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Sample records for eucalyptus globulus cdna

  1. Eucalyptus

    Science.gov (United States)

    Blue Gum, Blue Mallee, Blue Mallee Oil, Eucalipto, Eucalypti Folium, Eucalyptol, Eucalyptol Oil, Eucalyptus blatter, Eucalyptus bicostata, Eucalyptus Essential Oil, Eucalyptus Oil, Eucalyptus fructicetorum, Eucalyptus globulus, Eucalyptus Leaf, Eucalyptus odorata, Eucalyptus ...

  2. A NEW SPECIES OF INVASIVE GALL WASP (HYMENOPTERA: EULOPHIDAE: TETRASTICHINAE) ON BLUE GUM (EUCALYPTUS GLOBULUS) IN CALIFORNIA

    Science.gov (United States)

    The blue gum gall wasp, Selitrichodes globulus La Salle & Gates (Hymenoptera: Eulophidae: Tetrastichinae), is described as an invasive gall inducer on blue gum, Eucalyptus globulus (Myrtaceae), in California....

  3. Nitrogen-Fixing Bacteria in Eucalyptus globulus Plantations

    Science.gov (United States)

    da Silva, Marliane de Cássia Soares; Paula, Thiago de Almeida; Moreira, Bruno Coutinho; Carolino, Manuela; Cruz, Cristina; Bazzolli, Denise Mara Soares; Silva, Cynthia Canedo; Kasuya, Maria Catarina Megumi

    2014-01-01

    Eucalypt cultivation is an important economic activity worldwide. In Portugal, Eucalyptus globulus plantations account for one-third of the total forested area. The nutritional requirements of this crop have been well studied, and nitrogen (N) is one of the most important elements required for vegetal growth. N dynamics in soils are influenced by microorganisms, such as diazotrophic bacteria (DB) that are responsible for biological nitrogen fixation (BNF), so the aim of this study was to evaluate and identity the main groups of DB in E. globulus plantations. Samples of soil and root systems were collected in winter and summer from three different Portuguese regions (Penafiel, Gavião and Odemira). We observed that DB communities were affected by season, N fertilization and moisture. Furthermore Bradyrhizobium and Burkholderia were the most prevalent genera in these three regions. This is the first study describing the dynamic of these bacteria in E. globulus plantations, and these data will likely contribute to a better understanding of the nutritional requirements of eucalypt cultivation and associated organic matter turnover. PMID:25340502

  4. Nitrogen-fixing bacteria in Eucalyptus globulus plantations.

    Science.gov (United States)

    da Silva, Marliane de Cássia Soares; Paula, Thiago de Almeida; Moreira, Bruno Coutinho; Carolino, Manuela; Cruz, Cristina; Bazzolli, Denise Mara Soares; Silva, Cynthia Canedo; Kasuya, Maria Catarina Megumi

    2014-01-01

    Eucalypt cultivation is an important economic activity worldwide. In Portugal, Eucalyptus globulus plantations account for one-third of the total forested area. The nutritional requirements of this crop have been well studied, and nitrogen (N) is one of the most important elements required for vegetal growth. N dynamics in soils are influenced by microorganisms, such as diazotrophic bacteria (DB) that are responsible for biological nitrogen fixation (BNF), so the aim of this study was to evaluate and identity the main groups of DB in E. globulus plantations. Samples of soil and root systems were collected in winter and summer from three different Portuguese regions (Penafiel, Gavião and Odemira). We observed that DB communities were affected by season, N fertilization and moisture. Furthermore Bradyrhizobium and Burkholderia were the most prevalent genera in these three regions. This is the first study describing the dynamic of these bacteria in E. globulus plantations, and these data will likely contribute to a better understanding of the nutritional requirements of eucalypt cultivation and associated organic matter turnover.

  5. Nitrogen-fixing bacteria in Eucalyptus globulus plantations.

    Directory of Open Access Journals (Sweden)

    Marliane de Cássia Soares da Silva

    Full Text Available Eucalypt cultivation is an important economic activity worldwide. In Portugal, Eucalyptus globulus plantations account for one-third of the total forested area. The nutritional requirements of this crop have been well studied, and nitrogen (N is one of the most important elements required for vegetal growth. N dynamics in soils are influenced by microorganisms, such as diazotrophic bacteria (DB that are responsible for biological nitrogen fixation (BNF, so the aim of this study was to evaluate and identity the main groups of DB in E. globulus plantations. Samples of soil and root systems were collected in winter and summer from three different Portuguese regions (Penafiel, Gavião and Odemira. We observed that DB communities were affected by season, N fertilization and moisture. Furthermore Bradyrhizobium and Burkholderia were the most prevalent genera in these three regions. This is the first study describing the dynamic of these bacteria in E. globulus plantations, and these data will likely contribute to a better understanding of the nutritional requirements of eucalypt cultivation and associated organic matter turnover.

  6. Energy evaluation of the Eucalyptus globulus and the Eucalyptus nitens in the north of Spain (Cantabria)

    International Nuclear Information System (INIS)

    Perez, S.; Renedo, C.J.; Ortiz, A.; Manana, M.; Silio, D.

    2006-01-01

    This work studied the potential use of the waste from Eucalyptus globulus and Eucalyptus nitens as energy crops, evaluating young and adult stages of both in all four seasons of the year with different moisture contents. The study was carried out made in Cantabria (North coast of Spain), located at latitude 43 o 28'N, and longitude 3 o 48'W. In this region, 29,513ha are dedicated to the growth of Eucalyptus, with about 80% E. globulus, and 20% E. nitens. Six different plantations have been analyzed and their bioclimatic diagrams determined. After the collection of samples the potential energy of every sample was obtained, they were weighed, analyzed and burned, giving a mean net calorific value of 17,384 and 17,927kJ/kg in the adult stage of E. globulus and E. nitens, respectively. The results for the young stage of both species were 17,708 and 18,670kJ/kg. Moisture content in the samples has a great influence on power production. Finally, the economic and environmental consequences of these crop species for the region of Cantabria were analyzed. (author)

  7. Yield models for Eucalyptus globulus fuelwood plantations in Ethiopia

    Energy Technology Data Exchange (ETDEWEB)

    Pukkala, T.; Pohjonen, V. (Joensuu Univ. (FI). Faculty of Forestry)

    1990-01-01

    Based on 53 tree analyses and 105 sample plots of Eucalyptus globulus, models for volume and biomass at single tree and stand levels were developed. The possible growing sites were divided into four site classes. In seedling stands, the site class I corresponds to yield class 44 m{sup 3} ha{sup -1} year{sup -1}, in coppice stands to yield class 46 m{sup 3} ha{sup -1} year{sup -1}. The site class IV corresponds in seedling stand to yield class 9 m{sup 3} ha{sup -1} year{sup -1}, in coppice stands to yield class 13 m{sup 3} ha{sup -1} year{sup -1}. The maximum mean annual increment was reached in seedling stands at the age of 18-19 years, in coppice stands at the age of 14 years. (author).

  8. Uso de antraquinona en cocción kraft de Eucalyptus globulus y Eucalyptus nitens

    OpenAIRE

    Moreira, Jorge Felipe Quintana

    2011-01-01

    En la presente disertación se verificó de manera experimental el efecto de la adición de Antraquinona al proceso de cocción batch convencional para una mezcla industrial de Eucalyptus globulus E. nitens.; en lo que respecta a su capacidad de aumentar la velocidad de la reacción de deslignificación y proporcionar estabilidad a las hemicelulosas y celulosas de la fibra. Lo anterior se logró con experiencias de laboratorio fijando las condiciones de cocción de acuerdo a la operación de los dig...

  9. Phytoremediation efficiency OF CD by Eucalyptus globulus transplanted from polluted and unpolluted sites.

    Science.gov (United States)

    Luo, Jie; Qi, Shihua; Peng, Li; Wang, Jinji

    2016-01-01

    The capacity of plants to uptake heavy metals from contaminated soils has shown great phytoremediation potential. The development, resistibility and Cd extraction of Eucalyptus globulus individuals from metalliferous and clean sites in different years were analyzed under a specific environment. Eucalyptus globulus planted in Guiyu for phytoremediation or cultivated in an uncontaminated, natural environment for economic purposes were transplanted to Yuecheng town, which, in recent years, has been involved in the e-waste dismantling and recycling business, to compare the phytoremediation efficiency of Eucalyptus globulus trees grown in different environments. Trees cultivated in polluted areas can remove far more Cd and Hg from the contaminated soil than the individuals from clean soils because metalliferous Eucalyptus globulus can produce more biomass and uptake more heavy metals than nonmetalliferous plants per year. As polluted environments negatively affect the growth of plants, we speculated that the phytoremediation efficiency of metalliferous Eucalyptus globulus should decrease over time and that nonmetalliferous trees should adapt to the local environment.

  10. Mycosphaerella and Teratosphaeria species associated with leaf diseases on Eucalyptus globulus in southern Brazil

    NARCIS (Netherlands)

    Teodoro, M.G.; Ferreira, M.A.; Guimarães, L.M.S.; Mafia, R.G.; Groenewald, J.Z.; Crous, P.W.; Alfenas, A.C.

    2012-01-01

    Leaf blight and defoliation caused by Teratosphaeria species is one of the most important leaf diseases of Eucalyptus globulus. Due to the importance of this tree species for the production of pulp and paper, and recent reports of severe leaf disease symptoms in Brazil, the present study was

  11. Comparison of Fractionation Techniques of CO2 Extracts from Eucalyptus Globulus - Composition and Insecticidal Activity

    Czech Academy of Sciences Publication Activity Database

    Topiař, Martin; Sajfrtová, Marie; Pavela, R.; Machalová, Zdeňka

    2015-01-01

    Roč. 97, FEB 2015 (2015), s. 202-210 ISSN 0896-8446 R&D Projects: GA TA ČR TA01010578 Institutional support: RVO:67985858 Keywords : supercritical fractinacion * eucalyptus globulus * insecticidal activity Subject RIV: CI - Industrial Chemistry , Chemical Engineering Impact factor: 2.579, year: 2015

  12. Managing for water-use efficient wood production in Eucalyptus globulus plantations

    Science.gov (United States)

    Donald A. White; John F. McGrath; Michael G. Ryan; Michael Battaglia; Daniel S. Mendham; Joe Kinal; Geoffrey M. Downes; D. Stuart Crombie; Mark E. Hunt

    2014-01-01

    This paper tests the hypothesis that thinning and nitrogen fertiliser can increase the mass of wood produced per volume of water used (evapotranspiration) by plantations of Eucalyptus globulus. We have called this plantation water productivity (PWPWOOD) and argue that, for a given genotype, this term integrates the effects of management, site and climate on both...

  13. Rethinking Eucalyptus globulus Labill. Based Land Use Systems in Smallholder Farmers Livelihoods: A Case of Kolobo Watershed, West Shewa, Ethiopia

    Directory of Open Access Journals (Sweden)

    Feyisa Dadi

    2018-03-01

    Full Text Available Despite their restriction, smallholder farmers have been continuing growing Eucalyptus globulus in the cultivated land in the central highland of Ethiopia. Literature has shown controversial issues against E. globulus. Therefore, the objective of the study was to investigate the compatibility of E. globulus in the smallholder farmers’ land use system. Soil samples were collected from five different land uses and analysed for selected physical and chemical properties. The socioeconomic contribution of E. globulus was collected through household surveys from 110 households. Analysis of soil showed that organic carbon (OC, total nitrogen (TN and cation exchange capacity (CEC were significantly higher (P<0.05 under E. globulus compared to the cultivated land. The survey results also showed that the largest proportion (58% of households was interested in growing E. globulus because of its multiple uses. About 83% of households responded that E. globulus help them to attain food security through increasing the purchasing power of smallholder farmers to buy agricultural inputs and food. This study has substantiated the role of E. globulus in the land use system of smallholder farmers. Most of the soil fertility indicators were better under E. globulus. The present finding reveals that E. globulus degrade the soil seemingly difficult to generalise. Growing E. globulus must be promoted under appealing land use to enhance smallholder farmers’ livelihoods. Removing E. globulus from the land use system may jeopardise the food security situation of many households.

  14. Litter production and decomposition in Eucalyptus urophylla x Eucalyptus globulus maidenii stand

    Directory of Open Access Journals (Sweden)

    Mauro Valdir Schumacher

    2013-09-01

    Full Text Available he sustainable wood production in commercial plantations requires knowledge of the nutrient cycling process, which also involves the production and decomposition of litter. This study verified the influence of climatic variables on litter production and t evaluated the rate of leaf litter decomposition in a stand of Eucalyptus urophylla x E. globulus maidenii. There were installed 4 plots of 20 m x 20 m, in each plot four litter traps to collect leaves were placed, thin branches and miscellaneous, beside this, each plot received 3 areas for coarse branches collection. The litter collected was used to calculate the deposition and the correlation between climate variables and deposition. The climatic variables used, on a monthly basis, were average temperature, average maximum temperature, average minimum temperature, rainfall, relative humidity, average wind speed, average solar radiation and average evapotranspiration, both supplied by an experimental station. For evaluation of the litter decomposition rate, four square samples of 0.25 m side in each plot were randomly collected and used for determining the decay coefficient (K, half life (t0,5 and decomposition time of 95% of litter (t0,95 . The monthly litter production was weakly correlated with climatic variables and the annual production was 7.4 Mg ha-1, with leaves as the major fraction (60%. The litter decomposition rate was considered slow.

  15. Mycosphaerella and Teratosphaeria species associated with Mycosphaerella Leaf Disease on Eucalyptus globulus in Portugal

    Energy Technology Data Exchange (ETDEWEB)

    Silva, M. C.; Machado, H. N.; Neves, L.; Araujo, C.; Phillips, J. L.

    2012-11-01

    Plantations of Eucalyptus globulus represent the main source of wood for the pulp and paper industry in Portugal and are affected by the complex of Mycosphaerella and Teratosphaeria species (Mycosphaerella leaf disease), which is an important foliage disease worldwide. This disease affect mainly young trees with juvenile-phase foliage, causing premature defoliation, decreased growth and wood production. Species of Mycosphaerella sensu lato reported on eucalypts in Portugal are M. communis, M. heimii, M. lateralis, M. madeirae, M. marksii M. walkeri, T. africana, T. molleriana, T. nubilosa and T. parva. In order to complete the survey, symptomatic leaves were collected from Eucalyptus globulus plantations. Morphological and molecular characterization was used to give an indication of the species occurrence and most frequent species (T. nubilosa) and the composition of the MLD complex that did not change after the latest review. (Author) 28 refs.

  16. Organic matter production in an age series of Eucalyptus globulus plantations in Tamil Nadu

    Energy Technology Data Exchange (ETDEWEB)

    Negi, J D.S.; Bora, N K.S.; Tandon, V N; Thapliyal, H D

    1984-08-01

    The distribution of organic matter in an age series of Eucalyptus globulus plantations in Tamil Nadu is discussed. The total biomass ranges from 38 tonnes (5 years) to 220 tonnes (16 years) per ha with 85 to 88 percent being contributed by the aboveground parts and 15 to 12 percent by the roots and the average annual production of non-photosynthetic components is at its peak (19 tonnes/ha) at the age of 7 years. 17 references, 4 tables.

  17. Can Acceptable Pulp be Obtained from Eucalyptus globulus Wood Chips after Hemicellulose Extraction?

    OpenAIRE

    Francisco López; M. Trinidad García; Vicente Mena; J. Mauricio Loaiza; Minerva A. M. Zamudio; Juan C. García

    2014-01-01

    This study investigates the operating conditions used in the soda-anthraquinone pulping of Eucalyptus globulus wood after autohydrolysis pretreatment on the yield, kappa number, and brightness of the resulting unbleached pulp. Moreover, strength-related properties of the resulting handsheets was examined to identify the best pulping conditions and compare the outcome with that of a conventional soda-anthraquinone pulping process. The paper strength properties of the pulp were similar to or be...

  18. Assessing nitrogen fixation in mixed- and single-species plantations of Eucalyptus globulus and Acacia mearnsii.

    Science.gov (United States)

    Forrester, David I; Schortemeyer, Marcus; Stock, William D; Bauhus, Jürgen; Khanna, Partap K; Cowie, Annette L

    2007-09-01

    Mixtures of Eucalyptus globulus Labill. and Acacia mearnsii de Wildeman are twice as productive as E. globulus monocultures growing on the same site in East Gippsland, Victoria, Australia, possibly because of increased nitrogen (N) availability owing to N(2) fixation by A. mearnsii. To investigate whether N(2) fixation by A. mearnsii could account for the mixed-species growth responses, we assessed N(2) fixation by the accretion method and the (15)N natural abundance method. Nitrogen gained by E. globulus and A. mearnsii mixtures and monocultures was calculated by the accretion method with plant and soil samples collected 10 years after plantation establishment. Nitrogen in biomass and soil confirmed that A. mearnsii influenced N dynamics. Assuming that the differences in soil, forest floor litter and biomass N of plots containing A. mearnsii compared with E. globulus monocultures were due to N(2) fixation, the 10-year annual mean rates of N(2) fixation were 38 and 86 kg ha(-1) year(-1) in 1:1 mixtures and A. mearnsii monocultures, respectively. Nitrogen fixation by A. mearnsii could not be quantified on the basis of the natural abundance of (15)N because such factors as mycorrhization type and fractionation of N isotopes during N cycling within the plant confounded the effect of the N source on the N isotopic signature of plants. This study shows that A. mearnsii fixed significant quantities of N(2) when mixed with E. globulus. A decline in delta(15)N values of E. globulus and A. mearnsii with time, from 2 to 10 years, is further evidence that N(2) was fixed and cycled through the stands. The increased aboveground biomass production of E. globulus trees in mixtures when compared with monocultures can be attributed to increases in N availability.

  19. Acaricidal and repellent activities of essential oil of Eucalyptus globulus against Dermanyssus gallinae (Acari: Mesostigmata

    Directory of Open Access Journals (Sweden)

    Dehghani-Samani Amir

    2015-07-01

    Full Text Available Introduction: By considering an increase in drug resistance against red mites, finding the nonchemical herbal acaricide against Dermanyssus gallinae (De Geer (Acari: Mesostigmata is necessary to kill them and to reduce the chemical resistance against chemical acaricides in this specie. Dermanyssus gallinae is a potential vector of the causal agent of several viral diseases such as Equine encephalitis and St. Louis encephalitis. It can be a vector of bacteria such as Salmonella spp., Mycobacterium spp. and Erysipelothrix rhusiopathiae. It is also known to cause itching dermatosis in humans. In this study acaricidal and repellent activities of essential oil of Eucalyptus globulus against Dermanyssus gallinae were studied. Methods: After extracting the essential oil, different concentrations of the plant extract were prepared. Then, acaricidal effect of different concentrations was tested on poultry red mite, Dermanyssus gallinae, by dropping 3-4 drops of essential oil on mites. Repellent activity of essential oil was tested by Y-tube olfactometer bioassay. After the test, total number of killed and repellent mites reported. Results: Concentration of 1:2 or 50% had more acaricidal effect on mites. Also essential oil of Eucalyptus globulus had repellent activity against red mites. Conclusion: This study showed that essential oil of Eucalyptus globulus had acaricidal and repellent activities against red mites. Hence it might be used as a herbal acaricide against it to kill and to reduce the chemical resistance in this specie.

  20. Eucalyptus globulus extract protects against UVB-induced photoaging by enhancing collagen synthesis via regulation of TGF-β/Smad signals and attenuation of AP-1.

    Science.gov (United States)

    Park, Bom; Hwang, Eunson; Seo, Seul A; Cho, Jin-Gyeong; Yang, Jung-Eun; Yi, Tae-Hoo

    2018-01-01

    UV irradiation triggers the overproduction of matrix metalloproteinases and collagen degradation, which in turn causes increased pigmentation, dryness, and deep wrinkling of the skin. These chronic symptoms are collectively referred to as photoaging. Eucalyptus globulus is an evergreen tree that is widely used in cosmetics because of its antimicrobial activity. In this study, we investigated the protective effect of 50% ethanol extracts of Eucalyptus globulus on UV-induced photoaging in vitro and in vivo. Normal human dermal fibroblasts were treated with Eucalyptus globulus at concentrations ranging from 1 to 100 μg/mL after UVB or non-UVB irradiation. We found that Eucalyptus globulus suppressed the expression of MMPs and IL-6, but increased the expression of TGF-β1 and procollagen type 1. In addition, Eucalyptus globulus inhibited activation of the AP-1 transcription factor, an inducer of MMPs. Eucalyptus globulus was also found to regulate TGF-β/Smad signaling by reversing the activity of negative Smad regulators. Lastly, in vivo studies showed that topical application of Eucalyptus globulus on UVB-irradiated hairless mice reduced wrinkle formation and dryness by down-regulating MMP-1 and up-regulating expression of elastin, TGF-β1, and procollagen type 1. Taken together, these data suggest that Eucalyptus globulus may be a useful agent in cosmetic products. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. Development of an acetic acid tolerant Spathaspora passalidarum strain through evolutionary engineering with resistance to inhibitors compounds of autohydrolysate of Eucalyptus globulus

    DEFF Research Database (Denmark)

    Morales, Paulina; Gentina, Juan Carlos; Aroca, German

    2017-01-01

    -fold higher than those obtained with the native strain, respectively. Inhibitors composition present inEucalyptus globulus autohydrolysate were (g L−1): acetic acid, 4.7; furfural, 1.0; HMF, 0.36; formic acid, 0.6;syringaldehyde, 0.14 and vanillin, 0.017. When Eucalyptus globulus autohydrolysate...

  2. A model system to study the lignification process in Eucalyptus globulus.

    Science.gov (United States)

    Araújo, Pedro; Cesarino, Igor; Mayer, Juliana Lischka Sampaio; Ferrari, Ilse Fernanda; Kiyota, Eduardo; Sawaya, Alexandra Christine Helena Frankland; Paes Leme, Adriana Franco; Mazzafera, Paulo

    2014-09-01

    Recalcitrance of plant biomass is closely related to the presence of the phenolic heteropolymer lignin in secondary cell walls, which has a negative effect on forage digestibility, biomass-to-biofuels conversion and chemical pulping. The genus Eucalyptus is the main source of wood for pulp and paper industry. However, when compared to model plants such as Arabidopsis thaliana and poplar, relatively little is known about lignin biosynthesis in Eucalyptus and only a few genes were functionally characterized. An efficient, fast and inexpensive in vitro system was developed to study lignification in Eucalyptus globulus and to evaluate the potential role of candidate genes in this biological process. Seedlings were grown in four different conditions, in the presence or absence of light and with or without sucrose in the growth medium, and several aspects of lignin metabolism were evaluated. Our results showed that light and, to a lesser extent, sucrose induced lignin biosynthesis, which was followed by changes in S/G ratio, lignin oligomers accumulation and gene expression. In addition, higher total peroxidase activity and differential isoperoxidase profile were observed when seedlings were grown in the presence of light and sucrose. Peptide sequencing allowed the identification of differentially expressed peroxidases, which can be considered potential candidate class III peroxidases involved in lignin polymerization in E. globulus. © 2014 Scandinavian Plant Physiology Society.

  3. Unravelling the bioherbicide potential of Eucalyptus globulus Labill: Biochemistry and effects of its aqueous extract.

    Science.gov (United States)

    Puig, Carolina G; Reigosa, Manuel J; Valentão, Patrícia; Andrade, Paula B; Pedrol, Nuria

    2018-01-01

    In the worldwide search for new strategies in sustainable weed management, the use of plant species able to produce and release phytotoxic compounds into the environment could be an effective alternative to synthetic herbicides. Eucalyptus globulus Labill. is known to be a source of biologically active compounds responsible for its phytotoxic and allelopathic properties. Our previous results demonstrated the bioherbicide potential of eucalyptus leaves incorporated into the soil as a green manure, probably through the release of phytotoxins into the soil solution. Thus, the aims of this study were to understand the phytotoxicity of the eucalyptus leaves aqueous extract applied in pre- and post-emergence, and to identify and quantify its potentially phytotoxic water-soluble compounds. The effects were tested on the germination and early growth of the model target species Lactuca sativa and Agrostis stolonifera, and on physiological parameters of L. sativa adult plants after watering or spraying application. Dose-response curves and ED50 and ED80 values for eucalyptus aqueous extracts revealed pre-emergence inhibitory effects on both target species, effects being comparable to the herbicide metolachlor. While spraying treatment reduced the aerial and root biomass and increased the dry weight/fresh weight ratio of lettuce adult plants, watering application reduced protein contents and chlorophyll concentrations with respect to control, reflecting different modes of action depending on the site of phytotoxin entry. Via HPLC analyses, a total of 8 phenolic compounds (chlorogenic, two ρ-coumaric derivatives, ellagic, hyperoside, rutin, quercitrin, and kaempferol 3-O-glucoside) and other 5 low weight organic acids (citric, malic, shikimic, succinic and fumaric acids) were obtained from aqueous extract, the latter being identified for the first time in E. globulus. Despite some phytotoxic effects were found on lettuce adult plants, the use of eucalyptus aqueous extract

  4. Unravelling the bioherbicide potential of Eucalyptus globulus Labill: Biochemistry and effects of its aqueous extract.

    Directory of Open Access Journals (Sweden)

    Carolina G Puig

    Full Text Available In the worldwide search for new strategies in sustainable weed management, the use of plant species able to produce and release phytotoxic compounds into the environment could be an effective alternative to synthetic herbicides. Eucalyptus globulus Labill. is known to be a source of biologically active compounds responsible for its phytotoxic and allelopathic properties. Our previous results demonstrated the bioherbicide potential of eucalyptus leaves incorporated into the soil as a green manure, probably through the release of phytotoxins into the soil solution. Thus, the aims of this study were to understand the phytotoxicity of the eucalyptus leaves aqueous extract applied in pre- and post-emergence, and to identify and quantify its potentially phytotoxic water-soluble compounds. The effects were tested on the germination and early growth of the model target species Lactuca sativa and Agrostis stolonifera, and on physiological parameters of L. sativa adult plants after watering or spraying application. Dose-response curves and ED50 and ED80 values for eucalyptus aqueous extracts revealed pre-emergence inhibitory effects on both target species, effects being comparable to the herbicide metolachlor. While spraying treatment reduced the aerial and root biomass and increased the dry weight/fresh weight ratio of lettuce adult plants, watering application reduced protein contents and chlorophyll concentrations with respect to control, reflecting different modes of action depending on the site of phytotoxin entry. Via HPLC analyses, a total of 8 phenolic compounds (chlorogenic, two ρ-coumaric derivatives, ellagic, hyperoside, rutin, quercitrin, and kaempferol 3-O-glucoside and other 5 low weight organic acids (citric, malic, shikimic, succinic and fumaric acids were obtained from aqueous extract, the latter being identified for the first time in E. globulus. Despite some phytotoxic effects were found on lettuce adult plants, the use of eucalyptus

  5. Unravelling the bioherbicide potential of Eucalyptus globulus Labill: Biochemistry and effects of its aqueous extract

    Science.gov (United States)

    Reigosa, Manuel J.; Valentão, Patrícia; Andrade, Paula B.

    2018-01-01

    In the worldwide search for new strategies in sustainable weed management, the use of plant species able to produce and release phytotoxic compounds into the environment could be an effective alternative to synthetic herbicides. Eucalyptus globulus Labill. is known to be a source of biologically active compounds responsible for its phytotoxic and allelopathic properties. Our previous results demonstrated the bioherbicide potential of eucalyptus leaves incorporated into the soil as a green manure, probably through the release of phytotoxins into the soil solution. Thus, the aims of this study were to understand the phytotoxicity of the eucalyptus leaves aqueous extract applied in pre- and post-emergence, and to identify and quantify its potentially phytotoxic water-soluble compounds. The effects were tested on the germination and early growth of the model target species Lactuca sativa and Agrostis stolonifera, and on physiological parameters of L. sativa adult plants after watering or spraying application. Dose-response curves and ED50 and ED80 values for eucalyptus aqueous extracts revealed pre-emergence inhibitory effects on both target species, effects being comparable to the herbicide metolachlor. While spraying treatment reduced the aerial and root biomass and increased the dry weight/fresh weight ratio of lettuce adult plants, watering application reduced protein contents and chlorophyll concentrations with respect to control, reflecting different modes of action depending on the site of phytotoxin entry. Via HPLC analyses, a total of 8 phenolic compounds (chlorogenic, two ρ-coumaric derivatives, ellagic, hyperoside, rutin, quercitrin, and kaempferol 3-O-glucoside) and other 5 low weight organic acids (citric, malic, shikimic, succinic and fumaric acids) were obtained from aqueous extract, the latter being identified for the first time in E. globulus. Despite some phytotoxic effects were found on lettuce adult plants, the use of eucalyptus aqueous extract

  6. Water relations link carbon and oxygen isotope discrimination to phloem sap sugar concentration in eucalyptus globulus

    International Nuclear Information System (INIS)

    Cernusak, L.A.; Farquhar, G.D.; Arthur, D.J; Pate, J.S.

    2002-01-01

    Full text: The carbon isotope ratio of phloem sap sugars has been previously observed to correlate strongly with the phloem sap sugar concentration in Eucalyptus globulus. We hypothesized that the correspondence between these two parameters results from co-linearity in their responses to variation in plant water potential. Carbon isotope discrimination is expected to decrease with decreasing plant water potential due to the influence of stomatal conductance on the ratio of intercellular to ambient CO 2 , concentrations (c 1 /c a ). Conversely, we expected the phloem sap sugar concentration to increase with decreasing plant water potential, thereby maintaining positive turgor pressure within the sieve tubes. The study comprised 40 individual Eucalyptus globulus trees growing in three plantations situated on opposing ends of a rainfall gradient in southwestern Australia. A strong correlation was observed between the carbon isotope ratio in phloem sap sugars and phloem sap sugar concentration. Carbon isotope discrimination correlated positively with shoot water potential, whereas phloem sap sugar concentration correlated negatively with shoot water potential. The relationship between carbon isotope discrimination measured in phloem sap sugars collected from the stem and c 1 /c a measured instantaneously on subtending leaves was close to that theoretically predicted. Accordingly, a strong, negative relationship was observed between instantaneous c 1 /c a and the phloem sap sugar concentration. Oxygen isotope discrimination in phloem sap sugars also correlated strongly with phloem sap sugar concentration. A theoretical model suggested that the observed variation in stomatal conductance was sufficient to account for the variation observed in oxygen isotope discrimination across the study. Results strongly support the contention that water relations form a mechanistic link between phloem sap sugar concentration and both instantaneous and integrated measures of the

  7. Antifungal and antimycotoxigenic activity of essential oils from Eucalyptus globulus, Thymus capitatus and Schinus molle

    Directory of Open Access Journals (Sweden)

    Ana Karenth LÓPEZ-MENESES

    2015-12-01

    Full Text Available Abstract Essential oils (EO of eucalyptus (Eucalyptus globulus L., thymus (Thymus capitatus L. pirul (Schinus molle L. were evaluated for their efficacy to control Aspergillus parasiticus and Fusarium moniliforme growth and their ability to produce mycotoxins. Data from kinetics radial growth was used to obtain the half maximal inhibitory concentration (IC50. The IC50 was used to evaluate spore germination kinetic and mycotoxin production. Also, spore viability was evaluated by the MTT assay. All EO had an effect on the radial growth of both species. After 96 h of incubation, thymus EO at concentrations of 1000 and 2500 µL L–1 totally inhibited the growth of F. moniliforme and A. parasiticus, respectively. Eucalyptus and thymus EO significantly reduced spore germination of A. parasiticus. Inhibition of spore germination of F. moniliforme was 84.6, 34.0, and 30.6% when exposed to eucalyptus, pirul, and thymus EO, respectively. Thymus and eucalyptus EO reduced aflatoxin (4% and fumonisin (31% production, respectively. Spore viability was affected when oils concentration increased, being the thymus EO the one that reduced proliferation of both fungi. Our findings suggest that EO affect F. moniliforme and A. parasiticus development and mycotoxin production.

  8. Torrefaction of wood and bark from Eucalyptus globulus and Eucalyptus nitens: Focus on volatile evolution vs feasible temperatures

    International Nuclear Information System (INIS)

    Arteaga-Pérez, Luis E.; Segura, Cristina; Bustamante-García, Verónica; Gómez Cápiro, Oscar; Jiménez, Romel

    2015-01-01

    Torrefaction is a thermal pretreatment leading to the improvement of most of the fuel properties of biomass, namely energy density, HHV (higher heating value), grindability and hydrophobicity. The aim of this study is to identify the most feasible temperature to carry out torrefaction of Eucalyptus globulus and nitens, based on chemical evidences associated to the release of volatiles during thermal treatment of biomass. With that end: (i) Devolatilization kinetics, (ii) Effects of temperature and residence time and (iii) volatiles composition during torrefaction of both wood and bark were analyzed. In all cases DTG (derivative thermogravimetric curves) exhibited the typical shape of lignocellulosic materials, with three decomposition phases and two reaction zones. Values of activation energies for hemicellulose decomposition, were in agreement with those reported in the literature (121–170 kJ/mol). Carboxylic acids, water and phenolic compounds showed two peaks, which were associated to torrefaction (below 310 °C) and pyrolysis (310–410 °C) respectively. The most feasible temperatures for torrefaction were estimated as a function of these peaks, and it ranged between 295 °C and 310°C for all samples. Main volatile species at the torrefaction peaks were distributed as Water > Acetic Acid > CO_2 > Others, while Levoglucosan formation was marginal, due to the catalytic effect of inorganics. - Highlights: • Identification of torrefaction peaks for E. globulus and E. nitens using dynamic TGA-MS. • Devolatilization kinetics of E. globulus and E. nitens (wood and bark). • Effect of operation parameters on torrefaction of E. globulus and E. nitens. • Most feasible temperatures for torrefaction based on chemical and thermal evidences. • Definition of atomic indicator to estimate degradation degree based on TGA-MS.

  9. Eucalyptus globulus extract protects upon acetaminophen-induced kidney damages in male rat

    Science.gov (United States)

    Dhibi, Sabah; Mbarki, Sakhria; Elfeki, Abdelfettah; Hfaiedh, Najla

    2014-01-01

    Plants have historically been used in treating many diseases. Eucalyptus globules, a rich source of bioactive compounds, and have been shown to possess antioxidative properties. The purpose of this study, carried out on male Wistar rats, was to evaluate the beneficial effects of Eucalyptus globulus extract upon acetaminophen-induced damages in kidney. Our study is realized in the Department of Biology, Faculty of Sciences of Sfax (Tunisia). 32 Wistar male rats; were divided into 4 batches: a control group (n=8), a group of rats treated with acetaminophen (goomg/kg) by intraperitoneal injection during 4 days (n=8), a group receiving Eucalyptus globulus extract (130 mg of dry leaves/kg/day) in drinking water during 42 days after 2 hours of acetaminophen administration (during 4 days) (n=8) and group received only Eucalyptus (n=8) during 42 days. After 6 weeks, animals from each group were rapidly sacrificed by decapitation. Blood serum was obtained by centrifugation. Under our experimental conditions, acetaminophen poisoning resulted in an oxidative stress evidenced by statistically significant losses in the activities of catalase (CAT), superoxide-dismutase (SOD), glutathione-peroxidase (GPX) activities and an increase in lipids peroxidation level in renal tissue of acetaminophen-treated group compared with the control group. Acetaminophen also caused kidney damage as evident by statistically significant (p<0.05) increase in levels of creatinine and urea and decreased levels of uric acid and proteins in blood. Histological analysis demonstrated alteration of proximal tubules, atrophy of the glomerule and dilatation of urinary space. Previous administration of plant extract is found to alleviate this acetaminophen-induced damage. PMID:24856382

  10. Effects of essential oils from Eucalyptus globulus leaves on soil organisms involved in leaf degradation.

    Directory of Open Access Journals (Sweden)

    Carla Martins

    Full Text Available The replacement of native Portuguese forests by Eucalyptus globulus is often associated with deleterious effects on terrestrial and aquatic communities. Several studies have suggested that such a phenomenon is linked with the leaf essential oils released into the environment during the Eucalyptus leaf degradation process. However, to date, the way these compounds affect leaf degradation in terrestrial systems i.e. by direct toxic effects to soil invertebrates or indirectly by affecting food of soil fauna, is still unknown. In order to explore this question, the effect of essential oils extracted from E. globulus leaves on terrestrial systems was investigated. Fungal growth tests with species known as leaf colonizers (Mucor hiemalis, Alternaria alternata, Penicillium sp., Penicillium glabrum and Fusarium roseum were performed to evaluate the antifungal effect of essential oils. In addition, a reproduction test with the collembolans Folsomia candida was done using a gradient of eucalyptus essential oils in artificial soil. The influence of essential oils on feeding behaviour of F. candida and the isopods Porcellio dilatatus was also investigated through food avoidance and consumption tests. Eucalyptus essential oils were lethal at concentrations between 2.5-20 µL/mL and inhibited growth of all fungal species between 1.25-5 µL/mL. The collembolan reproduction EC50 value was 35.0 (28.6-41.2 mg/kg and both collembola and isopods preferred leaves without oils. Results suggested that the effect of essential oils in leaf processing is related to direct toxic effects on fungi and soil fauna and to indirect effects on the quality and availability of food to soil invertebrates.

  11. The Role of Eucalyptus Globulus Forest and Products in Carbon Sequestration

    International Nuclear Information System (INIS)

    Arroja, L.; Dias, A.C.; Capela, I.

    2006-01-01

    This study is a contribution to the ongoing debate about the selection of the approach for carbon accounting in wood products to be used, in the future, in the national greenhouse gas inventories under the UNFCCC (United Nations Framework Convention on Climate Change). Two accounting approaches are used in this analysis: the stock-change approach and the atmospheric-flow approach. They are applied to the Portuguese Eucalyptus globulus forest sector. To achieve this objective, the fluxes of wood removed from the forest are tracked through its life cycle, which includes products manufacture (mainly pulp and paper), use and final disposal (landfilling, incineration and composting). This study develops a framework to the estimation of carbon sequestration in the forest of E. globulus, a fast growing species, more specifically, in the calculation of the conversion factors such as bark and foliage percentages and densities, used to convert wood volumes into total biomass. A mass balance approach based on real data from mills is also proposed, in order to assess carbon emissions from wood processing. The results show that E. globulus forest sector was a carbon sink, but the magnitude of the carbon sequestration differs substantially depending on the accounting approach used. The contribution of the forest ecosystem was smaller than the aggregated contribution of wood products in use and in landfills (including industrial waste), which reinforces the role that wood products play in national carbon budgets

  12. A model for predicting the growth of Eucalyptus globulus seedling stands in Bolivia

    Energy Technology Data Exchange (ETDEWEB)

    Guzman, G.; Morales, M.; Pukkala, T.; Miguel, S. de

    2012-11-01

    Eucalyptus globulus is one of the most planted species in the Inter-Andean Valleys of Bolivia, where growing conditions are different from most places where eucalyptus have been studied. This prevents a straightforward utilization of models fitted elsewhere. In this study a distance-independent individual-tree growth model for E. globulus plantations in Bolivia was developed based on data from 67 permanent sample plots. The model consists of sub-models for dominant height, tree diameter increment, height-diameter relationship and survival. According to model-based simulations, the mean annual increment with the optimal rotation length is about 13 m3 ha{sup -}1 yr{sup -}1 on medium-quality sites and 18 m{sup 3} ha{sup -}1 yr -1 on the best sites. A suitable rotation length for maximizing wood production is approximately 30 years on medium sites and 20 years on the most productive sites. The developed models provide valuable information for further studies on optimizing the management and evaluating alternative management regimes for the species. (Author) 22 refs.

  13. Physiological and biochemical responses to severe drought stress of nine Eucalyptus globulus clones: a multivariate approach.

    Science.gov (United States)

    Granda, Víctor; Delatorre, Carolina; Cuesta, Candela; Centeno, María L; Fernández, Belén; Rodríguez, Ana; Feito, Isabel

    2014-07-01

    Seasonal drought, typical of temperate and Mediterranean environments, creates problems in establishing plantations and affects development and yield, and it has been widely studied in numerous species. Forestry fast-growing species such as Eucalyptus spp. are an important resource in such environments, selected clones being generally used for production purposes in plantations in these areas. However, use of mono-specific plantations increases risk of plant loss due to abiotic stresses, making it essential to understand differences in an individual clone's physiological responses to drought stress. In order to study clonal differences in drought responses, nine Eucalyptus globulus (Labill.) clones (C14, C46, C97, C120, C222, C371, C405, C491 and C601) were gradually subjected to severe drought stress (<14% of field capacity). A total of 31 parameters, physiological (e.g., photosynthesis, gas exchange), biochemical (e.g., chlorophyll content) and hormonal (abscisic acid [ABA] content), were analysed by classic and multivariate techniques. Relationships between parameters were established, allowing related measurements to be grouped into functional units (pigment, growth, water and ABA). Differences in these units showed that there were two distinct groups of E. globulus clones on the basis of their different strategies when faced with drought stress. The C14 group (C14, C120, C405, C491 and C601) clones behave as water savers, maintaining high water content and showing high stomatal adjustment, and reducing their aerial growth to a great extent. The C46 group (C46, C97, C222 and C371) clones behave as water spenders, reducing their water content drastically and presenting osmotic adjustment. The latter maintains the highest growth rate under the conditions tested. The method presented here can be used to identify appropriate E. globulus clones for drought environments, facilitating the selection of material for production and repopulation environments. © The

  14. Are post-dispersed seeds of Eucalyptus globulus predated in the introduced range? Evidence from an experiment in Portugal

    Directory of Open Access Journals (Sweden)

    E. Deus

    2018-04-01

    Full Text Available Plantations of Eucalyptus globulus Labill. have been expanding rapidly worldwide. The species is considered invasive in several regions. While in the native range, post-dispersal seed predation is known to severely limit eucalypt recruitment, there is no experimental evidence of seed predation in the introduced range. We hypothesised that E. globulus seeds largely escape predation in Portugal, which may explain its prolific recruitment in some locations. We tested this hypothesis in central Portugal by exposing E. globulus seeds to the local fauna. For comparison purposes, we also used seeds from locally common species: Acacia dealbata Link (alien, larger, elaiosome-bearing seeds and Cistus salviifolius L. (native, similarly sized seeds. We installed 30 feeding stations across three study sites, each one dominated by one study species. Each feeding station featured four feeders with different animal-access treatments: invertebrates; vertebrates; full access; no access (control. We placed five seeds of each plant species every day in each feeder and registered the number of seeds missing, eaten and elaiosome detached over 9 summer days. Eucalyptus globulus seeds were highly attractive to fauna in the three sites. Nearly half of E. globulus seeds were predated or removed, thus contradicting our hypothesis. Surprisingly, E. globulus and A. dealbata seeds were used by animals in similar proportions and C. salviifolius seeds were the least preferred. Vertebrates were the predominant seed predators and preferred the alien seeds. Invertebrates used all seed species in similar proportions. We found spatial variation regarding the predominant type of seed predators and the levels of seed predation according to the following patterns: predominance of vertebrates; predominance of invertebrates; negligible seed predator activity. Locations with negligible seed predation were abundant and scattered across the study area. Such spatial variation may

  15. Adaptación de la madera de Eucalyptus globulus a la normativa europea de durabilidad natural

    OpenAIRE

    Lorenzo, D.; Troya, M. T. de,; Baso, Carlos; Touza, M.; Prieto, M.J.

    2007-01-01

    La madera de Eucalyptus globulus en algunos países como Australia es recomendada para situaciones de riesgo de ataque de organismos xilófagos por su elevada durabilidad. En Galicia esta madera ha sido utilizada desde hace muchas décadas en estructuras y carpinterías, que aún hoy perduran. Sin embargo, la norma europea EN-350-2 encuadra la especie E. globulus en la peor categoría de durabilidad natural, situación que no se corresponde con la realidad. El objetivo de este proyecto es constatar ...

  16. Events Associated with Early Age-Related Decline in Adventitious Rooting Competence of Eucalyptus globulus Labill.

    Science.gov (United States)

    Aumond, Márcio L; de Araujo, Artur T; de Oliveira Junkes, Camila F; de Almeida, Márcia R; Matsuura, Hélio N; de Costa, Fernanda; Fett-Neto, Arthur G

    2017-01-01

    The development of adventitious roots is affected by several factors, including the age of the cutting donor plant, which negatively affects rooting capacity. Eucalyptus globulus quickly loses rooting capacity of cuttings as the donor plant ages, although the molecular and biochemical mechanisms behind this process are still unclear. To better understand the bases of rooting competence loss in E. globulus , the time required for a significant decline in rhizogenic ability without exogenous auxin was determined in microcuttings derived from donor plants of different ages after sowing. Tip cuttings of donor plants were severed before and after loss of rooting competence of microcuttings to test the hypothesis that auxin and carbohydrate homeostasis regulate rooting competence decline. There were no significant changes in concentration of carbohydrates, flavonoids, or proteins before and after the loss of rooting capacity. Peroxidase (EC 1.11.1.7) total activity increased with loss of rooting competence. Auxin concentration showed the opposite pattern. In good agreement, TAA1 , a key gene in auxin biosynthesis, had lower expression after loss of rooting capacity. The same applied to the auxin receptor gene TIR1 , suggesting reduced auxin sensitivity. On the other hand, genes associated with auxin response repression ( TPL , IAA12 ) or with the action of cytokinins, the rhizogenesis inhibitor-related ARR1 , showed higher expression in plants with lower rooting competence. Taken together, data suggest that age negatively affects E. globulus rooting by a combination of factors. Decreased endogenous auxin concentration, possibly caused by less biosynthesis, lower auxin sensitivity, higher expression of genes inhibiting auxin action, as well as of genes related to the action of cytokinins, appear to play roles in this process.

  17. Events Associated with Early Age-Related Decline in Adventitious Rooting Competence of Eucalyptus globulus Labill

    Science.gov (United States)

    Aumond, Márcio L.; de Araujo, Artur T.; de Oliveira Junkes, Camila F.; de Almeida, Márcia R.; Matsuura, Hélio N.; de Costa, Fernanda; Fett-Neto, Arthur G.

    2017-01-01

    The development of adventitious roots is affected by several factors, including the age of the cutting donor plant, which negatively affects rooting capacity. Eucalyptus globulus quickly loses rooting capacity of cuttings as the donor plant ages, although the molecular and biochemical mechanisms behind this process are still unclear. To better understand the bases of rooting competence loss in E. globulus, the time required for a significant decline in rhizogenic ability without exogenous auxin was determined in microcuttings derived from donor plants of different ages after sowing. Tip cuttings of donor plants were severed before and after loss of rooting competence of microcuttings to test the hypothesis that auxin and carbohydrate homeostasis regulate rooting competence decline. There were no significant changes in concentration of carbohydrates, flavonoids, or proteins before and after the loss of rooting capacity. Peroxidase (EC 1.11.1.7) total activity increased with loss of rooting competence. Auxin concentration showed the opposite pattern. In good agreement, TAA1, a key gene in auxin biosynthesis, had lower expression after loss of rooting capacity. The same applied to the auxin receptor gene TIR1, suggesting reduced auxin sensitivity. On the other hand, genes associated with auxin response repression (TPL, IAA12) or with the action of cytokinins, the rhizogenesis inhibitor-related ARR1, showed higher expression in plants with lower rooting competence. Taken together, data suggest that age negatively affects E. globulus rooting by a combination of factors. Decreased endogenous auxin concentration, possibly caused by less biosynthesis, lower auxin sensitivity, higher expression of genes inhibiting auxin action, as well as of genes related to the action of cytokinins, appear to play roles in this process. PMID:29067033

  18. Ecophysiological responses of a young blue gum (Eucalyptus globulus) plantation to weed control.

    Science.gov (United States)

    Eyles, Alieta; Worledge, Dale; Sands, Peter; Ottenschlaeger, Maria L; Paterson, Steve C; Mendham, Daniel; O'Grady, Anthony P

    2012-08-01

    Early weed control may improve the growth of forest plantations by influencing soil water and nutrient availability. To understand eucalypt growth responses to weed control, we examined the temporal responses of leaf gas-exchange, leaf nitrogen concentration (N) and water status of 7-month-old Eucalyptus globulus L. trees in a paired-plot field trial. In addition, we monitored the growth, leaf N and water status of the competing vegetation in the weed treatment. By the end of the 11-month experiment, complete weed control (WF treatment) of largely woody competitors increased the basal diameter of E. globulus by 14%. As indicated by pre-dawn water potentials of > - 0.05 MPa, interspecies competition for water resources was minimal at this site. In contrast, competition for N appeared to be the major factor limiting growth. Estimations of total plot leaf N (g m(-2) ground) showed that competing vegetation accounted for up to 70% of the total leaf N at the start of the trial. This value fell to 15% by the end of the trial. Despite increased leaf N(area) in WF trees 5 months after imposition of weed control, the photosynthetic capacity (A(1500)) of E. globulus was unaffected by treatment suggesting that the growth gains from weed control were largely unrelated to changes in leaf-level photosynthesis. Increased nutrient availability brought about by weed control enabled trees to increase investment into leaf-area production. Estimates of whole-tree carbon budget based on direct measurements of dark respiration and A(1500) allowed us to clearly demonstrate the importance of leaf area driving greater productivity following early weed control in a nutrient-limited site.

  19. Methodology for logistic planning of forest roads for harvesting Eucalyptus globulus Labill. using optimization tools

    International Nuclear Information System (INIS)

    Leal Robert, Orlando; Lopez, Cesar Amilcar

    2011-01-01

    Using an un capacitated and non directed network model, we propose a methodology for the preliminary design of second order forests roads to extract products from Eucalyptus globulus plantations using an aerial cable system to minimize road network construction costs. The model takes into account topographical conditions and road construction costs related to the terrain slope. In order to solve the problem the Dijstrak algorithm was used, in which the minimum cost of the shortest paths that connect all the stockyards are defined. In addition, the Kruskal algorithm was used to obtain the minimum spanning tree. Finally, the network was connected to an open road for the timber to be taken to consumption centers. In order to develop the algorithms mentioned above, an application on Mat lab was designed, which gave an easier handling of the cartography data obtained from these algorithms.

  20. Macrocarpal C isolated from Eucalyptus globulus inhibits dipeptidyl peptidase 4 in an aggregated form.

    Science.gov (United States)

    Kato, Eisuke; Kawakami, Kazuhiro; Kawabata, Jun

    2018-12-01

    Dipeptidyl peptidase 4 (DPP-4) inhibitors are used for the treatment of type-2 diabetes mellitus. Various synthetic inhibitors have been developed to date, and plants containing natural DPP-4 inhibitors have also been identified. Here, 13 plant samples were tested for their DPP-4 inhibitory activity. Macrocarpals A-C were isolated from Eucalyptus globulus through activity-guided fractionation and shown to be DPP-4 inhibitors. Of these, macrocarpal C showed the highest inhibitory activity, demonstrating an inhibition curve characterised by a pronounced increase in activity within a narrow concentration range. Evaluation of macrocarpal C solution by turbidity, nuclear magnetic resonance spectroscopy and mass spectrometry indicated its aggregation, which may explain the characteristics of the inhibition curve. These findings will be valuable for further study of potential small molecule DPP-4 inhibitors.

  1. RECOVERY OF LOGGING RESIDUES THROUGH BALER ON FORWARDER: EXPERIENCE WITH Eucalyptus globulus IN CHILE

    Directory of Open Access Journals (Sweden)

    Eduardo Acuña

    2018-05-01

    Full Text Available ABSTRACT This study presents results of tests on the recovery of Eucalyptus globulus logging residues. Leftovers were packed with an ENFO-2002 baler on a forwarder, taking advantage of a cut-to-length harvesting system for two conditions of residual biomass, i.e., with and without bark. A continuous field study was carried out, controlling 16.4 scheduled hours in seven days. Delays in the operation stemmed mainly by the visits of the client company and supervision by phone. The baler had an average utilization capacity of 81% for both evaluated conditions and produced an average of 36.2 bales with and 22.2 bales without bark per productive area. An average dry biomass productivity of 5.85 and 5.46 Mg h-1 was reached for similar conditions of residual biomass. Significant differences emerged when the type of harvesting was compared to mean dry weight, between types of harvesting, and mean baling time. Thus, the presence of bark in the logging residues of E. globulus increases the productivity of the baler.

  2. Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production

    Science.gov (United States)

    Domingues, Rui M. A.; Oliveira, Eduardo L. G.; Freire, Carmen S. R.; Couto, Ricardo M.; Simões, Pedro C.; Neto, Carlos P.; Silvestre, Armando J. D.; Silva, Carlos M.

    2012-01-01

    Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids). In this work, the supercritical fluid extraction (SFE) of Eucalyptus globulus deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100–200 bar), co-solvent (ethanol) content (0, 5 and 8% wt), and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by several tens of bar. At 160 bar and 40 °C, the introduction of 8% (wt) of ethanol greatly improves the yield of triterpenoids more than threefold. PMID:22837719

  3. Linking photosynthesis and leaf N allocation under future elevated CO2 and climate warming in Eucalyptus globulus

    Science.gov (United States)

    Sharwood, Robert E.; Crous, Kristine Y.; Whitney, Spencer M.; Ellsworth, David S.

    2017-01-01

    Abstract Leaf-level photosynthetic processes and their environmental dependencies are critical for estimating CO2 uptake from the atmosphere. These estimates use biochemical-based models of photosynthesis that require accurate Rubisco kinetics. We investigated the effects of canopy position, elevated atmospheric CO2 [eC; ambient CO2 (aC)+240 ppm] and elevated air temperature (eT; ambient temperature (aT)+3 °C) on Rubisco content and activity together with the relationship between leaf N and Vcmax (maximal Rubisco carboxylation rate) of 7 m tall, soil-grown Eucalyptus globulus trees. The kinetics of E. globulus and tobacco Rubisco at 25 °C were similar. In vitro estimates of Vcmax derived from measures of E. globulus Rubisco content and kinetics were consistent, although slightly lower, than the in vivo rates extrapolated from gas exchange. In E. globulus, the fraction of N invested in Rubisco was substantially lower than for crop species and varied with treatments. Photosynthetic acclimation of E. globulus leaves to eC was underpinned by reduced leaf N and Rubisco contents; the opposite occurred in response to eT coinciding with growth resumption in spring. Our findings highlight the adaptive capacity of this key forest species to allocate leaf N flexibly to Rubisco and other photosynthetic proteins across differing canopy positions in response to future, warmer and elevated [CO2] climates. PMID:28064178

  4. EFFECT OF LIGNIN CONTENT OF Eucalyptus globulus WOOD IN KRAFT PULPING PERFORMANCE

    Directory of Open Access Journals (Sweden)

    Gabriel Valim Cardoso

    2011-03-01

    Full Text Available In this research, it was analyzed the lignin content effect of Eucalyptus globulus wood in kraft pulping optimization. Seventy-two laboratory cooking were made with wood chips obtained from six Eucalyptus globulus trees selected from a group of 50 trees. The wood chips from three trees with the lowest lignin content, with average 20,53%, were mixed proportionally based on the tree weights, obtaining the sample of low lignin content wood. The same was made to obtain the sample for wood chips with the highest lignin content, with average 23,02%. The two lignin levels were statistically different. The two wood samples had basic densities statistically not different. Using three maximum temperatures levels (160, 165 and 170ºC, and three active alkali charged (17, 18,5 and 20%, the wood chips were converted to kraft pulps. The pulps were then characterized to analyze the influence of the distinct treatments employed in the cooking on their properties. The effect of the cooking conditions was expressed by mathematical models in order to determine the optimum points for each of the evaluated properties. The optimization process indicated maximum temperature of 168ºC, and active alkali of 19%, for maximum kraft pulping yield to achieve kappa number 18; this result was for woods with low lignin content. For woods containing the high lignin content, the optimization showed maximum cooking temperature of 169ºC and active alkali of 19% for kappa number of 18. The average reduction of 2,49% in wood lignin content resulted a correspondent gain of 2,2% in the kraft yield (o.d. basis and a reduction on the active alkali charge of 1,2% (o.d. basis to achieve kappa numbers from 16 to 19, preserving pulp properties. If the option is to work with kappa number 19 instead of 16, the gain in kraft yield is approximately 2%. Therefore, when working with low lignin content wood and kappa number 19 instead of 16, a substantial gain of approximately 4,2% is obtained

  5. Modeling and optimization of extraction process of eucalyptus essential oil (Eucalyptus globulus

    Directory of Open Access Journals (Sweden)

    Jeancarlos Moreno

    2010-06-01

    Full Text Available In this paper we have studied the effect of the moisture from the eucalyptus leaf and the time of extraction on the yield of essential oil and the production cost. We took into account parameters such as steam temperature, pressure extraction, porous bed, steam flow and temperature of condensation. A Central Composite Rotational Design was used to evaluate optimum extraction areas and the cost that would follow, searching always to increase yields and reduce costs. According to this design, the extraction with the lowest production costs (0.57 Nuevos Soles by mL of essential oil is reached when the leaves have moisture between 25 and 30% and are extracted with times between 98 and 126 min

  6. Water stress and recovery in the performance of two Eucalyptus globulus clones: physiological and biochemical profiles.

    Science.gov (United States)

    Correia, Barbara; Pintó-Marijuan, Marta; Neves, Lucinda; Brossa, Ricard; Dias, Maria Celeste; Costa, Armando; Castro, Bruno B; Araújo, Clara; Santos, Conceição; Chaves, Maria Manuela; Pinto, Glória

    2014-04-01

    Eucalyptus plantations are among the most productive forest stands in Portugal and Spain, being mostly used for pulp production and, more recently, as an energy crop. However, the region's Mediterranean climate, with characteristic severe summer drought, negatively affects eucalypt growth and increases mortality. Although the physiological response to water shortage is well characterized for this species, evidence about the plants' recovery ability remains scarce. In order to assess the physiological and biochemical response of Eucalyptus globulus during the recovery phase, two genotypes (AL-18 and AL-10) were submitted to a 3-week water stress period at two different intensities (18 and 25% of field capacity), followed by 1 week of rewatering. Recovery was assessed 1 day and 1 week after rehydration. Drought reduced height, biomass, water potential, NPQ and gas exchange in both genotypes. Contrarily, the levels of pigments, chlorophyll fluorescence parameters (F(v) /F(m) and (φPSII)), MDA and ABA increased. During recovery, the physiological and biochemical profile of stressed plants showed a similar trend: they experienced reversion of altered traits (MDA, ABA, E, g(s), pigments), while other parameters did not recover ((φPSII), NPQ). Furthermore, an overcompensation of CO(2) assimilation was achieved 1 week after rehydration, which was accompanied by greater growth and re-establishment of oxidative balance. Both genotypes were tolerant to the tested conditions, although clonal differences were found. AL-10 was more productive and showed a more rapid and dynamic response to rehydration (namely in carotenoid content, (φPSII) and NPQ) compared to clone AL-18. © 2013 Scandinavian Plant Physiology Society.

  7. [Ovicidal and larvicidal activity in vitro of Eucalyptus globulus essential oils on Haemonchus contortus].

    Science.gov (United States)

    Macedo, Iara T F; Bevilaqua, Claudia M L; de Oliveira, Lorena M B; Camurça-Vasconcelos, Ana L F; Vieira, Luiz da S; Oliveira, Fabrício R; Queiroz-Junior, Eudson M; Portela, Bruno G; Barros, Renata S; Chagas, Ana C S

    2009-01-01

    The objective of this work was to evaluate ovicidal and larvicidal effects of Eucalyptus globulus essential oil (EGEO) on Haemonchus contortus. The chemical composition determination of EGEO was through gas chromatography and mass spectrometry. Egg hatch test (EHT) was performed in concentrations 21.75; 17.4; 8.7; 5.43 e 2.71 mg x mL(-1). In larval development test (LDT) were used the concentrations 43.5; 21.75; 10.87; 5.43 e 2.71 mg x mL(-1). Each trial was conducted by negative control with Tween 80 (3%) and positive control, 0.02 mg x mL(-1) of thiabendazole in EHT and 0.008 mg x mL(-1) of ivermectin in LDT. The maximum effectiveness of EGEO on eggs was 99.3% in concentration of 21.75 mg x mL(-1) and on larvae was 98.7% in concentration 43.5 mg x mL(-1). The concentration of EGEO that inhibits 50% of the eggs and larvae was 8.3 and 6.92 mg x mL(-1), respectively. The oil chemical analysis identified as main component the monoterpen 1,8-cineol. EGEO presented ovicidal and larvicidal activities in vitro, revealing a good potential for use in the control of sheep and goat gastrointestinal nematodes.

  8. Enhancement of enzymatic saccharification of Eucalyptus globulus: steam explosion versus steam treatment.

    Science.gov (United States)

    Martin-Sampedro, Raquel; Revilla, Esteban; Villar, Juan C; Eugenio, Maria E

    2014-09-01

    Steam explosion and steam pre-treatment have proved capable of enhancing enzymatic saccharification of lignocellulosic materials. However, until now, these methods had not been compared under the same operational conditions and using the same raw material. Both pre-treatments lead to increased yields in the saccharification of Eucalyptus globulus; but results have been better with steam pre-treatments, despite the more accessible surface of exploded samples. The reason for this finding could be enzymatic inhibition: steam explosion causes a more extensive extraction of hemicelluloses and releases a greater amount of degradation products which can inhibit enzymatic action. Enzymatic inhibition is also dependent on the amount and chemical structure of lignin, which was also a contributing factor to the lower enzymatic yields obtained with the most severe pre-treatment. Thus, the highest yields (46.7% glucose and 73.4% xylose yields) were obtained after two cycle of steam treatment, of 5 and 3 min, at 183°C. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Steam torrefaction of Eucalyptus globulus for producing black pellets: A pilot-scale experience.

    Science.gov (United States)

    Arteaga-Pérez, Luis E; Grandón, Héctor; Flores, Mauricio; Segura, Cristina; Kelley, Stephen S

    2017-08-01

    Steam torrefaction of Eucalyptus globulus was performed at temperatures between 245°C and 265°C in a 100kg/h pilot plant. Torrefied biomass was then pelletized in a 300kg/h unit and the pellets were subject to durability, density and combustion tests. The structural changes measured with FTIR were studied along with the combustion behavior of the materials. Compositional analysis showed that increasing the torrefaction temperature reduced both hemicellulose fraction and overall mass yield (MY). Furthermore, there was a linear relationship between the energy yield (EY) and mass yield (EY=[1.04-0.9(1-MY)]) for these samples. The ignition and comprehensive indexes confirmed that the stability of the torrefied biomass in a combustion environment was higher than for untreated biomass. Finally, pellets showed high durability (98%), and had an energy density (13-14GJ/m 3 ), which is comparable to low-rank coals. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Green Synthesis of Silver Nanoparticles by using Eucalyptus Globulus Leaf Extract

    Science.gov (United States)

    Balamurugan, Madheswaran; Saravanan, Shanmugam

    2017-12-01

    A single step eco-friendly, energy efficient and economically scalable green method was employed to synthesize silver nanoparticles. In this work, the synthesis of silver nanoparticles using Eucalyptus globulus leaf extract as reducing and capping agent along with water as solvent at normal room temperature is described. Silver nanoparticles were prepared from aqueous silver nitrate solution by adding the leaf extract. The prepared nanoparticles were characterized by using UV-visible Spectrophotometer, X-ray diffractometer, High Resolution Transmission Electron Microscope (HR-TEM) and Fourier Transform Infrared Spectroscope (FTIS). X-ray diffraction studies brought to light the crystalline nature and the face centered cubic structure of the silver nanoparticles. Using HR-TEM. the nano sizes and morphology of the particles were studied. The mean sizes of the prepared silver nanoparticles ranged from 30 to 36 nm. The density of the particles was tuned by varying the molar ratio of silver nitrate. FTIS studies showed the functional group of organic molecules which were located on the surface of the silver nanoparticles. Originating from the leaf extracts, these organic molecules reduced and capped the particles.

  11. BAYESIAN PREDICTION OF GENETIC PARAMETERS IN Eucalyptus globulus CLONES UNDER WATER SUPPLY CONDITIONS

    Directory of Open Access Journals (Sweden)

    Freddy Mora

    2013-06-01

    Full Text Available http://dx.doi.org/10.5902/198050989297A Bayesian analysis of genetic parameters for growth traits at twelve months after planting was carried out in twenty nine Eucalyptus globulus clones in southern Chile. Two different environmental conditions were considered: 1 Non-irrigation and; 2 Plants were irrigated with a localized irrigation system. The Bayesian approach was performed using Gibbs sampling algorithm in a clone-environment interaction model. Inheritability values ​​were high in the water supply condition (posterior mode: H2=0.41, 0.36 and 0.39 for height, diameter and sectional area, respectively, while in the environment without irrigation, the inheritabilities were significantly lower, which was confirmed by the Bayesian credible intervals (95% probability. The posterior mode of the genetic correlation between sites was positive and high for all traits (r=0.7, 0.65 and 0.8, for height, diameter and sectional area, respectively and according to the credible interval, it was statistically different from zero, indicating a non-significant interaction.

  12. CARACTERIZACION DE GENES DESHIDRINA EN EUCALYPTUS GLOBULUS CUYA EXPRESION ES INDUCIDA POR LA ACLIMATACION A BAJA TEMPERATURA

    OpenAIRE

    FERNANDEZ REYES, MARTA ALEJANDRA

    2011-01-01

    Eucalyptus globulus es una especie importante para la producción de pulpa de madera en Chile, sin embargo presenta una gran sensibilidad a temperaturas de congelamiento. Durante los últimos años, muchos estudios han enfocado sus esfuerzos en dilucidar los mecanismos que regulan la respuesta de las plantas a baja temperatura, mediante el análisis de la expresión génica. En el presente trabajo se estudia la expresión de genes deshidrina durante el proceso de aclimatación a baja temperatura y...

  13. CARACTERIZACION DE GENES DESHIDRINA EN EUCALYPTUS GLOBULUS CUYA EXPRESION ES INDUCIDA POR LA ACLIMATACION A BAJA TEMPERATURA

    OpenAIRE

    FERNANDEZ REYES ; MARTA ALEJANDRA; FERNANDEZ REYES ; MARTA ALEJANDRA

    2011-01-01

    Eucalyptus globulus es una especie importante para la producción de pulpa de madera en Chile, sin embargo presenta una gran sensibilidad a temperaturas de congelamiento. Durante los últimos años, muchos estudios han enfocado sus esfuerzos en dilucidar los mecanismos que regulan la respuesta de las plantas a baja temperatura, mediante el análisis de la expresión génica. En el presente trabajo se estudia la expresión de genes deshidrina durante el proceso de aclimatación a baja temperatura y la...

  14. Efeito do óleo essencial de Eucalyptus globulus sobre espécies produtoras de aflatoxinas

    OpenAIRE

    Georgia Rocha Vilela

    2007-01-01

    Há relatos na literatura de alguns compostos naturais de plantas que são usados para preservação de alimentos e no controle do desenvolvimento de fungos e bactérias que ocorrem em plantas, grãos, cereais e derivados. O presente trabalho teve como objetivo avaliar "in vitro" o efeito do óleo essencial de Eucalyptus globulus e seu composto majoritário sobre o crescimento micelial dos fungos Aspergillus flavus e Aspergillus parasiticus e a produção de aflatoxinas. A composição química do...

  15. MODELACIÓN DEL EFECTO DE LA PODA Y RALEO SOBRE LAS DEFORMACIONES RESIDUALES LONGITUDINALES EN Eucalyptus globulus Labill.

    OpenAIRE

    Eduardo Navarrete; Carolina Valenzuela; Leif Nutto

    2016-01-01

    RESUMEN Se estudió una plantación de Eucalyptus globulus ubicada en el sector precordillerano andino de la región del Bío Bío, Chile, c on el objetivo de evaluar y modelar el efecto de la poda y raleo sobre las deformaciones residuales longitudinales (DRL) . El lugar de estudio correspondió a un rodal de 12 años de edad, el cual fue intervenido a los 2 años y medio de establecido, donde se evaluaron distintas intensidades de poda (0% y 60% de la altura total) y densidades de raleo (1.6...

  16. MODELACIÓN DEL EFECTO DE LA PODA Y RALEO SOBRE LAS DEFORMACIONES RESIDUALES LONGITUDINALES EN Eucalyptus globulus Labill.

    OpenAIRE

    Navarrete, Eduardo; Valenzuela, Carolina; Nutto, Leif

    2016-01-01

    RESUMEN Se estudió una plantación de Eucalyptus globulus ubicada en el sector precordillerano andino de la región del Bío Bío, Chile, con el objetivo de evaluar y modelar el efecto de la poda y raleo sobre las deformaciones residuales longitudinales (DRL). El lugar de estudio correspondió a un rodal de 12 años de edad, el cual fue intervenido a los 2 años y medio de establecido, donde se evaluaron distintas intensidades de poda (0% y 60% de la altura total) y densidades de raleo (1.600 árb·ha...

  17. Resource Communication. Temporal optimization of fuel treatment design in blue gum (Eucalyptus globulus) plantations

    Energy Technology Data Exchange (ETDEWEB)

    Martin, A.; Botequim, B.; Oliveira, T.M.; Ager, A.; Pirotti, F.

    2016-07-01

    Aim of the study: This study was conducted to support fire and forest management planning in eucalypt plantations based on economic, ecological and fire prevention criteria, with a focus on strategic prioritisation of fuel treatments over time. The central objective was to strategically locate fuel treatments to minimise losses from wildfire while meeting budget constraints and demands for wood supply for the pulp industry and conserving carbon. Area of study: The study area was located in Serra do Socorro (Torres Vedras, Portugal, covering ~1449 ha) of predominantly Eucalyptus globulus Labill forests managedcultivated for pulpwood by The Navigator Company. Material and methods: At each of four temporal stages (2015-2018-2021-2024) we simulated: (1) surface and canopy fuels, timber volume (m3 ha-1) and carbon storage (Mg ha-1); (2) fire behaviour characteristics, i.e. rate of spread (m min-1), and flame length (m), with FlamMap fire modelling software; (3) optimal treatment locations as determined by the Landscape Treatment Designer (LTD). Main results: The higher pressure of fire behaviour in the earlier stages of the study period triggered most of the spatial fuel treatments within eucalypt plantations in a juvenile stage. At later stages fuel treatments also included shrublands areas. The results were consistent with observations and simulation results that show high fire hazard in juvenile eucalypt stands. Research highlights: Forest management planning in commercial eucalypt plantations can potentially accomplish multiple objectives such as augmenting profits and sustaining ecological assets while reducing wildfire risk at landscape scale. However, limitations of simulation models including FlamMap and LTD are important to recognise in studies of long term wildfire management strategies. (Author)

  18. EFECTO DE LA DENSIDAD DE PLANTACIÓN EN LA RENTABILIDAD DE PLANTACIONES DE Eucalyptus globulus

    Directory of Open Access Journals (Sweden)

    Emilio Guerra-Bugueño

    2014-01-01

    Full Text Available El estudio evaluó, económicamente, la respuesta de densidades de plantación y marcos de espaciamiento de Eucalyptus globulus establecido con fines pulpables. La técnica de Montecarlo se usó para examinar el efecto de las variaciones de precios y la tasa de descuento sobre la rentabilidad. El ensayo fue realizado en el Valle Central de la IX Región de Chile. Los tratamientos fueron: 1,000 árboles·ha-1(T1; 1,428 árboles·ha-1 (T2; 1,667 árboles·ha-1 (T3 y 2,000 árboles·ha-1 (T4. El volumen de producción de cada tratamiento se midió a la edad de cosecha (10 años y posteriormente se realizó un análisis financiero, para obtener la rentabilidad (valor actual neto [VAN], valor económico del suelo [VES] y tasa interna de retorno [TIR]. Los mayores valores de VAN se obtuvieron con el T1 (US$ 330·ha-1 y T4 (US$ 322·ha-1. Los pronósticos obtenidos para el VAN de T1 mostraron una probabilidad de ocurrencia de 73 %, resultando ser la opción menos riesgosa para la inversión. Los mayores rendimientos volumétricos (T4 y T3 no propician las mayores rentabilidades, pues el incremento de la densidad de plantación aumenta los costos por unidad de planta con relación al manejo de suelos, control de malezas y fertilización.

  19. Control of Botrytis cinerea in Eucalyptus globulus Mini-Cuttings Using Clonostachys and Trichoderma Strains Control de Botrytis cinerea en miniestacas de Eucalyptus globulus Utilizando Cepas de Clonostachys y Trichoderma

    Directory of Open Access Journals (Sweden)

    Salomé Zaldúa

    2010-12-01

    Full Text Available Botrytis cinerea Pers. ex Fr. causes the disease known as gray mold in more than 200 hosts. It is one of the most important pathogens in Chilean forest nurseries and Eucalyptus globulus Labill. is one of the most susceptible species, especially in vegetative reproduction systems. Clonostachys and Trichoderma strains were selected as potential biocontrol agents of gray mold in previous research by the authors. The objective of this study was to evaluate the effectiveness of antagonistic fungi to control B. cinerea in E. globulus mini-cuttings. Five fungi strains were tested and applied weekly, two Clonostachys and three Trichoderma (5 x 10(6 conidia mL-1. In addition, comparison treatments were also used: absolute control (water and fungicide application. The experiment was carried out under operational conditions to produce E. globulus mini-cuttings. The Clonostachys UDC-A10 and UDC-A11 strains reduce mini-cutting mortality caused by B. cinerea in 54 and 71%, respectively, and with effects similar to those achieved by fungicides. Clonostachys UDC-A11 reduces the disease progression rate with the same statistical results as fungicides. A negative effect of applying fungicides on rooting of the surviving mini-cuttings was also confirmed. These results demonstrate the effectiveness of Clonostachys as a control agent against gray mold disease in E. globulus mini-cuttings.Botrytis cinerea Pers. ex Fr. ocasiona la enfermedad conocida como moho gris en más de 200 hospederos. En Chile es uno de los patógenos más importantes en viveros forestales, siendo Eucalyptus globulus Labill. una de las especies más susceptibles, especialmente en los sistemas de reproducción vegetativa. En investigaciones previas, realizadas por los autores, se seleccionaron cepas de Clonostachys y Trichoderma como potenciales agentes de biocontrol del moho gris. El objetivo fue evaluar la eficacia de hongos antagonistas en el control de B. cinerea en mini-estacas de E

  20. O comportamento de pés-mãe de Eucalyptus globulus Labill. sujeitos a diferentes níveis de azoto e potássio Influence of nitrogen and potassium on Eucalyptus globulus Labill. mother plants

    Directory of Open Access Journals (Sweden)

    H. M. Ribeiro

    2007-07-01

    Full Text Available Nos sistemas de propagação vegetativa de plantas o estado nutricional dos pés-mãe é determinante para o sucesso do enraizamento das estacas que estes originam. Como tal, a recente implementação, em Portugal, da técnica de propagação vegetativa de Eucalyptus globulus Labill. ssp. globulus por enraizamento de estacas caulinares, justifica plenamente o estudo da fertilização de pés-mãe desta espécie e o seu efeito no enraizamento das estacas. Assim, e com o objectivo de avaliar o efeito do azoto e do potássio no crescimento de pés-mãe de E. globulus e no enraizamento das estacas obtidas, foi instalado um ensaio de fertilização que decorreu durante um período de 2 anos. No ensaio utilizaram-se 2 clones desta espécie (HD161 e CN5 e testaram-se 6 modalidades de fertilização, correspondentes à combinação de 3 níveis de azoto (50, 100, 200 mg N L-1 e 2 de potássio (50 e 100 mg K L-1 na solução nutritiva utilizada. No primeiro ano de crescimento (1998 os pés-mãe foram sujeitos a uma poda de formação, tendo-se avaliado a biomassa recolhida. No segundo ano (1999, para além da biomassa recolhida, determinou-se também o número de estacas produzidas por pé-mãe e a capacidade de enraizamento dessas estacas. Com o delineamento experimental utilizado não se observaram, em todos os parâmetros avaliados, diferenças significativas entre os dois níveis de potássio, nem interacções significativas entre o potássio e os restantes factores em estudo. Relativamente ao azoto, a análise dos dados obtidos permitiu concluir que o aumento da concentração de azoto na solução nutritiva, de 50 para 200 mg L-1, originou aumentos significativos da biomassa recolhida nas podas, da produção de estacas e da percentagem de enraizamento das estacas, contrariando a ideia que o aumento da disponibilidade de azoto pode conduzir uma redução do enraizamento. Os resultados permitem assim concluir que o azoto é determinante para o

  1. Allelopathic effect of aqueous extracts of Eucalyptus globulus Labill. and of Casearia sylvestris Sw. on cropsEfeito alelopático de extratos aquosos de Eucalyptus globulus Labill. e de Casearia sylvestris Sw. sobre espécies cultivadas

    Directory of Open Access Journals (Sweden)

    Grasielle Soares Gusman

    2011-10-01

    Full Text Available Allelopathy is characterized by the harmful or benefic effects caused by secondary metabolites, that are produced by plants, microorganisms or fungi and are released in the environment, on the development of natural biological systems or implemented ones. This study aimed to evaluate the allelopathic effects of aqueous extracts of eucalypt (Eucalyptus globulus Labill. and wild coffee (Casearia sylvestris Sw. on the germination and initial development of mustard (Brassica campestris L., cabbage (Brassica oleracea L. cv. capitata, broccoli (Brassica oleracea L. cv. italica, kale (Brassica pekinensis L., lettuce (Lactuca sativa L. cv. grand rapids, tomato (Lycopersicum esculentum Miller, turnip (Brassica rapa L., rucola (Eruca sativa L. and radish (Raphanus sativus L.. Six concentrations of each aqueous extract were tested (10, 30, 50, 70, 90 and 100% and compared to control (distilled water, with five replicates of each concentration, being ten seeds of each crop distributed in each replicate. The aqueous extracts of E. globulus and C. sylvestris reduced significantly the percentage of seed germination, the index of germination speed and the initial growth of the above ground part and roots of all cultivated species, being the reduction of these parameters higher with the increment of the aqueous extracts concentration, which led to thicker and atrophied roots with a higher number of absorbent hairs. Therefore, the results indicate an existence of allelopathic potential of E. globulus and C. sylvestris.A alelopatia caracteriza-se pelos efeitos danosos ou benéficos que metabólitos secundários produzidos por plantas, microrganismos ou fungos liberados no ambiente exercem sobre o desenvolvimento de sistemas biológicos naturais ou implantados. O objetivo do trabalho foi avaliar o efeito alelopático de extratos aquosos de eucalipto (Eucalyptus globulus Labill. e guaçatonga (Casearia sylvestris Sw. na germinação e no crescimento inicial de

  2. Deposição de serapilheira e nutrientes em plantio de Eucalyptus urophylla × E. globulus

    Directory of Open Access Journals (Sweden)

    Márcio Viera

    Full Text Available Objetivou-se com o presente estudo avaliar a deposição de serapilheira e nutrientes em plantio de Eucalyptus urophylla × Eucalyptus globulus, em Eldorado do Sul-RS. Foram alocadas sistematicamente quatro parcelas de 20 m × 20 m, cada uma com quatro coletores de serapilheira de 1 m². A serapilheira foi coletada quinzenalmente, entre janeiro/2007 e dezembro/2010. A produção de serapilheira foi crescente, com o aumento da idade do povoamento, sendo 6,9 Mg ha-1 aos seis e 8,5 Mg ha-1 aos nove anos. A fração folhas correspondeu, em média, a 66,9% da serapilheira total, seguida pelos galhos finos (14,7%, miscelânea (10,5% e galhos grossos (7,9%. A deposição apresentou padrão sazonal, sendo maior no período de aumento da temperatura do ar. A fração folhas foi responsável por maior parte do retorno de nutrientes via serapilheira, variando de 58,2 a 81,8% da quantidade de Cu e N, respectivamente.

  3. Functional relationships between wood structure and vulnerability to xylem cavitation in races of Eucalyptus globulus differing in wood density.

    Science.gov (United States)

    Barotto, Antonio José; Monteoliva, Silvia; Gyenge, Javier; Martinez-Meier, Alejandro; Fernandez, María Elena

    2018-02-01

    Wood density can be considered as a measure of the internal wood structure, and it is usually used as a proxy measure of other mechanical and functional traits. Eucalyptus is one of the most important commercial forestry genera worldwide, but the relationship between wood density and vulnerability to cavitation in this genus has been little studied. The analysis is hampered by, among other things, its anatomical complexity, so it becomes necessary to address more complex techniques and analyses to elucidate the way in which the different anatomical elements are functionally integrated. In this study, vulnerability to cavitation in two races of Eucalyptus globulus Labill. with different wood density was evaluated through Path analysis, a multivariate method that allows evaluation of descriptive models of causal relationship between variables. A model relating anatomical variables with wood properties and functional parameters was proposed and tested. We found significant differences in wood basic density and vulnerability to cavitation between races. The main exogenous variables predicting vulnerability to cavitation were vessel hydraulic diameter and fibre wall fraction. Fibre wall fraction showed a direct impact on wood basic density and the slope of vulnerability curve, and an indirect and negative effect over the pressure imposing 50% of conductivity loss (P50) through them. Hydraulic diameter showed a direct negative effect on P50, but an indirect and positive influence over this variable through wood density on one hand, and through maximum hydraulic conductivity (ks max) and slope on the other. Our results highlight the complexity of the relationship between xylem efficiency and safety in species with solitary vessels such as Eucalyptus spp., with no evident compromise at the intraspecific level. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  4. Targeting oxidative stress, acetylcholinesterase, proinflammatory cytokine, dopamine and GABA by eucalyptus oil (Eucalyptus globulus) to alleviate ketamine-induced psychosis in rats.

    Science.gov (United States)

    Yadav, Monu; Jindal, Deepak Kumar; Parle, Milind; Kumar, Anil; Dhingra, Sameer

    2018-02-20

    Essential oil of eucalyptus species is among the most common traded essential oils in the world. There is an increasing interest in the application of eucalyptus oil as a natural additive in food and pharmaceutical industry. The present study was undertaken to identify the phytoconstituents present in the essential oil of Eucalyptus globulus leaves (EO) and ascertain their protective effect against ketamine-induced psychosis in rats. GC-MS technique was used for analysis of phytoconstituents present in EO. Ketamine (50 mg/kg, i.p.) was used to induce psychosis in rats. Photoactometer, forced swim test and pole climb avoidance test were used to evaluate the protective effects of the EO (500, 1000 and 2000 mg/kg, p.o.) on acute and chronic administration. Bar test was used to test the side effect of EO. Biochemical and neurochemical estimations were carried out to explore the possible mechanism of action. GC-MS analysis of EO showed the presence of a number of biologically active compounds. EO at the dose of 500, 1000 and 2000 mg/kg, p.o. on acute and chronic administration, decreased locomotor activity, immobility duration and latency to climb the pole. EO was effective to facilitate the release of GABA, increase GSH levels, inhibit dopamine neurotransmission and decrease TNF-α levels as well as diminish AChE activity in different regions of the brain. EO at the dose of 500, 1000 mg/kg did not produce cataleptic behavior in rats. EO at the dose of 500, 1000 mg/kg produced protective effects against ketamine-induced psychosis and can be further explored clinically against neuropsychiatric disorders.

  5. Studies on the antidandruff activity of the essential oil of coleus amboinicus and eucalyptus globulus

    Directory of Open Access Journals (Sweden)

    P.Selvakumar

    2012-10-01

    Full Text Available Objective: To determine the effect of the essential oil of Coleus amboinicus and Eucalyptus globules as potential antidandruff agent. Methods: Fresh leaves of Coleus amboinicus and Eucalyptus globules were collected from the Horticultural Research Station, Yercaud, Salem, Tamil Nadu, India. The collected leaves were washed thoroughly and shade dried for 7-10 days. The essential oils from both the dried plant leaves were obtained by hydro distillation procedure. The chemical compositions of hydro distilled essential oils were analyzed by Gas Chromatography-Mass spectrometry (GC-MS. The pure essential oils and the essential oils diluted with coconut oil of the two plants were used to check antidandruff activity against the dandruff causing fungus Malassezia furfur by agar well diffusion assay. Results: The average yield of essential oil from Coleus amboinicus (0.89% and Eucalyptus globules (0.77%. The results of GC-MS revealed that the presence of eight compounds from Coleus amboinicus essential oil and five constituents from Eucalyptus globules representing 90.45% and 96.32% of the total oil respectively. The major component was identified as Thymol (phenolic rich compocnd (41.3% in Coleus amboinicus and in Eucalyptus globules the major constituents was 1-8, Cineol (91.61%. In the antidandruff activity the broad range of inhibitory zone was observed in Eucalyptus globules (37mm oil compare to Coleus amboinicus (31mm. Ketaconazole based shampoo used as a standard. Conclusion: This study would direct to the establishment of a natural compound from Coleus amboinicus and Eucalyptus globules as an antidandruff agent which can be used for the production of potential antifungal drug and novel pharmaceutical and cosmeceutical leads.

  6. Efeito do teor de lignina da madeira de Eucalyptus globulus Labill. no desempenho da polpação kraft

    Directory of Open Access Journals (Sweden)

    Gabriel Valim Cardoso

    2011-01-01

    Full Text Available In this research, it was analyzed the lignin content effect of Eucalyptus globulus Labill. wood in kraft pulping optimization. Seventy-two laboratory cooking were made with wood chips obtained from six Eucalyptus globulus trees selected from a group of 50 trees. The wood chips from three trees with the lowest lignin content, with average 20.53%, were mixed proportionally based on the tree weights, obtaining the sample of low lignin content wood. The same was made to obtain the sample for wood chips with the highest lignin content, with average 23.02%. The two lignin levels were statistically different. The two wood samples had basic densities statistically not different. Using three maximum temperatures levels (160, 165 and 170ºC, and three active alkali charged (17, 18.5 and 20%, the wood chips were converted to kraft pulps. The pulps were then characterized to analyze the influence of the distinct treatments employed in the cooking on their properties. The effect of the cooking conditions was expressed by mathematical models in order to determine the optimum points for each of the evaluated properties. The optimization process indicated maximum temperature of 168ºC, and active alkali of 19%, for maximum kraft pulping yield to achieve kappa number 18; this result was for woods with low lignin content. For woods containing the high lignin content, the optimization showed maximum cooking temperature of 169ºC and active alkali of 19% for kappa number of 18. The average reduction of 2.49% in wood lignin content resulted a correspondent gain of 2.2% in the kraft yield (o.d. basis and a reduction on the active alkali charge of 1.2% (o.d. basis to achieve kappa numbers from 16 to 19, preserving pulp properties. If the option is to work with kappa number 19 instead of 16, the gain in kraft yield is approximately 2%. Therefore, when working with low lignin content wood and kappa number 19 instead of 16, a substantial gain of approximately 4.2% is

  7. MODELACIÓN DEL EFECTO DE LA PODA Y RALEO SOBRE LAS DEFORMACIONES RESIDUALES LONGITUDINALES EN Eucalyptus globulus Labill.

    Directory of Open Access Journals (Sweden)

    Eduardo Navarrete

    2016-01-01

    Full Text Available RESUMEN Se estudió una plantación de Eucalyptus globulus ubicada en el sector precordillerano andino de la región del Bío Bío, Chile, c on el objetivo de evaluar y modelar el efecto de la poda y raleo sobre las deformaciones residuales longitudinales (DRL . El lugar de estudio correspondió a un rodal de 12 años de edad, el cual fue intervenido a los 2 años y medio de establecido, donde se evaluaron distintas intensidades de poda (0% y 60% de la altura total y densidades de raleo (1.600 árb·ha -1 (sin raleo y 800 árb·ha -1 . En cada tratamiento definido por la combinación de los dos niveles de poda y raleo, se estudió además, el efecto de la clase de copa (dominante, codominante e intermedia sobre el comportamiento de las DRL. En cada unidad de muestreo se registraron variables a nivel de árbol individual, estimando los parámetros de rodal más relevantes. Se muestreó un total de 36 árboles, midiéndose en cada uno de ellos las DRL a nivel del Dap con un extensómetro diseñado por el CIRAD-Forêt. Los resultados determinaron que la poda y raleo no afectaron significativamente el comportamiento de las DRL en Eucalyptus globulus ; en cambio, la clase de copa, tuvo un efecto altamente significativo sobre la variación de las deformaciones residuales, presentando los árboles dominantes mayores niveles de deformación que las clases de copa codominante e intermedia, respectivamente. El modelo de estimación incluyó como variables predictivas del comportamiento de las DRL , las expresiones logarítmicas del Dap y del inverso multiplicativo de la varianza de los radios de copa, además de la poda, presentando un R 2 de 0,44 y un EEE de 0,132.

  8. Monoterpene engineering in a woody plant Eucalyptus camaldulensis using a limonene synthase cDNA.

    Science.gov (United States)

    Ohara, Kazuaki; Matsunaga, Etsuko; Nanto, Kazuya; Yamamoto, Kyoko; Sasaki, Kanako; Ebinuma, Hiroyasu; Yazaki, Kazufumi

    2010-01-01

    Metabolic engineering aimed at monoterpene production has become an intensive research topic in recent years, although most studies have been limited to herbal plants including model plants such as Arabidopsis. The genus Eucalyptus includes commercially important woody plants in terms of essential oil production and the pulp industry. This study attempted to modify the production of monoterpenes, which are major components of Eucalyptus essential oil, by introducing two expression constructs containing Perilla frutescens limonene synthase (PFLS) cDNA, whose gene products were designed to be localized in either the plastid or cytosol, into Eucalyptus camaldulensis. The expression of the plastid-type and cytosol-type PFLS cDNA in transgenic E. camaldulensis was confirmed by real-time polymerase chain reaction (PCR). Gas chromatography with a flame ionization detector analyses of leaf extracts revealed that the plastidic and cytosolic expression of PFLS yielded 2.6- and 4.5-times more limonene than that accumulated in wild-type E. camaldulensis, respectively, while the ectopic expression of PFLS had only a small effect on the emission of limonene from the leaves of E. camaldulensis. Surprisingly, the high level of PFLS in Eucalyptus was accompanied by a synergistic increase in the production of 1,8-cineole and alpha-pinene, two major components of Eucalyptus monoterpenes. This genetic engineering of monoterpenes demonstrated a new potential for molecular breeding in woody plants.

  9. Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region

    Directory of Open Access Journals (Sweden)

    Paula eGuzmán

    2014-09-01

    Full Text Available The plant cuticle has traditionally been conceived as an independent hydrophobic layer that covers the external epidermal cell wall. Due to its complexity, the existing relationship between cuticle chemical composition and ultra-structure remains unclear to date. This study aimed to examine the link between chemical composition and structure of isolated, adaxial leaf cuticles of Eucalyptus camaldulensis and E. globulus by the gradual extraction and identification of lipid constituents (cutin and soluble lipids, coupled to spectroscopic and microscopic analyses. The soluble compounds and cutin monomers identified could not be assigned to a concrete internal cuticle ultra-structure. After cutin depolymerization, a cellulose network resembling the cell wall was observed, with different structural patterns in the regions ascribed to the cuticle proper and cuticular layer, respectively. Our results suggest that the current cuticle model should be revised, stressing the presence and major role of cell wall polysaccharides. It is concluded that the cuticle may be interpreted as a modified cell wall region which contains additional lipids. The major heterogeneity of the plant cuticle makes it difficult to establish a direct link between cuticle chemistry and structure with the existing methodologies.

  10. Atividade ovicida e larvicida in vitro do óleo essencial de Eucalyptus globulus sobre Haemonchus contortus Ovicidal and larvicidal activity in vitro of Eucalyptus globulus essential oils on Haemonchus contortus

    Directory of Open Access Journals (Sweden)

    Iara T. F. Macedo

    2009-09-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito do óleo essencial de Eucalyptus globulus (OEEG sobre a eclosão e desenvolvimento de larvas de Haemonchus contortus. A determinação da composição química do OEEG foi feita por meio de cromatografia gasosa acoplada à espectrometria de massa. No teste de eclosão de ovos (TEO, utilizaram-se as concentrações 21,75; 17,4; 8,7; 5,43 e 2,71 mg.mL-1; e no de desenvolvimento larvar (TDL as concentrações foram 43,5; 21,75; 10,87; 5,43 e 2,71 mg.mL-1. Cada ensaio foi acompanhado por um controle positivo, 0,02 mg.mL-1 tiabendazol, no TEO e 0,008 mg.mL-1 ivermectina no TDL, e por um controle negativo com o diluente Tween 80 (3%. A eficácia máxima obtida pelo OEEG sobre ovos foi de 99,3% na concentração de 21,75 mg.mL-1 e, sobre larvas, 98,7% na concentração de 43,5 mg.mL-1. A concentração do OEEG, que inviabilizou 50% dos ovos e das larvas do parasito, foi de 8,3 e 6,92 mg.mL-1, respectivamente. A análise química do óleo identificou como principal componente o monoterpeno 1,8-cineol. O OEEG apresentou atividade ovicida e larvicida in vitro sobre H. contortus, determinando um bom potencial para utilização no controle de nematóides gastrintestinais de ovinos e caprinos.The objective of this work was to evaluate ovicidal and larvicidal effects of Eucalyptus globulus essential oil (EGEO on Haemonchus contortus. The chemical composition determination of EGEO was through gas chromatography and mass spectrometry. Egg hatch test (EHT was performed in concentrations 21.75; 17.4; 8.7; 5.43 e 2.71 mg.mL-1. In larval development test (LDT were used the concentrations 43.5; 21.75; 10.87; 5.43 e 2.71 mg.mL-1. Each trial was conducted by negative control with Tween 80 (3% and positive control, 0.02 mg.mL -1 of thiabendazole in EHT and 0.008 mg.mL-1 of ivermectin in LDT. The maximum effectiveness of EGEO on eggs was 99.3% in concentration of 21.75 mg.mL-1 and on larvae was 98.7% in concentration 43

  11. Fungi associated to bark lesions of Eucalyptus globulus stems in plantations from Uruguay Fungos asociados as lesões da casca do caule de Eucalyptus globulus em plantações no uruguai

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    Raquel Alonso

    2009-08-01

    Full Text Available Trees with stem bark lesions are frequently observed in Eucalyptus globulus Labill. plantations, particularly in the central west region of Uruguay. These lesions constitute a problem for trunk decortications at harvest and they also facilitate the access of fungi that could cause wood decay. Seven, three and oneyear-old plantations, located at three sites in close proximity were selected. Four types of trunk lesions were present in trees regardless the age of plantation and more than one type was found in each plantation. The aim of this study was to investigate the fungal composition associated with these lesions and compare them to healthy tissues and try to find out the origin of these symptoms. Another purpose was to elucidate the real role of the fungi considered pathogens by means of experimental inoculations. Segments from lesions and healthy tissues yielded 897 fungal isolates belonging to 32 taxa, 681 isolates from bark lesions and 216 from healthy tissues. Both healthy and symptomatic tissues showed similar fungal species composition, but with differences in frequencies of colonization. Cytospora eucalypticola Van der Westhuizen, Botryosphaeria spp., Pestalotiopsis guepinii (Desm. Stey. and Penicillium spp. were the dominant species isolated. As symptoms were not reproduced after experimental inoculation with Botryosphaeria ribis Grossenb. & Duggar and B. eucalyptorum Crous, & M.J. Wingf, it could be suggested that these lesions were originated by unfavorable environmental conditions. The frost that occurred for several days out of season and flooding may have been involved in the development of bark lesion.As lesões na casca de Eucalyptus globulus Labill. são frequentemente observadas nas plantações da Região Centro-Oeste do Uruguai. Constituem problema para o descortiçamento na colheita e, além disso, facilita a penetraçao de fungos apodrecedores da madeira. Selecionaram-se plantações com 1, 3 e 7 anos de idade, em tr

  12. In vitro effect of Eucalyptus globulus essential oil on Penicillium sp.Efeito in vitro do óleo essencial de Eucalyptus globulus sobre o crescimento e desenvolvimento de Penicillium sp.

    Directory of Open Access Journals (Sweden)

    Marcia Holanda Nozaki

    2011-08-01

    Full Text Available Penicillium sp. is the casual agent of moulds, considered the main citrus post-harvest disease, causing loss on the quality and quantity of marketable fruits. Besides economic loss, it can be included costs, transportation and fruit storage problems. The present work had the objective to verify the fungitoxic action of Eucalyptus globules essential oil on the in vitro control of Penicillium sp., evaluating mycelial growth, production and spores germination. The experiment was conduced on the Microbiology laboratory of PUC, Toledo campus, with eight treatments: 1%, 0,5%, 0,25%, 0,1%, 0,05% e 0,025% concentrations of eucalyptus oil added to PDA media (potato-dextrose-agar, negative control (PDA with addition of 40mg of azoxystrobin/L and positive control (PDA media only. The experimental design was according to a completely randomized design, with five replicates each treatment. The eucalyptus essential oil inhibited significantly the growth in the 1%, 0,5% e 0,25% concentrations, not differing statistically from control with fungicide azoxystrobin. The treatments presented the same behavior for spores production. For spores germination, the 1% e 0,5% concentrations had better control than treatment with azoxystrobin and the concentration of 0,25%. Others oil concentrations did not present significative results on the evaluations, being statistically equal to positive control (PDA.O fungo Penicillium sp. é o agente causal dos bolores, considerada a principal doença pós-colheita em citros, levando à perdas na qualidade e quantidade dos frutos comercializáveis. Além de perdas econômicas, uma vez que nas perdas pós-colheitas estão inclusos os custos, transporte e armazenagem de frutos. O presente trabalho teve por objetivo verificar a ação fungitóxica do óleo essencial de Eucalyptus globulus no controle in vitro de Penicillium sp., avaliando seu crescimento micelial, produção e a germinação de esporos. O experimento foi conduzido no

  13. Chemical characteristics and Kraft pulping of tension wood from Eucalyptus globulus labill Características químicas e polpação Kraft de madeira de tração de Eucalyptus globulus labill

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    María Graciela Aguayo

    2012-12-01

    Full Text Available Tension (TW and opposite wood (OW of Eucalyptus globulus trees were analyzed for its chemical characteristics and Kraft pulp production. Lignin content was 16% lower and contained 32% more syringyl units in TW than in OW. The increase in syringyl units favoured the formation of β-O-4 bonds that was also higher in TW than in OW (84% vs. 64%, respectively. The effect of these wood features was evaluated in the production of Kraft pulps from both types of wood. At kappa number 16, Kraft pulps obtained from TW demanded less active alkali in delignification and presented slightly higher or similar pulp yield than pulps made with OW. Fiber length, coarseness and intrinsic viscosity were also higher in tension than in opposite pulps. When pulps where refined to 30°SR, TW pulps needed 18% more revolutions in the PFI mill to achieve the same beating degree than OW pulps. Strength properties (tensile, tear and burst indexes were slightly higher or similar in tension as compared with opposite wood pulps. After an OD0(EOD1 bleaching sequence, both pulps achieved up to 89% ISO brightness. Bleached pulps from TW presented higher viscosity and low amount of hexenuronic acids than pulps from OW. Results showed that TW presented high xylans and low lignin content that caused a decrease in alkali consumption, increase pulp strength properties and similar bleaching performance as compared with pulps from OW.Madeira de tração e oposta de árvores de Eucalyptus globulus foram analisadas quanto a suas características químicas e produção de polpa Kraft. A caracterização química da madeira de tração (TW de Eucalyptus globulus Labill. mostrou um conteúdo similar de celulose, alto conteúdo de xilanas e baixo conteúdo de lignina quando comparada com a madeira oposta (OW de uma mesma árvore. O conteúdo de lignina foi 16% menor e contém 32% mais unidades siringila em TW que em OW. O aumento das unidades siringila favoreceu a formação de ligações

  14. Dust loadings on some common plants near Lucknow city. [Acacia melanoxylon, Bauhinia malabarica, Bougain-villea glabra, Calotropis procera, Catharanthus roseus, Eucalyptus globulus, Ipomoea fistulosa and Peltophorum pterocarpum

    Energy Technology Data Exchange (ETDEWEB)

    Yunus, M.; Dwivedi, A.K.; Kulshreshtha, K.; Ahmad, K.J.

    1985-01-01

    Eight plant species - Acacia melanoxylon, Bauhinia malabarica, Bougain-villea glabra, Calotropis procera, Catharanthus roseus, Eucalyptus globulus, Ipomoea fistulosa and Peltophorum pterocarpum - were collected from a newly established suburb colony of Lucknow city, where the major pollutant is dust, to study the dust cleansing efficiency of the plant canopy and also to establish the correlation between the leaf morphological characteristics and their dust trapping potential. The dust load, in milligrams per square centimeter of leaf surface, was measured and related to foliar epidermal and cuticular characteristics, and morphological features.

  15. Modelling stand biomass fractions in Galician Eucalyptus globulus plantations by use of different LiDAR pulse densities

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez-Ferreiro, E.; Miranda, D.; Barreiro-Fernandez, L.; Bujan, S.; Garcia-Gutierrez, J.; Dieguez-Aranda, U.

    2013-07-01

    Aims of study: To evaluate the potential use of canopy height and intensity distributions, determined by airborne LiDAR, for the estimation of crown, stem and aboveground biomass fractions. To assess the effects of a reduction in LiDAR pulse densities on model precision. Area of study: The study area is located in Galicia, NW Spain. The forests are representative of Eucalyptus globulus stands in NW Spain, characterized by low-intensity silvicultural treatments and by the presence of tall shrub. Material and methods: Linear, multiplicative power and exponential models were used to establish empirical relationships between field measurements and LiDAR metrics. A random selection of LiDAR returns and a comparison of the prediction errors by LiDAR pulse density factor were performed to study a possible loss of fit in these models. Main results: Models showed similar goodness-of-fit statistics to those reported in the international literature. R2 ranged from 0.52 to 0.75 for stand crown biomass, from 0.64 to 0.87 for stand stem biomass, and from 0.63 to 0.86 for stand aboveground biomass. The RMSE/MEAN 100 of the set of fitted models ranged from 17.4% to 28.4%. Models precision was essentially maintained when 87.5% of the original point cloud was reduced, i.e. a reduction from 4 pulses m{sup 2} to 0.5 pulses m{sup 2}. Research highlights: Considering the results of this study, the low-density LiDAR data that are released by the Spanish National Geographic Institute will be an excellent source of information for reducing the cost of forest inventories. (Author)

  16. Inhibition of growth and biofilm formation of clinical bacterial isolates by NiO nanoparticles synthesized from Eucalyptus globulus plants.

    Science.gov (United States)

    Saleem, Samia; Ahmed, Bilal; Khan, Mohammad Saghir; Al-Shaeri, Majed; Musarrat, Javed

    2017-10-01

    Nanotechnology based therapeutics has emerged as a promising approach for augmenting the activity of existing antimicrobials due to the unique physical and chemical properties of nanoparticles (NPs). Nickel oxide nanoparticles (NiO-NPs) have been suggested as prospective antibacterial and antitumor agent. In this study, NiO-NPs have been synthesized by a green approach using Eucalyptus globulus leaf extract and assessed for their bactericidal activity. The morphology and purity of synthesized NiO-NPs determined through various spectroscopic techniques like UV-Visible, FT-IR, XRD, EDX and electron microscopy differed considerably. The synthesized NiO-NPs were pleomorphic varying in size between 10 and 20 nm. The XRD analysis revealed the average size of NiO-NPs as 19 nm. The UV-Vis spectroscopic data showed a strong SPR of NiO-NPs with a characteristic spectral peak at 396 nm. The FTIR data revealed various functional moieties like C=C, C-N, C-H and O-H which elucidate the role of leaf biomolecules in capping and dispersal of NiO-NPs. The bioactivity assay revealed the antibacterial and anti-biofilm activity of NiO-NPs against ESβL (+) E. coli, P. aeruginosa, methicillin sensitive and resistant S. aureus. Growth inhibition assay demonstrated time and NiO-NPs concentration dependent decrease in the viability of treated cells. NiO-NPs induced biofilm inhibition was revealed by a sharp increase in characteristic red fluorescence of PI, while SEM images of NiO-NPs treated cells were irregular shrink and distorted with obvious depressions/indentations. The results suggested significant antibacterial and antibiofilm activity of NiO-NPs which may play an important role in the management of infectious diseases affecting human health. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Concerted transcription of auxin and carbohydrate homeostasis-related genes underlies improved adventitious rooting of microcuttings derived from far-red treated Eucalyptus globulus Labill mother plants.

    Science.gov (United States)

    Ruedell, Carolina Michels; de Almeida, Márcia Rodrigues; Fett-Neto, Arthur Germano

    2015-12-01

    Economically important plant species, such as Eucalyptus globulus, are often rooting recalcitrant. We have previously shown that far-red light enrichment applied to E. globulus donor-plants improved microcutting rooting competence and increased rooting zone/shoot carbohydrate ratio. To better understand this developmental response, the relative expression profiles of genes involved in auxin signaling (ARF6, ARF8, AGO1), biosynthesis (YUC3) and transport (AUX1, PIN1, PIN2); sucrose cleavage (SUS1, CWINV1), transport (SUC5), hexose phosphorylation (HXK1, FLN1) and starch biosynthesis (SS3) were quantified during adventitious rooting of E. globulus microcuttings derived from donor plants exposed to far-red or white light. Expression of auxin transport-related genes increased in the first days of root induction. Far-red enrichment of donor plants induced ARF6, ARF8 and AGO1 in microcuttings. The first two gene products could activate GH3 and other rooting related genes, whereas AGO1 deregulation of the repressor ARF17 may relief rooting inhibition. Increased sink strength at the basal stem with sucrose unloading in root tissue mediated by SUC and subsequent hydrolysis by SUS1 were also supported by gene expression profile. Fructose phosphorylation and starch biosynthesis could also contribute to proper carbon allocation at the site of rooting, as evidenced by increased expression of related genes. These data are in good agreement with increased contents of hexoses and starch at the cutting base severed from far-red exposed donor plants. To sum up, pathways integrating auxin and carbohydrate metabolism were activated in microcuttings derived from donor plants exposed to far red light enrichment, thereby improving rooting response in E. globulus. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  18. Combining autohydrolysis and ionic liquid microwave treatment to enhance enzymatic hydrolysis of Eucalyptus globulus wood.

    Science.gov (United States)

    Rigual, Victoria; Santos, Tamara M; Domínguez, Juan Carlos; Alonso, M Virginia; Oliet, Mercedes; Rodriguez, Francisco

    2018-03-01

    The combination of autohydrolysis and ionic liquid microwave treatments of eucalyptus wood have been studied to facilitate sugar production in a subsequent enzymatic hydrolysis step. Three autohydrolysis conditions (150 °C, 175 °C and 200 °C) in combination with two ionic liquid temperatures (80 °C and 120 °C) were compared in terms of chemical composition, enzymatic digestibility and sugar production. Morphology was measured (using SEM) and the biomass surface was visualized with confocal fluorescence microscopy. The synergistic cooperation of both treatments was demonstrated, enhancing cellulose accessibility. At intermediate autohydrolysis conditions (175 °C) and low ionic liquid temperature (80 °C), a glucan digestibility of 84.4% was obtained. Using SEM micrographs, fractal dimension (as a measure of biomass complexity) and lacunarity (as a measure of homogeneity) were calculated before and after pretreatment. High fractals dimensions and low lacunarities correspond to morphologically complex and homogeneous samples, that are better digested by enzyme cocktails. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Reference gene selection for quantitative reverse transcription-polymerase chain reaction normalization during in vitro adventitious rooting in Eucalyptus globulus Labill.

    Science.gov (United States)

    de Almeida, Márcia R; Ruedell, Carolina M; Ricachenevsky, Felipe K; Sperotto, Raul A; Pasquali, Giancarlo; Fett-Neto, Arthur G

    2010-09-20

    Eucalyptus globulus and its hybrids are very important for the cellulose and paper industry mainly due to their low lignin content and frost resistance. However, rooting of cuttings of this species is recalcitrant and exogenous auxin application is often necessary for good root development. To date one of the most accurate methods available for gene expression analysis is quantitative reverse transcription-polymerase chain reaction (qPCR); however, reliable use of this technique requires reference genes for normalization. There is no single reference gene that can be regarded as universal for all experiments and biological materials. Thus, the identification of reliable reference genes must be done for every species and experimental approach. The present study aimed at identifying suitable control genes for normalization of gene expression associated with adventitious rooting in E. globulus microcuttings. By the use of two distinct algorithms, geNorm and NormFinder, we have assessed gene expression stability of eleven candidate reference genes in E. globulus: 18S, ACT2, EF2, EUC12, H2B, IDH, SAND, TIP41, TUA, UBI and 33380. The candidate reference genes were evaluated in microccuttings rooted in vitro, in presence or absence of auxin, along six time-points spanning the process of adventitious rooting. Overall, the stability profiles of these genes determined with each one of the algorithms were very similar. Slight differences were observed in the most stable pair of genes indicated by each program: IDH and SAND for geNorm, and H2B and TUA for NormFinder. Both programs identified UBI and 18S as the most variable genes. To validate these results and select the most suitable reference genes, the expression profile of the ARGONAUTE1 gene was evaluated in relation to the most stable candidate genes indicated by each algorithm. Our study showed that expression stability varied between putative reference genes tested in E. globulus. Based on the AGO1 relative expression

  20. Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production

    Directory of Open Access Journals (Sweden)

    Carlos M. Silva

    2012-06-01

    Full Text Available Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids. In this work, the supercritical fluid extraction (SFE of Eucalyptus globulus deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100–200 bar, co-solvent (ethanol content (0, 5 and 8% wt, and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by several tens of bar. At 160 bar and 40 °C, the introduction of 8% (wt of ethanol greatly improves the yield of triterpenoids more than threefold.

  1. Above-ground biomass production and allometric relations of Eucalyptus globulus Labill. coppice plantations along a chronosequence in the central highlands of Ethiopia

    International Nuclear Information System (INIS)

    Zewdie, Mulugeta; Olsson, Mats; Verwijst, Theo

    2009-01-01

    Eucalyptus plantations are extensively managed for wood production in the central highlands of Ethiopia. Nevertheless, little is known about their biomass (dry matter) production, partitioning and dynamics over time. Data from 10 different Eucalyptus globulus stands, with a plantation age ranging from 11 to 60 years and with a coppice-shoot age ranging from 1 to 9 years were collected and analyzed. Above-ground tree biomass of 7-10 sampled trees per stand was determined destructively. Dry weights of tree components (W c ; leaves, twigs, branches, stembark, and stemwood) and total above-ground biomass (W a ) were estimated as a function of diameter above stump (D), tree height (H) and a combination of these. The best fits were obtained, using combinations of D and H. When only one explanatory variable was used, D performed better than H. Total above-ground biomass was linearly related to coppice-shoot age. In contrast a negative relation was observed between the above-ground biomass production and total plantation age (number of cutting cycles). Total above-ground biomass increased from 11 t ha -1 at a stand age of 1 year to 153 t ha -1 at 9 years. The highest dry weight was allocated to stemwood and decreased in the following order: stemwood > leaves > stembark > twigs > branches. The equations developed in this study to estimate biomass components can be applied to other Eucalyptus plantations under the assumption that the populations being studied are similar with regard to density and tree size to those for which the relationships were developed

  2. Above-ground biomass production and allometric relations of Eucalyptus globulus Labill. coppice plantations along a chronosequence in the central highlands of Ethiopia

    Energy Technology Data Exchange (ETDEWEB)

    Zewdie, Mulugeta; Olsson, Mats; Verwijst, Theo [Swedish University of Agricultural Sciences, Department of Crop Production Ecology, P.O. Box 7043, 75007 Uppsala (Sweden)

    2009-03-15

    Eucalyptus plantations are extensively managed for wood production in the central highlands of Ethiopia. Nevertheless, little is known about their biomass (dry matter) production, partitioning and dynamics over time. Data from 10 different Eucalyptus globulus stands, with a plantation age ranging from 11 to 60 years and with a coppice-shoot age ranging from 1 to 9 years were collected and analyzed. Above-ground tree biomass of 7-10 sampled trees per stand was determined destructively. Dry weights of tree components (W{sub c}; leaves, twigs, branches, stembark, and stemwood) and total above-ground biomass (W{sub a}) were estimated as a function of diameter above stump (D), tree height (H) and a combination of these. The best fits were obtained, using combinations of D and H. When only one explanatory variable was used, D performed better than H. Total above-ground biomass was linearly related to coppice-shoot age. In contrast a negative relation was observed between the above-ground biomass production and total plantation age (number of cutting cycles). Total above-ground biomass increased from 11 t ha{sup -1} at a stand age of 1 year to 153 t ha{sup -1} at 9 years. The highest dry weight was allocated to stemwood and decreased in the following order: stemwood > leaves > stembark > twigs > branches. The equations developed in this study to estimate biomass components can be applied to other Eucalyptus plantations under the assumption that the populations being studied are similar with regard to density and tree size to those for which the relationships were developed. (author)

  3. VARIABILIDAD Y RELACION ENTRE LAS PROPIEDADES ANATOMICAS, QUIMICAS Y LA APTITUD PULPABLE, EN CLONES DE EUCALYPTUS GLOBULUS.

    OpenAIRE

    RAMIREZ VIDAL, MAURICIO HERNAN; RAMIREZ VIDAL, MAURICIO HERNAN

    2008-01-01

    Se estudió la variación de la anatomía de la madera, variables biornétricas, aptitud pulpable y propiedades de la pulpa, en 14 clones de E. globulus de 7 años de edad, creciendo en un ensayo clonal en la zona de Arauco, región del Bio-Bio, Chile. Las Cara 61p.

  4. Impacts of population structure and analytical models in genome-wide association studies of complex traits in forest trees: a case study in Eucalyptus globulus.

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    Eduardo P Cappa

    Full Text Available The promise of association genetics to identify genes or genomic regions controlling complex traits has generated a flurry of interest. Such phenotype-genotype associations could be useful to accelerate tree breeding cycles, increase precision and selection intensity for late expressing, low heritability traits. However, the prospects of association genetics in highly heterozygous undomesticated forest trees can be severely impacted by the presence of cryptic population and pedigree structure. To investigate how to better account for this, we compared the GLM and five combinations of the Unified Mixed Model ( UMM on data of a low-density genome-wide association study for growth and wood property traits carried out in a Eucalyptus globulus population (n = 303 with 7,680 Diversity Array Technology (DArT markers. Model comparisons were based on the degree of deviation from the uniform distribution and estimates of the mean square differences between the observed and expected p-values of all significant marker-trait associations detected. Our analysis revealed the presence of population and family structure. There was not a single best model for all traits. Striking differences in detection power and accuracy were observed among the different models especially when population structure was not accounted for. The UMM method was the best and produced superior results when compared to GLM for all traits. Following stringent correction for false discoveries, 18 marker-trait associations were detected, 16 for tree diameter growth and two for lignin monomer composition (S:G ratio, a key wood property trait. The two DArT markers associated with S:G ratio on chromosome 10, physically map within 1 Mbp of the ferulate 5-hydroxylase (F5H gene, providing a putative independent validation of this marker-trait association. This study details the merit of collectively integrate population structure and relatedness in association analyses in undomesticated, highly

  5. Impacts of Population Structure and Analytical Models in Genome-Wide Association Studies of Complex Traits in Forest Trees: A Case Study in Eucalyptus globulus

    Science.gov (United States)

    Garcia, Martín N.; Acuña, Cintia; Borralho, Nuno M. G.; Grattapaglia, Dario; Marcucci Poltri, Susana N.

    2013-01-01

    The promise of association genetics to identify genes or genomic regions controlling complex traits has generated a flurry of interest. Such phenotype-genotype associations could be useful to accelerate tree breeding cycles, increase precision and selection intensity for late expressing, low heritability traits. However, the prospects of association genetics in highly heterozygous undomesticated forest trees can be severely impacted by the presence of cryptic population and pedigree structure. To investigate how to better account for this, we compared the GLM and five combinations of the Unified Mixed Model (UMM) on data of a low-density genome-wide association study for growth and wood property traits carried out in a Eucalyptus globulus population (n = 303) with 7,680 Diversity Array Technology (DArT) markers. Model comparisons were based on the degree of deviation from the uniform distribution and estimates of the mean square differences between the observed and expected p-values of all significant marker-trait associations detected. Our analysis revealed the presence of population and family structure. There was not a single best model for all traits. Striking differences in detection power and accuracy were observed among the different models especially when population structure was not accounted for. The UMM method was the best and produced superior results when compared to GLM for all traits. Following stringent correction for false discoveries, 18 marker-trait associations were detected, 16 for tree diameter growth and two for lignin monomer composition (S∶G ratio), a key wood property trait. The two DArT markers associated with S∶G ratio on chromosome 10, physically map within 1 Mbp of the ferulate 5-hydroxylase (F5H) gene, providing a putative independent validation of this marker-trait association. This study details the merit of collectively integrate population structure and relatedness in association analyses in undomesticated, highly

  6. Effect of the activity of the Brazilian polyherbal formulation: Eucalyptus globulus Labill, Peltodon radicans Pohl and Schinus terebinthifolius Radd in inflammatory models

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    Karina C.P. Medeiros

    Full Text Available The Brazilian polyherbal formulation (BPF is composed by dyes of Eucalyptus globulus Labill, Peltodon radicans Pohl and Schinus terebinthifolius Raddi in alcohol at 13.3° GL. The formulation is popularly used in Paraíba state, Brazil since 1889 and it is used as an antiseptic and anti-inflammatory medicine. The aim of this study was to evaluate the anti-inflammatory property of the polyherbal formulation. For this purpose it was used the12-O-tetradecanoylphorbol 13-acetate (TPA and capsaicin-induced mouse ear edema and the carrageenan-induced rat paw edema. The BPF at dose of 26 mL/Kg inhibited both 12-O-tetradecanoylphorbol 13-acetate (TPA and capsaicin-induced ear edema by 49% (p < 0.05 and 24% (p < 0.01 respectively. Preliminary results on carrageenan-induced rat paw edema demonstrated that oral administration also inhibited the paw edema by approximately 29%. The results demonstrate that the Brazilian polyherbal formulation has anti-inflammatory activity and the better dose was the one used by the population.

  7. A Biorefinery Cascade Conversion of Hemicellulose-Free Eucalyptus Globulus Wood: Production of Concentrated Levulinic Acid Solutions for γ-Valerolactone Sustainable Preparation

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    Sandra Rivas

    2018-04-01

    Full Text Available Eucalyptus globulus wood samples were subjected to preliminary aqueous processing to remove water-soluble extractives and hemicelluloses, and the resulting solid (mainly made up of cellulose and lignin was employed as a substrate for converting the cellulosic fraction into mixtures of levulinic and formic acid through a sulfuric acid-catalyzed reaction. These runs were carried out in a microwave-heated reactor at different temperatures and reaction times, operating in single-batch or cross-flow modes, in order to identify the most favorable operational conditions. Selected liquid phases deriving from these experiments, which resulted in concentrated levulinic acid up to 408 mmol/L, were then employed for γ-valerolactone production by levulinc acid hydrogenation in the presence of the commercial 5% Ru/C catalyst. In order to assess the effects of the main reaction parameters, hydrogenation experiments were performed at different temperatures, reaction times, amounts of ruthenium catalyst and hydrogen pressure. Yields of γ-valerolactone in the range of 85–90 mol % were obtained from the hydrogenation of the wood-derived solutions containing levulinic acid, obtained by single-batch operation or by the cross-flow process. The negative effect of co-produced formic acid present in crude levulinic acid solutions was evidenced and counteracted efficiently by allowing the preliminary thermal decomposition of formic acid itself.

  8. ESTIMATIVA DA BIOMASSA E DO CONTEÚDO DE NUTRIENTES DE UM POVOAMENTO DE Eucalyptus globulus (Labillardière SUB-ESPÉCIE maidenii

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    Mauro Valdir Schumacher

    2000-12-01

    Full Text Available O presente trabalho teve por objetivo estimar a produção de biomassa acima do solo e determinar o conteúdo de nutrientes nos diferentes componentes das árvores de um povoamento de Eucalyptus globulus subespécie maidenii. Os dados foram coletados em um talhão de 4 anos de idade, plantado em solo argiloso, no município de Butiá, Rio Grande do Sul. Para determinar a quantidade de biomassa, foi utilizada uma equação de regressão (lnY = a + b * lnX cujos coeficientes foram calculados com base em nove árvores amostradas em diferentes classes diamétricas. A biomassa total acima do solo foi de 83,2 Mg ha-1, em que 13; 10; 9 e 68 % encontravam-se distribuídos nas folhas, ramos, casca e madeira, respectivamente. Do total dos elementos na árvore, na madeira do tronco foram encontrados 29; 29; 40; 12,5 e 34% de N, P, K, Ca e Mg respectivamente. Já na copa das árvores, esses mesmos elementos representaram 64; 56; 48,5; 32 e 39%. A casca foi o componente que acumulou as maiores quantidades de cálcio, (55,3% do total.

  9. Reactivity of syringyl and guaiacyl lignin units and delignification kinetics in the kraft pulping of Eucalyptus globulus wood using Py-GC-MS/FID.

    Science.gov (United States)

    Lourenço, Ana; Gominho, Jorge; Marques, António Velez; Pereira, Helena

    2012-11-01

    Eucalyptus globulus sapwood and heartwood showed no differences in lignin content (23.0% vs. 23.7%) and composition: syringyl-lignin (17.9% vs. 18.0%) and guaiacyl-lignin (4.8% vs. 5.2%). Delignification kinetics of S- and G-units in heartwood and sapwood was investigated by Py-GC-MS/FID at 130, 150 and 170°C and modeled as double first-order reactions. Reactivity differences between S and G-units were small during the main pulping phase and the higher reactivity of S over G units was better expressed in the later pulping stage. The residual lignin composition in pulps was different from wood or from samples in the initial delignification stages, with more G and H-units. S/G ratio ranged from 3 to 4.5 when pulp residual lignin was higher than 10%, decreasing rapidly to less than 1. The S/H was initially around 20 (until 15% residual lignin), decreasing to 4 when residual lignin was about 3%. Copyright © 2012 Elsevier Ltd. All rights reserved.

  10. Ensaios clínicos toxicológicos, fase I, de um fitoterápico composto (Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour e Eucalyptus globulus Labill

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    Patrícia Trindade C. Paulo

    Full Text Available Neste estudo, foram realizados ensaios clínicos toxicológicos, fase I, do produto fitoterápico composto pelas plantas medicinais Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour e Eucalyptus globulus Labill. O estudo foi realizado no Hospital Universitário Lauro Wanderley/UFPB/PB e, para isto, foram selecionados 28 voluntários sadios, sendo 14 homens e 14 mulheres que ingeriram por via oral, ininterruptamente durante 8 semanas, 15 mL do produto, três vezes ao dia; e no 3º e 7º dia, 3ª e 6ª semanas e 24 h após a 8ª semana, foram feitas avaliações clínicas e laboratoriais para análise da toxicidade aguda e crônica. Os resultados obtidos demonstraram que os pacientes não apresentaram alterações clínicas, laboratoriais e reações adversas significantes, apenas pequenas alterações foram detectadas no sangue através da aspartato transaminase (AST e fosfatase alcalina no grupo feminino para um p < 0,05; no entanto, estes valores determinados permaneceram dentro dos valores de normalidade para indivíduos adultos. Conclui-se que estes dados, em complementação àqueles obtidos com os estudos pré-clínicos, confirmam a baixa toxicidade do produto fitoterápico.

  11. EucaTool®, a cloud computing application for estimating the growth and production of Eucalyptus globulus Labill. plantations in Galicia (NW Spain

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    Alberto Rojo-Alboreca

    2015-12-01

    Full Text Available Aim of study: To present the software utilities and explain how to use EucaTool®, a free cloud computing application developed to estimate the growth and production of seedling and clonal blue gum (Eucalyptus globulus Labill. plantations in Galicia (NW Spain.Area of study: Galicia (NW Spain.Material and methods: EucaTool® implements a dynamic growth and production model that is valid for clonal and non-clonal blue gum plantations in the region. The model integrates transition functions for dominant height (site index curves, number of stems per hectare (mortality function and basal area, as well as output functions for tree and stand volume, biomass and carbon content.Main results: EucaTool® can be freely accessed from any device with an Internet connection, from http://app.eucatool.com. In addition, useful information about the application is published on a related website: http://www.eucatool.com.Research highlights: The application has been designed to enable forest stakeholders to estimate volume, biomass and carbon content of forest plantations from individual trees, diameter classes or stand data, as well as to estimate growth and future production (indicating the optimal rotation age for maximum income by measurement of only four stand variables: age, number of trees per hectare, dominant height and basal area.Keywords: forest management; biomass; seedling; clones; blue gum; forest tool.

  12. Predicción bayesiana de parámetros genéticos en clones de Eucalyptus globulus bajo condiciones de suplemento hídrico

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    Freddy Mora

    2013-01-01

    Full Text Available Se realizó un análisis Bayesiano de parámetros genéticos del crecimiento en 29 clones de Eucalyptus globulus de doce meses de edad, en el sur de Chile. Se consideraron dos condiciones ambientales contrastantes en cuanto a la disponibilidad hídrica: 1. Sin irrigación, y 2. Las plantas se irrigaron con un sistema localizado de riego. Para el análisis Bayesiano se utilizó el algoritmo de Gibbs en un modelo de interacción clonambiente. Los valores de heredabilidad fueron altos en la condición de riego (moda a posteriori de H^2=0,41, 0,36 y 0,39 para la altura, diámetro y área basal, respectivamente, en tanto que en la situación sin riego, los valores de las heredabilidades fueron significativamente más bajos; confirmado por medio de los intervalos de credibilidad Bayesianos (95 % de probabilidad. La moda a posteriori de la correlación genética entre sitios fue positiva y alta para las tres características (r=0,7, 0,65 y 0,8, para altura, diámetro y área basal, respectivamente, y de acuerdo al intervalo de credibilidad, esta correlación fue estadísticamente diferente de cero, indicando una interacción no significativa.

  13. The effect of vapour pressure deficit on stomatal conductance, sap pH and leaf-specific hydraulic conductance in Eucalyptus globulus clones grown under two watering regimes.

    Science.gov (United States)

    Hernandez, Maria Jose; Montes, Fernando; Ruiz, Federico; Lopez, Gustavo; Pita, Pilar

    2016-05-01

    Stomatal conductance has long been considered of key interest in the study of plant adaptation to water stress. The expected increase in extreme meteorological events under a climate change scenario may compromise survival in Eucalyptus globulus plantations established in south-western Spain. We investigated to what extent changes in stomatal conductance in response to high vapour pressure deficits and water shortage are mediated by hydraulic and chemical signals in greenhouse-grown E. globulus clones. Rooted cuttings were grown in pots and submitted to two watering regimes. Stomatal conductance, shoot water potential, sap pH and hydraulic conductance were measured consecutively in each plant over 4 weeks under vapour pressure deficits ranging 0·42 to 2·25 kPa. Evapotranspiration, growth in leaf area and shoot biomass were also determined. There was a significant effect of both clone and watering regime in stomatal conductance and leaf-specific hydraulic conductance, but not in sap pH. Sap pH decreased as water potential and stomatal conductance decreased under increasing vapour pressure deficit. There was no significant relationship between stomatal conductance and leaf-specific hydraulic conductance. Stomata closure precluded shoot water potential from falling below -1·8 MPa. The percentage loss of hydraulic conductance ranged from 40 to 85 %. The highest and lowest leaf-specific hydraulic conductances were measured in clones from the same half-sib families. Water shortage reduced growth and evapotranspiration, decreases in evapotranspiration ranging from 14 to 32 % in the five clones tested. Changes in sap pH seemed to be a response to changes in atmospheric conditions rather than soil water in the species. Stomata closed after a considerable amount of hydraulic conductance was lost, although intraspecific differences in leaf-specific hydraulic conductance suggest the possibility of selection for improved productivity under water-limiting conditions

  14. Effet de l'hybridation interspecifique sur la teneur et la composition chimique des huiles essentielles d'eucalyptus cultivés au Maroc

    OpenAIRE

    Farah A.; Fechtal M.; Chaouch A.

    2002-01-01

    Interspecific hybridization effect on the content and the chemical composition of essential oils of eucalyptus grown in Morocco. In this work, the quality and quantity of the leave essential oils of five Eucalyptus hybrids were determined. The crosses of Eucalyptus globulus ssp. maideni, Eucalyptus globulus ssp. globulus, Eucalyptus grandis, Eucalyptus cladocalyx and Eucalyptus diversicolor with Eucalyptus camaldulensis have been studied. The average yield of parental species samples varies a...

  15. CARACTERIZACION DE RIZOBACTERIAS PROMOTORAS DEL ENRAIZAMIENTO DE ESTACAS DE EUCALYPTUS GLOBULUS LABILL. : MECANISMOS DE ACCION Y FLUCTUACION POBLACIONAL

    OpenAIRE

    DIAZ PERALTA, KATY PAULINA; DIAZ PERALTA, KATY PAULINA

    2009-01-01

    Las plantaciones de Eucalyptus spp en Chile, son altamente valoradas en el mercado mundial, su producción se basa en la propagación vegetativa de plantas en viveros, a través de estacas y miniestacas; sin embargo, ex iste una variabilidad en el potencial del enraizamiento entre clones de especies e híbridos del género, lo cual no permite obtener el total de plantas en los programas de producción clonal. Diversos estudios han demostrado que bacterias rizosféricas denominadas bacterias promotor...

  16. ESTUDIOS SOBRE EL COMPORTAMIENTO DE FORRAJEO DE Acromyrmex lundi Guering (HYMENOPTERA, FORMICIDAE Y SU EFECTO SOBRE EL CRECIMIENTO DE PROCEDENCIAS DE Eucalyptus globulus Labill. (Myrtaceae

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    Carmen Lidia Martínez

    2015-02-01

    Full Text Available El ataque de las hormigas cortadoras es una de las principales razones de pérdida de plantas durante la etapa de establecimiento de un monte forestal. Los objetivos de este estudio fueron evaluar la intensidad de forrajeo de Acromyrmex lundi asociado a algunas condiciones ambientales y procedencias de Eucalyptus globulus y estimar el impacto de la herbivoría sobre el crecimiento inicial de estos árboles. Dos ensayos se llevaron a cabo durante dos temporadas estivo-otoñales consecutivas, en el Campo Experimental de la Universidad Nacional de Luján, Argentina. Los materiales de diferentes procedencias fueron dispuestos en bloques equidistantes a 5 m de un nido activo de A. lundi. Durante doce semanas se contabilizó el número de hojas cortadas por las hormigas y se registraron algunas variables meteorológicas. En el segundo año la mitad de las parcelas fue protegida de las hormigas y se midieron variables de crecimiento. Los materiales de todas las procedencias fueron atacadas por A. lundi, aunque el número de hojas cosechada fue significativamente diferente sólo entre los orígenes Flinders Island (52,5 hojas y Nullo Mountain (28,5 hojas. El número de días lluviosos fue el único factor ambiental que se relacionó positivamente con la actividad de las hormigas.Todos los tratamientos redujeron significativamente la altura, el diámetro y el área foliar cuando fueron atacados por las hormigas. Las distintas procedencias compensaron diferencialemnte la herbivoría. Se discuten los resultados en función de los criterios a tener en cuenta para la selección de materiales genéticos como herramienta de manejo y control del ataque de A. lundi.

  17. Drought increases heat tolerance of leaf respiration in Eucalyptus globulus saplings grown under both ambient and elevated atmospheric [CO2] and temperature

    Science.gov (United States)

    Gauthier, Paul P. G.; Crous, Kristine Y.; Ayub, Gohar; Duan, Honglang; Weerasinghe, Lasantha K.; Ellsworth, David S.; Tjoelker, Mark G.; Evans, John R.; Tissue, David T.; Atkin, Owen K.

    2014-01-01

    Climate change is resulting in increasing atmospheric [CO2], rising growth temperature (T), and greater frequency/severity of drought, with each factor having the potential to alter the respiratory metabolism of leaves. Here, the effects of elevated atmospheric [CO2], sustained warming, and drought on leaf dark respiration (R dark), and the short-term T response of R dark were examined in Eucalyptus globulus. Comparisons were made using seedlings grown under different [CO2], T, and drought treatments. Using high resolution T–response curves of R dark measured over the 15–65 °C range, it was found that elevated [CO2], elevated growth T, and drought had little effect on rates of R dark measured at T CO2], growth T, and drought on T response of R dark. However, drought increased R dark at high leaf T typical of heatwave events (35–45 °C), and increased the measuring T at which maximal rates of R dark occurred (T max) by 8 °C (from 52 °C in well-watered plants to 60 °C in drought-treated plants). Leaf starch and soluble sugars decreased under drought and elevated growth T, respectively, but no effect was found under elevated [CO2]. Elevated [CO2] increased the Q 10 of R dark (i.e. proportional rise in R dark per 10 °C) over the 15–35 °C range, while drought increased Q 10 values between 35 °C and 45 °C. Collectively, the study highlights the dynamic nature of the T dependence of R dark in plants experiencing future climate change scenarios, particularly with respect to drought and elevated [CO2]. PMID:25205579

  18. Comparative gas chromatography-mass spectroscopy, Fourier transform infrared spectroscopy, and high-performance liquid chromatography analysis of essential oils extracted using 4 methods from the leaves of Eucalyptus globulus L.

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    Yasmeen Khan

    2016-01-01

    Full Text Available Background: Eucalyptus globulus L. (family, Myrtaceae is one of the world′s most widely planted genera. E. globulus L., commonly referred to as Tasmanian blue gum, is a fast growing, evergreen tree, native to Tasmania and South-East Australia. Apart from its extensive use in pulp industry, it is also produces Oleum Eucalypti (eucalyptus oil that is extracted on commercial scale in many countries such as China, India, South Africa, Portugal, Brazil, and Tasmania, as a raw material in perfumery, cosmetics, food beverage, aromatherapy, and phytotherapy. Materials and Methods: Traditional hydrodistillation (HD, solvent extraction (SE, ultrasonication (US, and supercritical fluid extraction (SFE were conducted for the extraction of essential oil from the leaves of E. globulus. Each oil was evaluated in terms of high-performance liquid chromatography (HPTLC and Fourier transform infrared spectroscopy (FTIR fingerprinting with qualitative and semi-quantitative composition of the isolated essential oil by gas chromatography-mass spectroscopy (GCMS, the extract yield of essential oil was 2.60%, 2.2%, 2.0%, and 3.6% v/w, respectively, for HD, SE, US, and SFE. Results: A total of 53 compounds were identified by GCMS. Comparative analysis indicated that SFE was favorable for extraction of monoterpene hydrocarbon, sesquiterpene hydrocarbon, and oxygenated sesquiterpene hydrocarbon. HD, SE, and US had certain advantages in the extraction of aliphatic saturated hydrocarbons organic acid and esters. Overlay, FTIR spectra of oil samples obtained by four extraction methods were superimposed with each other showing similar components. The maximum separation of compound seen at 254 nm and lesser at 366 nm by HPTLC fingerprinting which again showed superimposed chromatograms. Conclusion: It is concluded that different extraction method may lead to different yields of essential oils where the choice of appropriate method is very important to obtained more desired

  19. Nickel-tolerant ectomycorrhizal Pisolithus albus ultramafic ecotype isolated from nickel mines in New Caledonia strongly enhance growth of the host plant Eucalyptus globulus at toxic nickel concentrations.

    Science.gov (United States)

    Jourand, Philippe; Ducousso, Marc; Reid, Robert; Majorel, Clarisse; Richert, Clément; Riss, Jennifer; Lebrun, Michel

    2010-10-01

    Ectomycorrhizal (ECM) Pisolithus albus (Cooke & Massee), belonging to the ultramafic ecotype isolated in nickel-rich serpentine soils from New Caledonia (a tropical hotspot of biodiversity) and showing in vitro adaptive nickel tolerance, were inoculated to Eucalyptus globulus Labill used as a Myrtaceae plant-host model to study ectomycorrhizal symbiosis. Plants were then exposed to a nickel (Ni) dose-response experiment with increased Ni treatments up to 60 mg kg( - )(1) soil as extractable Ni content in serpentine soils. Results showed that plants inoculated with ultramafic ECM P. albus were able to tolerate high and toxic concentrations of Ni (up to 60 μg g( - )(1)) while uninoculated controls were not. At the highest Ni concentration tested, root growth was more than 20-fold higher and shoot growth more than 30-fold higher in ECM plants compared with control plants. The improved growth in ECM plants was associated with a 2.4-fold reduction in root Ni concentration but a massive 60-fold reduction in transfer of Ni from root to shoots. In vitro, P. albus strains could withstand high Ni concentrations but accumulated very little Ni in its tissue. The lower Ni uptake by mycorrhizal plants could not be explained by increased release of metal-complexing chelates since these were 5- to 12-fold lower in mycorrhizal plants at high Ni concentrations. It is proposed that the fungal sheath covering the plant roots acts as an effective barrier to limit transfer of Ni from soil into the root tissue. The degree of tolerance conferred by the ultramafic P. albus isolates to growth of the host tree species is considerably greater than previously reported for other ECM. The primary mechanisms underlying this improved growth were identified as reduced Ni uptake into the roots and markedly reduced transfer from root to shoot in mycorrhizal plants. The fact that these positive responses were observed at Ni concentrations commonly observed in serpentinic soils suggests that

  20. Microwave Accelerated Green Synthesis of Stable Silver Nanoparticles with Eucalyptus globulus Leaf Extract and Their Antibacterial and Antibiofilm Activity on Clinical Isolates.

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    Khursheed Ali

    Full Text Available A simple and rapid microwave assisted method of green synthesis of silver nanoparticles (AgNPs was developed using aqueous leaf extract of Eucalyptus globulus(ELE, and their antibacterial and antibiofilm potential investigated. With this aim, the aqueous solutions of ELE and AgNO3(1 mM were mixed (1:4 v/v, and microwave irradiated at 2450 Mhz, for 30 sec. The instant color change of the ELE-AgNO3 mixture from pale yellow to dark brown indicated ELE-AgNPs synthesis. The intensity of peak at 428 nm in UV-Vis spectra, due to the surface plasmon resonance of AgNPs, varied with the amount of ELE, AgNO3 concentration, pH and time of incubation. The biosynthesized ELE-AgNPs were characterized by UV-visible spectroscopy, XRD, TEM, SEM-EDX, FTIR and TGA analyses. The size of ELE-AgNPs was determined to be in range of 1.9-4.3 nm and 5-25 nm, with and without microwave treatment, respectively. SEM exhibited the capping of AgNPs with the ELE constituents, and validated by FTIR analysis. The FTIR data revealed the presence of plant organic constituents and metabolites bound to ELE-AgNPs, which contributes for their stability. The antimicrobial activity of ELE-AgNPs was assessed by growth and biofilm inhibition of extended spectrum β-lactamase (ESBL producing Pseudomonas aeruginosa, Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA and methicillin-sensitive Staphylococcus aureus (MSSA clinical bacterial isolates. The results demonstrated that S. aureus were more sensitive to ELE-AgNPs than E. coli and P. aeruginosa. MRSA exhibited higher sensitive than MSSA, whereas P. aeruginosa were more sensitive than E. coli to ELE-AgNPs treatment. Also, significant (83 ± 3% and 84 ± 5% biofilm inhibition was observed in case of S. aureus and P. aeruginosa, respectively. The results elucidated environmentally friendly, economical and quick method for production of colloidal bio-functionalized ELE-AgNPs, for effectual clinical applications, as broad

  1. Oligo-carrageenan kappa-induced reducing redox status and activation of TRR/TRX system increase the level of indole-3-acetic acid, gibberellin A3 and trans-zeatin in Eucalyptus globulus trees.

    Science.gov (United States)

    González, Alberto; Contreras, Rodrigo A; Zúiga, Gustavo; Moenne, Alejandra

    2014-08-20

    Eucalyptus globulus trees treated with oligo-carrageenan (OC) kappa showed an increase in NADPH, ascorbate and glutathione levels and activation of the thioredoxin reductase (TRR)/thioredoxin (TRX) system which enhance photosynthesis, basal metabolism and growth. In order to analyze whether the reducing redox status and the activation of thioredoxin reductase (TRR)/thioredoxin (TRX) increased the level of growth-promoting hormones, trees were treated with water (control), with OC kappa, or with inhibitors of ascorbate synthesis, lycorine, glutathione synthesis, buthionine sulfoximine (BSO), NADPH synthesis, CHS-828, and thioredoxin reductase activity, auranofine, and with OC kappa, and cultivated for four additional months. Eucalyptus trees treated with OC kappa showed an increase in the levels of the auxin indole 3-acetic acid (IAA), gibberellin A3 (GA3) and the cytokinin trans-zeatin (t-Z) as well as a decrease in the level of the brassinosteroid epi-brassinolide (EB). In addition, treatment with lycorine, BSO, CHS-828 and auranofine inhibited the increase in IAA, GA3 and t-Z as well as the decrease in EB levels. Thus, the reducing redox status and the activation of TRR/TRX system induced by OC kappa increased the levels of IAA, GA3 and t-Z levels determining, at least in part, the stimulation of growth in Eucalyptus trees.

  2. COMPORTAMENTO ESPECTRAL DE FOLHAS DE Eucalyptus globulus (Labill. ATACADAS POR Mycosphaerella spp. NAS REGIÕES DO VISÍVEL E DO INFRAVERMELHO PRÓXIMO DO ESPECTRO ELETROMAGNÉTICO

    Directory of Open Access Journals (Sweden)

    Diogo Belmonte Lippert

    2015-01-01

    Full Text Available The study aims to analyze the spectral behavior of leaves of Eucalyptus globulus attacked in four different severity levels by Mycosphaerella spp. in different positions in the canopy of the tree in the four seasons, considering two regions of the electromagnetic spectrum: visible and near infrared. The plant material was collected in all seasons, in different positions in the tree (base, middle and top of the canopy extract. In the laboratory, leaves were classified into four levels of severity (healthy, little, medium and heavy fire and then made measurements of spectral reflectance measured with a spectroradiometer. The spectral behavior of Eucalyptus globules leaves is characteristic according to the severity degree of the disease, differing primarily in the other high levels of infection by Mycosphaerella spp. and differ mainly of healthy leaves and leaves which are little attacked, in both regions of the electromagnetic spectrum, this behavior occurs for different treetop positions and climate seasons, varying the amount of reflected energy. It is concluded that the reflectance of the Eucalyptus globules leaves attacked by Mycosphaerella spp. differs according to the position in the tree crown and the weather station when they were collected and to the advanced levels of disease severity.

  3. Decomposição de agulhas de Pinus pinaster e de folhas de Eucalyptus globulus em regiões do interior e do litoral de Portugal Decomposition of needle litter of Pinus pinaster and leaf litter of Eucalyptus globulus in the littoral and inland areas of Portugal

    Directory of Open Access Journals (Sweden)

    C. Ribeiro

    2007-07-01

    Full Text Available Estudou-se, pela metodologia dos litter-bags, a decomposição de agulhas de Pinus pinaster (PP e de folhas de Eucalyptus globulus (EG, considerando a taxa de decomposição e a dinâmica de libertação dos nutrientes mais relevantes para a sustentabilidade dos sistemas florestais. Os estudos decorreram no litoral da Região Centro (Furadouro, Óbidos, quer com folhas de EG quer com agulhas de PP, no interior da Região Norte (Vila Pouca de Aguiar, com agulhas de PP, e numa situação intermédia com folhas de EG (Pegões e Rio Maior. Para igual período, a taxa de decomposição das agulhas de PP, estimada pelo modelo exponencial simples, foi inferior à determinada para as folhas de EG, sendo a diferença mais acentuada na fase inicial da decomposição (6 meses, em que a perda de peso das agulhas de PP foi cerca de metade da observada para as folhas de EG. Durante a fase inicial decomposição, tanto das folhas de EG como das agulhas de PP, ocorreu uma assinalável libertação de P, K e de Mg. A libertação do N dependeu da taxa de decomposição, observando- -se imobilização para as agulhas de PP com mais baixa taxa de decomposição, e libertação rápida para as folhas de EG com mais elevada taxa de decomposição. No caso do Ca o factor diferenciador foi a espécie, sendo a respectiva libertação baixa para as agulhas de PP, mas acentuada para as folhas de EG. As folhas verdes de resíduos de abate de EG decompuseram-se e libertaram os nutrientes mais rapidamente do que as folhas senescentes da mesma espécie.Decomposition of needle litter of Pinus pinaster (PP and leaf litter of Eucalyptus globulus (EG was assessed using the litterbag methodology. Decomposition rate of these residues and release dynamics of nutrients with more relevance to the sustainability of forest systems were measured. The study was carried out in the litoral of Central Portugal (Furadouro,Óbidos, using both PP and EG, in inland of North Portugal (Vila Pouca

  4. Kraft pulping and ECF bleaching of Eucalyptus globulus pretreated by the white-rot fungus Ceriporiopsis subvermispora - doi: 10.4025/actascitechnol.v34i3.12410

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    Claudio Salazar

    2012-05-01

    Full Text Available Eucalyptus globulus wood chips were decayed by the lignin-degrading fungus Ceriporiopsis subvermispora as a pretreatment step before kraft pulping. Weight and component losses of wood after the biotreatment were the following: weight (5%, glucans (1.5%, xylans (4.3%, lignin (5.7% and extractives (57.5%. The residual amount of lignin (expressed by the kappa number in pulps from biotreated wood chips was lower than that of pulps from the undecayed control. Depending on the delignification degree, kraft biopulps presented similar or up to 4% increase in pulp yield and 20% less hexenuronic acids (HexA than control pulps. The extended delignification with O2 decreases approximately 50% of the kappa number of the pulps and increases brightness, but had no effect in HexA reduction. The bleaching steps with chlorine dioxide (D0ED1 sequence decreased the kappa number up to 97%, increased pulp brightness up to 84% ISO and decreased HexA amount up to 91%. The use of C. subvermispora in biopulping of E. globulus generated important benefits during the production of kraft pulps that are reflected in a high pulp yield, low residual lignin content, low HexA amount, high brightness and viscosity of the biopulps as compared with pulps produced from untreated wood chips.

  5. Captura de carbono en rebrotes de eucalyptus globulus labill “eucaliptus” en motil, provincia de Otuzco del departamento de La Libertad-Perú

    OpenAIRE

    Alfaro Nureña, Rafael Otoniel

    2017-01-01

    It was determined biomass, carbon content in plots of 250 square meters in 10 stands of eucalyptus plantations in the village of Motil Agallpampa district of the province of Otuzco, La Libertad, Peru .Was determined models biomass and carbon was determined by regression analysis based on the method of least squares ,method presented by Adler (1980); for this combinations of independent variables dap and height were selected using a spreadsheet (Excel). The equations chosen were those with the...

  6. Comparative Effects of Some Medicinal Plants: Anacardium occidentale, Eucalyptus globulus, Psidium guajava, and Xylopia aethiopica Extracts in Alloxan-Induced Diabetic Male Wistar Albino Rats

    Science.gov (United States)

    Okpashi, Victor Eshu; Bayim, Bayim Peter-Robins; Obi-Abang, Margaret

    2014-01-01

    Insulin therapy and oral antidiabetic agents/drugs used in the treatment of diabetes mellitus have not sufficiently proven to control hyperlipidemia, which is commonly associated with the diabetes mellitus. Again the hopes that traditional medicine and natural plants seem to trigger researchers in this area is yet to be discovered. This research was designed to compare the biochemical effects of some medicinal plants in alloxan-induced diabetic male Wistar rats using named plants that are best at lowering blood glucose and hyperlipidemia and ameliorating other complications of diabetes mellitus by methods of combined therapy. The results obtained showed 82% decrease in blood glucose concentration after the 10th hour to the fortieth hour. There was significant increase P 0.05 recorded in the glutathione peroxidase activity of E. globulus (100 mg/kg) when compared to the test groups of P. guajava (250 mg/kg) and X. aethiopica (250 mg/kg). Catalase activity showed significant increase P 0.05, there was no significant difference seen between test group and treated groups. Meanwhile, degree of significance was observed in other parameters analysed. The biochemical analysis conducted in this study showed positive result, attesting to facts from previous works. Though these individual plants extracts exhibited significant increase in amelorating diabetes complication and blood glucose control compared to glibenclamide, a synthetic antidiabetic drug. Greater performance was observed in the synergy groups. Therefore, a poly/combined formulation of these plants extracts yielded significant result as well as resolving some other complications associated with diabetics. PMID:25525518

  7. Production of polyploids from cultured shoot tips of Eucalyptus ...

    African Journals Online (AJOL)

    Polyploids from cultured shoot tips of Eucalyptus globulus were produced by treatment with colchicine. Results showed that the combination of 0.5% colchicine and treating multiple shoot clumps for 4 days was the most appropriate conditions for E. globulus polyploidy induction and the effect of the use of multiple shoot ...

  8. 27-36, 2014 27 Quality assessment of essential oils of Eucalyptus

    African Journals Online (AJOL)

    In the course of this study, essential oils of Eucalyptus globulus and three Boswellia rivae species were analyzed using ... parts of the plant, including leaves, flowers, seeds, rhizomes ..... oil and its major component 1,8-cineole against fungal.

  9. Supplementary data: Eucalyptus microsatellites mined in silico ...

    Indian Academy of Sciences (India)

    Supplementary data: Eucalyptus microsatellites mined in silico: survey and evaluation. R. Yasodha, R. Sumathi, P. Chezhian, S. Kavitha and M. Ghosh. J. Genet. 87, XX-XX. Tm. CT. 2222. NA. 60 125. 192. Table 1. List of EST-SSR primers developed for E. globulus. No. of. Tm Product. Acc. no. SSR repeats. Forward primer.

  10. Characterization of abiotic stress genes from different species of eucalyptus

    International Nuclear Information System (INIS)

    Naz, S.; Kausar, H.; Saleem, F.; Zafarullah, A.

    2015-01-01

    The stresses causing dehydration damage to the plant cell like cold, drought, and high salinity are the most frequent environmental stresses that influence plant growth, development and restraining productivity in cultivated areas world-wide. Many drought, salinity and cold inducible genes causing tolerance to environmental stresses in many plants include Dehydrin1 (DHN1), Dehydrin2 (DHN2), Dehydrin10 (DHN10), putative phosphate transporter (Ecpt2), choline monooxygenase (CMO) and DREB/CBF1c genes. Gene specific primer pairs were designed for each gene using DNAStar software. These genes were amplified from different species of eucalyptus such as Eucalyptus camaldulensis, E. globulus, E. tereticornis and E. gunii through PCR. Dehydrin2 gene of E. camaldulensis and dehydrin10 gene of E. globulus were cloned using the TA Cloning Kit with pCR 2.1 vector and sequenced. The Dehydrin genes sequences were submitted to GeneBank: Eucalyptus globulus dehydrin10 gene (Accession No. HG915712) and E. camaldulensis dehydrin 2 gene (Accession No. HG813113). The amino acid sequence of Dehydrin10 from E. globulus showed 97% homology to E. globulus DHN10 (JN052210) and Dehydrin2 from E. camaldulensis presented 94% homology to E. globulus DHN2 (JN052209). These genes can be employed in generating drought resistant crop plants. (author)

  11. Escala diagramática para quantificação da severidade de manchas em folhas de Eucalyptus globulus Labill. causadas por Teratosphaeria nubilosa (Cooke Crous & U. Braun

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    Martha Maria Passador

    2013-01-01

    Full Text Available The leaf spot (Mycosphaerella leaf disease = MLD caused by Teratosphaeria nubilosa has caused damage in eucalypt plantations in southern and southeastern Brazil. The need to assess the disease in the field to evaluate of this damage, efficiency control, evaluation of germplasm induces to the necessity of having a visual scale for evaluation of disease. The objective was to develop a diagrammatic scale for young leaves and one for adult leaves of Eucalyptus globules for MLD. To do so, the leaves collected in the field were scanned for image analysis. The damaged area, the healthy leaf area and the external area of the same scale RGB (Red, Green, Blue were determined. Subsequently, it was determinate the levels of severity depending on the sample distribution with seven levels for young leaves and six for adult leaves. For the visual acuity test and validate the scale, the leaves were evaluated for severity, with and without scale. With this proposed scales, the assessors showed good accuracy both for young and adult leaves with R2=0,98 and R2=0,80, respectively. The importance of the development of diagrammatic scales for assessing MLD in eucalyptus must to the fact that allows quantification of the symptoms accurately and precisely.

  12. DETERMINACIÓN DE MEDIOS DE CULTIVO Y PH PARA LA MASIFICACIÓN in vitro DE CEPAS DE Suillus luteus Aubl. ASOCIADAS A Pinus radiata D. Don Y Scleroderma citrinum Pers. ASOCIADAS A Eucalyptus globulus Labill. DE LA REGIÓN DEL BIOBÍO, CHILE

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    Marta González

    2015-02-01

    Full Text Available El uso de agentes biológicos en la producción en vivero ha aflorado como un elemento que permite no sólo mejorar la calidad morfológica y fisiológica de las plantas, sino que también posibilitar un mayor éxito en el ámbito silvicultural de una plantación. Estos agentes biológicos requieren de un ambiente óptimo para crecer, asociarse y reproducirse. El objetivo de este estudio fue determinar la dependencia del pH y el medio de cultivo en el crecimiento in vitro de distintas cepas de Suillus luteus y Scleroderma citrinum asociadas a Pinus radiata y Eucalyptus globulus, respectivamente. El estudio se realizó en condiciones controladas de temperatura y humedad, disponiendo los inóculos en placas Petri con diferentes medios cultivo, evaluando parámetros de crecimiento y biomasa a los 38 días para S. luteus y 105 días para S. citrinum. Los resultados indican que tanto el medio de cultivo, el pH del medio, así como las cepas de cada especie estudiada, son determinantes en las respuestas de crecimiento de los hongos ectomicorrícicos in vitro evaluados. Las cepas de S. luteus se desarrollaron adecuadamente en un medio de cultivo con abundancia de nutrientes (BAF, MMN como en baja presencia de ellos (EMA, y con pH más bien ácido (4,8 y 5,8. Por otro lado, las cepas de S. citrinum presentaron, para los mismos ambientes, un desarrollo inferior y lento, no obstante, la cepa Sc8 se reprodujo de forma óptima y rápida bajo un medio de cultivo BAF y con un pH moderadamente ácido de 5,8.

  13. The Eucalyptus terpene synthase gene family.

    Science.gov (United States)

    Külheim, Carsten; Padovan, Amanda; Hefer, Charles; Krause, Sandra T; Köllner, Tobias G; Myburg, Alexander A; Degenhardt, Jörg; Foley, William J

    2015-06-11

    Terpenoids are abundant in the foliage of Eucalyptus, providing the characteristic smell as well as being valuable economically and influencing ecological interactions. Quantitative and qualitative inter- and intra- specific variation of terpenes is common in eucalypts. The genome sequences of Eucalyptus grandis and E. globulus were mined for terpene synthase genes (TPS) and compared to other plant species. We investigated the relative expression of TPS in seven plant tissues and functionally characterized five TPS genes from E. grandis. Compared to other sequenced plant genomes, Eucalyptus grandis has the largest number of putative functional TPS genes of any sequenced plant. We discovered 113 and 106 putative functional TPS genes in E. grandis and E. globulus, respectively. All but one TPS from E. grandis were expressed in at least one of seven plant tissues examined. Genomic clusters of up to 20 genes were identified. Many TPS are expressed in tissues other than leaves which invites a re-evaluation of the function of terpenes in Eucalyptus. Our data indicate that terpenes in Eucalyptus may play a wider role in biotic and abiotic interactions than previously thought. Tissue specific expression is common and the possibility of stress induction needs further investigation. Phylogenetic comparison of the two investigated Eucalyptus species gives insight about recent evolution of different clades within the TPS gene family. While the majority of TPS genes occur in orthologous pairs some clades show evidence of recent gene duplication, as well as loss of function.

  14. MINERAL ELEMENTS IN WOODS OF EUCALYPTUS AND BLACK WATTLE AND ITS INFLUENCE IN A BLEACHED KRAFT PULP MILL

    Directory of Open Access Journals (Sweden)

    André Fredo

    2009-09-01

    Full Text Available Effluents are one of the most important problems in a pulp mill regarding to environmental subjects. With the purpose to reduce them, the mills are closing the internal cycles and reducing the water consumption. The wood, as the most significant source of non-process elements to the system, is responsible for some troubles to the industrial process. With the aim of evaluating their intake and to offer some informations for closing the loop, the contents of Al, Ca, Cu, Fe, K, Mg, Mn, Na, Ni, and Si were analysed in Acacia mearnsii, Eucalyptus dunnii, Eucalyptus globulus globulus, Eucalyptus grandis and Eucalyptus saligna woods. Wood samples were prepared by oven burning and acidic digestion methods, following analysis by ICP (inductively coupled plasm. Using also the results from silvicultural evaluation of trees and stands, they were calculated which elements were carried out from the site in larger amounts (K, Ca, Na, Al, Mn and Si and the species which exported largest amount of these elements (Eucalyptus dunnii and Eucalyptus globulus. The species with lower growth were Eucalyptus globulus and Eucalyptus dunnii, that leads to a bigger elements input and more potential industrial troubles. It was observed a range of 3.8 (Eucalyptus grandis up to 6 (Eucalyptus dunnii kg of analised mineral elements introduced to the process per ton of umbleached pulp produced. The Acacia mearnsii showed the lower level for Fe, Mn and Ni, being useful for oxygen, ozone and peroxide bleaching. The silicon observed in woods was in low concentration although the high values of this element in industrial liquor cycle. This leads to state that there is some contamination with soil when harvesting and handling the wood. Special care must be taken with both high ash and high mineral elements species, such as Eucalyptus dunnii and Eucalyptus globulus.

  15. Utilización de Eucalyptus spp. Alternativas de plantaciones uruguayas para pulpa Kraft

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    Javier Doldán

    2011-05-01

    Full Text Available Las plantaciones de Eucalyptus globulus han mostrado desiguales tasas de crecimiento en diferentes regiones de Uruguay. Esto ha motivado la búsqueda de otros orígenes de semilla y especies para la producción de pulpa de celulosa. Propiedades papeleras de las pulpas blanqueadas (ECF de especies alternativas llevan a intuir que podrían ser atractivas para mercados de pulpa de fibra corta. En este trabajo se realiza un comparativo del E. grandis, E. dunnii, E. maidenii y E. globulus (procedencia de semilla “Jeeralang” con el E. globulus predominante en Uruguay. Se discute el potencial de estas maderas como base para una mezcla en cocción, basándose en el análisis de propiedades físicas (densidad aparente básica, propiedades pulpables (rendimiento, carga de álcali activo en cocción Kraft y consumo de madera y propiedades papeleras. En trabajos previos se han encontrado diferencias significativas entre el comportamiento pulpable del Eucalyptus globulus y Eucalyptus maidenii, sugiriendo que nosería recomendable mezclar estas especies. La misma conclusión se podría extender a las especies de Eucalyptus estudiadas. Sin embargo, teniendo en cuenta los similares requerimientos en las cargas de álcali activo, la mezcla entre especies alternativas podría ser aplicada.AbstractEucalyptus globulus plantations have shown different growth rates in different sites in Uruguay. This fact has triggered the search for other pulp wood species and seed provenance. Paper making properties of ECF bleached pulps of alternative speciessuggest that these species could be perfectly used as hardwood bleached pulp raw materials. This study intends to compare alternativeUruguayan pulpwood species E. grandis, E. dunnii, E. maidenii and “Jeeralang” a seed provenance of E. globulus to the E. globulus most widely cultivated in the country. Physical properties of wood (Basic Density, Kraft pulping performance (pulp yield, active alkali and wood consumption

  16. Eucalyptus sp. at the Intersection of Environment and Culture in Kenya

    Directory of Open Access Journals (Sweden)

    Brandy M Garrett Kluthe

    2017-04-01

    Full Text Available Members of the genus Eucalyptus are popular on small farms throughout Kenya, and include species such as Eucalyptus saligna, Eucalpytus globulus, and Eucalyptus grandis. Although they are fast-growing and perform well on marginal land, these trees are associated with negative environmental effects in Africa and elsewhere they have been introduced. In-person surveys were conducted with small farmers in Kenya to determine patterns of Eucalyptus sp. stand use and cultural importance. It was found that despite acknowledged short and long term environmental consequences, Eucalyptus sp. remains popular for medium term economic investment. These findings are consistent with other reports of Eucalyptus sp. woodlots serving as funding sources for education, health emergencies, etc., in parts of the world where bank loans are unavailable. Further, it was found that some farmers are using modified silviculture practices to ameliorate the negative effects of Eucalyptus sp., and others are seeking indigenous alternatives.           

  17. Streptomyces rhizobacteria modulate the secondary metabolism of Eucalyptus plants.

    Science.gov (United States)

    Salla, Tamiris Daros; da Silva, Ramos; Astarita, Leandro Vieira; Santarém, Eliane Romanato

    2014-12-01

    The genus Eucalyptus comprises economically important species, such as Eucalyptus grandis and Eucalyptus globulus, used especially as a raw material in many industrial sectors. Species of Eucalyptus are very susceptible to pathogens, mainly fungi, which leads to mortality of plant cuttings in rooting phase. One alternative to promote plant health and development is the potential use of microorganisms that act as agents for biological control, such as plant growth-promoting rhizobacteria (PGPR). Rhizobacteria Streptomyces spp have been considered as PGPR. This study aimed at selecting strains of Streptomyces with ability to promote plant growth and modulate secondary metabolism of E. grandis and E. globulus in vitro plants. The experiments assessed the development of plants (root number and length), changes in key enzymes in plant defense (polyphenol oxidase and peroxidase) and induction of secondary compounds(total phenolic and quercetinic flavonoid fraction). The isolate Streptomyces PM9 showed highest production of indol-3-acetic acid and the best potential for root induction. Treatment of Eucalyptus roots with Streptomyces PM9 caused alterations in enzymes activities during the period of co-cultivation (1-15 days), as well as in the levels of phenolic compounds and flavonoids. Shoots also showed alteration in the secondary metabolism, suggesting induced systemic response. The ability of Streptomyces sp. PM9 on promoting root growth, through production of IAA, and possible role on modulation of secondary metabolism of Eucalyptus plants characterizes this isolate as PGPR and indicates its potential use as a biological control in forestry.

  18. Wood density and anatomy of three Eucalyptus species: implications for hydraulic conductivity

    OpenAIRE

    Barotto, Antonio J.; Monteoliva, Silvia; Gyenge, Javier; Martínez-Meier, Alejandro; Moreno, Karen; Tesón, Natalia; Fernández, María Elena

    2017-01-01

    Aim of the study: To characterize wood anatomical traits of three Eucalyptus species that differ in wood density and ecological requirements, and to examine the relationships between some anatomical features, wood density, and theoretical xylem hydraulic conductivity (Ks).Area of study: We analyzed 86 trees from three sites of Argentina (Entre Ríos and Buenos Aires Provinces).Methods: The sampled trees were Eucalyptus globulus, E. grandis and E. viminalis ranging from 11 to 15 years old. One ...

  19. Wood density and anatomy of three Eucalyptus species: implications for hydraulic conductivity

    OpenAIRE

    Antonio J. Barotto; Silvia Monteoliva; Javier Gyenge; Alejandro Martínez-Meier; Karen Moreno; Natalia Tesón; María Elena Fernández

    2017-01-01

    Aim of the study: To characterize wood anatomical traits of three Eucalyptus species that differ in wood density and ecological requirements, and to examine the relationships between some anatomical features, wood density, and theoretical xylem hydraulic conductivity (Ks). Area of study: We analyzed 86 trees from three sites of Argentina (Entre Ríos and Buenos Aires Provinces). Methods: The sampled trees were Eucalyptus globulus, E. grandis and E. viminalis ranging from 11 to 15 years...

  20. In silico characterization of microsatellites in Eucalyptus spp.: abundance, length variation and transposon associations

    Directory of Open Access Journals (Sweden)

    Edenilson Rabello

    2005-01-01

    Full Text Available This study assessed the abundance of microsatellites, or simple sequence repeats (SSR, in 19 Eucalyptus EST libraries from FORESTs, containing cDNA sequences from five species: E. grandis, E. globulus, E. saligna, E. urophylla and E. camaldulensis. Overall, a total of 11,534 SSRs and 8,447 SSR-containing sequences (25.5% of total ESTs were identified, with an average of 1 SSR/2.5 kb when considering all motifs and 1 SSR/3.1 kb when mononucleotides were not included. Dimeric repeats were the most abundant (41.03%, followed by trimerics (36.11% and monomerics (19.59%. The most frequent motifs were A/T (87.24% for monomerics, AG/CT (94.44% for dimerics, CCG/CGG (37.87% for trimerics, AAGG/CCTT (18.75% for tetramerics, AGAGG/CCTCT (14.04% for pentamerics and ACGGCG/CGCCGT (6.30% for hexamerics. According to sequence length, Class II or potentially variable markers were the most commonly found, followed by Class III. Two sequences presented high similarity to previously published Eucalyptus sequences from the NCBI database, EMBRA_72 and EMBRA_122. Local blastn search for transposons did not reveal the presence of any transposable elements with a cut-off value of 10-50. The large number of microsatellites identified will contribute to the refinement of marker-assisted mapping and to the discovery of novel markers for virtually all genes of economic interest.

  1. Chemical Composition and in-Vitro Evaluation of the Antimicrobial and Antioxidant Activities of Essential Oils Extracted from Seven Eucalyptus Species

    Directory of Open Access Journals (Sweden)

    Abdul Ghaffar

    2015-11-01

    Full Text Available Eucalyptus is well reputed for its use as medicinal plant around the globe. The present study was planned to evaluate chemical composition, antimicrobial and antioxidant activity of the essential oils (EOs extracted from seven Eucalyptus species frequently found in South East Asia (Pakistan. EOs from Eucalyptus citriodora, Eucalyptus melanophloia, Eucalyptus crebra, Eucalyptus tereticornis, Eucalyptus globulus, Eucalyptus camaldulensis and Eucalyptus microtheca were extracted from leaves through hydrodistillation. The chemical composition of the EOs was determined through GC-MS-FID analysis. The study revealed presence of 31 compounds in E. citriodora and E. melanophloia, 27 compounds in E. crebra, 24 compounds in E. tereticornis, 10 compounds in E. globulus, 13 compounds in E. camaldulensis and 12 compounds in E. microtheca. 1,8-Cineole (56.5%, α-pinene (31.4%, citrinyl acetate (13.3%, eugenol (11.8% and terpenene-4-ol (10.2% were the highest principal components in these EOs. E. citriodora exhibited the highest antimicrobial activity against the five microbial species tested (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Aspergillus niger and Rhizopus solani. Gram positive bacteria were found more sensitive than Gram negative bacteria to all EOs. The diphenyl-1-picrylhydazyl (DPPH radical scavenging activity and percentage inhibition of linoleic acid oxidation were highest in E. citriodora (82.1% and 83.8%, respectively followed by E. camaldulensis (81.9% and 83.3%, respectively. The great variation in chemical composition of EOs from Eucalyptus, highlight its potential for medicinal and nutraceutical applications.

  2. Chemical Composition and in-Vitro Evaluation of the Antimicrobial and Antioxidant Activities of Essential Oils Extracted from Seven Eucalyptus Species.

    Science.gov (United States)

    Ghaffar, Abdul; Yameen, Muhammad; Kiran, Shumaila; Kamal, Shagufta; Jalal, Fatima; Munir, Bushra; Saleem, Sadaf; Rafiq, Naila; Ahmad, Aftab; Saba, Iram; Jabbar, Abdul

    2015-11-18

    Eucalyptus is well reputed for its use as medicinal plant around the globe. The present study was planned to evaluate chemical composition, antimicrobial and antioxidant activity of the essential oils (EOs) extracted from seven Eucalyptus species frequently found in South East Asia (Pakistan). EOs from Eucalyptus citriodora, Eucalyptus melanophloia, Eucalyptus crebra, Eucalyptus tereticornis, Eucalyptus globulus, Eucalyptus camaldulensis and Eucalyptus microtheca were extracted from leaves through hydrodistillation. The chemical composition of the EOs was determined through GC-MS-FID analysis. The study revealed presence of 31 compounds in E. citriodora and E. melanophloia, 27 compounds in E. crebra, 24 compounds in E. tereticornis, 10 compounds in E. globulus, 13 compounds in E. camaldulensis and 12 compounds in E. microtheca. 1,8-Cineole (56.5%), α-pinene (31.4%), citrinyl acetate (13.3%), eugenol (11.8%) and terpenene-4-ol (10.2%) were the highest principal components in these EOs. E. citriodora exhibited the highest antimicrobial activity against the five microbial species tested (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Aspergillus niger and Rhizopus solani). Gram positive bacteria were found more sensitive than Gram negative bacteria to all EOs. The diphenyl-1-picrylhydazyl (DPPH) radical scavenging activity and percentage inhibition of linoleic acid oxidation were highest in E. citriodora (82.1% and 83.8%, respectively) followed by E. camaldulensis (81.9% and 83.3%, respectively). The great variation in chemical composition of EOs from Eucalyptus, highlight its potential for medicinal and nutraceutical applications.

  3. Validation of reference genes from Eucalyptus spp. under different stress conditions

    Directory of Open Access Journals (Sweden)

    Moura Jullyana Cristina Magalhães Silva

    2012-11-01

    Full Text Available Abstract Background The genus Eucalyptus consists of approximately 600 species and subspecies and has a physiological plasticity that allows some species to propagate in different regions of the world. Eucalyptus is a major source of cellulose for paper manufacturing, and its cultivation is limited by weather conditions, particularly water stress and low temperatures. Gene expression studies using quantitative reverse transcription polymerase chain reaction (qPCR require reference genes, which must have stable expression to facilitate the comparison of the results from analyses using different species, tissues, and treatments. Such studies have been limited in eucalyptus. Results Eucalyptus globulus Labill, Eucalyptus urograndis (hybrid from Eucalyptus urophylla S.T. Blake X Eucalyptus grandis Hill ex-Maiden and E. uroglobulus (hybrid from E. urograndis X E. globulus were subjected to different treatments, including water deficiency and stress recovery, low temperatures, presence or absence of light, and their respective controls. Except for treatment with light, which examined the seedling hypocotyl or apical portion of the stem, the expression analyses were conducted in the apical and basal parts of the stem. To select the best pair of genes, the bioinformatics tools GeNorm and NormFinder were compared. Comprehensive analyses that did not differentiate between species, treatments, or tissue types, showed that IDH (isocitrate dehydrogenase, SAND (SAND protein, ACT (actin, and A-Tub (α-tubulin genes were the most stable. IDH was the most stable gene in all of the treatments. Conclusion Comparing these results with those of other studies on eucalyptus, we concluded that five genes are stable in different species and experimental conditions: IDH, SAND, ACT, A-Tub, and UBQ (ubiquitin. It is usually recommended a minimum of two reference genes is expression analysis; therefore, we propose that IDH and two others genes among the five identified

  4. Allelopathy in agroforestry systems: the effects of leaf extracts ofCupressus lusitanica and threeEucalyptus spp. on four Ethiopian crops

    DEFF Research Database (Denmark)

    Lisanework N.; Michelsen, Anders

    1993-01-01

    The potential allelopathic effect ofCupressus lusitanica, Eucalyptus globulus, E. camaldulensis andE. saligna on seed germination, radicle and seedling growth was investigated with four crops:Cicer arietinum (chickpea),Zea mays (maize),Pisum sativum (pea) andEragrostis tef (teff). Aqueous leaf ex...

  5. Patterns of Reproductive Isolation in Eucalyptus-A Phylogenetic Perspective.

    Science.gov (United States)

    Larcombe, Matthew J; Holland, Barbara; Steane, Dorothy A; Jones, Rebecca C; Nicolle, Dean; Vaillancourt, René E; Potts, Brad M

    2015-07-01

    We assess phylogenetic patterns of hybridization in the speciose, ecologically and economically important genus Eucalyptus, in order to better understand the evolution of reproductive isolation. Eucalyptus globulus pollen was applied to 99 eucalypt species, mainly from the large commercially important subgenus, Symphyomyrtus. In the 64 species that produce seeds, hybrid compatibility was assessed at two stages, hybrid-production (at approximately 1 month) and hybrid-survival (at 9 months), and compared with phylogenies based on 8,350 genome-wide DArT (diversity arrays technology) markers. Model fitting was used to assess the relationship between compatibility and genetic distance, and whether or not the strength of incompatibility "snowballs" with divergence. There was a decline in compatibility with increasing genetic distance between species. Hybridization was common within two closely related clades (one including E. globulus), but rare between E. globulus and species in two phylogenetically distant clades. Of three alternative models tested (linear, slowdown, and snowball), we found consistent support for a snowball model, indicating that the strength of incompatibility accelerates relative to genetic distance. Although we can only speculate about the genetic basis of this pattern, it is consistent with a Dobzhansky-Muller-model prediction that incompatibilities should snowball with divergence due to negative epistasis. Different rates of compatibility decline in the hybrid-production and hybrid-survival measures suggest that early-acting postmating barriers developed first and are stronger than later-acting barriers. We estimated that complete reproductive isolation can take up to 21-31 My in Eucalyptus. Practical implications for hybrid eucalypt breeding and genetic risk assessment in Australia are discussed. © The Author 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For

  6. Resistance to high level of Cu (Copper) by arbuscular mycorrhizal, saprobe Fungi and Eucalyptus globules

    Energy Technology Data Exchange (ETDEWEB)

    Arriagada, C.; Pereira, G.; Machuca, A.; Alvear, M.; Martin, J.; Ocampo, J.

    2009-07-01

    The effects of saprobe and arbuscular mycorrhizal (AM) fungi on growth, chorophyll, root length colonization and succinate dehydrogenase (SDH) activity was measured in Eucalyptus globulus Labill., plants growing in soil with high level of Cu were investigated. The application of Cu inhibited the development of mycelia of the saprobe fungi Fusarium concolor and Trichoderma koningii and the hyphal length of the arbuscular mycorrhizal fungi (AM) Glomus mosseaae and G. deserticola in vitro. (Author)

  7. Resistance to high level of Cu (Copper) by arbuscular mycorrhizal, saprobe Fungi and Eucalyptus globules

    International Nuclear Information System (INIS)

    Arriagada, C.; Pereira, G.; Machuca, A.; Alvear, M.; Martin, J.; Ocampo, J.

    2009-01-01

    The effects of saprobe and arbuscular mycorrhizal (AM) fungi on growth, chorophyll, root length colonization and succinate dehydrogenase (SDH) activity was measured in Eucalyptus globulus Labill., plants growing in soil with high level of Cu were investigated. The application of Cu inhibited the development of mycelia of the saprobe fungi Fusarium concolor and Trichoderma koningii and the hyphal length of the arbuscular mycorrhizal fungi (AM) Glomus mosseae and G. deserticola in vitro. (Author)

  8. Comparative transcriptional analysis provides new insights into the molecular basis of adventitious rooting recalcitrance in Eucalyptus.

    Science.gov (United States)

    de Almeida, Márcia Rodrigues; de Bastiani, Daniela; Gaeta, Marcos Letaif; de Araújo Mariath, Jorge Ernesto; de Costa, Fernanda; Retallick, Jeffrey; Nolan, Lana; Tai, Helen H; Strömvik, Martina V; Fett-Neto, Arthur Germano

    2015-10-01

    Adventitious rooting (AR) is essential in clonal propagation. Eucalyptus globulus is relevant for the cellulose industry due to its low lignin content. However, several useful clones are recalcitrant to AR, often requiring exogenous auxin, adding cost to clonal garden operations. In contrast, E. grandis is an easy-to-root species widely used in clonal forestry. Aiming at contributing to the elucidation of recalcitrance causes in E. globulus, we conducted a comparative analysis with these two species differing in rooting competence, combining gene expression and anatomical techniques. Recalcitrance in E. globulus is reversed by exposure to exogenous indole-3-acetic acid (IAA), which promotes important gene expression modifications in both species. The endogenous content of IAA was significantly higher in E. grandis than in E. globulus. The cambium zone was identified as an active area during AR, concentrating the first cell divisions. Immunolocalization assay showed auxin accumulation in cambium cells, further indicating the importance of this region for rooting. We then performed a cambium zone-specific gene expression analysis during AR using laser microdissection. The results indicated that the auxin-related genes TOPLESS and IAA12/BODENLOS and the cytokinin-related gene ARR1may act as negative regulators of AR, possibly contributing to the hard-to-root phenotype of E. globulus. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  9. The genome of Eucalyptus grandis

    Energy Technology Data Exchange (ETDEWEB)

    Myburg, Alexander A.; Grattapaglia, Dario; Tuskan, Gerald A.; Hellsten, Uffe; Hayes, Richard D.; Grimwood, Jane; Jenkins, Jerry; Lindquist, Erika; Tice, Hope; Bauer, Diane; Goodstein, David M.; Dubchak, Inna; Poliakov, Alexandre; Mizrachi, Eshchar; Kullan, Anand R. K.; Hussey, Steven G.; Pinard, Desre; van der Merwe, Karen; Singh, Pooja; van Jaarsveld, Ida; Silva-Junior, Orzenil B.; Togawa, Roberto C.; Pappas, Marilia R.; Faria, Danielle A.; Sansaloni, Carolina P.; Petroli, Cesar D.; Yang, Xiaohan; Ranjan, Priya; Tschaplinski, Timothy J.; Ye, Chu-Yu; Li, Ting; Sterck, Lieven; Vanneste, Kevin; Murat, Florent; Soler, Marçal; Clemente, Hélène San; Saidi, Naijib; Cassan-Wang, Hua; Dunand, Christophe; Hefer, Charles A.; Bornberg-Bauer, Erich; Kersting, Anna R.; Vining, Kelly; Amarasinghe, Vindhya; Ranik, Martin; Naithani, Sushma; Elser, Justin; Boyd, Alexander E.; Liston, Aaron; Spatafora, Joseph W.; Dharmwardhana, Palitha; Raja, Rajani; Sullivan, Christopher; Romanel, Elisson; Alves-Ferreira, Marcio; Külheim, Carsten; Foley, William; Carocha, Victor; Paiva, Jorge; Kudrna, David; Brommonschenkel, Sergio H.; Pasquali, Giancarlo; Byrne, Margaret; Rigault, Philippe; Tibbits, Josquin; Spokevicius, Antanas; Jones, Rebecca C.; Steane, Dorothy A.; Vaillancourt, René E.; Potts, Brad M.; Joubert, Fourie; Barry, Kerrie; Pappas, Georgios J.; Strauss, Steven H.; Jaiswal, Pankaj; Grima-Pettenati, Jacqueline; Salse, Jérôme; Van de Peer, Yves; Rokhsar, Daniel S.; Schmutz, Jeremy

    2014-06-11

    Eucalypts are the world s most widely planted hardwood trees. Their broad adaptability, rich species diversity, fast growth and superior multipurpose wood, have made them a global renewable resource of fiber and energy that mitigates human pressures on natural forests. We sequenced and assembled >94% of the 640 Mbp genome of Eucalyptus grandis into its 11 chromosomes. A set of 36,376 protein coding genes were predicted revealing that 34% occur in tandem duplications, the largest proportion found thus far in any plant genome. Eucalypts also show the highest diversity of genes for plant specialized metabolism that act as chemical defence against biotic agents and provide unique pharmaceutical oils. Resequencing of a set of inbred tree genomes revealed regions of strongly conserved heterozygosity, likely hotspots of inbreeding depression. The resequenced genome of the sister species E. globulus underscored the high inter-specific genome colinearity despite substantial genome size variation in the genus. The genome of E. grandis is the first reference for the early diverging Rosid order Myrtales and is placed here basal to the Eurosids. This resource expands knowledge on the unique biology of large woody perennials and provides a powerful tool to accelerate comparative biology, breeding and biotechnology.

  10. Evaluation of the use potential of nine species of genus Eucalyptus for production of veneers and plywood panels

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2013-06-01

    Full Text Available The objective of thisstudywas to evaluate the use potential of nine species of Eucalyptus for production of veneer sheets and multilaminated plywood panels. Veneers were cut using a pilot laminating lathe to a nominal thickness of 2.0 mm. Analysis included finding values of overall yield and yield according to three quality classes for the nine relevant species. Plywood panels were manufactured in a laboratory, consisting of five 2.0 mm veneer sheets which were bonded together with phenol-formaldehyde resin at a weight of 360 g/m2 (double line. The panels were compressed using a specific pressure of 10 kgf/cm2, a temperature of 140ºC and a pressing time of 10 minutes. Results indicated that, with the exception of E. phaeotricha and E. pellita, all other Eucalyptus species had above 50% average veneer yield after lamination. Results of glue line shear testing and static bending parallel and perpendicular demonstrated that species Eucalyptus grandis, Eucalyptus saligna, Eucalyptus dunnii, Eucalyptus globulus, Eucalyptus viminalis, Eucalyptus robusta and Eucalyptus pellita have great potential within the parameters of this study for use in the production of veneer sheets and plywood panels intended for outdoor use.

  11. Eucalyptus oil poisoning.

    OpenAIRE

    Patel, S; Wiggins, J

    1980-01-01

    Accidental ingestion of eucalyptus oil by a 3-year-old boy caused profound central nervous system depression within 30 minutes, but he recovered rapidly after gastric lavage. The extreme toxicity of eucalyptus oil is emphasised.

  12. Transfer Rates of 238U and 232Th for E. globulus, A. mearnsii, H. filipendula and Hazardous Effects of the Usage of Medicinal Plants From Around Gold Mine Dump Environs

    Directory of Open Access Journals (Sweden)

    Victor M. Tshivhase

    2015-12-01

    Full Text Available Medicinal plant consumption can be a source of human exposure to radioactive elements such as 238U and 232Th, which can lead to internal radiation doses. The uptake of 238U and 232Th from soils to the leaf samples of three different medicinal plant species (Eucalyptus globulus, Acacia mearnsii and Hyparrhenia filipendula from the purlieu of the Princess gold mine dump, an abandoned contaminated tailings storage site (TSS, located at longitude 27°55′00″E and latitude 26°09′30″S in Davidsonville (Roodepoort, west of Johannesburg, South Africa was measured. This was done using ICP-MS spectrometry and substantial differences were observed in the soil-plant transfer factor (TF values between these radionuclides. The plant species E. globulus exhibited the highest uptake of 238U, with an average TF of 3.97, while that of H. filipendula was 0.01 and the lowest TF of 0.15 × 10−2 was measured for A. mearnsii. However, in the case of 232Th, the highest average TF was observed for A. mearnsii (0.29, followed by E. globulus (0.10 and lowest was measured for H. filipendula (0.27 × 10−2. The ratio of TF average value i.e., 238U to 232Th in the soil-plant leaves was 38.05 for E. globulus, 0.01 for A. mearnsii and 4.38 for H. filipendula.

  13. Transfer Rates of 238U and 232Th for E. globulus, A. mearnsii, H. filipendula and Hazardous Effects of the Usage of Medicinal Plants From Around Gold Mine Dump Environs

    Science.gov (United States)

    Tshivhase, Victor M.; Njinga, Raymond L.; Mathuthu, Manny; Dlamini, Thulani C.

    2015-01-01

    Medicinal plant consumption can be a source of human exposure to radioactive elements such as 238U and 232Th, which can lead to internal radiation doses. The uptake of 238U and 232Th from soils to the leaf samples of three different medicinal plant species (Eucalyptus globulus, Acacia mearnsii and Hyparrhenia filipendula) from the purlieu of the Princess gold mine dump, an abandoned contaminated tailings storage site (TSS), located at longitude 27°55′00″E and latitude 26°09′30″S in Davidsonville (Roodepoort, west of Johannesburg, South Africa) was measured. This was done using ICP-MS spectrometry and substantial differences were observed in the soil-plant transfer factor (TF) values between these radionuclides. The plant species E. globulus exhibited the highest uptake of 238U, with an average TF of 3.97, while that of H. filipendula was 0.01 and the lowest TF of 0.15 × 10−2 was measured for A. mearnsii. However, in the case of 232Th, the highest average TF was observed for A. mearnsii (0.29), followed by E. globulus (0.10) and lowest was measured for H. filipendula (0.27 × 10−2). The ratio of TF average value i.e., 238U to 232Th in the soil-plant leaves was 38.05 for E. globulus, 0.01 for A. mearnsii and 4.38 for H. filipendula. PMID:26690462

  14. Exotic Eucalyptus leaves are preferred over tougher native species but affect the growth and survival of shredders in an Atlantic Forest stream (Brazil.

    Directory of Open Access Journals (Sweden)

    Walace P Kiffer

    Full Text Available We evaluated the effect of leaves of native and exotic tree species on the feeding activity and performance of the larvae of Triplectides gracilis, a typical caddisfly shredder in Atlantic Forest streams. Leaves of four native species that differ in chemistry and toughness (Hoffmannia dusenii, Miconia chartacea, Myrcia lineata and Styrax pohlii and the exotic Eucalyptus globulus were used to determine food preferences and rates of consumption, production of fine particulate organic matter (FPOM, growth and survival of shredders. We hypothesized that the consumption rates of leaves of Eucalyptus and their effects on the growth and survival of shredders could be predicted by leaf chemistry and toughness. The larvae preferred to feed on soft leaves (H. dusenii and M. chartacea independently of the content of nutrients (N and P and secondary compounds (total phenolics. When such leaves were absent, they preferred E. globulus and did not consume the tough leaves (M. lineata and S. pohlii. In monodietary experiments, leaf consumption and FPOM production differed among the studied leaves, and the values observed for the E. globulus treatments were intermediate between the soft and tough leaves. The larvae that fed on H. dusenii and M. chartacea grew constantly over five weeks, while those that fed on E. globulus lost biomass. Larval survival was higher on leaves of H. dusenii, M. chartacea and S. pohlii than on E. globulus and M. lineata leaves. Although E. globulus was preferred over tougher leaves, long-term consumption of leaves of the exotic species may affect the abundance of T. gracilis in the studied stream. Additionally, our results suggest that leaf toughness can be a determining factor for the behavior of shredders where low-quality leaves are abundant, as in several tropical streams.

  15. Exotic Eucalyptus leaves are preferred over tougher native species but affect the growth and survival of shredders in an Atlantic Forest stream (Brazil).

    Science.gov (United States)

    Kiffer, Walace P; Mendes, Flavio; Casotti, Cinthia G; Costa, Larissa C; Moretti, Marcelo S

    2018-01-01

    We evaluated the effect of leaves of native and exotic tree species on the feeding activity and performance of the larvae of Triplectides gracilis, a typical caddisfly shredder in Atlantic Forest streams. Leaves of four native species that differ in chemistry and toughness (Hoffmannia dusenii, Miconia chartacea, Myrcia lineata and Styrax pohlii) and the exotic Eucalyptus globulus were used to determine food preferences and rates of consumption, production of fine particulate organic matter (FPOM), growth and survival of shredders. We hypothesized that the consumption rates of leaves of Eucalyptus and their effects on the growth and survival of shredders could be predicted by leaf chemistry and toughness. The larvae preferred to feed on soft leaves (H. dusenii and M. chartacea) independently of the content of nutrients (N and P) and secondary compounds (total phenolics). When such leaves were absent, they preferred E. globulus and did not consume the tough leaves (M. lineata and S. pohlii). In monodietary experiments, leaf consumption and FPOM production differed among the studied leaves, and the values observed for the E. globulus treatments were intermediate between the soft and tough leaves. The larvae that fed on H. dusenii and M. chartacea grew constantly over five weeks, while those that fed on E. globulus lost biomass. Larval survival was higher on leaves of H. dusenii, M. chartacea and S. pohlii than on E. globulus and M. lineata leaves. Although E. globulus was preferred over tougher leaves, long-term consumption of leaves of the exotic species may affect the abundance of T. gracilis in the studied stream. Additionally, our results suggest that leaf toughness can be a determining factor for the behavior of shredders where low-quality leaves are abundant, as in several tropical streams.

  16. Furfural production from Eucalyptus wood using an Acidic Ionic Liquid.

    Science.gov (United States)

    Peleteiro, Susana; Santos, Valentín; Garrote, Gil; Parajó, Juan Carlos

    2016-08-01

    Eucalyptus globulus wood samples were treated with hot, compressed water to separate hemicelluloses (as soluble saccharides) from a solid phase mainly made up of cellulose and lignin. The liquid phase was dehydrated, and the resulting solids (containing pentoses as well as poly- and oligo- saccharides made up of pentoses) were dissolved and reacted in media containing an Acidic Ionic Liquid (1-butyl-3-methylimidazolium hydrogen sulfate) and a co-solvent (dioxane). The effects of the reaction time on the product distribution were studied at temperatures in the range 120-170°C for reaction times up to 8h, and operational conditions leading to 59.1% conversion of the potential substrates (including pentoses and pentose structural units in oligo- and poly- saccharides) into furfural were identified. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. Temporal optimisation of fuel treatment design in blue gum (Eucalyptus globulus) plantations

    Science.gov (United States)

    Ana Martin; Brigite Botequim; Tiago M. Oliveira; Alan Ager; Francesco Pirotti

    2016-01-01

    This study was conducted to support fire and forest management planning in eucalypt plantations based on economic, ecological and fire prevention criteria, with a focus on strategic prioritisation of fuel treatments over time. The central objective was to strategically locate fuel treatments to minimise losses from wildfire while meeting budget constraints and demands...

  18. Resource Communication. Temporal optimization of fuel treatment design in blue gum (Eucalyptus globulus plantations

    Directory of Open Access Journals (Sweden)

    Ana Martin

    2016-07-01

    Material and methods: At each of four temporal stages (2015-2018-2021-2024 we simulated: (1 surface and canopy fuels, timber volume (m3 ha-1 and carbon storage (Mg ha-1; (2 fire behaviour characteristics, i.e. rate of spread (m min-1, and flame length (m, with FlamMap fire modelling software; (3 optimal treatment locations as determined by the Landscape Treatment Designer (LTD. Main results: The higher pressure of fire behaviour in the earlier stages of the study period triggered most of the spatial fuel treatments within eucalypt plantations in a juvenile stage. At later stages fuel treatments also included shrublands areas. The results were consistent with observations and simulation results that show high fire hazard in juvenile eucalypt stands. Research highlights: Forest management planning in commercial eucalypt plantations can potentially accomplish multiple objectives such as augmenting profits and sustaining ecological assets while reducing wildfire risk at landscape scale. However, limitations of simulation models including FlamMap and LTD are important to recognise in studies of long term wildfire management strategies. Keywords: Eucalypt plantations; Fire hazard; FlamMap; fuel treatment optimisation; Landscape Treatment Designer; wildfire risk management.

  19. Antimicrobial potential of Eucalyptus globulus against biofilms of Staphylococcus aureus isolated from bovine mastitis

    OpenAIRE

    Gomes, F. I.; Martins, Natália; Ferreira, Isabel C. F. R.; Henriques, Mariana

    2016-01-01

    Staphylococcus aureus are among the most common species isolated from bovine mastitis. The pathogenesis of this bacterium is facilitated by a number of virulence factors, including the ability to adhere to abiotic surfaces and/or host tissues often leading to biofilms' formation. From the clinical perspective, the most important feature of Staphytococcus species' biofilms is their high tolerance to the conventional antimicrobial therapy. So, the increasing number of bovine m...

  20. Acaricidal and repellent activities of essential oil of Eucalyptus globulus against Dermanyssus gallinae (Acari: Mesostigmata)

    OpenAIRE

    Dehghani-Samani Amir; Madreseh-Ghahfarokhi Samin; Dehghani-Samani Azam; Pirali-Kheirabadi Khodadad

    2015-01-01

    Introduction: By considering an increase in drug resistance against red mites, finding the nonchemical herbal acaricide against Dermanyssus gallinae (De Geer) (Acari: Mesostigmata) is necessary to kill them and to reduce the chemical resistance against chemical acaricides in this specie. Dermanyssus gallinae is a potential vector of the causal agent of several viral diseases such as Equine encephalitis and St. Louis encephalitis. It can be a vector of bacteria such as Salmonella spp., Mycobac...

  1. Genome-wide variation in recombination rate in Eucalyptus.

    Science.gov (United States)

    Gion, Jean-Marc; Hudson, Corey J; Lesur, Isabelle; Vaillancourt, René E; Potts, Brad M; Freeman, Jules S

    2016-08-09

    Meiotic recombination is a fundamental evolutionary process. It not only generates diversity, but influences the efficacy of natural selection and genome evolution. There can be significant heterogeneity in recombination rates within and between species, however this variation is not well understood outside of a few model taxa, particularly in forest trees. Eucalypts are forest trees of global economic importance, and dominate many Australian ecosystems. We studied recombination rate in Eucalyptus globulus using genetic linkage maps constructed in 10 unrelated individuals, and markers anchored to the Eucalyptus reference genome. This experimental design provided the replication to study whether recombination rate varied between individuals and chromosomes, and allowed us to study the genomic attributes and population genetic parameters correlated with this variation. Recombination rate varied significantly between individuals (range = 2.71 to 3.51 centimorgans/megabase [cM/Mb]), but was not significantly influenced by sex or cross type (F1 vs. F2). Significant differences in recombination rate between chromosomes were also evident (range = 1.98 to 3.81 cM/Mb), beyond those which were due to variation in chromosome size. Variation in chromosomal recombination rate was significantly correlated with gene density (r = 0.94), GC content (r = 0.90), and the number of tandem duplicated genes (r = -0.72) per chromosome. Notably, chromosome level recombination rate was also negatively correlated with the average genetic diversity across six species from an independent set of samples (r = -0.75). The correlations with genomic attributes are consistent with findings in other taxa, however, the direction of the correlation between diversity and recombination rate is opposite to that commonly observed. We argue this is likely to reflect the interaction of selection and specific genome architecture of Eucalyptus. Interestingly, the differences amongst

  2. Bioenergy for District Bioheating System (DBS) from eucalyptus residues in a European coal-producing region

    International Nuclear Information System (INIS)

    Paredes-Sánchez, José P.; López-Ochoa, Luis M.; López-González, Luis M.; Xiberta-Bernat, Jorge

    2016-01-01

    Highlights: • The paper introduces a combined method to evaluate bioenergy. • Forest biomass needs to be studied as a fuel supplier and carbon sink. • The forests under study produce about 28 kt dry and 0.15 Mt CO 2 per year. • Examined a District Bioheating System (DBS) with the available biomass. - Abstract: Since forest biomass can substitute for CO 2 -emitting fossil fuels in the energy sector, forest management can greatly affect the global carbon cycle. Eucalyptus globulus has adapted very well in the coal region of the Principality of Asturias (Northwestern Spain) and has become highly regarded as a valuable raw material for the pulp and paper industry. In the present work, the Eucalyptus globulus is studied as a key natural energy source in order to improve existing methods and develop new ways of optimizing the evaluation and use of both forest biomass and woody residue in energy systems, in accordance with sustainable forestry industry safety and environmental requirements. The feasibility of utilizing forest biomass instead of natural gas in a District Bioheating System (DBS) has been examined based on an analysis of its economical and environmental impacts.

  3. Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species

    Directory of Open Access Journals (Sweden)

    Cintia V. Acuña

    2014-12-01

    Full Text Available Aim of study:  To analyze the feasibility of extrapolating conclusions on wood quality genetic control between different Eucalyptus species, particularly from species with better genomic information, to those less characterized. For this purpose, the first step is to analyze the conservation and cross-transferability of microsatellites markers (SSRs located in candidate genes.Area of study: Eucalyptus species implanted in Argentina coming from different Australian origins.Materials and methods: Twelve validated and polymorphic SSRs in candidate genes (SSR-CGs for wood quality in E. globulus were selected for cross species amplification in six species: E. grandis, E. saligna, E. dunnii, E. viminalis, E. camaldulensis and E. tereticornis.Main results: High cross-species transferability (92% to 100% was found for the 12 polymorphic SSRs detected in E. globulus. These markers revealed allelic diversity in nine important candidate genes: cinnamoyl CoA reductase (CCR, cellulose synthase 3 (CesA3, the transcription factor LIM1, homocysteine S-methyltransferase (HMT, shikimate kinase (SK, xyloglucan endotransglycosylase 2 (XTH2, glutathione S-transferase (GST, glutamate decarboxylase (GAD and peroxidase (PER.Research highlights: The markers described are potentially suitable for comparative QTL mapping, molecular marker assisted breeding (MAB and for population genetic studies across different species within the subgenus Symphyomyrtus.Keywords: validation; cross-transferability; SSR; functional markers; eucalypts; Symphyomyrtus.

  4. Dry mass allocation, water use efficiency and delta C-13 in clones of Eucalyptus grandis, E-grandis x camaldulensis and E-grandis x nitens grown under two irrigation regimes

    CSIR Research Space (South Africa)

    Le Roux, D

    1996-05-01

    Full Text Available - cial clones of Eucalyptus grandis W. Hill ex Maiden. implying that less water-use-efficient trees were more productive (Bond and Stock 1990). Similarly, growing season WUE and delta13C were positively correlated in western larch and Eucalyptus globulus... regimes DEBBIE LE ROUX,1,2 WILLIAM D. STOCK,3 WILLIAM J. BOND3 and DAVID MAPHANGA4 1 Division of Forest Science and Technology, CSIR, Pretoria, South Africa 2 Present address: Department of Botany, University of Kansas, Lawrence, KS 66045, USA 3 Department...

  5. Chemical composition and fumigant toxicity of the essential oils from 16 species of Eucalyptus against Haematobia irritans (Diptera: Muscidae) adults

    OpenAIRE

    Juan, Laura W.; Lucía Mulas, Alejandro; Zerba, Eduardo N.; Harrand, Leonel; Marco, Martín; Masuh, Hector M.

    2011-01-01

    Oils extracted from various species of Eucalyptus (Eucalyptus badjensis Beuzev & Welch, Eucalyptus badjensis x Eucalyptus nitens, Eucalyptus benthamii variety dorrigoensis Maiden & Cambage, Eucalyptus botryoides Smith, Eucalyptus dalrympleana Maiden, Eucalyptus fastigata Deane & Maiden, Eucalyptus nobilis L.A.S. Johnson & K. D. Hill, Eucalyptus polybractea R. Baker, Eucalyptus radiata ssp. radiata Sieber ex Spreng, Eucalyptus resinifera Smith, Eucalyptus robertsonii Blakely, Eucalyptus rubida...

  6. Genomic research in Eucalyptus.

    Science.gov (United States)

    Poke, Fiona S; Vaillancourt, René E; Potts, Brad M; Reid, James B

    2005-09-01

    Eucalyptus L'Hérit. is a genus comprised of more than 700 species that is of vital importance ecologically to Australia and to the forestry industry world-wide, being grown in plantations for the production of solid wood products as well as pulp for paper. With the sequencing of the genomes of Arabidopsis thaliana and Oryza sativa and the recent completion of the first tree genome sequence, Populus trichocarpa, attention has turned to the current status of genomic research in Eucalyptus. For several eucalypt species, large segregating families have been established, high-resolution genetic maps constructed and large EST databases generated. Collaborative efforts have been initiated for the integration of diverse genomic projects and will provide the framework for future research including exploiting the sequence of the entire eucalypt genome which is currently being sequenced. This review summarises the current position of genomic research in Eucalyptus and discusses the direction of future research.

  7. Uji Infeksi Mycosphaerella spp Terhadap Bibit Eucalyptus spp

    OpenAIRE

    Lidya Morita Sondang

    2009-01-01

    Tujuan penelitian ini adalah untuk mengetahui tingkat ketahanan 2 klon Eucalyptus spp yaitu Eucalyptus grandis x Eucalyptus pellita dan Eucalyptus grandis x Eucalyptus urophylla terhadap Mycosphaerella spp serta mengetahui virulensi Mycospaherella spp pada 2 kelas umur (2 dan 3 bulan) pada tanaman Eucalyptus spp. Penelitian ini dilaksanakan dengan pengambilan sampel bibit tanaman Eucalyptus grandis x Eucalyptus pellita dan Eucalyptus grandis x Eucalyptus urophylla dari pembibitan PT.Toba Pulp...

  8. on black ironbark (Eucalyptus

    African Journals Online (AJOL)

    (Apis meOifera) on black ironbark. (Eucalyptus sideroxylon). B. Buys. Plant Protection Research Institute, Private Bag X5017,. Stellenbosch, 7600 Republic of South Africa. Received May /984; accepted 28 November /986. Black ironbark trees secrete nectar during the night. Argentine ants collected 42% of the nectar before ...

  9. In silico analysis of Eucalyptus thioredoxins

    Directory of Open Access Journals (Sweden)

    Aulus Estevão Barbosa

    2005-01-01

    Full Text Available The Eucalyptus Genome Sequencing Project (FORESTs, an initiative from the Brazilian ONSA consortium (Organization for Nucleotide Sequencing and Analysis, has achieved the sequencing of 123.889 EST clones from 18 different cDNA libraries. We have investigated the FORESTs data set to identify EST clusters potentially encoding thioredoxins (TRX. Two types of thioredoxin families described in plants, chloroplastic (TRXm/f/x/y and cytosolic (TRXh, have been found in the transcriptome. Putative typical TRXs have been identified in fifteen clusters, four m-type, seven h-type, two f-type, one cluster for each x/y-types and one putative homologue of the TDX gene from Arabidopsis thaliana. One cluster presents an atypical active site WCMPS, different from the conserved WCGPC present in the other 15 clusters, and corresponds to a subgroup of cytosolic thioredoxins. Except in specific libraries from callus, roots, seedlings and wood tissues, thioredoxin deduced ESTs are found in all remaining libraries. According to the calculated frequencies of ESTs, chloroplastic thioredoxins are preferentially present in green tissues such as leaves whilst cytoplasmic thioredoxins are more general but demonstrate elevated frequencies in seedlings and flower tissues. TRX frequency patterns in the Eucalyptus transcriptome seem to indicate a good coherence with data from Arabidopsis thaliana gene expression.

  10. Effect of age on calorific value and some mechanical properties of three Eucalyptus species grown in Ethiopia

    Energy Technology Data Exchange (ETDEWEB)

    Mulugeta Lemenih; Tsegaye Bekele [Wondo Genet College of Forestry, Shashemene (Ethiopia)

    2004-09-01

    The effect of age on calorific value, specific gravity, modulus of rupture (MOR) and modulus of elasticity (MOE) of three plantation grown Eucalyptus species in the south-central highlands of Ethiopia was investigated. The species investigated were Eucalyptus globulus Labill., E. saligna Sm. and E grandis W. Hill ex Maid. The investigation was made on sample trees taken from age sequences of stands of the three species. Calorific values were determined on sample discs taken at 4, 8, 12 and 16 m up the stems of the sample trees. Calorific values were also determined for heartwood and sapwood of each disc separately. Specific gravity, MOR and MOE were investigated on a 1 -m long sample log taken from the butt end of each sample tree. The results showed that there was no significant difference in calorific values between the three species when considering a similar age category. Similarly, there was no significant difference in calorific values between disc positions and wood types (heartwood versus sapwood) within species. However, calorific values tend to increase along the tree stem towards the treetop for each of the three species. Correlation between calorific values of each disc position and tree age was generally weak and dominantly negative for E. saligna and E. globulus, but positive for E. grandis. On the other hand, there was a weak but positive correlation between tree age and specific gravity and MOR for the three species. MOE was almost non-correlated with age. Generally, the results showed that waiting for older ages for the three Eucalyptus species may not further increase their calorific values. Hence, stands of the three Eucalyptus species can be harvested and used for fuelwood at an early age as traditionally managed on short-rotation basis. (author)

  11. Permeability measuremens of brazilian Eucalyptus

    Directory of Open Access Journals (Sweden)

    Marcio Rogério da Silva

    2010-09-01

    Full Text Available The permeability of Brazilian Eucalyptus grandis and Eucalyptus citriodora wood was measured in a custom build gas analysis chamber in order to determine which species could be successfully treated with preservatives. Liquid permeability was tested using an emulsion of Neen oil and a control of distillated water. Air was used to test the gas phase permeability. For both Eucalyptus grandis and Eucalyptus citriodora, the longitudinal permeability of gas was shown to be about twice as great as the liquid phase permeability. No radial permeability was observed for either wood. The permeability of air and water through the sapwood of Eucalyptus grandis was greater than that through the sapwood of Eucalyptus citriodora. The permeability of neen oil preservative through the sapwood of Eucalyptus grandis was also greater than through the sapwood of E. Citradora, but the difference was not statistically significant. Scanning Electron Microscopy images showed that the distribution and obstruction in the vessels could be correlated with observed permeability properties. Irrespective of the causes of differences in permeability between the species, the fluid phase flux through the sapwood of both species was significant, indicating that both Eucalyptus grandis and Eucalyptus citriodora could be successfully treated with wood preservative.

  12. Establishing fuelwood plantation and fire wood tree crop performance on the highlands of Ethiopia: The case of Eucalyptus globulus Labill.ssp globulus

    Energy Technology Data Exchange (ETDEWEB)

    Mehari, A.

    1997-11-01

    This study reviews reasons for the establishment of fuelwood plantation and use of fuelwood in Ethiopia. The present and future status of fire wood and the environmental degradation and related consequences are also reviewed. 138 refs, 22 figs, 6 tabs

  13. Seletivicty of eucalyptus genotypes to herbicides rates / Seletividade de genótipos de eucalipto a doses de herbicidas

    Directory of Open Access Journals (Sweden)

    Siumar Pedro Tironi

    2010-09-01

    Full Text Available The aim of this work was to quantify the herbicide selectivity applied at post-emergence of eucalyptus in different rates. The experiment was conducted in greenhouse conditions in the period from January to March 2007 at the Faculdade de Agronomia Eliseu Maciel in Universidade Federal de Pelotas – Capão do Leão-RS. Experimental design was completely randomized, with four replications. Treatments were in a factorial scheme: factor A - eucalyptus genotypes (E. globulus e E. saligna, factor B - herbicides (isoxaflutole, oxyfluorfen, bispyribac-sodium and sethoxydim+diclosulam, and factor C – rates of the registered average for weed control (0, 50, 100, 150 e 200%. Phytotoxicity, height, stem diameter and dry matter were evaluated. E. globulus genotype was more sensitive to herbicides than E. saligna. The mixture of the herbicides sethoxydim plus diclosulam showed potential to be used in eucalyptus. Bispyribac-sodium herbicide was the most toxic and isoxaflutole was the most selective to the studied genotypes.O objetivo deste trabalho foi quantificar a seletividade de herbicidas aplicados em pós-emergência na cultura do eucalipto em função de diferentes doses. O experimento foi realizado em casa de vegetação, pertencente a Faculdade de Agronomia Eliseu Maciel da Universidade Federal de Pelotas, no Município de Capão do Leão – RS, no período de janeiro a março de 2007. O delineamento experimental utilizado foi completamente casualizado, com quatro repetições. Os tratamentos foram arranjados em esquema fatorial, onde o fator A testou genótipos de eucalipto (E. globulus e E. saligna, o fator B comparou quatro herbicidas (isoxaflutole, oxyfluorfen, bispyribac-sodium e sethoxydim+diclosulam e o fator C doses (0, 50, 100, 150 e 200% da dose média registrada para controle de plantas daninhas. As variáveis avaliadas foram fitotoxicidade, estatura, diâmetro do caule e matéria seca da parte aérea das plantas de eucalipto. O gen

  14. Water relations of Eucalyptus nitens x Eucalyptus grandis : is there ...

    African Journals Online (AJOL)

    Water relations of Eucalyptus nitens x Eucalyptus grandis : is there interclonal variation in response to experimentally imposed water stress? ... Southern Forests: a Journal of Forest Science ... However, water stress reduced shoot hydraulic conductance and stem hydraulic conductivity with significant interclonal effects.

  15. Amenability of Acacia and Eucalyptus Hardwood Pulps to Elemental Chlorine-Free Bleaching: Application and Efficacy of Microbial Xylanase

    Directory of Open Access Journals (Sweden)

    Avdhesh Kumar Gangwar

    2015-10-01

    Full Text Available This study outlines the results of a biobleaching study of acacia (A. mangium and eucalyptus (E. globulus hardwood kraft pulps with commercial xylanase (Optimase CX 72 L. The comparative study was carried out using an elemental chlorine-free (ECF bleaching sequence (D0EPD1D2 after the enzyme (X stage. The enzyme treatment resulted in improved optical properties with a reduction in bleach chemical consumption. At an equivalent bleach chemical consumption, a brightness gain of 2.1 and 1.7 units and a whiteness gain of 2.7 and 2.3 units were observed with xylanase treatment in acacia and eucalyptus pulps, respectively. In ECF bleaching using the D0EPD1D2 sequence, a final brightness was achieved to the extent of 90% ISO and 89% ISO for acacia and eucalyptus, respectively, at an equivalent charge of bleach chemicals. The post-color (PC number was also reduced by up to 45% for both hardwood pulps compared with the control. The bleachability of acacia was observed to be significantly higher than that of eucalyptus. In addition, a 17.0% and 23.0% reduction in chlorine dioxide and sodium hydroxide, respectively, were obtained for both hardwood pulps after xylanase pre-bleaching, thus indicating an environmentally friendly approach to the process.

  16. Eucalyptus microsatellites mined in silico

    Indian Academy of Sciences (India)

    https://www.ias.ac.in/article/fulltext/jgen/087/01/0021-0025 ... Eucalyptus is an important short rotation pulpy woody plant, grown widely in the tropics. ... In this study, in silico analysis of 15,285 sequences representing partial and full-length mRNA from Eucalyptus species for their use in developing SSRs or microsatellites ...

  17. Cambios anatómicos y morfológicos en plántulas de Eucalyptus sp. en respuesta a diferentes niveles de luz Anatomical and morphological changes in Eucalyptus sp. seedlings in response to different levels of light

    Directory of Open Access Journals (Sweden)

    Ana B. Guarnaschelli

    2009-12-01

    Full Text Available Se evaluaron respuestas en la anatomía y morfología de plántulas de Eucalyptus globulus subesp. globulus, Eucalyptus grandis y Eucalyptus camaldulensis creciendo bajo diferentes niveles de luz. Las plantas fueron expuestas a tres regímenes de iluminación: pleno sol (C, 50 % (S50 y 75% de restricción lumínica (S75 y regadas diariamente. Se midieron características morfológicas y anatómicas del follaje, se calcularon coeficientes foliares e índices de plasticidad. Al disminuir la disponibilidad lumínica, las plantas mostraron cambios asociados a la aclimatación a la sombra. Se observaron aumentos en el área foliar y en los coeficientes foliares. Paralelamente se detectaron disminuciones en el espesor de las hojas, de la epidermis, del parénquima en empalizada y en la densidad de estomas. La biomasa foliar y la biomasa total diminuyeron significativamente bajo el nivel S75. El aumento en el nivel de restricción lumínica produjo cambios proporcionales en el espesor de hojas y en el coeficiente de área foliar. Independientemente del nivel de sombra, se detectaron diferencias entre especies, que podrían asociarse a las condiciones ambientales de sus áreas de origen. Si bien se observaron algunas diferencias en los índices de plasticidad, los valores promedio de las tres especies fueron similares. Esto sugiere que, para los caracteres considerados en este estudio, el grado de aclimatación a la sombra fue similar.Responses in the anatomy and morphology of Eucalyptus globulus subsp. globulus, Eucalyptus grandis and Eucalyptus camaldulensis seedlings, growing under different levels of light were evaluated. Seedlings were submitted to three levels of irradiance: full sun (C, 50% (S50 and 75% of light restriction (S75 and irrigated daily. Morphological and anatomical characteristics were measured; foliar coefficients and indexes of plasticity were calculated. As light availability diminished, plants showed changes associated with shade

  18. Wood density and anatomy of three Eucalyptus species: implications for hydraulic conductivity

    International Nuclear Information System (INIS)

    Barotto, A.J.; Monteoliva, S.; Gyenge, J.; Martínez-Meier, A.; Moreno, K.; Tesón, N.; Fernández, M.E.

    2017-01-01

    Aim of the study: To characterize wood anatomical traits of three Eucalyptus species that differ in wood density and ecological requirements, and to examine the relationships between some anatomical features, wood density, and theoretical xylem hydraulic conductivity (Ks). Area of study: We analyzed 86 trees from three sites of Argentina (Entre Ríos and Buenos Aires Provinces). Methods: The sampled trees were Eucalyptus globulus, E. grandis and E. viminalis ranging from 11 to 15 years old. One stem disc was cut from each tree to determine wood density and identify quantitative anatomical features of vessels and fibers. Vessel composition (S, size - to-number ratio, a measure of vessel size distribution) and lumen fraction (F, the total sapwood area available for water transport) were estimated. Results: E. grandis, the species with the highest growth rates, presented the highest theoretical Ks. This was associated with anatomical features such as a high density of wide vessels resulting in high F. On the other hand, E. viminalis, the species with the lowest growth rates and highest resistance to environmental stress, showed lower Ks as a result of a low density of wide vessels. These two species differed not only greatly in wood density but also in fiber characteristics. In the case of E. globulus, vessels were relatively narrow, which resulted in the lowest theoretical Ks, fibers were small, and wood density intermediate. Research highlights: F had greater influence on Ks than S. The anatomical characteristics and wood density could only partly explain the differential growth or resistance to stress of the studied species.

  19. Wood density and anatomy of three Eucalyptus species: implications for hydraulic conductivity

    Directory of Open Access Journals (Sweden)

    Antonio J. Barotto

    2017-05-01

    Full Text Available Aim of the study: To characterize wood anatomical traits of three Eucalyptus species that differ in wood density and ecological requirements, and to examine the relationships between some anatomical features, wood density, and theoretical xylem hydraulic conductivity (Ks. Area of study: We analyzed 86 trees from three sites of Argentina (Entre Ríos and Buenos Aires Provinces. Methods: The sampled trees were Eucalyptus globulus, E. grandis and E. viminalis ranging from 11 to 15 years old. One stem disc was cut from each tree to determine wood density and identify quantitative anatomical features of vessels and fibers. Vessel composition (S, size - to-number ratio, a measure of vessel size distribution and lumen fraction (F, the total sapwood area available for water transport were estimated. Results: E. grandis, the species with the highest growth rates, presented the highest theoretical Ks. This was associated with anatomical features such as a high density of wide vessels resulting in high F. On the other hand, E. viminalis, the species with the lowest growth rates and highest resistance to environmental stress, showed lower Ks as a result of a low density of wide vessels. These two species differed not only greatly in wood density but also in fiber characteristics. In the case of E. globulus, vessels were relatively narrow, which resulted in the lowest theoretical Ks, fibers were small, and wood density intermediate. Research highlights: F had greater influence on Ks than S. The anatomical characteristics and wood density could only partly explain the differential growth or resistance to stress of the studied species.

  20. Wood density and anatomy of three Eucalyptus species: implications for hydraulic conductivity

    Energy Technology Data Exchange (ETDEWEB)

    Barotto, A.J.; Monteoliva, S.; Gyenge, J.; Martínez-Meier, A.; Moreno, K.; Tesón, N.; Fernández, M.E.

    2017-11-01

    Aim of the study: To characterize wood anatomical traits of three Eucalyptus species that differ in wood density and ecological requirements, and to examine the relationships between some anatomical features, wood density, and theoretical xylem hydraulic conductivity (Ks). Area of study: We analyzed 86 trees from three sites of Argentina (Entre Ríos and Buenos Aires Provinces). Methods: The sampled trees were Eucalyptus globulus, E. grandis and E. viminalis ranging from 11 to 15 years old. One stem disc was cut from each tree to determine wood density and identify quantitative anatomical features of vessels and fibers. Vessel composition (S, size - to-number ratio, a measure of vessel size distribution) and lumen fraction (F, the total sapwood area available for water transport) were estimated. Results: E. grandis, the species with the highest growth rates, presented the highest theoretical Ks. This was associated with anatomical features such as a high density of wide vessels resulting in high F. On the other hand, E. viminalis, the species with the lowest growth rates and highest resistance to environmental stress, showed lower Ks as a result of a low density of wide vessels. These two species differed not only greatly in wood density but also in fiber characteristics. In the case of E. globulus, vessels were relatively narrow, which resulted in the lowest theoretical Ks, fibers were small, and wood density intermediate. Research highlights: F had greater influence on Ks than S. The anatomical characteristics and wood density could only partly explain the differential growth or resistance to stress of the studied species.

  1. COMBUSTION PROPERTIES OF EUCALYPTUS WOOD

    Directory of Open Access Journals (Sweden)

    Yalçın ÖRS

    1999-03-01

    Full Text Available In this study, the combustion properties of some impregnation materials (abiotic and biotic factors used for eucalyptus wood in interior or exterior environments were investigated. The experimental samples were prepared from Eucalyptus wood based on ASTM-D-1413-76 Tanalith-CBC, boric acid, borax, vacsol-WR, immersol-WR, polyethylen glycole-400 and ammonium sulphate were used as an impregnation material. The results indicated that, vacuum treatment on Eucalyptus gave the lowest retention value of salts. Compounds containing boron+salt increased fire resistance however water repellents decreased the wood flammability.

  2. Essential oil composition of Eucalyptus microtheca and Eucalyptus viminalis

    OpenAIRE

    Maghsoodlou, Malek Taher; Kazemipoor, Nasrin; Valizadeh, Jafar; Falak Nezhad Seifi, Mohsen; Rahneshan, Nahid

    2015-01-01

    Objective: Eucalyptus (Fam. Myrtaceae) is a medicinal plant and various Eucalyptus species possess potent pharmacological actions against diabetes, hepatotoxicity, and inflammation. This study aims to investigate essential oil composition from leaves and flowers of E. microtheca and E. viminalis leaves growing in the Southeast of Iran. Materials and Methods: The aerial parts of these plants were collected from Zahedan, Sistan and Baluchestan province, Iran in 2013. After drying the plant mate...

  3. A novel genome-wide microsatellite resource for species of Eucalyptus with linkage-to-physical correspondence on the reference genome sequence.

    Science.gov (United States)

    Grattapaglia, Dario; Mamani, Eva M C; Silva-Junior, Orzenil B; Faria, Danielle A

    2015-03-01

    Keystone species in their native ranges, eucalypts, are ecologically and genetically very diverse, growing naturally along extensive latitudinal and altitudinal ranges and variable environments. Besides their ecological importance, eucalypts are also the most widely planted trees for sustainable forestry in the world. We report the development of a novel collection of 535 microsatellites for species of Eucalyptus, 494 designed from ESTs and 41 from genomic libraries. A selected subset of 223 was evaluated for individual identification, parentage testing, and ancestral information content in the two most extensively studied species, Eucalyptus grandis and Eucalyptus globulus. Microsatellites showed high transferability and overlapping allele size range, suggesting they have arisen still in their common ancestor and confirming the extensive genome conservation between these two species. A consensus linkage map with 437 microsatellites, the most comprehensive microsatellite-only genetic map for Eucalyptus, was built by assembling segregation data from three mapping populations and anchored to the Eucalyptus genome. An overall colinearity between recombination-based and physical positioning of 84% of the mapped microsatellites was observed, with some ordering discrepancies and sporadic locus duplications, consistent with the recently described whole genome duplication events in Eucalyptus. The linkage map covered 95.2% of the 605.8-Mbp assembled genome sequence, placing one microsatellite every 1.55 Mbp on average, and an overall estimate of physical to recombination distance of 618 kbp/cM. The genetic parameters estimates together with linkage and physical position data for this large set of microsatellites should assist marker choice for genome-wide population genetics and comparative mapping in Eucalyptus. © 2014 John Wiley & Sons Ltd.

  4. Normalized cDNA libraries

    Science.gov (United States)

    Soares, Marcelo B.; Efstratiadis, Argiris

    1997-01-01

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

  5. Eucalyptus oil nanoemulsion-impregnated chitosan film: antibacterial effects against a clinical pathogen, Staphylococcus aureus, in vitro

    Directory of Open Access Journals (Sweden)

    Sugumar S

    2015-10-01

    Full Text Available Saranya Sugumar, Amitava Mukherjee, Natarajan Chandrasekaran Centre for Nanobiotechnology, VIT University, Vellore, India Abstract: Eucalyptus oil (Eucalyptus globulus nanoemulsion was formulated using low- and high-energy emulsification methods. Development of nanoemulsion was optimized for system parameters such as emulsifier type, emulsifier concentration, and emulsification methods to obtain a lower droplet size with greater stability. The minimized droplet diameter was achieved using the high-energy method of ultrasonication. Tween 80 was more effective in reducing droplet size and emulsion appearance when compared to Tween 20. Stable nanoemulsion was formulated with Tween 80 as a surfactant, and the particle size was found to be 9.4 nm (1:2 v/v. The eucalyptus oil nanoemulsion was impregnated into chitosan (1% as a biopolymer in varying concentrations. Further, the film was characterized by moisture content, microscopic study, X-ray diffraction, and Fourier transform infrared spectroscopy. Also, the film with and without nanoemulsion was evaluated against Staphylococcus aureus. The nanoemulsion-impregnated chitosan film showed higher antibacterial activity than chitosan film. These results support the inclusion of nanoemulsion-impregnated chitosan film in wound management studies. Keywords: essential oil, emulsion, biopolymer, impregnation, thin film, wound isolate

  6. Effiacy of citronella and eucalyptus oils against Musca domestica, Cimex lectularius and Pediculus humanus

    Directory of Open Access Journals (Sweden)

    Jehangir Khan

    2017-11-01

    Full Text Available Objective: To assess potential impacts of two indigenous plant oils: the citronella (Cymbopogon nardus and eucalyptus (Eucalyptus globulus for their insecticidal effect against Musca domestica (house fly, Cimex lectularius (bed bug and Pediculus humanus (louse. Methods: The oils of these two medicinal plants were applied separately at various concentrations (1, 2 and 3 mL/cup in cups lined with filter paper containing the target insects. Mortality was evaluated after fixed intervals (6, 12 and 24 h subsequent to the release of adult insects. Results: Results showed that both oils exhibited concentration and time dependent mortality against the tested insects. Data pertaining to present investigation clearly showed that percentage mortality owing to these botanicals against these medical pests was significantly high (98.33% at the rate of 3 mL for 24 h of exposure, followed by 2 and 1 mL concentrations with 12 and 6 h of exposure times. Conclusions: The results suggest that these plant oils possess good insecticidal properties against house fly, bed bug, and louse, and are safe to humans. Furthermore, the molecular (biochemical based study of these botanicals against diverse species of pests will be of much significance to control these pest insects.

  7. Oligo-Carrageenan Kappa-Induced Reducing Redox Status and Increase in TRR/TRX Activities Promote Activation and Reprogramming of Terpenoid Metabolism in Eucalyptus Trees

    Directory of Open Access Journals (Sweden)

    Alberto González

    2014-06-01

    Full Text Available In order to analyze whether the reducing redox status and activation of thioredoxin reductase (TRR/thioredoxin(TRX system induced by oligo-carrageenan (OC kappa in Eucalyptus globulus activate secondary metabolism increasing terpenoid synthesis, trees were sprayed on the leaves with water, with OC kappa, or with inhibitors of NAD(PH, ascorbate (ASC and (GSH synthesis and TRR activity, CHS-828, lycorine, buthionine sulfoximine (BSO and auranofine, respectively, and with OC kappa and cultivated for four months. The main terpenoids in control Eucalyptus trees were eucalyptol (76%, α-pinene (7.4%, aromadendrene (3.6%, silvestrene (2.8%, sabinene (2% and α-terpineol (0.9%. Treated trees showed a 22% increase in total essential oils as well as a decrease in eucalyptol (65% and sabinene (0.8% and an increase in aromadendrene (5%, silvestrene (7.8% and other ten terpenoids. In addition, treated Eucalyptus showed seven de novo synthesized terpenoids corresponding to carene, α-terpinene, α-fenchene, γ-maaliene, spathulenol and α-camphenolic aldehyde. Most increased and de novo synthesized terpenoids have potential insecticidal and antimicrobial activities. Trees treated with CHS-828, lycorine, BSO and auranofine and with OC kappa showed an inhibition of increased and de novo synthesized terpenoids. Thus, OC kappa-induced reducing redox status and activation of TRR/TRX system enhance secondary metabolism increasing the synthesis of terpenoids and reprogramming of terpenoid metabolism in Eucalyptus trees.

  8. Oligo-carrageenan kappa-induced reducing redox status and increase in TRR/TRX activities promote activation and reprogramming of terpenoid metabolism in Eucalyptus trees.

    Science.gov (United States)

    González, Alberto; Gutiérrez-Cutiño, Marlen; Moenne, Alejandra

    2014-06-05

    In order to analyze whether the reducing redox status and activation of thioredoxin reductase (TRR)/thioredoxin(TRX) system induced by oligo-carrageenan (OC) kappa in Eucalyptus globulus activate secondary metabolism increasing terpenoid synthesis, trees were sprayed on the leaves with water, with OC kappa, or with inhibitors of NAD(P)H, ascorbate (ASC) and (GSH) synthesis and TRR activity, CHS-828, lycorine, buthionine sulfoximine (BSO) and auranofine, respectively, and with OC kappa and cultivated for four months. The main terpenoids in control Eucalyptus trees were eucalyptol (76%), α-pinene (7.4%), aromadendrene (3.6%), silvestrene (2.8%), sabinene (2%) and α-terpineol (0.9%). Treated trees showed a 22% increase in total essential oils as well as a decrease in eucalyptol (65%) and sabinene (0.8%) and an increase in aromadendrene (5%), silvestrene (7.8%) and other ten terpenoids. In addition, treated Eucalyptus showed seven de novo synthesized terpenoids corresponding to carene, α-terpinene, α-fenchene, γ-maaliene, spathulenol and α-camphenolic aldehyde. Most increased and de novo synthesized terpenoids have potential insecticidal and antimicrobial activities. Trees treated with CHS-828, lycorine, BSO and auranofine and with OC kappa showed an inhibition of increased and de novo synthesized terpenoids. Thus, OC kappa-induced reducing redox status and activation of TRR/TRX system enhance secondary metabolism increasing the synthesis of terpenoids and reprogramming of terpenoid metabolism in Eucalyptus trees.

  9. Immunolocalization of hydrophobin HYDPt-1 from the ectomycorrhizal basidiomycete Pisolithus tinctorius during colonization of Eucalyptus globulus roots

    NARCIS (Netherlands)

    Tagu, D; De Bellis, R; Balestrini, R; De Vries, OMH; Piccoli, G; Stocchi, [No Value; Bonfante, P; Martin, F

    The immunolocalization of one of the hydrophobins of Pisolithus tinctorius (HYDPt-1) is reported. Hydrophobin proteins play key roles in adhesion and aggregation of fungal hyphae, and it is already known that formation of ectomycorrhizas on eucalypt roots enhances the accumulation of hydrophobin

  10. A new genomic resource dedicated to wood formation in Eucalyptus

    Directory of Open Access Journals (Sweden)

    Couloux Arnaud

    2009-03-01

    Full Text Available Abstract Background Renowned for their fast growth, valuable wood properties and wide adaptability, Eucalyptus species are amongst the most planted hardwoods in the world, yet they are still at the early stages of domestication because conventional breeding is slow and costly. Thus, there is huge potential for marker-assisted breeding programs to improve traits such as wood properties. To this end, the sequencing, analysis and annotation of a large collection of expressed sequences tags (ESTs from genes involved in wood formation in Eucalyptus would provide a valuable resource. Results We report here the normalization and sequencing of a cDNA library from developing Eucalyptus secondary xylem, as well as the construction and sequencing of two subtractive libraries (juvenile versus mature wood and vice versa. A total of 9,222 high quality sequences were collected from about 10,000 cDNA clones. The EST assembly generated a set of 3,857 wood-related unigenes including 2,461 contigs (Cg and 1,396 singletons (Sg that we named 'EUCAWOOD'. About 65% of the EUCAWOOD sequences produced matches with poplar, grapevine, Arabidopsis and rice protein sequence databases. BlastX searches of the Uniref100 protein database allowed us to allocate gene ontology (GO and protein family terms to the EUCAWOOD unigenes. This annotation of the EUCAWOOD set revealed key functional categories involved in xylogenesis. For instance, 422 sequences matched various gene families involved in biosynthesis and assembly of primary and secondary cell walls. Interestingly, 141 sequences were annotated as transcription factors, some of them being orthologs of regulators known to be involved in xylogenesis. The EUCAWOOD dataset was also mined for genomic simple sequence repeat markers, yielding a total of 639 putative microsatellites. Finally, a publicly accessible database was created, supporting multiple queries on the EUCAWOOD dataset. Conclusion In this work, we have identified a

  11. Comparative Genomics Analyses Reveal Extensive Chromosome Colinearity and Novel Quantitative Trait Loci in Eucalyptus.

    Directory of Open Access Journals (Sweden)

    Fagen Li

    Full Text Available Dense genetic maps, along with quantitative trait loci (QTLs detected on such maps, are powerful tools for genomics and molecular breeding studies. In the important woody genus Eucalyptus, the recent release of E. grandis genome sequence allows for sequence-based genomic comparison and searching for positional candidate genes within QTL regions. Here, dense genetic maps were constructed for E. urophylla and E. tereticornis using genomic simple sequence repeats (SSR, expressed sequence tag (EST derived SSR, EST-derived cleaved amplified polymorphic sequence (EST-CAPS, and diversity arrays technology (DArT markers. The E. urophylla and E. tereticornis maps comprised 700 and 585 markers across 11 linkage groups, totaling at 1,208.2 and 1,241.4 cM in length, respectively. Extensive synteny and colinearity were observed as compared to three earlier DArT-based eucalypt maps (two maps with E. grandis × E. urophylla and one map of E. globulus and with the E. grandis genome sequence. Fifty-three QTLs for growth (10-56 months of age and wood density (56 months were identified in 22 discrete regions on both maps, in which only one colocalizaiton was found between growth and wood density. Novel QTLs were revealed as compared with those previously detected on DArT-based maps for similar ages in Eucalyptus. Eleven to 585 positional candidate genes were obained for a 56-month-old QTL through aligning QTL confidence interval with the E. grandis genome. These results will assist in comparative genomics studies, targeted gene characterization, and marker-assisted selection in Eucalyptus and the related taxa.

  12. Defense responses in plants of Eucalyptus elicited by Streptomyces and challenged with Botrytis cinerea.

    Science.gov (United States)

    Salla, Tamiris D; Astarita, Leandro V; Santarém, Eliane R

    2016-04-01

    Elicitation of E. grandis plants with Streptomyces PM9 reduced the gray-mold disease, through increasing the levels of enzymes directly related to the induction of plant defense responses, and accumulation of specific phenolic compounds. Members of Eucalyptus are economically important woody species, especially as a raw material in many industrial sectors. Species of this genus are susceptible to pathogens such as Botrytis cinerea (gray mold). Biological control of plant diseases using rhizobacteria is one alternative to reduce the use of pesticides and pathogen attack. This study evaluated the metabolic and phenotypic responses of Eucalyptus grandis and E. globulus plants treated with Streptomyces sp. PM9 and challenged with the pathogenic fungus B. cinerea. Metabolic responses were evaluated by assessing the activities of the enzymes polyphenol oxidase and peroxidase as well as the levels of phenolic compounds and flavonoids. The incidence and progression of the fungal disease in PM9-treated plants and challenged with B. cinerea were evaluated. Treatment with Streptomyces sp. PM9 and challenge with B. cinerea led to changes in the activities of polyphenol oxidase and peroxidase as well as in the levels of phenolic compounds in the plants at different time points. Alterations in enzymes of PM9-treated plants were related to early defense responses in E. grandis. Gallic and chlorogenic acids were on average more abundant, although caffeic acid, benzoic acid and catechin were induced at specific time points during the culture period. Treatment with Streptomyces sp. PM9 significantly delayed the establishment of gray mold in E. grandis plants. These results demonstrate the action of Streptomyces sp. PM9 in inducing plant responses against B. cinerea, making this organism a potential candidate for biological control in Eucalyptus.

  13. Comparative Genomics Analyses Reveal Extensive Chromosome Colinearity and Novel Quantitative Trait Loci in Eucalyptus

    Science.gov (United States)

    Weng, Qijie; Li, Mei; Yu, Xiaoli; Guo, Yong; Wang, Yu; Zhang, Xiaohong; Gan, Siming

    2015-01-01

    Dense genetic maps, along with quantitative trait loci (QTLs) detected on such maps, are powerful tools for genomics and molecular breeding studies. In the important woody genus Eucalyptus, the recent release of E. grandis genome sequence allows for sequence-based genomic comparison and searching for positional candidate genes within QTL regions. Here, dense genetic maps were constructed for E. urophylla and E. tereticornis using genomic simple sequence repeats (SSR), expressed sequence tag (EST) derived SSR, EST-derived cleaved amplified polymorphic sequence (EST-CAPS), and diversity arrays technology (DArT) markers. The E. urophylla and E. tereticornis maps comprised 700 and 585 markers across 11 linkage groups, totaling at 1,208.2 and 1,241.4 cM in length, respectively. Extensive synteny and colinearity were observed as compared to three earlier DArT-based eucalypt maps (two maps with E. grandis × E. urophylla and one map of E. globulus) and with the E. grandis genome sequence. Fifty-three QTLs for growth (10–56 months of age) and wood density (56 months) were identified in 22 discrete regions on both maps, in which only one colocalizaiton was found between growth and wood density. Novel QTLs were revealed as compared with those previously detected on DArT-based maps for similar ages in Eucalyptus. Eleven to 585 positional candidate genes were obained for a 56-month-old QTL through aligning QTL confidence interval with the E. grandis genome. These results will assist in comparative genomics studies, targeted gene characterization, and marker-assisted selection in Eucalyptus and the related taxa. PMID:26695430

  14. Effect of eucalyptus ( Eucalyptus camaldulensis ) and maize (Zea ...

    African Journals Online (AJOL)

    However eucalyptus plantations produce large amounts of litter, which impact on groundnut has not been clearly elucidated yet. In order to investigate litter accumulation effect on growth, development, and groundnut root infection by arbuscular mycorrhizal fungi (AMF) and rhizobia, a greenhouse experiment was performed ...

  15. Eucalyptus as a landscape tree

    Science.gov (United States)

    W. Douglas Hamilton

    1983-01-01

    Ninety-two species of Eucalyptus were evaluated at the University of California re- search station in San Jose. The purpose: to find acceptable new street and park trees. Growth rates and horticultural characteristics were noted. Forty-three species were studied in locations statewide to evaluate site adaptation and landscape usefulness; flooded, cold, dry, saline....

  16. Quantification and economic valuation of the capture of CO2 for Eucalyptus plantations, established by the Preca in the carboniferous Basins of Cesar, Cauca Valley, Cauca and Highland Cundiboyacense

    International Nuclear Information System (INIS)

    Diaz Fonseca, Sandra Ximena; Molano Morales, Miguel Angel

    2001-01-01

    This study, as first measure it looks for to quantify the tons of CO 2 captured by the increment in the biomass of forestall plantations of the Eucalyptus genus established by the PRECA of Ecocarbon in the carboniferous basins of the Cesar, Cauca Valley, Cauca and Highland Cundiboyacense and in second measure to determine the economic value that the sale of this environmental service can represent for a developing country as Colombia. The results obtained for each one of the plantations in each carboniferous basin are determined, and statistical models that will allow calculating the capture of CO 2 carried out by plantations of three different species of Eucalyptus (E. camaldulensis, e. grandis and E. globulus), starting from the volume in foot of the timber only barked

  17. Quantification and economic valuation of the capture of CO2 for plantations of the Eucalyptus, genus, settled down by the PRECA in the carboniferous basins of Cesar, Cauca Valley, Cauca and Cundiboyacense Highland

    International Nuclear Information System (INIS)

    Diaz F, Sandra Ximena; Molano M, Miguel Angel

    2001-01-01

    In this study, the first measure is to quantify the tons of CO 2 captured by the increment in the biomass of forest plantations of the Eucalyptus genus, settled down by the PRECA of Ecocarbon in the carboniferous basins of the Cesar, Cauca Valley, Cauca and Cundiboyacense highland and in second measure to determine the economic value that the sale of this environmental service can represent for a developing country as Colombia. The results obtained for each one of the plantations settled down in each carboniferous basin are determined and statistical models that will allow to calculate the capture of CO 2 carried out by plantations of three different species of Eucalyptus (E. Camaldulensis, E. grandis and E. globulus)

  18. Wood Permeability in Eucalyptus grandis and Eucalyptus dunnii

    Directory of Open Access Journals (Sweden)

    Raphael Nogueira Rezende

    2017-12-01

    Full Text Available ABSTRACT The objective of this study was to evaluate the flow of air and water in Eucalyptus grandis and Eucalyptus dunnii wood. Wood was collected from four trees aged 37 years in an experimental plantation of the Federal University of Lavras, Brazil. Planks were cut off the basal logs to produce specimens for air and water permeability testing. Results indicated that the longitudinal permeability to air and water of E. grandis wood were, on average, 5% and 10% higher, respectively, than that of E. dunnii wood. E. grandis and E. dunnii wood showed neither air nor water flow in the test for permeability transversal to the fibers, and longitudinal permeability to air exceeded that to water by approximately 50 fold in both species.

  19. Breeding Eucalyptus for disease resistance

    Science.gov (United States)

    Edival A.V. Zauza; Acelino Couto Alfenas; Lúcio Mauro da Silva Guimarães; João Flávio da Silva

    2012-01-01

    Eucalyptus plantations cover about 1.5 percent of the agricultural area in Brazil, and contribute to 4 percent of GDP and 3 percent in exports of forest products. Technological and research advances in silviculture and genetic improvement have increased productivity up to 80 m³ of wood/ha/year, with an average of 35 to 45 m³/ha/year. The greatest...

  20. Responses to mild water deficit and rewatering differ among secondary metabolites but are similar among provenances within Eucalyptus species.

    Science.gov (United States)

    McKiernan, Adam B; Potts, Brad M; Brodribb, Timothy J; Hovenden, Mark J; Davies, Noel W; McAdam, Scott A M; Ross, John J; Rodemann, Thomas; O'Reilly-Wapstra, Julianne M

    2016-02-01

    Water deficit associated with drought can severely affect plants and influence ecological interactions involving plant secondary metabolites. We tested the effect of mild water deficit and rewatering on physiological, morphological and chemical traits of juvenile Eucalyptus globulus Labill. and Eucalyptus viminalis Labill. We also tested if responses of juvenile eucalypts to water deficit and rewatering varied within species using provenances across a rainfall gradient. Both species and all provenances were similarly affected by mild water deficit and rewatering, as only foliar abscisic acid levels differed among provenances during water deficit. Across species and provenances, water deficit decreased leaf water potential, above-ground biomass and formylated phloroglucinol compound concentrations, and increased condensed tannin concentrations. Rewatering reduced leaf carbon : nitrogen, and total phenolic and chlorogenic acid concentrations. Water deficit and rewatering had no effect on total oil or individual terpene concentrations. Levels of trait plasticity due to water deficit and rewatering were less than levels of constitutive trait variation among provenances. The overall uniformity of responses to the treatments regardless of native provenance indicates limited diversification of plastic responses when compared with the larger quantitative variation of constitutive traits within these species. These responses to mild water deficit may differ from responses to more extreme water deficit or to responses of juvenile/mature eucalypts growing at each locality. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  1. Thermodynamic Study of Adsorption of Phenol, 4-Chlorophenol, and 4-Nitrophenol on Activated Carbon Obtained from Eucalyptus Seed

    Directory of Open Access Journals (Sweden)

    Nelson Giovanny Rincón-Silva

    2015-01-01

    Full Text Available Activated carbons from shell eucalyptus (Eucalyptus globulus were prepared by chemical activation through impregnation with solutions of two activators: sulfuric acid and sodium hydroxide, the surface areas for activated carbons with base were 780 and 670 m2 g−1 and the solids activated with acid were 150 and 80 m2 g−1. These were applying in adsorption of priority pollutants: phenol, 4-nitrophenol, and 4-chlorophenol from aqueous solution. Activated carbon with the highest adsorption capacity has values of 2.12, 2.57, and 3.89 on phenol, 4-nitrophenol, and 4-chlorophenol, respectively, and was activated with base. In general, all carbons adsorption capacity was given in the following order: 4-chlorophenol > 4-nitrophenol > phenol. Adsorption isotherms of phenols on activated carbons were fitted to the Langmuir, Freundlich, and Dubinin-Radusckevisch-Kanager models, finding great association between them and experimental data. A thermodynamic study was performed, the exothermic nature and spontaneous nature of the adsorption process were confirmed, and the favorability of adsorption on activated carbons with NaOH was confirmed by energy relations and concluded that the adsorption process of phenolic compounds from the activated carbon obtained is physical. The pH of solutions and pH at point of zero charge of the solid play an important role in the adsorption process.

  2. Aggregate stability in soils cultivated with eucalyptus

    Science.gov (United States)

    Eucalyptus cultivation has increased in many Brazilian regions. In order to recommend good management practices, it is necessary to understand changes in soil properties where eucalyptus is planted. Aggregate stability analyses have proved to be a useful tool to measure soil effects caused by change...

  3. Early selection of Eucalyptus clones in retrospective nursery test ...

    African Journals Online (AJOL)

    Within the framework of the eucalyptus breeding programme in the Congo, two retrospective tests were conducted using mature clones in the field and young cuttings under nursery conditions with two hybrids: 13 clones of Eucalyptus tereticornis* Eucalyptus grandis for the test TC 82-1B and 17 clones of Eucalyptus ...

  4. Essential oil composition of Eucalyptus microtheca and Eucalyptus viminalis

    Directory of Open Access Journals (Sweden)

    Malek Taher Maghsoodlou

    2015-10-01

    Full Text Available Objective: Eucalyptus (Fam. Myrtaceae is a medicinal plant and various Eucalyptus species possess potent pharmacological actions against diabetes, hepatotoxicity, and inflammation. This study aims to investigate essential oil compositionfrom leaves and flowers of E. microthecaand E. viminalisleavesgrowing in the Southeast of Iran. Materials and Methods: The aerial parts of these plants were collected from Zahedan, Sistan and Baluchestan province, Iran in 2013.After drying the plant materials in the shade, the chemical composition of the essential oils was obtained by hydro-distillation method using a Clevenger-type apparatus and analyzed by GC/MS. Results: In the essential oil of E. microtheca leaves, 101 compounds representing 100%, were identified. Among them, α-phellandrene (16.487%, aromadendrene (12.773%, α-pinene (6.752%, globulol (5.997%, ledene (5.665%, P-cymen (5.251%, and β-pinene (5.006% were the major constituents.  In the oil of E. microtheca flowers, 88 compounds representing 100%, were identified in which α-pinene (16.246%, O-cymen (13.522%, β-pinene (11.082%, aromadendrene (7.444%, α-phellandrene (7.006%, globulol (5.419%, and 9-octadecenamide (5.414% were the major components. Sixty six compounds representing 100% were identified in the oil of E. viminalis leaves. The major compounds were 1, 8-cineole (57.757%, α-pinene (13.379%, limonene (5.443%, and globulol (3.054%. Conclusion: The results showed the essential oils fromthe aerial parts of Eucalyptus speciesare a cheap source for the commercial isolation of α-phellandrene, α-pinene, and 1, 8-cineole compounds to be used in medicinal and food products. Furthermore, these plants could be an alternative source of insecticide agents.

  5. Essential oil composition of Eucalyptus microtheca and Eucalyptus viminalis.

    Science.gov (United States)

    Maghsoodlou, Malek Taher; Kazemipoor, Nasrin; Valizadeh, Jafar; Falak Nezhad Seifi, Mohsen; Rahneshan, Nahid

    2015-01-01

    Eucalyptus (Fam. Myrtaceae) is a medicinal plant and various Eucalyptus species possess potent pharmacological actions against diabetes, hepatotoxicity, and inflammation. This study aims to investigate essential oil composition from leaves and flowers of E. microtheca and E. viminalis leaves growing in the Southeast of Iran. The aerial parts of these plants were collected from Zahedan, Sistan and Baluchestan province, Iran in 2013. After drying the plant materials in the shade, the chemical composition of the essential oils was obtained by hydro-distillation method using a Clevenger-type apparatus and analyzed by GC/MS. In the essential oil of E. microtheca leaves, 101 compounds representing 100%, were identified. Among them, α-phellandrene (16.487%), aromadendrene (12.773%), α-pinene (6.752%), globulol (5.997%), ledene (5.665%), P-cymen (5.251%), and β-pinene (5.006%) were the major constituents. In the oil of E. microtheca flowers, 88 compounds representing 100%, were identified in which α-pinene (16.246%), O-cymen (13.522%), β-pinene (11.082%), aromadendrene (7.444%), α-phellandrene (7.006%), globulol (5.419%), and 9-octadecenamide (5.414%) were the major components. Sixty six compounds representing 100% were identified in the oil of E. viminalis leaves. The major compounds were 1, 8-cineole (57.757%), α-pinene (13.379%), limonene (5.443%), and globulol (3.054%). The results showed the essential oils from the aerial parts of Eucalyptus species are a cheap source for the commercial isolation of α-phellandrene, α-pinene, and 1, 8-cineole compounds to be used in medicinal and food products. Furthermore, these plants could be an alternative source of insecticide agents.

  6. Eucalyptus gunnii: A possible source of bioenergy?

    Energy Technology Data Exchange (ETDEWEB)

    Forrest, Mary; Moore, Tom [UCD School of Biology and Environmental Science, Agriculture and Food Science Centre, University College Dublin, Belfield, Dublin 4 (Ireland)

    2008-10-15

    Eucalyptus are widely grown throughout the world for timber, essential oil, fuel, biomass and cut foliage. In experiments in University College Dublin (UCD), Eucalyptus gunnii yielded an average dry weight of 4.74 kg per plant per annum, equivalent to 12.59 t dm ha{sup -1}. The average calorific values of 17.60 MJ kg{sup -1} (leafy material) and 17.09 MJ kg{sup -1} (woody material) are less than the values reported for other species of Eucalyptus. (author)

  7. Insect pests of Eucalyptus and their control

    Energy Technology Data Exchange (ETDEWEB)

    Sen-Sarma, P K; Thakur, M L

    1983-12-01

    In India, about sixty odd species of insects have so far been recorded to be associated with Eucalyptus. Important pests are some xylophagous insects, sap suckers, defoliators and termites. Of these, stem and root borer, Celostrna scabrator Fabr, and some species of termites have been recognised as key pests, whereas Apogonia coriaces Waterhouse, Mimeta mundissima Walker (Coleoptera: Scarabaeidae), Agrotis ipsilon Hufnagel (Lepidoptera: Noctuidae), Brachytrypus portenosus Lichtenstein and Gymmogryllus humeralis Walker (Orthoptera: Gryllidae) are likely to become potential pests in Eucalyptus nurseries. In this paper available information on insect pests of Eucalyptus, their bioecology and control measures have been presented.

  8. Isolation of intact sub-dermal secretory cavities from Eucalyptus

    Directory of Open Access Journals (Sweden)

    Goodger Jason QD

    2010-09-01

    Full Text Available Abstract Background The biosynthesis of plant natural products in sub-dermal secretory cavities is poorly understood at the molecular level, largely due to the difficulty of physically isolating these structures for study. Our aim was to develop a protocol for isolating live and intact sub-dermal secretory cavities, and to do this, we used leaves from three species of Eucalyptus with cavities that are relatively large and rich in essential oils. Results Leaves were digested using a variety of commercially available enzymes. A pectinase from Aspergillus niger was found to allow isolation of intact cavities after a relatively short incubation (12 h, with no visible artifacts from digestion and no loss of cellular integrity or cavity contents. Several measurements indicated the potential of the isolated cavities for further functional studies. First, the cavities were found to consume oxygen at a rate that is comparable to that estimated from leaf respiratory rates. Second, mRNA was extracted from cavities, and it was used to amplify a cDNA fragment with high similarity to that of a monoterpene synthase. Third, the contents of the cavity lumen were extracted, showing an unexpectedly low abundance of volatile essential oils and a sizeable amount of non-volatile material, which is contrary to the widely accepted role of secretory cavities as predominantly essential oil repositories. Conclusions The protocol described herein is likely to be adaptable to a range of Eucalyptus species with sub-dermal secretory cavities, and should find wide application in studies of the developmental and functional biology of these structures, and the biosynthesis of the plant natural products they contain.

  9. First insights into the functional role of vasicentric tracheids and parenchyma in eucalyptus species with solitary vessels: do they contribute to xylem efficiency or safety?

    Science.gov (United States)

    Barotto, Antonio José; Fernandez, María Elena; Gyenge, Javier; Meyra, Ariel; Martinez-Meier, Alejandro; Monteoliva, Silvia

    2016-12-01

    The relationship between hydraulic specific conductivity (k s ) and vulnerability to cavitation (VC) with size and number of vessels has been studied in many angiosperms. However, few of the studies link other cell types (vasicentric tracheids (VT), fibre-tracheids, parenchyma) with these hydraulic functions. Eucalyptus is one of the most important genera in forestry worldwide. It exhibits a complex wood anatomy, with solitary vessels surrounded by VT and parenchyma, which could serve as a good model to investigate the functional role of the different cell types in xylem functioning. Wood anatomy (several traits of vessels, VT, fibres and parenchyma) in conjunction with maximum k s and VC was studied in adult trees of commercial species with medium-to-high wood density (Eucalyptus globulus Labill., Eucalyptus viminalis Labill. and Eucalyptus camaldulensis Dehnh.). Traits of cells accompanying vessels presented correlations with functional variables suggesting that they contribute to both increasing connectivity between adjacent vessels-and, therefore, to xylem conduction efficiency-and decreasing the probability of embolism propagation into the tissue, i.e., xylem safety. All three species presented moderate-to-high resistance to cavitation (mean P 50 values = -2.4 to -4.2 MPa) with no general trade-off between efficiency and safety at the interspecific level. The results in these species do not support some well-established hypotheses of the functional meaning of wood anatomy. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  10. A COMPARATIVE STUDY ON MACROSSOPIC, MICROSCOPIC AND PHYTOCHEMICAL PROPERTIES OF EUCALYPTUS SPEJES, SPEJES, CULTIVATED IN IRAN, WITH STANDARD SPECIES

    Directory of Open Access Journals (Sweden)

    samsam shariat sadeghi-aliabadi

    1993-07-01

    Full Text Available The aim of this study is to compare the volatile oil of staendard species of Eucalyptus with those species cultivated in Iran. The investigation was carried out as bellow:"nA The seeds of elven species were supplied from Germany and cultivated in Research Center of Agriculture of Isfahan and then extracted for volatile oil and cineole."nB Besides, we supplied 2 species from Isfahan, 7 species from Fars province, 9 from Khuzestan province, one from Tehran and one from Hormozgan, province."nC We extracted total volatile oil of 28 E. species, then injected to G. G. column and the results were as follow:"n1 - In the following species : E. saligna, E. loxophleba and E. Stratycalyx from Esfahan province. E."nloxophleba, E.camaldulensis, E.stratycalyx, intertexta and E. caleicultrix from Fars"nprovince. E. Stratycalyx, E. OCCidsntalis, E. gillii and E. sargentii from khozestan prcvince. E. globulus from Tehran and E. camaldulensis from Hormozgan province. The amount of essential oil extracted from the mentioned species was in accord with the standard of pharmacopial level. But the extracted oil from others was not as standard levl."n2 - E. gillii, E. camaldulensis, E. sargentii and OCCidentalis from khouzestan and the species of"nE.intertexta, E. camaldulensis, E. lansdownean from fars and the species of E. camaldulensis, E. saligna from Esfahan and the species of E. globulus from Tehran, the amount of measured cineol was in accordance the standard pharmacopeial level."n3 - Some of the species studied such as : were qualified for the amount of Alpha and Beta pinen.

  11. Marker-assisted selection in Eucalyptus

    International Nuclear Information System (INIS)

    Grattapaglia, D.

    2007-01-01

    Planted Eucalyptus occupies globally more than 18 million hectares and has become the most widely planted hardwood tree in the world, supplying high-quality woody biomass for several industrial applications. In this chapter an overview is presented on the status and perspectives of marker-assisted selection (MAS) in species of Eucalyptus. After an introduction to the main features of modern eucalypt breeding and clonal forestry, some applications of molecular markers in support to operational breeding are presented. By reviewing the status of quantitative trait locus (QTL) mapping in Eucalyptus, the challenges and some realistic prospects for the application of MAS to improve relevant traits are outlined. With the expected availability of more powerful genomic tools, including a draft of the Eucalyptus genome, the main challenges in implementing MAS will be in phenotyping trees accurately, analysing the overwhelming amount of genomic data available and translating this into truly useful molecular tools for breeding. (author)

  12. The floral transcriptome of Eucalyptus grandis

    CSIR Research Space (South Africa)

    Vining, KJ

    2015-10-01

    Full Text Available As a step toward functional annotation of genes required for floral initiation and development within the Eucalyptus genome, we used short read sequencing to analyze transcriptomes of floral buds from early and late developmental stages...

  13. Potential of eleven Eucalyptus species for the production of essential oils Potencial de onze espécies de eucalipto para a produção de óleos essenciais

    Directory of Open Access Journals (Sweden)

    Paulo Henrique Müller da Silva

    2006-02-01

    Full Text Available Most Eucalyptus plantations in Brazil aim the production of paper and charcoal, but the use of the species for lumbering, construction and extraction of essential oil has increased. Eleven species of Eucalyptus were assessed in regard to their essential oil production potential, nine never used before for commercial, essential oil extraction. Assessements were compared with Eucalyptus citriodora and Eucalyptus globulus, already explored in oil production for perfume and medical purposes, aiming to increase the availability of commercial species and the relationship between oil production and climatic conditions. Eucalyptus leaves were seasonally sampled (three months interval for distillation and determination of productivity and chemical composition of oil - contents of oils main components. Eucalyptus viminalis showed the highest potential for cineol, and standed out among the species not yet used commercially. Influenced by soil moisture contents and air temperature, the lowest and the highest oil production happened respectively in Spring and Summer. Water deficiency in Spring caused the lowest oil production. In the Summer, on the other hand, the highest oil production was observed when temperatures were higher and no water deficiency registered. There was no climatic influence on the main oil chemical component.A maioria das plantações de Eucalyptus brasileiras tem como objetivo a produção de papel e carvão, mas o uso das espécies para madeira, construção e extração de óleo essencial tem aumentado. Neste trabalho foram estudadas onze espécies de eucalipto quanto ao potencial para a obtenção de óleo essencial. Entre as espécies estudadas, nove não têm sido utilizadas na obtenção comercial de óleo. Para efeito comparativo, avaliou-se também duas outras espécies, o Eucalyptus citriodora e o Eucalyptus globulus, as quais são amplamente utilizadas, respectivamente, para a obtenção de óleo aromatizante e óleo para

  14. Regeneration and transformation of Eucalyptus grandis

    OpenAIRE

    Esteki, Leila

    2013-01-01

    Dissertação de mestrado em Biologia Molecular, Biotecnologia e Bioempreendedorismo em Plantas Eucalyptus grandis is the most widely used species in planted forests in tropical and subtropical areas. The traits of interest underlying Eucalyptus breeding programs concern productivity and wood quality for the pulp and paper industry, as well as biotic and abiotic stress tolerance. The development of an efficient transformation protocol is necessary to explore eucalypt resources th...

  15. Effects of Allelochemicals of Some Eucalyptus Species on ...

    African Journals Online (AJOL)

    A laboratory experiment was conducted to assess the effects of allelochemicals of Eucalyptus camaldulensis, Eucalyptus citriodora and Eucalyptus globules on germination and root elongation using leguminous crop ground nut (Arachis hypogea) as bioassay material. The experiments were conducted in sterilized ...

  16. Profitability of Eucalyptus growing in Busiro, Mpigi District, Uganda

    African Journals Online (AJOL)

    supply of woody products from the existing forest resources is decreasing. ... Key words: Household level investments; Eucalyptus; financial profitability. ... eucalyptus (kalitunsi) tree species to other forest tree ... financial analyses is an assumption that market prices eucalyptus woodlot is situated in ... not be of good quality.

  17. TANNIN CONTENT DETERMINATION IN THE BARK OF Eucalyptus spp

    Directory of Open Access Journals (Sweden)

    Paulo Fernando Trugilho

    2003-07-01

    Full Text Available The objective of this study was to determine the tannin contents in the bark oftwenty-five species of Eucalyptus through two extraction methods, one using hot water andthe other a sequence of toluene and ethanol. The results showed that the extraction methodspresented significant differences in the tannin contents. The method using the sequencetoluene and ethanol, for most of the species, promoted a larger extraction of tannin. The hotwater method presented higher contents of tannin for Eucalyptus cloeziana (40,31%,Eucalyptus melanophoia (20,49% and Eucalyptus paniculata (16,03%. In the toluene andethanol method the species with higher tannin content was Eucalyptus cloeziana (31,00%,Eucalyptus tereticornis (22,83% and Eucalyptus paniculata (17,64%. The Eucalyptuscloeziana presented great potential as commercial source of tannin, independent of theextraction method considered.

  18. Botanical, Phytochemical, and Anticancer Properties of the Eucalyptus Species.

    Science.gov (United States)

    Vuong, Quan V; Chalmers, Anita C; Jyoti Bhuyan, Deep; Bowyer, Michael C; Scarlett, Christopher J

    2015-06-01

    The genus Eucalyptus (Myrtaceae) is mainly native to Australia; however, some species are now distributed globally. Eucalyptus has been used in indigenous Australian medicines for the treatment of a range of aliments including colds, flu, fever, muscular aches, sores, internal pains, and inflammation. Eucalyptus oils containing volatile compounds have been widely used in the pharmaceutical and cosmetics industries for a multitude of purposes. In addition, Eucalyptus extracts containing nonvolatile compounds are also an important source of key bioactive compounds, and several studies have linked Eucalyptus extracts with anticancer properties. With the increasing research interest in Eucalyptus and its health properties, this review briefly outlines the botanical features of Eucalyptus, discusses its traditional use as medicine, and comprehensively reviews its phytochemical and anticancer properties and, finally, proposes trends for future studies. Copyright © 2015 Verlag Helvetica Chimica Acta AG, Zürich.

  19. Clonal propagation of eucalyptus by tissue culture

    Energy Technology Data Exchange (ETDEWEB)

    Mehra-Palta, A.

    1982-07-01

    Multiple adventitious buds were induced on cotyledons, shoot tips and nodal stem segments of Eucalyptus species cultured on a defined nutrient medium supplemented with the cytokinin zeatin and the auxin indole-3-butyric acid (IBA). The adventitious buds could be recycled on cytokinin medium to produce more buds thus providing the possibility of producing large clones from selected genotypes. The adventitious shoots were rooted in auxin medium and some of the resulting propagules were outplanted in the field. These techniques have the potential for use in the genetic improvement of Eucalyptus. (Refs. 15).

  20. Effects of Treatment Materials on the Physical Properties of Eucalyptus (Eucalyptus camaldulensis Dehn. Wood

    Directory of Open Access Journals (Sweden)

    Selahattin Bardak

    2011-04-01

    Full Text Available This study was designed to determine effects on retention and shrink levels of Eucalyptus (Eucalyptus camaldulensis Dehn. which treated with some commercial preservative types, borates, polyethylene glycol, and water repellents. In this study, four type impregnation chemicals were chosen: 1. Commercial preservative types, [Ammonium sulphate, Vacsol-WR WR and Immersol-WR (WR], 2. Borates chemicals, [Boric acid, Borax, Boric acid+ Borax] 3. Polyethylene glycol such as Polyethylene glycol (PEG-400 4. Water repellents [Styrene, Methylmetacrylate.]. As a result, retention % and shrink levels of Eucalyptus wood was lower treated with commercial preservative types and borates preservatives compare to other treatment chemicals. However, retention % levels of Eucalyptus wood treated with WR chemicals were highly significant levels. According to their leachability period although PEG 400 showed antishrink effectiveness other treatments didnt showed antishrink efficiency.

  1. Actinomycetes from Eucalyptus and their biological activities for controlling Eucalyptus leaf and shoot blight.

    Science.gov (United States)

    Himaman, Winanda; Thamchaipenet, Arinthip; Pathom-Aree, Wasu; Duangmal, Kannika

    2016-01-01

    In Thailand, Eucalyptus plantations rapidly expand across the country. Leaf and shoot blight caused by Cryptosporiopsis eucalypti, Cylindrocladium sp. and Teratosphaeria destructans is a serious disease in Eucalyptus plantations. In this study, a total of 477 actinomycete strains were successfully isolated from roots and rhizosphere soil of Eucalyptus. Four hundred and thirty nine isolates were classified as streptomycetes and 38 isolates were non-streptomycetes. Among these isolates, 272 (57.0%), 118 (24.7%) and 241 (50.5%) isolates were antagonistic to Cryptosporiopsis eucalypti, Cylindrocladium sp. and Teratosphaeria destructans, respectively. All isolates were tested for their abilities to produce siderophores, indole acetic acid (IAA) and solubilise phosphate. Most isolates (464, 97.3%) produced siderophores. The majority of isolates (345, 72.3%) solubilised phosphate. In addition, almost half of these isolates (237, 49.7%) produced indole acetic acid. Strain EUSKR2S82 which showed the strongest inhibitory effect against all tested fungi with plant growth promoting ability was selected to test with Eucalyptus. This strain could colonize plant roots and increase Eucalyptus roots length. In a detached leaves bioassay, the disease severity of EUSKR2S82-inoculated Eucalyptus leaves was only 30% compared to 95% in the control treatment. The 16S rRNA gene sequence analysis revealed that the strain EUSKR2S82 was related to Streptomyces ramulosus NRRL-B 2714(T) (99.44% similarity). Identification of non-streptomycete isolates using 16S rRNA gene sequences classified them into 9 genera: Actinoallomurus, Actinomadura, Amycolatopsis, Cryptosporangium, Microbispora, Micromonospora, Nocardia, Nonomuraea and Pseudonocardia. It is evident that Eucalyptus tree harbored several genera of actinomycetes. The selected isolate, EUSKR2S82 showed potential as a candidate for biocontrol agent of leaf and shoot blight of Eucalyptus and to promote growth. Copyright © 2016 Elsevier Gmb

  2. PCR-based cDNA library construction: general cDNA libraries at the level of a few cells.

    OpenAIRE

    Belyavsky, A; Vinogradova, T; Rajewsky, K

    1989-01-01

    A procedure for the construction of general cDNA libraries is described which is based on the amplification of total cDNA in vitro. The first cDNA strand is synthesized from total RNA using an oligo(dT)-containing primer. After oligo(dG) tailing the total cDNA is amplified by PCR using two primers complementary to oligo(dA) and oligo(dG) ends of the cDNA. For insertion of the cDNA into a vector a controlled trimming of the 3' ends of the cDNA by Klenow enzyme was used. Starting from 10 J558L ...

  3. Variación genética en plántulas de Eucalyptus spp.

    Directory of Open Access Journals (Sweden)

    H. C. Martínez Hernández

    2001-01-01

    Full Text Available En este estudio se evaluó el crecimiento y acumulación de biomasa en plántulas de 7 meses de edad pertenecientes a 42 familias de polinización libre de tres especies de Eucalyptus (E. globulus, E. resinifera, y E. rudis. El ensayo se estableció en condiciones de vivero con germoplasma colectado en plantaciones establecidas en la cuenca del Valle de México. Los resultados indicaron la existencia de variación significativa (P≤0.05 entre especies y entre familias dentro de especies para las variables altura, diámetro, número de hojas y número de ramas, aunque no en el área foliar. A nivel de especies se encontraron diferencias significativas en todas las variables relacionadas con la acumulación y distribución de biomasa, excepto en el peso seco de las hojas. También se encontró una variación significativa (P≤0.01 a nivel de familias dentro de especies en el peso seco del tallo, el peso seco de hojas y la biomasa aérea mientras que el peso seco de las ramas y la biomasa de raíz no presentaron una variación significativa entre las familias. El componente de variación de familias dentro de especies aportó del 2 al 12 % de la variación fenotípica total, mientras que las especies contribuyeron sólo del 0.5 a 7.5 de esta variación. El mayor porcentaje de variación se presentó dentro de parcelas, con valores de 80 a 90 % del total en todas las variables de crecimiento y acumulación de biomasa de las plántulas.

  4. Growth potential limits drought morphological plasticity in seedlings from six Eucalyptus provenances.

    Science.gov (United States)

    Maseda, Pablo H; Fernández, Roberto J

    2016-02-01

    Water stress modifies plant above- vs belowground biomass allocation, i.e., morphological plasticity. It is known that all species and genotypes reduce their growth rate in response to stress, but in the case of water stress it is unclear whether the magnitude of such reduction is linked to the genotype's growth potential, and whether the reduction can be largely attributed to morphological adjustments such as plant allocation and leaf and root anatomy. We subjected seedlings of six seed sources, three from each of Eucalyptus camaldulensis (potentially fast growing) and E. globulus (inherently slow growing), to three experimental water regimes. Biomass, leaf area and root length were measured in a 6-month glasshouse experiment. We then performed functional growth analysis of relative growth rate (RGR), and aboveground (leaf area ratio (LAR), specific leaf area (SLA) and leaf mass ratio (LMR)) and belowground (root length ratio (RLR), specific root length (SRL) and root mass ratio (RMR)) morphological components. Total biomass, root biomass and leaf area were reduced for all Eucalyptus provenances according to drought intensity. All populations exhibited drought plasticity, while those of greater growth potential (RGRmax) had a larger reduction in growth (discounting the effect of size). A positive correlation was observed between drought sensitivity and RGRmax. Aboveground, drought reduced LAR and LMR; under severe drought a negative correlation was found between LMR and RGRmax. Belowground, drought reduced SRL but increased RMR, resulting in no change in RLR. Under severe drought, a negative correlation was found between RLR, SRL and RGRmax. Our evidence strongly supports the classic ecophysiological trade-off between growth potential and drought tolerance for woody seedlings. It also suggests that slow growers would have a low capacity to adjust their morphology. For shoots, this constraint on plasticity was best observed in partition (i.e., LMR) whereas for

  5. Managing a Coastal Bluegum (Eucalyptus globules) forest

    Science.gov (United States)

    Ralph S. Osterling

    1983-01-01

    Eucalyptus was thought to be a replacement to oak and other hardwoods for many products. Thousands of acres have been planted and now are in need of management. Management techniques are discussed in context with a coastal stand of approximately 300 acres of mixed aged bluegum. Potential markets are explored.

  6. Determination of pectin content of eucalyptus wood

    NARCIS (Netherlands)

    Coetzee, B.; Schols, H.A.; Wolfaardt, F.

    2011-01-01

    Very little is known about the occurrence of pectin in wood and it is speculated that between 10 mg g-1 and 40 mg g-1 of wood consists of pectin. The present study aimed to quantify pectin in eucalyptus wood and to determine the influence of tree species, yield potential of the site, tree age class

  7. Allelopathic effects of invasive Eucalyptus camaldulensis on ...

    African Journals Online (AJOL)

    Eucalyptus camaldulensis Dehnh. (red river gum; Myrtaceae) is an invasive tree in riparian habitats of the Western Cape, South Africa, where it replaces indigenous vegetation and affects ecosystem functioning. These invasions lead to changes in river geomorphology and reduction in stream flow. The mechanisms that ...

  8. Toxicidad, efecto antialimentario y repelente de metabolitos secundarios de Eucalyptus globulus (Labill) (Myrtaceae) sobre coleópteros de importancia agrícola

    OpenAIRE

    Russo, Serafina

    2013-01-01

    Las plantas aromáticas y sus aceites esenciales han sido utilizados desde la antigüedad como condimento, por su sabor y aroma; también como agente antimicrobiano e insecticida, para repeler insectos y/o para proteger los productos almacenados. Constituyen eficaces alternativas a los plaguicidas sintéticos convencionales de amplio espectro con baja toxicidad para el medio ambiente, mayor biodegradabilidad y baja generación de resistencia. El objetivo de este trabajo fue: I) analizar la composi...

  9. Near-surface distributions of soil water and water repellency under three effluent irrigation scemes in a blue gum (Eucalyptus globulus) plantation

    NARCIS (Netherlands)

    Thwaites, L.A.; Rooij, de G.H.; Salzman, S.; Allinson, G.; Stagnitti, F.; Carr, R.; Versace, V.; Struck, S.; March, T.

    2006-01-01

    Water repellent soils are difficult to irrigate and susceptible to preferential flow, which enhances the potential for accelerated leaching to groundwater of hazardous substances. Over 5 Mha of Australian soil is water repellent, while treated municipal sewage is increasingly used for irrigation.

  10. Soil and dasometric characterization of a Eucalyptus globulus Labill plantation and management proposal in the lower montane thorny steppe zone, Riobamba, Ecuador

    Directory of Open Access Journals (Sweden)

    Miguel Guallpa

    2016-09-01

    Full Text Available There is limited information on the growth and yield of the Tasmanian blue gum plantation in Tunshi-ESPOCH homestead in varying physiographic conditions, soil properties and management; for this reason it was decided to assess its forest mass. Circular plots were installed with 12.62 m radius at an intensity of 5-6% ha-1. Through systematic unaligned sampling for lifting dasometric information and digging three pits stand-1, using stratified sampling for variables of the place. The application of statistical estimators, determined at an altitude of 2755 amsl, a total average tree volume estimated-1 of 2.32 m3 compared with 0.25 m3 at an altitude of 2929 amsl. Two areas of conservation and other forest use were defined. There are significant associations between total unit volume of T. blue against altitude, organic matter content, cationic exchange capacity at surface level and level of boron underlying level, generating a management strategy for each defined area.

  11. Stand mid-diameter extraction mid-distances influence in the harvesting costs of Eucalyptus globulus forest system in the Chile central zone

    International Nuclear Information System (INIS)

    Barrios, Alonso; Lopez, Ana M; Nieto, Victor M

    2008-01-01

    A whole tree and in-wood chipping harvesting system was studied by modelling and dynamic simulation. The iThink environment was used to build an application for simulating the operation of the harvesting system, using both deterministic and stochastic models (Isee systems, Inc. 2007). The variables used in this study were the mean diameter at breast height and the mean skidding distance of the stand. In this way, the influence of these variables in the systems cost per cubic meter was determined. This study exhibits a technical approach for establishing more appropriate payment fees, considering that the harvesting costs vary according to the stand characteristics.

  12. Effects of autohydrolysis of Eucalyptus urograndis and Eucalyptus grandis on influence of chemical components and crystallinity index.

    Science.gov (United States)

    da Silva Morais, Alaine Patrícia; Sansígolo, Cláudio Angeli; de Oliveira Neto, Mario

    2016-08-01

    Samples of Eucalyptus urograndis and Eucalyptus grandis sawdust were autohydrolyzed in aqueous conditions to reach temperatures in the range 110-190°C and reaction times of 0-150min in a minireactor. In each minireactor were used a liquor:wood ratio (10:1 L:kg dry wood), in order to assess the effects of the autohydrolysis severity and the crystalline properties of cellulose. The content of extractives, lignin, holocellulose, cellulose, hemicelluloses and crystallinity index obtained from the solid fraction after autohydrolysis of sawdust were determined. This study demonstrated that the hemicelluloses were extensively removed at 170 and 190°C, whereas cellulose was partly degraded to Eucalyptus urograndis and Eucalyptus grandis sawdust. The lignin content decreased, while the extractives content increased. It was defined that during autohydrolysis, had a slight decreased on crystalline structure of cellulose of Eucalyptus urogandis and Eucalyptus grandis. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Lectin cDNA and transgenic plants derived therefrom

    Science.gov (United States)

    Raikhel, Natasha V.

    2000-10-03

    Transgenic plants containing cDNA encoding Gramineae lectin are described. The plants preferably contain cDNA coding for barley lectin and store the lectin in the leaves. The transgenic plants, particularly the leaves exhibit insecticidal and fungicidal properties.

  14. Is Eucalyptus Cryptically Self-incompatible?

    Science.gov (United States)

    Horsley, Tasmien N; Johnson, Steven D

    2007-12-01

    The probability that seeds will be fertilized from self- versus cross-pollen depends strongly on whether plants have self-incompatibility systems, and how these systems influence the fate of pollen tubes. In this study of breeding systems in Eucalyptus urophylla and Eucalyptus grandis, epifluorescence microscopy was used to study pollen tube growth in styles following self- and cross-pollinations. Pollen tubes from self-pollen took significantly longer than those from cross-pollen to grow to the base of the style in both E. urophylla (120 h vs. 96 h) and E. grandis (96 h vs. 72 h). In addition, both species exhibited reduced seed yields following self-pollination compared with cross-pollination. The present observations suggest that, in addition to a late-acting self-incompatibility barrier, cryptic self-incompatibility could be a mechanism responsible for the preferential out-crossing system in these two eucalypt species.

  15. Development Potential, in a Greenhouse, of Cuttings of the Hybrid Eucalyptus grandis x Eucalyptus camaldulensis

    Directory of Open Access Journals (Sweden)

    M. N. Garcia

    2013-03-01

    Full Text Available Abstract: This study evaluated the potential for development in the greenhouse, cuttings of the hybrid of Eucalyptus grandis x Eucalyptus camaldulensis in Sinop - Mato Grosso and generated equations to estimate the percentage of rooting parameters. Were measured: the percentage of cuttings with some degree of modification, the percentage of cuttings in the percentage of cuttings with roots larger than 10 cm in length. Also measured was the neck diameter, height and leaf area index. The heights and leaf area showed lower development within the greenhouse. The rooting potential is based on curve speed of rooting and was found 12 days after staking. The identification of the optimal point of withdrawal of the shoots of the greenhouse through the intercept curves daily current increase curve (DCI e daily average increase (DAI, being 19 days after staking. The company derives the stakes with 24 days underdoing the potential of the species and structures used.Keywords: Eucalyptus, greenhouse management and rooting

  16. Water erosion risk prediction in eucalyptus plantations

    Directory of Open Access Journals (Sweden)

    Mayesse Aparecida da Silva

    2014-04-01

    Full Text Available Eucalyptus plantations are normally found in vulnerable ecosystems such as steep slope, soil with low natural fertility and lands that were degraded by agriculture. The objective of this study was to obtain Universal Soil Loss Equation (USLE factors and use them to estimate water erosion risk in regions with eucalyptus planted. The USLE factors were obtained in field plots under natural rainfall in the Rio Doce Basin, MG, Brazil, and the model applied to assess erosion risk using USLE in a Geographic Information System. The study area showed rainfall-runoff erosivity values from 10,721 to 10,642 MJ mm ha-1 h-1 yr-1. Some soils (Latosols had very low erodibility values (2.0 x 10-4 and 1.0 x 10-4t h MJ-1 mm-1, the topographic factor ranged from 0.03 to 10.57 and crop and management factor values obtained for native forest, eucalyptus and planted pasture were 0.09, 0.12 and 0.22, respectively. Water erosion risk estimates for current land use indicated that the areas where should receive more attention were mainly areas with greater topographic factors and those with Cambisols. Planning of forestry activities in this region should consider implementation of other conservation practices beyond those already used, reducing areas with a greater risk of soil erosion and increasing areas with very low risk.

  17. Estrogenicity and intersex in juvenile rainbow trout (Oncorhynchus mykiss) exposed to Pine/Eucalyptus pulp and paper production effluent in Chile

    International Nuclear Information System (INIS)

    Chiang, Gustavo; Barra, Ricardo; Díaz-Jaramillo, Mauricio; Rivas, Meyling; Bahamonde, Paulina; Munkittrick, Kelly R.

    2015-01-01

    Highlights: • Juvenile rainbow were exposed to Pine and Eucalyptus PPME along with an in situ bioassay downstream of the combined discharge. • Fish exposed to PPME showed induced levels of plasma vitellogenin and female gonad maturation. • Male fish showed intersex characteristics in laboratory and in situ assays. • Tertiary treated PPME from Eucalyptus production have stronger estrogenic effects on juvenile fish. - Abstract: Pulp and paper mill effluents (PPMEs) have been shown to increase gonad size, cause early maturation, and disrupt hormone functions in native and non-native Chilean fish. In this study, we assessed reproductive (plasma vitellogenin; VTG, gonad development) and metabolic (ethoxyresorufin-O-deethylase activity; EROD) end points, relative liver size (LSI) and condition factor (K) of juvenile female and male rainbow trout exposed to effluents. Unlike previous studies, which have focus either on the specific effects of effluent on fish in laboratory exposures or biotic population statuses downstream of discharge sites, we simultaneously assessed the impacts of PPMES on trout using two approaches: (1) laboratory exposures of tertiary treated PPME produced from processing Eucalyptus globulus or Pinus radiata; and (2) in situ bioassay downstream of the combined discharge of the same pulp mill. Despite an increase in the average gonadosomatic index (GSI) in exposed fish, no statistical differences in gonad size between exposed and unexposed individuals was detected. However, both female and male fish exposed to effluents showed significantly higher concentrations of plasma VTG, so more in fish exposed to Eucalyptus-based effluent when compared to Pinus PPME. In addition, male fish showed intersex characteristics in all exposure assays (Eucaliptus and Pinus) and, despite the low concentration of effluent in the river (<1% [v/v]), similar responses were observed in the caged fish. Finally, EROD activity was induced in both in situ exposures and

  18. Estrogenicity and intersex in juvenile rainbow trout (Oncorhynchus mykiss) exposed to Pine/Eucalyptus pulp and paper production effluent in Chile

    Energy Technology Data Exchange (ETDEWEB)

    Chiang, Gustavo, E-mail: gchiang@centromeri.cl [Canadian Rivers Institute and Department of Biology, University of New Brunswick, Saint John, NB E2L 4L5 (Canada); Barra, Ricardo [Aquatic Systems Research Unit, EULA–Chile Environmental Sciences Centre, University of Concepción, Concepcion (Chile); Díaz-Jaramillo, Mauricio [Laboratorio de Ecotoxicología y Contaminación Ambiental (ECoA), IIMyC-CONICET-UNMdP, Mar del Plata (Argentina); Rivas, Meyling [Department of Zoology, Faculty of Natural and Oceanographic Sciences, University of Concepcion, Concepcion (Chile); Bahamonde, Paulina; Munkittrick, Kelly R. [Canadian Rivers Institute and Department of Biology, University of New Brunswick, Saint John, NB E2L 4L5 (Canada)

    2015-07-15

    Highlights: • Juvenile rainbow were exposed to Pine and Eucalyptus PPME along with an in situ bioassay downstream of the combined discharge. • Fish exposed to PPME showed induced levels of plasma vitellogenin and female gonad maturation. • Male fish showed intersex characteristics in laboratory and in situ assays. • Tertiary treated PPME from Eucalyptus production have stronger estrogenic effects on juvenile fish. - Abstract: Pulp and paper mill effluents (PPMEs) have been shown to increase gonad size, cause early maturation, and disrupt hormone functions in native and non-native Chilean fish. In this study, we assessed reproductive (plasma vitellogenin; VTG, gonad development) and metabolic (ethoxyresorufin-O-deethylase activity; EROD) end points, relative liver size (LSI) and condition factor (K) of juvenile female and male rainbow trout exposed to effluents. Unlike previous studies, which have focus either on the specific effects of effluent on fish in laboratory exposures or biotic population statuses downstream of discharge sites, we simultaneously assessed the impacts of PPMES on trout using two approaches: (1) laboratory exposures of tertiary treated PPME produced from processing Eucalyptus globulus or Pinus radiata; and (2) in situ bioassay downstream of the combined discharge of the same pulp mill. Despite an increase in the average gonadosomatic index (GSI) in exposed fish, no statistical differences in gonad size between exposed and unexposed individuals was detected. However, both female and male fish exposed to effluents showed significantly higher concentrations of plasma VTG, so more in fish exposed to Eucalyptus-based effluent when compared to Pinus PPME. In addition, male fish showed intersex characteristics in all exposure assays (Eucaliptus and Pinus) and, despite the low concentration of effluent in the river (<1% [v/v]), similar responses were observed in the caged fish. Finally, EROD activity was induced in both in situ exposures and

  19. cDNA sequence quality data - Budding yeast cDNA sequencing project | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us Budding yeast cDNA sequencing project cDNA sequence quality data Data detail Data name cDNA sequence quality... data DOI 10.18908/lsdba.nbdc00838-003 Description of data contents Phred's quality score. P...tion Download License Update History of This Database Site Policy | Contact Us cDNA sequence quality

  20. allelopathic effects of eucalyptus tereticornis on phaseolus vulgaris

    African Journals Online (AJOL)

    user

    1. ALLELOPATHIC EFFECTS OF EUCALYPTUS TERETICORNIS ON PHASEOLUS. VULGARIS SEEDLINGS. Sale, F.A.. Department of Forestry and Wildlife, Faculty of ..... Sale, F.A. (2009). Allelopathic influence of Acacia auriculiformis. Eucalyptus citriodora and Gliricidia sepium on germination, growth and yield of millet.

  1. Genetic basis of resistance in eucalyptus spp. pathosystems

    Science.gov (United States)

    Acelino Couto Alfenas; Lucio Mauro da Silva Guimaraes; Marcos Deon Vilela Resende

    2012-01-01

    Eucalyptus is the most widely planted hardwood crop in world-wide tropical and subtropical regions because of its high growth rate, broad adaptability, and multipurpose wood properties. Until the 1970s, the Eucalyptus plantations in Brazil were practically disease free. However, plantations have continued to expand into warmer...

  2. Energy, chemicals and carbon: future options for the Eucalyptus ...

    African Journals Online (AJOL)

    This paper examines the current Eucalyptus value chain and the possible drivers that may impact upon it. The potential consequences of climate change on the growth and yield of Eucalyptus are discussed and the impact of the security of oil reserves and increased costs of fossil-fuel-derived energy and raw materials upon ...

  3. Diseases on Eucalyptus species in Zimbabwean plantations and ...

    African Journals Online (AJOL)

    Zimbabwe embarked on planting Eucalyptus species in the early 1900s. Based on a robust breeding programme, it has become a major source of seed for other countries in and outside Africa. Tree health surveys conducted on Eucalyptus in some east and southern African countries over the past two decades have ...

  4. Clonal differences in log end splitting in Eucalyptus grandis in ...

    African Journals Online (AJOL)

    This paper discusses the juvenile–mature correlation of log end splitting among Eucalyptus grandis clones from two trials and how differences in splitting relate to differences in wood density, pith-to-bark gradient and growth rate. Two approximately 20-year-old Eucalyptus grandis clonal trials at Bergvliet plantation were ...

  5. Eucalyptus Forest Information System for the Portuguese pulp and paper industry

    Science.gov (United States)

    Luis Fonseca; Rita Crespo; Henk Feith; Jose Luis Carvalho; Antonio Macedo; Joao Pedro Pina

    2000-01-01

    To support the management of the Portuguese eucalyptus forest, the Association of Portuguese Pulp and Paper Industries (CELPA) decided to develop a Eucalyptus Forest Information System (EFIS). The specific goals of the EFIS are: characterization and development of the eucalyptus forest over time; planning of successive national eucalyptus forest inventories; estimation...

  6. USE OF EUCALYPTUS WOOD FOR ORIENTED STRAND BOARD (OSB MANUFACTURING

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2004-06-01

    Full Text Available This research evaluated the potential use of Eucalyptus species for OSB manufacturing. The boards were manufactured at the density of 0,70 g/cm³ and 6% of the phenol-formaldheyde resin contents. The following Eucalyptus species were studied: E. grandis E. dunnii ,E. tereticornis E. saligna ,E. citriodora, and E. maculata. The results of the physical and mechanical property tests showed high potentiality of the uses of Eucalyptus grandis and Eucalyptus saligna for OSB manufacturing, Boards manufactured with Eucalyptus grandis wood presented similar or higher average values for physical and mechanical properties, in comparison to Pinus taeda, which is the main species used for OSB production in Brazil.

  7. Performance of Australian provenances of Eucalyptus grandis and Eucalyptus saligna in Hawaii

    Science.gov (United States)

    Roger G. Skolmen

    1986-01-01

    Australian provenances of Eucalyptus grandis and E. saligna were compared at four locations on the island of Hawaii to seek seed sources better than those in current use which were introduced earlier from unrecorded locations in Australia. A broad range of latitude and elevation was represented among the provenances. At all four...

  8. Variation in natural durability of seven Eucalyptus grandis x Eucalyptus urophylla hybrid clones

    Science.gov (United States)

    F.J.N. Franca; T.S.F.A. Franca; R.A Arango; B.M. Woodward; G.B. Vidaurre

    2017-01-01

    Programs aimed at developing clones of hybrid trees are commonly established in Brazil to meet the demands of various forest-based industries. These programs have continually improved the quality of eucalyptus wood, which has the potential to reduce deforestation by lowering demand for other high-value species. This is particularly true in the lumber market, but little...

  9. Oligo-carrageenan kappa increases NADPH, ascorbate and glutathione syntheses and TRR/TRX activities enhancing photosynthesis, basal metabolism, and growth in Eucalyptus trees

    Directory of Open Access Journals (Sweden)

    Alberto eGonzález

    2014-10-01

    Full Text Available In order to analyze the effect of OC kappa in redox status, photosynthesis, basal metabolism and growth in Eucalyptus globulus, trees were treated with water (control, with OC kappa at 1 mg mL-1, or treated with inhibitors of NAD(PH, ascorbate (ASC and glutathione (GSH syntheses and thioredoxin reductase (TRR activity, CHS-828, lycorine, buthionine sulfoximine (BSO and auranofin, respectively, and with OC kappa, and cultivated for 4 months. Treatment with OC kappa induced an increase in NADPH, ASC, and GSH syntheses, TRR and thioredoxin (TRX activities, photosynthesis, growth and activities of basal metabolism enzymes such as rubisco, glutamine synthetase (GlnS, adenosine 5´-phosphosulfate reductase (APR, involved in C, N and S assimilation, respectively, Krebs cycle and purine/pyrimidine synthesis enzymes. Treatment with inhibitors and OC kappa showed that increases in ASC, GSH and TRR/TRX enhanced NADPH synthesis, increases in NADPH and TRR/TRX enhanced ASC and GSH syntheses, and only the increase in NADPH enhanced TRR/TRX activities. In addition, the increase in NADPH, ASC, GSH and TRR/TRX enhanced photosynthesis and growth. Moreover, the increase in NADPH, ASC and TRR/TRX enhanced activities of rubisco, Krebs cycle and purine/pyrimidine synthesis enzymes, the increase in GSH, NADPH, and TRR/TRX enhanced APR activity, and the increase in NADPH and TRR/TRX enhanced GlnS activity. Thus, OC kappa increases NADPH, ASC and GSH syntheses leading to a more reducing redox status, the increase in NADPH, ASC, GSH syntheses and TRR/TRX activities are cross-talking events leading to activation of photosynthesis, basal metabolism and growth in Eucalyptus trees.

  10. Oligo-carrageenan kappa increases NADPH, ascorbate and glutathione syntheses and TRR/TRX activities enhancing photosynthesis, basal metabolism, and growth in Eucalyptus trees.

    Science.gov (United States)

    González, Alberto; Moenne, Fabiola; Gómez, Melissa; Sáez, Claudio A; Contreras, Rodrigo A; Moenne, Alejandra

    2014-01-01

    In order to analyze the effect of OC kappa in redox status, photosynthesis, basal metabolism and growth in Eucalyptus globulus, trees were treated with water (control), with OC kappa at 1 mg mL(-1), or treated with inhibitors of NAD(P)H, ascorbate (ASC), and glutathione (GSH) syntheses and thioredoxin reductase (TRR) activity, CHS-828, lycorine, buthionine sulfoximine (BSO), and auranofin, respectively, and with OC kappa, and cultivated for 4 months. Treatment with OC kappa induced an increase in NADPH, ASC, and GSH syntheses, TRR and thioredoxin (TRX) activities, photosynthesis, growth and activities of basal metabolism enzymes such as rubisco, glutamine synthetase (GlnS), adenosine 5'-phosphosulfate reductase (APR), involved in C, N, and S assimilation, respectively, Krebs cycle and purine/pyrimidine synthesis enzymes. Treatment with inhibitors and OC kappa showed that increases in ASC, GSH, and TRR/TRX enhanced NADPH synthesis, increases in NADPH and TRR/TRX enhanced ASC and GSH syntheses, and only the increase in NADPH enhanced TRR/TRX activities. In addition, the increase in NADPH, ASC, GSH, and TRR/TRX enhanced photosynthesis and growth. Moreover, the increase in NADPH, ASC and TRR/TRX enhanced activities of rubisco, Krebs cycle, and purine/pyrimidine synthesis enzymes, the increase in GSH, NADPH, and TRR/TRX enhanced APR activity, and the increase in NADPH and TRR/TRX enhanced GlnS activity. Thus, OC kappa increases NADPH, ASC, and GSH syntheses leading to a more reducing redox status, the increase in NADPH, ASC, GSH syntheses, and TRR/TRX activities are cross-talking events leading to activation of photosynthesis, basal metabolism, and growth in Eucalyptus trees.

  11. Radioactive cDNA microarray in neurospsychiatry

    International Nuclear Information System (INIS)

    Choe, Jae Gol; Shin, Kyung Ho; Lee, Min Soo; Kim, Meyoung Kon

    2003-01-01

    Microarray technology allows the simultaneous analysis of gene expression patterns of thousands of genes, in a systematic fashion, under a similar set of experimental conditions, thus making the data highly comparable. In some cases arrays are used simply as a primary screen leading to downstream molecular characterization of individual gene candidates. In other cases, the goal of expression profiling is to begin to identify complex regulatory networks underlying developmental processes and disease states. Microarrays were originally used with cell lines or other simple model systems. More recently, microarrays have been used in the analysis of more complex biological tissues including neural systems and the brain. The application of cDNA arrays in neuropsychiatry has lagged behind other fields for a number of reasons. These include a requirement for a large amount of input probe RNA in fluorescent-glass based array systems and the cellular complexity introduced by multicellular brain and neural tissues. An additional factor that impacts the general use of microarrays in neuropsychiatry is the lack of availability of sequenced clone sets from model systems. While human cDNA clones have been widely available, high quality rat, mouse, and drosophilae, among others are just becoming widely available. A final factor in the application of cDNA microarrays in neuropsychiatry is cost of commercial arrays. As academic microarray facilitates become more commonplace custom made arrays will become more widely available at a lower cost allowing more widespread applications. In summary, microarray technology is rapidly having an impact on many areas of biomedical research. Radioisotope-nylon based microarrays offer alternatives that may in some cases be more sensitive, flexible, inexpensive, and universal as compared to other array formats, such as fluorescent-glass arrays. In some situations of limited RNA or exotic species, radioactive membrane microarrays may be the most

  12. Radioactive cDNA microarray in neurospsychiatry

    Energy Technology Data Exchange (ETDEWEB)

    Choe, Jae Gol; Shin, Kyung Ho; Lee, Min Soo; Kim, Meyoung Kon [Korea University Medical School, Seoul (Korea, Republic of)

    2003-02-01

    Microarray technology allows the simultaneous analysis of gene expression patterns of thousands of genes, in a systematic fashion, under a similar set of experimental conditions, thus making the data highly comparable. In some cases arrays are used simply as a primary screen leading to downstream molecular characterization of individual gene candidates. In other cases, the goal of expression profiling is to begin to identify complex regulatory networks underlying developmental processes and disease states. Microarrays were originally used with cell lines or other simple model systems. More recently, microarrays have been used in the analysis of more complex biological tissues including neural systems and the brain. The application of cDNA arrays in neuropsychiatry has lagged behind other fields for a number of reasons. These include a requirement for a large amount of input probe RNA in fluorescent-glass based array systems and the cellular complexity introduced by multicellular brain and neural tissues. An additional factor that impacts the general use of microarrays in neuropsychiatry is the lack of availability of sequenced clone sets from model systems. While human cDNA clones have been widely available, high quality rat, mouse, and drosophilae, among others are just becoming widely available. A final factor in the application of cDNA microarrays in neuropsychiatry is cost of commercial arrays. As academic microarray facilitates become more commonplace custom made arrays will become more widely available at a lower cost allowing more widespread applications. In summary, microarray technology is rapidly having an impact on many areas of biomedical research. Radioisotope-nylon based microarrays offer alternatives that may in some cases be more sensitive, flexible, inexpensive, and universal as compared to other array formats, such as fluorescent-glass arrays. In some situations of limited RNA or exotic species, radioactive membrane microarrays may be the most

  13. Nutritional disorders in Eucalyptus citriodora. [In Portugese

    Energy Technology Data Exchange (ETDEWEB)

    Haag, H P; Simoes, J W; de Oliveira, G D; Sarruge, J R; Poggiani, F

    1977-01-01

    Eucalyptus spp., especially E. citriodora, when grown on the poor soils characteristic of cerrado areas in Brazil, sometimes show signs of an unusual disorder. The youngest leaves in the crown (and later most of the older leaves) wither but remain attached to the shoots, and the bark splits and exudes gum. Samples of leaves from a 4-year-old E. citriodora plantation in Sao Paulo that showed these symptoms were collected and analyzed for N, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn. The disorder is attributed to general malnutrition, and especially to a lack of S, Ca and P.

  14. Oldest Known Eucalyptus Macrofossils Are from South America

    Science.gov (United States)

    Zamaloa, María C.; Nixon, Kevin C.; González, Cynthia C.; Wilf, Peter; Cúneo, N. Rubén; Johnson, Kirk R.

    2011-01-01

    The evolutionary history of Eucalyptus and the eucalypts, the larger clade of seven genera including Eucalyptus that today have a natural distribution almost exclusively in Australasia, is poorly documented from the fossil record. Little physical evidence exists bearing on the ancient geographical distributions or morphologies of plants within the clade. Herein, we introduce fossil material of Eucalyptus from the early Eocene (ca. 51.9 Ma) Laguna del Hunco paleoflora of Chubut Province, Argentina; specimens include multiple leaves, infructescences, and dispersed capsules, several flower buds, and a single flower. Morphological similarities that relate the fossils to extant eucalypts include leaf shape, venation, and epidermal oil glands; infructescence structure; valvate capsulate fruits; and operculate flower buds. The presence of a staminophore scar on the fruits links them to Eucalyptus, and the presence of a transverse scar on the flower buds indicates a relationship to Eucalyptus subgenus Symphyomyrtus. Phylogenetic analyses of morphological data alone and combined with aligned sequence data from a prior study including 16 extant eucalypts, one outgroup, and a terminal representing the fossils indicate that the fossils are nested within Eucalyptus. These are the only illustrated Eucalyptus fossils that are definitively Eocene in age, and the only conclusively identified extant or fossil eucalypts naturally occurring outside of Australasia and adjacent Mindanao. Thus, these fossils indicate that the evolution of the eucalypt group is not constrained to a single region. Moreover, they strengthen the taxonomic connections between the Laguna del Hunco paleoflora and extant subtropical and tropical Australasia, one of the three major ecologic-geographic elements of the Laguna del Hunco paleoflora. The age and affinities of the fossils also indicate that Eucalyptus subgenus Symphyomyrtus is older than previously supposed. Paleoecological data indicate that the

  15. Oldest known Eucalyptus macrofossils are from South America.

    Directory of Open Access Journals (Sweden)

    María A Gandolfo

    Full Text Available The evolutionary history of Eucalyptus and the eucalypts, the larger clade of seven genera including Eucalyptus that today have a natural distribution almost exclusively in Australasia, is poorly documented from the fossil record. Little physical evidence exists bearing on the ancient geographical distributions or morphologies of plants within the clade. Herein, we introduce fossil material of Eucalyptus from the early Eocene (ca. 51.9 Ma Laguna del Hunco paleoflora of Chubut Province, Argentina; specimens include multiple leaves, infructescences, and dispersed capsules, several flower buds, and a single flower. Morphological similarities that relate the fossils to extant eucalypts include leaf shape, venation, and epidermal oil glands; infructescence structure; valvate capsulate fruits; and operculate flower buds. The presence of a staminophore scar on the fruits links them to Eucalyptus, and the presence of a transverse scar on the flower buds indicates a relationship to Eucalyptus subgenus Symphyomyrtus. Phylogenetic analyses of morphological data alone and combined with aligned sequence data from a prior study including 16 extant eucalypts, one outgroup, and a terminal representing the fossils indicate that the fossils are nested within Eucalyptus. These are the only illustrated Eucalyptus fossils that are definitively Eocene in age, and the only conclusively identified extant or fossil eucalypts naturally occurring outside of Australasia and adjacent Mindanao. Thus, these fossils indicate that the evolution of the eucalypt group is not constrained to a single region. Moreover, they strengthen the taxonomic connections between the Laguna del Hunco paleoflora and extant subtropical and tropical Australasia, one of the three major ecologic-geographic elements of the Laguna del Hunco paleoflora. The age and affinities of the fossils also indicate that Eucalyptus subgenus Symphyomyrtus is older than previously supposed. Paleoecological data

  16. BLEACHING EUCALYPTUS PULPS WITH SHORT SEQUENCES

    Directory of Open Access Journals (Sweden)

    Flaviana Reis Milagres

    2011-03-01

    Full Text Available Eucalyptus spp kraft pulp, due to its high content of hexenuronic acids, is quite easy to bleach. Therefore, investigations have been made attempting to decrease the number of stages in the bleaching process in order to minimize capital costs. This study focused on the evaluation of short ECF (Elemental Chlorine Free and TCF (Totally Chlorine Free sequences for bleaching oxygen delignified Eucalyptus spp kraft pulp to 90% ISO brightness: PMoDP (Molybdenum catalyzed acid peroxide, chlorine dioxide and hydrogen peroxide, PMoD/P (Molybdenum catalyzed acid peroxide, chlorine dioxide and hydrogen peroxide, without washing PMoD(PO (Molybdenum catalyzed acid peroxide, chlorine dioxide and pressurized peroxide, D(EPODP (chlorine dioxide, extraction oxidative with oxygen and peroxide, chlorine dioxide and hydrogen peroxide, PMoQ(PO (Molybdenum catalyzed acid peroxide, DTPA and pressurized peroxide, and XPMoQ(PO (Enzyme, molybdenum catalyzed acid peroxide, DTPA and pressurized peroxide. Uncommon pulp treatments, such as molybdenum catalyzed acid peroxide (PMo and xylanase (X bleaching stages, were used. Among the ECF alternatives, the two-stage PMoD/P sequence proved highly cost-effective without affecting pulp quality in relation to the traditional D(EPODP sequence and produced better quality effluent in relation to the reference. However, a four stage sequence, XPMoQ(PO, was required to achieve full brightness using the TCF technology. This sequence was highly cost-effective although it only produced pulp of acceptable quality.

  17. Eucalyptus and Water Use in South Africa

    Directory of Open Access Journals (Sweden)

    Janine M. Albaugh

    2013-01-01

    Full Text Available The Eucalyptus genus yields high rates of productivity and can be grown across a wide range of site types and climates for products such as pulp, fuelwood, or construction lumber. In addition, many eucalypts have the ability to coppice, making this genus an ideal candidate for use as a biofuel feedstock. However, the water use of Eucalyptus is a controversial issue, and the impacts of these fast-growing trees on water resources are well documented. Regardless, the demand for wood products and water continues to rise, providing a challenge to increase the productivity of forest plantations within water constraints. This is of particular relevance for water-limited countries such as South Africa which relies on exotic plantations to meet its timber needs. Research results from water use studies in South Africa are well documented and legislation restrictions limit further afforestation. This paper outlines techniques used to quantify the water use of eucalypt plantations and provides recommendations on where to focus future research efforts. Greater insights into the water use efficiency of clonal material are needed, as certain eucalypt clones show fast growth and low water use. To better understand water use efficiency, estimates should be combined with monitoring of stand canopy structure and measurements of physiological processes.

  18. Heterosis may result in selection favouring the products of long-distance pollen dispersal in Eucalyptus.

    Directory of Open Access Journals (Sweden)

    João Costa E Silva

    Full Text Available Using native trees from near the northern and southern extremities of the relatively continuous eastern distribution of Eucalyptus globulus in Tasmania, we compared the progenies derived from natural open-pollination (OP with those generated from within-region and long-distance outcrossing. Controlled outcrossing amongst eight parents - with four parents from each of the northern and southern regions - was undertaken using a diallel mating scheme. The progeny were planted in two field trials located within the species native range in southern Tasmania, and their survival and diameter growth were monitored over a 13-year-period. The survival and growth performances of all controlled cross types exceeded those of the OP progenies, consistent with inbreeding depression due to a combination of selfing and bi-parental inbreeding. The poorer survival of the northern regional (♀N♂N outcrosses compared with the local southern regional outcrosses (♀S♂S indicated differential selection against the former. Despite this mal-adaptation of the non-local ♀N♂N crosses at both southern sites, the survival of the inter-regional hybrids (♀N♂S and ♀S♂N was never significantly different from that of the local ♀S♂S crosses. Significant site-dependent heterosis was detected for the growth of the surviving long-distance hybrids. This was expressed as mid-parent heterosis, particularly at the more northern planting site. Heterosis increased with age, while the difference between the regional ♀N♂N and ♀S♂S crosses remained insignificant at any age at either site. Nevertheless, the results for growth suggest that the fitness of individuals derived from long-distance crossing may be better at the more northern of the planting sites. Our results demonstrate the potential for early-age assessments of pollen dispersal to underestimate realised gene flow, with local inbreeding under natural open-pollination resulting in selection favouring the

  19. Nanofibrillated cellulose (CNF) from eucalyptus sawdust as a dry strength agent of unrefined eucalyptus handsheets.

    Science.gov (United States)

    Vallejos, María Evangelina; Felissia, Fernando Esteban; Area, María Cristina; Ehman, Nanci Vanesa; Tarrés, Quim; Mutjé, Pere

    2016-03-30

    Nanofibrillated cellulose has been obtained from the cellulosic fraction of eucalyptus sawdust. The fractionation process involved the partial removal of hemicelluloses and lignin. CNF was obtained using TEMPO oxidation with NaOCl in basic medium followed by mechanical homogenization. The obtained CNF was subsequently used as a dry strength agent on unbleached unrefined eucalyptus pulp. The addition of 3, 6 and 9 wt.% of CNF increased lineally the tensile index of handsheets to about 55 N mg(-1) at 35°SR, compatible with papermachine runnability. The other mechanical properties also increased substantially, and porosity decreased moderately. The estimated specific surface and average diameter of these CNF were 60 m(2)g(-1), and of 41.0 nm, respectively. The addition of 9 wt.% of CNF produced an increase in mechanical strength, equivalent to that produced by PFI refining at 1600 revolutions. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Temporal dynamics of the response to Al stress in Eucalyptus grandis × Eucalyptus camaldulensis

    Directory of Open Access Journals (Sweden)

    Berenice K. de Alcântara

    2015-06-01

    Full Text Available Lipid peroxidation and root elongation of Eucalyptus grandis × Eucalyptus camaldulensis were studied under stress conditions in response to aluminum (Al, a metal known to limit agricultural productivity in acidic soils primarily due to reduced root elongation. In Brazil, the Grancam 1277 hybrid (E. grandis × E. camaldulensis has been planted in the "Cerrado", a region of the country with a wide occurrence of acidic soils. The present study demonstrated that the hybrid exhibited root growth reduction and increased levels of lipid peroxidation after 24h of treatment with 100 µM of Al, which was followed by a reduction in lipid peroxidation levels and the recovery of root elongation after 48h of Al exposure, suggesting a rapid response to the early stressful conditions induced by Al. The understanding of the temporal dynamics of Al tolerance may be useful for selecting more tolerant genotypes and for identifying genes of interest for applications in bioengineering.

  1. Eucalyptus grandis AND Eucalyptus dunnii USE FOR WOOD-CEMENT PANELS MANUFACTURING

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2008-03-01

    Full Text Available This research evaluated the potential use of Eucalyptus grandis and Eucalyptus dunnii wood for wood-cement panelsmanufacturing. The boards were manufactured at the density of 1,20 g/cm³, using portland cement as mineral bonding and woodfurnish without treatment, treated in cold water and hot water. The wood furnish of Pinus taeda was used as control. The resultsindicated that it is not necessary to treat E. grandis and E. dunni wood for wood-cement board manufacturing. In relation to woodspecies, the board manufactured with E. dunnii showed lower values of mechanical properties. However, boards manufactured of E.grandis wood showed satisfactory results in comparison to boards of P. taeda and the referenced values of BISON process and otherproducts cited in the pertnent literature, indicating the high potential for wood-cement board manufacture of this tree species.

  2. Properties of particleboards fabricated with eucalyptus (Eucalyptus urophylla, parica (Schizolobium amazonicum and vassoura (Sida spp. particles

    Directory of Open Access Journals (Sweden)

    Juliana Jerásio Bianche

    2012-12-01

    Full Text Available The genus Sida may become a promising alternative source for the production of particleboards. Nowadays it is used for manufacturing ropes due to the quality of its fibers, however, there is no research relating the production of particleboards using this lignocelluloses material. This study aimed to evaluate the effect of eucalyptus (Eucalyptus urophylla, paricá (Schizolobium amazonicum and vassoura (Sida spp. on the physical and mechanical properties of particleboard. Particleboard contained 25, 50, 75 and 100% of eucalyptus and paricá or eucalytus and vassoura or paricá an vassoura were fabricated. Two (6% and 8% amounts of urea-formaldehyde adhesive were used. The physical and mechanical properties were determined according to NBR / ABNT 14810-3 (ASSOCIAÇÃO BRASILEIRA DE NORMAS TÉCNICAS - ABNT, 2002. It was observed that increase in resin content in the improved some physical and mechanical properties. It was concluded that the amount of vassoura particles on panels had different effects depending on the species used. The increase in the percentage of vassoura particles in the Eucalyptus panels did not affect the internal bond, screw withdrawal and hardness. Increasing the percentage of vassoura particles on the panels produced with paricá did not affect its hardness and resistance to screw withdrawal. The panels produced with vassoura particle absorbed more water and, consequently, increased the thickness swelling, and therefore is not recommended for use in environments with high humidity. It was concluded that the genus Sida, in general, has potential for particleboard production.

  3. High-resolution genetic maps of Eucalyptus improve Eucalyptus grandis genome assembly.

    Science.gov (United States)

    Bartholomé, Jérôme; Mandrou, Eric; Mabiala, André; Jenkins, Jerry; Nabihoudine, Ibouniyamine; Klopp, Christophe; Schmutz, Jeremy; Plomion, Christophe; Gion, Jean-Marc

    2015-06-01

    Genetic maps are key tools in genetic research as they constitute the framework for many applications, such as quantitative trait locus analysis, and support the assembly of genome sequences. The resequencing of the two parents of a cross between Eucalyptus urophylla and Eucalyptus grandis was used to design a single nucleotide polymorphism (SNP) array of 6000 markers evenly distributed along the E. grandis genome. The genotyping of 1025 offspring enabled the construction of two high-resolution genetic maps containing 1832 and 1773 markers with an average marker interval of 0.45 and 0.5 cM for E. grandis and E. urophylla, respectively. The comparison between genetic maps and the reference genome highlighted 85% of collinear regions. A total of 43 noncollinear regions and 13 nonsynthetic regions were detected and corrected in the new genome assembly. This improved version contains 4943 scaffolds totalling 691.3 Mb of which 88.6% were captured by the 11 chromosomes. The mapping data were also used to investigate the effect of population size and number of markers on linkage mapping accuracy. This study provides the most reliable linkage maps for Eucalyptus and version 2.0 of the E. grandis genome. © 2014 CIRAD. New Phytologist © 2014 New Phytologist Trust.

  4. Development during indirect organogenesis in Eucalyptus grandis x ...

    African Journals Online (AJOL)

    BQWUPC1

    isolation or from a region of the stem immediately above the callus. Roots developed ... In this study, the same clone (Eucalyptus grandis urophylla hybrid) was used and .... strands and they linked these with shoot formation, whilst. Arvore et al.

  5. Eucalyptus microsatellites mined in silico: survey and evaluation

    Indian Academy of Sciences (India)

    2008-04-01

    Apr 1, 2008 ... Eucalyptus microsatellites mined in silico: survey and evaluation ... cific regions of the genome of different species (Marques et ..... Received 21 June 2007, in revised form 11 September 2007; accepted 12 September 2007.

  6. Antifungal activity of lemon, eucalyptus, thyme, oregano, sage and lavender essential oils against Aspergillus niger and Aspergillus tubingensis isolated from grapes

    Directory of Open Access Journals (Sweden)

    Miroslava Císarová

    2016-01-01

    Full Text Available Today, it is very important to find out the protection of products of natural origin as an alternative to synthetic fungicides. The promising alternative is the use of the essential oils (EOs. Essential oils from plants have great potential as a new source of fungicide to control the pathogenic fungi.The main objective of this study was evaluation of the antifungal activity of lemon (Citrus lemon L., eucalyptus (Eucalyptus globulus LABILL., thyme (Thymus vulgaris L., oregano (Origanum vulgare L. sage (Salvia officinalis L. and lavender (Lavandula angustifolia MILLER. EOs against Aspergillus niger and Aspergillus tubingensis isolated from grapes and their ability to affect the growth. It was tested by using the vapor contact with them. At first both tested isolates were identified by using PCR method. Sequence data of 18S rRNA supported the assignment of these isolates to the genus Aspergillus and species A. niger (ITS region: KT824061; RPB2: KT824060 and A. tubingensis (ITS region: KT824062; RPB2: KT824059. Second, EO antifungal activity was evaluated. The effect of the EO volatile phase was confirmed to inhibit growth of A. niger and A tubingensis. EOs were diluted in DMSO (dimethyl sulfoxide final volume of 100 μL. Only 50 μL this solution was distributed on a round sterile filter paper (1 x 1 cm by micropipette, and the paper was placed in the center of the lid of Petri dishes. Dishes were kept in an inverted position. The essential oils with the most significant activity were determined by method of graded concentration of oils - minimum inhibitory doses (MIDs. The most effective tested EOs were oregano and thyme oils, which totally inhibited growth of tested isolates for all days of incubation at 0.625 μL.cm-3 (in air with MFDs 0.125 μL.cm-3 (in air. Lavender EO was less active aginst tested strains (MIDs 0.313 μL.cm-3. The results showed that the tested EOs had antifungal activity, except lemon and eucalyptus. Sage EO was the only

  7. Methanol production from Eucalyptus wood chips. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Fishkind, H.H.

    1982-06-01

    This feasibility study includes all phases of methanol production from seedling to delivery of finished methanol. The study examines: production of 55 million, high quality, Eucalyptus seedlings through tissue culture; establishment of a Eucalyptus energy plantation on approximately 70,000 acres; engineering for a 100 million gallon-per-day methanol production facility; potential environmental impacts of the whole project; safety and health aspects of producing and using methanol; and development of site specific cost estimates.

  8. Discount Rate and Timber Rotation: the Case of Eucalyptus Saligna

    OpenAIRE

    Camilo Restrepo; Mauricio Alviar

    2010-01-01

    Eucalyptus is one of the most important economical and environmental species in Colombia. The main goal of this article is to study the relationship between the discount rate and the year of Eucalyptus Saligna optimal harvest, through the Fisher and Hotelling’s method. The methodology consists of calculating the maximum Net Present Value and performing a sensitivity analysis between the year of harvest and the discount rate. The area of study is located in the eastern region of the state of A...

  9. WEED CONTROL AND BORON NUTRITION ON Eucalyptus IN SILVOPASTORAL SYSTEM

    Directory of Open Access Journals (Sweden)

    Alexandre Magno Brighenti

    2015-04-01

    Full Text Available The objective of this study was to evaluate the control of weeds in the rows of eucalyptus (Eucalyptus urograndis with herbicides applied singly or combined with boron (B, as well as, the response of Eucalyptus plants to this micronutrient. The experiments were carried out in a split-plot with randomized complete block design, with three replicates. Six treatments were applied to the plots: (i weeded control, (ii control without weeding, (iii glyphosate (1080 g ae ha-1 + chlorimuron-ethyl (10 g ai ha-1 + 0.05% v / v mineral oil, (iv glyphosate (1080 g ae  ha-1 + isoxaflutole (112.5 g ai ha-1,  (v glyphosate (1080 g ae ha-1 and  (vi oxyfluorfen (480 g ai ha-1. The sub-plots consisted of the absence or presence of 4 kg of boric acid (H3BO3 - 17% B in 100 L of water. The addition of boric acid in the solution containing the herbicides did not affect the weed control. There was an increase in boron content in the soil and consequently an increase in the boron levels in the eucalyptus leaves. The combined application of herbicides plus boric acid is perfectly suitable for preventing dry of the pointer on eucalyptus plants. Keywords: Eucalyptus urograndis; chemical control; herbicides; micronutrients; Urochloa decumbens.

  10. cDNA structure, genomic organization and expression patterns of ...

    African Journals Online (AJOL)

    Visfatin was a newly identified adipocytokine, which was involved in various physiologic and pathologic processes of organisms. The cDNA structure, genomic organization and expression patterns of silver Prussian carp visfatin were described in this report. The silver Prussian carp visfatin cDNA cloned from the liver was ...

  11. Characterization of the porcine carboxypeptidase E cDNA

    DEFF Research Database (Denmark)

    Hreidarsdôttir, G.E.; Cirera, Susanna; Fredholm, Merete

    2007-01-01

    the sequence of the cDNA for the porcine CPE gene including all the coding region and the 3'-UTR region was generated. Comparisons with bovine, human, mouse, and rat CPE cDNA sequences showed that the coding regions of the gene are highly conserved both at the nucleotide and at the amino acid level. A very low...

  12. Infectious Maize rayado fino virus from cloned cDNA

    Science.gov (United States)

    Maize rayado fino virus (MRFV) is the type member of the marafiviruses within the family Tymoviridae. A cDNA clone from which infectious RNA can be transcribed was produced from a US isolate of MRFV (MRFV-US). Infectivity of transcripts derived from cDNA clones was demonstrated by infection of mai...

  13. cDNA encoding a polypeptide including a hevein sequence

    Energy Technology Data Exchange (ETDEWEB)

    Raikhel, N.V.; Broekaert, W.F.; Namhai Chua; Kush, A.

    1993-02-16

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1,018 nucleotides long and includes an open reading frame of 204 amino acids.

  14. ELASTIC CHARACTERIZATION OF Eucalyptus citriodora WOOD

    Directory of Open Access Journals (Sweden)

    Adriano Wagner Ballarin

    2003-01-01

    Full Text Available This paper contributed to the elastic characterization of Eucalyptus citriodora grown inBrazil, considering an orthotropic model and evaluating its most important elastic constants.Considering this as a reference work to establish basic elastic ratios — several important elasticconstants of Brazilian woods were not determined yet - the experimental set-up utilized one tree of 65years old from plantations of “Horto Florestal Navarro de Andrade”, at Rio Claro-SP, Brazil. All theexperimental procedures attended NBR 7190/97 – Brazilian Code for wooden structures –withconventional tension and compression tests. Results showed statistical identity between compressionand tension modulus of elasticity. The relation observed between longitudinal and radial modulus ofelasticity was 10 (EL/ER ≈ 10 and same relation, considering shear modulus (modulus of rigidity was20 (EL/GLR ≈ 20. These results, associated with Poisson’s ratios herein determined, allow theoreticalmodeling of wood mechanical behavior in structures.

  15. Molecular cloning of lupin leghemoglobin cDNA

    DEFF Research Database (Denmark)

    Konieczny, A; Jensen, E O; Marcker, K A

    1987-01-01

    Poly(A)+ RNA isolated from root nodules of yellow lupin (Lupinus luteus, var. Ventus) has been used as a template for the construction of a cDNA library. The ds cDNA was synthesized and inserted into the Hind III site of plasmid pBR 322 using synthetic Hind III linkers. Clones containing sequences...... specific for nodules were selected by differential colony hybridization using 32P-labeled cDNA synthesized either from nodule poly(A)+ RNA or from poly(A)+ RNA of uninfected root as probes. Among the recombinant plasmids, the cDNA gene for leghemoglobin was identified. The protein structure derived from...... its nucleotide sequence was consistent with known amino acid sequence of lupin Lb II. The cloned lupin Lb cDNA hybridized to poly(A)+ RNA from nodules only, which is in accordance with the general concept, that leghemoglobin is expressed exclusively in nodules. Udgivelsesdato: 1987-null...

  16. cDNA library Table - KAIKOcDNA | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available c00951-005 Description of data contents List of Bombyx mori cDNA libraries. Data file File name: kaiko_cdna_...library.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kaiko-cdna/LATEST/kaiko_cdna_library.zip File size:... 4.8 KB Simple search URL http://togodb.biosciencedbc.jp/togodb/view/kaiko_cdna_l

  17. QUALIDADE DA CELULOSE KRAFT-ANTRAQUINONA DE Eucalyptus dunnii PLANTADO EM CINCO ESPAÇAMENTOS EM RELAÇÃO AO Eucalyptus grandis E Eucalyptus saligna

    OpenAIRE

    Maria Cladis Mezzomo da Silva; Edvins Ratnieks; Teotônio Francisco de Assis; Celso Edmundo B. Foelkel; Jorge Vieira Gonzaga; Giovanni Willer Ferreira

    1997-01-01

    Este trabalho analisou o efeito de cinco espaçamentos de Eucalyptus dunnii (3 m x 1 m; 3 m x 1,5 m; 3 m x 2 m; 3 m x 3 m; 3 m x 4 m), para produção de celulose kraft-antraquinona, comparados com Eucalyptus grandis e Eucalyptus saligna (ambos no espaçamento 3 m x 2 m). Avaliaram-se a densidade básica e composição química da madeira original, analisando-se extrativos em diclorometano, pentosanas, lignina, solubilidade em NaOH8% e cinzas. Os cavacos foram submetidos a cozimento kraft-antraquinon...

  18. Plants of an Eucalyptus clone damaged by Scolytidae and Platypodidae (Coleoptera Plantas de Eucalyptus atacadas por Scolytidae e Platypodidae (Coleoptera

    Directory of Open Access Journals (Sweden)

    José Cola Zanuncio

    2005-05-01

    Full Text Available Species of Scolytidae and Platypodidae were evaluated in a plantation using a clone of Eucalyptus grandis x Eucalyptus urophylla in the State of Minas Gerais, Brazil. Wood disks with galleries were taken from 15 eucalyptus trees between 0.5 and 1.5 m height. After collection, all trees attacked were burned. Woodborers obtained from these disks were identified as Premnobius cavipennis, Premnobius ambitiosus, Dryocoetoides cristatus (Coleoptera: Scolytidae and Euplatypus parallelus (Coleoptera: Platypodidae. This is the first report of D. cristatus, a Brazilian native species, damaging eucalyptus, which shows its adaptation to this plant.O objetivo deste trabalho foi avaliar o ataque de Scolytidae e Platypodidae em talhão de clone Eucalyptus grandis x Eucalyptus urophylla na região de Montes Claros, Estado de Minas Gerais, Brasil. Discos de madeira com galerias desses insetos foram retirados de 15 árvores de eucalipto entre 0,5 e 1,5 m de altura. Após a coleta deste material, as árvores atacadas foram queimadas. Os indivíduos da ordem Coleoptera obtidos desses discos foram identificados como Premnobius cavipennis, Premnobius ambitiosus e Dryocoetoides cristatus (Coleoptera: Scolytidae e Euplatypus parallelus (Coleoptera: Platypodidae. O ataque de D. cristatus, espécie nativa do Brasil, ao eucalipto ainda não havia sido relatado, o que mostra sua adaptação a essa planta.

  19. Cloning of the human androgen receptor cDNA

    International Nuclear Information System (INIS)

    Govindan, M.V.; Burelle, M.; Cantin, C.; Kabrie, C.; Labrie, F.; Lachance, Y.; Leblanc, G.; Lefebvre, C.; Patel, P.; Simard, J.

    1988-01-01

    The authors discuss how in order to define the functional domains of the human androgen receptor, complementary DNA (cDNA) clones encoding the human androgen receptor (hAR) have been isolated from a human testis λgtll cDNA library using synthetic oligonnucleotide probes, homologous to segments of the human glucocorticoid, estradiol and progesterone receptors. The cDNA clones corresponding to the human glucocorticoid, estradiol and progesterone receptors were eliminated after cross-hybridization with their respective cDNA probes and/or after restriction mapping of the cDNA clones. The remaining cDNA clones were classified into different groups after analysis by restriction digestion and cross-hybridization. Two of the largest cDNA clones from each group were inserted into an expression vector in both orientations. The linearized plasmids were used as templates in in vitro transcription with T7 RNA polymerase. Subsequent in vitro translation of the purified transcripts in rabbit reticulocyte lysate followed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) permitted the characterization of the encoded polyeptides. The expressed proteins larger than 30,000 Da were analyzed for their ability to bind tritium-labelled dihydrotestosterone ([ 3 H] DHT) with high affinity and specificity

  20. Comparative transcriptomic analysis of the response to cold acclimation in Eucalyptus dunnii.

    Directory of Open Access Journals (Sweden)

    Yiqing Liu

    Full Text Available Eucalyptus dunnii is an important macrophanerophyte with high economic value. However, low temperature stress limits its productivity and distribution. To study the cold response mechanisms of E. dunnii, 5 cDNA libraries were constructed from mRNA extracted from leaves exposed to cold stress for varying lengths of time and were evaluated by RNA-Seq analysis. The assembly of the Illumina datasets was optimized using various assembly programs and parameters. The final optimized assembly generated 205,325 transcripts with an average length of 1,701 bp and N50 of 2,627 bp, representing 349.38 Mb of the E. dunnii transcriptome. Among these transcripts, 134,358 transcripts (65.4% were annotated in the Nr database. According to the differential analysis results, most transcripts were up-regulated as the cold stress prolonging, suggesting that these transcripts may be involved in the response to cold stress. In addition, the cold-relevant GO categories, such as 'response to stress' and 'translational initiation', were the markedly enriched GO terms. The assembly of the E. dunnii gene index and the GO classification performed in this study will serve as useful genomic resources for the genetic improvement of E. dunnii and also provide insights into the molecular mechanisms of cold acclimation in E. dunnii.

  1. 40 CFR 180.1241 - Eucalyptus oil; exemption from the requirement of a tolerance.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Eucalyptus oil; exemption from the... Exemptions From Tolerances § 180.1241 Eucalyptus oil; exemption from the requirement of a tolerance. Time-limited exemptions from the requirement of a tolerance are established for residues of eucalyptus oil on...

  2. 40 CFR 180.1271 - Eucalyptus oil; exemption from the requirement of a tolerance.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Eucalyptus oil; exemption from the... Exemptions From Tolerances § 180.1271 Eucalyptus oil; exemption from the requirement of a tolerance. An exemption from the requirement of tolerance is established for residues of eucalyptus oil in or on honey...

  3. [Growth effect of eucalyptus-acacia mixed plantation in South China].

    Science.gov (United States)

    Yang, Zeng-Jiang; Xu, Da-Ping; Chen, Wen-Ping; Huang, Lie-Jian; Li, Shang-Jun; Chen, Yuan

    2009-10-01

    Eucalyptus U6 and Acacia crassicarpa were mixed planted with different ratios and modes to investigate the growth parameters of the two tree species. In the 2-3 years old mixed plantation, the wind-throw of A. crassicarpa decreased markedly with increasing ratio of Eucalyptus U6, the decrement being 26.14% when the Eucalyptus U6/A. crassicarpa ratio was 3 : 1, but the survival rates of Eucalyptus U6 and A. crassicarpa had no significant difference under different planting modes. Mixed planting retarded the A. crassicarpa growth to some extent, with the DBH being 90% of that in pure A. crassicarpa stand. The mixed planting had little effects on the height growth of Eucalyptus U6, but promoted its DBH growth markedly, and the beneficial effect increased with increasing ratio of A. crassicarpa. In the 6 years old 1 : 1 Eucalyptus U6/A. crassicarpa plantation, the Eucalyptus U6 individuals with DBH > 15 cm occupied 32.1%; while in pure Eucalyptus U6 stand, they only accounted for 5.83%. Mixed planting with 2 : 1 Eucalyptus U6/A. crassicarpa could obtain a maximum total biomass of 198.8 m3 x hm(-2), which was 118.8% of the total biomass in pure Eucalyptus U6 stand, or 169.9% of that in pure A. crassicarpa stand. Mixture of Eucalyptus with Acacia would be a good choice to produce Eucalyptus trees with larger DBH.

  4. Sequence of human protamine 2 cDNA

    Energy Technology Data Exchange (ETDEWEB)

    Domenjoud, L; Fronia, C; Uhde, F; Engel, W [Universitaet Goettingen (West Germany)

    1988-08-11

    The authors report the cloning and sequencing of a cDNA clone for human protamine 2 (hp2), isolated from a human testis cDNA library cloned in the vector {lambda}-gt11. A 66mer oligonucleotide, that corresponds to an amino acid sequence which is highly conserved between hp2 and mouse protamine 2 (mp2) served as hybridization probe. The homology between the amino acid sequence deduced from our cDNA and the published amino acid sequence for hp2 is 100%.

  5. QUALIDADE DA CELULOSE KRAFT-ANTRAQUINONA DE Eucalyptus dunnii PLANTADO EM CINCO ESPAÇAMENTOS EM RELAÇÃO AO Eucalyptus grandis E Eucalyptus saligna

    Directory of Open Access Journals (Sweden)

    Maria Cladis Mezzomo da Silva

    1997-12-01

    Full Text Available Este trabalho analisou o efeito de cinco espaçamentos de Eucalyptus dunnii (3 m x 1 m; 3 m x 1,5 m; 3 m x 2 m; 3 m x 3 m; 3 m x 4 m, para produção de celulose kraft-antraquinona, comparados com Eucalyptus grandis e Eucalyptus saligna (ambos no espaçamento 3 m x 2 m. Avaliaram-se a densidade básica e composição química da madeira original, analisando-se extrativos em diclorometano, pentosanas, lignina, solubilidade em NaOH8% e cinzas. Os cavacos foram submetidos a cozimento kraft-antraquinona com álcali ativo variando de 18,5 a 21% para obter um número kappa 17± 1,5; sulfidez a 8%, antraquinona base madeira 0,05%, relação licor/madeira 4:1, tempo até temperatura máxima 60 minutos, tempo à temperatura máxima 45 minutos. A celulose marrom resultante do cozimento kraft-antraquinona foi caracterizada e analisada em seus aspectos físico-químicos (rendimentos, rejeitos, número kappa, viscosidade intrínseca, alvura e solubilidade em NaOH5%. Uma parte desta sofreu refinação em moinho Jökro ao nível de 35°SR e, juntamente com a polpa não-refinada, foram submetidas a testes físico-mecânicos e óticos (resistência à tração, alongamento, estouro, rasgo, volume específico, alvura, resistência ao ar Gurley e ascensão capilar Klemm. O maior consumo de álcali ativo (20,5% ocorreu em espaçamento 3 m x 4 m, proporcionando maior degradação da polpa. Todos os espaçamentos caracterizados apresentaram viabilidade para a produção de celulose kraft-antraquinona, podendo proporcionar redução nos custos de produção de celulose, devido aos menores consumos apresentados de álcali ativo e madeira quando comparados com Eucalyptus grandis e Eucalyptus saligna. Os valores de extrativos e cinzas mostraram-se elevados para Eucalyptus dunnii, potencializando possíveis impactos em termos de incrustações e fechamento de circuito no processo fabril. Diante dos resultados obtidos, a celulose de Eucalyptus dunnii atende às exig

  6. Method for construction of normalized cDNA libraries

    Science.gov (United States)

    Soares, Marcelo B.; Efstratiadis, Argiris

    1998-01-01

    This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.

  7. Cloning, sequencing and expression of cDNA encoding growth ...

    Indian Academy of Sciences (India)

    Unknown

    of medicine, animal husbandry, fish farming and animal ..... northern pike (Esox lucius) growth hormone; Mol. Mar. Biol. ... prolactin 1-luciferase fusion gene in African catfish and ... 1988 Cloning and sequencing of cDNA that encodes goat.

  8. Characterization of diazotrophic bacteria non-symbiotic associated with eucalyptus (eucalyptus sp.) in Codazzi, Cesar (Colombia)

    International Nuclear Information System (INIS)

    Obando Castellanos, Dolly Melissa; Burgos Zabala, Ludy Beatriz; Rivera Botia, Diego Mauricio; Rubiano Garrido, Maria Fernanda; Divan Baldini, Vera Lucia; Bonilla Buitrago, Ruth Rebeca

    2010-01-01

    The effect of climatic seasons (rainy and dry) and the stratum sample (rhizospheric soil, roots and leaves) the population of the genera Azotobacter, Beijerinckia, Derxia, Azospirillum, Herbaspirillum, Gluconacetobacter and Burkholderia in soil rhizosphere, roots and leaves of eucalyptus (eucalyptus sp.). It also assesses their ability to produce indoles compounds as plant growth promoters and their acetylene reduction activity as an indicator of biological fixation of nitrogen. The results showed no statistically significant differences in the Duncan test (p ≤ 0.05) in the population with respect to the climate epoch, suggesting that these bacteria are able to tolerate stress conditions by different physiological mechanisms. With respect to the stratum sample isolates attempts of Herbaspirillum sp. and Azospirillum sp. significant differences in rhizospheric soil and roots. we obtained 44 isolates of which were grouped by phenotypic characterization as 14 suspected of Beijerinckia sp., 12 Azotobacter sp., 8 Derxia sp., 4 Herbaspirillum sp., 5 Azospirillum sp., 1 Gluconacetobacter sp. and 1 Burkholderia sp. due to their high potential were selected isolates C27, C26 and C25. These four strains present the best values of efficiency in vitro, exceeding production values of the reference strains used (A. chroococcum (AC1) and a. brasilense (SP7)).

  9. RELATIONSHIP BETWEEN THE ANATOMY AND DRYING IN Eucalyptus grandis X Eucalyptus urophylla WOOD

    Directory of Open Access Journals (Sweden)

    Antônio José Vinha Zanuncio

    Full Text Available ABSTRACT Drying is an important step to using wood and anatomical characteristics influence this process. Thus, the objective of this work was to evaluate the relationship between anatomy and wood drying. Samples with 2 x 2 x 4 cm were obtained from eight Eucalyptus urophylla x Eucalyptus grandis clones at 1.3 m height to evaluate the anatomy and drying in climate chamber during 15 days. Cell wall fraction was established as the parameter that best correlated with drying, the Pearson correlation coefficient between this parameter and moisture was -0.8986, -0.6580, 0.9216, 0.8743, 0.7131 and 0.8727 for saturated wood, and after 1, 2.5, 5, 10 and 15 days of drying, respectively. The frequency and vessel size, as well as the height and width of the rays showed low relation with wood drying. Wood anatomy, mainly the cell wall fraction, influences moisture losses and should be considered in wood drying programs.

  10. Constructing and detecting a cDNA library for mites.

    Science.gov (United States)

    Hu, Li; Zhao, YaE; Cheng, Juan; Yang, YuanJun; Li, Chen; Lu, ZhaoHui

    2015-10-01

    RNA extraction and construction of complementary DNA (cDNA) library for mites have been quite challenging due to difficulties in acquiring tiny living mites and breaking their hard chitin. The present study is to explore a better method to construct cDNA library for mites that will lay the foundation on transcriptome and molecular pathogenesis research. We selected Psoroptes cuniculi as an experimental subject and took the following steps to construct and verify cDNA library. First, we combined liquid nitrogen grinding with TRIzol for total RNA extraction. Then, switching mechanism at 5' end of the RNA transcript (SMART) technique was used to construct full-length cDNA library. To evaluate the quality of cDNA library, the library titer and recombination rate were calculated. The reliability of cDNA library was detected by sequencing and analyzing positive clones and genes amplified by specific primers. The results showed that the RNA concentration was 836 ng/μl and the absorbance ratio at 260/280 nm was 1.82. The library titer was 5.31 × 10(5) plaque-forming unit (PFU)/ml and the recombination rate was 98.21%, indicating that the library was of good quality. In the 33 expressed sequence tags (ESTs) of P. cuniculi, two clones of 1656 and 1658 bp were almost identical with only three variable sites detected, which had an identity of 99.63% with that of Psoroptes ovis, indicating that the cDNA library was reliable. Further detection by specific primers demonstrated that the 553-bp Pso c II gene sequences of P. cuniculi had an identity of 98.56% with those of P. ovis, confirming that the cDNA library was not only reliable but also feasible.

  11. Should Exotic Eucalyptus be Planted in Subtropical China: Insights from Understory Plant Diversity in Two Contrasting Eucalyptus Chronosequences.

    Science.gov (United States)

    Wu, Jianping; Fan, Houbao; Liu, Wenfei; Huang, Guomin; Tang, Jianfu; Zeng, Ruijin; Huang, Jing; Liu, Zhanfeng

    2015-11-01

    Although Eucalyptus is widely planted in South China, whose effects on native biodiversity are unclear. The objective of this study was to quantify the richness and composition of understory plants in two contrasting Eucalyptus chronosequences in South China. One was in Zhangzhou City with plantation age of 2, 4, and 6 years after clear-cutting Chinese fir forests, while the other was in Heshan City with plantation age of 2, 3, and 24 years that reforested on barren lands. Results showed that the richness of understory plants and functional groups was not significantly altered in the Zhangzhou chronosequence, while increased in the 24-year-old plantations, with a significantly larger proportion of woody plants than the younger plantations for the Heshan chronosequence. Moreover, a higher richness of woody plants accompanied by a lower richness of herbaceous species was detected in the Zhangzhou chronosequence compared with the Heshan one. To balance the need for pulp production and plant diversity conservation, we suggest that intercropping approaches between exotic Eucalyptus plantations and native forests should be considered in the fast rotation Eucalyptus plantations. However, Eucalyptus plantations may be used as pioneer species to sustain ecosystem functioning for the degraded lands.

  12. Eucalyptus kraft pulp production: Thermogravimetry monitoring

    International Nuclear Information System (INIS)

    Barneto, Agustin G.; Vila, Carlos; Ariza, Jose

    2011-01-01

    Highlights: → Thermogravimetric analysis can be used to monitor the pulping process in a pulp mill. → ECF bleaching process affects the crystalline cellulose volatilization. → The fibre size has an influence on composition and thermal behavior of pulp. - Abstract: Under oxidative environment the thermal degradation of lignocellulosic materials like wood or pulp is sensitive to slight composition changes. For this, in order to complement the chemical and X-ray diffraction results, thermogravimetric analyses (TGA) were used to monitor pulp production in a modern pulp mill. Runs were carried out on crude, oxygen delignified and bleached pulps from three eucalyptus woods from different species and geographical origins. Moreover, with the modeling of thermogravimetric data, it was possible to obtain an approximate composition of samples which includes crystalline and amorphous cellulose. TGA results show that pulping has an intensive effect on bulk lignin and hemicellulose, but it has limited influence on the removal of these substances when they are linked to cellulose microfibril. The stages of oxygen delignification and bleaching, based in chlorine dioxide and hydrogen peroxide, increase the crystalline cellulose volatilization rate. These changes are compatible with a more crystalline microfibril. The influence of the fibre size on pulp composition, crystallinity and thermal degradation behavior was observed.

  13. Secagem de madeira serrada de Eucalyptus viminalis.

    Directory of Open Access Journals (Sweden)

    Emanuel C. E. Rozas M.

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 Neste estudo foi avaliado um programa de secagem para madeira de Eucalyptus viminalis de 25,0 mm de espessura, de duas árvores previamente aneladas (antes da derrubada e na confecção dos torretes e posteriormente vaporizadas. O programa de secagem constou de três diferentes fases de vaporização, sendo uma inicial de duas horas, para acelerar o processo de secagem, uma vaporização intermediária de quatro horas, para recuperação do colapso e uma vaporização final de uma hora, para diminuir o gradiente de umidade e as tensões de secagem, todas realizadas a 100ºC. Os bons resultados obtidos com o programa de secagem aplicado permitiu um aproveitamento de 72% da madeira (livre de defeitos. Estes resultados positivos podem ser atribuídos à aplicação de um programa de secagem gradual, às diferentes vaporizações a que a madeira foi submetida, como também ao fato de ter-se aplicado a técnica de anelamento e vaporização.

  14. Cloning of the cDNA for human 12-lipoxygenase

    International Nuclear Information System (INIS)

    Izumi, T.; Hoshiko, S.; Radmark, O.; Samuelsson, B.

    1990-01-01

    A full-length cDNA clone encoding 12-lipoxygenase was isolated from a human platelet cDNA library by using a cDNA for human reticulocyte 15-lipoxygenase as probe for the initial screening. The cDNA had an open reading frame encoding 662 amino acid residues with a calculated molecular weight of 75,590. Three independent clones revealed minor heterogeneities in their DNA sequences. Thus, in three positions of the deduced amino acid sequence, there is a choice between two different amino acids. The deduced sequence from the clone plT3 showed 65% identity with human reticulocyte 15-lipoxygenase and 42% identity with human leukocyte 5-lipoxygenase. The 12-lipoxygenase cDNA recognized a 3.0-kilobase mRNA species in platelets and human erythroleukemia cells (HEL cells). Phorbol 12-tetradecanoyl 13-acetate induced megakaryocytic differentiation of HEL cells and 12-lipoxygenase activity and increased mRNA for 12-lipoxygenase. The identity of the cloned 12-lipoxygenase was assured by expression in a mammalian cell line (COS cells). Human platelet 12-lipoxygenase has been difficult to purify to homogeneity. The cloning of this cDNA will increase the possibilities to elucidate the structure and function of this enzyme

  15. Estudo de algumas propriedades mecânicas da madeira de um híbrido clonal de Eucalyptus urophylla X Eucalyptus grandis Study of some mechanical properties of wood in a clonal hybrid of Eucalyptus urophylla X Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Fabrício Gomes Gonçalves

    2009-06-01

    Full Text Available A procura por madeiras oriundas de reflorestamentos destinadas à serraria é uma realidade já há muitos anos, principalmente aquelas das espécies do gênero Eucalyptus. Visando buscar novas informações importantes para esse mercado, este trabalho objetivou determinar algumas propriedades mecânicas da madeira de um híbrido clonal de Eucalyptus urophylla x Eucalyptus grandis de duas idades e provenientes de talhadia simples e de reforma. Os resultados indicaram que a madeira desse híbrido apresenta boas características tecnológicas, destacando-se a segunda tora (a partir de 3 m com as melhores propriedades de flexão estática (Módulo de Elasticidade - MOE e Módulo de Ruptura - MOR e Compressão Axial das fibras. As árvores de maior idade (166 meses e que sofreram dois desbastes apresentaram as melhores propriedades de flexão estática e compressão axial.The search for wood originated from reforestations destined to sawmill has been observed for many years, mainly for the Eucalyptus species. Searching for information to assist this market, the aim of this study was to determine some mechanical properties of the wood from a Eucalyptus urophylla x Eucalyptus grandis clonal hybrid, at two different ages and coming from coppice and reform. The results show that the wood of this clonal hybrid presents good technological characteristics and is the second best log (starting from 3,0 m, as to static bending properties (Module of Elasticity - MOE and Module of Rupture - MOR. The oldest trees (166 months - E2 which suffered two thinning presented the best results for the property of static bending and axial crushing (CA.

  16. Transcriptome and proteome analysis of Eucalyptus infected with Calonectria pseudoreteaudii.

    Science.gov (United States)

    Chen, Quanzhu; Guo, Wenshuo; Feng, Lizhen; Ye, Xiaozhen; Xie, Wanfeng; Huang, Xiuping; Liu, Jinyan

    2015-02-06

    Cylindrocladium leaf blight is one of the most severe diseases in Eucalyptus plantations and nurseries. There are Eucalyptus cultivars with resistance to the disease. However, little is known about the defense mechanism of resistant cultivars. Here, we investigated the transcriptome and proteome of Eucalyptus leaves (E. urophylla×E. tereticornis M1), infected or not with Calonectria pseudoreteaudii. A total of 8585 differentially expressed genes (|log2 ratio| ≥1, FDR ≤0.001) at 12 and 24hours post-inoculation were detected using RNA-seq. Transcriptional changes for five genes were further confirmed by qRT-PCR. A total of 3680 proteins at the two time points were identified using iTRAQ technique.The combined transcriptome and proteome analysis revealed that the shikimate/phenylpropanoid pathway, terpenoid biosynthesis, signalling pathway (jasmonic acid and sugar) were activated. The data also showed that some proteins (WRKY33 and PR proteins) which have been reported to involve in plant defense response were up-regulated. However, photosynthesis, nucleic acid metabolism and protein metabolism were impaired by the infection of C. pseudoreteaudii. This work will facilitate the identification of defense related genes and provide insights into Eucalyptus defense responses to Cylindrocladium leaf blight. In this study, a total of 130 proteins and genes involved in the shikimate/phenylpropanoid pathway, terpenoid biosynthesis, signalling pathway, cell transport, carbohydrate and energy metabolism, nucleic acid metabolism and protein metabolism in Eucalyptus leaves after infected with C. pseudoreteaudii were identified. This is the first report of a comprehensive transcriptomic and proteomic analysis of Eucalyptus in response to Calonectria sp. Copyright © 2014 Elsevier B.V. All rights reserved.

  17. Volume loss as a tool to assess kiln drying of eucalyptus wood

    Directory of Open Access Journals (Sweden)

    Djeison Cesar Batista

    Full Text Available In this study, we aimed to analyze the kiln drying quality of Eucalyptus grandis, Eucalyptus saligna and Eucalyptus dunnii woods with respect to volume loss. Wood from the three species was kiln dried together with the same drying schedule and conditions in a conventional-temperature pilot kiln. Three kinds of volume loss were evaluated: total - from saturated (initial to machined (final condition; shrinkage - from saturated to 10% moisture content; and machining - from 10% moisture content to machined condition. Eucalyptus grandis wood was the most dimensionally stable and presented the smallest volume loss due to shrinkage. Although they had different shrinkage behaviors, Eucalyptus grandis and Eucalyptus saligna woods presented the same drying quality regarding machining and total volume losses. These species can be considered the same for kiln drying. Eucalyptus dunnii wood presented the worst quality in drying, and should not be kiln dried in the same batch with the other species.

  18. Complex linkage between soil, soil water, atmosphere and Eucalyptus Plantations

    Science.gov (United States)

    Shukla, C.; Tiwari, K. N.

    2017-12-01

    Eucalyptus is most widely planted genus grown in waste land of eastern region of India to meet the pulp industry requirements. Sustainability of these plantations is of concern because in spite of higher demand water and nutrients of plantations, they are mostly planted on low-fertility soils. This study has been conducted to quantify effect of 25 years old, a fully established eucalyptus plantations on i.) Alteration in physico-chemical and hydrological properties of soil of eucalyptus plantation in comparison to soil of natural grassland and ii.) Spatio-temporal variation in soil moisture under eucalyptus plantations. Soil physico-chemical properties of two adjacent plots covered with eucatuptus and natural grasses were analyzed for three consecutive depths (i.e. 0-30 cm, 30-60 cm and 60-90 cm) with five replications in each plot. Soil infiltration rate and saturated hydraulic conductivity (Ks) were measured in-situ to incorporate the influence of macro porosity caused due to roots of plantations. Daily soil moisture at an interval of 10 cm upto 160 cm depth with 3 replications and Leaf Area Index (LAI) at an interval of 15 days with 5 replications were recorded over the year. Significant variations found at level of 0.05 between soil properties of eucalyptus and natural grass land confirm the effect of plantations on soil properties. Comparative results of soil properties show significant alteration in soil texture such as percent of sand, organic matter and Ks found more by 20%, 9% and 22% respectively in eucalyptus plot as compare to natural grass land. Available soil moisture (ASM) was found constantly minimum in top soil excluding rainy season indicate upward movement of water and nutrients during dry season. Seasonal variation in temperature (T), relative humidity (RH) and leaf area index (LAI) influenced the soil moisture extraction phenomenon. This study clearly stated the impact of long term establishment of eucalyptus plantations make considerable

  19. cDNA table - RPD | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available of data contents Results of homology search to cDNA clones in the KOME. Data file File name: rpd_cdna.zip F...ile URL: ftp://ftp.biosciencedbc.jp/archive/rpd/LATEST/rpd_cdna.zip File size: 15 KB Simple search URL http:...//togodb.biosciencedbc.jp/togodb/view/rpd_cdna#en Data acquisition method - Data

  20. Polyphenol compounds of the kino of Eucalyptus citriodora; Compostos polifenolicos do kino de Eucalyptus citriodora

    Energy Technology Data Exchange (ETDEWEB)

    Freitas, Marinalva Oliveira; Lima, Mary Anne S.; Silveira, Edilberto R. [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica]. E-mail: edil@ufc.br

    2007-07-01

    Phytochemical analysis of the kino of Eucalyptus citriodora led to the isolation of 1-O,2-O-digaloil-6-O-trans-p-cumaroil-beta-D-glucopyranoside, 1-O-trans-p-cumaroil-6-O-cinamoil-beta-D-glucopyranoside, alpha and beta 6-O-trans-p-cumaroil-D-glucopyranoside, 7-methylaromadendrin-4'-O-6{sup -}trans-p-cumaroil-beta-Dglucopyranoside, aromadendrin, aromadendrin-7-methyl-ether, naringenin, sakuranetin, kaempferol-7-methyl-ether and galic acid. Structural elucidation of the isolated compounds was established on the basis of spectral data, particularly by the use of 1D NMR and several 2D shift correlated NMR pulse sequences ({sup 1}H,{sup 1}H-COSY, HMQC, HMBC). (author)

  1. Second-strand cDNA synthesis: classical method

    International Nuclear Information System (INIS)

    Gubler, U.

    1987-01-01

    The classical scheme for the synthesis of double-stranded cDNA as it was reported in 1976 is described. Reverse transcription of mRNA with oligo(dT) as the primer generates first strands with a small loop at the 3' end of the cDNA (the end that corresponds to the 5' end of the mRNA). Subsequent removal of the mRNA by alkaline hydrolysis leaves single-stranded cDNA molecules again with a small 3' loop. This loop can be used by either reverse transcriptase or Klenow fragment of DNA polymerase I as a primer for second-strand synthesis. The resulting products are double-stranded cDNA molecules that are covalently closed at the end corresponding to the 5' end of the original mRNA. Subsequent cleavage of the short piece of single-stranded cDNA within the loop with the single-strand-specific S 1 nuclease generate open double-stranded molecules that can be used for molecular cloning in plasmids or in phage. Useful variations of this scheme have been described

  2. cDNA library construction of two human Demodexspecies.

    Science.gov (United States)

    Niu, DongLing; Wang, RuiLing; Zhao, YaE; Yang, Rui; Hu, Li; Lei, YuYang; Dan, WeiChao

    2017-06-01

    The research of Demodex, a type of pathogen causing various dermatoses in animals and human beings, is lacking at RNA level. This study aims at extracting RNA and constructing cDNA library for Demodex. First, P. cuniculiand D. farinaewere mixed to establish homogenization method for RNA extraction. Second, D. folliculorumand D. breviswere collected and preserved in Trizol, which were mixed with D. farinaerespectively to extract RNA. Finally, cDNA library was constructed and its quality was assessed. The results indicated that for D. folliculorum& D. farinae, the recombination rate of cDNA library was 90.67% and the library titer was 7.50 × 104 pfu/ml. 17 of the 59 positive clones were predicted to be of D. folliculorum; For D. brevis& D. farinae, the recombination rate was 90.96% and the library titer was 7.85 x104 pfu/ml. 40 of the 59 positive clones were predicted to be of D. brevis. Further detection by specific primers demonstrated that mtDNA cox1, cox3and ATP6 detected from cDNA libraries had 96.52%-99.73% identities with the corresponding sequences in GenBank. In conclusion, the cDNA libraries constructed for Demodexmixed with D. farinaewere successful and could satisfy the requirements for functional genes detection.

  3. Cloning and characterization of the human colipase cDNA

    International Nuclear Information System (INIS)

    Lowe, M.E.; Rosenblum, J.L.; McEwen, P.; Strauss, A.W.

    1990-01-01

    Pancreatic lipase hydrolyzes dietary triglycerides to monoglycerides and fatty acids. In the presence of bile salts, the activity of pancreatic lipase is markedly decreased. The activity can be restored by the addition of colipase, a low molecular weight protein secreted by the pancreas. The action of pancreatic lipase in the gut lumen is dependent upon its interaction with colipase. As a first step in elucidating the molecular events governing the interaction of lipase and colipase with each other and with fatty acids, a cDNA encoding human colipase was isolated from a λgt11 cDNA library with a rabbit polyclonal anti-human colipase antibody. The full-length 525 bp cDNA contained an open reading frame encoding 112 amino acids, including a 17 amino acid signal peptide. The predicted sequence contains 100% of the published protein sequence for human colipase determined by chemical methods, but predicts the presence of five additional NH 2 -terminal amino acids and four additional COOH-terminal amino acids. Comparison of the predicted protein sequence with the known sequences of colipase from other species reveals regions of extensive identity. The authors report, for the first time, a cDNA for colipase. The cDNA predicts a human procolipase an suggests that there may also be processing at the COOH-terminus. The regions of identity with colipase from other species will aid in defining the interaction with lipase and lipids through site-specific mutagenesis

  4. Statistical sampling and modelling for cork oak and eucalyptus stands

    NARCIS (Netherlands)

    Paulo, M.J.

    2002-01-01

    This thesis focuses on the use of modern statistical methods to solve problems on sampling, optimal cutting time and agricultural modelling in Portuguese cork oak and eucalyptus stands. The results are contained in five chapters that have been submitted for publication

  5. Eucalyptus: an open-source cloud computing infrastructure

    Energy Technology Data Exchange (ETDEWEB)

    Nurmi, Daniel; Wolski, Rich; Grzegorczyk, Chris; Obertelli, Graziano; Soman, Sunil; Youseff, Lamia; Zagorodnov, Dmitrii, E-mail: rich@cs.ucsb.ed [Computer Science Department, University of California, Santa Barbara, CA 93106 (United States) and Eucalyptus Systems Inc., 130 Castilian Dr., Goleta, CA 93117 (United States)

    2009-07-01

    Utility computing, elastic computing, and cloud computing are all terms that refer to the concept of dynamically provisioning processing time and storage space from a ubiquitous 'cloud' of computational resources. Such systems allow users to acquire and release the resources on demand and provide ready access to data from processing elements, while relegating the physical location and exact parameters of the resources. Over the past few years, such systems have become increasingly popular, but nearly all current cloud computing offerings are either proprietary or depend upon software infrastructure that is invisible to the research community. In this work, we present Eucalyptus, an open-source software implementation of cloud computing that utilizes compute resources that are typically available to researchers, such as clusters and workstation farms. In order to foster community research exploration of cloud computing systems, the design of Eucalyptus emphasizes modularity, allowing researchers to experiment with their own security, scalability, scheduling, and interface implementations. In this paper, we outline the design of Eucalyptus, describe our own implementations of the modular system components, and provide results from experiments that measure performance and scalability of a Eucalyptus installation currently deployed for public use. The main contribution of our work is the presentation of the first research-oriented open-source cloud computing system focused on enabling methodical investigations into the programming, administration, and deployment of systems exploring this novel distributed computing model.

  6. Eucalyptus: an open-source cloud computing infrastructure

    International Nuclear Information System (INIS)

    Nurmi, Daniel; Wolski, Rich; Grzegorczyk, Chris; Obertelli, Graziano; Soman, Sunil; Youseff, Lamia; Zagorodnov, Dmitrii

    2009-01-01

    Utility computing, elastic computing, and cloud computing are all terms that refer to the concept of dynamically provisioning processing time and storage space from a ubiquitous 'cloud' of computational resources. Such systems allow users to acquire and release the resources on demand and provide ready access to data from processing elements, while relegating the physical location and exact parameters of the resources. Over the past few years, such systems have become increasingly popular, but nearly all current cloud computing offerings are either proprietary or depend upon software infrastructure that is invisible to the research community. In this work, we present Eucalyptus, an open-source software implementation of cloud computing that utilizes compute resources that are typically available to researchers, such as clusters and workstation farms. In order to foster community research exploration of cloud computing systems, the design of Eucalyptus emphasizes modularity, allowing researchers to experiment with their own security, scalability, scheduling, and interface implementations. In this paper, we outline the design of Eucalyptus, describe our own implementations of the modular system components, and provide results from experiments that measure performance and scalability of a Eucalyptus installation currently deployed for public use. The main contribution of our work is the presentation of the first research-oriented open-source cloud computing system focused on enabling methodical investigations into the programming, administration, and deployment of systems exploring this novel distributed computing model.

  7. Mixed plantations of Eucalyptus and leguminous trees enhance biomass production

    Science.gov (United States)

    Dean S. DeBell; Craig D. Whitesell; Thomas H. Schubert

    1985-01-01

    Two Eucalyptus species-E. saligna Sm. and E. grandis Hill-are especially favored in Hawaii forwood, fiber, and fuel production because of their quick growth and high yields. Their growth is limited, however, on many sites by low levels of available nitrogen. Supplemental nitrogen can be provided by nitrogen-...

  8. Review Paper Les plantations d'Eucalyptus au Sahel : distribution ...

    African Journals Online (AJOL)

    SOUMARE

    la Francophonie pour avoir soutenu cette étude à travers une bourse de formation. RESUME ..... 1968-2000. Climat saharien. Climat sahélien. Climat soudano-sahélien. Frontières des .... universalis: Global cultivated Eucalyptus forests Map ...

  9. Productivity gains by fertilisation in Eucalyptus urophylla clonal ...

    African Journals Online (AJOL)

    Productivity gains by fertilisation in Eucalyptus urophylla clonal plantations across gradients in site and stand conditions. ... The control plot may typically be a permanent plot of an inventory network, providing representative information for a company's decisionmaking. The paired twin-plot receives intensive management ...

  10. Genetic diversity among four Eucalyptus species (myrtaceae) based ...

    African Journals Online (AJOL)

    The results indicate that 16 positive and 13 negative markers were detected. The marker fragments size ranged between 175 to 630 bp for the negative markers and 235 to 945 bp for the positive markers. Key words: Eucalyptus, random amplified polymorphic DNA (RAPD), genetic diversity, DNA fingerprinting PCR, ...

  11. Metabolic responses of Eucalyptus species to different temperature regimes

    NARCIS (Netherlands)

    Mokochinski, Joao Benhur; Mazzafera, Paulo; Sawaya, Alexandra Christine Helena Frankland; Mumm, Roland; Vos, de Ric Cornelis Hendricus; Hall, Robert David

    2018-01-01

    Species and hybrids of Eucalyptus are the world's most widely planted hardwood trees. They are cultivated across a wide range of latitudes and therefore environmental conditions. In this context, comprehensive metabolomics approaches have been used to assess how different temperature regimes may

  12. GENERAL EQUATIONS OF CARBONIZATION OF EUCALYPTUS SPP KINETIC MECHANISMS

    Directory of Open Access Journals (Sweden)

    Túlio Jardim Raad

    2006-06-01

    Full Text Available In the present work, a set of general equations related to kinetic mechanism of wood compound carbonization: hemicelluloses, cellulose and lignin was obtained by Avrami-Eroffev and Arrhenius equations and Thermogravimetry of Eucalyptus cloeziana, Eucalyptus camaldulensis, Corymbia citriodora, Eucalyptus urophylla and Eucalyptus grandis samples, TG-Isothermal and TG-Dynamic. The different thermal stabilities and decomposition temperature bands of those species compounds were applied as strategy to obtain the kinetic parameters: activation energy, exponential factor and reaction order. The kinetic model developed was validated by thermogravimetric curves from carbonization of others biomass such as coconut. The kinetic parameters found were - Hemicelluloses: E=98,6 kJmol, A=3,5x106s-1 n=1,0; - Cellulose: E=182,2 kJmol, A=1,2x1013s-1 n=1,5; - Lignin: E=46,6 kJmol, A=2,01s-1 n=0,41. The set of equations can be implemented in a mathematical model of wood carbonization simulation (with heat and mass transfer equations with the aim of optimizing the control and charcoal process used to produce pig iron.

  13. Soil fertility and growth of Eucalyptus grandis in Brazil under ...

    African Journals Online (AJOL)

    Silvicultural operations such as soil preparation, logging residue management and application of fertilisers can influence soil fertility, and hence nutrient uptake and tree growth. This paper reports the effect of site management practices of minimum and intensive cultivation of the soil on the growth of a stand of Eucalyptus ...

  14. Ongoing molecular studies of Eucalyptus powdery mildew in Brazil

    Science.gov (United States)

    N. R. Fonseca; L. M. S. Guimaraes; R. P. Pires; Ned Klopfenstein; M. -S. Kim; A. C. Alfenas

    2016-01-01

    Powdery mildew diseases are caused by biotrophic fungi in the Erysiphales. These fungal pathogens are easily observed by the whitish powdery appearance caused by their colonization of the aerial surfaces on living plants (Stadnik & Rivera, 2001) (Figure 1). In Brazil, powdery mildew of Eucalyptus spp is increasing under the current nursery production...

  15. Physiological responses to glyphosate are dependent on Eucalyptus urograndis genotype

    Science.gov (United States)

    Two experiments were conducted to evaluate the response of Eucalyptus urograndis genotypes (C219 and GG100) to glyphosate in growth chambers. As glyphosate dose increased (18 up to 720 g ae ha-1), CO2 assimilation rate, transpiration rate, and stomatal conductance decreased fastest and strongest in ...

  16. A tool for identifying potential Eucalyptus nitens seed orchard sites ...

    African Journals Online (AJOL)

    Shy seed production in orchards of Eucalyptus nitens is a major barrier to the deployment of genetic gain in South African plantations. A machine learning method was used to identify optimal sites for the establishment of E. nitens seed orchards within the plantation forestry landscape of the summer rainfall region of South ...

  17. Using chlorophyll fluorescence to determine stress in Eucalyptus ...

    African Journals Online (AJOL)

    Using chlorophyll fluorescence to determine stress in Eucalyptus grandis seedlings: scientific paper. ... Southern Forests: a Journal of Forest Science ... factors affect the functioning of the photosynthetic system, the status of the photosynthetic apparatus is a good indicator of the plant in terms of stress and stress adaptation.

  18. Stomatal characteristics of Eucalyptus grandis clonal hybrids in ...

    African Journals Online (AJOL)

    This study describes the stomatal response occurring during water stress and subsequent recovery of three Eucalyptus grandis clonal hybrids. The aim was to investigate the degree to which stomatal conductance (gs) and stomatal density differ between the clonal hybrids across seasons and in response to water stress.

  19. Diagnosing foliar nutrient dynamics of Eucalyptus grandis in ...

    African Journals Online (AJOL)

    Fertilisation is one of the most cost-effective methods of increasing and maintaining the productivity of Eucalyptus grandis plantations in South Africa. This silvicultural practice can be optimised by using the foliar nutrient ratios measured in plants at maximum growth as a guideline for fertiliser application. The foliar nutrient ...

  20. Shoot and root morphogenesis from Eucalyptus grandis x urophylla ...

    African Journals Online (AJOL)

    Eucalyptus grandis x urophylla plantlets were regenerated via indirect organogenesis. Histological assessment of their development focused on identifying the calli, the differentiation of shoots from the calli and the shoot-root junction from the nascent shoots. Vascular tissue formation within the callus preceded that of ...

  1. Impact of Eucalyptus plantations on the avian breeding community ...

    African Journals Online (AJOL)

    The nesting bird species in natural forests and Eucalyptus plantations on the Amani Plateau, East Usambara, were studied during the breeding season of September 2003 to March 2004. Some forest birds — like barbets, batis, broadbills, doves, flycatchers, greenbuls, hornbills, and tinkerbirds — utilised similar nest sites ...

  2. Eucalyptus plantlet growth in relation to foliar application with ...

    African Journals Online (AJOL)

    In one experiment at greenhouse, condition was established to determine the effects of complete fertilizers on growth of plantlet Eucalyptus camaldulensis in Southeast of Iran. This experiment was conducted in the Agricultural Research Center, University of Zabol, in autumn year of 2008. This study was designed as a ...

  3. Responses of Eucalyptus species to fertilizer applications made at ...

    African Journals Online (AJOL)

    Early research trials in South Africa have shown that Eucalyptus species generally respond positively to the addition of nutrients at planting. However, as most of these research trials were located in KwaZulu-Natal, it was important to investigate the nature of the response in other afforested regions of the country where this ...

  4. Soil carbon estimation from eucalyptus grandis using canopy spectra

    African Journals Online (AJOL)

    Mapping soil fertility parameters, such as soil carbon (C), is fundamentally important for forest management and research related to forest growth and climate change. This study seeks to establish the link between Eucalyptus grandis canopy spectra and soil carbon using raw and continuum-removed spectra. Canopy-level ...

  5. Growth and yield models for Eucalyptus grandis grown in Swaziland ...

    African Journals Online (AJOL)

    The aim of this study was to develop a stand-level growth and yield model for short-rotationEucalyptus grandis grown for pulp wood production at Piggs Peak in Swaziland. The data were derived from a Nelder 1a spacing trial established with E. grandis clonal cuttings in 1998 and terminated in 2005. Planting density ...

  6. The conservation and breeding of Eucalyptus urophylla : a case ...

    African Journals Online (AJOL)

    Eucalyptus urophylla is one of the most commercially important forest species in the world, primarily as a hybrid parent. However, the conservation status of the majority of the populations where it naturally occurs on seven islands in eastern Indonesia range from critically endangered to vulnerable. We examine the ...

  7. assessment of acidity levels in eucalyptus camaldulensis barks from ...

    African Journals Online (AJOL)

    BARTH EKWUEME

    Bark samples of Eucalyptus camaldulensis obtained from Bauchi and Gombe States were analysed spectrophotometrically for their sulphate-sulphur content. The aim was to assess the extent of sulphur pollution in the environment. The results showed that S concentration ranged from 0.79 to 1.70mg/g for samples from ...

  8. Sub-Acute Hepatoxicity of Aqueous Leaf Extract of Eucalyptus ...

    African Journals Online (AJOL)

    Sub–acute toxicity study of the aqueous leaf extract of Eucalyptus camaldulensis was carried out on albino rats. Doses of 250mg, 500mg, 750mg and 1000mg per kilogram body weight of the extract were administered orally for 21 days. The activities of Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), ...

  9. Assessment of acidity levels in Eucalyptus Camaldulensis barks ...

    African Journals Online (AJOL)

    Bark samples of Eucalyptus camaldulensis obtained from Bauchi and Gombe States were analysed spectrophotometrically for their sulphate-sulphur content. The aim was to assess the extent of sulphur pollution in the environment. The results showed that S concentration ranged from 0.79 to 1.70mg/g for samples from ...

  10. Coppicing potential of Eucalyptus nitens : results from a field survey ...

    African Journals Online (AJOL)

    In order to determine factors which could have a positive influence on the coppicing potential of Eucalyptus nitens , a field survey was carried out at Draycott, near Estcourt in the KwaZulu-Natal Midlands. Five measures of the ability to coppice (stump survival, height of coppice, number of dominant shoots, coppicing ...

  11. Anti-inflammatory of both Eucalyptus spp. and Pistascia lentiscus ...

    African Journals Online (AJOL)

    Background: Eucalyptus spp. and Pistascia lentiscus are among the Palestinian trees that are traditionally used in folkloric medicine in treating many diseases; leaves of which are thought to have anti-inflammatory, antibacterial and antioxidant effects. The goal of this study is to evaluate the in vitro inhibitory effect of ...

  12. Genetic improvement of Eucalyptus grandis using breeding seedling ...

    African Journals Online (AJOL)

    Eucalyptus grandis is commercially important in Zimbabwe and a breeding program has been in progress since 1962. A classical breeding strategy was used initially but, in 1981, the Multiple Population Breeding Strategy (MPBS) was implemented and the concept of the Breeding Seedling Orchard (BSO) became central to ...

  13. Growth models for six Eucalyptus species in Angola | Delgado ...

    African Journals Online (AJOL)

    This study developed growth models for Eucalyptus saligna Sm., E. camaldulensis Dehnh., E. macarthurii H.Deane & Maiden, E. resinifera Sm., E. siderophloia Benth. and E. grandis Hill ex. Maiden, for the central highlands of Angola, and used these models to simulate the development of stand characteristics.

  14. Assessment of wood density of seven clones of Eucalyptus grandis ...

    African Journals Online (AJOL)

    With the objective of evaluating the correlation of wood basic density with age in seven Eucalyptus grandis clones planted in Brazil, five trees in each clone were sampled at the ages of 0, 5, 1, 5, 2, 5, 3, 5, 4, 5 and 7, 5 years. The analysis of these samples showed that the intraclonal variation of the basic density (except for 0, ...

  15. Developing clones of Eucalyptus cloeziana resistant to rust (Puccinia psidii)

    Science.gov (United States)

    Rafael F. Alfenas; Marcelo M. Coutinho; Camila S. Freitas; Rodrigo G. Freitas; Acelino C. Alfenas

    2012-01-01

    Besides its high resistance to Chrysoporthe cubensis canker, Eucalyptus cloeziana F. Muell. is a highly valuable tree species for wood production. It can be used for furniture, electric poles, fence posts, and charcoal. Nevertheless, it is highly susceptible to the rust caused by Puccinia psidii, which...

  16. Nutritional sustainability of Eucalyptus plantations : a case study at ...

    African Journals Online (AJOL)

    The nutritional sustainability of a short-rotation Eucalyptus grandis plantation system was evaluated in a trial located at Karkloof, KwaZulu-Natal, South Africa, by determining nutrient pools and fluxes. Nutrient pools in the forest floor and biomass (above- and below-ground) were assessed by destructive sampling. The size ...

  17. Growth response at age 10 years of five Eucalyptus genotypes ...

    African Journals Online (AJOL)

    Thus, following the 1999 drought event a trial was established in a drought-prone area, planted to a number of genotypes that were deemed to be more tolerant to water stress than Eucalyptus grandis. Each species was planted at stand densities of 816, 1 600 and 2 066 stems ha-1, where 1 600 stems ha-1 represented the ...

  18. Estimating foliar nitrogen in Eucalyptus using vegetation indexes

    Directory of Open Access Journals (Sweden)

    Luiz Felipe Ramalho de Oliveira

    Full Text Available ABSTRACT Nitrogen (N has commonly been applied in Eucalyptus stands in Brazil and it has a direct relation with biomass production and chlorophyll content. Foliar N concentrations are used to diagnose soil and plant fertility levels and to develop N fertilizer application rates. Normally, foliar N is obtained using destructive methods, but indirect analyses using Vegetation Indexes (VIs may be possible. The aim of this work was to evaluate VIs to estimate foliar N concentration in three Eucalyptus clones. Lower crown leaves of three clonal Eucalyptus plantations (25 months old were classified into five color patterns using the Munsell Plant Tissue Color Chart. For each color, N concentration was determined by the Kjeldahl method and foliar reflectance was measured using a CI-710 Miniature Leaf Spectrometer. Foliar reflectance data were used to obtain the VIs and the VIs were used to estimate N concentrations. In the visible region, the relationship between N concentration and reflectance percentage was negative. The highest correlations between VIs and N concentrations were obtained by the Inflection Point Position (IPP, r = 0.97, Normalized Difference Red-Edge (reNDVI, r = 0.97 and Modified Red-Edge Normalized Difference Vegetation Index (mNDI, r = 0.97. Vegetation indexes on the red edge region provided the most accurate estimates of foliar N concentration. The reNDVI index provided the best N concentration estimates in leaves of different colors of Eucalyptus urophylla × grandis and Eucalyptus urophylla × urophylla (R2 = 0.97 and RMSE = 0.91 g kg−1.

  19. Thermal characterization of oil palm fiber and eucalyptus in torrefaction

    International Nuclear Information System (INIS)

    Chen, Wei-Hsin; Kuo, Po-Chih; Liu, Shih-Hsien; Wu, Wei

    2014-01-01

    Thermal behavior of biomass in torrefaction plays an important role in the operation of pretreatment. To understand the endothermic and/or exothermic characteristics of biomass in the course of torrefaction, an experimental system is conducted and two kinds of biomass (oil palm fiber and eucalyptus) are investigated. The results indicate that the thermal behavior is significantly influenced by the lignocellulosic composition in biomass and the torrefaction temperature. The thermal decomposition of hemicellulose is the dominant mechanism for oil palm fiber torrefied at 200 and 250 °C, whereas the thermal degradation of cellulose is crucial when the biomass is torrefied at 300 °C. Therefore, the heat of reaction of oil palm fiber increases with increasing torrefaction temperature. The torrefaction of eucalyptus is always endothermic, as a consequence of high cellulose contained in the biomass. It is less endothermic when the torrefaction temperature increases, presumably due to the char formation from cellulose thermal degradation and the exothermic lignin decomposition. As a whole, the values of the heat of reaction of the two samples are between −3.50 and 2.23 MJ/kg. The obtained results have provided a useful insight into the control of torrefaction operation and the design of torrefaction reactor. - Highlights: • Thermal behavior of oil palm fiber and eucalyptus in torrefaction is studied. • Thermal characteristic of biomass in torrefaction depends on lignocellulosic composition. • Heat of reaction of oil palm fiber increases with increasing torrefaction temperature. • Eucalyptus torrefaction is always endothermic because of high cellulose contained. • Torrefaction of eucalyptus is less endothermic when the torrefaction temperature increases

  20. Procedure for normalization of cDNA libraries

    Science.gov (United States)

    Bonaldo, Maria DeFatima; Soares, Marcelo Bento

    1997-01-01

    This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.

  1. cDNA microarray screening in food safety

    International Nuclear Information System (INIS)

    Roy, Sashwati; Sen, Chandan K.

    2006-01-01

    The cDNA microarray technology and related bioinformatics tools presents a wide range of novel application opportunities. The technology may be productively applied to address food safety. In this mini-review article, we present an update highlighting the late breaking discoveries that demonstrate the vitality of cDNA microarray technology as a tool to analyze food safety with reference to microbial pathogens and genetically modified foods. In order to bring the microarray technology to mainstream food safety, it is important to develop robust user-friendly tools that may be applied in a field setting. In addition, there needs to be a standardized process for regulatory agencies to interpret and act upon microarray-based data. The cDNA microarray approach is an emergent technology in diagnostics. Its values lie in being able to provide complimentary molecular insight when employed in addition to traditional tests for food safety, as part of a more comprehensive battery of tests

  2. Cloning and expression of cDNA coding for bouganin.

    Science.gov (United States)

    den Hartog, Marcel T; Lubelli, Chiara; Boon, Louis; Heerkens, Sijmie; Ortiz Buijsse, Antonio P; de Boer, Mark; Stirpe, Fiorenzo

    2002-03-01

    Bouganin is a ribosome-inactivating protein that recently was isolated from Bougainvillea spectabilis Willd. In this work, the cloning and expression of the cDNA encoding for bouganin is described. From the cDNA, the amino-acid sequence was deduced, which correlated with the primary sequence data obtained by amino-acid sequencing on the native protein. Bouganin is synthesized as a pro-peptide consisting of 305 amino acids, the first 26 of which act as a leader signal while the 29 C-terminal amino acids are cleaved during processing of the molecule. The mature protein consists of 250 amino acids. Using the cDNA sequence encoding the mature protein of 250 amino acids, a recombinant protein was expressed, purified and characterized. The recombinant molecule had similar activity in a cell-free protein synthesis assay and had comparable toxicity on living cells as compared to the isolated native bouganin.

  3. Eucalyptus gall wasp, Leptocybe invasa Fisher & La Salle (Insecta: Hymenoptera: Eulophidae), an emerging pest of eucalyptus in Florida

    Science.gov (United States)

    A new emerging pest of eucalyptus, Leptocybe invasa Fisher & La Salle, was first found in Italy but mistakenly identified as Aprostocetus sp.. This was followed by another report of an infestation from Turkey in early 2000. It was first formally described in 2004 from Australia as Leptocybe invasa a...

  4. Methanol production from Eucalyptus wood chips. Working document I. The Florida Eucalyptus energy farm: silvicultural methods and considerations

    Energy Technology Data Exchange (ETDEWEB)

    Fishkind, H.H.

    1982-04-01

    The silvicultural matrix within which the nation's first large scale wood energy plantation will develop is described in detail. The relevant literature reviewed is identified and distilled. The plantation history, site preparation, planting, species selection, maintenance and management, harvesting, and the Eucalyptus biomass production estimates are presented.

  5. cDNA encoding a polypeptide including a hevein sequence

    Energy Technology Data Exchange (ETDEWEB)

    Raikhel, Natasha V. (Okemos, MI); Broekaert, Willem F. (Dilbeek, BE); Chua, Nam-Hai (Scarsdale, NY); Kush, Anil (New York, NY)

    1993-02-16

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a pu GOVERNMENT RIGHTS This application was funded under Department of Energy Contract DE-AC02-76ER01338. The U.S. Government has certain rights under this application and any patent issuing thereon.

  6. Qualidade da celulose kraft-antraquinona de Eucalyptus dunnii plantado em cinco espaçamentos em relação ao Eucalyptus grandis e Eucalyptus saligna.

    Directory of Open Access Journals (Sweden)

    Giovanni Willer Ferreira

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 Este trabalho analisou o efeito de cinco espaçamentos de Eucalyptus dunnii (3 m x 1 m; 3 m x 1,5 m; 3 m x 2 m; 3 m x 3 m; 3 m x 4 m, para produção de celulose kraft-antraquinona, comparados com Eucalyptus grandis e Eucalyptus saligna (ambos no espaçamento 3 m x 2 m. Avaliaram-se a densidade básica e composição química da madeira original, analisando-se extrativos em diclorometano, pentosanas, lignina, solubilidade em NaOH8% e cinzas. Os cavacos foram submetidos a cozimento kraft-antraquinona com álcali ativo variando de 18,5 a 21% para obter um número kappa 17± 1,5; sulfidez a 8%, antraquinona base madeira 0,05%, relação licor/madeira 4:1, tempo até temperatura máxima 60 minutos, tempo à temperatura máxima 45 minutos. A celulose marrom resultante do cozimento kraft-antraquinona foi caracterizada e analisada em seus aspectos físico-químicos (rendimentos, rejeitos, número kappa, viscosidade intrínseca, alvura e solubilidade em NaOH5%. Uma parte desta sofreu refinação em moinho Jökro ao nível de 35°SR e, juntamente com a polpa não-refinada, foram submetidas a testes físico-mecânicos e óticos (resistência à tração, alongamento, estouro, rasgo, volume específico, alvura, resistência ao ar Gurley e ascensão capilar Klemm. O maior consumo de álcali ativo (20,5% ocorreu em espaçamento 3 m x 4 m, proporcionando maior degradação da polpa. Todos os espaçamentos caracterizados apresentaram viabilidade para a produção de celulose kraft-antraquinona, podendo proporcionar redução nos custos de produção de celulose, devido aos menores consumos apresentados de álcali ativo e madeira quando comparados com Eucalyptus grandis e Eucalyptus saligna. Os valores de extrativos e cinzas mostraram-se elevados para Eucalyptus dunnii, potencializando possíveis impactos em termos de incrustações e fechamento de circuito no processo fabril. Diante dos resultados

  7. cDNA library information - Dicty_cDB | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us Dicty_cDB cDNA library information Data detail Data name cDNA library information DOI 10.189...s Data item Description cDNA library name Names of cDNA libraries (AF, AH, CF, CH, FC, FC-IC, FCL, SF, SH, S...(C) 5) sexually fusion-competent KAX3 cells (Gamete phase) (F) cDNA library construction method How to construct cDNA library...dir) 2) Full-length cDNA libraries (oligocapped method)(fl) 3) Gamete-specific subtraction library (sub) cDNA library... construction protocol Link to the webpage describing the protocol for generating cDNA library Size

  8. Volume loss as a tool to assess kiln drying of eucalyptus wood

    OpenAIRE

    Batista,Djeison Cesar; Klitzke,Ricardo Jorge; Rocha,Márcio Pereira da; Muñiz,Graciela Inez Bolzon de; Batista,Tharcia Ribeiro

    2013-01-01

    In this study, we aimed to analyze the kiln drying quality of Eucalyptus grandis, Eucalyptus saligna and Eucalyptus dunnii woods with respect to volume loss. Wood from the three species was kiln dried together with the same drying schedule and conditions in a conventional-temperature pilot kiln. Three kinds of volume loss were evaluated: total - from saturated (initial) to machined (final) condition; shrinkage - from saturated to 10% moisture content; and machining - from 10% moisture content...

  9. Cloning and expression of human deoxycytidine kinase cDNA

    International Nuclear Information System (INIS)

    Chottiner, E.G.; Shewach, D.S.; Datta, N.S.; Ashcraft, E.; Gribbin, D.; Ginsburg, D.; Fox, I.H.; Mitchell, B.S.

    1991-01-01

    Deoxycytidine (dCyd) kinase is required for the phosphorylation of several deoxyribonucleosides and certain nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Detailed analysis of this enzyme has been limited, however, by its low abundance and instability. Using oligonucleotides based on primary amino acid sequence derived from purified dCyd kinase, the authors have screened T-lymphoblast cDNA libraries and identified a cDNA sequence that encodes a 30.5-kDa protein corresponding to the subunit molecular mass of the purified protein. Expression of the cDNA in Escherichia coli results in a 40-fold increase in dCyd kinase activity over control levels. Northern blot analysis reveals a single 2.8-kilobase mRNA expressed in T lymphoblasts at 5- to 10-fold higher levels than in B lymphoblasts, and decreased dCyd kinase mRNA levels are present in T-lymphoblast cell lines resistant to arabinofuranosylcytosine and dideoxycytidine. These findings document that this cDNA encodes the T-lymphoblast dCyd kinase responsible for the phosphorylation of dAdo and dGuo as well as dCyd and arabinofuranosylcytosine

  10. MOLECULAR CHARACTERIZATION OF ENDOCRINE DISRUPTION IN FISH USING CDNA ARRAYS.

    Science.gov (United States)

    We are developing cDNA macroarrays to measure the induction of gene expression in sheepshead minnows and largemouth bass exposed to anthropogenic chemicals that can mimic the action of endogenous hormones. For sheepshead minnows exposed in aqua, we observed similar genetic profil...

  11. A BIOINFORMATIC STRATEGY TO RAPIDLY CHARACTERIZE CDNA LIBRARIES

    Science.gov (United States)

    A Bioinformatic Strategy to Rapidly Characterize cDNA LibrariesG. Charles Ostermeier1, David J. Dix2 and Stephen A. Krawetz1.1Departments of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, & Institute for Scientific Computing, Wayne State Univer...

  12. NORMAL NASAL GENE EXPRESSION LEVELS USING CDNA ARRAY TECHNOLOGY

    Science.gov (United States)

    Normal Nasal Gene Expression Levels Using cDNA Array Technology. The nasal epithelium is a target site for chemically-induced toxicity and carcinogenicity. To detect and analyze genetic events which contribute to nasal tumor development, we first defined the gene expressi...

  13. Complete cDNA sequence coding for human docking protein

    Energy Technology Data Exchange (ETDEWEB)

    Hortsch, M; Labeit, S; Meyer, D I

    1988-01-11

    Docking protein (DP, or SRP receptor) is a rough endoplasmic reticulum (ER)-associated protein essential for the targeting and translocation of nascent polypeptides across this membrane. It specifically interacts with a cytoplasmic ribonucleoprotein complex, the signal recognition particle (SRP). The nucleotide sequence of cDNA encoding the entire human DP and its deduced amino acid sequence are given.

  14. cDNA, genomic sequence cloning and overexpression of ribosomal ...

    African Journals Online (AJOL)

    RPS16 of eukaryote is a component of the 40S small ribosomal subunit encoded by RPS16 gene and is also a homolog of prokaryotic RPS9. The cDNA and genomic sequence of RPS16 was cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using reverse transcription-polymerase chain ...

  15. Seed viability constants for Eucalyptus grandis Constantes de viabilidade para sementes de Eucalyptus grandis

    Directory of Open Access Journals (Sweden)

    Jussara Bertho Fantinatti

    2007-01-01

    Full Text Available This work aimed to analyse Eucalyptus grandis W.Hill ex Maiden seed behaviour, under controlled deterioration, and to estimate viability equation constants for the species. Seeds were harvested in the growing season of 1999, and the moisture contents were adjusted from 11.3% to a range between 1.2 and 18.1% at 25ºC. The subsamples were sealed into laminate aluminium-foil packets, for storage in incubators at 40, 50 and 65±0.5ºC. The seeds presented orthodox performance, in which the constants for predicting seed longevity of E. grandis were K E = 9.661, C W = 6.467, C H = 0.03498 and C Q = 0.0002330. The usual and inverse relationship between water content and seed longevity was also observed. The lowest moisture content limit for application of the viability equation at 65ºC was 4.9%, estimated under hygroscopic equilibrium with 23% of relative humidity in the storage environment.Este trabalho teve como objetivos verificar o desempenho de sementes de Eucalyptus grandis W.Hill ex Maiden, após a deterioração em condições controladas, e obter as constantes da equação de viabilidade. As sementes foram colhidas na safra de 1999, e a umidade foi ajustada de 11,3% para valores entre 1,2 e 18,1% a 25ºC As subamostras foram acondicionadas em embalagens de alumínio termossoldadas, armazenadas a 40, 50 e 65±0,5ºC. As sementes apresentaram um desempenho ortodoxo em relação ao armazenamento. As constantes para a predição da longevidade foram K E = 9,661, C W = 6,467, C H = 0,03498 e C Q = 0,0002330. Foi observada a relação inversa entre teor de água e longevidade. O limite inferior de grau de umidade, calculado para aplicação da equação a 65ºC, foi de 4,9%, estimativa obtida sob equilíbrio higroscópico com umidade relativa de 23% no ambiente de armazenamento.

  16. The influence of Eucalyptus plantations on the macrofauna associated with Salvinia auriculata in Southeast Brazil

    Directory of Open Access Journals (Sweden)

    CALLISTO M.

    2002-01-01

    Full Text Available The influence of Eucalyptus plantations on the structure and composition of macroinvertebrate communities associated with the aquatic fern Salvinia auriculata Aublet were investigated in a high altitude lake bordered by either secondary Atlantic forest or Eucalyptus plantations. Comparisons of the diversity of Chironomidae (Diptera, Insecta larvae in the littoral zone between these two vegetation types showed higher diversity of larvae in waters bordered by Eucalyptus. The results demonstrated that the predominance of carnivorous taxa among the macroinvertebrate fauna appears to be the major controlling factor for limiting diversity in lake areas bordered by Eucalyptus.

  17. The influence of Eucalyptus plantations on the macrofauna associated with Salvinia auriculata in Southeast Brazil

    Directory of Open Access Journals (Sweden)

    M. CALLISTO

    Full Text Available The influence of Eucalyptus plantations on the structure and composition of macroinvertebrate communities associated with the aquatic fern Salvinia auriculata Aublet were investigated in a high altitude lake bordered by either secondary Atlantic forest or Eucalyptus plantations. Comparisons of the diversity of Chironomidae (Diptera, Insecta larvae in the littoral zone between these two vegetation types showed higher diversity of larvae in waters bordered by Eucalyptus. The results demonstrated that the predominance of carnivorous taxa among the macroinvertebrate fauna appears to be the major controlling factor for limiting diversity in lake areas bordered by Eucalyptus.

  18. Transcriptionally active LTR retrotransposons in Eucalyptus genus are differentially expressed and insertionally polymorphic.

    Science.gov (United States)

    Marcon, Helena Sanches; Domingues, Douglas Silva; Silva, Juliana Costa; Borges, Rafael Junqueira; Matioli, Fábio Filippi; Fontes, Marcos Roberto de Mattos; Marino, Celso Luis

    2015-08-14

    In Eucalyptus genus, studies on genome composition and transposable elements (TEs) are particularly scarce. Nearly half of the recently released Eucalyptus grandis genome is composed by retrotransposons and this data provides an important opportunity to understand TE dynamics in Eucalyptus genome and transcriptome. We characterized nine families of transcriptionally active LTR retrotransposons from Copia and Gypsy superfamilies in Eucalyptus grandis genome and we depicted genomic distribution and copy number in two Eucalyptus species. We also evaluated genomic polymorphism and transcriptional profile in three organs of five Eucalyptus species. We observed contrasting genomic and transcriptional behavior in the same family among different species. RLC_egMax_1 was the most prevalent family and RLC_egAngela_1 was the family with the lowest copy number. Most families of both superfamilies have their insertions occurring Eucalyptus species. Using EST analysis and qRT-PCRs, we observed transcriptional activity in several tissues and in all evaluated species. In some families, osmotic stress increases transcript values. Our strategy was successful in isolating transcriptionally active retrotransposons in Eucalyptus, and each family has a particular genomic and transcriptional pattern. Overall, our results show that retrotransposon activity have differentially affected genome and transcriptome among Eucalyptus species.

  19. Propriedades de chapas tipo OSB, fabricadas com partículas acetiladas de madeiras de Eucalyptus grandis, Eucalyptus urophylla, Eucalyptus cloeziana e Pinus elliottii Properties of OSB manufactured with wood strands of Eucalyptus grandis, Eucalyptus urophylla, Eucalyptus cloeziana and Pinus elliottii

    Directory of Open Access Journals (Sweden)

    Carla Priscilla Távora Cabral

    2006-08-01

    Full Text Available Este trabalho teve como objetivo avaliar as propriedades de chapas de 0riented Strand Board (OSB, fabricadas com flocos de madeira de Eucalyptus grandis, Eucalyptus urophylla e Eucalyptus cloeziana e oriundas dos Municípios de Ponte Alta e Três Marias, no Estado de Minas Gerais. As massas específicas básicas das três espécies de eucaliptos das duas regiões foram, respectivamente: Ponte Alta (0,55; 0,61; e 0,70 g/cm³ e Três Márias (0,56; 0,58; e 0,69 g/cm³. Quando necessário, para manter as massas específicas das chapas próximas de 0,70 g/cm³ foram acrescentadas às partículas de madeira de eucalipto partículas de madeira de Pinus elliottii, oriundo da cidade de Viçosa, com massa específica de 0,45 g/cm³. Os flocos foram gerados nas dimensões médias de 90,00 x 20,00 x 0,46 mm. O adesivo utilizado foi o fenol-formaldeído, na proporção de 8% de sólidos, em relação à massa seca de partículas. Parte dos flocos de eucaliptos foram acetilados. As chapas foram prensadas à temperatura de 170 °C e 32 kgf/cm² de pressão. As propriedades das chapas foram determinadas segundo as normas da ABNT NBR 14810-3 (2002 e ASTM-D 1037 (1991. Os resultados foram comparados utilizando-se as normas ANSI/A - 208.1 (1993 e CSA 0437-93 (1993. As chapas contendo partículas acetiladas foram mais estáveis e adsorveram menos umidade. Na tração perpendicular, observou-se que as chapas contendo 100% de flocos acetilados apresentaram resultados inferiores ao estipulado pela norma CSA O437-0/93 (1993. A resistência ao arrancamento de parafuso, módulo de ruptura (paralelo e perpendicular e compressão longitudinal (perpendicular, foi reduzida pela acetilação nas chapas contendo 100% dos flocos acetilados. As espécies que apresentaram, numericamente, as maiores médias para resistência mecânica foram: Eucalyptus grandis não acetilado (dureza Janka e Eucalyptus cloeziana misturado com Pinus sp (módulo de ruptura. Somente a resistência

  20. Species discrimination, population structure and linkage disequilibrium in Eucalyptus camaldulensis and Eucalyptus tereticornis using SSR markers.

    Directory of Open Access Journals (Sweden)

    Shanmugapriya Arumugasundaram

    Full Text Available Eucalyptus camaldulensis and E. tereticornis are closely related species commonly cultivated for pulp wood in many tropical countries including India. Understanding the genetic structure and linkage disequilibrium (LD existing in these species is essential for the improvement of industrially important traits. Our goal was to evaluate the use of simple sequence repeat (SSR loci for species discrimination, population structure and LD analysis in these species. Investigations were carried out with the most common alleles in 93 accessions belonging to these two species using 62 SSR markers through cross amplification. The polymorphic information content (PIC ranged from 0.44 to 0.93 and 0.36 to 0.93 in E. camaldulensis and E. tereticornis respectively. A clear delineation between the two species was evident based on the analysis of population structure and species-specific alleles. Significant genotypic LD was found in E. camaldulensis, wherein out of 135 significant pairs, 17 pairs showed r(2≥0.1. Similarly, in E. tereticornis, out of 136 significant pairs, 18 pairs showed r(2≥0.1. The extent of LD decayed rapidly showing the significance of association analyses in eucalypts with higher resolution markers. The availability of whole genome sequence for E. grandis and the synteny and co-linearity in the genome of eucalypts, will allow genome-wide genotyping using microsatellites or single nucleotide polymorphims.

  1. The function analysis of full-length cDNA sequence from IRM-2 mouse cDNA library

    International Nuclear Information System (INIS)

    Wang Qin; Liu Xiaoqiu; Xu Chang; Du Liqing; Sun Zhijuan; Wang Yan; Liu Qiang; Song Li; Li Jin; Fan Feiyue

    2013-01-01

    Objective: To identify the function of full-length cDNA sequence from IRM-2 mouse cDNA library. Methods: Full-length cDNA products were amplified by PCR from IRM-2 mouse cDNA library according to twenty-one pieces of expressed sequence tag. The expression of full-length cDNAs were detected after mouse embryonic fibroblasts were exposed to 6.5 Gy γ-ray radiation. And the effect on the growth of radiosensitivity cells AT5B1VA transfected with full-length cDNAs was investigated. Results: The expression of No.4, 5 and 2 full-length cDNAs from IRM-2 mouse were higher than that of parental ICR and 615 mouse after mouse embryonic fibroblasts irradiated with γ-ray radiation. And the survival rate of AT5B1VA cells transfected with No.4, 5 and 2 full-length cDNAs was high. Conclusion: No.4, 5 and 2 full-length cDNAs of IRM-2 mouse are of high radioresistance. (authors)

  2. Genes encoding enzymes of the lignin biosynthesis pathway in Eucalyptus

    Directory of Open Access Journals (Sweden)

    Ricardo Harakava

    2005-01-01

    Full Text Available Eucalyptus ESTs libraries were screened for genes involved in lignin biosynthesis. This search was performed under the perspective of recent revisions on the monolignols biosynthetic pathway. Eucalyptus orthologues of all genes of the phenylpropanoid pathway leading to lignin biosynthesis reported in other plant species were identified. A library made with mRNAs extracted from wood was enriched for genes involved in lignin biosynthesis and allowed to infer the isoforms of each gene family that play a major role in wood lignin formation. Analysis of the wood library suggests that, besides the enzymes of the phenylpropanoids pathway, chitinases, laccases, and dirigent proteins are also important for lignification. Colocalization of several enzymes on the endoplasmic reticulum membrane, as predicted by amino acid sequence analysis, supports the existence of metabolic channeling in the phenylpropanoid pathway. This study establishes a framework for future investigations on gene expression level, protein expression and enzymatic assays, sequence polymorphisms, and genetic engineering.

  3. [Preparation of the cDNA microarray on the differential expressed cDNA of senescence-accelerated mouse's hippocampus].

    Science.gov (United States)

    Cheng, Xiao-Rui; Zhou, Wen-Xia; Zhang, Yong-Xiang

    2006-05-01

    Alzheimer' s disease (AD) is the most common form of dementia in the elderly. AD is an invariably fatal neurodegenerative disorder with no effective treatment. Senescence-accelerated mouse prone 8 (SAMP8) is a model for studying age-related cognitive impairments and also is a good model to study brain aging and one of mouse model of AD. The technique of cDNA microarray can monitor the expression levels of thousands of genes simultaneously and can be used to study AD with the character of multi-mechanism, multi-targets and multi-pathway. In order to disclose the mechanism of AD and find the drug targets of AD, cDNA microarray containing 3136 cDNAs amplified from the suppression subtracted cDNA library of hippocampus of SAMP8 and SAMR1 was prepared with 16 blocks and 14 x 14 pins, the housekeeping gene beta-actin and G3PDH as inner conference. The background of this microarray was low and unanimous, and dots divided evenly. The conditions of hybridization and washing were optimized during the hybridization of probe and target molecule. After the data of hybridization analysis, the differential expressed cDNAs were sequenced and analyzed by the bioinformatics, and some of genes were quantified by the real time RT-PCR and the reliability of this cDNA microarray were validated. This cDNA microarray may be the good means to select the differential expressed genes and disclose the molecular mechanism of SAMP8's brain aging and AD.

  4. Did Eucalyptus contribute to environment degradation? Implications from a dispute on causes of severe drought in Yunnan and Guizhou, China

    OpenAIRE

    WenJun Zhang

    2012-01-01

    Various viewpoints were proposed to explain the causes of recent years' severe drought occurred in Yunnan and Guizhou, China. In general there are two parties of viewpoints, the Eucalyptus cause and climate change cause. I think Yunnan-Guizhou drought has been mainly caused by abnormal climate change. Eucalyptus was not significant in the formation of Yunnan-Guizhou drought. However, the forestation effect of Eucalyptus in China was not good. Environment quality and biodiversity in Eucalyptus...

  5. THE Eucalyptus sp. AGE PLANTATIONS INFLUENCING THE CARBON STOCKS

    Directory of Open Access Journals (Sweden)

    Charlote Wink

    2013-06-01

    Full Text Available http://dx.doi.org/10.5902/198050989279The tree growth and biomass accumulation, as well as the maintenance of forest residue at the soil surface can act in the removal of carbon from the atmosphere through the cycling process of plant material. The objective was to study the influence of Eucalyptus sp. Plantations with 20, 44 and 240 months of age on the variation of carbon in soil and biomass. The carbon in the soil depth was determined by CHNS auto-analyzer and carbon in the vegetation was determined by the biomass in each forest, considering a factor of 0.45 of the dry mass. We determined the density and particle size distribution of soil. For the comparison between plantations, there was analysis of variance and comparison of means of carbon in vegetation and soil, considering the 5% level of probability. The carbon content and stock in the soil were low, indicating that a natural feature of the category of Paleuldt, or the growth of eucalyptus forests, replacing the field native vegetation did not aggregate a significant increase in the carbon. Although, there was a significant increase carbon in aboveground biomass. It includes forest biomass and litter. So, despite the values ​​of carbon stocks are low, it identified a greater average total in the soil compared to the stock aboveground. Furthermore, this increase aboveground (tree and litter compartments can be considered significant between the eucalyptus plantations of different ages.

  6. History and Status of Eucalyptus Improvement in Florida

    Directory of Open Access Journals (Sweden)

    Donald L. Rockwood

    2012-01-01

    Full Text Available The first organized Eucalyptus research in Florida was begun by the Florida Forests Foundation in 1959 in southern Florida. This research was absorbed by the USDA Forest Service and the Florida Division of Forestry in 1968. In the early 1970s, the Eucalyptus Research Cooperative formed to provide additional support emphasized E. grandis, E. robusta, E. camaldulensis, and E. tereticornis and developed cultural practices for commercial plantations in southern Florida. In 1978, this cooperative united with the Hardwood Research Cooperative at North Carolina State University until 1985 when the 14-year effort ended after three severe freezes from 1983 to 1985. Eucalyptus planting and research were continued with a Florida-wide focus by the University of Florida and collaborators starting in 1980. The collective accomplishments in terms of genetic resources and commercial planting are summarized. For example, fast-growing, freeze-resilient E. grandis seedlings are produced by advanced generation seed orchards, five E. grandis cultivars are commercially available, as are E. amplifolia and Corymbia torelliana seeds. Genetic improvement of these and other species is ongoing due to beneficial collaborations. Short Rotation Woody Crop systems are promising for increasing productivity and extending uses beyond conventional pulpwood to applications such as windbreaks, dendroremediation, and energy wood.

  7. Unintentional exposure of young children to camphor and eucalyptus oils.

    Science.gov (United States)

    Flaman, Z; Pellechia-Clarke, S; Bailey, B; McGuigan, M

    2001-02-01

    Essential oils, such as camphorated and eucalyptus oils, are volatile oils that can be absorbed by mouth and through the skin; if ingested orally by children, they can be harmful, even life-threatening. To determine the frequency of essential oil ingestion among children in Toronto, Ontario. Charts from December 1995 through March 1997 at the Ontario Regional Poison Information Centre, The Hospital for Sick Children, Toronto were reviewed to collect information on calls about essential oil ingestion, and a search of MEDLINE articles from 1966 to 1998 was conducted using the key words: 'camphor', 'eucalyptus', 'paediatric', and 'poisoning'. Callers to the Poison Information Centre reported that 251 children had ingested an essential oil or product: eucalyptus oil 50 children; camphorated oil 18 children; VapAir (Drug Trading, Canada) vaporizing liquid 93 children; and Vicks VaporRub (Procter & Gamble, Canada) 90 children. The most common symptoms were cough, vomiting and cough associated with vomiting. Two children had seizures but recovered. The MEDLINE search found 18 reports of paediatric ingestion of the oils or oil products. The main symptoms were vomiting, lethargy, coma and seizures. One child died. Although widely used by health care consumers, essential oils and the products that contain them can be harmful when ingested by children. Further education for parents and other caregivers about the risks involved in exposure to these products is required.

  8. Selective Herbicides for Cultivation of Eucalyptus urograndis Clones

    Directory of Open Access Journals (Sweden)

    Patrick J. Minogue

    2015-01-01

    Full Text Available Competition control is essential for successful eucalyptus plantation establishment, yet few selective herbicides have been identified. Five herbicides, flumioxazin, imazamox, imazapic, oxyfluorfen, and sulfometuron methyl, were evaluated for selective weed control in the establishment of genetically modified frost tolerant Eucalyptus urograndis clones. Herbicides were applied at two or three rates, either before or after weed emergence, and compared to a nontreated control and to near-complete weed control obtained with glyphosate directed sprays. Applications prior to weed emergence were most effective for weed control and, with the exception of imazapic, all resulted in enhanced eucalyptus growth relative to the nontreated control. Among postemergent treatments, only imazamox enhanced stem volume. Among selective herbicide treatments, preemergent 2240 g ha−1 oxyfluorfen produced the best growth response, resulting in stem volume index that was 860% greater than the nontreated control, although only 15% of the volume index obtained with near-complete weed control. Imazapic was the most phytotoxic of all herbicides, resulting in 40% mortality when applied preemergent. Survival was 100% for all other herbicide treatments. This research found the previously nontested herbicides imazamox and imazapic to be effective for selective weed control and refined application rate and timing of five herbicides for use in clonal plantations.

  9. Energy-Based Evaluations on Eucalyptus Biomass Production

    Directory of Open Access Journals (Sweden)

    Thiago L. Romanelli

    2012-01-01

    Full Text Available Dependence on finite resources brings economic, social, and environmental concerns. Planted forests are a biomass alternative to the exploitation of natural forests. In the exploitation of the planted forests, planning and management are key to achieve success, so in forestry operations, both economic and noneconomic factors must be considered. This study aimed to compare eucalyptus biomass production through energy embodiment of anthropogenic inputs and resource embodiment including environmental contribution (emergy for the commercial forest in the Sao Paulo, Brazil. Energy analyses and emergy synthesis were accomplished for the eucalyptus production cycles. It was determined that emergy synthesis of eucalyptus production and sensibility analysis for three scenarios to adjust soil acidity (lime, ash, and sludge. For both, energy analysis and emergy synthesis, harvesting presented the highest input demand. Results show the differences between energy analysis and emergy synthesis are in the conceptual underpinnings and accounting procedures. Both evaluations present similar trends and differ in the magnitude of the participation of an input due to its origin. For instance, inputs extracted from ores, which represent environmental contribution, are more relevant for emergy synthesis. On the other hand, inputs from industrial processes are more important for energy analysis.

  10. High-throughput screening of suppression subtractive hybridization cDNA libraries using DNA microarray analysis

    CSIR Research Space (South Africa)

    Van den Berg, N

    2004-11-01

    Full Text Available Efficient construction of cDNA libraries enriched for differentially expressed transcripts is an important first step in many biological investigations. We present a quantitative procedure for screening cDNA libraries constructed by suppression...

  11. CANONICAL CORRELATION OF PHYSICAL AND CHEMICAL CHARACTERISTICS OF THE WOOD OF Eucalyptus grandis AND Eucalyptus saligna CLONES

    Directory of Open Access Journals (Sweden)

    Paulo Fernando Trugilho

    2003-01-01

    Full Text Available The analysis of canonical correlation measures the existence and the intensity of the association between two groups of variables. The research objectified to evaluate thecanonical correlation between chemical and physical characteristics and fiber dimensional ofwood of Eucalyptus grandis and Eucalyptus saligna clones, verifying the interdependenceamong the groups of studied variables. The analysis indicated that the canonical correlationswere high and that in two cases the first and second pair were significant at the level of 1% ofprobability. The analysis of canonical correlation showed that the groups are notindependent. The intergroup associations indicated that the wood of high insoluble lignin contentand low ash content is associated with the high radial and tangential contraction and highbasic density wood.

  12. Speciation and distribution of Botryosphaeria spp. on native and introduced Eucalyptus trees in Australia and South Africa

    NARCIS (Netherlands)

    Slippers, B.; Fourie, G.; Crous, P.W.; Coutinho, T.A.; Wingfield, B.D.; Carnegie, A.J.; Wingfield, M.J.

    2004-01-01

    Botryosphaeria spp. are important canker and die-back pathogens that affect Eucalyptus spp. They also occur endophytically in Eucalyptus leaves and stems. For the purpose of this study, Botryosphaeria strains were isolated from diseased and symptomless Eucalyptus material from Australia and South

  13. Did Eucalyptus contribute to environment degradation? Implications from a dispute on causes of severe drought in Yunnan and Guizhou, China

    Directory of Open Access Journals (Sweden)

    WenJun Zhang

    2012-06-01

    Full Text Available Various viewpoints were proposed to explain the causes of recent years' severe drought occurred in Yunnan and Guizhou, China. In general there are two parties of viewpoints, the Eucalyptus cause and climate change cause. I think Yunnan-Guizhou drought has been mainly caused by abnormal climate change. Eucalyptus was not significant in the formation of Yunnan-Guizhou drought. However, the forestation effect of Eucalyptus in China was not good. Environment quality and biodiversity in Eucalyptus plantation forests has been degrading in last decades. Enhancement of alleopathy of Eucalyptus trees under drought conditions would partly contribute to biodiversity reduction and environment degradation in Eucalyptus plantation forests. For existing Eucalyptus plantation forests of Yunnan, I suggest that some improvement measures should be adopted. Artificial weeding and cleaning in Eucalyptus plantation forests should be banned. Density of Eucalyptus trees needs to be reduced. Biodiversity should be artificially improved in Eucalyptus plantation forests. In the future, the mountains and lands with better vegetation cover must not be reclaimed for Eucalyptus planting. Eucalyptus plantation forests should be made in barren mountains and lands with poor biodiversity.

  14. cDNA - ASTRA | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available ontents List of cDNA in locus Data file File name: astra_cdna.zip File URL: ftp://ftp.biosciencedbc.jp/archive/astra/LATEST/astra_cdn...a.zip File size: 3.3 MB Simple search URL http://togodb.biosciencedbc.jp/togodb/view/astra_cdna...n, Department of Molecular Genetics, National Institute of Agrobiological Sciences (Kikuchi et al., 2003; ftp://cdna

  15. In vitro anticancer properties of selected Eucalyptus species.

    Science.gov (United States)

    Bhuyan, Deep Jyoti; Sakoff, Jennette; Bond, Danielle R; Predebon, Melanie; Vuong, Quan V; Chalmers, Anita C; van Altena, Ian A; Bowyer, Michael C; Scarlett, Christopher J

    2017-08-01

    In spite of the recent advancements in oncology, the overall survival rate for pancreatic cancer has not improved over the last five decades. Eucalypts have been linked with cytotoxic and anticancer properties in various studies; however, there is very little scientific evidence that supports the direct role of eucalypts in the treatment of pancreatic cancer. This study assessed the anticancer properties of aqueous and ethanolic extracts of four Eucalyptus species using an MTT assay. The most promising extracts were further evaluated using a CCK-8 assay. Apoptotic studies were performed using a caspase 3/7 assay in MIA PaCa-2 cells. The aqueous extract of Eucalyptus microcorys leaf and the ethanolic extract of Eucalyptus microcorys fruit inhibited the growth of glioblastoma, neuroblastoma, lung and pancreatic cancer cells by more than 80% at 100 μg/mL. The E. microcorys and Eucalyptus saligna extracts showed lower GI 50 values than the ethanolic Eucalyptus robusta extract in MIA PaCa-2 cells. Aqueous E. microcorys leaf and fruit extracts at 100 μg/mL exerted significantly higher cell growth inhibition in MIA PaCa-2 cells than other extracts (p  0.05) were observed in aqueous E. microcorys leaf (86.05 ± 4.75 μg/mL) and fruit (64.66 ± 15.97 μg/mL) and ethanolic E. microcorys leaf (79.30 ± 29.45 μg/mL) extracts in MIA PaCa-2 cells using the CCK-8 assay. Caspase 3/7-mediated apoptosis and morphological changes of cells were also witnessed in MIA PaCa-2 cells after 24 h of treatment with the extracts. This study highlighted the significance of E. microcorys as an important source of phytochemicals with efficacy against pancreatic cancer cells. Further studies are warranted to purify and structurally identify individual compounds and elucidate their mechanisms of action for the development of more potent and specific chemotherapeutic agents for pancreatic cancer.

  16. Molecular cloning of growth hormone encoding cDNA of Indian

    Indian Academy of Sciences (India)

    A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in ...

  17. Phylogenetic reassessment of Mycosphaerella spp. and their anamorphs occurring on Eucalyptus

    NARCIS (Netherlands)

    Crous, P.W.; Groenewald, J.Z.; Mansilla, J.P.; Hunter, G.C.; Wingfield, M.J.

    2004-01-01

    Species of Eucalyptus, mostly native to Australia, are widely planted as exotics in the tropics and Southern Hemisphere. These plantations represent an important source of fuel-wood, structural timber and pulp. Eucalyptus plantations are, however, vulnerable to infection by pathogens, including

  18. Management of Eucalyptus plantations influence small mammals’ density: evidences from Southern Europe

    NARCIS (Netherlands)

    Teixeira, D; Carrilho, M; Mexia, T; Kobel, M; Ferreira Dos Santos, M.J.; Santos-Reis, Margarida; Rosalino, Luis Miguel

    2017-01-01

    Forestry plantations, and particularly those of exotic Eucalyptus, are important man-made systems in Europe, and especially in Portugal, where these represent now the largest fraction of forested areas. Eucalyptus plantations may have impacts on vertebrate communities in Europe; however, these have

  19. Eucalyptus beyond its native range: Environmental issues in exotic bioenergy plantations

    Science.gov (United States)

    John A. Stanturf; Eric D. Vance; Thomas R. Fox; Matias Kirst

    2013-01-01

    The genus Eucalyptus is native to Australia and Indonesia but has been widely planted in many countries. Eucalyptus has proven to be particularly successful in tropical and subtropical regions. Several species are also successful in some temperate regions, but problems with sudden and severe frosts pose limitations. Current...

  20. Processed eucalyptus trees as a substrate component for greenhouse crop production

    Science.gov (United States)

    Fast growing eucalyptus species are selected for commercial plantings worldwide and are harvested for a variety of uses. Eucalyptus plantings in south Florida are harvested for landscape mulch production, yet this material may have potential as a container substrate for horticulture crop production....

  1. Effect of surfactant concentration on the spreading properties of pesticide droplets on Eucalyptus leaves

    Science.gov (United States)

    The area wetted by 500-µm diameter droplets of pesticide and deionised water placed at different positions on Eucalyptus urophylla × E. grandis (E.u × E.g) and Eucalyptus tereticornis (E.t) leaves was determined at an air temperature of 30 °C and a relative humidity of 60%. Dimethyl dichlorovinyl ph...

  2. Herbicide site preparation and release options for eucalyptus plantation establishment in the western gulf

    Science.gov (United States)

    Michael A. Blazier; John Johnson; Eric L. Taylor; Brad Osbon

    2012-01-01

    Cold-tolerant species of eucalyptus (Eucalyptus spp.) are increasingly grown in the Western Gulf region as short-rotation pulpwood feedstock. Operational chemical suppression of competing vegetation has been relatively costly and inefficient because it requires frequent applications of glyphosate applied via backpack sprayers. A series of studies...

  3. Effects of irrigation on water use and water use efficiency in two fast growing Eucalyptus plantations

    Science.gov (United States)

    Robert M. Hubbard; Jose Stape; Michael G. Ryan; Auro C. Almeida; Juan Rojas

    2010-01-01

    Eucalyptus plantations occupy almost 20 million ha worldwide and exceed 3.7 million ha in Brazil alone. Improved genetics and silviculture have led to as much as a three-fold increase in productivity in Eucalyptus plantations in Brazil and the large land area occupied by these highly productive ecosystems raises concern over their...

  4. Projecting potential adoption of genetically engineered freeze-tolerant Eucalyptus in the United States

    Science.gov (United States)

    David N. Wear; Ernest Dixon IV; Robert C. Abt; Navinder Singh

    2015-01-01

    Development of commercial Eucalyptus plantations has been limited in the United States because of the species’ sensitivity to freezing temperatures. Recently developed genetically engineered clones of a Eucalyptus hybrid, which confer freeze tolerance, could expand the range of commercial plantations. This study explores how...

  5. The cDNA sequence of a neutral horseradish peroxidase.

    Science.gov (United States)

    Bartonek-Roxå, E; Eriksson, H; Mattiasson, B

    1991-02-16

    A cDNA clone encoding a horseradish (Armoracia rusticana) peroxidase has been isolated and characterized. The cDNA contains 1378 nucleotides excluding the poly(A) tail and the deduced protein contains 327 amino acids which includes a 28 amino acid leader sequence. The predicted amino acid sequence is nine amino acids shorter than the major isoenzyme belonging to the horseradish peroxidase C group (HRP-C) and the sequence shows 53.7% identity with this isoenzyme. The described clone encodes nine cysteines of which eight correspond well with the cysteines found in HRP-C. Five potential N-glycosylation sites with the general sequence Asn-X-Thr/Ser are present in the deduced sequence. Compared to the earlier described HRP-C this is three glycosylation sites less. The shorter sequence and fewer N-glycosylation sites give the native isoenzyme a molecular weight of several thousands less than the horseradish peroxidase C isoenzymes. Comparison with the net charge value of HRP-C indicates that the described cDNA clone encodes a peroxidase which has either the same or a slightly less basic pI value, depending on whether the encoded protein is N-terminally blocked or not. This excludes the possibility that HRP-n could belong to either the HRP-A, -D or -E groups. The low sequence identity (53.7%) with HRP-C indicates that the described clone does not belong to the HRP-C isoenzyme group and comparison of the total amino acid composition with the HRP-B group does not place the described clone within this isoenzyme group. Our conclusion is that the described cDNA clone encodes a neutral horseradish peroxidase which belongs to a new, not earlier described, horseradish peroxidase group.

  6. Behavior of Eucalyptus urophylla and Eucalyptus citriodora Seedlings Grown in Soil Contaminated by Arsenate

    Directory of Open Access Journals (Sweden)

    Roseli Freire Melo

    2016-01-01

    Full Text Available ABSTRACT Persistent areas of tailings and deposits from coal and gold mining may present high levels of arsenic (As, mainly in the arsenate form, endangering the environment and human health. The establishment of vegetation cover is a key step to reclaiming these environments. Thus, this study aimed to evaluate the potential of Eucalyptus urophylla and E. citriodora seedlings for use in phytoremediation programs of arsenate-contaminated areas. Soil samples were incubated at increasing rates (0, 50, 100, 200 and 400 mg dm-3 of arsenic (arsenate form, using Na2HAsO4 for 15 days. The seedlings were produced in a substrate (vermiculite + sawdust and were transplanted to the pots with soil three months after seed germination. The values of plant height and diameter were taken during transplanting and 30, 60 and 90 days after transplanting. In the last evaluation, the total leaf area and biomass of shoots and roots were also determined. The values of available As in soil which caused a 50 % dry matter reduction (TS50%, the As translocation index (TI from the roots to the shoot of the plants, and its bioconcentration factor (BF were also calculated. Higher levels of arsenate in the soil significantly reduced the dry matter production of roots and shoots and the height of both species, most notably in E. urophylla plants. The highest levels of As were found in the root, with higher values for E. citriodora (ranging from 253.86 to 400 mg dm-3. The TI and BF were also reduced with As doses, but the values found in E. citriodora were significantly higher than in E. urophylla. E. citriodora plants presented a higher capacity to tolerate As and translocate it to the shoot than E. urophylla. Although these species cannot be considered as hyperaccumulators of As, E. citriodora presented the potential to be used in phytoremediation programs in arsenate-contaminated areas due to the long-term growth period of this species.

  7. CDNA encoding a polypeptide including a hevein sequence

    Science.gov (United States)

    Raikhel, Natasha V.; Broekaert, Willem F.; Chua, Nam-Hai; Kush, Anil

    1995-03-21

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

  8. cDNA encoding a polypeptide including a hevein sequence

    Energy Technology Data Exchange (ETDEWEB)

    Raikhel, N.V.; Broekaert, W.F.; Chua, N.H.; Kush, A.

    2000-07-04

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74--79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

  9. Brain cDNA clone for human cholinesterase

    International Nuclear Information System (INIS)

    McTiernan, C.; Adkins, S.; Chatonnet, A.; Vaughan, T.A.; Bartels, C.F.; Kott, M.; Rosenberry, T.L.; La Du, B.N.; Lockridge, O.

    1987-01-01

    A cDNA library from human basal ganglia was screened with oligonucleotide probes corresponding to portions of the amino acid sequence of human serum cholinesterase. Five overlapping clones, representing 2.4 kilobases, were isolated. The sequenced cDNA contained 207 base pairs of coding sequence 5' to the amino terminus of the mature protein in which there were four ATG translation start sites in the same reading frame as the protein. Only the ATG coding for Met-(-28) lay within a favorable consensus sequence for functional initiators. There were 1722 base pairs of coding sequence corresponding to the protein found circulating in human serum. The amino acid sequence deduced from the cDNA exactly matched the 574 amino acid sequence of human serum cholinesterase, as previously determined by Edman degradation. Therefore, our clones represented cholinesterase rather than acetylcholinesterase. It was concluded that the amino acid sequences of cholinesterase from two different tissues, human brain and human serum, were identical. Hybridization of genomic DNA blots suggested that a single gene, or very few genes coded for cholinesterase

  10. cDNA encoding a polypeptide including a hevein sequence

    Energy Technology Data Exchange (ETDEWEB)

    Raikhel, N.V.; Broekaert, W.F.; Chua, N.H.; Kush, A.

    1999-05-04

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74--79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli. 12 figs.

  11. cDNA encoding a polypeptide including a hevein sequence

    Energy Technology Data Exchange (ETDEWEB)

    Raikhel, Natasha V. (Okemos, MI); Broekaert, Willem F. (Dilbeek, BE); Chua, Nam-Hai (Scarsdale, NY); Kush, Anil (New York, NY)

    1999-05-04

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

  12. cDNA encoding a polypeptide including a hevein sequence

    Energy Technology Data Exchange (ETDEWEB)

    Raikhel, N.V.; Broekaert, W.F.; Chua, N.H.; Kush, A.

    1995-03-21

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1,018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74--79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli. 11 figures.

  13. Eucalyptus Tree: A Potential Source of Cryptococcus neoformans in Egyptian Environment

    Directory of Open Access Journals (Sweden)

    Mahmoud Elhariri

    2016-01-01

    Full Text Available In Egypt, the River Red Gum (Eucalyptus camaldulensis is a well-known tree and is highly appreciated by the rural and urban dwellers. The role of Eucalyptus trees in the ecology of Cryptococcus neoformans is documented worldwide. The aim of this survey was to show the prevalence of C. neoformans during the flowering season of E. camaldulensis at the Delta region in Egypt. Three hundred and eleven samples out of two hundred Eucalyptus trees, including leaves, flowers, and woody trunks, were collected from four governorates in the Delta region. Thirteen isolates of C. neoformans were recovered from Eucalyptus tree samples (4.2%. Molecular identification of C. neoformans was done by capsular gene specific primer CAP64 and serotype identification was done depending on LAC1 gene. This study represents an update on the ecology of C. neoformans associated with Eucalyptus tree in Egyptian environment.

  14. A comparison of the effectiveness of chloroform and eucalyptus oil in dissolving root canal sealers.

    Science.gov (United States)

    Schäfer, Edgar; Zandbiglari, Tannaz

    2002-05-01

    The solubility of 8 different root canal sealers in chloroform and in eucalyptus oil was compared. For standardized samples (n=12), ring molds were filled with mixed sealers based on epoxy resin, silicone, calcium hydroxide, zinc oxide-eugenol, glass ionomer, and polyketone. These samples were immersed in chloroform or eucalyptus oil for 30 seconds, 1 minute, 2 minutes, 5 minutes, 10 minutes, and 20 minutes. Then, the mean weight loss was determined and statistically analyzed. With the exception of the silicone, all the sealers showed significantly higher solubilities (P <.05) in chloroform than in eucalyptus oil. Epoxy resin was the most soluble sealer in chloroform. In eucalyptus oil, calcium hydroxide, and zinc oxide-eugenol showed the highest solubility. Under the conditions of this study, chloroform was a far more effective solvent of root canal sealers than eucalyptus oil. Because of the potential hazards of chloroform, further studies on the dissolution of root canal sealers in different solvents seem to be necessary.

  15. Pretreatment of Eucalyptus in biphasic system for furfural production and accelerated enzymatic hydrolysis.

    Science.gov (United States)

    Zhang, Xiudong; Bai, Yuanyuan; Cao, Xuefei; Sun, Runcang

    2017-08-01

    Herein, an efficient biphasic pretreatment process was developed to improve the production of furfural (FF) and glucose from Eucalyptus. The influence of formic acid and NaCl on FF production from xylose in water and various biphasic systems was investigated. Results showed that the addition of formic acid and NaCl significantly promoted the FF yield, and the biphasic system of MIBK (methyl isobutyl ketone)/water exhibited the best performance for FF production. Then the Eucalyptus was pretreated in the MIBK/water system, and a maximum FF yield of 82.0% was achieved at 180°C for 60min. Surface of the pretreated Eucalyptus became relatively rough and loose, and its crystallinity index increased obviously due to the removal of hemicelluloses and lignin. The pretreated Eucalyptus samples showed much higher enzymatic hydrolysis rates (26.2-70.7%) than the raw Eucalyptus (14.5%). Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Control of Passion Fruit Fungal Diseases Using Essential Oils Extracted from Rosemary (Rosmarinus officinalis) and Eucalyptus (Eucalyptus agglomerata) in Egerton University Main Campus Njoro, Kenya.

    Science.gov (United States)

    Waithaka, Paul Njenga; Gathuru, Eliud Mugu; Githaiga, Benson Muriuki; Kimani, Salome Nduta

    2017-01-01

    Growth of fruits which form an important part of human diet has been jeopardized by the many fungal diseases that are present today. This study was conceived to isolate the most common fungal pathogens in passion fruits. Fungi were isolated using potato dextrose agar in addition to characterization using morphological, cultural, and biochemical means. Extraction of essential oils from rosemary ( Rosmarinus officinalis ) and eucalyptus ( Eucalyptus agglomerata ) was done. Before carrying the sensitivity test of essential oils to the fungal isolates, constituents of the essential oils were determined. The most common fungal pathogens isolated from passion fruits were Alternaria spp. (45%), Fusarium spp. (22%), Colletotrichum spp. (17%), and Penicillium spp. (16%). There was a relationship between heating time and yield of essential oils in rosemary ( r = 0.99) and eucalyptus ( r = 0.99). Conversely, there was no significant difference in the amount of essential oils produced by rosemary and eucalyptus ( P = 0.08). Furthermore, there was a significant difference in growth inhibition of the fungal pathogens between essential oils from rosemary and eucalyptus ( P = 0.000438). Fungal pathogens isolated from passion fruits can be controlled using essential oils from rosemary and eucalyptus. The oils need to be produced in large scale.

  17. RESISTÊNCIA BIOLÓGICA DA MADEIRA TRATADA DE Eucalyptus grandis E Eucalyptus cloeziana A FUNGOS APODRECEDORES EM ENSAIOS DE LABORATÓRIO

    Directory of Open Access Journals (Sweden)

    Magnos Alan Vivian

    2015-01-01

    Full Text Available The present study aimed to evaluate the biological resistance of treated wood from Eucalyptus grandis and Eucalyptus cloeziana under the action of biodeteriorative organisms in laboratory testing. Thus, we used trees of Eucalyptus grandis and Eucalyptus cloeziana, both 16 years old, which was converted into planks and subjected to preservative treatment in an autoclave with chromate copper arsenate (CCA. Then, it was made the specimens for the conduct of accelerated decay test, as recommended by ASTM. From the results, it was observed for the fungus Trametes versicolor that the preservative treatment was effective in reducing the biological degradation of the wood of the two species, with reduced mass loss in 35.17 and 82.31% for wood Eucalyptus grandis and Eucalyptus cloeziana, respectively, as for the fungus Gloeophyllum trabeum mass loss was reduced by 6.79 and 96.65%, compared to the control. Based on the conditions of realization of the present study, it was observed that preservative treatment with CCA is effective in the increasing the biological resistance of the wood under the action of fungi Trametes versicolor and Gloeophyllum trabeum.

  18. [cDNA library construction from panicle meristem of finger millet].

    Science.gov (United States)

    Radchuk, V; Pirko, Ia V; Isaenkov, S V; Emets, A I; Blium, Ia B

    2014-01-01

    The protocol for production of full-size cDNA using SuperScript Full-Length cDNA Library Construction Kit II (Invitrogen) was tested and high quality cDNA library from meristematic tissue of finger millet panicle (Eleusine coracana (L.) Gaertn) was created. The titer of obtained cDNA library comprised 3.01 x 10(5) CFU/ml in avarage. In average the length of cDNA insertion consisted about 1070 base pairs, the effectivity of cDNA fragment insertions--99.5%. The selective sequencing of cDNA clones from created library was performed. The sequences of cDNA clones were identified with usage of BLAST-search. The results of cDNA library analysis and selective sequencing represents prove good functionality and full length character of inserted cDNA clones. Obtained cDNA library from meristematic tissue of finger millet panicle represents good and valuable source for isolation and identification of key genes regulating metabolism and meristematic development and for mining of new molecular markers to conduct out high quality genetic investigations and molecular breeding as well.

  19. Preparation of fluorescent-dye-labeled cDNA from RNA for microarray hybridization.

    Science.gov (United States)

    Ares, Manuel

    2014-01-01

    This protocol describes how to prepare fluorescently labeled cDNA for hybridization to microarrays. It consists of two steps: first, a mixture of anchored oligo(dT) and random hexamers is used to prime amine-modified cDNA synthesis by reverse transcriptase using a modified deoxynucleotide with a reactive amine group (aminoallyl-dUTP) and an RNA sample as a template. Second, the cDNA is purified and exchanged into bicarbonate buffer so that the amine groups in the cDNA react with the dye N-hydroxysuccinimide (NHS) esters, covalently joining the dye to the cDNA. The dye-coupled cDNA is purified again, and the amount of dye incorporated per microgram of cDNA is determined.

  20. Potential use of eucalyptus biodiesel in compressed ignition engine

    Directory of Open Access Journals (Sweden)

    Puneet Verma

    2016-03-01

    Full Text Available The increased population has resulted in extra use of conventional sources of fuels due to which there is risk of extinction of fossil fuels’ resources especially petroleum diesel. Biodiesel is emerging as an excellent alternative choice across the world as a direct replacement for diesel fuel in vehicle engines. Biodiesel offers a great choice. It is mainly derived from vegetable oils, animal fats and algae. Hence in this paper effort has been made to find out feasibility of biodiesel obtained from eucalyptus oil and its impact on diesel engine. Higher viscosity is a major issue while using vegetable oil directly in engine which can be removed by converting it into biodiesel by the process of transesterification. Various fuel properties like calorific value, flash point and cetane value of biodiesel and biodiesel–diesel blends of different proportions were evaluated and found to be comparable with petroleum diesel. The result of investigation shows that Brake Specific Fuel Consumption (BSFC for two different samples of B10 blend of eucalyptus biodiesel is 2.34% and 2.93% lower than that for diesel. Brake Thermal Efficiency (BTE for B10 blends was found to be 0.52% and 0.94% lower than that for diesel. Emission characteristics show that Smoke Opacity improves for both samples, smoke is found to be 64.5% and 62.5% cleaner than that of diesel. Out of all blends B10 was found to be a suitable alternative to conventional diesel fuel to control air pollution without much significant effect on engine performance. On comparing both samples, biodiesel prepared from sample A of eucalyptus oil was found to be superior in all aspects of performance and emission.

  1. ECONOMIC ROTATION OF Eucalyptus grandis PLANTATIONS FOR PULP PRODUCTION

    Directory of Open Access Journals (Sweden)

    Thais Cunha Ferreira

    2004-07-01

    Full Text Available The objectives of the research were: to determine the economic impact of several minimum diameter and length of logs in economic rotation age, economic feasibility of Eucalyptus grandis plantation for cellulose production; to determine the economic loss of cutting the stand before or after the optimal economic rotation age. A biometric model for making wood volume prognosis was developed using data of a trial of Eucalyptus grandis plantation envisaging pulp production. Eucalyptus grandis stands of 19 and 103 months old, in the spacing 3 x 2 and 3 x 3 m in site index of 30; 28; 26 and 24 m were used. Theprognosis started at the age zero, considering logs of 2.5; 2.8; 4.0 and 6.0 m of length for minimum diameter varying from 4 to 10 cm, in intervals of 2 cm. Net Present Worth (VPL was used the economic criterion, considering an infinite horizon and a cost relation including reestablishment, yearly maintenance, logging and wood transportation costs. The main conclusions were: increases in the minimum diameter and or in logs length increase the rotation age; harvesting the stands in ages different from the optimal one cause large economic loss mainly in the better sites; the economic loss is larger if the harvest is made before the optimal economic rotation than if it is make after; economic feasibility increases when the minimum diameter is smaller and when the length of the logs is shorter. Any way, before making any decision it is necessary to take into account possible technical restrictions and effect on harvest and transportation costs caused by changer in the length of logs and in the size of the minimum commercial diameter.

  2. Propagation of jarrah (Eucalyptus marginata) by organ and tissue culture

    Energy Technology Data Exchange (ETDEWEB)

    Bennett, M.J.; McComb, J.A.

    1982-01-01

    Micropropagation methods are described for the production of clonal lines from Eucalyptus marginata (jarrah) seedlings. Nodal explants from mature trees can also yield shoot cultures, but a high frequency of contamination occurs among such explants. Uncontaminated callus cultures can be produced from mature trees by culturing stamen filaments and shoots can subsequently be regenerated from this callus. The rooting percentage of shoot cultures from either nodes or stamen callus of mature trees is low compared with that from seedling explants. Considerable variation was observed between trees in the ability of stamen callus to regenerate shoots and in the frequency of rooting. (Refs. 27)

  3. Environmental aspects of eucalyptus based ethanol production and use

    International Nuclear Information System (INIS)

    González-García, Sara; Moreira, Ma. Teresa; Feijoo, Gumersindo

    2012-01-01

    A renewable biofuel economy is projected as a pathway to decrease dependence on fossil fuels as well as to reduce greenhouse gases (GHG) emissions. Ethanol produced on large-scale from lignocellulosic materials is considered the automotive fuel with the highest potential. In this paper, a life cycle assessment (LCA) study was developed to evaluate the environmental implications of the production of ethanol from a fast-growing short rotation crop (SRC): eucalyptus as well as its use in a flexi-fuel vehicle (FFV). The aim of the analysis was to assess the environmental performance of three ethanol based formulations: E10, E85 and E100, in comparison with conventional gasoline. The standard framework of LCA from International Standards Organization was followed and the system boundaries included the cultivation of the eucalyptus biomass, the processing to ethanol conversion, the blending with gasoline (when required) and the final use of fuels. The environmental results show reductions in all impact categories under assessment when shifting to ethanol based fuels, excluding photochemical oxidant formation, eutrophication as well as terrestrial and marine ecotoxicity which were considerably influenced by upstream activities related to ethanol manufacture. The LCA study remarked those stages where the researchers and technicians need to work to improve the environmental performance. Special attention must be paid on ethanol production related activities, such as on-site energy generation and distillation, as well as forest activities oriented to the biomass production. The use of forest machinery with higher efficiency levels, reduction of fertilizers dose and the control of diffuse emissions from the conversion plant would improve the environmental profile. -- Highlights: ► The identification of the environmental implications of the production and use of eucalyptus based ethanol was carried out. ► Eucalyptus is a Spanish common and abundant fast-growing short

  4. Properties of Eucalyptus benthamii wood for energy production

    Directory of Open Access Journals (Sweden)

    Dimas Agostinho Silva

    2015-12-01

    Full Text Available The objective of this study was to evaluate the energy potential of Eucalyptus benthamii Maiden et Cambage wood. The samples were collected in the municipality of Cerro Negro, Santa Catarina State, Brazil. Samples were collected from 5 trees at 0%, 25%, 50%, 75% and 100% of commercial height. It was determined basic density, high calorific value, elemental composition, immediate chemical analysis, lower calorific value, energy density, carbon storage and energy production. The physical and chemical variables studied and energy potential of wood did not present differences along the stem.

  5. Les plantations d' Eucalyptus au Sahel: distribution, importance ...

    African Journals Online (AJOL)

    Grâce à leur plasticité et leur rusticité, les espèces du genre Eucalyptus sont parmi les essences forestières les plus utilisées pour le reboisement en vue de lutter contre la désertification au Sahel. Toutefois, l'expansion rapide de ces espèces originaires d'Australie dans le Sahel, est surtout liée au succès économique de ...

  6. THE PHYSICAL PROPERTIES OF HEARTWOOD AND SAPWOOD OF EUCALYPTUS GRANDIS

    Directory of Open Access Journals (Sweden)

    Bekir Cihad BAL

    2012-12-01

    Full Text Available In this study, some of the physical propertiesof heartwood and sapwood of Eucalyptus grandisgrown in Karabucak, Turkey were determined. Thephysical properties determined were air-drieddensity, oven-dried density, basic density, shrinkage,swelling, fiber saturation point, and maximummoisture content. According to the test results, thephysical properties of the heartwood samplesdiffered from those of the sapwood samples due tothe presence of high proportion of juvenile wood inthe heartwood. It can be said that the shrinkage andswelling percentages were better for heartwood thansapwood. Air-dried density, oven-dried density, andbasic density of sapwood were higher than those ofheartwood.

  7. Removal of chromium (vi) by using eucalyptus bark (biosorption)

    International Nuclear Information System (INIS)

    Khatoon, S.; Anwar, J.; Fatima, H.B.

    2009-01-01

    Adsorption of Chromium (VI) on the Eucalyptus bark has been studied with variation in parameters. Different parameters like particle size of adsorbent, concentration of adsorbate, amount of adsorbent, stirring speed, time, temperature and pH were studied. The adsorption has been carried out in batch process. The adsorption capacity increases with decreasing the particle size of adsorbent. The optimum conditions for the maximum adsorption are attained with 2.0 g of adsorbent, 40 ppm metal ion concentration, at room temperature (10 degree C), with 90 min contact time, with 300 rpm agitation speed and at pH 2. (author)

  8. Cloning and analysis of the mouse Fanconi anemia group a cDNA and an overlapping penta zinc finger cDNA

    NARCIS (Netherlands)

    Wong, JCY; Alon, N; Norga, K; Kruyt, FAE; Youssoufian, H; Buchwald, M

    2000-01-01

    Despite the cloning of four disease-associated genes for Fanconi anemia (FA), the molecular pathogenesis of FA remains largely unknown. To study FA complementation group A using the mouse as a mode I system, we cloned and characterized the mouse homolog of the human FANCA cDNA, The mouse cDNA

  9. Preparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand formation with subtractively enriched cDNA fragments

    NARCIS (Netherlands)

    Hakvoort, T. B.; Spijkers, J. A.; Vermeulen, J. L.; Lamers, W. H.

    1996-01-01

    We have developed a fast and general method to obtain an enriched, full-length cDNA expression library with subtractively enriched cDNA fragments. The procedure relies on RecA-mediated triple-helix formation of single-stranded cDNA fragments with a double-stranded cDNA plasmid library. The complexes

  10. Occurrence of proscopiidae in Eucalyptus spp.: analysis of the infestations and spatial distribution

    Directory of Open Access Journals (Sweden)

    Alexandre dos Santos

    2014-12-01

    Full Text Available The objective of this research was to report the occurrence of proscopiidae and to quantify its infestation and spatial distribution in one experimental plantation of eucalypt clones. The study was conducted in an experimental field with eucalypt hybrids of Eucalyptus urophylla x Eucalyptus grandis (clones GG100, Eucalyptus urophylla x Eucalyptus grandis (H13 and of Eucalyptus urophylla x Eucalyptus camaldulensis (VM01. The plantation was established in three continuous blocks with 512 plants with 3 m x 2 m, in Cáceres municipality, Mato Grosso State, Brazil. Infestation of proscopiidae insects was detected two months after planting . The insects were collected for identification. The attacked trees were geo-referenced and the injuries were quantified. The spatial pattern of proscopiidae he attack was determinated using the location information of the damaged plants, which have been submitted to a quadrats analyses using Morisita and binomial dispersion indexes. All the collected insects have been identified as Tetanorhynchus smithi Rehn, 1904 (Orthoptera, Proscopiidae. The infestation differed significantly among the three eucalypts clones and aggregated spatial pattern of attack was observed. This is the first report of this species of proscopiidae in eucalyptus in Mato Grosso State.

  11. Antimicrobial, Physicochemical, Mechanical, and Barrier Properties of Tapioca Starch Films Incorporated with Eucalyptus Extract

    Directory of Open Access Journals (Sweden)

    M. Rojhan

    2013-11-01

    Full Text Available Starch is found in abundance in nature and it is one of the raw materials used for food packagingbecause of the low price, biodegradability, good mechanical and barrier properties. The recycling ability ofcoating materials was significantly increased by using edible films and coating in comparison to traditionalpackaging and it could be an alternative for synthetic films. In this research, the effect of eucalyptus extract(Aqueous Extract was investigated on tapioca starch films. Tapioca starch films were prepared by castingmethod with addition of eucalyptus extract and a mixture of sorbitol/glycerol (weight ratio of 3 to 1 asplasticizers. Eucalyptus extract incorporated to the tapioca starch films were dried at different concentrations(0, 15, 25, and 35 of total solid under controlled conditions. Physicochemical properties such as waterabsorption capacity (WAC, water vapor permeability (WVP and mechanical properties of the films wereevaluated. Results showed that by increasing the concentration of eucalyptus extract, tensile strength wasincreased from 20.60 to 15.68 (MPa, also elongation was increased from 19.31 to 23.57 (% at break andYoung’s modulus was decreased from 800.31 to 500.32 (MPa. Also incorporation of eucalyptus extract in thestructure of biopolymer increased permeability of water vapor of starch films. Tapioca starch filmsincorporated with eucalyptus extract exhibited excellent antimicrobial activity against E. Coli. In summary,eucalyptus extract improves functional properties of tapioca starch films and this types of films can be used infood packaging.

  12. Estimations of evapotranspiration in an age sequence of Eucalyptus plantations in subtropical China.

    Directory of Open Access Journals (Sweden)

    Wenfei Liu

    Full Text Available Eucalyptus species are widely planted for reforestation in subtropical China. However, the effects of Eucalyptus plantations on the regional water use remain poorly understood. In an age sequence of 2-, 4- and 6-year-old Eucalyptus plantations, the tree water use and soil evaporation were examined by linking model estimations and field observations. Results showed that annual evapotranspiration of each age sequence Eucalyptus plantations was 876.7, 944.1 and 1000.7 mm, respectively, accounting for 49.81%, 53.64% and 56.86% of the annual rainfall. In addition, annual soil evaporations of 2-, 4- and 6-year-old were 318.6, 336.1, and 248.7 mm of the respective Eucalyptus plantations. Our results demonstrated that Eucalyptus plantations would potentially reduce water availability due to high evapotranspiration in subtropical regions. Sustainable management strategies should be implemented to reduce water consumption in Eucalyptus plantations in the context of future climate change scenarios such as drought and warming.

  13. Grouping eucalyptus species in kraft pulp process for cost reduction

    Directory of Open Access Journals (Sweden)

    Apiwan Pichayadecha

    2014-12-01

    Full Text Available The objective of this research is to study the level of the important factors that can decrease total cost of pulp production. First of all, experts and experienced users identify the factors that affect the total production cost by applying the principle of 4M 1E cause and effect diagram. Then the primary factors were chosen based on 80% of their significance and tested by hypothesis for two population means. It was found that at the 95% confidence level the significant factors that have effects on the total production cost are amount of Effective alkali in white liquor and Kappa number. However, the proportion of easy delignification according to Eucalyptus species is considered as a significant factor based on various studies. Box-Behnken experiment is designed with respect to 3 mentioned factors and 3 levels of each factor. The response surface method (RSM is employed to determine the non-linear relation between the total cost as the response and the proportion of easy delignification, amount of Effective alkali in white liquor and Kappa number. To minimize the total cost, the optimal values of each factor are 75% of easy delignification, 112 grams per liter of Effective alkali in white liquor and 13.5 of kappa number. Under this optimal condition, the average total cost per ton of Eucalyptus is 13,393.91 Baht which is significantly less than the total cost of 15,517.06 Baht per ton before improvement.

  14. ECONOMIC EVALUATION OF Eucalyptus grandis PLANTATION FOR CELLULOSE PRODUCTION

    Directory of Open Access Journals (Sweden)

    Antonio Donizette de Oliveira

    2008-03-01

    Full Text Available The aims of this research were: to analyze the economic feasibility of planting eucalyptus for producing wood pulp,considering various site index and two spacings; to analyze the economic effects regarding the profitability of the forest activity indifferent distances from the industry and changes on discount rate, wood price, transportation costs, minimum profitable diameter oflogs and the length of the logs. A biometric model for making wood volume prognosis was developed, using data of a trial ofEucalyptus grandis stands 19 and 103 months old. The prognosis started at the age zero, considering logs of 2.5 and 6.0 m of lenghtand the minimum diameter varying from 4 to 10 cm, in intervals of 2 cm. Net Present Worth (NPW was used as the economic decisioncriterium, considering an infinite horizon. The main conclusions were: reducing the minimum profitable diameter and the length ofthe logs are good strategies to increase wood utilization and profit; plantations located in less productive lands are economicallyunfeasible; the cost of transportation has significant effect on the profitability of the forest activity and must be analyzed carefully atthe moment of defining the location of new plantations; small variations on wood sales price may cause big alterations on theprofitability of the forest activity, suggesting that the improvement of the wood quality together with other decisions that may increasewood price are alternatives that may render the plantations in less productive areas profitable.

  15. Thinning regimes and initial spacing for Eucalyptus plantations in Brazil.

    Science.gov (United States)

    Ferraz Filho, Antonio C; Mola-Yudego, Blas; González-Olabarria, José R; Scolforo, José Roberto S

    2018-01-01

    This study focuses on the effects of different thinning regimes on clonal Eucalyptus plantations growth. Four different trials, planted in 1999 and located in Bahia and Espírito Santo States, were used. Aside from thinning, initial planting density, and post thinning fertilization application were also evaluated. Before canopy closure, and therefore before excessive competition between trees took place, it was found that stands planted under low densities (667 trees per hectare) presented a lower mortality proportion when compared to stand planted under higher densities (1111 trees per hectare). However, diameter growth prior to thinning operations was not statistically different between these two densities, presenting an overall mean of 4.9 cm/year. After canopy closure and the application of the thinning treatments, it was found that thinning regimes beginning early in the life of the stand and leaving a low number of residual trees presented the highest diameter and height growth. Unthinned treatments and thinning regimes late in the life of the stand (after 5.5 years), leaving a large number of residual trees presented the highest values of basal area production. The choice of the best thinning regime for Eucalyptus clonal material will vary according to the plantation objective.

  16. Predicted stand volume for Eucalyptus plantations by spatial analysis

    Science.gov (United States)

    Latifah, Siti; Teodoro, RV; Myrna, GC; Nathaniel, CB; Leonardo, M. F.

    2018-03-01

    The main objective of the present study was to assess nonlinear models generated by integrating the stand volume growth rate to estimate the growth and yield of Eucalyptus. The primary data was done for point of interest (POI) of permanent sample plots (PSPs) and inventory sample plots, in Aek Nauli sector, Simalungun regency,North Sumatera Province,Indonesia. from December 2008- March 2009. Today,the demand for forestry information has continued to grow over recent years. Because many forest managers and decision makers face complex decisions, reliable information has become the necessity. In the assessment of natural resources including plantation forests have been widely used geospatial technology.The yield of Eucalyptus plantations represented by merchantable volume as dependent variable while factors affecting yield namely stands variables and the geographic variables as independent variables. The majority of the areas in the study site has stand volume class 0 - 50 m3/ha with 16.59 ha or 65.85 % of the total study site.

  17. ANALYSIS OF EUCALYPTUS GLUED-LAMINATED TIMBER PORTICOS STRUCTURAL PERFORMANCE

    Directory of Open Access Journals (Sweden)

    Sandra Maria Ferreira Couri Petrauski

    Full Text Available ABSTRACT This study evaluated the structural behavior of porticos made from eucalyptus glued boards, using wood of Eucalyptus sp and resorcinol formaldehyde adhesive. Three units, in real scale, of tri-articulated straight porticos, with a 5 meter porthole and a 26º inclination, capable to support tiles covering placement were designed, constructed and subjected to load testing, until rupture. The amount of adhesive used in the construction of the porticos was 250 g/m2 and the bonding pressure of 1.3 MPa. The Hankinson model was employed as an estimator of the glued joints strength, under different angles between the fibers. The average value for the last resistance of the structures was 4.63 times the design load, according to the criteria established by the ABNT, 1997. The structures showed satisfactory mechanical performance and deformations lower than the ones allowed by the standard. It was concluded there is technical feasibility to manufacture porticos fully bonded with small thickness veneers.

  18. ALTERNATIVES TO IMPROVE HYBRIDIZATION EFFICIENCY IN Eucalyptus BREEDING PROGRAMS

    Directory of Open Access Journals (Sweden)

    Roselaine Cristina Pereira

    2002-01-01

    Full Text Available Simple and quick hybridization procedures and ways to keep pollen grains viable for long periods are sought in plant breeding programs to provide greater work flexibility. The presentstudy was carried out to assess the efficiency of pollinations made shortly after flower emasculationand the viability of stored pollen from Eucalyptus camaldulensis and Eucalyptus urophylla clones cultivated in Northwestern Minas Gerais State. Controlled pollinations were carried out at zero, one,three, five and seven days after emasculation. Hybridization efficiency was assessed by thepercentage of viable fruits, number of seeds produced per fruit, percentage of viable seeds and also bycytological observation of the pollen development along the style. Flower buds from clones of the twospecies were collected close to anthesis to assess the viability of pollen grain storage. Pollen was thencollected and stored in a freezer (-18oC for 1, 2 and 3 months. Pollen assessed was carried out by invitro and in vivo germination tests. The efficiency of the pollinations varied with their delay and alsobetween species. The greatest pollination efficiency was obtained when they were carried out on thethird and fifth day after emasculation, but those performed simultaneously with emasculationproduced enough seeds to allow this practice in breeding programs. The decrease in pollen viabilitywith storage was not sufficiently significant to preclude the use of this procedure in artificialhybridization.

  19. Hardening of eucalyptus seedlings via salicylic acid application

    Directory of Open Access Journals (Sweden)

    Eduardo Henrique Lima Mazzuchelli

    2014-09-01

    Full Text Available The agricultural and forest productivity suffer restrictions imposed by water stress, high temperature and high solar radiation. This study aimed to evaluate the capacity of stress attenuation and growth promotion of salicylic acid (SA application in eucalyptus (E. urophylla x E. grandis hybrid seedlings under water stress. A completely randomized design, in a 3x4 factorial scheme (three water treatments: constant irrigation with daily replacement of 40% (CI40% or 100% (CI100% of evapotranspirated water, and temporary irrigation suspension with replacement of only 40% of evapotranspirated water (S40%; and four SA concentrations: 0 mg L-1, 100 mg L-1, 200 mg L-1 and 300 mg L-1, was used. Plant photosynthetic parameters and biometric features were evaluated. The stomatal limitation was higher in plants under S40% irrigation, however, the SA application reverted this result, allowing the maintenance of the photosynthetic potential. There was interaction between irrigation regimes and SA doses for number of leaves, leaf area/number of leaves ratio and shoot and root dry mass. It was concluded that the application of 200 mg L -1 of SA positively affected the growth of eucalyptus seedlings under water stress, being considered an auxiliary management technique to their hardening process.

  20. cDNA sequences of two apolipoproteins from lamprey

    International Nuclear Information System (INIS)

    Pontes, M.; Xu, X.; Graham, D.; Riley, M.; Doolittle, R.F.

    1987-01-01

    The messages for two small but abundant apolipoproteins found in lamprey blood plasma were cloned with the aid of oligonucleotide probes based on amino-terminal sequences. In both cases, numerous clones were identified in a lamprey liver cDNA library, consistent with the great abundance of these proteins in lamprey blood. One of the cDNAs (LAL1) has a coding region of 105 amino acids that corresponds to a 21-residue signal peptide, a putative 8-residue propeptide, and the 76-residue mature protein found in blood. The other cDNA (LAL2) codes for a total of 191 residues, the first 23 of which constitute a signal peptide. The two proteins, which occur in the high-density lipoprotein fraction of ultracentrifuged plasma, have amino acid compositions similar to those of apolipoproteins found in mammalian blood; computer analysis indicates that the sequences are largely helix-permissive. When the sequences were searched against an amino acid sequence data base, rat apolipoprotein IV was the best matching candidate in both cases. Although a reasonable alignment can be made with that sequence and LAL1, definitive assignment of the two lamprey proteins to typical mammalian classes cannot be made at this point

  1. Infectious Maize rayado fino virus from Cloned cDNA.

    Science.gov (United States)

    Edwards, Michael C; Weiland, John J; Todd, Jane; Stewart, Lucy R

    2015-06-01

    A full-length cDNA clone was produced from a U.S. isolate of Maize rayado fino virus (MRFV), the type member of the genus Marafivirus within the family Tymoviridae. Infectivity of transcripts derived from cDNA clones was demonstrated by infection of maize plants and protoplasts, as well as by transmission via the known leafhopper vectors Dalbulus maidis and Graminella nigrifrons that transmit the virus in a persistent-propagative manner. Infection of maize plants through vascular puncture inoculation of seed with transcript RNA resulted in the induction of fine stipple stripe symptoms typical of those produced by wild-type MRFV and a frequency of infection comparable with that of the wild type. Northern and Western blotting confirmed the production of MRFV-specific RNAs and proteins in infected plants and protoplasts. An unanticipated increase in subgenomic RNA synthesis over levels in infected plants was observed in protoplasts infected with either wild-type or cloned virus. A conserved cleavage site motif previously demonstrated to function in both Oat blue dwarf virus capsid protein and tymoviral nonstructural protein processing was identified near the amino terminus of the MRFV replicase polyprotein, suggesting that cleavage at this site also may occur.

  2. Automation of cDNA Synthesis and Labelling Improves Reproducibility

    Directory of Open Access Journals (Sweden)

    Daniel Klevebring

    2009-01-01

    Full Text Available Background. Several technologies, such as in-depth sequencing and microarrays, enable large-scale interrogation of genomes and transcriptomes. In this study, we asses reproducibility and throughput by moving all laboratory procedures to a robotic workstation, capable of handling superparamagnetic beads. Here, we describe a fully automated procedure for cDNA synthesis and labelling for microarrays, where the purification steps prior to and after labelling are based on precipitation of DNA on carboxylic acid-coated paramagnetic beads. Results. The fully automated procedure allows for samples arrayed on a microtiter plate to be processed in parallel without manual intervention and ensuring high reproducibility. We compare our results to a manual sample preparation procedure and, in addition, use a comprehensive reference dataset to show that the protocol described performs better than similar manual procedures. Conclusions. We demonstrate, in an automated gene expression microarray experiment, a reduced variance between replicates, resulting in an increase in the statistical power to detect differentially expressed genes, thus allowing smaller differences between samples to be identified. This protocol can with minor modifications be used to create cDNA libraries for other applications such as in-depth analysis using next-generation sequencing technologies.

  3. Qualidade da celulose kraft-antraquinona de Eucalyptus dunnii plantado em cinco espaçamentos em relação ao Eucalyptus grandis e Eucalyptus saligna.

    OpenAIRE

    Giovanni Willer Ferreira; Jorge Vieira Gonzaga; Celso Edmundo B. Foelkel; Teotônio Francisco de Assis; Edvins Ratnieks; Maria Cladis Mezzomo da Silva

    2009-01-01

    Este trabalho analisou o efeito de cinco espaçamentos de Eucalyptus dunnii (3 m x 1 m; 3 m x 1,5 m; 3 m x 2 m; 3 m x 3 m; 3 m x 4 m), para produção de celulose kraft-antraquinona, comparados com Eucalyptus grandis e Eucalyptus saligna (ambos no espaçamento 3 m x 2 m). Avaliaram-se a densidade básica e composição química da madeira original, analisando-se extrativos em diclorometano, pentosanas, lignina, solubilidade em NaOH8% e cinzas. Os cavacos foram submetidos a cozimento kraft-antraqu...

  4. Lack of association between allozyme heterozygosity and juvenile traits in Eucalyptus

    Science.gov (United States)

    Genetic variability for juvenile waits, which included basal diameter, height, biomass accumulation, and growth increment, was studied in eight provenances involving four species, Eucalyptus grandis, E. saligna, E. camaldulensis and E. urophylla, under uniform greenhouse conditions. The species diff...

  5. Isolation and molecular characterization of Cryptococcus species isolated from pigeon nests and Eucalyptus trees

    Directory of Open Access Journals (Sweden)

    Abuzar Kamari

    2017-06-01

    Conclusion: The presence of pigeons and Eucalyptus trees in the vicinity of some particular places such as rest homes and hospitals should be considered as a risk factor for the immunocompromised population.  

  6. A rapid method for screening arrayed plasmid cDNA library by PCR

    International Nuclear Information System (INIS)

    Hu Yingchun; Zhang Kaitai; Wu Dechang; Li Gang; Xiang Xiaoqiong

    1999-01-01

    Objective: To develop a PCR-based method for rapid and effective screening of arrayed plasmid cDNA library. Methods: The plasmid cDNA library was arrayed and screened by PCR with a particular set of primers. Results: Four positive clones were obtained through about one week. Conclusion: This method can be applied to screening not only normal cDNA clones, but also cDNA clones-containing small size fragments. This method offers significant advantages over traditional screening method in terms of sensitivity, specificity and efficiency

  7. Functional cloning using pFB retroviral cDNA expression libraries.

    Science.gov (United States)

    Felts, Katherine A; Chen, Keith; Zaharee, Kim; Sundar, Latha; Limjoco, Jamie; Miller, Anna; Vaillancourt, Peter

    2002-09-01

    Retroviral cDNA expression libraries allow the efficient introduction of complex cDNA libraries into virtually any mitotic cell type for screening based on gene function. The cDNA copy number per cell can be easily controlled by adjusting the multiplicity of infection, thus cell populations may be generated in which >90% of infected cells contain one to three cDNAs. We describe the isolation of two known oncogenes and one cell-surface receptor from a human Burkitt's lymphoma (Daudi) cDNA library inserted into the high-titer retroviral vector pFB.

  8. BASIC DENSITY AND RETRACTIBILITY OF WOOD CLONES OF THREE Eucalyptus SPECIES

    Directory of Open Access Journals (Sweden)

    Djeison Cesar Batista

    2010-12-01

    Full Text Available Among the planted forests that supply the national wood industry, the genus Eucalyptus has become the most important, due to its fast growth, ease of large scale planting and variability of wood use. The generation of new hybrids and clones is a reality in the national practice of silviculture, and there is great interest currently in finding genetic improvements, mainly for higher volumetric gains and resistance in rough conditions of planting, such as pest attacks, periods of drought, low soil fertility, etc. The basic density is one of the most important physical properties of wood because it relates directly to other properties, including the anisotropic shrinkage. Such properties indicate the rational use of a species in a certain wood product. The aim of this work was to determine the basic density and the anisotropic shrinkage of five wood clones for each one of the following species: Eucalyptus saligna, Eucalyptus grandis and Eucalyptus dunnii. Clone 5 of Eucalyptus saligna presented the highest basic density (0.56 g/cm³ and was the most dimensionally instable. Of all the species, there was only a direct relation among basic density, maximum volumetric shrinkage and maximum volumetric shrinkage coefficient in this clone. Considering maximum volumetric shrinkage as the criterion, clone 3 was the most dimensionally stable. Clones 2 and 3 of Eucalyptus grandis presented the least and the highest basic density, respectively, with 0.40 and 0.49 g/cm³. It was not possible to distinguish among clones 1, 3 and 4 in terms of dimensional stability, and considering maximum volumetric shrinkage coefficient as the criterion, clone 5 was the most dimensionally instable. For Eucalyptus saligna and Eucalyptus dunnii it was not possible to distinguish which clone presented the least basic density. Clone 3 of Eucalyptus dunnii presented the highest basic density (0.65 g/cm³ and considering maximum volumetric shrinkage coefficient as the criterion, it

  9. MODEL OF A PROCESS FOR DRYING Eucalyptus spp AT HIGH TEMPERATURES

    Directory of Open Access Journals (Sweden)

    P. C. C. PINHEIRO

    1998-12-01

    Full Text Available A mathematical model of a process for drying of Eucalyptus spp is presented. This model was based on fundamental heat and mass transfer equations and it was numerically solved using a segregated finite volume method. Software in the FORTRAN language was developed to solve the mathematical model. The kinetic parameters of drying for Eucalyptus spp were experimentally obtained by isothermal thermogravimetry (TG. The theoretical results generated using the mathematical model were validated by experimental data.

  10. Ansel Adams's Eucalyptus Tree, Fort Ross: Nature, Photography, and the Search for California

    OpenAIRE

    Adam Arenson

    2005-01-01

    This article considers the image of California evoked in the unusual Ansel Adams photograph Eucalyptus Tree, Fort Ross, California (1969), a Polaroid Land image of the garrison fence and an aged eucalyptus tree. Considering the participation of Russian occupation, Australian cross-pollination, Carleton Watkins's early photographs of redwoods, automotive and tourist images in the creation of this distinctive California place, the article argues that to understand Ansel Adams's work, we must no...

  11. EUCANEXT: an integrated database for the exploration of genomic and transcriptomic data from Eucalyptus species

    OpenAIRE

    Nascimento, Leandro Costa; Salazar, Marcela Mendes; Lepikson-Neto, Jorge; Camargo, Eduardo Leal Oliveira; Parreiras, Lucas Salera; Pereira, Gonçalo Amarante Guimarães; Carazzolle, Marcelo Falsarella

    2017-01-01

    Abstract Tree species of the genus Eucalyptus are the most valuable and widely planted hardwoods in the world. Given the economic importance of Eucalyptus trees, much effort has been made towards the generation of specimens with superior forestry properties that can deliver high-quality feedstocks, customized to the industrýs needs for both cellulosic (paper) and lignocellulosic biomass production. In line with these efforts, large sets of molecular data have been generated by several scienti...

  12. Wood CO2 efflux and foliar respiration for Eucalyptus in Hawaii and Brazil

    Science.gov (United States)

    Michael G. Ryan; Molly A. Cavaleri; Auro C. Almeida; Ricardo Penchel; Randy S. Senock; Jose Luiz Stape

    2009-01-01

    We measured CO2 efflux from wood for Eucalyptus in Hawaii for 7 years and compared these measurements with those on three- and four-and-a-halfyear- old Eucalyptus in Brazil. In Hawaii, CO2 efflux from wood per unit biomass declined ~10x from age two to age five, twice as much as the decline in tree growth. The CO2 efflux from wood in Brazil was 8-10· lower than that...

  13. Soil microbial community structure and function responses to successive planting of Eucalyptus.

    Science.gov (United States)

    Chen, Falin; Zheng, Hua; Zhang, Kai; Ouyang, Zhiyun; Li, Huailin; Wu, Bing; Shi, Qian

    2013-10-01

    Many studies have shown soil degradation after the conversion of native forests to exotic Eucalyptus plantations. However, few studies have investigated the long-term impacts of short-rotation forestry practices on soil microorganisms. The impacts of Eucalyptus successive rotations on soil microbial communities were evaluated by comparing phospholipid fatty acid (PLFA) abundances, compositions, and enzyme activities of native Pinus massoniana plantations and adjacent 1st, 2nd, 3rd, 4th generation Eucalyptus plantations. The conversion from P. massoniana to Eucalyptus plantations significantly decreased soil microbial community size and enzyme activities, and increased microbial physiological stress. However, the PLFA abundances formed "u" shaped quadratic functions with Eucalyptus plantation age. Alternatively, physiological stress biomarkers, the ratios of monounsaturated to saturated fatty acid and Gram+ to Gram- bacteria, formed "n"' shaped quadratic functions, and the ratio of cy17:0 to 16:1omega7c decreased with plantation age. The activities of phenol oxidase, peroxidase, and acid phosphatase increased with Eucalyptus plantation age, while the cellobiohydrolase activity formed "u" shaped quadratic functions. Soil N:P, alkaline hydrolytic nitrogen, soil organic carbon, and understory cover largely explained the variation in PLFA profiles while soil N:P, alkaline hydrolytic nitrogen, and understory cover explained most of the variability in enzyme activity. In conclusion, soil microbial structure and function under Eucalyptus plantations were strongly impacted by plantation age. Most of the changes could be explained by altered soil resource availability and understory cover associated with successive planting of Eucalyptus. Our results highlight the importance of plantation age for assessing the impacts of plantation conversion as well as the importance of reducing disturbance for plantation management.

  14. Study on the Modification of Bleached Eucalyptus Kraft Pulp Using Birch Xylan

    Science.gov (United States)

    Wenjia Han; Chuanshan Zhao; Thomas Elder; Rendang Yang; Dongho Kim; Yunqiao Pu; Jeffery Hsieh; Arthur J. Ragauskas

    2012-01-01

    In this study, birch xylan was deposited onto elementally chlorine free (ECF) bleached eucalyptus kraft pulp, and the corresponding changes in physical properties were determined. An aqueous 5% birch xylan solution at pH 9 was added to 5 wt% slurry of bleached kraft eucalyptus fibers, with stirring at 70 C for 15 min after which the pH was adjusted to 5–6. The xylan...

  15. Expression analysis of a ''Cucurbita'' cDNA encoding endonuclease

    International Nuclear Information System (INIS)

    Szopa, J.

    1995-01-01

    The nuclear matrices of plant cell nuclei display intrinsic nuclease activity which consists in nicking supercoiled DNA. A cDNA encoding a 32 kDa endonuclease has been cloned and sequenced. The nucleotide and deduced amino-acid sequences show high homology to known 14-3-3-protein sequences from other sources. The amino-acid sequence shows agreement with consensus sequences for potential phosphorylation by protein kinase A and C and for calcium, lipid and membrane-binding sites. The nucleotide-binding site is also present within the conserved part of the sequence. By Northern blot analysis, the differential expression of the corresponding mRNA was detected; it was the strongest in sink tissues. The endonuclease activity found on DNA-polyacrylamide gel electrophoresis coincided with mRNA content and was the highest in tuber. (author). 22 refs, 6 figs

  16. Basic density and pulp yield relationship with some chemical parameters in eucalyptus trees Densidade e rendimento em polpa celulósica e sua relação com alguns parâmetros químicos no eucalipto

    Directory of Open Access Journals (Sweden)

    Ana Maria Loureiro da Seca

    2006-12-01

    Full Text Available The objective of this work was to evaluate the use of basic density and pulp yield correlations with some chemical parameters, in order to differentiate an homogeneous eucalyptus tree population, in terms of its potential for pulp production or some other technological applications. Basic density and kraft pulp yield were determined for 120 Eucalyptus globulus trees, and the values were plotted as frequency distributions. Homogenized samples from the first and fourth density quartiles and first and fourth yield quartiles were submitted to total phenols, total sugars and methoxyl group analysis. Syringyl/guaiacyl (S/G and syringaldehyde/vanillin (S/V ratios were determined on the kraft lignins from wood of the same quartiles. The results show the similarity between samples from high density and low yield quartiles, both with lower S/G (3.88-4.12 and S/V (3.99-4.09 ratios and higher total phenols (13.3-14.3 g gallic acid kg-1 . Woods from the high yield quartile are statistically distinguished from all the others because of their higher S/G (5.15 and S/V (4.98 ratios and lower total phenols (8.7 g gallic acid kg-1 . Methoxyl group and total sugars parameters are more adequate to distinguish wood samples with lower density.O objetivo deste trabalho foi avaliar a aplicabilidade de correlações entre rendimento em polpa e densidade básica com alguns parâmetros químicos, para diferenciar uma população homogênea de árvores de eucalipto, em termos de capacidade de produção de polpa celulósica ou de outras aplicações. Determinou-se a densidade básica e o rendimento em polpa celulósica em 120 árvores de Eucalyptus globulus, e os resultados foram representados como distribuição de freqüências. Na madeira das árvores dos primeiro e quarto quartis de densidade e de rendimento, determinaram-se os teores de fenóis totais, açúcares totais e grupos metoxílicos. Nas respectivas ligninas kraft determinaram-se as razões seringilo

  17. Eucalyptus tolerance mechanisms to lanthanum and cerium: subcellular distribution, antioxidant system and thiol pools.

    Science.gov (United States)

    Shen, Yichang; Zhang, Shirong; Li, Sen; Xu, Xiaoxun; Jia, Yongxia; Gong, Guoshu

    2014-12-01

    Guanglin 9 (Eucalyptus grandis × Eucalyptus urophlla) and Eucalyptus grandis 5 are two eucalyptus species which have been found to grow normally in soils contaminated with lanthanum and cerium, but the tolerance mechanisms are not clear yet. In this study, a pot experiment was conducted to investigate the tolerance mechanisms of the eucalyptus to lanthanum and cerium. Cell walls stored 45.40-63.44% of the metals under lanthanum or cerium stress. Peroxidase and catalase activities enhanced with increasing soil La or Ce concentrations up to 200 mg kg(-1), while there were no obvious changes in glutathione and ascorbate concentrations. Non-protein thiols concentrations increased with increasing treatment levels up to 200 mg kg(-1), and then decreased. Phytochelatins concentrations continued to increase under La or Ce stress. Therefore, the two eucalyptus species are La and Ce tolerant plants, and the tolerance mechanisms include cell wall deposition, antioxidant system response, and thiol compound synthesis. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. Eucalyptus essential oil as a natural food preservative: in vivo and in vitro antiyeast potential.

    Science.gov (United States)

    Tyagi, Amit Kumar; Bukvicki, Danka; Gottardi, Davide; Tabanelli, Giulia; Montanari, Chiara; Malik, Anushree; Guerzoni, Maria Elisabetta

    2014-01-01

    In this study, the application of eucalyptus essential oil/vapour as beverages preservative is reported. The chemical composition of eucalyptus oil was determined by gas chromatography-mass spectrometry (GC-MS) and solid phase microextraction GC-MS (SPME/GC-MS) analyses. GC-MS revealed that the major constituents were 1,8-cineole (80.5%), limonene (6.5%), α-pinene (5%), and γ-terpinene (2.9%) while SPME/GC-MS showed a relative reduction of 1,8-cineole (63.9%) and an increase of limonene (13.8%), α-pinene (8.87%), and γ-terpinene (3.98%). Antimicrobial potential of essential oil was initially determined in vitro against 8 different food spoilage yeasts by disc diffusion, disc volatilization, and microdilution method. The activity of eucalyptus vapours was significantly higher than the eucalyptus oil. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) varied from 0.56 to 4.50 mg/mL and from 1.13 to 9 mg/mL, respectively. Subsequently, the combined efficacy of essential oil and thermal treatment were used to evaluate the preservation of a mixed fruit juice in a time-dependent manner. These results suggest eucalyptus oil as a potent inhibitor of food spoilage yeasts not only in vitro but also in a real food system. Currently, this is the first report that uses eucalyptus essential oil for fruit juice preservation against food spoiling yeast.

  19. USE OF TREND IMPACT ANALYSIS AFFECTS PROJECTIONS OF EUCALYPTUS CULTIVATION IN BRAZIL

    Directory of Open Access Journals (Sweden)

    Samir Lotfi

    2009-10-01

    Full Text Available Eucalyptus forestry is an important source of competitive advantage for Brazil and, since eucalyptus is a basic raw material for industrial segments that are undergoing great expansion, monitoring the growth rate of cultivated area is increasingly relevant.This study resorted to Trend Impact Analysis (TIA to forecast the planting of eucalyptus in Brazil’s reforested areas, adjusting the linear regression of historical data in the light of three events that were under way or that will probably influence the trend: timber funds, the world financial crisis and Biomass to Liquid (BTL technology.The results allow one to infer that, in the short term, eucalyptus cultivation will expand at a rate similar to that of the linear curve, adversely affected by the world crisis and positively affected by timber funds.By 2016, however, the expansion of eucalyptus plantations is expected, largely because of the commercial scale of BTL technology.Key words: Trend Impact Analysis. Eucalyptus. Future Studies.

  20. Production cost of biomasses from eucalyptus and elefant grass for energy

    Directory of Open Access Journals (Sweden)

    Laurent Marie Roger Quéno

    2011-09-01

    Full Text Available This work established the unit energy cost generated from biomass of eucalyptus (Eucalyptus sp. and elephant grass (Pennisetum sp. and applied a sensitivity analysis to verify the influences of factors such as the silviculture of eucalyptus, production volume of each species, the cost of land and the interest rate. It was shown that the treatment of eucalyptus in very short rotation of 2 years with reform of stand every 6 years has a average cost of production higher than the traditional treatment of short rotation of 6 years with reform only at the age of 18. It was also observed that eucalyptus has a Production Cost on average of R$ 4,41 /Gj, lower than the elephant grass which is on average of R$ 5,44/Gj, which however has a higher annual capacity of dry matter production. The elephant grass has the possibility to compete with eucalyptus when a set of conditions is met: discount rate higher than or equal to 8%, High price of land, and elephant grass high volume production, greater than or equal to 35 tonnes of dry matter per hectare and year.

  1. Eucalyptus biodiesel as an alternative to diesel fuel: preparation and tests on DI diesel engine.

    Science.gov (United States)

    Tarabet, Lyes; Loubar, Khaled; Lounici, Mohand Said; Hanchi, Samir; Tazerout, Mohand

    2012-01-01

    Nowadays, the increasing oil consumption throughout the world induces crucial economical, security, and environmental problems. As a result, intensive researches are undertaken to find appropriate substitution to fossil fuels. In view of the large amount of eucalyptus trees present in arid areas, we focus in this study on the investigation of using eucalyptus biodiesel as fuel in diesel engine. Eucalyptus oil is converted by transesterification into biodiesel. Eucalyptus biodiesel characterization shows that the physicochemical properties are comparable to those of diesel fuel. In the second phase, a single cylinder air-cooled, DI diesel engine was used to test neat eucalyptus biodiesel and its blends with diesel fuel in various ratios (75, 50, and 25 by v%) at several engine loads. The engine combustion parameters such as peak pressure, rate of pressure rise, and heat release rate are determined. Performances and exhaust emissions are also evaluated at all operating conditions. Results show that neat eucalyptus biodiesel and its blends present significant improvements of carbon monoxide, unburned hydrocarbon, and particulates emissions especially at high loads with equivalent performances to those of diesel fuel. However, the NOx emissions are slightly increased when the biodiesel content is increased in the blend.

  2. Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning

    International Nuclear Information System (INIS)

    Han, J.H.; Stratowa, C.; Rutter, W.J.

    1987-01-01

    The authors have cloned a full-length putative rat pancreatic lysophospholipase cDNA by an improved mRNA isolation method and cDNA cloning strategy using [ 32 P]-labelled nucleotides. These new methods allow the construction of a cDNA library from the adult rat pancreas in which the majority of recombinant clones contained complete sequences for the corresponding mRNAs. A previously recognized but unidentified long and relatively rare cDNA clone containing the entire sequence from the cap site at the 5' end to the poly(A) tail at the 3' end of the mRNA was isolated by single-step screening of the library. The size, amino acid composition, and the activity of the protein expressed in heterologous cells strongly suggest this mRNA codes for lysophospholipase

  3. Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla Organogênese e transformação genética transiente do híbrido Eucalyptus grandis × Eucalyptus urophylla

    Directory of Open Access Journals (Sweden)

    Giovana Bomfim de Alcantara

    2011-04-01

    Full Text Available The hybrid Eucalyptus grandis x Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E. urophylla was studied in order to verify the effect of several factors: subculture duration on multiplication medium, type of explants, entire and half leaves: basal and apical portions, and duration of the culture on a regeneration medium. Differences in organogenic capacity of the two clones tested were observed. The explant most recommended for organogenesis is the basal section of the leaf collected from shoot clusters subcultured every 17 days. Moreover, the leaf explants must be transferred to a fresh bud induction medium every five days. This study also aimed at evaluating factors affecting the genetic transformation of leaf explants with the uidA gene, via co-cultivation with Agrobacterium tumefaciens, such as the pre-culture of the explants on a specific medium, the duration of their co-culture with the bacteria and the addition of acetosyringone to the culture media. The best conditions for the expression of the uidA gene were two days of pre-culture of the leaf tissues, three days of co-culture with the bacteria and the addition of acetosyringone in pre- and co-culture media.O híbrido Eucalyptus grandis x Eucalyptus urophylla apresenta alta produtividade e potencial para indústrias de papel, celulose e fibras. A organogênese de explantes foliares de dois clones de E. grandis × E. urophylla foi estudada para verificar fatores como tempo de repicagem das plântulas matrizes em meio de multiplicação; tipo de explantes, folhas inteiras e de meias-folhas (porções basais e apicais e dos dias que os explantes foliares permaneceram em meio de regeneração. Foram observadas diferenças na capacidade organogênica dos dois clones testados. A parte basal das folhas, coletadas de brotações repicadas a cada 17

  4. QUALITY OF SAWN TIMBER DRYING OF Eucalyptus dunnii

    Directory of Open Access Journals (Sweden)

    Elias Taylor Durgante Severo

    2009-09-01

    Full Text Available In the present study, the behaviour of Eucalyptus dunnii wood drying in a conventional pilot kiln was evaluated, using three drying schedules. Each drying schedule had an initial vaporization (presteaming of 3 hours after one hour of initial warming and an intermediate vaporization of 5 hours for the collapse recovery when the wood was at 17% of humidity. The results show that among the used drying schedule only the schedule regarded as the mildest presented a good result in terms of dry wood quality. Among the discovered defects, there were 37,5% of collapse, 35% of cupping and 10% of split. Hoviever, though from this total only 15% of the collapse and cupping were regarded as strong defects.

  5. Dynamics of adventitious rooting in mini-cuttings of Eucalyptus benthamii x Eucalyptus dunnii=Dinamica de enraizamento adventício em miniestacas de Eucalyptus benthamii x Eucalyptus dunnii

    Directory of Open Access Journals (Sweden)

    Antônio Natal Gonçalves

    2012-04-01

    Full Text Available It is possible to determine the optimum time for permanence of vegetative propagules (mini-cuttings inside a greenhouse for rooting, and this value can be used to optimize the structure of the nursery. The aim of this study was to determine the dynamics of adventitious rooting in mini-cuttings of three clones of Eucalyptus benthamii x Eucalyptus dunnii. Sprouts of H12, H19 and H20 clones were collected from mini-stumps that were planted in gutters containing sand and grown in a semi-hydroponic system. The basal region of the mini-cuttings was immersed in 2,000 mg L-1 indole-3-butyric acid (IBA solution for 10 seconds. The rooting percentage of the mini-cuttings, the total length of the root system and the rooting rate per mini-cutting were also evaluated at 0 (time of planting, 7, 14, 21, 28, 35, 42, 49 and 56 days. We used logistic and exponential regression to mathematically model the speed of rhizogenesis. The rooting percentage was best represented as a logistic model, and the total length of the root system was best represented as an exponential model. The clones had different speeds of adventitious rooting. The optimum time for permanence of the mini-cuttings inside the greenhouse for rooting was between 35 and 42 days, and varied depending on the genetic material.O tempo ideal de permanência de propágulos vegetativos (miniestacas no interior da casa de vegetação para a rizogênese é possível de ser determinado matematicamente, o que pode otimizar as instalações do viveiro. O objetivo deste estudo foi determinar a dinâmica de enraizamento de miniestacas de três clones de Eucalyptus benthamii x Eucalyptus dunnii. Brotações dos clones H19, H12 e H20 foram coletadas de minicepas plantadas em canaletão com areia e cultivadas sob sistema semi-hidropônico. A região basal da miniestaca foi imersa em solução de 2.000 mg L-1 de ácido indolbutírico (AIB por 10 segundos. A porcentagem de enraizamento de miniestacas, o comprimento

  6. Polyphenol compounds of the kino of Eucalyptus citriodora

    International Nuclear Information System (INIS)

    Freitas, Marinalva Oliveira; Lima, Mary Anne S.; Silveira, Edilberto R.

    2007-01-01

    Phytochemical analysis of the kino of Eucalyptus citriodora led to the isolation of 1-O,2-O-digaloil-6-O-trans-p-cumaroil-beta-D-glucopyranoside, 1-O-trans-p-cumaroil-6-O-cinamoil-beta-D-glucopyranoside, alpha and beta 6-O-trans-p-cumaroil-D-glucopyranoside, 7-methylaromadendrin-4'-O-6 - trans-p-cumaroil-beta-Dglucopyranoside, aromadendrin, aromadendrin-7-methyl-ether, naringenin, sakuranetin, kaempferol-7-methyl-ether and galic acid. Structural elucidation of the isolated compounds was established on the basis of spectral data, particularly by the use of 1D NMR and several 2D shift correlated NMR pulse sequences ( 1 H, 1 H-COSY, HMQC, HMBC). (author)

  7. Qualidade da secagem de madeira serrada de eucalyptus dunnii

    Directory of Open Access Journals (Sweden)

    2000-01-01

    Full Text Available In the present study, the behaviour of Eucalyptus dunnii wood drying in a conventional pilot kiln was evaluated, using three drying schedules. Each drying schedule had an initial vaporization (presteaming of 3 hours after one hour of initial warming and an intermediate vaporization of 5 hours for the collapse recovery when the wood was at 17% of humidity. The results show that among the used drying schedule only the schedule regarded as the mildest presented a good result in terms of dry wood quality. Among the discovered defects, there were 37,5% of collapse, 35% of cupping and 10% of split. Hoviever, though from this total only 15% of the collapse and cupping were regarded as strong defects.

  8. Myrtus comunis and Eucalyptus camaldulensis cytotoxicity on breast cancer cells

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    Hrubik Jelena D.

    2012-01-01

    Full Text Available In vitro cytotoxicity of methanol, ethyl acetate, n-buthanol, and water extracts of Myrtus communis L. and Eucalyptus camaldulensis Dehnh. was examined against two human breast cancer cell lines (MCF 7 and MDA-MB-231 using MTT and SRB assays. The results showed significant cytotoxic potential of examined extracts, with IC50 values ranging from 7 to 138 μg/ml for M. communis and 3-250 μg/ml for E. camaldulensis. The two plants generally expressed similar activity, and no significant difference in cell line’s sensitivity towards extracts was observed. The results indicate to M. communis and E. camaldulensis as candidates for thorough chemical analyses for identification of active compounds, and eventually for attention in the process of discovery of new natural products in the control of cancer. [Projekat Ministarstva nauke Republike Srbije, br. 173037 i br. 172058

  9. Nanofibrillated cellulose as paper additive in Eucalyptus pulps

    Directory of Open Access Journals (Sweden)

    Israel González

    2012-11-01

    Full Text Available In this work, the physical and mechanical properties of bleached Eucalyptus pulp reinforced with nanofibrillated cellulose (NFC are compared with those of traditional beaten pulp used in the making of writing/printing and offset printing papers. For this purpose, three different types of hardwood slurries were prepared: beaten pulps, unbeaten pulps reinforced with NFC, and slightly beaten pulps also reinforced with NFC. Physical and mechanical tests were performed on handsheets from these different slurries. The results showed that adding NFC to unbeaten pulps results in physical and mechanical properties similar to those in pulps used for printing/writing papers. Nevertheless, the best results were obtained in slurries previously beaten at slight conditions and subsequently reinforced with NFC. These results demonstrate that the addition of NFC allows a reduction in beating intensity without decreasing the desired mechanical properties for this specific purpose.

  10. Estabelecimento, multiplicação e alongamento in vitro de Eucalyptus benthamii Maiden & Cambage x Eucalyptus dunnii Maiden Establishment, multiplication and elongation in vitro of Eucalyptus benthamii Maiden & Cambage x Eucalyptus dunnii Maiden

    Directory of Open Access Journals (Sweden)

    Gilvano Ebling Brondani

    2009-02-01

    Full Text Available Neste trabalho foram testadas diferentes concentrações de cloro ativo (NaOCl na assepsia de explantes para o estabelecimento in vitro, bem como benzilaminopurina (BAP e ácido naftalenoacético (ANA para a multiplicação e alongamento de Eucalyptus benthamii x Eucalyptus dunnii. As minicepas fornecedoras de propágulos para introdução in vitro foram conduzidas em minijardim clonal sob sistema semi-hidropônico. Segmentos nodais dos clones H12, H19 e H20 foram desinfestados com 0,5; 1,0; 1,5; e 2,0% (v/v de cloro ativo durante 10 min e inoculados em meio de cultura MS. Na obtenção de brotações múltiplas, utilizou-se o meio de cultura ½MS suplementado com 0; 0,25; 0,50; 0,75; e 1,0 mg L-1 de BAP. Na fase de alongamento, utilizou-se o meio de cultura ½MS com 0; 0,25; 0,50; 0,75; e 1,0 mg L-1 de ANA. Não houve interação entre os fatores estudados, obtendo-se 45%, 46% e 66% de estabelecimento do clone H12, H19 e H20, respectivamente. A concentração de BAP que resultou na maior proliferação de gemas axilares para o clone H12 aos 60 dias foi estimada na faixa de 0,25 e 0,30 mg L-1. Aos 60 dias, a faixa entre 0,25 e 0,75 mg L-1 de ANA promoveu o maior número de brotações alongadas do clone H12.This work aimed to evaluate different concentrations of active chlorine (NaOCl in explant asepsis for the establishment in vitro as well as of benzylaminopurine (BAP and naphthalene acetic acid (NAA in the multiplication and elongation of Eucalyptus benthamii x Eucalyptus dunnii. Ministumps, which supply shoots for introduction in vitro, were cultivated in a clonal mini garden under semi-hydroponic system. Nodal segments of clones H12, H19 and H20 were disinfested with 0.5, 1.0, 1.5 and 2.0% (v/v of active chlorine during 10 minutes and inoculated in MS medium. In the multiplication phase, culture ½MS medium supplemented with 0, 0.25, 0.50, 0.75 and 1.0 mg L-1 of BAP was used. In the elongation phase, ½MS medium supplemented with 0, 0.25, 0

  11. Genetic transformation of Eucalyptus camaldulensis by agrobalistic method

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    Evânia Galvão Mendonça

    2013-06-01

    Full Text Available Eucalyptus stands in the setting of worldwide forestry due to its adaptability, rapid growth, production of high-quality and low cost of wood pulp fibers. The eucalyptus convetional breeding is impaired mainlly by the long life cycle making the genetic transformation systems an important tool for this purpose. However, this system requires in vitro eficient protocols for plant induction, regeneration and seletion, that allow to obtain transgenic plants from the transformed cell groups. The aim of this work was to evaluate the callus formation and to optimize the leaves and callus genetic transformation protocol by using the Agrobacterium tumefaciens system. Concerning callus formation, two different culture media were evaluated: MS medium supplemented with auxin, cytokinin (M1 and the MS medium with reduced nitrogen concentration and supplemented with auxin, cytokinin coconut water (M2. To establish the leave genetic transformation, those were exposed to agrobiolistics technique (gene gun, to tissue injury, and A. tumesfasciens EHA 105 contening the vetor pCambia 3301 (35S::GUS::NOS, for gene transference and to establish the callus transformation thoses were exposed only to A. tumefasciens. For both experiments, the influence of different infection periods was evaluated. The M2 medium provided the best values for callus sizea and fresh and dry weight. The leaves genetic transformation using the agrobiolistics technique was effective, the gus gene transient expression could be observed. No significant differences were obtained in the infection periods (4, 6 and 8 minutes. The callus genetic transformation with A. tumefaciens also promotend the gus gene transient expression on the callus co-cultiveted for 15 e 30 minutes. The transformed callus was transfered to a regeneration and selection medium and transformed plants were obtained.

  12. QUALIDADE DA SECAGEM CONVENCIONAL CONJUNTA DA MADEIRA DE CLONES DE TRÊS ESPÉCIES DE Eucalyptus sp.

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    Djeison Cesar Batista

    2015-01-01

    Full Text Available ABSTRACT The Brazilian industry commonly dries different species of eucalypt in a same load because of the difficulty of identifying the species, hybrids and clones, and also by optimizing the use of the kiln. However, this practice is not recommended in the literature. The aim of this work was to verify the quality of grouped conventional drying of Eucalyptus saligna , Eucalyptus grandis and Eucalyptus dunnii from clonal plantations. To this end, three loads were dried with the three species together in a pilot conventional dry kiln. The criteria used to analyze the quality of dried wood were final moisture content, end checks, cupping, collapse, moisture gradient and drying stresses. Eucalyptus grandis showed the best quality of drying, followed by Eucalyptus saligna , and both species have potential to be kiln dried together. Eucalyptus dunnii had the worst quality of drying, and should not be kiln dried together with Eucalyptus grandis and Eucalyptus saligna. The basic density and shrinkage were good parameters for the expected quality of the drying of eucalyptus species, in which less dense and more dimensionally stable species had better quality of drying. The drying schedule used must be modified to further drying of these species, especially regarding to uniformity and conditioning periods, which were considered insufficient.

  13. Biomass of clone of Eucalyptus grandis x urophylla for producing briquettes; Biomassa de clone de Eucalyptus grandis x Eucalyptus urophylla para producao de briquetes

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, Eder Aparecido; Oguri, Guilherme [Universidade Estadual Paulista Julio de Mesquita Filho (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas], e-mail: os_garcias@fca.unesp.br; Lancas, Kleber Pereira [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas. Dept. de Engenharia Rural; Guerra, Saulo Philipe Sebastiao [Universidade Estadual Paulista Julio de Mesquita Filho (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas. Dept. de Gestao e Tecnologia Agroindustrial

    2011-07-01

    The aim of this work was conducted to address forest biomass energy for briquette producing. In an area of dystrophic soil, seedlings of clones of Eucalyptus grandis x E. urophylla were planted in 2008, considering factors spacing and fertilization. The first dosage of fertilizer was 70 g/plant of NPK 6-30-6 and total coverage of 110 g/plant of NPK 20-0-20 with B and Zn. The spacing was 2.8x0.5 m, 2.8x1.0 m, 2.8x1.5 m, 2.8x2.0 m and 2.8x2.5 m. At 18 months, tree samples were collected to evaluate the basic density of wood (BDW), dry biomass of stem, branches and leaves. An assessment of the economic viability of each treatment was based on the sale of briquettes. BDW spacing of 2.8x1.0 m was 0.464 kg/m{sup 3}. The largest biomass of the stem occurred in 2.8x0.5 m spacing, with dosage 3, but economically unviable. The dry biomass of branches was only affected by dosage, reaching 17.68 t/ha in the third dose. Only fertilization was significant for leaf biomass. The highest income in the spacing was 2.8 x1.5 m with dosage 2. (author)

  14. Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA

    International Nuclear Information System (INIS)

    Permutt, M.A.; Koranyi, L.; Keller, K.; Lacy, P.E.; Scharp, D.W.; Mueckler, M.

    1989-01-01

    Previous studies have suggested that pancreatic islet glucose transport is mediated by a high-K m , low-affinity facilitated transporter similar to that expressed in liver. To determine the relationship between islet and liver glucose transporters, liver-type glucose-transporter cDNA clones were isolated from a human liver cDNA library. The liver-type glucose-transporter cDNA clone hybridized to mRNA transcripts of the same size in human liver and pancreatic islet RNA. A cDNA library was prepared from purified human pancreatic islet tissue and screened with human liver-type glucose-transporter cDNA. The authors isolated two overlapping cDNA clones encompassing 2600 base pairs, which encode a pancreatic islet protein identical in sequence to that of the putative liver-type glucose-transporter protein. Xenopus oocytes injected with synthetic mRNA transcribed from a full-length cDNA construct exhibited increased uptake of 2-deoxyglucose, confirming the functional identity of the clone. These cDNA clones can now be used to study regulation of expression of the gene and to assess the role of inherited defects in this gene as a candidate for inherited susceptibility to non-insulin-dependent diabetes mellitus

  15. Peptidomics combined with cDNA library unravel the diversity of centipede venom

    DEFF Research Database (Denmark)

    Rong, Mingqiang; Yang, Shilong; Wen, Bo

    2015-01-01

    of centipede venom. In the present study, we use peptidomics combined with cDNA library to uncover the diversity of centipede Scolopendra subspinipes mutilans L. Koch. 192 peptides were identified by LC-MS/MS and 79 precursors were deduced by cDNA library. Surprisingly, the signal peptides of centipede toxins...

  16. Construction of full-length cDNA library of white flower Salvia ...

    African Journals Online (AJOL)

    In order to screen and isolate secondary metabolite biosynthesis related gene, we construct a cDNA library of white flower Salvia miltiorrhiza bge. f.alba. High quality of total RNA was successfully isolated from roots of white flower S. miltiorrhiza using modified CTAB method. Double strand cDNA was cloned into pDNR-LIB ...

  17. Tolerance of Anadenanthera peregrina to Eucalyptus camaldulensis and Eucalyptus grandis essential oil as condition for mixed plantation

    Directory of Open Access Journals (Sweden)

    Neimar de Freitas Duarte

    2012-06-01

    Full Text Available With the purpose of selecting the species of woody Caatinga for mixed plantations with Eucalyptus spp., the allelophatic effects of E. camaldulensis and E. grandis essential oil were studied on the growth activities of Anadenanthera peregrina. The plants were closed in glass chambers in the presence of volatile oil of E. camaldulensis or E. grandis at the concentration of 13 nl.cm-3. The number of leaves, height and diameter at soil lever were compared before, immediately after and after 30 days. Chlorophyll a and b, carotenoids and dry mass were evaluated after the treatment application. There was no inhibitory effect of E. camaldulensis and E. grandis oils on A. peregrina. E. camaldulensis, which was more adapted to semi-arid conditions, was planted in mixture stands with two native legume species, inoculated with Rhizobium and arbuscular mycorrhizal fungi. E. camaldulensis did not inhibit native species growth after two years of cultivation.

  18. Compostos polifenólicos do kino de Eucalyptus citriodora Polyphenol compounds of the kino of Eucalyptus citriodora

    Directory of Open Access Journals (Sweden)

    Marinalva Oliveira Freitas

    2007-01-01

    Full Text Available Phytochemical analysis of the kino of Eucalyptus citriodora led to the isolation of 1-O,2-O-digaloil-6-O-trans -p-cumaroil-beta-D-glucopyranoside, 1-O-trans-p-cumaroil-6-O -cinamoil-beta-D-glucopyranoside, alpha and beta 6-O-trans-p-cumaroil-D-glucopyranoside, 7-methylaromadendrin-4'-O-6''- trans-p-cumaroil-beta-D-glucopyranoside, aromadendrin, aromadendrin-7-methyl-ether, naringenin, sakuranetin, kaempferol-7-methyl-ether and galic acid. Structural elucidation of the isolated compounds was established on the basis of spectral data, particularly by the use of 1D NMR and several 2D shift correlated NMR pulse sequences (¹H,¹H-COSY, HMQC, HMBC.

  19. Climate Modelling Shows Increased Risk to Eucalyptus sideroxylon on the Eastern Coast of Australia Compared to Eucalyptus albens

    Directory of Open Access Journals (Sweden)

    Farzin Shabani

    2017-11-01

    Full Text Available Aim: To identify the extent and direction of range shift of Eucalyptus sideroxylon and E. albens in Australia by 2050 through an ensemble forecast of four species distribution models (SDMs. Each was generated using four global climate models (GCMs, under two representative concentration pathways (RCPs. Location: Australia. Methods: We used four SDMs of (i generalized linear model, (ii MaxEnt, (iii random forest, and (iv boosted regression tree to construct SDMs for species E. sideroxylon and E. albens under four GCMs including (a MRI-CGCM3, (b MIROC5, (c HadGEM2-AO and (d CCSM4, under two RCPs of 4.5 and 6.0. Here, the true skill statistic (TSS index was used to assess the accuracy of each SDM. Results: Results showed that E. albens and E. sideroxylon will lose large areas of their current suitable range by 2050 and E. sideroxylon is projected to gain in eastern and southeastern Australia. Some areas were also projected to remain suitable for each species between now and 2050. Our modelling showed that E. sideroxylon will lose suitable habitat on the western side and will not gain any on the eastern side because this region is one the most heavily populated areas in the country, and the populated areas are moving westward. The predicted decrease in E. sideroxylon’s distribution suggests that land managers should monitor its population closely, and evaluate whether it meets criteria for a protected legal status. Main conclusions: Both Eucalyptus sideroxylon and E. albens will be negatively affected by climate change and it is projected that E. sideroxylon will be at greater risk of losing habitat than E. albens.

  20. Molecular cloning and mammalian expression of human beta 2-glycoprotein I cDNA

    DEFF Research Database (Denmark)

    Kristensen, Torsten; Schousboe, Inger; Boel, Espen

    1991-01-01

    Human β2-glycoprotein (β2gpI) cDNA was isolated from a liver cDNA library and sequenced. The cDNA encoded a 19-residue hydrophobic signal peptide followed by the mature β2gpI of 326 amino acid residues. In liver and in the hepatoma cell line HepG2 there are two mRNA species of about 1.4 and 4.3 kb......, respectively, hybridizing specifically with the β2gpI cDNA. Upon isoelectric focusing, recombinant β2gpI obtained from expression of β2gpI cDNA in baby hamster kidney cells showed the same pattern of bands as β2gpI isolated from plasma, and at least 5 polypeptides were visible...

  1. Estimativa do tempo de vaporização de toras de Eucalyptus grandis Steaming times estimates for Eucalyptus grandis logs

    Directory of Open Access Journals (Sweden)

    Fred Willians Calonego

    2006-06-01

    Full Text Available O objetivo deste estudo foi adequar o modelo geral de determinação do tempo de vaporização de toras, proposto por Steinhagen et al. (1980, para a madeira de Eucalyptus grandis. Para tanto, foram coletadas toras de 20 a The aim this study was to adjust the general model for determining log steaming time, proposed by Steinhagen et al. (1980, for Eucalyptus grandis wood. In order to do so, logs with diameter from 20 to <25, 25 to <30 and 30 to <35 cm were collected from 14 trees of Eucalyptus grandis derived from the `Horto Florestal' nursery, Manduri, São Paulo. A thermocouple was inserted into each log near its center. The logs were steamed during 20 hours at 90ºC and 100% relative humidity. A data logger recorded the temperatures during the thermal treatment. It was concluded that the Steinhagen et al. (1980 model cannot be directly used for this species in study and corrections factors are proposed for the utilization of the general model for log steaming time, developed by Steinhagen et al. (1980, for Eucalyptus grandis wood.

  2. Utilização de madeiras de Eucalyptus grandis E Eucalyptus dunnii para produção de painéis de partículas orientadas – OSB

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2008-01-01

    Full Text Available This study was developed to evaluate the feasibility of OSB manufacturing using woods of Eucalyptus grandis and Eucalyptus dunnii. Boards with nominal density of 0,70 g/cm³ and 1,0 g/cm³ were manufactured in laboratory, using 100% of wood particles from Pinus taeda, Eucalyptus grandis and Eucalyptus dunnii, and mixtures of 50% of Pinus taeda in the internal layer of the board, with 50% of Eucalyptus grandis and 50% of Eucalyptus dunnii. The boards of Eucalyptus grandis with density of 0,70 g/cm³, as standard board density, showed the values of properties compatible with the requirements of the Canadian and European Standards and also in relation of boards manufactured from Pinus taeda. The results of the mechanical properties showed an increase in the MOE and MOR in static bending with the increase in the board density, opening the possibility to use the high density OSB for applications requiring higher strength. The results of this research indicate that wood of Eucalyptus grandis can be used as alternative specie to OSB manufacturing in the Brazil.

  3. CDNA library from the Latex of Hevea brasiliensis

    Directory of Open Access Journals (Sweden)

    Wilaiwan Chotigeat

    2010-12-01

    Full Text Available Latex from Hevea brasiliensis contains 30-50% (w/w of natural rubber (cis-1,4-polyisoprene, the important rawmaterial for many rubber industries. We have constructed a cDNA library from the latex of H. brasiliensis to investigate theexpressed genes and molecular events in the latex. We analyzed 412 expressed sequence tags (ESTs. More than 90% of theEST clones showed homology to previously described sequences in public databases. Functional classification of the ESTsshowed that the largest category were proteins of unknown function (30.1%, 11.4% of ESTs encoded for rubber synthesisrelatedproteins (RS and 8.5% for defense or stress related proteins (DS. Those with no significant homology to knownsequences (NSH accounted for 8.7%, primary metabolism (PM and gene expression and RNA metabolism were 7.8% and6.6%, respectively. Other categories included, protein synthesis-related proteins (6.6%, chromatin and DNA metabolism(CDM 3.9%, energy metabolism (EM 3.4%, cellular transport (CT 3.2%, cell structure (CS 3.2%, signal transduction (ST2.2%, secondary metabolism (SM 1.7%, protein fate (PF 2.2%, and reproductive proteins (RP 0.7%.

  4. Propriedades de chapas de flocos fabricadas com adesivo de uréia-formaldeído e de taninos da casca de Eucalyptus grandis W. Hill ex Maiden ou de Eucalyptus pellita F. Muell. Properties of flakeboards made from urea-formaldehyde and bark tannins adhesives of Eucalyptus grandis or Eucalyptus pellita

    Directory of Open Access Journals (Sweden)

    Angélica de Cássia Oliveira Carneiro

    2004-10-01

    Full Text Available Os taninos foram extraídos da casca de Eucalyptus grandis e Eucalyptus pellita, com água quente, à qual se adicionaram 4,5% de sulfito de sódio, durante três horas. As temperaturas da solução foram iguais a 70 e 100 ºC para Eucalyptus grandis e Eucalyptus pellita, respectivamente. Para a produção dos adesivos e com o intuito de reduzir a sua viscosidade, os taninos foram sulfitados com sulfito de sódio e ácido acético. Formulações adesivas foram preparadas adicionando-se 0, 25, 50, 75 ou 100% de adesivos tânicos ao adesivo comercial de uréia-formaldeído. Foram fabricadas chapas de flocos de Pinus elliottii Engelm. e Eucalyptus grandis, utilizando-se 8% da formulação adesiva. As propriedades das chapas foram determinadas segundo a norma ASTM D-1037, de 1993. Observou-se que as propriedades das chapas foram superiores ao mínimo estabelecido pela norma ANSI/A 280.1-93, exceto no caso da resistência à umidade. Verificou-se, ainda, que o emprego de uma formulação adesiva contendo resina à base de uréia-formaldeído e tanino-formaldeído pode melhorar algumas propriedades.Bark tannins of Eucalyptus grandis and Eucalyptus pellita were extracted with 4,5% sodium sulfite in hot water solution for a period of three hours. Solution temperatures were 70 and 100ºC, for Eucalyptus grandis and Eucalyptus pellita bark respectively. Tannins were reacted with acetic acid and sodium sulfite to reduce adhesive viscosity. Adhesive formulations were prepared adding 0, 25, 50, 75 or 100% of tannin adhesives to the commercial urea-formaldehyde adhesive. Flakeboards were fabricated with 8% resin content. Board properties were determined according to ASTM D-1037 standards. Except for humidity, all board properties were superior to the values established by ANSI.A 208.1-93 commercial standard. Addition of tannins to the urea-formaldehyde adhesive improved some properties.

  5. Foliar Essential Oil Glands of Eucalyptus Subgenus Eucalyptus (Myrtaceae Are a Rich Source of Flavonoids and Related Non-Volatile Constituents.

    Directory of Open Access Journals (Sweden)

    Jason Q D Goodger

    Full Text Available The sub-dermal secretory cavities (glands embedded within the leaves of Eucalyptus (Myrtaceae were once thought to be the exclusive repositories of monoterpene and sesquiterpene oils. Recent research has debunked this theory and shown that abundant non-volatile compounds also occur within foliar glands. In particular, glands of four species in subgenus Eucalyptus contain the biologically active flavanone pinocembrin. Pinocembrin shows great promise as a pharmaceutical and is predominantly plant-sourced, so Eucalyptus could be a potential commercial source of such compounds. To explore this we quantified and assessed the purity of pinocembrin in glands of 11 species of E. subg. Eucalyptus using Electro-Spray Ionisation Liquid Chromatography Mass Spectrometry of acetonitrile extracts and Gas Chromatography Mass Spectrometry analyses of hexane extracts of isolated glands which were free from other leaf tissues. Our results showed that the glands of subgenus Eucalyptus contain numerous flavanones that are structurally related to pinocembrin and often present in much greater abundance. The maximum concentration of pinocembrin was 2 mg g-1 dry leaf found in E. stellulata, whereas that of dimethylpinocembrin (5,7-dimethoxyflavanone was 10 mg g-1 in E. oreades and that of pinostrobin (5-hydroxy-7-methoxyflavanone was 12 mg g-1 in E. nitida. We also found that the flavanones are exclusively located within the foliar glands rather than distributed throughout leaf tissues. The flavanones differ from the non-methylated pinocembrin in the degree and positions of methylation. This finding is particularly important given the attractiveness of methylated flavonoids as pharmaceuticals and therapeutics. Another important finding was that glands of some members of the subgenus also contain flavanone O-glucosides and flavanone-β-triketone conjugates. In addition, glands contain free β-triketones, β-triketone heterodimers and chromone C-glucosides. Therefore, the

  6. Foliar Essential Oil Glands of Eucalyptus Subgenus Eucalyptus (Myrtaceae) Are a Rich Source of Flavonoids and Related Non-Volatile Constituents.

    Science.gov (United States)

    Goodger, Jason Q D; Seneratne, Samiddhi L; Nicolle, Dean; Woodrow, Ian E

    2016-01-01

    The sub-dermal secretory cavities (glands) embedded within the leaves of Eucalyptus (Myrtaceae) were once thought to be the exclusive repositories of monoterpene and sesquiterpene oils. Recent research has debunked this theory and shown that abundant non-volatile compounds also occur within foliar glands. In particular, glands of four species in subgenus Eucalyptus contain the biologically active flavanone pinocembrin. Pinocembrin shows great promise as a pharmaceutical and is predominantly plant-sourced, so Eucalyptus could be a potential commercial source of such compounds. To explore this we quantified and assessed the purity of pinocembrin in glands of 11 species of E. subg. Eucalyptus using Electro-Spray Ionisation Liquid Chromatography Mass Spectrometry of acetonitrile extracts and Gas Chromatography Mass Spectrometry analyses of hexane extracts of isolated glands which were free from other leaf tissues. Our results showed that the glands of subgenus Eucalyptus contain numerous flavanones that are structurally related to pinocembrin and often present in much greater abundance. The maximum concentration of pinocembrin was 2 mg g-1 dry leaf found in E. stellulata, whereas that of dimethylpinocembrin (5,7-dimethoxyflavanone) was 10 mg g-1 in E. oreades and that of pinostrobin (5-hydroxy-7-methoxyflavanone) was 12 mg g-1 in E. nitida. We also found that the flavanones are exclusively located within the foliar glands rather than distributed throughout leaf tissues. The flavanones differ from the non-methylated pinocembrin in the degree and positions of methylation. This finding is particularly important given the attractiveness of methylated flavonoids as pharmaceuticals and therapeutics. Another important finding was that glands of some members of the subgenus also contain flavanone O-glucosides and flavanone-β-triketone conjugates. In addition, glands contain free β-triketones, β-triketone heterodimers and chromone C-glucosides. Therefore, the foliar glands

  7. Biomass in monospecific and mixed stands of eucalyptus and black wattle and corn in an agroforestry system

    Directory of Open Access Journals (Sweden)

    Márcio Viera

    2011-06-01

    Full Text Available This study aimed at quantifying the production and distribution of aboveground biomass from the plants in monospecific and mixed stands of eucalyptus (hybrid E. urophylla x E. grandis and black wattle (Acacia mearnsii and, of corn (Zea mays in agrosilvicultural systems. The biomass evaluation (leaf, branch, bark and wood from the forest species at 6 and 18 months of age were performed at the treatments: 100E (100% of eucalyptus + corn; - 100A (100% of black wattle + corn; - 50E:50A (50% of eucalyptus + 50% of black wattle + corn. The corn biomass evaluation (stem, leaves, straw, cob and grains was performed at treatments 100E; 100A; 50E:50A; 75E:25A (75% of eucalyptus + 25% of black wattle + corn; and - 25E:75A (25% of eucalyptus + 75% of black wattle + corn. The biomass production from eucalyptus and from the black wattle, in both monospecific and mixed planting, did not differ in any of the assessed ages but, when evaluated by plants compartments, it was verified an interspecific competitive interaction from the eucalyptus on the black wattle, reducing the formation of crown biomass. The total production of corn biomass in agrosilvicutural systems with eucalyptus and with black wattle in monospecific or mixed plantings did not differ in the studied treatments.

  8. Potential implications for expansion of freeze-tolerant eucalyptus plantations on water resources in the southern United States

    Science.gov (United States)

    James M. Vose; Chelcy F. Miniat; Ge Sun; Peter V. Caldwell

    2014-01-01

    The potential expansion of freeze-tolerant (FT) Eucalyptus plantations in the United States has raised concerns about the implications for water resources. Modeling was used to examine the potential effects of expanding the distribution of FT Eucalyptus plantations in US Department of Agriculture Plant Hardiness Zones 8b and...

  9. Fiscal 2000 report on result of the full-length cDNA structure analysis; 2000 nendo kanzen cho cDNA kozo kaiseki seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    This paper explains the results of research on full-length cDNA structure analysis for the period from April, 2000 to March, 2001. The outline of human genome sequence was published in June, 2000. In Japan, human gene analysis was such that, as the basic technology of the bio industry, a millennium project was decided in the budget of fiscal 2000. The full-length cDNA structure analysis is the core of the project. The libraries of cDNA were prepared using full-length and more than 4-5kbp-long cDNAs by oligo-capping method. It began from determining partial sequence data at end cDNA, and then, with new clones selected therefrom, full-length human cDNA sequence data were determined. The partial sequence data determined by fiscal 2000 were 1,035,000 clones while the full-length sequence data were 12,144 clones. The sequence data obtained were analyzed by homology search and translated into amino acid coding sequences, with predictions conducted on protein functions. A clustering method was examined that selects new clones from partial sequences. Database was constructed on gene expression profiles and disease-related gene sequence data. (NEDO)

  10. RICD: A rice indica cDNA database resource for rice functional genomics

    Directory of Open Access Journals (Sweden)

    Zhang Qifa

    2008-11-01

    Full Text Available Abstract Background The Oryza sativa L. indica subspecies is the most widely cultivated rice. During the last few years, we have collected over 20,000 putative full-length cDNAs and over 40,000 ESTs isolated from various cDNA libraries of two indica varieties Guangluai 4 and Minghui 63. A database of the rice indica cDNAs was therefore built to provide a comprehensive web data source for searching and retrieving the indica cDNA clones. Results Rice Indica cDNA Database (RICD is an online MySQL-PHP driven database with a user-friendly web interface. It allows investigators to query the cDNA clones by keyword, genome position, nucleotide or protein sequence, and putative function. It also provides a series of information, including sequences, protein domain annotations, similarity search results, SNPs and InDels information, and hyperlinks to gene annotation in both The Rice Annotation Project Database (RAP-DB and The TIGR Rice Genome Annotation Resource, expression atlas in RiceGE and variation report in Gramene of each cDNA. Conclusion The online rice indica cDNA database provides cDNA resource with comprehensive information to researchers for functional analysis of indica subspecies and for comparative genomics. The RICD database is available through our website http://www.ncgr.ac.cn/ricd.

  11. Consortium of eucalyptus with forage sorghum in semiarid of Minas Gerais State

    Directory of Open Access Journals (Sweden)

    Carlos Juliano Brant Albuquerque

    2017-11-01

    Full Text Available ABSTRACT: The objective was to estimate the wood yield and essential oil content in three clones of eucalyptus that were planted in four contrasting arrangements and intercropped with sorghum. Eucalyptus clones MA2001 (Eucalyptus camaldulensis x E. tereticornis, A144 (Eucalyptus urophylla x E. grandis, and GG100 (Eucalyptus urophylla x E. grandis, were planted in single rows (10x2m, double rows (2x3+15m and 2x3+20m; and, triple rows (2x3x2+10m in a randomized, complete block design experiment with four replicates. Our results demonstrated that planting spacing did not influence the essential oil yield or diameter at breast height in the clones. However, higher density plantings were shown to result in higher fresh weight of branches and leaves per plant. MA2001 grew taller, produced higher quantity of fresh biomass of branches and leaves per plant and volume of wood per hectare, and yielded more essential oil yield than the other clones. We concluded that MA2001 is the most suitable of the clones tested here for cultivation in water deficit conditions.

  12. Growth and nutrition of eucalyptus clones seedlings inoculated with mycorrhizal fungi

    Directory of Open Access Journals (Sweden)

    Francisco de Sousa Lima

    2014-06-01

    Full Text Available Eucalyptus is one of the most planted forest species, in Brazil, due to its rapid growth and high economic yield. Arbuscular mycorrhizal fungi improve the seedlings nutritional and phytosanitary status, besides increasing their resistance to biotic and abiotic stress. This study aimed to evaluate the effect of inoculation with arbuscular mycorrhizal fungi species on the growth and nutrition of different eucalyptus clones seedlings. The experiment was conducted under greenhouse conditions, in a randomized blocks design and a 5x5 factorial scheme (five fungal species and five eucalyptus clones, with five replications. In general, the mycorrhizal symbiosis significantly increased the growth and nutrition of eucalyptus seedlings, when compared to the non-inoculated seedlings. The most efficient interaction occured between the 2361 clone and the Entrophospora infrequens fungus, with increases of 107.3% and 120.6%, for the shoot and root dry biomass yield, and 107.7%, 94.1% and 103.3%, respectively for the accumulation of N, P and K in the seedlings shoots. All the fungal species studied showed a high absolute compatibility index with eucalyptus clones. The Glomus manihots and E. infrequens fungi presented a higher functional compatibility index with the clones tested. The 5204 clone showed 75% of compatibility with the fungi evaluated.

  13. Isotopic abundance of 13 C and contribution of eucalyptus biomass to soil organic matter conversion

    Directory of Open Access Journals (Sweden)

    Fabiane Figueiredo Severo

    Full Text Available ABSTRACT: It has become possible to evaluate the conversion of soil organic matter (SOM in pastures and arboreal crops due to the difference between the photosynthetic cycles of Eucalyptus (C3 and most grasses (C4. The auto analyzer method coupled to the IRMS (Isotope Ratio Mass Spectrometer in the present study evaluated the 13C content in soil profiles of Eucalyptus plantations of different ages (2, 10 and 21 years, in natural regeneration areas and natural grazing fields, and estimated the SOM conversion of each crop type of. The initial management of all sampled areas was natural pasture. The following profile layers were evaluated: 0-5, 5-10, 10-20, 20-30, 30-40, 40-50, 50-70 and 70-90cm, and the contribution of Eucalyptus biomass over the years of farming was estimated in the SOM conversion process. After 2 years of planting Eucalyptus, the beginning of pasture carbon conversion process occurred in the surface layer (0-5cm. Ten years after planting, the process of converting organic matter by arboreal crops reached the layers up to 20cm. After 21 years of planting and in natural regeneration areas, the entire profile has already been changed by planting Eucalyptus and native tree species.

  14. Encapsulated eucalyptus oil in ionically cross-linked alginate microcapsules and its controlled release.

    Science.gov (United States)

    Noppakundilograt, Supaporn; Piboon, Phianghathai; Graisuwan, Wilaiporn; Nuisin, Roongkan; Kiatkamjornwong, Suda

    2015-10-20

    Sodium alginate microcapsules containing eucalyptus oil were prepared by oil-in-water emulsification via Shirasu porous glass (SPG) membrane and cross-linked by calcium chloride (CaCl2). SPG membrane pore size of 5.2μm was used to control the size of eucalyptus oil microdroplets. Effects of sodium alginate, having a mannuronic acid/guluronic acid (M/G) ratio of 1.13, eucalyptus oil and CaCl2 amounts on microdroplet sizes and size distribution were elucidated. Increasing sodium alginate amounts from 0.1 to 0.5% (wv(-1)) sodium alginate, the average droplets size increased from 42.2±2.0 to 48.5±0.6μm, with CVs of 16.5±2.2 and 30.2±4.5%, respectively. CaCl2 successfully gave narrower size distribution of cross-linked eucalyptus oil microcapsules. The optimum conditions for preparing the microcapsules, oil loading efficiency, and controlled release of the encapsulated eucalyptus oil from the microcapsules as a function of time at 40°C were investigated. Release model for the oil from microcapsules fitted Ritger-Peppas model with non-Fickian transport mechanism. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. Path analysis of the energy density of wood in eucalyptus clones.

    Science.gov (United States)

    Couto, A M; Teodoro, P E; Trugilho, P F

    2017-03-16

    Path analysis has been used for establishing selection criteria in genetic breeding programs for several crops. However, it has not been used in eucalyptus breeding programs yet. In the present study, we aimed to identify the wood technology traits that could be used as the criteria for direct and indirect selection of eucalyptus genotypes with high energy density of wood. Twenty-four eucalyptus clones were evaluated in a completely randomized design with five replications. The following traits were assessed: basic wood density, total extractives, lignin content, ash content, nitrogen content, carbon content, hydrogen content, sulfur content, oxygen content, higher calorific power, holocellulose, and energy density. After verifying the variability of all evaluated traits among the clones, a two-dimensional correlation network was used to determine the phenotypic patterns among them. The obtained coefficient of determination (0.94) presented a higher magnitude in relation to the effect of the residual variable, and it served as an excellent model for explaining the genetic effects related to the variations observed in the energy density of wood in all eucalyptus clones. However, for future studies, we recommend evaluating other traits, especially the morphological traits, because of the greater ease in their measurement. Selecting clones with high basic density is the most promising strategy for eucalyptus breeding programs that aim to increase the energy density of wood because of its high heritability and magnitude of the cause-and-effect relationship with this trait.

  16. NUTRIENTS POOL IN CONSORTIA OF Eucalyptus urograndis, Acacia mearnsii AND Zea mays

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    Márcio Viera

    2013-08-01

    Full Text Available http://dx.doi.org/10.5902/1980509810543This study aimed to determine the nutrient pool in monospecific and mixed stands of Eucalyptus urograndis and Acacia mearnsii in a consortium with Zea mays.The amount determination of nutrients of forest species was carried out in the treatments: 100E (100% of eucalyptus; 100A (100% of black wattle and 50E:50A (50% of eucalyptus + 50% of black-wattle. On the other hand, for corn, it was carried out in all treatments (100E; 100A, 50E:50A; 75E:25A – 75% of eucalyptus + 25% black-wattle and 25E:75A – 25% of eucalyptus + 75% of black wattle. The delimitation adopted was the one of a randomized block with three replications. The magnitude of the nutrient pool in the agrossilvicultural systems biomass was: N> K > Ca > Mg > P > S, for macronutrients, and Mn > Fe > Zn > B > Cu, for micronutrients. Due to the great export of nutrients through the corn harvest, residues should be kept and it is necessary to make a nutritional reposition, mainly with P, N, K, S and Zn in the following crops, because of the higher amount that are exported with the extraction of the corn tang, which reaches 75.3; 60.6; 59.9; 55.8 e 53.8%, respectively, in relation to the total stocked in the biomass.

  17. Construction and characterization of cDNA library for IRM-2 mice

    International Nuclear Information System (INIS)

    Wang Qin; Li Jin; Song Li; Liu Qiang; Yue Jingyin; Mu Chuanjie; Tang Weisheng; Fan Feiyue

    2010-01-01

    Objective: To screen and isolate the radioresistance related genes of IRM-2 mice. Methods: cDNA library of IRM-2 mice was constructed by SMART technique. Total RNA was isolated from spleens of IRM-2 male mice. The first-strand cDNA was synthesized by using PowerScript reverse transcriptase, and double-strand cDNA was synthesized and amplified by long PCR. The PCR products were purified, digested with restriction enzyme Sfi I. The ds-cDNA fragment less than 500 bp was fractionated and ligated to the Sfi I-digested pDNR-LIB vector. The ligation mixture was transformed into E. coil DH5 α by electroporation transformation to generate the unamplified cDNA library. The quality of cDNA library was identified by PCR technique. 130 clones from cDNA library were sequenced and compared with GenBank database. Results: The cDNA library contained 2.25 x 10 6 independent clones with an average insert size of 1.2 kb. The ratio of recombination and full-length was 95% and 55%, respectively. 21 pieces of EST sequences from cDNA library were not the same as the known mice genes and registered into GenBank EST database, with registered number DW474856-DW474876. Conclusions: cDNA library of IRM-2 mice has been constructed successfully. 21 pieces of EST implies that radioresistance correlative genes may be in IRM-2 mice, which will lay a foundation for isolating and identifying radioresistance related genes in further study. (authors)

  18. Isolation of cDNA clones coding for human tissue factor: primary structure of the protein and cDNA

    International Nuclear Information System (INIS)

    Spicer, E.K.; Horton, R.; Bloem, L.

    1987-01-01

    Tissue factor is a membrane-bound procoagulant protein that activates the extrinsic pathway of blood coagulation in the presence of factor VII and calcium. λ Phage containing the tissue factor gene were isolated from a human placental cDNA library. The amino acid sequence deduced from the nucleotide sequence of the cDNAs indicates that tissue factor is synthesized as a higher molecular weight precursor with a leader sequence of 32 amino acids, while the mature protein is a single polypeptide chain composed of 263 residues. The derived primary structure of tissue factor has been confirmed by comparison to protein and peptide sequence data. The sequence of the mature protein suggests that there are three distinct domains: extracellular, residues 1-219; hydrophobic, residues 220-242; and cytoplasmic, residues 243-263. Three potential N-linked carbohydrate attachment sites occur in the extracellular domain. The amino acid sequence of tissue factor shows no significant homology with the vitamin K-dependent serine proteases, coagulation cofactors, or any other protein in the National Biomedical Research Foundation sequence data bank (Washington, DC)

  19. Optimization of agrobacterium tumefaciens mediated transformation in eucalyptus camaldulensis

    International Nuclear Information System (INIS)

    Ahad, A.; Maqbool, A.; Malik, K.A.

    2014-01-01

    This study was conducted to optimize Agrobacterium tumefaciens mediated transformation for Eucalyptus camaldulensis. Transformation was done by using LBA4404 containing binary plasmid pGA482 with uidA (Gus) gene under CamV35S promoter and nptII gene under nos promoter. For optimization, different explants (Cotyledonary leaves, plantlet leaves and hypocotyls of young In vitro plants and calli) with and without preculture were infected with a range of optical densities (O.D.600nm=0.3-0.6). Effect of different concentrations of Acetosyringone, infection time and co-cultivation time on transformation efficiency was evaluated. Confirmation of transformation was done through GUS histochemical staining and through PCR. Callogenesis and regeneration was found fast on MS medium containing 0.5 mg/L NAA and 1.5 mg/L BAP. Highest transformation efficiency was obtained with bacterial suspension of O.D.600nm = 0.5 for non-precultured explants and O.D.600nm=0.3 for precultured explants. (author)

  20. Rhizobacterial characterization for quality control of eucalyptus biogrowth promoter products

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    Talyta Galafassi Zarpelon

    Full Text Available Abstract Plant growth-promoting rhizobacteria strains from special formulations have been used to optimize eucalyptus cutting production. To undertake quality control for the formulated products, the rhizobacterial strains should be characterized to assess their purity and authentication. In the present study, we characterized nine strains of rhizobacteria, including three Bacillus subtilis (S1, S2 and 3918, two Pseudomonas sp. (MF4 and FL2, P. putida (MF2, P. fulva (Ca, Frateuria aurantia (R1, and Stenotrophomonas maltophilia (CIIb. The strains were differentiated by colony morphology after 24 h of incubation in three different solid state culture media (glucose-nutritive agar, 523 medium and yeast extract-mannitol agar, sensitivity to a panel of 28 antibiotics (expressed according to the formation of inhibition halos of bacterial growth in the presence of antibiotics, and PCR-RFLP profiles of the 16S rDNA gene produced using nine restriction enzymes. It was possible to differentiate all nine strains of rhizobacteria using their morphological characteristics and sensitivity to antibiotics. The molecular analysis allowed us to separate the strains CIIb, FL2 and R1 from the strains belonging to the genera Bacillus and Pseudomonas. By using these three methods concomitantly, we were able to determine strain purity and perform the authentication.

  1. Evaluation of bleachability on pine and eucalyptus kraft pulps

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    Marcela Freitas Andrade

    2013-09-01

    Full Text Available In recent decades, the pulp industry has been changing and improving its manufacturing processes in order to enhance production capacity, product quality and environmental performance. The aim of this study was to evaluate the bleachability effect on the efficient washing and alkaline leaching in eucalyptus and pine Kraft pulps using three different bleaching sequences: AD(EPD, A/D(EPDP and DHT(EPDP. This study was carried out in two stages. In the first part, the optimum conditions for pulp bleaching in order to achieve a brightness of 90% ISO were established. The second step was a comparative study between the pulps that received alkaline leaching and efficient washing with reference pulp (without treatment. The brightness, viscosity, kappa number and HexA in pulp were analyzed. The three sequences studied reached the desired brightness, but the sequence AD(EPD produced a lower reagent consumption for the same brightness. In the three sequences studied, the efficient washing of the pulp after oxygen delignification has contributed significantly to the removal of dissolved organic and inorganic materials in the pulp and the alkaline leaching decreased significantly the pulp kappa number due to a higher pulp delignification and bleachability.

  2. ANTHRAQUINONE ADDITION IN THE ALKALINE PULPING OF Eucalyptus saligna

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    Lúcia Helena Jerônimo

    2009-10-01

    Full Text Available The present work evaluated different alkaline pulping conditions for Eucalyptus saligna wood. The objective was to evaluate the influence of the anthraquinone (AQ on the reduction of the total reduced sulfur (TRS in the aerial emissions, by reducing the sulfidity. The experiment consisted of 8 cooking with active alkali varying from  19 to 21%, sulfidity from  0 to 20% and anthraquinone from 0 to 0.1%, aiming to obtain  kappa numbers in the range 15.5±1.5. The H factor was used to control the relation time/temperature along cooking. The addition of anthraquinone increased the delignification rate allowing sulfidity reduction. The kraft/AQ pulping with sulfidities of 5 and 10% presented satisfactory results, making it possible the replacement of conventional kraft cooking, giving a reduction in process sulfidity without harms in pulping. In spite of a slightly lower performance, the soda/AQ cooking has potential to be used where the smell is a critical problem in the industrial operation.

  3. Activated carbon from flash pyrolysis of eucalyptus residue

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    Grima-Olmedo C

    2016-09-01

    Full Text Available Forestry waste (eucalyptus sp was converted into activated carbon by initial flash pyrolysis followed carbonization and CO2 activation. These residues were obtained from a pilot plant in Spain that produces biofuel, the biochar represented 10–15% in weight. It was observed that the highest activation was achieved at a temperature of 800 °C, the specific surface increased with time but, on the contrary, high loss of matter was observed. At 600 °C, although there was an important increase of the specific surface and the volume of micropores, at this temperature it was observed that the activation time was not an influential parameter. Finally, at 400 °C it was observed that the activation process was not very significant. Assessing the average pore diameter it was found that the lowest value corresponded to the activation temperature of 600 °C, which indicated the development of microporosity. When the activation temperature increases up to 800 °C the pore diameter increased developing mesoporosity.

  4. Influence of temperature on products yield of Eucalyptus microcorys carbonization

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    Renato da Silva Vieira

    2013-03-01

    Full Text Available During charcoal production different products are formed. These products are influenced primarily by the temperature of carbonization. Given that charcoal is the main input in the production of pig iron in Brazil, this study evaluated the influence of final temperature of carbonization of the products generated and also the influence of the radial and longitudinal sampling on the yield of each product. Samples were taken from internal and external position along the radius and also from three different heights from four Eucalyptus microcorys trees. The samples were carbonized in an electric furnace with an experimental water-cooled condenser and a collecting bottle of condensable volatile materials. The final temperatures of carbonization were 500, 600, 700, 800 and 900°C. The gravimetric yield, tar and non-condensable gases were calculated. The results showed no difference in the gravimetric yield in the longitudinal and radial positions studied, while the tar yield and non-condensable gases showed temperature variations of 700°C and 800°C and the variation of the gravimetric yield temperatures between 500°C to 900°C was 15%, the change of yield of tar from the radial direction of sampling was on average 8%, the variation of the yield of non-condensable gases in a radial sampling was on average 16%.

  5. Eucalyptus Cloud to Remotely Provision e-Governance Applications

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    Sreerama Prabhu Chivukula

    2011-01-01

    Full Text Available Remote rural areas are constrained by lack of reliable power supply, essential for setting up advanced IT infrastructure as servers or storage; therefore, cloud computing comprising an Infrastructure-as-a-Service (IaaS is well suited to provide such IT infrastructure in remote rural areas. Additional cloud layers of Platform-as-a-Service (PaaS and Software-as-a-Service (SaaS can be added above IaaS. Cluster-based IaaS cloud can be set up by using open-source middleware Eucalyptus in data centres of NIC. Data centres of the central and state governments can be integrated with State Wide Area Networks and NICNET together to form the e-governance grid of India. Web service repositories at centre, state, and district level can be built over the national e-governance grid of India. Using Globus Toolkit, we can achieve stateful web services with speed and security. Adding the cloud layer over the e-governance grid will make a grid-cloud environment possible through Globus Nimbus. Service delivery can be in terms of web services delivery through heterogeneous client devices. Data mining using Weka4WS and DataMiningGrid can produce meaningful knowledge discovery from data. In this paper, a plan of action is provided for the implementation of the above proposed architecture.

  6. Colorimetry as grouping tool of eucalyptus clones wood

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    Márcio da Fonseca Martins

    2015-12-01

    Full Text Available The homogeneity of wood color in a batch to be marketed is of fundamental importance, as it will reflect in products quality resulting from its processing. In this context, this study aimed to evaluate, through colorimetric technique, the colorimetric parameters of Eucalyptus spp. wood from 25 clones and classify them into groups, according to color similarity degree. It was determined the lightness (L*, red-green color coordinate (a*, yellow-blue chromatic coordinate (b*, chromaticity (C* and ink angle (h. Radial and tangential faces and three positions in the radial direction were characterized using a colorimeter. Comparing to tangential planes, the results showed that radial plane presented larger values of L* and h in wood near the bark. Furthermore, it was observed higher values of L* in samples from intermediate radial positions. Finally, it was found that, as radial growth ocurred, developed timber showed less intense yellow shades. The definition of the wood color tones will be useful in timber market in the homogenization of their products, which will facilitate their marketing.

  7. Estimating chlorophyll content from Eucalyptus dunnii leaves by reflectance values

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    João Alexandre Lopes Dranski

    2016-06-01

    Full Text Available This study aimed to estimate photosynthetic pigments contents from leaves of Eucalyptus dunni Maiden based on values of reflectance spectra of red, green and blue colors obtained with a digital color analyzer. We collected fifty leaves from the lower third of the crown of twenty trees including young as well as mature leaves. From each leaf an area of 14 cm2 of the leaf blade was cut in which we measured reflectance values on the red, green and blue spectra with a portable digital colorimeter, obtained relative index of chlorophyll with a SPAD – 502 and determined the content of the chlorophyll a, b, and a + b by classic method of solvent extraction. We submitted the data to multiple linear regression and nonlinear analysis at 5% of error probability. It was evaluated the occurrence of multicollinearity. The negative exponential model resulted in good fit when data from red spectrum was used for chlorophyll a, green spectrum for chlorophyll b and a + b, making possible correlation coefficients between the estimated values and the extracted above 0.85. Except for the chlorophyll a content, the accuracy in estimates of photosynthetic pigments were higher than estimated by the chlorophyll meter, even with linearity between methods. Therefore, it is possible to estimate photosynthetic pigments on E. dunni leaves through values of red and green wavelengths from a digital color analyser.

  8. Containers of Attalea funifera fibers to produce eucalyptus seedlings

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    Andrea Vita Reis Mendonça

    2016-09-01

    Full Text Available The aim of this study was to assess the technical feasibility of using biodegradable containers made of fiber waste of Attalea funifera Martius to produce seedling of Eucalyptus camaldulensis Dehnh. The work was carried out in three stages: manufacture of piassava fiber containers, seedling production and field simulation. The experiment of seedling production was in completely randomized design, with two treatments (polyethylene tube and biodegradable container and 10 repetitions, with 64 seedlings per repetition. After 93 days, seedlings were evaluated based on quality variables. The simuation of initial growth of seedlings in the field consisted in planting seedlings in containers of 11L, in completely randomized design, with three treatments: seedlings produced in polyethylene tubes; seedlings produced in biodegradable containers, without removal of the container during planting; and seedlings produced in biodegradable containers, with removal of the container at planting, with ten repetitions, with one seedling by repetition. The biodegradable container withstood the production cycle and resulted in seedlings within acceptable standards quality. The use of biodegradable container, made of palm fibers, waived the removal of this vessel in the final planting.

  9. Essential oil of Algerian Eucalyptus citriodora: Chemical composition, antifungal activity.

    Science.gov (United States)

    Tolba, H; Moghrani, H; Benelmouffok, A; Kellou, D; Maachi, R

    2015-12-01

    Essential oil of Eucalyptus citriodora is a natural product which has been attributed for various medicinal uses. In the present investigation, E. citriodora essential oil was used to evaluate its antifungal effect against medically important dermatophytes. Essential oil from the Algerian E. citriodora leaves was analyzed by GC and GC/MS. The antifungal effect of E. citriodora essential oil was evaluated against four dermatophytes: Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum using disc diffusion method, disc volatilization method, and agar dilution method. The chemical composition of the oil revealed the presence of 22 compounds accounting for 95.27% of the oil. The dominant compounds were citronellal (69.77%), citronellol (10.63%) and isopulegol (4.66%). The disc diffusion method, MIC and MFC determination, indicated that E. citriodora essential oil had a higher antifungal potential against the tested strains with inhibition zone diameter which varied from (12 to 90mm) and MIC and MFC values ranged from (0.6 to 5μL/mL and 1.25 to 5μL/mL) respectively. The M. gypseum was the most resistant to the oil. The results of the present study indicated that E. citriodora essential oil may be used as a new antifungal agent recommended by the pharmaceutical industries. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  10. MOLYBDENUM CATALYZED ACID PEROXIDE BLEACHING OF EUCALYPTUS KRAFT PULP

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    Marcos S. Rabelo

    2008-08-01

    Full Text Available Molybdenum catalyzed peroxide bleaching (PMo Stage consists of pulp treatment with hydrogen peroxide under acidic conditions in the presence of a molybdenum catalyst. Molybdenum is applied in catalytic doses (50-200 mg/kg pulp and may originate from various sources, including (NH46Mo7O24.4H2O, Na2MoO4.2H2O, siliconmolybdate, etc. This work is aimed at optimizing the PMo stage and evaluating its industrial application in the OAZDP sequence. Optimum PMo stage conditions for bleaching eucalyptus pulp were 90 ºC, pH 3.5, 2 h, 0.1 kg/adt Mo and 5 kg/adt H2O2. The PMo stage was more efficient to remove pulp hexenuronic acids than lignin. Its efficiency decreased with increasing pH in the range of 1.5-5.5, while it increased with increasing temperature and peroxide and molybdenum doses. The application of the PMo stage as replacement for the A-stage of the AZDP sequence significantly decreased chlorine dioxide demand. The PMo stage caused a decrease of 20-30% in the generation of organically bound chlorine. The quality parameters of the pulp produced during the PMo stage mill trial were comparable to those obtained with the reference A-stage.

  11. Phenolic Compounds from the Leaves of Eucalyptus microcorys F. Muell.

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    Gilmara A. C. Fortes

    2015-04-01

    Full Text Available A new acylated glycoside, 4-O-( 4’,6’-di-O-galloyl- b - D -glucopyranosyl-trans-p-coumaric acid, named microcoryn ( 1 , together with sixteen known phenolic compounds, 5-O-(6'-O-galloyl- b - D -glucopyranosyl-gentisic acid (2, ellagic acid (3, gallic acid (4, kaempferol (5, quercetin (6, 3-O-galloyl- b - D -glucose (7, 2,3,6-tri-O-galloyl- b - D -glucose (8, 1,2,4,6-tetra-O-galloyl- b - D -glucose (9, 1,2,3,4,6-penta-O-galloyl- b - D -glucose (10, 4,6-hexahydroxydiphenoyl- b - D -glucose (11, gemin D (12, tellimagrandin I (13, tellimagrandin II (14, isocoriariin F (15, oenothein C (16, and oenothein B (17 were isolated from the leaves of Eucalyptus microcorys . The structure of the new compound was elucidated by spectroscopic data, especially by 2D NMR techniques. This is the first phytochemical investigation of this plant’s leaf extract.

  12. Phytotoxic effects of aqueous leaf extracts of two eucalyptus SPP. against parthenium hysterophorus L

    International Nuclear Information System (INIS)

    Javaid, A.; Shah, M.B.M.

    2007-01-01

    The present study was carried out to investigate the phytotoxic effect of aqueous leaf extracts of two eucalyptus species viz. E citriodora Hook and E. camaldulensis Dehnh. Against the germination and seeding growth of alien aggressive weed parthenium hysterophorus L. The experiment was conducted in department of Mycology and plant Pathology in 2006. Aqueous leaf extracts of 2, 4, 6, 8 and 10% (w/v) of the two aforementioned Eucalyptus species were employed in the present study. Extracts of 2-8% concentration of both the Eucalyptus species significantly suppressed germination of the target weed species. A 10% extract of both the species completely arrested the germination. Aqueous extracts also reduced the root and shoot length of parthenium. Effect of extracts on seedling biomass was insignificant. (author)

  13. Consumo Foliar de Eucalyptus spp. por Acromyrmex disciger (Mayr, 1887 (Hymenoptera: Formicidae

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    Wagner Calixto Morais

    2011-07-01

    Abstract. The productive potential of forest stands is reduced by pest occurrence among other factors. In Brazil, leaf-cutting ants are the most severe eucalypt pests. Acromyrmex disciger (Mayr is prevalent in the south east Brazil. However, scarce information about its potential damage for Eucalyptus forests is available. This work deals to quantifying the eucalypt leaf-consumption by such specie of leaf-cutting ant. Fresh leaves were taken from trees of Eucalyptus grandis W. Hill ex Maiden, Eucalyptus urophylla ST Blake, and hybrid E. urophylla x E. grandis and served to different colonies of A. disciger, during 24 hours period, over eight different times. Leaf-consumption was calculated throughout fresh weights of leaves, before and after ants foraging. Each colony of A. disciger consumed 38.8 ± 3.2 g e 22.0 ± 2.3 g of eucalypt leaves, per day.

  14. Expansion of eucalyptus culture in the municipalities of Minas Gerais and territorial management

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    João Batista Rezende

    2013-03-01

    Full Text Available This article deals with the development of monoculture index (MI of eucalyptus as a key element for the management of the territory, from the perspective of sustainable development. We analyzed the "geography" of the eucalyptus plantation in Minas Gerais, to support the planning, organization, control and use of territory. The Monoculture index proposed, which is an important tool for land management, was developed and validate by its application to municipalities of Minas Gerais state. It was shown that the culture of Eucalyptus represents a low rate of monoculture in the state of Minas Gerais and that the geography of this culture is compatible with sustainable territorial expansion. Therefore, these results contribute to the definition of public land management in Minas Gerais and the methodology used can be applied to other states.

  15. Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: a review.

    Science.gov (United States)

    Dhakad, Ashok K; Pandey, Vijay V; Beg, Sobia; Rawat, Janhvi M; Singh, Avtar

    2018-02-01

    The genus Eucalyptus L'Heritier comprises about 900 species, of which more than 300 species contain volatile essential oil in their leaves. About 20 species, within these, have a high content of 1,8-cineole (more than 70%), commercially used for the production of essential oils in the pharmaceutical and cosmetic industries. However, Eucalyptus is extensively planted for pulp, plywood and solid wood production, but its leaf aromatic oil has astounding widespread biological activities, including antimicrobial, antiseptic, antioxidant, chemotherapeutic, respiratory and gastrointestinal disorder treatment, wound healing, and insecticidal/insect repellent, herbicidal, acaricidal, nematicidal, and perfumes, soap making and grease remover. In the present review, we have made an attempt to congregate the biological ingredients of leaf essential oil, leaf oil as a natural medicine, and pharmacological and toxicological values of the leaf oil of different Eucalyptus species worldwide. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  16. Potential of Eucalyptus Oil as Repellent against House Rat, Rattus rattus

    Science.gov (United States)

    Thind, Ramandeep Kaur; Mahal, Amrit Kaur

    2014-01-01

    Rodent repellents are chemicals which by taste or odour or possibly by both will prevent animal from feeding or gnawing. Such substances may be used in protecting an area from rodent infestation or in protecting packaged food, packing materials, electric cables, and other important vulnerable materials. Mature and healthy house rat, Rattus rattus of both sexes, was exposed to 5, 10, and 20% eucalyptus oil applied as spray in laboratory pens in bichoice tests. Each concentration was applied through three different modes of application, that is, daily, once, and alternatively in a week. Repellent effect of the oil was assessed based on food consumption from treated and untreated sides for four days. In overall, food consumption was significantly (P eucalyptus oil. Present studies reveal the potential of eucalyptus oil in repelling away R. rattus; however, further studies may be conducted to enhance the persistence of repellent effect for longer period of time. PMID:24523633

  17. Effect of Echinophora platyloba , Stachys lavandulifolia, and Eucalyptus camaldulensis plants on Trichomonas vaginalis growth in vitro

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    Hossein Ali Youse

    2012-01-01

    Full Text Available Background: Trichomonas vaginalis is a protozoan parasite which causes vaginitis in women worldwide. Metronidazole with vast side effects is drug of choice for this infection. In search for an alternative drug, in this study the effect of three plants on Trichomonas vaginalis has been investigated in vitro. Materials and Methods: Alcoholic and watery extracts of Echinophora platyloba, Stachys lavandulifolia, and Eucalyptus camaldulensis were prepared. In TYIS culture medium containing alive Trichomonas vaginalis different concentrations of extracts of three plants were added. Following, 24, 48, and 72 h incubation the number of parasite in each test tube was counted. Results: Eucalyptus camaldulensis showed a strong effect on Trichomonas vaginalis growth. However, no significant effect was observed with Echinophora platyloba or Stachys lavandulifolia extracts. Conclusion: Eucalyptus camaldulensis can be considered as an alternative drug for treatment of infective vaginitis which is caused by bacteria, fungi and parasites.

  18. Asociacion de leveduras del genero Cryptococcus con especies de Eucalyptus en Santafe de Bogota Isolation of Cryptococcus sp. associated with Eucalyptus trees in Santafé de Bogota

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    A. Duarte

    1994-04-01

    Full Text Available El aislamiento de Cryptococcus neoformans var. gattii, serotipo B, a partir del medio ambiente se estableció inicialmente en Australia en 1989, en asocio con el Eucalyptus camaldulensis y posteriormente con E. tereticornis. Con estos hallazgos se postuló que desde allí, el hongo se ha podido exportar, por medio de las semillas contaminadas, a otras regiones geográficas, incluyendo Colombia. El objetivo de éste estudio fue identificar las levaduras del género Cryptococcus asociadas con especies de Eucalyptus sp., como primera evaluación en la ecología de C. neoformans var. gattii en nuestro país. Se realizó en Santafé de Bogotá, con una población de 100 árboles ubicados al centro, nororiente, oriente y occidente de la ciudad, recolectando de cada uno de ellos flores, frutos, hojas, cortezas y detritos; el procesamiento de las muestras incluyó extracción del material con una solución salina con antibióticos, siembra en medios selectivos e identificación de las especies con base en las características morfológicas, macro y microscópicas y bioquímicas. Se aislaron 27 cepas de Cryptococcus pertenecientes a 9 especies de Cryptococcus, a partir de 21 árboles ubicados en 5 zonas diferentes de la ciudad. Se aisló C. neoformans y se identificó como C. neoformans var. neoformans serotipo A. Estos datos iniciales son importantes como primera evaluación de la asociación de Cryptococcus sp. con los Eucalyptus en nuestro país.Environmental isolation of Cryptococcus neoformans var. gattii was first made in Australia in 1989 by ELLIS. He established a specific association with the tree species Eucalyptus camaldulensis and E. tereticornis. Based on his findings, ELLIS proposed that the fungus could be exported from Australia to others regions, including Colombia, by means of infected seeds. The purpose of this study was to isolate and identify Cryptococcus sp., associated with Eucalyptus trees; this is the first ecological evaluation

  19. BVOC emissions from 2 Asian Eucalyptus species,E.camadulensis and E.robusta

    Science.gov (United States)

    Tsui, J.; Guenther, A. B.; Chan, C. K.; Lau, A. P.

    2009-12-01

    Eucalyptus species dominate native forests in Australia and are planted over vast regions in Asia and other continents for afforestation and for pulp due to their fast growth rates. However, they have also been identified as high emitters of biogenic volatile organic compounds (BVOCs). BVOCs, when emitted to the atmosphere, react to form air pollutants such as ozone (O3) and secondary organic aerosols (SOA). The large areas of Eucalyptus forests in Australia and Asia, and high BVOC emission rates of Eucalyptus species, imply a potential significant effect of BVOC emissions from Eucalyptus on the air quality of these regions. A better understanding of BVOC emissions from this genus is thus needed. Here we present data of BVOC measurements from E.camadulensis and E.robusta. BVOC emissions of the 2 Eucalyptus species were measured by a branch enclosure approach in an environmental chamber, in which light and temperature were carefully controlled to mimic their changes throughout the day under natural conditions. E. camadulensis was found to emit isoprene, α-pinene, camphene and limonene, while E. robusta was found to emit isoprene, α-pinene, β-pinene, α-phellandrene, 3-carene and ocimene. Diurnal variations in BVOC emissions from the 2 species were observed. The 2 Eucalyptus species were also treated with methyl jasmonate (MeJA), a plant hormone which has found to induce elevated BVOC emissions similar to response to insect attacks in other plant species. The emission profiles of the 2 species before and after MeJA treatment were contrasted to examine the effects of the MeJA on their BVOC emissions. Acknowledgment: This work was supported by the General Research Fund of the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. 610909).

  20. Environmental effects on growth phenology of co-occurring Eucalyptus species.

    Science.gov (United States)

    Rawal, Deepa S; Kasel, Sabine; Keatley, Marie R; Aponte, Cristina; Nitschke, Craig R

    2014-05-01

    Growth is one of the most important phenological cycles in a plant's life. Higher growth rates increase the competitive ability, survival and recruitment and can provide a measure of a plant's adaptive capacity to climate variability and change. This study identified the growth relationship of six Eucalyptus species to variations in temperature, soil moisture availability, photoperiod length and air humidity over 12 months. The six species represent two naturally co-occurring groups of three species each representing warm-dry and the cool-moist sclerophyll forests, respectively. Warm-dry eucalypts were found to be more tolerant of higher temperatures and lower air humidity than the cool-moist eucalypts. Within groups, species-specific responses were detected with Eucalyptus microcarpa having the widest phenological niche of the warm-dry species, exhibiting greater resistance to high temperature and lower air humidity. Temperature dependent photoperiodic responses were exhibited by all the species except Eucalyptus tricarpa and Eucalyptus sieberi, which were able to maintain growth as photoperiod shortened but temperature requirements were fulfilled. Eucalyptus obliqua exhibited a flexible growth rate and tolerance to moisture limitation which enables it to maintain its growth rate as water availability changes. The wider temperature niche exhibited by E. sieberi compared with E. obliqua and Eucalyptus radiata may improve its competitive ability over these species where winters are warm and moisture does not limit growth. With climate change expected to result in warmer and drier conditions in south-east Australia, the findings of this study suggest all cool-moist species will likely suffer negative effects on growth while the warm-dry species may still maintain current growth rates. Our findings highlight that climate driven shifts in growth phenology will likely occur as climate changes and this may facilitate changes in tree communities by altering inter

  1. Eucalyptus Essential Oil as a Natural Food Preservative: In Vivo and In Vitro Antiyeast Potential

    Science.gov (United States)

    Bukvicki, Danka; Gottardi, Davide; Malik, Anushree; Guerzoni, Maria Elisabetta

    2014-01-01

    In this study, the application of eucalyptus essential oil/vapour as beverages preservative is reported. The chemical composition of eucalyptus oil was determined by gas chromatography-mass spectrometry (GC-MS) and solid phase microextraction GC-MS (SPME/GC-MS) analyses. GC-MS revealed that the major constituents were 1,8-cineole (80.5%), limonene (6.5%), α-pinene (5%), and γ-terpinene (2.9%) while SPME/GC-MS showed a relative reduction of 1,8-cineole (63.9%) and an increase of limonene (13.8%), α-pinene (8.87%), and γ-terpinene (3.98%). Antimicrobial potential of essential oil was initially determined in vitro against 8 different food spoilage yeasts by disc diffusion, disc volatilization, and microdilution method. The activity of eucalyptus vapours was significantly higher than the eucalyptus oil. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) varied from 0.56 to 4.50 mg/mL and from 1.13 to 9 mg/mL, respectively. Subsequently, the combined efficacy of essential oil and thermal treatment were used to evaluate the preservation of a mixed fruit juice in a time-dependent manner. These results suggest eucalyptus oil as a potent inhibitor of food spoilage yeasts not only in vitro but also in a real food system. Currently, this is the first report that uses eucalyptus essential oil for fruit juice preservation against food spoiling yeast. PMID:25177704

  2. Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves.

    Science.gov (United States)

    Sebei, Khaled; Sakouhi, Fawzi; Herchi, Wahid; Khouja, Mohamed Larbi; Boukhchina, Sadok

    2015-01-19

    In this paper, we have studied the essential oils chemical composition of the leaves of seven Eucalyptus species developed in Tunisia. Eucalyptus leaves were picked from trees growing in different arboretums in Tunisia. Choucha and Mrifeg arboretums located in Sedjnene, region of Bizerte (Choucha: E. maideni, E. astrengens et E. cinerea; Mrifeg : E. leucoxylon), Korbous arboretums located in the region of Nabeul, North East Tunisia with sub-humid bioclimate, (E. lehmani), Souiniet-Ain Drahem arboretum located in region of Jendouba (E. sideroxylon, E. bicostata). Essential oils were individually tested against a large panel of microorganisms including Staphylococcus aureus (ATCC 6539), Escherichia coli (ATCC 25922), Enterococcus faecalis (ATCC29212), Listeria ivanovii (RBL 30), Bacillus cereus (ATCC11778). The yield of essential oils ranged from 1.2% to 3% (w/w) for the different Eucalyptus species. All essential oils contain α-pinene, 1,8-cineol and pinocarveol-trans for all Eucalyptus species studied. The 1,8-cineol was the major compound in all species (49.07 to 83.59%). Diameter of inhibition zone of essential oils of Eucalyptus species varied from 10 to 29 mm. The largest zone of inhibition was obtained for Bacillus cereus (E. astrengens) and the lowest for Staphylococcus aureus (E. cinerea). The essential oils from E. maideni, E. astrengens, E. cinerea (arboretum of Bizerte), E. bicostata (arboretum of Aindraham) showed the highest antibacterial activity against Listeria ivanovii and Bacillus cereus. The major constituents of Eucalyptus leaves essential oils are 1,8-cineol (49.07 to 83.59%) and α-pinene (1.27 to 26.35%). The essential oils from E. maideni, E. astrengens, E. cinerea, E. bicostata showed the highest antibacterial activity against Listeria ivanovii and Bacillus cereus, they may have potential applications in food and pharmaceutical products.

  3. Eucalyptus Essential Oil as a Natural Food Preservative: In Vivo and In Vitro Antiyeast Potential

    Directory of Open Access Journals (Sweden)

    Amit Kumar Tyagi

    2014-01-01

    Full Text Available In this study, the application of eucalyptus essential oil/vapour as beverages preservative is reported. The chemical composition of eucalyptus oil was determined by gas chromatography-mass spectrometry (GC-MS and solid phase microextraction GC-MS (SPME/GC-MS analyses. GC-MS revealed that the major constituents were 1,8-cineole (80.5%, limonene (6.5%, α-pinene (5%, and γ-terpinene (2.9% while SPME/GC-MS showed a relative reduction of 1,8-cineole (63.9% and an increase of limonene (13.8%, α-pinene (8.87%, and γ-terpinene (3.98%. Antimicrobial potential of essential oil was initially determined in vitro against 8 different food spoilage yeasts by disc diffusion, disc volatilization, and microdilution method. The activity of eucalyptus vapours was significantly higher than the eucalyptus oil. Minimum inhibitory concentration (MIC and minimum fungicidal concentration (MFC varied from 0.56 to 4.50 mg/mL and from 1.13 to 9 mg/mL, respectively. Subsequently, the combined efficacy of essential oil and thermal treatment were used to evaluate the preservation of a mixed fruit juice in a time-dependent manner. These results suggest eucalyptus oil as a potent inhibitor of food spoilage yeasts not only in vitro but also in a real food system. Currently, this is the first report that uses eucalyptus essential oil for fruit juice preservation against food spoiling yeast.

  4. Sequence of a cloned cDNA encoding human ribosomal protein S11

    Energy Technology Data Exchange (ETDEWEB)

    Lott, J B; Mackie, G A

    1988-02-11

    The authors have isolated a cloned cDNA that encodes human ribosomal protein (rp) S11 by screening a human fibroblast cDNA library with a labelled 204 bp DNA fragment encompassing residues 212-416 of pRS11, a rat rp Sll cDNA clone. The human rp S11 cloned cDNA consists of 15 residues of the 5' leader, the entire coding sequence and all 51 residues of the 3' untranslated region. The predicted amino acid sequence of 158 residues is identical to rat rpS11. The nucleotide sequence in the coding region differs, however, from that in rat in the first position in two codons and in the third position in 44 codons.

  5. Mouse tetranectin: cDNA sequence, tissue-specific expression, and chromosomal mapping

    DEFF Research Database (Denmark)

    Ibaraki, K; Kozak, C A; Wewer, U M

    1995-01-01

    regulation, mouse tetranectin cDNA was cloned from a 16-day-old mouse embryo library. Sequence analysis revealed a 992-bp cDNA with an open reading frame of 606 bp, which is identical in length to the human tetranectin cDNA. The deduced amino acid sequence showed high homology to the human cDNA with 76......(s) of tetranectin. The sequence analysis revealed a difference in both sequence and size of the noncoding regions between mouse and human cDNAs. Northern analysis of the various tissues from mouse, rat, and cow showed the major transcript(s) to be approximately 1 kb, which is similar in size to that observed...

  6. Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA

    DEFF Research Database (Denmark)

    Stangegaard, Michael; Dufva, I.H.; Dufva, Hans Martin

    2006-01-01

    oligonucleotides (pentadecamers) consistently, yielded at least 2 fold as much cDNA as did random hexamers using either-poly(A) RNA or an amplified version of messenger RNA (aRNA) as a template. The cDNA generated using pentadecamers did not differ in size distribution or the amount of incorporated label compared...... with cDNA generated with random hexamers. The increased efficiency of priming using random pentadecamers resulted in reverse transcription of > 80% of the template aRNA, while random hexamers induced reverse transcription of only 40% of the template aRNA. This suggests a better coverage...... that random pentadecamers can replace random hexamers in reverse transcription reactions on both poly(A) RNA and amplified RNA, resulting in higher cDNA yields and quality....

  7. Development of Gene Expression Fingerprints for Identification of Environmental Contaminants Using cDNA Arrays

    National Research Council Canada - National Science Library

    Inouye, L

    2004-01-01

    ...) to develop cDNA array-based assays that map gene expression from contaminant exposures. Results substantiate that distinct gene expression profiles exist for major contaminant classes such as PARs, PCBs, and PCDD/Fs...

  8. 3G vector-primer plasmid for constructing full-length-enriched cDNA libraries.

    Science.gov (United States)

    Zheng, Dong; Zhou, Yanna; Zhang, Zidong; Li, Zaiyu; Liu, Xuedong

    2008-09-01

    We designed a 3G vector-primer plasmid for the generation of full-length-enriched complementary DNA (cDNA) libraries. By employing the terminal transferase activity of reverse transcriptase and the modified strand replacement method, this plasmid (assembled with a polydT end and a deoxyguanosine [dG] end) combines priming full-length cDNA strand synthesis and directional cDNA cloning. As a result, the number of steps involved in cDNA library preparation is decreased while simplifying downstream gene manipulation, sequencing, and subcloning. The 3G vector-primer plasmid method yields fully represented plasmid primed libraries that are equivalent to those made by the SMART (switching mechanism at 5' end of RNA transcript) approach.

  9. Compaction and soil fertility after eucalyptus harvesting using Feller Buncher and Skidder

    Directory of Open Access Journals (Sweden)

    Ana Lúcia Piedade Sodero Martins Pincelli

    2014-06-01

    Full Text Available This study analyzed, the impact of Feller Buncher and Skidder traffic in harvesting areas of eucalyptus in Mogi Guaçu, considering the compaction and fertilization effects in the range of soil next to the carrier during the cycle of forest growth. An increase in soil compaction, caused by machinery traffic in topsoil (0-10 cm, was observed in the area recently harvested. The soils of the study areas, with eucalyptus 1.4 and 6.0 years old, showed good fertility conditions, especially the older area, where decomposition of forest residues possibly contributed to such fertility.

  10. Qualidade de juntas coladas com lâminas de madeira oriundas de três regiões do tronco de Eucalyptus grandis, Eucalyptus saligna e Pinus elliottii Quality of wood joints glued with wood veneers from three trunk regions of Eucalyptus grandis, Eucalyptus saligna and Pinus elliottii

    Directory of Open Access Journals (Sweden)

    Benedito Rocha Vital

    2006-08-01

    Full Text Available Este experimento teve como objetivo avaliar a resistência de juntas coladas formadas pelas combinações de lâminas provenientes de três posições no tronco da madeira de Eucalyptus grandis, Eucalyptus saligna e Pinus elliottii. Foram empregados adesivos à base de poliacetato de vinila de média e alta viscosidade e resorcinol-formaldeído nas gramaturas de 150 g/m², em face simples para o poliacetato de vinila de média e alta viscosidades e 300 g/m², em face dupla, para o adesivo resorcinólico. O teor médio de umidade das lâminas, no momento da colagem, foi igual a 14%. Os valores médios mais elevados de resistência ao cisalhamento foram obtidos nas juntas produzidas com madeira de Eucalyptus saligna, coladas com adesivos de poliacetato de média viscosidade e resorcinol-formaldeído. A maior porcentagem de falha profunda na madeira foi obtida em juntas de madeira de Pinus elliottii, unidas com adesivo de poliacetato de alta viscosidade, seguidas das juntas de Eucalyptus grandis e coladas com adesivo de poliacetato de média viscosidade. As combinações de lâminas oriundas das seguintes posições no tronco: medula e casca, intermediária e casca e casca e casca resultaram em linhas de cola com maiores resistências ao cisalhamento.The objective of this work was to evaluate the shear strength of glued wood joints from pith, outer and intermediary wood of Eucalyptus saligna, Eucalyptus grandis and Pinus elliottii. High and medium viscosity polyvinyl acetate and resorcinol-phenol adhesives were applied at spread rate of 150 g/m² in single line and at spread rate of 300 g/m² in double glue line for the resorcinolic adhesive. The mean wood moisture content was 14%. Higher shear strength was obtained with Eucalyptus saligna veneer glued with medium viscosity polyvinyl resorcinolic adhesive. The highest percentage of wood failure was found on Pinus elliottii veneer glued with high viscosity polyvinyl acetate adhesive followed by

  11. Microwave-Assisted Extraction of Essential Oil from Eucalyptus: Study of the Effects of Operating Conditions

    Directory of Open Access Journals (Sweden)

    A.A. Saoud

    2006-12-01

    Full Text Available Classical extraction of essential oil such as Soxhlet and steam distillation is still a formidable and time-solvent consuming. Microwave assisted process (MAP is used to accelerate the extraction process of target compounds. It can be used for the extraction of compounds from various plants and animal tissues, or the extraction of undesirable components from raw materials. The investigation of microwave extraction of eucalyptus (globules essential oil using ethanol as solvent was carried out. The influence of material (eucalyptus/solvent (ethanol ratio, required doses of microwave, and time of microwave exposure on extraction efficiency, was studied.

  12. Produção de biomassa e remoção de nutrientes em povoamentos de Eucalyptus camaldulensis Dehnh, Eucalyptus grandis hill ex Maiden e Eucalyptus torelliana F. Muell, plantados em Anhembí, SP.

    Directory of Open Access Journals (Sweden)

    Mauro Valdir Schumacher

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 Neste trabalho estudou-se a distribuição de biomassa e a quantidade de nutrientes estocados nos diferentes compartimentos (folhas, ramos, casca e lenho das árvores de Eucalyptus camaldulensis Dehnh, Eucalyptus grandis Hill ex Maiden e Eucalyptus torelliana F. Muell com 9, 9 e 12 anos de idade, respectivamente, plantados em solos de textura arenosa e baixa fertilidade, em Anhembi - SP. A espécie E. grandis foi a que apresentou a maior produção de biomassa para todos os compartimentos analisados, com exceção dos ramos grossos. Nas três espécies o tronco (casca + lenho representou em média 90% da biomassa acima do solo. Os nutrientes concentraram-se de forma decrescente nas folhas, ramos, casca e lenho. As copas das árvores foram responsáveis pelo acúmulo de, aproximadamente, 24% dos nutrientes contidos na biomassa total das árvores. Na casca encontram-se as maiores quantidades de cálcio, aproximadamente 60% do total. A espécie E. grandis removeu do solo a maior quantidade de nutrientes, desenvolvendo através da queda de folhedo menores quantidades que as outras espécies. Desta forma cuidados especiais deverão ser dispensados para garantir a produtividade das rotações futuras.

  13. Radioactive cDNA microarray (II): Gene expression profiling of antidepressant treatment by human cDNA microarray

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Ji Hye; Kang, Rhee Hun; Ham, Byung Joo; Lee, Min Su; Shin, Kyung Ho; Choe, Jae Gol; Kim, Meyoung Kon [College of Medicine, Univ. of Korea, Seoul (Korea, Republic of)

    2003-07-01

    Major depressive disorder is a prevalent psychiatric disorder in primary care, associated with impaired patient functioning and well-being. Fluoxetine is a selective serotonin-reuptake inhibitors (SSRIs) and is a commonly prescribed antidepressant compound. Its action is primarily attributed to selective inhibition of the reuptake of serotonin (5-hydroxytryptamine) in the central nervous system. Objectives ; the aims of this study were two-fold: (1) to determine the usefulness for investigation of the transcription profiles in depression patients, and (2) to assess the differences in gene expression profiles between positive response group and negative response groups by fluoxetine treatment. This study included 53 patients with major depression (26 in positive response group with antidepressant treatment, 27 in negative response group with antidepressant treatment), and 53 healthy controls. To examine the difference of gene expression profile in depression patients, radioactive complementary DNA microarrays were used to evaluate changes in the expression of 1,152 genes in total. Using 33p-labeled probes, this method provided highly sensitive gene expression profiles including brain receptors, drug metabolism, and cellular signaling. Gene transcription profiles were classified into several categories in accordance with the antidepressant gene-regulation. The gene profiles were significantly up-(22 genes) and down-(16 genes) regulated in the positive response group when compared to the control group. Also, in the negative response group, 35 genes were up-regulated and 8 genes were down-regulated when compared to the control group. Consequently, we demonstrated that radioactive human cDNA microarray is highly likely to be an efficient technology for evaluating the gene regulation of antidepressants, such as selective serotonin-reuptake inhibitors (SSRIs), by using high-throughput biotechnology.

  14. Radioactive cDNA microarray (II): Gene expression profiling of antidepressant treatment by human cDNA microarray

    International Nuclear Information System (INIS)

    Lee, Ji Hye; Kang, Rhee Hun; Ham, Byung Joo; Lee, Min Su; Shin, Kyung Ho; Choe, Jae Gol; Kim, Meyoung Kon

    2003-01-01

    Major depressive disorder is a prevalent psychiatric disorder in primary care, associated with impaired patient functioning and well-being. Fluoxetine is a selective serotonin-reuptake inhibitors (SSRIs) and is a commonly prescribed antidepressant compound. Its action is primarily attributed to selective inhibition of the reuptake of serotonin (5-hydroxytryptamine) in the central nervous system. Objectives ; the aims of this study were two-fold: (1) to determine the usefulness for investigation of the transcription profiles in depression patients, and (2) to assess the differences in gene expression profiles between positive response group and negative response groups by fluoxetine treatment. This study included 53 patients with major depression (26 in positive response group with antidepressant treatment, 27 in negative response group with antidepressant treatment), and 53 healthy controls. To examine the difference of gene expression profile in depression patients, radioactive complementary DNA microarrays were used to evaluate changes in the expression of 1,152 genes in total. Using 33p-labeled probes, this method provided highly sensitive gene expression profiles including brain receptors, drug metabolism, and cellular signaling. Gene transcription profiles were classified into several categories in accordance with the antidepressant gene-regulation. The gene profiles were significantly up-(22 genes) and down-(16 genes) regulated in the positive response group when compared to the control group. Also, in the negative response group, 35 genes were up-regulated and 8 genes were down-regulated when compared to the control group. Consequently, we demonstrated that radioactive human cDNA microarray is highly likely to be an efficient technology for evaluating the gene regulation of antidepressants, such as selective serotonin-reuptake inhibitors (SSRIs), by using high-throughput biotechnology

  15. Cloning of cDNA encoding steroid 11β-hydroxylase (P450c11)

    International Nuclear Information System (INIS)

    Chua, S.C.; Szabo, P.; Vitek, A.; Grzeschik, K.H.; John, M.; White, P.C.

    1987-01-01

    The authors have isolated bovine and human adrenal cDNA clones encoding the adrenal cytochrome P-450 specific for 11β-hydroxylation (P450c11). A bovine adrenal cDNA library constructed in the bacteriophage λ vector gt10 was probed with a previously isolated cDNA clone corresponding to part of the 3' untranslated region of the 4.2-kilobase (kb) mRNA encoding P450c11. Several clones with 3.2-kb cDNA inserts were isolated. Sequence analysis showed that they overlapped the original probe by 300 base pairs (bp). Combined cDNA and RNA sequence data demonstrated a continuous open reading frame of 1509 bases. P450c11 is predicted to contain 479 amino acid residues in the mature protein in addition to a 24-residue amino-terminal mitochondrial signal sequence. A bovine clone was used to isolate a homologous clone with a 3.5-kb insert from a human adrenal cDNA library. A region of 1100 bp was 81% homologous to 769 bp of the coding sequence of the bovine cDNA except for a 400-bp segment presumed to be an unprocessed intron. Hybridization of the human cDNA to DNA from a panel of human-rodent somatic cell hybrid lines and in situ hybridization to metaphase spreads of human chromosomes localized the gene to the middle of the long arm of chromosome 8. These data should be useful in developing reagents for heterozygote detection and prenatal diagnosis of 11β-hydroxylase deficiency, the second most frequent cause of congenital adrenal hyperplasia

  16. CDNA cloning, characterization and expression of an endosperm-specific barley peroxidase

    DEFF Research Database (Denmark)

    Rasmussen, Søren Kjærsgård; Welinder, K.G.; Hejgaard, J.

    1991-01-01

    A barley peroxidase (BP 1) of pI ca. 8.5 and M(r) 37000 has been purified from mature barley grains. Using antibodies towards peroxidase BP 1, a cDNA clone (pcR7) was isolated from cDNA expression library. The nucleotide sequence of pcR7 gave a derived amino acid sequence identical to the 158 C...

  17. cDNA fingerprinting of osteoprogenitor cells to isolate differentiation stage-specific genes.

    OpenAIRE

    Candeliere, G A; Rao, Y; Floh, A; Sandler, S D; Aubin, J E

    1999-01-01

    A cDNA fingerprinting strategy was developed to identify genes based on their differential expression pattern during osteoblast development. Preliminary biological and molecular staging of cDNA pools prepared by global amplification PCR allowed discrim-inating choices to be made in selection of expressed sequence tags (ESTs) to be isolated. Sequencing of selected ESTs confirmed that both known and novel genes can be isolated from any developmental stage of interest, e.g. from primitive progen...

  18. cDNA cloning and mRNA expression of heat shock protein 70 gene ...

    African Journals Online (AJOL)

    In this study, the full-length heat shock protein 70 of Tegillarca granosa was cloned from cDNA library by rapid amplification of cDNA end (RACE). The open reading frame (ORF) of heat shock protein 70 was 1968 bp, and it encoded a protein of 655 amino acids with a predicted molecular weight of 71.48 kDa and an ...

  19. [Primary culture of cat intestinal epithelial cell and construction of its cDNA library].

    Science.gov (United States)

    Ye, L; Gui-Hua, Z; Kun, Y; Hong-Fa, W; Ting, X; Gong-Zhen, L; Wei-Xia, Z; Yong, C

    2017-04-12

    Objective To establish the primary cat intestinal epithelial cells (IECs) culture methods and construct the cDNA library for the following yeast two-hybrid experiment, so as to screen the virulence interaction factors among the final host. Methods The primary cat IECs were cultured by the tissue cultivation and combined digestion with collagenase XI and dispase I separately. Then the cat IECs cultured was identified with the morphological observation and cyto-keratin detection, by using goat anti-cyto-keratin monoclonal antibodies. The mRNA of cat IECs was isolated and used as the template to synthesize the first strand cDNA by SMART™ technology, and then the double-strand cDNAs were acquired by LD-PCR, which were subsequently cloned into the plasmid PGADT7-Rec to construct yeast two-hybrid cDNA library in the yeast strain Y187 by homologous recombination. Matchmaker™ Insert Check PCR was used to detect the size distribution of cDNA fragments after the capacity calculation of the cDNA library. Results The comparison of the two cultivation methods indicated that the combined digestion of collagenase XI and dispase I was more effective than the tissue cultivation. The cat IECs system of continuous culture was established and the cat IECs with high purity were harvested for constructing the yeast two-hybrid cDNA library. The library contained 1.1×10 6 independent clones. The titer was 2.8×10 9 cfu/ml. The size of inserted fragments was among 0.5-2.0 kb. Conclusion The yeast two-hybrid cDNA library of cat IECs meets the requirements of further screen research, and this study lays the foundation of screening the Toxoplasma gondii virulence interaction factors among the cDNA libraries of its final hosts.

  20. LEDGF/p75 Deficiency Increases Deletions at the HIV-1 cDNA Ends.

    Science.gov (United States)

    Bueno, Murilo T D; Reyes, Daniel; Llano, Manuel

    2017-09-15

    Processing of unintegrated linear HIV-1 cDNA by the host DNA repair system results in its degradation and/or circularization. As a consequence, deficient viral cDNA integration generally leads to an increase in the levels of HIV-1 cDNA circles containing one or two long terminal repeats (LTRs). Intriguingly, impaired HIV-1 integration in LEDGF/p75-deficient cells does not result in a correspondent increase in viral cDNA circles. We postulate that increased degradation of unintegrated linear viral cDNA in cells lacking the lens epithelium-derived growth factor (LEDGF/p75) account for this inconsistency. To evaluate this hypothesis, we characterized the nucleotide sequence spanning 2-LTR junctions isolated from LEDGF/p75-deficient and control cells. LEDGF/p75 deficiency resulted in a significant increase in the frequency of 2-LTRs harboring large deletions. Of note, these deletions were dependent on the 3' processing activity of integrase and were not originated by aberrant reverse transcription. Our findings suggest a novel role of LEDGF/p75 in protecting the unintegrated 3' processed linear HIV-1 cDNA from exonucleolytic degradation.