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Sample records for ethylenediaminetetraacetic acid edta

  1. Evaluation of ethylenediaminetetraacetic acid (EDTA) solution and gel for smear layer removal.

    Science.gov (United States)

    Dotto, Sidney Ricardo; Travassos, Rosana Maria Coelho; de Oliveira, Elias Pandonor Motcy; Machado, Manoel Eduardo de Lima; Martins, José Luiz

    2007-08-01

    The purpose of this in vitro study was to compare the efficacy of 24% ethylenediaminetetraacetic acid (EDTA) gel and 17% EDTA solution in cleaning dentine walls after root canal instrumentation. Thirty human canine teeth were divided into three groups of 10 teeth each. In Group 1, 1% sodium hypochlorite was used as the irrigating solution; in Group 2, 1% sodium hypochlorite was used with 17% EDTA solution; and in Group 3, 1% sodium hypochlorite was used with 24% EDTA gel. The presence of a smear layer was analysed after instrumentation using scanning electron microscopy. The Kruskal-Wallis test revealed a statistical difference (P 0.05). The results indicate that 1% sodium hypochlorite alone does not remove the smear layer and that there was no statistical difference between EDTA gel and EDTA solution in smear layer removal.

  2. Direct analysis of ethylenediaminetetraacetic acid (EDTA) on concrete by reactive-desorption electrospray ionization mass spectrometry.

    Science.gov (United States)

    Lebeau, D; Reiller, P E; Lamouroux, C

    2015-01-01

    Analysis of organic ligands such as ethylenediaminetetraacetic acid (EDTA) is today an important challenge due to their ability to increase the mobility of radionuclides and metals. Reactive desorption electrospray ionization mass spectrometry (reactive-DESI-MS) was used for direct analysis of EDTA on concrete samples. EDTA forms complexes and those with Fe(III) ions are among the most thermodynamically favored. This complexing capacity was used to improve the specific detection of EDTA directly on a concrete matrix by doping the solvent spray of DESI with a solution of FeCl3 to selectively create the complex between EDTA and Fe(III). Thus, EDTA sensitivity was largely improved by two orders of magnitude with reactive-DESI-MS experiments thanks to the specific detection of EDTA as a [EDTA-4H+Fe(III)](-) complex. The proof of principle that reactive DESI can be applied to concrete samples to detect EDTA has been demonstrated. Its capacity for semi-quantitative determination and localization of EDTA under ambient conditions and with very little sample preparation, minimizing sample manipulations and solvent volumes, two important conditions for the development of new methodologies in the field of analytical chemistry, has been shown. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Sodium citrate blood contamination by K2 -ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing.

    Science.gov (United States)

    Lima-Oliveira, G; Salvagno, G L; Danese, E; Favaloro, E J; Guidi, G C; Lippi, G

    2015-06-01

    The potential cross-contamination of additives between primary blood tubes is a well-known problem during sample collection. The aim of this study was to assess the impact of citrated blood contamination with different amounts of dipotassium ethylenediaminetetraacetic (K2 EDTA blood) on activated partial thromboplastin time (APTT), prothrombin time (PT), and fibrinogen. Blood was collected from 15 ostensibly healthy volunteers into four 0.109 m citrate blood tubes followed by one K2 EDTA blood tube. The citrate tubes of each subject were pooled and divided in five aliquots. The whole blood of the K2 EDTA tube was then added in scalar amounts to autologous citrated blood aliquots, to obtain K2 EDTA contamination ranging from 0% to 43%, and thus mimic potential pre-analytical contamination. A statistically and clinically significant prolongation was observed for both APTT and PT between 29% and 43% K2 EDTA contamination, whereas the decrease of fibrinogen values became statistically and clinically significant at 43% K2 EDTA contamination. The results of this investigation show that contamination of citrated blood with as much as 29% of K2 EDTA blood generates a significant bias in results of routine clotting assays. This has serious implications for patient safety and management. © 2014 John Wiley & Sons Ltd.

  4. Isolation of living Algae growing in the shells of Molluscs and Barnacles with EDTA (ethylenediaminetetraacetic acid)

    NARCIS (Netherlands)

    Prud’homme van Reine, W.F.; Hoek, van den C.

    1966-01-01

    Several decalcifying mixtures or aqueous solutions of inorganic or organic acids are generally used for releasing algae growing in the shells of molluscs and barnacles, for instance dilute hydrochloric, nitric, citric, or acetic acid (4), a mixture of nitric acid, chromic acid and alcolhol (1),

  5. 51Cr-ethylenediaminetetraacetic acid absorption test

    International Nuclear Information System (INIS)

    Aabakken, L.

    1989-01-01

    The 51 Cr-ethylenediaminetetraacetic acid (EDTA) absorption test was evaluated in 83 healthy, male volunteers. Base-line 24 h excretion after peroral administration ranged from 0.88% to 7.96%, with a higher median absorption than reported by most authors (2.45%). However, the reproducibility and stability of the method and the reproducibility of the results were satisfactory. Urinary excretion after intraduodenal installation (n=18) was comparable to that seen after peroral test dose administration, indicating a limited significance of gastric 51 Cr-EDTA absorption under normal conditions. In 16 subjects a single intake of alcohol immediately before the test gave a modest and short-lasting increase in 51 Cr-EDTA absorption. No correlation was seen to body mass index, creatinine clearance, urinary volume, or small-bowel transit time, possibly reducing the number of confounding factors in the evaluation of absorption data. A small but significant negative correlation was, however, found to body surface area and age

  6. Effects of ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA) derivatives on penetration of /sup 169/Yb and /sup 144/Ce into the rat offspring

    Energy Technology Data Exchange (ETDEWEB)

    Baltrukiewicz, Z; Burakowski, T; Derecki, J

    1976-01-01

    Penetration of radioactive ytterbium-169 and cerium-144 into fetuses was determined at the end of pregnancy and penetration into suckling rats was studied during feeding with the milk of exposed mothers when EDTA or DTPA derivatives were being administered. Injection of ytterbium-169 as a complex with EDTA or DTPA or injection of Na/sub 2/Ca EDTA or Na/sub 3/Ca DTPA 1h after administration of cerium-144 to mothers reduced penetration of both radionuclides into offsprings in relation to the animals receiving no complex compounds. It was observed that the action of DTPA was stronger than that of EDTA. Passage of ytterbium with milk and across the placenta was greater than the passage of cerium.

  7. Effects of ethylenediaminetetraacetic acid (EDTA)and diethylenetriaminepentaacetic acid (DTPA) derivatives on penetration of ytterbium-169 and cerium-144 into the rat offspring

    Energy Technology Data Exchange (ETDEWEB)

    Baltrukiewicz, Z; Burakowski, T; Derecki, J [Wojskowy Inst. Higieny i Epidemiologii, Warsaw (Poland)

    1976-01-01

    Penetration of radioactive ytterbum-169 and cerium-144 into fetuses was determined at the end of pregnancy and penetration into the organism of suckling rats was studied during feeding with the milk of exposed mothers when EDTA or DTPA derivatives were being administered. Injection of ytterbum-169 as a complex with EDTA or DTPA or injection of Na/sub 2/Ca EDTA or Na/sub 3/Ca DTPA 1h after administration of cerium-144 to mothers reduced penetration of both radionuclides into offsprings in relation to the animals receiving no complex compounds. It was observed that the action of DTPA was stronger than that of EDTA. Passage of ytterbium with milk and across the placenta was greater than the passage of cerium.

  8. Coulometric Titration of Ethylenediaminetetraacetate (EDTA) with Spectrophotometric Endpoint Detection: An Experiment for the Instrumental Analysis Laboratory

    Science.gov (United States)

    Williams, Kathryn R.; Young, Vaneica Y.; Killian, Benjamin J.

    2011-01-01

    Ethylenediaminetetraacetate (EDTA) is commonly used as an anticoagulant in blood-collection procedures. In this experiment for the instrumental analysis laboratory, students determine the quantity of EDTA in commercial collection tubes by coulometric titration with electrolytically generated Cu[superscript 2+]. The endpoint is detected…

  9. Simultaneous administration of lactulose and 51Cr-ethylenediaminetetraacetic acid

    International Nuclear Information System (INIS)

    Jenkins, A.P.; Nukajam, W.S.; Menzies, S.; Creamer, B.

    1992-01-01

    In normal adults intestinal permeation of ingested 51 Cr-ethylenediaminetetraacetic acid (EDTA) is greater than that of lactulose. This difference is abolished in patients with ileostomies, suggesting that it results from colonic permeation of 51 Cr-EDTA, which, unlike lactulose, resists bacterial degradation. To investigate the effect of an increase in colonic permeability on absorption of the two molecules, lactulose and 51 Cr-EDTA were given orally in isosmolar solution to 11 patients with colitis, and their 24-h urinary excretion measured. By comparison the effect of an increase in small-intestinal permeability induced by ingestion of a hyperosmolar solution was measured in 10 healthy adults. Hyperosmolar stress increased the 24-h urinary excretion of 51 Cr-EDTA above the normal mean + 2 standard deviations in all 10 healthy subjects, and in all of these excretion of lactulose was also increased. In contrast, although seven colitics had a urinary excretion of 51 Cr-EDTA above the normal mean + 2 SD, in only two of these patients was recovery of lactulose increased. This suggests that simultaneous administration of lactulose and 51 Cr-EDTA may enable permeability changes affecting the colon alone to be distinguished from those involving the small intestine. 15 refs., 1 fig., 5 tabs

  10. Effect of Ethylenediaminetetraacetic Acid and Ammonium Oxalate ...

    African Journals Online (AJOL)

    Effect of Ethylenediaminetetraacetic Acid and Ammonium Oxalate on the ... The test plant was sown in aluminium-polluted soil (conc. ... The perseverance of the test plant in the aluminium spiked soil is an indication of adaptation to the stress ...

  11. Effects of combination of ethylenediaminetetraacetic acid and ...

    African Journals Online (AJOL)

    This experiment was conducted to evaluate the combined effects of ethylene diamine tetraacetic acid (EDTA) and microbial phytase (MP) on the serum concentration and digestibility of some minerals in broiler chicks. This experiment was conducted using 360 Ross-308 male broiler chicks in a completely randomized ...

  12. Effect of sodium iron ethylenediaminetetraacetic acid on the absorption of various trace elements in anemic rats

    International Nuclear Information System (INIS)

    Igarashi, Kaori; Sasaki, Ayako; Yoda, Yoko; Inage, Hiroko; Nakanishi, Yukiko; Kimura, Shuichi; Yanagiya, Takahiro; Hirunuma, Rieko; Enomoto, Shuichi

    2001-01-01

    Iron deficiency in developing countries is attributed to the bioavailability of iron derived from staple food such as grains, vegetables and legumes. Sodium iron ethylenediaminetetraacetic acid (NaFeEDTA), a strong metal chelator, is one of the food additives for iron fortification and it has been for improvement of iron deficiency in the world. However, the effect of NaFeEDTA on the absorption of trace elements other than Fe has been poorly understood. In the present study, to investigate the effect of NaFeEDTA on the absorption of trace elements, we determined the uptake rate of various metals using a multitracer technique. The uptake rates of Zn, Co, and Na in rats fed with NaFeEDTA diet were significantly lower than those in rats fed with ferrous sulfate (FS) diet, suggesting that iron fortification by NaFeEDTA lowers the bioavailability of various elements compared with FS. On the other hand, iron fortification using the FS diet in the presence of tannic acid decreased the bioavailability of Zn and Rb. However, no effect of tannic acid on the uptake rate of metal was observed in NaFeEDTA diet, suggesting that iron fortification using NaFeEDTA is less affected by tannic acid than that using the FS diet. These results indicate that iron fortification using NaFeEDTA is an effective method for improving iron deficiency. (author)

  13. The effects of EDTA (Ethylenediaminetetraacetate on the growth of Thalassiosira weissflogii

    Directory of Open Access Journals (Sweden)

    Selin Sayın

    2015-12-01

    Full Text Available Demir (Fe biyolojik olaylarda görev yapan önemli bir eser metaldir ve pek çok organizma için hayatidir. Fe’in biyolojik olaylarda hala tam olarak bilinmeyen işlevleri, sudaki kullanılabilir formları ve alımını etkileyen faktörlerin belirlenmesi konularına katkıda bulunmak amacıyla yürütülen çalışmada, 10 nM demir (Fe içeren modifiye F/2 ortamında, suni ligand EDTA (Ethylenediaminetetraacetate (100µM nın, Thalassiosira weissflogii’nin büyümesine etkileri araştırılmıştır. Denemenin yürütüldüğü ortamda, 18 ± 2 °C sıcaklık ve 80µmol m-2 s-1 aydınlanma şiddeti sürekli olacak şekilde sağlanmıştır. Denemede kullanılan deniz suyu, makro besleyiciler ve EDTA, Chelex-100 reçinesinden geçirilmiş, kullanılan bütün malzemeler ise asit ile yıkanarak eser metallerinden arındırılmıştır. Denemenin sonunda, EDTA nın, T. weissflogii kültürlerinde, hücre yoğunluğu, klorofil-a miktarı, büyüme hızı ve büyümeye paralel olarak artan pH değerlerini etkilemediği (p>0.05 saptanırken hücresel Fe miktarları arasında farklılık olduğu (p

  14. Enhancement of phloem exudation from Fraxinus uhdei Wenz. (evergreen ash) using ethylenediaminetetraacetic acid

    International Nuclear Information System (INIS)

    Costello, L.R.; Bassham, J.A.; Calvin, M.

    1982-01-01

    Ethylenediaminetetraacetic acid (EDTA) enhanced the exudation of 14 C-labeled assimilates from excised leaflets and whole plant specimens of Fraxinus uhdei Wenz. A 2 millimolar EDTA concentration was found to be most effective in promoting exudation from excised leaflets, while 10 millimolar EDTA was most effective in whole plants experiments. Exudation rate reached a maximum after 24 hours in both experiments. The continuous presence of EDTA throughout the treatment period was required for maximum exudation from excised leaflets. Stachyose, raffinose, verbascose, and sucrose were the principal compounds found to occur in exudate samples. These compounds are typically transported in sieve elements of various Fraxinus species suggesting the exudate was of phloem orgin. Electron microscope studies of petiolule sieve plate pores from excisd leaflets showed substantially less callose appearing after treatment with EDTA than after H 2 O treatment. It is suggested that EDTA enhances phloem exudation by inhibiting or reducing callose formation in sieve plate pores. The exudation enhancement technique described for whole plant specimens is suggested as a useful means of collecting phloem sap and studying translocation in woody plants

  15. Ethylenediaminetetraacetic acid induces antioxidant and anti-inflammatory activities in experimental liver fibrosis.

    Science.gov (United States)

    González-Cuevas, J; Navarro-Partida, J; Marquez-Aguirre, A L; Bueno-Topete, M R; Beas-Zarate, C; Armendáriz-Borunda, J

    2011-01-01

    Experimental liver fibrosis induced by carbon tetrachloride (CCl(4)) is associated with oxidative stress, lipid peroxidation, and inflammation. This work was focused on elucidating the anti-inflammatory and antioxidant effects of ethylenediaminetetraacetic acid (EDTA) in this model of hepatotoxicity. Wistar male rats were treated with CCl(4) and EDTA (60, 120, or 240 mg/kg). Morphometric analyses were carried out in Masson's stained liver sections to determine fibrosis index. Coagulation tests prothrombin time (PT) and partial thromboplastin time (PTT) were also determined. Gene expression for transforming growth factor beta (TGF-beta1), alpha1(I) procollagen gene (alpha1 Col I), tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), and superoxide dismutase (SOD) was monitored by real-time PCR. Antioxidant effect of EDTA was measured by its effects on lipid peroxidation; biological activity of ceruloplasmin (Cp), SOD, and catalase (Cat) were analyzed by zymography assays. Animals with CCl(4)-hepatic injury that received EDTA showed a decrement in fibrosis (20%) and lipid peroxidation (22%). The mRNA expression for TNF-alpha (55%), TGF-beta1 (50%), IL-6 (52%), and alpha1 Col I (60%) was also decreased. This group of animals showed increased Cp (62%) and SOD (25%) biological activities. Coagulation blood tests, Cat activity, and gene expression for SOD were not modified by EDTA treatment. This study demonstrates that EDTA treatment induces the activity of antioxidant enzymes, decreases lipid peroxidation, hepatic inflammation, and fibrosis in experimental liver fibrosis induced by CCl(4).

  16. In vitro dissolution of calcium oxalate stones with ethylenediaminetetraacetic acid and snake venom thrombin-like enzyme.

    Science.gov (United States)

    Zhou, Xiang-Jun; Zhang, Jie; Zhang, Ci; Xu, Chang-Geng

    2014-01-01

    The aim of this study was to determine the feasibility of using snake venom thrombin-like enzyme (SVTLE) and/or ethylenediaminetetraacetic acid (EDTA) to dissolve calcium oxalate stones in vitro. Seven calcium oxalate stones were incubated with various chemolytic agents [EDTA, Tris-HCl/EDTA (TE) buffer or SVTLE diluted in TE buffer]. The pH, calcium concentration, stone weight and stone surface integrity were recorded, as well as related pathological changes to bladder mucosae. Compared to all other solutions, those containing SVTLE and buffered EDTA had higher concentrations of mobilized calcium and caused significantly more stone weight loss, stone fragility and gaps in the calcium crystals. Also, there were no adverse pathological effects on rabbit bladder mucosae from any of the solutions. The data indicate that buffered EDTA and SVTLE can be used to dissolve calcium oxalate stones and, at the concentrations used here, do not damage tissue. 2013 S. Karger AG, Basel.

  17. Effects of EDTA-Na (Na ethylenediaminetetraacetate) upon the metabolism of radiostrontium and radioyttrium in mice

    Energy Technology Data Exchange (ETDEWEB)

    Kikuchi, T; Wakisaka, G; Kono, T; Hiroaki, A; Yamamasu, T; Sugawa, I

    1954-01-01

    The toxicity of EDTA-Na, inert Sr (NO/sub 3/)/sub 2/ and Ba (NO/sub 3/)/sub 2/ has been examined. Simultaneous injection of EDTA-Na showed no significant effect upon the distribution of radio-Sr in the bones of mice. The distribution of radio-Y in the bones of mice tended to decrease following the simultaneous subcutaneous injection of /sup 91/Y and EDTA-Na.

  18. Effect of Ethylenediaminetetraacetic Acid and Ammonium Oxalate ...

    African Journals Online (AJOL)

    ADOWIE PERE

    acid and ammonium oxalate on the prevalence of microorganisms and removal of aluminum in soil by bitter leaf plant (Vernonia ... highest accumulation of aluminium in the root (16.92mg/kg); however concentrations of aluminium in the roots were .... whereas the sulphate was 13.75mg/kg. Table 2: The total colony count of ...

  19. Effects of combination of ethylenediaminetetraacetic acid and ...

    African Journals Online (AJOL)

    Administrator

    2010-12-27

    Dec 27, 2010 ... Key words: Ethylene diamine tetraacetic acid, microbial phytase, zinc, copper, manganese. .... At day 44, chrome oxide (Cr2O3) was added to all diets at 0.1% level as a .... The hydrolysis of phytate phosphorus by chicks and.

  20. Effect of the association between citric acid and EDTA on root surface etching.

    Science.gov (United States)

    Manzolli Leite, Fabio Renato; Nascimento, Gustavo Giacomelli; Manzolli Leite, Elza Regina; Leite, Amauri Antiquera; Cezar Sampaio, Josá Eduardo

    2013-09-01

    This study aims to compare the clot stabilization on root surfaces conditioned with citric acid and ethylenediamine-tetraacetic acid (EDTA). Scaled root samples (n = 100) were set in fve groups: group I-control group (saline solution); group II (24% EDTA); group III (25% citric acid); group IV (EDTA + citric acid); group V (citric acid + EDTA). Fifty samples were assessed using the root surface modifcation index (RSMI). The other 50 received a blood drop after conditioning. Clot formation was assessed using blood elements adhesion index (BEAI). A blind examiner evaluated photomicrographs. Statistical analysis considered p EDTA employment before citric acid (group-IV) reduced clot formation in comparison to citric acid use alone (group-III). Root conditioning with citric acid alone and before EDTA had the best results for smear layer removal and clot stabilization. EDTA inhibited clot stabilization on root surface and must have a residual activity once it has diminished clot adhesion to root even after citric acid conditioning. Thus, EDTA can be used to neutralize citric acid effects on periodontal cells without affecting clot stabilization. Clinical signifcance: To demonstrate that citric acid use on root surfaces previously affected by periodontal disease may favor clot stabilization and may have a benefcial effect on surgical outcomes. Also, EDTA can be used to neutralize citric acid effects on periodontal cells.

  1. Use of Pyrolyzed Iron Ethylenediaminetetraacetic Acid Modified Activated Carbon as Air–Cathode Catalyst in Microbial Fuel Cells

    KAUST Repository

    Xia, Xue

    2013-08-28

    Activated carbon (AC) is a cost-effective catalyst for the oxygen reduction reaction (ORR) in air-cathode microbial fuel cells (MFCs). To enhance the catalytic activity of AC cathodes, AC powders were pyrolyzed with iron ethylenediaminetetraacetic acid (FeEDTA) at a weight ratio of FeEDTA:AC = 0.2:1. MFCs with FeEDTA modified AC cathodes and a stainless steel mesh current collector produced a maximum power density of 1580 ± 80 mW/m2, which was 10% higher than that of plain AC cathodes (1440 ± 60 mW/m 2) and comparable to Pt cathodes (1550 ± 10 mW/m2). Further increases in the ratio of FeEDTA:AC resulted in a decrease in performance. The durability of AC-based cathodes was much better than Pt-catalyzed cathodes. After 4.5 months of operation, the maximum power density of Pt cathode MFCs was 50% lower than MFCs with the AC cathodes. Pyridinic nitrogen, quaternary nitrogen and iron species likely contributed to the increased activity of FeEDTA modified AC. These results show that pyrolyzing AC with FeEDTA is a cost-effective and durable way to increase the catalytic activity of AC. © 2013 American Chemical Society.

  2. Chemical and physical characteristics of a soybean beverage with improved flavor by addition of ethylenediaminetetraacetic acid

    Directory of Open Access Journals (Sweden)

    Guzmán, Carlos A.

    2000-10-01

    Full Text Available A new method to obtain a soybean (SB with improved flavor characteristics was developed by adding ethylenediaminetetraacetic acid (EDTA. The SB was evaluated for pH, viscosity and density, as well as for protein, oil and ash contents, fatty acid composition and lipoxygenase activity. A water/bean ratio of 4.5:1 was selected because it provided the best protein (4.22 g 100 ml-1 and total solids (8.80 g 100 ml-1 contents. Sensory ratings for flavor and aroma intensities were also determined and compared with those of a commercial soymilk and a soybean beverage without EDTA. Samples from SB had the lowest ratings for green/beany and rancid flavors. The results indicated that the addition of EDTA may reduce off-flavors in soybean products.Se ha desarrollado un nuevo método para obtener un alimento líquido de soja (ALS con mejores cualidades organolépticas mediante adición de ácido etilendiaminotetraacético (EDTA. En el producto obtenido se evaluaron el pH, la viscosidad y la densidad, así como también los contenidos de proteínas, grasas y cenizas, la composición en ácidos grasos y la actividad de lipoxigenasa. Se seleccionó una relación agua/haba de soja equivalente a 4.5:1 puesto que la misma produjo los más altos contenidos de proteínas (4.22 g 100 ml-1 y sólidos totales (8.80 g 100 ml-1. Se realizó una evaluación sensorial, mediante pruebas de aceptabilidad, del aroma y sabor del ALS y se compararon con los de una «leche de soja» comercial y un alimento líquido de soja sin agregado de EDTA. Las muestras de ALS presentaron las evaluaciones más bajas para sabores rancio y «afrijolado». Los resultados obtenidos indicaron que la adición de EDTA puede reducir sabores desagradables en productos de soja.

  3. Comparison between lactulose/mannitol and 51Cr-ethylenediaminetetraacetic acid/14C-mannitol methods for intestinal permeability

    International Nuclear Information System (INIS)

    Blomquist, L.; Bark, T.; Hedenborg, G.; Svenberg, T.; Norman, A.

    1993-01-01

    Urinary excretion of lactulose and mannitol, determined by gas-liquid chromatography, was compared with that of 51 Cr-ethylenediaminetetraacetic acid (EDTA) and 14 C-mannitol for measurement of intestinal permeability in 28 healthy humans. The 0- to 6-h excretion values for unlabelled and labelled mannitol (marker of transcellular permeability) were normally distributed, whereas excretion values for lactulose and 51 Cr-EDTA (markers of paracellular permeability) were shewly distributed, as were the lactulose to mannitol and 51 Cr-EDTA to 14 C-mannitol ratios. Excretion of the transcellular markers, but not of the paracullular markers was significantly correlated to urinary volume; correction for urinary volume resulted in decreased test variability. Significant correlation was found between lactulose and 51 Cr-EDTA excretion and between mannitol and 14 C-mannitol excretion, but not between the lactulose to mannitol and 51 Cr-EDTA to 14 C-mannitol ratios. Inter- and intraindividual test variability was greater for each chemically determined marker than for the corresponding isotope-labelled marker. Similarly, variability was greater for each paracellular marker than for the corresponding transcellular marker and for each paracellular/transcellular marker ratio, than for the transcellular marker alone. Variability of mannitol excretion was increased by the frequent presence of food-derived mannitol in the urine. 23 refs., 5 figs., 2 tabs

  4. The effect of ethylenediaminetetraacetic acid on heat resistance and recovery of Clostridium sporogenes PA 3679 spores treated in HTST conditions.

    Science.gov (United States)

    Silla Santos, M H; Torres Zarzo, J

    1997-03-03

    The effect of ethylenediaminetetraacetic acid (EDTA) on the heat resistance of Clostridium sporogenes PA 3679 spores was studied. EDTA was added to heating substrates and recovery media in order to establish which stage of the heat treatment registered the greatest EDTA activity. The heating substrates assayed were phosphate buffer (pH 7.0) and white asparagus purée, at natural pH (5.8) and acidified with citric acid and glucono-delta-lactone (GDL) to pH 5.5, 5.0 and 4.5. Recovery of survivors was carried out in MPA3679A medium in various conditions of acidification with citric and GDL (250 and 500 ppm), at pH 7.5 6.5 and 6.0. The results show greater activity of EDTA on spores when it was applied in recovery of heat injured spores, than during heating. The strongest influence of EDTA during heating was found in phosphate buffer (pH 7.0), with the effect being most evident at 121 and 126 degrees C, and in asparagus purée, at 121 degrees C and pH 5.8 rather than acidified. In recovery, the inhibiting activity of EDTA was more evident in spores subjected to more severe heat treatment, either by increasing the exposure time or by raising the temperature to 130 or 135 degrees C. The pH level of the recovery medium also affected the antimicrobial activity of EDTA, which had a greater inhibiting effect at pH 7.5 than at lower pH levels (6.5, 6.0).

  5. Disodium N,N-bis-(dithiocarboxy)ethanediamine: synthesis, performance, and mechanism of action toward trace ethylenediaminetetraacetic acid copper (II).

    Science.gov (United States)

    Xiao, Xiao; Ye, Maoyou; Yan, Pingfang; Qiu, Yiqin; Sun, Shuiyu; Ren, Jie; Dai, Yongkang; Han, Dajian

    2016-10-01

    A new effective multi-dithiocarbamate heavy metal precipitant, disodium N,N-bis-(dithiocarboxy) ethanediamine (BDE), was synthesized by mixing ethanediamine with carbon disulfide under alkaline conditions, and it was utilized for removing trace ethylenediaminetetraacetic acid copper (II) (EDTA-Cu) from wastewater. Its structure was confirmed by ultraviolet spectra, Fourier transform infrared spectra, scanning electron microscopy, thermogravimetric analysis, and elemental analysis. The removal performance of EDTA-Cu by BDE was evaluated according to BDE dosage, initial concentration, pH, and reaction time through single-factor experiments. With the optimized conditions of a pH range of 3-9, dosage ratio of BDE/Cu of 1:1, PAM dosage of 1 mg/L, and reaction time of 4 min, the removal efficiency of Cu(2+) was more than 98 % from simulated wastewater containing EDTA-Cu with initial concentrations of 5-100 mg/L. Treatment of actual EDTA-Cu wastewater showed that BDE performed superior effectiveness, and the average residential concentration of Cu(2+) was 0.115 mg/L. Besides, the stability of chelated precipitate and the reaction mechanism of BDE and EDTA-Cu were also introduced. The toxicity characteristic leaching procedure (TCLP) and semi-dynamic leaching test (SDLT) indicated that the chelated precipitate was non-hazardous and stable in weak acid and alkaline conditions. The BDE reacts with EDTA-Cu at a stoichiometric ratio, and the removal of Cu(2+) was predominantly achieved through the replacement reaction of BDE and EDTA-Cu.

  6. Effect of QMix, peracetic acid and ethylenediaminetetraacetic acid on calcium loss and microhardness of root dentine

    Science.gov (United States)

    Taneja, Sonali; Kumari, Manju; Anand, Surbhi

    2014-01-01

    Objectives: The objective of this in vitro study was to assess the effect of different chelating agents on the calcium loss and its subsequent effect on the microhardness of the root dentin. Materials and Methods: Ten single rooted lower premolars were selected. The teeth were decoronated and thick transverse sections of 2 mm were obtained from the coronal third of the root. Each section was then divided into four quarters, each part constituting a sample specimen from the same tooth for each group. The treatment groups were: Group 1 (Control): 5% Sodium hypochlorite (NaOCl) for 5 min + distilled water for 5 min; Group 2: 5% NaOCl for 5 min + 17% ethylenediaminetetraacetic acid (EDTA) for 5 min; Group 3: 5% NaOCl for 5 min + 2.25% Peracetic acid (PAA) for 5 min and Group 4: 5% NaOCl for 5 min + QMix for 5 min respectively. The calcium loss of the samples was evaluated using the Atomic Absorption Spectrophotometer followed by determination of their microhardness using Vickers Hardness Tester. Data was analyzed using one-way ANOVA, Post hoc Tukey test and Pearson correlation. Results: The maximum calcium loss and minimum microhardness was observed in Group 3 followed by Group 2, Group 4 and Group 1. There was a statistically significant difference between all the groups except between Groups 2 and 4. Conclusions: Irrigation with NaOCl + 2.25% PAA caused the maximum calcium loss from root dentin and reduced microhardness. A negative correlation existed between the calcium loss and reduction in the microhardness of root dentin. PMID:24778513

  7. Effect of the impregnation of carbon cloth with ethylenediaminetetraacetic acid on its adsorption capacity for the adsorption of several metal ions

    International Nuclear Information System (INIS)

    Afkhami, Abbas; Madrakian, Tayyebeh; Amini, Azadeh; Karimi, Ziba

    2008-01-01

    Effect of loading of C-cloth with ethylenediaminetetraacetic acid (EDTA) on the adsorption capacity for the adsorption of several metal cations was studied. The concentration of ions in the solution was monitored using atomic absorption spectrometry. The adsorption isotherm data for the cations were derived at 25 deg. C and treated according to Langmuir and Freundlich models and was found that for most of the investigated cations Langmuir model was more successful. Adsorption capacities determined from Langmuir isotherms. Loading of the adsorbent with EDTA increased the adsorption capacity for the adsorption of all of the investigation ions

  8. Comparison of the efficacy of ethylenediaminetetraacetic acid and tetracycline hydrochloride as root conditioning agents: An in vitro study

    Directory of Open Access Journals (Sweden)

    Tarun Nanda

    2012-01-01

    Full Text Available Objective: Root surfaces of periodontitis-affected teeth are hypermineralized and contaminated with cytotoxic and other biologically active substances. Various root conditioning agents have been recommended as an adjunct to mechanical root surface debridement to expose dentin collagen and cementum bound proteins. The aim of the present in vitro study was to compare the efficacy of ethylenediaminetetraacetic acid (EDTA and tetracycline hydrochloride (HCl as root conditioning agents on planed root surfaces. Materials and Methods: A total of 20 human maxillary anterior teeth indicated for extraction due to chronic periodontitis were collected and root planed. The teeth were sectioned and specimens were divided into two groups - Group I and II. Group I dentin specimens were treated with EDTA and Group II specimens were treated with tetracycline HCl solution at a concentration of 10% by active burnishing technique for 3 min. The root surface samples were then examined by scanning electron microscope. Results: The results of this study showed that EDTA and tetracycline HCl were equally effective in removing the smear layer. It was observed that the total and patent dentinal tubules were more in number in teeth treated with tetracycline when compared to EDTA group. However, EDTA was found to be much more effective as root conditioning agent because it enlarged the diameter of dentinal tubules more than that of tetracycline HCl. Conclusion: The results of in vitro study showed that both the agents are good root conditioning agents if applied in addition to periodontal therapy. However, further studies are required to establish the in vivo importance of EDTA and tetracycline HCL as root conditioners.

  9. Revisiting normal {sup 51}Cr-ethylenediaminetetraacetic acid clearance values in children

    Energy Technology Data Exchange (ETDEWEB)

    Piepsz, A.; Tondeur, M. [Department of Radioisotopes, CHU St Pierre, Brussels (Belgium); Ham, H. [Department of Nuclear Medicine, UZ Ghent, Ghent (Belgium)

    2006-12-15

    Normal {sup 51}Cr-ethylenediaminetetraacetic acid (EDTA) clearance values as a function of age were published a number of years ago. These values were based on data from children with a normal left to right ratio and a normal appearance on DMSA scintigraphy, despite the presence of an acute renal infection. At that time, the authors were unaware that hyperfiltration is a common phenomenon in patients with acute renal infection and that their normal values could have been significantly overestimated. The present work therefore aimed to re-appraise these normal values. In a first step, in order to verify the previous results, the same type of population was selected, namely patients with present or past urinary tract infection but normal images and a normal left to right ratio on DMSA scintigraphy. In a second step, the selection was based on patients who had had no recent urinary tract infection. In both series, a single blood sample method was used for the evaluation of {sup 51}Cr-EDTA clearance. In the first group of patients, the results obtained were almost identical to those previously published. In the second group of patients, the results were significantly lower: after 2 years of age, the mean GFR value was 104 ml/min/1.73 m{sup 2} (10th and 90th percentiles 81 and 135 ml/min/1.73 m{sup 2}, respectively), compared with 117 ml/min/1.73 m{sup 2} in the first group. The data of the second group are probably more representative of the true normal GFR values and can be applied to the entire paediatric population. (orig.)

  10. Intestinal permeability of 51Cr-labelled ethylenediaminetetraacetic acid in patients with Crohn's disease and their healthy relatives

    International Nuclear Information System (INIS)

    Ainsworth, M.; Eriksen, J.; Rasmussen, J.W.; Muckadell, O.B.S. de

    1989-01-01

    An increased intestinal permeability has been proposed as an aetiologic factor in Crohn's disease. The 24-h urinary excretion of 100 μCi 51 Cr-labelled ethylenediaminetetraacetic acid (EDTA) was used to test the permeability in 15 patients with Crohn's disease and in 20 healthy first-degree relatives, who were known to have a genetic predisposition to inflammatory bowel disease. Twenty-eight healthy persons not related to patients with inflammatory bowel disease served as control material. The 51 Cr-EDTA excretion of the relatives was not significantly higher than that of the controls, whereas patients with Crohn's disease had a significantly higher excretion than both the relatives and the controls. Among patients the increased excretion was found only if the small intestine was involved. It is concluded that 1) as a group, patient with Crohn's disease in the small intestine have an increased intestinal permeability, in contrast to their healthy relatives, who have a normal permeability; 2) a considerable overlap of the results of the 51 Cr-EDTA test was found between the groups studied, and the test is not suitable for evaluating individual patients; 3) the results do not support the hypothesis of an increase in intestinal permeability as an aetiologic factor in Crohn's disease. 29 refs

  11. Effect of EDTA and citric acid on phytoremediation of Cr- B[a]P-co-contaminated soil.

    Science.gov (United States)

    Chigbo, Chibuike; Batty, Lesley

    2013-12-01

    Polycyclic aromatic hydrocarbons and heavy metals in the environment are a concern, and their removal to acceptable level is required. Phytoremediation, the use of plants to treat contaminated soils, could be an interesting alternative to conventional remediation processes. This work evaluates the role of single and combined applications of chelates to single or mixed Cr + benzo[a]pyrene (B[a]P)-contaminated soil. Medicago sativa was grown in contaminated soil and was amended with 0.3 g citric acid, 0.146 g ethylenediaminetetraacetic acid (EDTA), or their combination for 60 days. The result shows that in Cr-contaminated soil, the application of EDTA + citric acid significantly (psoil. The soluble Cr concentration in single Cr or Cr + B[a]P-contaminated soil was enhanced with the amendment of all chelates; however, only the application of citric acid in Cr-contaminated soil (44 %) or EDTA and EDTA + citric acid in co-contaminated soil increased the removal of Cr from the soil (34 and 54 %, respectively). The dissipation of B[a]P in single B[a]P-contaminated soil was effective even without planting and amendment with chelates, while in co-contaminated soil, it was related to the application of either EDTA or EDTA + citric acid. This suggests that M. sativa with the help of chelates in single or co-contaminated soil can be effective in phytoextraction of Cr and promoting the biodegradation of B[a]P.

  12. Thermodynamics of mixed-ligand complex formation of mercury (II) ethylenediaminetetraacetate with amino acids in solution

    Energy Technology Data Exchange (ETDEWEB)

    Pyreu, Dmitrii, E-mail: pyreu@mail.ru [Department of Inorganic and Analytic Chemistry, Ivanovo State University, Ermak 39, Ivanovo 153025 (Russian Federation); Kozlovskii, Eugenii [Department of Inorganic and Analytic Chemistry, Ivanovo State University, Ermak 39, Ivanovo 153025 (Russian Federation); Gruzdev, Matvei; Kumeev, Roman [Institute of Solution Chemistry, Ivanovo (Russian Federation)

    2012-11-20

    Highlights: Black-Right-Pointing-Pointer Stable mixed ligand complexes of HgEdta with amino acids at physiological pH value. Black-Right-Pointing-Pointer The thermodynamic and NMR data evident the ambidentate coordination mode of arginine. Black-Right-Pointing-Pointer Participation of the guanidinic group of Arg in coordination process. Black-Right-Pointing-Pointer Binuclear complexes (HgEdta){sub 2}L with the bridging function of amino acid. - Abstract: The mixed-ligand complex formation in the systems Hg{sup 2+} - Edta{sup 4-} - L{sup -}(L = Arg, Orn, Ser) has been studied by means of calorimetry, pH-potentiometry and NMR spectroscopy in aqueous solution at 298.15 K and the ionic strength of I = 0.5 (KNO{sub 3}). The thermodynamic parameters of formation of the HgEdtaL, HgEdtaHL and (HgEdta){sub 2}L complexes have been determined. The most probable coordination mode for the complexone and the amino acid in the mixed-ligand complexes was discussed.

  13. Evaluation of smear layer removal and marginal adaptation of root canal sealer after final irrigation using ethylenediaminetetraacetic, peracetic, and etidronic acids with different concentrations.

    Science.gov (United States)

    Ulusoy, Özgür İlke; Zeyrek, Salev; Çelik, Bülent

    2017-07-01

    The purpose of this study was to investigate the effects of different irrigation solutions on the smear layer removal and marginal adaptation of a resin-based sealer to root canal dentine. A total of 152 instrumented roots were irrigated with the following irrigants: 9,18% etidronic acid (HEBP), 0.5, 1,2% peracetic acid (PAA), 17% ethylenediaminetetraacetic acid (EDTA), saline. The amount of smear layer was evaluated using scanning electron microscope (SEM) in seventy root samples. Eighty-two roots were filled with AH Plus and gutta-percha. Slices obtained from apical third of each specimen were viewed with SEM to assess marginal adaptation. Use of 9% and 18% HEBP resulted in more efficient smear layer removal in the apical third than the other chelators (p acid is a promising candidate for final irrigation of root canals. © 2017 Wiley Periodicals, Inc.

  14. Comparison of hematologic values in blood samples with lithium heparin or dipotassium ethylenediaminetetraacetic acid anticoagulants in Hispaniolan Amazon parrots (Amazona ventralis).

    Science.gov (United States)

    Guzman, David Sanchez-Migallon; Mitchell, Mark A; Gaunt, Stephen D; Beaufrère, Hugues; Tully, Thomas N

    2008-06-01

    Blood samples were collected from 20 Hispaniolan Amazon parrots (Amazona ventralis) and were divided into tubes that contained dipotassium ethylenediaminetetraacetic acid (K2EDTA) and lithium heparin. Complete blood cell counts were determined in each sample within 2 hours of collection. The level of agreement in results was moderate for plasma protein, packed cell volume (PCV), and leukocyte, monocyte, and lymphocyte counts between the anticoagulants. Plasma protein and PCV values were significantly lower in samples with lithium heparin than in those with K2EDTA, whereas lymphocyte numbers were significantly higher in lithium heparin samples than in K2EDTA samples. The level of agreement was good for the other cell types (heterophils, eosinophils, and basophils) when comparing the different anticoagulants. The poor level of agreement between anticoagulants with the increase in thrombocyte clumping in lithium heparin samples indicates that the use of lithium heparin as anticoagulant may affect thrombocyte count. No negative effects on morphology and staining of blood cells were apparent in smears from heparin samples compared with K2EDTA samples. Within the different values compared, the limits of agreement are small enough to be confident that lithium heparin can be used for routine CBC counts in a clinical setting. The use of the same anticoagulant should be recommended to follow trends within the same patient, especially when considering plasma protein concentration, PCV, and lymphocyte count.

  15. Comparative assessment of fluorosed and nonfluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after scaling and root planning and ethylenediaminetetraacetic acid root biomodification

    Directory of Open Access Journals (Sweden)

    Kharidi Laxman Vandana

    2014-01-01

    Full Text Available Background and Objectives: Fluorosis causes mineralization changes in the tooth and can lead to morphological alterations of fibroblasts. To evaluate the effect of fluorosis on periodontal healing, the initial step while healing such as, fibroblast attachment onto the root surface requires to be evaluated on the fluorosed and nonfluorosed tooth using nonfluorosed as well as fluorosed fibroblasts originated from the subjects influenced by high-water fluoride. Hence, the objective of the current study was to study and compare the attachment of nonfluorosed and fluorosed fibroblasts on the fluorosed and nonfluorosed root fragments. Materials and Methods: A total of 112 fluorosed and nonfluorosed, periodontally healthy and diseased tooth roots were obtained and allotted to eight groups : f0 luorosed healthy (FH and non-FH (NFH controls, fluorosed diseased (FD and non-FD (NFD controls, fluorosed and nonfluorosed teeth treated with scaling and root planning (SRP (FD SRP and NFD SRP and similar groups treated with SRP and ethylenediaminetetraacetic acid (EDTA (FD SRP + EDTA and NFD SRP + EDTA burnishing treatment with 24% EDTA gel for 2 min. After the respective treatment half of the root fragments in each group were incubated in the human periodontal ligament fibroblast cells obtained and cultured from freshly extracted FH and NFH human premolar tooth root. The nonfluorosed fibroblasts are elongated, flat cells thus they show increased attachment to root the surface. Results: When comparison was carried out between the attachment of fluorosed and nonfluorosed fibroblasts on NFD groups treated with scaling and EDTA, significant results were obtained with increased attachment seen on the group incubated with nonfluorosed fibroblasts (P = 0.029. While on comparison between the attachment of fluorosed and nonfluorosed fibroblasts on NFH group, NFD group treated with SRP and NFD group, no significant results were obtained (P > 0.05. On comparison between

  16. Effect of BioSentry 904 and ethylenediaminetetraacetic acid-tris disinfecting during incubation of chicken eggs on microbial levels and productivity of poultry.

    Science.gov (United States)

    Walker, S E; Sander, J E

    2004-01-01

    Proper sanitation practices and the use of efficacious disinfectants in a hatchery have an effect on chick quality. Aerosol bacterial counts, egg moisture loss, hatchability, chick quality, and broiler productivity were evaluated when egg surfaces were contaminated by immersion of each egg into a broth medium containing a field isolate of Pseudomonas aeruginosa and incubated with exposure to one of three disinfectant treatments administered by fine spray: distilled water, BioSentry 904 (904), and a 1:1 ratio of 904 and ethylenediaminetetraacetic acid (EDTA)-Tris. The aerosol bacteria levels were statistically greater on day 21 of incubation in the group treated with distilled water than in those receiving disinfectants. Overall hatch of fertile eggs and egg moisture loss were comparable among all three treatments. At 1 day of age, the chicks incubated with 904 had a statistically lower yolk sac contamination rate than those incubated with 904+EDTA-Tris or distilled water. The 2-wk mortality rates, body weights, feed conversion ratios, yolk sac weights, and yolk sac contamination rates were all similar among the three treatments.

  17. Relevance of EDTA carryover during blood collection.

    Science.gov (United States)

    Cadamuro, Janne; Felder, Thomas Klaus; Oberkofler, Hannes; Mrazek, Cornelia; Wiedemann, Helmut; Haschke-Becher, Elisabeth

    2015-07-01

    The order of draw is regarded as a preanalytical issue to prevent carryover of additives during blood collection. Our objective was to prove the theory of ethylenediaminetetraacetic acid (EDTA) carryover for a closed vacuum system and the influence of EDTA on concentrations of selected biomarkers. To test the carryover of EDTA, a blood collection with tripotassium EDTA (K3EDTA) and subsequent non-additive tubes was simulated using distilled water as substitute for blood. EDTA concentrations were measured by tandem mass spectrometry. Then we added increasing concentrations of EDTA to heparinized blood and measured routine biomarkers, thereby simulating a carryover of EDTA whole blood and pure EDTA, respectively. Additionally, we tested for EDTA contamination and biomarker alteration in samples collected from 10 healthy volunteers by a syringe with subsequent transfer into sample tubes. No EDTA contamination was detected in samples collected subsequent to a K3EDTA tube when adhering to guidelines of blood sampling. Magnesium, calcium, and potassium levels were altered by artificial K3EDTA whole-blood contamination as well as when adding 1 μL pure K3EDTA. Iron values were altered at EDTA concentrations of 4.4 mmol/L. All other parameters remained unaffected. A slight EDTA carryover was observed in syringe collection and subsequent transfer into EDTA and heparin tubes, however, without any biomarker alteration. An EDTA carryover during blood collection using a closed vacuum system is highly unlikely. Even if carryover of EDTA whole blood occurs, an absolute volume larger than 10 μL would be necessary to alter test results. However, contamination of samples with preloaded pure K3EDTA solution by severe neglect of current recommendations in blood collection may significantly alter testing results.

  18. Lipid oxidation in fish oil enriched mayonnaise : Calcium disodium ethylenediaminetetraacetate, but not gallic acid, strongly inhibited oxidative deterioration

    DEFF Research Database (Denmark)

    Jacobsen, Charlotte; Hartvigsen, Karsten; Thomsen, Mikael Holm

    2001-01-01

    The antioxidative effects of gallic acid, EDTA, and extra emulsifier Panodan DATEM TR in mayonnaise enriched with 16% fish oil were investigated. EDTA reduced the formation of free radicals, lipid hydroperoxides, volatiles, and fishy and rancid off-flavors. The antioxidative effect of EDTA...... acid may therefore promote the decomposition of lipid hydroperoxides to volatile oxidation products. Addition of extra emulsifier reduced the lipid hydroperoxide levels but did not influence the level of free radicals or the oxidative flavor deterioration in mayonnaisse; however, it appeared to alter...

  19. EDTA-S: A novel root conditioning agent

    Directory of Open Access Journals (Sweden)

    S Srirangarajan

    2012-01-01

    Full Text Available Background: To evaluate the efficacy of 15% ethylenediaminetetraacetic acid (EDTA-S (EDTA with soft soap preparation for the removal of smear layer at human root surfaces. Materials and Methods: Twenty teeth indicated for extraction due to periodontal disease were sectioned using high speed cylindrical bur under copious irrigation. The root surfaces were instrumented with Gracey 7-8 curette (Hu-Friedy, 12 times to induce an "experimental smear layer". Following root planning, the root surface was cut using diamond disc and separated from the crown. Samples were randomly distributed into five groups. One group was control, saline and test groups were EDTA 15% alone, by active and passive applications (groups 2 and 3, and EDTA 15%+soft soap, by active and passive applications (groups 4 and 5. Specimens were then subjected to scanning electron microscope study. Smear layer removal was evaluated according to Sampaio et al., index. Results: EDTA-S removed the smear layer better than plain EDTA and the control group, while active application of the root conditioning agent had significant difference than the passive application of the agent. Conclusion: EDTA-S has favorable benefits over EDTA alone, and active application is better in comparison with passive application of root conditioning agent. Clinical Relevance: Removal of smear layer has been considered as an important step in periodontal regenerative therapy. Scaling and root planning alone with saline irrigation does not remove the smear layer. EDTA is a commonly used root conditioning agent in periodontal therapy. The addition of a detergent to EDTA proved to remove smear layer more efficiently than EDTA alone.

  20. Decontamination effectiveness of mixtures of citric acid, oxalic acid and EDTA

    International Nuclear Information System (INIS)

    Speranzini, R.A.

    1990-01-01

    An experimental study of the decontamination effectiveness of citric acid, oxalic acid and EDTA mixtures was conducted to assess whether oxalic acid could be removed from decontamination solutions to minimize corrosion. In loop experiments, radioactive specimens from two boiling water reactors and one pressurized water reactor were suspended in solutions of single acids or in mixtures of reagents at total reagent concentrations of less than 0.1 wt% under conditions similar to those used to decontaminate reactor systems. Rate constants for dissolution of oxides and decontamination factors were measured. Based on the results, it was concluded that under certain conditions, oxalic acid was the most effective reagent for the dissolution of oxides. It was also found, however, that conditions under which effective dissolution occurred in solutions of oxalic acid and/or citric acid were difficult to define and control. EDTA was found to be an effective reagent for dissolution of oxides such that rates of dissolution in EDTA containing solutions at 117 degrees Celsius were comparable to rates in oxalic acid containing solutions. At 90 degrees Celsius, EDTA acted synergistically with oxalic acid such that the rate of dissolution of oxides in citric-acid/oxalic-acid/EDTA solutions was higher than in citric-acid/EDTA solutions. The rates of dissolution of oxides were significantly reduced when 60 mg/kg of ferric ion was added to the citric-acid/oxalic-acid, citric-acid/EDTA and citric-acid/oxalic-acid/EDTA solutions. It was concluded that effective decontaminations of BWR and PWR systems could be achieved with mixtures of citric acid and EDTA

  1. Characterisation of thorium-ethylenediaminetetraacetic acid and thorium-nitrilotriacetic acid species by electrospray ionisation-mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Cartwright, Andrew J. [Biogeochemistry and Environmental Analytical Chemistry Group, School of Earth, Ocean and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); May, Colin C. [Biogeochemistry and Environmental Analytical Chemistry Group, School of Earth, Ocean and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Worsfold, Paul J. [Biogeochemistry and Environmental Analytical Chemistry Group, School of Earth, Ocean and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Keith-Roach, Miranda J. [Biogeochemistry and Environmental Analytical Chemistry Group, School of Earth, Ocean and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom)]. E-mail: mkeith-roach@plymouth.ac.uk

    2007-05-02

    Electrospray ionisation-mass spectrometry (ESI-MS) has been used to investigate the speciation of Th in the presence of two common organic complexing agents found in radioactive wastes, EDTA and NTA. These ligands may enhance radionuclide migration from nuclear wastes and contaminated land, and characterisation of the complexes formed will improve our understanding of their environmental mobility. When acetic acid and ammonia were used to adjust the pH, the dominant Th-EDTA species changed from the mixed ligand [ThEDTAac]{sup -} complex to [ThEDTAOH]{sup -} and [ThEDTA(OH){sub 2}ac]{sup 3-} as the pH increased, with all species co-existing, to some extent, over the pH range (2.5-10.8). A previously suggested Th-NTA species, [ThNTA{sub 2}]{sup 2-}, dominates from pH 6.0-8.5 but at high pH (10.0-10.8) NTA was not able to solubilise Th to a measurable extent. In the presence of both EDTA and NTA, Th formed a mixed ligand complex, [ThEDTANTA]{sup 3-} which has also been characterised in independent experiments. The identification of the importance of [ThNTA{sub 2}]{sup 2-}, [ThEDTANTA]{sup 3-} and [ThEDTA(OH){sub 2}]{sup 2-} (with an additional acetate ligand) highlights that these species are not included in current speciation databases and thus may impact on predictions of the environmental mobility of Th. ESI-MS has therefore been used to identify and confirm fundamentally important Th complexes. It has proved to be a useful tool for elucidating radionuclide speciation, adding to our knowledge of Th geochemistry and providing a means of critically examining existing stability constant data.

  2. Characterisation of thorium-ethylenediaminetetraacetic acid and thorium-nitrilotriacetic acid species by electrospray ionisation-mass spectrometry

    International Nuclear Information System (INIS)

    Cartwright, Andrew J.; May, Colin C.; Worsfold, Paul J.; Keith-Roach, Miranda J.

    2007-01-01

    Electrospray ionisation-mass spectrometry (ESI-MS) has been used to investigate the speciation of Th in the presence of two common organic complexing agents found in radioactive wastes, EDTA and NTA. These ligands may enhance radionuclide migration from nuclear wastes and contaminated land, and characterisation of the complexes formed will improve our understanding of their environmental mobility. When acetic acid and ammonia were used to adjust the pH, the dominant Th-EDTA species changed from the mixed ligand [ThEDTAac] - complex to [ThEDTAOH] - and [ThEDTA(OH) 2 ac] 3- as the pH increased, with all species co-existing, to some extent, over the pH range (2.5-10.8). A previously suggested Th-NTA species, [ThNTA 2 ] 2- , dominates from pH 6.0-8.5 but at high pH (10.0-10.8) NTA was not able to solubilise Th to a measurable extent. In the presence of both EDTA and NTA, Th formed a mixed ligand complex, [ThEDTANTA] 3- which has also been characterised in independent experiments. The identification of the importance of [ThNTA 2 ] 2- , [ThEDTANTA] 3- and [ThEDTA(OH) 2 ] 2- (with an additional acetate ligand) highlights that these species are not included in current speciation databases and thus may impact on predictions of the environmental mobility of Th. ESI-MS has therefore been used to identify and confirm fundamentally important Th complexes. It has proved to be a useful tool for elucidating radionuclide speciation, adding to our knowledge of Th geochemistry and providing a means of critically examining existing stability constant data

  3. Reaction of single-standard DNA with hydroxyl radical generated by iron(II)-ethylenediaminetetraacetic acid

    International Nuclear Information System (INIS)

    Prigodich, R.V.; Martin, C.T.

    1990-01-01

    This study demonstrates that the reaction of Fe(II)-EDTA and hydrogen peroxide with the single-stranded nucleic acids d(pT) 70 and a 29-base sequence containing a mixture of bases results in substantial damage which is not directly detected by gel electrophoresis. Cleavage of the DNA sugar backbone is enhanced significantly after the samples are incubated at 90 degree C in the presence of piperidine. The latter reaction is used in traditional Maxam-Gilbert DNA sequencing to detect base damage, and the current results are consistent with reaction of the hydroxyl radical with the bases in single-stranded DNA (although reaction with sugar may also produce adducts that are uncleaved but labile to cleavage by piperidine). We the authors propose that hydroxyl radicals may react preferentially with the nucleic acid bases in ssDNA and that reaction of the sugars in dsDNA is dominant because the bases are sequestered within the double helix. These results have implications both for the study of single-stranded DNA binding protein binding sites and for the interpretation of experiments using the hydroxyl radical to probe DNA structure or to footprint double-stranded DNA binding protein binding sites

  4. Effect of EDTA and phosphoric Acid pretreatment on the bonding effectiveness of self-etch adhesives to ground enamel.

    Science.gov (United States)

    Ibrahim, Ihab M; Elkassas, Dina W; Yousry, Mai M

    2010-10-01

    This in vitro study determined the effect of enamel pretreatment with phosphoric acid and ethylenediaminetetraacetic acid (EDTA) on the bond strength of strong, intermediary strong, and mild self-etching adhesive systems. Ninety sound human premolars were used. Resin composite cylinders were bonded to flat ground enamel surfaces using three self-etching adhesive systems: strong Adper Prompt L-Pop (pH=0.9-1.0), intermediary strong AdheSE (pH=1.6-1.7), and mild Frog (pH=2). Adhesive systems were applied either according to manufacturer instructions (control) or after pretreatment with either phosphoric acid or EDTA (n=10). After 24 hours, shear bond strength was tested using a universal testing machine at a cross-head speed of 0.5 mm/minute. Ultra-morphological characterization of the surface topography and resin/enamel interfaces as well as representative fractured enamel specimens were examined using scanning electron microscopy (SEM). Neither surface pretreatment statistically increased the mean shear bond strength values of either the strong or the intermediary strong self-etching adhesive systems. However, phosphoric acid pretreatment significantly increased the mean shear bond strength values of the mild self-etching adhesive system. SEM examination of enamel surface topography showed that phosphoric acid pretreatment deepened the same etching pattern of the strong and intermediary strong adhesive systems but converted the irregular etching pattern of the mild self-etching adhesive system to a regular etching pattern. SEM examination of the resin/enamel interface revealed that deepening of the etching pattern was consistent with increase in the length of resin tags. EDTA pretreatment had a negligible effect on ultra-morphological features. Use of phosphoric acid pretreatment can be beneficial with mild self-etching adhesive systems for bonding to enamel.

  5. Nisin, rosemary, and ethylenediaminetetraacetic acid affect the growth of Listeria monocytogenes on ready-to-eat turkey ham stored at four degrees Celsius for sixty-three days.

    Science.gov (United States)

    Ruiz, A; Williams, S K; Djeri, N; Hinton, A; Rodrick, G E

    2009-08-01

    The objectives of this study were to determine the anti-Listeria and general antimicrobial properties of nisin, rosemary, and EDTA alone and in combination on Listeria monocytogenes inoculated on ready-to-eat vacuum-packaged diced turkey ham and to ascertain the effects of the treatments on pH and objective color. The turkey hams were cut into 0.5-cm pieces, inoculated with a L. monocytogenes cocktail containing 5 strains of the bacterium, and treated with either no treatment and no inoculum (negative control), inoculum only (positive control), 0.5% nisin, 20 mM EDTA, 1% rosemary, 0.5% nisin + 20 mM EDTA, 0.5% nisin + 1% rosemary, 0.5% nisin + 20 mM EDTA + 1% rosemary, or 20 mM EDTA + 1% rosemary. All samples were vacuum-packaged, stored for 63 d at 4 degrees C +/- 1 degrees C, and analyzed at 1-wk intervals for total aerobes, L. monocytogenes, lactic acid organisms, pH, and objective color. Nisin, nisin with rosemary, nisin with EDTA, and nisin with rosemary and EDTA treatments reduced (P hams treated with nisin. The EDTA and rosemary treatments alone and in combination were ineffective in inhibiting growth of L. monocytogenes. Although none of the treatments completely eliminated L. monocytogenes, the results indicated that ready-to-eat turkey ham can have significantly decreased L. monocytogenes when treated with nisin alone or in combination with rosemary or EDTA, or both.

  6. EDTA sample contamination is common and often undetected, putting patients at unnecessary risk of harm.

    Science.gov (United States)

    Sharratt, C L; Gilbert, C J; Cornes, M C; Ford, C; Gama, R

    2009-08-01

    Potassium ethylenediaminetetraacetic acid (EDTA) is a sample tube anticoagulant used for many laboratory analyses. Gross potassium EDTA contamination of blood samples is easily recognised by marked hyperkalaemia and hypocalcaemia. However, subtle contamination is a relatively common, often unrecognised erroneous cause of spurious hyperkalaemia. Potassium EDTA contamination may also cause hypomagnesaemia and hypozincaemia. There are, however, no data on the prevalence of EDTA contamination as a cause of hypocalcaemia, hypomagnesaemia and hypozincaemia. Following a recent service evaluation, we measure EDTA in serum samples from patients with unexplained hyperkalaemia (serum potassium > 6.0 mmol/l). In addition, over a 1-month period EDTA concentrations were measured in hypocalcaemic (serum adjusted calcium samples. Ethylenediaminetetraacetic acid contamination was detected in 31 samples, nine of which were detected by our routine screening programme. The remaining 22 samples represented 14.3% (19/133) of hypocalcaemic samples, 4.8% (5/104) of hypomagnesaemic samples and 1.4% (2/139) of hypozincaemic samples. A total of 25/31 (80.6%) of patients were re-bled, of which 23/25 (92%) results normalised. Factitious hyperkalaemia, hypocalcaemia and hypomagnesaemia caused by potassium EDTA contamination in our studies are relatively common, and if unrecognised may adversely affect patient care and waste scarce healthcare resources. Correct order of draw of blood samples, improved education and routine laboratory screening of EDTA are necessary to prevent and identify EDTA contamination.

  7. K2-EDTA and K3-EDTA Greiner Tubes for HbA1c Measurement.

    Science.gov (United States)

    Vrtaric, Alen; Filipi, Petra; Hemar, Marina; Nikolac, Nora; Simundic, Ana-Maria

    2016-02-01

    To determine whether K2-ethylenediaminetetraacetic acid (EDTA) and K3-EDTA Greiner tubes could be used interchangeably for glycosylated hemoglobin, type A1C (HbA1c) measurement via the Abbott Laboratories ARCHITECT chemiluminescent microparticle HbA1c assay on the ARCHITECT i2000SR immunoanalyzer at our university hospital. We drew blood from a total of 45 outpatients into plastic Greiner Vacuette tubes, some of which were lined with K2-EDTA and others with K3-EDTA anticoagulant. Data are presented as median and interquartile range values. We used the Wilcoxon test and Passing-Bablok regression for tube comparison. For K2-EDTA tubes median HbA1c concentration was 54 mmol/mol (41 to 71 mmol/mol) and for K3-EDTA tubes 56 mmol/mol (43 to 69 mmol/mol). There was no statistically significant difference between K2-EDTA and K3-EDTA (bias= -1.29 mmol/mol; P = 0.24). Passing-Bablok regression showed that there is no constant and proportional error: y = -0.23 (95% CI[-3.52 to 0.69]) + 1.00( 95% CI[0.98 to 1.06]) x. In this study, we provide evidence for the lack of any clinically and statistically significant bias between K2-EDTA and K3-EDTA HbA1c measurements. Thus, Greiner tubes lined with K2-EDTA and those lined with K3-EDTA can safely be used interchangeably to measure HbA1c via the Abbott Laboratories ARCHITECT assay. © American Society for Clinical Pathology, 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  8. Noncovalent interaction of polyethylene glycol with copper complex of ethylenediaminetetraacetic acid and its application in constructing inorganic nanomaterials.

    Science.gov (United States)

    Pan, Shu Zhen; Song, Le Xin; Chen, Jie; Du, Fang Yun; Yang, Jing; Xia, Juan

    2011-10-21

    In this study, we try to answer a fundamental question: what is the consequence of the noncovalent interaction between a polymer and a coordination compound? Here, polyethylene glycol (PEG-4000, PEG-b) and copper complex of ethylenediaminetetraacetic acid (H(2)CuY) were employed to solve this problem. A novel adduct (CEP) between H(2)CuY and PEG-b was prepared. Our results indicated several interesting findings. First, the introduction of H(2)CuY had no effect on the stacking structure of PEG-b but led to a large change in surface structure of the polymer. Second, there was a significant difference (117 K) in the maximum degradation temperature between the PEG and the CEP, suggesting that the noncovalent interaction can drastically improve the thermal stability of the PEG. Third, sintering experiments showed that H(2)CuY and CEP produced completely different decomposition products. The former formed Cu crystals in nitrogen and CuO in air, but the latter generated Cu and CuCl crystals with good crystallinity, respectively. Finally, three independent measurements: viscosity, conductivity and nuclear magnetic resonance in solution, provided useful information and insights from both sides of the noncovalent interaction. Probable interaction mechanisms and interaction sites were proposed. We consider that the current research could create the foundation for a new understanding of how the noncovalent adduct interaction between a metallic complex and a polymer relates to the change in physical and chemical properties of the adducted components. This journal is © The Royal Society of Chemistry 2011

  9. Column leaching of chromium and nickel from a contaminated soil using EDTA and citric acid

    International Nuclear Information System (INIS)

    Jean-Soro, Liliane; Bordas, François; Bollinger, Jean-Claude

    2012-01-01

    This study investigates the column leaching of a soil contaminated mainly with Cr and Ni by using two chelants: citric acid (biodegradable) and EDTA (non-biodegradable) followed with water rinse. The chelants lead to Cr and Ni leaching, in addition to major elements (Ca, Fe, Mg, Al, Mn and Zn) showing the dissolution of soil mineral constituents. EDTA leaches more major elements and Ni than citric acid related to the respective stability of metal–chelant complexes; citric acid leaches more Cr than EDTA, certainly because of a substitution reaction with Cr(VI). In the case of alternating chelant/water applications, leaching occurs during the chelant applications, but also during water applications. In the case of chelant/water applications followed by continuous water application, both Cr and Ni leach over time. This increased mobility could be due to the residual chelant present in soil as well as to the dissolution/mobilization of mineral or organic soil fractions. - Highlights: ► Column leaching of an industrial soil contaminated with chromium and nickel. ► Citric acid or EDTA were used alternatively or followed with water rinse. ► Chelants lead to Cr and Ni leaching and the dissolution of soil mineral constituents. ► Leaching of these two metals proceeds continuously during water rinse. ► Chelants deeply impacted Cr and Ni mobility. - Citric acid or EDTA application deeply impact Cr and Ni mobility during column leaching of a contaminated soil.

  10. Recovery of manganese from manganese oxide ores in the EDTA solution

    Science.gov (United States)

    Zhang, Chao; Wang, Shuai; Cao, Zhan-fang; Zhong, Hong

    2018-04-01

    A new process has been experimentally and theoretically established for the recovery of manganese from manganese oxide ores, mainly including the reductive leaching of manganese by ethylenediaminetetraacetic acid (EDTA), EDTA recovery, and manganese electrolysis. The experimental conditions for this process were investigated. Moderate leaching environment by EDTA with the pH in the range of 5-6 is of benefit to leach manganese from some manganese oxide ores with high-content impurities, such as iron and aluminum. Most of EDTA can be recovered by acidification. A small amount of the residual EDTA in the electrolyte can prevent the generation of anode mud. In addition, trimanganese tetroxide (Mn3O4) can be obtained by the roasting of the EDTA-Mn crystallized product.

  11. Determination of ethylenediaminetetraacetic acid in nuclear waste by high-performance liquid chromatography coupled with electrospray mass spectrometry.

    Science.gov (United States)

    du Bois de Maquillé, Laurence; Renaudin, Laetitia; Goutelard, Florence; Jardy, Alain; Vial, Jérôme; Thiébaut, Didier

    2013-02-08

    EDTA is a chelating agent that has been used in decontamination processes. Its quantification is required for nuclear waste management because it affects the mobility of radionuclides and metals in environment and, thus, can harm the safety of the storage. Ion-pair chromatography coupled with electrospray mass spectrometry detection is a convenient method for quantitative analysis of EDTA but EDTA should be present as a single anionic chelate form. However, radioactive liquid wastes contain high concentrations of heavy metals and salts and consequently, EDTA is present as several chelates. Speciation studies were carried out to choose a metal cation to be added in excess to the solution to obtain a major chelate form. Fe is the predominant cation and Fe(III)-EDTA is thermodynamically favored but these speciation studies showed that ferric hydroxide precipitated above pH 2. Consequently, it was not possible to quantify EDTA as Fe(III)-EDTA complex. Therefore, Ni(2+) was chosen but its use implied pretreatment with a base of the solution to eliminate Fe. Deuterated EDTA was used as tracer in order to validate the whole procedure, from the treatment with a base to the final analysis by HPLC-ESI-MS. This analytical method was successfully applied for EDTA quantification in two real effluents resulting from a nuclear liquid waste process. A recovery rate between 60 and 80% was obtained. The limit of detection of this method was determined at 34×10(-9)mol L(-1). Copyright © 2012 Elsevier B.V. All rights reserved.

  12. Antimony(3) ethylenediaminetetraacetate complexes with single- and doubly charged cations

    International Nuclear Information System (INIS)

    Davidovich, R.L.; Logvinova, V.B.; Kajdalova, T.A.

    1998-01-01

    The antimony(3) ethylenediaminetetraacetate complexes with alkaline and bivalent metals cations of the M + Sb(Edta) · H 2 O (M + = K, Rb, Cs, NH 4 ), M 2+ [Sb(Edta)] 2 · 8H 2 O (M 2+ = Mg, Ca, Sr, Co, Cd) composition are synthesized. Roentgenographic and IR-spectroscopic characteristics of the synthesized substances are determined. Two groups of the isostructural compounds: M + Sb(Edta) · H 2 O (M + = K, Rb, NH 4 ) and M 2+ [Sb(Edta)] 2 · 8H 2 O (M 2+ = Mg, Ca, Sr, Mn, Co, Cd) are established [ru

  13. Determination of ethylenediaminetetraacetic acid in sea water by solid-phase extraction and high-performance liquid chromatography.

    Science.gov (United States)

    Kemmei, Tomoko; Kodama, Shuji; Fujishima, Hironori; Yamamoto, Atsushi; Inoue, Yoshinori; Hayakawa, Kazuichi

    2012-01-04

    The chelating agent EDTA is widely used, and as a result is showing up widely in the aquatic environment. Here we describe a preconcentration procedure for measuring EDTA concentration in sea water samples by HPLC. The procedure consists of forming an Fe(III) complex followed by solid-phase extraction using an activated carbon cartridge. After the preconcentration, EDTA was quantified by HPLC with ultraviolet detection (260 nm). The enrichment permitted the determination of EDTA at concentrations as low as 1 nM. Good recoveries were obtained for both brackish and full-strength sea water with high repeatability (RSD<6%). The method was applied to sea water samples taken from near the mouth of the Oyabe River in Japan. Copyright © 2011 Elsevier B.V. All rights reserved.

  14. Effects of salinity and ethylenediamine tetra acetic acid (edta) on the ...

    African Journals Online (AJOL)

    In this study, the effects of the combined treatment of salinity and ethylenediamine tetra acetic acid (EDTA) on the germination of tomato seeds in Petri-dishes were compared to sole salinity. The treatments consisted of seven concentrations of sodium chloride (NaCL): 0 (control), 10, 50, 100, 250, 500 and 1000 mM.

  15. Effect of citric acid and EDTA on chromium and nickel uptake and translocation by Datura innoxia

    International Nuclear Information System (INIS)

    Jean, Liliane; Bordas, Francois; Gautier-Moussard, Cecile; Vernay, Philippe; Hitmi, Adnane; Bollinger, Jean-Claude

    2008-01-01

    EDTA and citric acid were tested to solubilize metals and enhance their uptake by Datura innoxia, chosen because of its ability to accumulate and tolerate metals. Two application modes were used on an industrial soil contaminated mainly by Cr and Ni. The results showed that citric acid was the most effective at increasing the uptake of Cr and EDTA for Ni. These results are consistent with the effectiveness of both chelants in solubilizing metals from the soil. The translocation factor (TF) of Ni was 1.6- and 6.7-fold higher than the control, respectively, for one and two applications of 1 mmol kg -1 EDTA. After two applications of 5 and 10 mmol kg -1 citric acid, the TF of Cr increased 2- and 3.5-fold relative to the control. Whatever the concentration, the application of EDTA modified the plant physiology significantly. For citric acid this was only observed with the highest dose (10 mmol kg -1 ). - Chelant effectiveness in increasing chromium and nickel uptake by Datura innoxia is the result of the increase in translocation versus negative effect on plant physiology

  16. Effects of EDTA and low molecular weight organic acids on soil solution properties of a heavy metal polluted soil.

    Science.gov (United States)

    Wu, L H; Luo, Y M; Christie, P; Wong, M H

    2003-02-01

    A pot experiment was conducted to study the effects of EDTA and low molecular weight organic acids (LMWOA) on the pH, total organic carbon (TOC) and heavy metals in the soil solution in the rhizosphere of Brassica juncea grown in a paddy soil contaminated with Cu, Zn, Pb and Cd. The results show that EDTA and LMWOA have no effect on the soil solution pH. EDTA addition significantly increased the TOC concentrations in the soil solution. The TOC concentrations in treatments with EDTA were significantly higher than those in treatments with LMWOA. Adding 3 mmol kg(-1) EDTA to the soil markedly increased the total concentrations of Cu, Zn, Pb and Cd in the soil solution. Compared to EDTA, LMWOA had a very small effect on the metal concentrations. Total concentrations in the soil solution followed the sequence: EDTA > citric acid (CA) approximately oxalic acid (OA) approximately malic acid (MA) for Cu and Pb; EDTA > MA > CA approximately OA for Zn; and EDTA > MA > CA > OA for Cd. The labile concentrations of Cu, Zn, Pb and Cd showed similar trends to the total concentrations.

  17. [Evaluation of compounding EDTA and citric acid on remediation of heavy metals contaminated soil].

    Science.gov (United States)

    Yin, Xue; Chen, Jia-Jun; Cai, Wen-Min

    2014-08-01

    As commonly used eluents, Na2EDTA (EDTA) and citric acid (CA) have been widely applied in remediation of soil contaminated by heavy metals. In order to evaluate the removal of arsenic, cadmium, copper, and lead in the contaminated soil collected in a chemical plant by compounding EDTA and CA, a series of stirring experiments were conducted. Furthermore, the changes in speciation distribution of heavy metals before and after washing were studied. The results showed that, adopting the optimal molar ratio of EDTA/CA (1:1), when the pH of the solution was 3, the stirring time was 30 min, the stirring rate was 150 r x min(-1) and the L/S was 5:1, the removal rates of arsenic, cadmium, copper and lead could reach 11.72%, 43.39%, 24.36% and 27.17%, respectively. And it was found that after washing, for arsenic and copper, the content of acid dissolved fraction rose which increased the percentage of available contents. Fe-Mn oxide fraction mainly contributed to the removal of copper. As for cadmium, the percentages of acid dissolved fraction, Fe-Mn oxide fraction and organic fraction also decreased. In practical projects, speciation changes would pose certain environmental risk after soil washing, which should be taken into consideration.

  18. Titration of thorium and rare earths with ethylenediaminetetraacetic acid using semimethylthymol blue by visual end-point indication

    International Nuclear Information System (INIS)

    Hafez, M.A.H.; Kenawy, I.M.M.; Ramadan, M.A.M.

    1994-01-01

    The precision and accuracy attainable in direct complexometric titrations of Thsup(4+) consecutively with either lighter (La 3+ , Nd 3+ , Sm 3+ , Eu 3+ or Gd 3+ ) or heavier lanthanides (Dy 3+ ) in different proportions using Semimethylthymol Blue (SMTB) as a metallochromic indicator and disodium dihydrogen ethylenediaminetetraacetate were studied. Thorium (IV) was titrated at pH 2, the Ph was adjusted to 5.5-6.0 by adding hexamethylenetetramine (hexamine) buffer and acetylacetone-acetone solution and La 3+ (or Nd 3+ , Sm 3+ , Eu 3+ , Gd 3+ or Dy 3+ ) was then titrated. A comparison of the indicators SMTB and Methylthymol Blue (MTB) for successive titrations of Th 4+ and any of the rare earth ions was carried out. The proposed titration method was applied successfully to some naturally occurring ores and minerals containing thorium and some lanthanides and the results were satisfactory. (Author)

  19. Influence of FeEDDS, FeEDTA, FeDTPA, FeEDDHA, and FeSO4 on Marigold Growth and Nutrition, and Substrate and Runoff Chemistry

    Science.gov (United States)

    Objectives of the study were to determine effects of Fe source on plant growth, plant nutrition, substrate chemistry and runoff chemistry. Iron source (FS) treatments consisted of Fe-aminopolycarboxylic acid (APCA) complexones iron ethylenediaminetetraacetic acid (FeEDTA), iron [S, S']-ethylenediam...

  20. Uranium Leaching from Contaminated Soil Utilizing Rhamnolipid, EDTA, and Citric Acid

    Directory of Open Access Journals (Sweden)

    Sara Asselin

    2014-01-01

    Full Text Available Biosurfactants have recently gained attention as “green” agents that can be used to enhance the remediation of heavy metals and some organic matter in contaminated soils. The overall objective of this paper was to investigate rhamnolipid, a microbial produced biosurfactant, and its ability to leach uranium present in contaminated soil from an abandoned mine site. Soil samples were collected from two locations in northern Arizona: Cameron (site of open pit mining and Leupp (control—no mining. The approach taken was to first determine the total uranium content in each soil using a hydrofluoric acid digestion, then comparing the amount of metal removed by rhamnolipid to other chelating agents EDTA and citric acid, and finally determining the amount of soluble metal in the soil matrix using a sequential extraction. Results suggested a complex system for metal removal from soil utilizing rhamnolipid. It was determined that rhamnolipid at a concentration of 150 μM was as effective as EDTA but not as effective as citric acid for the removal of soluble uranium. However, the rhamnolipid was only slightly better at removing uranium from the mining soil compared to a purified water control. Overall, this study demonstrated that rhamnolipid ability to remove uranium from contaminated soil is comparable to EDTA and to a lesser extent citric acid, but, for the soils investigated, it is not significantly better than a simple water wash.

  1. Formation of nitridotechnetium(VI) μ-oxo dimer complexes with EDTA and EDDA

    International Nuclear Information System (INIS)

    Takayama, T.; Kani, Y.; Sekine, T.; Kudo, H.; Yoshihara, K.

    1995-01-01

    Reactions of [ 99 TcNCl 4 ] - with ethylenediaminetetraacetic acid 4 (ETDA) and ethylenediamine-N,N'-diacetic acid (EDDA) in a mixture of water and acetone gave Tc VI N-EDTA and Tc VI N-EDTA complexes. The infrared spectra of both reaction products showed the existence of the Tc≡N and C=O groups. The elemental analysis indicated the 1:1 TcN-ligand ratio in the EDTA and EDDA complexes. Electrophoresis showed that the Tc VI -EDTA complex was an anionic species in a perchlorate solution. For the Tc VI N-EDDA complex, neutral and anionic species were formed, depending on the pH of the solution. Formation of the μ-oxo dimer complexes was suggested from the UV-Vis absorption spectra. (author) 11 refs.; 4 figs.; 1 tab

  2. EDTA assisted synthesis of hydroxyapatite nanoparticles for electrochemical sensing of uric acid

    Energy Technology Data Exchange (ETDEWEB)

    Kanchana, P.; Sekar, C., E-mail: Sekar2025@gmail.com

    2014-09-01

    Hydroxyapatite nanoparticles have been synthesized using EDTA as organic modifier by a simple microwave irradiation method and its application for the selective determination of uric acid (UA) has been demonstrated. Electrochemical behavior of uric acid at HA nanoparticle modified glassy carbon electrode (E-HA/GCE) has been investigated by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), linear sweep voltammetry (LSV) and amperometry. The E-HA modified electrode exhibits efficient electrochemical activity towards uric acid sensing without requiring enzyme or electron mediator. Amperometry studies revealed that the fabricated electrode has excellent sensitivity for uric acid with the lowest detection limit of 142 nM over a wide concentration range from 1 × 10{sup −7} to 3 × 10{sup −5} M. Moreover, the studied E-HA modified GC electrode exhibits a good reproducibility and long-term stability and an admirable selectivity towards the determination of UA even in the presence of potential interferents. The analytical performance of this sensor was evaluated for the detection of uric acid in human urine and blood serum samples. - Highlights: • EDTA- hydroxyapatite (HA) nanoparticles have been synthesized by microwave irradiation method. • A novel amperometric Uric Acid biosensor has been fabricated using E-HA/GCE. • The fabricated sensor exhibits a wide linear range, good stability and high reproducibility. • The sensor was applied for the detection of UA in human blood serum and urine.

  3. EDTA assisted synthesis of hydroxyapatite nanoparticles for electrochemical sensing of uric acid

    International Nuclear Information System (INIS)

    Kanchana, P.; Sekar, C.

    2014-01-01

    Hydroxyapatite nanoparticles have been synthesized using EDTA as organic modifier by a simple microwave irradiation method and its application for the selective determination of uric acid (UA) has been demonstrated. Electrochemical behavior of uric acid at HA nanoparticle modified glassy carbon electrode (E-HA/GCE) has been investigated by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), linear sweep voltammetry (LSV) and amperometry. The E-HA modified electrode exhibits efficient electrochemical activity towards uric acid sensing without requiring enzyme or electron mediator. Amperometry studies revealed that the fabricated electrode has excellent sensitivity for uric acid with the lowest detection limit of 142 nM over a wide concentration range from 1 × 10 −7 to 3 × 10 −5 M. Moreover, the studied E-HA modified GC electrode exhibits a good reproducibility and long-term stability and an admirable selectivity towards the determination of UA even in the presence of potential interferents. The analytical performance of this sensor was evaluated for the detection of uric acid in human urine and blood serum samples. - Highlights: • EDTA- hydroxyapatite (HA) nanoparticles have been synthesized by microwave irradiation method. • A novel amperometric Uric Acid biosensor has been fabricated using E-HA/GCE. • The fabricated sensor exhibits a wide linear range, good stability and high reproducibility. • The sensor was applied for the detection of UA in human blood serum and urine

  4. Comparison of EDTA- and citric acid-enhanced phytoextraction of heavy metals in artificially metal contaminated soil by Typha angustifolia.

    Science.gov (United States)

    Muhammad, Dawood; Chen, Fei; Zhao, Jing; Zhang, Guoping; Wu, Feibo

    2009-08-01

    A pot experiment was conducted to study the performance of EDTA and citric acid (CA) addition in improving phytoextraction of Cd, Cu, Pb, and Cr from artificially contaminated soil by T. angustifolia. T. angustifolia showed the remarkable resistance to heavy metal toxicity with no visual toxic symptom including chlorosis and necrosis when exposed to metal stress. EDTA-addition significantly reduced plant height and biomass, compared with the control, and stunted plant growth, while 2.5 and 5 mM CA addition induced significant increases in root dry weight. EDTA, and 5 and 10 mM CA significantly increased shoot Cd, Pb, and Cr concentrations compared with the control, with EDTA being more effective. At final harvest, the highest shoot Cd, Cr, and Pb concentrations were recorded in the treatment of 5 mM EDTA addition, while maximal root Pb concentration was found at the 2.5 mM CA treatment. However, shoot Cd accumulation in the 10 mM CA treatment was 36.9% higher than that in 2.5 mM EDTA, and similar with that in 10 mM EDTA. Shoot Pb accumulation was lower in 10 mM CA than that in EDTA treatments. Further, root Cd, Cu, and Pb accumulation of CA treatments and shoot Cr accumulation in 5 or 10 mM CA treatments were markedly higher than that of control and EDTA treatments. The results also showed that EDTA dramatically increased the dissolution of Cu, Cr, Pb, and Cd in soil, while CA addition had less effect on water-soluble Cu, Cr, and Cd, and no effect on Pb levels. It is suggested that CA can be a good chelator candidate for T. angustifolia used for environmentally safe phytoextraction of Cd and Cr in soils.

  5. Enhanced phytoextraction: II. Effect of EDTA and citric acid on heavy metal uptake by Helianthus annuus from a calcareous soil.

    Science.gov (United States)

    Lesage, E; Meers, E; Vervaeke, P; Lamsal, S; Hopgood, M; Tack, F M G; Verloo, M G

    2005-01-01

    High biomass producing plant species, such as Helianthus annuus, have potential for removing large amounts of trace metals by harvesting the aboveground biomass if sufficient metal concentrations in their biomass can be achieved However, the low bioavailability of heavy metals in soils and the limited translocation of heavy metals to the shoots by most high biomass producing plant species limit the efficiency of the phytoextraction process. Amendment of a contaminated soil with ethylene diamine tetraacetic acid (EDTA) or citric acid increases soluble heavy metal concentrations, potentially rendering them more available for plant uptake. This article discusses the effects of EDTA and citric acid on the uptake of heavy metals and translocation to aboveground harvestable plant parts in Helianthus annuus. EDTA was included in the research for comparison purposes in our quest for less persistent alternatives, suitable for enhanced phytoextraction. Plants were grown in a calcareous soil moderately contaminated with Cu, Pb, Zn, and Cd and treated with increasing concentrations of EDTA (0.1, 1, 3, 5, 7, and 10 mmol kg(-1) soil) or citric acid (0.01, 0.05, 0.25, 0.442, and 0.5 mol kg(-1) soil). Heavy metal concentrations in harvested shoots increased with EDTA concentration but the actual amount of phytoextracted heavy metals decreased at high EDTA concentrations, due to severe growth depression. Helianthus annuus suffered heavy metal stress due to the significantly increased bioavailable metal fraction in the soil. The rapid mineralization of citric acid and the high buffering capacity of the soil made citric acid inefficient in increasing the phytoextracted amounts of heavy metals. Treatments that did not exceed the buffering capacity of the soil (heavy metal concentrations. Treatments with high concentrations resulted in a dissolution of the carbonates and compaction of the soil. These physicochemical changes caused growth depression of Helianthus annuus. EDTA and citric

  6. Complexometric determination: Part I - EDTA and complex formation with the Cu2+ ion

    Directory of Open Access Journals (Sweden)

    Rajković Miloš B.

    2002-01-01

    Full Text Available Compounds forming very stable complexes - chelates, have a wide field of application in analytical chemistry. The most famous group of these compounds are complexons. Complexons represent organic polyaminocarbonic acids as for example ethylenediaminetetraacetic acid (EDTA and its salts. The EDTA molecule has six coordinative sites. It is a hexadentate ligands i.e. it has two binding nitrogen atoms and four oxygen atoms from carboxyl groups and it forms complexes with almost all metal ions. EDTA as a tetraprotonic acid, H4Y disociates through four steps, yielding the ions HsY-, H2Y2-, HY3- and Y4-. Which of the EDTA forms will be encountered in a solution, depends on the pH. Due to the poor solubility of EDTA in pure water, as well as in most organic solvents, the disodium salt of EDTA Na2H2Y-2H2O, under the commercial name complexon III, is utilized for analytical determinations. In water, EDTA forms soluble, stabile chelate complexes with all cations, at the molar ratio 1:1, regardless of the charge of the metal ion. In contrast to other equilibria, which are mainly defined by Le Chatellier's principle, equilibria related to metal-EDTA complex formation are also dependent on the influence of the secondary equilibria of EDTA complex formation. Complexing reactions, which are equilibrium reactions, are simultaneously influenced by the following factors: solution pH and the presence of complexing agents which may also form a stabile complex with metal ions. The secondary reaction influence may be viewed and monitored through conditional stability constants. In the first part of the paper, the reaction of the formation of the Cu2+-ion complex with EDTA is analyzed beginning from the main reaction through various influences of secondary reactions on the complex Cu2+-EDTA: pH effect, complexation effect and hydrolysis effect. The equations are given for conditional stability constants, which include equilibrium reactions under actual conditions.

  7. Extraction of rare earth elements from a contaminated cropland soil using nitric acid, citric acid, and EDTA.

    Science.gov (United States)

    Tang, Hailong; Shuai, Weitao; Wang, Xiaojing; Liu, Yangsheng

    2017-08-01

    Rare earth elements (REEs) contamination to the surrounding soil has increased the concerns of health risk to the local residents. Soil washing was first attempted in our study to remediate REEs-contaminated cropland soil using nitric acid, citric acid, and ethylene diamine tetraacetic acid (EDTA) for soil decontamination and possible recovery of REEs. The extraction time, washing agent concentration, and pH value of the washing solution were optimized. The sequential extraction analysis proposed by Tessier was adopted to study the speciation changes of the REEs before and after soil washing. The extract containing citric acid was dried to obtain solid for the X-ray fluorescence (XRF) analysis. The results revealed that the optimal extraction time was 72 h, and the REEs extraction efficiency increased as the agent concentration increased from 0.01 to 0.1 mol/L. EDTA was efficient to extract REEs over a wide range of pH values, while citric acid was around pH 6.0. Under optimized conditions, the average extraction efficiencies of the major REEs in the contaminated soil were 70.96%, 64.38%, and 62.12% by EDTA, nitric acid, and citric acid, respectively. The sequential extraction analyses revealed that most soil-bounded REEs were mobilized or extracted except for those in the residual fraction. Under a comprehensive consideration of the extraction efficiency and the environmental impact, citric acid was recommended as the most suitable agent for extraction of the REEs from the contaminated cropland soils. The XRF analysis revealed that Mn, Al, Si, Pb, Fe, and REEs were the major elements in the extract indicating a possibile recovery of the REEs.

  8. Reactivity of OH· and e-aq from electron beam irradiation of aqueous solutions of EDTA and aminopolycarboxylic acids

    International Nuclear Information System (INIS)

    Vel Leitner, N.K.; Guilbault, I.; Legube, B.

    2003-01-01

    Electron beam irradiation of aqueous solutions of EDTA, EDDA NN', NTA, IDA and Cu-EDTA was performed in the presence of scavengers for the hydroxyl radicals (methanol) or for the solvated electrons (hydrogen peroxide). Experiments showed that for each molecule, the G-value decreases as the radiation dose increases from 1 to 25 kGy, and for EDTA, when the initial concentration decreases from 10 to 0.1 mmol l -1 . At pH 8 and for 5 kGy, the G-values of NTA, IDA, EDTA and EDDA NN' removal ascribed to OH · radicals are, respectively, 0.06, 0.06, 0.15 and 0.20 μmol J -1 , whereas for the solvated electrons the G-values were, respectively, 0.01, 0.01, 0.06 and 0.04 μmol J -1 . The rate constants of hydroxyl radicals and solvated electrons were determined by comparison with one competitor. For each active species (hydroxyl radical or solvated electron), the reactivity is connected to the number of nitrogen atoms and acetate groups. The rate constants of OH · radicals are above 10 10 and 8.6x10 9 l mol -1 s -1 for EDDA NN' and EDTA, respectively, 2.1x10 9 l mol -1 s -1 for IDA and 6.1x10 8 l mol -1 s -1 for NTA. The reactivity of solvated electrons is smaller and the rate constants are in the range 1.9x10 6 -3.7x10 6 l mol -1 s -1 for NTA, IDA and EDDA NN' and equal 1.4x10 7 l mol -1 s -1 for EDTA. The reactivity of the complex Cu-EDTA towards OH · does not differ to a large extent from EDTA whereas with e - aq the reactivity of Cu-EDTA is better than EDTA since k e - /Cu-EDTA reaches 2.2x10 9 l mol -1 s -1 . It follows that when both active entities (OH · and e - aq ) are involved in the electron beam irradiation process, the removal of free aminopolycarboxylic acids is mainly due to OH · radicals. However, the complex Cu-EDTA is concerned by both e - aq and OH · radicals

  9. Response of the periapical tissue of dogs' teeth to the action of citric acid and EDTA

    Directory of Open Access Journals (Sweden)

    Cristina Berthold Sperandio

    2008-02-01

    Full Text Available The purpose of this study was to analyze the inflammatory response of dog's periapical tissues to 17% trisodium EDTA salt (pH 8.0 and 1% citric acid (pH 2.0. Saline was used as a control. Six adult dogs were used as the biological model of the study. The experimental units comprised 56 roots of mandibular molars (first and second and premolars (first, second and third. After coronal opening, pulpectomy and root canal instrumentation were performed using the above-mentioned irrigating solutions. After 24 and 48 hours, the animals were euthanized and the teeth and their supporting tissues were removed and histologically processed. The sections were stained with hematoxylin and eosin and analyzed histopathologically with a light microscope at x100 magnification. The histological analysis focused on the occurrence of acute inflammatory response. The presence of swelling, vasodilatation and inflammatory cells were evaluated and the degree of inflammation was determined for each case. Data were analyzed by Fisher's exact test using the SPSS software with a confidence interval of 95% (p<0.05. 17% EDTA and 1% citric acid caused inflammatory responses in dog's periapical tissues with no significant differences to each other or to saline (control at either the 24-hour (p=0.482 or 48-hour (p=0.377 periods. It may be concluded that the inflammatory response was of mild intensity for the tested substances.

  10. EDTA and HCl leaching of calcareous and acidic soils polluted with potentially toxic metals: remediation efficiency and soil impact.

    Science.gov (United States)

    Udovic, Metka; Lestan, Domen

    2012-07-01

    The environmental risk of potentially toxic metals (PTMs) in soil can be diminished by their removal. Among the available remediation techniques, soil leaching with various solutions is one of the most effective but data about the impact on soil chemical and biological properties are still scarce. We studied the effect of two common leaching agents, hydrochloric acid (HCl) and a chelating agent (EDTA) on Pb, Zn, Cd removal and accessibility and on physico-chemical and biological properties in one calcareous, pH neutral soil and one non-calcareous acidic soil. EDTA was a more efficient leachant compared to HCl: up to 133-times lower chelant concentration was needed for the same percentage (35%) of Pb removal. EDTA and HCl concentrations with similar PTM removal efficiency decreased PTM accessibility in both soils but had different impacts on soil properties. As expected, HCl significantly dissolved carbonates from calcareous soil, while EDTA leaching increased the pH of the acidic soil. Enzyme activity assays showed that leaching with HCl had a distinctly negative impact on soil microbial and enzyme activity, while leaching with EDTA had less impact. Our results emphasize the importance of considering the ecological impact of remediation processes on soil in addition to the capacity for PTM removal. Copyright © 2012 Elsevier Ltd. All rights reserved.

  11. Antimicrobial activity optimization of nisin, ascorbic acid and ethylenediamine tetraacetic acid disodium salt (EDTA against Salmonella Enteritidis ATCC 13076 using response surface methodology

    Directory of Open Access Journals (Sweden)

    Nongpanga Sangcharoen

    2017-10-01

    Full Text Available Nisin is a commercial bacteriocin produced by Lactococcus lactis subsp. lactis and widely used as a natural preservative in the food industry. However, while nisin alone cannot inhibit the growth of Gram-negative bacteria, it can in combination with a chelating agent or organic acid. This study combined nisin with some chelating agents, weak organic acids and their salts to inhibit Salmonella Enteritidis ATCC 13076. The combinations of nisin (2000 parts per million; ppm and ascorbic acid (2000 ppm or ethylenediamine tetraacetic acid disodium salt (EDTA; 7400 ppm showed significant inhibitory effects on the target strain. Due to regulatory limits, the second part of the study reduced the concentrations of nisin, ascorbic acid and EDTA to 500 ppm, 2000 ppm and 250 ppm, respectively. The mixture of nisin, ascorbic acid and EDTA showed the highest inhibitory effect with a reduction number of 3.41 log colony forming units (p < 0.05. To minimize the growth of S. Enteritidis ATCC 13076, central composite design and response surface methodology were applied to investigate the combined effect of nisin (0–500 ppm, ascorbic acid (0–2000 ppm, and EDTA (0–250 ppm on the target strain growth. Among the three factors, nisin had a higher antimicrobial effect than ascorbic acid or EDTA, while an increase in nisin resulted in a decrease in S. Enteritidis ATCC 13076 growth. The optimum concentration was 500 ppm nisin with 1515 ppm ascorbic acid and 250 ppm EDTA. Under these conditions, the growth of S. Enteritidis ATCC 13076 predicted by the model was 24.99%. Keywords: Antimicrobial activity, Ascorbic acid, Ethylenediamine tetraacetic acid disodium salt (EDTA, Nisin, Salmonella enteritidis

  12. EDTA assisted synthesis of hydroxyapatite nanoparticles for electrochemical sensing of uric acid.

    Science.gov (United States)

    Kanchana, P; Sekar, C

    2014-09-01

    Hydroxyapatite nanoparticles have been synthesized using EDTA as organic modifier by a simple microwave irradiation method and its application for the selective determination of uric acid (UA) has been demonstrated. Electrochemical behavior of uric acid at HA nanoparticle modified glassy carbon electrode (E-HA/GCE) has been investigated by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), linear sweep voltammetry (LSV) and amperometry. The E-HA modified electrode exhibits efficient electrochemical activity towards uric acid sensing without requiring enzyme or electron mediator. Amperometry studies revealed that the fabricated electrode has excellent sensitivity for uric acid with the lowest detection limit of 142 nM over a wide concentration range from 1 × 10(-7) to 3 × 10(-5)M. Moreover, the studied E-HA modified GC electrode exhibits a good reproducibility and long-term stability and an admirable selectivity towards the determination of UA even in the presence of potential interferents. The analytical performance of this sensor was evaluated for the detection of uric acid in human urine and blood serum samples. Copyright © 2014. Published by Elsevier B.V.

  13. The influence of using anticoagulants (EDTA and citrate acid 3.8% toward the quantity of Platelet Rich Plasma (PRP

    Directory of Open Access Journals (Sweden)

    Lilies Anggarwati Astuti

    2016-06-01

    Full Text Available Platelet Rich Plasma (PRP is a blood concentrate that has a thrombocytes concentration several time higher than normal concentration of thrombocytes in normal human blood. PRP is a promising alternative to surgery with a safe and natural healing. The standard protocol for PRP preparation must be determined to get the right quantity and quality of the matrix of fibrin, leukocytes, platelets and growth factors. It could not be separated from the number of PRP produced. The use of PRP in the success of periodontal treatment would not be separated from methods to obtain it. To detect the influence of using anticoagulants (EDTA and citrate acid 3.8% toward the quantity of PRP. There are 41 subjects studied by taking 21 ml of venous blood in each of the seven tubes. Centrifugation performed twice with different speed, duration, use of anticoagulants then analyzed. This quantity between the two groups differed significantly between the PRP in EDTA group is higher 322.2 ml rather than citrate acid 3.8% group, then control group is higher 329.5 ml rather than citrate acid 3.8% group, while there is no difference between EDTA and control group. There is effect of the use of anticoagulants EDTA compared with citrate acid 3.8% in the quantity of PRP, and there was no effect using citrate acid 3.8% as anticoagulants in quantity of PRP.

  14. Isolation and growth characteristics of an EDTA-degrading member of the alpha-subclass of Proteobacteria.

    Science.gov (United States)

    Weilenmann, Hans-Ueli; Engeli, Barbara; Bucheli-Witschel, Margarete; Egli, Thomas

    2004-10-01

    A Gram-negative, ethylenediaminetetraacetic acid (EDTA)-degrading bacterium (deposited at the German Culture Collection as strain DSM 9103) utilising EDTA as the only source of carbon, energy and nitrogen was isolated from a mixed EDTA-degrading population that was originally enriched in a column system from a mixture of activated sludge and soil. Chemotaxonomic analysis of quinones, polar lipids and fatty acids allowed allocation of the isolate to the alpha-subclass of Proteobacteria. 16S rDNA sequencing and phylogenetic analysis revealed highest similarity to the Mesorhizobium genus followed by the Aminobacter genus. However, the EDTA-degrading strain apparently forms a new branch within the Phyllobacteriaceae/Mesorhizobia family. Growth of the strain was rather slow not only on EDTA (micro(max) = 0.05 h(-1)) but also on other substrates. Classical substrate utilisation testing in batch culture suggested a quite restricted carbon source spectrum with only lactate, glutamate, and complexing agents chemically related to EDTA (nitrilotriacetate, iminodiacetate and ethylenediaminedisuccinate) supporting growth. However, when EDTA-limited continuous cultures of strain DSM 9103 were pulsed with fumarate, succinate, glucose or acetate, these substrates were assimilated immediately. Apparently, the strain can use a broader spectrum than indicated by traditional substrate testing techniques. The EDTA species CaEDTA and MgEDTA served as growth substrates of the strain because in the mineral medium employed EDTA was predicted to be mainly present in the form of these two complexes. The bacterium was not able to degrade Fe3+-complexed EDTA.

  15. Highly concentrated EDTA gel improves cleaning efficiency of root canal preparation in vitro.

    Science.gov (United States)

    Putzer, P; Hoy, L; Günay, H

    2008-12-01

    Debris and smear layer, as a product of mechanical root canal instrumentation, reduce the effectiveness of pharmacological substances to prevent post-treatment diseases and impair direct contact of filling materials with a clean dentinal surface. The aim of this in vitro study was to investigate the presence and localization of debris and smear layer via scanning electron microscope analysis after standardized root canal preparation with different chelating agents. Dentin surfaces received treatment with: (1) 15% ethylenediaminetetraacetic acid (EDTA), (2) 18.6% EDTA (3) and 24% EDTA or without any demineralizing chemicals as control. Forty vertically split human premolars were sputtered and divided into coronal, middle, and apical sections, followed by a randomized, blinded score evaluation using five scores. Pairwise comparisons of all treatment groups against a control group have been performed by Mann-Whitney U test and the Kruskal-Wallis test. Debris grades showed no significant difference between the three regions of the root canals, except for 18.6% EDTA in the central third. Smear layer and smear plug removal was concentration-dependent. Removal of the smear layer in the three areas showed that there was a statistically significant difference between all parts when using 18.6% and 24% EDTA concentrations compared with the control. The best smear layer removal in the apical region was observed using a 24% EDTA gel as chelating agent and lubricant. The usage of EDTA gel >/=18.6% presented a better cleaning regime when compared to the control group.

  16. "Off-the-Shelf" K2-EDTA for Calcific Band Keratopathy.

    Science.gov (United States)

    Lee, Marco E; Ouano, Dean P; Shapiro, Brett; Fong, Andrew; Coroneo, Minas T

    2018-07-01

    To explore the effectiveness of "off-the-shelf" dipotassium-ethylenediaminetetraacetic acid (K2-EDTA) as an alternative to sodium EDTA as chelation therapy in removal of calcific band keratopathy (CBK). This study was a retrospective case series involving 4 patients with CBK who underwent superficial keratectomy and subsequent chelation therapy with K2-EDTA in a single center (Coastal Eye Clinic) by the same surgeon. Visual acuity and symptomatic relief were the main outcomes measured in our study. All 4 participants in this study were female with an average age of 80.3 years. Three of the patients with reasonable baseline visual acuity experienced improved visual acuity at 1 month. The other patient with multiple ocular comorbidities and severely reduced visual potential reported symptomatic pain relief at 1-month follow-up after the intervention. K2-EDTA seems to be an effective alternative to disodium EDTA in its ability to clear calcific plaques and restore visual function. Because of the logistical difficulties associated with acquiring disodium EDTA, and the relative abundance of K2-EDTA in health-care facilities, we believe that our findings warrant further investigation into its use as a more accessible and cost-effective chelating agent in CBK.

  17. EDTA-assisted synthesis of rose-like ZnO architectures

    Energy Technology Data Exchange (ETDEWEB)

    Li, Zhen [Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai 201800 (China); Shanghai Applied Radiation Institute, Shanghai University, Shanghai 201800 (China); Fang, Yaoguo [Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai 201800 (China); Peng, Liwei; Wu, Minghong [Shanghai Applied Radiation Institute, Shanghai University, Shanghai 201800 (China); Pan, Dengyu

    2010-10-15

    Rose-like ZnO nanostructures were prepared by a low-temperature solution route with assistance of ethylenediaminetetraacetic acid disodium (EDTA-2Na). The morphology of ZnO nanostructures was found to change from nanowire arrays to rose- and tower-like architectures with increasing the molar ratio of EDTA-2Na/Zn{sup 2+}. Also, the shape evolution of ZnO nanostructures with time was observed from flat nanosheets to wrinkled nanosheets and to rose-like nanostructures. EDTA-2Na as a strong complexing agent was found to play a key role in the shape evolution. Photoluminescence spectra show that the rose-like ZnO architectures have more defects than the nanowire arrays. (copyright 2010 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  18. Effects of calcium disodium EDTA and meso-2,3-dimercaptosuccinic acid on tissue concentrations of lead for use in treatment of calves with experimentally induced lead toxicosis.

    Science.gov (United States)

    Meldrum, James B; Ko, Kam W

    2003-06-01

    To compare the efficacy of calcium disodium EDTA (CaNa2EDTA) and meso-2,3-dimercaptosuccinic acid (DMSA) in reducing concentrations of lead in selected tissues for use in treatment of calves with experimentally induced lead toxicosis. 19 sexually intact male Holstein calves that weighed 35 to 60 kg. Calves were randomly assigned to 1 of 5 treatment groups: group 1, control calves; group 2, lead only; group 3, lead and EDTA; group 4, lead and DMSA; and group 5, lead, EDTA, and DMSA. Calves in groups 2 to 5 were dosed daily with lead (5 mg/kg, PO) for 10 days. Doses of EDTA (100 mg/kg) and DMSA (25 mg/kg) were administered IV once daily for 4 consecutive days beginning on day 11. Effects of the chelators on lead concentrations in the liver, kidneys, testes, muscles, bones, and brain were compared among the various groups. Compared with the effects of EDTA, DMSA greatly reduced lead concentrations in renal and hepatic tissues. We did not detect significant differences for the effects of EDTA or DMSA on lead concentrations in the testes; there was an adverse interaction of EDTA with DMSA that caused an increase in lead concentrations in the testes. DMSA is much more effective than EDTA in removing lead from renal and hepatic tissues in calves. Use of DMSA in calves with lead intoxication appears to be a viable treatment option. Combining DMSA and EDTA as a treatment modality in calves did not offer any advantages.

  19. Biodegradation of PuEDTA and Impacts on Pu Mobility

    International Nuclear Information System (INIS)

    Xun, Luying; Bolton, Jr. Harvey

    2001-01-01

    Ethylenediaminetetraacetate (EDTA) and nitrilotriacetate (NTA) are synthetic chelating agents, which can form strong water-soluble complexes with radionuclides and metals and has been used to decontaminate and process nuclear materials. Synthetic chelating agents were co-disposed with radionuclides (e.g., 60Co, Pu) and heavy metals enhancing their transport in the subsurface. An understanding of EDTA biodegradation is essential to help mitigate enhanced radionuclide transport by EDTA. The objective of this research is to develop fundamental data on factors that govern the biodegradation of radionuclide-EDTA. These factors include the dominant EDTA aqueous species, the biodegradation of various metal-EDTA complexes, the uptake of various metal-EDTA complexes into the cell, the distribution and mobility of the radionuclide during and after EDTA biodegradation, and the enzymology and genetics of EDTA biodegradation

  20. Influence of EDTA on lead transportation and accumulation by Sedum alfredii Hance

    Energy Technology Data Exchange (ETDEWEB)

    Liu Dan [Zhejiang Univ., Hangzhou (China). Key Lab. of Environmental Remediation and Ecosystem Health, Ministry of Education; Zhejiang Forestry College, Lin' an (China). School of Tourism and Health; Li Ting-Qiang; Yang Xiao-E; Islam, E.; Jin Xiao-Fen; Mahmood, Q. [Zhejiang Univ., Hangzhou (China). Key Lab. of Environmental Remediation and Ecosystem Health, Ministry of Education

    2007-09-15

    Hydroponics and pot experiments were conducted to study the effects of ethylenediaminetetraacetic acid (EDTA) on Pb transportation and accumulation by two contrasting ecotypes of Sedum alfredii Hance. In hydroponics experiments, the accumulating ecotype (AE) showed more ability to tolerate Pb toxicitycompared with the non-accumulating ecotype (NAE). When treated with equimolar mixtures of EDTA and Pb, maximum Pb accumulation occurred without any phytotoxicity symptoms. Pot experiments with Pb contents of 400 mg kg{sup -1} showed that 5 mm EDTA is the optimum dose for the phytoextraction of soils contaminated with relatively low Pb levels; in contrast, increasing EDTA addition resulted in increased Pb accumulation in the shoots of AE in soils with high Pb content (1200 mg kg{sup -1}). The post-harvest effects of EDTA on available Pb were strong compared with those without addition of EDTA (CK). Within the initial 7 days almost no differences of water-soluble Pb were noted in soils contaminated with both levels of Pb but after 2 weeks, water-soluble Pb started to decrease significantlycompared with before. Considering the toxicity and biodegradability of synthetic chelators, it can be concluded that the chelate-assisted technique is more suitable for soils contaminated with low Pb levels and to avoid environment risks; a suitable dose of chelators must be considered before application. (orig.)

  1. Electrochemical Oxidation of EDTA in Nuclear Wastewater Using Platinum Supported on Activated Carbon Fibers

    Science.gov (United States)

    Zhao, Bo; Zhu, Wenkun; Mu, Tao; Hu, Zuowen; Duan, Tao

    2017-01-01

    A novel Pt/ACF (Pt supported on activated carbon fibers) electrode was successfully prepared with impregnation and electrodeposition method. Characterization of the electrodes indicated that the Pt/ACF electrode had a larger effective area and more active sites. Electrochemical degradation of ethylenediaminetetra-acetic acid (EDTA) in aqueous solution with Pt/ACF electrodes was investigated. The results showed that the 3% Pt/ACF electrode had a better effect on EDTA removal. The operational parameters influencing the electrochemical degradation of EDTA with 3% Pt/ACF electrode were optimized and the optimal removal of EDTA and chemical oxygen demand (COD) were 94% and 60% after 100 min on condition of the electrolyte concentration, initial concentration of EDTA, current density and initial value of pH were 0.1 mol/L, 300 mg/L, 40 mA/cm2 and 5.0, respectively. The degradation intermediates of EDTA in electrochemical oxidation with 3% Pt/ACF electrode were identified by gas chromatography-mass spectrum (GC-MS). PMID:28754016

  2. Electrochemical Oxidation of EDTA in Nuclear Wastewater Using Platinum Supported on Activated Carbon Fibers.

    Science.gov (United States)

    Zhao, Bo; Zhu, Wenkun; Mu, Tao; Hu, Zuowen; Duan, Tao

    2017-07-21

    A novel Pt/ACF (Pt supported on activated carbon fibers) electrode was successfully prepared with impregnation and electrodeposition method. Characterization of the electrodes indicated that the Pt/ACF electrode had a larger effective area and more active sites. Electrochemical degradation of ethylenediaminetetra-acetic acid (EDTA) in aqueous solution with Pt/ACF electrodes was investigated. The results showed that the 3% Pt/ACF electrode had a better effect on EDTA removal. The operational parameters influencing the electrochemical degradation of EDTA with 3% Pt/ACF electrode were optimized and the optimal removal of EDTA and chemical oxygen demand (COD) were 94% and 60% after 100 min on condition of the electrolyte concentration, initial concentration of EDTA, current density and initial value of pH were 0.1 mol/L, 300 mg/L, 40 mA/cm² and 5.0, respectively. The degradation intermediates of EDTA in electrochemical oxidation with 3% Pt/ACF electrode were identified by gas chromatography-mass spectrum (GC-MS).

  3. Dissolution of nickel ferrite and rare earths containing magnetites in citric acid - EDTA - gallic acid (CEG) formulation

    International Nuclear Information System (INIS)

    Kumbhar, A.G.; Kishore, K.; Venkateswaran, G.; Balaji, V.

    2002-01-01

    It has been shown by us earlier that gallic acid can be used as a reductant in dilute chemical decontaminant formulations containing EDTA as chelant. The results on the dissolution of magnetite in such a formulation were quite promising. Moreover, the superior radiation stability of gallic acid vis-a-vis other reductants such as ascorbic acid or oxalic acid is another plus point for this formulation. Besides having an inherent stability against radiation degradation, it is able to protect even EDTA against radiation induced decomposition to a great extent unlike the case of ascorbic acid. In an extension of that work, dissolution experiments have now been carried out on nickel ferrite and magnetites containing rare earths like La, Ce and Zr This is to simulate the presence of fission product oxides in magnetite resulting from a possible phase of operation with leaky fuel. The rate constants have been determined using the inverse cubic rate law. In the case of nickel ferrite, although there is an initial induction period, the rate constants for the dissolution were determined to be at 1.6 x 10 -2 and 3.6 x 10 -3 min -1 at 353 K and 333 K respectively. Presence of Ce in particular either alone or in combination with Zr/La at a level of 1 at.% equivalent each in magnetite is seen to increase the surface area of the oxide. The rate constants for the dissolution at 353 K in a 11:44:4 mM CEG formulation taken with magnetite and rare earth containing magnetites equivalent to yielding 22 mM Fe upon complete dissolution are as follows: 5.09 x 10 -2 min -1 (magnetite), 7.06 x 10 -2 min -1 (Ce 2 O 3 containing magnetite), 6.33 x 10 -2 min -1 (Ce 2 O 3 , ZrO 2 containing magnetite), 1 x 10 -1 min -1 (Ce 2 O 3 , ZrO 2 , La 2 O 3 containing magnetite). The presence of Ce, Zr and La at 1 at.% level each has not resulted in any turbidity in solution at the end of magnetite dissolution suggesting chemical dissolution of these rare earth oxides in the formulation. Simple magnetite

  4. Dissolution of nickel ferrite and rare earths containing magnetites in citric acid - EDTA - gallic acid (CEG) formulation

    Energy Technology Data Exchange (ETDEWEB)

    Kumbhar, A.G.; Kishore, K.; Venkateswaran, G.; Balaji, V. [Applied Chemistry Div., Bhabha Atomic Research Centre, Mumbai (India)

    2002-07-01

    It has been shown by us earlier that gallic acid can be used as a reductant in dilute chemical decontaminant formulations containing EDTA as chelant. The results on the dissolution of magnetite in such a formulation were quite promising. Moreover, the superior radiation stability of gallic acid vis-a-vis other reductants such as ascorbic acid or oxalic acid is another plus point for this formulation. Besides having an inherent stability against radiation degradation, it is able to protect even EDTA against radiation induced decomposition to a great extent unlike the case of ascorbic acid. In an extension of that work, dissolution experiments have now been carried out on nickel ferrite and magnetites containing rare earths like La, Ce and Zr This is to simulate the presence of fission product oxides in magnetite resulting from a possible phase of operation with leaky fuel. The rate constants have been determined using the inverse cubic rate law. In the case of nickel ferrite, although there is an initial induction period, the rate constants for the dissolution were determined to be at 1.6 x 10{sup -2} and 3.6 x 10{sup -3} min{sup -1} at 353 K and 333 K respectively. Presence of Ce in particular either alone or in combination with Zr/La at a level of 1 at.% equivalent each in magnetite is seen to increase the surface area of the oxide. The rate constants for the dissolution at 353 K in a 11:44:4 mM CEG formulation taken with magnetite and rare earth containing magnetites equivalent to yielding 22 mM Fe upon complete dissolution are as follows: 5.09 x 10{sup -2} min{sup -1} (magnetite), 7.06 x 10{sup -2} min{sup -1} (Ce{sub 2}O{sub 3} containing magnetite), 6.33 x 10{sup -2} min{sup -1} (Ce{sub 2}O{sub 3}, ZrO{sub 2} containing magnetite), 1 x 10{sup -1} min{sup -1} (Ce{sub 2}O{sub 3}, ZrO{sub 2}, La{sub 2}O{sub 3} containing magnetite). The presence of Ce, Zr and La at 1 at.% level each has not resulted in any turbidity in solution at the end of magnetite dissolution

  5. Interactions between the Tetrasodium Salts of EDTA and 1-Hydroxyethane 1,1-Diphosphonic Acid with Sodium Hypochlorite Irrigants.

    Science.gov (United States)

    Biel, Philippe; Mohn, Dirk; Attin, Thomas; Zehnder, Matthias

    2017-04-01

    A clinically useful all-in-one endodontic irrigant with combined proteolytic and decalcifying properties is still elusive. In this study, the chemical effects of dissolving the tetrasodium salts of 1-hydroxyethane 1,1-diphosphonic acid (Na 4 HEDP) or Na 4 EDTA directly in sodium hypochlorite (NaOCl) irrigants in polypropylene syringes were assessed during the course of 1 hour. The solubility of the salts in water was determined. Their compatibility with 1% and 5% NaOCl was measured by iodometric titration and in a calcium complexation experiment by using a Ca 2+ -selective electrode. The salts dissolved within 1 minute. The dissolution maximum of Na 4 HEDP in water (wt/total wt) was 44.6% ± 1.6%. The corresponding dissolution maximum of Na 4 EDTA was 38.2% ± 0.8%. Na 4 HEDP at 18% in 5% NaOCl caused a mere loss of 16% of the initially available chlorine during 1 hour. In contrast, a corresponding mixture between NaOCl and the Na 4 EDTA salt caused 95% reduction in available chlorine after 1 minute. Mixtures of 3% Na 4 EDTA with 1% NaOCl were more stable, but only for 30 minutes. Na 4 HEDP lost 24% of its calcium complexation capacity after 60 minutes. The corresponding loss for Na 4 EDTA was 34%. The compatibility and solubility of particulate Na 4 HEDP with/in NaOCl solutions are such that these components can be mixed and used for up to 1 hour. In contrast, short-term compatibility of the Na 4 EDTA salt with NaOCl solutions was considerably lower, decreasing at higher concentrations of either compound. Especially for Na 4 HEDP but also for Na 4 EDTA, the NaOCl had little effect on calcium complexation. Copyright © 2017 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  6. Redox-initiated poly(methyl methcrylate) emulsion polymerizations stabilized with block copolymers based on poly(ethylene oxide), e-caprolactone and linoleic acid

    NARCIS (Netherlands)

    Tan, B.H.; Nabuurs, Tijs; Feijen, Jan; Grijpma, Dirk W.

    2009-01-01

    A redox initiating system, consisting of t-butyl hydroperoxide (tBHPO), isoascorbic acid (iAA), and ethylenediaminetetraacetic acid ferric-sodium salt (FeEDTA) was employed in emulsion polymerizations of methyl methacrylate (MMA) at high solids contents of 30 wt % in water. The system was stabilized

  7. Redox-Initiated Poly(methyl methacrylate) Emulsion Polymerizations Stabilized with Block Copolymers Based on Methoxy-Poly(ethylene glycol), epsilon-Caprolactone, and Linoleic Acid

    NARCIS (Netherlands)

    Tan, Boonhua; Nabuurs, Tijs; Feijen, Jan; Grijpma, Dirk W.

    2009-01-01

    A redox initiating system, consisting of t-butyl hydroperoxide (tBHPO), isoascorbic acid (iAA), and ethylenediaminetetraacetic acid ferric-sodium salt (FeEDTA) was employed in emulsion polymerizations of methyl methacrylate (MMA) at high solids contents of 30 wt % in water. The system was stabilized

  8. The effect of EDTA and citric acid on phytoremediation of Cd, Cr, and Ni from soil using Helianthus annuus

    International Nuclear Information System (INIS)

    Turgut, Cafer; Katie Pepe, M.; Cutright, Teresa J.

    2004-01-01

    The possibility to clean heavy metal contaminated soils with hyperaccumulator plants has shown great potential. One of the most recently studied species used in phytoremediation applications are sunflowers. In this study, two cultivars of Helianthus annuus were used in conjunction with ethylene diamine tetracetic acid (EDTA) and citric acid (CA) as chelators. Two different concentrations of the chelators were studied for enhancing the uptake and translocation of Cd, Cr, and Ni from a silty-clay loam soil. When 1.0 g/kg CA was used, the highest total metal uptake was only 0.65 mg. Increasing the CA concentration posed a severe phytotoxicity to both cultivars as evidenced by stunted growth and diminished uptake rates. Decreasing the CA concentration to 0.1 and 0.3 g/kg yielded results that were not statistically different from the control. EDTA at a concentration of 0.1 g/kg yielded the best results for both cultivars achieving a total metal uptake of ∼0.73 mg compared to ∼0.40 mg when EDTA was present at 0.3 g/kg

  9. The effect of EDTA and citric acid on phytoremediation of Cd, Cr, and Ni from soil using Helianthus annuus.

    Science.gov (United States)

    Turgut, Cafer; Katie Pepe, M; Cutright, Teresa J

    2004-09-01

    The possibility to clean heavy metal contaminated soils with hyperaccumulator plants has shown great potential. One of the most recently studied species used in phytoremediation applications are sunflowers. In this study, two cultivars of Helianthus annuus were used in conjunction with ethylene diamine tetracetic acid (EDTA) and citric acid (CA) as chelators. Two different concentrations of the chelators were studied for enhancing the uptake and translocation of Cd, Cr, and Ni from a silty-clay loam soil. When 1.0 g/kg CA was used, the highest total metal uptake was only 0.65 mg. Increasing the CA concentration posed a severe phytotoxicity to both cultivars as evidenced by stunted growth and diminished uptake rates. Decreasing the CA concentration to 0.1 and 0.3 g/kg yielded results that were not statistically different from the control. EDTA at a concentration of 0.1 g/kg yielded the best results for both cultivars achieving a total metal uptake of approximately 0.73 mg compared to approximately 0.40 mg when EDTA was present at 0.3 g/kg.

  10. The effect of EDTA and citric acid on phytoremediation of Cd, Cr, and Ni from soil using Helianthus annuus

    Energy Technology Data Exchange (ETDEWEB)

    Turgut, Cafer; Katie Pepe, M.; Cutright, Teresa J

    2004-09-01

    The possibility to clean heavy metal contaminated soils with hyperaccumulator plants has shown great potential. One of the most recently studied species used in phytoremediation applications are sunflowers. In this study, two cultivars of Helianthus annuus were used in conjunction with ethylene diamine tetracetic acid (EDTA) and citric acid (CA) as chelators. Two different concentrations of the chelators were studied for enhancing the uptake and translocation of Cd, Cr, and Ni from a silty-clay loam soil. When 1.0 g/kg CA was used, the highest total metal uptake was only 0.65 mg. Increasing the CA concentration posed a severe phytotoxicity to both cultivars as evidenced by stunted growth and diminished uptake rates. Decreasing the CA concentration to 0.1 and 0.3 g/kg yielded results that were not statistically different from the control. EDTA at a concentration of 0.1 g/kg yielded the best results for both cultivars achieving a total metal uptake of {approx}0.73 mg compared to {approx}0.40 mg when EDTA was present at 0.3 g/kg.

  11. The Effect of EDTA and Citric acid on Soil Enzymes Activity, Substrate Induced Respiration and Pb Availability in a Contaminated Soil

    Directory of Open Access Journals (Sweden)

    seyed sajjad hosseini

    2017-03-01

    Full Text Available Introduction: Application of EDTA may increase the heavy metal availability and phytoextraction efficiency in contaminated soils. In spite of that, it might also have some adverse effects on soil biological properties. Metals as freeions are considered to be severely toxic, whereas the complexed form of these metalswith organic compounds or Fe/Mn oxides may be less available to soil microbes. However, apart from this fact, some of these compounds like EDTA and EDTA-metal complexes have low bio- chemo- and photo-degradablity and high solubility in their own characteristics andable to cause toxicity in soil environment. So more attentions have been paid to use of low molecular weight organic acids (LMWOAs such as Citric acid because of having less unfavorable effects to the environment. Citric acid increases heavy metals solubility in soils and it also improves soil microbial activity indirectly. Soil enzymes activity is a good indicator of soil quality, and it is more suitable for monitoring the soil quality compared to physical or chemical indicators. The aims of this research were to evaluate the changes of dehydrogenase, urease and alkaline phosphomonoesterase activities, substrate-induced respiration (SIR and Pb availability after EDTA and citric acid addition into a contaminated soil with PbCl2. Materials and Methods: An experiment was conducted in a completely randomized design with factorial arrangement and three replications in greenhouse condition. The soil samples collected from surface horizon (0-20 cm of the Typic haplocalsids, located in Mashhad, Iran. Soil samples were artificially contaminated with PbCl2 (500 mg Pb per kg of soil and incubated for one months in 70 % of water holding capacity at room temperature. The experimental treatments included control, 3 and 5 mmol EDTA (EDTA3 and EDTA5 and Citric acid (CA3 and CA5 per kg of soil. Soil enzymes activity, substrate-induced respiration and Pb availability of soil samples were

  12. Oxidative degradation stability and hydrogen sulfide removal performance of dual-ligand iron chelate of Fe-EDTA/CA.

    Science.gov (United States)

    Miao, Xinmei; Ma, Yiwen; Chen, Zezhi; Gong, Huijuan

    2017-09-05

    Catalytic oxidation desulfurization using chelated iron catalyst is an effective method to remove H 2 S from various gas streams including biogas. However, the ligand of ethylenediaminetetraacetic acid (EDTA), which is usually adopted to prepare chelated iron catalyst, is liable to be oxidative degraded, and leads to the loss of desulfurization performance. In order to improve the degradation stability of the iron chelate, a series of iron chelates composed of two ligands including citric acid (CA) and EDTA were prepared and the oxidative degradation stability as well as desulfurization performance of these chelated iron catalysts were studied. Results show that the iron chelate of Fe-CA is more stable than Fe-EDTA, while for the desulfurization performance, the situation is converse. For the dual-ligand iron chelates of Fe-EDTA/CA, with the increase of mol ratio of CA to EDTA in the iron chelate solution, the oxidative degradation stability increased while the desulfurization performance decreased. The results of this work showed that Fe-EDTA/CA with a mol ratio of CA:EDTA = 1:1 presents a relative high oxidative degradation stability and an acceptable desulfurization performance with over 90% of H 2 S removal efficiency.

  13. Reducing the potential for migration of radioactive waste: Aqueous thermal degradation of the chelating agent disodium EDTA

    International Nuclear Information System (INIS)

    Boles, J.S.; Ritchie, K.; Crerar, D.A.

    1987-01-01

    Ethylenediaminetetraacetic acid (EDTA), a common component of cleaning solutions used for decontamination of radioactive equipment, has been associated with increased migration of radionuclides into local groundwaters at some radwaste disposal sites. It has been proposed that predisposal thermal degradation of EDTA-containing aqueous solutions may reduce the potential for chelate-enhanced mobilization of radionuclides at these sites. Aqueous thermal degradation experiments with disodium EDTA have shown that the compound degrades rapidly at 200 0 C with an activation energy of 114.3 +- 7.87 kJ/mol, and forms the decomposition product methyliminodiacetic acid (MIDA). A comparison of the values for stability constants of transition metal and actinide complexes with EDTA, MIDA, and two other reported degradation products, indicates that the chelating efficiency of the degradation products is 6 to 22 orders of magnitude lower than that of EDTA at 25 0 C. It is concluded that aqueous thermal degradation should significantly reduce the overall chelating efficiency of EDTA-containing solutions

  14. Hepatobiliary delivery of polyaminopolycarboxylate chelates: Synthesis and characterization of a cholic acid conjugate of EDTA and biodistribution and imaging studies with its indium-111 chelate

    Energy Technology Data Exchange (ETDEWEB)

    Betebenner, D.A.; Carney, P.L.; Zimmer, A.M.; Kazikiewicz, J.M.; Bruecher, E.S.; Sherry, A.D.; Johnson, D.K. (Abbott Laboratories, Abbott Park, Illinois (USA))

    1991-03-01

    A conjugate in which the steroid nucleus of cholic acid was linked to EDTA via an 11-atom spacer was obtained by reacting the succinimidyl ester of cholic acid with the amine formed by reaction of a benzyl isothiocyanate derivative of EDTA with N-(tert-butoxycarbonyl)ethylenediamine and subsequent deprotection. Potentiometric titration studies with model complexes showed that the EDTA moiety retained the ability to form 1:1 chelates of high thermodynamic stability, although formation constants were some 3-4 log K units lower for complexes of the conjugate than for the analogous chelates with underivatized EDTA. A complex formed between the cholic acid-EDTA conjugate and 111InIII was clearly rapidly into the liver when injected iv into mice, with subsequent excretion from the liver into the gastrointestinal tract being complete within 1 h of injection. Radioscintigraphic imaging studies conducted in a rabbit given the 111In-labeled conjugate also showed early liver uptake followed by rapid clearance from the liver into the intestine, with good visualization of the gallbladder in images obtained at 20-25 min postinjection. It is concluded that conjugation to cholic acid provides a useful means for the hepatobiliary delivery of EDTA chelates that otherwise exhibit predominantly extracellular distribution and renal clearance.

  15. Hepatobiliary delivery of polyaminopolycarboxylate chelates: Synthesis and characterization of a cholic acid conjugate of EDTA and biodistribution and imaging studies with its indium-111 chelate

    International Nuclear Information System (INIS)

    Betebenner, D.A.; Carney, P.L.; Zimmer, A.M.; Kazikiewicz, J.M.; Bruecher, E.S.; Sherry, A.D.; Johnson, D.K.

    1991-01-01

    A conjugate in which the steroid nucleus of cholic acid was linked to EDTA via an 11-atom spacer was obtained by reacting the succinimidyl ester of cholic acid with the amine formed by reaction of a benzyl isothiocyanate derivative of EDTA with N-(tert-butoxycarbonyl)ethylenediamine and subsequent deprotection. Potentiometric titration studies with model complexes showed that the EDTA moiety retained the ability to form 1:1 chelates of high thermodynamic stability, although formation constants were some 3-4 log K units lower for complexes of the conjugate than for the analogous chelates with underivatized EDTA. A complex formed between the cholic acid-EDTA conjugate and 111InIII was clearly rapidly into the liver when injected iv into mice, with subsequent excretion from the liver into the gastrointestinal tract being complete within 1 h of injection. Radioscintigraphic imaging studies conducted in a rabbit given the 111In-labeled conjugate also showed early liver uptake followed by rapid clearance from the liver into the intestine, with good visualization of the gallbladder in images obtained at 20-25 min postinjection. It is concluded that conjugation to cholic acid provides a useful means for the hepatobiliary delivery of EDTA chelates that otherwise exhibit predominantly extracellular distribution and renal clearance

  16. Influence of multi-step washing using Na2EDTA, oxalic acid and phosphoric acid on metal fractionation and spectroscopy characteristics from contaminated soil.

    Science.gov (United States)

    Wei, Meng; Chen, Jiajun

    2016-11-01

    A multi-step soil washing test using a typical chelating agent (Na 2 EDTA), organic acid (oxalic acid), and inorganic weak acid (phosphoric acid) was conducted to remediate soil contaminated with heavy metals near an arsenic mining area. The aim of the test was to improve the heavy metal removal efficiency and investigate its influence on metal fractionation and the spectroscopy characteristics of contaminated soil. The results indicated that the orders of the multi-step washing were critical for the removal efficiencies of the metal fractions, bioavailability, and potential mobility due to the different dissolution levels of mineral fractions and the inter-transformation of metal fractions by XRD and FT-IR spectral analyses. The optimal soil washing options were identified as the Na 2 EDTA-phosphoric-oxalic acid (EPO) and phosphoric-oxalic acid-Na 2 EDTA (POE) sequences because of their high removal efficiencies (approximately 45 % for arsenic and 88 % for cadmium) and the minimal harmful effects that were determined by the mobility and bioavailability of the remaining heavy metals based on the metal stability (I R ) and modified redistribution index ([Formula: see text]).

  17. The difference of 17% ethylenediaminetetraacetic acid irrigation material contact time of 60 seconds and 30 seconds toward of cleanliness of apical third root canal wall

    Directory of Open Access Journals (Sweden)

    Yunita Wijaya

    2011-11-01

    Full Text Available Root canal preparation is one important step in endodontic treatment, involves the cleaning and the shaping of the root canal debris. Root canal cleaning effectiveness depends on the preparation bio-mechanical and irrigation. Purpose of this study was to evaluate the cleanliness of apical third of root canal wall from of debris, with the contact time of 17% EDTA irrigation material for 60 seconds and 30 seconds after root canal preparation using rotary NiTi instruments. This quasi-experimental study was carried out invitro, with random sampling technique. The sample used was 20 central maxillary incisors that have been extracted and divided into two experimental groups of 10 teeth each. The results were analyzed using student t statistics, showed that the average value of the debris of the two groups differed significantly. The contact time of 60 seconds of 17% EDTA showed cleaner root canal than the 30 seconds. The conclusion of this study was there were the differences of the cleanliness of apical third of the root canal with the 60 seconds contact time of 17% EDTA irrigation materials than 30 seconds contact time.

  18. Toxicity Thresholds Based on EDTA Extractable Nickel and Barley Root Elongation in Chinese Soils

    Directory of Open Access Journals (Sweden)

    Guangyun Zhu

    2018-04-01

    Full Text Available The uncertainty in the risk assessment of trace metal elements in soils when total metal contents are used can be decreased by assessing their availability and/or extractability when the soils have a high background value or different sources of trace metal elements. In this study, the added water-soluble nickel (Ni toxicity to barley root elongation was studied in 17 representative Chinese soil samples with and without artificial rainwater leaching. The extractability of added Ni in soils was estimated by three sequential extractions with ethylenediaminetetraacetic acid (EDTA. The results showed that the effective concentration of EDTA extractable Ni (EC50, which caused 50% inhibition of barley root elongation, ranged from 46 to 1019 mg/kg in unleached soils and 24 to 1563 mg/kg in leached soils. Regression models for EDTA extractable Ni and total Ni added to soils against soil properties indicated that EDTA extractable Ni was significantly correlated with the total Ni added to soils and that pH was the most important control factor. Regression models for toxicity thresholds based on EDTA extractable Ni against soil properties showed that soil citrate dithionate extractable Fe was more important than soil pH in predicting Ni toxicity. These results can be used to accurately assess the risk of contaminated soils with high background values and/or different Ni sources.

  19. Optimizing the molarity of a EDTA washing solution for saturated-soil remediation of trace metal contaminated soils

    International Nuclear Information System (INIS)

    Andrade, M.D.; Prasher, S.O.; Hendershot, W.H.

    2007-01-01

    Three experiments were conducted to optimize the use of ethylenediaminetetraacetic acid (EDTA) for reclaiming urban soils contaminated with trace metals. As compared to Na 2 EDTA (NH 4 ) 2 EDTA extracted 60% more Zn and equivalent amounts of Cd, Cu and Pb from a sandy loam. When successively saturating and draining loamy sand columns during a washing cycle, which submerged it once with a (NH 4 ) 2 EDTA wash and four times with deionised water, the post-wash rinses largely contributed to the total cumulative extraction of Cd, Co, Cr, Cu, Mn, Ni, Pb and Zn. Both the washing solution and the deionised water rinses were added in a 2:5 liquid to soil (L:S) weight ratio. For equal amounts of EDTA, concentrating the washing solution and applying it and the ensuing rinses in a smaller 1:5 L:S weight ratio, instead of a 2:5 L:S weight ratio, increased the extraction of targeted Cr, Cu, Ni, Pb and Zn. - A single EDTA addition is best utilised in a highly concentrated washing solution given in a small liquid to soil weight ratio

  20. Decomplexation efficiency and mechanism of Cu(II)-EDTA by H2O2 coupled internal micro-electrolysis process.

    Science.gov (United States)

    Zhou, Dongfang; Hu, Yongyou; Guo, Qian; Yuan, Weiguang; Deng, Jiefan; Dang, Yapan

    2016-12-29

    Internal micro-electrolysis (IE) coupled with Fenton oxidation (IEF) was a very effective technology for copper (Cu)-ethylenediaminetetraacetic acid (EDTA) wastewater treatment. However, the mechanisms of Cu 2+ removal and EDTA degradation were scarce and lack persuasion in the IEF process. In this paper, the decomplexation and removal efficiency of Cu-EDTA and the corresponding mechanisms during the IEF process were investigated by batch test. An empirical equation and the oxidation reduction potential (ORP) index were proposed to flexibly control IE and the Fenton process, respectively. The results showed that Cu 2+ , total organic carbon (TOC), and EDTA removal efficiencies were 99.6, 80.3, and 83.4%, respectively, under the proper operation conditions of iron dosage of 30 g/L, Fe/C of 3/1, initial pH of 3.0, Fe 2+ /H 2 O 2 molar ratio of 1/4, and reaction time of 20 min, respectively for IE and the Fenton process. The contributions of IE and Fenton to Cu 2+ removal were 91.2 and 8.4%, respectively, and those to TOC and EDTA removal were 23.3, 25.1, and 57, 58.3%, respectively. It was found that Fe 2+ -based replacement-precipitation and hydroxyl radical (•OH) were the most important effects during the IEF process. •OH played an important role in the degradation of EDTA, whose yield and productive rate were 3.13 mg/L and 0.157 mg/(L min -1 ), respectively. Based on the intermediates detected by GC-MS, including acetic acid, propionic acid, pentanoic acid, amino acetic acid, 3-(diethylamino)-1,2-propanediol, and nitrilotriacetic acid (NTA), a possible degradation pathway of Cu-EDTA in the IEF process was proposed. Graphical abstract The mechanism diagram of IEF process.

  1. The effect of laterally positioned flap-revised technique and 24% ethylenediaminetetraacetic acid root conditioning on root coverage: A case report

    Directory of Open Access Journals (Sweden)

    Jagmohan Singh

    2014-07-01

    Full Text Available Complete root coverage is considered the true goal of treatment of gingival recession defects because only complete coverage assures recovery from the hypersensitivity and esthetic defects associated with recession areas. Previous studies have shown that the laterally positioned flap (LPF technique or root surface biomodification yields a higher percentage of complete root coverage upon gingival recession treatment. This article highlights the use of the laterally positioned pedicle flap-revised technique (LPFRT as a modification of the LPF technique, along with 24% EDTA gel as a root surface biomodification agent, in the management of localized gingival recession defects. Clinical examination revealed a Miller class II recession defect on the buccal aspect of the lower right central incisor, as well as the presence of aberrant frenum pull adjacent to the recession defect. The LPFRT, together with 24% EDTA gel, was speculated to cover the gingival recession defect. The frenectomy, along with periosteal fenestration, was planned simultaneously with LPFRT. After 6 months of therapy, the clinical condition was stable with complete root coverage and satisfactory healing of the gingival tissues at both the donor and recipient sites with no signs of inflammation.

  2. Scanning electron microscopic evaluation of efficacy of 17% Ethylenediaminetetraacetic acid and chitosan for smear layer removal with ultrasonics: An In vitro study

    Directory of Open Access Journals (Sweden)

    Aradhana Babu Kamble

    2017-01-01

    Full Text Available Introduction: The main aim of root canal treatment is cleaning, shaping and then obturating three dimensionally to prevent reinfection. This includes chemicomechanical cleansing by instrumentation and the use of irrigating solutions. Therefore, the purpose of this study was to compare the smear layer removal from root canal dentine subjected to two root canal irrigants, 17% EDTA and 0.2% Chitosan, a new irrigant using Scanning Electron Microscope. Methodology: 40 single rooted premolars were decoronated followed by instrumentation with I Race files and intermediate irrigation with 3% sodium hypochlorite and activation with ultrasonics. Then the samples were longitudinally sectioned and place in the respective test solutions and their controls for 5 minutes. Scanning Electron Microscopic evaluation was further carried out. Results: The results of the present study indicates that the Chitosan which was proved effective in removing smear layer. Conclusion: A moderate concentration of 0.2% chitosan removes the smear layer with greater efficiency.

  3. 1064-nm Nd:YAG and 980-nm Diode Laser EDTA Agitation on the Retention of an Epoxy-Based Sealer to Root Dentin.

    Science.gov (United States)

    Macedo, Helena Suleiman de; Messias, Danielle Cristine Furtado; Rached-Júnior, Fuad Jacob; Oliveira, Ligia Teixeira de; Silva-Sousa, Yara Teresinha Correa; Raucci-Neto, Walter

    2016-01-01

    Root canal irrigants are used to minimize the negative effects of smear layer on endodontic sealer retention. The aim of this study was to evaluate the efficacy of agitation of 17% ethylenediaminetetraacetic acid (EDTA) with ultrasonic, 1064-nm Nd:YAG and 980-nm diode laser on the retention of an epoxy-based sealer to the root canal walls. Forty single-rooted bovine teeth were instrumented with ProTaper rotary system and divided into four groups according to the final irrigation protocol (n = 10): (1) 17% EDTA (control); (2) 17% EDTA with 50-s ultrasonic agitation; (3) 17% EDTA with 50-s diode laser (2-W) agitation; and (4) 17% EDTA with 50-s Nd:YAG (1.5-W) laser agitation. After endodontic filling with gutta-percha F5 master cone and Sealer 26, the roots were sectioned at the cervical, middle, and apical root thirds to obtain 1.5-mm slices. Push-out tests were performed using a universal testing machine at a 1 mm/min crosshead speed. Data were analyzed using two-way ANOVA and Tukey's tests (α=0.05). Apical root thirds had significant higher retention values than cervical and middle thirds (p diode laser presented the highest retention values and was significantly different from EDTA with ultrasonic agitation and EDTA only (p diode laser EDTA agitation enhanced the retention of the epoxy-based sealer to the root canal walls compared with that due to EDTA only or EDTA with ultrasonic agitation.

  4. A method for determination of Sr-90 Y-90 by using EDTA and ion-exchangers-Applications to the determination of those radioisotopes in milk

    International Nuclear Information System (INIS)

    Silva, C.M.; Lima, F.W. de.

    1987-05-01

    Amethod in which the complex of ethylenediaminetetraacetic acid (EDTA) with yttrium, used in conjunction with ion-exchangers for determination of Sr-90 is described. The method was to the determination of concentration of Sr-90 in milk, avoiding, in this way, protein elimination by acid precipitation or by evaporation of milk and ashing the residue. Analysis of samples of milk from various places in the state of Sao Pa ulo, Brazil, were carried out and the results are reported. Values found are much lower than maximum permissible concentration. (Author) [pt

  5. Dissolution of root canal sealers in EDTA and NaOCl solutions.

    Science.gov (United States)

    Keleş, Ali; Köseoğlu, Mustafa

    2009-01-01

    Solutions of ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite (NaOCl) have been used as canal irrigants in endodontic treatment. The authors conducted a study to compare the ability of these solutions to dissolve sealers. The authors assessed the solubility of six sealers-calcium hydroxide, polyketone, zinc oxide-eugenol, silicone and two epoxy resins-in EDTA and two concentrations of NaOCl (2.5 percent and 5.0 percent). They immersed standardized samples (n = 5) of each sealer for two minutes and 10 minutes. They obtained the mean values of sealer dissolution in solutions by calculating the difference between the original preimmersion and postimmersion weights to determine the amount of sealer removed. They compared the values via factorial analysis of variance. They analyzed differences between the six sealers with respect to their solubility in EDTA or NaOCl solutions at two minutes and 10 minutes by using a one-way analysis of variance (P Polyketone and calcium hydroxide-based sealers were the most soluble sealers (P < .05). The results of this study indicate that during nonsurgical endodontic re-treatment, EDTA and NaOCl solutions used for removing smear layer aided in the retreatment by dissolving some root canal sealers.

  6. Synthesis, Characterization, and Magnetic Properties of Pure and EDTA-Capped NiO Nanosized Particles

    Directory of Open Access Journals (Sweden)

    H. T. Rahal

    2017-01-01

    Full Text Available The effect of ethylenediaminetetraacetic acid (EDTA as a capping agent on the structure, morphology, optical, and magnetic properties of nickel oxide (NiO nanosized particles, synthesized by coprecipitation method, was investigated. Nickel chloride hexahydrate and sodium hydroxide (NaOH were used as precursors. The resultant nanoparticles were characterized by X-ray diffraction (XRD, transmission electron microscopy (TEM, and scanning electron microscopy (SEM. XRD patterns showed that NiO have a face-centered cubic (FCC structure. The crystallite size, estimated by Scherrer formula, has been found in the range of 28–33 nm. It is noticed that EDTA-capped NiO nanoparticles have a smaller size than pure nanoparticles. Thus, the addition of 0.1 M capping agent EDTA can form a nucleation point for nanoparticles growth. The optical and magnetic properties were investigated by Fourier transform infrared spectroscopy (FTIR and UV-vis absorption spectroscopy (UV as well as electron paramagnetic resonance (EPR and magnetization measurements. FTIR spectra indicated the presence of absorption bands in the range of 402–425 cm−1, which is a common feature of NiO. EPR for NiO nanosized particles was measured at room temperature. An EPR line with g factor ≈1.9–2 is detected for NiO nanoparticles, corresponding to Ni2+ ions. The magnetic hysteresis of NiO nanoparticles showed that EDTA capping recovers the surface magnetization of the nanoparticles.

  7. EDTA functionalized magnetic nanoparticle as a multifunctional adsorbent for Congo red dye from contaminated water

    Science.gov (United States)

    Sahoo, Jitendra Kumar; Rath, Juhi; Dash, Priyabrat; Sahoo, Harekrushna

    2017-05-01

    The present work reports the applicability of magnetite iron nanoparticles (Fe3O4) functionalized with ethylenediaminetetraacetic acid (EDTA) as an efficient adsorbent for the removal of Congo red (CR) dye from contaminated water. Magnetic nanoparticles (Fe3O4) are prepared by chemical precipitation method in which Fe2+ and Fe3+ salt from aqueous solution were reacted in presence of ammonia solution. The surface of Fe3O4 nanoparticle was first coated with (3-aminopropyl) triethoxy silane (APTES) by a salinization reaction and then linked with EDTA via reaction between -NH2 and -COOH to form well dispersed surface functionalised biocompatible magnetic nanoparticles. The obtained EDTA functionalized magnetic nanoparticles are characterized in terms of their morphological, XRD, BET surface area analysis, Fourier transform infrared spectroscopy (FT-IR) and Vibrating sample magnetometer (VSM). The adsorption of CR on Fe3O4-APTES-EDTA nanocomposite corresponds well to the Langmuir model and the Freundlich model respectively. The adsorption processes for CR followed the pseudo-second-order model.

  8. Oxidative stability of fish and algae oils containing long-chain polyunsaturated fatty acids in bulk and in oil-in-water emulsions

    DEFF Research Database (Denmark)

    Frankel, E.N.; Satue-Gracia, T.; Meyer, Anne Boye Strunge

    2002-01-01

    from algae are unusually stable to oxidation, Addition of ethylenediaminetetraacetic acid (EDTA) prevented oxidation of both fish and algal oil emulsions without added iron and at low iron:EDTA molar concentrations. EDTA, however, promoted the oxidation of the corresponding emulsions that contained...... high iron:EDTA ratios. Therefore, to be effective as a metal chelator, EDTA must be added at molar concentrations higher than that of iron to inhibit oxidation of foods containing long-chain PUFA from either fish or algae and fortified with iron.......The oxidative stability of long-chain polyunsaturated fatty acid (PUFA) and docosahexaenoic acid (DHA)-containing fish and algae oils varies widely according to their fatty acid composition, the physical and colloidal states of the lipids, the contents of tocopherols and other antioxidants...

  9. Effect of EDTA Conditioning and Carbodiimide Pretreatment on the Bonding Performance of All-in-One Self-Etch Adhesives

    Directory of Open Access Journals (Sweden)

    Shipra Singh

    2015-01-01

    Full Text Available Objective. This study evaluated the effect of ethylenediaminetetraacetic acid (EDTA conditioning and carbodiimide (EDC pretreatment on the shear bond strength of two all-in-one self-etch adhesives to dentin. Methods. Flat coronal dentin surfaces were prepared on one hundred and sixty extracted human molars. Teeth were randomly divided into eight groups according to two different self-etch adhesives used [G-Bond and OptiBond-All-In-One] and four different surface pretreatments: (a adhesive applied following manufacturer’s instructions; (b dentin conditioning with 24% EDTA gel prior to application of adhesive; (c EDC pretreatment followed by application of adhesive; (d application of EDC on EDTA conditioned dentin surface followed by application of adhesive. Composite restorations were placed in all the samples. Ten samples from each group were subjected to immediate and delayed (6-month storage in artificial saliva shear bond strength evaluation. Data collected was subjected to statistical analysis using three-way ANOVA and post hoc Tukey’s test at a significance level of p<0.05.  Results and Conclusion. EDTA preconditioning as well as EDC pretreatment alone had no significant effect on the immediate and delayed bond strengths of either of the adhesives. However, EDC pretreatment on EDTA conditioned dentin surface resulted in preservation of resin-dentin bond strength of both adhesives with no significant fall over six months.

  10. Effects of lactoferrin, phytic acid, and EDTA on oxidation in two food emulsions enriched with long-chain polyunsaturated fatty acids

    DEFF Research Database (Denmark)

    Nielsen, Nina Skall; Petersen, A.; Meyer, Anne S.

    2004-01-01

    The influence of the addition of metal chelators on oxidative stability was studied in a milk drink and in a mayonnaise system containing highly polyunsaturated lipids. Milk drinks containing 5% (w/w) of specific structured lipid were supplemented with lactoferrin (6-24 M) and stored at 2 C for up...... to 9 weeks. Mayonnaise samples with 16% fish oil and 64% rapeseed oil (w/w) were supplemented with either lactoferrin (8-32 M), phytic acid (16-124 M), or EDTA (16-64 M) and were stored at 20 C for up to 4 weeks. The effect of the metal chelators was evaluated by determination of peroxide values...

  11. Enhanced sensitivity to the lethal and mutagenic effects of photosensitizing action of chlorpromazine in ethylenediaminetetraacetate-treated Escherichia coli

    International Nuclear Information System (INIS)

    Yonei, S.; Todo, T.

    1982-01-01

    Ethylenediaminetetraacetate (EDTA) treatment of Escherichia coli H/r30 (Arg - ) enhanced cell sensitivity to the lethal and mutagenic effects of the photosensitizing action of chlorpromazine (CPZ). The most obvious effect of EDTA on the fluence-survival curve was an elimination of the shoulder. In the absence of EDTA, CPZ plus near-UV radiation did not induce the reversion from arginine-auxotroph to autotroph of E. coli H/r30. However, when EDTA (5 mM)-treated cells were subjected to CPZ plus near-UV radiation, the induced reversion frequency increased with time of irradiation. It is concluded that the enhanced penetration of CPZ into E. coli cells by EDTA facilitates the drug binding to DNA within the cells upon near-UV irradiation and that this is the cause for the enhanced photosensitized lethal and mutagenic effects of CPZ. (author)

  12. A new Pb{sup II}(ethylenediaminetetraacetate) coordination polymer with a two-dimensional layer structure

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, D., E-mail: iamzd@hpu.edu.cn; Zhang, R. H.; Li, F. F. [Henan Polytechnic University, Department of Physics and Chemistry (China)

    2016-12-15

    A new Pb{sup II}−edta{sup 4–} coordination polymer, Pb{sub 2}(edta)(H{sub 2}O){sub 0.76} (edta{sup 4–} = ethylenediaminetetraacetate) was synthesized under hydrothermal condition. Single crystal X-ray analysis reveals that it represents a novel two-dimensional (2D) Pb{sup 2+}–edta{sup 4–} layer structure with a (4,8{sup 2})-topology. Each edta{sup 4–} ligand employs its four carboxylate O and two N atoms to chelate one Pb{sup II} atom (hexa-coordinated) and connects five Pb{sup II} atoms (ennea-coordinated) via its four carboxylate groups to form 2D layer framework. Adjacent layers are packed into the overall structure through vander Waals interactions.

  13. Laser Monitoring Of Phytoextraction Enhancement Of Lead Contaminated Soil Adopting EDTA And EDDS

    Science.gov (United States)

    Hassan, M.; Abdelhamied, M.; Hanafy, A. H.; Fantoni, R.; Harith, M. A.

    2011-09-01

    Removal of heavy metals (HMs) such as Pb from soil, wastewater, and air is essential for environment and human health. Phytoremediation is a well established technology based on the use of certain green plants for contaminants removal from soil, wastewater as well as air. Scented geranium, Pelargonium zonal, is a flowering plant recently used in HMs removal from contaminated soil. In the present work, EDTA (ethylenediaminetetraacetic acid) and EDDS (S, S-ethylenediaminedisuccinic acid) were used as chemical assistants providing higher Pb availability for extraction by plant roots. Lead was artificially added to the planting media, peatmoss, at different concentrations. Laser induced breakdown spectroscopy (LIBS) was used to follow up Pb relative concentrations in peatmoss as well as plant shoots, at different sampling times during the experiment period. Laser induced chlorophyll fluorescence (LICF), has been also used to evaluate chlorophyll formation and photosynthetic apparatus status in geranium plants. Such measurements were performed on geranium plants grown under various Pb levels, as well as EDTA and EDDS combinations. The combined effect of EDTA and EDDS was found to enhance Pb extraction with time. Good correlation was found between LICF results and chlorophyll (a) (Chl.a) concentrations in plant tissues extracted by chemical analysis.

  14. Comparison of the ability of organic acids and EDTA to enhance the phytoextraction of metals from a multi-metal contaminated soil.

    Science.gov (United States)

    Kim, Sung-Hyun; Lee, In-Sook

    2010-02-01

    Chelates have been shown to enhance the phytoextraction of metal from contaminated soil. In this study, we evaluated the ability of chelates to enhance the phytoextraction of metals by barnyard grass (Echinochloa crus-galli) from soils contaminated with multiple metals. The results revealed that EDTA increased the ability of barnyard grass to take up Cd, Cu and Pb, but that it resulted in increased soil leaching. Conversely, citric acid induced the removal of Cd, Cu and Pb from soil without increasing the risk of leaching. Furthermore, E.crus-galli showed no signs of phytotoxicity in response to treatment with citric acid, whereas its shoot growth decreased in response to treatment with EDTA (p acid is a good agent for the enhancement of the phytoextraction of metals.

  15. Biochemical effects of ethylene diamine tetra-acetic acid (EDTA) on ...

    African Journals Online (AJOL)

    mamus

    2016-04-13

    Apr 13, 2016 ... fresh weight, level of lipid per oxidation, alkaline phosphatase, acid phosphatase, super oxide dismutase ... Its effects on plants are manifested by inhibition of the normal uptake and utilization of mineral nutrients (Liu et al., 2003). ... reactive oxygen species (ROS) indirectly by production of disturbances in ...

  16. Comparative study of conventional PAH and inulin clearance and slope clearance of 131I-o-hippuric acid and 51Cr-EDTA

    International Nuclear Information System (INIS)

    Berghaeuser, H.

    1981-01-01

    ''Classic'' PAH and inulin clearance were determined in 81 patients with renal anomalies or renal diseases of different genesis and severity. In addition, radioisotope nephrography was carried out after administration of I-131-o-hippuric acid, followed by administration of Cr-51-EDTA. The activity decrease was recorded by a sensor located over the patient's right shoulder; the activities of a serum sample and of the urine excreted after 21 or 31 min were measured, and the findings were compared with those of the classic method. The clearance data calculated on the basis of the soulder measurements were hardly compatible with those of the conventional method in the case of 131 I-o-hippuric acid (r=0.54) and totally incompatible in the case of 51 Cr-EDTA. This means that the method described by Oberhausen is the only accurate method available for a quantitative assessment of the renal function on the basis of measurements of the activity decrease in the body. The activity of urine excreted after 31 min ( 131 I-hippuric acid:r=0.992, 51 Cr-EDTA:r=0.79) is a sufficiently accurate parameter although it is inaccurate at PAH clearance, values > 130 ml/min and inulin clearance values > 30 ml/min. Of the many parameters of radioisotope nephrogram curves, in the case of 131 I-o-hippuric acid only the parameters related to the ascent between 48 and 120 sec or to the secant ascent yield sufficient quantitative information for certain functional regions (r=0.9 resp. r=0.93). In the case of 51 Cr-EDTA, semiquantitative information on the renal function can be obtained by constructing secants on the nephrogram curves (r=0.7 resp. r=0.72). Here as in the case of 131 I-hippuric acid, the contribution of each kidney can be determined individually from the functional analysis of both kidneys. (orig.) [de

  17. Influence of irradiation and Caf EDTA on the metabolism of nucleus acid of the different radiosensitive crops

    International Nuclear Information System (INIS)

    Song Daojun

    1997-01-01

    Irradiation and damage repair inhibitor (Caf and EDTA) post-treatment had a substantial effect on DNA and RNA content and DNA self-repairing synthesis. The experiment results showed the content of DNA and RNA increased with increase doses in the seedling hypocotyl of soybean and rape, Caf and EDTA post-treatment made them further higher than that of H 2 O-treatment. Meanwhile, the content of DNA and RNA in hypocotyl of soybean seedling was more than that of rape. Radioactive 3 H-TdR incorporation experiment showed γ-ray irradiated seeds induced unshunted DNA synthesis in the primary cultivated seeds of two crops, which was inhibited by Caf, EDTA and C + E post-treatment in the order of C + E>Caf>EDTA. Experiment also revealed irradiation and repair repressor post-treatment affected normal shunted DNA synthesis, postponed the cycle of cell division

  18. Biodegradation of PuEDTA and Impacts on Pu Mobility

    International Nuclear Information System (INIS)

    Xun, Luying; Bolton, Jr. Harvey

    2002-01-01

    This project, by Dr. Xun, supports work at PNNL (Bolton) regarding plutonium mobility in the subsurface. Ethylenediaminetetraacetate (EDTA) is a chelating agent that can increase the mobility of radionuclides and heavy metals in groundwater. Biodegradation of EDTA can decrease the enhanced mobility. The overall objective is to understand how microbial degradation affects Plutonium-EDTA transport in the environment and the specific objective of this component is to understand how microorganisms degrade EDTA. A chelating degrading bacterium BNC1 can use EDTA and nitrilotriacetate (NTA) as sole carbon and nitrogen sources. A gene cluster responsible for both EDTA and NTA degradation has been cloned and characterized (1,2). The same enzymes are used to degrade both compounds except that additional enzymes are required for EDTA degradation. Since the enzymes are located inside cells, EDTA and NTA must be transported into cells for degradation. For the first funding year, we have focused on how EDTA and NTA are transported into BNC1 cells. The EDTA-degrading gene cluster also contains genes encoding a hypothetical ABC-type transporter. We first demonstrated that the transporter genes and EDTA monooxygenase gene (emoA) were co-transcribed by RT-PCR, suggesting that the genes are involved in EDTA transport. We then characterized one of the gene product EppA. Using recombinant EppA purified from Escherichia coli, we have shown that EppA binds several metal:EDTA complexes by fluorescence techniques. In addition, EppA is shown to bind Mg:NTA, Ca:NTA and Fe(III):NTA but not free NTA

  19. Histological analysis of effects of 24% EDTA gel for nonsurgical treatment of periodontal tissues.

    Science.gov (United States)

    de Vasconcellos, Luana Marotta Reis; Ricardo, Lucilene Hernandes; Balducci, Ivan; de Vasconcellos, Luis Gustavo Oliveira; Carvalho, Yasmin Rodarte

    2006-12-01

    The aim of this study was to investigate, by means of histological and histomorphometric analysis, the effects of 24% ethylenediaminetetraacetic acid (EDTA) gel in periodontal tissue when used in combination with conventional periodontal treatment. Periodontitis was induced in the 2nd upper left permanent molars of 45 male Wistar rats by means of ligature. After 5 weeks, this was removed and debridement was performed. The animals were then randomly divided into 3 groups; group 1: mechanical treatment, group 2: mechanical treatment and EDTA gel application for 2 min, and group 3: mechanical treatment and placebo gel application for 2 min. After the treatment, rinsing was done with 0.9% saline solution for 1 min in all cases, followed by root notching in the deepest part of the pocket. After 4, 10, and 28 days the animals were sacrificed. The averages obtained were evaluated by means of test two-way analysis of variance (ANOVA) and Tukey statistical tests (P 24% EDTA gel did not interfere with periodontal tissue repair when used in combination with conventional periodontal treatment.

  20. Electrospun polyacrylonitrile nanofibers functionalized with EDTA for adsorption of ionic dyes

    Science.gov (United States)

    Chaúque, Eutilério F. C.; Dlamini, Langelihle N.; Adelodun, Adedeji A.; Greyling, Corinne J.; Ngila, J. Catherine

    2017-08-01

    The manipulation of nanofibers' surface chemistry could enhance their potential application toward the removal of ionic dyes in wastewater. For this purpose, surface modification of electrospun polyacrylonitrile (PAN) nanofibers with ethylenediaminetetraacetic acid (EDTA) and ethylenediamine (EDA) crosslinker was experimented. The functionalized EDTA-EDA-PAN nanofibers were characterized using Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) technique. The impregnation of EDA and EDTA chelating agents on the surface of PAN changed the distribution of nanofibers as proximity is increased (accompanied by reduced softness), but the nanofibrous structure of the pristine PAN nanofibers was not substantially altered. Adsorption equilibrium studies were performed with Freundlich, Langmuir and Temkin isotherm models with the former providing better correlation to the experimental data. The modified PAN nanofibers showed efficient sorption of methyl orange (MO) and reactive red (RR) from aqueous synthetic samples, evinced by the maximum adsorption capacities (at 25 °C) of 99.15 and 110.0 mg g-1, respectively. The fabricated nanofibers showed appreciable removal efficiency of the target dye sorptives from wastewater. However, the presence of high metal ions content affected the overall extraction of dyes from wastewater due to the depletion of the adsorbent's active adsorptive sites.

  1. Chromatographic separation of europium and gadolinum mixtures by ethylenediaminedisuccinic acid

    International Nuclear Information System (INIS)

    Kolleganov, M.Yu.; Nazarov, P.P.; Martynenko, L.I.; Mtrofanova, N.D.; Spitsyn, V.I.

    1985-01-01

    Comparative investigation of chromatographic separation of impurities of Eu and Gd micro- and macroquantities by means of ethylenediaminedisuccinic (EDDS) and ethylene-diaminetetraacetic (EDTA) acids is performed. It is shown that EDTA is a sufficiently effective agent for separation of this pair of elements. The values of EU and Gd separation coefficients obtained in experiments with RE microquantities are not realized at Eu-Gd, macroquantities separation which probably is connected with the influence of polymerization processes in the systems containing RE and EDDS complexes

  2. Anaerobic Biotransformation and Mobility of Pu and Pu-EDTA

    International Nuclear Information System (INIS)

    Bolton, H. Jr.; Rai, D.; Xun, L.

    2005-01-01

    The complexation of radionuclides (e.g., plutonium (Pu) and 60 Co) by codisposed ethylenediaminetetraacetate (EDTA) has enhanced their transport in sediments at DOE sites. Our previous NABIR research investigated the aerobic biodegradation and biogeochemistry of Pu(IV)-EDTA. Plutonium(IV) forms stable complexes with EDTA under aerobic conditions and an aerobic EDTA degrading bacterium can degrade EDTA in the presence of Pu and decrease Pu mobility. However, our recent studies indicate that while Pu(IV)-EDTA is stable in simple aqueous systems, it is not stable in the presence of relatively soluble Fe(III) compounds (i.e., Fe(OH) 3 (s)--2-line ferrihydrite). Since most DOE sites have Fe(III) containing sediments, Pu(IV) in likely not the mobile form of Pu-EDTA in groundwater. The only other Pu-EDTA complex stable in groundwater relevant to DOE sites would be Pu(III)-EDTA, which only forms under anaerobic conditions. Research is therefore needed in this brand new project to investigate the biotransformation of Pu and Pu-EDTA under anaerobic conditions. The biotransformation of Pu and Pu-EDTA under various anaerobic regimes is poorly understood including the reduction kinetics of Pu(IV) to Pu(III) from soluble (Pu(IV)-EDTA) and insoluble Pu(IV) as PuO2(am) by metal reducing bacteria, the redox conditions required for this reduction, the strength of the Pu(III)-EDTA complex, how the Pu(III)-EDTA complex competes with other dominant anoxic soluble metals (e.g., Fe(II)), and the oxidation kinetics of Pu(III)-EDTA. Finally, the formation of a stable soluble Pu(III)-EDTA complex under anaerobic conditions would require degradation of the EDTA complex to limit Pu(III) transport in geologic environments. Anaerobic EDTA degrading microorganisms have not been isolated. These knowledge gaps preclude the development of a mechanistic understanding of how anaerobic conditions will influence Pu and Pu-EDTA fate and transport to assess, model, and design approaches to stop Pu

  3. Examination of blood-brain barrier permeability in dementia of the Alzheimer type with [68Ga]EDTA and positron emission tomography

    International Nuclear Information System (INIS)

    Schlageter, N.L.; Carson, R.E.; Rapoport, S.I.

    1987-01-01

    Positron emission tomography with [ 68 Ga]ethylenediaminetetraacetic acid ([ 68 Ga]EDTA) was used to examine the integrity of the blood-brain barrier (BBB) in five patients with dementia of the Alzheimer type and in five healthy age-matched controls. Within a scanning time of 90 min, there was no evidence that measurable intravascular tracer entered the brain in either the dementia or the control group. An upper limit for the cerebrovascular permeability-surface area product of [68Ga]EDTA was estimated as 2 X 10(-6) s-1 in both groups. The results provide no evidence for breakdown of the BBB in patients with dementia of the Alzheimer type

  4. Examination of blood-brain barrier permeability in dementia of the Alzheimer type with [68Ga]EDTA and positron emission tomography.

    Science.gov (United States)

    Schlageter, N L; Carson, R E; Rapoport, S I

    1987-02-01

    Positron emission tomography with [68Ga]ethylenediaminetetraacetic acid ([68Ga]EDTA) was used to examine the integrity of the blood-brain barrier (BBB) in five patients with dementia of the Alzheimer type and in five healthy age-matched controls. Within a scanning time of 90 min, there was no evidence that measurable intravascular tracer entered the brain in either the dementia or the control group. An upper limit for the cerebrovascular permeability-surface area product of [68Ga]EDTA was estimated as 2 X 10(-6) s-1 in both groups. The results provide no evidence for breakdown of the BBB in patients with dementia of the Alzheimer type.

  5. Examination of blood-brain barrier permeability in dementia of the Alzheimer type with (68Ga)EDTA and positron emission tomography

    Energy Technology Data Exchange (ETDEWEB)

    Schlageter, N.L.; Carson, R.E.; Rapoport, S.I.

    1987-02-01

    Positron emission tomography with (/sup 68/Ga)ethylenediaminetetraacetic acid ((/sup 68/Ga)EDTA) was used to examine the integrity of the blood-brain barrier (BBB) in five patients with dementia of the Alzheimer type and in five healthy age-matched controls. Within a scanning time of 90 min, there was no evidence that measurable intravascular tracer entered the brain in either the dementia or the control group. An upper limit for the cerebrovascular permeability-surface area product of (68Ga)EDTA was estimated as 2 X 10(-6) s-1 in both groups. The results provide no evidence for breakdown of the BBB in patients with dementia of the Alzheimer type.

  6. The Ability of PAS, Acetylsalicylic Acid and Calcium Disodium EDTA to Protect Against the Toxic Effects of Manganese on Mitochondrial Respiration in Gill of Crassostrea virginica.

    Science.gov (United States)

    Crawford, Sherine; Davis, Kiyya; Saddler, Claudette; Joseph, Jevaun; Catapane, Edward J; Carroll, Margaret A

    2011-01-01

    Manganese (Mn) is an essential metal that at excessive levels in brain causes Manganism, a condition similar to Parkinson's disease. Previously we showed that Mn had a neurotoxic effect on the dopaminergic, but not serotonergic, innervation of the lateral ciliated cells in the gill of the Eastern Oyster, Crassostrea virginica. While the mechanism of action of Mn toxicity is not completely understood, studies suggest that Mn toxicity may involve mitochondrial damage and resulting neural dysfunction in the brain's dopaminergic system. In this study we utilized micro-batch chambers and oxygen probes to measure oyster gill mitochondrial respiration in the presence of Mn and potential Mn blockers. The addition of Mn to respiring mitochondria caused a dose dependent decrease in mitochondrial O(2) consumption. Pretreating mitochondria with calcium disodium EDTA (caEDTA), p aminosalicylic acid (PAS) or acetylsalicylic acid (ASA) before Mn additions, provided full protection against the toxic effects of Mn. While mitochondrial pretreatment with any of the 3 drugs effectively blocked Mn toxicity, none of the drugs tested was able to reverse the decrease in mitochondrial O(2) consumption seen in Mn treated mitochondria. The study found that high levels of Mn had a toxic effect on gill mitochondrial O(2) consumption and that this effect could be blocked by the drugs caEDTA, PAS and ASA. C. virginica continues to be a good model with which to investigate the mechanism that underlies manganese neurotoxcity and in the pharmacological study of drugs to treat or prevent Manganism.

  7. EDTA addition enhances bacterial respiration activities and hydrocarbon degradation in bioaugmented and non-bioaugmented oil-contaminated desert soils.

    Science.gov (United States)

    Al Kharusi, Samiha; Abed, Raeid M M; Dobretsov, Sergey

    2016-03-01

    The low number and activity of hydrocarbon-degrading bacteria and the low solubility and availability of hydrocarbons hamper bioremediation of oil-contaminated soils in arid deserts, thus bioremediation treatments that circumvent these limitations are required. We tested the effect of Ethylenediaminetetraacetic acid (EDTA) addition, at different concentrations (i.e. 0.1, 1 and 10 mM), on bacterial respiration and biodegradation of Arabian light oil in bioaugmented (i.e. with the addition of exogenous alkane-degrading consortium) and non-bioaugmented oil-contaminated desert soils. Post-treatment shifts in the soils' bacterial community structure were monitored using MiSeq sequencing. Bacterial respiration, indicated by the amount of evolved CO2, was highest at 10 mM EDTA in bioaugmented and non-bioaugmented soils, reaching an amount of 2.2 ± 0.08 and 1.6 ± 0.02 mg-CO2 g(-1) after 14 days of incubation, respectively. GC-MS revealed that 91.5% of the C14-C30 alkanes were degraded after 42 days when 10 mM EDTA and the bacterial consortium were added together. MiSeq sequencing showed that 78-91% of retrieved sequences in the original soil belonged to Deinococci, Alphaproteobacteria, Gammaproteobacteia and Bacilli. The same bacterial classes were detected in the 10 mM EDTA-treated soils, however with slight differences in their relative abundances. In the bioaugmented soils, only Alcanivorax sp. MH3 and Parvibaculum sp. MH21 from the exogenous bacterial consortium could survive until the end of the experiment. We conclude that the addition of EDTA at appropriate concentrations could facilitate biodegradation processes by increasing hydrocarbon availability to microbes. The addition of exogenous oil-degrading bacteria along with EDTA could serve as an ideal solution for the decontamination of oil-contaminated desert soils. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. Anaerobic Biotransformation and Mobility of Pu and of Pu-EDTA

    Energy Technology Data Exchange (ETDEWEB)

    Xun, Luying

    2009-11-20

    The enhanced mobility of radionuclides by co-disposed chelating agent, ethylenediaminetetraacetate (EDTA), is likely to occur only under anaerobic conditions. Our extensive effort to enrich and isolate anaerobic EDTA-degrading bacteria has failed. Others has tried and also failed. To explain the lack of anaerobic biodegradation of EDTA, we proposed that EDTA has to be transported into the cells for metabolism. A failure of uptake may contribute to the lack of EDTA degradation under anaerobic conditions. We demonstrated that an aerobic EDTA-degrading bacterium strain BNC1 uses an ABC-type transporter system to uptake EDTA. The system has a periplasmic binding protein that bind EDTA and then interacts with membrane proteins to transport EDTA into the cell at the expense of ATP. The bind protein EppA binds only free EDTA with a Kd of 25 nM. The low Kd value indicates high affinity. However, the Kd value of Ni-EDTA is 2.4 x 10^(-10) nM, indicating much stronger stability. Since Ni and other trace metals are essential for anaerobic respiration, we conclude that the added EDTA sequestrates all trace metals and making anaerobic respiration impossible. Thus, the data explain the lack of anaerobic enrichment cultures for EDTA degradation. Although we did not obtain an EDTA degrading culture under anaerobic conditions, our finding may promote the use of certain metals that forms more stable metal-EDTA complexes than Pu(III)-EDTA to prevent the enhanced mobility. Further, our data explain why EDTA is the most dominant organic pollutant in surface waters, due to the lack of degradation of certain metal-EDTA complexes.

  9. Anaerobic Biotransformation and Mobility of Pu and of Pu-EDTA

    International Nuclear Information System (INIS)

    Xun, Luying

    2009-01-01

    The enhanced mobility of radionuclides by co-disposed chelating agent, ethylenediaminetetraacetate (EDTA), is likely to occur only under anaerobic conditions. Our extensive effort to enrich and isolate anaerobic EDTA-degrading bacteria has failed. Others has tried and also failed. To explain the lack of anaerobic biodegradation of EDTA, we proposed that EDTA has to be transported into the cells for metabolism. A failure of uptake may contribute to the lack of EDTA degradation under anaerobic conditions. We demonstrated that an aerobic EDTA-degrading bacterium strain BNC1 uses an ABC-type transporter system to uptake EDTA. The system has a periplasmic binding protein that bind EDTA and then interacts with membrane proteins to transport EDTA into the cell at the expense of ATP. The bind protein EppA binds only free EDTA with a Kd of 25 nM. The low Kd value indicates high affinity. However, the Kd value of Ni-EDTA is 2.4 x 10 -10 nM, indicating much stronger stability. Since Ni and other trace metals are essential for anaerobic respiration, we conclude that the added EDTA sequestrates all trace metals and making anaerobic respiration impossible. Thus, the data explain the lack of anaerobic enrichment cultures for EDTA degradation. Although we did not obtain an EDTA degrading culture under anaerobic conditions, our finding may promote the use of certain metals that forms more stable metal-EDTA complexes than Pu(III)-EDTA to prevent the enhanced mobility. Further, our data explain why EDTA is the most dominant organic pollutant in surface waters, due to the lack of degradation of certain metal-EDTA complexes.

  10. Differential perturbed angular correlation: use of physico-chemical study of some hafnium complexes derivates of hydroxy acids and EDTA

    International Nuclear Information System (INIS)

    Silveira, J.G. da.

    1981-01-01

    Measures of quadrupolar interaction to nucleus level of the metal, in some hafnium complexes are presented, including the analysis by combustion, microanalysis, infrared spectroscopy and thermogravimetry. The hyperfine interaction parameters, the temperature effects and the thermal neutrons capture effects over the irradiated Hf Y (Y = EDTA) are also studied. (author)

  11. Effect of EDTA and Citric Acid on Phytoextraction of Copper and Zinc from a Naturally Contaminated Soil by Maize (Zea mays L. Cultivars

    Directory of Open Access Journals (Sweden)

    A. Taheripur

    2016-09-01

    Full Text Available Introduction: Mining and smelting activities have contributed to increasing levels of copper (Cu and zinc (Zn in soils around of Sarcheshmeh copper mine (Kerman, Iran. Soil chemical analysis showed that the available of Cu and Zn (extracted with DTPA-TEA were 260.1 and 9.2 mg kg-1 soil, respectively. Phytoextraction is one of the most popular and useful phytoremediation techniques for removal of heavy metals from polluted soils. For chemically-assisted phytoextraction, different chelating agents such as EDTA and citric acid are applied to soil to increase the availability of heavy metals in soil for uptake by plants. A pot experiment was conducted to elucidate the performance of chelating agents addition in improving phytoextraction of Cu and zinc Zn from a naturally contaminated soil by maize (Zea mays L. cultivars. Materials and Methods: A factorial experiment in a completely randomized design was carried out bythree factors of chelate type, chelate concentrations and maize cultivars with three replications in 2012 at ShahreKord University. Chelating agents were Ethylene Diamine Tetra Acetic Acid (EDTA and citric acid (CA. They were applied in concentration levels of 0, 0.75 and 1.5 mmole kg-1 soil with irrigation water. The three maize cultivars used were single cross 704 (SC-704, three v cross 647 (TVC-647, and single cross 677 (SC-677. The pots were 23 cm in diameter and 23 cm deep, and were filled with 4 kg of a silty loam, calcareous soil taken from the surface layer of Sarcheshmeh copper mine area. Maize plant s was grown under greenhouse conditions over 90 days. After the harvest, soil available Cu and Zn contents (extracted with DTPA-TEA were determined by atomic absorption spectrophotometry (AAS. Plant samples (shoot and root were dried for 48 h at 70ºC to determine their dry matter content (yield. Total Cu and Zn concentrations in root and shoot of maize were measured after digestion plant samples by AAS method. The shoot and root

  12. Assessing Glomerular Filtration in Small Animals Using [68Ga]DTPA and [68Ga]EDTA with PET Imaging.

    Science.gov (United States)

    Gündel, Daniel; Pohle, Ulrike; Prell, Erik; Odparlik, Andreas; Thews, Oliver

    2018-06-01

    Determining the glomerular filtration rate (GFR) is essential for clinical medicine but also for pre-clinical animal studies. Functional imaging using positron emission tomography (PET) allows repetitive almost non-invasive measurements. The aim of the study was the development and evaluation of easily synthesizable PET tracers for GFR measurements in small animals. Diethylenetriaminepentaacetic acid (DTPA) and ethylenediaminetetraacetic acid (EDTA) were labeled with Ga-68. The binding to blood cells and plasma proteins was tested in vitro. The distribution of the tracers in rats was analyzed by PET imaging and ex vivo measurements. From the time-activity-curve of the blood compartment (heart) and the total tracer mass excreted by the kidney, the GFR was calculated. These values were compared directly with the inulin clearance in the same animals. Both tracers did not bind to blood cells. [ 68 Ga]DPTA but not [ 68 Ga]EDTA showed strong binding to plasma proteins. For this reason, [ 68 Ga]DPTA stayed much longer in the blood and only 30 % of the injected dose was eliminated by the kidney within 60 min whereas the excretion of [ 68 Ga]EDTA was 89 ± 1 %. The calculated GFR using [ 68 Ga]EDTA was comparable to the measured inulin clearance in the same animal. Using [ 68 Ga]-DPTA, the measurements led to values which were 80 % below the normal GFR. The results also revealed that definition of the volume of interest for the blood compartment affects the calculation and may lead to a slight overestimation of the GFR. [ 68 Ga]EDTA is a suitable tracer for GFR calculation from PET imaging in small animals. It is easy to be labeled, and the results are in good accordance with the inulin clearance. [ 68 Ga]DTPA led to a marked underestimation of GFR due to its strong binding to plasma proteins and is therefore not an appropriate tracer for GFR measurements.

  13. Transport of Sr 2+ and SrEDTA 2- in partially-saturated and heterogeneous sediments

    Science.gov (United States)

    Pace, M. N.; Mayes, M. A.; Jardine, P. M.; McKay, L. D.; Yin, X. L.; Mehlhorn, T. L.; Liu, Q.; Gürleyük, H.

    2007-05-01

    Strontium-90 has migrated deep into the unsaturated subsurface beneath leaking storage tanks in the Waste Management Areas (WMA) at the U.S. Department of Energy's (DOE) Hanford Reservation. Faster than expected transport of contaminants in the vadose zone is typically attributed to either physical hydrologic processes such as development of preferential flow pathways, or to geochemical processes such as the formation of stable, anionic complexes with organic chelates, e.g., ethylenediaminetetraacetic acid (EDTA). The goal of this paper is to determine whether hydrological processes in the Hanford sediments can influence the geochemistry of the system and hence control transport of Sr 2+ and SrEDTA 2-. The study used batch isotherms, saturated packed column experiments, and an unsaturated transport experiment in an undisturbed core. Isotherms and repacked column experiments suggested that the SrEDTA 2- complex was unstable in the presence of Hanford sediments, resulting in dissociation and transport of Sr 2+ as a divalent cation. A decrease in sorption with increasing solid:solution ratio for Sr 2+ and SrEDTA 2- suggested mineral dissolution resulted in competition for sorption sites and the formation of stable aqueous complexes. This was confirmed by detection of MgEDTA 2-, MnEDTA 2-, PbEDTA 2-, and unidentified Sr and Ca complexes. Displacement of Sr 2+ through a partially-saturated undisturbed core resulted in less retardation and more irreversible sorption than was observed in the saturated repacked columns, and model results suggested a significant reservoir (49%) of immobile water was present during transport through the heterogeneous layered sediments. The undisturbed core was subsequently disassembled along distinct bedding planes and subjected to sequential extractions. Strontium was unequally distributed between carbonates (49%), ion exchange sites (37%), and the oxide (14%) fraction. An inverse relationship between mass wetness and Sr suggested that

  14. Extraction of heavy metals from contaminated soils using EDTA and HCl

    Directory of Open Access Journals (Sweden)

    Hatem Asel Gzar

    2015-01-01

    Full Text Available The present study examines the extraction of lead (Pb, cadmium (Cd and nickel (Ni from a contaminated soil by washing process. Ethylenediaminetetraacetic acid disodium salt (Na2EDTA and hydrochloric acid (HCl solution were used as extractants. Soil washing is one of the most suitable in-situ/ ex-situ remediation method in removing heavy metals. Soil was artificially contaminated with 500 mg/kg (Pb , Cd and Ni . A set of batch experiments were carried out at different conditions of extractant concentration , contact time, pH and agitation speed. The results showed that the maximum removal efficiencies of (Cd, Pb and Ni were (97, 88 and 24 % respectively using ( 0.1 M Na2EDTA. While the maximum removal efficiencies using (1M HCl were (98, 94 and 55% respectively. The experimental data of batch extraction were applied in four kinetic models; first order, parabolic diffusion, two constant and Elovich model. The parabolic diffusion was the most fitted to the experimental data.

  15. Ethylenediaminetetraacetate and lysozyme improves antimicrobial activities of ovotransferrin against Escherichia coli O157:H7.

    Science.gov (United States)

    Ko, K Y; Mendoncam, A F; Ismail, H; Ahn, D U

    2009-02-01

    The aim of this study was to evaluate the effect of EDTA, lysozyme, or the combination of EDTA and lysozyme on the antibacterial activity of ovotransferrin against Escherichia coli O157:H7. Ovotransferrin solutions (20 mg/mL) containing 100 mM NaHCO3 (OS) with added EDTA (2.0 or 2.5 mg/mL), lysozyme (1.0, 1.5, or 2.0 mg/mL), or both were prepared. The antibacterial activities of OS, OSE (OS+EDTA), or OSL (OS+lysozyme) against E. coli O157:H7 in model systems were investigated by turbidity and viability tests. In addition, OSE, OSL, or OSEL (OS+EDTA+lysozyme) was applied to irradiated pork chops and commercial hams to determine whether the solutions had antibacterial activity on meat products. The effect of the initial cell population on the antibacterial activity of OSE, OSL, and OSEL was determined. Ethylenediaminetetraacetate at 2 mg/mL plus OS induced a reduction of approximately 3 to 4 log in viable E. coli O157:H7 cells in brain heart infusion broth media, and 1 mg/mL of lysozyme plus OS resulted in a reduction of approximately 0.5 to 1.0 log during a 36-h incubation at 35 degrees C. However, neither OSE nor OSEL showed a significant antibacterial effect on pork chops and hams during storage at 10 degrees C. The initial cell number in media did not affect the antibacterial activity of OSE or OSEL against E. coli O157:H7. This study demonstrates that combinations of ovotransferrin, NaHCO3, and EDTA have the potential to control E. coli O157:H7.

  16. Heavy metal phytoextraction-natural and EDTA-assisted remediation of contaminated calcareous soils by sorghum and oat.

    Science.gov (United States)

    Mahmood-Ul-Hassan, Muhammad; Suthar, Vishandas; Ahmad, Rizwan; Yousra, Munazza

    2017-10-30

    The abilities of sorghum (Sorghum bicolor L.) and oat (Avena sativa L.) to take up heavy metals from soils amended with ethylenediaminetetraacetic acid (EDTA) were assessed under greenhouse conditions. Both plants were grown in two soils contaminated with heavy metals (Gujranwala-silty loam and Pacca-clay loam). The soils were treated with 0, 0.625, 1.25, and 2.5 mM EDTA kg -1 soil applied at both 45 and 60 days after sowing (DAS); the experiment was terminated at 75 DAS. Addition of EDTA significantly increased concentrations of Cd, Cr, and Pb in roots and shoots, and bio-concentration factors and phytoextraction rates were also increased. Post-harvest soil analysis showed that soluble fractions of metals were also increased significantly. The increase in Cd was ≈ 3-fold and Pb was ≈ 15-fold at the highest addition of EDTA in Gujranwala soil; in the Pacca soil, the increase was less. Similarly, other phytoremediation factors, such as metal translocation, bio-concentration factor, and phytoextraction, efficiency were also maximum when soils were treated with 2.5 mM EDTA kg -1 soil. The study demonstrated that sorghum was better than oat for phytoremediation.

  17. Effect of EDTA on quartz and hematite flotation with starch/amine in an aqueous solution containing Mn2+ ions

    Directory of Open Access Journals (Sweden)

    Deisiane Ferreira Lelis

    Full Text Available Abstract In this paper, a detailed study of the influence of Mn2+ ions on quartz and hematite flotation (at pH 10.5 with starch/amine as depressor/collector in the absence and presence of ethylenediaminetetraacetic acid (EDTA is presented. By using zeta potential measurement and a careful analysis of manganese species present in water for dosages in which manganese hydroxide precipitated, we confirmed that the depression of both minerals by Mn2+ ions is due to the following: (i the adsorption of Mn hydroxy complexes (Mn2(OH3+ and Mn(OH+; (ii Mn2+ and mainly (iii the precipitation of Mn(OH2 on mineral surfaces. These effects hindered the adsorption of amine species on the surfaces of the minerals (quartz and hematite. EDTA was used to complex Mn2+ to restore the recoveries of both minerals. This was confirmed by the species distribution diagrams of Mn and EDTA-Mn in water. Conditioning with starch followed by amine at pH 10.5 enabled a selective separation of these minerals.

  18. EDTA-assisted Pb phytoextraction.

    Science.gov (United States)

    Saifullah; Meers, E; Qadir, M; de Caritat, P; Tack, F M G; Du Laing, G; Zia, M H

    2009-03-01

    Pb is one of the most widespread and metal pollutants in soil. It is generally concentrated in surface layers with only a minor portion of the total metal found in soil solution. Phytoextraction has been proposed as an inexpensive, sustainable, in situ plant-based technology that makes use of natural hyperaccumulators as well as high biomass producing crops to help rehabilitate soils contaminated with heavy metals without destructive effects on soil properties. The success of phytoextraction is determined by the amount of biomass, concentration of heavy metals in plant, and bioavailable fraction of heavy metals in the rooting medium. In general, metal hyperaccumulators are low biomass, slow growing plant species that are highly metal specific. For some metals such as Pb, there are no hyperaccumulator plant species known to date. Although high biomass-yielding non-hyperaccumulator plants lack an inherent ability to accumulate unusual concentrations of Pb, soil application of chelating agents such as EDTA has been proposed to enhance the metal concentration in above-ground harvestable plant parts through enhancing the metal solubility and translocation from roots to shoots. Leaching of metals due to enhanced mobility during EDTA-assisted phytoextraction has been demonstrated as one of the potential hazards associated with this technology. Due to environmental persistence of EDTA in combination with its strong chelating abilities, the scientific community is moving away from the use of EDTA in phytoextraction and is turning to less aggressive alternative strategies such as the use of organic acids or more degradable APCAs (aminopolycarboxylic acids). We have therefore arrived at a point in phytoremediation research history in which we need to distance ourselves from EDTA as a proposed soil amendment within the context of phytoextraction. However, valuable lessons are to be learned from over a decade of EDTA-assisted phytoremediation research when considering the

  19. Simultaneous determination of oxalic, citric, nitrilotriacetic and ethylenediamenetetraacetic acids by gas liquid chromatography of their methyl esters

    International Nuclear Information System (INIS)

    Eskell, C.J.; Pick, M.E.

    1980-04-01

    A procedure for simultaneous determination of ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA), citric acid and oxalic acid by gas liquid chromatography is described. The involatile acids are first concerted to their volatile methyl ester derivatives by reaction with boron trifluoride in methanol. Transition metal ions (Fe 3+ , Cr 3+ and Ni 2+ ) which will be present in decontamination liquors from nuclear reactors, and form strong chelates with the acids, have been shown to cause no interference to the esterification reaction. The esters were separated by temperature programming on a 3.5 metre capillary column packed with 3% OV1 on Diatomite CQ and were detected by flame ionisation. (author)

  20. Synthesis of selenium/EDTA-derived porous carbon composite as a Li–Se battery cathode

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Chenhao, E-mail: zhaochenhao123456@163.com; Fang, Shuzhen; Hu, Zhibiao, E-mail: zhibiaohu@163.com; Qiu, Sheng’en; Liu, Kaiyu [LongYan University, College of Chemistry & Materials Science (China)

    2016-07-15

    The carbon substrate with unique 3D macroporous structure has been prepared through the immediate carbonization of ethylenediaminetetraacetic acid (EDTA) and KOH mixture. The porous carbon composed of micro- and small mesoporous (2–5 nm) structure has a BET specific surface area of 1824.8 m{sup 2} g{sup −1}. The amorphous and nanosized Se is uniformly encapsulated into the porous structure of porous carbon using melting diffusion route, and the weight content of Se in target Se/C composite can be as high as ~50 %. As an Li–Se battery cathode, the Se/C composite delivers a reversible (2nd) discharge capacity of 597.4 mAh g{sup −1} at 0.24C and retains a discharge capacity of 538.4 mAh g{sup −1} at 0.24C after 100 cycles. Furthermore, the composite also has a stable capacity of 291.0 mAh g{sup −1} at a high current of 4.8C. The high specific area and good porous size of EDTA-derived carbon substrate may a be responsibility for the excellent electrochemical performances of Se/C composite.

  1. Isolate-specific effects of patulin, penicillic Acid and EDTA on biofilm formation and growth of dental unit water line biofilm isolates.

    Science.gov (United States)

    Liaqat, Iram; Bachmann, Robert Thomas; Sabri, Anjum Nasim; Edyvean, Robert G J

    2010-08-01

    Dental unit water line (DUWL) contamination by opportunistic pathogens has its significance in nosocomial infection of patients, health care workers, and life-threatening infections to immunocompromized persons. Recently, the quorum sensing (QS) system of DUWL isolates has been found to affect their biofilm-forming ability, making it an attractive target for antimicrobial therapy. In this study, the effect of two quorum-sensing inhibitory compounds (patulin; PAT, penicillic acid; PA) and EDTA on planktonic growth, AI-2 signalling and in vitro biofilm formation of Pseudomonas aeruginosa, Achromobacter xylosoxidans and Achromobacter sp. was monitored. Vibrio harveyi BB170 bioassay and crystal violet staining methods were used to detect the AI-2 monitoring and biofilm formation in DUWL isolates, respectively. The V. harveyi BB170 bioassay failed to induce bioluminescence in A. xylosoxidans and Achromobacter sp., while P. aeruginosa showed AI-2 like activity suggesting the need of some pretreatments prior to bioassay. All strains were found to form biofilms within 72 h of incubation. The QSIs/EDTA combination have isolate-specific effects on biofilm formation and in some cases it stimulated biofilm formation as often as it was inhibited. However, detailed information about the anti-biofilm effect of these compounds is still lacking.

  2. Synthesis and characterization of TEP-EDTA-regulated bioactive hydroxyapatite

    Science.gov (United States)

    Haders, Daniel Joseph, II

    Hydroxyapatite (HA), Ca10(PO4)6(OH) 2, the stoichiometric equivalent of the ceramic phase of bone, is the preferred material for hard tissue replacement due to its bioactivity. However, bioinert metals are utilized in load-bearing orthopedic applications due to the poor mechanical properties of HA. Consequently, attention has been given to HA coatings for metallic orthopedic implants to take advantage of the bioactivity of HA and the mechanical properties of metals. Commercially, the plasma spray process (PS-HA) is the method most often used to deposit HA films on metallic implants. Since its introduction in the 1980's, however, concerns have been raised about the consequences of PS-HA's low crystallinity, lack of phase purity, lack of film-substrate chemical adhesion, passivation properties, and difficulty in coating complex geometries. Thus, there is a need to develop inexpensive reproducible next-generation HA film deposition techniques, which deposit high crystallinity, phase pure, adhesive, passivating, conformal HA films on clinical metallic substrates. The aim of this dissertation was to intelligently synthesize and characterize the material and biological properties of HA films on metallic substrates synthesized by hydrothermal crystallization, using thermodynamic phase diagrams as the starting point. In three overlapping interdisciplinary studies the potential of using ethylenediamine-tetraacetic acid/triethyl phosphate (EDTA/TEP) doubly regulated hydrothermal crystallization to deposit HA films, the TEP-regulated, time-and-temperature-dependent process by which films were deposited, and the bioactivity of crystallographically engineered films were investigated. Films were crystallized in a 0.232 molal Ca(NO3)2-0.232 molal EDTA-0.187 molal TEP-1.852 molal KOH-H2O chemical system at 200°C. Thermodynamic phase diagrams demonstrated that the chosen conditions were expected to produce Ca-P phase pure HA, which was experimentally confirmed. EDTA regulation of

  3. Modification of electrospun polyacrylonitrile nanofibers with EDTA for the removal of Cd and Cr ions from water effluents

    Energy Technology Data Exchange (ETDEWEB)

    Chaúque, Eutilério F.C., E-mail: efchauque@gmail.com [Department of Applied Chemistry, University of Johannesburg, Doornfontein 2028, Johannesburg (South Africa); Dlamini, Langelihle N., E-mail: lndlamini@uj.ac.za [Department of Applied Chemistry, University of Johannesburg, Doornfontein 2028, Johannesburg (South Africa); Adelodun, Adedeji A., E-mail: aadelodun@uj.ac.za [Department of Applied Chemistry, University of Johannesburg, Doornfontein 2028, Johannesburg (South Africa); Greyling, Corinne J., E-mail: GreylingC@cput.ac.za [Technology Station in Clothing and Textiles, Cape Peninsula University of Technology, Symphony Way, Bellville, 7535 (South Africa); Catherine Ngila, J., E-mail: jcngila2002@yahoo.com [Department of Applied Chemistry, University of Johannesburg, Doornfontein 2028, Johannesburg (South Africa)

    2016-04-30

    Graphical abstract: - Highlights: • Polyscrylonitrile (PAN) nanofibers prepared through electrospinning and chemically modified with ethylenediaminetetraacetic acid using ethylenediamine crosslinker. • Fabricated nanofibers have enhanced surface chemistry with insignificant impact on the nanofibrous structure. • Excellent maximum adsorption capacities of 66.24 and 32.68 mg g{sup −1} toward Cr and Cd ions, respectively. • A pre-concentration factor of 19 achieved for removal of Cd and Cr in environmental water samples. - Abstract: Polyacrylonitrile (PAN) nanofibers were obtained by electrospinning technique prior to surface modification with polyethylenediaminetetraacetic acid (EDTA) using ethylenediamine (EDA) as the cross-linker. The modified nanofibers (EDTA-EDA-PAN) were subsequently applied in the wastewater treatment for the removal of Cd(II) and Cr(VI). Textural and chemical characterizations of the nanofibers were carried out by analysis of the specific surface area (Brauner Emmet and Teller (BET)) and thermogravimetric analyses, scanning electron microscopy and Fourier transform infrared spectroscopy. From the adsorption equilibrium studies with Langmuir, Freundlich and Temkin isotherm models, Freundlich was found most suitable for describing the removal mechanism of the target metals as they collect on a heterogeneously functionalized polymer surface. The EDTA-EDA-PAN nanofibers showed effective sorption affinity for both Cd(II) and Cr(VI), achieving maximum adsorption capacities of 32.68 and 66.24 mg g{sup -1}, respectively, at 298 K. In furtherance, the nanofibers were regenerated by simple washing with 2 M HCl solution. Conclusively, the EDTA-EDA-PAN nanofibers were found to be efficient for the removal of Cd(II) and Cr(VI) in water effluents.

  4. A Validated Reverse Phase HPLC Method for the Determination of Disodium EDTA in Meropenem Drug Substance with UV-Detection using Precolumn Derivatization Technique

    Directory of Open Access Journals (Sweden)

    Bhavil Narola

    2011-01-01

    Full Text Available This paper deals with development and validation of a high performance liquid chromatographic method for the quantitative determination of disodium EDTA (Ethylenediaminetetraacetic acid in Meropenem active pharmaceutical ingredient (API. EDTA was derivatized with Ferric chloride solution by heating at 70 ° C in water bath for about 20 minutes and the chromatographic separation achieved by injecting 100 μL of the derivatized mixture into a Waters HPLC system with photodiode array detector using a Phenomenex Luna C18(2 column (250 × 4.6 mm, 5 μ, The mobile phase consisting of 5% methanol and 95% of 0.7 g/L solution of Tetra butyl ammonium bromide and 4.6 g/L solution of sodium acetate trihydrate in water (pH adjusted to 4.0 with the help of acetic acid glacial and a flow rate of 1 milliliter/minute. EDTA eluted at approximately 6 minutes. The method was suitably validated with respect to specificity, linearity of response, precision, accuracy, ruggedness, stability in analytical solution, limit of quantitation and detection and robustness for its intended use.

  5. Ethylenediaminetetraacetates of neodymium and alkaline earth metals

    International Nuclear Information System (INIS)

    Barinov, A.V.; Pechurova, L.I.; Martynenko, K.I.; Popov, K.I.; Spitsyn, V.I.

    1977-01-01

    The possibilities have been studied of the formation of polynuclear complexonates of alkaline-earth metals (Ca, Sr, and Ba) based on neodymium derivatives EDTA of the composition NdA - . By pH-metry, electron spectroscopy, and derivatography it has been shown that the structure of complexes M 2 (NdA) 2 (where M- Ca, Sr, or Ba; A- EDTA) in the solution is not polynuclear. Hydroxopolynuclear complexes do not form under conditions studied. The data obtained agree with an assumption about polynuclear structure of the solid complex Ca(NdA) 2 x17 H 2 O and gradual weakening of the polynuclear nature upon substitution of Ca 2+ with Sr 2+ and Ba 2+

  6. Ascorbyl palmitate, gamma-tocopherol, and EDTA affect lipid oxidation in fish oil enriched salad dressing differently

    DEFF Research Database (Denmark)

    Let, M.B.; Jacobsen, Charlotte; Meyer, Anne S.

    2007-01-01

    The aim of the study was to investigate the ability of γ-tocopherol, ethylenediaminetetraacetate (EDTA), and ascorbyl palmitate to protect fish oil enriched salad dressing against oxidation during a 6 week storage period at room temperature. The lipid-soluble γ-tocopherol (220 and 880 µg g-1...

  7. Enhanced Fenton-like degradation of TCE in sand suspensions with magnetite by NTA/EDTA at circumneutral pH.

    Science.gov (United States)

    Wang, Na; Jia, Daqing; Jin, Yaoyao; Sun, Sheng-Peng; Ke, Qiang

    2017-07-01

    The present study investigated the degradation of trichloroethylene (TCE) in sand suspensions by Fenton-like reaction with magnetite (Fe 3 O 4 ) in the presence of various chelators at circumneutral pH. The results showed that ethylenediaminetetraacetic acid (EDTA) and nitrilotriacetic acid (NTA) greatly improved the rate of TCE degradation, while [S,S]-ethylenediaminedisuccinic acid (s,s-EDDS), malonate, citrate, and phytic acid (IP6) have minimal effects on TCE degradation. Quenching tests suggested that TCE was mainly degraded by hydroxyl radical (HO · ) attack, with about 90% inhibition on TCE degradation by the addition of HO · scavenger 2-propanol. The presence of 0.1-0.5% Fe 3 O 4 /sand (w/w) contributed to 40% increase in TCE degradation rates. In particular, the use of chelators can avoid high concentrations of H 2 O 2 required for the Fenton-like reaction with Fe 3 O 4 , and moreover improve the stoichiometric efficiencies of TCE degradation to H 2 O 2 consumption. The suitable concentrations of chelators (EDTA and NTA) and H 2 O 2 were suggested to be 0.5 and 20 mM, respectively. Under the given conditions, degradation rate constants of TCE were obtained at 0.360 h -1 with EDTA and 0.526 h -1 with NTA, respectively. Enhanced degradation of TCE and decreased usage of H 2 O 2 in this investigation suggested that Fenton-like reaction of Fe 3 O 4 together with NTA (or EDTA) may be a promising process for remediation of TCE-contaminated groundwater.

  8. The biofilm matrix destabilizers, EDTA and DNaseI, enhance the susceptibility of nontypeable Hemophilus influenzae biofilms to treatment with ampicillin and ciprofloxacin.

    Science.gov (United States)

    Cavaliere, Rosalia; Ball, Jessica L; Turnbull, Lynne; Whitchurch, Cynthia B

    2014-08-01

    Nontypeable Hemophilus influenzae (NTHi) is a Gram-negative bacterial pathogen that causes chronic biofilm infections of the ears and airways. The biofilm matrix provides structural integrity to the biofilm and protects biofilm cells from antibiotic exposure by reducing penetration of antimicrobial compounds into the biofilm. Extracellular DNA (eDNA) has been found to be a major matrix component of biofilms formed by many species of Gram-positive and Gram-negative bacteria, including NTHi. Interestingly, the cation chelator ethylenediaminetetra-acetic acid (EDTA) has been shown to reduce the matrix strength of biofilms of several bacterial species as well as to have bactericidal activity against various pathogens. EDTA exerts its antimicrobial activity by chelating divalent cations necessary for growth and membrane stability and by destabilizing the matrix thus enhancing the detachment of bacterial cells from the biofilm. In this study, we have explored the role of divalent cations in NTHi biofilm development and stability. We have utilized in vitro static and continuous flow models of biofilm development by NTHi to demonstrate that magnesium cations enhance biofilm formation by NTHi. We found that the divalent cation chelator EDTA is effective at both preventing NTHi biofilm formation and at treating established NTHi biofilms. Furthermore, we found that the matrix destablilizers EDTA and DNaseI increase the susceptibility of NTHi biofilms to ampicillin and ciprofloxacin. Our observations indicate that DNaseI and EDTA enhance the efficacy of antibiotic treatment of NTHi biofilms. These observations may lead to new strategies that will improve the treatment options available to patients with chronic NTHi infections. © 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

  9. In-situ synthesis of 3D GA on titanium wire as a binder- free electrode for electro-Fenton removing of EDTA-Ni.

    Science.gov (United States)

    Wen, Shulong; Niu, Zhuyu; Zhang, Zhen; Li, Lianghao; Chen, Yuancai

    2018-01-05

    Ethylenediaminetetraacetic acid (EDTA) could form stable complexes with toxic metals such as nickel due to its strong chelation. The three-dimensional (3D) macroporous graphene aerogels (GA), which was in-situ assembled by reduced graphene oxide (rGO) sheets on titanium wire as binder-free electrode, was presented as cathode for the degradation of EDTA-Ni in Electro-Fenton process. The X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscope (TEM) and Brunauer-Emmett-Teller (BET) results indicated 3D GA formed three dimensional architecture with large and homogenous macropore structure and surface area. Cyclic Voltammetry (CV), Linear Sweep Voltammetry (LSV) and Rotating Ring-disk Electrode (RRDE) results showed that the 3D GA cathode at pH 3 displayed the highest current density and electrochemical active surface area (ECSA), and better two-electron selectivity for ORR than other pH value, confirming the 3D-GA cathode at pH 3 has the highest electrocatalytic activity and generates more H 2 O 2 . The factors such as pH, applied current density, concentration of Fe 2+ , Na 2 SO 4, and aeration rates of air were also investigated. Under the optimum conditions, 73.5% of EDTA-Ni was degraded after reaction for 2h. Mechanism analysis indicated that the production of OH on the 3D GA cathode played an important role in the removal of EDTA-Ni in the 3D GA-EF process, where the direct regeneration of Fe 2+ on the cathode would greatly reduce the consumption of H 2 O 2 . Therefore, it is of great promise for 3D-GA catalyst to be developed as highly efficient, cost-effective and durable cathode for the removal of EDTA-Ni. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. Kinetics of electrophilic substitution of neodymium(3) by ytterbium(3) in aqueous solution of ethylenediaminetetraacetate solution of ethylenediaminetetraacetate and cyclohexanediaminetetraacetate

    Energy Technology Data Exchange (ETDEWEB)

    Nikitenko, S.I.; Martynenko, L.I.; Pechurova, N.I.; Spitsyn, V.I. (Moskovskij Gosudarstvennyj Univ. (USSR))

    1981-01-01

    The process of electrophilic substitution is studied spectrophotometrically taking as an example neodymium (3) substitution for ytterbium (3) in solutions, containing NdL/sup -/ and Yb/sup 3 +/, where L/sup 4 -/ = A/sup 4 -/ and D/sup 4 -/ (ethylenediaminetetraacetate- and cyclohexanediaminetetraacetate). The study of the exchange kinetics is carried out in pH range of 4-6. For the system with L=A in pH range 4.0-4.8 the exchange is realized by the way of acidic dissociation, at pH 5.5-6.0 - by the way of the direct attack. In the system with L=D in pH range 3.5-4.0 the way of acid dissociation prevails, at the same time, the rate constant of acidic dissociation for NdD/sup -/ is two order lower the constant for NdA/sup -/. However, at pH 4.7 the machanism of spontaneous dissociation prevails in the system. Thus, the structure of the complex has considerable effect on the mechanism of electrophilic substitution in rare earth complexes.

  11. Kinetics of electrophilic substitution of neodymium(3) by ytterbium(3) in aqueous solution of ethylenediaminetetraacetate solution of ethylenediaminetetraacetate and cyclohexanediaminetetraacetate

    International Nuclear Information System (INIS)

    Nikitenko, S.I.; Martynenko, L.I.; Pechurova, N.I.; Spitsyn, V.I.

    1981-01-01

    The process of electrophilic substitution is studied spectrophotometrically taking as an example neodymium (3) substitution for ytterbium (3) in solutions, containing NdL - and Yb 3+ , where L 4- = A 4- and D 4- (ethylenediaminetetraacetate- and cyclohexanediaminetetraacetate). The study of the exchange kinetics is carried out in pH range of 4-6. For the system with L=A in pH range 4.0-4.8 the exchange is realized by the way of acidic dissociation, at pH 5.5-6.0 - by the way of the direct attack. In the system with L=D in pH range 3.5-4.0 the way of acid dissociation prevails, at the same time, the rate constant of acidic dissociation for NdD - is two order lower the constant for NdA - . However, at pH 4.7 the machanism of spontaneous dissociation prevails in the system. Thus, the structure of the complex has considerable effect on the mechanism of electrophilic substitution in rare earth complexes [ru

  12. Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations.

    Science.gov (United States)

    Polyakova, Maryna; Schlögl, Haiko; Sacher, Julia; Schmidt-Kassow, Maren; Kaiser, Jochen; Stumvoll, Michael; Kratzsch, Jürgen; Schroeter, Matthias L

    2017-06-03

    Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at -80 °C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations ( n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment ( r = 0.455 to r s = 0.596; p plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations.

  13. Multifunctional adhesive polymers: Preactivated thiolated chitosan-EDTA conjugates.

    Science.gov (United States)

    Netsomboon, Kesinee; Suchaoin, Wongsakorn; Laffleur, Flavia; Prüfert, Felix; Bernkop-Schnürch, Andreas

    2017-02-01

    The aim of this study was to synthesis preactivated thiolated chitosan-EDTA (Ch-EDTA-cys-2MNA) conjugates exhibiting in particular high mucoadhesive, cohesive and chelating properties. Thiol groups were coupled with chitosan by carbodiimide reaction and further preactivated by attachment with 2-mercaptonicotinic acid (2MNA) via disulfide bond formation. Determinations of primary amino and sulfhydryl groups were performed by TNBS and Ellman's tests, respectively. Cytotoxicity was screened by resazurin assay in Caco-2 cells. Mucoadhesive properties and bivalent cation binding capacity with Mg 2+ and Ca 2+ in comparison to chitosan-EDTA (Ch-EDTA) and thiolated Ch-EDTA (Ch-EDTA-cys) were evaluated. Determination of 2MNA and total sulfhydryl groups indicated that 80% of thiol groups were preactivated. The results from cytotoxicity studies demonstrated that Ch-EDTA-cys and Ch-EDTA-cys-2MNA were not toxic to the cells at the polymer test concentration of 0.25% (w/v) while cell viability decreased by increasing the concentration of Ch-EDTA. Although EDTA molecule was modified by thiolation and preactivation, approximately 50% of chelating properties of the conjugates were maintained compared to Ch-EDTA. Ch-EDTA-cys-2MNA adhered on freshly excised porcine intestinal mucosa up to 6h while Ch-EDTA adhered for just 1h. According to the combination of mucoadhesive and chelating properties of the conjugates synthesized in this study, Ch-EDTA-cys-2MNA might be useful for various mucosal drug delivery systems. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Solid-state chelation of metal ions by ethylenediaminetetraacetate intercalated in a layered double hydroxide.

    Science.gov (United States)

    Tarasov, Konstantin A; O'Hare, Dermot; Isupov, Vitaly P

    2003-03-24

    The solid-state chelation of transition metal ions (Co(2+), Ni(2+), and Cu(2+)) from aqueous solutions into the lithium aluminum layered double hydroxide ([LiAl(2)(OH)(6)]Cl x 0.5H(2)O or LDH) which has been pre-intercalated with EDTA (ethylenediaminetetraacetate) ligand has been investigated. The intercalated metal cations form [M(edta)](2)(-) complexes between the LDH layers as indicated by elemental analysis, powder X-ray diffraction, and IR and UV-vis spectroscopies. If metal chloride or nitrate salts are used in the reaction with the LDH then co-intercalation of either the Cl(-) or NO(3)(-) anions is observed. In the case of metal acetate salts the cations intercalate without the accompanying anion. This can be explained by the different intercalation selectivity of the anions in relation to the LDH. In the latter case the introduction of the positive charge into LDH structure was compensated for by the release from the solid of the equivalent quantity of lithium and hydrogen cations. Time-resolved in-situ X-ray diffraction measurements have revealed that the chelation/intercalation reactions proceed very quickly. The rate of the reaction found for nickel acetate depends on concentration as approximately k[Ni(Ac)(2)](3).

  15. Growth of copper-treated corn roots as affected by EDTA, IAA, succinic acid-2,2-dimethyl hydrazide, vitamins and potassium

    Energy Technology Data Exchange (ETDEWEB)

    Hunter, R; Welkie, G W

    1977-04-01

    Corn roots were treated for 1 hr in a modified Hoagland's solution containing 8 mg/l Cu/sup 2 +/ and either simultaneously or subsequently with various substances, in order to test theories of copper toxicity to roots. Post treatment with IAA, niacin amide, thiamin, or sucrose had no effect on subsequent growth. Addition of excess KCl and succinic acid-2,2-dimethyl hydrazide (an inhibitor of ethylene production) to the copper solution doubled average growth and increased recovery from 27 to 87%. A rinse with EDTA resulted in resumption of normal growth rates and 100% recovery if performed 1 hr after Cu treatment, but this effect was gradually lost in the following 6 to 12 hr. Copper toxicity was increasingly severe as pH of the Cu treating medium increased. Results are not consistent with any theory tested, but they do suggest there is an initial phase of copper-stress which lasts 3 to 6 hr, followed by plant reaction that results in irreversible cessation of growth.

  16. The attachment of V79 and human periodontal ligament fibroblasts on periodontally involved root surfaces following treatment with EDTA, citric acid, or tetracycline HCL: an SEM in vitro study.

    Science.gov (United States)

    Chandra, R Viswa; Jagetia, Ganesh Chandra; Bhat, K Mahalinga

    2006-02-15

    The present in vitro study has been designed to establish and compare the effects of citric acid, EDTA, and tetracycline HCl on human periodontally diseased roots on the structure, attachment, and orientation of V79 (primary Chinese hamster lung fibroblasts) cells and human periodontal ligament fibroblasts (HPDL). Commercially available V79 cells and HPDL derived from healthy human third molars were used in this study. These fibroblasts were left in solution for seven days in order to attain confluence. Forty single-rooted teeth were obtained from patients diagnosed with periodontitis. The crown part was removed under constant irrigation and the root was split vertically into two equal halves, thus, yielding 80 specimens. Following scaling and root planing, the specimens were washed with phosphate buffered saline (PBS) and kept in 50 microg/ml gentamycin sulphate solution for 24 hours. The root pieces were then treated as follows: citric acid at pH 1, 24% EDTA, or with a 10% solution of tetracycline HCl and were then placed in V79 fibroblast cultures and HPDL cultures. The specimens were harvested after four weeks and were fixed in 2.5% glutaraldehyde in PBS before preparation for scanning electron microscopy (SEM). The behavior of V79 cells was similar to that of human periodontal ligament cells on root conditioned surfaces. V79 and HPDL showed a healthy morphology on root surfaces treated with citric acid and EDTA and a relatively unhealthy appearance on root surfaces treated with tetracycline HCl and distilled water (control group). The results suggest the use of citric acid and EDTA as root conditioning agents favorably affects the migration, attachment, and morphology of fibroblasts on human root surfaces, which may play a significant role in periodontal healing and regeneration.

  17. Synthesis of tungsten oxide (W{sub 18}O{sub 49}) nanosheets utilizing EDTA salt by microwave irradiation method

    Energy Technology Data Exchange (ETDEWEB)

    Hariharan, V.; Parthibavarman, M. [Centre for Nanoscience and Technology, Department of Physics, Periyar University, Salem 636 011, Tamilnadu (India); Sekar, C., E-mail: Sekar2025@gmail.com [Centre for Nanoscience and Technology, Department of Physics, Periyar University, Salem 636 011, Tamilnadu (India); Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi 630 003, Tamilnadu (India)

    2011-04-07

    Research highlights: > We have synthesized tungsten oxide (WO{sub 3-{delta}}) nanoparticles by microwave irradiation method for the first time using EDTA as surface modulator. The variation in stoichiometric oxygen content of the annealed samples clearly indicates the role of EDTA in reaction medium. The variation in oxygen content also modified the transparency of the end product confirming the change in optical conductivity. - Abstract: We report the synthesis of crystalline W{sub 18}O{sub 49} with nanosheet like morphology by low cost microwave irradiation method without employing hydrothermal process for the first time. Initially, WO{sub 3}.H{sub 2}O was synthesized using ethylenediaminetetraacetic acid (EDTA) as surface modulator. The product was annealed at 600 {sup o}C for 6 h in ambient atmosphere in order to obtain anhydrous tungsten oxide W{sub 18}O{sub 49}. Powder X-ray diffraction results confirmed the as prepared WO{sub 3}.H{sub 2}O to be orthorhombic and W{sub 18}O{sub 49} to be monoclinic phase, respectively. Transmission electron micrographs (TEM) revealed that the W{sub 18}O{sub 49} nanosheets have the average dimensions of the order of 250 nm in length and around 150 nm in width. UV-visible diffusion reflectance spectroscopic (DRS) studies revealed the band gap energies to be 3.28 and 3.47 eV for WO{sub 3}.H{sub 2}O and W{sub 18}O{sub 49} samples, respectively. The growth mechanism of two dimensional W{sub 18}O{sub 49} nanosheets is discussed.

  18. Ligand-enhanced electrokinetic remediation of metal-contaminated marine sediments with high acid buffering capacity.

    Science.gov (United States)

    Masi, Matteo; Iannelli, Renato; Losito, Gabriella

    2016-06-01

    The suitability of electrokinetic remediation for removing heavy metals from dredged marine sediments with high acid buffering capacity was investigated. Laboratory-scale electrokinetic remediation experiments were carried out by applying two different voltage gradients to the sediment (0.5 and 0.8 V/cm) while circulating water or two different chelating agents at the electrode compartments. Tap water, 0.1 M citric acid and 0.1 M ethylenediaminetetraacetic acid (EDTA) solutions were used respectively. The investigated metals were Zn, Pb, V, Ni and Cu. In the unenhanced experiment, the acid front could not propagate due to the high acid buffering capacity of the sediments; the production of OH(-) ions at the cathode resulted in a high-pH environment causing the precipitation of CaCO3 and metal hydroxides. The use of citric acid prevented the formation of precipitates, but solubilisation and mobilisation of metal species were not sufficiently achieved. Metal removal was relevant when EDTA was used as the conditioning agent, and the electric potential was raised up to 0.8 V/cm. EDTA led to the formation of negatively charged complexes with metals which migrated towards the anode compartment by electromigration. This result shows that metal removal from sediments with high acid buffering capacity may be achieved by enhancing the electrokinetic process by EDTA addition when the acidification of the medium is not economically and/or environmentally sustainable.

  19. EDTA-functionalized clinoptilolite nanoparticles as an effective adsorbent for Pb(II) removal.

    Science.gov (United States)

    Eshraghi, Farahnaz; Nezamzadeh-Ejhieh, Alireza

    2018-03-08

    An efficient Pb(II) adsorbent was prepared by the modification of clinoptilolite nanoparticles (CpN) with ethylenediaminetetraacetic acid (EDTA). Samples were characterized by XRD, FT-IR, SEM, BET, TG-DTG, CHNS analyzer, and energy dispersive analysis X-ray spectroscopy (EDX). The experiments were designed by response surface methodology (RSM) based on central composite design (CCD) that suggested a quadratic model to predict the conditions and the interactions between the variables including adsorbent dosage, removal time, C Pb , and its solution pH. Adequacy of the suggested quadratic model was judged by ANOVA. The maximum Pb(II) removal of 0.27 mmol Pb(II) /g ads was achieved in optimal run including adsorbent dosage 2 g L -1 , removal time 271 min, C Pb 22.51 mmol L -1 , and Pb(II) solution pH 5.88. In binary metal cation systems including 1000 mg L -1 with respect to both Pb(II) and interfering cations, good selectivity of CpN-EDTA adsorbent was observed towards Pb(II) among the tested cations except Fe(III). Adsorption isotherm of lead removal by the adsorbent was well modeled by Langmuir equation, indicating a monolayer sorption of Pb(II) onto the adsorbent. The pseudo-second-order rate equation, indicating chemical reaction rate limiting step for the process, well modeled the kinetic of the process. An exothermic and spontaneous process was confirmed by the negative ∆H and ∆G.

  20. Heavy metals adsorption by novel EDTA-modified chitosan-silica hybrid materials.

    Science.gov (United States)

    Repo, Eveliina; Warchoł, Jolanta K; Bhatnagar, Amit; Sillanpää, Mika

    2011-06-01

    Novel adsorbents were synthesized by functionalizing chitosan-silica hybrid materials with (ethylenediaminetetraacetic acid) EDTA ligands. The synthesized adsorbents were found to combine the advantages of both silica gel (high surface area, porosity, rigid structure) and chitosan (surface functionality). The Adsorption potential of hybrid materials was investigated using Co(II), Ni(II), Cd(II), and Pb(II) as target metals by varying experimental conditions such as pH, contact time, and initial metal concentration. The kinetic results revealed that the pore diffusion process played a key role in adsorption kinetics, which might be attributed to the porous structure of synthesized adsorbents. The obtained maximum adsorption capacities of the hybrid materials for the metal ions ranged from 0.25 to 0.63 mmol/g under the studied experimental conditions. The adsorbent with the highest chitosan content showed the best adsorption efficiency. Bi-Langmuir and Sips isotherm model fitting to experimental data suggested the surface heterogeneity of the prepared adsorbents. In multimetal solutions, the hybrid adsorbents showed the highest affinity toward Pb(II). Copyright © 2011 Elsevier Inc. All rights reserved.

  1. Novel EDTA-ligands containing an integral perylene bisimide (PBI) core as an optical reporter unit.

    Science.gov (United States)

    Marcia, Mario; Singh, Prabhpreet; Hauke, Frank; Maggini, Michele; Hirsch, Andreas

    2014-09-28

    The synthesis, characterization and metal complexation of a new class of perylene bisimides (PBIs) as an integral part of ethylenediaminetetraacetic acid (EDTA) are reported. The simplest representative, namely derivative 1a, was synthesized both by a convergent as well as a direct approach while the elongated derivatives, 1b and 1c, were obtained only via a convergent synthetic pathway. All these new prototypes of water-soluble perylenes are bolaamphiphiles and were fully characterized by (1)H- and (13)C-NMR spectroscopy, matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry and IR spectroscopy. In order to acquaint ourselves with the behaviour in solution of our PBIs bearing dendritic wedges, the simplest derivative, 1a, was chosen and tested by means of UV/Vis and fluorescence spectroscopy as well as by zeta-potential measurements. A photoexcitation induced intramolecular photo-electron transfer (PET) can be observed in these molecules. Therefore their potential applications as sensors can be imagined. Model compound 1a efficiently coordinates trivalent metal cations both in water and dimethyl sulfoxide (DMSO). Significantly, the effects of the complexation strongly depend on the aggregation state of the PBI molecules in solution. As a matter of fact, in water, the presence of M(3+) ions triggers the formation of light emitting supramolecular aggregates (excimers). On the other hand, in DMSO-rich solutions metal complexation leads to the suppression of the PET and leads to a strong fluorescence enhancement.

  2. Synthesis and photocatalytic application of TiO{sub 2} nanoparticles immobilized on polyacrylonitrile nanofibers using EDTA chelating agents

    Energy Technology Data Exchange (ETDEWEB)

    Chaúque, Eutilério F.C., E-mail: efchauque@gmail.com [Department of Applied Chemistry, University of Johannesburg, Doornfontein, 2028, Johannesburg (South Africa); Adelodun, Adedeji A., E-mail: aadelodun@uj.ac.za [Department of Applied Chemistry, University of Johannesburg, Doornfontein, 2028, Johannesburg (South Africa); Dlamini, Langelihle N., E-mail: lndlamini@uj.ac.za [Department of Applied Chemistry, University of Johannesburg, Doornfontein, 2028, Johannesburg (South Africa); Greyling, Corinne J., E-mail: GreylingC@cput.ac.za [Technology Station in Clothing and Textiles, Cape Peninsula University of Technology, Symphony Way, Belville, 7535 (South Africa); Ray, Sekhar C., E-mail: raysc@unisa.ac.za [Department of Physics, University of South Africa, Florida, 1710, Johannesburg (South Africa); Ngila, J. Catherine, E-mail: jcngila@uj.ac.za [Department of Applied Chemistry, University of Johannesburg, Doornfontein, 2028, Johannesburg (South Africa)

    2017-05-01

    The photocatalytic properties of TiO{sub 2} nanoparticles (TNPs) have been widely demonstrated in the literature. Here, we report the chemical attachment of TNPs to the surface of polyacrylonitrile nanofibers (PNFs) using the ethylenediaminetetraacetic acid (EDTA) and ethylenediamine (EDA) as the chelating agents. The composite nanofibers were prepared through the chelation of Ti{sup 4+} ions with surface carboxylic and amine groups followed by self-growth of TiO{sub 2} nanoparticles on the surface of modified PNFs during the incubation process. The fabricated composite nanofibers were stabilized at 240 °C in a tube furnace under N{sub 2} gas. The heat treatment served to simultaneously crystallize the TNPs and enhance robustness of PNFs as cyclization reactions and the cross-linking of adjacent nitrile groups (–C=N−C=N–) usually takes place at temperatures above 200 °C. Characterization techniques included X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared (FT-IR), Raman spectroscopy and Brunauer-Emmett-Teller (BET) technique. The chemical impregnation of EDTA-EDA on the surface of PNFs resulted in slight increase in the average nanofiber's diameter. The anatase TiO{sub 2} nanoparticles with average 9.4 nm particle size prepared in situ were immobilized on the surface of pre-functionalized PNFs. The fabricated composite nanofibers were applied in the photocatalytic degradation of methyl orange (MO) in synthetic aqueous solutions. The as-prepared composite nanofibers were reused for five (5) cycles without considerable decline in the MO removal efficiency (i.e. >98% of initial performance). - Highlights: • Electrospun polyacrylonitrile nanofibers (PNFs) were chemically modified with EDTA. • The TiO{sub 2}-EDTA-EDA-PNFs composites were prepared through chelation of Ti{sup 4+} ions. • Composites were calcined for simultaneous crystallization and chemical stability. • Ti

  3. Crown ether derivatives of EDTA: Pt. 6

    International Nuclear Information System (INIS)

    Liu Zhongqun; Qin Shengying; Chen Shaojin; Tan Lin

    1988-01-01

    EDTA-diaminodibenzo-18-crown-6 (cis- and trans-) condensation polymer is a new compound of crown ether derivatives of EDTA. In this paper the adsorption behaviors of U(IV) and U(VI) on this polymer from chloride solutions and effects of hydrochloric acid concentrations, salting-out agents and organic solvents on distribution coefficient (K d ) of uranium are investigated. Adsorption mechanism of uranyl ion (UO 2 2+ ) on this polymer was studied with IR spectra and by means of the adsorption behaviors of compounds of similar structure. Experimental results show that both polyether section and carboxyl groups in EDTA-diaminodibenzo-18-crown-6 take part in complexation with uranyl ion and synergistic effect appeared

  4. Simultaneous Speciation, Structure, and Equilibrium Constant Determination in the Ni2+-EDTA-CN- Ternary System via High-Resolution Laboratory X-ray Absorption Fine Structure Spectroscopy and Theoretical Calculations.

    Science.gov (United States)

    Bajnóczi, Éva G; Németh, Zoltán; Vankó, György

    2017-11-20

    Even quite simple chemical systems can involve many components and chemical states, and sometimes it can be very difficult to differentiate them by their hardly separable physical-chemical properties. The Ni II -EDTA-CN - (EDTA = ethylenediaminetetraacetic acid) ternary system is a good example for this problem where, in spite of its fairly simple components and numerous investigations, several molecular combinations can exist, all of them not having been identified unambiguously yet. In order to achieve a detailed understanding of the reaction steps and chemical equilibria, methods are required in which the structural transitions in the different reaction steps can be followed via element-selective complex spectral feature sets. With the help of our recently developed von Hámos type high-resolution laboratory X-ray absorption spectrometer, both the structural variations and stability constants of the forming complexes were determined from the same measurement series, proving that X-ray absorption spectroscopy can be considered as a multifaced, table-top tool in coordination chemistry. Furthermore, with the help of theoretical calculations, independent structural evidence was also given for the formation of the [NiEDTA(CN)] 3- mixed complex.

  5. Synthesis and Characterization of Ethylene DiamineTetera Acetic Acid Polyester-amides polymer with Aminoalcohol

    Directory of Open Access Journals (Sweden)

    Dakhil Nasser Taha

    2017-02-01

    Full Text Available linear aromatic and aliphatic polyester-amides (PEAs have been synthesized by polycondensation of aliphatic and aromatic aminoalcohol (Ethanol amine, 2-amino-2-methyl-propan-1-ol, m-amino phenol with Ethylenediaminetetraacetic acid (EDTA as a favorable and combined complexing compound was joined into the polymer backbone with poly addition reactions. These polymers were characterized by FTIR, 1H NMR, solubility studies , elemental analysis, , Thermal analyses such as TGA were measured, intrinsic viscosity. The poly(ester-amides obtained show good thermal stability.

  6. Investigation of hyperfine interactions in hafnium compounds with F1-, OH1-, and EDTA ligands by perturbed γ-γ angular correlation

    International Nuclear Information System (INIS)

    Amaral, Antonio A.; Silva, Andreia dos S.; Lapolli, Andre L.; Carbonari, Artur W.; Saxena, Rajendra N.

    2011-01-01

    In this study the hyperfine parameters, including the dynamical nature, Perturbed Angular Correlation (PAC) spectroscopy was used to measure the hyperfine parameters in molecules of ligand compounds in solutions. The measurements were carried out at 295 K and 77 K using 181 Hf → 181 Ta, as probe nuclei. Samples were prepared by adding a small volume of radioactive solution containing the probe nuclei in aqueous solution, buffer solution and ethylenediaminetetraacetic acid (EDTA) used as a ligand with pH between 4 and 5 which correspond to the pH of the saturated EDTA solution and in buffer solution with pH between 9 and 10. The results made possible to understand the impact of each method for PAC measurements. Finally a comparative analysis for the several methods of inserting of the probe nuclei in the sample was made, considering chemical and nuclear aspects. The lack of measurements in this kind of samples justifies the importance of the obtained results. (author)

  7. Application of the perturbed angular correlation in the investigation of hyperfine interactions in compounds of hafnium, indium and cadmium with F1-, OH1- and EDTA ligands

    International Nuclear Information System (INIS)

    Amaral, Antonio Acleto

    2011-01-01

    In this study the hyperfine parameters, including the dynamical nature, Perturbed Angular Correlation (PAC) spectroscopy was used to measure the hyperfine parameters in molecules of ligand compounds in solutions. The measurements were carried out at 295 K and 77 K using 111 In → 111 Cd, 181 Hf → 181 Ta and 111m Cd → 111 Cd, as probe nuclei. Samples were prepared by adding a small volume of radioactive solution containing the probe nuclei in aqueous solution, buffer solution and ethylenediaminetetraacetic acid (EDTA) used as a ligand with pH 4.3 which correspond to the pH of the saturated EDTA solution and in buffer solution with pH between 9 and 10. The results made possible to understand the impact of each method for PAC measurements. Finally a comparative analysis for the several methods of inserting of the probe nuclei in the sample was made, considering chemical and nuclear aspects. The lack of measurements in this kind of samples justifies the importance of the obtained results. (author)

  8. Applying carbon dioxide, plant growth-promoting rhizobacterium and EDTA can enhance the phytoremediation efficiency of ryegrass in a soil polluted with zinc, arsenic, cadmium and lead.

    Science.gov (United States)

    Guo, Junkang; Feng, Renwei; Ding, Yongzhen; Wang, Ruigang

    2014-08-01

    This study was conducted to investigate the use of elevated carbon dioxide (CO2), plant growth-promoting rhizobacterium Burkholderia sp. D54 (PGPR) and ethylenediaminetetraacetic acid (EDTA) to enhance the phytoextraction efficiency of ryegrass in response to multiple heavy metal (or metalloid)-polluted soil containing zinc (Zn), arsenic (As), cadmium (Cd) and lead (Pb). All of the single or combined CO2, PGPR and EDTA treatments promoted ryegrass growth. The stimulation of ryegrass growth by CO2 and PGPR could primarily be attributed to the regulation of photosynthesis rather than decreased levels of Zn, As and Cd in the shoots. Most treatments seemed to reduce the Zn, As and Cd contents in the shoots, which might be associated with enhanced shoot biomass, thus causing a "dilution effect" regarding their levels. The combined treatments seemed to perform better than single treatments in removing Zn, As, Cd and Pb from soil, judging from the larger biomass and relatively higher total amounts (TAs) of Zn, As, Cd and Pb in both the shoots and roots. Therefore, we suggest that the CO2 plus PGPR treatment will be suitable for removing Zn, As, Cd and Pb from heavy metal (or metalloid)-polluted soils using ryegrass as a phytoremediation material. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Investigation of hyperfine interactions in hafnium compounds with F{sup 1-}, OH{sup 1-}, and EDTA ligands by perturbed {gamma}-{gamma} angular correlation

    Energy Technology Data Exchange (ETDEWEB)

    Amaral, Antonio A.; Silva, Andreia dos S.; Lapolli, Andre L.; Carbonari, Artur W.; Saxena, Rajendra N., E-mail: alapolli@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    In this study the hyperfine parameters, including the dynamical nature, Perturbed Angular Correlation (PAC) spectroscopy was used to measure the hyperfine parameters in molecules of ligand compounds in solutions. The measurements were carried out at 295 K and 77 K using {sup 181}Hf {yields}{sup 181}Ta, as probe nuclei. Samples were prepared by adding a small volume of radioactive solution containing the probe nuclei in aqueous solution, buffer solution and ethylenediaminetetraacetic acid (EDTA) used as a ligand with pH between 4 and 5 which correspond to the pH of the saturated EDTA solution and in buffer solution with pH between 9 and 10. The results made possible to understand the impact of each method for PAC measurements. Finally a comparative analysis for the several methods of inserting of the probe nuclei in the sample was made, considering chemical and nuclear aspects. The lack of measurements in this kind of samples justifies the importance of the obtained results. (author)

  10. EDTA modified glassy carbon electrode: Preparation and characterization

    International Nuclear Information System (INIS)

    Ustuendag, Zafer; Solak, Ali Osman

    2009-01-01

    EDTA-phenoxyamide modified glassy carbon electrode (EDTA-GC) was prepared at a glassy carbon electrode by surface synthesis. In the first step, nitrophenyl was grafted to the glassy carbon (GC) surface via the electrochemical reduction of its tetraflouroborate diazonium salt. In the second step, nitrophenyl-modified electrode (NP-GC) was subjected to the cathodic potential scan to reduce the nitro to amine group. p-Aminophenyl modified glassy carbon electrode (AP-GC) was dipped into a EDTA solution containing 1-ethyl-3(3-(dimethlyamino)propyl)-carbodiimide (EDC) as an activating agent. Thus formed ((2-anilino-2-oxoethyl){2-[bis(carboxymethyl)amino]-ethyl}amino)acetic acid modified GC electrode was denoted as EDTA-GC and characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), ellipsometry and X-ray photoelectron spectroscopy (XPS). Complexation of the EDTA-GC surface with Pb 2+ ions was investigated if this electrode could be used as a metal sensor.

  11. Coulometric titration of niobium in EDTA complexing solution

    International Nuclear Information System (INIS)

    Pannu, S.S.

    1976-01-01

    Constant current coulometry of Nb in EDTA was studied employing both potentiometric and amperometric end-point techniques. The titrations were based on the oxidation of Nb(IV)-EDTA with electrogenerated Fe(III) at a graphite anode. The rate of disproportionation of Nb(IV)-EDTA was found to be slow enough not to effect the accuracy sought for the titration. The coulometric titration of Nb in EDTA was found to be more accurate than in 1F sulfuric acid. The mean error in the titrations of 4.76 to 19.65 of Nb in a volume of about 100 ml of 0.02 to 0.04F EDTA was 0.13%. It was found that Ta may be tolerated to a ratio of 1:4, Ta:Nb, in a solution containing 13 mg of Nb. (author)

  12. Growth and characterization of EDTA assisted CBD-CdS

    Energy Technology Data Exchange (ETDEWEB)

    Kumarage, W.G.C.; Wijesundera, R. P.; Seneviratne, V. A.; Jayalath, C. P.; Varga, Tamas; Nandasiri, Manjula I.; Dassanayake, B. S.

    2017-10-01

    Chemical bath deposition of CdS (CBD-CdS) thin films with the assistance of a cationic surfactant, ethylenediamine tetraacetic acid (EDTA), is reported in this work. Also the EDTA treated CdS thin films are compared with that of conventional CBD-CdS. Fabricated thin, compact, uniform and adherent EDTA treated CdS films show enhanced effective surface area and roughness compared to conventional CBD-CdS. The grazing incidence x-ray diffraction analysis shows all the fabricated CdS films are hexagonally crystallized. EDTA-treated CdS films show excellent photo activity compared to conventional CBDCdS. The flat band potential (Vfb) value was found to be tunable with EDTA concentration.

  13. Effects of sodium hypochlorite associated with EDTA and etidronate on apical root transportation.

    Science.gov (United States)

    Silva e Souza, P A R; das Dores, R S E; Tartari, T; Pinheiro, T P S; Tuji, F M; Silva e Souza, M H

    2014-01-01

    To evaluate the influence of sodium hypochlorite associated with EDTA and etidronate on apical root transportation. Forty-five roots of human mandibular molars with curvatures of 15-25° were embedded in acrylic resin to allow standardized angulation of the initial and final radiographs. The pre-instrumentation radiographs of the mesiobuccal canal of each root were taken using a radiograph digital sensor with a size 15 K-file in the canal. The canals were prepared with the ProTaper Universal system (Dentsply Maillefer, Ballaigues, Switzerland), using one of the following irrigation regimens during the instrumentation (n = 15): G1 - irrigation with 20 mL of saline solution (control); G2 - alternating irrigation with 2.5% hypochlorite solution (NaOCl) (15 mL); and 17% ethylenediaminetetraacetic acid (EDTA) (5 mL). During instrumentation, the canal was filled with NaOCl and then between each exchange of instrument filled with EDTA for 1 min, and G3 - irrigation with 20 mL of 5% NaOCl and 18% etidronate solution (HEBP) mixed in equal parts. The postinstrumentation radiographs were made with a F3 instrument in the canal. The images were magnified and superposed with Adobe Photoshop software (Adobe Systems, Mountain View, CA, USA). Apical transportation was determined with AutoCAD 2012 software (Autodesk Inc., San Rafael, CA, USA) by measuring the distance in millimetres between the tips of the instruments. The results were subjected to the nonparametric statistical Kruskal-Wallis test (α < 0.05). The median transportation and interquartile range values were 0.00 ± 0.05 for G1, 0.08 ± 0.23 for G2 and 0.13 ± 0.14 for G3. Comparison between groups showed that apical transportation in G3 was significantly greater than in G1 (P < 0.05). The use of NaOCl associated with etidronate increased apical transportation in the canals of extracted teeth. © 2013 International Endodontic Journal. Published by John Wiley & Sons Ltd.

  14. Kinetics of dissolution of {alpha}-Fe{sub 2}O{sub 3} and {gamma}-Fe{sub 2}O{sub 3} in EDTA and NTA-based formulations

    Energy Technology Data Exchange (ETDEWEB)

    Ranganathan, S. [Dept. of Chemical Engineering, Univ. of New Brunswick, Fredericton, N.B. (Canada); Srinivasan, M.P. [Water and Steam Chemistry Lab. of Bhabha Atomic Research Centre (BARC) (India); Narasimhan, S.V. [Bhabha Atomic Research Centre (India); Raghavan, P.S. [Madras Christian Coll., Chennai (India); Gopalan, R. [Dept. of Chemistry, Madras Christian Coll., Chennai (India)

    2004-06-01

    The dissolution studies were carried out on haematite ({alpha}-Fe{sub 2}O{sub 3}) and maghemite ({gamma}-Fe{sub 2}O{sub 3}) in two different formulations of ethylenediaminetetraacetic acid (EDTA) and nitrilotriaceticacid (NTA). The rate constants were calculated using the ''inverse cubic rate law.'' The leaching of the metal ions from the oxide is controlled partly by the Fe(II)-L{sub n} (L is a complexing ligand and n is the number of ligands attached to Fe{sup 2+}), a dissolution product arising from the oxides having Fe{sup 2+} in the lattice. The addition of Fe(II)-L{sub n} along with the formulation greatly increased the initial rate of dissolution. The effect of the addition of Fe(II)-L as a reductant on the dissolution of {alpha}-Fe{sub 2}O{sub 3} was not the same as in the case of {gamma}-Fe{sub 2}O{sub 3}. The rate constants (k{sub obs}) for the dissolution of {alpha}-Fe{sub 2}O{sub 3} and {gamma}-Fe{sub 2}O{sub 3} in the presence of ascorbic acid were less in the EDTA formulation than in the NTA formulation. The studies using Fe(II)-NTA and Fe(II)-EDTA with varying compositions of citric acid and ascorbic acid revealed that a minimum quantity of the chelant is sufficient to initiate the dissolution process, which can be further controlled by the reductants and weaker chelants such as citric acid. (orig.)

  15. Application of the perturbed angular correlation in the investigation of hyperfine interactions in compounds of hafnium, indium and cadmium with F{sup 1-}, OH{sup 1-} and EDTA ligands; Aplicacao da espectroscopia de correlacao angular perturbada na investigacao de interacoes hiperfinas em compostos de hafnio, indio e cadmio com os ligantes F{sup 1-}, OH{sup 1-} e EDTA

    Energy Technology Data Exchange (ETDEWEB)

    Amaral, Antonio Acleto

    2011-07-01

    In this study the hyperfine parameters, including the dynamical nature, Perturbed Angular Correlation (PAC) spectroscopy was used to measure the hyperfine parameters in molecules of ligand compounds in solutions. The measurements were carried out at 295 K and 77 K using {sup 111}In {yields} {sup 111}Cd, {sup 181}Hf {yields} {sup 181}Ta and {sup 111m}Cd {yields}{sup 111}Cd, as probe nuclei. Samples were prepared by adding a small volume of radioactive solution containing the probe nuclei in aqueous solution, buffer solution and ethylenediaminetetraacetic acid (EDTA) used as a ligand with pH 4.3 which correspond to the pH of the saturated EDTA solution and in buffer solution with pH between 9 and 10. The results made possible to understand the impact of each method for PAC measurements. Finally a comparative analysis for the several methods of inserting of the probe nuclei in the sample was made, considering chemical and nuclear aspects. The lack of measurements in this kind of samples justifies the importance of the obtained results. (author)

  16. Acute intestinal injury induced by acetic acid and casein: prevention by intraluminal misoprostol

    International Nuclear Information System (INIS)

    Miller, M.J.; Zhang, x.J.; Gu, x.A.; Clark, D.A.

    1991-01-01

    Acute injury was established in anesthetized rabbits by intraluminal administration of acetic acid with and without bovine casein, into loops of distal small intestine. Damage was quantified after 45 minutes by the blood-to-lumen movement of 51 Cr-labeled ethylenediaminetetraacetic acid (EDTA) and fluorescein isothiocyanate-tagged bovine serum albumin as well as luminal fluid histamine levels. The amount of titratable acetic acid used to lower the pH of the treatment solutions to pH 4.0 was increased by the addition of calcium gluconate. Luminal acetic acid caused a 19-fold increase in 51 Cr-EDTA accumulation over saline controls; casein did not modify this effect. In saline controls, loop fluid histamine levels bordered on the limits of detection (1 ng/g) but were elevated 19-fold by acetic acid exposure and markedly increased (118-fold) by the combination of acid and casein. Intraluminal misoprostol (3 or 30 micrograms/mL), administered 30 minutes before acetic acid, significantly attenuated the increase in epithelial permeability (luminal 51 Cr-EDTA, fluorescein isothiocyanate-bovine serum albumin accumulation) and histamine release (P less than 0.05). Diphenhydramine, alone or in combination with cimetidine, and indomethacin (5 mg/kg IV) were not protective. It is concluded that exposure of the epithelium to acetic acid promotes the transepithelial movement of casein leading to enhanced mast cell activation and mucosal injury. Damage to the epithelial barrier can be prevented by misoprostol

  17. Acute intestinal injury induced by acetic acid and casein: prevention by intraluminal misoprostol

    Energy Technology Data Exchange (ETDEWEB)

    Miller, M.J.; Zhang, x.J.; Gu, x.A.; Clark, D.A. (Department of Pediatrics, Louisiana State University School of Medicine, New Orleans (USA))

    1991-07-01

    Acute injury was established in anesthetized rabbits by intraluminal administration of acetic acid with and without bovine casein, into loops of distal small intestine. Damage was quantified after 45 minutes by the blood-to-lumen movement of {sup 51}Cr-labeled ethylenediaminetetraacetic acid (EDTA) and fluorescein isothiocyanate-tagged bovine serum albumin as well as luminal fluid histamine levels. The amount of titratable acetic acid used to lower the pH of the treatment solutions to pH 4.0 was increased by the addition of calcium gluconate. Luminal acetic acid caused a 19-fold increase in {sup 51}Cr-EDTA accumulation over saline controls; casein did not modify this effect. In saline controls, loop fluid histamine levels bordered on the limits of detection (1 ng/g) but were elevated 19-fold by acetic acid exposure and markedly increased (118-fold) by the combination of acid and casein. Intraluminal misoprostol (3 or 30 micrograms/mL), administered 30 minutes before acetic acid, significantly attenuated the increase in epithelial permeability (luminal {sup 51}Cr-EDTA, fluorescein isothiocyanate-bovine serum albumin accumulation) and histamine release (P less than 0.05). Diphenhydramine, alone or in combination with cimetidine, and indomethacin (5 mg/kg IV) were not protective. It is concluded that exposure of the epithelium to acetic acid promotes the transepithelial movement of casein leading to enhanced mast cell activation and mucosal injury. Damage to the epithelial barrier can be prevented by misoprostol.

  18. Ethyl lactate-EDTA composite system enhances the remediation of the cadmium-contaminated soil by Autochthonous Willow (Salix x aureo-pendula CL 'J1011') in the lower reaches of the Yangtze River

    International Nuclear Information System (INIS)

    Li Jiahua; Sun Yuanyuan; Yin Ying; Ji Rong; Wu Jichun; Wang Xiaorong; Guo Hongyan

    2010-01-01

    In order to explore a practical approach to the remediation of the cadmium (Cd)-contaminated soil in the lower reaches of the Yangtze River, we evaluated the effects of a local willow (Salix x aureo-pendula CL 'J1011') of absorbing, accumulating, and translocating Cd; and assessed the potential of chelator ethylenediaminetetraacetic acid (EDTA) in combination with ethyl lactate for enhancing the efficiency of the willow in removing Cd in two water-culture growth chamber trials and a field one. The willow showed a high tolerance to Cd in growth chamber trial 1 where the Cd concentration in the medium reached up to 25 mg L -1 medium, and the bioaccumulation factors (BAFs) of the shoots for Cd rose from 3.8 to 7.4 as the Cd concentration in the medium was elevated from 5 to 25 mg L -1 medium. In growth chamber trial 2, the average Cd removal rates in two treatments with EDTA and ethyl lactate (molar ratios of EDTA to ethyl lactate = 68/39 and 53.5/53.5, respectively) reached 0.71 mg d -1 pot -1 for the duration of Day 5-8 and 0.59 mg d -1 pot -1 for that of Day 8-11, which were 5- and 4-fold of their counterparts in the control, respectively. In the field trial, for the remediational duration of 45 days, three treatments-willow alone, willow combined with EDTA, and willow combined with EDTA and ethyl lactate-led to decreases in the Cd concentration in soil by 5%, 20%, and 29%, respectively; increases in that in the leaves by 14.6%, 56.7%, and 146.5%, respectively; and increases in that in the stems by 15.6%, 41.2%, and 87.4%, respectively, compared to their counterparts on Day 0. These results indicate that EDTA combined with ethyl lactate significantly enhanced the efficiency of willow in removing Cd from the soil. Therefore, a phytoextration system consisting of the autochthonous willow, EDTA, and ethyl lactate has high potential for the remediation of the Cd-polluted soil in the lower reaches of the Yangtze River.

  19. Ethyl lactate-EDTA composite system enhances the remediation of the cadmium-contaminated soil by Autochthonous Willow (Salix x aureo-pendula CL 'J1011') in the lower reaches of the Yangtze River

    Energy Technology Data Exchange (ETDEWEB)

    Li Jiahua [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Sun Yuanyuan [State Key Laboratory of Pollution Control and Resource Reuse, Department of Hydrosciences, Nanjing University, Nanjing 210093 (China); Yin Ying; Ji Rong [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Wu Jichun [State Key Laboratory of Pollution Control and Resource Reuse, Department of Hydrosciences, Nanjing University, Nanjing 210093 (China); Wang Xiaorong [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Guo Hongyan, E-mail: hyguo@nju.edu.cn [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China)

    2010-09-15

    In order to explore a practical approach to the remediation of the cadmium (Cd)-contaminated soil in the lower reaches of the Yangtze River, we evaluated the effects of a local willow (Salix x aureo-pendula CL 'J1011') of absorbing, accumulating, and translocating Cd; and assessed the potential of chelator ethylenediaminetetraacetic acid (EDTA) in combination with ethyl lactate for enhancing the efficiency of the willow in removing Cd in two water-culture growth chamber trials and a field one. The willow showed a high tolerance to Cd in growth chamber trial 1 where the Cd concentration in the medium reached up to 25 mg L{sup -1} medium, and the bioaccumulation factors (BAFs) of the shoots for Cd rose from 3.8 to 7.4 as the Cd concentration in the medium was elevated from 5 to 25 mg L{sup -1} medium. In growth chamber trial 2, the average Cd removal rates in two treatments with EDTA and ethyl lactate (molar ratios of EDTA to ethyl lactate = 68/39 and 53.5/53.5, respectively) reached 0.71 mg d{sup -1}pot{sup -1} for the duration of Day 5-8 and 0.59 mg d{sup -1}pot{sup -1} for that of Day 8-11, which were 5- and 4-fold of their counterparts in the control, respectively. In the field trial, for the remediational duration of 45 days, three treatments-willow alone, willow combined with EDTA, and willow combined with EDTA and ethyl lactate-led to decreases in the Cd concentration in soil by 5%, 20%, and 29%, respectively; increases in that in the leaves by 14.6%, 56.7%, and 146.5%, respectively; and increases in that in the stems by 15.6%, 41.2%, and 87.4%, respectively, compared to their counterparts on Day 0. These results indicate that EDTA combined with ethyl lactate significantly enhanced the efficiency of willow in removing Cd from the soil. Therefore, a phytoextration system consisting of the autochthonous willow, EDTA, and ethyl lactate has high potential for the remediation of the Cd-polluted soil in the lower reaches of the Yangtze River.

  20. Ethyl lactate-EDTA composite system enhances the remediation of the cadmium-contaminated soil by autochthonous willow (Salix x aureo-pendula CL 'J1011') in the lower reaches of the Yangtze River.

    Science.gov (United States)

    Li, Jiahua; Sun, Yuanyuan; Yin, Ying; Ji, Rong; Wu, Jichun; Wang, Xiaorong; Guo, Hongyan

    2010-09-15

    In order to explore a practical approach to the remediation of the cadmium (Cd)-contaminated soil in the lower reaches of the Yangtze River, we evaluated the effects of a local willow (Salix x aureo-pendula CL 'J1011') of absorbing, accumulating, and translocating Cd; and assessed the potential of chelator ethylenediaminetetraacetic acid (EDTA) in combination with ethyl lactate for enhancing the efficiency of the willow in removing Cd in two water-culture growth chamber trials and a field one. The willow showed a high tolerance to Cd in growth chamber trial 1 where the Cd concentration in the medium reached up to 25 mg L(-1) medium, and the bioaccumulation factors (BAFs) of the shoots for Cd rose from 3.8 to 7.4 as the Cd concentration in the medium was elevated from 5 to 25 mg L(-1) medium. In growth chamber trial 2, the average Cd removal rates in two treatments with EDTA and ethyl lactate (molar ratios of EDTA to ethyl lactate=68/39 and 53.5/53.5, respectively) reached 0.71 mg d(-1) pot(-1) for the duration of Day 5-8 and 0.59 mg d(-1) pot(-1) for that of Day 8-11, which were 5- and 4-fold of their counterparts in the control, respectively. In the field trial, for the remediational duration of 45 days, three treatments-willow alone, willow combined with EDTA, and willow combined with EDTA and ethyl lactate-led to decreases in the Cd concentration in soil by 5%, 20%, and 29%, respectively; increases in that in the leaves by 14.6%, 56.7%, and 146.5%, respectively; and increases in that in the stems by 15.6%, 41.2%, and 87.4%, respectively, compared to their counterparts on Day 0. These results indicate that EDTA combined with ethyl lactate significantly enhanced the efficiency of willow in removing Cd from the soil. Therefore, a phytoextration system consisting of the autochthonous willow, EDTA, and ethyl lactate has high potential for the remediation of the Cd-polluted soil in the lower reaches of the Yangtze River. Copyright 2010 Elsevier B.V. All rights

  1. [Enhanced phytoextraction of heavy metal contaminated soil by chelating agents and auxin indole-3-acetic acid].

    Science.gov (United States)

    Zhou, Jian-min; Dang, Zhi; Chen, Neng-chang; Xu, Sheng-guang; Xie, Zhi-yi

    2007-09-01

    The environmental risk of chelating agents such as EDTA application to the heavy metals polluted soils and the stress on plant roots due to the abrupt increase metals concentration limit the wide commercial use of chelate-induced phytoextraction. Chelating agent ethylenediaminetetraacetic acid (EDTA) and nitrilotriacetic acid (NTA) and auxin indole-3-acetic acid (IAA) were used for enhancing heavy metals uptake from soils by Zea mays L. (corn) in pot experiments. The metals content in plant tissues was quantified using an inductively coupled plasma mass spectrometer (ICP-MS). The results showed that the combination of IAA and EDTA increased the biomass by about 40.0% and the contents of Cu, Zn, Cd and Pb in corn shoots by 27.0%, 26.8%, 27.5% and 32.8% respectively, as compared to those in EDTA treatment. While NTA&IAA treatment increased the biomass by about 29.9% and the contents of Cu, Zn, Cd and Pb in corn shoots by 31.8%, 27.6%, 17.0% and 26.9% respectively, as compared to those in NTA treatment. These results indicated that corn growth was promoted, and the biomass and the accumulation of heavy metals in plant shoots were increased significantly with the addition of IAA, which probably helps to change the cell membrane properties and the biomass distribution, resulting in the alleviation of the phytotoxicity of metals and the chelating agents.

  2. A scanning electron microscopy study of diseased root surfaces conditioned with EDTA gel plus Cetavlon after scaling and root planing.

    Science.gov (United States)

    Martins Júnior, Walter; De Rossi, Andiara; Samih Georges Abi Rached, Ricardo; Rossi, Marcos Antonio

    2011-01-01

    In the present investigation, a scanning electron microscopy analysis was performed to evaluate the effects of the topical application of ethylenediaminetetraacetic acid (EDTA) gel associated with Cetavlon (EDTAC) in removing the smear layer and exposing collagen fibers following root surface instrumentation. Twenty-eight teeth from adult humans, single rooted and scheduled for extraction due to periodontal reasons, were selected. Each tooth was submitted to manual (scaling and root planing) instrumentation alone or combined with ultrasonic instruments, with or without etching using a 24% EDTAC gel. Following extraction, specimens were processed and examined under a scanning electron microscope. A comparative morphological semi-quantitative analysis was performed; the intensity of the smear layer and the decalcification of cementum and dentinal surfaces were graded in 12 sets using an arbitrary scale ranging from 1 (area covered by a smear layer) to 4 (no smear layer). Root debridement with hand instruments alone or combined with ultrasonic instruments resulted in a similar smear layer covering the root surfaces. The smear layer was successfully removed from the surfaces treated with EDTAC, which exhibited numerous exposed dentinal tubules and collagen fibers. This study supports the hypothesis that manual instrumentation alone or instrumentation combined with ultrasonic instrumentation is unable to remove the smear layer, whereas the subsequent topical application of EDTAC gel effectively removes the smear layer, uncovers dentinal openings and exposes collagen fibers.

  3. Hydroxyapatite synthesis using EDTA.

    Science.gov (United States)

    Kang, Nak Heon; Kim, Soon Je; Song, Seung Han; Choi, Sang mun; Choi, Sik Young; Kim, Youn Jung

    2013-05-01

    Bone comprises structure of the body and consisted of inorganic substances. It exists in an organic structure in the body. Even though it is firm and has self-healing mechanism, it can be damaged by trauma, cancer, or bone diseases. Allograft can be an alternative solution for autologous bone graft. Hydroxyapatite (Ca10(PO4)6(OH)2), an excellent candidate for allograft, can be applied to bone defect area. There are several methods to produce hydroxyapatite; however, economical cost and being time consuming make the production difficult. In this study, we synthesized hydroxyapatite with EDTA. Freeze-dried bone allograft (Hans Biomed) was used as the control group. Synthesized hydroxyapatite was a rod-shaped, white powdery substance with 2- to 5-μm length and 0.5- to 1-μm width. X-ray diffraction showed the highest sharp peak at 32°C and high peaks at 25.8°C, 39.8°C, 46.8°C, 49.5°C, and 64.0°C, indicating a similar substance to the freeze-dried bone allograft. After 3 days, the cell growth of synthesized hydroxyapatite showed 1.5-fold more than did the bone allograft. Cellular and media alkaline phosphate activity increased similar to the bone allograft. In this study, we came up with a new method to produce the hydroxyapatite. It is a convenient method that can be held in room temperature and low pressure. Also, the product can be manufactured in large quantity. It can be also transformed into scaffold structure, which will perform a stronger configuration. The manufacturing method will help the bony defect patients and make future medical products.

  4. Ion exchange behaviour of citrate and EDTA anions on strong and weak base organic ion exchangers

    International Nuclear Information System (INIS)

    Askarieh, M.M.; White, D.A.

    1988-01-01

    The exchange of citrate and EDTA ions with two strong base and two weak base exchangers is considered. Citrate and EDTA analysis for this work was performed using a colorimetric method developed here. The ions most selectively exchanged on the resins are H 2 cit - and H 2 EDTA 2- , though EDTA is generally less strongly sorbed on strong base resins. In contact with weak base resins, deprotonation of the resin occurs during ion exchange with a noticeable drop in solution pH. Although EDTA sorption can be reversed by nitric acid, citrate ions are significantly held on the resin at low pH. The exchange of citrate can be made reversible if bicarbonate is added to the initial solutions. Alkaline regeneration of exchangers loaded with EDTA proved to be very effective. (author)

  5. Kinetics of electrophilic exchange of lanthanide(3) and copper(2) ions in aqueous solutions of ethylenediaminetetraacetates

    International Nuclear Information System (INIS)

    Nikitenko, S.I.; Martynenko, L.I.; Pechurova, N.I.; Spitsyn, V.I.

    1984-01-01

    Electrophilic exchange in the MA - - Cu 2+ (M 3+ =La 3+ , P 2 3+ , Nd 3+ , Eu 3+ , Gd 3+ , Tb 3+ , Er 3+ , Yb 3+ , A 4- =ethylenediaminetetraacetate) systems is studied. Copper is chosen as a constituent cation, because the stability of the CuA 2+ - complex is higher than that of the MA - complex, besides the CuA 2- , is characterized by a high extinction coefficient E 280 =3200. Electrophilic exchange in the systems is shown to be realized through the acid dissociation mechanism and through the association mechanism. Consideration of the acid dissociation mechanism assumes that the exchange is limited by a break in the metal-oxygen bond in the intermediate protonated complex. In case of the association mechanism it is limited by a break in the metal-nitrogen bond in the intermediate binuclear complex. Rate constants of the limiting stage are calculated. Dependences of the rate constants of exchange on the stability of the complexes, the order number of the element, acidity of medium, Cu 2+ concentration are given. Differences are shown to exist between the exchange mechanisms in the studied: systems for the REE of cerium and yttrium subgroups

  6. Removal of Zn2+ from aqueous single metal solutions and electroplating wastewater with wood sawdust and sugarcane bagasse modified with EDTA dianhydride (EDTAD).

    Science.gov (United States)

    Pereira, Flaviane Vilela; Gurgel, Leandro Vinícius Alves; Gil, Laurent Frédéric

    2010-04-15

    This work describes the preparation of a new chelating material derived from wood sawdust, Manilkara sp., and not only the use of a new support, but also a chemically modified sugarcane bagasse synthesized in our previous work to remove Zn(2+) from aqueous solutions and electroplating wastewater. The first part describes the chemical modification of wood sawdust and sugarcane bagasse using ethylenediaminetetraacetic dianhydride (EDTAD) as modifying agent in order to introduce carboxylic acid and amine functional groups into these materials. The obtained materials such as the modified sugarcane bagasse, EB, and modified wood sawdust, ES were then characterized by infrared spectroscopy (IR) and CHN. The second part evaluates the adsorption capacity of Zn(2+) by EB and ES from aqueous single metal solutions and real electroplating wastewater, which concentration was determined through direct titration with EDTA and inductively coupled plasma (ICP-OES). Adsorption isotherms were developed using Langmuir model. Zn(2+) adsorption capacities were found to be 80 mg/g for ES and 105 mg/g for EB whereas for the industrial wastewater these values were found to be 47 mg/g for ES and 45 mg/g for EB. Zn(2+) adsorption in the wastewater was found to be lower than in Zn(2+) spiked solution due to the competition between other cations and/or interference of other ions, mainly Ca(2+) and Cl(-) that were present in the wastewater. 2009 Elsevier B.V. All rights reserved.

  7. EDTA modified glassy carbon electrode: Preparation and characterization

    Energy Technology Data Exchange (ETDEWEB)

    Ustuendag, Zafer [Dumlupinar University, Faculty of Arts and Sciences, Department of Chemistry, Kuetahya (Turkey); Solak, Ali Osman [Ankara University, Faculty of Science, Department of Chemistry, Degol Street, Tandogan, 06100 Ankara (Turkey)], E-mail: osolak@science.ankara.edu.tr

    2009-11-01

    EDTA-phenoxyamide modified glassy carbon electrode (EDTA-GC) was prepared at a glassy carbon electrode by surface synthesis. In the first step, nitrophenyl was grafted to the glassy carbon (GC) surface via the electrochemical reduction of its tetraflouroborate diazonium salt. In the second step, nitrophenyl-modified electrode (NP-GC) was subjected to the cathodic potential scan to reduce the nitro to amine group. p-Aminophenyl modified glassy carbon electrode (AP-GC) was dipped into a EDTA solution containing 1-ethyl-3(3-(dimethlyamino)propyl)-carbodiimide (EDC) as an activating agent. Thus formed ((2-anilino-2-oxoethyl){l_brace}2-[bis(carboxymethyl)amino]-ethyl{r_brace}amino)acetic acid modified GC electrode was denoted as EDTA-GC and characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), ellipsometry and X-ray photoelectron spectroscopy (XPS). Complexation of the EDTA-GC surface with Pb{sup 2+} ions was investigated if this electrode could be used as a metal sensor.

  8. Effect of edta on arsenic phytoextraction by arundo donax

    International Nuclear Information System (INIS)

    Mirza, N.; Pervez, A.; Mahmood, Q.; Shah, M.M.; Farooq, U.

    2014-01-01

    Ligand assisted metal uptake by plants is recent trend in environmental clean-up. Arundo donax L. has been demonstrated as a suitable bioresource for the phytoextraction of arsenic recently. A. donax L. plants were grown in arsenic contaminated soil with doses (5 and 10 mg kg/sup -1/) of Ethylene Diamine Tetra Acetic Acid (EDTA) to investigate chelator-assisted phytoextraction. The arsenic treatments included control (no metal) and five doses of arsenic, i.e., 50, 100, 300, 600 and 1000 micro g kg/sup -1/ . The arsenic concentrations linearly increased in parts of plant with the increasing arsenic and EDTA in growth medium. Ligand addition also resulted in the increased arsenic accumulation in the shoot over its control plants. EDTA additionat the rate 5 mg kg/sup -1/ to the treatment system may effectively increase the arsenic uptake by A. donax without severe growth suppression. (author)

  9. Interaction of ruthenium (4) and osmium (4) with 2-mercaptobenzimidazole, 2-mercaptobenzoxazole and 2-mercaptobenzothiazole in the presence of EDTA

    Energy Technology Data Exchange (ETDEWEB)

    Busev, A I; Ignat' eva, T I; Lomakina, L N [Moskovskij Gosudarstvennyj Univ. (USSR). Kafedra Analiticheskoj Khimii

    1975-05-01

    Interaction of ruthenium (4) and osmium (4) with 2-mercaptobenzimidazole (2MBI), 2-mercaptobenzoxazole (2MBO) and 2-mercaptobenzothiazole (2MBT) in the presence of EDTA was studied. The interaction of ruthenium (4) and osmium (4) with EDTA was considered. Ruthenium complex is formed with constant output at 2-4.5 pH after 30 min. heating. In the low acid solution (pH 2-4) osmium reacts with EDTA forming soluble compound. Characteristics of ruthenium (4) compound with 2MBI, 2MBO and 2MBT produced in the presence of EDTA are presented. Osmium (4) in the presence of EDTA and above mentioned organic reagents and when heating forms low soluble compounds. Possibility of joint determination of ruthenium and osmium with help of 2MBI in the presence of EDTA under conditions of minimum complexing osmium with EDTA was investigated.

  10. Effect of low-molecular-weight organic acids on photo-degradation of phenanthrene catalyzed by Fe(III)-smectite under visible light.

    Science.gov (United States)

    Jia, Hanzhong; Chen, Hongxia; Nulaji, Gulimire; Li, Xiyou; Wang, Chuanyi

    2015-11-01

    The photolysis of polycyclic aromatic hydrocarbons (PAHs) is potentially an important process for its transformation and fate on contaminated soil surfaces. In this study, phenanthrene is employed as a model to explore PAH photodegradation with the assistance of Fe(III)-smectite under visible-light while focusing on roles played by five low-molecular-weight organic acids (LMWOAs), i.e., malic acid, oxalic acid, citric acid, ethylenediaminetetraacetic acid (EDTA), and nitrilotriacetic acid. Our results show that oxalic acid is most effective in promoting the photodegradation of phenanthrene, while only a slight increase in the rate of phenanthrene photodegradation is observed in the presence of malic acid. Electron paramagnetic resonance experiments confirm the formation of CO2(-) radicals in the presence of malic and oxalic acid, which provides strong evidence for generating OH and subsequent photoreaction pathways. The presence of EDTA or nitrilotriacetic acid significantly inhibits both Fe(II) formation and phenanthrene photodegradation because these organic anions tend to chelate with Fe(III), leading to decreases in the electron-accepting potential of Fe(III)-smectite and a weakened interaction between phenanthrene and Fe(III)-smectite. These observations provide valuable insights into the transformation and fate of PAHs in the natural soil environment and demonstrate the potential for using some LMWOAs as additives for the remediation of contaminated soil. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Application of a modified BCR sequential extraction (three-step) procedure for the determination of extractable trace metal contents in a sewage sludge amended soil reference material (CRM 483), complemented by a three-year stability study of acetic acid and EDTA extractable metal content.

    Science.gov (United States)

    Rauret, G; López-Sánchez, J F; Sahuquillo, A; Barahona, E; Lachica, M; Ure, A M; Davidson, C M; Gomez, A; Lück, D; Bacon, J; Yli-Halla, M; Muntau, H; Quevauviller, P

    2000-06-01

    This paper provides additional data on a sewage sludge amended soil certified reference material, CRM 483, which was certified in 1997 for its EDTA and acetic acid extractable contents of some trace metals, following standardised extraction procedures. The additional work aimed to test the long-term stability of the material and the applicability of an improved version of the BCR three-step sequential extraction procedure on the sewage sludge amended soil (CRM 483). The paper demonstrates the CRM 483 long-term stability for EDTA and acetic acid extractable contents of Cd, Cr, Cu, Ni, Pb and Zn and gives the results (obtained in the framework of an interlaboratory study) for the extractable contents of the same elements in the CRM 483, following the BCR three-step sequential extraction scheme. The aqua regia extractable contents following the ISO 11466 Standard are also given. The data are given as indicative (not certified) values.

  12. Hydroxyapatite synthesis using EDTA

    Science.gov (United States)

    Kang, Nak Heon; Kim, Soon Je; Song, Seung Han; Choi, Sang mun; Choi, Sik Young; Kim, Youn Jung

    2013-01-01

    Bone comprises structure of body and is consisted of inorganic substances. It exists in an organic structure in the body. Even though it is firm and has self healing mechanism, it can be damaged by trauma, cancer, or bone diseases. Allograft can be an alternative solution for autologous bone graft. Hydroxyapatite(Ca10(PO4)6(OH)2), an excellent candidate for allograft, can be applied to bone defect area. There are several methods to produce hydroxyapatite, however economical cost and time consuming make the production difficult. In this study we synthesized the hydroxyapatite with Ethyenediamine tetraacetic acid. Freeze Dried Bone Allograft(Hans Biomed) was used to be a control group. Synthesized hydroxyapatite was a rod shape, white powdery type substance with 2 ~ 5 μm length and 0.5 ~ 1 μm width. X-ray diffraction showed the highest sharp peak at 32° and high peaks at 25.8°, 39.8°, 46.8°, 49.5°, and 64.0° indicating a similar substance to the freeze Dried Bone Allograft. 3 days after the cell growth of synthesized hydroxyapatite showed 1.5 fold more than the Bone Allograft. Cellular and media alkaline phosphate activity increased similar to the bone alloagraft. In this study we came up with a new method to produce the hydroxyapatite. It is a convenient method that can be held in room temperature and low pressure. Also the the product can be manufactured in large quantity. It can be also transformed into scaffold structure which will perform a stronger configuration. The manufacturing method will help the bony defect patients and make future medical products. PMID:23714942

  13. Enhancing phosphorus release from waste activated sludge containing ferric or aluminum phosphates by EDTA addition during anaerobic fermentation process.

    Science.gov (United States)

    Zou, Jinte; Zhang, Lili; Wang, Lin; Li, Yongmei

    2017-03-01

    The effect of ethylene diamine tetraacetic acid (EDTA) addition on phosphorus release from biosolids and phosphate precipitates during anaerobic fermentation was investigated. Meanwhile, the impact of EDTA addition on the anaerobic fermentation process was revealed. The results indicate that EDTA addition significantly enhanced the release of phosphorus from biosolids, ferric phosphate precipitate and aluminum phosphate precipitate during anaerobic fermentation, which is attributed to the complexation of metal ions and damage of cell membrane caused by EDTA. With the optimal EDTA addition of 19.5 mM (0.41 gEDTA/gSS), phosphorus release efficiency from biosolids was 82%, which was much higher than that (40%) without EDTA addition. Meanwhile, with 19.5 mM EDTA addition, almost all the phosphorus in ferric phosphate precipitate was released, while only 57% of phosphorus in aluminum phosphate precipitate was released. This indicates that phosphorus in ferric phosphate precipitate was much easier to be released than that in aluminum phosphate precipitate during anaerobic fermentation of sludge. In addition, proper EDTA addition facilitated the production of soluble total organic carbon and volatile fatty acids, as well as solid reduction during sludge fermentation, although methane production could be inhibited. Therefore, EDTA addition can be used as an alternative method for recovering phosphorus from waste activated sludge containing ferric or aluminum precipitates, as well as recovery of soluble carbon source. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. Transport of EDTA into cells of the EDTA-degrading bacterial strain DSM 9103.

    Science.gov (United States)

    Witschel, M; Egli, T; Zehnder, A J; Wehrli, E; Spycher, M

    1999-04-01

    In the bacterial strain DSM 9103, which is able to grow with the complexing agent EDTA as the sole source of carbon, nitrogen and energy, the transport of EDTA into whole cells was investigated. EDTA uptake was found to be dependent on speciation: free EDTA and metal-EDTA complexes with low stability constants were readily taken up, whereas those with stability constants higher than 1016 were not transported. In EDTA-grown cells, initial transport rates of CaEDTA showed substrate-saturation kinetics with a high apparent affinity for CaEDTA (affinity constant Kt= 0.39 microM). Several uncouplers had an inhibitory effect on CaEDTA transport. CaEDTA uptake was also significantly reduced in the presence of an inhibitor of ATPase and the ionophore nigericin, which dissipates the proton gradient. Valinomycin, however, which affects the electrical potential, had little effect on uptake, indicating that EDTA transport is probably driven by the proton gradient. Of various structurally related compounds tested only Ca2+-complexed diethylenetriaminepentaacetate (CaDTPA) competitively inhibited CaEDTA transport. Uptake in fumarate-grown cells was low compared to that measured in EDTA-grown bacteria. These results strongly suggest that the first step in EDTA degradation by strain DSM 9103 consists of transport by an inducible energy-dependent carrier. Uptake experiments with 45Ca2+ in the presence and absence of EDTA indicated that Ca2+ is transported together with EDTA into the cells. In addition, these transport studies and electron-dispersive X-ray analysis of electron-dense intracellular bodies present in EDTA-grown cells suggest that two mechanisms acting simultaneously allow the cells to cope with the large amounts of metal ions taken up together with EDTA. In one mechanism the metal ions are excreted, in the other they are inactivated intracellularly in polyphosphate granules.

  15. Development of an eco-friendly material recycling process for spent lead glass using a mechanochemical process and Na2EDTA reagent.

    Science.gov (United States)

    Sasai, Ryo; Kubo, Hisashi; Kamiya, Masahiro; Itoh, Hideaki

    2008-06-01

    To develop a novel nonheating method with lower energy consumption and higher efficiency for recovering both lead and SiO2 glass matrix from spent lead-glass powder, we attempted to treat the spent lead glass by the mechanochemical method using the metal chelate reagent, sodium ethylenediaminetetraacetate (Na2EDTA). As a result of the wet ball-milling treatment of spent lead-glass powder sealed in a polypropylene bottle with zirconia balls, Na2EDTA, and water at room temperature, we found that more than 99 mass % of lead contained in the spentlead-glass powder was extracted as a lead-EDTA species from the solid silica glass network matrix. This separation phenomenon was accelerated by the enlargement of the solid-liquid interface area due to ball-milling atomization and by the high stability constant of lead-EDTA. High extraction yield suggests that Pb-O-Pb bonds in lead glass are weakened or are broken down by the wet ball-milling treatment, i.e., the strong mechanical energy such as the potential and/ or friction energy provided by ball-milling may be high enough to elute lead ions from silica matrix. Moreover, we succeeded in recovering both lead ions as lead sulfate, which is the main compound of anglesite, and the EDTA as sodium-EDTA, which is reusable as the metal chelate reagent in wet chemical process using the ferric sulfate.

  16. Effect of EDTA Conditioning on Microleakage of Four Adhesive Systems in Composite Restorations

    Directory of Open Access Journals (Sweden)

    F. Shafiei

    2008-12-01

    Full Text Available Objective: Evaluating the effect of dentin conditioning with EDTA on microleakage of composite resin restorations, using two etch and rinse and two self-etch adhesives.Materials and Methods: One hundred and sixty extracted molars received class V cavity preparations right under the CEJ and were randomly divided into eight groups of 20, usingfour different adhesive systems. These adhesives included Adper Scotchbond Multi-purpose (SBMP, Adper Single Bond (SB, Clearfil SE Bond (CSEB, and Adper Prompt L-Pop (PLP. In the SBMP and SB experimental groups, EDTA was applied instead of phosphoric acid. In the CSEB and PLP experimental groups, EDTA conditioning was added to the bonding process. After thermocycling, the amount of dye penetration was evaluated using stereomicroscope. The data were analyzed using the Kruskal-Wallis and Dunn tests.Results: Two etch and rinse adhesives (SBMP, SB showed a significantly lower micro-leakage than the two self-etch adhesives, CSEB and PLP, (P<0.05. No significant differ-ence was observed among the experimental groups. PLP and CSEB showed significantly less microleakage using EDTA conditioning (P<0.05. There was no significant difference for SBMP and SB when applying either phosphoric acid or EDTA.Conclusion: In the cases of SBMP and SB, EDTA conditioning is as effective as phos-phoric acid in preventing microleakage. In cases of CSEB and PLP, EDTA conditioning can significantly improve the sealing ability.

  17. Assessment of canine intestinal permeability, using 51Cr-labeled ethylenediaminetetraacetate

    International Nuclear Information System (INIS)

    Hall, E.J.; Batt, R.M.; Brown, A.

    1989-01-01

    The 51 Cr-labeled EDTA was validated as a suitable permeability probe in dogs for measurement of passive, unmediated diffusion across intestinal mucosa via intercellular pathways. The 51 Cr-labeled EDTA was stable in aqueous solution and did not bind to biologic tissue and fluids. After incubation of 51 Cr-labeled EDTA in isolated jejunal loops, analytic subcellular fractionation of jejunal mucosa on reorientating sucrose-density gradients was performed, and no association of 51 Cr-labeled EDTA with particulate intracellular organelles was detected. Intravenously administered 51 Cr-labeled EDTA was rapidly and completely excreted in urine. Intestinal permeability to 51 Cr-labeled EDTA after oral administration was assessed in healthy dogs. The percentage of the administered dose of 51 Cr-labeled EDTA excreted in the urine in 24 hours ranged from 2.3 to 17.6% (median, 13%)

  18. Comparative study on the mechanisms of rotavirus inactivation by sodium dodecyl sulfate and ethylenediaminetetraacetate

    Energy Technology Data Exchange (ETDEWEB)

    Ward, R.L. (Sandia Labs., Albuquerque, NM); Ashley, C.S.

    1980-06-01

    This report describes a comparative study on the effects of the anionic detergent sodium dodecyl sulfate and the chelating agent ethylenediaminetetraacetate on purified rotavirus SA-11 particles. Both chemicals readily inactivated rotavirus at quite low concentrations and under very mild conditions. In addition, both agents modified the viral capsid and prevented the adsorption of inactivated virions to cells. Capsid damage by ethylenediaminetetraacetate caused a shift in the densities of rotavirions from about l.35 to about 1.37 g/ml and a reduction in their sedimentation coefficients. Sodium dodcyl sulfate, on the other hand, did not detectably alter either of these physical properties of rotavirions. Both agents caused some alteration of the isoelectric points of the virions. Finally, analysis of rotavirus proteins showed that ethylenediaminetetraacetate caused the loss of two protein peaks from the electrophoretic pattern of virions but sodium dodecyl sulfate caused the loss of only one of these same protein peaks.

  19. EDTA chelation therapy alone and in combination with oral high-dose multivitamins and minerals for coronary disease: The factorial group results of the Trial to Assess Chelation Therapy.

    Science.gov (United States)

    Lamas, Gervasio A; Boineau, Robin; Goertz, Christine; Mark, Daniel B; Rosenberg, Yves; Stylianou, Mario; Rozema, Theodore; Nahin, Richard L; Terry Chappell, L; Lindblad, Lauren; Lewis, Eldrin F; Drisko, Jeanne; Lee, Kerry L

    2014-07-01

    Disodium ethylenediaminetetraacetic acid (EDTA) reduced adverse cardiac outcomes in a factorial trial also testing oral vitamins. This report describes the intent-to-treat comparison of the 4 factorial groups overall and in patients with diabetes. This was a double-blind, placebo-controlled, 2 × 2 factorial multicenter randomized trial of 1,708 post-myocardial infarction (MI) patients ≥50 years of age and with creatinine ≤2.0 mg/dL randomized to receive 40 EDTA chelation or placebo infusions plus 6 caplets daily of a 28-component multivitamin-multimineral mixture or placebo. The primary end point was a composite of total mortality, MI, stroke, coronary revascularization, or hospitalization for angina. Median age was 65 years, 18% were female, 94% were Caucasian, 37% were diabetic, 83% had prior coronary revascularization, and 73% were on statins. Five-year Kaplan-Meier estimates for the primary end point was 31.9% in the chelation + high-dose vitamin group, 33.7% in the chelation + placebo vitamin group, 36.6% in the placebo infusion + active vitamin group, and 40.2% in the placebo infusions + placebo vitamin group. The reduction in primary end point by double active treatment compared with double placebo was significant (hazard ratio 0.74, 95% CI 0.57-0.95, P = .016). In patients with diabetes, the primary end point reduction of double active compared with double placebo was more pronounced (hazard ratio 0.49, 95% CI 0.33-0.75, P < .001). In stable post-MI patients on evidence-based medical therapy, the combination of oral high-dose vitamins and chelation therapy compared with double placebo reduced clinically important cardiovascular events to an extent that was both statistically significant and of potential clinical relevance. Copyright © 2014 The Authors. Published by Mosby, Inc. All rights reserved.

  20. Effects of Na4EDTA and EDTA on seeded precipitation of sodium aluminate solution

    Institute of Scientific and Technical Information of China (English)

    吕保林; 陈启元; 尹周澜; 胡慧萍

    2010-01-01

    Na4EDTA and EDTA were adopted as new additives to intensify the seeded precipitation process of sodium aluminate solution. The effects of the two additives at certain concentrations on the seeded precipitation rate of sodium aluminate solution, particle size distribution (PSD) and morphology of precipitated gibbsite were investigated using titration method, particle size analyzer and scanning electron microscope (SEM), respectively. The results show that the two additives can accelerate the seeded precipitation rate of sodium aluminate solution. At relatively high concentration, the facilitative effect of EDTA on sodium aluminate solution is more obvious than that of Na4EDTA. EDTA makes gibbsite particles thinner than Na4EDTA. The Na+ and H+ result in the different effects on the seeded precipitation rate of sodium aluminate solution in spite of the same EDTA anion in the two additives.

  1. Evaluation of deoxyribonucleic acid (DNA) isolated from human bloodstains exposed to ultraviolet light, heat, humidity, and soil contamination

    International Nuclear Information System (INIS)

    McNally, L.; Shaler, R.C.; Baird, M.; Balazs, I.; De Forest, P.; Kobilinsky, L.

    1989-01-01

    This study was designed to analyze the effects of common environmental insults on the ability to obtain deoxyribonucleic acid (DNA) restriction fragment-length polymorphisms (RFLP) patterns from laboratory prepared specimens. The environmental conditions studied include the exposure of dried bloodstains to varying amounts of relative humidity (0, 33, 67, and 98%), heat (37 degree C), and ultraviolet light for periods of up to five days. In addition, the effect of drying over a four-day period in whole blood collected with and without ethylenediaminetetraacetate (EDTA) was examined. The results of the study showed that, under the conditions studied, the integrity of DNA is not altered such that false RFLP patterns are obtained. The only effect observed was that the overall RFLP pattern becomes weaker, but individual RFLP fragments are neither created nor destroyed

  2. Concurrent determination of total serum calcium and magnesium by thermometric titration with ethylenediaminetetraacetate.

    Science.gov (United States)

    Callicott, R H; Carr, P W

    1976-07-01

    Total serum calcium and magnesium may be determined in one thermometric titration, with disodium ethylenediaminetetraacetate as the titrant. A 1-ml serum sample is diluted with 1 ml of tris(hydroxymethyl)aminomethane buffer (pH 8) and titrated at a constant rate with a motorized syringe buret. Results by the thermometric method compared well with those by atomic absorption spectroscopy.

  3. Effect of EDTA on luminescence property of Eu+3 doped YPO4 nanoparticles

    International Nuclear Information System (INIS)

    Parchur, A.K.; Okram, G.S.; Singh, R.A.; Tewari, R.; Pradhan, Lina; Vatsa, R.K.; Ningthoujan, R.S.

    2010-01-01

    Nanoparticles of Eu 3+ doped YPO 4 have been prepared using ethylene glycol (EG). Ethylene diamine tetra acetic acid (EDTA) is used as a complexing agent. X-ray diffraction results show that the nanoparticles are crystalline in tetragonal structure. Based on William-Hall relation, the effective crystallite size and strain developed in lattice are found to be 28 nm and 0.002, respectively. With the addition of EDTA, there is a slight shift towards the lower wavelength in emission peaks. Asymmetric ratio of electric to magnetic dipole transition intensities are found to decrease with addition of EDTA. Emission intensity decreases with EDTA because of decrease of particle size as well as decrease of number of Eu 3+ activators per unit volume. These materials are dispersible in water, which may have potential biological applications. (author)

  4. Effect Of EDTA On Luminescence Property Of Eu+3 Doped YPO4 Nanoparticles

    International Nuclear Information System (INIS)

    Parchur, A. K.; Okram, G. S.; Singh, R. A.; Tewari, R.; Pradhan, Lina; Vatsa, R. K.; Ningthoujam, R. S.

    2010-01-01

    Nanoparticles of Eu 3+ doped YPO 4 have been prepared using ethylene glycol (EG). Ethylene diamine tetra acetic acid (EDTA) is used as a complexing agent. X-ray diffraction results show that the nanoparticles are crystalline in tetragonal structure. Based on William-Hall relation, the effective crystallite size and strain developed in lattice are found to be 28 nm and 0.002, respectively. With the addition of EDTA, there is a slight shift towards the lower wavelength in emission peaks. Asymmetric ratio of electric to magnetic dipole transition intensities are found to decrease with addition of EDTA. Emission intensity decreases with EDTA because of decrease of particle size as well as decrease of number of Eu 3+ activators per unit volume. These materials are dispersible in water, which may have potential biological applications.

  5. Zinc species distribution in EDTA-extract residues of zinc-contaminated soil

    International Nuclear Information System (INIS)

    Chang, S.-H.; Wei, Y.-L.; Wang, H. Paul

    2007-01-01

    Soil sample from a site heavily contaminated with >10 wt.% zinc is sampled and extracted with aqueous solutions of ethylene diamine tetra-acetic acid (EDTA) that is a reagent frequently used to extract heavy metals in soil remediation. Three liquid/soil ratios (5/1, 20/1, and 100/1) were used in the extracting experiment. The molecular environment of the residual Zn in the EDTA-extract residues of zinc-contaminated soil is investigated with XANES technique. The results indicate that EDTA does not show considerable preference of chelating for any particular Zn species during the extraction. Zn species distribution in the sampled soil is found to resemble that in all EDTA-extract residues; Zn(OH) 2 is determined as the major zinc species (60-70%), seconded by organic zinc (21-26%) and zinc oxide (9-14%)

  6. Comparison of EDTA and EDDS as potential soil amendments for enhanced phytoextraction of heavy metals.

    Science.gov (United States)

    Meers, E; Ruttens, A; Hopgood, M J; Samson, D; Tack, F M G

    2005-02-01

    Phytoextraction has been proposed as an alternative remediation technology for soils polluted with heavy metals or radionuclides, but is generally conceived as too slow working. Enhancing the accumulation of trace pollutants in harvestable plant tissues is a prerequisite for the technology to be practically applicable. The chelating aminopolycarboxylic acid, ethylene diamine tetraacetate (EDTA), has been found to enhance shoot accumulation of heavy metals. However, the use of EDTA in phytoextraction may not be suitable due to its high environmental persistence, which may lead to groundwater contamination. This paper aims to assess whether ethylene diamine disuccinate (EDDS), a biodegradable chelator, can be used for enhanced phytoextraction purposes. A laboratory experiment was conducted to examine mobilisation of Cd, Cu, Cr, Ni, Pb and Zn into the soil solution upon application of EDTA or EDDS. The longevity of the induced mobilisation was monitored for a period of 40 days after application. Estimated effect half lives ranged between 3.8 and 7.5 days for EDDS, depending on the applied dose. The minimum observed effect half life of EDTA was 36 days, while for the highest applied dose no decrease was observed throughout the 40 day period of the mobilisation experiment. Performance of EDTA and EDDS for phytoextraction was evaluated by application to Helianthus annuus. Two other potential chelators, known for their biodegradability in comparison to EDTA, were tested in the plant experiment: nitrilo acetic acid (NTA) and citric acid. Uptake of heavy metals was higher in EDDS-treated pots than in EDTA-treated pots. The effects were still considered insufficiently high to consider efficient remediation. This may be partly due to the choice of timing for application of the soil amendment. Fixing the time of application at an earlier point before harvest may yield better results. NTA and citric acid induced no significant effects on heavy metal uptake.

  7. Calcium EDTA toxicity: renal excretion of endogenous trace metals and the effect of repletion on collagen degradation in the rat.

    Science.gov (United States)

    Braide, V B

    1984-01-01

    Studies on total hydroxyproline concentrations in urine of rats infused with toxic doses of CaEDTA at 6 mmol/kg per 24 hr for 48 hr or injected i.p. with the chelate at 4.8 mmol/kg/day for 10 days, indicate a two- to six-fold increase in urine excretion of the imino acid. This is due to increased degradation of collagen induced by CaEDTA. CaEDTA infusion was also shown to enhance urine excretion of some trace metals (Zn, Mn, Cu and Fe). Rats infused with CaEDTA for 36 hr showed a gradual fall in concentration of hydroxyproline in the urine, following cessation of chelate infusion. The decline in hydroxyproline concentrations was faster in rats receiving trace metal (Zn, Co, Mn or Ni) treatment during the post-CaEDTA infusion period; suggesting that the metals may affect collage, making the protein less susceptible to degradation in the body.

  8. Oxidation of aqueous EDTA and associated organics and coprecipitation of inorganics by ambient iron-mediated aeration.

    Science.gov (United States)

    Englehardt, James D; Meeroff, Daniel E; Echegoyen, Luis; Deng, Yang; Raymo, Françisco M; Shibata, Tomoyuki

    2007-01-01

    Cationic metal and radionuclide contaminants can be extracted from soils to groundwater with sequestering agents such as EDTA. However, EDTA must then be removed fromthe groundwater, by advanced oxidation or specialized biological treatment. In this work, aqueous individual metal-EDTA solutions were aerated with steel wool for 25 h, at ambient pH, temperature, and pressure. Removal of approximately 99% of EDTA (0.09-1.78 mM); glyoxylic acid (0.153 mM); chelated Cd2+ (0.94 and 0.0952 mM), Pb2+ (0.0502 mM), and Hg2+ (0.0419 mM); and free chromate and vanadate was shown. EDTA was oxidized to glyoxylic acid and formaldehyde, and metals/metalloids were coprecipitated together with iron oxyhydroxide floc. Free arsenite and arsenate were each removed at 99.97%. Free Sr2+, and chelated Ni2+ were removed at 92% and 63%, respectively. Similar removals were obtained from mixtures, including 99.996+/-0.004% removal of total arsenic (95% confidence). Traces of iminodiacetic acid, nitrilotriacetic acid, and ethylenediaminetriacetic acid were detected after 25 h. Results are consistent with first-order, solution-phase oxidation of EDTA and glyoxylic acid by ferryl ion and H202, respectively, with inhibition due to sludge accumulation, and equilibrium metal coprecipitation. This ambient process, to our knowledge previously unknown, agrees with recently reported findings and shows promise for remediation of metals, metalloids, and radionuclides in wastewater, soil, and sediment.

  9. Rates of nickel(II) capture from complexes with NTA, EDDA, and related tetradentate chelating agents by the hexadentate chelating agents EDTA and CDTA: Evidence of a "semijunctive" ligand exchange pathway

    Science.gov (United States)

    Boland, Nathan E.; Stone, Alan T.

    2017-09-01

    Many siderophores and metallophores produced by soil organisms, as well as anthropogenic chelating agent soil amendments, rely upon amine and carboxylate Lewis base groups for metal ion binding. UV-visible spectra of metal ion-chelating agent complexes are often similar and, as a consequence, whole-sample absorbance measurements are an unreliable means of monitoring the progress of exchange reactions. In the present work, we employ capillary electrophoresis to physically separate Ni(II)-tetradentate chelating agent complexes (NiL) from Ni(II)-hexadentate chelating agent complexes (NiY) prior to UV detection, such that progress of the reaction NiL + Y → NiY + L can be conveniently monitored. Rates of ligand exchange for Ni(II) are lower than for other +II transition metal ions. Ni(II) speciation in environmental media is often under kinetic rather than equilibrium control. Nitrilotriacetic acid (NTA), with three carboxylate groups all tethered to a central amine Lewis base group, ethylenediamine-N,N‧-diacetic acid (EDDA), with carboxylate-amine-amine-carboxylate groups arranged linearly, plus four structurally related compounds, are used as tetradentate chelating agents. Ethylenediaminetetraacetic acid (EDTA) and the structurally more rigid analog trans-cyclohexaneethylenediaminetetraacetic acid (CDTA) are used as hexadentate chelating agents. Effects of pH and reactant concentration are explored. Ni(II) capture by EDTA was consistently more than an order of magnitude faster than capture by CDTA, and too fast to quantify using our capillary electrophoresis-based technique. Using NiNTA as a reactant, Ni(II) capture by CDTA is independent of CDTA concentration and greatly enhanced by a proton-catalyzed pathway at low pH. Using NiEDDA as reactant, Ni(II) capture by CDTA is first order with respect to CDTA concentration, and the contribution from the proton-catalyzed pathway diminished by CDTA protonation. While the convention is to assign either a disjunctive

  10. Reduction of mercury from mackerel fillet using combined solution of cysteine, EDTA, and sodium chloride.

    Science.gov (United States)

    Hajeb, P; Jinap, S

    2012-06-13

    An acidic solution containing mercury chelating agents to eliminate mercury in raw fish (mackerel) fillet was developed. The solution contained hydrochloric acid, sodium hydroxide, cysteine, EDTA, and NaCl. The optimum conditions for mercury reduction were achieved using response surface methodology (RSM) at cysteine concentration of 1.25%, EDTA of 275 mg/L, NaCl of 0.5%, pH of 3.75, and exposure time of 18 min. The optimized conditions produced a solution which can remove up to 91% mercury from raw fish fillet. Cysteine and EDTA were identified as potential chelating agents with the greatest potential for use. The solution can be employed in fish industries to reduce mercury in highly contaminated fish.

  11. Crystal structure of strontium aqua(ethylenediaminetetraacetato)cobaltate(II) tetrahydrate Sr[CoEdta(H2O)] · 4H2O

    International Nuclear Information System (INIS)

    Zasurskaya, L.A.; Polynova, T.N.; Polyakova, I.N.; Sergienko, V.S.; Poznyak, A.L.

    2001-01-01

    The complex Sr[Co II Edta] · 5H 2 O (I) (where Edta 4- is the ethylenediaminetetraacetate ion) has been synthesized. The crystal structure of this compound is determined by X-ray diffraction. Crystals are monoclinic, a = 7.906(2) A, b = 12.768(2) A, c = 18.254(3) A, β = 95.30(3) deg., V 1834.8 A 3 , space group P2 1 /n, Z = 4, and R = 0.036. The structure is built up of the binuclear complex fragments {Sr(H 2 O) 3 [CoEdta(H 2 O)]}, which consist of the anionic [CoEdta(H 2 O)] 2- and cationic [Sr(H 2 O) 3 ] 2+ units linked by the Sr-O bonds into a three-dimensional framework. The coordination polyhedra of the Co and Sr atoms are mono- and bicapped trigonal prisms. The coordination sphere of the Co atom (the coordination number is equal to 6 + 1) involves six donor atoms (2N and 4O) of the Edta 4- ligand and the O w atom of water molecule. One of the Co-O distances (2.718 A) is considerably longer than the other Co-O lig distances (2.092-2.190 A) and the Co-O w (1) distance (2.079 A). The Sr coordination polyhedron (the coordination number is eight) contains three water molecules, three carbonyl O atoms of the three different anionic complexes, and two O atoms of one acetate group of the fourth anionic complex. The Sr-O distances fall in the range 2.535-2.674 A. The structural formula of the compound is {Sr(H 2 O) 3 [CoEdta(H 2 O)]} 3∞ · H 2 O

  12. Application of EDTA decontamination on soils affected by mining activities and impact of treatment on the geochemical partition of metal contaminants.

    Science.gov (United States)

    Xia, Wenbin; Gao, Hui; Wang, Xianhai; Zhou, Chunhua; Liu, Yunguo; Fan, Ting; Wang, Xin

    2009-05-30

    Two soil samples were collected at mining areas located in southern Hunan Province, China. EDTA extraction of Pb, Zn, Cu and Cd from these two tailing soils was studied using column leaching experiments. The redistributions of heavy metals (HMs) were determined using the modified BCR (Community Bureau of Reference) sequential extraction procedure, before and after EDTA extraction. The results indicated that EDTA was an effective extractant because of its strong chelating ability for various HMs. The proportions of Pb, Zn, Cu and Cd in the four fractions varied largely after EDTA extraction. The extraction efficiency of EDTA of the acid-extractable fraction (AEX) was significant in shallow soil column, while in deeper soil column, decrease of the extraction efficiency of reduced (RED), oxidizable (OX) and residual fractions (RES) was obtained, which was mainly due to the decrease of EDTA concentration.

  13. Carbon dioxide sorption on EDTA modified halloysite

    Directory of Open Access Journals (Sweden)

    Waszczuk Patrycja

    2016-01-01

    Full Text Available In this paper the sorption study of CO2 on EDTA surface modified halloysite was conducted. In the paper chemical modification of halloysite from the Dunino deposit (Poland and its influence on sorption of CO2 are presented. A halloysite samples were washed with water-EDTA 1% solution, centrifuged to separate liquid and impurities and dried. The samples were tested for the sorption capacity using a manometric method with pressure up to 3 MPa. A Langmuir adsorption model was fitted to the data. The results showed that EDTA had a limited effect on the increase of sorption potential at low pressure and the samples exhibited similar results to that ones treated solely with the water solution.

  14. Anaerobic Biotransformation and Mobility of Pu and PuEDTA

    International Nuclear Information System (INIS)

    Xun, Luying

    2005-01-01

    The objective of this report is to isolate anaerobic EDTA-degrading bacteria. Although our goal is to isolate anaerobic EDTA degraders, we initiated the experiments to include nitrilotriacetate (NTA), which is a structure homologue of EDTA. All the aerobic EDTA degraders can degrade NTA, but the isolated NTA degraders cannot degrade EDTA. Since NTA is a simpler structure homologue, it is likely that EDTA-degrading ability is evolved from NTA degradation. This hypothesis is further supported from our characterization of EDTA and NTA-degrading enzymes and genes (J. Bact. 179:1112-1116; and Appl. Environ. Microbiol. 67:688-695). The EDTA monooxygenase and NTA monooxygenase are highly homologous. EDTA monooxygenase can use both EDTA and NTA as substrates, but NTA monooxygenase can only use NTA as a substrate. Thus, we put our effort to isolate both NTA and EDTA degraders. In case, an anaerobic EDTA degrader is not immediately enriched, we will try to evolve the NTA degraders to use EDTA. Both aerobic and anaerobic enrichment cultures were set

  15. Evaluation of di-amino phenol substituted EDTA for use in radiolabelling proteins with 64Cu

    International Nuclear Information System (INIS)

    Schmidt, P.F.; Smith, S.V.; DiBartolo, N.M.

    1996-01-01

    This study involves a high yielding synthesis of a novel di-amino-phenol substituted EDTA (DAHA-EDTA) ligand and its radiolabelling chemistry with 64 Cu produced at the National Medical Cyclotron (NMC). High activity levels (up to 59.2 GBq EOB) of 64 Cu is co-produced during the production of 67 Ga from enriched 68 Zn. Waste eluent from the NMC 67 Ga production was evaporated to dryness and found to contain by products such as 57 Ni, 57 Co, 64 Cu, 67 Cu and 55 Co. A new method involving low acid concentration aqueous/organic mixtures with an anion exchange (AG 1-X8, BioRad) have been used to isolate the carrier-free 64 Cu. The specific activity of the 64 Cu (5 x 10 14 Bq/g) was found to be higher than that produce by Australian radioisotopes (ARI). The synthesis of the ligand involves the refluxing of EDTA anhydride in the presence of 4-nitro-2-amino-phenol in acetonitrile to produce the di-nitro derivative (DNHA- EDTA) in > 95% yield. The DNHA-EDTA is then reduced in the presence of activated palladium charcoal with sodium borohydride under an inert atmosphere at room temperature. The reaction mixture was acidified and the catalyst removed to obtain the final product, DAHA-EDTA. Labelling of proteins (B72.3, DD-3B6/22 and streptavidin) has been achieved with the DAHA-EDTA ligand. The reaction mixture is left to incubate for 1 h at 37 deg C and radiolabelled protein is then isolated using size exclusion chromatography

  16. 21 CFR 172.135 - Disodium EDTA.

    Science.gov (United States)

    2010-04-01

    ..., other than black-eyed peas) 165 Promote color retention. Mayonnaise 75 Preservative. Ready-to-eat cereal... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Disodium EDTA. 172.135 Section 172.135 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN...

  17. Rate constants for the reaction of e-aq with EDTA and some metal EDTA-complexes

    International Nuclear Information System (INIS)

    Buitenhuis, R.; Bakker, C.M.N.; Stock, F.R.; Louwrier, P.W.F.

    1977-01-01

    The rate constants for the reaction e - aq + EDTA were measured as a function of the pH by the pulse-radiolysis technique. Between pH = 6and pH = 10 this rate constant can be represented by the equation k = 4.7 x 10 6 x (fraction of HEDTA 3- )+1.0 x 10 8 x (fraction H 2 EDTA 2 -)M -1 s -1 . Also the rate constants for reactions of e - aq with the following metal-EDTA complexes were measured: CuEDTA 2- , HgEDTA 2- , CoEDTA 2- , InEDTA - , NiEDTA 2- , GaEDTA - , MnEDTA 2- , ZnEDTA 2- , CdEDTA 2- , PbEDTA 2- . Ionic strength variation indicates that the reacting ions are not hydrolized to an appreciable amount at pH = 11.5. It is found that some of the products show light absorption in the region between 300 and 400 nm. (orig.) [de

  18. Bicarbonate catalysis of exchange synthesis of [51Cr]Cr(III)-EDTA and other chromium complexes

    International Nuclear Information System (INIS)

    Aronson, F.L.; Strashun, A.M.; Lopez, C.; Steigman, J.

    1993-01-01

    Exchange syntheses of trivalent chromium complexes often require heating, thus limiting tagging of heat-sensitive biological compounds with 51 Cr. Bicarbonate at pH 6, accelerates the formation of mM Cr-EDTA. Accordingly, room temperature catalysis with [ 51 Cr]Cr(III) at 10 -7 -10 -8 M was investigated. Complexes were successfully formed with EDTA and iminodiacetic acid (electrophoretic analysis) and acetylacetone and tropolone (analyzed by chloroform extraction). The formation of these complexes normally requires extensive heating. (author)

  19. Protective effect by EDTA in radiation inactivation of enzymes

    Energy Technology Data Exchange (ETDEWEB)

    Kumakura, M; Kaetsu, I

    1985-11-05

    Protective effect by EDTA in radiation inactivation of enzymes such as glucoamylase, cellulase, and urease was studied. A remarkable protective effect by EDTA was observed and had a maximum at certain EDTA concentration. The protective effect was compared with other protective agents in the irradiation of urease, in which the protective ability of EDTA was greater than those of sulfhydryl compounds such as cysteine. (author).

  20. Biodegradation of PuEDTA and Impacts on Pu Mobility

    International Nuclear Information System (INIS)

    Bolton, H. Jr.; Rai, D.; Xun, L.

    2004-01-01

    The contamination of many DOE sites by Pu presents a long-term problem because of its long half-life (240,000 yrs) and the low drinking water standard ( -12 M). EDTA was co-disposed with radionuclides (e.g., Pu, 60 Co), formed strong complexes, and enhanced radionuclide transport at several DOE sites. Biodegradation of EDTA should decrease Pu mobility. One objective of this project was to determine the biodegradation of EDTA in the presence of PuEDTA complexes. The aqueous system investigated at pH 7 (10 -4 M EDTA and 10 -6 M Pu) contained predominantly Pu(OH) 2 EDTA 2- . The EDTA was degraded at a faster rate in the presence of Pu. As the total concentration of both EDTA and PuEDTA decreased (i.e., 10 -5 M EDTA and 10 -7 M PuEDTA), the presence of Pu decreased the biodegradation rate of the EDTA. It is currently unclear why the concentration of Pu directly affects the increase/decrease in rate of EDTA biodegradation. The soluble Pu concentration decreased, in agreement with thermodynamic predictions, as the EDTA was biodegraded, indicating that biodegradation of EDTA will decrease Pu mobility when the Pu is initially present as Pu(IV)EDTA. A second objective was to investigate how the presence of competing metals, commonly encountered in geologic media, will influence the speciation and biodegradation of Pu(IV)-EDTA. Studies on the solubilities of Fe(OH) 3 (s) and of Fe(OH) 3 (s) plus PuO 2 (am) in the presence of EDTA and as a function of pH showed that Fe(III) out competes the Pu(IV) for the EDTA complex, thereby showing that Pu(IV) will not form stable complexes with EDTA for enhanced transport of Pu in Fe(III) dominated subsurface systems. A third objective is to investigate the genes and enzymes involved in EDTA biodegradation. BNC1 can use EDTA and another synthetic chelating agent nitrilotriacetate (NTA) as sole carbon and nitrogen sources. The same catabolic enzymes are responsible for both EDTA and NTA degradation except that additional enzymes are

  1. The influence of EDTA application on the interactions of cadmium, zinc, and lead and their uptake of rainbow pink (Dianthus chinensis).

    Science.gov (United States)

    Lai, Hung-Yu; Chen, Zueng-Sang

    2006-10-11

    Soil used in this study was artificially contaminated with Cd, Zn, Pb, or applied in combinations (Cd-Zn, Cd-Pb, Zn-Pb, or Cd-Zn-Pb) to study the interactions of metals in soil contaminated with multiple metals. After planting rainbow pink (Dianthus chinensis) in these soils for 21 days, three different concentrations of ethylenedinitrilotetraacetic acid (EDTA) solutions were added to study the effect of applying EDTA on the interactions among these metals. The concentrations of Cd, Zn, and Pb in the soil solutions of different metals-treated soils increased significantly after applying 5 mmol EDTA kg(-1) soil (p<0.05). The potential of groundwater contamination will increase after applying EDTA and it is not recommended to be in situ used or have to use very carefully. The existence of Pb in the Cd-contaminated soil enhanced the uptake of Cd in rainbow pink in the treatments of control and 2 mmol EDTA kg(-1) soil. Cadmium inhibited the concentration of Zn without applying EDTA. However, whether the application of EDTA or not and the applied EDTA concentration had the greatest effect on the uptake of Pb when compared to Cd and Zn. After applying 5 mmol EDTA kg(-1) soil, Cd or Zn in the Pb-contaminated soil inhibited the uptake of Pb in rainbow pink, but there were no effect in other treatments.

  2. 21 CFR 73.2120 - Disodium EDTA-copper.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Disodium EDTA-copper. 73.2120 Section 73.2120 Food... COLOR ADDITIVES EXEMPT FROM CERTIFICATION Cosmetics § 73.2120 Disodium EDTA-copper. (a) Identity. The color additive disodium EDTA-copper is disodium [[N,N′- 1,2- ethanediylbis[N - (carboxymethyl) glycinato...

  3. Redistribution of fractions of zinc, cadmium, nickel, copper, and lead in contaminated calcareous soils treated with EDTA.

    Science.gov (United States)

    Jalali, Mohsen; Khanlari, Zahra V

    2007-11-01

    Effect of ethylene diamine tetraacetic acid (EDTA) on the fractionation of zinc (Zn), cadmium (Cd), nickel (Ni), copper (Cu), and lead (Pb) in contaminated calcareous soils was investigated. Soil samples containing variable levels of contamination, from 105.9 to 5803 mg/kg Zn, from 2.2 to 1361 mg/kg Cd, from 31 to 64.0 mg/kg Ni, from 24 to 84 mg/kg Cu, and from 109 to 24,850 mg/kg Pb, were subjected to EDTA treatment at different dosages of 0, 1.0, and 2.0 g/kg. Metals in the incubated soils were fractionated after 5 months by a sequential extraction procedure, in which the metal fractions were experimentally defined as exchangeable (EXCH), carbonate (CARB), Mn oxide (MNO), Fe oxide (FEO), organic matter (OM), and residual (RES) fractions. In contaminated soils without EDTA addition, Zn, Ni, Cu, and Pb were predominately present in the RES fraction, up to 60.0%, 32.3%, 41.1%, and 36.8%, respectively. In general, with the EDTA addition, the EXCH and CARB fractions of these metals increased dramatically while the OM fraction decreased. The Zn, Ni, Cu, and Pb were distributed mostly in RES, OM, FEO, and CARB fractions in contaminated soils, but Cd was found predominately in the CARB, MNO, and RES fractions. The OM fraction decreased with increasing amounts of EDTA. In the contaminated soils, EDTA removed some Pb, Zn, Cu, and Ni from MNO, FEO, and OM fractions and redistributed them into CARB and EXCH fractions. Based on the relative percent in the EXCH and CARB fractions, the order of solubility was Cd > Pb > Ni > Cu > Zn for contaminated soils, before adding of EDTA, and after adding of EDTA, the order of solubility was Pb > Cd > Zn > Ni > Cu. The risk of groundwater contamination will increase after applying EDTA and it needed to be used very carefully.

  4. Metal Recovery and Preconcentration by Edta and Dtpa Modified Silica Surfaces

    Directory of Open Access Journals (Sweden)

    Eveliina Repo

    2017-03-01

    Full Text Available This study focuses on the adsorption and preconcentration of various metals by silica gel surfaces modified with aminopolycarboxylic acids namely ethylenediaminetetraacetic acid or diethylenetriamine-pentaacetic acid. The adsorption performance of the studied materials was determined in mixed metal solutions and the adsorption isotherm studies were conducted for cobalt, nickel, cadmium, and lead. The results were modeled using various theoretical isotherm equations, which suggested that two different adsorption sites were involved in metal removal although lead showed clearly different adsorption behavior attributed to its lowest hydration tendency. Efficient regeneration of the adsorbents and preconcentration of metals was conducted with nitric acid. Results indicated that the metals under study could be analyzed rather accurately after preconcentration from both pure, saline and ground water samples.

  5. Role of CA-EDTA on the Synthesizing Process of Cerate-Zirconate Ceramics Electrolyte

    Directory of Open Access Journals (Sweden)

    Nur Athirah Abdullah

    2013-01-01

    Full Text Available The role of a combination between citric acid (CA and ethylenediaminetetra acetic acid (EDTA as chelating agents in preparation of BaCe0.54Zr0.36Y0.1O2.95 powder by a modified sol-gel method is reported. The precursor solutions were prepared from metal nitrate salts (M+, chelating agents (C, and ethylene glycol (EG at molar ratio of M+ : C : EG = 3 : 2 : 3. Chemical and phase transformation of samples during thermal decomposition were analyzed by thermogravimetric analysis (TGA and Fourier transform infrared (FTIR spectroscopy. TGA results show that the sample prepared by a combination of CA-EDTA exhibited the lowest thermal decomposition temperature, Ttd since there was no significant weight loss after 750°C. After calcined at 1100°C, the carbonates residue remained in the samples as proven by FTIR results. It was found that the used combination of CA-EDTA acts as a better combustion reagent to increase the reaction rate and influence the thermal decomposition behaviour compared to a single citric acid and EDTA, respectively. Apparently, calcination temperatures above 1100°C are needed to produce a pure perovskitic BaCe0.54Zr0.36Y0.1O2.95.

  6. Properties and reactivities of pentadentate ethylenediaminetetraacetate complexes of ruthenium(III) and -(II)

    International Nuclear Information System (INIS)

    Matsubara, T.; Creutz, C.

    1979-01-01

    The reaction of the pentadentate edta complex of ruthenium(III) with various entering ligands L has been studied as a function of pH, temperature, and L at 0.2 M ionic strength in aqueous solution. Over the pH range 0.8 to 8.5, the species Ru/sup III/(edta)H 2 O - is the sole form reactive in the substitution processes. Both forward (k 1 ) and reverse (k/sub -1/) rate constants Ru(edta)H 2 O - + L in equilibrium (k 1 ,k/sub -1/) Ru(edta)L - + H 2 O have been determined, as well as activation parameters for the complexation (k 1 ) process (L, k 1 in M -1 s -1 (25 0 C), k/sub -1/ in s -1 (25 0 C), ΔH 1 + in kcal mol -1 , ΔS 1 + in cal deg -1 mol -1 ): acetonitrile, 30 +- 7, 3.2 +- 0.2, 8.3 +- 0.5, -24 +- 4; thiocyanate, 270 +- 20, 0.5 +- 0.1, 8.9 +- 0.5, -18 +- 3; pyridine, 6300 +- 500, 0.061 +- 0.002; isonicotinamide, 8300 +- 600, 0.7 +- 0.2, 6.6 +- 0.5, -19 +- 3; pyrazine, 20,000 +- 1000, 2.0 +- 0.5, 5.7 +- 0.5, -20 +- 3. Forward rate constants for the analogous substitution reaction for Ru/sup II/(edta) H 2 O 2- (25 0 C, 0.2 M ionic strength) are as follows: thiocyanate, 2.7 +- 0.2 M -1 s -1 ; acetonitrile, 13 +- 1 M -1 s -1 ; isonicotinamide, 30 +- 15 M -1 s -1 . The substitution reactions of Ru/sup III/(edta)H 2 O - proceed by an associative mechanism, while those of Ru/sup II/(edta)H 2 O 2 - appear to be dissociative in nature. Spectra and reduction potentials for the Ru(edta)L/sup -/2-/ complexes are reported. 4 figures, 11 tables

  7. Investigation of cerium (3) complexing with ethylenediaminediacetate and ethylenediaminetetraacetate by spectrophotometric method

    International Nuclear Information System (INIS)

    Kostromina, N.A.; Kholodnaya, G.S.; Tananaeva, N.N.; Beloshitskij, N.V.; Kirillov, A.I.

    1979-01-01

    Absorption spectra in the Ce-EDDA(E 2- ) and Ce-EDTA systems (B 4- ) are studied. Decomposition of spectra into individual gauss bands, which are related to different complexes is carried out. Formation of normal complexes of the composition 1:1 and 1:2 with EDDA and EDTA is established, their stability constants being determined: lgKsub(CeE)=7.66+-0.03; lgKsub(CeEsub(2))=4.75+-0.06; lgKsub(CeB)=16.66+-0.07. It is established that in the absorption spectra additivity of the bands is observed during complexing of the more complex composition

  8. Influence of EDTA on the electrochemical removal of mercury (II) in soil from San Joaquin, Queretaro, Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Robles, I.; Serrano, T.; Perez, J. J.; Bustos, E. [Centro de Investigacion y Desarrollo Tecnologico en Electroquimica, S. C., Parque Tecnologico Queretaro, Sanfandila, Pedro Escobedo, 76703 Queretaro (Mexico); Hernandez, G.; Solis, S. [UNAM, Campus Juriquilla, Centro de Geociencias, Boulevard Juriquilla 3001, 76230 Queretaro (Mexico); Garcia, R. [UNAM, Centro de Ciencias de la Atmosfera, Ciudad Universitaria, 04510 Mexico D. F. (Mexico); Pi, T., E-mail: ebustos@cideteq.mx [UNAM, Instituto de Geologia, Ciudad Universitaria, 04510 Mexico D. F. (Mexico)

    2014-07-01

    The removal of mercury from soil and Ca-bentonite was performed using electrochemical treatment adding ethylendiamine-tetra acetic acid (EDTA) as a complexing agent to improve the electrochemical removal of Hg (II) in soil from San Joaquin, Queretaro, Mexico. During the electrokinetic treatment in the presence of 0.1 M EDTA, most of Hg (II) migrates toward the anode obtaining the highest removal efficiencies close to 70% in bentonite after 9 h. Using 0.1 M HCl only 65% efficiency was attained after 13 h in the cathodic side. EDTA formed a negatively charged stable complex that migrates to the cathode by the application of the electrokinetic treatment across Hg - EDTA synthesized complex. Finally, the predominant crystallographic structures of the samples were examined using X-ray diffraction. (Author)

  9. Influence of EDTA on the electrochemical removal of mercury (II) in soil from San Joaquin, Queretaro, Mexico

    International Nuclear Information System (INIS)

    Robles, I.; Serrano, T.; Perez, J. J.; Bustos, E.; Hernandez, G.; Solis, S.; Garcia, R.; Pi, T.

    2014-01-01

    The removal of mercury from soil and Ca-bentonite was performed using electrochemical treatment adding ethylendiamine-tetra acetic acid (EDTA) as a complexing agent to improve the electrochemical removal of Hg (II) in soil from San Joaquin, Queretaro, Mexico. During the electrokinetic treatment in the presence of 0.1 M EDTA, most of Hg (II) migrates toward the anode obtaining the highest removal efficiencies close to 70% in bentonite after 9 h. Using 0.1 M HCl only 65% efficiency was attained after 13 h in the cathodic side. EDTA formed a negatively charged stable complex that migrates to the cathode by the application of the electrokinetic treatment across Hg - EDTA synthesized complex. Finally, the predominant crystallographic structures of the samples were examined using X-ray diffraction. (Author)

  10. The study of operating variables in soil washing with EDTA

    International Nuclear Information System (INIS)

    Zou Zeli; Qiu Rongliang; Zhang Weihua; Dong Hanying; Zhao Zhihao; Zhang Tao; Wei Xiange; Cai Xinde

    2009-01-01

    This study discusses the operating variables for removal of metals from soils using EDTA, including the type of EDTA, reaction time, solution pH, dose, temperature, agitation, ultrasound and number of extractions. For As, Cd, Cu, Pb and Zn, the removal efficiency order was: H 4 -EDTA > Na 2 EDTA > (NH 4 ) 2 EDTA. At low EDTA concentrations the removal increased progressively with increasing dose while above 0.4 mmol/g only small increases in extraction efficiency were observed. EDTA induced a two-step process including a rapid desorption within the first hour, and a gradual release in the following hours. The extraction efficiency of metals decreased with increasing pH in the range of 2-10. Consecutive extractions using low concentrations were more effective than a single extraction with concentrated EDTA if the same dose of EDTA was used. - Consecutive extractions using low concentrations are more effective than a single extraction with concentrated EDTA if the same dose of EDTA is used

  11. Chromium 51-ethylenediaminetetraacetate test: a useful test in the assessment of inflammatory bowel disease

    International Nuclear Information System (INIS)

    O'Morain, C.A.; Abelow, A.C.; Chervu, L.R.; Fleischner, G.M.; Das, K.M.

    1986-01-01

    We evaluated the usefulness of urinary excretion values in assessing mucosal damage in inflammatory bowel disease after administration of chromium 51-labeled EDTA either orally or rectally. In the oral study, 19 controls, 18 patients with Crohn's disease, and 13 patients with ulcerative colitis were given 100 microCi 51 Cr-EDTA by mouth. The amount of 51 Cr-EDTA in a 24-hour urine collection was expressed as a percentage of the ingested dose. The patients with Crohn's disease of the small bowel excreted 6.3% +/- 4.3%, which was significantly (P less than 0.001) higher than the percentage in patients with ulcerative colitis (1.7% +/- 1.1%) and controls (1.4% +/- 0.6%). In the enema study, 19 patients with ulcerative colitis, two with Crohn's disease, two with radiation colitis, and four controls (spastic colitis, lactose intolerance) were given 100 microCi 51 Cr-EDTA by retention enema. The patients with active colonic inflammation excreted 8.4% +/- 3.9% of the dose given by enema, which was significantly (P less than 0.01) higher than in other controls (1.9% +/- 0.91%) or patients with inactive colitis (2.2% +/- 1.9%). The 51 Cr-EDTA excretion test is a safe, inexpensive test useful in evaluating patients with inflammatory bowel disease. It can be given orally to screen patients with abdominal complaints who are suspected of having Crohn's disease involving the small intestine, and when given by enema it provides additional objective assessment of idiopathic ulcerative colitis or proctitis

  12. Chromium 51-ethylenediaminetetraacetate test: a useful test in the assessment of inflammatory bowel disease

    Energy Technology Data Exchange (ETDEWEB)

    O' Morain, C.A.; Abelow, A.C.; Chervu, L.R.; Fleischner, G.M.; Das, K.M.

    1986-11-01

    We evaluated the usefulness of urinary excretion values in assessing mucosal damage in inflammatory bowel disease after administration of chromium 51-labeled EDTA either orally or rectally. In the oral study, 19 controls, 18 patients with Crohn's disease, and 13 patients with ulcerative colitis were given 100 microCi /sup 51/Cr-EDTA by mouth. The amount of /sup 51/Cr-EDTA in a 24-hour urine collection was expressed as a percentage of the ingested dose. The patients with Crohn's disease of the small bowel excreted 6.3% +/- 4.3%, which was significantly (P less than 0.001) higher than the percentage in patients with ulcerative colitis (1.7% +/- 1.1%) and controls (1.4% +/- 0.6%). In the enema study, 19 patients with ulcerative colitis, two with Crohn's disease, two with radiation colitis, and four controls (spastic colitis, lactose intolerance) were given 100 microCi /sup 51/Cr-EDTA by retention enema. The patients with active colonic inflammation excreted 8.4% +/- 3.9% of the dose given by enema, which was significantly (P less than 0.01) higher than in other controls (1.9% +/- 0.91%) or patients with inactive colitis (2.2% +/- 1.9%). The /sup 51/Cr-EDTA excretion test is a safe, inexpensive test useful in evaluating patients with inflammatory bowel disease. It can be given orally to screen patients with abdominal complaints who are suspected of having Crohn's disease involving the small intestine, and when given by enema it provides additional objective assessment of idiopathic ulcerative colitis or proctitis.

  13. Treatment of EDTA contained reactor coolant using water dielectric barrier discharge plasma

    International Nuclear Information System (INIS)

    Song, Sang Heon; Kwon, Daniel; Kim, Gon Ho

    2005-01-01

    EDTA (Ethylene Diamine Tetraacetic Acid) is used as a main absorbent for the metal ion in the secondary loop of the nuclear reactor. Dissolving the wasted EDTA with low cost, therefore, is important issue for the maintenance of the nuclear power reactor and the protection of environment. EDTA is not easily biodegradable, furthermore these methods could make remained another pollutant as complex chemical compounds. Compared to chemical method, the physical methods, using the energetic particles and UVs, are more favorable because they dissociate the bonds of organic compounds directly without the secondary chemical reactions during the treatment. Recently, high energy electron beam, the plasma torch, and the water breakdown by high voltage pulse are applied to treatment of the waste water contained chemicals. Here consideration is narrow down to improve the interaction between the plasma and the chemical bonds of EDTA because the energetic particles; activated radicals, and UVs, are abundant in plasmas. The new method adapted of the water DBD (dielectric barrier discharge) which plasma generates directly on the top of the water contained EDTA is proposed. The application of DBD plasmas has been extended for cleaning the organic compounds from the contaminated surface and also for removing volatile organic chemicals (VOC) such as NO x and SO x from the exhausted gases. Here, the water DBD reactor (SEMTECH, SD-DWG-04-1) is consisted that the one electrode is a ceramic insulator and another one is the water itself. Interestingly, the one electrode, the water, is not the solid dielectric electrode. In this study, therefore, the characteristics with driving frequency are considered and the feasibility of this new method for the DBD treatment of EDTA contained water is demonstrated

  14. Application of EDTA-functionalized bamboo activated carbon (BAC) for Pb(II) and Cu(II) removal from aqueous solutions

    Science.gov (United States)

    Lv, Dan; Liu, Yu; Zhou, Jiasheng; Yang, Kunlun; Lou, Zimo; Baig, Shams Ali; Xu, Xinhua

    2018-01-01

    In this study, a novel bamboo activated carbon (BAC) with ethylene diamine tetraacetic acid (EDTA) functionality was prepared by direct grafting in the presence of tetraethyl orthosilicate (TEOS) as a crosslinking agent. The BAC@SiO2-EDTA was characterized by SEM, TEM, TGA, FTIR, XPS and its adsorption property for removal of Pb(II) and Cu(II) under various experimental conditions was also investigated. The characterization results reflected that EDTA was successfully assembled on the surface of the BAC and average pore size increased from 4.10 to 4.83 nm as BAC grafted with EDTA. Adsorption data fitted very well in Langmuir isotherm model and pseudo-second-order kinetic model. As compared with the raw BAC, the maximum adsorption capacities of BAC@SiO2-EDTA for the Pb(II) and Cu(II) increased from 45.45 to 123.45 mg g-1 and from 6.85 to 42.19 mg g-1, since the existence of EDTA on modified BAC promoted the formation of chemical complex. The removal of heavy metal ions mainly depended on the complexation with EDTA and the electrostatic attractions with negatively charged surface of BAC@SiO2-EDTA. The adsorption of Pb(II)/Cu(II) on the BAC@SiO2-EDTA was pH dependent and pH 5-6 was considered an optimum. However, lower temperature favored the adsorption and the maximum adsorption was recorded at 20 °C. In addition, BAC@SiO2-EDTA had an excellent reusability with about 40% decline in the adsorption capacity for Pb(II) after fifth reuse. Insignificant influences of co-existing cations and natural organic matter (NOM) were found on the adsorption of Pb(II) and Cu(II). All the results demonstrate that BAC@SiO2-EDTA is a potential adsorbent for metal ions in wastewater.

  15. HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design

    International Nuclear Information System (INIS)

    Oliveira, Karina B. de; Oliveira, Bras H. de

    2013-01-01

    Sage (Salvia officinalis) contains high amounts of the biologically active rosmarinic acid (RA) and other polyphenolic compounds. RA is easily oxidized, and may undergo degradation during sample preparation for analysis. The objective of this work was to develop and validate an analytical procedure for determination of RA in sage, using factorial design of experiments for optimizing sample preparation. The statistically significant variables for improving RA extraction yield were determined initially and then used in the optimization step, using central composite design (CCD). The analytical method was then fully validated, and used for the analysis of commercial samples of sage. The optimized procedure involved extraction with aqueous methanol (40%) containing an antioxidant mixture (ascorbic acid and ethylenediaminetetraacetic acid (EDTA)), with sonication at 45 deg C for 20 min. The samples were then injected in a system containing a C 18 column, using methanol (A) and 0.1% phosphoric acid in water (B) in step gradient mode (45A:55B, 0-5 min; 80A:20B, 5-10 min) with flow rate of 1.0 mL min−1 and detection at 330 nm. Using this conditions, RA concentrations were 50% higher when compared to extractions without antioxidants (98.94 ± 1.07% recovery). Auto-oxidation of RA during sample extraction was prevented by the use of antioxidants resulting in more reliable analytical results. The method was then used for the analysis of commercial samples of sage. (author)

  16. HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina B. de [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Dept. de Farmacia; Oliveira, Bras H. de, E-mail: bho@ufpr.br [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Dept. de Quimica

    2013-01-15

    Sage (Salvia officinalis) contains high amounts of the biologically active rosmarinic acid (RA) and other polyphenolic compounds. RA is easily oxidized, and may undergo degradation during sample preparation for analysis. The objective of this work was to develop and validate an analytical procedure for determination of RA in sage, using factorial design of experiments for optimizing sample preparation. The statistically significant variables for improving RA extraction yield were determined initially and then used in the optimization step, using central composite design (CCD). The analytical method was then fully validated, and used for the analysis of commercial samples of sage. The optimized procedure involved extraction with aqueous methanol (40%) containing an antioxidant mixture (ascorbic acid and ethylenediaminetetraacetic acid (EDTA)), with sonication at 45 deg C for 20 min. The samples were then injected in a system containing a C{sub 18} column, using methanol (A) and 0.1% phosphoric acid in water (B) in step gradient mode (45A:55B, 0-5 min; 80A:20B, 5-10 min) with flow rate of 1.0 mL min-1 and detection at 330 nm. Using this conditions, RA concentrations were 50% higher when compared to extractions without antioxidants (98.94 {+-} 1.07% recovery). Auto-oxidation of RA during sample extraction was prevented by the use of antioxidants resulting in more reliable analytical results. The method was then used for the analysis of commercial samples of sage. (author)

  17. The status of phosphorus in Thai soils and P evaluation using EDTA-NaF extraction method

    Directory of Open Access Journals (Sweden)

    Toru Matoh

    2003-07-01

    Full Text Available Although the available P extracted by Bray II method in tropical soil is low, most of tropical plants can grow well. The objective of this study was to study P status and to evaluate the available P extracted by EDTA-NaF method. Top soil and sub soil of 10 dominant soil series in Thailand were analyzed for some chemical properties and characterization of the forms of phosphorus using EDTA-NaF extraction and successive phosphorus extraction by the modified Sekiya method. The soil total P concentration was 38-1137 mg P2O5 kg-1. The available Bray II-P was very low to high (1-76 mg P2O5 kg-1, and it approximated 0.17-12% of the total P. Iron and aluminum phosphates were the main fraction of inorganic P in acid soil, whereas Ca phosphates were in calcareous soils. Organic P content accounted for 33-67% and most of them were bound with Fe and Al in acid soils and Ca in calcareous soils. P extracted by EDTA-NaF reagent was obviously larger than that of Bray II reagent. The EDTA-NaF extracted P [high molecular weight organic P (HMWP+ inorganic P (EDTA ext Pi] was 7-46% and 1-6% of total P in acid soils and calcareous soils respectively. The EDTA ext Pi tended to be larger than HMWP except in Tk soil. The total amount of extracted P correlated well with Al-Pi and Fe-Pi which were the main fraction of inorganic P. It also correlated with HMWP, but HMWP did not correlate with organic P determine by ignition method and Ca-Po, Fe-Po and Al-Po. The EDTA-NaF method may be suitable for P evaluation in the soils which have high amounts of Fe-Pi, Al -Pi and organic P widely distributed in Thailand.

  18. Effects of EDTA on the electronic properties of passive film formed on Fe-20Cr in pH 8.5 buffer solution

    International Nuclear Information System (INIS)

    Cho, Eun Ae; Kwon, Hyuk Sang; Beranrd, Frederic

    2003-01-01

    The electronic properties of the passive film formed on Fe-20Cr ferritic stainless steel in pH 8.5 buffer solution containing 0.05 M EDTA (ethylene diammine tetraacetic acid) were examined by the photocurrent measurements and Mott-Schottky analysis for the film. XPS depth profile for the film demonstrated that Cr content in the outermost layer of the passive film was higher in the solution with EDTA than that in the solution without EDTA, due to selective dissolution of Fe by EDTA. In the solution with EDTA, the passive film showed characteristics of an amorphous or highly disordered n-type semiconductor. The band gap energies of the passive film are estimated to be ∼ 3.0 eV, irrespective of film formation potential from 0 to 700 mV SCE and of presence of EDTA. However, the donor density of the passive film formed in the solution with EDTA is much higher than that formed in the solution without EDTA, due to an increase in oxygen vacancy resulted from the dissolution of Fe-oxide in the outermost layer of the passive film. These results support the proposed model that the passive film formed on Fe-20Cr in pH 8.5 buffer solution mainly consists of Cr-substituted γ-Fe 2 O 3

  19. Kinetics of ethylenediaminetetraacetate exchange of americium (3) with copper (2) in aqueous solution

    International Nuclear Information System (INIS)

    Nikitenko, S.I.; Martynenko, L.I.; Pechurova, N.I.

    1985-01-01

    By the method of spectrophotometry exchange kinetics in the AmA - -Cu 2+ , where A 4- -ethylenediaminetetraacetate, is studied. The values of exchange rate constants and thermodynamic activation parameters have been found. It is shown that exchange of central ions is rialized according to the dissociative mechanism with formation of intermediate protonated complexes and according to the associative mechanism with formation of binuclear intermediates. The exchange mechanisms identity for AmA - and LnA - , where Ln 3+ -RE cations of cerium subgroup is proved. It is assumed that values of activation entropy in exchange processes are determined by the radius of the leaving cation and of activation enthalpy - by the peculiarities of the electronic structure

  20. Spectroscopic investigation of solutions temperature effect on structure of americium(3) complexes with ethylenediaminetetraacetate and hydroxyethylethylenediaminetriacetate

    International Nuclear Information System (INIS)

    Nikitenko, S.I.; Martynenko, L.I.; Pechurova, N.I.

    1985-01-01

    Temperature dependence of absorption band intensity in spectra of solutions AmA - and AmX, where A 4- - ethylenediaminetetraacetate, X 3- - hydroxyethyl ethylenediaminetriacetate, has been studied spectrophotometrically. It is detected, that with the increase in solution temperature in the range 25-86 deg C a redistribution of absorption band intensities in AmA - X and AmX spectra is observed. Spectrum of Am 3+ never varies in the studied temperature range. It is assumed, that the observed phenomenon is explained by the change in the structure of Am 3+ complexonates during solution heating. Values of ΔH of the processes of internal coordination sphere rebuilding are calculated: 3.3+-0.9 (AmA - ), 2.2+-0.8 (AmX) kJ/mol

  1. Reactivity of two hydrate forms of europium(3) ethylenediaminetetraacetate in the processes of central ion substitution

    International Nuclear Information System (INIS)

    Nikitenko, S.I.; Martynenko, L.I.; Pechurova, N.I.

    1985-01-01

    Kinetics of central ion substitution in the system EuA - -Cu 2+ , where A 4- -ethylenediaminetetraacetate (pH 6.0, t=25-55 deg C), has been studied spectrophotometrically. It is shown, that the form EuA(H 2 O) 3 - is more reactive as compared with EuA(H 2 O) 2 - . It is found, that equilibrium constant of the process EuA(H 2 O) 3 - reversible EuA(H 2 O) 2 - (1) equals 1 at t=36.2 deg C. The values ΔH and ΔS of the process (1), constituting 13.0+-3.2 kJ/mol and 41.8+-5.5 J/molxdeg respectively, are calculated

  2. The effect of Emdogain and 24% EDTA root conditioning on periodontal healing of replanted dog's teeth.

    Science.gov (United States)

    Guzmán-Martínez, Nayelli; Silva-Herzog, Flores Daniel; Méndez, González Verónica; Martín-Pérez, Silvia; Cerda-Cristerna, Bernardino Isaac; Cohenca, Nestor

    2009-02-01

    Controversies still exist as for the regenerative role of enamel matrix derivatives and the need for removal of the periodontal ligament in replanted teeth. The purpose of this study was to evaluate the effect of Emdogain and 24% ethylenediamine tetraacetic acid (EDTA) root conditioning on periodontal healing of replanted dog's teeth. Teeth were extracted, endodontically treated and preconditioned as follows: group 1, Emdogain; group 2, Emdogain + EDTA and group 3, EDTA. Teeth were replanted after 30 min extraoral time, splinted for 15 days and animals sacrificed after 8 weeks of observation. Histological evaluation was performed using hematoxylin/eosin and Masson trichrome and results scored based on previously reported criteria for histological evaluation. Replacement root resorption was histologically diagnosed in all groups except in the negative control. A parametric analysis showed no statistically significant differences between experimental groups. Root preconditioning with Emdogain alone or in combination with 24% EDTA showed no evidence of regeneration of collagen fibers and consequently did not prevent the development of replacement root resorption on replanted dog's teeth.

  3. DNAPL remediation with in situ chemical oxidation using potassium permanganate - Part I. Mineralogy of Mn oxide and its dissolution in organic acids

    Science.gov (United States)

    Li, X. David; Schwartz, Franklin W.

    2004-01-01

    Previous studies on in situ chemical oxidation of trichloroethylene (TCE) with potassium permanganate indicated that the solid reaction product, Mn oxide, could reduce the permeability of the porous medium and impact the success of dense non-aqueous phase liquid (DNAPL) removal. In order to address the issue of permeability reduction caused by precipitation, this study investigated the mineralogy of Mn oxides and the possibilities of removing the solid precipitates by dissolution. The solid reaction product from the oxidation of TCE by permanganate is semi-amorphous potassium-rich birnessite, which has a layered mineral structure with an interlayer spacing of 7.3 Å. The chemical formula is K 0.854Mn 1.786O 4·1.55H 2O. It has a relatively small specific surface area at 23.6±0.82 m 2/g. Its point of zero charge (pzc) was measured as 3.7±0.4. This birnessite is a relatively active species and could participate in various reactions with existing organic and inorganic matter. The dissolution kinetics of Mn oxide was evaluated in batch experiments using solutions of citric acid, oxalic acid, and ethylenediaminetetraacetic acid (EDTA). Initial dissolution rates were determined to be 0.126 mM/m 2/h for citric acid, 1.35 mM/m 2/h for oxalic acid, and 5.176 mM/m 2/h for EDTA. These rates compare with 0.0025 mM/m 2/h for nitric acid at pH=2. Organic acids dissolve Mn oxide quickly. Reaction rates increase with acid concentration, as tested with citric acid. The dissolution mechanism likely involves proton and ligand-promoted dissolution and reductive dissolution. Citric and oxalic acid can induce ligand-promoted dissolution, while EDTA can induce ligand-promoted and reductive dissolutions. At low pH, proton-promoted dissolution seems to occur with all the acids tested, but this process is not dominant. Reductive dissolution appears to be the most effective process in dissolving the solid, followed by ligand-promoted dissolution. These experiments indicate the significant

  4. Effect of NaFeEDTA-Fortified Soy Sauce on Anemia Prevalence in China: A Systematic Review and Meta-analysis of Randomized Controlled Trials.

    Science.gov (United States)

    Huo, Jun Sheng; Yin, Ji Yong; Sun, Jing; Huang, Jian; Lu, Zhen Xin; Regina, Moench-Pfanner; Chen, Jun Shi; Chen, Chun Ming

    2015-11-01

    To assess the effect of sodium iron ethylenediaminetetraacetate (NaFeEDTA)-fortified soy sauce on anemia prevalence in the Chinese population. A systematic review was performed to identify potential studies by searching the electronic databases of PubMed, Cochrane Library, WHO Library, HighWire, CNKI, and other sources. The selection criteria included randomized controlled trials that compared the efficacy of NaFeEDTA-fortified soy sauce with that of non-fortified soy sauce. Anemia rates and hemoglobin levels were the outcomes of interest. Inclusion decisions, quality assessment, and data extraction were performed by two reviewers independently. A total of 16 studies met the inclusion criteria for anemia rate analysis, of which 12 studies met the inclusion criteria for hemoglobin analysis. All included studies assessed the effect of NaFeEDTA-fortified soy sauce on anemia rates and hemoglobin concentrations. After the intervention, the hemoglobin concentration increased and anemia rates decreased significantly as compared with the non-fortified soy sauce groups. For anemia rates, data from 16 studies could be pooled, and the pooled estimate odds ratio was 0.25 (95% CI 0.19-0.35). For hemoglobin concentrations, data from 12 studies could be pooled, and the pooled weighted mean difference was 8.81 g/L (95% CI 5.96-11.67). NaFeEDTA-fortified soy sauce has a positive effect on anemia control and prevention in the at-risk population. Copyright © 2015 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.

  5. Thermometric titration of thorium with EDTA in the presence of large excess of neutral sodium salts.

    Science.gov (United States)

    Doi, K

    1980-11-01

    The thermometric titration of Th(IV) in the presence of neutral sodium salts, sulphuric acid or acetic acid with EDTA has been studied. The effect of each on the observed heat values for the titration is discussed. For sodium perchlorate media, DeltaH values of -9 and -21 kJ/mole have been estimated for the formation of the Th(IV)-EDTA chelate at mu --> 0 and mu = 0.5 (NaClO(4)), respectively. The -DeltaH values increase steadily with increase in concentration of sodium perchlorate up to at least 3M. For the titration of Th(IV) in the presence of a large excess of sodium nitrate the use of sodium iodide as a masking reagent has been examined: large amounts of Bi and Cu(II) are masked and a masking effect is observed for small amounts of Ni.

  6. Outer Sphere Adsorption of Pb(II)EDTA on Goethite

    Energy Technology Data Exchange (ETDEWEB)

    Bargar, John R

    1999-07-16

    FTIR and EXAFS spectroscopic measurements were performed on Pb(II)EDTA adsorbed on goethite as functions of pH (4-6), Pb(II)EDTA concentration (0.11 {micro}M - 72 {micro}M), and ionic strength (16 {micro}M - 0.5M). FTIR measurements show no evidence for carboxylate-Fe(III) bonding or protonation of EDTA at Pb:EDTA = 1:1. Both FTIR and EXAFS measurements suggest that EDTA acts as a hexadentate ligand, with all four of its carboxylate and both amine groups bonded to Pb(II). No evidence was observed for inner-sphere Pb(II)-goethite bonding at Pb:EDTA = 1:1. Hence, the adsorbed complexes should have composition Pb(II)EDTA{sup 2{minus}}. Since substantial uptake of PbEDTA(II){sup 2{minus}} occurred in the samples, we infer that Pb(II)EDTA{sup 2{minus}} adsorbed as outer-sphere complexes and/or as complexes that lose part of their solvation shells and hydrogen bond directly to goethite surface sites. We propose the term ''hydration-sphere'' for the latter type of complexes because they should occupy space in the primary hydration spheres of goethite surface functional groups, and to distinguish this mode of sorption from common structural definitions of inner- and outer-sphere complexes. The similarity of Pb(II) uptake isotherms to those of other divalent metal ions complexed by EDTA suggests that they too adsorb by these mechanisms. The lack of evidence for inner-sphere EDTA-Fe(III) bonding suggests that previously proposed metal-ligand - promoted dissolution mechanisms should be modified, specifically to account for the presence of outer-sphere precursor species.

  7. Protective effect of EDTA preadministration on renal ischemia

    Directory of Open Access Journals (Sweden)

    Belloni Daniela

    2006-03-01

    Full Text Available Abstract Background Chelation therapy with sodium edetate (EDTA improved renal function and slowed the progression of renal insufficiency in patients subjected to lead intoxication. This study was performed to identify the underlying mechanism of the ability of EDTA treatment to protect kidneys from damage. Methods The effects of EDTA administration were studied in a rat model of acute renal failure induced by 60 minutes ischemia followed or not by 60 minutes reperfusion. Renal ischemic damage was evaluated by histological studies and by functional studies, namely serum creatinine and blood urea nitrogen levels. Treatment with EDTA was performed 30 minutes before the induction of ischemia. Polymorphonuclear cell (PMN adhesion capability, plasmatic nitric oxide (NO levels and endothelial NO synthase (eNOS renal expression were studied as well as the EDTA protection from the TNFα-induced vascular leakage in the kidneys. Data was compared by two-way analysis of variance followed by a post hoc test. Results EDTA administration resulted in the preservation of both functional and histological parameters of rat kidneys. PMN obtained from peripheral blood of EDTA-treated ischemized rats, displayed a significant reduction in the expression of the adhesion molecule Mac-1 with respect to controls. NO was significantly increased by EDTA administration and eNOS expression was higher and more diffuse in kidneys of rats treated with EDTA than in the controls. Finally, EDTA administration was able to prevent in vivo the TNFα-induced vascular leakage in the kidneys. Conclusion This data provides evidence that EDTA treatment is able to protect rat kidneys from ischemic damage possibly through the stimulation of NO production.

  8. The effect of vacuum packaging, EDTA, oregano and thyme oils on the microbiological quality of chicken's breast.

    Science.gov (United States)

    Pavelková, Adriana; Kačániová, Miroslava; Horská, Elena; Rovná, Katarína; Hleba, Lukáš; Petrová, Jana

    2014-10-01

    The effect of ethylenediaminetetraacetate (EDTA), oregano (Origanum vulgare) and thyme (Thymus vulgaris) oils, on the chicken breast fillets was examined in this study. The chicken breast fillets were stored under vacuum packaging (VP), at 4 ± 0.5 °C for a period of 18 days. There were used the following treatments of chicken breast fillets: Air-packaged (AC, control samples), vacuum-packaged (VPC, control samples), VP with EDTA solution 1.50% w/w (VPEC, control samples), VP with oregano oil 0.20% v/w (VP + O) and VP with thyme oil 0.20% v/w, (VP + T). The quality assessment for vacuum packaging of the product in accordance with the terms above and EDTA treatment, oregano and thyme oil was established by microbiological analyzes. The microbiological properties as the total viable counts on Plate Count Agar, after incubation for 2 days at 37 °C and coliform bacteria on Violet Red Bile Glucose agar incubated at 37 °C for 24 h, lactobacilli on Rogosa and Sharpe agar after incubation 48-78 h at 37 °C in an aerobic atmosphere supplemented with carbon dioxide (5% CO2) and Pseudomonas aeruginosa on Pseudomonas Isolation agar (PIA, Oxoid, UK) after incubation at 48 h at 35 °C were monitored. The using of oregano, thyme oil and EDTA with combination of vacuum packaging has significant effects to reduction of all followed groups of microorganisms compared with control group without vacuum packaging and untreated control group. The natural preservatives can be used as alternatives to chemical additives which could extend the meat and meat products shelf life. The knowledge about them can have an important economic feedback by reducing losses attributed to spoilage and by allowing the products to reach distant and new markets. This study shows how using of natural antimicrobials can extend the shelf-life of the meat product. Copyright © 2013 Elsevier Ltd. All rights reserved.

  9. Kinetics of electrophilic exchange of lanthanide(3) and copper(2) ions in aqueous solutions of ethylenediaminetetraacetates. [Rare earths: La,Pr,Nd,Eu,Gd,Tb,Er,Yb

    Energy Technology Data Exchange (ETDEWEB)

    Nikitenko, S.I.; Martynenko, L.I.; Pechurova, N.I.; Spitsyn, V.I. (Moskovskij Gosudarstvennyj Univ. (USSR))

    1984-05-01

    Electrophilic exchange in the MA/sup -/ - Cu/sup 2 +/ (M/sup 3 +/=La/sup 3 +/, PUPSILON/sup 3 +/, Nd/sup 3 +/, Eu/sup 3 +/, Gd/sup 3 +/, Tb/sup 3 +/, Er/sup 3 +/, Yb/sup 3 +/, A/sup 4 -/=ethylenediaminetetraacetate) systems is studied. Copper is chosen as a constituent cation, because the stability of the CuA/sup 2 +/- complex is higher than that of the MA/sup -/ complex, besides the CuA/sup 2 -/, is characterized by a high extinction coefficient E/sub 280/=3200. Electrophilic exchange in the systems is shown to be realized through the acid dissociation mechanism and through the association mechanism. Consideration of the acid dissociation mechanism assumes that the exchange is limited by a break in the metal-oxygen bond in the intermediate protonated complex. In case of the association mechanism it is limited by a break in the metal-nitrogen bond in the intermediate binuclear complex. Rate constants of the limiting stage are calculated. Dependences of the rate constants of exchange on the stability of the complexes, the order number of the element, acidity of medium, Cu/sup 2 +/ concentration are given. Differences are shown to exist between the exchange mechanisms in the studied: systems for the REE of cerium and yttrium subgroups.

  10. HYDROTALSIT Zn-Al-EDTA SEBAGAI ADSORBEN UNTUK POLUTAN ION Pb(II DI LINGKUNGAN Zn-Al-EDTA Hydrotalcite as Adsorbent for Pb(II Ion Pollutant in The Environment

    Directory of Open Access Journals (Sweden)

    Roto Roto

    2015-07-01

    Full Text Available ABSTRAK Polusi ion Pb(II di dalam lingkungan perairan cenderung naik seiring peningkatan jumlah industri smelter dan daur ulang aki bekas. Penelitian ini bertujuan untuk menguji kemampuan hidrotalsit Zn-Al-EDTA sebagai adsorben ion Pb(II dalam air secara mendalam. Hidrotalsit Zn-Al-NO3 disintesis dengan metode kopresipitasi dan hidrotermal pada temperatur 100 °C selama 15 jam. Hidrotalsit Zn-Al-EDTA diperoleh dengan penukaran ion. Keasaman larutan, kinetika dan kapasitas adsorpsi diteliti. Hidrotalsit Zn-Al-EDTA memiliki d003 sebesar 14,52 Å sementara Zn-Al-NO3 sebesar 8,90 Å. Spektra FTIR menunjukkan keberadaan serapan gugus C=O pada bilangan gelombang 1684,77 cm-1. Kondisi optimum adsorpsi ion Pb(II terjadi pada pH 4, waktu kontak 60 menit dan kapasitas adsorpsi diperoleh 2,07 mg/g pada konsentrasi awal 10 mg/L dengan berat adsorben 0,100 g. Adsorpsi ion Pb(II oleh hidrotalsit Zn-Al-EDTA mengikuti reaksi pseudo orde dua dengan tetapan laju adsorpsi sebesar 8,90 g mmol-1min-1. Adsorpsi ion Pb(II oleh Zn-Al-EDTA terjadi karena  pembentukan khelat Pb-EDTA di dalam struktur hidrotalsit. Hasil ini diharapkan mampu memberikan kontribusi yang lebih luas di dalam pengendalian konsentrasi Pb(II di lingkungan. ABSTRACT Polution by Pb(II ion in the water environment tends to increase due the increase in the number of lead smelter and lead acid battery recycling industries. This work aims at studying in details the ability of Zn-Al-EDTA hydrotalcite as adsorbent for Pb(II ion in the environment. The Zn-Al-NO3 hydrotalcite was synthesized first by coprecipitation method followed by hydrothermal treatment at 100 °C for 15 h. The Zn-Al-EDTA hydrotalcite was later obtained by ion exchange process. The solution pH, kinetics and adsorption capacity were studied. The XRD data showed that Zn-Al-EDTA and Zn-Al-NO3 hydrotalcites have d003 of 14.52 and 8.90 Å, respectively. The FTIR spectra suggested that C=O group was observed with absorption band at 1684

  11. The influence of EDTA application on the interactions of cadmium, zinc, and lead and their uptake of rainbow pink (Dianthus chinensis)

    International Nuclear Information System (INIS)

    Lai, H.-Y.; Chen, Z.-S.

    2006-01-01

    Soil used in this study was artificially contaminated with Cd, Zn, Pb, or applied in combinations (Cd-Zn, Cd-Pb, Zn-Pb, or Cd-Zn-Pb) to study the interactions of metals in soil contaminated with multiple metals. After planting rainbow pink (Dianthus chinensis) in these soils for 21 days, three different concentrations of ethylenedinitrilotetraacetic acid (EDTA) solutions were added to study the effect of applying EDTA on the interactions among these metals. The concentrations of Cd, Zn, and Pb in the soil solutions of different metals-treated soils increased significantly after applying 5 mmol EDTA kg -1 soil (p -1 soil. Cadmium inhibited the concentration of Zn without applying EDTA. However, whether the application of EDTA or not and the applied EDTA concentration had the greatest effect on the uptake of Pb when compared to Cd and Zn. After applying 5 mmol EDTA kg -1 soil, Cd or Zn in the Pb-contaminated soil inhibited the uptake of Pb in rainbow pink, but there were no effect in other treatments

  12. The ERA-EDTA today and tomorrow: a progress document by the ERA-EDTA Council.

    Science.gov (United States)

    Zoccali, Carmine; Arici, Mustafa; Blankestijn, Peter J; Bruchfeld, Annette; Capasso, Giovambattista; Fliser, Danilo; Fouque, Denis; Goumenos, Dimitrios; Ketteler, Markus; Malyszko, Jolanta; Massy, Ziad; Rychlík, Ivan; Spasovski, Goce

    2018-05-23

    Scientific societies are increasingly seen as central to the advancement of information sharing and collaboration among scientists and clinical investigators for the progress of medical research and the promotion of education, professional competence, integrity and quality studies. To more effectively serve the practicing nephrologists and investigators dedicated to renal science, the Council of the European Renal Association and European Dialysis and Transplantation Association (ERA-EDTA) reorganized and integrated the various activities of the society into two branches, the Clinical Nephrology Governance branch and the Renal Science branch. New affordable initiatives to promote research, education and professional development and to advocate for the recognition of chronic kidney disease as a major public health issue at the European level will be put in place and/or potentiated in the new organizational frame. Educational initiatives will be espoused to Continuous Professional Development and, starting from 2019, 14 Education & Continuous Professional Development courses will be held covering the full range of knowledge areas of modern nephrology. Consolidation and development is the short- and medium-term mantra of the ERA-EDTA. The society has a rich portfolio of successful activities and brilliant, creative scientists among its members. Integrating the various activities of the ERA-EDTA and treasuring the expertise and wisdom of its most accomplished members will facilitate collaborative research, education and its public impact at large.

  13. Effects of calcium binding and of EDTA and CaEDTA on the clotting of bovine fibrinogen by thrombin.

    Science.gov (United States)

    Perizzolo, K E; Sullivan, S; Waugh, D F

    1985-03-01

    Studies were carried out at pH 7.0 and gamma/2 0.15 before addition of CaCl2 or EDTA. Clotting time, tau, at 3.03 microM fibrinogen and 0.91 u/ml thrombin was determined for equilibrium systems. With added Ca2+, tau decreases, from tau 0 at 0 added Ca2+ (mean, 29.7 +/- 3 s), by approximately 3 s at 5 mM added Ca2+. With added EDTA, tau increases sigmoidally from tau 0 at 0 EDTA to a maximum (mean tau m = 142 +/- 23 s) at approximately 200 microM EDTA. tau then decreases slightly to a minimum at approximately 1.3 mM and finally increases to infinity at approximately 10 mM EDTA. Between 0 and 1.3 mM EDTA, effects on clotting time are completely reversed by adding Ca2+ and, after equilibration at 400 microM EDTA, tau is independent of EDTA concentration. Thus, up to 400 microM EDTA, effects on clotting time are attributed to decreasing fibrinogen bound Ca2+. Between 5 mM Ca2+ and 200 microM EDTA it is assumed that an equilibrium distribution of fibrinogen species having 3, 2, 1, or 0 bound calcium ions is established and that a clotting time is determined by the sum of products of species fractional abundance and pure species clotting time. Analysis indicates that pure species clotting times increase proportionately with decreasing Ca2+ binding, binding sites are nearly independent, and the microscopic association constant for the first bound Ca2+ is approximately 4.9 X 10(6) M-1. Effects of adding Ca2+ at times t1 after thrombin addition to systems initially equilibrated at 200 microM EDTA were determined. Analysis of the relation between tau and t1 indicates that as Ca2+ binding decreases, rate constants for release of B peptides decrease less than those for release of A peptides. As EDTA concentration is increased above 1.3 mM, inhibitory effects of EDTA and CaEDTA progressively increase.

  14. Chromium-51-EDTA clearance in adults with a single-plasma sample.

    Science.gov (United States)

    Mårtensson, J; Groth, S; Rehling, M; Gref, M

    1998-12-01

    In 1996, a committee on renal clearance recommended a mean sojourn time-based methodology for single-sample determination of plasma clearance of 99mTc-diethylenetriamine pentaacetic acid (DTPA) to be used on adults if the patient's glomerular filtration rate (GFR) is suspected to be >30 ml/min. The main purpose of this study was to derive a mean sojourn time-based formula for calculation of 51Cr-ethylenediamine tetraacetic acid (EDTA) clearance in adults. Two groups of patients with 51Cr-EDTA clearance (Cl) between 16 and 172 ml/min were studied. In Group I (n = 46), reference Cl was determined as a multiplasma sample, single-injection method (ClSM). Sixteen blood samples were drawn from 0 until 5 hr after a single intravenous injection of 51Cr-EDTA. In Group II (n = 1046), reference Cl was determined by the Brøchner-Mortensen four-sample clearance method (ClBM). The plasma time-activity curves of Group I were used to derive two mean sojourn time-based formulas (Formulas 1 and 2) for calculation of a single-sample clearance. Formula 1 was derived from the entire time-activity curve, whereas the derivation of Formula 2 used only the final slope of the time-activity curve. The accuracy of the two formulas and the Christensen and Groth 99mTc-DTPA formula was tested on Group II. Chromium-51-EDTA Cl calculated by Formula 1 was almost identical to the Cl calculated by the reference Cl method (r = 0.982; SDdiff = 5.82 ml/min). Both 51Cr-EDTA Cl calculated by Formula 2 and by the 99mTc-DTPA formula showed close correlation with the reference method (r = 0.976, r = 0.985, respectively) but systematically overestimated GFR for the whole range of clearance values by 3.5 and 3.2 ml/min (ptime methodology. The determination is marginally more accurate (ptime-activity curve than from only the final slope. The single-sample formula derived for determination of 99mTc-DTPA Cl tends slightly to overestimate GFR if used to calculate 51Cr-EDTA Cl.

  15. An evaluation of EDTA additions for improving the phytoremediation efficiency of different plants under various cultivation systems.

    Science.gov (United States)

    Luo, Jie; Qi, Shihua; Gu, X W Sophie; Wang, Jinji; Xie, Xianming

    2016-05-01

    Previous studies have shown that phytoremediation usually requires soil amendments, such as chelates, to mobilize low bioavailability heavy metals for better plant absorption and, consequently, for remediation efficiency. A total dry biomass of 3.39 and 0.0138 kg per plant was produced by a phytoremediator, Eucalyptus globulus, and a nitrogen fixing crop, Cicer arietinum (chickpea), respectively. The accumulation of Pb in E. globulus and chickpea reached 1170.61 and 1.33 mg per plant (700 and 324 mg kg(-1)), respectively, under an ethylene diamine tetraacetic acid (EDTA) treatment, which was a five and sixfold increase over the value in untreated experiments, respectively. EDTA enhanced the phytoremediation efficiency and increased the heavy metal concentration in the soil solution. In pot experiments, approximately 27 % of the initial Pb leached from the spiked soil after EDTA and 25 mm artificial precipitation additions into soil without plants, which was considerably larger than the value under the same conditions without EDTA application (7 %). E. globulus planted in a mixed culture had higher water use efficiency than monocultures of either species in field experiments, and E. globulus intercepted almost all of the artificial precipitation in the pot experiments. This study demonstrates that E. globulus can maximize the potential of EDTA for improving the phytoremediation efficiency and minimizing its negative effects to the environment simultaneously by absorbing the metal-rich leachate, especially in a mixed culture of E. globulus and chickpeas.

  16. Adsorption of manganese(II) ions by EDTA-treated activated carbons

    Energy Technology Data Exchange (ETDEWEB)

    Khan, A.Y.; Mazyck, D.W. [Jones Edmunds & Associates, Gainesville, FL (United States)

    2009-07-01

    The adsorption of manganese(II) ions from aqueous solution onto three different granular activated carbons treated with ethylenediamine tetraacetic acid (EDTA) and its sodium salt was investigated. Characterization of the chelate-treated carbons showed that EDTA altered the physical and chemical properties of the sorbents relative to their untreated counterparts. Furthermore, the modified sorbents exhibited a heightened capacity towards the adsorption of Mn(II) ions from aqueous media. Manganese(II) ion removal increased from 0 to 6.5 mg/g for the lignite coal-based sorbent, from 3.5 to 14.7 mg/g for the wood-based sorbent and from 1.3 to 7.9 mg/g for the bituminous coal-based sorbent. The increased removal is attributed, in part, to the creation of Lewis base sites that participate in covalent interactions and hydrolysis reactions.

  17. The kinetics of lanthanide complexation by EDTA and DTPA in lactate media.

    Science.gov (United States)

    Nash, K L; Brigham, D; Shehee, T C; Martin, A

    2012-12-28

    The interaction of trivalent lanthanide and actinide cations with polyaminopolycarboxylic acid complexing agents in lactic acid buffer systems is an important feature of the chemistry of the TALSPEAK process for the separation of trivalent actinides from lanthanides. To improve understanding of metal ion coordination chemistry in this process, the results of an investigation of the kinetics of lanthanide complexation by ethylenediamine-N,N,N',N'-tetraacetic acid (EDTA) and diethylenetriamine-N,N,N',N'',N''-pentaacetic acid (DTPA) in 0.3 M lactic acid/0.3 M ionic strength solution are reported. Progress of the reaction was monitored using the distinctive visible spectral changes attendant to lanthanide complexation by the colorimetric indicator ligand Arsenazo III, which enables the experiment but plays no mechanistic role. Under the conditions of these experiments, the reactions occur in a time regime suitable for study by stopped-flow spectrophotometric techniques. Experiments have been conducted as a function of EDTA/DTPA ligand concentration, total lactic acid concentration, and pH. The equilibrium perturbation reaction proceeds as a first order approach to equilibrium over a wide range of conditions, allowing the simultaneous determination of complex formation and dissociation rate constants. The rate of the complexation reaction has been determined for the entire lanthanide series (except Pm(3+)). The predominant pathway for lanthanide-EDTA and lanthanide-DTPA dissociation is inversely dependent on the total lactate concentration; the complex formation reaction demonstrates a direct dependence on [H(+)]. Unexpectedly, the rate of the complex formation reaction is seen in both ligand systems to be fastest for Gd(3+). Correlation of these results indicates that in 0.3 M lactate solutions the exchange of lanthanide ions between lactate complexes and the polyaminopolycarboxylate govern the process.

  18. Silver-zinc electrodeposition from a thiourea solution with added EDTA or HEDTA

    International Nuclear Information System (INIS)

    Oliveira, G.M. de; Carlos, I.A.

    2009-01-01

    This paper shows the study of silver-zinc electrodeposition from a thiourea solution with added (ethylenedinitrilo)tetraacetic acid (EDTA), disodium salt and N-(2-hydroxyethyl)ethylenediaminetriacetic acid (HEDTA), trisodium salt. Voltammetric results indicated that silver-zinc alloy can be obtained applying overpotential higher than 0.495 V, in Tu solution containing 1.0 x 10 -1 mol L -1 Zn(NO 3 ) 2 + 2.5 x 10 -2 mol L -1 AgNO 3 . This was due to silver(I) ion complexation with thiourea, which shifted the silver deposition potential to more negative value and due to silver-zinc alloy deposition, which occurred at potentials more positive than the potential to zinc deposition alone. EDTA or HEDTA did not significantly affect the silver and zinc deposition potentials, but decreased the current density for silver-zinc deposition. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) analyses of the silver-zinc deposits showed that the morphology and composition changed as a function of the conditions of deposition, viz, deposition potential (E d ), deposition charge density (q d ) and solution composition (silver, EDTA and HEDTA concentrations). EDS analysis of the deposits showed sulphur (S) incorporated into the silver-zinc deposit, while SEM images showed that this sulphur content seemed to improve the silver-zinc morphology, as did the presence of EDTA and HEDTA in the solution, which enhanced the sulphur incorporation into the silver-zinc deposit. X-ray diffraction (XRD) analysis of the silver-zinc deposit showed that it was amorphous, irrespective of its composition and morphology

  19. The amino acid distribution in rachis xylem sap and phloem exudate of Vitis vinifera 'Cabernet Sauvignon' bunches.

    Science.gov (United States)

    Gourieroux, Aude M; Holzapfel, Bruno P; Scollary, Geoffrey R; McCully, Margaret E; Canny, Martin J; Rogiers, Suzy Y

    2016-08-01

    Amino acids are essential to grape berry and seed development and they are transferred to the reproductive structures through the phloem and xylem from various locations within the plant. The diurnal and seasonal dynamics of xylem and phloem amino acid composition in the leaf petiole and bunch rachis of field-grown Cabernet Sauvignon are described to better understand the critical periods for amino acid import into the berry. Xylem sap was extracted by the centrifugation of excised leaf petioles and rachises, while phloem exudate was collected by immersing these structures in an ethylenediaminetetraacetic acid (EDTA) buffer. Glutamine and glutamic acid were the predominant amino acids in the xylem sap of both grapevine rachises and petioles, while arginine and glycine were the principal amino acids of the phloem exudate. The amino acid concentrations within the xylem sap and phloem exudate derived from these structures were greatest during anthesis and fruit set, and a second peak occurred within the rachis phloem at the onset of ripening. The concentrations of the amino acids within the phloem and xylem sap of the rachis were highest just prior to or after midnight while the flow of sugar through the rachis phloem was greatest during the early afternoon. Sugar exudation rates from the rachis was greater than that of the petiole phloem between anthesis and berry maturity. In summary, amino acid and sugar delivery through the vasculature to grape berries fluctuates over the course of the day as well as through the season and is not necessarily related to levels near the source. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  20. Role of CA-EDTA on the Synthesizing Process of Cerate-Zirconate Ceramics Electrolyte

    OpenAIRE

    Abdullah, Nur Athirah; Hasan, Sharizal; Osman, Nafisah

    2013-01-01

    The role of a combination between citric acid (CA) and ethylenediaminetetra acetic acid (EDTA) as chelating agents in preparation of BaCe0.54Zr0.36Y0.1O2.95 powder by a modified sol-gel method is reported. The precursor solutions were prepared from metal nitrate salts (M+), chelating agents (C), and ethylene glycol (EG) at molar ratio of M+ : C : EG = 3 : 2 : 3. Chemical and phase transformation of samples during thermal decomposition were analyzed by thermogravimetric analysis (TGA) and Four...

  1. Flame-Sprayed Y2O3 Films with Metal-EDTA Complex Using Various Cooling Agents

    Science.gov (United States)

    Komatsu, Keiji; Toyama, Ayumu; Sekiya, Tetsuo; Shirai, Tomoyuki; Nakamura, Atsushi; Toda, Ikumi; Ohshio, Shigeo; Muramatsu, Hiroyuki; Saitoh, Hidetoshi

    2017-01-01

    In this study, yttrium oxide (Y2O3) films were synthesized from a metal-ethylenediaminetetraacetic (metal-EDTA) complex by employing a H2-O2 combustion flame. A rotation apparatus and various cooling agents (compressed air, liquid nitrogen, and atomized purified water) were used during the synthesis to control the thermal history during film deposition. An EDTA·Y·H complex was prepared and used as the staring material for the synthesis of Y2O3 films with a flame-spraying apparatus. Although thermally extreme environments were employed during the synthesis, all of the obtained Y2O3 films showed only a few cracks and minor peeling in their microstructures. For instance, the Y2O3 film synthesized using the rotation apparatus with water atomization units exhibited a porosity of 22.8%. The maximum film's temperature after deposition was 453 °C owing to the high heat of evaporation of water. Cooling effects of substrate by various cooling units for solidification was dominated to heat of vaporization, not to unit's temperatures.

  2. Lipid emulsion therapy: non-nutritive uses of lipid emulsions in ...

    African Journals Online (AJOL)

    However, they undergo faster ... For this reason, preservatives are commonly added to propofol. Only ethylenediaminetetraacetic acid (EDTA) and metabisulfate are currently approved by the Food and Drug administration.6 They have.

  3. Methodologic aspects of acetylcholine-evoked relaxation of rabbit aorta

    DEFF Research Database (Denmark)

    Larsen, Kirsten Vendelbo; Nedergaard, Ove A.

    1999-01-01

    The acetylcholine-evoked relaxation of rabbit isolated thoracic aorta precontracted by phenylephrine was studied. Phenylephrine caused a steady contraction that was maintained for 6 h. In the presence of calcium disodium ethylenediaminetetraacetate (EDTA) and ascorbic acid the contraction decreased...

  4. Studies on biamperometric determination of thorium using EDTA as titrant

    International Nuclear Information System (INIS)

    Jayachandran, Kavitha; Nair, P.R.; Noronha, D.M.; Xavier, Mary; Aggarwal, S.K.

    2009-01-01

    A method is described for the determination of thorium at sub milligram levels by EDTA complexometric titration using biamperometric end point detection. The methodology is based on the electroactive behaviour of EDTA observed at pH 2-3, suggesting a reversible electron transfer reaction at potential ≥ 200 mV applied between twin Pt electrodes. Accuracy and precision of better than 2% were obtained for thorium amounts in the 100-500 μg range. (author)

  5. Amperometric titration of indium with edta solution in propanol

    International Nuclear Information System (INIS)

    Gevorgyan, A.M.; Talipov, Sh.T.; Khadeev, V.A.; Kostylev, V.S.; Khadeeva, L.A.

    1980-01-01

    Optimum conditions have been chosen for titration of indium with EDTA solution in anhydrous propanol and its mixtures with some aprotic solvents using amperometric and point detection. A procedure is suggested of determining indium microcontents in the presence of large amounts of other elements. The procedure is based on its extraction preseparation followed by direct titration in the extract with a standard EDTA solution [ru

  6. Characterization and adsorption mechanism of Zn2+ removal by PVA/EDTA resin in polluted water

    International Nuclear Information System (INIS)

    Zhang Yun; Li Yanfeng; Yang Liuqing; Ma Xiaojie; Wang Liyuan; Ye Zhengfang

    2010-01-01

    Batch adsorption experiments were conducted using a PVA/EDTA resin as an adsorbent to adsorb Zn(II) ions from single component system in which experimental parameters were studied including solution pH, contact time, adsorbent dose and initial metal ions concentration. The equilibrium isotherms were determined at pH 6 under constant ionic strength and at different temperatures. The results showed that the maximum removal of Zn(II) (99.8%) with 1 g L -1 of sorbent was observed at 40 mg L -1 at an initial pH value of 6. Removals of about 60-70% occurred in 15 min, and equilibrium was attained at around 30 min. The equilibrium data for the adsorption of Zn(II) on PVA/EDTA resin was tested with various adsorption isotherm models among which three models were found to be suitable for the Zn(II) adsorption. In addition, the kinetic adsorption fitted well to the pseudo-second-order model and the corresponding rate constants were obtained. Thermodynamic aspects of the adsorption process were also investigated. Furthermore a higher desorption efficiency of Zn(II) from the PVA/EDTA resin using acid treatment was available by more than 95%.

  7. The Effect of an EDTA-based Chelation Regimen on Patients with Diabetes and Prior Myocardial Infarction in TACT

    Science.gov (United States)

    Escolar, Esteban; Lamas, Gervasio A.; Mark, Daniel B.; Boineau, Robin; Goertz, Christine; Rosenberg, Yves; Nahin, Richard L.; Ouyang, Pamela; Rozema, Theodore; Magaziner, Allan; Nahas, Richard; Lewis, Eldrin F.; Lindblad, Lauren; Lee, Kerry L.

    2014-01-01

    Background The Trial to Assess Chelation Therapy (TACT) showed clinical benefit of an ethylene diamine tetraacetic acid (EDTA-based) infusion regimen in patients 50 years or older with prior myocardial infarction (MI). Diabetes prior to enrollment was a pre-specified subgroup. Methods and Results Patients received 40 infusions of EDTA chelation or placebo. 633 (37%) had diabetes (322 EDTA, 311 placebo). EDTA reduced the primary endpoint (death, reinfarction, stroke, coronary revascularization, or hospitalization for angina) [25% vs 38%, hazard ratio (HR) 0.59, 95% confidence interval (CI) (0.44, 0.79), p<0.001] over 5 years. The result remained significant after Bonferroni adjustment for multiple subgroups (99.4% CI (0.39, 0.88), adjusted p=0.002). All-cause mortality was reduced by EDTA chelation [10% vs 16%, HR 0.57, 95% CI (0.36, 0.88) p=0.011], as was the secondary endpoint (cardiovascular death, reinfarction, or stroke) [11% vs 17% HR 0.60, 95% CI (0.39, 0.91), p=0.017]. After adjusting for multiple subgroups, however, those results were no longer significant. The number needed to treat to reduce one primary endpoint was 6.5 over 5 years (95% CI (4.4, 12.7). There was no reduction in events in non-diabetics (n=1075, p=0.877), resulting in a treatment by diabetes interaction (p=0.004). Conclusions Post-MI diabetic patients age 50 or older demonstrated a marked reduction in cardiovascular events with EDTA chelation. These findings support efforts to replicate these findings and define the mechanisms of benefit. They do not, however, constitute sufficient evidence to indicate the routine use of chelation therapy for all post-MI diabetic patients. PMID:24254885

  8. Treatment of Ni-EDTA containing wastewater by electrocoagulation using iron scraps packed-bed anode.

    Science.gov (United States)

    Ye, Xiaokun; Zhang, Junya; Zhang, Yan; Lv, Yuancai; Dou, Rongni; Wen, Shulong; Li, Lianghao; Chen, Yuancai; Hu, YongYou

    2016-12-01

    The unique electrocoagulator proposed in this study is highly efficient at removing Ni-EDTA, providing a potential remediation option for wastewater containing lower concentrations of Ni-EDTA (Ni ≤ 10 mg L -1 ). In the electrocoagulation (EC) system, cylindrical graphite was used as a cathode, and a packed-bed formed from iron scraps was used as an anode. The results showed that the removal of Ni-EDTA increased with the application of current and favoured acidic conditions. We also found that the iron scrap packed-bed anode was superior in its treatment ability and specific energy consumption (SECS) compared with the iron rod anode. In addition, the packed density and temperature had a large influence on the energy consumption (ECS). Over 94.3% of Ni and 95.8% of TOC were removed when conducting the EC treatment at an applied current of 0.5 A, initial pH of 3, air-purged rate 0.2 L min -1 , anode packed density of 400 kg m -3 temperature of 313 K and time of 30 min. SEM analysis of the iron scraps indicated that the specific area of the anode increased after the EC. The XRD analysis of flocs produced during EC revealed that hematite (α-Fe 2 O 3 ) and magnetite (Fe 3 O 4 ) were the main by-products under aerobic and anoxic conditions, respectively. A kinetic study demonstrated that the removal of Ni-EDTA followed a first-order model with the current parameters. Moreover, the removal efficiency of real wastewater was essentially consistent with that of synthetic wastewater. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Role of organic acids in enhancing the desorption and uptake of weathered p,p'-DDE by Cucurbita pepo

    International Nuclear Information System (INIS)

    White, Jason C.; Mattina, MaryJane Incorvia; Lee, W.-Y.; Eitzer, Brian D.; Iannucci-Berger, William

    2003-01-01

    The addition of low molecular weight organic acids to soil may enhance phytoremediation of persistent organic pollutants. - Experiments were conducted to assess the effect of seven organic acids [succinic, tartaric, malic, malonic, oxalic, citric, ethylenediaminetetraacetic (EDTA)] over a concentration range of two orders of magnitude (0.001-0.10 M) on the abiotic desorption of weathered p,p'-DDE and the extraction of polyvalent inorganic ions from soil. At 0.05 M all organic acids significantly increased contaminant desorption by 19-80%. Organic acids also increased the aqueous concentration of eight inorganic constituents extracted from soil, with at least a six-fold increase in the release of Al, Fe, Mn, and P at 0.001 M. Zucchini seedlings grown for 28 d in soil containing weathered p,p'-DDE (300 ng/g, dry weight) were periodically amended with distilled water, citric or oxalic acids (0.01 M). Plants receiving water removed 1.7% of the p,p'-DDE from the soil. Seedlings amended with citric or oxalic acids removed 2.1 and 1.9% of the contaminant, respectively, and contained up to 66% more contaminant in the shoot system than unamended vegetation. A second crop of untreated (distilled water) zucchini in the same soil removed more contaminant than the first crop (2.5%), although the addition of organic acids did not further enhance contaminant uptake. The data indicate that the addition of low molecular weight organic acids causes the partial dissolution of the soil structure through the chelation of inorganic structural ions, potentially enhancing bioavailability and having implications for the phytoremediation of persistent organic pollutants in soil

  10. CaEDTA vs CaEDTA plus BAL to treat children with elevated blood lead levels.

    Science.gov (United States)

    O'Connor, M E

    1992-07-01

    The effectiveness of CaEDTA alone vs CaEDTA plus BAL was compared retrospectively in a group of 72 children with lead levels between 2.41 mumol/L (50 micrograms/dL) and 2.90 mumol/L (60 micrograms/dL). The children who received both drugs had higher median zinc protoporphyrin (ZnP) concentrations at the initiation of therapy than children who received CaEDTA alone (160 micrograms/dL vs 96 micrograms/dL, p less than .01). There was a significantly increased incidence of vomiting and abnormal liver-function test results in the children who received both drugs. The children who received CaEDTA alone had a greater percent mean fall in lead level at one to three weeks postchelation (30.5% vs 18.1%, p less than .05). Children who received both CaEDTA and BAL had a greater percent decrease in ZnP at four to eight months postchelation, but there was no difference in percent decrease in lead levels. Children who received both drugs also had a greater number of repeat courses of chelation by six months. The addition of BAL to CaEDTA for treatment of children with lead levels of 2.41 mumol/L (50 micrograms/dL) to 2.90 mumol/L (60 micrograms/dL) produced greater toxicity and does not seem to prevent repeat chelations within six months.

  11. Comparative in vitro studies on disodium EDTA effect with and without Proteus mirabilis on the crystallization of carbonate apatite and struvite

    Science.gov (United States)

    Prywer, Jolanta; Olszynski, Marcin; Torzewska, Agnieszka; Mielniczek-Brzóska, Ewa

    2014-06-01

    Effect of disodium EDTA (salt of ethylenediamine tetraacetic acid) on the crystallization of struvite and carbonate apatite was studied. To evaluate such an effect we performed an experiment of struvite and carbonate apatite growth from artificial urine. The crystallization process was induced by Proteus mirabilis to mimic the real urinary tract infection, which usually leads to urinary stone formation. The results demonstrate that disodium EDTA exhibits the effect against P. mirabilis retarding the activity of urease - an enzyme produced by these microorganisms. The spectrophotometric results demonstrate that, with and without P. mirabilis, the addition of disodium EDTA increases the induction time and decreases the growth efficiency compared to the baseline (without disodium EDTA). These results are discussed from the standpoint of speciation of complexes formed in the solution of artificial urine in the presence of disodium EDTA. The size of struvite crystals was found to decrease in the presence of disodium EDTA. However, struvite crystals are larger in the presence of bacteria while the crystal morphology and habit remain unchanged.

  12. Kinetic study of the substitution of [Tc(tu)6]3+ by polyaminocarboxylic acids

    International Nuclear Information System (INIS)

    Torres, J.; Gonalez, R.; Kremer, C.; Kremer, E.; Leon, A.

    1997-01-01

    Substitution route has been traditionally proposed for the synthesis of new Tc complexes. In particular, Tc(III) coordination compounds can be successfully prepared by substitution on [Tc(tu) 6 ] 3+ (tu = thiourea). In this substitution reaction, TcO 2 is a side product that should be minimized. The success of these synthetic procedures is based on controlling the reaction conditions in order that pure substitution could be faster enough compared to decomposition. In this work, the substitution of [Tc(tu) 6 ] 3+ by polycarboxylic acids (ethylenediaminetetraacetate (edta), diethylenetriaminepentaacetate (dtpa), N-tris(2-amino-ethyl)amin N', N', N '' , N '' , N ''' , N ''' -hexaacetate (ttaha) and 3-bromo-2,4,6-trimethylacetanilideiminodiacetate (mebrofenin)) is kinetically studied in order to obtain the best conditions for preparing Tc(III) complexes. As a general conclusion, substitution constants are strongly dependent on the pH. k obs values fall in the range 0.1-13 x 10 -2 M -1 s -1 , depending on the pH value and the temperature. No strong differences are found with different incoming ligands. For the four ligands (L), a straight line is obtained when plotting log k obs /([L m- ]) vs. pH. This is consistent with a rate constant k obs = {k 0 + Σk n [H + ] n /K n }[L m- ], where n represents the charge of the incoming ligand and m is the maximum value of n. (orig.)

  13. Behavior of lead and zinc in plasma, erythrocytes, and urine and ALAD in erythrocytes following intravenous infusion of CaEDTA in lead workers.

    Science.gov (United States)

    Araki, S; Aono, H; Fukahori, M; Tabuki, K

    1984-01-01

    To evaluate the effect of calcium disodium ethylenediamine tetraacetate (CaEDTA) on concentrations of lead and zinc in plasma, erythrocytes, whole blood, and urine, we administered CaEDTA by intravenous infusion for 1 hr to seven lead workers with blood lead concentrations of 46-67 micrograms/100 g (mean 54 micrograms/100 g). The plasma lead concentration (PPb) and the mobilization yield of lead in urine by CaEDTA were highest during the period between 1 and 2 hr after the infusion was started. In contrast, the lead concentration in erythrocytes (EPb) and in whole blood (BPb) remained unchanged during the 24 hr following infusion. Plasma zinc concentration (PZn) also fell rapidly following CaEDTA infusion; the decline was followed by a gradual rise in the zinc concentration in erythrocytes (EZn) without alteration in the zinc in whole blood. The mobilization yield of zinc in urine by CaEDTA (MZn) reached its highest level within 1 hr after the start of the infusion. Delta-aminolevulinic acid dehydratase (ALAD) activity in erythrocytes gradually increased for 5 hr following CaEDTA infusion. These observations suggest that (1) PPb concentration is a more sensitive indicator of the body burden of chelatable lead than is either BPb or EPb; (2) MZn is mobilized mostly from plasma during the first several hours following the start of CaEDTA infusion, and the fall in PZn concentration following infusion is compensated first by a rise in EZn concentration and then by an immediate redistribution of zinc in other organs to the blood; and (3) Pb-inhibited ALAD activity is reactivated by the increased EZn during and shortly after CaEDTA infusion.

  14. EDTA chelation therapy for cardiovascular disease: a systematic review

    Directory of Open Access Journals (Sweden)

    Wu Ping

    2005-11-01

    Full Text Available Abstract Background Numerous practitioners of both conventional and complementary and alternative medicine throughout North America and Europe claim that chelation therapy with EDTA is an effective means to both control and treat cardiovascular disease. These claims are controversial, and several randomized controlled trials have been completed dealing with this topic. To address this issue we conducted a systematic review to evaluate the best available evidence for the use of EDTA chelation therapy in the treatment of cardiovascular disease. Methods We conducted a systematic review of 7 databases from inception to May 2005. Hand searches were conducted in review articles and in any of the trials found. Experts in the field were contacted and registries of clinical trials were searched for unpublished data. To be included in the final systematic review, the studies had to be randomized controlled clinical trials. Results A total of seven articles were found assessing EDTA chelation for the treatment of cardiovascular disease. Two of these articles were subgroup analyses of one RCT that looked at different clinical outcomes. Of the remaining five studies, two smaller studies found a beneficial effect whereas the other three exhibited no benefit for cardiovascular disease from the use of EDTA chelation therapy. Adverse effects were rare but those of note included a few cases of hypocalcemia and a single case of increased creatinine in a patient on the EDTA intervention. Conclusion The best available evidence does not support the therapeutic use of EDTA chelation therapy in the treatment of cardiovascular disease. Although not considered to be a highly invasive or harmful therapy, it is possible that the use of EDTA chelation therapy in lieu of proven therapy may result in causing indirect harm to the patient.

  15. Role of reducing agent in extraction of arsenic and heavy metals from soils by use of EDTA.

    Science.gov (United States)

    Kim, Eun Jung; Jeon, Eun-Ki; Baek, Kitae

    2016-06-01

    Although many metal-contaminated sites contain both anionic arsenic and cationic heavy metals, the current remediation technologies are not effective for the simultaneous removal of both anionic and cationic elements from the contaminated sites due to their different characteristics. In this study, the role of reducing agent in simultaneous extraction of As, Cu, Pb, and Zn from contaminated soils was investigated using EDTA. The addition of reducing agents, which includes sodium oxalate (Na2C2O4), ascorbic acid (C6H8O6) and sodium dithionite (Na2S2O4), greatly enhanced the EDTA extraction of both As and heavy metals from the contaminated soils due to the increased mobility of the metals under the reduced conditions. The extent of the enhancement of the EDTA extraction was greatly affected by the reducing conditions. Strong reducing conditions (0.1 M of dithionite) were required for the extraction of metals strongly bound to the soil, while weak reducing conditions (0.01 M of dithionite or 0.1 M of oxalate/ascorbic acid) were sufficient for extraction of metals that were relatively weakly bound to the soil. An almost 90% extraction efficiency of total metals (As, Cu, Zn, and Pb) was obtained from the contaminated soils using the combination of dithionite and EDTA. Our results clearly showed that the combination of dithionite and EDTA can effectively extract As and heavy metals simultaneously from soils under a wide range of pH conditions. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Antibacterial activity of nisin, oregano essential oil, EDTA, and their combination against Salmonella Enteritidis for application in mayonnaise | Avaliação de atividade antibacteriana de nisina, óleo essencial de orégano, EDTA e sua combinação contra Salmonella Enteritidis para aplicação na maionese

    Directory of Open Access Journals (Sweden)

    Janine Passos Lima da Silva

    2016-04-01

    Full Text Available Salmonella Enteritidis (SE is one of the most important serovars associated with Salmonella gastroenteritis outbreaks in Brazil. The use of natural antimicrobials can be an alternative method of SE control. The antimicrobial effect of two oregano essential oils (OEO1 and OEO2 at 0.1%, 0.2%, 0.5%, 1.0%, or 2.0%; nisin (Nisaplin® at 0, 6.25, 12.5, or 25 ppm; ethylenediaminetetraacetic acid (EDTA at 0.0037%, 0.0056%, 0.0075%, 0.0110%, or 0.0150%; and their combination against SE in vitro was studied to be applied in mayonnaise and Russian salad made with the same mayonnaise during storage at 8º or 30ºC for 24 hours. OEO was very efficient against SE at all tested concentrations, while nisin and EDTA showed no effect against SE. Ten volatile components were identified in the two OEOs using gas chromatography coupled to mass spectrometry with electron impact ionization, with carvacrol being the major component of both samples. OEO2, containing p-cymene (15.95% and y-terpinene (6.90%, besides carvacrol (61.66%, resulted in larger inhibition zone than the other OEO (OEO1 don’t contains p-cymene or y-terpinene. Nisin in combination with OEO1 or OEO2 had an antagonistic effect at all concentrations. The presence of nisin caused a reduction in essential oil antimicrobial activity (p≤0.05. Sensory evaluation showed that consumers prefer 0.2% OEO in mayonnaise instead of 0.5% and 1.0% concentrations. Thus, OEO only, at a concentration of 0.2%, was applied in mayonnaise against SE. The Russian salad prepared with mayonnaise plus OEO at 0.2% (wt/wt caused a reduction of SE when compared with the salad prepared with mayonnaise without OEO. These results indicate that the use of OEO as a biopreservative (natural antimicrobial can enhance food safety, serving as an additional barrier in helping the Good Manufacturing Practices and the Hazard Analysis Critical Control Point program, fundamental to food safety

  17. Preparation and chemical studies on Tc(III) complexes containing polyaminocarboxylic acids

    International Nuclear Information System (INIS)

    Gonzalez, R.; Kremer, C.; Chiozzone, R.; Torres, J.; Rivero, M.; Kremer, E.; Leon, A.

    1998-01-01

    Tc(III)-edta (edta = ethylenediaminetetraacetate), Tc(III)-dtpa (dtpa = diethylenetriaminepentaacetate) and Tc(III)-mebrofenin (mebrofenin = 3-bromo-2,4,6-trimethylacetanilideiminodiacetate) complexes were prepared by ligand substitution reactions on hexakis(thiourea)technetium(III) cation with the polycarboxylates. By a combination of different techniques (UV-Vis, IR and 1 H-NMR spectroscopy, Tc elemental analyses and cerimetric titrations) it was concluded that 1:1 complexes are formed with edta and dtpa while mebrofening adopts a 2:1 (ligand to metal) stoichiometry. Complementary, molecular mechanics calculations were performed to analyze the spatial arrangement of the molecules. The stability of the Tc(III) complexes was studied in aqueous solution by paper chromatography, paper electrophoresis and cyclic voltammetry. The chelates are rather stable, in particular when compared with the precursor. (orig.)

  18. Separation and preconcentration of lead(II), cobalt(II), and nickel(II) on EDTA immobilized activated carbon cloth prior to flame atomic absorption spectrometric determination in environmental samples

    OpenAIRE

    ALOTHMAN, ZEID ABDULLAH; YILMAZ, ERKAN; HABİLA, MOHAMED; SOYLAK, MUSTAFA

    2015-01-01

    The synthesis and characterization of ethylenediaminetetraacetic acid immobilized activated carbon cloth was performed in the present work. It was used for preconcentration-separation of lead(II), cobalt(II), and nickel(II) at trace levels as an adsorbent. Factors including pH, concentration and volume of eluent, sample and eluent flow rates, sample volume, and effect of coexisting ions on the solid phase extraction of analytes were examined. The preconcentration factor was 50. The detection ...

  19. Simultaneous absorption of NO and SO{sub 2} into Fe-II-EDTA solution coupled with the Fe-II-EDTA regeneration catalyzed by activated carbon

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, H.S.; Mao, Y.P.; Yang, X.J.; Chen, Y.; Long, X.L.; Yuan, W.K. [East China University of Science & Technology, Shanghai (China)

    2010-07-30

    The simultaneous removal of NO and SO{sub 2} from flue gases can be realized with Fe(II)-ethylenediamineteraacetate (EDTA) solution. Activated carbon is used to catalyze the reduction of Fe-III-EDTA to Fe-II-EDTA to maintain the capability of removing NO of the Fe-EDTA solution. The reductant is the sulfite/bisulfite ions produced by SO{sub 2} dissolving into the aqueous solution. Experiments have been performed to determine the effects of activated carbon of coconut shell, Fe-II-EDTA concentration, Fe/EDTA molar ratio, SO{sub 2} partial pressure, NO partial pressure and SO{sub 4}{sup 2-} concentration on the combined elimination of NO and SO{sub 2} with Fe-II-EDTA solution coupled with the Fe-II-EDTA regeneration catalyzed by activated carbon. According to the experimental results, activated carbon not only catalyzes the reduction of Fe-III-EDTA by sulfite/bisulfite greatly but also avoids the release of N{sub 2}O. The NO removal efficiency increases with the initial Fe-II-EDTA concentration and SO{sub 2} partial pressure. The ratio of Fe/EDTA and the SO{sub 4}{sup 2-} concentration has little effect on the catalytic reduction of Fe-III-EDTA. The optimal initial NO concentration range is from 600 ppm to 900 ppm. The experimental results manifest that the Fe-II-EDTA solution coupled with catalytic regeneration of Fe-II-EDTA can maintain high nitric oxide removal efficiency for a long period of time.

  20. Phytoextraction of Pb and Cu contaminated soil with maize and microencapsulated EDTA.

    Science.gov (United States)

    Xie, Zhiyi; Wu, Longhua; Chen, Nengchang; Liu, Chengshuai; Zheng, Yuji; Xu, Shengguang; Li, Fangbai; Xu, Yanling

    2012-09-01

    Chelate-assisted phytoextraction using agricultural crops has been widely investigated as a remediation technique for soils contaminated with low mobility potentially toxic elements. Here, we report the use of a controlled-release microencapsulated EDTA (Cap-EDTA) by emulsion solvent evaporation to phytoremediate soil contaminated with Pb and Cu. Incubation experiments were carried out to assess the effect of Cap- and non-microencapsulated EDTA (Ncap-EDTA) on the mobility of soil metals. Results showed EDTA effectively increased the mobility of Pb and Cu in the soil solution and Cap-EDTA application provided lower and more constant water-soluble concentrations of Pb and Cu in comparison with. Phytotoxicity may be alleviated and plant uptake of Pb and Cu may be increased after the incorporation of Cap-EDTA. In addition phytoextraction efficiencies of maize after Cap- and Ncap-EDTA application were tested in a pot experiment. Maize shoot concentrations of Pb and Cu were lower with Cap-EDTA application than with Ncap-EDTA. However, shoot dry weight was significantly higher with Cap-EDTA application. Consequently, the Pb and Cu phytoextraction potential of maize significantly increased with Cap-EDTA application compared with the control and Ncap-EDTA application.

  1. Dissolution of Fe(III) (hydr) oxides by metal-EDTA complexes

    Science.gov (United States)

    Ngwack, Bernd; Sigg, Laura

    1997-03-01

    The dissolution of Fe(III)(hydr)oxides (goethite and hydrous ferric oxide) by metal-EDTA complexes occurs by ligand-promoted dissolution. The process is initiated by the adsorption of metal-EDTA complexes to the surface and is followed by the dissociation of the complex at the surface and the release of Fe(III)EDTA into solution. The dissolution rate is decreased to a great extent if EDTA is complexed by metals in comparison to the uncomplexed EDTA. The rate decreases in the order EDTA CaEDTA ≫ PbEDTA > ZnEDTA > CuEDTA > Co(II)EDTA > NiEDTA. Two different rate-limiting steps determine the dissolution process: (1) detachment of Fe(III) from the oxide-structure and (2) dissociation of the metal-EDTA complexes. In the case of goethite, step 1 is slower than step 2 and the dissolution rates by various metals are similar. In the case of hydrous ferric oxide, step 2 is rate-limiting and the effect of the complexed metal is very pronounced.

  2. Separation of digesta fractions complicates estimation of ileal digestibility using marker methods with Cr2O3 and Co-EDTA in broiler chickens

    NARCIS (Netherlands)

    Vries, de S.; Kwakkel, R.P.; Pustjens, A.M.; Kabel, M.A.; Hendriks, W.H.; Gerrits, W.J.J.

    2014-01-01

    Marker methodologies to measure ileal and total tract digestibilities of diets varying in content and degradability of dietary fiber in broiler chickens were evaluated. Chromium sesquioxide (Cr2O3) and cobalt-ethylenediamine tetraacetic acid (Co-EDTA) were used as markers of solid and soluble

  3. EFFECTS OF EDTA ON LEA indica) SEEDLINGS REPLANTE ECTS ...

    African Journals Online (AJOL)

    userpc

    Lead (Pb) is one of the widely distributed an most abundant toxic elements in the soil. exerts adverse effects on ... on morphology, growth esses of plants. 1975 ... l plants. The harvesting or EDTA. The pH ntration of Pb2+ before er harvest. The weights .... reported that toxicity symptoms in Indian mustard exposed to Pb and.

  4. Measurement of intestinal permeability using 51Cr-EDTA

    International Nuclear Information System (INIS)

    Peled, Y.; Watz, C.; Gilat, T.

    1985-01-01

    Intestinal permeability tests are a new class of tests of which the 51 Cr-EDTA seemed the easiest to perform. The authors have evaluated the 8- and 24-h urinary excretion of ingested 51 Cr-EDTA in 38 subjects. Twenty-seven patients with diseases not affecting the integrity of the small bowel served as controls. The test group consisted of two patients with celiac, six with Crohn's disease, and three with ulcerative colitis. Nineteen (70.37%) of the control patients had an abnormal test (more than 2.6% in 24 h). The patients with ulcerative colitis had normal excretion (mean 1.95% in 24 h). The patients with celiac disease had an elevated excretion (mean 3.62% in 24 h) and five out of six patients with Crohn's disease also had an increased excretion (mean 6.2% in 24 h). The elevated 51 Cr-EDTA excretion in the control patients casts serious doubts on the validity of the test. Possible causes for abnormal excretion in the controls include various medications used by the patients as well as changes in the chromatographic mobility of 51 Cr-EDTA, demonstrated after incubation with gastric juice

  5. Effect of Different Protocols in Preconditioning With EDTA in Sclerotic Dentin and Enamel Before Universal Adhesives Applied in Self-etch Mode.

    Science.gov (United States)

    Martini, E C; Parreiras, S O; Gutierrez, M F; Loguercio, A D; Reis, A

    The aim of this study was to investigate the effect of different protocols of 17% ethylene diamine tetra-acetic acid (EDTA) conditioning on the etching pattern and immediate bond strength of universal adhesives to enamel and sclerotic dentin. Forty bovine teeth with sclerotic dentin and 20 human third molars were randomly divided into eight groups resulting from the combination of the main factors surface treatment (none, two-minute EDTA conditioning manual application, 30-second EDTA manual application, 30-second EDTA sonic application) and adhesives systems (Scotchbond Universal Adhesive [SBU] and Prime & Bond Elect [PBE]). Resin-dentin and enamel-dentin bond specimens were prepared and tested under the microtensile bond strength (μTBS) and microshear bond strength (μSBS) tests, respectively. The etching pattern produced on the unground enamel and the sclerotic dentin surfaces under the different protocols and adhesive systems was evaluated under scanning electron microscopy. For enamel, only the main factor adhesive was significant (padhesives in the self-etch mode on sclerotic dentin, mainly when applied for 30 seconds with the aid of a sonic device. EDTA pretreatment also improves the retentive etching pattern of enamel, but it does not result in higher enamel bond strength.

  6. Kinetics of electrophilic substitution of erbium (3) for ytterbium (3) in aqueous solution of ethylenediaminetetraacetate

    Energy Technology Data Exchange (ETDEWEB)

    Nikitenko, S.I.; Martynenko, L.I.; Pechurova, N.I.; Spitsyn, V.I. (Moskovskij Gosudarstvennyj Univ. (USSR))

    1983-08-01

    The results obtained while studing the exchange kinetics in the ErA/sup -/ - Yb/sup 3 +/ system are compared with the kinetic regularities found for the NdA/sup -/ - Yb/sup 3 +/ system. Electrophilic substitution in ethylenediamine tetraacetates of rare earths (3) independently of the nature of a departing cation is realized through two competitive mechanisms: acid dissociation and associative mechanisms. Exchange through the acid dissociation mechanisms is catalized by protons while, depending on the medium acidity, mono- and biprotonated intermediate complexes are formed. The associative mechanism predominates in less acid media and is realized through the formation of intermediate binuclear complexes. The limiting exchange stage is a break in the metal-nitrogen bond formed by the departing cation and ligand in the intermediate binuclear complex.

  7. Kinetics of electrophilic substitution of erbium (3) for ytterbium (3) in aqueous solution of ethylenediaminetetraacetate

    International Nuclear Information System (INIS)

    Nikitenko, S.I.; Martynenko, L.I.; Pechurova, N.I.; Spitsyn, V.I.

    1983-01-01

    The results obtained while stUdying the exchange kinetics in the ErA - - Yb 3+ system are compared with the kinetic regularities found for the NdA - - Yb 3+ system. Electrophilic substitution in ethylenediamine tetraacetates of rare earths (3) independently of the nature of a departing cation is realized through two competitive mechanisms: acid dissociation and associatiVe mechanisms. Exchange through the acid dissociation mechanisms is catalized by protons while, depending on the medium acidity, mono- and biprotonated intermediate complexes are formed. The associative mechanism predominates in less acid media and is realized through the formation of intermediate binOclear compleXes. The limiting exchange stage is a break in the metal-nitrogen bond formed by the departing cation and ligand in the intermediate binUclear complex

  8. Thermometric titration of thorium with EDTA in the presence of large excess of neutral sodium salts

    International Nuclear Information System (INIS)

    Doi, K.

    1980-01-01

    The thermometric titration of Th(IV) in the presence of neutral sodium salts, sulphuric acid or acetic acid with EDT has been studied. The effect of each on the observed heat values for the titration is discussed. For sodium perchlorate media, ΔH values of -9 and -21 kJ/mole have been estimated for the formation of the Th(IV)-EDTA chelate at μ → 0 and μ = 0.5 (NaClO 4 ), respectively. The -ΔH values increase steadily with increase in concentration of sodium perchlorate up to at least 3M. For the titration of Th(IV) in the presence of a large excess of sodium nitrate the use of sodium iodide as a masking reagent has been examined: large amounts of Bi and Cu(II) are masked and a masking effect is observed for small amounts of Ni. (author)

  9. Plasma electrolytic oxidation of A1050 aluminium alloy in homogeneous silicate-alkaline electrolytes with edta{sup 4−} complexes of Fe, Co, Ni, Cu, La and Ba under alternating polarization conditions

    Energy Technology Data Exchange (ETDEWEB)

    Rogov, A.B., E-mail: alex-lab@bk.ru [Nikolaev Institute of Inorganic Chemistry, 3, Acad. Lavrentiev Ave., Novosibirsk, 630090 (Russian Federation); Scientific and Technical Centre “Pokrytie-A” (OOO), 15, Dzerzhinskogo Ave., Novosibirsk, 630015 (Russian Federation)

    2015-11-01

    This work is devoted to the synthesis of coatings containing a number of transition elements by plasma electrolytic oxidation (PEO) on aluminium A1050 alloy. The paper discusses PEO coatings obtained in silicate-alkaline electrolytes containing complexes of Fe, Co, Ni, Cu, La and Ba with ethylenediaminetetraacetic anion edta{sup 4−}. It is also focused on the chemical basis of the electrolyte components choice and their role in the process of PEO. Possible mechanism of coating formation process is also discussed. Alternating current mode (symmetrical sinusoidal current pulses, initial average current density - 100 mA cm{sup −2}) was used to produce the coatings. The PEO process was characterized by behaviours of the anodic and cathodic peak voltage curves. Coating surfaces and cross sections are studied by optical dark field microscopy and scanning electron microscopy, X-ray and energy dispersive analysis. - Highlights: • Alkaline homogeneous electrolyte with transition metal-edta{sup 4-} complexes. • Coatings contain Fe, Co, Ni, Cu, La, Ba elements in alumina-silica matrix. • Alternating symmetric sinusoidal current of 100 mA cm{sup −2} was applied. • Borax buffer solution and silicate passivating agent were used.

  10. Inhibition and Kinetic Studies of Tortoise (Kinixys erosa) Liver arginase

    African Journals Online (AJOL)

    The effect of amino acid on tortoise liver arginase showed that L-lysine, L-valine, L-serine, L-aspartic acid and L aspartic acid had significant inhibitory effect on the enzyme but proline and glutamic acid showed slight inhibition. Ethylenediaminetetraacetic acid (EDTA), citrate, ascorbic acid, boric acid and sodium borate ...

  11. In-EDTA as activable tracer in hydrogeological investigations

    International Nuclear Information System (INIS)

    Stanescu, S.P.; Gaspar, E.; Spiridon, S.; Farcasiu, O.M.; Catilina, R.

    1982-12-01

    Two experiments are presented, on the possibilities of the use of indium in the form of the In-EDTA complex, as an activable tracer for hydrogeological studies. The determination of indium concentrations in the sampled water has been carried out by using the coprecipitation of indium with bismuth hydroxide, the neutron activation at the VVR-S reactor of the Institute for Nuclear Physics and Engineering - Bucharest and the measurement on the 417.0 keV line of sup(116m)In with the Ge(Li) spectrometric device. The advantages of the utilization of In-EDTA as a tracer for marking large volumes of water and of some long transit waters (of the order of months) have resulted. (authors)

  12. Thermometric studies on the Fe(III)-EDTA chelate.

    Science.gov (United States)

    Dot, K

    1978-02-01

    A DeltaH of -11.5 +/- 0.5 kJ/mole has been determined for the formation of the Fe(III)-EDTA chelate at 25.0 degrees and mu = 0.1(= [HClO(4)] + [NaClO(4)]) by a direct thermometric titration procedure. The entropy change, DeltaS, has been calculated to be 440 J.mole(-1) .deg(-1) by combining the result of the heat measurements with the free energy change obtained from the stability constant previously determined. A relationship between the DeltaS values and the standard partial molal entropies of the tervalent metal ions is discussed. In addition, conditions for the thermometric titration of Fe(III) with NA(4)EDTA at room temperature have been investigated. Iron(III) can be determined in the presence of fairly large amounts of phosphate, Cr(III), Mn(II) and Al(III).

  13. Application of cerium(IV)/EDTA complex for future biotechnology

    International Nuclear Information System (INIS)

    Sumaoka, Jun; Chen Wen; Kitamura, Yoshihito; Tomita, Takafumi; Yoshida, Junya; Komiyama, Makoto

    2006-01-01

    A new artificial system for site-selective hydrolysis of single-stranded DNAs was prepared. By using two oligonucleotide additives that bear a monophosphate group at the termini, gap structures were formed at predetermined positions in substrate DNA. The phosphodiester linkages in the gap were efficiently and selectively hydrolyzed by Ce(IV)/EDTA complex (EDTA, ethylenediamine-N,N,N',N'-tetraacetate) at pH 7.0 and 37 deg. C. Furthermore, the fragments formed by the site-selective scission were connected with various oligonucleotides by using T4 DNA ligase, producing desired recombinant DNAs. A new tool for manipulation of single-stranded DNA in biotechnology has been successfully obtained

  14. EDTA fouling in dead-end ultrafiltration of low level radioactive wastewater

    Energy Technology Data Exchange (ETDEWEB)

    Niu, Lixia [Collaborative Innovation Center of Advanced Nuclear Energy Technology, Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084 (China); State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (MARC), Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055 (China); Zhang, Xue [Collaborative Innovation Center of Advanced Nuclear Energy Technology, Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084 (China); Zhao, Xuan, E-mail: zhxinet@mail.tsinghua.edu.cn [Collaborative Innovation Center of Advanced Nuclear Energy Technology, Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084 (China); Hu, Hongying [Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084 (China); State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (MARC), Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055 (China)

    2015-12-15

    Highlights: • EDTA in LLRW caused unrecoverable UF membrane fouling. • The rejection of nuclides by UF was significantly enhanced with EDTA addition. • The nuclide (except Ag) deposition on membrane increased with EDTA addition. • Reducing EDTA in the feed water or alkaline/ultrasonic washing were suggested. - Abstract: EDTA is widely used as a detergent, and finally enters into wastewater. The influence of EDTA on ultrafiltration of low level radioactive wastewater (LLRW) was investigated under different operation conditions. As the main organic pollutant, EDTA led to unrecoverable membrane fouling and the normalized flux decreased from 100% to 85% depending on its concentration. The clogging caused by EDTA increased the surface roughness of the membrane, leading to the flux reduction. Both nuclide rejections and depositions on the membrane surfaces were enhanced with EDTA addition, due to the strong complexation of the nuclides with EDTA. However, Ag deposition on the membrane decreased slightly in the presence of EDTA, which may be caused by the stronger attraction of Ag to the unmodified membrane than that to the EDTA-modified one. Transmembrane pressure (TMP) and molecular weight cut off (MWCO) of membranes had negligible effects on membrane fouling, while the nuclide rejections by membrane and the depositions of nuclides on membrane both decreased significantly when the TMP increased to 0.2 MPa and MWCO increased from 5 kDa to 30 kDa. Based on these results, it clearly showed that EDTA even at a low concentration had strong effects on the performance of UF treating LLRW. Therefore, it is suggested for industrial application that pretreatments to reduce EDTA or alkaline/ultrasonic washing involved in UF process were necessary to reduce the nuclide depositions on the membrane surfaces and irradiation dose of membrane surface.

  15. Phytoremediation of lead by jack beans on a Rhodic Hapludox amended with EDTA Fitorremediação de chumbo por feijão-de-porco em um Latossolo Vermelho Distrófico tratado com EDTA

    Directory of Open Access Journals (Sweden)

    Bruno Fernando Faria Pereira

    2010-01-01

    Full Text Available A greenhouse study was conducted to evaluate the effects of ethylenediamine tetraacetic acid (EDTA addition to soils on the lead (Pb phytoextraction potential of jack beans (Canavalia ensiformis L.. In a pot experimentSoil samples (dystrophic Rhodic Hapludox were treated with six Pb rates (0, 100, 200, 350, 1,200, and 2,400 mg kg-1 soil applied as Pb(NO32 without and with EDTA application (0 and 0.5 g kg-1, respectively. Lead, Cl-, NO3-, NH4+, SO4(2-, H2PO4-, Zn2+, Cu2+, Fe3+, Al3+, Ca2+, Mg2+, K+ and DOC (dissolved organic carbon concentrations obtained in a saturation soil extract (soil:water ratio of 1:0.3 were used for Pb speciation by means of the software Visual-Minteq 2.30. Soil Pb-availability was assessed with Diethylene triamine pentaacetic acid (DTPA extraction. EDTA treated soils showed higher Pb (as PbEDTA2-, and Fe (as FeEDTA- concentrations in soil solution leading to higher uptake of these elements by the jack bean. On the other hand, it decreased the concentration of stable complexes as Pb-DOC and Fe-DOC. EDTA also induced better nutrition to plants building up the concentration of non target metals (Ca, K, Mg, Zn, Cu, Fe and Mn in shoots. Shoot dry matter yield remained constant even at the highest Pb rates after EDTA treatment. Jack bean can be considered as a potential Pb-phytoextractor. In addition, the DTPA solution was effective to assess Pb availability to the plants at all applied Pb rates.Este estudo teve como objetivo avaliar, em condições de casa de vegetação, os efeitos do ácido etilenodiamino tetraacético (EDTA no potencial fitoextrator do feijão-de-porco (Canavalia ensiformis L.. Amostras de um Latossolo Vermelho distrófico foram tratadas com seis doses de Pb (0, 100, 200, 350, 1.200 e 2.400 mg kg-1 de solo aplicadas como Pb(NO32 com e sem a aplicação de EDTA (0 e 0,5 g kg-1, respectivamente e colocadas em vasos. A concentração de Pb2+, Cl-, NO3-, NH4+, SO4(2-, H2PO4-, Zn2+, Cu2+, Fe3+, Al3+, Ca2+, Mg2

  16. Radiopharmaceuticals of DTPA, DMSA and EDTA labeled with holmium-166

    International Nuclear Information System (INIS)

    Rahman, M.; Matsouka, H.; Takami, S.; Terunuma, K.

    2001-01-01

    DTPA, DMSA and EDTA were labelled with 166 Ho of low specific activity, 250-275mCi/mg of Ho, produced from holmium oxide by the 165 Ho(n, g) 166 Ho reaction at a neutron flux of about 10 14 n.cm -2 .s -1 . Three pH ranges were selected in the study, viz. 2-3, 3-3.5 and 5-6. The labelling reactions were studied as chloride and nitrate solutions in aqueous and saline media at 30 min and 20-24 h of reaction. DTPA was labelled over 99, DMSA at about 90 and EDTA at 100.0% with 166 Ho. The complexes were found stable at all times of investigation. A beta chromatogram scanner was used to study by TLC the labelling reactions of DTPA and DMSA with the nuclide and by PC those of EDTA. A biodistribution study in three rats injected intravenous with a saline solution of 166 HoCl 3 [DTPA] at pH5.1 showed an initial uptake in blood, kidney and lung after 30 minutes. After four hours the complex was found to have cleared from blood and lung, and localized 100% in kidney. It was stable in vivo in the kidney after 24 hours. The g spectrum analysis did not show the formation of any impurity except the four characteristic g energies of 166 Ho. (author)

  17. Simultaneous Removal of Thallium and EDTA by Fenton Process

    Science.gov (United States)

    Xu, Ruibing; Huang, Xuexia; Li, Huosheng; Su, Minhua; Chen, Diyun

    2018-01-01

    The wastewater containing heavy metals and organic pollutants is widely discharged from industries. Because of the coexistence of heavy metals and organic pollutants, the treatment of such wastewater is very difficult. Fenton process is considered to be one of the most effective approaches for the degradation of organic pollutants in aqueous solution due to the strong oxidative ability of hydroxyl radical which generated from the Fenton process. Apart from this, heavy metals are able to be removed during Fenton process owning to the synergic effect of coagulation and precipitation. In this work, pollutants of thallium and EDTA were successfully removed via the Fenton process. A series of single-factor experiments were designed and performed to achieve an optimal reaction conditions for the removal of both thallium and EDTA. Results showed that the removal efficiencies of thallium and TOC could be as high as 96.54% and 70.42%, respectively. The outcomes from our study demonstrate that Fenton process is a promising method for the purification of wastewater containing thallium and EDTA.

  18. Mobilisation of heavy metals into the urine by CaEDTA: relation to erythrocyte and plasma concentrations and exposure indicators.

    Science.gov (United States)

    Araki, S; Aono, H; Murata, K

    1986-09-01

    To investigate the effects of calcium disodium ethylenediamine tetra-acetate (CaEDTA) on the urinary excretion, erythrocyte, and plasma concentrations and exposure indicators of seven heavy metals, CaEDTA was administered by intravenous infusion to 20 workers exposed to lead, zinc, and copper. The workers' blood lead concentrations ranged from 22 to 59 micrograms/dl (mean 38 micrograms/dl (1.8 mumol/l]. The 24 hour urinary excretion of metals after CaEDTA administration (mobilisation yield) was on average 13 times the background excretion for lead, 11 times for zinc, 3.8 times for manganese, 3.4 times for cadmium, 1.3 times for copper, and 1.1 times for chromium; no significant increase was found for mercury. The mobilisation yield of lead (MPb) was significantly correlated with whole blood and erythrocyte concentrations and the urinary excretion of lead but not with its plasma concentration; similarly, the mobilisation yield of cadmium was significantly correlated with its erythrocyte concentration. In addition, MPb was significantly correlated with intra-erythrocytic enzyme delta-aminolaevulinic acid dehydratase activity and urinary coproporphyrin excretion. The relation between the mobilisation yield of heavy metals and their body burden (and toxic signs) is discussed in the light of these findings.

  19. Experimental Determination of Lead Interactions with Citrate and EDTA in NaCl and MgCl2 Solutions to High Ionic Strength and Its Applications.

    Energy Technology Data Exchange (ETDEWEB)

    Xiong, Yongliang [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States). Carlsbad Programs Group; Kirkes, Leslie Dawn [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States). Carlsbad Programs Group; Westfall, Terry [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States). Carlsbad Programs Group; Marrs, Cassandra [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States). Carlsbad Programs Group; Knox, Jandi [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States). Carlsbad Programs Group; Burton, Heather Lynn [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States). Carlsbad Programs Group

    2017-09-01

    For this study, the interactions of lead with citrate and ethylenediaminetetraacetate (EDTA) are investigated based on solubility measurements as a function of ionic strength at room temperature (22.5 ± 0.5°C) in NaCl and MgCl2 solutions. The formation constants (log β10 ) for Pb[C3H5O(COO)3]– (abbreviated as PbCitrate) and Pb[(CH2COO)2N(CH2)2N(CH2COO)2)]2– (abbreviated as PbEDTA2–) Pb2+ + [C3H5O(COO)3]3– = Pb[C3H5O(COO)3] (1) Pb2+ + (CH2COO)2N(CH2)2N(CH2COO)2)4- = Pb[(CH2COO)2N(CH2)2N(CH2COO)2)]2– (2) are evaluated as 7.28 ± 0.18 (2σ) and 20.00 ± 0.20 (2σ), respectively, with a set of Pitzer parameters describing the specific interactions in NaCl and MgCl2 media. Based on these parameters, the interactions of lead with citrate and EDTA in various low temperature environments can be accurately modelled.

  20. Effects of blanching, acidification, or addition of EDTA on vitamin C and β-carotene stability during mango purée preparation.

    Science.gov (United States)

    Guiamba, Isabel R F; Svanberg, Ulf

    2016-09-01

    The impact of acidification with citric acid, addition of EDTA or water blanching at high temperature, and short time (HTST) conducted at 90°C for 4 min, on the retention of vitamin C (L-AA and DHAA) and β-carotene was studied in mango purée 30 min after crushing. HTST blanching prior to matrix disruption into purée resulted in complete inactivation of polyphenol oxidase (PPO) and minor residual activity (8%) of ascorbic acid oxidase (AAO). The retention of total vitamin C was 100% in blanched purées and in purée with EDTA and about 90% in purées at pH 3.9 and 5.0. Acidification, blanching, and addition of EDTA preserved vitamin C mainly as L-AA, while complete conversion into DHAA was observed in purée at pH 5.0. The retention of all-trans-β-carotene was between 65 and 72%, with the highest value in purée with EDTA and the lowest value in purée of blanched mango. The ratio of 13-cis-β-carotene in fresh mango was 8.2 ± 0.5% that increased significantly after blanching and in purée at pH 5.0.

  1. Evaluating the antimicrobial activity of Nisin, Lysozyme and Ethylenediaminetetraacetate incorporated in starch based active food packaging film.

    Science.gov (United States)

    Bhatia, Sugandha; Bharti, Anoop

    2015-06-01

    The pleothera of micro organisms obtained from contaminated food cultured in a starch broth was effectively tested against antibacterial agents, i.e. nisin, lysozyme and chelating agent EDTA. A variety of combination treatments of these antimicrobial agents and their incorporation in Starch based active packaging film according to their permissibility standards was done. 4 variables of Nisin concentration (ranging from 0 to 750 IU/ml), 3 variables of lysozyme concentration (ranging from 0 to 500 IU/ml) and 3 variables of EDTA concentration from (0 to 20 μM) were chosen. Bacterial inhibition by combination of different levels of different factors without antimicrobial films was evaluated using a liquid incubation method. The samples were assayed for turbidity at interval of 2, 4 and 24 h to check effectiveness of combined effects of antimicrobial agents which proved a transitory bactericidal effect for short incubation times. Zone of Inhibition was observed in the antimicrobial films prepared by agar diffusion method. Statistical analysis of experimental data for their antimicrobial spectrum was carried out by multi regression analysis and ANOVA using Design-Expert software to plot the final equation in terms of coded factors as antimicrobial agents. The experimental data indicated that the model was highly significant. Results were also evaluated graphically using response surface showing interactions between two factors, keeping other factor fixed at values at the center of domain. Synergy was also determined among antibacterial agents using the fractional inhibitory concentration (FIC) index which was observed to be 0.56 supporting the hypothesis that nisin and EDTA function as partial synergistically. The presented work aimed to screen in quick fashion the combinatorial effect of three antimicrobial agents and evaluating their efficacy in anti microbial film development.

  2. [Evaluation of initial results of treatment of lead poisoning with EDTA].

    Science.gov (United States)

    Petkova, V; Adjarov, D; Pavlova, S; Naydenova, E; Kerimova, M; Kuneva, T

    1994-01-01

    The results of EDTA therapy were studied in 37 workers of a battery factory consisting of males with varying degrees of occupational lead poisoning (low exposure: 10 subjects, blood lead levels (PbB) lower than 400 micrograms/l with slight alterations in heme biosynthesis; beyond limit of effect: 5 subjects, PbB > 400 micrograms/l; slight intoxication: 19 subjects, with marked alterations in heme synthesis and preclinical signs of intoxication; average degree of intoxication: 3 subjects with clinical signs of intoxication. Clinical symptoms and the following parameters were investigated: blood lead (PbB), delta-aminolevulinic acid dehydratase in erythrocytes (ALA-D), zinc protoporphyrin (PP) in erythrocytes and delta-aminolevulinic acid (ALA) in 24-hour urine before and after EDTA chelating therapy. Simultaneous measurement of ALA-D and PP showed high diagnostic sensitivity in detecting lead poisoning in occupationally exposed subjects. In view of the high interindividual variability of the results, these indices did not, however, permit a useful differentiation to be made of the different degrees of intoxication at individual level, even though a good correlation was observed between PbB and porphyrin metabolism indices. From the alterations observed in ALA-D and PP values it was not possible to establish an association between degree of alteration and types of clinical symptoms in the different intoxication studies. At the end of EDTA treatment, a clinical improvement was observed in all cases studied but only in 5 cases was a reduction in PbB observed, to levels below 1.20 mol/l, which is accepted as a permissible limit for the general population; in 17 cases PbB remained at levels above the critical value for occupational lead poisoning (400 micrograms/l), although there was a decrease after treatment. The improvement observed in the indices of porphyrin metabolism at the end of treatment was only slight: significant variations were measured only for PbB. After

  3. Kinetics of some rare earth ions substitution for neodymium(3) ion in aqueous solutions of ethylenediaminetetraacetate

    International Nuclear Information System (INIS)

    Nikitenko, S.I.; Martynenko, L.I.; Afonin, E.G.; Pechurova, N.I.

    1984-01-01

    Kinetics of electrophilic interaction in the systems NdA - -Ln 3+ (where Ln 3+ - Tu 3+ , Er 3+ , Gd 3+ , Eu 3+ , Pr 3+ ) has been studied. It is found that the following kinetic equation: W=W 1 +W 2 = (Ksub(1)csub(Hsup(+))+Ksub(2)csub(Lnsup(3+) 3+))csub(NdAsup(-)), where csub(Lnsup(3+)) and csub(NdA - ) - initial concentrations of Ln 3+ and NdA - , corresponds to the exchange rate. It is shown that W 1 - component of exchange rate according to the mechanism of acid dissociation, and W 2 - according to associative mechanism. The values of K 1 and K 2 at t=20 deg C, μ=0.1 are calculated. The dependence of K 2 on the nature of the ligand included is studied. It is shown that K 2 depends linearly on stability constant of Ln 3+ iminodiacetate

  4. Kinetics of some rare earth ions substitution for neodymium(3) ion in aqueous solutions of ethylenediaminetetraacetate

    Energy Technology Data Exchange (ETDEWEB)

    Nikitenko, S.I.; Martynenko, L.I.; Afonin, E.G.; Pechurova, N.I. (Moskovskij Gosudarstvennyj Univ. (USSR))

    1984-04-01

    Kinetics of electrophilic interaction in the systems NdA/sup -/-Ln/sup 3 +/ (where Ln/sup 3 +/ - Tm/sup 3 +/, Er/sup 3 +/, Gd/sup 3 +/, Eu/sup 3 +/, Pr/sup 3 +/) has been studied. It is found that the following kinetic equation: W=W/sub 1/+W/sub 2/ = (Ksub(1)csub(Hsup(+))+Ksub(2)csub(Lnsup(3+) 3+))csub(NdAsup(-)), where csub(Lnsup(3+)) and csub(NdA/sup -/) - initial concentrations of Ln/sup 3 +/ and NdA/sup -/, corresponds to the exchange rate. It is shown that W/sub 1/ - component of exchange rate according to the mechanism of acid dissociation, and W/sub 2/ - according to associative mechanism. The values of K/sub 1/ and K/sub 2/ at t=20 deg C, ..mu..=0.1 are calculated. The dependence of K/sub 2/ on the nature of the ligand included is studied. It is shown that K/sub 2/ depends linearly on stability constant of Ln/sup 3 +/ iminodiacetate.

  5. The effect of media composition on EDTA degradation by Agrobacterium sp

    International Nuclear Information System (INIS)

    Palumbo, A.V.; Lee, S.Y.; Boerman, P.

    1994-01-01

    EDTA degradation by an Agrobacterium sp. has been examined by quantifying 14 C-labeled CO 2 produced from iron-[2- 14 C] EDTA and by measured loss of nonlabeled EDTA by HPLC. Fe-EDTA degradation resulted in a rise in pH, nitrate concentration, and ammonia concentration. Addition of glycerol resulted in suppression of Fe-EDTA degradation and in a decrease in pH and NH 4 + concentration in the media. Addition of peptone or yeast extract did not affect degradation. Some of the components (e.g., biotin) of the media are not necessary for growth and biodegradation. Although cobalt-EDTA cannot be degraded, ferrous iron can be added to displace the cobalt. The purpose was to relate the results to remediation of radionuclide-contaminated soils. 12 refs., 4 figs., 2 tabs

  6. Oxidative dehydrogenation of the 2-aminomethylpyridine (EDTA) ruthenium (III) complex

    International Nuclear Information System (INIS)

    Toma, H.E.; Tsurumaki, M.

    1990-01-01

    The oxidative dehydrogenation of the 2-aminomethylpyridine (ampy) ligand coordinated to the (EDTA)RU(III) complex was investigated based on cyclic voltammetry, spectoelectrochemistry and stopped-flow kinetic measurements in aqueous solution. The reaction mechanism is consistent with the deprotonation of the ampy ligand (pk a =7.48), followed by a reversible one-electron transfer step. The intermediate species generated at this step undergoes a metal-induced electron transfer process, with k=227 s -1 , converting into the corresponding 2-iminomethylpyridine complex. (author) [pt

  7. [51Cr]EDTA plasma clearance and endogenous creatinine clearance in advanced renal insufficiency

    International Nuclear Information System (INIS)

    Svendsen, U.G.; Munck, O.; Czartoryski, A.; Stafanger, G.

    1978-01-01

    Comparison of [ 51 Cr]EDTA plasma clearance corrected for extrarenal elimination with 24 h endogenous creatinine clearance in patients with advanced renal failure showed that the corrected [ 51 Cr]EDTA clearance was lower than creatinine clearance, and thus might be a better approximation to the glomerular filtration rate in uraemic patients. The corrections cannot be used on [ 51 Cr]EDTA clearance values below the mean extrarenal clearance, averaging 3.7 ml/min. (Auth.)

  8. In vitro comparison of different 24% EDTA gel formulations efficacy on root surface conditioning

    OpenAIRE

    Sousa, Cliciane Portela; Frizzera, Fausto; Batista, Luiz Henrique Carvalho; Dantas, Andrea Abi Rached; Zandim-Barcelos, Daniela Leal; Sampaio, José Eduardo Cezar

    2013-01-01

    INTRODUCTION: The main goal of root biomodification is to modify the root surface in order to improve the repair of periodontal tissues destroyed by periodontal disease. OBJECTIVE: To evaluate the conditioning efficacy of 24% EDTA gel of different trademarks, considering the variables time and application method, by scanning electron microscopy. MATERIAL AND METHOD: 225 samples were randomly assigned to five groups: sterile saline solution (control); 24% EDTA (Santa Paula Pharmacy); 24% EDTA-...

  9. Teratogenic effect of calcium edetate (CaEDTA) in rats and the protective effect of zinc.

    Science.gov (United States)

    Brownie, C F; Brownie, C; Noden, D; Krook, L; Haluska, M; Aronson, A L

    1986-03-15

    The calcium chelate of EDTA (CaEDTA) currently is the drug of choice in the treatment of lead intoxication. This study investigated the teratogenic potential of CaEDTA, administered parenterally during periods of organogenesis and determined if incorporating zinc into EDTA would protect against teratogenic effects. Four doses (2, 4, 6, and 8 mmol/m2/day) of CaEDTA, two concentrations (8 and 20 mmol/m2/day) of ZnEDTA and ZnCaEDTA (molar ratio 0.5:0.5:1) were used, and a saline control (0.9% NaCl). Timed-pregnant Long-Evans rats were assigned at random to the treatment groups, 20 per dose for each chelate and 30 to the saline control. Rats were injected with the chelate or saline solution sc, twice daily during the 11th through 15th days of gestation. Pups removed by cesarean section on the 21st day were processed for osseous and visceral examination. Additional animals per treatment group were used for maternal plasma and liver and fetal zinc determinations. Results showed increases in several abnormalities (submucous cleft, cleft palate, adactyly-syndactyly, curly tail, abnormal rib and vertebrae) with increasing amounts of CaEDTA. No malformations were seen with ZnEDTA at either dose or with ZnCaEDTA at 8 mmol/m2/day. However, submucous cleft was seen in 6 of 20 litters from the dams receiving the higher dose of ZnCaEDTA. It was concluded that CaEDTA is teratogenic in rats at concentrations which, except for decreased weight gain, produce no discernible toxicity to the dam, and which are comparable to the recommended therapeutic dosage in humans (1500 mg/m2/day corresponding to 4 mmol/m2/day). Protection is afforded by incorporating zinc in the chelate.

  10. Comparison between high concentration EDTA (24%) and low concentration EDTA (3%) with surfactant upon removal of smear layer after rotary instrumentation: a SEM study.

    Science.gov (United States)

    Daghustani, Mohsen; Alhammadi, Ahmad; Merdad, Khalid; Ohlin, Johan; Erhardt, Fredrik; Ahlquist, Michael

    2011-01-01

    This in vitro study compare cleanliness of tooth canal walls regarding smear layer after final treatment with 24% ethylenediaminetetraaceticacid (EDTA) and 3% EDTA with or without surfactant. Sixty extracted teeth, randomly distributed into four groups, were prepared using ProFile instruments (DENTSPLY, Maillefer, Ballaigues, Switzerland), and subjected to different final irrigation solutions: group A, 24% EDTA; group B, 3% EDTA with surfactant; group C (positive control), 3% EDTA; and group D (negative control), 0.5% sodium hypochlorite. Roots were sectioned, examined and evaluated under scanning electron microscope; microphotographs were taken for the coronal, middle and apical third of each specimen. Statistical analysis showed no difference regarding presence of smear layer between test groups in the coronal and apical sections. They were cleaned in the coronal sections and uncleaned in the apical sections. In the middle section, group B was significantly cleaner (p EDTA did not improve root canal cleanliness and there is no difference between different EDTA concentrations in removing the smear layer.

  11. Tuning the EDTA-induced self-assembly and plasmonic spectral properties of gold nanorods: application in surface-enhanced Raman scattering

    Energy Technology Data Exchange (ETDEWEB)

    Li, Jian-jun [Xi’an Jiaotong University, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology (China); Zhang, Ning; Wang, Jingyuan [The First Affiliated Hospital of Xi’an Jiaotong University, Department of Clinical Laboratory (China); Yang, Chun-yu; Zhu, Jian, E-mail: nanoptzj@163.com; Zhao, Jun-wu, E-mail: nanoptzhao@163.com [Xi’an Jiaotong University, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology (China)

    2016-02-15

    Self-assembly of cetyl trimethyl ammonium bromide-protected colloidal gold nanorods with different aspect ratios has been studied by adding the ethylene diamine tetraacetic acid (EDTA). Both the assembly strength and assembly configuration fashion of the gold nanorods could be tuned by changing the aspect ratio. For gold nanorods with small aspect ratio, side-by-side assembly takes the major role in the aggregation. In this case, the blue shift of the longitudinal absorption and the increase of the transverse absorption lead to the great uplift of the middle spectrum dip as the EDTA is increased. For gold nanorods with large aspect ratio, end-to-end assembly takes the major role in the aggregation. In this case, the longitudinal absorption peak fades down rapidly and a tailing absorption peak at longer wavelength uplifts greatly as the EDTA is increased. The surface-enhanced Raman scattering (SERS) activity of the assembled gold nanorods has been studied using alpha-fetoprotein (AFP) as the Raman active probe. It has been found that both the side-by-side assembly and end-to-end assembly of the gold nanorods could effectively improve the Raman signal of the AFP. And the gold nanorod substrate with side-by-side assembly has higher SERS activity. Graphical Abstract: Side-by-side assembly of gold nanorods leads to the middle spectrum dip of LSPR uplift greatly as the EDTA is increased, which also effectively improves the SERS activity.

  12. Tuning the EDTA-induced self-assembly and plasmonic spectral properties of gold nanorods: application in surface-enhanced Raman scattering

    International Nuclear Information System (INIS)

    Li, Jian-jun; Zhang, Ning; Wang, Jingyuan; Yang, Chun-yu; Zhu, Jian; Zhao, Jun-wu

    2016-01-01

    Self-assembly of cetyl trimethyl ammonium bromide-protected colloidal gold nanorods with different aspect ratios has been studied by adding the ethylene diamine tetraacetic acid (EDTA). Both the assembly strength and assembly configuration fashion of the gold nanorods could be tuned by changing the aspect ratio. For gold nanorods with small aspect ratio, side-by-side assembly takes the major role in the aggregation. In this case, the blue shift of the longitudinal absorption and the increase of the transverse absorption lead to the great uplift of the middle spectrum dip as the EDTA is increased. For gold nanorods with large aspect ratio, end-to-end assembly takes the major role in the aggregation. In this case, the longitudinal absorption peak fades down rapidly and a tailing absorption peak at longer wavelength uplifts greatly as the EDTA is increased. The surface-enhanced Raman scattering (SERS) activity of the assembled gold nanorods has been studied using alpha-fetoprotein (AFP) as the Raman active probe. It has been found that both the side-by-side assembly and end-to-end assembly of the gold nanorods could effectively improve the Raman signal of the AFP. And the gold nanorod substrate with side-by-side assembly has higher SERS activity. Graphical Abstract: Side-by-side assembly of gold nanorods leads to the middle spectrum dip of LSPR uplift greatly as the EDTA is increased, which also effectively improves the SERS activity

  13. The effects of CaEDTA injection on lead, zinc, copper and ALAD in erythrocyte, plasma and urine in lead-exposed workers: a 24-h observation.

    Science.gov (United States)

    Aono, H; Araki, S

    1984-01-01

    To evaluate the effects of calcium disodium ethylenediamine tetraacetate (CaEDTA) on the concentrations of lead, zinc and copper in plasma, erythrocyte and urine, and the delta-aminolevulinic acid dehydratase (ALAD) activity in erythrocyte, we administered CaEDTA in 1-h intravenous infusion to ten male gun metal founders with blood-lead concentration of 39 to 64 micrograms/dl (mean 49 micrograms/dl). We found that the plasma concentration of lead, following a rapid rise within the first 3 h, fell temporarily to the level significantly lower than the initial level 19 h after start of the infusion. The plasma concentration of zinc fell to the minimal level 5 h after the infusion; and the erythrocyte concentration of zinc and the ALAD activity concurrently rose to the maximal level 5 h after the infusion. By contrast, no significant alteration was observed in the concentrations of copper in plasma and erythrocyte. The maximal level of urinary metal excretion was attained during the period between 1 and 2 h after start of CaEDTA infusion for lead; within 2 h for zinc; and between 2 and 4 h for copper. The urinary metal excretion returned to the initial level 14 to 24 h after infusion for zinc and copper; but lead excretion was still higher than the initial level during this period. The difference in the kinetics of the three metals following CaEDTA injection is discussed in the light of these findings.

  14. Effects of CaEDTA injection on lead, zinc, copper and ALAD in erythrocyte, plasma and urine in lead-exposed workers: a 24-h observation

    Energy Technology Data Exchange (ETDEWEB)

    Aono, H.; Araki, S.

    1984-01-01

    To evaluate the effects of calcium disodium ethylenediamine tetraacetate (CaEDTA) on the concentrations of lead, zinc and copper in plasma, erythrocyte and urine, and the delta-aminolevulinic acid dehydratase (ALAD) activity in erythrocyte, we administered CaEDTA in 1-h intravenous infusion to ten male gun metal founders with blood-lead concentration of 39 to 64 micrograms/dl (mean 49 micrograms/dl). We found that the plasma concentration of lead, following a rapid rise within the first 3 h, fell temporarily to the level significantly lower than the initial level 19 h after start of the infusion. The plasma concentration of zinc fell to the minimal level 5 h after the infusion; and the erythrocyte concentration of zinc and the ALAD activity concurrently rose to the maximal level 5 h after the infusion. By contrast, no significant alteration was observed in the concentrations of copper in plasma and erythrocyte. The maximal level of urinary metal excretion was attained during the period between 1 and 2 h after start of CaEDTA infusion for lead; within 2 h for zinc; and between 2 and 4 h for copper. The urinary metal excretion returned to the initial level 14 to 24 h after infusion for zinc and copper; but lead excretion was still higher than the initial level during this period. The difference in the kinetics of the three metals following CaEDTA injection is discussed in the light of these findings.

  15. Effects of Application of NTA and EDTA on Accumulation of Soil Heavy Metals in Chrysanthemum

    Science.gov (United States)

    Bai, Weiyang

    2018-02-01

    In order to find out the effect of non-bio chelating agent EDTA and bio-chelating agent NTA on soil heavy metal pollution, the effects of different ratio of chelating agent NTA and EDTA on soil heavy metals (Pb, Cu and Cd ), the effects of chelating on content of chlorophyll and vitamin C and the degree of soil nutrient loss were evaluated. The results showed: that the contents of Pb and Cd were the highest in the roots of Chrysanthemum in the treatment (EDTA / NTA = 2: 1). The treatment (EDTA / NTA = 1: 1) was the best chelating agent ratio for the synergistic effect, which can significantly promoted the Chrysanthemum on heavy metal Cu uptake and transport to aboveground. Chrysanthemum were inhibited by all chelating agents treatments, while the content of chlorophyll and vitamin C of the Chrysanthemum physiological indexes were reduced. In the treatment (EDTA = 1), chlorophyll SPAD, vitamin C content reached a minimum of 36 and 38mg · 100g-1, respectively. The nutrient element TN in the leachate were gradually decreased with the time, and the tenth day was significantly lower than the leaching rate of the first day (p treatment (EDTA = 1) >treatment (EDTA / NTA = 2: 1) >treatment (EDTA / NTA = 1: 2)> treatment (NTA = 1). Treatment (EDTA / NTA = 2: 1) was recommended for the chelating agent ratio with the better physiological parameters, the more heavy metal extraction and the less nitrogen and phosphorus loss

  16. Ion chromatographic determination of vanadium (4) and vanadium (5) in the form of EDTA complexes

    International Nuclear Information System (INIS)

    Komarova, T.V.; Obrezkov, O.N.; Shpigun, O.A.

    1991-01-01

    Ion chromatographic behaviour of V 4+ and V 5+ -EDTA complexes (1,2) and V 5+ -EDTA monoperoxo complexes (3) has been studied. The regularities of ion exchange are obeyed for 1 and 3 complexes. These complex anions are strongly retained on HIX-1 anion exchanger. The chromatographic characteristics of 2 change in time, therefore its determination is impossible under the conditions studied. A method has been developed of the simultaneous determination of V 4+ and V 5+ which is based on retarded formation of the V 5+ -EDTA monoperoxo complex in a system of V 4+ -EDTA - H 2 O 2

  17. Electrochemical EDTA recycling with sacrificial Al anode for remediation of Pb contaminated soil

    International Nuclear Information System (INIS)

    Pociecha, Maja; Lestan, Domen

    2010-01-01

    Recycling chelant is a precondition for cost-effective EDTA-based soil remediation. Extraction with EDTA removed 67.5% of Pb from the contaminated soil and yielded washing solution with 1535 mg L -1 Pb and 33.4 mM EDTA. Electrochemical treatment of the washing solution using Al anode, current density 96 mA cm -2 and pH 10 removed 90% of Pb from the solution (by electrodeposition on the stainless steel cathode) while the concentration of EDTA in the treated solution remained the same. The obtained data indicate that the Pb in the EDTA complex was replaced by electro-corroded Al after electro-reduction of the EDTA and subsequently removed from the solution. Additional soil extraction with the treated washing solution resulted in total removal of 87% of Pb from the contaminated soil. The recycled EDTA retained the Pb extraction potential through several steps of soil extraction and washing solution treatment, although part of the EDTA was lost by soil absorption. - Aluminium anode at alkaline pH in conventional electrolytic cell enables efficient recycling of EDTA as a part of soil washing remediation technology.

  18. Efeito do EDTA e EDTA-T aplicado de forma ativa na remoção de "smear layer" de superfícies radiculares

    OpenAIRE

    Sampaio, José Eduardo Cezar; Rached, Ricardo Samih Georges Abi; Pilatti, Gibson Luiz; Theodoro, Letícia Helena; Batista, Luiz Henrique Carvalho

    2003-01-01

    The purpose of this study was to compare the removal of root surface smear layer following active application of EDTA gel and EDTA-T (texapon) gel in different concentrations (5%, 10%, 15%, 20% and 24%), using scanning electron microscopy. A total of 220 dentin blocks obtained from the root surfaces of extracted teeth were divided into 3 groups: Group I - (control) application of saline solution (n = 20); Group II - EDTA gel (pH 7.0) was applied in the following concentrations: 5%, 10%, 15%, ...

  19. Impacts of EDTA on uptake and accumulation of Cu 2+ by spinach ...

    African Journals Online (AJOL)

    The effects of EDTA on Cu2+ uptake by spinach (spinacia oleracea L) seedlings replanted in hydroponic solutions in a greenhouse were investigated. Four week old seedlings were exposed to various doses of Cu2+ (0, 5, 10, 15, and 20mg/L) and constant concentration of EDTA (10mM). During the exposure, the plant ...

  20. Chemodynamics of EDTA in a simulated mixed waste: the Hanford Site's complex concentrate waste

    International Nuclear Information System (INIS)

    Toste, A.P.; Ohnuki, Toshihiko

    1999-01-01

    Enormous stockpiles of mixed wastes at the USDOE's Hanford Site, the original US plutonium production facility, await permanent disposal. One mixed waste derived from reprocessing spent fuel was found to contain numerous nuclear related organics including chelating agents like EDTA and complexing agents, which have been used as decontamination agents, etc. Their presence in actual mixed wastes indicates that the organic content of nuclear wastes is dynamic and complicate waste management efforts. The subjects of this report is the chemo-degradation of EDTA degradation in a simulant Hanford's complex concentrate waste. The simulant was prepared by adding EDTA to an inorganic matrix, which was formulated based on past analyses of the actual waste. Aliquots of the EDTA simulant were withdrawn at different time points, derivatized via methylation and analyzed by gas chromatography and Gc/MS to monitor the disappearance of EDTA and the appearance of its' degradation products. This report also compares the results of EDTA's chemo-degradation to the g-radiolysis of EDTA in the simulant, the subject of a recently published article. Finally based on the results of these two studies, an assesment of the potential impact of EDTA degradation on the management of mixed wastes is offered. (J.P.N.)

  1. Using the EDTA Hole Scavenger to Accelerate Decolorization in the Immobilized Photocatalytic Process

    Directory of Open Access Journals (Sweden)

    raziye Asgari

    2015-07-01

    Full Text Available This study investigated the effect of EDTA as a hole scavenger on accelerating the photocatalytic decolorization of direct blue 71 as a non-degradable model pollutant with nano TiO2 powder immobilized on a cementitious bed. For this purpose, 75 mg/L of the dye was decolorized in 75 minutes with 0.03 M of EDTA at a pH level of 6 and under irradiation produced by a 60-W UV-C lamp. This is while decolorization under identical conditions but in the absence of EDTA had been accomplished in 225 minutes. The experiment, therefore, confirmed the accelerating effect of the scavenger on decolorization. The kinetics of the photocatalytic process with EDTA followed a first order reaction with a constant rate of 0.05 min-1, which is 2.5 times faster than the process without EDTA.

  2. Phenotypic detection of the cfiA metallo-β-lactamase in Bacteroides fragilis with the meropenem-EDTA double-ended Etest and the ROSCO KPC/MBL Confirm Kit

    DEFF Research Database (Denmark)

    Ferløv-Schwensen, Simon Andreas; Acar, Ziyap; Sydenham, Thomas V

    2017-01-01

    OBJECTIVES: To investigate the performance of the meropenem and imipenem double-ended Etest ± EDTA and the tablet-based (meropenem and meropenem + dipicolinic acid) KPC/MBL Confirm Kit to detect cfiA metallo-β-lactamase (MBL) in Bacteroides fragilis. METHODS: Well-characterized B. fragilis isolates...

  3. Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus

    Directory of Open Access Journals (Sweden)

    Thaís Heloisa Vaz Farias

    2016-05-01

    Full Text Available Abstract: The present study aimed to evaluate the effects of Na heparin and Na2EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm. Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na2EDTA 10%, Na2EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na2EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na2EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na2EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na2EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood.

  4. Characterization of EDTA-soluble polysaccharides from the scape of Musa paradisiaca (banana).

    Science.gov (United States)

    Raju, T S; Jagadish, R L; Anjaneyalu, Y V

    2001-02-01

    The polysaccharide components present in the scape of Musa paradisiaca (banana) were fractionated into water-soluble (WSP), EDTA-soluble (EDTA-SP), alkali-soluble (ASP) and alkali-insoluble (AISP) polysaccharide fractions [Anjaneyalu, Jagadish and Raju (1997) Glycoconj. J. 14, 507-512]. The EDTA-SP was further fractionated by iso-amyl alcohol into EDTA-SP-A and EDTA-SP-B. The homogeneity of these two polysaccharides was established by repeated precipitation with iso-amyl alcohol, gel-filtration chromatography and sedimentation analysis. The polysaccharides were characterized by monosaccharide composition analysis, methylation linkage analysis, iodine affinity, ferricyanide number, blue value, hydrolysis with alpha-amylase, gold-electron microscopy and X-ray diffraction spectroscopy. Data from all of these studies suggest that EDTA-SP-A is a branched amylose-type alpha-D-glucan and that EDTA-SP-B is a highly branched amylopectin-type polymer. The nature of the branching patterns of these polysaccharides suggests that they are unique to M. paradisiaca.

  5. Two supramolecular complexes based on polyoxometalates and Co-EDTA units via covalent connection or non-covalent interaction

    Energy Technology Data Exchange (ETDEWEB)

    Teng, Chunlin; Xiao, Hanxi [Key Laboratory of Theoretical Organic Chemistry and Functional Molecule for Ministry of Education, Hunan University of Science and Technology, Xiangtan 411201 (China); Cai, Qing [Chemistry Department, City University of New York, New York, NY 10016 (United States); Tang, Jianting; Cai, Tiejun [Key Laboratory of Theoretical Organic Chemistry and Functional Molecule for Ministry of Education, Hunan University of Science and Technology, Xiangtan 411201 (China); Deng, Qian, E-mail: dengqian10502@163.com [Key Laboratory of Theoretical Organic Chemistry and Functional Molecule for Ministry of Education, Hunan University of Science and Technology, Xiangtan 411201 (China)

    2016-11-15

    Two new 3D network organic-inorganic hybrid supramolecular complexes ([Na{sub 6}(CoEDTA){sub 2}(H{sub 2}O){sub 13}]·(H{sub 2}SiW{sub 12}O{sub 40})·xH{sub 2}O)n (1) and [CoH{sub 4}EDTA(H{sub 2}O)]{sub 2}(SiW{sub 12}O{sub 40})·15H{sub 2}O (2) (H{sub 4}EDTA=Ethylenediamine tetraacetic acid) have been successfully synthesized by solution method, and characterized by infrared spectrum (IR), thermogravimetric-differential thermal analysis (TG-DTA), cyclic voltammetry (CV) and single{sup −}crystal X-ray diffraction (XRD). Both of the complexes are the supramolecules, but with different liking mode, they are two representative models of supramolecule. complex (1) is a 3D infinite network supramolecular coordination polymer with a rare multi-metal sturcture of sodium-cobalt-containing, which is mainly linked through coordinate-covalent bonds. While complex (2) is normal supramolecule, which linked by non-covalent interactions, such as H-bonding interaction, electrostatic interaction and van der waals force. Both of complex (1) and (2) exhibit good catalytic activities for catalytic oxidation of methanol, when the initial concentration of methanol is 3.0 g m{sup −3}, flow rate is 10 mL min{sup −1}, and the quality of catalyst is 0.2 g, for complex (1) and complex (2) the maximum elimination rates of methanol are 85% (150 °C) and 92% (120 °C), respectively. - Graphical abstract: Two new organic-inorganic hybrid supramolecular complexes based on Co-EDTA, and Keggin polyanions have been successfully synthesized with different pH value by solution method. They are attributed to two representative models of supramolecule. Complex(1) is an infinite coordination polymer with a rare multi-metal sturcture of sodium-cobalt-containing, which is mainly linked through covalent bonds. Complex (2) is a normal supramolecule, which linked by non-covalent interactions of H-bonding interaction, electrostatic interaction and van der waals force. - Highlights: • Two supramolecules

  6. Molybdenum(6) complexing with ethylenediaminedisuccinic acid from PMR spectroscopy data

    International Nuclear Information System (INIS)

    Larchenko, V.E.; Kovaleva, I.B.; Mitrofanova, N.D.; Martynenko, L.I.

    1989-01-01

    Methods of high resolution PMR spectroscopy and pH potentiometry are used to study molybdenum(6) complexing with ethylenediaminedisuccinic acid in aqueous solutions. It is shown that molybdenum(6) interacts with ethylenediaminedisuccinic acid in the narrow range of pH values 4.0-6.5, where MoO 3 H 2 L 2 - and MoO 3 HL 3- complexes with asymmetrical structure are formed. Composition and structure of molybdenum(6) ethylenediaminedisuccinates and ethylenediaminetetraacetates are compared

  7. A Comparative Study of [CaEDTA](2-) and [MgEDTA](2-): Structural and Dynamical Insights from Quantum Mechanical Charge Field Molecular Dynamics.

    Science.gov (United States)

    Tirler, Andreas O; Hofer, Thomas S

    2015-07-09

    Structure and dynamics of [MgEDTA](2-) and [CaEDTA](2-) complexes in aqueous solution have been investigated via quantum mechanical/molecular mechanical (QM/MM) simulations. While for the first a 6-fold octahedral complex has been observed, the presence of an additional coordinating water ligand has been observed in the latter case. Because of rapidly exchanging water molecules, this 7-fold coordination complex was found to form pentagonal bipyramidal as well as capped trigonal prismatic configurations along the simulation interchanging on the picosecond time scale. Also in the case of [MgEDTA](2-) a trigonal prismatic configuration has been observed for a very short time period of approximately 1 ps. This work reports for the first time the presence of trigonal prismatic structures observed in the coordination sphere of [MgEDTA](2-) and [CaEDTA](2-) complexes in aqueous solution. In addition to the detailed characterization of structure and dynamics of the systems, the prediction of the associated infrared spectra indicates that the ion-water vibrational mode found at approximately 250 cm(-1) provides a distinctive measure to experimentally detect the presence of the coordinating water molecule via low-frequency IR setups.

  8. Phosphorus Elimination at Sodium Silicate from Quartz Sand Roasted with Complexation using Chitosan-EDTA

    Science.gov (United States)

    Wahyuningsih, S.; Ramelan, A. H.; Suharty, N. S.; Handayani, M.; Firdiyono, F.; Sulistiyono, E.; Munawaroh, H.; Sari, P. P.; Kristiawan, Y. R.

    2018-03-01

    A phosphorus elimination from sodium silicate solution has been studied. Phosphorus elimination was performed by adding chitosan-EDTA to remove cation phosphorus. Characterization of chitosan-EDTA material was performed using FT-IR, while the decreasing level of phosphorus content was analyzed by quantitative analysis using spectrophotometer UV-Vis refers to SNI 06-6989-2004. The results showed that the content of the sodium silicate can be reduced up to 67.1% through Chitosan-EDTA complexation with phosphorus.

  9. Synthesis and Characterization of EDTA Complexes Useful for Trace Elements Supplementation

    Directory of Open Access Journals (Sweden)

    Baran Enrique J.

    2002-01-01

    Full Text Available The synthesis of two heterobimetallic EDTA complexes, MgCu(EDTA.6H2O and ZnCu(EDTA.6H2O, is described. They were characterized by means of vibrational (infrared and Raman and electronic (reflectance spectroscopy. Several dissolution tests in 0.1 N HCl and simulated gastric juice were also performed. The results support the potential usefulness of these complexes for copper supplementation in human and veterinary medicine and to the simultaneous reinforcement of Mg(II and Zn(II levels.

  10. Potentiometric end point detection in the EDTA titrimetric determination of gallium

    International Nuclear Information System (INIS)

    Gopinath, N.; Renuka, M.; Aggarwal, S.K.

    2001-01-01

    Gallium is titrated in presence of known amount of Fe (III) with EDTA in HNO 3 solution at pH 2 to 3. The end point is detected potentiometrically employing a bright platinum wire - saturated calomel (SCE) reference electrode system, the redox couple being Fe (III) / Fe (II). Since Fe (III) is also titrated by EDTA, it is, therefore, subtracted from titre value to get the EDTA equivalent to gallium only. Precision and accuracy 0.2 to 0.4% was obtained in the results of gallium in the range of 8 to 2 mg. (author)

  11. An improved synthesis of (H/sub 2/EDTA)Tc(EDTAH/sub 2/) . 5H/sub 2/O

    International Nuclear Information System (INIS)

    Linder, K.E.; Davison, A.; Jones, A.G.

    1986-01-01

    A new procedure for the preparation of the dimeric technetium complex (H/sub 2/EDTA)Tc(μ-O)/sub 2/Tc(EDTAH/sub 2/)-5H/sub 2/O,/sup 1/ has been developed. In the original preparation of this complex, HTcO/sub 4/, NaHSO/sub 3/, and EDTA were heated at 70 0 C for several days and a crude product isolated by separating out crystals from a reaction mixture that had been allowed to evaporate very slowly. Analysis of the time course of this reaction shows that a reduced, presumably Tc(III), gold complex forms rapidly and that the prolonged reaction time is required only to decompose excess reducing agent and to oxidize the gold compound to the desired red product 1. The authors have found that careful oxidation of this gold complex with peroxide, followed by the addition of dilute HC1O/sub 4/, precipitates high yields of the sparingly soluble free acid form of 1 from the reaction mixture. The synthesis requires 24 hours, rather than several days

  12. Calibration of shahid's analytical method for adulterated Zn-edta fertilizers by ion chromatography and atomic absorption spectroscopy

    International Nuclear Information System (INIS)

    Khan, M.S.A.; Akram, M.; Qazi, M.A.

    2010-01-01

    Chelated zinc fertilizers are usually recommended in calcareous alkaline soils to provide Zn nutrition in order to prevent possible Zn precipitation. In Punjab (Pakistan), Zn EDTA products are being manufactured, and marketed to meet the zinc requirement of various crops grown in Zn deficient soils. Under fertilizer control order, 1973 (amended), their quality has to be monitored by the Agriculture Department, Government of the Punjab. None of the traditional method was found suitable which can separate the mineral fraction from that of chelated adulterated fertilizer except for those methods based on ion chromatography. Calibration of ion chromatography method was carried out by determining the mineral Zn fraction leading to estimate remaining Zn EDTA fraction in fertilizer samples of adulterated nature i.e. mixture of chelated and mineral fraction. In order to achieve the objective atomic absorption spectroscopy was coupled with ion chromatography. The method offers a specific, reliable technique for determination of chelated zinc in fertilizers. In the first step chelation was broken down with concentrated sulphuric acid treatment and total zinc contents were determined by atomic absorption spectroscopy. In second step, non-chelated (mineral) portion of zinc was determined by ion chromatography using cation column and conductivity detector. Chelated zinc was calculated by subtracting non-chelated (mineral) fraction from total zinc contents. (author)

  13. A scanning electron microscopy study of root surface smear layer removal after topical application of EDTA plus a detergent

    OpenAIRE

    Sampaio,José Eduardo Cezar; Campos,Flávia Pavan; Pilatti,Gibson Luiz; Theodoro,Letícia Helena; Leite,Fábio Renato Manzolli

    2005-01-01

    The aim of the present study was to compare root surface smear layer removal following topical application of EDTA and EDTA-T (Texapon). Extracted human teeth had their cementum removed and were mechanically scaled. A total of 220 root specimens were obtained and were randomly assigned to the following groups: I-saline solution (control), II-EDTA; III-EDTA-T. Groups II and III specimens were assigned to different EDTA gel concentrations: 5%, 10%, 15%, 20% and 24%. Smear layer removal score wa...

  14. Interaction of ruthenium (4) and osmium (4) with 2-mercaptobenzimidazole, 2-mercaptobenzoxazole and 2-mercaptobenzothiazole in the presence of edta

    International Nuclear Information System (INIS)

    Busev, A.I.; Ignat'eva, T.I.; Lomakina, L.N.

    1975-01-01

    Interaction of ruthenium (4) and osmium (4) with 2-mercaptobenzimidazole (2MBI), 2-mercaptobenzoxazole (2MBO) and 2-mercaptobenzothiazole (2MBT) in the presence of EDTA was studied. The interaction of ruthenium (4) and osmium (4) with EDTA was constidered. Ruthenium complex is formed with constant output at 2-4.5 pH after 30 min.heating. In the lowacid solution (pH 2-4) osmium reacts with EDTA forming soluble compound. Characteristics of ruthenium (4) compound with 2MBI, 2MBO and 2MBT produced in the presence of EDTA are presented. Osmium (4) in the presence of EDTA and above mentioned organic reagents and when heating forms lowsoluble compounds. Possibility of joint determination of ruthenium and osmium with help of 2MBI in the presence of EDTA under conditions of minimum complexing osmium with EDTA was investigated

  15. Radiometric titration of thallium(III) with EDTA

    International Nuclear Information System (INIS)

    Rao, V.R.S.; Pulla Rao, Ch.; Tataiah, G.

    1978-01-01

    Radioactive solutions containing very small amounts of thallium(III) can be determined by radiometric titration using ammonia as hydrolysing agent. Aqueous solution of thallium(I) (both inactive and radioactive) is treated with bromine water till the appearance of the brown colour of bromine, and the solution is warmed to 80 deg C to expel the excess bromine. By this procedure all thallium(I) is quantitatively oxidised to thallium(III). An aqueous solution of ammonia is added to precipitate thallium(III) as thallic oxide. It is then filtered, washed with water to free it from bromide and then dissolved in 2N HCl and the solution is then standardised. 2 ml of this solution is transferred to a 20 ml volumetric flask, 1 ml of radioactive thallium(III) solution to be standardised is added as well as incremental amounts of EDTA solution and mixed thoroughly. Uncomplexed thallium(III) is then precipitated by the addition of an ammonia solution and diluted to 20 ml. Required amount of this mixture is centrifuged. The beta activity of the supernatant aliquot is measured using a GM counter. Quantitative determination of Tl(III) in the range of 1-10 μM can be carried out. The interference of cations such as Au(III), iron(III), Ga(III) can be eliminated by pretreatment of the Tl(III) solution before carrying out radiometric titration. The results obtained are reproducible and accurate to +-3%. (T.I.)

  16. 51Cr-EDTA plasma clearance in severe renal failure determined by one plasma sample

    DEFF Research Database (Denmark)

    Kamper, A L; Nielsen, S L

    1989-01-01

    Two hundred and thirty-four measurements of standard 51Cr-EDTA plasma clearance were made in 50 patients with severe chronic renal failure. Based on these data two calculation methods were attempted using one plasma sample drawn 24 h after injection of 51Cr-EDTA. One of the methods used the 'one...... at zero-time was derived from injected dose and body surface area. This method might provide values 1.5 ml/min below or 0.8 ml/min above the established method of 51Cr-EDTA plasma clearance, which would be acceptable for clinical purposes. It is concluded that exact plasma clearance of 51Cr-EDTA in severe...... renal dysfunction (estimated clearance values below 21 ml/min) may be determined with adequate precision by one plasma sample drawn at 24 h after injection of the tracer without sampling at 5 h. This appears to be a very practical simplification....

  17. Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae

    Directory of Open Access Journals (Sweden)

    Lilian Terezinha de Queiroz Leite

    1996-10-01

    Full Text Available Monoclonal antibodies (MABs ivere produced against an etbylenediaminetetraacetate (EDTA extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgGl and IgG2b. The EDTA extract was detected as several bands by silver staining in SDS-PAGE. In the Western blot the bands around 20 KDa reacted with a monoclonal antibody, 47B4D6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohydrate nature, lmmunocytochemistry, using colloidal gold labeling, showed that an EDTA extract determinant recognized by monoclonal antibody 47B4D6, is localized under the outer envelope of serovar icterohaemorrhagiae. Hoe AIAB raised against the EDTA extract was not able to protect hamsters from lethal challenge with virulent homologous leptospires.

  18. Arabinose and ferulic acid rich pectic polysaccharides extracted from sugar beet pulp.

    NARCIS (Netherlands)

    Oosterveld, A.; Beldman, G.; Schols, H.A.; Voragen, A.G.J.

    1996-01-01

    Arabinose and ferulic acid rich polysaccharides were extracted from sugar beet pulp using two extraction methods: a sequential extraction with H2O (2 times), NaOH/EDTA (2 times), and 4 M NaOH (2 times; method A) and a sequential extraction in which the NaOH/EDTA extraction was replaced by an

  19. Late calcium EDTA rescues hippocampal CA1 neurons from global ischemia-induced death.

    Science.gov (United States)

    Calderone, Agata; Jover, Teresa; Mashiko, Toshihiro; Noh, Kyung-min; Tanaka, Hidenobu; Bennett, Michael V L; Zukin, R Suzanne

    2004-11-03

    Transient global ischemia induces a delayed rise in intracellular Zn2+, which may be mediated via glutamate receptor 2 (GluR2)-lacking AMPA receptors (AMPARs), and selective, delayed death of hippocampal CA1 neurons. The molecular mechanisms underlying Zn2+ toxicity in vivo are not well delineated. Here we show the striking finding that intraventricular injection of the high-affinity Zn2+ chelator calcium EDTA (CaEDTA) at 30 min before ischemia (early CaEDTA) or at 48-60 hr (late CaEDTA), but not 3-6 hr, after ischemia, afforded robust protection of CA1 neurons in approximately 50% (late CaEDTA) to 75% (early CaEDTA) of animals. We also show that Zn2+ acts via temporally distinct mechanisms to promote neuronal death. Early CaEDTA attenuated ischemia-induced GluR2 mRNA and protein downregulation (and, by inference, formation of Zn2+-permeable AMPARs), the delayed rise in Zn2+, and neuronal death. These findings suggest that Zn2+ acts at step(s) upstream from GluR2 gene downregulation and implicate Zn2+ in transcriptional regulation and/or GluR2 mRNA stability. Early CaEDTA also blocked mitochondrial release of cytochrome c and Smac/DIABLO (second mitochondria-derived activator of caspases/direct inhibitor of apoptosis protein-binding protein with low pI), caspase-3 activity (but not procaspase-3 cleavage), p75NTR induction, and DNA fragmentation. These findings indicate that CaEDTA preserves the functional integrity of the mitochondrial outer membrane and arrests the caspase death cascade. Late injection of CaEDTA at a time when GluR2 is downregulated and caspase is activated inhibited the delayed rise in Zn2+, p75NTR induction, DNA fragmentation, and cell death. The finding of neuroprotection by late CaEDTA administration has striking implications for intervention in the delayed neuronal death associated with global ischemia.

  20. Potentiometric determination of sulfate with EDTA and the cupric-selective electrode

    International Nuclear Information System (INIS)

    Baumann, E.W.

    1976-11-01

    Sulfate was indirectly determined by precipitating sulfate as BaSO 4 and then dissolving BaSO 4 in excess ammoniacal EDTA. The excess EDTA was titrated potentiometrically with La 3+ . A cupric-selective electrode was used to detect the end point. About 10 -3 M SO 4 2- was determined in 3M HCl solutions of metal oxides with a relative standard deviation of 3.5 percent and a bias of +4 percent

  1. Effects of Na2CaEDTA on lead deposits in rabbit osseous tissue

    International Nuclear Information System (INIS)

    Doniec, J.; Trojanowska, B.; Trzcinka-Ochocka, M.; Garlicka, I.

    1983-01-01

    Radiochemical and autoradiographic methods were used for 210 Pb determination after Na 2 CaEDTA administration to rabbits. 210 Pb was determined in soft tissues, compact and trabecular bones and growing microareas on the endosteum of the long bone. After Na 2 CaEDTA injection lead was depleted from 'new' deposits mainly on the growing surface of both trabecular and compact bones. (Auth.)

  2. First-Row-Transition Ion Metals(II-EDTA Functionalized Magnetic Nanoparticles as Catalysts for Solvent-Free Microwave-Induced Oxidation of Alcohols

    Directory of Open Access Journals (Sweden)

    Nuno M. R. Martins

    2017-11-01

    Full Text Available A series of first-row transition-metals combined with ethylenediamine tetraacetic acid (EDTA, as metal-based N,O-chelating ligands, at the surface of ferrite magnetic nanoparticles (MNPs was prepared by a co-precipitation method. Those EDTA functionalized MNPs with general formula Fe3O4@EDTA-M2+ [M = Mn2+ (1, Fe2+ (2, Co2+ (3, Ni2+ (4, Cu2+ (5 or Zn2+ (6] were characterized by FTIR (Fourier Transform Infrared spectroscopy, powder XRD (X-ray Diffraction, SEM (Scanning Electron Microscope, EDS (Energy Dispersive Spectrometer, VSM (Vibrating Sample Magnetometer and TGA (Thermal Gravity Analysis. The application of the magnetic NPs towards the microwave-assisted oxidation of several alcohol substrates in a solvent-free medium was evaluated. The influence of reaction parameters such as temperature, time, type of oxidant, and presence of organic radicals was investigated. This study demonstrates that these MNPs can act as efficient catalysts for the conversion of alcohols to the corresponding ketones or aldehydes with high selectivity and yields up to 99% after 2 h of reaction at 110 °C using t-BuOOH as oxidant. Moreover, they have the advantage of being magnetically recoverable catalysts that can be easily recycled in following runs.

  3. Fluorescence turn-on sensing of trace cadmium ions based on EDTA-etched CdTe@CdS quantum dot.

    Science.gov (United States)

    Wang, Si-Nan; Zhu, Jian; Li, Xin; Li, Jian-Jun; Zhao, Jun-Wu

    2018-05-01

    Cadmium-caused environmental pollution and diseases have always been worldwide problems. Thus it is extremely urgent to establish a cheap, rapid, simple and selective detection method for trace cadmium in drinking water. In this study, a fluorescence "turn-on" method based on ethylene diamine tetraacetic acid (EDTA)-etched CdTe@CdS quantum dots (QDs) was designed to detect Cd 2+ . High resolution transmission electron microscopy (HRTEM) and X-ray photoelectron spectroscopy (XPS) were utilized for chemical and structural characterization of the as-prepared QDs. Based on chemical etching of EDTA on the surface of CdTe@CdS QDs, specific Cd 2+ recognition sites were produced, and then results in fluorescence quenching. The introduction of Cd 2+ could identify these sites and restore the fluorescence of the EDTA-QDs system. Under the optimum conditions, the nanoprobe shows a linear response range from 0.05 to 9 μM with a very low detection limit of 0.032 μM. In addition, the reported fluorescence probe in this work displays a good selectivity for trace Cd 2+ over other metal ions and an admirable practicability in real water samples. Copyright © 2018 Elsevier B.V. All rights reserved.

  4. EDTA treatment alters protein glycosylation in the cellular slime mold Dictyostelium discoideum

    International Nuclear Information System (INIS)

    West, C.M.; Brownstein, S.A.

    1988-01-01

    The authors have found that treatment of cells with EDTA resulted in the accumulation of lower molecular weight forms of two cell-type-specific glycoproteins. These new glycoproteins lacked a developmentally regulated glycoantigen defined by monoclonal antibody 54.2. Since EDTA dissociated the cells, the possible involvement of cell separation was tested by immobilizing cells in soft agarose. Glycoantigen expression on these proteins was found to be dependent on cAMP and high oxygen tension but not on cell contact, and was reversibly sensitive to EDTA regardless of the state of cell association. The EDTA effect was mimicked by other soluble, but not particulate, membrane impermeable chelators, could be completed by Zn 2+ better than Mg 2+ , and appeared to involve an intracellular mechanism. Studies with [ 14 C]EDTA showed that EDTA equilibrated with a cellular compartment in a temperature-dependent, Zn 2+ -insensitive fashion with half-time kinetics of loading and unloading of 30-40 min. The data suggest that this step in glycosylation, which was found to be delayed 1 or more hours subsequent to protein synthesis, involves an intracellular, transition metal-ion-dependent process which can be modulated by chelators entering the cell through the endocytic pathway

  5. Effect of NaFeEDTA-fortified soy sauce on zinc absorption in children.

    Science.gov (United States)

    Li, Min; Wu, Jinghuan; Ren, Tongxiang; Wang, Rui; Li, Weidong; Piao, Jianhua; Wang, Jun; Yang, Xiaoguang

    2015-03-01

    NaFeEDTA has been applied in many foods as an iron fortificant and is used to prevent iron deficiency in Fe-depleted populations. In China, soy sauce is fortified with NaFeEDTA to control iron deficiency. However, it is unclear whether Fe-fortified soy sauce affects zinc absorption. To investigate whether NaFeEDTA-fortified soy sauce affects zinc absorption in children, sixty children were enrolled in this study and randomly assigned to three groups (10 male children and 10 female children in each group). All children received daily 3 mg of (67)Zn and 1.2 mg of dysprosium orally, while the children in the three groups were supplemented with NaFeEDTA-fortified soy sauce (6 mg Fe, NaFeEDTA group), FeSO₄-fortified soy sauce (6 mg Fe, FeSO₄ group), and no iron-fortified soy sauce (control group), respectively. Fecal samples were collected during the experimental period and analyzed for the Zn content, (67)Zn isotope ratio and dysprosium content. The Fe intake from NaFeEDTA-fortified and FeSO₄-fortified groups was significantly higher than that in the control group (P sauce does not affect Zn bioavailability in children.

  6. Enhanced degradation of 4-nitrophenol by microwave assisted Fe/EDTA process

    Energy Technology Data Exchange (ETDEWEB)

    Liu Bo, E-mail: yongboliu@163.com [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Li Song, E-mail: ls8214@163.com [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Zhao Yongjun, E-mail: zyjun2007@126.com [State Key Laboratory of Pollution Control and Resource Reuse, School of Life Science, Nanjing University, Nanjing 210093 (China); Wu Wenfei, E-mail: feibanana6@163.com [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Zhang Xuxiang, E-mail: zhangxx@nju.edu.cn [State Key Laboratory of Pollution Control and Resource Reuse, School of Life Science, Nanjing University, Nanjing 210093 (China); Gu Xueyuan, E-mail: xygu@nju.edu.cn [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Li Ruihua, E-mail: liruihua@nju.edu.cn [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Yang Shaogui, E-mail: ysg420@sina.com [State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China)

    2010-04-15

    A microwave assisted zero-valent iron oxidation process was studied in order to investigate the synergetic effects of MW irradiation on Fe/EDTA system (Fe/EDTA/MW) treated 4-nitrophenol (4-NP) from aqueous solution. The results indicated that the thermal effect of microwave improved the removal effect of 4-NP and TOC through raising the temperature of the system, as well as the non-thermal effect generated by the interaction between the microwave and the Fe resulting in an increase in the hydrophobic character of Fe surface. During the degradation of 4-NP in Fe/EDTA/MW system, the optimum value for MW power, Fe, EDTA dosage was 400 W, 2 g and 0.4 mM, respectively. The possible pathway for degrading the 4-NP was proposed based on GC/MS and HPLC analysis of the degradation intermediates. The concentration change course of the main bio-refractory by-products, the aminophenol formed in the degradation of 4-NP suggested a more efficient degradation and mineralization in Fe/EDTA/MW system. Finally, BOD{sub 5}/COD{sub Cr} of the solution increased from 0.237 to 0.635 after reaction for 18 min, indicating that the biodegradability of wastewater was greatly improved by Fe/EDTA/MW system and would benefit to further treatment by biochemical methods.

  7. Enhanced degradation of 4-nitrophenol by microwave assisted Fe/EDTA process

    International Nuclear Information System (INIS)

    Liu Bo; Li Song; Zhao Yongjun; Wu Wenfei; Zhang Xuxiang; Gu Xueyuan; Li Ruihua; Yang Shaogui

    2010-01-01

    A microwave assisted zero-valent iron oxidation process was studied in order to investigate the synergetic effects of MW irradiation on Fe/EDTA system (Fe/EDTA/MW) treated 4-nitrophenol (4-NP) from aqueous solution. The results indicated that the thermal effect of microwave improved the removal effect of 4-NP and TOC through raising the temperature of the system, as well as the non-thermal effect generated by the interaction between the microwave and the Fe resulting in an increase in the hydrophobic character of Fe surface. During the degradation of 4-NP in Fe/EDTA/MW system, the optimum value for MW power, Fe, EDTA dosage was 400 W, 2 g and 0.4 mM, respectively. The possible pathway for degrading the 4-NP was proposed based on GC/MS and HPLC analysis of the degradation intermediates. The concentration change course of the main bio-refractory by-products, the aminophenol formed in the degradation of 4-NP suggested a more efficient degradation and mineralization in Fe/EDTA/MW system. Finally, BOD 5 /COD Cr of the solution increased from 0.237 to 0.635 after reaction for 18 min, indicating that the biodegradability of wastewater was greatly improved by Fe/EDTA/MW system and would benefit to further treatment by biochemical methods.

  8. New EDTA determination method based on ion chromatography with suppressed conductimetric detection

    International Nuclear Information System (INIS)

    Gyoergy Patzay; Yahya Ramadan; Csilla Tonko

    2014-01-01

    A novel direct method for the determination of EDTA in alkaline radioactive evaporator residue solution was developed and validated based on ion chromatography with suppressed conductimetric detection and anion exchange columns (A Supp 4, 4 mm × 250 mm and A Supp 5, 4 mm × 150 mm). The yttrium-EDTA complex resulted one single chromatographic peak in the eluent and allowed the correct determination of EDTA in an alkaline, high concentration radioactive waste water. Depending on coexisting substances, suitable eluent is 10.0 mM carbonate buffer/pH 10.6 or 10.75 (t R ,Y-EDTA = 7.01 and 6.4 min, respectively). For 10.0 mM carbonate buffer/pH 10.6 and isocratic flow rate of 1.0 cm 3 /min, a linear calibration curve was obtained from 5 to 40 mg/dm 3 (r > 0.999) EDTA. Good resolution was achieved from commonly coexisting anions (chloride, nitrite, nitrate, sulphate, phosphate, bromide and citrate). The developed simple ion chromatographic method was applied for the assay of EDTA in various radioactive alkaline solutions. (author)

  9. Immunological effects of CaEDTA injection: observations in two lead workers.

    Science.gov (United States)

    Sata, F; Araki, S; Sakai, T; Nakata, A; Yamashita, K; Morita, Y; Tanigawa, T; Miki, A

    1997-12-01

    To evaluate the effects of calcium disodium ethylenediamine tetraacetate (CaEDTA) injection on human immune system in relation to exposure to lead, we administered CaEDTA by intravenous injection for 1 hr three times (three consecutive days) a week to two male lead workers. They had been engaged in recycling lead for 31 and 22 years, aged 61 and 53 years (workers 1 and 2), respectively. Before the treatment of CaEDTA, their blood lead concentrations (PbB) were 81 and 68 micrograms/dl, respectively. The administration of CaEDTA had been carried out to worker 1 for 10 weeks and to worker 2 for 6 weeks. A significant decrease in PbB between before and after three-times CaEDTA injection was found in both workers. Significant increases in IgG, IgA, IgM, CD8+, and CD57+ cells were found in worker 1. A significant increase in IgD was found in worker 2. During the study period, IgG in worker 1 and CD4+ cells in worker 2 were gradually increasing. There was a significant negative correlation between IgG and PbB in worker 1. It is suggested that the immunological function such as antibody formation in lead workers might be improved by CaEDTA injection.

  10. Leach of the weathering crust elution-deposited rare earth ore for low environmental pollution with a combination of (NH4)2SO4 and EDTA.

    Science.gov (United States)

    Tang, Jie; Qiao, Jiyang; Xue, Qiang; Liu, Fei; Chen, Honghan; Zhang, Guochen

    2018-05-01

    High concentration of ammonium sulfate, a typical leaching agent, was often used in the mining process of the weathering crust elution-deposited rare earth ore. After mining, a lot of ammonia nitrogen and labile heavy metal fractions were residual in tailings, which may result in a huge potential risk to the environment. In this study, in order to achieve the maximum extraction of rare earth elements and reduce the labile heavy metal, extraction effect and fraction changes of lanthanum (La) and lead (Pb) in the weathering crust elution-deposited rare earth ore were studied by using a compound agent of (NH 4 ) 2 SO 4 -EDTA. The extraction efficiency of La was more than 90% by using 0.2% (NH 4 ) 2 SO 4 -0.005 M EDTA, which was almost same with that by using 2.0% (NH 4 ) 2 SO 4 solution. In contrast, the extraction efficiency of Pb was 62.3% when use 0.2% (NH 4 ) 2 SO 4 -0.005 M EDTA, which is much higher than that (16.16%) achieved by using 2.0% (NH 4 ) 2 SO 4 solution. The released Pb fractions were mainly acid extractable and reducible fractions, and the content of reducible fraction being leached accounted for 70.45% of the total reducible fraction. Therefore, the use of 0.2% (NH 4 ) 2 SO 4 -0.005 M EDTA can not only reduce the amount of (NH 4 ) 2 SO 4 , but also decrease the labile heavy metal residues in soil, which provides a new way for efficient La extraction with effective preventing and controlling environmental pollution in the process of mining the weathering crust elution-deposited rare earth ore. Copyright © 2018 Elsevier Ltd. All rights reserved.

  11. The Effects of EDTA and H2SO4 on Phyto-extraction of Pb from contaminated Soils by Radish

    Directory of Open Access Journals (Sweden)

    T. Mansouri

    2016-10-01

    Full Text Available Introduction: Soil contamination by heavy metals is one of the most important environmental concerns in many parts of the world. The remediation of soil contaminated with heavy metals is necessary to prevent the entry of these metals into the human food chain. Phyto-extraction is an effective, cheap and environmental friendly method which uses plants for cleaning contaminated soils. The plants are used for phytoremediation should have high potential for heavy metals uptake and produce enormous amount of biomass. A major problem facing phyto-extraction method is the immobility of heavy metals in soils. Chemical phyto-extraction is a method in which different acids and chelating substances are used to enhance the mobility of heavy metals in soil and their uptake by plants. The aims of this study were: (a to determine the potential of radish to extract Pb from contaminated soils and (b to assess the effects of different soil amendment (EDTA and H2SO4 to enhance plant uptake of the heavy metal and (c to study the effects of different levels of soil Pb on radish growth and Pb concentrations of above and below ground parts of this plant. Materials and Methods: Soil samples were air dried and passed through a 2 mm sieve and analysed for some physico-chemical properties and then artificially contaminated with seven levels of lead (0, 200, 400, 600, 800 and 1000 mg/kg using Pb(NO32 salt and then planted radish. During the growth period of radish and after the initiation of root growth, the plants were treated with three levels of sulfuric acid (0, 750 and 1500 mg/kg or three levels of EDTA (0, 10 and 20 mg/kg through irrigation water. At the end of growth period, the above and below ground parts of the plants were harvested, washed, dried and digested using a mixture of HNO3, HCl, and H2O2. The concentrations of Pb, N, P and K in plant extracts were measured. Statistical analysis of data was performed using MSTATC software and comparison of means was

  12. Effect of PDGF-BB combined with EDTA gel on adhesion and proliferation to the root surface.

    Science.gov (United States)

    Belal, Mahmoud Helmy; Watanabe, Hisashi; Ichinose, Shizuko; Ishikawa, Isao

    2012-07-01

    Periodontal regeneration using EDTA or PDGF showed promising results, but the effect of combined application was still unclear. This study aimed to verify the effect of EDTA and/or PDGF application on root adhesion and proliferation of PDL fibroblast cells. Eighty specimens were prepared from forty periodontitis teeth and made five groups: (1) diseased (untreated), (2) SRP (scaling root planing), (3) EDTA (24%), (4) PDGF (25 ng/ml) and (5) Combined application of EDTA and PDGF. Periodontal ligament cells were cultured on the above conditioned dentin plate, and SEM examination was preformed and cells were counted within a representative standard area for both cell morphology and density. All groups including untreated showed significantly increase of adhered cells from baseline to 7 days. Among them, rate of increase was much higher in EDTA, PDGF, and combined groups. ANOVA test indicated that the number of cells in PDGF and combined groups was significantly higher than diseased group at 1 day. On day 7, PDGF and combined groups showed significantly higher number of adhesion cells than that found in the diseased, SRP and EDTA groups. Thus, root conditioning with EDTA enhanced cell adhesion more than SRP alone. There was no significant difference of cell number between PDGF and combined group. Combined application of EDTA and PDGF increased significantly PDL cell adhesion than EDTA alone. PDGF alone, however, also showed comparable effect to combined application at all periods. Thus, synergistic effect between PDGF and EDTA was not observed.

  13. Effectiveness of EDTA and EDTA-T brushingm on the removal of root surface smear layer Efeito do EDTA e EDTA-T aplicado de forma ativa na remoção de "smear layer" de superfícies radiculares

    Directory of Open Access Journals (Sweden)

    José Eduardo Cezar Sampaio

    2003-12-01

    Full Text Available The purpose of this study was to compare the removal of root surface smear layer following active application of EDTA gel and EDTA-T (texapon gel in different concentrations (5%, 10%, 15%, 20% and 24%, using scanning electron microscopy. A total of 220 dentin blocks obtained from the root surfaces of extracted teeth were divided into 3 groups: Group I - (control application of saline solution (n = 20; Group II - EDTA gel (pH 7.0 was applied in the following concentrations: 5%, 10%, 15%, 20% and 24% (n = 100; Group III - EDTA-T gel (pH 7.0 applied in the same concentrations described above (n = 100. The photomicrographs were evaluated by one calibrated examiner using a smear layer removal index and following statistical analysis (Kruskal-Wallis test. The results demonstrated that the specimens treated with EDTA and EDTA-T gel presented a better smear layer removal than the control group (p 0.05. Within the limits of this study, it can be concluded that all treatment modalities effectively removed the smear layer from the root surface. The addition of texapon into the EDTA gel formulation did not increase its effectiveness.O objetivo do presente estudo foi comparar, através de microscopia eletrônica de varredura, o efeito da aplicação de gel de EDTA e de EDTA-T (texapon em diferentes concentrações (5%, 10%, 15%, 20% e 24% na remoção de "smear layer" de superfícies radiculares previamente raspadas com instrumentos manuais. Duzentos e vinte espécimes de superfícies radiculares submetidas à raspagem foram divididos em 3 grupos. Grupo I - (controle solução salina (n = 20; Grupo II - gel de EDTA (pH 7,0 nas concentrações de 5%, 10%, 15%, 20% e 24% (n = 100; Grupo III - gel de EDTA-T (pH 7,0 nas concentrações acima descritas (n = 100. As fotomicrografias obtidas foram avaliadas por um examinador calibrado através da aplicação de um índice de remoção de "smear layer", e os dados foram analisados através de análise estat

  14. Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae

    Directory of Open Access Journals (Sweden)

    Lilian Terezinha de Queiroz Leite

    1996-10-01

    Full Text Available Monoclonal antibodies (MABs ivere produced against an etbylenediaminetetraacetate (EDTA extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgGl and IgG2b. The EDTA extract was detected as several bands by silver staining in SDS-PAGE. In the Western blot the bands around 20 KDa reacted with a monoclonal antibody, 47B4D6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohydrate nature, lmmunocytochemistry, using colloidal gold labeling, showed that an EDTA extract determinant recognized by monoclonal antibody 47B4D6, is localized under the outer envelope of serovar icterohaemorrhagiae. Hoe AIAB raised against the EDTA extract was not able to protect hamsters from lethal challenge with virulent homologous leptospires.Anticorpos monoclonais (AcM foram produzidos contra o extrato EDTA obtido de Leptospira interrogans, sorovar icterohaemorrhagiae. Pelo teste de precipitação foram caracterizados como IgM e IgG (IgGl e IgG2. A eletroforese em gel de poliacrilamida do extrato EDTA revelou diversas bandas quando corada pela prata. No "Western blot", as bandas em torno de 20 kDa reagiram com o AcM 47B4D6, foram oxidadas pelo periodato e não digeridas pela pronase, sugerindo que o determinante é de natureza carboidrato. O determinante reconhecido pelo AcM 47B4D6 estã localizado sob o envelope externo como revelado pela imunocitoquímica usando marcação com ouro coloidal. O AcM contra extrato EDTA do sorovar icterohaemorrahagiae não protegeu hamsters quando inoculados com lepstopira homóloga virulenta.

  15. PMR spectroscopy of Ca(II) complexes of ethylenediamine-N,N,N',N' - tetraacetic acid

    International Nuclear Information System (INIS)

    Novomesky, P.; Balgavy, P.; Majer, J.

    1977-01-01

    Proton magnetic resonance spectra for the EDTA complexes of Ca(II) were measured in aqueous solutions as a function of solution pH. It follows from the analysis of chemical shift changes that the nitrogen atoms rather than the carboxylate groups are protonated (as the pH is lowered) in the tetraanion of EDTA. The same conclusion follows for the protonization of the [CaEDTA] 2- complex in the acid pH range where the hydrogen complex [CaH(EDTA)] - is formed. The formation of [Ca(OH)EDTA] 3- was not observed at pH less than 12.6, at [CaEDTA] 2- concentration of 0.5 mol/l. In solutions with a metal-to-ligand molar ratio 1:1.5 two sets of resonance signals appear in the pH range from 12.8 to 6.0, the former for the free ligand and the latter for the stable [CaEDTA] 2- complex. However, from pH 6.0 to 4.35 only one set of broadened signals was observed, which indicates that an exchange process between free ligand, normal [CaEDTA] 2- complex and [CaH(EDTA)] - takes place. (author)

  16. The impact of EDTA on lead distribution and speciation in the accumulator Sedum alfredii by synchrotron X-ray investigation

    International Nuclear Information System (INIS)

    Tian Shengke; Lu Lingli; Yang Xiaoe; Huang Huagang; Brown, Patrick; Labavitch, John; Liao Haibing; He Zhenli

    2011-01-01

    The in vivo localization and speciation of lead (Pb) in tissues of the accumulator Sedum alfredii grown in EDTA-Pb and Pb(NO 3 ) 2 was studied by synchrotron X-ray investigation. The presence of EDTA-Pb in solution resulted in a significant reduction of Pb accumulation in S. alfredii. Lead was preferentially localized in the vascular bundles regardless of treatments but the intensities of Pb were lower in the plants treated with EDTA. Lead was predominantly presented as a Pb-cell wall complex in the plants regardless of its supply form. However, a relatively high proportion of Pb was observed as Pb-EDTA complex when the plant was treated with EDTA-Pb, but as a mixture of Pb 3 (PO 4 ) 2 , Pb-malic, and Pb-GSH when cultured with ionic Pb. These results suggest that EDTA does not increase the internal mobility of Pb, although the soluble Pb-EDTA complex could be transported and accumulated within the plants of S. alfredii. - Research highlights: → The plants of S. alfredii are less effective in taking up Pb from the EDTA-Pb medium. → Pb is mainly localized in the vascular bundles and probably as Pb-cell wall complex. → EDTA does not increase the internal mobility of Pb within the plants of S. alfredii. → The Pb-EDTA complex could be transported and accumulated within the plants. - The plants of S. alfredii are able to transport and accumulate the Pb-EDTA complex, but are less effective in taking up Pb from the EDTA-Pb medium.

  17. The impact of EDTA on lead distribution and speciation in the accumulator Sedum alfredii by synchrotron X-ray investigation

    Energy Technology Data Exchange (ETDEWEB)

    Tian Shengke [MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310029 (China); Lu Lingli, E-mail: linglilulu@gmail.com [MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310029 (China); Yang Xiaoe, E-mail: xyang571@yahoo.com [MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310029 (China); Huang Huagang [MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310029 (China); Brown, Patrick; Labavitch, John [Department of Plant Sciences, University of California, Davis, CA 95616 (United States); Liao Haibing [MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310029 (China); He Zhenli [University of Florida, Institute of Food and Agricultural Sciences, Indian River Research and Education Center, Fort Pierce, FL 34945 (United States)

    2011-03-15

    The in vivo localization and speciation of lead (Pb) in tissues of the accumulator Sedum alfredii grown in EDTA-Pb and Pb(NO{sub 3}){sub 2} was studied by synchrotron X-ray investigation. The presence of EDTA-Pb in solution resulted in a significant reduction of Pb accumulation in S. alfredii. Lead was preferentially localized in the vascular bundles regardless of treatments but the intensities of Pb were lower in the plants treated with EDTA. Lead was predominantly presented as a Pb-cell wall complex in the plants regardless of its supply form. However, a relatively high proportion of Pb was observed as Pb-EDTA complex when the plant was treated with EDTA-Pb, but as a mixture of Pb{sub 3}(PO{sub 4}){sub 2}, Pb-malic, and Pb-GSH when cultured with ionic Pb. These results suggest that EDTA does not increase the internal mobility of Pb, although the soluble Pb-EDTA complex could be transported and accumulated within the plants of S. alfredii. - Research highlights: > The plants of S. alfredii are less effective in taking up Pb from the EDTA-Pb medium. > Pb is mainly localized in the vascular bundles and probably as Pb-cell wall complex. > EDTA does not increase the internal mobility of Pb within the plants of S. alfredii. > The Pb-EDTA complex could be transported and accumulated within the plants. - The plants of S. alfredii are able to transport and accumulate the Pb-EDTA complex, but are less effective in taking up Pb from the EDTA-Pb medium.

  18. Chlorhexidine controlled-release profile after EDTA root surface etching: an in vivo study.

    Science.gov (United States)

    Gamal, Ahmed Y; Kumper, Radi M; Sadek, Hesham S; El Destawy, Mahmoud T

    2011-05-01

    The main objective of the present study was to quantify chlorhexidine (CHX) release after the use of CHX-EDTA root surface treatment as a local-delivery antimicrobial vehicle. Twenty non-smoking patients clinically diagnosed as having moderate-to-severe chronic periodontitis were selected to participate in this study. After cause-related therapy, one site in every patient received defect overfill with CHX gel 2% (20 sites). In addition, twenty contralateral sites received defect fill of CHX gel after 3 minutes of 24% EDTA gel root surface etching (20 sites). Gingival crevicular fluid samples were collected at 1, 3, 7, and 14 days post-therapy. The CHX-EDTA group showed statistically significantly higher levels of CHX than those of the control group at 1, 3, and 7 days. At 14 days, the CHX-EDTA group showed 0.8 mg/mL values. The use of CHX-EDTA root surface treatment as a local-delivery antimicrobial improves CHX substantivity.

  19. Intestinal permeability to [51Cr]EDTA in children with Crohn's disease and celiac disease

    International Nuclear Information System (INIS)

    Turck, D.; Ythier, H.; Maquet, E.; Deveaux, M.; Marchandise, X.; Farriaux, J.P.; Fontaine, G.

    1987-01-01

    [ 51 Cr]EDTA was used as a probe molecule to assess intestinal permeability in 7 healthy control adults, 11 control children, 17 children with Crohn's disease, and 6 children with untreated celiac disease. After subjects fasted overnight, 75 kBq/kg (= 2 microCi/kg) 51 Cr-labeled EDTA was given by mouth; 24-h urinary excretion of [ 51 Cr]EDTA was measured and expressed as a percentage of the total oral dose. Mean and SD were as follows: control adults 1.47 +/- 0.62, control children 1.59 +/- 0.55, and patients with Crohn's disease or celiac disease 5.35 +/- 1.94. The difference between control children and patients was statistically significant (p less than 0.001). These results show that intestinal permeability to [ 51 Cr]EDTA is increased among children with active or inactive Crohn's disease affecting small bowel only or small bowel and colon, and with untreated celiac disease. The [ 51 Cr]EDTA permeability test could facilitate the decision to perform more extensive investigations in children suspected of small bowel disease who have atypical or poor clinical and biological symptomatology

  20. Efficacy of the chelating agent CaEDTA in reversing lead-induced changes in behavior.

    Science.gov (United States)

    Cory-Slechta, D A; Weiss, B

    1989-01-01

    The chelating agent CaEDTA has been reported to reverse the deficits in intellectual function and performance associated with Pb (lead) exposure in children. However, such studies have not included rigorous controls for the intervention procedures per se. The experiments reported here examined reversibility of performance changes in a rat model based on behavior sensitive to low-level Pb exposure. Rats were exposed to 50 ppm sodium or Pb acetate in drinking water from weaning. Performance maintained under a Fixed-Interval schedule of food reinforcement began at 55 days of age. Following the onset of the characteristic increase in short interresponse times (IRTs) associated with low-level Pb exposure after 35 experimental sessions, Pb treatment was terminated. Animals within both the control and Pb groups were then matched on the basis of performance indices and injected daily for 5 days with either saline, 75 mg/kg or 150 mg/kg CaEDTA. Subsequent changes in F1 performance were monitored for 35-60 sessions. No consistent effects of CaEDTA were detected in control animals. CaEDTA treatment failed to reverse the behavioral effects in Pb-exposed animals. If anything, it tended to further increase the proportion of short IRTs. These data suggest that better controlled clinical studies are warranted to evaluate the efficacy of CaEDTA in reversing Pb-induced behavioral effects before its application for these purposes becomes widespread.

  1. A scanning electron microscopy study of root surface smear layer removal after topical application of EDTA plus a detergent

    OpenAIRE

    Sampaio, José Eduardo Cezar; Campos, Flávia Pavan; Pilatti, Gibson Luiz; Theodoro, Letícia Helena; Leite, Fábio Renato Manzolli

    2005-01-01

    The aim of the present study was to compare root surface smear layer removal following topical application of EDTA and EDTA-T (Texapon). Extracted human teeth had their cementum removed and were mechanically scaled. A total of 220 root specimens were obtained and were randomly assigned to the following groups: I-saline solution(control), II-EDTA; III-EDTAT. Groups II and III specimens were assigned to different EDTA gel concentrations: 5%, 10%, 15%, 20% and 24%. Smear layer removal score was ...

  2. Synthesis of LaNiO3 perovskite type by chelating precursor method using EDTA: optimization of chelating content

    International Nuclear Information System (INIS)

    Santos, Jose Carlos dos; Pedrosa, Anne Michelle Garrido; Mesquita, Maria Eliane; Souza, Marcelo Jose Barros de

    2011-01-01

    The perovskites are strategic materials due their catalytic, electronic and magnetic properties. These properties are influenced by the calcination and synthesis conditions. In this work was carried out the synthesis of LaNiO 3 perovskite type by chelating precursor method using EDTA and also was studied the optimization of the EDTA content in the synthesis. The synthesized materials were characterized by X-ray diffraction (XRD), thermal gravimetric analysis (TG) and Infrared Spectroscopy (FTIR). In the optimization of the EDTA content the lowest ratio of metal / EDTA used was 1.0 / 0.1, where it was possible to obtain monophasic perovskite. (author)

  3. A comparative study on EDTA and coronaliy advanced flap technique in the treatment of human gingival recessions

    Directory of Open Access Journals (Sweden)

    Khoshkhoo Nejad AA

    2003-08-01

    Full Text Available Statement of Problem: Treatment of gingival recession defect and covering denuded root surfaces is one of the goals in periodontal therapy and several surgical techniques have been suggested in this field."nPurpose: The aim of this study was to perform a comparison on coronaliy repositioned flap procedure with and without the use of ethylenediaminoteraacetic acid (EDTA. 24%, pH=7 in the treatment of"nrecession defects."nMaterial and Methods: In this randomized clinical trial study, 16 patients, aged 17-60 years, with a total of 27 miller class 1 isolated buccal gingival recession type defects of at least 2mm depth, and based"non special criteria were investigated. After initial therapy, surgical recession coverage was performed as coronaliy advanced flap technique and EDTA gel conditioning (test or coronaliy advanced flap alone"n(control. Clinical examination including assessments of oral hygiene, recession depth (RD, recession width (RW, width of keratinized tissue (KT, probing depth (PD and probing attachment level (PAL"nwere performed before and 1, 2, 3 months after surgical treatment."nResults: The mean of initial RD, RW, KT, PT and PAL in the test group was 2.73, 3.17, 3.13, 1.1 and 3.83mm respectively and in the control group was 2.56, 3.03, 3.67, 1.25, 3.92mm respectively. The mean of these parameters 3 months after treatment in the test group were changed to 0.46, 1.97, 2.65, 0.67, 1.1 mm, corresponding figures for control teeth were 0.85, 2.98, 2.75, 1, 1.94, respectively. At 3 months after treatment the mean root coverage amounted to 83% (test and 67% (control which was a statistically significant difference (P=0.0067. Although a significant clinical difference was observed regarinding root coverage level, all other clinical variables were not statistically different, with the exception of probing attachment level (P=0.005."nConclusion: It was suggested that EDTA gel (24%, PLT=7 for 3 minutes as root conditioner and the coronaliy

  4. Role of Various Extractants in Removing Group-IIB Elements of Soils Incubated with EDTA

    Directory of Open Access Journals (Sweden)

    Tanmoy Karak

    2004-01-01

    Full Text Available This paper presents the results of an experimental investigation undertaken to evaluate different extractant solutions viz. HCl, Mg(NO32, and DTPA with the range of concentration from 0.001 to 0.1N after incubation with group-IIB metals (Zn, Cd, and Hg and EDTA to understand the capability to remove Zn, Cd, and Hg from soils. Two noncontaminated soils, one acidic (GHL and the other alkaline (KAP, in reaction were taken from an agricultural field of West Bengal, India for this investigation. Experiments were conducted on these two soils spiked with ZnII, CdII, and HgII in concentrations of 612, 321, and 215 mg/kg for soil GHL and 778, 298, and 157 mg/kg for soil KAP, respectively, which simulate typical electroplating waste contamination. The removal of Zn, Cd, and Hg in soil GHL within the range of HCl concentrations was 8.2–16.5, 12.2–19.1, and 4.3–6.9 whereas these were 6.5–7.6, 8.5–14.1, and 3.2–5.2 in soil KAP. The removal of Zn, Cd, and Hg in soil GHL within the range of Mg(NO32 concentrations were 12.2–28.5, 19.1–24.6, and 18.2–19.1 whereas these were 9.1–12.1, 8.3–12.1, and 10.6–48.1 in soil KAP. For DTPA extractant, the percent removal of metal was found to be significantly higher than the other two extractants, which corroborates that DTPA is a better extractant for soil cleaning.

  5. Comparison between total and renal plasma clearance of [51Cr]EDTA

    International Nuclear Information System (INIS)

    Broechner-Mortensen, J.; Roedbro, P.

    1976-01-01

    The total plasma (E 1 ) and renal plasma (E 2 ) clearances of [ 51 Cr]EDTA were measured simultaneously in 40 patients with a wide range of renal function. The regression equation was E 1 = 0.997E 2 + 4.1; the slope of the regression line was not significantly different from 1.00, and the intercept differed significantly from zero. The results suggest that [ 51 Cr]EDTA has a significant extrarenal clearance of approximately 4 ml/min, independent of renal function. The data are used to correct the total [ 51 Cr]EDTA plasma clearance, measured by a simplified method, to yield a rapid, accurate and precise measurement of glomerular filtration rate for routine clinical use. (Auth.)

  6. 'Scan GFR' as an alternative to 51Cr-EDTA clearance

    International Nuclear Information System (INIS)

    Osman, E.A.; Clarke, M.B.; Barrett, J.J.; Parsons, V.; Dulwich Hospital, London

    1989-01-01

    In this report 39 patients had their GFR assessed by 51 Cr-EDTA in a single shot-multiple sampling technique and also by measuring the quantitative renal uptake of 99m Tc-DTPA during a standard renal imaging test. The results showed a correlation coefficient of 0.96 between the two techniques. By using linear regression analysis the GFR was derived from the % renal uptake of DTPA in a further series of 24 patients in whom the 51 Cr-EDTA method was also employed. The two methods were compared and showed a correlation coefficient of 0.94 with a mean GFR difference of 7 ± 4.5 ml/min over a 51 Cr-EDTA range of 38-150 ml/min. (orig.) [de

  7. Interactions between 59Fe(14C)EDTA and soils containing calcium carbonate

    International Nuclear Information System (INIS)

    Hargitai-Toth, A.; Konya, J.

    1991-01-01

    Interaction between FeEDTA and calcareous soils was followed over a period of four weeks using a radiotracer technique, and a kinetic evaluation of the results was performed. 59 Fe served to determine the quantity of iron, 14 C to assay for EDTA and 45 Ca to measure calcium. During the experiment, i.e. within four weeks in case of the chernozem soil 61% and in case of the clayey meadow soil 51% of the iron chelate disappeared from the solution. The loss in soluble iron was partly due to a rapid sorption process of about an hour and partly due to the slow decomposition of FeEDTA to Fe(OH) 3 . The two processes could be separated using the Christiansen equation. (author) 9 refs.; 1 figs.; 2 tabs

  8. [51Cr]EDTA intestinal permeability in children with cow's milk intolerance

    International Nuclear Information System (INIS)

    Schrander, J.J.; Unsalan-Hooyen, R.W.; Forget, P.P.; Jansen, J.

    1990-01-01

    Making use of [ 51 Cr]EDTA as a permeability marker, we measured intestinal permeability in a group of 20 children with proven cow's milk intolerance (CMI), a group of 17 children with similar complaints where CMI was excluded (sick controls), and a group of 12 control children. [ 51 Cr]EDTA test results (mean +/- SD) were 6.85 +/- 3.64%, 3.42 +/- 0.94%, and 2.61 +/- 0.67% in the group with CMI, the sick control, and the control group, respectively. When compared to both control groups, patients with cow's milk intolerance (CMI) showed a significantly increased small bowel permeability. We conclude that the [ 51 Cr]EDTA test can be helpful for the diagnosis of cow's milk intolerance

  9. (51Cr)EDTA intestinal permeability in children with cow's milk intolerance

    Energy Technology Data Exchange (ETDEWEB)

    Schrander, J.J.; Unsalan-Hooyen, R.W.; Forget, P.P.; Jansen, J. (Academic Hospital Maastricht (Netherlands))

    1990-02-01

    Making use of ({sup 51}Cr)EDTA as a permeability marker, we measured intestinal permeability in a group of 20 children with proven cow's milk intolerance (CMI), a group of 17 children with similar complaints where CMI was excluded (sick controls), and a group of 12 control children. ({sup 51}Cr)EDTA test results (mean +/- SD) were 6.85 +/- 3.64%, 3.42 +/- 0.94%, and 2.61 +/- 0.67% in the group with CMI, the sick control, and the control group, respectively. When compared to both control groups, patients with cow's milk intolerance (CMI) showed a significantly increased small bowel permeability. We conclude that the ({sup 51}Cr)EDTA test can be helpful for the diagnosis of cow's milk intolerance.

  10. Temporal Lob Epilepsi'sinde L-Arginine ve CaEDTA'nın Etkileri

    OpenAIRE

    NOYAN, Behzat

    2005-01-01

    Bu çalışmada, bir nitrik oksit (NO-) prekürsörü olan L-Arginine ve bir ekstrasellüler çinko şelatörü olan CaEDTA'nın pilokarpine HCl ile oluşturulan kısa süreli epileptik nöbet üzerine etkileri araştırıldı. Deney, nöbet kontrol (serum fizyolojik 10 µl, i.c.v., ve sonra 380 mg/kg pilokarpine HCl i.p.), L-Arginine (150 µg/10 µl, i.c.v.), CaEDTA (100 mM, 10 µl, i.c.v.), L-Arginine+CaEDTA olmak üzere 4 gruptan oluştu. Enjeksiyonlardan sonra iki saat boyunca nöbet davranışları gözlemlenen ...

  11. A laboratory and field evaluation of the mobility of cobalt-60/EDTA

    International Nuclear Information System (INIS)

    Jones, T.L.; Gee, G.W.; Kirkham, R.R.; Swanson, J.L.

    1983-01-01

    We have observed a time and soil type dependence in the ability of the organic complexant EDTA to keep cobalt-60 in solution. Test results indicate that short-term adsorption tests lasting 5 days or less can be misleading. In short-term tests using cobalt-60/EDTA and soil from the Hanford site, low sorption in batch tests and high mobility in column tests were observed. During long-term batch test using cobalt-60/EDTA, the percentage of cobalt remaining in solution decreased from 90% after 7 days to less than 10% after 500 days. In laboratory and field column tests where low water flow rates allowed long contact time, virtually no cobalt movement was observed even though in the field test tritium was transported over 4 meters. Long-term batch tests using cobalt-60/EDTA and soil from Savannah River burial grounds showed that cobalt remainin in solution dropped to 30% of the total cobalt added after 5 days and to less than 1% after 15 days. Batch tests using soil from Oak Ridge burial grounds were less dramatic showing cobalt in solution decreasing from 90% after 5 days to 70% after 35 days. The cobalt-60/EDTA complex appears to be dissociating and leaving uncomplexed cobalt which is readily sorbed. The dissociation seems to be rather complete in Hanford and Savannah River soil but limited in the Oak Ridge soil. The implication to waste management is that the potential for transport of cobalt by EDTA may not be as serious at all burial sites as once thought

  12. Influence of EDTA2− on the hydrothermal synthesis of CdTe nanocrystallites

    International Nuclear Information System (INIS)

    Gong Haibo; Hao Xiaopeng; Wu Yongzhong; Cao Bingqiang; Xu Hongyan; Xu Xiangang

    2011-01-01

    Transformation from Te nanorods to CdTe nanoparticles was achieved with the assistance of EDTA as a ligand under hydrothermal conditions. Experimental results showed that at the beginning of reaction Te nucleated and grew into nanorods. With the proceeding of reaction, CdTe nucleus began to emerge on the surface, especially on the tips of Te nanorods. Finally, nearly monodispersed hexagonal CdTe nanoparticles with diameters of about 200 nm were obtained. The effects of EDTA on the morphology and formation of CdTe nanoparticles were discussed in consideration of the strong ligand-effect of EDTA, which greatly decreased the concentration of Cd 2+ . Furthermore, the possible formation process of CdTe nanoparticles from Te nanorods was further proposed. The crystal structure and morphology of the products were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM). - Graphical Abstract: Firstly, Te nucleated and grew into nanorods in the presence of EDTA 2− . Then CdTe nucleus began to emerge on Te nanorods and finally monodispersed CdTe nanoparticles were obtained. Highlights: ► EDTA serves as a strong ligand with Cd 2+ . ► The existence of EDTA constrains the nucleation of CdTe and promotes the formation of Te nanorods. ► With the proceeding of reaction, CdTe nucleus began to emerge on the surface, especially on the tips of Te nanorods. ► Nearly monodispersed hexagonal CdTe nanoparticles with diameters of about 200 nm were finally obtained.

  13. The use of EDTA and DTPA for accelerating the removal of deposited transuranic elements from humans

    CERN Document Server

    Spoor, N L

    1977-01-01

    EDTA and DTPA have been prominent among the chelating agents used to increase the rate of excretion of certain deposited heavy metals from the human body. Since 1959, DTPA, administered either by intravenous injection or by aerosol inhalation, has been widely used to treat workers contaminated by plutonium or a higher actinide. In this report, an attempt is made to assess the toxicities of EDTA and DTPA and to evaluate the effectiveness and safety of DTPA as a drug for removing deposited transuranic elements.

  14. Evaluation of calcium di-sodium EDTA intramuscular injection using gamma scintigraphy against heavy metal poisoning

    International Nuclear Information System (INIS)

    Kumar, N.; Soni, S.; Mehra, L.; Mittal, G.; Nishad, D.K.; Bhatnagar, A.; Singh, T.; Ahmad, F.J.

    2010-01-01

    Full text: Heavy metals become toxic when they are not metabolized by the body and accumulate in soft tissues. These metals may enter the body through food, water, air or absorption through skin when an individual is exposed to areas with high heavy metal content such as agriculture, pharmaceutical manufacturing, paint and heavy metal industries or residential settings. Majority of population commonly get affected by heavy metal poisoning. Heavy metal poisoning is more likely to result through inhalation or ingestion route. Aim of the present study is to provide an easy, effective and controlled chelating therapy against heavy metal using Ca-disodium EDTA intramuscular injection. Ca-disodium-EDTA and Sesame oil were procured from Merck, India and Sigma Aldrich, USA respectively. Ca-Na 2 EDTA radiolabeling with 99m Tc-pertechnetate was standardized using stannous ions as reducing agent. The hypothesis of present work is to maintain effective concentration of chelating agent in blood for prolong time period. To test this hypothesis, we studied 1% Ca-Na 2 EDTA blood bioavailability after intramuscular depot administration in new zealand white rabbit. The optimized radiolabeled intramuscular formulation was evaluated for its dissolution and permeation studies in isotonic buffer solution were envisaged through radiometry. Sub-acute toxicity studies were performed after a single dose intramuscular administration of 1% Ca-Na 2 EDTA in Sprague Dawley rats. Radiolabeled Ca-disodium-EDTA (>95% labeled) was found to be fairly stable up to 24 h in physiological solution (Serum) as well as in normal saline with negligible degradation of 2.5% and 5.0% respectively, thereby indicating high stability of radiolabeled product. In-vitro data indicates that control release pattern (Cmax 24 hrs) with effective concentration up to seven days. 99m Tc-CaNa 2 EDTA retention at depot site was estimated up to 3 days by scintigraphy. Subacute toxicity studies done by histopathological

  15. The effect of EDTA in attachment gain and root coverage.

    Science.gov (United States)

    Kassab, Moawia M; Cohen, Robert E; Andreana, Sebastiano; Dentino, Andrew R

    2006-06-01

    Root surface biomodification using low pH agents such as citric acid and tetracycline has been proposed to enhance root coverage following connective tissue grafting. The authors hypothesized that root conditioning with neutral pH edetic acid would improve vertical recession depth, root surface coverage, pocket depth, and clinical attachment levels. Twenty teeth in 10 patients with Miller class I and II recession were treated with connective tissue grafting. The experimental sites received 24% edetic acid in sterile distilled water applied to the root surface for 2 minutes before grafting. Controls were pretreated with only sterile distilled water. Measurements were evaluated before surgery and 6 months after surgery. Analysis of variance was used to determine differences between experimental and control groups. We found significant postoperative improvements in vertical recession depth, root surface coverage, and clinical attachment levels in test and control groups, compared to postoperative data. Pocket depth differences were not significant (P<.01).

  16. Glomerular filtration rate by {sup 51}chomium and {sup 113m}indium labeled EDTA in horses; Taxa de filtracao glomerular pelo EDTA marcado com {sup 51}cromo e com {sup 113m}indio em equinos

    Energy Technology Data Exchange (ETDEWEB)

    Maliska, C.; D' Almeida, J.; Pellegrini, P.M.; Schimit, T.S. [Hospital Central do Exercito (HCE), Rio de Janeiro, RJ (Brazil). Servico de Medicina Nuclear; Pinho, W.R. [Centro de Ensino Superior, Valenca, RJ (Brazil). Fac. de Medicina Veterinaria; Lima, J.E.T. [Instituto Nacional do Cancer (INCa), Rio de Janeiro, RJ (Brazil). Servico de Medicina Nuclear

    2009-07-01

    The glomerular filtration rate was determined in nine healthy horses, six male and three female, aged two to 12-year-old, by means of {sup 51}Cr and {sup 113m}In labeled EDTA single injection technique. The glomerular filtration rate was calculated from the plasma disappearance curve and the volume of distribution of the radiotracer, {sup 51}Cr-EDTA or {sup 113m}In-EDTA. The result (mean +- standard deviation) was 148.80 +- 26.42 m L.min{sup -1}.100 kg. It is concluded that the measurement of glomerular filtration rate by {sup 51}Cr-EDTA or {sup 113m}In-EDTA by single injection technique eliminates the bladder catheterization, and for its simplicity, convenience, accuracy, and low dose of radiation, can be used in horses as a method of choice in clinical routine. (author)

  17. Microbial reduction of [Co(III)-EDTA]⁻ by Bacillus licheniformis SPB-2 strain isolated from a solar salt pan.

    Science.gov (United States)

    Paraneeiswaran, Arunachalam; Shukla, Sudhir K; Prashanth, K; Rao, T Subba

    2015-01-01

    Naturally stressed habitats are known to be repositories for novel microorganisms with potential bioremediation applications. In this study, we isolated a [Co(III)-EDTA](-) reducing bacterium Bacillus licheniformis SPB-2 from a solar salt pan that is exposed to constant cycles of hydration and desiccation in nature. [Co(III)-EDTA](-) generated during nuclear waste management process is difficult to remove from the waste due to its high stability and solubility. It is reduced form i.e. [Co(II)-EDTA](2-) is less stable though it is toxic. This study showed that B. licheniformis SPB-2 reduced 1mM [Co(III)-EDTA](-) in 14 days when grown in a batch mode. However, subsequent cycles showed an increase in the reduction activity, which was observed up to four cycles. Interestingly, the present study also showed that [Co(III)-EDTA](-) acted as an inducer for B. licheniformis SPB-2 spore germination. Vegetative cells germinated from the spores were found to be involved in [Co(III)-EDTA](-) reduction. More detailed investigations showed that after [Co(III)-EDTA](-) reduction, i.e. [Co(II)-EDTA](2-) complex was removed by B. licheniformis SPB-2 from the bulk liquid by adsorption phenomenon. The bacterium showed a D10 value (radiation dose required to kill 90% cells) of ∼250 Gray (Gy), which signifies the potential use of B. licheniformis SPB-2 for bioremediation of moderately active nuclear waste. Copyright © 2014 Elsevier B.V. All rights reserved.

  18. The effect of 17% EDTA and MTAD on smear layer removal and on erosion of root canal dentin when used as final rinse: An in vitro SEM study

    Directory of Open Access Journals (Sweden)

    Vishal A Mahajan

    2010-01-01

    Full Text Available Background: The aim of the present study was to evaluate and compare the ability of a mixture of tetracycline isomer, citric acid and detergent (MTAD and ethylene diamine tetra-acetic acid (EDTA in removing the smear layer along with their effects on peritubular and intertubular dentinal structures by scanning electron microscopic (SEM examination. Materials and Methods: Thirty recently extracted maxillary and mandibular single-rooted human teeth were divided into 3 groups and prepared to an apical size of 30. In Group 1, 2, and 3, MTAD, EDTA, and distilled water were used, respectively, as a final rinse solution to remove the smear layer. The specimens were subjected to SEM evaluation for the presence or absence of the smear layer and degree of erosion using a scoring system. Results: The result showed that MTAD shows better smear layer removing ability and does not significantly change the structure of dentinal tubules. Conclusion: MTAD is an efficient solution for the removal of the smear layer, especially in the apical third of root canals, and does not significantly change the structure of the dentinal tubules.

  19. Glomerular filtration rate measured by {sup 51}Cr-EDTA clearance: evaluation of captopril-induced changes in hypertensive patients with and without renal artery stenosis

    Energy Technology Data Exchange (ETDEWEB)

    Chaves, Anna Alice Rolim; Buchpiguel, Carlos Alberto; Praxedes, Jose Nery; Bortolotto, Luiz Aparecido; Sapienza, Marcelo Tatit, E-mail: annaalice100@yahoo.com.b [Universidade de Sao Paulo (USP), SP (Brazil). Faculdade de Medicina. Dept. de Neurologia

    2010-07-01

    Introduction: renal artery stenosis can lead to renovascular hypertension; however, the detection of stenosis alone does not guarantee the presence of renovascular hypertension. Renovascular hypertension depends on activation of the renin-angiotensin system, which can be detected by functional tests such as captopril renal scintigraphy. A method that allows direct measurement of the baseline and post-captopril glomerular filtration rate using chromium-51 labeled ethylenediamine tetraacetic acid ({sup 51}Cr-EDTA) could add valuable information to the investigation of hypertensive patients with renal artery stenosis. The purposes of this study were to create a protocol to measure the baseline and post-captopril glomerular filtration rate using {sup 51}Cr-EDTA, and to verify whether changes in the glomerular filtration rate permit differentiation between hypertensive patients with and without renal artery stenosis. Methods: this prospective study included 41 consecutive patients with poorly controlled severe hypertension. All patients had undergone a radiological investigation of renal artery stenosis within the month prior to their inclusion. The patients were divided into two groups: patients with (n=21) and without renal artery stenosis, (n=20). In vitro glomerular filtration rate analysis ({sup 51}Cr-EDTA) and {sup 99m}Tc-DMSA scintigraphy were performed before and after captopril administration in all patients. Results: the mean baseline glomerular filtration rate was 48.6+-21.8 ml/kg/1.73 m{sup 2} in the group with renal artery stenosis, which was significantly lower than the GFR of 65.1+-28.7 ml/kg/1.73m{sup 2} in the group without renal artery stenosis (p=0.04). Captopril induced a significant reduction of the glomerular filtration rate in the group with renal artery stenosis (to 32.6+-14.8 ml/kg/1.73m{sup 2}, p=0.001) and an insignificant change in the group without RAS (to 62.2+-23.6 ml/kg/1.73m{sup 2}, p=0.68). Scintigraphy with technetium-99m dimercapto

  20. Glomerular filtration rate measured by 51Cr-EDTA clearance: evaluation of captopril-induced changes in hypertensive patients with and without renal artery stenosis

    International Nuclear Information System (INIS)

    Chaves, Anna Alice Rolim; Buchpiguel, Carlos Alberto; Praxedes, Jose Nery; Bortolotto, Luiz Aparecido; Sapienza, Marcelo Tatit

    2010-01-01

    Introduction: renal artery stenosis can lead to renovascular hypertension; however, the detection of stenosis alone does not guarantee the presence of renovascular hypertension. Renovascular hypertension depends on activation of the renin-angiotensin system, which can be detected by functional tests such as captopril renal scintigraphy. A method that allows direct measurement of the baseline and post-captopril glomerular filtration rate using chromium-51 labeled ethylenediamine tetraacetic acid ( 51 Cr-EDTA) could add valuable information to the investigation of hypertensive patients with renal artery stenosis. The purposes of this study were to create a protocol to measure the baseline and post-captopril glomerular filtration rate using 51 Cr-EDTA, and to verify whether changes in the glomerular filtration rate permit differentiation between hypertensive patients with and without renal artery stenosis. Methods: this prospective study included 41 consecutive patients with poorly controlled severe hypertension. All patients had undergone a radiological investigation of renal artery stenosis within the month prior to their inclusion. The patients were divided into two groups: patients with (n=21) and without renal artery stenosis, (n=20). In vitro glomerular filtration rate analysis ( 51 Cr-EDTA) and 99m Tc-DMSA scintigraphy were performed before and after captopril administration in all patients. Results: the mean baseline glomerular filtration rate was 48.6±21.8 ml/kg/1.73 m 2 in the group with renal artery stenosis, which was significantly lower than the GFR of 65.1±28.7 ml/kg/1.73m 2 in the group without renal artery stenosis (p=0.04). Captopril induced a significant reduction of the glomerular filtration rate in the group with renal artery stenosis (to 32.6±14.8 ml/kg/1.73m 2 , p=0.001) and an insignificant change in the group without RAS (to 62.2±23.6 ml/kg/1.73m 2 , p=0.68). Scintigraphy with technetium-99m dimercapto-succinic acid (DMSA) did not show

  1. Phytic Acid-to-Iron Molar Ratio Rather than Polyphenol Concentration Determines Iron Bioavailability in Whole-Cowpea Meal among Young Women

    NARCIS (Netherlands)

    Abizari, A.R.; Moretti, D.; Zimmerman, M.B.; Armar-Klemesu, M.; Brouwer, I.D.

    2012-01-01

    Limited data exist on iron absorption from NaFeEDTA and FeSO(4) in legume-based flours. The current study compared iron absorption from NaFeEDTA and FeSO(4) as fortificants within and between red and white varieties of cowpea with different concentrations of polyphenols (PP) but similar phytic acid

  2. Metal decomposition rates or 111In-DPTA and EDTA conjugates of monoclonal antibodies in vivo

    International Nuclear Information System (INIS)

    Goodwin, D.A.; McTigue, M.; Chaovapong, W.; Meares, C.F.; McCall, M.J.

    1986-01-01

    We have studied the metal chelate decomposition rates in vivo in both 111 In-labelled benzyl EDTA and DTPA (bicyclic anhydride) conjugates of monoclonal anti-IAsup(k) IgG2a with identical Kα = 1 x 10 11 M -1 in both Ag + ve and Ag -ve mice. Twenty μCi was given i.v. and whole body counting done immediately and daily for 10 days. Half the mice in each group received i.p. injections of 5.0 mg CaNa 2 EDTA chase (Versenate) to facilitate urinary excretion of free 111 In. 50% of control 111 In-citrate remained at nine days but only 8% with chase. No significant loss of 111 In with chase occurred with C 1 substituted EDTA conjugates. A 19% increase in excretion was demonstrated with the chase in mice give DTPA conjugates (1.9% per day). While this will not interfere with radioimmunoimaging up to 24 h after injection, waiting periods of a week or longer will produce significant background of free 111 In in the reticuloendothelial system, RES. 111 In-EDTA stability was important in accurate metabolic rate measurements of anti-IAsup(k); T1/2 = 7.0 days in Ag-ve mice, T1/2 = 9.3 days in Ag-ve mice. (author)

  3. Ionic strength dependence of stability constants, complexation of Molybdenum(V I) with EDTA

    International Nuclear Information System (INIS)

    Zare, K.; Majlesi, K.; Teimoori, F.

    2002-01-01

    The stability constant of Mo (Vi) complexes with EDTA in aqueous solution has been determined by various authors using different techniques, but according to literature, no work has been reported on ionic strength dependence of these complexes. The present work describes the complexation of Mo (Vi) with EDTA in an ionic strength range of 0.1 to 1.0 moldm - 3 s odium perchlorate at 25 d ig C . The complexation of molybdenum (Vi) with EDTA was investigated in aqueous solution ranging in ph from 5 to 7 using UV spectrophotometric techniques. The composition of the complex was determined by the continuous variations method. It was shown that molybdenum (Vi) forms a 2:1 complex with EDTA of the type (MoO 3 ) 2 L - 4 a t ph =5.5 The parameters that define the dependence on ionic strength were analyzed with the aim of obtaining further information regarding to their variation as a function of the charges involved in the complex reaction. Moreover, a Debye-Huckel type equation makes it possible to estimate a stability constant at a fixed ionic strength when its value is known at another ionic media in the range of 0.1 3 . Therefore the evaluation may make a significant contribution solving many analytical and speciation problems

  4. Intraparticle diffusion of rare earths in porous ion exchanger rounding by EDTA solution

    International Nuclear Information System (INIS)

    Ling Daren; Xie Weije

    1991-01-01

    The self-diffusion of rate earth (RE) isotopes in porous cation exchangers with various radii or different pore structures rounding by EDTA solution was studied. The intraparticle effective diffusivity De was calculated by Boyd's method and Kataoka's bi-disperse pore model, and through further calculation the solid phase diffusivity Dg and macropore diffusivity Dp were also obtained. (author)

  5. Super water-absorbing new material from chitosan, EDTA and urea.

    Science.gov (United States)

    Narayanan, Abathodharanan; Dhamodharan, Raghavachari

    2015-12-10

    A new, super water-absorbing, material is synthesized by the reaction between chitosan, EDTA and urea and named as CHEDUR. CHEDUR is probably formed through the crosslinking of chitosan molecules (CH) with the EDTA-urea (EDUR) adduct that is formed during the reaction. CHEDUR as well as the other products formed in control reactions are characterized extensively. CHEDUR exhibits a very high water uptake capacity when compared with chitosan, chitosan-EDTA adduct, as well as a commercial diaper material. A systematic study was done to find the optimum composition as well as reaction conditions for maximum water absorbing capacity. CHEDUR can play a vital role in applications that demand the rapid absorption and slow release of water such as agriculture, as a three in one new material for the slow release of urea, water and other metal ions that can be attached through the EDTA component. The other potential advantage of CHEDUR is that it can be expected to degrade in soil based on its chitosan backbone. The new material with rapid and high water uptake could also find potential applications as biodegradable active ingredient of the diaper material. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. KINETICS OF THE OXIDATION OF FERROUS CHELATES OF EDTA AND HEDTA IN AQUEOUS-SOLUTION

    NARCIS (Netherlands)

    WUBS, HJ; BEENACKERS, AACM

    1993-01-01

    The kinetics of the reaction of oxygen with ferrous chelates of EDTA and HEDTA was studied in a stirred cell reactor under industrial conditions. The temperature was varied from 20 to 60-degrees-C and the concentration of the ferrous chelate ranged from 0 to 100 mol/m3. The initial pH was 7.5. Under

  7. Characteristics of mixed bacterial cultures degarding EDTA, isolated from Czech sources

    Czech Academy of Sciences Publication Activity Database

    Ivanova, V.; Šafaříková, Miroslava; Vassileva, A.; Tonkova, A.

    2004-01-01

    Roč. 51, č. 3 (2004), s. 306-311 ISSN 0477-0250. [ Food Science, Techniques and Technologies 2004. Plovdiv, 27.10.2004-29.10.2004] R&D Projects: GA AV ČR IBS6087204 Institutional research plan: CEZ:AV0Z6087904 Keywords : EDTA * biodegradation Subject RIV: EE - Microbiology, Virology

  8. The separation of heavy lanthanons with NH4-EDTA as the eluent. The ionites comparison

    International Nuclear Information System (INIS)

    Frank, B.; Wolodkiewicz, W.

    1980-01-01

    Using two types of cation exchangers: Zerolit 225 x 8 and Wofatit KPS there was carried out the comparison of the ion exchange separation efficiency of the rare earth elements. The solution of NH 4 -EDTA with increasing pH gradient was used as the eluent. In these conditions no distinct differences in the separation of rare earth elements were found. (author)

  9. Impedance response characteristics of iron oxide interface in the EDTA solutions

    International Nuclear Information System (INIS)

    Sawa, Tosio; Higuchi, Shigeo; Kataoka, Ichiro; Ito, Hisao.

    1986-01-01

    The relationship between the dissolution and the surface conditions of Fe 3 O 4 were studied in the various conditions of EDTA solutions by means of the A · C impedance measurement. From the experimental results obtained, surface layer of Fe 3 O 4 electrode can be expressed with electrical equivalent circuit that have capacitance and reaction resistance in the electrical double layer. In the Na 2 SO 4 solution without occuring dissolution, reaction resistance was estimated as 314 kΩ · cm 2 and capacitance was 203 μF/cm 2 . In the EDTA solutions, reaction resistance decreases along with dissolution of Fe 3 O 4 . The factors to make decrease reaction resistance are EDTA concentration, pH and temperature of the solutions. In contrast with this, the factor to increase it is dissolved oxygen in the solutions. The reciprocal value of reaction resistance agrees well with the rate of dissolution. On the other hand, when the electrode potential was maintained under the cathodic polarization in the EDTA solutions, impedances of electrode surface showed the lower value than that in the immersion condition. And apparent resistance came near to 0 at the potential of -2.0 V in all the range of frequency. Fe 3 O 4 electrodes pretreated with the cathodic polarization exhibited the characteristic impedance response that were caused by the change of electrode surface and the deposites such as iron hydroxide. (author)

  10. A pulse radiolysis study of the formation and reactions of reduced metal EDTA complexes

    International Nuclear Information System (INIS)

    Buitenhuis, R.

    1977-01-01

    The construction of a computerized pulse radiolysis system with available means appropriate for the wavelength interval between 300 and 1000 nm is described. The investigation of the radiolysis of aqueous solutions of EDTA complexes in the presence of alcohols is discussed

  11. Effect of sugarcane vinasse and EDTA on cadmium phytoextraction by two saltbush plants.

    Science.gov (United States)

    Eissa, Mamdouh A

    2016-05-01

    Although the use of saltbush plants in metal phytoremediation is well known, there is little information about the impact of sugarcane vinasse (SCV) and EDTA on metal uptake. Heavily cadmium-polluted soil (38 mg kg(-1) Cd) was used in pot and incubation experiments to investigate the Cd phytoextraction potential of wavy saltbush (Atriplex undulata) and quail saltbush (Atriplex lentiformis). EDTA at rates of 3, 6, and 10 mM kg(-1) soil and SCV at rates of 7, 15, and 30 mL kg(-1) soil were added to the polluted soil. The application of EDTA significantly (P = 0.002) reduced the growth of saltbush plants; on the other hand, SCV improved the growth. Both EDTA and SCV increased the availability and root-to-shoot transfer of Cd. The plants of A. lentiformis grown on the soil amended with the highest rate of SCV were able to remove 20.4 % of the total soil Cd during a period of 9 months. Based on the obtained results, it may be concluded that A. lentiformis and sugarcane vinasse could be more effective in the phytoextraction of Cd from the polluted soils.

  12. Chelate-assisted phytoextraction: effect of EDTA and EDDS on copper uptake by Brassica napus L.

    Directory of Open Access Journals (Sweden)

    TIJANA M. ZEREMSKI-ŠKORIĆ

    2010-09-01

    Full Text Available Chelate-assisted phytoextraction is proposed as an effective approach for the removal of heavy metals from contaminated soil through the use of high biomass plants. The aim of the present study was to compare the efficiency of the two chelators: EDTA and biodegradable EDDS in enhancing Cu uptake and translocation by Brassica napus L. grown on moderately contaminated soil and treated with increasing concentrations of EDTA or EDDS. Increasing amounts of EDDS caused serious growth suppression of B. napus and an increase in shoot metal concentrations. Growth suppression limited the actual amount of phytoextracted Cu at high concentrations of EDDS. The maximum amount of extracted Cu was achieved by the application of 8.0 and 4.0+4.0 mmol kg-1 EDDS. The shoot Cu concentrations after EDTA application were much lower than with EDDS at the same doses. According to these experiments, EDTA does not appear to be an efficient amendment if Cu phytoextraction with B. napus is considered but EDDS is.

  13. Plasma Calcium, Inorganic Phosphate and Magnesium During Hypocalcaemia Induced by a Standardized EDTA Infusion in Cows

    Directory of Open Access Journals (Sweden)

    Enemark JMD

    2001-06-01

    Full Text Available The intravenous Na2EDTA infusion technique allows effective specific chelation of circulating Ca2+ leading to a progressive hypocalcaemia. Methods previously used were not described in detail and results obtained by monitoring total and free ionic calcium were not comparable due to differences in sampling and analysis. This paper describes a standardized EDTA infusion technique that allowed comparison of the response of calcium, phosphorus and magnesium between 2 groups of experimental cows. The concentration of the Na2EDTA solution was 0.134 mol/l and the flow rate was standardized at 1.2 ml/kg per hour. Involuntary recumbency occurred when ionised calcium dropped to 0.39 – 0.52 mmol/l due to chelation. An initial fast drop of ionized calcium was observed during the first 20 min of infusion followed by a fluctuation leading to a further drop until recumbency. Pre-infusion [Ca2+] between tests does not correlate with the amount of EDTA required to induce involuntary recumbence. Total calcium concentration measured by atomic absorption remained almost constant during the first 100 min of infusion but declined gradually when the infusion was prolonged. The concentration of inorganic phosphate declined gradually in a fluctuating manner until recumbency. Magnesium concentration remained constant during infusion. Such electrolyte responses during infusion were comparable to those in spontaneous milk fever. The standardized infusion technique might be useful in future experimental studies.

  14. Immobilization of mixed bacterial cultures degrading EDTA and isolated from Bulgarian and Czech sources

    Czech Academy of Sciences Publication Activity Database

    Ivanova, V.; Tonkova, A.; Vassileva, A.; Šafaříková, Miroslava

    2004-01-01

    Roč. 51, č. 3 (2004), s. 312-317 ISSN 0477-0250. [Food Science, Techniques and Technologies 2004. Plovdiv, 27.10.2004-29.10.2004] R&D Projects: GA AV ČR IBS6087204 Institutional research plan: CEZ:AV0Z6087904 Keywords : EDTA * degradation * immobilization Subject RIV: EE - Microbiology, Virology

  15. Efektivitas KMK dan Na2 EDTA dalam Mengabsorbsi Paparan Merkuri pada Ikan Lele (Clarias batrachus

    Directory of Open Access Journals (Sweden)

    Tuti Hartati Siregar

    2010-12-01

    Full Text Available Penelitian ini dilaksanakan untuk mengamati efektivitas karboksimetil kitosan (KMK sebagai bahan pengkelat alami dan Na2 EDTA sebagai bahan pengkelat sintetis logam berat merkuri (Hg pada ikan lele. Ikan lele yang digunakan adalah jenis lele dumbo yang diperoleh dari Bogor. Ikan dipelihara dalam kolam berukuran 380 x 150 x 60 cm 3. Air kolam sebanyak 570 L yang berisi 200 ekor ikan lele dipapar dengan Hg 60–90 ppb secara bertahap selama 1 bulan dan penggantian air kolam dilakukan setiap minggu. Sebelum pemaparan dengan Hg dilakukan, ikan lele dikondisikan pada kolam percobaan selama 1 minggu. Pada minggu ke dua ikan dipapar merkuri 60 ppb, kemudian konsentrasi merkuri dinaikkan 15 ppb setiap minggu sampai dengan minggu ke empat. Pemaparan dihentikan setelah minggu ke empat. Setelah itu ikan dipanen kemudian difilet dan dikelat dengan cara direndam dalam larutan KMK dan Na 2EDTA masing-masing pada konsentrasi 0; 0,5; 1,0; dan 1,5% selama 0, 30, 60, dan 90 menit. Perendaman dalam air digunakan sebagai kontrol terhadap perlakuan tersebut. Setiap perlakuan diulang sebanyak 3 kali. Pengamatan yang dilakukan meliputi kandungan awal dan kandungan akhir Hg setelah perlakuan perendaman. Analisis dilakukan menggunakan instrumen AAS (Perkin Elmer tipe Aanalyst800. Hasil pengamatan menunjukkan bahwa perendaman dalam KMK dan Na 2 EDTA 0,5% selama 30 menit memberikan hasil yang terbaik, dan tidak ada perbedaan antara KMK dan Na2EDTA dalam fungsinya sebagai absorben logam berat.

  16. The effect of laterally positioned flap-revised technique and 24% ethylenediaminetetraacetic acid root conditioning on root coverage: A case report

    OpenAIRE

    Singh, Jagmohan; Bharti, Vipin

    2014-01-01

    Complete root coverage is considered the true goal of treatment of gingival recession defects because only complete coverage assures recovery from the hypersensitivity and esthetic defects associated with recession areas. Previous studies have shown that the laterally positioned flap (LPF) technique or root surface biomodification yields a higher percentage of complete root coverage upon gingival recession treatment. This article highlights the use of the laterally positioned pedicle flap-rev...

  17. Purification and characterization of amine oxidase from Vigna ...

    African Journals Online (AJOL)

    user1

    2012-09-27

    Sep 27, 2012 ... volume of 50 mM potassium phosphate buffer (pH 8.0), dialyzed 3 times against ... and subsequently soaked in distilled water to stop the reaction. A duplicate ... The effects of ethylenediaminetetraacetic acid (EDTA), a metal.

  18. In Vitro Antioxidant Activity of Chitosan Aqueous Solution: Effect of ...

    African Journals Online (AJOL)

    ... (CS-TPP) and chitosan ethylenediaminetetraacetic acid (CS-EDTA) solution were determined employing various established in vitro system such as superoxide and hydroxyl radicals scavenging, metal ion chelating and reducing power. Their chemical structures were characterized by nuclear magnetic resonance (NMR) ...

  19. GLYCOHEMOGLOBIN - COMPARISON OF 12 ANALYTICAL METHODS, APPLIED TO LYOPHILIZED HEMOLYSATES BY 101 LABORATORIES IN AN EXTERNAL QUALITY ASSURANCE PROGRAM

    NARCIS (Netherlands)

    WEYKAMP, CW; PENDERS, TJ; MUSKIET, FAJ; VANDERSLIK, W

    Stable lyophilized ethylenediaminetetra-acetic acid (EDTA)-blood haemolysates were applied in an external quality assurance programme (SKZL, The Netherlands) for glycohaemoglobin assays in 101 laboratories using 12 methods. The mean intralaboratory day-to-day coefficient of variation (CV),

  20. Extracellular β-D-fructofuranosidase from Aspergillus parasiticus ...

    African Journals Online (AJOL)

    LUCCA, A. L.

    2013-09-18

    Sep 18, 2013 ... submerged fermentation was optimized by factorial design and high levels of enzyme were obtained in. 24 h-old cultures at 30°C ... electrophoresis; EDTA, ethylenediaminetetraacetic acid. ... bacteria (Awad et al., 2013), yeast (Kumar and ..... caespitosus under submerged or solid state fermentation using.

  1. Cd2+ and Zn2+ sorption on apatite in the presence of EDTA and humic substance

    Directory of Open Access Journals (Sweden)

    Viipsi K.

    2013-04-01

    Full Text Available The sorption of Cd2+ and Zn2+ on hydroxyapatite [HAP- Ca10(PO46(OH2] and fluorapatite [FAP- Ca10( PO46(F2] with different specific surface area and stoichiometry was investigated in batch experiments in the pH range 4 to 11 (25 ◦C; 0.1 M KNO3. The impact of different conditions was concerned: solution pH, the presence of complexing ligands (EDTA and humic substance and competing metal ions, as well as reaction kinetic and equilibrium conditions. To evaluate the reversibility of Cd2+ sorption onto HAP, desorption characteristics in water, Ca, EDTA, and HUM-solutions were determined. Additionally to solution analysis the surface composition of solid phases was analysed by X-Ray Photoelectron Spectroscopy XPS. The information from the chemical analyses was used to design an equilibration model that takes into account dissolution, surface potential, solution and surface complexation, as well as possible phase transformations. It was revealed that apatites effectively sorb Cd2+ and Zn2+ by ion exchange reactions on surface by formation of new surface phases. Using XPS the formation of a Me-enriched HAP surface was found, which was interpreted as the formation of a solid solution with the general formula: Ca8.4-xMex(HPO41.6(PO44.4(OH0.4. In a binary solution (Cd+Zn the competition of metals reduced individual sorbed amount compared with the single component solutions but the total adsorption maximum was approximately constant. The presence of EDTA reduces the metal sorption on apatite due to [CdEDTA]2- and [ZnEDTA]2- complexes and increases apatite solubility due to [CaEDTA]2- complex formation. The dissolved humic substance was bound on apatite in suspensions but the amount of Cd2+ bound was not changed. The results showed that the solution pH and the presence of complexing ligands have a significant effect on heavy metal sorption on apatite and must be considered if apatites are used as remediation agent. The proposed model can be used to predict

  2. Mechanism of central ion exchange in the neodymium (3) ethylenediamine-tetraacetate-copper (2) and erbium (3) ethylenediaminetetraacetate-copper (2) systems in ageous solution

    International Nuclear Information System (INIS)

    Nikitenko, S.I.; Martynenko, L.I.; Pechurova, N.I.; Spitsyn, V.I.

    1984-01-01

    The spectrophotometry method in the stationary regime and the ''Stopped Flow'' method in the prestationary regime are used to study kinetics and exchange mechanism in NdA - -Cu 2+ and ErA - -Cu 2+ (A=EDTA) systems at pH 4.0-6.0 and initial Cu 2+ concentrations (0.5-6.0)x10 -2 M, Nd 3+ , Er 3 2H+ (0-1.0)x10 -2 M. On the basis of considering the dependence of kinetic exchange characteristics on the value of initial Ln 3+ and Cu 2+ concentrations it is shown that stability constant of appearing intermediate binuclear complex [NdACu 2+ ] correlates with stability constant of copper acetate, i. e. incoming Cu 2+ cation coordinates by EDTA acetogroup at the first stage of reaction. Stability constant of [ErACu + ) correlates with outer spheric association constant, i. e. at the earlier exchange stage in the associative mechanism in the ErA - -Cu 2+ system, formation of outer spheric associate takes place. Considerable difference in exchange mechanisms for light and heavy rare earths is explained by lesser strength in metal-oxygen bond in initial NdA - as compared with ErA - . Bond strengthening in the initial rare earth complexonate prevents the formation of stable intermediate binuclear complexes which causes decrease in the exchange rate according to the associative mechanism in LuAsup(-)-Mnsup(n+) systems

  3. Roles of EDTA washing and Ca{sup 2+} regulation on the restoration of anammox granules inhibited by copper(II)

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Zheng-Zhe; Cheng, Ya-Fei; Zhou, Yu-Huang; Buayi, Xiemuguli [Department of Environmental Science and Engineering, Hangzhou Normal University, Hangzhou 310036 (China); Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036 (China); Jin, Ren-Cun, E-mail: jrczju@aliyun.com [Department of Environmental Science and Engineering, Hangzhou Normal University, Hangzhou 310036 (China); Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036 (China)

    2016-01-15

    Highlights: • 80.5% of the Cu in anammox granules was introduced via adsorption. • Cu(II) internalized on/into AnAOB cells plays a crucial role in toxicity. • EDTA washing contributes to the detoxification of anammox granules. • Ca{sup 2+} can stimulate the re-growth of damaged anammox consortium. - Abstract: We investigated the feasibility of using ethylene diamine tetraacetic acid (EDTA) washing followed by Ca{sup 2+} enhancement for the recovery of anammox reactors inhibited by Cu(II). Kinetic experiments and batch activity assays were employed to determine the optimal concentration of EDTA and washing time; and the performance and physiological dynamics were tracked by continuous-flow monitoring to evaluate the long-term effects. The two-step desorption process revealed that the Cu in anammox granules was primarily introduced via adsorption (approximately, 80.5%), and the portion of Cu in the dispersible layer was predominant (accounting for 71.1%). Afterwards, the Cu internalized in the cells (approximately, 14.7%) could diffuse out of the cells and be gradually washed out of the reactor over the next 20 days. The Ca{sup 2+} addition that followed led to an accelerated nitrogen removal rate recovery slope (0.1491 kgN m{sup −3} d{sup −2}) and a normal biomass growth rate (0.054 d{sup −1}). The nitrogen removal rate returned to normal levels within 90 days and gradual improvements in granular characteristics were also achieved. Therefore, this study provides a new insight that externally removing the adsorbed heavy metals followed by internally repairing the metabolic system may represent an optimal restoration strategy for anammox consortium damaged by heavy metals.

  4. Effectiveness of EDTA and Modified Salt Solution to Detach and Kill Cells from Enterococcus faecalis Biofilm.

    Science.gov (United States)

    de Almeida, Josiane; Hoogenkamp, Michel; Felippe, Wilson T; Crielaard, Wim; van der Waal, Suzette V

    2016-02-01

    Disruption of the matrix of endodontic biofilms will aid in their removal from a root canal. Therefore, the aim of this study was to investigate the efficacy of EDTA and a modified salt solution (MSS) to detach bacteria from biofilms. Forty-eight-hour-old Enterococcus faecalis biofilms were grown on glass coverslips and then treated for 1 hour by immersion in 17% EDTA or MSS. Phosphate-buffered saline served as a negative control. Then, residual biofilm cells on the substrate and the detached cells in the supernatant were collected. Viability was verified by the colony-forming unit (CFU) counting method. Propidium monoazide (PMA) treatment in conjunction with quantitative polymerase chain reaction (qPCR) was also performed to detect the presence of E. faecalis 16S ribonucleic RNA genes. Data were analyzed using 1-way analysis of variance and Tukey or Kruskal-Wallis and Dunn tests. The Pearson R test evaluated the correlation between results from CFU and PMA (α = 5%). qPCR showed that EDTA detached 99% of biofilm cells, and MSS detached 94% of biofilm cells (both P < .001). In contrast to EDTA, MSS was highly antimicrobial. The treatment promoted an ample log 7 reduction of the attached cells (P < .001), and almost no live cells were detected in the supernatant (P < .001). Positive correlations between CFU and qPCR with PMA were observed (r = 0.959 and r = 0.729). EDTA detached cells in biofilms with a minor antimicrobial effect. Besides a great antimicrobial effect, MSS also detached biofilm cells. These dispersals of biofilms give insights into new endodontic biofilm removal strategies. Copyright © 2016 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  5. Characterizing RecA-independent induction of Shiga toxin2-encoding phages by EDTA treatment.

    Directory of Open Access Journals (Sweden)

    Lejla Imamovic

    Full Text Available BACKGROUND: The bacteriophage life cycle has an important role in Shiga toxin (Stx expression. The induction of Shiga toxin-encoding phages (Stx phages increases toxin production as a result of replication of the phage genome, and phage lysis of the host cell also provides a means of Stx toxin to exit the cell. Previous studies suggested that prophage induction might also occur in the absence of SOS response, independently of RecA. METHODOLOGY/PRINCIPAL FINDINGS: The influence of EDTA on RecA-independent Stx2 phage induction was assessed, in laboratory lysogens and in EHEC strains carrying Stx2 phages in their genome, by Real-Time PCR. RecA-independent mechanisms described for phage λ induction (RcsA and DsrA were not involved in Stx2 phage induction. In addition, mutations in the pathway for the stress response of the bacterial envelope to EDTA did not contribute to Stx2 phage induction. The effect of EDTA on Stx phage induction is due to its chelating properties, which was also confirmed by the use of citrate, another chelating agent. Our results indicate that EDTA affects Stx2 phage induction by disruption of the bacterial outer membrane due to chelation of Mg(2+. In all the conditions evaluated, the pH value had a decisive role in Stx2 phage induction. CONCLUSIONS/SIGNIFICANCE: Chelating agents, such as EDTA and citrate, induce Stx phages, which raises concerns due to their frequent use in food and pharmaceutical products. This study contributes to our understanding of the phenomenon of induction and release of Stx phages as an important factor in the pathogenicity of Shiga toxin-producing Escherichia coli (STEC and in the emergence of new pathogenic strains.

  6. Determinação do teor de edta remanescente em soluções de limpeza de equipamentos industriais Determination of edta content in cleaning solutions after their use in industrial equipaments

    Directory of Open Access Journals (Sweden)

    Arnaldo Antonio Rodella

    2007-08-01

    Full Text Available Cleaning solutions containing EDTA are widely employed to remove incrustation despite of the costs involved. The free content of EDTA in commercial solutions may be determined by mixing an aliquot to ammonium oxalate and using a standard calcium solution as titrant. The end-point is detected by the formation of insoluble calcium oxalate after all EDTA is complexed. A system for turbidimetric detection of the end-point was envisaged to substitute the visual detection, which impaired the analyses of dark samples. The proposed method was tested with real samples and good accuracy and precision was obtained.

  7. Comparison of Three Analytical Methods for Separation of Mineral and Chelated Fraction from an Adulterated Zn-EDTA Fertilizer

    International Nuclear Information System (INIS)

    Khan, M.S.; Qazi, M.A.; Khan, N.A.; Mian, S.M.; Ahmed, N.; Ahmed, N.

    2013-01-01

    Summary: Different analytical procedures are being employed in the world to quantify the chelated portion in a Zn-EDTA fertilizer. Agriculture Department, Government of the Punjab is following Shahid's analytical method in this regard. This method is based on Ion-chromatography (IC) that separates the mineral zinc (Zn) from an adulterated Zn-EDTA fertilizer sample i.e. mixture of mineral and chelated Zn fractions. To find out its effectiveness and suitability, this comparative study was carried out by analyzing adulterated, non-adulterated Zn-EDTA standard and Zn-EDTA samples taken from market in thrice following three methods namely Shahid's (IC) analytical method, Atomic Absorption Spectrophotometric (AAS) method based on the principle of precipitating the mineral Zn fraction at high pH value by using alkali solution of suitable concentration and analysis of filtrate containing only chelated fraction and Association of Official Analytical Chemists (AOAC) method FM-841 respectively. Adulterated Zn-EDTA samples were prepared by mixing of known quantity of mineral Zn with chelated Zn-EDTA standard. The results showed that Shahid's analytical method and AAS method, both successfully estimated the chelated fraction. The AOAC FM-841 method was insensitive to put a ceiling on the mineral fraction hence did not furnish the reliable results. The Shahid's analytical method was selected being equallyeffective to produce reliable results both for solid and liquid Zn-EDTA samples. The AAS method was comparable in only liquid samples. (author)

  8. Preparation of 99mTc-EDTA-MN and Its Bioimaging in Mouse

    Directory of Open Access Journals (Sweden)

    Yongshuai QI

    2015-07-01

    Full Text Available Background and objective Hypoxia is an important biological characteristics of solid tumor, it is not sensitive to radiotherapy and chemotherapy for which is the presence of hypoxic cell, thus increasing their resistance to conventional radiotherapy and chemotherapy, therefore, the detection of hypoxia degree of tumor tissue is of great significance. The hypoxia imaging of nuclear medicine can reflect the degree of tissue hypoxia, which can selectively retained on the hypoxic cells or tissues, including nitroimidazole and non nitroimidazole; the nitroimidazole is widely and deeply researched as hypoxic celles developer in China and abroad at present. The research about application of radionuclide labelled technique has clinical application value to develop the hypoxia imaging agent EDTA-MN complexes which was labeled. To study the feasibility of 99mTc by direct labeling method, the radiochemical properties evaluation of 99mTc-EDTA-MN, and observe the distribution characteristics of 99mTc radiolabeled EDTA-MN in the xenograft lung cancer nude mice bearing non-small cell lung cancer cell (A549, and provide experimental evidence for its further research and application. Methods The radiolabeling of EDTA-MN with 99mTc was performed with direct labeling method, respectively, on the reaction dosage (10 mg, 5 mg, 2 mg, stannous chloride dosage (8 mg/mL, 4 mg/mL, 2 mg/mL, mark system pH (2, 4, 5, 6 one by one test, using orthogonal design analysis, to find the optimal labeling conditions. Labelling rate, radiochemical purity, lipid-water partition coefficient and in vitro stability in normal saline (NS were determined by TLC and HPLC, and the preliminary study on the distribution of 99mTc-EDTA-MN in nude mice. Results The labeling rate of 99mTc-EDTA-MN with the best labeling conditions was (84.11±2.83%, and the radiochemical purity was higher than 90% by HPLC purification, without any notable decomposition at room temperature over a period of 12 h. The

  9. Biological degradation of EDTA in pulping effluents at higher pH - a laboratory study

    Energy Technology Data Exchange (ETDEWEB)

    Ek, M; Remberger, M; Allard, A S

    1999-01-01

    The biological degradation of EDTA at different pH, sludge load and sludge age has been investigated in laboratory experiments. The experiments showed that relatively fast degradation of EDTA in the form found in this waste water (from production of TMP) took place at least at pH around 8.5 with moderate COD load and high sludge age. In continuous reactors the degradation of EDTA in a pulp and paper waste water was 2-3 mg EDTA/g SS*day at both pH 7 and 8,5, and at sludge ages from 5 to 21 days. The degradation was dependent on sludge load, and no degradation was seen above 1 g COD/g SS*day. In kinetic experiments with half strength waste water the same degradation rate (1,5-2 mg EDTA/g SS*day) was found at pH 7 and at pH 8,5 with sludge of low age (9 and 5 days SRT). Much faster degradation was found at pH 8,5 with sludge of high age (21 days in the continuous experiment). The mean degradation rate was over 10 mg EDTA/g SS*day from 20 to 5 mg EDTA/l. v{sub max} was determined to be 35 mg EDTA/g SS*day and K{sub M} to 31 mg EDTA/l. COD removal was at least as good at pH 8,5 as at pH 7. Sludge properties were best at pH 8,5 and long sludge retention time (giving low sludge load). Both sludge volume index and residual suspended solids after sedimentation were lower than under normal conditions at pH 7. The direct cost for caustic lime would be about 15 SEK per ton of TMP, with a water like the one investigated here. This can vary a lot depending on starting pH and buffering capacity. Costs for addition of nitrogen source could probably be omitted, but this is normally not more than 1-2 SEK per ton of TMP. The extra need for oxygen in the treatment would not be more than some percent, but may be important if oxygen is limited. A substantial extra cost would be if the aeration volume has to be increased. According to the best results from the kinetic study, this would not be needed in an extended aeration activated plant with 2 days HRT and sludge concentrations of 2

  10. PENGARUH PENGOLAHAN DAN PENAMBAHAN Na2EDTA PADA TERIGU FORTIFIKASI TERHADAP KETERSEDIAAN BIOLOGIS SENG

    Directory of Open Access Journals (Sweden)

    Estu Nugroho

    2014-05-01

    Full Text Available ABSTRACTThe objective of this research was to analyze changes in bioavailability of zinc (Zn caused by addition of Na2EDTA and processing of fortified wheat flour. Bioavailability of zinc was tested by in vitro method as a simulation of human digestive track. The result showed that there was no significant difference in the bioavailability of zinc on wheat flour which is processed by frying (donuts, steaming (steamed bun, and baking (bread (p>0.05. However, there was a tendency to increase the bioavailability of wheat flour processed by frying (donuts and baking (bread when compared to the raw dough. Raw dough had bioavailability of zinc 13.29%, after frying (donut increase to 14.72%, and after baking (bread increase to 17.06%. The addition of Na2EDTA also did not increase bioavailability of zinc significantly for the three types of processing (p>0.05, despite increased bioavailability of zinc on the donuts by 4.67%.Keywords: bioavailability, in vitro, Na2EDTA, wheat flour, zincABSTRAKTujuan penelitian ini adalah menganalisis perubahan ketersediaan biologis seng (Zn yang terjadi akibat penambahan Na2EDTA dan pengolahan terigu yang difortifikasi. Uji ketersediaan biologis seng dilakukan secara in vitro dengan mensimulasikan pencernaan manusia. Hasil uji menunjukkan bahwa tidak terdapat perbedaan ketersediaan biologis seng yang nyata pada terigu yang digoreng (donat, dikukus (bakpau, ataupun dipanggang (roti (p>0.05. Namun, terdapat kecenderungan peningkatan ketersediaan biologis pada terigu yang diolah dengan cara digoreng (donat dan dipanggang (roti dibandingkan dengan adonan mentah. Adonan mentah memiliki nilai ketersediaan biologis seng sebesar 13.29%, setelah digoreng (donat memiliki nilai ketersediaan biologis seng sebesar 14.72% dan setelah dipanggang (roti memiliki nilai ketersediaan biologis seng sebesar 17.06%. Penambahan Na2EDTA juga tidak memberikan peningkatan ketersediaan biologis seng yang nyata pada ketiga jenis pengolahan yang

  11. Mobilisation of heavy metals into the urine by CaEDTA: relation to erythrocyte and plasma concentrations and exposure indicators.

    OpenAIRE

    Araki, S; Aono, H; Murata, K

    1986-01-01

    To investigate the effects of calcium disodium ethylenediamine tetra-acetate (CaEDTA) on the urinary excretion, erythrocyte, and plasma concentrations and exposure indicators of seven heavy metals, CaEDTA was administered by intravenous infusion to 20 workers exposed to lead, zinc, and copper. The workers' blood lead concentrations ranged from 22 to 59 micrograms/dl (mean 38 micrograms/dl (1.8 mumol/l]. The 24 hour urinary excretion of metals after CaEDTA administration (mobilisation yield) w...

  12. The optimal period of Ca-EDTA treatment for parthenogenetic activation of porcine oocytes during maturation culture

    OpenAIRE

    MORITA, Yasuhiro; TANIGUCHI, Masayasu; TANIHARA, Fuminori; ITO, Aya; NAMULA, Zhao; DO, Lanh Thi Kim; TAKAGI, Mitsuhiro; TAKEMOTO, Tatsuya; OTOI, Takeshige

    2016-01-01

    The changes triggered by sperm-induced activation of oocytes, which are required for normal oocyte development, can be mediated by other agents, thereby inducing the parthenogenesis. In this study, we exposed porcine oocytes to 1 mM Ca-EDTA, a metal-ion chelator, at various intervals during 48 hr of in vitro maturation to determine the optimum period of Ca-EDTA treatment for parthenogenetic activation. When the oocytes were cultured with or without Ca-EDTA from 36 hr (post-12), 24 hr (post-24...

  13. Effect of pH on the dissolution kinetics of zero-valent iron in the presence of EDDHA and EDTA

    International Nuclear Information System (INIS)

    Lodge, Alexander M.; Pierce, Eric M.; Wellman, Dawn M.; Rodriguez, Elsa A.

    2007-01-01

    The effect of environmental factors (e.g., pH, solution composition, and temperature) that affect the longevity of Fe(0) barriers in the subsurface are difficult to quantify independently from changes that occur to the passivating layer. Therefore, to quantify the rate of Fe(0) dissolution under conditions which maintain the pO2 at a relatively constant level and minimize the formation of a passivating layer on the metal surface, a series of experiments have been conducted with the single-pass flow-through (SPFT) apparatus. These experiments were conducted over the pH range from 7.0 to 12.0 at 90 C in the presence of 5 mM EDDHA or 5 mM EDTA. The organic acids, EDDHA and EDTA, helped to maintain the aqueous Fe concentration below saturation with respect to Fe-bearing alteration phases and minimize the formation of a partially oxidized surface film. Results suggest the corrosion of Fe(0) is relatively insensitive to pH and the forward or maximum dissolution rate is 3 to 4 orders of magnitude higher than when a passive film and corrosion products are present

  14. Investigation into complexing of pentavalent actinide forms with some anions of organic acids by the coprecipitation method

    International Nuclear Information System (INIS)

    Moskvin, A.I.; Poznyakov, A.N.; AN SSSR, Moscow. Inst. Geokhimii i Analiticheskoj Khimii)

    1979-01-01

    Complexing of pentavolent forms of Np, Pu, Am actinides with anions of acetic, oxalic acids and EDTA is studied using the method of coprecipitation with iron hydroxide. Composition and stability constants of the actinide complexes formed are determined. The acids anions are arranged in a row in the order of decrease of complexing tendency that is EDTA anion>C 2 O 4 2- >CH 3 COO -

  15. Mechanism of central ion exchange in the neodymium (3) ethylenediamine-tetraacetate-copper (2) and erbium (3) ethylenediaminetetraacetate-copper (2) systems in aqueous solution

    Energy Technology Data Exchange (ETDEWEB)

    Nikitenko, S.I.; Martynenko, L.I.; Pechurova, N.I.; Spitsyn, V.I. (Moskovskij Gosudarstvennyj Univ. (USSR))

    1984-01-01

    The spectrophotometry method in the stationary regime and the ''Stopped Flow'' method in the prestationary regime are used to study kinetics and exchange mechanism in NdA/sup -/-Cu/sup 2 +/ and ErA/sup -/-Cu/sup 2 +/ (A=EDTA) systems at pH 4.0-6.0 and initial Cu/sup 2 +/ concentrations (0.5-6.0) x 10/sup -2/ M, Nd/sup 3 +/, Er/sup 3/2H+ (0-1.0) x 10/sup -2/ M. On the basis of considering the dependence of kinetic exchange characteristics on the value of initial Ln/sup 3 +/ and Cu/sup 2 +/ concentrations it is shown that stability constant of appearing intermediate binuclear complex (NdACu/sup 2 +/) correlates with stability constant of copper acetate, i.e. incoming Cu/sup 2 +/ cation coordinates by EDTA acetogroup at the first stage of reaction. Stability constant of (ErACu/sup +/) correlates with outer spheric association constant, i.e. at the earlier exchange stage in the associative mechanism in the ErA/sup -/-Cu/sup 2 +/ system, formation of outer spheric associate takes place. Considerable difference in exchange mechanisms for light and heavy rare earths is explained by lesser strength in metal-oxygen bond in initial NdA/sup -/ as compared with ErA/sup -/. Bond strengthening in the initial rare earth complexonate prevents the formation of stable intermediate binuclear complexes which causes decrease in the exchange rate according to the associative mechanism in LuAsup(-)-Mnsup(n+) systems.

  16. Bioaccumulation in Porcellio scaber (Crustacea, Isopoda) as a measure of the EDTA remediation efficiency of metal-polluted soil

    International Nuclear Information System (INIS)

    Udovic, Metka; Drobne, Damjana; Lestan, Domen

    2009-01-01

    Leaching using EDTA applied to a Pb, Zn and Cd polluted soil significantly reduced soil metal concentrations and the pool of metals in labile soil fractions. Metal mobility (Toxicity Characteristic Leaching Procedure), phytoavailability (diethylenetriaminepentaacetic acid extraction) and human oral-bioavailability (Physiologically Based Extraction Test) were reduced by 85-92%, 68-91% and 88-95%, respectively. The metal accumulation capacity of the terrestrial isopod Porcellio scaber (Crustacea) was used as in vivo assay of metal bioavailability, before and after soil remediation. After feeding on metal contaminated soil for two weeks, P. scaber accumulated Pb, Zn and Cd in a concentration dependent manner. The amounts of accumulated metals were, however, higher than expected on the basis of extraction (in vitro) tests. The combined results of chemical extractions and the in vivo test with P. scaber provide a more relevant picture of the availability stripping of metals after soil remediation. - Bioaccumulation tests with Porcellio scaber isopods are proposed as a supplement to chemical extraction in assessing metal bioavailability before and after soil remediation.

  17. Effect of EDTA and gypsum on self diffusion coefficient of zinc in alkali soil

    International Nuclear Information System (INIS)

    Gupta, G.N.; Deb, D.L.

    1981-01-01

    The effect of EDTA and gypsum application on the rate of zinc diffusion was studied in an alkali soil. Gypsum application at the rate of half gypsum requirement (GR) increased the apparent self diffusion coefficient of zinc (DaZn) and decreased the capacity factor (B) of soil. The higher rates (full GR and double GR) depressed the rate of zinc diffusion and increased the B value. Application of EDTA at the rate of 0.77 μeg -1 of soil produced 1600 and 24 fold increase in DaZn and DpZn values respectively and 100 times drop in B value. Addition of 55 ppm Zn to the soil significantly increased the DaZn and DpZn values. (author)

  18. Intestinal permeability to [51Cr]EDTA in children with cystic fibrosis

    International Nuclear Information System (INIS)

    Leclercq-Foucart, J.; Forget, P.; Sodoyez-Goffaux, F.; Zappitelli, A.

    1986-01-01

    Intestinal permeability was investigated in 14 children with cystic fibrosis making use of [ 51 Cr]EDTA as probe molecule. Ten normal young adults and 11 children served as controls. After oral administration of [ 51 Cr]EDTA, 24 h urine was collected. Urinary radioactivity was calculated and results expressed as percentage of oral dose excreted in 24 h urine. Mean and SEM were as follows: 2.51 +/- 0.21, 2.35 +/- 0.24, and 13.19 +/- 1.72 for control children, normal adults, and cystic fibrosis patients, respectively. The permeability differences between cystic fibrosis patients and either control children or control adults are significant (p less than 0.001)

  19. Use of EDTA for potentiometric back titration of rare earths and analysis of their mixtures

    International Nuclear Information System (INIS)

    Zayed, M.A.; Rizk, M.S.; Khalifa, H.; Omer, W.F.

    1987-01-01

    Advantage was taken of the stoichiometric reaction between mercury(II), rare earths, alkaline earths, heavy metal ions and EDTA in urotropine buffered media to determine rare earths by back-titration of excess EDTA in the course of estimating a variety of lanthanides or analysing their binary mixture with one of the alkaline earth metals by selective control of pH; or analysing their binary mixtures with heavy metals using fluoride as a good masking agent for rare earths; or analysing their ternary mixtures with both heavy and alkaline earth metals in two steps, one by selective control of pH and the other by masking of rare earths with fluoride at lower pH to estimate the heavy metal. The procedures given are simple, rapid and extremely reliable. 19 refs. (author)

  20. A novel biamperometric methodology for thorium determination by EDTA complexometric titration

    International Nuclear Information System (INIS)

    Jayachandran, K.; Gamare, J.S.; Nair, P.R.; Xavier, M.; Aggarwal, S.K.

    2012-01-01

    A biamperometric methodology is described for the determination of thorium by EDTA complexometric titration, based on the observed electrochemical behaviour of EDTA when the applied potential was ≥ 200 mV between the twin Pt electrodes. Studies carried out showed that a pH range of 2.4-2.7 was optimum for the determination. Accuracy and precision of the method were evaluated using different amounts of thorium ranging from 50 μg to 5 mg. Studies on the interference of uranium were carried out with different amounts of uranium ranging from 20 to 80% using the presently developed approach of biamperometry as well as the conventional indicator method. The method was employed for the determination of Th in (Th,U)O 2 samples containing different amounts of Th and U. (orig.)

  1. Structural modification of mordenite zeolite with Fe For the photo-degradation of EDTA

    International Nuclear Information System (INIS)

    Emara, Mostafa M.; Tourky, Amal S.M.; El-Moselhy, Medhat M.

    2009-01-01

    Fe 2+ was incorporated inside mordenite through ion exchange technique in aqueous solution. The amount of Fe loading was 25-100 wt %, using FeSO 4 .7H 2 O as precursor and Na-mordenite starting material Na-M. The Fe incorporated (Fe-M) thus prepared was characterized by XRD, FTIR and N 2 adsorption measurements. It was found that Fe mordenite retained the same structure as that for Na-mordenite which may indicate that Fe well dispersed into mordenite channels. BET indicated that Fe-M samples possessed higher surface area compared to the parent Na-M. Photocatalytic degradation of EDTA was carried out in presence of the prepared Fe-M catalysts. Effects of catalyst concentration and temperature were also studied. Thermodynamic parameters calculated for 50% Fe-M showed the highest catalytic activity toward EDTA degradation.

  2. Structural modification of mordenite zeolite with Fe For the photo-degradation of EDTA

    Energy Technology Data Exchange (ETDEWEB)

    Emara, Mostafa M. [Chemistry Department, Faculty of Science (boys), Al-Azha University, Nasr city 11884, Cairo (Egypt); Tourky, Amal S.M. [Chemistry Department, Faculty of Science (Girls), Al-Azhar University, Nasr city, Cairo (Egypt); El-Moselhy, Medhat M., E-mail: medhatmohamed@yahoo.com [Chemistry Department, Faculty of Science (boys), Al-Azha University, Nasr city 11884, Cairo (Egypt)

    2009-07-15

    Fe{sup 2+} was incorporated inside mordenite through ion exchange technique in aqueous solution. The amount of Fe loading was 25-100 wt %, using FeSO{sub 4}.7H{sub 2}O as precursor and Na-mordenite starting material Na-M. The Fe incorporated (Fe-M) thus prepared was characterized by XRD, FTIR and N{sub 2} adsorption measurements. It was found that Fe mordenite retained the same structure as that for Na-mordenite which may indicate that Fe well dispersed into mordenite channels. BET indicated that Fe-M samples possessed higher surface area compared to the parent Na-M. Photocatalytic degradation of EDTA was carried out in presence of the prepared Fe-M catalysts. Effects of catalyst concentration and temperature were also studied. Thermodynamic parameters calculated for 50% Fe-M showed the highest catalytic activity toward EDTA degradation.

  3. 99mTc-EDTA and 99mTc-DTPA complexes as hydrological tracers

    International Nuclear Information System (INIS)

    Dominguez, J.; Borroto, J.; Nazco, J.; Perez, E.; Gamboa, R.; Cruz, J.

    2002-01-01

    The [ 99m Tc-DTPA] 2- and [ 99m Tc-EDTA] 1- were evaluated as radiotracers for short time hydrological studies. Their complex stability after labelling with 9.25 GBq of 99m Tc, the behaviour against pH variations, from 5 to 9, in simulated solutions and in natural river waters and the sorption of these compounds on the river sediments, were tested in laboratory experiments. Finally field double tracing experiments were carried out for each of labelling complexes and Rhodamine WT. From recovery calculations not losses of the 99m Tc activity were observed. The shape of the RTD curves of the [ 99m Tc-DTPA] 2- and [ 99m Tc-EDTA] 1 were quite similar to the Rhodamine Wt ones. May be concluded that both complexes behaved conservatively on the studied environmental conditions. (author)

  4. Chromium 51 EDTA/technetium 99m MDP plasma ratio to measure total skeletal function

    Energy Technology Data Exchange (ETDEWEB)

    Nisbet, A.P.; Edwards, S.; Lazarus, C.R.; Malamitsi, J.; Maisey, M.N.; Mashiter, G.D.; Winn, P.J. (Guy' s Hospital, London (UK))

    1984-08-01

    A method is described for the quantitation of total skeletal activity during bone scans. The method requires a single plasma sample only, taken at the time of imaging. The ratio of % injected dose of /sup 51/Cr EDTA to that of /sup 99/Tcsup(m) MDP is calculated from this sample following combined injection of the two radiopharmaceuticals. The /sup 51/Cr EDTA level corrects for the glomerular filtration of /sup 99/Tcsup(m) MDP. Using this method, which only requires a gamma counter, significant differences from normal controls have been shown in patients with osteomalacia, renal osteodystrophy, Paget's disease and hypercalcaemia. The method provides routine quantitative data to add to the imaging information in the bone scan.

  5. Field and laboratory evaluation of the mobility of cobalt-60/EDTA in an arid environment

    International Nuclear Information System (INIS)

    Jones, T.L.; Gee, G.W.; Swanson, J.L.; Kirkham, R.R.

    1983-02-01

    The ability of the organic complexant EDTA to enhance the mobility of cobalt-60 was investigated in both laboratory and field experiments. Laboratory tests consisted of short term (approximately 7 day) column and batch adsorption tests using soil from the Hanford site as well as long term (approximately 70 day) batch tests with Hanford soil and soils from Oak Ridge and Savannah River. In addition, two large scale tracer tests were conducted using Hanford soil. One used a large (1.6 m) laboratory column, spiked with cobalt-60/EDTA and the other was a field test conducted in an 8 m deep lysimeter. Enhanced mobility decreased sorption were observed in both column and batch tests when the cobalt-60/EDTA solutions contacted Hanford and Oak Ridge soil for only a few days. When long contact times were allowed (months) the Hanford soil showed large increases in sorption with time. The low sorption exhibited initially by the Oak Ridge soil increased slightly over time, however, the high sorption observed with the Savannah River soil remained constant with time. The reduced mobility, with time, observed in Hanford soils was confirmed in both the large scale laboratory and breaking down when contacted with Hanford and Savannah River soil and to a lesser extent, the Oak Ridge soil. It is not known at this time why the complex is breaking down or why the kinetics are different among the soils tested. The implication to waste management is that the potential for transport of cobalt by EDTA complexation may not be as serious as once thought

  6. Design of Ion-Exchange Resins Through EDTA and DTPA Modified Ligands

    Directory of Open Access Journals (Sweden)

    2014-07-01

    Catechol, resorcinol, and their admixtures with EDTA and DTPA moieties were converted into polymeric resins by alkaline polycondensation with formaldehyde. The resins were characterized by FTIR spectroscopy, elemental analysis, ion-exchange capacity, and distribution coefficient (D for heavy metal and radionuclide such as Cs and Sr. 137Cs and 90Sr constitutes a major source of heat in nuclear waste streams and in regards to recent nuclear event their remediation in complex solution – sea water - represent an important issue.

  7. Effects of EDTA on phytoextraction of heavy metals (Zn, Mn and Pb) from sludge-amended soil with Brassica napus.

    Science.gov (United States)

    Zaier, Hanen; Ghnaya, Tahar; Ben Rejeb, Kilani; Lakhdar, Abdelbasset; Rejeb, Salwa; Jemal, Fatima

    2010-06-01

    Sludge application is a reliable practice to ameliorate soil fertility. However, repetitive sludge addition represents a potential soil contamination source with heavy metals, which must be extracted. The aim of this study was to evaluate the capacity of Brassica napus to remove metals from soils amended with sludge, and to study the effect of EDTA on this process. Seedlings were cultivated in presence of sludge combined or not with EDTA. Results showed that sludge ameliorate significantly biomass production. This effect was accompanied with an increase in Pb, Zn and Mn shoot concentrations. EDTA application does not affect significantly plant growth. However, this chelator enhances shoot metals accumulation. It's therefore concluded that sludge has a beneficial effect on soil fertility, B. napus can be used for the decontamination of affected soils and that the EDTA addition increases the ability of B. napus to accumulate heavy metals. Published by Elsevier Ltd.

  8. Incorporation of rare-earth ions in Mg-Al layered double hydroxides: intercalation with an [Eu(EDTA)] - chelate

    Science.gov (United States)

    Li, Cang; Wang, Ge; Evans, David G.; Duan, Xue

    2004-12-01

    Reaction of an aqueous slurry of an Mg 2Al-NO 3 layered double hydroxide with a four-fold excess of Na[Eu(EDTA)] gives a material which analyses for Mg 0.68Al 0.32(OH) 2[Eu(EDTA)] 0.10(CO 3) 0.11·0.66H 2O. The interlayer spacing of the material is 13.8 Å, corresponding to a gallery height of 9.0 Å, which accords with the maximal dimensions (9-10 Å) of the anion in metal-EDTA complex salts as determined by single crystal X-ray diffraction. Geometrical considerations show that the charge density on the layered double hydroxide layers is too high to be balanced by intercalation of [Eu(EDTA)] - alone, necessitating the co-intercalation of carbonate ions which have a much higher charge density.

  9. In vitro antimicrobial activity of a commercial ear antiseptic containing chlorhexidine and Tris-EDTA.

    Science.gov (United States)

    Guardabassi, Luca; Ghibaudo, Giovanni; Damborg, Peter

    2010-06-01

    Minimum bactericidal concentrations (MBCs) of a commercial ear antiseptic containing chlorhexidine 0.15% and Tris-EDTA (Otodine) were determined by broth microdilution for 150 isolates representing the most common pathogens associated with canine otitis. The microorganisms were classified into three groups according to their levels of susceptibility. The most susceptible group included Staphylococcus pseudintermedius, Malassezia pachydermatis, Streptococcus canis and Corynebacterium auriscanis, which were generally killed by 1 : 64 dilution of the antiseptic product (MBC = 23/0.8 microg/mL of chlorhexidine/Tris-EDTA). The most resistant organism was Proteus mirabilis, which survived up to 1 : 8 dilution of the product (MBC = 375/12 microg/mL). Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus displayed intermediate MBCs ranging between 188/6 and 47/1.5 microg/mL. Interestingly, S. pseudintermedius was more susceptible than S. aureus, and no significant difference was observed between meticillin-resistant and meticillin-susceptible isolates within each species, indicating that antiseptic use is unlikely to co-select for meticillin resistance. Although the concentrations required for killing (MBCs) varied considerably with microorganism type, the combination of chlorhexidine 0.15% and Tris-EDTA was active against all the pathogens most commonly involved in canine otitis.

  10. Preparation and radiopharmaceutical control of 169Yb EDTA an agent for kidney function and scintigraphy

    International Nuclear Information System (INIS)

    Gulbaba, G.; Ozker; Tomek, F.

    1976-01-01

    169 Yb was produced by thermal neutron irradiation of Yb 2 O 3 in 1 MW research reactor at Cekmece Nuclear Research Center. 169 Yb-EDTA complex was then prepared with a sodium salt of EDTA. Radionuclidic and radiochemical purities of the compound were determined by gama spectral analysis and radiochromatography-electrophoresis following preparation. Ionic Yb(III) which accumulates at bone and liver was not observed on the radiochromatographic and electrophoretic analysis of the final compound. There was no separation of the label for two months of examination in order to determine stability of the compound. In conclusion, the labeled compound has been prepared for use in the external scanning of kidney and determining the glomerular filtration rate. The label appears to be firmly bound so that the agent can be stored for a reasonably long period as 31 days half-life of 169 Yb permits. Administration of the compound is safe from the stand point of radiation dose since a 30 micro-Ci 169 Yb-EDTA for a glomerular filtration study delivers no more than 0.4 mrad whole-body and 5 mrad kidney dose. (author)

  11. Iron Fortified Complementary Foods Containing a Mixture of Sodium Iron EDTA with Either Ferrous Fumarate or Ferric Pyrophosphate Reduce Iron Deficiency Anemia in 12- to 36-Month-Old Children in a Malaria Endemic Setting: A Secondary Analysis of a Cluster-Randomized Controlled Trial.

    Science.gov (United States)

    Glinz, Dominik; Wegmüller, Rita; Ouattara, Mamadou; Diakité, Victorine G; Aaron, Grant J; Hofer, Lorenz; Zimmermann, Michael B; Adiossan, Lukas G; Utzinger, Jürg; N'Goran, Eliézer K; Hurrell, Richard F

    2017-07-14

    Iron deficiency anemia (IDA) is a major public health problem in sub-Saharan Africa. The efficacy of iron fortification against IDA is uncertain in malaria-endemic settings. The objective of this study was to evaluate the efficacy of a complementary food (CF) fortified with sodium iron EDTA (NaFeEDTA) plus either ferrous fumarate (FeFum) or ferric pyrophosphate (FePP) to combat IDA in preschool-age children in a highly malaria endemic region. This is a secondary analysis of a nine-month cluster-randomized controlled trial conducted in south-central Côte d'Ivoire. 378 children aged 12-36 months were randomly assigned to no food intervention ( n = 125; control group), CF fortified with 2 mg NaFeEDTA plus 3.8 mg FeFum for six days/week ( n = 126; FeFum group), and CF fortified with 2 mg NaFeEDTA and 3.8 mg FePP for six days/week ( n = 127; FePP group). The outcome measures were hemoglobin (Hb), plasma ferritin (PF), iron deficiency (PF anemia (Hb iron deficiency with or without anemia ( p = 0.068). IDA prevalence sharply decreased in the FeFum (32.8% to 1.2%, p anemia. These data indicate that, despite the high endemicity of malaria and elevated inflammation biomarkers (C-reactive protein or α-1-acid-glycoprotein), IDA was markedly reduced by provision of iron fortified CF to preschool-age children for 9 months, with no significant differences between a combination of NaFeEDTA with FeFum or NaFeEDTA with FePP. However, there was no overall effect on anemia, suggesting most of the anemia in this setting is not due to ID. This trial is registered at clinicaltrials.gov (NCT01634945).

  12. Using electrocoagulation for metal and chelant separation from washing solution after EDTA leaching of Pb, Zn and Cd contaminated soil.

    Science.gov (United States)

    Pociecha, Maja; Lestan, Domen

    2010-02-15

    Electrocoagulation with an Al sacrificial anode was tested for the separation of chelant and heavy metals from a washing solution obtained after leaching Pb (3200 mg kg(-1)), Zn (1100 mg kg(-1)), and Cd (21 mg kg(-1)) contaminated soil with EDTA. In the electrochemical process, the sacrificial anode corroded to release Al(3+) which served as coagulant for precipitation of chelant and metals. A constant current density of 16-128 mAc m(-2) applied between the Al anode and the stainless-steel cathode removed up to 95% Pb, 68% Zn and 66% Cd from the soil washing solution. Approximately half of the initial EDTA remained in the washing solution after treatment, up to 16.3% of the EDTA was adsorbed on Al coagulant and precipitated, the rest of the EDTA was degraded by anodic oxidation. In a separate laboratory-scale remediation experiment, we leached a soil with 40 mmol EDTA per kg of soil and reused the washing solution (after electrocoagulation) in a closed loop. It removed 53% of Pb, 26% of Zn and 52% of Cd from the soil. The discharge solution was clear and colourless, with pH 7.52 and 170 mg L(-1) Pb, 50 mg L(-1) Zn, 1.5 mg L(-1) Cd and 11 mM EDTA.

  13. Effect of order of draw of blood samples during phlebotomy on routine biochemistry results.

    Science.gov (United States)

    Sulaiman, Raashda A; Cornes, Michael P; Whitehead, Simon J; Othonos, Nadia; Ford, Clare; Gama, Rousseau

    2011-11-01

    To investigate whether incorrect order of draw of blood samples during phlebotomy causes in vitro potassium ethylenediaminetetraacetic acid EDTA (kEDTA) contamination of blood samples. Serum kEDTA, potassium, calcium, magnesium, alkaline phosphatase, zinc and iron concentrations were measured in blood samples drawn before and after collecting blood into kEDTA containing sample tubes by an experienced phlebotomist using the Sarstedt Safety Monovette system. EDTA was undetectable in all samples. The concentrations of other analytes were similar in blood samples drawn before and after collection of the EDTA blood sample. Order of draw of blood samples using the Sarstedt Safety Monovette system has no effect on serum biochemistry results, when samples are taken by an experienced phlebotomist.

  14. Determination of vanadium(V) by direct automatic potentiometric titration with EDTA using a chemically modified electrode as a potentiometric sensor.

    Science.gov (United States)

    Quintar, S E; Santagata, J P; Cortinez, V A

    2005-10-15

    A chemically modified electrode (CME) was prepared and studied as a potentiometric sensor for the end-point detection in the automatic titration of vanadium(V) with EDTA. The CME was constructed with a paste prepared by mixing spectral-grade graphite powder, Nujol oil and N-2-naphthoyl-N-p-tolylhydroxamic acid (NTHA). Buffer systems, pH effects and the concentration range were studied. Interference ions were separated by applying a liquid-liquid extraction procedure. The CME did not require any special conditioning before using. The electrode was constructed with very inexpensive materials and was easily made. It could be continuously used, at least two months without removing the paste. Automatic potentiometric titration curves were obtained for V(V) within 5 x 10(-5) to 2 x 10(-3)M with acceptable accuracy and precision. The developed method was applied to V(V) determination in alloys for hip prosthesis.

  15. Chelating agents improve enzymatic solubilization of pectinaceous co-processing streams

    DEFF Research Database (Denmark)

    Ravn, Helle Christine; Meyer, Anne S.

    2014-01-01

    of different levels of ethylene-diaminetetraacetic acid (EDTA), citric acid, oxalic acid, and phosphate was assessed in relation to enzymatic solubilization of isopropanol precipitatable oligo- and polysaccharides from sugar beet pulp, citrus peel, and two types of potato pulp. The two types of potato pulp...... solubilization yields. The effect of the chelating agents correlated to their dissociation constants (pKa values) and calcium binding constants and citric acid and EDTA exerted highest effects. Maximum polysaccharide yield was obtained for FiberBind 400 where the enzymatic treatment in presence of citric acid...

  16. Insights into cadmium induced physiological and ultra-structural disorders in Juncus effusus L. and its remediation through exogenous citric acid

    International Nuclear Information System (INIS)

    Najeeb, Ullah; Jilani, Ghulam; Ali, Shafaqat; Sarwar, Muhammad; Xu Ling; Zhou, Weijun

    2011-01-01

    This study appraised cadmium (Cd) toxicity stress in wetland plant Juncus effusus, and explored its potential for Cd phytoextraction through chelators (citric acid and EDTA). Cadmium altered morphological and physiological attributes of J. effusus as reflected by growth retardation. Citric acid in the presence of 100 μM Cd significantly countered Cd toxicity by improving plant growth. Elevated Cd concentrations reduced translocation factor that was increased under application of both chelators. Citric acid enhanced Cd accumulation, while EDTA reduced its uptake. Cadmium induced oxidative stress modified the antioxidative enzyme activity. Both levels of citric acid (2.5 and 5.0 mM) and lower EDTA concentration (2.5 mM) helped plants to overcome oxidative stress by enhancing their antioxidative enzyme activities. Cadmium damaged the root cells through cytoplasmic shrinkage and metal deposition. Citric acid restored structure and shape of root cells and eliminated plasmolysis; whereas, EDTA exhibited no positive effect on it. Shoot cells remained unaffected under Cd treatment alone or with citric acid except for chloroplast swelling. Only EDTA promoted starch accumulation in chloroplast reflecting its negative impact on cellular structure. It concludes that Cd and EDTA induce structural and morphological damage in J. effusus; while, citric acid ameliorates Cd toxicity stress.

  17. Insights into cadmium induced physiological and ultra-structural disorders in Juncus effusus L. and its remediation through exogenous citric acid

    Energy Technology Data Exchange (ETDEWEB)

    Najeeb, Ullah [Institute of Crop Science, Zhejiang University, Hangzhou 310029 (China); Crop Sciences Institute, National Agriculture Research Centre, Islamabad 45500 (Pakistan); Jilani, Ghulam, E-mail: jilani@uaar.edu.pk [Department of Soil Science, PMAS Arid Agriculture University, Rawalpindi, Punjab 46300 (Pakistan); Ali, Shafaqat [Institute of Crop Science, Zhejiang University, Hangzhou 310029 (China); Sarwar, Muhammad [Land Resources Research Institute, National Agriculture Research Centre, Islamabad 45500 (Pakistan); Xu Ling [Institute of Crop Science, Zhejiang University, Hangzhou 310029 (China); Zhou, Weijun, E-mail: wjzhou@zju.edu.cn [Institute of Crop Science, Zhejiang University, Hangzhou 310029 (China)

    2011-02-15

    This study appraised cadmium (Cd) toxicity stress in wetland plant Juncus effusus, and explored its potential for Cd phytoextraction through chelators (citric acid and EDTA). Cadmium altered morphological and physiological attributes of J. effusus as reflected by growth retardation. Citric acid in the presence of 100 {mu}M Cd significantly countered Cd toxicity by improving plant growth. Elevated Cd concentrations reduced translocation factor that was increased under application of both chelators. Citric acid enhanced Cd accumulation, while EDTA reduced its uptake. Cadmium induced oxidative stress modified the antioxidative enzyme activity. Both levels of citric acid (2.5 and 5.0 mM) and lower EDTA concentration (2.5 mM) helped plants to overcome oxidative stress by enhancing their antioxidative enzyme activities. Cadmium damaged the root cells through cytoplasmic shrinkage and metal deposition. Citric acid restored structure and shape of root cells and eliminated plasmolysis; whereas, EDTA exhibited no positive effect on it. Shoot cells remained unaffected under Cd treatment alone or with citric acid except for chloroplast swelling. Only EDTA promoted starch accumulation in chloroplast reflecting its negative impact on cellular structure. It concludes that Cd and EDTA induce structural and morphological damage in J. effusus; while, citric acid ameliorates Cd toxicity stress.

  18. Removal of heavy metals from contaminated soil by electrodialytic remediation enhanced with organic acids.

    Science.gov (United States)

    Merdoud, Ouarda; Cameselle, Claudio; Boulakradeche, Mohamed Oualid; Akretche, Djamal Eddine

    2016-11-09

    The soil from an industrial area in Algeria was contaminated with Cr (8370 mg kg -1 ), Ni (1135 mg kg -1 ) and zinc (1200 mg kg -1 ). The electrodialytic remediation of this soil was studied using citric acid and EDTA as facilitating agents. 0.1 M citric acid or EDTA was added directly to the soil before it was introduced in an electrodialytic cell in an attempt to enhance the heavy metal solubility in the interstitial fluid. The more acidic pH in the soil when citric acid was used as the facilitating agent was not enough to mobilize and remove the metals from the soil. Only 7.2% of Ni and 6.7% of Zn were removed from the soil in the test with citric acid. The best results were found with EDTA, which was able to solubilize and complex Zn and Ni forming negatively charged complexes that were transported and accumulated in the anolyte. Complete removal was observed for Ni and Zn in the electrodialytic treatment with EDTA. Minor amounts of Cr were removed with both EDTA and citric acid.

  19. Laboratory studies into the use of the scandium-46-EDTA complex as a tracer for groundwater flow

    International Nuclear Information System (INIS)

    Cheng, H.; Nixon, S.C.

    1988-01-01

    Gamma ray emitting metal radionuclides, when complexed with an appropriate complexing agent, provide a wide choice of water tracers particularly for groundwater studies where a radionuclide of appropriate half-life suited to the particular study can be selected. Scandium-46 has easily detectable gamma emission and a suitable half-life (84 days) for medium term studies. It has been widely and successfully used as a tracer in studies of sediment movement but has not yet been introduced as a groundwater tracer. In our experiments the chemical aspects of the preparation of Sc-46-EDTA were studied in some detail and its behaviour in various mineralogical environments was evaluated with reference to the standard tracer, tritiated water. The experimental results have shown that the scandium cation can be easily complexed with EDTA to form soluble SC-EDTA. The complex is very stable in a wide range of pH; the adsorptive properties of Sc-EDTA in the batch studies and the retardation and recovery in the column tests in comparison with tritiated water are quite satisfactory. In general Sc-46-EDTA is a promising tracer for groundwater studies. In the report the appropriate conditions, procedures and some rational and efficient methods for testing the purity of Sc-46-EDTA in the preparation of the tracer solution of Sc-46-EDTA are described. In addition, it has been found that the formation of the metal hydroxide colloids is the major reason for the great loss in groundwater aquifers of most trivalent metal nuclide tracers in the cationic form including scandium-46. (author). 29 refs, 11 figs, 12 tabs

  20. Smear layer removal capacity of disinfectant solutions used with and without EDTA for the irrigation of canals: a SEM study

    Directory of Open Access Journals (Sweden)

    Menezes Ana Carolina Silveira Cardoso de

    2003-01-01

    Full Text Available The purpose of this study was to carry out a scanning electron microscopic (SEM analysis of the cleaning qualities and smear layer removal from root canal walls, instrumented and irrigated with 2.5% NaOCl, 2.0% chlorhexidine and saline solutions. Fifty extracted teeth were used in this study. All teeth were radiographed to determine the existence of a single canal. The crowns were cut at the cervical limit and the root canals were instrumented with K-type files up to size 45. During root canal preparation, irrigations were made with the different solutions being evaluated: Group 1: 2.5% NaOCl (10 roots; Group 2: 2.5% NaOCl and 17% EDTA for 2 minute (10 roots; Group 3: 2.0% chlorhexidine (10 roots; Group 4: 2.0% chlorhexidine and 17% EDTA for 2 minutes (10 roots; Group 5: saline solution (5 roots; Group 6: saline solution and 17% EDTA for 2 minutes (5 roots. After instrumentation, the canals were irrigated with each one of the solutions and the roots were cut in the buccolingual direction for SEM analysis, at the cervical, middle and apical thirds, to ascertain the presence or absence of smear layer and debris. SEM analysis was performed by three calibrated examiners and scores were submitted to Kruskal-Wallis test at the significance level of p = 5%. Results showed that the use of 17% EDTA decreased the smear layer significantly (p < 0.05 for all evaluated solutions in all thirds. When EDTA was not used, a significantly higher quantity of smear layer on the apical third was observed only in the NaOCl groups. The use of 17% EDTA was significant for debris removal except for the chlorhexidine groups. The following conclusion could be drawn: the use of 17% EDTA was necessary to enhance cleanness of the root canals.

  1. Restricted diffusion of CrEDTA and cyanocobalamine across the exchange vessels in rat hindquarters.

    Science.gov (United States)

    Haraldsson, B; Rippe, B

    1986-07-01

    The degree of diffusional restriction of skeletal muscle capillary walls to small solutes was estimated from the permeability surface area products (PS) of CrEDTA (MW = 341) and cyanocobalamine (MW = 1355), using computerized 'on-line' recordings of venous single injection indicator dilution curves. Experiments were performed on isolated perfused maximally vasodilated rat hindquarters during largely isogravimetric conditions and the arrangements allowed for measurements of capillary filtration coefficients (CFC). Extraction of tracer varied markedly as a function of transit time and, furthermore, PS increased with increasing flows, both these phenomena indicating tissue and flow heterogeneity. At maximal flows the disturbing influence of heterogeneity will be minimal and hence the diffusion capacities obtained by extrapolating PS area to infinite flows, so called PS tot values, were considered to give the best estimation of the 'true' capillary diffusion capacities. The value of PS tot was 12.9 +/- 0.5 for CrEDTA and 5.1 +/- 0.3 ml min-1 per 100 g for vitamin B12. The calculated PS tot ratio of 2.59 +/- 0.11 indicates restricted diffusion through equivalent pores of radius 53 A, whereas the ratio of the free diffusion coefficients for these solutes is 1.79. Using PS peak for the calculations (totally neglecting heterogeneity) the pore radius was, however, markedly overestimated. Thus, for a PS-ratio of 1.89 +/- 0.04 for CrEDTA vs. B12 an equivalent pore radius of 300 A was calculated. Also, using PS area (only partly correcting for heterogeneity) overestimated the pore radius (70 A) from a mean PS-ratio of 2.33 +/- 0.05. It was concluded that the equivalent pore radius in rat hindquarter microvascular walls is 53 A or even smaller in essential agreement with data from osmotic transient experiments in the same preparation (r approximately 40 A).

  2. Optimizing anti-coking abilities of zeolites by ethylene diamine tetraacetie acid modification on catalytic fast pyrolysis of corn stalk

    Science.gov (United States)

    Zhang, Bo; Zhong, Zhaoping; Song, Zuwei; Ding, Kuan; Chen, Paul; Ruan, Roger

    2015-12-01

    In order to minimize coke yield during biomass catalytic fast pyrolysis (CFP) process, ethylene diamine tetraacetie acid (EDTA) chemical modification method is carried out to selectively remove the external framework aluminum of HZSM-5 catalyst. X-ray diffraction (XRD), nitrogen (N2)-adsorption and ammonia-temperature programmed desorption (NH3-TPD) techniques are employed to investigate the porosity and acidity characteristics of original and modified HZSM-5 samples. Py-GC/MS and thermo-gravimetric analyzer (TGA) experiments are further conducted to explore the catalytic effect of modified HZSM-5 samples on biomass CFP and to verify the positive effect on coke reduction. Results show that EDTA treatment does not damage the crystal structure of HZSM-5 zeolites, but leads to a slight increase of pore volume and pore size. Meanwhile, the elimination of the strong acid peak indicates the dealumination of outer surface of HZSM-5 zeolites. Treatment time of 2 h (labeled EDTA-2H) is optimal for acid removal and hydrocarbon formation. Among all modified catalysts, EDTA-2H performs the best for deacidification and can obviously increase the yields of positive chemical compositions in pyrolysis products. Besides, EDTA modification can improve the anti-coking properties of HZSM-5 zeolites, and EDTA-2H gives rise to the lowest coke yield.

  3. Small bowel permeability to 51Cr-EDTA in children with recurrent abdominal pain

    International Nuclear Information System (INIS)

    Meer, S.B. van der.; Forget, P.P.

    1990-01-01

    Small bowel permeability was investigated in 87 children with recurrent abdominal pain by measuring the 24-h urinary excretion of orally administered 51 Cr-EDTA. The mean excreation was 3.64% ± 1.49% per 24 h. The difference between the mean urinary excretion in children with recurrent abdominal pain and control children (2.51% ± 0.70%), was significant (p<0.01, two sample t-test). The increased small permeability in children with recurrent abdominal pain might indicate an intestinal etiology for the patients complaints

  4. Positron emission tomography with 68Ga-EDTA in the diagnosis and localization of CSF fistulas

    International Nuclear Information System (INIS)

    Bergstrand, G.; Bergstroem, M.; Eriksson, L.; Edner, G.; Widen, L.

    1982-01-01

    Five patients with cerebrospinal fluid (CSF) rhinorrhea were investigated with computed tomography (CT) and positron emission tomography (PET) to localize the site of a CSF fistula. After intrathecal injection of 10 MBq of 68 Ga-EDTA, radioactivity was demonstrated in the basal cisterns. In three cases, the site of the fistula was visualized with PET. It is not always possible to demonstrate a CSF leakage with CT cisternography (CTC) using metrizamide, particularly in cases with minute fistulas or intermittent CSF rhinorrhea. With further experience and improved PET techniques, it may be possible to detect even very small fistulas

  5. The effect of CaNa{sub 2}EDTA on excretion of {sup 210}PB, {sup 210}Po and stable lead in cases of chronic lead intoxication

    Energy Technology Data Exchange (ETDEWEB)

    Knezevic, I.; Milacic, S.; Novak, Lj.; Uzelac, V.; Nesic, V

    1998-07-01

    The study describes the effect of CaNa{sub 2}EDTA on the excretion of the naturally occurring radionuclides {sup 210}Pb, {sup 210}Po and stable lead in workers' urine. The CaNa{sub 2}EDTA was administered intravenously to workers in the morning for five days. The results show that CaNa{sub 2}EDTA mobilises biologically active {sup 210}Pb, {sup 210}Po and stable lead, appreciably increasing their concentration in urine. (author)

  6. The EDTA effect on phytoextraction of single and combined metals-contaminated soils using rainbow pink (Dianthus chinensis).

    Science.gov (United States)

    Lai, Hung-Yu; Chen, Zueng-Sang

    2005-08-01

    Rainbow pink (Dianthus chinensis), a potential phytoextraction plant, can accumulate high concentrations of Cd from metal-contaminated soils. The soils used in this study were artificially added with different metals including (1) CK: original soil, (2) Cd-treated soil: 10 mg Cd kg(-1), (3) Zn-treated soil: 100 mg Zn kg(-1), (4) Pb-treated soil: 1000 mg Pb kg(-1), (5) Cd-Zn-treated soil: 10 mg Cd kg(-1) and 100 mg Zn kg(-1), (6) Cd-Pb-treated soil: 10 mg Cd kg(-1) and 1000 mg Pb kg(-1), (7) Zn-Pb-treated soil: 100 mg Zn kg(-1) and 1000 mg Pb kg(-1), and (8) Cd-Zn-Pb-treated soil: 10 mg Cd kg(-1), 100 mg Zn kg(-1), and 1000 mg Pb kg(-1). Three concentrations of 2Na-EDTA solutions (0 (control), 2, and 5 mmol kg(-1) soil) were added to the different metals-treated soils to study the influence of applied EDTA on single and combined metals-contaminated soils phytoextraction using rainbow pink. The results showed that the Cd, Zn, Pb, Fe, or Mn concentrations in different metals-treated soil solutions significantly increased after applying 5 mmol EDTA kg(-1) (p<0.05). The metal concentrations in different metals-treated soils extracted by deionized water also significantly increased after applying 5 mmol EDTA kg(-1) (p<0.05). Because of the high extraction capacity of both 0.005 M DTPA (pH 5.3) and 0.05 M EDTA (pH 7.0), applying EDTA did not significantly increase the Cd, Zn, or Pb concentration in both extracts for most of the treatments. Applying EDTA solutions can significantly increase the Cd and Pb concentrations in the shoots of rainbow pink (p<0.05). However, this was not statistically significant for Zn because of the low Zn concentration added into the contaminated soils. The results from this study indicate that applying 5 mmol EDTA kg(-1) can significantly increase the Cd, Zn, or Pb concentrations both in the soil solution or extracted using deionized water in single or combined metals-contaminated soils, thus increasing the accumulated metals concentrations in

  7. Toxicity, tissue distribution and excretion of 46ScCl3 and 46Sc-EDTA in mice

    International Nuclear Information System (INIS)

    Lachine, E.E.; Noujaim, A.A.; Ediss, C.; Wiebe, L.I.

    1976-01-01

    The acute toxicity, differential distribution in tissue, and elimination of ScCl 3 , 46 ScCl 3 , Sc-EDTA and 46 Sc-EDTA, in mice, has been investigated. The LD 50 sup(24hr) doses for ScCl 3 were 440 and 24 mg kg -1 respectively after intraperitioneal and intravenous injection, and 720 and 108 mg kg -1 respectively for Sc-EDTA. 46 ScCl 3 was extensively deposited in the liver and the spleen. 46 Sc-EDTA was rapidly taken up by the kidney with subsequent elimation via the urine. While-body desaturation kinetics for 46 Sc-EDTA were found to fit a three compartmental model. The fast elimination phase (T1/2 = 12.75 min; K = 0.05540 min -1 ) accounted for 74.6% of the dose; the intermediate phase (T1/2 = 40.2 min; K = 0.01722 min -1 ) for 21.8%, and the slow (T1/2 = 5351 min; K = 0.00013 min -1 ) for 3.6% of the dose. (author)

  8. Clinical study of renoscintiphoto using sup(99m)Tc-EDTA, -DTPA and 131I-hippuran

    International Nuclear Information System (INIS)

    Ishibashi, Akira; Ishii, Katsumi; Yoda, Kazushige; Hashimoto, Shozo

    1975-01-01

    The scintillation photographs of the kidneys of 35 cases were taken using sup(99m)Tc-EDTA. The photographs were compared with those taken using sup(99m)Tc-DTPA and 131 I-hippuran. In normal cases, sup(99m)Tc-EDTA which is excreted most slowly serves to take best the pictures of the parenchyma and the pelvis of the kidney. Being a sup(99m)Tc-labelled compound with a short half life time and being administrable at a substantial dose of 2-4mCi, sup(99m)Tc-EDTA provides an advantage to take continuous pictures of the blood vessel phase. This reagent can, therefore, help take a clear picture of the congestion site of the renal infarct. However, the renal dynamic test using EDTA or DTPA is difficult to catch such delicate changes as very slight rejection of the transplanted kidney or as very slight disturbance of the kidney parenchyma. In this respect, 131 I-hippuran is superior to EDTA and DTPA. In obstructive disorders of the kidney, there was no remarkable difference among these three reagents. (auth.)

  9. EDDS and EDTA-enhanced phytoextraction of metals from artificially contaminated soil and residual effects of chelant compounds

    Energy Technology Data Exchange (ETDEWEB)

    Luo Chunling [Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong (China); Shen Zhenguo [Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong (China); College of Life Sciences, Nanjing Agricultural University, Nanjing 210095 (China); Lou Laiqing [College of Life Sciences, Nanjing Agricultural University, Nanjing 210095 (China); Li Xiangdong [Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong (China)]. E-mail: cexdli@polyu.edu.hk

    2006-12-15

    The potential of 18 different plants to be used in the chemically enhanced phytoextraction of Cu, Pb, Zn and Cd was assessed using pot experiments. Chrysanthemum coronarium L. was the species most sensitive to the application of EDTA, and had the highest enhancement of Cu and Pb concentrations in its shoots. Compared with EDTA, EDDS was more effective in enhancing the concentration of Cu in the shoots of Chrysanthemum coronarium L. and Zea mays L. grown on multi-metal contaminated soils. The EDTA-treated soil still had a significant ability to enhance the concentrations of Cu and Pb in the shoots of Zea mays L. six months after the chelant treatment. However, the EDDS-treated soil did not have any effect in enhancing the concentrations of metals in the shoots of Zea mays L. in the second crop test. The results may indicate that EDDS biodegrades more rapidly than EDTA in soil and is better in limiting potential metal leaching. - Chrysanthemum coronarium L. was the most sensitive species to the application of chelants, and EDDS biodegrades much more rapidly than EDTA in soil.

  10. EDDS and EDTA-enhanced phytoextraction of metals from artificially contaminated soil and residual effects of chelant compounds

    International Nuclear Information System (INIS)

    Luo Chunling; Shen Zhenguo; Lou Laiqing; Li Xiangdong

    2006-01-01

    The potential of 18 different plants to be used in the chemically enhanced phytoextraction of Cu, Pb, Zn and Cd was assessed using pot experiments. Chrysanthemum coronarium L. was the species most sensitive to the application of EDTA, and had the highest enhancement of Cu and Pb concentrations in its shoots. Compared with EDTA, EDDS was more effective in enhancing the concentration of Cu in the shoots of Chrysanthemum coronarium L. and Zea mays L. grown on multi-metal contaminated soils. The EDTA-treated soil still had a significant ability to enhance the concentrations of Cu and Pb in the shoots of Zea mays L. six months after the chelant treatment. However, the EDDS-treated soil did not have any effect in enhancing the concentrations of metals in the shoots of Zea mays L. in the second crop test. The results may indicate that EDDS biodegrades more rapidly than EDTA in soil and is better in limiting potential metal leaching. - Chrysanthemum coronarium L. was the most sensitive species to the application of chelants, and EDDS biodegrades much more rapidly than EDTA in soil

  11. Can Cd translocation in Oryza sativa L. be attenuated by arbuscular mycorrhizal fungi in the presence of EDTA?

    Science.gov (United States)

    Huang, Xiaochen; An, Guangnan; Zhu, Shishu; Wang, Li; Ma, Fang

    2018-04-01

    Arbuscular mycorrhizal (AM) fungi play an important role in plant tolerance of heavy metal contamination. In this study, a pot experiment was conducted to illustrate the effects of the two AM fungi species Funneliformis mosseae (Fm) and Rhizophagus irregularis (Ri) on plant growth of Oryza sativa L. either with or without ethylenediamine tetraacetate (EDTA) addition and during exposure to five Cd concentrations (in the range of 0-5 mg kg -1 ). The results showed that Fm inoculation achieved greater mycorrhizal colonization and mycorrhizal dependency indexes than Ri inoculation. In addition, the effects of AM fungi on Cd biosorption and translocation in rice were also investigated in the presence of EDTA. Despite cooperative adsorption, the Freundlich isotherm could describe the biosorption effects of Cd on rice roots regardless of AM fungi inoculation or EDTA addition. Cd concentrations in mycorrhizal roots increased but decreased in mycorrhizal shoots in contrast to the control treatment. Although EDTA addition negatively inhibited the uptake of Cd to mycorrhizal shoots, lower translocation factor (TF) and bioconcentration factor (BCF) were still observed in treatments with EDTA compared to control treatment. Our findings suggest that Ri and Fm inoculation enhanced Cd immobilization in the roots, thus preventing Cd entry into the food chain during exposure to low and high Cd stress, respectively.

  12. Intestinal permeability to (/sup 51/Cr)EDTA in children with Crohn's disease and celiac disease

    Energy Technology Data Exchange (ETDEWEB)

    Turck, D.; Ythier, H.; Maquet, E.; Deveaux, M.; Marchandise, X.; Farriaux, J.P.; Fontaine, G.

    1987-07-01

    (/sup 51/Cr)EDTA was used as a probe molecule to assess intestinal permeability in 7 healthy control adults, 11 control children, 17 children with Crohn's disease, and 6 children with untreated celiac disease. After subjects fasted overnight, 75 kBq/kg (= 2 microCi/kg) /sup 51/Cr-labeled EDTA was given by mouth; 24-h urinary excretion of (/sup 51/Cr)EDTA was measured and expressed as a percentage of the total oral dose. Mean and SD were as follows: control adults 1.47 +/- 0.62, control children 1.59 +/- 0.55, and patients with Crohn's disease or celiac disease 5.35 +/- 1.94. The difference between control children and patients was statistically significant (p less than 0.001). These results show that intestinal permeability to (/sup 51/Cr)EDTA is increased among children with active or inactive Crohn's disease affecting small bowel only or small bowel and colon, and with untreated celiac disease. The (/sup 51/Cr)EDTA permeability test could facilitate the decision to perform more extensive investigations in children suspected of small bowel disease who have atypical or poor clinical and biological symptomatology.

  13. Thermal decomposition of lanthanides (III) and yttrium (III) solid complexes from ethyl ene diamine tetraacetic acid

    International Nuclear Information System (INIS)

    Mercadante, A.

    1991-01-01

    Solid state compounds of lanthanides (III) and yttrium derived from ethyl ene diamine tetraacetic acid were prepared from respective basic carbonates, that were neutralized with EDTA stoichiometry quantities. Complexometry with EDTA, thermogravimetry (TG), differential thermal analysis (DTA) and X-ray diffraction have been used in the study of these compounds. The results of complexometry with EDTA as well as TG and DTA curves bed to the stoichiometry of these compounds the following general formula is obeyed: H[Ln(EDTA]. n H 2 O. X-ray powder patterns of these compounds permitted to establish two isomorphous series. The DTA ant TG curves allowed us to study the dehydration process, the thermal stability and thermal decomposition of these compounds. (C.G.C.)

  14. Enhanced electrokinetic remediation of lead-contaminated soil by complexing agents and approaching anodes.

    Science.gov (United States)

    Zhang, Tao; Zou, Hua; Ji, Minhui; Li, Xiaolin; Li, Liqiao; Tang, Tang

    2014-02-01

    Optimizing process parameters that affect the remediation time and power consumption can improve the treatment efficiency of the electrokinetic remediation as well as determine the cost of a remediation action. Lab-scale electrokinetic remediation of Pb-contaminated soils was investigated for the effect of complexant ethylenediaminetetraacetic acid (EDTA) and acetic acid and approaching anode on the removal efficiency of Pb. When EDTA was added to the catholyte, EDTA dissolved insoluble Pb in soils to form soluble Pb-EDTA complexes, increasing Pb mobility and accordingly removal efficiency. The removal efficiency was enhanced from 47.8 to 61.5 % when the EDTA concentration was increased from 0.1 to 0.2 M, showing that EDTA played an important role in remediation. And the migration rate of Pb was increased to 72.3 % when both EDTA and acetic acid were used in the catholyte. The "approaching anode electrokinetic remediation" process in the presence of both EDTA and acetic acid had a higher Pb-removal efficiency with an average efficiency of 83.8 %. The efficiency of electrokinetic remediation was closely related to Pb speciation. Exchangeable and carbonate-bounded Pb were likely the forms which could be removed. All results indicate that the approaching anode method in the presence of EDTA and acetic acid is an advisable choice for electrokinetic remediation of Pb-contaminated soil.

  15. Reversibility of increased intestinal permeability to 51Cr-EDTA in patients with gastrointestinal inflammatory diseases

    International Nuclear Information System (INIS)

    Jenkins, R.T.; Jones, D.B.; Goodacre, R.L.; Collins, S.M.; Coates, G.; Hunt, R.H.; Bienenstock, J.

    1987-01-01

    Intestinal permeability in adults with inflammatory gastrointestinal diseases was investigated by measuring the 24-h urinary excretion of orally administered 51 Cr-EDTA. Eighty controls along with 100 patients with Crohn's disease, 46 patients with ulcerative colitis, 20 patients with gluten-sensitive enteropathy, and 18 patients with other diseases were studied. In controls, the median 24-h excretion was 1.34%/24 h of the oral dose. Patients with Crohn's disease (median 2.96%/24 h), ulcerative colitis (median 2.12%/24 h), and untreated gluten-sensitive enteropathy (median 3.56%/24 h) had significantly elevated urinary excretion of the probe compared to controls (p less than 0.0001). Increased 24-h urinary excretion of 51 Cr-EDTA had a high association with intestinal inflammation (p less than 0.0001). Test specificity and sensitivity were 96% and 57%, respectively. A positive test has a 96% probability of correctly diagnosing the presence of intestinal inflammation, whereas a negative test has a 50% probability of predicting the absence of disease

  16. Development of Tubular Type Underwater Discharge Reactor to decompose Fe-EDTA from aqueous solution

    International Nuclear Information System (INIS)

    Kang, Duck-Won; Kim, Seok-Tae; Kim, Jin Kil; Ki, Hyung-Dong

    2007-01-01

    In case of a nuclear industry, the wastewater is hardly generated in normal operating conditions aside from laundry rooms, particularly for wastewater contaminated by radioactive materials. However if the steam generator (SG) chemical cleaning works are carrying out, it is another story. In this case we have to predict wastewater production at least from several tons to several hundreds tons during the works. Actually Kori Unit 4 in Korea is preparing the advanced sludge conditioning agents (ASCAs) project at the next overhaul period, June-2007, to remove the tube sheet scale, and we are predicting that the 200 . 250 tons waste solutions are going to produce during this works. SG chemical cleaning waste solution containing chelating agents such as EDTA is hardly easy to purify and radioactive materials included in this solution make much harder. Therefore we must have technologies to purify this chemical cleaning waste solution. The best wastewater treatment system should have great adaptability, low environmental impact, low amount of hazardous waste, and low capital and operating costs. In this study we developed the underwater spark discharge system (USDS) to decompose Fe-EDTA from aqueous solution which is contaminated with radioactive materials

  17. Effect of heavy metal and edta application on plant growth and phyto-extraction potential of sorghum (sorghum bicolor)

    International Nuclear Information System (INIS)

    Bacaha, N.; Shamas, R.; Bakht, J.; Rafi, A.; Farhatullah, M.; Gillani, S.

    2015-01-01

    Pot experiment was conducted to evaluate the phyto-extraction capacity of heavy metals by Sorghum. Sorghum bicolor was grown in soil artificially contaminated with different concentrations of lead (300, 350 and 400 mg/kg), chromium (50, 100 and 150 mg/kg) and cadmium (100, 150 and 200 mg/kg). Five mM EDTA was applied, as chelating agent to the plants after 4 weeks of sowing. Plants were grown for a total of two months and fresh weight and dry weight of shoot and heavy metal accumulation were analyzed at six and eight weeks after sowing. The results revealed that application of cadmium, chromium and lead and EDTA adversely affected shoot length, fresh weight and dry weight of S. bicolor at both time intervals. Heavy metals uptake increased with the increment of heavy metal by S. bicolor species. Application of 5mM EDTA enhanced the uptake of heavy metal. (author)

  18. Pre-storage centrifugation conditions have significant impact on measured microRNA levels in biobanked EDTA plasma samples

    DEFF Research Database (Denmark)

    Binderup, Helle Glud; Houlind, Kim; Madsen, Jonna Skov

    2016-01-01

    in platelets this may partly be explained by residual platelets in the plasma samples used. When collecting fresh plasma samples, it is possible to produce cell-free/platelet-poor plasma by centrifugation. In this study, we systematically investigated whether biobanked EDTA plasma samples could be processed...... to be suitable for miRNA analysis. Materials and methods: Blood samples were collected from ten healthy volunteers and centrifuged to produce platelet-poor-plasma (PPP) and standard biobank plasma. After one week at -80 °C the biobanked EDTA plasma was re-centrifuged by different steps to remove residual...... platelets. Using RT-qPCR the levels of 14 miRNAs in the different plasma preparations were compared to that of PPP. Results: We were able to remove residual platelets from biobanked EDTA plasma by re-centrifugation of the thawed samples. Nevertheless, for most of the investigated miRNAs, the miRNA level...

  19. Relationship between intestinal permeability to [51Cr]EDTA and inflammatory activity in asymptomatic patients with Crohn's disease

    International Nuclear Information System (INIS)

    Pironi, L.; Miglioli, M.; Ruggeri, E.; Levorato, M.; Dallasta, M.A.; Corbelli, C.; Nibali, M.G.; Barbara, L.

    1990-01-01

    The relationship between intestinal permeability to an oral dose (100 mu Ci) of [51CR]EDTA and the inflammatory activity of Crohn's disease was studied in 63 adult patients (32 unresected and 31 resected) who underwent 162 evaluations. The results of the [51CR]EDTA test were compared with the serum levels of the acute-phase reactant proteins (APRP) and with the result of the [111In]leukocyte scanning, respectively, as an indirect and direct method to assess intestinal inflammation. In a group of healthy adult controls, the upper normal value for the 24-hr urinary [51CR]EDTA excretion was 3.61 (97.5% percentile) and the mean coefficient of variation was 21%. Sensitivity and specificity of the [51CR]EDTA test in identifying active inflammation expressed by increased serum levels of APRP were, respectively, 97% and 54% in the unresected group and 68% and 52% in the resected group of patients. The low specificity of the test was due to the presence of increased [51CR]EDTA urinary excretion in about half the cases with normal serum levels of APRP. The [111In]leukocyte scanning was performed in a subgroup of 11 patients (three unresected and eight resected) with normal serum levels of APRP, six with increased and five with normal [51CR]EDTA urinary excretion. All six patients with increased intestinal permeability had a positive 111In image of mild to moderate degree of activity. A positive 111In scan was present in two of the five patients with normal permeability; these were two resected patients

  20. Microbial reduction of [Co(III)–EDTA]− by Bacillus licheniformis SPB-2 strain isolated from a solar salt pan

    International Nuclear Information System (INIS)

    Paraneeiswaran, Arunachalam; Shukla, Sudhir K.; Prashanth, K.; Rao, T. Subba

    2015-01-01

    Graphical abstract: - Highlights: • Bacillus licheniformis SPB-2 was used in the bioremediation of [Co(III)–EDTA] − . • The bacterial biomass adsorbed the Co–EDTA complex after its reduction. • [Co(III)–EDTA] − complex showed Bacillus spore inducing property. • B. licheniformis SPB-2 showed significantly radio-tolerance (D 10 = 250 Gy). - Abstract: Naturally stressed habitats are known to be repositories for novel microorganisms with potential bioremediation applications. In this study, we isolated a [Co(III)–EDTA] − reducing bacterium Bacillus licheniformis SPB-2 from a solar salt pan that is exposed to constant cycles of hydration and desiccation in nature. [Co(III)–EDTA] − generated during nuclear waste management process is difficult to remove from the waste due to its high stability and solubility. It is reduced form i.e. [Co(II)–EDTA] 2− is less stable though it is toxic. This study showed that B. licheniformis SPB-2 reduced 1 mM [Co(III)–EDTA] − in 14 days when grown in a batch mode. However, subsequent cycles showed an increase in the reduction activity, which was observed up to four cycles. Interestingly, the present study also showed that [Co(III)–EDTA] − acted as an inducer for B. licheniformis SPB-2 spore germination. Vegetative cells germinated from the spores were found to be involved in [Co(III)–EDTA] − reduction. More detailed investigations showed that after [Co(III)–EDTA] − reduction, i.e. [Co(II)–EDTA] 2− complex was removed by B. licheniformis SPB-2 from the bulk liquid by adsorption phenomenon. The bacterium showed a D 10 value (radiation dose required to kill 90% cells) of ∼250 Gray (Gy), which signifies the potential use of B. licheniformis SPB-2 for bioremediation of moderately active nuclear waste

  1. Microbial reduction of [Co(III)–EDTA]{sup −} by Bacillus licheniformis SPB-2 strain isolated from a solar salt pan

    Energy Technology Data Exchange (ETDEWEB)

    Paraneeiswaran, Arunachalam [Departartment of Biotechnology, Pondicherry University, Puducherry (India); Shukla, Sudhir K. [Biofouling and Biofilm Processes Section, Water and Steam Chemistry Division, BARC Facilities, Kalpakkam 603102 (India); Homi Bhabha National Institute, Mumbai 400094 (India); Prashanth, K. [Departartment of Biotechnology, Pondicherry University, Puducherry (India); Rao, T. Subba, E-mail: subbarao@igcar.gov.in [Biofouling and Biofilm Processes Section, Water and Steam Chemistry Division, BARC Facilities, Kalpakkam 603102 (India); Homi Bhabha National Institute, Mumbai 400094 (India)

    2015-02-11

    Graphical abstract: - Highlights: • Bacillus licheniformis SPB-2 was used in the bioremediation of [Co(III)–EDTA]{sup −}. • The bacterial biomass adsorbed the Co–EDTA complex after its reduction. • [Co(III)–EDTA]{sup −} complex showed Bacillus spore inducing property. • B. licheniformis SPB-2 showed significantly radio-tolerance (D{sub 10} = 250 Gy). - Abstract: Naturally stressed habitats are known to be repositories for novel microorganisms with potential bioremediation applications. In this study, we isolated a [Co(III)–EDTA]{sup −} reducing bacterium Bacillus licheniformis SPB-2 from a solar salt pan that is exposed to constant cycles of hydration and desiccation in nature. [Co(III)–EDTA]{sup −} generated during nuclear waste management process is difficult to remove from the waste due to its high stability and solubility. It is reduced form i.e. [Co(II)–EDTA]{sup 2−} is less stable though it is toxic. This study showed that B. licheniformis SPB-2 reduced 1 mM [Co(III)–EDTA]{sup −} in 14 days when grown in a batch mode. However, subsequent cycles showed an increase in the reduction activity, which was observed up to four cycles. Interestingly, the present study also showed that [Co(III)–EDTA]{sup −} acted as an inducer for B. licheniformis SPB-2 spore germination. Vegetative cells germinated from the spores were found to be involved in [Co(III)–EDTA]{sup −} reduction. More detailed investigations showed that after [Co(III)–EDTA]{sup −} reduction, i.e. [Co(II)–EDTA]{sup 2−} complex was removed by B. licheniformis SPB-2 from the bulk liquid by adsorption phenomenon. The bacterium showed a D{sub 10} value (radiation dose required to kill 90% cells) of ∼250 Gray (Gy), which signifies the potential use of B. licheniformis SPB-2 for bioremediation of moderately active nuclear waste.

  2. Titulações potenciométricas de cátions metálicos tendo como eletrodo indicador o sistema Cu/Cu(II-EDTA Potentiometric titrations of metal cations with edta using the Cu/Cu(II-EDTA system as indicator electrode

    Directory of Open Access Journals (Sweden)

    Paulo H. Pereira da Silva

    2008-01-01

    Full Text Available In potentiometric titrations of metal cations with EDTA the Hg/HgY2- system is usually used to detect the end point. However, the use of mercury has been discouraged in analytical procedures due to its toxicity. In this work the Cu/CuY2- system was used as indicator electrode for potentiometric titrations of some metal cations with EDTA. The solutions of Cu2+, Cd2+, Mn2+, Co2+ and Zn2+ were titrated with Na2EDTA solution in the presence of a small concentration of the CuY2- complex using a copper wire as indicator electrode. The potentiometric titrations with the Cu/CuY2- system showed good correlation when compared with an Hg/HgY2- system.

  3. The effectiveness of cooling conditions on temperature of canine EDTA whole blood samples.

    Science.gov (United States)

    Tobias, Karen M; Serrano, Leslie; Sun, Xiaocun; Flatland, Bente

    2016-01-01

    Preanalytic factors such as time and temperature can have significant effects on laboratory test results. For example, ammonium concentration will increase 31% in blood samples stored at room temperature for 30 min before centrifugation. To reduce preanalytic error, blood samples may be placed in precooled tubes and chilled on ice or in ice water baths; however, the effectiveness of these modalities in cooling blood samples has not been formally evaluated. The purpose of this study was to evaluate the effectiveness of various cooling modalities on reducing temperature of EDTA whole blood samples. Pooled samples of canine EDTA whole blood were divided into two aliquots. Saline was added to one aliquot to produce a packed cell volume (PCV) of 40% and to the second aliquot to produce a PCV of 20% (simulated anemia). Thirty samples from each aliquot were warmed to 37.7 °C and cooled in 2 ml allotments under one of three conditions: in ice, in ice after transfer to a precooled tube, or in an ice water bath. Temperature of each sample was recorded at one minute intervals for 15 min. Within treatment conditions, sample PCV had no significant effect on cooling. Cooling in ice water was significantly faster than cooling in ice only or transferring the sample to a precooled tube and cooling it on ice. Mean temperature of samples cooled in ice water was significantly lower at 15 min than mean temperatures of those cooled in ice, whether or not the tube was precooled. By 4 min, samples cooled in an ice water bath had reached mean temperatures less than 4 °C (refrigeration temperature), while samples cooled in other conditions remained above 4.0 °C for at least 11 min. For samples with a PCV of 40%, precooling the tube had no significant effect on rate of cooling on ice. For samples with a PCV of 20%, transfer to a precooled tube resulted in a significantly faster rate of cooling than direct placement of the warmed tube onto ice. Canine EDTA whole blood samples cool most

  4. Should patients with CKD stage 5D and biochemical evidence of secondary hyperparathyroidism be prescribed calcimimetic therapy? An ERA-EDTA position statement

    NARCIS (Netherlands)

    Goldsmith, David; Covic, Adrian; Vervloet, Marc; Cozzolino, Mario; Nistor, Ionut; Vervloet, Mark; Brandenburg, Vincent; Bover, Jordi; Evenepoel, Pieter; Massy, Ziad; Mazzaferro, Sandro; Urena-Torres, Pablo; Abramowicz, D.; Bolignano, D.; Cannata Andia, G.; Cochat, P.; Covic, A.; Delvecchio, L.; Drechsler, C.; Eckardt, K. U.; Fouque, D.; Fox, J.; Haller, M.; Heimburger, O.; Jager, K. J.; Lindley, E.; Marti Monros, A. M.; Nagler, E.; Oberbauer, R.; Spasovski, G.; Tattersall, J.; van Biesen, W.; Vander Veer, S.; Vanholder, R.; Wanner, C.; Wheeler, D.; Whithers, W.; Wiecek, A.; Zoccali, C.

    2015-01-01

    This paper reflects the position of the CKD-MBD workgroup, an official working group of ERA-EDTA and of the ERBP advisory board, the official guideline-producing body of ERA-EDTA, on the topic of the use of calcimimetics in patients with CKD stage 5D, as based on two recent meta-analysis

  5. Two-dimensional layer architecture assembled by Keggin polyoxotungstate, Cu(II)-EDTA complex and sodium linker: Synthesis, crystal structures, and magnetic properties

    International Nuclear Information System (INIS)

    Liu Hong; Xu Lin; Gao Guanggang; Li Fengyan; Yang Yanyan; Li Zhikui; Sun Yu

    2007-01-01

    Reaction of Keggin polyoxotungstate with copper(II)-EDTA (EDTA=ethylenediamine tetraacetate) complex under mild conditions led to the formation of hybrid inorganic-organic compounds Na 4 (OH)[(Cu 2 EDTA)PW 12 O 40 ].17H 2 O (1) and Na 4 [(Cu 2 EDTA)SiW 12 O 40 ].19H 2 O (2). The single-crystal X-ray diffraction analyses reveal their two structural features: (1) one-dimensional chain structure consisting of Keggin polyoxotungstate and copper(II)-EDTA complex; (2) Two-dimensional layer architecture assembled by the one-dimensional chain structure and sodium linker. The results of magnetic measurements in the temperature range 300-2 K indicated the existence of ferromagnetic exchange interactions between the Cu II ions for both compounds. In addition, TGA analysis, IR spectra, and electrochemical properties were also investigated to well characterize these two compounds. - Graphical abstract: Two new polyoxometalate-based hybrids, Na 4 (OH)[Cu 2 (EDTA)PW 12 O 40 ].17H 2 O (1) and Na 4 [Cu 2 (EDTA)SiW 12 O 40 ].19H 2 O (2), have been synthesized and structurally characterized, which consist of one-dimensional chain structure assembled by Keggin polyoxotungstate and copper(II)-EDTA complex. The chains are further connected to form two-dimensional layer architecture assembled by the one-dimensional chain structure and sodium linker

  6. EDTA-induced phytoextraction of lead and barium by brachiaria (B. decumbens cv. Basilisk in soil contaminated by oil exploration drilling waste

    Directory of Open Access Journals (Sweden)

    André Fernão Martins de Andrade

    2014-08-01

    Full Text Available The phytoextraction of heavy metals using chelating agents has been widely studied for the remediation of contaminated soils. To evaluate the efficiency of EDTA-induced phytoextraction of Ba and Pb using Brachiaria decumbens for the remediation of soil contaminated by oil well drilling and exploration waste, an experiment was conducted by applying a single dose (6 mmol EDTA kg-1 soil and split doses of EDTA (three applications of 2 mmol EDTA kg-1 soil. The samples were subjected to sequential extractions using the method proposed by Ure et al. (1993 as modified by Rauret et al. (1999.The application of EDTA did not influence the distribution of Ba in various chemical fractions of the soil. The dry matter production did not differ significantly between the treatments and the control, thereby demonstrating the tolerance of plants to the experimental conditions. The absorption of Pb by plants was influenced by the application of EDTA. The application of a single dose of EDTA influenced the absorption of Pb and its translocation to the aerial plant parts. The application of split doses favoured higher accumulation of Pb in roots. Because of its tolerance to heavy metals and EDTA, B. decumbens has the potential to be used in phytostabilisation.

  7. Blood cell attachment to root surfaces treated with EDTA gel Adesão de células sangüíneas a superfícies radiculares tratadas com gel de EDTA

    Directory of Open Access Journals (Sweden)

    Fábio Renato Manzolli Leite

    2005-06-01

    Full Text Available Root debridement generates a smear layer which contains microorganisms and toxins that could interfere in periodontal healing. For this reason, different substances have been used to remove it and to expose collagen fibers at the tooth surface. Blood element adhesion to demineralized roots and clot stabilization by collagen fibers are extremely important for the success of periodontal surgery. The aim of this study was to evaluate the different patterns of blood element adsorption and adhesion to root surfaces only irrigated with distilled water and after application of a manipulated or an industrialized EDTA gel. Thirty samples were planed, equally divided into three groups and treated with distilled water (control, a manipulated EDTA gel or an industrialized one. Immediately after, samples were exposed to fresh blood and prepared for scanning electron microscopy. Untreated planed dentin presented the best results with blood cells entrapped in a thick web of fibrin. In the manipulated EDTA group, the web of fibrin was thick with sparse blood elements. The worst result was seen with the industrialized EDTA group, in which no blood elements could be seen. Statistical difference was obtained between control and industrialized EDTA groups. Surfaces only irrigated presented the most organized fibrin network and cell entrapment.A raspagem gera "smear layer", a qual contém microrganismos e toxinas que podem interferir no reparo periodontal. Por esse motivo, diferentes substâncias têm sido empregadas para remover esta camada e expor fibras colágenas da superfície dental. A adesão de elementos sangüíneos a superfícies radiculares desmineralizadas e a estabilização do colágeno pelas fibras colágenas são de extrema importância no sucesso da cirurgia periodontal. O objetivo deste estudo foi avaliar os diferentes padrões de adsorção e adesão de elementos sangüíneos a superfícies radiculares apenas irrigadas com água destilada e ap

  8. Decline in 51Cr-labelled EDTA measured glomerular filtration rate following lung transplantation

    DEFF Research Database (Denmark)

    Hornum, Mads; Burton, Christopher M; Iversen, Martin

    2007-01-01

    -labelled EDTA clearance (mGFR) and the Cockcroft-Gault calculated clearance (cGFR). Trough cyclosporine levels (C0) and demographic and transplant information were also included in the analysis. RESULTS: A total of 66959 C0 and serum creatinine and 1945 mGFR measurements pertaining to 383 patients were included...... in the analysis. Pre-transplant mGFR was significantly lower with respect to recipient age over 60 years; and patients with a referral diagnosis of pulmonary hypertension had a lower mGFR and higher baseline serum creatinine levels than patients with emphysematous disease (P ... correlations between log(10) mean interval serum creatinine and log(2) mGFR at all time points pre- and post-transplantation (P

  9. Estimation of stability of polynuclear rare earth complexes with EDTA in the presence of stable monocomplexonates

    International Nuclear Information System (INIS)

    Le Ba Tkhun; Kupriyanova, G.N.; Smirnova, N.S.; Martynenko, L.I.; Evseeva, A.M.

    1987-01-01

    Complexing in rare-earth-EDTA system at the mole ratio making up (M 3+ ):(H 2 A 2- )=1:1, 2:1, 5:1, (M=Nd, Yb), C H 2 A 2- =0.010, 0.015 mol/l, μ=0.5 (KCl) is studied in pH 1.6-3.0 interval by pH-metric titration technique using mathematical modelling method. A model containing MA - , MHA, M 2 A 2+ complexes for which stability constants, equalling respectively (lgK) to 15.66, 2.11, 1.06 (for neodymium complexes) and 18.70, 1.78, 0.45 (for ytterbium complexes) are determined by non-linear LSF, appears to be adequate. It is shown that binuclear complex concentration in solution with M 3+ excess is comparable to stable monocomplexonate concentration

  10. Single injection 51Cr EDTA plasma clearance determination in children using capillary blood samples

    International Nuclear Information System (INIS)

    Broechner-Mortensen, J.; Christoffersen, J.

    1977-01-01

    The reliability of a determination of the total 51 Cr EDTA plasma clearance (e) (and with it the glomerular filtration rate), by a simplified single injection method (injected dose: 4.5 μCi per kg b.w.) using capillary blood samples (0.2 ml), was investigated in twenty children. Clearance values determined from capillary blood samples did not differ significantly from those measured simultaneously from venous blood samples, the mean ratio+-SD being 1.02+-0.06(n = 10). The reproducibility (total day-to-day variation) of E determined from capillary blood samples was 6.7% in children with decreased renal function (n = 3) and 6.9% in children with normal renal function (n = 7). The present data indicate that the use of capillary blood samples is an accurate and very precise approach for determination of E in children. (Auth.)

  11. Adsorption and performance of the 2-mercaptobenzimidazole as a carbon steel corrosion inhibitor in EDTA solutions

    International Nuclear Information System (INIS)

    Calderón, J.A.; Vásquez, F.A.; Carreño, J.A.

    2017-01-01

    This study presents a thermodynamic analysis of the adsorption and anti-corrosion performance of 2-mercaptobenzimidazole (2-MBI) on carbon steel in EDTA-Na2 solutions. The adsorption of the inhibitor on the metal surface was studied as a function of the concentration of the inhibiting species and the temperature of the system. The corrosion inhibition efficiency was studied by electrochemical impedance spectroscopy and mass loss tests. The results show that the adsorption of the inhibitor onto the metal surface behaves according to the Langmuir model, following an endothermic process. The inhibitor is chemically adsorbed onto the carbon steel surface. The efficiency of corrosion inhibition was above 93%, which was confirmed by both mass loss tests and the electrochemical impedance technique. The good performance of the corrosion inhibitor was maintained up to 24 h after the inhibitor was added to the corrosive EDTA-Na2 solutions. When the ratio of the volume of solution/exposed area was reduced, a decrease in the area covered by the inhibitor was observed. The best cost/benefit ratio for the corrosion protection of carbon steel was obtained when the number of moles of the inhibitor per surface area was maintained at 2.68 mmol cm"−"2. - Highlights: • Adsorption of the inhibitor on the metal surface is confirmed by thermodynamic data. • Adsorption of the inhibitor onto the metal behaves according to the Langmuir model. • Endothermic adsorption process indicates that the inhibitor is chemically adsorbed. • The efficiency of corrosion inhibition was above 93%. • The good performance of the corrosion inhibitor was maintained up to 24 h.

  12. Adsorption and performance of the 2-mercaptobenzimidazole as a carbon steel corrosion inhibitor in EDTA solutions

    Energy Technology Data Exchange (ETDEWEB)

    Calderón, J.A., E-mail: andres.calderon@udea.edu.co [Centro de Investigación, Innovación y Desarrollo de Materiales –CIDEMAT, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín (Colombia); Vásquez, F.A. [Centro de Investigación, Innovación y Desarrollo de Materiales –CIDEMAT, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín (Colombia); Carreño, J.A. [Laboratório de H2S, CO2 e Corrosividade, Instituto Nacional De Tecnologia (INT), Av. Venezuela, 82 – Térreo, Anexo 01, Sala 101A, Saúde, Rio de Janeiro, RJ (Brazil)

    2017-01-01

    This study presents a thermodynamic analysis of the adsorption and anti-corrosion performance of 2-mercaptobenzimidazole (2-MBI) on carbon steel in EDTA-Na2 solutions. The adsorption of the inhibitor on the metal surface was studied as a function of the concentration of the inhibiting species and the temperature of the system. The corrosion inhibition efficiency was studied by electrochemical impedance spectroscopy and mass loss tests. The results show that the adsorption of the inhibitor onto the metal surface behaves according to the Langmuir model, following an endothermic process. The inhibitor is chemically adsorbed onto the carbon steel surface. The efficiency of corrosion inhibition was above 93%, which was confirmed by both mass loss tests and the electrochemical impedance technique. The good performance of the corrosion inhibitor was maintained up to 24 h after the inhibitor was added to the corrosive EDTA-Na2 solutions. When the ratio of the volume of solution/exposed area was reduced, a decrease in the area covered by the inhibitor was observed. The best cost/benefit ratio for the corrosion protection of carbon steel was obtained when the number of moles of the inhibitor per surface area was maintained at 2.68 mmol cm{sup −2}. - Highlights: • Adsorption of the inhibitor on the metal surface is confirmed by thermodynamic data. • Adsorption of the inhibitor onto the metal behaves according to the Langmuir model. • Endothermic adsorption process indicates that the inhibitor is chemically adsorbed. • The efficiency of corrosion inhibition was above 93%. • The good performance of the corrosion inhibitor was maintained up to 24 h.

  13. Effectiveness of EDTA as the irrigation solution to remove smear layer in root canal

    Directory of Open Access Journals (Sweden)

    Kurniasri Amas Achiar

    2009-07-01

    Full Text Available One of the objectives of successful endodontic treatment is the hermetic obturation of the root canal system. To achieve this, the root canal filling must seal the canal space both apically and coronally to prevent the ingress of microorganisms or tissue fluids into the canal space. Apical leakage is reported a common reason for the clinical failure of endodontic therapy. Leakage through an obturated root canal is expected to take place at interfaces between sealer and dentin or sealer and gutta-percha, or through voids within the sealer. Hence, the sealing quality of root canal filling depends much on the sealing ability of the sealer. Therefore, anything that may influence the adaptation of the root filling to the canal wall is can determine the degree and the extent of leakage, and ultimately the prognosis of the endodontic therapy. In endodontic therapy, the smear layer formation results from root canal preparation and may influence the effective seal of the root canal system. The smear layer formation is mainly composed of inorganic components (dentin debris and organic materials, such as pulp tissue remnant, bacteria, and blood cells. Removal of the smear layer from the root canal walls before the obturation can reduce the leakage of root canal sealer. To remove the smear layer use 10 ml 17% EDTA followed by 10 ml of 5.25% NaOCl as irrigating solution. This paper discribe about how the effectivity of EDTA as irigating solution to remove the smear layer especially in the apical root canal with many lateral canal to reduce the apical leakage.

  14. Characterization of Co(III) EDTA-Reducing Bacteria in Metal- and Radionuclide-Contaminated Groundwater

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Weimin [Arizona State University; Gentry, Terry J [ORNL; Mehlhorn, Tonia L [ORNL; Carroll, Sue L [ORNL; Jardine, Philip M [ORNL; Zhou, Jizhong [University of Oklahoma, Norman

    2010-01-01

    The Waste Area Grouping 5 (WAG5) site at Oak Ridge National Laboratory has a potential to be a field site for evaluating the effectiveness of various bioremediation approaches and strategies. The site has been well studied in terms of its geological and geochemical properties over the past decade. However, despite the importance of microorganisms in bioremediation processes, the microbiological populations at the WAG5 site and their potential in bioremediation have not been similarly evaluated. In this study, we initiated research to characterize the microbial populations in WAG5 groundwater. Approximately 100 isolates from WAG5 groundwater were isolated and selected based on colony morphology. Fifty-five unique isolates were identified by BOX-PCR and subjected to further characterization. 16S rRNA sequences indicated that these isolates belong to seventeen bacterial genera including Alcaligenes (1 isolate), Aquamonas (1), Aquaspirillum (1), Bacillus (10), Brevundimonas (5), Caulobacter (7), Dechloromonas (2), Janibacter (1), Janthinobacterium (2), Lactobacillus (1), Paenibacillus (4), Pseudomonas (9), Rhodoferax (1), Sphingomonas (1), Stenotrophomonas (6), Variovorax (2), and Zoogloea (1). Metal respiration assays identified several isolates, which phylogenically belong or are close to Caulobacter, Stenotrophomonas, Bacillus, Paenibacillus and Pseudomonas, capable of reducing Co(III)EDTA- to Co(II)EDTA{sup 2-} using the defined M1 medium under anaerobic conditions. In addition, using WAG5 groundwater directly as the inoculants, we found that organisms associated with WAG5 groundwater can reduce both Fe(III) and Co(III) under anaerobic conditions. Further assays were then performed to determine the optimal conditions for Co(III) reduction. These assays indicated that addition of various electron donors including ethanol, lactate, methanol, pyruvate, and acetate resulted in metal reduction. These experiments will provide useful background information for future

  15. Effects of liquid- and paste-type EDTA on smear-layer removal during rotary root-canal instrumentation

    Directory of Open Access Journals (Sweden)

    Gin Chen

    2011-03-01

    Conclusions: The use of paste/gel-type chelators during rotary nickel–titanium instrumentation resulted in improved cleanliness in the coronal and middle parts of the root canal. We recommend using liquid EDTA as a final flushing solution during root-canal preparation because it provides a better smear layer-free condition before 3-dimensional root-canal obturation.

  16. Het EDTA-gehalte van 20 monsters gesteriliseerde peulvruchten, in september 1984 gekocht te Karlsruhe en Emmerich

    NARCIS (Netherlands)

    Westerling, F.J.

    1984-01-01

    In tegenstelling tot Nederland is in Duitsland de toevoeging van EDTA (ethyleendiaminetetra-azijnzuur) aan geconserveerde peulvruchten niet toegestaan. Bij export van deze produkten naar Duitsland dient de nederlandse producent hier rekening mee te houden. In verband hiermee is een onderzoek

  17. Adsorption of hydroxamate siderophores and EDTA on goethite in the presence of the surfactant sodium dodecyl sulfate

    Directory of Open Access Journals (Sweden)

    Xu Jide

    2009-06-01

    Full Text Available Abstract Siderophore-promoted iron acquisition by microorganisms usually occurs in the presence of other organic molecules, including biosurfactants. We have investigated the influence of the anionic surfactant sodium dodecyl sulfate (SDS on the adsorption of the siderophores DFOB (cationic and DFOD (neutral and the ligand EDTA (anionic onto goethite (α-FeOOH at pH 6. We also studied the adsorption of the corresponding 1:1 Fe(III-ligand complexes, which are products of the dissolution process. Adsorption of the two free siderophores increased in a similar fashion with increasing SDS concentration, despite their difference in molecule charge. In contrast, SDS had little effect on the adsorption of EDTA. Adsorption of the Fe-DFOB and Fe-DFOD complexes also increased with increasing SDS concentrations, while adsorption of Fe-EDTA decreased. Our results suggest that hydrophobic interactions between adsorbed surfactants and siderophores are more important than electrostatic interactions. However, for strongly hydrophilic molecules, such as EDTA and its iron complex, the influence of SDS on their adsorption seems to depend on their tendency to form inner-sphere or outer-sphere surface complexes. Our results demonstrate that surfactants have a strong influence on the adsorption of siderophores to Fe oxides, which has important implications for siderophore-promoted dissolution of iron oxides and biological iron acquisition.

  18. Outcomes of renal replacement therapy in boys with prune belly syndrome: findings from the ESPN/ERA-EDTA Registry

    NARCIS (Netherlands)

    Yalcinkaya, Fatos; Bonthuis, Marjolein; Erdogan, Beyza Doganay; van Stralen, Karlijn J.; Baiko, Sergey; Chehade, Hassib; Maxwell, Heather; Montini, Giovanni; Rönnholm, Kai; Sørensen, Søren Schwartz; Ulinski, Tim; Verrina, Enrico; Weber, Stefanie; Harambat, Jérôme; Schaefer, Franz; Jager, Kitty J.; Groothoff, Jaap W.

    2018-01-01

    As outcome data for prune belly syndrome (PBS) complicated by end-stage renal disease are scarce, we analyzed characteristics and outcomes of children with PBS using the European Society for Pediatric Nephrology/European Renal Association-European Dialysis and Transplant Association (ESPN/ERA-EDTA)

  19. Disposition, accumulation and toxicity of iron fed as iron (II) sulfate or as sodium iron EDTA in rats

    NARCIS (Netherlands)

    Appel, M.J.; Kuper, C.F.; Woutersen, R.A.

    2001-01-01

    A study was performed to provide data on the disposition, accumulation and toxicity of sodium iron EDTA in comparison with iron (II) sulfate in rats on administration via the diet for 31 and 61 days. Clinical signs, body weights, food consumption, food conversion efficiency, hematology, clinical

  20. Influence of complexing agent (Na2EDTA on chemical bath deposited Cu4SnS4 thin films

    Directory of Open Access Journals (Sweden)

    Anuar Kassim

    2010-08-01

    Full Text Available The quality of thin film is influenced by the presence of complexing agents such as Na2EDTA. The Cu4SnS4 thin films were deposited onto indium tin oxide glass substrate by chemical bath deposition method. The structural, morphological and optical properties of the deposited films have been studied using X-ray diffraction, atomic force microscopy and UV-Vis spectrophotometer, respectively. The XRD data showed that the films have a polycrystalline and orthorhombic structure. It also indicated that the most intense peak at 2 θ = 30.2° which belongs to (221 plane of Cu4 SnS4. The film deposited with 0.05 M Na2 EDTA showed good uniformity, good surface coverage with bigger grains and produced higher absorbance value. The band gap energy varies with the variation of Na2EDTA concentration which ranging from 1.56-1.60 eV. Deposition at concentration of 0.05 M Na2EDTA proved to offer a reasonably good Cu4SnS4 thin film.

  1. Differences in EDTA-assisted metal phytoextraction between metallicolous and non-metallicolous accessions of Rumex acetosa L

    Energy Technology Data Exchange (ETDEWEB)

    Barrutia, Oihana, E-mail: oihana.barrutia@ehu.e [Department of Plant Biology and Ecology, University of the Basque Country/EHU, P. O. Box 644, E-48080 Bilbao (Spain); Garbisu, Carlos; Hernandez-Allica, Javier [NEIKER-Tecnalia, Basque Institute of Agricultural Research and Development, c/ Berreaga 1, E-48160 Derio (Spain); Garcia-Plazaola, Jose Ignacio; Becerril, Jose Maria [Department of Plant Biology and Ecology, University of the Basque Country/EHU, P. O. Box 644, E-48080 Bilbao (Spain)

    2010-05-15

    Two common sorrel (Rumex acetosa) accessions, one from a Zn-Pb contaminated site (CS accession) and the other from an uncontaminated site (UCS accession), were hydroponically exposed to a mixture of heavy metals (Pb{sup 2+} + Zn{sup 2+} + Cd{sup 2+}) with and without EDTA at an equimolar rate. The metallicolous CS accession showed a higher tolerance to metal treatment in the absence of the chelating agent, whereas the UCS accession was especially tolerant to EDTA treatment alone. Combination of metal and EDTA treatment resulted in a higher Pb accumulation in shoots of both accessions although plants hardly showed phytotoxic symptoms. Cd and Zn uptake was not augmented by EDTA addition to the polymetallic medium. Chelant-assisted Pb accumulation was 70% higher in the CS accession than in the UCS accession, despite the fact that the former accession evapotranspired less water than the UCS accession. These results support the existence of a non-selective apoplastic transport of metal chelates by R. acetosa roots, not related to transpiration stream. - A partial intolerance of plants to a certain chelating agent can contribute to the understanding of the mechanisms and pathways of metal chelates uptake.

  2. Differences in EDTA-assisted metal phytoextraction between metallicolous and non-metallicolous accessions of Rumex acetosa L

    International Nuclear Information System (INIS)

    Barrutia, Oihana; Garbisu, Carlos; Hernandez-Allica, Javier; Garcia-Plazaola, Jose Ignacio; Becerril, Jose Maria

    2010-01-01

    Two common sorrel (Rumex acetosa) accessions, one from a Zn-Pb contaminated site (CS accession) and the other from an uncontaminated site (UCS accession), were hydroponically exposed to a mixture of heavy metals (Pb 2+ + Zn 2+ + Cd 2+ ) with and without EDTA at an equimolar rate. The metallicolous CS accession showed a higher tolerance to metal treatment in the absence of the chelating agent, whereas the UCS accession was especially tolerant to EDTA treatment alone. Combination of metal and EDTA treatment resulted in a higher Pb accumulation in shoots of both accessions although plants hardly showed phytotoxic symptoms. Cd and Zn uptake was not augmented by EDTA addition to the polymetallic medium. Chelant-assisted Pb accumulation was 70% higher in the CS accession than in the UCS accession, despite the fact that the former accession evapotranspired less water than the UCS accession. These results support the existence of a non-selective apoplastic transport of metal chelates by R. acetosa roots, not related to transpiration stream. - A partial intolerance of plants to a certain chelating agent can contribute to the understanding of the mechanisms and pathways of metal chelates uptake.

  3. Influence of ionic strength and OH(-) ion concentration on the Cu(II) complex formation with EDTA in alkaline solutions.

    Science.gov (United States)

    Norkus, E; Vaskelis, A; Zakaite, I

    1996-03-01

    D.c. polarographic data show that the complex formation of copper ions with EDTA depends markedly on the ionic strength of the solution at pH 8-10. This is primarily associated with the dependence of the fourth deprotonization constant of EDTA on the solution ionic strength: when it increases from 0.4 to 3.4, the pK(a4) value decreases from 9.5 to 8.2. According to polarographic and spectrophotometric data the degree of Cu(II) complexation increases at pH>10 due to transformation of the complex CuY(2-) to the more stable CuY(OH)(3-) (Y(4-), a fully deprotonized anion of EDTA), but it decreases with increase in alkalinity in a highly alkaline solution (pH>13.5). The latter result could be explained by the decrease in the EDTA anion activity. The calculated values of the activity coefficient are lower than 0.05 at pH>14.

  4. Soft plasma electrolysis with complex ions for optimizing electrochemical performance

    Science.gov (United States)

    Kamil, Muhammad Prisla; Kaseem, Mosab; Ko, Young Gun

    2017-03-01

    Plasma electrolytic oxidation (PEO) was a promising surface treatment for light metals to tailor an oxide layer with excellent properties. However, porous coating structure was generally exhibited due to excessive plasma discharges, restraining its performance. The present work utilized ethylenediaminetetraacetic acid (EDTA) and Cu-EDTA complexing agents as electrolyte additives that alter the plasma discharges to improve the electrochemical properties of Al-1.1Mg alloy coated by PEO. To achieve this purpose, PEO coatings were fabricated under an alternating current in silicate electrolytes containing EDTA and Cu-EDTA. EDTA complexes were found to modify the plasma discharging behaviour during PEO that led to a lower porosity than that without additives. This was attributed to a more homogeneous electrical field throughout the PEO process while the coating growth would be maintai