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Sample records for european flounder platichthys

  1. Adaptive differences in gene expression in European flounder ( Platichthys flesus )

    DEFF Research Database (Denmark)

    Larsen, Peter Foged; Eg Nielsen, Einar; Williams, T.D.

    2007-01-01

    Population structure was previously believed to be very limited or absent in classical marine fishes, but recently, evidence of weakly differentiated local populations has been accumulating using noncoding microsatellite markers. However, the evolutionary significance of such minute genetic...... differences remains unknown. Therefore, in order to elucidate the relationship between genetic markers and adaptive divergence among populations of marine fishes, we combined cDNA microarray and microsatellite analysis in European flounders (Platichthys flesus). We demonstrate that despite extremely low...

  2. Cryptic behaviour of juvenile turbot Psetta maxima L. and European flounder Platichthys flesus L

    DEFF Research Database (Denmark)

    Kristensen, Louise Dahl; Sparrevohn, Claus Reedtz; Christensen, Jens Tang

    2014-01-01

    The aim of this study was to examine the burying behaviour of hatchery-reared European flounder Platichthys flesus and turbot Psetta maxima, and whether conditioning on a sandy substrate would improve burying efficiency. Both species buried shortly after release on a sandy substrate. However...... and turbot with a tendency for larger fish to bury more efficiently than smaller fish, despite previous conditioning. Size at settlement was found to be >2 cm for flounder and >3 cm for turbot....

  3. Comparisons of liver proteomes in the European flounder Platichthys flesus from three contrasted estuaries

    Science.gov (United States)

    Galland, Claire; Dupuy, Célie; Capitaine, Carole; Auffret, Michel; Quiniou, Louis; Laroche, Jean; Pichereau, Vianney

    2013-01-01

    Estuaries are important areas highly susceptible to anthropogenic degradations like pollution. Estuarine species have thus to cope with many types of constraints depending on the estuaries' characteristics. The European flounder Platichthys flesus is considered as a sentinel species for the monitoring of estuarine water quality. In this study, juvenile flounders (0+ group) were sampled from three contrasted Channel estuaries, i.e. the Seine, the Canche and the Tamar, and we characterized their liver proteomes by using a two-dimensional electrophoresis based proteomic approach. We showed that 27 protein spots differentially accumulated between the 3 populations. Six of these proteins were identified by MALDI TOF-TOF mass spectrometry. Flounders from the Seine and from the Tamar, two highly polluted estuaries, displayed common differences, i.e. an increase of the energetic- and the glutathione-metabolism. The most accumulated protein in the Seine's samples (6.7-fold) was a Vitelline Membrane Outer layer protein 1 homolog, suggesting oogenesis deregulation in these juvenile (sexually immature) flounders. Future works applying this kind of proteomic approach on flounders experimentally exposed to conditions that mimic environmental constraints will help to better understand the significance of these environmental proteomic signatures.

  4. Cryptic behaviour of juvenile turbot Psetta maxima L. and European flounder Platichthys flesus L

    DEFF Research Database (Denmark)

    Kristensen, Louise Dahl; Sparrevohn, Claus Reedtz; Christensen, Jens Tang

    2014-01-01

    The aim of this study was to examine the burying behaviour of hatchery-reared European flounder Platichthys flesus and turbot Psetta maxima, and whether conditioning on a sandy substrate would improve burying efficiency. Both species buried shortly after release on a sandy substrate. However...

  5. Early contamination of European flounder (Platichthys flesus) by PCDD/Fs and dioxin-like PCBs in European waters.

    Science.gov (United States)

    Nunes, Margarida; Martinho, Filipe; Vernisseau, Anaïs; Marchand, Philippe; Le Bizec, Bruno; van der Veer, Henk W; Cabral, Henrique N; Ramos, Fernando; Pardal, Miguel A

    2014-08-15

    Contamination levels and profiles of 7 polychlorinated-p-dioxins, 10 polychlorinated furans (PCDD/Fs) and 12 dioxin-like polychlorinated biphenyls (dl-PCBs) were investigated in juvenile European flounder (Platichthys flesus) captured in different nursery areas in the northeastern Atlantic coast across its geographical distribution range. The toxic equivalent concentrations (WHO-TEQfish) were also determined in order to evaluate which P. flesus population was more exposed to dioxin-like toxicity. Juveniles caught in the Sørfjord (Norway) showed the lowest WHO-TEQfish concentration (0.052 pg WHO-TEQfish g(-1)wet weight) whereas the highest value was observed in fish from the Wadden Sea (The Netherlands; 0.291 pg WHO-TEQfish g(-1)ww), mainly due to the greater contribution of 2,3,7,8-tetrachlorodibenzo-p-dioxin, the most toxic congener. Nonetheless, when comparing the results with existent tissue residue-based toxicity benchmarks, no adverse effects resulting from PCDD/Fs and dl-PCBs are expected to occur in flounder from the studied systems. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. Binding and movement of silver in the intestinal epithelium of a marine teleost fish, the European flounder (Platichthys flesus)

    DEFF Research Database (Denmark)

    Hogstrand, C.; Wood, C. M.; Bury, N.R.

    2002-01-01

    mixtures at concentrations ranging from 1.6 to 950 nM total silver. Appearance of 110mAg was analysed in mucosal scrapings, muscle layers, and in the plasma saline on the serosal side of the intestine. The latter represented uptake into blood and other extra-intestinal compartments. Mucosal scrapings......The intestine has been indicated as a site of waterborne silver toxicity in marine fish and chronic effects at the intestine have been observed at concentrations far below acutely toxic level. Thus, models of silver toxicity to marine fish need to consider the intestine as a biotic ligand....... The present study characterises binding of silver to the intestine of the European flounder (Platichthys flesus). Everted intestinal sacks were prepared and submersed in a solution mimicking the intestinal fluid of the fish at the acclimation salinity (21‰). Silver was added as 110mAgNO3 or 110mAgNO3/AgNO3...

  7. Physiological response in the European flounder (Platichthys flesus) to variable salinity and oxygen conditions

    DEFF Research Database (Denmark)

    Lundgreen, Kim; Kiilerich, Pia; Tipsmark, Christian Kølbæk

    2008-01-01

    Physiological mechanisms involved in acclimation to variable salinity and oxygen levels and their interaction were studied in European flounder. The fish were acclimated for two weeks to freshwater (1 ‰ salinity), brackish water (11 ‰) or full strength seawater (35 ‰) under normoxic conditions...... (water Po2 = 158 mmHg) and then subjected to 48 h of continued normoxia or hypoxia at a level (Po2 = 54 mmHg) close to but above the critical Po2. Plasma osmolality, [Na+] and [Cl-] increased with increasing salinity, but the rises were limited, reflecting an effective extracellular osmoregulation....... Muscle water content was the same at all three salinities, indicating complete cell volume regulation. Gill Na+/K+-ATPase activity did not change with salinity, but hypoxia caused a 25 % decrease in branchial Na+/K+-ATPase activity at all three salinities. Furthermore, hypoxia induced a significant...

  8. Histopathological effects of chronic aqueous exposure to bis(tri-n-butyltin)oxide (TBTO) to environmentally relevant concentrations reveal thymus atrophy in European flounder (Platichthys flesus)

    Energy Technology Data Exchange (ETDEWEB)

    Grinwis, G.C.M., E-mail: g.c.m.grinwis@uu.n [Department of Pathobiology, Faculty of Veterinary Medicine University of Utrecht, P.O. Box 80.158, 3508 TD Utrecht (Netherlands); Wester, P.W. [Laboratory for Toxicology, Pathology and Genetics, National Institute for Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven (Netherlands); Vethaak, A.D. [Deltares, Unit Coastal and Marine Systems, P.O. Box 177, 2600 MH Delft (Netherlands)

    2009-10-15

    Although the use of tributyltin in antifouling paints has been banned, this compound is still a serious pollutant of the marine environment. This paper describes a unique study in which European flounder (Platichthys flesus) were chronically (8 months) exposed to bis(tri-n-butyltin)oxide (TBTO) in the water under controlled laboratory conditions. Residue levels in selected tissues (liver, muscle) and general health status indices were measured and the effects on several organs (gills, liver, mesonephros, ovary/testis, spleen, and gastrointestinal tract) were examined histopathologically. Additionally, morphometric analysis of the thymus was performed. The major finding is that exposure of flounder to 5 mug TBTO/l over a period of 8 months, resulting in body burdens comparable to high field levels, induced significant reduction of thymus volume, possibly affecting immunocompetence of the animals. Chronic exposure of European flounder to tributyltin is therefore likely to affect the general health status of this species in heavily polluted aquatic environments. - Current levels of tributyltin antifouling contamination are still likely to affect the general health status of estuarine flatfish in heavily polluted environments.

  9. Early contamination of European flounder (

    NARCIS (Netherlands)

    Nunes, M.; Martinho, F.; Vernisseau, A.; Marchand, P.; Le Bizec, B.; van der Veer, H.W.; Cabral, H.N.; Ramos, F.; Pardal, M.A.

    2014-01-01

    Contamination levels and profiles of 7 polychlorinated-p-dioxins, 10 polychlorinated furans (PCDD/Fs) and 12 dioxin-like polychlorinated biphenyls (dl-PCBs) were investigated in juvenile European flounder (Platichthys flesus) captured in different nursery areas in the northeastern Atlantic coast

  10. Immigration and early life stages recruitment of the European flounder (Platichthys flesus) to an estuarine nursery: The influence of environmental factors

    Science.gov (United States)

    Amorim, Eva; Ramos, Sandra; Elliott, Michael; Bordalo, Adriano A.

    2016-01-01

    Connectivity between coastal spawning grounds and estuarine nurseries is a critical step in the life cycle of many fish species. Larval immigration and transport-associated physical-biological processes are determinants of recruitment success to nursery areas. The recruitment of the European flounder, Platichthys flesus, to estuarine nurseries located at the southern edge of the species distribution range, has been usually investigated during its juvenile stages, while estuarine recruitment during the earlier planktonic life stage remains largely unstudied. The present study investigated the patterns of flounder larval recruitment and the influence of environmental factors on the immigration of the early life stages to the Lima estuary (NW Portugal), integrating data on fish larvae and post-settlement individuals (< 50 mm length), collected over 7 years. Late-stage larvae arrived at the estuary between February and July and peak abundances were observed in April. Post-settlement individuals (< 50 mm) occurred later between April and October, whereas newly-settled ones (< 20 mm) were found only in May and June. Variables associated with the spawning, survival and growth of larvae in the ocean (sea surface temperature, chlorophyll a and inland hydrological variables) were the major drivers of flounder occurrence in the estuarine nursery. Although the adjacent coastal area is characterized by a current system with strong seasonality and mesoscale variability, we did not identify any influence of variables related with physical processes (currents and upwelling) on the occurrence of early life stages in the estuary. A wider knowledge on the influence of the coastal circulation variability and its associated effects upon ocean-estuarine connectivity is required to improve our understanding of the population dynamics of marine spawning fish that use estuarine nurseries.

  11. A combined measurement of metal bioaccumulation and condition indices in juvenile European flounder, Platichthys flesus, from European estuaries.

    Science.gov (United States)

    Kerambrun, E; Henry, F; Cornille, V; Courcot, L; Amara, R

    2013-04-01

    Condition indices and metal bioaccumulation of early life stages of juvenile flounder (5-10 cm) were determined in three anthropogenic estuaries (the Scheldt, Seine and Loire) and compared to a reference site (the Canche). Significant correlations were found between metal concentrations in sediment and (i) fish liver for Cd, Cu, Ni, Pb, V and Zn and (ii) fish gills for Cd and Mn. Metal accumulation in juvenile flounder from the three anthropogenic estuaries coincided with significantly lower Fulton's K indices (from 0.99 ± 0.03 to 1.06 ± 0.01 mg mm(-3)) compared to those from the Canche estuary (from 1.02 ± 0.01 to 1.13 ± 0.01 mg mm(-3)). This discrepancy in fish condition index increased with fish size and therefore, strongly depends on the time juvenile spend in estuary. Muscle lipid contents and Triacylglycerol to Sterol ratios were significantly lower in fish collected in the Scheldt (lipid content: 21.3 ± 3.6%), Seine (17.9 ± 19.8%) and Loire (19.5 ± 2.4%) estuaries compared to those originating from the Canche (38.3 ± 4.6%). This study highlights that combined measures of both fish metal contents and condition indices gives a relevant assessment of juvenile fish health growing in anthropogenic estuaries. Copyright © 2012 Elsevier Ltd. All rights reserved.

  12. Clonal xenobiotic resistance during pollution-induced toxic injury and hepatocellular carcinogenesis in liver of female flounder (Platichthys flesus (L.))

    NARCIS (Netherlands)

    Koehler, Angela; Alpermann, Tilmann; Lauritzen, Bjarne; van Noorden, Cornelis J. F.

    2004-01-01

    Juvenile and adult female flounder (Platichthys flesus (L.)) were caught either in the estuary of the most polluted European river, the Elbe, or as controls in a reference site to study pollution-induced xenobiotic resistance in their livers in relation to pathological alterations. In juvenile fish,

  13. Characterizing and predicting the distribution of Baltic Sea flounder (Platichthys flesus) during the spawning season

    DEFF Research Database (Denmark)

    Orio, Alessandro; Bergström, Ulf; Casini, Michele

    2017-01-01

    Identification of essential fish habitats (EFH), such as spawning habitats, is important for nature conservation, sustainable fisheries management and marine spatial planning. Two sympatric flounder (Platichthys flesus) ecotypes are present in the Baltic Sea, pelagic and demersal spawning flounder...

  14. Tissue specific haemoglobin gene expression suggests adaptation to local marine conditions in North Sea flounder (Platichthys flesus L.)

    DEFF Research Database (Denmark)

    Larsen, P.F.; Eg Nielsen, Einar; Hansen, M.M.

    2013-01-01

    of the haemoglobin alpha and beta subunit genes was studied in reciprocally transplanted European flounder Platichthys flesus from the highly saline North Sea and the brackish Baltic Sea. Clear differences in expression patterns of haemoglobin alpha and beta subunit genes were found among different types of tissue....... Finally, for kidney tissue a stress response was observed in one population, with gene up-regulation when North Sea flounders were transplanted to low salinity. This study underlines the importance of tissue specific gene expression and the significance of gene expression for evolution of local adaptation...... in high gene flow marine fishes. © 2013 The Genetics Society of Korea...

  15. Molecular and biochemical studies on the Ah receptor pathway in flounder (Platichthys flesus)

    NARCIS (Netherlands)

    Besselink, H.

    1998-01-01

    The research presented in this thesis focused on the mechanistic aspects of the toxic and biochemical effects of PCBs in flounder (Platichthys flesus), with the aim to provide a scientific basis for the suggested involvement of PCBs in the aetiology of diseases observed

  16. Variation patterns in individual fish responses to chemical stress among estuaries, seasons and genders: the case of the European flounder (Platichthys flesus) in the Bay of Biscay.

    Science.gov (United States)

    Laroche, Jean; Gauthier, Olivier; Quiniou, Louis; Devaux, Alain; Bony, Sylvie; Evrard, Estérine; Cachot, Jérôme; Chérel, Yan; Larcher, Thibaut; Riso, Ricardo; Pichereau, Vianney; Devier, Marie Hélène; Budzinski, Hélène

    2013-02-01

    The objective was to describe and model variation patterns in individual fish responses to contaminants among estuaries, season and gender. Two hundred twenty-seven adult European flounders were collected in two seasons (winter and summer) in four estuaries along the Bay of Biscay (South West France), focusing on a pristine system (the Ster), vs. three estuaries displaying contrasted levels of contaminants (the Vilaine, Loire and Gironde). Twenty-three variables were measured by fish, considering the load of contaminants (liver metals, liver and muscle persistent organic pollutants, muscle polycyclic aromatic hydrocarbons); the gene expression (Cyt C oxydase, ATPase, BHMT, Cyt P450 1A1, ferritin); the blood genotoxicity (Comet test); and liver histology (foci of cellular alteration-tumour, steatosis, inflammation, abnormal glycogen storage). Canonical redundancy analysis (RDA) was used to model these variables using gender, season and estuary of origin as explanatory variables. The results underlined the homogeneity of fish responses within the pristine site (Ster) and more important seasonal variability within the three contaminated systems. The complete model RDA was significant and explained 35 % of total variance. Estuary and season respectively explained 30 and 5 % of the total independent variation components, whilst gender was not a significant factor. The first axis of the RDA explains nearly 27 % of the total variance and mostly represents a gradient of contamination. The links between the load of contaminants, the expression of several genes and the biomarkers were analysed considering different levels of chemical stress and a possible multi-stress, particularly in the Vilaine estuary.

  17. Laboratory maintenance, topographical anatomy and histology of flounder, Platichthys flesus

    NARCIS (Netherlands)

    Grinwis GCM; Wester PW; Kamstra A; van den Brandhof EJ; van Dijk JE; Leewis RJ; Vaal MA; Vethaak AD; Vos JG; PAT; UU/VF; RIVO/DLO; ECO; LWD; RIKZ

    1995-01-01

    Diverse veldonderzoeken suggereren een relatie tussen watervervuiling en enkele ziekten (b.v. huidzweren, vin-rot, lymphocystis infectie en levertumoren) bij vissen. Onderzoeken met de bot (Platichthys flesus), gehouden onder semi-veld (mesocosmos) condities, wijzen op een mogelijk causaal verband

  18. Characterizing and predicting the distribution of Baltic Sea flounder (Platichthys flesus) during the spawning season

    Science.gov (United States)

    Orio, Alessandro; Bergström, Ulf; Casini, Michele; Erlandsson, Mårten; Eschbaum, Redik; Hüssy, Karin; Lehmann, Andreas; Ložys, Linas; Ustups, Didzis; Florin, Ann-Britt

    2017-08-01

    Identification of essential fish habitats (EFH), such as spawning habitats, is important for nature conservation, sustainable fisheries management and marine spatial planning. Two sympatric flounder (Platichthys flesus) ecotypes are present in the Baltic Sea, pelagic and demersal spawning flounder, both displaying ecological and physiological adaptations to the low-salinity environment of this young inland sea. In this study we have addressed three main research questions: 1) What environmental conditions characterize the spatial distribution and abundance of adult flounder during the spawning season? 2) What are the main factors defining the habitats of the two flounder ecotypes during the spawning season? 3) Where are the potential spawning areas of flounder? We modelled catch per unit of effort (CPUE) of flounder from gillnet surveys conducted over the southern and central Baltic Sea in the spring of 2014 and 2015 using generalized additive models. A general model included all the stations fished during the survey while two other models, one for the demersal and one for the pelagic spawning flounder, included only the stations where each flounder ecotype should dominate. The general model captured distinct ecotype-specific signals as it identified dual salinity and water depth responses. The model for the demersal spawning flounder revealed a negative relation with the abundance of round goby (Neogobius melanostomus) and a positive relation with Secchi depth and cod abundance. Vegetation and substrate did not play an important role in the choice of habitat for the demersal ecotype. The model for the pelagic spawning flounder showed a negative relation with temperature and bottom current and a positive relation with salinity. Spatial predictions of potential spawning areas of flounder showed a decrease in habitat availability for the pelagic spawning flounder over the last 20 years in the central part of the Baltic Sea, which may explain part of the observed

  19. Wild and hatchery populations of Korean starry flounder (Platichthys stellatus) compared using microsatellite DNA markers.

    Science.gov (United States)

    An, Hye Suck; Byun, Soon Gyu; Kim, Yi Cheong; Lee, Jang Wook; Myeong, Jeong-In

    2011-01-01

    Starry flounder (Platichthys stellatus) is an important sport and food fish found around the margins of the North Pacific. Aquaculture production of this species in Korea has increased because of its commercial value. Microsatellite DNA markers are a useful DNA-based tool for monitoring the genetic variation of starry flounder populations. In this study, 12 polymorphic microsatellite DNA markers were identified from a partial genomic starry flounder DNA library enriched in CA repeats, and used to compare allelic variation between wild and hatchery starry flounder populations in Korea. All loci were readily amplified and demonstrated high allelic diversity, with the number of alleles ranging from 6 to 18 in the wild population and from 2 to 12 in the farmed population. A total of 136 alleles were detected at the 12 microsatellite loci in the two populations. The mean observed and expected heterozygosities were 0.62 and 0.68, respectively, in the hatchery samples and 0.67 and 0.75, respectively, in the wild samples. These results indicate lower genetic variability in the hatchery population as compared to the wild population. Significant shifts in allelic frequencies were detected at eight loci, which resulted in a small but significant genetic differences between the wild and hatchery populations (F(ST) = 0.043, P hatchery populations. These microsatellite loci may be valuable for future population genetic studies, monitoring the genetic variation for successful aquaculture management and the preservation of aquatic biodiversity.

  20. Liver histopathology in Baltic flounder (Platichthys flesus) as indicator of biological effects of contaminants.

    Science.gov (United States)

    Lang, Thomas; Wosniok, Werner; Barsiene, Janina; Broeg, Katja; Kopecka, Justyna; Parkkonen, Jari

    2006-01-01

    Results are presented of a study on liver histopathology in Baltic flounder (Platichthys flesus) carried out in 2001 and 2002 in four coastal sampling areas of the Baltic Sea: Kvädöfjärden (Swedish east coast, reference area), Klaipeda-Butinge (Lithuanian coast), Gulf of Gdansk (Polish coast), and Wismar Bay (German coast) within the framework of the EU-funded BEEP project. Liver lesions were diagnosed and categorised using standardised methodologies and, for a spatial and temporal assessment of the prevalence and types of lesions detected, a scoring system was applied, involving the calculation of mean histopathology lesion scores. 83.0% of the 436 female flounder examined (size range: 20-43 cm total length, age range: 2-8 years) were affected by liver lesions, out of which 74.3% were assigned to the category of non-specific, 3.4% to the category of early toxicopathic non-neoplastic, 4.6% to the category of pre-neoplastic and 0.7% to the category of neoplastic lesions. Mean lesions scores were highest in the areas at the Lithuanian and Swedish coast and there is indication of an impact of the age structure of the flounder populations studied, the sampling season as well as of contaminant effects.

  1. Early growth and development of reciprocal hybrids of the starry flounder Platichthys stellatus and stone flounder Kareius bicoloratus.

    Science.gov (United States)

    Yamashita, Y T; Aritaki, M; Kurita, Y; Tanaka, M

    2014-05-01

    Larval growth and development of hybrid flounder were observed and compared with those of their parent species. The reciprocal hybrids of female starry flounder Platichthys stellatus and male stone flounder Kareius bicoloratus (hybrid Sb) and of female K. bicoloratus and male P. stellatus (hybrid Bs) both survived and grew to juveniles. Development was divided into nine stages (A-I). Many of the hybrids' traits were identical and intermediate to those of their parents. The position of the eye, however, was primarily sinistral in both hybrids (80% in Sb and 76% in Bs), a trait possessed by P. stellatus (80%) in the western Pacific Ocean. The daily growth rates of the larvae were similar. In the parent species, development was more rapid in P. stellatus than in K. bicoloratus whereas rate of development was intermediate in both Sb and Bs hybrids. The size at settlement [standard length (LS ) at stage H (mean ± s.d.)] was 9·82 ± 1·47 mm for the hybrid Sb and 9·99 ± 0·90 mm for the hybrid Bs, while the minimum age at metamorphosis (initial age at stage H) was 29 days after hatching (DAH) in both hybrids. In comparison, LS at settlement in parent species was 6·43 ± 0·25 mm for P. stellatus and 12·87 ± 1·29 mm for K. bicoloratus. Minimum age at metamorphosis for the parents was 23 DAH at stage G in P. stellatus and 34 DAH at stage H in K. bicoloratus. Thus, the timing of settlement of hybrids was different from that of their parent species. These traits may occur with high frequency in a natural habitat. © 2014 The Fisheries Society of the British Isles.

  2. Clonal xenobiotic resistance during pollution-induced toxic injury and hepatocellular carcinogenesis in liver of female flounder (Platichthys flesus (L.)).

    Science.gov (United States)

    Koehler, Angela; Alpermann, Tilmann; Lauritzen, Bjarne; Van Noorden, Cornelis J F

    2004-01-01

    Juvenile and adult female flounder (Platichthys flesus (L.)) were caught either in the estuary of the most polluted European river, the Elbe, or as controls in a reference site to study pollution-induced xenobiotic resistance in their livers in relation to pathological alterations. In juvenile fish, livers displayed reversible and irreversible degenerative toxipathic lesion types but never showed (pre)neoplastic changes. Tumour frequencies up to 70% were found macroscopically in livers of adult female flounder which had progressed to adenomas and carcinomas in the most polluted site. Because male adult flounder show only up to 50% of livers containing early preneoplastic foci but never malignancies, we focussed our study on female individuals. (Pre)neoplastic changes ranged from early eosinophilic foci to basophilic foci, adenomas and hepatocellular carcinomas. Adenomas were generally eosinophilic whereas carcinomas were mainly basophilic. These phenotypical sequential changes strongly resemble those found in chemically-induced liver carcinogenesis in mammals. Characteristic mutations known from mammalian cancers have not been found so far in these flounder livers. Therefore, we investigated whether epigenetic events had induced a metabolic "resistant phenotype" of (pre)malignant cancer cells during hepatocellular carcinogenesis. With a quantitative immunohistochemical approach, we studied expression of P-glycoprotein (P-gp)-mediated multixenobiotic resistance (MXR), cytochrome P4501A1, glutathione-S-transferase-A which are key proteins in xenobiotic metabolism and elimination. Glucose-6-phosphate dehydrogenase (G6PDH) activity, the major source of the reducing power NADPH which is needed for biotransformation, oxyradical scavenging and biosynthesis, was detected as well. We observed upregulation of G6PDH activity already in early preneoplastic eosinophilic foci and subsequent further upregulation in basophilic foci and carcinomas. P-gp started to become

  3. Different organochlorine contaminant profiles in groups of flounders (Platichthys flesus) from sampling locations around Denmark

    DEFF Research Database (Denmark)

    Vorkamp, Katrin; Svendsen, Tore Christian; Rønsholdt, Bent

    2012-01-01

    Flounders (Platichthys flesus) from the waters around Denmark were analysed for their organochlorine (OC) profile to study whether fish from the same genetic population could be separated into characteristic subpopulations, based on their feeding grounds. The chemical analysis of fish liver...... provided a data set of 16 OC compounds in 94 samples from 2004 to 2006. Except for hexachlorocyclohexane, OC compounds were intercorrelated, indicating similar environmental fate and bioaccumulation. OC profiles are less affected than absolute concentrations by potentially confounding biological factors...... and thus more suitable for studies of intrapopulation differences in relation to feeding grounds. Principal component analysis grouped the samples according to locations. All but three of the 94 samples could be reclassified. Samples from the same and additional locations collected in 2003 provided...

  4. Ontogenetic changes in digestive enzyme activities and the amino acid profile of starry flounder Platichthys stellatus

    Science.gov (United States)

    Song, Zhidong; Wang, Jiying; Qiao, Hongjin; Li, Peiyu; Zhang, Limin; Xia, Bin

    2016-09-01

    Ontogenetic changes in digestive enzyme activities and the amino acid (AA) profile of starry flounder, Platichthys stellatus, were investigated and limiting amino acids were estimated compared with the essential AA profile between larvae and live food to clarify starry flounder larval nutritional requirements. Larvae were collected at the egg stage and 0, 2, 4, 7, 12, 17, 24 days after hatching (DAH) for analysis. Larvae grew from 1.91 mm at hatching to 12.13 mm at 24 DAH. Trypsin and chymotrypsin activities changed slightly by 4 DAH and then increased significantly 4 DAH. Pepsin activity increased sharply beginning 17 DAH. Lipase activity increased significantly 4 DAH and increased progressively with larval growth. Amylase activity was also detected in newly hatched larvae and increased 7 DAH followed by a gradual decrease. High free amino acid (FAA) content was detected in starry flounder eggs (110.72 mg/g dry weight). Total FAA content dropped to 43.29 mg/g in 4-DAH larvae and then decreased gradually to 13.74 mg/g in 24-DAH larvae. Most FAAs (except lysine and methionine) decreased >50% in 4-DAH larvae compared with those in eggs and then decreased to the lowest values in 24-DAH larvae. Changes in the protein amino acid (PAA) profile were much milder than those observed for FAAs. Most PAAs increased gradually during larval development, except lysine and phenylalanine. The percentages of free threonine, valine, isoleucine, and leucine decreased until the end of the trial, whereas the protein forms of these four AAs followed the opposite trend. A comparison of the essential AA composition of live food (rotifers, Artemia nauplii, and Artemia metanauplii) and larvae suggested that methionine was potentially the first limiting AA. These results may help develop starry flounder larviculture methods by solving the AA imbalance in live food. Moreover, the increased digestive enzyme activities indicate the possibility of introducing artificial compound feed.

  5. Health effects of some aquatic pollutants in European flounder : Laboratory experiments with emphasis on histopathological and immunological aspects

    NARCIS (Netherlands)

    Grinwis, G.C.M.

    2006-01-01

    Triggered by the concern over relative high prevalences of liver tumors, skin ulcers and the viral lymphocystis disease in European flounder (Platichthys flesus) living in Dutch coastal and estuarine waters, an integrated study was initiated to investigate a possible causal relationship between

  6. Comparison of common persistent organic pollutants (POPs) in flounder (Platichthys flesus) from the Vistula (Poland) and Douro (Portugal) River estuaries.

    Science.gov (United States)

    Waszak, Ilona; Dabrowska, Henryka; Komar-Szymczak, Katarzyna

    2014-04-15

    Groups of flounder (Platichthys flesus) females were collected in 2011 from the Vistula River and the Duoro River estuaries and corresponding reference sites in the southern Baltic Sea and Portuguese coast of the Atlantic Ocean to measure and compare the levels and profiles of polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), and polybrominated diphenyl ethers (PBDEs). The estuaries' sediments were also investigated. Several differences were found in the POPs between the estuaries and between the two marine regions, which were highlighted by PCA. The Vistula River estuary POPs, significantly higher than in the Douro River estuary, were dominated by DDTs followed by PCBs. PBDEs levels, indifferent between the estuaries, were relatively low. The POP levels in flounder and sediment evaluated against environmental assessment criteria (EACs) indicated that none of the measured contaminants for which EAC had been established exceeded the criterion, except for CB-118 in flounder from the Vistula River estuary. Copyright © 2014 Elsevier Ltd. All rights reserved.

  7. Spatial distribution of grossly visible diseases and parasites in flounder ( Platichthys flesus ) from the Baltic Sea : a synoptic survey

    DEFF Research Database (Denmark)

    Lang, T.; Mellergaard, Stig; Wosniok, W.

    1999-01-01

    of a multivariate statistical analysis reveal that the diseases are influenced by a variety of host-specific (length, age, sex) and area-specific (salinity, temperature) factors as well as their interactions. By calculating the expected prevalence for a standardized fish population for each area and disease......Information on prevalences of grossly visible diseases and parasites of flounder (Platichthys flesus) from the Baltic Sea is presented for 11 sampling areas on a transect from the Mecklenburg Eight to the Gulf of Finland. Among the 3008 flounder examined, highest overall prevalences were observed...... for lymphocystis (14.4%) and acute/healing stages of the skin ulcer disease (5.9%). The prevalences of liver neoplasms >2 mm in diameter (0.4%), skeletal deformities (0.6%), and fin rot/erosion (0.5%) were low. The only externally visible parasite recorded was Cryptocotyle concavum (28.2%). The results...

  8. Biomarker responses in flounder (Platichthys flesus) and mussel (Mytilus edulis) in the Klaipeda-Būtinge area (Baltic Sea).

    Science.gov (United States)

    Barsiene, Janina; Lehtonen, Kari K; Koehler, Angela; Broeg, Katja; Vuorinen, Pekka J; Lang, Thomas; Pempkowiak, Janusz; Syvokiene, Janina; Dedonyte, Veronika; Rybakovas, Aleksandras; Repecka, Rimantas; Vuontisjärvi, Heta; Kopecka, Justyna

    2006-01-01

    During the EU project BEEP a battery of biomarkers was applied in flounder (Platichthys flesus) and the blue mussel (Mytilus edulis) collected at three locations off the Lithuanian coast (Baltic Sea) in June and September 2001 and 2002. The elevated biomarker responses in specimens sampled in September 2001 were apparently related to the extensive dredging activities in the Klaipeda port area and subsequent dumping of contaminated sediments. High concentrations of organic pollutants (organochlorines and PBDEs) were also measured in the tissues of both indicator species. In addition, response levels of genotoxicity, cytotoxicity, immunotoxicity as well as concentrations of PAH metabolites in the bile of flounder showed elevations in 2002 after an oil spill in the Būtinge oil terminal in November 2001. In flounder, biomarker measurements 10 months after the spill indicated recovery processes but in mussels a high level of genotoxicity could still be observed 22 months later. The present study illustrates the usefulness of the multi-biomarker approach in the detection of biological effects of pollution in this region of the Baltic Sea.

  9. Histopathological examinations of liver nodules in flounder ( Platichthys flesus L.) from the Baltic Sea

    DEFF Research Database (Denmark)

    Bogovski, S.; Lang, T.; Mellergaard, Stig

    1999-01-01

    Grossly visible hepatic nodular lesions in 34 flounder collected during the BMB/ICES Sea-going Workshop "Fish Diseases and Parasites in the Baltic Sea", 25 November to 8 December, 1994, are described histopathologically. Foci of cellular alteration were recorded only in three fish from the southw......Grossly visible hepatic nodular lesions in 34 flounder collected during the BMB/ICES Sea-going Workshop "Fish Diseases and Parasites in the Baltic Sea", 25 November to 8 December, 1994, are described histopathologically. Foci of cellular alteration were recorded only in three fish from...

  10. Evidences for involvement of growth hormone and insulin-like growth factor in ovarian development of starry flounder (Platichthys stellatus).

    Science.gov (United States)

    Xu, Yongjiang; Wang, Bin; Liu, Xuezhou; Shi, Bao; Zang, Kun

    2017-04-01

    Although gonadotrophins are major regulators of ovarian function in teleosts and other vertebrates, accumulating evidence indicates that the growth hormone (GH)-insulin-like growth factor (IGF) axis also plays an important role in fish reproduction. As a first step to understand the physiological role of the GH-IGF system in the ovarian development of starry flounder (Platichthys stellatus), the expression profiles of GH and IGF messenger RNAs (mRNAs) and plasma GH, IGF-I, estradiol-17β (E2), and testosterone (T) levels during the ovarian development were investigated. The developmental stages of ovaries were divided into five stages (II, III, IV, V, and VI) by histological analysis. The hepatosomatic index (HSI) and gonadosomatic index (GSI) values increased and peaked at stage IV and stage V, respectively, and then declined at stage VI. Pituitary GH mRNA levels decreased sharply at stage III and raised to top level at stage VI. The hepatic IGF-I mRNA levels ascended to maximum value at stage V and then declined significantly at stage VI. However, the hepatic IGF-II mRNA levels remained stable and increased significantly at stage VI. In contrast, the ovarian IGF-I mRNA levels increased gradually and peaked at stage VI. The ovarian IGF-II mRNA levels were initially stable and increased significantly at stage V until the top level at stage VI. Consistent with the pituitary GH mRNA levels, plasma GH levels reduced sharply at stage III and remained depressed until stage V and then raised remarkably at stage VI. Plasma IGF-I level peaked at stage V and then declined to initial level. Plasma E2 level peaked at stage IV and then dramatically descended to the basal level. Plasma T level peaked at stage V and then declined significantly back to the basal level. Based on statistical analysis, significant positive correlations between hepatic IGF-I mRNA and GSI, ovarian IGF-II mRNA and hepatic IGF-II mRNA, ovarian IGF-I mRNA and ovarian IGF-II mRNA, and plasma IGF-I and

  11. Parasites of flounder Platichthys flesus (L.) from the German Bight, North Sea, and their potential use in ecosystem monitoring. B. Community structure and fish parasite biodiversity

    Science.gov (United States)

    Schmidt, V.; Zander, S.; Körting, W.; Steinhagen, D.

    2003-10-01

    The analysis of fish-parasite community structure and the use of ecological richness and diversity measurements are commonly used for the evaluation of environmental stress in aquatic ecosystems. As part of an integrated biological-effect monitoring, the parasite community of flounder Platichthys flesus (L.) was investigated for various locations in the German Bight during spring and autumn of 1995-2000, using established ecological methods. Although the parasite-community composition was very similar at the component-community level, the number of component species as well as the species accumulation curves showed clear differences among the sites. On the infra-community level, all of the ecological measurements showed significantly lower values in flounder from the Elbe estuary, the most polluted site, than in flounder from Helgoland. This was seen during a single season or during both seasons. When the data were pooled over the years, gradual differences between the sites, which were seldom detected at individual sampling periods, became evident for different measurements of species richness and species diversity and corresponded to a contamination gradient established between Elbe > Inner Eider, Outer Eider > Helgoland. Despite seasonal variations, which were observed in almost all measurements, these gradual differences were found in both seasons.

  12. Survey of estrogenic activity in United Kingdom estuarine and coastal waters and its effects on gonadal development of the flounder Platichthys flesus

    Energy Technology Data Exchange (ETDEWEB)

    Allen, Y.; Scott, A.P.; Matthiessen, P.; Haworth, S.; Thain, J.E.; Feist, S.

    1999-08-01

    Estrogenic discharges are known or suspected to be present in many United Kingdom estuaries and are also occurring from offshore oil and gas installations. The aim of this study was to establish whether estrogens and their mimics are present in marine waters at concentrations that can produce biological responses in fish and, if so, to discover whether the effects are likely to be harmful to populations and communities through changes in reproductive and other physiological processes. Laboratory studies and extensive field surveys were carried out with the euryhaline flounder (Platichthys flesus), using the amount of yolk protein vitellogenin in the blood of free-living male fish as an indicator of exposure to estrogens. Exposure to the synthetic estrogen ethynylestradiol for 3 weeks confirmed that the vitellogenin response was made and showed that this species is about one order of magnitude less sensitive than the freshwater species rainbow trout. The fish did not respond to the estrogen mimic nonylphenol at concentrations of 30 {micro}g/L. Wild male flounder were sampled from four contaminated United Kingdom estuaries: the Tyne, Crouch, Thames, and Mersey. Fish from one or more sites in each estuary had significantly elevated concentrations of vitellogenin in their blood when compared to concentrations in fish from the clean control estuary (Alde River). Vitellogenin concentrations in the Tyne and Mersey fish were four and six orders of magnitude greater, respectively, than control concentrations. Elevated vitellogenin concentrations were also seen in some male flounder caught in coastal areas. Approximately 20% of male fish from the Mersey estuary contained oocytes in their testes, but this intersex condition was not seen elsewhere. The implications of these findings for fish populations are discussed.

  13. Comparison of the effects of dietary single and multi-probiotics on growth, non-specific immune responses and disease resistance in starry flounder, Platichthys stellatus.

    Science.gov (United States)

    Park, Youngjin; Moniruzzaman, Mohammad; Lee, Seunghan; Hong, Jeongwhui; Won, Seonghun; Lee, Jong Min; Yun, Hyeonho; Kim, Kang-Woong; Ko, Daegyun; Bai, Sungchul C

    2016-12-01

    An 8-week feeding trial was conducted to evaluate the effects of dietary probiotics on growth performance and non-specific immune responses in starry flounder, Platichthys stellatus. Fish averaging 46.5 ± 0.65 g (mean ± SD) were fed one of the six experimental diets; one control (Cont), and five other diets were prepared by supplementing single-probiotics 1 (Bacillus subtilis; SP1, 2 × 109 CFU kg-1 diet), single-probiotics 2 (Bacillus licheniformis; SP2, 2 × 109 CFU kg-1 diet), multi-probiotics 1 (Bacillus subtilis + Bacillus licheniformis; MP1, 2 × 109 CFU kg-1 diet), multi-probiotics 2 (commercial probiotics; Bacillus subtills + Bacillus licheniformis + Paenibacillus polymyxa + Aspergillus oryzae + Saccharomyces cerevisiae; MP2, 2 × 109 CFU kg-1 diet) and oxytetracycline (OTC) at 5 g OTC kg-1 diet. At the end of 8 weeks feeding trial, weight gain (WG) and specific growth rate (SGR) of fish fed SP1, MP1 and MP2 diets were significantly higher than those of fish fed control diet (P probiotics were equally effective statistically. These results demonstrated that single or multi-probiotics had equal beneficial effect as an antibiotic replacer in terms of growth performance, non-specific immune responses and disease resistance in starry flounder. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. Distribution and elimination of (/sup 14/C)-hexachlorobenzene after single oral exposure in cod (Gadus morhua) and flounder (Platichthys flesus)

    Energy Technology Data Exchange (ETDEWEB)

    Ingebrigtsen, K.; Solbakken, J.E.

    1985-01-01

    Distribution and elimination of hexachlorobenzene (HCB) after administration to COD (Gadus morhua) of a single oral dose of 5 microCi (/sup 14/C)HCB/100 g body weight were studied by whole-body autoradiography and liquid scintillation counting. To obtain some information on the physicochemical properties of the radiolabeled compounds, whole-body autoradiography was performed exposing parallel sagittal sections, treated at -20/sup 0/C, evaporated at 50/sup 0/C, and extracted separately with polar and nonpolar solvents. The highest concentration of radioactivity was found in the liver, the bile, and the central nervous system (CNS). Radioactivity in the liver and CNS, which was completely evaporable, was considered to represent the highly volatile HCB itself, and/or metabolites with high vapor pressure. No part of radioactivity in bile was evaporable, but it was completely extractable with water. Radioactivity in the intestinal content, the skin, and the uveal tract of the eye was partly evaporable. No radioactivity remained in any tissue after extraction with polar and nonpolar solvents. The rate of elimination was slow, and substantial amounts remained in the body 60 d after administration. In addition to bile excretion of nonevaporable, water-soluble radioactivity, a possible excretion through the intestinal mucosa was suggested. Whole-body autoradiography of female flounders (Platichthys flesus) revealed a high content of radioactivity in the developing eggs.

  15. Insulin-like growth factors I and II in starry flounder (Platichthys stellatus): molecular cloning and differential expression during embryonic development.

    Science.gov (United States)

    Xu, Yongjiang; Zang, Kun; Liu, Xuezhou; Shi, Bao; Li, Cunyu; Shi, Xueying

    2015-02-01

    In order to elucidate the possible roles of insulin-like growth factors I and II (IGF-I and IGF-II) in the embryonic development of Platichthys stellatus, their cDNAs were isolated and their spatial expression pattern in adult organs and temporal expression pattern throughout embryonic development were examined by quantitative real-time PCR assay. The IGF-I cDNA sequence was 1,268 bp in length and contained an open reading frame (ORF) of 558 bp, which encoded 185 amino acid residues. With respect to IGF-II, the full-length cDNA was 899 bp in length and contained a 648-bp ORF, which encoded 215 amino acid residues. The amino acid sequences of IGF-I and IGF-II exhibited high identities with their fish counterparts. The highest IGF-I mRNA level was found in the liver for both sexes, whereas the IGF-II gene was most abundantly expressed in female liver and male liver, gill, and brain. The sex-specific and spatial expression patterns of IGF-I and IGF-II mRNAs are thought to be related to the sexually dimorphic growth and development of starry flounder. Both IGF-I and IGF-II mRNAs were detected in unfertilized eggs, which indicated that IGF-I and IGF-II were parentally transmitted. Nineteen embryonic development stages were tested. IGF-I mRNA level remained high from unfertilized eggs to low blastula followed by a significant decrease at early gastrula and then maintained a lower level. In contrast, IGF-II mRNA level was low from unfertilized eggs to high blastula and peaked at low blastula followed by a gradual decrease. Moreover, higher levels of IGF-I mRNA than that of IGF-II were found from unfertilized eggs to high blastula, vice versa from low blastula to newly hatched larva, and the different expression pattern verified the differential roles of IGF-I and IGF-II in starry flounder embryonic development. These results could help in understanding the endocrine mechanism involved in the early development and growth of starry flounder.

  16. Liver histopathology of flounder (Platichthys flesus) in Dutch coastal and estuarine waters, with particular reference to neoplastic disease and their relationship with environmental factors

    NARCIS (Netherlands)

    Vethaak AD; Wester PW; RIKZ; PAT

    1994-01-01

    Dit rapport beschrijft het histopathologisch onderzoek van levers van platvis (bot, Platichthys flesus) afkomstig van de Nederlandse kustwateren en estuaria welke bemonsterd zijn in de periode 1985-89. Bemonstering vond plaats op een 10-tal lokaties waarbij 210 levers zijn verzameld met

  17. Relationship between biomarker responses and contaminant concentration in selected tissues of flounder (Platichthys flesus from the Polish coastal area of the Baltic Sea

    Directory of Open Access Journals (Sweden)

    Magdalena Podolska

    2008-09-01

    Full Text Available Previous studies in the Gulf of Gdańsk discussed the responses of selected enzymatic biomarkers to the contaminant gradient in fish and mussels. In the present study, flounder muscle and liver tissues were analyzed for polychlorinated biphenyls (PCB congeners: 28, 52, 101, 118, 138, 153 and 180, organochlorine pesticides (HCHs, HCB and DDTs, and trace metals (Pb, Cd, Zn, Cu, Hg, Cr. An attempt was made to identify the relationship between the measured enzymatic biomarker responses (cholinesterases, malic enzyme, isocitrate dehydrogenase, glutathione S-transferase and contaminant concentrations in selected flounder tissues. The observed differences in enzymatic biomarker levels suggest that chronic exposure to low-concentration mixtures of contaminants may be occurring in the studied area. However, no conclusive evidence was found of a clear link between the biomarker responses and contaminant concentrations in flounder tissues.

  18. Density, growth and annual food consumption of plaice (Pleuronectes platessa L.) and flounder (Platichthys flesus (L.)) in Lake Grevelingen, The Netherlands

    Science.gov (United States)

    Doornbos, G.; Twisk, F.

    Within the scope of a carbon budget study in the 108 km 2 saline Lake Grevelingen, investigations on the plaice and flounder populations were made during October 1979 to December 1981. Both populations were dominated by the 1979 year class that presumably entered the lake as larvae in the year the Brouwerssluice, which opens to the North Sea, was operative the whole year round. Due to high summer temperatures in the lake, juvenile plaice (year class 1981) attained a mean length of 13.0 cm and a fresh weight of 27.4 g at the end of their first growing season. At the end of their third year, year class 1979 males measured on average 27.7 cm and females 29.7 cm, which is about 3.5 cm more than plaice of the same age in the North Sea. Flounder reached a mean length of 26.3 cm in 3 years. Total stock of plaice, excluding year class 1981, was estimated at an average of 1.3 million over 1980 and at 0.6 million fish over 1981. For flounder these figures were 0.7 million and 0.2 million and 0.2 million fish, respectively. In September 1981 the number of O-group plaice was assessed at about 0.2 million individuals. About 56% of the plaice food consisted of polychaetes, mainly consisting of in 1980 Arenicola marina (41%) and in 1981 A. marina (15%), Nereidae (15%) and Nephtys hombergii (11%). Larger plaice consumed significantly more Arenicola and less Tharyx marioni than smaller ones. Polychaetes and crustaceans were eaten the whole year rounf while the consumption of molluscs was restricted to the summer period and the of fisg to autumn and winter. In 1980 the flounder population preyed predominantly upon crustaceans (41%) and polychaetes (37%), with Arenicola marina (36%) and Crangon crangon (25%) as the most important species. In 1981 relatively more polychaetes (53%) and less crustaceans (14%) were eaten. Total consumption of plaice population, excluding year class 1981, in 1980 and 1981 was estimated at 1.68 g and 1.41 g ADW·m -2·a -1. Annual consumption of O

  19. Variability of biological indices, biomarkers, and organochlorine contaminants in flounder (Platichthys flesus) in the Gulf of Gdańsk, southern Baltic Sea.

    Science.gov (United States)

    Kopko, Orest; Dabrowska, Henryka

    2017-12-08

    Biological indices, biomarkers (EROD activity, DNA SB, 1-OH pyrene), and organochlorine contaminants were investigated in flounder collected in the Gulf of Gdańsk during March-December 2011 to describe their seasonal variability and interrelations. Univariate and multivariate statistics were used to evaluate the relations. The EROD activity positively correlated with DNA SB, both negatively correlated with CF, GSI, and HSI, and there was a moderate positive correlation for EROD and DNA SB with 1-OH pyrene. EROD highest activity corresponded to a resting stage of gonad development. DNA SB, highest during spawning, gradually decreased until late autumn. The PCBs, DDTs, HCB, HCHs, dieldrin, and heptachlor levels in muscle tissue were quantified on a wet and lipid basis. In each case, their levels decreased after spawning, fluctuated over the study period indicating that their accumulation was pronouncedly controlled by chemical-specific properties, their levels in prey, and lipid dynamics. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Toxic effects and depuration after the dietary lead(II) exposure on the bioaccumulation and hematological parameters in starry flounder (Platichthys stellatus).

    Science.gov (United States)

    Hwang, In-Ki; Kim, Kyeong-Wook; Kim, Jun-Hwan; Kang, Ju-Chan

    2016-07-01

    Platichthys stellatus (mean length 20±2cm, mean weight 160.15±15g) were exposed to the different levels of dietary lead(II) at the concentrations of 0, 30, 60, 120, 240mg/kg for 4 weeks. Depuration was conducted for 2 weeks after exposure. The lead exposure over 60mg Pb/kg induced the significant bioaccumulation in tissues of P. stellatus (5-30μg/g tissue), except for brain and muscle where the exposure to 240mg Pb/kg caused the bioaccumulation (2-4μg/g tissue). The hematological parameters such as red blood cell (RBC) counts, hematocrit (Ht) value and hemoglobin (Hb) concentration were substantially decreased over 60mg Pb/kg, and lasted even after the depuration period. For plasma components, calcium and magnesium levels in plasma were generally decreased over 60mg Pb/kg, and glucose level was also mainly increased over 60mg Pb/kg. Total protein was significantly decreased over 120mg Pb/kg after 4 weeks exposure. Glucose and total protein showed the restoration after the depuration period in groups of fish exposed previously to over 60 and 120mg Pb/kg, respectively. However, other parameters that changed during the exposure over 60mg Pb/kg did not recovered. For enzymatic components in plasma, glutamic oxalate transminase (GOT), glutamic pyruvate transminase (GPT) and alkaline phosphatase (ALP) were significantly increased over 120mg Pb/kg, and there was only restoration observed after the depuration for ALP over 120mg Pb/kg. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Evolutionary mechanisms shaping the genetic population structure of marine fishes; lessons from the European flounder ( Platichthys flesus L.)

    DEFF Research Database (Denmark)

    Hansen, Jakob Hemmer; Eg Nielsen, Einar; Grønkjær, P.

    2007-01-01

    A number of evolutionary mechanisms have been suggested for generating low but significant genetic structuring among marine fish populations. We used nine microsatellite loci and recently developed methods in landscape genetics and coalescence-based estimation of historical gene flow and effectiv...... interplay with other evolutionary mechanisms, highlighting the importance of investigating species with wide geographical and ecological distributions to increase our understanding of evolution in the marine environment.......A number of evolutionary mechanisms have been suggested for generating low but significant genetic structuring among marine fish populations. We used nine microsatellite loci and recently developed methods in landscape genetics and coalescence-based estimation of historical gene flow and effective...... and western Baltic Sea samples. Alternative factors, such as dispersal potential and/or environmental gradients, could be important for generating genetic divergence in this region. The results show that the magnitude and scale of structuring generated by a specific mechanism depend critically on its...

  2. Habitat selection of stone and starry flounders in an estuary in relation to feeding and survival

    Science.gov (United States)

    Tomiyama, Takeshi; Omori, Michio

    2008-09-01

    Juveniles of both stone flounder Platichthys bicoloratus and starry flounder Platichthys stellatus utilize estuaries as nursery grounds. To understand their habitat selection and the functions of habitats such as food supply, we defined the seasonal distribution of recently settled fish of these species in shallow nursery areas and investigated their feeding habits in the Natori River estuary, Japan. Distribution of stone flounder was limited to the lower estuary (olivacea and the mysid Neomysis awatschensis, respectively; however, 1- and 2-yr-old fish of both stone and starry flounders fed mainly on the bivalve siphons. These results indicate that habitat selections of juvenile stone and starry flounders enable utilization of preferred prey and predator avoidance, respectively, and that non-overlap of these species' habitats results in avoidance of inter-specific competition for food between these two species.

  3. Quantitative cytochemical analysis of glucose-6-phosphate dehydrogenase activity in living isolated hepatocytes of European flounder for rapid analysis of xenobiotic effects

    NARCIS (Netherlands)

    Winzer, K.; van Noorden, C. J.; Köhler, A.

    2001-01-01

    There is a great need for rapid but reliable assays to determine quantitatively effects of xenobiotics on biological systems in environmental research. Hepatocytes of European flounder are sensitive to low-dose toxic stress. Glucose-6-phosphate dehydrogenase (G6PDH) is the major source of NADPH in

  4. Intraspecific variation in expression of candidate genes for osmoregulation, heme biosynthesis and stress resistance suggests local adaptation in European flounder ( Platichthys flesus )

    DEFF Research Database (Denmark)

    Larsen, Peter Foged; Eg Nielsen, Einar; Williams, T.D.

    2008-01-01

    Despite the recent discovery of significant genetic structuring in a large number of marine organisms, the evolutionary significance of these often minute genetic differences are still poorly understood. To elucidate the adaptive relevance of low genetic differentiation among marine fish populati...

  5. High doses of corticosteroid suppress resistance to Ichthyophonus in starry flounder

    Science.gov (United States)

    Perry, J.A.; Kocan, R.M.; Winton, J.R.; Hershberger, P.K.

    2004-01-01

    Application of pharmacological doses of the corticosteroid dexamethasone phosphate to starry flounder Platichthys stellatus resulted in a predisposition to clinical ichthyophoniasis and a progression from latent Ichthyophonus infections to patent, histologically identifiable infections. Among Ichthyophonus-challenged starry flounder, the prevalences of clinical infections and histologically identifiable infections were significantly greater in two groups that received dexamethasone (100% and 31%, respectively) than in the respective control groups (8% and 0%). Proliferation of Ichthyophonus infections in corticosteroid-treated groups may have resulted from suppression of the cellular immune response that typically follows corticosteroid application; however, further studies are needed to determine whether these effects occur at lower, physiological concentrations of corticosteroids.

  6. Trophic interactions of Platichthys flesus and Solea solea juveniles in the Lima estuary nursery (NW Portugal

    Directory of Open Access Journals (Sweden)

    Cláudia Mendes

    2015-12-01

    Full Text Available Trophic interactions play a key role in nursery habitats, and by affecting growth and condition of the juveniles, may control the quality of a given estuarine nursery. This study investigated the trophic ecology of flounder (Platichthys flesus and common sole (Solea solea juveniles in the Lima estuary nursery. Feeding location, main organic matter sources, and prey of the target species were assessed by carbon (δ13C and nitrogen (δ15N stable isotopes, as well as by stomach content analysis. The juveniles, macroinvertebrates, and sediment and water column samples were collected in August 2014 in the lower, middle and upper sections of the Lima estuary. The diet of 0+ flounder relied upon prey from the upper estuary (salinity >5, namely Chironomid larvae and Corophium spp. which showed the role of the upper estuary prey to the 0+ flounder diet and suggest the relative site fidelity of the young juveniles. In contrast, 1+ flounder juveniles had a diverse diet based on bivalves, polychaetes and crustaceans, and a variable stable isotope signature indicating they fed in different areas along the estuary. The 1+ sole juveniles also fed on polychaetes, crustaceans, and bivalves, but the stable isotope values suggested a dependence on the lower estuary (salinity >30 and marine food web sources. Such differential use of food may be understood as a strategic approach to reduce intra- and interspecific competition and thus ensuring the use of Lima estuary as nursery area for these two flatfish species.

  7. Food intake of young cod and flounder in a shallow bay on the Swedish west coast

    Science.gov (United States)

    Pihl, L.

    The importance of shallow soft bottom areas as nursery grounds for I-ground cod, Gadus morhua, and I- to IV-group flounder, Platichthys flesus, was investigated in a 20 000 m 2 bay, 0 to 1 m deep, on the Swedish west coast. To estimate the amount of visiting fish and their food intake the bay was enclosed with a net once a month from July to November 1979 and from April to November 1980. At each occasion about 60 cods and 8 to 50 flounders were caught. The total biomass of the cod and flounder was 1 to 14 kg and 2 to 13 kg wet weight, respectively. The cod preyed on mobile epifauna such as Carcinus maenas, Crangon crangon and Pomatoschistus spp., whereas the flounder was eating infauna mainly Mya arenaria, Cardium spp. and Nereis spp. The food intake within the area was calculated to approximate 35 and 10 kg wet weight from April to November for cod and flounder, respectively.

  8. Distributions of pefloxacin in flounder

    Energy Technology Data Exchange (ETDEWEB)

    Lee, P.S.; Jang, W.C. [Dankook University, Chonan (Korea, Republic of)

    1999-02-01

    This study showed the distribution of pefloxacin as a kind of quinolones after oral administrations of 100 mg/kg pefloxacin for 2 days in flounder. After administrations of pefloxacin, blood, muscle, and 8 organs including liver of flounder were analyzed by HPLC. In results, we knew that pefloxacin had absorbed from intestine and accumulated into gall bladder. In blood, maximum plasma concentration was showed 4.70 {mu}g/ml at 45 min, and detected 0.31{+-}0.01 {mu}g/ml at the eleventh day after administration. The concentration of pefloxacin was remained 0.29{+-}0.02 {mu}g/g tissue in muscle at the tenth day. 17 refs., 6 figs., 4 tabs.

  9. Comparative genetic variability between broodstock and offspring populations of Korean starry flounder used for stock enhancement in a hatchery by using microsatellite DNA analyses.

    Science.gov (United States)

    An, H S; Shin, E-H; Lee, J W; Nam, M M; Myeong, J I; An, C M

    2013-12-04

    Korean starry flounder, Platichthys stellatus (Pleuronectidae), is one of the most economically important fishery resources in Korea. We investigated the effect of current artificial reproduction in a hatchery facility, genetic divergence between the broodstock and their offspring populations of starry flounder in a hatchery strain to be stocked into natural sea areas was accessed using 9 polymorphic nuclear microsatellite DNA loci. High levels of polymorphism were observed between the 2 populations. A total of 96 alleles were detected at the loci, with some alleles being unique in the broodstock. Allelic variability ranged from 8 to 17 in the broodstock and from 7 to 12 in the offspring population. Average observed and expected heterozygosities were estimated at 0.565 and 0.741 in the broodstock samples and 0.629 and 0.698 in the offspring population, respectively. Although no statistically significant reductions were found in heterozygosity or allelic diversity in the offspring population, a considerable loss of rare alleles was observed in the offspring population compared with that in the broodstock. Significant genetic difference was detected between the broodstock and offspring populations (FST = 0.021, P hatchery operation in order to improve the starry flounder hatchery management. This information might be useful for fishery management and aquaculture industry of P. stellatus.

  10. Can analysis of Platichthys flesus otoliths provide relevant data on historical metal pollution in estuaries? Experimental and in situ approaches

    Energy Technology Data Exchange (ETDEWEB)

    Selleslagh, Jonathan, E-mail: Jonathan.Selleslagh@u-bordeaux.fr [Université de Bordeaux, CNRS, UMR EPOC 5805, Station Marine d' Arcachon, place du docteur Peyneau, 33120 Arcachon (France); Echard, Aurélie [IRSTEA Centre de Bordeaux, unité EABX, 50 avenue de Verdun, 33612 Cestas (France); Pécheyran, Christophe [Université de Pau et des pays de l' Adour, CNRS, LCABIE-IPREM UMR 5254, 64053 Pau (France); Baudrimont, Magalie [Université de Bordeaux, CNRS, UMR EPOC 5805, Station Marine d' Arcachon, place du docteur Peyneau, 33120 Arcachon (France); Lobry, Jérémy; Daverat, Françoise [IRSTEA Centre de Bordeaux, unité EABX, 50 avenue de Verdun, 33612 Cestas (France)

    2016-07-01

    Despite recent efforts to manage them more efficiently, estuaries are natural sinks for a wide range of metal contaminants, many of which accumulate at potentially toxic concentrations for fish populations, posing a threat to recruitment and stocks. While analysis of metal concentrations in soft tissue and water samples calls for continuous and long-term sampling operations, the use of otoliths to study metal pollution may be one way of providing a historical record of pollutant exposure. In this study, we examine the potential use of otoliths as natural tracers of metal contamination. A “cocktail” of different metals (Cd, Pb and Ni) was used to test bio-accumulation in otoliths and tissue (liver, kidney, muscle and gills) extracted from juvenile flounder (Platichthys flesus). Assessment took place under controlled conditions over a three month period, with water exposure concentrations increasing every 3 weeks. The concentrations used were natural (T1), X5 (T2), X10 (T3), and null (T4). Chemical analyses were carried out using an inductively coupled plasma optical emission spectrometer ICP-OES and atomic absorption spectrometer AAS for water and tissue, while otolith microchemistry analyses were performed using a femtosecond laser ablation-high resolution inductively coupled plasma mass spectrometer (fsLA-ICP-MS-HR). Significant differences between control and exposed individuals, as well as an increase in metal concentrations according to exposure level, were observed in all tissues except in muscle tissue. Significant increases in Pb were also detected in contaminated fish otoliths compared with control specimens, with the highest concentrations occurring in T3. Cartographies of Pb distribution in otoliths of both control and contaminated fish only showed high concentrations of Pb at the edge of contaminated fish otoliths, indicating an accumulation of metal during the experiment. Although the relationships between exposure level and Pb concentration in

  11. Short term toxicity of bis(tri-n-butyltin)oxide in flounder(Platichthys flesus) ; pathology and immune function

    NARCIS (Netherlands)

    Grinwis GCM; Brandhof EJ van den; Dormans JAMA; Engelsma M; Kuiper R; Leewis R; Loveren H van; Wester PW; Vaal MA; Vethaak AD; Vos JG; LWD; LPI; ECO; UU; RIKZ

    1997-01-01

    Field studies in various polluted coastal areas in Europe and the United States of America clearly indicate a relation between pollution and the increase in prevalence of tumours and infectious diseases in fish. One of the chemicals of interest in the myriad of xenobiotics found in polluted waters

  12. The quality of release habitat for reared juvenile flounder, Platichthys fl esus, with respect to salinity and depth

    DEFF Research Database (Denmark)

    Andersen, A.K.; Schou, J.; Sparrevohn, Claus Reedtz

    2005-01-01

    movement was examined, using a diffusion model, at the first two sites. At HV the fish had unrestricted dispersal opportunity resulting in average individual movement of 45 m day(-1). Fish at V were restricted in dispersal in one direction because of a dam, about which they would concentrate under certain.......), was examined in Danish inshore waters using enclosures to study growth and survival. Three locations were chosen: Virksund (V) - constant low salinity at 10 parts per thousand; Harre Vig (HV) - constant high salinity at 24 parts per thousand; and Hjerk Nor - variable salinity, 0-25 parts per thousand. Fish...

  13. Growth of winter flounder (Pseudopleuronectes americanus) and smooth flounder (Liopsetta putnami) in heated and unheated water

    Energy Technology Data Exchange (ETDEWEB)

    Hoornbeek, F.K. (Univ. of New Hampshire, Durham); Sawyer, P.J.; Sawyer, E.S.

    1982-07-01

    O-group and I-group winter (Pseudopleuronetes americanus) and smooth (Liopsetta putnami) flounder were reared at the Jackson Estuarine Laboratory, Durham, NH, U.S.A., between November 1975 and June 1976. Both species gained weight approximately three times more rapidly in heated than in unheated water. In unheated water the smallest winter flounder gained, on average, 116% of their body weight per month. Larger O-group winter flounder increased body weight by 55% per month, while comparably sized female and male smooth flounder gained 52% and 28% per month, respectively. I-group female smooth flounder gained 9.5% and male smooth flounder 8.5% per month in heated water. In unheated water increases were 13%, 22%, and 14% per month for O-group winter, female smooth and male smooth flounder, respectively. I-group winter flounder in unheated water gained weight twice as rapidly (9.5%) per month) as I-group female and male smooth flounder (4.0% and 4.5% per month, respectively). Fish were fed a moist diet at a level of 10% of their body weight per day. Conversions (dry weight of food/wet weight of fish) ranged from 1:1 for O-group winter flounder in heated water to 27:1 for I-group smooth flounder in unheated water. Disease was a major cause of mortality. Vibrio anguillarum was confirmed as a pathogen; myxobacteria, and the protozoan parasites Kudoa sp. and Nosema sp. were associated with losses.

  14. Functional characterization of two melanin-concentrating hormone genes in the color camouflage, hypermelanosis, and appetite of starry flounder.

    Science.gov (United States)

    Kang, Duk-Young; Kim, Hyo-Chan

    2013-08-01

    To investigate the involvement of two melanin-concentrating hormones (MCHs) in skin color change and appetite in flatfish, we isolated two forms of prepro-melanin concentrating hormone (pMCHs) mRNA in the starry flounder Platichthys stellatus and compared their amino acid structures to those of other animals. Then, we examined the relationship of the two starry flounder pMCH (sf-pMCH) with physiological color change, blind-side malpigmentation, and feeding by quantifying mRNA expression level. Sf-pMCH1 cDNA had a 387-bp open reading frame (ORF) that encoded a protein consisting of 129 amino acid residues. The sf-pMCH1 protein included a signal peptide composed of 24 amino acid residues; MCH1 encoded a protein consisting of 17 amino acids. The sf-pMCH2 cDNA had a 450-bp ORF that encoded a protein consisting of 150 amino acid residues, which included a signal peptide comprising 23 amino acid residues; MCH2 encoded a protein consisting of 23 amino acids that was structurally similar to mammalian MCH. Reverse transcription-polymerase chain reaction (RT-PCR) revealed that the strongest sf-pMCHs gene expression was observed in the brain and pituitary, but weak or no amplification was detected in other tissues. The expression of sf-pMCH1 was relatively high compared to that of sf-pMCH2 in the brain. The relative levels of mRNA were significantly lower in dark background-reared and hypermelanic fish, indicating that the two pMCHs and background color are related to the physiological and morphological color changes of skin. In term of feeding regulation, we found an obvious functional role of pMCH1 in appetite, whereas the pMCH2 gene was not found to play a role in feeding. Copyright © 2013 Elsevier Inc. All rights reserved.

  15. Size selection in codends made of thin-twined Dyneema netting compared to standard codends: A case study with cod, plaice and flounder

    DEFF Research Database (Denmark)

    Herrmann, Bent; Wienbeck, Harald; Stepputis, D.

    2015-01-01

    In an experimental trawl fishery, diamond mesh codends made out of 2.5 mm flexible Dyneema twinewere tested. The principle aim was to investigate the effect of the number of meshes in the codendcircumference, the number of twines (single or double), and netting orientation (T0 or T90) on the size......In an experimental trawl fishery, diamond mesh codends made out of 2.5 mm flexible Dyneema twinewere tested. The principle aim was to investigate the effect of the number of meshes in the codendcircumference, the number of twines (single or double), and netting orientation (T0 or T90......) on the sizeselection of cod (Gadus morhua), plaice (Pleuronectes platessa) and flounder (Platichthys flesus). In addition,the obtained size selectivity for the codends made of Dyneema netting were compared to results obtainedfor T90 codends made of standard 5 mm single twine PE netting, and to previous results...... for other PEcodends used in the same fishery. It was observed that the selective performance of the Dyneema nettingcodends was very high compared to other T0 and T90 codends of the same mesh size and number ofmeshes in the codends circumference. This demonstrates the high selective potential of the thin...

  16. Latitudinal trends in habitat quality of shallow-water flatfish nurseries

    OpenAIRE

    Freitas, V.; Kooijman, S A L M; van der Veer, H.W.

    2012-01-01

    The habitat quality of European shallow-water nurseries was studied for 3 common flatfish species based on juvenile growth conditions. Field growth of 0-group plaice Pleuronectes platessa, flounder Platichthys flesus and sole Solea solea, from both published and unpublished studies, was compared with maximum growth predicted by a bioenergetics model based on the dynamic energy budget theory. In plaice and flounder, realized growth ratio decreased consistently during the growing season in most...

  17. Latitudinal trends in habitat quality of shallow-water flatfish nurseries

    NARCIS (Netherlands)

    Freitas, V.; Kooijman, S.A.L.M.; van der Veer, H.W.

    2012-01-01

    The habitat quality of European shallow-water nurseries was studied for 3 common flatfish species based on juvenile growth conditions. Field growth of 0-group plaice Pleuronectes platessa, flounder Platichthys flesus and sole Solea solea, from both published and unpublished studies, was compared

  18. Genetic differentiation in Japanese flounder in the Yellow Sea and ...

    African Journals Online (AJOL)

    The population structure of Japanese flounder (Paralichthys olivaceus) in the Yellow and East China Seas were analyzed using amplified fragment length polymorphism (AFLP) and cytochrome c oxidase subunit I (COI) gene sequencing. A total of 390 reproducible bands were generated by 10 AFLP primer combinations in ...

  19. Genetic variation and population structure of willowy flounder ...

    African Journals Online (AJOL)

    The first hypervariable region (HVR-1) of the mitochondrial DNA control region was utilized for determination of genetic variation and population structure in willowy flounder (Tanakius kitaharai) collected from Aomori, Ibaraki and Niigata. A total of 35 haplotypes were detected among 66 individuals with a total of 30 variable ...

  20. Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus).

    Science.gov (United States)

    Liu, Q H; Ma, D Y; Xu, S H; Xiao, Z Z; Xiao, Y S; Song, Z C; Li, J

    2015-03-01

    The present study established an efficient technology for summer flounder (Paralichthys dentatus) sperm cryopreservation, and successfully applied the cryopreserved sperm into interspecific hybridization with olive flounder (P olivaceus). The best motility of postthaw sperm (78.00 ± 4.70% and 76.60 ± 7.90%), fertilization rates (95.70 ± 3.60% and 79.40 ± 5.20%), and hatching rates (93.10 ± 4.00% and 77.20 ± 2.90%) were achieved when using propylene glycol or DMSO as cryoprotectant. Furthermore, we have successfully improved the cryopreservation method from using 2-mL cryotube to 5-mL cryotube, and the dilution ratio has been increased to 4:1. By this method, the cryopreservation efficiency has been improved to 30 times of that with routine method. Computer-assisted sperm motion analysis showed the freezing-thawing process decreased the sperm speed but did not significantly change the sperm movement pattern, and the progressive linear motion still was the dominant movement pattern. The ultrastructural analysis showed 50% to 60% of the spermatozoa had normal morphology, 20% to 30% were slightly damaged, such as swelling or rupture of head, midpiece, and tail region, and 10% to 20% were severely damaged. In the artificial hybridization experiment, high fertilization rates and hatching rates were achieved when using 15% DMSO (95.7 ± 3.6% and 79.4 ± 5.2%, respectively) and 15% propylene glycol (93.1 ± 4.0% and 77.2 ± 2.9%, respectively), with no significant difference in comparison with control (94.7 ± 2.6% and 72.5 ± 6.5%, respectively). In addition, we found the embryos and larvae from postthaw sperm of P dentatus developed and grew normally. The results of the present study further validated the safety of the cryopreserved sperm in breeding production by assessing the fertilization capacity, embryo development, and larval growth. Copyright © 2015 Elsevier Inc. All rights reserved.

  1. Transcriptional responses of olive flounder (Paralichthys olivaceus to low temperature.

    Directory of Open Access Journals (Sweden)

    Jinwei Hu

    Full Text Available The olive flounder (Paralichthys olivaceus is an economically important flatfish in marine aquaculture with a broad thermal tolerance ranging from 14 to 23°C. Cold-tolerant flounder that can survive during the winter season at a temperature of less than 14°C might facilitate the understanding of the mechanisms underlying the response to cold stress. In this study, the transcriptional response of flounder to cold stress (0.7±0.05°C was characterized using RNA sequencing. Transcriptome sequencing was performed using the Illumina MiSeq platform for the cold-tolerant (CT group, which survived under the cold stress; the cold-sensitive (CS group, which could barely survive at the low temperature; and control group, which was not subjected to cold treatment. In all, 29,021 unigenes were generated. Compared with the unigene expression profile of the control group, 410 unigenes were up-regulated and 255 unigenes were down-regulated in the CT group, whereas 593 unigenes were up-regulated and 289 unigenes were down-regulated in the CS group. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that signal transduction, lipid metabolism, digestive system, and signaling molecules and interaction were the most highly enriched pathways for the genes that were differentially expressed under cold stress. All these pathways could be assigned to the following four biological functions for flounder that can survive under cold stress: signal response to cold stress, cell repair/regeneration, energy production, and cell membrane construction and fluidity.

  2. Identification and differential expression of microRNAs during metamorphosis of the Japanese flounder (Paralichthys olivaceus.

    Directory of Open Access Journals (Sweden)

    Yuanshuai Fu

    Full Text Available BACKGROUND: MicroRNAs (miRNAs are a class of endogenous small non-coding RNAs of 20-25 nucleotides that play a key role in diverse biological processes. Japanese flounder undergo dramatic metamorphosis in their early development. The metamorphosis is characterized by morphological transformation from a bilaterally symmetrical to an asymmetrical body shape concomitant with extensive morphological and physiological remodeling of organs. So far, only a few miRNAs have been identified in fish and there are very few reports about the Japanese flounder miRNA. METHODOLOGY/PRINCIPAL FINDINGS: Solexa sequencing technology was used to perform high throughput sequencing of the small RNA library from the metamorphic period of Japanese flounder. Subsequently, aligning these sequencing data with metazoan known miRNAs, we characterized 140 conserved miRNAs and 57 miRNA: miRNA* pairs from the small RNA library. Among these 57 miRNA: miRNA* pairs, twenty flounder miRNA precursors were amplified from genomic DNA. We also demonstrated evolutionary conservation of Japanese flounder miRNAs and miRNA* in the animal evolution process. Using miRNA microarrays, we identified 66 differentially expressed miRNAs at two metamorphic stages (17 and 29 days post hatching of Japanese flounder. The results show that miRNAs might play a key role in regulating gene expression during Japanese flounder metamorphosis. CONCLUSIONS/SIGNIFICANCE: We identified a large number of miRNAs during flounder metamorphosis, some of which are differentially expressed at two different metamorphic stages. The study provides an opportunity for further understanding of miRNA function in the regulation of flounder metamorphosis and gives us clues for further studies of the mechanisms of metamorphosis in Japanese flounder.

  3. Effect of dietary carbohydrate sources on apparent nutrient digestibility of olive flounder (Paralichthys olivaceus feed

    Directory of Open Access Journals (Sweden)

    Md Mostafizur Rahman

    2016-06-01

    Full Text Available Abstract Apparent digestibility coefficients (ADCs of dry matter, crude protein, crude lipid, nitrogen-free extract, and energy in selected carbohydrate sources including wheat flour (WF, α-potato starch (PS, α-corn starch (CS, Na alginate (AL, dextrin (DEX, and carboxymethyl cellulose (CMC were determined for olive flounder. The olive flounder averaging 150 ± 8.0 g were held in 300-L tanks at a density of 30 fish per tank. Chromic oxide was used as the inert marker. Feces were collected from the flounder by a fecal collector attached to a fish rearing tank. Apparent dry matter and energy digestibilities of flounder fed WF, PS, CS, and DEX diets were significantly higher than those of fish fed AL and CMC diets. Apparent crude protein digestibility coefficients of flounder fed PS and CS diets were significantly higher than those of fish fed AL, DEX, and CMC diets. Apparent crude lipid and nitrogen-free extract digestibility coefficients of flounder fed PS and DEX diets were significantly higher than those of fish fed WF, CS, AL, and CMC diets. The present findings indicate that PS and DEX could be effectively used as dietary carbohydrate energy compared to WF, CS, AL, and CMC for olive flounder.

  4. Viral diseases of olive flounder in Korean hatcheries

    Science.gov (United States)

    Oh, M.-J.; Jung, S.-J.; Kitamura, S.-I.; Kim, H.-Y.; Kang, S. Y.

    2006-01-01

    In order to elucidate the state of diseases, especially viral diseases, and to prevent viral diseases from occurring in olive flounder hatcheries, a range of studies, including epidemiological study, were performed from 1997 to 2003. The location of the hatcheries investigated includes several representative sites in the east (Kangnung, Uljin, Pohang, Yangsan, Ulsan, Pusan), south (Wando, Changheung, Goheung, Yeosu, Namhae, Tongyeong, Geoje, Jeju) and west (Seosan, Kunsan, Gochang, Yeongkwang, Mokpo, Chindo) costal areas of the Korea Peninsula. A total of 2000 cases have been examined in 7 years, in which mortality caused by viral agents accounts for 22%, or 446 cases. Mortalities associated with viral infection considerably increased from 14% in 1997 to 27% in 2003. A variety of viral diseases were observed, and the occurrences of viral epidermal hyperplasia, viral ascites and viral deformity, viral nervous necrosis, and hirame rhabdoviral disease are 14%, 51%, 25%, and 8% respectively. By investigating the viral infection of broodstock flounder, the infection rate of marine birnavirus (MABV) in hatcheries was identified to be approximately 30%, therefore, it is highly necessary to acquire and keep non-infected broodstock fishes.

  5. Inactivation of Kudoa septempunctata in olive flounder meat by liquid freezing.

    Science.gov (United States)

    Ohnishi, Takahiro; Akuzawa, Sayuri; Furusawa, Hiroko; Yoshinari, Tomoya; Kamata, Yoichi; Sugita-Konishi, Yoshiko

    2014-01-01

    Kudoa septempunctata in olive flounder meat was inactivated using 3 distinct freezing methods:liquid freezing for 5 min, air blast freezing at -30℃ for 5 h, and -80℃ for 1 h. The fracture curve of olive flounder meat subjected to liquid freezing resembled that of meat stored at 4℃, indicating that the structure of olive flounder muscle was well preserved. In contrast, air blast freezing induced the disappearance of the fracture point in the fracture curve, indicating that there was deterioration in the meat quality. Liquid freezing preserved the transparency of olive flounder meat to the same degree as that of meat stored at 4°C. However, air blast freezing induced meat cloudiness. These results indicate that liquid freezing can be used for K. septempunctata inactivation without affecting the meat quality.

  6. Gonadal Transcriptome Analysis of Male and Female Olive Flounder (Paralichthys olivaceus

    Directory of Open Access Journals (Sweden)

    Zhaofei Fan

    2014-01-01

    Full Text Available Olive flounder (Paralichthys olivaceus is an important commercially cultured marine flatfish in China, Korea, and Japan, of which female grows faster than male. In order to explore the molecular mechanism of flounder sex determination and development, we used RNA-seq technology to investigate transcriptomes of flounder gonads. This produced 22,253,217 and 19,777,841 qualified reads from ovary and testes, which were jointly assembled into 97,233 contigs. Among them, 23,223 contigs were mapped to known genes, of which 2,193 were predicted to be differentially expressed in ovary and 887 in testes. According to annotation information, several sex-related biological pathways including ovarian steroidogenesis and estrogen signaling pathways were firstly found in flounder. The dimorphic expression of overall sex-related genes provides further insights into sex determination and gonadal development. Our study also provides an archive for further studies of molecular mechanism of fish sex determination.

  7. Germ cells and fertilization differences among Japanese flounder Paralichthys olivaceus, summer flounder Paralichthys dentatus and their first and second generations.

    Science.gov (United States)

    Sui, J; Ma, D Y; Liu, Q H; Xu, S H; Xiao, Z Z; Lin, F; Xiao, Y S; Li, J

    2012-03-01

    The morphology of gametes and the fertilization biology of Japanese flounder Paralichthys olivaceus (Po), summer flounder Paralichthys dentatus (Pd) and their hybrids were examined. Multiple generations (two hybrids: Po♀× Pd♂ (F1) and Pd♀× Po♂; two backcrosses: F1♀× Po♂ and F1♀× Pd♂) were obtained by artificial insemination. Comparison of egg ultrastructure among Po, Pd and F1 showed the morphology of micropyle region and the distribution density of pores were species specific. There were c. 100-200 accessory openings around the micropyle in Po, but not in Pd and F1. The zona radiata thickness and number of parallel bands were similar between F1 and Po, which were different from Pd. Comparison of spermatozoa ultrastructure revealed a close relationship between Po and Pd. Cytologically, the six crosses obeyed normal fertilization and cleavage processes, and only one male pronucleus was observed in a fertilized egg, indicating a monospermic fertilization pattern. Analysis of the time distribution from fertilization to first cleavage revealed an obvious delay at pronucleus fusion in the Pd × Po cross. The delay might indicate some cytoplasmic-nuclear incompatibility during the process of fertilization. © 2012 The Authors. Journal of Fish Biology © 2012 The Fisheries Society of the British Isles.

  8. Mercury accumulation and the mercury-PCB-sex interaction in summer flounder

    Science.gov (United States)

    Madenjian, Charles P.; Jensen, Olaf P.; Krabbenhoft, David P.; DeWild, John F.; Ogorek, Jacob M.; Vastano, Anthony R.

    2016-01-01

    Patterns in the relative differences in contaminant concentrations between the sexes of mature fish may reveal important behavioral and physiological differences between the sexes. We determined whole-fish total mercury (Hg) concentrations in 23 female summer flounder (Paralichthys dentatus) and 27 male summer flounder from New Jersey coastal waters. To estimate the change in Hg concentration due to release of eggs at spawning, Hg concentration in the somatic tissue and ovaries of 5 of the 23 female summer flounder were also determined. To ascertain whether most of the Hg in the summer flounder was methylmercury (MeHg), whole-fish MeHg concentrations were determined in all 50 summer flounder. Whole-fish Hg concentrations averaged 113 ng/g for females and 111 ng/g for males. Thus, females were 2% higher in Hg concentration than males, on average, but the difference was not statistically significant. Based on Hg determinations in the somatic tissue and ovaries, we predicted that Hg concentration of females would increase by 3.6%, on average, immediately after spawning due to release of eggs. On average, 92% of the Hg in the summer flounder was MeHg. To determine whether the effect of sex on Hg concentration was significantly different from the effect of sex on polychlorinated biphenyl (PCB) concentration, we paired our Hg determinations with PCB determinations from a previous study, and applied regression analysis. Sex significantly interacted with contaminant type (Hg or PCBs), as males were 43% higher in PCB concentration than females, whereas females were 2% higher in Hg concentration than males. Males eliminating Hg from their bodies at a faster rate than females was a likely explanation for this discrepancy between the two contaminant types. Overall, the Hg and PCB concentrations in the summer flounder were relatively low, and therefore our findings also had implications for continued operation of the summer flounder fishery.

  9. Vertebral deformities in hatchery-reared and wild-caught juvenile Japanese flounder, Paralichthys olivaceus

    Science.gov (United States)

    Lü, Hongjian; Zhang, Xiumei; Fu, Mei; Xi, Dan; Su, Shengqi; Yao, Weizhi

    2015-01-01

    The present study compared vertebral deformities of hatchery-reared and wild-caught juvenile Japanese flounder, Paralichthys olivaceus. A total of 362 hatchery-reared flounder (total length 122.5-155.8 mm) were collected from three commercial hatcheries located in Yantai, East China, and 89 wild fish (total length 124.7-161.3 mm) were caught off Yangma Island near Yantai City (37°27'N, 121°36'E). All the fish were dissected, photographed, and images of the axial skeleton were examined for vertebral deformities. Compared with wild-caught flounder in which no deformed vertebrae were detected, 48 (13.3%) hatcheryreared fish had deformed vertebrae. The deformities were classified as compression, compression-ankylosis, and dislocation-ankylosis. The vertebral deformities were mainly localized between post-cranial vertebra 1 and 3, with vertebrae number 1 as the most commonly deformed. The causative factors leading to vertebral deformities in reared Japanese flounder may be related to unfavorable temperature conditions, inflammation, damage, or rupture to the intervertebral ligaments under rearing conditions. Furthermore, no significant difference in the total number of vertebral bodies was observed between wild-caught (38.8±0.4) and hatchery-reared flounder (38.1±0.9) ( P>0.05). However, the number of vertebral bodies of hatchery-reared and wild-caught flounder ranged from 35 to 39 and from 38 to 39, respectively.

  10. Spread of Neoheterobothrium hirame (Monogenea), a serious pest of olive flounder Paralichthys olivaceus, to Korea.

    Science.gov (United States)

    Haywar, C J; Ki, J H; Heo, G J

    2001-08-02

    Neoheterobothrium hirame is a large, blood-feeding gill-worm infecting the highly prized olive flounder Paralichthys olivaceus in Japan. There is strong evidence that this worm is the primary cause of anaemia, a common and serious condition causing losses among both wild and cultured olive flounders. N. hirame was first detected in Japanese waters less than a decade ago, and its population then proliferated and spread throughout most of Japan, except Hokkaido. In neighbouring Korea, olive flounder is the most important species of cultured marine fish, and production currently exceeds that in Japan. However, until now, there have been no reports of any monogeneans or anaemia among olive flounders in Korea. Our survey conducted in 2000 of 100 cultured individuals from 4 provinces revealed 2 immature specimens of N. hirame: 1 from a land-based pond-culture system in southern Cheju Island (off the SW coast of Korea) and the other from a floating net cage near Yosu (in the mid-S part of the peninsula). The geographic range of this pathogen may have been enlarged as a result of introduction(s) of infected broodstock from Japan, but this seems unlikely. (The raising of this species in hatcheries developed in Korea in 1985, 7 years before the earliest detection of the worm in Japan.) Low numbers of flounders were also clearly anaemic. This, and the current rarity of N. hirame in Korean farms, appears to favour the hypothesis of a more recent, natural dispersal of the worm, during migrations of infected flounder across the narrow and shallow Tsushima and Korea Straits. Regardless of route of entry, we expect this pathogen will have an impact on Korean flounder fisheries equally serious to that being experienced in Japan.

  11. Osmoregulation and expression of ion transport proteins and putative claudins in the gill of southern flounder (Paralichthys lethostigma)

    DEFF Research Database (Denmark)

    Tipsmark, Christian K; Luckenbach, J Adam; Madsen, Steffen S

    2008-01-01

    The southern flounder is a euryhaline teleost that inhabits ocean, estuarine, and riverine environments. We investigated the osmoregulatory strategy of juvenile flounder by examining the time-course of homeostatic responses, hormone levels, and gill Na(+),K(+)-ATPase and Na(+),K(+),2Cl(-) cotrans...

  12. The toxic mechanism of high lethality of herbicide butachlor in marine flatfish flounder, Paralichthys olivaceus

    Science.gov (United States)

    Guo, Huarong; Yin, Licheng; Zhang, Shicui; Feng, Wenrong

    2010-09-01

    The toxic mechanism of herbicide butachlor to induce extremely high lethality in marine flatfish flounder, Paralichthys Olivaceus, was analyzed by histopathological examination, antioxidant enzymes activities and ATP content assay. Histopathological examination of gill, liver and kidney of exposed fishes showed that gill was a target organ of butachlor. The butachlor seriously impaired the respiration of gills by a series of lesions such as edema, lifting and detachment of lamellar epithelium, breakdown of pillar cells, and blood congestion. The dysfunction of gill respiration caused suffocation to the exposed flounder with extremely high acute lethality. Antioxidant enzyme activity assay of the in vitro cultured flounder gill (FG) cells exposed to butachlor indicated that butachlor markedly inhibited the antioxidant enzyme activities of Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX). Furthermore, along with the decline of antioxidant enzyme activities, ATP content in the exposed FG cells decreased, too. This infers that the oxidative stress induced by butachlor can inhibit the production of cellular ATP. Similar decrease of ATP content was also observed in the exposed flounder gill tissues. Taken together, as in FG cells, butachlor possibly induced a short supply of ATP in pillar cells by inhibiting the antioxidant enzyme activities and then affecting the contractibility of the pillar cells, which in turn resulted in the blood congestion and suffocation of exposed flounder.

  13. Pollutant body burdens and reproduction in Platichthys stellatus from San Francisco Bay. Annual progress report, year I

    Energy Technology Data Exchange (ETDEWEB)

    Spies, R.; Rice, D.; Ireland, R.; Beach, J.

    1983-08-01

    The effects of sublethal levels of pollutants in the tissues of estuarine fish on reproductive success were determined. The relationships among several measures of reproductive success (percent fertilization, hatching success, abnormal larvae) and biochemical measures of pollutant exposure (induced mixed-function oxidase) as well as direct measures of chemical pollutants in tissues were examined. Populations were sampled during active gametogenesis and during the spawning season. Tissues have been preserved for pollutant analyses. The reproductive success of captured spawning females was evaluated, and mixed-function oxidase (MFO) activity measured in captured fish. The results indicate the reproductive success of starry flounder in San Francisco Bay is being impaired by organic pollutants. 14 references, 8 figures, 11 tables.

  14. Is the World Floundering or Has She a Vision?

    Directory of Open Access Journals (Sweden)

    Ashok Natarajan

    2015-05-01

    Full Text Available To all appearances the world seems to be floundering without leadership, direction or even a clear formulation of goals and processes. Yet, paradoxically, amidst the apparent chaos and confusion, evolutionary patterns of advance somehow seem to emerge, universal values become ever more prevalent and powerful as deep drivers and determinates, a more than conventional wisdom seems to guide situations where conventional wisdom is stymied or blinded by dogma and superstition, and susceptible ignorant masses sooner or later choose a course that leads to the future rather than back to the past. This progressive movement is far from steady and often interspersed with retrograde steps and descends into utter folly and vicious violence, but all the same a direction seems to emerge that defies the expectations of scientific projections and doomsday prophets. Despite our adoration of rationality, irrationality prevails even in the halls of knowledge. Bursts of extraordinary creativity follow episodes of suicidal stupidity. Humanity’s predilection to self-destruction exists side by side with a serendipitous capacity for creativity and coming to our senses just in the nick of time to avert calamity. Pessimists decry the negative tendency. Optimists affirm the positive. None seem able to discern the pattern or process by which challenges become opportunities and imminent disaster is transmuted into progress. A key to deciphering this enigma lies in the invisible, yet to be realized potentials of the individual and society. This clumsy, costly, painful process is the mark of a still largely unconscious species struggling to discover its raison d’être and the secret of conscious evolution. Utopian ideals discredited by a world disillusioned by false promises exhibit a remarkable regenerative power to advance toward unseen goals. An unrealized vision founded on universal values guides us toward an inevitable destiny we have yet to conceive. Those seed

  15. Receptivity of winter flounder larvae to artificial diet from the yolk-sac stage to metamorphosis

    DEFF Research Database (Denmark)

    Butts, Ian; Ben Khemis, I.; Litvak, Matthew Kenneth

    2015-01-01

    In the period from 4 days post-hatching to metamorphosis, winter flounder that were naïve to artificial feed were exposed to an artificial diet and allowed to forage for 8 min. The presence or absence of artificial diet in the gut was used as an indicator of acceptance. The relationship between d...

  16. CONSUMPTIONS RATES OF SUMMER FLOUNDER LARVAE ON ROTIFER AND BRINE SHRIMP PREY DURING LARVAL REARING

    Science.gov (United States)

    Larval summer flounder Paralichthys dentatus were hatched and reared through metamorphosis in the laboratory. At several points in the rearing cycle, larvae were removed from their rearing chambers and placed in small bowls, where they were fed known quantities of the rotifer Bra...

  17. Responses of juvenile southern flounder exposed to Deepwater Horizon oil-contaminated sediments.

    Science.gov (United States)

    Brown-Peterson, Nancy J; Krasnec, Michelle O; Lay, Claire R; Morris, Jeffrey M; Griffitt, Robert J

    2017-04-01

    The Deepwater Horizon oil spill released millions of barrels of crude oil into the northern Gulf of Mexico, much of which remains associated with sediments and can have continuing impacts on biota. Juvenile southern flounder (Paralichthys lethostigma) were exposed for 28 d in the laboratory under controlled conditions to reference and Deepwater Horizon oil-contaminated sediments collected from coastal Louisiana to assess the impacts on an ecologically and commercially important benthic fish. The measured polycyclic aromatic hydrocarbon (PAH) concentrations in the sediments ranged from 0.25 mg/kg to 3940 mg/kg suite of 50 PAH analytes (tPAH50). Mortality increased with both concentration and duration of exposure. Exposed flounder length and weight was lower compared to controls after 28 d of exposure to the sediments with the highest PAH concentration, but condition factor was significantly higher in these fish compared with all other treatments. Histopathological analyses showed increased occurrence of gill abnormalities, including telangiectasis, epithelial proliferation, and fused lamellae in flounder exposed to sediments with the highest tPAH50 concentrations. In addition, hepatic vascular congestion and macrovesicular vacuolation were observed in flounder exposed to the more contaminated sediments. These data suggest that chronic exposure to field collected oil-contaminated sediments results in a variety of sublethal impacts to a benthic fish, with implications for long-term recovery from oil spills. Environ Toxicol Chem 2017;36:1067-1076. © 2016 SETAC. © 2016 SETAC.

  18. Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder

    Indian Academy of Sciences (India)

    In 6-month-old flounders, the expression of RAG1 and RAG2 was essentially restricted to the pronephros (head kidney) and mesonephros (truck kidney). Additionally, both of them were mainly expressed in the thymus. These results revealed that the thymus and kidney most likely serve as the primary lymphoid tissues in the ...

  19. 77 FR 76942 - 2013-2014 Summer Flounder and Scup Specifications; 2013 Black Sea Bass Specifications...

    Science.gov (United States)

    2012-12-31

    ... fishery quota and recreational harvest limit (RHL)), and research set-aside (RSA) established for the... lb (4,621 mt) for 2014. Consistent with the quota-setting procedures for the FMP, summer flounder...,000 \\1\\ Preliminary Research Set-Aside amount is 587,100 lb (266 mt). \\2\\ Kilograms are as converted...

  20. Stable carbon and oxygen isotope ratios of winter flounder otoliths assess connectivity between juvenile and adult habitats

    Science.gov (United States)

    Winter flounder populations (Pseudopleuronectes americanus) have significantly declined in recent years along the Rhode Island, USA coastline. The reasons for this decline are not completely clear; however, habitat loss may be a factor. Therefore, knowledge of connectivity betwee...

  1. Saptial and Temporal in Stable Carbon and Oxygen Isotope Ratios of Juvenile Winter Flounder Otoliths From Selected Nursery Areas

    Science.gov (United States)

    Winter flounder (Pseudopleuronectes americanus) populations have supported large commercial and recreational fisheries along the coast of New England. In recent years, however, the population of this important species has declined precipitously in some areas, especially Narragan...

  2. Differential expression of rhodopsin and Exo-rhodopsin genes in the retina and pineal gland of olive flounder (Paralichthys olivaceus)

    National Research Council Canada - National Science Library

    Shin, Hyun Suk; Kim, Na Na; Choi, Young Jae; Lee, Jehee; Kil, Gyung-Suk; Choi, Cheol Young

    2012-01-01

    ... (Exo-RH) genes. We isolated cDNAs of RH and Exo-RH genes from the retina and pineal gland, respectively, of the olive flounder and investigated the differential expression of these genes in these organs...

  3. Crude oil impairs immune function and increases susceptibility to pathogenic bacteria in southern flounder

    OpenAIRE

    Bayha, Keith M.; Ortell, Natalie; Ryan, Caitlin N.; Griffitt, Kimberly J.; Krasnec, Michelle; Sena, Johnny; Ramaraj, Thiruvarangan; Takeshita, Ryan; Gregory D Mayer; Schilkey, Faye; Griffitt, Robert J.

    2017-01-01

    Exposure to crude oil or its individual constituents can have detrimental impacts on fish species, including impairment of the immune response. Increased observations of skin lesions in northern Gulf of Mexico fish during the 2010 Deepwater Horizon oil spill indicated the possibility of oil-induced immunocompromisation resulting in bacterial or viral infection. This study used a full factorial design of oil exposure and bacterial challenge to examine how oil exposure impairs southern flounder...

  4. The efficacy of amoxicillin sodium against streptococcosis in cultured olive flounder Paralichthys olivaceus and its pharmacokinetics.

    Science.gov (United States)

    Lim, J-W; Jung, M-H; Jung, S-J; Kim, D-H; Park, K H; Kang, S Y

    2017-01-01

    The efficacy of amoxicillin sodium for controlling field and experimental Streptococcus iniae and S. parauberis infections in olive flounder (Paralichthys olivaceus) was evaluated after a single intramuscular administration. Furthermore, the minimal inhibitory concentrations (MIC) against 21 Streptococcus strains were determined. In addition, the pharmacokinetics and residue depletion in olive flounder were investigated. Single intramuscular doses of amoxicillin sodium at 20, 40, 80, and 160 mg/kg b.w. fish significantly reduced cumulative mortality rates to 18.8-31.3% (P amoxicillin sodium reduced the cumulative mortality rate to 7.5% and 4.8% at 20 and 40 mg/kg b.w. fish, respectively, whereas that of the untreated control group was 35.2%. Peak plasma concentrations (Cmax ) following a single intramuscular dose of 40 and 80 mg/kg b.w. fish were 62.64 (Tmax , 1.59 h) and 87.61 (Tmax , 3.02 h) μg/mL, respectively, with large AUC0-t /MIC and Cmax /MIC ratios, and sufficient T > MIC (time for maintaining plasma drug concentration greater than MICs) for S. iniae and S. parauberis. The estimated withdrawal period of amoxicillin sodium from muscle of olive flounder was about 8 days at 40 mg/kg b.w. fish (at 22 ± 1 °C). These results demonstrated a single intramuscular administration of amoxicillin sodium to be effective against streptococcosis in olive flounder. © 2016 John Wiley & Sons Ltd.

  5. Variations of T and B lymphocytes of flounder (Paralichthys olivaceus) after Hirame novirhabdovirus infection and immunization.

    Science.gov (United States)

    Xing, Jing; Wang, Lei; Zhen, Mengxiao; Tang, Xiaoqian; Zhan, Wenbin

    2018-02-16

    T and B lymphocytes are closely related to immunization and pathogen infection. Our previous study confirmed the CD3 + , CD4-1 + , CD4-2 + , CD8β + T lymphocytes and IgM + B lymphocytes presented in the peripheral blood leukocytes (PBLs) of flounder (Paralichthys olivaceus), in this paper, the variations of T and B lymphocytes of flounder after Hirame novirhabdovirus (HIRRV) infection or immunization were investigated. The flounders were injected with live or inactivated HIRRV, then the percentages of T and B lymphocytes in PBLs were analyzed by Flow cytometry (FCM), total antibodies and HIRRV-specific antibodies in serum were detected by Enzyme-linked immunosorbent assay (ELISA), and expression of twelve immune-related genes in the head kidneys were determined using q-PCR. The results showed that the percentages of CD3 + , CD4-1 + , CD4-2 + , CD8β + T lymphocytes and IgM + B lymphocytes significantly increased in both infection and immunization groups, in infection group they decreased rapidly after the peak and significantly lower than control levels at the end of infection, in immunization group they went down steadily to the control levels at the end of immunization. The total antibodies and HIRRV-specific antibodies increased first and peaked on the 7 th day post infection and on the 14 th day post immunization, respectively, then gradually decreased to the control levels. Additionally, twelve immune-related genes were up-regulated in both groups. These results demonstrated that the HIRRV induced both humoral and cellular immunity of flounder, the lymphocytes varied more sharply in infection group than those in immunization group and CD8 + T lymphocytes responded much more than CD4 + T lymphocytes to HIRRV antigen. Copyright © 2018 Elsevier Ltd. All rights reserved.

  6. Impact of elevated temperature on the growth, survival and trophic dynamics of winter flounder larvae: a mesocosm study

    Energy Technology Data Exchange (ETDEWEB)

    Keller, A. A.; Klein-MacPhee, G. [Rhode Island Univ., Narragansett, RI (United States)

    2000-12-01

    The impact of increased temperature on the growth, survival and trophic dynamics of winter flounder larvae was studied in a land-based mesocosm, in order to gain a better understanding of the factors controlling the recruitment of winter flounder, the dominant commercial fish in Narragansett Bay, Rhode Island. The investigation was prompted by a number of recent studies suggesting that the declining flounder population observed over the past 14 years was in some manner related to warmer winter temperatures which cause to increase the mortality of flounder larvae as well as predator activity. The paper describes the impact of increasing water temperature by three degrees relative to control systems in six enclosed mesocosms over a diatom post-winter-spring bloom period. The study focused on the effects of the altered temperature on food availability, abundance of active predators and the growth and survival of winter flounder larvae. It was observed that cooler temperature tended to prolong the incubation period of the larvae, resulting in hatching at a larger size in the cool mesocosm relative to the warm. Daily instantaneous growth and mortality rates showed a significant inverse relationship. The cumulative impact of warmer temperatures resulted in a 10 to 16 per cent decline of larvae surviving to metamorphosis (about six weeks). Increased temperature-mediated egg predation effects were also observed. It was concluded that chronic over-exploitation is associated with a long-term decline in winter flounder stock abundance despite production of good year-classes. Incorporation of the effect of warmer temperatures into fishing management plans, e. g. reducing fishing pressure following periods of successive warm winters, might be the most likely way to arrest declining stocks of winter flounder in the affected area. 34 refs., 4 tabs., 10 figs.

  7. In Vitro Inactivation of Kudoa septempunctata Spores Infecting the Muscle of Olive Flounder Paralichthys olivaceus.

    Science.gov (United States)

    Yokoyama, Hiroshi; Funaguma, Naoko; Kobayashi, Shoko

    2016-01-01

    Kudoa septempunctata, a myxosporean parasite infecting the trunk muscles of olive flounder (Paralichthys olivaceus), has been recently reported to be the causative agent of a type of food poisoning in humans. Patients exhibited acute diarrhea and vomiting after ingestion of the raw flesh of infected flounder. A recent increase in the number of food-poisoning cases has prompted us to develop a control strategy of this parasite. In this study, we evaluated the efficacy of several temperature and chemical treatments for inactivating K. septempunctata spores in vitro using the vital staining assay with the fluorescent dyes Hoechst 33342 and propidium iodide (PI). Screening tests of treatment methods against K. septempunctata suggested that 25% ethanol for 5 min, 80°C for 10 s, limonene at 10 μL/mL for 5 min, and salinities at 0‰ and 160‰ for 5 min were effective for killing spores. To verify toxicity loss in K. septempunctata spores after the treatments, tight junction barrier integrity assays with Caco-2 cells were conducted. The results of the Caco-2 assays corresponded well with those of the Hoechst 33342-PI staining assay. Further studies are required to determine a practical treatment procedure for inactivating spores considering the treatment application in the production process of cultured olive flounder.

  8. Polymorphysims of Gene in the Exons Were Associated with the Reproductive Endocrine of Japanese Flounder (

    Directory of Open Access Journals (Sweden)

    R. Q. Ma

    2012-06-01

    Full Text Available The cytochrome P450c17-I (CYP17-I is one of the enzymes critical to gonadal development and the synthesis of androgens. Two single nucleotide polymorphisms (SNPs were detected within the coding region of the CYP17-I gene in a population of 75 male Japanese flounder (Paralichthys olivaceus. They were SNP1 (c.C445T located in exon2 and SNP2 (c.T980C (p.Phe307Leu located in exon5. Four physiological indices, which were serum testosterone (T, serum 17β-estradiol (E2, Hepatosomatic index (HSI, and Gonadosomatic index (GSI, were studied to examine the effect of the two SNPs on the reproductive endocrines of Japanese flounder. Multiple comparisons revealed that CT genotype of SNP1 had a much lower T level than CC genotype (p<0.05 and the GSI of individuals with CC genotype of SNP2 was higher than those with TT genotype (p<0.05. Four diplotypes were constructed based on the two SNPs and the diplotype D3 had a significantly lower T level and GSI. In conclusion, the two SNPs were significantly associated with reproductive traits of Japanese flounder.

  9. Changes in Hematological, Biochemical and Non-specific Immune Parameters of Olive Flounder, , Following Starvation

    Directory of Open Access Journals (Sweden)

    Jong-Hyun Kim

    2014-09-01

    Full Text Available Triplicate groups of fed and starved olive flounder, Paralichthys olivaceus (body weight: 119.8±17.46 g, were examined over 42 days for physiological changes using hematological, biochemical, and non-specific immune parameters. No significant differences in concentrations of blood hemoglobin and hematocrit and plasma levels of total cholesterol, aspartate aminotransferase, alanine aminotransferase, glucose, and cortisol were detected between fed and starved groups at any sampling time throughout the experiment. In contrast, plasma total protein concentrations were significantly lower in starved fish than in fed fish from day 7 onwards. Moreover, plasma lysozyme concentrations were significantly higher in starved flounder from day 21 onwards. This result confirms that the response of olive flounder to short-term (less than about 1.5 months starvation consists of a readjustment of metabolism rather than the activation of an alarm-stress response. The present results indicate that starvation does not significantly compromise the health status of fish despite food limitation.

  10. A new PCR-based method shows that blue crabs (Callinectes sapidus (Rathbun)) consume winter flounder (Pseudopleuronectes americanus (Walbaum)).

    Science.gov (United States)

    Collier, Jackie L; Fitzgerald, Sean P; Hice, Lyndie A; Frisk, Michael G; McElroy, Anne E

    2014-01-01

    Winter flounder (Pseudopleuronectes americanus) once supported robust commercial and recreational fisheries in the New York (USA) region, but since the 1990s populations have been in decline. Available data show that settlement of young-of-the-year winter flounder has not declined as sharply as adult abundance, suggesting that juveniles are experiencing higher mortality following settlement. The recent increase of blue crab (Callinectes sapidus) abundance in the New York region raises the possibility that new sources of predation may be contributing to juvenile winter flounder mortality. To investigate this possibility we developed and validated a method to specifically detect winter flounder mitochondrial control region DNA sequences in the gut contents of blue crabs. A survey of 55 crabs collected from Shinnecock Bay (along the south shore of Long Island, New York) in July, August, and September of 2011 showed that 12 of 42 blue crabs (28.6%) from which PCR-amplifiable DNA was recovered had consumed winter flounder in the wild, empirically supporting the trophic link between these species that has been widely speculated to exist. This technique overcomes difficulties with visual identification of the often unrecognizable gut contents of decapod crustaceans, and modifications of this approach offer valuable tools to more broadly address their feeding habits on a wide variety of species.

  11. A new PCR-based method shows that blue crabs (Callinectes sapidus (Rathbun consume winter flounder (Pseudopleuronectes americanus (Walbaum.

    Directory of Open Access Journals (Sweden)

    Jackie L Collier

    Full Text Available Winter flounder (Pseudopleuronectes americanus once supported robust commercial and recreational fisheries in the New York (USA region, but since the 1990s populations have been in decline. Available data show that settlement of young-of-the-year winter flounder has not declined as sharply as adult abundance, suggesting that juveniles are experiencing higher mortality following settlement. The recent increase of blue crab (Callinectes sapidus abundance in the New York region raises the possibility that new sources of predation may be contributing to juvenile winter flounder mortality. To investigate this possibility we developed and validated a method to specifically detect winter flounder mitochondrial control region DNA sequences in the gut contents of blue crabs. A survey of 55 crabs collected from Shinnecock Bay (along the south shore of Long Island, New York in July, August, and September of 2011 showed that 12 of 42 blue crabs (28.6% from which PCR-amplifiable DNA was recovered had consumed winter flounder in the wild, empirically supporting the trophic link between these species that has been widely speculated to exist. This technique overcomes difficulties with visual identification of the often unrecognizable gut contents of decapod crustaceans, and modifications of this approach offer valuable tools to more broadly address their feeding habits on a wide variety of species.

  12. Variation in otolith macrostructure of Japanese flounder ( Paralichthys olivaceus): A method to discriminate between wild and released fish

    Science.gov (United States)

    Katayama, S.; Isshiki, T.

    2007-02-01

    The main objective of this study was to develop a method to discriminate between wild and hatchery-produced Japanese flounder, Paralichthys olivaceus, based on variations in otolith macrostructure. Otoliths of wild flounder were more elliptical than those of hatchery-produced fish, whereas otolith area and marginal coarseness showed no clear differences. Otolith morphometry did not vary significantly with water temperature or feeding conditions in rearing experiments. Reduced ellipticity in the otoliths of hatchery-produced fish could be caused by biotic and abiotic conditions after release. Throughout the study, it was found that otoliths of Japanese flounder reared at 15 and 20 °C regimes showed opaque zones regardless of feeding condition, while otolith of fish reared at 25 °C had translucent zones. The potential of thermal marks and secondary zones as a new mass-marking system is presented.

  13. Fecundity regulation in relation to habitat utilisation of two sympatric flounder (Platichtys flesus) populations in the brackish water Baltic Sea

    DEFF Research Database (Denmark)

    Nissling, Anders; Thorsen, Anders; da Silva, Filipa F.G.

    2015-01-01

    Two populations of flounder (Platichtys flesus) with different life history traits inhabit the brackish water Baltic Sea. Both types share feeding areas in coastal waters during summer-autumn but utilise different habitats for spawning in spring, namely offshore spawning with pelagic eggs...... to spawning in spring, and evaluated in relation to fish condition (Fulton's condition factor reflecting energy reserves of the fish) and feeding incidence of the respective population. Peaking in winter (December–February), fecundity regulation was significantly higher for coastal spawning flounder than...

  14. [Ecological groups of flatfishes of Peter the Great Bay (Japan sea) based on the analysis od species composition of myxosporidian parasites].

    Science.gov (United States)

    Antonenko, D V; Aseeva, N L

    2010-01-01

    Comparison of myxosporidian species compositions in different flatfish species fro Peter the Great Bay showed that the flatfishes should be divided into three ecological groups--deep water (scaly-eyed plaice Acanthopsetta nadeshnyi, Korean flounder Glyptocephalus stelleri, flatheaded flounder Hippoglossoides dubius, and pinewood flounder Cleisthenes herzensteini), relatively coastal (yellow-finned sole Limanda aspera and brown sole Pseudopleuronectes herzensteini), and coastal (Japanese flounder Pseudopleuronectes yokohamae, long-snouted flounder Limanda punctatissimus, starry flounder Platichthys stellatus, and white-bellied flounder Lepidopsetta mochigarei). This division coincides in general with the previously proposed ecological classification based on the characteristics of bathymetric distribution and preference to water layers during summer period in the fishes.

  15. Cloning, expression, and activity of type IV antifreeze protein from cultured subtropical olive flounder (Paralichthys olivaceus

    Directory of Open Access Journals (Sweden)

    Jong Kyu Lee

    2016-10-01

    Full Text Available Abstract Antifreeze proteins (AFPs lower the freezing point but not the melting point of aqueous solutions by inhibiting the growth of ice crystals via an adsorption-inhibition mechanism. However, the function of type IV AFP (AFP IV is questionable, as its antifreeze activity is on the verge of detectable limits, its physiological concentration in adult fish blood is too low to function as a biological antifreeze, and its homologues are present even in fish from tropic oceans as well as freshwater. Therefore, we speculated that AFP IV may have gained antifreeze activity not by selective pressure but by chance. To test this hypothesis, we cloned, expressed, and assayed AFP IV from cultured subtropical olive flounder (Paralichthys olivaceus, which do not require antifreeze protein for survival. Among the identified expressed sequence tags of the flounder liver sample, a 5′-deleted complementary DNA (cDNA sequence similar to the afp4 gene of the longhorn sculpin was identified, and its full-length cDNA and genome structure were examined. The deduced amino acid sequence of flounder AFP IV shared 55, 53, 52, and 49 % identity with those of Pleuragramma antarcticum, Myoxocephalus octodecemspinosus, Myoxocephalus scorpius, and Notothenia coriiceps, respectively. Furthermore, the genomic structure of this gene was conserved with those of other known AFP IVs. Notably, the recombinant AFP IV showed a weak but distinct thermal hysteresis of 0.07 ± 0.01 °C at the concentration of 0.5 mg/mL, and ice crystals in an AFP IV solution grew star-shaped, which are very similar to those obtained from other polar AFP IVs. Taken together, our results do not support the hypothesis of evolution of AFP IV by selective pressure, suggesting that the antifreeze activity of AFP IV may have been gained by chance.

  16. Differences in energy expenditures and growth dilution explain higher PCB concentrations in male summer flounder

    Science.gov (United States)

    Madenjian, Charles P.; Jensen, Olaf P.; Rediske, Richard R.; O'Keefe, James P.; Vastano, Anthony R.; Pothoven, Steven A.

    2016-01-01

    Comparison of polychlorinated biphenyl (PCB) concentrations between the sexes of mature fish may reveal important behavioral and physiological differences between the sexes. We determined whole-fish PCB concentrations in 23 female summer flounder Paralichthys dentatus and 27 male summer flounder from New Jersey coastal waters. To investigate the potential for differences in diet or habitat utilization between the sexes, carbon and nitrogen stable isotope ratios were also determined. In 5 of the 23 female summer flounder, PCB concentrations in the somatic tissue and ovaries were determined. In addition, we used bioenergetics modeling to assess the contribution of the growth dilution effect to the observed difference in PCB concentrations between the sexes. Whole-fish PCB concentrations for females and males averaged 87 and 124 ng/g, respectively; thus males were 43% higher in PCB concentration compared with females. Carbon and nitrogen stable isotope ratios did not significantly differ between the sexes, suggesting that diet composition and habitat utilization did not vary between the sexes. Based on PCB determinations in the somatic tissue and ovaries, we predicted that PCB concentration of females would increase by 0.6%, on average, immediately after spawning due to release of eggs. Thus, the change in PCB concentration due to release of eggs did not explain the higher PCB concentrations observed in males. Bioenergetics modeling results indicated that the growth dilution effect could account for males being 19% higher in PCB concentration compared with females. Thus, the bulk of the observed difference in PCB concentrations between the sexes was not explained by growth dilution. We concluded that a higher rate of energy expenditure in males, stemming from greater activity and a greater resting metabolic rate, was most likely the primary driver for the observed difference in PCB concentrations between the sexes.

  17. Differences in Energy Expenditures and Growth Dilution Explain Higher PCB Concentrations in Male Summer Flounder.

    Directory of Open Access Journals (Sweden)

    Charles P Madenjian

    Full Text Available Comparison of polychlorinated biphenyl (PCB concentrations between the sexes of mature fish may reveal important behavioral and physiological differences between the sexes. We determined whole-fish PCB concentrations in 23 female summer flounder Paralichthys dentatus and 27 male summer flounder from New Jersey coastal waters. To investigate the potential for differences in diet or habitat utilization between the sexes, carbon and nitrogen stable isotope ratios were also determined. In 5 of the 23 female summer flounder, PCB concentrations in the somatic tissue and ovaries were determined. In addition, we used bioenergetics modeling to assess the contribution of the growth dilution effect to the observed difference in PCB concentrations between the sexes. Whole-fish PCB concentrations for females and males averaged 87 and 124 ng/g, respectively; thus males were 43% higher in PCB concentration compared with females. Carbon and nitrogen stable isotope ratios did not significantly differ between the sexes, suggesting that diet composition and habitat utilization did not vary between the sexes. Based on PCB determinations in the somatic tissue and ovaries, we predicted that PCB concentration of females would increase by 0.6%, on average, immediately after spawning due to release of eggs. Thus, the change in PCB concentration due to release of eggs did not explain the higher PCB concentrations observed in males. Bioenergetics modeling results indicated that the growth dilution effect could account for males being 19% higher in PCB concentration compared with females. Thus, the bulk of the observed difference in PCB concentrations between the sexes was not explained by growth dilution. We concluded that a higher rate of energy expenditure in males, stemming from greater activity and a greater resting metabolic rate, was most likely the primary driver for the observed difference in PCB concentrations between the sexes.

  18. A second generation genetic linkage map of Japanese flounder (Paralichthys olivaceus

    Directory of Open Access Journals (Sweden)

    Okamoto Hiroyuki

    2010-10-01

    Full Text Available Abstract Background Japanese flounder (Paralichthys olivaceus is one of the most economically important marine species in Northeast Asia. Information on genetic markers associated with quantitative trait loci (QTL can be used in breeding programs to identify and select individuals carrying desired traits. Commercial production of Japanese flounder could be increased by developing disease-resistant fish and improving commercially important traits. Previous maps have been constructed with AFLP markers and a limited number of microsatellite markers. In this study, improved genetic linkage maps are presented. In contrast with previous studies, these maps were built mainly with a large number of codominant markers so they can potentially be used to analyze different families and populations. Results Sex-specific genetic linkage maps were constructed for the Japanese flounder including a total of 1,375 markers [1,268 microsatellites, 105 single nucleotide polymorphisms (SNPs and two genes]; 1,167 markers are linked to the male map and 1,067 markers are linked to the female map. The lengths of the male and female maps are 1,147.7 cM and 833.8 cM, respectively. Based on estimations of map lengths, the female and male maps covered 79 and 82% of the genome, respectively. Recombination ratio in the new maps revealed F:M of 1:0.7. All linkage groups in the maps presented large differences in the location of sex-specific recombination hot-spots. Conclusions The improved genetic linkage maps are very useful for QTL analyses and marker-assisted selection (MAS breeding programs for economically important traits in Japanese flounder. In addition, SNP flanking sequences were blasted against Tetraodon nigroviridis (puffer fish and Danio rerio (zebrafish, and synteny analysis has been carried out. The ability to detect synteny among species or genera based on homology analysis of SNP flanking sequences may provide opportunities to complement initial QTL experiments

  19. Occurrence and significance of atypical Aeromonas salmonicida in non-salmonid and salmonid fish species : A review

    DEFF Research Database (Denmark)

    Wiklund, T.; Dalsgaard, Inger

    1998-01-01

    . The best known diseases associated with atypical A. salmonicida are carp Cyprinus carpio erythrodermatitis, goldfish Carassius auratus ulcer disease, and ulcer disease of flounder Platichthys flesus, but atypical strains are apparently involved in more disease outbreaks than previously suspected...

  20. Quantitative comparison of the expression of myogenic regulatory factors in flounder ( Paralichthys olivaceus) embryos and adult tissues

    Science.gov (United States)

    Zhang, Yuqing; Tan, Xungang; Xu, Peng; Sun, Wei; Xu, Yongli; Zhang, Peijun

    2010-03-01

    MyoD, Myf5, and myogenin are myogenic regulatory factors that play important roles during myogenesis. It is thought that MyoD and Myf5 are required for myogenic determination, while myogenin is important for terminal differentiation and lineage maintenance. To better understand the function of myogenic regulatory factors in muscle development of flounder, an important economic fish in Asia, real-time quantitative RT-PCR was used to characterize the expression patterns of MyoD, Myf5, and myogenin at early stages of embryo development, and in different tissues of the adult flounder. The results show that, Myf5 is the first gene to be expressed during the early stages of flounder development, followed by MyoD and myogenin. The expressions of Myf5, yoD, and myogenin at the early stages have a common characteristic: expression gradually increased to a peak level, and then gradually decreased to an extremely low level. In the adult flounder, the expression of the three genes in muscle is much higher than that in other tissues, indicating that they are important for muscle growth and maintenance of grown fish. During embryonic stages, the expression level of MyoD might serve an important role in the balance between muscle cell differentiation and proliferation. When the MyoD expression is over 30% of its highest level, the muscle cells enter the differentiation stage.

  1. Characterization of flounder ( Paralichthys olivaceus) FoxD5 and its function in regulating myogenic regulatory factor

    Science.gov (United States)

    Tan, Xungang; Zhang, Yuqing; Sun, Wei; Zhang, Peijun; Xu, Yongli

    2012-03-01

    As one member of winged helix domain transcription factors, FoxD5 was reported to be a trunk organizer. Recent study showed that zebrafish foxd5 is expressed in the somites. To further understand the function of FoxD5 in fish muscle development, the FoxD5 gene was isolated from flounder. Its expression pattern was analyzed by in situ hybridization, while its function in regulating myogenic regulatory factor, MyoD, was analyzed by ectopic expression. It showed that flounder FoxD5 was firstly expressed in the tailbud, adaxial cells, and neural plate of the head. In flounder embryo, FoxD5 is expressed not only in forebrain but also in somite cells that will form muscle in the future. When flounder FoxD5 was over-expressed in zebrafish by microinjection, the expression of zebrafish MyoD in the somites was reduced, suggesting that FoxD5 is involved in myogenesis by regulating the expression of MyoD.

  2. Trends in nitrogen isotope ratios of juvenile winter flounder reflect changing nitrogen inputs to Rhode Island, USA estuarine systems

    Science.gov (United States)

    Nitrogen isotope ratios (d 15N) in juvenile winter flounder, Pseudopleuronectes americanus, were used to examine changes in nitrogen inputs to several Rhode Island, USA estuarine systems. Fish were collected over two three-year periods with a ten-year interval between sampling pe...

  3. Detection of lymphocystis disease virus in Japanese flounder Paralichthys olivaceus and other marine teleosts from northern China

    Science.gov (United States)

    Zhan, Wenbin; Li, Yongqin; Sheng, Xiuzhen; Xing, Jing; Tang, Xiaoqian

    2010-11-01

    We isolated a strain of lymphocystis disease virus (LCDV) from Japanese flounder ( Paralichthys olivaceus) cultured in northern China. Based on published sequences of major capsid protein (MCP) gene of LCDV-cn (GenBank: AF126405), we designed two primer sets P1/P2 and P3/P4. We then used one-step or nested PCR and in-situ hybridization (ISH) to detect LCDV and identify the target tissues or cells in infected Japanese flounder. The PCR products were positive in purified viral supernatant, skin nodules, gut, gill, kidney, spleen, stomach, heart, and liver of Japanese flounder. We compared the DNA sequence with 14 MCP nucleotide sequences from GenBank, including Megalocytivirus (OFIV and RSIV), Iridovirus (CzIV and WIV), Ranavirus (TFV and FV3), and Lymphocystivirus (8 LCDV). Based on the alignment, we confirmed the PCR product was from Lymphocystivirus (GenBank accession number DQ279090 (LCDV-HD)). Using ISH, we noted the presence of LCDV in the skin nodules, gut, gill, spleen, stomach, and heart of spontaneously infected Japanese flounders. We successfully amplified LCDV fragments from Schlegel’s black rockfish ( Sebastes schlegeli Higendorf), redwing sea robin ( Lepidotrigla microptera Günther) and turbot ( Scophthalmus maximus) using the one-step and nested PCR, suggesting the target genes can be widely detected in fish using this method.

  4. 77 FR 24151 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

    Science.gov (United States)

    2012-04-23

    ... are not overfished or subject to overfishing in 2012. This final rule makes no changes to the interim... Flounder The updated stock assessment overfishing limit (OFL) is 31,588,000 lb (14,328 mt). The projected... the Council's risk policy results in an overfishing risk tolerance (P*) of 0.40, or a 40- percent risk...

  5. Characterization of a specific monoclonal antibody against immunoglobulin light kappa/L1 chain in olive flounder (Paralichthys olivaceus)

    DEFF Research Database (Denmark)

    Kim, Young Kyu; Lee, Jung Seok; Jung, Jae Wook

    2017-01-01

    successfully to study immune responses in fish. There is therefore a need to produce Ig-specific cell markers for fish. Here, we attempted to identify the specific isotype detected by an Ig light chain-specific monoclonal antibody (anti-olive flounder IgL-mAb: M7C3-4) that we had previously produced [11......]. Three newly identified sequences of the Ig light chain from olive flounder were classified according to their isotypes. Subsequent analyses revealed that M7C3-4 was able to specifically detect lymphocytes expressing one of the κ chains (Igκ-a) in olive flounder. Interestingly, Igκ-a+ B cells were more...... abundant in spleen and trunk-kidney than in peripheral blood, indicating a distribution different from that of IgM+ B cells. Our work reveals interesting aspects of B cell distribution and differentiation, and may aid in the production of suitable and effective cell markers for olive flounder....

  6. Possible Involvement of Photoperiodic Regulation in Reproductive Endocrine System of Female Olive Flounder Paralichthys olivaceus.

    Science.gov (United States)

    Kim, Hyun Chul; Lee, Chi Hoon; Hur, Sung Pyu; Kim, Byeong Hoon; Park, Jun Young; Lee, Young Don

    2015-03-01

    This study investigated possible involvement of photoperiodic regulation in reproductive endocrine system of female olive flounder. To investigate the influence on brain-pituitary axis in endocrine system by regulating photoperiod, compared expression level of Kisspeptin and sbGnRH mRNA in brain and FSH-β, LH-β and GH mRNA in pituitary before and after spawning. Photoperiod was treated natural photoperiod and long photoperiod (15L:9D) conditions from Aug. 2013 to Jun. 2014. Continuous long photoperiod treatment from Aug. (post-spawning phase) was inhibited gonadal development of female olive flounder. In natural photoperiod group, the Kiss2 expression level a significant declined in Mar. (spawning period). And also, FSH-β, LH-β and GH mRNA expression levels were increasing at this period. However, in long photoperiod group, hypothalamic Kiss2, FSH-β, LH-β and GH mRNA expression levels did not show any significant fluctuation. These results suggest that expression of hypothalamic Kiss2, GtH and GH in the pituitary would change in response to photoperiod and their possible involvement of photoperiodic regulation in reproductive endocrine system of the BPG axis.

  7. Induction of antiviral state in fish cells by Japanese flounder, Paralichthys olivaceus, interferon regulatory factor-1.

    Science.gov (United States)

    Caipang, Christopher Marlowe A; Hirono, Ikuo; Aoki, Takashi

    2005-07-01

    Interferon regulatory factor-1 (IRF-1) mediates an antiviral state in cells by regulating the expression of the interferon (IFN-alpha/beta) system. To elucidate the role of IRF-1 in fish during virus infections, we constructed a recombinant plasmid of the Japanese flounder, Paralichthys olivaceus IRF-1 (JF IRF-1) under the control of the cytomegalovirus (CMV) immediate/early enhancer promoter. The antiviral mechanism of JF IRF-1 was studied using transfection experiments in a homologous cell line. Here, we show that cell supernatants obtained from transiently transfected cells enhanced cell viability of a heterologous cell line upon incubation, reduced the titers of hirame rhabdovirus (HIRRV) and viral hemorrhagic septicemia virus (VHSV), and possessed cytokine-like activity, as shown by their ability to protect cells against virus infections. The supernatants also inhibited the replication of the rhabdoviruses during the early stages of infection as indicated by the reduction of viral titers in the presence of the supernatants obtained from the transfected cells. Further analysis showed that the cell culture supernatants contain cytokine-like substances that possess acid-labile and temperature-resistant properties. These results indicate that JF IRF-1 induces an antiviral state in cells by mediating the production of cytokine-like substances. Thus, JF IRF-1 might be useful as an adjuvant in the development of DNA vaccines against commercially important viral pathogens in Japanese flounder aquaculture.

  8. Mycobacterium bovis BCG vaccine induces non-specific immune responses in Japanese flounder against Nocardia seriolae.

    Science.gov (United States)

    Kato, Goshi; Kondo, Hidehiro; Aoki, Takashi; Hirono, Ikuo

    2012-08-01

    Nocardiosis caused by Nocardia seriolae has been causing severe loss of fish production, so that an effective vaccine is urgently needed. Mycobacterium bovis BCG (BCG) is a live attenuated vaccine for tuberculosis, which is effective against various infectious diseases including nocardiosis in mammals. In this study, the protective efficacy of BCG against N. seriolae was evaluated in Japanese flounder Paralichthys olivaceus and antigen-specific immune responses induced in BCG vaccinated fish were investigated. Cumulative mortality of BCG-vaccinated fish was 21.4% whereas that of PBS-injected fish was 56.7% in N. seriolae challenge. However, gene expression level of IFN-γ was only slightly up-regulated in BCG-vaccinated fish after injection of N. seriolae antigen. In order to reveal non-specific immune responses induced by BCG vaccination, transcriptome of the kidney after BCG vaccination was investigated using oligo DNA microarray. Gene expression levels of antimicrobial peptides such as C-type and G-type lysozyme were significantly up-regulated after BCG vaccination. Consistently, BCG vaccination appeared to increase the bacteriolysis activity of the serum against Micrococcus luteus and N. seriolae. These results suggest that BCG-vaccinated Japanese flounder fight N. seriolae infection mainly by non-specific immune responses such as by the production of bacteriolytic lysozymes. Copyright © 2012 Elsevier Ltd. All rights reserved.

  9. Crude oil impairs immune function and increases susceptibility to pathogenic bacteria in southern flounder.

    Science.gov (United States)

    Bayha, Keith M; Ortell, Natalie; Ryan, Caitlin N; Griffitt, Kimberly J; Krasnec, Michelle; Sena, Johnny; Ramaraj, Thiruvarangan; Takeshita, Ryan; Mayer, Gregory D; Schilkey, Faye; Griffitt, Robert J

    2017-01-01

    Exposure to crude oil or its individual constituents can have detrimental impacts on fish species, including impairment of the immune response. Increased observations of skin lesions in northern Gulf of Mexico fish during the 2010 Deepwater Horizon oil spill indicated the possibility of oil-induced immunocompromisation resulting in bacterial or viral infection. This study used a full factorial design of oil exposure and bacterial challenge to examine how oil exposure impairs southern flounder (Paralichthys lethostigma) immune function and increases susceptibility to the bacteria Vibrio anguillarum, a causative agent of vibriosis. Fish exposed to oil prior to bacterial challenge exhibited 94.4% mortality within 48 hours of bacterial exposure. Flounder challenged with V. anguillarum without prior oil exposure had Oil/Pathogen challenged fish and was nearly non-existent in the No Oil/Pathogen challenged fish bacterial community. Elevated V. anguillarum levels were a direct result of oil exposure-induced immunosuppression. Oil-exposure reduced expression of immunoglobulin M, the major systemic fish antibody, and resulted in an overall downregulation in transcriptome response, particularly in genes related to immune function, response to stimulus and hemostasis. Ultimately, sediment-borne oil exposure impairs immune function, leading to increased incidences of bacterial infections. This type of sediment-borne exposure may result in long-term marine ecosystem effects, as oil-bound sediment in the northern Gulf of Mexico will likely remain a contamination source for years to come.

  10. Protective efficiency of an inactivated vaccine against Streptococcus iniae in olive flounder, Paralichthys olivaceus

    Directory of Open Access Journals (Sweden)

    Jeong Yong-Uk

    2016-03-01

    Full Text Available Streptococcus iniae is a causative agent of hemorrhagic septicemia in olive flounder, Paralichthys olivaceus, in Korea, resulting in serious economic losses. As a preventive measure, M VAC INIAE (Mastuken, Japan was prepared from the S. iniae F2K strain and tested against the SI-36 strain prevalent on flounder fish farms on Jeju Island, Korea. F2K had a serotype of 38 (− and SI-36 38 (+. The vaccine recognized both serotypes. It showed a very high effective immune response against S. iniae; the challenge test using the S. iniae SI-36 strain resulted in a relative percent survival (RPS of 85.7-87.0% 2 weeks after vaccination and 71.0-80.0% 6 months after vaccination. Field vaccination and clinical challenge tests were performed at local Jeju aquafarms with S. iniae SI-36. These showed significantly reduced cumulative mortality when compared to the control group with RPS rates that ranged between 71-80%. Hence, the present study suggests that this vaccine showed a significant immune response against S. iniae and could be applied in commercial aquafarms as a therapeutic agent against β-hemolytic streptococcosis in cultured P. olivaceus.

  11. Spatial and temporal variability in growth of southern flounder (Paralichthys lethostigma)

    Science.gov (United States)

    Midway, Stephen R.; Wagner, Tyler; Arnott, Stephen A.; Biondo, Patrick; Martinez-Andrade, Fernando; Wadsworth, Thomas F.

    2015-01-01

    Delineation of stock structure is important for understanding the ecology and management of many fish populations, particularly those with wide-ranging distributions and high levels of harvest. Southern flounder (Paralichthys lethostigma) is a popular commercial and recreational species along the southeast Atlantic coast and Gulf of Mexico, USA. Recent studies have provided genetic and otolith morphology evidence that the Gulf of Mexico and Atlantic Ocean stocks differ. Using age and growth data from four states (Texas, Alabama, South Carolina, and North Carolina) we expanded upon the traditional von Bertalanffy model in order to compare growth rates of putative geographic stocks of southern flounder. We improved the model fitting process by adding a hierarchical Bayesian framework to allow each parameter to vary spatially or temporally as a random effect, as well as log transforming the three model parameters (L∞, K, andt0). Multiple comparisons of parameters showed that growth rates varied (even within states) for females, but less for males. Growth rates were also consistent through time, when long-term data were available. Since within-basin populations are thought to be genetically well-mixed, our results suggest that consistent small-scale environmental conditions (i.e., within estuaries) likely drive growth rates and should be considered when developing broader scale management plans.

  12. Analysis of new microsatellite markers developed from reported sequences of Japanese flounder Paralichthys olivaceus

    Science.gov (United States)

    Yu, Haiyang; Jiang, Liming; Chen, Wei; Wang, Xubo; Wang, Zhigang; Zhang, Quanqi

    2010-12-01

    The expressed sequence tags (ESTs) of Japanese flounder, Paralichthys olivaceus, were selected from GenBank to identify simple sequence repeats (SSRs) or microsatellites. A bioinformatic analysis of 11111 ESTs identified 751 SSR-containing ESTs, including 440 dinucleotide, 254 trinucleotide, 53 tetranucleotide, 95 pentanucleotide and 40 hexanucleotide microsatellites respectively. The CA/TG and GA/TC repeats were the most abundant microsatellites. AT-rich types were predominant among trinucleotide and tetranucleotide microsatellites. PCR primers were designed to amplify 10 identified microsatellites loci. The PCR results from eight pairs of primers showed polymorphisms in wild populations. In 30 wild individuals, the mean observed and expected heterozygosities of these 8 polymorphic SSRs were 0.71 and 0.83 respectively and the average PIC value was 0.8. These microsatellite markers should prove to be a useful addition to the microsatellite markers that are now available for this species.

  13. In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus

    Science.gov (United States)

    Su, Feng; Zhang, Shicui; Li, Hongyan; Guo, Huarong

    2007-04-01

    In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid (IMI) to the gill cell line of flounder (FG) that collected in the gill of Paralichthys olivaceus, was examined by 3 widely used endpoint bioassays: NR (neutral red), MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and TCP (total cell protein). The result shows that the IMI increased at concentrations ≥0.5 μg/ml. The IC50 value of NR. MTT, and TCP was 41.86, 38.46, and 39.08 μg/ml, respectively. The ultrastructural observation revealed that the mitochondria of the cells exposed to 60 μg/ml IMI for 48 h were severely damaged, swollen or disrupted, while their nuclei and rough endoplasmic reticulum (RER) remained normal. This would suggest that the mitochondria are probably the primary target of IMI.

  14. Crude oil impairs immune function and increases susceptibility to pathogenic bacteria in southern flounder.

    Directory of Open Access Journals (Sweden)

    Keith M Bayha

    Full Text Available Exposure to crude oil or its individual constituents can have detrimental impacts on fish species, including impairment of the immune response. Increased observations of skin lesions in northern Gulf of Mexico fish during the 2010 Deepwater Horizon oil spill indicated the possibility of oil-induced immunocompromisation resulting in bacterial or viral infection. This study used a full factorial design of oil exposure and bacterial challenge to examine how oil exposure impairs southern flounder (Paralichthys lethostigma immune function and increases susceptibility to the bacteria Vibrio anguillarum, a causative agent of vibriosis. Fish exposed to oil prior to bacterial challenge exhibited 94.4% mortality within 48 hours of bacterial exposure. Flounder challenged with V. anguillarum without prior oil exposure had <10% mortality. Exposure resulted in taxonomically distinct gill and intestine bacterial communities. Mortality strongly correlated with V. anguillarum levels, where it comprised a significantly higher percentage of the microbiome in Oil/Pathogen challenged fish and was nearly non-existent in the No Oil/Pathogen challenged fish bacterial community. Elevated V. anguillarum levels were a direct result of oil exposure-induced immunosuppression. Oil-exposure reduced expression of immunoglobulin M, the major systemic fish antibody, and resulted in an overall downregulation in transcriptome response, particularly in genes related to immune function, response to stimulus and hemostasis. Ultimately, sediment-borne oil exposure impairs immune function, leading to increased incidences of bacterial infections. This type of sediment-borne exposure may result in long-term marine ecosystem effects, as oil-bound sediment in the northern Gulf of Mexico will likely remain a contamination source for years to come.

  15. De Novo assembly of the Japanese flounder (Paralichthys olivaceus spleen transcriptome to identify putative genes involved in immunity.

    Directory of Open Access Journals (Sweden)

    Lin Huang

    Full Text Available Japanese flounder (Paralichthys olivaceus is an economically important marine fish in Asia and has suffered from disease outbreaks caused by various pathogens, which requires more information for immune relevant genes on genome background. However, genomic and transcriptomic data for Japanese flounder remain scarce, which limits studies on the immune system of this species. In this study, we characterized the Japanese flounder spleen transcriptome using an Illumina paired-end sequencing platform to identify putative genes involved in immunity.A cDNA library from the spleen of P. olivaceus was constructed and randomly sequenced using an Illumina technique. The removal of low quality reads generated 12,196,968 trimmed reads, which assembled into 96,627 unigenes. A total of 21,391 unigenes (22.14% were annotated in the NCBI Nr database, and only 1.1% of the BLASTx top-hits matched P. olivaceus protein sequences. Approximately 12,503 (58.45% unigenes were categorized into three Gene Ontology groups, 19,547 (91.38% were classified into 26 Cluster of Orthologous Groups, and 10,649 (49.78% were assigned to six Kyoto Encyclopedia of Genes and Genomes pathways. Furthermore, 40,928 putative simple sequence repeats and 47, 362 putative single nucleotide polymorphisms were identified. Importantly, we identified 1,563 putative immune-associated unigenes that mapped to 15 immune signaling pathways.The P. olivaceus transciptome data provides a rich source to discover and identify new genes, and the immune-relevant sequences identified here will facilitate our understanding of the mechanisms involved in the immune response. Furthermore, the plentiful potential SSRs and SNPs found in this study are important resources with respect to future development of a linkage map or marker assisted breeding programs for the flounder.

  16. Characterization of Japanese flounder (Paralichthys olivaceus) Caspase1 involved in extracellular ATP-mediated immune signaling in fish.

    Science.gov (United States)

    Li, Shuo; Peng, Weijiao; Li, Jiafang; Hao, Gaixiang; Geng, Xuyun; Sun, Jinsheng

    2017-08-01

    Caspase1 is a member of inflammatory Caspases that play important roles in the innate immune system. Although several teleost caspase1 genes have been identified, their partner proteins and implication in extracellular ATP-mediated immune signaling in fish are still very limited. Here we identified and characterized a caspase1 gene, named JfCaspase1, from Japanese flounder Paralichthys olivaceus. JfCaspase1 mRNA was constitutively expressed in all examined normal tissues with high expression in skin and gills and moderate expression in the enriched Japanese flounder head kidney macrophages (HKMs) and peripheral blood leukocytes (PBLs). JfCaspase1 was initially down-regulated but significantly up-regulated at the later stage upon LPS and poly(I:C) challenges in the HKMs. JfCaspase1 was also up-regulated in the Japanese flounder immune-related tissues including head kidney, gill and spleen by bacterial challenge with Edwardsiella tarda. JfCaspase1 protein is comprised of 384 amino acid residues with a calculated molecular mass of 43.75 kDa and is phylogenetically close to fish Caspase1 proteins. JfCaspase1 was co-immunopercipitated with Japanese flounder apoptosis-associated speck-like protein (ASC) when co-expressed in HeLa cells, suggesting that there is a potential interaction between the two proteins. In addition, we showed that extracellular ATP, a potent signaling molecule in activating innate immune response, rapidly up-regulates JfCaspase1 expression and enhances its enzymatic activity both in the HKMs and PBLs. Our findings indicated that the inflammatory JfCaspase1 interacted with ASC protein is implicated in the extracellular ATP-mediated immune signaling in fish. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Radiocesium biokinetics in olive flounder inhabiting the Fukushima accident-affected Pacific coastal waters of eastern Japan.

    Science.gov (United States)

    Tateda, Yutaka; Tsumune, Daisuke; Tsubono, Takaki; Aono, Tatsuo; Kanda, Jota; Ishimaru, Takashi

    2015-09-01

    Radiocesium ((134)Cs and (137)Cs) originating from the Fukushima Dai-ichi Nuclear Power Plant (1FNPP) has contaminated coastal waters and been subsequently transferred to the marine biota along the Pacific coastal region of eastern Japan. To clarify the mechanism of radiocesium biokinetics in olive flounder, a commercially valuable and piscivorous predator, the biokinetics of (137)Cs was simulated using a dynamic biological compartment model and then validated with the measured concentrations in available monitoring data. The (137)Cs concentrations in seawater of the Pacific coastal sites of eastern Japan, from Kesen-numa (170 km north from the 1FNPP) to Choshi (190 km south from the 1FNPP), were reconstructed by fitting the simulated levels to the observed concentrations. Simulated values were verified by measured radiocesium levels in sedentary organism such as macro-algae and mussels inhabiting each study site which had accumulated radiocesium in their ambient environment from the beginning of the accident. Using reconstructed (137)Cs concentrations in seawater, the (137)Cs levels in olive flounder and its main planktivorous prey fish, e.g. anchovy, sand lance, whitebait, etc., were simulated and compared with observed concentrations to clarify the biokinetics of radiocesium in these organisms. This assessment showed that the determining factor for the maximum radiocesium concentrations in fish in the plankton food chain is likely to be the initial radiocesium concentration which they were exposed to during the contamination stage. Furthermore, the simulated (137)Cs concentrations in gut contents of olive flounder were verified by measured (137)Cs concentrations in the stomach contents of this fish collected within 30 km from the 1FNPP. These results indicated that the decrease of (137)Cs levels in their prey organisms was the primary determining factor of radiocesium depuration, and the resultant ecological half-lives were 140-160 d in the olive flounder

  18. Oxidative stress parameters in juvenile Brazilian flounder Paralichthys orbignyanus (Valenciennes, 1839 (Pleuronectiformes: Paralichthyidae exposed to cold and heat shocks

    Directory of Open Access Journals (Sweden)

    Luciano de O. Garcia

    Full Text Available The aim of this study was to determine oxidative stress parameters in the liver and gill of Brazilian flounder juveniles (307.0 ± 16.0 g and 30.0 ± 4.0 cm submitted to different water temperature (17.1, 23.0 and 28.8ºC for 72 h and maintained at salinity 25‰. After the acclimation of 7 days, in 23ºC, fish were transferred to 200 L tanks containing seawater (salinity 25‰ at 28.8ºC (heat shock, 17.1ºC (cold shock or 23.0ºC (control, five replicates (five fish tank-1. The sampled collection occurred in 0 (pre-challenge, 3, 24, 48 and 72 h after temperature shock. Flounder exposed to 17.1ºC and 28.8ºC showed significantly higher TBARS levels and GST activity in the liver post-exposition (PE in relation to the control (23ºC. CAT activity in liver present a significantly increase at 17.1ºC, in first 48 h, and subsequently decrease in 72 h PE in relation to 28.8ºC. The gills of flounder showed significantly higher TBARS levels, GST and CAT activity when submitted at 17.1 and 28.8ºC in relation to 23.0ºC. There were observed changes in lipid peroxidation levels (LPO, CAT and GST activities in the liver and gill of Brazilian flounder in response to reactive oxygen species (ROS produced by thermal shocks.

  19. The Immune Adjuvant Effects of Flounder (Paralichthys olivaceus) Interleukin-6 on E. tarda Subunit Vaccine OmpV.

    Science.gov (United States)

    Guo, Ming; Tang, Xiaoqian; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin

    2017-07-05

    Interleukin-6 (IL-6) as a pleiotropic cytokine was widely used as an effective adjuvant for vaccines in mammals. In this study, the immune adjuvant effects of two forms of flounder (Paralichthys olivaceus) IL-6, including recombinant IL-6 (rIL-6) and pcDNA3.1-IL-6 (pcIL-6), were evaluated and comparatively analyzed on E. tarda subunit vaccine recombinant outer membrane protein V (rOmpV). The results showed that the relative percent survivals of flounder vaccinated with rOmpV plus rIL-6 or pcIL-6 were significantly higher than that in the two control groups, rOmpV plus recombinant 6× histidine-tag (rHis) or empty expression vector pcDNA3.1 (pcN3). The levels of specific serum antibodies and surface membrane immunoglobulin-positive (sIg+) lymphocytes in peripheral blood, spleen, and head kidney in the two adjuvant groups were also much higher than that in the two control groups. Compared with the two control groups, higher upregulated expressions of major histocompatibility complex class Iα (MHCIα), cluster of differentiation 8α (CD8α), MHCIIα, CD4-1, interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were detected in flounder vaccinated with rOmpV plus rIL-6 or pcIL-6 after challenge. In addition, the rOmpV plus rIL-6 could induce significant higher levels of specific serum antibodies, sIg+ lymphocytes and four genes expressions than rOmpV plus pcIL-6. These results demonstrated that both rIL-6 and pcIL-6 used as adjuvants could enhance the immune response and evoke immune protections against E. tarda infection, which has a significant value in controlling diseases using vaccines in flounder.

  20. Comparison of growth characteristics between skeletal muscle satellite cell lines from diploid and triploid olive flounder Paralichthys olivaceus

    Directory of Open Access Journals (Sweden)

    Li-min Peng

    2016-01-01

    Full Text Available Objectives. According to myosatellite cell lines (MSCs established in vitro from diploid and triploid flounder, we compared the characters of growth and differentiation of their MSCs. The results would be useful for learning the muscle development mechanism in teleosts.Materials and Methods. The skeletal muscle cells from the diploid and triploid olive flounder Paralichthys olivaceus were isolated and cultured in vitro, respectively, and the cells were characterized at the morphology and molecular level; meanwhile, the performance of these cells’ proliferation and differentiation were analyzed.Results. Two new skeletal muscle cell lines (POMSCS(2n and POMSCS(3n from diploid and triploid flounder have been respectively subcultured for 67 times and 66 times. The cultured cells were mostly spindle-like mononuclear cells. They have normal flounder diploid karyotype (2n=48t and triploid karyotype (3n=72t, respectively. Muscle satellite cell gene marker (pax7b and myogenic cell protein marker (Desmin were all expressed in cells of two cell lines. Both of the cells could differentiate into the large polynucleated muscle fibre cells, and immunofluorescence reactions of myosin heavy chain (MyHC were positive. There were more cells of POMSCS(3n to differentiate into the muscle fibre cells than that of POMSCS(2n. However, POMSCS(2n cells proliferated more rapidly than those of POMSCS(3n (P < 0.05. The significant fluorescent signals were observed in both POMSCS(2n and POMSCS(3n cells after transfected with pEGFP-N3 reporter plasmid.Conclusions. The two cell lines have been established and characterized as MSCs. We suppose that it might be the differentiation capacity, rather than the proliferation activity of MSCs to play a key role in the better growth of triploid ones than diploid. Both cell lines will become the ideal tools to learn the mechanism of fish MSCs proliferation, differentiation and regeneration during muscle development in the future.

  1. Interaction of carbon tetrachloride with beta-naphthoflavone-mediated cytochrome P450 induction in winter flounder (Pseudopleuronectes americanus).

    Science.gov (United States)

    Kleinow, K M; Droy, B F; Buhler, D R; Williams, D E

    1990-06-15

    The interaction between beta-naphthoflavone induction (BNF: 100 mg/kg) and carbon tetrachloride (CCl4; 1 ml/kg) hepatotoxicity was examined in the flounder. Treatment groups composed of control, BNF, CCl4, and BNF/CCl4 were compared in terms of cytochrome P450 isozyme content (LM4b; LM2), catalytic activity, isozyme distribution. SGOT-SGPT levels, and pathology. CCl4 administration resulted in significant reductions in both the constitutive P450 (LM2) and the BNF-inducible isozyme (LM4b) as well as elevations in SGPT and SGOT levels. The decline in LM4b isozyme content was reflected by stoichiometric decreases in ethoxyresorufin-O-deethylase activities. BNF/CCl4 coadministration was protective in part against CCl4 hepatotoxicity. Immunohistochemistry indicated that LM4b was diffusely distributed throughout the liver. These interactions have demonstrated a multiple P450 isozyme involvement, the protective nature of BNF against CCl4 hepatotoxicity in the flounder, the ability to maintain an inductive response in face of CCl4 coadministration, and the diffuse distributional pattern of LM4b in the flounder liver.

  2. Functional analysis of tumor necrosis factor gene promoter from Japanese flounder, Paralichthys olivaceus, using fish cell lines.

    Science.gov (United States)

    Yazawa, Ryosuke; Hirono, Ikuo; Ohira, Tsuyoshi; Aoki, Takashi

    2005-01-01

    The expression vector pTNF-GFP containing the 2351 bp 5' flanking region of Japanese flounder tumor necrosis factor (TNF) gene was linked with the green fluorescence protein (GFP) gene and was introduced into YO-K cells derived from Japanese flounder kidney and HINAE cells derived Japanese flounder embryos. YO-K cells and HINAE cells were incubated with three concentrations (250, 500, 1000 microg/ml) of lipopolysaccharide (LPS) at 20 degrees C for 24 h. The number of cells expressing GFP, as well as the amount of GFP protein was increased by LPS stimulation in both cell lines. GFP mRNA transcription was also induced by LPS stimulation in both YO-K cells and HINAE cells after 1 h stimulation. In YO-K cells, expression level of GFP decreased gradually from 3 to 6 h post-stimulation, while a reverse trend was observed in HINAE cells. A deletion assay of TNF gene promoter showed that the 5' flanking region, -1783 to -1300 bp, containing cis-acting regulatory elements mediated LPS induction. An electrophoretic mobility shift assay using 2 fragments (-1783 to -1541 bp and -1540 to -1300 bp) revealed that only LPS-stimulated nuclear extracts bound to the -1540 to -1300 bp fragment. These results suggest that transcription of the TNF gene promoter in homologous cultured cells exhibited an inducible pattern and was regulated under the control of the immune system. Copyright 2004 Elsevier Ltd.

  3. Cadmium accumulation and elimination in tissues of juvenile olive flounder, Paralichthys olivaceus after sub-chronic cadmium exposure

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Seong-Gil; Jee, Jung-Hoon; Kang, Ju-Chan

    2004-01-01

    Cd accumulation and elimination in tissue of olive flounder. - Experiments were carried out to investigate the accumulation and elimination of cadmium (Cd) in tissues (gill, intestine, kidney, liver and muscle) of juvenile olive flounder, Paralichthys olivaceus, exposed to sub-chronic concentrations (0, 10, 50, 100 {mu}g l{sup -1}) of Cd. Cd exposure resulted in an increased Cd accumulation in tissues of flounder with exposure periods and concentration, and Cd accumulation in gill and liver increased linearly with the exposure time. At 20 days of Cd exposure, the order of Cd accumulation in organs was gill > intestine > liver > kidney > muscle and after 30 days of exposure, those were intestine > gill > liver > kidney > muscle. An inverse relationship was observed between the accumulation factor (AF) and the exposure level, but AF showed an increase with exposure time. During the depuration periods, Cd concentration in the gill, intestine and liver decreased immediately following the end of the exposure periods. No significant difference was found Cd in concentration in the kidney and muscle during depuration periods. The order of Cd elimination rate in organs were decreased intestine > liver > gill during depuration periods.

  4. Early development of circadian rhythmicity in the suprachiamatic nuclei and pineal gland of teleost, flounder (Paralichthys olivaeus), embryos.

    Science.gov (United States)

    Mogi, Makoto; Uji, Susumu; Yokoi, Hayato; Suzuki, Tohru

    2015-08-01

    Circadian rhythms enable organisms to coordinate multiple physiological processes and behaviors with the earth's rotation. In mammals, the suprachiasmatic nuclei (SCN), the sole master circadian pacemaker, has entrainment mechanisms that set the circadian rhythm to a 24-h cycle with photic signals from retina. In contrast, the zebrafish SCN is not a circadian pacemaker, instead the pineal gland (PG) houses the major circadian oscillator. The SCN of flounder larvae, unlike that of zebrafish, however, expresses per2 with a rhythmicity of daytime/ON and nighttime/OFF. Here, we examined whether the rhythm of per2 expression in the flounder SCN represents the molecular clock. We also examined early development of the circadian rhythmicity in the SCN and PG. Our three major findings were as follows. First, rhythmic per2 expression in the SCN was maintained under 24 h dark (DD) conditions, indicating that a molecular clock exists in the flounder SCN. Second, onset of circadian rhythmicity in the SCN preceded that in the PG. Third, both 24 h light (LL) and DD conditions deeply affected the development of circadian rhythmicity in the SCN and PG. This is the first report dealing with the early development of circadian rhythmicity in the SCN in fish. © 2015 Japanese Society of Developmental Biologists.

  5. Complex movement patterns of greenback flounder (Rhombosolea tapirina) in the Murray River estuary and Coorong, Australia

    Science.gov (United States)

    Earl, Jason; Fowler, Anthony J.; Ye, Qifeng; Dittmann, Sabine

    2017-04-01

    The greenback flounder Rhombosolea tapirina is a commercially-important flatfish species in southern Australia and New Zealand, whose population dynamics are poorly understood. Acoustic telemetry was used to assess movement patterns and area use for R. tapirina in the Murray River estuary and Coorong, South Australia. Twenty fish (221-313 mm total length) equipped with acoustic transmitters were monitored for up to seven months during a period of high freshwater inflow. Fish were detected over a large part of the system, but showed a strong preference for brackish and near-marine conditions in the inner estuary. Tagged fish exhibited complex movement patterns that differed among individuals, including: (1) within estuary movements; (2) dispersal from the estuary to the sea; and (3) return migrations between the estuary and the sea. A diurnal shift in fine-scale area use was observed in the part of the estuary where residency was highest, with individuals occupying deeper habitats during the day and shallower areas during the night. The results demonstrate the individualistic and often highly transient behaviour of this species and its ability to undertake regular movements over the spatial scale of 10s of km. Understanding such movement patterns can improve effective management of estuarine flatfish populations and ecosystems.

  6. Preparation and Characterization of an Olive Flounder (Paralichthys olivaceus) Skin Gelatin and Polylactic Acid Bilayer Film.

    Science.gov (United States)

    Lee, Ka-Yeon; Song, Kyung Bin

    2017-03-01

    Olive flounder skin gelatin (OSG) was used as a film base material. A bilayer film of OSG and polylactic acid (PLA) was prepared using solvent casting method to enhance the film properties. Physical properties of the OSG-PLA film were increased compared with the nonaugmented OSG film. In particular, the PLA lamination decreased water vapor permeability from 2.17 to 0.92 × 10 -9 g·m/m 2 ·s·Pa, as well as of the water solubility from 16.62% to 9.27%, in the bilayer film relative to the OSG film. The oxygen permeability of the OSG-PLA bilayer film was held low by the OSG film, compensating for the high oxygen permeability of the PLA layer. Therefore, the OSG-PLA bilayer film with its enhanced physical properties and high water and oxygen barrier properties can be applied as a food packaging material. © 2017 Institute of Food Technologists®.

  7. A new strain of Cryptocaryon irritans from the cultured olive flounder Paralichthys olivaceus.

    Science.gov (United States)

    Jee, B Y; Kim, K H; Park, S I; Kim, Y C

    2000-12-21

    An obligate parasite, Cryptocaryon irritans, which is responsible for the white spot disease of marine fish is known to develop in the temperature regime over 19 degrees C. Recently, however, we found white spot disease of olive flounder Paralichthys olivaceus during winter at water temperatures ranging between 12 and 16 degrees C in Korea. In the present study we isolated a C. irritans-like ciliate from the affected fish and investigated its reproductive characters to compare the newly found ciliate with typical C. irritans. The newly found ciliate had an additional process in the reproductive stage, characterized by a budding before palintomic division, and it showed a higher ability to carry out tomitogenesis at a low temperature (16 degrees C) than at a high temperature (24 degrees C). Nevertheless, the present ciliates still had much in common with typical C. irritans with respect to clinical, histopathological, and morphological characters, suggesting that it is a new strain of C. irritans, adapted to lower water temperature.

  8. Effects of cooking on levels of PCBs in the fillets of winter flounder (Pseudopleuronectes americanus)

    Energy Technology Data Exchange (ETDEWEB)

    Poston, T.M. [Pacific Northwest Lab., Richland, WA (United States); Durell, G.S.; Koczwara, G.; Spellacy, A.M. [Battelle Ocean Sciences, Duxbury, MA (United States)

    1995-08-01

    The Pacific Northwest Laboratory and Battelle Ocean Sciences performed a study to determine the effect of cooking on polychlorinated biphenyl (PCB) levels in the fillets of winter flounder (Pseudopleuronectes americanus). Broiling, pan frying, and deep frying in oil were tested on fillets from 21 fish collected from New Bedford Harbor, Massachusetts, on February 21, 1991. The evaluation involved estimating the change in PCB concentrations using a mass-balance approach that factored the change in fillet weight resulting from cooking with the changes in PCB concentration expressed on a precooked wet-weight basis. Deep frying in oil resulted in a 47% reduction in total PCB levels in fillet tissue. Additionally, deep frying caused a 40% reduction in fillet mass. Pan frying and broiling resulted in statistically in insignificant increases in total PCB levels of 15% and 17%, respectively. Fillet mass reductions resulting from pan frying and broiling were 7% and 15%, respectively. The effects of cooking on 18 individual congeners generally paralleled the results observed for total PCB. All 18 congeners were significantly reduced by deep frying. Congener Cl{sub 2}(08) also was significantly reduced by either pan frying. Congeners Cl{sub 5}(105) and Cl{sub 5}(118) showed apparent significant increases in concentrations following pan frying. Congeners Cl{sub 5}(105), Cl{sub 5}(118), and C1{sub 6}(138) showed significant increases in concentration following broiling.

  9. Involvement of melanin-concentrating hormone 2 in background color adaptation of barfin flounder Verasper moseri.

    Science.gov (United States)

    Mizusawa, Kanta; Kawashima, Yusuke; Sunuma, Toshikazu; Hamamoto, Akie; Kobayashi, Yuki; Kodera, Yoshio; Saito, Yumiko; Takahashi, Akiyoshi

    2015-04-01

    In teleosts, melanin-concentrating hormone (MCH) plays a key role in skin color changes. MCH is released into general circulation from the neurohypophysis, which causes pigment aggregation in the skin chromatophores. Recently, a novel MCH (MCH2) precursor gene, which is orthologous to the mammalian MCH precursor gene, has been identified in some teleosts using genomic data mining. The physiological function of MCH2 remains unclear. In the present study, we cloned the cDNA for MCH2 from barfin flounder, Verasper moseri. The putative prepro-MCH2 contains 25 amino acids of MCH2 peptide region. Liquid chromatography-electrospray ionization mass spectrometry with a high resolution mass analyzer were used for confirming the amino acid sequences of MCH1 and MCH2 peptides from the pituitary extract. In vitro synthesized MCH1 and MCH2 induced pigment aggregation in a dose-dependent manner. A mammalian cell-based assay indicated that both MCH1 and MCH2 functionally interacted with both the MCH receptor types 1 and 2. Mch1 and mch2 are exclusively expressed in the brain and pituitary. The levels of brain mch2 transcript were three times higher in the fish that were chronically acclimated to a white background than those acclimated to a black background. These results suggest that in V. moseri, MCH1 and MCH2 are involved in the response to changes in background colors, during the process of chromatophore control. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. Purification and determination of two novel antioxidant peptides from flounder fish (Paralichthys olivaceus) using digestive proteases.

    Science.gov (United States)

    Ko, Ju-Young; Lee, Ji-Hyeok; Samarakoon, Kalpa; Kim, Jin-Soo; Jeon, You-Jin

    2013-02-01

    We investigated the effects of bioactive-peptides from hydrolysates of flounder fish muscle (FFM) on antioxidant activity. The hydrolysates were prepared by enzymatic reactions of FFM using eight commercial proteases such as papain, pepsin, trypsin, neutrase, alcalase, kojizyme, protamex, and α-chymotrypsin. The α-chymotrypsin hydrolysate showed the strongest antioxidant activity among the eight enzymatic hydrolysates. Further separation of the α-chymotrypsin hydrolysate was performed by ultrafiltration, gel filtration, and reverse-phase high performance liquid chromatography. Consequently, two novel peptides with high antioxidant activity were purified, and their amino acid sequences were determined (Val-Cys-Ser-Val [VCSV] and Cys-Ala-Ala-Pro [CAAP], respectively). The two peptides showed good scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical (IC(50) values, 111.32 and 26.89 μM, respectively) and high cytoprotective activities against 2,2-azobis-(2-amidino-propane) dihydrochloride (AAPH) without cytotoxicity and scavenged total reactive oxygen species in Vero cells. In particular, apoptotic bodies produced by AAPH dose-dependently decreased following treatment with the CAAP peptide. These results revealed firstly the two peptides with strong antioxidative effects from FFM. Copyright © 2012 Elsevier Ltd. All rights reserved.

  11. Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus

    Science.gov (United States)

    Peng, Limin; Zheng, Yuan; You, Feng; Wu, Zhihao; Zou, Yuxia; Zhang, Peijun

    2016-09-01

    The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical "S" shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.

  12. Evolution history of duplicated smad3 genes in teleost: insights from Japanese flounder, Paralichthys olivaceus.

    Science.gov (United States)

    Du, Xinxin; Liu, Yuezhong; Liu, Jinxiang; Zhang, Quanqi; Wang, Xubo

    2016-01-01

    Following the two rounds of whole-genome duplication (WGD) during deuterosome evolution, a third genome duplication occurred in the ray-fined fish lineage and is considered to be responsible for the teleost-specific lineage diversification and regulation mechanisms. As a receptor-regulated SMAD (R-SMAD), the function of SMAD3 was widely studied in mammals. However, limited information of its role or putative paralogs is available in ray-finned fishes. In this study, two SMAD3 paralogs were first identified in the transcriptome and genome of Japanese flounder (Paralichthys olivaceus). We also explored SMAD3 duplication in other selected species. Following identification, genomic structure, phylogenetic reconstruction, and synteny analyses performed by MrBayes and online bioinformatic tools confirmed that smad3a/3b most likely originated from the teleost-specific WGD. Additionally, selection pressure analysis and expression pattern of the two genes performed by PAML and quantitative real-time PCR (qRT-PCR) revealed evidence of subfunctionalization of the two SMAD3 paralogs in teleost. Our results indicate that two SMAD3 genes originate from teleost-specific WGD, remain transcriptionally active, and may have likely undergone subfunctionalization. This study provides novel insights to the evolution fates of smad3a/3b and draws attentions to future function analysis of SMAD3 gene family.

  13. Immunoglobulin Tau Heavy Chain (IgT in Flounder, Paralichthys olivaceus: Molecular Cloning, Characterization, and Expression Analyses

    Directory of Open Access Journals (Sweden)

    Yang Du

    2016-09-01

    Full Text Available Immunoglobulin tau (IgT is a new teleost immunoglobulin isotype, and its potential function in adaptive immunity is not very clear. In the present study, the membrane-bound and secreted IgT (mIgT and sIgT heavy chain genes were cloned for the first time and characterized in flounder (Paralichthys olivaceus, and found the nucleic acid sequence were exactly same in the Cτ1–Cτ4 constant domains of mIgT and sIgT, but different in variable regions and the C-terminus. The amino acid sequence of mIgT shared higher similarity with Bovichtus diacanthus (51.2% and Dicentrarchus labrax (45.0%. Amino acid of flounder IgT, IgM, and IgD heavy chain was compared and the highest similarity was found between IgT Cτ1 and IgM Cμ1 (38%. In healthy flounder, the transcript levels of IgT mRNA were the highest in gill, spleen, and liver, and higher in peripheral blood leucocytes, skin, and hindgut. After infection and vaccination with Edwardsiella tarda via intraperitoneal injection and immersion, the qRT-PCR analysis demonstrated that the IgT mRNA level was significantly upregulated in all tested tissues, with similar dynamic tendency that increased firstly and then decreased, and higher in gill, skin, hindgut, liver, and stomach in immersion than in the injection group, but no significant difference existed in spleen and head kidney between immersion and injection groups. These results revealed that IgT responses could be simultaneously induced in both mucosal and systemic tissues after infection/vaccination via injection and immersion route, but IgT might play a more important role in mucosal immunity than in systemic immunity.

  14. Determination of ghrelin structure in the barfin flounder (Verasper moseri and involvement of ingested fatty acids in ghrelin acylation

    Directory of Open Access Journals (Sweden)

    Hiroyuki eKaiya

    2013-09-01

    Full Text Available Ghrelin is a peptide hormone that is acylated with a fatty acid, usually n-octanoic acid, at the third amino acid residue (usually a serine or threonine, and this acylation is known to be essential for ghrelin activity not only in mammals but also in non-mammals, such as fish. However, the modification mechanisms of ghrelin modification in fish are not known. In this study, we elucidated the structure of ghrelin in a teleost, the barfin flounder (Verasper moseri, and determined whether ingested free fatty acids of various chain lengths participated in ghrelin acylation. Complementary DNA cloning revealed the barfin flounder prepro-ghrelin to be a 106-amino acid (aa peptide and the mature ghrelin to be a 20-aa peptide (GSSFLSPSHKPPNKGKPPRA. However, purification of ghrelin peptides from stomach extracts demonstrated that the major form of the hormone was a 19-aa decanoylated peptide (GSS[C10:0]FLSPSHKPPNKGKPPR missing the last alanine of the 20-aa peptide. Ingestion of feed enriched with n-heptanoic acid (C7, n-octanoic acid (C8, or n-nonanoic acid (C9 changed the modification status of the peptide: ingestion of C8 or C9 increased the amount of C8:0 or C9:0 19-aa ghrelin, respectively, but no C7:0 ghrelin was isolated after ingestion of C7. These results indicate that ingested free fatty acids are substrates for ghrelin acylation in the barfin flounder, but the types of free fatty acids utilized as substrates may be limited.

  15. An integrated assessment of pollution and biological effects in flounder, mussels and sediment in the southern Baltic Sea coastal area.

    Science.gov (United States)

    Dabrowska, Henryka; Kopko, Orest; Lehtonen, Kari K; Lang, Thomas; Waszak, Ilona; Balode, Maija; Strode, Evita

    2017-02-01

    Organic and metal contaminants and biological effects were investigated in flounder, mussels, and sediments in the southern Baltic Sea coastal area in order to assess environmental quality status in that area. Four sites were selected, including two within the Gulf of Gdańsk (GoG). In biota and sediment at each site, DDTs dominated over PCBs and PBDEs were the least abundant among organic contaminants. Their concentrations decreased progressively outward from GoG. Among metal contaminants, the levels of Hg, Pb, and Cd were elevated in GoG. Biomarkers in flounder, EROD activity and DNA SB, showed moderate positive correlations with organic and metal contaminants. In flounder, the integrated biomarker index (IBR/n) presented a spatial trend coherent with chemical pollution index (CPI), but there was no clear spatial correspondence between IBR/n and CPI in mussels nor between sediment toxicity index (STI) and sediment CPI. The integrated assessment of contaminant and biological effect data against available assessment criteria indicated that in biota, the contaminant assessment thresholds were most often exceeded by CB-118, heptachlor, PBDE, and Hg (in the GoG sediments by p,p'-DDT, Hg and Cd), while of the biological determinants, the threshold was breeched by AChE activity in mussels in GoG. Applying the ICES/OSPAR traffic-light approach showed that of the 50 parameters assessed at each site, there were 18% of determinants in the red color category in the two GoG sites and 8% of determinants in the two sites outside GoG, which indicated that none of the four investigated sites attained good environmental status (GES).

  16. Mercury in flounder, Platichtys flesus, cod, Gadus morhua, and perch, Perca fluviatilis, in relation to their length and environment

    Energy Technology Data Exchange (ETDEWEB)

    Luten, J.B.; Bouquet, W.; Riekwel-Booy, G.; Rauchbaar, A.B.; Scholte, M.W.M.

    1987-02-01

    During the last ten years several changes have been taken place in the use of mercury. Industrial discharges have been restricted and several fields of application have been eliminated or reduced. However, the use of mercury containing batteries is still increasing. Monitoring the mercury content of fish is however still an important aspect of environmental pollution control. It has been shown by several investigators that the mercury content in fish might be correlated with biological parameters like length and age. In this paper the results are presented of a few recent Dutch monitoring studies of mercury in flounder, cod and perch in relation to biological and environmental parameters.

  17. Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences

    Science.gov (United States)

    Xu, Dongdong; Li, Sanlei; Lou, Bao; Zhang, Yurong; Zhan, Wei; Shi, Huilai

    2012-07-01

    Japanese flounder is one of the most important commercial species in China; however, information on the genetic background of natural populations in China seas is scarce. The lack of genetic data has hampered fishery management and aquaculture development programs for this species. In the present study, we have analyzed the genetic diversity in natural populations of Japanese flounder sampled from the Yellow Sea (Qingdao population, QD) and East China Sea (Zhoushan population, ZS) using 10 polymorphic microsatellite loci and cytochrome c oxidase subunit I (COI) sequencing data. A total of 68 different alleles were observed over 10 microsatellite loci. The total number of alleles per locus ranged from 2 to 9, and the number of genotypes per locus ranged from 3 to 45. The observed heterozygosity and expected heterozygosity in QD were 0.733 and 0.779, respectively, and in ZS the heterozygosity values were 0.708 and 0.783, respectively. Significant departures from Hardy-Weinberg equilibrium were observed in 7 of the 10 microsatellite loci in each of the two populations. The COI sequencing analysis revealed 25 polymorphic sites and 15 haplotypes in the two populations. The haplotype diversity and nucleotide diversity in the QD population were 0.746±0.072 8 and 0.003 34±0.001 03 respectively, and in ZS population the genetic diversity values were 0.712±0.047 0 and 0.003 18±0.000 49, respectively. The microsatellite data ( F st =0.048 7, P <0.001) and mitochondrial DNA data ( F st =0.128, P <0.001) both revealed significant genetic differentiation between the two populations. The information on the genetic variation and differentiation in Japanese flounder obtained in this study could be used to set up suitable guidelines for the management and conservation of this species, as well as for managing artificial selection programs. In future studies, more geographically diverse stocks should be used to obtain a deeper understanding of the population structure of Japanese

  18. Effects of modified atmosphere packaging on toxin production by Clostridium botulinum in raw aquacultured summer flounder fillets (Paralichthys dentatus).

    Science.gov (United States)

    Arritt, Fletcher M; Eifert, Joseph D; Jahncke, Michael L; Pierson, Merle D; Williams, Robert C

    2007-05-01

    Packaging fishery products under vacuum atmosphere packaging (VAC) and modified atmosphere packaging (MAP) conditions can significantly extend the shelf life of raw, refrigerated fish products. There is considerable commercial interest in marketing VAC and MAP refrigerated (never frozen) raw fish fillets. The objective of this study was to determine if Clostridium botulinum toxin development precedes microbiological spoilage in raw, refrigerated flounder fillets. Aquacultured flounder (Paralichthys dentatus) individual fish fillets either were packed with a film having an oxygen transmission rate (OTR) of 3000 cm3 m(-2) 24 h(-1) at 22.8 degrees C or were vacuum packaged or packaged under 100% CO2 with a film having an OTR of 7.8 cm3 m(-2) 24 h(-1) at 21.1 degrees C and were stored at 4 and 10 degrees C. Samples were analyzed by aerobic plate count (APC) for spoilage and qualitatively for botulinum toxin with a mouse bioassay. The results demonstrate that flounder fillets (4 degrees C) packaged with a film having an OTR of 3,000 were microbiologically spoiled (APC, > 10(7) CFU/g) on day 15, but there was no toxin formation, even after 35 days of storage. However, at 10 degrees C, toxin production occurred (day 8), but it was after microbial spoilage and absolute sensory rejection (day 5). Vacuum-packaged fillets and 100% CO2 fillets (4 degrees C) packaged with a film having an OTR of 7.8 were toxic on days 20 and 25, respectively, with microbial spoilage (APC, >10(7) CFU/g) not occurring during the tested storage period (i.e., >35 days). At 10 degrees C, in vacuum-packaged flounder, toxin formation coincided with microbiological spoilage (days 8 to 9). In the 100% CO2-packaged fillets, toxin formation occurred on day 9, with microbial spoilage occurring on day 15. This study indicates that films with an OTR of 3,000 can be used for refrigerated fish fillets and still maintain the safety of the product.

  19. Design and operation of seawater recirculation fish culture system for Japanese flounder; Hirameyo junkan roka yogyo system no sekkei to un`yo

    Energy Technology Data Exchange (ETDEWEB)

    Honda, H.; Kikuchi, K.; Watanabe, Y.; Iwata, N.; Takeda, S.; Uemoto, H.; Furuta, T.; Kiyono, M. [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1994-08-01

    For developing a recirculation fish culture system for Japanese flounder, a pilot system of 20m{sup 3} of water was designed and constructed, and a verification test was conducted. The system comprises an FRP-made breeding tank, 6m in internal diameter and 1.15m in height, a nitrification tank, a pump, a heat pump using air as the heat source, a UV germicidal unit, a denitrification tank, etc. The verification test revealed that flounder seeds the average body weight 5g grew up to a marketable weight of 500g in approximately 10 months. Thanks to the water purifying nitrification tank the concentration of ammonia and nitrous acid was kept at not more than 1mg per liter. The denitrification tank also functioned satisfactorily for the removal of nitric acid from the breeding water. The quantity of seawater per flounder produced was 1/2000 of the quantity required by the conventional method wherein seawater continuously flows. A tentative calculation of the running cost indicates that, with 12 of the system of this design in use, a total cost of 1000 to 1100 yen per year was required, the said total cost covering the seeds, feed, and electric power, for the production of 1kg of flounder, which sum is approximately equal to what is needed in the conventional method. 36 refs., 7 figs., 3 tabs.

  20. Cloning and stage-specific expression of CK-M1 gene during metamorphosis of Japanese flounder, Paralichthys olivaceus

    Science.gov (United States)

    Chen, Yanjie; Zhang, Quanqi; Qi, Jie; Wang, Zhigang; Wang, Xubo; Sun, Yeying; Zhong, Qiwang; Li, Shuo; Li, Chunmei

    2010-05-01

    The symmetrical body of flatfish larvae changes dramatically into an asymmetrical form after metamorphosis. The molecular mechanisms responsible for this change are poorly understood. As an initial step to clarify these mechanisms, we used representational difference analysis of cDNA for the identification of genes active during metamorphosis in the Japanese flounder, Paralichthys olicaceus. One of the up-regulated genes was identified as creatine kinase muscle type 1 (CK-M1). Sequence analysis of CK-M1 revealed that it spanned 1 708 bp and encoded a protein of 382 amino acids. The overall amino acid sequence of the CK-M1 was highly conserved with those of other organisms. CK-M1 was expressed in adult fish tissues, including skeletal muscle, intestine and gill. Whole mount in-situ hybridization showed that the enhanced expression of CK-M1 expanded from the head to the whole body of larvae as metamorphosis progressed. Quantitative analysis revealed stage-specific high expression of CK-M1 during metamorphosis. The expression level of CK-M1 increased initially and peaked at metamorphosis, decreased afterward, and finally returned to the pre-metamorphosis level. This stage-specific expression pattern suggested strongly that CK-M1 was related to metamorphosis in the Japanese flounder. Its specific role in metamorphosis requires further study.

  1. Disruption of the thyroid system by the thyroid-disrupting compound Aroclor 1254 in juvenile Japanese flounder (Paralichthys olivaceus.

    Directory of Open Access Journals (Sweden)

    Yifei Dong

    Full Text Available Polychlorinated biphenyls (PCBs are a group of persistent organochlorine compounds that have the potential to disrupt the homeostasis of thyroid hormones (THs in fish, particularly juveniles. In this study, thyroid histology, plasma TH levels, and iodothyronine deiodinase (IDs, including ID1, ID2, and ID3 gene expression patterns were examined in juvenile Japanese flounder (Paralichthys olivaceus following 25- and 50-day waterborne exposure to environmentally relevant concentrations of a commercial PCB mixture, Aroclor 1254 (10, 100, and 1000 ng/L with two-thirds of the test solutions renewed daily. The results showed that exposure to Aroclor 1254 for 50 d increased follicular cell height, colloid depletion, and hyperplasia. In particular, hypothyroidism, which was induced by the administration of 1000 ng/L Aroclor 1254, significantly decreased plasma TT4, TT3, and FT3 levels. Profiles of the changes in mRNA expression levels of IDs were observed in the liver and kidney after 25 and 50 d PCB exposure, which might be associated with a reduction in plasma THs levels. The expression level of ID2 mRNA in the liver exhibited a dose-dependent increase, indicating that this ID isotype might serve as sensitive and stable indicator for thyroid-disrupting chemical (TDC exposure. Overall, our study confirmed that environmentally relevant concentrations of Aroclor 1254 cause significant thyroid disruption, with juvenile Japanese flounder being suitable candidates for use in TDC studies.

  2. Trends in nitrogen isotope ratios of juvenile winter flounder reflect changing nitrogen inputs to Rhode Island, USA estuarine systems.

    Science.gov (United States)

    Pruell, Richard J; Taplin, Bryan K; Miller, Kenneth M

    2017-05-15

    Nitrogen isotope ratios (δ15N) in juvenile winter flounder, Pseudopleuronectes americanus, were used to examine changes in nitrogen inputs to several Rhode Island, USA estuarine systems. Fish were collected over two three-year periods with a ten-year interval between sampling periods (2002-2004 and 2012-2014). During that interval numerous changes to nutrient management practices were initiated in the watersheds of these estuarine systems including the upgrade of several major wastewater treatment facilities that discharge to Narragansett Bay, which significantly reduced nitrogen inputs. Following these reductions, the δ15N values of flounder in several of the systems decreased as expected; however, isotope ratios in fish from upper Narragansett Bay significantly increased. We believe that low δ15N values measured in 2002-2004 were related to concentration-dependent fractionation at this location. Increased δ15N values measured between 2012 and 2014 may indicate reduced fractionation or that changes in wastewater treatment processes altered the nitrogen isotopic ratios of the effluents. Published by Elsevier Ltd.

  3. Molecular characterization, origin, and evolution of teleost p68 gene family: Insights from Japanese flounder, Paralichthys olivaceus.

    Science.gov (United States)

    Wang, Zhongkai; Liu, Wei; Zhou, Nayu; Wang, Huizhen; Li, Peizhen; Wang, Mengxun; Zhang, Quanqi

    2015-12-01

    Two rounds of whole-genome duplication occurred in the common ancestor of vertebrates. Later, a third round genome duplication occurred in the teleost fishes. As a prototype member of DEAD-box RNA helicases, the function of p68 helicase in development has been well investigated in human, however, limited information is available regarding the regulatory function of this gene in the development of teleosts. In this study, being an important farmed fish in North China, Japanese flounder (Paralichthys olivaceus) was used as model fish to investigate the role of p68 gene in teleost development. Two p68 genes were first identified from Japanese flounder. Molecular characterization of them was performed by analyzing the exon-intron boundaries. Then, we confirmed that such two teleost p68 genes originated from teleost-specific genome duplication through phylogenetic and synteny analyses. Additionally, comparative analyses of amino acid sequences, variation in selective pressure, and expression profiles of p68 genes revealed probable sub-functionalization fate of teleost p68 genes after the duplication. Therefore, this study supplements the evolutionary properties of teleost p68 gene family and provides the groundwork for further studying the regulatory function of p68 genes in the development of teleosts. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

  4. Interferon regulatory factor 3 (IRF-3) in Japanese flounder, Paralichthys olivaceus: sequencing, limited tissue distribution, inducible expression and induction of fish type I interferon promoter.

    Science.gov (United States)

    Hu, Guobin; Yin, Xiangyan; Lou, Huimin; Xia, Jun; Dong, Xianzhi; Zhang, Jianyie; Liu, Qiuming

    2011-02-01

    Two cDNAs with different 3'-untranslated region (UTR) encoding an interferon regulatory factor 3 (IRF-3) were cloned from head kidney of Japanese flounder, Paralichthys olivaceus, by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. Sequence analysis reveals that they were generated by alternative polyadenylation. The predicted protein consists of 467 amino acid residues which shares the highest identity of 50.7-57.6% to fish IRF-3 and possesses a DNA-binding domain (DBD), an IRF association domain (IAD) and a serine-rich domain (SRD) of vertebrate IRF-3. The presence of these domains along with phylogenetic analysis places it into the IRF-3 group of the IRF-3 subfamily. RT-PCR analysis revealed that flounder IRF-3 was expressed constitutively in limited tissue types including head kidney, spleen, kidney, heart, gill, intestine and liver. A quantitative real time PCR assay was employed to monitor expression of IRF-3, type I interferon (IFN) and Mx in flounder head kidney and gill. All three genes were up-regulated by polyinosinic:polycytidylic acid (polyI:C) and lymphocystis disease virus (LCDV) with an earlier but slight and less persistent increase in transcription levels seen for the IRF-3. Finally, flounder IRF-3 was proved to induce fish type I IFN promoter in FG9307 cells, a flounder gill cell line, by a luciferase assay. These results provide insights into the roles of fish IRF-3 in the antiviral immunity. Copyright © 2010 Elsevier Ltd. All rights reserved.

  5. Commercial fishing gear modifications to reduce interactions between Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) and the southern flounder (Paralichthys lethostigma) fishery in North Carolina (USA).

    Science.gov (United States)

    Levesque, Juan C; Hager, Christian; Diaddorio, Eric; Dickey, R Jason

    2016-01-01

    Bycatch of protected species in commercial fishing operations is a primary concern to fishery managers because it threatens the conservation, protection, and recovery of fragile species, such as the Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus). One potential solution to reduce the risk associated with commercial fishing operations is to design commercial fishing gear that is more selective in terms of interactions between Atlantic sturgeon and commercial fisheries. Given this conservation and management need, the overarching goal was to reduce Atlantic sturgeon fishery interactions and maintain southern flounder (Paralichthys lethostigma) catch in North Carolina. The specific objectives of this study were to design and evaluate the effectiveness of a modified gillnet. Overall, the results proved that lowering the profile and amount of webbing had a beneficial impact at reducing Atlantic sturgeon incidental encounters and bycatch. The modified gillnet reduced bycatch and Atlantic sturgeon encounters by 39.6% and 60.9%, respectively. Our design entangled 51.6% fewer southern flounder, which corresponded to a 48.9% reduction in total weight; the modified gear entangled slightly larger southern flounder than the control gear. Our findings showed the number of Atlantic sturgeon encounters was positively associated with mean water depth, with more Atlantic sturgeon encountered in deeper (5.1-6.3 m) than shallower waters; 75% were encountered at depths between 4.6 and 6.1 m. Most southern flounder (n = 518, 39.7%) were taken at a water depth between 3.76 and 5.0 m. This observation suggests that southern flounder prefer slightly shallower waters than Atlantic sturgeon.

  6. Criopreservación de espermatozoides del lenguado Paralichthys adspersus Cryopreservation of flounder Paralichthys adspersus spermatozoa

    Directory of Open Access Journals (Sweden)

    Christian Catcoparco

    2012-07-01

    Full Text Available Para optimizar las técnicas de reproducción en cautiverio de lenguado Paralichthys adspersus; se elaboró una metodología para la criopreservación de sus espermatozoides. Para ello, se evaluó el efecto del dimetil sulfóxido (DMSO como agente crioprotector en tres concentraciones diferentes (1,0; 1,5 y 2,0 M sobre la motilidad espermática y su posterior congelación con cinco diferentes tasas: -7,5; -10; -12,5; -20 y -30°C min-1, utilizando un congelador automático programable. Los mayores porcentajes de motilidad espermática post congelamiento-descongelamiento (40,5 ± 13% fueron obtenidos al utilizar una tasa de -10°C min-1 y DMSO sin encontrar diferencias significativas entre las tres diferentes concentraciones de DMSO (P To optimize the techniques of captive breeding of flounder Paralichthys adspersus, a methodology was developed for the cryopreservation of spermatozoa of this species. The effect on sperm motility post-thawing, using three different concentrations (1.0; 1.5 and 2.0 M of DMSO as cryoprotective agent and five different freezing rates -7.5; -10; -12.5; -20 and -30°C min-1, with an automatic programmable freezer was evaluated. The highest percentages of post-thawing sperm motility were obtained by freezing sperm at -10°C min-1, no significant differences (P < 0.05 were founded between the three different concentrations of DMSO used. Subsequently, we evaluated the effect of a non-permeable cryo-additive (chicken egg yolk, VHG, in order to obtain an increasing of the percentage of sperm motility. We used a cryoprotectant solution including DMSO at three different concentrations adding 10% VHG v/v. The highest percentages of sperm motility (71.71 ± 13% were obtained at the freezing rate of -10°C min-1 without significant differences between the three concentrations of the cryoprotectant solution in which the sample was incubated sperm (P < 0.05. A high significant difference between the sperm motility percentages post

  7. European communion

    DEFF Research Database (Denmark)

    Manners, Ian James

    2013-01-01

    Political theory of European union, through an engagement between political concepts and theoretical understandings, provides a means of identifying the EU as a political object. It is argued that understanding the projects, processes and products of European union, based on ‘sharing’ or ‘communion......’, provides a better means of perceiving the EU as a political object rather than terms such as ‘integration’ or ‘co-operation’. The concept of ‘European communion’ is defined as the ‘subjective sharing of relationships’, understood as the extent to which individuals or groups believe themselves to be sharing...... relations (or not), and the consequences of these beliefs for European political projects, processes and products. By exploring European communion through an engagement with contemporary political theory, using very brief illustrations from the Treaty of Lisbon, the article also suggests that European...

  8. Integrated monitoring of chemicals and their effects on four sentinel species, Limanda limanda, Platichthys flesus, Nucella lapillus and Mytilus sp., in Seine Bay: A key step towards applying biological effects to monitoring.

    Science.gov (United States)

    Burgeot, Thierry; Akcha, Farida; Ménard, Dominique; Robinson, Craig; Loizeau, Véronique; Brach-Papa, Christophe; Martínez-Gòmez, Concepción; Le Goff, Jérémie; Budzinski, Hélène; Le Menach, Karine; Cachot, Jérome; Minier, Christophe; Broeg, Katja; Hylland, Ketil

    2017-03-01

    The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological assessment thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the Comet assay, DNA adducts, micronucleus (MN), PAH metabolites, imposex, intersex and fish external pathologies were analysed in four marine sentinel species (Platichthys flesus, Limanda limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and heavy metals were analysed in biota and sediment. Results for sediment and four species in 2008-2009 made it possible to quantify the impact of contaminants using thresholds (Environmental Assessment Criteria/EAC2008: 70% and EAC2009: 60%) and effects (EAC2008: 50% and EAC2009: 40%) in the Seine estuary. The Seine estuary is ranked among Europe's most highly polluted sites. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. A multiple endpoint analysis of the effects of chronic exposure to sediment contaminated with Deepwater Horizon oil on juvenile Southern flounder and their associated microbiomes

    Energy Technology Data Exchange (ETDEWEB)

    Brown-Peterson, Nancy J., E-mail: nancy.brown-peterson@usm.edu [Department of Coastal Sciences, The University of Southern Mississippi, 703 East Beach Dr., Ocean Springs, MS 39564 (United States); Krasnec, Michelle, E-mail: MKrasnec@stratusconsulting.com [Abt Associates, 1881 Ninth Street, Suite 201, Boulder, Colorado 80302 (United States); Takeshita, Ryan, E-mail: RTakeshita@stratusconsulting.com [Abt Associates, 1881 Ninth Street, Suite 201, Boulder, Colorado 80302 (United States); Ryan, Caitlin N., E-mail: Caitlin.ryan@ttu.edu [The Institute of Environmental and Human Health, Department of Environmental Toxicology, Texas Tech University, Box 41163, Lubbock, TX 79409 (United States); Griffitt, Kimberly J., E-mail: kim.griffitt@gmail.com [Department of Coastal Sciences, The University of Southern Mississippi, 703 East Beach Dr., Ocean Springs, MS 39564 (United States); Lay, Claire, E-mail: claymsc@stratusconsulting.com [Abt Associates, 1881 Ninth Street, Suite 201, Boulder, Colorado 80302 (United States); Mayer, Gregory D., E-mail: greg.mayer@ttu.edu [The Institute of Environmental and Human Health, Department of Environmental Toxicology, Texas Tech University, Box 41163, Lubbock, TX 79409 (United States); Bayha, Keith M., E-mail: kmbayha@gmail.com [Department of Coastal Sciences, The University of Southern Mississippi, 703 East Beach Dr., Ocean Springs, MS 39564 (United States); Hawkins, William E., E-mail: william.hawkins@usm.edu [Department of Coastal Sciences, The University of Southern Mississippi, 703 East Beach Dr., Ocean Springs, MS 39564 (United States); Lipton, Ian, E-mail: ianlipton@stratusconsulting.com [Abt Associates, 1881 Ninth Street, Suite 201, Boulder, Colorado 80302 (United States); Morris, Jeffrey, E-mail: JMorrisMSCanyon252@stratusconsulting.com [Abt Associates, 1881 Ninth Street, Suite 201, Boulder, Colorado 80302 (United States); and others

    2015-08-15

    Highlights: • Juvenile southern flounder were exposed to sediment mixed with different amount of oil from the Deepwater Horizon spill. • The exposure was performed for 32 days, with growth and survival assessed throughout. • After the termination of the experiment, the survivors were examined at multiple endpoints, including histopathology and microbiome analysis. • The results indicated that the flounder were adversely affected at each endpoint examined. • Growth and survival were significantly reduced. • Histopathology found effects on gills and livers of exposed fish. • The microbiomes of the exposed fish were significantly altered by the exposure to sediment-associated oil in both gills and intestines. - Abstract: Exposure to oiled sediments can negatively impact the health of fish species. Here, we examine the effects of chronic exposure of juvenile southern flounder, Paralichthys lethostigma, to a sediment-oil mixture. Oil:sediment mixtures are persistent over time and can become bioavailable following sediment perturbation or resuspension. Juvenile flounder were exposed for 32 days under controlled laboratory conditions to five concentrations of naturally weathered Macondo MC252 oil mixed into uncontaminated, field-collected sediments. The percent composition of individual polycyclic aromatic hydrocarbons (PAHs) of the weathered oil did not change after mixing with the sediment. Spiked exposure sediments contained 0.04–395 mg/kg tPAH50 (sum of 50 individual PAH concentration measurements). Mortality increased with both exposure duration and concentration of sediment-associated PAHs, and flounder exposed to concentrations above 8 mg/kg tPAH50 showed significantly reduced growth over the course of the experiment. Evident histopathologic changes were observed in liver and gill tissues of fish exposed to more than 8 mg/kg tPAH50. All fish at these concentrations showed hepatic intravascular congestion, macrovesicular hepatic vacoulation

  10. Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus.

    Science.gov (United States)

    Zheng, Y; Peng, L M; You, F; Zou, Y X; Zhang, P J; Chen, S L

    2015-07-01

    A new brain-cell line derived from Japanese flounder Paralichthys olivaceus (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid-like. Chromosome analysis revealed the cell line to possess the normal P. olivaceus diploid karyotype of 2n = 48t (telocentric chromosomes). The cells exhibited the astrocyte marker glial fibrillary acidic protein by immunocytochemistry, and significant fluorescent signals were observed when the cells were transfected with green fluorescent protein reporter plasmid. The established POBC would be ideal material for the study of function of fish ependyma, the central neuroendocrine system and endocrine disruptors in the marine environment. © 2015 The Fisheries Society of the British Isles.

  11. Up-regulation of hepatic receptor for growth hormone in the flounder ( Paralichthys olivaceus) after oral administration with exogenous GH

    Science.gov (United States)

    Liu, Zong-Zhu; Wang, Jin-Bao; Xu, Yong-Li; Wang, Yong; Zhang, Pei-Jun

    2001-06-01

    The iodination efficiency of salmon GH(sGH) was 38.82%, using a modification of the chloramine-T method. The specific activity of the125I-sGH was about 40 μCi/μg protein. The results of binding assay showed a single class of high affinity and low-capacity binding site in flounder liver. Long-term administration with exogenous GH can induce the up-regulation of hepatic GH receptor in total binding capacity though there was no significant difference in capacity of free binding sites of livers from control and experimental fish, this result also indicated that the liver from experimental fish, compared to that from control fish, had more occupied binding sites.

  12. European Institutions?

    NARCIS (Netherlands)

    Meacham, Darian

    2016-01-01

    The aim of this article is to sketch a phenomenological theory of political institutions and to apply it to some objections and questions raised by Pierre Manent about the project of the European Union and more specifically the question of “European Construction”, i.e. what is the aim of the

  13. Selective Europeanization

    DEFF Research Database (Denmark)

    Hoch Jovanovic, Tamara; Lynggaard, Kennet

    2014-01-01

    political contexts at the European level. We further show how the “translation” of international norms to a domestic context has worked to reinforce the original institutional setup, dating back to the mid-1950s. The translation of European-level minority policy developed in the 1990s and 2000s works most...

  14. Effects of extruded pellet and moist pellet on growth performance, body composition, and hematology of juvenile olive flounder, Paralichthys olivaceus

    Directory of Open Access Journals (Sweden)

    Seunghan Lee

    2016-10-01

    Full Text Available Abstract A feeding trial was conducted to evaluate the effects of two different sizes of extruded pellets (EP (EP1 - 3 mm or EP2 - 5 mm and a moist pellet (MP in olive flounder, Paralichthys olivaceus, reared in semi-recirculation system. A total of 450 fish with an average initial weight of 5.0 ± 0.2 g (mean ± SD were fed one of the three experimental diets in triplicate groups. At the end of a 6-week feeding trial, weight gain, specific growth rate, and feed efficiency of fish fed EP diets were significantly higher than those of fish fed MP (P < 0.05. Water quality parameters like turbidity, total ammonia nitrogen, and total phosphorous from tanks of fish fed EP1 and EP2 were significantly lower than those from tanks of fish fed MP. Blood plasma glutamic oxaloacetic transaminase and glucose concentration were significantly higher in fish fed MP diet compared to fish fed EP diets (P < 0.05. Whole body crude protein contents in fish fed EP diets were higher than those from the fish fed MP diet. Whole body amino acid content like threonine, aspartic acid, serine, tyrosine, and cystine were found to be significantly higher in fish fed EP diets than those in fish fed MP diet. In considering overall performance of olive flounder, EP2 diet could be recommended for the successful aquaculture of this important fish species.

  15. Effect of water temperature on survival of early-life stages of marbled flounder Pseudopleuronectes yokohamae in Tokyo Bay, Japan.

    Science.gov (United States)

    Lee, Jeong-Hoon; Kodama, Keita; Oyama, Masaaki; Shiraishi, Hiroaki; Horiguchi, Toshihiro

    2017-07-01

    We investigated factors that might have disturbed the stock recovery of marbled flounder in Tokyo Bay by focusing on the early life stages. Field surveys in Tokyo Bay from 2006 to 2011 revealed that mature adult biomass increased from 2006 to 2008 and decreased thereafter. Meanwhile, larval and juvenile densities were high in 2006 and 2008 but low in other years. Discrepancies in the yearly trends of these parameters suggest that mortality during life stages between spawning and early larval phases might have affected the abundance of the subsequent life stages. Monthly mean water temperature between January and February, in which hatching and pelagic larvae occur in the bay, was lower in 2006 (8.6 °C) and 2008 (9.6 °C) than was observed in other years (10.4-11.4 °C). Significant negative correlation between water temperature and larval density implies that mortality during pre- and post-larval stages would be higher in warmer winter years (>10 °C). To test this hypothesis, we examined the effects of water temperature on mortality and development in egg and larval stages under controlled laboratory conditions. Hatching rate was high in a water temperature range of 9.2-12.7 °C (66.6-82.5%), whereas it decreased in cooler (3.7% at 5.9 °C) or warmer (33.9% at 14.8 °C) conditions. Meanwhile, days from fertilization to hatching, size of larvae at hatching and survival rate of larvae after 18 d from hatching were monotonically and significantly decreased as water temperature was elevated. Combined evidence of the field and laboratory studies suggests that a warmer reproductive season (>10 °C) might induce mortalities of marbled flounder larvae in Tokyo Bay. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Distribution and expression in vitro and in vivo of DNA vaccine against lymphocystis disease virus in Japanese flounder ( Paralichthys olivaceus)

    Science.gov (United States)

    Zheng, Fengrong; Sun, Xiuqin; Liu, Hongzhan; Wu, Xingan; Zhong, Nan; Wang, Bo; Zhou, Guodong

    2010-01-01

    Lymphocystis disease, caused by the lymphocystis disease virus (LCDV), is a significant worldwide problem in fish industry causing substantial economic losses. In this study, we aimed to develop the DNA vaccine against LCDV, using DNA vaccination technology. We evaluated plasmid pEGFP-N2-LCDV1.3 kb as a DNA vaccine candidate. The plasmid DNA was transiently expressed after liposome transfection into the eukaryotic COS 7 cell line. The distribution and expression of the DNA vaccine (pEGFP-N2-LCDV1.3kb) were also analyzed in tissues of the vaccinated Japanese flounder by PCR, RT-PCR and fluorescent microscopy. Results from PCR analysis indicated that the vaccine-containing plasmids were distributed in injected muscle, the muscle opposite the injection site, the hind intestine, gill, spleen, head, kidney and liver, 6 and 25 days after vaccination. The vaccine plasmids disappeared 100 d post-vaccination. Fluorescent microscopy revealed green fluorescence in the injected muscle, the muscle opposite the injection site, the hind intestine, gill, spleen, head, kidney and liver of fish 48 h post-vaccination, green fluorescence did not appear in the control treated tissue. Green fluorescence became weak at 60 days post-vaccination. RT-PCR analysis indicated that the mcp gene was expressed in all tested tissues of vaccinated fish 6-50 days post-vaccination. These results demonstrate that the antigen encoded by the DNA vaccine is distributed and expressed in all of the tissues analyzed in the vaccinated fish. The antigen would therefore potentially initiate a specific immune response. the plasmid DNA was injected into Japanese flounder ( Paralichthys olivaceus) intramuscularly and antibodies against LCDV were evaluated. The results indicate that the plasmid encoded DNA vaccine could induce an immune response to LCDV and would therefore offer immune protection against LCD. Further studies are required for the development and application of this promising DNA vaccine.

  17. Microarray analysis of hepatic gene expression in juvenile Japanese flounder Paralichthys olivaceus fed diets supplemented with fish or vegetable oils.

    Science.gov (United States)

    Limtipsuntorn, Ubonrat; Haga, Yutaka; Kondo, Hidehiro; Hirono, Ikuo; Satoh, Shuichi

    2014-02-01

    Gene expression profiling was performed in Japanese flounder Paralichthys olivaceus fed diets supplemented with fish oil (FO), linseed oil (LO), or olive oil (OO) for 6 weeks. The LO and OO groups showed significantly retarded growth, lower feed intake, lower protein efficiency ratio, and lower hepatosomatic index (P < 0.05). Liver fatty acid composition reflected the dietary fatty acid composition. Microarray analysis revealed that dietary n - 3 highly unsaturated fatty acid (HUFA) deficiency affected 169 transcripts. In the LO group, 57 genes were up-regulated and 38 genes were down-regulated, whereas in the OO group nine genes were up-regulated and 87 genes were down-regulated. Analysis of the functional annotations suggested that dietary n - 3 HUFA affected genes involved in signal transduction (23.2 %), cellular processes (21.1 %), metabolism (including glucose, lipid, and nucleobase; 15.5 %), transport (11.3 %), regulation of transcription (10.5 %), and immune response (4.2 %). Several genes encoding serine/threonine kinases such as protein kinase C and calmodulin-dependent kinase and nuclear hormone receptors such as vitamin D receptor, retinoic acid receptor, and receptors for cytokines (bone morphogenic protein and transforming growth factor β) were affected. Among 169 transcripts, 22 genes were affected in both LO and OO groups. The present study identified several genes involved in n - 3 HUFA deficiency-sensitive pathways, which will be useful for selective breeding of flounder strains able to adapt to n - 3 HUFA deficiency.

  18. Expression of cytokine genes in head kidney and spleen cells of Japanese flounder (Paralichthys olivaceus) infected with Nocardia seriolae.

    Science.gov (United States)

    Tanekhy, M; Matsuda, S; Itano, T; Kawakami, H; Kono, T; Sakai, M

    2010-04-15

    Nocardiosis caused by Nocardia seriolae is an important disease affecting marine fish for which neither control nor preventive measures are available. In this study, we investigated cytokine gene expression in Japanese flounder (Paralichthys olivaceus) infected with N. seriolae to understand the innate immune response. Japanese flounder were challenged with different concentrations of N. seriolae suspensions (0, 1, and 10 mg/L) by immersion for 10min. Mortality was 75% and 95% in fish infected by 1 and 10 mg/L, respectively. The expression of cytokine genes (TNF-alpha, IL-1beta, CC-chemokine) in head kidney and spleen cells to N. seriolae challenge was investigated 2, 24 h, 3 days, and 10 days post-challenge. TNF-alpha expression was significantly increased in spleen after 24 h in 1 mg/L group and in HK after 2 h in 10 mg/L group, but after 24 h and 3 days in 10 mg/L group and after 3 days in 1 mg/L group, it was significantly decreased. IL-1beta expression was significantly up-regulated in spleen after 24 h in 1 mg/l group while in HK only after 2 h in 10 mg/L group before suddenly down-regulated significantly 24 h in 10 mg/L group. The expression of CC-chemokine gene in both spleen and HK was significantly up-regulated in 10 mg/L group 2 h post-challenge and down-regulated in HK after 24 h and after 10 days in 1 mg/L group in spleen, compared to the control group. Copyright 2009 Elsevier B.V. All rights reserved.

  19. Chronic effects of light irradiated from LED on the growth performance and endocrine properties of barfin flounder Verasper moseri.

    Science.gov (United States)

    Takahashi, Akiyoshi; Kasagi, Satoshi; Murakami, Naoto; Furufuji, Sumihisa; Kikuchi, Shigeto; Mizusawa, Kanta; Andoh, Tadashi

    2016-06-01

    We investigated the effects of specific wavelengths of light on the growth of barfin flounder. The fish, reared in white tanks in a dark room, were irradiated with light from light-emitting diodes (LEDs) with peak wavelengths of 464nm (blue), 518nm (green), and 635nm (red) under a controlled photoperiod (10.5:13.5, light-dark cycle; 06:00-16:30, light). Fish were reared for four weeks in three independent experiments at three different water temperatures (averages of 14.9°C, 8.6°C, and 6.6°C). The fish irradiated with blue and green light had higher specific growth rates (% body weight⋅day(-1)) than fish irradiated with red light. Notably, green light had the greatest effect on growth among the three light wavelengths at 6.6°C. In the brains of fish reared at 6.6°C, the amounts of melanin-concentrating hormone 1 mRNA under green light were lower than those under red light, and amounts of proopiomelanocortin-C mRNA under blue and green light were higher than those under red light. No differences were observed for other neuropeptides tested. In the pituitary, no difference was observed in growth hormone mRNA content. In plasma, higher levels of insulin and insulin-like growth factor-I were observed in fish under green light than those of fish under red light. These results suggest that the endocrine systems of barfin flounder are modulated by a specific wavelength of light that stimulates somatic growth. Copyright © 2016 Elsevier Inc. All rights reserved.

  20. Dynamic responses of prolactin, growth hormone and their receptors to hyposmotic acclimation in the olive flounder Paralichthys olivaceus.

    Science.gov (United States)

    Yuan, Mingzhe; Jia, Qianqian; Wang, Ting; Lu, Qi; Tang, Langlang; Wang, Youji; Lu, Weiqun

    2017-12-01

    Prolactin (PRL) and growth hormone (GH) play important roles in regulating salt and water balance through osmoregulatory organs in vertebrates. The aim of this study was to investigate the dynamic changes of GH/PRL hormone gene expressions in the pituitary gland and their receptors in gill and kidney, as well as the plasma osmolality when the olive flounder fish Paralichthys olivaceus were acclimated in freshwater (FW) conditions. After transfer from seawater (SW) to freshwater (FW), the osmolality of FW-adaption fish reached the lowest level at 1d which rose slightly afterwards. However, the hormone gene expression of PRL increased from 2d, reaching its peak at 5d, and then decreased at 14d. At this time, the value was still significantly higher than the control, showing a similar trend to the plasma hormone PRL. In contrast, the pituitary mRNA level of GH significantly decreased at 1d and then returned to normal levels. The mRNA levels of PRL receptor (PRLR) in both gill and kidney displayed a similar trend to the pituitary PRL. We also observed the synchronous expression trend of the renal PRLR with pituitary PRL (5d) and the asynchronous expression peaks between branchial (8d) and renal PRLR (5d). Significant responses of GH and its receptor (GHR) in both gill and kidney during the FW-acclimation were not observed. Nevertheless, the gene expression of GH receptor variant (GHR-V) in both gill and kidney declined at 2d, indicating unknown osmoregulatory functions of GHR-V. Collectively, our results provided more insights of the PRL, GH and their corresponding receptors in modulating osmoregulatory responses, representing an important aspect of FW-acclimation in flounder fish. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. European Whiteness?

    DEFF Research Database (Denmark)

    Blaagaard, Bolette

    2008-01-01

    Born out of the United States’ (U.S.) history of slavery and segregation and intertwined with gender studies and feminism, the field of critical whiteness studies does not fit easily into a European setting and the particular historical context that entails. In order for a field of European...... critical whiteness studies to emerge, its relation to the U.S. theoretical framework, as well as the particularities of the European context need to be taken into account.. The article makes a call for a multi-layered approach to take over from the identity politics so often employed in the fields of U...

  2. Characterization of CD3(+) T lymphocytes of Japanese flounder (Paralichthys olivaceus) and its response after immunization with formalin-inactivated Edwardsiella tarda.

    Science.gov (United States)

    Tang, Xiaoqian; Qin, Yinghui; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin

    2017-04-01

    The CD3 complex is an important cell surface marker of T lymphocytes and essential for T lymphocytes activation in higher vertebrates. In the present work, the CD3ε of Japanese flounder (Paralichthys olivaceus) was recombinantly expressed in E. coli BL21 (DE3) and used as an immunogen to produce mouse anti-rCD3ε polyclonal antibodies, which could specifically recognize a 20 kDa protein in the membrane proteins of peripheral blood lymphocytes (PBL) of Japanese flounder by co-immunoprecipitation assay. Mass spectrometric analysis showed the 20 kDa protein was the native CD3ε of Japanese flounder. Both the flow cytometric analysis and double immunofluorescence assay (DIFA) showed that the CD3(+) T lymphocytes could be identified specifically by the mouse anti-rCD3ε polyclonal antibodies, which didn't cross-react with the sIgM(+) lymphocytes. Immunohistochemistry showed that CD3(+) T lymphocytes could be detected in gill, skin, stomach, intestine, spleen, liver, head-kidney and mid-kidney. Flow cytometric analysis showed the percentages of CD3(+) T lymphocytes in the PBL, spleen lymphocytes (SL) and head-kidney lymphocytes (HKL) of Japanese flounder increased rapidly after immunization with formalin-inactivated Edwardsiella tarda, and reached their peak levels at 5th day with 12.6%, 9.7% and 8.7%, respectively, and then decreased gradually. These results suggested that CD3(+) T lymphocytes play important roles in mucosal and cell-mediated immunity, and the results would deepen our understanding on the roles of teleost T lymphocytes in the immune response. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Poly (I:C) and imiquimod induced immune responses and their effects on the survival of olive flounder (Paralichthys olivaceus) from viral haemorrhagic septicaemia.

    Science.gov (United States)

    Avunje, Satheesha; Jung, Sung-Ju

    2017-10-18

    The stimulation of immune genes by polyinosinic:polycytidylic acid (poly (I:C)) and imiquimod in olive flounder (Paralichthys olivaceus) and their role in control of viral haemorrhagic septicaemia virus (VHSV) infection were examined. Poly (I:C) (100 μg/fish) treated olive flounder had very low mortality (5%) post VHSV infection, while the imiquimod treated group had 65% and 85% mortality at a dose of 100 μg/fish and 50 μg/fish, respectively. Though the imiquimod treated group had high mortality, it was lower than the untreated group, which had 90% mortality. In vivo experiments were conducted to determine effect of the two ligands on immune modulation in the head kidney of olive flounder. Poly (I:C) activated the immune genes (TLR-3, TLR-7, MDA-5, LGP-2, IRF-3, IRF-7, IL-1β type I IFN and Mx) very early, within 1 d post stimulation, faster and stronger than imiquimod. Though Mx levels were enhanced by imiquimod, the host was still susceptible to VHSV. The poly (I:C) treated group had a high immune response at the time of infection and 1 dpi, though it decreased at later stages. The imiquimod treated group and the unstimulated group had a higher immune response to VHSV compared to the poly (I:C) treated group. The nucleoprotein copies of VHSV were very low in the poly (I:C) treated group but interestingly, were high in both untreated and imiquimod treated fish. Thus, host survival from a viral infection does not only depend on the quantity of immune response but also the time of response. Although imiquimod enhanced immune gene expression in olive flounder, a delayed response could be the reason for high mortality to VHS compared with poly (I:C), which induced the immune system effectively and efficiently to protect the host. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Molecular cloning, expression pattern, and 3D structural prediction of the cold inducible RNA-binding protein (CIRP) in Japanese flounder ( Paralichthys olivaceus)

    Science.gov (United States)

    Yang, Xiao; Gao, Jinning; Ma, Liman; Li, Zan; Wang, Wenji; Wang, Zhongkai; Yu, Haiyang; Qi, Jie; Wang, Xubo; Wang, Zhigang; Zhang, Quanqi

    2015-02-01

    Cold-inducible RNA-binding protein (CIRP) is a kind of RNA binding proteins that plays important roles in many physiological processes. The CIRP has been widely studied in mammals and amphibians since it was first cloned from mammals. On the contrary, there are little reports in teleosts. In this study, the Po CIRP gene of the Japanese flounder was cloned and sequenced. The genomic sequence consists of seven exons and six introns. The putative PoCIRP protein of flounder was 198 amino acid residues long containing the RNA recognition motif (RRM). Phylogenetic analysis showed that the flounder PoCIRP is highly conserved with other teleost CIRPs. The 5' flanking sequence was cloned by genome walking and many transcription factor binding sites were identified. There is a CpGs region located in promoter and exon I region and the methylation state is low. Quantitative real-time PCR analysis uncovered that Po CIRP gene was widely expressed in adult tissues with the highest expression level in the ovary. The mRNA of the Po CIRP was maternally deposited and the expression level of the gene was regulated up during the gastrula and neurula stages. In order to gain the information how the protein interacts with mRNA, we performed the modeling of the 3D structure of the flounder PoCIRP. The results showed a cleft existing the surface of the molecular. Taken together, the results indicate that the CIRP is a multifunctional molecular in teleosts and the findings about the structure provide valuable information for understanding the basis of this protein's function.

  5. Changes of cytochrome P4501A mRNA expression and physiology responses in the olive flounder, Paralichthys olivaceus, exposed to benzo(a)pyrene

    Energy Technology Data Exchange (ETDEWEB)

    Choi, C.Y.; An, K.W.; Shin, H.S.; An, M.I.; Jo, P.G. [Korean Maritime University, Pusan (Republic of Korea). Division of Marine Environmental and Bioscience

    2008-07-01

    Benzo(a)pyrene (BaP) is generated by the incomplete combustion of organic substances such as oil and coal, and is a widespread organic environmental contaminant in terrestrial and aquatic ecosystems. To determine the effects of BaP on organisms, we investigated its time- and dose-related effects on the levels of cytochrome P4501A (P4501A) mRNA in the liver and gills of the olive flounder (Paralichthys olivaceus) using quantitative polymerase chain reaction (QPCR) and measured the plasma glucose, cortisol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels. The full-length olive flounder P4501A cDNA consists of 1566 nucleotides and encodes a 521-amino-acid protein. In the liver and gills, the expression of P4501A mRNA was highest 6 h after exposure to both 10 and 30 gl{sup -1} BaP, and then decreased. In addition, the plasma parameters increased with exposure. These results suggest that P4501A plays an important role in the detoxification of BaP, which stressed the olive flounder. Therefore, these physiological parameters may be indicators of BaP-induced stress responses.

  6. Ink from longfin inshore squid, Doryteuthis pealeii, as a chemical and visual defense against two predatory fishes, summer flounder, Paralichthys dentatus, and sea catfish, Ariopsis felis.

    Science.gov (United States)

    Derby, Charles D; Tottempudi, Mihika; Love-Chezem, Tiffany; Wolfe, Lanna S

    2013-12-01

    Chemical and visual defenses are used by many organisms to avoid being approached or eaten by predators. An example is inking molluscs-including gastropods such as sea hares and cephalopods such as squid, cuttlefish, and octopus-which release a colored ink upon approach or attack. Previous work showed that ink can protect molluscs through a combination of chemical, visual, and other effects. In this study, we examined the effects of ink from longfin inshore squid, Doryteuthis pealeii, on the behavior of two species of predatory fishes, summer flounder, Paralichthys dentatus, and sea catfish, Ariopsis felis. Using a cloud assay, we found that ink from longfin inshore squid affected the approach phase of predation by summer flounder, primarily through its visual effects. Using a food assay, we found that the ink affected the consummatory and ingestive phase of predation of both sea catfish and summer flounder, through the ink's chemical properties. Fractionation of ink showed that most of its deterrent chemical activity is associated with melanin granules, suggesting that either compounds adhering to these granules or melanin itself are the most biologically active. This work provides the basis for a comparative approach to identify deterrent molecules from inking cephalopods and to examine neural mechanisms whereby these chemicals affect behavior of fish, using the sea catfish as a chemosensory model.

  7. European Security

    DEFF Research Database (Denmark)

    Møller, Bjørn

    Theoretical chapters on "Security", "Organisations" and "Regions," Historical Chapters on "Europe and Its Distinguishing Features" and on "The United Nations," "NATO," "The CSCE/OSCE and the Council of Europe" and "The European Union"......Theoretical chapters on "Security", "Organisations" and "Regions," Historical Chapters on "Europe and Its Distinguishing Features" and on "The United Nations," "NATO," "The CSCE/OSCE and the Council of Europe" and "The European Union"...

  8. pHj, contractility and Ca-balance under hypercapnic acidosis in the myocardium of different vertebrate species

    DEFF Research Database (Denmark)

    Gesser, H; Bonefeld-Jørgensen, Eva Cecilie

    1982-01-01

    The influence of hypercapnic acidosis upon the heart was examined in four vertebrate species. The CO2 in the tissue bath was increased from 2.7 to 15% at 12 degrees C for flounder (Platichthys flesus) and cod (Gadus morhua) and from 3 to 13% at 22 degrees C for turtle (Pseudemys scripta) and rain......The influence of hypercapnic acidosis upon the heart was examined in four vertebrate species. The CO2 in the tissue bath was increased from 2.7 to 15% at 12 degrees C for flounder (Platichthys flesus) and cod (Gadus morhua) and from 3 to 13% at 22 degrees C for turtle (Pseudemys scripta...

  9. European visit

    CERN Multimedia

    2006-01-01

    The European Commissioner for Science and Research, Janez Potočnik, (on the right) visited the CMS assembly hall accompanied by Jim Virdee, Deputy Spokesman of CMS (on the left), and Robert Aymar, Director-General of CERN. The European Commissioner for Science and Research, Janez Potočnik, visited CERN on Tuesday 31 January. He was welcomed by the Director-General, Robert Aymar, who described the missions and current activities of CERN to him, in particular the realisation of the LHC with its three components: accelerator, detectors, storage and processing of data. The European Commissioner then visited the CMS assembly hall, then the hall for testing the LHC magnets and the ATLAS cavern. During this first visit since his appointment at the end of 2004, Janez Potočnik appeared very interested by the operation of CERN, an example of successful scientific co-operation on a European scale. The many projects (30 on average) that CERN and the European Commission carry out jointly for the benefit of res...

  10. European Cinema

    NARCIS (Netherlands)

    Elsaesser, Thomas

    2005-01-01

    In the face of renewed competition from Hollywood since the early 1980s and the challenges posed to Europe's national cinemas by the fall of the Wall in 1989, independent filmmaking in Europe has begun to re-invent itself. European Cinema: Face to Face with Hollywood re-assesses the different

  11. Establishment and characterization of a new marine fish cell line from ovary of barfin flounder ( Verasper moseri)

    Science.gov (United States)

    Xu, Xiaohui; Fan, Tingjun; Jiang, Guojian; Yang, Xiuxia

    2015-12-01

    A novel continuous ovary cell line from barfin flounder ( Verasper moseri) (BFO cell line) was established with its primitive application in transgenic expression demonstrated in this study. Primarily cultured cells grew well at 22°C in Dulbecco's modified Eagle medium/F12 medium (DMEM/F12, 1:1; pH 7.2) supplemented with 20% fetal bovine serum (FBS), carboxymethyl chitooligosaccharide, basic fibroblast growth factor (bFGF) and insulin-like growth factor-I (IGF-I). The primary BFO cells in fibroblastic morphology proliferated into a confluent monolayer about 2 weeks later, and were able to be subcultured. Impacts of medium and temperature on the growth of the cells were examined. The optimum growth was found in DMEM/F12 with 20% FBS and at 22°C. The BFO cells can be continuously subcultured to Passage 120 steadily with a population doubling time of 32.7 h at Passage 60. Chromosome analysis revealed that 72% of BFO cells at Passage 60 maintained the normal diploid chromosome number (46) with a normal karyotype of 2st+44t. The results of gene transformation indicated that green fluorescence protein (GFP) positively expressed in these cells after being transformed with pcDNA3.1-GFP. Therefore, a continuous and transformable BFO cell line was successfully established, which may serve as a useful tool for cytotechnological manipulation and transgenic modification of this fish.

  12. Interferon-induced protein 56 (IFI56) is induced by VHSV infection but not by bacterial infection in olive flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Hwang, Jee Youn; Ahn, Sang Jung; Kwon, Mun-Gyeong; Seo, Jung Soo; Hwang, Seong Don; Son, Maeng-Hyun

    2017-07-01

    Interferon-inducible protein 56 (IFI56, also known as ISG56/IFIT1, interferon-induced protein with tetratricopeptide repeats 1) is strongly induced in response to interferon and a potent inhibitor of viral replication and translational initiation. Here, we describe the identification of IFI56 (OfIFI56) in olive flounder, its characteristic features, and expression levels in various tissues before and after viral hemorrhagic septicemia virus (VHSV) infection. The full-length OfIFI56 sequence was identified from rapid amplification of cDNA ends PCR. The complete coding sequence of OfIFI56 is 1971 bp in length and encodes 431 amino acids. The putative OfIFI56 protein has multiple tetratricopeptide (TPR) motifs, which regulate diverse biological processes, such as organelle targeting, protein import, and vesicle fusion. Based on sequence analysis, the Larimichthys crocea IFI56 protein (61%) had the highest sequence homology to OfIFI56. In healthy olive flounder, OfIFI56 mRNA expression was detected in many tissues such as intestine, gill, head kidney, heart, spleen, and trunk kidney tissues. After VHSV challenge, OfIFI56 mRNA was significantly up-regulated in these tissues. Additionally, OfIFI56 expression was induced by poly I:C but not by Streptococcus parauberis and S. iniae infection or lipopolysaccharide injection in kidney and spleen tissues of olive flounder. These results demonstrate that piscine OfIFI56 expression is not induced by bacterial infection but is selectively induced by viral infection, especially VHSV, and that OfIFI56 may play an important role in the host response against VHSV infection. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Live vaccine of viral hemorrhagic septicemia virus (VHSV) for Japanese flounder at fish rearing temperature of 21°C instead of Poly(I:C) administration.

    Science.gov (United States)

    Nishizawa, Toyohiko; Takami, Ikuo; Yang, Minji; Oh, Myung-Joo

    2011-10-26

    The process of "Poly(I:C) immunization" involves immunization of fish with a pathogenic live virus, followed by administration of Poly(I:C), which induces a transient, non-specific antiviral state. As a result, fish in an antiviral state survive the initial immunization with live virus. Moreover, these fish are able to mount a specific protective immune response against the injected pathogenic virus. In the present study, we investigated the optimum temperature for Poly(I:C) immunization of Japanese flounder Paralichthys olivaceus with live viral hemorrhagic septicemia virus (VHSV). It was revealed that the optimum temperature was around at 17°C for Poly(I:C) immunization in Japanese flounder. Furthermore, the protection efficacy of Poly(I:C) immunization was significantly decreased by elevation of fish rearing temperature, and no efficacy was observed at a fish rearing temperature of 25°C. Interestingly, no mortality by VHSV infection was observed in fish reared at 21°C and 25°C even when those fish were not administered Poly(I:C). All of the survivors from the first VHSV-challenge at 21°C were strongly protected from re-challenge with VHSV. However, almost all of the survivors (≥82.6%) from the first challenge at 25°C were lost by the second challenge with VHSV. It was thus concluded that by rearing fish at 21°C and challenging with live VHSV, it is possible to induce strong specific immunity in Japanese flounder without Poly(I:C) administration. Copyright © 2011 Elsevier Ltd. All rights reserved.

  14. Identification and functional analysis of dual-specificity MAP kinase phosphatase 6 gene (dusp6) in response to immune challenges in Japanese flounder Paralichthys olivaceus.

    Science.gov (United States)

    Li, Shuo; Peng, Weijiao; Hao, Gaixiang; Li, Jiafang; Geng, Xuyun; Sun, Jinsheng

    2017-01-01

    Dual-specificity phosphatase 6 (Dusp6) is a member of mitogen-activated protein kinase (MAPK) phosphatases that play crucial roles in regulating MAPK signaling and immune response. The immunological relevance of Dusp6 in fish, however, remains largely uncharacterized. In the present study, a full-length Japanese flounder dusp6 cDNA ortholog, termed PoDusp6, was identified and characterized from Paralichthys olivaceus. The deduced PoDusp6 protein is comprised of 383 amino acids with a conserved N-terminal regulatory rhodanese homology domain and a C-terminal catalytic domain. Immunofluorescence microscopy revealed that PoDusp6 protein is mainly localized in cytoplasm. Sequence analysis indicates that PoDusp6 is highly conserved (>70% identity) throughout the evolution from teleost to mammals. In unstimulated conditions, PoDusp6 mRNA was present in all examined tissues and showed the highest expression in Japanese flounder head kidney macrophages (HKMs). Immune challenge experiments revealed that the expression of PoDusp6 was down-regulated at the early stage after LPS and poly(I:C) stimulations but significantly up-regulated at the later stage in the HKMs. The similar expression pattern was also observed in the Japanese flounder immune-related tissues including head kidney, gill and spleen upon bacterial challenge with Edwardsiella tarda. Overexpression of PoDusp6 in Japanese flounder FG-9307 cells led to a significant down-regulation of proinflammatory cytokine genes IL-1beta, TNF-alpha and IFN-gamma, and antiviral gene Mx. Interestingly, inhibition of Dusp6 activity also down-regulated the LPS-induced IL-beta gene expression but did not affected on the LPS-induced IFN-gamma and TNF-alpha expression in the HKMs. Our findings suggest that the expression of PoDusp6 is modulated by immune stimuli and PoDusp6 may act as an essential modulator in fish inflammatory response. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. Catabolic signaling pathways, atrogenes, and ubiquitinated proteins are regulated by the nutritional status in the muscle of the fine flounder.

    Science.gov (United States)

    Fuentes, Eduardo N; Ruiz, Pamela; Valdes, Juan Antonio; Molina, Alfredo

    2012-01-01

    A description of the intracellular mechanisms that modulate skeletal muscle atrophy in early vertebrates is still lacking. In this context, we used the fine flounder, a unique and intriguing fish model, which exhibits remarkably slow growth due to low production of muscle-derived IGF-I, a key growth factor that has been widely acknowledged to prevent and revert muscle atrophy. Key components of the atrophy system were examined in this species using a detailed time-course of sampling points, including two contrasting nutritional periods. Under basal conditions high amounts of the atrogenes MuRF-1 and Atrogin-1 were observed. During fasting, the activation of the P38/MAPK and Akt/FoxO signaling pathways decreased; whereas, the activation of the IκBα/NFκB pathway increased. These changes in signal transduction activation were concomitant with a strong increase in MuRF-1, Atrogin-1, and protein ubiquitination. During short-term refeeding, the P38/MAPK and Akt/FoxO signaling pathways were strongly activated, whereas the activation of the IκBα/NFκB pathway decreased significantly. The expression of both atrogenes, as well as the ubiquitination of proteins, dropped significantly during the first hour of refeeding, indicating a strong anti-atrophic condition during the onset of refeeding. During long-term refeeding, Akt remained activated at higher than basal levels until the end of refeeding, and Atrogin-1 expression remained significantly lower during this period. This study shows that the components of the atrophy system in skeletal muscle appeared early in the evolution of vertebrates and some mechanisms have been conserved, whereas others have not. These results represent an important achievement for the area of fish muscle physiology, showing an integrative view of the atrophy system in a non-mammalian species and contributing to novel insights on the molecular basis of muscle growth regulation in earlier vertebrates.

  16. Catabolic signaling pathways, atrogenes, and ubiquitinated proteins are regulated by the nutritional status in the muscle of the fine flounder.

    Directory of Open Access Journals (Sweden)

    Eduardo N Fuentes

    Full Text Available A description of the intracellular mechanisms that modulate skeletal muscle atrophy in early vertebrates is still lacking. In this context, we used the fine flounder, a unique and intriguing fish model, which exhibits remarkably slow growth due to low production of muscle-derived IGF-I, a key growth factor that has been widely acknowledged to prevent and revert muscle atrophy. Key components of the atrophy system were examined in this species using a detailed time-course of sampling points, including two contrasting nutritional periods. Under basal conditions high amounts of the atrogenes MuRF-1 and Atrogin-1 were observed. During fasting, the activation of the P38/MAPK and Akt/FoxO signaling pathways decreased; whereas, the activation of the IκBα/NFκB pathway increased. These changes in signal transduction activation were concomitant with a strong increase in MuRF-1, Atrogin-1, and protein ubiquitination. During short-term refeeding, the P38/MAPK and Akt/FoxO signaling pathways were strongly activated, whereas the activation of the IκBα/NFκB pathway decreased significantly. The expression of both atrogenes, as well as the ubiquitination of proteins, dropped significantly during the first hour of refeeding, indicating a strong anti-atrophic condition during the onset of refeeding. During long-term refeeding, Akt remained activated at higher than basal levels until the end of refeeding, and Atrogin-1 expression remained significantly lower during this period. This study shows that the components of the atrophy system in skeletal muscle appeared early in the evolution of vertebrates and some mechanisms have been conserved, whereas others have not. These results represent an important achievement for the area of fish muscle physiology, showing an integrative view of the atrophy system in a non-mammalian species and contributing to novel insights on the molecular basis of muscle growth regulation in earlier vertebrates.

  17. Studies on substitutional protein sources for fish meal in the diet of Japanese flounder; Hirame shiryo ni okeru miriyo shigen no riyo

    Energy Technology Data Exchange (ETDEWEB)

    Kikuchi, K.; Furuta, T.; Sakaguchi, I. [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1996-08-01

    Effectiveness of livestock industry wastes and vegetable protein added to fish meal in fish farming is tested by feeding the Japanese flounder. In the experiment, a part or the whole of the fish meal protein is replaced by the meat meal (MM), meat and bone meal (MBM), corngluten meal (CGM), or dried silkworm pupa meal (SPM), and fries of the Japanese flounder are fed on the new diets for eight weeks. On a diet containing 60% or less of MM, no change is detected in the fish in terms of increase in weight, protein efficiency ratio, and blood components, indicating that 60% at the highest of fish meal may be replaced by MM. In the case of MBM, it can occupy approximately 20%. As for CGM, the proper substitution rate is approximately 40%. Essential amino acids that the new diets may lack are added for an approximately 10% improvement on the result. The SPM substitution works up to 40%, when, however, the blood components are degraded. The proper substitution rate is therefore placed at approximately 20%. 38 refs., 2 figs., 17 tabs.

  18. Cloning of the cDNA encoding adenosine 5'-monophosphate deaminase 1 and its mRNA expression in Japanese flounder Paralichthys olivaceus

    Science.gov (United States)

    Jiang, Keyong; Sun, Shujuan; Liu, Mei; Wang, Baojie; Meng, Xiaolin; Wang, Lei

    2013-01-01

    AMP deaminase catalyzes the conversion of AMP into IMP and ammonia. In the present study, a full-length cDNA of AMPD1 from skeletal muscle of Japanese flounder Paralichthys olivaceus was cloned and characterized. The 2 526 bp cDNA contains a 5'-UTR of 78 bp, a 3'-UTR of 237 bp and an open reading frame (ORF) of 2 211 bp, which encodes a protein of 736 amino acids. The predicted protein contains a highly conserved AMP deaminase motif (SLSTDDP) and an ATP-binding site sequence (EPLMEEYAIAAQVFK). Phylogenetic analysis showed that the AMPD1 and AMPD3 genes originate from the same branch, but are evolutionarily distant from the AMPD2 gene. RT-PCR showed that the flounder AMPD1 gene was expressed only in skeletal muscle. QRT-PCR analysis revealed a statistically significant 2.54 fold higher level of AMPD1 mRNA in adult muscle (750±40 g) compared with juvenile muscle (7.5±2 g) ( Panimals.

  19. Changes in microRNAs expression profile of olive flounder (Paralichthys olivaceus) in response to viral hemorrhagic septicemia virus (VHSV) infection.

    Science.gov (United States)

    Najib, Abdellaoui; Kim, Min Sun; Choi, Seung Hyuk; Kang, Yue Jai; Kim, Ki Hong

    2016-04-01

    To know the effect of viral hemorrhagic septicemia virus (VHSV) infection on the cellular microRNA expression profile in olive flounder (Paralichthys olivaceus), fish were infected with VHSV, and cellular microRNAs expression was analyzed at 0 (control), 6, 12, 24, 48 and 72 h post-infection (h.p.i.) by the high-throughput sequencing. A total of 372 mature miRNAs were identified, and, among them, 63 miRNAs were differentially expressed during VHSV infection. The differentially expressed microRNAs number was greatly increased from 24 h.p.i. compared to the number at 6 and 12 h.p.i., suggesting that the alteration of microRNAs expression by VHSV infection may be related to the progression of VHSV disease. The target prediction analysis, the GO enrichment analysis, and the KEGG pathway analysis of the predicted target genes showed that various biological pathways could be affected by VHSV infection through the down-regulation or up-regulation of host miRNAs. The present results provide a basic information on the microRNAs related to VHSV infection in olive flounder. Considering broad effects of microRNAs on various biological pathways, data in this study can be used to interpret the mechanism of VHSV pathogenesis, which, vice versa, can be used to develop control measures against VHSV. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Long-Term Trends in the Prevalence of Cancer and Other Major Diseases Among Flatfish in the Southeastern North Sea as Indicators of Changing Ecosystem Health

    NARCIS (Netherlands)

    Vethaak, A.D.; Jol, J.G.; Pieters, J.P.F.

    2009-01-01

    This paper analyses and discusses spatial and temporal patterns in the prevalence of major skin diseases (lymphocystis, epidermal hyperplasia/papilloma, ulcers), intestinal parasite Glugea sp., and liver cancer in dab (Limanda limanda) and flounder (Platichthys flesus) in the Dutch section of the

  1. Prevalence of viral haemorrhagic septicaemia virus in Danish marine fishes and its occurrence in new host species

    DEFF Research Database (Denmark)

    Skall, Helle Frank; Olesen, Niels Jørgen; Mellergaard, Stig

    2005-01-01

    to 1997. In total, 16 655 fish were examined virologically as 3569 samples. Forty fish species and 3 invertebrate species were represented. VHSV was isolated from 133 samples representing 8 species: herring Clupea harengus, sprat Sprattus sprattus, dab Limanda limanda, flounder Platichthys flesus, plaice...

  2. Detection of methanogenic archaea in seawater particles and the digestive tract of a marine fish species

    NARCIS (Netherlands)

    van der Maarel, MJEC; Sprenger, W; Haanstra, R; Forney, LJ

    1999-01-01

    A methanogen-specific nested PCR approach was used to detect methanogenic archaea in seawater particles of the North Sea and the feces and the digestive tract of flounder (Platichthys flesus), a fish found in the North Sea. A number of 16S rDNA sequences with 97.6-99.5% similarity to

  3. PCB contamination in fish community from the Gironde Estuary (France): blast from the past.

    Science.gov (United States)

    Bodin, N; Tapie, N; Le Ménach, K; Chassot, E; Elie, P; Rochard, E; Budzinski, H

    2014-03-01

    The contamination of the Gironde Estuary, southwest of France, by polychlorinated biphenyls (PCBs) was assessed using six fish of high ecological and economic importance as bioindicator species. The concentrations of 21 PCB congeners and total fat contents were determined in the muscle and liver of eels (Anguilla anguilla), seabass (Dicentrarchus labrax), flounders (Platichthys flesus), meagres (Argyrosomus regius), mullets (Liza ramada), and soles (Solea vulgaris). In addition, information regarding the trophic ecology of the studied fish was obtained through the analysis of carbon and nitrogen stable isotopes (i.e., δ(13)C and δ(15)N) in muscle. Results revealed high PCB concentrations in fish compared to monitored European estuaries. The muscle of eels was by far the most contaminated fish flesh (Σ7PCBs=1000±440 ng g(-1) on a dry weight basis), while the higher PCB concentrations in liver were measured in flounder (Σ7PCBs=2040±1160 ng g(-1) d.w.). A quantile regression approach allowed to investigate the fate of PCBs in the Gironde estuarine fish assemblage, and revealed a general process of trophic magnification. Finally, most of the analysed fish presented PCB concentrations in muscle meat above the current European maximum limits for sea products, while the derived "Toxic Equivalent Quantity" (TEQ) revealed human health concerns only for high-fat fish consumption. Copyright © 2014. Published by Elsevier Ltd.

  4. Mercury, Fatty Acids Content and Lipid Quality Indexes in Muscles of Freshwater and Marine Fish on the Polish Market. Risk Assessment of Fish Consumption

    Directory of Open Access Journals (Sweden)

    Joanna Łuczyńska

    2017-09-01

    Full Text Available Mercury content and fatty acids in muscles of Perca fluviatilis L. (European perch, Leuciscus idus L. (ide, Cyprinus carpio L. (European or common carp, Oncorhynchus mykiss Walb. (rainbow trout, Platichthys flesus L. (European flounder. and Clupea harengus L. (bream from the Polish market were investigated. The total mercury was processed with AAS. The fatty acids were analyzed by gas chromatography. The concentration of mercury in muscles varied from 0.006 to 0.138 mg/kg and decreased as follows: perch ≈ ide > flounder > herring ≈ bream ≈ rainbow trout > carp (p ≤ 0.05. There were only significant positive correlations between body weight and mercury content in muscle tissue of carp (r = 0.878, flounder (r = 0.925 and herring (r = 0.982 (p ≤ 0.05. The atherogenic index (AI, thrombogenicity index (TI and flesh-lipid quality index (FLQ were calculated as follows 0.33–0.70 (IA, 0.16–0.31 (IT and 13.01–33.22 (FLQ. Hypocholesterolemic (OFA and hypercholesterolemic fatty acids (DFA in muscles of fish ranged from 18.26 to 23.01 and from 73.91 to 78.46, respectively. In most cases, there were not significant correlations between size (body weight and total length and fatty acids in the muscles of the examined fish (p > 0.05. The Target Hazard Quotient (THQ values were below 1, which shows that there is no non-carcinogenic health risk to the consumer by consuming the examined fish.

  5. Poly(I:C induces antiviral immune responses in Japanese flounder (Paralichthys olivaceus that require TLR3 and MDA5 and is negatively regulated by Myd88.

    Directory of Open Access Journals (Sweden)

    Zhi-xia Zhou

    Full Text Available Polyinosinic:polycytidylic acid (poly(I:C is a ligand of toll-like receptor (TLR 3 that has been used as an immunostimulant in humans and mice against viral diseases based on its ability to enhance innate and adapt immunity. Antiviral effect of poly(I:C has also been observed in teleost, however, the underling mechanism is not clear. In this study, we investigated the potential and signaling mechanism of poly(I:C as an antiviral agent in a model of Japanese flounder (Paralichthys olivaceus infected with megalocytivirus. We found that poly(I:C exhibited strong antiviral activity and enhanced activation of head kidney macrophages and peripheral blood leukocytes. In vivo studies showed that (i TLR3 as well as MDA5 knockdown reduced poly(I:C-mediated immune response and antiviral activity to significant extents; (ii when Myd88 was overexpressed in flounder, poly(I:C-mediated antiviral activity was significantly decreased; (iii when Myd88 was inactivated, the antiviral effect of poly(I:C was significantly increased. Cellular study showed that (i the NF-κB activity induced by poly(I:C was upregulated in Myd88-overexpressing cells and unaffected in Myd88-inactivated cells; (ii Myd88 overexpression inhibited and upregulated the expression of poly(I:C-induced antiviral genes and inflammatory genes respectively; (iii Myd88 inactivation enhanced the expression of the antiviral genes induced by poly(I:C. Taken together, these results indicate that poly(I:C is an immunostimulant with antiviral potential, and that the immune response of poly(I:C requires TLR3 and MDA5 and is negatively regulated by Myd88 in a manner not involving NK-κB. These results provide insights to the working mechanism of poly(I:C, TLR3, and Myd88 in fish.

  6. Dietary Hizikia fusiformis glycoprotein-induced IGF-I and IGFBP-3 associated to somatic growth, polyunsaturated fatty acid metabolism, and immunity in juvenile olive flounder Paralichthys olivaceus.

    Science.gov (United States)

    Choi, Youn Hee; Kim, Kang-Woong; Han, Hyon-Sob; Nam, Taek Jeong; Lee, Bong-Joo

    2014-01-01

    This study was aimed to examine the effect of dietary glycoprotein extracted from the sea mustard Hizikia fusiformis (Phaeophyceae: Sargassaceae) as a dietary supplement on growth performance in association with somatotropin level, proximate compositions, and immunity in juvenile olive flounder Paralichthys olivaceus. Water-ethanol extracted glycoprotein from H. fusiformis was supplemented to three fishmeal-based diets at the concentration of 0, 5, and 10gkg(-1) diet (designated as H0, H5, and H10, respectively). After a 12week-long feeding trial, growth performance and biochemical responses were analyzed including proximate composition, and whole body amino acids and fatty acids. We also measured plasma insulin like growth factor (IGF), IGF-binding protein (IGFBP) and interleukin (IL). The fish fed H5 showed the greatest weight gain among the dietary treatments. In parallel with the growth, the fish fed the diets containing H. fusiformis glycoprotein showed an increased plasma IGF-I activity and increased expression of 43-kDa IGFBP-3 compared to that in the control, whereas an opposite trend was observed for 34-kDa IGFBP-1. Although no differences were found in the level of whole body linoleic acid (C18:2n-6) and linolenic acid (C18:3n-3) among treatments, increases in arachidonic acid (ARA, C20:4n-6), eicosapentaenoic acid (EPA, C20:5n-3) and docosahexaenoic acid (DHA, C22:6n-3) were observed in fish fed H5 compared to control. IL-2 and -6 levels increased significantly in fish fed H10 compared to those in the control indicating increased immunity. These results suggest that supplementation of H. fusiformis glycoprotein in fish diet may be beneficial for fish growth and immunity in juvenile olive flounder. © 2013.

  7. Inactivated vaccine against viral hemorrhagic septicemia (VHS) emulsified with squalene and aluminum hydroxide adjuvant provides long term protection in olive flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Vinay, Tharabenahalli-Nagaraju; Kim, Ye-Ji; Jung, Myung-Hwa; Kim, Wi-Sik; Kim, Do-Hyung; Jung, Sung-Ju

    2013-09-23

    Viral hemorrhagic septicemia (VHS) in olive flounder (Paralichthys olivaceus) remains an unsolved health problem in Korean aquaculture. Vaccination plays a significant role in modern aquaculture, and the duration of protection provided is of vital importance. Here, we have demonstrated the efficacy, duration of protection and safety of an inactivated vaccine emulsified with squalene (5%) and aluminum hydroxide (0.5%). The inactivated VHS vaccine provided a moderate protection of 37% and 47% relative percent survival (RPS) at 4 and 10 weeks post vaccination (wpv). Addition of squalene and aluminum hydroxide into inactivated VHS vaccine clearly enhanced the level of protection showing 58% and 83% RPS at 4 and 10 wpv, respectively, indicating the need for adjuvants to enhance the efficacy. The vaccinated fish showed significant protection at 3, 6, 12, 18, 24, and 40 wpv (except week 57) than non-vaccinated fish to an intraperitoneal challenge of 10(7.1)TCID₅₀/fish at 15 °C, with RPS of 60%, 64%, 71%, 55%, 52% and 50% (45% at 57 week), respectively, covering the duration of natural outbreak. Fish challenged at 18 wpv at 6 °C showed 56% RPS and protection at a low temperature. The antibody titer was high at 3 wpv with an OD of 1.08 ± 0.13, but decreased gradually and was undetectable by 24 wpv. The vaccine formulation was safe without injection site reactions, adhesions, or pigmentation observed at 6, 12, 18, or 24 wpv. Inflammatory reactions were observed in the spleen intestine at 6 and 12 wpv but were similar as control by 24 wpv. These results confirm that this vaccine is efficient and safe for olive flounder and could offer an appropriate strategy to prevent VHS without causing side effects. Copyright © 2013 Elsevier Ltd. All rights reserved.

  8. Anti-oxidative status and hepatic enzymes following acute administration of diethyl phthalate in olive flounder Paralichthys olivaceus, a marine culture fish.

    Science.gov (United States)

    Kang, Ju-Chan; Jee, Jung-Hoon; Koo, Ja-Geun; Keum, Yoo-Hwa; Jo, Soo-Gun; Park, Kwan Ha

    2010-09-01

    Although diethyl phthalate (DEP) is one of the most frequently used phthalates in solvents and fixatives for numerous industrial products, almost no research has been done on its biochemical toxicity in aquatic animals. Olive flounder (Paralichthys olivaceus), an important culture fish in far eastern Asian countries, were treated with intraperitoneal DEP at 0, 100, 300 or 900 mg/kg for three consecutive days and biochemical effects were assessed in the liver, kidney and serum 24 h after the final dosing. Measured parameters were mostly restricted to oxidative status and toxicity of the organs. In the hepatic tissue, there were significant increases in lipid peroxide (LPO) at 100mg/kg and above. Other hepatic parameters, which were examined, changed only after 900 mg/kg: reduced glutathione content (GSH), glutathione reductase activity (GR), glutathione peroxidase (GPx) activity increased; catalase (CAT) activity decreased. DEP also induced elevation in LPO levels at above 100 mg/kg in renal tissues; however, there was only a decrease in GR and glutathione S-transferase (GST) activities with DEP 900 mg/kg in contrast to the liver. Enzyme activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in hepatic tissues decreased in a dose-dependent manner in response to DEP at above 300 mg/kg. DEP at 300-900 mg/kg, although not uniform among parameters, caused increases in serum alkaline phosphatase (ALP), lactate dehydrogenase (LDH), AST, ALT activities and osmolality value, suggesting that DEP at these doses induced hepatic cell damage. The results indicate that 100-900 mg/kg DEP induced oxidative stress and the fish seemed to activate compensatory anti-oxidant systems to cope with the imposed substance on the liver. Such compensatory activation was not evident in the kidney. Overall, DEP was only weakly toxic to olive flounder in terms of oxidative and hepatic damage. Copyright (c) 2010 Elsevier Inc. All rights reserved.

  9. EUROPEAN AUSTERITY WITHOUT GROWTH? EUROPEAN GROWTH WITHOUT EUROPEAN DEMOCRACY?

    Directory of Open Access Journals (Sweden)

    Montani Guido

    2011-07-01

    Full Text Available The European project is facing a crisis. Citizens no longer understand what the EU is about. Young people and the new ruling class have forgotten the clear message of the European project launched just after the Second World War "No wars ever again among Europeans." The founding fathers of the European Union are mentioned in history textbooks, but today Europe is felt as an irritating bureaucracy. In Europe, peace and economic stability are considered as a natural state, a gift from above. Why keep a useless EU alive? The state of the European Union is swiftly degenerating. In almost all the member states, the anti-European forces are gaining ground. Populism is not a new ideology and is not necessarily European: let's recall Peronism. In today's Europe populism is the new manifestation of nationalism. In Italy the Lega Nord is in Berlusconi's eurosceptic government. In France, the National Front is endangering UMP's hegemony. In Belgium the rows between the Flemish and the Walloons threatens the state's unity. In the Netherlands, Hungary, the Czech Republic, Austria and Finland, populist forces are either in the government or strongly influencing the government. National-populism is different from the nationalism of the past. De Gaulle's nationalism was an ideology founded on the "grandeur" of France's history and on a certain idea of Europe, which was "l'Europe de patrie", a kind of European unity accepting French leadership in world politics. Today national-populism is a form of micro-nationalism: it opposes the European project but without having a serious alternative. This is why populism is dangerous. Its real goal is not only the breaking down of the European Union but also the disintegration of the old nation states into micro-ethnic states, as what happened in former Yugoslavia.

  10. Cryptobia neghmei sp. n. (Protozoa: Kinetoplastida in two species of flounder, Paralichthys spp. (Pisces: Paralichthydae off Chile Cryptobia neghmei sp. n (Protozoa: Kinetoplastida en dos especies de lenguados Paralichthys spp. (Pisces: Paralichthydae de la costa de Chile

    Directory of Open Access Journals (Sweden)

    RASUL A. KHAN

    2001-12-01

    Full Text Available Cryptobia neghmei sp.n. is described from the blood of two species of flounder, Paralichthys microps and P. adspersus, inhabiting the Chilean coast in the southern Pacific Ocean. Flagellates were elongate, slender, with two flagella and a conspicuous undulating membrane. It was distinguished from previously described species on the basis of its unusual shape and dimensions. All of 97 flounder were infected upon examination. Developmental stages of kinetoplastid protozoans, perhaps C. neghmei sp. n., were observed in some leeches Glyptonotobdella sp. that were found attached to flounder, which probably represent a mode for transmission among piscine hostsSe describe a Cryptobia neghmei sp.n., un protozoo sanguíneo de dos especies de lenguados, Paralichthys microps y P. adspersus, habitantes de la costa de Chile en el sur-este del océano Pacífico. Los protozoos flagelados son de forma elongada, delgados con dos flagelos y una membrana ondulante conspicua. Esta especie se distingue de aquellas descritas previamente en base a su forma y dimensiones inusuales. Los 97 lenguados revisados estaban infectados. Se observaron otros estados de desarrollo de protozoos kinetoplástidos en la sanguijuela Glyptonotobdella sp. que parasitaba a los lenguados y que probablemente sea el vector del protozoo

  11. Educating in European Identity?

    National Research Council Canada - National Science Library

    Enrique Banús

    2007-01-01

    In the last decades, the claim for a "European identity" has been manifested sometimes as a solution for the citizens' distance to the European project, sometimes also as a precondition for a further...

  12. Development of technologies to prevent fish diseases by vaccination. 1. Adaptation of an ELISA to detect serum antibody and the effect of the vaccination for Japanese flounder; Wakuchin ni yoru gyobyo yobo gijutsu no kaihatsu. 1. Hirame ni okeru erizaho no tekiyo to wakuchin toyo no yukosei

    Energy Technology Data Exchange (ETDEWEB)

    Furuta, T.; Honda, H.; Kikuchi, K.; Iwata, N.; Kiyono, M. [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1994-07-01

    An examination was carried out on the adaptation of an ELISA for flounder and on the preparation and effect of a protective vaccination against edwardsiellosis. The measurement of flounder antibody by ELISA was made possible by successively making a rabbit antibody and an enzyme tagged goat antibody react to the flounder antibody. By ELISA, the antibodies were able to be measured with a sensitivity 5 to 6 times higher compared with the conventional agglutination. The vaccination was prepared experimentally by producing edwardsiella tarda in aquaculture, adding formalin to inactivate it, and cleaning it with a physiological salt water. The vaccination thus prepared was administered to 4 groups of flounder consisting of those weighing 3, 17, 146 and 516g by means of injection or immersion. After that, the measurement was taken of antibody titers by ELISA, and a challenge test was performed by injecting edwardsiella tarda. A rise in antibody titers was observed in all sizes under the injection and in a part of the 516g group of flounders under the immersion. The effect of the administration was such that the increase in protective immunity was recognized under the injection but not under the immersion. 33 refs., 11 figs., 1 tab.

  13. INTERACTION BETWEEN DIETARY MINERAL AND PHYTASE ON BIOLOGICAL PERFORMANCES OF JAPANESE FLOUNDER, Paralichthys olivaceus. PART I. GROWTH, FEED INTAKE, AND WHOLE BODY MINERAL CONTENT

    Directory of Open Access Journals (Sweden)

    Asda Laining

    2013-06-01

    Full Text Available In order to determine the effect of dietary calcium (Ca, inorganic phosphorus (IP, and phytase (P supplementation in marine fish, a 2 x 2 x 2 factorial design was arranged to observe the interrelationship between dietary Ca and IP with the presence of dietary phytase. Two levels of dietary Ca at 0% and 0.2% combined with either 0% or 0.25% of dietary IP and either with 0 and 2,000 FTU phytase/kg diet, respectively to formulate eight experimental diets. SPI-based diet was used as basal diet and the sources of Ca, IP, and phytase were similar to those used in the previous experiment. Juvenile Japanese flounder with initial body weight around 0.6 g was fed the test diets. After 30 days of feeding trial, the results showed that both dietary IP and phytase, but not dietary Ca significantly enhanced the growth and feed intake. The highest growth was achieved in fish fed a diet containing the Ca, IP, and phytase supplement among groups. Fish fed diet without the three dietary supplements had the lowest SGR and did not significantly improve by supplementing dietary Ca. Feed intake (FI and was significantly influenced by dietary IP and phytase, not dietary Ca while feed conversion ratio (FCR was significantly affected by all dietary treatments. Interaction effect was detected between dietary Ca and IP, between dietary IP and P on FCR. Total phosphorus content in whole body was significantly increased by supplementing all dietary treatments which was highest in fish fed 0.25 IP/0.2 Ca/P. Significant interaction was observed between dietary IP and P on this parameter. Whole body Ca content was significantly improved by either dietary IP or Ca and not dietary P. As conclusion even without inorganic Ca supplement, dietary IP at level of 0.25% or 2,000 FTU phytase/kg diet could enhance growth and FI of fish as well as whole body phosphorus content of juvenile Japanese flounder when diet basal contained organic Ca around 1.2%.

  14. Europeanization and transnational states

    DEFF Research Database (Denmark)

    Jacobsson, Bengt; Lægreid, Per; Pedersen, Ove K.

    This work investigates what happens to an organized political unit when it becomes part of a larger entity and, in particular, how increased European integration and the tentative moves towards a transnational state will affect the European Union's nation state. Europeanization and the transforma......This work investigates what happens to an organized political unit when it becomes part of a larger entity and, in particular, how increased European integration and the tentative moves towards a transnational state will affect the European Union's nation state. Europeanization...... in central government agencies. It concludes that the consequences of Europeanization can be described as the growth of a transnational administration where identities as well as loyalties are created in processes that transcend the borders of states....

  15. Population Subdivision of Japanese Flounder Paralichthys olivaceus in the Pacific Coast of Tohoku Japan Detected by Means of Mitochondrial Phylogenetic Information

    Directory of Open Access Journals (Sweden)

    Yuya Shigenobu

    2013-01-01

    Full Text Available This study deals with mitochondrial phylogenetic information of Japanese flounder in the Pacific coast of Tohoku Japan to estimate the genetic population subdivision that was undetectable by conventional population statistics. We determined complete sequences of mitochondrial NADH dehydrogenase subunit-2 (ND2 and subunit-5 (ND5 genes for 151 individuals from northern (Aomori and Iwate prefectures, 40–41°N and southern (Miyagi and Fukushima prefectures, 37–38°N waters. Samples from both waters showed high genetic diversity, including 126 haplotypes. These haplotypes were located at mixed and nested positions on an inferred phylogenetic tree, and traditional F-statistics indicated no significant population divergence (φST = −0.00335, p > 0.05, corroborating our previous study. Three variable sites, however, showed significant base composition heterogeneity between samples from the northern and southern waters (Fisher’s exact-test, p < 0.01. Nucleotide substitutions at the three sites converged on an apical clade, which consisted of the five southern individuals, whereas its sister clade consisted only of the three northern individuals. This phylogenetic information corroborates previous ecological studies indicating the presence of separate stocks in the northern and southern waters.

  16. Reduction of nitrogen in the excretion on Japanese flounder using Ulva and Capitellid; Anaaosa to itogokai ni yoru hirame haisetsubutsuchu no chisso shori

    Energy Technology Data Exchange (ETDEWEB)

    Honda, H.; Kikuchi, K.; Sakaguchi, I. [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1997-03-01

    To develop the culture residue treatment technique using aquatic organisms, the ammonia and nitrate uptake rates of seaweed Ulva and the nitrogen reduction rate of polychaeta annelid Captella sp. with organic sediment predaceous ability were examined in the excretion of Japanese flounder. Nitrogen uptake rate of Ulva was affected by water temperature. It was highest at 20degC, followed at 15degC and 25degC in the order. It was not affected by light intensity between 1500 and 6000 lux. Ammonia and nitrate uptake rates by Ulva were estimated to be 28.2 and 14.6 {mu}g-N/g/h at 20degC under 3000 lux, respectively. Proportion of feces excreted from Capitellid to ingested sediments was 0.38. At 25degC, Capitellid population of one thousand individuals ingested-N at the rate of 24 mg-N/day, and excreted the feces-N of Capitellid at the rate of 7 mg-N/day. About 70% of nitrogen in the sediment was reduced through this process. 15 refs., 9 figs., 13 tabs.

  17. External Application of Fermented Olive Flounder (Paralichthys olivaceus) Oil Alleviates Inflammatory Responses in 2,4-Dinitrochlorobenzeneinduced Atopic Dermatitis Mouse Model.

    Science.gov (United States)

    Han, Sang-Chul; Kang, Gyeoung-Jin; Ko, Yeong-Jong; Kang, Hee-Kyoung; Moon, Sang-Wook; Ann, Yong-Seok; Yoo, Eun-Sook

    2012-09-01

    Allergic skin inflammation such as atopic dermatitis (AD) is characterized by edema and infiltration with various inflammatory cells such as mast cells, basophils, eosinophils and T cells. Thymic stromal lymphopoietin (TSLP) is produced mainly by epidermal keratinocytes, as well as dermal fibroblasts and mast cells in the skin lesions of AD. Omega-3 polyunsaturated fatty acids in fish oil can reduce inflammation in allergic patients. Fermentation has a tremendous capacity to transform chemical structures. The antiinflammatory effects of fish oil have been described in many diseases, but the beneficial effects by which fermented olive flounder oil (FOF) modulates the allergic response is poorly understood. In this study, we produced FOF and tested its ability to suppress the various allergic inflammatory responses. The ability of FOF to modulate the immune system was investigated using a mouse model of AD. The FOF-treated group showed significantly decreased immunoglobulin E (IgE) and histamine in serum. Also, the increased TSLP expression was significantly inhibited in the FOF group; the FOF-treated group was not appreciably different from the hydrocort cream treatment group. In addition, FOF treatment resulted in a smaller spleen size with reduced the thickness and length compared to the induction group. Splenocytes from mice treated with FOF produced significantly less IFN-γ, IL-4, T-box transcription factor (T-bet) and GATA binding protein 3 (GATA3) expression compared with the induction group. These results suggest that FOF may be effective in treating the allergic symptoms of AD. 5.

  18. Anti-Microbial, Anti-Biofilm Activities and Cell Selectivity of the NRC-16 Peptide Derived from Witch Flounder, Glyptocephalus cynoglossus

    Directory of Open Access Journals (Sweden)

    Chang Ho Seo

    2013-05-01

    Full Text Available Previous studies had identified novel antimicrobial peptides derived from witch flounder. In this work, we extended the search for the activity of peptide that showed antibacterial activity on clinically isolated bacterial cells and bacterial biofilm. Pseudomonas aeruginosa was obtained from otitis media and cholelithiasis patients, while Staphylococcus aureus was isolated from otitis media patients. We found that synthetic peptide NRC-16 displays antimicrobial activity and is not sensitive to salt during its bactericidal activity. Interestingly, this peptide also led to significant inhibition of biofilm formation at a concentration of 4–16 μM. NRC-16 peptide is able to block biofilm formation at concentrations just above its minimum inhibitory concentration while conventional antibiotics did not inhibit the biofilm formation except ciprofloxacin and piperacillin. It did not cause significant lysis of human RBC, and is not cytotoxic to HaCaT cells and RAW264.7 cells, thereby indicating its selective antimicrobial activity. In addition, the peptide’s binding and permeation activities were assessed by tryptophan fluorescence, calcein leakage and circular dichroism using model mammalian membranes composed of phosphatidylcholine (PC, PC/cholesterol (CH and PC/sphingomyelin (SM. These experiments confirmed that NRC-16 does not interact with any of the liposomes but the control peptide melittin did. Taken together, we found that NRC-16 has potent antimicrobial and antibiofilm activities with less cytotoxicity, and thus can be considered for treatment of microbial infection in the future.

  19. Latitudinal and stock-specific variation in size- and age-at-maturity of female winter flounder, Pseudopleuronectes americanus, as determined with gonad histology

    Science.gov (United States)

    McBride, Richard S.; Wuenschel, Mark J.; Nitschke, Paul; Thornton, Grace; King, Jeremy R.

    2013-01-01

    Female winter flounder were examined using gonad histology to determine the adequacy of routine macroscopic maturity classification methods and to determine the spatial variation in size and age of maturity in U.S. waters. Sampling occurred in spring and autumn, which was adequate to collect immature, mature, spawning-active, and non-active females. Females were collected in coastal waters from Delaware Bay, USA, to the Scotian Shelf, Canada, including in Long Island Sound and on Georges Bank, which covered all U.S. stock areas. Mature fish spawned in spring, when gonads comprised up to 30% of the total body weight. Direct comparisons of maturity assignment by macroscopic versus microscopic methods demonstrated that both schemes are compatible, but the more cost-effective macroscopic method had trouble distinguishing larger immature from smaller resting females. Spatial comparisons, using gonad histology only, supported the existence of three stocks in U.S. waters, but also revealed significant variation in age at maturity within the two coastal stocks. Age-at-maturity was more variable than size-at-maturity, which is consistent with known stock-specific patterns of growth rates and a postulated life history tradeoff to delay maturity until a size threshold is reached. The within-stock variation in median age at maturity, about one year for coastal stocks, recommends further investigation of using static, stock-specific maturity ogives to calculate reference points for management.

  20. THE EUROPEAN BUSINESS CYCLE

    Directory of Open Access Journals (Sweden)

    Ignat Ion

    2009-05-01

    Full Text Available The construction of European Monetary Union has raised several questions about the existence of a common business cycle, a European one. The lack of cyclical synchronization would complicate the monetary and fiscal policies in the Union, being a negative

  1. European Home Energy

    DEFF Research Database (Denmark)

    Tommerup, Henrik M.

    2009-01-01

    An important aim of the european energy performance of buildings directive is to improve the overall energy efficiency of new homes......An important aim of the european energy performance of buildings directive is to improve the overall energy efficiency of new homes...

  2. European Industry, 1700 - 1870

    NARCIS (Netherlands)

    Broadberry, Stephen; Fremdling, Rainer; Solar, Peter M.

    2008-01-01

    This paper offers an overview of the development of European industry between 1700 and 1870, drawing in particular on the recent literature that has emerged following the formation of the European Historical Economics Society in 1991. The approach thus makes use of economic analysis and quantitative

  3. The European Programme Manager

    DEFF Research Database (Denmark)

    Larson, Anne; Bergman, E.; Ehlers, S.

    The publication is a result of a cooperation between organisations in six European countries with the aim to develop a common European education for programme managers. It contains of a description of the different elements of the education together with a number of case-studies from the counties...

  4. European works councils

    DEFF Research Database (Denmark)

    Knudsen, Herman Lyhne

    2003-01-01

    The theme adressed by this paper is the opportunities for European Works Councils (EWCs) of gaining influence on corporate decisions in multinational companies.......The theme adressed by this paper is the opportunities for European Works Councils (EWCs) of gaining influence on corporate decisions in multinational companies....

  5. European works councils

    DEFF Research Database (Denmark)

    Knudsen, Herman Lyhne

    2004-01-01

    The theme addressed by this artcle is the opportunities for European Works Councils of gaining influence on corporate decisions in multinational companies.......The theme addressed by this artcle is the opportunities for European Works Councils of gaining influence on corporate decisions in multinational companies....

  6. Introduction: European climate leadership

    NARCIS (Netherlands)

    Wurzel, R.K.W.; Liefferink, J.D.; Connelly, J.

    2017-01-01

    There is no shortage of would-be leaders in EU climate change politics. The EU institutions (e.g. European Council, Council of the EU, Commission and the European Parliament (EP)), member states and societal actors have all, though to varying degrees and at different time periods, tried to offer

  7. Europeanization : A Poststructuralist Approach

    NARCIS (Netherlands)

    Neuman-Stanivukovic, Senka

    2014-01-01

    This dissertation begins from the hypothesis that the direction and extent of Europeanization depends on domestic perception of the European Union and its norms. Accordingly, it was my empirical ambition to study how competing articulations of the EU and its norms constructed debates in EU member

  8. European media law

    NARCIS (Netherlands)

    Castendyk, O.; Dommering, E.; Scheuer, A.

    2008-01-01

    European Union legislation concerning electronic communications media is firmly established as an essential part of the law in the field in Europe. From relevant provisions of the European Convention of Human Rights and the EC Treaty to numerous directives, the most recent being the Audiovisual

  9. Co-occurrence of musk fragrances and UV-filters in seafood and macroalgae collected in European hotspots.

    Science.gov (United States)

    Cunha, S C; Fernandes, J O; Vallecillos, L; Cano-Sancho, G; Domingo, J L; Pocurull, E; Borrull, F; Maulvault, A L; Ferrari, F; Fernandez-Tejedor, M; Van den Heuvel, F; Kotterman, M

    2015-11-01

    In the last decades, awareness regarding personal care products (PCP), i.e. synthetic organic chemicals frequently used in cosmetic and hygienic products, has become a forward-looking issue, due to their persistency in the environment and their potential multi-organ toxicity in both human and wildlife. Seafood is one of the most significant food commodities in the world and, certainly, one of the most prone to bioaccumulation of PCP, what can consequently lead to human exposure, especially for coastal population, where its consumption is more marked. The aim of this work was to evaluate the co-occurrence of musk fragrances and UV-filters in both seafood and macroalgae collected in different European hotspots (areas with high levels of pollution, highly populated and near wastewater treatment plants). Despite the fact that UV-filters were detected in three different kind of samples (mussel, mullet, and clam), in all cases they were below the limit of quantification. Galaxolide (HHCB) and tonalide (AHTN) were the musk fragrances most frequently detected and quantified in samples from the European hotspots. Cashmeran (DPMI) was also detected in most samples but only quantified in two of them (flounder/herring and mullet). The highest levels of HHCB and AHTN were found in mussels from Po estuary. Copyright © 2015 Elsevier Inc. All rights reserved.

  10. Molecular identification and transmission studies of X-cell parasites from Atlantic cod Gadus morhua (Gadiformes: Gadidae and the northern black flounder Pseudopleuronectes obscurus (Pleuronectiformes: Pleuronectidae

    Directory of Open Access Journals (Sweden)

    Watanabe K

    2011-02-01

    Full Text Available Abstract Background Epidermal pseudotumours from Hippoglossoides dubius and Acanthogobius flavimanus in Japan and gill lesions in Limanda limanda from the UK have been shown to be caused by phylogenetically related protozoan parasites, known collectively as X-cells. However, the phylogenetic position of the X-cell group is not well supported within any of the existing protozoan phyla and they are currently thought to be members of the Alveolata. Ultrastructural features of X-cells in fish pseudotumours are somewhat limited and no typical environmental stages, such as spores or flagellated cells, have been observed. The life cycles for these parasites have not been demonstrated and it remains unknown how transmission to a new host occurs. In the present study, pseudobranchial pseudotumours from Atlantic cod, Gadus morhua, in Iceland and epidermal pseudotumours from the northern black flounder, Pseudopleuronectes obscurus, in Japan were used in experimental transmission studies to establish whether direct transmission of the parasite is achievable. In addition, X-cells from Atlantic cod were sequenced to confirm whether they are phylogenetically related to other X-cells and epidermal pseudotumours from the northern black flounder were analysed to establish whether the same parasite is responsible for infecting different flatfish species in Japan. Results Phylogenetic analyses of small subunit ribosomal DNA (SSU rDNA sequence data from Atlantic cod X-cells show that they are a related parasite that occupies a basal position to the clade containing other X-cell parasites. The X-cell parasite causing epidermal pseudotumours in P. obscurus is the same parasite that causes pseudotumours in H. dubius. Direct, fish to fish, transmission of the X-cell parasites used in this study, via oral feeding or injection, was not achieved. Non-amoeboid X-cells are contained within discrete sac-like structures that are loosely attached to epidermal pseudotumours in

  11. Effects of Dietary Lipid Source and Level on Growth Performance, Blood Parameters and Flesh Quality of Sub-adult Olive Flounder (

    Directory of Open Access Journals (Sweden)

    Dong-Kyu Kim

    2012-06-01

    Full Text Available This study was conducted to investigate the effects of dietary lipid source and level on growth performance, blood parameters, fatty acid composition and flesh quality of sub-adult olive flounder Paralichthys olivaceus. Eight experimental diets were formulated to contain 5% squid liver oil (SLO, 5% linseed oil (LO, 5% soybean oil (SO, a mixture of 1% squid liver oil, 2% linseed oil and 2% soybean oil (MIX, no lipid supplementation with high protein level (LL-HP, 10% squid liver oil (HL-SLO, a mixture of 1% squid liver oil, 4.5% linseed oil and 4.5% soybean oil (HL-VO, and 1% squid liver oil with high starch level (LL-HC, respectively. Two replicate groups of fish (average initial weight of 296 g were fed the diets for 17 wks. After 5 wks, 11 wks and the end of the feeding trial, five fish from each tank were randomly sampled for analysis of body composition. At the end of the feeding trial, final mean weight of fish fed the LL-HP diet was significantly (p<0.05 higher than that of fish fed the HL-VO diet, but did not differ significantly from those of fish fed the SLO, LO, SO, MIX, HL-SLO and LL-HC diets. Fish fed the LL-HP diet showed significantly higher feed efficiency than fish fed the LO, HL-SLO and HL-VO diets. Feed efficiency of fish fed the LO, SO and MIX diets were similar to those of fish fed the SLO and HL-SLO diets. Fish fed the HL-SLO diet showed significantly higher total cholesterol content in plasma compared with other diets. Fatty acid composition of tissues was reflected by dietary fatty acid composition. The highest linoleic (LA and linolenic acid (LNA contents in the dorsal muscle were observed in fish fed the SO and LO diets, respectively, regardless of feeding period. The highest eicosapentaenoic acid (EPA content in the dorsal muscle was observed in fish fed the LL-HP and LL-HC diets after 11 and 17 weeks of feeding, respectively. Fish fed the SLO and HL-SLO diets showed higher docosahexaenoic acid (DHA content than that of

  12. Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature

    Science.gov (United States)

    Liu, Yifan; Ma, Daoyuan; Xiao, Zhizhong; Xu, Shihong; Wang, Yanfeng; Wang, Yufu; Xiao, Yongshuang; Song, Zongcheng; Teng, Zhaojun; Liu, Qinghua; Li, Jun

    2015-01-01

    High temperature influences the homeostasis of fish. We investigated the effects of elevated temperature on tissues of Japanese flounder ( Paralichthys olivaceus) by analyzing the histology and heat shock protein 70 ( hsp70) expression of fish reared in warm conditions. In this study, temperature was increased at 1±0.5°C/day starting at 24±0.5°C, and was kept at that temperature for 5 days before the next rise. After raising temperature at the rate up to 32±0.5°C, tissue samples from midgut, spleen, stomach, liver, muscle, gill, heart, trunk kidney and brain were collected for histological analysis and mRNA assay. Almost all the tissues showed changes in morphological structure and hsp70 level at 32±0.5°C. Histological assessment of the tissues indicated that the gill had the most serious damage, including highly severe epithelial lifting and edema, curved tips and hyperemia at the ending of the lamellars, desquamation and necrosis. The next most severe damage was found in liver and kidney. The hsp70 levels in all the tissues first increased and then decreased. The gut, stomach, muscle, heart, and brain had the highest expressions in 6 h, whereas the spleen, liver, gill and kidney had the highest expressions in 2 h. Therefore, tissues with the most significant lesions (especially gill and liver) responded much earlier (2 h) in hsp70 expression than other tissues, and these tissues demonstrated the most marked histological disruption and elevated mRNA levels, making them ideal candidates for further studies on the thermal physiology of this species.

  13. Development of a stringent ELISA protocol to evaluate anti-viral hemorrhagic septicemia virus-specific antibodies in olive flounder Paralichthys olivaceus with improved specificity.

    Science.gov (United States)

    Kim, Hyoung Jun; Park, Jeong Su; Kwon, Se Ryun

    2015-07-01

    Olive flounder were vaccinated with polyinosinic:polycytidylic acid [Poly (I:C)] to prevent viral hemorrhagic septicemia (VHS). Vaccine efficacy was verified by detection of anti- VHS virus (VHSV) antibodies using enzyme-linked immunosorbent assay (ELISA). In the study, ELISA absorbance values of the negative control group [Poly (I:C)-MEM10] were saturated when an ELISA protocol, that includes pretreatment of the fish sera with 5% skim milk, was used. However, the saturated OD values in the negative control did not correlate with a specific immune response against VHSV, because the group showed low survival rate (only 10%) following the VHSV challenge. Also, OD values of Poly (I:C)- VHSV group were high, and the group showed high survival rate (97.5%) against VHSV challenge test. It was suggested that the high OD values were possibly due to the presence of anti-fetal bovine serum (FBS) cross-reactivity. To compensate this, we subtracted the absorbance of infectious hematopoietic necrosis (IHNV)-Ag plates from those of the VHSV-Ag plates. However, the average value for the Poly (I:C)-VHSV group (0.167) was lower than expected even though high survival rate. We used an advanced ELISA system to pre-treat fish sera with 5% skim milk and two novirhabdoviruses as capture antigens as well as 50% FBS. The corrected absorbance values for pre-treated fish sera from the negative control Poly (I:C)-MEM10 and experimental Poly (I:C)-VHSV groups averaged 0.033 and 0.579, respectively. The specific VHSV antibody response of the vaccinated group was assessed using fish sera pretreated with skim milk and FBS and by calculating the corrected absorbance values from ELISA with two novirhabdovirus capture antigens.

  14. Update of European bioethics

    DEFF Research Database (Denmark)

    Rendtorff, Jacob Dahl

    2015-01-01

    , the principles of autonomy, dignity, integrity and vulnerability are proposed as the most important ethical principles for respect for the human person in biomedical and biotechnological development. This approach to bioethics and biolaw is presented here in a short updated version that integrates the earlier......This paper presents an update of the research on European bioethics undertaken by the author together with Professor Peter Kemp since the 1990s, on Basic ethical principles in European bioethics and biolaw. In this European approach to basic ethical principles in bioethics and biolaw...... research in a presentation of the present understanding of the basic ethical principles in bioethics and biolaw....

  15. Democratic Citizenship: European referents

    Directory of Open Access Journals (Sweden)

    María PUIG GUTIÉRREZ

    2011-09-01

    Full Text Available Let’s sense beforehand in this article a tour concerning the educational European policies that favors the development of a democratic citizenship. The aim that we chase is to understand the way in which nowadays it is being interpreted and stimulated the Citizenship education from European Union. for it we offer a conceptual delimiting of «Citizenship education» and later, we show an analysis of the principal documents and materials elaborated principally by the Council of Europe that mark the way followed by European Union as for education for Democratic Citizenship (EDC.

  16. CERN welcomes European science

    CERN Multimedia

    2002-01-01

    On 3 and 4 October CERN will host a special workshop for Marie Curie fellows. This programme is a key plank in the EU's strategy for creating a European research area.     With thousands of scientists from all over the continent working together, CERN is already an exemplary European science showcase. On 3 and 4 October, the Laboratory will contribute further to unifying all European science by hosting a special workshop for EU-funded Marie Curie fellows. This scheme gives young researchers from around the continent the mobility to go to wherever Europe's best facilities in their chosen field happen to be. The event that will take place at CERN, entitled 'Special workshop of Marie Curie Fellows on research and training in physics and technology', organised together with the European Commission, is a continuation of a series of workshops with the aim, among others, of promoting young researchers, supporting their training and mobility, and facilitating the interdisciplinary dissemination of knowledge. Dur...

  17. European Economic Integration

    Science.gov (United States)

    Huston, James A.

    1971-01-01

    Recounts the history and problems of European Economic Integration from the first post World War II organization, the OEEC, to the EEC (Common Market) and the EFTA. Suggestions for further reading are included. (JB)

  18. European Molecular Biology Laboratory

    CERN Multimedia

    1973-01-01

    On 10 May an Agreement was signed at CERN setting up a new European Laboratory. It will be concerned with research in molecularbiology and will be located at Heidelberg in the Federal Republic of Germany.

  19. The European University Landscape

    DEFF Research Database (Denmark)

    Daraio, Cinzia; Bonaccorsi, Andrea; Geuna, Aldo

    2011-01-01

    This paper provides a new and systematic characterization of 488 universities, from 11 European countries: Finland, France, Germany, Hungary, Italy, Netherlands, Norway, Portugal, Spain, Switzerland and UK. Using micro indicators built on the integrated Aquameth database, we characterize the Euro...

  20. European mobility cultures

    DEFF Research Database (Denmark)

    Haustein, Sonja; Nielsen, Thomas A. Sick

    2016-01-01

    More targeted European policies promoting green travel patterns require better knowledge on differing mobility cultures across European regions. As a basis for this, we clustered the EU population into eight mobility styles based on Eurobarometer data. The mobility styles - including, for example......-economic resources. In a second step, the 28 EU member countries were clustered into six country clusters based on their representation of mobility styles. The country clusters indicate the existence of considerably different mobility cultures across the EU. Sub-regions can be identified that have highly different...... positions on the path towards sustainable mobility and therefore different requirements towards European platforms and support measures, e.g. for 'Sustainable Urban Mobility Plans'. The country clusters can provide a starting point for future communication and targeting of European efforts in sustainable...

  1. European Cyber Security Policy

    OpenAIRE

    Bendiek, Annegret

    2012-01-01

    The gradually developing European cyber security policy tries to establish minimum standards in all EU member states with regard to prevention, resilience and international cooperation. It aims to foster national security without compromising democratic principles or unduly limiting individual liberties. However, it is hard to find a balance between these goals, and the EU’s measures thus inevitably raise questions about the democratic implications of European cyber security policy. Are the i...

  2. Timetabling on European Corridors

    OpenAIRE

    Lischke, Andreas; Führer, B. (Bernhard); Garavagno, G.

    2005-01-01

    The Path Allocation Re-engineering of Timetable Networks for European Railways (PARTNER) project aims to demonstrate a new way of train path allocation and assembly along international corridors towards a faster and more coordinated railway infrastructure capacity management. PARTNER will assist two neighbour infrastructure managers to develop a common understanding of the effects of international train paths. The results of a survey of European infrastructure managers and railway underta...

  3. European Physical Society awards

    CERN Multimedia

    2004-01-01

    The winners of the 2004 Accelerator Prizes, awarded by the European Physical Society's Interdivisional Group on Accelerators (EPS-IGA), have been announced. Vladmir Shiltsev (Fermilab) and Igor Meshkov (JINR, Joint Institute of Nuclear Research, Dubna) will be presented with their awards during the 9th European Particle Accelerator Conference, EPAC'04, on 8 July 2004 in Lucerne. Both physicists will also give a talk about their work. More details on: http://epac.web.cern.ch/

  4. European Banking Union

    OpenAIRE

    Breuss, Fritz

    2013-01-01

    The ongoing Euro crisis and the worse economic development in Europe than in the USA are grounded, not the least in the delayed implementation of reforms of the banking sector. Whereas the leaks in economic governance of EMU have been fixed the banking sector is still not stabilised, even five years after Lehman Brothers. From the grand solution of a "European Banking Union" (EBU) only the first pillar, the European Bank Supervision with the single supervisory mechanism (SSM) will come into e...

  5. European DHC Research Issues

    Energy Technology Data Exchange (ETDEWEB)

    Wiltshire, Robin; Williams, Jonathan (Building Research Establishment, BRE, Bucknalls Lane, Watford (United Kingdom)); Werner, Sven (Halmstad University, School of Business and Engineering, Halmstad (Sweden))

    2008-09-15

    Euroheat and Power is now working towards a European Technology Platform for District Heating and Cooling. In response to this important European DHC research initiative, a preliminary detailed list of research issues within 18 dimensions was elaborated and communicated to more than 100 people in February 2008. After additions and comments received, an updated list of research issues was again distributed in July 2008. This paper contains the current list of suggested research issues

  6. An integrated approach to study the biomarker responses in marine gastropod Nerita chamaeleon environmentally exposed to polycyclic aromatic hydrocarbons

    Digital Repository Service at National Institute of Oceanography (India)

    Bhagat, J.; Sarkar, A.; Deepti, V.; Singh, V.; Raiker, L.; Ingole, B.S.

    are well documented in marine organisms (Niyogi et al., 2001a, b; Pan et al., 2006). Among the oxidative stress biomarkers superoxide dismutase (SOD), catalase (CAT), Glutathione S- transferase (GST), and lipid peroxidation (LPO) have been widely used... information regarding mechanisms of biological effects of contaminants (Marigómez et al., 2013). Beliaeff and Burgeot (2002) were the first to construct star plot using IBR values in flounder Platichthys flesus using EROD, GST, CAT, AChE enzymatic...

  7. Habermas on European Constitution and European Identity

    Directory of Open Access Journals (Sweden)

    Éva Biró-Kaszás

    2010-12-01

    Full Text Available For the last two decades or so philosophers have been reflecting on a set of practical and political concerns in connection with the new political structural arrangements beyond the nation-state. In this article two essays by Jürgen Habermas shall be examined. An attempt shall be made to tackle Habermas’ philosophical concepts of personal and collective identity as well as the role that a constitution may play in building the post-national constellation. It has been shown that Habermas has normative answers. Firstly, according to him, the fragile balance between the legal order and the particular cultures and traditions of a community has to be protected by the constitutional state. For that reason the political culture has to be “decoupled” from the majority culture. Secondly, the democratically structured attempt to achieve shared meaning has to find the delicate balance between the context-transcending universal normative claims and the claims of particular individual and collective life. Thirdly, it is possible to expand legally mediated civil solidarity trans-nationally, across Europe – we may recognize this development as the emergence of European identity –, since the process of democratic will-formation of citizens may get loose from the structures provided by the state if both shared democratic political cultures as well as a European-wide public sphere exist. The European Constitution may have a catalytic function in materialization of these conditions. It has been shown that in his deliberations Habermas tried to find a reflective equilibrium between the normative and the empirical.

  8. Corrigendum to “Persistent organic pollutants in the Scheldt estuary: Environmental distribution and bioaccumulation[Environ. Int. 48C (2012) 17–27].

    Science.gov (United States)

    Van Ael, Evy; Covaci, Adrian; Blust, Ronny; Bervoets, Lieven

    2014-02-01

    Levels of polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and organochlorine pesticides (OCPs) were determined in the sediment and in several species (European flounder,Platichthys flesus; common sole, Solea solea; Chinese mitten crab,Eriocheir sinensis; shore crab, Carcinus maenas; brown shrimp,Crangon crangon; blue mussel, Mytilus edulis and bristle worms,Polychaeta) from 7 locations in the Scheldt estuary (SE, the Netherlands–Belgium). Overall POP levels in the sediment were low. The average PCB and PBDE concentrations were respectively 31.5 and 115 ng/g dry weight (dw). The highest sediment loads were measured in the vicinity of Antwerp (368 ng PCBs/g dw),a location with intense harbor and industrial activities. Pollution concentrations in the tissues of biota were species-specific. European flounder reached the highest contamination levels (from 12.9 to285 ng PCBs/g ww, from 0.25 to 3.97 ng PBDEs/g ww). The lowest tissue loads were measured in brown shrimp (from 3.27 to 39.9 ngPCBs/g ww, from 0.05 to 0.47 ng PBDEs/g ww). The PCB congener profile in most of the species was similar to the pattern found in the sediment. PCB 153 was the most abundant congener (16.5–25.7% in biota, 10.4% in sediment). In the sediment, the total amountof PBDEs consisted of more than 99% of BDE 209. Congener BDE 47had the highest concentrations in all sampled species (38.5–70.1%).Sediment POP loadings and tissue concentrations were poorly correlated,indicating that a simple linear or non-linear relationship is insufficient to describe this relationship, possible caused by the complexity of the bioaccumulation processes and the variability in exposure.Because of the high PCB levels, regular consumption of fish and seafood, especially mussels, from the Scheldt estuary should be avoided.

  9. The integrated analysis of RNA-seq and microRNA-seq depicts miRNA-mRNA networks involved in Japanese flounder (Paralichthys olivaceus) albinism.

    Science.gov (United States)

    Wang, Na; Wang, Ruoqing; Wang, Renkai; Tian, Yongsheng; Shao, Changwei; Jia, Xiaodong; Chen, Songlin

    2017-01-01

    Albinism, a phenomenon characterized by pigmentation deficiency on the ocular side of Japanese flounder (Paralichthys olivaceus), has caused significant damage. Limited mRNA and microRNA (miRNA) information is available on fish pigmentation deficiency. In this study, a high-throughput sequencing strategy was employed to identify the mRNA and miRNAs involved in P. olivaceus albinism. Based on P. olivaceus genome, RNA-seq identified 21,787 know genes and 711 new genes by transcripts assembly. Of those, 235 genes exhibited significantly different expression pattern (fold change ≥2 or ≤0.5 and q-value≤0.05), including 194 down-regulated genes and 41 up-regulated genes in albino versus normally pigmented individuals. These genes were enriched to 81 GO terms and 9 KEGG pathways (p≤0.05). Among those, the pigmentation related pathways-Melanogenesis and tyrosine metabolism were contained. High-throughput miRNA sequencing identified a total of 475 miRNAs, including 64 novel miRNAs. Furthermore, 33 differentially expressed miRNAs containing 13 up-regulated and 20 down-regulated miRNAs were identified in albino versus normally pigmented individuals (fold change ≥1.5 or ≤0.67 and p≤0.05). The next target prediction discovered a variety of putative target genes, of which, 134 genes including Tyrosinase (TYR), Tyrosinase-related protein 1 (TYRP1), Microphthalmia-associated transcription factor (MITF) were overlapped with differentially expressed genes derived from RNA-seq. These target genes were significantly enriched to 254 GO terms and 103 KEGG pathways (p<0.001). Of those, tyrosine metabolism, lysosomes, phototransduction pathways, etc., attracted considerable attention due to their involvement in regulating skin pigmentation. Expression patterns of differentially expressed mRNA and miRNAs were validated in 10 mRNA and 10 miRNAs by qRT-PCR. With high-throughput mRNA and miRNA sequencing and analysis, a series of interested mRNA and miRNAs involved in fish

  10. Transnational European Television Drama

    DEFF Research Database (Denmark)

    Bondebjerg, Ib; Redvall, Eva Novrup; Helles, Rasmus

    This book deals with the role of television drama in Europe as enabler of transnational, cultural encounters for audiences and the creative community. It demonstrates that the diversity of national cultures is a challenge for European TV drama but also a potential richness and source of creative...... variation. Based on data on the production, distribution and reception of recent TV drama from several European countries, the book presents a new picture of the transnational European television culture. The authors analyse main tendencies in television policy and challenges for national broadcasters...... coming from new global streaming services. Comparing cases of historical, contemporary and crime drama from several countries, this study shows the importance of creative co-production and transnational mediated cultural encounters between national cultures of Europe....

  11. The Europeanization of Eurosceptics?

    DEFF Research Database (Denmark)

    Dutceac Segesten, Anamaria; Bossetta Jr, Michael Joseph

    with the case of Sweden. Sweden is a good example because it has a high density of internet use, a politically active population as reflected in voter turnout and many politicians and political parties with a web presence. The main research questions of the paper are: Which actors are involved in the Swedish...... of a Europeanized network of anti-European voices for European integration and democracy. We plan to operationalize our research question through the use of webcrawling (i.e. Issue Crawler) and social media analytic softwares (Twitonomy and Bluenod). Using these softwares we will be able to discern whether...... or not non-Swedish Twitter users are participating in and/or affecting the Swedish Eurosceptic dialogue on social media....

  12. European Analytical Column

    DEFF Research Database (Denmark)

    Karlberg, B.; Grasserbauer, M.; Andersen, Jens Enevold Thaulov

    2009-01-01

    The European Analytical Column has once more invited a guest columnist to give his views on various matters related to analytical chemistry in Europe. This year, we have invited Professor Manfred Grasserbauer of the Vienna University of Technology to present some of the current challenges...... for European analytical chemistry. During the period 2002–07, Professor Grasserbauer was Director of the Institute for Environment and Sustainability, Joint Research Centre of the European Commission (EC), Ispra, Italy. There is no doubt that many challenges exist at the present time for all of us representing...... a major branch of chemistry, namely analytical chemistry. The global financial crisis is affecting all branches of chemistry, but analytical chemistry, in particular, since our discipline by tradition has many close links to industry. We have already noticed decreased industrial commitment with respect...

  13. European Universe Awareness

    Science.gov (United States)

    Russo, P.; Miley, G.; Westra van Holthe, F.; Schrier, W.; Reed, S.

    2011-10-01

    The European Universe Awareness (EU-UNAWE) programme uses the beauty and grandeur of the cosmos to encourage young children, particularly those from underprivileged backgrounds, to develop an interest in science and technology and to foster a sense of global citizenship. EU-UNAWE is already active in 40 countries and comprises a global network of almost 500 astronomers, teachers and other educators. The programme was recently awarded a grant of 1.9 million euros by the European Union so that it can be further developed in five European countries and South Africa. The grant will be used to organise teacher training workshops and to develop educational materials, such as an astronomy news service for children and games. During this presentation we will outline some of the biggest achievements of EU-UNAWE to date and discuss future plans for the programme.

  14. The European Identity

    Directory of Open Access Journals (Sweden)

    Alberto Martinelli

    2017-09-01

    Full Text Available European identity is not only a scientifically interesting question, but also a politically important issue: in fact, sixty years after the signing of the Treaty of Rome, the European Union finds itself for the first time facing risks that threaten its own existence. The European Union is a limited and incomplete project because Europe’s economic integration has not been accompanied by a genuine supranational political union and greater cultural integration. The deficit of democratic representation and cultural integration is due to the fact that the community process is based only on economic rationality and not on a feeling of common belonging. In the current situation in which the Union faces difficult challenges which threaten to undermine the future, it necessary to affirm the policy of interests with a policy of identity. In this essay, we will first concentrate on the concept of identity – that is on the nucleus of values and common institutions –; then we will discuss how the European identity has changed over time (also in relation to national identities and what are the mechanisms that may favour its taking root in the current situation. The European project of political unification needs to be re-emphasized, finding the way to a European collective identity, not contrasted with but alongside the different national identities, referring to loyalty and shared commitment to a whole collection of cultural values: fundamental human rights, civil liberties, democratic political institutions, rule of law, freedom of movement of people, goods and capital, social justice and non-violent resolution of conflicts.

  15. European Gas Dependency

    OpenAIRE

    Antonsen, Iben; Bieber, Martin; Gandrup, Tobias; Lehmann, Tina; Weinberger, Ashley

    2008-01-01

    The focal point of the project is, as it is implied in the title, European gas dependency, to be more specific; the dependency of Russian gas. We chose Russia, because the EU's import of gas is mainly supplied by Russia. We present background information and knowledge to describe why the case is of relevance and that a situation of dependency exists. We solely look at the dependency from the European Union's point of view, taking point of departure in the Green Paper. The Green Paper is a pap...

  16. European Values and Globalization

    Directory of Open Access Journals (Sweden)

    Heinz Theisen

    2011-03-01

    Full Text Available Good Governance, Social Market Economy, Culture and Education are the decisive elements for Human Development. We need a third way between the extremes of the Utopian Global Free Market and a new nationalism. A Social Market Economy and the European Model of a Union could be such third way. For a new Social Market Economy we need a renaissance of the European dialectics between culture and society, idealism and materialism, religion and enlightenment, solidarity and profitability. The balancing of those poles is deeply rooted in our best traditions. 

  17. In-depth profiling and analysis of host and viral microRNAs in Japanese flounder (Paralichthys olivaceus) infected with megalocytivirus reveal involvement of microRNAs in host-virus interaction in teleost fish.

    Science.gov (United States)

    Zhang, Bao-cun; Zhang, Jian; Sun, Li

    2014-10-08

    MicroRNAs (miRNAs) regulate gene expression by binding to mRNA transcripts in various biological processes. In mammals and birds, miRNAs are known to play vital parts in both host immune defense and viral infection. However, in lower vertebrates such as teleost, systematic investigations on host and viral miRNAs are lacking. In this study, we applied high-throughput sequencing technology to identify and analyze both host and viral miRNAs in Japanese flounder (Paralichthys olivaceus), an economically important teleost fish farmed widely in the world, infected with megalocytivirus at a timescale of 14 days divided into five different time points. The results showed that a total of 381 host miRNAs and 9 viral miRNAs were identified, the latter being all novel miRNAs that have no homologues in the currently available databases. Of the host miRNAs, 251 have been reported previously in flounder and other species, and 130 were discovered for the first time. The expression levels of 121 host miRNAs were significantly altered at 2 d to 14 d post-viral infection (pi), and these miRNAs were therefore classified as differentially expressed host miRNAs. The expression levels of all 9 viral miRNAs increased from 0 d pi to 10 d pi and then dropped from 10 d pi to 14 d pi. For the 121 differentially expressed host miRNAs and the 9 viral miRNAs, 243 and 48 putative target genes, respectively, were predicted in flounder. GO and KEGG enrichment analysis revealed that the putative target genes of both host and viral miRNAs were grouped mainly into the categories of immune response, signal transduction, and apoptotic process. The results of our study provide the first evidences that indicate existence in teleost fish (i) infection-responsive host and viral miRNAs that exhibit dynamic changes in expression profiles during the course of viral infection, and (ii) potential involvement of miRNAs in host-viral interaction.

  18. Causality in Europeanization Research

    DEFF Research Database (Denmark)

    Lynggaard, Kennet

    2012-01-01

    to develop discursive institutional analytical frameworks and something that comes close to the formulation of hypothesis on the effects of European Union (EU) policies and institutions on domestic change. Even if these efforts so far do not necessarily amount to substantive theories or claims of causality...

  19. Measuring European selves

    DEFF Research Database (Denmark)

    Antalikova, Radka

    Taking the perspective of cross-cultural psychology, the current thesis sets out to investigate self in a European context. For this purpose, the thesis first thoroughly reviews the most prominent conceptualizations of self in cross-cultural psychology, specifically focusing on disentangling...... to the field of cross-cultural psychology, specifically highlighting the utility of autobiographical memories as measures of self....

  20. European Psychology Map

    Directory of Open Access Journals (Sweden)

    DANA SCHÖN

    2009-06-01

    Full Text Available The Institute for Psychology Information (ZPID compiled an extensive list of European psycho-logical organizations, comprised of university departments, research institutions, professional associa-tions and publishing houses. The list is available on the ZPID website, together with a web mapping applet that indicates the exact geographical location of the organizations.

  1. European Pine Shoot Moth

    Science.gov (United States)

    William E. Miller; Arthur R. Hastings; John F. Wootten

    1961-01-01

    In the United States, the European pine shoot moth has caused much damage in young, plantations of red pine. It has been responsible for reduced planting of red pine in many areas. Although attacked trees rarely if ever die, their growth is inhibited and many are, deformed. Scotch pine and Austrian pine (Pinus nigra Arnold) are usually not so badly damaged. Swiss...

  2. Inequalities in European cities

    NARCIS (Netherlands)

    Musterd, S.; Ostendorf, W.; Smith, S.J.; Elsinga, M.; Eng, O.S.; Fox O’Mahony, L.; Wachter, S.

    2012-01-01

    The consequences of inequalities in European cities are a big fear for many governments at the state and urban levels. Journalists, as well as many scholars who are dealing with urban issues, express their fears about the development of social, ethnic, and spatial divisions. Population categories

  3. European 'Stabilisation through Association'

    DEFF Research Database (Denmark)

    Rodt, Annemarie Peen

    ’s success in repeatedly reconciling a divided continent and complemented its efforts to build peace beyond its borders. But does the EU (continue to) deserve such praise? This contribution examines European peacebuilding from the early inte-gration of post-World War Two economies, through the uniting...

  4. European Integration and Globalization

    Directory of Open Access Journals (Sweden)

    Diana Bobica

    2010-06-01

    Full Text Available According to many, the term globalization is able to explain any phenomenon whatsoever, be it positive or negative, that takes place within the global social system. It seems like a sort of magical formula, which is to be found in the speeches of all sorts of people, be they economists, politicians, businessmen or sociologists. However this magical formula of globalization has its limitations, since it encompasses a certain amount of quibbling, beyond which not many can pass. In the context of globalization there appears the question on its role in the process of European integration. Is European integration a part of this global process or, quite on the contrary, does it present certain distinctive features, as it moulds itself differently from the globalization phenomenon? A clear-cut answer seems difficult because of the various aspects involved. Not only the general phenomenon of globalization, but also the economic integration on European level is based on the liberalization of markets and on the opening of national economies towards the exterior,having as direct consequence the intensification of trade exchanges. If from a global point of view one may talk of a market fundamentalism in that the market principles know no boundary, European integration on the other hand implies not only market economy, but also a guided and monitored action of Member Statesaccording to the needs of the whole entity, also taking into consideration - as far as possible – all aspects and consequences on social level.

  5. Becoming Pan-European?

    DEFF Research Database (Denmark)

    Schulz-Forberg, Hagen; Brüggemann, Michael

    2009-01-01

    be platforms of a transnational European discourse. Four ideal-types of transnational media can be distinguished: (1) national media with a transnational mission, (2) international media, (3) pan-regional media and (4) global media. Within this framework the article analyses transnational media in Europe...

  6. European Metals Conference

    CERN Document Server

    Vereecken, Jean

    1991-01-01

    This volume contains the papers that will be presented at 'EMC '91 '-the European Metals Conference-to be held in Brussels, Belgium, from 15 to 20 September 1991, and organized by Benelux Metallurgie, GDMB (Gesellschaft Deutscher Metallhutten­ und Bergleute) and IMM (the Institution of Mining and Metallurgy). 'EMC '91' is the first of an intended major series organized at the European level with the aim of bringing together all those who are involved with the extraction and processing of non-ferrous metals-European metallurgists and their international colleagues-to provide them with the opportunity to exchange views on the state and evolution of their industry. The programme covers all the different aspects of the metallurgy of non-ferrous metals from mining to fabricated products. Particular attention is being paid to the European non -ferrous industry with respect to changes in demand, the technology used, pressures on the environment and the competitive position of manufacturers. The contributions of the...

  7. AND THE EUROPEAN UNION

    African Journals Online (AJOL)

    Regulations governing the production and use of genetically modified organisms have been developed in the United Kingdom since 1976. Regulations covering the release of transgenic organisms into the environment were initially voluntary. Since 1990, the European Economic Commission (EEC) Directive. 90/219 and ...

  8. European Music Year 1985.

    Science.gov (United States)

    Alexanderson, Thomas; And Others

    1984-01-01

    Articles concerning music are included in this newsletter dedicated to cultural venture to be jointly carried out by the Council of Europe and the European communities. Many events will mark Music Year 1985, including concerts, dance performances, operas, publications, recordings, festivals, exhibitions, competitions, and conferences on musical…

  9. The Europeanness of European cinema: Identity, meaning, globalization

    OpenAIRE

    Willems, Gertjan

    2015-01-01

    According to its editors, the aim of The Europeanness of European cinema is ‘to revisit the issue of the significance of European cinema as a category in the wake of the recent acceleration in transnational filmmaking and globalization as a whole’ (p. 7). Taking the transnational prominence of European cinema as a starting point, the anthology’s introduction presents some brief reflections about what ‘Europeanness’ – the central, overarching concept for the various chapters – has meant histor...

  10. Molecular cloning, structure and expressional profiles of two novel single-exon genes (PoCCR6A and PoCCR6B) in the Japanese flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Wang, Lei; Zhang, Yong-zhen; Xu, Wen-teng; Jia, Xiao-dong; Chen, Song-lin

    2016-05-01

    CCR6 is an important binding receptor of CCL20 and beta-defensins, and has multiple functions in the innate and acquired immune responses. In this study, we cloned the PoCCR6A and PoCCR6B genes of the Japanese flounder and studied the gene structure and expression patterns of these two genes in bacterial infection. The full-length PoCCR6A cDNA is 1415 bp and the open reading frame (ORF) is 1113 bp, encoding a 370-amino-acid peptide. The full-length PoCCR6B cDNA is 2193 bp and the ORF is 1029 bp, encoding a 363-amino-acid peptide. The structures of PoCCR6A and PoCCR6B indicate that they are single-exon genes. The predicted proteins encoded by PoCCR6A and PoCCR6B have the typical G-protein-coupled receptor (GPCR) family signature of seven transmembrane domains and several conserved structural features. A tissue distribution analysis showed that PoCCR6A is predominately expressed in the intestine, gill, and blood, and PoCCR6B in the gill, spleen, and liver. The expression patterns of the two chemokine receptors were analyzed during bacterial infection. In spleen and kidney, the expression of PoCCR6A was significantly upregulated at 24 h after infection, whereas the expression of PoCCR6B was steady at these time points. While in intestine, both of them were upregulated at 6 h-12 h after infection, and in gill the expression levels of them were upregulated at 24 h. The patterns of expression suggested that PoCCR6A and PoCCR6B play an important role in the immune response of the Japanese flounder, especially in the mucosal tissues. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Revisión sobre aspectos biológicos y de cultivo del lenguado chileno (Paralichthys adspersus Review of the biology and cultivation of Chilean flounder (Paralichthys adspersus

    Directory of Open Access Journals (Sweden)

    Alfonso Silva

    2010-01-01

    Full Text Available El lenguado chileno (Paralichthys adspersus es una especie endémica de la costa chilena, que ha sido objeto de diversos estudios tendientes a analizar las posibilidades que presenta para diversificar la acuicultura marina. El presente trabajo hace una revisión de los diversos resultados obtenidos en el estudio de su biología y técnicas de cultivo, observándose que existe el conocimiento zootécnico necesario para desarrollar su ciclo biológico completo en cautividad y producir individuos de tamaño comercial en condiciones de cultivo. Sin embargo, este conocimiento se encuentra disperso y aún muestra comparativamente con otras especies cultivables, una moderada replicabilidad de los resultados, lo que implica la necesidad de persistir en la optimización de algunos aspectos de su cultivo, para impulsar definitivamente su desarrollo.Chilean flounder (Paralichthys adspersus are endemic to the Chilean coast and have been the subject of several studies on the possible diversification of marine aquaculture. This paper reviews the diverse results obtained from studies of this species' biology and cultivation techniques. The biological and technical knowledge available is sufficient for developing the entire life cycle of this species, producing commercial-sized individuals, in captivity. However, this knowledge is disperse and the results, as compared with those for other harvested species, still show moderately reproducible results, implying the need to continue efforts to optimize some aspects of Chilean flounder cultivation, thereby promoting the species' development definitely.

  12. European core curriculum in neurorehabilitation

    NARCIS (Netherlands)

    Sandrini, G.; Binder, H.; Homberg, V.; Saltuari, L.; Tarkka, I.; Smania, N.; Corradini, C.; Giustini, A.; Katterer, C.; Picari, L.; Diserens, K.; Koenig, E.; Geurts, A.C.; Anghelescu, A.; Opara, J.; Tonin, P.; Kwakkel, G.; Golyk, V.; Onose, G.; Perennou, D.; Picelli, A.

    2017-01-01

    To date, medical education lacks Europe-wide standards on neurorehabilitation. To address this, the European Federation of NeuroRehabilitation Societies (EFNR) here proposes a postgraduate neurorehabilitation training scheme. In particular, the European medical core curriculum in neurorehabilitation

  13. European and Integration Studies

    Directory of Open Access Journals (Sweden)

    N. Yu. Kaveshnikov

    2014-01-01

    Full Text Available Soviet scientific school of pan-European integration studies began to emerge in the 1960s at the Institute of World Economy and International Relations (Russian Academy of Science. Among the leading scientists who have developed methodological approaches of Soviet integration studies were M.M. Maximova, Y.A. Borko, Y. Shishkov, L.I. Capercaillie. Later, a new center for integration studies became the Institute of Europe, created in 1987. It was led by such renowned scientists as Academicians V.V. Zhurkin and N.P. Shmelev. In the 1980s the subject of the integration process in Europe attracted attention of experts from MGIMO. An important role in the development of school of integration research in the USSR was played by a MGIMO professor, head of the chair of history of international relations and foreign policy of the USSR V.B. Knyazhinskiy. His work contributed to the deliverance of the national scientific community from skepticism about the prospects for European integration. Ideas of V.B. Knyazhinsky are developed today in MGIMO by his followers A.V. Mal'gin and T.V. Ur'eva. In the mid-1990s, having retired from diplomatic service, professor Yu. Matveevskiy started to work at MGIMO. With a considerable practical experience in the field, he produced a series of monographs on the history of European integration. In his works, he analyses the development of integration processes in Western Europe from their inception to the present day, showing the gradual maturation of the necessary spiritual and material prerequisites for the start of integration and traces the various stages of the "integration". In the late 1990s, the growing demand from the domestic business and government for professionals who are capable of interacting with the European Union, has produced the necessary supply in the form of educational programs based on accumulated scientific knowledge. Setting up a discipline "European Integration" was a major step in the development

  14. The Shoah within European identity

    NARCIS (Netherlands)

    van der Poel, Stefan

    2016-01-01

    In the Epilogue of Postwar, titled: From the House ofthe Dead: An essay on Modern European memory, Tony Judt concludes that: ‘those who would become full Europeans in the dawn of the twenty-first century must first assume a new and far more oppressive heritage. Today the pertinent European reference

  15. Future European biogas

    DEFF Research Database (Denmark)

    Meyer, A. K.P.; Ehimen, E. A.; Holm-Nielsen, J. B.

    2017-01-01

    Biogas is expected to play an important role in reaching the future energy policy targets of the European Union (EU). The sustainability of biogas substrates has however been recently critically discussed due to the increasing shares of agricultural land used for energy crop production.The aim...... were animal manure, straw by-products from cereal production, and excess grass from rotational and permanent grasslands and meadows. The biogas energy potential from the investigated biomass was projected to range from 1.2·103 to 2.3·103 PJ y-1 in year 2030 in the EU28, depending on the biomass...... availability. Alone the biogas energy potential projected in the scenario representing low substrate availability corresponds to a doubling of the European biogas production in 2015. The results shows that sustainable alternatives to the use of maize are present in all the member states of the EU28...

  16. European Patterns of Participation

    DEFF Research Database (Denmark)

    Harrebye, Silas Fehmerling; Ejrnæs, Anders

    2015-01-01

    Social Survey (ESS) Round 4 (2008), the article finds that satisfaction/dissatisfaction with the government is an important predictor alongside the institutional macro-level variable. The article combines a critical tradition, which suggests that political participation is motivated by a feeling...... of dissatisfaction with the government and feelings of being member of a discriminated group affect the level of extra-parliamentary participation, and second, how different welfare regimes condition the extend to which these groups chose to act. In a comparative multilevel design, using data from the European...... of dissatisfaction with an institutional perspective in which certain institutional conditions are seen as enablers for citizens to actively participate in political life. Our results show that the overall level of extra-parliamentary activity in the Scandinavian countries is higher than in the other European...

  17. Chestnut, European (Castanea sativa).

    Science.gov (United States)

    Corredoira, Elena; Valladares, Silvia; Vieitez, Ana M; Ballester, Antonio

    2015-01-01

    Development of a system for direct transfer of antifungal candidate genes into European chestnut (Castanea sativa) would provide an alternative approach to conventional breeding for production of chestnut trees that are tolerant to ink disease caused by Phytophthora spp. Overexpression of genes encoding PR proteins (such as thaumatin-like proteins), which display antifungal activity, may represent an important advance in control of the disease. We have used a chestnut thaumatin-like protein gene (CsTL1) isolated from European chestnut cotyledons and have achieved overexpression of the gene in chestnut somatic embryogenic lines used as target material. We have also acclimatized the transgenic plants and grown them on in the greenhouse. Here, we describe the various steps of the process, from the induction of somatic embryogenesis to the production of transgenic plants.

  18. Do Europeans Like Nudges?

    DEFF Research Database (Denmark)

    Reisch, Lucia A.; Sunstein, Cass R.

    2016-01-01

    In recent years, many governments have shown a keen interest in “nudges” — approaches to law and policy that maintain freedom of choice, but that steer people in certain directions. Yet to date, there has been little evidence on whether citizens of various societies support nudges and nudging. We...... report the results of nationally representative surveys in six European nations: Denmark, France, Germany, Hungary, Italy, and the United Kingdom. We find strong majority support for nudges of the sort that have been adopted, or under serious consideration, in democratic nations. Despite the general...... European consensus, we find markedly lower levels of support for nudges in two nations: Hungary and Denmark. We are not, in general, able to connect support for nudges with distinct party affiliations....

  19. Pirating European Studies

    Directory of Open Access Journals (Sweden)

    Natalia Timus

    2016-08-01

    Full Text Available Open Science has gained a lot of attention not only within the academic community but also among policy-makers. Some international publishers have been active in moving towards open access publications and research data, but, overall, modest results have been achieved so far. In this context, the digital piracy engines emerge as vital actors in disseminating and determining the impact of research. This study examines the Sci-Hub downloads data in order to uncover patterns of piracy in European Studies research. We identify journals and the subjects of articles that have been pirated the most. We also study the geographical distribution of download requests. The analysis reveals that the readers are mostly interested in subjects reflecting the current major European challenges, specifically populism and the economic crisis. Both developing countries as well as the ‘old’ EU members are active in illegal downloads.

  20. Stature of early Europeans.

    Science.gov (United States)

    Hermanussen, Michael

    2003-01-01

    The ancestors of modern Europeans arrived in Europe at least 40,000 years before present. Pre-glacial maximum Upper Palaeolithic males (before 16,000 BC) were tall and slim (mean height 179 cm, estimated average body weight 67 kg), while the females were comparably small and robust (mean height 158 cm, estimated average body weight 54 kg). Late Upper Palaeolithic males (8000-6600 BC) were of medium stature and robusticity (mean height 166 cm, estimated average body weight 62 kg). Stature further decreased to below 165 cm with estimated average body weight of 64 kg in Neolithic males of the Linear Band Pottery Culture, and to 150 cm with estimated average body weight of 49 kg in Neolithic females. The body stature of European males remained within the range of 165 to 170 cm up to the end of the 19th century.

  1. European Corporate Law

    DEFF Research Database (Denmark)

    Werlauff, Erik; Dorresteijn, Adriaan; Teichmann, Christoph

    This fully updated new edition provides the best-known practical overview of the law regarding companies, business activities, and capital markets in Europe, at both the European Union (EU) and Member State levels. It incorporates analysis of recent developments including the impact of global......; - a company’s freedom to incorporate in a jurisdiction not its own; - competition among the legal forms of different Member States; and - safeguarding of employee involvement in cross-border transactions. With respect to national law, the laws of Belgium, France, Germany, the Netherlands, Poland, Spain...... initiatives in such aspects of the corporate environment as regulation of financial institutions and non-financial reporting obligations with a view to sustainability and other social responsibility concerns. The authors, all leading experts in European corporate law, describe current and emerging trends...

  2. Telemedicine and European law.

    Science.gov (United States)

    Callens, Stefaan

    2003-01-01

    A Directive of the European Union was first published in 2000, which dealt with telemedicine as part of its provisions. This E-Commerce Directive, as it became known, was subjected to further study which revealed some problems relative to the practice of telemedicine. Among the subjects discussed in this paper are those of privacy, data protection, free movement of services, the impact of electronic communication and ethical issues.

  3. European Automotive Congress

    CERN Document Server

    Clenci, Adrian

    2016-01-01

    The volume includes selected and reviewed papers from the European Automotive Congress held in Bucharest, Romania, in November 2015. Authors are experts from research, industry and universities coming from 14 countries worldwide. The papers are covering the latest developments in fuel economy and environment, automotive safety and comfort, automotive reliability and maintenance, new materials and technologies, traffic and road transport systems, advanced engineering methods and tools, as well as advanced powertrains and hybrid and electric drives.

  4. Geography of European Migration

    Directory of Open Access Journals (Sweden)

    Zhitin Dmitry V.

    2016-08-01

    Full Text Available In recent decades, the role of international migration has increased dramatically in most European countries. The growth in migration has made some authors proclaim the beginning of a second Migration Period that could transform the social and cultural identity of Europe. The article presents an analysis of international migration geography in Europe in the last twenty-five years. The authors identify the main trends in migration, provide migration profiles of European countries, and propose a classification based on the recent changes in the migrant stock. Changes in the migrant stock (total emigration and immigration reflect the level of involvement in international and global processes. They can serve as an indicator of a country’s attractiveness for both foreigners and the country’s citizens. The study shows that European countries are increasingly split into ‘immigrant’ and ‘emigrant’ states. The authors describe spatial patterns of migration. The volume and localisation of migration flows in Europe are affected not only by cultural and historical circumstance, such as a colonial past or a common language. The scale of immigrant influx often does not depend on a donor country’s demographic potential or the level of its socio-economic development. The links between the place of origin and destination are often more complex than it might initially seem. The authors stress the importance of a differentiated immigration policy taking into account ethnic and cultural features of host societies.

  5. European Law in the Making:

    DEFF Research Database (Denmark)

    Rasmussen, Morten

    the legal service of the High Authority from early on promoted a ‘constitutional’ interpretation of European law, but where the member state, as well as most legal experts, still considered European law a subset of international law. How did the Court of Justice of the ECSC manoeuvre between...... on European law in the making.......Traditionally, the Court of Justice of the European Coal and Steel Community (ECSC) has mainly been considered the somewhat dull predecessor to the more famous Court of Justice of the European Communities, which in 1963-64 ‘constitutionalised’ the Treaties of Rome with the seminal judgments of Van...

  6. Development of water purification technologies for intensive fish culture. 10. Rearing of Japanese flounder with closed seawater recirculation system equipped with immobilized bacteria; Konoritsu gyorui seisan no tame no suishitsu joka gijutsu no kaihatsu. 10. Hirame shiiku eno koteika biseibutsu no tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Uemoto, H.; Watanabe, Y.; Kikuchi, K.

    1994-03-01

    Japanese flounder were reared by a seawater recirculation and filtration system with immobilized bacteria. As a water purification system, used were a nitrification tank and denitrification tank which were both filled with immobilized nitrifying bacteria and denitrifying bacteria, respectively. The flounder could be reared for 180 days with their body weight doubled at the end of experiment. Ammonia exhausted from the flounder was not accumulated in the rearing water. Nitric acid produced by the oxidation of ammonia was prevented from heightening its concentration in the denitrification tank. Usable immediately after having been filled into the filtration tank, both immobilized nitrifying and denitrifying bacteria are stable in activity without being affected by organic matter in the rearing water. The dissolution and disintegration of carrier were not confirmed. The high activity of immobilized nitrifying bacteria and denitrifying bacteria was observed on the surface and in the central part, respectively of the carrier. The activity of both bacteria was confirmed inside the carrier. From the foregoing, the high density rearing was known to be possible even in a filtration tank miniaturized to the size of about 20% of the conventional one. 29 refs., 18 figs., 2 tabs.

  7. Eastern Dimension of the European Neighbourhood Policy: Europeanization Mutual Trap

    Directory of Open Access Journals (Sweden)

    V. A. Latkina

    2014-01-01

    Full Text Available The article analyses the Europeanization policy of the European Union towards the Eastern Partnership participant countries. Suffering from the lack of clear strategy and ultimate goal in the European Neighbourhood Policy the European Union enhances external democratization and its governance in post soviet states without immediate Union's membership perspective. Underestimation of common neighbourhood geopolitical duality in the context of growing rivalry between European (EU and Eurasian (Custom Union/Eurasian Economic Union integration gravitation centers presents the Eastern partners of the EU with a fierce dilemma of externally forced immediate geopolitical and civilizational choice while not all of them are well prepared to such a choice. The mutual Europeanization trap here to be studied both for the EU and its Eastern partners (involving Russia is a deficiency of regulating cooperation mechanism in the situation of European and Eurasian free trades zones overlapping. Vilnius Summit 2013 results test the "European aspirations" of the New Independent States and upset the ongoing process of the European Neighbourhood Policy in the context of growing economic interdependence in Wider Europe. Besides, the Ukrainian crisis escalation during 2014 as a new seat of tension provokes unbalance of the whole European security system and creates new dividing lines in Europe from Vancouver to Vladivostok.

  8. Cortisol and melatonin in the cutaneous stress response system of fish.

    Science.gov (United States)

    Kulczykowska, Ewa; Kalamarz-Kubiak, Hanna; Gozdowska, Magdalena; Sokołowska, Ewa

    2018-02-01

    The stress hormone cortisol, together with antioxidants, melatonin (Mel) and its biologically active metabolites, 5-methoxykynuramines, including AFMK, set up a local stress response system in mammalian skin. Our in vitro study of the European flounder (Platichthys flesus) was designed to examine whether Mel and AFMK would respond to cortisol while a glucocorticoid is added to the incubation medium. The concentrations of cortisol in the incubation medium mimic plasma cortisol levels seen in fish exposed to different types of stresses such as handling, confinement, high density, food-deprivation or air-exposure. We measured Mel and AFMK in skin explants and culture media using high-performance liquid chromatography (HPLC) with fluorescence detection. We also analysed melanosome response (dispersion/aggregation) in the explants subjected to the different treatments. Cortisol stimulated the release of Mel and AFMK from skin explants in a dose-dependent manner. Melanosome dispersion and a darkening of the skin explants were observed after incubation with cortisol. This study is the first to demonstrate the interrelationship between cortisol and Mel/AFMK in fish skin. Our data strongly suggest that the cutaneous stress response system (CSRS) is present in fish. The question remains whether Mel, AFMK or cortisol are synthetized locally in fish skin and/or transported by the bloodstream. The presence of the CSRS should be taken into account during elaboration of new indicators of fish welfare both in aquaculture and in the wild. Copyright © 2018 Elsevier Inc. All rights reserved.

  9. The "bioeffect assessment index" (BAI). A concept for the quantification of effects of marine pollution by an integrated biomarker approach.

    Science.gov (United States)

    Broeg, K; Westernhagen, H V; Zander, S; Körting, W; Koehler, A

    2005-05-01

    The "bioeffect assessment index" (BAI) is based on the integration of several pathological endpoints measured in the liver of European flounder (Platichthys flesus (L.)) during a long term study of biological effects of pollution in the German Bight. The BAI represents a modification of the "health assessment index" since it includes solely validated biomarkers reflecting toxically induced alterations at different levels of biological organisation in order to quantify the effects of environmental pollution. The concept of the BAI is based on the observation of progressive deleterious effects from early responses to late effects. Specific "key events" were detected, representing progressive stages of functional deterioration. The biomarkers selected from a whole battery of cellular markers for the BAI calculation reflect deleterious effects of various classes of contaminants such as heavy metals, organochlorines, pesticides, PAHs, and therefore reflect general toxicity in an integrative manner. Selected biomarkers were: lysosomal perturbations (reduced membrane stability), storage disorders (lipid accumulation) as early markers for toxic effects of liver cells, and the size of macrophage aggregates and their acid phosphatase activity. The latter two markers are indicative for the modulation of non-specific immune response which represents longer time scale responses after chronic exposure.

  10. The European populist challenge

    Directory of Open Access Journals (Sweden)

    Yannis Stavrakakis

    2013-01-01

    Full Text Available In today’s Europe, the word ‘populism’ usually refers to right-wing populism or the populist extreme right. Is, however, the concept of ‘populism’ the proper theoreticopolitical instrument through which such identifications should be perceived, categorized and debated? What are the implications (direct and indirect of such a naming? And what are the risks for critical analysis and for democratic politics in the European context? The hypothesis explored in this essay is that sticking to a restrictive association between ‘populism’ and the extreme right poses certain dangers that have to be seriously taken into account, especially in times of crisis. For a start, it is often premised on a rather simplistic euro-centrism that reduces the broad conceptual spectrum covered by the category ‘populism’ in its global use to a very particular European experience and then essentializes the resulting association, over-extending its scope. In addition, the category ‘populism’ is aften used to describe political forces, identities and discourses in which the role of ‘the people’ is only secondary or peripheral, to the extent that it has to coincide with strongly hierarchical and elitist visions of society. What complicates things even further is that, within the context of the European (economic and political crisis, whoever questions/ resists the austerity agenda – especially on the left – is increasingly discredited and denounced as an irresponsible populist. Indeed, it is not by coincidence that doubts are increasingly voiced both in the theoretical and in the political literature regarding the rationale behind such a strong association between populism and the extreme right. A series of points will thus be raised that may help us develop a plausible theoretico-political strategy in the new emerging conditions from a discursive perspective.*

  11. European Network Against Racism

    DEFF Research Database (Denmark)

    Nielsen, Helene Pristed

    of discussions about intersectionality, the article investigates ENAR´s attention and ability to integrate questions of e.g. gender, sexuality or age in their policy input, given the potentially rigid compartmentalisation of discrimination strands within the European Commission´s system of civil society...... consultations. The article therefore overall aims to assess ENAR´s position as potentially hemmed in between 1) the Commission vs. member organisations’ expectations for its focus and modus operandi and 2) attention to intersectional discrimination vs. potential compartmentalisation within the Commission...

  12. Cooperative strategies European perspectives

    CERN Document Server

    Killing, J Peter

    1997-01-01

    Cooperative Strategies: European Perspectives is one of three geographically targeted volumes in which the contributors present the most current research on topics such as advances in theories of cooperative strategies, the formation of cooperative alliances, the dynamics of partner relationships, and the role of information and knowledge in cooperative alliances. Blending conceptual insights with empirical analyses, the contributors highlight commonalities and differences across national, cultural, and trade zones. The chapters in this volume are anchored in a wide set of theoretical approaches, conceptual frameworks, and models, illustrating how rich the area of cooperative strategies is for scholarly inquiry.

  13. European Regional Modernism

    Directory of Open Access Journals (Sweden)

    Vincent Brian Canizaro

    2014-03-01

    Full Text Available In recent years, beginning with the publication in 2003 of Liane Lefaivre and Alexander Tzonis’ 'Critical Regionalism', followed by my 'Architectural Regionalism: Collected Writings on Place, Identity, Modernity and Tradition 'in 2007, there has been a quiet resurgence in the discourse of architectural regionalism.' 'Leuven University Press’s 'Regionalism and Modernity: Architecture in Western Europe 1914–1940 'continues in this direction, with eleven chapters devoted to variations of the regionalist tendency in European architecture focused primarily on Belgium and France, but also Great Britain, Italy, and Germany.

  14. European Union of Memories?

    DEFF Research Database (Denmark)

    Wæhrens, Anne

    After a very brief introduction to history and memory in Europe after 1989, as seen by Aleida Assmann, I will give a short introduction to the EP and to their adoption of resolutions and declarations. Then I will define some concepts central to my study before I proceed to the analysis. Finally I...... these changes have come about. Moreover, I show that there seems to be a political memory split between Left and Right and I suggest that the time might not be ripe for a shared European memory....

  15. European Union Budget Politics

    DEFF Research Database (Denmark)

    Citi, Manuele

    2015-01-01

    The marginal involvement of the European Union (EU) in redistributive policies and its limited fiscal resources have led to a notable lack of attention by EU scholars towards the EU budget and its dynamics. Yet the nature of the budgetary data and their high usability for statistical analysis make...... them an excellent tool for studying and measuring policy change in the EU. In this article, I analyse an original dataset containing yearly data for the main categories of expenditure and how they have changed over the last three decades (1979–2013). Using time-series analysis, I find that the ability...

  16. How campaigns enhance European issues voting during European Parliament elections

    DEFF Research Database (Denmark)

    Beach, Derek; Møller Hansen, Kasper; Larsen, Martin Vinæs

    2017-01-01

    allegiances less important and attitudes about the European project more important by informing voters of and getting them interested in European politics. In effect, we argue that the political campaign leading up to the election makes European Parliament elections less second-order. While previous studies...... have demonstrated that EU attitudes can matter for voting behavior in European Parliament elections, existing research has drawn on post-election surveys that do not enable us to capture campaign effects. Our contribution is to assess the impact of a campaign by utilizing a rolling cross sectional......Based on findings from the literature on campaign effects on the one hand, and the literature on European Parliament elections on the other, we propose a model of European Parliamentary elections in which the campaign shift the calculus of electoral support, making differences in national political...

  17. Europeanness: A Path To Unity Within The European Union

    Directory of Open Access Journals (Sweden)

    Piasecki Ryszard

    2016-03-01

    Full Text Available The postwar experience has shown that the implanting of European consciousness, or Europeanness, calls for coordinated efforts among the European institutions, national states, and NGOs. Such consciousness, a key pillar of the European integration, is necessary for the EU to effectively function and motivate member states’ − also the EU’s − citizens. And yet European institutions and EU governments show little interest in promoting the formation of this European consciousness. Pro-European social movements are weak, while anti- European ones gain strength. This désintéressement of the EU countries probably results from the conviction that the goal has been reached and that there is no more need for a widespread pro-European education of their societies. However analysis of the problem, and in particular of the interaction between European and national identities, shows that this is not the case. We fear that this lack of proactive measures mobilizing EU citizens to keep on struggling for a common Europe will lead to the erosion of existing achievements of integration within the EU, and undermine European values. It may threaten the future of the EU, which is not an ordinary integration grouping but a great peaceful, civilizational, social and economic project. Our hypothesis − positively verified in this article − is that the promotion of Europeanness in the EU societies is urgently needed to maintain the unity (and even membership of the Union, and to avert trends unfavorable for all of Europe and therefore for the West as a whole.

  18. Floating along buoyancy levels: dispersal and survival of western Baltic fish eggs

    DEFF Research Database (Denmark)

    Petereit, C.; Hinrichsen, H.-H.; Franke, A.

    2014-01-01

    Vertical distribution is an important feature of pelagic fish eggs and yolk sac larvae impacting their survival and dispersal, especially in heterogeneous and highly variable estuarine environments like the Baltic Sea. Egg densities determining the vertical distribution pattern were experimentally...... ascertained for cod (Gadus morhua), plaice (Pleuronectes platessa) and flounder (Platichthys flesus) from the western Baltic Sea. Plaice eggs floated at lower mean (± standard deviation) density range (1.0136 ± 0.0007 g cm-3) compared to cod (1.0146 ± 0.0009 g cm-3) and flounder eggs (1.0160 ± 0.0015 g cm-3......), which floated on the highest density level. In flounder egg diameter was significantly related to egg density and in cod a weak correlation could be found between egg dry weight and density. All other relationships between female size, egg size, egg dry weight and egg density were not significant...

  19. The European Social Survey and European research policy

    DEFF Research Database (Denmark)

    Kropp, Kristoffer

    2017-01-01

    This article analyses the history of the European Social Survey (ESS) and its relationship to changes in European research policy, using Bourdieu’s field-analytical approach. It argues that the success of the ESS relied on three interwoven processes that we can understand theoretically in terms o...

  20. Can the European Central Bank Create a European identity?

    DEFF Research Database (Denmark)

    Ravn Sørensen, Anders

    2014-01-01

    In what ways do central banks construct community, and how may the European Central Bank (ECB) contribute to a supranational European identity? In this paper I seek to answer these two questions by developing a conceptual framework for the ways that central banks construct national identities and...

  1. Rectors of European universities

    CERN Multimedia

    Maximilien Brice

    2003-01-01

    Several rectors of European universities visited CERN recently while in Geneva for a conference on coordination between their institutions. The visit began with a welcome by Roger Cashmore, CERN Director of Collider Programmes,and continued with tours of CMS, ALICE and the LHC magnet assembly hall. Photos 01, 02: The visitors in the ALICE assembly hall: (left to right) Dr. Raymond Werlen, Deputy Secretary-General of the Conference of Rectors of Swiss Universities; visit guide Prof. Alain Blondel, Department of Nuclear and Corpuscular Physics, University of Geneva; Prof. Adriano Pimpão, Rector of the University of Algarve, President of the Council of Rectors of Portuguese Universities; Prof. Jean-Pierre Finance, Conference of University Presidents, France; Prof. Jean-Paul Lehners, Vice-President of the Centre Universitaire, Luxemburg.

  2. Characterizing European cultural landscapes

    DEFF Research Database (Denmark)

    Tieskens, Koen F.; Schulp, Catharina J E; Levers, Christian

    2017-01-01

    Almost all rural areas in Europe have been shaped or altered by humans and can be considered cultural landscapes, many of which now are considered to entail valuable cultural heritage. Current dynamics in land management have put cultural landscapes under a huge pressure of agricultural...... intensification and land abandonment. To prevent the loss of cultural landscapes, knowledge on the location of different types of cultural landscapes is needed. In this paper, we present a characterization of European cultural landscapes based on the prevalence of three key dimensions of cultural landscapes......: landscape structure, management intensity, and value and meaning. We mapped these dimensions across Europe at a 1-km resolution by combining proxies on management intensity and landscape structure with new indicators such as social media usage and registered traditional food products. We integrated...

  3. European project ISAWARE

    Science.gov (United States)

    Kaiser, Jochen; Smietanski, Guillaume; Kubbat, Wolfgang

    2001-08-01

    As air traffic is increasing, the probability of encountering 'surveillance' alerts during flight is also increasing. In order to ensure safety, new on board systems need to be developed to provide the crew with a better 'situation awareness' (SA) about its external environment and potential hazards. In addition, the means to manage the data generated by these new systems needs to be build up. Despite the tremendous amount of information, crew workload must not increase. This is where the ISAWARE project comes in with the Integrated Situation Awareness System (ISAS) concept. ISAWARE (Increasing Safety through collision Avoidance WARning intEgration) is a project partly funded by the European Community, executed by a well balanced composition of several European aerospace companies (airframers, a helicopter manufacturer, avionics suppliers, airlines), one research laboratory and one university. The overall objective of the ISAWARE project is to conduct research into the potential improvements to flight safety that can be achieved by providing the pilot with complete predictive situation awareness during all phases of the flight. The Integrated Situational Awareness System (ISAS) merges data from different safety systems concerning terrain, traffic, weather and other. The system ensures the alerts consistency, prioritises alerts and anticipates threats along a predicted trajectory earlier than current systems can provide. The second main axis of the research is the development of synthetic vision displays (PFD, ND and HUD) to enhance the Human-Machine Interface (HMI). The key focus of the project is the development of a ground-based demonstrator rig which is interfaced to a dynamic flight simulator. This rig is used for the evaluation of the ISAWARE concept by a representative range of active airline crews.

  4. A toolbox for European judges

    NARCIS (Netherlands)

    Hesselink, M.W.

    2011-01-01

    The forthcoming instrument on European contract law, be it in the shape of an optional code for cross-border contracts or as an official toolbox for the European legislator, is likely to have a spill-over effect on private law adjudication in Europe. Judges will have no great difficulty in finding

  5. Getting European universities into shape

    NARCIS (Netherlands)

    Jacobs, B.; van der Ploeg, F.

    2006-01-01

    Most European universities lag behind the best universities in the Anglo-Saxon world. A key challenge is to raise resources per student in Europe to US levels. The Lisbon agenda demands fundamental reform of the European university system in order to enhance efficiency, yet avoid grade inflation, to

  6. European Industry, 1700-1870

    NARCIS (Netherlands)

    Broadberry, Stephen; Fremdling, Rainer; Solar, Peter M.

    2008-01-01

    This paper offers an overview of the development of European industry between 1700 and 1870, drawing in particular on the recent literature that has emerged following the formation of the European Historical Economics Society in 1991. The approach thus makes use of economic analysis and quantitative

  7. Developments in European Civil Procedures

    NARCIS (Netherlands)

    Cortés, P.; Mańko, R.; Cortés, P.

    2016-01-01

    This chapter is structured as follows. In Section B we explore the legal basis for the creation of European civil procedures and Europeanization of procedural private law in general. In Section C we provide an overview of existing EU instruments on civil procedure, which we propose to divide into

  8. Third European Stroke Science Workshop

    NARCIS (Netherlands)

    Dichgans, Martin; Planas, Anna M.; Biessels, Geert Jan|info:eu-repo/dai/nl/165576367; van der Worp, Bart|info:eu-repo/dai/nl/189855010; Sudlow, Cathie; Norrving, B.; Lees, Kennedy; Mattle, Heinrich P.

    2016-01-01

    Lake Eibsee, Garmisch-Partenkirchen, November 19 to 21, 2015: The European Stroke Organization convened >120 stroke experts from 27 countries to discuss latest results and hot topics in clinical, translational, and basic stroke research. Since its inception in 2011, the European Stroke Science

  9. Carbon accumulation in European forests

    NARCIS (Netherlands)

    Ciais, P.; Schelhaas, M.J.; Zaehle, S.; Piao, S.L.; Cescatti, A.; Liski, J.; Luyssaert, S.; Le-Maire, G.; Schulze, E.D.; Bouriaud, O.; Freibauer, A.; Valentini, R.; Nabuurs, G.J.

    2008-01-01

    European forests are intensively exploited for wood products, yet they also form a sink for carbon. European forest inventories, available for the past 50 years, can be combined with timber harvest statistics to assess changes in this carbon sink. Analysis of these data sets between 1950 and 2000

  10. Adult Education and European Identity

    Science.gov (United States)

    Negt, Oskar

    2008-01-01

    Europe is coming together. This is a historic project; for the first time in modern history, will and consciousness are used for bringing political, social and cultural unity to the European continent. In this process lifelong learning and hence adult education are gaining in importance. The European project takes place in an age characterised by…

  11. Ranking the European armed forces

    NARCIS (Netherlands)

    Beeres, R.J.M.; Bogers, M.

    2012-01-01

    The degree of collectiveness aimed for in European defence policy raises issues such as burden sharing and relative performance measurement of the European Armed Forces (EAF). This paper compares EAF performance rates on three dimensions: input, throughput and output. In order to express

  12. The Ideology of European Dominance.

    Science.gov (United States)

    Richards, Dona

    1979-01-01

    Describes the evolution of anthropology as a particular manifestation of western European culture. Examines the political implications of the discipline's roots in European intellectual, social, and emotional thought, and discusses the role of Black anthropologists in creating a new anthropology for the redefinition of African peoples. (Author/GC)

  13. The Europeanization of National Judiciaries

    DEFF Research Database (Denmark)

    Jaremba, Urszula; Mayoral, Juan A.

    2018-01-01

    The article is underpinned by the idea that the national courts/judges are expected to act as decentralized European Union judges. However, the general knowledge concerning the impact of EU law on the functioning of national courts as EU judges and the process of Europeanization of national judic...

  14. Action Research in European perspective

    DEFF Research Database (Denmark)

    Rasmussen, Lauge Baungaard

    2004-01-01

    The article gives an overview of how different Italian and Danish contributions to action research can be viewed in an European perspective.......The article gives an overview of how different Italian and Danish contributions to action research can be viewed in an European perspective....

  15. Market forces in european soccer

    NARCIS (Netherlands)

    Haan, M.; Koning, Ruud H.; Witteloostuijn, A. van

    2003-01-01

    Recent decades have witnessed major changes in the market for European soccer. The most profound were the Bosman ruling, which lifted restrictions in the European labor market for soccer talent, and the introduction of the Champions' League, a high-profile international competition that generates

  16. Market forces in european soccer

    NARCIS (Netherlands)

    Haan, M.; Koning, Ruud H.; Witteloostuijn, A. van

    2002-01-01

    Recent decades have witnessed major changes in the market for European soccer. The most profound were the Bosman ruling, which lifted restrictions in the European labor market for soccer talent, and the introduction of the Champions' League, a high-profile international competition that generates

  17. Political Psychology of European Integration

    DEFF Research Database (Denmark)

    Manners, Ian James

    2014-01-01

    The chapter engages in a survey of what political psychology and European integration have to say to each other in the understanding of the European Union. The chapter draws on five strands of political psychology as part of this engagement – conventional psychology, social psychology, social...... construction, psychoanalysis, and critical political psychology. Within each strand a number of examples of scholarship at the interface of political psychology and European integration are examined. The chapter argues that the study of the EU has much to benefit from political psychology in terms of theories...... and methods of European identity and integration, but it also argues that political psychology can benefit from the insights of European integration by rethinking the processes that drive the marking of inside and outside, interior and exterior, belonging and otherness....

  18. Denmark and the European Union

    DEFF Research Database (Denmark)

    Manners, Ian

    2011-01-01

    Over the past two decades Morten Kelstrup’s work has been at the centre of three important intellectual innovations in political science – the study of the EU as a regional political system; European security studies; and small states in European integration. Kelstrup’s best known books (Buzan, K...... of this book, two of Kelstrup’s most important intellectual contributions come from his work on Denmark’s relations with the European Union, and his use of systems theory to understand the EU.......Over the past two decades Morten Kelstrup’s work has been at the centre of three important intellectual innovations in political science – the study of the EU as a regional political system; European security studies; and small states in European integration. Kelstrup’s best known books (Buzan...

  19. HARMONIZED EUROPE OR EUROPEAN HARMONY?

    Directory of Open Access Journals (Sweden)

    Cosmin Marinescu

    2007-07-01

    Full Text Available Recent evolutions in Europe raise questions on the viability of the present economic and social model that defines the European construction project. In this paper, the author will try to explain the viability of institutional European model that sticks between free market mechanisms and protectionism. The main challenge for the EU is about the possibility to bring together the institutional convergence and the welfare for all Europeans. This is the result of the view, still dominant, of European politics elite, according to which institutional harmonization is the solution of a more dynamic and prosper Europe. But, economic realities convince us that, more and more, a harmonized, standardized Europe is not necessarily identical with a Europe of harmony and social cooperation. If „development through integration” seems to be harmonization through „institutional transplant”, how could then be the European model one sufficiently wide open to market, which creates the prosperity so long waited for by new member countries?

  20. European environmental stratifications and typologies

    DEFF Research Database (Denmark)

    Hazeu, G.W,; Metzger, M.J.; Mücher, C.A.

    2011-01-01

    A range of new spatial datasets classifying the European environment has been constructed over the last few years. These datasets share the common objective of dividing European environmental gradients into convenient units, within which objects and variables of interest have relatively homogeneous...... characteristics. The stratifications and typologies can be used as a basis for up-scaling, for stratified random sampling of ecological resources, for the representative selection of sites for studies across the continent and for the provision of frameworks for modeling exercises and reporting at the European...... scale. This paper provides an overview of five recent European stratifications and typologies, constructed for contrasting objectives, and differing in spatial and thematic detail. These datasets are: the Environmental Stratification (EnS), the European Landscape Classification (LANMAP), the Spatial...

  1. Road tunnels safety according to European legislation

    Directory of Open Access Journals (Sweden)

    Fedor KÁLLAY

    2008-01-01

    Full Text Available The article deals with safety of European road tunnels in accordance with actual European legislation. Standards and recommendations of European Commission, PIARC and other professional bodies of the European Union define minimal technological requirements for equipment and operation of the tunnels in scope of Trans-European Road Network.

  2. The European XFEL Project

    CERN Document Server

    Trunk, U

    2008-01-01

    The European XFEL project is a 4th generation photon source to be built in Hamburg. Electron bunches, accelerated to 17.5GeV by the XFEL linac, are distributed to three long SASE undulators. There photon pulses with full lateral coherence and wavelengths between 0.1nm and 4.9nm (12.4 keV and 0.8 keV) are generated for three beamlines. It will deliver around 1012photons within each 100 fs pulse, reaching a peak brilliance of 1033photonss−1mm−2mrad−2(0.1%BW)−1. Thus it will offer unprecedented possibilities in photon science research including nano-object imaging and studies (e.g. by coherent X-ray scattering) and ultra fast dynamic analysis of plasma and chemical reactions (e.g. by X-ray photo correlation spectroscopy). The detector requirements for such studies are extremely challenging: position sensitive area detectors have to provide a dynamic range of ≥ 104, with single-photon sensitivity, while withstanding radiation doses up to 1GGy (TID). Furthermore the detectors have to record data from tra...

  3. Experimental Scuticociliatosis in Japanese flounder (Paralichthys olivaceus) infected with Miamiensis avidus: pathological study on the possible neural routes of invasion and dissemination of the scuticociliate inside the fish body.

    Science.gov (United States)

    Moustafa, Eman Moustafa Moustafa; Tange, Nahoko; Shimada, Akinori; Morita, Takehito

    2010-12-01

    Japanese flounder (Paralichthys olivaceus) were experimentally infected with the highly pathogenic scuticociliate Miamiensis avidus (syn. Philasterides dicentrarachi) using the immersion method to clarify/identify the possible neural routes of entry and possible ways of dissemination of the scuticociliate in the fish body. Scuticociliates were observed on the skin and gills right from day 0-1 post-infection, muscle tissue on day 2 post-infection, reached the brain, and spinal cord on day 3 post-infection, and systemic infection was prominent afterwards. Brain lesions were observed in most of the examined fish from days 3 and 4 post-infection and considered to be the cause of the sudden increase in mortality. Affected fish showed varying degrees of tissue damage including severe epidermal and dermal necrotic lesions, necrotic myositis, encephalitis and myelitis. Whereas, scuticociliates were frequently observed along the optic and/or olfactory nerve in the fish which were accompanied by severe brain lesions but by minimum lesions in the gills and skin, suggesting that in addition to skin and/or gills, neural routes including periorbital and nasal routes may play a role in scuticociliate invasion to the brain. Scuticociliates were also observed in the peripheral nerve fibers in the muscle tissue, cranial and spinal nerves, cranial cavity and in the vertebral canal, suggesting that nerve fibers and/or cerebrospinal fluid circulation may be involved in the spread of the scuticociliate throughout the body in addition to the blood circulation and connective tissue.

  4. EUROPEAN HARMONIZATION OF CONSOLIDATED FINANCIAL STATEMENTS REGULATIONS?

    National Research Council Canada - National Science Library

    Cirstea Andreea; Baltariu Carmen-Alexandra

    2012-01-01

    ... No. 1606/2002 adopted by the European Parliament and European Council on the 19th of July 2002, which regulates the application of IAS/IFRS regarding the financial reporting of listed European companies...

  5. Experiencing European integration: transnational lives and European identity

    NARCIS (Netherlands)

    Kuhn, T.

    2015-01-01

    European integration has generated a wide array of economic, political, and social opportunities beyond the nation state. Scandinavians spending their retirement at the Mediterranean coast; Germans employing a Polish caretaker; international exchange students staying in Prague; Italian baristas

  6. Intellectual property and biotechnology: the European debate.

    Science.gov (United States)

    Brody, Baruch

    2007-06-01

    The European patent system allows for the introduction of moral issues into decisions about the granting of patents. This feature has greatly impacted European debates about the patenting of biotechnology. This essay explores the European experience, in both the European Union and the European Patent Organization. It argues that there has been great confusion surrounding these issues primarily because the Europeans have not developed a general theory about when exclusion from patentability is the best social mechanism for dealing with morally offensive technologies.

  7. Old European Couples' Retirement Decisions

    DEFF Research Database (Denmark)

    Pozzoli, Dario; Ranzani, Marco

    This study investigates old European couples' retirement choices in order to bridge the gap between the European and the American literature. The typical European family approaching retirement is a dual-earner family: the dataset used in this paper reveals that 78 percent of working males...... correlated with education, age, and health status, together with partner's employment status, partner's education and partner's health status. We also perform a sensitivity analysis in order to check whether the results on the correlation of health status are robust to two alternative measures of health...

  8. The European Parliament and the European future of Albania

    Directory of Open Access Journals (Sweden)

    Skerdilajd Bajramaj

    2015-07-01

    Full Text Available On 06.24.2014 Albania was granted the status of candidate country. The foreign ministers of the 28 European Union member states decided unanimously to grant the candidate status for Albania. After receiving the status of “candidate country” for EU membership, the institutional relations with European international bodies are becoming stronger. One of these institutions, which during this time has increased its authority with Albania, is the European Parliament. In this paper will be analyzed the composition, competences and functioning of this particular institution, which is not only important for the future of the European Union and its Member States, but also for those who aim to join in. The study will be based on decisions made by this legislative body, as the only direct representative of the citizens of the European Union, and the impact they have on the performance and functioning of the Member States and the EU itself. In order to verify the validity of these claims, the analysis aims to assess the scope of the legislative function of the European Parliament post-Lisbon, examining its participation in the EU lawmaking both from the point of view of quantity and quality in over the past legislatures. Particular attention has been given to the examination of the changes that have taken place with the entry into force of the Lisbon Treaty, and the areas most affected. We will finally look at the work and contribution of the European Parliament, through the instruments at his disposal, on behalf of Albania’s progress towards full accession in the European Union.

  9. Public Accountability in the European Union: Is the European Parliament able to hold the European Council accountable?

    Directory of Open Access Journals (Sweden)

    Marianne van de Steeg

    2009-08-01

    Full Text Available The European Council occupies a central role in European politics. Yet it is not officially accountable to any public or parliamentary body for the decisions it makes on behalf of European citizens. National parliaments are only entitled to exert control over their own Heads of Government or State. The European Parliament, as a supranational institution, is the only parliamentary body that regularly discusses European politics with the European Council as a collective, namely via the European Council Presidency. As such, it alone has the possibility to hold the Council accountable for the agreements made at European Summits. However, the European Parliament has limited rights to impose pressure on the European Council Presidency. Nonetheless, despite the lack of a formal accountability arrangement, the European Council Presidency is more forthcoming than could have been expected.

  10. Post Crisis European Agenda

    Directory of Open Access Journals (Sweden)

    Pop, Napoleon

    2010-09-01

    Full Text Available The economic crisis is still evolving, at least from the point of view of some features making the entire process of its reversing very difficult - uncertainties, confusions and lack of investors` trust. More then that, every rim of the Atlantic Ocean has its own opinion as to the timing of the exit stimulus measures. There are prevailing risks taken into consideration when we talk about the next stage of the economic growth e.g. its sustainability on a log run or inflaming the inflation. It is for sure, that according to the core economic policies practiced either in USA or Europe, in Europe what count most is the danger of the inflation, if the right time of stopping the flood of the governmental funds to the real economy is not well chosen. The economic situation in Europe, as all over the world, in spite of some signs that the economy is on the right track ( South East Asia, USA or Germany, there are a lot of doubts as to the assurance that recession is over and there is no way for an immediate return. Notabilities in economics see that governmental intervention should be continued, just because the economic growth consigned by statistics is not convincing. Convictions, if they exist, are blended with a lot of obsessions which in my opinion are making up a serious agenda of the debates in Europe: budgetary deficit in excess, danger of the inflation, new regulation of the financial sector, credibility of the euro zone. Romania has to be involved in all these debates as it is passing through all the mentioned difficulties which need solutions within the frame of European standards, as a member of the EU.

  11. European Integration through Economic Convergence

    Directory of Open Access Journals (Sweden)

    Savoiu Gheorghe

    2016-05-01

    Full Text Available The thematic topicality of the main issues of European integration through convergence, in the context subsequent to financial-inspired global recession, is relevant through both the ever more extended international impact of the inertiality of a number of integration processes, and the specific developments of convergence in the Eurozone or the European Monetary Union (EMU. The regionally structured system of the European economy, approached aggregatively from the micro-economic to the macro-economic level, like the physical system or molecularly investigated system, from economic transactions, as a major milestone, to the complexity of the transactions tissue or flows, or activities, similar to biological systems, is permanently faced with various specific disturbing factors. The European Union (EU has historically evolved in an alternative manner, sometimes divergently or towards differentiation through separation, and sometimes convergently or coherently towards unity, fusion, integrity, evincing divergence or convergence trends, with the latter eventually becoming prevalent in the contemporary world.

  12. Renowned European Laboratory turns 50

    CERN Multimedia

    2004-01-01

    A European Laboratory that was the birthplace of the World Wide Web and home of Nobel prize-winning developments in the quest to understand the makeup of matter wished itself a happy 50th birtheday on Tuesday

  13. [French European military haemovigilance guidelines].

    Science.gov (United States)

    Sailliol, A; Clavier, B; Cap, A; Ausset, S

    2010-12-01

    European military transfusion services follow operational guidelines established by their respective national health systems and conform with European Union directives and NATO standards as applicable to member countries. Certain features are common to all of these standards, especially the pre-selection of volunteer, almost exclusively unpaid donors. NATO requirements are very close to European guidelines, with the exception that NATO permits the use of blood products collected in emergency conditions in theater when circumstances allow no better option. Blood product traceability exists for every country but is not always centralized or computerized. Serious adverse event reporting relies on national haemovigilance networks. Military considerations become important mainly in overseas operations, where the overall policy is to implement the relevant national, European or NATO guidelines with adjustments made for unique wartime circumstances and the risk/benefit ratio for the individual patient needing a transfusion. Copyright © 2010 Elsevier Masson SAS. All rights reserved.

  14. European Integration and environmental policy

    NARCIS (Netherlands)

    Ike, Paul; Bakker, Liesbeth

    1990-01-01

    There appears to be considerable enthusiasm about the expected macroeconomic consequences of European integration. If we regard integration, however, in the light of the environment and environmental policy, concern seems more appropriate. Predicted growth in production levels will damage the

  15. Papillomatosis in a European bison.

    Science.gov (United States)

    Literák, I; Tomita, Y; Ogawa, T; Shirasawa, H; Smíd, B; Novotny, L; Adamec, M

    2006-01-01

    Five European bison (Bison bonasus) from three European zoos were shipped to the Bukovské Vrchy Hills (Slovakia) in June 2004 and kept together in an acclimatization enclosure. The European bison were released into the wild in December 2004. At that time, papillomas were found at the medial canthus of the left eye of a 12-yr-old female bison. Cutaneous papillomatosis was confirmed histologically. Negative stain transmission electron microscopic examination revealed papillomavirus in the papillomas, and papillomavirus DNA also was detected using the polymerase chain reaction with FAP59 and FAP64 primers. The amplified 413 bp DNA sequence was identical to that of BAPV2 bovine papillomavirus. This paper is the first report of papillomatosis in European bison.

  16. European strategies for mental health.

    Science.gov (United States)

    Di Fiandra, Teresa

    2009-01-01

    The most recent developments of strategies and policies in the mental health field in Europe are related to the World Health Organization (WHO) Declaration and Action Plan on Mental Health signed by all the Ministers of Health of all Member States in the European Region (2005). The Action Plan proposes ways and means of developing comprehensive mental health policies, listing 12 areas in which challenges are indicated and detailed actions are required. Afterwards the Green Paper on Mental Health has been launched by the European Commission for the definition of an European strategy. The more precise European Pact for Mental Health and Well-being has been presented in 2008. Many other international bodies (OECD, Council of Europe, etc.) have actively worked to stress the mental health issue. All are clearly referring to the Italian model, started 30 years ago.

  17. European Hospitality Without a Home

    Directory of Open Access Journals (Sweden)

    Mireille Rosello

    2002-01-01

    Full Text Available How do European governments conceptualize what they call "hospitality" when they draft immigration laws and when they allow the concepts of asylum, of illegal immigrants, to change according to a constantly evolving political context? What consequences…

  18. Proton Football European Championship 2016

    CERN Multimedia

    2016-01-01

    Check out the European championship of proton football 2016 at CERN. Produced by: CERN Audiovisual Productions Service Director: Jacques Fichet Editor: Jacques Fichet Music : Burnt of Jingle Punks You can follow us on:

  19. Container Traffic In European Ports

    Directory of Open Access Journals (Sweden)

    Elen Twrdy

    2004-03-01

    Full Text Available Over the last fifteen years the European transport markethas witnessed a growth of container traffic which today reachesapproximately 50 million TEU per year. From 1997 to 2002,container traffic in the northern European ports increased from14 to 20.6 million TEU per year, in the ports of the westernMedite"anean from 6 to 10 million TEU per year, and in thenorthern Adriatic ports from 0. 69 to 0. 74 million TEU per year.The ports of the northern Adriatic are located in three states(Slovenia, Croatia and Italy with different statuses in relationto the common European market. In addition, different developmentlevels of these states are reflected in different levels ofinternational commercial exchange, the development of the existinginfrastructure and plans for the construction of new infrastructures.However, all three countries share a common goaltoincrease their competitiveness in comparison with the westemEuropean ports.

  20. European Space Agency Sentinel-2

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The Sentinel-2A satellite is operated by the European Space Agency (ESA). The Sentinel Multispectral Instrument (MSI) acquires 13 spectral bands ranging from Visible...

  1. NEW EUROPEAN PIPELINE PROJECT EASTRING

    OpenAIRE

    Karch, Lukáš; Varga, Augustín

    2018-01-01

    This paper focuses on the idea of a new European gas pipeline project called Eastring promoted by company Eustream. Eustream is the Slovak gas transmission system operator and has been one of the key players in European gas transmission from Russia to Europe in the last decades. Previous Russian-Ukrainian crisis resulted in reviewing gas flow directions from Russia to Europe in order to enhance the security of gas supplies to Europe. Russia plans to stop gas transmission to Europe via Ukraine...

  2. A European Open Science Cloud

    OpenAIRE

    EIROforum IT working group; CERN IT Department

    2015-01-01

    This document outlines the position of EIROforum on a European Open Science Cloud. It explores the essential characteristics of a European Open Science Cloud if it is to address the big data needs of the latest generation of Research Infrastructures. The high‐level architecture and key services as well as the role of standards is described. A governance and financial model together with the roles of the stakeholders, including commercial service providers and downstream business sectors, that...

  3. Innovation Policy in European Union

    Directory of Open Access Journals (Sweden)

    Marta-Christina Suciu

    2006-11-01

    Full Text Available The innovation policies aim to analyze priority factors shaping innovative performance and to reflect the increasing appreciation of the economic and social importance of innovation. This paper is commissioned to examine topics of current interest or concern to innovation policy-makers in Europe. Based on literature and the framework of the European Action Plan for Innovation, this paper investigates different levels and fields of European innovational systems and practices.

  4. Architecture of European plastic surgery.

    Science.gov (United States)

    Nicolai, J-P A; Banic, A; Molea, G; Mazzola, R; Poëll, J G

    2006-01-01

    The architecture of European Plastic Surgery was published in 1996 [Nicolai JPA, Scuderi N. Plastic surgical Europe in an organogram. Eur J Plast Surg 1996; 19: 253-256.] It is the objective of this paper to update information of that article. Continuing medical education (CME), science, training, examination, quality assurance and relations with the European Commission and Parliament all are aspects covered by the organisations to be discussed.

  5. The European Union's Africa Policy

    DEFF Research Database (Denmark)

    Olsen, Gorm Rye

    2013-01-01

    For a number of years, there has been an international debate on whether and to what extent small member states can influence the common external policies of the European Union. Recent research on the role of small EU states concludes that these states are neither per se political dwarfs nor powe...... place. Rather, it appears adequate to talk about convergence of policies between the Nordics and the EU and therefore, the Africa policies of both actors are basically the result of Europeanization....

  6. Principles of European Company Law

    DEFF Research Database (Denmark)

    Werlauff, Erik

    2010-01-01

    The article focuses on how much we have in common in Europe within company law and its development, and on the principles which are the determining factors for the activities of European companies. The article shows that what we have in common greatly outweighs what divides us......, and this is presented in the article as the provisional capstone of 400 years of joint European development of company law....

  7. European Languages: Instruments and Symbols

    Directory of Open Access Journals (Sweden)

    Martina Ožbot

    2008-07-01

    Further, the role of Latin as the single most important European language over the centuries and as a unifying feature of European culture is discussed. Parallels are drawn between Latin as the historical European lingua franca on the one hand and English as the modern language of international communication on the other: the importance of both languages started growing after substantial territorial expansion of their speakers and it was especially the political and economic power associated to these languages that played a significant role in their diffusion and long-term influence. Taking into consideration the instrumental as well as the symbolic function of languages, the question about the relationship between English and other European languages in today’s Europe is dealt with; it is suggested that the European languages are in principle not endangered as a result of the spread of English, with the exception of those instances in which English has been taking over the functions they have traditionally performed as national or community languages. It is emphasized that the future of Europe lies in the promotion of biand multilingualism, which have, in actual fact, been present on this continent throughout its history, and which in the cases of some European languages (e.g. Catalan, Basque, Irish, etc. have been successfully enhanced over the past decades.

  8. The globalization and Europeanization of mortgage markets

    NARCIS (Netherlands)

    Aalbers, M.B.

    2009-01-01

    Do globalization and Europeanization lead to the deterritorialization of European mortgage markets? Neither economic globalization nor EU policies have resulted in one European mortgage market. The various European mortgage markets are still quite different from one another in many respects. In most

  9. Environment in the European Union 1995

    DEFF Research Database (Denmark)

    The update to the 1992 report on the state of the environment in the European Union requested by the European Commission......The update to the 1992 report on the state of the environment in the European Union requested by the European Commission...

  10. Report from the 2012 European Gender Summit

    CERN Document Server

    European Gender Summit, 2012

    2012-01-01

    Report from the 2012 European Gender Summit to the European Parliament and the Council, the European Commission, the Council of Europe, EU Member and Associate States, Science Institutions. Developing Systematic Implementation Strategy to Advance EU Policy on Gender Equality in Science, as part of HORIZON 2020, European Research Area and Innovation Union.

  11. Introductory Guide to European Corporate Law

    DEFF Research Database (Denmark)

    Fomcenco, Alex

    Introductory Guide to European Corporate Law presents in an easily comprehensible and accessible way the main features and principles that govern European corporate law.......Introductory Guide to European Corporate Law presents in an easily comprehensible and accessible way the main features and principles that govern European corporate law....

  12. INTERNATIONAL AND EUROPEAN LAW PRINCIPLES

    Directory of Open Access Journals (Sweden)

    Dumitriţa FLOREA

    2016-06-01

    Full Text Available We live nowadays a posteriori establishment through institutional treaties and a well-built jurisprudence that set up some of the general treaties. The structure of the European Union as well as the particular nature of the community legal order led to the establishment of some general principles, such as the principle of the institutional balance, the principle of solidarity between member states, the principle of primacy, the principle of the immediate and direct effect of the European law. It is certain that the legal order of the community law has its basis in the legal order of the International public law from which it has continuously delineated. The European communities, as subjects of the International public law are obliged to comply with the general International law, its fundamental principles and its bases, leading to some obligations capable to produce effects in the community legal order. The Court Of Justice of the European Union established as principles applicable in the European law the principles of the International public law, especially when it was called to assert regarding the International relationships of the Community as well as those of the 28 member states, confirming in the matter of the applicability of the treaties, the pacta sunt servanda rule, the principle of the good faith, the legitimate faith or the rebus sic standibus exception, all of them belonging to the custom International law.

  13. The European Union's Eastward Enlargement

    Directory of Open Access Journals (Sweden)

    Hanns-D. Jacobsen

    1997-08-01

    Full Text Available In July 1997 the Commission of the European Union presented its Agenda 2000. This document not only suggested to start accession talks with some East and Central European countries (CEEC in early 1998 but it also provided proposals for a restructuring of EU expenditures. On this background this contribution discusses the state of readiness of the Central and East European countries to become EU members over the next few years and the Union's capacity to absorb up to ten CEEC over the next decade or so. This study concludes that the European Union has become an anchor of stability to the CEEC that guarantees both democracy and economic security--much more than could be expected only a couple of years ago. The Union's readiness to accept the membership of CEEC has, in a decisive way, contributed to their societal, political and economic stabilization. There is almost a contest among the CEEC on which one is going to meet the accession criteria first and best. The enlargement process, however, is connected to problems the resolution of which realistically postpone its successful conclusion at least into the year 2005. The main reasons are (1 the welfare gap between the EU area and the CEEC, (2 the need for more institutional and economic changes in the CEEC to make them ready for acces-sion, and, most importantly, (3 the necessity for the European Union to prepare herself for accession by a comprehensive financial reform and fundamental institutional changes.

  14. Response to the European Commission's consultation on the future of European company law

    NARCIS (Netherlands)

    Böckli, P.; Davies, P.; Ferran, E.; Ferrarini, G.; Garrido, J.; Hopt, K.; Pietrancosta, A.; Pistor, K.; Skog, R.; Soltysinski, S.; Winter, J.; Wymeersch, E.

    2012-01-01

    This paper contains the views of the European Company Law Experts (ECLE) on the future of European company law. The paper accompanies the responses of the European Company Law Experts to the European Commission’s Consultation on the future of European Company Law of spring 2012. In the first part of

  15. Interest Organisations and European Integration

    DEFF Research Database (Denmark)

    Pedersen, Ove K.

    . The paper focuses exclusively on the national policy processes that are involved with managing European Union (EU) issues. More specifically, this paper discusses two aspects of multi-level governance. First is the important role of private interests in the coordination of decision making at the national...... level preceding their government's representation of national interests in the European Council of Ministers and other EU organizations. Second is the effect of all this on national democratic systems.......This paper examines the influence of European integration on the relationship between state administration and private interests in the four Nordic countries - Sweden, Denmark, Norway and Finland. By private interests I mean interest organizations, private corporations and independent experts...

  16. European consumers and beef safety

    DEFF Research Database (Denmark)

    Van Wezemael, Lynn; Verbeke, Wim; Kügler, Jens Oliver

    2010-01-01

    consumption decision making process. Eight focus group discussions were performed with a total of 65 beef consumers in four European countries. Content analysis revealed that European consumers experienced difficulties in the assessment of the safety of beef and beef products and adopted diverging uncertainty......European beef consumption has been gradually declining during the past decades, while consumers' concerns about beef safety have increased. This paper explores consumer perceptions of and interest in beef safety and beef safety information, and their role in beef safety assessment and the beef...... reduction strategies. These include the use of colour, labels, brands and indications of origin as cues signalling beef safety. In general, consumer trust in beef safety was relatively high, despite distrust in particular actors....

  17. 4th European Turbulence Conference

    CERN Document Server

    1993-01-01

    The European Turbulence Conferences have been organized under the auspices of the European Mechanics Committee (Euromech) to provide a forum for discussion and exchange of recent and new results in the field of turbulence. The first conference was organized in Lyon in 1986 with 152 participants. The second and third conferences were held in Berlin (1988) and Stockholm (1990) with 165 and 172 participants respectively. The fourth was organized in Delft from 30 June to 3 July 1992 by the J.M. Burgers Centre. There were 214 participants from 22 countries. This steadily growing number of participants demonstrates both the success and need for this type of conference. The main topics of the Fourth European Turbulence Conference were: Dynamical Systems and Transition; Statistical Physics and Turbulence; Experiments and Novel Experimental Techniques; Particles and Bubbles in Turbulence; Simulation Methods; Coherent Structures; Turbulence Modelling and Compressibility Effects. In addition a special session was held o...

  18. Principles of European Contract Law

    DEFF Research Database (Denmark)

    Lando, Ole; Beale, Hugh

    of articles, with a detailed commentary explaining the purpose and operation of each article and its relation to the remainder. Each article also has extensive comparative notes surveying the national laws and other international provisions on the topic. "The Principles of European Contract Law Parts I &......This text provides a comprehensive guide to the principles of European contract law. They have been drawn up by an independent body of experts from each Member State of the EU, under a project supported by the European Commission and many other organizations. The principles are stated in the form......; II" cover the core rules of contract: formation, authority of agents, validity, interpretation, contents, performance, non-performance and remedies. The articles previously published in Part I (1995) are included in a revised and re-ordered form. Throughout Europe there is great interest...

  19. What Is Medieval European Literature?

    Directory of Open Access Journals (Sweden)

    Paolo Borsa

    2015-07-01

    Full Text Available The editors of Interfaces: A Journal of Medieval European Literatures explain the scope and purpose of the new journal by mapping out the significance and possible meanings of the three key terms of the subtitle: ‘literature,’ ‘medieval,’ ‘Europe.’ The specific theme of Issue 1 is introduced: ‘Histories of Medieval European Literatures: New Patterns of Representation and Explanation.’ With respect to this theme, theoretical problems concerning teleology and the present possibilities for literary historical narratives are raised. Finally the editors state the journal’s commitment to a scholarly forum which is non-profit and open-access. The bibliography refers to key critical reading which shapes the journal’s approach to medieval European literatures.

  20. The European Union's Normative Power

    DEFF Research Database (Denmark)

    Manners, Ian

    2011-01-01

    this period it became increasingly clear that the study of the EU in world politics needed to engage with both critical social theory and normative international theory. This chapter represents a return to critical social theory - a holistic research programme that works within a cosmopolitical understanding......What is the international identity of the European Union? The end of the cold war, the Treaty on European Union, and wars in Kuwait and the former Yugoslavia all raised methodological, theoretical and empirical questions over the study of the European Union's (EU) ‘international identity'. During...... of normative theory; that seeks to work ‘within categories of existing thought' regarding EU external actions, radicalise them and show ‘in varying degrees both their problems and their unrecognised possibilities' (Calhoun 1995: 23)....

  1. Constructing a European Governance Space

    DEFF Research Database (Denmark)

    Pontoppidan, Caroline Aggestam; Alijarde, Isabel Brusca; Chow, Danny

    . On the basis of that discussion, Eurostat launched another public consultation named; ‘Towards implementing European Public Sector Accounting Standards for EU member states-Public consultation on future EPSAS governance principles and structures’. This paper seeks to provide an in-depth understanding......Financial crisis has emphasized the need for harmonized public sector accounts in Europe. After a public consultation on the suitability of the IPSAS for EU Member States, the European Commission considers that the proper way is the development of European Union Standards adapted to IPSAS (renaming...... of the negotiations and interactions that took place prior to, during and after the issuance of the public consultation on the ‘Suitability of IPSAS’. The focus of this paper is therefore on a systematic analysis of the construction of regional-transnational governance of public sector accounting, using the EPSAS...

  2. European courts and old people.

    Science.gov (United States)

    Mulley, Graham P

    2013-09-01

    There are two major European Courts, the European Court of Justice (ECJ) and the European Court of Human Rights (ECHR). The ECJ deals with legal matters, mainly involving the interpretation of EU law and ensuring that the law is applied evenly across all 27 EU member states. The ECHR aims to make certain that civil and political rights of citizens in the 46 member states of the Council of Europe are observed. Most cases involving older citizens are about social policy (such as pension arrangements, equality, age discrimination and mandatory retirement). There have been few cases dealing with patients' rights, long-term care or housing. Referrals of selected cases involving old people should be considered if their rights are not being protected. In this Commentary, there is an account of how these Courts have evolved, together with guidance on whom to refer, to which Court, and when and how referrals should be made.

  3. The European Model Company Act

    DEFF Research Database (Denmark)

    Cleff, Evelyne Beatrix

    2011-01-01

    European Company Law regulation is currently undergoing a reform. These reforms raise a number of regulatory questions, such as what should be the aims of companies' legislation, and how these aims should best be met by regulation. Many of the reforms and discussions (both on EU and national level......) on the organisation of company laws reflect an interesting paradigm shift. Whereas, previously company law was primarily focused on preventing abuse, there is now a trend towards legislation that promote commerce and satisfy the needs of business. This means that the goal of economic efficiency is having...... an increasing influence on the framing of company legislation, such as the choice between mandatory or default rules. This article introduces the project 'European Company Law and the choice of Regulatory Method' which is carried out in collaboration with the 'European Model Company Act Group'. The project aims...

  4. 1. European Hydrogen Energy Conference

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-09-01

    This conference is the first of a series of EHA (European Hydrogen Association) conferences that will take place every two years in Europe with the collaboration of the national European Hydrogen Associations. EHEC 2003 takes place within the context of the debates on long term energy strategies organized by the international authorities and the governments of many countries. Under the patronage of the European Commission and the French government, the conference will aim at providing a comprehensive picture of the research work and demonstrations on hydrogen and fuel cells that the currently being carried out all over the globe. EHEC 2003 will provide an opportunity to define the role that hydrogen will have in tomorrow's energy landscape and, in particular, the benefits with regard to: 1)sustainable development of energy 2)control of climate change 3)development of renewable energy 4)increase demand for ground transport. (O.M.)

  5. Parameters of measuring of european political consciousness

    Directory of Open Access Journals (Sweden)

    M. M. Pikula

    2015-09-01

    Full Text Available In the article the author analyzes the parameters of European political consciousness, i.e. European research field of political consciousness in qualitative and quantitative terms, which may be based on different indicators. The issue of emergence and development of European political consciousness becomes topical because firstly, its formation as the subjective dimension of European integration policy is not a spontaneous process and, secondly, European integration is carried out not only from the top but from the bottom, requiring deliberate interference of the public with the process; the public possesses the formed European political consciousness. Since the latter is a specific mental construct, the author offers to apply the triad «criteria ­ parameters – indicators». The characteristic that makes it possible to evaluate certain processes or phenomena in the system of Europeanness / Europeanism and specifies the quality system of views and opinions, which are realized in European behavior, is considered to be the criterion of European political consciousness. The European political consciousness parameters are seen to include the relevant historical memory, trends of public opinion and awareness regarding the European Union and position of its members in the European integration process, including the assessment of the existence and development of the EU; knowledge and views on the main EU institutions, assessing the importance of the main institutions of the EU and trust in them; a positive vision for the future of the European Union etc. The author considers the performance and objective characteristics and dimensions, including positive correlation of national and European levels of identity (European identity and European behavior to be the indicatiors of European political awareness. On the basis of these indicators the control of the condition and trends of European political consciousness development will be carried out.

  6. Humanities in the European Union

    DEFF Research Database (Denmark)

    Johansson, Lasse Gøhler; Vikman, Jutta Maria; Liljenstrøm, Andreas Jan

    2017-01-01

    In the present paper, we analyze the publication strategies of researchers in the humanities, including their choices of language, publication type and co-authorship. Based on data from Denmark, we compare the publication profile of the humanities with the other major fields of science in 2012 an...... conjuncture of the European Union’s internationalization policies in the 1990s and 2000s and a change in the international scientific hierarchy during the 20th century has contributed to the universalization of English in the Danish (and European) scientific field....

  7. The European Synchrotron Radiation Facility

    DEFF Research Database (Denmark)

    Buras, B.; Materlik, G.

    1986-01-01

    In recent years, X-ray synchrotron radiation became a powerful tool for studies of condensed matter, and in view of that a proposal for the construction of a European Synchrotron Radiation Facility (ESRF) was elaborated in some detail by the European Synchrotron Radiation Project. The heart...... by a great flexibility and a small emittance (7×10−9 rad m) leading to a very high brilliance (1019 photons/(s mm2 mrad2) in a relative bandwidth of 0.1% in case of a 1 Å undulator). The overview, as seen from the users point of view, gives a brief account of the storage ring, emitted radiation...

  8. European Union a New Babylon?

    Science.gov (United States)

    Mesch, F.

    2010-07-01

    The growing European Union faces growing problems in personal communication. These problems cannot be overcome only by more language courses in school. As important is a better mutual knowledge of the culture of other countries, a knowledge that can be gained only by a personal, professional stay in foreign countries. On university level, such stays are best organized by networks connecting European universities. In the broad field of measurement, this IMEKO symposium might offer a unique forum to thoroughly discuss structure and realization of such a network with all interested colleagues.

  9. Passionate patriotism or European enslavement

    Directory of Open Access Journals (Sweden)

    Manić Mihajlo P.

    2015-01-01

    Full Text Available The study A passionate patriotism or European enslavement, from the eminent professor Petar Anđelković, represents another attempt by this author to analyze the Serbian society in the context of its development. This book is a logical continuation of his essay published in 2013, Serbian Society-without past, what is our present?, and represents a symbiosis of texts that the author has already published and in which he addresses the very relevant questions of Serbia's relations with the new world order and with the inevitable role of the European Union, as an existential challenge to the very existence of the Serbian society.

  10. The European Hematology Association Roadmap for European Hematology Research

    DEFF Research Database (Denmark)

    Engert, Andreas; Balduini, Carlo; Brand, Anneke

    2016-01-01

    research. Initiated by the EHA, around 300 experts contributed to the consensus document, which will help European policy makers, research funders, research organizations, researchers, and patient groups make better informed decisions on hematology research. It also aims to raise public awareness...

  11. Development of technologies to prevent fish diseases by vaccination. 2. Effect of preparation method and administer dose on a vaccine against dewardsiellosis of Japanese flounder; Wakuchin ni yoru gyobyo yobo gijutsu no kaihatsu. 2. Hirame no edowarajesho ni taisuru wakuchin no choseiho oyobi toyoryo no kento

    Energy Technology Data Exchange (ETDEWEB)

    Furuta, T.; Kikuchi, K.; Iwata, N.

    1999-09-01

    Immunization experiments were conducted to decide optimum administer condition of injection vaccine against edwardsiellosis of Japanese flounder. Effect of the vaccine was estimated with challenge test, exposing the flounder to live Edwardsiella tarda after about 1 month of vaccination. 1) At the same dose formalin-killed vaccine was more effective than heat-killed. Growth of the fish, injected heat-killed vaccine at a dose of 100 mg per 100 g of fish weighing was inferior to control, however, E. tarda were not re-isolated from all the survived fish in the challenge test. Incubation and starvation time of bacterial cultivation, 24 to 96 h and 0 to 48 h, respectively, did not change the effect of formalin-killed vaccine. Among the experimental groups, the largest quantity of bacterin was obtained after 48 h of incubation and 0 h of starvation. 2) The optimum administer dose of formalin-killed vaccine was around 10 mg per 100 g of fish weighing, however. E. tarda were re-isolated from survived fish after challenge test. The duration of protective immunity was about 90 days. (author)

  12. The European Hematology Association Roadmap for European Hematology Research : A consensus document

    NARCIS (Netherlands)

    Engert, Andreas; Balduini, Carlo; Brand, Anneke; Coiffier, Bertrand; Cordonnier, Catherine; Döhner, Hartmut; de Wit, Thom Duyvené; Eichinger, Sabine; Fibbe, Willem; Green, Tony; de Haas, Fleur; Iolascon, Achille; Jaffredo, Thierry; Rodeghiero, Francesco; Salles, Gilles; Schuringa, Jan Jacob

    The European Hematology Association (EHA) Roadmap for European Hematology Research highlights major achievements in diagnosis and treatment of blood disorders and identifies the greatest unmet clinical and scientific needs in those areas to enable better funded, more focused European hematology

  13. The European Hematology Association Roadmap for European Hematology Research : a consensus document

    NARCIS (Netherlands)

    Engert, Andreas; Balduini, Carlo; Brand, Anneke; Coiffier, Bertrand; Cordonnier, Catherine; Döhner, Hartmut; de Wit, Thom Duyvené; Eichinger, Sabine; Fibbe, Willem; Green, Tony; de Haas, Fleur; Iolascon, Achille; Jaffredo, Thierry; Rodeghiero, Francesco; Salles, Gilles; Schuringa, Jan Jacob

    The European Hematology Association (EHA) Roadmap for European Hematology Research highlights major achievements in diagnosis and treatment of blood disorders and identifies the greatest unmet clinical and scientific needs in those areas to enable better funded, more focused European hematology

  14. Scientific education and European citizenship. Suggestions and results from the European project SEDEC

    Directory of Open Access Journals (Sweden)

    Roberto Ceriani

    2007-09-01

    Full Text Available Two concepts seemingly distant from each other, scientific education and European citizenship, have been the basis for "SEDEC - Science Education for the Development of European Citizenship", a European project funded by the European Commission in the framework of the Socrates/Comenius programme, aiming at producing training material addressed to European teachers. Started in autumn 2005, the project will end in 2008 with an in-service training course for European teachers and educators.

  15. Scientific education and European citizenship. Suggestions and results from the European project SEDEC (Italian original version

    Directory of Open Access Journals (Sweden)

    Roberto Ceriani

    2007-09-01

    Full Text Available Two concepts seemingly distant from each other, scientific education and European citizenship, have been the basis for "SEDEC - Science Education for the Development of European Citizenship", a European project funded by the European Commission in the framework of the Socrates/Comenius programme, aiming at producing training material addressed to European teachers. Started in autumn 2005, the project will end in 2008 with an in-service training course for European teachers and educators.

  16. Airline competition at European airports

    NARCIS (Netherlands)

    Frenken, K.; van Terwisga, S.; Verburg, T.; Burghouwt, G.

    2004-01-01

    Hub-and-spoke networks of airlines create entry barriers at large hub airports. As a result, deregulation does not necessary lead to more competition. In this paper, airline competition at European airports in the 1990s is analysed. Results show important differences between airports, which are

  17. 5th European Rheology Conference

    CERN Document Server

    1998-01-01

    Global sustainable development of the world economy requires better understanding and utilization of natural recourses. In this endeavor rheology has an indispensable role. The Rheology Conferences are therefore always an important event for science and technology. The Fifth European Rheology Conference, held from September 6 to 11 in the Portoro-z, Slovenia, will be the first AlI-European rheology meeting after the formal constitution of the European Society ofRheology. As such it will be a special historical event. At this meeting the European Society of Rheology will introduce the Weissenberg Medal, to be bestowed every four years to an individual for hislhers contribution to the field of Rheology. The recipient ofthe first award will be professor G. Marrucci ofthe Universita degli Studi di Napoli, Italy. Two mini Symposia will be part of the Conference. The first, on Industrial Rheology, will commemorate the late professor G. Astarita. The second will honor the eightieth birthday of professor N.W. Tschoeg...

  18. European Bat Lyssaviruses, the Netherlands

    NARCIS (Netherlands)

    Poel, van der W.H.M.; Heide, van der R.; Verstraten, E.R.A.M.; Kramps, J.A.

    2005-01-01

    To study European bat lyssavirus (EBLV) in bat reservoirs in the Netherlands, native bats have been tested for rabies since 1984. For all collected bats, data including species, age, sex, and date and location found were recorded. A total of 1,219 serotine bats, Eptesicus serotinus, were tested, and

  19. Integration of European Bond Markets

    DEFF Research Database (Denmark)

    Christiansen, Charlotte

    2014-01-01

    I investigate the time variation in the integration of EU government bond markets. The integration is measured by the explanatory power of European factor portfolios for the individual bond markets for each year. The integration of the government bond markets is stronger for EMU than non...

  20. A European Social Simulation Association

    NARCIS (Netherlands)

    Moss, S

    This is a proposal to form a European Social Simulation Association (ESSA). ESSA is intended to coordinate with similar organisations in North America, Latin America and Australasia and other regions to organise an international federation to support the development of social simulation research,

  1. The Big European Bubble Chamber

    CERN Multimedia

    1977-01-01

    The 3.70 metre Big European Bubble Chamber (BEBC), dismantled on 9 August 1984. During operation it was one of the biggest detectors in the world, producing direct visual recordings of particle tracks. 6.3 million photos of interactions were taken with the chamber in the course of its existence.

  2. The common European flexicurity principles

    DEFF Research Database (Denmark)

    Mailand, Mikkel

    2010-01-01

    This article analyses the decision-making process underlying the adoption of common EU flexicurity principles. Supporters of the initiative succeeded in convincing the sceptics one by one; the change of government in France and the last-minute support of the European social partner organizations...

  3. Biotechnology and the European public

    NARCIS (Netherlands)

    Gaskell, George; Allum, Nick; Bauer, Martin; Durant, John; Allansdottir, Agnes; Bonfadelli, Heinz; Boy, Daniel; de Cheveigne, Suzanne; Fjaestad, Bjorn; Gutteling, Jan M.; Hampel, Juergen; Jelsoe, Erling; Correia Jesuino, Jorge; Kohring, Matthias; Kronberger, Nicole; Midden, Cees; Hviid Nielsen, Torben; Przestalski, Andrzej; Rusanen, Timo; Sakellaris, George; Torgersen, Helge; Twardowski, Tomasz; Wagner, Wolfgang

    2000-01-01

    The latest European sample survey of public perceptions of biotechnology reveals widespread opposition to genetically modified (GM) food in much of Europe, but public attitudes to medical and environmental applications remain positive. Data from the fourth Eurobarometer survey carried out in

  4. Essays on European bond markets

    NARCIS (Netherlands)

    Cheung, Y.C.

    2005-01-01

    This dissertation focused on a number of issues that are of importance in the current European bond market. In the past years, the fiscal policy of the Eurozone members, advances in the technology of trading platforms and the introduction of a single currency have reshaped the fixed income markets

  5. Virtual Models of European Universities

    DEFF Research Database (Denmark)

    Pedersen, Sanya Gertsen

    2003-01-01

    The study provides a detailed report on the current and possible future use of ICT by European universities for educational and organisational purposes. The report presents: • A general description of the current situation regarding the use of ICT in EU universities in both the educational...

  6. European consumers and nutrition labelling

    DEFF Research Database (Denmark)

    Wills, Josephine M.; Grunert, Klaus G.; Celemín, Laura Fernández

    2009-01-01

    Nutrition labelling of food in Europe is not compulsory, unless a nutrition or health claim is made for the product. The European Commission is proposing mandatory nutrition labelling, even front of pack labelling with nutrition information. Yet, how widespread is nutrition labelling in the EU...

  7. Architecture of European Plastic Surgery

    NARCIS (Netherlands)

    Nicolai, J. -P. A.; Banic, A.; Molea, G.; Mazzola, R.; Poell, J. G.

    2006-01-01

    The architecture of European Plastic Surgery was published in 1996 [Nicolai JPA, Scuderi N. Plastic surgical Europe in an organogram. Eur J Plast Surg 1996; 19: 253-6.] It is the objective of this paper to update information of that article. Continuing medical education (CME), science, training,

  8. Scholarships for European vet students.

    Science.gov (United States)

    Egan, Jordon; Takacs, Daniella

    2017-02-25

    Every year, students from European vet schools can apply for $5000 scholarships, which aim to 'enhance the academic experience of students'. Among last year's recipients were Jordon Egan, from the Royal Veterinary College, and Daniella Takács, from the University of Veterinary Medicine in Budapest. British Veterinary Association.

  9. Impacts of European livestock production

    NARCIS (Netherlands)

    Leip, Adrian; Billen, Gilles; Garnier, Josette; Grizzetti, Bruna; Lassaletta, Luis; Reis, Stefan; Simpson, David; Sutton, M.A.; Vries, De Wim; Weiss, Franz; Westhoek, Henk

    2015-01-01

    Livestock production systems currently occupy around 28% of the land surface of the European Union (equivalent to 65% of the agricultural land). In conjunction with other human activities, livestock production systems affect water, air and soil quality, global climate and biodiversity, altering

  10. Towards a European contract law

    NARCIS (Netherlands)

    Hondius, E.H.

    2000-01-01

    I. Introduction. II. Subject-matter of this paper. III. Constitutionality. IV. Codification. V. Is it Feasible?VI. Howto proceed. VII. New problems: finding the Law. VIII. The Netherlands, Belgium and Germany. IX. Common Law and Civil Law. X. East and west. XI. European Community Law. XII.

  11. Governance and European Civil Society

    DEFF Research Database (Denmark)

    Kutay, Acar

    This book provides a critical analysis of the European Union’s approach to ‘governance’, focusing on the way in which civil society is incorporated within the EU decision-making process and arguing that it is not conducive to the democratisation of EU governance.\

  12. Mining in the European Arctic

    NARCIS (Netherlands)

    van Dam, Kim; Scheepstra, Annette; Gille, Johan; Stępień, Adam; Koivurova, Timo

    The European Arctic is currently experiencing an upsurge in mining activities, but future developments will be highly sensitive to mineral price fluctuations. The EU is a major consumer and importer of Arctic raw materials. As the EU is concerned about the security of supply, it encourages domestic

  13. Obesogenic Diets in European Children

    DEFF Research Database (Denmark)

    Intemann, Timm; Hebestreit, Antje; Reisch, Lucia A.

    2017-01-01

    Introduction: Poor nutrition is a major contributor to the overall burden of disease. Worldwide, nutrition-related diseases have become a major health concern, reportedly causing a loss of over 56 million years of healthy life for European citizens in the year 2000. Childhood obesity is one of th...

  14. Drugs in society: European perspectives

    NARCIS (Netherlands)

    Fountain, J.; Korf, D.J.

    2007-01-01

    This unique overview of the variation in the ways recreational and other drugs are used across Europe includes critical reflections on current drug policy. Contributions from a wide range of professionals and academics in different countries offer a truly international perspective on the European

  15. European Cyber Security Perspectives 2015

    NARCIS (Netherlands)

    Baloo, J.; Geveke, H.G.; Paulissen, W.; Vries, H. de

    2015-01-01

    Following the success of last year’s publication, we are proud to present the second edition of our European Cyber Security Perspectives report. Through this collection of articles, we aim to share our different perspectives and insights, the latest developments and achievements in the field of

  16. The European NEO Coordination Centre

    Science.gov (United States)

    Perozzi, E.; Borgia, B.; Micheli, M.

    An operational approach to NEO (Near-Earth Object) hazard monitoring has been developed at European level within the framework of the Space Situational Awareness Program (SSA) of the European Space Agency (ESA). Through federating European assets and profiting of the expertise developed in European Universities and Research Centers, it has been possible to start the deployment of the so-called SSA NEO Segment. This initiative aims to provide a significant contribution to the worldwide effort to the discovery, follow-up and characterization of the near-Earth object population. A major achievement has been the inauguration in May 2013 of the ESA NEO Coordination Centre located at ESRIN (Frascati, Italy). The goal of the NEOCC Precursor Service operations is twofold: to make available updated information on the NEO population and the associated hazard and to contribute to optimize the NEO observational efforts. This is done by maintaining and improving a Web Portal publicly available at http://neo.ssa.esa.int and by performing follow-up observations through a network of collaborating telescopes and facilities. An overview of the SSA-NEO System and a summary of the first two years of NEOCC operations is presented.

  17. The European radon mapping project

    Energy Technology Data Exchange (ETDEWEB)

    Bossew, P., E-mail: pbossew@bfs.de [German Federal Office for Radiation Protection, Berlin (Germany); Tollefsen, T.; Gruber, V.; De Cort, M., E-mail: tore.tollefsen@jrc.ec.europa.eu, E-mail: valeria.gruber@gmail.com, E-mail: marc.de-cort@jrc.ec.europa.eu [Institute for Transuranium Elements, Ispra, VA (Italy). DG Joint Research Centre. European Commission

    2013-07-01

    There is almost unanimous agreement that indoor radon (Rn) represents a hazard to human health. Large-scale epidemiological studies gave evidence that Rn is the second-most important cause o flung cancer after smoking and that also relatively low Rn concentrations can be detrimental. This has increasingly led to attempts to limit Rn exposure through regulation, mainly building codes. The proposed Euratom Basic Safety Standards (BSS) require Member States to establish Rn action plans aimed at reducing Rn risk, and to set reference values for Imitating indoor Rn concentration. In 2006 the JRC started a project on mapping Rn at the European level, in addition and complementary lo (but not as a substitute for) national efforts. These maps are part of the European Atlas of Natural Radiation project. which is planned eventually 10 comprise geographical assessments of ali sources of exposure to natural radiation. Started first, a map of indoor Rn is now in an advanced phase, but still incomplete as national Rn surveys are ongoing in a number of European countries. A European map of geogenic Rn, conceptually and technically more complicated, was started in 2008. The main difficulty encountered is heterogeneity of survey designs, measurement and evaluation methods and database semantics and structures. An important part or the work on the Atlas is therefore to harmonize data and methods. We present the current state of the Rn maps and discuss some of the methodological challenges. (author)

  18. The European Approach to Privacy

    NARCIS (Netherlands)

    van Hoboken, J.

    2014-01-01

    This paper critically assesses the character of European (Union’s) privacy law and policy in the field of online media and electronic communications. Contrary to current understanding, this field of law is more fragmented and ill-developed than is often assumed, in particular by those discussing

  19. The European Location Framework - from National to European

    Science.gov (United States)

    Pauknerova, E.; Sidlichovsky, P.; Urbanas, S.; Med, M.

    2016-06-01

    The European Location Framework (ELF) means a technical infrastructure which will deliver authoritative, interoperable geospatial reference data from all over Europe for analysing and understanding information connected to places and features. The ELF has been developed and set up through the ELF Project, which has been realized by a consortium of partners (public, private and academic organisations) since March 2013. Their number increased from thirty to forty in the year 2016, together with a project extension from 36 to 44 months. The project is co-funded by the European Commission's Competitiveness and Innovation Framework Programme (CIP) and will end in October 2016. In broad terms, the ELF Project will deliver a unique gateway to the authoritative reference geospatial information for Europe (harmonised pan-European maps, geographic and land information) sourced from the National Mapping and Cadastral Authorities (NMCAs) around Europe and including transparent licensing. This will be provided as an online ELF web service that will deliver an up-to-date topographic base map and also as view & download services for access to the ELF datasets. To develop and build up the ELF, NMCAs are accompanied and collaborate with several research & academia institutes, a standardisation body, system integrators, software developers and application providers. The harmonisation is in progress developing and triggering a number of geo-tools like edge-matching, generalisation, transformation and others. ELF will provide also some centralised tools like Geo Locator for searching location based on geographical names, addresses and administrative units, and GeoProduct Finder for discovering the available web-services and licensing them. ELF combines national reference geo-information through the ELF platform. ELF web services will be offered to users and application developers through open source (OSKARI) and proprietary (ArcGIS Online) cloud platforms. Recently, 29 NMCAs plus the

  20. European Marine Information System. Eumaris; European Marine Information System. Eumaris

    Energy Technology Data Exchange (ETDEWEB)

    Caiaffa, E. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dipt. Ambiente

    1999-07-01

    The present paper summarises the activities developed by ETC/MCE, under European Environment Agency (EEA) contract, on the Geographic Information System. The aim of present paper is to introduce some general concepts about the Geographic Information Systems and to investigate the potential of G.I.S. as tool for the assessment of the European seas. Care has also been taken to explain the main technical and educational reasons that led to EUMARIS GIS birth, its evolution in the last year, till to its presentation at the Inter-Regional Forum of the European Conventions held in Venice. GIS is a technological tool phenomenon involving various aspects and different issues; many examples of thematic maps involving that different subjects are shown in the paper. [Italian] Il presente articolo riassume le attivita' svolte, nell'ambito del Geographic Information System, dall'ETC/MCE sotto contratto con l'Agenzia Europea per l'Ambiente (EEA) che ha sede a Copenhagen. Scopo del presente articolo e' quello di introdurre alcuni concetti generali sui Geographic Information System (GIS) e di dimostrare la possibilita' di utilizzare il GIS stesso come strumento per la valutazione dello stato dei mari dell'Europa. Si e' cercato di spiegare le principali ragioni tecniche che hanno portato alla nascita del progeto del GIS EUMARIS, alla sua realizzazione fino alla sua presentazione all'Inter-Regional Forum of European Conventions tenutosi a Venezia. Si e' cercato anche di mostrare come un GIS per sua natura e' uno strumento che coinvolge vari aspetti tecnologici e differenti tipi di dati; nell'articolo vengono mostrati diversi esempi di mappe tematiche che contengono tali differenti argomenti.

  1. THE EUROPEAN LOCATION FRAMEWORK – FROM NATIONAL TO EUROPEAN

    OpenAIRE

    Pauknerova, E.; P. Sidlichovsky; Urbanas, S.; Med, M.

    2016-01-01

    The European Location Framework (ELF) means a technical infrastructure which will deliver authoritative, interoperable geospatial reference data from all over Europe for analysing and understanding information connected to places and features. The ELF has been developed and set up through the ELF Project, which has been realized by a consortium of partners (public, private and academic organisations) since March 2013. Their number increased from thirty to forty in the year 2016, toge...

  2. European climate change experiments on precipitation change

    DEFF Research Database (Denmark)

    Beier, Claus

    Presentation of European activities and networks related to experiments and databases within precipitation change......Presentation of European activities and networks related to experiments and databases within precipitation change...

  3. TOWARDS A POLITICAL SOCIOLOGY OF EUROPEAN IDENTITY

    Directory of Open Access Journals (Sweden)

    LUCIAN-ŞTEFAN DUMITRESCU

    2015-05-01

    Full Text Available David McCrone pointed that the odds for the emergence of a European identity were quite feeble ten years ago. He claimed that surfacing a European identity needed at least three conditions, namely a pivotal nation to organize the European culture around its particular culture, a well-defined otherness, and a clear-cut political model. I argue that among the three conditions deemed necessary for the emergence of a European identity by McCrone, only the last one, that is, the political model of the European Union, still needs to be clarified. The other two have already been fulfilled. Nowadays, Germany seems to be the pivotal nation around which the European Union revolves politically and economically, whilst clear-cut Internal and External Significant Others of the European Union have already been forged. The article is a a foray into the political mechanisms of creating the Internal and External Significant Others of the European Union

  4. North European Symposium for Archaeological Textiles X

    DEFF Research Database (Denmark)

    Proceedings of the Tenth North European Symposium for Archaeological Textiles, held in Copenhagen, 14-17 May 2008......Proceedings of the Tenth North European Symposium for Archaeological Textiles, held in Copenhagen, 14-17 May 2008...

  5. The Citizen, the Culture and European Union

    National Research Council Canada - National Science Library

    Marine Imberechts

    2009-01-01

    From the beginning of its work (1988), the European Centre of Culture tried to attract theattention of the European institutions on the urgent need to bring in the greatest number of citizens asense of belonging to a new space...

  6. European labs fight back against cuts

    CERN Multimedia

    König, R

    1997-01-01

    Germany's 1997 budget contains cuts amounting to 3.7% in funding of domestic research programs and in contributions to international labs. Contributions will be cut to the European Space Agency, the European Synchrotron facility and CERN.

  7. Symbols and Myths in European Integration

    DEFF Research Database (Denmark)

    Lynggaard, Kennet; Manners, Ian James; Søby, Christine

    2013-01-01

    The study of symbols and myths in European integration is crucial to our understanding of both how the European Union (EU) becomes constituted as a political reality and how the integration process itself occurs. By drawing on the study of symbols and myths from political science, humanities...... and cultural studies to the analysis of European integration, this paper will set out a project to provide a better understanding of how symbolic and substantial processes interact in European society....

  8. Sensory quality criteria for five fish species

    DEFF Research Database (Denmark)

    Warm, Karin; Nielsen, Jette; Hyldig, Grethe

    2000-01-01

    Sensory profiling has been used to develop one sensory vocabulary for five fish species: cod (Gadus morhua), saithe (Pollachius virens), rainbow trout (Salmo gardineri), herring (Clupea harengus) and flounder (Platichthys flessus). A nine- member trained panel assessed 18 samples with variation...... for odor and taste during the qualitative part. The descriptive words should fulfil these criteria: be relevant to the product, discriminate clearly between samples, be nonredundant and have cognitive clarity to the assessors. Criteria fulfilment was reached by evaluating samples spanning a representative...

  9. Arsenic concentrations correlate with salinity for fish taken from the North Sea and Baltic waters

    DEFF Research Database (Denmark)

    Larsen, Erik Huusfeldt; Francesconi, K. A.

    2003-01-01

    Total arsenic concentrations were determined in three teleost species (herring Clupea harengus; cod Gadus morhua, and flounder Platichthys flesus) taken. from four locations in the Baltic and North Sea with salinities ranging from 8 to 32 psu. Individual arsenic concentrations ranged from 0.......04 to 10.9 mg/kg wet mass, and there was a positive linear relationship between arsenic concentration and salinity for all three species (r(2) 0.44 to 0.72, all P ... here are the first showing a relationship between the total arsenic concentration in fish and salinity....

  10. Globalized Trade, Logistics and Intermodality: European Perspectives

    NARCIS (Netherlands)

    Henstra, D.; Ruijgrok, C.J.; Tavasszy, L.A.

    2007-01-01

    This chapter describes megatrends that are shaping international trade, logistics organization and (multi)modal transport in Europe. It focuses on impacts on the European context, both from the peculiarities arising from the European unification process and the European transport policies but

  11. Volatility Spillover Effects in European Equity Markets

    NARCIS (Netherlands)

    Baele, L.

    2003-01-01

    This paper quantifies the magnitude and time-varying nature of volatility spillovers from the aggregate European (EU) and US market to 13 local European equity markets.I develop a shock spillover model that decomposes local unexpected returns into a country speciffic shock, a regional European

  12. European Initiatives in Postgraduate Education in Gerontology

    Science.gov (United States)

    van Rijsselt, Rene J. T.; Parkatti, Terttu; Troisi, Joseph

    2007-01-01

    This paper describes three innovative European initiatives in postgraduate education in gerontology. The first is the European Masters Program in Gerontology (EuMaG), developed as an interdisciplinary joint program, supported and delivered by 22 European universities. Second, the Nordplus initiative to increase mobility of students and staff in…

  13. The politics of a European civil code

    NARCIS (Netherlands)

    Hesselink, M.W.

    2004-01-01

    Last year the European Commission published its Action Plan on European contract law. That plan forms an important step towards a European Civil Code. In its Plan, the Commission tries to depoliticise the codification process by asking a group of academic experts to prepare what it calls a 'common

  14. Conditions for spatial segregation: some European perspectives.

    NARCIS (Netherlands)

    Musterd, S.; de Winter, M.

    1998-01-01

    Evaluates some theses on the theme of spatial segregation in Europe. Spatial segregation as an important issue on the political agendas of European nations; Two views of segregation in Europe; Strategies of European nations to deal with segregation; Segregation in European cities

  15. Flexibilization of labour in the European Union

    NARCIS (Netherlands)

    Holman, O.; van der Pijl, K.

    2015-01-01

    Labour market flexibilization has been at the heart of the ‘extended relaunch’ of European integration (the completion of the Single Market and the introduction of the euro in 2002) from the start in the 1980s. Yet the parallel Europeanization of labour market policies, via the European Employment

  16. European Conference „Meetbike“. Information

    Directory of Open Access Journals (Sweden)

    Gerd-Axel AHRENS

    2008-01-01

    Full Text Available The information is devoted European Conference „Meetbike“ 3-4 April2008 in Dresden, Germany. Stronger networking of European bicycle-friendly cities and better cooperation of bike use and public transport has been considered. Results of the European Conference „MeetBike“ are presented.

  17. The Political History of European Integration

    DEFF Research Database (Denmark)

    Schulz-Forberg, Hagen; Stråth, Bo

    This book adopts a historical perspective to explore the tensions between the idea of a European democracy through a European market, and the observations that there are signs of increasing social disintegration, political extremism and populism in the wake of economic integration. It was shortli....... It was shortlisted for the European Book Prize 2011...

  18. European Union Financial Crisis: A Marxist Analysis

    NARCIS (Netherlands)

    Petrus Kanisius Farneubun, P.

    European financial crisis poses a serious challenge to the fundamental structure of the European Union, political and financial institutions, as well as the values that bind European together. Different factors have been suggested as the causes of the crisis notably the failure of national

  19. MAPPING EUROPEAN CAPITAL MARKETS TENDENCIES

    Directory of Open Access Journals (Sweden)

    Andreea Avadanei

    2011-01-01

    Full Text Available The scope of this paper is to analyze the main tendencies influencing European capitalmarkets development. In order to point out their implications, we structured our study onfour chapters. The first one illustrates the consolidation of stock exchanges at the Europeanlevel; the seconds presents some considerations about the deregulation/re-regulation of EUcapital markets; the third section highlights the globalization implications on their evolutionand the fourth one indicates the effects of financial innovation. The progressive integrationof European capital markets over the past decade contributed to the lowering of capital costas a result of increased risk diversification opportunities and reduced transaction costs dueto the specialization of financial system provisions. Moreover, the expansion of thesemarkets improved firms financing decisions, leading to an increased share of non-bankfunding sources.

  20. Benchmarking & European Sustainable Transport Policies

    DEFF Research Database (Denmark)

    Gudmundsson, H.

    2003-01-01

    to support Sustainable European Transport Policies. The key message is that transport benchmarking has not yet been developed to cope with the challenges of this task. Rather than backing down completely, the paper suggests some critical conditions for applying and adopting benchmarking for this purpose. One...... way forward is to ensure a higher level of environmental integration in transport policy benchmarking. To this effect the paper will discuss the possible role of the socalled Transport and Environment Reporting Mechanism developed by the European Environment Agency. The paper provides an independent......, Benchmarking is one of the management tools that have recently been introduced in the transport sector. It is rapidly being applied to a wide range of transport operations, services and policies. This paper is a contribution to the discussion of the role of benchmarking in the future efforts...

  1. Sentinel European Node Trial (SENT)

    DEFF Research Database (Denmark)

    Schilling, C.; Stoeckli, S. J.; Haerle, S. K.

    2015-01-01

    Purpose: Optimum management of the N0 neck is unresolved in oral cancer. Sentinel node biopsy (SNB) can reliably detect microscopic lymph node metastasis. The object of this study was to establish whether the technique was both reliable in staging the N0 neck and a safe oncological procedure...... in patients with early-stage oral squamous cell carcinoma. Methods: An European Organisation for Research and Treatment of Cancer-approved prospective, observational study commenced in 2005. Fourteen European centres recruited 415 patients with radiologically staged T1-T2N0 squamous cell carcinoma. SNB...... was undertaken with an average of 3.2 nodes removed per patient. Patients were excluded if the sentinel node (SN) could not be identified. A positive SN led to a neck dissection within 3 weeks. Analysis was performed at 3-year follow-up. Results: An SN was found in 99.5% of cases. Positive SNs were found in 23...

  2. Osteoporosis in the European Union

    DEFF Research Database (Denmark)

    Svedbom, A; Hernlund, E; Ivergård, M

    2013-01-01

    UNLABELLED: This report describes epidemiology, burden, and treatment of osteoporosis in each of the 27 countries of the European Union (EU27). INTRODUCTION: In 2010, 22 million women and 5.5 million men were estimated to have osteoporosis in the EU; and 3.5 million new fragility fractures were...... years lost during 2010. The costs are expected to increase by 25 % in 2025. The majority of individuals who have sustained an osteoporosis-related fracture or who are at high risk of fracture are untreated and the number of patients on treatment is declining. The aim of this report was to characterize...... the burden of osteoporosis in each of the EU27 countries in 2010 and beyond. METHODS: The data on fracture incidence and costs of fractures in the EU27 were taken from a concurrent publication in this journal (Osteoporosis in the European Union: Medical Management, Epidemiology and Economic Burden...

  3. European global navigation satellite launches

    Science.gov (United States)

    Zielinski, Sarah

    The European Space Agency launched its first Galileo satellite on 28 December 2005.When fully deployed, the Galileo system will provide a European global navigation alternative to the U.S. global positioning system (GPS) and the Russian global navigation satellite system (Glonass).The Galileo system will consist of 30 satellites (27 operational plus three active spare satellites) that are scheduled to be launched and fully operational by the end of 2008.The system will provide real-time positioning within one meter of accuracy and be fully inter-operable with the U.S. and Russian systems. However, unlike GPS and Glonass, Galileo will be under civilian rather than military control.

  4. European Research in Marine Structures

    DEFF Research Database (Denmark)

    Soares, C.Guedes; Jensen, Jørgen Juncher; Incecik, A.

    2012-01-01

    An overview is presented of the results obtained in Europe by a network with a large number of research groups in the field of Marine Structures during a period of 6 years. The European Union has funded a project aimed at improving the collaboration among European research groups specialized...... in marine structures, which has led, among other results to a number of benchmark studies organized in 6 main topical areas, namely, Methods and Tools for Loads and Load Effects, Methods and Tools for Strength Assessment, Experimental Analysis of Structures, Materials and Fabrication of Structures, Methods...... and Tools for Structural Design and Optimization and Structural Reliability, Safety and Environmental Protection. This paper presents an overview of various studies performed, which helps identifying the level of consistency and robustness of different numeric tools used in this field....

  5. European Strategy for Astroparticle Physics

    Science.gov (United States)

    Katsanevas, Stavros

    2010-02-01

    Astroparticle Physics emerged worldwide, in the last 20 years, from a field of charismatic pioneers transgressing disciplinary frontiers using risky and innovative detection techniques to a full blown global science activity involving many hundreds of researchers and hundred million or billion dollar scale projects. I will report on the recent effort to develop a strategic vision, roadmap and action plan for this field in Europe, performed under the auspices of ApPEC (Astroparticle Physics European Coordination) and in the context of the European Union program ASPERA. I will describe the many links that tie the proposed large observatories to the corresponding US and more generally worldwide infrastructures, as well as the efforts for a global coordination in progress. )

  6. Integrating the European Securities Settlement

    Science.gov (United States)

    Schaper, Torsten

    The cross-border securities settlement in Europe is still said to be highly inefficient. One main reason can be seen in technical barriers between the different domestic settlement systems. Beside efforts to implement industry-specific communication standards an integration of the different settlement systems is necessary. The CSD-link model, the hub and spokes model, and the European CSD model aim to integrate European securities settlement. They have in common that they address the problem of interlinkage of national Central Securities Depositories and differ essentially in the way of achieving integration. These models are evaluated from a macro-economic perspective considering transaction costs, risks, and the integration of the cross-border securities settlement process.

  7. BEBC Big European Bubble Chamber

    CERN Multimedia

    CERN PhotoLab

    1974-01-01

    A view of the dismantling of the magnet of BEBC, the 3.7 m European Bubble Chamber : iron magnetic shielding ; lower and upper parts of the vacuum enclosure of the magnet; turbo-molecular vacuum pumps for the "fish-eye" windows; the two superconducting coils; a handling platform; the two cryostats suspended from the bar of the travelling crane which has a 170 ton carrying capacity. The chamber proper, not dismantled, is inside the shielding.

  8. The European Network of Uropathology

    DEFF Research Database (Denmark)

    Egevad, Lars; Algaba, Ferran; Berney, Dan M

    2009-01-01

    In pathology there is a need to rapidly disseminate professional information to the appropriate target groups. This is a surprisingly difficult task on an international level. Therefore, the European Network of Uropathology (ENUP) was recently organized by the Uropathology Working Group of the Eu...... conducted. Here we report on the methods used to organize this novel information network. We think that ENUP could serve as a model for other fields of pathology and other geographic regions....

  9. Turkey and European Security Institutions

    Science.gov (United States)

    2009-03-01

    relationships between Turkey, the United States, the Kurds, and Europe. As a source often critical of Turkey and the United States ( Noam Chomsky ...2008, http://consilium.europa.eu/uedocs/cmsUpload/080220Altheaupdate10. pdf . 129 Ibid. 130 Ibid. 131 Ibid. 132 The Council of the European Union...Factsheet, “EU Police Mission in Bosnia and Herzegovina (EUPM),” March 2006, http://consilium.europa.eu/uedocs/cmsUpload/Factsheet_060312. pdf . 48

  10. The European Digital Kitchen Project

    OpenAIRE

    Seedhouse, Paul; Preston, Anne; Olivier, Patrick; Dan, Jackson; Philip, Heslop; Madeline, Balaam; Rafiev, Ashur; Kipling, Matthew

    2014-01-01

    This article reports on the European Digital Kitchen, an EU-funded language learning project which promotes learning of languages, cultures and cuisines in digital interactive kitchens. The project involves taking a normal kitchen and specifically adapting it for language learning using the next generation of digital technology, namely activity recognition and sensor technology. We intend that learners will be able to learn aspects of the language whilst performing a meaningful real-world tas...

  11. European Missile Defense and Russia

    Science.gov (United States)

    2014-07-01

    Russian of meeting between then Prime Minister Putin and defence experts in Sarov, Russia , February 24, 2012. 64. Viktor Yesin and Yevgeniy Savostyanov... Putin Letter to Obama to Address Missile Defense, Security,” RIA-Novosti, May 15, 2013, available from en.rian.ru/ russia /20130515/181170446.html...UNITED STATES · ARMY WAR COLLEGE PRESS Carlisle Barrac , ’A STR KNG T H ..,... WI SDOM EUROPEAN MISSILE DEFENSE AND RUSSIA Keir Giles with

  12. [European perspectives of health telematics].

    Science.gov (United States)

    Rienhoff, O; Verhey, J

    2005-06-01

    The European perspectives of the present German developments in the field of health telematics are discussed critically. It is pointed out that technical projects have been financed with considerable means in the EU, however with out having any lasting effect on the value of health telematics in the health systems of Europe. A decisive cause is that the "health" topic was not codified in the Roman contracts. The international, global market is a crucial factor for international development and thus also for the orientation of the German projects and their economical and political success. The USA plays a dominant role on the global market. Caused by different reasons a corresponding market potential in the EU cannot be expected in the foreseeable future. With regard to the new options provided by telematics, it is therefore recommended that the national health services be reorganized, thus increasing quality and efficiency. With regard to progressively individualized medical care, the subject of "health" should afterwards be included in the European contracts. In the long run, an adjustment of the systems and uniform use of telematics could be achieved. Till then the coordination between the national governments in the area of health politics is seen as the most effective means for European integration.

  13. The accession of the European Union to the European Convention on Human Rights

    OpenAIRE

    Callewaert, Johan

    2014-01-01

    Provided for under the Treaty of Lisbon, the accession of the European Union to the European Convention on Human Rights is destined to be a landmark in European legal history because it will finally make it possible for individuals and undertakings to apply to the European Court of Human Rights for review of the acts of European Union institutions, which unquestionably play an increasingly important role in our daily lives. After nearly three years of negotiations, a draft agreement on Europe...

  14. Through Thick and Thin: ‘European Identification’ for a Justified and Legitimate European Union

    OpenAIRE

    Anna C. Davidson

    2008-01-01

    Debates on the viability of European integration often rest on the need for some form of common European identity. This article looks at European integration through the framework of normative political theory to explore what form of European identity is needed for the EU to be considered both justified – having a good or just reason for existence, and legitimate – having consent from its citizens. It critiques arguments for a purely justified EU, which rule out the need for a common European...

  15. Gendering European welfare states and citizenship

    DEFF Research Database (Denmark)

    Siim, Birte; Borchorst, Anette

    2017-01-01

    on gender, class, ethnicity or race, and nationality. One issue is what has been the effect of the European emphasis on women’s wage work and gender equality policies for women in different European countries? Another issue concerns how multiple discrimination is tackled and institutionalized in European......The chapter revisits the feminist scholarship on gendering of European welfare states and European citizenship, and reflects on the effects of globalization, Europeanization and migration. It first presents feminist perspectives on the liberal, the conservative and the social democratic welfare...... welfare states. A third issue is to what extent the Nordic welfare states still represent an attractive alternative model of social and gender equality to neo-liberalism. The final part discusses feminist approaches to reframe gender equality and gender justice from the transnational European contexts....

  16. European initiatives in postgraduate education in gerontology.

    Science.gov (United States)

    van Rijsselt, René J T; Parkatti, Terttu; Troisi, Joseph

    2007-01-01

    This paper describes three innovative European initiatives in postgraduate education in gerontology. The first is the European Masters Program in Gerontology (EuMaG), developed as an interdisciplinary joint program, supported and delivered by 22 European universities. Second, the Nordplus initiative to increase mobility of students and staff in the field of gerontology in the European Nordic countries is elaborated. Third, two postgraduate Gerontology and Geriatrics programs offered by the European Centre of Gerontology and Geriatrics, University of Malta are presented. In 1995, the Centre was designated a WHO Collaborating Centre for healthy aging. To provide a context for these initiatives, a short overview is presented of developments in the European Higher Education Area, and the current state and recent developments in gerontology training in Europe is elaborated. The article concludes with discussion of the feasibility and sustainability of European internationalization efforts in education and training in gerontology.

  17. European Union Energy Saving Policy

    Directory of Open Access Journals (Sweden)

    Nikolay Y. Kaveshnikov

    2014-01-01

    Full Text Available This article analyses methods of energy efficiency stimulation in the European Union. The author investigates basic areas of the EU activity; in particular, the author estimates results of implementation of the Energy Star program, new provisions of labeling of energy-using products, measures to increase energy efficiency in buildings. The paper also analyzes the provisions of the Directive 2012/27 that is the first EU document, providing for a comprehensive approach to energy saving at all stages: production, transformation and consumption. Today EU policy includes: 7 a general political and regulatory framework laid down by the European action plan on energy efficiency and Directive 2012/27; 2 national action plans on energy efficiency, which should be in line with indicative targets set at the EU level; 3 special EU documents in key areas of energy efficiency (buildings, energy-consuming equipment etc.; 4 accompanying instruments, such as target funding, information dissemination, support of specialized networks. The paper gives a comprehensive analysis of the key methods of implementation of EU policy in the area of energy saving. The author concludes that EU operates within the framework of the open method of coordination. The system of mandatory/voluntary technical standards has allowed to achieve significant success, but indicative planning and monitoring of national actions are not completely effective. In the long term EU policy in the area of energy efficiency is restrained by member states unwillingness to delegate to the European Union a more detailed powers in this field and to give the EU bodies facilities to execute more strict control. In the short term - in conditions of economic crisis, the EU countries are not ready to invest significant budget funds in projects with long payback period.

  18. Letter to European Science Foundation

    Directory of Open Access Journals (Sweden)

    2006-06-01

    Full Text Available Département de Linguistique AppliquéeEt de Didactique des languesDirecteur : Claude Springerhttp://dladl.u-strasbg.fr Dr. Rüdiger KleinEUROCORES Programme CoordinatorEuropean Science Foundation1, quai Lezay MarnésiaBP 90015F-67080 Strasbourg Cedex Strasbourg, le 22 mai 2006 Dear colleague, The Editorial Board of the journal ALSIC (Apprentissage des langues et système d’information et de communication which comprises 45 people from universities in 9 different countries was astonished to note...

  19. European perspectives of food safety.

    Science.gov (United States)

    Bánáti, Diána

    2014-08-01

    Food safety has been a growing concern among European Union (EU) citizens over the last decades. Despite the fact that food has never been safer, consumers are considerably uncertain and increasingly critical about the safety of their food. The introduction of new principles, such as the primary responsibility of producers, traceability, risk analysis, the separation of risk assessment and risk management provided a more transparent, science-based system in Europe, which can help to restore consumers' lost confidence. The present EU integrated approach to food safety 'from farm to fork' aims to assure a high level of food safety within the EU. © 2014 Society of Chemical Industry.

  20. The new European wind atlas

    DEFF Research Database (Denmark)

    Lundtang Petersen, Erik; Troen, Ib; Ejsing Jørgensen, Hans

    2014-01-01

    European Wind Atlas” aiming at reducing overall uncertainties in determining wind conditions; standing on three legs: A data bank from a series of intensive measuring campaigns; a thorough examination and redesign of the model chain from global, mesoscale to microscale models and creation of the wind atlas...... database. Although the project participants will come from the 27 member states it is envisioned that the project will be opened for global participation through test benches for model development and sharing of data – climatologically as well as experimental. Experiences from national wind atlases...... will be utilized, such as the Indian, the South African, the Finnish, the German, the Canadian atlases and others....

  1. popscience - European Researchers Night 2014

    CERN Multimedia

    Jeanneret, Guillaume

    2014-01-01

    Vendredi 26 septembre 2014, le CERN célèbrera la Nuit européenne des chercheurs à Genève et à St-Genis-Pouilly. Le thème de l’édition 2014 est inspiré d’Andy Warhol : « Pop science is for everyone ». On Friday 26 September 2014, CERN will be celebrating European Researchers' Night at three venues in Geneva and St. Genis-Pouilly. Inspired by Andy Warhol, this year's theme is “Pop science is for everyone”.

  2. The early European lithium studies.

    Science.gov (United States)

    Schou, M

    1999-12-01

    Cade's discovery of lithium's antimanic effect soon became known in Europe and was confirmed by a Danish controlled trial. The same investigators discovered a prophylactic action of lithium against both manic and depressive recurrences, which was confirmed by a Swiss-Czech-Danish cooperative trial. The evidence of these studies was met with skepticism based on methodological speculations, but a Danish randomised, placebo-controlled, double-blind trial of discontinuation design established the prophylactic action of lithium, as did a number of European controlled trials of start design and discontinuation design. The review ends with personal memories of John Cade.

  3. Welfare reform in European countries

    DEFF Research Database (Denmark)

    Immervoll, Herwig; Kleven, Henrik Jacobsen; Kreiner, Claus Thustrup

    2007-01-01

    microsimulation model to estimate current marginal and participation tax rates. We quantify the equity-efficiency trade-off for a range of elasticity parameters. In most countries, because of large existing welfare programmes with high phase-out rates, increasing traditional welfare is undesirable unless......This article compares the effects of increasing traditional welfare to introducing in-work benefits in the 15 (pre-enlargement) countries of the European Union. We use a labour supply model encompassing responses to taxes and transfers along both the intensive and extensive margins, and the EUROMOD...

  4. Intersectionality in European Union policymaking

    DEFF Research Database (Denmark)

    Lombardo, Emanuela; Agustin, Lise Rolandsen

    2016-01-01

    is particularly apt to deal with equality and diversity in policymaking. By analysing a selection of European Union policy documents on gender-based violence in the period 2000–2014, we attend to the question of what intersectionality can bring to policymaking in terms of strengthening inclusiveness and address......Inclusiveness of different social groups and responsiveness to the needs of increasingly diverse societies are key criteria for policy analysts to assess the quality of public policies. We argue that an intersectional approach attentive to the interaction of gender with other inequalities...

  5. European ecological networks and greenways

    DEFF Research Database (Denmark)

    Kristiansen, Ib; Jongman, Rob H.G.; Kulvik, Mart

    2004-01-01

    In the context of European integration, networks are becoming increasingly important in both social and ecological sense. Since the beginning of the 1990s, societal and scientific exchanges are being restructured as the conceptual approaches towards new nature conservation strategies have been...... renewed. Within the framework of nature conservation, the notion of an ecological network has become increasingly important. Throughout Europe, regional and national approaches are in different phases of development, which are all based on recent landscape ecological principles. Ecological networks....... This complex interaction between cultural and natural features results in quite different ways for the elaboration of ecological networks and greenways....

  6. An important day for European science

    CERN Multimedia

    2009-01-01

    Today is an important day for the future of European science. It is the day on which CERN’s relationship with the European Union reaches maturity. Ever since the early 1950s, CERN and the European project have grown together. CERN was a child of the European movement: the first discussions at Denis de Rougemont’s European Cultural Conference in Lausanne in 1949 led directly to the foundation of CERN in 1954. More recently, the European Union has become an Observer at the CERN Council, links between CERN and the EU have strengthened as Europe has pursued the goal of creating a European Research Area, and CERN has benefited from European support for projects as varied as Grid computing and future accelerator R&D, not forgetting, of course, the numerous Marie Curie fellows whose careers have received a boost thanks to European funds. Today, that collaboration goes one step further with the signature of a Memorandum of Understanding between CERN and the European C...

  7. The European-American exchange.

    Science.gov (United States)

    Guerra, F

    1993-01-01

    The European-American exchange of infectious diseases was responsible for the demographic havoc of the native population in the New World after 1492. Prior to this date medical writers describe the presence in Spain of viral diseases like influenza, parotitis, smallpox, measles, poliomyelitis, and rabies; there were also rickettsiasis, diphtheria, salmonellosis, plague, tubercolosis, leprosy, malaria, scabies and tinea. In America, before European arrivals, there were no records of human viral diseases, though there were records of rickettsiasis, treponematosis--pinta, yaws and syphilis--leihsmaniasis, amibiasis and perhaps leprosy. With the discovery of America in 1492, Columbus's sailors were contaminated by yaws and spread this disease into Europe. In 1493 influenza, as a zoonosis, was introduced into Santo Domingo and was responsible for the annihilation of the natives of the Antilles in less than a quarter of a century; in 1518 smallpox was also introduced in Santo Domingo and then to the American continent by negro slaves: by the same means measles were introduced in 1531. The previous existence or introduction of other infectious diseases in America is also discussed.

  8. ALARA in European nuclear installations

    Energy Technology Data Exchange (ETDEWEB)

    Lefaure, C. [CEPN, Fontenay-Aux-Roses (France); Croft, J. [NRPB, Chilton, Didcot (United Kingdom); Pfeffer, W. [GRS, Koeln (Germany); Zeevaert, T. [SCK/CEN, Mol (Belgium)

    1995-03-01

    For over a decade the Commission of the European Community has sponsored research projects on the development and practical implementation of the Optimization principle, or as it is often referred to, ALARA. These projects have given rise to a series of successful international Optimization training courses and have provided a significant input to the periodic European Seminars on Optimization, the last one of which took place in April 1993. This paper reviews the approaches to Optimization that have development within Europe and describes the areas of work in the current project. The on-going CEC research project addresses the problem of ALARA and internal exposures, and tries to define procedures for ALARA implementation, taking account of the perception of the hazard as well as the levels of probability of exposure. The relationships between ALARA and work management, and ALARA and decommissioning of installations appear to be other fruitful research areas. Finally, this paper introduces some software for using ALARA decision aiding techniques and databases containing feed back experience developed in Europe.

  9. Citizenship Education Development: European Experience

    Directory of Open Access Journals (Sweden)

    A. V. Suslov

    2012-01-01

    Full Text Available The paper considers the European experience of the citizenship education developmentan important aspect of internal policy in the most countries. The education in question is considered to be the democratic citizenship training aimed at developing the loyal attitude to different value priorities in society, social responsibility, active citizenship position, awareness of democratic rights, capability of using and protecting them.The author looks at the transformation of citizenship education concept in the last three decades from the civics education (i.e. history, political science, law, etc. to the democratic citizen education. The paper analyzes differ- ent approaches to citizenship education in several European countries including the post-soviet ones. It is emphasized that both in western and eastern Europe a lot of effort is made for spreading and supporting the education in question. The author recommends considering the foreign experience of integrating the democratic citizenship education into the state academic curricula at the primary, secondary and higher school levels. 

  10. European Integration and Labour Migration

    Directory of Open Access Journals (Sweden)

    Julda Kielyte

    2010-11-01

    Full Text Available The present paper studies how European integration might affect the migration of workers in the enlarged EU. Unlike the reduced-form migration models, we base our empirical analysis on the theory of economic geography à la Krugman (1991, which provides an alternative modelling of migration pull and push factors. Parameters of the theoretical model are estimated econometrically using historical migration data. Our empirical findings suggest that European integration would trigger selective migration between the countries in the enlarged EU. In the Baltics, Lithuania would gain about 7.25% of the total work force. In the Visegrád Four, the share of the mobile labour force would increase the most in Hungary, 8.35%, compared to the pre-integration state. Our predictions for the East-West migration are moderate and lower than those of reduced-form models: between 5.44% (from the Baltics and 3.61% (from the Visegrád Four would emigrate to the EU North. Because migrants not only follow market potential, but also shape the region’s market potential, the long-run agglomeration forces are sufficiently weak to make a swift emergence of a core-periphery pattern in the enlarged EU very unlikely.

  11. Policymaking in European healthy cities.

    Science.gov (United States)

    de Leeuw, Evelyne; Green, Geoff; Spanswick, Lucy; Palmer, Nicola

    2015-06-01

    This paper assesses policy development in, with and for Healthy Cities in the European Region of the World Health Organization. Materials for the assessment were sourced through case studies, a questionnaire and statistical databases. They were compiled in a realist synthesis methodology, applying theory-based evaluation principles. Non-response analyses were applied to ascertain the degree of representatives of the high response rates for the entire network of Healthy Cities in Europe. Further measures of reliability and validity were applied, and it was found that our material was indicative of the entire network. European Healthy Cities are successful in developing local health policy across many sectors within and outside government. They were also successful in addressing 'wicked' problems around equity, governance and participation in themes such as Healthy Urban Planning. It appears that strong local leadership for policy change is driven by international collaboration and the stewardship of the World Health Organization. The processes enacted by WHO, structuring membership of the Healthy City Network (designation) and the guidance on particular themes, are identified as being important for the success of local policy development. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  12. Europeanization and social movement mobilization during the European sovereign debt crisis

    DEFF Research Database (Denmark)

    Bourne, Angela; Chatzopoulou, Sevasti

    2015-01-01

    The article addresses Europeanization of social movements in the context of the European Sovereign Debt Crisis. Europeanization occurs when movements collaborate, or make horizontal communicative linkages with movements in other countries, contest authorities beyond the state, frame issues...... as European and claim a European identity. The article presents a theoretical framework and research design for measuring the degree of social movement Europeanization followed by results of a pilot study on mobilization in Spain and Greece during 2011. While many contentious action events studied only...

  13. Possible causes for growth variability and summer growth reduction in juvenile plaice Pleuronectes platessa L. in the western Dutch Wadden Sea

    Science.gov (United States)

    van der Veer, Henk W.; Jung, Alexa Sarina; Freitas, Vânia; Philippart, Catharina J. M.; Witte, Johannes IJ.

    2016-05-01

    Growth variability within individuals and among groups and locations and the phenomenon of summer growth reduction has been described for juvenile flatfish in a variety of European coastal areas whereby the underlying causes still remain elusive. Potential mechanisms were tested for juvenile plaice Pleuronectes platessa L. in the western Dutch Wadden Sea, by analysing published and unpublished information from long-term investigations (1986-present). Growth variability did occur and could be explained by differences induced by environmental variability (water temperature), and by non-genetic irreversible adaptation and sex. Dynamic Energy Budget analysis indicated that especially sexually-dimorphic growth in combination with variability in sex ratio could explain most of the variability in growth and the increase in the range of the size of individuals within the population over time. Summer growth reduction was not only observed among 0-group plaice in the intertidal, but also in the subtidal and tidal gullies as well as among I- and II-group plaice. Intraspecific competition for food was not detected but some support for interspecific competition with other predators was found. Also resource competition (due to crowding) with the other abundant epibenthic species (0-, I- and II-group flounder Platichthys flesus; the brown shrimp Crangon crangon; the shore crab Carcinus maenas; the goby species Pomatoschistus minutus and Pomatoschistus microps) could not explain the summer growth reduction. The observed growth reduction coincided with a decrease in stomach content, especially of regenerating body parts of benthic prey items. It is hypothesised that macrozoobenthos becomes less active after the spring phytoplankton bloom, reducing prey availability for juvenile plaice in summer, causing a reduction in food intake and hence in growth.

  14. The European Public on Social Media

    DEFF Research Database (Denmark)

    Tarta, Ancuta-Gabriela

    This study explorers the European public on social media by discussing the case of communicative interactions taking place on the official Facebook page of the European Parliament. Based on the theoretical framework conceptualizing the public and the public sphere on social network sites, the study...... examines the European social media public from two perspectives. The first is a top-down, institutional perspective of European Parliament, based on a case-study approach to the way this European institution constructs and addresses the public through its social media communication. The second is a bottom......-up perspective of the public of social media users, informed by a content analysis of Facebook comments and a discourse analysis of live chat interactions with Members of the European Parliament. Results confirm a discursive potential of the Facebook public of users. In the case of the Facebook comment threads...

  15. THE EUROPEAN COMMUNITY PRIORITY - THE SOCIAL POLICY

    Directory of Open Access Journals (Sweden)

    Gina Ignat

    2009-12-01

    Full Text Available At the level of European Union, starting from the necessity of insurance economic growth, social community law gradually developed, benefitting by sinuous construction, extremely complex, beingcontinuously transformed. Familiarization with the model of social community law prescribed by European Union represents not only a forward step in the process of harmonization with community law, but also an alignment in settlement of European Union, which finally establishes the modernization of the whole domestic law system.

  16. Is there a European medical sociology?

    OpenAIRE

    Henckes, Nicolas; Baszanger, Isabelle

    2014-01-01

    International audience; Is there a European sociology of health, illness and medicine – or, in short, a European medical sociology? While the answer to this question would certainly have been negative just a few years ago, this chapter argues that over the last thirty years a series of new analyses have emerged in the field which have laid the groundwork for a medical sociology at the European level. These examine how health issues are increasingly framed as consumer goods, public issues and ...

  17. European Union Financial Crisis: a Marxist Analysis

    OpenAIRE

    Farneubun, Petrus K.

    2016-01-01

    European financial crisis poses a serious challenge to the fundamental structure of the European Union, political and financial institutions, as well as the values that bind European together. Different factors have been suggested as the causes of the crisis notably the failure of national government and economic policies. Responding to the crisis, numerous attemps have been proposed to accelerate the recovery, but the crisis still hit Eurozone and brought serious consequences politically and...

  18. Book Review: Diversity in European Marketing

    Directory of Open Access Journals (Sweden)

    Hana Machková

    2012-06-01

    Full Text Available The case book “Diversity in European Marketing” is the result of the scientific cooperation of the CEMS Marketing Faculty Group. Prestigious Central European Universities - Corvinius University of Budapest and University of Economic, Prague - are members of CEMS - the Global Alliance in Management Education of 26 leading business schools and 70 multinational companies. Four authors from these two Universities share in this book their experience of Central European Marketing specifics.

  19. The European Boidiversity Observation Network - EBONE

    OpenAIRE

    Halada, L.; Jongman, R.H.G.; Gerard, F; Whittaker, L.; R. G. H. Bunce; Bauch, B.; Schmeller, D. S.

    2009-01-01

    EBONE (European Biodiversity Observation Network) is a project developing a system of biodiversity observation at regional, national and European levels as a contribution to European reporting on biodiversity. The project focuses on GEO (Group of Earth Observations) task BI 07-01 to unify many of the disparate biodiversity observation systems and creates a platform to integrate biodiversity data with other types of information. The system will make use of existing networks of site observation...

  20. Theory and reform in the European Union

    OpenAIRE

    Chryssochoou, Dimitris N.; Tsinisizelis, Michael J.; Stavridis, Stelios; Ifantis, Kostas

    2003-01-01

    This substantially updated and revised edition offers a comprehensive overview of the challenges confronting the political system as well as the international politics of the European Union. It draws from a rich spectrum of regional integration theories to determine what the Union actually is and how it is developing. The book examines constitutional politics of the European Union, from the Single European Act to the Treaty of Nice and beyond. The ongoing debate on the future of Europe links ...

  1. Fiscal Convergence in an Enlarged European Union

    Directory of Open Access Journals (Sweden)

    Georgeta VINTILĂ

    2014-02-01

    Full Text Available Taxation convergence in the European Union has always been a heatedly debated issue, and it has constantly resurfaced to the limelight as more and more fiscal directive proposals are being discussed. The current study is an analysis of the evolution of taxation convergence tendencies within the European Union in recent years, using sigma-convergence and cluster analysis. Our goal is to analyze the fiscal convergence trends in the context of European Union enlargement over the last two decades.

  2. The spatialization of the european project

    DEFF Research Database (Denmark)

    Richardson, Tim; Jensen, Ole B.

    This paper argues the need for more critical analysis of the ?spatialisation of the European Project? than has been the case in much previous academic work on the European Union`s spatial policy so far. The focus is on the EU, and the way that this huge institutional and territorial setting...... is organised, driven and imagined according to a particular hegemonic spatiality which gives shape to the political, economic, social and territorial dimensions of the European project....

  3. Developments in international/European health law.

    Science.gov (United States)

    Abbing, Henriette D C Roscam

    2009-03-01

    International (European) organizations have impact on health law. The most recent developments are: a revision of the world Medical's Association Declaration of Helsinki, a proposal for a Directive (European Commission) on standards of quality and safety of human organs intended for transplantation, accompanied by a ten point action plan; a proposal (European Commission) for a Directive on the application of patients' rights in cross-border health care; a proposal (European commission) for a Directive on information to the general public on medicinal products subject to medical prescription.

  4. European Identity between Ethnic and Civic Identities

    Directory of Open Access Journals (Sweden)

    Paul Kun

    2015-11-01

    Full Text Available The European identity is not a reality, but a necessity for a stabile future of the European political construction. The democratic deficit problem results from the fact that the European project was conceived as a top-down type of action. Its legitimization is however a bottom-up process. For this reason, the institutional project needs to be supported by an ideological project for a European identity. There are two ways, two different patterns for the second charge: the ethnic-cultural identity or the civic identity. Each has his advantages, but also disadvantages. This paper analyzes the results of one sociological research among young Romanian students.

  5. Federal and European Union Policy Making

    DEFF Research Database (Denmark)

    Dosenrode, Søren

    By using the policy cycle approach, this paper compares the policy-process in a number of Anglo-Saxon type federations with the policy-process in continental-European style federations from within the European Union (EU) area.  The comparison would reveal: a) distinct different styles of policy...... making in European style federations in relation with the Anglo-Saxon ones. b) that the policy-process in the EU resembles that of the European style federations, and c) the Constitutional Treaty (CT) or a possible CT-light would strengthen the federal policy-making characteristics already present...

  6. European Union Climate Change Objectives

    Energy Technology Data Exchange (ETDEWEB)

    Lewiner, C.

    2007-08-15

    In March 2007, the European Union Ministers asked Member States to commit to energy consumption and Greenhouse Gases (GHG), mainly CO2 emissions, reductions of 20%, as well as a portion of 20% of renewable energies in their energy production. The horizon of this 'three times 20% objective' is 2020. The underlying assumption is of course an improved security of energy (and electricity) supplies as well as a growing European economy with sustained tertiary and industrial employment. These objectives were articulated to respond to the present Climate Change issues by avoiding an increase of our Planet's temperature beyond 2 to 3 degrees. In the US, the current government has recognized that CO2 is an issue and has begun to take action, issuing a number of executive orders to reduce emissions. This follows a ruling by the US Supreme Court on CO2. It may be that what is happening in Europe will be a model that the US will follow. These different objectives are interlinked and each European country is in a different situation. Moreover, 2020 is a relatively short time horizon compared to the lead time for construction of large plants such as nuclear plants. It is also a short time frame for the industrialization, at reasonable costs, of carbon sequestration equipment, for the renovation of a significant portion of the existing buildings and houses, and for the switch of the present car fleet to electrical cars. More than 20% of the cars bought in 2007 will still be on the road in 2020. More than 80% of the refrigerators bought in 2007 will still be in operation in 2020, and less than 1/3 of the industrial and utility infrastructures in place today will be renewed by 2020. It is even shorter at the Research and Development time scale to find and industrialize new energy savings devices or low energy consumption and low carbon emitting equipment. One important question is also the net cost linked to these objectives that has not been evaluated today. The

  7. Training of European urology residents in laparoscopy: results of a pan-European survey

    NARCIS (Netherlands)

    Furriel, Frederico T. G.; Laguna, Maria P.; Figueiredo, Arnaldo J. C.; Nunes, Pedro T. C.; Rassweiler, Jens J.

    2013-01-01

    To assess the participation of European urology residents in urological laparoscopy, their training patterns and facilities available in European Urology Departments. A survey, consisting of 23 questions concerning laparoscopic training, was published online as well as distributed on paper, during

  8. THE CONSOLIDATION OF EU GOVERNANCE: THE EUROPEAN SEMESTER AND THE EVALUATION OF EUROPEAN INTEGRATION DEEPENING

    Directory of Open Access Journals (Sweden)

    Nicolae TODERAS

    2016-03-01

    Full Text Available Once it was introduced as an instrument for deepening European integration, the legitimacy, opportunity and utility of European Semester were repeatedly questioned from the perspective of its contribution to the institutionalization of European governance. Also, there is a strong and direct link between the further deepening of the European Union’s political and economic integration on the one hand, and the development of a culture of evaluating policies and programs on the other. Taking these aspects into account, the following questions arise: does the European Commission have, in its position as manager of the European Semester, a real capacity of evaluating all aspects regarding the deepening of European integration? Can the European Semester be a means for diffusing evaluation practice at the level of Member States? This is why, in this paper, we propose to give answers to these questions.

  9. Determinação de idades e crescimento do linguado branco Paralichthys patagonicus (Jordan, 1889 no sul do Brasil Age and growth of the white flounder Paralichthys patagonicus (Jordan, 1889 in southern Brazil

    Directory of Open Access Journals (Sweden)

    Júlio Neves de Araújo

    2000-01-01

    Full Text Available A idade e o crescimento do linguado-branco Paralichthys patagonicus (Jordan, 1889 foram estudados a partir de amostras obtidas da pesca comercial no sul do Brasil entre 1989 e 1991. As idades foram determinadas sobre cortes transversais de otólitos de 460 exemplares. A análise do tipo de bordo e dos incrementos marginais dos otólitos mostrou que, a cada ano ocorre a formação de uma zona translúcida e uma zona opaca nos otólitos. As "remeas atingem maiores tamanhos e idades (630 mm, 13 anos do que os machos (527 mm, 11 anos. Os parâmetros da equação de von Bertalanffy que representaram o crescimento em comprimento total das remeas foram: CT∞,= 623,0 mm, K= 0,279 e to= -0,243 anos; dos machos: CT∞,= 534,1 mm, K= 0,214 e t o = -1,589 anos e dos sexos agrupados: CT∞, = 609,1 mm, K = 0,255 to = - 0,462 anos. Os incrementos anuais em peso por idades aumentam até o final do quarto ano de vida quando a maioria já atingiu a primeira maturação sexual e ambos sexos alcançaram cerca de 30% do peso e 70% do comprimento assintóticos. Esta espécie é susceptível à sobrepesca de crescimento porque o redirecionamento do metabolismo do crescimento somático para a reprodução é gradativo e o potencial de crescimento após a maturação sexual é relativamente alto.Age and growth of the white flounder Paralichthys patagonicus (Jordan, 1889 were studied using sliced sagittae otoliths of 460 specimens collected from the commercial fishery along southem Brazil (1989 to 1991. The changes in the type of last band in formation and the marginal increments analysis showed that one translucent and one opaque zone were formed each year. Females attained larger totallength (TL and older ages (630 mm, 13 yr than males (527 mm, 11 yr. The parameters ofthe von Bertalanffy growth equations were: for females - TL∞,= 623.0 mm, K= 0.279 and t o = - 0.243 yr; for males - TL∞,= 534.1 mm, K= 0.214, to = - 1.589 yr and for pooled sexes - TL∞, = 609.1 mm

  10. European perspectives on security research

    CERN Document Server

    2011-01-01

    Europe’s networked societies of today are shaped by a growing interconnection in almost all areas of life. The complexity of our infrastructures and the concurrent accessibility to means of destruction by terrorist groups and individual perpetrators call for innovative security solutions. However, such evolving innovations inevitably raise fundamental questions of concern in our societies. How do we balance the imperatives of securing our citizens and infrastructures on the one hand, and of protecting of our sacredly held civil liberties on the other? The topical network ‘Safety and Security’ of acatech – the German Academy of Science and Engineering – invited experts from the science academies of various European countries to share their perspectives on security research and the aspect of safety during a two-day workshop hosted by the Fraunhofer Institute for High-Speed Dynamics, Ernst-Mach-Institut in March 2010. This publication is a compilation of contributions made during the workshop.

  11. Reindustrialization Process in European Union

    Directory of Open Access Journals (Sweden)

    Petre Prisecaru

    2014-04-01

    Full Text Available In order to support the economic growth and empolyment in EU European institutions have launches ambitious initiatives for a reindustrialization process based on new technologies and green energies. The aim is to increase the share of processing industry in GDP from 15.1% in 2013 to 20% in 2020 according to the objectives of Europe 2020 Strategy and also with those of other EU strategies and common policies. The focus will be laid on innovation and research activites, on supporting/financing SMEs and their internationalization, on the integration of EU firms into global value chains, on developing green energies, bio and key enabling technologies, on smart networks and on free trade and sustainable development.

  12. The European perspective for LSST

    Science.gov (United States)

    Gangler, Emmanuel

    2017-06-01

    LSST is a next generation telescope that will produce an unprecedented data flow. The project goal is to deliver data products such as images and catalogs thus enabling scientific analysis for a wide community of users. As a large scale survey, LSST data will be complementary with other facilities in a wide range of scientific domains, including data from ESA or ESO. European countries have invested in LSST since 2007, in the construction of the camera as well as in the computing effort. This latter will be instrumental in designing the next step: how to distribute LSST data to Europe. Astroinformatics challenges for LSST indeed includes not only the analysis of LSST big data, but also the practical efficiency of the data access.

  13. European environmental policy: The pioneers

    DEFF Research Database (Denmark)

    Andersen and Liefferink's edited volume takes a different approach to comparative environmental policy studies. It looks at how domestic environmental policies and activities In six specific countries (Sweden, Austria, Finland, Germany, The Netherlands, Denmark and Norway) have affected environme......Andersen and Liefferink's edited volume takes a different approach to comparative environmental policy studies. It looks at how domestic environmental policies and activities In six specific countries (Sweden, Austria, Finland, Germany, The Netherlands, Denmark and Norway) have affected...... environmental politics at the European Union level. Following Robert Putnam's theory, environmental policymaking in the EU is viewed as a reciprocal two-level game in which activities, actors and politics in domestic and EU arenas affect each other. Governments at times need to build domestic political support...

  14. European Atlas of Soil Biodiversity

    DEFF Research Database (Denmark)

    Krogh (contributor), Paul Henning

    a normal atlas. Produced by leading soil scientists from Europe and other parts of the world under the auspice of the International Year of Biodiversity 2010, this unique document presents an interpretation of an often neglected biome that surrounds and affects us all. The European Atlas of Soil...... world. The first part of the book provides an overview of the below ground environment, soil biota in general, the ecosystem functions that soil organism perform, the important value it has for human activities and relevance for global biogeochemical cycles. The second part is an 'Encyclopedia of Soil...... Biodiversity is an essential reference to the many and varied aspects of soil. The overall goal of this work is to convey the fundamental necessity to safeguard soil biodiversity in order to guarantee life on this planet....

  15. BANKING SUPERVISION IN EUROPEAN UNION

    Directory of Open Access Journals (Sweden)

    Lavinia Mihaela GUȚU

    2013-10-01

    Full Text Available The need for prudential supervision imposed to banks by law arises from the action that banking market’s basic factors have. Therefore, it is about banks’ role in economy. The normal functioning of banks in all their important duties maintains the stability of banking system. Further, the stability of the entire economy depends on the stability of the banking system. Under conditions of imbalance regarding treasury or liquidity, banks are faced with unmanageable crisis and the consequences can be fatal. To ensure long-term stability of the banking system, supervisory regulations were constituted in order to prevent banks focusing on achieving rapidly high profits and protect the interests of depositors. Starting from this point, this paper will carry out a study on existing models of supervision in the European Union’s Member States. A comparison between them will support identifying the advantages and disadvantages of each of them.

  16. European Conference on Data Analysis

    CERN Document Server

    Krolak-Schwerdt, Sabine; Böhmer, Matthias; Data Science, Learning by Latent Structures, and Knowledge Discovery; ECDA 2013

    2015-01-01

    This volume comprises papers dedicated to data science and the extraction of knowledge from many types of data: structural, quantitative, or statistical approaches for the analysis of data; advances in classification, clustering, and pattern recognition methods; strategies for modeling complex data and mining large data sets; applications of advanced methods in specific domains of practice. The contributions offer interesting applications to various disciplines such as psychology, biology, medical and health sciences; economics, marketing, banking, and finance; engineering; geography and geology;  archeology, sociology, educational sciences, linguistics, and musicology; library science. The book contains the selected and peer-reviewed papers presented during the European Conference on Data Analysis (ECDA 2013) which was jointly held by the German Classification Society (GfKl) and the French-speaking Classification Society (SFC) in July 2013 at the University of Luxembourg.

  17. European Union: Gender and politics

    Directory of Open Access Journals (Sweden)

    Žunić Natalija

    2014-01-01

    Full Text Available Political representation is the central issue in contemporary debates on the level of democracy in political institutions and processes in the European Union. Underrepresentation of particular groups in political institutions, decision-making and policy-making processes is perceived as the problem of justice, legitimacy and effectiveness in democratic societies. In this paper, the author analyzes the gender aspects of democratic decision-making processes and political representation of women in the EU member states. The social, historical and political dimension of women's efforts to obtain and promote their civil status and political rights have been the framework for developing the principle of gender equality as one of the founding EU principles. In the past hundred years, one of the most significant trends in politics has been the expansion of formal political representation of women. Yet, even though it has been more than a hundered years since women won their political rights in the 19th and the 20th century (the right to vote and the right to be voted, gender differences in political rights are still a substantial part of debate. Today, women's political representation is still inadequate and their political capacity and power have not been exercised to a sufficient extent (or proportionally through their actual representation in parliament. In March 2012, the European Commisision published a report on gender equality in different areas of social life; the Eurobarometer survey shows that women are generally underrepresented in politics. In national parliaments, only one out of four MPs is a woman. In the European Parliament, three out of ten parliamentarians are women. The statistics shows a huge discrepancy among the EU Member States in terms of women's representation in parliament (44.7% in Sweden as contrasted to 13.3% in Romania. The prevailing view in many studies is that post-industrial democracies are deficient as they have failed

  18. When Culture Hampers European Integration

    DEFF Research Database (Denmark)

    Juul Petersen, Jeppe

    2016-01-01

    This article deals with Denmark’s skeptical attitude towards the EU cooperation. From a hermeneutical-institutional approach the aim of this article is to analyze why Denmark has been rejecting several initiatives from the EU. It illustrates how different democratic understandings hamper European...... integration. Based on Ronald Dworkin’s theoretical framework the article discusses two different perceptions on democracy: majoritarian democracy and constitutional democracy. It is shown when and why EU’s member states prefer to handle EU-related judicial disputes without involving supranational institutions....... In addition, the article provides tentative comparisons to Britain and the Nordic countries since they show similar attitudes to supranational institutions. The article concludes that Denmark’s majoritarian democracy provides political cultures incompatible with the EU’s constitutional democracy and this can...

  19. Kant's Theory of European Integration

    DEFF Research Database (Denmark)

    Harste, Gorm

      Abstract:Immanuel Kant developed a rather wide range of concepts to describe the process of an "ever broader union" of European states. Kant's theory posed a dilemma with two aspects. On the one hand republican states were free, and on the other they were obliged to follow rules of a union...... of states, for instance regulations of non-intervention. Debates have focused this dilemma as if it was a real contradiction. The argument in the present paper is that there is no such contradiction. To the contrary, in his philosophy of reflective judgement, Kant describes the compelling duty......, but to let it cooperate with other states rather than to isolate it. Kant observes a guarantee that such a form of integration could emerge and succeed, because the military powers of Europe had to develop complexities of separated powers in their organisation of states. Underneath the unity of isolated...

  20. INNOVATION IN THE EUROPEAN UNION

    Directory of Open Access Journals (Sweden)

    VLADIMIR-CODRIN IONESCU

    2014-11-01

    Full Text Available In the contemporary knowledge-based society, innovation, as a vector used for the application and promotion of inventions and innovations, is one of the main sources used for obtaining a sustainable competitive advantage. The present paper points out the main directions which the European Commission aims to develop in the sphere of innovation, within the context of the Initiative: “A Union of Innovation”, which is part of the Europe 2020 Strategy. The paper analyses the performances accomplished by the EU states in the area of innovation while developing an international perspective that may represent the starting point in identifying solutions whereby the EU could reduce the gaps that exist in relation to the main international competitors, i.e. South Korea, USA and Japan.

  1. [AIDS prevention in European prisons].

    Science.gov (United States)

    Schaller, G; Harding, T W

    1995-01-01

    Informations on HIV/AIDS in prisons which include 32 prison-systems in 17 European countries have been collected. The principle of equivalence of preventive measures and health care between prisons and the outside community, prison authorities should strive to prevent HIV transmission in prisons. There have been major developments in effective prevention programmes in the community, there has been less progress within the prison-systems. Information on AIDS is provided to prisoners in almost all prison-systems. While condom distribution is practiced in a growing number of systems, no prison systems have adopted syringes/needle distribution or exchange during incarceration. A few system have accepted distribution of a disinfectant (diluted bleach) with instruction on cleaning injection materials. Despite clear recommendations made by international bodies in 1987/88, it must be concluded that discrimination and segregation (restriction from access to workshops, specially work in kitchens) remain widespread in 1992.

  2. European Conference on Complex Systems

    CERN Document Server

    Pellegrini, Francesco; Caldarelli, Guido; Merelli, Emanuela

    2016-01-01

    This work contains a stringent selection of extended contributions presented at the meeting of 2014 and its satellite meetings, reflecting scope, diversity and richness of research areas in the field, both fundamental and applied. The ECCS meeting, held under the patronage of the Complex Systems Society, is an annual event that has become the leading European conference devoted to complexity science. It offers cutting edge research and unique opportunities to study novel scientific approaches in a multitude of application areas. ECCS'14, its eleventh occurrence, took place in Lucca, Italy. It gathered some 650 scholars representing a wide range of topics relating to complex systems research, with emphasis on interdisciplinary approaches. The editors are among the best specialists in the area. The book is of great interest to scientists, researchers and graduate students in complexity, complex systems and networks.

  3. Impressions of European Integration : A comparative analysis of the impact of EU news in European newspapers on European audiences

    NARCIS (Netherlands)

    't Lam, P.A.

    2012-01-01

    This study demonstrates that the coverage of the EU in the European press affects the knowledge and attitude towards the EU of the average European newspaper reader. This outcome is the more relevant now that the EU is steadily growing in size and impact, but conversely is confronted with a general

  4. PRACE - The European HPC Infrastructure

    Science.gov (United States)

    Stadelmeyer, Peter

    2014-05-01

    The mission of PRACE (Partnership for Advanced Computing in Europe) is to enable high impact scientific discovery and engineering research and development across all disciplines to enhance European competitiveness for the benefit of society. PRACE seeks to realize this mission by offering world class computing and data management resources and services through a peer review process. This talk gives a general overview about PRACE and the PRACE research infrastructure (RI). PRACE is established as an international not-for-profit association and the PRACE RI is a pan-European supercomputing infrastructure which offers access to computing and data management resources at partner sites distributed throughout Europe. Besides a short summary about the organization, history, and activities of PRACE, it is explained how scientists and researchers from academia and industry from around the world can access PRACE systems and which education and training activities are offered by PRACE. The overview also contains a selection of PRACE contributions to societal challenges and ongoing activities. Examples of the latter are beside others petascaling, application benchmark suite, best practice guides for efficient use of key architectures, application enabling / scaling, new programming models, and industrial applications. The Partnership for Advanced Computing in Europe (PRACE) is an international non-profit association with its seat in Brussels. The PRACE Research Infrastructure provides a persistent world-class high performance computing service for scientists and researchers from academia and industry in Europe. The computer systems and their operations accessible through PRACE are provided by 4 PRACE members (BSC representing Spain, CINECA representing Italy, GCS representing Germany and GENCI representing France). The Implementation Phase of PRACE receives funding from the EU's Seventh Framework Programme (FP7/2007-2013) under grant agreements RI-261557, RI-283493 and RI

  5. Nutritional risk of European elderly.

    Science.gov (United States)

    de Morais, C; Oliveira, B; Afonso, C; Lumbers, M; Raats, M; de Almeida, M D V

    2013-11-01

    The elderly constitute a population group with a high prevalence of non-communicable chronic diseases and high risk of malnutrition. The aim of this study was to identify factors associated to nutritional risk in free-living European elderly. The sample included 644 European citizens, free living in the community, aged 65 years or more. The sample was quota controlled for age groups (65-74, ≥75 years), gender (male/female) and living circumstances (living alone/with others). Logistic regression was performed to identify factors associated with nutritional risk. Several variables regarding socio-demographic characteristics, food choice, health status and the satisfaction with food-related life were included in the analysis. According to the recoded score of the 'Determine your nutritional health' (NSI checklist), 53% of the elderly were at nutritional risk. Nutritional risk was more likely to occur in elderly who considered that it was more important to choose foods 'easy to chew'; with lower average number of fruit and vegetables (F&V) intake episodes and lower score for general health. It was also found in non-married participants; those that did not identify changes in their appetite; and those that felt changes in health status. In this sample, the lowest nutritional risk was found for body mass index (BMI) around 18.5 kg/m(2). Country of residence, gender and age were not found to have a significant effect on nutritional risk. Attention should be drawn to the living circumstances, changes in appetite or health, the general heath perception, F&V intake, choice of foods easy to chew and having a low or high BMI.

  6. European Vegetation Archive (EVA): an integrated database of European vegetation plots

    DEFF Research Database (Denmark)

    Chytrý, M; Hennekens, S M; Jiménez-Alfaro, B

    2015-01-01

    The European Vegetation Archive (EVA) is a centralized database of European vegetation plots developed by the IAVS Working Group European Vegetation Survey. It has been in development since 2012 and first made available for use in research projects in 2014. It stores copies of national and regional...... data source for large-scale analyses of European vegetation diversity both for fundamental research and nature conservation applications. Updated information on EVA is available online at http://euroveg.org/eva-database....

  7. National And European Law: Problem Of Implementation

    Directory of Open Access Journals (Sweden)

    Olga M. Mesheriakova

    2014-09-01

    Full Text Available Present article is devoted to one of the main problems for all integration communities –problem of implementation of the integration law norms in the national legal system of member states. Author, on the example of certain member states of the European Union considers mechanism of the European Union law action in it's member states. In the article constitutional norms of the number of member states which set a ratio of the national and European laws are analyzed. According to the principle of competence giving, member states voluntary transfer part of the competence to the European Union. Competences which are transferred to the member states are the competences of the European Union. In the course of research author investigates opinions of scientists and analyze regulations. Author pays separate attention to the question of the European Union creation. For example, author notes that consideration by the Federal Constitutional Court in Karlsruhe of the question of compliance of the Treaty to the Constitution of Germany became neither more nor less a most important milestone on the way of Treaty on the European Union ratification by Germany. In the decision on this matter Court defined a number of reference points for the purpose of possible excise if the European integration elimination out of those limits which are set for the government of Germany by its Constitution. The decision of the Constitutional Court of Germany though meant approval of the Treaty on the European Union in the political sense, it was nevertheless unambiguously directed against broad interpretation and federalist vision of the European integration. It is obvious that states, on the basis of constitutional norms may leave contracts that are burdensome for them. It concerns not only international treaties, but also Treaties of the European Union.

  8. The european hematology association roadmap for european hematology research : A consensus document

    NARCIS (Netherlands)

    A. Engert (Andreas); C.L. Balduini (Carlo); A. Brand (Anneke); B. Coiffier (Bertrand); C. Cordonnier (Charlotte); H. Döhner (Hartmut); De Wit, T.D. (Thom Duyvené); Eichinger, S. (Sabine); W.E. Fibbe (Willem); Green, T. (Tony); De Haas, F. (Fleur); A. Iolascon (Achille); T. Jaffredo (Thierry); F. Rodeghiero (Francesco); G. Salles (Gilles); J.J. Schuringa (Jan Jacob)

    2016-01-01

    textabstractThe European Hematology Association (EHA) Roadmap for European Hematology Research highlights major achievements in diagnosis and treatment of blood disorders and identifies the greatest unmet clinical and scientific needs in those areas to enable better funded, more focused European

  9. EERA and Its European Conferences on Educational Research: A Patchwork of Research on European Educational Research

    Science.gov (United States)

    Keiner, Edwin; Hofbauer, Susann

    2014-01-01

    The process of Europeanisation is closely linked to the process of an emerging European Educational Research Area and an education research identity. The European Conferences on Educational Research (ECER), European Educational Research Association (EERA) and its networks are involved in new directions and strands of educational research in…

  10. A European legal method? On European private law and scientific method

    NARCIS (Netherlands)

    Hesselink, M.

    2009-01-01

    This article examines the relationship between European private law and scientific method. It argues that a European legal method is a good idea. Not primarily because it will make European private law scholarship look more scientific, but because a debate on the method of a normative science

  11. Metadata-catalogue of European spatial datasets

    NARCIS (Netherlands)

    Willemen, J.P.M.; Kooistra, L.

    2004-01-01

    In order to facilitate a more effective accessibility of European spatial datasets, an assessment was carried out by the GeoDesk of the WUR to identify and describe key datasets that will be relevant for research carried out within WUR and MNP. The outline of the Metadata catalogue European spatial

  12. The Political Psychology of European Integration

    DEFF Research Database (Denmark)

    Manners, Ian James

    2015-01-01

    express for the EU and fellow Europeans, and the passion with which supporters of the EU argue for greater integration and enlargement are today central to understanding European integration. Being mindful of Europe - reflecting on the ways in which psychology and politics are deeply implicated in under...

  13. European labour law legislation 2012/2013

    NARCIS (Netherlands)

    Peters, S.S.M.

    2012-01-01

    This publication brings together the most important European and international regulation in the area of labour law. It includes regulations from, among others, the European Union, the Council of Europe and the International Labour Organisation. The selected regulations are decisive to some extent

  14. Profile and competences for the European dentist.

    NARCIS (Netherlands)

    Plasschaert, A.J.M.; Holbrook, W.P.; Delap, E.; Martinez, C.; Walmsley, A.D.

    2005-01-01

    This paper presents the profile and competences for the European Dentist as approved by the General Assembly of the Association for Dental Education in Europe at its annual meeting held in Cardiff in September 2004. A task-force drafted the document, which was then sent to all European Dental

  15. European Industrial Doctorates: Marie Curie Actions

    Science.gov (United States)

    European Commission, 2012

    2012-01-01

    European industrial doctorates are joint doctoral training projects funded by the European Union (EU) and open to all research fields. The project brings together an academic participant (university, research institution, etc.) and a company. They have to be established in two different EU Member States or associated countries. Associated partners…

  16. European side markers effect on traffic safety

    NARCIS (Netherlands)

    Roelfsema, A.; Theeuwes, J.; Alferdinck, J.W.A.M

    1999-01-01

    In 1993 new European legislation regarding side-markers for passenger cars became effective. Volvo requested the TNO-Human Factors Research Institute (HFRI) to investigate the possible safety benefit of this European side-markers configuration. A test panel at TNO- HFRI was used to determine the

  17. Is there a European way of war?

    DEFF Research Database (Denmark)

    Vennesson, P.; Breuer, F.; de Franco, Chiara

    2009-01-01

    Europe is the region of the world where the network of security institutions is the densest. Yet, these institutions did not erase differences about conceptions of force employment among European countries and between European countries and the United States. Why have concepts of military power a...

  18. Sustainability Begets Unsustainability?: The European Union's Drive ...

    African Journals Online (AJOL)

    The mandatory target for the European Union (EU) for the use of renewable energy in transport by the year 2020 has created a situation where the African continent has witnessed a number of European agricultural firms gaining access to large tracts of land in order to grow crops for the production of agrofuels. This article ...

  19. Benchmarking European Gas Transmission System Operators

    DEFF Research Database (Denmark)

    Agrell, Per J.; Bogetoft, Peter; Trinkner, Urs

    This is the final report for the pan-European efficiency benchmarking of gas transmission system operations commissioned by the Netherlands Authority for Consumers and Markets (ACM), Den Haag, on behalf of the Council of European Energy Regulators (CEER) under the supervision of the authors....

  20. European Values Study 1981-2017

    NARCIS (Netherlands)

    Luijkx, A.R.C.M.; Halman, L.C.J.M.

    2017-01-01

    The European Values Study is a large-scale, cross-national, and longitudinal survey research program on basic human values providing insight into the ideas, beliefs, preferences, attitudes, values and opinions of citizens all over Europe. It is a unique research project on how Europeans think about

  1. ESMN / European Solar Physics Research Area

    NARCIS (Netherlands)

    Rutten, R.J.

    1999-01-01

    I briefly present the European Solar Magnetometry Network as a contemporary example of solar physics collaboration across European borders,and I place it in larger-scale context by discussing the past and future of Europe-wide solar physics organization.Solar physics from space is inherently

  2. Changing European Governance, Changing Research and Innovation

    DEFF Research Database (Denmark)

    Borrás, Susana

    This chapter examines two fundamental dimensions of the changing European governance, namely the coordination of national policies and the changes in membership (accession of Central and Eastern European Countries in the mid-2000, and of Brexit in late 2010s). In particular this chapter looks...

  3. The Future of Copyright Management: European Perspectives.

    Science.gov (United States)

    Battisti, Michele

    This paper presents European perspectives on the future of copyright management. The first section is an overview of intellectual property rights in Europe, including differences between copyright countries and "droit d'auteur" countries. The second section addresses European Community legal policy, including examples related to the…

  4. European Rabbits as Reservoir for Coxiella burnetii.

    Science.gov (United States)

    González-Barrio, David; Maio, Elisa; Vieira-Pinto, Madalena; Ruiz-Fons, Francisco

    2015-06-01

    We studied the role of European rabbits (Oryctolagus cuniculus) as a reservoir for Coxiella burnetii in the Iberian region. High individual and population seroprevalences observed in wild and farmed rabbits, evidence of systemic infections, and vaginal shedding support the reservoir role of the European rabbit for C. burnetii.

  5. European Frontiers in RF GaN

    NARCIS (Netherlands)

    Vliet, F.E. van; Hek, P. de

    2015-01-01

    In this overview paper, a summary of the European academic and industrial status on RF GaN is given. The roles of EDA and ESA are highlighted, and examples of on-going research activities are presented. A set of HPAs over frequency, representative of today's European status, is discussed. © 2015

  6. Learning Environment Perceptions of European University Students

    NARCIS (Netherlands)

    Kanselaar, G.; Wierstra, R.F.A.; Linden, J.L. van der; Lodewijks, H.G.L.C.

    1999-01-01

    This article describes a study of the experiences of 610 Dutch students and 241 European students who studied at least three months abroad within the framework of an international exchange program. The Dutch students went to a university in another European country and the foreign students went to a

  7. A European Vision for Adult Education

    Science.gov (United States)

    Waddington, Sue; Tuckett, Alan; Boucher, Fiona

    2012-01-01

    The National Institute of Adult Continuing Education (NIACE) is the UK national coordinator for the European Agenda for Adult Learning, with the challenge of creating a coherent message across the four countries to inform European cooperation on adult learning. To start the debate, the journal staff asked Sue Waddington, Alan Tuckett, and Fiona…

  8. Insights into the European Years’ Communication Toolboxes

    Directory of Open Access Journals (Sweden)

    Camelia-Mihaela Cmeciu

    2012-08-01

    Full Text Available Since 1983 the European syntagm “unity in diversity” has been implemented in the European Years’ communication campaigns. Dependent on subsidiarity and decentralization, European Years focus on a specific issue which constitutes the subject of a year-long awareness campaign. Beyond the involvement of Europe’s citizens through their local, regional and national authorities in the implementation of the European Years’ policies, there is a unity at the level of the visual communication of the EU by two important image-building elements: EY logos and communication toolboxes. The European Years’ communication toolboxes can be considered signs of inclusion since every organization is expected to customize the templates in the official campaign design of the European Year. The analysis will focus on the image-building elements of three European Years (2010, 2011, 2012. Having social semiotics as the qualitative research method and the analytical framework based on the distinction between design resources and representational resources, I will analyze the double layers of the high intensity point of inclusion: (1 the European Years’ branding process; (2 the visual deontic modality within the visual guidelines of the EY communication toolbox.

  9. European Sustainable Construction Regulation : Homogeneity and Attention

    NARCIS (Netherlands)

    Van der Heijden, J.; Van Bueren, E.M.

    2011-01-01

    Understanding the negative impact of the construction industry and the built environment on the ecological environment, the European Commission (EC) aims to harmonise and improve sustainable construction regulatory frameworks in Member States of the European Union. This paper discusses the topics of

  10. Training Issues for the European Automotive Industry

    NARCIS (Netherlands)

    Dankbaar, B.

    1996-01-01

    Provides an overview of the results of an investigation carried out for the European Commission. Aims to identify themes and issues in the field of continuing training in the European automotive industry. A large number of interviews were carried out in all the major car manufacturing countries of

  11. Political values in a European museum

    NARCIS (Netherlands)

    Molema, Marijn; Huistra, Pieter; Wirt, Daniel

    2014-01-01

    The last decades have seen the rise of a European politic of cultural identity. One of the most recent initiatives in this respect, dating from 2007, is the House of European History that is due to open its doors in 2015. In this article, we investigate the recent history of the House, in particular

  12. Transnational Lives in European Educational Research

    Science.gov (United States)

    Lawn, Martin

    2014-01-01

    Transnational collaboration by educational researchers in Europe has grown fast since the mid-1990s and the means to support it have become more easily accessible. A study of the growth of the European Educational Research Association (EERA) since its foundation in the mid-1990s shows how transnational research in European education began, and how…

  13. The European Insurance Industry: A PEST Analysis

    Directory of Open Access Journals (Sweden)

    Charmaine Barbara

    2017-05-01

    Full Text Available The insurance industry plays an important role for European economic stability and the threats and opportunities it faces should be carefully determined. In this paper we highlight the main challenges by using a Political, Economic, Social and Technological (PEST analysis. This work applies conventional actuarial thought on this area by focusing strictly on the European sector.

  14. Five political ideas of European contract law

    NARCIS (Netherlands)

    Hesselink, M.W.

    2011-01-01

    This paper explores the possible implications of leading contemporary theories of political philosophy for some of the main questions that the political institutions of the European Union will have to decide on concerning the future of European contract law. Thus, it explores what a utilitarian,

  15. The Politics of European Human Rights Culture

    Czech Academy of Sciences Publication Activity Database

    Agha, Petr

    2017-01-01

    Roč. 7, č. 3 (2017), s. 200-215 ISSN 1805-8396 R&D Projects: GA ČR GA13-30299S Institutional support: RVO:68378122 Keywords : Court of Justice of the European Union * European Court of Human Rights * religious symbols Subject RIV: AG - Legal Sciences

  16. Brexit and the problem of European disintegration

    DEFF Research Database (Denmark)

    Rosamond, Ben

    2016-01-01

    The Brexit referendum provokes speculation about the likelihood of European disintegration. This article discusses how scholarship might deal with the issue of disintegration and argues that it should be thought of as an indeterminate process rather than an identifiable outcome. Within the EU...... within which European integration has been embedded historically....

  17. ENERGY POLICY IN THE EUROPEAN UNION

    OpenAIRE

    Pazderníková, Kamila

    2006-01-01

    This work focuses on the reasons and beginnings of creation of European energy policy, the role of environment in this policy and different kinds of energy sources with special focus on the renewable ones. The hot topic of liberalization of European energy policy is also discussed. Powered by TCPDF (www.tcpdf.org)

  18. A comparative approach to European Union citizenship

    NARCIS (Netherlands)

    F.A.N.J. Goudappel (Flora)

    2004-01-01

    textabstractAfter a short description of the theoretical and philosophical background of the notions of citizenship and nationality, the contents of European Union citizenship will be described. This notion of European Citizenship will then be compared to the notions of citizenship and nationality

  19. Do European consumers use nutrition labels?

    DEFF Research Database (Denmark)

    Wills, Josephine M.; Grunert, Klaus G.; Celemín, Laura Fernández

    2009-01-01

    Nutrition labelling on food packages becomes more and more widespread in the European Union. Such information is not compulsory, unless a nutrition or health claim is made. However, how do consumers use nutrition information? Two European studies are currently assessing whether nutrition...... knowledge about nutrition and are able to use nutrition labels to identify healthier products within a category....

  20. Quality of Power Supply in European View

    Directory of Open Access Journals (Sweden)

    Zdenek Hradilek

    2008-01-01

    Full Text Available This paper deals with comparisons of situation in quality of power supply regulation in some of European countries, which are interested in CEER (Council of European Energy Regulators. Especially we focused our analysis on reliability indices, relationship between supplier and consumer and electric power supply aftereffects. There are discussed differences in each countries and comparison with situation in Czech Republic.