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Sample records for eucalyptus globulus plants

  1. Phytoremediation efficiency OF CD by Eucalyptus globulus transplanted from polluted and unpolluted sites.

    Science.gov (United States)

    Luo, Jie; Qi, Shihua; Peng, Li; Wang, Jinji

    2016-01-01

    The capacity of plants to uptake heavy metals from contaminated soils has shown great phytoremediation potential. The development, resistibility and Cd extraction of Eucalyptus globulus individuals from metalliferous and clean sites in different years were analyzed under a specific environment. Eucalyptus globulus planted in Guiyu for phytoremediation or cultivated in an uncontaminated, natural environment for economic purposes were transplanted to Yuecheng town, which, in recent years, has been involved in the e-waste dismantling and recycling business, to compare the phytoremediation efficiency of Eucalyptus globulus trees grown in different environments. Trees cultivated in polluted areas can remove far more Cd and Hg from the contaminated soil than the individuals from clean soils because metalliferous Eucalyptus globulus can produce more biomass and uptake more heavy metals than nonmetalliferous plants per year. As polluted environments negatively affect the growth of plants, we speculated that the phytoremediation efficiency of metalliferous Eucalyptus globulus should decrease over time and that nonmetalliferous trees should adapt to the local environment.

  2. A model system to study the lignification process in Eucalyptus globulus.

    Science.gov (United States)

    Araújo, Pedro; Cesarino, Igor; Mayer, Juliana Lischka Sampaio; Ferrari, Ilse Fernanda; Kiyota, Eduardo; Sawaya, Alexandra Christine Helena Frankland; Paes Leme, Adriana Franco; Mazzafera, Paulo

    2014-09-01

    Recalcitrance of plant biomass is closely related to the presence of the phenolic heteropolymer lignin in secondary cell walls, which has a negative effect on forage digestibility, biomass-to-biofuels conversion and chemical pulping. The genus Eucalyptus is the main source of wood for pulp and paper industry. However, when compared to model plants such as Arabidopsis thaliana and poplar, relatively little is known about lignin biosynthesis in Eucalyptus and only a few genes were functionally characterized. An efficient, fast and inexpensive in vitro system was developed to study lignification in Eucalyptus globulus and to evaluate the potential role of candidate genes in this biological process. Seedlings were grown in four different conditions, in the presence or absence of light and with or without sucrose in the growth medium, and several aspects of lignin metabolism were evaluated. Our results showed that light and, to a lesser extent, sucrose induced lignin biosynthesis, which was followed by changes in S/G ratio, lignin oligomers accumulation and gene expression. In addition, higher total peroxidase activity and differential isoperoxidase profile were observed when seedlings were grown in the presence of light and sucrose. Peptide sequencing allowed the identification of differentially expressed peroxidases, which can be considered potential candidate class III peroxidases involved in lignin polymerization in E. globulus. © 2014 Scandinavian Plant Physiology Society.

  3. Energy evaluation of the Eucalyptus globulus and the Eucalyptus nitens in the north of Spain (Cantabria)

    International Nuclear Information System (INIS)

    Perez, S.; Renedo, C.J.; Ortiz, A.; Manana, M.; Silio, D.

    2006-01-01

    This work studied the potential use of the waste from Eucalyptus globulus and Eucalyptus nitens as energy crops, evaluating young and adult stages of both in all four seasons of the year with different moisture contents. The study was carried out made in Cantabria (North coast of Spain), located at latitude 43 o 28'N, and longitude 3 o 48'W. In this region, 29,513ha are dedicated to the growth of Eucalyptus, with about 80% E. globulus, and 20% E. nitens. Six different plantations have been analyzed and their bioclimatic diagrams determined. After the collection of samples the potential energy of every sample was obtained, they were weighed, analyzed and burned, giving a mean net calorific value of 17,384 and 17,927kJ/kg in the adult stage of E. globulus and E. nitens, respectively. The results for the young stage of both species were 17,708 and 18,670kJ/kg. Moisture content in the samples has a great influence on power production. Finally, the economic and environmental consequences of these crop species for the region of Cantabria were analyzed. (author)

  4. Eucalyptus globulus extract protects upon acetaminophen-induced kidney damages in male rat

    Science.gov (United States)

    Dhibi, Sabah; Mbarki, Sakhria; Elfeki, Abdelfettah; Hfaiedh, Najla

    2014-01-01

    Plants have historically been used in treating many diseases. Eucalyptus globules, a rich source of bioactive compounds, and have been shown to possess antioxidative properties. The purpose of this study, carried out on male Wistar rats, was to evaluate the beneficial effects of Eucalyptus globulus extract upon acetaminophen-induced damages in kidney. Our study is realized in the Department of Biology, Faculty of Sciences of Sfax (Tunisia). 32 Wistar male rats; were divided into 4 batches: a control group (n=8), a group of rats treated with acetaminophen (goomg/kg) by intraperitoneal injection during 4 days (n=8), a group receiving Eucalyptus globulus extract (130 mg of dry leaves/kg/day) in drinking water during 42 days after 2 hours of acetaminophen administration (during 4 days) (n=8) and group received only Eucalyptus (n=8) during 42 days. After 6 weeks, animals from each group were rapidly sacrificed by decapitation. Blood serum was obtained by centrifugation. Under our experimental conditions, acetaminophen poisoning resulted in an oxidative stress evidenced by statistically significant losses in the activities of catalase (CAT), superoxide-dismutase (SOD), glutathione-peroxidase (GPX) activities and an increase in lipids peroxidation level in renal tissue of acetaminophen-treated group compared with the control group. Acetaminophen also caused kidney damage as evident by statistically significant (p<0.05) increase in levels of creatinine and urea and decreased levels of uric acid and proteins in blood. Histological analysis demonstrated alteration of proximal tubules, atrophy of the glomerule and dilatation of urinary space. Previous administration of plant extract is found to alleviate this acetaminophen-induced damage. PMID:24856382

  5. O comportamento de pés-mãe de Eucalyptus globulus Labill. sujeitos a diferentes níveis de azoto e potássio Influence of nitrogen and potassium on Eucalyptus globulus Labill. mother plants

    Directory of Open Access Journals (Sweden)

    H. M. Ribeiro

    2007-07-01

    crescimento dos pés-mãe de E. globulus e para o sucesso do enraizamento das suas estacas, devendo a fertilização azotada destas plantas ser sujeita a um cuidado trabalho de optimização.In Portugal the traditional propagation of Eucalyptus globulus Labill (E. globulus by seed is being replaced by vegetative propagation by stem cuttings. However, for E. globulus, there is no sufficient information related to the management of mother plant fertilization. So, it is important to study the relationship between the fertilization of the mother plant and the rooting ability of the cuttings. With the aim of evaluating the effect of nitrogen and potassium on E. globulus mother plants, a fertilization experiment was performed during 2 years. 2 clones of E. globulus (clone HD161 and clone CN5 were fertilized, periodically, with 6 different nutrient solutions corresponding to 3 levels of nitrogen (50, 100 and 200 mg N L-1 and 2 levels of potassium (50 and 100 mg K L-1. In 1998, stock plants were subjected to formative pruning. In 1999, biomass, number of cuttings produced by the mother plants and rooting ability of the cuttings were evaluated. With the two levels of potassium applied no significant effects were found. On the contrary, increasing levels of nitrogen applied to the mother plants led to: i an increase of total biomass, ii a higher production of stem cuttings, iii an improvement of the rooting ability of the cuttings. Results showed that nitrogen fertilization and, as consequence, nitrogen reserves, constitute a limiting factor for rooting in E. globulus cuttings. Thus, research on the optimization of nitrogen fertilization of E. globulus mother plants should be performed.

  6. Unravelling the bioherbicide potential of Eucalyptus globulus Labill: Biochemistry and effects of its aqueous extract

    Science.gov (United States)

    Reigosa, Manuel J.; Valentão, Patrícia; Andrade, Paula B.

    2018-01-01

    In the worldwide search for new strategies in sustainable weed management, the use of plant species able to produce and release phytotoxic compounds into the environment could be an effective alternative to synthetic herbicides. Eucalyptus globulus Labill. is known to be a source of biologically active compounds responsible for its phytotoxic and allelopathic properties. Our previous results demonstrated the bioherbicide potential of eucalyptus leaves incorporated into the soil as a green manure, probably through the release of phytotoxins into the soil solution. Thus, the aims of this study were to understand the phytotoxicity of the eucalyptus leaves aqueous extract applied in pre- and post-emergence, and to identify and quantify its potentially phytotoxic water-soluble compounds. The effects were tested on the germination and early growth of the model target species Lactuca sativa and Agrostis stolonifera, and on physiological parameters of L. sativa adult plants after watering or spraying application. Dose-response curves and ED50 and ED80 values for eucalyptus aqueous extracts revealed pre-emergence inhibitory effects on both target species, effects being comparable to the herbicide metolachlor. While spraying treatment reduced the aerial and root biomass and increased the dry weight/fresh weight ratio of lettuce adult plants, watering application reduced protein contents and chlorophyll concentrations with respect to control, reflecting different modes of action depending on the site of phytotoxin entry. Via HPLC analyses, a total of 8 phenolic compounds (chlorogenic, two ρ-coumaric derivatives, ellagic, hyperoside, rutin, quercitrin, and kaempferol 3-O-glucoside) and other 5 low weight organic acids (citric, malic, shikimic, succinic and fumaric acids) were obtained from aqueous extract, the latter being identified for the first time in E. globulus. Despite some phytotoxic effects were found on lettuce adult plants, the use of eucalyptus aqueous extract

  7. Unravelling the bioherbicide potential of Eucalyptus globulus Labill: Biochemistry and effects of its aqueous extract.

    Science.gov (United States)

    Puig, Carolina G; Reigosa, Manuel J; Valentão, Patrícia; Andrade, Paula B; Pedrol, Nuria

    2018-01-01

    In the worldwide search for new strategies in sustainable weed management, the use of plant species able to produce and release phytotoxic compounds into the environment could be an effective alternative to synthetic herbicides. Eucalyptus globulus Labill. is known to be a source of biologically active compounds responsible for its phytotoxic and allelopathic properties. Our previous results demonstrated the bioherbicide potential of eucalyptus leaves incorporated into the soil as a green manure, probably through the release of phytotoxins into the soil solution. Thus, the aims of this study were to understand the phytotoxicity of the eucalyptus leaves aqueous extract applied in pre- and post-emergence, and to identify and quantify its potentially phytotoxic water-soluble compounds. The effects were tested on the germination and early growth of the model target species Lactuca sativa and Agrostis stolonifera, and on physiological parameters of L. sativa adult plants after watering or spraying application. Dose-response curves and ED50 and ED80 values for eucalyptus aqueous extracts revealed pre-emergence inhibitory effects on both target species, effects being comparable to the herbicide metolachlor. While spraying treatment reduced the aerial and root biomass and increased the dry weight/fresh weight ratio of lettuce adult plants, watering application reduced protein contents and chlorophyll concentrations with respect to control, reflecting different modes of action depending on the site of phytotoxin entry. Via HPLC analyses, a total of 8 phenolic compounds (chlorogenic, two ρ-coumaric derivatives, ellagic, hyperoside, rutin, quercitrin, and kaempferol 3-O-glucoside) and other 5 low weight organic acids (citric, malic, shikimic, succinic and fumaric acids) were obtained from aqueous extract, the latter being identified for the first time in E. globulus. Despite some phytotoxic effects were found on lettuce adult plants, the use of eucalyptus aqueous extract

  8. Eucalyptus globulus extract protects against UVB-induced photoaging by enhancing collagen synthesis via regulation of TGF-β/Smad signals and attenuation of AP-1.

    Science.gov (United States)

    Park, Bom; Hwang, Eunson; Seo, Seul A; Cho, Jin-Gyeong; Yang, Jung-Eun; Yi, Tae-Hoo

    2018-01-01

    UV irradiation triggers the overproduction of matrix metalloproteinases and collagen degradation, which in turn causes increased pigmentation, dryness, and deep wrinkling of the skin. These chronic symptoms are collectively referred to as photoaging. Eucalyptus globulus is an evergreen tree that is widely used in cosmetics because of its antimicrobial activity. In this study, we investigated the protective effect of 50% ethanol extracts of Eucalyptus globulus on UV-induced photoaging in vitro and in vivo. Normal human dermal fibroblasts were treated with Eucalyptus globulus at concentrations ranging from 1 to 100 μg/mL after UVB or non-UVB irradiation. We found that Eucalyptus globulus suppressed the expression of MMPs and IL-6, but increased the expression of TGF-β1 and procollagen type 1. In addition, Eucalyptus globulus inhibited activation of the AP-1 transcription factor, an inducer of MMPs. Eucalyptus globulus was also found to regulate TGF-β/Smad signaling by reversing the activity of negative Smad regulators. Lastly, in vivo studies showed that topical application of Eucalyptus globulus on UVB-irradiated hairless mice reduced wrinkle formation and dryness by down-regulating MMP-1 and up-regulating expression of elastin, TGF-β1, and procollagen type 1. Taken together, these data suggest that Eucalyptus globulus may be a useful agent in cosmetic products. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. Nitrogen-Fixing Bacteria in Eucalyptus globulus Plantations

    Science.gov (United States)

    da Silva, Marliane de Cássia Soares; Paula, Thiago de Almeida; Moreira, Bruno Coutinho; Carolino, Manuela; Cruz, Cristina; Bazzolli, Denise Mara Soares; Silva, Cynthia Canedo; Kasuya, Maria Catarina Megumi

    2014-01-01

    Eucalypt cultivation is an important economic activity worldwide. In Portugal, Eucalyptus globulus plantations account for one-third of the total forested area. The nutritional requirements of this crop have been well studied, and nitrogen (N) is one of the most important elements required for vegetal growth. N dynamics in soils are influenced by microorganisms, such as diazotrophic bacteria (DB) that are responsible for biological nitrogen fixation (BNF), so the aim of this study was to evaluate and identity the main groups of DB in E. globulus plantations. Samples of soil and root systems were collected in winter and summer from three different Portuguese regions (Penafiel, Gavião and Odemira). We observed that DB communities were affected by season, N fertilization and moisture. Furthermore Bradyrhizobium and Burkholderia were the most prevalent genera in these three regions. This is the first study describing the dynamic of these bacteria in E. globulus plantations, and these data will likely contribute to a better understanding of the nutritional requirements of eucalypt cultivation and associated organic matter turnover. PMID:25340502

  10. Nitrogen-fixing bacteria in Eucalyptus globulus plantations.

    Science.gov (United States)

    da Silva, Marliane de Cássia Soares; Paula, Thiago de Almeida; Moreira, Bruno Coutinho; Carolino, Manuela; Cruz, Cristina; Bazzolli, Denise Mara Soares; Silva, Cynthia Canedo; Kasuya, Maria Catarina Megumi

    2014-01-01

    Eucalypt cultivation is an important economic activity worldwide. In Portugal, Eucalyptus globulus plantations account for one-third of the total forested area. The nutritional requirements of this crop have been well studied, and nitrogen (N) is one of the most important elements required for vegetal growth. N dynamics in soils are influenced by microorganisms, such as diazotrophic bacteria (DB) that are responsible for biological nitrogen fixation (BNF), so the aim of this study was to evaluate and identity the main groups of DB in E. globulus plantations. Samples of soil and root systems were collected in winter and summer from three different Portuguese regions (Penafiel, Gavião and Odemira). We observed that DB communities were affected by season, N fertilization and moisture. Furthermore Bradyrhizobium and Burkholderia were the most prevalent genera in these three regions. This is the first study describing the dynamic of these bacteria in E. globulus plantations, and these data will likely contribute to a better understanding of the nutritional requirements of eucalypt cultivation and associated organic matter turnover.

  11. Nitrogen-fixing bacteria in Eucalyptus globulus plantations.

    Directory of Open Access Journals (Sweden)

    Marliane de Cássia Soares da Silva

    Full Text Available Eucalypt cultivation is an important economic activity worldwide. In Portugal, Eucalyptus globulus plantations account for one-third of the total forested area. The nutritional requirements of this crop have been well studied, and nitrogen (N is one of the most important elements required for vegetal growth. N dynamics in soils are influenced by microorganisms, such as diazotrophic bacteria (DB that are responsible for biological nitrogen fixation (BNF, so the aim of this study was to evaluate and identity the main groups of DB in E. globulus plantations. Samples of soil and root systems were collected in winter and summer from three different Portuguese regions (Penafiel, Gavião and Odemira. We observed that DB communities were affected by season, N fertilization and moisture. Furthermore Bradyrhizobium and Burkholderia were the most prevalent genera in these three regions. This is the first study describing the dynamic of these bacteria in E. globulus plantations, and these data will likely contribute to a better understanding of the nutritional requirements of eucalypt cultivation and associated organic matter turnover.

  12. A model for predicting the growth of Eucalyptus globulus seedling stands in Bolivia

    Energy Technology Data Exchange (ETDEWEB)

    Guzman, G.; Morales, M.; Pukkala, T.; Miguel, S. de

    2012-11-01

    Eucalyptus globulus is one of the most planted species in the Inter-Andean Valleys of Bolivia, where growing conditions are different from most places where eucalyptus have been studied. This prevents a straightforward utilization of models fitted elsewhere. In this study a distance-independent individual-tree growth model for E. globulus plantations in Bolivia was developed based on data from 67 permanent sample plots. The model consists of sub-models for dominant height, tree diameter increment, height-diameter relationship and survival. According to model-based simulations, the mean annual increment with the optimal rotation length is about 13 m3 ha{sup -}1 yr{sup -}1 on medium-quality sites and 18 m{sup 3} ha{sup -}1 yr -1 on the best sites. A suitable rotation length for maximizing wood production is approximately 30 years on medium sites and 20 years on the most productive sites. The developed models provide valuable information for further studies on optimizing the management and evaluating alternative management regimes for the species. (Author) 22 refs.

  13. Acaricidal and repellent activities of essential oil of Eucalyptus globulus against Dermanyssus gallinae (Acari: Mesostigmata

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    Dehghani-Samani Amir

    2015-07-01

    Full Text Available Introduction: By considering an increase in drug resistance against red mites, finding the nonchemical herbal acaricide against Dermanyssus gallinae (De Geer (Acari: Mesostigmata is necessary to kill them and to reduce the chemical resistance against chemical acaricides in this specie. Dermanyssus gallinae is a potential vector of the causal agent of several viral diseases such as Equine encephalitis and St. Louis encephalitis. It can be a vector of bacteria such as Salmonella spp., Mycobacterium spp. and Erysipelothrix rhusiopathiae. It is also known to cause itching dermatosis in humans. In this study acaricidal and repellent activities of essential oil of Eucalyptus globulus against Dermanyssus gallinae were studied. Methods: After extracting the essential oil, different concentrations of the plant extract were prepared. Then, acaricidal effect of different concentrations was tested on poultry red mite, Dermanyssus gallinae, by dropping 3-4 drops of essential oil on mites. Repellent activity of essential oil was tested by Y-tube olfactometer bioassay. After the test, total number of killed and repellent mites reported. Results: Concentration of 1:2 or 50% had more acaricidal effect on mites. Also essential oil of Eucalyptus globulus had repellent activity against red mites. Conclusion: This study showed that essential oil of Eucalyptus globulus had acaricidal and repellent activities against red mites. Hence it might be used as a herbal acaricide against it to kill and to reduce the chemical resistance in this specie.

  14. Streptomyces rhizobacteria modulate the secondary metabolism of Eucalyptus plants.

    Science.gov (United States)

    Salla, Tamiris Daros; da Silva, Ramos; Astarita, Leandro Vieira; Santarém, Eliane Romanato

    2014-12-01

    The genus Eucalyptus comprises economically important species, such as Eucalyptus grandis and Eucalyptus globulus, used especially as a raw material in many industrial sectors. Species of Eucalyptus are very susceptible to pathogens, mainly fungi, which leads to mortality of plant cuttings in rooting phase. One alternative to promote plant health and development is the potential use of microorganisms that act as agents for biological control, such as plant growth-promoting rhizobacteria (PGPR). Rhizobacteria Streptomyces spp have been considered as PGPR. This study aimed at selecting strains of Streptomyces with ability to promote plant growth and modulate secondary metabolism of E. grandis and E. globulus in vitro plants. The experiments assessed the development of plants (root number and length), changes in key enzymes in plant defense (polyphenol oxidase and peroxidase) and induction of secondary compounds(total phenolic and quercetinic flavonoid fraction). The isolate Streptomyces PM9 showed highest production of indol-3-acetic acid and the best potential for root induction. Treatment of Eucalyptus roots with Streptomyces PM9 caused alterations in enzymes activities during the period of co-cultivation (1-15 days), as well as in the levels of phenolic compounds and flavonoids. Shoots also showed alteration in the secondary metabolism, suggesting induced systemic response. The ability of Streptomyces sp. PM9 on promoting root growth, through production of IAA, and possible role on modulation of secondary metabolism of Eucalyptus plants characterizes this isolate as PGPR and indicates its potential use as a biological control in forestry.

  15. Water relations link carbon and oxygen isotope discrimination to phloem sap sugar concentration in eucalyptus globulus

    International Nuclear Information System (INIS)

    Cernusak, L.A.; Farquhar, G.D.; Arthur, D.J; Pate, J.S.

    2002-01-01

    Full text: The carbon isotope ratio of phloem sap sugars has been previously observed to correlate strongly with the phloem sap sugar concentration in Eucalyptus globulus. We hypothesized that the correspondence between these two parameters results from co-linearity in their responses to variation in plant water potential. Carbon isotope discrimination is expected to decrease with decreasing plant water potential due to the influence of stomatal conductance on the ratio of intercellular to ambient CO 2 , concentrations (c 1 /c a ). Conversely, we expected the phloem sap sugar concentration to increase with decreasing plant water potential, thereby maintaining positive turgor pressure within the sieve tubes. The study comprised 40 individual Eucalyptus globulus trees growing in three plantations situated on opposing ends of a rainfall gradient in southwestern Australia. A strong correlation was observed between the carbon isotope ratio in phloem sap sugars and phloem sap sugar concentration. Carbon isotope discrimination correlated positively with shoot water potential, whereas phloem sap sugar concentration correlated negatively with shoot water potential. The relationship between carbon isotope discrimination measured in phloem sap sugars collected from the stem and c 1 /c a measured instantaneously on subtending leaves was close to that theoretically predicted. Accordingly, a strong, negative relationship was observed between instantaneous c 1 /c a and the phloem sap sugar concentration. Oxygen isotope discrimination in phloem sap sugars also correlated strongly with phloem sap sugar concentration. A theoretical model suggested that the observed variation in stomatal conductance was sufficient to account for the variation observed in oxygen isotope discrimination across the study. Results strongly support the contention that water relations form a mechanistic link between phloem sap sugar concentration and both instantaneous and integrated measures of the

  16. A NEW SPECIES OF INVASIVE GALL WASP (HYMENOPTERA: EULOPHIDAE: TETRASTICHINAE) ON BLUE GUM (EUCALYPTUS GLOBULUS) IN CALIFORNIA

    Science.gov (United States)

    The blue gum gall wasp, Selitrichodes globulus La Salle & Gates (Hymenoptera: Eulophidae: Tetrastichinae), is described as an invasive gall inducer on blue gum, Eucalyptus globulus (Myrtaceae), in California....

  17. Unravelling the bioherbicide potential of Eucalyptus globulus Labill: Biochemistry and effects of its aqueous extract.

    Directory of Open Access Journals (Sweden)

    Carolina G Puig

    Full Text Available In the worldwide search for new strategies in sustainable weed management, the use of plant species able to produce and release phytotoxic compounds into the environment could be an effective alternative to synthetic herbicides. Eucalyptus globulus Labill. is known to be a source of biologically active compounds responsible for its phytotoxic and allelopathic properties. Our previous results demonstrated the bioherbicide potential of eucalyptus leaves incorporated into the soil as a green manure, probably through the release of phytotoxins into the soil solution. Thus, the aims of this study were to understand the phytotoxicity of the eucalyptus leaves aqueous extract applied in pre- and post-emergence, and to identify and quantify its potentially phytotoxic water-soluble compounds. The effects were tested on the germination and early growth of the model target species Lactuca sativa and Agrostis stolonifera, and on physiological parameters of L. sativa adult plants after watering or spraying application. Dose-response curves and ED50 and ED80 values for eucalyptus aqueous extracts revealed pre-emergence inhibitory effects on both target species, effects being comparable to the herbicide metolachlor. While spraying treatment reduced the aerial and root biomass and increased the dry weight/fresh weight ratio of lettuce adult plants, watering application reduced protein contents and chlorophyll concentrations with respect to control, reflecting different modes of action depending on the site of phytotoxin entry. Via HPLC analyses, a total of 8 phenolic compounds (chlorogenic, two ρ-coumaric derivatives, ellagic, hyperoside, rutin, quercitrin, and kaempferol 3-O-glucoside and other 5 low weight organic acids (citric, malic, shikimic, succinic and fumaric acids were obtained from aqueous extract, the latter being identified for the first time in E. globulus. Despite some phytotoxic effects were found on lettuce adult plants, the use of eucalyptus

  18. Assessing nitrogen fixation in mixed- and single-species plantations of Eucalyptus globulus and Acacia mearnsii.

    Science.gov (United States)

    Forrester, David I; Schortemeyer, Marcus; Stock, William D; Bauhus, Jürgen; Khanna, Partap K; Cowie, Annette L

    2007-09-01

    Mixtures of Eucalyptus globulus Labill. and Acacia mearnsii de Wildeman are twice as productive as E. globulus monocultures growing on the same site in East Gippsland, Victoria, Australia, possibly because of increased nitrogen (N) availability owing to N(2) fixation by A. mearnsii. To investigate whether N(2) fixation by A. mearnsii could account for the mixed-species growth responses, we assessed N(2) fixation by the accretion method and the (15)N natural abundance method. Nitrogen gained by E. globulus and A. mearnsii mixtures and monocultures was calculated by the accretion method with plant and soil samples collected 10 years after plantation establishment. Nitrogen in biomass and soil confirmed that A. mearnsii influenced N dynamics. Assuming that the differences in soil, forest floor litter and biomass N of plots containing A. mearnsii compared with E. globulus monocultures were due to N(2) fixation, the 10-year annual mean rates of N(2) fixation were 38 and 86 kg ha(-1) year(-1) in 1:1 mixtures and A. mearnsii monocultures, respectively. Nitrogen fixation by A. mearnsii could not be quantified on the basis of the natural abundance of (15)N because such factors as mycorrhization type and fractionation of N isotopes during N cycling within the plant confounded the effect of the N source on the N isotopic signature of plants. This study shows that A. mearnsii fixed significant quantities of N(2) when mixed with E. globulus. A decline in delta(15)N values of E. globulus and A. mearnsii with time, from 2 to 10 years, is further evidence that N(2) was fixed and cycled through the stands. The increased aboveground biomass production of E. globulus trees in mixtures when compared with monocultures can be attributed to increases in N availability.

  19. Development of an acetic acid tolerant Spathaspora passalidarum strain through evolutionary engineering with resistance to inhibitors compounds of autohydrolysate of Eucalyptus globulus

    DEFF Research Database (Denmark)

    Morales, Paulina; Gentina, Juan Carlos; Aroca, German

    2017-01-01

    -fold higher than those obtained with the native strain, respectively. Inhibitors composition present inEucalyptus globulus autohydrolysate were (g L−1): acetic acid, 4.7; furfural, 1.0; HMF, 0.36; formic acid, 0.6;syringaldehyde, 0.14 and vanillin, 0.017. When Eucalyptus globulus autohydrolysate...

  20. Managing for water-use efficient wood production in Eucalyptus globulus plantations

    Science.gov (United States)

    Donald A. White; John F. McGrath; Michael G. Ryan; Michael Battaglia; Daniel S. Mendham; Joe Kinal; Geoffrey M. Downes; D. Stuart Crombie; Mark E. Hunt

    2014-01-01

    This paper tests the hypothesis that thinning and nitrogen fertiliser can increase the mass of wood produced per volume of water used (evapotranspiration) by plantations of Eucalyptus globulus. We have called this plantation water productivity (PWPWOOD) and argue that, for a given genotype, this term integrates the effects of management, site and climate on both...

  1. Are post-dispersed seeds of Eucalyptus globulus predated in the introduced range? Evidence from an experiment in Portugal

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    E. Deus

    2018-04-01

    Full Text Available Plantations of Eucalyptus globulus Labill. have been expanding rapidly worldwide. The species is considered invasive in several regions. While in the native range, post-dispersal seed predation is known to severely limit eucalypt recruitment, there is no experimental evidence of seed predation in the introduced range. We hypothesised that E. globulus seeds largely escape predation in Portugal, which may explain its prolific recruitment in some locations. We tested this hypothesis in central Portugal by exposing E. globulus seeds to the local fauna. For comparison purposes, we also used seeds from locally common species: Acacia dealbata Link (alien, larger, elaiosome-bearing seeds and Cistus salviifolius L. (native, similarly sized seeds. We installed 30 feeding stations across three study sites, each one dominated by one study species. Each feeding station featured four feeders with different animal-access treatments: invertebrates; vertebrates; full access; no access (control. We placed five seeds of each plant species every day in each feeder and registered the number of seeds missing, eaten and elaiosome detached over 9 summer days. Eucalyptus globulus seeds were highly attractive to fauna in the three sites. Nearly half of E. globulus seeds were predated or removed, thus contradicting our hypothesis. Surprisingly, E. globulus and A. dealbata seeds were used by animals in similar proportions and C. salviifolius seeds were the least preferred. Vertebrates were the predominant seed predators and preferred the alien seeds. Invertebrates used all seed species in similar proportions. We found spatial variation regarding the predominant type of seed predators and the levels of seed predation according to the following patterns: predominance of vertebrates; predominance of invertebrates; negligible seed predator activity. Locations with negligible seed predation were abundant and scattered across the study area. Such spatial variation may

  2. Mycosphaerella and Teratosphaeria species associated with Mycosphaerella Leaf Disease on Eucalyptus globulus in Portugal

    Energy Technology Data Exchange (ETDEWEB)

    Silva, M. C.; Machado, H. N.; Neves, L.; Araujo, C.; Phillips, J. L.

    2012-11-01

    Plantations of Eucalyptus globulus represent the main source of wood for the pulp and paper industry in Portugal and are affected by the complex of Mycosphaerella and Teratosphaeria species (Mycosphaerella leaf disease), which is an important foliage disease worldwide. This disease affect mainly young trees with juvenile-phase foliage, causing premature defoliation, decreased growth and wood production. Species of Mycosphaerella sensu lato reported on eucalypts in Portugal are M. communis, M. heimii, M. lateralis, M. madeirae, M. marksii M. walkeri, T. africana, T. molleriana, T. nubilosa and T. parva. In order to complete the survey, symptomatic leaves were collected from Eucalyptus globulus plantations. Morphological and molecular characterization was used to give an indication of the species occurrence and most frequent species (T. nubilosa) and the composition of the MLD complex that did not change after the latest review. (Author) 28 refs.

  3. Concerted transcription of auxin and carbohydrate homeostasis-related genes underlies improved adventitious rooting of microcuttings derived from far-red treated Eucalyptus globulus Labill mother plants.

    Science.gov (United States)

    Ruedell, Carolina Michels; de Almeida, Márcia Rodrigues; Fett-Neto, Arthur Germano

    2015-12-01

    Economically important plant species, such as Eucalyptus globulus, are often rooting recalcitrant. We have previously shown that far-red light enrichment applied to E. globulus donor-plants improved microcutting rooting competence and increased rooting zone/shoot carbohydrate ratio. To better understand this developmental response, the relative expression profiles of genes involved in auxin signaling (ARF6, ARF8, AGO1), biosynthesis (YUC3) and transport (AUX1, PIN1, PIN2); sucrose cleavage (SUS1, CWINV1), transport (SUC5), hexose phosphorylation (HXK1, FLN1) and starch biosynthesis (SS3) were quantified during adventitious rooting of E. globulus microcuttings derived from donor plants exposed to far-red or white light. Expression of auxin transport-related genes increased in the first days of root induction. Far-red enrichment of donor plants induced ARF6, ARF8 and AGO1 in microcuttings. The first two gene products could activate GH3 and other rooting related genes, whereas AGO1 deregulation of the repressor ARF17 may relief rooting inhibition. Increased sink strength at the basal stem with sucrose unloading in root tissue mediated by SUC and subsequent hydrolysis by SUS1 were also supported by gene expression profile. Fructose phosphorylation and starch biosynthesis could also contribute to proper carbon allocation at the site of rooting, as evidenced by increased expression of related genes. These data are in good agreement with increased contents of hexoses and starch at the cutting base severed from far-red exposed donor plants. To sum up, pathways integrating auxin and carbohydrate metabolism were activated in microcuttings derived from donor plants exposed to far red light enrichment, thereby improving rooting response in E. globulus. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  4. Dust loadings on some common plants near Lucknow city. [Acacia melanoxylon, Bauhinia malabarica, Bougain-villea glabra, Calotropis procera, Catharanthus roseus, Eucalyptus globulus, Ipomoea fistulosa and Peltophorum pterocarpum

    Energy Technology Data Exchange (ETDEWEB)

    Yunus, M.; Dwivedi, A.K.; Kulshreshtha, K.; Ahmad, K.J.

    1985-01-01

    Eight plant species - Acacia melanoxylon, Bauhinia malabarica, Bougain-villea glabra, Calotropis procera, Catharanthus roseus, Eucalyptus globulus, Ipomoea fistulosa and Peltophorum pterocarpum - were collected from a newly established suburb colony of Lucknow city, where the major pollutant is dust, to study the dust cleansing efficiency of the plant canopy and also to establish the correlation between the leaf morphological characteristics and their dust trapping potential. The dust load, in milligrams per square centimeter of leaf surface, was measured and related to foliar epidermal and cuticular characteristics, and morphological features.

  5. Eucalyptus

    Science.gov (United States)

    Blue Gum, Blue Mallee, Blue Mallee Oil, Eucalipto, Eucalypti Folium, Eucalyptol, Eucalyptol Oil, Eucalyptus blatter, Eucalyptus bicostata, Eucalyptus Essential Oil, Eucalyptus Oil, Eucalyptus fructicetorum, Eucalyptus globulus, Eucalyptus Leaf, Eucalyptus odorata, Eucalyptus ...

  6. Uso de antraquinona en cocción kraft de Eucalyptus globulus y Eucalyptus nitens

    OpenAIRE

    Moreira, Jorge Felipe Quintana

    2011-01-01

    En la presente disertación se verificó de manera experimental el efecto de la adición de Antraquinona al proceso de cocción batch convencional para una mezcla industrial de Eucalyptus globulus E. nitens.; en lo que respecta a su capacidad de aumentar la velocidad de la reacción de deslignificación y proporcionar estabilidad a las hemicelulosas y celulosas de la fibra. Lo anterior se logró con experiencias de laboratorio fijando las condiciones de cocción de acuerdo a la operación de los dig...

  7. Events Associated with Early Age-Related Decline in Adventitious Rooting Competence of Eucalyptus globulus Labill.

    Science.gov (United States)

    Aumond, Márcio L; de Araujo, Artur T; de Oliveira Junkes, Camila F; de Almeida, Márcia R; Matsuura, Hélio N; de Costa, Fernanda; Fett-Neto, Arthur G

    2017-01-01

    The development of adventitious roots is affected by several factors, including the age of the cutting donor plant, which negatively affects rooting capacity. Eucalyptus globulus quickly loses rooting capacity of cuttings as the donor plant ages, although the molecular and biochemical mechanisms behind this process are still unclear. To better understand the bases of rooting competence loss in E. globulus , the time required for a significant decline in rhizogenic ability without exogenous auxin was determined in microcuttings derived from donor plants of different ages after sowing. Tip cuttings of donor plants were severed before and after loss of rooting competence of microcuttings to test the hypothesis that auxin and carbohydrate homeostasis regulate rooting competence decline. There were no significant changes in concentration of carbohydrates, flavonoids, or proteins before and after the loss of rooting capacity. Peroxidase (EC 1.11.1.7) total activity increased with loss of rooting competence. Auxin concentration showed the opposite pattern. In good agreement, TAA1 , a key gene in auxin biosynthesis, had lower expression after loss of rooting capacity. The same applied to the auxin receptor gene TIR1 , suggesting reduced auxin sensitivity. On the other hand, genes associated with auxin response repression ( TPL , IAA12 ) or with the action of cytokinins, the rhizogenesis inhibitor-related ARR1 , showed higher expression in plants with lower rooting competence. Taken together, data suggest that age negatively affects E. globulus rooting by a combination of factors. Decreased endogenous auxin concentration, possibly caused by less biosynthesis, lower auxin sensitivity, higher expression of genes inhibiting auxin action, as well as of genes related to the action of cytokinins, appear to play roles in this process.

  8. Events Associated with Early Age-Related Decline in Adventitious Rooting Competence of Eucalyptus globulus Labill

    Science.gov (United States)

    Aumond, Márcio L.; de Araujo, Artur T.; de Oliveira Junkes, Camila F.; de Almeida, Márcia R.; Matsuura, Hélio N.; de Costa, Fernanda; Fett-Neto, Arthur G.

    2017-01-01

    The development of adventitious roots is affected by several factors, including the age of the cutting donor plant, which negatively affects rooting capacity. Eucalyptus globulus quickly loses rooting capacity of cuttings as the donor plant ages, although the molecular and biochemical mechanisms behind this process are still unclear. To better understand the bases of rooting competence loss in E. globulus, the time required for a significant decline in rhizogenic ability without exogenous auxin was determined in microcuttings derived from donor plants of different ages after sowing. Tip cuttings of donor plants were severed before and after loss of rooting competence of microcuttings to test the hypothesis that auxin and carbohydrate homeostasis regulate rooting competence decline. There were no significant changes in concentration of carbohydrates, flavonoids, or proteins before and after the loss of rooting capacity. Peroxidase (EC 1.11.1.7) total activity increased with loss of rooting competence. Auxin concentration showed the opposite pattern. In good agreement, TAA1, a key gene in auxin biosynthesis, had lower expression after loss of rooting capacity. The same applied to the auxin receptor gene TIR1, suggesting reduced auxin sensitivity. On the other hand, genes associated with auxin response repression (TPL, IAA12) or with the action of cytokinins, the rhizogenesis inhibitor-related ARR1, showed higher expression in plants with lower rooting competence. Taken together, data suggest that age negatively affects E. globulus rooting by a combination of factors. Decreased endogenous auxin concentration, possibly caused by less biosynthesis, lower auxin sensitivity, higher expression of genes inhibiting auxin action, as well as of genes related to the action of cytokinins, appear to play roles in this process. PMID:29067033

  9. Characterization of abiotic stress genes from different species of eucalyptus

    International Nuclear Information System (INIS)

    Naz, S.; Kausar, H.; Saleem, F.; Zafarullah, A.

    2015-01-01

    The stresses causing dehydration damage to the plant cell like cold, drought, and high salinity are the most frequent environmental stresses that influence plant growth, development and restraining productivity in cultivated areas world-wide. Many drought, salinity and cold inducible genes causing tolerance to environmental stresses in many plants include Dehydrin1 (DHN1), Dehydrin2 (DHN2), Dehydrin10 (DHN10), putative phosphate transporter (Ecpt2), choline monooxygenase (CMO) and DREB/CBF1c genes. Gene specific primer pairs were designed for each gene using DNAStar software. These genes were amplified from different species of eucalyptus such as Eucalyptus camaldulensis, E. globulus, E. tereticornis and E. gunii through PCR. Dehydrin2 gene of E. camaldulensis and dehydrin10 gene of E. globulus were cloned using the TA Cloning Kit with pCR 2.1 vector and sequenced. The Dehydrin genes sequences were submitted to GeneBank: Eucalyptus globulus dehydrin10 gene (Accession No. HG915712) and E. camaldulensis dehydrin 2 gene (Accession No. HG813113). The amino acid sequence of Dehydrin10 from E. globulus showed 97% homology to E. globulus DHN10 (JN052210) and Dehydrin2 from E. camaldulensis presented 94% homology to E. globulus DHN2 (JN052209). These genes can be employed in generating drought resistant crop plants. (author)

  10. Mycosphaerella and Teratosphaeria species associated with leaf diseases on Eucalyptus globulus in southern Brazil

    NARCIS (Netherlands)

    Teodoro, M.G.; Ferreira, M.A.; Guimarães, L.M.S.; Mafia, R.G.; Groenewald, J.Z.; Crous, P.W.; Alfenas, A.C.

    2012-01-01

    Leaf blight and defoliation caused by Teratosphaeria species is one of the most important leaf diseases of Eucalyptus globulus. Due to the importance of this tree species for the production of pulp and paper, and recent reports of severe leaf disease symptoms in Brazil, the present study was

  11. Comparison of Fractionation Techniques of CO2 Extracts from Eucalyptus Globulus - Composition and Insecticidal Activity

    Czech Academy of Sciences Publication Activity Database

    Topiař, Martin; Sajfrtová, Marie; Pavela, R.; Machalová, Zdeňka

    2015-01-01

    Roč. 97, FEB 2015 (2015), s. 202-210 ISSN 0896-8446 R&D Projects: GA TA ČR TA01010578 Institutional support: RVO:67985858 Keywords : supercritical fractinacion * eucalyptus globulus * insecticidal activity Subject RIV: CI - Industrial Chemistry , Chemical Engineering Impact factor: 2.579, year: 2015

  12. Macrocarpal C isolated from Eucalyptus globulus inhibits dipeptidyl peptidase 4 in an aggregated form.

    Science.gov (United States)

    Kato, Eisuke; Kawakami, Kazuhiro; Kawabata, Jun

    2018-12-01

    Dipeptidyl peptidase 4 (DPP-4) inhibitors are used for the treatment of type-2 diabetes mellitus. Various synthetic inhibitors have been developed to date, and plants containing natural DPP-4 inhibitors have also been identified. Here, 13 plant samples were tested for their DPP-4 inhibitory activity. Macrocarpals A-C were isolated from Eucalyptus globulus through activity-guided fractionation and shown to be DPP-4 inhibitors. Of these, macrocarpal C showed the highest inhibitory activity, demonstrating an inhibition curve characterised by a pronounced increase in activity within a narrow concentration range. Evaluation of macrocarpal C solution by turbidity, nuclear magnetic resonance spectroscopy and mass spectrometry indicated its aggregation, which may explain the characteristics of the inhibition curve. These findings will be valuable for further study of potential small molecule DPP-4 inhibitors.

  13. Control of Botrytis cinerea in Eucalyptus globulus Mini-Cuttings Using Clonostachys and Trichoderma Strains Control de Botrytis cinerea en miniestacas de Eucalyptus globulus Utilizando Cepas de Clonostachys y Trichoderma

    Directory of Open Access Journals (Sweden)

    Salomé Zaldúa

    2010-12-01

    Full Text Available Botrytis cinerea Pers. ex Fr. causes the disease known as gray mold in more than 200 hosts. It is one of the most important pathogens in Chilean forest nurseries and Eucalyptus globulus Labill. is one of the most susceptible species, especially in vegetative reproduction systems. Clonostachys and Trichoderma strains were selected as potential biocontrol agents of gray mold in previous research by the authors. The objective of this study was to evaluate the effectiveness of antagonistic fungi to control B. cinerea in E. globulus mini-cuttings. Five fungi strains were tested and applied weekly, two Clonostachys and three Trichoderma (5 x 10(6 conidia mL-1. In addition, comparison treatments were also used: absolute control (water and fungicide application. The experiment was carried out under operational conditions to produce E. globulus mini-cuttings. The Clonostachys UDC-A10 and UDC-A11 strains reduce mini-cutting mortality caused by B. cinerea in 54 and 71%, respectively, and with effects similar to those achieved by fungicides. Clonostachys UDC-A11 reduces the disease progression rate with the same statistical results as fungicides. A negative effect of applying fungicides on rooting of the surviving mini-cuttings was also confirmed. These results demonstrate the effectiveness of Clonostachys as a control agent against gray mold disease in E. globulus mini-cuttings.Botrytis cinerea Pers. ex Fr. ocasiona la enfermedad conocida como moho gris en más de 200 hospederos. En Chile es uno de los patógenos más importantes en viveros forestales, siendo Eucalyptus globulus Labill. una de las especies más susceptibles, especialmente en los sistemas de reproducción vegetativa. En investigaciones previas, realizadas por los autores, se seleccionaron cepas de Clonostachys y Trichoderma como potenciales agentes de biocontrol del moho gris. El objetivo fue evaluar la eficacia de hongos antagonistas en el control de B. cinerea en mini-estacas de E

  14. Adaptación de la madera de Eucalyptus globulus a la normativa europea de durabilidad natural

    OpenAIRE

    Lorenzo, D.; Troya, M. T. de,; Baso, Carlos; Touza, M.; Prieto, M.J.

    2007-01-01

    La madera de Eucalyptus globulus en algunos países como Australia es recomendada para situaciones de riesgo de ataque de organismos xilófagos por su elevada durabilidad. En Galicia esta madera ha sido utilizada desde hace muchas décadas en estructuras y carpinterías, que aún hoy perduran. Sin embargo, la norma europea EN-350-2 encuadra la especie E. globulus en la peor categoría de durabilidad natural, situación que no se corresponde con la realidad. El objetivo de este proyecto es constatar ...

  15. Can Acceptable Pulp be Obtained from Eucalyptus globulus Wood Chips after Hemicellulose Extraction?

    OpenAIRE

    Francisco López; M. Trinidad García; Vicente Mena; J. Mauricio Loaiza; Minerva A. M. Zamudio; Juan C. García

    2014-01-01

    This study investigates the operating conditions used in the soda-anthraquinone pulping of Eucalyptus globulus wood after autohydrolysis pretreatment on the yield, kappa number, and brightness of the resulting unbleached pulp. Moreover, strength-related properties of the resulting handsheets was examined to identify the best pulping conditions and compare the outcome with that of a conventional soda-anthraquinone pulping process. The paper strength properties of the pulp were similar to or be...

  16. Physiological and biochemical responses to severe drought stress of nine Eucalyptus globulus clones: a multivariate approach.

    Science.gov (United States)

    Granda, Víctor; Delatorre, Carolina; Cuesta, Candela; Centeno, María L; Fernández, Belén; Rodríguez, Ana; Feito, Isabel

    2014-07-01

    Seasonal drought, typical of temperate and Mediterranean environments, creates problems in establishing plantations and affects development and yield, and it has been widely studied in numerous species. Forestry fast-growing species such as Eucalyptus spp. are an important resource in such environments, selected clones being generally used for production purposes in plantations in these areas. However, use of mono-specific plantations increases risk of plant loss due to abiotic stresses, making it essential to understand differences in an individual clone's physiological responses to drought stress. In order to study clonal differences in drought responses, nine Eucalyptus globulus (Labill.) clones (C14, C46, C97, C120, C222, C371, C405, C491 and C601) were gradually subjected to severe drought stress (<14% of field capacity). A total of 31 parameters, physiological (e.g., photosynthesis, gas exchange), biochemical (e.g., chlorophyll content) and hormonal (abscisic acid [ABA] content), were analysed by classic and multivariate techniques. Relationships between parameters were established, allowing related measurements to be grouped into functional units (pigment, growth, water and ABA). Differences in these units showed that there were two distinct groups of E. globulus clones on the basis of their different strategies when faced with drought stress. The C14 group (C14, C120, C405, C491 and C601) clones behave as water savers, maintaining high water content and showing high stomatal adjustment, and reducing their aerial growth to a great extent. The C46 group (C46, C97, C222 and C371) clones behave as water spenders, reducing their water content drastically and presenting osmotic adjustment. The latter maintains the highest growth rate under the conditions tested. The method presented here can be used to identify appropriate E. globulus clones for drought environments, facilitating the selection of material for production and repopulation environments. © The

  17. Effects of essential oils from Eucalyptus globulus leaves on soil organisms involved in leaf degradation.

    Directory of Open Access Journals (Sweden)

    Carla Martins

    Full Text Available The replacement of native Portuguese forests by Eucalyptus globulus is often associated with deleterious effects on terrestrial and aquatic communities. Several studies have suggested that such a phenomenon is linked with the leaf essential oils released into the environment during the Eucalyptus leaf degradation process. However, to date, the way these compounds affect leaf degradation in terrestrial systems i.e. by direct toxic effects to soil invertebrates or indirectly by affecting food of soil fauna, is still unknown. In order to explore this question, the effect of essential oils extracted from E. globulus leaves on terrestrial systems was investigated. Fungal growth tests with species known as leaf colonizers (Mucor hiemalis, Alternaria alternata, Penicillium sp., Penicillium glabrum and Fusarium roseum were performed to evaluate the antifungal effect of essential oils. In addition, a reproduction test with the collembolans Folsomia candida was done using a gradient of eucalyptus essential oils in artificial soil. The influence of essential oils on feeding behaviour of F. candida and the isopods Porcellio dilatatus was also investigated through food avoidance and consumption tests. Eucalyptus essential oils were lethal at concentrations between 2.5-20 µL/mL and inhibited growth of all fungal species between 1.25-5 µL/mL. The collembolan reproduction EC50 value was 35.0 (28.6-41.2 mg/kg and both collembola and isopods preferred leaves without oils. Results suggested that the effect of essential oils in leaf processing is related to direct toxic effects on fungi and soil fauna and to indirect effects on the quality and availability of food to soil invertebrates.

  18. The Eucalyptus terpene synthase gene family.

    Science.gov (United States)

    Külheim, Carsten; Padovan, Amanda; Hefer, Charles; Krause, Sandra T; Köllner, Tobias G; Myburg, Alexander A; Degenhardt, Jörg; Foley, William J

    2015-06-11

    Terpenoids are abundant in the foliage of Eucalyptus, providing the characteristic smell as well as being valuable economically and influencing ecological interactions. Quantitative and qualitative inter- and intra- specific variation of terpenes is common in eucalypts. The genome sequences of Eucalyptus grandis and E. globulus were mined for terpene synthase genes (TPS) and compared to other plant species. We investigated the relative expression of TPS in seven plant tissues and functionally characterized five TPS genes from E. grandis. Compared to other sequenced plant genomes, Eucalyptus grandis has the largest number of putative functional TPS genes of any sequenced plant. We discovered 113 and 106 putative functional TPS genes in E. grandis and E. globulus, respectively. All but one TPS from E. grandis were expressed in at least one of seven plant tissues examined. Genomic clusters of up to 20 genes were identified. Many TPS are expressed in tissues other than leaves which invites a re-evaluation of the function of terpenes in Eucalyptus. Our data indicate that terpenes in Eucalyptus may play a wider role in biotic and abiotic interactions than previously thought. Tissue specific expression is common and the possibility of stress induction needs further investigation. Phylogenetic comparison of the two investigated Eucalyptus species gives insight about recent evolution of different clades within the TPS gene family. While the majority of TPS genes occur in orthologous pairs some clades show evidence of recent gene duplication, as well as loss of function.

  19. The Role of Eucalyptus Globulus Forest and Products in Carbon Sequestration

    International Nuclear Information System (INIS)

    Arroja, L.; Dias, A.C.; Capela, I.

    2006-01-01

    This study is a contribution to the ongoing debate about the selection of the approach for carbon accounting in wood products to be used, in the future, in the national greenhouse gas inventories under the UNFCCC (United Nations Framework Convention on Climate Change). Two accounting approaches are used in this analysis: the stock-change approach and the atmospheric-flow approach. They are applied to the Portuguese Eucalyptus globulus forest sector. To achieve this objective, the fluxes of wood removed from the forest are tracked through its life cycle, which includes products manufacture (mainly pulp and paper), use and final disposal (landfilling, incineration and composting). This study develops a framework to the estimation of carbon sequestration in the forest of E. globulus, a fast growing species, more specifically, in the calculation of the conversion factors such as bark and foliage percentages and densities, used to convert wood volumes into total biomass. A mass balance approach based on real data from mills is also proposed, in order to assess carbon emissions from wood processing. The results show that E. globulus forest sector was a carbon sink, but the magnitude of the carbon sequestration differs substantially depending on the accounting approach used. The contribution of the forest ecosystem was smaller than the aggregated contribution of wood products in use and in landfills (including industrial waste), which reinforces the role that wood products play in national carbon budgets

  20. Rethinking Eucalyptus globulus Labill. Based Land Use Systems in Smallholder Farmers Livelihoods: A Case of Kolobo Watershed, West Shewa, Ethiopia

    Directory of Open Access Journals (Sweden)

    Feyisa Dadi

    2018-03-01

    Full Text Available Despite their restriction, smallholder farmers have been continuing growing Eucalyptus globulus in the cultivated land in the central highland of Ethiopia. Literature has shown controversial issues against E. globulus. Therefore, the objective of the study was to investigate the compatibility of E. globulus in the smallholder farmers’ land use system. Soil samples were collected from five different land uses and analysed for selected physical and chemical properties. The socioeconomic contribution of E. globulus was collected through household surveys from 110 households. Analysis of soil showed that organic carbon (OC, total nitrogen (TN and cation exchange capacity (CEC were significantly higher (P<0.05 under E. globulus compared to the cultivated land. The survey results also showed that the largest proportion (58% of households was interested in growing E. globulus because of its multiple uses. About 83% of households responded that E. globulus help them to attain food security through increasing the purchasing power of smallholder farmers to buy agricultural inputs and food. This study has substantiated the role of E. globulus in the land use system of smallholder farmers. Most of the soil fertility indicators were better under E. globulus. The present finding reveals that E. globulus degrade the soil seemingly difficult to generalise. Growing E. globulus must be promoted under appealing land use to enhance smallholder farmers’ livelihoods. Removing E. globulus from the land use system may jeopardise the food security situation of many households.

  1. BAYESIAN PREDICTION OF GENETIC PARAMETERS IN Eucalyptus globulus CLONES UNDER WATER SUPPLY CONDITIONS

    Directory of Open Access Journals (Sweden)

    Freddy Mora

    2013-06-01

    Full Text Available http://dx.doi.org/10.5902/198050989297A Bayesian analysis of genetic parameters for growth traits at twelve months after planting was carried out in twenty nine Eucalyptus globulus clones in southern Chile. Two different environmental conditions were considered: 1 Non-irrigation and; 2 Plants were irrigated with a localized irrigation system. The Bayesian approach was performed using Gibbs sampling algorithm in a clone-environment interaction model. Inheritability values ​​were high in the water supply condition (posterior mode: H2=0.41, 0.36 and 0.39 for height, diameter and sectional area, respectively, while in the environment without irrigation, the inheritabilities were significantly lower, which was confirmed by the Bayesian credible intervals (95% probability. The posterior mode of the genetic correlation between sites was positive and high for all traits (r=0.7, 0.65 and 0.8, for height, diameter and sectional area, respectively and according to the credible interval, it was statistically different from zero, indicating a non-significant interaction.

  2. Allelopathic effect of aqueous extracts of Eucalyptus globulus Labill. and of Casearia sylvestris Sw. on cropsEfeito alelopático de extratos aquosos de Eucalyptus globulus Labill. e de Casearia sylvestris Sw. sobre espécies cultivadas

    Directory of Open Access Journals (Sweden)

    Grasielle Soares Gusman

    2011-10-01

    Full Text Available Allelopathy is characterized by the harmful or benefic effects caused by secondary metabolites, that are produced by plants, microorganisms or fungi and are released in the environment, on the development of natural biological systems or implemented ones. This study aimed to evaluate the allelopathic effects of aqueous extracts of eucalypt (Eucalyptus globulus Labill. and wild coffee (Casearia sylvestris Sw. on the germination and initial development of mustard (Brassica campestris L., cabbage (Brassica oleracea L. cv. capitata, broccoli (Brassica oleracea L. cv. italica, kale (Brassica pekinensis L., lettuce (Lactuca sativa L. cv. grand rapids, tomato (Lycopersicum esculentum Miller, turnip (Brassica rapa L., rucola (Eruca sativa L. and radish (Raphanus sativus L.. Six concentrations of each aqueous extract were tested (10, 30, 50, 70, 90 and 100% and compared to control (distilled water, with five replicates of each concentration, being ten seeds of each crop distributed in each replicate. The aqueous extracts of E. globulus and C. sylvestris reduced significantly the percentage of seed germination, the index of germination speed and the initial growth of the above ground part and roots of all cultivated species, being the reduction of these parameters higher with the increment of the aqueous extracts concentration, which led to thicker and atrophied roots with a higher number of absorbent hairs. Therefore, the results indicate an existence of allelopathic potential of E. globulus and C. sylvestris.A alelopatia caracteriza-se pelos efeitos danosos ou benéficos que metabólitos secundários produzidos por plantas, microrganismos ou fungos liberados no ambiente exercem sobre o desenvolvimento de sistemas biológicos naturais ou implantados. O objetivo do trabalho foi avaliar o efeito alelopático de extratos aquosos de eucalipto (Eucalyptus globulus Labill. e guaçatonga (Casearia sylvestris Sw. na germinação e no crescimento inicial de

  3. Organic matter production in an age series of Eucalyptus globulus plantations in Tamil Nadu

    Energy Technology Data Exchange (ETDEWEB)

    Negi, J D.S.; Bora, N K.S.; Tandon, V N; Thapliyal, H D

    1984-08-01

    The distribution of organic matter in an age series of Eucalyptus globulus plantations in Tamil Nadu is discussed. The total biomass ranges from 38 tonnes (5 years) to 220 tonnes (16 years) per ha with 85 to 88 percent being contributed by the aboveground parts and 15 to 12 percent by the roots and the average annual production of non-photosynthetic components is at its peak (19 tonnes/ha) at the age of 7 years. 17 references, 4 tables.

  4. Antifungal and antimycotoxigenic activity of essential oils from Eucalyptus globulus, Thymus capitatus and Schinus molle

    Directory of Open Access Journals (Sweden)

    Ana Karenth LÓPEZ-MENESES

    2015-12-01

    Full Text Available Abstract Essential oils (EO of eucalyptus (Eucalyptus globulus L., thymus (Thymus capitatus L. pirul (Schinus molle L. were evaluated for their efficacy to control Aspergillus parasiticus and Fusarium moniliforme growth and their ability to produce mycotoxins. Data from kinetics radial growth was used to obtain the half maximal inhibitory concentration (IC50. The IC50 was used to evaluate spore germination kinetic and mycotoxin production. Also, spore viability was evaluated by the MTT assay. All EO had an effect on the radial growth of both species. After 96 h of incubation, thymus EO at concentrations of 1000 and 2500 µL L–1 totally inhibited the growth of F. moniliforme and A. parasiticus, respectively. Eucalyptus and thymus EO significantly reduced spore germination of A. parasiticus. Inhibition of spore germination of F. moniliforme was 84.6, 34.0, and 30.6% when exposed to eucalyptus, pirul, and thymus EO, respectively. Thymus and eucalyptus EO reduced aflatoxin (4% and fumonisin (31% production, respectively. Spore viability was affected when oils concentration increased, being the thymus EO the one that reduced proliferation of both fungi. Our findings suggest that EO affect F. moniliforme and A. parasiticus development and mycotoxin production.

  5. Yield models for Eucalyptus globulus fuelwood plantations in Ethiopia

    Energy Technology Data Exchange (ETDEWEB)

    Pukkala, T.; Pohjonen, V. (Joensuu Univ. (FI). Faculty of Forestry)

    1990-01-01

    Based on 53 tree analyses and 105 sample plots of Eucalyptus globulus, models for volume and biomass at single tree and stand levels were developed. The possible growing sites were divided into four site classes. In seedling stands, the site class I corresponds to yield class 44 m{sup 3} ha{sup -1} year{sup -1}, in coppice stands to yield class 46 m{sup 3} ha{sup -1} year{sup -1}. The site class IV corresponds in seedling stand to yield class 9 m{sup 3} ha{sup -1} year{sup -1}, in coppice stands to yield class 13 m{sup 3} ha{sup -1} year{sup -1}. The maximum mean annual increment was reached in seedling stands at the age of 18-19 years, in coppice stands at the age of 14 years. (author).

  6. Steam torrefaction of Eucalyptus globulus for producing black pellets: A pilot-scale experience.

    Science.gov (United States)

    Arteaga-Pérez, Luis E; Grandón, Héctor; Flores, Mauricio; Segura, Cristina; Kelley, Stephen S

    2017-08-01

    Steam torrefaction of Eucalyptus globulus was performed at temperatures between 245°C and 265°C in a 100kg/h pilot plant. Torrefied biomass was then pelletized in a 300kg/h unit and the pellets were subject to durability, density and combustion tests. The structural changes measured with FTIR were studied along with the combustion behavior of the materials. Compositional analysis showed that increasing the torrefaction temperature reduced both hemicellulose fraction and overall mass yield (MY). Furthermore, there was a linear relationship between the energy yield (EY) and mass yield (EY=[1.04-0.9(1-MY)]) for these samples. The ignition and comprehensive indexes confirmed that the stability of the torrefied biomass in a combustion environment was higher than for untreated biomass. Finally, pellets showed high durability (98%), and had an energy density (13-14GJ/m 3 ), which is comparable to low-rank coals. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Torrefaction of wood and bark from Eucalyptus globulus and Eucalyptus nitens: Focus on volatile evolution vs feasible temperatures

    International Nuclear Information System (INIS)

    Arteaga-Pérez, Luis E.; Segura, Cristina; Bustamante-García, Verónica; Gómez Cápiro, Oscar; Jiménez, Romel

    2015-01-01

    Torrefaction is a thermal pretreatment leading to the improvement of most of the fuel properties of biomass, namely energy density, HHV (higher heating value), grindability and hydrophobicity. The aim of this study is to identify the most feasible temperature to carry out torrefaction of Eucalyptus globulus and nitens, based on chemical evidences associated to the release of volatiles during thermal treatment of biomass. With that end: (i) Devolatilization kinetics, (ii) Effects of temperature and residence time and (iii) volatiles composition during torrefaction of both wood and bark were analyzed. In all cases DTG (derivative thermogravimetric curves) exhibited the typical shape of lignocellulosic materials, with three decomposition phases and two reaction zones. Values of activation energies for hemicellulose decomposition, were in agreement with those reported in the literature (121–170 kJ/mol). Carboxylic acids, water and phenolic compounds showed two peaks, which were associated to torrefaction (below 310 °C) and pyrolysis (310–410 °C) respectively. The most feasible temperatures for torrefaction were estimated as a function of these peaks, and it ranged between 295 °C and 310°C for all samples. Main volatile species at the torrefaction peaks were distributed as Water > Acetic Acid > CO_2 > Others, while Levoglucosan formation was marginal, due to the catalytic effect of inorganics. - Highlights: • Identification of torrefaction peaks for E. globulus and E. nitens using dynamic TGA-MS. • Devolatilization kinetics of E. globulus and E. nitens (wood and bark). • Effect of operation parameters on torrefaction of E. globulus and E. nitens. • Most feasible temperatures for torrefaction based on chemical and thermal evidences. • Definition of atomic indicator to estimate degradation degree based on TGA-MS.

  8. Linking photosynthesis and leaf N allocation under future elevated CO2 and climate warming in Eucalyptus globulus

    Science.gov (United States)

    Sharwood, Robert E.; Crous, Kristine Y.; Whitney, Spencer M.; Ellsworth, David S.

    2017-01-01

    Abstract Leaf-level photosynthetic processes and their environmental dependencies are critical for estimating CO2 uptake from the atmosphere. These estimates use biochemical-based models of photosynthesis that require accurate Rubisco kinetics. We investigated the effects of canopy position, elevated atmospheric CO2 [eC; ambient CO2 (aC)+240 ppm] and elevated air temperature (eT; ambient temperature (aT)+3 °C) on Rubisco content and activity together with the relationship between leaf N and Vcmax (maximal Rubisco carboxylation rate) of 7 m tall, soil-grown Eucalyptus globulus trees. The kinetics of E. globulus and tobacco Rubisco at 25 °C were similar. In vitro estimates of Vcmax derived from measures of E. globulus Rubisco content and kinetics were consistent, although slightly lower, than the in vivo rates extrapolated from gas exchange. In E. globulus, the fraction of N invested in Rubisco was substantially lower than for crop species and varied with treatments. Photosynthetic acclimation of E. globulus leaves to eC was underpinned by reduced leaf N and Rubisco contents; the opposite occurred in response to eT coinciding with growth resumption in spring. Our findings highlight the adaptive capacity of this key forest species to allocate leaf N flexibly to Rubisco and other photosynthetic proteins across differing canopy positions in response to future, warmer and elevated [CO2] climates. PMID:28064178

  9. Water stress and recovery in the performance of two Eucalyptus globulus clones: physiological and biochemical profiles.

    Science.gov (United States)

    Correia, Barbara; Pintó-Marijuan, Marta; Neves, Lucinda; Brossa, Ricard; Dias, Maria Celeste; Costa, Armando; Castro, Bruno B; Araújo, Clara; Santos, Conceição; Chaves, Maria Manuela; Pinto, Glória

    2014-04-01

    Eucalyptus plantations are among the most productive forest stands in Portugal and Spain, being mostly used for pulp production and, more recently, as an energy crop. However, the region's Mediterranean climate, with characteristic severe summer drought, negatively affects eucalypt growth and increases mortality. Although the physiological response to water shortage is well characterized for this species, evidence about the plants' recovery ability remains scarce. In order to assess the physiological and biochemical response of Eucalyptus globulus during the recovery phase, two genotypes (AL-18 and AL-10) were submitted to a 3-week water stress period at two different intensities (18 and 25% of field capacity), followed by 1 week of rewatering. Recovery was assessed 1 day and 1 week after rehydration. Drought reduced height, biomass, water potential, NPQ and gas exchange in both genotypes. Contrarily, the levels of pigments, chlorophyll fluorescence parameters (F(v) /F(m) and (φPSII)), MDA and ABA increased. During recovery, the physiological and biochemical profile of stressed plants showed a similar trend: they experienced reversion of altered traits (MDA, ABA, E, g(s), pigments), while other parameters did not recover ((φPSII), NPQ). Furthermore, an overcompensation of CO(2) assimilation was achieved 1 week after rehydration, which was accompanied by greater growth and re-establishment of oxidative balance. Both genotypes were tolerant to the tested conditions, although clonal differences were found. AL-10 was more productive and showed a more rapid and dynamic response to rehydration (namely in carotenoid content, (φPSII) and NPQ) compared to clone AL-18. © 2013 Scandinavian Plant Physiology Society.

  10. RECOVERY OF LOGGING RESIDUES THROUGH BALER ON FORWARDER: EXPERIENCE WITH Eucalyptus globulus IN CHILE

    Directory of Open Access Journals (Sweden)

    Eduardo Acuña

    2018-05-01

    Full Text Available ABSTRACT This study presents results of tests on the recovery of Eucalyptus globulus logging residues. Leftovers were packed with an ENFO-2002 baler on a forwarder, taking advantage of a cut-to-length harvesting system for two conditions of residual biomass, i.e., with and without bark. A continuous field study was carried out, controlling 16.4 scheduled hours in seven days. Delays in the operation stemmed mainly by the visits of the client company and supervision by phone. The baler had an average utilization capacity of 81% for both evaluated conditions and produced an average of 36.2 bales with and 22.2 bales without bark per productive area. An average dry biomass productivity of 5.85 and 5.46 Mg h-1 was reached for similar conditions of residual biomass. Significant differences emerged when the type of harvesting was compared to mean dry weight, between types of harvesting, and mean baling time. Thus, the presence of bark in the logging residues of E. globulus increases the productivity of the baler.

  11. Efeito do óleo essencial de Eucalyptus globulus sobre espécies produtoras de aflatoxinas

    OpenAIRE

    Georgia Rocha Vilela

    2007-01-01

    Há relatos na literatura de alguns compostos naturais de plantas que são usados para preservação de alimentos e no controle do desenvolvimento de fungos e bactérias que ocorrem em plantas, grãos, cereais e derivados. O presente trabalho teve como objetivo avaliar "in vitro" o efeito do óleo essencial de Eucalyptus globulus e seu composto majoritário sobre o crescimento micelial dos fungos Aspergillus flavus e Aspergillus parasiticus e a produção de aflatoxinas. A composição química do...

  12. Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production

    Science.gov (United States)

    Domingues, Rui M. A.; Oliveira, Eduardo L. G.; Freire, Carmen S. R.; Couto, Ricardo M.; Simões, Pedro C.; Neto, Carlos P.; Silvestre, Armando J. D.; Silva, Carlos M.

    2012-01-01

    Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids). In this work, the supercritical fluid extraction (SFE) of Eucalyptus globulus deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100–200 bar), co-solvent (ethanol) content (0, 5 and 8% wt), and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by several tens of bar. At 160 bar and 40 °C, the introduction of 8% (wt) of ethanol greatly improves the yield of triterpenoids more than threefold. PMID:22837719

  13. Effet de l'hybridation interspecifique sur la teneur et la composition chimique des huiles essentielles d'eucalyptus cultivés au Maroc

    OpenAIRE

    Farah A.; Fechtal M.; Chaouch A.

    2002-01-01

    Interspecific hybridization effect on the content and the chemical composition of essential oils of eucalyptus grown in Morocco. In this work, the quality and quantity of the leave essential oils of five Eucalyptus hybrids were determined. The crosses of Eucalyptus globulus ssp. maideni, Eucalyptus globulus ssp. globulus, Eucalyptus grandis, Eucalyptus cladocalyx and Eucalyptus diversicolor with Eucalyptus camaldulensis have been studied. The average yield of parental species samples varies a...

  14. Ecophysiological responses of a young blue gum (Eucalyptus globulus) plantation to weed control.

    Science.gov (United States)

    Eyles, Alieta; Worledge, Dale; Sands, Peter; Ottenschlaeger, Maria L; Paterson, Steve C; Mendham, Daniel; O'Grady, Anthony P

    2012-08-01

    Early weed control may improve the growth of forest plantations by influencing soil water and nutrient availability. To understand eucalypt growth responses to weed control, we examined the temporal responses of leaf gas-exchange, leaf nitrogen concentration (N) and water status of 7-month-old Eucalyptus globulus L. trees in a paired-plot field trial. In addition, we monitored the growth, leaf N and water status of the competing vegetation in the weed treatment. By the end of the 11-month experiment, complete weed control (WF treatment) of largely woody competitors increased the basal diameter of E. globulus by 14%. As indicated by pre-dawn water potentials of > - 0.05 MPa, interspecies competition for water resources was minimal at this site. In contrast, competition for N appeared to be the major factor limiting growth. Estimations of total plot leaf N (g m(-2) ground) showed that competing vegetation accounted for up to 70% of the total leaf N at the start of the trial. This value fell to 15% by the end of the trial. Despite increased leaf N(area) in WF trees 5 months after imposition of weed control, the photosynthetic capacity (A(1500)) of E. globulus was unaffected by treatment suggesting that the growth gains from weed control were largely unrelated to changes in leaf-level photosynthesis. Increased nutrient availability brought about by weed control enabled trees to increase investment into leaf-area production. Estimates of whole-tree carbon budget based on direct measurements of dark respiration and A(1500) allowed us to clearly demonstrate the importance of leaf area driving greater productivity following early weed control in a nutrient-limited site.

  15. CARACTERIZACION DE GENES DESHIDRINA EN EUCALYPTUS GLOBULUS CUYA EXPRESION ES INDUCIDA POR LA ACLIMATACION A BAJA TEMPERATURA

    OpenAIRE

    FERNANDEZ REYES, MARTA ALEJANDRA

    2011-01-01

    Eucalyptus globulus es una especie importante para la producción de pulpa de madera en Chile, sin embargo presenta una gran sensibilidad a temperaturas de congelamiento. Durante los últimos años, muchos estudios han enfocado sus esfuerzos en dilucidar los mecanismos que regulan la respuesta de las plantas a baja temperatura, mediante el análisis de la expresión génica. En el presente trabajo se estudia la expresión de genes deshidrina durante el proceso de aclimatación a baja temperatura y...

  16. CARACTERIZACION DE GENES DESHIDRINA EN EUCALYPTUS GLOBULUS CUYA EXPRESION ES INDUCIDA POR LA ACLIMATACION A BAJA TEMPERATURA

    OpenAIRE

    FERNANDEZ REYES ; MARTA ALEJANDRA; FERNANDEZ REYES ; MARTA ALEJANDRA

    2011-01-01

    Eucalyptus globulus es una especie importante para la producción de pulpa de madera en Chile, sin embargo presenta una gran sensibilidad a temperaturas de congelamiento. Durante los últimos años, muchos estudios han enfocado sus esfuerzos en dilucidar los mecanismos que regulan la respuesta de las plantas a baja temperatura, mediante el análisis de la expresión génica. En el presente trabajo se estudia la expresión de genes deshidrina durante el proceso de aclimatación a baja temperatura y la...

  17. 27-36, 2014 27 Quality assessment of essential oils of Eucalyptus

    African Journals Online (AJOL)

    In the course of this study, essential oils of Eucalyptus globulus and three Boswellia rivae species were analyzed using ... parts of the plant, including leaves, flowers, seeds, rhizomes ..... oil and its major component 1,8-cineole against fungal.

  18. Litter production and decomposition in Eucalyptus urophylla x Eucalyptus globulus maidenii stand

    Directory of Open Access Journals (Sweden)

    Mauro Valdir Schumacher

    2013-09-01

    Full Text Available he sustainable wood production in commercial plantations requires knowledge of the nutrient cycling process, which also involves the production and decomposition of litter. This study verified the influence of climatic variables on litter production and t evaluated the rate of leaf litter decomposition in a stand of Eucalyptus urophylla x E. globulus maidenii. There were installed 4 plots of 20 m x 20 m, in each plot four litter traps to collect leaves were placed, thin branches and miscellaneous, beside this, each plot received 3 areas for coarse branches collection. The litter collected was used to calculate the deposition and the correlation between climate variables and deposition. The climatic variables used, on a monthly basis, were average temperature, average maximum temperature, average minimum temperature, rainfall, relative humidity, average wind speed, average solar radiation and average evapotranspiration, both supplied by an experimental station. For evaluation of the litter decomposition rate, four square samples of 0.25 m side in each plot were randomly collected and used for determining the decay coefficient (K, half life (t0,5 and decomposition time of 95% of litter (t0,95 . The monthly litter production was weakly correlated with climatic variables and the annual production was 7.4 Mg ha-1, with leaves as the major fraction (60%. The litter decomposition rate was considered slow.

  19. EFFECT OF LIGNIN CONTENT OF Eucalyptus globulus WOOD IN KRAFT PULPING PERFORMANCE

    Directory of Open Access Journals (Sweden)

    Gabriel Valim Cardoso

    2011-03-01

    Full Text Available In this research, it was analyzed the lignin content effect of Eucalyptus globulus wood in kraft pulping optimization. Seventy-two laboratory cooking were made with wood chips obtained from six Eucalyptus globulus trees selected from a group of 50 trees. The wood chips from three trees with the lowest lignin content, with average 20,53%, were mixed proportionally based on the tree weights, obtaining the sample of low lignin content wood. The same was made to obtain the sample for wood chips with the highest lignin content, with average 23,02%. The two lignin levels were statistically different. The two wood samples had basic densities statistically not different. Using three maximum temperatures levels (160, 165 and 170ºC, and three active alkali charged (17, 18,5 and 20%, the wood chips were converted to kraft pulps. The pulps were then characterized to analyze the influence of the distinct treatments employed in the cooking on their properties. The effect of the cooking conditions was expressed by mathematical models in order to determine the optimum points for each of the evaluated properties. The optimization process indicated maximum temperature of 168ºC, and active alkali of 19%, for maximum kraft pulping yield to achieve kappa number 18; this result was for woods with low lignin content. For woods containing the high lignin content, the optimization showed maximum cooking temperature of 169ºC and active alkali of 19% for kappa number of 18. The average reduction of 2,49% in wood lignin content resulted a correspondent gain of 2,2% in the kraft yield (o.d. basis and a reduction on the active alkali charge of 1,2% (o.d. basis to achieve kappa numbers from 16 to 19, preserving pulp properties. If the option is to work with kappa number 19 instead of 16, the gain in kraft yield is approximately 2%. Therefore, when working with low lignin content wood and kappa number 19 instead of 16, a substantial gain of approximately 4,2% is obtained

  20. Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region

    Directory of Open Access Journals (Sweden)

    Paula eGuzmán

    2014-09-01

    Full Text Available The plant cuticle has traditionally been conceived as an independent hydrophobic layer that covers the external epidermal cell wall. Due to its complexity, the existing relationship between cuticle chemical composition and ultra-structure remains unclear to date. This study aimed to examine the link between chemical composition and structure of isolated, adaxial leaf cuticles of Eucalyptus camaldulensis and E. globulus by the gradual extraction and identification of lipid constituents (cutin and soluble lipids, coupled to spectroscopic and microscopic analyses. The soluble compounds and cutin monomers identified could not be assigned to a concrete internal cuticle ultra-structure. After cutin depolymerization, a cellulose network resembling the cell wall was observed, with different structural patterns in the regions ascribed to the cuticle proper and cuticular layer, respectively. Our results suggest that the current cuticle model should be revised, stressing the presence and major role of cell wall polysaccharides. It is concluded that the cuticle may be interpreted as a modified cell wall region which contains additional lipids. The major heterogeneity of the plant cuticle makes it difficult to establish a direct link between cuticle chemistry and structure with the existing methodologies.

  1. Enhancement of enzymatic saccharification of Eucalyptus globulus: steam explosion versus steam treatment.

    Science.gov (United States)

    Martin-Sampedro, Raquel; Revilla, Esteban; Villar, Juan C; Eugenio, Maria E

    2014-09-01

    Steam explosion and steam pre-treatment have proved capable of enhancing enzymatic saccharification of lignocellulosic materials. However, until now, these methods had not been compared under the same operational conditions and using the same raw material. Both pre-treatments lead to increased yields in the saccharification of Eucalyptus globulus; but results have been better with steam pre-treatments, despite the more accessible surface of exploded samples. The reason for this finding could be enzymatic inhibition: steam explosion causes a more extensive extraction of hemicelluloses and releases a greater amount of degradation products which can inhibit enzymatic action. Enzymatic inhibition is also dependent on the amount and chemical structure of lignin, which was also a contributing factor to the lower enzymatic yields obtained with the most severe pre-treatment. Thus, the highest yields (46.7% glucose and 73.4% xylose yields) were obtained after two cycle of steam treatment, of 5 and 3 min, at 183°C. Copyright © 2014 Elsevier Ltd. All rights reserved.

  2. Green Synthesis of Silver Nanoparticles by using Eucalyptus Globulus Leaf Extract

    Science.gov (United States)

    Balamurugan, Madheswaran; Saravanan, Shanmugam

    2017-12-01

    A single step eco-friendly, energy efficient and economically scalable green method was employed to synthesize silver nanoparticles. In this work, the synthesis of silver nanoparticles using Eucalyptus globulus leaf extract as reducing and capping agent along with water as solvent at normal room temperature is described. Silver nanoparticles were prepared from aqueous silver nitrate solution by adding the leaf extract. The prepared nanoparticles were characterized by using UV-visible Spectrophotometer, X-ray diffractometer, High Resolution Transmission Electron Microscope (HR-TEM) and Fourier Transform Infrared Spectroscope (FTIS). X-ray diffraction studies brought to light the crystalline nature and the face centered cubic structure of the silver nanoparticles. Using HR-TEM. the nano sizes and morphology of the particles were studied. The mean sizes of the prepared silver nanoparticles ranged from 30 to 36 nm. The density of the particles was tuned by varying the molar ratio of silver nitrate. FTIS studies showed the functional group of organic molecules which were located on the surface of the silver nanoparticles. Originating from the leaf extracts, these organic molecules reduced and capped the particles.

  3. Utilización de Eucalyptus spp. Alternativas de plantaciones uruguayas para pulpa Kraft

    Directory of Open Access Journals (Sweden)

    Javier Doldán

    2011-05-01

    Full Text Available Las plantaciones de Eucalyptus globulus han mostrado desiguales tasas de crecimiento en diferentes regiones de Uruguay. Esto ha motivado la búsqueda de otros orígenes de semilla y especies para la producción de pulpa de celulosa. Propiedades papeleras de las pulpas blanqueadas (ECF de especies alternativas llevan a intuir que podrían ser atractivas para mercados de pulpa de fibra corta. En este trabajo se realiza un comparativo del E. grandis, E. dunnii, E. maidenii y E. globulus (procedencia de semilla “Jeeralang” con el E. globulus predominante en Uruguay. Se discute el potencial de estas maderas como base para una mezcla en cocción, basándose en el análisis de propiedades físicas (densidad aparente básica, propiedades pulpables (rendimiento, carga de álcali activo en cocción Kraft y consumo de madera y propiedades papeleras. En trabajos previos se han encontrado diferencias significativas entre el comportamiento pulpable del Eucalyptus globulus y Eucalyptus maidenii, sugiriendo que nosería recomendable mezclar estas especies. La misma conclusión se podría extender a las especies de Eucalyptus estudiadas. Sin embargo, teniendo en cuenta los similares requerimientos en las cargas de álcali activo, la mezcla entre especies alternativas podría ser aplicada.AbstractEucalyptus globulus plantations have shown different growth rates in different sites in Uruguay. This fact has triggered the search for other pulp wood species and seed provenance. Paper making properties of ECF bleached pulps of alternative speciessuggest that these species could be perfectly used as hardwood bleached pulp raw materials. This study intends to compare alternativeUruguayan pulpwood species E. grandis, E. dunnii, E. maidenii and “Jeeralang” a seed provenance of E. globulus to the E. globulus most widely cultivated in the country. Physical properties of wood (Basic Density, Kraft pulping performance (pulp yield, active alkali and wood consumption

  4. MODELACIÓN DEL EFECTO DE LA PODA Y RALEO SOBRE LAS DEFORMACIONES RESIDUALES LONGITUDINALES EN Eucalyptus globulus Labill.

    OpenAIRE

    Eduardo Navarrete; Carolina Valenzuela; Leif Nutto

    2016-01-01

    RESUMEN Se estudió una plantación de Eucalyptus globulus ubicada en el sector precordillerano andino de la región del Bío Bío, Chile, c on el objetivo de evaluar y modelar el efecto de la poda y raleo sobre las deformaciones residuales longitudinales (DRL) . El lugar de estudio correspondió a un rodal de 12 años de edad, el cual fue intervenido a los 2 años y medio de establecido, donde se evaluaron distintas intensidades de poda (0% y 60% de la altura total) y densidades de raleo (1.6...

  5. MODELACIÓN DEL EFECTO DE LA PODA Y RALEO SOBRE LAS DEFORMACIONES RESIDUALES LONGITUDINALES EN Eucalyptus globulus Labill.

    OpenAIRE

    Navarrete, Eduardo; Valenzuela, Carolina; Nutto, Leif

    2016-01-01

    RESUMEN Se estudió una plantación de Eucalyptus globulus ubicada en el sector precordillerano andino de la región del Bío Bío, Chile, con el objetivo de evaluar y modelar el efecto de la poda y raleo sobre las deformaciones residuales longitudinales (DRL). El lugar de estudio correspondió a un rodal de 12 años de edad, el cual fue intervenido a los 2 años y medio de establecido, donde se evaluaron distintas intensidades de poda (0% y 60% de la altura total) y densidades de raleo (1.600 árb·ha...

  6. Comparative gas chromatography-mass spectroscopy, Fourier transform infrared spectroscopy, and high-performance liquid chromatography analysis of essential oils extracted using 4 methods from the leaves of Eucalyptus globulus L.

    Directory of Open Access Journals (Sweden)

    Yasmeen Khan

    2016-01-01

    Full Text Available Background: Eucalyptus globulus L. (family, Myrtaceae is one of the world′s most widely planted genera. E. globulus L., commonly referred to as Tasmanian blue gum, is a fast growing, evergreen tree, native to Tasmania and South-East Australia. Apart from its extensive use in pulp industry, it is also produces Oleum Eucalypti (eucalyptus oil that is extracted on commercial scale in many countries such as China, India, South Africa, Portugal, Brazil, and Tasmania, as a raw material in perfumery, cosmetics, food beverage, aromatherapy, and phytotherapy. Materials and Methods: Traditional hydrodistillation (HD, solvent extraction (SE, ultrasonication (US, and supercritical fluid extraction (SFE were conducted for the extraction of essential oil from the leaves of E. globulus. Each oil was evaluated in terms of high-performance liquid chromatography (HPTLC and Fourier transform infrared spectroscopy (FTIR fingerprinting with qualitative and semi-quantitative composition of the isolated essential oil by gas chromatography-mass spectroscopy (GCMS, the extract yield of essential oil was 2.60%, 2.2%, 2.0%, and 3.6% v/w, respectively, for HD, SE, US, and SFE. Results: A total of 53 compounds were identified by GCMS. Comparative analysis indicated that SFE was favorable for extraction of monoterpene hydrocarbon, sesquiterpene hydrocarbon, and oxygenated sesquiterpene hydrocarbon. HD, SE, and US had certain advantages in the extraction of aliphatic saturated hydrocarbons organic acid and esters. Overlay, FTIR spectra of oil samples obtained by four extraction methods were superimposed with each other showing similar components. The maximum separation of compound seen at 254 nm and lesser at 366 nm by HPTLC fingerprinting which again showed superimposed chromatograms. Conclusion: It is concluded that different extraction method may lead to different yields of essential oils where the choice of appropriate method is very important to obtained more desired

  7. Reference gene selection for quantitative reverse transcription-polymerase chain reaction normalization during in vitro adventitious rooting in Eucalyptus globulus Labill.

    Science.gov (United States)

    de Almeida, Márcia R; Ruedell, Carolina M; Ricachenevsky, Felipe K; Sperotto, Raul A; Pasquali, Giancarlo; Fett-Neto, Arthur G

    2010-09-20

    Eucalyptus globulus and its hybrids are very important for the cellulose and paper industry mainly due to their low lignin content and frost resistance. However, rooting of cuttings of this species is recalcitrant and exogenous auxin application is often necessary for good root development. To date one of the most accurate methods available for gene expression analysis is quantitative reverse transcription-polymerase chain reaction (qPCR); however, reliable use of this technique requires reference genes for normalization. There is no single reference gene that can be regarded as universal for all experiments and biological materials. Thus, the identification of reliable reference genes must be done for every species and experimental approach. The present study aimed at identifying suitable control genes for normalization of gene expression associated with adventitious rooting in E. globulus microcuttings. By the use of two distinct algorithms, geNorm and NormFinder, we have assessed gene expression stability of eleven candidate reference genes in E. globulus: 18S, ACT2, EF2, EUC12, H2B, IDH, SAND, TIP41, TUA, UBI and 33380. The candidate reference genes were evaluated in microccuttings rooted in vitro, in presence or absence of auxin, along six time-points spanning the process of adventitious rooting. Overall, the stability profiles of these genes determined with each one of the algorithms were very similar. Slight differences were observed in the most stable pair of genes indicated by each program: IDH and SAND for geNorm, and H2B and TUA for NormFinder. Both programs identified UBI and 18S as the most variable genes. To validate these results and select the most suitable reference genes, the expression profile of the ARGONAUTE1 gene was evaluated in relation to the most stable candidate genes indicated by each algorithm. Our study showed that expression stability varied between putative reference genes tested in E. globulus. Based on the AGO1 relative expression

  8. Transfer Rates of 238U and 232Th for E. globulus, A. mearnsii, H. filipendula and Hazardous Effects of the Usage of Medicinal Plants From Around Gold Mine Dump Environs

    Directory of Open Access Journals (Sweden)

    Victor M. Tshivhase

    2015-12-01

    Full Text Available Medicinal plant consumption can be a source of human exposure to radioactive elements such as 238U and 232Th, which can lead to internal radiation doses. The uptake of 238U and 232Th from soils to the leaf samples of three different medicinal plant species (Eucalyptus globulus, Acacia mearnsii and Hyparrhenia filipendula from the purlieu of the Princess gold mine dump, an abandoned contaminated tailings storage site (TSS, located at longitude 27°55′00″E and latitude 26°09′30″S in Davidsonville (Roodepoort, west of Johannesburg, South Africa was measured. This was done using ICP-MS spectrometry and substantial differences were observed in the soil-plant transfer factor (TF values between these radionuclides. The plant species E. globulus exhibited the highest uptake of 238U, with an average TF of 3.97, while that of H. filipendula was 0.01 and the lowest TF of 0.15 × 10−2 was measured for A. mearnsii. However, in the case of 232Th, the highest average TF was observed for A. mearnsii (0.29, followed by E. globulus (0.10 and lowest was measured for H. filipendula (0.27 × 10−2. The ratio of TF average value i.e., 238U to 232Th in the soil-plant leaves was 38.05 for E. globulus, 0.01 for A. mearnsii and 4.38 for H. filipendula.

  9. Transfer Rates of 238U and 232Th for E. globulus, A. mearnsii, H. filipendula and Hazardous Effects of the Usage of Medicinal Plants From Around Gold Mine Dump Environs

    Science.gov (United States)

    Tshivhase, Victor M.; Njinga, Raymond L.; Mathuthu, Manny; Dlamini, Thulani C.

    2015-01-01

    Medicinal plant consumption can be a source of human exposure to radioactive elements such as 238U and 232Th, which can lead to internal radiation doses. The uptake of 238U and 232Th from soils to the leaf samples of three different medicinal plant species (Eucalyptus globulus, Acacia mearnsii and Hyparrhenia filipendula) from the purlieu of the Princess gold mine dump, an abandoned contaminated tailings storage site (TSS), located at longitude 27°55′00″E and latitude 26°09′30″S in Davidsonville (Roodepoort, west of Johannesburg, South Africa) was measured. This was done using ICP-MS spectrometry and substantial differences were observed in the soil-plant transfer factor (TF) values between these radionuclides. The plant species E. globulus exhibited the highest uptake of 238U, with an average TF of 3.97, while that of H. filipendula was 0.01 and the lowest TF of 0.15 × 10−2 was measured for A. mearnsii. However, in the case of 232Th, the highest average TF was observed for A. mearnsii (0.29), followed by E. globulus (0.10) and lowest was measured for H. filipendula (0.27 × 10−2). The ratio of TF average value i.e., 238U to 232Th in the soil-plant leaves was 38.05 for E. globulus, 0.01 for A. mearnsii and 4.38 for H. filipendula. PMID:26690462

  10. MINERAL ELEMENTS IN WOODS OF EUCALYPTUS AND BLACK WATTLE AND ITS INFLUENCE IN A BLEACHED KRAFT PULP MILL

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    André Fredo

    2009-09-01

    Full Text Available Effluents are one of the most important problems in a pulp mill regarding to environmental subjects. With the purpose to reduce them, the mills are closing the internal cycles and reducing the water consumption. The wood, as the most significant source of non-process elements to the system, is responsible for some troubles to the industrial process. With the aim of evaluating their intake and to offer some informations for closing the loop, the contents of Al, Ca, Cu, Fe, K, Mg, Mn, Na, Ni, and Si were analysed in Acacia mearnsii, Eucalyptus dunnii, Eucalyptus globulus globulus, Eucalyptus grandis and Eucalyptus saligna woods. Wood samples were prepared by oven burning and acidic digestion methods, following analysis by ICP (inductively coupled plasm. Using also the results from silvicultural evaluation of trees and stands, they were calculated which elements were carried out from the site in larger amounts (K, Ca, Na, Al, Mn and Si and the species which exported largest amount of these elements (Eucalyptus dunnii and Eucalyptus globulus. The species with lower growth were Eucalyptus globulus and Eucalyptus dunnii, that leads to a bigger elements input and more potential industrial troubles. It was observed a range of 3.8 (Eucalyptus grandis up to 6 (Eucalyptus dunnii kg of analised mineral elements introduced to the process per ton of umbleached pulp produced. The Acacia mearnsii showed the lower level for Fe, Mn and Ni, being useful for oxygen, ozone and peroxide bleaching. The silicon observed in woods was in low concentration although the high values of this element in industrial liquor cycle. This leads to state that there is some contamination with soil when harvesting and handling the wood. Special care must be taken with both high ash and high mineral elements species, such as Eucalyptus dunnii and Eucalyptus globulus.

  11. Deposição de serapilheira e nutrientes em plantio de Eucalyptus urophylla × E. globulus

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    Márcio Viera

    Full Text Available Objetivou-se com o presente estudo avaliar a deposição de serapilheira e nutrientes em plantio de Eucalyptus urophylla × Eucalyptus globulus, em Eldorado do Sul-RS. Foram alocadas sistematicamente quatro parcelas de 20 m × 20 m, cada uma com quatro coletores de serapilheira de 1 m². A serapilheira foi coletada quinzenalmente, entre janeiro/2007 e dezembro/2010. A produção de serapilheira foi crescente, com o aumento da idade do povoamento, sendo 6,9 Mg ha-1 aos seis e 8,5 Mg ha-1 aos nove anos. A fração folhas correspondeu, em média, a 66,9% da serapilheira total, seguida pelos galhos finos (14,7%, miscelânea (10,5% e galhos grossos (7,9%. A deposição apresentou padrão sazonal, sendo maior no período de aumento da temperatura do ar. A fração folhas foi responsável por maior parte do retorno de nutrientes via serapilheira, variando de 58,2 a 81,8% da quantidade de Cu e N, respectivamente.

  12. Targeting oxidative stress, acetylcholinesterase, proinflammatory cytokine, dopamine and GABA by eucalyptus oil (Eucalyptus globulus) to alleviate ketamine-induced psychosis in rats.

    Science.gov (United States)

    Yadav, Monu; Jindal, Deepak Kumar; Parle, Milind; Kumar, Anil; Dhingra, Sameer

    2018-02-20

    Essential oil of eucalyptus species is among the most common traded essential oils in the world. There is an increasing interest in the application of eucalyptus oil as a natural additive in food and pharmaceutical industry. The present study was undertaken to identify the phytoconstituents present in the essential oil of Eucalyptus globulus leaves (EO) and ascertain their protective effect against ketamine-induced psychosis in rats. GC-MS technique was used for analysis of phytoconstituents present in EO. Ketamine (50 mg/kg, i.p.) was used to induce psychosis in rats. Photoactometer, forced swim test and pole climb avoidance test were used to evaluate the protective effects of the EO (500, 1000 and 2000 mg/kg, p.o.) on acute and chronic administration. Bar test was used to test the side effect of EO. Biochemical and neurochemical estimations were carried out to explore the possible mechanism of action. GC-MS analysis of EO showed the presence of a number of biologically active compounds. EO at the dose of 500, 1000 and 2000 mg/kg, p.o. on acute and chronic administration, decreased locomotor activity, immobility duration and latency to climb the pole. EO was effective to facilitate the release of GABA, increase GSH levels, inhibit dopamine neurotransmission and decrease TNF-α levels as well as diminish AChE activity in different regions of the brain. EO at the dose of 500, 1000 mg/kg did not produce cataleptic behavior in rats. EO at the dose of 500, 1000 mg/kg produced protective effects against ketamine-induced psychosis and can be further explored clinically against neuropsychiatric disorders.

  13. COMPORTAMENTO ESPECTRAL DE FOLHAS DE Eucalyptus globulus (Labill. ATACADAS POR Mycosphaerella spp. NAS REGIÕES DO VISÍVEL E DO INFRAVERMELHO PRÓXIMO DO ESPECTRO ELETROMAGNÉTICO

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    Diogo Belmonte Lippert

    2015-01-01

    Full Text Available The study aims to analyze the spectral behavior of leaves of Eucalyptus globulus attacked in four different severity levels by Mycosphaerella spp. in different positions in the canopy of the tree in the four seasons, considering two regions of the electromagnetic spectrum: visible and near infrared. The plant material was collected in all seasons, in different positions in the tree (base, middle and top of the canopy extract. In the laboratory, leaves were classified into four levels of severity (healthy, little, medium and heavy fire and then made measurements of spectral reflectance measured with a spectroradiometer. The spectral behavior of Eucalyptus globules leaves is characteristic according to the severity degree of the disease, differing primarily in the other high levels of infection by Mycosphaerella spp. and differ mainly of healthy leaves and leaves which are little attacked, in both regions of the electromagnetic spectrum, this behavior occurs for different treetop positions and climate seasons, varying the amount of reflected energy. It is concluded that the reflectance of the Eucalyptus globules leaves attacked by Mycosphaerella spp. differs according to the position in the tree crown and the weather station when they were collected and to the advanced levels of disease severity.

  14. Methodology for logistic planning of forest roads for harvesting Eucalyptus globulus Labill. using optimization tools

    International Nuclear Information System (INIS)

    Leal Robert, Orlando; Lopez, Cesar Amilcar

    2011-01-01

    Using an un capacitated and non directed network model, we propose a methodology for the preliminary design of second order forests roads to extract products from Eucalyptus globulus plantations using an aerial cable system to minimize road network construction costs. The model takes into account topographical conditions and road construction costs related to the terrain slope. In order to solve the problem the Dijstrak algorithm was used, in which the minimum cost of the shortest paths that connect all the stockyards are defined. In addition, the Kruskal algorithm was used to obtain the minimum spanning tree. Finally, the network was connected to an open road for the timber to be taken to consumption centers. In order to develop the algorithms mentioned above, an application on Mat lab was designed, which gave an easier handling of the cartography data obtained from these algorithms.

  15. Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production

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    Carlos M. Silva

    2012-06-01

    Full Text Available Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids. In this work, the supercritical fluid extraction (SFE of Eucalyptus globulus deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100–200 bar, co-solvent (ethanol content (0, 5 and 8% wt, and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by several tens of bar. At 160 bar and 40 °C, the introduction of 8% (wt of ethanol greatly improves the yield of triterpenoids more than threefold.

  16. Defense responses in plants of Eucalyptus elicited by Streptomyces and challenged with Botrytis cinerea.

    Science.gov (United States)

    Salla, Tamiris D; Astarita, Leandro V; Santarém, Eliane R

    2016-04-01

    Elicitation of E. grandis plants with Streptomyces PM9 reduced the gray-mold disease, through increasing the levels of enzymes directly related to the induction of plant defense responses, and accumulation of specific phenolic compounds. Members of Eucalyptus are economically important woody species, especially as a raw material in many industrial sectors. Species of this genus are susceptible to pathogens such as Botrytis cinerea (gray mold). Biological control of plant diseases using rhizobacteria is one alternative to reduce the use of pesticides and pathogen attack. This study evaluated the metabolic and phenotypic responses of Eucalyptus grandis and E. globulus plants treated with Streptomyces sp. PM9 and challenged with the pathogenic fungus B. cinerea. Metabolic responses were evaluated by assessing the activities of the enzymes polyphenol oxidase and peroxidase as well as the levels of phenolic compounds and flavonoids. The incidence and progression of the fungal disease in PM9-treated plants and challenged with B. cinerea were evaluated. Treatment with Streptomyces sp. PM9 and challenge with B. cinerea led to changes in the activities of polyphenol oxidase and peroxidase as well as in the levels of phenolic compounds in the plants at different time points. Alterations in enzymes of PM9-treated plants were related to early defense responses in E. grandis. Gallic and chlorogenic acids were on average more abundant, although caffeic acid, benzoic acid and catechin were induced at specific time points during the culture period. Treatment with Streptomyces sp. PM9 significantly delayed the establishment of gray mold in E. grandis plants. These results demonstrate the action of Streptomyces sp. PM9 in inducing plant responses against B. cinerea, making this organism a potential candidate for biological control in Eucalyptus.

  17. Production of polyploids from cultured shoot tips of Eucalyptus ...

    African Journals Online (AJOL)

    Polyploids from cultured shoot tips of Eucalyptus globulus were produced by treatment with colchicine. Results showed that the combination of 0.5% colchicine and treating multiple shoot clumps for 4 days was the most appropriate conditions for E. globulus polyploidy induction and the effect of the use of multiple shoot ...

  18. Chemical Composition and in-Vitro Evaluation of the Antimicrobial and Antioxidant Activities of Essential Oils Extracted from Seven Eucalyptus Species.

    Science.gov (United States)

    Ghaffar, Abdul; Yameen, Muhammad; Kiran, Shumaila; Kamal, Shagufta; Jalal, Fatima; Munir, Bushra; Saleem, Sadaf; Rafiq, Naila; Ahmad, Aftab; Saba, Iram; Jabbar, Abdul

    2015-11-18

    Eucalyptus is well reputed for its use as medicinal plant around the globe. The present study was planned to evaluate chemical composition, antimicrobial and antioxidant activity of the essential oils (EOs) extracted from seven Eucalyptus species frequently found in South East Asia (Pakistan). EOs from Eucalyptus citriodora, Eucalyptus melanophloia, Eucalyptus crebra, Eucalyptus tereticornis, Eucalyptus globulus, Eucalyptus camaldulensis and Eucalyptus microtheca were extracted from leaves through hydrodistillation. The chemical composition of the EOs was determined through GC-MS-FID analysis. The study revealed presence of 31 compounds in E. citriodora and E. melanophloia, 27 compounds in E. crebra, 24 compounds in E. tereticornis, 10 compounds in E. globulus, 13 compounds in E. camaldulensis and 12 compounds in E. microtheca. 1,8-Cineole (56.5%), α-pinene (31.4%), citrinyl acetate (13.3%), eugenol (11.8%) and terpenene-4-ol (10.2%) were the highest principal components in these EOs. E. citriodora exhibited the highest antimicrobial activity against the five microbial species tested (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Aspergillus niger and Rhizopus solani). Gram positive bacteria were found more sensitive than Gram negative bacteria to all EOs. The diphenyl-1-picrylhydazyl (DPPH) radical scavenging activity and percentage inhibition of linoleic acid oxidation were highest in E. citriodora (82.1% and 83.8%, respectively) followed by E. camaldulensis (81.9% and 83.3%, respectively). The great variation in chemical composition of EOs from Eucalyptus, highlight its potential for medicinal and nutraceutical applications.

  19. Chemical Composition and in-Vitro Evaluation of the Antimicrobial and Antioxidant Activities of Essential Oils Extracted from Seven Eucalyptus Species

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    Abdul Ghaffar

    2015-11-01

    Full Text Available Eucalyptus is well reputed for its use as medicinal plant around the globe. The present study was planned to evaluate chemical composition, antimicrobial and antioxidant activity of the essential oils (EOs extracted from seven Eucalyptus species frequently found in South East Asia (Pakistan. EOs from Eucalyptus citriodora, Eucalyptus melanophloia, Eucalyptus crebra, Eucalyptus tereticornis, Eucalyptus globulus, Eucalyptus camaldulensis and Eucalyptus microtheca were extracted from leaves through hydrodistillation. The chemical composition of the EOs was determined through GC-MS-FID analysis. The study revealed presence of 31 compounds in E. citriodora and E. melanophloia, 27 compounds in E. crebra, 24 compounds in E. tereticornis, 10 compounds in E. globulus, 13 compounds in E. camaldulensis and 12 compounds in E. microtheca. 1,8-Cineole (56.5%, α-pinene (31.4%, citrinyl acetate (13.3%, eugenol (11.8% and terpenene-4-ol (10.2% were the highest principal components in these EOs. E. citriodora exhibited the highest antimicrobial activity against the five microbial species tested (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Aspergillus niger and Rhizopus solani. Gram positive bacteria were found more sensitive than Gram negative bacteria to all EOs. The diphenyl-1-picrylhydazyl (DPPH radical scavenging activity and percentage inhibition of linoleic acid oxidation were highest in E. citriodora (82.1% and 83.8%, respectively followed by E. camaldulensis (81.9% and 83.3%, respectively. The great variation in chemical composition of EOs from Eucalyptus, highlight its potential for medicinal and nutraceutical applications.

  20. MODELACIÓN DEL EFECTO DE LA PODA Y RALEO SOBRE LAS DEFORMACIONES RESIDUALES LONGITUDINALES EN Eucalyptus globulus Labill.

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    Eduardo Navarrete

    2016-01-01

    Full Text Available RESUMEN Se estudió una plantación de Eucalyptus globulus ubicada en el sector precordillerano andino de la región del Bío Bío, Chile, c on el objetivo de evaluar y modelar el efecto de la poda y raleo sobre las deformaciones residuales longitudinales (DRL . El lugar de estudio correspondió a un rodal de 12 años de edad, el cual fue intervenido a los 2 años y medio de establecido, donde se evaluaron distintas intensidades de poda (0% y 60% de la altura total y densidades de raleo (1.600 árb·ha -1 (sin raleo y 800 árb·ha -1 . En cada tratamiento definido por la combinación de los dos niveles de poda y raleo, se estudió además, el efecto de la clase de copa (dominante, codominante e intermedia sobre el comportamiento de las DRL. En cada unidad de muestreo se registraron variables a nivel de árbol individual, estimando los parámetros de rodal más relevantes. Se muestreó un total de 36 árboles, midiéndose en cada uno de ellos las DRL a nivel del Dap con un extensómetro diseñado por el CIRAD-Forêt. Los resultados determinaron que la poda y raleo no afectaron significativamente el comportamiento de las DRL en Eucalyptus globulus ; en cambio, la clase de copa, tuvo un efecto altamente significativo sobre la variación de las deformaciones residuales, presentando los árboles dominantes mayores niveles de deformación que las clases de copa codominante e intermedia, respectivamente. El modelo de estimación incluyó como variables predictivas del comportamiento de las DRL , las expresiones logarítmicas del Dap y del inverso multiplicativo de la varianza de los radios de copa, además de la poda, presentando un R 2 de 0,44 y un EEE de 0,132.

  1. Chemical characteristics and Kraft pulping of tension wood from Eucalyptus globulus labill Características químicas e polpação Kraft de madeira de tração de Eucalyptus globulus labill

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    María Graciela Aguayo

    2012-12-01

    Full Text Available Tension (TW and opposite wood (OW of Eucalyptus globulus trees were analyzed for its chemical characteristics and Kraft pulp production. Lignin content was 16% lower and contained 32% more syringyl units in TW than in OW. The increase in syringyl units favoured the formation of β-O-4 bonds that was also higher in TW than in OW (84% vs. 64%, respectively. The effect of these wood features was evaluated in the production of Kraft pulps from both types of wood. At kappa number 16, Kraft pulps obtained from TW demanded less active alkali in delignification and presented slightly higher or similar pulp yield than pulps made with OW. Fiber length, coarseness and intrinsic viscosity were also higher in tension than in opposite pulps. When pulps where refined to 30°SR, TW pulps needed 18% more revolutions in the PFI mill to achieve the same beating degree than OW pulps. Strength properties (tensile, tear and burst indexes were slightly higher or similar in tension as compared with opposite wood pulps. After an OD0(EOD1 bleaching sequence, both pulps achieved up to 89% ISO brightness. Bleached pulps from TW presented higher viscosity and low amount of hexenuronic acids than pulps from OW. Results showed that TW presented high xylans and low lignin content that caused a decrease in alkali consumption, increase pulp strength properties and similar bleaching performance as compared with pulps from OW.Madeira de tração e oposta de árvores de Eucalyptus globulus foram analisadas quanto a suas características químicas e produção de polpa Kraft. A caracterização química da madeira de tração (TW de Eucalyptus globulus Labill. mostrou um conteúdo similar de celulose, alto conteúdo de xilanas e baixo conteúdo de lignina quando comparada com a madeira oposta (OW de uma mesma árvore. O conteúdo de lignina foi 16% menor e contém 32% mais unidades siringila em TW que em OW. O aumento das unidades siringila favoreceu a formação de ligações

  2. Studies on the antidandruff activity of the essential oil of coleus amboinicus and eucalyptus globulus

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    P.Selvakumar

    2012-10-01

    Full Text Available Objective: To determine the effect of the essential oil of Coleus amboinicus and Eucalyptus globules as potential antidandruff agent. Methods: Fresh leaves of Coleus amboinicus and Eucalyptus globules were collected from the Horticultural Research Station, Yercaud, Salem, Tamil Nadu, India. The collected leaves were washed thoroughly and shade dried for 7-10 days. The essential oils from both the dried plant leaves were obtained by hydro distillation procedure. The chemical compositions of hydro distilled essential oils were analyzed by Gas Chromatography-Mass spectrometry (GC-MS. The pure essential oils and the essential oils diluted with coconut oil of the two plants were used to check antidandruff activity against the dandruff causing fungus Malassezia furfur by agar well diffusion assay. Results: The average yield of essential oil from Coleus amboinicus (0.89% and Eucalyptus globules (0.77%. The results of GC-MS revealed that the presence of eight compounds from Coleus amboinicus essential oil and five constituents from Eucalyptus globules representing 90.45% and 96.32% of the total oil respectively. The major component was identified as Thymol (phenolic rich compocnd (41.3% in Coleus amboinicus and in Eucalyptus globules the major constituents was 1-8, Cineol (91.61%. In the antidandruff activity the broad range of inhibitory zone was observed in Eucalyptus globules (37mm oil compare to Coleus amboinicus (31mm. Ketaconazole based shampoo used as a standard. Conclusion: This study would direct to the establishment of a natural compound from Coleus amboinicus and Eucalyptus globules as an antidandruff agent which can be used for the production of potential antifungal drug and novel pharmaceutical and cosmeceutical leads.

  3. Atividade ovicida e larvicida in vitro do óleo essencial de Eucalyptus globulus sobre Haemonchus contortus Ovicidal and larvicidal activity in vitro of Eucalyptus globulus essential oils on Haemonchus contortus

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    Iara T. F. Macedo

    2009-09-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito do óleo essencial de Eucalyptus globulus (OEEG sobre a eclosão e desenvolvimento de larvas de Haemonchus contortus. A determinação da composição química do OEEG foi feita por meio de cromatografia gasosa acoplada à espectrometria de massa. No teste de eclosão de ovos (TEO, utilizaram-se as concentrações 21,75; 17,4; 8,7; 5,43 e 2,71 mg.mL-1; e no de desenvolvimento larvar (TDL as concentrações foram 43,5; 21,75; 10,87; 5,43 e 2,71 mg.mL-1. Cada ensaio foi acompanhado por um controle positivo, 0,02 mg.mL-1 tiabendazol, no TEO e 0,008 mg.mL-1 ivermectina no TDL, e por um controle negativo com o diluente Tween 80 (3%. A eficácia máxima obtida pelo OEEG sobre ovos foi de 99,3% na concentração de 21,75 mg.mL-1 e, sobre larvas, 98,7% na concentração de 43,5 mg.mL-1. A concentração do OEEG, que inviabilizou 50% dos ovos e das larvas do parasito, foi de 8,3 e 6,92 mg.mL-1, respectivamente. A análise química do óleo identificou como principal componente o monoterpeno 1,8-cineol. O OEEG apresentou atividade ovicida e larvicida in vitro sobre H. contortus, determinando um bom potencial para utilização no controle de nematóides gastrintestinais de ovinos e caprinos.The objective of this work was to evaluate ovicidal and larvicidal effects of Eucalyptus globulus essential oil (EGEO on Haemonchus contortus. The chemical composition determination of EGEO was through gas chromatography and mass spectrometry. Egg hatch test (EHT was performed in concentrations 21.75; 17.4; 8.7; 5.43 e 2.71 mg.mL-1. In larval development test (LDT were used the concentrations 43.5; 21.75; 10.87; 5.43 e 2.71 mg.mL-1. Each trial was conducted by negative control with Tween 80 (3% and positive control, 0.02 mg.mL -1 of thiabendazole in EHT and 0.008 mg.mL-1 of ivermectin in LDT. The maximum effectiveness of EGEO on eggs was 99.3% in concentration of 21.75 mg.mL-1 and on larvae was 98.7% in concentration 43

  4. Should Exotic Eucalyptus be Planted in Subtropical China: Insights from Understory Plant Diversity in Two Contrasting Eucalyptus Chronosequences.

    Science.gov (United States)

    Wu, Jianping; Fan, Houbao; Liu, Wenfei; Huang, Guomin; Tang, Jianfu; Zeng, Ruijin; Huang, Jing; Liu, Zhanfeng

    2015-11-01

    Although Eucalyptus is widely planted in South China, whose effects on native biodiversity are unclear. The objective of this study was to quantify the richness and composition of understory plants in two contrasting Eucalyptus chronosequences in South China. One was in Zhangzhou City with plantation age of 2, 4, and 6 years after clear-cutting Chinese fir forests, while the other was in Heshan City with plantation age of 2, 3, and 24 years that reforested on barren lands. Results showed that the richness of understory plants and functional groups was not significantly altered in the Zhangzhou chronosequence, while increased in the 24-year-old plantations, with a significantly larger proportion of woody plants than the younger plantations for the Heshan chronosequence. Moreover, a higher richness of woody plants accompanied by a lower richness of herbaceous species was detected in the Zhangzhou chronosequence compared with the Heshan one. To balance the need for pulp production and plant diversity conservation, we suggest that intercropping approaches between exotic Eucalyptus plantations and native forests should be considered in the fast rotation Eucalyptus plantations. However, Eucalyptus plantations may be used as pioneer species to sustain ecosystem functioning for the degraded lands.

  5. In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.Efeito in vitro do óleo essencial de Eucalyptus globulus sobre o crescimento e desenvolvimento de Penicillium sp.

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    Marcia Holanda Nozaki

    2011-08-01

    Full Text Available Penicillium sp. is the casual agent of moulds, considered the main citrus post-harvest disease, causing loss on the quality and quantity of marketable fruits. Besides economic loss, it can be included costs, transportation and fruit storage problems. The present work had the objective to verify the fungitoxic action of Eucalyptus globules essential oil on the in vitro control of Penicillium sp., evaluating mycelial growth, production and spores germination. The experiment was conduced on the Microbiology laboratory of PUC, Toledo campus, with eight treatments: 1%, 0,5%, 0,25%, 0,1%, 0,05% e 0,025% concentrations of eucalyptus oil added to PDA media (potato-dextrose-agar, negative control (PDA with addition of 40mg of azoxystrobin/L and positive control (PDA media only. The experimental design was according to a completely randomized design, with five replicates each treatment. The eucalyptus essential oil inhibited significantly the growth in the 1%, 0,5% e 0,25% concentrations, not differing statistically from control with fungicide azoxystrobin. The treatments presented the same behavior for spores production. For spores germination, the 1% e 0,5% concentrations had better control than treatment with azoxystrobin and the concentration of 0,25%. Others oil concentrations did not present significative results on the evaluations, being statistically equal to positive control (PDA.O fungo Penicillium sp. é o agente causal dos bolores, considerada a principal doença pós-colheita em citros, levando à perdas na qualidade e quantidade dos frutos comercializáveis. Além de perdas econômicas, uma vez que nas perdas pós-colheitas estão inclusos os custos, transporte e armazenagem de frutos. O presente trabalho teve por objetivo verificar a ação fungitóxica do óleo essencial de Eucalyptus globulus no controle in vitro de Penicillium sp., avaliando seu crescimento micelial, produção e a germinação de esporos. O experimento foi conduzido no

  6. [Ovicidal and larvicidal activity in vitro of Eucalyptus globulus essential oils on Haemonchus contortus].

    Science.gov (United States)

    Macedo, Iara T F; Bevilaqua, Claudia M L; de Oliveira, Lorena M B; Camurça-Vasconcelos, Ana L F; Vieira, Luiz da S; Oliveira, Fabrício R; Queiroz-Junior, Eudson M; Portela, Bruno G; Barros, Renata S; Chagas, Ana C S

    2009-01-01

    The objective of this work was to evaluate ovicidal and larvicidal effects of Eucalyptus globulus essential oil (EGEO) on Haemonchus contortus. The chemical composition determination of EGEO was through gas chromatography and mass spectrometry. Egg hatch test (EHT) was performed in concentrations 21.75; 17.4; 8.7; 5.43 e 2.71 mg x mL(-1). In larval development test (LDT) were used the concentrations 43.5; 21.75; 10.87; 5.43 e 2.71 mg x mL(-1). Each trial was conducted by negative control with Tween 80 (3%) and positive control, 0.02 mg x mL(-1) of thiabendazole in EHT and 0.008 mg x mL(-1) of ivermectin in LDT. The maximum effectiveness of EGEO on eggs was 99.3% in concentration of 21.75 mg x mL(-1) and on larvae was 98.7% in concentration 43.5 mg x mL(-1). The concentration of EGEO that inhibits 50% of the eggs and larvae was 8.3 and 6.92 mg x mL(-1), respectively. The oil chemical analysis identified as main component the monoterpen 1,8-cineol. EGEO presented ovicidal and larvicidal activities in vitro, revealing a good potential for use in the control of sheep and goat gastrointestinal nematodes.

  7. Nickel-tolerant ectomycorrhizal Pisolithus albus ultramafic ecotype isolated from nickel mines in New Caledonia strongly enhance growth of the host plant Eucalyptus globulus at toxic nickel concentrations.

    Science.gov (United States)

    Jourand, Philippe; Ducousso, Marc; Reid, Robert; Majorel, Clarisse; Richert, Clément; Riss, Jennifer; Lebrun, Michel

    2010-10-01

    Ectomycorrhizal (ECM) Pisolithus albus (Cooke & Massee), belonging to the ultramafic ecotype isolated in nickel-rich serpentine soils from New Caledonia (a tropical hotspot of biodiversity) and showing in vitro adaptive nickel tolerance, were inoculated to Eucalyptus globulus Labill used as a Myrtaceae plant-host model to study ectomycorrhizal symbiosis. Plants were then exposed to a nickel (Ni) dose-response experiment with increased Ni treatments up to 60 mg kg( - )(1) soil as extractable Ni content in serpentine soils. Results showed that plants inoculated with ultramafic ECM P. albus were able to tolerate high and toxic concentrations of Ni (up to 60 μg g( - )(1)) while uninoculated controls were not. At the highest Ni concentration tested, root growth was more than 20-fold higher and shoot growth more than 30-fold higher in ECM plants compared with control plants. The improved growth in ECM plants was associated with a 2.4-fold reduction in root Ni concentration but a massive 60-fold reduction in transfer of Ni from root to shoots. In vitro, P. albus strains could withstand high Ni concentrations but accumulated very little Ni in its tissue. The lower Ni uptake by mycorrhizal plants could not be explained by increased release of metal-complexing chelates since these were 5- to 12-fold lower in mycorrhizal plants at high Ni concentrations. It is proposed that the fungal sheath covering the plant roots acts as an effective barrier to limit transfer of Ni from soil into the root tissue. The degree of tolerance conferred by the ultramafic P. albus isolates to growth of the host tree species is considerably greater than previously reported for other ECM. The primary mechanisms underlying this improved growth were identified as reduced Ni uptake into the roots and markedly reduced transfer from root to shoot in mycorrhizal plants. The fact that these positive responses were observed at Ni concentrations commonly observed in serpentinic soils suggests that

  8. Efeito do teor de lignina da madeira de Eucalyptus globulus Labill. no desempenho da polpação kraft

    Directory of Open Access Journals (Sweden)

    Gabriel Valim Cardoso

    2011-01-01

    Full Text Available In this research, it was analyzed the lignin content effect of Eucalyptus globulus Labill. wood in kraft pulping optimization. Seventy-two laboratory cooking were made with wood chips obtained from six Eucalyptus globulus trees selected from a group of 50 trees. The wood chips from three trees with the lowest lignin content, with average 20.53%, were mixed proportionally based on the tree weights, obtaining the sample of low lignin content wood. The same was made to obtain the sample for wood chips with the highest lignin content, with average 23.02%. The two lignin levels were statistically different. The two wood samples had basic densities statistically not different. Using three maximum temperatures levels (160, 165 and 170ºC, and three active alkali charged (17, 18.5 and 20%, the wood chips were converted to kraft pulps. The pulps were then characterized to analyze the influence of the distinct treatments employed in the cooking on their properties. The effect of the cooking conditions was expressed by mathematical models in order to determine the optimum points for each of the evaluated properties. The optimization process indicated maximum temperature of 168ºC, and active alkali of 19%, for maximum kraft pulping yield to achieve kappa number 18; this result was for woods with low lignin content. For woods containing the high lignin content, the optimization showed maximum cooking temperature of 169ºC and active alkali of 19% for kappa number of 18. The average reduction of 2.49% in wood lignin content resulted a correspondent gain of 2.2% in the kraft yield (o.d. basis and a reduction on the active alkali charge of 1.2% (o.d. basis to achieve kappa numbers from 16 to 19, preserving pulp properties. If the option is to work with kappa number 19 instead of 16, the gain in kraft yield is approximately 2%. Therefore, when working with low lignin content wood and kappa number 19 instead of 16, a substantial gain of approximately 4.2% is

  9. Functional relationships between wood structure and vulnerability to xylem cavitation in races of Eucalyptus globulus differing in wood density.

    Science.gov (United States)

    Barotto, Antonio José; Monteoliva, Silvia; Gyenge, Javier; Martinez-Meier, Alejandro; Fernandez, María Elena

    2018-02-01

    Wood density can be considered as a measure of the internal wood structure, and it is usually used as a proxy measure of other mechanical and functional traits. Eucalyptus is one of the most important commercial forestry genera worldwide, but the relationship between wood density and vulnerability to cavitation in this genus has been little studied. The analysis is hampered by, among other things, its anatomical complexity, so it becomes necessary to address more complex techniques and analyses to elucidate the way in which the different anatomical elements are functionally integrated. In this study, vulnerability to cavitation in two races of Eucalyptus globulus Labill. with different wood density was evaluated through Path analysis, a multivariate method that allows evaluation of descriptive models of causal relationship between variables. A model relating anatomical variables with wood properties and functional parameters was proposed and tested. We found significant differences in wood basic density and vulnerability to cavitation between races. The main exogenous variables predicting vulnerability to cavitation were vessel hydraulic diameter and fibre wall fraction. Fibre wall fraction showed a direct impact on wood basic density and the slope of vulnerability curve, and an indirect and negative effect over the pressure imposing 50% of conductivity loss (P50) through them. Hydraulic diameter showed a direct negative effect on P50, but an indirect and positive influence over this variable through wood density on one hand, and through maximum hydraulic conductivity (ks max) and slope on the other. Our results highlight the complexity of the relationship between xylem efficiency and safety in species with solitary vessels such as Eucalyptus spp., with no evident compromise at the intraspecific level. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  10. Resistance to high level of Cu (Copper) by arbuscular mycorrhizal, saprobe Fungi and Eucalyptus globules

    International Nuclear Information System (INIS)

    Arriagada, C.; Pereira, G.; Machuca, A.; Alvear, M.; Martin, J.; Ocampo, J.

    2009-01-01

    The effects of saprobe and arbuscular mycorrhizal (AM) fungi on growth, chorophyll, root length colonization and succinate dehydrogenase (SDH) activity was measured in Eucalyptus globulus Labill., plants growing in soil with high level of Cu were investigated. The application of Cu inhibited the development of mycelia of the saprobe fungi Fusarium concolor and Trichoderma koningii and the hyphal length of the arbuscular mycorrhizal fungi (AM) Glomus mosseae and G. deserticola in vitro. (Author)

  11. Resistance to high level of Cu (Copper) by arbuscular mycorrhizal, saprobe Fungi and Eucalyptus globules

    Energy Technology Data Exchange (ETDEWEB)

    Arriagada, C.; Pereira, G.; Machuca, A.; Alvear, M.; Martin, J.; Ocampo, J.

    2009-07-01

    The effects of saprobe and arbuscular mycorrhizal (AM) fungi on growth, chorophyll, root length colonization and succinate dehydrogenase (SDH) activity was measured in Eucalyptus globulus Labill., plants growing in soil with high level of Cu were investigated. The application of Cu inhibited the development of mycelia of the saprobe fungi Fusarium concolor and Trichoderma koningii and the hyphal length of the arbuscular mycorrhizal fungi (AM) Glomus mosseaae and G. deserticola in vitro. (Author)

  12. Above-ground biomass production and allometric relations of Eucalyptus globulus Labill. coppice plantations along a chronosequence in the central highlands of Ethiopia

    International Nuclear Information System (INIS)

    Zewdie, Mulugeta; Olsson, Mats; Verwijst, Theo

    2009-01-01

    Eucalyptus plantations are extensively managed for wood production in the central highlands of Ethiopia. Nevertheless, little is known about their biomass (dry matter) production, partitioning and dynamics over time. Data from 10 different Eucalyptus globulus stands, with a plantation age ranging from 11 to 60 years and with a coppice-shoot age ranging from 1 to 9 years were collected and analyzed. Above-ground tree biomass of 7-10 sampled trees per stand was determined destructively. Dry weights of tree components (W c ; leaves, twigs, branches, stembark, and stemwood) and total above-ground biomass (W a ) were estimated as a function of diameter above stump (D), tree height (H) and a combination of these. The best fits were obtained, using combinations of D and H. When only one explanatory variable was used, D performed better than H. Total above-ground biomass was linearly related to coppice-shoot age. In contrast a negative relation was observed between the above-ground biomass production and total plantation age (number of cutting cycles). Total above-ground biomass increased from 11 t ha -1 at a stand age of 1 year to 153 t ha -1 at 9 years. The highest dry weight was allocated to stemwood and decreased in the following order: stemwood > leaves > stembark > twigs > branches. The equations developed in this study to estimate biomass components can be applied to other Eucalyptus plantations under the assumption that the populations being studied are similar with regard to density and tree size to those for which the relationships were developed

  13. Above-ground biomass production and allometric relations of Eucalyptus globulus Labill. coppice plantations along a chronosequence in the central highlands of Ethiopia

    Energy Technology Data Exchange (ETDEWEB)

    Zewdie, Mulugeta; Olsson, Mats; Verwijst, Theo [Swedish University of Agricultural Sciences, Department of Crop Production Ecology, P.O. Box 7043, 75007 Uppsala (Sweden)

    2009-03-15

    Eucalyptus plantations are extensively managed for wood production in the central highlands of Ethiopia. Nevertheless, little is known about their biomass (dry matter) production, partitioning and dynamics over time. Data from 10 different Eucalyptus globulus stands, with a plantation age ranging from 11 to 60 years and with a coppice-shoot age ranging from 1 to 9 years were collected and analyzed. Above-ground tree biomass of 7-10 sampled trees per stand was determined destructively. Dry weights of tree components (W{sub c}; leaves, twigs, branches, stembark, and stemwood) and total above-ground biomass (W{sub a}) were estimated as a function of diameter above stump (D), tree height (H) and a combination of these. The best fits were obtained, using combinations of D and H. When only one explanatory variable was used, D performed better than H. Total above-ground biomass was linearly related to coppice-shoot age. In contrast a negative relation was observed between the above-ground biomass production and total plantation age (number of cutting cycles). Total above-ground biomass increased from 11 t ha{sup -1} at a stand age of 1 year to 153 t ha{sup -1} at 9 years. The highest dry weight was allocated to stemwood and decreased in the following order: stemwood > leaves > stembark > twigs > branches. The equations developed in this study to estimate biomass components can be applied to other Eucalyptus plantations under the assumption that the populations being studied are similar with regard to density and tree size to those for which the relationships were developed. (author)

  14. Fungi associated to bark lesions of Eucalyptus globulus stems in plantations from Uruguay Fungos asociados as lesões da casca do caule de Eucalyptus globulus em plantações no uruguai

    Directory of Open Access Journals (Sweden)

    Raquel Alonso

    2009-08-01

    Full Text Available Trees with stem bark lesions are frequently observed in Eucalyptus globulus Labill. plantations, particularly in the central west region of Uruguay. These lesions constitute a problem for trunk decortications at harvest and they also facilitate the access of fungi that could cause wood decay. Seven, three and oneyear-old plantations, located at three sites in close proximity were selected. Four types of trunk lesions were present in trees regardless the age of plantation and more than one type was found in each plantation. The aim of this study was to investigate the fungal composition associated with these lesions and compare them to healthy tissues and try to find out the origin of these symptoms. Another purpose was to elucidate the real role of the fungi considered pathogens by means of experimental inoculations. Segments from lesions and healthy tissues yielded 897 fungal isolates belonging to 32 taxa, 681 isolates from bark lesions and 216 from healthy tissues. Both healthy and symptomatic tissues showed similar fungal species composition, but with differences in frequencies of colonization. Cytospora eucalypticola Van der Westhuizen, Botryosphaeria spp., Pestalotiopsis guepinii (Desm. Stey. and Penicillium spp. were the dominant species isolated. As symptoms were not reproduced after experimental inoculation with Botryosphaeria ribis Grossenb. & Duggar and B. eucalyptorum Crous, & M.J. Wingf, it could be suggested that these lesions were originated by unfavorable environmental conditions. The frost that occurred for several days out of season and flooding may have been involved in the development of bark lesion.As lesões na casca de Eucalyptus globulus Labill. são frequentemente observadas nas plantações da Região Centro-Oeste do Uruguai. Constituem problema para o descortiçamento na colheita e, além disso, facilita a penetraçao de fungos apodrecedores da madeira. Selecionaram-se plantações com 1, 3 e 7 anos de idade, em tr

  15. Cambios anatómicos y morfológicos en plántulas de Eucalyptus sp. en respuesta a diferentes niveles de luz Anatomical and morphological changes in Eucalyptus sp. seedlings in response to different levels of light

    Directory of Open Access Journals (Sweden)

    Ana B. Guarnaschelli

    2009-12-01

    Full Text Available Se evaluaron respuestas en la anatomía y morfología de plántulas de Eucalyptus globulus subesp. globulus, Eucalyptus grandis y Eucalyptus camaldulensis creciendo bajo diferentes niveles de luz. Las plantas fueron expuestas a tres regímenes de iluminación: pleno sol (C, 50 % (S50 y 75% de restricción lumínica (S75 y regadas diariamente. Se midieron características morfológicas y anatómicas del follaje, se calcularon coeficientes foliares e índices de plasticidad. Al disminuir la disponibilidad lumínica, las plantas mostraron cambios asociados a la aclimatación a la sombra. Se observaron aumentos en el área foliar y en los coeficientes foliares. Paralelamente se detectaron disminuciones en el espesor de las hojas, de la epidermis, del parénquima en empalizada y en la densidad de estomas. La biomasa foliar y la biomasa total diminuyeron significativamente bajo el nivel S75. El aumento en el nivel de restricción lumínica produjo cambios proporcionales en el espesor de hojas y en el coeficiente de área foliar. Independientemente del nivel de sombra, se detectaron diferencias entre especies, que podrían asociarse a las condiciones ambientales de sus áreas de origen. Si bien se observaron algunas diferencias en los índices de plasticidad, los valores promedio de las tres especies fueron similares. Esto sugiere que, para los caracteres considerados en este estudio, el grado de aclimatación a la sombra fue similar.Responses in the anatomy and morphology of Eucalyptus globulus subsp. globulus, Eucalyptus grandis and Eucalyptus camaldulensis seedlings, growing under different levels of light were evaluated. Seedlings were submitted to three levels of irradiance: full sun (C, 50% (S50 and 75% of light restriction (S75 and irrigated daily. Morphological and anatomical characteristics were measured; foliar coefficients and indexes of plasticity were calculated. As light availability diminished, plants showed changes associated with shade

  16. Supplementary data: Eucalyptus microsatellites mined in silico ...

    Indian Academy of Sciences (India)

    Supplementary data: Eucalyptus microsatellites mined in silico: survey and evaluation. R. Yasodha, R. Sumathi, P. Chezhian, S. Kavitha and M. Ghosh. J. Genet. 87, XX-XX. Tm. CT. 2222. NA. 60 125. 192. Table 1. List of EST-SSR primers developed for E. globulus. No. of. Tm Product. Acc. no. SSR repeats. Forward primer.

  17. Inhibition of growth and biofilm formation of clinical bacterial isolates by NiO nanoparticles synthesized from Eucalyptus globulus plants.

    Science.gov (United States)

    Saleem, Samia; Ahmed, Bilal; Khan, Mohammad Saghir; Al-Shaeri, Majed; Musarrat, Javed

    2017-10-01

    Nanotechnology based therapeutics has emerged as a promising approach for augmenting the activity of existing antimicrobials due to the unique physical and chemical properties of nanoparticles (NPs). Nickel oxide nanoparticles (NiO-NPs) have been suggested as prospective antibacterial and antitumor agent. In this study, NiO-NPs have been synthesized by a green approach using Eucalyptus globulus leaf extract and assessed for their bactericidal activity. The morphology and purity of synthesized NiO-NPs determined through various spectroscopic techniques like UV-Visible, FT-IR, XRD, EDX and electron microscopy differed considerably. The synthesized NiO-NPs were pleomorphic varying in size between 10 and 20 nm. The XRD analysis revealed the average size of NiO-NPs as 19 nm. The UV-Vis spectroscopic data showed a strong SPR of NiO-NPs with a characteristic spectral peak at 396 nm. The FTIR data revealed various functional moieties like C=C, C-N, C-H and O-H which elucidate the role of leaf biomolecules in capping and dispersal of NiO-NPs. The bioactivity assay revealed the antibacterial and anti-biofilm activity of NiO-NPs against ESβL (+) E. coli, P. aeruginosa, methicillin sensitive and resistant S. aureus. Growth inhibition assay demonstrated time and NiO-NPs concentration dependent decrease in the viability of treated cells. NiO-NPs induced biofilm inhibition was revealed by a sharp increase in characteristic red fluorescence of PI, while SEM images of NiO-NPs treated cells were irregular shrink and distorted with obvious depressions/indentations. The results suggested significant antibacterial and antibiofilm activity of NiO-NPs which may play an important role in the management of infectious diseases affecting human health. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. The effect of vapour pressure deficit on stomatal conductance, sap pH and leaf-specific hydraulic conductance in Eucalyptus globulus clones grown under two watering regimes.

    Science.gov (United States)

    Hernandez, Maria Jose; Montes, Fernando; Ruiz, Federico; Lopez, Gustavo; Pita, Pilar

    2016-05-01

    Stomatal conductance has long been considered of key interest in the study of plant adaptation to water stress. The expected increase in extreme meteorological events under a climate change scenario may compromise survival in Eucalyptus globulus plantations established in south-western Spain. We investigated to what extent changes in stomatal conductance in response to high vapour pressure deficits and water shortage are mediated by hydraulic and chemical signals in greenhouse-grown E. globulus clones. Rooted cuttings were grown in pots and submitted to two watering regimes. Stomatal conductance, shoot water potential, sap pH and hydraulic conductance were measured consecutively in each plant over 4 weeks under vapour pressure deficits ranging 0·42 to 2·25 kPa. Evapotranspiration, growth in leaf area and shoot biomass were also determined. There was a significant effect of both clone and watering regime in stomatal conductance and leaf-specific hydraulic conductance, but not in sap pH. Sap pH decreased as water potential and stomatal conductance decreased under increasing vapour pressure deficit. There was no significant relationship between stomatal conductance and leaf-specific hydraulic conductance. Stomata closure precluded shoot water potential from falling below -1·8 MPa. The percentage loss of hydraulic conductance ranged from 40 to 85 %. The highest and lowest leaf-specific hydraulic conductances were measured in clones from the same half-sib families. Water shortage reduced growth and evapotranspiration, decreases in evapotranspiration ranging from 14 to 32 % in the five clones tested. Changes in sap pH seemed to be a response to changes in atmospheric conditions rather than soil water in the species. Stomata closed after a considerable amount of hydraulic conductance was lost, although intraspecific differences in leaf-specific hydraulic conductance suggest the possibility of selection for improved productivity under water-limiting conditions

  19. Kraft pulping and ECF bleaching of Eucalyptus globulus pretreated by the white-rot fungus Ceriporiopsis subvermispora - doi: 10.4025/actascitechnol.v34i3.12410

    Directory of Open Access Journals (Sweden)

    Claudio Salazar

    2012-05-01

    Full Text Available Eucalyptus globulus wood chips were decayed by the lignin-degrading fungus Ceriporiopsis subvermispora as a pretreatment step before kraft pulping. Weight and component losses of wood after the biotreatment were the following: weight (5%, glucans (1.5%, xylans (4.3%, lignin (5.7% and extractives (57.5%. The residual amount of lignin (expressed by the kappa number in pulps from biotreated wood chips was lower than that of pulps from the undecayed control. Depending on the delignification degree, kraft biopulps presented similar or up to 4% increase in pulp yield and 20% less hexenuronic acids (HexA than control pulps. The extended delignification with O2 decreases approximately 50% of the kappa number of the pulps and increases brightness, but had no effect in HexA reduction. The bleaching steps with chlorine dioxide (D0ED1 sequence decreased the kappa number up to 97%, increased pulp brightness up to 84% ISO and decreased HexA amount up to 91%. The use of C. subvermispora in biopulping of E. globulus generated important benefits during the production of kraft pulps that are reflected in a high pulp yield, low residual lignin content, low HexA amount, high brightness and viscosity of the biopulps as compared with pulps produced from untreated wood chips.

  20. Wood density and anatomy of three Eucalyptus species: implications for hydraulic conductivity

    OpenAIRE

    Antonio J. Barotto; Silvia Monteoliva; Javier Gyenge; Alejandro Martínez-Meier; Karen Moreno; Natalia Tesón; María Elena Fernández

    2017-01-01

    Aim of the study: To characterize wood anatomical traits of three Eucalyptus species that differ in wood density and ecological requirements, and to examine the relationships between some anatomical features, wood density, and theoretical xylem hydraulic conductivity (Ks). Area of study: We analyzed 86 trees from three sites of Argentina (Entre Ríos and Buenos Aires Provinces). Methods: The sampled trees were Eucalyptus globulus, E. grandis and E. viminalis ranging from 11 to 15 years...

  1. Validation of reference genes from Eucalyptus spp. under different stress conditions

    Directory of Open Access Journals (Sweden)

    Moura Jullyana Cristina Magalhães Silva

    2012-11-01

    Full Text Available Abstract Background The genus Eucalyptus consists of approximately 600 species and subspecies and has a physiological plasticity that allows some species to propagate in different regions of the world. Eucalyptus is a major source of cellulose for paper manufacturing, and its cultivation is limited by weather conditions, particularly water stress and low temperatures. Gene expression studies using quantitative reverse transcription polymerase chain reaction (qPCR require reference genes, which must have stable expression to facilitate the comparison of the results from analyses using different species, tissues, and treatments. Such studies have been limited in eucalyptus. Results Eucalyptus globulus Labill, Eucalyptus urograndis (hybrid from Eucalyptus urophylla S.T. Blake X Eucalyptus grandis Hill ex-Maiden and E. uroglobulus (hybrid from E. urograndis X E. globulus were subjected to different treatments, including water deficiency and stress recovery, low temperatures, presence or absence of light, and their respective controls. Except for treatment with light, which examined the seedling hypocotyl or apical portion of the stem, the expression analyses were conducted in the apical and basal parts of the stem. To select the best pair of genes, the bioinformatics tools GeNorm and NormFinder were compared. Comprehensive analyses that did not differentiate between species, treatments, or tissue types, showed that IDH (isocitrate dehydrogenase, SAND (SAND protein, ACT (actin, and A-Tub (α-tubulin genes were the most stable. IDH was the most stable gene in all of the treatments. Conclusion Comparing these results with those of other studies on eucalyptus, we concluded that five genes are stable in different species and experimental conditions: IDH, SAND, ACT, A-Tub, and UBQ (ubiquitin. It is usually recommended a minimum of two reference genes is expression analysis; therefore, we propose that IDH and two others genes among the five identified

  2. Eucalyptus sp. at the Intersection of Environment and Culture in Kenya

    Directory of Open Access Journals (Sweden)

    Brandy M Garrett Kluthe

    2017-04-01

    Full Text Available Members of the genus Eucalyptus are popular on small farms throughout Kenya, and include species such as Eucalyptus saligna, Eucalpytus globulus, and Eucalyptus grandis. Although they are fast-growing and perform well on marginal land, these trees are associated with negative environmental effects in Africa and elsewhere they have been introduced. In-person surveys were conducted with small farmers in Kenya to determine patterns of Eucalyptus sp. stand use and cultural importance. It was found that despite acknowledged short and long term environmental consequences, Eucalyptus sp. remains popular for medium term economic investment. These findings are consistent with other reports of Eucalyptus sp. woodlots serving as funding sources for education, health emergencies, etc., in parts of the world where bank loans are unavailable. Further, it was found that some farmers are using modified silviculture practices to ameliorate the negative effects of Eucalyptus sp., and others are seeking indigenous alternatives.           

  3. Wood density and anatomy of three Eucalyptus species: implications for hydraulic conductivity

    OpenAIRE

    Barotto, Antonio J.; Monteoliva, Silvia; Gyenge, Javier; Martínez-Meier, Alejandro; Moreno, Karen; Tesón, Natalia; Fernández, María Elena

    2017-01-01

    Aim of the study: To characterize wood anatomical traits of three Eucalyptus species that differ in wood density and ecological requirements, and to examine the relationships between some anatomical features, wood density, and theoretical xylem hydraulic conductivity (Ks).Area of study: We analyzed 86 trees from three sites of Argentina (Entre Ríos and Buenos Aires Provinces).Methods: The sampled trees were Eucalyptus globulus, E. grandis and E. viminalis ranging from 11 to 15 years old. One ...

  4. Patterns of Reproductive Isolation in Eucalyptus-A Phylogenetic Perspective.

    Science.gov (United States)

    Larcombe, Matthew J; Holland, Barbara; Steane, Dorothy A; Jones, Rebecca C; Nicolle, Dean; Vaillancourt, René E; Potts, Brad M

    2015-07-01

    We assess phylogenetic patterns of hybridization in the speciose, ecologically and economically important genus Eucalyptus, in order to better understand the evolution of reproductive isolation. Eucalyptus globulus pollen was applied to 99 eucalypt species, mainly from the large commercially important subgenus, Symphyomyrtus. In the 64 species that produce seeds, hybrid compatibility was assessed at two stages, hybrid-production (at approximately 1 month) and hybrid-survival (at 9 months), and compared with phylogenies based on 8,350 genome-wide DArT (diversity arrays technology) markers. Model fitting was used to assess the relationship between compatibility and genetic distance, and whether or not the strength of incompatibility "snowballs" with divergence. There was a decline in compatibility with increasing genetic distance between species. Hybridization was common within two closely related clades (one including E. globulus), but rare between E. globulus and species in two phylogenetically distant clades. Of three alternative models tested (linear, slowdown, and snowball), we found consistent support for a snowball model, indicating that the strength of incompatibility accelerates relative to genetic distance. Although we can only speculate about the genetic basis of this pattern, it is consistent with a Dobzhansky-Muller-model prediction that incompatibilities should snowball with divergence due to negative epistasis. Different rates of compatibility decline in the hybrid-production and hybrid-survival measures suggest that early-acting postmating barriers developed first and are stronger than later-acting barriers. We estimated that complete reproductive isolation can take up to 21-31 My in Eucalyptus. Practical implications for hybrid eucalypt breeding and genetic risk assessment in Australia are discussed. © The Author 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For

  5. Drought increases heat tolerance of leaf respiration in Eucalyptus globulus saplings grown under both ambient and elevated atmospheric [CO2] and temperature

    Science.gov (United States)

    Gauthier, Paul P. G.; Crous, Kristine Y.; Ayub, Gohar; Duan, Honglang; Weerasinghe, Lasantha K.; Ellsworth, David S.; Tjoelker, Mark G.; Evans, John R.; Tissue, David T.; Atkin, Owen K.

    2014-01-01

    Climate change is resulting in increasing atmospheric [CO2], rising growth temperature (T), and greater frequency/severity of drought, with each factor having the potential to alter the respiratory metabolism of leaves. Here, the effects of elevated atmospheric [CO2], sustained warming, and drought on leaf dark respiration (R dark), and the short-term T response of R dark were examined in Eucalyptus globulus. Comparisons were made using seedlings grown under different [CO2], T, and drought treatments. Using high resolution T–response curves of R dark measured over the 15–65 °C range, it was found that elevated [CO2], elevated growth T, and drought had little effect on rates of R dark measured at T CO2], growth T, and drought on T response of R dark. However, drought increased R dark at high leaf T typical of heatwave events (35–45 °C), and increased the measuring T at which maximal rates of R dark occurred (T max) by 8 °C (from 52 °C in well-watered plants to 60 °C in drought-treated plants). Leaf starch and soluble sugars decreased under drought and elevated growth T, respectively, but no effect was found under elevated [CO2]. Elevated [CO2] increased the Q 10 of R dark (i.e. proportional rise in R dark per 10 °C) over the 15–35 °C range, while drought increased Q 10 values between 35 °C and 45 °C. Collectively, the study highlights the dynamic nature of the T dependence of R dark in plants experiencing future climate change scenarios, particularly with respect to drought and elevated [CO2]. PMID:25205579

  6. Oligo-carrageenan kappa-induced reducing redox status and activation of TRR/TRX system increase the level of indole-3-acetic acid, gibberellin A3 and trans-zeatin in Eucalyptus globulus trees.

    Science.gov (United States)

    González, Alberto; Contreras, Rodrigo A; Zúiga, Gustavo; Moenne, Alejandra

    2014-08-20

    Eucalyptus globulus trees treated with oligo-carrageenan (OC) kappa showed an increase in NADPH, ascorbate and glutathione levels and activation of the thioredoxin reductase (TRR)/thioredoxin (TRX) system which enhance photosynthesis, basal metabolism and growth. In order to analyze whether the reducing redox status and the activation of thioredoxin reductase (TRR)/thioredoxin (TRX) increased the level of growth-promoting hormones, trees were treated with water (control), with OC kappa, or with inhibitors of ascorbate synthesis, lycorine, glutathione synthesis, buthionine sulfoximine (BSO), NADPH synthesis, CHS-828, and thioredoxin reductase activity, auranofine, and with OC kappa, and cultivated for four additional months. Eucalyptus trees treated with OC kappa showed an increase in the levels of the auxin indole 3-acetic acid (IAA), gibberellin A3 (GA3) and the cytokinin trans-zeatin (t-Z) as well as a decrease in the level of the brassinosteroid epi-brassinolide (EB). In addition, treatment with lycorine, BSO, CHS-828 and auranofine inhibited the increase in IAA, GA3 and t-Z as well as the decrease in EB levels. Thus, the reducing redox status and the activation of TRR/TRX system induced by OC kappa increased the levels of IAA, GA3 and t-Z levels determining, at least in part, the stimulation of growth in Eucalyptus trees.

  7. Resource Communication. Temporal optimization of fuel treatment design in blue gum (Eucalyptus globulus) plantations

    Energy Technology Data Exchange (ETDEWEB)

    Martin, A.; Botequim, B.; Oliveira, T.M.; Ager, A.; Pirotti, F.

    2016-07-01

    Aim of the study: This study was conducted to support fire and forest management planning in eucalypt plantations based on economic, ecological and fire prevention criteria, with a focus on strategic prioritisation of fuel treatments over time. The central objective was to strategically locate fuel treatments to minimise losses from wildfire while meeting budget constraints and demands for wood supply for the pulp industry and conserving carbon. Area of study: The study area was located in Serra do Socorro (Torres Vedras, Portugal, covering ~1449 ha) of predominantly Eucalyptus globulus Labill forests managedcultivated for pulpwood by The Navigator Company. Material and methods: At each of four temporal stages (2015-2018-2021-2024) we simulated: (1) surface and canopy fuels, timber volume (m3 ha-1) and carbon storage (Mg ha-1); (2) fire behaviour characteristics, i.e. rate of spread (m min-1), and flame length (m), with FlamMap fire modelling software; (3) optimal treatment locations as determined by the Landscape Treatment Designer (LTD). Main results: The higher pressure of fire behaviour in the earlier stages of the study period triggered most of the spatial fuel treatments within eucalypt plantations in a juvenile stage. At later stages fuel treatments also included shrublands areas. The results were consistent with observations and simulation results that show high fire hazard in juvenile eucalypt stands. Research highlights: Forest management planning in commercial eucalypt plantations can potentially accomplish multiple objectives such as augmenting profits and sustaining ecological assets while reducing wildfire risk at landscape scale. However, limitations of simulation models including FlamMap and LTD are important to recognise in studies of long term wildfire management strategies. (Author)

  8. An evaluation of EDTA additions for improving the phytoremediation efficiency of different plants under various cultivation systems.

    Science.gov (United States)

    Luo, Jie; Qi, Shihua; Gu, X W Sophie; Wang, Jinji; Xie, Xianming

    2016-05-01

    Previous studies have shown that phytoremediation usually requires soil amendments, such as chelates, to mobilize low bioavailability heavy metals for better plant absorption and, consequently, for remediation efficiency. A total dry biomass of 3.39 and 0.0138 kg per plant was produced by a phytoremediator, Eucalyptus globulus, and a nitrogen fixing crop, Cicer arietinum (chickpea), respectively. The accumulation of Pb in E. globulus and chickpea reached 1170.61 and 1.33 mg per plant (700 and 324 mg kg(-1)), respectively, under an ethylene diamine tetraacetic acid (EDTA) treatment, which was a five and sixfold increase over the value in untreated experiments, respectively. EDTA enhanced the phytoremediation efficiency and increased the heavy metal concentration in the soil solution. In pot experiments, approximately 27 % of the initial Pb leached from the spiked soil after EDTA and 25 mm artificial precipitation additions into soil without plants, which was considerably larger than the value under the same conditions without EDTA application (7 %). E. globulus planted in a mixed culture had higher water use efficiency than monocultures of either species in field experiments, and E. globulus intercepted almost all of the artificial precipitation in the pot experiments. This study demonstrates that E. globulus can maximize the potential of EDTA for improving the phytoremediation efficiency and minimizing its negative effects to the environment simultaneously by absorbing the metal-rich leachate, especially in a mixed culture of E. globulus and chickpeas.

  9. Effect of the activity of the Brazilian polyherbal formulation: Eucalyptus globulus Labill, Peltodon radicans Pohl and Schinus terebinthifolius Radd in inflammatory models

    Directory of Open Access Journals (Sweden)

    Karina C.P. Medeiros

    Full Text Available The Brazilian polyherbal formulation (BPF is composed by dyes of Eucalyptus globulus Labill, Peltodon radicans Pohl and Schinus terebinthifolius Raddi in alcohol at 13.3° GL. The formulation is popularly used in Paraíba state, Brazil since 1889 and it is used as an antiseptic and anti-inflammatory medicine. The aim of this study was to evaluate the anti-inflammatory property of the polyherbal formulation. For this purpose it was used the12-O-tetradecanoylphorbol 13-acetate (TPA and capsaicin-induced mouse ear edema and the carrageenan-induced rat paw edema. The BPF at dose of 26 mL/Kg inhibited both 12-O-tetradecanoylphorbol 13-acetate (TPA and capsaicin-induced ear edema by 49% (p < 0.05 and 24% (p < 0.01 respectively. Preliminary results on carrageenan-induced rat paw edema demonstrated that oral administration also inhibited the paw edema by approximately 29%. The results demonstrate that the Brazilian polyherbal formulation has anti-inflammatory activity and the better dose was the one used by the population.

  10. EFECTO DE LA DENSIDAD DE PLANTACIÓN EN LA RENTABILIDAD DE PLANTACIONES DE Eucalyptus globulus

    Directory of Open Access Journals (Sweden)

    Emilio Guerra-Bugueño

    2014-01-01

    Full Text Available El estudio evaluó, económicamente, la respuesta de densidades de plantación y marcos de espaciamiento de Eucalyptus globulus establecido con fines pulpables. La técnica de Montecarlo se usó para examinar el efecto de las variaciones de precios y la tasa de descuento sobre la rentabilidad. El ensayo fue realizado en el Valle Central de la IX Región de Chile. Los tratamientos fueron: 1,000 árboles·ha-1(T1; 1,428 árboles·ha-1 (T2; 1,667 árboles·ha-1 (T3 y 2,000 árboles·ha-1 (T4. El volumen de producción de cada tratamiento se midió a la edad de cosecha (10 años y posteriormente se realizó un análisis financiero, para obtener la rentabilidad (valor actual neto [VAN], valor económico del suelo [VES] y tasa interna de retorno [TIR]. Los mayores valores de VAN se obtuvieron con el T1 (US$ 330·ha-1 y T4 (US$ 322·ha-1. Los pronósticos obtenidos para el VAN de T1 mostraron una probabilidad de ocurrencia de 73 %, resultando ser la opción menos riesgosa para la inversión. Los mayores rendimientos volumétricos (T4 y T3 no propician las mayores rentabilidades, pues el incremento de la densidad de plantación aumenta los costos por unidad de planta con relación al manejo de suelos, control de malezas y fertilización.

  11. Reactivity of syringyl and guaiacyl lignin units and delignification kinetics in the kraft pulping of Eucalyptus globulus wood using Py-GC-MS/FID.

    Science.gov (United States)

    Lourenço, Ana; Gominho, Jorge; Marques, António Velez; Pereira, Helena

    2012-11-01

    Eucalyptus globulus sapwood and heartwood showed no differences in lignin content (23.0% vs. 23.7%) and composition: syringyl-lignin (17.9% vs. 18.0%) and guaiacyl-lignin (4.8% vs. 5.2%). Delignification kinetics of S- and G-units in heartwood and sapwood was investigated by Py-GC-MS/FID at 130, 150 and 170°C and modeled as double first-order reactions. Reactivity differences between S and G-units were small during the main pulping phase and the higher reactivity of S over G units was better expressed in the later pulping stage. The residual lignin composition in pulps was different from wood or from samples in the initial delignification stages, with more G and H-units. S/G ratio ranged from 3 to 4.5 when pulp residual lignin was higher than 10%, decreasing rapidly to less than 1. The S/H was initially around 20 (until 15% residual lignin), decreasing to 4 when residual lignin was about 3%. Copyright © 2012 Elsevier Ltd. All rights reserved.

  12. The genome of Eucalyptus grandis

    Energy Technology Data Exchange (ETDEWEB)

    Myburg, Alexander A.; Grattapaglia, Dario; Tuskan, Gerald A.; Hellsten, Uffe; Hayes, Richard D.; Grimwood, Jane; Jenkins, Jerry; Lindquist, Erika; Tice, Hope; Bauer, Diane; Goodstein, David M.; Dubchak, Inna; Poliakov, Alexandre; Mizrachi, Eshchar; Kullan, Anand R. K.; Hussey, Steven G.; Pinard, Desre; van der Merwe, Karen; Singh, Pooja; van Jaarsveld, Ida; Silva-Junior, Orzenil B.; Togawa, Roberto C.; Pappas, Marilia R.; Faria, Danielle A.; Sansaloni, Carolina P.; Petroli, Cesar D.; Yang, Xiaohan; Ranjan, Priya; Tschaplinski, Timothy J.; Ye, Chu-Yu; Li, Ting; Sterck, Lieven; Vanneste, Kevin; Murat, Florent; Soler, Marçal; Clemente, Hélène San; Saidi, Naijib; Cassan-Wang, Hua; Dunand, Christophe; Hefer, Charles A.; Bornberg-Bauer, Erich; Kersting, Anna R.; Vining, Kelly; Amarasinghe, Vindhya; Ranik, Martin; Naithani, Sushma; Elser, Justin; Boyd, Alexander E.; Liston, Aaron; Spatafora, Joseph W.; Dharmwardhana, Palitha; Raja, Rajani; Sullivan, Christopher; Romanel, Elisson; Alves-Ferreira, Marcio; Külheim, Carsten; Foley, William; Carocha, Victor; Paiva, Jorge; Kudrna, David; Brommonschenkel, Sergio H.; Pasquali, Giancarlo; Byrne, Margaret; Rigault, Philippe; Tibbits, Josquin; Spokevicius, Antanas; Jones, Rebecca C.; Steane, Dorothy A.; Vaillancourt, René E.; Potts, Brad M.; Joubert, Fourie; Barry, Kerrie; Pappas, Georgios J.; Strauss, Steven H.; Jaiswal, Pankaj; Grima-Pettenati, Jacqueline; Salse, Jérôme; Van de Peer, Yves; Rokhsar, Daniel S.; Schmutz, Jeremy

    2014-06-11

    Eucalypts are the world s most widely planted hardwood trees. Their broad adaptability, rich species diversity, fast growth and superior multipurpose wood, have made them a global renewable resource of fiber and energy that mitigates human pressures on natural forests. We sequenced and assembled >94% of the 640 Mbp genome of Eucalyptus grandis into its 11 chromosomes. A set of 36,376 protein coding genes were predicted revealing that 34% occur in tandem duplications, the largest proportion found thus far in any plant genome. Eucalypts also show the highest diversity of genes for plant specialized metabolism that act as chemical defence against biotic agents and provide unique pharmaceutical oils. Resequencing of a set of inbred tree genomes revealed regions of strongly conserved heterozygosity, likely hotspots of inbreeding depression. The resequenced genome of the sister species E. globulus underscored the high inter-specific genome colinearity despite substantial genome size variation in the genus. The genome of E. grandis is the first reference for the early diverging Rosid order Myrtales and is placed here basal to the Eurosids. This resource expands knowledge on the unique biology of large woody perennials and provides a powerful tool to accelerate comparative biology, breeding and biotechnology.

  13. Effiacy of citronella and eucalyptus oils against Musca domestica, Cimex lectularius and Pediculus humanus

    Directory of Open Access Journals (Sweden)

    Jehangir Khan

    2017-11-01

    Full Text Available Objective: To assess potential impacts of two indigenous plant oils: the citronella (Cymbopogon nardus and eucalyptus (Eucalyptus globulus for their insecticidal effect against Musca domestica (house fly, Cimex lectularius (bed bug and Pediculus humanus (louse. Methods: The oils of these two medicinal plants were applied separately at various concentrations (1, 2 and 3 mL/cup in cups lined with filter paper containing the target insects. Mortality was evaluated after fixed intervals (6, 12 and 24 h subsequent to the release of adult insects. Results: Results showed that both oils exhibited concentration and time dependent mortality against the tested insects. Data pertaining to present investigation clearly showed that percentage mortality owing to these botanicals against these medical pests was significantly high (98.33% at the rate of 3 mL for 24 h of exposure, followed by 2 and 1 mL concentrations with 12 and 6 h of exposure times. Conclusions: The results suggest that these plant oils possess good insecticidal properties against house fly, bed bug, and louse, and are safe to humans. Furthermore, the molecular (biochemical based study of these botanicals against diverse species of pests will be of much significance to control these pest insects.

  14. Modeling and optimization of extraction process of eucalyptus essential oil (Eucalyptus globulus

    Directory of Open Access Journals (Sweden)

    Jeancarlos Moreno

    2010-06-01

    Full Text Available In this paper we have studied the effect of the moisture from the eucalyptus leaf and the time of extraction on the yield of essential oil and the production cost. We took into account parameters such as steam temperature, pressure extraction, porous bed, steam flow and temperature of condensation. A Central Composite Rotational Design was used to evaluate optimum extraction areas and the cost that would follow, searching always to increase yields and reduce costs. According to this design, the extraction with the lowest production costs (0.57 Nuevos Soles by mL of essential oil is reached when the leaves have moisture between 25 and 30% and are extracted with times between 98 and 126 min

  15. Allelopathy in agroforestry systems: the effects of leaf extracts ofCupressus lusitanica and threeEucalyptus spp. on four Ethiopian crops

    DEFF Research Database (Denmark)

    Lisanework N.; Michelsen, Anders

    1993-01-01

    The potential allelopathic effect ofCupressus lusitanica, Eucalyptus globulus, E. camaldulensis andE. saligna on seed germination, radicle and seedling growth was investigated with four crops:Cicer arietinum (chickpea),Zea mays (maize),Pisum sativum (pea) andEragrostis tef (teff). Aqueous leaf ex...

  16. Exotic Eucalyptus leaves are preferred over tougher native species but affect the growth and survival of shredders in an Atlantic Forest stream (Brazil.

    Directory of Open Access Journals (Sweden)

    Walace P Kiffer

    Full Text Available We evaluated the effect of leaves of native and exotic tree species on the feeding activity and performance of the larvae of Triplectides gracilis, a typical caddisfly shredder in Atlantic Forest streams. Leaves of four native species that differ in chemistry and toughness (Hoffmannia dusenii, Miconia chartacea, Myrcia lineata and Styrax pohlii and the exotic Eucalyptus globulus were used to determine food preferences and rates of consumption, production of fine particulate organic matter (FPOM, growth and survival of shredders. We hypothesized that the consumption rates of leaves of Eucalyptus and their effects on the growth and survival of shredders could be predicted by leaf chemistry and toughness. The larvae preferred to feed on soft leaves (H. dusenii and M. chartacea independently of the content of nutrients (N and P and secondary compounds (total phenolics. When such leaves were absent, they preferred E. globulus and did not consume the tough leaves (M. lineata and S. pohlii. In monodietary experiments, leaf consumption and FPOM production differed among the studied leaves, and the values observed for the E. globulus treatments were intermediate between the soft and tough leaves. The larvae that fed on H. dusenii and M. chartacea grew constantly over five weeks, while those that fed on E. globulus lost biomass. Larval survival was higher on leaves of H. dusenii, M. chartacea and S. pohlii than on E. globulus and M. lineata leaves. Although E. globulus was preferred over tougher leaves, long-term consumption of leaves of the exotic species may affect the abundance of T. gracilis in the studied stream. Additionally, our results suggest that leaf toughness can be a determining factor for the behavior of shredders where low-quality leaves are abundant, as in several tropical streams.

  17. Exotic Eucalyptus leaves are preferred over tougher native species but affect the growth and survival of shredders in an Atlantic Forest stream (Brazil).

    Science.gov (United States)

    Kiffer, Walace P; Mendes, Flavio; Casotti, Cinthia G; Costa, Larissa C; Moretti, Marcelo S

    2018-01-01

    We evaluated the effect of leaves of native and exotic tree species on the feeding activity and performance of the larvae of Triplectides gracilis, a typical caddisfly shredder in Atlantic Forest streams. Leaves of four native species that differ in chemistry and toughness (Hoffmannia dusenii, Miconia chartacea, Myrcia lineata and Styrax pohlii) and the exotic Eucalyptus globulus were used to determine food preferences and rates of consumption, production of fine particulate organic matter (FPOM), growth and survival of shredders. We hypothesized that the consumption rates of leaves of Eucalyptus and their effects on the growth and survival of shredders could be predicted by leaf chemistry and toughness. The larvae preferred to feed on soft leaves (H. dusenii and M. chartacea) independently of the content of nutrients (N and P) and secondary compounds (total phenolics). When such leaves were absent, they preferred E. globulus and did not consume the tough leaves (M. lineata and S. pohlii). In monodietary experiments, leaf consumption and FPOM production differed among the studied leaves, and the values observed for the E. globulus treatments were intermediate between the soft and tough leaves. The larvae that fed on H. dusenii and M. chartacea grew constantly over five weeks, while those that fed on E. globulus lost biomass. Larval survival was higher on leaves of H. dusenii, M. chartacea and S. pohlii than on E. globulus and M. lineata leaves. Although E. globulus was preferred over tougher leaves, long-term consumption of leaves of the exotic species may affect the abundance of T. gracilis in the studied stream. Additionally, our results suggest that leaf toughness can be a determining factor for the behavior of shredders where low-quality leaves are abundant, as in several tropical streams.

  18. Modelling stand biomass fractions in Galician Eucalyptus globulus plantations by use of different LiDAR pulse densities

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez-Ferreiro, E.; Miranda, D.; Barreiro-Fernandez, L.; Bujan, S.; Garcia-Gutierrez, J.; Dieguez-Aranda, U.

    2013-07-01

    Aims of study: To evaluate the potential use of canopy height and intensity distributions, determined by airborne LiDAR, for the estimation of crown, stem and aboveground biomass fractions. To assess the effects of a reduction in LiDAR pulse densities on model precision. Area of study: The study area is located in Galicia, NW Spain. The forests are representative of Eucalyptus globulus stands in NW Spain, characterized by low-intensity silvicultural treatments and by the presence of tall shrub. Material and methods: Linear, multiplicative power and exponential models were used to establish empirical relationships between field measurements and LiDAR metrics. A random selection of LiDAR returns and a comparison of the prediction errors by LiDAR pulse density factor were performed to study a possible loss of fit in these models. Main results: Models showed similar goodness-of-fit statistics to those reported in the international literature. R2 ranged from 0.52 to 0.75 for stand crown biomass, from 0.64 to 0.87 for stand stem biomass, and from 0.63 to 0.86 for stand aboveground biomass. The RMSE/MEAN 100 of the set of fitted models ranged from 17.4% to 28.4%. Models precision was essentially maintained when 87.5% of the original point cloud was reduced, i.e. a reduction from 4 pulses m{sup 2} to 0.5 pulses m{sup 2}. Research highlights: Considering the results of this study, the low-density LiDAR data that are released by the Spanish National Geographic Institute will be an excellent source of information for reducing the cost of forest inventories. (Author)

  19. Comparative Effects of Some Medicinal Plants: Anacardium occidentale, Eucalyptus globulus, Psidium guajava, and Xylopia aethiopica Extracts in Alloxan-Induced Diabetic Male Wistar Albino Rats

    Science.gov (United States)

    Okpashi, Victor Eshu; Bayim, Bayim Peter-Robins; Obi-Abang, Margaret

    2014-01-01

    Insulin therapy and oral antidiabetic agents/drugs used in the treatment of diabetes mellitus have not sufficiently proven to control hyperlipidemia, which is commonly associated with the diabetes mellitus. Again the hopes that traditional medicine and natural plants seem to trigger researchers in this area is yet to be discovered. This research was designed to compare the biochemical effects of some medicinal plants in alloxan-induced diabetic male Wistar rats using named plants that are best at lowering blood glucose and hyperlipidemia and ameliorating other complications of diabetes mellitus by methods of combined therapy. The results obtained showed 82% decrease in blood glucose concentration after the 10th hour to the fortieth hour. There was significant increase P 0.05 recorded in the glutathione peroxidase activity of E. globulus (100 mg/kg) when compared to the test groups of P. guajava (250 mg/kg) and X. aethiopica (250 mg/kg). Catalase activity showed significant increase P 0.05, there was no significant difference seen between test group and treated groups. Meanwhile, degree of significance was observed in other parameters analysed. The biochemical analysis conducted in this study showed positive result, attesting to facts from previous works. Though these individual plants extracts exhibited significant increase in amelorating diabetes complication and blood glucose control compared to glibenclamide, a synthetic antidiabetic drug. Greater performance was observed in the synergy groups. Therefore, a poly/combined formulation of these plants extracts yielded significant result as well as resolving some other complications associated with diabetics. PMID:25525518

  20. ESTIMATIVA DA BIOMASSA E DO CONTEÚDO DE NUTRIENTES DE UM POVOAMENTO DE Eucalyptus globulus (Labillardière SUB-ESPÉCIE maidenii

    Directory of Open Access Journals (Sweden)

    Mauro Valdir Schumacher

    2000-12-01

    Full Text Available O presente trabalho teve por objetivo estimar a produção de biomassa acima do solo e determinar o conteúdo de nutrientes nos diferentes componentes das árvores de um povoamento de Eucalyptus globulus subespécie maidenii. Os dados foram coletados em um talhão de 4 anos de idade, plantado em solo argiloso, no município de Butiá, Rio Grande do Sul. Para determinar a quantidade de biomassa, foi utilizada uma equação de regressão (lnY = a + b * lnX cujos coeficientes foram calculados com base em nove árvores amostradas em diferentes classes diamétricas. A biomassa total acima do solo foi de 83,2 Mg ha-1, em que 13; 10; 9 e 68 % encontravam-se distribuídos nas folhas, ramos, casca e madeira, respectivamente. Do total dos elementos na árvore, na madeira do tronco foram encontrados 29; 29; 40; 12,5 e 34% de N, P, K, Ca e Mg respectivamente. Já na copa das árvores, esses mesmos elementos representaram 64; 56; 48,5; 32 e 39%. A casca foi o componente que acumulou as maiores quantidades de cálcio, (55,3% do total.

  1. Evaluation of the use potential of nine species of genus Eucalyptus for production of veneers and plywood panels

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2013-06-01

    Full Text Available The objective of thisstudywas to evaluate the use potential of nine species of Eucalyptus for production of veneer sheets and multilaminated plywood panels. Veneers were cut using a pilot laminating lathe to a nominal thickness of 2.0 mm. Analysis included finding values of overall yield and yield according to three quality classes for the nine relevant species. Plywood panels were manufactured in a laboratory, consisting of five 2.0 mm veneer sheets which were bonded together with phenol-formaldehyde resin at a weight of 360 g/m2 (double line. The panels were compressed using a specific pressure of 10 kgf/cm2, a temperature of 140ºC and a pressing time of 10 minutes. Results indicated that, with the exception of E. phaeotricha and E. pellita, all other Eucalyptus species had above 50% average veneer yield after lamination. Results of glue line shear testing and static bending parallel and perpendicular demonstrated that species Eucalyptus grandis, Eucalyptus saligna, Eucalyptus dunnii, Eucalyptus globulus, Eucalyptus viminalis, Eucalyptus robusta and Eucalyptus pellita have great potential within the parameters of this study for use in the production of veneer sheets and plywood panels intended for outdoor use.

  2. Effect of plant essential oils on Ralstonia solanacearum race 4 causing bacterial wilt of edible ginger

    Science.gov (United States)

    Palmarosa (Cymbopogon martini), lemongrass (C. citratus) and eucalyptus (Eucalyptus globulus) oils were investigated for their effects on Ralstonia solanacearum race 4, and their potential use as bio-fumigants for treating pathogen- infested edible ginger (Zingiber officinale R.) fields. Three conce...

  3. A Biorefinery Cascade Conversion of Hemicellulose-Free Eucalyptus Globulus Wood: Production of Concentrated Levulinic Acid Solutions for γ-Valerolactone Sustainable Preparation

    Directory of Open Access Journals (Sweden)

    Sandra Rivas

    2018-04-01

    Full Text Available Eucalyptus globulus wood samples were subjected to preliminary aqueous processing to remove water-soluble extractives and hemicelluloses, and the resulting solid (mainly made up of cellulose and lignin was employed as a substrate for converting the cellulosic fraction into mixtures of levulinic and formic acid through a sulfuric acid-catalyzed reaction. These runs were carried out in a microwave-heated reactor at different temperatures and reaction times, operating in single-batch or cross-flow modes, in order to identify the most favorable operational conditions. Selected liquid phases deriving from these experiments, which resulted in concentrated levulinic acid up to 408 mmol/L, were then employed for γ-valerolactone production by levulinc acid hydrogenation in the presence of the commercial 5% Ru/C catalyst. In order to assess the effects of the main reaction parameters, hydrogenation experiments were performed at different temperatures, reaction times, amounts of ruthenium catalyst and hydrogen pressure. Yields of γ-valerolactone in the range of 85–90 mol % were obtained from the hydrogenation of the wood-derived solutions containing levulinic acid, obtained by single-batch operation or by the cross-flow process. The negative effect of co-produced formic acid present in crude levulinic acid solutions was evidenced and counteracted efficiently by allowing the preliminary thermal decomposition of formic acid itself.

  4. Decomposição de agulhas de Pinus pinaster e de folhas de Eucalyptus globulus em regiões do interior e do litoral de Portugal Decomposition of needle litter of Pinus pinaster and leaf litter of Eucalyptus globulus in the littoral and inland areas of Portugal

    Directory of Open Access Journals (Sweden)

    C. Ribeiro

    2007-07-01

    Full Text Available Estudou-se, pela metodologia dos litter-bags, a decomposição de agulhas de Pinus pinaster (PP e de folhas de Eucalyptus globulus (EG, considerando a taxa de decomposição e a dinâmica de libertação dos nutrientes mais relevantes para a sustentabilidade dos sistemas florestais. Os estudos decorreram no litoral da Região Centro (Furadouro, Óbidos, quer com folhas de EG quer com agulhas de PP, no interior da Região Norte (Vila Pouca de Aguiar, com agulhas de PP, e numa situação intermédia com folhas de EG (Pegões e Rio Maior. Para igual período, a taxa de decomposição das agulhas de PP, estimada pelo modelo exponencial simples, foi inferior à determinada para as folhas de EG, sendo a diferença mais acentuada na fase inicial da decomposição (6 meses, em que a perda de peso das agulhas de PP foi cerca de metade da observada para as folhas de EG. Durante a fase inicial decomposição, tanto das folhas de EG como das agulhas de PP, ocorreu uma assinalável libertação de P, K e de Mg. A libertação do N dependeu da taxa de decomposição, observando- -se imobilização para as agulhas de PP com mais baixa taxa de decomposição, e libertação rápida para as folhas de EG com mais elevada taxa de decomposição. No caso do Ca o factor diferenciador foi a espécie, sendo a respectiva libertação baixa para as agulhas de PP, mas acentuada para as folhas de EG. As folhas verdes de resíduos de abate de EG decompuseram-se e libertaram os nutrientes mais rapidamente do que as folhas senescentes da mesma espécie.Decomposition of needle litter of Pinus pinaster (PP and leaf litter of Eucalyptus globulus (EG was assessed using the litterbag methodology. Decomposition rate of these residues and release dynamics of nutrients with more relevance to the sustainability of forest systems were measured. The study was carried out in the litoral of Central Portugal (Furadouro,Óbidos, using both PP and EG, in inland of North Portugal (Vila Pouca

  5. Bioenergy for District Bioheating System (DBS) from eucalyptus residues in a European coal-producing region

    International Nuclear Information System (INIS)

    Paredes-Sánchez, José P.; López-Ochoa, Luis M.; López-González, Luis M.; Xiberta-Bernat, Jorge

    2016-01-01

    Highlights: • The paper introduces a combined method to evaluate bioenergy. • Forest biomass needs to be studied as a fuel supplier and carbon sink. • The forests under study produce about 28 kt dry and 0.15 Mt CO 2 per year. • Examined a District Bioheating System (DBS) with the available biomass. - Abstract: Since forest biomass can substitute for CO 2 -emitting fossil fuels in the energy sector, forest management can greatly affect the global carbon cycle. Eucalyptus globulus has adapted very well in the coal region of the Principality of Asturias (Northwestern Spain) and has become highly regarded as a valuable raw material for the pulp and paper industry. In the present work, the Eucalyptus globulus is studied as a key natural energy source in order to improve existing methods and develop new ways of optimizing the evaluation and use of both forest biomass and woody residue in energy systems, in accordance with sustainable forestry industry safety and environmental requirements. The feasibility of utilizing forest biomass instead of natural gas in a District Bioheating System (DBS) has been examined based on an analysis of its economical and environmental impacts.

  6. Analysis of Naturally Occurring Phenolic Compounds in Aromatic Plants by RP-HPLC Coupled to Diode Array Detector (DAD and GC-MS after Silylation

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    Charalampos Proestos

    2013-03-01

    Full Text Available The following aromatic plants of Greek origin, Origanum dictamnus (dictamus, Eucalyptus globulus (eucalyptus, Origanum vulgare L. (oregano, Mellisa officinalis L. (balm mint and Sideritis cretica (mountain tea, were examined for the content of phenolic substances. Reversed phase HPLC coupled to diode array detector (DAD was used for the analysis of the plant extracts. The gas chromatography-mass spectrometry method (GC-MS was also used for identification of phenolic compounds after silylation. The most abundant phenolic acids were: gallic acid (1.5–2.6 mg/100 g dry sample, ferulic acid (0.34–6.9 mg/100 g dry sample and caffeic acid (1.0–13.8 mg/100 g dry sample. (+-Catechin and (−-epicatechin were the main flavonoids identified in oregano and mountain tea. Quercetin was detected only in eucalyptus and mountain tea.

  7. Comparative transcriptional analysis provides new insights into the molecular basis of adventitious rooting recalcitrance in Eucalyptus.

    Science.gov (United States)

    de Almeida, Márcia Rodrigues; de Bastiani, Daniela; Gaeta, Marcos Letaif; de Araújo Mariath, Jorge Ernesto; de Costa, Fernanda; Retallick, Jeffrey; Nolan, Lana; Tai, Helen H; Strömvik, Martina V; Fett-Neto, Arthur Germano

    2015-10-01

    Adventitious rooting (AR) is essential in clonal propagation. Eucalyptus globulus is relevant for the cellulose industry due to its low lignin content. However, several useful clones are recalcitrant to AR, often requiring exogenous auxin, adding cost to clonal garden operations. In contrast, E. grandis is an easy-to-root species widely used in clonal forestry. Aiming at contributing to the elucidation of recalcitrance causes in E. globulus, we conducted a comparative analysis with these two species differing in rooting competence, combining gene expression and anatomical techniques. Recalcitrance in E. globulus is reversed by exposure to exogenous indole-3-acetic acid (IAA), which promotes important gene expression modifications in both species. The endogenous content of IAA was significantly higher in E. grandis than in E. globulus. The cambium zone was identified as an active area during AR, concentrating the first cell divisions. Immunolocalization assay showed auxin accumulation in cambium cells, further indicating the importance of this region for rooting. We then performed a cambium zone-specific gene expression analysis during AR using laser microdissection. The results indicated that the auxin-related genes TOPLESS and IAA12/BODENLOS and the cytokinin-related gene ARR1may act as negative regulators of AR, possibly contributing to the hard-to-root phenotype of E. globulus. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  8. A novel genome-wide microsatellite resource for species of Eucalyptus with linkage-to-physical correspondence on the reference genome sequence.

    Science.gov (United States)

    Grattapaglia, Dario; Mamani, Eva M C; Silva-Junior, Orzenil B; Faria, Danielle A

    2015-03-01

    Keystone species in their native ranges, eucalypts, are ecologically and genetically very diverse, growing naturally along extensive latitudinal and altitudinal ranges and variable environments. Besides their ecological importance, eucalypts are also the most widely planted trees for sustainable forestry in the world. We report the development of a novel collection of 535 microsatellites for species of Eucalyptus, 494 designed from ESTs and 41 from genomic libraries. A selected subset of 223 was evaluated for individual identification, parentage testing, and ancestral information content in the two most extensively studied species, Eucalyptus grandis and Eucalyptus globulus. Microsatellites showed high transferability and overlapping allele size range, suggesting they have arisen still in their common ancestor and confirming the extensive genome conservation between these two species. A consensus linkage map with 437 microsatellites, the most comprehensive microsatellite-only genetic map for Eucalyptus, was built by assembling segregation data from three mapping populations and anchored to the Eucalyptus genome. An overall colinearity between recombination-based and physical positioning of 84% of the mapped microsatellites was observed, with some ordering discrepancies and sporadic locus duplications, consistent with the recently described whole genome duplication events in Eucalyptus. The linkage map covered 95.2% of the 605.8-Mbp assembled genome sequence, placing one microsatellite every 1.55 Mbp on average, and an overall estimate of physical to recombination distance of 618 kbp/cM. The genetic parameters estimates together with linkage and physical position data for this large set of microsatellites should assist marker choice for genome-wide population genetics and comparative mapping in Eucalyptus. © 2014 John Wiley & Sons Ltd.

  9. EucaTool®, a cloud computing application for estimating the growth and production of Eucalyptus globulus Labill. plantations in Galicia (NW Spain

    Directory of Open Access Journals (Sweden)

    Alberto Rojo-Alboreca

    2015-12-01

    Full Text Available Aim of study: To present the software utilities and explain how to use EucaTool®, a free cloud computing application developed to estimate the growth and production of seedling and clonal blue gum (Eucalyptus globulus Labill. plantations in Galicia (NW Spain.Area of study: Galicia (NW Spain.Material and methods: EucaTool® implements a dynamic growth and production model that is valid for clonal and non-clonal blue gum plantations in the region. The model integrates transition functions for dominant height (site index curves, number of stems per hectare (mortality function and basal area, as well as output functions for tree and stand volume, biomass and carbon content.Main results: EucaTool® can be freely accessed from any device with an Internet connection, from http://app.eucatool.com. In addition, useful information about the application is published on a related website: http://www.eucatool.com.Research highlights: The application has been designed to enable forest stakeholders to estimate volume, biomass and carbon content of forest plantations from individual trees, diameter classes or stand data, as well as to estimate growth and future production (indicating the optimal rotation age for maximum income by measurement of only four stand variables: age, number of trees per hectare, dominant height and basal area.Keywords: forest management; biomass; seedling; clones; blue gum; forest tool.

  10. Essential oil composition of Eucalyptus microtheca and Eucalyptus viminalis

    OpenAIRE

    Maghsoodlou, Malek Taher; Kazemipoor, Nasrin; Valizadeh, Jafar; Falak Nezhad Seifi, Mohsen; Rahneshan, Nahid

    2015-01-01

    Objective: Eucalyptus (Fam. Myrtaceae) is a medicinal plant and various Eucalyptus species possess potent pharmacological actions against diabetes, hepatotoxicity, and inflammation. This study aims to investigate essential oil composition from leaves and flowers of E. microtheca and E. viminalis leaves growing in the Southeast of Iran. Materials and Methods: The aerial parts of these plants were collected from Zahedan, Sistan and Baluchestan province, Iran in 2013. After drying the plant mate...

  11. Monoterpene engineering in a woody plant Eucalyptus camaldulensis using a limonene synthase cDNA.

    Science.gov (United States)

    Ohara, Kazuaki; Matsunaga, Etsuko; Nanto, Kazuya; Yamamoto, Kyoko; Sasaki, Kanako; Ebinuma, Hiroyasu; Yazaki, Kazufumi

    2010-01-01

    Metabolic engineering aimed at monoterpene production has become an intensive research topic in recent years, although most studies have been limited to herbal plants including model plants such as Arabidopsis. The genus Eucalyptus includes commercially important woody plants in terms of essential oil production and the pulp industry. This study attempted to modify the production of monoterpenes, which are major components of Eucalyptus essential oil, by introducing two expression constructs containing Perilla frutescens limonene synthase (PFLS) cDNA, whose gene products were designed to be localized in either the plastid or cytosol, into Eucalyptus camaldulensis. The expression of the plastid-type and cytosol-type PFLS cDNA in transgenic E. camaldulensis was confirmed by real-time polymerase chain reaction (PCR). Gas chromatography with a flame ionization detector analyses of leaf extracts revealed that the plastidic and cytosolic expression of PFLS yielded 2.6- and 4.5-times more limonene than that accumulated in wild-type E. camaldulensis, respectively, while the ectopic expression of PFLS had only a small effect on the emission of limonene from the leaves of E. camaldulensis. Surprisingly, the high level of PFLS in Eucalyptus was accompanied by a synergistic increase in the production of 1,8-cineole and alpha-pinene, two major components of Eucalyptus monoterpenes. This genetic engineering of monoterpenes demonstrated a new potential for molecular breeding in woody plants.

  12. Microwave Accelerated Green Synthesis of Stable Silver Nanoparticles with Eucalyptus globulus Leaf Extract and Their Antibacterial and Antibiofilm Activity on Clinical Isolates.

    Directory of Open Access Journals (Sweden)

    Khursheed Ali

    Full Text Available A simple and rapid microwave assisted method of green synthesis of silver nanoparticles (AgNPs was developed using aqueous leaf extract of Eucalyptus globulus(ELE, and their antibacterial and antibiofilm potential investigated. With this aim, the aqueous solutions of ELE and AgNO3(1 mM were mixed (1:4 v/v, and microwave irradiated at 2450 Mhz, for 30 sec. The instant color change of the ELE-AgNO3 mixture from pale yellow to dark brown indicated ELE-AgNPs synthesis. The intensity of peak at 428 nm in UV-Vis spectra, due to the surface plasmon resonance of AgNPs, varied with the amount of ELE, AgNO3 concentration, pH and time of incubation. The biosynthesized ELE-AgNPs were characterized by UV-visible spectroscopy, XRD, TEM, SEM-EDX, FTIR and TGA analyses. The size of ELE-AgNPs was determined to be in range of 1.9-4.3 nm and 5-25 nm, with and without microwave treatment, respectively. SEM exhibited the capping of AgNPs with the ELE constituents, and validated by FTIR analysis. The FTIR data revealed the presence of plant organic constituents and metabolites bound to ELE-AgNPs, which contributes for their stability. The antimicrobial activity of ELE-AgNPs was assessed by growth and biofilm inhibition of extended spectrum β-lactamase (ESBL producing Pseudomonas aeruginosa, Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA and methicillin-sensitive Staphylococcus aureus (MSSA clinical bacterial isolates. The results demonstrated that S. aureus were more sensitive to ELE-AgNPs than E. coli and P. aeruginosa. MRSA exhibited higher sensitive than MSSA, whereas P. aeruginosa were more sensitive than E. coli to ELE-AgNPs treatment. Also, significant (83 ± 3% and 84 ± 5% biofilm inhibition was observed in case of S. aureus and P. aeruginosa, respectively. The results elucidated environmentally friendly, economical and quick method for production of colloidal bio-functionalized ELE-AgNPs, for effectual clinical applications, as broad

  13. Research Concerning Antimicrobial Activities of Some Essential Oils Extracted from Plants

    Directory of Open Access Journals (Sweden)

    ADRIANA DALILA CRISTE

    2014-10-01

    Full Text Available The principal components of some essential oils extracted from plants have been found to have microbial activity. Depending on the concentration, the members of this class are known to be bactericide or bacteriostatic. Their action mechanism is unclear, but some studies suggest that the compounds penetrate the cell, where they interfere with cellular metabolism. The purpose of this study was to evaluate the antimicrobial activity of 5 essential oils extracted from plants on Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus and Bacillus cereus and to determinate how different amount of the used oils can influence the results of inhibition tests. These results showed that mainly all the natural extracts presented an antimicrobial effect. Thereby, some extracts were more efficient than another and the order is: Eucalyptus globulus (eucalyptus, Mentha piperita (mint, Lavandula angustifolia (lavender, Matricaria chamomilla (chamomile, Calendula officinalis (calendula.

  14. Predicción bayesiana de parámetros genéticos en clones de Eucalyptus globulus bajo condiciones de suplemento hídrico

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    Freddy Mora

    2013-01-01

    Full Text Available Se realizó un análisis Bayesiano de parámetros genéticos del crecimiento en 29 clones de Eucalyptus globulus de doce meses de edad, en el sur de Chile. Se consideraron dos condiciones ambientales contrastantes en cuanto a la disponibilidad hídrica: 1. Sin irrigación, y 2. Las plantas se irrigaron con un sistema localizado de riego. Para el análisis Bayesiano se utilizó el algoritmo de Gibbs en un modelo de interacción clonambiente. Los valores de heredabilidad fueron altos en la condición de riego (moda a posteriori de H^2=0,41, 0,36 y 0,39 para la altura, diámetro y área basal, respectivamente, en tanto que en la situación sin riego, los valores de las heredabilidades fueron significativamente más bajos; confirmado por medio de los intervalos de credibilidad Bayesianos (95 % de probabilidad. La moda a posteriori de la correlación genética entre sitios fue positiva y alta para las tres características (r=0,7, 0,65 y 0,8, para altura, diámetro y área basal, respectivamente, y de acuerdo al intervalo de credibilidad, esta correlación fue estadísticamente diferente de cero, indicando una interacción no significativa.

  15. Effect of age on calorific value and some mechanical properties of three Eucalyptus species grown in Ethiopia

    Energy Technology Data Exchange (ETDEWEB)

    Mulugeta Lemenih; Tsegaye Bekele [Wondo Genet College of Forestry, Shashemene (Ethiopia)

    2004-09-01

    The effect of age on calorific value, specific gravity, modulus of rupture (MOR) and modulus of elasticity (MOE) of three plantation grown Eucalyptus species in the south-central highlands of Ethiopia was investigated. The species investigated were Eucalyptus globulus Labill., E. saligna Sm. and E grandis W. Hill ex Maid. The investigation was made on sample trees taken from age sequences of stands of the three species. Calorific values were determined on sample discs taken at 4, 8, 12 and 16 m up the stems of the sample trees. Calorific values were also determined for heartwood and sapwood of each disc separately. Specific gravity, MOR and MOE were investigated on a 1 -m long sample log taken from the butt end of each sample tree. The results showed that there was no significant difference in calorific values between the three species when considering a similar age category. Similarly, there was no significant difference in calorific values between disc positions and wood types (heartwood versus sapwood) within species. However, calorific values tend to increase along the tree stem towards the treetop for each of the three species. Correlation between calorific values of each disc position and tree age was generally weak and dominantly negative for E. saligna and E. globulus, but positive for E. grandis. On the other hand, there was a weak but positive correlation between tree age and specific gravity and MOR for the three species. MOE was almost non-correlated with age. Generally, the results showed that waiting for older ages for the three Eucalyptus species may not further increase their calorific values. Hence, stands of the three Eucalyptus species can be harvested and used for fuelwood at an early age as traditionally managed on short-rotation basis. (author)

  16. DENDROMETRY CHARACTERISTICS OF EUCALYPTUS UROPHYLLA IN SILVOPASTORAL SYSTEM UNDER DIFFERENT PLANTING SPACINGS WITH BRACHIARIA DECUMBENS

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    Raphael Pavesi Araujo

    2011-12-01

    Full Text Available The objective of this study was to evaluate the effect of spacing on eucalyptus dendrometric characteristics in silvopastoral system with Brachiaria decumbens. Three eucalyptus spacing were used (3x2, 6x4 and 10x4 m. The randomized block design was used with factorial scheme (3x4 with three densities of planting eucalyptus (3x2, 6x4 and 10x4 m and four times of evaluation (6, 12, 18 and 24 months after planting. The dendrometric characteristics were evaluated: 0,30 m diameter, diameter at breast height - 1.30 m (DBH, cup diameter, height, biomass per tree and plant survival. It was found positive effect of months of evaluation for the diameter to 0.30 m, cup diameter and height. There were no effects of spacing for the variables DBH, survival and biomass per tree when evaluated at 24 months. There was effect for biomass per hectare being 3x2 m the best treatament. It can be concluded that the planting spacing only affected the biomass per hectare, when evaluated up to 24 months of Eucalyptus urophylla grown in silvopastoral system.

  17. Seletivicty of eucalyptus genotypes to herbicides rates / Seletividade de genótipos de eucalipto a doses de herbicidas

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    Siumar Pedro Tironi

    2010-09-01

    Full Text Available The aim of this work was to quantify the herbicide selectivity applied at post-emergence of eucalyptus in different rates. The experiment was conducted in greenhouse conditions in the period from January to March 2007 at the Faculdade de Agronomia Eliseu Maciel in Universidade Federal de Pelotas – Capão do Leão-RS. Experimental design was completely randomized, with four replications. Treatments were in a factorial scheme: factor A - eucalyptus genotypes (E. globulus e E. saligna, factor B - herbicides (isoxaflutole, oxyfluorfen, bispyribac-sodium and sethoxydim+diclosulam, and factor C – rates of the registered average for weed control (0, 50, 100, 150 e 200%. Phytotoxicity, height, stem diameter and dry matter were evaluated. E. globulus genotype was more sensitive to herbicides than E. saligna. The mixture of the herbicides sethoxydim plus diclosulam showed potential to be used in eucalyptus. Bispyribac-sodium herbicide was the most toxic and isoxaflutole was the most selective to the studied genotypes.O objetivo deste trabalho foi quantificar a seletividade de herbicidas aplicados em pós-emergência na cultura do eucalipto em função de diferentes doses. O experimento foi realizado em casa de vegetação, pertencente a Faculdade de Agronomia Eliseu Maciel da Universidade Federal de Pelotas, no Município de Capão do Leão – RS, no período de janeiro a março de 2007. O delineamento experimental utilizado foi completamente casualizado, com quatro repetições. Os tratamentos foram arranjados em esquema fatorial, onde o fator A testou genótipos de eucalipto (E. globulus e E. saligna, o fator B comparou quatro herbicidas (isoxaflutole, oxyfluorfen, bispyribac-sodium e sethoxydim+diclosulam e o fator C doses (0, 50, 100, 150 e 200% da dose média registrada para controle de plantas daninhas. As variáveis avaliadas foram fitotoxicidade, estatura, diâmetro do caule e matéria seca da parte aérea das plantas de eucalipto. O gen

  18. Impacts of population structure and analytical models in genome-wide association studies of complex traits in forest trees: a case study in Eucalyptus globulus.

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    Eduardo P Cappa

    Full Text Available The promise of association genetics to identify genes or genomic regions controlling complex traits has generated a flurry of interest. Such phenotype-genotype associations could be useful to accelerate tree breeding cycles, increase precision and selection intensity for late expressing, low heritability traits. However, the prospects of association genetics in highly heterozygous undomesticated forest trees can be severely impacted by the presence of cryptic population and pedigree structure. To investigate how to better account for this, we compared the GLM and five combinations of the Unified Mixed Model ( UMM on data of a low-density genome-wide association study for growth and wood property traits carried out in a Eucalyptus globulus population (n = 303 with 7,680 Diversity Array Technology (DArT markers. Model comparisons were based on the degree of deviation from the uniform distribution and estimates of the mean square differences between the observed and expected p-values of all significant marker-trait associations detected. Our analysis revealed the presence of population and family structure. There was not a single best model for all traits. Striking differences in detection power and accuracy were observed among the different models especially when population structure was not accounted for. The UMM method was the best and produced superior results when compared to GLM for all traits. Following stringent correction for false discoveries, 18 marker-trait associations were detected, 16 for tree diameter growth and two for lignin monomer composition (S:G ratio, a key wood property trait. The two DArT markers associated with S:G ratio on chromosome 10, physically map within 1 Mbp of the ferulate 5-hydroxylase (F5H gene, providing a putative independent validation of this marker-trait association. This study details the merit of collectively integrate population structure and relatedness in association analyses in undomesticated, highly

  19. Impacts of Population Structure and Analytical Models in Genome-Wide Association Studies of Complex Traits in Forest Trees: A Case Study in Eucalyptus globulus

    Science.gov (United States)

    Garcia, Martín N.; Acuña, Cintia; Borralho, Nuno M. G.; Grattapaglia, Dario; Marcucci Poltri, Susana N.

    2013-01-01

    The promise of association genetics to identify genes or genomic regions controlling complex traits has generated a flurry of interest. Such phenotype-genotype associations could be useful to accelerate tree breeding cycles, increase precision and selection intensity for late expressing, low heritability traits. However, the prospects of association genetics in highly heterozygous undomesticated forest trees can be severely impacted by the presence of cryptic population and pedigree structure. To investigate how to better account for this, we compared the GLM and five combinations of the Unified Mixed Model (UMM) on data of a low-density genome-wide association study for growth and wood property traits carried out in a Eucalyptus globulus population (n = 303) with 7,680 Diversity Array Technology (DArT) markers. Model comparisons were based on the degree of deviation from the uniform distribution and estimates of the mean square differences between the observed and expected p-values of all significant marker-trait associations detected. Our analysis revealed the presence of population and family structure. There was not a single best model for all traits. Striking differences in detection power and accuracy were observed among the different models especially when population structure was not accounted for. The UMM method was the best and produced superior results when compared to GLM for all traits. Following stringent correction for false discoveries, 18 marker-trait associations were detected, 16 for tree diameter growth and two for lignin monomer composition (S∶G ratio), a key wood property trait. The two DArT markers associated with S∶G ratio on chromosome 10, physically map within 1 Mbp of the ferulate 5-hydroxylase (F5H) gene, providing a putative independent validation of this marker-trait association. This study details the merit of collectively integrate population structure and relatedness in association analyses in undomesticated, highly

  20. Plants of an Eucalyptus clone damaged by Scolytidae and Platypodidae (Coleoptera Plantas de Eucalyptus atacadas por Scolytidae e Platypodidae (Coleoptera

    Directory of Open Access Journals (Sweden)

    José Cola Zanuncio

    2005-05-01

    Full Text Available Species of Scolytidae and Platypodidae were evaluated in a plantation using a clone of Eucalyptus grandis x Eucalyptus urophylla in the State of Minas Gerais, Brazil. Wood disks with galleries were taken from 15 eucalyptus trees between 0.5 and 1.5 m height. After collection, all trees attacked were burned. Woodborers obtained from these disks were identified as Premnobius cavipennis, Premnobius ambitiosus, Dryocoetoides cristatus (Coleoptera: Scolytidae and Euplatypus parallelus (Coleoptera: Platypodidae. This is the first report of D. cristatus, a Brazilian native species, damaging eucalyptus, which shows its adaptation to this plant.O objetivo deste trabalho foi avaliar o ataque de Scolytidae e Platypodidae em talhão de clone Eucalyptus grandis x Eucalyptus urophylla na região de Montes Claros, Estado de Minas Gerais, Brasil. Discos de madeira com galerias desses insetos foram retirados de 15 árvores de eucalipto entre 0,5 e 1,5 m de altura. Após a coleta deste material, as árvores atacadas foram queimadas. Os indivíduos da ordem Coleoptera obtidos desses discos foram identificados como Premnobius cavipennis, Premnobius ambitiosus e Dryocoetoides cristatus (Coleoptera: Scolytidae e Euplatypus parallelus (Coleoptera: Platypodidae. O ataque de D. cristatus, espécie nativa do Brasil, ao eucalipto ainda não havia sido relatado, o que mostra sua adaptação a essa planta.

  1. Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species

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    Cintia V. Acuña

    2014-12-01

    Full Text Available Aim of study:  To analyze the feasibility of extrapolating conclusions on wood quality genetic control between different Eucalyptus species, particularly from species with better genomic information, to those less characterized. For this purpose, the first step is to analyze the conservation and cross-transferability of microsatellites markers (SSRs located in candidate genes.Area of study: Eucalyptus species implanted in Argentina coming from different Australian origins.Materials and methods: Twelve validated and polymorphic SSRs in candidate genes (SSR-CGs for wood quality in E. globulus were selected for cross species amplification in six species: E. grandis, E. saligna, E. dunnii, E. viminalis, E. camaldulensis and E. tereticornis.Main results: High cross-species transferability (92% to 100% was found for the 12 polymorphic SSRs detected in E. globulus. These markers revealed allelic diversity in nine important candidate genes: cinnamoyl CoA reductase (CCR, cellulose synthase 3 (CesA3, the transcription factor LIM1, homocysteine S-methyltransferase (HMT, shikimate kinase (SK, xyloglucan endotransglycosylase 2 (XTH2, glutathione S-transferase (GST, glutamate decarboxylase (GAD and peroxidase (PER.Research highlights: The markers described are potentially suitable for comparative QTL mapping, molecular marker assisted breeding (MAB and for population genetic studies across different species within the subgenus Symphyomyrtus.Keywords: validation; cross-transferability; SSR; functional markers; eucalypts; Symphyomyrtus.

  2. VARIABILIDAD Y RELACION ENTRE LAS PROPIEDADES ANATOMICAS, QUIMICAS Y LA APTITUD PULPABLE, EN CLONES DE EUCALYPTUS GLOBULUS.

    OpenAIRE

    RAMIREZ VIDAL, MAURICIO HERNAN; RAMIREZ VIDAL, MAURICIO HERNAN

    2008-01-01

    Se estudió la variación de la anatomía de la madera, variables biornétricas, aptitud pulpable y propiedades de la pulpa, en 14 clones de E. globulus de 7 años de edad, creciendo en un ensayo clonal en la zona de Arauco, región del Bio-Bio, Chile. Las Cara 61p.

  3. Insecticidal Activity of Some Traditionally Used Ethiopian Medicinal Plants against Sheep Ked Melophagus ovinus

    OpenAIRE

    Gemeda, Negero; Mokonnen, Walelegn; Lemma, Hirut; Tadele, Ashenif; Urga, Kelbessa; Addis, Getachew; Debella, Asfaw; Getachew, Mesaye; Teka, Frehiwot; Yirsaw, Kidist; Mudie, Kissi; Gebre, Solomon

    2014-01-01

    Twelve medicinal plants and a commercially used drug Ivermectin were examined for insecticidal activity against Melophagus ovinus sheep ked at different time intervals using in vitro adult immersion test. The findings show that at 3.13 µL/mL, 6.25 µL/mL and 12.5 µL/mL concentration of Cymbopogon citratus, Foeniculum vulgare and Eucalyptus globulus essential oils respectively, recorded 100% mortalities against M. ovinus within 3 hour of exposure. Significantly higher insecticidal activity of e...

  4. Dry mass allocation, water use efficiency and delta C-13 in clones of Eucalyptus grandis, E-grandis x camaldulensis and E-grandis x nitens grown under two irrigation regimes

    CSIR Research Space (South Africa)

    Le Roux, D

    1996-05-01

    Full Text Available - cial clones of Eucalyptus grandis W. Hill ex Maiden. implying that less water-use-efficient trees were more productive (Bond and Stock 1990). Similarly, growing season WUE and delta13C were positively correlated in western larch and Eucalyptus globulus... regimes DEBBIE LE ROUX,1,2 WILLIAM D. STOCK,3 WILLIAM J. BOND3 and DAVID MAPHANGA4 1 Division of Forest Science and Technology, CSIR, Pretoria, South Africa 2 Present address: Department of Botany, University of Kansas, Lawrence, KS 66045, USA 3 Department...

  5. Soil microbial community structure and function responses to successive planting of Eucalyptus.

    Science.gov (United States)

    Chen, Falin; Zheng, Hua; Zhang, Kai; Ouyang, Zhiyun; Li, Huailin; Wu, Bing; Shi, Qian

    2013-10-01

    Many studies have shown soil degradation after the conversion of native forests to exotic Eucalyptus plantations. However, few studies have investigated the long-term impacts of short-rotation forestry practices on soil microorganisms. The impacts of Eucalyptus successive rotations on soil microbial communities were evaluated by comparing phospholipid fatty acid (PLFA) abundances, compositions, and enzyme activities of native Pinus massoniana plantations and adjacent 1st, 2nd, 3rd, 4th generation Eucalyptus plantations. The conversion from P. massoniana to Eucalyptus plantations significantly decreased soil microbial community size and enzyme activities, and increased microbial physiological stress. However, the PLFA abundances formed "u" shaped quadratic functions with Eucalyptus plantation age. Alternatively, physiological stress biomarkers, the ratios of monounsaturated to saturated fatty acid and Gram+ to Gram- bacteria, formed "n"' shaped quadratic functions, and the ratio of cy17:0 to 16:1omega7c decreased with plantation age. The activities of phenol oxidase, peroxidase, and acid phosphatase increased with Eucalyptus plantation age, while the cellobiohydrolase activity formed "u" shaped quadratic functions. Soil N:P, alkaline hydrolytic nitrogen, soil organic carbon, and understory cover largely explained the variation in PLFA profiles while soil N:P, alkaline hydrolytic nitrogen, and understory cover explained most of the variability in enzyme activity. In conclusion, soil microbial structure and function under Eucalyptus plantations were strongly impacted by plantation age. Most of the changes could be explained by altered soil resource availability and understory cover associated with successive planting of Eucalyptus. Our results highlight the importance of plantation age for assessing the impacts of plantation conversion as well as the importance of reducing disturbance for plantation management.

  6. Ensaios clínicos toxicológicos, fase I, de um fitoterápico composto (Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour e Eucalyptus globulus Labill

    Directory of Open Access Journals (Sweden)

    Patrícia Trindade C. Paulo

    Full Text Available Neste estudo, foram realizados ensaios clínicos toxicológicos, fase I, do produto fitoterápico composto pelas plantas medicinais Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour e Eucalyptus globulus Labill. O estudo foi realizado no Hospital Universitário Lauro Wanderley/UFPB/PB e, para isto, foram selecionados 28 voluntários sadios, sendo 14 homens e 14 mulheres que ingeriram por via oral, ininterruptamente durante 8 semanas, 15 mL do produto, três vezes ao dia; e no 3º e 7º dia, 3ª e 6ª semanas e 24 h após a 8ª semana, foram feitas avaliações clínicas e laboratoriais para análise da toxicidade aguda e crônica. Os resultados obtidos demonstraram que os pacientes não apresentaram alterações clínicas, laboratoriais e reações adversas significantes, apenas pequenas alterações foram detectadas no sangue através da aspartato transaminase (AST e fosfatase alcalina no grupo feminino para um p < 0,05; no entanto, estes valores determinados permaneceram dentro dos valores de normalidade para indivíduos adultos. Conclui-se que estes dados, em complementação àqueles obtidos com os estudos pré-clínicos, confirmam a baixa toxicidade do produto fitoterápico.

  7. Genome-wide variation in recombination rate in Eucalyptus.

    Science.gov (United States)

    Gion, Jean-Marc; Hudson, Corey J; Lesur, Isabelle; Vaillancourt, René E; Potts, Brad M; Freeman, Jules S

    2016-08-09

    Meiotic recombination is a fundamental evolutionary process. It not only generates diversity, but influences the efficacy of natural selection and genome evolution. There can be significant heterogeneity in recombination rates within and between species, however this variation is not well understood outside of a few model taxa, particularly in forest trees. Eucalypts are forest trees of global economic importance, and dominate many Australian ecosystems. We studied recombination rate in Eucalyptus globulus using genetic linkage maps constructed in 10 unrelated individuals, and markers anchored to the Eucalyptus reference genome. This experimental design provided the replication to study whether recombination rate varied between individuals and chromosomes, and allowed us to study the genomic attributes and population genetic parameters correlated with this variation. Recombination rate varied significantly between individuals (range = 2.71 to 3.51 centimorgans/megabase [cM/Mb]), but was not significantly influenced by sex or cross type (F1 vs. F2). Significant differences in recombination rate between chromosomes were also evident (range = 1.98 to 3.81 cM/Mb), beyond those which were due to variation in chromosome size. Variation in chromosomal recombination rate was significantly correlated with gene density (r = 0.94), GC content (r = 0.90), and the number of tandem duplicated genes (r = -0.72) per chromosome. Notably, chromosome level recombination rate was also negatively correlated with the average genetic diversity across six species from an independent set of samples (r = -0.75). The correlations with genomic attributes are consistent with findings in other taxa, however, the direction of the correlation between diversity and recombination rate is opposite to that commonly observed. We argue this is likely to reflect the interaction of selection and specific genome architecture of Eucalyptus. Interestingly, the differences amongst

  8. Wood density and anatomy of three Eucalyptus species: implications for hydraulic conductivity

    International Nuclear Information System (INIS)

    Barotto, A.J.; Monteoliva, S.; Gyenge, J.; Martínez-Meier, A.; Moreno, K.; Tesón, N.; Fernández, M.E.

    2017-01-01

    Aim of the study: To characterize wood anatomical traits of three Eucalyptus species that differ in wood density and ecological requirements, and to examine the relationships between some anatomical features, wood density, and theoretical xylem hydraulic conductivity (Ks). Area of study: We analyzed 86 trees from three sites of Argentina (Entre Ríos and Buenos Aires Provinces). Methods: The sampled trees were Eucalyptus globulus, E. grandis and E. viminalis ranging from 11 to 15 years old. One stem disc was cut from each tree to determine wood density and identify quantitative anatomical features of vessels and fibers. Vessel composition (S, size - to-number ratio, a measure of vessel size distribution) and lumen fraction (F, the total sapwood area available for water transport) were estimated. Results: E. grandis, the species with the highest growth rates, presented the highest theoretical Ks. This was associated with anatomical features such as a high density of wide vessels resulting in high F. On the other hand, E. viminalis, the species with the lowest growth rates and highest resistance to environmental stress, showed lower Ks as a result of a low density of wide vessels. These two species differed not only greatly in wood density but also in fiber characteristics. In the case of E. globulus, vessels were relatively narrow, which resulted in the lowest theoretical Ks, fibers were small, and wood density intermediate. Research highlights: F had greater influence on Ks than S. The anatomical characteristics and wood density could only partly explain the differential growth or resistance to stress of the studied species.

  9. Wood density and anatomy of three Eucalyptus species: implications for hydraulic conductivity

    Directory of Open Access Journals (Sweden)

    Antonio J. Barotto

    2017-05-01

    Full Text Available Aim of the study: To characterize wood anatomical traits of three Eucalyptus species that differ in wood density and ecological requirements, and to examine the relationships between some anatomical features, wood density, and theoretical xylem hydraulic conductivity (Ks. Area of study: We analyzed 86 trees from three sites of Argentina (Entre Ríos and Buenos Aires Provinces. Methods: The sampled trees were Eucalyptus globulus, E. grandis and E. viminalis ranging from 11 to 15 years old. One stem disc was cut from each tree to determine wood density and identify quantitative anatomical features of vessels and fibers. Vessel composition (S, size - to-number ratio, a measure of vessel size distribution and lumen fraction (F, the total sapwood area available for water transport were estimated. Results: E. grandis, the species with the highest growth rates, presented the highest theoretical Ks. This was associated with anatomical features such as a high density of wide vessels resulting in high F. On the other hand, E. viminalis, the species with the lowest growth rates and highest resistance to environmental stress, showed lower Ks as a result of a low density of wide vessels. These two species differed not only greatly in wood density but also in fiber characteristics. In the case of E. globulus, vessels were relatively narrow, which resulted in the lowest theoretical Ks, fibers were small, and wood density intermediate. Research highlights: F had greater influence on Ks than S. The anatomical characteristics and wood density could only partly explain the differential growth or resistance to stress of the studied species.

  10. Wood density and anatomy of three Eucalyptus species: implications for hydraulic conductivity

    Energy Technology Data Exchange (ETDEWEB)

    Barotto, A.J.; Monteoliva, S.; Gyenge, J.; Martínez-Meier, A.; Moreno, K.; Tesón, N.; Fernández, M.E.

    2017-11-01

    Aim of the study: To characterize wood anatomical traits of three Eucalyptus species that differ in wood density and ecological requirements, and to examine the relationships between some anatomical features, wood density, and theoretical xylem hydraulic conductivity (Ks). Area of study: We analyzed 86 trees from three sites of Argentina (Entre Ríos and Buenos Aires Provinces). Methods: The sampled trees were Eucalyptus globulus, E. grandis and E. viminalis ranging from 11 to 15 years old. One stem disc was cut from each tree to determine wood density and identify quantitative anatomical features of vessels and fibers. Vessel composition (S, size - to-number ratio, a measure of vessel size distribution) and lumen fraction (F, the total sapwood area available for water transport) were estimated. Results: E. grandis, the species with the highest growth rates, presented the highest theoretical Ks. This was associated with anatomical features such as a high density of wide vessels resulting in high F. On the other hand, E. viminalis, the species with the lowest growth rates and highest resistance to environmental stress, showed lower Ks as a result of a low density of wide vessels. These two species differed not only greatly in wood density but also in fiber characteristics. In the case of E. globulus, vessels were relatively narrow, which resulted in the lowest theoretical Ks, fibers were small, and wood density intermediate. Research highlights: F had greater influence on Ks than S. The anatomical characteristics and wood density could only partly explain the differential growth or resistance to stress of the studied species.

  11. Isolation and Physicochemical Characterization of Lignin from ...

    African Journals Online (AJOL)

    Muzakir

    and properties, lignin offers a perspective for higher value-added ... priority for the development and implementation of the lignocellulosic ... of about 2.5 m and adaptable to most soils. It had ..... in young versus adult Eucalyptus globulus plants.

  12. Bioactivity of essential oils from medicinal plants of Cameroon and ...

    African Journals Online (AJOL)

    The EOs of Eucalyptus globulus, Cymbopogon citratus, Xylopia aethiopica, Thymus vulgaris, Ocimum canum, Cananga odorata, Citrus medica, Citrus paradisi and Citrus reticulata were screened against the most incriminated bacterial species using the spot-on-agar test and microdilution methods. Some of the EOs with ...

  13. [Growth effect of eucalyptus-acacia mixed plantation in South China].

    Science.gov (United States)

    Yang, Zeng-Jiang; Xu, Da-Ping; Chen, Wen-Ping; Huang, Lie-Jian; Li, Shang-Jun; Chen, Yuan

    2009-10-01

    Eucalyptus U6 and Acacia crassicarpa were mixed planted with different ratios and modes to investigate the growth parameters of the two tree species. In the 2-3 years old mixed plantation, the wind-throw of A. crassicarpa decreased markedly with increasing ratio of Eucalyptus U6, the decrement being 26.14% when the Eucalyptus U6/A. crassicarpa ratio was 3 : 1, but the survival rates of Eucalyptus U6 and A. crassicarpa had no significant difference under different planting modes. Mixed planting retarded the A. crassicarpa growth to some extent, with the DBH being 90% of that in pure A. crassicarpa stand. The mixed planting had little effects on the height growth of Eucalyptus U6, but promoted its DBH growth markedly, and the beneficial effect increased with increasing ratio of A. crassicarpa. In the 6 years old 1 : 1 Eucalyptus U6/A. crassicarpa plantation, the Eucalyptus U6 individuals with DBH > 15 cm occupied 32.1%; while in pure Eucalyptus U6 stand, they only accounted for 5.83%. Mixed planting with 2 : 1 Eucalyptus U6/A. crassicarpa could obtain a maximum total biomass of 198.8 m3 x hm(-2), which was 118.8% of the total biomass in pure Eucalyptus U6 stand, or 169.9% of that in pure A. crassicarpa stand. Mixture of Eucalyptus with Acacia would be a good choice to produce Eucalyptus trees with larger DBH.

  14. In vitro antifugal activity of medicinal plant extract against Fusarium oxysporum f. sp. lycopersici race 3 the causal agent of tomato wilt.

    Science.gov (United States)

    Isaac, G S; Abu-Tahon, M A

    2014-03-01

    Medicinal plant extracts of five plants; Adhatoda vasica, Eucalyptus globulus, Lantana camara, Nerium oleander and Ocimum basilicum collected from Cairo, Egypt were evaluated against Fusarium oxysporum f. sp. lycopersici race 3 in vitro conditions using water and certain organic solvents. The results revealed that cold distilled water extracts of O. basilicum and E. globulus were the most effective ones for inhibiting the growth of F. oxysporum f. sp. lycopersici. Butanolic and ethanolic extracts of the tested plants inhibited the pathogen growth to a higher extent than water extracts. Butanolic extract of O. basilicum completely inhibited the growth of F. oxysporum f. sp. lycopersici at concentrations 1.5 and 2.0% (v/v). Butanolic extracts (2.0%) of tested plants had a strong inhibitory effect on hydrolytic enzymes; β-glucosidase, pectin lyase and protease of F. oxysporum f. sp. lycopersici. This study has confirmed that the application of plant extracts, especially from O. basilicum for controlling F. oxysporum f. sp. lycopersici is environmentally safe, cost effective and does not disturb ecological balance. Investigations are in progress to test the efficacy of O. basilicum extract under in vivo conditions.

  15. Characterization and Identification of the Basidiomycetous Fungus Associated with 'hoya de malvón' Grapevine Disease in Argentina

    Directory of Open Access Journals (Sweden)

    S. Lupo

    2006-04-01

    Full Text Available Inocutis jamaicensis (Murrill Gottlieb, J.E. Wright & Moncalvo was identified as the basidiomycetous species associated with ‘hoja de malvón’ grapevine disease in Argentina. Macro and micro-morphological characteristics of fruit bodies corresponded to those described for the white-rotting fungus associated with native plant species and Eucalyptus globulus Labill. planted in Uruguay. Monokariotic isolates were obtained from basidiospores produced by fruit bodies of I. jamaicensis collected from Vitis vinifera L. and E. globulus. Dikaryons and fruit bodies produced by pairing monokaryotic mycelium suggest that all these isolates belong to the same species. The analysis of RFLP of the dikaryon produced by pairing monokaryons derived from V. vinifera and E. globulus revealed fragments that corresponded to each monokaryon, confirming that isolates from Vitis mated with those from Eucalyptus. In order to compare grapevine and Uruguayan isolates, RFLPs from ITS region generated by restriction digestion with Alu I, Hae III, Hha I, Msp I and Taq I were performed. Differences found in some restriction pattern could reflect a certain degree of variability between dikariotic isolates, probably related with a particular lifestyle, host specificity or geographic origin.

  16. MODELING THE GROWTH OF EUCALYPTUS PLANTS BASED ON THE THERMAL SUM

    Directory of Open Access Journals (Sweden)

    Aline Santana de Oliveira

    Full Text Available ABSTRACT Among the environmental variables that affect the growth and development of plants, the air temperature is of great importance. In this context, the objectives of this work were to model the growth of eucalyptus seedlings in terms of accumulated degree-days during the production process and model validation. The study was conducted in the forest research nursery of the Department of Forestry, located in Viçosa (MG, during the periods of 08/02/2011 to 28/04/2011 and 03/08/2012 to 01/11/2012, making it possible to contemplate seasonal variations in the production cycle. The monitored variables were shoot height, stem diameter, leaf area, root length and fresh and dry biomass. Results showed that it took 1065 degree-days for the production of seedlings and sigmoidal models obtained showed high correlation and Willmott coefficients, indicating good performance for estimating the growth and development of eucalyptus seedlings. This tool has great potential for planning and monitoring the production of eucalyptus seedlings in nurseries.

  17. Responses to mild water deficit and rewatering differ among secondary metabolites but are similar among provenances within Eucalyptus species.

    Science.gov (United States)

    McKiernan, Adam B; Potts, Brad M; Brodribb, Timothy J; Hovenden, Mark J; Davies, Noel W; McAdam, Scott A M; Ross, John J; Rodemann, Thomas; O'Reilly-Wapstra, Julianne M

    2016-02-01

    Water deficit associated with drought can severely affect plants and influence ecological interactions involving plant secondary metabolites. We tested the effect of mild water deficit and rewatering on physiological, morphological and chemical traits of juvenile Eucalyptus globulus Labill. and Eucalyptus viminalis Labill. We also tested if responses of juvenile eucalypts to water deficit and rewatering varied within species using provenances across a rainfall gradient. Both species and all provenances were similarly affected by mild water deficit and rewatering, as only foliar abscisic acid levels differed among provenances during water deficit. Across species and provenances, water deficit decreased leaf water potential, above-ground biomass and formylated phloroglucinol compound concentrations, and increased condensed tannin concentrations. Rewatering reduced leaf carbon : nitrogen, and total phenolic and chlorogenic acid concentrations. Water deficit and rewatering had no effect on total oil or individual terpene concentrations. Levels of trait plasticity due to water deficit and rewatering were less than levels of constitutive trait variation among provenances. The overall uniformity of responses to the treatments regardless of native provenance indicates limited diversification of plastic responses when compared with the larger quantitative variation of constitutive traits within these species. These responses to mild water deficit may differ from responses to more extreme water deficit or to responses of juvenile/mature eucalypts growing at each locality. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  18. Effect of Echinophora platyloba , Stachys lavandulifolia, and Eucalyptus camaldulensis plants on Trichomonas vaginalis growth in vitro

    Directory of Open Access Journals (Sweden)

    Hossein Ali Youse

    2012-01-01

    Full Text Available Background: Trichomonas vaginalis is a protozoan parasite which causes vaginitis in women worldwide. Metronidazole with vast side effects is drug of choice for this infection. In search for an alternative drug, in this study the effect of three plants on Trichomonas vaginalis has been investigated in vitro. Materials and Methods: Alcoholic and watery extracts of Echinophora platyloba, Stachys lavandulifolia, and Eucalyptus camaldulensis were prepared. In TYIS culture medium containing alive Trichomonas vaginalis different concentrations of extracts of three plants were added. Following, 24, 48, and 72 h incubation the number of parasite in each test tube was counted. Results: Eucalyptus camaldulensis showed a strong effect on Trichomonas vaginalis growth. However, no significant effect was observed with Echinophora platyloba or Stachys lavandulifolia extracts. Conclusion: Eucalyptus camaldulensis can be considered as an alternative drug for treatment of infective vaginitis which is caused by bacteria, fungi and parasites.

  19. Effects of some anti-diabetic plants on the hepatic marker enzymes ...

    African Journals Online (AJOL)

    This study was embarked upon in order to evaluate the effects of the chloroform extracts of the leaves of Psidium guajava, Anacardium occidentale and Eucalyptus globulus and fruits of Xylopia aethiopica on hepatic marker enzymes of diabetic rats. The degree of hepatic damage caused by diabetes mellitus and the effects ...

  20. Eucalyptus oil nanoemulsion-impregnated chitosan film: antibacterial effects against a clinical pathogen, Staphylococcus aureus, in vitro

    Directory of Open Access Journals (Sweden)

    Sugumar S

    2015-10-01

    Full Text Available Saranya Sugumar, Amitava Mukherjee, Natarajan Chandrasekaran Centre for Nanobiotechnology, VIT University, Vellore, India Abstract: Eucalyptus oil (Eucalyptus globulus nanoemulsion was formulated using low- and high-energy emulsification methods. Development of nanoemulsion was optimized for system parameters such as emulsifier type, emulsifier concentration, and emulsification methods to obtain a lower droplet size with greater stability. The minimized droplet diameter was achieved using the high-energy method of ultrasonication. Tween 80 was more effective in reducing droplet size and emulsion appearance when compared to Tween 20. Stable nanoemulsion was formulated with Tween 80 as a surfactant, and the particle size was found to be 9.4 nm (1:2 v/v. The eucalyptus oil nanoemulsion was impregnated into chitosan (1% as a biopolymer in varying concentrations. Further, the film was characterized by moisture content, microscopic study, X-ray diffraction, and Fourier transform infrared spectroscopy. Also, the film with and without nanoemulsion was evaluated against Staphylococcus aureus. The nanoemulsion-impregnated chitosan film showed higher antibacterial activity than chitosan film. These results support the inclusion of nanoemulsion-impregnated chitosan film in wound management studies. Keywords: essential oil, emulsion, biopolymer, impregnation, thin film, wound isolate

  1. Amenability of Acacia and Eucalyptus Hardwood Pulps to Elemental Chlorine-Free Bleaching: Application and Efficacy of Microbial Xylanase

    Directory of Open Access Journals (Sweden)

    Avdhesh Kumar Gangwar

    2015-10-01

    Full Text Available This study outlines the results of a biobleaching study of acacia (A. mangium and eucalyptus (E. globulus hardwood kraft pulps with commercial xylanase (Optimase CX 72 L. The comparative study was carried out using an elemental chlorine-free (ECF bleaching sequence (D0EPD1D2 after the enzyme (X stage. The enzyme treatment resulted in improved optical properties with a reduction in bleach chemical consumption. At an equivalent bleach chemical consumption, a brightness gain of 2.1 and 1.7 units and a whiteness gain of 2.7 and 2.3 units were observed with xylanase treatment in acacia and eucalyptus pulps, respectively. In ECF bleaching using the D0EPD1D2 sequence, a final brightness was achieved to the extent of 90% ISO and 89% ISO for acacia and eucalyptus, respectively, at an equivalent charge of bleach chemicals. The post-color (PC number was also reduced by up to 45% for both hardwood pulps compared with the control. The bleachability of acacia was observed to be significantly higher than that of eucalyptus. In addition, a 17.0% and 23.0% reduction in chlorine dioxide and sodium hydroxide, respectively, were obtained for both hardwood pulps after xylanase pre-bleaching, thus indicating an environmentally friendly approach to the process.

  2. Marker-assisted selection in Eucalyptus

    International Nuclear Information System (INIS)

    Grattapaglia, D.

    2007-01-01

    Planted Eucalyptus occupies globally more than 18 million hectares and has become the most widely planted hardwood tree in the world, supplying high-quality woody biomass for several industrial applications. In this chapter an overview is presented on the status and perspectives of marker-assisted selection (MAS) in species of Eucalyptus. After an introduction to the main features of modern eucalypt breeding and clonal forestry, some applications of molecular markers in support to operational breeding are presented. By reviewing the status of quantitative trait locus (QTL) mapping in Eucalyptus, the challenges and some realistic prospects for the application of MAS to improve relevant traits are outlined. With the expected availability of more powerful genomic tools, including a draft of the Eucalyptus genome, the main challenges in implementing MAS will be in phenotyping trees accurately, analysing the overwhelming amount of genomic data available and translating this into truly useful molecular tools for breeding. (author)

  3. Actinomycetes from Eucalyptus and their biological activities for controlling Eucalyptus leaf and shoot blight.

    Science.gov (United States)

    Himaman, Winanda; Thamchaipenet, Arinthip; Pathom-Aree, Wasu; Duangmal, Kannika

    2016-01-01

    In Thailand, Eucalyptus plantations rapidly expand across the country. Leaf and shoot blight caused by Cryptosporiopsis eucalypti, Cylindrocladium sp. and Teratosphaeria destructans is a serious disease in Eucalyptus plantations. In this study, a total of 477 actinomycete strains were successfully isolated from roots and rhizosphere soil of Eucalyptus. Four hundred and thirty nine isolates were classified as streptomycetes and 38 isolates were non-streptomycetes. Among these isolates, 272 (57.0%), 118 (24.7%) and 241 (50.5%) isolates were antagonistic to Cryptosporiopsis eucalypti, Cylindrocladium sp. and Teratosphaeria destructans, respectively. All isolates were tested for their abilities to produce siderophores, indole acetic acid (IAA) and solubilise phosphate. Most isolates (464, 97.3%) produced siderophores. The majority of isolates (345, 72.3%) solubilised phosphate. In addition, almost half of these isolates (237, 49.7%) produced indole acetic acid. Strain EUSKR2S82 which showed the strongest inhibitory effect against all tested fungi with plant growth promoting ability was selected to test with Eucalyptus. This strain could colonize plant roots and increase Eucalyptus roots length. In a detached leaves bioassay, the disease severity of EUSKR2S82-inoculated Eucalyptus leaves was only 30% compared to 95% in the control treatment. The 16S rRNA gene sequence analysis revealed that the strain EUSKR2S82 was related to Streptomyces ramulosus NRRL-B 2714(T) (99.44% similarity). Identification of non-streptomycete isolates using 16S rRNA gene sequences classified them into 9 genera: Actinoallomurus, Actinomadura, Amycolatopsis, Cryptosporangium, Microbispora, Micromonospora, Nocardia, Nonomuraea and Pseudonocardia. It is evident that Eucalyptus tree harbored several genera of actinomycetes. The selected isolate, EUSKR2S82 showed potential as a candidate for biocontrol agent of leaf and shoot blight of Eucalyptus and to promote growth. Copyright © 2016 Elsevier Gmb

  4. Natural Plant Essential Oils for Controlling the Grasshopper (Heteracris littoralis and their Pathological Effects on the Alimentary Canal

    Directory of Open Access Journals (Sweden)

    Aziza Sharaby

    2012-07-01

    Full Text Available In the present study, the toxic effect of three different natural essential oils of medicinal plants, namely Garlic (Allium sativum, Mint (Mintha pipereta and Eucalyptus (Eucalyptus globulus were tested on 1st nymphal instar of the grasshopper (Heteracris littoralis. The LC50 values of the tested oils were estimated after 14 days from feeding on treated diet mixed with different concentrations of the oil. The LC50 of the tested oils were arranged as follows: 0.067, 0.075 and 0.084ml. /100ml. diet for Garlic, Eucalyptus and Mint respectively. The effect of LC50 concentration of the oils on the biological aspects and histological changes that observed on the alimentary canal and fat bodies were recorded. The normal development of the grasshopper was exhibited. Results cleared that there was statistical variable numbers of increased the nymphal periods, life cycle, adults longevity and life span comparing with the control test. Garlic oil inhibited egg lying by the resulting females offspring of the treated1st instar nymphs. High reduction in the deposited eggs and egg fertility caused by Eucalyptus or Mint oil and marked malformation were observed. Histological changes on the alimentary canal and fat bodies of the remaining nymphs after treatment with Garlic oil (the most effective oil were detected by the light microscope have been recorded. The results suggest that the natural plant essential oils of Garlic, Eucalyptus and Mint may be used in IPM control program against H. littoralis grasshopper.

  5. Furfural production from Eucalyptus wood using an Acidic Ionic Liquid.

    Science.gov (United States)

    Peleteiro, Susana; Santos, Valentín; Garrote, Gil; Parajó, Juan Carlos

    2016-08-01

    Eucalyptus globulus wood samples were treated with hot, compressed water to separate hemicelluloses (as soluble saccharides) from a solid phase mainly made up of cellulose and lignin. The liquid phase was dehydrated, and the resulting solids (containing pentoses as well as poly- and oligo- saccharides made up of pentoses) were dissolved and reacted in media containing an Acidic Ionic Liquid (1-butyl-3-methylimidazolium hydrogen sulfate) and a co-solvent (dioxane). The effects of the reaction time on the product distribution were studied at temperatures in the range 120-170°C for reaction times up to 8h, and operational conditions leading to 59.1% conversion of the potential substrates (including pentoses and pentose structural units in oligo- and poly- saccharides) into furfural were identified. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Development of a Biochar-Plant-Extract-Based Nitrification Inhibitor and Its Application in Field Conditions

    Directory of Open Access Journals (Sweden)

    Jhónatan Reyes-Escobar

    2015-10-01

    Full Text Available The global use of nitrogen (N fertilizer has increased 10-fold in the last fifty years, resulting in increased N losses via nitrate leaching to groundwater bodies or from gaseous emissions to the atmosphere. One of the biggest problems farmers face in agricultural production systems is the loss of N. In this context, novel biological nitrification inhibitors (BNI using biochar (BC as a renewable matrix to increase N use efficiency, by reducing nitrification rates, have been evaluated. The chemical and morphological characteristics of BC were analyzed and BC-BNI complexes were formulated using plant extracts from pine (Pinus radiata, eucalyptus (Eucalyptus globulus and peumo (Cryptocarya alba. In field experiments, fertilizer and treatments, based on crude plant extracts and BC-BNI complexes, were applied and the effect on nitrification was periodically monitored, and at the laboratory level, a phytotoxicity assay was performed. The biochar-peumo (BCPe complex showed the highest nitrification inhibition (66% on day 60 after application compared with the crude plant extract, suggesting that BCPe complex protects the BNI against biotic or abiotic factors, and therefore BC-BNI complexes could increase the persistence of biological nitrification inhibitors. None of the biochar complexes had toxic effect on radish plants.

  7. Eucalyptus microsatellites mined in silico

    Indian Academy of Sciences (India)

    https://www.ias.ac.in/article/fulltext/jgen/087/01/0021-0025 ... Eucalyptus is an important short rotation pulpy woody plant, grown widely in the tropics. ... In this study, in silico analysis of 15,285 sequences representing partial and full-length mRNA from Eucalyptus species for their use in developing SSRs or microsatellites ...

  8. Forest nursery management in Chile

    Science.gov (United States)

    Rene Escobar R.; Manuel Sanchez O.; Guillermo Pereira C.

    2002-01-01

    The forest economy in Chile is based on products from artificial reforestation efforts on approximately 2 million ha. From these, about 1.5 million ha (75%) are planted with Pinus radiata, 400,000 ha (20%) with species of Eucalyptus, principally E. globulus and E. nitens, and the rest (5%) composed by other...

  9. Essential oil composition of Eucalyptus microtheca and Eucalyptus viminalis

    Directory of Open Access Journals (Sweden)

    Malek Taher Maghsoodlou

    2015-10-01

    Full Text Available Objective: Eucalyptus (Fam. Myrtaceae is a medicinal plant and various Eucalyptus species possess potent pharmacological actions against diabetes, hepatotoxicity, and inflammation. This study aims to investigate essential oil compositionfrom leaves and flowers of E. microthecaand E. viminalisleavesgrowing in the Southeast of Iran. Materials and Methods: The aerial parts of these plants were collected from Zahedan, Sistan and Baluchestan province, Iran in 2013.After drying the plant materials in the shade, the chemical composition of the essential oils was obtained by hydro-distillation method using a Clevenger-type apparatus and analyzed by GC/MS. Results: In the essential oil of E. microtheca leaves, 101 compounds representing 100%, were identified. Among them, α-phellandrene (16.487%, aromadendrene (12.773%, α-pinene (6.752%, globulol (5.997%, ledene (5.665%, P-cymen (5.251%, and β-pinene (5.006% were the major constituents.  In the oil of E. microtheca flowers, 88 compounds representing 100%, were identified in which α-pinene (16.246%, O-cymen (13.522%, β-pinene (11.082%, aromadendrene (7.444%, α-phellandrene (7.006%, globulol (5.419%, and 9-octadecenamide (5.414% were the major components. Sixty six compounds representing 100% were identified in the oil of E. viminalis leaves. The major compounds were 1, 8-cineole (57.757%, α-pinene (13.379%, limonene (5.443%, and globulol (3.054%. Conclusion: The results showed the essential oils fromthe aerial parts of Eucalyptus speciesare a cheap source for the commercial isolation of α-phellandrene, α-pinene, and 1, 8-cineole compounds to be used in medicinal and food products. Furthermore, these plants could be an alternative source of insecticide agents.

  10. Essential oil composition of Eucalyptus microtheca and Eucalyptus viminalis.

    Science.gov (United States)

    Maghsoodlou, Malek Taher; Kazemipoor, Nasrin; Valizadeh, Jafar; Falak Nezhad Seifi, Mohsen; Rahneshan, Nahid

    2015-01-01

    Eucalyptus (Fam. Myrtaceae) is a medicinal plant and various Eucalyptus species possess potent pharmacological actions against diabetes, hepatotoxicity, and inflammation. This study aims to investigate essential oil composition from leaves and flowers of E. microtheca and E. viminalis leaves growing in the Southeast of Iran. The aerial parts of these plants were collected from Zahedan, Sistan and Baluchestan province, Iran in 2013. After drying the plant materials in the shade, the chemical composition of the essential oils was obtained by hydro-distillation method using a Clevenger-type apparatus and analyzed by GC/MS. In the essential oil of E. microtheca leaves, 101 compounds representing 100%, were identified. Among them, α-phellandrene (16.487%), aromadendrene (12.773%), α-pinene (6.752%), globulol (5.997%), ledene (5.665%), P-cymen (5.251%), and β-pinene (5.006%) were the major constituents. In the oil of E. microtheca flowers, 88 compounds representing 100%, were identified in which α-pinene (16.246%), O-cymen (13.522%), β-pinene (11.082%), aromadendrene (7.444%), α-phellandrene (7.006%), globulol (5.419%), and 9-octadecenamide (5.414%) were the major components. Sixty six compounds representing 100% were identified in the oil of E. viminalis leaves. The major compounds were 1, 8-cineole (57.757%), α-pinene (13.379%), limonene (5.443%), and globulol (3.054%). The results showed the essential oils from the aerial parts of Eucalyptus species are a cheap source for the commercial isolation of α-phellandrene, α-pinene, and 1, 8-cineole compounds to be used in medicinal and food products. Furthermore, these plants could be an alternative source of insecticide agents.

  11. Aggregate stability in soils cultivated with eucalyptus

    Science.gov (United States)

    Eucalyptus cultivation has increased in many Brazilian regions. In order to recommend good management practices, it is necessary to understand changes in soil properties where eucalyptus is planted. Aggregate stability analyses have proved to be a useful tool to measure soil effects caused by change...

  12. Quantification and economic valuation of the capture of CO2 for Eucalyptus plantations, established by the Preca in the carboniferous Basins of Cesar, Cauca Valley, Cauca and Highland Cundiboyacense

    International Nuclear Information System (INIS)

    Diaz Fonseca, Sandra Ximena; Molano Morales, Miguel Angel

    2001-01-01

    This study, as first measure it looks for to quantify the tons of CO 2 captured by the increment in the biomass of forestall plantations of the Eucalyptus genus established by the PRECA of Ecocarbon in the carboniferous basins of the Cesar, Cauca Valley, Cauca and Highland Cundiboyacense and in second measure to determine the economic value that the sale of this environmental service can represent for a developing country as Colombia. The results obtained for each one of the plantations in each carboniferous basin are determined, and statistical models that will allow calculating the capture of CO 2 carried out by plantations of three different species of Eucalyptus (E. camaldulensis, e. grandis and E. globulus), starting from the volume in foot of the timber only barked

  13. Quantification and economic valuation of the capture of CO2 for plantations of the Eucalyptus, genus, settled down by the PRECA in the carboniferous basins of Cesar, Cauca Valley, Cauca and Cundiboyacense Highland

    International Nuclear Information System (INIS)

    Diaz F, Sandra Ximena; Molano M, Miguel Angel

    2001-01-01

    In this study, the first measure is to quantify the tons of CO 2 captured by the increment in the biomass of forest plantations of the Eucalyptus genus, settled down by the PRECA of Ecocarbon in the carboniferous basins of the Cesar, Cauca Valley, Cauca and Cundiboyacense highland and in second measure to determine the economic value that the sale of this environmental service can represent for a developing country as Colombia. The results obtained for each one of the plantations settled down in each carboniferous basin are determined and statistical models that will allow to calculate the capture of CO 2 carried out by plantations of three different species of Eucalyptus (E. Camaldulensis, E. grandis and E. globulus)

  14. ESTUDIOS SOBRE EL COMPORTAMIENTO DE FORRAJEO DE Acromyrmex lundi Guering (HYMENOPTERA, FORMICIDAE Y SU EFECTO SOBRE EL CRECIMIENTO DE PROCEDENCIAS DE Eucalyptus globulus Labill. (Myrtaceae

    Directory of Open Access Journals (Sweden)

    Carmen Lidia Martínez

    2015-02-01

    Full Text Available El ataque de las hormigas cortadoras es una de las principales razones de pérdida de plantas durante la etapa de establecimiento de un monte forestal. Los objetivos de este estudio fueron evaluar la intensidad de forrajeo de Acromyrmex lundi asociado a algunas condiciones ambientales y procedencias de Eucalyptus globulus y estimar el impacto de la herbivoría sobre el crecimiento inicial de estos árboles. Dos ensayos se llevaron a cabo durante dos temporadas estivo-otoñales consecutivas, en el Campo Experimental de la Universidad Nacional de Luján, Argentina. Los materiales de diferentes procedencias fueron dispuestos en bloques equidistantes a 5 m de un nido activo de A. lundi. Durante doce semanas se contabilizó el número de hojas cortadas por las hormigas y se registraron algunas variables meteorológicas. En el segundo año la mitad de las parcelas fue protegida de las hormigas y se midieron variables de crecimiento. Los materiales de todas las procedencias fueron atacadas por A. lundi, aunque el número de hojas cosechada fue significativamente diferente sólo entre los orígenes Flinders Island (52,5 hojas y Nullo Mountain (28,5 hojas. El número de días lluviosos fue el único factor ambiental que se relacionó positivamente con la actividad de las hormigas.Todos los tratamientos redujeron significativamente la altura, el diámetro y el área foliar cuando fueron atacados por las hormigas. Las distintas procedencias compensaron diferencialemnte la herbivoría. Se discuten los resultados en función de los criterios a tener en cuenta para la selección de materiales genéticos como herramienta de manejo y control del ataque de A. lundi.

  15. Heavy metal pathways and archives in biological tissue

    Energy Technology Data Exchange (ETDEWEB)

    Orlic, I. E-mail: ivo@ansto.gov.au; Siegele, R.; Menon, D.D.; Markich, S.J.; Cohen, D.D.; Jeffree, R.A.; McPhail, D.C.; Sarbutt, A.; Stelcer, E

    2002-05-01

    Nuclear milli and microprobes at the Australian Nuclear Science and Technology Organisation (ANSTO) were used to determine lead accumulation in native Australian plants and animals. Three species of eucalypt plants (Eucalyptus camaldulensis, Eucalyptus globulus and Eucalyptus lesouefii), one species of salt bush (Atriplex burbhanyana) and one species each of acacia (Acacia saligna) and estuarine crocodiles (Crocodylus porosus) were investigated. Experimentally grown plants were subjected to a nutrient solution with a pH of 5 and spiked with a 200 {mu}mol concentration of Pb. Lead concentrations in leaves of both E. globulus and E. camaldulensis showed an almost exponential decrease from the base of the main vein to the tip. Similarly, Pb concentrations decreased from the main vein to secondary veins. Concentrations of essential elements such as K, Fe, Zn and Br in the main and secondary veins were constant within experimental uncertainty. In contrast, the concentrations of Pb in the leaf veins of E. lesouefii were much lower and showed no systematic pattern. In stem and root samples the highest concentration of Pb was found in roots and stem of E. globulus and A. burbhanyana followed by E. camaldulensis. Some Pb was found in roots of A. saligna and only very low concentration in stem of the same plant. More detailed analysis of thin cross-sectional samples of roots and stem showed that Pb is present in much higher concentration in the growth area of the plant structure (i.e. meristemic region) and in relatively low concentration within the pith region and outer cortex. The osteoderms (dermal bones) of estuarine crocodiles, exposed to lead ammunition in food from the hunting activities of traditional Aboriginal owners, were sampled at two sites in Kakadu National Park, northern Australia. PIXE analyses showed enhanced, but relatively constant, ratios of Pb/Ca in the annual laminations. This was consistent with both their history of long term exposure to elevated

  16. Heavy metal pathways and archives in biological tissue

    International Nuclear Information System (INIS)

    Orlic, I.; Siegele, R.; Menon, D.D.; Markich, S.J.; Cohen, D.D.; Jeffree, R.A.; McPhail, D.C.; Sarbutt, A.; Stelcer, E.

    2002-01-01

    Nuclear milli and microprobes at the Australian Nuclear Science and Technology Organisation (ANSTO) were used to determine lead accumulation in native Australian plants and animals. Three species of eucalypt plants (Eucalyptus camaldulensis, Eucalyptus globulus and Eucalyptus lesouefii), one species of salt bush (Atriplex burbhanyana) and one species each of acacia (Acacia saligna) and estuarine crocodiles (Crocodylus porosus) were investigated. Experimentally grown plants were subjected to a nutrient solution with a pH of 5 and spiked with a 200 μmol concentration of Pb. Lead concentrations in leaves of both E. globulus and E. camaldulensis showed an almost exponential decrease from the base of the main vein to the tip. Similarly, Pb concentrations decreased from the main vein to secondary veins. Concentrations of essential elements such as K, Fe, Zn and Br in the main and secondary veins were constant within experimental uncertainty. In contrast, the concentrations of Pb in the leaf veins of E. lesouefii were much lower and showed no systematic pattern. In stem and root samples the highest concentration of Pb was found in roots and stem of E. globulus and A. burbhanyana followed by E. camaldulensis. Some Pb was found in roots of A. saligna and only very low concentration in stem of the same plant. More detailed analysis of thin cross-sectional samples of roots and stem showed that Pb is present in much higher concentration in the growth area of the plant structure (i.e. meristemic region) and in relatively low concentration within the pith region and outer cortex. The osteoderms (dermal bones) of estuarine crocodiles, exposed to lead ammunition in food from the hunting activities of traditional Aboriginal owners, were sampled at two sites in Kakadu National Park, northern Australia. PIXE analyses showed enhanced, but relatively constant, ratios of Pb/Ca in the annual laminations. This was consistent with both their history of long term exposure to elevated

  17. Growth potential limits drought morphological plasticity in seedlings from six Eucalyptus provenances.

    Science.gov (United States)

    Maseda, Pablo H; Fernández, Roberto J

    2016-02-01

    Water stress modifies plant above- vs belowground biomass allocation, i.e., morphological plasticity. It is known that all species and genotypes reduce their growth rate in response to stress, but in the case of water stress it is unclear whether the magnitude of such reduction is linked to the genotype's growth potential, and whether the reduction can be largely attributed to morphological adjustments such as plant allocation and leaf and root anatomy. We subjected seedlings of six seed sources, three from each of Eucalyptus camaldulensis (potentially fast growing) and E. globulus (inherently slow growing), to three experimental water regimes. Biomass, leaf area and root length were measured in a 6-month glasshouse experiment. We then performed functional growth analysis of relative growth rate (RGR), and aboveground (leaf area ratio (LAR), specific leaf area (SLA) and leaf mass ratio (LMR)) and belowground (root length ratio (RLR), specific root length (SRL) and root mass ratio (RMR)) morphological components. Total biomass, root biomass and leaf area were reduced for all Eucalyptus provenances according to drought intensity. All populations exhibited drought plasticity, while those of greater growth potential (RGRmax) had a larger reduction in growth (discounting the effect of size). A positive correlation was observed between drought sensitivity and RGRmax. Aboveground, drought reduced LAR and LMR; under severe drought a negative correlation was found between LMR and RGRmax. Belowground, drought reduced SRL but increased RMR, resulting in no change in RLR. Under severe drought, a negative correlation was found between RLR, SRL and RGRmax. Our evidence strongly supports the classic ecophysiological trade-off between growth potential and drought tolerance for woody seedlings. It also suggests that slow growers would have a low capacity to adjust their morphology. For shoots, this constraint on plasticity was best observed in partition (i.e., LMR) whereas for

  18. Antioxidant effects and phytochemical analysis of crude and ...

    African Journals Online (AJOL)

    Eucalyptus globulus is extremely used in traditional medicine in Algeria and its leaf extract is included as one of the antioxidant in the list of existing food additives in Japan. In the present study, the crude extract from bark of this plant and its fractions obtained by Sephadex LH-20 column were studied to quantify their levels ...

  19. Post-fire plant diversity and abundance in pine and eucalypt stands in Portugal : Effects of biogeography, topography, forest type and post-fire management

    NARCIS (Netherlands)

    Maia, P.; Keizer, J.; Vasques, A.; Abrantes, N.; Roxo, L.; Fernandes, P.; Ferreira, A.; Moreira, F.

    2014-01-01

    This study concerned the mid-term regeneration of the woody understory vegetation of pure and mixed stands of Pinus pinaster Ait. and Eucalyptus globulus Labill. in northern and central Portugal following wildfires in 2005 and 2006. Pine and eucalypt stands are the most widespread and most

  20. Eucalyptus gunnii: A possible source of bioenergy?

    Energy Technology Data Exchange (ETDEWEB)

    Forrest, Mary; Moore, Tom [UCD School of Biology and Environmental Science, Agriculture and Food Science Centre, University College Dublin, Belfield, Dublin 4 (Ireland)

    2008-10-15

    Eucalyptus are widely grown throughout the world for timber, essential oil, fuel, biomass and cut foliage. In experiments in University College Dublin (UCD), Eucalyptus gunnii yielded an average dry weight of 4.74 kg per plant per annum, equivalent to 12.59 t dm ha{sup -1}. The average calorific values of 17.60 MJ kg{sup -1} (leafy material) and 17.09 MJ kg{sup -1} (woody material) are less than the values reported for other species of Eucalyptus. (author)

  1. Consortium of eucalyptus with forage sorghum in semiarid of Minas Gerais State

    Directory of Open Access Journals (Sweden)

    Carlos Juliano Brant Albuquerque

    2017-11-01

    Full Text Available ABSTRACT: The objective was to estimate the wood yield and essential oil content in three clones of eucalyptus that were planted in four contrasting arrangements and intercropped with sorghum. Eucalyptus clones MA2001 (Eucalyptus camaldulensis x E. tereticornis, A144 (Eucalyptus urophylla x E. grandis, and GG100 (Eucalyptus urophylla x E. grandis, were planted in single rows (10x2m, double rows (2x3+15m and 2x3+20m; and, triple rows (2x3x2+10m in a randomized, complete block design experiment with four replicates. Our results demonstrated that planting spacing did not influence the essential oil yield or diameter at breast height in the clones. However, higher density plantings were shown to result in higher fresh weight of branches and leaves per plant. MA2001 grew taller, produced higher quantity of fresh biomass of branches and leaves per plant and volume of wood per hectare, and yielded more essential oil yield than the other clones. We concluded that MA2001 is the most suitable of the clones tested here for cultivation in water deficit conditions.

  2. Processed eucalyptus trees as a substrate component for greenhouse crop production

    Science.gov (United States)

    Fast growing eucalyptus species are selected for commercial plantings worldwide and are harvested for a variety of uses. Eucalyptus plantings in south Florida are harvested for landscape mulch production, yet this material may have potential as a container substrate for horticulture crop production....

  3. Simulated drift effect of glyphosate in different parts of Eucalyptus grandis plantsEfeito da deriva simulada de glyphosate em diferentes partes da planta de Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Maria Renata Rocha Pereira

    2012-10-01

    Full Text Available The present work aimed to evaluate the effects of simulated drift of glyphoste on Eucalyptus grandis, through the application of low doses in different parts of the plant. The experimental design was a randomized block design with five replications. The treatments were glyphosate application at 0; 30; 60; 90 e 120 g a.e. ha-1 of the commercial formulation Scout®. Three forms of application were used: applying on leaf, on stem, and on the entire plant (leaf + stem. For leaf application, stems were covered with plastic ribbons to protect them from the solution; the same was made with plants that were sprayed on stems, covering leaf with plastic bag. The application was carried out in an armed stationary spray tips XR 11002 VS, with 183 KPa pressure in volume of 200 L ha-1. The eucalyptus plants receiving applications in leaves and whole plant (leaves + stem showing effects of intoxication are more intense about the plants that received the stem applications only. However, there may be increases in height growth and total dry mass of eucalyptus plants in applications of 30 g a.e. ha-1 glyphosate.No presente trabalho objetivou-se avaliar os efeitos da deriva simulada de glyphoste em plantas de Eucalyptus grandis, por meio da aplicação de doses reduzidas em diferentes partes da planta. Utilizouse o delineamento inteiramente casualizado, com cinco repetições. Os tratamentos foram constituídos da aplicação de 0; 30; 60; 90 e 120 g e.a.ha-1 de glyphosate, da formulação comercial Scout®. A aplicação foi realizada de três formas: aplicação sobre as folhas, no caule e na planta inteira (folha + caule. Para a aplicação nas folhas o caule foi coberto com fitas plásticas para evitar que fosse atingido pela solução, e o mesmo foi feito com as plantas que receberam pulverização no caule, cobrindo as folhas com saco plástico. A aplicação foi realizada em um pulverizador estacionário, munido de pontas XR 11002 VS, com pressão de 183

  4. Insecticidal Activity of Some Traditionally Used Ethiopian Medicinal Plants against Sheep Ked Melophagus ovinus

    Directory of Open Access Journals (Sweden)

    Negero Gemeda

    2014-01-01

    Full Text Available Twelve medicinal plants and a commercially used drug Ivermectin were examined for insecticidal activity against Melophagus ovinus sheep ked at different time intervals using in vitro adult immersion test. The findings show that at 3.13 µL/mL, 6.25 µL/mL and 12.5 µL/mL concentration of Cymbopogon citratus, Foeniculum vulgare and Eucalyptus globulus essential oils respectively, recorded 100% mortalities against M. ovinus within 3 hour of exposure. Significantly higher insecticidal activity of essential oils was recorded (P=0.00 when compared to 10 μg/mL Ivermectin after 3-hour exposure of M. ovinus at a concentration of ≥1.57 μL/mL, ≥3 μL/mL, and ≥12.7 μL/mL essential oils of C. citratus, F. vulgare, and E. globulus, respectively. Among essential oils, C. citratus has showed superior potency at a three-hour exposure of the parasite (P=0.00 at a concentration of ≥0.78 μL/mL. Strong antiparasitic activity was recorded by aqueous extract of Calpurnia aurea (80% mortality at a concentration of 200 mg/mL within 24 h among aqueous extracts of 9 medicinal plants. The results indicated all the four medicinal plants, particularly those tested essential oils, can be considered as potential candidates for biocontrol of M. ovinus sheep ked.

  5. Insecticidal Activity of Some Traditionally Used Ethiopian Medicinal Plants against Sheep Ked Melophagus ovinus.

    Science.gov (United States)

    Gemeda, Negero; Mokonnen, Walelegn; Lemma, Hirut; Tadele, Ashenif; Urga, Kelbessa; Addis, Getachew; Debella, Asfaw; Getachew, Mesaye; Teka, Frehiwot; Yirsaw, Kidist; Mudie, Kissi; Gebre, Solomon

    2014-01-01

    Twelve medicinal plants and a commercially used drug Ivermectin were examined for insecticidal activity against Melophagus ovinus sheep ked at different time intervals using in vitro adult immersion test. The findings show that at 3.13 µL/mL, 6.25 µL/mL and 12.5 µL/mL concentration of Cymbopogon citratus, Foeniculum vulgare and Eucalyptus globulus essential oils respectively, recorded 100% mortalities against M. ovinus within 3 hour of exposure. Significantly higher insecticidal activity of essential oils was recorded (P = 0.00) when compared to 10  μ g/mL Ivermectin after 3-hour exposure of M. ovinus at a concentration of ≥1.57  μ L/mL, ≥3  μ L/mL, and ≥12.7  μ L/mL essential oils of C. citratus, F. vulgare, and E. globulus, respectively. Among essential oils, C. citratus has showed superior potency at a three-hour exposure of the parasite (P = 0.00) at a concentration of ≥0.78  μ L/mL. Strong antiparasitic activity was recorded by aqueous extract of Calpurnia aurea (80% mortality) at a concentration of 200 mg/mL within 24 h among aqueous extracts of 9 medicinal plants. The results indicated all the four medicinal plants, particularly those tested essential oils, can be considered as potential candidates for biocontrol of M. ovinus sheep ked.

  6. Modelos de predição para sobrevivência de plantas de Eucalyptus grandis Prediction models of Eucalyptus grandis plant survival

    Directory of Open Access Journals (Sweden)

    Telde Natel Custódio

    2009-01-01

    Full Text Available Objetivou-se com este trabalho comparar modelos de predição de plantas sobreviventes de Eucalyptus grandis. Utilizaram-se os seguintes modelos: modelo linear misto com os dados transformados, utilizando-se as transformações angular e BOX-COX; modelo linear generalizado misto com distribuição binomial e funções de ligação logística, probit e complemento log-log; modelo linear generalizado misto com distribuição Poisson e função de ligação logarítmica. Os dados são provenientes de um experimento em blocos ao acaso, para avaliação de progênies maternas de Eucalyptus grandis, aos 5 anos de idade, em que a variável resposta são plantas sobreviventes. Para comparação dos efeitos entre os modelos foram estimadas as correlações de Spearman e aplicado o teste de permutação de Fisher. Foi possível concluir que, o modelo linear generalizado misto com distribuição Poisson e função de ligação logarítmica se ajustou mal aos dados e que as estimativas para os efeitos fixos e predição para os efeitos aleatórios, não se diferenciaram entre os demais modelos estudados.The objective of this work was to compare models for prediction of the survival of plants of Eucalyptus grandis. The following models were used: linear mixed model with the transformed data, by utilizing the angular transformations and BOX-COX; generalized linear mixed model with binomial distribution and logistic functions, probit and complement log-log links; generalized linear mixed model with Poisson distribution and logarithmic link function. The data came from a randomized block experiment for evaluation of Eucalyptus grandis maternal progenies at five years old, in which the variable response are surviving plants. For comparison of the effects among the models the correlations of Spearman were estimated and the test of permutation of Fisher was applied. It was possible to conclude that: the generalized linear mixed model with Poisson distribution and

  7. Genetic basis of resistance in eucalyptus spp. pathosystems

    Science.gov (United States)

    Acelino Couto Alfenas; Lucio Mauro da Silva Guimaraes; Marcos Deon Vilela Resende

    2012-01-01

    Eucalyptus is the most widely planted hardwood crop in world-wide tropical and subtropical regions because of its high growth rate, broad adaptability, and multipurpose wood properties. Until the 1970s, the Eucalyptus plantations in Brazil were practically disease free. However, plantations have continued to expand into warmer...

  8. Oligo-carrageenan kappa-induced reducing redox status and increase in TRR/TRX activities promote activation and reprogramming of terpenoid metabolism in Eucalyptus trees.

    Science.gov (United States)

    González, Alberto; Gutiérrez-Cutiño, Marlen; Moenne, Alejandra

    2014-06-05

    In order to analyze whether the reducing redox status and activation of thioredoxin reductase (TRR)/thioredoxin(TRX) system induced by oligo-carrageenan (OC) kappa in Eucalyptus globulus activate secondary metabolism increasing terpenoid synthesis, trees were sprayed on the leaves with water, with OC kappa, or with inhibitors of NAD(P)H, ascorbate (ASC) and (GSH) synthesis and TRR activity, CHS-828, lycorine, buthionine sulfoximine (BSO) and auranofine, respectively, and with OC kappa and cultivated for four months. The main terpenoids in control Eucalyptus trees were eucalyptol (76%), α-pinene (7.4%), aromadendrene (3.6%), silvestrene (2.8%), sabinene (2%) and α-terpineol (0.9%). Treated trees showed a 22% increase in total essential oils as well as a decrease in eucalyptol (65%) and sabinene (0.8%) and an increase in aromadendrene (5%), silvestrene (7.8%) and other ten terpenoids. In addition, treated Eucalyptus showed seven de novo synthesized terpenoids corresponding to carene, α-terpinene, α-fenchene, γ-maaliene, spathulenol and α-camphenolic aldehyde. Most increased and de novo synthesized terpenoids have potential insecticidal and antimicrobial activities. Trees treated with CHS-828, lycorine, BSO and auranofine and with OC kappa showed an inhibition of increased and de novo synthesized terpenoids. Thus, OC kappa-induced reducing redox status and activation of TRR/TRX system enhance secondary metabolism increasing the synthesis of terpenoids and reprogramming of terpenoid metabolism in Eucalyptus trees.

  9. Oligo-Carrageenan Kappa-Induced Reducing Redox Status and Increase in TRR/TRX Activities Promote Activation and Reprogramming of Terpenoid Metabolism in Eucalyptus Trees

    Directory of Open Access Journals (Sweden)

    Alberto González

    2014-06-01

    Full Text Available In order to analyze whether the reducing redox status and activation of thioredoxin reductase (TRR/thioredoxin(TRX system induced by oligo-carrageenan (OC kappa in Eucalyptus globulus activate secondary metabolism increasing terpenoid synthesis, trees were sprayed on the leaves with water, with OC kappa, or with inhibitors of NAD(PH, ascorbate (ASC and (GSH synthesis and TRR activity, CHS-828, lycorine, buthionine sulfoximine (BSO and auranofine, respectively, and with OC kappa and cultivated for four months. The main terpenoids in control Eucalyptus trees were eucalyptol (76%, α-pinene (7.4%, aromadendrene (3.6%, silvestrene (2.8%, sabinene (2% and α-terpineol (0.9%. Treated trees showed a 22% increase in total essential oils as well as a decrease in eucalyptol (65% and sabinene (0.8% and an increase in aromadendrene (5%, silvestrene (7.8% and other ten terpenoids. In addition, treated Eucalyptus showed seven de novo synthesized terpenoids corresponding to carene, α-terpinene, α-fenchene, γ-maaliene, spathulenol and α-camphenolic aldehyde. Most increased and de novo synthesized terpenoids have potential insecticidal and antimicrobial activities. Trees treated with CHS-828, lycorine, BSO and auranofine and with OC kappa showed an inhibition of increased and de novo synthesized terpenoids. Thus, OC kappa-induced reducing redox status and activation of TRR/TRX system enhance secondary metabolism increasing the synthesis of terpenoids and reprogramming of terpenoid metabolism in Eucalyptus trees.

  10. Regeneration and transformation of Eucalyptus grandis

    OpenAIRE

    Esteki, Leila

    2013-01-01

    Dissertação de mestrado em Biologia Molecular, Biotecnologia e Bioempreendedorismo em Plantas Eucalyptus grandis is the most widely used species in planted forests in tropical and subtropical areas. The traits of interest underlying Eucalyptus breeding programs concern productivity and wood quality for the pulp and paper industry, as well as biotic and abiotic stress tolerance. The development of an efficient transformation protocol is necessary to explore eucalypt resources th...

  11. Occurrence of proscopiidae in Eucalyptus spp.: analysis of the infestations and spatial distribution

    Directory of Open Access Journals (Sweden)

    Alexandre dos Santos

    2014-12-01

    Full Text Available The objective of this research was to report the occurrence of proscopiidae and to quantify its infestation and spatial distribution in one experimental plantation of eucalypt clones. The study was conducted in an experimental field with eucalypt hybrids of Eucalyptus urophylla x Eucalyptus grandis (clones GG100, Eucalyptus urophylla x Eucalyptus grandis (H13 and of Eucalyptus urophylla x Eucalyptus camaldulensis (VM01. The plantation was established in three continuous blocks with 512 plants with 3 m x 2 m, in Cáceres municipality, Mato Grosso State, Brazil. Infestation of proscopiidae insects was detected two months after planting . The insects were collected for identification. The attacked trees were geo-referenced and the injuries were quantified. The spatial pattern of proscopiidae he attack was determinated using the location information of the damaged plants, which have been submitted to a quadrats analyses using Morisita and binomial dispersion indexes. All the collected insects have been identified as Tetanorhynchus smithi Rehn, 1904 (Orthoptera, Proscopiidae. The infestation differed significantly among the three eucalypts clones and aggregated spatial pattern of attack was observed. This is the first report of this species of proscopiidae in eucalyptus in Mato Grosso State.

  12. Diseases on Eucalyptus species in Zimbabwean plantations and ...

    African Journals Online (AJOL)

    Zimbabwe embarked on planting Eucalyptus species in the early 1900s. Based on a robust breeding programme, it has become a major source of seed for other countries in and outside Africa. Tree health surveys conducted on Eucalyptus in some east and southern African countries over the past two decades have ...

  13. Comparative Genomics Analyses Reveal Extensive Chromosome Colinearity and Novel Quantitative Trait Loci in Eucalyptus

    Science.gov (United States)

    Weng, Qijie; Li, Mei; Yu, Xiaoli; Guo, Yong; Wang, Yu; Zhang, Xiaohong; Gan, Siming

    2015-01-01

    Dense genetic maps, along with quantitative trait loci (QTLs) detected on such maps, are powerful tools for genomics and molecular breeding studies. In the important woody genus Eucalyptus, the recent release of E. grandis genome sequence allows for sequence-based genomic comparison and searching for positional candidate genes within QTL regions. Here, dense genetic maps were constructed for E. urophylla and E. tereticornis using genomic simple sequence repeats (SSR), expressed sequence tag (EST) derived SSR, EST-derived cleaved amplified polymorphic sequence (EST-CAPS), and diversity arrays technology (DArT) markers. The E. urophylla and E. tereticornis maps comprised 700 and 585 markers across 11 linkage groups, totaling at 1,208.2 and 1,241.4 cM in length, respectively. Extensive synteny and colinearity were observed as compared to three earlier DArT-based eucalypt maps (two maps with E. grandis × E. urophylla and one map of E. globulus) and with the E. grandis genome sequence. Fifty-three QTLs for growth (10–56 months of age) and wood density (56 months) were identified in 22 discrete regions on both maps, in which only one colocalizaiton was found between growth and wood density. Novel QTLs were revealed as compared with those previously detected on DArT-based maps for similar ages in Eucalyptus. Eleven to 585 positional candidate genes were obained for a 56-month-old QTL through aligning QTL confidence interval with the E. grandis genome. These results will assist in comparative genomics studies, targeted gene characterization, and marker-assisted selection in Eucalyptus and the related taxa. PMID:26695430

  14. Comparative Genomics Analyses Reveal Extensive Chromosome Colinearity and Novel Quantitative Trait Loci in Eucalyptus.

    Directory of Open Access Journals (Sweden)

    Fagen Li

    Full Text Available Dense genetic maps, along with quantitative trait loci (QTLs detected on such maps, are powerful tools for genomics and molecular breeding studies. In the important woody genus Eucalyptus, the recent release of E. grandis genome sequence allows for sequence-based genomic comparison and searching for positional candidate genes within QTL regions. Here, dense genetic maps were constructed for E. urophylla and E. tereticornis using genomic simple sequence repeats (SSR, expressed sequence tag (EST derived SSR, EST-derived cleaved amplified polymorphic sequence (EST-CAPS, and diversity arrays technology (DArT markers. The E. urophylla and E. tereticornis maps comprised 700 and 585 markers across 11 linkage groups, totaling at 1,208.2 and 1,241.4 cM in length, respectively. Extensive synteny and colinearity were observed as compared to three earlier DArT-based eucalypt maps (two maps with E. grandis × E. urophylla and one map of E. globulus and with the E. grandis genome sequence. Fifty-three QTLs for growth (10-56 months of age and wood density (56 months were identified in 22 discrete regions on both maps, in which only one colocalizaiton was found between growth and wood density. Novel QTLs were revealed as compared with those previously detected on DArT-based maps for similar ages in Eucalyptus. Eleven to 585 positional candidate genes were obained for a 56-month-old QTL through aligning QTL confidence interval with the E. grandis genome. These results will assist in comparative genomics studies, targeted gene characterization, and marker-assisted selection in Eucalyptus and the related taxa.

  15. Production and development of eucalyptus seedlings in function of doses of phosphorus

    Directory of Open Access Journals (Sweden)

    José Henrique Tertulino Rocha

    2013-12-01

    Full Text Available This study evaluated the effect of phosphorus (P on the survival in the nursery and early development in the field of clonal Eucalyptus urophylla x Eucalyptus grandis seedlings. The experimental design was completely randomized with four treatments and five replicates of 30 plants per replicate. At the end of the cycle (90 days, 15 seedlings were used for determining the dry matter accumulation and nutrient concentration in the shoot. The rest was planted in the field to determine the percentage of survival and early development. The treatments consisted of four doses of P fertilization (0.0, 1.3, 2.6 and 5.2 mg plant-1. To obtain high quality seedlings in conditions similar to this experiment it is required doses of P in the range from 3.6 to 3.8 mg plant-1. Doses greater than 4 mg plant-1 affect the development and quality of eucalyptus seedlings. An adequate phosphorus fertilization of eucalyptus seedlings increase, by about 30%, the percentage of surviving seedlings in the field.

  16. BASIC DENSITY AND RETRACTIBILITY OF WOOD CLONES OF THREE Eucalyptus SPECIES

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    Djeison Cesar Batista

    2010-12-01

    Full Text Available Among the planted forests that supply the national wood industry, the genus Eucalyptus has become the most important, due to its fast growth, ease of large scale planting and variability of wood use. The generation of new hybrids and clones is a reality in the national practice of silviculture, and there is great interest currently in finding genetic improvements, mainly for higher volumetric gains and resistance in rough conditions of planting, such as pest attacks, periods of drought, low soil fertility, etc. The basic density is one of the most important physical properties of wood because it relates directly to other properties, including the anisotropic shrinkage. Such properties indicate the rational use of a species in a certain wood product. The aim of this work was to determine the basic density and the anisotropic shrinkage of five wood clones for each one of the following species: Eucalyptus saligna, Eucalyptus grandis and Eucalyptus dunnii. Clone 5 of Eucalyptus saligna presented the highest basic density (0.56 g/cm³ and was the most dimensionally instable. Of all the species, there was only a direct relation among basic density, maximum volumetric shrinkage and maximum volumetric shrinkage coefficient in this clone. Considering maximum volumetric shrinkage as the criterion, clone 3 was the most dimensionally stable. Clones 2 and 3 of Eucalyptus grandis presented the least and the highest basic density, respectively, with 0.40 and 0.49 g/cm³. It was not possible to distinguish among clones 1, 3 and 4 in terms of dimensional stability, and considering maximum volumetric shrinkage coefficient as the criterion, clone 5 was the most dimensionally instable. For Eucalyptus saligna and Eucalyptus dunnii it was not possible to distinguish which clone presented the least basic density. Clone 3 of Eucalyptus dunnii presented the highest basic density (0.65 g/cm³ and considering maximum volumetric shrinkage coefficient as the criterion, it

  17. Fumigant activity of plant essential oils and components from horseradish (Armoracia rusticana), anise (Pimpinella anisum) and garlic (Allium sativum) oils against Lycoriella ingenua (Diptera: Sciaridae).

    Science.gov (United States)

    Park, Ii-Kwon; Choi, Kwang-Sik; Kim, Do-Hyung; Choi, In-Ho; Kim, Lee-Sun; Bak, Won-Chull; Choi, Joon-Weon; Shin, Sang-Chul

    2006-08-01

    Plant essential oils from 40 plant species were tested for their insecticidal activities against larvae of Lycoriella ingénue (Dufour) using a fumigation bioassay. Good insecticidal activity against larvae of L. ingenua was achieved with essential oils of Chenopodium ambrosioides L., Eucalyptus globulus Labill, Eucalyptus smithii RT Baker, horseradish, anise and garlic at 10 and 5 microL L(-1) air. Horseradish, anise and garlic oils showed the most potent insecticidal activities among the plant essential oils. At 1.25 microL L(-1), horseradish, anise and garlic oils caused 100, 93.3 and 13.3% mortality, but at 0.625 microL L(-1) air this decreased to 3.3, 0 and 0% respectively. Analysis by gas chromatography-mass spectrometry led to the identification of one major compound from horseradish, and three each from anise and garlic oils. These seven compounds and m-anisaldehyde and o-anisaldehyde, two positional isomers of p-anisaldehyde, were tested individually for their insecticidal activities against larvae of L. ingenua. Allyl isothiocyanate was the most toxic, followed by trans-anethole, diallyl disulfide and p-anisaldehyde with LC(50) values of 0.15, 0.20, 0.87 and 1.47 microL L(-1) respectively.

  18. Neuroprotective and Anti-Aging Potentials of Essential Oils from Aromatic and Medicinal Plants

    OpenAIRE

    Ayaz, Muhammad; Sadiq, Abdul; Junaid, Muhammad; Ullah, Farhat; Subhan, Fazal; Ahmed, Jawad

    2017-01-01

    The use of essential oils (EOs) and their components is known since long in traditional medicine and aromatherapy for the management of various diseases, and is further increased in the recent times. The neuroprotective and anti-aging potentials of EOs and their possible mechanism of actions were evaluated by numerous researchers around the globe. Several clinically important EOs and their components from Nigella sativa, Acorus gramineus, Lavandula angustifolia, Eucalyptus globulus, Mentha pi...

  19. Phylogenetic reassessment of Mycosphaerella spp. and their anamorphs occurring on Eucalyptus

    NARCIS (Netherlands)

    Crous, P.W.; Groenewald, J.Z.; Mansilla, J.P.; Hunter, G.C.; Wingfield, M.J.

    2004-01-01

    Species of Eucalyptus, mostly native to Australia, are widely planted as exotics in the tropics and Southern Hemisphere. These plantations represent an important source of fuel-wood, structural timber and pulp. Eucalyptus plantations are, however, vulnerable to infection by pathogens, including

  20. WEED CONTROL AND BORON NUTRITION ON Eucalyptus IN SILVOPASTORAL SYSTEM

    Directory of Open Access Journals (Sweden)

    Alexandre Magno Brighenti

    2015-04-01

    Full Text Available The objective of this study was to evaluate the control of weeds in the rows of eucalyptus (Eucalyptus urograndis with herbicides applied singly or combined with boron (B, as well as, the response of Eucalyptus plants to this micronutrient. The experiments were carried out in a split-plot with randomized complete block design, with three replicates. Six treatments were applied to the plots: (i weeded control, (ii control without weeding, (iii glyphosate (1080 g ae ha-1 + chlorimuron-ethyl (10 g ai ha-1 + 0.05% v / v mineral oil, (iv glyphosate (1080 g ae  ha-1 + isoxaflutole (112.5 g ai ha-1,  (v glyphosate (1080 g ae ha-1 and  (vi oxyfluorfen (480 g ai ha-1. The sub-plots consisted of the absence or presence of 4 kg of boric acid (H3BO3 - 17% B in 100 L of water. The addition of boric acid in the solution containing the herbicides did not affect the weed control. There was an increase in boron content in the soil and consequently an increase in the boron levels in the eucalyptus leaves. The combined application of herbicides plus boric acid is perfectly suitable for preventing dry of the pointer on eucalyptus plants. Keywords: Eucalyptus urograndis; chemical control; herbicides; micronutrients; Urochloa decumbens.

  1. on black ironbark (Eucalyptus

    African Journals Online (AJOL)

    (Apis meOifera) on black ironbark. (Eucalyptus sideroxylon). B. Buys. Plant Protection Research Institute, Private Bag X5017,. Stellenbosch, 7600 Republic of South Africa. Received May /984; accepted 28 November /986. Black ironbark trees secrete nectar during the night. Argentine ants collected 42% of the nectar before ...

  2. Potencial de onze espécies de eucalipto para a produção de óleos essenciais

    OpenAIRE

    Silva, Paulo Henrique Müller da; Brito, José Otávio; Silva Junior, Francides Gomes da

    2006-01-01

    Most Eucalyptus plantations in Brazil aim the production of paper and charcoal, but the use of the species for lumbering, construction and extraction of essential oil has increased. Eleven species of Eucalyptus were assessed in regard to their essential oil production potential, nine never used before for commercial, essential oil extraction. Assessements were compared with Eucalyptus citriodora and Eucalyptus globulus, already explored in oil production for perfume and medical purposes, aimi...

  3. Biomass in monospecific and mixed stands of eucalyptus and black wattle and corn in an agroforestry system

    Directory of Open Access Journals (Sweden)

    Márcio Viera

    2011-06-01

    Full Text Available This study aimed at quantifying the production and distribution of aboveground biomass from the plants in monospecific and mixed stands of eucalyptus (hybrid E. urophylla x E. grandis and black wattle (Acacia mearnsii and, of corn (Zea mays in agrosilvicultural systems. The biomass evaluation (leaf, branch, bark and wood from the forest species at 6 and 18 months of age were performed at the treatments: 100E (100% of eucalyptus + corn; - 100A (100% of black wattle + corn; - 50E:50A (50% of eucalyptus + 50% of black wattle + corn. The corn biomass evaluation (stem, leaves, straw, cob and grains was performed at treatments 100E; 100A; 50E:50A; 75E:25A (75% of eucalyptus + 25% of black wattle + corn; and - 25E:75A (25% of eucalyptus + 75% of black wattle + corn. The biomass production from eucalyptus and from the black wattle, in both monospecific and mixed planting, did not differ in any of the assessed ages but, when evaluated by plants compartments, it was verified an interspecific competitive interaction from the eucalyptus on the black wattle, reducing the formation of crown biomass. The total production of corn biomass in agrosilvicutural systems with eucalyptus and with black wattle in monospecific or mixed plantings did not differ in the studied treatments.

  4. Fertilization value of municipal sewage sludge for Eucalyptus camaldulensis plants

    Directory of Open Access Journals (Sweden)

    Soudani Leila

    2017-03-01

    Full Text Available The wastewater treatment produces a large amount of sludge. The different uses of eliminations sludge such as landfills or incineration have consequences negative for the environment, the agricultural use has increased worldwide, especially in crops and few or no studies have been conducted with forest plantations in Algeria. The objective of this study is to assess fertilizing characteristics of the sludge from the wastewater treatment plant of Tiaret (Algeria. One-year-old saplings of Eucalyptus camaldulensis were transplanted into pots with sludge/soil mixtures where sludge content was 20%, 40% and 60%. Biometric measurements (height, base diameter, diameter at mid-height and the number of leaves were performed during six months after planting. Results demonstrated the positive effect of sludge application. A significant difference in height increment and number of leaves was found between the control and sludge-treated plants. Biometric values for all sludge mixtures were higher than those for control plants (100% soil. The mixture, which contained 60% sludge, gives the best result, except for a diameter of stem. Plants grown on sludge/soil mixture had average height 49.4 ± 24.1 cm and average number of leaves 68.8 ± 6.2 while average height for plants grown on soil was 34.3 ± 12.8 cm and average number of leaves was 40 ± 3.8. Sludge application provides soil amendment and additional nutrient supply for planted trees.

  5. Potential of eleven Eucalyptus species for the production of essential oils Potencial de onze espécies de eucalipto para a produção de óleos essenciais

    Directory of Open Access Journals (Sweden)

    Paulo Henrique Müller da Silva

    2006-02-01

    Full Text Available Most Eucalyptus plantations in Brazil aim the production of paper and charcoal, but the use of the species for lumbering, construction and extraction of essential oil has increased. Eleven species of Eucalyptus were assessed in regard to their essential oil production potential, nine never used before for commercial, essential oil extraction. Assessements were compared with Eucalyptus citriodora and Eucalyptus globulus, already explored in oil production for perfume and medical purposes, aiming to increase the availability of commercial species and the relationship between oil production and climatic conditions. Eucalyptus leaves were seasonally sampled (three months interval for distillation and determination of productivity and chemical composition of oil - contents of oils main components. Eucalyptus viminalis showed the highest potential for cineol, and standed out among the species not yet used commercially. Influenced by soil moisture contents and air temperature, the lowest and the highest oil production happened respectively in Spring and Summer. Water deficiency in Spring caused the lowest oil production. In the Summer, on the other hand, the highest oil production was observed when temperatures were higher and no water deficiency registered. There was no climatic influence on the main oil chemical component.A maioria das plantações de Eucalyptus brasileiras tem como objetivo a produção de papel e carvão, mas o uso das espécies para madeira, construção e extração de óleo essencial tem aumentado. Neste trabalho foram estudadas onze espécies de eucalipto quanto ao potencial para a obtenção de óleo essencial. Entre as espécies estudadas, nove não têm sido utilizadas na obtenção comercial de óleo. Para efeito comparativo, avaliou-se também duas outras espécies, o Eucalyptus citriodora e o Eucalyptus globulus, as quais são amplamente utilizadas, respectivamente, para a obtenção de óleo aromatizante e óleo para

  6. Eucalyptus beyond its native range: Environmental issues in exotic bioenergy plantations

    Science.gov (United States)

    John A. Stanturf; Eric D. Vance; Thomas R. Fox; Matias Kirst

    2013-01-01

    The genus Eucalyptus is native to Australia and Indonesia but has been widely planted in many countries. Eucalyptus has proven to be particularly successful in tropical and subtropical regions. Several species are also successful in some temperate regions, but problems with sudden and severe frosts pose limitations. Current...

  7. Estimating foliar nitrogen in Eucalyptus using vegetation indexes

    Directory of Open Access Journals (Sweden)

    Luiz Felipe Ramalho de Oliveira

    Full Text Available ABSTRACT Nitrogen (N has commonly been applied in Eucalyptus stands in Brazil and it has a direct relation with biomass production and chlorophyll content. Foliar N concentrations are used to diagnose soil and plant fertility levels and to develop N fertilizer application rates. Normally, foliar N is obtained using destructive methods, but indirect analyses using Vegetation Indexes (VIs may be possible. The aim of this work was to evaluate VIs to estimate foliar N concentration in three Eucalyptus clones. Lower crown leaves of three clonal Eucalyptus plantations (25 months old were classified into five color patterns using the Munsell Plant Tissue Color Chart. For each color, N concentration was determined by the Kjeldahl method and foliar reflectance was measured using a CI-710 Miniature Leaf Spectrometer. Foliar reflectance data were used to obtain the VIs and the VIs were used to estimate N concentrations. In the visible region, the relationship between N concentration and reflectance percentage was negative. The highest correlations between VIs and N concentrations were obtained by the Inflection Point Position (IPP, r = 0.97, Normalized Difference Red-Edge (reNDVI, r = 0.97 and Modified Red-Edge Normalized Difference Vegetation Index (mNDI, r = 0.97. Vegetation indexes on the red edge region provided the most accurate estimates of foliar N concentration. The reNDVI index provided the best N concentration estimates in leaves of different colors of Eucalyptus urophylla × grandis and Eucalyptus urophylla × urophylla (R2 = 0.97 and RMSE = 0.91 g kg−1.

  8. Oldest Known Eucalyptus Macrofossils Are from South America

    Science.gov (United States)

    Zamaloa, María C.; Nixon, Kevin C.; González, Cynthia C.; Wilf, Peter; Cúneo, N. Rubén; Johnson, Kirk R.

    2011-01-01

    The evolutionary history of Eucalyptus and the eucalypts, the larger clade of seven genera including Eucalyptus that today have a natural distribution almost exclusively in Australasia, is poorly documented from the fossil record. Little physical evidence exists bearing on the ancient geographical distributions or morphologies of plants within the clade. Herein, we introduce fossil material of Eucalyptus from the early Eocene (ca. 51.9 Ma) Laguna del Hunco paleoflora of Chubut Province, Argentina; specimens include multiple leaves, infructescences, and dispersed capsules, several flower buds, and a single flower. Morphological similarities that relate the fossils to extant eucalypts include leaf shape, venation, and epidermal oil glands; infructescence structure; valvate capsulate fruits; and operculate flower buds. The presence of a staminophore scar on the fruits links them to Eucalyptus, and the presence of a transverse scar on the flower buds indicates a relationship to Eucalyptus subgenus Symphyomyrtus. Phylogenetic analyses of morphological data alone and combined with aligned sequence data from a prior study including 16 extant eucalypts, one outgroup, and a terminal representing the fossils indicate that the fossils are nested within Eucalyptus. These are the only illustrated Eucalyptus fossils that are definitively Eocene in age, and the only conclusively identified extant or fossil eucalypts naturally occurring outside of Australasia and adjacent Mindanao. Thus, these fossils indicate that the evolution of the eucalypt group is not constrained to a single region. Moreover, they strengthen the taxonomic connections between the Laguna del Hunco paleoflora and extant subtropical and tropical Australasia, one of the three major ecologic-geographic elements of the Laguna del Hunco paleoflora. The age and affinities of the fossils also indicate that Eucalyptus subgenus Symphyomyrtus is older than previously supposed. Paleoecological data indicate that the

  9. Oldest known Eucalyptus macrofossils are from South America.

    Directory of Open Access Journals (Sweden)

    María A Gandolfo

    Full Text Available The evolutionary history of Eucalyptus and the eucalypts, the larger clade of seven genera including Eucalyptus that today have a natural distribution almost exclusively in Australasia, is poorly documented from the fossil record. Little physical evidence exists bearing on the ancient geographical distributions or morphologies of plants within the clade. Herein, we introduce fossil material of Eucalyptus from the early Eocene (ca. 51.9 Ma Laguna del Hunco paleoflora of Chubut Province, Argentina; specimens include multiple leaves, infructescences, and dispersed capsules, several flower buds, and a single flower. Morphological similarities that relate the fossils to extant eucalypts include leaf shape, venation, and epidermal oil glands; infructescence structure; valvate capsulate fruits; and operculate flower buds. The presence of a staminophore scar on the fruits links them to Eucalyptus, and the presence of a transverse scar on the flower buds indicates a relationship to Eucalyptus subgenus Symphyomyrtus. Phylogenetic analyses of morphological data alone and combined with aligned sequence data from a prior study including 16 extant eucalypts, one outgroup, and a terminal representing the fossils indicate that the fossils are nested within Eucalyptus. These are the only illustrated Eucalyptus fossils that are definitively Eocene in age, and the only conclusively identified extant or fossil eucalypts naturally occurring outside of Australasia and adjacent Mindanao. Thus, these fossils indicate that the evolution of the eucalypt group is not constrained to a single region. Moreover, they strengthen the taxonomic connections between the Laguna del Hunco paleoflora and extant subtropical and tropical Australasia, one of the three major ecologic-geographic elements of the Laguna del Hunco paleoflora. The age and affinities of the fossils also indicate that Eucalyptus subgenus Symphyomyrtus is older than previously supposed. Paleoecological data

  10. Energy-Based Evaluations on Eucalyptus Biomass Production

    Directory of Open Access Journals (Sweden)

    Thiago L. Romanelli

    2012-01-01

    Full Text Available Dependence on finite resources brings economic, social, and environmental concerns. Planted forests are a biomass alternative to the exploitation of natural forests. In the exploitation of the planted forests, planning and management are key to achieve success, so in forestry operations, both economic and noneconomic factors must be considered. This study aimed to compare eucalyptus biomass production through energy embodiment of anthropogenic inputs and resource embodiment including environmental contribution (emergy for the commercial forest in the Sao Paulo, Brazil. Energy analyses and emergy synthesis were accomplished for the eucalyptus production cycles. It was determined that emergy synthesis of eucalyptus production and sensibility analysis for three scenarios to adjust soil acidity (lime, ash, and sludge. For both, energy analysis and emergy synthesis, harvesting presented the highest input demand. Results show the differences between energy analysis and emergy synthesis are in the conceptual underpinnings and accounting procedures. Both evaluations present similar trends and differ in the magnitude of the participation of an input due to its origin. For instance, inputs extracted from ores, which represent environmental contribution, are more relevant for emergy synthesis. On the other hand, inputs from industrial processes are more important for energy analysis.

  11. CARACTERIZACION DE RIZOBACTERIAS PROMOTORAS DEL ENRAIZAMIENTO DE ESTACAS DE EUCALYPTUS GLOBULUS LABILL. : MECANISMOS DE ACCION Y FLUCTUACION POBLACIONAL

    OpenAIRE

    DIAZ PERALTA, KATY PAULINA; DIAZ PERALTA, KATY PAULINA

    2009-01-01

    Las plantaciones de Eucalyptus spp en Chile, son altamente valoradas en el mercado mundial, su producción se basa en la propagación vegetativa de plantas en viveros, a través de estacas y miniestacas; sin embargo, ex iste una variabilidad en el potencial del enraizamiento entre clones de especies e híbridos del género, lo cual no permite obtener el total de plantas en los programas de producción clonal. Diversos estudios han demostrado que bacterias rizosféricas denominadas bacterias promotor...

  12. Isotopic abundance of 13 C and contribution of eucalyptus biomass to soil organic matter conversion

    Directory of Open Access Journals (Sweden)

    Fabiane Figueiredo Severo

    Full Text Available ABSTRACT: It has become possible to evaluate the conversion of soil organic matter (SOM in pastures and arboreal crops due to the difference between the photosynthetic cycles of Eucalyptus (C3 and most grasses (C4. The auto analyzer method coupled to the IRMS (Isotope Ratio Mass Spectrometer in the present study evaluated the 13C content in soil profiles of Eucalyptus plantations of different ages (2, 10 and 21 years, in natural regeneration areas and natural grazing fields, and estimated the SOM conversion of each crop type of. The initial management of all sampled areas was natural pasture. The following profile layers were evaluated: 0-5, 5-10, 10-20, 20-30, 30-40, 40-50, 50-70 and 70-90cm, and the contribution of Eucalyptus biomass over the years of farming was estimated in the SOM conversion process. After 2 years of planting Eucalyptus, the beginning of pasture carbon conversion process occurred in the surface layer (0-5cm. Ten years after planting, the process of converting organic matter by arboreal crops reached the layers up to 20cm. After 21 years of planting and in natural regeneration areas, the entire profile has already been changed by planting Eucalyptus and native tree species.

  13. Complex linkage between soil, soil water, atmosphere and Eucalyptus Plantations

    Science.gov (United States)

    Shukla, C.; Tiwari, K. N.

    2017-12-01

    Eucalyptus is most widely planted genus grown in waste land of eastern region of India to meet the pulp industry requirements. Sustainability of these plantations is of concern because in spite of higher demand water and nutrients of plantations, they are mostly planted on low-fertility soils. This study has been conducted to quantify effect of 25 years old, a fully established eucalyptus plantations on i.) Alteration in physico-chemical and hydrological properties of soil of eucalyptus plantation in comparison to soil of natural grassland and ii.) Spatio-temporal variation in soil moisture under eucalyptus plantations. Soil physico-chemical properties of two adjacent plots covered with eucatuptus and natural grasses were analyzed for three consecutive depths (i.e. 0-30 cm, 30-60 cm and 60-90 cm) with five replications in each plot. Soil infiltration rate and saturated hydraulic conductivity (Ks) were measured in-situ to incorporate the influence of macro porosity caused due to roots of plantations. Daily soil moisture at an interval of 10 cm upto 160 cm depth with 3 replications and Leaf Area Index (LAI) at an interval of 15 days with 5 replications were recorded over the year. Significant variations found at level of 0.05 between soil properties of eucalyptus and natural grass land confirm the effect of plantations on soil properties. Comparative results of soil properties show significant alteration in soil texture such as percent of sand, organic matter and Ks found more by 20%, 9% and 22% respectively in eucalyptus plot as compare to natural grass land. Available soil moisture (ASM) was found constantly minimum in top soil excluding rainy season indicate upward movement of water and nutrients during dry season. Seasonal variation in temperature (T), relative humidity (RH) and leaf area index (LAI) influenced the soil moisture extraction phenomenon. This study clearly stated the impact of long term establishment of eucalyptus plantations make considerable

  14. First insights into the functional role of vasicentric tracheids and parenchyma in eucalyptus species with solitary vessels: do they contribute to xylem efficiency or safety?

    Science.gov (United States)

    Barotto, Antonio José; Fernandez, María Elena; Gyenge, Javier; Meyra, Ariel; Martinez-Meier, Alejandro; Monteoliva, Silvia

    2016-12-01

    The relationship between hydraulic specific conductivity (k s ) and vulnerability to cavitation (VC) with size and number of vessels has been studied in many angiosperms. However, few of the studies link other cell types (vasicentric tracheids (VT), fibre-tracheids, parenchyma) with these hydraulic functions. Eucalyptus is one of the most important genera in forestry worldwide. It exhibits a complex wood anatomy, with solitary vessels surrounded by VT and parenchyma, which could serve as a good model to investigate the functional role of the different cell types in xylem functioning. Wood anatomy (several traits of vessels, VT, fibres and parenchyma) in conjunction with maximum k s and VC was studied in adult trees of commercial species with medium-to-high wood density (Eucalyptus globulus Labill., Eucalyptus viminalis Labill. and Eucalyptus camaldulensis Dehnh.). Traits of cells accompanying vessels presented correlations with functional variables suggesting that they contribute to both increasing connectivity between adjacent vessels-and, therefore, to xylem conduction efficiency-and decreasing the probability of embolism propagation into the tissue, i.e., xylem safety. All three species presented moderate-to-high resistance to cavitation (mean P 50 values = -2.4 to -4.2 MPa) with no general trade-off between efficiency and safety at the interspecific level. The results in these species do not support some well-established hypotheses of the functional meaning of wood anatomy. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  15. Estimations of evapotranspiration in an age sequence of Eucalyptus plantations in subtropical China.

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    Wenfei Liu

    Full Text Available Eucalyptus species are widely planted for reforestation in subtropical China. However, the effects of Eucalyptus plantations on the regional water use remain poorly understood. In an age sequence of 2-, 4- and 6-year-old Eucalyptus plantations, the tree water use and soil evaporation were examined by linking model estimations and field observations. Results showed that annual evapotranspiration of each age sequence Eucalyptus plantations was 876.7, 944.1 and 1000.7 mm, respectively, accounting for 49.81%, 53.64% and 56.86% of the annual rainfall. In addition, annual soil evaporations of 2-, 4- and 6-year-old were 318.6, 336.1, and 248.7 mm of the respective Eucalyptus plantations. Our results demonstrated that Eucalyptus plantations would potentially reduce water availability due to high evapotranspiration in subtropical regions. Sustainable management strategies should be implemented to reduce water consumption in Eucalyptus plantations in the context of future climate change scenarios such as drought and warming.

  16. Preferência de corte de Acromyrmex crassispinus (Forel, 1909) e Acromyrmex ambiguus (Emery, 1887) (Hymenoptera: Formicidae) por diferentes espécies de eucaliptos em laboratório

    OpenAIRE

    Rosado, João Luis Osório; Loeck, Alci Enimar; Freitas, Deise Farias; Gonçalves, Michel Gonçalves de; Dröse, William; Cunha, Uemerson Silva da; Finkenauer, Elder

    2014-01-01

    A busca por novos métodos de manejo e controle de formigas-cortadeiras vem sendo enfoque de inúmeros estudos pelo país, impulsionada por aspectos econômicos e ambientais. O estabelecimento de espécies resistentes ou menos preferidas ao corte pode contribuir substancialmente neste sentido. Logo, o objetivo deste estudo foi avaliar a preferência de corte entre as espécies Corymbia citriodora, Eucalyptus benthamii, Eucalyptus camaldulensis, Eucalyptus dunnii, Eucalyptus globulus e híbrido de Euc...

  17. In silico characterization of microsatellites in Eucalyptus spp.: abundance, length variation and transposon associations

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    Edenilson Rabello

    2005-01-01

    Full Text Available This study assessed the abundance of microsatellites, or simple sequence repeats (SSR, in 19 Eucalyptus EST libraries from FORESTs, containing cDNA sequences from five species: E. grandis, E. globulus, E. saligna, E. urophylla and E. camaldulensis. Overall, a total of 11,534 SSRs and 8,447 SSR-containing sequences (25.5% of total ESTs were identified, with an average of 1 SSR/2.5 kb when considering all motifs and 1 SSR/3.1 kb when mononucleotides were not included. Dimeric repeats were the most abundant (41.03%, followed by trimerics (36.11% and monomerics (19.59%. The most frequent motifs were A/T (87.24% for monomerics, AG/CT (94.44% for dimerics, CCG/CGG (37.87% for trimerics, AAGG/CCTT (18.75% for tetramerics, AGAGG/CCTCT (14.04% for pentamerics and ACGGCG/CGCCGT (6.30% for hexamerics. According to sequence length, Class II or potentially variable markers were the most commonly found, followed by Class III. Two sequences presented high similarity to previously published Eucalyptus sequences from the NCBI database, EMBRA_72 and EMBRA_122. Local blastn search for transposons did not reveal the presence of any transposable elements with a cut-off value of 10-50. The large number of microsatellites identified will contribute to the refinement of marker-assisted mapping and to the discovery of novel markers for virtually all genes of economic interest.

  18. USE OF TREND IMPACT ANALYSIS AFFECTS PROJECTIONS OF EUCALYPTUS CULTIVATION IN BRAZIL

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    Samir Lotfi

    2009-10-01

    Full Text Available Eucalyptus forestry is an important source of competitive advantage for Brazil and, since eucalyptus is a basic raw material for industrial segments that are undergoing great expansion, monitoring the growth rate of cultivated area is increasingly relevant.This study resorted to Trend Impact Analysis (TIA to forecast the planting of eucalyptus in Brazil’s reforested areas, adjusting the linear regression of historical data in the light of three events that were under way or that will probably influence the trend: timber funds, the world financial crisis and Biomass to Liquid (BTL technology.The results allow one to infer that, in the short term, eucalyptus cultivation will expand at a rate similar to that of the linear curve, adversely affected by the world crisis and positively affected by timber funds.By 2016, however, the expansion of eucalyptus plantations is expected, largely because of the commercial scale of BTL technology.Key words: Trend Impact Analysis. Eucalyptus. Future Studies.

  19. Antifungal activity of lemon, eucalyptus, thyme, oregano, sage and lavender essential oils against Aspergillus niger and Aspergillus tubingensis isolated from grapes

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    Miroslava Císarová

    2016-01-01

    Full Text Available Today, it is very important to find out the protection of products of natural origin as an alternative to synthetic fungicides. The promising alternative is the use of the essential oils (EOs. Essential oils from plants have great potential as a new source of fungicide to control the pathogenic fungi.The main objective of this study was evaluation of the antifungal activity of lemon (Citrus lemon L., eucalyptus (Eucalyptus globulus LABILL., thyme (Thymus vulgaris L., oregano (Origanum vulgare L. sage (Salvia officinalis L. and lavender (Lavandula angustifolia MILLER. EOs against Aspergillus niger and Aspergillus tubingensis isolated from grapes and their ability to affect the growth. It was tested by using the vapor contact with them. At first both tested isolates were identified by using PCR method. Sequence data of 18S rRNA supported the assignment of these isolates to the genus Aspergillus and species A. niger (ITS region: KT824061; RPB2: KT824060 and A. tubingensis (ITS region: KT824062; RPB2: KT824059. Second, EO antifungal activity was evaluated. The effect of the EO volatile phase was confirmed to inhibit growth of A. niger and A tubingensis. EOs were diluted in DMSO (dimethyl sulfoxide final volume of 100 μL. Only 50 μL this solution was distributed on a round sterile filter paper (1 x 1 cm by micropipette, and the paper was placed in the center of the lid of Petri dishes. Dishes were kept in an inverted position. The essential oils with the most significant activity were determined by method of graded concentration of oils - minimum inhibitory doses (MIDs. The most effective tested EOs were oregano and thyme oils, which totally inhibited growth of tested isolates for all days of incubation at 0.625 μL.cm-3 (in air with MFDs 0.125 μL.cm-3 (in air. Lavender EO was less active aginst tested strains (MIDs 0.313 μL.cm-3. The results showed that the tested EOs had antifungal activity, except lemon and eucalyptus. Sage EO was the only

  20. BVOC emissions from 2 Asian Eucalyptus species,E.camadulensis and E.robusta

    Science.gov (United States)

    Tsui, J.; Guenther, A. B.; Chan, C. K.; Lau, A. P.

    2009-12-01

    Eucalyptus species dominate native forests in Australia and are planted over vast regions in Asia and other continents for afforestation and for pulp due to their fast growth rates. However, they have also been identified as high emitters of biogenic volatile organic compounds (BVOCs). BVOCs, when emitted to the atmosphere, react to form air pollutants such as ozone (O3) and secondary organic aerosols (SOA). The large areas of Eucalyptus forests in Australia and Asia, and high BVOC emission rates of Eucalyptus species, imply a potential significant effect of BVOC emissions from Eucalyptus on the air quality of these regions. A better understanding of BVOC emissions from this genus is thus needed. Here we present data of BVOC measurements from E.camadulensis and E.robusta. BVOC emissions of the 2 Eucalyptus species were measured by a branch enclosure approach in an environmental chamber, in which light and temperature were carefully controlled to mimic their changes throughout the day under natural conditions. E. camadulensis was found to emit isoprene, α-pinene, camphene and limonene, while E. robusta was found to emit isoprene, α-pinene, β-pinene, α-phellandrene, 3-carene and ocimene. Diurnal variations in BVOC emissions from the 2 species were observed. The 2 Eucalyptus species were also treated with methyl jasmonate (MeJA), a plant hormone which has found to induce elevated BVOC emissions similar to response to insect attacks in other plant species. The emission profiles of the 2 species before and after MeJA treatment were contrasted to examine the effects of the MeJA on their BVOC emissions. Acknowledgment: This work was supported by the General Research Fund of the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. 610909).

  1. Combining autohydrolysis and ionic liquid microwave treatment to enhance enzymatic hydrolysis of Eucalyptus globulus wood.

    Science.gov (United States)

    Rigual, Victoria; Santos, Tamara M; Domínguez, Juan Carlos; Alonso, M Virginia; Oliet, Mercedes; Rodriguez, Francisco

    2018-03-01

    The combination of autohydrolysis and ionic liquid microwave treatments of eucalyptus wood have been studied to facilitate sugar production in a subsequent enzymatic hydrolysis step. Three autohydrolysis conditions (150 °C, 175 °C and 200 °C) in combination with two ionic liquid temperatures (80 °C and 120 °C) were compared in terms of chemical composition, enzymatic digestibility and sugar production. Morphology was measured (using SEM) and the biomass surface was visualized with confocal fluorescence microscopy. The synergistic cooperation of both treatments was demonstrated, enhancing cellulose accessibility. At intermediate autohydrolysis conditions (175 °C) and low ionic liquid temperature (80 °C), a glucan digestibility of 84.4% was obtained. Using SEM micrographs, fractal dimension (as a measure of biomass complexity) and lacunarity (as a measure of homogeneity) were calculated before and after pretreatment. High fractals dimensions and low lacunarities correspond to morphologically complex and homogeneous samples, that are better digested by enzyme cocktails. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Evaluation of gamma irradiated essential oils to control stored-grain weevil, Sitophilus zeamais and cockroach, Blattella germanica

    International Nuclear Information System (INIS)

    Potenza, Marcos Roberto; Gomes, Davinni Habral Passini; Silvestre, Denise de Fatima; Da Silva, Rita de Cassia; Arthur, Valter

    2004-01-01

    Most of the essential oils have insecticidal activity and are alternatives to plant protection and urban pest control. The gamma radiation has been used to control microorganisms and insects in dehydrate herbs, spices, medicinal plants and other materials. This work aimed to evaluate the effect of irradiated essential oils as contact poison on Stored grain, weevil, Sitophilus zeamais and as stomach poison in cockroach, Blattella germanica. The bioassay with B. germanica demonstrated efficiency between 22.0 and 30.0% and between 30.0 and 42.0% respectively, to irradiated essential oils of E. citriodora and E. globulus and they demonstrated too repellency to the nymphs. The gamma radiation promoted changes in essential oils Eucalyptus citriodora and E. globulus and showed increased effectiveness on B. germanica nymphs besides a significative reduction of repellency. Gamma radiation thus interfered on behavior of some essential oils by increasing or reducing activating or inactivating their efficiency to pest control. (author)

  3. Environmental effects on growth phenology of co-occurring Eucalyptus species.

    Science.gov (United States)

    Rawal, Deepa S; Kasel, Sabine; Keatley, Marie R; Aponte, Cristina; Nitschke, Craig R

    2014-05-01

    Growth is one of the most important phenological cycles in a plant's life. Higher growth rates increase the competitive ability, survival and recruitment and can provide a measure of a plant's adaptive capacity to climate variability and change. This study identified the growth relationship of six Eucalyptus species to variations in temperature, soil moisture availability, photoperiod length and air humidity over 12 months. The six species represent two naturally co-occurring groups of three species each representing warm-dry and the cool-moist sclerophyll forests, respectively. Warm-dry eucalypts were found to be more tolerant of higher temperatures and lower air humidity than the cool-moist eucalypts. Within groups, species-specific responses were detected with Eucalyptus microcarpa having the widest phenological niche of the warm-dry species, exhibiting greater resistance to high temperature and lower air humidity. Temperature dependent photoperiodic responses were exhibited by all the species except Eucalyptus tricarpa and Eucalyptus sieberi, which were able to maintain growth as photoperiod shortened but temperature requirements were fulfilled. Eucalyptus obliqua exhibited a flexible growth rate and tolerance to moisture limitation which enables it to maintain its growth rate as water availability changes. The wider temperature niche exhibited by E. sieberi compared with E. obliqua and Eucalyptus radiata may improve its competitive ability over these species where winters are warm and moisture does not limit growth. With climate change expected to result in warmer and drier conditions in south-east Australia, the findings of this study suggest all cool-moist species will likely suffer negative effects on growth while the warm-dry species may still maintain current growth rates. Our findings highlight that climate driven shifts in growth phenology will likely occur as climate changes and this may facilitate changes in tree communities by altering inter

  4. DENSIDADE BÁSICA E RETRATIBILIDADE DA MADEIRA DE CLONES DE TRÊS ESPÉCIES DE Eucalyptus

    Directory of Open Access Journals (Sweden)

    Djeison Cesar Batista

    2010-01-01

    Full Text Available Among the planted forests that supply the national wood industry, the genus Eucalyptus has become the most important, due to its fast growth, ease of large scale planting and variability of wood use. The generation of new hybrids and clones is a reality in the national practice of silviculture, and there is great interest currently in finding genetic improvements, mainly for higher volumetric gains and resistance in rough conditions of planting, such as pest attacks, periods of drought, low soil fertility, etc. The basic density is one of the most important physical properties of wood because it relates directly to other properties, including the anisotropic shrinkage. Such properties indicate the rational use of a species in a certain wood product. The aim of this work was to determine the basic density and the anisotropic shrinkage of five wood clones for each one of the following species: Eucalyptus saligna, Eucalyptus grandis and Eucalyptus dunnii. Clone 5 of Eucalyptus saligna presented the highest basic density (0.56 g/cm³ and was the most dimensionally instable. Of all the species, there was only a direct relation among basic density, maximum volumetric shrinkage and maximum volumetric shrinkage coefficient in this clone. Considering maximum volumetric shrinkage as the criterion, clone 3 was the most dimensionally stable. Clones 2 and 3 of Eucalyptus grandis presented the least and the highest basic density, respectively, with 0.40 and 0.49 g/cm³. It was not possible to distinguish among clones 1, 3 and 4 in terms of dimensional stability, and considering maximum volumetric shrinkage coefficient as the criterion, clone 5 was the most dimensionally instable. For Eucalyptus saligna and Eucalyptus dunnii it was not possible to distinguish which clone presented the least basic density. Clone 3 of Eucalyptus dunnii presented the highest basic density (0.65 g/cm³ and considering maximum volumetric shrinkage coefficient as the criterion, it

  5. Thermodynamic Study of Adsorption of Phenol, 4-Chlorophenol, and 4-Nitrophenol on Activated Carbon Obtained from Eucalyptus Seed

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    Nelson Giovanny Rincón-Silva

    2015-01-01

    Full Text Available Activated carbons from shell eucalyptus (Eucalyptus globulus were prepared by chemical activation through impregnation with solutions of two activators: sulfuric acid and sodium hydroxide, the surface areas for activated carbons with base were 780 and 670 m2 g−1 and the solids activated with acid were 150 and 80 m2 g−1. These were applying in adsorption of priority pollutants: phenol, 4-nitrophenol, and 4-chlorophenol from aqueous solution. Activated carbon with the highest adsorption capacity has values of 2.12, 2.57, and 3.89 on phenol, 4-nitrophenol, and 4-chlorophenol, respectively, and was activated with base. In general, all carbons adsorption capacity was given in the following order: 4-chlorophenol > 4-nitrophenol > phenol. Adsorption isotherms of phenols on activated carbons were fitted to the Langmuir, Freundlich, and Dubinin-Radusckevisch-Kanager models, finding great association between them and experimental data. A thermodynamic study was performed, the exothermic nature and spontaneous nature of the adsorption process were confirmed, and the favorability of adsorption on activated carbons with NaOH was confirmed by energy relations and concluded that the adsorption process of phenolic compounds from the activated carbon obtained is physical. The pH of solutions and pH at point of zero charge of the solid play an important role in the adsorption process.

  6. Water erosion risk prediction in eucalyptus plantations

    Directory of Open Access Journals (Sweden)

    Mayesse Aparecida da Silva

    2014-04-01

    Full Text Available Eucalyptus plantations are normally found in vulnerable ecosystems such as steep slope, soil with low natural fertility and lands that were degraded by agriculture. The objective of this study was to obtain Universal Soil Loss Equation (USLE factors and use them to estimate water erosion risk in regions with eucalyptus planted. The USLE factors were obtained in field plots under natural rainfall in the Rio Doce Basin, MG, Brazil, and the model applied to assess erosion risk using USLE in a Geographic Information System. The study area showed rainfall-runoff erosivity values from 10,721 to 10,642 MJ mm ha-1 h-1 yr-1. Some soils (Latosols had very low erodibility values (2.0 x 10-4 and 1.0 x 10-4t h MJ-1 mm-1, the topographic factor ranged from 0.03 to 10.57 and crop and management factor values obtained for native forest, eucalyptus and planted pasture were 0.09, 0.12 and 0.22, respectively. Water erosion risk estimates for current land use indicated that the areas where should receive more attention were mainly areas with greater topographic factors and those with Cambisols. Planning of forestry activities in this region should consider implementation of other conservation practices beyond those already used, reducing areas with a greater risk of soil erosion and increasing areas with very low risk.

  7. Foliar Essential Oil Glands of Eucalyptus Subgenus Eucalyptus (Myrtaceae Are a Rich Source of Flavonoids and Related Non-Volatile Constituents.

    Directory of Open Access Journals (Sweden)

    Jason Q D Goodger

    Full Text Available The sub-dermal secretory cavities (glands embedded within the leaves of Eucalyptus (Myrtaceae were once thought to be the exclusive repositories of monoterpene and sesquiterpene oils. Recent research has debunked this theory and shown that abundant non-volatile compounds also occur within foliar glands. In particular, glands of four species in subgenus Eucalyptus contain the biologically active flavanone pinocembrin. Pinocembrin shows great promise as a pharmaceutical and is predominantly plant-sourced, so Eucalyptus could be a potential commercial source of such compounds. To explore this we quantified and assessed the purity of pinocembrin in glands of 11 species of E. subg. Eucalyptus using Electro-Spray Ionisation Liquid Chromatography Mass Spectrometry of acetonitrile extracts and Gas Chromatography Mass Spectrometry analyses of hexane extracts of isolated glands which were free from other leaf tissues. Our results showed that the glands of subgenus Eucalyptus contain numerous flavanones that are structurally related to pinocembrin and often present in much greater abundance. The maximum concentration of pinocembrin was 2 mg g-1 dry leaf found in E. stellulata, whereas that of dimethylpinocembrin (5,7-dimethoxyflavanone was 10 mg g-1 in E. oreades and that of pinostrobin (5-hydroxy-7-methoxyflavanone was 12 mg g-1 in E. nitida. We also found that the flavanones are exclusively located within the foliar glands rather than distributed throughout leaf tissues. The flavanones differ from the non-methylated pinocembrin in the degree and positions of methylation. This finding is particularly important given the attractiveness of methylated flavonoids as pharmaceuticals and therapeutics. Another important finding was that glands of some members of the subgenus also contain flavanone O-glucosides and flavanone-β-triketone conjugates. In addition, glands contain free β-triketones, β-triketone heterodimers and chromone C-glucosides. Therefore, the

  8. Foliar Essential Oil Glands of Eucalyptus Subgenus Eucalyptus (Myrtaceae) Are a Rich Source of Flavonoids and Related Non-Volatile Constituents.

    Science.gov (United States)

    Goodger, Jason Q D; Seneratne, Samiddhi L; Nicolle, Dean; Woodrow, Ian E

    2016-01-01

    The sub-dermal secretory cavities (glands) embedded within the leaves of Eucalyptus (Myrtaceae) were once thought to be the exclusive repositories of monoterpene and sesquiterpene oils. Recent research has debunked this theory and shown that abundant non-volatile compounds also occur within foliar glands. In particular, glands of four species in subgenus Eucalyptus contain the biologically active flavanone pinocembrin. Pinocembrin shows great promise as a pharmaceutical and is predominantly plant-sourced, so Eucalyptus could be a potential commercial source of such compounds. To explore this we quantified and assessed the purity of pinocembrin in glands of 11 species of E. subg. Eucalyptus using Electro-Spray Ionisation Liquid Chromatography Mass Spectrometry of acetonitrile extracts and Gas Chromatography Mass Spectrometry analyses of hexane extracts of isolated glands which were free from other leaf tissues. Our results showed that the glands of subgenus Eucalyptus contain numerous flavanones that are structurally related to pinocembrin and often present in much greater abundance. The maximum concentration of pinocembrin was 2 mg g-1 dry leaf found in E. stellulata, whereas that of dimethylpinocembrin (5,7-dimethoxyflavanone) was 10 mg g-1 in E. oreades and that of pinostrobin (5-hydroxy-7-methoxyflavanone) was 12 mg g-1 in E. nitida. We also found that the flavanones are exclusively located within the foliar glands rather than distributed throughout leaf tissues. The flavanones differ from the non-methylated pinocembrin in the degree and positions of methylation. This finding is particularly important given the attractiveness of methylated flavonoids as pharmaceuticals and therapeutics. Another important finding was that glands of some members of the subgenus also contain flavanone O-glucosides and flavanone-β-triketone conjugates. In addition, glands contain free β-triketones, β-triketone heterodimers and chromone C-glucosides. Therefore, the foliar glands

  9. Methanol production from Eucalyptus wood chips. Working document I. The Florida Eucalyptus energy farm: silvicultural methods and considerations

    Energy Technology Data Exchange (ETDEWEB)

    Fishkind, H.H.

    1982-04-01

    The silvicultural matrix within which the nation's first large scale wood energy plantation will develop is described in detail. The relevant literature reviewed is identified and distilled. The plantation history, site preparation, planting, species selection, maintenance and management, harvesting, and the Eucalyptus biomass production estimates are presented.

  10. History and Status of Eucalyptus Improvement in Florida

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    Donald L. Rockwood

    2012-01-01

    Full Text Available The first organized Eucalyptus research in Florida was begun by the Florida Forests Foundation in 1959 in southern Florida. This research was absorbed by the USDA Forest Service and the Florida Division of Forestry in 1968. In the early 1970s, the Eucalyptus Research Cooperative formed to provide additional support emphasized E. grandis, E. robusta, E. camaldulensis, and E. tereticornis and developed cultural practices for commercial plantations in southern Florida. In 1978, this cooperative united with the Hardwood Research Cooperative at North Carolina State University until 1985 when the 14-year effort ended after three severe freezes from 1983 to 1985. Eucalyptus planting and research were continued with a Florida-wide focus by the University of Florida and collaborators starting in 1980. The collective accomplishments in terms of genetic resources and commercial planting are summarized. For example, fast-growing, freeze-resilient E. grandis seedlings are produced by advanced generation seed orchards, five E. grandis cultivars are commercially available, as are E. amplifolia and Corymbia torelliana seeds. Genetic improvement of these and other species is ongoing due to beneficial collaborations. Short Rotation Woody Crop systems are promising for increasing productivity and extending uses beyond conventional pulpwood to applications such as windbreaks, dendroremediation, and energy wood.

  11. Eucalyptus tolerance mechanisms to lanthanum and cerium: subcellular distribution, antioxidant system and thiol pools.

    Science.gov (United States)

    Shen, Yichang; Zhang, Shirong; Li, Sen; Xu, Xiaoxun; Jia, Yongxia; Gong, Guoshu

    2014-12-01

    Guanglin 9 (Eucalyptus grandis × Eucalyptus urophlla) and Eucalyptus grandis 5 are two eucalyptus species which have been found to grow normally in soils contaminated with lanthanum and cerium, but the tolerance mechanisms are not clear yet. In this study, a pot experiment was conducted to investigate the tolerance mechanisms of the eucalyptus to lanthanum and cerium. Cell walls stored 45.40-63.44% of the metals under lanthanum or cerium stress. Peroxidase and catalase activities enhanced with increasing soil La or Ce concentrations up to 200 mg kg(-1), while there were no obvious changes in glutathione and ascorbate concentrations. Non-protein thiols concentrations increased with increasing treatment levels up to 200 mg kg(-1), and then decreased. Phytochelatins concentrations continued to increase under La or Ce stress. Therefore, the two eucalyptus species are La and Ce tolerant plants, and the tolerance mechanisms include cell wall deposition, antioxidant system response, and thiol compound synthesis. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Permeability measuremens of brazilian Eucalyptus

    Directory of Open Access Journals (Sweden)

    Marcio Rogério da Silva

    2010-09-01

    Full Text Available The permeability of Brazilian Eucalyptus grandis and Eucalyptus citriodora wood was measured in a custom build gas analysis chamber in order to determine which species could be successfully treated with preservatives. Liquid permeability was tested using an emulsion of Neen oil and a control of distillated water. Air was used to test the gas phase permeability. For both Eucalyptus grandis and Eucalyptus citriodora, the longitudinal permeability of gas was shown to be about twice as great as the liquid phase permeability. No radial permeability was observed for either wood. The permeability of air and water through the sapwood of Eucalyptus grandis was greater than that through the sapwood of Eucalyptus citriodora. The permeability of neen oil preservative through the sapwood of Eucalyptus grandis was also greater than through the sapwood of E. Citradora, but the difference was not statistically significant. Scanning Electron Microscopy images showed that the distribution and obstruction in the vessels could be correlated with observed permeability properties. Irrespective of the causes of differences in permeability between the species, the fluid phase flux through the sapwood of both species was significant, indicating that both Eucalyptus grandis and Eucalyptus citriodora could be successfully treated with wood preservative.

  13. Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis Regeneração de plantas de Eucalyptus camaldulensis a partir das explantes cotiledonares

    Directory of Open Access Journals (Sweden)

    Roberson Dibax

    2005-08-01

    Full Text Available Breeding methods based on genetic transformation techniques need to be implemented for Eucalyptus camaldulensis to shorten the long breeding cycles and avoid manipulation of adult trees; that requires the development of plant regeneration protocols enabling development of plants from transformed tissues. The present work aimed to optimise the regeneration process already established for the species. Cotyledonary leaves of E. camaldulensis were cultured in MS medium supplemented with naphthaleneacetic acid (NAA and 6-benzylaminopurine (BAP combinations. The most efficient treatment for bud indirect regeneration (2.7 µmol L-1 NAA and 4.44 µmol L-1 BAP was used for further experiments. When explants were kept in the dark during the first 30 days, the percentage of explants forming calluses increased and explant necrosis was reduced in comparison with light-cultured explants. Mineral medium modifications were compared and half-strength MS mineral medium turned out to be as efficient as full-strength medium, producing 54% and 47% of explants with buds, respectively. For shoot elongation, MS medium with half-strength nitrate and ammonium salts, and 0.2% activated charcoal yielded rooted shoots 1 to 8 cm high after one month. The procedure is an efficient protocol for E. camadulensis plant regeneration, reducing the stages necessary for the obtention of complete plants.A implementação, para espécies florestais, de técnicas de melhoramento baseadas em métodos de transformação genética, permitirá reduzir os longos ciclos de melhoramento e evitar a manipulação de árvores adultas. Isto implica dispor de um protocolo de regeneração que permita o desenvolvimento de plantas a partir de tecidos transformados. Este trabalho teve como objetivo otimizar este protocolo de regeneração para Eucalyptus camaldulensis. Folhas cotiledonares foram cultivadas em meio de cultura MS suplementado com combinações de ácido naftalenoacético (ANA e 6

  14. Water relations of Eucalyptus nitens x Eucalyptus grandis : is there ...

    African Journals Online (AJOL)

    Water relations of Eucalyptus nitens x Eucalyptus grandis : is there interclonal variation in response to experimentally imposed water stress? ... Southern Forests: a Journal of Forest Science ... However, water stress reduced shoot hydraulic conductance and stem hydraulic conductivity with significant interclonal effects.

  15. Uji Infeksi Mycosphaerella spp Terhadap Bibit Eucalyptus spp

    OpenAIRE

    Lidya Morita Sondang

    2009-01-01

    Tujuan penelitian ini adalah untuk mengetahui tingkat ketahanan 2 klon Eucalyptus spp yaitu Eucalyptus grandis x Eucalyptus pellita dan Eucalyptus grandis x Eucalyptus urophylla terhadap Mycosphaerella spp serta mengetahui virulensi Mycospaherella spp pada 2 kelas umur (2 dan 3 bulan) pada tanaman Eucalyptus spp. Penelitian ini dilaksanakan dengan pengambilan sampel bibit tanaman Eucalyptus grandis x Eucalyptus pellita dan Eucalyptus grandis x Eucalyptus urophylla dari pembibitan PT.Toba Pulp...

  16. Eucalyptus oil poisoning.

    OpenAIRE

    Patel, S; Wiggins, J

    1980-01-01

    Accidental ingestion of eucalyptus oil by a 3-year-old boy caused profound central nervous system depression within 30 minutes, but he recovered rapidly after gastric lavage. The extreme toxicity of eucalyptus oil is emphasised.

  17. Is Eucalyptus Cryptically Self-incompatible?

    Science.gov (United States)

    Horsley, Tasmien N; Johnson, Steven D

    2007-12-01

    The probability that seeds will be fertilized from self- versus cross-pollen depends strongly on whether plants have self-incompatibility systems, and how these systems influence the fate of pollen tubes. In this study of breeding systems in Eucalyptus urophylla and Eucalyptus grandis, epifluorescence microscopy was used to study pollen tube growth in styles following self- and cross-pollinations. Pollen tubes from self-pollen took significantly longer than those from cross-pollen to grow to the base of the style in both E. urophylla (120 h vs. 96 h) and E. grandis (96 h vs. 72 h). In addition, both species exhibited reduced seed yields following self-pollination compared with cross-pollination. The present observations suggest that, in addition to a late-acting self-incompatibility barrier, cryptic self-incompatibility could be a mechanism responsible for the preferential out-crossing system in these two eucalypt species.

  18. Estrogenicity and intersex in juvenile rainbow trout (Oncorhynchus mykiss) exposed to Pine/Eucalyptus pulp and paper production effluent in Chile

    Energy Technology Data Exchange (ETDEWEB)

    Chiang, Gustavo, E-mail: gchiang@centromeri.cl [Canadian Rivers Institute and Department of Biology, University of New Brunswick, Saint John, NB E2L 4L5 (Canada); Barra, Ricardo [Aquatic Systems Research Unit, EULA–Chile Environmental Sciences Centre, University of Concepción, Concepcion (Chile); Díaz-Jaramillo, Mauricio [Laboratorio de Ecotoxicología y Contaminación Ambiental (ECoA), IIMyC-CONICET-UNMdP, Mar del Plata (Argentina); Rivas, Meyling [Department of Zoology, Faculty of Natural and Oceanographic Sciences, University of Concepcion, Concepcion (Chile); Bahamonde, Paulina; Munkittrick, Kelly R. [Canadian Rivers Institute and Department of Biology, University of New Brunswick, Saint John, NB E2L 4L5 (Canada)

    2015-07-15

    Highlights: • Juvenile rainbow were exposed to Pine and Eucalyptus PPME along with an in situ bioassay downstream of the combined discharge. • Fish exposed to PPME showed induced levels of plasma vitellogenin and female gonad maturation. • Male fish showed intersex characteristics in laboratory and in situ assays. • Tertiary treated PPME from Eucalyptus production have stronger estrogenic effects on juvenile fish. - Abstract: Pulp and paper mill effluents (PPMEs) have been shown to increase gonad size, cause early maturation, and disrupt hormone functions in native and non-native Chilean fish. In this study, we assessed reproductive (plasma vitellogenin; VTG, gonad development) and metabolic (ethoxyresorufin-O-deethylase activity; EROD) end points, relative liver size (LSI) and condition factor (K) of juvenile female and male rainbow trout exposed to effluents. Unlike previous studies, which have focus either on the specific effects of effluent on fish in laboratory exposures or biotic population statuses downstream of discharge sites, we simultaneously assessed the impacts of PPMES on trout using two approaches: (1) laboratory exposures of tertiary treated PPME produced from processing Eucalyptus globulus or Pinus radiata; and (2) in situ bioassay downstream of the combined discharge of the same pulp mill. Despite an increase in the average gonadosomatic index (GSI) in exposed fish, no statistical differences in gonad size between exposed and unexposed individuals was detected. However, both female and male fish exposed to effluents showed significantly higher concentrations of plasma VTG, so more in fish exposed to Eucalyptus-based effluent when compared to Pinus PPME. In addition, male fish showed intersex characteristics in all exposure assays (Eucaliptus and Pinus) and, despite the low concentration of effluent in the river (<1% [v/v]), similar responses were observed in the caged fish. Finally, EROD activity was induced in both in situ exposures and

  19. Estrogenicity and intersex in juvenile rainbow trout (Oncorhynchus mykiss) exposed to Pine/Eucalyptus pulp and paper production effluent in Chile

    International Nuclear Information System (INIS)

    Chiang, Gustavo; Barra, Ricardo; Díaz-Jaramillo, Mauricio; Rivas, Meyling; Bahamonde, Paulina; Munkittrick, Kelly R.

    2015-01-01

    Highlights: • Juvenile rainbow were exposed to Pine and Eucalyptus PPME along with an in situ bioassay downstream of the combined discharge. • Fish exposed to PPME showed induced levels of plasma vitellogenin and female gonad maturation. • Male fish showed intersex characteristics in laboratory and in situ assays. • Tertiary treated PPME from Eucalyptus production have stronger estrogenic effects on juvenile fish. - Abstract: Pulp and paper mill effluents (PPMEs) have been shown to increase gonad size, cause early maturation, and disrupt hormone functions in native and non-native Chilean fish. In this study, we assessed reproductive (plasma vitellogenin; VTG, gonad development) and metabolic (ethoxyresorufin-O-deethylase activity; EROD) end points, relative liver size (LSI) and condition factor (K) of juvenile female and male rainbow trout exposed to effluents. Unlike previous studies, which have focus either on the specific effects of effluent on fish in laboratory exposures or biotic population statuses downstream of discharge sites, we simultaneously assessed the impacts of PPMES on trout using two approaches: (1) laboratory exposures of tertiary treated PPME produced from processing Eucalyptus globulus or Pinus radiata; and (2) in situ bioassay downstream of the combined discharge of the same pulp mill. Despite an increase in the average gonadosomatic index (GSI) in exposed fish, no statistical differences in gonad size between exposed and unexposed individuals was detected. However, both female and male fish exposed to effluents showed significantly higher concentrations of plasma VTG, so more in fish exposed to Eucalyptus-based effluent when compared to Pinus PPME. In addition, male fish showed intersex characteristics in all exposure assays (Eucaliptus and Pinus) and, despite the low concentration of effluent in the river (<1% [v/v]), similar responses were observed in the caged fish. Finally, EROD activity was induced in both in situ exposures and

  20. Temporal dynamics of the response to Al stress in Eucalyptus grandis × Eucalyptus camaldulensis

    Directory of Open Access Journals (Sweden)

    Berenice K. de Alcântara

    2015-06-01

    Full Text Available Lipid peroxidation and root elongation of Eucalyptus grandis × Eucalyptus camaldulensis were studied under stress conditions in response to aluminum (Al, a metal known to limit agricultural productivity in acidic soils primarily due to reduced root elongation. In Brazil, the Grancam 1277 hybrid (E. grandis × E. camaldulensis has been planted in the "Cerrado", a region of the country with a wide occurrence of acidic soils. The present study demonstrated that the hybrid exhibited root growth reduction and increased levels of lipid peroxidation after 24h of treatment with 100 µM of Al, which was followed by a reduction in lipid peroxidation levels and the recovery of root elongation after 48h of Al exposure, suggesting a rapid response to the early stressful conditions induced by Al. The understanding of the temporal dynamics of Al tolerance may be useful for selecting more tolerant genotypes and for identifying genes of interest for applications in bioengineering.

  1. Managing a Coastal Bluegum (Eucalyptus globules) forest

    Science.gov (United States)

    Ralph S. Osterling

    1983-01-01

    Eucalyptus was thought to be a replacement to oak and other hardwoods for many products. Thousands of acres have been planted and now are in need of management. Management techniques are discussed in context with a coastal stand of approximately 300 acres of mixed aged bluegum. Potential markets are explored.

  2. Antihyperglycemic effect of crude extracts of some Egyptian plants and algae.

    Science.gov (United States)

    AbouZid, Sameh Fekry; Ahmed, Osama Mohamed; Ahmed, Rasha Rashad; Mahmoud, Ayman; Abdella, Ehab; Ashour, Mohamed Badr

    2014-03-01

    Diabetes mellitus is a major global health problem. Various plant extracts have proven antidiabetic activity and are considered as promising substitution for antidiabetic drugs. The antihyperglycemic effect of 16 plants and 4 algae, commonly used in Egypt for the treatment of diabetes mellitus, was investigated. A diabetes model was induced by intraperitoneal injection of nicotinamide (120 mg/kg body weight [b.wt.]), then streptozotocin (200 mg/kg b.wt.) after 15 min. Hydroethanolic extracts (80%) of the plants and algae under investigation were prepared. The extracts were orally administered to nicotinamide-streptozotocin-induced diabetic mice by a gastric tube at doses 10 or 50 mg/kg b.wt. for 1 week. The antidiabetic activity was assessed by detection of serum glucose concentrations at the fasting state and after 2 h of oral glucose loading (4.2 mg/kg b.wt.). Extracts prepared from Cassia acutifolia, Fraxinus ornus, Salix aegyptiaca, Cichorium intybus, and Eucalyptus globulus showed the highest antihyperglycemic activity among the tested plants. Extracts prepared from Sonchus oleraceus, Bougainvillea spectabilis (leaves), Plantago psyllium (seeds), Morus nigra (leaves), and Serena repens (fruits) were found to have antihyperglycemic potentials. Extracts prepared from Caulerpa lentillifera and Spirulina versicolor showed the most potent antihyperglycemic activity among the tested algae. However, some of the tested plants have insulinotropic effects, all assessed algae have not. Identification of lead compounds from these plants and algae for novel antidiabetic drug development is recommended.

  3. Effects of exotic Eucalyptus spp. plantations on soil properties in and around sacred natural sites in the northern Ethiopian Highlands

    Directory of Open Access Journals (Sweden)

    Alemayehu Wassie

    2016-04-01

    Full Text Available Species of the genus Eucalyptus (common name eucalyptus are widely planted all across Ethiopia—including on large areas of land previously allocated to food production. In recent decades eucalyptus has also increasingly been planted on lands around and within “church forests,” sacred groves of old-aged Afromontane trees surrounding Ethiopian Orthodox Tewahido churches. These revered holy sites have long been recognized for their cultural values and also for their ecosystem services—including their potential to support species conservation and restoration, as church forests are some of the only remaining sanctuaries for many of Ethiopia’s indigenous and endemic plant and animal populations. Ethiopian Orthodox church communities have a long history of planting and nurturing indigenous tree seedlings to sustain church forest groves. However, due to the fast-growing nature of eucalyptuscombined with its widely recognized socio-economic benefits (as fuelwood, charcoal, construction wood, etc., this introduced species has been widely plantedaround church forests—in some cases even replacing native tree species within church forests themselves. In many developing country contexts the introduction of exotic eucalyptus has been shown to have ecological impacts ranging from soil nutrient depletion, to lowering water tables, to allelopathic effects. In this study, we collected soil samples from indigenous forest fragments (church forests, adjacent eucalyptus plantations, and surrounding agricultural land to examine how eucalyptus plantations in Ethiopian Orthodox church communitiesmight impact soil quality relative to alternative land uses. Soil properties, including organic matter, pH, total nitrogen, and total phosphorus were measured in samples across 20 church forest sites in South Gondar, East Gojjam, West Gojjam, Awi, and Bahir Dar Liyu zones in the Amhara Region of the northern Ethiopian Highlands. Findings indicate that although soil in

  4. Three-year study of fast-growing trees in degraded soils amended with composts: Effects on soil fertility and productivity.

    Science.gov (United States)

    Madejón, Paula; Alaejos, Joaquin; García-Álbala, José; Fernández, Manuel; Madejón, Engracia

    2016-03-15

    Currently, worries about the effects of intensive plantations on long-term nutrient supply and a loss of productivity have risen. In this study two composts were added to degraded soils where this type of intensive crops were growing, to avoid the soil fertility decrease and try to increase biomass production. For the experiment, two degraded soils in terms of low organic carbon content and low pH were selected in South-West Spain: La Rábida (RA) and Villablanca (VI) sites. Both study sites were divided into 24 plots. In RA, half of the plots were planted with Populus x canadensis "I-214"; the other half was planted with Eucalyptus globulus. At the VI site, half of the plots were planted with Paulownia fortunei, and the other plots were planted with Eucalyptus globulus. For each tree and site, three treatments were established (two organic composts and a control without compost), with four replications per treatment. The organic amendments were "alperujo" compost, AC, a solid by-product from the extraction of olive oil, and BC, biosolid compost. During the three years of experimentation, samples of soils and plants were analyzed for studying chemical and biochemical properties of soil, plant growth and plant nutritional status and biomass production. The composts increased total organic carbon, water-soluble carbon, nutrients and pH of soil only in the most acidic soil. Soil biochemical quality was calculated with the geometric mean of the enzymatic activities (Dehydrogenase, β-glucosidase, Phosphatase and Urease activities) determined in soils. The results showed a beneficial improvement in comparison with soils without compost. However, the best results were found in the growth and biomass production of the studied trees, especially in Eucalyptus. Nutritional levels of leaves of the trees were, in general, in the normal established range for each species, although no clear effect of the composts was observed. The results of this study justify the addition of

  5. Process design and economics of a flexible ethanol-butanol plant annexed to a eucalyptus kraft pulp mill.

    Science.gov (United States)

    Pereira, Guilherme C Q; Braz, Danilo S; Hamaguchi, Marcelo; Ezeji, Thaddeus C; Maciel Filho, Rubens; Mariano, Adriano P

    2018-02-01

    This work proposes a strategy, from a process design standpoint, for pulp companies to enter the Brazilian ethanol market. The flexible plant converts eucalyptus-derived glucose to either ethanol or butanol (according to market conditions) and xylose only to butanol production. Depending on the biomass pretreatment technology, Monte Carlo simulations showed that the Net Present Value (NPV) of the flexible plant increases by 20-28% in relation to an ethanol-dedicated plant. Whereas the lower costs of the steam explosion technology turns the investment more attractive (NPV = 184 MMUSD; IRR = 29%), the organosolv technology provides better flexibility to the plant. This work also shows that excessive power consumption is a hurdle in the development of flash fermentation technology chosen for the flexible plant. These results indicate that conventional batch fermentation is preferable if the enzymatic hydrolysis step operates with solids loading up to 20 wt%. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Variation in composition and yield of foliage oil of eucalyptus polybractea

    International Nuclear Information System (INIS)

    Iqbal, Z.; Akhtar, M.; Qureshi, T.M.; Ahhter, J.; Ahmad, R.

    2011-01-01

    Eucalyptus polybractea (blue mallee) is the essential oil rich species used in the commercial production of pharmaceutical-grade Eucalyptus oil in Australia. This species was grown at Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan during 2004-08 to investigate the quantity and quality of its foliage oil. The oils were extracted by hydro-distillation method, from the leaves of four year aged ten E. polybractea plants. The data showed a significant intra-species variation in their oil contents (29.3 to 41.8 mg g-1 fresh weight of leaves). Out of ten plants eight contained oil >30 mg g/sup -1/ fresh weight of leaves. The components of the extracted oils varied from 12-26 as detected by GC/FID on Carbowax 20 M packed glass column. Among all the oil components, 1, 8-cineole was the major compound (91.7-94.2 %), while the other identified compounds were alpha-pinene (0-1.2 %), beta-pinene (0.4-2.3 %), limonene (0.2-1.3 %), p-cymene (1.23-2.75 %), and terpinene-4-ol (0.6-0.92 %). The extracted oils from all the Eucalyptus polybractea plants contained high amount of 1, 8-cineole (>90 %), therefore, classified as species of high quality medicinal oil. (author)

  7. Using chlorophyll fluorescence to determine stress in Eucalyptus ...

    African Journals Online (AJOL)

    Using chlorophyll fluorescence to determine stress in Eucalyptus grandis seedlings: scientific paper. ... Southern Forests: a Journal of Forest Science ... factors affect the functioning of the photosynthetic system, the status of the photosynthetic apparatus is a good indicator of the plant in terms of stress and stress adaptation.

  8. BRF

    African Journals Online (AJOL)

    SARAH

    30 sept. 2014 ... They can help improve soil chemical properties and are therefore useful in the sustainable management of fertility of ..... de Eucalyptus globulus avait une influence significative ... Production, Development in Soil Science 3,.

  9. VEGETATION OF CHENCHA HIGHLANDS IN SOUTHERN ETHIOPIA

    African Journals Online (AJOL)

    ADMIN

    inter alia, by environmental factors including soil properties, slope and aspect which varied significantly .... species percentage cover in each community, families and growth form and life form of each species are ..... Eucalyptus globulus Labill.

  10. Phytophagy on eucalyptus plants increases the development and reproduction of the predator Podisus nigrispinus (Hemiptera: Pentatomidae = Fitofagia em plantas de eucaliptos aumenta o desenvolvimento e a reprodução do predador Podisus nigrispinus (Hemiptera: Pentatomidae.

    Directory of Open Access Journals (Sweden)

    Anderson Mathias Holtz

    2011-04-01

    Full Text Available The effect of plant feeding on biological aspects of Podisus nigrispinus (Dallas (Heteroptera: Pentatomidae was evaluated. Nymphs and adults of this predator were fed with Tenebrio molitor L. (Coleoptera: Tenebrionidae pupae on Eucalyptus urophylla plants inthe field or only with pupae of this prey in the laboratory. The development and nymphal survival, as well as the pre-oviposition period, number of egg masses, number, fertility and viability of eggs and the longevity of females of this predator were evaluated. The eucalyptus plants improved the development of P. nigrispinus. This demonstrates that this predator can present higher population growth with eucalyptus plants and T. molitor pupae than in the laboratory (controlled conditions only with this prey. These plants can supply nutrients that can the population growth and efficiency of P. nigrispinus for biological control in eucalyptus plantations.O efeito da alimentação em plantas sobre os aspectos biológicos de Podisus nigrispinus (Hemiptera: Pentatomidae foi avaliado. Ninfas e adultos desse predador foram alimentados com pupas de Tenebrio molitor L. (Coleotpera: Tenebrionidae em plantas de Eucalyptus urophylla no campo ou, apenas, em laboratório. O desenvolvimento e a sobrevivência ninfal desse predador, além do período de pré-oviposição, número de posturas, viabilidade dos ovos e afertilidade e longevidade de fêmeas foram avaliados. A planta de eucalipto proporcionou um incremento no desenvolvimento de P. nigrispinus. Isto demonstra que esse predador pode apresentar maior crescimento populacional com plantas de eucalipto e pupas de T. molitor alimentado com apenas a presa (condições controladas. A planta pode fornecer nutrientes que aumentam o crescimento populacional e a eficiência de P. nigrispinus para o controle biológico em plantios de eucalipto.

  11. Liquid nitrogen pretreatment of eucalyptus sawdust and rice hull for enhanced enzymatic saccharification.

    Science.gov (United States)

    Castoldi, Rafael; Correa, Vanesa G; de Morais, Gutierrez Rodrigues; de Souza, Cristina G M; Bracht, Adelar; Peralta, Rosely A; Peralta-Muniz Moreira, Regina F; Peralta, Rosane M

    2017-01-01

    In this work, liquid nitrogen was used for the first time in the pretreatment of plant biomasses for purposes of enzymatic saccharification. After treatment (cryocrushing), the initial rates of the enzymatic hydrolysis of eucalyptus sawdust and rice hull were increased more than ten-fold. Cryocrushing did not modify significantly the contents of cellulose, hemicellulose and lignin in both eucalyptus sawdust and rice hulls. However, substantial disorganization of the lignocellulosic materials in consequence of the pretreatment could be observed by electron microscopy. Cryocrushing was highly efficient in improving the saccharification of the holocellulose component of the plant biomasses (from 4.3% to 54.1% for eucalyptus sawdust and from 3.9% to 40.6% for rice hull). It is important to emphasize that it consists in a simple operation with low requirements of water and chemicals, no corrosion, no release of products such as soluble phenolics, furfural and hydroxymethylfurfural and no waste generation. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Heterosis may result in selection favouring the products of long-distance pollen dispersal in Eucalyptus.

    Directory of Open Access Journals (Sweden)

    João Costa E Silva

    Full Text Available Using native trees from near the northern and southern extremities of the relatively continuous eastern distribution of Eucalyptus globulus in Tasmania, we compared the progenies derived from natural open-pollination (OP with those generated from within-region and long-distance outcrossing. Controlled outcrossing amongst eight parents - with four parents from each of the northern and southern regions - was undertaken using a diallel mating scheme. The progeny were planted in two field trials located within the species native range in southern Tasmania, and their survival and diameter growth were monitored over a 13-year-period. The survival and growth performances of all controlled cross types exceeded those of the OP progenies, consistent with inbreeding depression due to a combination of selfing and bi-parental inbreeding. The poorer survival of the northern regional (♀N♂N outcrosses compared with the local southern regional outcrosses (♀S♂S indicated differential selection against the former. Despite this mal-adaptation of the non-local ♀N♂N crosses at both southern sites, the survival of the inter-regional hybrids (♀N♂S and ♀S♂N was never significantly different from that of the local ♀S♂S crosses. Significant site-dependent heterosis was detected for the growth of the surviving long-distance hybrids. This was expressed as mid-parent heterosis, particularly at the more northern planting site. Heterosis increased with age, while the difference between the regional ♀N♂N and ♀S♂S crosses remained insignificant at any age at either site. Nevertheless, the results for growth suggest that the fitness of individuals derived from long-distance crossing may be better at the more northern of the planting sites. Our results demonstrate the potential for early-age assessments of pollen dispersal to underestimate realised gene flow, with local inbreeding under natural open-pollination resulting in selection favouring the

  13. Metabolic responses of Eucalyptus species to different temperature regimes

    NARCIS (Netherlands)

    Mokochinski, Joao Benhur; Mazzafera, Paulo; Sawaya, Alexandra Christine Helena Frankland; Mumm, Roland; Vos, de Ric Cornelis Hendricus; Hall, Robert David

    2018-01-01

    Species and hybrids of Eucalyptus are the world's most widely planted hardwood trees. They are cultivated across a wide range of latitudes and therefore environmental conditions. In this context, comprehensive metabolomics approaches have been used to assess how different temperature regimes may

  14. Propriedades de chapas tipo OSB, fabricadas com partículas acetiladas de madeiras de Eucalyptus grandis, Eucalyptus urophylla, Eucalyptus cloeziana e Pinus elliottii Properties of OSB manufactured with wood strands of Eucalyptus grandis, Eucalyptus urophylla, Eucalyptus cloeziana and Pinus elliottii

    Directory of Open Access Journals (Sweden)

    Carla Priscilla Távora Cabral

    2006-08-01

    Full Text Available Este trabalho teve como objetivo avaliar as propriedades de chapas de 0riented Strand Board (OSB, fabricadas com flocos de madeira de Eucalyptus grandis, Eucalyptus urophylla e Eucalyptus cloeziana e oriundas dos Municípios de Ponte Alta e Três Marias, no Estado de Minas Gerais. As massas específicas básicas das três espécies de eucaliptos das duas regiões foram, respectivamente: Ponte Alta (0,55; 0,61; e 0,70 g/cm³ e Três Márias (0,56; 0,58; e 0,69 g/cm³. Quando necessário, para manter as massas específicas das chapas próximas de 0,70 g/cm³ foram acrescentadas às partículas de madeira de eucalipto partículas de madeira de Pinus elliottii, oriundo da cidade de Viçosa, com massa específica de 0,45 g/cm³. Os flocos foram gerados nas dimensões médias de 90,00 x 20,00 x 0,46 mm. O adesivo utilizado foi o fenol-formaldeído, na proporção de 8% de sólidos, em relação à massa seca de partículas. Parte dos flocos de eucaliptos foram acetilados. As chapas foram prensadas à temperatura de 170 °C e 32 kgf/cm² de pressão. As propriedades das chapas foram determinadas segundo as normas da ABNT NBR 14810-3 (2002 e ASTM-D 1037 (1991. Os resultados foram comparados utilizando-se as normas ANSI/A - 208.1 (1993 e CSA 0437-93 (1993. As chapas contendo partículas acetiladas foram mais estáveis e adsorveram menos umidade. Na tração perpendicular, observou-se que as chapas contendo 100% de flocos acetilados apresentaram resultados inferiores ao estipulado pela norma CSA O437-0/93 (1993. A resistência ao arrancamento de parafuso, módulo de ruptura (paralelo e perpendicular e compressão longitudinal (perpendicular, foi reduzida pela acetilação nas chapas contendo 100% dos flocos acetilados. As espécies que apresentaram, numericamente, as maiores médias para resistência mecânica foram: Eucalyptus grandis não acetilado (dureza Janka e Eucalyptus cloeziana misturado com Pinus sp (módulo de ruptura. Somente a resistência

  15. Growth and nutrition of eucalyptus clones seedlings inoculated with mycorrhizal fungi

    Directory of Open Access Journals (Sweden)

    Francisco de Sousa Lima

    2014-06-01

    Full Text Available Eucalyptus is one of the most planted forest species, in Brazil, due to its rapid growth and high economic yield. Arbuscular mycorrhizal fungi improve the seedlings nutritional and phytosanitary status, besides increasing their resistance to biotic and abiotic stress. This study aimed to evaluate the effect of inoculation with arbuscular mycorrhizal fungi species on the growth and nutrition of different eucalyptus clones seedlings. The experiment was conducted under greenhouse conditions, in a randomized blocks design and a 5x5 factorial scheme (five fungal species and five eucalyptus clones, with five replications. In general, the mycorrhizal symbiosis significantly increased the growth and nutrition of eucalyptus seedlings, when compared to the non-inoculated seedlings. The most efficient interaction occured between the 2361 clone and the Entrophospora infrequens fungus, with increases of 107.3% and 120.6%, for the shoot and root dry biomass yield, and 107.7%, 94.1% and 103.3%, respectively for the accumulation of N, P and K in the seedlings shoots. All the fungal species studied showed a high absolute compatibility index with eucalyptus clones. The Glomus manihots and E. infrequens fungi presented a higher functional compatibility index with the clones tested. The 5204 clone showed 75% of compatibility with the fungi evaluated.

  16. Did Eucalyptus contribute to environment degradation? Implications from a dispute on causes of severe drought in Yunnan and Guizhou, China

    Directory of Open Access Journals (Sweden)

    WenJun Zhang

    2012-06-01

    Full Text Available Various viewpoints were proposed to explain the causes of recent years' severe drought occurred in Yunnan and Guizhou, China. In general there are two parties of viewpoints, the Eucalyptus cause and climate change cause. I think Yunnan-Guizhou drought has been mainly caused by abnormal climate change. Eucalyptus was not significant in the formation of Yunnan-Guizhou drought. However, the forestation effect of Eucalyptus in China was not good. Environment quality and biodiversity in Eucalyptus plantation forests has been degrading in last decades. Enhancement of alleopathy of Eucalyptus trees under drought conditions would partly contribute to biodiversity reduction and environment degradation in Eucalyptus plantation forests. For existing Eucalyptus plantation forests of Yunnan, I suggest that some improvement measures should be adopted. Artificial weeding and cleaning in Eucalyptus plantation forests should be banned. Density of Eucalyptus trees needs to be reduced. Biodiversity should be artificially improved in Eucalyptus plantation forests. In the future, the mountains and lands with better vegetation cover must not be reclaimed for Eucalyptus planting. Eucalyptus plantation forests should be made in barren mountains and lands with poor biodiversity.

  17. Diagnosing foliar nutrient dynamics of Eucalyptus grandis in ...

    African Journals Online (AJOL)

    Fertilisation is one of the most cost-effective methods of increasing and maintaining the productivity of Eucalyptus grandis plantations in South Africa. This silvicultural practice can be optimised by using the foliar nutrient ratios measured in plants at maximum growth as a guideline for fertiliser application. The foliar nutrient ...

  18. Optimization of the process of aromatic and medicinal plant maceration in grape marc distillates to obtain herbal liqueurs and spirits.

    Science.gov (United States)

    Rodríguez-Solana, Raquel; Vázquez-Araújo, Laura; Salgado, José Manuel; Domínguez, José Manuel; Cortés-Diéguez, Sandra

    2016-11-01

    Herbal liqueurs are alcoholic beverages produced by the maceration or distillation of aromatic and medicinal plants in alcohol, and are also highly valued for their medicinal properties. The process conditions, as well as the number and quantity of the plants employed, will have a great influence on the quality of the liqueur obtained. The aim of this research was to optimize these important variables. A Box-Benhken experimental design was used to evaluate the independent variables: alcohol content, amount of plant and time during the experimental maceration of plants in grape marc distillate. Four plants were assessed, with the main compound of each plant representing the dependent variable evaluated with respect to following the evolution of the maceration process. Bisabolol oxide A in Matricaria recutita L., linalool in Coriander sativum L. and eucalyptol in Eucalyptus globulus Labill. were quantified using a gas chromatography-flame ionization detector. Glycyrrhizic acid in Glycyrrhiza glabra L was determined using a high-performance liquid chromatography-diode array detector. Other dependent variables were also evaluated: total phenolic content, color parameters and consumer preference (i.e. appearance). The experimental designs allowed the selection of the optimal maceration conditions for each parameter, including the preference score of consumers: 70% (v/v) of ethanol, 40 g L -1 plant concentration and a maceration process of 3 weeks. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  19. Potential implications for expansion of freeze-tolerant eucalyptus plantations on water resources in the southern United States

    Science.gov (United States)

    James M. Vose; Chelcy F. Miniat; Ge Sun; Peter V. Caldwell

    2014-01-01

    The potential expansion of freeze-tolerant (FT) Eucalyptus plantations in the United States has raised concerns about the implications for water resources. Modeling was used to examine the potential effects of expanding the distribution of FT Eucalyptus plantations in US Department of Agriculture Plant Hardiness Zones 8b and...

  20. Stimulatory effect of eucalyptus essential oil on the germination and early growth of Eucalyptus grandis seedlings Efeito estimulante do óleo essencial de eucalipto na germinação e crescimento inicial de mudas de Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Ricardo Bemfica Steffen

    2010-12-01

    Full Text Available

    The use of essential oil and extracts from plants is becoming an efficient alternative in the biostimulation on growth and protection of plants. The aim of this work was to evaluate the use of leaf essential oil of Eucalyptus grandis on the germination and the  development of Eucalyptus grandis seedlings in nursery conditions. The eucalyptus seeds were exposed to the concentrations of 0, 10, 25, 50, 75 and 100 μL L-1 of the essential oil in controlled conditions. The eucalyptus seedlings were sprayed with 0, 20, 30, 40, 50 and 60 μL L-1 of the essential oil per plant, at intervals of seven days. The effect of this application on the seedling development were analyzed after 30 and 60 days. The results show that the germination was significantly higher when the seeds were exposed to 25 μL L-1 of the essential oil. The application of essential oil in the concentration of 30 and 40 μL L-1 provided higher growth of the aerial part and of the roots in greenhouse conditions, being an effective alternative to biostimulation the vegetative growth of eucalyptus seedlings.

    doi: 10.4336/2010.pfb.30.63.199

    A utilização de extratos e óleos essenciais de plantas vem sendo uma alternativa eficiente no bioestímulo do crescimento e proteção vegetal. O trabalho teve por objetivo avaliar a utilização do óleo essencial extraído de folhas de Eucalyptus grandis sobre a germinação e o desenvolvimento inicial de mudas de eucalipto em condições controladas, em casa de vegetação. Sementes de eucalipto foram incubadas em  concentrações de 0, 10, 25, 50, 75 e 100 μL L-1 do óleo essencial em ambiente controlado.  Posteriormente, pulverizou-se plântulas de eucalipto com 0, 20, 30, 40, 50 e 60 μL L-1 do óleo essencial por planta, em intervalos de sete dias. Avaliou-se o efeito do óleo sobre o desenvolvimento vegetal aos 30 e 60 dias após o transplante. Os resultados evidenciam que a germinação foi significativamente maior

  1. Transcriptome and proteome analysis of Eucalyptus infected with Calonectria pseudoreteaudii.

    Science.gov (United States)

    Chen, Quanzhu; Guo, Wenshuo; Feng, Lizhen; Ye, Xiaozhen; Xie, Wanfeng; Huang, Xiuping; Liu, Jinyan

    2015-02-06

    Cylindrocladium leaf blight is one of the most severe diseases in Eucalyptus plantations and nurseries. There are Eucalyptus cultivars with resistance to the disease. However, little is known about the defense mechanism of resistant cultivars. Here, we investigated the transcriptome and proteome of Eucalyptus leaves (E. urophylla×E. tereticornis M1), infected or not with Calonectria pseudoreteaudii. A total of 8585 differentially expressed genes (|log2 ratio| ≥1, FDR ≤0.001) at 12 and 24hours post-inoculation were detected using RNA-seq. Transcriptional changes for five genes were further confirmed by qRT-PCR. A total of 3680 proteins at the two time points were identified using iTRAQ technique.The combined transcriptome and proteome analysis revealed that the shikimate/phenylpropanoid pathway, terpenoid biosynthesis, signalling pathway (jasmonic acid and sugar) were activated. The data also showed that some proteins (WRKY33 and PR proteins) which have been reported to involve in plant defense response were up-regulated. However, photosynthesis, nucleic acid metabolism and protein metabolism were impaired by the infection of C. pseudoreteaudii. This work will facilitate the identification of defense related genes and provide insights into Eucalyptus defense responses to Cylindrocladium leaf blight. In this study, a total of 130 proteins and genes involved in the shikimate/phenylpropanoid pathway, terpenoid biosynthesis, signalling pathway, cell transport, carbohydrate and energy metabolism, nucleic acid metabolism and protein metabolism in Eucalyptus leaves after infected with C. pseudoreteaudii were identified. This is the first report of a comprehensive transcriptomic and proteomic analysis of Eucalyptus in response to Calonectria sp. Copyright © 2014 Elsevier B.V. All rights reserved.

  2. A Newly Identified Passive Hyperaccumulator Eucalyptus grandis × E. urophylla under Manganese Stress.

    Science.gov (United States)

    Xie, Qingqing; Li, Zhenji; Yang, Limin; Lv, Jing; Jobe, Timothy O; Wang, Qiuquan

    2015-01-01

    Manganese (Mn) is an essential micronutrient needed for plant growth and development, but can be toxic to plants in excess amounts. However, some plant species have detoxification mechanisms that allow them to accumulate Mn to levels that are normally toxic, a phenomenon known as hyperaccumulation. These species are excellent candidates for developing a cost-effective remediation strategy for Mn-polluted soils. In this study, we identified a new passive Mn-hyperaccumulator Eucalyptus grandis × E. urophylla during a field survey in southern China in July 2010. This hybrid can accumulate as much as 13,549 mg/kg DW Mn in its leaves. Our results from Scanning Electron Microscope (SEM) X-ray microanalysis indicate that Mn is distributed in the entire leaf and stem cross-section, especially in photosynthetic palisade, spongy mesophyll tissue, and stem xylem vessels. Results from size-exclusion chromatography coupled with ICP-MS (Inductively coupled plasma mass spectrometry) lead us to speculate that Mn associates with relatively high molecular weight proteins and low molecular weight organic acids, including tartaric acid, to avoid Mn toxicity. Our results provide experimental evidence that both proteins and organic acids play important roles in Mn detoxification in Eucalyptus grandis × E. urophylla. The key characteristics of Eucalyptus grandis × E. urophylla are an increased Mn translocation facilitated by transpiration through the xylem to the leaves and further distribution throughout the leaf tissues. Moreover, the Mn-speciation profile obtained for the first time in different cellular organelles of Eucalyptus grandis × E. urophylla suggested that different organelles have differential accumulating abilities and unique mechanisms for Mn-detoxification.

  3. Growth response at age 10 years of five Eucalyptus genotypes ...

    African Journals Online (AJOL)

    Thus, following the 1999 drought event a trial was established in a drought-prone area, planted to a number of genotypes that were deemed to be more tolerant to water stress than Eucalyptus grandis. Each species was planted at stand densities of 816, 1 600 and 2 066 stems ha-1, where 1 600 stems ha-1 represented the ...

  4. BALANCE OF WATER AND ENERGY FOR EUCALYPTUS PLANTATIONS WITH PARTIAL SOIL COVER

    Directory of Open Access Journals (Sweden)

    Mariana Gonçalves dos Reis

    2014-03-01

    Full Text Available http://dx.doi.org/10.5902/1980509813329Eucalyptus plots with initial development ages presented discontinuity in soil cover, resulting in greaterexposure of the leaves to wind and solar radiation, which alters soil-plant-atmosphere interactions. Theobjective of this study was to study the components of the water and energy balances along the first yearof eucalyptus development in the Brazilian coastal plain region. The experimental site is located in anarea belonging to the company Fibria in the municipality of Aracruz, Espírito Santo state, Brazil. Thespace between the planted eucalyptus trees in the area studied was 3 x 3 m and the data of planting wason August 15th , 2004. The period of study lasted from the planting date until the plot reached an ageof 19 months. It was verified that there was a greater availability of energy during the summer and theprecipitation directly influenced the energy balance where during the period of study the energy available necessary for evapotranspiration was always greater than the fraction necessary for heating the soil-plantatmospheresystem, presenting a λE/Rn ratio of 59.57%. It was also observed that the water balance with themodeled evapotranspiration showed a good correspondence with the observed moisture content, presentinga determination coefficient of 0,94. In the majority of trees, greater indices of leaf and root system areasfavored evapotranspiration, indicating that most energy available was utilized for changing the phase ofwater

  5. A COMPARATIVE STUDY ON MACROSSOPIC, MICROSCOPIC AND PHYTOCHEMICAL PROPERTIES OF EUCALYPTUS SPEJES, SPEJES, CULTIVATED IN IRAN, WITH STANDARD SPECIES

    Directory of Open Access Journals (Sweden)

    samsam shariat sadeghi-aliabadi

    1993-07-01

    Full Text Available The aim of this study is to compare the volatile oil of staendard species of Eucalyptus with those species cultivated in Iran. The investigation was carried out as bellow:"nA The seeds of elven species were supplied from Germany and cultivated in Research Center of Agriculture of Isfahan and then extracted for volatile oil and cineole."nB Besides, we supplied 2 species from Isfahan, 7 species from Fars province, 9 from Khuzestan province, one from Tehran and one from Hormozgan, province."nC We extracted total volatile oil of 28 E. species, then injected to G. G. column and the results were as follow:"n1 - In the following species : E. saligna, E. loxophleba and E. Stratycalyx from Esfahan province. E."nloxophleba, E.camaldulensis, E.stratycalyx, intertexta and E. caleicultrix from Fars"nprovince. E. Stratycalyx, E. OCCidsntalis, E. gillii and E. sargentii from khozestan prcvince. E. globulus from Tehran and E. camaldulensis from Hormozgan province. The amount of essential oil extracted from the mentioned species was in accord with the standard of pharmacopial level. But the extracted oil from others was not as standard levl."n2 - E. gillii, E. camaldulensis, E. sargentii and OCCidentalis from khouzestan and the species of"nE.intertexta, E. camaldulensis, E. lansdownean from fars and the species of E. camaldulensis, E. saligna from Esfahan and the species of E. globulus from Tehran, the amount of measured cineol was in accordance the standard pharmacopeial level."n3 - Some of the species studied such as : were qualified for the amount of Alpha and Beta pinen.

  6. Dynamics of adventitious rooting in mini-cuttings of Eucalyptus benthamii x Eucalyptus dunnii=Dinamica de enraizamento adventício em miniestacas de Eucalyptus benthamii x Eucalyptus dunnii

    Directory of Open Access Journals (Sweden)

    Antônio Natal Gonçalves

    2012-04-01

    Full Text Available It is possible to determine the optimum time for permanence of vegetative propagules (mini-cuttings inside a greenhouse for rooting, and this value can be used to optimize the structure of the nursery. The aim of this study was to determine the dynamics of adventitious rooting in mini-cuttings of three clones of Eucalyptus benthamii x Eucalyptus dunnii. Sprouts of H12, H19 and H20 clones were collected from mini-stumps that were planted in gutters containing sand and grown in a semi-hydroponic system. The basal region of the mini-cuttings was immersed in 2,000 mg L-1 indole-3-butyric acid (IBA solution for 10 seconds. The rooting percentage of the mini-cuttings, the total length of the root system and the rooting rate per mini-cutting were also evaluated at 0 (time of planting, 7, 14, 21, 28, 35, 42, 49 and 56 days. We used logistic and exponential regression to mathematically model the speed of rhizogenesis. The rooting percentage was best represented as a logistic model, and the total length of the root system was best represented as an exponential model. The clones had different speeds of adventitious rooting. The optimum time for permanence of the mini-cuttings inside the greenhouse for rooting was between 35 and 42 days, and varied depending on the genetic material.O tempo ideal de permanência de propágulos vegetativos (miniestacas no interior da casa de vegetação para a rizogênese é possível de ser determinado matematicamente, o que pode otimizar as instalações do viveiro. O objetivo deste estudo foi determinar a dinâmica de enraizamento de miniestacas de três clones de Eucalyptus benthamii x Eucalyptus dunnii. Brotações dos clones H19, H12 e H20 foram coletadas de minicepas plantadas em canaletão com areia e cultivadas sob sistema semi-hidropônico. A região basal da miniestaca foi imersa em solução de 2.000 mg L-1 de ácido indolbutírico (AIB por 10 segundos. A porcentagem de enraizamento de miniestacas, o comprimento

  7. Equations for predicting biomass of six introduced tree species, island of Hawaii

    Science.gov (United States)

    Thomas H. Schukrt; Robert F. Strand; Thomas G. Cole; Katharine E. McDuffie

    1988-01-01

    Regression equations to predict total and stem-only above-ground dry biomass for six species (Acacia melanoxylon, Albizio falcataria, Eucalyptus globulus, E. grandis, E. robusta, and E. urophylla) were developed by felling and measuring 2- to 6-year-old...

  8. Effects of autohydrolysis of Eucalyptus urograndis and Eucalyptus grandis on influence of chemical components and crystallinity index.

    Science.gov (United States)

    da Silva Morais, Alaine Patrícia; Sansígolo, Cláudio Angeli; de Oliveira Neto, Mario

    2016-08-01

    Samples of Eucalyptus urograndis and Eucalyptus grandis sawdust were autohydrolyzed in aqueous conditions to reach temperatures in the range 110-190°C and reaction times of 0-150min in a minireactor. In each minireactor were used a liquor:wood ratio (10:1 L:kg dry wood), in order to assess the effects of the autohydrolysis severity and the crystalline properties of cellulose. The content of extractives, lignin, holocellulose, cellulose, hemicelluloses and crystallinity index obtained from the solid fraction after autohydrolysis of sawdust were determined. This study demonstrated that the hemicelluloses were extensively removed at 170 and 190°C, whereas cellulose was partly degraded to Eucalyptus urograndis and Eucalyptus grandis sawdust. The lignin content decreased, while the extractives content increased. It was defined that during autohydrolysis, had a slight decreased on crystalline structure of cellulose of Eucalyptus urogandis and Eucalyptus grandis. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Bilans d'éléments minéraux dans de petits bassins versants forestiers de Galice (NW Espagne)

    OpenAIRE

    Dambrine , Etienne ,; Vega , José; Taboada , Teresa; Rodriguez , Luis; Fernandez , Cristina; Macias , Felipe; Gras , José

    2000-01-01

    International audience; Budgets of mineral elements in small forested catchments in Galicia (NW Spain). In order to assess the sustainability of mineral supply to fast growing Eucalyptus globulus and Pinus pinaster stands in Galice (NW Spain), we calculated (input-output) nutrient budgets in three small catchments: the Castrove and Jabali catchments with eucalyptus on a base poor granite and the Arcos catchment with pine on granodiorite. In each catchment, the annual drainage output was lower...

  10. Antifungal Activity of Eucalyptus Oil against Rice Blast Fungi and the Possible Mechanism of Gene Expression Pattern.

    Science.gov (United States)

    Zhou, Li-Jun; Li, Fu-Rong; Huang, Li-Jie; Yang, Zhi-Rong; Yuan, Shu; Bai, Lin-Han

    2016-05-12

    Eucalyptus oil possesses a wide spectrum of biological activity, including anti-microbial, fungicidal, herbicidal, acaricidal and nematicidal properties. We studied anti-fungal activities of the leaf oil extracted from Eucalyptus. grandis × E. urophylla. Eleven plant pathogenic fungi were tested based on the mycelium growth rates with negative control. The results showed that Eucalyptus oil has broad-spectrum inhibitory effects toward these fungi. Remarkable morphological and structural alterations of hypha have been observed for Magnaporthe grisea after the treatment. The mRNA genome array of M. grisea was used to detect genes that were differentially expressed in the test strains treated by the Eucalyptus oil than the normal strains. The results showed 1919 genes were significantly affected, among which 1109 were down-regulated and 810 were up-regulated (p 2). According to gene ontology annotation analysis, these differentially expressed genes may cause abnormal structures and physiological function disorders, which may reduce the fungus growth. These results show the oil has potential for use in the biological control of plant disease as a green biopesticide.

  11. Genome-wide analysis of EgEVE_1, a transcriptionally active endogenous viral element associated to small RNAs in Eucalyptus genomes

    Directory of Open Access Journals (Sweden)

    Helena Sanches Marcon

    2017-02-01

    Full Text Available Abstract Endogenous viral elements (EVEs are the result of heritable horizontal gene transfer from viruses to hosts. In the last years, several EVE integration events were reported in plants by the exponential availability of sequenced genomes. Eucalyptus grandis is a forest tree species with a sequenced genome that is poorly studied in terms of evolution and mobile genetic elements composition. Here we report the characterization of E. grandis endogenous viral element 1 (EgEVE_1, a transcriptionally active EVE with a size of 5,664 bp. Phylogenetic analysis and genomic distribution demonstrated that EgEVE_1 is a newly described member of the Caulimoviridae family, distinct from the recently characterized plant Florendoviruses. Genomic distribution of EgEVE_1 and Florendovirus is also distinct. EgEVE_1 qPCR quantification in Eucalyptus urophylla suggests that this genome has more EgEVE_1 copies than E. grandis. EgEVE_1 transcriptional activity was demonstrated by RT-qPCR in five Eucalyptus species and one intrageneric hybrid. We also identified that Eucalyptus EVEs can generate small RNAs (sRNAs,that might be involved in de novo DNA methylation and virus resistance. Our data suggest that EVE families in Eucalyptus have distinct properties, and we provide the first comparative analysis of EVEs in Eucalyptus genomes.

  12. (+)-Aromadendrene as chiral starting material for the synthesis of sesquiterpenes

    NARCIS (Netherlands)

    Gijsen, H.J.M.

    1993-01-01

    One of the distillation tails of the oil of Eucalyptus globulus, which is commercially available, contains about 55-70% of (+)-aromadendrene ( 2 ), together with 10-15% of alloaromadendrene ( 3 ). In this thesis has been described

  13. Effects of Treatment Materials on the Physical Properties of Eucalyptus (Eucalyptus camaldulensis Dehn. Wood

    Directory of Open Access Journals (Sweden)

    Selahattin Bardak

    2011-04-01

    Full Text Available This study was designed to determine effects on retention and shrink levels of Eucalyptus (Eucalyptus camaldulensis Dehn. which treated with some commercial preservative types, borates, polyethylene glycol, and water repellents. In this study, four type impregnation chemicals were chosen: 1. Commercial preservative types, [Ammonium sulphate, Vacsol-WR WR and Immersol-WR (WR], 2. Borates chemicals, [Boric acid, Borax, Boric acid+ Borax] 3. Polyethylene glycol such as Polyethylene glycol (PEG-400 4. Water repellents [Styrene, Methylmetacrylate.]. As a result, retention % and shrink levels of Eucalyptus wood was lower treated with commercial preservative types and borates preservatives compare to other treatment chemicals. However, retention % levels of Eucalyptus wood treated with WR chemicals were highly significant levels. According to their leachability period although PEG 400 showed antishrink effectiveness other treatments didnt showed antishrink efficiency.

  14. Captura de carbono en rebrotes de eucalyptus globulus labill “eucaliptus” en motil, provincia de Otuzco del departamento de La Libertad-Perú

    OpenAIRE

    Alfaro Nureña, Rafael Otoniel

    2017-01-01

    It was determined biomass, carbon content in plots of 250 square meters in 10 stands of eucalyptus plantations in the village of Motil Agallpampa district of the province of Otuzco, La Libertad, Peru .Was determined models biomass and carbon was determined by regression analysis based on the method of least squares ,method presented by Adler (1980); for this combinations of independent variables dap and height were selected using a spreadsheet (Excel). The equations chosen were those with the...

  15. Carbon assimilation in Eucalyptus urophylla grown under high atmospheric CO2 concentrations: A proteomics perspective.

    Science.gov (United States)

    Santos, Bruna Marques Dos; Balbuena, Tiago Santana

    2017-01-06

    Photosynthetic organisms may be drastically affected by the future climate projections of a considerable increase in CO 2 concentrations. Growth under a high concentration of CO 2 could stimulate carbon assimilation-especially in C3-type plants. We used a proteomics approach to test the hypothesis of an increase in the abundance of the enzymes involved in carbon assimilation in Eucalyptus urophylla plants grown under conditions of high atmospheric CO 2 . Our strategy allowed the profiling of all Calvin-Benson cycle enzymes and associated protein species. Among the 816 isolated proteins, those involved in carbon fixation were found to be the most abundant ones. An increase in the abundance of six key enzymes out of the eleven core enzymes involved in carbon fixation was detected in plants grown at a high CO 2 concentration. Proteome changes were corroborated by the detection of a decrease in the stomatal aperture and in the vascular bundle area in Eucalyptus urophylla plantlets grown in an environment of high atmospheric CO 2 . Our proteomics approach indicates a positive metabolic response regarding carbon fixation in a CO 2 -enriched atmosphere. The slight but significant increase in the abundance of the Calvin enzymes suggests that stomatal closure did not prevent an increase in the carbon assimilation rates. The sample enrichment strategy and data analysis used here enabled the identification of all enzymes and most protein isoforms involved in the Calvin-Benson-Bessham cycle in Eucalyptus urophylla. Upon growth in CO 2 -enriched chambers, Eucalyptus urophylla plantlets responded by reducing the vascular bundle area and stomatal aperture size and by increasing the abundance of six of the eleven core enzymes involved in carbon fixation. Our proteome approach provides an estimate on how a commercially important C3-type plant would respond to an increase in CO 2 concentrations. Additionally, confirmation at the protein level of the predicted genes involved in

  16. A Newly Identified Passive Hyperaccumulator Eucalyptus grandis × E. urophylla under Manganese Stress.

    Directory of Open Access Journals (Sweden)

    Qingqing Xie

    Full Text Available Manganese (Mn is an essential micronutrient needed for plant growth and development, but can be toxic to plants in excess amounts. However, some plant species have detoxification mechanisms that allow them to accumulate Mn to levels that are normally toxic, a phenomenon known as hyperaccumulation. These species are excellent candidates for developing a cost-effective remediation strategy for Mn-polluted soils. In this study, we identified a new passive Mn-hyperaccumulator Eucalyptus grandis × E. urophylla during a field survey in southern China in July 2010. This hybrid can accumulate as much as 13,549 mg/kg DW Mn in its leaves. Our results from Scanning Electron Microscope (SEM X-ray microanalysis indicate that Mn is distributed in the entire leaf and stem cross-section, especially in photosynthetic palisade, spongy mesophyll tissue, and stem xylem vessels. Results from size-exclusion chromatography coupled with ICP-MS (Inductively coupled plasma mass spectrometry lead us to speculate that Mn associates with relatively high molecular weight proteins and low molecular weight organic acids, including tartaric acid, to avoid Mn toxicity. Our results provide experimental evidence that both proteins and organic acids play important roles in Mn detoxification in Eucalyptus grandis × E. urophylla. The key characteristics of Eucalyptus grandis × E. urophylla are an increased Mn translocation facilitated by transpiration through the xylem to the leaves and further distribution throughout the leaf tissues. Moreover, the Mn-speciation profile obtained for the first time in different cellular organelles of Eucalyptus grandis × E. urophylla suggested that different organelles have differential accumulating abilities and unique mechanisms for Mn-detoxification.

  17. Intercropped silviculture systems, a key to achieving soil fungal community management in eucalyptus plantations.

    Directory of Open Access Journals (Sweden)

    Caio T C C Rachid

    Full Text Available Fungi are ubiquitous and important contributors to soil nutrient cycling, playing a vital role in C, N and P turnover, with many fungi having direct beneficial relationships with plants. However, the factors that modulate the soil fungal community are poorly understood. We studied the degree to which the composition of tree species affected the soil fungal community structure and diversity by pyrosequencing the 28S rRNA gene in soil DNA. We were also interested in whether intercropping (mixed plantation of two plant species could be used to select fungal species. More than 50,000 high quality sequences were analyzed from three treatments: monoculture of Eucalyptus; monoculture of Acacia mangium; and a mixed plantation with both species sampled 2 and 3 years after planting. We found that the plant type had a major effect on the soil fungal community structure, with 75% of the sequences from the Eucalyptus soil belonging to Basidiomycota and 19% to Ascomycota, and the Acacia soil having a sequence distribution of 28% and 62%, respectively. The intercropping of Acacia mangium in a Eucalyptus plantation significantly increased the number of fungal genera and the diversity indices and introduced or increased the frequency of several genera that were not found in the monoculture cultivation samples. Our results suggest that management of soil fungi is possible by manipulating the composition of the plant community, and intercropped systems can be a means to achieve that.

  18. Intercropped Silviculture Systems, a Key to Achieving Soil Fungal Community Management in Eucalyptus Plantations

    Science.gov (United States)

    Rachid, Caio T. C. C.; Balieiro, Fabiano C.; Fonseca, Eduardo S.; Peixoto, Raquel Silva; Chaer, Guilherme M.; Tiedje, James M.; Rosado, Alexandre S.

    2015-01-01

    Fungi are ubiquitous and important contributors to soil nutrient cycling, playing a vital role in C, N and P turnover, with many fungi having direct beneficial relationships with plants. However, the factors that modulate the soil fungal community are poorly understood. We studied the degree to which the composition of tree species affected the soil fungal community structure and diversity by pyrosequencing the 28S rRNA gene in soil DNA. We were also interested in whether intercropping (mixed plantation of two plant species) could be used to select fungal species. More than 50,000 high quality sequences were analyzed from three treatments: monoculture of Eucalyptus; monoculture of Acacia mangium; and a mixed plantation with both species sampled 2 and 3 years after planting. We found that the plant type had a major effect on the soil fungal community structure, with 75% of the sequences from the Eucalyptus soil belonging to Basidiomycota and 19% to Ascomycota, and the Acacia soil having a sequence distribution of 28% and 62%, respectively. The intercropping of Acacia mangium in a Eucalyptus plantation significantly increased the number of fungal genera and the diversity indices and introduced or increased the frequency of several genera that were not found in the monoculture cultivation samples. Our results suggest that management of soil fungi is possible by manipulating the composition of the plant community, and intercropped systems can be a means to achieve that. PMID:25706388

  19. Intercropped silviculture systems, a key to achieving soil fungal community management in eucalyptus plantations.

    Science.gov (United States)

    Rachid, Caio T C C; Balieiro, Fabiano C; Fonseca, Eduardo S; Peixoto, Raquel Silva; Chaer, Guilherme M; Tiedje, James M; Rosado, Alexandre S

    2015-01-01

    Fungi are ubiquitous and important contributors to soil nutrient cycling, playing a vital role in C, N and P turnover, with many fungi having direct beneficial relationships with plants. However, the factors that modulate the soil fungal community are poorly understood. We studied the degree to which the composition of tree species affected the soil fungal community structure and diversity by pyrosequencing the 28S rRNA gene in soil DNA. We were also interested in whether intercropping (mixed plantation of two plant species) could be used to select fungal species. More than 50,000 high quality sequences were analyzed from three treatments: monoculture of Eucalyptus; monoculture of Acacia mangium; and a mixed plantation with both species sampled 2 and 3 years after planting. We found that the plant type had a major effect on the soil fungal community structure, with 75% of the sequences from the Eucalyptus soil belonging to Basidiomycota and 19% to Ascomycota, and the Acacia soil having a sequence distribution of 28% and 62%, respectively. The intercropping of Acacia mangium in a Eucalyptus plantation significantly increased the number of fungal genera and the diversity indices and introduced or increased the frequency of several genera that were not found in the monoculture cultivation samples. Our results suggest that management of soil fungi is possible by manipulating the composition of the plant community, and intercropped systems can be a means to achieve that.

  20. Ongoing molecular studies of Eucalyptus powdery mildew in Brazil

    Science.gov (United States)

    N. R. Fonseca; L. M. S. Guimaraes; R. P. Pires; Ned Klopfenstein; M. -S. Kim; A. C. Alfenas

    2016-01-01

    Powdery mildew diseases are caused by biotrophic fungi in the Erysiphales. These fungal pathogens are easily observed by the whitish powdery appearance caused by their colonization of the aerial surfaces on living plants (Stadnik & Rivera, 2001) (Figure 1). In Brazil, powdery mildew of Eucalyptus spp is increasing under the current nursery production...

  1. Uncovering the defence responses of Eucalyptus to pests and pathogens in the genomics age.

    Science.gov (United States)

    Naidoo, Sanushka; Külheim, Carsten; Zwart, Lizahn; Mangwanda, Ronishree; Oates, Caryn N; Visser, Erik A; Wilken, Febé E; Mamni, Thandekile B; Myburg, Alexander A

    2014-09-01

    Long-lived tree species are subject to attack by various pests and pathogens during their lifetime. This problem is exacerbated by climate change, which may increase the host range for pathogens and extend the period of infestation by pests. Plant defences may involve preformed barriers or induced resistance mechanisms based on recognition of the invader, complex signalling cascades, hormone signalling, activation of transcription factors and production of pathogenesis-related (PR) proteins with direct antimicrobial or anti-insect activity. Trees have evolved some unique defence mechanisms compared with well-studied model plants, which are mostly herbaceous annuals. The genome sequence of Eucalyptus grandis W. Hill ex Maiden has recently become available and provides a resource to extend our understanding of defence in large woody perennials. This review synthesizes existing knowledge of defence mechanisms in model plants and tree species and features mechanisms that may be important for defence in Eucalyptus, such as anatomical variants and the role of chemicals and proteins. Based on the E. grandis genome sequence, we have identified putative PR proteins based on sequence identity to the previously described plant PR proteins. Putative orthologues for PR-1, PR-2, PR-4, PR-5, PR-6, PR-7, PR-8, PR-9, PR-10, PR-12, PR-14, PR-15 and PR-17 have been identified and compared with their orthologues in Populus trichocarpa Torr. & A. Gray ex Hook and Arabidopsis thaliana (L.) Heynh. The survey of PR genes in Eucalyptus provides a first step in identifying defence gene targets that may be employed for protection of the species in future. Genomic resources available for Eucalyptus are discussed and approaches for improving resistance in these hardwood trees, earmarked as a bioenergy source in future, are considered. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  2. Carbon allocation to growth and storage in two evergreen species of contrasting successional status.

    Science.gov (United States)

    Piper, Frida I; Sepúlveda, Paulina; Bustos-Salazar, Angela; Zúñiga-Feest, Alejandra

    2017-05-01

    A prevailing hypothesis in forest succession is that shade-tolerant species grow more slowly than shade-intolerant species, across light conditions, because they prioritize carbon (C) allocation to storage. We examined this hypothesis in a confamilial pair of species, including one of the fastest-growing tree species in the world ( Eucalyptus globulus ) and a shade-tolerant, slow-growing species ( Luma apiculata ). Seedlings were subjected to one out of four combinations of light (high vs. low) and initial defoliation (90% defoliated vs. nondefoliated) for four months. Growth, C storage concentration in different organs, leaf shedding, and lateral shoot formation were measured at the end of the experiment. Eucalyptus globulus grew faster than L. apiculata in high light, but not in low light. Both species had lower C storage concentration in low than in high light, but similar C storage concentrations in each light condition. Defoliation had no effect on C storage, except in the case of the old leaves of both species, which showed lower C storage levels in response to defoliation. Across treatments, leaf shedding was 96% higher in E. globulus than in L. apiculata while, in contrast, lateral shoot formation was 87% higher in L. apiculata . In low light, E. globulus prioritized C storage instead of growth, whereas L. apiculata prioritized growth and lateral branching. Our results suggest that shade tolerance depends on efficient light capture rather than C conservation traits. © 2017 Botanical Society of America.

  3. Phytotoxic effects of aqueous leaf extracts of two eucalyptus SPP. against parthenium hysterophorus L

    International Nuclear Information System (INIS)

    Javaid, A.; Shah, M.B.M.

    2007-01-01

    The present study was carried out to investigate the phytotoxic effect of aqueous leaf extracts of two eucalyptus species viz. E citriodora Hook and E. camaldulensis Dehnh. Against the germination and seeding growth of alien aggressive weed parthenium hysterophorus L. The experiment was conducted in department of Mycology and plant Pathology in 2006. Aqueous leaf extracts of 2, 4, 6, 8 and 10% (w/v) of the two aforementioned Eucalyptus species were employed in the present study. Extracts of 2-8% concentration of both the Eucalyptus species significantly suppressed germination of the target weed species. A 10% extract of both the species completely arrested the germination. Aqueous extracts also reduced the root and shoot length of parthenium. Effect of extracts on seedling biomass was insignificant. (author)

  4. Primeiro registro de Fulgurodes sartinaria (Lepidoptera: Geometridae em plantas de Eucalyptus cloeziana (Myrtaceae (Nota Científica. First record of Fulgurodes sartinaria (Lepidoptera: geometridae in Eucalyptus cloeziana (Myrtaceae (Scientific Note.

    Directory of Open Access Journals (Sweden)

    Claubert Wagner Guimarães de MENEZES

    2013-03-01

    Full Text Available O objetivo deste trabalho foi registrar a oviposição e o desenvolvimento de uma nova espécie de lepidóptera associada à Eucalyptus cloeziana F. Muell, 1878 (Myrtaceae. Ovos, imaturos e adultos de Fulgurodes sartinaria Guenée, 1858 (Lepidoptera: Geometridae foram encontrados em plantas de E. cloeziana no município de Itamarandiba, Minas Gerais, Brasil. Este trabalho é o primeiro registro desse desfolhador em plantas de eucalipto. Ninfas de Brontocoris tabidus Signoret, 1852 (Heteroptera: Pentatomidae foram também observadas predando as lagartas de F. sartinaria, isto indica que este predador poderá ser um potencial agente de controle biológico da espécie. A ocorrência de F. sartinaria ovipositando e se desenvolvendo em plantas de E. cloeziana mostra que este lepidóptero pode se tornar um desfolhador importante da espécie, sendo recomendável sua inclusão em monitoramentos de pragas do eucalipto visando seu manejo integrado.The aim of this study was to record the oviposition and development of a new species of lepidopteran pests of Eucalyptus cloeziana F. Muell, 1878 (Myrtaceae. Eggs, immatures and adults of Fulgurodes sartinaria Guenée, 1858 (Lepidoptera: Geometridae were found in plants of E. cloeziana in Itamarandiba, Minas Gerais state, Brazil. This work is the first record of this defoliator in eucalyptus plants. Nymphs of the Brontocoris tabidus Signoret, 1852 (Heteroptera: Pentatomidae have also been observed preying on the larvae of F. sartinaria, this indicates that this predator is a probable potential biological control agent of the species. The occurrence of F. sartinaria developing and laying eggs on plants of E. cloeziana shows that this insect can become an important defoliator and it is recommended its inclusion in monitoring pest of eucalyptus for integrated pest management.

  5. Responses of Eucalyptus species to fertilizer applications made at ...

    African Journals Online (AJOL)

    Early research trials in South Africa have shown that Eucalyptus species generally respond positively to the addition of nutrients at planting. However, as most of these research trials were located in KwaZulu-Natal, it was important to investigate the nature of the response in other afforested regions of the country where this ...

  6. Thinning regimes and initial spacing for Eucalyptus plantations in Brazil.

    Science.gov (United States)

    Ferraz Filho, Antonio C; Mola-Yudego, Blas; González-Olabarria, José R; Scolforo, José Roberto S

    2018-01-01

    This study focuses on the effects of different thinning regimes on clonal Eucalyptus plantations growth. Four different trials, planted in 1999 and located in Bahia and Espírito Santo States, were used. Aside from thinning, initial planting density, and post thinning fertilization application were also evaluated. Before canopy closure, and therefore before excessive competition between trees took place, it was found that stands planted under low densities (667 trees per hectare) presented a lower mortality proportion when compared to stand planted under higher densities (1111 trees per hectare). However, diameter growth prior to thinning operations was not statistically different between these two densities, presenting an overall mean of 4.9 cm/year. After canopy closure and the application of the thinning treatments, it was found that thinning regimes beginning early in the life of the stand and leaving a low number of residual trees presented the highest diameter and height growth. Unthinned treatments and thinning regimes late in the life of the stand (after 5.5 years), leaving a large number of residual trees presented the highest values of basal area production. The choice of the best thinning regime for Eucalyptus clonal material will vary according to the plantation objective.

  7. Prospects of apicultural entrepreneurship in coastal districts of eastern India: a melissopalynological evaluation.

    Science.gov (United States)

    Upadhyay, Debasis; Bhattacharya, Swapan; Ferguson, David K; Bera, Subir

    2014-01-01

    A melissopalynological analysis of fifty-one natural honey samples (twenty four spring, fifteen summer and twelve winter) collected during 2010-2011 from two east-coastal districts (20(0)20/ to 22(0)11/ N, 82(0)39/ to 87(0)01/ E) of Orissa, India was performed. Out of 37 unifloral samples found 25 were contributed by Apis cerana indica, seven by A. dorsata and the remaining five by A. florea. Out of 14 multifloral samples five were contributed by A. cerana indica, five by A. dorsata and the remaining four by A. florea. Principal component analysis confirmed the palynological classification of the unifloral honey samples. Eighty-two bee-plant taxa belonging to forty four families were recovered. The predominant nectariferous taxa of the spring season were Acanthus ilicifolius, Avicennia marina, Bruguiera gymnorrhiza, Cocos nucifera, Eucalyptus globulus, Phoenix paludosa, Pongamia pinnata, Prosopis juliflora, Sonneratia apetala and Syzygium cumini. In the summer the predominant nectariferous taxa were Borassus flabellifer, C. nucifera, E. globulus, Syzygium cumini, Terminalia arjuna, Aegiceras corniculatum, P. paludosa and Sonneratia apetala while those of the winter were Brassica nigra, Coriandrum sativum, Zizyphus jujuba, Alstonia scholaris, E. globulus and Bruguiera gymnorrhiza. Very low (entrepreneurship. This should improve the socio-economic status of the people of this region.

  8. GROWTH AND ARCHITECTURE OF ROOT SYSTEMS OF PLANTS OF Eucalyptus camaldulensis, E. GRANDIS AND E. PELLITA WERE EVALUATED AFTER THE PLANTING

    Directory of Open Access Journals (Sweden)

    Paulo Sérgio dos Santos Leles

    2001-01-01

    Full Text Available The seedlings were produced according to two methodologies: pressed blocks and stiff plastic tubes. A mixture of decomposed sugar-cane bagasse (60% and industrial sugar-cane plant residues (40% were used as substrate. The blocks were made by pressing the humid substrate in metallic moulds with the dimensions of 60 x 40 x 20 cm (lenght, width and height and pressure of 10 kgf/cm2 for 15 minutes. Under this load the blocks height was reduced to 10 cm. They were placed in wooden boxes with screen bottom. The stiff plastic tubes had circular section 12 cm high, with volume capacity of 50 cm3. The seeds were sown in the blocks 5 cm apart, by means of a spatula. At the end of nursey phase, the seedlings were planted the field. After 2, 6 and 10 months of planting, the height and the diameter at the ground level were evaluated. At the age of 10 months it was also evaluated the number of laterals root and deformatiom cofficient the three plants for treatment. Two months after planting, the plants originated from the pressed blocks showed growth highly signifficant in relation to those of the stiff plastic tubes. At 10 months, of age only Eucalyptus grandis seedlings showed significant difference concerning to height and diameter at the ground level between plants produced by pressed blocks and stiff plastic tubes. For the three species, the pressed blocks seedling showed higher number of lateral roots and smaller number of root deformation coefficents in comparision to the plants from the stiff tubes.

  9. Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: a review.

    Science.gov (United States)

    Dhakad, Ashok K; Pandey, Vijay V; Beg, Sobia; Rawat, Janhvi M; Singh, Avtar

    2018-02-01

    The genus Eucalyptus L'Heritier comprises about 900 species, of which more than 300 species contain volatile essential oil in their leaves. About 20 species, within these, have a high content of 1,8-cineole (more than 70%), commercially used for the production of essential oils in the pharmaceutical and cosmetic industries. However, Eucalyptus is extensively planted for pulp, plywood and solid wood production, but its leaf aromatic oil has astounding widespread biological activities, including antimicrobial, antiseptic, antioxidant, chemotherapeutic, respiratory and gastrointestinal disorder treatment, wound healing, and insecticidal/insect repellent, herbicidal, acaricidal, nematicidal, and perfumes, soap making and grease remover. In the present review, we have made an attempt to congregate the biological ingredients of leaf essential oil, leaf oil as a natural medicine, and pharmacological and toxicological values of the leaf oil of different Eucalyptus species worldwide. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  10. Eucalypt NADP-Dependent Isocitrate Dehydrogenase1

    Science.gov (United States)

    Boiffin, Vincent; Hodges, Michael; Gálvez, Susana; Balestrini, Raffaella; Bonfante, Paola; Gadal, Pierre; Martin, Francis

    1998-01-01

    NADP-dependent isocitrate dehydrogenase (NADP-ICDH) activity is increased in roots of Eucalyptus globulus subsp. bicostata ex Maiden Kirkp. during colonization by the ectomycorrhizal fungus Pisolithus tinctorius Coker and Couch. To investigate the regulation of the enzyme expression, a cDNA (EgIcdh) encoding the NADP-ICDH was isolated from a cDNA library of E. globulus-P. tinctorius ectomycorrhizae. The putative polypeptide sequence of EgIcdh showed a high amino acid similarity with plant NADP-ICDHs. Because the deduced EgICDH protein lacks an amino-terminal targeting sequence and shows highest similarity to plant cytosolic ICDHs, it probably represents a cytoplasmic isoform. RNA analysis showed that the steady-state level of EgIcdh transcripts was enhanced nearly 2-fold in ectomycorrhizal roots compared with nonmycorrhizal roots. Increased accumulation of NADP-ICDH transcripts occurred as early as 2 d after contact and likely led to the observed increased enzyme activity. Indirect immunofluorescence microscopy indicated that NADP-ICDH was preferentially accumulated in the epidermis and stele parenchyma of nonmycorrhizal and ectomycorrhizal lateral roots. The putative role of cytosolic NADP-ICDH in ectomycorrhizae is discussed. PMID:9662536

  11. Screening for larvicidal activity of ethanolic and aqueous extracts of selected plants against Aedes aegypti and Aedes albopictus larvae

    Directory of Open Access Journals (Sweden)

    Michael Russelle Alvarez

    2016-02-01

    Full Text Available Objective: To screen for larvicidal activity of aqueous and ethanolic extracts (95% ethanol from Selaginella elmeri, Christella dentata, Elatostema sinnatum, Curculigo capitulata, Euphorbia hirta, Murraya koenigii (M. koenigii, Alpinia speciosa, Cymbopogon citratus, Eucalyptus globulus (E. globulus, Jatropha curcas (J. curcas, Psidium guajava, Gliricidia sepium, Ixora coccinea and Capsicum frutescens (C. frutescens against Aedes aegypti (A. aegypti and Aedes albopictus (A. albopictus 3rd instar larvae. Methods: Ethanolic and aqueous extracts were screened for larvicidal activity by exposing the A. aegypti and A. albopictus 3rd instar larvae (15 larvae per trial, triplicates for 48 h, counting the mortalities every 24 h. Additionally, phytochemical screening for flavonoids, tannins, alkaloids, anthraquinones, anthrones, coumarins, indoles and steroids were performed on active extracts using spray tests. Results: Against A. aegypti, the three most active extracts were C. frutescens ethanolic (100% after 24 and 48 h, J. curcas ethanolic (84.44% after 24 h and 88.89% after 48 h and M. koenigii ethanolic (53.33% after 24 h and 71.11% after 48 h. On the other hand, against A. albopictus, the three most active extracts were C. frutescens ethanolic (93.33% after 24 h and 100% after 48 h, J. curcas ethanolic (77.78% after 24 h and 82.22% after 48 h and E. globulus ethanolic (64.44% after 24 h and 73.33% after 48 h. Phytochemical screening was also performed on the active extracts, revealing alkaloids, tannins, indoles and steroids. Conclusios: The results demonstrate the larvicidal activities of ethanolic extracts of Cymbopogon citratus, Euphorbia hirta, Ixora coccinea, Gliricidia sepium, M. koenigii, E. globulus, J. curcas and C. frutescens against A. aegypti and A. albopictus 3rd instar larvae. These could be used as potential larvicidal agents for the control of these mosquitoes.

  12. COMBUSTION PROPERTIES OF EUCALYPTUS WOOD

    Directory of Open Access Journals (Sweden)

    Yalçın ÖRS

    1999-03-01

    Full Text Available In this study, the combustion properties of some impregnation materials (abiotic and biotic factors used for eucalyptus wood in interior or exterior environments were investigated. The experimental samples were prepared from Eucalyptus wood based on ASTM-D-1413-76 Tanalith-CBC, boric acid, borax, vacsol-WR, immersol-WR, polyethylen glycole-400 and ammonium sulphate were used as an impregnation material. The results indicated that, vacuum treatment on Eucalyptus gave the lowest retention value of salts. Compounds containing boron+salt increased fire resistance however water repellents decreased the wood flammability.

  13. EUCANEXT: an integrated database for the exploration of genomic and transcriptomic data from Eucalyptus species

    OpenAIRE

    Nascimento, Leandro Costa; Salazar, Marcela Mendes; Lepikson-Neto, Jorge; Camargo, Eduardo Leal Oliveira; Parreiras, Lucas Salera; Pereira, Gonçalo Amarante Guimarães; Carazzolle, Marcelo Falsarella

    2017-01-01

    Abstract Tree species of the genus Eucalyptus are the most valuable and widely planted hardwoods in the world. Given the economic importance of Eucalyptus trees, much effort has been made towards the generation of specimens with superior forestry properties that can deliver high-quality feedstocks, customized to the industrýs needs for both cellulosic (paper) and lignocellulosic biomass production. In line with these efforts, large sets of molecular data have been generated by several scienti...

  14. Glycine Betaine and Proline Production in Eucalyptus Plant under NaCl Harassing Environment

    International Nuclear Information System (INIS)

    Qureshi, T. M.; Bano, A.; Ashraf, M. Y.

    2015-01-01

    An investigation has been carried out to study the production of Proline and Betaine by applying Abscisic acid (ABA) treatment under NaCl and water stressed conditions. The seeds of four provenances of Eucalyptus camaldulesnis were obtained from the University of Agriculture, Faisalabad (Provenance I), Punjab Forest Research Institute, Faisalabad (Provenance II), Bio-saline Research Station-I, Lahore (Provenance III) and Bio-saline Research Station-II of Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad (Provenance 1V). It was observed that Proline and Betaine accumulation increased significantly in all the provenances with increase in drought or salt stress, ABA alone and in combination with drought. Provenance II and III species remained successful in maintaining higher Proline and Betaine accumulation as compared to Provenances I and IV. From the results it can be concluded that ABA treatment remains successful in enhancing Proline and Betaine production and maintaining the physiological parameters necessary to enhance plant growth both under salt and in combination with drought condition. (author)

  15. Provenance variation in growth and genetic potential of Aquilaria ...

    African Journals Online (AJOL)

    ARUN KUMAR

    Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam, Tamil Nadu, 641 301, India. Received 11 .... Author(s) agree that this article remains permanently open access under the terms of the Creative Commons Attribution License 4.0 ..... and chemical wood properties of Eucalyptus globulus.

  16. The gall wasp Leptocybe invasa (Hymenoptera: Eulophidae) stimulates different chemical and phytohormone responses in two Eucalyptus varieties that vary in susceptibility to galling.

    Science.gov (United States)

    Li, X Q; Liu, Y Z; Guo, W F; Solanki, M K; Yang, Z D; Xiang, Y; Ma, Z C; Wen, Y G

    2017-09-01

    Gall-inducing insects produce various types of galls on plants, but little is known about the gall-induction mechanism of these galling insects. The gall wasp Leptocybe invasa Fisher & LaSalle (Hymenoptera: Eulophidae) forms galls of different sizes on several Eucalyptus species. To clarify the physiological responses of Eucalyptus to L. invasa infestation, we measured the dynamics of nitrogen (N), carbon (C), total phenolics, total tannins and four types of phytohormones (zeatin [Z] + zeatin riboside [ZR], gibberellins [GA], indole-3-acetic acid [IAA] and abscisic acid [ABA]) in galled and ungalled leaf tissues of two Eucalyptus horticultural varieties (DH201-2 [Eucalyptus grandis × Eucalyptus camaldulensis] and EA [Eucalyptus exserta]) with different susceptibility to galling throughout the larval developmental stages. Nitrogen, total phenolics, tannins and four kinds of phytohormones strongly accumulated in tissues galled by L. invasa (especially during early larval feeding stages). While N, Z + ZR and GA levels were higher, tannins and ABA levels were lower in the galled tissues on the highly susceptible variety. Nitrogen, total phenolics, GA, Z + ZR and IAA levels in the galled tissues gradually decreased during gall development, but ABA and tannins conversely increased in the galled tissues of the less susceptible variety. Our results suggest that the effects of gall-inducing insects on plants depend not only on the susceptibility of the plant infested but also on the developmental stage of galled tissues. Gall formation process is thus synergistically influenced by both gall-inducing insect and plant genotypes. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  17. Effects of the interception of litterfall by the understory on carbon cycling in eucalyptus plantations of South China.

    Science.gov (United States)

    Yang, Long; Wang, Jun; Huang, Yuhui; Hui, Dafeng; Wen, Meili

    2014-01-01

    For the purposes of forest restoration, carbon (C) fixation, and economic improvement, eucalyptus (Eucalyptus urophylla) has been widely planted in South China. The understory of eucalyptus plantations is often occupied by a dense community of the fern Dicranopteris dichotoma, which intercepts tree canopy leaf litter before it reaches the ground. To understand the effects of this interception of litterfall on C cycling in eucalyptus plantations, we quantified the mass of intercepted litter and the influences of litterfall interception on litter decomposition and soil respiration. The total mass of E. urophylla litterfall collected on the understory was similar to that collected by the traditional litter trap method. All of the eucalyptus litterfall is intercepted by the D. dichotoma canopy. Of the litterfall that was intercepted by D. dichotoma, 20-40% and 60-80% was intercepted by the top (50-100 cm) and bottom (0-50 cm) of the understory canopy, respectively. Intercepted litterfall decomposed faster at the bottom of understory canopy (at the base of the plants) than at the top, and decomposition was slower on the soil surface in the absence of understory than on any location in the understory canopy. Soil respiration was highest when both the understory and litter were present and was lowest when both the understory and litter were absent. These results indicate that litterfall interception changed carbon flow between aboveground and belowground through litter decomposition and soil respiration, which changed carbon cycling in eucalyptus plantations. The effects of the understory on litter decomposition and soil respiration should be considered in ecosystem carbon models.

  18. Effects of the interception of litterfall by the understory on carbon cycling in eucalyptus plantations of South China.

    Directory of Open Access Journals (Sweden)

    Long Yang

    Full Text Available For the purposes of forest restoration, carbon (C fixation, and economic improvement, eucalyptus (Eucalyptus urophylla has been widely planted in South China. The understory of eucalyptus plantations is often occupied by a dense community of the fern Dicranopteris dichotoma, which intercepts tree canopy leaf litter before it reaches the ground. To understand the effects of this interception of litterfall on C cycling in eucalyptus plantations, we quantified the mass of intercepted litter and the influences of litterfall interception on litter decomposition and soil respiration. The total mass of E. urophylla litterfall collected on the understory was similar to that collected by the traditional litter trap method. All of the eucalyptus litterfall is intercepted by the D. dichotoma canopy. Of the litterfall that was intercepted by D. dichotoma, 20-40% and 60-80% was intercepted by the top (50-100 cm and bottom (0-50 cm of the understory canopy, respectively. Intercepted litterfall decomposed faster at the bottom of understory canopy (at the base of the plants than at the top, and decomposition was slower on the soil surface in the absence of understory than on any location in the understory canopy. Soil respiration was highest when both the understory and litter were present and was lowest when both the understory and litter were absent. These results indicate that litterfall interception changed carbon flow between aboveground and belowground through litter decomposition and soil respiration, which changed carbon cycling in eucalyptus plantations. The effects of the understory on litter decomposition and soil respiration should be considered in ecosystem carbon models.

  19. Manganese accumulation and its relation to "eucalyptus shoot blight in the Vale do Rio Doce"

    Directory of Open Access Journals (Sweden)

    Fernando Palha Leite

    2014-02-01

    Full Text Available Eucalyptus Shoot Blight in the Vale do Rio Doce (ESBVRD is an anomaly that leads to reduced growth and, in more extreme cases, to death of eucalyptus plants. Initially diagnosed in plantations in the region of the Vale do Rio Doce, in the State of Minas Gerais, Brazil, this problem has also been found in plantations in other regions of the country and even in other countries. Although the symptoms of this anomaly are well-known, its causes are not yet understood. The aim of this study was to evaluate the cause-effect relationship between accumulation of manganese (Mn in eucalyptus clones and ESBVRD. Characterization of the environment in areas of greater occurrence of this problem in regard to soil, climate and fluctuation of the water table was undertaken in eucalyptus plantations of the Celulose Nipo-brasileira S.A. (Cenibra company in the region of the Vale do Rio Doce. Plant tissues were sampled in two situations. In the first situation, diagnosis occurred in the initial phase of the anomaly in clones with differentiated tolerance to the problem; in the second situation, diagnosis was made in a single clone, considered to be sensitive, in two time periods - in the phase with the strong presence of symptoms and in the recovery phase, in areas of occurrence and in areas of escape from the problem. The most ESBVRD-sensitive clone showed much higher (4.8 times higher leaf Mn contents than more tolerant clones. In plants with the anomaly, Mn leaf contents were greater than 3,070 mg kg-1, much greater than the quantity found in those without the anomaly (734 mg kg-1. In the period in which the symptoms began to wane, there was a sharp decline in leaf Mn contents, from 2,194 to 847 mg kg-1. Manganese content in the above ground part and plant litter (44.4 g ha-1 in the area of occurrence of the anomaly was three times greater than that found in these same components (14.1 g ha-1 in the area of absence of the symptom. Based on the evidence found

  20. QUALIDADE DA CELULOSE KRAFT-ANTRAQUINONA DE Eucalyptus dunnii PLANTADO EM CINCO ESPAÇAMENTOS EM RELAÇÃO AO Eucalyptus grandis E Eucalyptus saligna

    OpenAIRE

    Maria Cladis Mezzomo da Silva; Edvins Ratnieks; Teotônio Francisco de Assis; Celso Edmundo B. Foelkel; Jorge Vieira Gonzaga; Giovanni Willer Ferreira

    1997-01-01

    Este trabalho analisou o efeito de cinco espaçamentos de Eucalyptus dunnii (3 m x 1 m; 3 m x 1,5 m; 3 m x 2 m; 3 m x 3 m; 3 m x 4 m), para produção de celulose kraft-antraquinona, comparados com Eucalyptus grandis e Eucalyptus saligna (ambos no espaçamento 3 m x 2 m). Avaliaram-se a densidade básica e composição química da madeira original, analisando-se extrativos em diclorometano, pentosanas, lignina, solubilidade em NaOH8% e cinzas. Os cavacos foram submetidos a cozimento kraft-antraquinon...

  1. Neuroprotective and Anti-Aging Potentials of Essential Oils from Aromatic and Medicinal Plants.

    Science.gov (United States)

    Ayaz, Muhammad; Sadiq, Abdul; Junaid, Muhammad; Ullah, Farhat; Subhan, Fazal; Ahmed, Jawad

    2017-01-01

    The use of essential oils (EOs) and their components is known since long in traditional medicine and aromatherapy for the management of various diseases, and is further increased in the recent times. The neuroprotective and anti-aging potentials of EOs and their possible mechanism of actions were evaluated by numerous researchers around the globe. Several clinically important EOs and their components from Nigella sativa , Acorus gramineus, Lavandula angustifolia, Eucalyptus globulus, Mentha piperita, Rosmarinus officinalis, Jasminum sambac, Piper nigrum and so many other plants are reported for neuroprotective effects. This review article was aimed to summarize the current finding on EOs tested against neurodegenerative disorders like Alzheimer disease (AD) and dementia. The effects of EOs on pathological targets of AD and dementia including amyloid deposition (Aβ), neurofibrillary tangles (NFTs), cholinergic hypofunction, oxidative stress and glutamatergic abnormalities were focused. Furthermore, effects of EOs on other neurological disorders including anxiety, depression, cognitive hypofunction epilepsy and convulsions were also evaluated in detail. In conclusion, EOs were effective on several pathological targets and have improved cognitive performance in animal models and human subjects. Thus, EOs can be developed as multi-potent agents against neurological disorders with better efficacy, safety and cost effectiveness.

  2. Response of Eucalyptus occidentais to water stress induced by NaCl

    Energy Technology Data Exchange (ETDEWEB)

    Itai, C.

    1978-01-01

    Eucalyptus occidentalis plants were exposed to NaCl in their culture solution for various time. Determination of cytokinin, abscisic acid, /sup 14/C leucine incorporation of E. occidentalis, a xerophyte, does not differ from the response of mesophytes to such treatments. 13 references, 1 figure, 4 tables.

  3. Aspectos biológicos de Thyrinteina arnobia (Lep.: Geometriadae provenientes de lagartas criadas em folhas de Eucalyptus cloeziana ou de Psidium guajava sob condições de campo Biological aspects of Thyrinteina arnobia (Lep.: Geometridae adults originated from caterpillars reared on leaves of Eucalyptus cloeziana or Psidium guajava under field conditions

    Directory of Open Access Journals (Sweden)

    Anderson Mathias Holtz

    2003-12-01

    Full Text Available Os plantios de Eucalyptus no Brasil podem sofrer danos por espécies nativas de insetos de diversas ordens, como Orthoptera, Coleoptera e Lepidoptera. Esses insetos podem alimentar-se tanto de mirtáceas brasileiras como goiabeira, gabirobeira, jabuticabeira, entre outras, como de espécies do gênero Eucalyptus. Entre os desfolhadores, destaca-se Thyrinteina arnobia (Stoll (Lepidoptera: Geometridae como o mais daninho dessa ordem para a eucaliptocultura brasileira. Este trabalho teve por objetivo avaliar aspectos biológicos de adultos de T. arnobia provenientes de lagartas alimentadas com plantas de eucalipto e, ou, goiabeira. Adultos deste inseto criados em folhas de eucalipto e, ou, de goiabeira apresentaram diferenças significativas para a maioria dos aspectos biológicos avaliados, exceto para a duração dos períodos de préoviposição, de oviposição e razão sexual. Assim, insetos herbívoros que vivem em hospedeiros filogeneticamente próximos ao eucalipto são capazes de causar danos consideráveis em reflorestamentos com espécies desse grupo, o que provavelmente ocorre pelo fato de elas estarem ainda em processo de adaptação a essa praga que atacaria o eucalipto, por estar fugindo da pressão exercida por barreiras físicas e químicas existentes nas mirtáceas nativas brasileiras.Eucalyptus plantations in Brazil may be damaged by native insects of many orders including Orthoptera, Coleoptera and Lepidoptera. These insects feed on Brazilian tree species of the family Myrtaceae to which the genus Eucalyptus belongs. The Lepidoptera Thyrinteina arnobia Stoll (Lepidoptera: Geometridae is the most harmful defoliator of Eucalyptus in Brazil. The objective of this work was to evaluate biological aspects of T. arnobia adults originated from caterpillars fed on guava or eucalyptus plants. Adults of T. arnobia originated from caterpillars reared with eucalyptus or guava leaves presented significant differences for most biological

  4. Determination of chemical elements in Eucalyptus grandis, manured with Ballad's, by neutrons activation analysis

    International Nuclear Information System (INIS)

    Mateus, Natalina de Fatima; Madi Filho, Tufic

    2007-01-01

    The biosolid is a mud resulting from the biological treatment of wasted liquids. It is considered as a profitable alternative and important to minimize the environmental impact generated by the sewage thrown in to sanitary lands, in forest cultures like the Eucalyptus grandis. The objective of this work was to detect which chemical elements are present in Eucalyptus grandis samples, fertilized with different quantities of biosolid. The eucalyptuses of Estacao Experimental de Ciencias Florestais of Itatinga were planted in March of 1998 and collected with five years old. The used biosolid was produced by Station of Treatment of Sewer of Barueri - SP, classified as kind B. For the determination of the presence and quantity of chemical elements in the eucalyptus samples, an analysis technique by neutronic activation (NAA) was used followed by gamma rays spectroscopy. The samples were irradiated in the Nuclear Reactor IEA-R1 of IPEN-SP, followed by the measure of induced gamma rays activity, using a Detector HPGe. The presence, mainly of Br, Mn, Na and K, was detected in all analyzed samples. (author)

  5. Pre-Infection Stages of Austropuccinia psidii in the Epidermis of Eucalyptus Hybrid Leaves with Different Resistance Levels

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    Renata Ruiz Silva

    2017-10-01

    Full Text Available Rust is a major Eucalyptus spp. disease, which is especially damaging for early-stage plants. The aim of this study was to verify the pre-infection process of Austropuccinia psidii (A. psidii in the leaves of three phenological stages of Eucalyptus clones with different resistance levels. Plants from the hybrids of Eucalyptus urophylla × Eucalyptus grandis (E. grandis with variable levels of resistance to this disease were used. The pathogen was inoculated in vitro on abaxial leaf discs of first, third, and fifth leaf stages and maintained under conditions suitable for disease development. Subsequently, samples from these discs were collected 24 and 120 h after inoculation and processed using scanning electron microscopy analysis. No symptoms were seen in any leaf stage of the resistant clone. Additionally, a low incidence of A. psidii germination (1.3–2% and appressoria (0–0.5% in three leaf stages was observed. However, the first leaf stage of the susceptible clone presented germination of large numbers of urediniospores (65% with appressoria (55% and degradation of the cuticle and wax. From the third stage, the percentage of germinated urediniospores (<15% and appressoria (<2% formation of this clone decreased. Protrusions on the leaf surface, associated with the pathogen, were observed on the first and third leaf stages of the resistant clone and on the fifth stage of the susceptible clone, suggesting a possible defensive plant reaction.

  6. Avaliação in vitro da atividade antifúngica de extratos de plantas e óleo de eucalipto sobre Trichophyton mentagrophytes In vitro evaluation of the antifungal activity of plant extracts and eucalyptus oil on Trichophyton mentagrophytes

    Directory of Open Access Journals (Sweden)

    D.F.R. Frias

    2009-01-01

    Full Text Available O presente estudo teve como objetivo determinar a ação antifúngica de extratos de plantas medicinais e óleo de eucalipto frente ao dermatófito Trichophyton mentagropytes, visando a utilização da fitoterapia no controle. As plantas utilizadas na obtenção dos extratos foram arruda (Ruta graveolens, citronela (Cymbopogon nardus, cravo de defunto (Tagetes minuta, eucalipto (Eucalyptus spp, graviola (Annona muricata, fruta do conde (Annona spp, manga (Mangifera indica, romã (Punica granatum, flores e folhas de primavera (Bougainvillea spectabilis. Verificou-se que uso de 0,5% óleo de eucalipto no combate ao T. mentagropytes foi eficaz, já os extratos de citronela (4% eucalipto (5% e romã (8% atuaram como fungistáticos e os restantes não devem ser usados contra este dermatófito porque não causaram nenhum efeito.The aim of this study was to assess the antifungal action of medicinal plant extracts and eucalyptus oil against the dermatophyte Trichophyton mentagrophytes in order to employ phytotherapy for its control. The plants used for extract production were common rue (Ruta graveolens, citronella (Cymbopogon nardus, wild marigold (Tagetes minuta, eucalyptus (Eucalyptus spp, sweetsop (Annona muricata, custard apple (Annona spp, mango (Mangifera indica, pomegranate (Punica granatum, besides flowers and leaves of bougainvillea (Bougainvillea spectabilis. The use of 0.5% eucalyptus oil was effective in controlling Trichophyton mentagrophytes; however, citronella (4%, eucalyptus (5% and pomegranate (8% extracts acted as fungistatic, and the remaining extracts should not be used against this dermatophyte since they did not have any effect.

  7. Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus

    Directory of Open Access Journals (Sweden)

    Tian-Min Qiao

    2016-10-01

    Full Text Available Eucalyptus dieback disease, caused by Cylindrocladium scoparium, has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP were developed for detection of C. scoparium based on factor 1-alpha (tef1 and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium. The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products.

  8. Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus

    Science.gov (United States)

    Qiao, Tian-Min; Zhang, Jing; Li, Shu-Jiang; Han, Shan; Zhu, Tian-Hui

    2016-01-01

    Eucalyptus dieback disease, caused by Cylindrocladium scoparium, has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium. The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products. PMID:27721691

  9. Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus.

    Science.gov (United States)

    Qiao, Tian-Min; Zhang, Jing; Li, Shu-Jiang; Han, Shan; Zhu, Tian-Hui

    2016-10-01

    Eucalyptus dieback disease, caused by Cylindrocladium scoparium , has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium . The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products.

  10. Wood Permeability in Eucalyptus grandis and Eucalyptus dunnii

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    Raphael Nogueira Rezende

    2017-12-01

    Full Text Available ABSTRACT The objective of this study was to evaluate the flow of air and water in Eucalyptus grandis and Eucalyptus dunnii wood. Wood was collected from four trees aged 37 years in an experimental plantation of the Federal University of Lavras, Brazil. Planks were cut off the basal logs to produce specimens for air and water permeability testing. Results indicated that the longitudinal permeability to air and water of E. grandis wood were, on average, 5% and 10% higher, respectively, than that of E. dunnii wood. E. grandis and E. dunnii wood showed neither air nor water flow in the test for permeability transversal to the fibers, and longitudinal permeability to air exceeded that to water by approximately 50 fold in both species.

  11. ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OIL OF Myrtus ...

    African Journals Online (AJOL)

    2015-02-05

    Feb 5, 2015 ... The essential oil of M communis leaves, growing wild in Iran contains ... The antibacterial properties of the essential oils of myrtle leaves and extracts .... of nutrients, temperature, humidity, soil type, day length, climate, .... composition and antimicrobial effects of essential oils of Eucalyptus globulus, Myrtus.

  12. Advances in reproductive biology and seed production systems of ...

    African Journals Online (AJOL)

    Eucalyptus globulus is the main eucalypt species grown in Australian plantations. The focus on seedling deployment systems, coupled with exploitation of large, open-pollinated base populations for breeding purposes over the last two decades, has required a detailed understanding of the reproductive biology of this ...

  13. Initial analysis of the Eucalyptus super clone fertilized with swine wastewater sludge

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    Elaino Paula Ribeiro

    2015-12-01

    Full Text Available The generation of waste has gradually increased due to the large swine production and with this comes the concern about the final destination of the product. This study aimed to analyze the initial behavior of Eucalyptus super clone (AEC 1528, fertilized with swine sludge and compared to chemical fertilizer. The experiment was conducted in a completely randomized design with 5 treatments and 3 replications, with the volumes: 1.25, 2.5, 5 liters; 0 liters of sewage sludge and chemical fertilizer. The plants were irrigated with water from a well at the Rio Verde University, increasing the amount according to plant growth. After 120 days, we evaluated the height of the plants, stem diameter, relation between stem diameterand plantsheight, weight of the fresh matter, weight of the dry matter,root weight, leaf areaand mortality. There were significant differences in relation to plant height, the fresh matterweightand the dry matterweight. For the other characteristics there was no negative influence with the use of  different volumes of sewage sludge in eucalypts hybrid. It was observedthat, the application of biosolids (sludge from swine is an effective alternative fertilizer in eucalyptus plantations.

  14. TANNIN CONTENT DETERMINATION IN THE BARK OF Eucalyptus spp

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    Paulo Fernando Trugilho

    2003-07-01

    Full Text Available The objective of this study was to determine the tannin contents in the bark oftwenty-five species of Eucalyptus through two extraction methods, one using hot water andthe other a sequence of toluene and ethanol. The results showed that the extraction methodspresented significant differences in the tannin contents. The method using the sequencetoluene and ethanol, for most of the species, promoted a larger extraction of tannin. The hotwater method presented higher contents of tannin for Eucalyptus cloeziana (40,31%,Eucalyptus melanophoia (20,49% and Eucalyptus paniculata (16,03%. In the toluene andethanol method the species with higher tannin content was Eucalyptus cloeziana (31,00%,Eucalyptus tereticornis (22,83% and Eucalyptus paniculata (17,64%. The Eucalyptuscloeziana presented great potential as commercial source of tannin, independent of theextraction method considered.

  15. Ethnobotanical study of the medicinal plants from Tlanchinol, Hidalgo, México.

    Science.gov (United States)

    Andrade-Cetto, Adolfo

    2009-02-25

    The people in Mexico still depend upon the use of medicinal plants to treat simple health problems, including those who live in regions like Tlanchinol Hidalgo, where it is still possible to find people who speak the pre-Hispanic Nahua language. This area is surrounded by rain forest, which is more or less well conserved, so ethnopharmacological field studies are quite relevant. The cultural knowledge about the use of medicinal plants converge with the richness in the surrounding flora making this region ideal for the selection of traditionally used medicinal plants. To present the results of an ethnopharmacological field survey conducted in the municipality of Tlanchinol Hidalgo, Mexico analyzed with two different quantitative tools, with the aim of selecting the most important species used in traditional medicine. Direct interviews with the people were performed in several short visits to the municipality of Tlanchinol Hidalgo. The plants were collected, and the species were determined. The interviews were analyzed with two quantitative tools. First, the factor informant consensus highlighted the agreement in the use of plants and the fidelity level defined as: the ratio between the number of informants who independently suggested the use of a species for the same major purpose and the total number of informants who mentioned the plant for any use. Furthermore, we analyzed the use-mentions for the plants. The results of the factor informant consensus showed that the gastrointestinal category had the greatest agreement, followed by the respiratory and dermatological categories. The most important species according to their fidelity are: Coleus blumei, Plantago australis and Lippia dulcis for the gastrointestinal category; Borago officinalis, Foeniculum vulgare, and Eucalyptus globulus for the respiratory category; and Ageratum houstonianum and Solanum nigrescens for the dermatological category. As a result of the present study, we recommend the plants listed in

  16. Supply chain optimization of sugarcane first generation and eucalyptus second generation ethanol production in Brazil

    International Nuclear Information System (INIS)

    Jonker, J.G.G.; Junginger, H.M.; Verstegen, J.A.; Lin, T.; Rodríguez, L.F.; Ting, K.C.; Faaij, A.P.C.; Hilst, F. van der

    2016-01-01

    Highlights: • Optimal location & scale of ethanol plants for expansion in Goiás until 2030. • Ethanol costs from sugarcane vary between 710 and 752 US$/m"3 in 2030. • For eucalyptus-based ethanol production costs vary between 543 and 560 US$/m"3 in 2030. • System-wide optimization has a marginal impact on overall production costs. • The overall GHG emission intensity is mainly impacted by former land use. - Abstract: The expansion of the ethanol industry in Brazil faces two important challenges: to reduce total ethanol production costs and to limit the greenhouse gas (GHG) emission intensity of the ethanol produced. The objective of this study is to economically optimize the scale and location of ethanol production plants given the expected expansion of biomass supply regions. A linear optimization model is utilized to determine the optimal location and scale of sugarcane and eucalyptus industrial processing plants given the projected spatial distribution of the expansion of biomass production in the state of Goiás between 2012 and 2030. Three expansion approaches evaluated the impact on ethanol production costs of expanding an existing industry in one time step (one-step), or multiple time steps (multi-step), or constructing a newly emerging ethanol industry in Goiás (greenfield). In addition, the GHG emission intensity of the optimized ethanol supply chains are calculated. Under the three expansion approaches, the total ethanol production costs of sugarcane ethanol decrease from 894 US$/m"3 ethanol in 2015 to 752, 715, and 710 US$/m"3 ethanol in 2030 for the multi-step, one step and greenfield expansion respectively. For eucalyptus, ethanol production costs decrease from 635 US$/m"3 in 2015 to 560 and 543 US$/m"3 in 2030 for the multi-step and one-step approach. A general trend is the use of large scale industrial processing plants, especially towards 2030 due to increased biomass supply. We conclude that a system-wide optimization as a marginal

  17. Can the Understory Affect the Hymenoptera Parasitoids in a Eucalyptus Plantation?

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    Onice Teresinha Dall'Oglio

    Full Text Available The understory in forest plantations can increase richness and diversity of natural enemies due to greater plant species richness. The objective of this study was to test the hypothesis that the presence of the understory and climatic season in the region (wet or dry can increase the richness and abundance of Hymenoptera parasitoids in Eucalyptus plantations, in the municipality of Belo Oriente, Minas Gerais State, Brazil. In each eucalyptus cultivation (five areas of cultivation ten Malaise traps were installed, five with the understory and five without it. A total of 9,639 individuals from 30 families of the Hymenoptera parasitoids were collected, with Mymaridae, Scelionidae, Encyrtidae and Braconidae being the most collected ones with 4,934, 1,212, 619 and 612 individuals, respectively. The eucalyptus stands with and without the understory showed percentage of individuals 45.65% and 54.35% collected, respectively. The understory did not represent a positive effect on the overall abundance of the individuals Hymenoptera in the E. grandis stands, but rather exerted a positive effect on the specific families of the parasitoids of this order.

  18. Can the Understory Affect the Hymenoptera Parasitoids in a Eucalyptus Plantation?

    Science.gov (United States)

    Dall’Oglio, Onice Teresinha; Ribeiro, Rafael Coelho; Ramalho, Francisco de Souza; Fernandes, Flávio Lemes; Wilcken, Carlos Frederico; de Assis Júnior, Sebastião Lourenço; Rueda, Rosa Angélica Plata; Serrão, José Eduardo; Zanuncio, José Cola

    2016-01-01

    The understory in forest plantations can increase richness and diversity of natural enemies due to greater plant species richness. The objective of this study was to test the hypothesis that the presence of the understory and climatic season in the region (wet or dry) can increase the richness and abundance of Hymenoptera parasitoids in Eucalyptus plantations, in the municipality of Belo Oriente, Minas Gerais State, Brazil. In each eucalyptus cultivation (five areas of cultivation) ten Malaise traps were installed, five with the understory and five without it. A total of 9,639 individuals from 30 families of the Hymenoptera parasitoids were collected, with Mymaridae, Scelionidae, Encyrtidae and Braconidae being the most collected ones with 4,934, 1,212, 619 and 612 individuals, respectively. The eucalyptus stands with and without the understory showed percentage of individuals 45.65% and 54.35% collected, respectively. The understory did not represent a positive effect on the overall abundance of the individuals Hymenoptera in the E. grandis stands, but rather exerted a positive effect on the specific families of the parasitoids of this order. PMID:26954578

  19. Early selection of Eucalyptus clones in retrospective nursery test ...

    African Journals Online (AJOL)

    Within the framework of the eucalyptus breeding programme in the Congo, two retrospective tests were conducted using mature clones in the field and young cuttings under nursery conditions with two hybrids: 13 clones of Eucalyptus tereticornis* Eucalyptus grandis for the test TC 82-1B and 17 clones of Eucalyptus ...

  20. Effects of Allelochemicals of Some Eucalyptus Species on ...

    African Journals Online (AJOL)

    A laboratory experiment was conducted to assess the effects of allelochemicals of Eucalyptus camaldulensis, Eucalyptus citriodora and Eucalyptus globules on germination and root elongation using leguminous crop ground nut (Arachis hypogea) as bioassay material. The experiments were conducted in sterilized ...

  1. Botanical, Phytochemical, and Anticancer Properties of the Eucalyptus Species.

    Science.gov (United States)

    Vuong, Quan V; Chalmers, Anita C; Jyoti Bhuyan, Deep; Bowyer, Michael C; Scarlett, Christopher J

    2015-06-01

    The genus Eucalyptus (Myrtaceae) is mainly native to Australia; however, some species are now distributed globally. Eucalyptus has been used in indigenous Australian medicines for the treatment of a range of aliments including colds, flu, fever, muscular aches, sores, internal pains, and inflammation. Eucalyptus oils containing volatile compounds have been widely used in the pharmaceutical and cosmetics industries for a multitude of purposes. In addition, Eucalyptus extracts containing nonvolatile compounds are also an important source of key bioactive compounds, and several studies have linked Eucalyptus extracts with anticancer properties. With the increasing research interest in Eucalyptus and its health properties, this review briefly outlines the botanical features of Eucalyptus, discusses its traditional use as medicine, and comprehensively reviews its phytochemical and anticancer properties and, finally, proposes trends for future studies. Copyright © 2015 Verlag Helvetica Chimica Acta AG, Zürich.

  2. Hardening of eucalyptus seedlings via salicylic acid application

    Directory of Open Access Journals (Sweden)

    Eduardo Henrique Lima Mazzuchelli

    2014-09-01

    Full Text Available The agricultural and forest productivity suffer restrictions imposed by water stress, high temperature and high solar radiation. This study aimed to evaluate the capacity of stress attenuation and growth promotion of salicylic acid (SA application in eucalyptus (E. urophylla x E. grandis hybrid seedlings under water stress. A completely randomized design, in a 3x4 factorial scheme (three water treatments: constant irrigation with daily replacement of 40% (CI40% or 100% (CI100% of evapotranspirated water, and temporary irrigation suspension with replacement of only 40% of evapotranspirated water (S40%; and four SA concentrations: 0 mg L-1, 100 mg L-1, 200 mg L-1 and 300 mg L-1, was used. Plant photosynthetic parameters and biometric features were evaluated. The stomatal limitation was higher in plants under S40% irrigation, however, the SA application reverted this result, allowing the maintenance of the photosynthetic potential. There was interaction between irrigation regimes and SA doses for number of leaves, leaf area/number of leaves ratio and shoot and root dry mass. It was concluded that the application of 200 mg L -1 of SA positively affected the growth of eucalyptus seedlings under water stress, being considered an auxiliary management technique to their hardening process.

  3. Growth and yield models for Eucalyptus grandis grown in Swaziland ...

    African Journals Online (AJOL)

    The aim of this study was to develop a stand-level growth and yield model for short-rotationEucalyptus grandis grown for pulp wood production at Piggs Peak in Swaziland. The data were derived from a Nelder 1a spacing trial established with E. grandis clonal cuttings in 1998 and terminated in 2005. Planting density ...

  4. Experimental alternatives for evaluation of progenies and clones in eucalyptus breeding programs

    Directory of Open Access Journals (Sweden)

    Souza Elaine Aparecida de

    2003-01-01

    Full Text Available The feasibility of using augmented block designs and spatial analysis methods for early stage selection in eucalyptus breeding programs was tested. A total of 113 half-sib progenies of Eucalyptus urophylla and eight clones were evaluated in an 11 x 11 triple lattice experiment at two locations: Posto da Mata (Bahia, Brazil and São Mateus (Minas Gerais, Brazil. Four checks were randomly allocated within each block. Plots consisted of 15 m long rows containing 6 plants spaced 3 m apart. The girth at breast height (cm/plant was evaluated at 19 and 26 months of age. Variance analyses were performed according to the following methods: lattice design, randomized complete block design, augmented block design, Papadakis method, moving means method, and check plots. Comparisons among different methods were based on the magnitude of experimental errors and precision of the estimates of genetic and phenotypic parameters. General results indicated that augmented block design is useful to evaluate progenies and clones in early selection in eucalyptus breeding programs using moderate and low selection intensities. However, this design is not suitable for estimating genetic and phenotypic parameters due to its low precision. Check plots, nearest neighbour, Papadakis (1937, and moving means methods were efficient in removing the heterogeneity within blocks. These efficiencies were compared to that in lattice analysis for estimation of genetic and phenotypic parameters.

  5. Characterization of diazotrophic bacteria non-symbiotic associated with eucalyptus (eucalyptus sp.) in Codazzi, Cesar (Colombia)

    International Nuclear Information System (INIS)

    Obando Castellanos, Dolly Melissa; Burgos Zabala, Ludy Beatriz; Rivera Botia, Diego Mauricio; Rubiano Garrido, Maria Fernanda; Divan Baldini, Vera Lucia; Bonilla Buitrago, Ruth Rebeca

    2010-01-01

    The effect of climatic seasons (rainy and dry) and the stratum sample (rhizospheric soil, roots and leaves) the population of the genera Azotobacter, Beijerinckia, Derxia, Azospirillum, Herbaspirillum, Gluconacetobacter and Burkholderia in soil rhizosphere, roots and leaves of eucalyptus (eucalyptus sp.). It also assesses their ability to produce indoles compounds as plant growth promoters and their acetylene reduction activity as an indicator of biological fixation of nitrogen. The results showed no statistically significant differences in the Duncan test (p ≤ 0.05) in the population with respect to the climate epoch, suggesting that these bacteria are able to tolerate stress conditions by different physiological mechanisms. With respect to the stratum sample isolates attempts of Herbaspirillum sp. and Azospirillum sp. significant differences in rhizospheric soil and roots. we obtained 44 isolates of which were grouped by phenotypic characterization as 14 suspected of Beijerinckia sp., 12 Azotobacter sp., 8 Derxia sp., 4 Herbaspirillum sp., 5 Azospirillum sp., 1 Gluconacetobacter sp. and 1 Burkholderia sp. due to their high potential were selected isolates C27, C26 and C25. These four strains present the best values of efficiency in vitro, exceeding production values of the reference strains used (A. chroococcum (AC1) and a. brasilense (SP7)).

  6. DETERMINACIÓN DE MEDIOS DE CULTIVO Y PH PARA LA MASIFICACIÓN in vitro DE CEPAS DE Suillus luteus Aubl. ASOCIADAS A Pinus radiata D. Don Y Scleroderma citrinum Pers. ASOCIADAS A Eucalyptus globulus Labill. DE LA REGIÓN DEL BIOBÍO, CHILE

    Directory of Open Access Journals (Sweden)

    Marta González

    2015-02-01

    Full Text Available El uso de agentes biológicos en la producción en vivero ha aflorado como un elemento que permite no sólo mejorar la calidad morfológica y fisiológica de las plantas, sino que también posibilitar un mayor éxito en el ámbito silvicultural de una plantación. Estos agentes biológicos requieren de un ambiente óptimo para crecer, asociarse y reproducirse. El objetivo de este estudio fue determinar la dependencia del pH y el medio de cultivo en el crecimiento in vitro de distintas cepas de Suillus luteus y Scleroderma citrinum asociadas a Pinus radiata y Eucalyptus globulus, respectivamente. El estudio se realizó en condiciones controladas de temperatura y humedad, disponiendo los inóculos en placas Petri con diferentes medios cultivo, evaluando parámetros de crecimiento y biomasa a los 38 días para S. luteus y 105 días para S. citrinum. Los resultados indican que tanto el medio de cultivo, el pH del medio, así como las cepas de cada especie estudiada, son determinantes en las respuestas de crecimiento de los hongos ectomicorrícicos in vitro evaluados. Las cepas de S. luteus se desarrollaron adecuadamente en un medio de cultivo con abundancia de nutrientes (BAF, MMN como en baja presencia de ellos (EMA, y con pH más bien ácido (4,8 y 5,8. Por otro lado, las cepas de S. citrinum presentaron, para los mismos ambientes, un desarrollo inferior y lento, no obstante, la cepa Sc8 se reprodujo de forma óptima y rápida bajo un medio de cultivo BAF y con un pH moderadamente ácido de 5,8.

  7. Afforestation alters community structure of soil fungi.

    Science.gov (United States)

    Carson, Jennifer K; Gleeson, Deirdre B; Clipson, Nicholas; Murphy, Daniel V

    2010-07-01

    Relatively little is known about the effect of afforestation on soil fungal communities. This study demonstrated that afforestation altered fungal community structure and that changes were correlated to pools of soil C. Pasture at three locations on the same soil type was afforested with Eucalyptus globulus or Pinus pinaster. The structure of fungal communities under the three land uses was measured after 13y using automated ribosomal intergenic spacer analysis (ARISA). Afforestation significantly altered the structure of fungal communities. The effect of location on the structure of fungal communities was limited to pasture soils; although these contained the same plant species, the relative composition of each species varied between locations. Differences in the structure of fungal communities between pasture, E. globulus and P. pinaster were significantly correlated with changes in the amount of total organic C and microbial biomass-C in soil. Afforestation of patches of agricultural land may contribute to conserving soil fungi in agricultural landscapes by supporting fungal communities with different composition to agricultural soils. Copyright © 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

  8. Polyester composites reinforced with corona-treated fibers from pine, eucalyptus and sugarcane bagasse

    Science.gov (United States)

    This study aims to evaluate plant fibers that were surface activated with NaOH and corona discharge before incorporating in ortho unsaturated polyester-based fiber composites. It demonstrates the potential use of lignocellulosic particles, especially eucalyptus that presented the higher values for a...

  9. QUALIDADE DA CELULOSE KRAFT-ANTRAQUINONA DE Eucalyptus dunnii PLANTADO EM CINCO ESPAÇAMENTOS EM RELAÇÃO AO Eucalyptus grandis E Eucalyptus saligna

    Directory of Open Access Journals (Sweden)

    Maria Cladis Mezzomo da Silva

    1997-12-01

    Full Text Available Este trabalho analisou o efeito de cinco espaçamentos de Eucalyptus dunnii (3 m x 1 m; 3 m x 1,5 m; 3 m x 2 m; 3 m x 3 m; 3 m x 4 m, para produção de celulose kraft-antraquinona, comparados com Eucalyptus grandis e Eucalyptus saligna (ambos no espaçamento 3 m x 2 m. Avaliaram-se a densidade básica e composição química da madeira original, analisando-se extrativos em diclorometano, pentosanas, lignina, solubilidade em NaOH8% e cinzas. Os cavacos foram submetidos a cozimento kraft-antraquinona com álcali ativo variando de 18,5 a 21% para obter um número kappa 17± 1,5; sulfidez a 8%, antraquinona base madeira 0,05%, relação licor/madeira 4:1, tempo até temperatura máxima 60 minutos, tempo à temperatura máxima 45 minutos. A celulose marrom resultante do cozimento kraft-antraquinona foi caracterizada e analisada em seus aspectos físico-químicos (rendimentos, rejeitos, número kappa, viscosidade intrínseca, alvura e solubilidade em NaOH5%. Uma parte desta sofreu refinação em moinho Jökro ao nível de 35°SR e, juntamente com a polpa não-refinada, foram submetidas a testes físico-mecânicos e óticos (resistência à tração, alongamento, estouro, rasgo, volume específico, alvura, resistência ao ar Gurley e ascensão capilar Klemm. O maior consumo de álcali ativo (20,5% ocorreu em espaçamento 3 m x 4 m, proporcionando maior degradação da polpa. Todos os espaçamentos caracterizados apresentaram viabilidade para a produção de celulose kraft-antraquinona, podendo proporcionar redução nos custos de produção de celulose, devido aos menores consumos apresentados de álcali ativo e madeira quando comparados com Eucalyptus grandis e Eucalyptus saligna. Os valores de extrativos e cinzas mostraram-se elevados para Eucalyptus dunnii, potencializando possíveis impactos em termos de incrustações e fechamento de circuito no processo fabril. Diante dos resultados obtidos, a celulose de Eucalyptus dunnii atende às exig

  10. Wood anatomical and chemical properties related to the pulpability ...

    African Journals Online (AJOL)

    Eucalyptus globulus is one of the most important hardwood species used by the pulp and paper industry due to its high pulp yield, high wood density, excellent fibre quality and good handsheet properties. However, the wood is a highly variable and complex material that has different chemical, physical and anatomical ...

  11. determination of lipophilic extractives in ionic liquid extracts

    African Journals Online (AJOL)

    dell

    Chem. 9: 63-69. Freire CSR, Pinto PCR, Santiago AS,. Silvestre AJD, Evtuquin DV and Neto. CP 2006a Comparative study of lipophilic extractives of hardwoods and corresponding ECF bleached kraft pulps. BioResources. 1: 3-17. Freire CSR, Silvestre AJD and Neto CP. 2005. Lipophilic extractives in. Eucalyptus globulus.

  12. Analgesic and anti-inflammatory activities of ethanolic extract of ...

    African Journals Online (AJOL)

    The analgesic and anti-inflammatory activities of the ethanolic extract of Rheumatic Tea Formula (RTF) a polyherbal tea consisting the leaves of Eucalyptus globulus, Albizia chevalieri and bark of Salix alba were studied in mice and rats using acetic acid induced writhing, hot plate method, formalin induced pain and ...

  13. Avaliação da madeira de Eucalyptus camaldulensis Dehnh e Eucalyptus urophylla S.T. Blake em ensaios de usinagem, visando à produção moveleira Evaluation of the woods of Eucalyptus camaldulensis Dehnh and Eucalyptus urophylla S.T. Blake During machining tests for use by the furniture industry

    Directory of Open Access Journals (Sweden)

    Maria Odete Alves de Souza

    2009-08-01

    Full Text Available Este estudo teve como objetivo avaliar a potencialidade de uso da madeira de clones de Eucalyptus urophylla S. T. Blake, de 6 e 8 anos, e Eucalyptus camaldulensis Dehnh, de 10 anos, no que tange aos processos de usinagem, visando ao seu uso na indústria de móveis. A madeira utilizada originou-se de plantios comerciais cultivados em sistema de consórcio agrossilvipastoril, proveniente da Votorantim Metais Zinco S/A, situada no Município de Vazante, no Estado de Minas Gerais. Utilizaram-se seis árvores por clone, totalizando 18 exemplares. Foram realizados os seguintes ensaios de usinagem: corte paralelo às fibras, corte transversal às fibras, fresagem, aplainamento, furação, furação para espiga, furação para cavilha e moldura. Os resultados mostraram-se satisfatórios, com destaque para o clone de Eucalyptus urophylla com 8 anos, principalmente nos ensaios de corte paralelo e furação para espiga, apresentando grande potencial de uso da espécie para produção de móveis. A madeira dos clones testados apresentou bom desempenho na realização dos ensaios de usinagem, no que se refere à trabalhabilidade, não havendo entraves na sua utilização como fonte de matéria-prima na indústria moveleira.The objective of this research was to conduct machining tests in woods of 6 and 8-year-old Eucalyptus urophylla and of a ten-year old Eucalyptus camaldulensis and to evaluate their possible use by the furniture industry. The wood samples were obtained from six trees of each clone, planted in a consortium with cattle and grain, owned by Votorantim Metals and Zinc. The plantation is located in the neighborhood of the city of Vazante, in the State of Minas Gerais. The following essays were conducted: cut by a table saw either parallel or perpendicular to the grain; shaping; planing; drilling; routing; mortising and drilling for dowels. All the samples yielded satisfactory results, mainly the eight year old Eucalyptus urophylla, during

  14. Fumigant toxicity of plant essential oils against Camptomyia corticalis (Diptera: Cecidomyiidae).

    Science.gov (United States)

    Kim, Jun-Ran; Haribalan, Perumalsamy; Son, Bong-Ki; Ahn, Young-Joon

    2012-08-01

    The toxicity of 98 plant essential oils against third instars of cecidomyiid gall midge Camptomyia corticalis (Loew) (Diptera: Cecidomyiidae) was examined using a vapor-phase mortality bioassay. Results were compared with that of a conventional insecticide dichlorvos. Based on 24-h LC50 values, all essential oils were less toxic than dichlorvos (LC50, 0.027 mg/cm3). The LC50 of caraway (Carum carvi L.) seed, armoise (Artemisia vulgaris L.), clary sage (Salvia sclarea L.), oregano (Origanum vulgare L.), lemongrass [Cymbopogon citratus (DC.) Stapf], niaouli (Melaleuca viridiflora Gaertner), spearmint (Mentha spicata L.), cassia especial (Cinnamomum cassia Nees ex Blume), Dalmatian sage (Salvia offcinalis L.), red thyme (Thymus vulgaris L.), bay [Pimenta racemosa (P. Mill.) J.W. Moore], garlic (Allium sativum L.), and pennyroyal (Mentha pulegium L.) oils is between 0.55 and 0.60 mg/cm3. The LC50 of cassia (C. cassia, pure and redistilled), white thyme (T. vulgaris), star anise (Illicium verum Hook.f.), peppermint (Mentha X piperita L.), wintergreen (Gaultheria procumbens L.), cinnamon (Cinnamomum zeylanicum Blume) bark, sweet marjoram (Origanum majorana L.), Roman chamomile [Chamaemelum nobile (L.) All.], eucalyptus (Eucalyptus globulus Labill.), rosemary (Rosmarinus officinalis L.),Virginian cedarwood (Juniperus virginiana L.), pimento berry [Pimenta dioica (L.) Merr.], summer savory (Satureja hortensis L.), lavender (Lavandula angustifolia Mill.), and coriander (Coriandrum sativum L.) oils is between 0.61 and 0.99 mg/cm3. All other essential oils tested exhibited low toxicity to the cecidomyiid larvae (LC50, >0.99 mg/cm3). Global efforts to reduce the level of highly toxic synthetic insecticides in the agricultural environment justify further studies on the active essential oils as potential larvicides for the control of C. corticalis populations as fumigants with contact action.

  15. Caracterização tecnológica, para produção de celulose, da nova geração de clones de Eucalyptus do Brasil Technological characterization of the new generation of Eucalyptus clones in Brazil for kraft pulp production

    Directory of Open Access Journals (Sweden)

    José Lívio Gomide

    2005-02-01

    Full Text Available Foram analisados clones de Eucalyptus de última geração, pertencentes às principais empresas brasileiras de celulose. Os estudos consistiram numa caracterização tecnológica detalhada da qualidade das madeiras utilizadas no processamento fabril das empresas. Foram realizadas análise químicas detalhadas das madeiras e cozimentos kraft, simulando-se uma das modernas técnica de digestor contínuo. Os resultados indicaram a alta qualidade dos clones de Eucalyptus atualmente plantados no Brasil para produção de celulose.Last generation of Eucalyptus clones pulped by the main Brazilian kraft pulp mills were used for this study. Detailed chemical analysis of the woods were carried out to establish the wood quality. Laboratory simulation of a modern continuous digester technique was used for pulp production. Results demonstrated the high quality of Eucalyptus clones wood that are being planted in Brazil for kraft pulp production.

  16. Genomic research in Eucalyptus.

    Science.gov (United States)

    Poke, Fiona S; Vaillancourt, René E; Potts, Brad M; Reid, James B

    2005-09-01

    Eucalyptus L'Hérit. is a genus comprised of more than 700 species that is of vital importance ecologically to Australia and to the forestry industry world-wide, being grown in plantations for the production of solid wood products as well as pulp for paper. With the sequencing of the genomes of Arabidopsis thaliana and Oryza sativa and the recent completion of the first tree genome sequence, Populus trichocarpa, attention has turned to the current status of genomic research in Eucalyptus. For several eucalypt species, large segregating families have been established, high-resolution genetic maps constructed and large EST databases generated. Collaborative efforts have been initiated for the integration of diverse genomic projects and will provide the framework for future research including exploiting the sequence of the entire eucalypt genome which is currently being sequenced. This review summarises the current position of genomic research in Eucalyptus and discusses the direction of future research.

  17. Sub-acute toxicity evaluation of ethanol extract of rheumatic tea ...

    African Journals Online (AJOL)

    Sub-acute toxicity profile of Rheumatic Tea Formula (RTF), a polyherbal tea consisting of Salix alba, Eucalyptus globulus and Albizia chevalieri was investigated in wistar rats of both sexes. Wistar rats were orally administered three different doses of ethanol extract of RTF for 28 days after which the effect on body weight, ...

  18. Assessment of wood density of seven clones of Eucalyptus grandis ...

    African Journals Online (AJOL)

    With the objective of evaluating the correlation of wood basic density with age in seven Eucalyptus grandis clones planted in Brazil, five trees in each clone were sampled at the ages of 0, 5, 1, 5, 2, 5, 3, 5, 4, 5 and 7, 5 years. The analysis of these samples showed that the intraclonal variation of the basic density (except for 0, ...

  19. Efficiency of phosphorus (32 P) uptake and use by eucalyptus seedlings and rice

    International Nuclear Information System (INIS)

    Luca, Edgar Fernando de; Boaretto, Antonio Enedi

    2002-01-01

    The knowledge on different plant abilities to take up soil phosphorus and its use for growth can be important to improve markedly the efficiency of phosphorus fertilization. Having this in mind, an experiment was carried out under greenhouse conditions to test the hypothesis that eucalyptus seedlings are more efficient than rice in absorbing phosphorus from low solubility sources applied to a Quartzamment soil, testing different efficiency concepts. The phosphorus sources Ca(H 2 32 PO 4 ).H 2 O, CaH 32 PO 4 .2H 2 O and Ca 3 ( 32 PO 4 ) 2 , synthesised in laboratory and identified by X-ray diffractometry and thermal differential analyses, were used as radioactive tracers. It was concluded that rice is more efficient in absorbing phosphorus from these low solubility sources, while eucalyptus presents a higher coefficient of biologic P utilisation. The 'difference' method (conventional) that in based on P recovery by plants, underestimated the absorption of this nutrient for both species in relation to the isotopic method. (author)

  20. Profitability of Eucalyptus growing in Busiro, Mpigi District, Uganda

    African Journals Online (AJOL)

    supply of woody products from the existing forest resources is decreasing. ... Key words: Household level investments; Eucalyptus; financial profitability. ... eucalyptus (kalitunsi) tree species to other forest tree ... financial analyses is an assumption that market prices eucalyptus woodlot is situated in ... not be of good quality.

  1. Microwave-Assisted Extraction of Essential Oil from Eucalyptus: Study of the Effects of Operating Conditions

    Directory of Open Access Journals (Sweden)

    A.A. Saoud

    2006-12-01

    Full Text Available Classical extraction of essential oil such as Soxhlet and steam distillation is still a formidable and time-solvent consuming. Microwave assisted process (MAP is used to accelerate the extraction process of target compounds. It can be used for the extraction of compounds from various plants and animal tissues, or the extraction of undesirable components from raw materials. The investigation of microwave extraction of eucalyptus (globules essential oil using ethanol as solvent was carried out. The influence of material (eucalyptus/solvent (ethanol ratio, required doses of microwave, and time of microwave exposure on extraction efficiency, was studied.

  2. Effect of eucalyptus ( Eucalyptus camaldulensis ) and maize (Zea ...

    African Journals Online (AJOL)

    However eucalyptus plantations produce large amounts of litter, which impact on groundnut has not been clearly elucidated yet. In order to investigate litter accumulation effect on growth, development, and groundnut root infection by arbuscular mycorrhizal fungi (AMF) and rhizobia, a greenhouse experiment was performed ...

  3. The Transcriptome and Terpene Profile of Eucalyptus grandis Reveals Mechanisms of Defense Against the Insect Pest, Leptocybe invasa.

    Science.gov (United States)

    Oates, Caryn N; Külheim, Carsten; Myburg, Alexander A; Slippers, Bernard; Naidoo, Sanushka

    2015-07-01

    Plants have evolved complex defenses that allow them to protect themselves against pests and pathogens. However, there is relatively little information regarding the Eucalyptus defensome. Leptocybe invasa is one of the most damaging pests in global Eucalyptus forestry, and essentially nothing is known regarding the molecular mechanisms governing the interaction between the pest and host. The aim of the study was to investigate changes in the transcriptional landscape and terpene profile of a resistant and susceptible Eucalyptus genotype in an effort to improve our understanding of this interaction. We used RNA-seqencing to investigate transcriptional changes following L. invasa oviposition. Expression levels were validated using real-time quantitative PCR. Terpene profiles were investigated using gas chromatography coupled to mass spectometry on uninfested and oviposited leaves. We found 698 and 1,115 significantly differentially expressed genes from the resistant and susceptible interactions, respectively. Gene Ontology enrichment and Mapman analyses identified putative defense mechanisms including cell wall reinforcement, protease inhibitors, cell cycle suppression and regulatory hormone signaling pathways. There were significant differences in the mono- and sesquiterpene profiles between genotypes and between control and infested material. A model of the interaction between Eucalyptus and L. invasa was proposed from the transcriptomic and chemical data. © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  4. In silico analysis of Eucalyptus thioredoxins

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    Aulus Estevão Barbosa

    2005-01-01

    Full Text Available The Eucalyptus Genome Sequencing Project (FORESTs, an initiative from the Brazilian ONSA consortium (Organization for Nucleotide Sequencing and Analysis, has achieved the sequencing of 123.889 EST clones from 18 different cDNA libraries. We have investigated the FORESTs data set to identify EST clusters potentially encoding thioredoxins (TRX. Two types of thioredoxin families described in plants, chloroplastic (TRXm/f/x/y and cytosolic (TRXh, have been found in the transcriptome. Putative typical TRXs have been identified in fifteen clusters, four m-type, seven h-type, two f-type, one cluster for each x/y-types and one putative homologue of the TDX gene from Arabidopsis thaliana. One cluster presents an atypical active site WCMPS, different from the conserved WCGPC present in the other 15 clusters, and corresponds to a subgroup of cytosolic thioredoxins. Except in specific libraries from callus, roots, seedlings and wood tissues, thioredoxin deduced ESTs are found in all remaining libraries. According to the calculated frequencies of ESTs, chloroplastic thioredoxins are preferentially present in green tissues such as leaves whilst cytoplasmic thioredoxins are more general but demonstrate elevated frequencies in seedlings and flower tissues. TRX frequency patterns in the Eucalyptus transcriptome seem to indicate a good coherence with data from Arabidopsis thaliana gene expression.

  5. LEVANTAMENTO DA ENTOMOFAUNA EM PLANTIOS DE Eucalyptus spp. POR MEIO DE ARMADILHA LUMINOSA EM SÃO FRANCISCO DE ASSIS - RS

    Directory of Open Access Journals (Sweden)

    Juliana Garlet

    2016-01-01

    Full Text Available The cultivation of eucalyptus has become an important economic activity in Brazil. However, with the increase of the plantation areas, some entomological problems tend to increase in the same proportion. The constant monitoring of the insects associated with homogeneous planting systems is very important in order to seek the development of programs for integrated pest management. Thus, the purpose of this study was to perform a survey on the insect populations associated with Eucalyptus spp., in São Francisco de Assis, RS. The collects were carried out by light traps from July 2008 to August 2009 in three-year-old stands with the species: Eucalyptus dunni, Eucalyptus grandis and Eucalyptus grandis x Eucalyptus urophylla . The insects collected were analyzed using the faunistic indices, frequency, abundance, diversity, and constancy. The correlation between the pest species identified and the meteorological variables for the period were also performed. We collected 3623 individuals belonging to eight orders (Blattodea, Coleoptera, Dermaptera, Hemiptera, Hymenoptera, Lepidoptera, Mantodea and Odonata. Three species and three genus of lepidoptera defoliators considered of economic importance were identified: Automeris illustris, Eupseudosoma sp., Sabulodes sp., Sarsina sp., Thyrinteina arnobia and Agrotis ipsilon as well as the borer Phoracantha semipunctata . Our results show that there are important pest species of Eucalyptus already established in the region therefore some constant monitoring is required so that these species do not cause damage to plantations.

  6. Qualidade da celulose kraft-antraquinona de Eucalyptus dunnii plantado em cinco espaçamentos em relação ao Eucalyptus grandis e Eucalyptus saligna.

    Directory of Open Access Journals (Sweden)

    Giovanni Willer Ferreira

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 Este trabalho analisou o efeito de cinco espaçamentos de Eucalyptus dunnii (3 m x 1 m; 3 m x 1,5 m; 3 m x 2 m; 3 m x 3 m; 3 m x 4 m, para produção de celulose kraft-antraquinona, comparados com Eucalyptus grandis e Eucalyptus saligna (ambos no espaçamento 3 m x 2 m. Avaliaram-se a densidade básica e composição química da madeira original, analisando-se extrativos em diclorometano, pentosanas, lignina, solubilidade em NaOH8% e cinzas. Os cavacos foram submetidos a cozimento kraft-antraquinona com álcali ativo variando de 18,5 a 21% para obter um número kappa 17± 1,5; sulfidez a 8%, antraquinona base madeira 0,05%, relação licor/madeira 4:1, tempo até temperatura máxima 60 minutos, tempo à temperatura máxima 45 minutos. A celulose marrom resultante do cozimento kraft-antraquinona foi caracterizada e analisada em seus aspectos físico-químicos (rendimentos, rejeitos, número kappa, viscosidade intrínseca, alvura e solubilidade em NaOH5%. Uma parte desta sofreu refinação em moinho Jökro ao nível de 35°SR e, juntamente com a polpa não-refinada, foram submetidas a testes físico-mecânicos e óticos (resistência à tração, alongamento, estouro, rasgo, volume específico, alvura, resistência ao ar Gurley e ascensão capilar Klemm. O maior consumo de álcali ativo (20,5% ocorreu em espaçamento 3 m x 4 m, proporcionando maior degradação da polpa. Todos os espaçamentos caracterizados apresentaram viabilidade para a produção de celulose kraft-antraquinona, podendo proporcionar redução nos custos de produção de celulose, devido aos menores consumos apresentados de álcali ativo e madeira quando comparados com Eucalyptus grandis e Eucalyptus saligna. Os valores de extrativos e cinzas mostraram-se elevados para Eucalyptus dunnii, potencializando possíveis impactos em termos de incrustações e fechamento de circuito no processo fabril. Diante dos resultados

  7. RESISTÊNCIA BIOLÓGICA DA MADEIRA TRATADA DE Eucalyptus grandis E Eucalyptus cloeziana A FUNGOS APODRECEDORES EM ENSAIOS DE LABORATÓRIO

    Directory of Open Access Journals (Sweden)

    Magnos Alan Vivian

    2015-01-01

    Full Text Available The present study aimed to evaluate the biological resistance of treated wood from Eucalyptus grandis and Eucalyptus cloeziana under the action of biodeteriorative organisms in laboratory testing. Thus, we used trees of Eucalyptus grandis and Eucalyptus cloeziana, both 16 years old, which was converted into planks and subjected to preservative treatment in an autoclave with chromate copper arsenate (CCA. Then, it was made the specimens for the conduct of accelerated decay test, as recommended by ASTM. From the results, it was observed for the fungus Trametes versicolor that the preservative treatment was effective in reducing the biological degradation of the wood of the two species, with reduced mass loss in 35.17 and 82.31% for wood Eucalyptus grandis and Eucalyptus cloeziana, respectively, as for the fungus Gloeophyllum trabeum mass loss was reduced by 6.79 and 96.65%, compared to the control. Based on the conditions of realization of the present study, it was observed that preservative treatment with CCA is effective in the increasing the biological resistance of the wood under the action of fungi Trametes versicolor and Gloeophyllum trabeum.

  8. Neuroprotective and Anti-Aging Potentials of Essential Oils from Aromatic and Medicinal Plants

    Directory of Open Access Journals (Sweden)

    Muhammad Ayaz

    2017-05-01

    Full Text Available The use of essential oils (EOs and their components is known since long in traditional medicine and aromatherapy for the management of various diseases, and is further increased in the recent times. The neuroprotective and anti-aging potentials of EOs and their possible mechanism of actions were evaluated by numerous researchers around the globe. Several clinically important EOs and their components from Nigella sativa, Acorus gramineus, Lavandula angustifolia, Eucalyptus globulus, Mentha piperita, Rosmarinus officinalis, Jasminum sambac, Piper nigrum and so many other plants are reported for neuroprotective effects. This review article was aimed to summarize the current finding on EOs tested against neurodegenerative disorders like Alzheimer disease (AD and dementia. The effects of EOs on pathological targets of AD and dementia including amyloid deposition (Aβ, neurofibrillary tangles (NFTs, cholinergic hypofunction, oxidative stress and glutamatergic abnormalities were focused. Furthermore, effects of EOs on other neurological disorders including anxiety, depression, cognitive hypofunction epilepsy and convulsions were also evaluated in detail. In conclusion, EOs were effective on several pathological targets and have improved cognitive performance in animal models and human subjects. Thus, EOs can be developed as multi-potent agents against neurological disorders with better efficacy, safety and cost effectiveness.

  9. Oligo-carrageenan kappa increases NADPH, ascorbate and glutathione syntheses and TRR/TRX activities enhancing photosynthesis, basal metabolism, and growth in Eucalyptus trees.

    Science.gov (United States)

    González, Alberto; Moenne, Fabiola; Gómez, Melissa; Sáez, Claudio A; Contreras, Rodrigo A; Moenne, Alejandra

    2014-01-01

    In order to analyze the effect of OC kappa in redox status, photosynthesis, basal metabolism and growth in Eucalyptus globulus, trees were treated with water (control), with OC kappa at 1 mg mL(-1), or treated with inhibitors of NAD(P)H, ascorbate (ASC), and glutathione (GSH) syntheses and thioredoxin reductase (TRR) activity, CHS-828, lycorine, buthionine sulfoximine (BSO), and auranofin, respectively, and with OC kappa, and cultivated for 4 months. Treatment with OC kappa induced an increase in NADPH, ASC, and GSH syntheses, TRR and thioredoxin (TRX) activities, photosynthesis, growth and activities of basal metabolism enzymes such as rubisco, glutamine synthetase (GlnS), adenosine 5'-phosphosulfate reductase (APR), involved in C, N, and S assimilation, respectively, Krebs cycle and purine/pyrimidine synthesis enzymes. Treatment with inhibitors and OC kappa showed that increases in ASC, GSH, and TRR/TRX enhanced NADPH synthesis, increases in NADPH and TRR/TRX enhanced ASC and GSH syntheses, and only the increase in NADPH enhanced TRR/TRX activities. In addition, the increase in NADPH, ASC, GSH, and TRR/TRX enhanced photosynthesis and growth. Moreover, the increase in NADPH, ASC and TRR/TRX enhanced activities of rubisco, Krebs cycle, and purine/pyrimidine synthesis enzymes, the increase in GSH, NADPH, and TRR/TRX enhanced APR activity, and the increase in NADPH and TRR/TRX enhanced GlnS activity. Thus, OC kappa increases NADPH, ASC, and GSH syntheses leading to a more reducing redox status, the increase in NADPH, ASC, GSH syntheses, and TRR/TRX activities are cross-talking events leading to activation of photosynthesis, basal metabolism, and growth in Eucalyptus trees.

  10. Oligo-carrageenan kappa increases NADPH, ascorbate and glutathione syntheses and TRR/TRX activities enhancing photosynthesis, basal metabolism, and growth in Eucalyptus trees

    Directory of Open Access Journals (Sweden)

    Alberto eGonzález

    2014-10-01

    Full Text Available In order to analyze the effect of OC kappa in redox status, photosynthesis, basal metabolism and growth in Eucalyptus globulus, trees were treated with water (control, with OC kappa at 1 mg mL-1, or treated with inhibitors of NAD(PH, ascorbate (ASC and glutathione (GSH syntheses and thioredoxin reductase (TRR activity, CHS-828, lycorine, buthionine sulfoximine (BSO and auranofin, respectively, and with OC kappa, and cultivated for 4 months. Treatment with OC kappa induced an increase in NADPH, ASC, and GSH syntheses, TRR and thioredoxin (TRX activities, photosynthesis, growth and activities of basal metabolism enzymes such as rubisco, glutamine synthetase (GlnS, adenosine 5´-phosphosulfate reductase (APR, involved in C, N and S assimilation, respectively, Krebs cycle and purine/pyrimidine synthesis enzymes. Treatment with inhibitors and OC kappa showed that increases in ASC, GSH and TRR/TRX enhanced NADPH synthesis, increases in NADPH and TRR/TRX enhanced ASC and GSH syntheses, and only the increase in NADPH enhanced TRR/TRX activities. In addition, the increase in NADPH, ASC, GSH and TRR/TRX enhanced photosynthesis and growth. Moreover, the increase in NADPH, ASC and TRR/TRX enhanced activities of rubisco, Krebs cycle and purine/pyrimidine synthesis enzymes, the increase in GSH, NADPH, and TRR/TRX enhanced APR activity, and the increase in NADPH and TRR/TRX enhanced GlnS activity. Thus, OC kappa increases NADPH, ASC and GSH syntheses leading to a more reducing redox status, the increase in NADPH, ASC, GSH syntheses and TRR/TRX activities are cross-talking events leading to activation of photosynthesis, basal metabolism and growth in Eucalyptus trees.

  11. Estudo de algumas propriedades mecânicas da madeira de um híbrido clonal de Eucalyptus urophylla X Eucalyptus grandis Study of some mechanical properties of wood in a clonal hybrid of Eucalyptus urophylla X Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Fabrício Gomes Gonçalves

    2009-06-01

    Full Text Available A procura por madeiras oriundas de reflorestamentos destinadas à serraria é uma realidade já há muitos anos, principalmente aquelas das espécies do gênero Eucalyptus. Visando buscar novas informações importantes para esse mercado, este trabalho objetivou determinar algumas propriedades mecânicas da madeira de um híbrido clonal de Eucalyptus urophylla x Eucalyptus grandis de duas idades e provenientes de talhadia simples e de reforma. Os resultados indicaram que a madeira desse híbrido apresenta boas características tecnológicas, destacando-se a segunda tora (a partir de 3 m com as melhores propriedades de flexão estática (Módulo de Elasticidade - MOE e Módulo de Ruptura - MOR e Compressão Axial das fibras. As árvores de maior idade (166 meses e que sofreram dois desbastes apresentaram as melhores propriedades de flexão estática e compressão axial.The search for wood originated from reforestations destined to sawmill has been observed for many years, mainly for the Eucalyptus species. Searching for information to assist this market, the aim of this study was to determine some mechanical properties of the wood from a Eucalyptus urophylla x Eucalyptus grandis clonal hybrid, at two different ages and coming from coppice and reform. The results show that the wood of this clonal hybrid presents good technological characteristics and is the second best log (starting from 3,0 m, as to static bending properties (Module of Elasticity - MOE and Module of Rupture - MOR. The oldest trees (166 months - E2 which suffered two thinning presented the best results for the property of static bending and axial crushing (CA.

  12. Escala diagramática para quantificação da severidade de manchas em folhas de Eucalyptus globulus Labill. causadas por Teratosphaeria nubilosa (Cooke Crous & U. Braun

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    Martha Maria Passador

    2013-01-01

    Full Text Available The leaf spot (Mycosphaerella leaf disease = MLD caused by Teratosphaeria nubilosa has caused damage in eucalypt plantations in southern and southeastern Brazil. The need to assess the disease in the field to evaluate of this damage, efficiency control, evaluation of germplasm induces to the necessity of having a visual scale for evaluation of disease. The objective was to develop a diagrammatic scale for young leaves and one for adult leaves of Eucalyptus globules for MLD. To do so, the leaves collected in the field were scanned for image analysis. The damaged area, the healthy leaf area and the external area of the same scale RGB (Red, Green, Blue were determined. Subsequently, it was determinate the levels of severity depending on the sample distribution with seven levels for young leaves and six for adult leaves. For the visual acuity test and validate the scale, the leaves were evaluated for severity, with and without scale. With this proposed scales, the assessors showed good accuracy both for young and adult leaves with R2=0,98 and R2=0,80, respectively. The importance of the development of diagrammatic scales for assessing MLD in eucalyptus must to the fact that allows quantification of the symptoms accurately and precisely.

  13. Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis dehn and histological study of organogenesis in vitro

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    Roberson Dibax

    2010-04-01

    Full Text Available The present work aimed at regenerating plants of Eucalyptus camaldulensis from the cotyledonary explants and describing the anatomy of the tissues during callogenesis and organogenesis processes, in order to determine the origin of the buds. The cotyledonary leaves of E. camaldulensis were cultured in Murashige and Skoog (MS, WPM and JADS media supplemented with 2.7 µM NAA and 4.44 µM BAP. The best results for bud regeneration were obtained on MS and WPM media (57.5 and 55% of calluses formed buds, respectively. Shoot elongation and rooting (80% were obtained on MS/2 medium (with half-strength salt concentration with 0.2% activated charcoal. Acclimatization was performed in the growth chamber for 48 h and then the plants were transferred to a soil:vermiculite mixture and cultured in a greenhouse. Histological studies revealed that the callogenesis initiated in palisade parenchyma cells and that the adventitious buds were formed from the calluses, indicating indirect organogenesis.Este trabalho teve como objetivo a obtenção de plantas de Eucalyptus camaldulensis a partir de folhas cotiledonares e o estudo da anatomia dos tecidos durante a calogênese e organogênese para determinar a origem das gemas. Folhas cotiledonares foram cultivadas em meios de cultura MS, WPM e JADS suplementados com 2,7 µM de ANA e 4,44 µM de BAP. Os melhores resultados para a regeneração de gemas foram obtidos com os meios MS e WPM. Para o alongamento e enraizamento, o meio de cultura MS/2 contendo 0,2% de carvão ativado apresentou-se eficiente para ambas as etapas. A aclimatização foi realizada mediante a abertura dos frascos na sala de crescimento por 48 horas, seguido da transferência para casa-de-vegetação com nebulização intermitente. Estudos histológicos foram conduzidos e revelaram que a calogênese teve início nas células do parênquima paliçádico e que as gemas adventícias formaram-se a partir dos calos, indicando a organogênese indireta.

  14. Management of Fusarium oxysporum f. sp. capsici by leaf extract of Eucalyptus citriodora

    International Nuclear Information System (INIS)

    Shafique, S.; Asif, M.; Shafique, S.

    2015-01-01

    Fusarium wilt of chili (Capsicum annum L.) is an important disease in Pakistan that causes significant yield losses. In the present study, pathogenicity test was conducted using four strains of Fusarium oxysporum f.sp. capsici and ten chili varieties. It revealed that strain B was the most pathogenic strain and variety sky red was the most susceptible while variety Anchal was the most resistant against F. oxysporum strain B. Antifungal bioassays were conducted to find out antimycotic effect of extracts of fruit, bark and leaves of Eucalyptus citriodora (Hook.) against F. oxysporum. Ten concentrations (0, 1.0, 1.5, 2.0, 2.5 and 5%) of methanolic extracts of each plant part were employed against the target pathogen. Leaf extract imparted the maximum (up to 98%) and significant suppression in fungal growth while fruit and bark extracts proved less effective exhibiting only 50-60% reduction in fungal mycelial growth. The work concludes that methanolic extract of leaves of E. citriodora have potential to restrain the disastrous effects of the pathogenic fungus as the plant extracts of Eucalyptus conferred about 85% disease control in chilli plants with significantly high intensity of defense related enzymes under pathogenic stress. (author)

  15. The Eucalyptus grandis R2R3-MYB transcription factor family: evidence for woody growth-related evolution and function.

    Science.gov (United States)

    Soler, Marçal; Camargo, Eduardo Leal Oliveira; Carocha, Victor; Cassan-Wang, Hua; San Clemente, Hélène; Savelli, Bruno; Hefer, Charles A; Paiva, Jorge A Pinto; Myburg, Alexander A; Grima-Pettenati, Jacqueline

    2015-06-01

    The R2R3-MYB family, one of the largest transcription factor families in higher plants, controls a wide variety of plant-specific processes including, notably, phenylpropanoid metabolism and secondary cell wall formation. We performed a genome-wide analysis of this superfamily in Eucalyptus, one of the most planted hardwood trees world-wide. A total of 141 predicted R2R3-MYB sequences identified in the Eucalyptus grandis genome sequence were subjected to comparative phylogenetic analyses with Arabidopsis thaliana, Oryza sativa, Populus trichocarpa and Vitis vinifera. We analysed features such as gene structure, conserved motifs and genome location. Transcript abundance patterns were assessed by RNAseq and validated by high-throughput quantitative PCR. We found some R2R3-MYB subgroups with expanded membership in E. grandis, V. vinifera and P. trichocarpa, and others preferentially found in woody species, suggesting diversification of specific functions in woody plants. By contrast, subgroups containing key genes regulating lignin biosynthesis and secondary cell wall formation are more conserved across all of the species analysed. In Eucalyptus, R2R3-MYB tandem gene duplications seem to disproportionately affect woody-preferential and woody-expanded subgroups. Interestingly, some of the genes belonging to woody-preferential subgroups show higher expression in the cambial region, suggesting a putative role in the regulation of secondary growth. © 2014 The Authors New Phytologist © 2014 New Phytologist Trust.

  16. Eucalyptus Forest Information System for the Portuguese pulp and paper industry

    Science.gov (United States)

    Luis Fonseca; Rita Crespo; Henk Feith; Jose Luis Carvalho; Antonio Macedo; Joao Pedro Pina

    2000-01-01

    To support the management of the Portuguese eucalyptus forest, the Association of Portuguese Pulp and Paper Industries (CELPA) decided to develop a Eucalyptus Forest Information System (EFIS). The specific goals of the EFIS are: characterization and development of the eucalyptus forest over time; planning of successive national eucalyptus forest inventories; estimation...

  17. Chemical variation in a dominant tree species: population divergence, selection and genetic stability across environments.

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    Julianne M O'Reilly-Wapstra

    Full Text Available Understanding among and within population genetic variation of ecologically important plant traits provides insight into the potential evolutionary processes affecting those traits. The strength and consistency of selection driving variability in traits would be affected by plasticity in differences among genotypes across environments (G×E. We investigated population divergence, selection and environmental plasticity of foliar plant secondary metabolites (PSMs in a dominant tree species, Eucalyptus globulus. Using two common garden trials we examined variation in PSMs at multiple genetic scales; among 12 populations covering the full geographic range of the species and among up to 60 families within populations. Significant genetic variation in the expression of many PSMs resides both among and within populations of E. globulus with moderate (e.g., sideroxylonal A h(2op = 0.24 to high (e.g., macrocarpal G h(2op = 0.48 narrow sense heritabilities and high coefficients of additive genetic variation estimated for some compounds. A comparison of Qst and Fst estimates suggest that variability in some of these traits may be due to selection. Importantly, there was no genetic by environment interaction in the expression of any of the quantitative chemical traits despite often significant site effects. These results provide evidence that natural selection has contributed to population divergence in PSMs in E. globulus, and identifies the formylated phloroglucinol compounds (particularly sideroxylonal and a dominant oil, 1,8-cineole, as candidates for traits whose genetic architecture has been shaped by divergent selection. Additionally, as the genetic differences in these PSMs that influence community phenotypes is stable across environments, the role of plant genotype in structuring communities is strengthened and these genotypic differences may be relatively stable under global environmental changes.

  18. High-resolution genetic maps of Eucalyptus improve Eucalyptus grandis genome assembly.

    Science.gov (United States)

    Bartholomé, Jérôme; Mandrou, Eric; Mabiala, André; Jenkins, Jerry; Nabihoudine, Ibouniyamine; Klopp, Christophe; Schmutz, Jeremy; Plomion, Christophe; Gion, Jean-Marc

    2015-06-01

    Genetic maps are key tools in genetic research as they constitute the framework for many applications, such as quantitative trait locus analysis, and support the assembly of genome sequences. The resequencing of the two parents of a cross between Eucalyptus urophylla and Eucalyptus grandis was used to design a single nucleotide polymorphism (SNP) array of 6000 markers evenly distributed along the E. grandis genome. The genotyping of 1025 offspring enabled the construction of two high-resolution genetic maps containing 1832 and 1773 markers with an average marker interval of 0.45 and 0.5 cM for E. grandis and E. urophylla, respectively. The comparison between genetic maps and the reference genome highlighted 85% of collinear regions. A total of 43 noncollinear regions and 13 nonsynthetic regions were detected and corrected in the new genome assembly. This improved version contains 4943 scaffolds totalling 691.3 Mb of which 88.6% were captured by the 11 chromosomes. The mapping data were also used to investigate the effect of population size and number of markers on linkage mapping accuracy. This study provides the most reliable linkage maps for Eucalyptus and version 2.0 of the E. grandis genome. © 2014 CIRAD. New Phytologist © 2014 New Phytologist Trust.

  19. THE Eucalyptus sp. AGE PLANTATIONS INFLUENCING THE CARBON STOCKS

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    Charlote Wink

    2013-06-01

    Full Text Available http://dx.doi.org/10.5902/198050989279The tree growth and biomass accumulation, as well as the maintenance of forest residue at the soil surface can act in the removal of carbon from the atmosphere through the cycling process of plant material. The objective was to study the influence of Eucalyptus sp. Plantations with 20, 44 and 240 months of age on the variation of carbon in soil and biomass. The carbon in the soil depth was determined by CHNS auto-analyzer and carbon in the vegetation was determined by the biomass in each forest, considering a factor of 0.45 of the dry mass. We determined the density and particle size distribution of soil. For the comparison between plantations, there was analysis of variance and comparison of means of carbon in vegetation and soil, considering the 5% level of probability. The carbon content and stock in the soil were low, indicating that a natural feature of the category of Paleuldt, or the growth of eucalyptus forests, replacing the field native vegetation did not aggregate a significant increase in the carbon. Although, there was a significant increase carbon in aboveground biomass. It includes forest biomass and litter. So, despite the values ​​of carbon stocks are low, it identified a greater average total in the soil compared to the stock aboveground. Furthermore, this increase aboveground (tree and litter compartments can be considered significant between the eucalyptus plantations of different ages.

  20. The tonoplast intrinsic aquaporin (TIP) subfamily of Eucalyptus grandis: Characterization of EgTIP2, a root-specific and osmotic stress-responsive gene.

    Science.gov (United States)

    Rodrigues, Marcela I; Bravo, Juliana P; Sassaki, Flávio T; Severino, Fábio E; Maia, Ivan G

    2013-12-01

    Aquaporins have important roles in various physiological processes in plants, including growth, development and adaptation to stress. In this study, a gene encoding a root-specific tonoplast intrinsic aquaporin (TIP) from Eucalyptus grandis (named EgTIP2) was investigated. The root-specific expression of EgTIP2 was validated over a panel of five eucalyptus organ/tissues. In eucalyptus roots, EgTIP2 expression was significantly induced by osmotic stress imposed by PEG treatment. Histochemical analysis of transgenic tobacco lines (Nicotiana tabacum SR1) harboring an EgTIP2 promoter:GUS reporter cassette revealed major GUS staining in the vasculature and in root tips. Consistent with its osmotic-stress inducible expression in eucalyptus, EgTIP2 promoter activity was up-regulated by mannitol treatment, but was down-regulated by abscisic acid. Taken together, these results suggest that EgTIP2 might be involved in eucalyptus response to drought. Additional searches in the eucalyptus genome revealed the presence of four additional putative TIP coding genes, which could be individually assigned to the classical TIP1-5 groups. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  1. Insect pests of Eucalyptus and their control

    Energy Technology Data Exchange (ETDEWEB)

    Sen-Sarma, P K; Thakur, M L

    1983-12-01

    In India, about sixty odd species of insects have so far been recorded to be associated with Eucalyptus. Important pests are some xylophagous insects, sap suckers, defoliators and termites. Of these, stem and root borer, Celostrna scabrator Fabr, and some species of termites have been recognised as key pests, whereas Apogonia coriaces Waterhouse, Mimeta mundissima Walker (Coleoptera: Scarabaeidae), Agrotis ipsilon Hufnagel (Lepidoptera: Noctuidae), Brachytrypus portenosus Lichtenstein and Gymmogryllus humeralis Walker (Orthoptera: Gryllidae) are likely to become potential pests in Eucalyptus nurseries. In this paper available information on insect pests of Eucalyptus, their bioecology and control measures have been presented.

  2. Transcriptional profiles of hybrid Eucalyptus genotypes with contrasting lignin content reveal that monolignol biosynthesis-related genes regulate wood composition

    Directory of Open Access Journals (Sweden)

    Tomotaka eShinya

    2016-04-01

    Full Text Available Eucalyptus species constitutes the most widely planted hardwood trees in temperate and subtropical regions. In this study, we compared the transcript levels of genes involved in lignocellulose formation such as cellulose, hemicellulose and lignin biosynthesis in two selected three-year old hybrid Eucalyptus (Eucalyptus urophylla x E. grandis genotypes (AM063 and AM380 that have different lignin content. AM063 and AM380 had 20.2 and 35.5% of Klason lignin content and 59.0% and 48.2%, -cellulose contents, respectively. We investigated the correlation between wood properties and transcript levels of wood formation-related genes using RNA-seq with total RNAs extracted from developing xylem tissues at a breast height. Transcript levels of cell wall construction genes such as cellulose synthase (CesA and sucrose synthase (SUSY were almost the same in both genotypes. However, AM063 exhibited higher transcript levels of UDP-glucose pyrophosphorylase (UGP and xyloglucan endotransglucoxylase (XTH than those in AM380. Most monolignol biosynthesis- related isozyme genes showed higher transcript levels in AM380. These results indicate monolignol biosynthesis-related genes may regulate wood composition in Eucalyptus. Flavonoids contents were also observed at much higher levels in AM380 as a result of the elevated transcript levels of common phenylpropanoid pathway genes, phenylalanine ammonium lyase (PAL, cinnamate-4-hydroxylase (C4H and 4-coumarate-CoA ligase (4CL. Secondary plant cell wall formation is regulated by many transcription factors. We analyzed genes encoding NAC, WRKY, AP2/ERF and KNOX transcription factors and found higher transcript levels of these genes in AM380. We also observed increased transcription of some MYB and LIM domain transcription factors in AM380 compared to AM063. All these results show that genes related to monolignol biosynthesis may regulate the wood composition and help maintain the ratio of cellulose and lignin contents

  3. Intercropping Acacia mangium stimulates AMF colonization and soil phosphatase activity in Eucalyptus grandis

    OpenAIRE

    Bini, Daniel; Santos, Cristiane Alcantara dos; Silva, Mylenne Calcciolari Pinheiro da; Bonfim, Joice Andrade; Cardoso, Elke Jurandy Bran Nogueira

    2018-01-01

    ABSTRACT: Arbuscular mycorrhizal fungi (AMF) are very important to plant nutrition, mostly in terms of acquisition of P and micronutrients. While Acacia mangium is closely associated with AMF throughout the whole cycle, Eucalyptus grandis presents this symbiosis primarily at the seedling stage. The aim of this study was to evaluate the dynamics of AMF in these two tree species in both pure and mixed plantations during the first 20 months after planting. We evaluated the abundance, richness an...

  4. USE OF EUCALYPTUS WOOD FOR ORIENTED STRAND BOARD (OSB MANUFACTURING

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    Setsuo Iwakiri

    2004-06-01

    Full Text Available This research evaluated the potential use of Eucalyptus species for OSB manufacturing. The boards were manufactured at the density of 0,70 g/cm³ and 6% of the phenol-formaldheyde resin contents. The following Eucalyptus species were studied: E. grandis E. dunnii ,E. tereticornis E. saligna ,E. citriodora, and E. maculata. The results of the physical and mechanical property tests showed high potentiality of the uses of Eucalyptus grandis and Eucalyptus saligna for OSB manufacturing, Boards manufactured with Eucalyptus grandis wood presented similar or higher average values for physical and mechanical properties, in comparison to Pinus taeda, which is the main species used for OSB production in Brazil.

  5. Genes encoding enzymes of the lignin biosynthesis pathway in Eucalyptus

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    Ricardo Harakava

    2005-01-01

    Full Text Available Eucalyptus ESTs libraries were screened for genes involved in lignin biosynthesis. This search was performed under the perspective of recent revisions on the monolignols biosynthetic pathway. Eucalyptus orthologues of all genes of the phenylpropanoid pathway leading to lignin biosynthesis reported in other plant species were identified. A library made with mRNAs extracted from wood was enriched for genes involved in lignin biosynthesis and allowed to infer the isoforms of each gene family that play a major role in wood lignin formation. Analysis of the wood library suggests that, besides the enzymes of the phenylpropanoids pathway, chitinases, laccases, and dirigent proteins are also important for lignification. Colocalization of several enzymes on the endoplasmic reticulum membrane, as predicted by amino acid sequence analysis, supports the existence of metabolic channeling in the phenylpropanoid pathway. This study establishes a framework for future investigations on gene expression level, protein expression and enzymatic assays, sequence polymorphisms, and genetic engineering.

  6. Optimal management and productivity of Eucalyptus grandis on former phosphate mined and citrus lands in central and southern Florida: influence of genetics and spacing

    Science.gov (United States)

    Kyle W. Fabbro; Donald L. Rockwood

    2016-01-01

    Eucalyptus short rotation woody crops (SRWC) with superior genotypes are promising in central and south Florida due to their fast growth, freeze resilience, coppicing ability, and site tolerance. Four Eucalyptus grandis cultivars, E.nergy™ G1, G2, G3, and/or G5, were established in 2009 at varying planting densities on a...

  7. Classification of Small-Scale Eucalyptus Plantations Based on NDVI Time Series Obtained from Multiple High-Resolution Datasets

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    Hailang Qiao

    2016-02-01

    Full Text Available Eucalyptus, a short-rotation plantation, has been expanding rapidly in southeast China in recent years owing to its short growth cycle and high yield of wood. Effective identification of eucalyptus, therefore, is important for monitoring land use changes and investigating environmental quality. For this article, we used remote sensing images over 15 years (one per year with a 30-m spatial resolution, including Landsat 5 thematic mapper images, Landsat 7-enhanced thematic mapper images, and HJ 1A/1B images. These data were used to construct a 15-year Normalized Difference Vegetation Index (NDVI time series for several cities in Guangdong Province, China. Eucalyptus reference NDVI time series sub-sequences were acquired, including one-year-long and two-year-long growing periods, using invested eucalyptus samples in the study region. In order to compensate for the discontinuity of the NDVI time series that is a consequence of the relatively coarse temporal resolution, we developed an inverted triangle area methodology. Using this methodology, the images were classified on the basis of the matching degree of the NDVI time series and two reference NDVI time series sub-sequences during the growing period of the eucalyptus rotations. Three additional methodologies (Bounding Envelope, City Block, and Standardized Euclidian Distance were also tested and used as a comparison group. Threshold coefficients for the algorithms were adjusted using commission–omission error criteria. The results show that the triangle area methodology out-performed the other methodologies in classifying eucalyptus plantations. Threshold coefficients and an optimal discriminant function were determined using a mosaic photograph that had been taken by an unmanned aerial vehicle platform. Good stability was found as we performed further validation using multiple-year data from the high-resolution Gaofen Satellite 1 (GF-1 observations of larger regions. Eucalyptus planting dates

  8. Propriedades de chapas de flocos fabricadas com adesivo de uréia-formaldeído e de taninos da casca de Eucalyptus grandis W. Hill ex Maiden ou de Eucalyptus pellita F. Muell. Properties of flakeboards made from urea-formaldehyde and bark tannins adhesives of Eucalyptus grandis or Eucalyptus pellita

    Directory of Open Access Journals (Sweden)

    Angélica de Cássia Oliveira Carneiro

    2004-10-01

    Full Text Available Os taninos foram extraídos da casca de Eucalyptus grandis e Eucalyptus pellita, com água quente, à qual se adicionaram 4,5% de sulfito de sódio, durante três horas. As temperaturas da solução foram iguais a 70 e 100 ºC para Eucalyptus grandis e Eucalyptus pellita, respectivamente. Para a produção dos adesivos e com o intuito de reduzir a sua viscosidade, os taninos foram sulfitados com sulfito de sódio e ácido acético. Formulações adesivas foram preparadas adicionando-se 0, 25, 50, 75 ou 100% de adesivos tânicos ao adesivo comercial de uréia-formaldeído. Foram fabricadas chapas de flocos de Pinus elliottii Engelm. e Eucalyptus grandis, utilizando-se 8% da formulação adesiva. As propriedades das chapas foram determinadas segundo a norma ASTM D-1037, de 1993. Observou-se que as propriedades das chapas foram superiores ao mínimo estabelecido pela norma ANSI/A 280.1-93, exceto no caso da resistência à umidade. Verificou-se, ainda, que o emprego de uma formulação adesiva contendo resina à base de uréia-formaldeído e tanino-formaldeído pode melhorar algumas propriedades.Bark tannins of Eucalyptus grandis and Eucalyptus pellita were extracted with 4,5% sodium sulfite in hot water solution for a period of three hours. Solution temperatures were 70 and 100ºC, for Eucalyptus grandis and Eucalyptus pellita bark respectively. Tannins were reacted with acetic acid and sodium sulfite to reduce adhesive viscosity. Adhesive formulations were prepared adding 0, 25, 50, 75 or 100% of tannin adhesives to the commercial urea-formaldehyde adhesive. Flakeboards were fabricated with 8% resin content. Board properties were determined according to ASTM D-1037 standards. Except for humidity, all board properties were superior to the values established by ANSI.A 208.1-93 commercial standard. Addition of tannins to the urea-formaldehyde adhesive improved some properties.

  9. Genomic Characterization of DArT Markers Based on High-Density Linkage Analysis and Physical Mapping to the Eucalyptus Genome

    Science.gov (United States)

    Petroli, César D.; Sansaloni, Carolina P.; Carling, Jason; Steane, Dorothy A.; Vaillancourt, René E.; Myburg, Alexander A.; da Silva, Orzenil Bonfim; Pappas, Georgios Joannis; Kilian, Andrzej; Grattapaglia, Dario

    2012-01-01

    Diversity Arrays Technology (DArT) provides a robust, high throughput, cost-effective method to query thousands of sequence polymorphisms in a single assay. Despite the extensive use of this genotyping platform for numerous plant species, little is known regarding the sequence attributes and genome-wide distribution of DArT markers. We investigated the genomic properties of the 7,680 DArT marker probes of a Eucalyptus array, by sequencing them, constructing a high density linkage map and carrying out detailed physical mapping analyses to the Eucalyptus grandis reference genome. A consensus linkage map with 2,274 DArT markers anchored to 210 microsatellites and a framework map, with improved support for ordering, displayed extensive collinearity with the genome sequence. Only 1.4 Mbp of the 75 Mbp of still unplaced scaffold sequence was captured by 45 linkage mapped but physically unaligned markers to the 11 main Eucalyptus pseudochromosomes, providing compelling evidence for the quality and completeness of the current Eucalyptus genome assembly. A highly significant correspondence was found between the locations of DArT markers and predicted gene models, while most of the 89 DArT probes unaligned to the genome correspond to sequences likely absent in E. grandis, consistent with the pan-genomic feature of this multi-Eucalyptus species DArT array. These comprehensive linkage-to-physical mapping analyses provide novel data regarding the genomic attributes of DArT markers in plant genomes in general and for Eucalyptus in particular. DArT markers preferentially target the gene space and display a largely homogeneous distribution across the genome, thereby providing superb coverage for mapping and genome-wide applications in breeding and diversity studies. Data reported on these ubiquitous properties of DArT markers will be particularly valuable to researchers working on less-studied crop species who already count on DArT genotyping arrays but for which no reference

  10. Effects of Successive Rotation Regimes on Carbon Stocks in Eucalyptus Plantations in Subtropical China Measured over a Full Rotation.

    Science.gov (United States)

    Li, Xiaoqiong; Ye, Duo; Liang, Hongwen; Zhu, Hongguang; Qin, Lin; Zhu, Yuling; Wen, Yuanguang

    2015-01-01

    Plantations play an important role in carbon sequestration and the global carbon cycle. However, there is a dilemma in that most plantations are managed on short rotations, and the carbon sequestration capacities of these short-rotation plantations remain understudied. Eucalyptus has been widely planted in the tropics and subtropics due to its rapid growth, high adaptability, and large economic return. Eucalyptus plantations are primarily planted in successive rotations with a short rotation length of 6~8 years. In order to estimate the carbon-stock potential of eucalyptus plantations over successive rotations, we chose a first rotation (FR) and a second rotation (SR) stand and monitored the carbon stock dynamics over a full rotation from 1998 to 2005. Our results showed that carbon stock in eucalyptus trees (TC) did not significantly differ between rotations, while understory vegetation (UC) and soil organic matter (SOC) stored less carbon in the SR (1.01 vs. 2.76 Mg.ha(-1) and 70.68 vs. 81.08 Mg. ha(-1), respectively) and forest floor carbon (FFC) conversely stored more (2.80 vs. 2.34 Mg. ha(-1)). The lower UC and SOC stocks in the SR stand resulted in 1.13 times lower overall ecosystem carbon stock. Mineral soils and overstory trees were the two dominant carbon pools in eucalyptus plantations, accounting for 73.77%~75.06% and 20.50%~22.39%, respectively, of the ecosystem carbon pool. However, the relative contribution (to the ecosystem pool) of FFC stocks increased 1.38 times and that of UC decreased 2.30 times in the SR versus FR stand. These carbon pool changes over successive rotations were attributed to intensive successive rotation regimes of eucalyptus plantations. Our eight year study suggests that for the sustainable development of short-rotation plantations, a sound silvicultural strategy is required to achieve the best combination of high wood yield and carbon stock potential.

  11. Effects of Successive Rotation Regimes on Carbon Stocks in Eucalyptus Plantations in Subtropical China Measured over a Full Rotation.

    Directory of Open Access Journals (Sweden)

    Xiaoqiong Li

    Full Text Available Plantations play an important role in carbon sequestration and the global carbon cycle. However, there is a dilemma in that most plantations are managed on short rotations, and the carbon sequestration capacities of these short-rotation plantations remain understudied. Eucalyptus has been widely planted in the tropics and subtropics due to its rapid growth, high adaptability, and large economic return. Eucalyptus plantations are primarily planted in successive rotations with a short rotation length of 6~8 years. In order to estimate the carbon-stock potential of eucalyptus plantations over successive rotations, we chose a first rotation (FR and a second rotation (SR stand and monitored the carbon stock dynamics over a full rotation from 1998 to 2005. Our results showed that carbon stock in eucalyptus trees (TC did not significantly differ between rotations, while understory vegetation (UC and soil organic matter (SOC stored less carbon in the SR (1.01 vs. 2.76 Mg.ha(-1 and 70.68 vs. 81.08 Mg. ha(-1, respectively and forest floor carbon (FFC conversely stored more (2.80 vs. 2.34 Mg. ha(-1. The lower UC and SOC stocks in the SR stand resulted in 1.13 times lower overall ecosystem carbon stock. Mineral soils and overstory trees were the two dominant carbon pools in eucalyptus plantations, accounting for 73.77%~75.06% and 20.50%~22.39%, respectively, of the ecosystem carbon pool. However, the relative contribution (to the ecosystem pool of FFC stocks increased 1.38 times and that of UC decreased 2.30 times in the SR versus FR stand. These carbon pool changes over successive rotations were attributed to intensive successive rotation regimes of eucalyptus plantations. Our eight year study suggests that for the sustainable development of short-rotation plantations, a sound silvicultural strategy is required to achieve the best combination of high wood yield and carbon stock potential.

  12. Genomic characterization of DArT markers based on high-density linkage analysis and physical mapping to the Eucalyptus genome.

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    César D Petroli

    Full Text Available Diversity Arrays Technology (DArT provides a robust, high throughput, cost-effective method to query thousands of sequence polymorphisms in a single assay. Despite the extensive use of this genotyping platform for numerous plant species, little is known regarding the sequence attributes and genome-wide distribution of DArT markers. We investigated the genomic properties of the 7,680 DArT marker probes of a Eucalyptus array, by sequencing them, constructing a high density linkage map and carrying out detailed physical mapping analyses to the Eucalyptus grandis reference genome. A consensus linkage map with 2,274 DArT markers anchored to 210 microsatellites and a framework map, with improved support for ordering, displayed extensive collinearity with the genome sequence. Only 1.4 Mbp of the 75 Mbp of still unplaced scaffold sequence was captured by 45 linkage mapped but physically unaligned markers to the 11 main Eucalyptus pseudochromosomes, providing compelling evidence for the quality and completeness of the current Eucalyptus genome assembly. A highly significant correspondence was found between the locations of DArT markers and predicted gene models, while most of the 89 DArT probes unaligned to the genome correspond to sequences likely absent in E. grandis, consistent with the pan-genomic feature of this multi-Eucalyptus species DArT array. These comprehensive linkage-to-physical mapping analyses provide novel data regarding the genomic attributes of DArT markers in plant genomes in general and for Eucalyptus in particular. DArT markers preferentially target the gene space and display a largely homogeneous distribution across the genome, thereby providing superb coverage for mapping and genome-wide applications in breeding and diversity studies. Data reported on these ubiquitous properties of DArT markers will be particularly valuable to researchers working on less-studied crop species who already count on DArT genotyping arrays but for

  13. Utilização de madeiras de Eucalyptus grandis E Eucalyptus dunnii para produção de painéis de partículas orientadas – OSB

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    Setsuo Iwakiri

    2008-01-01

    Full Text Available This study was developed to evaluate the feasibility of OSB manufacturing using woods of Eucalyptus grandis and Eucalyptus dunnii. Boards with nominal density of 0,70 g/cm³ and 1,0 g/cm³ were manufactured in laboratory, using 100% of wood particles from Pinus taeda, Eucalyptus grandis and Eucalyptus dunnii, and mixtures of 50% of Pinus taeda in the internal layer of the board, with 50% of Eucalyptus grandis and 50% of Eucalyptus dunnii. The boards of Eucalyptus grandis with density of 0,70 g/cm³, as standard board density, showed the values of properties compatible with the requirements of the Canadian and European Standards and also in relation of boards manufactured from Pinus taeda. The results of the mechanical properties showed an increase in the MOE and MOR in static bending with the increase in the board density, opening the possibility to use the high density OSB for applications requiring higher strength. The results of this research indicate that wood of Eucalyptus grandis can be used as alternative specie to OSB manufacturing in the Brazil.

  14. Development Potential, in a Greenhouse, of Cuttings of the Hybrid Eucalyptus grandis x Eucalyptus camaldulensis

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    M. N. Garcia

    2013-03-01

    Full Text Available Abstract: This study evaluated the potential for development in the greenhouse, cuttings of the hybrid of Eucalyptus grandis x Eucalyptus camaldulensis in Sinop - Mato Grosso and generated equations to estimate the percentage of rooting parameters. Were measured: the percentage of cuttings with some degree of modification, the percentage of cuttings in the percentage of cuttings with roots larger than 10 cm in length. Also measured was the neck diameter, height and leaf area index. The heights and leaf area showed lower development within the greenhouse. The rooting potential is based on curve speed of rooting and was found 12 days after staking. The identification of the optimal point of withdrawal of the shoots of the greenhouse through the intercept curves daily current increase curve (DCI e daily average increase (DAI, being 19 days after staking. The company derives the stakes with 24 days underdoing the potential of the species and structures used.Keywords: Eucalyptus, greenhouse management and rooting

  15. Population Dynamics of Lepidoptera Pests in Eucalyptus urophylla Plantations in the Brazilian Amazonia

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    José Cola Zanuncio

    2014-01-01

    Full Text Available Forestry companies study the population dynamics of insect pests in Integrated Pest Management for cost effectiveness. The objective of this study was to obtain qualitative and quantitative information on population fluctuation of the Lepidopteran defoliators of Eucalyptus urophylla plants in the Brazilian Amazon rainforest. In all, 402 species were collected, of which 10 were primary pests, nine were secondary pests, and the remaining bore no definite relevance to eucalyptus. Primary pests formed a low percentage of the total species, although they recorded a high percentage of the total number of individuals. The abundance of secondary pests, except in Caracuru, was less than 150 specimens annually. Primary pests showed higher population peaks during periods of low precipitation. The small number of species and the high abundance of primary and secondary pests could be due to the availability of food, or a deficiency in natural biological control. This suggests the possibilities of population outbreaks in the eucalyptus plantations. The period of highest occurrence for insect species in these crops must be identified so that suitable strategies can be developed for Integrated Pest Management.

  16. Environmental aspects of eucalyptus based ethanol production and use

    International Nuclear Information System (INIS)

    González-García, Sara; Moreira, Ma. Teresa; Feijoo, Gumersindo

    2012-01-01

    A renewable biofuel economy is projected as a pathway to decrease dependence on fossil fuels as well as to reduce greenhouse gases (GHG) emissions. Ethanol produced on large-scale from lignocellulosic materials is considered the automotive fuel with the highest potential. In this paper, a life cycle assessment (LCA) study was developed to evaluate the environmental implications of the production of ethanol from a fast-growing short rotation crop (SRC): eucalyptus as well as its use in a flexi-fuel vehicle (FFV). The aim of the analysis was to assess the environmental performance of three ethanol based formulations: E10, E85 and E100, in comparison with conventional gasoline. The standard framework of LCA from International Standards Organization was followed and the system boundaries included the cultivation of the eucalyptus biomass, the processing to ethanol conversion, the blending with gasoline (when required) and the final use of fuels. The environmental results show reductions in all impact categories under assessment when shifting to ethanol based fuels, excluding photochemical oxidant formation, eutrophication as well as terrestrial and marine ecotoxicity which were considerably influenced by upstream activities related to ethanol manufacture. The LCA study remarked those stages where the researchers and technicians need to work to improve the environmental performance. Special attention must be paid on ethanol production related activities, such as on-site energy generation and distillation, as well as forest activities oriented to the biomass production. The use of forest machinery with higher efficiency levels, reduction of fertilizers dose and the control of diffuse emissions from the conversion plant would improve the environmental profile. -- Highlights: ► The identification of the environmental implications of the production and use of eucalyptus based ethanol was carried out. ► Eucalyptus is a Spanish common and abundant fast-growing short

  17. Qualidade da celulose kraft-antraquinona de Eucalyptus dunnii plantado em cinco espaçamentos em relação ao Eucalyptus grandis e Eucalyptus saligna.

    OpenAIRE

    Giovanni Willer Ferreira; Jorge Vieira Gonzaga; Celso Edmundo B. Foelkel; Teotônio Francisco de Assis; Edvins Ratnieks; Maria Cladis Mezzomo da Silva

    2009-01-01

    Este trabalho analisou o efeito de cinco espaçamentos de Eucalyptus dunnii (3 m x 1 m; 3 m x 1,5 m; 3 m x 2 m; 3 m x 3 m; 3 m x 4 m), para produção de celulose kraft-antraquinona, comparados com Eucalyptus grandis e Eucalyptus saligna (ambos no espaçamento 3 m x 2 m). Avaliaram-se a densidade básica e composição química da madeira original, analisando-se extrativos em diclorometano, pentosanas, lignina, solubilidade em NaOH8% e cinzas. Os cavacos foram submetidos a cozimento kraft-antraqu...

  18. Genetic transformation of Eucalyptus camaldulensis by agrobalistic method

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    Evânia Galvão Mendonça

    2013-06-01

    Full Text Available Eucalyptus stands in the setting of worldwide forestry due to its adaptability, rapid growth, production of high-quality and low cost of wood pulp fibers. The eucalyptus convetional breeding is impaired mainlly by the long life cycle making the genetic transformation systems an important tool for this purpose. However, this system requires in vitro eficient protocols for plant induction, regeneration and seletion, that allow to obtain transgenic plants from the transformed cell groups. The aim of this work was to evaluate the callus formation and to optimize the leaves and callus genetic transformation protocol by using the Agrobacterium tumefaciens system. Concerning callus formation, two different culture media were evaluated: MS medium supplemented with auxin, cytokinin (M1 and the MS medium with reduced nitrogen concentration and supplemented with auxin, cytokinin coconut water (M2. To establish the leave genetic transformation, those were exposed to agrobiolistics technique (gene gun, to tissue injury, and A. tumesfasciens EHA 105 contening the vetor pCambia 3301 (35S::GUS::NOS, for gene transference and to establish the callus transformation thoses were exposed only to A. tumefasciens. For both experiments, the influence of different infection periods was evaluated. The M2 medium provided the best values for callus sizea and fresh and dry weight. The leaves genetic transformation using the agrobiolistics technique was effective, the gus gene transient expression could be observed. No significant differences were obtained in the infection periods (4, 6 and 8 minutes. The callus genetic transformation with A. tumefaciens also promotend the gus gene transient expression on the callus co-cultiveted for 15 e 30 minutes. The transformed callus was transfered to a regeneration and selection medium and transformed plants were obtained.

  19. Evaluating potential changes in fire risk from Eucalyptus plantings in the Southern United States

    Science.gov (United States)

    Scott L. Goodrick; John A. Stanturf

    2012-01-01

    Renewed interest in short-rotation woody crops for bioenergy and bioproducts has prompted a reevaluation of the Eucalyptus species for the southern United States. One question that arises about the potential effects of introducing a nonnative species is what effect will there be on fire behavior. Our approximate answer based on modeling fire behavior...

  20. High-throughput SNP genotyping in the highly heterozygous genome of Eucalyptus: assay success, polymorphism and transferability across species

    Science.gov (United States)

    2011-01-01

    Background High-throughput SNP genotyping has become an essential requirement for molecular breeding and population genomics studies in plant species. Large scale SNP developments have been reported for several mainstream crops. A growing interest now exists to expand the speed and resolution of genetic analysis to outbred species with highly heterozygous genomes. When nucleotide diversity is high, a refined diagnosis of the target SNP sequence context is needed to convert queried SNPs into high-quality genotypes using the Golden Gate Genotyping Technology (GGGT). This issue becomes exacerbated when attempting to transfer SNPs across species, a scarcely explored topic in plants, and likely to become significant for population genomics and inter specific breeding applications in less domesticated and less funded plant genera. Results We have successfully developed the first set of 768 SNPs assayed by the GGGT for the highly heterozygous genome of Eucalyptus from a mixed Sanger/454 database with 1,164,695 ESTs and the preliminary 4.5X draft genome sequence for E. grandis. A systematic assessment of in silico SNP filtering requirements showed that stringent constraints on the SNP surrounding sequences have a significant impact on SNP genotyping performance and polymorphism. SNP assay success was high for the 288 SNPs selected with more rigorous in silico constraints; 93% of them provided high quality genotype calls and 71% of them were polymorphic in a diverse panel of 96 individuals of five different species. SNP reliability was high across nine Eucalyptus species belonging to three sections within subgenus Symphomyrtus and still satisfactory across species of two additional subgenera, although polymorphism declined as phylogenetic distance increased. Conclusions This study indicates that the GGGT performs well both within and across species of Eucalyptus notwithstanding its nucleotide diversity ≥2%. The development of a much larger array of informative SNPs across

  1. TEORES DE NUTRIENTES EM POVOAMENTOS MONOESPECÍFICOS E MISTOS DE Eucalyptus urograndis e Acacia mearnsii em SISTEMA AGROSSILVICULTURAL

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    Márcio Viera

    2013-01-01

    Full Text Available The study had as objective compare the nutrients content in the different species involved in monospecific and mixed stands of Eucalyptus urograndis and Acacia mearnsii and in a consortium with Zea mays. The determination for forest species nutrients concentration, the treatments 100E (100 % eucalyptus + maize; 100A (100 % black-wattle + maize and 50E:50A (50 % eucalyptus + 50 % black-wattle + maize, and in the maize were done in treatments 100E; 100A, 50E:50A; 75E:25A (75 % eucalyptus + 25 % black-wattle + maize and 25E:75A (25 % eucalyptus + 75 % black-wattle + maize. The experimental design was a randomized block design with three replications. Forests species sampling was made in average tree in each plot, based on diameter at breast height (DBH, in three trees six month-old per treatment. Within all treatments and your replicates, installed one subplot with long 3.0 m by three corn-rows as wide, where the plants were harvested in stem, leaf, grain, cob and straw. With the exception of Ca, which was more concentrated in the bark fraction and Mg and B in the bark and leaves, the other nutrients in Eucalyptus urograndis, so in monoculture much in mixed stands, contained higher concentration just in leaves. The grain component has the highest concentrations of nitrogen and phosphorus, as straw and cob have the highest potassium concentration and the leaf component has the largest concentrations of other nutrients. The forest species did not influence significantly the levels of nutrients in components of aboveground biomass of maize.

  2. allelopathic effects of eucalyptus tereticornis on phaseolus vulgaris

    African Journals Online (AJOL)

    user

    1. ALLELOPATHIC EFFECTS OF EUCALYPTUS TERETICORNIS ON PHASEOLUS. VULGARIS SEEDLINGS. Sale, F.A.. Department of Forestry and Wildlife, Faculty of ..... Sale, F.A. (2009). Allelopathic influence of Acacia auriculiformis. Eucalyptus citriodora and Gliricidia sepium on germination, growth and yield of millet.

  3. Qualidade de juntas coladas com lâminas de madeira oriundas de três regiões do tronco de Eucalyptus grandis, Eucalyptus saligna e Pinus elliottii Quality of wood joints glued with wood veneers from three trunk regions of Eucalyptus grandis, Eucalyptus saligna and Pinus elliottii

    Directory of Open Access Journals (Sweden)

    Benedito Rocha Vital

    2006-08-01

    Full Text Available Este experimento teve como objetivo avaliar a resistência de juntas coladas formadas pelas combinações de lâminas provenientes de três posições no tronco da madeira de Eucalyptus grandis, Eucalyptus saligna e Pinus elliottii. Foram empregados adesivos à base de poliacetato de vinila de média e alta viscosidade e resorcinol-formaldeído nas gramaturas de 150 g/m², em face simples para o poliacetato de vinila de média e alta viscosidades e 300 g/m², em face dupla, para o adesivo resorcinólico. O teor médio de umidade das lâminas, no momento da colagem, foi igual a 14%. Os valores médios mais elevados de resistência ao cisalhamento foram obtidos nas juntas produzidas com madeira de Eucalyptus saligna, coladas com adesivos de poliacetato de média viscosidade e resorcinol-formaldeído. A maior porcentagem de falha profunda na madeira foi obtida em juntas de madeira de Pinus elliottii, unidas com adesivo de poliacetato de alta viscosidade, seguidas das juntas de Eucalyptus grandis e coladas com adesivo de poliacetato de média viscosidade. As combinações de lâminas oriundas das seguintes posições no tronco: medula e casca, intermediária e casca e casca e casca resultaram em linhas de cola com maiores resistências ao cisalhamento.The objective of this work was to evaluate the shear strength of glued wood joints from pith, outer and intermediary wood of Eucalyptus saligna, Eucalyptus grandis and Pinus elliottii. High and medium viscosity polyvinyl acetate and resorcinol-phenol adhesives were applied at spread rate of 150 g/m² in single line and at spread rate of 300 g/m² in double glue line for the resorcinolic adhesive. The mean wood moisture content was 14%. Higher shear strength was obtained with Eucalyptus saligna veneer glued with medium viscosity polyvinyl resorcinolic adhesive. The highest percentage of wood failure was found on Pinus elliottii veneer glued with high viscosity polyvinyl acetate adhesive followed by

  4. Toxicity and repellency of essential oils to Zabrotes subfasciatus (Boheman (Coleoptera, Chrysomelidae, Bruchinae in Phaseolus vulgaris L Toxicidade e repelência de óleos essenciais a Zabrotes subfasciatus (Boheman (Coleoptera, Chrysomelidae, Bruchinae em grãos de Phaseolus vulgaris L

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    Solange Maria de França

    2012-09-01

    Full Text Available The effects of tangerine (Phaseolus vulgaris Blanco, lemon (Citrus medica limonum Lush, pear orange (Citrus sinensis L. Osbeck, red copaiba (Copaifera langsdorffii Desf., rosemary (Baccharis dracunculifolia De Candole, Eucalyptus (Eucalyptus globulus Labillardière and E. citriodora Hook, lemongrass (Cymbopogon citratus Stapf. and citronella (Cimbopogon nardus Linnaeus oils at several concentrations on Zabrotes subfasciatus (Boheman were studied. In toxicity tests, grains of Phaseolus vulgaris L. cv. Rajadinho were impregnated with oils and infested with adults of Z. subfasciatus up to 24 hours old. All tested oils were effective in reducing the viable egg-laying and adult emergence of this pest, in function of the concentrations used, highlighting E. citriodora and E. globulus oils which caused 100% effectiveness from 0.5 mL Kg-1 concentration. In repellency tests, two arenas consisting of plastic containers, connected symmetrically to a central box by two plastic tubes were used. In one of the boxes, untreated beans were placed and on the other ones beans treated with each oil concentration were used. In the central box, five couples of Z. subfasciatus were released. Grains of P. vulgaris treated with oils of E. citriodora, C. citratus and C. oleifera reduced the attraction percentage of Z. subfasciatus adults, while the E. globulus increased this percentage. The percentages of reduced viable eggs ranged from 17.9% (C. medica limonum to 93.3% (C. nardus, while the reduction on the number of emerged insects was 23.9% and 95.9%, respectively for these same oils.Estudaram-se os efeitos dos óleos de tangerina 'Cravo' (Phaseolus vulgaris Blanco, limão-siciliano (Citrus medica limonum Lush, laranja 'Pêra' (Citrus sinensis L. Osbeek, copaíba-vermelha (Copaifera langsdorffii Desf., alecrim-do-campo (Baccharis dracunculifolia De Candole, eucalipto (Eucalyptus globulus Labillardière e Eucalyptus citriodora Hook, capim-santo (Cymbopogon citratus

  5. Tolerance of Anadenanthera peregrina to Eucalyptus camaldulensis and Eucalyptus grandis essential oil as condition for mixed plantation

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    Neimar de Freitas Duarte

    2012-06-01

    Full Text Available With the purpose of selecting the species of woody Caatinga for mixed plantations with Eucalyptus spp., the allelophatic effects of E. camaldulensis and E. grandis essential oil were studied on the growth activities of Anadenanthera peregrina. The plants were closed in glass chambers in the presence of volatile oil of E. camaldulensis or E. grandis at the concentration of 13 nl.cm-3. The number of leaves, height and diameter at soil lever were compared before, immediately after and after 30 days. Chlorophyll a and b, carotenoids and dry mass were evaluated after the treatment application. There was no inhibitory effect of E. camaldulensis and E. grandis oils on A. peregrina. E. camaldulensis, which was more adapted to semi-arid conditions, was planted in mixture stands with two native legume species, inoculated with Rhizobium and arbuscular mycorrhizal fungi. E. camaldulensis did not inhibit native species growth after two years of cultivation.

  6. Aluminum-Tolerant Pisolithus Ectomycorrhizas Confer Increased Growth, Mineral Nutrition, and Metal Tolerance to Eucalyptus in Acidic Mine Spoil

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    Louise Egerton-Warburton

    2015-01-01

    Full Text Available Ectomycorrhizal fungi (ECM may increase the tolerance of their host plants to Al toxicity by immobilizing Al in fungal tissues and/or improving plant mineral nutrition. Although these benefits have been demonstrated in in vitro (pure culture or short-term nutrient solution (hydroponic experiments, fewer studies have examined these benefits in the field. This study examined the growth, mineral nutrition, and Al levels in two Eucalyptus species inoculated with three Pisolithus ecotypes that varied in Al tolerance (in vitro and grown in mine spoil in the greenhouse and field. All three ecotypes of Pisolithus improved Eucalyptus growth and increased host plant tolerance to Al in comparison to noninoculated plants. However, large variations in plant growth and mineral nutrition were detected among the Pisolithus-inoculated plants; these differences were largely explained by the functional properties of the Pisolithus inoculum. Seedlings inoculated with the most Al-tolerant Pisolithus inoculum showed significantly higher levels of N, P, Ca, Mg, and K and lower levels of Al than seedlings inoculated with Al-sensitive ecotypes of Pisolithus. These findings indicate an agreement between the fungal tolerance to Al in vitro and performance in symbiosis, indicating that both ECM-mediated mineral nutrient acquisition and Al accumulation are important in increasing the host plant Al tolerance.

  7. Eucalyptus grandis AND Eucalyptus dunnii USE FOR WOOD-CEMENT PANELS MANUFACTURING

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    Setsuo Iwakiri

    2008-03-01

    Full Text Available This research evaluated the potential use of Eucalyptus grandis and Eucalyptus dunnii wood for wood-cement panelsmanufacturing. The boards were manufactured at the density of 1,20 g/cm³, using portland cement as mineral bonding and woodfurnish without treatment, treated in cold water and hot water. The wood furnish of Pinus taeda was used as control. The resultsindicated that it is not necessary to treat E. grandis and E. dunni wood for wood-cement board manufacturing. In relation to woodspecies, the board manufactured with E. dunnii showed lower values of mechanical properties. However, boards manufactured of E.grandis wood showed satisfactory results in comparison to boards of P. taeda and the referenced values of BISON process and otherproducts cited in the pertnent literature, indicating the high potential for wood-cement board manufacture of this tree species.

  8. Eucalyptus cloeziana AS A NEW HOST TO Hylesia paulex (LEPIDOPTERA: SATURNIIDAE IN SOUTHEAST BRAZIL

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    Alexandre Igor Azevedo Pereira

    2009-01-01

    Full Text Available An unidentified Lepidoptera species was found defoliating Eucalyptus cloeziana (Myrtaceae in a cerrado area of Minas Gerais State, Brazil. Pupae of this insect, collected in the area, were brought to the laboratory and maintained in Petri dishes (9.0 cm x 1.5 cm under 25 ± 2oC, relative humidity of 60 ± 10% and 12 hours photophase to obtain adults and eggs. This insect was identified as Hylesia paulex Dognin (Lepidoptera: Saturniidae, and, in that way, the objective of the present study was to register for the first time its herbivory in E. cloeziana plants. Newly-emerged caterpillars were reared in 10 plastic pots (500ml, with 30 caterpillars per pot and fed, daily, with fresh leaves of Eucalyptus cloeziana (Myrtaceae. The egg incubation period of H. paulex was 32.00 ± 1.19 days. The total duration of the seven instars of this insect was 67.83 ± 0.84 days. Hylesia paulex completed its life cycle with E. cloeziana plants, what proves its adaptability to this kind of exotic Myrtaceae in Brazil.

  9. Volume loss as a tool to assess kiln drying of eucalyptus wood

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    Djeison Cesar Batista

    Full Text Available In this study, we aimed to analyze the kiln drying quality of Eucalyptus grandis, Eucalyptus saligna and Eucalyptus dunnii woods with respect to volume loss. Wood from the three species was kiln dried together with the same drying schedule and conditions in a conventional-temperature pilot kiln. Three kinds of volume loss were evaluated: total - from saturated (initial to machined (final condition; shrinkage - from saturated to 10% moisture content; and machining - from 10% moisture content to machined condition. Eucalyptus grandis wood was the most dimensionally stable and presented the smallest volume loss due to shrinkage. Although they had different shrinkage behaviors, Eucalyptus grandis and Eucalyptus saligna woods presented the same drying quality regarding machining and total volume losses. These species can be considered the same for kiln drying. Eucalyptus dunnii wood presented the worst quality in drying, and should not be kiln dried in the same batch with the other species.

  10. Utilization of eucalyptus for bioelectricity production in brazil via fast pyrolysis: a techno-economic analysis

    Science.gov (United States)

    In this study, a process model of a 2000 metric ton per day (MTPD) eucalyptus Tail Gas Reactive Pyrolysis (TGRP) and electricity generation plant was developed and simulated in SimSci Pro/II software for the purpose of evaluating its techno-economic viability in Brazil. Two scenarios were compared b...

  11. Transcriptionally active LTR retrotransposons in Eucalyptus genus are differentially expressed and insertionally polymorphic.

    Science.gov (United States)

    Marcon, Helena Sanches; Domingues, Douglas Silva; Silva, Juliana Costa; Borges, Rafael Junqueira; Matioli, Fábio Filippi; Fontes, Marcos Roberto de Mattos; Marino, Celso Luis

    2015-08-14

    In Eucalyptus genus, studies on genome composition and transposable elements (TEs) are particularly scarce. Nearly half of the recently released Eucalyptus grandis genome is composed by retrotransposons and this data provides an important opportunity to understand TE dynamics in Eucalyptus genome and transcriptome. We characterized nine families of transcriptionally active LTR retrotransposons from Copia and Gypsy superfamilies in Eucalyptus grandis genome and we depicted genomic distribution and copy number in two Eucalyptus species. We also evaluated genomic polymorphism and transcriptional profile in three organs of five Eucalyptus species. We observed contrasting genomic and transcriptional behavior in the same family among different species. RLC_egMax_1 was the most prevalent family and RLC_egAngela_1 was the family with the lowest copy number. Most families of both superfamilies have their insertions occurring Eucalyptus species. Using EST analysis and qRT-PCRs, we observed transcriptional activity in several tissues and in all evaluated species. In some families, osmotic stress increases transcript values. Our strategy was successful in isolating transcriptionally active retrotransposons in Eucalyptus, and each family has a particular genomic and transcriptional pattern. Overall, our results show that retrotransposon activity have differentially affected genome and transcriptome among Eucalyptus species.

  12. Treatment of Upper Respiratory Tract Infections in Primary Care: A Randomized Study Using Aromatic Herbs

    Directory of Open Access Journals (Sweden)

    Eran Ben-Arye

    2011-01-01

    Full Text Available This study is a prospective randomized double-blind controlled trial whose aim was to investigate the clinical effects of aromatic essential oils in patients with upper respiratory tract infections. The trial was conducted in six primary care clinics in northern Israel. A spray containing aromatic essential oils of five plants (Eucalyptus citriodora, Eucalyptus globulus, Mentha piperita, Origanum syriacum, and Rosmarinus officinalisas applied 5 times a day for 3 days and compared with a placebo spray. The main outcome measure was patient assessment of the change in severity of the most debilitating symptom (sore throat, hoarseness or cough. Sixty patients participated in the study (26 in the study group and 34 in the control group. Intention-to-treat analysis showed that 20 minutes following the spray use, participants in the study group reported a greater improvement in symptom severity compared to participants in the placebo group (=.019. There was no difference in symptom severity between the two groups after 3 days of treatment (=.042. In conclusion, spray application of five aromatic plants reported in this study brings about significant and immediate improvement in symptoms of upper respiratory ailment. This effect is not significant after 3 days of treatment.

  13. Low doses of glyphosate enhance growth, CO2 assimilation, stomatal conductance and transpiration in sugarcane and eucalyptus.

    Science.gov (United States)

    Nascentes, Renan F; Carbonari, Caio A; Simões, Plinio S; Brunelli, Marcela C; Velini, Edivaldo D; Duke, Stephen O

    2018-05-01

    Sublethal doses of herbicides can enhance plant growth and stimulate other process, an effect known as hormesis. The magnitude of hormesis is dependent on the plant species, the herbicide and its dose, plant development stage and environmental parameters. Glyphosate hormesis is well established, but relatively little is known of the mechanism of this phenomenon. The objective of this study was to determine if low doses of glyphosate that cause growth stimulation in sugarcane and eucalyptus concomitantly stimulate CO 2 assimilation. Shoot dry weight in both species increased at both 40 and 60 days after application of 6.2 to 20.2 g a.e. ha -1 glyphosate. The level of enhanced shoot dry weight was 11 to 37%, depending on the time after treatment and the species. Concomitantly, CO 2 assimilation, stomatal conductance and transpiration were increased by glyphosate doses similar to those that caused growth increases. Glyphosate applied at low doses increased the dry weight of sugarcane and eucalyptus plants in all experiments. This hormetic effect was related to low dose effects on CO 2 assimilation rate, stomatal conductance and transpiration rate, indicating that low glyphosate doses enhance photosynthesis of plants. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  14. Floral Preference of Melipona eburnea Friese (Hymenoptera: Apidae) in a Colombian Andean Region.

    Science.gov (United States)

    Obregon, D; Nates-Parra, G

    2014-02-01

    Melipona eburnea Friese is a stingless bee kept in some regions of Colombia, where it is reported to be vulnerable to extinction due to habitat disturbance. To contribute to raising conservation strategies, the aim of this study was to identify the floral preferences of this species using melissopalynological analysis. A total of 31 pollen pot samples and 37 honey samples were taken from March 2009 through March 2010 from four colonies in Fusagasuga, Colombia. We found 92 pollen types: 17 from pollen pot samples, 39 from honey samples (indicating the sources of nectar), and 36 in both types of samples. The most frequent pollen types in the pollen pot samples were Myrcia type (100%), Eucalyptus globulus (96.9%), and Fraxinus uhdei (96.9%). The most frequent pollen types in honey samples were E. globulus (97.4%) and Myrcia type (94.9%). The pollen types corresponded mainly to native plants (68%), trees (44.5%), plants whose sexual system is hermaphroditic (56.5%), and plants with inflorescences (76.2%). The most frequent shapes of the flowers were brush-like (type Myrtaceae) and dish-like (type Asteraceae), and the preferred flower colors were white or cream (52.2%). In general, we found that M. eburnea showed a strong preference for trees of the family Myrtaceae to obtain nectar and pollen, including native and introduced species. Some other families are contributing significantly, such as Melastomataceae for pollen collection and Asteraceae for nectar. These results highlight the key plant species for the diet of M. eburnea.

  15. Transpiration and water use efficiency in native chilean and exotic species, a usefull tool for catchment management?

    Science.gov (United States)

    Hervé-Fernández, P.; Oyarzun, C. E.

    2012-04-01

    Land-use and forest cover change play important roles in socio-economic processes and have been linked with water supply and other ecosystem services in various regions of the world. Water yield from watersheds is a major ecosystem service for human activities but has been altered by landscape management superimposed on climatic variability and change. Sustaining ecosystem services important to humans, while providing a dependable water supply for agriculture and urban needs is a major challenge faced by managers of human-dominated or increased antropical effect over watersheds. Since water is mostly consumed by vegetation (i.e: transpiration), which strongly depends on trees physiological characteristics (i.e: foliar area, transpiration capacity) are very important. The quantity of water consumed by plantations is influenced mainly by forest characteristics (species physiology, age and management), catchment water retention capacity and meteorological characteristics. Eventhough in Chile, the forest sector accounts for 3.6% of the gross domestic product (GDP) and 12.5% of total exports (INFOR, 2003), afforestation with fast growing exotic species has ended up being socially and politically questionable because of the supposed impact on the environment and water resources. We present data of trees transpiration and water use efficiency from three headwater catchments: (a) second growth native evergreen forest (Aetoxicon punctatum, Drimys winterii, Gevuina avellana, Laureliopsis philippiana); (b) Eucalyptus globulus plantation, and (c) a mixed native deciduous (Nothofagus obliqua and some evergreen species) forest and Eucalyptus globulus and Acacia melanoxylon plantation located at the Coastal Mountain Range in southern Chile (40°S). Annual transpiration rates ranged from 1.24 ± 0.41 mol•m-2•s-1 (0.022 ± 0.009 L•m-2•s-1) for E. globulus, while the lowest observed was for L. philippiana 0.44 ± 0.31 mol•m-2•s-1 (0.008 ± 0.006 L•m-2•s-1). However

  16. The Commercial Profitability of Growing Hybrid Eucalyptus Clones in The Coast Province, Kenya

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    Balozi Bekuta Kirongo

    2014-04-01

    Full Text Available Due to the current high demand for timber, fuelwood, and building poles and the realization that tree growing may pay dividends in the short and long term, many farmers are planting trees on their farms. Farmers are increasingly planting eucalyptus partly due to the fast growth rates of the hybrid clones as well as the opportunity to earn money within a short time. In this paper we report on the profitability of growing eucalyptus hybrid clones in the coastal region, Kenya. Tree growth and cost data was sourced from farmers in Malindi, Kilifi, and Msambweni. Market information was sourced from hardwares in North and South Coast while tree growth models were used to provide average tree sizes at various ages. Results showed that a farmer could make a net income of upto Kshs.500,000.00 (USD6,250 in 5 years. Farmers in the South Coast (Kwale and Msambweni spent more on transport than their counterparts in the North Coast (near Gede-KEFRI. This, added to the fact that trees in the South Coast (Msambweni grew less compared to those in North Coast meant that farmers in the south made less profits.

  17. Multigene families encode the major enzymes of antioxidant metabolism in Eucalyptus grandis L

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    Felipe Karam Teixeira

    2005-01-01

    Full Text Available Antioxidant metabolism protects cells from oxidative damage caused by reactive oxygen species (ROS. In plants, several enzymes act jointly to maintain redox homeostasis. Moreover, isoform diversity contributes to the fine tuning necessary for plant responses to both exogenous and endogenous signals influencing antioxidant metabolism. This study aimed to provide a comprehensive view of the major classes of antioxidant enzymes in the woody species Eucalyptus grandis. A careful survey of the FORESTs data bank revealed 36 clusters as encoding antioxidant enzymes: six clusters encoding ascorbate peroxidase (APx isozymes, three catalase (CAT proteins, three dehydroascorbate reductase (DHAR, two glutathione reductase (GR isozymes, four monodehydroascorbate reductase (MDHAR, six phospholipid hydroperoxide glutathione peroxidases (PhGPx, and 12 encoding superoxide dismutases (SOD isozymes. Phylogenetic analysis demonstrated that all clusters (identified herein grouped with previously characterized antioxidant enzymes, corroborating the analysis performed. With respect to enzymes involved in the ascorbate-glutathione cycle, both cytosolic and chloroplastic isoforms were putatively identified. These sequences were widely distributed among the different ESTs libraries indicating a broad gene expression pattern. Overall, the data indicate the importance of antioxidant metabolism in eucalyptus.

  18. Notes à La Flore de Corse, XXV

    OpenAIRE

    Jeanmonod, Daniel

    2015-01-01

    These “notes” deal with 110 noteworthy taxa amongst which 21 are new to the island's flora. One of these taxa is native: Reseda alba subsp. hookeri and 20 are introduced: Abies pinsapo, Acer ×coriaceum, Albizia julibrissin, Arundo micrantha, Azolla filiculoides, Bolboschoenus laticarpus, Digitaria ciliaris, Digitaria violascens, Eucalyptus camaldulensis, E. globulus, Genista januensis subsp. lydia, Hedera helix subsp. maroccana, Morus kagayamae, Oenothera rosea, Paspalum vaginatum, Puccinelli...

  19. Chemical composition and fumigant toxicity of the essential oils from 16 species of Eucalyptus against Haematobia irritans (Diptera: Muscidae) adults

    OpenAIRE

    Juan, Laura W.; Lucía Mulas, Alejandro; Zerba, Eduardo N.; Harrand, Leonel; Marco, Martín; Masuh, Hector M.

    2011-01-01

    Oils extracted from various species of Eucalyptus (Eucalyptus badjensis Beuzev & Welch, Eucalyptus badjensis x Eucalyptus nitens, Eucalyptus benthamii variety dorrigoensis Maiden & Cambage, Eucalyptus botryoides Smith, Eucalyptus dalrympleana Maiden, Eucalyptus fastigata Deane & Maiden, Eucalyptus nobilis L.A.S. Johnson & K. D. Hill, Eucalyptus polybractea R. Baker, Eucalyptus radiata ssp. radiata Sieber ex Spreng, Eucalyptus resinifera Smith, Eucalyptus robertsonii Blakely, Eucalyptus rubida...

  20. Control of Passion Fruit Fungal Diseases Using Essential Oils Extracted from Rosemary (Rosmarinus officinalis) and Eucalyptus (Eucalyptus agglomerata) in Egerton University Main Campus Njoro, Kenya.

    Science.gov (United States)

    Waithaka, Paul Njenga; Gathuru, Eliud Mugu; Githaiga, Benson Muriuki; Kimani, Salome Nduta

    2017-01-01

    Growth of fruits which form an important part of human diet has been jeopardized by the many fungal diseases that are present today. This study was conceived to isolate the most common fungal pathogens in passion fruits. Fungi were isolated using potato dextrose agar in addition to characterization using morphological, cultural, and biochemical means. Extraction of essential oils from rosemary ( Rosmarinus officinalis ) and eucalyptus ( Eucalyptus agglomerata ) was done. Before carrying the sensitivity test of essential oils to the fungal isolates, constituents of the essential oils were determined. The most common fungal pathogens isolated from passion fruits were Alternaria spp. (45%), Fusarium spp. (22%), Colletotrichum spp. (17%), and Penicillium spp. (16%). There was a relationship between heating time and yield of essential oils in rosemary ( r = 0.99) and eucalyptus ( r = 0.99). Conversely, there was no significant difference in the amount of essential oils produced by rosemary and eucalyptus ( P = 0.08). Furthermore, there was a significant difference in growth inhibition of the fungal pathogens between essential oils from rosemary and eucalyptus ( P = 0.000438). Fungal pathogens isolated from passion fruits can be controlled using essential oils from rosemary and eucalyptus. The oils need to be produced in large scale.

  1. Interspecific hybridization and inbreeding effect in seed from a Eucalyptus grandis x E. urophylla clonal orchard in Brazil

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    Campinhos Eduardo N.

    1998-01-01

    Full Text Available We used allozyme markers to estimate the amount of natural hybridization between Eucalyptus grandis and E. urophylla in a 7.4-hectare commercial hybrid-seed orchard planted in Espírito Santo, Brazil. This orchard was planted in 1982 using a honeycomb design, with each hexagonal plot containing one E. grandis tree surrounded by six E. urophylla trees. There were 267 replicated hexagonal plots in the orchard. Seeds were harvested from the E. grandis clone only. The multilocus outcrossing rate estimated for the E. grandis clone averaged 70.2%, ranging from 33.0 to 99.0% among individual trees. Contaminant pollination, inferred from progeny genotypes containing alleles not present in the seven parental clones, accounted for 14.4% of the hybrid seed. Contaminant pollen was attributed to neighboring eucalyptus stands isolated from the orchard by a 400-m wide belt of native forest. Inbred and hybrid progenies were identified by their allozyme genotypes and transplanted to the field. Field growth of inbred progeny was 30% lower than that of hybrid plants at two and three years of age.

  2. Biomass of clone of Eucalyptus grandis x urophylla for producing briquettes; Biomassa de clone de Eucalyptus grandis x Eucalyptus urophylla para producao de briquetes

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, Eder Aparecido; Oguri, Guilherme [Universidade Estadual Paulista Julio de Mesquita Filho (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas], e-mail: os_garcias@fca.unesp.br; Lancas, Kleber Pereira [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas. Dept. de Engenharia Rural; Guerra, Saulo Philipe Sebastiao [Universidade Estadual Paulista Julio de Mesquita Filho (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas. Dept. de Gestao e Tecnologia Agroindustrial

    2011-07-01

    The aim of this work was conducted to address forest biomass energy for briquette producing. In an area of dystrophic soil, seedlings of clones of Eucalyptus grandis x E. urophylla were planted in 2008, considering factors spacing and fertilization. The first dosage of fertilizer was 70 g/plant of NPK 6-30-6 and total coverage of 110 g/plant of NPK 20-0-20 with B and Zn. The spacing was 2.8x0.5 m, 2.8x1.0 m, 2.8x1.5 m, 2.8x2.0 m and 2.8x2.5 m. At 18 months, tree samples were collected to evaluate the basic density of wood (BDW), dry biomass of stem, branches and leaves. An assessment of the economic viability of each treatment was based on the sale of briquettes. BDW spacing of 2.8x1.0 m was 0.464 kg/m{sup 3}. The largest biomass of the stem occurred in 2.8x0.5 m spacing, with dosage 3, but economically unviable. The dry biomass of branches was only affected by dosage, reaching 17.68 t/ha in the third dose. Only fertilization was significant for leaf biomass. The highest income in the spacing was 2.8 x1.5 m with dosage 2. (author)

  3. Microarray-based method for the parallel analysis of genotypes and expression profiles of wood-forming tissues in Eucalyptus grandis

    CSIR Research Space (South Africa)

    Barros, E

    2009-05-01

    Full Text Available of Eucalyptus grandis planting stock that exhibit preferred wood qualities is thus a priority of the South African forestry industry. The researchers used microarray-based DNA-amplified fragment length polymorphism (AFLP) analysis in combination with expression...

  4. Biologia do psilídeo-de-concha Glycaspis brimblecombei Moore (Hemiptera, Psyllidae em Eucalyptus spp. Red gum lerp psyllid Glycaspis brimblecombei Moore (Hemiptera, Psylidae biology in Eucalyptus spp.

    Directory of Open Access Journals (Sweden)

    Daniela Cristina Firmino-Winckler

    2009-01-01

    Full Text Available Biologia do psilídeo-de-concha Glycaspis brimblecombei Moore (Hemiptera, Psyllidae em Eucalyptus spp.. Glycaspis brimblecombei Moore também conhecido por psilídeo-de-concha, se caracteriza por ser uma espécie específica ao gênero Eucalyptus L'Her. Este trabalho teve por objetivo determinar o ciclo biológico de G. brimblecombei em Eucalyptus spp. O trabalho foi conduzido em câmara climatizada (BOD, sob a temperatura de 26 °C e fotofase de 12 horas. As espécies de Eucalyptus utilizadas para o experimento foram: Eucalyptus camaldulensis, E. tereticornis, E. urophylla, E. grandis, Corymbia citriodora e um híbrido de E. grandis x E. urophylla ('urograndis'. Inicialmente foram utilizados 100 repetições (ninfas tratamento (espécies de Eucalyptus. As avaliações foram diárias. Os parâmetros biológicos avaliados foram a duração e viabilidade do estágio ninfal, longevidade dos adultos, número de posturas/fêmea, duração do período embrionário, número e viabilidade ovos, longevidade dos adultos e duração do ciclo total. Os resultados obtidos demonstraram que salvo C. citriodora que apresentou ser letal ao desenvolvimento ninfal de G. brimblecombei as demais espécies de Eucalyptus testadas oferecem condições ao desenvolvimento biológico deste psilídeo, sendo que neste trabalho E. camaldulensis mostrou-se a mais adequada.Glycaspis brimblecombei Moore, also known as red gum lerp psyllid, is characterized to be specific to the genus Eucalyptus. This work aimed to evaluate G. brimblecombei biological cycle in Eucalyptus spp. The work was accomplished in acclimatized chamber (BOD, with temperature of 26 °C and photophase of 12 hours. Eucalyptus species used in this study were: Eucalyptus camaldulensis, E. tereticornis, E. urophylla, E. grandis, Corymbia citriodora and E grandis x E. urophylla hybrid ('urograndis'. Initially 100 replications (nymphs per treatment (Eucalyptus species were prepared. The evaluations were daily and

  5. Properties of particleboards fabricated with eucalyptus (Eucalyptus urophylla, parica (Schizolobium amazonicum and vassoura (Sida spp. particles

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    Juliana Jerásio Bianche

    2012-12-01

    Full Text Available The genus Sida may become a promising alternative source for the production of particleboards. Nowadays it is used for manufacturing ropes due to the quality of its fibers, however, there is no research relating the production of particleboards using this lignocelluloses material. This study aimed to evaluate the effect of eucalyptus (Eucalyptus urophylla, paricá (Schizolobium amazonicum and vassoura (Sida spp. on the physical and mechanical properties of particleboard. Particleboard contained 25, 50, 75 and 100% of eucalyptus and paricá or eucalytus and vassoura or paricá an vassoura were fabricated. Two (6% and 8% amounts of urea-formaldehyde adhesive were used. The physical and mechanical properties were determined according to NBR / ABNT 14810-3 (ASSOCIAÇÃO BRASILEIRA DE NORMAS TÉCNICAS - ABNT, 2002. It was observed that increase in resin content in the improved some physical and mechanical properties. It was concluded that the amount of vassoura particles on panels had different effects depending on the species used. The increase in the percentage of vassoura particles in the Eucalyptus panels did not affect the internal bond, screw withdrawal and hardness. Increasing the percentage of vassoura particles on the panels produced with paricá did not affect its hardness and resistance to screw withdrawal. The panels produced with vassoura particle absorbed more water and, consequently, increased the thickness swelling, and therefore is not recommended for use in environments with high humidity. It was concluded that the genus Sida, in general, has potential for particleboard production.

  6. Desenvolvimento do Pinus elliottii e do Eucalyptus tereticornis consorciado com plantas de cobertura, em solos degradados por arenização Growth of Pinus elliottii and Eucalyptus tereticornis associated with cover plants, in degraded soils by arenization

    Directory of Open Access Journals (Sweden)

    Ana Paula Moreira Rovedder

    2008-02-01

    Full Text Available Espécies florestais exóticas têm sido utilizadas para a recuperação de solos degradados, auxiliando no controle da erosão hídrica e eólica. No sudoeste do Rio Grande do Sul, foram testados o Pinus elliotii Engelm. e o Eucalyptus tereticornis Sm., com o objetivo de avaliar o crescimento destas espécies e testar sua capacidade na contenção do fenômeno da arenização. Os tratamentos consistiram do plantio das duas espécies sobre o solo degradado por arenização (SD, sobre o campo nativo (C, sobre o campo nativo associado a plantas de cobertura (C + PC e sobre o solo degradado com plantas de cobertura (SD + PC. Como plantas de cobertura, foram testadas a aveia preta (Avena strigosa Schieb. e um tremoço nativo do ecossistema dos campos sulinos, o Lupinus albescens H. et Arn.. Determinaram-se o percentual de sobrevivência inicial, a altura, o diâmetro à altura do colo (DAC e o fator de produtividade (FP das espécies florestais, aos 30 dias, seis meses e 12 meses após plantio. O experimento foi conduzido em delineamento inteiramente casualizado, e os resultados submetidos ao teste de Duncan, em um nível de 5% de probabilidade de erro. Em relação à sobrevivência inicial, apenas o pínus apresentou diferenças estatísticas, com menor percentual no tratamento C+PC. Os resultados demonstraram que o eucalipto beneficiou-se do consórcio com plantas de cobertura, enquanto o pínus apresentou maior sensibilidade à competição interespecífica.Exotic forest species have been used for soil reclamation, as well as helping in the control of water and wind erosion. In the southwest of Rio Grande do Sul state, Brazil, the Pinus elliotii Engelm. and the Eucalyptus tereticornis Sm., were tested with the objective of evaluating the growth of these species and testing its capacity for reducing the arenization process. The treatments consisted of planting the two species on the degraded soil by arenization (DS, on the native grassfield (C

  7. Eucalyptus pollen allergy and asthma in children: a cross-sectional study in South-East Queensland, Australia.

    Science.gov (United States)

    Gibbs, Jane E M

    2015-01-01

    To investigate Eucalyptus (gum tree) pollen allergy in children in relation to geography, particularly vegetation, and its relationship to asthma. Males (n = 180) and females (n = 200) aged 9 to 14 participated. Some were healthy (asymptomatic), some had asthma, and some had other symptoms associated with atopy. School students were from three urban coastal schools and one school from a nearby semi-rural elevated area (range) near Brisbane, Australia. Coastal and range locations featured different distributions of Myrtaceae family vegetation (including Eucalyptus, Melaleuca, Leptospermum species). Skin prick test (SPT) responses to 15 commercial allergens were compared. As well, responses from coast versus range groups, and 'asthma' (n = 97) versus 'healthy' status (n = 107) groups, were compared. SPT responses (≥3mm wheal diameter) indicate that children with asthma are 31.1 times more likely to be allergic to Eucalyptus pollen extract (OR: 31.1; 95%CI 4.1- 235.7) compared to healthy children. Dust mite (p = .018), Eucalyptus (p = .046) and cockroach (p = .047) allergen SPT responses (wheals ≥3mm) were significantly greater in participants located on the coast versus range as determined by Fisher's Exact Test (α .05). For each location, percentage of positive responses (wheals ≥3mm) was greatest for 'dust mite' (30.9%-46%), 'cockroach' (18.1% -35%) and 'Bermuda grass' (10.6%-19.4%). The results support the hypothesis that proximity to Myrtaceae vegetation is related to positive SPT response and that Eucalyptus is an important allergen for children with asthma. Substantial response to olive allergen, in the absence of olive trees, suggests that the response may be driven by substances in other plants, perhaps Melaleuca quinquenervia, which abounds in coastal areas. Response to Eucalyptus allergen indicates that changes in gardening practice in schools and public areas may be appropriate. The findings pose validity questions regarding the use of some

  8. Eucalyptus pollen allergy and asthma in children: a cross-sectional study in South-East Queensland, Australia.

    Directory of Open Access Journals (Sweden)

    Jane E M Gibbs

    Full Text Available To investigate Eucalyptus (gum tree pollen allergy in children in relation to geography, particularly vegetation, and its relationship to asthma.Males (n = 180 and females (n = 200 aged 9 to 14 participated. Some were healthy (asymptomatic, some had asthma, and some had other symptoms associated with atopy. School students were from three urban coastal schools and one school from a nearby semi-rural elevated area (range near Brisbane, Australia. Coastal and range locations featured different distributions of Myrtaceae family vegetation (including Eucalyptus, Melaleuca, Leptospermum species. Skin prick test (SPT responses to 15 commercial allergens were compared. As well, responses from coast versus range groups, and 'asthma' (n = 97 versus 'healthy' status (n = 107 groups, were compared.SPT responses (≥3mm wheal diameter indicate that children with asthma are 31.1 times more likely to be allergic to Eucalyptus pollen extract (OR: 31.1; 95%CI 4.1- 235.7 compared to healthy children. Dust mite (p = .018, Eucalyptus (p = .046 and cockroach (p = .047 allergen SPT responses (wheals ≥3mm were significantly greater in participants located on the coast versus range as determined by Fisher's Exact Test (α .05. For each location, percentage of positive responses (wheals ≥3mm was greatest for 'dust mite' (30.9%-46%, 'cockroach' (18.1% -35% and 'Bermuda grass' (10.6%-19.4%.The results support the hypothesis that proximity to Myrtaceae vegetation is related to positive SPT response and that Eucalyptus is an important allergen for children with asthma. Substantial response to olive allergen, in the absence of olive trees, suggests that the response may be driven by substances in other plants, perhaps Melaleuca quinquenervia, which abounds in coastal areas.Response to Eucalyptus allergen indicates that changes in gardening practice in schools and public areas may be appropriate. The findings pose validity questions regarding the use of some commercial

  9. Estimativa do tempo de vaporização de toras de Eucalyptus grandis Steaming times estimates for Eucalyptus grandis logs

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    Fred Willians Calonego

    2006-06-01

    Full Text Available O objetivo deste estudo foi adequar o modelo geral de determinação do tempo de vaporização de toras, proposto por Steinhagen et al. (1980, para a madeira de Eucalyptus grandis. Para tanto, foram coletadas toras de 20 a The aim this study was to adjust the general model for determining log steaming time, proposed by Steinhagen et al. (1980, for Eucalyptus grandis wood. In order to do so, logs with diameter from 20 to <25, 25 to <30 and 30 to <35 cm were collected from 14 trees of Eucalyptus grandis derived from the `Horto Florestal' nursery, Manduri, São Paulo. A thermocouple was inserted into each log near its center. The logs were steamed during 20 hours at 90ºC and 100% relative humidity. A data logger recorded the temperatures during the thermal treatment. It was concluded that the Steinhagen et al. (1980 model cannot be directly used for this species in study and corrections factors are proposed for the utilization of the general model for log steaming time, developed by Steinhagen et al. (1980, for Eucalyptus grandis wood.

  10. Production cost of biomasses from eucalyptus and elefant grass for energy

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    Laurent Marie Roger Quéno

    2011-09-01

    Full Text Available This work established the unit energy cost generated from biomass of eucalyptus (Eucalyptus sp. and elephant grass (Pennisetum sp. and applied a sensitivity analysis to verify the influences of factors such as the silviculture of eucalyptus, production volume of each species, the cost of land and the interest rate. It was shown that the treatment of eucalyptus in very short rotation of 2 years with reform of stand every 6 years has a average cost of production higher than the traditional treatment of short rotation of 6 years with reform only at the age of 18. It was also observed that eucalyptus has a Production Cost on average of R$ 4,41 /Gj, lower than the elephant grass which is on average of R$ 5,44/Gj, which however has a higher annual capacity of dry matter production. The elephant grass has the possibility to compete with eucalyptus when a set of conditions is met: discount rate higher than or equal to 8%, High price of land, and elephant grass high volume production, greater than or equal to 35 tonnes of dry matter per hectare and year.

  11. Eucalyptus Tree: A Potential Source of Cryptococcus neoformans in Egyptian Environment

    Directory of Open Access Journals (Sweden)

    Mahmoud Elhariri

    2016-01-01

    Full Text Available In Egypt, the River Red Gum (Eucalyptus camaldulensis is a well-known tree and is highly appreciated by the rural and urban dwellers. The role of Eucalyptus trees in the ecology of Cryptococcus neoformans is documented worldwide. The aim of this survey was to show the prevalence of C. neoformans during the flowering season of E. camaldulensis at the Delta region in Egypt. Three hundred and eleven samples out of two hundred Eucalyptus trees, including leaves, flowers, and woody trunks, were collected from four governorates in the Delta region. Thirteen isolates of C. neoformans were recovered from Eucalyptus tree samples (4.2%. Molecular identification of C. neoformans was done by capsular gene specific primer CAP64 and serotype identification was done depending on LAC1 gene. This study represents an update on the ecology of C. neoformans associated with Eucalyptus tree in Egyptian environment.

  12. Acacia Changes Microbial Indicators and Increases C and N in Soil Organic Fractions in Intercropped Eucalyptus Plantations

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    Arthur P. A. Pereira

    2018-04-01

    Full Text Available Intercropping forest plantations of Eucalyptus with nitrogen-fixing trees can increase soil N inputs and stimulate soil organic matter (OM cycling. However, microbial indicators and their correlation in specific fractions of soil OM are unclear in the tropical sandy soils. Here, we examined the microbial indicators associated with C and N in the soil resulting from pure and intercropped Eucalyptus grandis and Acacia mangium plantations. We hypothesized that introduction of A. mangium in a Eucalyptus plantation promotes changes in microbial indicators and increases C and N concentrations on labile fractions of the soil OM, when compared to pure eucalyptus plantations. We determined the microbial and enzymatic activity, and the potential for C degradation by the soil microbial community. Additionally, we evaluated soil OM fractions and litter parameters. Soil (0–20 cm and litter samples were collected at 27 and 39 months after planting from the following treatments: pure E. grandis (E and A. mangium (A plantations, pure E. grandis plantations with N fertilizer (E+N and an E. grandis, and A. mangium intercropped plantations (E+A. The results showed that intercropped plantations (E+A increase 3, 45, and 70% microbial biomass C as compared to A, E+N, and E, at 27 months after planting. The metabolic quotient (qCO2 showed a tendency toward stressful values in pure E. grandis plantations and a strong correlation with dehydrogenase activity. A and E+A treatments also exhibited the highest organic fractions (OF and C and N contents. A canonical redundancy analysis revealed positive correlations between microbial indicators of soil and litter attributes, and a strong effect of C and N variables in differentiating A and E+A from E and E+N treatments. The results suggested that a significant role of A. mangium enhance the dynamics of soil microbial indicators which help in the accumulation of C and N in soil OF in intercropped E. grandis plantations. Our

  13. Acacia Changes Microbial Indicators and Increases C and N in Soil Organic Fractions in Intercropped Eucalyptus Plantations

    Science.gov (United States)

    Pereira, Arthur P. A.; Zagatto, Maurício R. G.; Brandani, Carolina B.; Mescolotti, Denise de Lourdes; Cotta, Simone R.; Gonçalves, José L. M.; Cardoso, Elke J. B. N.

    2018-01-01

    Intercropping forest plantations of Eucalyptus with nitrogen-fixing trees can increase soil N inputs and stimulate soil organic matter (OM) cycling. However, microbial indicators and their correlation in specific fractions of soil OM are unclear in the tropical sandy soils. Here, we examined the microbial indicators associated with C and N in the soil resulting from pure and intercropped Eucalyptus grandis and Acacia mangium plantations. We hypothesized that introduction of A. mangium in a Eucalyptus plantation promotes changes in microbial indicators and increases C and N concentrations on labile fractions of the soil OM, when compared to pure eucalyptus plantations. We determined the microbial and enzymatic activity, and the potential for C degradation by the soil microbial community. Additionally, we evaluated soil OM fractions and litter parameters. Soil (0–20 cm) and litter samples were collected at 27 and 39 months after planting from the following treatments: pure E. grandis (E) and A. mangium (A) plantations, pure E. grandis plantations with N fertilizer (E+N) and an E. grandis, and A. mangium intercropped plantations (E+A). The results showed that intercropped plantations (E+A) increase 3, 45, and 70% microbial biomass C as compared to A, E+N, and E, at 27 months after planting. The metabolic quotient (qCO2) showed a tendency toward stressful values in pure E. grandis plantations and a strong correlation with dehydrogenase activity. A and E+A treatments also exhibited the highest organic fractions (OF) and C and N contents. A canonical redundancy analysis revealed positive correlations between microbial indicators of soil and litter attributes, and a strong effect of C and N variables in differentiating A and E+A from E and E+N treatments. The results suggested that a significant role of A. mangium enhance the dynamics of soil microbial indicators which help in the accumulation of C and N in soil OF in intercropped E. grandis plantations. Our results are

  14. Acacia Changes Microbial Indicators and Increases C and N in Soil Organic Fractions in Intercropped Eucalyptus Plantations.

    Science.gov (United States)

    Pereira, Arthur P A; Zagatto, Maurício R G; Brandani, Carolina B; Mescolotti, Denise de Lourdes; Cotta, Simone R; Gonçalves, José L M; Cardoso, Elke J B N

    2018-01-01

    Intercropping forest plantations of Eucalyptus with nitrogen-fixing trees can increase soil N inputs and stimulate soil organic matter (OM) cycling. However, microbial indicators and their correlation in specific fractions of soil OM are unclear in the tropical sandy soils. Here, we examined the microbial indicators associated with C and N in the soil resulting from pure and intercropped Eucalyptus grandis and Acacia mangium plantations. We hypothesized that introduction of A. mangium in a Eucalyptus plantation promotes changes in microbial indicators and increases C and N concentrations on labile fractions of the soil OM, when compared to pure eucalyptus plantations. We determined the microbial and enzymatic activity, and the potential for C degradation by the soil microbial community. Additionally, we evaluated soil OM fractions and litter parameters. Soil (0-20 cm) and litter samples were collected at 27 and 39 months after planting from the following treatments: pure E. grandis (E) and A. mangium (A) plantations, pure E. grandis plantations with N fertilizer (E+N) and an E. grandis , and A. mangium intercropped plantations (E+A). The results showed that intercropped plantations (E+A) increase 3, 45, and 70% microbial biomass C as compared to A, E+N, and E, at 27 months after planting. The metabolic quotient ( q CO 2 ) showed a tendency toward stressful values in pure E. grandis plantations and a strong correlation with dehydrogenase activity. A and E+A treatments also exhibited the highest organic fractions (OF) and C and N contents. A canonical redundancy analysis revealed positive correlations between microbial indicators of soil and litter attributes, and a strong effect of C and N variables in differentiating A and E+A from E and E+N treatments. The results suggested that a significant role of A. mangium enhance the dynamics of soil microbial indicators which help in the accumulation of C and N in soil OF in intercropped E. grandis plantations. Our results

  15. Plant and bird diversity in natural forests and in native and exotic plantations in NW Portugal

    Science.gov (United States)

    Proença, Vânia M.; Pereira, Henrique M.; Guilherme, João; Vicente, Luís

    2010-03-01

    Forest ecosystems have been subjected to continuous dynamics between deforestation and forestation. Assessing the effects of these processes on biodiversity could be essential for conservation planning. We analyzed patterns of species richness, diversity and evenness of plants and birds in patches of natural forest of Quercus spp. and in stands of native Pinus pinaster and exotic Eucalyptus globulus in NW Portugal. We analyzed data of forest and non-forest species separately, at the intra-patch, patch and inter-patch scales. Forest plant richness, diversity and evenness were higher in oak forest than in pine and eucalypt plantations. In total, 52 species of forest plants were observed in oak forest, 33 in pine plantation and 28 in eucalypt plantation. Some forest species, such as Euphorbia dulcis, Omphalodes nitida and Eryngium juresianum, were exclusively or mostly observed in oak forest. Forest bird richness and diversity were higher in both oak and pine forests than in eucalypt forest; evenness did not differ among forests. In total, 16 species of forest birds were observed in oak forest, 18 in pine forest and 11 in eucalypt forest. Species such as Certhia brachydactyla, Sitta europaea and Dendrocopos major were common in oak and/or pine patches but were absent from eucalypt stands. Species-area relationships of forest plants and forest birds in oak patches had consistently a higher slope, at both the intra and inter-patch scales, than species-area relationships of forest species in plantations and non-forest species in oak forest. These findings demonstrate the importance of oak forest for the conservation of forest species diversity, pointing the need to conserve large areas of oak forest due to the apparent vulnerability of forest species to area loss. Additionally, diversity patterns in pine forest were intermediate between oak forest and eucalypt forest, suggesting that forest species patterns may be affected by forest naturalness.

  16. Genetic architecture of carbon isotope composition and growth in Eucalyptus across multiple environments.

    Science.gov (United States)

    Bartholomé, Jérôme; Mabiala, André; Savelli, Bruno; Bert, Didier; Brendel, Oliver; Plomion, Christophe; Gion, Jean-Marc

    2015-06-01

    In the context of climate change, the water-use efficiency (WUE) of highly productive tree varieties, such as eucalypts, has become a major issue for breeding programmes. This study set out to dissect the genetic architecture of carbon isotope composition (δ(13) C), a proxy of WUE, across several environments. A family of Eucalyptus urophylla × E. grandis was planted in three trials and phenotyped for δ(13) C and growth traits. High-resolution genetic maps enabled us to target genomic regions underlying δ(13) C quantitative trait loci (QTLs) on the E. grandis genome. Of the 15 QTLs identified for δ(13) C, nine were stable across the environments and three displayed significant QTL-by-environment interaction, suggesting medium to high genetic determinism for this trait. Only one colocalization was found between growth and δ(13) C. Gene ontology (GO) term enrichment analysis suggested candidate genes related to foliar δ(13) C, including two involved in the regulation of stomatal movements. This study provides the first report of the genetic architecture of δ(13) C and its relation to growth in Eucalyptus. The low correlations found between the two traits at phenotypic and genetic levels suggest the possibility of improving the WUE of Eucalyptus varieties without having an impact on breeding for growth. © 2015 CIRAD. New Phytologist © 2015 New Phytologist Trust.

  17. Shifts in the bacterial community composition along deep soil profiles in monospecific and mixed stands of Eucalyptus grandis and Acacia mangium

    Science.gov (United States)

    de Andrade, Pedro Avelino Maia; Bini, Daniel; Durrer, Ademir; Robin, Agnès; Bouillet, Jean Pierre; Andreote, Fernando Dini; Cardoso, Elke Jurandy Bran Nogueira

    2017-01-01

    Our knowledge of the rhizosphere bacterial communities in deep soils and the role of Eucalyptus and Acacia on the structure of these communities remains very limited. In this study, we targeted the bacterial community along a depth profile (0 to 800 cm) and compared community structure in monospecific or mixed plantations of Acacia mangium and Eucalyptus grandis. We applied quantitative PCR (qPCR) and sequence the V6 region of the 16S rRNA gene to characterize composition of bacterial communities. We identified a decrease in bacterial abundance with soil depth, and differences in community patterns between monospecific and mixed cultivations. Sequence analysis indicated a prevalent effect of soil depth on bacterial communities in the mixed plant cultivation system, and a remarkable differentiation of bacterial communities in areas solely cultivated with Eucalyptus. The groups most influenced by soil depth were Proteobacteria and Acidobacteria (more frequent in samples between 0 and 300 cm). The predominant bacterial groups differentially displayed in the monospecific stands of Eucalyptus were Firmicutes and Proteobacteria. Our results suggest that the addition of an N2-fixing tree in a monospecific cultivation system modulates bacterial community composition even at a great depth. We conclude that co-cultivation systems may represent a key strategy to improve soil resources and to establish more sustainable cultivation of Eucalyptus in Brazil. PMID:28686690

  18. Overland flow generation processes in sub-humid Mediterranean forest stands

    Science.gov (United States)

    Ferreira, A. J. D.; Ferreira, C. S. S.; Coelho, C. O. A.; Walsh, R. P. D.; Shakesby, R. A.

    2012-04-01

    Forest soils in north and central Portugal have suffered and continue to suffer major structural changes as a result of forest management techniques, such as clear-felling and as a result of wildfire and rip-ploughing, which is carried out to prepare the ground for planting tree seedlings. In soils that have undergone these changes, the characteristics tend to be different for coniferous plantations, where the root system tends to die when the trees are cut following fire and subsequently may be consumed by fire to form a macropore network, and other types of tree plantations where the root system remains alive and allows regrowth from the sawn tree stumps. Overland flow thresholds decrease sharply as a result of rip-ploughing and forest fires and increase following clear-felling. The time taken for trees to reach maturity after wildfire differs markedly betwen the two main species (Pinus pinaster Aiton and Eucalyptus globulus Labill.) stands. In this paper, overland flow is considered in relation to rainfall, throughfall and throughflow, both in terms of hydrology and hydrochemistry in an attempt to understand overland flow generation mechanisms for a variety of forest land uses (mature pine and eucalyptus, pine seedling regrowth and eucalyptus regrowth from tree stumps, eucalyptus plantations and burned pine). Overland flow generation processes change sharply, even within a single rainfall event, as reflected in the soil hydrological processes and the hydrochemical fingerprints. These effects result from the different contact times for water and soil, which cause differences in the absorption and exhudation processes for the two species

  19. Eucalyptus: an open-source cloud computing infrastructure

    International Nuclear Information System (INIS)

    Nurmi, Daniel; Wolski, Rich; Grzegorczyk, Chris; Obertelli, Graziano; Soman, Sunil; Youseff, Lamia; Zagorodnov, Dmitrii

    2009-01-01

    Utility computing, elastic computing, and cloud computing are all terms that refer to the concept of dynamically provisioning processing time and storage space from a ubiquitous 'cloud' of computational resources. Such systems allow users to acquire and release the resources on demand and provide ready access to data from processing elements, while relegating the physical location and exact parameters of the resources. Over the past few years, such systems have become increasingly popular, but nearly all current cloud computing offerings are either proprietary or depend upon software infrastructure that is invisible to the research community. In this work, we present Eucalyptus, an open-source software implementation of cloud computing that utilizes compute resources that are typically available to researchers, such as clusters and workstation farms. In order to foster community research exploration of cloud computing systems, the design of Eucalyptus emphasizes modularity, allowing researchers to experiment with their own security, scalability, scheduling, and interface implementations. In this paper, we outline the design of Eucalyptus, describe our own implementations of the modular system components, and provide results from experiments that measure performance and scalability of a Eucalyptus installation currently deployed for public use. The main contribution of our work is the presentation of the first research-oriented open-source cloud computing system focused on enabling methodical investigations into the programming, administration, and deployment of systems exploring this novel distributed computing model.

  20. Development and assessment of microarray-based DNA fingerprinting in Eucalyptus grandis.

    Science.gov (United States)

    Lezar, Sabine; Myburg, A A; Berger, D K; Wingfield, M J; Wingfield, B D

    2004-11-01

    Development of improved Eucalyptus genotypes involves the routine identification of breeding stock and superior clones. Currently, microsatellites and random amplified polymorphic DNA markers are the most widely used DNA-based techniques for fingerprinting of these trees. While these techniques have provided rapid and powerful fingerprinting assays, they are constrained by their reliance on gel or capillary electrophoresis, and therefore, relatively low throughput of fragment analysis. In contrast, recently developed microarray technology holds the promise of parallel analysis of thousands of markers in plant genomes. The aim of this study was to develop a DNA fingerprinting chip for Eucalyptus grandis and to investigate its usefulness for fingerprinting of eucalypt trees. A prototype chip was prepared using a partial genomic library from total genomic DNA of 23 E. grandis trees, of which 22 were full siblings. A total of 384 cloned genomic fragments were individually amplified and arrayed onto glass slides. DNA fingerprints were obtained for 17 individuals by hybridizing labeled genome representations of the individual trees to the 384-element chip. Polymorphic DNA fragments were identified by evaluating the binary distribution of their background-corrected signal intensities across full-sib individuals. Among 384 DNA fragments on the chip, 104 (27%) were found to be polymorphic. Hybridization of these polymorphic fragments was highly repeatable (R2>0.91) within the E. grandis individuals, and they allowed us to identify all 17 full-sib individuals. Our results suggest that DNA microarrays can be used to effectively fingerprint large numbers of closely related Eucalyptus trees.

  1. FT overexpression induces precocious flowering and normal reproductive development in Eucalyptus.

    Science.gov (United States)

    Klocko, Amy L; Ma, Cathleen; Robertson, Sarah; Esfandiari, Elahe; Nilsson, Ove; Strauss, Steven H

    2016-02-01

    Eucalyptus trees are among the most important species for industrial forestry worldwide. However, as with most forest trees, flowering does not begin for one to several years after planting which can limit the rate of conventional and molecular breeding. To speed flowering, we transformed a Eucalyptus grandis × urophylla hybrid (SP7) with a variety of constructs that enable overexpression of FLOWERING LOCUS T (FT). We found that FT expression led to very early flowering, with events showing floral buds within 1-5 months of transplanting to the glasshouse. The most rapid flowering was observed when the cauliflower mosaic virus 35S promoter was used to drive the Arabidopsis thaliana FT gene (AtFT). Early flowering was also observed with AtFT overexpression from a 409S ubiquitin promoter and under heat induction conditions with Populus trichocarpa FT1 (PtFT1) under control of a heat-shock promoter. Early flowering trees grew robustly, but exhibited a highly branched phenotype compared to the strong apical dominance of nonflowering transgenic and control trees. AtFT-induced flowers were morphologically normal and produced viable pollen grains and viable self- and cross-pollinated seeds. Many self-seedlings inherited AtFT and flowered early. FT overexpression-induced flowering in Eucalyptus may be a valuable means for accelerating breeding and genetic studies as the transgene can be easily segregated away in progeny, restoring normal growth and form. © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  2. Above-ground dry matter accumulation by Eucalyptus grandis and its relation to standard meteorological data

    International Nuclear Information System (INIS)

    Byrne, G.F.

    1989-01-01

    The relationship between the rate of increase of biomass in some stands of Eucalyptus grandis, growing near Coffs Harbour, N.S.W., Australia, is explored in terms of estimated evapotranspiration and radiation interception, and related to a similar previous study of Pinus radiata. A possible role of method of planting, site slope and site aspect in biomass increase is also discussed

  3. Evolutionary history determines how plant productivity responds to phylogenetic diversity and species richness

    Directory of Open Access Journals (Sweden)

    Mark A. Genung

    2014-03-01

    Full Text Available The relationship between biodiversity and ecosystem function has received a great deal of attention in ecological research and recent results, from re-analyses, suggest that ecosystem function improves with increases in phylogenetic diversity. However, many of these results have been generalized across a range of different species and clades, and plants with different evolutionary histories could display different relationships between biodiversity and ecosystem function. To experimentally test this hypothesis, we manipulated species richness and phylogenetic diversity using 26 species from two subgenera of the genus Eucalyptus (subgenus Eucalyptus and subgenus Symphyomyrtus. We found that plant biomass (a measurement of ecosystem function sometimes, but not always, responded to increases in species richness and phylogenetic diversity. Specifically, Symphyomyrtus plants showed a positive response while no comparable effect was observed for Eucalyptus plants, showing that responses to biodiversity can vary across different phylogenetic groups. Our results show that the impacts of evolutionary history may complicate the relationship between the diversity of plant communities and plant biomass.

  4. A new genomic resource dedicated to wood formation in Eucalyptus

    Directory of Open Access Journals (Sweden)

    Couloux Arnaud

    2009-03-01

    Full Text Available Abstract Background Renowned for their fast growth, valuable wood properties and wide adaptability, Eucalyptus species are amongst the most planted hardwoods in the world, yet they are still at the early stages of domestication because conventional breeding is slow and costly. Thus, there is huge potential for marker-assisted breeding programs to improve traits such as wood properties. To this end, the sequencing, analysis and annotation of a large collection of expressed sequences tags (ESTs from genes involved in wood formation in Eucalyptus would provide a valuable resource. Results We report here the normalization and sequencing of a cDNA library from developing Eucalyptus secondary xylem, as well as the construction and sequencing of two subtractive libraries (juvenile versus mature wood and vice versa. A total of 9,222 high quality sequences were collected from about 10,000 cDNA clones. The EST assembly generated a set of 3,857 wood-related unigenes including 2,461 contigs (Cg and 1,396 singletons (Sg that we named 'EUCAWOOD'. About 65% of the EUCAWOOD sequences produced matches with poplar, grapevine, Arabidopsis and rice protein sequence databases. BlastX searches of the Uniref100 protein database allowed us to allocate gene ontology (GO and protein family terms to the EUCAWOOD unigenes. This annotation of the EUCAWOOD set revealed key functional categories involved in xylogenesis. For instance, 422 sequences matched various gene families involved in biosynthesis and assembly of primary and secondary cell walls. Interestingly, 141 sequences were annotated as transcription factors, some of them being orthologs of regulators known to be involved in xylogenesis. The EUCAWOOD dataset was also mined for genomic simple sequence repeat markers, yielding a total of 639 putative microsatellites. Finally, a publicly accessible database was created, supporting multiple queries on the EUCAWOOD dataset. Conclusion In this work, we have identified a

  5. Parâmetros genéticos cuantitativos em famílias de polinización aberta de Eucalyptus urophylla. The estimation of quantitative genetic parameters in open pollinated progênies of Eucalyptus urophylla.

    Directory of Open Access Journals (Sweden)

    Gabriel Costa ROCHA

    2016-12-01

    Full Text Available La eucaliptocultura en el Brasil representa aproximadamente 5,6 millones de hectáreas plantadas, con una producción media de madera de 39 m³.ha.año para el año 2014. Eucalyptus urophylla se destaca como una de las especies más utilizadas e importantes del género para los programas de mejoramiento en el país. De esta forma, el objetivo del presente trabajo fue estimar los parámetros genéticos para las características silviculturales en un ensayo de progenies de polinización abierta de Eucalyptus urophylla, considerando diferentes edades. El experimento fue implantado en área perteneciente a la empresa Eucatex, localizada en la ciudad de Itatinga/SP en un diseño de bloques al acaso, 20 progenies, nueve repeticiones, cinco plantas por parcela, totalizando 900 plantas. Se realizaron las siguientes evaluaciones: a altura de plantas; b diámetro de planta a la altura del pecho y c volumen de madera en metros cúbicos. La estimación de los parámetros genéticos cuantitativos fue realizado adoptando el procedimiento REML/BLUP. El análisis de desviación mostró diferencias significativas (p < 0,05 entre las progenies en estudio. La heredabilidad media de las progenies (h²mp% para los caracteres altura de planta a los 36 meses de edad (ALT: 96, diámetro a la altura del pecho (DAP: 94 y volumen de madera (VOL: 95 mostró alto control genético para la expresión de los caracteres. Las correlaciones fenotípicas y genéticas presentaron valores altos (70 a 97% realizadas con base al DAP, optimizando el trabajo del mejorador. The eucalypt cultivation in Brazil is approximately 5,6 million hectares planted, with an average wood production of 39 m³/hectares per year for 2014. Eucalyptus urophylla stands out as one of the most used and important species of the genus for the improvement programs in the country. Thus, the objective of this study was to estimate genetic parameters for silvicultural characters at a test of open pollinated

  6. Label-Free Quantitative Proteomic Analysis of Puccinia psidii Uredospores Reveals Differences of Fungal Populations Infecting Eucalyptus and Guava.

    Science.gov (United States)

    Quecine, Maria Carolina; Leite, Thiago Falda; Bini, Andressa Peres; Regiani, Thais; Franceschini, Lívia Maria; Budzinski, Ilara Gabriela Frasson; Marques, Felipe Garbelini; Labate, Mônica Teresa Veneziano; Guidetti-Gonzalez, Simone; Moon, David Henry; Labate, Carlos Alberto

    2016-01-01

    Puccinia psidii sensu lato (s.l.) is the causal agent of eucalyptus and guava rust, but it also attacks a wide range of plant species from the myrtle family, resulting in a significant genetic and physiological variability among populations accessed from different hosts. The uredospores are crucial to P. psidii dissemination in the field. Although they are important for the fungal pathogenesis, their molecular characterization has been poorly studied. In this work, we report the first in-depth proteomic analysis of P. psidii s.l. uredospores from two contrasting populations: guava fruits (PpGuava) and eucalyptus leaves (PpEucalyptus). NanoUPLC-MSE was used to generate peptide spectra that were matched to the UniProt Puccinia genera sequences (UniProt database) resulting in the first proteomic analysis of the phytopathogenic fungus P. psidii. Three hundred and fourty proteins were detected and quantified using Label free proteomics. A significant number of unique proteins were found for each sample, others were significantly more or less abundant, according to the fungal populations. In PpGuava population, many proteins correlated with fungal virulence, such as malate dehydrogenase, proteossomes subunits, enolases and others were increased. On the other hand, PpEucalyptus proteins involved in biogenesis, protein folding and translocation were increased, supporting the physiological variability of the fungal populations according to their protein reservoirs and specific host interaction strategies.

  7. Label-Free Quantitative Proteomic Analysis of Puccinia psidii Uredospores Reveals Differences of Fungal Populations Infecting Eucalyptus and Guava.

    Directory of Open Access Journals (Sweden)

    Maria Carolina Quecine

    Full Text Available Puccinia psidii sensu lato (s.l. is the causal agent of eucalyptus and guava rust, but it also attacks a wide range of plant species from the myrtle family, resulting in a significant genetic and physiological variability among populations accessed from different hosts. The uredospores are crucial to P. psidii dissemination in the field. Although they are important for the fungal pathogenesis, their molecular characterization has been poorly studied. In this work, we report the first in-depth proteomic analysis of P. psidii s.l. uredospores from two contrasting populations: guava fruits (PpGuava and eucalyptus leaves (PpEucalyptus. NanoUPLC-MSE was used to generate peptide spectra that were matched to the UniProt Puccinia genera sequences (UniProt database resulting in the first proteomic analysis of the phytopathogenic fungus P. psidii. Three hundred and fourty proteins were detected and quantified using Label free proteomics. A significant number of unique proteins were found for each sample, others were significantly more or less abundant, according to the fungal populations. In PpGuava population, many proteins correlated with fungal virulence, such as malate dehydrogenase, proteossomes subunits, enolases and others were increased. On the other hand, PpEucalyptus proteins involved in biogenesis, protein folding and translocation were increased, supporting the physiological variability of the fungal populations according to their protein reservoirs and specific host interaction strategies.

  8. Isolation of intact sub-dermal secretory cavities from Eucalyptus

    Directory of Open Access Journals (Sweden)

    Goodger Jason QD

    2010-09-01

    Full Text Available Abstract Background The biosynthesis of plant natural products in sub-dermal secretory cavities is poorly understood at the molecular level, largely due to the difficulty of physically isolating these structures for study. Our aim was to develop a protocol for isolating live and intact sub-dermal secretory cavities, and to do this, we used leaves from three species of Eucalyptus with cavities that are relatively large and rich in essential oils. Results Leaves were digested using a variety of commercially available enzymes. A pectinase from Aspergillus niger was found to allow isolation of intact cavities after a relatively short incubation (12 h, with no visible artifacts from digestion and no loss of cellular integrity or cavity contents. Several measurements indicated the potential of the isolated cavities for further functional studies. First, the cavities were found to consume oxygen at a rate that is comparable to that estimated from leaf respiratory rates. Second, mRNA was extracted from cavities, and it was used to amplify a cDNA fragment with high similarity to that of a monoterpene synthase. Third, the contents of the cavity lumen were extracted, showing an unexpectedly low abundance of volatile essential oils and a sizeable amount of non-volatile material, which is contrary to the widely accepted role of secretory cavities as predominantly essential oil repositories. Conclusions The protocol described herein is likely to be adaptable to a range of Eucalyptus species with sub-dermal secretory cavities, and should find wide application in studies of the developmental and functional biology of these structures, and the biosynthesis of the plant natural products they contain.

  9. Energy, chemicals and carbon: future options for the Eucalyptus ...

    African Journals Online (AJOL)

    This paper examines the current Eucalyptus value chain and the possible drivers that may impact upon it. The potential consequences of climate change on the growth and yield of Eucalyptus are discussed and the impact of the security of oil reserves and increased costs of fossil-fuel-derived energy and raw materials upon ...

  10. 40 CFR 180.1241 - Eucalyptus oil; exemption from the requirement of a tolerance.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Eucalyptus oil; exemption from the... Exemptions From Tolerances § 180.1241 Eucalyptus oil; exemption from the requirement of a tolerance. Time-limited exemptions from the requirement of a tolerance are established for residues of eucalyptus oil on...

  11. Metabolic responses and β-carotene production by the unicellular green alga Dunaliella salina exposed to leaf extracts

    Directory of Open Access Journals (Sweden)

    Alireza Einali

    Full Text Available ABSTRACT The present work investigated the effects of aqueous extracts of eucalyptus ( Eucalyptus globulus and elderberry ( Sambucus ebulus leaves on β-carotene productivity in Dunaliella salina, a green microalga. Leaf extracts from eucalyptus have greater amounts of phenolics and flavonoids, as well as greater ferric reducing antioxidant potential than elderberry. The extracts of both species greatly inhibited growth of algal suspensions. However, chlorophyll and β-carotene concentration increased in cells treated with leaf extracts, and the highest values were detected in 1 % eucalyptus and 2 % elderberry extracts. Fresh weight, total sugar, and protein content significantly increased following exposure of cells to different doses of leaf extracts. However, in doses containing more than 2 % eucalyptus, the upward trend for total sugar and protein ceased and remained statistically unchanged. These results suggest that metabolic modifications enable D. salina cells to tolerate the stress induced by the leaf extracts through allocating carbon flux to the synthesis of osmolytes and putative antioxidant molecules (e.g. sugars and β-carotene. Therefore, the use of leaf extracts holds potential to be a promising and effective way to improve D. salina cultivation for β-carotene production and other biotechnological and industrial applications.

  12. Basic density and pulp yield relationship with some chemical parameters in eucalyptus trees Densidade e rendimento em polpa celulósica e sua relação com alguns parâmetros químicos no eucalipto

    Directory of Open Access Journals (Sweden)

    Ana Maria Loureiro da Seca

    2006-12-01

    Full Text Available The objective of this work was to evaluate the use of basic density and pulp yield correlations with some chemical parameters, in order to differentiate an homogeneous eucalyptus tree population, in terms of its potential for pulp production or some other technological applications. Basic density and kraft pulp yield were determined for 120 Eucalyptus globulus trees, and the values were plotted as frequency distributions. Homogenized samples from the first and fourth density quartiles and first and fourth yield quartiles were submitted to total phenols, total sugars and methoxyl group analysis. Syringyl/guaiacyl (S/G and syringaldehyde/vanillin (S/V ratios were determined on the kraft lignins from wood of the same quartiles. The results show the similarity between samples from high density and low yield quartiles, both with lower S/G (3.88-4.12 and S/V (3.99-4.09 ratios and higher total phenols (13.3-14.3 g gallic acid kg-1 . Woods from the high yield quartile are statistically distinguished from all the others because of their higher S/G (5.15 and S/V (4.98 ratios and lower total phenols (8.7 g gallic acid kg-1 . Methoxyl group and total sugars parameters are more adequate to distinguish wood samples with lower density.O objetivo deste trabalho foi avaliar a aplicabilidade de correlações entre rendimento em polpa e densidade básica com alguns parâmetros químicos, para diferenciar uma população homogênea de árvores de eucalipto, em termos de capacidade de produção de polpa celulósica ou de outras aplicações. Determinou-se a densidade básica e o rendimento em polpa celulósica em 120 árvores de Eucalyptus globulus, e os resultados foram representados como distribuição de freqüências. Na madeira das árvores dos primeiro e quarto quartis de densidade e de rendimento, determinaram-se os teores de fenóis totais, açúcares totais e grupos metoxílicos. Nas respectivas ligninas kraft determinaram-se as razões seringilo

  13. 40 CFR 180.1271 - Eucalyptus oil; exemption from the requirement of a tolerance.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Eucalyptus oil; exemption from the... Exemptions From Tolerances § 180.1271 Eucalyptus oil; exemption from the requirement of a tolerance. An exemption from the requirement of tolerance is established for residues of eucalyptus oil in or on honey...

  14. Proton activation studies of changes in mineral composition of eucalyptus obliqua due to phytophthora cinnamomi

    International Nuclear Information System (INIS)

    Chaudri, M.A.; Lee, M.M.; Rouse, J.L.; Weste, G.

    1978-01-01

    As part of a study of disease caused by Phytophthora cinnamomi in native vegetation, the mineral composition of diseased plants was compared with those free from disease, but grown under the same conditions. Young plants of Eucalyptus obliqua, three years old and with well-formed lignotubers, were selected (a) diseased plant from soil containing a high concentration of P. cinnamomi, and (b) unaffected plant from an adjacent area where the soil was free from this pathogen. The plants were ashed and their mineral composition was compared by activation analysis using proton beams from the Melbourne University Cyclotron. Results showed a 70% reduction in iron and 41% in titanium from diseased plants compared with disease-free plants. The reduction in iron is associated with severe chlorosis which occurs as a primary symptom in most plants attacked by this pathogen

  15. Eucalyptus: an open-source cloud computing infrastructure

    Energy Technology Data Exchange (ETDEWEB)

    Nurmi, Daniel; Wolski, Rich; Grzegorczyk, Chris; Obertelli, Graziano; Soman, Sunil; Youseff, Lamia; Zagorodnov, Dmitrii, E-mail: rich@cs.ucsb.ed [Computer Science Department, University of California, Santa Barbara, CA 93106 (United States) and Eucalyptus Systems Inc., 130 Castilian Dr., Goleta, CA 93117 (United States)

    2009-07-01

    Utility computing, elastic computing, and cloud computing are all terms that refer to the concept of dynamically provisioning processing time and storage space from a ubiquitous 'cloud' of computational resources. Such systems allow users to acquire and release the resources on demand and provide ready access to data from processing elements, while relegating the physical location and exact parameters of the resources. Over the past few years, such systems have become increasingly popular, but nearly all current cloud computing offerings are either proprietary or depend upon software infrastructure that is invisible to the research community. In this work, we present Eucalyptus, an open-source software implementation of cloud computing that utilizes compute resources that are typically available to researchers, such as clusters and workstation farms. In order to foster community research exploration of cloud computing systems, the design of Eucalyptus emphasizes modularity, allowing researchers to experiment with their own security, scalability, scheduling, and interface implementations. In this paper, we outline the design of Eucalyptus, describe our own implementations of the modular system components, and provide results from experiments that measure performance and scalability of a Eucalyptus installation currently deployed for public use. The main contribution of our work is the presentation of the first research-oriented open-source cloud computing system focused on enabling methodical investigations into the programming, administration, and deployment of systems exploring this novel distributed computing model.

  16. Methanol production from Eucalyptus wood chips. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Fishkind, H.H.

    1982-06-01

    This feasibility study includes all phases of methanol production from seedling to delivery of finished methanol. The study examines: production of 55 million, high quality, Eucalyptus seedlings through tissue culture; establishment of a Eucalyptus energy plantation on approximately 70,000 acres; engineering for a 100 million gallon-per-day methanol production facility; potential environmental impacts of the whole project; safety and health aspects of producing and using methanol; and development of site specific cost estimates.

  17. Clonal propagation of eucalyptus by tissue culture

    Energy Technology Data Exchange (ETDEWEB)

    Mehra-Palta, A.

    1982-07-01

    Multiple adventitious buds were induced on cotyledons, shoot tips and nodal stem segments of Eucalyptus species cultured on a defined nutrient medium supplemented with the cytokinin zeatin and the auxin indole-3-butyric acid (IBA). The adventitious buds could be recycled on cytokinin medium to produce more buds thus providing the possibility of producing large clones from selected genotypes. The adventitious shoots were rooted in auxin medium and some of the resulting propagules were outplanted in the field. These techniques have the potential for use in the genetic improvement of Eucalyptus. (Refs. 15).

  18. Eucalyptus microfungi known from culture. 2. Alysidiella, Fusculina and Phlogicylindrium genera nova, with notes on some other poorly known taxa

    NARCIS (Netherlands)

    Summerell, B.A.; Groenewald, J.Z.; Carnegie, A.J.; Summerbell, R.C.; Crous, P.W.

    2006-01-01

    Although numerous microfungi have been described from Eucalyptus in recent years, this plant genus remains a rich substrate colonized by numerous undescribed species. In the present study several species and genera of ascomycetes were collected from symptomatic leaves or from leaf litter of this

  19. Flavonoid supplementation affects the expression of genes involved in cell wall formation and lignification metabolism and increases sugar content and saccharification in the fast-growing eucalyptus hybrid E. urophylla x E. grandis.

    Science.gov (United States)

    Lepikson-Neto, Jorge; Nascimento, Leandro C; Salazar, Marcela M; Camargo, Eduardo L O; Cairo, João P F; Teixeira, Paulo J; Marques, Wesley L; Squina, Fabio M; Mieczkowski, Piotr; Deckmann, Ana C; Pereira, Gonçalo A G

    2014-11-19

    Eucalyptus species are the most widely planted hardwood species in the world and are renowned for their rapid growth and adaptability. In Brazil, one of the most widely grown Eucalyptus cultivars is the fast-growing Eucalyptus urophylla x Eucalyptus grandis hybrid. In a previous study, we described a chemical characterization of these hybrids when subjected to flavonoid supplementation on 2 distinct timetables, and our results revealed marked differences between the wood composition of the treated and untreated trees. In this work, we report the transcriptional responses occurring in these trees that may be related to the observed chemical differences. Gene expression was analysed through mRNA-sequencing, and notably, compared to control trees, the treated trees display differential down-regulation of cell wall formation pathways such as phenylpropanoid metabolism as well as differential expression of genes involved in sucrose, starch and minor CHO metabolism and genes that play a role in several stress and environmental responses. We also performed enzymatic hydrolysis of wood samples from the different treatments, and the results indicated higher sugar contents and glucose yields in the flavonoid-treated plants. Our results further illustrate the potential use of flavonoids as a nutritional complement for modifying Eucalyptus wood, since, supplementation with flavonoids alters its chemical composition, gene expression and increases saccharification probably as part of a stress response.

  20. Performance of Australian provenances of Eucalyptus grandis and Eucalyptus saligna in Hawaii

    Science.gov (United States)

    Roger G. Skolmen

    1986-01-01

    Australian provenances of Eucalyptus grandis and E. saligna were compared at four locations on the island of Hawaii to seek seed sources better than those in current use which were introduced earlier from unrecorded locations in Australia. A broad range of latitude and elevation was represented among the provenances. At all four...

  1. Effect of aluminum on metabolism of organic acids and chemical forms of aluminum in root tips of Eucalyptus camaldulensis Dehnh.

    Science.gov (United States)

    Ikka, Takashi; Ogawa, Tsuyoshi; Li, Donghua; Hiradate, Syuntaro; Morita, Akio

    2013-10-01

    Eucalyptus (Eucalyptus camaldulensis) has relatively high resistance to aluminum (Al) toxicity than the various herbaceous plants and model plant species. To investigate Al-tolerance mechanism, the metabolism of organic acids and the chemical forms of Al in the target site (root tips) in Eucalyptus was investigated. To do this, 2-year old rooted cuttings of E. camaldulensis were cultivated in half-strength Hoagland solution (pH 4.0) containing Al (0, 0.25, 0.5, 1.0, 2.5 and 5.0mM) salts for 5weeks; growth was not affected at concentrations up to 2.5mM even with Al concentration reaching 6000μgg(-1) DW. In roots, the citrate content also increased with increasing Al application. Concurrently, the activities of aconitase and NADP(+)-isocitrate dehydrogenase, which catalyze the decomposition of citrate, decreased. On the other hand, the activity of citrate synthase was not affected at concentrations up to 2.5mM Al. (27)Al-NMR spectroscopic analyses were carried out where it was found that Al-citrate complexes were a major chemical form present in cell sap of root tips. These findings suggested that E. camaldulensis detoxifies Al by forming Al-citrate complexes, and that this is achieved through Al-induced citrate accumulation in root tips via suppression of the citrate decomposition pathway. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Distribution of Blue Gum Chalcid, Leptocybe invasa and it's Damage on Eucalyptus in East Africa

    International Nuclear Information System (INIS)

    Mutitu, K.E; Otieno, B.O; Oeba, V.O; Nyeko, P.; Day, R.K

    2007-01-01

    Blue Gum chalcid is a gall-forming wasp, which causes an extensive damage to Eucalyptus. It attacks mostly seedlings and field saplings. It causes damage on it's host by forming massive typical bump-shaped galls on trees canopy, specifically on the leaf midribs, petioles and stems of new growths. The pest was first reported in Kenya and Uganda in 2002 attacking the three major commercially grown Eucalyptus species; Eucalyptus grandis, E. camaldulennsis, E. saligna in East Africa. Eucalyptus is fast growing and widely planted in both plantations and farmers' woodlots. It is important for both industrial and domestic use. It is a major source of income generation for rural poor families. The objective of this study was to determine the spatial distribution, incidence and severity patterns of L. invasa and possible relationship to environment and biophysical factors in East Africa. Over 312 randomly selected stands were assessed for BGC incidence to it's distribution. In each identified stand 60 trees were selected from 3 randomly established plots of 20 trees each and BGC damage/incidence assessed on a 4-point scale. This was to determine the incidence and severity patterns. ANOVA using generalized linear procedures, regression analysis and correlation were done to determine the relationship between the percentage incidence of L. invasa infestation against altitude, age interval, agro-ecological zones and topography. There was widespread of pest in all the districts sampled. There was significant difference (p 0.05) on L. invasa incidence among the three species. The study recommends that. further studies on damage and L. invasa population dynamics relationship to environment and biophysical factors should be conducted

  3. Species climate range influences hydraulic and stomatal traits in Eucalyptus species.

    Science.gov (United States)

    Bourne, Aimee E; Creek, Danielle; Peters, Jennifer M R; Ellsworth, David S; Choat, Brendan

    2017-07-01

    Plant hydraulic traits influence the capacity of species to grow and survive in water-limited environments, but their comparative study at a common site has been limited. The primary aim of this study was to determine whether selective pressures on species originating in drought-prone environments constrain hydraulic traits among related species grown under common conditions. Leaf tissue water relations, xylem anatomy, stomatal behaviour and vulnerability to drought-induced embolism were measured on six Eucalyptus species growing in a common garden to determine whether these traits were related to current species climate range and to understand linkages between the traits. Hydraulically weighted xylem vessel diameter, leaf turgor loss point, the water potential at stomatal closure and vulnerability to drought-induced embolism were significantly ( P Eucalyptus trees has important implications for the limits of species responses to changing environmental conditions and thus for species survival and distribution into the future, and yields new information for physiological models. © The Author 2017. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com

  4. RELATIONSHIP BETWEEN THE ANATOMY AND DRYING IN Eucalyptus grandis X Eucalyptus urophylla WOOD

    Directory of Open Access Journals (Sweden)

    Antônio José Vinha Zanuncio

    Full Text Available ABSTRACT Drying is an important step to using wood and anatomical characteristics influence this process. Thus, the objective of this work was to evaluate the relationship between anatomy and wood drying. Samples with 2 x 2 x 4 cm were obtained from eight Eucalyptus urophylla x Eucalyptus grandis clones at 1.3 m height to evaluate the anatomy and drying in climate chamber during 15 days. Cell wall fraction was established as the parameter that best correlated with drying, the Pearson correlation coefficient between this parameter and moisture was -0.8986, -0.6580, 0.9216, 0.8743, 0.7131 and 0.8727 for saturated wood, and after 1, 2.5, 5, 10 and 15 days of drying, respectively. The frequency and vessel size, as well as the height and width of the rays showed low relation with wood drying. Wood anatomy, mainly the cell wall fraction, influences moisture losses and should be considered in wood drying programs.

  5. Uso de micorrizas e rizóbio em cultivo consorciado de eucalipto e sesbânia: I - Crescimento, absorção e tranferência de nitrogênio entre plantas Use of mycorrhizas and rhizobium in intercropping system of eucalyptus and sesbania: I - Growth, uptake and transfer of nitrogen between plants

    Directory of Open Access Journals (Sweden)

    L. A. Rodrigues

    2003-08-01

    Rhizobium on dry matter production, N uptake and efficiency of use in Eucalyptus grandis and Sesbania virgata plants grown in intercropping systems. The transfer of N from Sesbania virgata to Eucalyptus was also evaluated, using the isotope 15N. The treatments were: with or without inoculation of both plant species with AMF and with or without inoculation of S. virgata with Rhizobium. Plastic pots divided in three compartments (A, B, and C, with a volume of 2 L were used. A and B compartments were separated by a plastic division, while between the B and C compartments a nylon mesh screen (40 mm allowed the penetration of hyphae only, not of plant roots. Root systems of the S. virgata plants were divided between the compartments A and B at planting, and Eucalyptus plants were cultivated in the compartment C. Seven mg kg-1 of 15N-(NH42SO4 with 99% of 15N were applied in compartment A. The plants were grown for 100 days and then harvested. In Eucalyptus plants, the Rhizobium, AMF and AMF + Rhizobium treatments increased the dry matter production by 119, 223, and 209%, respectively, and the N content by 125, 247, and 310%, respectively, in relation to control plants. In S. virgata plants, all treatments inoculated with microorganisms increased the N content and decreased the C/N rate. The efficiency of N use was greater in Eucalyptus plants inoculated with AMF and did not vary with the treatments in sesbania. A transfer of 15N from S. virgata to Eucalyptus plants was observed in all treatments.

  6. Tropical Timbers of the World,

    Science.gov (United States)

    1980-04-01

    Trop. Woods No. 103:1-139. 96 CORDIA SPP. (HAI0-XWlED, DARK- aW CERASCMAUS (BLiP) CANALETE Family: Boraginaceae Other Common Names: Anacahuite, Barfa...tropicaux. Premier Suppl~ment. Publ. Centre Tech. For. Trop. No. 23. 372 Lf4 CORDIA MILLNII and C. PLAT’IYRSA WEST AFRICAN CORDIA Family: Boraginaceae...OE AM Hibiscus elacus and H. tiliaceus BLUEGUM AS Eucalyptus globulus BOCOTE AM Cordia spp. (Hard-wooded, dark-colored Gerascanthus group) BOMBAY

  7. Containers of Attalea funifera fibers to produce eucalyptus seedlings

    Directory of Open Access Journals (Sweden)

    Andrea Vita Reis Mendonça

    2016-09-01

    Full Text Available The aim of this study was to assess the technical feasibility of using biodegradable containers made of fiber waste of Attalea funifera Martius to produce seedling of Eucalyptus camaldulensis Dehnh. The work was carried out in three stages: manufacture of piassava fiber containers, seedling production and field simulation. The experiment of seedling production was in completely randomized design, with two treatments (polyethylene tube and biodegradable container and 10 repetitions, with 64 seedlings per repetition. After 93 days, seedlings were evaluated based on quality variables. The simuation of initial growth of seedlings in the field consisted in planting seedlings in containers of 11L, in completely randomized design, with three treatments: seedlings produced in polyethylene tubes; seedlings produced in biodegradable containers, without removal of the container during planting; and seedlings produced in biodegradable containers, with removal of the container at planting, with ten repetitions, with one seedling by repetition. The biodegradable container withstood the production cycle and resulted in seedlings within acceptable standards quality. The use of biodegradable container, made of palm fibers, waived the removal of this vessel in the final planting.

  8. Volume loss as a tool to assess kiln drying of eucalyptus wood

    OpenAIRE

    Batista,Djeison Cesar; Klitzke,Ricardo Jorge; Rocha,Márcio Pereira da; Muñiz,Graciela Inez Bolzon de; Batista,Tharcia Ribeiro

    2013-01-01

    In this study, we aimed to analyze the kiln drying quality of Eucalyptus grandis, Eucalyptus saligna and Eucalyptus dunnii woods with respect to volume loss. Wood from the three species was kiln dried together with the same drying schedule and conditions in a conventional-temperature pilot kiln. Three kinds of volume loss were evaluated: total - from saturated (initial) to machined (final) condition; shrinkage - from saturated to 10% moisture content; and machining - from 10% moisture content...

  9. GENERAL EQUATIONS OF CARBONIZATION OF EUCALYPTUS SPP KINETIC MECHANISMS

    Directory of Open Access Journals (Sweden)

    Túlio Jardim Raad

    2006-06-01

    Full Text Available In the present work, a set of general equations related to kinetic mechanism of wood compound carbonization: hemicelluloses, cellulose and lignin was obtained by Avrami-Eroffev and Arrhenius equations and Thermogravimetry of Eucalyptus cloeziana, Eucalyptus camaldulensis, Corymbia citriodora, Eucalyptus urophylla and Eucalyptus grandis samples, TG-Isothermal and TG-Dynamic. The different thermal stabilities and decomposition temperature bands of those species compounds were applied as strategy to obtain the kinetic parameters: activation energy, exponential factor and reaction order. The kinetic model developed was validated by thermogravimetric curves from carbonization of others biomass such as coconut. The kinetic parameters found were - Hemicelluloses: E=98,6 kJmol, A=3,5x106s-1 n=1,0; - Cellulose: E=182,2 kJmol, A=1,2x1013s-1 n=1,5; - Lignin: E=46,6 kJmol, A=2,01s-1 n=0,41. The set of equations can be implemented in a mathematical model of wood carbonization simulation (with heat and mass transfer equations with the aim of optimizing the control and charcoal process used to produce pig iron.

  10. Clonal differences in log end splitting in Eucalyptus grandis in ...

    African Journals Online (AJOL)

    This paper discusses the juvenile–mature correlation of log end splitting among Eucalyptus grandis clones from two trials and how differences in splitting relate to differences in wood density, pith-to-bark gradient and growth rate. Two approximately 20-year-old Eucalyptus grandis clonal trials at Bergvliet plantation were ...

  11. Methanol production from eucalyptus wood chips. Attachment IV. Health and safety aspects of the eucalypt biomass to methanol energy system

    Energy Technology Data Exchange (ETDEWEB)

    Fishkind, H.H.

    1982-06-01

    The basic eucalyptus-to-methanol energy process is described and possible health and safety risks are identified at all steps of the process. The toxicology and treatment for exposure to these substances are described and mitigating measures are proposed. The health and safety impacts and risks of the wood gasification/methanol synthesis system are compared to those of the coal liquefaction and conversion system. The scope of this report includes the health and safety risks of workers (1) in the laboratory and greenhouse, where eucalyptus seedlings are developed, (2) at the biomass plantation, where these seedlings are planted and mature trees harvested, (3) transporting these logs and chips to the refinery, (4) in the hammermill, where the logs and chips will be reduced to small particles, (5) in the methanol synthesis plant, where the wood particles will be converted to methanol, and (6) transporting and dispensing the methanol. Finally, the health and safety risks of consumers using methanol is discussed.

  12. Identification of a STOP1-like protein in Eucalyptus that regulates transcription of Al tolerance genes.

    Science.gov (United States)

    Sawaki, Yoshiharu; Kobayashi, Yuriko; Kihara-Doi, Tomonori; Nishikubo, Nobuyuki; Kawazu, Tetsu; Kobayashi, Masatomo; Kobayashi, Yasufumi; Iuchi, Satoshi; Koyama, Hiroyuki; Sato, Shigeru

    2014-06-01

    Tolerance to soil acidity is an important trait for eucalyptus clones that are introduced to commercial forestry plantations in pacific Asian countries, where acidic soil is dominant in many locations. A conserved transcription factor regulating aluminum (Al) and proton (H⁺) tolerance in land-plant species, STOP1 (SENSITIVE TOPROTON RHIZOTOXICITY 1)-like protein, was isolated by polymerase chain reaction-based cloning, and then suppressed by RNA interference in hairy roots produced by Agrobacterium rhizogenes-mediated transformation. Eucalyptus STOP1-like protein complemented proton tolerance in an Arabidopsis thaliana stop1-mutant, and localized to the nucleus in a transient assay of a green fluorescent protein fusion protein expressed in tobacco leaves by Agrobacterium tumefaciens-mediated transformation. Genes encoding a citrate transporting MULTIDRUGS AND TOXIC COMPOUND EXTRUSION protein and an orthologue of ALUMINUM SENSITIVE 3 were suppressed in transgenic hairy roots in which the STOP1 orthologue was knocked down. In summary, we identified a series of genes for Al-tolerance in eucalyptus, including a gene for STOP1-like protein and the Al-tolerance genes it regulates. These genes may be useful for molecular breeding and genomic selection of elite clones to introduce into acid soil regions. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  13. ALTERNATIVES TO IMPROVE HYBRIDIZATION EFFICIENCY IN Eucalyptus BREEDING PROGRAMS

    Directory of Open Access Journals (Sweden)

    Roselaine Cristina Pereira

    2002-01-01

    Full Text Available Simple and quick hybridization procedures and ways to keep pollen grains viable for long periods are sought in plant breeding programs to provide greater work flexibility. The presentstudy was carried out to assess the efficiency of pollinations made shortly after flower emasculationand the viability of stored pollen from Eucalyptus camaldulensis and Eucalyptus urophylla clones cultivated in Northwestern Minas Gerais State. Controlled pollinations were carried out at zero, one,three, five and seven days after emasculation. Hybridization efficiency was assessed by thepercentage of viable fruits, number of seeds produced per fruit, percentage of viable seeds and also bycytological observation of the pollen development along the style. Flower buds from clones of the twospecies were collected close to anthesis to assess the viability of pollen grain storage. Pollen was thencollected and stored in a freezer (-18oC for 1, 2 and 3 months. Pollen assessed was carried out by invitro and in vivo germination tests. The efficiency of the pollinations varied with their delay and alsobetween species. The greatest pollination efficiency was obtained when they were carried out on thethird and fifth day after emasculation, but those performed simultaneously with emasculationproduced enough seeds to allow this practice in breeding programs. The decrease in pollen viabilitywith storage was not sufficiently significant to preclude the use of this procedure in artificialhybridization.

  14. Root damage by insects reverses the effects of elevated atmospheric CO2 on Eucalypt seedlings.

    Directory of Open Access Journals (Sweden)

    Scott N Johnson

    Full Text Available Predicted increases in atmospheric carbon dioxide (CO2 are widely anticipated to increase biomass accumulation by accelerating rates of photosynthesis in many plant taxa. Little, however, is known about how soil-borne plant antagonists might modify the effects of elevated CO2 (eCO2, with root-feeding insects being particularly understudied. Root damage by insects often reduces rates of photosynthesis by disrupting root function and imposing water deficits. These insects therefore have considerable potential for modifying plant responses to eCO2. We investigated how root damage by a soil-dwelling insect (Xylotrupes gideon australicus modified the responses of Eucalyptus globulus to eCO2. eCO2 increased plant height when E. globulus were 14 weeks old and continued to do so at an accelerated rate compared to those grown at ambient CO2 (aCO2. Plants exposed to root-damaging insects showed a rapid decline in growth rates thereafter. In eCO2, shoot and root biomass increased by 46 and 35%, respectively, in insect-free plants but these effects were arrested when soil-dwelling insects were present so that plants were the same size as those grown at aCO2. Specific leaf mass increased by 29% under eCO2, but at eCO2 root damage caused it to decline by 16%, similar to values seen in plants at aCO2 without root damage. Leaf C:N ratio increased by >30% at eCO2 as a consequence of declining leaf N concentrations, but this change was also moderated by soil insects. Soil insects also reduced leaf water content by 9% at eCO2, which potentially arose through impaired water uptake by the roots. We hypothesise that this may have impaired photosynthetic activity to the extent that observed plant responses to eCO2 no longer occurred. In conclusion, soil-dwelling insects could modify plant responses to eCO2 predicted by climate change plant growth models.

  15. Determination and statistical analysis of trace element and active constituent concentrations in the medicinal plant Eucalyptus camaldulensis Dehnh (E. rostratus Schlecht)

    International Nuclear Information System (INIS)

    Kanias, G.D.; Kilikoglou, V.; Tsitsa, E.; Loukis, A.

    1993-01-01

    In the leaves of the medicinal plant Eucalyptus camaldulensis Dehnh (E. rostratus Schlecht) collected from different sampling areas of Greece the trace elements antimony, cesium, chromium, cobalt, iron, europium, rubidium scandium, strontium, thorium and zinc were determined by Instrumental Neutron Activation Analysis. In the same samples, the essential oil was determined by steam distillation and the percent relative composition of the essential oil in 1,8-cineole, p-cymene, α-pinene by gas liquid chromatography. Also the refractive index of the essential oil was determined by a refractometer. Statistical analysis included the calculation of the correlation coefficient. Multiple correlation and cluster analysis was applied to all analytical data. The results showed that the trace elements iron, chromium, cobalt and zinc are correlated with the variation of the concentration of essential oil in the examined plant. These four elements along with rubidium and essential oil content could be used for the separation of the samples into groups related to the sampling areas. Statistically significant correlation between active constituents and some trace elements and a linear negative correlation between 1,8-cineole and refractive index were found. (author) 13 refs.; 2 figs.; 2 tabs

  16. CANONICAL CORRELATION OF PHYSICAL AND CHEMICAL CHARACTERISTICS OF THE WOOD OF Eucalyptus grandis AND Eucalyptus saligna CLONES

    Directory of Open Access Journals (Sweden)

    Paulo Fernando Trugilho

    2003-01-01

    Full Text Available The analysis of canonical correlation measures the existence and the intensity of the association between two groups of variables. The research objectified to evaluate thecanonical correlation between chemical and physical characteristics and fiber dimensional ofwood of Eucalyptus grandis and Eucalyptus saligna clones, verifying the interdependenceamong the groups of studied variables. The analysis indicated that the canonical correlationswere high and that in two cases the first and second pair were significant at the level of 1% ofprobability. The analysis of canonical correlation showed that the groups are notindependent. The intergroup associations indicated that the wood of high insoluble lignin contentand low ash content is associated with the high radial and tangential contraction and highbasic density wood.

  17. Prospects of Apicultural Entrepreneurship in Coastal Districts of Eastern India: A Melissopalynological Evaluation

    Science.gov (United States)

    Upadhyay, Debasis; Bhattacharya, Swapan; Ferguson, David K.; Bera, Subir

    2014-01-01

    A melissopalynological analysis of fifty-one natural honey samples (twenty four spring, fifteen summer and twelve winter) collected during 2010–2011 from two east-coastal districts (20020/ to 22011/ N, 82039/ to 87001/ E) of Orissa, India was performed. Out of 37 unifloral samples found 25 were contributed by Apis cerana indica, seven by A. dorsata and the remaining five by A. florea. Out of 14 multifloral samples five were contributed by A. cerana indica, five by A. dorsata and the remaining four by A. florea. Principal component analysis confirmed the palynological classification of the unifloral honey samples. Eighty-two bee-plant taxa belonging to forty four families were recovered. The predominant nectariferous taxa of the spring season were Acanthus ilicifolius, Avicennia marina, Bruguiera gymnorrhiza, Cocos nucifera, Eucalyptus globulus, Phoenix paludosa, Pongamia pinnata, Prosopis juliflora, Sonneratia apetala and Syzygium cumini. In the summer the predominant nectariferous taxa were Borassus flabellifer, C. nucifera, E. globulus, Syzygium cumini, Terminalia arjuna, Aegiceras corniculatum, P. paludosa and Sonneratia apetala while those of the winter were Brassica nigra, Coriandrum sativum, Zizyphus jujuba, Alstonia scholaris, E. globulus and Bruguiera gymnorrhiza. Very low (<0.09) HDE/P for 98% of the samples and absence of toxic palynotaxa assure that these honeys are suitable for human consumption. Quite extended honey flow period with spring and summer as best forage seasons for the honeybees and occurrence of 82% of these honeys with APC Group II, III and IV justify the sustainability of the present study area for establishing moderate to large-scale apicultural entrepreneurship. This should improve the socio-economic status of the people of this region. PMID:24740144

  18. Prospects of apicultural entrepreneurship in coastal districts of eastern India: a melissopalynological evaluation.

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    Debasis Upadhyay

    Full Text Available A melissopalynological analysis of fifty-one natural honey samples (twenty four spring, fifteen summer and twelve winter collected during 2010-2011 from two east-coastal districts (20(020/ to 22(011/ N, 82(039/ to 87(001/ E of Orissa, India was performed. Out of 37 unifloral samples found 25 were contributed by Apis cerana indica, seven by A. dorsata and the remaining five by A. florea. Out of 14 multifloral samples five were contributed by A. cerana indica, five by A. dorsata and the remaining four by A. florea. Principal component analysis confirmed the palynological classification of the unifloral honey samples. Eighty-two bee-plant taxa belonging to forty four families were recovered. The predominant nectariferous taxa of the spring season were Acanthus ilicifolius, Avicennia marina, Bruguiera gymnorrhiza, Cocos nucifera, Eucalyptus globulus, Phoenix paludosa, Pongamia pinnata, Prosopis juliflora, Sonneratia apetala and Syzygium cumini. In the summer the predominant nectariferous taxa were Borassus flabellifer, C. nucifera, E. globulus, Syzygium cumini, Terminalia arjuna, Aegiceras corniculatum, P. paludosa and Sonneratia apetala while those of the winter were Brassica nigra, Coriandrum sativum, Zizyphus jujuba, Alstonia scholaris, E. globulus and Bruguiera gymnorrhiza. Very low (<0.09 HDE/P for 98% of the samples and absence of toxic palynotaxa assure that these honeys are suitable for human consumption. Quite extended honey flow period with spring and summer as best forage seasons for the honeybees and occurrence of 82% of these honeys with APC Group II, III and IV justify the sustainability of the present study area for establishing moderate to large-scale apicultural entrepreneurship. This should improve the socio-economic status of the people of this region.

  19. Effects of an inducible aiiA gene on disease resistance in Eucalyptus urophylla × Eucalyptus grandis.

    Science.gov (United States)

    Ouyang, L J; Li, L M

    2016-08-01

    N-acyl-homoserine lactones (AHLs) are metabolites of mostly gram-negative bacteria and are critical signaling molecules in bacterial quorum-sensing systems. At threshold concentrations, AHLs can activate the expression of pathogenic genes and induce diseases. Therefore, reducing AHL concentrations is a key point of disease control in plants. AHL-lactonase, which is expressed by aiiA, is widespread in Bacillus sp and can hydrolyze AHLs. In the present study, we cloned aiiA from Bacillus subtilis by PCR. A plant expression vector of aiiA was constructed and name Pcam-PPP3-aiiA, in which expression of aiiA was controlled by the pathogen-inducible plant promoter PPP3. The recombinant plasmid was transferred into Eucalyptus × urophylla × E. grandis by an Agrobacterium-mediated transformation. PCR and Southern blotting showed that aiiA was successfully integrated into the E. urophylla × E. grandis genome and its expression was induced by Ralstonia solanacearum 12 h after inoculation, as shown by reverse transcription-PCR. The transcription efficacy of aiiA increased 43.88-, 30.65-, and 18.95-fold after inoculation with R. solanacearum, Erwinia carotovora ssp. zeae (Sabet) and Cylindrocladium quinqueseptatum, respectively as shown by RT-real-time PCR. Transgenic E.urophylla × E.grandis expressing the AIIA protein exhibited significantly enhanced disease resistance compared to non-transgenic plants by delaying the onset of wilting and reducing the disease index.

  20. Scientific Basis for Sustainable Management of Eucalyptus and Populus as Short-Rotation Woody Crops in the U.S.

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    Eric D. Vance

    2014-05-01

    Full Text Available Short rotation woody crops (SRWC, fast growing tree species that are harvested on short, repeated intervals, can augment traditional fiber sources. These crops have economic and environmental benefits stemming from their capability of supplying fiber on a reduced land base in close proximity to users and when sensitive sites cannot be accessed. Eucalyptus and Populus appear to be genera with the greatest potential to provide supplemental fiber in the U.S. Optimal productivity can be achieved through practices that overcome site limitations and by choosing the most appropriate sites, species, and clones. Some Eucalyptus species are potentially invasive, yet field studies across multiple continents suggest they are slower to disperse than predicted by risk assessments. Some studies have found lower plant and animal diversity in SRWC systems compared to mature, native forests, but greater than some alterative land uses and strongly influenced by stand management, land use history, and landscape context. Eucalyptus established in place of grasslands, arable lands, and, in some cases, native forests can reduce streamflow and lower water tables due to higher interception and transpiration rates but results vary widely, are scale dependent, and are most evident in drier regions.

  1. Variación genética en plántulas de Eucalyptus spp.

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    H. C. Martínez Hernández

    2001-01-01

    Full Text Available En este estudio se evaluó el crecimiento y acumulación de biomasa en plántulas de 7 meses de edad pertenecientes a 42 familias de polinización libre de tres especies de Eucalyptus (E. globulus, E. resinifera, y E. rudis. El ensayo se estableció en condiciones de vivero con germoplasma colectado en plantaciones establecidas en la cuenca del Valle de México. Los resultados indicaron la existencia de variación significativa (P≤0.05 entre especies y entre familias dentro de especies para las variables altura, diámetro, número de hojas y número de ramas, aunque no en el área foliar. A nivel de especies se encontraron diferencias significativas en todas las variables relacionadas con la acumulación y distribución de biomasa, excepto en el peso seco de las hojas. También se encontró una variación significativa (P≤0.01 a nivel de familias dentro de especies en el peso seco del tallo, el peso seco de hojas y la biomasa aérea mientras que el peso seco de las ramas y la biomasa de raíz no presentaron una variación significativa entre las familias. El componente de variación de familias dentro de especies aportó del 2 al 12 % de la variación fenotípica total, mientras que las especies contribuyeron sólo del 0.5 a 7.5 de esta variación. El mayor porcentaje de variación se presentó dentro de parcelas, con valores de 80 a 90 % del total en todas las variables de crecimiento y acumulación de biomasa de las plántulas.

  2. Essential oils and Beauveria bassiana against Dermanyssus gallinae (Acari: Dermanyssidae): Towards new natural acaricides.

    Science.gov (United States)

    Immediato, Davide; Figueredo, Luciana Aguiar; Iatta, Roberta; Camarda, Antonio; de Luna, Rafaela Lira Nogueira; Giangaspero, Annunziata; Brandão-Filho, Sinval Pinto; Otranto, Domenico; Cafarchia, Claudia

    2016-10-15

    Essential oils (EOs) and entomopathogenic fungi such as Beauveria bassiana (Bb) strains have the potential to be used as alternative insecticides and acaricides for controlling ectoparasites as Dermanyssus gallinae. These compounds have some limitations in their use: the acaricidal effect of EOs is rapid, but short-lived, whilst that of Bb is delayed, but long-lived. To evaluate the effect of both compounds combined against D. gallinae, the non-toxic dose of Eucalyptus globulus, Eucalyptus citriodora, Thymus vulgaris and Eugenia caryophyllata essential oils were firstly calculated for "native" strains of Bb. Subsequently, the effects of the combination of selected EOs with Bb against nymph and adult poultry red mites (PRMs) was assessed. EO concentrations ranging from 0.0015 to 8% v/v (i.e., nine double dilutions) were used to evaluate their effect on germination, sporulation and vegetative growth rates of native strains of Bb. A total of 1440 mites (720 nymphs and 720 adults) were divided into three-treated group (TGs) and one control group (CG). In TGs, mites were exposed to Bb in combination with the selected EO (TG1), EO alone (TG2) or Bb (TG3) alone. In the CG, mites were exposed to 0.1% tween 80 plus EO solvent (CG). E. globulus and E. citriodora were toxic for Bb in concentrations higher than 0.2% and 0.003% respectively, whilst E. caryophyllata and T. vulgaris were toxic at all concentrations tested against Bb. Based on the results of the toxicity assays against Bb, E. globulus was chosen to be tested as acaricide resulting non-toxic for Bb at concentration lower than 0.4%. Increased mortality of D. gallinae adults was recorded in TG1 than those in other TGs from 4days post-infection (T+4DPI). A 100% mortality of D. gallinae was recorded in adults at T+9DPI and at T+10DPI in nymphs in TG1 and later than T+11DPI in the other TGs. Used in combination with E. globulus, Bb displayed an earlier acaricidal effect towards both haematophagous D. gallinae stages

  3. Pretreatment of Eucalyptus in biphasic system for furfural production and accelerated enzymatic hydrolysis.

    Science.gov (United States)

    Zhang, Xiudong; Bai, Yuanyuan; Cao, Xuefei; Sun, Runcang

    2017-08-01

    Herein, an efficient biphasic pretreatment process was developed to improve the production of furfural (FF) and glucose from Eucalyptus. The influence of formic acid and NaCl on FF production from xylose in water and various biphasic systems was investigated. Results showed that the addition of formic acid and NaCl significantly promoted the FF yield, and the biphasic system of MIBK (methyl isobutyl ketone)/water exhibited the best performance for FF production. Then the Eucalyptus was pretreated in the MIBK/water system, and a maximum FF yield of 82.0% was achieved at 180°C for 60min. Surface of the pretreated Eucalyptus became relatively rough and loose, and its crystallinity index increased obviously due to the removal of hemicelluloses and lignin. The pretreated Eucalyptus samples showed much higher enzymatic hydrolysis rates (26.2-70.7%) than the raw Eucalyptus (14.5%). Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Antioxidant and Antiacetylcholinesterase Activities of Some Commercial Essential Oils and Their Major Compounds

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    Smail Aazza

    2011-09-01

    Full Text Available The commercial essential oils of Citrus aurantium L., Cupressus sempervirens L., Eucalyptus globulus Labill., Foeniculum vulgare Mill. and Thymus vulgaris L., isolated by steam distillation by a company of Morocco were evaluated in terms of in vitro antioxidant activity through several methods. In vitro acetylcholinesterase inhibitory activity was also determined. Citrus limon (L. Burm. f. oil was also studied, but it was obtained by peel expression. The best antioxidant was T. vulgaris oil, independent of the method used, mainly due to the presence of the phenolic monoterpenes thymol and carvacrol, which when studied as single compounds also presented the best activities. Concerning the acetylcholinesterase inhibition activity, E. globulus was the most effective. Nevertheless its main components 1,8-cineole and limonene were not the most active, a feature that corresponded to d-3-carene.

  5. Stand-volume estimation from multi-source data for coppiced and high forest Eucalyptus spp. silvicultural systems in KwaZulu-Natal, South Africa

    Science.gov (United States)

    Dube, Timothy; Sibanda, Mbulisi; Shoko, Cletah; Mutanga, Onisimo

    2017-10-01

    Forest stand volume is one of the crucial stand parameters, which influences the ability of these forests to provide ecosystem goods and services. This study thus aimed at examining the potential of integrating multispectral SPOT 5 image, with ancillary data (forest age and rainfall metrics) in estimating stand volume between coppiced and planted Eucalyptus spp. in KwaZulu-Natal, South Africa. To achieve this objective, Partial Least Squares Regression (PLSR) algorithm was used. The PLSR algorithm was implemented by applying three tier analysis stages: stage I: using ancillary data as an independent dataset, stage II: SPOT 5 spectral bands as an independent dataset and stage III: combined SPOT 5 spectral bands and ancillary data. The results of the study showed that the use of an independent ancillary dataset better explained the volume of Eucalyptus spp. growing from coppices (adjusted R2 (R2Adj) = 0.54, RMSEP = 44.08 m3/ha), when compared with those that were planted (R2Adj = 0.43, RMSEP = 53.29 m3/ha). Similar results were also observed when SPOT 5 spectral bands were applied as an independent dataset, whereas improved volume estimates were produced when using combined dataset. For instance, planted Eucalyptus spp. were better predicted adjusted R2 (R2Adj) = 0.77, adjusted R2Adj = 0.59, RMSEP = 36.02 m3/ha) when compared with those that grow from coppices (R2 = 0.76, R2Adj = 0.46, RMSEP = 40.63 m3/ha). Overall, the findings of this study demonstrated the relevance of multi-source data in ecosystems modelling.

  6. Epidermal structure of some important forest plants

    Energy Technology Data Exchange (ETDEWEB)

    Farooqui, P

    1981-10-01

    Eucalyptus species are a dominant feature of the vegetation of India, largely planted in forest areas for timber and oil. The wood is used on a large scale in the paper and pulp industries. According to one researcher, Eucalyptus is represented by 605 species in the world, of which over a hundred species are cultivated in India. As they look very similar to each other, with few exceptions, it is difficult to distinguish them when not in flower. The structure of the leaf epidermis in 8 species of Eucalyptus has been studied. It is found that such studies may be useful for demarcating the different species. A guideline to the species studied is provided. (Refs. 13).

  7. Glycosylation of Vanillin and 8-Nordihydrocapsaicin by Cultured Eucalyptus perriniana Cells

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    Naoji Kubota

    2012-05-01

    Full Text Available Glycosylation of vanilloids such as vanillin and 8-nordihydrocapsaicin by cultured plant cells of Eucalyptus perriniana was studied. Vanillin was converted into vanillin 4-O-b-D-glucopyranoside, vanillyl alcohol, and 4-O-b-D-glucopyranosylvanillyl alcohol by E. perriniana cells. Incubation of cultured E. perriniana cells with 8-nor- dihydrocapsaicin gave 8-nordihydrocapsaicin 4-O-b-D-glucopyranoside and 8-nordihydro- capsaicin 4-O-b-D-gentiobioside.

  8. Aceptación y uso de herbolaria en medicina familiar Acceptance and use of therapeutic medical plants in family medical care

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    Genaro Armando Taddei-Bringas

    1999-05-01

    Full Text Available OBJETIVO. Explorar el grado de utilización de las plantas medicinales entre la población usuaria y el personal de salud en una unidad urbana de medicina familiar del Instituto Mexicano del Seguro Social. MATERIAL Y MÉTODOS. Se realizó un estudio transversal descriptivo. Se diseñó y validó un cuestionario que exploró aceptación y utilización. Se aplicó a 60 médicos familiares, a una muestra aleatoria de 130 trabajadores de la Unidad de Medicina Familiar y a otra de 264 usuarios-pacientes de la misma. El porcentaje de respuesta fue de 78%. RESULTADOS. Se encontró que 83% de los médicos aceptan la herbolaria y 75% la utilizan como recurso terapéutico. Entre el personal de salud, la aceptación y la utilización fue de 100% y, en el caso de los usuarios, 92% la aceptan y 90% la utilizan. Las diferencias entre grupos son significativas (pOBJECTIVE. To explore the degree of usage of therapeutic medical plants among the patients, physicans and health workers in a local Family Mediccal Care Unit of the Mexican Institute of Social Security (IMSS. MATERIAL AND METHODS. A transversal descriptive study was performed. A questionnaire focusing on two variables was designed and validated. It was applied to 60 family physicians, a randomized sample of 130 health workers and another of 264 patients of the Family Mediccal Care Unit. Response percentage was 78%. RESULTS. The study found that 83% of family physicians accept the therapeutic use of herbal medicine; moreover, 75% use it as a therapeutic resource. Among health workers, acceptance and use was 100%, while in patients the level of acceptance was of 92% and of use it was 90%. Differences between groups are significant (p<0.05. The more frequently used plants are Gordolobo (Gnaphalium sp., Eucalyptus (Eucalyptus sp., probably E. globulus, spearmint (Mentha sp., camomile (Matricaria chamomilla and prickly pear cladodes (the vegetative parts of the prickly pear, Opuntia sp. Probably Opuntia

  9. Post-fire diversity and abundance in pine and eucalipt stands in Portugal: effects of biogeography, topography, forest type and post-fire management

    OpenAIRE

    Maia, P.; Keizer, J.; Vasques, A.; Abrantes, N.; Roxo, L.; Fernandes, P.; Ferreira, A.; Moreira, F.

    2014-01-01

    This study concerned the mid-term regeneration of the woody understory vegetation of pure and mixed stands of Pinus pinaster Ait. and Eucalyptus globulus Labill. in northern and central Portugal following wildfires in 2005 and 2006. Pine and eucalypt stands are the most widespread and most fire-prone forest types in Portugal. The main aim was to investigate the importance of biogeography, topography, forest type and post-fire management operations in explaining the patterns in shr...

  10. Efectos de plantaciones abandonadas de especies exóticas y nativas sobre la regeneración natural de un bosque montano en Colombia

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    Jaime Cavelier

    1999-12-01

    Full Text Available Se realizaron censos de vegetación durante 1994 en una parcela de 0.1 ha. en cada una de las plantaciones abandonadas de Pinus radiata, Cupressus lusitanica, Eucalyptus globulus, Alnus acuminata y en un bosque secundario montano alto en los Andes centrales de Colombia. También fueron recolectados datos de las propiedades físicas y químicas de suelos superficiales. El bosque de regeneración natural tiene el mayor número de especies (33 seguido por las plantaciones de E. globulus (26 y A. acuminata (16. Las plantaciones abandonadas de P. radiata y C. lusitanica, tuvieron sólo tres especies. Entre el bosque de regeneración natural y la plantación de E. globulus hubo solamente once especies en común y solamente siete con la plantación de A. acuminata. Todas estas especies, con excepción de tres, son arbustos del sotobosque dispersados por viento o aves. En el sotobosque de la plantación de P. radiata había una regeneración abundante del árbol nacional de Colombia, Ceroxylum quindiuensis. La altura de los árboles y el área basal fueron significativamente mayores en las plantaciones de P. radiata y C. lusitanica que en el bosque de regeneración natural. De las variables ambientales y biológicas medidas en este estudio, la acumulación de acículas bajo las plantaciones de P. radiata y C. lusitanica y la alta biomasa de raíces finas bajo la plantación de C. lusitanica, podrían ser los principales factores limitantes para el establecimiento de un número mayor de especies del bosque nativo. Las propiedades químicas de los suelos varían ampliamente y no hubo diferencias coherentes entre los suelos bajo especies exóticas y nativas.Vegetation surveys were carried out during 1994 in 0.1. ha plots in abandoned plantations of Pinus radiata, Cupressus lusitanica, Eucalyptus globulus, Alnus acuminata and in a secondary upper montane rain forest in the Central Andes of Colombia. The regeneration forest had the higher number of plant

  11. ACTIVIDAD INSECTICIDA DE ACEITES ESENCIALES DE DOS ESPECIES DE Eucalyptus SOBRE Rhyzopertha dominica Y SU EFECTO EN ENZIMAS DIGESTIVAS DE PROGENIES

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    Ramiro Reyes-Guzmán

    2012-01-01

    proteolítica de las progenies; sin embargo, solamente E. globulus ocasionó la muerte de éstas (P < 0.05. Al incrementar el volumen de aceite de E. camadulensis, la actividad proteolítica del insecto aumentó, incluso al mayor tiempo de exposición. Los resultados de este estudio muestran que la fracción volátil de los aceites esenciales de eucalipto, especialmente la de E. globulus, es un agente insecticida efectivo para el control de R. dominica en trigo almacenado.

  12. Nanofibrillated cellulose (CNF) from eucalyptus sawdust as a dry strength agent of unrefined eucalyptus handsheets.

    Science.gov (United States)

    Vallejos, María Evangelina; Felissia, Fernando Esteban; Area, María Cristina; Ehman, Nanci Vanesa; Tarrés, Quim; Mutjé, Pere

    2016-03-30

    Nanofibrillated cellulose has been obtained from the cellulosic fraction of eucalyptus sawdust. The fractionation process involved the partial removal of hemicelluloses and lignin. CNF was obtained using TEMPO oxidation with NaOCl in basic medium followed by mechanical homogenization. The obtained CNF was subsequently used as a dry strength agent on unbleached unrefined eucalyptus pulp. The addition of 3, 6 and 9 wt.% of CNF increased lineally the tensile index of handsheets to about 55 N mg(-1) at 35°SR, compatible with papermachine runnability. The other mechanical properties also increased substantially, and porosity decreased moderately. The estimated specific surface and average diameter of these CNF were 60 m(2)g(-1), and of 41.0 nm, respectively. The addition of 9 wt.% of CNF produced an increase in mechanical strength, equivalent to that produced by PFI refining at 1600 revolutions. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. QUALIDADE DA SECAGEM CONVENCIONAL CONJUNTA DA MADEIRA DE CLONES DE TRÊS ESPÉCIES DE Eucalyptus sp.

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    Djeison Cesar Batista

    2015-01-01

    Full Text Available ABSTRACT The Brazilian industry commonly dries different species of eucalypt in a same load because of the difficulty of identifying the species, hybrids and clones, and also by optimizing the use of the kiln. However, this practice is not recommended in the literature. The aim of this work was to verify the quality of grouped conventional drying of Eucalyptus saligna , Eucalyptus grandis and Eucalyptus dunnii from clonal plantations. To this end, three loads were dried with the three species together in a pilot conventional dry kiln. The criteria used to analyze the quality of dried wood were final moisture content, end checks, cupping, collapse, moisture gradient and drying stresses. Eucalyptus grandis showed the best quality of drying, followed by Eucalyptus saligna , and both species have potential to be kiln dried together. Eucalyptus dunnii had the worst quality of drying, and should not be kiln dried together with Eucalyptus grandis and Eucalyptus saligna. The basic density and shrinkage were good parameters for the expected quality of the drying of eucalyptus species, in which less dense and more dimensionally stable species had better quality of drying. The drying schedule used must be modified to further drying of these species, especially regarding to uniformity and conditioning periods, which were considered insufficient.

  14. In vitro anticancer properties of selected Eucalyptus species.

    Science.gov (United States)

    Bhuyan, Deep Jyoti; Sakoff, Jennette; Bond, Danielle R; Predebon, Melanie; Vuong, Quan V; Chalmers, Anita C; van Altena, Ian A; Bowyer, Michael C; Scarlett, Christopher J

    2017-08-01

    In spite of the recent advancements in oncology, the overall survival rate for pancreatic cancer has not improved over the last five decades. Eucalypts have been linked with cytotoxic and anticancer properties in various studies; however, there is very little scientific evidence that supports the direct role of eucalypts in the treatment of pancreatic cancer. This study assessed the anticancer properties of aqueous and ethanolic extracts of four Eucalyptus species using an MTT assay. The most promising extracts were further evaluated using a CCK-8 assay. Apoptotic studies were performed using a caspase 3/7 assay in MIA PaCa-2 cells. The aqueous extract of Eucalyptus microcorys leaf and the ethanolic extract of Eucalyptus microcorys fruit inhibited the growth of glioblastoma, neuroblastoma, lung and pancreatic cancer cells by more than 80% at 100 μg/mL. The E. microcorys and Eucalyptus saligna extracts showed lower GI 50 values than the ethanolic Eucalyptus robusta extract in MIA PaCa-2 cells. Aqueous E. microcorys leaf and fruit extracts at 100 μg/mL exerted significantly higher cell growth inhibition in MIA PaCa-2 cells than other extracts (p  0.05) were observed in aqueous E. microcorys leaf (86.05 ± 4.75 μg/mL) and fruit (64.66 ± 15.97 μg/mL) and ethanolic E. microcorys leaf (79.30 ± 29.45 μg/mL) extracts in MIA PaCa-2 cells using the CCK-8 assay. Caspase 3/7-mediated apoptosis and morphological changes of cells were also witnessed in MIA PaCa-2 cells after 24 h of treatment with the extracts. This study highlighted the significance of E. microcorys as an important source of phytochemicals with efficacy against pancreatic cancer cells. Further studies are warranted to purify and structurally identify individual compounds and elucidate their mechanisms of action for the development of more potent and specific chemotherapeutic agents for pancreatic cancer.

  15. Variation in natural durability of seven Eucalyptus grandis x Eucalyptus urophylla hybrid clones

    Science.gov (United States)

    F.J.N. Franca; T.S.F.A. Franca; R.A Arango; B.M. Woodward; G.B. Vidaurre

    2017-01-01

    Programs aimed at developing clones of hybrid trees are commonly established in Brazil to meet the demands of various forest-based industries. These programs have continually improved the quality of eucalyptus wood, which has the potential to reduce deforestation by lowering demand for other high-value species. This is particularly true in the lumber market, but little...

  16. Larvicidal potential of essential oils against Musca domestica and Anopheles stephensi.

    Science.gov (United States)

    Chauhan, Nitin; Malik, Anushree; Sharma, Satyawati; Dhiman, R C

    2016-06-01

    The larvicidal activity of Mentha piperita, Cymbopogan citratus (lemongrass), Eucalyptus globulus and Citrus sinensis (orange) essential oils and their combinations was evaluated against Musca domestica (housefly) and Anopheles stephensi (mosquitoes) through contact toxicity assay. Among all the tested essential oils/combinations, Me. piperita was found to be the most effective larvicidal agent against Mu. domestica and An. stephensi with LC50 values of 0.66 μl/cm(2) and 44.66 ppm, respectively, after 48 h. The results clearly highlighted that the addition of mentha oil to other oils (1:1 ratio) improved their larvicidal activity. The order of effectiveness of essential oils/combinations indicated that the pattern for An. stephensi follows the trend as mentha > mentha + lemongrass > lemongrass > mentha + eucalyptus > eucalyptus > mentha + orange > orange and for Mu. domestica as mentha > mentha + lemongrass > lemongrass > mentha + orange > orange > mentha + eucalyptus > eucalyptus. The images obtained from scanning electron microscopy (SEM) analysis indicated the toxic effect of Me. piperita as the treated larvae were observed to be dehydrated and deformed. This study demonstrates the effectiveness of tested essential oils/combinations against the larval stages of Mu. domestica and An. stephensi and has the potential for development of botanical formulations.

  17. Eucalyptus water use greater than rainfall input - possible explanation from southern India

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    I. R. Calder

    1997-01-01

    Full Text Available Hydrological and silvicultural studies carried out in southern India on the effects of plantations of Eucalyptus and other fast growing exotic tree species have determined the impacts of these plantations on water resources, erosion, soil nutrient status and growth rates at sites of differing rainfall and soil depth in Karnataka. Whilst providing new information on these issues, the studies also raised two important questions: what was the explanation for the anomalous result that the water use of 3400 mm from Eucalyptus plantations at Hosakote over a three year period exceeded the rainfall of 2100 mm over the same period and why were growth rates of woodlots on most farmer's fields higher than those of plantations on land owned by the Karnataka Forest Department? The records of the soil moisture depletion patterns under these plantations from the day of planting provide the basis for the answers to both questions: i whilst roots are penetrating into deeper soil layers, they are able to extract from a reservoir of water additional to that available from the rainfall each year, ii farmer's land on which short rooted agricultural crops have been grown previously is likely to have a much higher soil water status than land previously under forest or scrub vegetation. These new studies have also established that the development of the drying front under the Eucalyptus camaldulensis plantations is very rapid, indicating average root extension rates in excess of 2.5 m per year, whilst those under Tectona grandis and Artocarpus heterophyllus advanced at approximately half the rate. These results have obvious implications for the long term sustainability of growth rates from these plantations and the recharge of groundwater. The authors believe that this study may be the first to report neutron probe soil moisture depletion observations, from the date of planting, beneath tree plantations in a dry climate. The extent to which the roots were able to

  18. Eucalyptus water use greater than rainfall input - possible explanation from southern India

    Science.gov (United States)

    Calder, I. R.; Rosier, P. T. W.; Prasanna, K. T.; Parameswarappa, S.

    Hydrological and silvicultural studies carried out in southern India on the effects of plantations of Eucalyptus and other fast growing exotic tree species have determined the impacts of these plantations on water resources, erosion, soil nutrient status and growth rates at sites of differing rainfall and soil depth in Karnataka. Whilst providing new information on these issues, the studies also raised two important questions: what was the explanation for the anomalous result that the water use of 3400 mm from Eucalyptus plantations at Hosakote over a three year period exceeded the rainfall of 2100 mm over the same period and why were growth rates of woodlots on most farmer's fields higher than those of plantations on land owned by the Karnataka Forest Department? The records of the soil moisture depletion patterns under these plantations from the day of planting provide the basis for the answers to both questions: i) whilst roots are penetrating into deeper soil layers, they are able to extract from a reservoir of water additional to that available from the rainfall each year, ii) farmer's land on which short rooted agricultural crops have been grown previously is likely to have a much higher soil water status than land previously under forest or scrub vegetation. These new studies have also established that the development of the drying front under the Eucalyptus camaldulensis plantations is very rapid, indicating average root extension rates in excess of 2.5 m per year, whilst those under Tectona grandis and Artocarpus heterophyllus advanced at approximately half the rate. These results have obvious implications for the long term sustainability of growth rates from these plantations and the recharge of groundwater. The authors believe that this study may be the first to report neutron probe soil moisture depletion observations, from the date of planting, beneath tree plantations in a dry climate. The extent to which the roots were able to penetrate raises the

  19. ECONOMIC EVALUATION OF Eucalyptus grandis PLANTATION FOR CELLULOSE PRODUCTION

    Directory of Open Access Journals (Sweden)

    Antonio Donizette de Oliveira

    2008-03-01

    Full Text Available The aims of this research were: to analyze the economic feasibility of planting eucalyptus for producing wood pulp,considering various site index and two spacings; to analyze the economic effects regarding the profitability of the forest activity indifferent distances from the industry and changes on discount rate, wood price, transportation costs, minimum profitable diameter oflogs and the length of the logs. A biometric model for making wood volume prognosis was developed, using data of a trial ofEucalyptus grandis stands 19 and 103 months old. The prognosis started at the age zero, considering logs of 2.5 and 6.0 m of lenghtand the minimum diameter varying from 4 to 10 cm, in intervals of 2 cm. Net Present Worth (NPW was used as the economic decisioncriterium, considering an infinite horizon. The main conclusions were: reducing the minimum profitable diameter and the length ofthe logs are good strategies to increase wood utilization and profit; plantations located in less productive lands are economicallyunfeasible; the cost of transportation has significant effect on the profitability of the forest activity and must be analyzed carefully atthe moment of defining the location of new plantations; small variations on wood sales price may cause big alterations on theprofitability of the forest activity, suggesting that the improvement of the wood quality together with other decisions that may increasewood price are alternatives that may render the plantations in less productive areas profitable.

  20. Dual RNA-sequencing of Eucalyptus nitens during Phytophthora cinnamomi challenge reveals pathogen and host factors influencing compatibility

    Directory of Open Access Journals (Sweden)

    Febe Elizabeth Meyer

    2016-03-01

    Full Text Available Damage caused by Phytophthora cinnamomi Rands remains an important concern on forest tree species. The pathogen causes root and collar rot, stem cankers and dieback of various economically important Eucalyptus spp. In South Africa, susceptible cold tolerant Eucalyptus plantations have been affected by various Phytophthora spp. with P. cinnamomi considered one of the most virulent. The molecular basis of this compatible interaction is poorly understood. In this study, susceptible Eucalyptus nitens plants were stem inoculated with P. cinnamomi and tissue was harvested five days post inoculation. Dual RNA-sequencing, a technique which allows the concurrent detection of both pathogen and host transcripts during infection, was performed. Approximately 1% of the reads mapped to the draft genome of P. cinnamomi while 78% of the reads mapped to the Eucalyptus grandis genome. The highest expressed P. cinnamomi gene in planta was a putative crinkler effector (CRN1. Phylogenetic analysis indicated the high similarity of this P. cinnamomi CRN1 to that of Phytophthora infestans. Some CRN effectors are known to target host nuclei to suppress defense. In the host, over 1400 genes were significantly differentially expressed in comparison to mock inoculated trees, including suites of pathogenesis related (PR genes. In particular, a PR-9 peroxidase gene with a high similarity to a Carica papaya PR-9 ortholog previously shown to be suppressed upon infection by Phytophthora palmivora was down-regulated two-fold. This PR-9 gene may represent a cross-species effector target during P. cinnamomi infection. This study identified pathogenicity factors, potential manipulation targets and attempted host defense mechanisms activated by E. nitens that contributed to the susceptible outcome of the interaction.

  1. Management of Eucalyptus plantations influence small mammals’ density: evidences from Southern Europe

    NARCIS (Netherlands)

    Teixeira, D; Carrilho, M; Mexia, T; Kobel, M; Ferreira Dos Santos, M.J.; Santos-Reis, Margarida; Rosalino, Luis Miguel

    2017-01-01

    Forestry plantations, and particularly those of exotic Eucalyptus, are important man-made systems in Europe, and especially in Portugal, where these represent now the largest fraction of forested areas. Eucalyptus plantations may have impacts on vertebrate communities in Europe; however, these have

  2. Asociacion de leveduras del genero Cryptococcus con especies de Eucalyptus en Santafe de Bogota Isolation of Cryptococcus sp. associated with Eucalyptus trees in Santafé de Bogota

    Directory of Open Access Journals (Sweden)

    A. Duarte

    1994-04-01

    Full Text Available El aislamiento de Cryptococcus neoformans var. gattii, serotipo B, a partir del medio ambiente se estableció inicialmente en Australia en 1989, en asocio con el Eucalyptus camaldulensis y posteriormente con E. tereticornis. Con estos hallazgos se postuló que desde allí, el hongo se ha podido exportar, por medio de las semillas contaminadas, a otras regiones geográficas, incluyendo Colombia. El objetivo de éste estudio fue identificar las levaduras del género Cryptococcus asociadas con especies de Eucalyptus sp., como primera evaluación en la ecología de C. neoformans var. gattii en nuestro país. Se realizó en Santafé de Bogotá, con una población de 100 árboles ubicados al centro, nororiente, oriente y occidente de la ciudad, recolectando de cada uno de ellos flores, frutos, hojas, cortezas y detritos; el procesamiento de las muestras incluyó extracción del material con una solución salina con antibióticos, siembra en medios selectivos e identificación de las especies con base en las características morfológicas, macro y microscópicas y bioquímicas. Se aislaron 27 cepas de Cryptococcus pertenecientes a 9 especies de Cryptococcus, a partir de 21 árboles ubicados en 5 zonas diferentes de la ciudad. Se aisló C. neoformans y se identificó como C. neoformans var. neoformans serotipo A. Estos datos iniciales son importantes como primera evaluación de la asociación de Cryptococcus sp. con los Eucalyptus en nuestro país.Environmental isolation of Cryptococcus neoformans var. gattii was first made in Australia in 1989 by ELLIS. He established a specific association with the tree species Eucalyptus camaldulensis and E. tereticornis. Based on his findings, ELLIS proposed that the fungus could be exported from Australia to others regions, including Colombia, by means of infected seeds. The purpose of this study was to isolate and identify Cryptococcus sp., associated with Eucalyptus trees; this is the first ecological evaluation

  3. Antimicrobial, Physicochemical, Mechanical, and Barrier Properties of Tapioca Starch Films Incorporated with Eucalyptus Extract

    Directory of Open Access Journals (Sweden)

    M. Rojhan

    2013-11-01

    Full Text Available Starch is found in abundance in nature and it is one of the raw materials used for food packagingbecause of the low price, biodegradability, good mechanical and barrier properties. The recycling ability ofcoating materials was significantly increased by using edible films and coating in comparison to traditionalpackaging and it could be an alternative for synthetic films. In this research, the effect of eucalyptus extract(Aqueous Extract was investigated on tapioca starch films. Tapioca starch films were prepared by castingmethod with addition of eucalyptus extract and a mixture of sorbitol/glycerol (weight ratio of 3 to 1 asplasticizers. Eucalyptus extract incorporated to the tapioca starch films were dried at different concentrations(0, 15, 25, and 35 of total solid under controlled conditions. Physicochemical properties such as waterabsorption capacity (WAC, water vapor permeability (WVP and mechanical properties of the films wereevaluated. Results showed that by increasing the concentration of eucalyptus extract, tensile strength wasincreased from 20.60 to 15.68 (MPa, also elongation was increased from 19.31 to 23.57 (% at break andYoung’s modulus was decreased from 800.31 to 500.32 (MPa. Also incorporation of eucalyptus extract in thestructure of biopolymer increased permeability of water vapor of starch films. Tapioca starch filmsincorporated with eucalyptus extract exhibited excellent antimicrobial activity against E. Coli. In summary,eucalyptus extract improves functional properties of tapioca starch films and this types of films can be used infood packaging.

  4. Pretreatment of eucalyptus with recycled ionic liquids for low-cost biorefinery.

    Science.gov (United States)

    Xu, Jikun; Liu, Bingchuan; Hou, Huijie; Hu, Jingping

    2017-06-01

    It is urgent to develop recycled ionic liquids (ILs) as green solvents for sustainable biomass pretreatment. The goal of this study is to explore the availability and performance of reusing 1-allyl-3-methylimidazolium chloride ([amim]Cl) and 1-butyl-3-methylimidazolium acetate ([bmim]OAc) for pretreatment, structural evolution, and enzymatic hydrolysis of eucalyptus. Cellulose enzymatic digestibility slightly decreased with the increased number of pretreatment recycles. The hydrolysis efficiencies of eucalyptus pretreated via 4th recycled ILs were 54.3% for [amim]Cl and 72.8% for [bmim]OAc, which were 5.0 and 6.7-folds higher than that of untreated eucalyptus. Deteriorations of ILs were observed by the relatively lower sugar conversion and lignin removal from eucalyptus after 4th reuse. No appreciable changes in fundamental framework and thermal stability of [amim]Cl were observed even after successive pretreatments, whereas the anionic structure of [bmim]OAc was destroyed or replaced. This study suggested that the biomass pretreatment with recycled ILs was a potential alternative for low-cost biorefinery. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Survey of Attini (Hymenoptera, Formicidae) in Eucalyptus plantations in the region of Paraopeba, Minas Gerais, Brasil

    OpenAIRE

    Araújo, Márcio S.; Delia Lucia, Terezinha M.C.; Mayhé-Nunes, Antonio J.

    1997-01-01

    This Work was conducted in Eucalyptus stands at the Itapoã farm of the Mannesmann Fi-El Florestal Ltda. in Paraopeba, MG. The species of fungus growing-ants and leaf-cutting ants found in regrowth areas and in harvesting phase plantings were: Acromyrmex balzani Emery, 1890; Acromyrmex laticeps nigrosetosus Forel 1908; Acromyrmex subterraneus subterraneus Forel, 1893; Atta laevigala (F. Smith, 1858); Alta sexdens rubropilosa Forel, 1908; Mycocepurus goeldii Forel, 1893; Sericomyrmex sp.; Trach...

  6. USE OF SODIUM HIPOCHLORITE IN STERILIZATION OF CULTURE MEDIUM FOR MULTIPLICATION OF Eucalyptus pellita L.

    Directory of Open Access Journals (Sweden)

    Silvio Lopes Teixeira

    2009-10-01

    Full Text Available Lately it has been observed a great interest in the research area of plant tissue culture in discovering new alternatives leading to cost reduction of the plants produced in commercial laboratories, in order to turn this alternative of plant propagation more economical. A potentially promising alternative for this reduction of costs, but which has not been receiving the due attention, is the possibility of substituting the autoclaving technique to a more economical one. With this purpose, two tests were carried out, using a new protocol of medium preparation, which consisted of the chemical sterilization of all the utensils used in the preparation and packaging of the culture medium as well, associated to the addition of the sterilizing agent to the medium, in different concentrations. The objective of the first test was to observe the influence of different concentrations of NaClO added to the culture medium, on its sterilization. The second test aimed at verifying the reaction of the Eucalyptus pellita tissues to different concentrations of NaClO in the culture medium. The addition of NaClO to the culture medium, equal or higher than 0.0005% in the fist test and of 0.005% in the second one, allowed complete sterilization of the medium, without observing any damage to the Eucalyptus pellita tissues, even when they were grown on culture medium containing up to 0.009%, the maximum concentration tried. The results showed the viability of eliminating the autoclave for the sterilization of culture media.

  7. The influence of Eucalyptus plantations on the macrofauna associated with Salvinia auriculata in Southeast Brazil

    Directory of Open Access Journals (Sweden)

    M. CALLISTO

    Full Text Available The influence of Eucalyptus plantations on the structure and composition of macroinvertebrate communities associated with the aquatic fern Salvinia auriculata Aublet were investigated in a high altitude lake bordered by either secondary Atlantic forest or Eucalyptus plantations. Comparisons of the diversity of Chironomidae (Diptera, Insecta larvae in the littoral zone between these two vegetation types showed higher diversity of larvae in waters bordered by Eucalyptus. The results demonstrated that the predominance of carnivorous taxa among the macroinvertebrate fauna appears to be the major controlling factor for limiting diversity in lake areas bordered by Eucalyptus.

  8. The influence of Eucalyptus plantations on the macrofauna associated with Salvinia auriculata in Southeast Brazil

    Directory of Open Access Journals (Sweden)

    CALLISTO M.

    2002-01-01

    Full Text Available The influence of Eucalyptus plantations on the structure and composition of macroinvertebrate communities associated with the aquatic fern Salvinia auriculata Aublet were investigated in a high altitude lake bordered by either secondary Atlantic forest or Eucalyptus plantations. Comparisons of the diversity of Chironomidae (Diptera, Insecta larvae in the littoral zone between these two vegetation types showed higher diversity of larvae in waters bordered by Eucalyptus. The results demonstrated that the predominance of carnivorous taxa among the macroinvertebrate fauna appears to be the major controlling factor for limiting diversity in lake areas bordered by Eucalyptus.

  9. The floral transcriptome of Eucalyptus grandis

    CSIR Research Space (South Africa)

    Vining, KJ

    2015-10-01

    Full Text Available As a step toward functional annotation of genes required for floral initiation and development within the Eucalyptus genome, we used short read sequencing to analyze transcriptomes of floral buds from early and late developmental stages...

  10. Path analysis of the energy density of wood in eucalyptus clones.

    Science.gov (United States)

    Couto, A M; Teodoro, P E; Trugilho, P F

    2017-03-16

    Path analysis has been used for establishing selection criteria in genetic breeding programs for several crops. However, it has not been used in eucalyptus breeding programs yet. In the present study, we aimed to identify the wood technology traits that could be used as the criteria for direct and indirect selection of eucalyptus genotypes with high energy density of wood. Twenty-four eucalyptus clones were evaluated in a completely randomized design with five replications. The following traits were assessed: basic wood density, total extractives, lignin content, ash content, nitrogen content, carbon content, hydrogen content, sulfur content, oxygen content, higher calorific power, holocellulose, and energy density. After verifying the variability of all evaluated traits among the clones, a two-dimensional correlation network was used to determine the phenotypic patterns among them. The obtained coefficient of determination (0.94) presented a higher magnitude in relation to the effect of the residual variable, and it served as an excellent model for explaining the genetic effects related to the variations observed in the energy density of wood in all eucalyptus clones. However, for future studies, we recommend evaluating other traits, especially the morphological traits, because of the greater ease in their measurement. Selecting clones with high basic density is the most promising strategy for eucalyptus breeding programs that aim to increase the energy density of wood because of its high heritability and magnitude of the cause-and-effect relationship with this trait.

  11. Classification of Eucalyptus urograndis hybrids under different water availability based on biometric traits

    Directory of Open Access Journals (Sweden)

    Claudia D. Silva

    2014-08-01

    Full Text Available Aim of study: The eucalyptus grows rapidly and is well suitable to edaphic and bioclimatic conditions in several regions of of the world. The aim of this study was to assess the performance of Eucalyptus urograndis hybrids grown under different water availability conditions.Area of study: The study was performed in south-eastern of BrazilMaterial and Methods: We evaluated five commercial hybrids cultivated in pots with the substrate maintained at 65, 50, 35 and 20% maximum water retention capacity. The evaluation was based on the following characteristics: total height (cm, diameter (mm, number of leaves, leaf area (dm2, and dry weight (g plant-1 of leaf, stem + branches,   root, shoot and total and root/shoot ratio.Main results: All the characteristics evaluated were adversely affected by reduced availability of water in the substrate. The hybrids assessed performed differently in terms of biometric characteristics, irrespective of water availability. Water deficit resulted in a greater reduction in the dry weight production compared to number of leaves, diameter and height. Hybrids H2 and H5 have favorable traits for tolerating drought. The hybrid H2 shows a stronger slowdown in growth as soil moisture levels drop, although its growth rate is low, and H5 increases the root/shoot ratio but maintains growth in terms of height, even under drought conditions.Research highlights: The results obtained in our experiment show that productive hybrids sensitive to drought could also perform better under water deficit conditions, maintaining satisfactory growth despite significant drops in these characteristics.Keywords: Eucalyptus urograndis; water deficit; drought tolerance. 

  12. Thermal characterization of oil palm fiber and eucalyptus in torrefaction

    International Nuclear Information System (INIS)

    Chen, Wei-Hsin; Kuo, Po-Chih; Liu, Shih-Hsien; Wu, Wei

    2014-01-01

    Thermal behavior of biomass in torrefaction plays an important role in the operation of pretreatment. To understand the endothermic and/or exothermic characteristics of biomass in the course of torrefaction, an experimental system is conducted and two kinds of biomass (oil palm fiber and eucalyptus) are investigated. The results indicate that the thermal behavior is significantly influenced by the lignocellulosic composition in biomass and the torrefaction temperature. The thermal decomposition of hemicellulose is the dominant mechanism for oil palm fiber torrefied at 200 and 250 °C, whereas the thermal degradation of cellulose is crucial when the biomass is torrefied at 300 °C. Therefore, the heat of reaction of oil palm fiber increases with increasing torrefaction temperature. The torrefaction of eucalyptus is always endothermic, as a consequence of high cellulose contained in the biomass. It is less endothermic when the torrefaction temperature increases, presumably due to the char formation from cellulose thermal degradation and the exothermic lignin decomposition. As a whole, the values of the heat of reaction of the two samples are between −3.50 and 2.23 MJ/kg. The obtained results have provided a useful insight into the control of torrefaction operation and the design of torrefaction reactor. - Highlights: • Thermal behavior of oil palm fiber and eucalyptus in torrefaction is studied. • Thermal characteristic of biomass in torrefaction depends on lignocellulosic composition. • Heat of reaction of oil palm fiber increases with increasing torrefaction temperature. • Eucalyptus torrefaction is always endothermic because of high cellulose contained. • Torrefaction of eucalyptus is less endothermic when the torrefaction temperature increases

  13. Encapsulated eucalyptus oil in ionically cross-linked alginate microcapsules and its controlled release.

    Science.gov (United States)

    Noppakundilograt, Supaporn; Piboon, Phianghathai; Graisuwan, Wilaiporn; Nuisin, Roongkan; Kiatkamjornwong, Suda

    2015-10-20

    Sodium alginate microcapsules containing eucalyptus oil were prepared by oil-in-water emulsification via Shirasu porous glass (SPG) membrane and cross-linked by calcium chloride (CaCl2). SPG membrane pore size of 5.2μm was used to control the size of eucalyptus oil microdroplets. Effects of sodium alginate, having a mannuronic acid/guluronic acid (M/G) ratio of 1.13, eucalyptus oil and CaCl2 amounts on microdroplet sizes and size distribution were elucidated. Increasing sodium alginate amounts from 0.1 to 0.5% (wv(-1)) sodium alginate, the average droplets size increased from 42.2±2.0 to 48.5±0.6μm, with CVs of 16.5±2.2 and 30.2±4.5%, respectively. CaCl2 successfully gave narrower size distribution of cross-linked eucalyptus oil microcapsules. The optimum conditions for preparing the microcapsules, oil loading efficiency, and controlled release of the encapsulated eucalyptus oil from the microcapsules as a function of time at 40°C were investigated. Release model for the oil from microcapsules fitted Ritger-Peppas model with non-Fickian transport mechanism. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. LONGITUDINAL RESIDUAL AND TANGENTIAL STRAIN (LRS and LRT IN SIX Eucalyptus spp. CLONES

    Directory of Open Access Journals (Sweden)

    Paulo Fernando Trugilho

    2006-09-01

    Full Text Available The species of Eucalyptus genus present high levels of growth stress. These stresses are mechanical efforts generated during the tree growth to help maintaining the balance of the cup, in response to environmental (light, wind and inclination of the land and silvicultural agents (pruning, thinning and planting density. The growth stresses are responsible for the cracks of tops, in logs and boards, and for the warp after the breaking down. This research evaluated the level of growth stress, measured by the longitudinal residual and tangential strain (DRL and DRT, around the circumference of the trunks of alive trees of six clones of Eucalyptus spp., at the age of 10.5 years, and verified the effect of the planting parcel. The clones belong to VMM-AGRO, and they are coming from a clonal test area implanted in the Bom Sucesso farm, located in Vazante-MG. For evaluating the experiment, the model adopted was the completely randomized one disposed in factorial outline with two factors (clone and portion in three repetitions. The results indicated that the average LRS was 0.093 mm and that the average LRT was 0.025 mm. It was verified that, for LRS, the clone effects and planting parcel were significant, while the interaction effect was not significant. For LRT the parcel and interaction effect were significant, while clone effect was not significant. Clones 44, 58 and 47 presented the smallest levels and better distributions of LRS, while, the clones 27, 44 and 58 presented the highest LRS levels. The clones 44 and 58 presented the best distribution and the smallest level of growth stress and may be considered potentially apt for producing sawn wood or solid wood.

  15. Behavior of Eucalyptus urophylla and Eucalyptus citriodora Seedlings Grown in Soil Contaminated by Arsenate

    Directory of Open Access Journals (Sweden)

    Roseli Freire Melo

    2016-01-01

    Full Text Available ABSTRACT Persistent areas of tailings and deposits from coal and gold mining may present high levels of arsenic (As, mainly in the arsenate form, endangering the environment and human health. The establishment of vegetation cover is a key step to reclaiming these environments. Thus, this study aimed to evaluate the potential of Eucalyptus urophylla and E. citriodora seedlings for use in phytoremediation programs of arsenate-contaminated areas. Soil samples were incubated at increasing rates (0, 50, 100, 200 and 400 mg dm-3 of arsenic (arsenate form, using Na2HAsO4 for 15 days. The seedlings were produced in a substrate (vermiculite + sawdust and were transplanted to the pots with soil three months after seed germination. The values of plant height and diameter were taken during transplanting and 30, 60 and 90 days after transplanting. In the last evaluation, the total leaf area and biomass of shoots and roots were also determined. The values of available As in soil which caused a 50 % dry matter reduction (TS50%, the As translocation index (TI from the roots to the shoot of the plants, and its bioconcentration factor (BF were also calculated. Higher levels of arsenate in the soil significantly reduced the dry matter production of roots and shoots and the height of both species, most notably in E. urophylla plants. The highest levels of As were found in the root, with higher values for E. citriodora (ranging from 253.86 to 400 mg dm-3. The TI and BF were also reduced with As doses, but the values found in E. citriodora were significantly higher than in E. urophylla. E. citriodora plants presented a higher capacity to tolerate As and translocate it to the shoot than E. urophylla. Although these species cannot be considered as hyperaccumulators of As, E. citriodora presented the potential to be used in phytoremediation programs in arsenate-contaminated areas due to the long-term growth period of this species.

  16. Eucalyptus biodiesel as an alternative to diesel fuel: preparation and tests on DI diesel engine.

    Science.gov (United States)

    Tarabet, Lyes; Loubar, Khaled; Lounici, Mohand Said; Hanchi, Samir; Tazerout, Mohand

    2012-01-01

    Nowadays, the increasing oil consumption throughout the world induces crucial economical, security, and environmental problems. As a result, intensive researches are undertaken to find appropriate substitution to fossil fuels. In view of the large amount of eucalyptus trees present in arid areas, we focus in this study on the investigation of using eucalyptus biodiesel as fuel in diesel engine. Eucalyptus oil is converted by transesterification into biodiesel. Eucalyptus biodiesel characterization shows that the physicochemical properties are comparable to those of diesel fuel. In the second phase, a single cylinder air-cooled, DI diesel engine was used to test neat eucalyptus biodiesel and its blends with diesel fuel in various ratios (75, 50, and 25 by v%) at several engine loads. The engine combustion parameters such as peak pressure, rate of pressure rise, and heat release rate are determined. Performances and exhaust emissions are also evaluated at all operating conditions. Results show that neat eucalyptus biodiesel and its blends present significant improvements of carbon monoxide, unburned hydrocarbon, and particulates emissions especially at high loads with equivalent performances to those of diesel fuel. However, the NOx emissions are slightly increased when the biodiesel content is increased in the blend.

  17. Genome-Wide Characterization and Expression Profiling of the AUXIN RESPONSE FACTOR (ARF) Gene Family in Eucalyptus grandis

    Science.gov (United States)

    Yu, Hong; Soler, Marçal; Mila, Isabelle; San Clemente, Hélène; Savelli, Bruno; Dunand, Christophe; Paiva, Jorge A. P.; Myburg, Alexander A.; Bouzayen, Mondher; Grima-Pettenati, Jacqueline; Cassan-Wang, Hua

    2014-01-01

    Auxin is a central hormone involved in a wide range of developmental processes including the specification of vascular stem cells. Auxin Response Factors (ARF) are important actors of the auxin signalling pathway, regulating the transcription of auxin-responsive genes through direct binding to their promoters. The recent availability of the Eucalyptus grandis genome sequence allowed us to examine the characteristics and evolutionary history of this gene family in a woody plant of high economic importance. With 17 members, the E. grandis ARF gene family is slightly contracted, as compared to those of most angiosperms studied hitherto, lacking traces of duplication events. In silico analysis of alternative transcripts and gene truncation suggested that these two mechanisms were preeminent in shaping the functional diversity of the ARF family in Eucalyptus. Comparative phylogenetic analyses with genomes of other taxonomic lineages revealed the presence of a new ARF clade found preferentially in woody and/or perennial plants. High-throughput expression profiling among different organs and tissues and in response to environmental cues highlighted genes expressed in vascular cambium and/or developing xylem, responding dynamically to various environmental stimuli. Finally, this study allowed identification of three ARF candidates potentially involved in the auxin-regulated transcriptional program underlying wood formation. PMID:25269088

  18. Genome-wide characterization and expression profiling of the AUXIN RESPONSE FACTOR (ARF gene family in Eucalyptus grandis.

    Directory of Open Access Journals (Sweden)

    Hong Yu

    Full Text Available Auxin is a central hormone involved in a wide range of developmental processes including the specification of vascular stem cells. Auxin Response Factors (ARF are important actors of the auxin signalling pathway, regulating the transcription of auxin-responsive genes through direct binding to their promoters. The recent availability of the Eucalyptus grandis genome sequence allowed us to examine the characteristics and evolutionary history of this gene family in a woody plant of high economic importance. With 17 members, the E. grandis ARF gene family is slightly contracted, as compared to those of most angiosperms studied hitherto, lacking traces of duplication events. In silico analysis of alternative transcripts and gene truncation suggested that these two mechanisms were preeminent in shaping the functional diversity of the ARF family in Eucalyptus. Comparative phylogenetic analyses with genomes of other taxonomic lineages revealed the presence of a new ARF clade found preferentially in woody and/or perennial plants. High-throughput expression profiling among different organs and tissues and in response to environmental cues highlighted genes expressed in vascular cambium and/or developing xylem, responding dynamically to various environmental stimuli. Finally, this study allowed identification of three ARF candidates potentially involved in the auxin-regulated transcriptional program underlying wood formation.

  19. Speciation and distribution of Botryosphaeria spp. on native and introduced Eucalyptus trees in Australia and South Africa

    NARCIS (Netherlands)

    Slippers, B.; Fourie, G.; Crous, P.W.; Coutinho, T.A.; Wingfield, B.D.; Carnegie, A.J.; Wingfield, M.J.

    2004-01-01

    Botryosphaeria spp. are important canker and die-back pathogens that affect Eucalyptus spp. They also occur endophytically in Eucalyptus leaves and stems. For the purpose of this study, Botryosphaeria strains were isolated from diseased and symptomless Eucalyptus material from Australia and South

  20. Mortality of the defoliator Euselasia eucerus (Lepidoptera: Riodinidae by biotic factors in an Eucalyptus urophylla plantation in Minas Gerais State, Brazil

    Directory of Open Access Journals (Sweden)

    José C. Zanuncio

    2009-03-01

    Full Text Available Euselasia eucerus (Hewitson, 1872 (Lepidoptera: Riodinidae is a Brazilian native species commonly found in Eucalyptus plantations. Biotic mortality factors of this defoliator were studied in a Eucalyptus urophylla plantation in Minas Gerais State, Brazil aiming to identify natural enemies and their impact on this insect. Euselasia eucerus had biotic mortality factors during all development stages. The most important were Trichogramma maxacalii Voegelé and Pointel, 1980 (Hymenoptera: Trichogrammatidae during egg stage (48.9%, a tachinid fly (Diptera: Tachinidae during larval stages (10% and Itoplectis sp. (Hymenoptera: Ichneumonidae during pupal stage (52.2%. The parasitism rate was higher in the basal part of the plant canopy (37.8%.Euselasia eucerus (Hewitson, 1872 (Lepidoptera: Riodinidae é uma espécie brasileira nativa, comumente encontrada em plantios de Eucalyptus. Um estudo da mortalidade por fatores bióticos desse desfolhador foi feito em um plantio de Eucalyptus urophylla no Estado de Minas Gerais, Brasil, com o objetivo de identificar os inimigos naturais e seu impacto sobre esse lepidóptero. Euselasia eucerus possui fatores bióticos de mortalidade durante todas as suas fases de desenvolvimento. Os mais importantes foram Trichogramma maxacalii Voegelé e Pointel, 1980 (Hymenoptera: Trichogrammatidae durante a fase de ovo (48,9%, um Diptera: Tachinidae durante a fase de larva (10% e Itoplectis sp. (Hymenoptera: Ichneumonidae durante a fase pupal (52,2%. A taxa de parasitismo foi mais elevada na parte basal de plantas de eucalipto (37,8%.