatic biopsy

  19. Financial and testamentary capacity evaluations: procedures and assessment instruments underneath a functional approach.

    Science.gov (United States)

    Sousa, Liliana B; Simões, Mário R; Firmino, Horácio; Peisah, Carmelle

    2014-02-01

    Mental health professionals are frequently involved in mental capacity determinations. However, there is a lack of specific measures and well-defined procedures for these evaluations. The main purpose of this paper is to provide a review of financial and testamentary capacity evaluation procedures, including not only the traditional neuropsychological and functional assessment but also the more recently developed forensic assessment instruments (FAIs), which have been developed to provide a specialized answer to legal systems regarding civil competencies. Here the main guidelines, papers, and other references are reviewed in order to achieve a complete and comprehensive selection of instruments used in the assessment of financial and testamentary capacity. Although some specific measures for financial abilities have been developed recently, the same is not true for testamentary capacity. Here are presented several instruments or methodologies for assessing financial and testamentary capacity, including neuropsychological assessment, functional assessment scales, performance based functional assessment instruments, and specific FAIs. FAIs are the only specific instruments intended to provide a specific and direct answer to the assessment of financial capacity based on legal systems. Considering the need to move from a diagnostic to a functional approach in financial and testamentary capacity evaluations, it is essential to consider both general functional examination as well as cognitive functioning.

  20. Radiolabeled localization of the sentinel lymph node: dosimetric evaluation in personnel involved in the procedure.

    Science.gov (United States)

    Pelosi, E; Arena, V; Bellò, M; Cesana, P; Lamberti, L; Spandonari, T; Ropolo, R; Sandrucci, S; Bisi, G

    2002-01-01

    Peritumoral injection of 99mTc-labeled colloids for lymphoscintigraphy and radioguided surgery does not entail any relevant radiation burden to the patients. The real issue about radiation protection concerns the personnel involved in the procedure besides the nuclear medicine personnel. The aim of our study was to evaluate the cumulative doses to personnel involved during the injection of radiolabeled compounds, under ultrasound or stereotactic guidance and the radiation burden to the personnel involved in the surgical incision of the tumor 24 hours after the administration of 99mTc-labeled colloids. We performed environmental contamination tests (SMEAR TEST) and exposure evaluation in the operating room. In the operating room the removed activity in the analyzed samples was less than 0.5 Bq/g and exposure to the personnel was less than 6 micro Sv/h. The evaluations made during ultrasound guidance demonstrated an equivalent and effective dose less than 20 microSv. Our results show that during ultrasound or stereotactic administration of radiolabeled compounds the radiation burden to the personnel involved in the procedure is virtually negligible. The surgeons too are exposed to a negligible radiation dose.

  1. Using X-ray computed tomography to evaluate the initial saturation resulting from different saturation procedures

    DEFF Research Database (Denmark)

    Christensen, Britt Stenhøj Baun; Wildenschild, D; Jensen, K.H.

    2006-01-01

    for saturation. Evaluation of the different enhanced saturation techniques was done with Xray computed tomography (CT) and gravimetrically. The use of CT scanning makes it possible to observe the spatial distribution of wetting and non-wetting phases in the porous medium in a non-destructive way. In this case...... with pressurized nitrogen between each saturation and allowed to saturate for the same length of time for all the different procedures. Both gravimetric measurements and CT attenuation levels showed that venting the sample with carbon dioxide prior to saturation clearly improved initial saturation whereas the use...

  2. Evaluation of a New Automated Processing System (TACAS™ Pro) for Liquid-Based Procedures.

    Science.gov (United States)

    Kuramoto, Hiroyuki; Sugimoto, Naoko; Iwami, Yoshiko; Kato, Chizuyo; Hori, Masuko; Iida, Manichi

    2015-01-01

    To evaluate a fully automated processing system (TACAS™ Pro) for liquid-based procedures (LBPs). Materials were 3,483 and additionally 502 specimens that were taken at Kanagawa Health Service Association. Specimens obtained with a Cervex-Brush® were first smeared to glass slides using one side of the brush and then processed to TACAS Pro. (1) The microscopy watching time per normal case was 3.65 ± 0.85 min in the conventional procedure, whereas in the LBP it was 1.95 ± 0.60 min, and the latter reduced workload to 53%. (2) The handling time of TACAS Pro per day was 2 h and 25.8 min. The workload at a laboratory offset it and revealed the work saving to be 63.8%. (3) Unsatisfactory rates were 0% in the conventional procedure, whereas in the LBP it was 1.88% at first. The latter rate decreased to 0.5% after system improvement. (4) Specimens which may disturb microscopy analysis were found in 1.06%, including 3 cases of possible carry-over of cells to the following slides. An additional study with the revised system confirmed no carry-over. (5) Incidences of abnormal cytology were consistent between the two methods. The revised automated processing system TACAS Pro is a feasible and useful LBP and reduces the workload of cytology laboratories. © 2015 S. Karger AG, Basel.

  3. Is the subscapularis normal after the open Latarjet procedure? An isokinetic and magnetic resonance imaging evaluation.

    Science.gov (United States)

    Caubère, Alexandre; Lami, Damien; Boileau, Pascal; Parratte, Sébastien; Ollivier, Matthieu; Argenson, Jean-Noël

    2017-10-01

    The Latarjet procedure is considered to be a violation of the subscapularis muscle. This study evaluated the postoperative status of the subscapularis through isokinetic and magnetic resonance imaging analysis after splitting. We hypothesized that compared with a healthy contralateral shoulder, there would be satisfactory recovery of subscapularis strength at the cost of some fatigability and some mild fatty infiltration. This was a case-control retrospective study of patients who underwent a Latarjet procedure between January 2013 and January 2015. A total of 20 patients were reviewed at 1 year postoperatively. With the patient seated, strength testing of both shoulders was done (concentric, eccentric, and fatigability) with a dynamometer. Trophicity and fatty infiltration were analyzed by magnetic resonance imaging. Strength of the internal rotators (IRs) and external rotators (ERs) of the injured shoulder was significantly lower compared with the healthy shoulder in concentric testing at 180°/s and 60°/s (13% for IR and 20% for E, P Latarjet procedure, isokinetic testing showed a combined strength deficit in both internal and external rotation with a conserved muscle balance. Although no significant subscapularis fatty infiltration or atrophy was noted, there was a significant deficit in endurance compared with the healthy shoulder. Copyright © 2017 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Elsevier Inc. All rights reserved.

  4. A procedure to evaluate the efficiency of utilization of dietary amino acid for poultry

    Directory of Open Access Journals (Sweden)

    Edney Pereira da Silva

    2014-05-01

    Full Text Available The aim of this study was to develop a procedure based on Gompertz function to determine the efficiency of utilization of amino acid. The procedure was applied to determine the efficiency of utilization of dietary lysine, methionine+cystine and threonine by growing pullets and based on the efficiencies were estimated the requirements for the growth phase of birds. The Gompertz function was fitted to the data of feed intake, body weight, feather-free body protein weight and feather protein weight of four strains of laying hens in the growth phase. The rates of consumption and daily protein deposition (PD were calculated. The amino acid deposition was obtained by multiplying the PD by the amino acid concentration in feather protein and feather-free body protein. The results showed that the efficiency of utilization of amino acid decreased with maturity and, conversely, there was a proportional increase of the requirement per kg of weight gain. The procedure based on the Gompertz function to determine the efficiency of utilization of amino acid proved to be suitable to evaluate the efficiency of utilization of amino acid and can be a useful tool to diagnose the effectiveness of the nutritional management, aiding in decision-making on the nutritional management.

  5. Optimizing experimental procedures for quantitative evaluation of crop plant performance in high throughput phenotyping systems

    Directory of Open Access Journals (Sweden)

    Astrid eJunker

    2015-01-01

    Full Text Available Detailed and standardized protocols for plant cultivation in environmentally controlled conditions are an essential prerequisite to conduct reproducible experiments with precisely defined treatments. Setting up appropriate and well defined experimental procedures is thus crucial for the generation of solid evidence and is thus indispensable for successful plant research. Non-invasive and high throughput (HT phenotyping technologies offer the opportunity to monitor and quantify performance dynamics of several hundreds of plants at a time. Compared to small scale plant cultivations, HT systems have much higher demands, from a conceptual and a logistic point of view, on experimental design, as well as the actual plant cultivation conditions, and the image analysis and statistical methods for data evaluation. Furthermore, cultivation conditions need to be designed that elicit plant performance characteristics corresponding to those under natural conditions. This manuscript describes critical steps in the optimization of procedures for HT plant phenotyping systems. Starting with the model plant Arabidopsis, HT-compatible methods were tested, and optimized with regard to growth substrate, soil coverage, watering regime, experimental design (considering environmental inhomogeneities in automated plant cultivation and imaging systems. As revealed by metabolite profiling, plant movement did not affect the plants’ physiological status. Based on these results, procedures for maize HT cultivation and monitoring were established. Variation of maize vegetative growth in the HT phenotyping system did match well with that observed in the field. The presented results outline important issues to be considered in the design of HT phenotyping experiments for model and crop plants. It thereby provides guidelines for the setup of HT experimental procedures, which are required for the generation of reliable and reproducible data of phenotypic variation for a broad

  6. Optimizing experimental procedures for quantitative evaluation of crop plant performance in high throughput phenotyping systems.

    Science.gov (United States)

    Junker, Astrid; Muraya, Moses M; Weigelt-Fischer, Kathleen; Arana-Ceballos, Fernando; Klukas, Christian; Melchinger, Albrecht E; Meyer, Rhonda C; Riewe, David; Altmann, Thomas

    2014-01-01

    Detailed and standardized protocols for plant cultivation in environmentally controlled conditions are an essential prerequisite to conduct reproducible experiments with precisely defined treatments. Setting up appropriate and well defined experimental procedures is thus crucial for the generation of solid evidence and indispensable for successful plant research. Non-invasive and high throughput (HT) phenotyping technologies offer the opportunity to monitor and quantify performance dynamics of several hundreds of plants at a time. Compared to small scale plant cultivations, HT systems have much higher demands, from a conceptual and a logistic point of view, on experimental design, as well as the actual plant cultivation conditions, and the image analysis and statistical methods for data evaluation. Furthermore, cultivation conditions need to be designed that elicit plant performance characteristics corresponding to those under natural conditions. This manuscript describes critical steps in the optimization of procedures for HT plant phenotyping systems. Starting with the model plant Arabidopsis, HT-compatible methods were tested, and optimized with regard to growth substrate, soil coverage, watering regime, experimental design (considering environmental inhomogeneities) in automated plant cultivation and imaging systems. As revealed by metabolite profiling, plant movement did not affect the plants' physiological status. Based on these results, procedures for maize HT cultivation and monitoring were established. Variation of maize vegetative growth in the HT phenotyping system did match well with that observed in the field. The presented results outline important issues to be considered in the design of HT phenotyping experiments for model and crop plants. It thereby provides guidelines for the setup of HT experimental procedures, which are required for the generation of reliable and reproducible data of phenotypic variation for a broad range of applications.

  7. Use of Preclinical Drug vs. Food Choice Procedures to Evaluate Candidate Medications for Cocaine Addiction.

    Science.gov (United States)

    Banks, Matthew L; Hutsell, Blake A; Schwienteck, Kathryn L; Negus, S Stevens

    2015-06-01

    Drug addiction is a disease that manifests as an inappropriate allocation of behavior towards the procurement and use of the abused substance and away from other behaviors that produce more adaptive reinforcers (e.g. exercise, work, family and social relationships). The goal of treating drug addiction is not only to decrease drug-maintained behaviors, but also to promote a reallocation of behavior towards alternative, nondrug reinforcers. Experimental procedures that offer concurrent access to both a drug reinforcer and an alternative, nondrug reinforcer provide a research tool for assessment of medication effects on drug choice and behavioral allocation. Choice procedures are currently the standard in human laboratory research on medications development. Preclinical choice procedures have been utilized in biomedical research since the early 1940's, and during the last 10-15 years, their use for evaluation of medications to treat drug addiction has increased. We propose here that parallel use of choice procedures in preclinical and clinical studies will facilitate translational research on development of medications to treat cocaine addiction. In support of this proposition, a review of the literature suggests strong concordance between preclinical effectiveness of candidate medications to modify cocaine choice in nonhuman primates and rodents and clinical effectiveness of these medications to modify either cocaine choice in human laboratory studies or metrics of cocaine abuse in patients with cocaine use disorder. The strongest evidence for medication effectiveness in preclinical choice studies has been obtained with maintenance on the monoamine releaser d-amphetamine, a candidate agonist medication for cocaine use analogous to use of methadone to treat heroin abuse or nicotine formulations to treat tobacco dependence.

  8. An evaluation of the implicit management procedure used for some ICES roundfish stocks

    DEFF Research Database (Denmark)

    Kell, L.T.; Pilling, G.M.; Kirkwood, G.P.

    2005-01-01

    the types of projection used by ICES do not incorporate important lags between assessing stock status and implementing management measures, and they also ignore important sources of uncertainty about the actual dynamics, as well as our ability to collect data and implement management regulations (i.e. model...... the plausible range of characteristics of the underlying dynamics, but not necessarily model their full complexity. In general, they will be more complex than those used by assessment working groups, so developing management procedures that are robust to a broad range of uncertainty. However, the models......This paper describes a simulation study that evaluated the performance of the scientific advisory process used by ICES to recommend total allowable catches (TACs) for roundfish stocks. A "management strategy evaluation" approach is used, involving development of an operating model to represent...

  9. Environmental risk assessment for plant pests: a procedure to evaluate their impacts on ecosystem services

    DEFF Research Database (Denmark)

    Gilioli, G.; Schrader, G.; Baker, R.H.A.

    2014-01-01

    . By including both the structural and functional components of the environment threatened by invasive alien species (IAS), in particular plant pests, we propose an environmental risk assessment scheme that addresses this complexity. Structural components are investigated by evaluating the impacts of the plant...... Risk Analysis. The method enables the assessment of overall environmental risk which integrates the impacts on different components of the environment and their probabilities of occurrence. The application of the proposed scheme is illustrated by evaluating the environmental impacts of the invasive......The current methods to assess the environmental impacts of plant pests differ in their approaches and there is a lack of the standardized procedures necessary to provide accurate and consistent results, demonstrating the complexity of developing a commonly accepted scheme for this purpose...

  10. Does the dynamic sling effect of the Latarjet procedure improve shoulder stability? A biomechanical evaluation.

    Science.gov (United States)

    Giles, Joshua W; Boons, Harm W; Elkinson, Ilia; Faber, Kenneth J; Ferreira, Louis M; Johnson, James A; Athwal, George S

    2013-06-01

    Glenohumeral instability with glenoid bone loss is commonly treated with the Latarjet procedure. The procedure involves transfer of the coracoid and conjoint tendon, which is thought to provide a stabilizing sling effect; however, its significance is unknown. This study evaluated the effects of the Latarjet procedure, with and without conjoint tendon loading, on shoulder stability and range of motion (ROM). A custom simulator was used to evaluate anterior shoulder stability and ROM in 8 cadaveric shoulders. Testing conditions included intact, 30% glenoid defect, and Latarjet with and without conjoint loading. Unloaded and 10-N loaded states were tested in adduction and 90° abduction. Outcome variables included dislocation, stiffness (neutral and 60° external rotation), and internal-external rotational ROM. All 30% defects dislocated in abduction external rotation. The loaded Latarjet prevented dislocation in all specimens, whereas the unloaded Latarjet stabilized 6 of 8 specimens. In abduction external rotation, there were no significant differences in stiffness between loaded and unloaded transfers (P = .176). In adduction, there were no significant differences between the intact and the loaded Latarjet (P ≥ .228); however, in neutral rotation, the unloaded Latarjet (P = .015) and the 30% defects (P = .011) were significantly less stiff. Rotational ROM in abduction was significantly reduced with the loaded Latarjet (P = .014) compared with unloaded Latarjet, and no differences were found in adduction. These findings indicate that glenohumeral stability is improved, but not fully restored to intact, with conjoint tendon loading. The results support the existence of the sling effect and its importance in augmenting stability provided by the transferred coracoid. Copyright © 2013 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Mosby, Inc. All rights reserved.

  11. A prospective patient-focused evaluation of the tolerance and acceptability of a stereotactic radiosurgery procedure.

    Science.gov (United States)

    Williams, Janet R; Venchiarutti, Rebecca L; Smee, Robert

    2017-06-01

    Stereotactic radiosurgery (SRS) is a frequently used non-surgical procedure to treat benign and malignant brain lesions. Few studies have focused on patient perceptions of SRS. The aims of this patient-focused study were to assess patient experiences of SRS, and changes in patient-reported symptoms over 12weeks post-SRS. Using the 6-point Likert Scoring Scale in a diary-format for a less discriminatory evaluation, patients self-reported presence or absence, and severity of physical and psychological symptoms within 24h, 1-week, and 12-weeks post-SRS. Non-parametric repeated measures ANOVA was used to evaluate changes in symptoms. Of the 748 recruited patients, 690 returned the first diary (92%), while 564 patients returned all three diaries for matched responses analysis (82%). Three-quarters of 690 patients reported receiving clear verbal explanations and printed material prior to their procedure, and 99% reported the clinical team were 'very supportive' or gave 'wonderful care'. Fatigue (82%) and headaches (65%) were the most frequently reported symptoms within 24-h post-SRS. Over 12weeks, patients reported significant reductions in headache, nausea, fatigue, anxiety and tension (p<0.001); loss of balance and concentration significantly increased by 12-weeks post-SRS (p<0.001). Some patients attributed symptoms such as fatigue or headaches to the demands of the procedure day. Findings of this study reflect the need to further research patients' physical and psychological symptoms post-SRS, which may differ from the clinicians' perception of the effects of treatment. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Evaluation of Acid Digestion Procedures to Estimate Mineral Contents in Materials from Animal Trials

    Directory of Open Access Journals (Sweden)

    M. N. N. Palma

    2015-11-01

    Full Text Available Rigorously standardized laboratory protocols are essential for meaningful comparison of data from multiple sites. Considering that interactions of minerals with organic matrices may vary depending on the material nature, there could be peculiar demands for each material with respect to digestion procedure. Acid digestion procedures were evaluated using different nitric to perchloric acid ratios and one- or two-step digestion to estimate the concentration of calcium, phosphorus, magnesium, and zinc in samples of carcass, bone, excreta, concentrate, forage, and feces. Six procedures were evaluated: ratio of nitric to perchloric acid at 2:1, 3:1, and 4:1 v/v in a one- or two-step digestion. There were no direct or interaction effects (p>0.01 of nitric to perchloric acid ratio or number of digestion steps on magnesium and zinc contents. Calcium and phosphorus contents presented a significant (p0.01 calcium or phosphorus contents in carcass, excreta, concentrate, forage, and feces. Number of digestion steps did not affect mineral content (p>0.01. Estimated concentration of calcium, phosphorus, magnesium, and zinc in carcass, excreta, concentrated, forage, and feces samples can be performed using digestion solution of nitric to perchloric acid 4:1 v/v in a one-step digestion. However, samples of bones demand a stronger digestion solution to analyze the mineral contents, which is represented by an increased proportion of perchloric acid, being recommended a digestion solution of nitric to perchloric acid 2:1 v/v in a one-step digestion.

  13. Evaluation of five procedures for measuring nonstandard doses of nifedipine liquid.

    Science.gov (United States)

    Rosen, W J; Johnson, C E

    1989-11-01

    Five methods for removing the maximum volume of liquid from a nifedipine capsule were evaluated for efficiency and reproducibility, and the concentration of nifedipine in Procardia and Adalat 10- and 20-mg capsules was determined The methods evaluated were the needle puncture and squeeze method; the scissors method; the needle and syringe, one-hole method; the needle and syringe, two-hole method; and the capsule in syringe barrel method. The liquid content of 10 Adalat 10-mg capsules was removed by each method, the volume was determined by the weight and specific gravity of the nifedipine liquid, and the mean volumes of nifedipine liquid were compared. A stepwise procedure for using the needle and syringe, one-hole method was developed. The mean nifedipine concentration in Procardia 10- and 20-mg capsules was determined by ultraviolet spectrophotometry by using 30 capsules of each brand and strength. The needle and syringe methods were the most reproducible and efficient procedures for obtaining a desired milligram dosage of nifedipine from the capsules. The two-hole method would be less practical in the clinical setting, however, because it is time consuming and cumbersome. The mean assayed nifedipine concentration in the Procardia and Adalat 10- and 20-mg capsules was within 10% of the concentration reported by the manufacturer for each specific product lot number. Based on reproducibility and efficiency, the needle and syringe methods are the best methods for quantitation of liquid nifedipine doses for patient administration.

  14. Heel lance in newborn during breastfeeding: an evaluation of analgesic effect of this procedure

    Directory of Open Access Journals (Sweden)

    Tozzini Danila

    2008-11-01

    Full Text Available Abstract Objectives The reduction of pain due to routine invasive procedures (capillary heel stick blood sampling for neonatal metabolic screening in the newborn is an important objective for the so-called "Hospital with no pain". Practices such as skin to skin contact, or breastfeeding, in healthy newborn, may represent an alternative to the use of analgesic drugs. The aim of our work is to evaluate the analgesic effect of breastfeeding during heel puncture in full term healthy newborn. Methods We studied 200 healthy full term newborns (100 cases and 100 controls, proposing the puncture to mothers during breastfeeding, and explaining to them all the advantages of this practice. Pain assessment was evaluated by DAN scale (Douleur Aigue Nouveau ne scale. Results The difference in score of pain according to the DAN scale was significant in the two groups of patients (p = 0.000; the medium score was 5.15 for controls and 2.65 for cases (newborns sampled during breastfeeding. Conclusion Our results confirmed the evidence of analgesic effect of breastfeeding during heel puncture. This procedure could easily be adopted routinely in maternity wards.

  15. Procedure to evaluate the ionizing radiation influence over LED and magnetic induction lamps

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Otavio Luis de; Menzel, Silvio Carlos, E-mail: otavioluis@ipen.br, E-mail: scmenzel@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (CEN/IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Engenharia Nuclear; Ribas, Jacinto Oliveira, E-mail: jacinto@eletronuclear.gov.br [Eletrobras Termonuclear S.A. (ELETRONUCLEAR), Angra dos Reis, RJ (Brazil). Gerencia de Eletrica e Instrumentacao

    2015-07-01

    The goal of this paper is to present a methodology to evaluate the ionizing radiation influence over Lighting Emitting Diode (LED) and Magnetic Induction (MI) lamps as they use a lot of electronic in their power supply. Considering they have a huge lifetime it is interesting to apply this technology into environments under ionizing radiation, such as a nuclear facility. Thus, it is possible to increase the period between two consecutive maintenance, reduce the repair and global maintenance costs and reduce the operational personnel exposure to ionizing radiation. In this context it is going to be presented a scheme to select different LED and MI lamps available in the Brazilian market, a methodology to irradiate several lamp samples according various radiation levels that can be found in the facilities and the electrical and photometric evaluation to be performed. Considering this methodology it will be possible to analyze the lamps capacity to withstand ionizing radiation, under regular operating conditions of the facilities and its effects in the performance and lifetime of the selected lamps. Thus, the procedures suggested in this work can be used as a guide to perform experiments and analysis to find specific lamps that can reduce the global maintenance costs and the personnel exposure. Hereafter, several lamps are going to be acquired and the tests performed, according the procedures here described. (author)

  16. Evaluating newly acquired authority of nurse practitioners and physician assistants for reserved medical procedures in the Netherlands: a study protocol

    OpenAIRE

    De Bruijn-Geraets, Daisy P; Van Eijk-Hustings, Yvonne JL; Vrijhoef, Hubertus JM

    2014-01-01

    Aim The study protocol is designed to evaluate the effects of granting independent authorization for medical procedures to nurse practitioners and physician assistants on processes and outcomes of health care. Background Recent (temporarily) enacted legislation in Dutch health care authorizes nurse practitioners and physician assistants to indicate and perform specified medical procedures, i.e. catheterization, cardioversion, defibrillation, endoscopy, injection, puncture, prescribing and sim...

  17. A limited assessment of the ASEP human reliability analysis procedure using simulator examination results

    Energy Technology Data Exchange (ETDEWEB)

    Gore, B.R.; Dukelow, J.S. Jr.; Mitts, T.M.; Nicholson, W.L. [Pacific Northwest Lab., Richland, WA (United States)

    1995-10-01

    This report presents a limited assessment of the conservatism of the Accident Sequence Evaluation Program (ASEP) human reliability analysis (HRA) procedure described in NUREG/CR-4772. In particular, the, ASEP post-accident, post-diagnosis, nominal HRA procedure is assessed within the context of an individual`s performance of critical tasks on the simulator portion of requalification examinations administered to nuclear power plant operators. An assessment of the degree to which operator perforn:Lance during simulator examinations is an accurate reflection of operator performance during actual accident conditions was outside the scope of work for this project; therefore, no direct inference can be made from this report about such performance. The data for this study are derived from simulator examination reports from the NRC requalification examination cycle. A total of 4071 critical tasks were identified, of which 45 had been failed. The ASEP procedure was used to estimate human error probability (HEP) values for critical tasks, and the HEP results were compared with the failure rates observed in the examinations. The ASEP procedure was applied by PNL operator license examiners who supplemented the limited information in the examination reports with expert judgment based upon their extensive simulator examination experience. ASEP analyses were performed for a sample of 162 critical tasks selected randomly from the 4071, and the results were used to characterize the entire population. ASEP analyses were also performed for all of the 45 failed critical tasks. Two tests were performed to assess the bias of the ASEP HEPs compared with the data from the requalification examinations. The first compared the average of the ASEP HEP values with the fraction of the population actually failed and it found a statistically significant factor of two bias on the average.

  18. Detector Simulations with DD4hep

    Science.gov (United States)

    Petrič, M.; Frank, M.; Gaede, F.; Lu, S.; Nikiforou, N.; Sailer, A.

    2017-10-01

    Detector description is a key component of detector design studies, test beam analyses, and most of particle physics experiments that require the simulation of more and more different detector geometries and event types. This paper describes DD4hep, which is an easy-to-use yet flexible and powerful detector description framework that can be used for detector simulation and also extended to specific needs for a particular working environment. Linear collider detector concepts ILD, SiD and CLICdp as well as detector development collaborations CALICE and FCal have chosen to adopt the DD4hep geometry framework and its DDG4 pathway to Geant4 as its core simulation and reconstruction tools. The DDG4 plugins suite includes a wide variety of input formats, provides access to the Geant4 particle gun or general particles source and allows for handling of Monte Carlo truth information, eg. by linking hits and the primary particle that caused them, which is indispensable for performance and efficiency studies. An extendable array of segmentations and sensitive detectors allows the simulation of a wide variety of detector technologies. This paper shows how DD4hep allows to perform complex Geant4 detector simulations without compiling a single line of additional code by providing a palette of sub-detector components that can be combined and configured via compact XML files. Simulation is controlled either completely via the command line or via simple Python steering files interpreted by a Python executable. It also discusses how additional plugins and extensions can be created to increase the functionality.

  19. Variability in the recognition of distinctive immunofluorescence patterns in different brands of HEp-2 cell slides

    Directory of Open Access Journals (Sweden)

    Alessandra Dellavance

    2013-06-01

    Full Text Available INTRODUCTION: Indirect immunofluorescence on HEp-2 cells is considered the gold standard for the detection of autoantibodies against cellular antigens. However, the culture conditions, cell fixation and permeabilization processes interfere directly in the preservation and spatial distribution of antigens. Therefore, one can assume that certain peculiarities in the processing of cellular substrate may affect the recognition of indirect immunofluorescence patterns associated with several autoantibodies. OBJECTIVE: To evaluate a panel of serum samples representing nuclear, nucleolar, cytoplasmic, mitotic apparatus, and chromosome plate patterns on HEp-2 cell substrates from different suppliers. MATERIALS AND METHODS: Seven blinded observers, independent from the three selected reference centers, evaluated 17 samples yielding different nuclear, nucleolar, cytoplasmic and mitotic apparatus patterns on HEp-2 cell slides from eight different brands. The slides were coded to maintain confidentiality of both brands and participating centers. RESULTS: The 17 HEp-2 cell patterns were identified on most substrates. Nonetheless, some slides showed deficit in the expression of several patterns: nuclear coarse speckled/U1-ribonucleoprotein associated with antibodies against RNP (U1RNP, centromeric protein F (CENP-F, proliferating cell nuclear antigen (PCNA, cytoplasmic fine speckled associated with anti-Jo-1 antibodies (histidyl synthetase, nuclear mitotic apparatus protein 1 (NuMA-1 and nuclear mitotic apparatus protein 2 (NuMA-2. CONCLUSION: Despite the overall good quality of the assessed HEp-2 substrates, there was considerable inconsistency in results among different commercial substrates. The variations may be due to the evaluated batches, hence generalizations cannot be made as to the respective brands. It is recommended that each new batch or new brand be tested with a panel of reference sera representing the various patterns.

  20. Evaluation of Glove Damage during Dental Procedures among Dental Specialists in Tabriz

    Directory of Open Access Journals (Sweden)

    Saeed Nezafati

    2007-08-01

    Full Text Available

    Background and aims. Dental practitioners are prone to occupational risk of infection. This can be prevented in part by wearing gloves. However, for this to be effective, gloves should be intact during the entire course of dental procedure. Leaky surgical latex gloves have been seen in 0.9% of cases before use. As much as 1.9% of latex gloves have been reported to be damaged during dental procedures. In this study, we decided to assess glove damage during dental procedures among dental specialists in Tabriz.

    Materials and methods. Thirty-six dental specialists were selected for this study. Each practitioner received 40 pairs of intact powdered latex gloves. Upon the completion of dental procedures, the gloves were retrieved and any tears were evaluated separately for right and left hands. Data was analyzed using chi-square test.

    Results. 159 punctures were detected in 144 gloves (5% out of 2880 unpaired gloves used by practitioners. They noticed the tear(s in 60 cases (2%, however, 99 cases (3% of tear(s were not noted during the procedure. The highest rate of glove damage was observed in the prosthodontists’ group (12.3%, which was statistically significant comparing to other groups (p=0.048. The lowest rate of the damage was observed in the oral surgeons’ group (2% which showed no significant difference (p=0.134. The highest rate of punctures in the gloves was observed in the first and second fingers of the non-dominant hand.

    Conclusion. The damage to 5% of the gloves is highly significant, with a potential role in occupational hazards. The higher rate of leaks in the prosthodontists’ group compared to other groups demands for greater prudence in this field. The high rate of leaks in the first and second fingers of the non-dominant hand requires more attention to this area during daily practice.

  1. Biomechanical evaluation against calcaneofibular ligament repair in the Brostrom procedure: a cadaveric study.

    Science.gov (United States)

    Lee, Kyung-Tai; Lee, Jung Il; Sung, Ki Sun; Kim, J-Young; Kim, Eung Soo; Lee, Sang-Heon; Wang, Joon Ho

    2008-08-01

    The modified Brostrom procedure is commonly recommended for reconstruction of the anterior talofibular ligament (ATF) and calcaneofibular ligament (CF) with an advancement of the inferior retinaculum. However, some surgeons perform the modified Bostrom procedure with an semi-single ATF ligament reconstruction and advancement of the inferior retinaculum for simplicity. This study evaluated the initial stability of the modified Brostrom procedure and compared a two ligaments (ATF + CF) reconstruction group with a semi-single ligament (ATF) reconstruction group. Sixteen paired fresh frozen cadaveric ankle joints were used in this study. The ankle joint laxity was measured on the plane radiographs with 150 N anterior drawer force and 150 N varus stress force. The anterior displacement distances and varus tilt angles were measured before and after cutting the ATF and CF ligaments. A two ligaments (ATF + CF) reconstruction with an advancement of the inferior retinaculum was performed on eight left cadaveric ankles, and an semi-single ligament (ATF) reconstruction with an advancement of the inferior retinaculum was performed on eight right cadaveric ankles. The ankle instability was rechecked after surgery. The decreases in instability of the ankle after surgery were measured and the difference in the decrease was compared using a Mann-Whitney U test. The mean decreases in anterior displacement were 3.4 and 4.0 mm in the two ligaments reconstruction and semi-single ligament reconstruction groups, respectively. There was no significant difference between the two groups (P = 0.489). The mean decreases in the varus tilt angle in the two ligaments reconstruction and semi-single ligament reconstruction groups were 12.6 degrees and 12.2 degrees , respectively. There was no significant difference between the two groups (P = 0.399). In this cadaveric study, a substantial level of initial stability can be obtained using an anatomical reconstruction of the anterior talofibular

  2. From Physics to industry: EOS outside HEP

    Science.gov (United States)

    Espinal, X.; Lamanna, M.

    2017-10-01

    In the competitive market for large-scale storage solutions the current main disk storage system at CERN EOS has been showing its excellence in the multi-Petabyte high-concurrency regime. It has also shown a disruptive potential in powering the service in providing sync and share capabilities and in supporting innovative analysis environments along the storage of LHC data. EOS has also generated interest as generic storage solution ranging from university systems to very large installations for non-HEP applications.

  3. Totem a case study in HEP

    CERN Document Server

    Dusini, S; Lazzizzera, I; Lee, P; Sartori, A; Sidoti, A; Tecchiolli, G P; Zorat, A

    1997-01-01

    It is being proved that the neurochip \\Totem{} is a viable solution for high quality and real time computational tasks in HEP, including event classification, triggering and signal processing. The architecture of the chip is based on a "derivative free" algorithm called Reactive Tabu Search (RTS), highly performing even for low precision weights. ISA, VME or PCI boards integrate the chip as a coprocessor in a host computer. This paper presents: 1) the state of the art and the next evolution of the design of \\Totem{}; 2) its ability in the Higgs search at LHC as an example.

  4. Online production validation in a HEP environment

    Science.gov (United States)

    Harenberg, T.; Kuhl, T.; Lang, N.; Mättig, P.; Sandhoff, M.; Schwanenberger, C.; Volkmer, F.

    2017-03-01

    In high energy physics (HEP) event simulations, petabytes of data are processed and stored requiring millions of CPU-years. This enormous demand for computing resources is handled by centers distributed worldwide, which form part of the LHC computing grid. The consumption of such an important amount of resources demands for an efficient production of simulation and for the early detection of potential errors. In this article we present a new monitoring framework for grid environments, which polls a measure of data quality during job execution. This online monitoring facilitates the early detection of configuration errors (specially in simulation parameters), and may thus contribute to significant savings in computing resources.

  5. Evaluating North Carolina Food Pantry Food Safety-Related Operating Procedures.

    Science.gov (United States)

    Chaifetz, Ashley; Chapman, Benjamin

    2015-11-01

    Almost one in seven American households were food insecure in 2012, experiencing difficulty in providing enough food for all family members due to a lack of resources. Food pantries assist a food-insecure population through emergency food provision, but there is a paucity of information on the food safety-related operating procedures used in the pantries. Food pantries operate in a variable regulatory landscape; in some jurisdictions, they are treated equivalent to restaurants, while in others, they operate outside of inspection regimes. By using a mixed methods approach to catalog the standard operating procedures related to food in 105 food pantries from 12 North Carolina counties, we evaluated their potential impact on food safety. Data collected through interviews with pantry managers were supplemented with observed food safety practices scored against a modified version of the North Carolina Food Establishment Inspection Report. Pantries partnered with organized food bank networks were compared with those that operated independently. In this exploratory research, additional comparisons were examined for pantries in metropolitan areas versus nonmetropolitan areas and pantries with managers who had received food safety training versus managers who had not. The results provide a snapshot of how North Carolina food pantries operate and document risk mitigation strategies for foodborne illness for the vulnerable populations they serve. Data analysis reveals gaps in food safety knowledge and practice, indicating that pantries would benefit from more effective food safety training, especially focusing on formalizing risk management strategies. In addition, new tools, procedures, or policy interventions might improve information actualization by food pantry personnel.

  6. Anti-hepatocarcinoma effects of resveratrol nanoethosomes against human HepG2 cells

    Science.gov (United States)

    Meng, Xiang-Ping; Zhang, Zhen; Chen, Tong-sheng; Wang, Yi-fei; Wang, Zhi-ping

    2017-02-01

    Hepatocarcinoma, a malignant cancer, threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma to chemotherapy. Resveratrol (Res) has been widely investigated with its strong anti-tumor activity. However, its low oral bioavailability restricts its wide application. In this study, we prepared resveratrol nanoethosomes (ResN) via ethanol injection method. The in vitro anti-hepatocarcinoma effects of ResN relative to efficacy of bulk Res were evaluated on proliferation and apoptosis of human HepG2 cells. ResN were spherical vesicles and its particle diameter, zeta potential were (115.8 +/- 1.3) nm and (-12.8 +/- 1.9) mV, respectively. ResN exhibited significant inhibitory effects against human HepG2 cells by MTT assay, and the IC50 value was 49.2 μg/ml (105.4 μg/ml of Res bulk solution). By flow cytometry assay, there was an increase in G2/M phase cells treated with ResN. The results demonstrated ResN could effectively block the G2/M phase of HepG2 cells, which can also enhance the inhibitory effect of Res against HepG2 cells.

  7. The role of scientific middleware in the future of HEP computing

    CERN Multimedia

    CERN. Geneva

    2004-01-01

    In the 18 months since the CHEP03 meeting in San Diego, the HEP community deployed the current generation of grid technologies in a veracity of settings. Legacy software as well as recently developed applications was interfaced with middleware tools to deliver end-to-end capabilities to HEP experiments in different stages of their life cycles. In a series of data challenges, reprocessing efforts and data distribution activities the community demonstrated the benefits distributed computing can offer and the power a range of middleware tools can deliver. After running millions of jobs, moving tera-bytes of data, creating millions of files and resolving hundreds of bug reports, the community also exposed the limitations of these middleware tools. As we move to the next level of challenges, requirements and expectations, we must also examine the methods and procedures we employ to develop, implement and maintain our common suite of middleware tools. The talk will focus on the role common middleware ...

  8. Measurement of spatial dose distribution for evaluation operator dose during nero-interventional procedures

    Energy Technology Data Exchange (ETDEWEB)

    Han, Su Chul [Division of Medical Radiation Equipment, Korea Institute of Radiological and Medical Sciences, Seoul (Korea, Republic of); Hong, Dong Hee [Dept. of Radiology Science, Far East University, Eumseong (Korea, Republic of)

    2016-09-15

    The spatial dose distribution was measured with ionization chamber as preliminary study to evaluate operator dose and to study dose reduction during neuro-interventional procedures. The zone of operators was divided into four area (45, 135, 225, and 315 degree).We supposed that operator exist on the four area and indicated location of critical organs(eyes, breast, gonad). The spatial doses were measured depending on distance( 80, 100, 120, and 140 cm) and location of critical organs. The spatial doses of area of 225 degree were 114.5 mR/h (eyes location), 143.1 mR/h (breast location) and 147 mR/h (gonad location) in 80 cm. When changed location of x-ray generator, spatial dose increased in 18.1±10.5%, averagely. We certified spatial dose in the operator locations, Using the results of this study, It is feasible to protect operator from radiation in neuro-interventional procedures.

  9. Evaluation of procedures for the conservation and culture of gastrointestinal parasites from free ranging primates

    Directory of Open Access Journals (Sweden)

    Fernando Nassar Montoya

    2005-05-01

    Full Text Available Obtaining good quality fecal samples from freeranging New World Primates is normally a slow and expensive task. So, it is necessary to ensure good sample handling for the optimisation of preservation and diagnosis. During some parasitological studies carried out by the Centro Araguatos, we obtained larvae from coprocultures and fecal samples preserved in a preservative (MIF. These procedures allowed us to study deeper the parasites of free-ranging monkeys. However, we have found problems: like the rapid desiccation of fecal samples in the coprocultures and some limitations on the implementation of the quantitative diagnostic procedures. This study evaluated the effect, use and limitations of using coprocultures and conserving the parasite eggs and cysts in MIF for the study of parasites in free-ranging New World Primates. The results show that it is possible to implement an efficient methodology for the conservation and parasitological analysis of fecal samples of New World Primates using both coprocultures and eggs conserved in MIF. This enhances the capacity of studies and improves diagnostic possibility. The larvae in the fecal samples show better growth in a proportion of fecal sample: substrate (sawdust of 1:1, in culturing periods ranging from 7 to 15 days. A good dilution of feces: MIF for the conservation of eggs and quists is 1:10.

  10. Detector simulations with DD4hep

    CERN Document Server

    AUTHOR|(SzGeCERN)668365; Frank, Markus; Gaede, Frank-Dieter; Lu, Shaojun; Nikiforou, Nikiforos; Sailer, Andre

    2017-01-01

    Detector description is a key component of detector design studies, test beam analyses, and most of particle physics experiments that require the simulation of more and more different detector geometries and event types. This paper describes DD4hep, which is an easy-to-use yet flexible and powerful detector description framework that can be used for detector simulation and also extended to specific needs for a particular working environment. Linear collider detector concepts ILD, SiD and CLICdp as well as detector development collaborations CALICE and FCal have chosen to adopt the DD4hep geometry framework and its DDG4 pathway to Geant4 as its core simulation and reconstruction tools. The DDG4 plugins suite includes a wide variety of input formats, provides access to the Geant4 particle gun or general particles source and allows for handling of Monte Carlo truth information, e.g. by linking hits and the primary particle that caused them, which is indispensable for performance and efficiency studies. An extend...

  11. Environmental risk assessment for plant pests: a procedure to evaluate their impacts on ecosystem services.

    Science.gov (United States)

    Gilioli, G; Schrader, G; Baker, R H A; Ceglarska, E; Kertész, V K; Lövei, G; Navajas, M; Rossi, V; Tramontini, S; van Lenteren, J C

    2014-01-15

    The current methods to assess the environmental impacts of plant pests differ in their approaches and there is a lack of the standardized procedures necessary to provide accurate and consistent results, demonstrating the complexity of developing a commonly accepted scheme for this purpose. By including both the structural and functional components of the environment threatened by invasive alien species (IAS), in particular plant pests, we propose an environmental risk assessment scheme that addresses this complexity. Structural components are investigated by evaluating the impacts of the plant pest on genetic, species and landscape diversity. Functional components are evaluated by estimating how plant pests modify ecosystem services in order to determine the extent to which an IAS changes the functional traits that influence ecosystem services. A scenario study at a defined spatial and temporal resolution is then used to explore how an IAS, as an exogenous driving force, may trigger modifications in the target environment. The method presented here provides a standardized approach to generate comparable and reproducible results for environmental risk assessment as a component of Pest Risk Analysis. The method enables the assessment of overall environmental risk which integrates the impacts on different components of the environment and their probabilities of occurrence. The application of the proposed scheme is illustrated by evaluating the environmental impacts of the invasive citrus long-horn beetle, Anoplophora chinensis. © 2013.

  12. Fetus absorbed dose evaluation in head and neck radiotherapy procedures of pregnant patients

    Energy Technology Data Exchange (ETDEWEB)

    Camargo da C, E.; Ribeiro da R, L. A.; Santos B, D. V., E-mail: etieli@ird.gov.br [Instituto de Radioprotecao e Dosimetria / CNEN, Av. Salvador Allende s/n, Barra de Tijuca, 22783-127 Rio de Janeiro (Brazil)

    2014-08-15

    Each year a considerable amount of pregnant women needs to be submitted to radiotherapeutic procedures to combat malignant tumors. Radiation therapy is often a treatment of choice for these patients. It is possible to use shielding and beam positioning such that the potential dose to the fetus can be minimized. In this work the head and neck cancer treatment of a pregnant patient was experimentally simulated. The patient was simulated by an anthropomorphic Alderson phantom and the absorbed dose to the fetus was evaluated using micro-rod TLD-100 detectors in two conditions, namely protecting the patients abdomen with a 7 cm lead layer and using no abdomen shielding. The aim of this experiment was to evaluate the efficiency of the abdomen protection in reducing the fetus absorbed dose. Irradiations were performed with a Trilogy linear accelerator using x-rays of 6 MV. A total dose of 50 Gy to the target volume was delivered. The fetus doses evaluated with and without the lead shielding were, respectively, 0.52±0.039 and (0.88±0.052) c Gy, corresponding to a dose reduction of 59%. The dose (0.52±0.039) c Gy is within the zone of biological tolerance for the fetus. (Author)

  13. Evaluating procedural modelling for 3D models of informal settlements in urban design activities

    Directory of Open Access Journals (Sweden)

    Victoria Rautenbach

    2015-11-01

    Full Text Available Three-dimensional (3D modelling and visualisation is one of the fastest growing application fields in geographic information science. 3D city models are being researched extensively for a variety of purposes and in various domains, including urban design, disaster management, education and computer gaming. These models typically depict urban business districts (downtown or suburban residential areas. Despite informal settlements being a prevailing feature of many cities in developing countries, 3D models of informal settlements are virtually non-existent. 3D models of informal settlements could be useful in various ways, e.g. to gather information about the current environment in the informal settlements, to design upgrades, to communicate these and to educate inhabitants about environmental challenges. In this article, we described the development of a 3D model of the Slovo Park informal settlement in the City of Johannesburg Metropolitan Municipality, South Africa. Instead of using time-consuming traditional manual methods, we followed the procedural modelling technique. Visualisation characteristics of 3D models of informal settlements were described and the importance of each characteristic in urban design activities for informal settlement upgrades was assessed. Next, the visualisation characteristics of the Slovo Park model were evaluated. The results of the evaluation showed that the 3D model produced by the procedural modelling technique is suitable for urban design activities in informal settlements. The visualisation characteristics and their assessment are also useful as guidelines for developing 3D models of informal settlements. In future, we plan to empirically test the use of such 3D models in urban design projects in informal settlements.

  14. II - Multivariate Classification and Machine Learning in HEP

    CERN Multimedia

    CERN. Geneva

    2016-01-01

    A summary of the history of deep-learning is given and the difference to traditional artificial neural networks is discussed. Advanced methods like convoluted neural networks, recurrent neural networks and unsupervised training are introduced. Interesting examples from this emerging field outside HEP are presented. Possible applications in HEP are discussed.

  15. Evaluation procedure of software safety plan for digital I and C of KNGR

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jang Soo; Park, Jong Kyun; Lee, Ki Young; Kwon, Ki Choon; Kim, Jang Yeol; Cheon, Se Woo

    2000-05-01

    The development, use, and regulation of computer systems in nuclear reactor instrumentation and control (I and C) systems to enhance reliability and safety is a complex issue. This report is one of a series of reports from the Korean next generation reactor (KNGR) software safety verification and validation (SSVV) task, Korea Atomic Energy Research Institute, which investigates different aspects of computer software in reactor I and C systems, and describes the engineering procedures for developing such a software. The purpose of this guideline is to give the software safety evaluator the trail map between the code and standards layer and the design methodology and documents layer for the software important to safety in nuclear power plants. Recently, the safety planning for safety-critical software systems is being recognized as the most important phase in the software life cycle, and being developed new regulatory positions and standards by the regulatory and the standardization organizations. The requirements for software important to safety of nuclear reactor are described in such positions and standards, for example, the new standard review plan (SRP), IEC 880 supplements, IEEE standard 1228-1994, IEEE standard 7-4.3.2-1993, and IAEA safety series No. 50-SG-D3 and D8. We presented the guidance for evaluating the safety plan of the software in the KNGR protection systems. The guideline consists of the regulatory requirements for software safety in chapter 2, the evaluation checklist of software safety plan in chapter3, and the evaluation results of KNGR software safety plan in chapter 4.

  16. CLINICAL AND RADIOLOGICAL EVALUATION ON DEVELOPMENTAL HIP DYSPLASIA AFTER SALTER AND OMBRÉDANNE PROCEDURE

    Science.gov (United States)

    da Rocha, Válney Luiz; Thomé, André Luiz Coelho; da Silva Castro, Daniel Labres; de Oliveira, Leandro Zica; de Moraes, Frederico Barra

    2015-01-01

    Objective: To evaluate the clinical and radiological medium-term results from surgical treatment of developmental hip dysplasia through Salter innominate bone osteotomy and Ombrédanne femoral shortening. Methods: Fourteen patients were evaluated, with surgical treatment on 18 hips (seven right-side hips and eleven left-side hips) using the proposal technique, performed between 1998 and 2008. The Dutoit and Severin criteria were used respectively for clinical and radiographic evaluations. Results: The average preoperative index for the seven right-side hips was 43.3° (40° to 50°), and this was corrected through surgery to an average of 31.57° (24° to 42°). The average preoperative index for the eleven left-side hips was 42.1° (36° to 56°), and this was corrected through surgery to an average of 30.36° (20° to 44°). There was a statistically significant difference between the preoperative and postoperative acetabular indexes, with P > 0.05. The clinical evaluation showed that there were seven excellent hips (38.9%), eight good ones (44.4%), three fair hips (16.7%) and no poor ones (0%). By grouping the hips rated good and excellent as satisfactory and those rated poor and fair as unsatisfactory, 83.3% of the results were seen to be favorable. There were no statistically significant correlations between occurrences of complications and patient age at the time of surgery or between complications and the preoperative acetabular index (p > 0.05). The complications observed consisted of one case each of subluxation, osteonecrosis and osteonecrosis together with subluxation. Conclusion: The combined procedure of Salter and Ombrédanne is a viable option for treating developmental hip dysplasia after patients have started to walk. PMID:27027068

  17. Extracellular visfatin activates gluconeogenesis in HepG2 cells through the classical PKA/CREB-dependent pathway.

    Science.gov (United States)

    Choi, Y J; Choi, S-E; Ha, E S; Kang, Y; Han, S J; Kim, D J; Lee, K W; Kim, H J

    2014-04-01

    Adipokines reportedly affect hepatic gluconeogenesis, and the adipokine visfatin is known to be related to insulin resistance and type 2 diabetes. However, whether visfatin contributes to hepatic gluconeogenesis remains unclear. Visfatin, also known as nicotinamide phosphoribosyltransferase (NAMPT), modulates sirtuin1 (SIRT1) through the regulation of nicotinamide adenine dinucleotide (NAD). Therefore, we investigated the effect of extracellular visfatin on glucose production in HepG2 cells, and evaluated whether extracellular visfatin affects hepatic gluconeogenesis via an NAD+-SIRT1-dependent pathway. Treatment with visfatin significantly increased glucose production and the mRNA expression and protein levels of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) in HepG2 cells in a time- and concentration-dependent manner. Knockdown of SIRT1 had no remarkable effect on the induction of gluconeogenesis by visfatin. Subsequently, we evaluated if extracellular visfatin stimulates the production of gluconeogenic enzymes through the classical protein kinase A (PKA)/cyclic AMP-responsive element (CRE)-binding protein (CREB)-dependent process. The phosphorylation of CREB and PKA increased significantly in HepG2 cells treated with visfatin. Additionally, knockdown of CREB and PKA inhibited visfatin-induced gluconeogenesis in HepG2 cells. In summary, extracellular visfatin modulates glucose production in HepG2 cells through the PKA/CREB pathway, rather than via SIRT1 signaling. © Georg Thieme Verlag KG Stuttgart · New York.

  18. Rheological evaluation of polymers for EOR. Proper procedures for a laboratory approach

    Energy Technology Data Exchange (ETDEWEB)

    Kouchaki, S.; Hincapie-Reina, R.; Ganzer, L. [Technische Univ. Clausthal, Clausthal-Zellerfeld (Germany). ITE

    2013-08-01

    Some discrepancies exist in the literature concerning polymer EOR (Enhanced Oil Recovery) evaluation. Laboratory investigations are essentials for every particular flooding project, with proper preliminary lab evaluation techniques in order to study the polymer behavior and rheological properties at different reservoir conditions. Different research has been shown variation in procedures during polymer preparation and filtration; affecting the result in the field. Experience from field observation indicates that even high molecular weight (MW) polymers have filtration ratio (F.R) less than 1,5; in that way polymer solutions that are still in range cannot show a good approximation to the field behavior. To deal with the discrepancies, specific procedures based in rheological polymer characterization were adapted. Two different polymers were used for rheology study using a rotational rheometer analyzing some solutions at different conditions. Additionally rheological measurements was used to characterize the apparent viscosity of polymer as a shear rate function, salinity, temperature and polymer concentration, defining additional useful values for simulation evaluations and additionally Newtonian, non-Newtonian, shear viscosity and MW impact were also investigated. Quality control of the solutions was not limited to the conventional indicator 'filtrations ratio', but it was showed how important it is to consider two qualities check indicators, filterability ratio and filterability plot, simultaneously. Results indicated how stable the N-vinyl Pyrrolidone (NVP) Superpusher SAV301 could be in comparison to the Hydrolyzed Polyacrylamide polymer (HPAM) Flopaam 3630S. Despite the high viscosity results from HPAM Flopaam 3630S due to its high MW, the polymer is overly sensitive to factors like temperature, salinity and concentration. Quite the contrary was for polymer solutions with Superpusher SAV301; constant viscosity over a wide shear rate range which is the

  19. Evaluation of two PCR-based procedures for typing Clostridium perfringens : research communication

    Directory of Open Access Journals (Sweden)

    B. Dungu

    2000-07-01

    Full Text Available Two polymerase chain reaction (PCR-based procedures for typing Clostridium perfringens, which affects most domestic animals, were compared and evaluated for efficiency as substitute to the guinea-pig intradermal test routinely used in our laboratory, namely a multiplex PCR and a protocol based on the individual amplification of gene sequences specific for each toxin. Reference isolates of C. perfringens types A, B, C and D as well as cultures from clinical specimens were tested. The sensitivity and specificity of the PCR was confirmed on reference isolates. There was similarity in results on 43 of the 46 samples typed by all 3 methods. Clear results were obtained by PCR on 5 clinical samples that showed either equivocal or weak skin reactions in guinea-pigs. The multiplex PCR protocol, in combination with the evaluation of bacterial growth, is a better alternative to in vivo toxin typing, since C. perfringens can only be incriminated as cause of a disease when it is present in large numbers in the intestine.

  20. What does it take to show that a cognitive training procedure is useful? A critical evaluation.

    Science.gov (United States)

    Jacoby, Nori; Ahissar, Merav

    2013-01-01

    Individuals substantially improve with training, indicating that a large degree of plasticity is retained across ages. In the past 20 years, many studies explored the ability to boost cognitive skills (reasoning, linguistic abilities, working memory, and attention) by training with other tasks that exploit limited cognitive resources. Indeed, individuals with long-term training on challenging skills (musicians and action video gamers) show impressive behavior on related tasks (linguistic and visual attention, respectively). However, a critical evaluation of training studies that last weeks to months shows typically mild effects, mainly with respect to control groups that either did not practice or practiced with less challenging, rewarding, or exciting conditions. These findings suggest that future training studies should evaluate these factors carefully and assess whether they mainly impact the testing sessions or actual longer-term skills, and whether their impact can be further strengthened. The lack of a comprehensive theory of learning that integrates cognitive, motivational, and alertness aspects poses a bottleneck to improving current training procedures. © 2013 Elsevier B.V. All rights reserved.

  1. Evaluation of accuracy in implant site preparation performed in single- or multi-step drilling procedures.

    Science.gov (United States)

    Marheineke, Nadine; Scherer, Uta; Rücker, Martin; von See, Constantin; Rahlf, Björn; Gellrich, Nils-Claudius; Stoetzer, Marcus

    2017-12-17

    Dental implant failure and insufficient osseointegration are proven results of mechanical and thermal damage during the surgery process. We herein performed a comparative study of a less invasive single-step drilling preparation protocol and a conventional multiple drilling sequence. Accuracy of drilling holes was precisely analyzed and the influence of different levels of expertise of the handlers and additional use of drill template guidance was evaluated. Six experimental groups, deployed in an osseous study model, were representing template-guided and freehanded drilling actions in a stepwise drilling procedure in comparison to a single-drill protocol. Each experimental condition was studied by the drilling actions of respectively three persons without surgical knowledge as well as three highly experienced oral surgeons. Drilling actions were performed and diameters were recorded with a precision measuring instrument. Less experienced operators were able to significantly increase the drilling accuracy using a guiding template, especially when multi-step preparations are performed. Improved accuracy without template guidance was observed when experienced operators were executing single-step versus multi-step technique. Single-step drilling protocols have shown to produce more accurate results than multi-step procedures. The outcome of any protocol can be further improved by use of guiding templates. Operator experience can be a contributing factor. Single-step preparations are less invasive and are promoting osseointegration. Even highly experienced surgeons are achieving higher levels of accuracy by combining this technique with template guidance. Hereby template guidance enables a reduction of hands-on time and side effects during surgery and lead to a more predictable clinical diameter.

  2. Evaluation of a modified "Triple-P" procedure in women with morbidly adherent placenta after previous caesarean section.

    Science.gov (United States)

    Wei, Yanxing; Cao, Yanwen; Yu, Yanhong; Wang, Zhijian

    2017-07-21

    To describe a modified "Triple-P" procedure and evaluate its outcome in women with morbidly adherent placenta (MAP) after previous caesarean section (CS). A retrospective cohort study of 96 women with MAP after CS was recruited with 45 women receiving the modified "Triple-P" procedure as study group and the other 51 cases receiving the conventional managements as the control. The maternal outcomes were compared. The modified "Triple-P" procedure was described in step by step. Women in study group demonstrated reduction of blood loss, transfusion blood volume and operation time, as well as less hospital days and lower hospitalization cost (P P > 0.05). Our modified "Triple-P" procedure for MAP after previous CS maintained the advantages of Chandraharan's "Triple-P" procedure in preservation of uterus for further fertility, less intraoperative blood loss, shorter hospital stays, and lower hospitalization cost but also advanced in feasibility and convenience during introducing into routine clinical practice.

  3. A Screening Procedure for Evaluating Cotton for Rotylenchulus reniformis Resistance in Controlled Conditions

    Directory of Open Access Journals (Sweden)

    Konan, NO.

    2014-01-01

    Full Text Available Rotylenchulus reniformis is one of the most important nematode pests of cotton. Currently, no cotton cultivar resistant cotton cultivar to this pest is available. In the framework of a breeding program aiming at producing cotton genotype resistant to this nematode, a R. reniformis resistance evaluation protocol based on egg inoculation, extraction and counting, has been established. Two environment conditions (growth chamber and greenhouse, four sieves (75, 50, 20 and 10 µm, three doses of inoculum (1,500; 3,000 and 6,000 eggs, and five durations of infestation (20, 30, 40, 50 and 60 days after inoculation have been tested. The growth chamber programmed to provide 12 h of light, 55%-60% relative humidity and 30-26°C day-night air temperatures revealed to be adequate. The NaOCl (sodium hypochloride-blender-sieving-centrifugation-flotation method, using 75-µm and 20-µm sieves, kaolin powder and MgSO4 (magnesium sulfate solution (specific gravity 1.18 proved to be suitable for effective R. reniformis egg extraction (from roots and counting. Inoculation of 6,000 eggs per seedling and 60 days duration of infestation seemed to be sufficient dose and period for a reliable resistance evaluation. The protocol developed has been tested on known susceptible and resistant cotton genotypes: G. hirsutum L (main cultivated species through the world, susceptible, G. thurberi Tod. (wild cotton species, moderately susceptible G. longicalyx Hutch. and Lee (wild cotton species, very resistant. The results obtained were in accordance with the response expected from the genotypes tested, proving the reliability of the evaluation procedure developed.

  4. Evaluation of the Indoor Air Quality Procedure for Use in Retail Buildings

    Energy Technology Data Exchange (ETDEWEB)

    Dutton, Spencer M. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Chan, Wanyu R. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Mendell, Mark J. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Barrios, Marcella [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Parthasarathy, Srinandini [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sidheswaran, Meera [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sullivan, Douglas P. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Eliseeva, Katerina [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Fisk, William J. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2013-02-01

    California's building efficiency standards (Title 24) mandate minimum prescribed ventilation rates (VRs) for commercial buildings. Title 24 standards currently include a prescriptive procedure similar to ASHRAE’s prescriptive “ventilation rate procedure”, but does not include an alternative procedure, akin to ASHRAE’s non-prescriptive “indoor air quality procedure” (IAQP). The IAQP determines minimum VRs based on objectively and subjectively evaluated indoor air quality (IAQ). The first primary goal of this study was to determine, in a set of California retail stores, the adequacy of Title 24 VRs and observed current measured VRs in providing the level of IAQ specified through an IAQP process, The second primary goal was to evaluate whether several VRs implemented experimentally in a big box store would achieve adequate IAQ, assessed objectively and subjectively. For the first goal, a list of contaminants of concern (CoCs) and reference exposure levels (RELs) were selected for evaluating IAQ. Ventilation rates and indoor and outdoor CoC concentrations were measured in 13 stores, including one “big box” store. Mass balance models were employed to calculate indoor contaminant source strengths for CoCs in each store. Using these source strengths and typical outdoor air contaminant concentrations, mass balance models were again used to calculate for each store the “IAQP” VR that would maintain indoor CoC concentrations below selected RELs. These IAQP VRs were compared to the observed VRs and to the Title 24- prescribed VRs. For the second goal, a VR intervention study was performed in the big box store to determine how objectively assessed indoor contaminant levels and subjectively assessed IAQ varied with VR. The three intervention study VRs included an approximation of the store’s current VR [0.24 air changes per hour (ACH)], the Title 24-prescribed VR [0.69 ACH], and the calculated IAQPbased VR [1.51 ACH]). Calculations of IAQP-based VRs

  5. A simple procedure to evaluate the performance of fats and oils at frying temperatures.

    Directory of Open Access Journals (Sweden)

    Barrera-Arellano, D.

    1997-08-01

    Full Text Available A standard procedure is proposed to evaluate the performance of fats and oils at frying temperatures, taking the advantages provided by the Rancimat apparatus, i. e., standard vessels, temperature correction and temperature homogenity in all vessels resulting from the particular characteristics of the heating block. The results obtained in oil samples of 8 g heated at 180° C for 10 h in triplicate gave coefficients of variation lower than 6% for total polar compounds and polymers. In case of limited amount of oil, it is additionally proposed to use only 2 g of sample provided that a similar surface-to-oil volume ratio is maintained, and coefficients of variation of the same order than those for 8 g samples were thus obtained. Advantages of the procedure as well as potential applications for evaluation of frying fats and oils are Included. As an example, the effect of α-tocopherol on performance of sunflower oils was analyzed.

    Se propone un procedimiento estándar para evaluar el comportamiento de aceites y grasas a temperaturas de fritura. En este procedimiento se utilizan las ventajas del aparato Rancimat, que permite el uso de tubos estándar, la corrección de la temperatura, en su caso, y la igualdad de temperatura en todos los tubos dadas las características del bloque de calentamiento. De los resultados obtenidos en muestras de 8 g de aceite calentadas a 180° C durante 10 h, analizadas por triplicado, se obtuvieron coeficientes de variación inferiores al 6% para la determinación de compuestos polares y polímeros. En caso de limitación en la cantidad de aceite, se propone utilizar 2 g de muestra, manteniendo similares valores para la relación superficie a volumen de aceite, lo que permite obtener valores de alteración y coeficientes de variación del mismo orden. Se analizan finalmente las ventajas globales del procedimiento y sus distintas posibilidades en la evaluación de grasas de fritura. Como ejemplo, se aplica el

  6. Comparative evaluation of analytical procedures for the recovery of Enterobacteriaceae in coastal marine waters; Valutazione comparativa di procedure analitiche per il rilevamento di Enterobacteriaceae in acque marine costiere

    Energy Technology Data Exchange (ETDEWEB)

    Bonadonna, Lucia; Chiaretti, Gianluca; Coccia, Anna Maria; Semproni, Maurizio [Istituto Superiore di Sanita`, Rome (Italy). Lab. di Igiene Ambientale

    1997-03-01

    The use of quick and reliable procedures is fundamental for water quality evaluation control. In order to improve the analytical methods for microbiological examination of bathing waters, a comparison of different substrates for the recovery of Enterobacteriaceae from coastal marine waters was carried out. The medium indicated in the Italian technical normative has shown a good selectivity when the red colonies with a green metallic surface sheen were counted, as stated in the Standard Methods. On the other hand, the chronogenic substrate used in this study resulted easy to read, selective and specific for both Escherichia coli and total coliforms.

  7. Trombinol, a bioactive fraction of Psidium guajava, stimulates thrombopoietin expression in HepG2 cells

    OpenAIRE

    Berlian, Guntur; Tandrasasmita, Olivia Mayasari; Tjandrawinata, Raymond Rubianto

    2017-01-01

    Objective: To study the regulation of trombinol on thrombopoietin, an essential regulator of thrombocyte production. Methods: Effect of trombinol on thrombopoietin regulation was evaluated at the mRNA and protein levels in human hepatoma HepG2 cells. The mRNA expressions were revealed by PCR and real-time PCR, while the protein expressions were analyzed using western blotting and human ELISA kit. Statistical differences between the test were determined by student's t-test with P 

  8. Evaluation of exposure dose to patients undergoing catheter ablation procedures - a phantom study

    Energy Technology Data Exchange (ETDEWEB)

    Seguchi, S. [Nagoya University, Graduate School of Medicine, Nagoya (Japan); Nagoya Daini Red Cross Hospital, Division of Radiology, Department of Medical Technology, Nagoya (Japan); Aoyama, T.; Koyama, S.; Kawaura, C. [Nagoya University, Graduate School of Medicine, Nagoya (Japan); Fujii, K. [Nagoya University, Graduate School of Medicine, Nagoya (Japan); National Institute of Radiological Sciences, Section of Radiological Protection, Chiba (Japan)

    2008-11-15

    The aim of this study was to evaluate entrance skin dose (ESD), organ dose and effective dose to patients undergoing catheter ablation for cardiac arrhythmias, based on the dosimetry in an anthropomorphic phantom. ESD values associated with mean fluoroscopy time and digital cine frames were in a range of 0.12-0.30 Gy in right anterior oblique (RAO) and 0.05-0.40 Gy in left anterior oblique (LAO) projection, the values which were less than a threshold dose of 2 Gy for the onset of skin injury. Organs that received high doses in ablation procedures were lung, followed by bone surface, esophagus, liver and red bone marrow. Doses for lung were 24.8-122.7 mGy, and effective doses were 7.9-34.8 mSv for mean fluoroscopy time of 23.4-92.3 min and digital cine frames of 263-511. Conversion coefficients of dose-area product (DAP) to ESD were 8.7 mGy/(Gy.cm{sup 2}) in RAO and 7.4 mGy/(Gy.cm{sup 2}) in LAO projection. The coefficients of DAP to the effective dose were 0.37 mSv/(Gy.cm{sup 2}) in RAO, and 0.41 mSv/(Gy.cm{sup 2}) in LAO projection. These coefficients enabled us to estimate patient exposure in real time by using monitored values of DAP. (orig.)

  9. Critical evaluation of distillation procedure for the determination of methylmercury in soil samples.

    Science.gov (United States)

    Perez, Pablo A; Hintelman, Holger; Quiroz, Waldo; Bravo, Manuel A

    2017-11-01

    In the present work, the efficiency of distillation process for extracting monomethylmercury (MMHg) from soil samples was studied and optimized using an experimental design methodology. The influence of soil composition on MMHg extraction was evaluated by testing of four soil samples with different geochemical characteristics. Optimization suggested that the acid concentration and the duration of the distillation process were most significant and the most favorable conditions, established as a compromise for the studied soils, were determined to be a 70 min distillation using an 0.2 M acid. Corresponding limits of detection (LOD) and quantification (LOQ) were 0.21 and 0.7 pg absolute, respectively. The optimized methodology was applied with satisfactory results to soil samples and was compared to a reference methodology based on isotopic dilution analysis followed by gas chromatography-inductively coupled plasma mass spectrometry (IDA-GC-ICP-MS). Using the optimized conditions, recoveries ranged from 82 to 98%, which is an increase of 9-34% relative to the previously used standard operating procedure. Finally, the validated methodology was applied to quantify MMHg in soils collected from different sites impacted by coal fired power plants in the north-central zone of Chile, measuring MMHg concentrations ranging from 0.091 to 2.8 ng g-1. These data are to the best of our knowledge the first MMHg measurements reported for Chile. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Evaluation of Patient Perceptions After Vestibuloplasty Procedure: A Comparison of Diode Laser and Scalpel Techniques

    Science.gov (United States)

    Farista, Sana; Koppolu, Pradeep; Baroudi, Kusai; Uppada, Udaykiran; Mishra, Ashank; Savarimath, Abhishek; Lingam, Amara Swapna

    2016-01-01

    Introduction Inadequate vestibular depth results in poor plaque control owing to an insufficient width of keratinized gingiva. Vestibuloplasty provides the necessary vestibular depth and can be performed either with a scalpel, electrocautery or lasers. Aim To evaluate the patient perceptions related to pain and discomfort on the 1st, 3rd and the 7th day post vestibuloplasty and also assess the healing outcomes related to the treatment of inadequate vestibular depth either with the diode laser or the scalpel. Materials and Methods Twenty patients who had inadequate vestibular depth and required vestibuloplasty were assigned randomly to undergo the procedure either with the scalpel or the laser. The data obtained was analysed for intergroup comparison with an independent paired t-test and intragroup comparison was determined by a paired t-test. Results Intragroup comparison within the laser group for VAS scores of pain and discomfort within all the reported days exhibited a significant difference (plaser group had lower VAS cores for pain and discomfort compared to the scalpel group (plaser group exhibited better healing outcomes on the 1st, 3rd and the 7th day compared to the scalpel group. Conclusion Observations from the study highlight the opinion that laser can be a safe and effective alternative to traditional vestibuloplasty performed with the scalpel. PMID:27437370

  11. A Comprehensive Procedure to Evaluate the In Vivo Performance of Cancer Nanomedicines.

    Science.gov (United States)

    Tang, Jun; Pérez-Medina, Carlos; Zhao, Yiming; Sadique, Ahmad; Mulder, Willem J M; Reiner, Thomas

    2017-03-04

    Inspired by the success of previous cancer nanomedicines in the clinic, researchers have generated a large number of novel formulations in the past decade. However, only a small number of nanomedicines have been approved for clinical use, whereas the majority of nanomedicines under clinical development have produced disappointing results. One major obstacle to the successful clinical translation of new cancer nanomedicines is the lack of an accurate understanding of their in vivo performance. This article features a rigorous procedure to characterize the in vivo behavior of nanomedicines in tumor-bearing mice at systemic, tissue, single-cell, and subcellular levels via the integration of positron emission tomography-computed tomography (PET-CT), radioactivity quantification methods, flow cytometry, and fluorescence microscopy. Using this approach, researchers can accurately evaluate novel nanoscale formulations in relevant mouse models of cancer. These protocols may have the ability to identify the most promising cancer nanomedicines with high translational potential or to aid in the optimization of cancer nanomedicines for future translation.

  12. Evaluation of relevant information for optimal reflector modeling through data assimilation procedures

    Directory of Open Access Journals (Sweden)

    Argaud Jean-Philippe

    2015-01-01

    Full Text Available The goal of this study is to look after the amount of information that is mandatory to get a relevant parameters optimisation by data assimilation for physical models in neutronic diffusion calculations, and to determine what is the best information to reach the optimum of accuracy at the cheapest cost. To evaluate the quality of the optimisation, we study the covariance matrix that represents the accuracy of the optimised parameter. This matrix is a classical output of the data assimilation procedure, and it is the main information about accuracy and sensitivity of the parameter optimal determination. From these studies, we present some results collected from the neutronic simulation of nuclear power plants. On the basis of the configuration studies, it has been shown that with data assimilation we can determine a global strategy to optimise the quality of the result with respect to the amount of information provided. The consequence of this is a cost reduction in terms of measurement and/or computing time with respect to the basic approach.

  13. A Simple Procedure for the Evaluation of Bone Vitality by Staining with a Tetrazolium Salt

    Directory of Open Access Journals (Sweden)

    René Schiffner

    2017-07-01

    Full Text Available Presently, no intra-operative method for a direct assessment of bone vitality exists. Therefore, we set out to test the applicability of tetrazolium-based staining on bone samples. The explanted femoral heads of 37 patients were used to obtain either cancellous bone fragments or bone slices. Samples were stained with 2,3,5-triphenyl-2H-tetrazolium chloride (TTC or 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (thiazolyl blue, MTT at different times (one to twelve hours after explantation. Staining was quantified either spectrophotometrically after extraction of the dyes or by densitometric image analysis. TTC-staining of cancellous bone fragments and bone slices, respectively, indicated the detectability of vital cells in both types of samples in a window of up to six hours after explantation. Staining intensity at later time-points was indistinguishable from the staining of untreated samples or sodium azide treated samples, which represent dead cells. In contrast, MTT-staining of bone slices revealed intense unspecific staining, which obscured the evaluation of the vitality of the samples. The lack of a detectable increase of colour intensity in TTC-stained bone samples, which were treated more than six hours after explantation, corresponds to reduced fracture healing. The described simple procedure could provide a basis for an intraoperative decision by the orthopaedic surgeon.

  14. Quantitative Evaluation of Contamination on Dental Zirconia Ceramic by Silicone Disclosing Agents after Different Cleaning Procedures

    Directory of Open Access Journals (Sweden)

    Sebastian Wille

    2015-05-01

    Full Text Available The aim of this study was to evaluate the effectiveness of cleaning procedures for air-abraded zirconia after contamination with two silicone disclosing agents. Air-abraded zirconia ceramic specimens (IPS e.max ZirCAD were contaminated with either GC Fit Checker white or GC Fit Checker II. Untreated zirconia specimens were used as control. Afterwards the surfaces were cleaned either with waterspray or ultrasonically in 99% isopropanol or using a newly developed cleaning paste (Ivoclean. After cleaning X-ray photoelectron spectroscopy (XPS was performed and the relative peak intensities of Zr, C and Si were used for a qualitative comparison of the residuals. There was no significant difference between the two different silicone disclosing agents. An additional cleaning step with isopropanol led to a significantly lower amount of residuals on the surface, but an additional cleaning process with Ivoclean did not reduce the amount of carbon residuals in comparison to the isopropanol cleaning. Just the silicone amount on the surface was reduced. None of the investigated cleaning processes removed all residuals from the contaminated surface. Standard cleaning processes do not remove all residuals of the silicone disclosing agent from the surface. This may lead to a failure of the resin-ceramic bonding.

  15. Procedure for the energy evaluation of electric motors; Procedimiento para la evaluacion energetica de motores electricos

    Energy Technology Data Exchange (ETDEWEB)

    Feliz Quiroz, Marco Antonio [Universidad Autonoma Metropolitana (Mexico)

    2002-06-01

    The present article is not a complete treaty about motors, here it is simply tried to show a practical procedure to evaluate electric motors, in order to determine the economics of their possible replacement by others of high efficiency. With the former in mind, the included database is sustained in the information of a representative number of motors brands commercialized in Mexico, since to include all the brands sold in our country would not be practical and it would be outside the scope of this publication. [Spanish] El presente articulo no es todo un tratado acerca de motores, sencillamente aqui se pretende mostrar un procedimiento practico para evaluar los motores electricos, a fin de determinar la rentabilidad de su eventual reemplazo por otros de alta eficiencia. Por lo anterior, la base de datos incluida se sustente en la informacion de un numero representativo de marcas de motores comercializados en Mexico, ya que abarcar todas las marcas vendidas en nuestro pais no seria practico y quedaria fuera del alcance de esta publicacion.

  16. A new test procedure to evaluate the performance of substations for collective heating systems

    Science.gov (United States)

    Baetens, Robin; Verhaert, Ivan

    2017-11-01

    The overall heat demand of a single dwelling, existing out of space heating and domestic hot water production, decreases due to higher insulation rates. Because of this, investing in efficient and renewable heat generation becomes less interesting. Therefore, to incorporate renewables or residual heat on a larger scale, district heating or collective heating systems grow in importance. Within this set-up, the substation is responsible for the interaction between local demand for comfort and overall energy performance of the collective heating system. Many different configurations of substations exist, which influence both local comfort and central system performance. Next to that, also hybrids exist with additional local energy input. To evaluate performance of such substations, a new experimental-based test procedure is developed in order to evaluate these different aspects, characterized by the two roles a substation has, namely as heat generator and as heat consumer. The advantage of this approach is that an objective comparison between individual and central systems regarding performance on delivering local comfort can be executed experimentally. The lab set-up consists out of three different subsystems, namely the central system, the domestic hot water consumption and the local space heating. The central system can work with different temperature regimes and control strategies, as these aspects have proven to have the largest influence on actual performance. The domestic hot water system is able to generate similar tap profiles according to eco-design regulation for domestic hot water generation. The space heating system is able to demand a modular heat load.

  17. Evaluation of Procedural Simulation as a Training and Assessment Tool in General Surgery

    DEFF Research Database (Denmark)

    Bjerrum, Flemming; Strandbygaard, Jeanett; Rosthøj, Susanne

    2017-01-01

    BACKGROUND: Laparoscopic appendectomy is a commonly performed surgical procedure, but few training models have been described for it. We examined a virtual reality module for practising a laparoscopic appendectomy. METHODS: A prospective cohort study with the following 3 groups of surgeons (n = 4...... be useful as a training tool, but further development is required before it can be used for assessment purposes. Procedural simulation may demonstrate more variation for movement parameters, and future research should focus on developing better procedure-specific parameters....

  18. Hemangioma hepático gigante roto Giant hepatic hemangioma with spontaneous rupture

    Directory of Open Access Journals (Sweden)

    Paulo Cezar Galvão do Amaral

    2009-06-01

    Full Text Available INTRODUÇÃO: Ruptura por hemangioma hepático é rara e há somente 32 casos descritos na literatura. RELATO DO CASO: Homem com 39 anos foi admitido com dor abdominal em hipocôndrio direito de início súbito associado à lipotímia. A investigação inicial demonstrou hemangioma hepático gigante (7x13 cm em lobo direito associado a sinais de sangramento recente. Com o intuito de minimizar possibilidade de sangramento no intra-operatório, foi realizado embolização da artéria hepática direita. A operação transcorreu sem intercorrências, sem necessidade de hemotransfusão no intra-operatório. A transecção foi realizada com grampeador linear cortante de 75 mm. O tempo de internação foi de 13 dias. CONCLUSÃO: Procedimento cirúrgico é mandatório para hemangioma hepático roto, e a embolização é eficiente para controlar o sangramento e preparar melhor o paciente para a operação.BACKGROUND: Hepatic hemangioma rupture is rare and there are only 32 cases in the literature. CASE REPORT: A 39-years-old man was admitted for sudden severe upper abdominal pain. Examination revealed a giant liver hemangioma (7x13 cm in right lobe of the liver with signals of recent bleeding. Large feeding artery was embolised to reduce bleeding risk. The surgery occurred without transfusion. The liver trans-section was done with 75 mm linear stapler. The patient was discharged in the 13th post-operative day. CONCLUSION: Surgery is mandatory in hepatic hemangioma rupture and embolization is efficient in controlling pre-operative bleeding giving better opportunity to successful surgical procedure.

  19. arXiv Machine learning challenges in theoretical HEP

    CERN Document Server

    Carrazza, Stefano

    2017-01-01

    In these proceedings we perform a brief review of machine learning (ML) applications in theoretical High Energy Physics (HEP-TH). We start the discussion by defining and then classifying machine learning tasks in theoretical HEP. We then discuss some of the most popular and recent published approaches with focus on a relevant case study topic: the determination of parton distribution functions (PDFs) and related tools. Finally, we provide an outlook about future applications and developments due to the synergy between ML and HEP-TH.

  20. Preliminary development of flaw evaluation procedures for delayed hydride cracking initiation under hydride non-ratcheting conditions

    Energy Technology Data Exchange (ETDEWEB)

    Xu, S.; Cui, J.; Kawa, D.; Shek, G.K.; Scarth, D.A. [Kinectrics Inc., Toronto, Ontario (Canada)

    2006-07-01

    The flaw evaluation procedure for Delayed Hydride Cracking (DHC) initiation currently provided in the CSA Standard N285.8 was developed for hydride ratcheting conditions, in which flaw-tip hydrides do not completely dissolve at peak temperature. Test results have shown that hydrided regions formed under non-ratcheting conditions, in which flaw-tip hydrides completely dissolve at peak temperature, have significantly higher resistance to cracking than those formed under ratcheting conditions. This paper presents some preliminary work on the development of a procedure for the evaluation of DHC initiation for flaws under hydride non-ratcheting conditions. (author)

  1. Inflammation response at the transcriptional level of HepG2 cells induced by multi-walled carbon nanotubes

    Energy Technology Data Exchange (ETDEWEB)

    Piret, Jean-Pascal; Vankoningsloo, Sebastien; Noel, Florence; Saout, Christelle; Toussaint, Olivier [Research Unit in Cellular Biology (URBC), Narilis, University of Namur, 5000 Namur (Belgium); Mendoza, Jorge Mejia; Lucas, Stephane, E-mail: olivier.toussaint@fundp.ac.be [Research Center for the Physics of Matter and Radiation (PMR), Narilis, University of Namur, 5000 Namur (Belgium)

    2011-07-06

    Poor information are currently available about the biological effects of multi-walled carbon nanotubes (MWCNT) on the liver. In this study, we evaluated the effects of MWCNT at the transcriptional level on the classical in vitro model of HepG2 hepatocarcinoma cells. The expression levels of 96 transcript species implicated in the inflammatory and immune responses was studied after a 24h incubation of HepG2 cells in presence of raw MWCNT dispersed in water by stirring. Among the 46 transcript species detected, only a few transcripts including mRNA coding for interleukine-7, chemokines receptor of the C-C families CCR7, as well as Endothelin-1, were statistically more abundant after treatment with MWCNT. Altogether, these data indicate that MWCNT can only induce a weak inflammatory response in HepG2 cells.

  2. Inflammation response at the transcriptional level of HepG2 cells induced by multi-walled carbon nanotubes

    Science.gov (United States)

    Piret, Jean-Pascal; Vankoningsloo, Sébastien; Noël, Florence; Mejia Mendoza, Jorge; Lucas, Stéphane; Saout, Christelle; Toussaint, Olivier

    2011-07-01

    Poor information are currently available about the biological effects of multi-walled carbon nanotubes (MWCNT) on the liver. In this study, we evaluated the effects of MWCNT at the transcriptional level on the classical in vitro model of HepG2 hepatocarcinoma cells. The expression levels of 96 transcript species implicated in the inflammatory and immune responses was studied after a 24h incubation of HepG2 cells in presence of raw MWCNT dispersed in water by stirring. Among the 46 transcript species detected, only a few transcripts including mRNA coding for interleukine-7, chemokines receptor of the C-C families CCR7, as well as Endothelin-1, were statistically more abundant after treatment with MWCNT. Altogether, these data indicate that MWCNT can only induce a weak inflammatory response in HepG2 cells.

  3. Evaluation of enamel surface after orthodontic debonding and cleanup using different procedures: An in vitro study

    Directory of Open Access Journals (Sweden)

    Harjoy Khatria

    2016-01-01

    Full Text Available Objectives: The purpose of this in vitro study was to evaluate and compare the efficiency of four protocols of adhesive remnant removal and polishing after bracket debonding on enamel surfaces using scanning electron microscope (SEM and to compare the time spent to remove resin remnants. Materials and Methods: The present study was a comparative study, in which brackets were bonded on forty freshly extracted human premolar teeth. They were debonded after 24 h and removal of remnant adhesive to as close as possible to the original condition using tungsten carbide burs (TCBs (30 flutted with low-speed contra-angle handpiece, Super Snap ® discs (course, medium, fine, and superfine, TCB + Brownie and Greenie (BG polishers, and TCB + Super Snap ® discs. The surfaces were evaluated under SEM and graded according to the modified surface roughness index. Time taken to remove the residual adhesive was recorded using a stopwatch. Results were subjected to statistical analysis. Results: Super Snap ® discs showed a smooth surface with minimal scratches. TCB resulted in an irregular enamel surface in SEM evaluation, showing horizontal scars with a consistent pattern and left remnants on the enamel surfaces. TCB followed by Super Snap ® discs produced some scratches on the enamel surface. The mean time was significantly higher in Group I than the other three groups (P = 0.000. The mean time was significantly lower in Group II than the other three groups (P = 0.000. TCB took the least amount of time followed by TCB + stainless steel and TCB + BG polishers. Conclusion: Enamel surface was restored as close to the original using the Super Snap ® discs. TCB produced a very rough surface, but it is an efficient and least time-consuming procedure. The resultant enamel surface with enamel scars needs to be finished by other polishing techniques after bulk removal using TCB as the sequential use of Super Snap ® discs and polishers is less aggressive in removing

  4. Procedural Audio in Computer Games Using Motion Controllers: An Evaluation on the Effect and Perception

    Directory of Open Access Journals (Sweden)

    Niels Böttcher

    2013-01-01

    Full Text Available A study has been conducted into whether the use of procedural audio affects players in computer games using motion controllers. It was investigated whether or not (1 players perceive a difference between detailed and interactive procedural audio and prerecorded audio, (2 the use of procedural audio affects their motor-behavior, and (3 procedural audio affects their perception of control. Three experimental surveys were devised, two consisting of game sessions and the third consisting of watching videos of gameplay. A skiing game controlled by a Nintendo Wii balance board and a sword-fighting game controlled by a Wii remote were implemented with two versions of sound, one sample based and the other procedural based. The procedural models were designed using a perceptual approach and by alternative combinations of well-known synthesis techniques. The experimental results showed that, when being actively involved in playing or purely observing a video recording of a game, the majority of participants did not notice any difference in sound. Additionally, it was not possible to show that the use of procedural audio caused any consistent change in the motor behavior. In the skiing experiment, a portion of players perceived the control of the procedural version as being more sensitive.

  5. Evaluation of radiation dose to pediatric patients during certain special procedures

    Science.gov (United States)

    Sulieman, A.; Alzimami, K.; Elhag, B.; Babikir, E.; Alsafi, K.

    2014-11-01

    This study was intended to measure pediatric entrance surface air kerma (ESAK) and effective dose during micturating cystourethrography (MCU), intravenous urography (IVU) and barium studies (barium meal, enema, and swallow) and to propose a local diagnostic reference level (DRL). ESAK was measured for patients using calibrated thermoluminescent dosimeters (TLDs, GR200A). Effective doses (E) were calculated using the National Radiological Protection Board (NRPB) software. A total of 236 special pediatric procedures were investigated. 21.7% of the sample comprised barium procedures, 18.6% were MCU procedures while 59.5% of the sample were IVU procedures. The mean ESAK measurements (mGy) were 2.1±0.8, 3.0±23 and 1.2±0.2 for barium meal, enema and swallow in the same order. The mean patient dose for IVU procedures was 12.4±8.7 mGy per procedure and the mean patient dose per MCU procedure was 5.8±7 mGy. Local DRLs were proposed for all procedures. The patient doses in this study are within the reported values, suggesting that pediatric patients are adequately protected.

  6. Proposal for an Evaluation Method for the Performance of Work Procedures

    Directory of Open Access Journals (Sweden)

    Mouda Mohammed

    2016-12-01

    Conclusion: The proposed method emphasizes to put in value the inputs of continuous improvement of the work procedures in relation with the conventional approaches which adopt the obvious evidence of the noncompliance to the working procedures and seek to analyze the cause–effect relationships related to this unacceptable phenomenon, especially in strategic industry.

  7. Collect Available Creep-Fatigue Data and Study Existing Creep-Fatigue Evaluation Procedures for Grade 91 and Hastelloy XR

    Energy Technology Data Exchange (ETDEWEB)

    Tai Asayama; Yukio Tachibana

    2007-09-30

    This report describes the results of investigation on Task 5 of DOE/ASME Materials Project based on a contract between ASME Standards Technology, LLC (ASME ST-LLC) and Japan Atomic Energy Agency (JAEA). Task 5 is to collect available creep-fatigue data and study existing creep-fatigue evaluation procedures for Grade 91 steel and Hastelloy XR. Part I of this report is devoted to Grade 91 steel. Existing creep-fatigue data were collected (Appendix A) and analyzed from the viewpoints of establishing a creep-fatigue procedure for VHTR design. A fair amount of creep-fatigue data has been obtained and creep-fatigue phenomena have been clarified to develop design standards mainly for fast breeder reactors. Following this, existing creep-fatigue procedures were studied and it was clarified that the creep-fatigue evaluation procedure of the ASME-NH has a lot of conservatisms and they were analyzed in detail from the viewpoints of the evaluation of creep damage of material. Based on the above studies, suggestions to improve the ASME-NH procedure along with necessary research and development items were presented. Part II of this report is devoted to Hastelloy XR. Existing creep-fatigue data used for development of the high temperature structural design guideline for High Temperature Gas-cooled Reactor (HTGR) were collected. Creep-fatigue evaluation procedure in the design guideline and its application to design of the intermediate heat exchanger (IHX) for High Temperature Engineering Test Reactor (HTTR) was described. Finally, some necessary research and development items in relation to creep-fatigue evaluation for Gen IV and VHTR reactors were presented.

  8. Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2.

    Science.gov (United States)

    Schwarzlin, Romina; Pušenjak, Nika; Makuc, Damjan; Križman, Mitja; Vovk, Irena; Plavec, Janez; Švajger, Urban

    2016-10-18

    It had been demonstrated that sugars from various plants can act as potent agents, which induce apoptosis of cancer cells. Using HPLC, we fractionated a mixture of two plant extracts from the plant family Solanaceae, namely Capsicum chinense and the plant family Amaryllidaceae namely Allium sativum. We evaluated the effect of different fractions on apoptosis of HepG2 cell line. The most effective fraction was further studied to determine its molecular composition using mass spectrometry (MS) and NMR. We further evaluated the effect of determined molecular composition found in the selected fraction by using a mixture of commercially available substances, which were found in the fraction and tested its pro-apoptotic effect on HepG2 cells. To get some insight into potential apoptotic mechanisms we studied caspase-3 activity and mitochondrial integrity in treated cells. Out of 93 fractions obtained by HPLC from the plant extract we found HPLC fraction 10 (10 min elution) was the most effective. MS and NMR studies revealed high presence of cellobiose together with vitamin C, sulphur (S) and trace amounts of selenium (Se). HPLC fraction 10 triggered apoptosis of HepG2 within 3 h in the 0.01-1.0 mg/mL concentration range. Furthermore, a mixture of pure cellobiose, vitamin C, S and Se (complex cellobiose/C/S/Se) had a very similar capacity in inducing apoptosis of HepG2 cells compared to HPLC fraction 10. Complex cellobiose/C/S/Se was capable of inducing caspase-3 activity and led to loss of mitochondrial integrity. The capacity of cellobiose alone to induce apoptosis of HepG2 was approximately 1000-fold lower compared to complex cellobiose/C/S/Se. In this study we present the highly synergistic effect of a unique complex consisting of cellobiose, vitamin C, sulphur and selenium on triggering the apoptosis of human hepatocellular carcinoma (HepG2) cell line.

  9. Cytotoxicity and apoptotic effects of tea polyphenol-loaded chitosan nanoparticles on human hepatoma HepG2 cells

    Energy Technology Data Exchange (ETDEWEB)

    Liang, Jin [Key Laboratory of Tea Biochemistry and Biotechnology of Ministry of Education and Ministry of Agriculture, Anhui Agricultural University, Hefei 230036 (China); College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); Li, Feng [College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); Fang, Yong; Yang, Wenjian [College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023 (China); An, Xinxin; Zhao, Liyan; Xin, Zhihong; Cao, Lin [College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); Hu, Qiuhui, E-mail: qiuhuihu@njau.edu.cn [College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023 (China)

    2014-03-01

    Tea polyphenols have strong antioxidant and antitumor activities. However, these health benefits are limited due to their poor in vivo stability and low bioavailability. Chitosan nanoparticles as delivery systems may provide an alternative approach for enhancing bioavailability of poorly absorbed drugs. In this study, tea polyphenol-loaded chitosan nanoparticles have been prepared using two different chitosan biomaterials, and their antitumor effects were evaluated in HepG2 cells, including cell cytotoxicity comparison, cell morphology analysis, cell apoptosis and cell cycle detection. The results indicated that the tea polyphenol-loaded chitosan nanoparticles showed a branch shape and heterogeneous distribution in prepared suspension. MTT assay suggested that tea polyphenol-loaded chitosan nanoparticles could inhibit the proliferation of HepG2 cells, and the cytotoxicity rates were increased gradually and appeared an obvious dose-dependent relationship. Transmission electron microscope images showed that the HepG2 cells treated with tea polyphenol-loaded chitosan nanoparticles exhibited some typical apoptotic features, such as microvilli disappearance, margination of nuclear chromatin, intracytoplasmic vacuoles and the mitochondrial swelling. In addition, the tea polyphenol-loaded chitosan nanoparticles had relatively weak inhibitory effects on HepG2 cancer cells compared with tea polyphenols. Tea polyphenols not only induced cancer cell apoptosis, but also promoted their necrosis. However, tea polyphenol-loaded chitosan nanoparticles exhibited their antitumor effects mainly through inducing cell apoptosis. Our results revealed that the inhibition effects of tea polyphenol-loaded chitosan nanoparticles on tumor cells probably depended on their controlled drug release and effective cell delivery. The chitosan nanoparticles themselves as the delivery carrier showed limited antitumor effects compared with their encapsulated drugs. - Highlights: • Tea polyphenol

  10. UARS PEM Level 2 HEPS B V001

    Data.gov (United States)

    National Aeronautics and Space Administration — The Particle Environment Monitor (PEM) level 2 High-Energy Particle Spectrometer (HEPS) B daily product contains the electron high-resolution spectral data converted...

  11. UARS PEM Level 2 HEPS A V001

    Data.gov (United States)

    National Aeronautics and Space Administration — The Particle Environment Monitor (PEM) level 2 High-Energy Particle Spectrometer (HEPS) A daily product contains electron high-resolution spectral data converted to...

  12. Biosynthesis of hematite nanoparticles and its cytotoxic effect on HepG2 cancer cells.

    Science.gov (United States)

    Rajendran, Kumar; Karunagaran, Vithiya; Mahanty, Biswanath; Sen, Shampa

    2015-03-01

    Iron oxide nanoparticles were gaining significant importance in a variety of applications due to its paramagnetic properties and biocompatibility. Various chemical methods were employed for hematite nanoparticle synthesis which require special equipment or a complex production process. In this study, protein capped crystalline hexagonal hematite (α-Fe2O3) nanoparticles were synthesized by green approach using culture supernatant of a newly isolated bacterium, Bacillus cereus SVK1 at ambient conditions. The synthesized nanoparticles were characterized by electron microscopy, X-ray diffraction, UV-visible spectroscopy and Fourier transform infrared spectroscopic analysis. Nanoparticles were evaluated for its possible anticancer activity against HepG2 liver cancer cells by MTT assay. Hematite nanoparticles with an average diameter of 30.2 nm, exhibited a significant cytotoxicity toward HepG2 cells in a concentration-dependent manner (CTC50=704 ng/ml). Copyright © 2014 Elsevier B.V. All rights reserved.

  13. Mapping modern software process engineering techniques onto an HEP development environment

    CERN Document Server

    Wellisch, J P

    2003-01-01

    One of the most challenging issues faced in HEP in recent years is the question of how to capitalise on software development and maintenance experience in a continuous manner. To capitalise means in our context to evaluate and apply new process technologies as they arise, and to further evolve technologies already widely in use. It also implies the definition and adoption of standards. The CMS off- line software improvement effort aims at continual software quality improvement, and continual improvement in the efficiency of the working environment with the goal to facilitate doing great new physics. To achieve this, we followed a process improvement program based on ISO-15504, and Rational Unified Process. This experiment in software process improvement in HEP has been progressing now for a period of 3 years. Taking previous experience from ATLAS and SPIDER into account, we used a soft approach of continuous change within the limits of current culture to create of de facto software process standards within th...

  14. Clinical and Radiographic Evaluation of Procedural Accidents and Errors during Root Canal Therapy.

    Science.gov (United States)

    Mozayeni, Mohammad Ali; Asnaashari, Mohammad; Modaresi, Seyed Jalil

    2006-01-01

    Root canal therapy (RCT)_like other dental practices_ can be accompanied with some accidents or unpredictable conditions that are called "procedural accidents". Having the knowledge about these accidents and their etiology is essential to have RCT completion and to prevent the repeat of these accidents. This study was designed to evaluate accidents occurring during RCT in patients referred to endodontic department of Shaheed Beheshti dental school during 2002. This study was conducted via descriptive method. Data were collected from observation, clinical examination and oral radiographs, and were recorded in questionnaires, 150 cases from the patients referred to endodontic department were selected randomly and different observed RCT errors were analyzed by SPSS software. The Chi-square and Fisher exact tests were used for analysis. The study showed that 101 patients (67.3%) had one type of RCT errors, and remaining (32.7%) were error free. From the errors studied the most prevalent were "void" which was observed in 41 patients (27.3%), and "ledge" in 39 patients (26%) respectively. The prevalence of other accidents were underfilling in 35 patients (23.3%), poor shaping in 30 patients (20%), overfilling in 23 patients (15.3%), transportation in 13 patients (8.7%), zipping in 3 patients (2%), gouging in 1 patients (0.7%), and strip perforation in 1 patients (0.7%), while no case of broken instruments, vertical fracture, furcation and cervical perforation was observed. The most prevalent errors were found in instrumentation and obturation steps, therefore more care and attention must be paid to instructor observation and the education of these stages.

  15. Novel test procedure to evaluate the treatability of wastewater with ozone.

    Science.gov (United States)

    Schindler Wildhaber, Yael; Mestankova, Hana; Schärer, Michael; Schirmer, Kristin; Salhi, Elisabeth; von Gunten, Urs

    2015-05-15

    Organic micropollutants such as pharmaceuticals, estrogens or pesticides enter the environment continuously through the effluent of municipal wastewater treatment plants (WWTPs). Enhanced treatment of wastewater (WW) by ozone (O3) is probably one of the simplest measures for abatement of organic micropollutants to avoid their discharge to the aquatic environment. During ozonation most organic micropollutants present in treated WW are oxidized either by a direct reaction with O3 or by secondarily formed hydroxyl radicals (OH). However, undesired oxidation by-products from the oxidative transformation of matrix components can also be formed. A modular laboratory decision tool based on the findings of previous investigations is presented to test the feasibility of ozonation as an option to upgrade specific WWTPs. These modules consist of investigations to assess (i) the matrix effects on ozone stability, (ii) the efficiency of micropollutant removal, (iii) the oxidation by-product formation, as well as (iv) bioassays to measure specific and unspecific toxicity of the treated WWs. Matrix effects on ozone stability (quantified as O3 and OH exposures) can give first indications on the suitability of an ozonation step. Ozonation of WWs yielding O3 and OH exposures and micropollutant abatement similar to reference values evoked a significant improvement of the water quality as indicated by a broad range of bioassays. Irregular behavior of the ozonation points towards unknown compounds, possibly leading to the formation of undesired degradation products. It has been observed that in such WWs ozonation partly enhanced toxicity. In summary, the presented tiered laboratory test procedure represents a relatively cheap and straight-forward methodology to evaluate the feasibility of ozonation to upgrade specific WWTPs for micropollutant removal based on chemical and biological measurements. Copyright © 2015 Elsevier Ltd. All rights reserved.

  16. Evaluation of some procedures relevant to the determination of trace elemental components in biological materials by destructive neutron activation analysis

    Energy Technology Data Exchange (ETDEWEB)

    Berry, D.L.

    1979-01-01

    The development of a simplified procedure for the analysis of biological materials by destructive neutron activation analysis (DNAA) is described. The sample manipulations preceding gamma ray assay were investigated as five specific stages of processing: (1) pre-irradiation treatment; (2) sample irradiation; (3) removal of the organic matrix; (4) removal of interfering radioactivities; and (5) concentration and separation of analyte activities. Each stage was evaluated with respect to susceptibility to sample contamination, loss of trace elemental components, and compatibility with other operations in the overall DNAA procedures. A complete DNAA procedure was proposed and evaluated for the analysis of standard bovine liver and blood samples. The DNAA system was effective for the determination of As, Cu, Fe, Hg, Mo, Rb, Sb, Se, and Zn without yield determinations and with a minimum turn-around time of approximately 3 days.

  17. Utilidad de la elastografía de transición (Fibroscan® en la evaluación de la fibrosis hepática en pacientes con hepatopatía crónica Usefulness of transient elastography (Fibroscan® in the assessment of fibrosis in patients with chronic liver disease

    Directory of Open Access Journals (Sweden)

    Daniel Álvarez

    2012-02-01

    Full Text Available El pronóstico de la enfermedad crónica hepática depende de la extensión y la progresión de la fibrosis hepática. Actualmente la biopsia hepática es la técnica de elección para determinar el grado de fibrosis, pero es una prueba invasiva, no exenta de complicaciones. Por ello, el desarrollo de marcadores no invasivos de fibrosis hepática se convirtió en una necesidad indiscutible. Se propuso la elastografìa por transición (Fibroscan® para valorar la fibrosis hepática en pacientes con enfermedad crónica hepática, mediante la medición de la rigidez hepática. Nuestro objetivo fue evaluar la efectividad, la objetividad y la seguridad de esta técnica. Se estudiaron 68 pacientes a los que se les realizó una biopsia hepática en los 18 meses previos al estudio. Todos los procedimientos de elastografia y biopsia hepática fueron analizados por un mismo profesional (DA y MA, respectivamente. Para la valoración de la biopsia hepática se utilizó la escala METAVIR. El valor medio de rigidez en pacientes sin fibrosis o con fibrosis leve (F0-F1 y en los pacientes con fibrosis avanzada o cirrosis (F3-F4 fue 6.8 ± 3.0 kPa y 21.0 ± 15.1 kPa, respectivamente (con diferencia significativa, p The prognosis and management of chronic liver disease largely depends on the extent and progression of liver fibrosis. Unfortunately, liver biopsy, an invasive and painful technique with several limitations, continues to be the gold standard for the staging and grading of fibrosis. Therefore, accurate noninvasive tests for liver injury are urgently needed. During the last years, transient elastography (Fibroscan® has been proposed for the assessment of hepatic fibrosis in patients with chronic liver disease, by measuring liver stiffness. The aim of this study was to evaluate the effectiveness, objectivity and safety of this technique. We included 68 patients who underwent a liver biopsy in the last 18 months with a wide spectrum of chronic liver

  18. Computer-based procedure for field activities: Results from three evaluations at nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Oxstrand, Johanna [Idaho National Lab. (INL), Idaho Falls, ID (United States); bly, Aaron [Idaho National Lab. (INL), Idaho Falls, ID (United States); LeBlanc, Katya [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2014-09-01

    Nearly all activities that involve human interaction with the systems of a nuclear power plant are guided by procedures. The paper-based procedures (PBPs) currently used by industry have a demonstrated history of ensuring safety; however, improving procedure use could yield tremendous savings in increased efficiency and safety. One potential way to improve procedure-based activities is through the use of computer-based procedures (CBPs). Computer-based procedures provide the opportunity to incorporate context driven job aids, such as drawings, photos, just-in-time training, etc into CBP system. One obvious advantage of this capability is reducing the time spent tracking down the applicable documentation. Additionally, human performance tools can be integrated in the CBP system in such way that helps the worker focus on the task rather than the tools. Some tools can be completely incorporated into the CBP system, such as pre-job briefs, placekeeping, correct component verification, and peer checks. Other tools can be partly integrated in a fashion that reduces the time and labor required, such as concurrent and independent verification. Another benefit of CBPs compared to PBPs is dynamic procedure presentation. PBPs are static documents which limits the degree to which the information presented can be tailored to the task and conditions when the procedure is executed. The CBP system could be configured to display only the relevant steps based on operating mode, plant status, and the task at hand. A dynamic presentation of the procedure (also known as context-sensitive procedures) will guide the user down the path of relevant steps based on the current conditions. This feature will reduce the user’s workload and inherently reduce the risk of incorrectly marking a step as not applicable and the risk of incorrectly performing a step that should be marked as not applicable. As part of the Department of Energy’s (DOE) Light Water Reactors Sustainability Program

  19. 9 CFR 147.16 - Procedure for the evaluation of mycoplasma reactors by in vivo bio-assay (enrichment).

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Procedure for the evaluation of mycoplasma reactors by in vivo bio-assay (enrichment). 147.16 Section 147.16 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT AUXILIARY...

  20. Influence of handling procedures and biological factors on the QIM evaluation of whole herring (Clupea harengus L.)

    DEFF Research Database (Denmark)

    Nielsen, Durita; Hyldig, Grethe

    2004-01-01

    QIM evaluations were performed on herring from ten seasonally and geographically distributed cruises and related to handling procedures and biological and chemical parameters. The results showed clear effects from onboard storage methods. The quality of iced herring was superior to the quality...

  1. Study of a Docker Use-Case for HEP

    Energy Technology Data Exchange (ETDEWEB)

    Kowalkowski, Jim [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Lyon, Adam [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Paterno, Marc [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)

    2016-01-01

    Over the past few years, container technology has become increasingly promising as a means to seamlessly make our software available across a wider range of platforms. In December 2015, we decided to put together a set of docker images that serve as a demonstration of this container technology for managing a run-time environment for art-related software projects, and also serve as a set of test cases for evaluation of performance. Docker[1] containers provide a way to “wrap up a piece of software in a complete filesystem that contains everything it needs to run”. In combination with Shifter[2], such containers provide a way to run software developed and deployed on “typical” HEP platforms (such as SLF 6, in common use at Fermilab and on OSG platforms) on HPC facilities at NERSC. Docker containers provide a means of delivering software that can be run on a variety of hosts without needing to be compiled specially for each OS to be supported. This could substantially reduce the effort required to create and validate a new release, since one build could be suitable for use on both grid machines (both FermiGrid and OSG) as well as any machine capable of running the Docker container. In addition, docker containers may provide for a quick and easy way for users to install and use a software release in a standardized environment. This report contains the results and status of this demonstration and evaluation.

  2. Identifying Memory Allocation Patterns in HEP Software

    Science.gov (United States)

    Kama, S.; Rauschmayr, N.

    2017-10-01

    HEP applications perform an excessive amount of allocations/deallocations within short time intervals which results in memory churn, poor locality and performance degradation. These issues are already known for a decade, but due to the complexity of software frameworks and billions of allocations for a single job, up until recently no efficient mechanism has been available to correlate these issues with source code lines. However, with the advent of the Big Data era, many tools and platforms are now available to do large scale memory profiling. This paper presents, a prototype program developed to track and identify each single (de-)allocation. The CERN IT Hadoop cluster is used to compute memory key metrics, like locality, variation, lifetime and density of allocations. The prototype further provides a web based visualization back-end that allows the user to explore the results generated on the Hadoop cluster. Plotting these metrics for every single allocation over time gives a new insight into application’s memory handling. For instance, it shows which algorithms cause which kind of memory allocation patterns, which function flow causes how many short-lived objects, what are the most commonly allocated sizes etc. The paper will give an insight into the prototype and will show profiling examples for the LHC reconstruction, digitization and simulation jobs.

  3. Evaluation of endoscopic laser excision of polypropylene mesh/sutures following anti-incontinence procedures.

    LENUS (Irish Health Repository)

    Davis, N F

    2012-11-01

    We reviewed our experience with and outcome of the largest series to our knowledge of patients who underwent endoscopic laser excision of eroded polypropylene mesh or sutures as a complication of previous anti-incontinence procedures.

  4. Evaluation of the ROSA™ Spine robot for minimally invasive surgical procedures.

    Science.gov (United States)

    Lefranc, M; Peltier, J

    2016-10-01

    The ROSA® robot (Medtech, Montpellier, France) is a new medical device designed to assist the surgeon during minimally invasive spine procedures. The device comprises a patient-side cart (bearing the robotic arm and a workstation) and an optical navigation camera. The ROSA® Spine robot enables accurate pedicle screw placement. Thanks to its robotic arm and navigation abilities, the robot monitors movements of the spine throughout the entire surgical procedure and thus enables accurate, safe arthrodesis for the treatment of degenerative lumbar disc diseases, exactly as planned by the surgeon. Development perspectives include (i) assistance at all levels of the spine, (ii) improved planning abilities (virtualization of the entire surgical procedure) and (iii) use for almost any percutaneous spinal procedures not limited in screw positioning such as percutaneous endoscopic lumbar discectomy, intracorporeal implant positioning, over te top laminectomy or radiofrequency ablation.

  5. Biópsia hepática por laparotomia paracostal em bovinos e búfalos Paracostal liver biopsy in cattle and buffalo

    Directory of Open Access Journals (Sweden)

    Antonio Humberto Hamad Minervino

    2009-06-01

    Full Text Available A técnica de biópsia hepática em ruminantes tem importante valor no diagnóstico clínico de doenças tóxicas e metabólicas, em especial nos desequilíbrios minerais. As técnicas mais comumente utilizadas restringem análises devido ao limitado volume de tecido obtido. No presente trabalho, avaliou-se o uso de uma técnica de biópsia hepática por laparotomia paracostal em bovinos e búfalos. Foram utilizados 10 bovinos e 10 búfalos hígidos. Os animais foram mantidos em estação, sedados com xilazina e infiltrados localmente com lidocaína e epinefrina. O acesso à cavidade abdominal foi realizado por meio de uma incisão dorso-ventral de 15cm no flanco direito, iniciada ventralmente (cerca de 4-5cm ao processo transverso da 2a ou 3a vértebra lombar e situada caudalmente (cerca de 4cm e paralelamente à 13a costela, obtendo-se visualização do fígado. Foi então realizado pinçamento do bordo caudal do órgão com pinça Doyen para remoção de fragmento hepático (2 a 4g. Procedeu-se o fechamento da cavidade abdominal como de rotina. Foram analisados os parâmetros bioquímicos e hematológicos antes do procedimento (tempo zero e após 24 horas, 48 horas, 5 dias e 10 dias após a biópsia. Todas as variáveis bioquímicas estudadas retornaram aos valores basais 5 e 10 dias após o procedimento nos bovinos e búfalos, respectivamente. O tempo médio de cirurgia por animal foi de 25 minutos. A biópsia hepática por laparotomia paracostal demonstrou ser uma técnica eficaz e de baixo risco à saúde dos animais, permitindo a coleta de suficiente quantidade de tecido hepática para realização de múltiplas análises.Liver biopsy in ruminants is an important technique for clinical diagnosis of toxic and metabolic diseases, especially mineral disorders. The most frequent procedures used so far results in an small amount of liver and not enough for multiple tests. The present study aims to evaluate the efficacy of paracostal laparotomy

  6. Radiation dose of nurses during IR procedures: a controlled trial evaluating operator alerts before nursing tasks.

    Science.gov (United States)

    Komemushi, Atsushi; Suzuki, Satoshi; Sano, Akira; Kanno, Shohei; Kariya, Shuji; Nakatani, Miyuki; Yoshida, Rie; Kono, Yumiko; Ikeda, Koshi; Utsunomiya, Keita; Harima, Yoko; Komemushi, Sadao; Tanigawa, Noboru

    2014-08-01

    To compare radiation exposure of nurses when performing nursing tasks associated with interventional procedures depending on whether or not the nurses called out to the operator before approaching the patient. In a prospective study, 93 interventional radiology procedures were randomly divided into a call group and a no-call group; there were 50 procedures in the call group and 43 procedures in the no-call group. Two monitoring badges were used to calculate effective dose of nurses. In the call group, the nurse first told the operator she was going to approach the patient each time she was about to do so. In the no-call group, the nurse did not say anything to the operator when she was about to approach the patient. In all the nursing tasks, the equivalent dose at the umbilical level inside the lead apron was below the detectable limit. The equivalent dose at the sternal level outside the lead apron was 0.16 μSv ± 0.41 per procedure in the call group and 0.51 μSv ± 1.17 per procedure in the no-call group. The effective dose was 0.018 μSv ± 0.04 per procedure in the call group and 0.056 μSv ± 0.129 per procedure in the no-call group. The call group had a significantly lower radiation dose (P = .034). Radiation doses of nurses were lower in the group in which the nurse called to the operator before she approached the patient. Copyright © 2014 SIR. Published by Elsevier Inc. All rights reserved.

  7. Performance evaluation of different types of particle representation procedures of Particle Swarm Optimization in Job-shop Scheduling Problems

    Science.gov (United States)

    Izah Anuar, Nurul; Saptari, Adi

    2016-02-01

    This paper addresses the types of particle representation (encoding) procedures in a population-based stochastic optimization technique in solving scheduling problems known in the job-shop manufacturing environment. It intends to evaluate and compare the performance of different particle representation procedures in Particle Swarm Optimization (PSO) in the case of solving Job-shop Scheduling Problems (JSP). Particle representation procedures refer to the mapping between the particle position in PSO and the scheduling solution in JSP. It is an important step to be carried out so that each particle in PSO can represent a schedule in JSP. Three procedures such as Operation and Particle Position Sequence (OPPS), random keys representation and random-key encoding scheme are used in this study. These procedures have been tested on FT06 and FT10 benchmark problems available in the OR-Library, where the objective function is to minimize the makespan by the use of MATLAB software. Based on the experimental results, it is discovered that OPPS gives the best performance in solving both benchmark problems. The contribution of this paper is the fact that it demonstrates to the practitioners involved in complex scheduling problems that different particle representation procedures can have significant effects on the performance of PSO in solving JSP.

  8. Antiproliferative activity and phenotypic modification induced by selected Peruvian medicinal plants on human hepatocellular carcinoma Hep3B cells.

    Science.gov (United States)

    Carraz, Maëlle; Lavergne, Cédric; Jullian, Valérie; Wright, Michel; Gairin, Jean Edouard; Gonzales de la Cruz, Mercedes; Bourdy, Geneviève

    2015-05-26

    The high incidence of human hepatocellular carcinoma (HCC) in Peru and the wide use of medicinal plants in this country led us to study the activity against HCC cells in vitro of somes species used locally against liver and digestive disorders. Ethnopharmacological survey: Medicinal plant species with a strong convergence of use for liver and digestive diseases were collected fresh in the wild or on markets, in two places of Peru: Chiclayo (Lambayeque department, Chiclayo province) and Huaraz (Ancash department, Huaraz province). Altogether 51 species were collected and 61 ethanol extracts were prepared to be tested. Biological assessment: All extracts were first assessed against the HCC cell line Hep3B according a 3-step multi-parametric phenotypic assay. It included 1) the evaluation of phenotypic changes on cells by light microscopy, 2) the measurement of the antiproliferative activity and 3) the analysis of the cytoskeleton and mitosis by immunofluorescence. Best extracts were further assessed against other HCC cell lines HepG2, PLC/PRF/5 and SNU-182 and their toxicity measured in vitro on primary human hepatocytes. Ethnopharmacological survey: Some of the species collected had a high reputation spreading over the surveyed locations for treating liver problems, i.e. Baccharis genistelloides, Bejaria aestuans, Centaurium pulchellum, Desmodium molliculum, Dipsacus fullonum, Equisetum bogotense, Gentianella spp., Krameria lapacea, Otholobium spp., Schkuhria pinnata, Taraxacum officinale. Hep3B evaluation: Fourteen extracts from 13 species (Achyrocline alata, Ambrosia arborescens, Baccharis latifolia, Hypericum laricifolium, Krameria lappacea, Niphidium crassifolium, Ophryosporus chilca, Orthrosanthus chimboracensis, Otholobium pubescens, Passiflora ligularis, Perezia coerulescens, Perezia multiflora and Schkuhria pinnata) showed a significant antiproliferative activity against Hep3B cells (IC50≤ 50µg/mL). This was associated with a lack of toxicity on primary

  9. Evaluation of sedation for standing clinical procedures in horses using detomidine combined with buprenorphine.

    Science.gov (United States)

    Taylor, Polly; Coumbe, Karen; Henson, Frances; Scott, David; Taylor, Alan

    2014-01-01

    To examine the effect of including buprenorphine with detomidine for sedation of horses undergoing clinical procedures. Partially blinded, randomised, prospective clinical field trial. Eighty four client-owned horses scheduled for minor surgery or diagnostic investigation under standing sedation. The effects of buprenorphine (5 μg kg(-1) ) (Group B, n = 46) or placebo (5% glucose solution) (Group C, n = 38) in combination with detomidine (10 μg kg(-1) ) were compared in standing horses undergoing minor clinical procedures. The primary outcome measure was successful completion of the procedure. The degree of sedation and ataxia were scored using simple descriptive scales. Heart and respiratory rates were recorded at 15-30 minute intervals. Parametric data from each group were compared using anova or t-test and non parametric data using the Mann-Whitney U test. The procedure was carried out successfully in 91% of Group B and 63% of Group C (p buprenorphine but not glucose administration, was more profound in group B and lasted longer (60 versus 30 minutes) p Buprenorphine 5 and 10 μg kg(-1) enhanced the sedation produced by detomidine 10 and 20 μg kg(-1) with minor side effects similar to other alpha2 agonist/opioid combinations. Detomidine-buprenorphine sedation is suitable for standing procedures in horses. © 2013 Association of Veterinary Anaesthetists and the American College of Veterinary Anesthesia and Analgesia.

  10. Parameter-free effective field theory calculation for the solar proton-fusion and hep processes

    Energy Technology Data Exchange (ETDEWEB)

    T.S. Park; L.E. Marcucci; R. Schiavilla; M. Viviani; A. Kievsky; S. Rosati; K. Kubodera; D.P. Min; M. Rho

    2002-08-01

    Spurred by the recent complete determination of the weak currents in two-nucleon systems up to {Omicron}(Q{sup 3}) in heavy-baryon chiral perturbation theory, we carry out a parameter-free calculation of the threshold S-factors for the solar pp (proton-fusion) and hep processes in an effective field theory that combines the merits of the standard nuclear physics method and systematic chiral expansion. The power of the EFT adopted here is that one can correlate in a unified formalism the weak-current matrix elements of two-, three- and four-nucleon systems. Using the tritium {beta}-decay rate as an input to fix the only unknown parameter in the theory, we can evaluate the threshold S factors with drastically improved precision; the results are S{sub pp}(0) = 3.94 x (1 {+-} 0.004) x 10{sup -25} MeV-b and S{sub hep}(0) = (8.6 {+-} 1.3) x 10{sup -20} keV-b. The dependence of the calculated S-factors on the momentum cutoff parameter {Lambda} has been examined for a physically reasonable range of {Lambda}. This dependence is found to be extremely small for the pp process, and to be within acceptable levels for the hep process, substantiating the consistency of our calculational scheme.

  11. In Vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2 Cells

    Directory of Open Access Journals (Sweden)

    Michelle C. Búfalo

    2009-01-01

    Full Text Available Propolis is a sticky dark-colored material showing a very complex chemical composition that honeybees collect from plants. It has been used in folk medicine since ancient times, due to several biological properties, such as antimicrobial, anti-inflammatory, antioxidant and immunomodulatory activities, among others. Its antitumor action in vivo and in vitro has also been reported, using propolis extracts or its isolated compounds. The goal of this work was to evaluate propolis's cytotoxic action in vitro on human laryngeal epidermoid carcinoma (Hep-2 cells. These cells were incubated with different concentrations of this bee product for different time periods, and morphology and the number of viable HEp-2 cells analyzed. Data showed that propolis exhibited a cytotoxic effect in vitro against HEp-2 cells, in a dose- and time-dependent way. Propolis solvent had no effects on morphology and number of viable cells, proving that the cytotoxic effects were exclusively due to propolis components. Since humans have been using propolis for a long time, further assays will provide a better comprehension of propolis's antitumor action.

  12. Tripterygium regelii decreases the biosynthesis of triacylglycerol and cholesterol in HepG2 cells.

    Science.gov (United States)

    Kang, Myung-Ji; Kwon, Eun-Bin; Yuk, Heung Joo; Ryu, Hyung Won; Kim, Soo-Yeon; Lee, Mi-Kyeong; Moon, Dong-Oh; Lee, Su Ui; Oh, Sei-Ryang; Lee, Hyun-Sun; Kim, Mun-Ock

    2017-12-01

    In the course of screening to find a plant material decreasing the activity of triacylglycerol and cholesterol, we identified Tripterygium regelii (TR). The methanol extract of TR leaves (TR-LM) was shown to reduce the intracellular lipid contents consisting of triacylglycerol (TG) and cholesterol in HepG2 cells. TR-LM also downregulated the mRNA and protein expression of the lipogenic genes such as SREBP-1 and its target enzymes. Consequently, TR-LM reduced the TG biosynthesis in HepG2 cells. In addition, TR-LM decreased SREBP2 and its target enzyme HMG-CoA reductase, which is involved in cholesterol synthesis. In this study, we evaluated that TR-LM attenuated cellular lipid contents through the suppression of de novo TG and cholesterol biosynthesis in HepG2 cells. All these taken together, TR-LM could be beneficial in regulating lipid metabolism and useful preventing the hyperlipidemia and its complications, in that liver is a crucial tissue for the secretion of serum lipids.

  13. Evaluation of Four Environmental Enrichment Procedures for Coatis (Nasua nasua in Captivity

    Directory of Open Access Journals (Sweden)

    M. R. Pereira

    2013-03-01

    Full Text Available Wild Animal Sorting Centers (WASC keeps animals that were captured from their natural habitat. These animals often suffer stress and may show atypical behavior due the difficulty encountered by them to adapt to captivity. In order to improve the quality of life of these animals was developed this work, in order to study the behavior of coatis subjected to different procedures of environmental enrichment. The study was conducted in WASC of Federal University of Visa, Visa-MG. Data were collected from the observation of two coatis submitted to four different procedures for environmental enrichment. The interaction with the objects of coatis enrichment was effective, stimulating behavioral changes in animals, demonstrating the success of the procedures applied

  14. Effect of small interfering RNA targeting CD47 gene mediated by lentivirus vectors on proliferation and apoptosis of human laryngocarcinoma Hep-2 cells

    Directory of Open Access Journals (Sweden)

    Shu-feng GAO

    2013-09-01

    Full Text Available Objective To study the effect of small interfering RNA(siRNA targeting CD47 gene mediated by lentivirus vectors on proliferation and apoptosis of human laryngocarcinoma Hep-2 cells. Methods After building lentivirus vector, a siRNA directed against CD47 gene was transfected into Hep-2 cells. Cell morphological changes were observed by fluorescence microscopy. The changes in CD47 mRNA expression were detected by semi-quantitative RT-PCR, and the protein of CD47 was evaluated byWestern blotting. Suppression of proliferation and apoptosis of Hep-2 cells were observed by MTT assay. Results After the laryngocarcinoma Hep-2 cells were transfected by CD47-siRNA lentiviral plasmid, it was showed by fluorescence microscopy that the CD47 siRNA was able to effectively suppress the Hep-2 cell proliferation, the cells were deformed and diminished in size obviously, and necrosis and apoptosis were observed. Semi-quantitative RT-PCR andWestern blotting revealed that the expression of CD47 mRNA and protein decreased by 76%–82% and 77%, respectively(P<0.05. Forty-eight hours after the lentivirus vectors transfection, MTT assay revealed that cell apoptosisi ncreased significantly(P<0.01. Conclusion Small interfering RNA targeting CD47 gene mediated by lentivirus vectors can significantly inhibit CD47 gene expression in Hep-2cells and induce cell apoptosis in vitro.

  15. Implications of cost-effectiveness analysis of medical technology. background paper number 5. four common x-ray procedures: problems and prospects for economic evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Wagner, J.L.; Krieger, M.J.

    1982-04-01

    This paper is about the economic evaluation of diagnostic procedures. The issue of economic evaluation is explored in the context of four common diagnostic X-ray procedures: the chest X-ray, the skull X-ray, the barium enema study, and the excretory urogram. The paper is divided into two parts. The first part summarizes the different evaluative models underlying studies of the four diagnostic X-ray procedures and to lay out the strengths and weaknesses of each method. The second part contains four separate chapters summarizing what is known about the utilization, costs, risks, and benefits of each procedure, with particular emphasis on the evaluative methods employed.

  16. Evaluation of patients’ experiences at different stages of the intravitreal injection procedure – what can be improved?

    Directory of Open Access Journals (Sweden)

    Tailor R

    2011-10-01

    Full Text Available Rajen Tailor, Rebecca Beasley, Yit Yang, Niro NarendranWolverhampton and Midland Counties Eye Infirmary, New Cross Hospital, Wolverhampton, UKIntroduction: Intravitreal injection of ranibizumab has become one of the most commonly performed ophthalmic procedures. It is timely to conduct an evaluation of the injection procedure from the patient’s perspective so as to determine ways to improve patient experience. The purpose of this study was to quantitatively describe patients’ experiences of the different stages of the intravitreal injection procedure and provide suggestions for improvement.Method: Following intravitreal injection, patients were administered a questionnaire to score the distress felt for each of ten parts of the whole injection process from the initial waiting to the final instillation of topical antibiotic at the end. A score of higher than 4 was regarded as significantly unpleasant. The proportion of scores above 4 for each step was used to evaluate the relative distress experienced by patients for the different parts of the procedure.Results: A total of 42 patients were surveyed. The step with the highest percentage of patients scoring more than 4 was the injection step (19%. However, cumulatively, the steps relating to the application of the drape, the speculum, and the removal of drape accounted for 53% of scores greater than 4.Conclusion: There is considerable variation in how patients tolerate different stages of the injection procedure. The needle entry was the most unpleasant step followed by the draping steps cumulatively. Use of subconjunctival anesthesia, a perforated drape, and alternative lid exclusion devices may help to improve the patient’s tolerability of the procedure and experience.Keywords: ranibizumab, patient experience, age-related macular degeneration

  17. Light Water Reactor Sustainability Program: Computer-Based Procedures for Field Activities: Results from Three Evaluations at Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Oxstrand, Johanna [Idaho National Lab. (INL), Idaho Falls, ID (United States); Le Blanc, Katya [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bly, Aaron [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2014-09-01

    The Computer-Based Procedure (CBP) research effort is a part of the Light-Water Reactor Sustainability (LWRS) Program, which is a research and development (R&D) program sponsored by Department of Energy (DOE) and performed in close collaboration with industry R&D programs that provides the technical foundations for licensing and managing the long-term, safe, and economical operation of current nuclear power plants. One of the primary missions of the LWRS program is to help the U.S. nuclear industry adopt new technologies and engineering solutions that facilitate the continued safe operation of the plants and extension of the current operating licenses. One area that could yield tremendous savings in increased efficiency and safety is in improving procedure use. Nearly all activities in the nuclear power industry are guided by procedures, which today are printed and executed on paper. This paper-based procedure process has proven to ensure safety; however, there are improvements to be gained. Due to its inherent dynamic nature, a CBP provides the opportunity to incorporate context driven job aids, such as drawings, photos, and just-in-time training. Compared to the static state of paper-based procedures (PBPs), the presentation of information in CBPs can be much more flexible and tailored to the task, actual plant condition, and operation mode. The dynamic presentation of the procedure will guide the user down the path of relevant steps, thus minimizing time spent by the field worker to evaluate plant conditions and decisions related to the applicability of each step. This dynamic presentation of the procedure also minimizes the risk of conducting steps out of order and/or incorrectly assessed applicability of steps.

  18. A screening procedure to evaluate air pollution effects on Class I wilderness areas

    Science.gov (United States)

    Douglas G. Fox; Ann M. Bartuska; James G. Byrne; Ellis Cowling; Richard Fisher; Gene E. Likens; Steven E. Lindberg; Rick A. Linthurst; Jay Messer; Dale S. Nichols

    1989-01-01

    This screening procedure is intended to help wilderness managers conduct "adverse impact determinations" as part of Prevention of Significant Deterioration (PSD) applications for sources that emit air pollutants that might impact Class I wildernesses. The process provides an initial estimate of susceptibility to critical loadings for sulfur, nitrogen, and...

  19. Increasing Seat Belt Use on a College Campus: An Evaluation of Two Prompting Procedures

    Science.gov (United States)

    Clayton, Michael C.; Helms, Bridgett P.

    2009-01-01

    Seat belt use is an important factor in the prevention of automobile accidents involving injuries and fatalities. The current study used a multielement design to compare the "Click It or Ticket" and "Please Buckle Up--I Care" procedures. Results indicate that the Click It or Ticket prompt resulted in a 20-percentage-point increase in seat belt…

  20. Clustering algorithm evaluation and the development of a replacement for procedure 1. [for crop inventories

    Science.gov (United States)

    Lennington, R. K.; Johnson, J. K.

    1979-01-01

    An efficient procedure which clusters data using a completely unsupervised clustering algorithm and then uses labeled pixels to label the resulting clusters or perform a stratified estimate using the clusters as strata is developed. Three clustering algorithms, CLASSY, AMOEBA, and ISOCLS, are compared for efficiency. Three stratified estimation schemes and three labeling schemes are also considered and compared.

  1. The Use of Formative Evaluation Procedures in the Development of a Mathematics Laboratory.

    Science.gov (United States)

    Miller, George R.

    The procedures used in the initial development of a Mathematics Laboratory within the Individually Prescribed Instruction (IPI) Mathematics Program are reported. The thesis of the report is that in the development of educational programs, especially those which are complex, attention should be directed both to the design of the program and the…

  2. Evaluación de la fibrosis hepática en la hepatitis crónica por virus C mediante la aplicación prospectiva del Sabadell's NIHCED score: Sabadell's Non Invasive, Hepatitis C Related-Cirrhosis Early Detection Score Prospective evaluation of liver fibrosis in chronic viral hepatitis C infection using the Sabadell NIHCED: non-invasive hepatitis C related cirrhosis early detection index

    Directory of Open Access Journals (Sweden)

    G. Bejarano

    2009-05-01

    Full Text Available Introducción: la hepatitis crónica por VHC cursa de forma asintomática desarrollando cirrosis hepática y sus complicaciones en un 20-40% de los casos. En estudios previos se ha demostrado que la fibrosis avanzada es un factor pronóstico fundamental. El método gold standard para la valoración del grado de fibrosis es la biopsia hepática. Nuestro grupo ha validado un índice predictivo, el NIHCED (Sabadell's Non Invasive, Hepatitis C related-Cirrosis Early Detection Score, basado en datos demográficos, analíticos y ecográficos para determinar la presencia de cirrosis. Objetivo: nuestro objetivo es el de evaluar si el NIHCED predice la presencia de fibrosis avanzada en los pacientes con hepatitis crónica por virus C. Material y métodos: estudio prospectivo donde se incluyeron pacientes con hepatitis crónica por VHC. Se les realizó una biopsia hepática y el NIHCED. El grado de fibrosis se correlacionó con el valor del NIHCED mediante curva de ROC y el coeficiente de correlación de Spearman. Resultados: se incluyeron un total de 321 pacientes (ratio hombre/mujer 1,27 con una edad media de 48 ± 14 años. La biopsia hepática mostró que 131 (30,5% no tenían fibrosis o era expansión portal, mientras que 190 (69,5% tenían fibrosis avanzada o cirrosis. Para un punto de corte de 6 puntos, la sensibilidad fue del 72%, especificidad del 76,3%, VPP del 81%, VPN del 63,7% y una precisión diagnóstica del 72,5%, con un área bajo la curva fue de 0,787 y un coeficiente de correlación de Spearman de r = 0,65. Conclusiones: el NIHCED predice la presencia de fibrosis avanzada en un elevado porcentaje de pacientes sin necesidad de realizar biopsia hepática.Introduction: liver disease resulting from chronic hepatitis C virus (HCV infection follows an asymptomatic course towards cirrhosis and its complications in 20-40% of cases. Earlier studies demonstrated that advanced fibrosis is a prognostic factor. The "gold standard" for the evaluation

  3. Patient use of social media to evaluate cosmetic treatments and procedures.

    Science.gov (United States)

    Schlichte, Megan J; Karimkhani, Chante; Jones, Trevor; Trikha, Ritika; Dellavalle, Robert P

    2015-04-16

    With a growing sphere of influence in the modern world, online social media serves as a readily accessible interface for communication of information. Aesthetic medicine is one of many industries increasingly influenced by social media, as evidenced by the popular website, "RealSelf," an online community founded in 2006 that compiles ratings, reviews, photographs, and expert physician commentary for nearly 300 cosmetic treatments. To investigate the current preferences of patients regarding cosmetic non-surgical, surgical, and dental treatments on RealSelf and in the documented medical literature. On a single day of data collection, all cosmetic treatments or procedures reviewed on the RealSelf website were tabulated, including name, percent "worth it" rating, total number of reviews, and average cost. Patient satisfaction rates documented in the current medical literature for each cosmetic treatment or procedure were also recorded. Statistical t-testingcomparing RealSelf ratings and satisfaction rates in the literature was performed for each category-non-surgical, surgical, and dental. The top ten most-commonly reviewed non-surgical treatments, top ten most-commonly reviewed surgical procedures, and top 5 most-commonly reviewed dental treatments, along with documented satisfaction rates in the medical literature for each treatment or procedure were recorded in table format and ranked by RealSelf "worth it" rating. Paired t-testing revealed that satisfaction rates documented in the literature were significantly higher than RealSelf "worth it" ratings for both non-surgical cosmetic treatments (p=0.00076) and surgical cosmetic procedures (p=0.00056), with no statistically significant difference for dental treatments. For prospective patients interested in cosmetic treatments or procedures, social media sites such as RealSelf may offer information helpful to decision-making as well enable cosmetic treatment providers to build reputations and expand practices. "Worth

  4. Evaluation of the CCAM Hierarchy and Semi Structured Code for Retrieving Relevant Procedures in a Hospital Case Mix Database

    Science.gov (United States)

    Bousquet, Cédric; Trombert, Béatrice; Souvignet, Julien; Sadou, Eric; Rodrigues, Jean-Marie

    2010-01-01

    In France, clinical procedures are coded with the French procedures classification (Classification Commune des Actes Médicaux, CCAM) and recorded in every hospital. CCAM uses hierarchical semi-structured codes which describe procedures (topography, action, access mode and/or technique). This amount of information could be analyzed and used for clinical and medico-economic evaluation. But relevant and practical data searches are difficult. In this paper we present a use case about searching for endoscopic activities in a case mix database to evaluate the relevance of the hierarchical organization and semi structured codes of CCAM in order to retrieve data already coded using this controlled vocabulary. Precision was 0.79 and recall 0.84 in the hierarchical search whereas precision was 0.94 and recall was 0.81 using part of the code related to access mode and/or technique. We discuss a revision of the CCAM by the use of an existing modeling (from the GALEN project) and better knowledge representation for each procedure. PMID:21346941

  5. Evaluation of different counting methods for use in radiochemical purity testing procedures for 99mTc-labelled radiopharmaceuticals.

    Science.gov (United States)

    Maioli, Claudio; Bestetti, Alberto; Milani, Franco; Cornalba, Gian Paolo; Tagliabue, Luca; Di Benedetto, Domenica; Rognoni, Ilaria; Luigi Tarolo, Gian; Paroni, Rita

    2008-04-01

    The efficiency and accuracy of different methods for quality control of radiopharmaceutical preparations for diagnostic purpose were studied. The radiochemical purity of (99m)Tc Tetrafosmin, (99m)Tc Exametazime, (99m)Tc Sestamibi and (99m)Tc Oxidronate was evaluated by different thin layer chromatography systems, followed by cutting of the strips into two or three sections and by the measurement of radioactivity distribution by dose calibrator or gamma counter. In addition, to confirm the accuracy of these routine procedures, the strips were cut into a number of micro-sections (14-25) and each of them evaluated by the gamma counter. The three tested procedures gave similar results and revealed a good and comparable accuracy. The radioactivity measurement with the dose calibrator remains the most practicable because of the rapidity of execution.

  6. Use of the QR Reader to Provide Real-Time Evaluation of Residents' Skills Following Surgical Procedures.

    Science.gov (United States)

    Reynolds, Kellin; Barnhill, Danny; Sias, Jamie; Young, Amy; Polite, Florencia Greer

    2014-12-01

    A portable electronic method of providing instructional feedback and recording an evaluation of resident competency immediately following surgical procedures has not previously been documented in obstetrics and gynecology. This report presents a unique electronic format that documents resident competency and encourages verbal communication between faculty and residents immediately following operative procedures. The Microsoft Tag system and SurveyMonkey platform were linked by a 2-D QR code using Microsoft QR code generator. Each resident was given a unique code (TAG) embedded onto an ID card. An evaluation form was attached to each resident's file in SurveyMonkey. Postoperatively, supervising faculty scanned the resident's TAG with a smartphone and completed the brief evaluation using the phone's screen. The evaluation was reviewed with the resident and automatically submitted to the resident's educational file. The evaluation system was quickly accepted by residents and faculty. Of 43 residents and faculty in the study, 38 (88%) responded to a survey 8 weeks after institution of the electronic evaluation system. Thirty (79%) of the 38 indicated it was superior to the previously used handwritten format. The electronic system demonstrated improved utilization compared with paper evaluations, with a mean of 23 electronic evaluations submitted per resident during a 6-month period versus 14 paper assessments per resident during an earlier period of 6 months. This streamlined portable electronic evaluation is an effective tool for direct, formative feedback for residents, and it creates a longitudinal record of resident progress. Satisfaction with, and use of, this evaluation system was high.

  7. Evaluation of ride quality measurement procedures by subjective experiments using simulators

    Science.gov (United States)

    Klauder, L. T., Jr.; Clevenson, S. A.

    1975-01-01

    Since ride quality is, by definition, a matter of passenger response, there is need for a qualification procedure (QP) for establishing the degree to which any particular ride quality measurement procedure (RQMP) does correlate with passenger responses. Once established, such a QP will provide very useful guidance for optimal adjustment of the various parameters which any given RQMP contains. A QP is proposed based on use of a ride motion simulator and on test subject responses to recordings of actual vehicle motions. Test subject responses are used to determine simulator gain settings for the individual recordings such as to make all of the simulated rides equally uncomfortable to the test subjects. Simulator platform accelerations vs. time are recorded with each ride at its equal discomfort gain setting. The equal discomfort platform acceleration recordings are then digitzed.

  8. An Evaluation of a Proposed Revision of the ASTM D 1990 Grouping Procedure

    Science.gov (United States)

    Steve P Verrill; James W. Evans; David E. Kretschmann; Cherilyn A. Hatfield

    2013-01-01

    Lum, Taylor, and Zidek have proposed a revised procedure for wood species grouping in ASTM standard D 1990. We applaud the authors’ recognition of the importance of considering a strength distribution’s variability as well as its fifth percentile. However, we have concerns about their proposed method of incorporating this information into a standard. We detail these...

  9. Preparation and Evaluation of Dexamethasone (DEX/Growth and Differentiation Factor-5 (GDF-5 Surface-Modified Titanium Using β-Cyclodextrin-Conjugated Heparin (CD-Hep for Enhanced Osteogenic Activity In Vitro and In Vivo

    Directory of Open Access Journals (Sweden)

    Dae Hyeok Yang

    2017-08-01

    Full Text Available The most ideal implant models in the dental and orthopedic fields to minimize the failure rate of implantation involve the improvement of osseointegration with host bone. Therefore, a focus of this study is the preparation of surface-modified titanium (Ti samples of disc and screw types using dexamethasone (DEX and/or growth and differentiation factor-5 (GDF-5, as well as the evaluation of their efficacies on bone formation in vitro and in vivo. X-ray photoelectron spectroscopy (XPS, scanning electron microscopy (SEM and contact angle measurement were used to evaluate the surface chemical composition, surface morphology and wettability, respectively. The results showed that implant surfaces were successfully modified with DEX and/or GDF-5, and had rough surfaces along with hydrophilicity. DEX, GDF-5 or DEX/GDF-5 on the surface-modified samples were rapidly released within one day and released for 28 days in a sustained manner. The proliferation and bone formation of MC3T3-E1 cells cultured on pristine and surface-modified implants in vitro were examined by cell counting kit-8 (CCK-8 assay, as well as the measurements of alkaline phosphatase (ALP activity and calcium deposition, respectively. MC3T3-E1 cells cultured on DEX/GDF-5–Ti showed noticeable ALP activity and calcium deposition in vitro. Active bone formation and strong osseointegration occurred at the interface between DEX/GDF-5–Ti and host bone, as evaluated by micro computed-tomography (micro CT analysis. Surface modification using DEX/GDF-5 could be a good method for advanced implants for orthopaedic and dental applications.

  10. Artesunate enhances γδ T-cell-mediated antitumor activity through augmenting γδ T-cell function and reversing immune escape of HepG2 cells.

    Science.gov (United States)

    Qian, Peng; Zhang, Yong-Wen; Zhou, Zhong-Hai; Liu, Jun-Quan; Yue, Su-Yang; Guo, Xiang-Li; Sun, Lei-Qing; Lv, Xiao-Ting; Chen, Jian-Qun

    2018-02-06

    To explore the effect and mechanism of artesunate on γδ T cell-mediated antitumor immune responses against hepatoma carcinoma cells (HepG2) in vitro. Human γδ T cells or HepG2 were respectively treated with artesunate, subjected to co-culture as appropriate, and the following assays were subsequently conducted: CCK8 to examine cell viability; LDH release assay to detect the killing effect of γδ T cells on HepG2 cells; flow cytometry to examine the expression of perforin (PFP) and granzyme B (GraB) of γδ T cells; ELISA to evaluate the levels of TGF-β1 and IL-10 in the collected supernatant of HepG2 cells pretreated with artesunate; and Western blot analysis to examine Fas, FasL, STAT3, p-STAT3 expression of HepG2 cells induced by artesunate.  Results: The results showed that the cytotoxicity effect of γδ T cells pretreated with artesunate on HepG2 cells was augmented via elevating the expression of GraB in γδ T cells. Furthermore, treatment with artesunate reversed the inhibition of HepG2 cells on γδ T cells by reducing the secretion of TGF-β1 in HepG2 cells supernatant and enhanced the antitumor effect of γδ T cells against HepG2 cells through increasing the expression of Fas on HepG2 cells, which may be attributed to the inhibition of STAT3 signaling protein. Artesunate has several mechanisms for augmenting the antitumor immune responses mediated by γδ T cells. These results suggested artesunate may be an efficacious agent in the treatment of hepatocellular carcinoma.

  11. HEP-FCE Working Group on Libraries and Tools

    CERN Document Server

    Borgland, Anders; Kirby, Michael; Patton, Simon; Potekhin, Maxim; Viren, Brett; Yanny, Brian

    2015-01-01

    This is a report from the Libraries and Tools Working Group of the High Energy Physics Forum for Computational Excellence. It presents the vision of the working group for how the HEP software community may organize and be supported in order to more efficiently share and develop common software libraries and tools across the world's diverse set of HEP experiments. It gives prioritized recommendations for achieving this goal and provides a survey of a select number of areas in the current HEP software library and tools landscape. The survey identifies aspects which support this goal and areas with opportunities for improvements. The survey covers event processing software frameworks, software development, data management, workflow and workload management, geometry information management and conditions databases.

  12. Acetaminophen hepatotoxicity and mechanisms of its protection by N-acetylcysteine: a study of Hep3B cells.

    Science.gov (United States)

    Manov, Irena; Hirsh, Mark; Iancu, Theodore C

    2002-02-01

    Acetaminophen (AAP) hepatotoxicity, resulting in centrilobular necrosis, is frequently encountered following suicidal attempts, especially by adolescents, but also after its excessive use in infants. The subcellular and molecular sequences leading to hepatocellular cell death are not yet clear. We therefore investigated AAP hepatotoxicity by using cultured hepatoma-derived cells (Hep3B) exposed to AAP and N-acetylcysteine (NAC), used as a protective agent. Specifically, we studied the role of apoptosis and oxidative damage as putative mechanisms of AAP-associated cytotoxicity. Hep3B cells were exposed to AAP (5-25 mM) and NAC (5 mM) for different time periods. Cell viability was assessed by the Alamar Blue Reduction Test and LDH. Oxidative damage was evaluated by measuring reactive oxygen species (ROS) and glutathione. AAP-induced apoptosis was investigated by flow cytometry and transmission electron microscopy. We found that: 1. In Hep3B cells, AAP causes a time- and concentration-dependent cytotoxic effect, leading to oxidative stress, mitochondrial dysfunction, alterations of membrane permeability and apoptosis; 2. In the course of AAP cytotoxicity, the generation of ROS appears as an early event which precedes decrease of viability, LDH leakage, glutathione depletion and apoptosis; 3. NAC protects Hep3B cells from AAP-induced oxidative injury, but does not prevent apoptosis.

  13. Effects of the radiolysis products of sennoside A on HepG2 and PC-3 cell

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dong Ho; Jo, Min Ho [Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup (Korea, Republic of)

    2016-11-15

    Radiolysis of sennoside A was carried out by gamma irradiation and the anti-cancer activities of the radiolysis product were evaluated. An aqueous solution of sennoside A was exposed to 0.5-3 kGy of gamma irradiation and the radiolysis products were analyzed by HPLC. A fraction of radiolysis product (RLF) of sennoside A was isolated and the RLF was presumed as a rhein-8-β-D-glucoside. The anticancer effect of the RLF was compared with the sennoside and rhein using a in vitro assay system of human prostate cancer cells (PC-3) and human hepatoma HepG2 cells. The cell viability of PC-3 and HepG2 cell was significantly decreased to 12.4±1.2% and 32.4±2.1%, respectively, by the treatment of 0.6 μM of RLF. The sennoside A (range from 0 to 25 μM) had no cytotoxic effect on PC-3 and HepG2 cells, while the rhein had the effect on HepG2 cells with a LD{sub 50} at 80 μM.

  14. Evaluation of an interlaced triple procedure: penetrating keratoplasty, extracapsular cataract extraction, and nonopen-sky intraocular lens implantation.

    Science.gov (United States)

    Yang, Shuo; Wang, Bin; Zhang, Yangyang; Zhai, Hualei; Wang, Junyi; Wang, Shuang; Xie, Lixin

    2017-09-01

    To evaluate an interlaced triple procedure that involved penetrating keratoplasty (PKP), extracapsular cataract extraction (ECCE) using diathermy capsulotomy, and nonopen-sky intraocular lens (IOL) implantation.This retrospective study involved data from 34 patients who were diagnosed with severe corneal opacities and cataracts. These patients were divided into an interlaced procedure group (21 patients) and a traditional procedure group (13 patients). In the interlaced group, the method of continuous curvilinear capsulorhexis (CCC) was completed via diathermy capsulotomy. The donor corneal button was sutured at 8 positions (at equal intervals) using 10-0 nylon sutures, and the IOL was inserted into the capsular bag using a closed anterior chamber approach at the 10:30 to 12 o'clock positions between the sutures. In the traditional group, CCC was completed using side-port capsular forceps, and the IOL was implanted using an open anterior chamber approach.In the interlaced group, the CCC, open-sky, and total operation times were significantly shorter than in the traditional group (P < .05). Neither the best-corrected visual acuity (BCVA) nor corneal endothelial cell density was significantly different between the groups at 1 and 6 months after the operation.This interlaced triple procedure for the treatment of corneal diseases with cataracts appears to be feasible and practical.

  15. Evaluating the Strange Situation Procedure (SSP to assess the bond between dogs and humans.

    Directory of Open Access Journals (Sweden)

    Therese Rehn

    Full Text Available The Strange Situation Procedure (SSP is increasingly being used to study attachment between dogs and humans. It has been developed from the Ainsworth Strange Situation Procedure, which is used extensively to investigate attachment between children and their parents. In this experiment, 12 female beagle dogs were tested in two treatments to identify possible order effects in the test, a potential weakness in the SSP. In one treatment (FS, dogs participated together with a 'familiar person' and a 'stranger'. In a control treatment (SS, the same dogs participated together with two unfamiliar people, 'stranger A' and 'stranger B'. Comparisons were made between episodes within as well as between treatments. As predicted in FS, dogs explored more in the presence of the familiar person than the stranger. Importantly, they also explored more in the presence of stranger A (who appeared in the same order as the familiar person and followed the same procedure than stranger B in SS. Furthermore, comparisons between treatments, where a familiar person was present in FS and stranger A was present in SS, showed no differences in exploration. In combination, these results indicate that the effect of a familiar person on dogs' exploratory behaviour, a key feature when assessing secure attachment styles, could not be tested reliably due to the order in which the familiar person and the stranger appear. It is proposed that in the future only counterbalanced versions of the SSP are used. Alternatively, since dogs reliably initiated more contact with the familiar person compared to the strangers, it is suggested that future studies on attachment in dogs towards humans should focus either on the behaviour of the dog in those episodes of the SSP when the person returns, or on reunion behaviour in other studies, specially designed to address dog-human interactions at this time.

  16. Evaluating the Strange Situation Procedure (SSP) to Assess the Bond between Dogs and Humans

    Science.gov (United States)

    Rehn, Therese; McGowan, Ragen T. S.; Keeling, Linda J.

    2013-01-01

    The Strange Situation Procedure (SSP) is increasingly being used to study attachment between dogs and humans. It has been developed from the Ainsworth Strange Situation Procedure, which is used extensively to investigate attachment between children and their parents. In this experiment, 12 female beagle dogs were tested in two treatments to identify possible order effects in the test, a potential weakness in the SSP. In one treatment (FS), dogs participated together with a ‘familiar person’ and a ‘stranger’. In a control treatment (SS), the same dogs participated together with two unfamiliar people, ‘stranger A’ and ‘stranger B’. Comparisons were made between episodes within as well as between treatments. As predicted in FS, dogs explored more in the presence of the familiar person than the stranger. Importantly, they also explored more in the presence of stranger A (who appeared in the same order as the familiar person and followed the same procedure) than stranger B in SS. Furthermore, comparisons between treatments, where a familiar person was present in FS and stranger A was present in SS, showed no differences in exploration. In combination, these results indicate that the effect of a familiar person on dogs' exploratory behaviour, a key feature when assessing secure attachment styles, could not be tested reliably due to the order in which the familiar person and the stranger appear. It is proposed that in the future only counterbalanced versions of the SSP are used. Alternatively, since dogs reliably initiated more contact with the familiar person compared to the strangers, it is suggested that future studies on attachment in dogs towards humans should focus either on the behaviour of the dog in those episodes of the SSP when the person returns, or on reunion behaviour in other studies, specially designed to address dog-human interactions at this time. PMID:23437277

  17. Design and evaluation of computerized operating procedures in nuclear power plants.

    Science.gov (United States)

    Hwang, Fei-Hui; Hwang, Sheue-Ling

    2003-01-15

    A small-scale virtual system has been developed in this study to enhance operators' understanding and operating performance. For this, a computerized graphical interface based on Dynamic Work Causality Equation (DWCE) has been designed to transform the operating procedure into a flowchart. Furthermore, the Programmable Logic Controller (PLC) was installed to connect the signboard (proposed system) with the computerized graphical interface. An experiment was conducted to verify the effect of computerized graphic interface, indicating that the computerized system significantly decreases learning time and improves operational performance.

  18. Java Analysis Studio and the hep.lcd class library

    Energy Technology Data Exchange (ETDEWEB)

    Ronan, M.T.

    2000-02-14

    The Java Analysis Studio and the hep.lcd class library provide a general framework for performing Java-based Linear Collider Detector (LCD) studies. The package is being developed to fully reconstruct 500 GeV to 1.5 TeV e{sup +}e{sup {minus}} annihilation events for analyzing detector options and performance. The current North American LCD reconstruction effort is aimed at comparing different detailed detector models by performing full detector simulation and reconstruction. This paper describes the JAS/hep.lcd distributed analysis framework and some aspects of the reconstruction and analysis object modeling.

  19. Encefalopatia hepática: estudo de 50 casos

    Directory of Open Access Journals (Sweden)

    Luiz Antonio de Lima Resende

    1984-12-01

    Full Text Available Os autores apresentam estudo retrospectivo de 50 pacientes com encefalopatia hepática atendidos no Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto a partir de 1959 e discutem os aspectos clínicos, laboratoriais e anátomo-patológicos. Expõem os diagnósticos das hepatopatias, os fatores precipitantes dos episódios de coma e pré-coma hepático e enfatizam o papel da gasometria arterial no estabelecimento do diagnóstico.

  20. Esporotricose experimental sistêmica em ratos Wistar: avaliação hematológica e perfil hepático Systemic experimental sporotrichosis in Wistar rats: hematological evaluation and hepatic profile

    Directory of Open Access Journals (Sweden)

    A.R.M. Meinerz

    2008-08-01

    Full Text Available Alanine aminotransferase (ALT and alkaline phosphatase (AP hepatic enzymes, hematological values, and total plasmatic proteins (TPP of 12 Wistar rats with systemic experimental sporotrichosis were evaluated. Inoculation was performed at 2x10³ cells of S. schenckii/ml. The values ranged from 35 to 48UI/l for ALT and 67 to 251.3UI/l for AP. Fifty percent of the AP values were above the physiological limits. TPP was elevated in 66.7% (8/12 of the rats with values varying from 7.9 to 8.5g%. In 75% (9/12 of the animals, it was observed that hemoglobin and red blood cells count were below the physiologic limits, and 8.3% (1/12 of the animals showed anemia masked by dehydration. At the necropsy, it was observed local and/or disseminated nodular lesions. At the histological analysis, cells compatible to S. schenckii were found. The observed alterations are aggravating factors for the recurrence and treatment of the mycosis.

  1. Beyond Satisfaction: Toward an Outcomes-Based, Procedural Model of Faculty Development Program Evaluation

    Science.gov (United States)

    Brooks, D. Christopher; Marsh, Lauren; Wilcox, Kimerly; Cohen, Brad

    2011-01-01

    In response to the well-documented need for rigorous evaluations of faculty development programs and increasing demands for institutional accountability, University of Minnesota's Office of Information Technology (OIT) researchers have developed an approach to program evaluation that assesses individual level changes to participants' attitudes,…

  2. Comparative ultrasound evaluation of human trabecular bone graft properties after treatment with different sterilization procedures.

    Science.gov (United States)

    Vastel, L; Masse, C; Mesnil, P; Crozier, E; Padilla, F; Laugier, P; Mitton, D; Courpied, J P

    2009-07-01

    New sterilization methods for human bone are likely to affect the mechanical properties of human cancellous grafts. These mechanical properties dictate the short- and mid-term results of the orthopedic procedure. The aim of this study was to compare the effects on bone mechanical properties, as assessed by ultrasound velocity, of different sterilization methods used under similar conditions: bleach and sublimation, humid heat, successive baths of physiological saline with osmotic detersion, and CO(2) in the supercritical phase. Alterations in mechanical properties were small with CO(2) (velocity change: -2%) and humid heat (-2.5%). Osmotic detersion had a significant but moderate effect (-4.7%). The -9% change with the protocol involving bleach suggested a greater than 30% decrease in load to failure, based on earlier studies. Gamma irradiation of defatted trabecular allografts, in a dose of 10 or 25 KGy, produced no significant changes in ultrasound velocity. Powerful protein denaturants used in sterilization protocols substantially alter the mechanical resistance of the grafts, which may jeopardize the orthopedic procedure. (c) 2009 Wiley Periodicals, Inc.

  3. Effects of PARP-1 inhibitors AG-014699 and AZD2281 on proliferation and apoptosis of human hepatoma cell line HepG2

    Directory of Open Access Journals (Sweden)

    DU Senrong

    2015-06-01

    Full Text Available ObjectiveTo observe the inhibitory and pro-apoptotic effects of two poly(ADP-ribose polymerase (PARP-1 inhibitors, AG-014699 and AZD2281, on human hepatoma HepG2 cells and preliminarily explore the mechanism by which AG-014699 induces HepG2 cell apoptosis, and to provide a new therapeutic target for hepatoma. MethodsThe effects of different concentrations of AG-014699 and AZD2281 on HepG2 cell proliferation were determined by MTT assay. The cell apoptosis rate was measured by flow cytometry. The expression levels of caspase-3 and caspase-8 were measured by Western Blot. Inter-group comparison was made by t test. ResultsBoth AG-014699 and AZD2281 suppressed HepG2 cell proliferation in a time- and dose-dependent manner. However, the sensitivity of HepG2 cells to the two PARP-1 inhibitors was different. The half-maximal inhibitory concentrations of AG-014699 and AZD2281 at 48 h determined by MTT assay were about 20 μmol/L and 400 μmol/L, respectively. Flow cytometry and Western blot were not used to evaluate the apoptosis of HepG2 cells exposed to AZD2281 to which these cells were not sensitive. HepG2 cell apoptosis could be induced by 10, 30, and 50 μmol/L AG-014699, and the highest apoptosis rate at 48 h was significantly higher than that of the control group (3100%±2.13% vs 09%±0013%, P<0.01. Compared with those in the control group, the protein levels of caspase-3 and caspase-8 in HepG2 cells after 48-h exposure to 30, and 50 μmol/L AG-014699 increased. ConclusionThe two PARP-1 inhibitors AG-014699 and AZD2281 can inhibit the proliferation of HepG2 cells, which showed different sensitivities to the two inhibitors. AG-014699 can induce HepG2 cell apoptosis by up-regulating the protein expression of caspase-3 and caspase-8.

  4. Alteraciones hepáticas inducidas por la nutrición parenteral

    OpenAIRE

    J Salas Salvado; A Recaséns Garica

    1993-01-01

    Liver disorders induced by parenteral nutrition Alteraciones hepáticas inducidas por la nutrición parenteral Liver disorders induced by parenteral nutrition Alteraciones hepáticas inducidas por la nutrición parenteral

  5. Emergency evacuation/transportation plan update: Traffic model development and evaluation of early closure procedures. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1993-10-28

    Prolonged delays in traffic experienced by Laboratory personnel during a recent early dismissal in inclement weather, coupled with reconstruction efforts along NM 502 east of the White Rock Wye for the next 1 to 2 years, has prompted Los Alamos National Laboratory (LANL) to re-evaluate and improve the present transportation plan and its integration with contingency plans maintained in other organizations. Facilities planners and emergency operations staff need to evaluate the transportation system`s capability to inefficiently and safely evacuate LANL under different low-level emergency conditions. A variety of potential procedures governing the release of employees from the different technical areas (TAs) requires evaluation, perhaps with regard to multiple emergency-condition scenarios, with one or more optimal procedures ultimately presented for adoption by Lab Management. The work undertaken in this project will hopefully lay a foundation for an on-going, progressive transportation system analysis capability. It utilizes microscale simulation techniques to affirm, reassess and validate the Laboratory`s Early Dismissal/Closure/Delayed Opening Plan. The Laboratory is required by Federal guidelines, and compelled by prudent practice and conscientious regard for the welfare of employees and nearby residents, to maintain plans and operating procedures for evacuation if the need arises. The tools developed during this process can be used outside of contingency planning. It is anticipated that the traffic models developed will allow site planners to evaluate changes to the traffic network which could better serve the normal traffic levels. Changes in roadway configuration, control strategies (signalization and signing), response strategies to traffic accidents, and patterns of demand can be modelled using the analysis tools developed during this project. Such scenarios typically are important considerations in master planning and facilities programming.

  6. Evaluation of a robotic arm for echocardiography to X-ray image registration during cardiac catheterization procedures.

    Science.gov (United States)

    Ma, Yingliang; Penney, Graeme P; Bos, Dennis; Frissen, Peter; de Fockert, George; King, Andy; Gao, Gang; Yao, Cheng; Totman, John; Ginks, Matthew; Rinaldi, C; Razavi, Reza; Rhode, Kawal S

    2009-01-01

    We present an initial evaluation of a robotic arm for positioning a 3D echo probe during cardiac catheterization procedures. By tracking the robotic arm, X-ray table and X-ray C-arm, we are able to register the 3D echo images with live 2D X-ray images. In addition, we can also use tracking data from the robotic arm combined with system calibrations to create extended field of view 3D echo images. Both these features can be used for roadmapping to guide cardiac catheterization procedures. We have carried out a validation experiment of our registration method using a cross-wire phantom. Results show our method to be accurate to 3.5 mm. We have successfully demonstrated the creation of the extended field of view data on 2 healthy volunteers and the registration of echo and X-ray data on 1 patient undergoing a pacing study.

  7. Evaluation of the behavioral characteristics of the mdx mouse model of duchenne muscular dystrophy through operant conditioning procedures.

    Science.gov (United States)

    Lewon, Matthew; Peters, Christina M; Van Ry, Pam M; Burkin, Dean J; Hunter, Kenneth W; Hayes, Linda J

    2017-09-01

    The mdx mouse is an important nonhuman model for Duchenne muscular dystrophy (DMD) research. Characterizing the behavioral traits of the strain relative to congenic wild-type (WT) mice may enhance our understanding of the cognitive deficits observed in some humans with DMD and contribute to treatment development and evaluation. In this paper we report the results of a number of experiments comparing the behavior of mdx to WT mice in operant conditioning procedures designed to assess learning and memory. We found that mdx outperformed WT in all learning and memory tasks involving food reinforcement, and this appeared to be related to the differential effects of the food deprivation motivating operation on mdx mice. Conversely, WT outperformed mdx in an escape/avoidance learning task. These results suggest motivational differences between the strains and demonstrate the potential utility of operant conditioning procedures in the assessment of the behavioral characteristics of the mdx mouse. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Some fundamental aspects of fault-tree and digraph-matrix relationships for a systems-interaction evaluation procedure

    Energy Technology Data Exchange (ETDEWEB)

    Alesso, H.P.

    1982-02-28

    Recent events, such as Three Mile Island-2, Brown's Ferry-3, and Crystal River-3, have demonstrated that complex accidents can occur as a result of dependent (common-cause/mode) failures. These events are now being called Systems Interactions. A procedure for the identification and evaluation of Systems Interactions is being developed by the NRC. Several national laboratories and utilities have contributed preliminary procedures. As a result, there are several important views of the Systems Interaction problem. This report reviews some fundamental mathematical background of both fault-oriented and success-oriented risk analyses in order to bring out the advantages and disadvantages of each. In addition, it outlines several fault-oriented/dependency analysis approaches and several success-oriented/digraph-matrix approaches. The objective is to obtain a broad perspective of present options for solving the Systems Interaction problem.

  9. Pragmatic evaluation of the Toyota Production System (TPS analysis procedure for problem solving with entry-level nurses

    Directory of Open Access Journals (Sweden)

    Lukasz Maciej Mazur

    2008-12-01

    Full Text Available Medication errors occurring in hospitals are a growing national concern. These medication errors and their related costs (or wastes are seen as major factors leading to increased patient safety risks and increased waste in the hospital setting.  This article presents a study in which sixteen entry-level nurses utilized a Toyota Production System (TPS analysis procedure to solve medication delivery problems at one community hospital. The objective of this research was to study and evaluate the TPS analysis procedure for problem solving with entry-level nurses. Personal journals, focus group discussions, and a survey study were used to collect data about entry-level nurses’ perceptions of using the TPS problem solving approach to study medication delivery. A regression analysis was used to identify characteristics that enhance problem solving efforts. In addition, propositions for effective problem solving by entry-level nurses to aid in the reduction of medication errors in healthcare delivery settings are offered.

  10. Evaluating newly acquired authority of nurse practitioners and physician assistants for reserved medical procedures in the Netherlands: a study protocol.

    Science.gov (United States)

    De Bruijn-Geraets, Daisy P; Van Eijk-Hustings, Yvonne J L; Vrijhoef, Hubertus J M

    2014-11-01

    The study protocol is designed to evaluate the effects of granting independent authorization for medical procedures to nurse practitioners and physician assistants on processes and outcomes of health care. Recent (temporarily) enacted legislation in Dutch health care authorizes nurse practitioners and physician assistants to indicate and perform specified medical procedures, i.e. catheterization, cardioversion, defibrillation, endoscopy, injection, puncture, prescribing and simple surgical procedures, independently. Formerly, these procedures were exclusively reserved to physicians, dentists and midwives. A triangulation mixed method design is used to collect quantitative (surveys) and qualitative (interviews) data. Outcomes are selected from evidence-based frameworks and models for assessing the impact of advanced nursing on quality of health care. Data are collected in various manners. Surveys are structured around the domains: (i) quality of care; (ii) costs; (iii) healthcare resource use; and (iv) patient centredness. Focus group and expert interviews aim to ascertain facilitators and barriers to the implementation process. Data are collected before the amendment of the law, 1 and 2·5 years thereafter. Groups of patients, nurse practitioners, physician assistants, supervising physicians and policy makers all participate in this national study. The study is supported by a grant from the Dutch Ministry of Health, Welfare and Sport in March 2011. Research Ethics Committee approval was obtained in July 2011. This study will provide information about the effects of granting independent authorization for medical procedures to nurse practitioners and physician assistants on processes and outcomes of health care. Study findings aim to support policy makers and other stakeholders in making related decisions. The study design enables a cross-national comparative analysis. © 2014 The Authors. Journal of Advanced Nursing Published by John Wiley & Sons Ltd.

  11. Student Evaluation of Friedman's Immediate Feedback, No Return Test Procedure for Introductory Psychology.

    Science.gov (United States)

    Smith, Randolph A.; Wight, Randall

    1988-01-01

    Friedman's (1987) immediate feedback, no return test technique was evaluated via student ratings. Students were enthusiastic in their assessment of the technique and believed that it facilitated their learning. (Author)

  12. Clinical evaluation of a dermic allograft in procedures to increase attached gingiva width

    OpenAIRE

    Vieira,Ésio de Oliveira; Fidel Junior,Rivail Antonio Sergio; Figueredo,Carlos Marcelo da Silva; Fischer, Ricardo Guimarães

    2009-01-01

    The aim of this study was to clinically evaluate the use of alloderm to increase the width of attached gingiva. Nine patients were selected. The inclusion criteria were: attached gingiva smaller than 1 mm; Miller's class I and II gingival recession; patients able to attend control dental appointments; absence of periodontal pocket and endodontic treatment in the neighboring area where the acellular dermal graft would be placed. The clinical evaluation included: allograft shrinkage 7, 14, 21, ...

  13. Status of JENDL High Energy File. Evaluation method, tools, specification, release procedure, etc

    Energy Technology Data Exchange (ETDEWEB)

    Fukahori, Tokio [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1998-11-01

    The ENDF-6 format file should be kept as a standard distribution file and it is not difficult to convert into some other form for code`s libraries. From this point of view, status of JENDL High Energy File is introduced in this report as well as evaluation strategy, recommended specification, stored nuclides and quantities, a format structure, evaluation methods and tools, and release plan. (author)

  14. Phase IV, Open-Label, Safety Study Evaluating the Use of Dexmedetomidine in Pediatric Patients Undergoing Procedure-Type Sedation

    Directory of Open Access Journals (Sweden)

    Edmund H. Jooste

    2017-08-01

    Full Text Available Dexmedetomidine (Precedex™ may be used as an alternative sedative in children, maintaining spontaneous breathing, and avoiding tracheal intubation in a non-intubated moderate or deep sedation (NI-MDS approach. This open-label, single-arm, multicenter study evaluated the safety of dexmedetomidine in a pediatric population receiving NI-MDS in an operating room or a procedure room, with an intensivist or anesthesiologist in attendance, for elective diagnostic or therapeutic procedures expected to take at least 30 min. The primary endpoint was incidence of treatment-emergent adverse events (TEAEs. Patients received one of two doses dependent on age: patients aged ≥28 weeks' gestational age to <1 month postnatal received dose level 1 (0.1 μg/kg load; 0.05–0.2 μg/kg/h infusion; those aged 1 month to <17 years received dose level 2 (1 μg/kg load; 0.2–2.0 μg/kg/h infusion. Sedation efficacy was assessed and defined as adequate sedation for at least 80% of the time and successful completion of the procedure without the need for rescue medication. In all, 91 patients were enrolled (dose level 1, n = 1; dose level 2, n = 90; of these, 90 received treatment and 82 completed the study. Eight patients in dose level 2 discontinued treatment for the following reasons: early completion of diagnostic or therapeutic procedure (n = 3; change in medical condition (need for intubation requiring deeper level of sedation (n = 2; adverse event (AE; hives and emesis, lack of efficacy, and physician decision (patient not sedated enough to complete procedure; n = 1 each. Sixty-seven patients experienced 147 TEAEs. The two most commonly reported AEs were respiratory depression (bradypnea; reported per protocol-defined criteria, based on absolute respiratory rate values for age or relative decrease of 30% from baseline and hypotension. Four patients received glycopyrrolate for bradycardia and seven patients received intravenous fluids for hypotension. SpO2

  15. Development of a procedure for evaluating the economics of process options in commercial HTGR fuel cycle

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, W.E.

    1976-05-01

    The report presented describes the status of and calculational procedures used in a machine code being developed for calculating costs of fresh fuel fabrication, chemical reprocessing, and refabrication of HTGR fuel. In its present state of development, the capability is confined principally to calculating annual mainstream and scrap recovery process throughputs in fresh fuel fabrication, chemical reprocessing, and refabrication. The code works from user-supplied reactor build schedules and fuel specifications to approximate changing fuel fabrication requirements over a 20-year (maximum) projection. The availability of a reprocessing facility may be delayed to some specified date beyond the first year of a projection. Some of the cost routines have also been programmed and are described.

  16. Semiotics and semiology of Nursing: evaluation of undergraduate students' knowledge on procedures.

    Science.gov (United States)

    Melo, Gabriela de Sousa Martins; Tibúrcio, Manuela Pinto; Freitas, Camylla Cavalcante Soares de; Vasconcelos, Quinídia Lúcia Duarte de Almeida Quithé de; Costa, Isabel Karolyne Fernandes; Torres, Gilson de Vasconcelos

    2017-04-01

    to assess the knowledge of scholars on Nursing regarding simple hands hygiene (SHH), blood pressure measurement (BP), peripheral venipuncture (PV) with venous catheter and male urethral catheterization delay (UCD) procedures. quantitative study carried out between February and May 2014, with 186 undergraduate Nursing students from 5th to 9th period of a public university of Rio Grande do Norte, with application of four questionnaires. One carried out descriptive and analytic analysis. the students presented low average percentage of right answers, especially in blood pressure measurement (55.5%); SHH's average was higher than 70%. The average of correct answers was the highest in SHH (8.6), followed by UCD (7.8), PV (7.4) and BP (6.7). The questions regarding the topic "concepts" showed less correct answers when comparing it to the topic "technique steps". it is necessary to establish knowledge monitoring strategies, in order to stimulate the constant improvement.

  17. Battery Separator Characterization and Evaluation Procedures for NASA's Advanced Lithium-Ion Batteries

    Science.gov (United States)

    Baldwin, Richard S.; Bennet, William R.; Wong, Eunice K.; Lewton, MaryBeth R.; Harris, Megan K.

    2010-01-01

    To address the future performance and safety requirements for the electrical energy storage technologies that will enhance and enable future NASA manned aerospace missions, advanced rechargeable, lithium-ion battery technology development is being pursued within the scope of the NASA Exploration Technology Development Program s (ETDP's) Energy Storage Project. A critical cell-level component of a lithium-ion battery which significantly impacts both overall electrochemical performance and safety is the porous separator that is sandwiched between the two active cell electrodes. To support the selection of the optimal cell separator material(s) for the advanced battery technology and chemistries under development, laboratory characterization and screening procedures were established to assess and compare separator material-level attributes and associated separator performance characteristics.

  18. Selenium speciation in phosphate mine soils and evaluation of a sequential extraction procedure using XAFS

    Energy Technology Data Exchange (ETDEWEB)

    Favorito, Jessica E.; Luxton, Todd P.; Eick, Matthew J.; Grossl, Paul R. (VP); (Utah SU); (EPA)

    2017-10-01

    Selenium is a trace element found in western US soils, where ingestion of Se-accumulating plants has resulted in livestock fatalities. Therefore, a reliable understanding of Se speciation and bioavailability is critical for effective mitigation. Sequential extraction procedures (SEP) are often employed to examine Se phases and speciation in contaminated soils but may be limited by experimental conditions. We examined the validity of a SEP using X-ray absorption spectroscopy (XAS) for both whole and a sequence of extracted soils. The sequence included removal of soluble, PO4-extractable, carbonate, amorphous Fe-oxide, crystalline Fe-oxide, organic, and residual Se forms. For whole soils, XANES analyses indicated Se(0) and Se(-II) predominated, with lower amounts of Se(IV) present, related to carbonates and Fe-oxides. Oxidized Se species were more elevated and residual/elemental Se was lower than previous SEP results from ICP-AES suggested. For soils from the SEP sequence, XANES results indicated only partial recovery of carbonate, Fe-oxide and organic Se. This suggests Se was incompletely removed during designated extractions, possibly due to lack of mineral solubilization or reagent specificity. Selenium fractions associated with Fe-oxides were reduced in amount or removed after using hydroxylamine HCl for most soils examined. XANES results indicate partial dissolution of solid-phases may occur during extraction processes. This study demonstrates why precautions should be taken to improve the validity of SEPs. Mineralogical and chemical characterizations should be completed prior to SEP implementation to identify extractable phases or mineral components that may influence extraction effectiveness. Sequential extraction procedures can be appropriately tailored for reliable quantification of speciation in contaminated soils.

  19. Optical guidance for stereotactic brain tumor biopsy procedures: preliminary clinical evaluation (Conference Presentation)

    Science.gov (United States)

    Haj-Hosseini, Neda; Richter, Johan; Milos, Peter; Hallbeck, Martin; Wârdell, Karin

    2017-02-01

    In the routine of stereotactic biopsy on suspected tumors located deep in the brain or patients with multiple lesions, tissue samples are harvested to determine the type of malignancy. Biopsies are taken from pre-calculated positions based on the preoperative radiologic images susceptible to brain shift. In such cases the biopsy procedure may need to be repeated leading to a longer operation time. To provide guidance for targeting diagnostic tumor tissue and to avoid vessel rupture on the insertion path of the tumor, an application specific fiber optic probe was developed. The setup incorporated spectroscopy for 5-aminolevulinic acid induced protopophyrin IX (PpIX) fluorescence in the tumor and laser Doppler for measuring microvascular blood flow which recorded backscattered light (TLI) at 780 nm and blood perfusion. The recorded signals were compared to the histopathologic diagnosis of the tissue samples (n=16) and to the preoperative radiologic images. All together 146 fluorescence and 276 laser Doppler signals were recorded along 5 trajectories in 4 patients. On all occasions strong PpIX fluorescence peaks were visible during real-time guidance. Comparing the gliotic tumor marginal zone with the tumor, the PpIX (51 vs. 528 a.u., [0-1790], p 0.05) and blood perfusion (8.3 vs. 17 a.u., [0-254], p > 0.05) were not significantly different. In conclusion, the optical guidance probe made real-time tumor detection and vessel tracking possible during the stereotactic biopsy procedures. Moreover, the fluorescence and blood perfusion in the tumor could be studied at controlled positions in the brain and the tumor.

  20. The protective effects of carvacrol and thymol against paracetamol-induced toxicity on human hepatocellular carcinoma cell lines (HepG2).

    Science.gov (United States)

    Palabiyik, S S; Karakus, E; Halici, Z; Cadirci, E; Bayir, Y; Ayaz, G; Cinar, I

    2016-12-01

    Acetaminophen (APAP) overdose could induce liver damage and lead to acute liver failure. The treatment of APAP overdoses could be improved by new therapeutic strategies. Thymus spp., which has many beneficial effects and has been used in folk medicine, is one such potential strategy. In the present study, the hepatoprotective activity of the main constituents of Thymus spp., carvacrol and thymol, were evaluated in light of APAP-induced hepatotoxicity. We hoped to understand the hepatoprotective mechanism of these agents on the antioxidant system and pro-inflammatory cytokines in vitro. Dose-dependent effects of thymol and carvacrol (25, 50, and 100 µM) were tested on cultured HepG2 cells. N-Acetylcysteine (NAC) was tested as positive control. We showed that APAP inhibited HepG2 cell growth by inducing inflammation and oxidative stress. Incubating APAP-exposed HepG2 cells with carvacrol and thymol for 24 h ameliorated this inflammation and oxidative stress. We also evaluated alanine transaminase and lactate dehydrogenase levels of HepG2 cells. We found that thymol and carvacrol protected against APAP-induced toxicity in HepG2 cells by increasing antioxidant activity and reducing pro-inflammatory cytokines, such as tumor necrosis factor α and interleukin 1β. Taking together high-dose thymol and carvacrol treatment has an effect close to NAC treatment in APAP toxicity, but thymol has better treatment effect than carvacrol. © The Author(s) 2016.

  1. Osthole induces G2/M cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.

    Science.gov (United States)

    Chao, Xu; Zhou, Xiaojun; Zheng, Gang; Dong, Changhu; Zhang, Wei; Song, Xiaomei; Jin, Tianbo

    2014-05-01

    Osthole [7-methoxy-8-(3-methyl-2-butenyl) coumarin] isolated from the fruit of Cnidium monnieri (L.) Cuss, one of the commonly used Chinese medicines listed in the Shennong's Classic of Materia Medica in the Han Dynasty, had remarkable antiproliferative activity against human hepatocellular carcinoma HepG2 cells in culture. This study evaluated the effects of osthole on cell growth, nuclear morphology, cell cycle distribution, and expression of apoptosis-related proteins in HepG2 cells. Cytotoxic activity of osthole was determined by the MTT assay at various concentrations ranging from 0.004 to 1.0 µmol/ml in HepG2 cells. Cell morphology was assessed by Hoechst staining and fluorescence microscopy. Apoptosis and cell-cycle distribution was determined by annexin V staining and flow cytometry. Apoptotic protein levels were assessed by Western blot. Osthole exhibited significant inhibition of the survival of HepG2 cells and the half inhibitory concentration (IC₅₀) values were 0.186, 0.158 and 0.123 µmol/ml at 24, 48 and 72 h, respectively. Cells treated with osthole at concentrations of 0, 0.004, 0.02, 0.1 and 0.5 μmol/ml showed a statistically significant increase in the G2/M fraction accompanied by a decrease in the G0/G1 fraction. The increase of apoptosis induced by osthole was correlated with down-regulation expression of anti-apoptotic Bcl-2 protein and up-regulation expression of pro-apoptotic Bax and p53 proteins. Osthole had significant growth inhibitory activity and the pro-apoptotic effect of osthole is mediated through the activation of caspases and mitochondria in HepG2 cells. Results suggest that osthole has promising therapeutic potential against hepatocellular carcinoma.

  2. Galactosylated poly(ε-caprolactone) membrane promoted liver-specific functions of HepG2 cells in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yan, E-mail: zhang_yan@ecust.edu.cn [The Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237 (China); Zhang, Yi [The Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237 (China); Chen, Min; Zhou, Yan [The State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, Shanghai, 200237 (China); Lang, Meidong, E-mail: mdlang@ecust.edu.cn [The Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237 (China)

    2014-08-01

    The lack of pendant functional groups on the PCL backbone has been a great challenge for surface bioactivation of poly(ε-caprolactone) (PCL). In the present study, covalently galactosylated PCL (GPCL) was developed through coupling between the amino-functionalized PCL (NPCL) and the lactobionic acid (LA) and its potential application in maintenance of physiological functions of HepG2 cells was further evaluated. The structure and properties of GPCL were explored by {sup 1}H NMR, FT-IR, GPC and DSC. Moreover, the incorporation of galactose ligands onto GPCL membranes not only promoted higher wettability, but also radically changed surface morphology in comparison with PCL and NPCL according to the contact angle measurement and atomic force microscopy. When HepG2 cells were seeded onto these membranes, the cells on GPCL membranes showed more pronounced cell adhesion and tended to form aggregates during the initial adhesion stage and then progressively grew into multi-layer structures compared to those without galactose ligands by the observation with fluorescence microscope and scanning electron microscopy. Furthermore, live–dead assay and functional tests demonstrated that HepG2 cells on GPCL membranes had superior viability and maintained better liver-specific functions. Collectively, GPCL has great potential for hepatic tissue engineering scaffolds. - Graphical abstract: The specific recognition between the galactose ligands on the galactosylated poly(ε-caprolactone) membrane and the ASGPR on the HepG2 cell surface. The galactosylated poly(ε-caprolactone) membranes improved the cell-matrix interaction. The galactosylated functionalized PCL scaffold is a potential candidate for liver tissue engineering. - Highlights: • The specific recognition between the galactose ligands on the galactosylated poly(ε-caprolactone) membrane and the ASGPR on the HepG2 cell surface. • The galactosylated poly(ε-caprolactone) membranes improved the cell-matrix interaction.

  3. Evaluation of bias-variance trade-off for commonly used post-summarizing normalization procedures in large-scale gene expression studies.

    Science.gov (United States)

    Qiu, Xing; Hu, Rui; Wu, Zhixin

    2014-01-01

    Normalization procedures are widely used in high-throughput genomic data analyses to remove various technological noise and variations. They are known to have profound impact to the subsequent gene differential expression analysis. Although there has been some research in evaluating different normalization procedures, few attempts have been made to systematically evaluate the gene detection performances of normalization procedures from the bias-variance trade-off point of view, especially with strong gene differentiation effects and large sample size. In this paper, we conduct a thorough study to evaluate the effects of normalization procedures combined with several commonly used statistical tests and MTPs under different configurations of effect size and sample size. We conduct theoretical evaluation based on a random effect model, as well as simulation and biological data analyses to verify the results. Based on our findings, we provide some practical guidance for selecting a suitable normalization procedure under different scenarios.

  4. Evaluation of bias-variance trade-off for commonly used post-summarizing normalization procedures in large-scale gene expression studies.

    Directory of Open Access Journals (Sweden)

    Xing Qiu

    Full Text Available Normalization procedures are widely used in high-throughput genomic data analyses to remove various technological noise and variations. They are known to have profound impact to the subsequent gene differential expression analysis. Although there has been some research in evaluating different normalization procedures, few attempts have been made to systematically evaluate the gene detection performances of normalization procedures from the bias-variance trade-off point of view, especially with strong gene differentiation effects and large sample size. In this paper, we conduct a thorough study to evaluate the effects of normalization procedures combined with several commonly used statistical tests and MTPs under different configurations of effect size and sample size. We conduct theoretical evaluation based on a random effect model, as well as simulation and biological data analyses to verify the results. Based on our findings, we provide some practical guidance for selecting a suitable normalization procedure under different scenarios.

  5. Korean Byungkyul - Citrus platymamma Hort.et Tanaka flavonoids induces cell cycle arrest and apoptosis, regulating MMP protein expression in Hep3B hepatocellular carcinoma cells.

    Science.gov (United States)

    Hong, Gyeong Eun; Lee, Ho Jeong; Kim, Jin A; Yumnam, Silvia; Raha, Suchismita; Venkatarame Gowda Saralamma, Venu; Heo, Jeong Doo; Lee, Sang Joon; Kim, Eun Hee; Won, Chun Kil; Kim, Gon Sup

    2017-02-01

    Citrus platymamma Hort.et Tanaka is an indigenous fruit of Jeju island in Korea. In this study the bioactivity of C. platymamma flavonoids were evaluated on human hepatoma Hep3B cell lines. Eleven flavonoids were identified from the peels of C. platymamma Hort.et Tanaka through high-performance liquid chromatography-Tandem mass spectrometry and the anticancer effect of these C. platymamma flavonoids on human hepatoma Hep3B were studied. Chromatin condensation was observed in Hep3B cells treated with C. platymamma flavonoids. DNA fragmentation was confirmed through agarose gel electrophoresis and TUNEL assay. An increase in the total apoptotic cells and G2/M cell cycle arrest with decreased protein expression of CDC25C, CDK1, cyclin B1 and p21 were observed in Hep3B cells treated with flavonoids of C. platymamma. Further, protein expression of Bcl-XL, Bax, caspase-3 and -9 were also modulated by C. platymamma flavonoids treatment indicating that cell death is through intrinsic apoptotic pathway. Moreover, C. platymamma flavonoids also regulated the phosphorylation of MAPKs, PI3K, and Akt in Hep3B cells. Relevant to inhibiting metastasis, C. platymamma treatment reduced wound closure of Hep3B cells and the protein expression of matrix metalloproteinase-2 and -9 were reduced in C. platymamma treated cells. The results show that C. platymamma flavonoids induce cell cycle arrest and apoptosis following activation of MAPKs and suppression of PI3K/Akt pathway which eventually inhibits cell migration in Hep3B cells. The finding provides evidence on biochemical activities of C. platymamma Hort.et Tanaka, which would be an essential agent for hepatocellular carcinoma (HCC) treatment.

  6. A fully liquid DTaP-IPV-Hep B-PRP-T hexavalent vaccine for primary and booster vaccination of healthy Mexican children.

    Science.gov (United States)

    Aquino, Amalia Guadalupe Becerra; Brito, Maricruz Gutiérrez; Doniz, Carlos E Aranza; Herrera, Juan Francisco Galán; Macias, Mercedes; Zambrano, Betzana; Plennevaux, Eric; Santos-Lima, Eduardo

    2012-10-05

    To evaluate an investigational, fully liquid hexavalent diphtheria-tetanus-acellular pertussis-inactivated poliovirus-hepatitis B-Haemophilus influenzae type b (DTaP-IPV-Hep B-PRP-T: Hexaxim™) vaccine for primary and booster vaccination of healthy children in Mexico. Infants (N=1189) were randomized to receive one of three lots of the DTaP-IPV-Hep B-PRP-T vaccine or a licensed hexavalent control vaccine (Infanrix™ hexa) for primary vaccination at 2, 4 and 6 months. All participants who completed the primary series and agreed to participate in the booster part of the study received a dose of the investigational vaccine at 15-18 months of age. Validated serological assays and parental reports were used to assess immunogenicity and safety, respectively. Post-primary vaccination, ≥95.8% of participants in both the DTaP-IPV-Hep B-PRP-T and control groups were seroprotected (SP) against diphtheria, tetanus, poliovirus, hepatitis B and PRP, or had seroconverted (SC) to the pertussis toxin (PT) and filamentous hemagglutinin (FHA) pertussis antigens. The SP/SC rates induced by the three DTaP-IPV-Hep B-PRP-T lots were equivalent. No differences in SP/SC rates were observed between the pooled lots of investigational vaccine and the control vaccine. Antibody persistence at 15-18 months was comparable between groups, with strong increases in all antibody concentrations post-DTaP-IPV-Hep B-PRP-T booster. Both vaccines were well tolerated for primary vaccination, as was the booster dose of DTaP-IPV-Hep B-PRP-T. These study findings confirm the suitability of the combined, fully liquid DTaP-IPV-Hep B-PRP-T vaccine for inclusion in routine childhood vaccination schedules. Copyright © 2012 Elsevier Ltd. All rights reserved.

  7. Animal welfare: concepts and practical procedures to evaluate the swine productions systems

    Directory of Open Access Journals (Sweden)

    Xavier Manteca

    2014-02-01

    Full Text Available The concepts of animal welfare have received a wide discussion by international scientific community during the last decades. The most accepted definition consider the animal welfare like a multidimensional theme, where are involved the emotion, the biological activity and the natural behavior. However, considering the clearness of this concepts, another challenge is presented, where is questioned how we can evaluate the animal welfare in commercial systems. Thus, the Welfare Quality® project gave the understanding about the welfare subjects and how we can to evaluate its in commercial farms and slaughterhouses. Then the main of this article is present the principal concepts and practical means to evaluate the animal welfare in swine, according the Welfare Quality® protocols, with the identifications of the positive and critical aspects involved.

  8. Dosimetric evaluation in panoramic and tele-radiography procedures; Avaliacao dosimetrica em procedimentos de radiografia panoramica e teleradiografias

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Georgge Gomes

    2004-07-01

    The present work had as an objective to evaluate the skin surface entrance dose in panoramic and tele radiography procedures in three clinics in Recife - Pernambuco - Brazil, and to contribute with data for the determination of reference levels for super cited extra oral procedures, for this purpose, operational conditions in 3 clinics were evaluated in Recife, aiming to evaluate the existence and integrity of the radioprotection equipment, manner and conditions of image processing; and radiographic equipment parameters such as the dimension of the irradiation filed, the total filtration, the exposure time and the potential applied to the X ray tube. For an estimation of the skin entrance dose of the patient, the phantom Randon Alderson and thermoluminescence dosemeters were used. From these values and the conversion factors determined by the Monte Carlo technique, with the phantom MAX it was possible to estimate the dose absorbed in the organ due to the tele radiography procedures. Regarding panoramic radiography the study showed that the more elevated doses occurred in the parotid gland region which is near rotational venters. In the case of tele radiography the highest dose value occurred in the regions corresponding to the temporal lobe of the brain, followed by linfonodes, ears and parotid glands. The doses absorbed in the eyes and the thyroid gland were, 0.037 mGy and 0.002 mGy in Clinic A and 0.062 mGy and 0.003 mGy in Clinic C, respectively. Regarding equipment test, inadequacy was found in the beam collimation in Clinic A and in the reproducibility of the X ray exposure in Clinic C. The total filtration in both clinics was inadequate.(author)

  9. INSPIRE: A new scientific information system for HEP

    Science.gov (United States)

    Ivanov, R.; Raae, L.

    2010-04-01

    The status of high-energy physics (HEP) information systems has been jointly analyzed by the libraries of CERN, DESY, Fermilab and SLAC. As a result, the four laboratories have started the INSPIRE project - a new platform built by moving the successful SPIRES features and content, curated at DESY, Fermilab and SLAC, into the open-source CDS Invenio digital library software that was developed at CERN. INSPIRE will integrate current acquisition workflows and databases to host the entire body of the HEP literature (about one million records), aiming to become the reference HEP scientific information platform worldwide. It will provide users with fast access to full text journal articles and preprints, but also material such as conference slides and multimedia. INSPIRE will empower scientists with new tools to discover and access the results most relevant to their research, enable novel text- and data-mining applications, and deploy new metrics to assess the impact of articles and authors. In addition, it will introduce the "Web 2.0" paradigm of user-enriched content in the domain of sciences, with community-based approaches to scientific publishing. INSPIRE represents a natural evolution of scholarly communication built on successful community-based information systems, and it provides a vision for information management in other fields of science. Inspired by the needs of HEP, we hope that the INSPIRE project will be inspiring for other communities.

  10. A community Q&A for HEP Software and Computing ?

    CERN Multimedia

    CERN. Geneva

    2016-01-01

    How often do you use StackOverflow or ServerFault to find information in your daily work? Would you be interested in a community Q&A site for HEP Software and Computing, for instance a dedicated StackExchange site? I looked into this question...

  11. I - Multivariate Classification and Machine Learning in HEP

    CERN Multimedia

    CERN. Geneva

    2016-01-01

    Traditional multivariate methods for classification (Stochastic Gradient Boosted Decision Trees and Multi-Layer Perceptrons) are explained in theory and practise using examples from HEP. General aspects of multivariate classification are discussed, in particular different regularisation techniques. Afterwards, data-driven techniques are introduced and compared to MC-based methods.

  12. Involvement of mitochondrial pathway in NCTD-induced cytotoxicity in human hepG2 cells

    Directory of Open Access Journals (Sweden)

    Liu Shi-quan

    2010-11-01

    Full Text Available Abstract Background Norcantharidin, the demethylated analog of cantharidin derived from a traditional Chinese medicine, Mylabris, has been used in the treatment of anti-cancer effects. However, the detailed mechanisms underlying this process are generally unclear. The aim of this study was to investigate the mechanism of NCTD-induced apoptosis in HepG2 cells. Methods The cytotoxicity was measured by MTT assay for cellular viability and by flow cytometry. The mitochondrial membrane potential and reactive oxygen species production was evaluated by flow cytometry analysis. The role of caspase activities were assayed using caspase apoptosis detection kit . Western blot analysis was used to evaluate the level of Cyto-C, Bcl-2, Bax, Bid, caspase 3, -9, -8 and PARP expression Results After treatment with NCTD, a decrease in the viability of HepG2 cells and increase in apoptosis were observed. NCTD-induced apoptosis was accompanied by an increase in ROS production, loss of mitochondrial membrane potential and release of cytochrome c(cyto-c from the mitochondria to the cytosol and down-regulation of anti-apoptotic protein Bcl-2 levels with concurrent up-regulation in pro-apoptotic protein Bax levels. However, another pro-apoptotic molecule, Bid, showed no change in such same treatment. NCTD-increased activity of caspase 9,caspase 3 and the subsequent cleavage caspase substrate PARP were also observed. The expression levels of pro-caspase-8 were not changed after NCTD treatment. Conclusion These results indicate that NCTD induced cytotoxicity in HepG2 cells by apoptosis, which is mediated through ROS generation and mitochondrial pathway.

  13. Pilot-In-The-Loop Evaluation of the Approach Procedures Expert System (APES)

    National Research Council Canada - National Science Library

    Toms, Mona

    1997-01-01

    ...), to support the pilot's use of electronic approach plates in flying instrument approaches. This report describes the APES decision aid and the methodology and results of the Vehicle-Pilot Integration Branch's pilot-in-the-loop evaluation of the APES...

  14. From Equivalence of Degrees to Evaluation of Competence. Present Procedures and Practices, New Avenues.

    Science.gov (United States)

    Guiton, Jean

    The problem of comparing studies and degrees and diplomas completed in different countries and problems that arise in connection with the comparability of studies and the evaluation of competence are examined, and new solutions are suggested. A comprehensive analysis must consider the concerns and needs of the users, including students,…

  15. An Analysis of Evaluation Procedures, Disability Types, and Recommended Treatments for 100 Deaf-Blind Children.

    Science.gov (United States)

    Curtis, W. Scott; Donlon, Edward T.

    Descriptions by various professionals of 70 multiply handicapped deaf-blind children evaluated at the Syracuse University Center for the Development of Blind Children were examined for terminology used to describe the child and his life situation. Purposes were to develop some epidemiological characteristics of the multiply handicapped, deaf-blind…

  16. A data collection and processing procedure for evaluating a research program

    Science.gov (United States)

    Giuseppe Rensi; H. Dean Claxton

    1972-01-01

    A set of computer programs compiled for the information processing requirements of a model for evaluating research proposals are described. The programs serve to assemble and store information, periodically update it, and convert it to a form usable for decision-making. Guides for collecting and coding data are explained. The data-processing options available and...

  17. Screening for Specific Language Impairment in Preschool Children: Evaluating a Screening Procedure Including the Token Test

    Science.gov (United States)

    Willinger, Ulrike; Schmoeger, Michaela; Deckert, Matthias; Eisenwort, Brigitte; Loader, Benjamin; Hofmair, Annemarie; Auff, Eduard

    2017-01-01

    Specific language impairment (SLI) comprises impairments in receptive and/or expressive language. Aim of this study was to evaluate a screening for SLI. 61 children with SLI (SLI-children, age-range 4-6 years) and 61 matched typically developing controls were tested for receptive language ability (Token Test-TT) and for intelligence (Wechsler…

  18. The Dutch Review Process for Evaluating the Quality of Psychological Tests: History, Procedure, and Results

    Science.gov (United States)

    Evers, Arne; Sijtsma, Klaas; Lucassen, Wouter; Meijer, Rob R.

    2010-01-01

    This article describes the 2009 revision of the Dutch Rating System for Test Quality and presents the results of test ratings from almost 30 years. The rating system evaluates the quality of a test on seven criteria: theoretical basis, quality of the testing materials, comprehensiveness of the manual, norms, reliability, construct validity, and…

  19. Educational quality in nursery schools. From procedures for evaluation to in-service training.

    Directory of Open Access Journals (Sweden)

    Nicoletta Calzolari

    2010-07-01

    Full Text Available This paper describes a training-research project promoted by the Educational Coordination of Bologna Municipality and involving 15 Kindergarten sections in a district of Bologna city. The research was conducted in the school year 2009-2010. The project was planned as an Evaluation Research oriented to introduce some Quality Evaluation proceedings into the Kindergarten and to promote, into the group of the teachers that have been involved, some processes of in-service training and educational planning. The Scale for Observation and Evaluation of Kindergarten SOVASI has been used following the methodological suggestions given by M. Ferrari (1994 as a reference and an opportunity for critical reflexion on the scale itself. After a brief report of the theoretical framework of the research, the authors present an analysis of the results concerning the Quality Evaluation of the Kindergartens, some critical reflexions on the proceedings and on the tool that has been used and the ways the researches conducted the data restitution meetings with the teachers in order to promote their professionalism.

  20. A Comparative Evaluation of Procedural Level Generators in the Mario AI Framework

    DEFF Research Database (Denmark)

    Horn, Britton; Dahlskog, Steve; Shaker, Noor

    dierent from another in terms of its output. To remedy this, we have conducted a large-scale comparative evaluation of level generators for the Mario AI Benchmark, a research-friendly clone of the classic platform game Super Mario Bros. In all, we compare the output of seven dierent level generators from...

  1. Biofilm characteristics and evaluation of the sanitation procedures of thermophilic Aeribacillus pallidus E334 biofilms.

    Science.gov (United States)

    Kilic, Tugba; Karaca, Basar; Ozel, Beste Piril; Ozcan, Birgul; Cokmus, Cumhur; Coleri Cihan, Arzu

    2017-04-01

    The ability of Aeribacillus pallidus E334 to produce pellicle and form a biofilm was studied. Optimal biofilm formation occurred at 60 °C, pH 7.5 and 1.5% NaCl. Extra polymeric substances (EPS) were composed of proteins and eDNA (21.4 kb). E334 formed biofilm on many surfaces, but mostly preferred polypropylene and glass. Using CLSM analysis, the network-like structure of the EPS was observed. The A. pallidus biofilm had a novel eDNA content. DNaseI susceptibility (86.8% removal) of eDNA revealed its importance in mature biofilms, but the purified eDNA was resistant to DNaseI, probably due to its extended folding outside the matrix. Among 15 cleaning agents, biofilms could be removed with alkaline protease and sodium dodecyl sulphate (SDS). The removal of cells from polypropylene and biomass on glass was achieved with combined SDS/alkaline protease treatment. Strong A. pallidus biofilms could cause risks for industrial processes and abiotic surfaces must be taken into consideration in terms of sanitation procedures.

  2. [Cutaneous malignant melanoma; diagnostic procedures and their evaluation in diagnosing and mapping sentinel nodes].

    Science.gov (United States)

    Valsamaki, Pipitsa; Zanglis, Antonios; Gerali, Sophia

    2009-01-01

    Cutaneous malignant melanoma represents 4% of all the malignant neoplasms. Nevertheless, 79% of the fatal malignant skin diseases are attributed to melanoma. In melanoma patients, the most important prognostic factor is the regional lymph node invasion. The present article describes the classical diagnostic, staging and therapeutic strategies in melanoma patients. In the realm of cutaneous melanoma, pre-operative lymphoscintigraphy and the intra-operative gamma-probe detection, removal and biopsy of the sentinel lymph node(s), comprise convenient, procedures, virtually lacking morbidity. The definition of sentinel includes the first lymph node or nodes, draining the lymph from the primary lesion, thereby attributed with the highest probability of harbouring migrating metastatic cells. A sentinel lymph node negative for malignancy accurately "predicts" the absence of melanoma cell invasion in all the rest regional and distant lymphatic basins. The method aims in the best discrimination of high-risk patients, as candidates for selective lymphadenectomy, with or without adjunctive treatment. Radionuclide-guided lymphatic mapping and surgery is based on the phagocytosis of colloidal-based radiopharmaceuticals by the macrophages encountered in functional tumour-infiltrated sentinel nodes. The nuclear methods successfully add to the staging and the potential surgical treatment of the disease.

  3. Semiotics and semiology of Nursing: evaluation of undergraduate students' knowledge on procedures

    Directory of Open Access Journals (Sweden)

    Gabriela de Sousa Martins Melo

    Full Text Available ABSTRACT Objective: to assess the knowledge of scholars on Nursing regarding simple hands hygiene (SHH, blood pressure measurement (BP, peripheral venipuncture (PV with venous catheter and male urethral catheterization delay (UCD procedures. Method: quantitative study carried out between February and May 2014, with 186 undergraduate Nursing students from 5th to 9th period of a public university of Rio Grande do Norte, with application of four questionnaires. One carried out descriptive and analytic analysis. Results: the students presented low average percentage of right answers, especially in blood pressure measurement (55.5%; SHH's average was higher than 70%. The average of correct answers was the highest in SHH (8.6, followed by UCD (7.8, PV (7.4 and BP (6.7. The questions regarding the topic "concepts" showed less correct answers when comparing it to the topic "technique steps". Conclusion: it is necessary to establish knowledge monitoring strategies, in order to stimulate the constant improvement.

  4. An evaluation of the fixed concentration procedure for assessment of acute inhalation toxicity.

    Science.gov (United States)

    Sewell, Fiona; Ragan, Ian; Indans, Ian; Marczylo, Tim; Stallard, Nigel; Griffiths, David; Holmes, Thomas; Smith, Paul; Horgan, Graham

    2018-01-06

    Acute inhalation studies are conducted in animals as part of chemical hazard identification and for classification and labelling. Current methods employ death as an endpoint (Organisation for Economic Co-operation and Development (OECD) test guideline (TG) 403 and TG436) while the recently approved fixed concentration procedure (FCP) (OECD TG433) uses fewer animals and replaces lethality as an endpoint with evident toxicity. Evident toxicity is the presence of clinical signs that predict that exposure to the next highest concentration will cause severe toxicity or death in most animals. Approval of TG433 was the result of an international initiative, led by the National Centre for the Replacement, Refinement & Reduction of Animals in Research (NC3Rs), which collected data from six laboratories on clinical signs recorded for inhalation studies on 172 substances. This paper summarises previously published data and describes the additional analyses of the dataset that were essential for approval of the TG. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

  5. Evaluation of designer amphetamine interference in GC-MS amine confirmation procedures.

    Science.gov (United States)

    Holler, Justin M; Vorce, Shawn P; Knittel, Jessica L; Malik-Wolf, Brittany; Levine, Barry; Bosy, Thomas Z

    2014-06-01

    In recent years, a class of new designer drugs commonly referred to as 'bath salts' have made their way to the illicit drug market. The most common drugs encountered are designer amphetamines and cathinones. Many analytical methods for analysis and identification of bath salts have been published, but there has been little reported on their impact on existing gas chromatography-mass spectrometry (GC-MS) amine confirmation methods. Due to structural similarities, the potential exists that designer amphetamines may interfere with methods used for analysis of sympathomimetic amines. Methiopropamine, 4-fluoroamphetamine, 4-fluoromethamphetamine (4-FMA) and 4-methylamphetamine were examined for potential interference with immunoassays and GC-MS confirmation analysis utilizing three derivatization procedures: R(-)-α-methoxy-α-trifluoromethylphenylacetyl chloride (R-MTPAC), heptafluorobutyric anhydride (HFBA) and chlorodifluoroacetic anhydride (ClF(2)AA). Significant cross-reactivity was observed with all the four compounds on the Syva Emit(®) II Plus Amphetamines and Roche KIMS Amphetamines II immunoassays. Laboratories utilizing GC-MS selected-ion-monitoring confirmation methods with R-MTPAC, HFBA or ClF(2)AA derivatives could experience potential chromatographic and mass spectral interferences from 4-fluroamphetamine, 4-FMA and methiopropamine in the form of ion ratio and quantitative failures. Careful ion selection, proper selectivity and specificity studies during method validation and rigid chromatographic and spectral acceptance criteria are required to assure the robustness and accuracy of GC-MS methods.

  6. Concept formation based on value relations evaluated with a matching-to-sample procedure.

    Science.gov (United States)

    Pérez-González, Luis Antonio

    2008-01-01

    To study concept formation based on relations, adults were taught and tested on complex discriminations involving figures that varied in colors, forms, and orientations. In Experiment 1, participants learned to select figures with values A1 and B1 or values B1 and C1; thereafter, they consistently selected figures with values A1 and C1. Selections were based on the relations among the values, rather than on perceptual properties. Experiments 2 and 3 studied generalization with a matching-to-sample procedure: participants learned to select "yes" in the presence of the positive figures, such as A1B1, and "no" in the presence of the negative figures. Thereafter, all figures that resulted from combining three values of the three relevant dimensions were probed. Participants typically selected "yes" in the presence of the novel figures that had two or three values related to one another and selected "no" in the presence of the other figures. Finally, two participants learned a simple discrimination. They did not generalize responding to other figures with the same values; instead, their performance in the generalization test remained almost unaltered. Thus, the concept based on relations was not affected by the simple discrimination. These results showed some unique properties of the concept based on relations and challenge previous theories on concept formation.

  7. Evaluation of the results and complications of the Latarjet procedure for recurrent anterior dislocation of the shoulder.

    Science.gov (United States)

    da Silva, Luciana Andrade; da Costa Lima, Álvaro Gonçalves; Kautsky, Raul Meyer; Santos, Pedro Doneux; do Val Sella, Guilherme; Checchia, Sergio Luiz

    2015-01-01

    Evaluate the results and complications of Latarjet procedure in patients with anterior recurrent dislocation of the shoulder. Fifty-one patients (52 shoulders) with anterior recurrent dislocation, surgically treated by Latarjet procedure, were analyzed retrospectively. The average follow-up time was 22 months, range 12-66 months; The age range was 15-59 years with a mean of 31; regarding sex, 42 (82.4%) patients were male and nine (17.6%) were female. The dominant side was affected in 29 (55.8%) shoulders. Regarding the etiology, 48 (92.3%) reported trauma and four (7.6%) had the first episode after a convulsion. The average elevation, lateral rotation and medial rotation of the operated shoulder were, respectively, 146° (60-80°), 59° (0-85°) and T8 (T5 gluteus), with statistical significance for decreased range of motion in all planes, compared with the other side. The scores of Rowe and UCLA were 90.6 and 31.4, respectively, in the postoperative period. Eleven shoulders (21.2%) had poor results: signs of instability (13.4%), non-union (11.5%) and early loosening of the synthesis material (1.9%). There was a correlation between poor results and convulsive patients (p = 0.026). We conclude that the Latarjet procedure for correction of anterior recurrent dislocation leads to good and excellent results in 82.7% of cases. Complications are related to errors in technique.

  8. Evaluation of the Trends, Concomitant Procedures, and Complications With Open and Arthroscopic Rotator Cuff Repairs in the Medicare Population.

    Science.gov (United States)

    Jensen, Andrew R; Cha, Peter S; Devana, Sai K; Ishmael, Chad; Di Pauli von Treuheim, Theo; D'Oro, Anthony; Wang, Jeffrey C; McAllister, David R; Petrigliano, Frank A

    2017-10-01

    Medicare insures the largest population of patients at risk for rotator cuff tears in the United States. To evaluate the trends in incidence, concomitant procedures, and complications with open and arthroscopic rotator cuff repairs in Medicare patients. Cohort study; Level of evidence, 3. All Medicare patients who had undergone open or arthroscopic rotator cuff repair from 2005 through 2011 were identified with a claims database. Annual incidence, concomitant procedures, and postoperative complications were compared between these 2 groups. In total, 372,109 rotator cuff repairs were analyzed. The incidence of open repairs decreased (from 6.0 to 4.3 per 10,000 patients, P arthroscopic repairs increased (from 4.5 to 7.8 per 10,000 patients, P arthroscopic group were more likely to have undergone concomitant subacromial decompression than those in the open group (87% vs 35%, P arthroscopic, P arthroscopic rotator cuff repairs have increased in incidence and now represent the majority of rotator cuff repair surgery. Among concomitant procedures, subacromial decompression was most commonly performed despite evidence suggesting a lack of efficacy. Infections and stiffness were rare complications that were slightly but significantly more frequent in open rotator cuff repairs.

  9. Evaluation of gigabit links for use in HEP trigger processing

    CERN Document Server

    Anderson, C R

    1999-01-01

    commercial OPNET package The next generation of colliders will take experimental particle physics into energy regimes where the potential for fundamental new discoveries is overshadowed by the immense technological challenges that have to be met in building the necessary detectors. One major technological challenge is to build detectors with fine granularity that can withstand the very high levels of radiation around the interaction region. The final challenge is to build and operate the high speed electronics that can readout and process the huge volumes of data that will be generated. In particular, a key demand is that efficient triggers be built that will filter out as much of the background as possible at as early a stage as possible, without losing or biasing the tiny physics signal. These triggers must be fast and affordable. This thesis is concerned with one aspect of this scenario: how to provide a low cost but very fast switching system that can direct the streams of data coming from the detector in...

  10. Evaluation of a romifidine constant rate infusion protocol with or without butorphanol for dentistry and ophthalmologic procedures in standing horses.

    Science.gov (United States)

    Marly, Charlotte; Bettschart-Wolfensberger, Regula; Nussbaumer, Paeivi; Moine, Sebastien; Ringer, Simone K

    2014-09-01

    To compare the clinical usefulness of constant rate infusion (CRI) protocols of romifidine with or without butorphanol for sedation of horses. Prospective 'blinded' controlled trial using block randomization. Forty healthy Freiberger stallions. The horses received either intravenous (IV) romifidine (loading dose: 80 μg kg(-1) ; infusion: 30 μg kg(-1)  hour(-1) ) (treatment R, n = 20) or romifidine combined with butorphanol (romifidine loading: 80 μg kg(-1) ; infusion: 29 μg kg(-1)  hour(-1) , and butorphanol loading: 18 μg kg(-1) ; infusion: 25 μg kg(-1)  hour(-1) ) (treatment RB, n = 20). Twenty-one horses underwent dentistry and ophthalmic procedures, while 19 horses underwent only ophthalmologic procedure and buccal examination. During the procedure, physiologic parameters and occurrence of head/muzzle shaking or twitching and forward movement were recorded. Whenever sedation was insufficient, additional romifidine (20 μg kg(-1) ) was administered IV. Recovery time was evaluated by assessing head height above ground. At the end of the procedure, overall quality of sedation for the procedure was scored by the dentist and anaesthetist using a visual analogue scale. Statistical analyses used two-way anova or linear mixed models as relevant. Sedation quality scores as assessed by the anaesthetist were R: median 7.55, range: 4.9-9.0 cm, RB: 8.8, 4.7-10.0 cm, and by the dentist R: 6.6, 3.0-8.2 cm, RB: 7.9, 6.6-8.8 cm. Horses receiving RB showed clinically more effective sedation as demonstrated by fewer poor scores and a tendency to reduced additional drug requirements. More horses showed forward movement and head shaking in treatment RB than treatment R. Three horses (two RB, one R) had symptoms of colic following sedation. The described protocols provide effective sedation under clinical conditions but for dentistry procedures, the addition of butorphanol is advantageous. © 2014 Association of Veterinary Anaesthetists and the

  11. Stable expression of human muscle-specific kinase in HEp-2 M4 cells for automatic immunofluorescence diagnostics of myasthenia gravis.

    Science.gov (United States)

    George, Sandra; Paulick, Silvia; Knütter, Ilka; Röber, Nadja; Hiemann, Rico; Roggenbuck, Dirk; Conrad, Karsten; Küpper, Jan-Heiner

    2014-01-01

    Muscle-specific kinase (MuSK) belongs to the nicotinic acetylcholine receptor complex which is targeted by pathogenic autoantibodies causing Myasthenia gravis. While up to 95% of patients with generalized Myasthenia gravis were shown to be positive for acetylcholine receptor-specific autoantibodies, up to 70% of the remaining patients develop autoantibodies against MuSK. Discrimination of the autoantibody specificity is important for therapy of Myasthenia gravis. Recently, the new automatic fluorescence assessment platform AKLIDES has been developed for immunofluorescence-based diagnostics of autoimmune diseases. In order to establish an AKLIDES procedure for the detection of MuSK-specific autoantibodies (anti-MuSK), we developed a recombinant HEp-2 cell clone expressing the human MuSK cDNA. Here we show at the mRNA and protein level that the cell clone HEp-2 M4 stably expresses human MuSK. We provide evidence for a localization of MuSK at the cell membrane. Using cell clone HEp-2 M4 on the AKLIDES system, we investigated 34 patient sera that were previously tested anti-MuSK positive by radioimmunoassay as positive controls. As negative controls, we tested 29 acetylcholine receptor-positive but MuSK-negative patient sera, 30 amytrophic lateral sclerosis (ALS) patient sera and 45 blood donors. HEp-2 M4 cells revealed a high specificity for the detection of MuSK autoantibodies from 25 patient sera assessed by a specific pattern on HEp-2 M4 cells. By using appropriate cell culture additives, the fraction of cells stained positive with anti-MuSK containing sera can be increased from 2-16% to 10-48%, depending on the serum. In conclusion, we provide data showing that the novel recombinant cell line HEp-2 M4 can be used to screen for anti-MuSK with the automatic AKLIDES system.

  12. Stable expression of human muscle-specific kinase in HEp-2 M4 cells for automatic immunofluorescence diagnostics of myasthenia gravis.

    Directory of Open Access Journals (Sweden)

    Sandra George

    Full Text Available Muscle-specific kinase (MuSK belongs to the nicotinic acetylcholine receptor complex which is targeted by pathogenic autoantibodies causing Myasthenia gravis. While up to 95% of patients with generalized Myasthenia gravis were shown to be positive for acetylcholine receptor-specific autoantibodies, up to 70% of the remaining patients develop autoantibodies against MuSK. Discrimination of the autoantibody specificity is important for therapy of Myasthenia gravis. Recently, the new automatic fluorescence assessment platform AKLIDES has been developed for immunofluorescence-based diagnostics of autoimmune diseases. In order to establish an AKLIDES procedure for the detection of MuSK-specific autoantibodies (anti-MuSK, we developed a recombinant HEp-2 cell clone expressing the human MuSK cDNA. Here we show at the mRNA and protein level that the cell clone HEp-2 M4 stably expresses human MuSK. We provide evidence for a localization of MuSK at the cell membrane. Using cell clone HEp-2 M4 on the AKLIDES system, we investigated 34 patient sera that were previously tested anti-MuSK positive by radioimmunoassay as positive controls. As negative controls, we tested 29 acetylcholine receptor-positive but MuSK-negative patient sera, 30 amytrophic lateral sclerosis (ALS patient sera and 45 blood donors. HEp-2 M4 cells revealed a high specificity for the detection of MuSK autoantibodies from 25 patient sera assessed by a specific pattern on HEp-2 M4 cells. By using appropriate cell culture additives, the fraction of cells stained positive with anti-MuSK containing sera can be increased from 2-16% to 10-48%, depending on the serum. In conclusion, we provide data showing that the novel recombinant cell line HEp-2 M4 can be used to screen for anti-MuSK with the automatic AKLIDES system.

  13. Interim radiological safety standards and evaluation procedures for subseabed high-level waste disposal

    Energy Technology Data Exchange (ETDEWEB)

    Klett, R.D.

    1997-06-01

    The Seabed Disposal Project (SDP) was evaluating the technical feasibility of high-level nuclear waste disposal in deep ocean sediments. Working standards were needed for risk assessments, evaluation of alternative designs, sensitivity studies, and conceptual design guidelines. This report completes a three part program to develop radiological standards for the feasibility phase of the SDP. The characteristics of subseabed disposal and how they affect the selection of standards are discussed. General radiological protection standards are reviewed, along with some new methods, and a systematic approach to developing standards is presented. The selected interim radiological standards for the SDP and the reasons for their selection are given. These standards have no legal or regulatory status and will be replaced or modified by regulatory agencies if subseabed disposal is implemented. 56 refs., 29 figs., 15 tabs.

  14. Elimination of volatile chemicals in disinfectant evaluation procedures by freeze drying.

    Science.gov (United States)

    Quinn, P J; Scanlon, M P

    2000-09-01

    Standard methods for the removal or inactivation of disinfectants from reaction mixtures are often unsatisfactory when dealing with high concentrations of particulate organic matter. A method employing freeze drying was developed to eliminate residual disinfectant activity from reaction mixtures. Following the addition of volatile chemicals to sterilized cattle slurry, samples were removed from the mixture, freeze dried, and the dried material was reconstituted with diluent containing Escherichia coli as the test micro-organism to determine if the volatile chemicals had been removed. Twelve chemicals and one commercial disinfectant (Virkon S) were evaluated at different concentrations. The chemicals selected were acetone, ammonium hydroxide, carbon tetrachloride, chloroform, dichloromethane, ethyl alcohol, formalin, hydrogen peroxide, isopropyl alcohol, peracetic acid, sodium hypochlorite and xylene. Eight chemicals, acetone, ammonium hydroxide, carbon tetrachloride, chloroform, dichloromethane, ethyl alcohol, isopropyl alcohol and xylene were removed by freeze drying. The remaining four chemicals and the commercial disinfectant, which were not removed by freeze drying, could not be evaluated by this method.

  15. A Bayesian Procedure for Resource Evaluation of Petroleum Provinces in the Early Stages of Exploration

    OpenAIRE

    Medova, E.

    1981-01-01

    A major open problem in quantitative methods for petroleum resource evaluation concerns the provision of statistical techniques for geological provinces in the early stages of exploration-for example, if all exploratory wells drilled to date have been found to be dry or if only a few fields have been discovered. A considerable body of literature exists concerning statistical methods for mature provinces in which discovery volumes are on a general declining trend; all these methods use explora...

  16. Application of Toxicity Identification and Evaluation Procedures for Dredged Material Management

    Science.gov (United States)

    2017-02-01

    complex . The evaluation of environmental impacts from these alternative management strategies typically requires more data than the assessment of...analysis • Enhance phototoxicity • Preferentially extract and dose test organisms • Measure toxicant in organism • Measure DNA damage [8,10-15...toxicity [16-20] Cationic Metals • Cadmium • Chromium • Copper • Iron • Lead • Silver • Zinc • SIR-300 resin • EDTA • CM resin • Reduce

  17. Evaluation of relevant information for optimal reflector modeling through data assimilation procedures

    OpenAIRE

    Argaud Jean-Philippe; Bouriquet Bertrand; Clerc Thomas; Lucet-Sanchez Flora; Ponçot Angélique

    2015-01-01

    The goal of this study is to look after the amount of information that is mandatory to get a relevant parameters optimisation by data assimilation for physical models in neutronic diffusion calculations, and to determine what is the best information to reach the optimum of accuracy at the cheapest cost. To evaluate the quality of the optimisation, we study the covariance matrix that represents the accuracy of the optimised parameter. This matrix is a classical output of the data assimilation ...

  18. Running-based Anaerobic Sprint Test as a Procedure to Evaluate Anaerobic Power.

    Science.gov (United States)

    Andrade, V L; Zagatto, A M; Kalva-Filho, C A; Mendes, O C; Gobatto, C A; Campos, E Z; Papoti, M

    2015-12-01

    The aim of this study was to evaluate the use of the running anaerobic sprint test (RAST) as a predictor of anaerobic capacity, compare it to the maximal accumulated oxygen deficit (MAOD) and to compare the RAST's parameters with the parameters of 30-s all-out tethered running on a treadmill. 39 (17.0±1.4 years) soccer players participated in this study. The participants underwent an incremental test, 10 submaximal efforts [50-95% of velocity correspondent to VO(2MAX) (vVO(2MAX))] and one supramaximal effort at 110% of vVO(2MAX) for the determination of MAOD. Furthermore, the athletes performed the RAST. In the second stage the 30-s all-out tethered running was performed on a treadmill (30-s all-out), and compared with RAST. No significant correlation was observed between MAOD and RAST parameters. However, significant correlations were found between the power of the fifth effort (P5) of RAST with peak and mean power of 30-s all-out (r=0.73 and 0.50; pRAST do not have an association with MAOD, suggesting that this method should not be used to evaluate anaerobic capacity. Although the correlations between RAST parameters with 30-s all-out do reinforce the RAST as an evaluation method of anaerobic metabolism, such as anaerobic power. © Georg Thieme Verlag KG Stuttgart · New York.

  19. Interactions between delta 9-tetrahydrocannabinol and cannabidiol as evaluated by drug discrimination procedures in rats and pigeons.

    Science.gov (United States)

    Hiltunen, A J; Järbe, T U

    1986-02-01

    Animals (rats and pigeons) were trained to discriminate between the presence and absence of delta 9-THC; the training doses were, respectively: 0.56 mg/kg (pigeons) and 3.0 mg/kg (rats). Once the drug discrimination was mastered, the pigeons were tested repeatedly after a single intramuscular (i.m.) injection of delta 9-THC (0.56 mg/kg) at the following intervals 0.5, 1.5, 4.5 and 9 hr after the injection. These results were compared with data from a separate procedure, i.e. where the various intervals after injection were examined only once per injection and both procedures yielded essentially the same outcome. Thus, less than 50% appropriate responding to THC was observed at 0.5 and 9 hr after injection, whereas greater than 90% responding to THC occurred at 1.5 and 4.5 hr. The two procedures have previously been compared in rats (Järbe, Swedberg and Mechoulam, 1981). The repeated tests procedure was then used to evaluate combinations of delta 9-THC and cannabidiol in both species. Cannabidiol prolonged the cue effects of 1 mg/kg of delta 9-THC (intraperitoneal route of administration) in rats but did not change the time-effect curve for delta 9-THC in pigeons (dose range examined: 0.10--0.56 mg/kg); the challenge doses of cannabidiol were, respectively: 30.0 mg/kg (i.p.) and 17.5 mg/kg (i.m.). The rate of responding did not differ in tests with combinations of delta 9-THC and cannabidiol as compared to delta 9-THC given alone in pigeons. Subcutaneously administered 3-PPP, a dopamine pre-synaptic blocker, did not induce responding appropriate for delta 9-THC in rats.

  20. Evaluation of tensile strength and surface topography of orthodontic wires after infection control procedures: An in vitro study

    Directory of Open Access Journals (Sweden)

    M Brindha

    2014-01-01

    Full Text Available Aim: The aim of this study is to evaluate, the influence of four types of sterilization/disinfection procedures (autoclave, hot air oven, glutaraldehyde, and ultraviolet [UV] light on the tensile strength and surface topography of three orthodontic wires (stainless steel (SS, titanium - molybdenum alloy [TMA], and cobalt chromium (CoCr. Materials and Methods: Sample comprised of three types of 8 inches straight length segments of orthodontic wires. They were divided into three groups according to wire composition comprising of 50 samples each. Totally 50 samples of each group were then equally divided into five subgroups according to sterilization method. After sterilization and disinfection of the experimental group, surface topography was examined with scanning electron microscope (SEM and tensile strength was tested using universal testing machine. Result: The results of this study show that the mean ultimate tensile strength (UTS of SS wire after four sterilization procedures were similar to the control group (1845.815 ± 142.29 MPa.The mean UTS of TMA wire increases after four sterilization procedures when compared with the control group (874.107 ± 275.939 MPa. The mean UTS of CoCr wire remains same after UV light disinfection, but increases after other three sterilization procedures when compared with the control group (1449.759 ± 156.586 MPa. SEM photographs of the present study shows gross increase in pitting roughness of the surface topography of all the three types of wires after four types of sterilization. Conclusion: Orthodontists who want to offer maximum safety for their patients can sterilize orthodontic wires before placement, as it does not deteriorate the tensile strength and surface roughness of the alloys.

  1. AMPT-induced monoamine depletion in humans: evaluation of two alternative [{sup 123}I]IBZM SPECT procedures

    Energy Technology Data Exchange (ETDEWEB)

    Boot, Erik [Academic Medical Centre (AMC), University of Amsterdam, Department of Psychiatry, Amsterdam (Netherlands); De Bruggen, Centre for People with Intellectual Disability, Zwammerdam (Netherlands); Booij, Jan [AMC, Department of Nuclear Medicine, Amsterdam (Netherlands); Hasler, Gregor [University Hospital, Department of Psychiatry, Zuerich (Switzerland); Zinkstok, Janneke R.; Haan, Lieuwe de; Linszen, Don H.; Amelsvoort, Therese A. van [Academic Medical Centre (AMC), University of Amsterdam, Department of Psychiatry, Amsterdam (Netherlands)

    2008-07-15

    Acute monoamine depletion paradigms using alpha-methyl-para-tyrosine (AMPT) combined with single photon emission computed tomography (SPECT) have been used successfully to evaluate disturbances in central dopaminergic neurotransmission. However, severe side effects due to relatively high doses (4,500 to 8,000 mg) of AMPT have been reasons for study withdrawal. Thus, we assessed the effectiveness and tolerability of two alternative procedures, using lower doses of AMPT. Six healthy subjects underwent three measurements of striatal dopamine D{sub 2} receptor (D{sub 2}R)-binding potential (BP{sub ND}) with SPECT and the selective radiolabeled D{sub 2}R antagonist [{sup 123}I]IBZM. All subjects were scanned in the absence of pharmacological intervention (baseline) and after two different depletion procedures. In the first depletion session, over 6 h, subjects were administered 1,500 mg of AMPT before scanning. In the second depletion session, over 25 h, subjects were administered 40 mg AMPT/kg body weight. We also administered the Subjective Well-being Under Neuroleptic Treatment Scale, a self-report instrument designed to measure the subjective experience of patients on neuroleptic medication. We found no change of mean D{sub 2}R BP{sub ND} after the first and short AMPT challenge compared to the baseline. However, we found a significant increase in striatal D{sub 2}R BP{sub ND} binding after the AMPT challenge adjusted for bodyweight compared to both other regimen. Although subjective well-being worsened after the prolonged AMPT challenge, no severe side effects were reported. Our results imply a low-dosage, suitable alternative to the common AMPT procedure. The probability of side effects and study withdrawal can be reduced by this procedure. (orig.)

  2. Evaluation of assay procedures for prediction of passive transfer status in lambs.

    Science.gov (United States)

    Massimini, Genesio; Peli, Angelo; Boari, Andrea; Britti, Domenico

    2006-04-01

    To compare 4 assay procedures for prediction of passive transfer status in lambs. Thirty-one 1-day-old Sardinian lambs. Serum IgG concentration was determined by use of single radial immunodiffusion. The following were determined: serum total protein concentration as measured by refractometry (ie, refractometry serum total protein concentration), serum total protein concentration as determined by the biuret method (ie, biuret method serum total protein concentration), serum gamma-globulin concentration as determined by serum protein electrophoresis, and serum gamma-glutamyltransferase (GGT) activity as measured by spectrophotometry. Accuracy of these assays for estimation of serum IgG concentration in 1-day-old lambs was established by use of linear regression analysis. Refractometry serum total protein concentration, biuret method serum total protein concentration, and serum gamma-globulin concentration were closely and linearly correlated with serum IgG concentration. The natural logarithm (ln) of serum GGT activity was closely and linearly correlated with serum IgG concentration (ln). Refractometry serum total protein concentration, biuret method serum total protein concentration, and gamma-globulin concentration accounted for approximately 85%, 91%, and 95% of the variation in serum IgG concentration, respectively. Serum GGT activity (ln) accounted for approximately 92% of the variation in serum IgG concentration (ln). For prediction of passive transfer status in 1-day-old lambs, serum GGT activity or biuret method serum total protein concentration determination will allow for passive transfer monitoring program development. Immediate refractometry serum total protein concentration determination is beneficial in making timely management and treatment decisions. Serum gamma-globulin concentration determination can be used as a confirmatory test.

  3. Evaluation of enrichment procedures for recovering Listeria monocytogenes from dairy products.

    Science.gov (United States)

    Lammerding, A M; Doyle, M P

    1989-11-01

    Six different enrichment media and five selective plating media were compared for their suitability for the recovery of Listeria monocytogenes from dairy products. These included media used to test milk products by the U.S. Food and Drug Administration (FDA) and the U.S. Centers for Disease Control (CDC), and media developed by the U.S. Department of Agriculture (USDA) for testing meat and poultry products. Test samples included naturally contaminated goat's milk, cultured milk products and ice cream manufactured with L. monocytogenes, and unpasteurized milk inoculated with heat- and freeze-injured cells of L. monocytogenes. Generally, the media and two-stage enrichment protocol developed by the USDA, with plating of samples after two consecutive 24-h incubation periods, yielded better recoveries than all other enrichment media incubated for 24 h. A modified USDA procedure, incorporating nonselective pre-enrichment of samples by omitting acriflavine and nalidixic acid from the primary USDA enrichment broth, and transfer of a larger volume of the initial culture broth to the secondary enrichment media, significantly increased recoveries of low numbers of sublethally stressed L. monocytogenes. Prolonged incubation of samples in the FDA enrichment broth, for 7 days, did not consistently improve recoveries over the initial 24-h incubation time of the medium. The selective plating medium developed by the USDA, lithium chloride-phenylethanol-moxalactam agar, was the most effective plating agar for isolation of L. monocytogenes following enrichment of samples in any broth culture, and increased recoveries of L. monocytogenes by 19-40% compared with other selective agar media tested.

  4. Evaluation of the surface free energy of plant surfaces: toward standardizing the procedure.

    Science.gov (United States)

    Fernández, Victoria; Khayet, Mohamed

    2015-01-01

    Plant surfaces have been found to have a major chemical and physical heterogeneity and play a key protecting role against multiple stress factors. During the last decade, there is a raising interest in examining plant surface properties for the development of biomimetic materials. Contact angle measurement of different liquids is a common tool for characterizing synthetic materials, which is just beginning to be applied to plant surfaces. However, some studies performed with polymers and other materials showed that for the same surface, different surface free energy values may be obtained depending on the number and nature of the test liquids analyzed, materials' properties, and surface free energy calculation methods employed. For 3 rough and 3 rather smooth plant materials, we calculated their surface free energy using 2 or 3 test liquids and 3 different calculation methods. Regardless of the degree of surface roughness, the methods based on 2 test liquids often led to the under- or over-estimation of surface free energies as compared to the results derived from the 3-Liquids method. Given the major chemical and structural diversity of plant surfaces, it is concluded that 3 different liquids must be considered for characterizing materials of unknown physico-chemical properties, which may significantly differ in terms of polar and dispersive interactions. Since there are just few surface free energy data of plant surfaces with the aim of standardizing the calculation procedure and interpretation of the results among for instance, different species, organs, or phenological states, we suggest the use of 3 liquids and the mean surface tension values provided in this study.

  5. Clinical Evaluation of Different Pre-impression Preparation Procedures of Dental Arch.

    Science.gov (United States)

    Arora, Nitin; Arora, Monika; Gupta, Naveen; Agarwal, Manisha; Verma, Rohit; Rathod, Pankaj

    2015-07-01

    Bubbles and voids on the occlusal surface impede the actual intercuspation and pre-impression preparation aims to reduce the incidence of air bubbles and voids as well as influences the quality of occlusal reproduction and actual clinical intercuspation in the articulator. The study was undertaken to determine the influence of different pre-impression preparation procedures of antagonistic dental arch on the quality of the occlusal reproduction of the teeth in irreversible hydrocolloid impressions and to determine most reliable pre-impression preparation method to reduce the incidence of air bubbles. A total of 20 subjects were selected having full complement of mandibular teeth from second molar to second molar with well demarcated cusp height. 200 impressions were made with irreversible hydrocolloid material. The impressions were divided into five groups of 40 impressions each and each group had one specific type of pre-impression preparation. All the impressions were poured in die stone. A stereomicroscope with graduated eyepiece was used to count the number of bubbles on the occlusal surface of premolars and molars. The mean and standard deviations were calculated for each group. Mann-Whitney U-test was applied to find the significant difference between different groups. Least bubbles were found in the group in which oral cavity was dried by saliva ejector and fluid hydrocolloid was finger painted onto the occlusal surfaces immediately before the placement of impression tray in the mouth. It was found that finger painting the tooth surfaces with fluid hydrocolloid immediately before the placement of loaded impression tray in the mouth was the most reliable method. The oral cavity can be cleared more easily of excess saliva by vacuum suction rather than by use of an astringent solution.

  6. Safety and immunogenicity of an investigational fully liquid hexavalent DTaP-IPV-Hep B-PRP-T vaccine at two, four and six months of age compared with licensed vaccines in Latin America.

    Science.gov (United States)

    Macías, Mercedes; Lanata, Claudio F; Zambrano, Betzana; Gil, Ana I; Amemiya, Isabel; Mispireta, Monica; Ecker, Lucie; Santos-Lima, Eduardo

    2012-08-01

    This trial assessed the safety of a fully liquid investigational hexavalent DTaP-IPV-Hep B-PRP-T vaccine containing 10 μg Hansenula polymorpha-derived recombinant hepatitis B (hep B) antigen for primary vaccination of infants at 2, 4 and 6 months of age compared with licensed comparators. Participants received the DTaP-IPV-Hep B-PRP-T vaccine (group 1, N = 1422) or licensed DTwP-Hep B//Hib (Tritanrix-Hep B/Hib) and oral poliovirus vaccines (group 2, N = 711). The incidence of severe fever (≥ 39.6°C rectal equivalent) in the 2 groups was compared statistically; reactogenicity was evaluated from parental reports. Anti-Hep B antibody titers were measured in a subset of participants (no hepatitis B vaccination at birth) 1 month after dose 3. The investigational vaccine was well tolerated. After any dose, fever (rectal equivalent temperature ≥ 38°C) was observed in 74.8% and 92.7% of participants in groups 1 and 2; severe fever was observed in 4.0% and 5.5% of participants. Solicited injection site and systemic reactions were numerically less frequent in group 1 than group 2, although this difference was not assessed statistically. In both groups, all participants included in the immunogenicity analysis achieved anti-Hep B ≥ 10 mIU/mL and ≥ 96.2% of participants achieved anti-Hep B ≥ 100 mIU/mL, although geometric mean titer was approximately 3-fold lower for the investigational vaccine. This new, fully liquid acellular pertussis hexavalent vaccine demonstrated less reactogenicity than the licensed comparator whole cell pertussis vaccine and was highly immunogenic for the new Hep B valence.

  7. Development of Wall-Thinning Evaluation Procedure for Nuclear Power Plant Piping—Part 1: Quantification of Thickness Measurement Deviation

    Directory of Open Access Journals (Sweden)

    Hun Yun

    2016-06-01

    Full Text Available Pipe wall thinning by flow-accelerated corrosion and various types of erosion is a significant and costly damage phenomenon in secondary piping systems of nuclear power plants (NPPs. Most NPPs have management programs to ensure pipe integrity due to wall thinning that includes periodic measurements for pipe wall thicknesses using nondestructive evaluation techniques. Numerous measurements using ultrasonic tests (UTs; one of the nondestructive evaluation technologies have been performed during scheduled outages in NPPs. Using the thickness measurement data, wall thinning rates of each component are determined conservatively according to several evaluation methods developed by the United States Electric Power Research Institute. However, little is known about the conservativeness or reliability of the evaluation methods because of a lack of understanding of the measurement error. In this study, quantitative models for UT thickness measurement deviations of nuclear pipes and fittings were developed as the first step for establishing an optimized thinning evaluation procedure considering measurement error. In order to understand the characteristics of UT thickness measurement errors of nuclear pipes and fittings, round robin test results, which were obtained by previous researchers under laboratory conditions, were analyzed. Then, based on a large dataset of actual plant data from four NPPs, a quantitative model for UT thickness measurement deviation is proposed for plant conditions.

  8. Development of wall-thinning evaluation procedure for nuclear power plant piping-part 1: Quantification of thickness measurement deviation

    Energy Technology Data Exchange (ETDEWEB)

    Yun, Hun; Moon, Seung Jae [Dept. of Material Engineering, KEPCO Engineering and Construction, Sungnam (Korea, Republic of); Oh, Young Jin [Dept. of Mechanical Engineering, Hanyang University, Seoul (Korea, Republic of)

    2016-06-15

    Pipe wall thinning by flow-accelerated corrosion and various types of erosion is a significant and costly damage phenomenon in secondary piping systems of nuclear power plants (NPPs). Most NPPs have management programs to ensure pipe integrity due to wall thinning that includes periodic measurements for pipe wall thicknesses using nondestructive evaluation techniques. Numerous measurements using ultrasonic tests (UTs; one of the nondestructive evaluation technologies) have been performed during scheduled outages in NPPs. Using the thickness measurement data, wall thinning rates of each component are determined conservatively according to several evaluation methods developed by the United States Electric Power Research Institute. However, little is known about the conservativeness or reliability of the evaluation methods because of a lack of understanding of the measurement error. In this study, quantitative models for UT thickness measurement deviations of nuclear pipes and fittings were developed as the first step for establishing an optimized thinning evaluation procedure considering measurement error. In order to understand the characteristics of UT thickness measurement errors of nuclear pipes and fittings, round robin test results, which were obtained by previous researchers under laboratory conditions, were analyzed. Then, based on a large dataset of actual plant data from four NPPs, a quantitative model for UT thickness measurement deviation is proposed for plant conditions.

  9. Dose evaluation in special fluoroscopy procedures: Hysterosalpingography and Dacryocystography; Avaliacao de dose em procedimentos especiais de fluoroscopia: histerossalpingografia e dacriocistografia

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, Cintya Carolina Barbosa

    2006-04-15

    The hysterosalpingography (HSG) and dacryocystography (DCG) are among the special fluoroscopy procedures. The HSG is a radiodiagnostic technique used to detect uterine and tubal pathologies and it is fundamental for the investigation of infertility. The DCG is a form of lacrimal system imaging, being important to show the level of obstruction, the presence of dilatation of the lacrimal sac, as well as alterations in nearby structures. At this research, the study of skin entrance dose was evaluated for these two special fluoroscopy procedures, besides the analyses of staff doses whose performs the exams. The exams of 22 HSG patients and 8 DCG patients were evaluated using TL-100 dosimeters attached on patient' skin at anatomical landmarks evolved on each exam. In the case of HSG, the results showed that skin entrance doses varied from 0.5 mGy to 73.4 mGy, with an average value of 22.1 mGy. The estimated uterus dose was 5.5 mGy, and 6.6 mGy was the average dose estimated to the ovaries. The patient' skin entrance dose undergoing to DCG examinations varied from 2.1 mGy to 10.6 mGy, and the average eye's dose was 6.1 mGy. The results of staff dose showed that, on HSG, the average dose on doctor's right hand was 4.3 mGy per examination. This value had to the fact that the physician introduces the contrast manually while all contrast exposures. In relation of DCG, the staff's dose values were nearby background radiation, evidencing that, inside of permitted limits, there is no risk for the physicians at this procedure. (author)

  10. Tru-cut needle pleural biopsy and cytology as the initial procedure in the evaluation of pleural effusion.

    Science.gov (United States)

    Botana Rial, Maribel; Briones Gómez, Andrés; Ferrando Gabarda, José Ramón; Cifuentes Ruiz, José Fernando; Guarín Corredor, María Juliana; Manchego Frach, Nuria; Cases Viedma, Enrique

    2014-08-01

    The evaluation of pleural effusion (PE) includes various techniques, including pleural biopsy (PB). Our aim was to study the diagnostic yield of Tru-Cut needle PB (TCPB) and to define clinical/radiological situations in which TCPB might be indicated as an initial procedure. Retrospective study of TCPB in a hospital centre (2010-2012). Cases of pleural lesions without effusion were excluded. Clinical and radiological variables, diagnostic yield, TCPB complications and factors associated with the diagnostic yield of the combination of TCPB and thoracocentesis as initial procedure were analysed. One hundred and twenty-seven (127) TCPB were reviewed: 29.1% were cases of malignant PE and in 18.9% the cause of the PE could not be determined. The diagnostic yield of TCPB for tuberculosis was 76.5% (13/17) and 54% (20/37) for malignant PE. Complications occurred in 4.7% of the cases. In 72 patients with a final definitive diagnosis, TCPB was performed at the same time as the initial thoracocentesis. Diagnostic yield for the combination of TCPB/cytology as an initial technique was 43% (31/72) compared to 12.5% (9/72) for cytology only (p=0.01). The only predictive variable for the indication of TCBP as an initial technique was a PE volume>2/3 (P=.04). TCPB is safe and provides an acceptable diagnostic yield, particularly when combined with simultaneous cytology in the evaluation of PE of various aetiologies. Radiological criteria may help guide the selection of patients who could benefit from this technique as an initial procedure combined with thoracocentesis. Copyright © 2013 SEPAR. Published by Elsevier Espana. All rights reserved.

  11. Evaluation procedures in health: Perspective of nursing care in patient safety.

    Science.gov (United States)

    da Costa, Theo Duarte; Santos, Viviane Euzébia Pereira; Junior, Marcos Antônio Ferreira; Vitor, Alynne Fortes; de Oliveira Salvador, Pétala Tuani Candido; Alves, Kisna Yasmin Andrade

    2017-06-01

    The objective research is analyzing the nursing care in intensive care units from the perspective of patient safety based on health evaluation. This is an evaluation research, for the purpose of issuance of judgment or judgment on a given system, carried out in six intensive care units. Data collection occurred from April to July 2014, in locu, with a validated instrument containing 97 questions related to patient safety. These, 73 items targeted to analyze the element "process" in safety patient nursing care. The 73 items were grouped into three elements meaning of the patient safety: "Communication and Identification", "Health and Comfort" and "Drug and Nutritional Therapy". Data analyses were used from Kappa measurement, observations conducted by the evaluators and literature on the theme. The result of three elements significant showed the following: 23 items (31.5%) were considered adequate and 50 (68.4%), non-compliant with the required standards for reliable care. Of these, 29 (39.7%) were classified as partially adequate and 21 (28.7%) as inadequate, setting a worrying care in regards care of security with large probability precipitation of undesirable events. It is emphasized that the classification unsuitability of items prevailed. Patient safety is impaired due to unsafe actions in nursing care processes. Unsafe actions in care processes increase the risk to patient safety, as precipitation falls, errors in medication administration, communication difficulties and continuity of care. Thus, immediate interventions are imperative to implement a safety culture and to avoid negligence in relation to care. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. Flip-registration procedure of three-dimensional laser surface scanning images on quantitative evaluation of facial asymmetries.

    Science.gov (United States)

    Yu, Zheyuan; Mu, Xiongzheng; Feng, Shengzhi; Han, Jiayi; Chang, Tisheng

    2009-01-01

    The aim of the study was to find a proper method that may evaluate the severity of facial asymmetry quickly and accessibly in clinics. The three-dimensional image data of facial asymmetry patients were collected with three-dimensional laser surface scanning, and the desired therapy outcomes were simulated in computers by flip-registration procedure. The discrepancy between desired results and initial images was calculated automatically, and a colored hypsography was printed. A questionnaire was given to both the patient and the craniofacial surgeon to examine the symmetry, accessibility, achievability, and helpfulness of these outcomes. The three-dimensional image data offered by laser surface scanning were accurate and convenient. The desired results were reliable and acceptable to the patients. The colored hypsography was clear and accessible and achieved high appreciations from the surgeons. Three-dimensional laser surface scanning together with flip registration procedure can evaluate the severity of facial asymmetry quickly, quantitatively, and effectively with an achievable outcome. It is welcomed by the craniofacial surgeons and has a great potential in clinic application.

  13. Running-based Anaerobic Sprint Test As A Procedure To Evaluate Anaerobic Power

    OpenAIRE

    Andrade; V. L.; Zagatto; A. M.; Kalva-Filho; C. A.; Mendes; O. C.; Gobatto; C. A.; Campos; E. Z.; Papoti; M.

    2016-01-01

    The aim of this study was to evaluate the use of the running anaerobic sprint test (RAST) as a predictor of anaerobic capacity, compare it to the maximal accumulated oxygen deficit (MAOD) and to compare the RAST's parameters with the parameters of 30-s all-out tethered running on a treadmill. 39 (17.0+/-1.4 years) soccer players participated in this study. The participants underwent an incremental test, 10 submaximal efforts [50-95% of velocity correspondent to VO2MAX (vVO(2MAX))] and one sup...

  14. Preoperative evaluation, surgical procedure, follow up and results of 150 cochlear implantations.

    Science.gov (United States)

    Kyriafinis, G; Vital, V; Psifidis, A; Constantinidis, J; Nikolaou, A; Hitoglou-Antoniadou, M; Kouloulas, A

    2007-04-01

    The cochlear implantation is among the most important achievements of medicine and biotechnology in the last 20 years, because it allows individuals who had never heard or had lost their hearing to perceive sound and improve their quality of life. Selection criteria for candidates are strict and are evaluated in each individual by a scientific committee specially trained for implantations which includes Ear Nose and Throat (ENT) surgeon, audiologist, psychiatrist and speech therapist. In our department, the first cochlear implantation was performed in 1995. During the last ten years more than 250 individuals have been evaluated due to profound hearing loss and 170 of them were found to be suitable candidates for cochlear implantation. One hundred and fifty (150) have already been operated and most of them are children with congenital hearing loss. No major or permanent complications were recorded in any of our 150 patients. Activation and fitting/mapping of the cochlear implant is initiated three weeks post-operatively. Regular follow-up and mapping of the implant are held, more frequently in children, along with specialized speech therapy. Each new mapping is evaluated according to the record of the patient with regard to the acoustic perception of sounds and speech and the discrimination of individual elements of phonation based on a protocol that we have created for the needs of Greek language. Speech discrimination (AHEPA Hospital protocol), before the Implantation, at the activation of the cochlear implant and till 4 years of the follow-up showed that in our patients, we obtained better and faster results in post-speech acquisition adults with recent or chronic deafness and in children with congenital deafness operated before the 5th year of age, who underwent special preoperative speech therapy programme, fact which is in agreement with current literature. Patient satisfaction evaluated by "Sanders" psychometrics tests, was achieved in accordance to pre

  15. HBV DNA genome co-transfection procedure for the evaluation of relative fitness.

    Directory of Open Access Journals (Sweden)

    Ina Sevic

    Full Text Available Hepatitis B virus (HBV has a high mutation rate and exists as a mixture of genetically different but closely related variants. We present a HBV DNA co-transfection fitness assay and use it to evaluate the relative fitness of different HBV variants in two scenarios: seroconversion process and occupation of an ecological niche. In the seroconversion experiment, subgenotype D1 (sgtD1 deletion (1763-1770 had significantly lower fitness comparing with both sgtD1 wild type and sgtD1mut G1896A, while, in the case of occupation of ecological niche experiment, the results showed the same relative fitness between all of the genotype combinations, except F1b-F4. In this case sgtF1b clearly overgrow sgtF4, which is in accordance with the observation that F1b is the most prevalent in the new infections in Argentina. In summary, we present a method aimed to evaluate HBV viral fitness which improve the analysis of the relative frequency of viral variants during the HBV infection process.

  16. ENDF-102 DATA FORMATS AND PROCEDURES FOR THE EVALUATION NUCLEAR DATA FILE ENDF-6.

    Energy Technology Data Exchange (ETDEWEB)

    MCLANE,V.

    2001-05-15

    The Evaluated Nuclear Data File (ENDF) formats and libraries are decided by the Cross Section Evaluation Working Group (CSEWG), a cooperative effort of national laboratories, industry, and universities in the U.S. and Canada, and are maintained by the National Nuclear Data Center (NNDC). Earlier versions of the ENDF format provided representations for neutron cross sections and distributions, photon production from neutron reactions, a limited amount of charged-particle production from neutron reactions, photo-atomic interaction data, thermal neutron scattering data, and radionuclide production and decay data (including fission products). Version 6 (ENDF-6) allows higher incident energies, adds more complete descriptions of the distributions of emitted particles, and provides for incident charged particles and photonuclear data by partitioning the ENDF library into sub-libraries. Decay data, fission product yield data, thermal scattering data, and photo-atomic data have also been formally placed in sub-libraries. In addition, this rewrite represents an extensive update to the Version V manual.

  17. Categorical evaluation of the ocular irritancy of cosmetic and consumer products by human ocular instillation procedures.

    Science.gov (United States)

    Gao, Yang; Kanengiser, Bruce E

    2004-01-01

    The assessment of ocular irritation potential is an important part of safety testing for cosmetic and consumer products. The purpose of this investigation was to examine ocular irritancy levels elicited in humans by various categories of a specific class of cosmetic and consumer products that have a potential to enter the eye inadvertently during use. Test materials assessed belonged to one of seven categories, which included liquid makeup, shampoo, baby wash, mascara, eye makeup remover, powder eye shadow, and facial cleanser. These test materials were evaluated by human ocular instillation, followed by examinations, for which subjective perceptions of irritation were recorded, and component areas of ocular tissues were individually examined for inflammation and for the area and density of fluorescein staining patterns at 30 seconds and at 5, 15, 60, and 120 minutes post-instillation. Subjective and objective ocular irritation scores of 410 eyes were analyzed by product classification. Average score levels were determined for subjective responses, inflammation, and fluorescein staining patterns. This investigation determined that irritation levels of the evaluated test materials varied markedly with respect to product category, type of ocular irritation, and ocular tissue, demonstrating that these factors are important considerations for the prediction of the ocular irritancy of a test material.

  18. Custo do transplante hepático no Hospital de Clínicas da Universidade Federal do Paraná Cost of liver transplantation at the Clinical Hospital of the Federal University of Parana, Brazil

    Directory of Open Access Journals (Sweden)

    J.C.U. Coelho

    1997-03-01

    Full Text Available OBJETIVO. Determinar o custo do transplante hepático no Hospital de Clínicas da Universidade Federal do Paraná. MÉTODO. Os dados do prontuário de 24 pacientes submetidos a 25 transplantes hepáticos foram avaliados do dia da internação para o transplante hepático até a data da alta hospitalar ou óbito para determinar o número de dias de internação, o local de internação, a quantidade de material e medicamentos usados, os exames complementares e procedimentos realizados. Honorários médicos não foram incluídos no estudo. RESULTADOS. A idade dos pacientes variou de 6 a 56 anos, tendo seis deles menos que 14 anos de idade. Cinco pacientes foram a óbito durante a internação hospitalar. Retransplante foi realizado em somente um paciente. O custo médio da retirada do fígado do doador foi de US$ 2,783.19. O custo total do transplante hepático variou amplamente entre os pacientes, na dependência de ocorrência de complicações pós-operatórias, do número de dias de internação hospitalar e da quantidade de transfusão de hemoderivados. O custo total variou de US$ 6,359.84 a US$ 75,434.18, com média de US$ 21,505.53. O item mais caro do transplante hepático foi o custo com a hemoterapia, seguido do custo com medicamentos e diária hospitalar. CONCLUSÃO. O custo do transplante hepático varia muito entre os pacientes e pode ser realizado no Brasil a um custo inferior ao relatado nos Estados Unidos e na Europa.PURPOSE - To determine the cost of liver transplantation at the Clinical Hospital of the Federal University of Parana. METHODS - The data of 24 patients subjected to 25 liver transplantations were evaluated from the day of hospital admission until the day of discharge to determine the length of hospitalization, quantity of material and medications used, and exams and procedures performed. Professional fees were not included in the study. RESULTS - The age of the patients varied from 6 to 56 years. Six patients were

  19. Induction of apoptosis in HepG2 cells by solanine and Bcl-2 protein.

    Science.gov (United States)

    Ji, Y B; Gao, S Y; Ji, C F; Zou, X

    2008-01-17

    The nightshade (Solanum nigrum Linn.) has been widely used in Chinese traditional medicine as a remedy for the treatment of digestive system cancer. The anti-tumor activity of solanine, a steroid alkaloid isolated from the nightshade has been demonstrated. To observe the effect of anti-tumor and mechanism of solanine. The MTT assay was used to evaluate the IC(50) on the three digestive system tumor cell lines. The effect on the morphology was observed with a laser confocal microscopy; the rate of apoptosis and the cell cycle were measured using flow cytometry (FCM); the expression of Bcl-2 protein was measured by Western blot. The results show that the IC(50) for HepG(2), SGC-7901, and LS-174 were 14.47, >50, and >50 microg/ml, respectively; the morphology of cells in the negative control was normal; for the treated groups, typical signs for apoptosis were found. The rate of apoptosis in HepG(2) cells induced by solanine was found to be 6.0, 14.4, 17.3, 18.9, and 32.2%, respectively. Observation of the cell cycle showed that cells in the G(2)/M phases disappeared while the number of cells in the S phase increased significantly for treated groups. Western blot showed that solanine decreased the expression of Bcl-2 protein. Therefore, the target of solanine in inducing apoptosis in HepG(2) cells seems to be mediated by the inhibition in the expression of Bcl-2 protein.

  20. EVALUATION OF A BUFFERED SOLID PHASE DISPERSION PROCEDURE ADAPTED FOR PESTICIDE ANALYSES IN THE SOIL MATRIX

    Directory of Open Access Journals (Sweden)

    Ana María Domínguez

    2015-08-01

    Full Text Available An evaluation of the pesticides extracted from the soil matrix was conducted using a citrate-buffered solid phase dispersion sample preparation method (QuEChERS. The identification and quantitation of pesticide compounds was performed using gas chromatography-mass spectrometry. Because of the occurrence of the matrix effect in 87% of the analyzed pesticides, the quantification was performed using matrix-matched calibration. The method's quantification limits were between 0.01 and 0.5 mg kg-1. Repeatability and intermediate precision, expressed as a relative standard deviation percentage, were less than 20%. The recoveries in general ranged between 62% and 99%, with a relative standard deviation < 20%. All the responses were linear, with a correlation coefficient (r ≥0.99.

  1. Evaluating the performance of two neutron spectrum unfolding codes based on iterative procedures and artificial neural networks

    Science.gov (United States)

    Ortiz-Rodríguez, J. M.; Reyes Alfaro, A.; Reyes Haro, A.; Solís Sánches, L. O.; Miranda, R. Castañeda; Cervantes Viramontes, J. M.; Vega-Carrillo, H. R.

    2013-07-01

    In this work the performance of two neutron spectrum unfolding codes based on iterative procedures and artificial neural networks is evaluated. The first one code based on traditional iterative procedures and called Neutron spectrometry and dosimetry from the Universidad Autonoma de Zacatecas (NSDUAZ) use the SPUNIT iterative algorithm and was designed to unfold neutron spectrum and calculate 15 dosimetric quantities and 7 IAEA survey meters. The main feature of this code is the automated selection of the initial guess spectrum trough a compendium of neutron spectrum compiled by the IAEA. The second one code known as Neutron spectrometry and dosimetry with artificial neural networks (NDSann) is a code designed using neural nets technology. The artificial intelligence approach of neural net does not solve mathematical equations. By using the knowledge stored at synaptic weights on a neural net properly trained, the code is capable to unfold neutron spectrum and to simultaneously calculate 15 dosimetric quantities, needing as entrance data, only the rate counts measured with a Bonner spheres system. Similarities of both NSDUAZ and NSDann codes are: they follow the same easy and intuitive user's philosophy and were designed in a graphical interface under the LabVIEW programming environment. Both codes unfold the neutron spectrum expressed in 60 energy bins, calculate 15 dosimetric quantities and generate a full report in HTML format. Differences of these codes are: NSDUAZ code was designed using classical iterative approaches and needs an initial guess spectrum in order to initiate the iterative procedure. In NSDUAZ, a programming routine was designed to calculate 7 IAEA instrument survey meters using the fluence-dose conversion coefficients. NSDann code use artificial neural networks for solving the ill-conditioned equation system of neutron spectrometry problem through synaptic weights of a properly trained neural network. Contrary to iterative procedures, in neural

  2. Encefalopatía Hepática

    Directory of Open Access Journals (Sweden)

    Juan Carlos Restrepo Gutiérrez

    2008-12-01

    Full Text Available En pacientes con enfermedad hepática aguda o crónica la encefalopatía hepática es una complicación progresiva y reversible en su mayoría de veces, que puede reducir la sobrevida y la calidad de vida de quienes la presenten. Dentro de su patogénesis existen diferentes hipótesis entre las cuales la más aceptada ha sido la de la neurotoxicidad por amonio, que incluso podría explicar las otras hipótesis planteadas, como la del edema de los astrocitos, la toxicidad por manganeso, entre otras. La clasificación actual de la encefalopatía hepática se propuso en el congreso mundial de Gastroenterología en 1998 y se basa en la naturaleza de la disfunción hepática, duración y características de las manifestaciones neurológicas. Para su diagnostico se requiere de un buen reconocimiento clínico puesto que es principalmente de exclusión; sin embargo actualmente se cuenta con los criterios de West Haven para clasificar la severidad de su presentación. En este síndrome el tratamiento comprende diferentes elementos –como la dieta proteica, disacáridos no absorbibles y terapia antibiótica, que tienen como finalidad la resolución de las manifestaciones y mejorar la calidad de vida de los pacientes, aunque sin dejar de lado que tratar los factores precipitantes son la principal medida terapéutica para la encefalopatía hepática. El objetivo de este artículo es revisar los últimos conceptos de la encefalopatía hepática; con especial énfasis en su patogénesis, clasificación, diagnostico y tratamiento.

  3. Structural and enzymatic analyses of a glucosyltransferase Alr3699/HepE involved in Anabaena heterocyst envelop polysaccharide biosynthesis

    National Research Council Canada - National Science Library

    Wang, Xue-Ping; Jiang, Yong-Liang; Dai, Ya-Nan; Cheng, Wang; Chen, Yuxing; Zhou, Cong-Zhao

    2016-01-01

    .... The maturation of HEP in Anabaena sp. strain PCC 7120 is controlled by a gene cluster termed HEP island in addition to an operon alr3698-alr3699, which encodes two putative proteins termed Alr3698/HepD and Alr3699/HepE...

  4. A GIS-Based Procedure for Landslide Intensity Evaluation and Specific risk Analysis Supported by Persistent Scatterers Interferometry (PSI

    Directory of Open Access Journals (Sweden)

    Silvia Bianchini

    2017-10-01

    Full Text Available The evaluation of landslide specific risk, defined as the expected degree of loss due to landslides, requires the parameterization and the combination of a number of socio-economic and geological factors, which often needs the interaction of different skills and expertise (geologists, engineers, planners, administrators, etc.. The specific risk sub-components, i.e., hazard and vulnerability of elements at risk, can be determined with different levels of detail depending on the available auxiliary data and knowledge of the territory. These risk factors are subject to short-term variations and nowadays turn out to be easily mappable and evaluable through remotely sensed data and GIS (Geographic Information System tools. In this work, we propose a qualitative approach at municipal scale for producing a “specific risk” map, supported by recent satellite PSI (Persistent Scatterer Interferometry data derived from SENTINEL-1 C-band images in the spanning time 2014–2017, implemented in a GIS environment. In particular, PSI measurements are useful for the updating of a landslide inventory map of the area of interest and are exploited for the zonation map of the intensity of ground movements, needed for evaluating the vulnerability over the study area. Our procedure is presented throughout the application to the Volterra basin and the output map could be useful to support the local authorities with updated basic information required for environmental knowledge and planning at municipal level. Moreover, the proposed procedure is easily managed and repeatable in other case studies, as well as exploiting different SAR sensors in L- or X-band.

  5. Evaluation of a simulation procedure designed to recognize shape and contour of suspicious masses in mammography

    Science.gov (United States)

    Sousa, Maria A. Z.; Siqueira, Paula N.; Schiabel, Homero

    2015-03-01

    A large number of breast phantoms have been developed for conducting quality tests, characterization of imaging systems and computer aided diagnosis schemes, dosimetry and image perception. The realism of these phantoms is important for ensuring the accuracy of results and a greater range of applications. In this work, a developed phantom is considered proposing the use of PVC films for simulation of nodules inserted in the breast parenchyma designed for classification between malignant and benign signals according to the BI-RADS® standard. The investigation includes analysis of radiographic density, mass shape and its corresponding contour outlined by experienced radiologists. The material was cut based on lesions margins found in 44 clinical cases, which were divided between circumscribed and spiculated structures. Tests were performed to check the ability of the specialists in distinguishing the contour compared to actual cases while the shapes accuracy was determined quantitatively by evaluation metrics. Results showed the applicability of the chosen material creating image radiological patterns very similar to the actual ones.

  6. Synchronous evaluation of toxico- and pharmaco-dynamics of yttrium-90 by a novel autoradiographic procedure.

    Science.gov (United States)

    Shigematsu, Akiyo; Kawai, Naoshi; Kuroiwa, Hiroyuki; Higashi, Noriko; Debori, Yasuyuki; Yui, Joji; Hamai, Yuko; Hatori, Akiko; Awazu, Shoji; Takeuchi, Norihiro; Yamabayashi, Hisamichi; Sato, Yuichi; Yamamoto, Takeo

    2004-12-01

    A synchronous evaluation was performed, using a quick in vivo [2-(14)C]thymidine labeling method, of the toxico- and pharmaco-dynamics of a given dose of yttrium-90 (90Y) at a given time after injection to nude BALB/c mice loaded with 10(7) HuO9 cells. Quantitative data were 14C-microautoradiographs of the liver lobule, intestinal crypts, epiphysial growth plate, secondary ossification center containing pluripotent stem cells, perifollicular zone containing unipotent stem cells in the spleen, and plasmacytoma cells in the osteogenic sarcoma in each mouse following a 10-min labeling with 14C at 0.5, 6, and 24 h after i.v. injection of 90Y. Results show that the cell proliferation rate of the stem cells in respective tissues was markedly suppressed, dependent on time after dosing and the dose of 90Y; 3.7, 37, 370, 3700, and 37,000 kBq per mouse (25 g). In addition to the above, the sensitivity of the proliferation rate was dependent on amitosis or mitosis and the AUC value of 90Y-concentration at specific locations of the cells in the mouse body. The most sensitive cells were the plasmacytoma cells, followed by the pluripotent and unipotent stem cells, the intestinal crypts, epiphysial growth plate, and liver cells.

  7. In vitro evaluation of the effects of different treatment procedures on dentine tubules.

    Science.gov (United States)

    Gürsoy, Hare; Cakar, Gökser; Ipçi, Sebnem Dirikan; Kuru, Bahar; Yilmaz, Selçuk

    2012-12-01

    The objective of this preliminary study was to evaluate the occluding effect of topical gaseous ozone application and Er:YAG laser on human dentine tubules by scanning electron microscopic (SEM) analysis. Twenty-four dentine specimens were divided into three groups. Group I, including specimens treated only with citric acid, served as the control. Group II was treated with Er:YAG (30 Hz 60 mJ, 10 sec), and Group III received only topical gaseous ozone application (10 sec at level 2). Diameters and the number of open dentinal tubules per 100 μm(2) were counted from SEM photomigrophraphs at ×2000 magnification. In terms of diameters and numbers of open dentinal tubules per 100 μm(2), both treatment modalities presented significant occlusion. The mean values of the diameters and the number of open dentinal tubules per 100 μm(2) were observed in Group I, Group II, and Group III, in decreasing order. Significant differences were found among the groups in favor of the topical gaseous ozone applied group. The dentine tubules in both treatment groups were occluded, however more marked occlusion were seen in ozone treated group.

  8. Clinical evaluation of the biological width following surgical crown-lengthening procedure: A prospective study

    Directory of Open Access Journals (Sweden)

    Shobha K

    2010-01-01

    Full Text Available Background and Objectives: The purpose of this study was to evaluate the positional changes of the periodontal tissues, particularly the biological width, following surgical crown-lengthening in human subjects. Materials and Methods: A clinical trial study involving 15 patients was carried out for a period of 6 months. Sites were divided into 3 groups: treated (TT sites, adjacent (AD sites and nonadjacent (NAD sites. Free gingival margin [FGM], attachment level, pocket depth, bone level, biological width [BW] were recorded at baseline, 1, 3 and 6 months. Direct bone level after flap reflection was recorded before and after osseous resection at baseline only. Level of osseous crest was lowered based on BW, and supracrestal tooth structure needed using a combination of rotary and hand instruments. Statistical Analysis: Student t test and ANOVA were used. Results: Overall, apical displacement of FGM at TT, AD and NAD sites was statistically significant compared to baseline. The apical displacement of FGM at TT site was more when compared to that at AD and NAD sites at 3 and 6 months. The BW at the TT site was smaller at 1, 3 and 6 months compared to that at baseline. However, at all sites, BW was reestablished to the baseline value at the end of 6 months. Interpretation and Conclusion: The BW at TT sites was reestablished to its original vertical dimension by 6 months. In addition, a consistent 2-mm gain of coronal tooth structure was observed at the 1, 3 and 6-month examinations.

  9. Experimental evaluation of the efficacy of sanitation procedures in operating rooms.

    Science.gov (United States)

    Frabetti, Alessia; Vandini, Alberta; Balboni, Piergiorgio; Triolo, Fabio; Mazzacane, Sante

    2009-10-01

    There remains much debate on how to define an adequate sanitation protocol in hospital environments. The efficacy of a sanitation protocol in the operating room (OR) of a modern hospital was evaluated by measuring bacterial load on different types of finishing materials of all internal surfaces (ie, walls, floors, and furnishings). Samples were obtained before cleaning and over the subsequent 24 hours. A total of 2124 microbiological samples were collected using RODAC plates and sterile swabs. The data demonstrate a very significant postsanitation reduction of bacterial load on floors and furnishings; however, no significant data on walls were obtained, because of the low levels of initial contamination (1.50 to 5.98 cfu/100 cm2). The increase in postsanitation bacterial load over time was greater on smooth materials than on porous materials, on which a further reduction in contamination was seen. The study outcomes were confirmed by simulation experiments in which different materials were contaminated with a predetermined bacterial load and then subjected to the sanitation protocol. These simulation experiments were carried out both in vitro and in an eddy-flux testing room that simulated a full-scale OR similar (in terms of architectonic systems) to a real setting. Our data demonstrate that the spatial (vertical/horizontal) disposition of materials affects the initial contamination level, which is always much lower on vertical surfaces than on horizontal ones. Moreover, postsanitation bacterial load recovery is dependent on the physical properties of the surface.

  10. MDCT evaluation of pulmonary embolism in children and young adults following a lateral tunnel Fontan procedure: optimizing contrast-enhancement techniques

    Energy Technology Data Exchange (ETDEWEB)

    Prabhu, Sanjay P.; Mahmood, Soran; Sena, Laureen [Children' s Hospital Boston and Harvard Medical School, Department of Radiology, Boston, MA (United States); Lee, Edward Y. [Children' s Hospital Boston and Harvard Medical School, Department of Radiology, Boston, MA (United States); Children' s Hospital Boston and Harvard Medical School, Department of Medicine, Pulmonary Division, Boston, MA (United States)

    2009-09-15

    Pulmonary embolism (PE) is a life-threatening thromboembolic complication in patients who have undergone a Fontan procedure for augmenting pulmonary blood flow in the setting of single-ventricle physiology. In patients following a Fontan procedure, lack of proper contrast agent mixing in the right atrium and sluggish, low-velocity blood flow within the Fontan circulation often results in suboptimal contrast enhancement within the pulmonary artery for evaluating PE. Unfortunately, there is a paucity of information describing the optimal contrast-enhancement technique with multidetector CT (MDCT) for evaluating PE in children and young adults following a Fontan procedure. We illustrate the MDCT imaging findings of suboptimal contrast enhancement within the pulmonary artery, which can be mistaken for PE, in patients following a lateral Fontan procedure, and we discuss MDCT techniques to optimize contrast enhancement within the pulmonary artery in these patients for evaluating PE. The MDCT imaging findings in pediatric and young adult patients following a lateral Fontan procedure and with clinically suspected PE are illustrated. We describe intravenous contrast agent injection techniques that can be used to optimize the contrast enhancement in the pulmonary artery in patients following a lateral Fontan procedure. The use of a suboptimal contrast-enhancement technique led to initial misdiagnosis and incomplete evaluation of PE in the three patients following a lateral Fontan procedure. Imaging in two patients showed that optimal evaluation of thrombosis in the Fontan pathway and PE in the pulmonary arteries can be successfully achieved with simultaneous upper- and lower-limb injections of contrast agent. This series demonstrates that suboptimal contrast enhancement can result in misdiagnosis or incomplete evaluation of PE in children and young adults following a lateral Fontan procedure. Careful attention to optimizing contrast enhancement during MDCT examination for

  11. SU-E-J-69: Evaluation of the Lens Dose On the Cone Beam IGRT Procedures

    Energy Technology Data Exchange (ETDEWEB)

    Palomo-Llinares, R; Gimeno-Olmos, J; Carmona Meseguer, V; Lliso-Valverde, F; Candela-Juan, C; Perez-Calatayud, J [Hospital La Fe, Valencia, Valencia (Spain); Pujades, M [National Dosimetry Center, Valencia, Valencia (Spain); Ballester, F [University of Valencia, Burjassot (Spain)

    2014-06-01

    Purpose: With the establishment of the IGRT as a standard technique, the extra dose that is given to the patients should be taken into account. Furthermore, it has been a recent decrease of the dose threshold in the lens, reduced to 0.5 Gy (ICRP ref 4825-3093-1464 on 21st April, 2011).The purpose of this work was to evaluate the extra dose that the lens is receive due to the Cone-Beam (CBCT) location systems in Head-and-Neck treatments. Methods: The On-Board Imaging (OBI) v 1.5 of the two Varian accelerators, one Clinac iX and one True Beam, were used to obtain the dose that this OBI version give to the lens in the Head-and-Neck location treatments. All CBCT scans were acquired with the Standard Dose Head protocol (100 kVp, 80 mA, 8 ms and 200 degree of rotation).The measurements were taken with thermoluminescence (TLD) EXTRAD (Harshaw) dosimeters placed in an anthropomorphic phantom over the eye and under 3 mm of bolus material to mimic the lens position. The center of the head was placed at the isocenter. To reduce TLD energy dependence, they were calibrated at the used beam quality. Results: The average lens dose at the lens in the OBI v 1.5 systems of the Clinac iX and the True Beam is 0.071 and 0.076 cGy/CBCT, respectively. Conclusions: The extra absorbed doses that receive the eye lenses due to one CBCT acquisition with the studied protocol is far below the new ICRP recommended threshold for the lens. However, the addition effect of several CBCT acquisition during the whole treatment should be taken into account.

  12. Efficacy of panoramic radiography as a screening procedure in dental examination compared with clinical evaluation

    Energy Technology Data Exchange (ETDEWEB)

    An, Seo Young; An, Chang Hyeon; Choi, Karp Shik [Kyungpook National Univ. School of Dentistry, Daegu (Korea, Republic of)

    2007-06-15

    To evaluate the efficacy of panoramic radiography by comparing the results of clinical examination with radiographic findings. We studied 190 patients (20 men and 170 women; mean age, 40 years; range, 22 to 68 years) who visited the health promotion center of Korea Medical Science Institute and were examined both clinically and by panoramic radiography. We compared results from both examinations. Treatment options by clinical examination were described as 'no treatment indicated', 'treatment of dental caries', 'removal of calculus', 'treatment of periodontal disease'. 'prothodonic treatment' and 'extraction of the third molar'. Findings taken from the panoramic radiography were: dental caries, peroapical lesion, alveolar bone loss, calculus deposition, retained root, impaction of the third molar, disease of maxillary sinus, bony change of mandibular condyle, etc. The prevalence of panoramic findings were: 37.9% of dental caries, 17.4% of periapical lesions, 44.7% of alveolar bone losses, 62.6% of calculi deposition. 7.9% of retained roots, 26.8% of third molar impactions, 6.3% of disease of maxillary sinus, 2.1% of bony changes of mandibular condlye and 35.8% of miscellaneous lesions. Abnormal conditions revealed by panoramic radiography which had not been discovered on clinical examination were: 24.2% of the patients had dental caries, 17.4% had periapical lesions, 7.4% had calculi deposition, 5.3% had retained roots, 15.3% had third molar impactions. The opposite cases were: 5.2% had dental caries, 12.6% had calculi deposition, and 9.5% had third molar impactions. The use of panoramic radiography as a supplement to the clinical examination might be a valuable screening technique.

  13. Evaluation of a microbiological screening and acceptance procedure for cryopreserved skin allografts based on 14 day cultures.

    Science.gov (United States)

    Pirnay, Jean-Paul; Verween, Gunther; Pascual, Bruno; Verbeken, Gilbert; De Corte, Peter; Rose, Thomas; Jennes, Serge; Vanderkelen, Alain; Marichal, Miriam; Heuninckx, Walter; De Vos, Daniel

    2012-06-01

    Viable donor skin is still considered the gold standard for the temporary covering of burns. Since 1985, the Brussels military skin bank supplies cryopreserved viable cadaveric skin for therapeutic use. Unfortunately, viable skin can not be sterilised, which increases the risk of disease transmission. On the other hand, every effort should be made to ensure that the largest possible part of the donated skin is processed into high-performance grafts. Cryopreserved skin allografts that fail bacterial or fungal screening are reworked into 'sterile' non-viable glycerolised skin allografts. The transposition of the European Human Cell and Tissue Directives into Belgian Law has prompted us to install a pragmatic microbiological screening and acceptance procedure, which is based on 14 day enrichment broth cultures of finished product samples and treats the complex issues of 'acceptable bioburden' and 'absence of objectionable organisms'. In this paper we evaluate this procedure applied on 148 skin donations. An incubation time of 14 days allowed for the detection of an additional 16.9% (25/148) of contaminated skin compared to our classic 3 day incubation protocol and consequently increased the share of non-viable glycerolised skin with 8.4%. Importantly, 24% of these slow-growing microorganisms were considered to be potentially pathogenic. In addition, we raise the issue of 'representative sampling' of heterogeneously contaminated skin. In summary, we feel that our present microbiological testing and acceptance procedure assures adequate patient safety and skin availability. The question remains, however, whether the supposed increased safety of our skin grafts outweighs the reduced overall clinical performance and the increase in work load and costs.

  14. Evaluation of the results and complications of the Latarjet procedure for recurrent anterior dislocation of the shoulder

    Directory of Open Access Journals (Sweden)

    Luciana Andrade da Silva

    2015-12-01

    Full Text Available ABSTRACT OBJECTIVE: Evaluate the results and complications of Latarjet procedure in patients with anterior recurrent dislocation of the shoulder. METHODS: Fifty-one patients (52 shoulders with anterior recurrent dislocation, surgically treated by Latarjet procedure, were analyzed retrospectively. The average follow-up time was 22 months, range 12-66 months; The age range was 15-59 years with a mean of 31; regarding sex, 42 (82.4% patients were male and nine (17.6% were female. The dominant side was affected in 29 (55.8% shoulders. Regarding the etiology, 48 (92.3% reported trauma and four (7.6% had the first episode after a convulsion. RESULTS: The average elevation, lateral rotation and medial rotation of the operated shoulder were, respectively, 146° (60-80°, 59° (0-85° and T8 (T5 gluteus, with statistical significance for decreased range of motion in all planes, compared with the other side. The scores of Rowe and UCLA were 90.6 and 31.4, respectively, in the postoperative period. Eleven shoulders (21.2% had poor results: signs of instability (13.4%, non-union (11.5% and early loosening of the synthesis material (1.9%. There was a correlation between poor results and convulsive patients ( p = 0.026. CONCLUSION: We conclude that the Latarjet procedure for correction of anterior recurrent dislocation leads to good and excellent results in 82.7% of cases. Complications are related to errors in technique.

  15. Short-term in vivo evaluation of zinc-containing calcium phosphate using a normalized procedure

    Energy Technology Data Exchange (ETDEWEB)

    Calasans-Maia, Monica, E-mail: monicacalasansmaia@gmail.com [Dental Clinical Research Center, Dentistry School, Fluminense Federal University, Niteroi, Rio de Janeiro (Brazil); Calasans-Maia, José, E-mail: josecalasans@gmail.com [Dental Clinical Research Center, Dentistry School, Fluminense Federal University, Niteroi, Rio de Janeiro (Brazil); Santos, Silvia, E-mail: silviaquimica@gmail.com [LABIOMAT, Brazilian Center for Physics Research, CBPF, Rio de Janeiro (Brazil); Mavropoulos, Elena, E-mail: elena@cbpf.br [LABIOMAT, Brazilian Center for Physics Research, CBPF, Rio de Janeiro (Brazil); Farina, Marcos, E-mail: mfarina@anato.ufrj.br [Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro (Brazil); Lima, Inayá, E-mail: inayacorrea@gmail.com [Nuclear Instrumentation Laboratory, Nuclear Engineering Program, COPPE, Federal University of Rio de Janeiro, Rio de Janeiro (Brazil); Lopes, Ricardo Tadeu [Nuclear Instrumentation Laboratory, Nuclear Engineering Program, COPPE, Federal University of Rio de Janeiro, Rio de Janeiro (Brazil); Rossi, Alexandre, E-mail: rossi@cbpf.br [LABIOMAT, Brazilian Center for Physics Research, CBPF, Rio de Janeiro (Brazil); Granjeiro, José Mauro, E-mail: jmgranjeiro@gmail.com [Dental Clinical Research Center, Dentistry School, Fluminense Federal University, Niteroi, Rio de Janeiro (Brazil); Bioengineering Division, National Institute of Metrology, Quality and Technology, Duque de Caxias, Rio de Janeiro (Brazil)

    2014-08-01

    The effect of zinc-substituted calcium phosphate (CaP) on bone osteogenesis was evaluated using an in vivo normalized ISO 10993-6 protocol. Zinc-containing hydroxyapatite (ZnHA) powder with 0.3% by wt zinc (experimental group) and stoichiometric hydroxyapatite (control group) were shaped into cylindrical implants (2 × 6 mm) and were sintered at 1000 °C. Thermal treatment transformed the ZnHA cylinder into a biphasic implant that was composed of Zn-substituted HA and Zn-substituted β-tricalcium phosphate (ZnHA/βZnTCP); the hydroxyapatite cylinder was a highly crystalline and poorly soluble HA implant. In vivo tests were performed in New Zealand White rabbits by implanting two cylinders of ZnHA/βZnTCP in the left tibia and two cylinders of HA in the right tibia for 7, 14 and 28 days. Incorporation of 0.3% by wt zinc into CaP increased the rate of Zn release to the biological medium. Microfluorescence analyses (μXRF-SR) using synchrotron radiation suggested that some of the Zn released from the biomaterial was incorporated into new bone near the implanted region. In contrast with previous studies, histomorphometric analysis did not show significant differences between the newly formed bone around ZnHA/βZnTCP and HA due to the dissolution profile of Zn-doped CaP. Despite the great potential of Zn-containing CaP matrices for future use in bone regeneration, additional in vivo studies must be conducted to explain the mobility of zinc at the CaP surface and its interactions with a biological medium. - Highlights: • We produced a hydroxyapatite containing a low concentration (0.3 wt.%) of zinc. • The biomaterial underwent characterization before and after in vivo implant. • In vivo tests were performed according to ISO 10993-6. • Zinc-containing calcium phosphate promotes osteoconduction and bone regeneration. • Zinc-containing calcium phosphate may be useful for clinical applications.

  16. An Object-oriented minimization package for HEP

    Energy Technology Data Exchange (ETDEWEB)

    Mark S Fischler and David Sachs

    2003-07-02

    A portion of the HEP community has perceived the need for a minimization package written in C++ and taking advantage of the Object-Oriented nature of that language. To be acceptable for HEP, such a package must at least encompass all the capabilities of Minuit. Aside from the slight plus of not relying on outside Fortran compilation, the advantages that a C++ package based on O-O design would confer over the multitude of available C++ Minuit-wrappers include: Easier extensibility to different algorithms and forms of constraints; and usage modes which would not be available in the global-common-based Minuit design. An example of the latter is a job pursuing two ongoing minimization problems simultaneously. We discuss the design and implementation of such a package, which extends Minuit only in minor ways but which greatly diminishes the programming effort (if not the algorithm thought) needed to make more significant extensions.

  17. Towards Provenance and Traceability in CRISTAL for HEP

    Science.gov (United States)

    Shamdasani, Jetendr; Branson, Andrew; McClatchey, Richard

    2014-06-01

    This paper discusses the CRISTAL object lifecycle management system and its use in provenance data management and the traceability of system events. This software was initially used to capture the construction and calibration of the CMS ECAL detector at CERN for later use by physicists in their data analysis. Some further uses of CRISTAL in different projects (CMS, neuGRID and N4U) are presented as examples of its flexible data model. From these examples, applications are drawn for the High Energy Physics domain and some initial ideas for its use in data preservation HEP are outlined in detail in this paper. Currently investigations are underway to gauge the feasibility of using the N4U Analysis Service or a derivative of it to address the requirements of data and analysis logging and provenance capture within the HEP long term data analysis environment.

  18. Towards Provenance and Traceability in CRISTAL for HEP

    CERN Document Server

    Shamdasani, Jetendr; McClatchey, Richard

    2014-01-01

    This paper discusses the CRISTAL object lifecycle management system and its use in provenance data management and the traceability of system events. This software was initially used to capture the construction and calibration of the CMS ECAL detector at CERN for later use by physicists in their data analysis. Some further uses of CRISTAL in different projects (CMS, neuGRID and N4U) are presented as examples of its flexible data model. From these examples, applications are drawn for the High Energy Physics domain and some initial ideas for its use in data preservation HEP are outlined in detail in this paper. Currently investigations are underway to gauge the feasibility of using the N4U Analysis Service or a derivative of it to address the requirements of data and analysis logging and provenance capture within the HEP long term data analysis environment.

  19. Onfalopileflebitis y absceso hepático neonatal

    Directory of Open Access Journals (Sweden)

    Carlos Baeza-Herrera

    2015-05-01

    Full Text Available La onfalopileflebitis y el absceso hepático en neonatos son raros y se asocian, en la mayoría de las ocasiones, con infecciones por Staphylococcus epidermidis y como complicación de la cateterización venosa umbilical. La manifestación única es una infección sistémica e implica dificultades diagnósticas. Presentamos los hallazgos clínicos y quirúrgicos de un recién nacido masculino de 12 días con onfalopileflebitis y absceso hepático asociados con cateterización de la vena umbilical.

  20. docker & HEP: Containerization of applications for development, distribution and preservation

    Science.gov (United States)

    Binet, S.; Couturier, B.

    2015-12-01

    HEP software stacks are not shallow. Indeed, HEP experiments’ software is usually many applications in one (reconstruction, simulation, analysis,...) and thus require many libraries - developed in-house or by third parties - to be properly compiled and installed. Moreover, because of resource constraints, experiments’ software is usually installed, tested, validated and deployed on a very narrow set of platforms, architectures, toolchains and operating systems. As a consequence, bootstrapping a software environment on a developer machine or deploying the software on production or user machines is usually perceived as tedious and iterative work, especially when one wants the native performances of bare metal. Docker containers provide an interesting avenue for packaging applications and development environment, relying on the Linux kernel capabilities for process isolation, adding git-like capabilities to the filesystem layer and providing (close to) native CPU, memory and I/O performances.

  1. docker & HEP : containerization of applications for development, distribution and preservation

    CERN Document Server

    Binet, Sebastien

    2015-01-01

    HEP software stacks are not shallow. Indeed, HEP experiments' software are usually many applications in one (reconstruction, simulation, analysis, ...) and thus require many libraries - developed in-house or by third parties - to be properly compiled and installed. Moreover, because of resource constraints, experiments' software is usually installed, tested, validated and deployed on a very narrow set of platforms, architectures, toolchains and operating systems. As a consequence, bootstrapping a software environment on a developer machine or deploying the software on production or user machines is usually perceived as tedious and iterative work, especially when one wants the native performances of bare metal. `Docker` containers provide an interesting avenue for packaging applications and development environment, relying on the Linux kernel capabilities for process isolation, adding "git"-like capabilities to the filesystem layer and providing (close to) native CPU, memory and I/O performances. This paper wi...

  2. Using Procedure Based on Item Response Theory to Evaluate Classification Consistency Indices in the Practice of Large-Scale Assessment

    Directory of Open Access Journals (Sweden)

    Shanshan Zhang

    2017-09-01

    Full Text Available In spite of the growing interest in the methods of evaluating the classification consistency (CC indices, only few researches are available in the field of applying these methods in the practice of large-scale educational assessment. In addition, only few studies considered the influence of practical factors, for example, the examinee ability distribution, the cut score location and the score scale, on the performance of CC indices. Using the newly developed Lee's procedure based on the item response theory (IRT, the main purpose of this study is to investigate the performance of CC indices when practical factors are taken into consideration. A simulation study and an empirical study were conducted under comprehensive conditions. Results suggested that with negatively skewed distribution, the CC indices were larger than with other distributions. Interactions occurred among ability distribution, cut score location, and score scale. Consequently, Lee's IRT procedure is reliable to be used in the field of large-scale educational assessment, and when reporting the indices, it should be treated with caution as testing conditions may vary a lot.

  3. A histologic evaluation of various stages of palatal healing following subepithelial connective tissue grafting procedures: a comparison of eight cases.

    Science.gov (United States)

    Soileau, Kristi M; Brannon, Robert B

    2006-07-01

    It is often necessary to procure tissue from the same area of the palate in cases where connective autogenous grafting procedures are warranted due to limitations caused by anatomical features. The purpose of this study was to determine whether the length of time between a first procurement and a second would have any bearing on the quality of tissue available for recipient sites. Eight patients requiring more than one grafting procedure underwent surgery at baseline and again at various intervals ranging from 6 weeks to 11 months. Specimens were taken from the palate and evaluated microscopically, and photographs were taken for purposes of visual comparison. The 6.9- to 7.7-week specimens exhibited complete reepithelialization. The lamina propriae were composed of a cellular proliferation of fibroblasts with loosely arranged collagen deposition and an occasional thin vascular channel. However, remodeling of the wound appeared complete in the specimens removed at the 9-week interval and beyond. The lamina propria was, in general, composed of thick, dense, interlacing bundles of collagen. Small-caliber blood vessels were interspersed throughout the fibrous element. Reharvesting of tissue performed earlier than at 9 weeks may result in poorer autogenous graft quality due to indications that remodeling of the connective tissue is still progressing and not as mature as specimens noted at weeks 9 to 47.

  4. Evaluation of three different validation procedures regarding the accuracy of template-guided implant placement: an in vitro study.

    Science.gov (United States)

    Vasak, Christoph; Strbac, Georg D; Huber, Christian D; Lettner, Stefan; Gahleitner, André; Zechner, Werner

    2015-02-01

    The study aims to evaluate the accuracy of the NobelGuide™ (Medicim/Nobel Biocare, Göteborg, Sweden) concept maximally reducing the influence of clinical and surgical parameters. Moreover, the study was to compare and validate two validation procedures versus a reference method. Overall, 60 implants were placed in 10 artificial edentulous mandibles according to the NobelGuide™ protocol. For merging the pre- and postoperative DICOM data sets, three different fusion methods (Triple Scan Technique, NobelGuide™ Validation software, and AMIRA® software [VSG - Visualization Sciences Group, Burlington, MA, USA] as reference) were applied. Discrepancies between the virtual and the actual implant positions were measured. The mean deviations measured with AMIRA® were 0.49 mm (implant shoulder), 0.69 mm (implant apex), and 1.98°mm (implant axis). The Triple Scan Technique as well as the NobelGuide™ Validation software revealed similar deviations compared with the reference method. A significant correlation between angular and apical deviations was seen (r = 0.53; p < .001). A greater implant diameter was associated with greater deviations (p = .03). The Triple Scan Technique as a system-independent validation procedure as well as the NobelGuide™ Validation software are in accordance with the AMIRA® software. The NobelGuide™ system showed similar or less spatial and angular deviations compared with others. © 2013 Wiley Periodicals, Inc.

  5. Metabolomic effects in HepG2 cells exposed to four TiO2 amd two CeO2 naomaterials

    Science.gov (United States)

    Abstract It is difficult to evaluate nanomaterials potential toxicity and to make science-based societal choices. To better assess potential hepatotoxicity issues, human liver HepG2 cells were exposed to four Ti02 and two Ce02 nanomaterials at 30 ug m1-1 for t...

  6. The effect of chitooligosaccharides on oleic acid-induced lipid accumulation in HepG2 cells

    Directory of Open Access Journals (Sweden)

    Peiqiu Cao

    2016-05-01

    Full Text Available This experiment aimed to evaluate the capacities of two types of chitooligosaccharides (COS with different molecular weights for the ability to eliminate lipid accumulation in hepatocytes. We have established a lipid accumulation model in HepG2 cells for these studies in vitro, which was established by induction with oleic acid. The capacity of COS to eliminate lipid accumulation was evaluated using three metrics: the thiazolyl blue dye absorbance (MTT value, the morphology of intracellular lipid droplets and the triglyceride level (TG. Two types of COS with different molecular weights (1000 Da and 3000 Da can significantly reduce intracellular lipid accumulation and decrease TG content in HepG2 cells, in a dose-dependent fashion. We found that low molecular weight COS is more efficacious than high molecular weight COS. Two types of COS can eliminate lipid accumulation induced by oleic acid in HepG2 cells, leading to an obvious hypolipidemic effect in vitro. These results suggest that COS may be effective preventive agents in fatty liver disease.

  7. Hematoma hepático subcapsular en el puerperio

    OpenAIRE

    Carazo H,Belén; Romero C,Manuel Ángel; Puebla M,Cristina; Sanz M,Amelia; Rojas P,Beatriz

    2013-01-01

    El hematoma hepático subcapsular es una complicación infrecuente y grave durante la gestación o el período puerperal. Esta patología generalmente se relaciona con preeclampsia o síndrome de HELLP. Su diagnóstico debe confirmarse por tomografía axial computarizada. La precocidad del diagnóstico y tratamiento es importante para evitar la ruptura del hematoma.

  8. Ressecões hepáticas por videolaparoscopia Current status of laparoscopic liver resections

    Directory of Open Access Journals (Sweden)

    Paulo Herman

    2009-12-01

    Full Text Available INTRODUÇÃO: As ressecções hepáticas representam umas das últimas fronteiras vencidas pela cirurgia videolaparoscópica. Apesar da complexidade do procedimento, da demanda de grande incorporação de tecnologia e necessidade de experiência em cirurgia hepática e laparoscópica, a indicação do método tem crescido de forma expressiva nos últimos anos. OBJETIVO: Realizar análise crítica do método, baseada nos trabalhos existentes na literatura, ressaltando o estado atual de suas indicações, exequibilidade, segurança, resultados e aspectos técnicos primordiais. MÉTODO: Foram identificados e analisados os trabalhos pertinentes nas bases de dados LILACS e PUBMED até dezembro de 2009, utilizando-se os descritores "liver resection", "laparoscopic" e "liver surgery". Não foram encontrados trabalhos prospectivos e randomizados sobre o tema, sendo os dados disponíveis provenientes de série de casos, estudos caso-controle e alguns estudos multicêntricos e metanálises. CONCLUSÃO: A hepatectomia por videolaparoscopia é hoje operação segura e factível, mesmo para as ressecções hepáticas maiores, com baixo índice de morbimortalidade. O método pode ser utilizado para lesões malignas sem prejuízo dos princípios oncológicos e com vantagens nos pacientes com cirrose ou disfunção hepática. A melhor indicação recai sobre as lesões benignas, em especial o adenoma hepatocelular. Em mãos experientes e casos selecionados, como as lesões benignas localizadas nos segmentos anterolaterais hepáticos, principalmente no segmento lateral esquerdo, a ressecção videolaparoscópica pode ser considerada hoje como tratamento padrão.INTRODUCTION: Hepatic resection is the last frontier to be surpassed by laparoscopic surgery. Although a highly complex procedure, the need of advanced technology and experience in both laparoscopic and hepatic surgery, the indications and number of cases done worldwide had a major growth in the last few

  9. Alkaline phosphatase retained in HepG2 hepatocarcinoma cells vs. alkaline phosphatase released to culture medium: difference of aberrant glycosylation.

    Science.gov (United States)

    Nowrouzi, Azin; Yazdanparast, Razieh

    2005-05-06

    Liver tissue is the source of 90% of serum alkaline phosphatase (AP). The serum levels and structures of tumor marker proteins change under many disease conditions as well as cancer. The study was aimed at determining the type of alkaline phosphatase (AP) present in HepG2 hepatocellular carcinoma cell line. Alkaline phosphatase rich extracts of healthy human liver, HepG2 hepatocarcinoma cells, as well as the condition medium of HepG2 cells were prepared by extraction with 40% n-butanol and 30-50% acetone precipitation, and subjected to various chromatographic procedures. Lectin affinity chromatography of the samples with concanavalin A-Sepharose 4B showed considerable differences in the elution patterns. Non-denaturing polyacrylamide gel electrophoresis of the culture medium yielded a relatively slow migrating band of activity that coincided with none of the three bands of activity produced by the normal liver extract, nor with the bands of the cell pellet extract. Inhibition patterns were established by measuring the enzyme activities in the presence of varying concentrations of L-phenylalanine, L-leucine, L-homoarginine, and levamisole. The APs from the cell line were neuraminidase sensitive. According to the results the main AP produced and released to the medium by HepG2 cell line is an aberrantly glycosylated tissue non-specific AP. In addition, the differences between the cell-pellet AP and the culture medium AP seemed to stem from different sugar moieties in their structures.

  10. A novel AMPK activator, WS070117, improves lipid metabolism discords in hamsters and HepG2 cells

    Directory of Open Access Journals (Sweden)

    Hao Linghua

    2011-04-01

    Full Text Available Abstract Background WS070117 is a novel small molecule compound that significantly improves lipid metabolism disorders in high-fat-diet (HFD induced hyperlipidemia in hamsters. Methods and Results We evaluated liver/body weight ratio, liver histology, serum and hepatic lipid content in HFD-fed hamsters treated with WS070117 for 8 weeks. Comparing with HFD fed hamsters, WS070117 (2 mg/kg per day and above reduced serum triglyceride (TAG, total cholesterol (TC, low density lipoprotein cholesterol (LDL-C and hepatic cholesterol and triglyceride contents. Oil Red O staining of liver tissue also showed that WS070117 improved lipid accumulation. We then carried out an experiment in the oleic acid (OLA-induced steatosis model in HepG2 cell to investigate the lipid-lowering effect of WS070117. Oleic acid (0.25 mM markedly induced lipid accumulation in HepG2 cells, but WS070117 (10 μM inhibited cellular lipid accumulation. In OLA-treated HepG2 cells, WS070117 (above 1 μM treatment reduced lipid contents which synthesized from [1-14C] labeled acetic acid. Because WS070117 is an analog of adenosine, we evaluated the effect of WS070117 on AMP-activated protein kinase (AMPK signaling. The results showed that the activation of AMPK in OLA-induced steatosis in HepG2 cells was up-regulated by treatment with 0.1, 1 and 10 μM WS070117. The hepatic cellular AMPK phosphorylation is also up regulated by WS070117 (6 and 18 mg/kg treatment in HFD fed hamsters. Conclusion These new findings identify WS070117 as a novel molecule that regulates lipid metabolism in the hyperlipidemia hamster model. In vitro and in vivo studies suggested that WS070117 may regulate lipid metabolism through stimulating the activation of AMPK and its downstream pathways.

  11. Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2 cell line

    Directory of Open Access Journals (Sweden)

    Nonpunya Apiyada

    2011-10-01

    Full Text Available Abstract Background Six plants from Thailand were evaluated for their cytotoxicity and apoptosis induction in human hepatocarcinoma (HepG2 as compared to normal African green monkey kidney epithelial cell lines. Methods Ethanol-water crude extracts of the six plants were tested with neutral red assay for their cytotoxicity after 24 hours of exposure to the cells. Apoptotic induction was tested in the HepG2 cells with diamidino-2-phenylindole staining. DNA fragmentation, indicative of apoptosis, was analyzed with agarose gel electrophoresis. Alkylation, indicative of DNA damage, was also evaluated in vitro by 4-(4'-nitrobenzyl pyridine assay. Results The extract of Pinus kesiya showed the highest selectivity (selectivity index = 9.6 and potent cytotoxicity in the HepG2 cell line, with an IC50 value of 52.0 ± 5.8 μg/ml (mean ± standard deviation. Extract of Catimbium speciosum exerted cytotoxicity with an IC50 value of 55.7 ± 8.1 μg/ml. Crude extracts from Glochidion daltonii, Cladogynos orientalis, Acorus tatarinowii and Amomum villosum exhibited cytotoxicity with IC50 values ranging 100-500 μg/ml. All crude extracts showed different alkylating abilities in vitro. Extracts of P. kesiya, C. speciosum and C. orientalis caused nuclei morphological changes and DNA laddering. Conclusion The extracts of C. speciosum, C. orientalis and P. kesiya induced apoptosis. Among the three plants, P. kesiya possessed the most robust anticancer activity, with specific selectivity against HepG2 cells.

  12. Cytotoxicity and genotoxicity of stilbene derivatives in CHO-K1 and HepG2 cell lines

    Directory of Open Access Journals (Sweden)

    Cassia Suemi Mizuno

    2017-07-01

    Full Text Available Abstract The cytotoxicity and genotoxicity of the stilbenes (E-methyl-4-(3-5-dimethoxystyrylbenzoate (ester, (E-4-(3-5-dimethoxystyrylaniline (amino, (Z-1,3-dimethoxy-5-(4-methoxystyrylbenzene (cis-TMS and (E-1,3-dimethoxy-5-(4-methoxystyrylbenzene (trans-TMS were investigated in this work. Structural modifications of resveratrol, a naturally occurring stilbene, have been previously performed, including the replacement of hydroxyl by different functional groups. Such modifications resulted in significant improvement of target-specific effects on cell death and antiproliferative responses. The parameters were evaluated using XTT assay, clonogenic survival assay and the cytokinesis-block micronucleus assay in CHO-K1 and HepG2 cell lines. The results showed that cis-TMS is approximately 250-fold more cytotoxic than the amino and ester, and 128-fold more cytotoxic than trans-TMS. When genotoxicity was evaluated, only the trans-TMS did not significantly increase the frequency of micronucleus (MN. While the cis-TMS induced a mean of 5.2 and 5.9 MN/100 cells at 0.5 μM in CHO-K1 and HepG2, respectively, the amino and ester induced 3.1 and 3.6 MN/100 cells at 10 μM in CHO-K1, respectively, and 3.5 and 3.8 in HepG2. Trans-TMS is genotoxic only in HepG2 cells. Based on these results, the cis-TMS was the most cytotoxic and genotoxic compound in both cell lines.

  13. INSPIRE: a new scientific information system for HEP

    CERN Multimedia

    Ivanov, R

    2009-01-01

    The status of high-energy physics (HEP) information systems has been jointly analyzed by the libraries of CERN, DESY, Fermilab and SLAC. As a result, the four laboratories have started the INSPIRE project – a new platform built by moving the successful SPIRES features and content, curated at DESY, Fermilab and SLAC, into the open-source CDS Invenio digital library software that was developed at CERN. INSPIRE will integrate present acquisition workflows and databases to host the entire body of the HEP literature (about one million records), aiming to become the reference HEP scientific information platform worldwide. It will provide users with fast access to full-text journal articles and preprints, but also material such as conference slides and multimedia. INSPIRE will empower scientists with new tools to discover and access the results most relevant to their research, enable novel text- and data-mining applications, and deploy new metrics to assess the impact of articles and authors. In addition, it will ...

  14. INSPIRE: a new scientific information system for HEP

    CERN Document Server

    Ivanov, R; CERN. Geneva. IT Department

    2010-01-01

    The status of high-energy physics (HEP) information systems has been jointly analyzed by the libraries of CERN, DESY, Fermilab and SLAC. As a result, the four laboratories have started the INSPIRE project – a new platform built by moving the successful SPIRES features and content, curated at DESY, Fermilab and SLAC, into the open-source CDS Invenio digital library software that was developed at CERN. INSPIRE will integrate current acquisition workflows and databases to host the entire body of the HEP literature (about one million records), aiming to become the reference HEP scientific information platform worldwide. It will provide users with fast access to full text journal articles and preprints, but also material such as conference slides and multimedia. INSPIRE will empower scientists with new tools to discover and access the results most relevant to their research, enable novel text- and data-mining applications, and deploy new metrics to assess the impact of articles and authors. In addition, it will ...

  15. HEP-FCE Working Group on Libraries and Tools

    Energy Technology Data Exchange (ETDEWEB)

    Borgland, Anders [SLAC National Accelerator Lab., Menlo Park, CA (United States); Elmer, Peter [Princeton Plasma Physics Lab. (PPPL), Princeton, NJ (United States); Kirby, Michael [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Patton, Simon [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Potekhin, Maxim [Brookhaven National Lab. (BNL), Upton, NY (United States); Viren, Brett [Brookhaven National Lab. (BNL), Upton, NY (United States); Yanny, Brian [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)

    2014-12-19

    The High-Energy Physics Forum for Computational Excellence (HEP-FCE) was formed by the Department of Energy as a follow-up to a recent report from the Topical Panel on Computing[1] and the associated P5 recommendation[2]. It is a pilot project distributed across the DOE Labs. During this initial incubation period the Forum is to develop a plan for a robust, long-term organization structure and a functioning web presence for forum activities and outreach, and a study of hardware and software needs across the HEP program. In the following sections we give this working group’s “vision” for aspects and qualities we wish to see in a future HEP-FCE. We then give a prioritized list of technical activities with suggested scoping and deliverables that can be expected to provide cross-experiment benefits. The remaining bulk of the report gives a technical survey of some specific “areas of opportunity” for cross-experiment benefit in the realm of software libs/tools. This survey serves as support for the vision and prioritized list. For each area we describe the ways that cross-experiment benefit is achieved today, as well as describe known failings or pitfalls where such benefit has failed to be achieved and which should be avoided in the future. For both cases, we try to give concrete examples. Each area then ends with an examination of what opportunities exist for improvements in that particular area.

  16. HEP Computing Tools, Grid and Supercomputers for Genome Sequencing Studies

    Science.gov (United States)

    De, K.; Klimentov, A.; Maeno, T.; Mashinistov, R.; Novikov, A.; Poyda, A.; Tertychnyy, I.; Wenaus, T.

    2017-10-01

    PanDA - Production and Distributed Analysis Workload Management System has been developed to address ATLAS experiment at LHC data processing and analysis challenges. Recently PanDA has been extended to run HEP scientific applications on Leadership Class Facilities and supercomputers. The success of the projects to use PanDA beyond HEP and Grid has drawn attention from other compute intensive sciences such as bioinformatics. Recent advances of Next Generation Genome Sequencing (NGS) technology led to increasing streams of sequencing data that need to be processed, analysed and made available for bioinformaticians worldwide. Analysis of genomes sequencing data using popular software pipeline PALEOMIX can take a month even running it on the powerful computer resource. In this paper we will describe the adaptation the PALEOMIX pipeline to run it on a distributed computing environment powered by PanDA. To run pipeline we split input files into chunks which are run separately on different nodes as separate inputs for PALEOMIX and finally merge output file, it is very similar to what it done by ATLAS to process and to simulate data. We dramatically decreased the total walltime because of jobs (re)submission automation and brokering within PanDA. Using software tools developed initially for HEP and Grid can reduce payload execution time for Mammoths DNA samples from weeks to days.

  17. EVALUATION OF “SEPS” PROCEDURE IN THE MANAGEMENT OF PRIMARY VARICOSE VEINS WITH INCOMPETENT LOWER LIMB PERFORATOR

    Directory of Open Access Journals (Sweden)

    Shaik Ahmad

    2016-03-01

    Full Text Available BACKGROUND Varicose veins affect at least 1 out of 5 in the world and the cost of health care for the society is significant. In a developing country like India, study encompassing the clinical evaluation and management of lower limb varicose veins on the conventional lines seems a necessity to improve the quality care with the available resources. OBJECTIVES To study the relation between site of incompetence and complications, pattern of complications, surgical management and its outcome for lower limb varicose veins. METHODS A total 26 number of patients with primary varicose veins admitted, investigated, operated and followed up. Final outcome evaluated. All the information was taken down in the proforma, designed for the study. RESULTS In the study, it was noted that the varicose veins affect younger, adult, and middle age population. (20 to 60 years. Majority of the patients were male (84.60%. Perforator incompetence only = 42.3% (n=11. Perforator incompetence + saphenofemoral/saphenopopliteal incompetence seen in 57.7%. Long saphenous vein involvement was seen in 90.5% of the patients and both LSV and SSV involvement in 9.5%. A greater portion of the patients had combined valvular incompetence (69.56%. The mean ulcer healing time in our study was 2.8 weeks following surgery (90%. Residual incompetent perforators are seen in 7.6% (n=2. New incompetent perforators seen in 7.6% (n=2. Postoperative wound infection of the incision of SPJ ligation was seen in 3.8% (n=1 of the patients, but not the SEPS wound infection and the total complication rate was 3.85%. The mean postoperative stay for patients undergoing SEPS procedure alone was 3.6 days. The mean postoperative stay for patients who underwent perforator ligation with concomitant stripping procedure was 5 days. INTERPRETATION AND CONCLUSION Majority of the patients present with complications of varicose vein with combined valvular incompetence and surgical treatment with stripping of path

  18. 6-gingerol prevents patulin-induced genotoxicity in HepG2 cells.

    Science.gov (United States)

    Yang, Guang; Zhong, Laifu; Jiang, Liping; Geng, Chengyan; Cao, Jun; Sun, Xiance; Liu, Xiaofang; Chen, Min; Ma, Yufang

    2011-10-01

    Patulin (PAT) is a mycotoxin produced by several Penicillium, Aspergillus and Byssochlamys species. Since PAT is a potent genotoxic compound, and PAT contamination is common in fruits and fruit products, the search for newer, better agents for protection against genotoxicity of PAT is required. In this study, the chemoprotective effect of 6-gingerol against PAT-induced genotoxicity in HepG2 cells was investigated. The comet assay and micronucleus test (MNT) were used to monitor genotoxic effects. To further elucidate the underlying mechanisms, the intracellular generation of reactive oxygen species (ROS) and level of reduced glutathione (GSH) were tested. In addition, the level of oxidative DNA damage was evaluated by immunocytochemical analysis of 8-hydroxydeoxyguanosine (8-OHdG). The results showed that 6-gingerol significantly reduced the DNA strand breaks and micronuclei formation caused by PAT. Moreover, 6-gingerol effectively suppressed PAT-induced intracellular ROS formation and 8-OHdG level. The GSH depletion induced by PAT in HepG2 cells was also attenuated by 6-gingerol pretreatment. These findings suggest that 6-gingerol has a strong protective ability against the genotoxicity caused by PAT, and the antioxidant activity of 6-gingerol may play an important part in attenuating the genotoxicity of PAT. Copyright © 2011 John Wiley & Sons, Ltd.

  19. Proanthocyanidins modulate microRNA expression in human HepG2 cells.

    Directory of Open Access Journals (Sweden)

    Anna Arola-Arnal

    Full Text Available Mi(croRNAs are small non-coding RNAs of 18-25 nucleotides in length that modulate gene expression at the post-transcriptional level. These RNAs have been shown to be involved in a several biological processes, human diseases and metabolic disorders. Proanthocyanidins, which are the most abundant polyphenol class in the human diet, have positive health effects on a variety of metabolic disorders such as inflammation, obesity, diabetes and insulin resistance. The present study aimed to evaluate whether proanthocyanidin-rich natural extracts modulate miRNA expression. Using microarray analysis and Q-PCR, we investigated miRNA expression in HepG2 cells treated with proanthocyanidins. Our results showed that when HepG2 cells were treated with grape seed proanthocyanidin extract (GSPE, cocoa proanthocyanidin extract (CPE or pure epigallocatechin gallate isolated from green tea (EGCG, fifteen, six and five differentially expressed miRNAs, respectively, were identified out of 904 mRNAs. Specifically, miR-30b* was downregulated by the three treatments, and treatment with GSPE or CPE upregulated miR-1224-3p, miR-197 and miR-532-3p. Therefore, these results provide evidence of the capacity of dietary proanthocyanidins to influence microRNA expression, suggesting a new mechanism of action of proanthocyanidins.

  20. A proteomic analysis of mushroom polysaccharide-treated HepG2 cells.

    Science.gov (United States)

    Chai, Yangyang; Wang, Guibin; Fan, Lili; Zhao, Min

    2016-03-29

    The anti-tumor properties of fungal polysaccharides have gained significant recognition in Asia and tropical America. In this study, the differential expression of proteins in normal HepG2 cells and those treated with polysaccharides that had been isolated from Phellinus linteus (PL), Ganoderma lucidum (GL) and Auricularia auricula (AA) was investigated. Using two-dimensional electrophoresis (2DE), a total of 104 protein spots were determined to be overexpressed in these cells compared with noncancerous regions. A total of 59 differentially expressed proteins were identified through MALDI-TOF-MS. In addition, 400 biological processes (BP), 133 cell components (CC) and 146 molecular functions (MF) were enriched by Gene Ontology (GO) analysis, and 78 KEGG pathways were enriched by pathway enrichment. Protein-Protein Interaction (PPI) analysis demonstrated the interaction networks affected by polysaccharides in HepG2 cells. Then, DJ-1 and 14-3-3 were identified as the key proteins in the networks, and the expression of the mRNA and proteins were evaluated using Real-time quantitative PCR (qRT-PCR) and Western blotting (WB), respectively. The results were in agreement with the 2DE. These results provided information on significant proteins of hepatocellular carcinoma (HCC) and form an important basis for the future development of valuable medicinal mushroom resources.

  1. A high-throughput phenotyping procedure for evaluation of antixenosis against common cutworm at early seedling stage in soybean.

    Science.gov (United States)

    Xing, Guangnan; Liu, Kai; Gai, Junyi

    2017-01-01

    Common cutworm (CCW; Spodoptera litura Fabricius) is a major leaf-feeding pest of soybean in Asia. The previous methods of measuring antixenosis against CCW using adult plant under field or net-room conditions were time-consuming, labor-intensive and precision-inferior. To solve the problems, this study aimed at (i) establishing a high-throughput phenotyping method for evaluating antixenosis against CCW at early seedling stage, (ii) using the procedure to evaluate the antixenosis of an insect-resistant versus -susceptible germplasm population (IRSGP), (iii) validating the proposed method through comparing the results with the historical phenotypic data and phenotyping-genotyping consistency data using PAV (presence/absence variation) markers linked with the identified loci CCW-1 and CCW-2, (iv) and evaluating the efficiency of the novel method through comparisons to the previous methods. A dynamic and efficient evaluation procedure characterized with using V1 stage soybean seedlings infested with third-instar larvae in a micro-net-room in greenhouse with damaged leaf percentage (DLP) as indicator was established and designated V1TMD method. The middle term testing stage is the best dates for identifying resistant and susceptible accessions. The results from the V1TMD method were relatively stable, precise and accurate in comparison with the previous method with the detected most resistant and susceptible accessions consistent to the previous results. The DLP values differentiated obviously to coincide with the resistant and susceptible alleles of the PAV markers Gm07PAV0595 and Gm07PAV0389 tightly linked to the two resistance-related loci, CCW-1 and CCW-2, respectively, in IRSGP. Thus V1TMD is a high-throughput phenotyping method with its estimated efficiency 12 times and period shortening 4 times of those of the previous method. A dynamic and efficient V1TMD method for testing antixenosis against CCW was established, with highly resistant and highly susceptible

  2. Effects of JS-K, a novel anti-cancer nitric oxide prodrug, on gene expression in human hepatoma Hep3B cells.

    Science.gov (United States)

    Dong, Ray; Wang, Xueqian; Wang, Huan; Liu, Zhengyun; Liu, Jie; Saavedra, Joseph E

    2017-04-01

    JS-K is a novel anticancer nitric oxide (NO) prodrug effective against a variety of cancer cells, including the inhibition of AM-1 hepatoma cell growth in rats. To further evaluate anticancer effects of JS-K, human hepatoma Hep3B cells were treated with JS-K and the compound control JS-43-126 at various concentrations (0-100μM) for 24h, and cytotoxicity was determined by the MTS assay. The compound control JS-43-126 was not cytotoxic to Hep3B cells at concentrations up to 100μM, while the LC50 for JS-K was about 10μM. To examine the molecular mechanisms of antitumor effects of JS-K, Hep3B cells were treated with 1-10μM of JS-K for 24h, and then subjected to gene expression analysis via real time RT-PCR and protein immunostain via confocal images. JS-K is a GST-α targeting NO prodrug, and decreased immunostaining for GST-α was associated with JS-K treatment. JS-K activated apoptosis pathways in Hep3B cells, including induction of caspase-3, caspase-9, Bax, TNF-α, and IL-1β, and immunostaining for caspase-3 was intensified. The expressions of thrombospondin-1 (TSP-1) and the tissue inhibitors of metalloproteinase-1 (TIMP-1) were increased by JS-K at both transcript and protein levels. JS-K treatment also increased the expression of differentiation-related genes CD14 and CD11b, and depressed the expression of c-myc in Hep3B cells. Thus, multiple molecular events appear to be associated with anticancer effects of JS-K in human hepatoma Hep3B cells, including activation of genes related to apoptosis and induction of genes involved in antiangiogenesis and tumor cell migration. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  3. 20(S)-Protopanaxadiol induces apoptosis in human hepatoblastoma HepG2 cells by downregulating the protein kinase B signaling pathway.

    Science.gov (United States)

    Lu, Zeyuan; Xu, Huali; Yu, Xiaofeng; Wang, Yuchen; Huang, Long; Jin, Xin; Sui, Dayun

    2018-02-01

    Hepatoblastoma is the most common primary liver tumor for children aged S)-Protopanaxadiol (PPD) is a ginsenoside extracted from Pananx quinquefolium L ., which inhibits tumor growth in several cancer cell lines. The purpose of the present study was to assess the anticancer activities of 20(S)-PPD in human hepatoblastoma HepG2 cells. The cytotoxicity of 20(S)-PPD on HepG2 cells was evaluated using an MTT assay. Apoptosis was detected using DAPI staining and flow cytometry. The expression of apoptosis-associated proteins was identified by western blotting. The results demonstrated that 20(S)-PPD inhibited the viability of HepG2 cell in a dose and time-dependent manner. The IC 50 values were 81.35, 73.5, 48.79 µM at 24, 48 and 72 h, respectively. Topical morphological changes of apoptotic body formation following 20(S)-PPD treatment were detected by DAPI staining. The percentage of Annexin V-fluoroscein isothyiocyanate positive cells were 3.73, 17.61, 23.44 and 65.43% in HepG2 cells treated with 0, 40, 50 and 60 µM of 20(S)-PPD, respectively. Furthermore, 20(S)-PPD upregulated the expression of Bax and downregulated the expression of Bcl-2 and also activated caspases-3 and -9, and Poly [ADP-ribose] polymerase cleavage. In addition, 20(S)-PPD inhibited the phosphorylation of protein kinase B (Akt; Ser473). The results indicate that 20(S)-PPD inhibits the viability of HepG2 cells and induces apoptosis in HepG2 cells by inhibiting the phosphoinositide-3-kinase/Akt pathway.

  4. Evaluating Patient-Centered Outcomes in Clinical Trials of Procedural Sedation, Part 1 Efficacy: Sedation Consortium on Endpoints and Procedures for Treatment, Education, and Research Recommendations.

    Science.gov (United States)

    Williams, Mark R; Ward, Denham S; Carlson, Douglas; Cravero, Joseph; Dexter, Franklin; Lightdale, Jenifer R; Mason, Keira P; Miner, James; Vargo, John J; Berkenbosch, John W; Clark, Randall M; Constant, Isabelle; Dionne, Raymond; Dworkin, Robert H; Gozal, David; Grayzel, David; Irwin, Michael G; Lerman, Jerrold; O'Connor, Robert E; Pandharipande, Pratik; Rappaport, Bob A; Riker, Richard R; Tobin, Joseph R; Turk, Dennis C; Twersky, Rebecca S; Sessler, Daniel I

    2017-03-01

    The Sedation Consortium on Endpoints and Procedures for Treatment, Education, and Research, established by the Analgesic, Anesthetic, and Addiction Clinical Trial Translations, Innovations, Opportunities, and Networks public-private partnership with the US Food and Drug Administration, convened a meeting of sedation experts from a variety of clinical specialties and research backgrounds with the objective of developing recommendations for procedural sedation research. Four core outcome domains were recommended for consideration in sedation clinical trials: (1) safety, (2) efficacy, (3) patient-centered and/or family-centered outcomes, and (4) efficiency. This meeting identified core outcome measures within the efficacy and patient-centered and/or family-centered domains. Safety will be addressed in a subsequent meeting, and efficiency will not be addressed at this time. These measures encompass depth and levels of sedation, proceduralist and patient satisfaction, patient recall, and degree of pain experienced. Consistent use of the recommended outcome measures will facilitate the comprehensive reporting across sedation trials, along with meaningful comparisons among studies and interventions in systematic reviews and meta-analyses.

  5. Echocardiographic evaluation of mitral durability following valve repair in rheumatic mitral valve disease: impact of Maze procedure.

    Science.gov (United States)

    Kim, Gwan Sic; Lee, Chee Hoon; Kim, Joon Bum; Jung, Sung-Ho; Choo, Suk Jung; Chung, Cheol Hyun; Lee, Jae Won

    2014-01-01

    The data on echocardiographic evaluation of mitral durability after rheumatic mitral repair is scarce. A total of 193 patients (mean age, 39.4 ± 12.8 years; 154 females) who underwent mitral valve repair for rheumatic valve disease from 1997 to 2010 were included in the study. A Maze operation was performed in 90.3% (n = 102) of the patients with atrial fibrillation (n = 113). Survival, valve-related complications, and echocardiographic data were evaluated. Mitral regurgitation was the predominant disease in 75.6% of patients (n = 146). There was one early death (0.5%) . During the mean follow-up period of 76.7 ± 45.6 months, there were 9 late deaths and 5 mitral reoperations. Valve-related, event-free survival at 10 years was 85.5% ± 3.3%. In serial postoperative echocardiographic evaluations (mean follow-up duration, 53.7 ± 43.5 months), 40 patients showed either mitral regurgitation (>mild; n = 31) or mitral stenosis (mitral valve area ≤1.5 cm(2); n = 9). At 10 years, 66.4% ± 5.4% of the patients did not have moderate to severe mitral dysfunction. By multivariate analysis, no Maze operation for atrial fibrillation was an independent predictor of mitral dysfunctions (hazard ratio, 3.72; 95% confidence interval, 1.47-9.42; P = .005), whereas the presence of hypertension had borderline significance (hazard ratio, 3.15; 95% confidence interval, 0.96-10.38; P = .059). Although rheumatic mitral repair showed excellent long-term clinical outcomes, a significant proportion of patients experienced moderate to severe mitral dysfunctions postoperatively. Atrial fibrillation without a Maze procedure increased significantly the risks of mitral dysfunctions and adverse outcomes. Therefore, routine performance of a Maze procedure is warranted in the presence of atrial fibrillation whenever possible. Copyright © 2014 The American Association for Thoracic Surgery. Published by Mosby, Inc. All rights reserved.

  6. [Quality of life evaluation of the patients and parents satisfaction after Nuss procedure in the management of Pectus Excavatum].

    Science.gov (United States)

    Soares, Tony R; Henriques-Coelho, Tiago; Vilaça, João; Silva, Ana Raquel; Carvalho, José Luis; Correia-Pinto, Jorge

    2012-01-01

    Pectus Excavatum is the most common congenital deformity of the chest. Although Pectus Excavatum can be associated with heart or lung problems, the main indication for surgical correction of this congenital anomaly is the body image dissatisfaction. The Nuss technique is a minimally invasive procedure that allows the placement of an intrathoracic prosthetic convex bar, previously bended, which mobilize the sternum anteriorly. The aim of this study was to evaluate the degree of satisfaction of the patients and parents after the application of the Nuss technique using the Pectus Excavatum Evaluation Questionnaire. All parameters of psychosocial character - self-image, difficulties in exposing the chest, frustration, sadness, social isolation and ridicule - found a statistically significant improvement after surgical correction of Pectus Excavatum from the perspective of patients and their parents. There was not a marked improvement and consensus regarding the overall physical performance of patients. Which is understandable, since, theoretically, the physical deformity is not responsible for significant limitations on exertion. The current surgical correction of Pectus Excavatum by the Nuss technique allows obtaining aesthetic results of the chest with the resolution of the main problems with body image. Increase awareness of health issues for psychosocial and physical factors which might be involved in this kind of deformities is important, especially when there is a satisfactory solution.

  7. 3D lung spheroid cultures for evaluation of photodynamic therapy (PDT) procedures in microfluidic Lab-on-a-Chip system.

    Science.gov (United States)

    Zuchowska, Agnieszka; Jastrzebska, Elzbieta; Chudy, Michal; Dybko, Artur; Brzozka, Zbigniew

    2017-10-16

    The purpose of this paper is to present a fully integrated microchip for the evaluation of PDT procedures efficiency on 3D lung spheroid cultures. Human lung carcinoma A549 and non-malignant MRC-5 spheroids were utilized as culture models. Spheroid viability was evaluated 24 h after PDT treatment, in which 5-aminolevulinic acid (ALA) had been used as a precursor of a photosensitizer (protoporphyrin IX - PpIX). Moreover, spheroid viability over a long-term (10-day) culture was also examined. We showed that the proposed PDT treatment was toxic only for cancer spheroids. This could be because of a much-favoured enzymatic conversion of ALA to PpIX in cancer as opposed normal cells. Moreover, we showed that to obtain high effectiveness of ALA-PDT on lung cancer spheroids additional time of spheroid after light exposure was required. It was found that PDT had been effective 5 days after PDT treatment with 3 mM ALA. To the best of our knowledge this has been the first presentation of such research performed on a 3D lung spheroids culture in a microfluidic system. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Assessment of the standard forensic procedure for the evaluation of psychological injury in intimate-partner violence.

    Science.gov (United States)

    Fariña, Francisca; Arce, Ramón; Vilariño, Manuel; Novo, Mercedes

    2014-01-01

    In judicial terms, a victim refers to any person who has suffered injury arising from an action or omission of an action that constitutes an offence, and the burden of proof lies with the prosecution. A review of Spanish judicial judgements underscored that the lack of evidence of psychological injury in cases of intimate-partner violence (IPV) accounted for approximately 40% of acquittals. Thus, the Spanish standard of proof for the forensic evaluation of psychological injury i.e., the MMPI-2 and the unstructured interview were assessed in order to determine if they met the statutory requirement for the assessment of psychological injury and the differential diagnosis of feigning. The results of the comparison of 51 women victims of IPV with firm convictions against their aggressors, and 54 women mock victims of IPV showed that the F, K, Fb, Fp and Ds scales, and the F-K index discriminated significantly and with medium and large effect sizes, between adjudicated and mock victims. However, the results did not provide a valid decision criterion for forensic settings i.e., false negatives (identifying feigner as honest protocols) were not classified correctly. In conclusion, the standard forensic procedure for the evaluation of psychological injury in cases of IPV did not constitute valid proof for judges who acquitted defendants on the grounds of not proven due to the lack of evidence of psychological injury.

  9. Time- and concentration-dependent effects of resveratrol in HL-60 and HepG2 cells

    DEFF Research Database (Denmark)

    Stervbo, Ulrik; Vang, Ole; Bonnesen, Christine

    2006-01-01

    Resveratrol, a phytochemical present in grapes, has been demonstrated to inhibit tumourigenesis in animal models. However, the specific mechanism by which resveratrol exerts its anticarcinogenic effect has yet to be elucidated. In the present study, the inhibitory effects of resveratrol on cell...... proliferation and apoptosis were evaluated in the human leukaemia cell line HL-60 and the human hepatoma derived cell line HepG2. We found that after a 2 h incubation period, resveratrol inhibited DNA synthesis in a concentration-dependent manner. The IC50 value was 15 μM in both HL-60 and HepG2 cells. When...... the time of treatment was extended, an increase in IC50 value was observed; for example, at 24 h the IC50 value was 30 μM for HL-60 cells and 60 μM for HepG2 cells. Flow cytometry revealed that cells accumulated in different phases of the cell cycle depending on the resveratrol concentration. Furthermore...

  10. Synergetic cholesterol-lowering effects of main alkaloids from Rhizoma Coptidis in HepG2 cells and hypercholesterolemia hamsters.

    Science.gov (United States)

    Kou, Shuming; Han, Bing; Wang, Yue; Huang, Tao; He, Kai; Han, Yulong; Zhou, Xia; Ye, Xiaoli; Li, Xuegang

    2016-04-15

    Hyperlipidemia contributes to the progression of cardiovascular diseases. Main alkaloids from Rhizoma Coptidis including berberine (BBR), coptisine (COP), palmatine (PAL), epiberberine (EPI) and jatrorrhizine (JAT), improved dyslipidemia in hypercholesterolemic hamsters to a different degree. In this study, HepG2 cells and hypercholesterolemic hamsters were used to investigate the synergetic cholesterol-lowering efficacy of these five main alkaloids. The cellular lipid and cholesterol accumulation and in HepG2 cells were evaluated by Oil Red O staining and HPLC analysis. LDL receptor, 3-Hydroxy-3-methylglutaryl CoA reductase (HMGCR) and cholesterol 7-alpha-hydroxylase (CYP7A1) that involving cholesterol metabolism in HepG2 cells were measured by qRT-PCR, western blot and immunofluorescence analysis. The serum profiles including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c) and high-density lipoprotein cholesterol (HDL-c), as well as TC and total bile acids (TBA) of feces in hypercholesterolemic hamsters were also measured. As compared to single alkaloids, the combination of five main alkaloids (COM) reduced the lipid and cholesterol accumulation in HepG2 cells more effectively and performed an advantageous effect on controlling TC, TG, LDL-c and HDL-c in hypercholesterolemic hamsters. More effective reduction of TBA and TC levels in feces of hamsters were achieved after the administration of COM. These effects were derived from the up-regulation of LDL receptor and CYP7A1, as well as HMGCR downregulation. Our results demonstrated that COM showed a synergetic cholesterol-lowering efficacy, which was better than single alkaloids and it might be considered as a potential therapy for hypercholesterolemia. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. Synergistic effect of nutlin-3 combined with aspirin in hepatocellular carcinoma HepG2 cells through activation of Bcl-2/Bax signaling pathway.

    Science.gov (United States)

    Miao, Runchen; Xu, Xinsen; Wang, Zhixin; Liu, Sushun; Qu, Kai; Chen, Wei; Liu, Chang

    2017-12-22

    Aspirin as an antitumor drug has been studied in various malignancies with regards to its effects on apoptosis, proliferation, metastasis and senescence of tumor cells. However, the clinical application is limited by its side effects. Nutlin‑3 is a novel antitumor compound, which has not been clinically approved. The present study investigated the value of combining aspirin and nutlin‑3 on hepatocellular carcinoma (HCC) cells. MTT was performed to detect the proliferation of HepG2 cells treated with aspirin or/and nutlin‑3. Transwell invasion assays were performed to estimate the invasion ability of HepG2 cells treated with aspirin or/and nutlin‑3. Then the apoptotic analysis of HepG2 cells evaluated the synergistic effect of aspirin and nutlin‑3. Apoptosis markers, including B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), caspase‑3, caspase‑8 and caspase‑9 were estimated by western blot analysis at various time points. In addition, a Xenograft mouse model was established by infection with HepG2 cells, and aspirin and/or nutlin‑3 was administrated to verify the anti‑apoptotic effect of the two drugs in vivo. A high dose of aspirin and nutlin‑3 inhibit the proliferation and apoptosis of HepG2 cells. The antitumor effect was enhanced with the combined treatment of the two drugs, particularly in the group with a low concentration of aspirin and nutlin‑3. Nutlin‑3 was able to increase the level of Bax in HepG2 cells treated with aspirin significantly after treatment for 8 h. When treated with a low concentration of aspirin and nutlin‑3, the level of Bax in HepG2 cells was enhanced for 2 h. In the animal model, tumor volume and tumor angiogenesis were significantly decreased in combination group compared with other groups (P<0.01). Although there were side effects in the group treated with aspirin alone, no side effects were observed in the combination group. Nutlin‑3 enhanced the apoptotic effect of a low dose of

  12. Evaluation of Policy Implementation at Norm Strategy Criteria Procedure Safety Management System that Influence the Safety Culture in Building Construction, Housing, Waterworks, Road and Bridge Project in Indonesia

    OpenAIRE

    Yusuf Latief; Rossy Armyn Machfudiyanto; Brain Harryanto; Cahyadi Santoso

    2017-01-01

    Unideal Safety culture in Indonesia requires an evaluation of policy implementation refers to the construction safety regulation, Ministry Regulation 05/PRT/2014. Out of Norms, Standards, Procedures and Criteria (NSPK), we only have Norms and Procedure. As for Standard and Criteria are still being planned. This research on building, housing, water resource, roads and bridges construction at the Ministry of General Works and Housing resulted in having significant relation between safety policy...

  13. A procedure to evaluate the efficiency of surface sterilization methods in culture-independent fungal endophyte studies.

    Science.gov (United States)

    Burgdorf, R J; Laing, M D; Morris, C D; Jamal-Ally, S F

    2014-01-01

    Extraneous DNA interferes with PCR studies of endophytic fungi. A procedure was developed with which to evaluate the removal of extraneous DNA. Wheat (Triticum aestivum) leaves were sprayed with Saccharomyces cerevisiae and then subjected to physical and chemical surface treatments. The fungal ITS1 products were amplified from whole tissue DNA extractions. ANOVA was performed on the DNA bands representing S. cerevisiae on the agarose gel. Band profile comparisons using permutational multivariate ANOVA (PERMANOVA) and non-metric multidimensional scaling (NMDS) were performed on DGGE gel data, and band numbers were compared between treatments. Leaf surfaces were viewed under variable pressure scanning electron microscopy (VPSEM). Yeast band analysis of the agarose gel showed that there was no significant difference in the mean band DNA quantity after physical and chemical treatments, but they both differed significantly (p effective treatment to remove surface microbes and debris. The use of molecular and microscopy methods for the post-treatment detection of yeast inoculated onto wheat leaf surfaces demonstrated the effectiveness of the surface treatment employed, and this can assist researchers in optimizing their surface sterilization techniques in DNA-based fungal endophyte studies.

  14. A procedure to evaluate the efficiency of surface sterilization methods in culture-independent fungal endophyte studies

    Directory of Open Access Journals (Sweden)

    R.J. Burgdorf

    2014-09-01

    Full Text Available Extraneous DNA interferes with PCR studies of endophytic fungi. A procedure was developed with which to evaluate the removal of extraneous DNA. Wheat (Triticum aestivum leaves were sprayed with Saccharomyces cerevisiae and then subjected to physical and chemical surface treatments. The fungal ITS1 products were amplified from whole tissue DNA extractions. ANOVA was performed on the DNA bands representing S. cerevisiae on the agarose gel. Band profile comparisons using permutational multivariate ANOVA (PERMANOVA and non-metric multidimensional scaling (NMDS were performed on DGGE gel data, and band numbers were compared between treatments. Leaf surfaces were viewed under variable pressure scanning electron microscopy (VPSEM. Yeast band analysis of the agarose gel showed that there was no significant difference in the mean band DNA quantity after physical and chemical treatments, but they both differed significantly (p < 0.05 from the untreated control. PERMANOVA revealed a significant difference between all treatments (p < 0.05. The mean similarity matrix showed that the physical treatment results were more reproducible than those from the chemical treatment results. The NMDS showed that the physical treatment was the most consistent. VPSEM indicated that the physical treatment was the most effective treatment to remove surface microbes and debris. The use of molecular and microscopy methods for the post-treatment detection of yeast inoculated onto wheat leaf surfaces demonstrated the effectiveness of the surface treatment employed, and this can assist researchers in optimizing their surface sterilization techniques in DNA-based fungal endophyte studies.

  15. PERFORMANCE EVALUATION AND DISTRESS PREDICTION FOR EFFECTIVE RISK MANAGEMENT IN FINANCE SECTOR: AN INTEGRATED DECISION MAKING PROCEDURE

    Directory of Open Access Journals (Sweden)

    Hasan SELİM

    2017-04-01

    Full Text Available Considering its important role in the socio-economic status of the developing countries, finance sector, which is one of the core components of the service sector, is the focus of this study. The main drivers of this study are, to explore the most significant factors influencing the performance of the financial institutions in a risky environment, to evaluate the economic and financial performances using the selected factors and predict the future distress/bankruptcy possibility of the institutions by a comparative analysis employing a quantitative three-step decision making procedure. To explore the viability of the proposed approach, an up-to-date and comprehensive application on commercial banks operating in Turkish Banking sector is presented by using a wide range of financial ratios. To this aim, 44 commercial banks operating in Turkish financial sector are assessed as healthy and non-healthy by using 57 selected fundamental financial ratios to provide a comprehensive insight to the bank managers, investors, government units and rating agencies to predict the financial performances of banks and make related decisions when a risky socio-economic environment is a matter of a country.

  16. 'Stepped procedure' in laparoscopic cyst decortication during the learning period of laparoscopic surgery: Detailed evaluation of initial experiences

    Science.gov (United States)

    Emre, Huri; Turgay, Akgül; Ali, Ayyildiz; Murat, Bağcioğlu; Özgür, Yücel; Cankon, Germiyanoğlu

    2010-01-01

    BACKGROUND: We evaluated the importance and efficacy of 'stepped procedure' in laparoscopic cyst decortication as an initial experience in it. MATERIALS AND METHODS: A 36 renal cyst cases were included. The stepped retroperitonoscopic cyst excision divided into three groups. First step, doing the incisions to place the ports and expanding the retroperitoneal space with balloon distension, second step, placement of trocars and reach to the cyst, third step, aspiration and decortication of the cyst. The difficulty of the sessions was measured with the Visual Analog Scale (VAS) scoring system. Score was determined according to the difficulty of the surgical step ranging from '0' to '10', '0', too easy, '10' too difficult'. The durations were measured. One-way ANOVA test was used for statistical analysis. RESULTS: The mean age was 52.0 (20-75) years. The mean operation time was 52.0 min. The mean duration of the first step was 12.5, second, 26.0 and third, 22.5 min. The mean VAS of first step, 3.2, second, 6.0 and third, 3.6 There were only significant differences in duration time and VAS score for second step among the surgeons (Pdecortication may provide gaining experience to approach the kidney laparoscopically. The side, size and localization of cysts were not found associated with the difficulty of the method. PMID:20814509

  17. Transcrestal Sinus Lift Procedure Approaching Atrophic Maxillary Ridge: A 60-Month Clinical and Radiological Follow-Up Evaluation

    Directory of Open Access Journals (Sweden)

    G. Lo Giudice

    2015-01-01

    Full Text Available Aim. The aim of this study was to assess the success and the survival rate of dental implants placed in augmented bone after sinus lifting procedures. Material and Methods. 31 patients were mainly enrolled for a residual upper jaw crest thickness of 3 mm. CBCT scans were performed before and after the augmentation technique and at the follow-up appointments, at 3, 6, 12, 24, and up to 60 months. The follow-up examination included cumulative survival rate of implants, peri-implant marginal bone loss, and the height of sinus floor augmentation. Results. This retrospective study on 31 patients and 45 implants later inserted in a less than 3 mm crest showed excellent survival rates (99.5%, one implant was lost before loading due to an acute infection after 24 days, and two implants did not osteointegrate and were removed after 3 months. The radiological evaluation showed an average bone loss of 0.25 mm (±0.78 mm at the first follow-up appointment (3 months up to 0.30 mm (±1.28 mm after 60-month follow-up. Conclusion. In this study it was reported how even in less than 3 mm thick crest a transcrestal technique can predictably be used with a long-term clinical and radiological outcome, giving patients excellent stability of the grafted material and healthy clinical results.

  18. Comparison of atraumatic restorative treatment and conventional restorative procedures in a hospital clinic: evaluation after 30 months.

    Science.gov (United States)

    Gao, Wei; Peng, Dong; Smales, Roger J; Yip, Kevin H K

    2003-01-01

    The purpose of this study was to evaluate two glass-ionomer cements placed in the occlusal surfaces of permanent molar teeth, using two cavity preparation methods. Three dentists placed 149 restorations for 68 patients in a hospital clinic. Atraumatic restorative treatment or conventional cavity preparation methods were used for two encapsulated, high-strength conventional glass-ionomer cements: Fuji IX GP and Ketac-Molar. Non-gamma 2 amalgam alloy was used in conventional preparations for comparison. The restorative procedures were uneventful, but cavity preparations made with atraumatic restorative treatment hand instruments took approximately twice as long as did conventional rotary instrumentation. After 30 months, only one glass-ionomer cement restoration had failed. Both glass-ionomer cements showed high early losses of sealant material, but caries was not detected in the exposed fissures. Both glass-ionomer cements also showed relatively high restoration wear. At 30 months, the mean cumulative net occlusal wear was 119 +/- 12 mm for Fuji IX GP and 96 +/- 13 mm for Ketac-Molar; the difference was not statistically significant. Color matching improved significantly by 6 months; there was no significant difference in color match between the two glass-ionomer cements by 12 months. Minor surface tarnishing and marginal discrepancies were present in the amalgam restorations and increased with time. The occlusal restorations performed satisfactorily over periods of up to 30 months. However, the continued deterioration of the cements requires longer-term studies to be undertaken.

  19. Evaluation of a new experimental test procedure to more accurately determine the surface infiltration rate of permeable pavement systems

    Directory of Open Access Journals (Sweden)

    Terry Lucke

    2014-01-01

    Full Text Available Permeable pavements are specifically designed to promote the infiltration of stormwater through the paving surface in order to reduce run-off volumes and to improve water quality by removing sediment and other pollutants. However, research has shown that permeable pavements can become clogged over time and this reduces their infiltration capacity. In order to assess the infiltration of permeable pavements, a variety of infiltration test procedures have been utilised in the past. However, the results have generally been inconsistent, and have shown a large variation in the range of infiltration rates measured. This paper evaluates the performance of two new experimental test methods developed in the Netherlands to more accurately determine the surface infiltration rate of existing permeable pavement installations. The two methods were the falling head full-scale method and the constant head full-scale method. Both of the new methods involved inundating a large area of the pavement in order to determine the infiltration rate through the pavement surface. Double ring infiltrometer tests were also performed to enable a comparison of the results. The study found that the new falling head full-scale testing method produced the most accurate results.

  20. The stream of experience when watching artistic movies. Dynamic aesthetic effects revealed by the Continuous Evaluation Procedure (CEP)

    Science.gov (United States)

    Muth, Claudia; Raab, Marius H.; Carbon, Claus-Christian

    2015-01-01

    Research in perception and appreciation is often focused on snapshots, stills of experience. Static approaches allow for multidimensional assessment, but are unable to catch the crucial dynamics of affective and perceptual processes; for instance, aesthetic phenomena such as the “Aesthetic-Aha” (the increase in liking after the sudden detection of Gestalt), effects of expectation, or Berlyne's idea that “disorientation” with a “promise of success” elicits interest. We conducted empirical studies on indeterminate artistic movies depicting the evolution and metamorphosis of Gestalt and investigated (i) the effects of sudden perceptual insights on liking; that is, “Aesthetic Aha”-effects, (ii) the dynamics of interest before moments of insight, and (iii) the dynamics of complexity before and after moments of insight. Via the so-called Continuous Evaluation Procedure (CEP) enabling analogous evaluation in a continuous way, participants assessed the material on two aesthetic dimensions blockwise either in a gallery or a laboratory. The material's inherent dynamics were described via assessments of liking, interest, determinacy, and surprise along with a computational analysis on the variable complexity. We identified moments of insight as peaks in determinacy and surprise. Statistically significant changes in liking and interest demonstrated that: (i) insights increase liking, (ii) interest already increases 1500 ms before such moments of insight, supporting the idea that it is evoked by an expectation of understanding, and (iii) insights occur during increasing complexity. We propose a preliminary model of dynamics in liking and interest with regard to complexity and perceptual insight and discuss descriptions of participants' experiences of insight. Our results point to the importance of systematic analyses of dynamics in art perception and appreciation. PMID:25873907

  1. The stream of experience when watching artistic movies. Dynamic aesthetic effects revealed by the continuous evaluation procedure (CEP

    Directory of Open Access Journals (Sweden)

    Claudia eMuth

    2015-03-01

    Full Text Available Research in perception and appreciation is often focused on snapshots, stills of experience. Static approaches allow for multidimensional assessment, but are unable to catch the crucial dynamics of affective and perceptual processes; for instance, aesthetic phenomena such as the ‘Aesthetic-Aha’ (the increase in liking after the sudden detection of Gestalt, effects of expectation, or Berlyne’s idea that ‘disorientation’ with a ‘promise of success’ elicits interest. We conducted empirical studies on indeterminate artistic movies depicting the evolution and metamorphosis of Gestalt and investigated (i the effects of sudden perceptual insights on liking; that is, Aesthetic Aha-effects, (ii the dynamics of interest before moments of insight, and (iii the dynamics of complexity before and after moments of insight. Via the so-called Continuous Evaluation Procedure (CEP enabling analogous evaluation in a continuous way, participants assessed the material on two aesthetic dimensions blockwise either in a gallery or a laboratory. The material’s inherent dynamics were described via assessments of liking, interest, determinacy and surprise along with a computational analysis on the variable complexity. We identified moments of insight as peaks in determinacy and surprise. Statistically significant changes in liking and interest demonstrated that: (i insights increase liking, (ii interest already increases 1,500 ms before such moments of insight, supporting the idea that it is evoked by an expectation of understanding, and (iii insights occur during increasing complexity. We propose a preliminary model of dynamics in liking and interest with regard to complexity and perceptual insight and discuss descriptions of participants’ experiences of insight. Our results point to the importance of systematic analyses of dynamics in art perception and appreciation.

  2. INSPIRE-HEP Ergys Dona Summer Student Report 2015

    CERN Document Server

    Dona, Ergys

    2015-01-01

    INSPIRE-HEP consists of millions of bibliographic records, while new records are continuously being submitted all the time. For indexing and organising reasons, it is very important that these records contain correct and accurate metadata. The maintenance of such a large number of records is error prone, time consuming and generally difficult if performed manually. This document describes the work carried out while developing parts of the Invenio-Checker module, a piece of software that tries to automate the tasks mentioned above, as well as the abilities obtained during the development.

  3. Cuidado nutricional de pacientes con cirrosis hepática

    OpenAIRE

    Magaly Aceves-Martins

    2014-01-01

    El hígado es un órgano con funciones importantes que influyen directamente en el estado nutricional y fisiológico de las personas. En presencia de cualquier enfermedad o lesión en dicho órgano, la cirrosis hepática representa la fase final. Quienes padecen esta patología tienen disminuida la utilización y capacidad de almacenamiento de carbohidratos, además de un aumento en el catabolismo de proteínas y grasas. Esta situación, sumada a una baja ingesta y mala absorción de nutrientes, provoca ...

  4. ATLAS@Home: Harnessing Volunteer Computing for HEP

    CERN Document Server

    Cameron, David; The ATLAS collaboration

    2015-01-01

    The ATLAS collaboration has setup a volunteer computing project called ATLAS@home. Volunteers running Monte-Carlo simulation on their personal computer provide significant computing resources, but also belong to a community potentially interested in HEP. Four types of contributors have been identified, whose questions range from advanced technical details to the reason why simulation is needed, how Computing is organized and how it relates to society. The creation of relevant outreach material for simulation, event visualization and distributed production will be described, as well as lessons learned while interacting with the BOINC volunteers community.

  5. Aplastamientos vertebrales tras un trasplante hepático

    OpenAIRE

    Seral García, Belén; Bello Nicolau, M. L.; Seral Iñigo, Fernando

    1998-01-01

    En la evolución de un paciente sometido a un trasplante hepático existe una importante morbilidad con complicaciones musculoesqueléticas, especialmente debido a la osteopaina que sufren con fracturas patológicas y necrosis avasculares. Es un tema importante puesto que son pacientes jóvenes, que se encuentran sometidos a quimioterapia inmunosupresora intensiva. Además no existe una terapia profiláctica efectiva ni tratamiento de las complicaciones. Presentamos el caso de un niño de 16 años de ...

  6. The evaluation of hip muscles in patients treated with one-stage combined procedure for unilateral developmental dysplasia of the hip: part I: MRI evaluation.

    Science.gov (United States)

    Yüksel, Halil Yalçin; Yilmaz, Serdar; Aksahin, Ertuğrul; Celebi, Levent; Duran, Semra; Muratli, Hasan Hilmi; Biçimoğlu, Ali

    2009-12-01

    To assess the status of hip flexor and extensor muscles with MRI in patients with unilateral developmental dysplasia of the hip (DDH) after walking age who were treated with one-stage combined procedure. Twenty-two patients operated upon with one-stage combined procedure for unilateral DDH were included in this study. All patients underwent complete tenotomy of the iliopsoas muscle hindering open reduction. All patients showed excellent results according to the modified McKay criteria of Barrett and type 1 hips according to radiologic criteria of Severin at the latest follow-up. MRI assessment of iliopsoas, rectus femoris, tensor fasia lata, sartorius, and gluteus maximus muscles was performed and muscles of the hip that was operated upon were compared with the hip that was not. In addition, the iliopsoas muscle was examined for reattachment and the effect of reattachment was evaluated. Mean age was 154.4+/-34.6 (110 to 216) months. Mean follow-up was 112.6+/-32.0 (68 to 159) months. Reattachment of the iliopsoas was observed in 7 (32%) patients, with no significance in terms of age, postoperative follow-up period, or the duration of the postoperative period. Atrophy in the hip that was operated upon was significant in iliopsoas, rectus femoris, tensor fasia lata, and gluteus maximus muscles when compared with the hip that was not. No significance was detected in the sartorius muscle between hips that were operated upon and those that were not. Cross-sectional areas of tensor fascia lata, rectus femoris, sartorius, and gluteus maximus muscles were not significantly different between patients with and without reattachment of iliopsoas. The width of the iliopsoas muscle was significantly lowered in patients without reattachment. Patients with DDH treated with combined procedures including complete iliopsoas tenotomy do not have hip muscles similar to hip muscles that have not been operated upon, despite excellent radiologic and clinical results. These patients can be

  7. Evaluation of different procedure involved in the Transcranial Direct Current Stimulation (tDCS technique experimental application

    Directory of Open Access Journals (Sweden)

    Lauren Naomi Spezia Adachi

    2017-07-01

    Full Text Available Introduction: The transcranial direct current stimulation (tDCS is a non-invasive technique, which induces neuroplastic changes in the central nervous system of animals and humans. Furthermore, tDCS has been suggested as a therapeutic tool for pain management. The aim of this study was to standardize a non-invasive tDCS technique indexed by the nociceptive response of rats submitted to different conditions necessary to the tDCS application. Method: 60-day-old male Wistar rats (n=65, divided into 6 groups: control(C; non-active sham (NAS; active-sham (AS; active-sham restrained (ASR; non-active sham restrained (NASR; active tDCS treatment. Animals received treatment during 30 seconds (sham-active or 20 minutes (restraint and tDCS/8 days. Nociceptive threshold was assessed by Hot Plate test at baseline, immediately and 24h after the first session, immediately and 24h after the last session. Variance analysis of repeated measurements followed by Bonferroni was performed for intra-group comparison. Results: Physical restraint and 30 seconds stimulation (sham-tDCS increased pain sensitivity (P≤0.05, and tDCS treatment was able to prevent the thermal hyperalgesia. Our original tDCS montage is similar to that used in the procedure with humans, because it is not an invasive technique. The electrodes are positioned on the head, and the animals are immobilized during the 20-minute treatment. As this procedure could involve behavior and neurochemical alterations due to stress induced by restriction (thus, it creates a research bias, we hypothesized that a 30-second electrical stimulus application (sham-tDCS and the physical restriction used during tDCS treatment might alter nociceptive response in rats. Conclusion: There are methodological limitations in the present tDCS-technique. Although active-tDCS treatment is able to prevent these harmful effects, interference of these factors has to be considered during the results’ analysis. Future adaptations

  8. Improvement and Evaluation of Copper Oxidation Experimental Procedure for the Introduction of the Law of Definite Proportion

    Science.gov (United States)

    Yamashita, Shuichi; Kashiwaguma, Yasuyuki; Hayashi, Hideko; Pietzner, Verena

    2017-01-01

    In science classes, students usually learn about the law of definite proportions by the oxidation of copper. However, common procedures usually do not lead to proper results. This leads to confusion among the students because their experimental results do not fit to the theoretical values. Therefore, we invented a new procedure for this experiment…

  9. An Evaluation of Constant Time Delay and Simultaneous Prompting Procedures in Skill Acquisition for Young Children with Autism

    Science.gov (United States)

    Brandt, Julie A. Ackerlund; Weinkauf, Sara; Zeug, Nicole; Klatt, Kevin P.

    2016-01-01

    Previous research has shown that various prompting procedures are effective in teaching skills to children and adults with developmental disabilities. Simultaneous prompting includes proving a prompt immediately following an instruction; whereas constant time-delay procedures include a set time delay (i.e., 5 s or 10 s) prior to delivering a…

  10. Involvement of endoplasmic reticulum and autophagy in microcystin-LR toxicity in Vero-E6 and HepG2 cell lines.

    Science.gov (United States)

    Menezes, Carina; Alverca, Elsa; Dias, Elsa; Sam-Bento, Filomena; Pereira, Paulo

    2013-02-01

    This work investigates the involvement of the endoplasmic reticulum (ER) and autophagy in microcystin-LR (MCLR) toxicity in Vero-E6 and HepG2 cell lines. Additionally, morphological alterations induced by MCLR in lysosomes and mitochondria were studied. Cytotoxicity evaluation showed that pure MCLR and MCLR from LMECYA110 extract induce concentration dependent viability decays after 24h exposure. HepG2 cells showed an increased sensitivity to MCLR than Vero cells, with lower cytotoxic thresholds and EC(50) values. Conversely, LC3B immunofluorescence showed that autophagy is triggered in both cell lines as a survival response to low MCLR concentrations. Furthermore, MCLR induced a MCLR concentration-dependent decrease of GRP94 expression in HepG2 cells while in Vero cells no alteration was observed. This suggests the involvement of the ER in HepG2 apoptosis elicited by MCLR, while in Vero cells ER destructuration could be a consequence of cytoskeleton inflicted damages. Additionally, in both cell lines, lysosomal destabilization preceded mitochondrial impairment which occurred at high toxin concentrations. Although not an early cellular target of MCLR, mitochondria appears to serve as central mediators of different signaling pathways elicited by the organelles involved in MCLR toxicity. As a result, kidney and hepatic cell lines exhibit cell type and dose-dependent mechanisms to overcome MCLR toxicity. Copyright © 2012 Elsevier Ltd. All rights reserved.

  11. Apoptotic and Inhibitory Effects on Cell Proliferation of Hepatocellular Carcinoma HepG2 Cells by Methanol Leaf Extract of Costus speciosus

    Directory of Open Access Journals (Sweden)

    Sandhya V. G. Nair

    2014-01-01

    Full Text Available Costus speciosus is a medicinal plant commonly known as wild ginger distributed in South and Southeast Asian countries. Leaves of this plant are used for ayurvedic treatment regimes in malignancies and mental illness. Rhizome extract from the plant is used to treat malignancies, pneumonia, urinary disorders, jaundice, rheumatism, and diabetes. The goal of this study was to investigate the effects of methanol extract of leaves of C. speciosus on the growth of human hepatocellular carcinoma (HepG2 cells and understand possible mechanisms of its action. Viability of HepG2 cells were measured by MTS assay after 24 h and 48 h treatment with extracts of 1, 10, 50, 100, and 200 μg/mL concentrations. Cell cycle analysis and apoptosis were evaluated by flow cytometry and caspase-3 induction. HepG2 cells treated with 100 μg/mL methanol leaf extract for 24 h displayed a significant reduction in cell viability (P≤0.05. The methanol extract perturbed cell cycle progression, modulated cell cycle and regulated, signal molecules were involved in induction of apoptosis in HepG2 cells. Our findings indicate that phytochemicals of leaves of C. speciosus shows potential for natural therapeutic product development for hepatocellular carcinoma. This is the first report to demonstrate in vitro anticancer activity of leaf extract of C. speciosus in relation to liver cancer.

  12. Potassium Dichromate Induced Cytotoxicity, Genotoxicity and Oxidative Stress in Human Liver Carcinoma (HepG2 Cells

    Directory of Open Access Journals (Sweden)

    Diahanna Hackett

    2009-02-01

    Full Text Available Chromium is a widespread industrial waste. The soluble hexavalent chromium Cr (VI is an environmental contaminant widely recognized to act as a carcinogen, mutagen and teratogen towards humans and animals. The fate of chromium in the environment is dependent on its oxidation state. Hexavalent chromium primarily enters the cells and undergoes metabolic reduction to trivalent chromium, resulting in the formation of reactive oxygen species together with oxidative tissue damage and a cascade of cellular events. However, the results from in vitro studies are often conflicting. The aim of this study was to develop a model to establish relationships between cytotoxicity, genotoxicity and oxidative stress, in human liver carcinoma [HepG2] cells exposed to potassium dichromate. HepG2 cells were cultured following standard protocols and exposed to various concentrations [0-50 µM] of potassium dichromate [K2Cr2O7]. Following exposure to the toxic metal, the MTT assay was performed to assess the cytotoxicity, the thiobarbituric acid test to evaluate the degree of lipid peroxidation as an indicator of oxidative stress and the alkaline comet assay was used to assess DNA damage to study genotoxicity. The results of the study indicated that potassium dichromate was cytotoxic to HepG2 cells. The LD50 values of 8.83 ± 0.89 µg/ml, 6.76 ± 0.99 µg/ml, respectively, for cell mortality at 24 and 48 hrs were observed, indicating a dose- and time-dependent response with regard to the cytotoxic effects of potassium dichromate. A statistically significant increase in the concentration of malondialdehyde [MDA], an indicator of lipid peroxidation, was recorded in exposed cells [15.9 – 69.9 µM] compared to control [13 µM]. Similarly, a strong dose-response relationship (p<0.05 was also obtained with respect to potassium dichromate induced DNA damage (comet assay in HepG2 cells exposed [3.16 ± 0.70 – 24.84 ± 1.86 microns – mean comet tail length]; [12.4 ± 1

  13. Potassium dichromate induced cytotoxicity, genotoxicity and oxidative stress in human liver carcinoma (HepG2) cells.

    Science.gov (United States)

    Patlolla, Anita K; Barnes, Constance; Hackett, Diahanna; Tchounwou, Paul B

    2009-02-01

    Chromium is a widespread industrial waste. The soluble hexavalent chromium Cr (VI) is an environmental contaminant widely recognized to act as a carcinogen, mutagen and teratogen towards humans and animals. The fate of chromium in the environment is dependent on its oxidation state. Hexavalent chromium primarily enters the cells and undergoes metabolic reduction to trivalent chromium, resulting in the formation of reactive oxygen species together with oxidative tissue damage and a cascade of cellular events. However, the results from in vitro studies are often conflicting. The aim of this study was to develop a model to establish relationships between cytotoxicity, genotoxicity and oxidative stress, in human liver carcinoma [HepG2] cells exposed to potassium dichromate. HepG2 cells were cultured following standard protocols and exposed to various concentrations [0-50 microM] of potassium dichromate [K2Cr2O7]. Following exposure to the toxic metal, the MTT assay was performed to assess the cytotoxicity, the thiobarbituric acid test to evaluate the degree of lipid peroxidation as an indicator of oxidative stress and the alkaline comet assay was used to assess DNA damage to study genotoxicity. The results of the study indicated that potassium dichromate was cytotoxic to HepG2 cells. The LD(50) values of 8.83 +/- 0.89 microg/ml, 6.76 +/- 0.99 microg/ml, respectively, for cell mortality at 24 and 48 hrs were observed, indicating a dose- and time-dependent response with regard to the cytotoxic effects of potassium dichromate. A statistically significant increase in the concentration of malondialdehyde [MDA], an indicator of lipid peroxidation, was recorded in exposed cells [15.9 - 69.9 microM] compared to control [13 microM]. Similarly, a strong dose-response relationship (pDNA damage (comet assay) in HepG2 cells exposed [3.16 +/- 0.70 - 24.84 +/- 1.86 microns - mean comet tail length]; [12.4 +/- 1.45% - 76 +/- 1.49%-% tail DNA] to potassium dichromate than control [3

  14. Polyphenols from the extract and fraction of T. indica seeds protected HepG2 cells against oxidative stress.

    Science.gov (United States)

    Razali, Nurhanani; Mat Junit, Sarni; Ariffin, Azhar; Ramli, Nur Siti Fatimah; Abdul Aziz, Azlina

    2015-12-18

    Tamarindus indica L. (T. indica) or locally known as "asam jawa" belongs to the family Leguminosae. T. indica seeds as by-products from the fruits were previously reported to contain high polyphenolic content. However, identification of their bioactive polyphenols using recent technologies is less well researched but nonetheless important. Hence, it was the aim of this study to provide further information on the polyphenolic content and antioxidant activities as well as to identify and quantify its bioactive polyphenols. T. indica seeds were extracted with methanol and were then fractionated with different compositions of hexane, ethyl acetate and methanol. Polyphenolic contents were measured using Folin-Ciocalteu assay while antioxidant activities were measured using DPPH radical scavenging and ferric reducing (FRAP) activities. The cytotoxic activities of the crude extract and the active fraction were evaluated in HepG2 cells using MTT assay. The cells were then pre-treated with the IC20 concentrations and induced with H2O2 before measuring their cellular antioxidant activities including FRAP, DPPH, lipid peroxidation, ROS generation and antioxidant enzymes, SOD, GPx and CAT. Analyses of polyphenols in the crude extract and its active fraction were done using UHPLC and NMR. Amongst the 7 isolated fractions, fraction F3 showed the highest polyphenolic content and antioxidant activities. When HepG2 cells were treated with fraction F3 or the crude extract, the former demonstrated higher antioxidant activities. F3 also showed stronger inhibition of lipid peroxidation and ROS generation, and enhanced activities of SOD, GPx and CAT of HepG2 cells following H2O2-induced oxidative damage. UHPLC analyses revealed the presence of catechin, procyanidin B2, caffeic acid, ferulic acid, chloramphenicol, myricetin, morin, quercetin, apigenin and kaempferol, in the crude seed extract of T. indica. UHPLC and NMR analyses identified the presence of caffeic acid in fraction F3. Our

  15. Big Data in HEP: A comprehensive use case study

    Energy Technology Data Exchange (ETDEWEB)

    Gutsche, Oliver [Fermilab; Cremonesi, Matteo [Fermilab; Elmer, Peter [Princeton U.; Jayatilaka, Bo [Fermilab; Kowalkowski, Jim [Fermilab; Pivarski, Jim [Princeton U.; Sehrish, Saba [Fermilab; Mantilla Surez, Cristina [Johns Hopkins U.; Svyatkovskiy, Alexey [Princeton U.; Tran, Nhan [Fermilab

    2017-01-31

    Experimental Particle Physics has been at the forefront of analyzing the worlds largest datasets for decades. The HEP community was the rst to develop suitable software and computing tools for this task. In recent times, new toolkits and systems collectively called Big Data technologies have emerged to support the analysis of Petabyte and Exabyte datasets in industry. While the principles of data analysis in HEP have not changed (ltering and transforming experiment-specic data formats), these new technologies use dierent approaches and promise a fresh look at analysis of very large datasets and could potentially reduce the time-to-physics with increased interactivity. In this talk, we present an active LHC Run 2 analysis, searching for dark matter with the CMS detector, as a testbed for Big Data technologies. We directly compare the traditional NTuple-based analysis with an equivalent analysis using Apache Spark on the Hadoop ecosystem and beyond. In both cases, we start the analysis with the ocial experiment data formats and produce publication physics plots. We will discuss advantages and disadvantages of each approach and give an outlook on further studies needed. 1.

  16. Inferring Toxicological Responses of HepG2 Cells from ...

    Science.gov (United States)

    Understanding the dynamic perturbation of cell states by chemicals can aid in for predicting their adverse effects. High-content imaging (HCI) was used to measure the state of HepG2 cells over three time points (1, 24, and 72 h) in response to 976 ToxCast chemicals for 10 different concentrations (0.39-200µM). Cell state was characterized by p53 activation (p53), c-Jun activation (SK), phospho-Histone H2A.x (OS), phospho-Histone H3 (MA), alpha tubulin (Mt), mitochondrial membrane potential (MMP), mitochondrial mass (MM), cell cycle arrest (CCA), nuclear size (NS) and cell number (CN). Dynamic cell state perturbations due to each chemical concentration were utilized to infer coarse-grained dependencies between cellular functions as Boolean networks (BNs). BNs were inferred from data in two steps. First, the data for each state variable were discretized into changed/active (> 1 standard deviation), and unchanged/inactive values. Second, the discretized data were used to learn Boolean relationships between variables. In our case, a BN is a wiring diagram between nodes that represent 10 previously described observable phenotypes. Functional relationships between nodes were represented as Boolean functions. We found that inferred BN show that HepG2 cell response is chemical and concentration specific. We observed presence of both point and cycle BN attractors. In addition, there are instances where Boolean functions were not found. We believe that this may be either

  17. Applying deep neural networks to HEP job classification

    Science.gov (United States)

    Wang, L.; Shi, J.; Yan, X.

    2015-12-01

    The cluster of IHEP computing center is a middle-sized computing system which provides 10 thousands CPU cores, 5 PB disk storage, and 40 GB/s IO throughput. Its 1000+ users come from a variety of HEP experiments. In such a system, job classification is an indispensable task. Although experienced administrator can classify a HEP job by its IO pattern, it is unpractical to classify millions of jobs manually. We present how to solve this problem with deep neural networks in a supervised learning way. Firstly, we built a training data set of 320K samples by an IO pattern collection agent and a semi-automatic process of sample labelling. Then we implemented and trained DNNs models with Torch. During the process of model training, several meta-parameters was tuned with cross-validations. Test results show that a 5- hidden-layer DNNs model achieves 96% precision on the classification task. By comparison, it outperforms a linear model by 8% precision.

  18. Big Data in HEP: A comprehensive use case study

    Science.gov (United States)

    Gutsche, Oliver; Cremonesi, Matteo; Elmer, Peter; Jayatilaka, Bo; Kowalkowski, Jim; Pivarski, Jim; Sehrish, Saba; Mantilla Surez, Cristina; Svyatkovskiy, Alexey; Tran, Nhan

    2017-10-01

    Experimental Particle Physics has been at the forefront of analyzing the worlds largest datasets for decades. The HEP community was the first to develop suitable software and computing tools for this task. In recent times, new toolkits and systems collectively called Big Data technologies have emerged to support the analysis of Petabyte and Exabyte datasets in industry. While the principles of data analysis in HEP have not changed (filtering and transforming experiment-specific data formats), these new technologies use different approaches and promise a fresh look at analysis of very large datasets and could potentially reduce the time-to-physics with increased interactivity. In this talk, we present an active LHC Run 2 analysis, searching for dark matter with the CMS detector, as a testbed for Big Data technologies. We directly compare the traditional NTuple-based analysis with an equivalent analysis using Apache Spark on the Hadoop ecosystem and beyond. In both cases, we start the analysis with the official experiment data formats and produce publication physics plots. We will discuss advantages and disadvantages of each approach and give an outlook on further studies needed.

  19. Evaluation of methods to produce an image library for automatic patient model localization for dose mapping during fluoroscopically guided procedures

    Science.gov (United States)

    Kilian-Meneghin, Josh; Xiong, Z.; Rudin, S.; Oines, A.; Bednarek, D. R.

    2017-03-01

    The purpose of this work is to evaluate methods for producing a library of 2D-radiographic images to be correlated to clinical images obtained during a fluoroscopically-guided procedure for automated patient-model localization. The localization algorithm will be used to improve the accuracy of the skin-dose map superimposed on the 3D patient- model of the real-time Dose-Tracking-System (DTS). For the library, 2D images were generated from CT datasets of the SK-150 anthropomorphic phantom using two methods: Schmid's 3D-visualization tool and Plastimatch's digitally-reconstructed-radiograph (DRR) code. Those images, as well as a standard 2D-radiographic image, were correlated to a 2D-fluoroscopic image of a phantom, which represented the clinical-fluoroscopic image, using the Corr2 function in Matlab. The Corr2 function takes two images and outputs the relative correlation between them, which is fed into the localization algorithm. Higher correlation means better alignment of the 3D patient-model with the patient image. In this instance, it was determined that the localization algorithm will succeed when Corr2 returns a correlation of at least 50%. The 3D-visualization tool images returned 55-80% correlation relative to the fluoroscopic-image, which was comparable to the correlation for the radiograph. The DRR images returned 61-90% correlation, again comparable to the radiograph. Both methods prove to be sufficient for the localization algorithm and can be produced quickly; however, the DRR method produces more accurate grey-levels. Using the DRR code, a library at varying angles can be produced for the localization algorithm.

  20. Advanced human-system interface design review guideline. Evaluation procedures and guidelines for human factors engineering reviews

    Energy Technology Data Exchange (ETDEWEB)

    O`Hara, J.M.; Brown, W.S. [Brookhaven National Lab., Upton, NY (United States); Baker, C.C.; Welch, D.L.; Granda, T.M.; Vingelis, P.J. [Carlow International Inc., Falls Church, VA (United States)

    1994-07-01

    Advanced control rooms will use advanced human-system interface (HSI) technologies that may have significant implications for plant safety in that they will affect the operator`s overall role in the system, the method of information presentation, and the ways in which operators interact with the system. The U.S. Nuclear Regulatory Commission (NRC) reviews the HSI aspects of control rooms to ensure that they are designed to good human factors engineering principles and that operator performance and reliability are appropriately supported to protect public health and safety. The principal guidance available to the NRC, however, was developed more than ten years ago, well before these technological changes. Accordingly, the human factors guidance needs to be updated to serve as the basis for NRC review of these advanced designs. The purpose of this project was to develop a general approach to advanced HSI review and the human factors guidelines to support. NRC safety reviews of advanced systems. This two-volume report provides the results of the project. Volume I describes the development of the Advanced HSI Design Review Guideline (DRG) including (1) its theoretical and technical foundation, (2) a general model for the review of advanced HSIs, (3) guideline development in both hard-copy and computer-based versions, and (4) the tests and evaluations performed to develop and validate the DRG. Volume I also includes a discussion of the gaps in available guidance and a methodology for addressing them. Volume 2 provides the guidelines to be used for advanced HSI review and the procedures for their use.

  1. Suppressing Pitx2 inhibits proliferation and promotes differentiation of iHepSCs.

    Science.gov (United States)

    Chen, Fei; Yao, Hao; Wang, Minjun; Yu, Bing; Liu, Qinggui; Li, Jianxiu; He, Zhiying; Hu, Yi-Ping

    2016-11-01

    Induced hepatic stem cells (iHepSCs) have great potential as donors for liver cell therapy due to their abilities for self-renewal and bi-potential differentiation. However, the molecular mechanism regulating proliferation and differentiation of iHepSCs is poorly understood. In this study, we provide evidence that the homeodomain transcription factor, Pitx2, is essential to maintain iHepSCs stem cell characteristics. Suppressing Pitx2 expression in iHepSCs by lentivirus mediated specific shRNA markedly reduced the expression of the hepatic stem cell-associated genes (Lgr5, EpCAM, and Sox9) with concomitant inhibition of proliferation by blocking the G1/S phase transition, and these phenotypic changes were reversed upon re-expression of Pitx2. Pitx2 knockdown also resulted in up-regulation of the p53-induced Cdk inh