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Sample records for estevia stevia rebaudiana

  1. SINTESIS DE ESTEVIOSIDOS EN ESTEVIA (Stevia rebaudiana Bert. STEVIOSIDE SYNTHESIS IN STEVIA (Stevia rebaudiana Bert

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    Alfredo Jarma Orozco

    Full Text Available Stevia rebaudiana Bert. es una planta selvática subtropical nativa de Paraguay, que posee un potente edulcorante de hasta 300 veces más dulce que la sacarosa y no tiene calorías. Las moléculas responsables de esta característica son glucósidos de diterpeno, presentes en hojas y sintetizados, al menos en los estados iniciales, usando la misma ruta de síntesis del ácido giberélico, en la que el ácido shiquímico, da origen a muchos compuestos aromáticos. El acetato es el precursor de los terpenos o isoprenoides por la ruta del acetato-mevalonato, donde se encuentran los esteviósidos, aunque en este artículo se discute una ruta alternativa. La presente actualización pretende aportar algunas herramientas para el entendimiento de las principales rutas de síntesis de los glucósidos de esteviol.Stevia rebaudiana Bert. is a subtropical wild plant of Paraguay , that possesses a potent sweetener up to 300 times higher than sucrose and has no calories. The molecules responsible for these characteristics are diterpen glycosides, found in leaves and synthesized at least, at initial states, which use the same pathway of the gibberellic acid in which the shiquimic acid gives origin to many aromatic compounds. The acetate is the precursor of the terpens or isoprenoids through the acetate-mevalonate pathway, where steviosides are found. Although in this article, an alternative route is discussed. This updating pretends to contribute tools for the understanding of the main pathways of steviol glycosides synthesis.

  2. SÍNTESIS DE ESTEVIOSIDOS EN ESTEVIA (Stevia rebaudiana BERT.

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    ALFREDO JARMA OROZCO

    2010-01-01

    Full Text Available Stevia rebaudiana Bert. es una planta selvática subtropical nativa de Paraguay, que posee un potente edulcorante de hasta 300 veces más dulce que la sacarosa y no tiene calorías. Las moléculas responsables de esta característica son glucósidos de diterpeno, presentes en hojas y sintetizados, al menos en los estados iniciales, usando la misma ruta de síntesis del ácido giberélico, en la que el ácido shiquímico, da origen a muchos compuestos aromáticos. El acetato es el precursor de los terpenos o isoprenoides por la ruta del acetato-mevalonato, donde se encuentran los esteviósidos, aunque en esta nota se discute una ruta alternativa. La presente actualización pretende aportar algunas herramientas para el entendimiento de las principales rutas de síntesis de los glucósidos de esteviol.

  3. Soil response to biodynamic farming practices in estevia -Stevia Rebaudiana- (Extremadura, Spain)

    Science.gov (United States)

    Labrador, Juana; Colmenares, Ricardo; Sánchez, Eduardo; Creus, Juan; García, Nieves; Blázquez, Jaime; Moreno, Marta M.

    2014-05-01

    The first results of the evolution of an organic-biodynamic cultivation of stevia (Stevia rebaudiana) in Extremadura (Spain) are shown here. The organic-biodynamic approach permits experimentally for a more holistic view of the crop development process what means the understanding and quantification of its evolution at different scales. The research methodology applied includes not only quantitative individual parameters of the crop development but also global parameters which make a contribution of very relevant information concerning unbalances between growth and differentiation processes, as well as other aspects linked to the product intrinsic quality. The crop cultivation has been done over a plot of 2.5 has, on acid soils (pH 5.18) and very poor organic matter content (0.5 %). On this first year of cultivation two cuts were given to the plant with an average total yield of 4,500 kg/ha without any supply of solid organic matter, only with the application of the biodynamic preparations. So far results regarding soil improvement and crop productivity, taking into consideration the practices used, let us introduce this pioneer crop in Extremadura, not only as an alternative crop to the current tobacco crop in this area, but also as a development resource for the rural environment of this region. Key words: Agroecology, Organic Biodynamic Agriculture, Stevia Rebaudiana

  4. Water consumption of the estevia (Stevia rebaudiana (Bert. Bertoni crop estimated through microlysimeter

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    Fronza Diniz

    2003-01-01

    Full Text Available The knowledge of water requirement of crops in the different growing phases elicits higher crop yield and rational use of water resource. The aim of this work was to estimate the water consumption of stevia using two constant watertable microlysimeters. The research was conducted in San Piero a Grado, Pisa, Italy. The data were collected daily from June, 1st, to October, 22th, 2000. Reference evapotranspiration was determined by the Penman-Monteith-FAO method, in the same period. Microlysimeters watertables level were maintained at the 35 cm depth. Crop evapotranspiration for the total cicle (80 days was 464 mm. For the most water consuming phase, crop average evapotranspiration was 5.44 mm day-1. The crop coefficient values were 1.45 for the first 25 days, 1.14 for the next period (26 to 50 days, and 1.16 for the latest period (51 to 80 days. The stevia leaf yield of the microlysimeters was 4.369 kg ha-1 and their steviosideo content 6.49%.

  5. Estabilidad fenotípica y densidades de planta de genotipos de estevia (Stevia rebaudiana Bert. en la región Caribe de Colombia

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    Jarma Orozco Alfredo

    2011-06-01

    Full Text Available Los estudios de estabilidad fenotípica son importantes para la siembra de genotipos que muestranun adecuado comportamiento en una o varias zonas. Estevia (Stevia rebaudiana Bert. es una especierecientemente introducida en Colombia, por tanto, los estudios sobre su adaptación en áreas productorasdel país son escasos. Esta planta se caracteriza por sintetizar glucósidos de diterpeno altamenteedulcorantes con bajo aporte calórico. El presente estudio se realizó en Montería (8º 52' N y 76º 48'O, Colombia, con el objeto de determinar la estabilidad fenotípica de los genotipos Morita 1 y Morita 2de estevia, sembrados en tres densidades de plantas (25, 12.5 y 6.25 plantas/m2 y dispuestos en undiseño de bloques completos al azar, en tres épocas de siembra en el Caribe húmedo de Colombia. Losresultados más importantes señalan que Morita 2, en densidades de 25 plantas/m2, registró los mejoresíndices de estabilidad para el rendimiento de hoja seca/ha (Pig = 0; Pid = 0. Por su parte, éste mismogenotipo presentó una mayor relación hoja/tallo (1.17 ± 0.3 y mayor contenido de glucósidos totales(0.1586 ± 0.024 cuando la radiación solar fue mayor y las densidades eran entre 12.5 y 25 plantas/m2.

  6. Estabilidad fenotípica y densidades de planta de genotipos de estevia (Stevia rebaudiana Bert. en la región Caribe de Colombia

    Directory of Open Access Journals (Sweden)

    Alfredo Jarma Orozco

    2011-04-01

    Full Text Available Los estudios de estabilidad fenotípica son importantes para la siembra de genotipos que muestran un adecuado comportamiento en una o varias zonas. Estevia (Stevia rebaudiana Bert. es una especie recientemente introducida en Colombia, por tanto, los estudios sobre su adaptación en áreas productoras del país son escasos. Esta planta se caracteriza por sintetizar glucósidos de diterpeno altamente edulcorantes con bajo aporte calórico. El presente estudio se realizó en Montería (8° 52' N y 76° 48' O, Colombia, con el objeto de determinar la estabilidad fenotípica de los genotipos Morita 1 y Morita 2 de estevia, sembrados en tres densidades de plantas (25, 12.5 y 6.25 plantas/m² y dispuestos en un diseño de bloques completos al azar, en tres épocas de siembra en el Caribe húmedo de Colombia. Los resultados más importantes señalan que Morita 2, en densidades de 25 plantas/m², registró los mejores índices de estabilidad para el rendimiento de hoja seca/ha (Pig = 0; Pid = 0. Por su parte, éste mismo genotipo presentó una mayor relación hoja/tallo (1.17 ± 0.3 y mayor contenido de glucósidos totales (0.1586 ± 0.024 cuando la radiación solar fue mayor y las densidades eran entre 12.5 y 25 plantas/m².

  7. Mapeo y análisis preliminar de la cadena de valor de la estevia (stevia rebaudiana bert.) en Colombia

    OpenAIRE

    Trujillo-Motta, Diego Mauricio

    2011-01-01

    La estevia es un producto nutracéutico o funcional, ya que de sus hojas se pueden extraer compuestos edulcorantes acalóricos (Esteviósidos y Rebaudiósidos) utilizados en la industria alimenticia en reemplazo del azúcar y de edulcorantes sintéticos para la elaboración de productos bajos en calorías. Adicionalmente, la estevia tiene propiedades terapéuticas para el tratamiento de diversas enfermedades. En la actualidad hay muy poca información disponible sobre la cadena productiva de la estevia...

  8. DETERMINATION DE GLÍCOSIDOS, EN MIEL EXPRESS DE ESTEVIA (Stevia rebaudianaPOR CROMATOGRAFÍA LÍQUIDA

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    LUÍS ALBERTO LENIS V.

    Full Text Available El objetivo del presente trabajo fue incorporar de forma natural en la miel los principales glicósido diterpenos dulces de la Stevia rebaudiana, denominados esteviósido y rebaudiósido A, con el fin de reunir las propiedades edulcorantes de estas dos sustancias; para comprobar su grado de incorporación se identificaron y cuantificaron los glicósido diterpenos tanto en los jarabes preparados como en la miel obtenida mediante la técnica analítica de cromatografía líquida de alta resolución (HPLC usando una columna NH2 (Zorbax-Agilent y como fase móvil una mezcla de acetonitrilo:agua (70:30 con detección en el rango ultravioleta a 194 nm. La cuantificación fue realizada por medio de una curva de calibración encontrándoseporcentajes de incorporación altos, superando el 97 % para esteviósido y rebaudiósido A; para la estandarización de la técnica analítica se determinaron parámetros como: linealidad, sensibilidad analítica, límite de detección, límite de cuantificación y precisión, demostrándose que el método desarrollado es simple, rápido y confiable, dentro de los límites establecidos, se detectaron niveles hasta de 0,08 ppm para steviósdio y 0,09 ppm para rebaudiósido A.

  9. Estabilidad fenotípica y densidades de planta de genotipos de estevia (Stevia rebaudiana Bert. en la región Caribe de Colombia Phenotypic stability and plant densities of stevia (Stevia rebaudiana Bert. genotypes in the Caribbean Region of Colombia

    Directory of Open Access Journals (Sweden)

    Alfredo Jarma Orozco

    2011-04-01

    Full Text Available Los estudios de estabilidad fenotípica son importantes para la siembra de genotipos que muestran un adecuado comportamiento en una o varias zonas. Estevia (Stevia rebaudiana Bert. es una especie recientemente introducida en Colombia, por tanto, los estudios sobre su adaptación en áreas productoras del país son escasos. Esta planta se caracteriza por sintetizar glucósidos de diterpeno altamente edulcorantes con bajo aporte calórico. El presente estudio se realizó en Montería (8° 52' N y 76° 48' O, Colombia, con el objeto de determinar la estabilidad fenotípica de los genotipos Morita 1 y Morita 2 de estevia, sembrados en tres densidades de plantas (25, 12.5 y 6.25 plantas/m² y dispuestos en un diseño de bloques completos al azar, en tres épocas de siembra en el Caribe húmedo de Colombia. Los resultados más importantes señalan que Morita 2, en densidades de 25 plantas/m², registró los mejores índices de estabilidad para el rendimiento de hoja seca/ha (Pig = 0; Pid = 0. Por su parte, éste mismo genotipo presentó una mayor relación hoja/tallo (1.17 ± 0.3 y mayor contenido de glucósidos totales (0.1586 ± 0.024 cuando la radiación solar fue mayor y las densidades eran entre 12.5 y 25 plantas/m².Phenotypic stability studies are important to recommend objectively the planting of genotypes showing appropriate behavior in one or more areas. Stevia (Stevia rebaudiana Bert., a species of recent cropping in Colombia, is characterized by the capacity of synthesize highly sweeteners diterpene glycosides with low calorie. However, while defining the environmental conditions for production, leaf /stem ratio in the canopy, and the amount of total glycosides, few studies of adaptation have been made in the country. This study was carried out in Montería-Colombia (8°52' N and 76°48' W to determine the phenotypic stability of two genotypes of stevia (Morita 1 and Morita 2 and three plant densities (25; 12.5 and 6.25 plants/ m² during

  10. Aspectos fisiológicos de estevia (Stevia rebaudiana Bertoni en el Caribe colombiano: I. Efecto de la radiación incidente sobre el área foliar y la distribución de biomasa

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    Jarma Alfredo

    2005-12-01

    Full Text Available

    Stevia rebaudiana Bertoni es uno de los 154 miembros del genero Stevia. El componente edulcorante de sus hojas se debe a glucosidos de diterpeno. Los principales glucosidos de esteviol son: esteviosido, rebaudiosido A, rebaudiosido C y dulcosido A. Este trabajo se realizo en Monteria (Colombia, con el proposito de evaluar el efecto de cuatro niveles de radiacion incidente sobre el comportamiento fisiologico de S. rebaudiana, bajo las condiciones ambientales delvalle del Sinu, en el Caribe colombiano. Se planteo un diseno completo al azar con arreglo factorial, en el que los factores fueron los porcentajes de la radiacion incidente en la region (19%, 24%, 56% y 100% y los genotipos de estevia ‘Morita 1’ y ‘Morita 2’. Los resultados indicaron que el area foliar de ‘Morita 2’ es superior a ‘Morita 1’, independientemente de la radiacion. Los altos niveles de radiacion incidente (100% y 56% registraron la mayor acumulacion de biomasa de las hojas, siendo superior en ‘Morita 2’. La mayor proporcion de la biomasa de las hojas, con respecto a la del tallo, en los primeros 60 d indica que la planta se dedico a fortalecer su aparato fotosintetico; luego, la migracion de fotoasimilados se hizo en mayor proporcion hacia los tallos, terminando con una tendencia estable hacia ambas demandas.

  11. Obtención de un filtrante de anís de monte (Tagetes filifolia Lag.) edulcorado con hojas de estevia (Stevia rebaudiana Bertoni)

    OpenAIRE

    Carlos Millones; Gerardo Mori; Joel Bacalla; Ernestina Vásquez; Rocío Tafur

    2014-01-01

    El presente trabajo de investigación se realizó para determinar el segmento de la planta de anís de monte yformulación apropiada de anís de monte y hojas de estevia en la elaboración de un filtrante edulcorado; para locual se emplearon plantas de anís demonte y hojas de estevia, las cuales fueron lavadas, desinfectadas,secadas a 65°C, molidas, tamizadas y envasadas; posteriormente se realizaron las formulaciones del filtrantecon anís de monte y hojas de estevia (90:10; 85:15; 80:20 y 75:25), ...

  12. Obtención de un filtrante de anís de monte (Tagetes filifolia Lag. edulcorado con hojas de estevia (Stevia rebaudiana Bertoni

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    Carlos Millones

    2014-01-01

    Full Text Available El presente trabajo de investigación se realizó para determinar el segmento de la planta de anís de monte yformulación apropiada de anís de monte y hojas de estevia en la elaboración de un filtrante edulcorado; para locual se emplearon plantas de anís demonte y hojas de estevia, las cuales fueron lavadas, desinfectadas,secadas a 65°C, molidas, tamizadas y envasadas; posteriormente se realizaron las formulaciones del filtrantecon anís de monte y hojas de estevia (90:10; 85:15; 80:20 y 75:25, empleandoun gramo por muestraenvasadas en papel termosellable; los resultados del color, tiempo de infusión y pH; asimismo, lasevaluaciones organolépticas de olor, sabor, dulzor y aspecto general se procesaron empleando el paqueteestadístico SAS (Statistical Analysis System for Window V8. Los resultados mostraron que empleando 80 a85% hojas + flores de anís de monte y 15 a 20% de hojas de estevia se obtiene un filtrante edulcorado conadecuadas características organolépticas.

  13. Cultivation and uses of stevia ( Stevia rebaudiana Bertoni): a review ...

    African Journals Online (AJOL)

    Stevia [Stevia rebaudiana Bertoni; Family Asteraceae] is a natural sweetener plant that is grown commercially in many parts of Brazil, Paraguay, Central America, Thailand, Korea, China and India. The leaves of stevia are the source of sweet glycosides. It is mainly used as a sweetener and flavor enhancer in the food and ...

  14. Captación y uso de la radiación en plantas de estevia (Stevia rebaudiana Bert. en el Caribe colombiano

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    Alfredo Jarma O

    2010-01-01

    Full Text Available La captación y uso eficiente de la radiación en estevia, especie productora de edulcorantes no calóricos, es un aspecto fundamental de su explotación en regiones cálidas del Neotrópico colombiano. Sin embargo, los trabajos sobre estos aspectos son escasos. La presente investigación se realizo en el municipio de Montería (Córdoba, Colombia durante las estaciones secas de 2007 y 2008 (E1 y E2, para determinar el efecto de la radiación fotosintéticamente activa (RFA sobre la producción de biomasa de los genotipos Morita 1 (M1 y Morita 2 (M2, establecidos a densidades de siembra de 25,00; 12,50 y 6,25 plantas/m². En ambas estaciones se uso un diseño experimental de bloques completos al azar con arreglo factorial 2 x 3. Se utilizo un modelo matemático que calculó la tasa de acumulación diaria de masa seca (MS en hojas y tallo (simulada con el método de integración de Euler, a partir de la RFA, la fracción de la RFA interceptada por el dosel (FLINT y el uso eficiente de la radiación (UER. En E1 se presentaron en promedio 3 MJ m-2 dia-1 mas que en E2, y un desarrollo del índice de área foliar IAF (3,12±0,02 que fue casi tres veces el registrado en E2. Consecuentemente, el valor mas alto del coeficiente de extinción de luz a través del dosel (k = 0,21 lo registro M2 durante la estación E1, a la densidad de 25 plantas/m². Durante este mismo periodo se observaron los valores mas altos de UER (2,04 g MJ RFA y el máximo porcentaje de distribución de MS total hacia las hojas, que fue del 60% en M2

  15. Ekstraksi Gula Stevia Dari Tanaman Stevia Rebaudiana Bertoni

    OpenAIRE

    Ratnani, R. D; Anggraeni, R

    2005-01-01

    Stevia Rebaudiana Bertoni adalah sejenis Tanaman perdu yang belum banyak dikenal oleh masyarakat. Didalam Industri, banyak digunakan sebagai bahan pemanis untuk produk makanan dan minuman berkalori rendah atau sebagai pengganti gula bagi penderita diabetes.Proses pengambilan bahan pemanis dari daun Stevia Rebaudiana dilakukan dengan cara ekstraksi bubuk daun didalam pelarut ( metanol,etanol ,spiritus , aquadest ) yang dilanjutkan dengan pencucian menggunakan Khloroform.Kemudian dicuci kemb...

  16. Fisiología de estevia (stevia rebaudiana) en función de la radiación en el caribe colombiano. ii. análisis de crecimiento

    OpenAIRE

    Jarma, Alfredo; Rengifo, Teresita; Araméndiz-Tatis, Hermes

    2010-01-01

    Stevia rebaudiana Bertoni es una plantaque produce en las hojas un edulcorante natural bajo en calorías, cuyo poder es 300 veces mayor que la sacarosa. El edulcorante es usado en productos alimenticios en varios países, incluyendo Japón, Brasil y China, y últimamente ha despertado gran interés en Colombia. Este trabajo se realizó entre 2002 y 2003 en la Facultad de Ciencias Agrícolas de la Universidad de Córdoba, municipio de Montería (Colombia). El propósito fue evaluar el efecto de cuatro n...

  17. Diterpene Glycosides from Stevia rebaudiana

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    Indra Prakash

    2011-04-01

    Full Text Available Three novel diterpene glycosides were isolated for the first time from the commercial extract of the leaves of Stevia rebaudiana, along with several known steviol glycosides, namely stevioside, rebaudiosides A-F, rubusoside and dulcoside A. The new compounds were identified as 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyloxy] ent-kaur-15-en-19-oic acid (1, 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyloxy]-16β-hydroxy-ent-kauran-19-oic acid (2 and 13-methyl-16-oxo-17-nor-ent-kauran-19-oic acid-β-D-glucopyranosyl ester (3 on the basis of extensive 2D NMR and MS spectroscopic data as well as chemical studies.

  18. Diterpene glycosides from Stevia rebaudiana.

    Science.gov (United States)

    Chaturvedula, Venkata Sai Prakash; Upreti, Mani; Prakash, Indra

    2011-04-28

    Three novel diterpene glycosides were isolated for the first time from the commercial extract of the leaves of Stevia rebaudiana, along with several known steviol glycosides, namely stevioside, rebaudiosides A-F, rubusoside and dulcoside A. The new compounds were identified as 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-15-en-19-oic acid, 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]-16β-hydroxy-ent-kauran-19-oic acid and 13-methyl-16-oxo-17-nor-ent-kauran-19-oic acid-β-D-glucopyranosyl ester on the basis of extensive 2D NMR and MS spectroscopic data as well as chemical studies.

  19. Estevia, el edulcorante natural.

    OpenAIRE

    Larrañaga, Nerea; Mendiola Ubillos, María Ángeles; Martinez Laborde, Juan Bautista

    2011-01-01

    La estevia, Stevia rebaudiana (Bertoni) Bertoni, es una planta herbácea y perenne descrita por primera vez en 1889 por el naturalista Moisés Bertoni. Se trata de una especie originaria de la Región Oriental de Paraguay, donde los indígenas guaraníes la han usado tradicionalmente por sus aplicaciones edulcorantes y medicinales. La hoja fresca de estevia es de 10 a 15 veces más dulce que el azúcar común, la hoja seca y pulverizada lo es unas 70, mientras que los extractos lo son hasta 200 ó 300...

  20. The Importance of Stevia (Stevia Rebaudiana Bertoni) in Public Health

    OpenAIRE

    Esra UCAR

    2016-01-01

    In the absence of modern medicine, people used alternative medicine for the treatment of diseases and they are still in use. Recently, due to the side effects caused by the drug, people prefer are turning to natural and so interest in alternative medicine is increasing with each passing day. The plants and their active ingredients constitute the basis of the alternative medicine. Stevia is also among the plants used for this purpose. Stevia rebaudiana Bertoni that is a perennial plant belongs...

  1. In-vitro Antimicrobial and Antitumor Activities of Stevia Rebaudiana ...

    African Journals Online (AJOL)

    Purpose: The purpose of the study is to evaluate the antimicrobial and antitumor activities of Stevia Rebaudiana (Asteraceae) leaf extracts. Methods: Four solvent extracts (ethyl acetate, acetone, chloroform and water) of Stevia rebaudiana leaves were investigated against Staphylococcus aureus, Salmonella typhi, ...

  2. Safety and efficacy of health supplement (Stevia rebaudiana)

    OpenAIRE

    Mazlina Mukhtar; Chua Say Tiong; Sarah I. Bukhari; Amir Heberd Abdullah; Long Chiau Ming

    2016-01-01

    Purpose: The aim of this review is to evaluate the safety and efficacy of a product containing Stevia rebaudiana and its related marketing restriction governed by laws. Methods: An electronic search was conducted through searching at Cochrane Library, EMBASE, PUBMED to assess the literature of Stevia rebaudiana. Results: Stevia was considered safe to be used as sugar substitution in patients with diabetes. No serious adverse events and health risk were reported. However, until now, most...

  3. Fisiología de estevia (Stevia rebaudiana en función de la radiación en el Caribe colombiano. II. Análisis de crecimiento

    Directory of Open Access Journals (Sweden)

    Jarma Alfredo

    2006-06-01

    Ciencias Agrícolas de la Universidad de Córdoba, municipio de Montería (Colombia. El propósito fue evaluar el efecto de cuatro niveles de radiación incidente sobre el crecimiento de Stevia rebaudiana bajo condiciones climáticas del valle del Sinú. Para lograr los objetivos se planteó un diseño completamente al azar con arreglo factorial, en el que los factores fueron niveles de radiación incidente (19%, 24%, 56% y 100% y genotipos de estevia (‘Morita 1’ y ‘Morita 2’. Los resultados más importantes indicaron que ‘Morita 2’ expuesta a 100% de radiación incidente presentó la máxima tasa absoluta de crecimiento (TAC, lo que podría atribuirse a una ventaja genética de esta variedad que le permite realizar mayor fotosíntesis. La tasa relativa de crecimiento (TRC fue alta en ambos genotipos al inicio del ciclo para los niveles sombreados (19% y 24%; una vez terminada esta etapa, las altas radiaciones generaron mayores incrementos en la TRC. La mayor tasa de asimilación neta (TAN la presentó ‘Morita 2’ expuesta a 100% de radiación incidente,  sugiriendo mayor eficiencia en la producción diaria de biomasa por cada centímetro cuadrado de superficie foliar.

  4. A New Diterpene Glycoside from Stevia rebaudiana

    Directory of Open Access Journals (Sweden)

    Indra Prakash

    2011-04-01

    Full Text Available From the commercial extract of the leaves of Stevia rebaudiana, a new diterpene glycoside was isolated besides the known steviol glycosides including stevioside, rebaudiosides A-F, rubusoside and dulcoside A. The new compound was identified as 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyloxy] ent-kaur-16-en-19-oic acid-(2-O-α-L-rhamnopyranosyl-β-D-glucopyranosyl ester (1 on the basis of extensive spectroscopic (NMR and MS and chemical studies.

  5. A new diterpene glycoside from Stevia rebaudiana.

    Science.gov (United States)

    Chaturvedula, Venkata Sai Prakash; Prakash, Indra

    2011-04-04

    From the commercial extract of the leaves of Stevia rebaudiana, a new diterpene glycoside was isolated besides the known steviol glycosides including stevioside, rebaudiosides A-F, rubusoside and dulcoside A. The new compound was identified as 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-(2-O-α-L-rhamnopyranosyl-β-D-glucopyranosyl) ester (1) on the basis of extensive spectroscopic (NMR and MS) and chemical studies.

  6. A New Diterpene Glycoside from Stevia rebaudiana

    OpenAIRE

    Indra Prakash; Venkata Sai Prakash Chaturvedula

    2011-01-01

    From the commercial extract of the leaves of Stevia rebaudiana, a new diterpene glycoside was isolated besides the known steviol glycosides including stevioside, rebaudiosides A-F, rubusoside and dulcoside A. The new compound was identified as 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-(2-O-α-L-rhamnopyranosyl-β-D-glucopyranosyl) ester (1) on the basis of extensive spectroscopic (NMR and MS) and chemical studies.

  7. Nutritional Composition of Stevia rebaudiana Bertoni Leaf: Effect of ...

    African Journals Online (AJOL)

    ... that Stevia leaves is a good source of carbohydrate and other nutrients and hence a substitute for sugar in processed drinks. Furthermore, drying reduces nutritional values with the exception to fiber content. Keywords: Stevia rebaudiana Bertoni , Nutritional composition, Protein, Heavy metals, Drying, Total energy ...

  8. Study of Stevia rebaudiana Bertoni antioxidant activities and cellular properties.

    Science.gov (United States)

    Bender, Cecilia; Graziano, Sara; Zimmermann, Benno F

    2015-01-01

    The aim of our study was to determine the antioxidant activities, cytotoxicity and proliferative properties in Stevia rebaudiana leaves and stems. Leaves extracts exhibited a higher antioxidant activity than stems extract, through oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity (CAA) assays. Stevioside and rebaudioside A, the main sweetening metabolites in stevia leaves, exhibited a low ORAC value in comparison with plant extracts, while did not elicit any CAA. Stevia rebaudiana did not exhibit toxicity against HepG2 (hepatocellular carcinoma) human cells. No proliferative nor catalase modulations were observed in cells treated with such extracts. Our findings support the promising role of stevia that, apart from its sweetness, can act as a source of antioxidants, even at the intracellular level. This activity makes S. rebaudiana crude extract an interesting resource of natural sweetness with antioxidant properties which may find numerous applications in foods and nutritional supplements industries.

  9. Stevia

    Science.gov (United States)

    Stevia (Stevia rebaudiana) is a bushy shrub that is native to northeast Paraguay, Brazil and Argentina. It is now ... a source of natural sweeteners. Some people take stevia by mouth for medical purposes such as lowering ...

  10. Stevia rebaudiana Bertoni - chemical composition and functional properties.

    Science.gov (United States)

    Marcinek, Katarzyna; Krejpcio, Zbigniew

    2015-01-01

    Sweetleaf (Stevia rebaudiana Bertoni), currently investigated by many researchers, has been known and used for more than a thousand years indigenous tribes of South America, who called it "kaa-hee" ("sweet herb"). Thanks to its chemical composition and processability sweetleaf may be an alternative for synthetic sweeteners. Nutritional and health-promoting aspects of Stevia rebaudiana are presently being studied in many research centres. The aim of this study is to present nutritional and health-promoting value of the still-little known sweetleaf.

  11. In Vitro Antimicrobial Activity of Stevia Rebaudiana Bertoni Leaves

    African Journals Online (AJOL)

    Clinical Phamacy

    and their antimicrobial activities were examined against few selected microorganisms including B. ... screening of them may result in the discovery of ... rebaudiana leaves against the selected microorganisms. MATERIALS AND METHODS. Plant Material and Microorganisms: Dried stevia leaves were supplied by Growmore.

  12. Utilisation of steviol glycosides from Stevia rebaudiana (Bertoni) by lactobacilli and bifidobacteria in in vitro conditions

    National Research Council Canada - National Science Library

    Kunová, Gabriela; Rada, Vojtěch; Vidaillac, Adrien; Lisova, Ivana

    2014-01-01

    ... from the sweetener Natusweet M001 originating from herb Stevia rebaudiana (Bertoni). Stevia is gaining popularity as a natural, non-caloric sugar substitute, and recently, it was allowed as a food additive by European Union too...

  13. Stevia rebaudiana (Bert.) Bertoni. UNA REVISIÓN

    OpenAIRE

    Michel Martínez Cruz

    2015-01-01

    Stevia (Stevia rebaudiana(Bert.)Bertoni) esunaespeciedel géneroSteviade la familia de lasAsteráceasnativa de la regióntropicaldeSudamérica.Es una plantaconsiderada medicinal, pues varios estudios demuestran quepuede tener efectos beneficiosos sobre la diabetes tipo II,ya que posee glicósidos con propiedades edulcorantes sincalorías. Muchas de las actividades de investigación sobre suspropiedades químicas y biológicas se han hecho en el pasadoreciente. El objetivo de este trabajo fue compilar ...

  14. Identification, Quantification, and Sensory Characterization of Steviol Glycosides from Differently Processed Stevia rebaudiana Commercial Extracts

    NARCIS (Netherlands)

    Espinoza, M.I.; Vincken, J.P.; Sanders, M.G.; Castro, C.; Stieger, M.A.; Agosin, E.

    2014-01-01

    Stevia rebaudiana is known for its sweet-tasting ent-kaurene diterpenoid glycosides. Several manufacturing strategies are currently employed to obtain Stevia sweeteners with the lowest possible off-flavors. The chemical composition of four commercial S. rebaudiana extracts, obtained by different

  15. Profiling and Quantification of Phenolics in Stevia rebaudiana Leaves.

    Science.gov (United States)

    Karaköse, Hande; Müller, Anja; Kuhnert, Nikolai

    2015-10-21

    Stevia rebaudiana (Bertoni) is a plant from the Asteraceae family with significant economic value because of the steviol glycoside sweeteners in its leaves. Chlorogenic acids and flavonoid glycosides of S. rebaudiana from seven different botanical varieties cultivated over two years and harvested three times a year in eight European locations were profiled and quantified in a total of 166 samples. Compounds quantified include chlorogenic acids as well as flavonoid glycosides and aglycons. All phenolic concentration profiles show a perfect Gaussian distribution. Principal component analyses allow distinction between varieties of different geographical origin and distinction between different plant varieties. Although concentrations of all chlorogenic acids showed a positive correlation, no correlation was observed for flavonoid glycosides. Conclusions from these findings with respect to the biosynthesis and functional role of phenolics in S. rebaudiana are discussed.

  16. Is Stevia rebaudiana Bertoni a Non Cariogenic Sweetener? A Review.

    Science.gov (United States)

    Ferrazzano, Gianmaria Fabrizio; Cantile, Tiziana; Alcidi, Brunella; Coda, Marco; Ingenito, Aniello; Zarrelli, Armando; Di Fabio, Giovanni; Pollio, Antonino

    2015-12-26

    Stevia rebaudiana Bertoni is a small perennial shrub of the Asteraceae (Compositae) family that is native to South America, particularly Brazil and Paraguay, where it is known as "stevia" or "honey leaf" for its powerful sweetness. Several studies have suggested that in addition to their sweetness, steviosides and their related compounds, including rebaudioside A and isosteviol, may offer additional therapeutic benefits. These benefits include anti-hyperglycaemic, anti-hypertensive, anti-inflammatory, anti-tumor, anti-diarrheal, diuretic, and immunomodulatory actions. Additionally, critical analysis of the literature supports the anti-bacterial role of steviosides on oral bacteria flora. The aim of this review is to show the emerging results regarding the anti-cariogenic properties of S. rebaudiana Bertoni. Data shown in the present paper provide evidence that stevioside extracts from S. rebaudiana are not cariogenic. Future research should be focused on in vivo studies to evaluate the effects on dental caries of regular consumption of S. rebaudiana extract-based products.

  17. Biological activity of Stevia rebaudiana Bertoni and their relationship to health.

    Science.gov (United States)

    Ruiz-Ruiz, Jorge Carlos; Moguel-Ordoñez, Yolanda Beatriz; Segura-Campos, Maira Rubi

    2017-08-13

    The leaves of Stevia rebaudiana Bertoni has nutrients and phytochemicals, which make it an adequate source for the extraction and production of functional food ingredients. Preclinical and clinical studies suggest therapeutic and pharmacological applications for stevia and their extracts because they are not toxic and exhibit several biological activities. This review presents the biological activity of Stevia rebaudiana Bertoni and their relationship to antidiabetic, anticariogenic, antioxidant, hypotensive, antihypertensive, antimicrobial, anti-inflammatory and antitumor activities. Consumption and adverse effects were also reviewed.

  18. Produksi Low Calorie Sweet Bio-Yoghurt dengan Penambahan Ekstrak Daun Stevia (Stevia Rebaudiana) sebagai Pengganti Gula

    OpenAIRE

    Widodo, Widodo; Munawaroh, Naimatun; Indratiningsih, Indratiningsih

    2015-01-01

    Penelitian ini bertujuan untuk mengkaji pemanfaatan stevioside hasil ekstraksi daun stevia (Stevia rebaudiana) sebagai pengganti gula dalam produk low calorie sweet bio-yoghurt. Penelitian dilakukan dengan penambahan 0,5; 2,0 dan 3,5% ekstrak daun stevia pada yoghurt dan sebagai kontrol yoghurt dengan penambahan 7,0% gula. Parameter yang diamati adalah kadar stevioside hasil ekstraksi, kandungan kalori, nilai pH dan keasaman, komposisi nutrisi, kualitas mikrobiologis, serta sensoris produk yo...

  19. Polyhouse cultivation of invitro raised elite Stevia rebaudiana Bertoni: An assessment of biochemical and photosynthetic characteristics

    Science.gov (United States)

    Polyhouse cultivated Stevia rebaudiana Bertoni plants, initially raised from synthetic seeds, were assessed for biochemical and photosynthetic characteristics and compared with their mother plant. Synthetic seeds were produced using nodal segments containing single axillary buds excised from in vitr...

  20. CHEMICAL CHARACTERIZATION OF THE STEVIA REBAUDIANA

    OpenAIRE

    Bravo A., M.; Departamento de Química Analítica,FQIQ Universidad Nacional Mayor de San Marcos Lima, Perú; Ale B., N.; Departamento de Química Analítica, FQIQ, Universidad Nacional Mayor de San Marcos, Lima, Perú; Rivera C., D.; Departamento de Química Analítica, FQIQ, Lima, Perú; Huamán M., J.; Departamento de Química Orgánica, FQIQ, Universidad Nacional Mayor de San Marcos, Lima, Perú; Delmás R., D.; Departamento de Química Analítica, FQIQ, Lima, Perú; Rodríguez B., M.; Departamento de Química Analítica, FQIQ, Universidad Nacional Mayor de San Marcos, Lima, Perú; Polo S., M.; Departamento de Química Inorgánica, FQIQ, Lima, Perú; Bautista C., M.; Departamento de Procesos, FQIQ, Universidad Nacional Mayor de San Marcos, Lima, Perú

    2014-01-01

    Aqueous extractions were performed controlling the pH with CaCO3 and Ca(OH)2, using as clarifiers activated coal and activated clays in cold and warm solutions to obtain best results with cold bentonite, warm activated clay and warm activated coal. Analytical results were compared with a commercial sample of stevia. Se realizaron extracciones acuosas, controlando el pH con CaCO3 e Ca(OH)2, se utilizaron como clarificantes carbón activado y arcillas activadas en soluciones frías y calientes...

  1. Antioxidant properties of cell wall polysaccharides of Stevia rebaudiana leaves

    Directory of Open Access Journals (Sweden)

    Mediesse Kengne Francine

    2014-12-01

    Full Text Available Objective: To examine the total phenolic and protein contents, and the antioxidant activities of cell wall polysaccharide fractions of Stevia rebaudiana leaves. Methods: Three different polysaccharide-enriched fractions, namely FPE (extract with 50 mmol/ L ethylene diamine tetra acetic acid, FPK (extract with 0.05 mol/L KOH and FH (extract with 4 mol/L KOH were extracted from Stevia rebaudiana leaves. The antioxidant activity of these fractions was evaluated based on their ability to scavenge DPPH (1, 1-diphenyl-2-picryl hydrazyl free radical, to reduce ferric power, to chelate ferrous ion and to protect human DNA. Results: The results indicated that protein content was found to be higher in FPK polysaccharide enriched fraction (47.48 µg per mg of FPK. Furthermore, the phenolic compound analysis according to the Folin-Ciocalteu method was higher in FPK (17.71 µg ferulic acid. The DPPH maximal inhibition percentage of the three polysaccharide-enriched fractions at 400 µg/mL was 27.66%, 59.90% and 23.21% respectively for FPE, FPK and FH. All the polysaccharide fractions exhibited a ferric reducing power except the FH one. The three fractions also exhibited lipid peroxidation inhibition, and they completely reverted the DNA damage induced by H2O2/FeCl2. FPK showed the strongest scavenging activity against the DPPH radical, the best chelating ability and lipid peroxidation inhibition. Conclusions: Stevia cell wall polysaccharide fractions are potent protective agents against oxidative stress. The analysis revealed major differences in the antioxidant activity in the three polysaccharides fractions. However, the 0.05 mol/L KOH pectin fraction (FPK showed better antioxidant activity.

  2. Purple Phototrophic Bacterium Enhances Stevioside Yield by Stevia rebaudiana Bertoni via Foliar Spray and Rhizosphere Irrigation

    OpenAIRE

    Jing Wu; Yiming Wang; Xiangui Lin

    2013-01-01

    This study was conducted to compare the effects of foliar spray and rhizosphere irrigation with purple phototrophic bacteria (PPB) on growth and stevioside (ST) yield of Stevia. rebaudiana. The S. rebaudiana plants were treated by foliar spray, rhizosphere irrigation, and spray plus irrigation with PPB for 10 days, respectively. All treatments enhanced growth of S. rebaudiana, and the foliar method was more efficient than irrigation. Spraying combined with irrigation increased the ST yield pl...

  3. In vitro rapid multiplication of Stevia rebaudiana: an important natural sweetener herb

    Directory of Open Access Journals (Sweden)

    RAVINDRA ADE

    2012-11-01

    Full Text Available Deshmukh S, Ade R. 2012. In vitro rapid multiplication of Stevia rebaudiana: an important natural sweetener herb. Nusantara Bioscience 4: 105-108. Stevia rebaudiana Bertoni, belonging to family Asteraceae and natural sweet plant, but due to poor seed viability, fertility and vigor, Stevia cultivation is a challenging task. In the present study in vitro rapid multiplication method was established for Stevia rebaudiana by inoculating explants on M.S. medium, supplemented with different combination of phytoharmone. The maximum number of shoots (18.3±0.8 was obtained on M.S. medium supplemented with BAP + KIN (1.5 + 0.5 mg/L. The highest rooting percentage (95.25 was observed with (IAA 0.1 mg/L. The rooted plants were successfully established firstly in soil with coco peat (1:1 and then directly in ordinary soil.

  4. Structures of the novel diterpene glycosides from Stevia rebaudiana.

    Science.gov (United States)

    Chaturvedula, Venkata Sai Prakash; Prakash, Indra

    2011-06-01

    From the commercial extract of the leaves of Stevia rebaudiana, two new diterpenoid glycosides were isolated besides the known steviol glycosides including stevioside, rebaudiosides A-F, rubusoside, and dulcoside A. The structures of the two new compounds were identified as 13-[(2-O-6-deoxy-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid β-d-glucopyranosyl ester (1), and 13-[(2-O-6-deoxy-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid β-d-glucopyranosyl ester (2), on the basis of extensive NMR and MS spectral data as well as chemical studies. Copyright © 2011 Elsevier Ltd. All rights reserved.

  5. Stevia rebaudiana (Bert.) Bertoni extract clarification by cactus Cereus peruvianus

    OpenAIRE

    Fernandes, Larissa Maria; UEM; Pereira, Nehemias Curvelo; UEM; Mendes, Elisabete Scolin; UEM; da Motta Lima, Oswaldo Curty; UEM; da Costa, Silvio Claudio; UEM

    2008-01-01

    A tendência das indústrias que se preocupam em promover a saúde e prevenir doenças está em investir no desenvolvimento de tecnologias para produção de alimentos com baixas calorias e reduzido teor de gordura, mantendo suas qualidades nutricionais. Um dos estudos mais freqüentes é a substituição do açúcar por edulcorantes não calóricos, com a preocupação que estes substitutos sejam semelhantes em função organoléptica. Nas folhas da planta conhecida como Stevia rebaudiana (Bert.) Bertoni (Aster...

  6. In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia

    Directory of Open Access Journals (Sweden)

    Ummi Nur Ain Abdul Razak

    2014-02-01

    Full Text Available Stevia rebaudiana Bertoni is a medicinal plants and commercially use as non-caloric sweetener for diabetic patient. In the present study, a protocol was developed for in vitro micropropagation using 6-benzylamino purine (BAP and Kinetin (Kn for the formation of multiple shoot proliferation and Indole-3-acetic acid (IAA, Indole-3-butyric acid (IBA and 1-Naphthaleneacetic acid (NAA for the induction of roots. Maximum shoot formation (7.82 ± 0.7 shoots per explants was observed on a Murashige and Skoog (MS medium supplemented with 0.5 mg L-1 BAP and 0.25 mg L-1 Kn. The maximum number of roots (30.12 ± 2.1 roots per explants was obtained on a MS medium containing 1.0 mg L-1 IBA. The well rooted plantlets were successfully weaned and acclimatized in plant soil with survival rate of 83.3 %.

  7. Biosynthesis of silver nanoparticles using leaves of Stevia rebaudiana

    Energy Technology Data Exchange (ETDEWEB)

    Yilmaz, M. [Department of Metallurgy and Materials Engineering, Faculty of Engineering, Bartin University, Bartin (Turkey); Turkdemir, H. [Department of Chemistry, Faculty of Arts and Sciences, Uludag University, 16059 Goeruekle, Bursa (Turkey); Kilic, M. Akif [Department of Biology, Faculty of Science, Akdeniz University, Campus 07058, Antalya (Turkey); Bayram, E. [Department of Chemistry, Faculty of Science, Akdeniz University, Campus 07058, Antalya (Turkey); Cicek, A. [Department of Physics, Faculty of Arts and Sciences, Mehmet Akif Ersoy University, 15100 Burdur (Turkey); Department of Physics, Faculty of Science, Akdeniz University, Campus 07058, Antalya (Turkey); Mete, A. [Department of Chemistry, Faculty of Arts and Sciences, Inonu University, Malatya (Turkey); Ulug, B., E-mail: bulug@akdeniz.edu.tr [Department of Physics, Faculty of Science, Akdeniz University, Campus 07058, Antalya (Turkey)

    2011-11-01

    Highlights: {yields} Green synthesis of silver nanoparticles using leaves of Stevia Rebaudiana. {yields} Spherical and polydispersed nanoparticles with diameters below 50 nm. {yields} Interplay of nanoparticle formation and aggregation over time. {yields} Capping reagents similar to those in gold synthesis via the same biomass. {yields} Ketones to play active roles in the reduction of silver ions. - Abstract: The synthesis of silver nanoparticles employing a shadow-dried Stevia rebaudiana leaf extract in AgNO{sub 3} solution is reported. Transmission electron microscopy and X-ray diffraction inspections indicate that nanoparticles are spherical and polydispersed with diameters ranging between 2 and 50 nm with a maximum at 15 nm. Ultraviolet-visible spectra recorded against the reaction time confirms the reduction of silver nanoparticles indicating that the formation and the aggregation of nanoparticles take place shortly after the mixing, as they persist concurrently with characteristic times of 48.5 min and 454.5 min, respectively. Aggregation is found to be the dominant mechanism after the first 73 min. Proton nuclear magnetic resonance spectrum of the silver nanoparticles reveals the existence of aliphatic, alcoholic and olefinic CH{sub 2} and CH{sub 3} groups, as well as some aromatic compounds but no sign of aldehydes or carboxylic acids. Infrared absorption of the silver nanoparticles suggests that the capping reagents of silver and gold nanoparticles reduced in plant extracts/broths are of the same chemical composition of different ratios. Ketones are shown to play a somehow active role for the formation of nanoparticles in plant extracts/broths.

  8. Additional Minor Diterpene Glycosides from Stevia rebaudiana Bertoni

    Directory of Open Access Journals (Sweden)

    Indra Prakash

    2013-10-01

    Full Text Available Two additional novel minor diterpene glycosides were isolated from the commercial extract of the leaves of Stevia rebaudiana Bertoni. The structures of the new compounds were identified as 13-{β-D-glucopyranosyl-(1→2-O-[β-D-glucopyranosyl-(1→3-β-D-glucopyranosyl-oxy} ent-kaur-16-en-19-oic acid {β-D-xylopyranosyl-(1→2-O-[β-D-glucopyranosyl-(1→3]-O-β-D-glucupyranosyl-ester} (1, and 13-{β-D-6-deoxy-glucopyranosyl-(1→2-O-[β-D-glucopyranosyl-(1→3-β-D-glucopyranosyl-oxy} ent-kaur-16-en-19-oic acid {β-D-glucopyranosyl-(1→2-O-[β-D-glucopyranosyl-(1→3-β-D-gluco-pyranosyl-ester} (2, on the basis of extensive 1D (1H- and 13C- 2D NMR (COSY, HSQC and HMBC and MS spectroscopic data as well as chemical studies.

  9. Nutritional, amylolytic enzymes inhibition and antioxidant properties of bread incorporated with Stevia rebaudiana.

    Science.gov (United States)

    Ruiz-Ruiz, Jorge C; Moguel-Ordoñez, Yolanda B; Matus-Basto, Angel J; Segura-Campos, Maira R

    2015-01-01

    Wheat bread with sucrose content replaced with different levels of stevia extract was compared with traditional wheat bread. The ability to reduce glucose intake was highlighted by performing enzymatic assays using α-amylase and α-glucosidase. Antioxidant activity was measured by determining the scavenging effect on α,α-diphenyl-β-picrylhydrazyl radical. In comparison with the control, the bread with stevia extract was softer and had lower microbial growth during the shelf-life study. The sensory test showed that the substitution of 50% stevia extract was more acceptable when comparing with all the quality characteristics. Regarding the nutritional contribution, the content of dietary fiber and digestible carbohydrates in the bread with stevia extract was higher and lower respectively, so caloric intake was significantly reduced. The results showed that the biological properties of Stevia rebaudiana extract were retained after the bread making process and that the proposed bread could be suitable as functional food in human nutrition.

  10. Agronomic-productive characteristics of two genotype of stevia rebaudiana in Central Italy

    Directory of Open Access Journals (Sweden)

    Lucia Ceccarini

    2006-06-01

    Full Text Available Stevia rebaudiana Bertoni produces a variety of high-potency low calorie sweeteners in its’ leaf tissue. The aim of this work was to evaluate the productive potential of two different Stevia rebaudiana genotypes and the characteristics of the production of the plant obtained through different cultivation methods, in central Italy. For several years (1992-2000 agronomic trials on Stevia rebaudiana cultivated in the littoral area near Pisa (Italy carried out. In 1992 two different genotypes of Stevia (B1 and B2 transplanted in silt-loam soil; in 1996 other plants from B2 genotype, produced by vegetative process (micropropagation, were transplanted in the same field. In the period from 1992-1999 the production of leaves from both genotypes increase as of the third year and the ratio leaf-aerial plant, after the peak in the third year, tends to diminish. Except the first year (1997 there were not recorded statistically significant differences between the two propagation methods employed in relation to leaf production. The quantity of leaves produced from a single harvest was less than resulting from two cuttings and the micropropagation plants produced a larger amount of leaves than those from cutting. The leaf-stem ratio was to become an interesting morphological and production characteristic parameter of the plant. Stevia rebaudiana appears particularly suited for the cultivation environment of central Italy. A particular positive aspect that must be considered in these regions is that Stevia can be grown successfully as poliannual species because crop survival over the winter is high. The results obtained show that this species is economically profitable until the 5th or 6th year of cultivation. At our latitudes is also necessary to establish a program of genetic improvement in order to develop earlier varieties that can guaranty an optimum qualitative and quantitative seed production.

  11. Stevia rebaudiana targeting α-amylase: An in-vitro and in-silico mechanistic study.

    Science.gov (United States)

    Singla, Ramit; Singla, Navdeep; Jaitak, Vikas

    2017-10-26

    Diabetes mellitus (DM) is the fastest growing metabolic disorder in the world. Recently, more attention is paid to the study of natural products due to side effects of synthetic drugs. Stevia rebaudiana (Bertoni) is considered an encouraging starting point for the antidiabetic lead development. In the present study, the in vitro α-amylase inhibitory activity of the extracts of S. rebaudiana is investigated. In order to understand the molecular mechanism and future pharmacophore development, in silico study of secondary metabolites isolated from S. rebaudiana was carried out. Results indicated that water extract shows highest α-amylase inhibitory activity as compared to other extracts. Moreover, compound 20 (rebaudioside A) which has been previously reported and isolated from water extract showed the impressive binding profile with α-amylase. Therefore, our study suggests that S. rebaudiana could be used in the development of therapeutic drugs for the treatment of diabetes.

  12. Purple phototrophic bacterium enhances stevioside yield by Stevia rebaudiana Bertoni via foliar spray and rhizosphere irrigation.

    Science.gov (United States)

    Wu, Jing; Wang, Yiming; Lin, Xiangui

    2013-01-01

    This study was conducted to compare the effects of foliar spray and rhizosphere irrigation with purple phototrophic bacteria (PPB) on growth and stevioside (ST) yield of Stevia. rebaudiana. The S. rebaudiana plants were treated by foliar spray, rhizosphere irrigation, and spray plus irrigation with PPB for 10 days, respectively. All treatments enhanced growth of S. rebaudiana, and the foliar method was more efficient than irrigation. Spraying combined with irrigation increased the ST yield plant (-1) by 69.2% as compared to the control. The soil dehydrogenase activity, S. rebaudiana shoot biomass, chlorophyll content in new leaves, and soluble sugar in old leaves were affected significantly by S+I treatment, too. The PPB probably works in the rhizosphere by activating the metabolic activity of soil bacteria, and on leaves by excreting phytohormones or enhancing the activity of phyllosphere microorganisms.

  13. Purple phototrophic bacterium enhances stevioside yield by Stevia rebaudiana Bertoni via foliar spray and rhizosphere irrigation.

    Directory of Open Access Journals (Sweden)

    Jing Wu

    Full Text Available This study was conducted to compare the effects of foliar spray and rhizosphere irrigation with purple phototrophic bacteria (PPB on growth and stevioside (ST yield of Stevia. rebaudiana. The S. rebaudiana plants were treated by foliar spray, rhizosphere irrigation, and spray plus irrigation with PPB for 10 days, respectively. All treatments enhanced growth of S. rebaudiana, and the foliar method was more efficient than irrigation. Spraying combined with irrigation increased the ST yield plant (-1 by 69.2% as compared to the control. The soil dehydrogenase activity, S. rebaudiana shoot biomass, chlorophyll content in new leaves, and soluble sugar in old leaves were affected significantly by S+I treatment, too. The PPB probably works in the rhizosphere by activating the metabolic activity of soil bacteria, and on leaves by excreting phytohormones or enhancing the activity of phyllosphere microorganisms.

  14. Purple Phototrophic Bacterium Enhances Stevioside Yield by Stevia rebaudiana Bertoni via Foliar Spray and Rhizosphere Irrigation

    Science.gov (United States)

    Wu, Jing; Wang, Yiming; Lin, Xiangui

    2013-01-01

    This study was conducted to compare the effects of foliar spray and rhizosphere irrigation with purple phototrophic bacteria (PPB) on growth and stevioside (ST) yield of Stevia. rebaudiana. The S. rebaudiana plants were treated by foliar spray, rhizosphere irrigation, and spray plus irrigation with PPB for 10 days, respectively. All treatments enhanced growth of S. rebaudiana, and the foliar method was more efficient than irrigation. Spraying combined with irrigation increased the ST yield plant -1 by 69.2% as compared to the control. The soil dehydrogenase activity, S. rebaudiana shoot biomass, chlorophyll content in new leaves, and soluble sugar in old leaves were affected significantly by S+I treatment, too. The PPB probably works in the rhizosphere by activating the metabolic activity of soil bacteria, and on leaves by excreting phytohormones or enhancing the activity of phyllosphere microorganisms. PMID:23825677

  15. Ultra-HPLC method for quality and adulterant assessment of steviol glycosides sweeteners - Stevia rebaudiana and stevia products.

    Science.gov (United States)

    Wang, Yan-Hong; Avula, Bharathi; Tang, Wenzhao; Wang, Mei; Elsohly, Mahmoud A; Khan, Ikhlas A

    2015-01-01

    Stevia products are advertised as a zero-calorie sweetener. Glucose should not be an intrinsic component of this product, but it has been identified from some of stevia products in a preliminary study. An UHPLC-UV method was developed for the quantitative determination of glucose from stevia products. After stevia products reacted with 1-phenyl-3-methyl-5-pyrazolone (PMP), PMP derivatives were analysed and glucose was found in seven out of 35 products in the range 0.3-91.5% (w/w). Two products, SPR-12 and SPR-27, showed remarkable amounts of glucose at 61.6% and 91.5%, respectively. In addition, an UHPLC-UV-evaporative light-scattering detector (ELSD) method was developed for the quantitative determination of rebaudioside A, stevioside, rebaudioside D, dulcoside A and steviolbioside from Stevia rebaudiana and related products. In a 12 min run, five steviol glycosides were baseline-separated. ELSD and ultraviolet (UV) detections showed comparable results. The LC methods were validated for linearity, repeatability, accuracy, limits of detection (LOD) and limits of quantification (LOQ). For steviol glycosides, the LODs and LOQs were found to be less than 10 and 30 μg ml(-1), respectively. The RSD for intra- and inter-day analyses was less than 2.5%, and the recovery was 90-94%. For PMP derivative of glucose, the LOD and LOQ were 0.01 and 0.05 μg ml(-1), respectively. Repeatability (RSD) was less than 2.6%; recovery was 98.6-101.7%. The methods are useful for the identification, quality assurance, and adulterant assessment of S. rebaudiana and steviol glycosides sweeteners (stevia products).

  16. MICROPROPAGACIÓN MASIVA DE Stevia rebaudiana Bertoni EN SISTEMAS DE INMERSIÓN TEMPORAL

    OpenAIRE

    Silvana Alvarenga Venutolo; Tatiana Salazar Aguilar

    2015-01-01

    Stevia rebaudiana Bertoni, familia Asteraceae, es conocida como “yerba dulce” por poseer un edulcorante natural. Sus propiedades provienen de la presencia de glicósidos diterpenos denominados esteviósidos y rebaudiósidos en las hojas. El porcentaje de germinación de las semillas de S. rebaudiana es muy bajo y las plantas producidas son heterogéneas, por lo que no es conveniente para la propagación masiva en campo. El cultivo en sistemas de inmersión temporal, es una herramienta eficaz para la...

  17. Comprehensive review on agro technologies of low-calorie natural sweetener stevia (Stevia rebaudiana Bertoni): a boon to diabetic patients.

    Science.gov (United States)

    Sharma, Saurabh; Walia, Swati; Singh, Bikram; Kumar, Rakesh

    2016-04-01

    Stevia rebaudiana Bertoni is a low-calorie natural sweetener plant native to Paraguay. The leaves of stevia have sweetening compounds called steviol glycosides (SGs), which contain different marker compounds, i.e. stevioside (St), rebaudioside (Rb) A, B, C, D and E, dulcoside A and steviol biosides, which are nearly 300 times sweeter than sugar. Stevia is a better alternative to sugar in formulating food products, reducing the harmful effect of sugar and improving the nutrient properties. We have tried to compile a literature on various agronomic and management aspects which are helpful in increasing the yield and quality of stevia to be grown as a crop that will benefit farmers and industrialists. The stevioside thus obtained can be used to make different food products for sweetening purposes, which could be a boon to diabetic patients. Incorporation of different agronomic techniques like propagation method, transplanting time, intercropping, irrigation, mulching, plant geometry, pinching and harvesting time not only improve the biomass but also increase the quality of stevia. Therefore, agronomic considerations are of high priority to utilize its maximum potential. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  18. Use of the modified Gompertz equation to assess the Stevia rebaudiana Bertoni antilisterial kinetics.

    Science.gov (United States)

    Belda-Galbis, Clara Miracle; Pina-Pérez, María Consuelo; Espinosa, Josepa; Marco-Celdrán, Aurora; Martínez, Antonio; Rodrigo, Dolores

    2014-04-01

    In order to assess the antibacterial activity of Stevia rebaudiana Bertoni (Stevia), Listeria innocua growth was characterized at 37 °C, in reference medium supplemented with a leaf infusion, a crude extract, and a steviol glycosides purified extract. Experimental data were fitted to the modified Gompertz model and the antibacterial activity of Stevia was determined based on the lag time (λ) and the maximum growth rate (μmax) reached, depending on the incubation conditions. As the leaf infusion showed the most marked elongation of λ and the most marked μmax reduction, its antimicrobial effect was evaluated at different concentrations, at 37, 22 and 10 °C. According to the results obtained, in general, the lower the temperature or the higher the Stevia concentration, the longer the λ and the lower the μmax, statistically significant being the effect of reducing temperature from 37 or 22 to 10 °C, the effect of increasing Stevia concentration from 0 or 0.5 to 1.5 or 2.5% (w/v), at 37 °C, and the elongation of λ observed in presence of 1.5 and 2.5% (w/v) of Stevia, at 22 °C. These results show that Stevia could be a bacterial growth control measure if a cold chain failure occurs. Copyright © 2013 Elsevier Ltd. All rights reserved.

  19. Antioxidant, anti-diabetic and renal protective properties of Stevia rebaudiana.

    Science.gov (United States)

    Shivanna, Naveen; Naika, Mahadev; Khanum, Farhath; Kaul, Vijay K

    2013-01-01

    Stevia rebaudiana Bertoni has been used for the treatment of diabetes in, for example, Brazil, although a positive effect on antidiabetic and its complications has not been unequivocally demonstrated. This herb also has numerous therapeutic properties which have been proven safe and effective over hundreds of years. Streptozotocin is a potential source of oxidative stress that induces genotoxicity. We studied the effects of stevia leaves and its extracted polyphenols and fiber on streptozotocin induced diabetic rats. We hypothesize that supplementation of polyphenols extract from stevia to the diet causes a reduction in diabetes and its complications. Eighty Wistar rats were randomly divided into 8 groups; a standard control diet was supplemented with either stevia whole leaves powder (4.0%) or polyphenols or fiber extracted from stevia separately and fed for one month. Streptozotocin (60 mg/kg body weight, i.p) was injected to the diabetic groups on the 31st day. Several indices were analyzed to assess the modulation of the streptozotocin induced oxidative stress, toxicity and blood glucose levels by stevia. The results showed a reduction of blood glucose, ALT and AST, and increment of insulin level in the stevia whole leaves powder and extracted polyphenols fed rats compared to control diabetic group. Its feeding also reduced the MDA concentration in liver and improved its antioxidant status through antioxidant enzymes. Glucose tolerance and insulin sensitivity were improved by their feeding. Streptozotocin was also found to induce kidney damage as evidenced by decreased glomerular filtration rate; this change was however alleviated in the stevia leaves and extracted polyphenol fed groups. The results suggested that stevia leaves do have a significant role in alleviating liver and kidney damage in the STZ-diabetic rats besides its hypoglycemic effect. It might be adequate to conclude that stevia leaves could protect rats against streptozotocin induced diabetes

  20. EFECTO DE LA STEVIA REBAUDIANA BERTONI EN LA CICATRIZACIÓN GINGIVAL POST-CIRUGÍA PERIODONTAL EN PACIENTES DE LA CONSULTA PRIVADA. AREQUIPA. 2012

    OpenAIRE

    MANRIQUE RIVAÑOS, NORKA MILAGROS

    2015-01-01

    TABLAS DE INFORMACIÓN GENERAL TABLAS REFERENTES A LA CICATRIZACIÓN GINGIVAL DESPUÉS DE LA APLICACIÓN DE LA STEVIA REBAUDIANA BERTONI TABLAS REFERENTES A LA CICATRIZACIÓN GINGIVAL SIN LA APLICACIÓN DE LA STEVIA REBAUDIANA BERTONI

  1. Phytochemical screening and comparative antimicrobial potential of different extracts ofStevia rebaudiana Bertoni leaves

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    Abu Bakar Siddique

    2014-08-01

    Full Text Available Objective: To evaluate in vitro antimicrobial potential and phytochemical screening of the crude extracts of leaves of Stevia rebaudiana Bertoni. Methods: The essential oil and crude extracts were prepared by using different usual method. Antimicrobial and antifungal activities were measured by the well established methods. Results: Highest antifungal index [(12.13依0.08 mm] and lowest antifungal index [(9.13依0.04 mm] as well as highest antibacterial index [(11.89依0.07 mm] and lowest antibacterial index [(7.24依0.03 mm] were obtained for extracts B, H, A and F, respectively. Invariably extract C, E, I, J and H did not show antimicrobial activity. The extract F showed all antifungal and antibacterial activity except Bacillus cereus and Bacillus megaterium. Conclusions: The above findings support the idea that plant extracts of Stevia rebaudiana Bertoni leaves may have a role to be used as pharmaceuticals or preservatives.

  2. Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo

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    Rafael V. Reis

    Full Text Available ABSTRACT Stevia rebaudiana (Bertoni Bertoni, Asteraceae, is used as a food additive because its leaves are a source of steviol glycosides. There are examples of tissue culture based on micropropagation and phytochemical production of S. rebaudiana leaves but there are few studies on adventitious root culture of S. rebaudiana. More than 90% of the plants used in industry are harvested indiscriminately. In order to overcome this situation, the development of methodologies that employ biotechnology, such as root culture, provides suitable alternatives for the sustainable use of plants. The aim of this study was to compare morpho-anatomical transverse sections of S. rebaudiana roots grown in vitro and in vivo. The in vitro system used to maintain root cultures consisted of a gyratory shaker under dark and light conditions and a roller bottle system. Transverse sections of S. rebaudiana roots grown in vitro were structurally and morphologically different when compared to the control plant; roots artificially maintained in culture media can have their development affected by the degree of media aeration, sugar concentration, and light. GC–MS and TLC confirmed that S. rebaudiana roots grown in vitro have the ability to produce metabolites, which can be similar to those produced by wild plants.

  3. Safety and efficacy of health supplement (Stevia rebaudiana

    Directory of Open Access Journals (Sweden)

    Mazlina Mukhtar

    2016-01-01

    Conclusion: Although some countries still banned the use of stevia since lack of toxicology profile, it was credited with the various health benefits. The Joint Expert Committee on Food Additives revealed that stevia extracts containing 95% steviol glycosides are safe for human use in the range of 4 mg/kg of body weight/day.

  4. smRNAome profiling to identify conserved and novel microRNAs in Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Mandhan, Vibha; Kaur, Jagdeep; Singh, Kashmir

    2012-11-01

    MicroRNAs (miRNAs) constitute a family of small RNA (sRNA) population that regulates the gene expression and plays an important role in plant development, metabolism, signal transduction and stress response. Extensive studies on miRNAs have been performed in different plants such as Arabidopsis thaliana, Oryza sativa etc. and volume of the miRNA database, mirBASE, has been increasing on day to day basis. Stevia rebaudiana Bertoni is an important perennial herb which accumulates high concentrations of diterpene steviol glycosides which contributes to its high indexed sweetening property with no calorific value. Several studies have been carried out for understanding molecular mechanism involved in biosynthesis of these glycosides, however, information about miRNAs has been lacking in S. rebaudiana. Deep sequencing of small RNAs combined with transcriptomic data is a powerful tool for identifying conserved and novel miRNAs irrespective of availability of genome sequence data. To identify miRNAs in S. rebaudiana, sRNA library was constructed and sequenced using Illumina genome analyzer II. A total of 30,472,534 reads representing 2,509,190 distinct sequences were obtained from sRNA library. Based on sequence similarity, we identified 100 miRNAs belonging to 34 highly conserved families. Also, we identified 12 novel miRNAs whose precursors were potentially generated from stevia EST and nucleotide sequences. All novel sequences have not been earlier described in other plant species. Putative target genes were predicted for most conserved and novel miRNAs. The predicted targets are mainly mRNA encoding enzymes regulating essential plant metabolic and signaling pathways. This study led to the identification of 34 highly conserved miRNA families and 12 novel potential miRNAs indicating that specific miRNAs exist in stevia species. Our results provided information on stevia miRNAs and their targets building a foundation for future studies to understand their roles in key

  5. Stevia rebaudiana Bertoni effect on the hemolytic potential of Listeria monocytogenes.

    Science.gov (United States)

    Sansano, S; Rivas, A; Pina-Pérez, M C; Martinez, A; Rodrigo, D

    2017-06-05

    The effect of Stevia rebaudiana Bertoni on the hemolytic potential of Listeria monocytogenes was studied by means of the assessment of the Listeriolysin O (LLO) production. The three factors under study, stevia concentration in the range [0-2.5] % (w/v), incubation temperature (10 and 37°C), and exposure time (0-65h) significantly affected (p≤0.05) the hemolytic activity of L. monocytogenes. Results showed that at the lower incubation temperature the hemolytic potential of the bacterium was significantly reduced, from 100% at 37°C to 8% at 10°C (after 65h of incubation) in unsupplemented substrate (0% stevia). Irrespective of the temperature, 10 or 37°C, supplementation of the medium with stevia at 2.5 % (w/v) reduced the bacterium's hemolytic activity by a maximum of 100%. Furthermore, the time of exposure to 2.5 % (w/v) stevia concentration was also a significant factor reducing the hemolytic capability of L. monocytogenes. The possibility of reducing the pathogenic potential of L. monocytogenes (hemolysis) by exposure to stevia should be confirmed in real food matrices, opening a research niche with a valuable future impact on food safety. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. The effects of aqueous extract of stevia plant (Stevia rebaudiana) on serum concentration of vaspin and Angiopoietin-like Protein-3 in streptozotocin induced diabetic rats

    OpenAIRE

    Samad Akbarzadeh; Afshar Barghahi; Alireza Rahbar; Adel Daneshi; Saeed Najafpour Bushehri; Khalil Pourkhalili; S.Mojtaba Jafari; Najmeh Hajian; Behrooz Naeimi; Parviz Farzadinia

    2015-01-01

    Background: Considering the importance of the prevalence of diabetes which involves approximately 6% of the world's adult population, the need for low-calorie natural sweetener is felt more than ever. Recent studies have shown that hormones such as vaspin and Angiopoietin-like Protein3 (ANGPTL3) are associated with diabetes. The purpose of this study is to evaluate the effect of Stevia (Stevia rebaudiana ) plant (as a low calorie sugar) on serum concentration of vaspin and ANGPTL3 in diabetic...

  7. RNA-Seq for gene identification and transcript profiling of three Stevia rebaudiana genotypes.

    Science.gov (United States)

    Chen, Junwen; Hou, Kai; Qin, Peng; Liu, Hongchang; Yi, Bin; Yang, Wenting; Wu, Wei

    2014-07-07

    Stevia (Stevia rebaudiana) is an important medicinal plant that yields diterpenoid steviol glycosides (SGs). SGs are currently used in the preparation of medicines, food products and neutraceuticals because of its sweetening property (zero calories and about 300 times sweeter than sugar). Recently, some progress has been made in understanding the biosynthesis of SGs in Stevia, but little is known about the molecular mechanisms underlying this process. Additionally, the genomics of Stevia, a non-model species, remains uncharacterized. The recent advent of RNA-Seq, a next generation sequencing technology, provides an opportunity to expand the identification of Stevia genes through in-depth transcript profiling. We present a comprehensive landscape of the transcriptome profiles of three genotypes of Stevia with divergent SG compositions characterized using RNA-seq. 191,590,282 high-quality reads were generated and then assembled into 171,837 transcripts with an average sequence length of 969 base pairs. A total of 80,160 unigenes were annotated, and 14,211 of the unique sequences were assigned to specific metabolic pathways by the Kyoto Encyclopedia of Genes and Genomes. Gene sequences of all enzymes known to be involved in SG synthesis were examined. A total of 143 UDP-glucosyltransferase (UGT) unigenes were identified, some of which might be involved in SG biosynthesis. The expression patterns of eight of these genes were further confirmed by RT-QPCR. RNA-seq analysis identified candidate genes encoding enzymes responsible for the biosynthesis of SGs in Stevia, a non-model plant without a reference genome. The transcriptome data from this study yielded new insights into the process of SG accumulation in Stevia. Our results demonstrate that RNA-Seq can be successfully used for gene identification and transcript profiling in a non-model species.

  8. Genetic and metabolic diversity in Stevia rebaudiana using RAPD and HPTLC analysis.

    Science.gov (United States)

    Chester, Karishma; Tamboli, Ennus Tajuddin; Parveen, Rabea; Ahmad, Sayeed

    2013-06-01

    Stevia rebaudiana Bertoni (Asteraceae) is an important medicinal plant and is much used due to its zero calories sweetening property. Stevia leaves as well as its extracts and pure compounds are currently used in the preparation of several medicines, food products and neutraceuticals. To study the genetic and metabolic variability in S. rebaudiana among accessions of different geographical regions of India using random amplified polymorphic DNA (RAPD) markers and high-performance thin layer chromatography (HPTLC) analysis. The RAPD analysis of Stevia rebaudiana (11 accessions) was carried out using 20 random operon primers. Dendrogram was constructed for cluster analysis based on the unweighted pair group method with arithmetic means (UPGMA) using Winboot. The HPTLC analysis of all samples was carried out on silica using acetone:ethyl acetate:water (5:4:1, v/v/v) for fingerprinting and quantification of stevioside and rebaudioside A at 360 nm after spraying with anisaldehyde sulphuric acid. Ten out of 20 primers screened were found most informative; amplification products of the genotypes yielded a total of 87 scorable bands (67 polymorphic), whereas genetic similarity (GS) coefficient (0.01-0.08) and polymorphism (67.24-92.40%) showed huge variability. Similarly, HPTLC analysis showed large variation among different samples with respect to their presence or absence of metabolite and their concentration. Out of the 11 Stevia accessions, Delhi and Mohali varieties showed much relatedness with each other and were concluded to be the superior genotype in context to RAPD and HPTLC analysis. The information obtained here could be valuable for devising strategies for cultivating this medicinal plant.

  9. A Review on the Pharmacology and Toxicology of Steviol Glycosides Extracted from Stevia rebaudiana.

    Science.gov (United States)

    Momtazi-Borojeni, Amir Abbas; Esmaeili, Seyed-Alireza; Abdollahi, Elham; Sahebkar, Amirhossein

    2017-01-01

    Stevia rebaudiana Bertoni is a sweet and nutrient-rich plant belonging to the Asteraceae family. Stevia leaves contain steviol glycosides including stevioside, rebaudioside (A to F), steviolbioside, and isosteviol, which are responsible for the plant's sweet taste, and have commercial value all over the world as a sugar substitute in foods, beverages and medicines. Among the various steviol glycosides, stevioside, rebaudioside A and rebaudioside C are the major metabolites and these compounds are on average 250-300 times sweeter than sucrose. Steviol is the final product of Stevia metabolism. The metabolized components essentially leave the body and there is no accumulation. Beyond their value as sweeteners, Stevia and its glycosdies possess therapeutic effects against several diseases such as cancer, diabetes mellitus, hypertension, inflammation, cystic fibrosis, obesity and tooth decay. Studies have shown that steviol glycosides found in Stevia are not teratogenic, mutagenic or carcinogenic and cause no acute and subacute toxicity. The present review provides a summary on the biological and pharmacological properties of steviol glycosides that might be relevant for the treatment of human diseases. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  10. [Determination of steviol in Stevia Rebaudiana leaves by near infrared spectroscopy].

    Science.gov (United States)

    Tang, Qi-Kun; Wang, Yul; Wu, Yue-Jin; Min, Di; Chen, Da-Wei; Hu, Tong-Hua

    2014-10-01

    The objective of the present study is to develop a method for rapid determination of the content of stevioside (ST) and rebaudioside A (RA) in Stevia Rebaudiana leaves. One hundred and five samples of stevia from different areas containing ST of 0.27%-1.40% and RA of 0.61%-3.98% were used. The 105 groups' NIRS diagram was processed by different methods including subtracting a straight line (SLS), multiplicative scatter correction (MSC), first derivative (FD), second derivative (SD) and so on, and then all data were analyzed by partial least square (PLS). The study showed that SLS can be used to extracted spectra information thoroughly to analyze the contents of ST, the correlation coefficients of calibration (Re), the root-mean-square errors of calibration (RMSEC) and prediction (RMSEP), and the residual predictive deviation (RPD) were 0.986, 0.341, 1.00 and 2.8, respectively. The correlation coefficients of RA was 0.967, RMSEC was 1.50, RMSEP was 1.98 and RPD was 4.17. The results indicated that near infrared spectroscopy (NIRS) technique offers effective quantitative capability for ST and RA in Stevia Rebaudiana leaves. Then the model of stevia dried leaves was used to compare with the stevia powder near infrared model whose correlation coefficients of ST was 0.986, RMSEC was 0.32, RMSEP was 0.601 and RPD was 2.86 and the correlation coefficients of RA was 0.968, RMSEC was 1.50, RMSEP was 1.48 and RPD was 4.2. The result showed that there was no significant difference between the model of dried leaves and that of the powders. However, the dried leaves NIR model reduces the unnecessary the steps of drying and grinding in the actual detection process, saving the time and reducing the workload.

  11. Effect of nitrogen sources on some morphological characteristics of in vitro stevia rebaudiana Bertoni.

    Science.gov (United States)

    Akbari, F; Arminian, A; Kahrizi, D; Fazeli, A

    2017-02-28

    Stevia rebaudiana Bertoni belongs to Asteraceae family that leaves 200-300 times sweeter than sugar. Low seed fertility is one of the most important problems in Stevia production. So, Plant tissue culture is an efficient method for mass propagation of Stevia. In this research, we studied the effect of various concentrations of nitrogen on some morphological traits of stevia under in vitro conditions. We used axillary nodes as explants and they were cultured on Murashige and Skoog (MS) medium containing inorganic nitrogen sources i.e. NH4NO3(0, 825 and 1650 mg/l), KNO3(0, 950 and 1900 mg/l) were observed. The cultures were kept for 4 weeks at a temperature of 25±2°C with a photoperiod of 16/8 hour low light/dark each day. Maximum shoot length (89.33 mm), dry weight of plants (0.10 mg) and leaf fresh weight (0.42 mg) was observed on MS medium with 1650 mg/l NH4NO3 and 950 mg/l KNO3. Minimum shoot length (6.13 mm), root length (6.60 mm), leaf number (4.26), leaf dry weight (0.01 mg), leaf fresh weight (0.05 mg), total dry and fresh weight (0.02 and 0.15 mg) and growth rate was observed on a MS medium without nitrogen sources. Moreover, presence of nitrogen sources increases both shooting and rooting in Stevia rebaudiana Bertoni.

  12. [Development of a gummy candy reduced in calories by sugar substitution with Stevia rebaudiana B].

    Science.gov (United States)

    Aranda-González, Irma; Tamayo-Dzul, Óscar; Barbosa-Martín, Enrique; Segura-Campos, Maira; Moguel-Ordoñez, Yolanda; Betancur-Ancona, David

    2014-09-12

    The consumption of gummy candy is widespread among people of different ages but mainly by children. The formulation of this product requires sugar that contributes to their flavor and consistency, but with the undesirable effect of increase its glycemic index and its calories from simple sugars; it is known that consumption of products with these last two characteristics are related to childhood obesity, which is a worldwide growing disease. Stevia rebaudiana is a plant that naturally contains glycosides with high sweetening power and it is considered safe for consumption. Therefore the aim of this work was to develop a gummy candy reduced in calories by replacing sugar with Stevia rebaudiana B., and analyzes its texture and acceptability. Gummy candy were prepared with different percentage of sugar reduction (-20, -40, -60, -80 and -100%) and a product control (100% sugar); gummy elasticity was assess by displacement and maximum deformation, whereas resistance was evaluated by breaking strength; those gummies with better elasticity and resistance parameters underwent proximate analysis and sensory evaluations with a unstructured scale applied to 90 school children aged between 6 and 10 years old. A gummy candy reduced in calories with 60% sugar substitution with S. rebaudiana was developed; the level of satisfaction in school children was statistically the same respect to the gummy made of 100% sugar (p <0.05). Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.

  13. Identification of Stevioside Using Tissue Culture-Derived Stevia (Stevia rebaudiana) Leaves

    OpenAIRE

    Karim, Md. Ziaul; Uesugi, Daisuke; Nakayama, Noriyuki; Hossain, M. Monzur; Ishihara, Kohji; Hamada, Hiroki

    2016-01-01

    Stevioside is a natural sweetener from Stevia leaf, which is 300 times sweeter than sugar. It helps to reduce blood sugar levels dramatically and thus can be of benefit to diabetic people. Tissue culture is a very potential modern technology that can be used in large-scale disease-free stevia production throughout the year. We successfully produced stevia plant through in vitro culture for identification of stevioside in this experiment. The present study describes a potential method for iden...

  14. Antidiabetic activity and phytochemical screening of crude extract of Stevia rebaudiana in alloxan-induced diabetic rats.

    Science.gov (United States)

    Kujur, R S; Singh, Vishakha; Ram, Mahendra; Yadava, Harlokesh Narayan; Singh, K K; Kumari, Suruchi; Roy, B K

    2010-07-01

    Stevia rebaudiana regulates blood sugar, prevents hypertension and tooth decay. Other studies have shown that it has antibacterial as well as antiviral property. Preliminary phytochemical screening of aqueous, ether and methanolic extracts of S. rebaudiana was done. Acute and sub-acute toxicity were conducted on twenty four Albino rats, divided into one control (Group I) and three treatment groups viz. aqueous extract (Group II), ether extract (Group III) and methanolic extract (Group IV). For the study of antidiabetic effect of S. rebaudiana rats were divided into seven groups (n=6). Diabetes was induced by a single dose of 5% alloxan monohydrate (125 mg/kg, i.p.) after 24 hour fasting.Blood samples were analysed on day 0, 1, 5, 7, 14 and 28. Phytochemical tests showed presence of different kinds of phyto-constituents in aqueous, ether and methanol extract of Stevia rebaudiana leaves. Daily single dose (2.0 g/kg) administration of aqueous extract (A.E.) , ether extract (E.E.) and methanol extract (M.E.) for 28 days of S. rebaudiana could not show any significant change in ALT and AST levels in rats. Blood sugar level was found to be decreased on day 28 in groups of rats treated with A.E., E.E. and M.E. of S. rebaudiana. The extracts of Stevioside rebaudiana could decrease the blood glucose level in diabetic rats in time dependent manner.

  15. Identification of Stevioside Using Tissue Culture-Derived Stevia (Stevia rebaudiana) Leaves

    Science.gov (United States)

    Karim, Md. Ziaul; Uesugi, Daisuke; Nakayama, Noriyuki; Hossain, M. Monzur; Ishihara, Kohji; Hamada, Hiroki

    2015-01-01

    Stevioside is a natural sweetener from Stevia leaf, which is 300 times sweeter than sugar. It helps to reduce blood sugar levels dramatically and thus can be of benefit to diabetic people. Tissue culture is a very potential modern technology that can be used in large-scale disease-free stevia production throughout the year. We successfully produced stevia plant through in vitro culture for identification of stevioside in this experiment. The present study describes a potential method for identification of stevioside from tissue culture-derived stevia leaf. Stevioside in the sample was identified using HPLC by measuring the retention time. The percentage of stevioside content in the leaf samples was found to be 9.6%. This identification method can be used for commercial production and industrialization of stevia through in vitro culture across the world. PMID:28008268

  16. Stevia rebaudiana Bertoni: A Natural Alternative for Treating Diseases Associated with Metabolic Syndrome.

    Science.gov (United States)

    Carrera-Lanestosa, Areli; Moguel-Ordóñez, Yolanda; Segura-Campos, Maira

    2017-10-01

    Stevia rebaudiana (SR) is often used by the food industry due to its steviol glycoside content, which is a suitable calorie-free sweetener. Further, both in vitro and in vivo studies indicate that these glycosides and the extracts from SR have pharmacological and therapeutic properties, including antioxidant, antimicrobial, antihypertensive, antidiabetic, and anticancer. This work reviews the antiobesity, antihyperglycemic, antihypertensive, and antihyperlipidemic effects of the majority of glycosides and aqueous/alcoholic extracts from the leaves, flowers, and roots of the SR. These compounds can serve as a natural and alternative treatment for diseases that are associated with metabolic syndrome, thus contributing to health promotion.

  17. Estudi agronòmic sobre l'estèvia (Stevia rebaudiana Bertoni)

    OpenAIRE

    Esquerda Serrano, Carles

    2012-01-01

    Aquest projecte fi de carrera consisteix en l’estudi de diversos aspectes agronòmics de la planta de nom botànic Stevia rebaudiana Bertoni, coneguda comunament com estèvia. S’ha estudiat la seva adaptació als hiverns per tal de considerar si l’estèvia pot constituir un cultiu plurianual en les nostres condicions agroclimàtiques. S’han assajat també algunes pràctiques agronòmiques pel que fa a la multiplicació vegetativa de l’estèvia. Finalment, s’ha fet un seguiment de les produccions obting...

  18. Stevia rebaudiana Bert. leaf extracts as a multifunctional source of natural antioxidants.

    Science.gov (United States)

    Gaweł-Bęben, Katarzyna; Bujak, Tomasz; Nizioł-Łukaszewska, Zofia; Antosiewicz, Beata; Jakubczyk, Anna; Karaś, Monika; Rybczyńska, Kamila

    2015-03-27

    The aim of the presented study was to characterize the content and biological activity of extracts prepared from dried Stevia rebaudiana leaves with potential application in the food or cosmetic industry. Aqueous (A), ethanolic (E) and glycol-aqueous (GA) extracts were analyzed for the content of polyphenols and proteins, showing that the highest amount of phenols (15.50 mg/g) and flavonoids (3.85 mg/g) contained GA. All extracts contained significant amount of protein (69.40-374.67 mg/g). Between analyzed stevia extracts (HPLC) GA contained the highest amount of polyphenols, especially ferulic (5.50 mg/g) and rozmaric (4.95 mg/g) acids derivates. The highest antiradical activity against DPPH• and ABTS•+ was noted for GA and E (IC50 = 0.38 and 0.71 µg flavonoids/mL). The highest ability to chelate Fe2+ was observed for E (IC50 = 2.08 µg flavonoids/mL). Stevia extracts were also analyzed for their cytotoxicity and fibroblast irritation potential in vitro. E and GA were the most cytotoxic and irritating, probably due to the high content of biologically active phytochemicals. On the other hand, a extract was the most tolerable by the cells. To summarize, the presented study evaluated the potential application of A, E and GA stevia extracts as natural source of antioxidants in the food and cosmetic industry.

  19. Obtaining a filter irish lace marigold (Tagetes filifolia Lag. sweetened with stevia leaves (Stevia rebaudiana Bertoni

    Directory of Open Access Journals (Sweden)

    Carlos Millones

    2014-03-01

    Full Text Available The present investigation was conducted to determine the segment of the irish lace marigold and appropriate formulation of irish lace marigold and leaves of stevia in the development of a filter sweetened, for which plants were used irish lace marigold and leaves stevia, which were washed, disinfected, dried at 65 °C, ground, sieved and packed; later formulations were made with irish lace marigold and stevia leaves (90:10, 85:15, 80:20 and 75:25, using a gram per sample packed in sealable paper, the results of the color, infusion time and pH, also the organoleptic evaluations smell, taste, sweetness and overall appearance were processed using the SAS statistical package (Statistical Analysis System for Window V8. The results showed that using 80 to 85 % leaves + flowers irish lace marigold and 15 to 20% stevia leaf with a sweetened organoleptically suitable filter is obtained.

  20. Identification, quantification, and sensory characterization of steviol glycosides from differently processed Stevia rebaudiana commercial extracts.

    Science.gov (United States)

    Espinoza, María Inés; Vincken, Jean-Paul; Sanders, Mark; Castro, Cristian; Stieger, Markus; Agosin, Eduardo

    2014-12-10

    Stevia rebaudiana is known for its sweet-tasting ent-kaurene diterpenoid glycosides. Several manufacturing strategies are currently employed to obtain Stevia sweeteners with the lowest possible off-flavors. The chemical composition of four commercial S. rebaudiana extracts, obtained by different technologies, was characterized using UHPLC-ESI-MS(n). The composition of one of the ethanol-crystallized extracts (EC2) was entirely rebaudioside A, whereas the enzymatically modified (EM) extract contained the lowest concentration of this compound (2.7 mg/100 mg). The membrane-purified (MP) extract had the highest content of minor natural steviol glycosides (23.7 mg/100 mg total extract) versus an average of 2.4 mg/100 mg total extract for the EC samples. Thirteen trained panelists evaluated sweetness, bitterness, licorice, and metallic attributes of all four extracts. The highest licorice intensity (p ≤ 0.05) was found for MP. Both samples EC1 and EC2, despite their different chemical compositions, showed no significant differences in sensory perception.

  1. Effect of different drying methods on the composition of steviol glycosides in Stevia rebaudiana Bertoni leaves

    Science.gov (United States)

    Aranda-González, Irma; Betancur-Ancona, David; Chel-Guerrero, Luis; Moguel-Ordóñez, Yolanda

    2017-01-01

    Drying techniques can modify the composition of certain plant compounds. Therefore, the aim of the study was to assess the effect of different drying methods on steviol glycosides in Stevia rebaudiana Bertoni leaves. Four different drying methods were applied to Stevia rebaudiana Bertoni leaves, which were then subjected to aqueous extraction. Radiation or convection drying was performed in stoves at 60°C, whereas shade or sun drying methods were applied at 29.7°C and 70% of relative humidity. Stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, dulcoside A, and steviolbioside were quantified by a validated HPLC method. Among steviol glycosides, the content (g 100 g-1 dry basis) of stevioside, rebaudioside A, rebaudioside B, and rebaudioside C varied according to the drying method. The total glycoside content was higher in sun-dried samples, with no significant differences compared to shade or convection drying, whereas radiation drying adversely affected the content of rebaudioside A and rebaudioside C (p <0.01) and was therefore a method lowering total glycoside content. The effect of the different drying methods was also reflected in the proportion of the sweetener profile. Convection drying could be suitable for modern food processing industries while shadow or sun drying may be a low-cost alternative for farmers.

  2. Green synthesis of gold nanoparticles using Stevia rebaudiana leaf extracts: Characterization and their stability.

    Science.gov (United States)

    Sadeghi, Babak; Mohammadzadeh, M; Babakhani, B

    2015-07-01

    Various methods invented and developed for the synthesis of gold nanoparticles that increases daily consumed. According to this method, including potential environmental pollution problems and the complexity of the synthesis, in this study, the feasibility of using the leaves extract of Stevia rebaudiana (SR) for the reduction of gold ions to nanoparticles form have been studied. Stevia leaves were used to prepare the aqueous extract for this study. Gold nanoparticles were characterized with different techniques such as UV-vis spectroscopy, FT-IR spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Transmission electron microscopy experiments showed that these nanoparticles are spherical and uniformly distributed and its size is from 5 to 20 nm. FT-IR spectroscopy revealed that gold nanoparticles were functionalized with biomolecules that have primary amine group (NH2), carbonyl group, OH groups and other stabilizing functional groups. X-ray diffraction pattern showed high purity and face centered cubic structure of gold nanoparticles with size of 17 nm. The scanning electron microscopy (SEM) implies the right of forming gold nanoparticles. The results, confirm that gold nanoparticles have synthesized by the leaves extract of S. rebaudiana (SR). Copyright © 2015 Elsevier B.V. All rights reserved.

  3. Chemical composition of leaf extracts of Stevia rebaudiana Bertoni grown experimentally in Vojvodina

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    IVANA S. MARKOVIC

    2008-03-01

    Full Text Available The chemical composition of leaf extracts of Stevia rebaudiana Bertoni, grown for the first time on an experimental field near Zrenjanin, was examined by GC–MS. The tested plant material was harvested in September of 2002. To analyze the chemical composition of the lipophilic components of the plant leaves, essential oils and ethyl acetate extract were isolated. Qualitative analysis of the essential oil obtained by hydrodistillation showed that among the identified 88 compounds, the majority were mono- and sesquiterpenes (50 types identified. By analysing the ethyl acetate extract, the presence of fatty acids (present as free and as esters, n-alkanes, n-alkenes, cyclic alkanes, alcohols, aldehydes, ketones, etc. was ascertained. Sesquiterpenes prevailed among the terpenes (50 types identified. Further constituents identified in ethyl acetate extract included sterols. Nerol, b-cyclocitral, safranal, aromadendrene, a-amorphene and T-muurolol were identified for the first time in this species, with match values over 90 %. Taking into consideration that these terpenes were identified for the first time in this species, it is obvious that Stevia rebaudiana grown in this area possesses certain specific characteristics that can be ascribed to cultivation on a domestic plantation.

  4. Extraction of Stevia rebaudiana bertoni sweetener glycosides by supercritical fluid methods.

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    Juan José Hinojosa-González

    2017-05-01

    Full Text Available Aim. The aim was to evaluate the supercritical carbon dioxide extraction method with and without the addition of co-solvent to the system (mixture water: ethanol to obtain the glycosides from leaves of Stevia rebaudiana Bertoni. Methods. A SFT-150 SFE / SFR model with CO2 as a fluid was used for the supercritical extraction. The variables studied were temperature, pressure, extraction time and the presence or absence of the co-solvent (water-ethanol mixture in a concentration of 70:30 v/v, incorporated in different proportions to determine the effect on yield. The amount of glycoside sweeteners was analyzed by High Performance Liquid Chromatography (HPLC. Results. The pressure was the factor that favored the extraction, which was selective in obtaining Rebaudioside A with yields no greater than 2%. The inclusion of the co-solvent achieved an increase in yield to values of 2.9% Conclusion. Supercritical CO2 individually and mixed with ethanol-water as a co-solvent was not efficient to extract Stevia rebaudiana stevioside sweeteners

  5. Crop-ecology and nutritional variability influence growth and secondary metabolites of Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Pal, Probir Kumar; Kumar, Rajender; Guleria, Vipan; Mahajan, Mitali; Prasad, Ramdeen; Pathania, Vijaylata; Gill, Baljinder Singh; Singh, Devinder; Chand, Gopi; Singh, Bikram; Singh, Rakesh Deosharan; Ahuja, Paramvir Singh

    2015-02-27

    Plant nutrition and climatic conditions play important roles on the growth and secondary metabolites of stevia (Stevia rebaudiana Bertoni); however, the nutritional dose is strongly governed by the soil properties and climatic conditions of the growing region. In northern India, the interactive effects of crop ecology and plant nutrition on yield and secondary metabolites of stevia are not yet properly understood. Thus, a field experiment comprising three levels of nitrogen, two levels of phosphorus and three levels of potassium was conducted at three locations to ascertain whether the spatial and nutritional variability would dominate the leaf yield and secondary metabolites profile of stevia. Principal component analysis (PCA) indicates that the applications of 90 kg N, 40 kg P2O5 and 40 kg K2O ha-1 are the best nutritional conditions in terms of dry leaf yield for CSIR-IHBT (Council of Scientific and Industrial Research- Institute Himalayan Bioresource Technology) and RHRS (Regional Horticultural Research Station) conditions. The spatial variability also exerted considerable effect on the leaf yield and stevioside content in leaves. Among the three locations, CSIR-IHBT was found most suitable in case of dry leaf yield and secondary metabolites accumulation in leaves. The results suggest that dry leaf yield and accumulation of stevioside are controlled by the environmental factors and agronomic management; however, the accumulation of rebaudioside-A (Reb-A) is not much influenced by these two factors. Thus, leaf yield and secondary metabolite profiles of stevia can be improved through the selection of appropriate growing locations and proper nutrient management.

  6. In Vitro Callus Induction and Growth of Stevia (Stevia rebaudiana Bertoni M. with Difference Concentrations of PEG (Polyethylene Glycol and Light Conditions

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    Mohamad Ana Syabana

    2017-07-01

    Full Text Available Stevia (Stevia rebaudiana Bert. M. is known as a natural non-caloric sweetener. This plants contain glycoside such steviosida type, mainly on the leave contain sweetness level between 200-300 cane sugar but the calorie is very low. This research was aimed to determine difference effects of PEG (Polyethylene Glycol concentrations and the light conditions on Stevia (Stevia rebaudiana Bert. M. callus induction in vitro. This research was conducted from April to June 2016 at Biotechnology Laboratory Faculty of Agriculture, Sultan Ageng Tirtayasa University. This research used a completely randomized design (CRD, which consisted of two factors with three replications. Concentrations of PEG as first factor consisted of four levels (0 mg/L, 5 mg/L, 15 mg/L, and 25 mg/L. Light conditions as second factor consisted of two levels (Dark and Light. The results showed that the concentrations of PEG (Polyethylene Glycol did not significantly effect the time of callus appearance and diameter of callus on 4, 5 and 6 weeks after planting. Dark condition was the best conditions for callus induction of stevia. The texture of callus was compact on all treatments and the callus dominant color produced is golden brown.

  7. In Vitro Callus Induction and Growth of Stevia (Stevia rebaudiana Bertoni M. with Difference Concentrations of PEG (Polyethylene Glycol and Light Conditions

    Directory of Open Access Journals (Sweden)

    Mohamad Ana Syabana

    2017-06-01

    Full Text Available Stevia (Stevia rebaudiana Bert. M. is known as a natural non-caloric sweetener. This plants contain glycoside such steviosida type, mainly on the leave contain sweetness level between 200-300 cane sugar but the calorie is very low. This research was aimed to determine difference effects of PEG (Polyethylene Glycol concentrations and the light conditions on  Stevia (Stevia rebaudiana Bert. M. callus induction  in vitro. This research was conducted from April to June 2016 at Biotechnology Laboratory Faculty of Agriculture, Sultan Ageng Tirtayasa University. This research used a completely randomized design (CRD, which consisted of two factors with three replications. Concentrations of PEG as first factor consisted of four levels (0 mg/L, 5 mg/L, 15 mg/L, and 25 mg/L. Light conditions as second factor consisted of two levels (Dark and Light. The results showed that the concentrations of PEG (Polyethylene Glycol did not significantly effect the time of callus appearance and diameter of callus on 4, 5 and 6 weeks after planting. Dark condition was the best conditions for callus induction of stevia. The texture of callus was compact on all treatments and the callus dominant color produced is golden brown

  8. Turbo-extraction of glycosides from Stevia rebaudiana using a fractional factorial design

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    Paula M. Martins

    Full Text Available ABSTRACT Stevia rebaudiana (Bertoni Bertoni, Asteraceae, leaf extract has recently called the attention of food industry as a proposal for natural sweetener. The sweet flavor is attributed to the glycosides, in especial stevioside and rebaudioside A, which are the plant main chemical markers. The aim of the work reported here was to optimize the turbo-extraction of stevia leaves using water, ethanol 70% and 90% (w/w as green solvents. A 25-2 factorial design was applied to study the linear effects of the drug size, solvent to drug ratio, temperature, time and also the turbolysis speed on the extraction of glycosides. The glycosides exhaustive extraction showed that ethanol 70% gave better results and was used for turbo-extraction. The stevioside and rebaudioside A contents were quantified by a validated method by high performance liquid chromatographic with photodiode array detector. The contents of stevioside and rebaudioside A in fluid extract increased with the drug size, but decreased at high shearing speeds and solvent to drug ratio, while their yields decreased at higher temperature and were not affected by turbo speed. An increase in solvent to drug ratio reduced significantly the glycosides percent in dried extract. Optimal solution for S. rebaudiana leaves turbo-extraction was determined by desirability functions. The optimal extraction condition corresponded to drug size of 780 µm, solvent to drug ratio of 10, extraction time of 18 min; temperature of 23 ºC and turbo speed of 20,000 rpm, resulting in yields of 4.98% and 2.70%, for stevioside and rebaudioside A, respectively. These yields are comparable to the ones recently published for dynamic maceration, but with the advantage of shorter extraction times. This work demonstrates that turbolysis is promising for S. rebaudiana glycosides extraction and stimulate new research on the purification of these extracts, which may become an interesting source of income for developing

  9. Green extraction of glycosides from Stevia rebaudiana (Bert. with low solvent consumption: A desirability approach

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    Paula M. Martins

    2016-12-01

    Full Text Available The sweet flavor of Stevia rebaudiana (Bert. leaf extract is well known and has raised the interest of huge food companies due to its natural bid. The extraction of their main glycosides stevioside and rebaudioside A is an important step on the preparation of final Stevia granules. The aim of the work reported here was to study and optimize the dynamic maceration of Stevia leaves using water and ethanol as green solvents. For instance, a fractional factorial design was chosen to evaluate the individual effects of the drug powder size, weight ratio of drug to solvent, temperature, agitation, and time on the yield of these glycosides. The glycosides were quantified by high pressure liquid chromatography. An exhaustive extraction by successive maceration steps showed that ethanol 70% was superior to water and ethanol 90% for stevioside and rebaudioside extraction. The liquid extract composition in dry basis and the yield of stevioside and rebaudioside A were significantly affected by the drug to solvent weight ratio, showing that larger volumes of solvent should be used. Furthermore, increasing solvent volume favors the extraction of the stevioside by a twofold factor as compared to rebaudioside A. Among the other factors, only drug powder size affected the yield of rebaudioside A significantly. The optimal solution for S. rebaudiana leaves dynamic extraction estimated by desirability functions methodology led to a condition which allows obtaining extraction yields of 2.31 and 1.24% for stevioside and rebaudioside A and their concentrations in dried extract corresponding to 8.38 and 4.51%, respectively. These high yields were obtained with drug to solvent ratio (1:10, w/w much higher than previous works, thus resulting in a more sustainable and green process.

  10. Effect of aqueous and alcoholic Stevia (Stevia rebaudiana) extracts against Streptococcus mutans and Lactobacillus acidophilus in comparison to chlorhexidine: An in vitro study

    Science.gov (United States)

    Ajagannanavar, Sunil Lingaraj; Shamarao, Supreetha; Battur, Hemant; Tikare, Shreyas; Al-Kheraif, Abdulaziz Abdullah; Al Sayed, Mohammed Sayed Al Esawy

    2014-01-01

    Introduction: Stevia (S. rebaudiana) a herb which has medicinal value and was used in ancient times as a remedy for a great diversity of ailments and sweetener. Leaves of Stevia contain a high concentration of Stevioside and Rebaudioside which are supposed to be sweetening agents. Aim: To compare the efficacy of aqueous and alcoholic S. rebaudiana extract against Streptococcus mutans and Lactobacillus acidophilus in comparison to chlorhexidine. Materials and Methods: In the first part of the study, various concentrations of aqueous and ethanolic Stevia extract were prepared in the laboratory of Pharmacy College. It was then subjected to microbiological assay to determine its zone of inhibition using Agar disk diffusion test and minimum inhibitory concentration (MIC) using serial broth dilution method against Streptococcus mutans and Lactobacillus acidophilus. Chlorhexidine was used as a positive control. One way Analysis of Variance (ANOVA) test was used for multiple group comparisons followed by Tukey post hoc for group wise comparisons. Results: Minimum inhibitory concentration (MIC) of aqueous and ethnolic Stevia extract against Streptococcus mutans and Lactobacillus acidophilus were 25% and 12.5% respectively. Mean zone of inhibition of the aqueous and alcoholic Stevia extracts against Streptococcus mutans at 48 hours were 22.8 mm and 26.7 mm respectively. Mean zone of inhibition of the aqueous and alcoholic Stevia extracts against Lactobacillus acidophilus at 48 hours were 14.4 mm and 15.1 mm respectively. Mean zone of inhibition of the chlorhexidine against Streptococcus mutans and Lactobacillus acidophilus at 48 hours was 20.5 and 13.2 respectively. Conclusion: The inhibitory effect shown by alcoholic Stevia extract against Streptococcus mutans and Lactobacillus acidophilus was superior when compared with that of aqueous form and was inferior when compared with Chlorhexidine. PMID:25558451

  11. Stevia rebaudiana loaded titanium oxide nanomaterials as an antidiabetic agent in rats

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    Ariadna Langle

    Full Text Available Abstract Stevia rebaudiana (Bertoni Bertoni, Asteraceae, is a plant with hypoglycemic and antihyperlipidemic properties. S. rebaudiana (SrB has become a lead candidate for the treatment of the diabetes mellitus. However, chronic administrations of S. rebaudiana are required to cause the normoglycemic effect. Importantly, nanomaterials in general and titanium dioxide (TiO2 in particular have become effective tools for drug delivery. In this work, we obtained TiO2 nanomaterials with SrB at different concentrations (10, 20 and 30 µM by sol–gel method. After this nanomaterials were characterized by Fourier transform infrared spectroscopy and transmission electron microscopy. Where it was demonstrated, the presence of the S. rebaudiana in TiO2 nanomaterials, which were observed as hemispherical agglomerated particles of different sizes. The nanomaterials were evaluated in male rats whose diabetes mellitus-phenotype was induced by alloxan (200 mg/kg, i.p.. The co-administration of TiO2-SrB (20 and 30 µM induced a significant and permanent decrease in the glucose concentration since 4 h, until 30 days post-administration. Likewise, the concentrations of insulin, glycosylated hemoglobin, cholesterol, and triacylglycerides showed a significant recovery to basal levels. The major finding of the study was that the TiO2-SrB (20 and 30 µM has a potent and prolonged activity antidiabetic. TiO2 can be considered like an appropriated vehicle in the continuous freeing of active substances to treat of diabetes mellitus.

  12. Changes in sensory characteristics and their relation with consumers' liking, wanting and sensory satisfaction: Using dietary fibre and lime flavour in Stevia rebaudiana sweetened fruit beverages

    DEFF Research Database (Denmark)

    Mielby, Line H.; Andersen, Barbara Vad; Jensen, Sidsel

    2016-01-01

    the sweetener Stevia rebaudiana and the fibre β-glucan seem like very good solutions, as stevia is the only natural non-nutritive sweetener and β-glucans have been related to various health benefits besides increasing satiety. However, both also have distinctive perceptual effects on the sensory characteristics...... of the fruit drinks were affected by stevia and the addition of β-glucans. However, the addition of lime flavour was able to mask the side effect of the aftertaste caused by S. rebaudiana. Further, by adding lime flavour to the fruit beverages, the side effects of increased fibre concentration "Unfresh odour...

  13. Anticariogenic properties and effects on periodontal structures of Stevia rebaudiana Bertoni. Narrative review

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    Soledad Contreras

    2013-12-01

    Full Text Available Introduction: Stevia rebaudiana Bertoni is a natural non-caloric sweetener, with more sweetness than sucrose, without adverse effects, which has demonstrated to have multiples benefits to the systemic health and recently to the oral health. This review’s objective is to describe anti-cariogenic and anti-periodontophatics properties of its extracts. Results: Stevioside and rebaudioside A are the most important glycosides of the Stevia and none is cariogenic. In vitro researches have shown that Stevia extracts have anti-bacterial activity on Streptococcus mutans, Streptococcus sobrinus and Lactobacillus acidophilus, organisms that are closely related to the production and development of tooth decay. In vivo and in vitro it has been observed that the production of bacterial acids decrease attributing it a low acidogenic potential and a lesser effect of the demineralization of the enamel in comparison with others sweeteners. Furthermore, in vivo it has been proved an anti-plaque effect mainly due to a decrease in the production of bacterial insoluble polymers. These characteristics in combination with anti-inflammatory properties could result potentially effective in the treatment of periodontal diseases in significant numbers, as it has been observed in studies conducted in animals. Conclusion: Stevia presents properties that potentially are anti-caries and anti periodontal-diseases. However, in vivo studies are necessary to confirm these assumptions and provide a greater understanding of the mechanisms of action of this plant and the components involved. Notwithstanding, with the existing background, this sweetener can be postulated as a potential therapeutic complement in the odontological care, especially in patients that present base conditions such as obesity, diabetes and high blood pressure.

  14. Structures of the novel α-glucosyl linked diterpene glycosides from Stevia rebaudiana.

    Science.gov (United States)

    Chaturvedula, Venkata Sai Prakash; Upreti, Mani; Prakash, Indra

    2011-09-27

    From the commercial extract of the leaves of Stevia rebaudiana, two new minor diterpene glycosides having α-glucosyl linkage were isolated besides the known steviol glycosides including stevioside, steviolbioside, rebaudiosides A-F, rubusoside and dulcoside A. The structures of the two compounds were identified as 13-[(2-O-(3-α-O-d-glucopyranosyl)-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid β-d-glucopyranosyl ester (1), and 13-[(2-O-β-d-glucopyranosyl-3-O-(4-O-α-d-glucopyranosyl)-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid β-d-glucopyranosyl ester (2), on the basis of extensive NMR and MS spectral data as well as chemical studies. Copyright © 2011 Elsevier Ltd. All rights reserved.

  15. Stimulation of steviol glycoside accumulation in Stevia rebaudiana by red LED light.

    Science.gov (United States)

    Ceunen, Stijn; Werbrouck, Stefaan; Geuns, Jan M C

    2012-05-01

    The aim of this study was to determine whether steviol glycoside accumulation is under phytochrome control. The results indicate that Stevia rebaudiana Bertoni plants grown under short-day conditions showed precocious flowering and stagnation of steviol glycoside accumulation. Long night interruption by red LED light stimulated and sustained the vegetative growth as well as the accumulation of steviol glycosides in the leaves. After 7 weeks of treatment, steviol glycoside content was about two-fold higher in LED-treated plants than in the short-day control group. The effects of red LED light were measured both in a greenhouse and in a phytotron, irrespective of cultivar-specific differences. Therefore, it can be concluded that a mid-night interruption by red LED light during short photoperiods provides an easy and inexpensive method to increase vegetative leaf biomass production with an increased steviol glycoside yield. Copyright © 2012 Elsevier GmbH. All rights reserved.

  16. Separation and purification of rebaudioside A from extract of Stevia Rebaudiana leaves by macroporous adsorption resins

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    Anvari Masoumeh

    2016-03-01

    Full Text Available The separation and purification of rebaudioside A from Stevia rebaudiana crude extracts (Steviosides by macroporous resin were optimized by Taguchi orthogonal array (OA experimental design methodology. This approach was applied to evaluate the influence of five factors (adsorption temperature, desorption time, elution solution ratio, adsorption volume and type of resin on the rebaudioside A yield. The percentage contribution of each factor was also determined. The results showed that elution solution ratio and adsorption volume made the greatest (59.6% and the lowest (1.3% contribution, respectively. The results showed that the Taguchi method is able to model the purification of rebaudioside A process well (R2 > 0.998 and can therefore be applied in future studies conducted in various fields. Adsorption temperature 35°C, desorption time 60min, elution solution ratio 3, adsorption volume 200ml and HPD-400 as resin were the best conditions determined by the Taguchi method.

  17. Glucosylation of Steviol and Steviol-Glucosides in Extracts from Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Shibata, H; Sonoke, S; Ochiai, H; Nishihashi, H; Yamada, M

    1991-01-01

    To evaluate and characterize stevioside biosynthetic pathway in Stevia rebaudiana Bertoni cv Houten, two enzyme fractions that catalyze glucosylation of steviol (ent-13-hydroxy kaur-16-en-19-oic acid) and steviol-glucosides (steviol-13-O-glucopyranoside, steviolbioside and stevioside), utilizing UDP-glucose as the glucose donor, were prepared from the soluble extracts of S. rebaudiana leaves. Enzyme fraction I, passed through DEAE-Toyopearl equilibrated with 50 millimolar K-phosphate pH 7.5, catalyzed the glucosylation to steviol and 19-O-methylsteviol, but not to iso-steviol and 13-O-methylsteviol, indicating that 13-hydroxyl group of the steviol skeleton is glucosylated first from UDP-glucose to produce steviol-13-O-glucopyranoside. Enzyme fraction II, eluted from the DEAE-Toyopearl column with 0.15 molar KCI, catalyzed the glucose transfer from UDP-glucose to steviol-13-O-glucopyranoside, steviolbioside and stevioside, but not to rubusoside (13, 19-di-O-glucopyranoside) and rebaudioside A. The reaction products glucosylated from steviol-13-O-glucopyranoside, steviolbioside and stevioside were identified to be steviolbioside, stevioside and rebaudioside A, respectively. These results indicate that in the steviol-glucoside biosynthetic pathway, steviol-13-O-glucopyranoside produced from the steviol glucosylation is successively glucosylated to steviolbioside, then to stevioside producing rebaudioside A.

  18. Nitrogen Limitation Alters Biomass Production but Enhances Steviol Glycoside Concentration in Stevia rebaudiana Bertoni

    Science.gov (United States)

    Barbet-Massin, Claire; Giuliano, Simon; Alletto, Lionel; Daydé, Jean; Berger, Monique

    2015-01-01

    The need for medicinal and aromatic plants for industrial uses creates an opportunity for farmers to produce alternative crops. Stevia rebaudiana Bertoni, a perennial shrub originating from Paraguay, is of increasing interest as a source of zero-calorie natural sweeteners: the steviol glycosides (SVglys). The aim of this study was to investigate the relevance of nitrogen (N) supply for leaf yield and for SVgly concentrations in leaves, which are the two major components of S. rebaudiana productivity. In this regard, the relationship between leaf N concentration, CO2 assimilation, leaf production and SVgly accumulation was investigated. The experiments were conducted consecutively in growth-chamber (CC: controlled conditions), in greenhouse (SCC: semi-controlled conditions) and in field conditions (FC) on two genotypes. In CC and SCC, three levels of N fertilization were applied. Plants were grown on four locations in the FC experiment. Both N supply (CC and SCC) and location (FC) had a significant effect on N content in leaves. When light was not limiting (SCC and FC) N content in leaves was positively correlated with CO2 assimilation rate and biomass accumulation. Irrespective of the growth conditions, N content in leaves was negatively correlated with SVgly content. However, increased SVgly content was correlated with a decreased ratio of rebaudioside A over stevioside. The evidence that the increased SVgly accumulation compensates for the negative effect on biomass production suggests that adequate SVgly productivity per plant may be achieved with relatively low fertilization. PMID:26192921

  19. Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana.

    Science.gov (United States)

    Fu, Xiao; Yin, Zhong-Ping; Chen, Ji-Guang; Shangguan, Xin-Chen; Wang, Xiaoqiang; Zhang, Qing-Feng; Peng, Da-Yong

    2015-01-14

    Chlorogenic acid and its derivatives (CADs) are valuable bioactive plant secondary metabolites with many health benefits. In the present study, Stevia rebaudiana hairy root cultures were established, and the culture conditions for the production of CADs were optimized. The hairy roots were induced by coculture of S. rebaudiana leaves and Agrobacterium rhizogenes (C58C1) after infection, which were further verified by PCR detection of rolB and rolC genes. HPLC-MS and HPLC analysis showed that chlorogenic acid (3-caffeoylquinic acid, 3-CQA), 3,5-dicaffeoylquinic acid (3,5-CQA), and 4,5-dicaffeoylquinic acid (4,5-CQA) were the major CADs in the hairy roots. Eight single roots with rapid growth rate were selected. Among them, T3 had the highest yield of CADs. B5 medium supplemented with 40 g/L sucrose was more suitable for the production of CADs than others. Under optimal culture conditions, the total content of these three compounds reached 105.58 mg/g and total yield was 234.40 mg/100 mL.

  20. Nitrogen Limitation Alters Biomass Production but Enhances Steviol Glycoside Concentration in Stevia rebaudiana Bertoni.

    Directory of Open Access Journals (Sweden)

    Claire Barbet-Massin

    Full Text Available The need for medicinal and aromatic plants for industrial uses creates an opportunity for farmers to produce alternative crops. Stevia rebaudiana Bertoni, a perennial shrub originating from Paraguay, is of increasing interest as a source of zero-calorie natural sweeteners: the steviol glycosides (SVglys. The aim of this study was to investigate the relevance of nitrogen (N supply for leaf yield and for SVgly concentrations in leaves, which are the two major components of S. rebaudiana productivity. In this regard, the relationship between leaf N concentration, CO2 assimilation, leaf production and SVgly accumulation was investigated. The experiments were conducted consecutively in growth-chamber (CC: controlled conditions, in greenhouse (SCC: semi-controlled conditions and in field conditions (FC on two genotypes. In CC and SCC, three levels of N fertilization were applied. Plants were grown on four locations in the FC experiment. Both N supply (CC and SCC and location (FC had a significant effect on N content in leaves. When light was not limiting (SCC and FC N content in leaves was positively correlated with CO2 assimilation rate and biomass accumulation. Irrespective of the growth conditions, N content in leaves was negatively correlated with SVgly content. However, increased SVgly content was correlated with a decreased ratio of rebaudioside A over stevioside. The evidence that the increased SVgly accumulation compensates for the negative effect on biomass production suggests that adequate SVgly productivity per plant may be achieved with relatively low fertilization.

  1. [Safety assessment of stevia rebaudiana bertoni grown in southeastern Mexico as food sweetener].

    Science.gov (United States)

    Aranda-González, Irma; Barbosa-Martín, Enrique; Toraya-Avilés, Rocío; Segura-Campos, Maira; Moguel-Ordoñez, Yolanda; Betancur-Ancona, David

    2014-09-01

    Stevia rebaudiana leaves and their glycosides have been recently and significantly used so important as sweeteners. However, it has been reported an antihyperglycemic effect of the extract and a glycoside. The aim of this study was to quantify S. rebaudiana glycosides, assess cytotoxicity of the extract and its acute and chronic effect on blood glucose in animal models and in human. The glycosides of the Morita II and Criolla extract were quantified by HPLC, using a C18 column (250 mm x 4.6 mm and particle size of 5 uM) with UV detection at 210 nm, mobile phase of acetonitrile/sodium phosphate buffer 10 mmol/L, pH 2.6 (32:68 v/v). Cytotoxicity study was performed in Vero cells, whereas an intraperitoneal glucose tolerance test (IPGTT) and a chronic consumption assay (4 weeks) were executed in an animal model of diabetes; finally the glycemic index (G.I.) was determined in healthy individuals. The glycoside content is higher in the Morita variety II although both had a CC50 >300 g/mL. The areas under the curve of the IPGTT and fasting glucose of the animals were not significantly different (p> 0.05) and the I.G. extract was 11.11 %, which classifies the extract as low I.G. The extract of S. rebaudiana Morita II has a low glycemic index and, in the doses tested, is not cytotoxic nor has acute or chronic effect on blood sugar, which makes it a safe sweetener. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.

  2. Antioxidant capacity of leaf extracts from two Stevia rebaudiana Bertoni varieties adapted to cultivation in Mexico.

    Science.gov (United States)

    Ruiz Ruiz, Jorge Carlos; Moguel Ordoñez, Yolanda Beatriz; Matus Basto, Ángel; Segura Campos, Maira Rubi

    2014-09-12

    The recent introduction of the cultivation of Stevia rebaudiana Bertoni in Mexico has gained interest for its potential use as a non-caloric sweetener, but some other properties of this plant require studies. Extracts from two varieties of S. rebaudiana Bertoni adapted to cultivation in Mexico were screened for their content of some phytochemicals and antioxidant properties. Total pigments, total phenolic and flavonoids contents of the extracts ranged between 17.7-24.3 mg/g, 28.7-28.4 mg/g, and 39.3-36.7 mg/g, respectively. The variety "Criolla" exhibited higher contents of pigments and flavonoids. Trolox equivalent antioxidant capacity ranged between 618.5-623.7 mM/mg and DPPH decolorization assay ranged between 86.4-84.3%, no significant differences were observed between varieties. Inhibition of β-carotene bleaching ranged between 62.3-77.9%, with higher activity in the variety "Criolla". Reducing power ranged between 85.2-86% and the chelating activity ranged between 57.3-59.4% for Cu²⁺ and between 52.2-54.4% for Fe²⁺, no significant differences were observed between varieties. In conclusion, the results of this study showed that polar compounds obtained during the extraction like chlorophylls, carotenoids, phenolic compounds and flavonoids contribute to the antioxidative activity measured. The leaves of S. rebaudiana Bertoni could be used not only as a source of non-caloric sweeteners but also naturally occurring antioxidants. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.

  3. Antioxidant response of Stevia rebaudiana B. to polyethylene glycol and paclobutrazol treatments under in vitro culture.

    Science.gov (United States)

    Hajihashemi, Shokoofeh; Ehsanpour, Ali Akbar

    2014-04-01

    This investigation was carried out with the aim of determining the effect of paclobutrazol (PBZ) (0 and 2 mg l(-1)) and polyethylene glycol (PEG) (0, 2, 4 and 6 % w/v of PEG 6000) treatments on antioxidant system of Stevia rebaudiana Bertoni under in vitro condition. Analysis of data showed that PEG treatment significantly increased hydrogen peroxide (H2O2) and phenolic contents, while PBZ treatment limited the effect of PEG on them. Our data revealed that PEG treatment significantly increased total antioxidant capacity, catalase (CAT), ascorbate peroxidase (APX), polyphenol oxidase (PPO) and peroxidase (POD) activity, while it inversely decreased glutathione reductase (GR) activity. The superoxide dismutase (SOD) activity was not affected by PEG treatment. PBZ treatment induced significantly higher levels of CAT and GR activity and lower levels of SOD activity in PEG-treated plants. PBZ in combination with PEG resulted in no significant difference on APX activity with PEG treatment alone. PBZ treatment prevented the effect of PEG on the PPO activity. PEG (with or without PBZ) treatment increased the ascorbate pool, whereas total glutathione level was not affected by PEG. Our finding indicated that PBZ reduced the negative effect of PEG treatment by quenching H2O2 accumulation and increasing the CAT activity. Collectively, the antioxidant capacity of S. rebaudiana in PEG treatment condition was associated with active enzymatic and non-enzymatic defence systems which partly could be improved by the PBZ treatment. In addition, a higher accumulation of phenolic compounds leads to a more potent reactive oxygen species scavenging activity in S. rebaudiana.

  4. Clonal propagation and cryogenic storage of the medicinal plant Stevia rebaudiana

    Energy Technology Data Exchange (ETDEWEB)

    Shatnawi, M. A.; Shibli, R. A.; Abu-Romman, S. M.; Al-Mazra awi, M. S.; Al Ajlouni, Z. I.; Shatanawi, W. A.; Odeh, W. H.

    2011-07-01

    Successful clonal propagation of Stevia rebaudiana was achieved using micro shoots as a primary step for in vitro conservation. Maximum proliferation was obtained on Murashige and Skoog (MS) medium supplemented with 1.5 mg L{sup -}1 benzyl amino purine and 0.2 mg L{sup -}1 indole-3-butyric-acid (IBA). Auxin increased rooting percentage of shoots at concentration of 0.4 mg L{sup -}1 IBA, indole-3-acetic-acid or naphthalene acetic acid and no rooting occurred without plant growth regulator. A survival of 90% was achieved when rooted explants were acclimatized in vivo in 1 soil: 1 perlite: 1 peat. In vitro S. rebaudiana shoots were successfully stored for up to 32 weeks on MS medium supplemented with an appropriate concentration of sucrose, sorbitol or mannitol, at 24 {+-} 2 degree centigrade. After 32 weeks, 93.6% of the shoots were able to survive. Moreover, 89.3% of them were able to regrow when stored under light conditions. Cryo preservation by vitrification was successfully achieved (65.6% regrowth) when shoot tips were pre cultured on a medium supplemented with 0.4 M sorbitol for 2 d, followed by loading shoot tips with 80% concentrated plant vitrification solution 2 (PVS2) for 20 min; then dehydrated with 100% PVS2 for 60 min at 0 degree centigrade prior to storage in liquid nitrogen. This procedure is easy to handle and produced a high levels of shoot formation. This protocol could be useful for long term storage of S. rebaudiana germplasm. (Author) 30 refs.

  5. Aspectos de la biología reproductiva de Stevia rebaudiana (Asteraceae

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    Irene Caponio

    2016-01-01

    Full Text Available El género SteviaCav. (Asteraceae presenta alrededor de 200 especies en el continente americano. Stevia rebaudiana (Bertoni Bertoni, originaria de Paraguay, es la única especie cultivada. Ha sido ampliamente estudiada desde el punto de vista químico, por los principios activos que poseen sus hojas. Sin embargo existe controversia respecto a su sistema reproductivo, ya que es considerada apomíctica obligada por algunos autores, mientras otros la consideran de reproducción sexual. A los efectos de clarificar esta cuestión se realizaron estudios de: megasporogénesis, megagametogénesis, fenología de la floración, viabilidad de polen y receptividad estigmática en tres ecotipos en condiciones de autopolinización y polinización cruzada. De acuerdo a los resultados obtenidos, las plantas analizadas se reproducen sexualmente y poseen un sistema de autoincompatibilidad que impide la autofertilización.

  6. Chemical characterization and prebiotic activity of fructo-oligosaccharides from Stevia rebaudiana (Bertoni) roots and in vitro adventitious root cultures.

    Science.gov (United States)

    Sanches Lopes, Sheila Mara; Francisco, Mariane Grigio; Higashi, Bruna; de Almeida, Rafaela Takako Ribeiro; Krausová, Gabriela; Pilau, Eduardo Jorge; Gonçalves, José Eduardo; Gonçalves, Regina Aparecida Correia; Oliveira, Arildo José Braz de

    2016-11-05

    Stevia rebaudiana (Bertoni) is widely studied because of its foliar steviol glycosides. Fructan-type polysaccharides were recently isolated from its roots. Fructans are reserve carbohydrates that have important positive health effects and technological applications in the food industry. The objective of the present study was to isolate and characterize fructo-oligosaccharides (FOSs) from S. rebaudiana roots and in vitro adventitious root cultures and evaluate the potential prebiotic effect of these molecules. The in vitro adventitious root cultures were obtained using a roller bottle system. Chemical analyses (gas chromatography-mass spectrometry, (1)H nuclear magnetic resonance, and off-line electrospray ionization-mass spectrometry) revealed similar chemical properties of FOSs that were obtained from the different sources. The potential prebiotic effects of FOSs that were isolated from S. rebaudiana roots enhanced the growth of both bifidobacteria and lactobacilli, with strains specificity in their fermentation ability. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. A new natural source for obtainment of inulin and fructo-oligosaccharides from industrial waste of Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Lopes, Sheila Mara Sanches; Krausová, Gabriela; Carneiro, José Walter Pedroza; Gonçalves, José Eduardo; Gonçalves, Regina Aparecida Correia; de Oliveira, Arildo José Braz

    2017-06-15

    Fructan-type inulin and fructo-oligosaccharides (FOS) are reserve polysaccharides that offer an interesting combination of nutritional and technological properties for food industry. Stevia rebaudiana is used commercially in the sweetener industry due to the high content of steviol glycosides in its leaves. With the proposal of using industrial waste, the objective of the present study was to isolate, characterize and evaluate the prebiotic activity of inulin and FOS from S. rebaudiana stems. The chemical characterization of the samples by GC-MS, NMR and off-line ESI-MS showed that it was possible to obtain inulin molecules from the S. rebaudiana stems with a degree of polymerization (DP) of 12, and FOS with a DPinulin molecules with higher DP. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Clarification of Stevia rebaudiana (Bert. Bertoni extract by adsorption in modified zeolites Clarificação de extrato de Stevia rebaudiana (Bert. Bertoni por adsorção em zeólitas modificadas

    Directory of Open Access Journals (Sweden)

    Elida de Paula Moraes

    2001-05-01

    Full Text Available Selective adsorption on zeolites X and A was studied with Stevia rebaudiana (Bert. Bertoni (Asteraceae extract clarification. Ionic exchanges were made with calcium and barium ions in zeolites NaX and NaA and the effect of contact of aqueous extract of dry Stevia rebaudiana Bertoni leaves with CaX, BaX, CaA and BaA zeolites was evaluated. Stevia extract in contact with the zeolite CaX showed highest clarification and did not present alterations of initial characteristics in concentration, glycosides and flavor. In batch tests at room temperature, the contact of Stevia aqueous extract and CaX zeolite provided 70-80% clarification. Since zeolite may be reused, an approximate clarification of 65-70% is possible in regenerated zeolite.No presente trabalho, foi estudada a adsorção seletiva do extrato de Stevia rebaudiana (Bert. Bertoni (Asteraceae nas zeólitas X e A. Foram feitas trocas com íons de cálcio e bário nas zeólitas NaX e NaA. Foi avaliado o efeito do contato do extrato aquoso de Stevia com as zeólitas CaX, BaX, CaA and BaA. Os resultados mostraram que o extrato que ficou em contato com a zeólita CaX foi o que obteve maior descoloração e não teve as características iniciais alteradas, em termos de concentração, glicosídeos e sabor. Com testes feitos em batelada à temperatura ambiente, o contato entre o extrato aquoso de Stevia e a zeólita CaX, proporcionou uma descoloração entre 70-80%. Os testes mostraram ser possível a regeneração da zeólita, sendo possível a utilização da zeólita por mais de uma vez no processo, sendo alcançada uma descoloração entre 65-70%.

  9. Large-scale profiling of diterpenoid glycosides from Stevia rebaudiana using ultrahigh performance liquid chromatography/tandem mass spectrometry.

    Science.gov (United States)

    Shafii, Behnaz; Vismeh, Ramin; Beaudry, Randy; Warner, Ryan; Jones, A Daniel

    2012-07-01

    The plant Stevia rebaudiana accumulates a suite of diterpenoid metabolites that are natural sweeteners finding increased use as sugar substitutes. To guide breeding of stevia plants that accumulate substances with desirable flavor in high yield, rapid and accurate methods are needed to profile these substances in plant populations. This report describes an 8-min ultrahigh performance liquid chromatography-tandem mass spectrometry method for separation and quantification of seven stevia glycosides including steviolbioside; stevioside; rebaudiosides A, B, and C; rubusoside; and dulcoside as well as aglycones steviol and isosteviol. This negative mode electrospray ionization/multiple reaction monitoring method yielded low limits of detection stevia glycosides. Stevioside and Reb A, B, and C were quantified in more than 1,100 extracts from stevia leaves as part of a large-scale profiling exercise. Leaf tissue levels in this population spanned about two orders of magnitude for stevioside (2-125 mg/g dry weight), Reb A (2.5-164 mg/g), Reb B (0.5-50 mg/g), and Reb C (1.5-125 mg/g), but levels of individual metabolites exhibited independent variation. The wide spread of metabolite levels highlights the utility and importance of performing targeted metabolic profiling for large plant populations.

  10. The effect of Piriformospora indica inoculation on salt and drought stress tolerance in Stevia rebaudiana under in vitro conditions

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    Fahimeh Seraj

    2016-09-01

    Full Text Available In order to investigate the effect of Piriformospora indica under salt and drought stresses on some vegetative characteristics and physiological parameters of stevia (Stevia rebaudiana Bertoni medicinal plant, an experiment was conducted in factorial arrangement based on completely randomized design with three replicates at Genetics and Agricultural Biotechnology Institute in Sari Agricultural Sciences and Natural Resources University. Factors include three levels of osmatic potential (0, -5, and -10 bar and with three osmotic sources including NaCl (Na, Mannitol (M and NaCl+Mannitol (N+M and inoculation of mycorrhizae like fungi at two levels (non-inoculated and inoculation with fungi. The plantlets were treated for 30 days and then some morphological and physiological parameters were measured. Results of ANOVA showed that there was a significant interaction between osmatic source and levels with fungi inoculation for the most determined parameters. Inoculation of stevia plantlets with P. indica at osmatic level of -5 bar caused either by M or M+Na markedly improved dry weight of leaf (112 and 156%, respectively and aerial parts (49 and 144%, respectively as compared to the uninoculated control. Fungi inoculation positively improved vegetative parameters of stevia plant under most osmatic levels and sources. The most ameliorate effect, however, was observed where M as drought stress or M+Na were adjusted to -5 bar. Therefore, the results of this study represented a positive effect of P. indica inoculation in inproving osmotic tolerance of stevia medicinal plant.

  11. Metabolic engineering of the Stevia rebaudiana ent-kaurene biosynthetic pathway in recombinant Escherichia coli.

    Science.gov (United States)

    Kong, Min Kyung; Kang, Hyun-Jun; Kim, Jin Ho; Oh, Soon Hwan; Lee, Pyung Cheon

    2015-11-20

    The ent-kaurene is a dedicated precursor pool and is responsible for synthesizing natural sweeteners such as steviol glycosides. In this study, to produce ent-kaurene in Escherichia coli, we modularly constructed and expressed two ent-kaurene genes encoding ent-copalyl diphosphate synthase (CPPS) and ent-kaurene synthase (KS) from Stevia rebaudiana known as a typical plant producing steviol glycoside. The CPPS and KS from S. rebaudiana were functionally expressed in a heterologous host E. coli. Furthermore, in order to enhance ent-kaurene production in E. coli, six geranylgeranyl diphosphate synthases (GGPPS) from various microorganisms and eight strains of E. coli as host were compared by measuring ent-kaurene production. The highest ent-kaurene production of approximately 41.1mg/L was demonstrated in E. coli strain MG1655 co-expressing synthetic CPPS-KS module and GGPPS from Rhodobacter sphaeroides. The ent-kaurene production was further increased up to 179.6 mg/L by overexpression of the three key enzymes for isoprenoid precursor, 1-deoxyxylulose-5-phosphate synthase (DXS), farnesyl diphosphate synthase (IspA) and isopentenyl diphosphate isomerase (IDI) from E. coli. Finally, the highest titer of ent-kaurene (578 mg/L) with a specific yield of ent-kaurene of 143.5mg/g dry cell weight was obtained by culturing E. coli strain MG1655 co-expressing the ent-kaurene module, DXS, IDI and IspA in 1L bioreactor containing 20 g/L glycerol. Copyright © 2015 Elsevier B.V. All rights reserved.

  12. EFFECT OF GENOTYPE ON THE in vitro REGENERATION OF Stevia rebaudiana VIA SOMATIC EMBRYOGENESIS

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    Esther Julia NARANJO

    2016-01-01

    Full Text Available Stevia rebaudiana (Asteraceae, es una planta de gran importancia económica debido a sus propiedades medicinales y a la presencia de compuestos endulzantes en sus hojas, los cuales pueden sustituir la sacarosa en gran variedad de productos utilizados por personas con problemas de diabetes y obesidad. Con el propósito de estandarizar un método de propagación eficiente y efectivo para diferentes genotipos de Stevia cultivados en Colombia, en la presente investigación se evaluó el efecto sobre la inducción y desarrollo de embriones somáticos de diferentes combinaciones de los reguladores de crecimiento vegetal 2,4-D, IAA, IBA, 2iP y Zeatina, además de los suplementos adenina y agua de coco en el medio de cultivo basal Murashige y Skoog (1962, adicionado con glutamina. Con la combinación 2,4-D (18.09 μM y 2iP (7.38 μM se obtuvo el mayor número embriones somáticos por explante con características bien definidas. El genotipo tuvo un efecto significativo en la repuesta embriogénica, en el genotipo “SRQ-93” se logró la formación y el desarrollo de embriones somáticos, mientras que en los genotipos “Bertoni” y “Morita II”, solo se obtuvo callo embriogénico y no embriogénico respectivamente. La conversión a plántulas se alcanzó en el medio de regeneración conteniendo GA3 (0.29 μM y carbón activado.

  13. Salt-tolerance mechanisms induced in Stevia rebaudiana Bertoni: Effects on mineral nutrition, antioxidative metabolism and steviol glycoside content.

    Science.gov (United States)

    Cantabella, Daniel; Piqueras, Abel; Acosta-Motos, José Ramón; Bernal-Vicente, Agustina; Hernández, José A; Díaz-Vivancos, Pedro

    2017-06-01

    In order to cope with challenges linked to climate change such as salinity, plants must develop a wide spectrum of physiological and molecular mechanisms to rapidly adapt. Stevia rebaudiana Bertoni plants are a case in point. According to our findings, salt stress has no significant effect on plant growth in these plants, which accumulate sodium (Na + ) in their roots, thus avoiding excessive Na + accumulation in leaves. Furthermore, salt stress (NaCl stress) increases the potassium (K + ), calcium (Ca 2+ ), chloride ion (Cl - ) and proline concentrations in Stevia leaves, which could contribute to osmotic adjustment. We also found that long-term NaCl stress does not produce changes in chlorophyll concentrations in Stevia leaves, reflecting a mechanism to protect the photosynthesis process. Interestingly, an increase in chlorophyll b (Chlb) content occured in the oldest plants studied. In addition, we found that NaCl induced reactive oxygen species (ROS) accumulation in Stevia leaves and that this accumulation was more evident in the presence of 5 g/L NaCl, the highest concentration used in the study. Nevertheless, Stevia plants are able to induce (16 d) or maintain (25 d) antioxidant enzymes to cope with NaCl-induced oxidative stress. Low salt levels did not affect steviolbioside and rebaudioside A contents. Our results suggest that Stevia plants induce tolerance mechanisms in order to minimize the deleterious effects of salt stress. We can thus conclude that saline waters can be used to grow Stevia plants and for Steviol glycosides (SGs) production. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  14. Stevia rebaudiana ethanolic extract exerts better antioxidant properties and antiproliferative effects in tumour cells than its diterpene glycoside stevioside.

    Science.gov (United States)

    López, Víctor; Pérez, Sergio; Vinuesa, Arturo; Zorzetto, Christian; Abian, Olga

    2016-04-01

    Steviol glycosides are currently being used as natural sweeteners by the food industry and Stevia rebaudiana has long been used as a sweet plant in South America for patients suffering from diabetes. In this study, a Stevia rebaudiana ethanolic extract (SREE) was prepared, analysed and tested for antioxidant activity in terms of free radical scavenging properties and antiproliferative effects in cervix (HeLa), pancreatic (MiaPaCa-2) and colonic (HCT116) cancer cells. The antiproliferative mechanism was confirmed by testing the effects on cyclin D1-CDK4. Bioassays were also performed for the diterpene glycoside stevioside. Our results demonstrate that the extract acts as an antioxidant being able to scavenge free radicals, but this activity was not due to stevioside. The extract also induced cell death in the three cell lines, being more active against cervix cancer cells (HeLa); however, the concentration of stevioside needed to produce antiproliferative effects was higher than the amount of steviol glycosides found in a lower dose of extract inducing cell death. In addition, the extract clearly inhibited CDK4 whereas stevioside did not, concluding that the antiproliferative activity of stevia may be due to inhibition of cyclin-dependent kinases performed by other compounds of the extract.

  15. Sweeteners from plants--with emphasis on Stevia rebaudiana (Bertoni) and Siraitia grosvenorii (Swingle).

    Science.gov (United States)

    Pawar, Rahul S; Krynitsky, Alexander J; Rader, Jeanne I

    2013-05-01

    In addition to their widely recognized use as dietary supplement ingredients, plant-derived compounds are increasingly used as natural sweeteners. The search for nonnutritive sweeteners has been stimulated over the last 20-30 years by concern over demonstrated or suspected relationships between consumption of sucrose and high-fructose corn syrups and a variety of health-related conditions. In the USA, there is increased use of plant extracts known to contain highly sweet terpenoids. Purified extracts of Stevia rebaudiana (Bertoni) containing the diterpene glycosides stevioside and rebaudioside A are popular as sweeteners and are also used as dietary supplements, and soft drinks and nutritional and energy shakes incorporating extracts of Siraitia grosvenorii (Swingle) fruits containing sweet triterpene glycosides such as mogroside V are also on the market. Here, we review recent studies on these two important sources of noncaloric natural sweeteners, including analytical methods used to identify and quantify specific constituents and structural features relating to their sweetness. We also review the generally recognized as safe status of specific components and their status with respect to review by the Joint FAO/WHO Expert Committee on Food Additives.

  16. Glycosides from Stevia rebaudiana Bertoni Possess Insulin-Mimetic and Antioxidant Activities in Rat Cardiac Fibroblasts.

    Science.gov (United States)

    Prata, Cecilia; Zambonin, Laura; Rizzo, Benedetta; Maraldi, Tullia; Angeloni, Cristina; Vieceli Dalla Sega, Francesco; Fiorentini, Diana; Hrelia, Silvana

    2017-01-01

    Stevia rebaudiana Bertoni is a shrub having a high content of sweet diterpenoid glycosides in its leaves, mainly stevioside and rebaudioside A, which are used as noncaloric, natural sweeteners. The aim of this study was to deepen the knowledge about the insulin-mimetic effect exerted by four different mixtures of steviol glycosides, rich in stevioside and rebaudioside A, in neonatal rat cardiac fibroblasts. The potential antioxidant activity of these steviol glycosides was also assessed, as oxidative stress is associated with diabetes. Likewise the insulin effect, steviol glycosides caused an increase in glucose uptake into rat fibroblasts by activating the PI3K/Akt pathway, thus inducing Glut4 translocation to the plasma membrane. The presence of S961, an insulin antagonist, completely abolished these effects, allowing to hypothesize that steviol glycosides could act as ligands of the same receptor engaged by insulin. Moreover, steviol glycosides counteracted oxidative stress by increasing reduced glutathione intracellular levels and upregulating expression and activity of the two antioxidant enzymes superoxide dismutase and catalase. The present work unravels the insulin-mimetic effect and the antioxidant property exerted by steviol glycosides, suggesting their potential beneficial role in the cotreatment of diabetes and in health maintenance.

  17. Potential Roles of Stevia rebaudiana Bertoni in Abrogating Insulin Resistance and Diabetes: A Review.

    Science.gov (United States)

    Mohd-Radzman, Nabilatul Hani; Ismail, W I W; Adam, Zainah; Jaapar, Siti Safura; Adam, Aishah

    2013-01-01

    Insulin resistance is a key factor in metabolic disorders like hyperglycemia and hyperinsulinemia, which are promoted by obesity and may later lead to Type II diabetes mellitus. In recent years, researchers have identified links between insulin resistance and many noncommunicable illnesses other than diabetes. Hence, studying insulin resistance is of particular importance in unravelling the pathways employed by such diseases. In this review, mechanisms involving free fatty acids, adipocytokines such as TNF α and PPAR γ and serine kinases like JNK and IKK β , asserted to be responsible in the development of insulin resistance, will be discussed. Suggested mechanisms for actions in normal and disrupted states were also visualised in several manually constructed diagrams to capture an overall view of the insulin-signalling pathway and its related components. The underlying constituents of medicinal significance found in the Stevia rebaudiana Bertoni plant (among other plants that potentiate antihyperglycemic activities) were explored in further depth. Understanding these factors and their mechanisms may be essential for comprehending the progression of insulin resistance towards the development of diabetes mellitus.

  18. A Novel Diterpene Glycoside with Nine Glucose Units from Stevia rebaudiana Bertoni

    Directory of Open Access Journals (Sweden)

    Indra Prakash

    2017-01-01

    Full Text Available Following our interest in new diterpene glycosides with better taste profiles than that of Rebaudioside M, we have recently isolated and characterized Rebaudioside IX—a novel steviol glycoside—from a commercially‐supplied extract of Stevia rebaudiana Bertoni. This molecule contains a hexasaccharide group attached at C‐13 of the central diterpene core, and contains three additional glucose units when compared with Rebaudioside M. Here we report the complete structure elucidation—based on extensive Nuclear Magnetic Resonance (NMR analysis (1H, 13C, Correlation Spectroscopy (COSY, Heteronuclear Single Quantum Coherence‐Distortionless Enhancement Polarization Transfer (HSQC‐DEPT, Heteronuclear Multiple Bond Correlation (HMBC, 1D Total Correlation Spectroscopy (TOCSY, Nuclear Overhauser Effect Spectroscopy (NOESY and mass spectral data—of this novel diterpene glycoside with nine sugar moieties and containing a relatively rare 16 α‐linked glycoside. A steviol glycoside bearing nine glucose units is unprecedented in the literature, and could have an impact on the natural sweetener catalog.

  19. Potential Roles of Stevia rebaudiana Bertoni in Abrogating Insulin Resistance and Diabetes: A Review

    Science.gov (United States)

    Mohd-Radzman, Nabilatul Hani; Ismail, W. I. W.; Adam, Zainah; Jaapar, Siti Safura; Adam, Aishah

    2013-01-01

    Insulin resistance is a key factor in metabolic disorders like hyperglycemia and hyperinsulinemia, which are promoted by obesity and may later lead to Type II diabetes mellitus. In recent years, researchers have identified links between insulin resistance and many noncommunicable illnesses other than diabetes. Hence, studying insulin resistance is of particular importance in unravelling the pathways employed by such diseases. In this review, mechanisms involving free fatty acids, adipocytokines such as TNFα and PPARγ and serine kinases like JNK and IKKβ, asserted to be responsible in the development of insulin resistance, will be discussed. Suggested mechanisms for actions in normal and disrupted states were also visualised in several manually constructed diagrams to capture an overall view of the insulin-signalling pathway and its related components. The underlying constituents of medicinal significance found in the Stevia rebaudiana Bertoni plant (among other plants that potentiate antihyperglycemic activities) were explored in further depth. Understanding these factors and their mechanisms may be essential for comprehending the progression of insulin resistance towards the development of diabetes mellitus. PMID:24324517

  20. A Novel Diterpene Glycoside with Nine Glucose Units from Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Prakash, Indra; Ma, Gil; Bunders, Cynthia; Charan, Romila D; Ramirez, Catherine; Devkota, Krishna P; Snyder, Tara M

    2017-01-31

    Following our interest in new diterpene glycosides with better taste profiles than that of Rebaudioside M, we have recently isolated and characterized Rebaudioside IX-a novel steviol glycoside-from a commercially-supplied extract of Stevia rebaudiana Bertoni. This molecule contains a hexasaccharide group attached at C-13 of the central diterpene core, and contains three additional glucose units when compared with Rebaudioside M. Here we report the complete structure elucidation-based on extensive Nuclear Magnetic Resonance (NMR) analysis (1H, 13C, Correlation Spectroscopy (COSY), Heteronuclear Single Quantum Coherence-Distortionless Enhancement Polarization Transfer (HSQC-DEPT), Heteronuclear Multiple Bond Correlation (HMBC), 1D Total Correlation Spectroscopy (TOCSY), Nuclear Overhauser Effect Spectroscopy (NOESY)) and mass spectral data-of this novel diterpene glycoside with nine sugar moieties and containing a relatively rare 16 α-linked glycoside. A steviol glycoside bearing nine glucose units is unprecedented in the literature, and could have an impact on the natural sweetener catalog.

  1. Micropropagation and Biomass Production of True-to-Type Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Modi, Arpan R; Sharma, Vikas; Patil, Ghanshyam; Singh, Amritpal S; Subhash, N; Kumar, Nitish

    2016-01-01

    Here we describe an efficient micropropagation protocol for Stevia rebaudiana Bertoni. We present experiments carried out to optimize the suitable media for in vitro shoot multiplication and root induction and to study the effect of culture vessel on shoot multiplication. Among all different media tested for in vitro shoot multiplication, hormone-free liquid medium is most suitable. The highest number of nodes per shoot (5.4) and length of shoot (4.76 cm) at 4 weeks after subculturing are observed when single node explants are placed on modified MS medium supplemented with 1 % sucrose and 0.7 % agar. The highest response of multiplication rate (9.56) is observed on half strength of macroelement of MS with full strength of microelement of MS and 170 mg/l KH2PO4, and 185 mg/l MgSO4 in plastic growth container. Further, RAPD marker analysis of in vitro-raised plants maintained their clonal fidelity and true-to-type without showing any somaclonal variation.

  2. Glycosides from Stevia rebaudiana Bertoni Possess Insulin-Mimetic and Antioxidant Activities in Rat Cardiac Fibroblasts

    Science.gov (United States)

    Prata, Cecilia; Zambonin, Laura; Rizzo, Benedetta; Vieceli Dalla Sega, Francesco

    2017-01-01

    Stevia rebaudiana Bertoni is a shrub having a high content of sweet diterpenoid glycosides in its leaves, mainly stevioside and rebaudioside A, which are used as noncaloric, natural sweeteners. The aim of this study was to deepen the knowledge about the insulin-mimetic effect exerted by four different mixtures of steviol glycosides, rich in stevioside and rebaudioside A, in neonatal rat cardiac fibroblasts. The potential antioxidant activity of these steviol glycosides was also assessed, as oxidative stress is associated with diabetes. Likewise the insulin effect, steviol glycosides caused an increase in glucose uptake into rat fibroblasts by activating the PI3K/Akt pathway, thus inducing Glut4 translocation to the plasma membrane. The presence of S961, an insulin antagonist, completely abolished these effects, allowing to hypothesize that steviol glycosides could act as ligands of the same receptor engaged by insulin. Moreover, steviol glycosides counteracted oxidative stress by increasing reduced glutathione intracellular levels and upregulating expression and activity of the two antioxidant enzymes superoxide dismutase and catalase. The present work unravels the insulin-mimetic effect and the antioxidant property exerted by steviol glycosides, suggesting their potential beneficial role in the cotreatment of diabetes and in health maintenance. PMID:28947927

  3. Stevioside from Stevia rebaudiana Bertoni Increases Insulin Sensitivity in 3T3-L1 Adipocytes

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    Nabilatul Hani Mohd-Radzman

    2013-01-01

    Full Text Available Stevioside from Stevia rebaudiana has been reported to exert antihyperglycemic effects in both rat and human subjects. There have been few studies on these effects in vitro. In this paper, radioactive glucose uptake assay was implemented in order to assess improvements in insulin sensitivity in 3T3-L1 cells by elevation of glucose uptake following treatment with stevioside. Oil Red-O staining and MTT assay were utilized to confirm adipocyte differentiation and cell viability, respectively. Findings from this research showed a significant increase in absorbance values in mature adipocytes following Oil Red-O staining, confirming the differentiation process. Stevioside was noncytotoxic to 3T3-L1 cells as cell viability was reduced by a maximum of 17%, making it impossible to determine its IC50. Stevioside increased glucose uptake activities by 2.1 times (p<0.001 in normal conditions and up to 4.4 times (p<0.001 in insulin-resistant states. At times, this increase was higher than that seen in positive control group treated with rosiglitazone maleate, an antidiabetic agent. Expressions of pY20 and p-IRS1 which were measured via Western blot were improved by stevioside treatment. In conclusion, stevioside has direct effects on 3T3-L1 insulin sensitivity via increase in glucose uptake and enhanced expression of proteins involved in insulin-signalling pathway.

  4. Glycosides from Stevia rebaudiana Bertoni Possess Insulin-Mimetic and Antioxidant Activities in Rat Cardiac Fibroblasts

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    Cecilia Prata

    2017-01-01

    Full Text Available Stevia rebaudiana Bertoni is a shrub having a high content of sweet diterpenoid glycosides in its leaves, mainly stevioside and rebaudioside A, which are used as noncaloric, natural sweeteners. The aim of this study was to deepen the knowledge about the insulin-mimetic effect exerted by four different mixtures of steviol glycosides, rich in stevioside and rebaudioside A, in neonatal rat cardiac fibroblasts. The potential antioxidant activity of these steviol glycosides was also assessed, as oxidative stress is associated with diabetes. Likewise the insulin effect, steviol glycosides caused an increase in glucose uptake into rat fibroblasts by activating the PI3K/Akt pathway, thus inducing Glut4 translocation to the plasma membrane. The presence of S961, an insulin antagonist, completely abolished these effects, allowing to hypothesize that steviol glycosides could act as ligands of the same receptor engaged by insulin. Moreover, steviol glycosides counteracted oxidative stress by increasing reduced glutathione intracellular levels and upregulating expression and activity of the two antioxidant enzymes superoxide dismutase and catalase. The present work unravels the insulin-mimetic effect and the antioxidant property exerted by steviol glycosides, suggesting their potential beneficial role in the cotreatment of diabetes and in health maintenance.

  5. Chromosomal and morphological studies of diploid and polyploid cytotypes of Stevia rebaudiana (Bertoni Bertoni (Eupatorieae, Asteraceae

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    Vanessa M. de Oliveira

    2004-01-01

    Full Text Available In this study, we examined the chromosome number and some morphological features of strains of Stevia rebaudiana. The chromosomes were analyzed during mitosis and diakinesis, and the tetrad normality and pollen viability were also assessed. In addition, stomata and pollen were measured and some plant features were studied morphometrically. All of the strains had 2n = 22, except for two, which had 2n = 33 and 2n = 44. Pairing at diakinesis was n = 11II for all of the diploid strains, whereas the triploid and tetraploid strains had n = 11III and n = 11IV, respectively. Triploid and tetraploid plants had a lower tetrad normality rate than the diploids. All of the strains had inviable pollen. Thus, the higher the ploidy number, the greater the size of the pollen and the stomata, and the lower their number per unit area. The triploid strain produced the shortest plants and the lowest number of inflorescences, whereas the tetraploid strain had the largest leaves. Analysis of variance revealed highly significant differences among the strains, with a positive correlation between the level of ploidy and all of the morphological features examined.

  6. Potential Roles of Stevia rebaudiana Bertoni in Abrogating Insulin Resistance and Diabetes: A Review

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    Nabilatul Hani Mohd-Radzman

    2013-01-01

    Full Text Available Insulin resistance is a key factor in metabolic disorders like hyperglycemia and hyperinsulinemia, which are promoted by obesity and may later lead to Type II diabetes mellitus. In recent years, researchers have identified links between insulin resistance and many noncommunicable illnesses other than diabetes. Hence, studying insulin resistance is of particular importance in unravelling the pathways employed by such diseases. In this review, mechanisms involving free fatty acids, adipocytokines such as TNFα and PPARγ and serine kinases like JNK and IKKβ, asserted to be responsible in the development of insulin resistance, will be discussed. Suggested mechanisms for actions in normal and disrupted states were also visualised in several manually constructed diagrams to capture an overall view of the insulin-signalling pathway and its related components. The underlying constituents of medicinal significance found in the Stevia rebaudiana Bertoni plant (among other plants that potentiate antihyperglycemic activities were explored in further depth. Understanding these factors and their mechanisms may be essential for comprehending the progression of insulin resistance towards the development of diabetes mellitus.

  7. Light-induced biochemical variations in secondary metabolite production and antioxidant activity in callus cultures of Stevia rebaudiana (Bert).

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    Ahmad, Naveed; Rab, Abdur; Ahmad, Nisar

    2016-01-01

    Stevia rebaudiana (S. rebaudiana) is a very important species with worldwide medicinal and commercial uses. Light is one of the major elicitors that fluctuate morphogenic potential and biochemical responses. In the present study, we investigated the effect of various spectral lights on biomass accumulation and secondary metabolite production in callus cultures of S. rebaudiana. Leaf explants were placed on Murashige and Skoog (MS) medium and exposed to various spectral lights. 6-Benzyle adenine (BA) and 2, 4-dichlorophenoxy acetic acid (2, 4-D; 2.0 mgl(-1)) were used for callus induction. The control light (16/8h) produced optimum callogenic response (92.73%) than other colored lights. Compared to other colored lights, control grown cultures displayed maximum biomass accumulation (5.78 gl(-1)) during a prolonged log phase at the 18th day of growth kinetics. Cultures grown under blue light enhanced total phenolic content (TPC; 102.32 μg/g DW), total flavonoid content (TFC; 22.07 μg/g DW) and total antioxidant capacity (TAC; 11.63 μg/g DW). On the contrary, green and red lights improved reducing power assay (RPA; 0.71Fe(II)g(-1) DW) and DPPH-radical scavenging activity (DRSA; 80%). Herein, we concluded that the utilization of colored lights is a promising strategy for enhanced production of antioxidant secondary metabolites in callus cultures of S. rebaudiana. Copyright © 2015 Elsevier B.V. All rights reserved.

  8. The effects of aqueous extract of stevia plant (Stevia rebaudiana on serum concentration of vaspin and Angiopoietin-like Protein-3 in streptozotocin induced diabetic rats

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    Samad Akbarzadeh

    2015-05-01

    Full Text Available Background: Considering the importance of the prevalence of diabetes which involves approximately 6% of the world's adult population, the need for low-calorie natural sweetener is felt more than ever. Recent studies have shown that hormones such as vaspin and Angiopoietin-like Protein3 (ANGPTL3 are associated with diabetes. The purpose of this study is to evaluate the effect of Stevia (Stevia rebaudiana plant (as a low calorie sugar on serum concentration of vaspin and ANGPTL3 in diabetic rats. Materials and Methods: In this case-control study, forty male wistar rats weighing 180-250 g were divided into 5 equal groups: control, diabetic control and doses of 250, 500 and 750 mg/kg/BW/day of Stevia extract treatment. Diabetes was induced by intravenous injection of Streptozotocin (60 mg/kg. After 5 days, the rats with glucose above 300 mg/dl were considered as diabetic. The Stevia treatment groups received 250, 500 and 750 mg of Stevia extract for thirty days. At the end of experiment, blood samples were obtained measurement of vaspin, ANGPTL3, triglycerides, cholesterol, HDL, glucose, insulin and ALP. Histological study of the pancreas and liver biopsy were also performed. The results of the treatment and control groups were analyzed by SPSS software and P<0.05 was considered statistically significant. Results: The level of alkaline phosphatase, insulin resistance index, glucose and triglyceride were decreased significantly in the groups 250 and 500 compared with the diabetic control group(p≤0.05. However, insulin levels, HOMA.B, vaspin, ANGPTL3 and weight of the rats in all treatment groups were not significantly different from the control diabetic group. There were no histological changes in pancreatic and liver tissue following Stevia treatment. Conclusion: Administration of Stevia extract via reduction in serum glucose, triglyceride and insulin resistance can be effective in lowering the blood sugar and lipid, also lowering concentrations

  9. A functional (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase exhibits diurnal regulation of expression in Stevia rebaudiana (Bertoni).

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    Kumar, Hitesh; Kumar, Sanjay

    2013-09-15

    The leaves of stevia [Stevia rebaudiana (Bertoni)] are a rich source of steviol glycosides that are used as non-calorific sweetener in many countries around the world. Steviol moiety of steviol glycosides is synthesized via plastidial 2C-methyl-D-erythritol 4-phosphate pathway, where (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR) is the key enzyme. HDR catalyzes the simultaneous conversion of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate into five carbon isoprenoid units, isopentenyl diphosphate and dimethylallyl diphosphate. Stevia HDR (SrHDR) successfully rescued HDR lethal mutant strain MG1655 araispH upon genetic complementation, suggesting SrHDR to encode a functional protein. The gene exhibited diurnal variation in expression. To identify the possible regulatory elements, upstream region of the gene was cloned and putative cis-acting elements were detected by in silico analysis. Electrophoretic mobility shift assay, using a putative light responsive element GATA showed the binding of nuclear proteins (NP) isolated from leaves during light period of the day, but not with the NP from leaves during the dark period. Data suggested the involvement of GATA box in light mediated gene regulation of SrHDR in stevia. Copyright © 2013 Elsevier B.V. All rights reserved.

  10. Stevia rebaudiana Bertoni, A Rich Source of a Natural Sweetener: A Complete Review on the Biochemical, Nutritional and Functional Features

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    Leila Nikniaz

    2015-08-01

    Full Text Available Stevia rebaudiana Bertoni is an ancient perennial shrub of South American used since ancient times for various purposes throughout the world. Stevia extracts contain high levels of sweetening compounds called steviol glycosides which besides sweetening (300 times sweeter than sacarose possess antioxidant, antimicrobial and antifungal activity. Stevioside and rebaudioside A are the main sweetening compounds of this plant which are thermostable even at temperatures of up to 200 °C, making them suitable for use in cooked foods. Preparations of steviol glycosides are reported to be used principally as sweeteners in fruit and milk-based drinks, desserts, yoghurts, confectioneries, and pickles. The use of Stevia extract as a table-top sweetener is well known. The leaves of Stevia has functional and sensory properties superior to those of many other high-potency sweeteners and is likely to become a major source of safe and high-potency sweetener for the growing natural food market in the future.​

  11. The Effects of Various LED Light Wavelengths to the Physiological and Morphological Parameters of Stevia (Stevia rebaudiana Bertoni

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    Esra UCAR

    2016-09-01

    Full Text Available In this study, it was investigated the growth of stevia (Stevia rebaudiana Bertoni under various wavelengths of LED lamp (Light Emitting Diodes, which can emit daylight (cool white; 400–700 nm, red (620–630 nm and blue (465–485 nm wavelengths of the light in the electromagnetic spectrum. In all applications, quantity of PAR (photosynthetically active radiation was adjusted as 150 µmol.m-2 s-1. Study had maintained in plant breeding cabin 16 hours light and 8 hours dark environment. Results demonstrated that while the highest plant height was determined in the “30% blue light+ 70% red light” application, the highest stem length was found in the “50% blue light + 50% red light” application. In addition, the number of the stems reached the highest value in the “70% blue light + 30% red light” application. Consequently, a correlation was observed between negative “a” value and the amount of chlorophyll. Because of the hereby obtained results, comparing to other applications, the “50% blue light + 50% red light” was found as the best light application to obtain optimum yield values of stevia.

  12. Comparação de diferentes metodologias para obtenção de cultura de calos de Stevia rebaudiana (Bertoni Bertoni = Comparison of different methodologies for obtaining callus cultures from Stevia rebaudiana (Bertoni Bertoni

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    Ana Paula Patrão

    2007-07-01

    Full Text Available As folhas de Stevia rebaudiana (Bertoni Bertoni (Asteraceae contêm glicosídeos diterpenóides (GDS, que são cerca de 300 vezes mais doce que a sacarose a 4%. O objetivo deste estudo foi avaliar a formação de calos, a partir de folhas obtidas in vivo e in vitro de S. rebaudiana em dois meios já descritos na literatura: Murashige e Skoog (MS, suplementado com 3 mg L-1 de ácido 2,4-diclorofenóxiacético (2,4-D, e o MS suplementado com 1 mg L-1 de ácido naftalenoacético (ANA e 0,5 mg L-1 de 6-benzilaminopurina 6-BAP e um desenvolvido em nosso laboratório o Woody Plant Medium (WPM, suplementado com 6 mg L-1 de ANA e 4 mg L-1 de cinetina (CIN. Os explantes obtidos in vitro iniciaram a formação de calos um pouco mais rapidamente que os das folhas de plantas advindas da natureza. A utilização dos nutrientes do meio WPM, associada a uma combinação de fitorreguladores adequada, proporcionou velocidade de indução e multiplicação de calos bem maiores que as apresentadas nos meios que empregaram os nutrientes do MS. Novos experimentos serão realizados, depois de alcançada a estabilidade genética dos calos, visando avaliar a capacidade destes em biossintetizar os GDS.The leaves from Stevia rebaudiana (Bertoni Bertoni (Asteraceae contain diterpenoid glycosides (GDS, which are almost 300 times sweeter than sucrose at 4%. The subject of this study was to evaluate the callus-formation from in vivo and in vitro leaves of Stevia rebaudiana in two already described in literature: Murashige and Skoog (MS supplemented with 3 mg L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D; MS supplemented with 1 mg L-1 of naphthaleneacetic acid (NAA and 0.5 mg L-1 of 6-benzylaminopurine (6-BAP and other developed in our laboratory the Woody Plant Medium (WPM with 6 mg L-1 of NAA and 4 mg L-1 of Kinetin (KIN. The explants obtained in vitro initiated callus formation faster than leaves from natural plants. The utilization of WPM nutrients, associated with an

  13. Antidiabetic activity of medium-polar extract from the leaves of Stevia rebaudiana Bert. (Bertoni) on alloxan-induced diabetic rats

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    Misra, Himanshu; Soni, Manish; Silawat, Narendra; Mehta, Darshana; Mehta, B. K.; Jain, D. C.

    2011-01-01

    Objective: To investigate the medicative effects of medium-polar (benzene:acetone, 1:1, v/v) extract of leaves from Stevia rebaudiana (family Asteraceae) on alloxan-induced diabetic rats. Materials and Methods: Diabetes was induced in adult albino Wistar rats by intraperitoneal (i.p.) injection of alloxan (180 mg/kg). Medium-polar extract was administered orally at daily dose of 200 and 400 mg/kg body wt. basis for 10 days. The control group received normal saline (0.9%) for the same duration. Glibenclamide was used as positive control reference drug against Stevia extract. Results: Medium-polar leaf extract of S. rebaudiana (200 and 400 mg/kg) produced a delayed but significant (P Stevia extract was found to antagonize the necrotic action of alloxan and thus had a re-vitalizing effect on β-cells of pancreas. PMID:21687353

  14. Stevia rebaudiana (Bert Bertoni: influence of osmotic stress and seed priming on seed germination under laboratory conditions

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    Keila Regina Hossa

    2017-05-01

    Full Text Available The foremost factor necessary for plant growers cultivating large acreages of Stevia rebaudiana (Bert Bertoni is the production of qualitative bedding plants. The objective of this study was to evaluate the influence of osmotic-priming on the uniformity of seed germination. First, we evaluated the percentage of normal seedlings from two seed samples harvested in 2011 and 2012. The seeds harvested in 2012 produced 71.4% normal seedlings and thus they were used in the next experiments. The seeds were subjected to osmotic stress using five concentrations of polyethylene glycol (PEG-6000 at -0.2, -0.4, -0.6, -0.8, and -1.0 MPa in contrast with distilled water. Based on these first results, only -0.8 and -1.0 MPa were evaluated in the third experiment. The seeds were immersed in both concentrations of polyethylene glycol (PEG-6000 for imbibing at 20ºC for four, five, six, and seven days. Thereafter, we evaluated the time to the first normal seedling (Ti, time to the last normal seedling (Tf, percentage normal seedlings at the initial time (Pi and percentage of normal seedlings at the end of every treatment (Pf. Osmotic priming increased the percentage of normal seedlings of the Stevia rebaudiana and reduced the time to the first and last germination events.

  15. EFFECT OF GENOTYPE ON THE in vitro REGENERATION OF Stevia rebaudiana VIA SOMATIC EMBRYOGENESIS

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    Esther Julia Naranjo

    2015-11-01

    Full Text Available ABSTRACTStevia rebaudiana (Asteraceae is a plant of economic importance because of its medicinal properties and the presence of sweetener compounds on its leaves. These compounds can be a substitute for sucrose in a wide variety of products used by persons with diabetes and obesity problems. To standardize an efficient and effective propagation method for the different Stevia genotypes grown in Colombia, this study evaluated the effect of different combinations of the plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D, indole-3-acetic acid (IAA, indole-3-butyric acid (IBA, 6-(gamma, gamma-dimethylallylamino purine (2iP and Zeatin on the induction and development of somatic embryos. Adenine and coconut water were also evaluated as supplements in the basal culture medium Murashige and Skoog Basal Salt Mixture (MS with glutamine. The combination of 2,4-D (18.09 μM and 2iP (7.38 μM produced the highest number of somatic embryos per explant, which had well-defined characteristics. The genotype showed a significant effect on the embryogenic response. In the “SRQ-93” genotype, the formation and development of somatic embryos was achieved, whereas the genotypes “Bertoni” and “Morita II” only yielded embryogenic and non-embryogenic calli, respectively. The conversion to seedlings was achieved on the regeneration medium containing gibberellic acid (GA3 (0.29 μM and activated charcoal. RESUMENStevia rebaudiana (Asteraceae, es una planta de gran importancia económica debido a sus propiedades medicinales y a la presencia de compuestos endulzantes en sus hojas, los cuales pueden sustituir la sacarosa en gran variedad de productos utilizados por personas con problemas de diabetes y obesidad. Con el propósito de estandarizar un método de propagación eficiente y efectivo para diferentes genotipos de Stevia cultivados en Colombia, en la presente investigación se evaluó el efecto sobre la inducción y desarrollo de embriones som

  16. [Research on the Spectrophotometry for Determination of Flavonoids in Waste Residue of Stevia Rebaudiana].

    Science.gov (United States)

    Luo, Xu-yan; Fang, Hao-bin; Huang, Rui-yu; Zhao, Dong-fang; Zhu, Ting; Guo, Yang-yang; Zeng Yi-ping; Liu, Ren-yue; Zhu, Dong

    2015-10-01

    A spectrophotometric method for the determination of flavonoids content in Waste Residue of Stevia Rebaudiana was established by comparing the UV-Vis spectra with three spectrophotometric methods: Direct, AlCl3 and NaNO2-Al (NO3)3-NaOH method. The effects of spectrophotometric reaction were studied by analyzing the wavelength, the dosage of color development reagent, reaction time, temperature and pH. The further research was studied by analyzing linearity between the absorbance and the concentration, the stability of this color system, the recovery rate and so on. The results showed that NaNO2-Al(NO3)3-NaOH method was best. The wavelength was 500 nm. The standard sample was rutin. There was a good linear relationship in the ranges of 2 - 24 μg x mL(-1), correlation coefficient R2 = 0.999 5. The recovery of the additional sample was 96.0%-104.3%. The relative standard deviation(RSD) is respectively 3.75%. The dosages of NaNO2 and Al(NO3)3 both were 1.0 mL, both of their reaction time was 6 min. The dosage of NaOH was 15.0 mL and the reaction time was 15 min. The temperature in the whole process of the reaction must be maintained at 40 degrees C and pH is 5. The spectrophotometric condition was further improved on the determination of flavonoids content. This method, which is simple, rapid and accuracy, can be used in practical application.

  17. Agrobacterium Mediated Transient Gene Silencing (AMTS) in Stevia rebaudiana: Insights into Steviol Glycoside Biosynthesis Pathway

    Science.gov (United States)

    Guleria, Praveen; Yadav, Sudesh Kumar

    2013-01-01

    Background Steviol glycoside biosynthesis pathway has emerged as bifurcation from ent-kaurenoic acid, substrate of methyl erythritol phosphate pathway that also leads to gibberellin biosynthesis. However, the genetic regulation of steviol glycoside biosynthesis has not been studied. So, in present study RNA interference (RNAi) based Agrobacterium mediated transient gene silencing (AMTS) approach was followed. SrKA13H and three SrUGTs (SrUGT85C2, SrUGT74G1 and SrUGT76G1) genes encoding ent-kaurenoic acid-13 hydroxylase and three UDP glycosyltransferases of steviol glycoside biosynthesis pathway were silenced in Stevia rebaudiana to understand its molecular mechanism and association with gibberellins. Methodology/Principal Findings RNAi mediated AMTS of SrKA13H and three SrUGTs has significantly reduced the expression of targeted endogenous genes as well as total steviol glycoside accumulation. While gibberellins (GA3) content was significantly enhanced on AMTS of SrUGT85C2 and SrKA13H. Silencing of SrKA13H and SrUGT85C2 was found to block the metabolite flux of steviol glycoside pathway and shifted it towards GA3 biosynthesis. Further, molecular docking of three SrUGT proteins has documented highest affinity of SrUGT76G1 for the substrates of alternate pathways synthesizing steviol glycosides. This could be a plausible reason for maximum reduction in steviol glycoside content on silencing of SrUGT76G1 than other genes. Conclusions SrKA13H and SrUGT85C2 were identified as regulatory genes influencing carbon flux between steviol glycoside and gibberellin biosynthesis. This study has also documented the existence of alternate steviol glycoside biosynthesis route. PMID:24023961

  18. Selection of suitable propagation method for consistent plantlets production in Stevia rebaudiana (Bertoni)

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    Khalil, Shahid Akbar; Zamir, Roshan; Ahmad, Nisar

    2014-01-01

    Stevia rebaudiana (Bert.) is an emerging sugar alternative and anti-diabetic plant in Pakistan. That is why people did not know the exact time of propagation. The main objective of the present study was to establish feasible propagation methods for healthy biomass production. In the present study, seed germination, stem cuttings and micropropagation were investigated for higher productivity. Fresh seeds showed better germination (25.51–40%) but lost viability after a few days of storage. In order to improve the germination percentage, seeds were irradiated with 2.5, 5.0, 7.5 and 10 Gy gamma doses. But gamma irradiation did not show any significant change in seed germination. A great variation in survival of stem cutting was observed in each month of 2012. October and November were found the most suitable months for stem cutting survival (60%). In order to enhance survival, stem cuttings were also dipped in different plant growth regulators (PGRs) solution. Only indole butyric acid (IBA; 1000 ppm) treated cutting showed a higher survival (33%) than control (11.1%). Furthermore, simple and feasible indirect regeneration system was established from leaf explants. Best callus induction (84.6%) was observed on MS-medium augmented with 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D; 2.0 mg l−1). For the first time, we obtained the highest number of shoots (106) on a medium containing BA (1.5 mg l−1) and gibberellic acid (GA3; 0.5 mg l−1). Plantlets were successfully acclimatized in plastic pots. The current results preferred micropropagation (85%) over seed germination (25.51–40%) and stem cutting (60%). PMID:25473365

  19. Agrobacterium mediated transient gene silencing (AMTS in Stevia rebaudiana: insights into steviol glycoside biosynthesis pathway.

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    Praveen Guleria

    Full Text Available BACKGROUND: Steviol glycoside biosynthesis pathway has emerged as bifurcation from ent-kaurenoic acid, substrate of methyl erythritol phosphate pathway that also leads to gibberellin biosynthesis. However, the genetic regulation of steviol glycoside biosynthesis has not been studied. So, in present study RNA interference (RNAi based Agrobacterium mediated transient gene silencing (AMTS approach was followed. SrKA13H and three SrUGTs (SrUGT85C2, SrUGT74G1 and SrUGT76G1 genes encoding ent-kaurenoic acid-13 hydroxylase and three UDP glycosyltransferases of steviol glycoside biosynthesis pathway were silenced in Stevia rebaudiana to understand its molecular mechanism and association with gibberellins. METHODOLOGY/PRINCIPAL FINDINGS: RNAi mediated AMTS of SrKA13H and three SrUGTs has significantly reduced the expression of targeted endogenous genes as well as total steviol glycoside accumulation. While gibberellins (GA3 content was significantly enhanced on AMTS of SrUGT85C2 and SrKA13H. Silencing of SrKA13H and SrUGT85C2 was found to block the metabolite flux of steviol glycoside pathway and shifted it towards GA3 biosynthesis. Further, molecular docking of three SrUGT proteins has documented highest affinity of SrUGT76G1 for the substrates of alternate pathways synthesizing steviol glycosides. This could be a plausible reason for maximum reduction in steviol glycoside content on silencing of SrUGT76G1 than other genes. CONCLUSIONS: SrKA13H and SrUGT85C2 were identified as regulatory genes influencing carbon flux between steviol glycoside and gibberellin biosynthesis. This study has also documented the existence of alternate steviol glycoside biosynthesis route.

  20. PRODUKSI LOW CALORIE SWEET BIO-YOGHURT DENGAN PENAMBAHAN EKSTRAK DAUN STEVIA (Stevia rebaudiana SEBAGAI PENGGANTI GULA (Production of Low Calorie Sweet Bio-Yoghurt with The Addition of Stevia’s Leaf Extract (Stevia rebaudiana for Sugar Substitution

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    Widodo Widodo

    2015-11-01

    penampilan dan warna antara yoghurt dengan penambahan sweetener gula dibandingkan dengan ekstrak daun stevia, tetapi penambahan ekstrak daun stevia berpengaruh terhadap aroma, rasa, mouth-feel, dan daya terima. Penambahan ekstrak daun stevia dapat mempertahankan viabilitas bakteri asam laktat dan probiotik dalam yoghurt selama seminggu. Low calorie sweet bio-yoghurt dengan penambahan 0,5% ekstrak daun stevia menghasilkan daya terima terbaik bagi panelis. Kata kunci: Low calorie sweet bio-yoghurt, daun stevia, pengganti gula

  1. Isolation and characterization of inulin with a high degree of polymerization from roots of Stevia rebaudiana (Bert.) Bertoni.

    Science.gov (United States)

    Lopes, Sheila M S; Krausová, Gabriela; Rada, Vojtěch; Gonçalves, José E; Gonçalves, Regina A C; de Oliveira, Arildo J B

    2015-06-26

    The polysaccharide inulin has great importance in the food and pharmaceutical industries. The degree of polymerization (DP) of inulin influences important properties, such as, solubility, thermal stability, sweetness power and prebiotic activity. Molecules with a high degree of polymerization are obtained through physical techniques for enrichment of the inulin chains because they are not commonly obtained from plants extract. Gas chromatography/Mass Spectrometry and (1)H Nuclear Magnetic Resonance analysis showed that inulin from Stevia rebaudiana roots has a degree of polymerization (DPn 28) higher than the value of DPn 12-15 for inulins from other plant species. Furthermore, the methodology of freeze/thaw to enrich the chains allowed us to increase the DP, similarly to other methodologies used for the enrichment of inulin chains. The prebiotic assays confirm that inulin from S. rebaudiana has a high DP. The combined use of these molecules with low degree of polymerization fructans seems to be advantageous to prolong the prebiotic effect in the colon. Our results suggest that S. rebaudiana roots are a promising source of high degree polymerization inulins. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Gene transcription and steviol glycoside accumulation in Stevia rebaudiana under polyethylene glycol-induced drought stress in greenhouse cultivation.

    Science.gov (United States)

    Hajihashemi, Shokoofeh; Geuns, Jan M C

    2016-09-01

    Stevia rebaudiana is a sweet herb of the Astraceae family, which is cultivated for the natural sweeteners it contains. The aim of this study was to assess the effect of drought, simulated by the application of polyethylene glycol (5%, 10%, and 15% w/v), on the content of steviol glycosides (SVglys) and transcription levels of six genes involved in the biosynthesis of these natural sweeteners. The transcription levels of ent-kaurene synthase, ent-kaurene oxidase, ent-kaurenoic acid hydroxylase, and three UDP-dependent glycosyltransferases, UGT85C2,UGT74G1 and UGT76G1 were downregulated under polyethylene glycol treatment. Polyethylene glycol treatment significantly decreased the amount of stevioside, rebaudioside A, B, C and F, steviolbioside, dulcoside A, rubusoside, and total SVglys. These results strongly suggest a close relationship of SVglys content with the transcription of genes involved in the SVglys biosynthesis pathway. Comparing the observations of the present study with other reports provided the knowledge that the Stevia response to drought stress can be influenced by different environmental and experimental factors, in addition to intensity of drought stress. In conclusion, these results strongly suggest that polyethylene glycol-induced drought stress has a negative effect on the content of SVglys and transcription of SVglys biosynthetic genes and that this should be investigated further. We recommend that sufficient irrigation of Stevia is required to obtain a high content of SVglys.

  3. 2C-Methyl- D- erythritol 2,4-cyclodiphosphate synthase from Stevia rebaudiana Bertoni is a functional gene.

    Science.gov (United States)

    Kumar, Hitesh; Singh, Kashmir; Kumar, Sanjay

    2012-12-01

    Stevia [Stevia rebaudiana (Bertoni)] is a perennial herb which accumulates sweet diterpenoid steviol glycosides (SGs) in its leaf tissue. SGs are synthesized by 2C-methyl-D-erythritol 4-phosphate (MEP) pathway. Of the various enzymes of the MEP pathway, 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MDS) (encoded by MDS) catalyzes the cyclization of 4-(cytidine 5' diphospho)-2C-methyl-D-erythritol 2-phosphate into 2C-methyl-D-erythritol 2,4-cyclodiphosphate. Complementation of the MDS knockout mutant strain of Escherichia coli, EB370 with putative MDS of stevia (SrMDS) rescued the lethal mutant, suggesting SrMDS to be a functional gene. Experiments conducted in plant growth chamber and in the field suggested SrMDS to be a light regulated gene. Indole 3-acetic acid (IAA; 50, 100 μM) down-regulated the expression of SrMDS at 4 h of the treatment, whereas, abscisic acid did not modulate its expression. A high expression of SrMDS was observed during the light hours of the day as compared to the dark hours. The present work established functionality of SrMDS and showed the role of light and IAA in regulating expression of SrMDS.

  4. Stevia rebaudiana Bertoni as a natural antioxidant/antimicrobial for high pressure processed fruit extract: processing parameter optimization.

    Science.gov (United States)

    Barba, Francisco José; Criado, María Nieves; Belda-Galbis, Clara Miracle; Esteve, María José; Rodrigo, Dolores

    2014-04-01

    Response surface methodology was used to evaluate the optimal high pressure processing treatment (300-500 MPa, 5-15 min) combined with Stevia rebaudiana (Stevia) addition (0-2.5% (w/v)) to guarantee food safety while maintaining maximum retention of nutritional properties. A fruit extract matrix was selected and Listeria monocytogenes inactivation was followed from the food safety point of view while polyphenoloxidase (PPO) and peroxidase (POD) activities, total phenolic content (TPC) and antioxidant capacity (TEAC and ORAC) were studied from the food quality point of view. A combination of treatments achieved higher levels of inactivation of L. monocytogenes and of the oxidative enzymes, succeeding in completely inactivating POD and also increasing the levels of TPC, TEAC and ORAC. A treatment of 453 MPa for 5 min with a 2.5% (w/v) of Stevia succeeded in inactivating over 5 log cycles of L. monocytogenes and maximizing inactivation of PPO and POD, with the greatest retention of bioactive components. Copyright © 2013 Elsevier Ltd. All rights reserved.

  5. Synthesis of rebaudioside-A by enzymatic transglycosylation of stevioside present in the leaves of Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Adari, Bhaskar Rao; Alavala, Sateesh; George, Sara A; Meshram, Harshadas M; Tiwari, Ashok K; Sarma, Akella V S

    2016-06-01

    Rebaudioside-A is the second most abundant sweet diterpene glycoside (1-3%) present in the leaves of Stevia rebaudiana Bertoni, and is now being considered as a possible sucrose substitute due to its pleasant organoleptic properties and associated health benefits. In the present study, a novel in situ enzymatic transglycosylation of stevioside has been developed by pre-treating the stevia leaves with cellulase and adding soluble starch as the glucosyl donor. The results confirm that the transglycosylation of stevioside led to an enrichment in the rebaudioside-A content from 4% to 66%. This was further purified by multiple column chromatography to obtain 95% pure rebaudioside-A. The isolated rebaudioside-A showed concentration-dependent α-glucosidase inhibitory activity with IC50=35.01 μg/ml. Thus the study highlights the biotransformation of stevioside present in stevia leaves to rebaudioside-A by a simple, inexpensive and eco-friendly process that has commercial potential. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Efficient regeneration for enhanced steviol glycosides production in Stevia rebaudiana (Bertoni).

    Science.gov (United States)

    Aman, Nazish; Hadi, Fazal; Khalil, Shahid Akbar; Zamir, Roshan; Ahmad, Nisar

    2013-10-01

    An efficient method of regeneration for antidiabetic plant (Stevia rebaudiana) has been established for healthy biomass and main steviol glycosides (SGs) production, using different PGRs and agar concentrations. Higher callus induction (93.3%) was recorded when leaf explants were placed on an MS medium supplemented with 3.5 gL(-1) agar and 2.0 mgL(-1) 2,4-D. The addition of 7.0 gL(-1) agar and BA (1.0, 2.0 and 4.0 mgL(-1)) significantly (P<0.01) influences shooting response (100%). A maximum mean shoot length (13.03 cm) and 28 shoots per explant were observed on a medium containing 1.0 mgL(-1) BA. However, the maximum number of leaves (132.67) was encouraged by the addition of BA (1.0 mgL(-1)) and Kin (1.0 mgL(-1)). Lower agar (3.5 gL(-1)), IAA (2.0 mgL(-1)), and NAA (2.0 mgL(-1)) concentrations significantly influence the rooting percent (100%), the mean root length (2.9 cm), and the number of roots per plantlet (26.3). These plantlets were successfully acclimatized in the soil. The BA (3.0 mgL(-1)) in combination with Kin (3.0 mgL(-1)) and 3.5 gL(-1) agar increases dulcoside-A content (Dul-A; 71.8 μg/g-DW) in shoots compared to control (50.81 μg/g-DW). Similar PGRs with 7.0 gL(-1) significantly increases the production of steviosides (Stev. 82.48 μg/g-DW). A higher rebaudioside-A content (Reb-A; 12.35 μg/g-DW) was observed in shoots that underwent the addition of BA (1.0 mgL(-1)) and 7.0 gL(-1) agar than in control (07.39 μg/g-DW). Hereby, we developed an efficient and cost-effective method for regeneration and major SGs production, which could be helpful for future studies on this species. Copyright © 2013. Published by Elsevier SAS.

  7. Study of Stevia (Stevia rebaudiana Bertoni as a natural sweetener and its use in benefit of the health

    Directory of Open Access Journals (Sweden)

    Rebeca Salvador-Reyes

    2014-09-01

    Full Text Available Stevia, also known as "The miracle sweetener", is considered the best sugar substitute because it is up to 300 times sweeter and contains no calories. This shrub, grown in the forests of Brazil and Paraguay, has in its composition a high percentage of steviol glycosides (stevioside and rebaudioside A, which give it an intensely sweet taste and therapeutic properties against diabetes, hypertension and obesity also helps control weight, satiety and hunger. For its content in phenolic compounds, Stevia also acts as an excellent antioxidant and anticarcinogenic; also has been shown to have antibacterial, contraceptive, and diuretic properties. This review paper aims to collect information on the studies undertaken to stevia as an alternative to sugar and the benefits it provides consumption.

  8. Antihyperglycemic and Antihyperlipidemic Activity of Hydroponic Stevia rebaudiana Aqueous Extract in Hyperglycemia Induced by Immobilization Stress in Rabbits.

    Science.gov (United States)

    Aghajanyan, Anush; Movsisyan, Zaruhi; Trchounian, Armen

    2017-01-01

    Diabetes mellitus (DM) is a serious worldwide problem related to human hyperglycemia. Thus, herbal preparations with antihyperglycemic properties especially leaf extracts of hydroponic Stevia rebaudiana (SR) would be useful in hyperglycemia treatment. The antihyperglycemic potential of this medicinal plant grown using hydroponics methods has been evaluated. Significant reduction of some biochemical characteristics for sugars and fatty acids in blood, liver, and muscle especially fasting glucose levels, serum triglycerides, LDL-cholesterol, total cholesterol levels, and increased HDL-cholesterol ones was shown with SR aqueous extract treatment. Therefore, the aqueous extract of SR is suggested to have antihyperglycemic and antihyperlipidemic activity and to restore liver and muscle glycogen levels (hepatoprotective effects) in hyperglycemia induced by immobilization stress in rabbits and might be recommended for treatment of DM (hyperglycemia).

  9. Antihyperglycemic and Antihyperlipidemic Activity of Hydroponic Stevia rebaudiana Aqueous Extract in Hyperglycemia Induced by Immobilization Stress in Rabbits

    Science.gov (United States)

    Aghajanyan, Anush; Movsisyan, Zaruhi

    2017-01-01

    Diabetes mellitus (DM) is a serious worldwide problem related to human hyperglycemia. Thus, herbal preparations with antihyperglycemic properties especially leaf extracts of hydroponic Stevia rebaudiana (SR) would be useful in hyperglycemia treatment. The antihyperglycemic potential of this medicinal plant grown using hydroponics methods has been evaluated. Significant reduction of some biochemical characteristics for sugars and fatty acids in blood, liver, and muscle especially fasting glucose levels, serum triglycerides, LDL-cholesterol, total cholesterol levels, and increased HDL-cholesterol ones was shown with SR aqueous extract treatment. Therefore, the aqueous extract of SR is suggested to have antihyperglycemic and antihyperlipidemic activity and to restore liver and muscle glycogen levels (hepatoprotective effects) in hyperglycemia induced by immobilization stress in rabbits and might be recommended for treatment of DM (hyperglycemia). PMID:28758125

  10. Current and New Insights in the Sustainable and Green Recovery of Nutritionally Valuable Compounds from Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Koubaa, Mohamed; Roselló-Soto, Elena; Šic Žlabur, Jana; Režek Jambrak, Anet; Brnčić, Mladen; Grimi, Nabil; Boussetta, Nadia; Barba, Francisco J

    2015-08-12

    The South American plant Stevia rebaudiana Bertoni is a great source of noncaloric sweeteners (steviol glycosides), mainly concentrated in its leaves, but also has important antioxidant compounds (vitamin C, polyphenols, chlorophylls, and carotenoids) and other important macro- and micronutrients such as folic acid and all of the essential amino acids except tryptophan. Traditionally, conventional methods have been used to recover nutritionally valuable compounds from plant food matrices. However, nowadays, the need for obtaining greener, sustainable, and viable processes has led both food industries and food scientists to develop new processes in full correspondence with the green extraction concept. This review focuses on some of the most promising nonconventional and emerging technologies, which may constitute a potential alternative to conventional methods or even could be combined to obtain a synergistic effect, thus reducing extraction time as well as solvent consumption and avoiding the use of toxic solvents.

  11. Antihyperglycemic and Antihyperlipidemic Activity of Hydroponic Stevia rebaudiana Aqueous Extract in Hyperglycemia Induced by Immobilization Stress in Rabbits

    Directory of Open Access Journals (Sweden)

    Anush Aghajanyan

    2017-01-01

    Full Text Available Diabetes mellitus (DM is a serious worldwide problem related to human hyperglycemia. Thus, herbal preparations with antihyperglycemic properties especially leaf extracts of hydroponic Stevia rebaudiana (SR would be useful in hyperglycemia treatment. The antihyperglycemic potential of this medicinal plant grown using hydroponics methods has been evaluated. Significant reduction of some biochemical characteristics for sugars and fatty acids in blood, liver, and muscle especially fasting glucose levels, serum triglycerides, LDL-cholesterol, total cholesterol levels, and increased HDL-cholesterol ones was shown with SR aqueous extract treatment. Therefore, the aqueous extract of SR is suggested to have antihyperglycemic and antihyperlipidemic activity and to restore liver and muscle glycogen levels (hepatoprotective effects in hyperglycemia induced by immobilization stress in rabbits and might be recommended for treatment of DM (hyperglycemia.

  12. Effect of Stevia (Stevia rebaudiana as a Substitute for Sugar on Physicochemical, Rheological and Sensory Properties of Dietary Saffron Syrup

    Directory of Open Access Journals (Sweden)

    Neda Hashemi

    2015-01-01

    Full Text Available In recent years production and usage of functional food and drinks is one of the public and scientist’s interest. Since carbohydrates have been recognizedas the major calorie source in many food baskets, most attention has been focused on substitution of sucrose with non-caloric sweeteners. Although both natural and synthetic sweeteners have been used in the formulation of food stuffs, deep concern is raised regarding safety aspects of synthetic ones. Stevia is a natural suitable sweetener that is currently being used as a substitute for sugar in many dietary and functional foods and drinks. In the present study the effect of using stevia sugar as a replacement for sucrose in the preparation of saffron beverages was investigated. Accordingly, saffron syrup was prepared with 5 treatments in which100%, 75%, 50%, 25% and 0% of stevia sugar replaced sucrose. Then, the physicochemical, rheological and sensory properties of saffron functional syrup were investigated. The results indicated that by increasing stevia brix level, density and viscosity increased but the syrups’ pH decreases. The best treatment was the sample containing 25% steviaand 75% sucrose that compared to other samples regarding sensory characteristics had a higher acceptance by consumer and regarding physicochemical and rheological characteristics more similar to the control sample.

  13. Impact of blue, red, and far-red light treatments on gene expression and steviol glycoside accumulation in Stevia rebaudiana.

    Science.gov (United States)

    Yoneda, Yuki; Nakashima, Hiroshi; Miyasaka, Juro; Ohdoi, Katsuaki; Shimizu, Hiroshi

    2017-05-01

    Stevia rebaudiana (Bertoni) Bertoni is a plant that biosynthesizes a group of natural sweeteners that are up to approximately 400 times sweeter than sucrose. The sweetening components of S. rebaudiana are steviol glycosides (SGs) that partially share their biosynthesis pathway with gibberellins (GAs). However, the molecular mechanisms through which SGs levels can be improved have not been studied. Therefore, transcription levels of several SG biosynthesis-related genes were analyzed under several light treatments involved in GA biosynthesis. We detected higher transcription of UGT85C2, which is one of the UDP-glycosyltransferases (UGTs) involved in catalyzing the sugar-transfer reaction, under red/far-red (R/FR) 1.22 light-emitting diodes (LEDs) and blue LEDs treatment. In this study, it was demonstrated that transcription levels of SG-related genes and the SGs content are affected by light treatments known to affect the GA contents. It is expected that this approach could serve as a practical way to increase SG contents using specific light treatments. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Effect of Nitrogen Fertilization and Harvest Time on Steviol Glycosides, Flavonoid Composition, and Antioxidant Properties in Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Tavarini, Silvia; Sgherri, Cristina; Ranieri, Anna Maria; Angelini, Luciana G

    2015-08-12

    This work investigated the effect of nitrogen fertilization and harvest time on the flavonoid composition and antioxidant properties of Stevia rebaudiana leaves. At the same time, changes in stevioside (Stev) and rebaudioside A (RebA) contents were recorded. A pot trial under open air conditions was set up, testing five N rates and three harvest times. The results showed that, by using an adequate N rate and choosing an appropriate harvest time, it was possible to significantly increase and optimize the bioactive compound levels. In particular, higher RebA, RebA/Stev ratio, total phenols and flavonoids, luteolin-7-O-glucoside, and apigenin-7-O-glucoside levels and antioxidant capacity were recorded by supplying 150 kg N ha(-1). Reduced or increased N availability in comparison with N150 had no consistent effect on Stevia phytochemicals content. Significant correlations were also found between stevioside and some of the flavonoids, indicating a possible role of flavonoids in the stevioside metabolic pathway, which deserves more investigations.

  15. Pesquisa de adocantes não calóricos sintéticos em adoçante natural de Stevia rebaudiana (Bert. Bertoni

    Directory of Open Access Journals (Sweden)

    Mariangela T. Auricchio

    Full Text Available Foram analisadas 19 amostras de "Adoçantes Natu rais de Stevia", tendo por objetivo verificar-se a presença de outros adoçantes nao calóricos sintéticos que não são declarados no rótulo do produto. Constatou-se que em 18 amostras,além de componentes do extrato de Stevia reubadiana (Bert. Bertoni, a sacarina estava presente e que só uma delas declarava tal fato no rótulo. Em três dessas amostras foi encontrado tanbém aspartame e em uma, ciclamato. Fimalmente, em uma amostra não foi encontrado nenhum componente do extrato de Stevia rebaudiana (Bert. Bertoni, mas sim uma mistuna de manitol e aspartame.

  16. Fortification of the whey protein isolate antioxidant and antidiabetic activity with fraction rich in phenolic compounds obtained from Stevia rebaudiana (Bert.). Bertoni leaves.

    Science.gov (United States)

    Milani, Paula Gimenez; Formigoni, Maysa; Lima, Yago Carvalho; Piovan, Silvano; Peixoto, Giuliana Maria Ledesma; Camparsi, Daiane Montoia; da Silva Rodrigues, Willian do Nascimento; da Silva, Jordana Quaglia Pereira; da Silva Avincola, Alexandre; Pilau, Eduardo Jorge; da Costa, Cecília Edna Mareze; da Costa, Silvio Cláudio

    2017-06-01

    A stevia fraction (ASF) free of steviol glycosides was extracted from Stevia rebaudiana leaves (Stevia UEM-13). ASF essentially constitutes phenolic compounds (52.42%), which were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS) as caffeic acid, quercetin-3-o-glycoside, cyanidin-3-glucoside, kaempferol, quercetin, apigenin, rozmarinic acid, chlorogenic acid and dicaffeoylquinic acid. ASF was used as a multi-functional source of phenolic compounds to fortify the whey protein isolate (WPI) obtained by membrane separation. WPI fortified with 0.2% ASF showed an 80% increase in its antioxidant activity and more pronounced antidiabetic effects than the unfortified WPI, mainly in the glycemic control of diabetic animals induced by streptozotocin. The in vitro and in vivo antioxidant effects of ASF may enhance the effects of WPI. Indeed, this pioneering study revealed that ASF can be used to enrich the antioxidant and antidiabetic properties of WPI.

  17. Estudio de la Stevia (Stevia rebaudiana Bertoni) como edulcorante natural y su uso en beneficio de la salud

    OpenAIRE

    Rebeca Salvador-Reyes; Medali Sotelo-Herrera; Luz Paucar-Menacho

    2014-01-01

    Stevia, also known as "The miracle sweetener", is considered the best sugar substitute because it is up to 300 times sweeter and contains no calories. This shrub, grown in the forests of Brazil and Paraguay, has in its composition a high percentage of steviol glycosides (stevioside and rebaudioside A), which give it an intensely sweet taste and therapeutic properties against diabetes, hypertension and obesity also helps control weight, satiety and hunger. For its content in phenolic compounds...

  18. Intensified Separation of Steviol Glycosides from a Crude Aqueous Extract of Stevia rebaudiana Leaves Using Centrifugal Partition Chromatography.

    Science.gov (United States)

    Hubert, Jane; Borie, Nicolas; Chollet, Sébastien; Perret, Joël; Barbet-Massin, Claire; Berger, Monique; Daydé, Jean; Renault, Jean-Hugues

    2015-11-01

    Aqueous extracts of Stevia rebaudiana leaves have been approved since 2008 by the Joint Expert Committee for Food Additives as sugar substitutes in many food and beverages in Western and Far East Asian countries. The compounds responsible for the natural sweetness of Stevia leaves include a diversity of diterpenoid glycosides derived from a steviol skeleton. These steviol glycosides also exhibit a low calorific value as well as promising therapeutic applications, particularly for the treatment of sugar metabolism disturbances. In this work, centrifugal partition chromatography is proposed as an efficient technical alternative to purify steviol glycosides from crude aqueous extracts of Stevia leaves on a multigram scale. Two different commercial instruments, including an ASCPC250® and a FCPE300® made of columns containing 1890 and 231 twin-cells, respectively, were evaluated and compared. All experiments were performed with a polar biphasic solvent system composed of ethyl acetate, n-butanol, and water in a gradient elution mode. When using the 1890 partition cell centrifugal partition chromatography column of 250 mL, 42 mg of stevioside, 68 mg of dulcoside A, and 172 mg of rebaudioside A, three major constituents of the initial extract were obtained from 1 g of the initial mixture at purities of 81%, 83%, and 99%, respectively. The productivity was further improved by intensifying the procedure on the 231 partition cell centrifugal partition chromatography column of 303 mL with the sample mass loading increased up to 5 g, resulting in the recovery of 1.2 g of stevioside, 100 mg of dulcoside A, and 1.1 g of rebaudioside A at purities of 79%, 62%, and 98%, respectively. The structures of the isolated compounds were validated by HPLC-UV, ESI-MS, (1)H, and (13)C NMR analyses. Altogether, the results demonstrate that the column design (i.e., the partition cell number) is an important aspect to be considered for a larger scale centrifugal partition chromatography

  19. Interaction Between Phosphorus and Zinc on the Biomass Yield and Yield Attributes of the Medicinal Plant Stevia (Stevia rebaudiana

    Directory of Open Access Journals (Sweden)

    Kuntal Das

    2005-01-01

    Full Text Available A greenhouse experiment was conducted at the Indian Institute of Horticultural Research (IIHR, Bangalore to study the interaction effect between phosphorus (P and zinc (Zn on the yield and yield attributes of the medicinal plant stevia. The results show that the yield and yield attributes have been found to be significantly affected by different treatments. The total yield in terms of biomass production has been increased significantly with the application of Zn and P in different combinations and methods, being highest (23.34 g fresh biomass in the treatment where Zn was applied as both soil (10 kg ZnSO4/ha and foliar spray (0.2% ZnSO4. The results also envisaged that the different yield attributes viz. height, total number of branches, and number of leaves per plant have been found to be varied with treatments, being highest in the treatment where Zn was applied as both soil and foliar spray without the application of P. The results further indicated that the yield and yield attributes of stevia have been found to be decreased in the treatment where Zn was applied as both soil and foliar spray along with P suggesting an antagonistic effect between Zn and P.

  20. Aspectos nutricionales y metabolismo de Stevia rebaudiana (Bertoni. Una revisión

    Directory of Open Access Journals (Sweden)

    Jarma Alfredo de Jesus

    2010-08-01

    Full Text Available Estevia es una planta selvática subtropical del alto Paraná,
    nativa del Noroeste de la provincia de Misiones en el Paraguay.
    Posee un potente edulcorante que llega a ser hasta 300 veces
    más dulce que la sacarosa y no contiene calorías. Las moléculas
    responsables de esta característica son glucósidos de diterpeno
    que se encuentran en las hojas y, cuando menos en los estadios
    iniciales, son sintetizados a partir del mevalonato, a través de
    la misma ruta del ácido giberélico. Los estudios que abordan
    el tema de la nutrición mineral en estevia en el mundo son
    escasos y se circunscriben en gran parte, a las investigaciones
    adelantadas en el Brasil por Malavolta y sus colaboradores en
    1997. En Colombia, los estudios científicos sobre el tema se
    limitan a algunos resultados encontrados por la Universidad de
    Córdoba y Corpoica en trabajos desarrollados con nitrógeno,
    fósforo y potasio. Por tal motivo, el presente trabajo revisa la
    ruta de síntesis de los principales edulcorantes producidos por
    la planta, discute el importante papel que en ella juegan algunos
    minerales, y realiza un análisis de los aspectos nutricionales más
    importantes de esta planta, con base en los estudios realizados
    en Brasil y Colombia.

  1. Glucose concentration in the blood of intact and alloxan-treated mice after pretreatment with commercial preparations of Stevia rebaudiana (Bertoni).

    Science.gov (United States)

    Raskovic, Aleksandar; Gavrilovic, Maja; Jakovljevic, Vida; Sabo, Jan

    2004-01-01

    The study was concerned with the effect of mice pretreatment with two commercial products of Stevia rebaudiana Bertoni on the blood glucose concentration. One group of mice was pretreated four days with 200 mg/kg of Stevita (Stevita Co, INC, Arlington Texas) (stevia) and the other with 20 mg/kg of Clear Steviosides liquid (Stevita Co, INC, Herbal supplement, Brazil) (stevioside), whereas the animals of control group received at the same time physiological solution. Blood glucose concentration was measured before pretreatment and four days after that. The changes in glucose level were provoked by glucose-tolerance test (500 mg/kg, p.o.) and subcutaneous injection of adrenaline (0.2 mg/kg). The same procedure of measuring blood glucose was applied on the mice with alloxan-induced diabetes mellitus (two doses of 100 mg/kg with a 24-hour interval). Blood glucose levels in mice pretreated with stevia and stevioside were lower compared with control (7.82:6.82:8.01). Also, a smaller increase in this parameter compared to control was registered with pretreated mice in the glucose-tolerance test, pretreatment with stevioside being again more effective (8.68:6.36:5.82). Pretreatment with stevioside caused no significant increase in blood glucose concentration after administering adrenaline, which was not the case with the animals pretreated with stevia and control. Pretreatment with stevia, and to a greater extent with stevioside, protected test animals from the toxic action of alloxan compared with controls.

  2. Effects of growth stage and fulvic acid on the diversity and dynamics of endophytic bacterial community in Stevia rebaudiana Bertoni leaves

    Directory of Open Access Journals (Sweden)

    Xuejian eYu

    2015-08-01

    Full Text Available The aim of this study was to learn the interactions among the endophytic bacteria, the plant growth, the foliar spray of fulvic acid, and the accumulation of steviol glycosides in the leaves of Stevia rebaudiana. Metagenomic DNA was extracted from the Stevia leaves at different growth stages with or without the fulvic acid treatment; and the diversity of endophytic bacteria in Stevia leaves was estimated by pyrosequencing of 16S rRNA genes. As results, Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes were found to be the dominant phyla despite the growth stages and fulvic acid application. Stevia growth stages strongly regulated composition of endophytic community. The genera Agrobacterium (12.3 % and Erwinia (7.2 % dominated in seedling stage were apparently declined in the vegetable and initial flowering stages, while Sphingomonas and Methylobacterium increased in mature leaves at harvest time, which showed that the mature leaves of Stevia preferred to accumulate some certain endophytic bacteria. Sphingomonas and Methylobacterium constituted an important part of the core endophytic community and were positively correlated with the stevioside content and UGT74G1 gene expression, respectively; while Erwinia, Agrobacterium and Bacillus were negatively correlated with the stevioside accumulation. Fulvic acid treatment accelerated the variation of endophytes along the growth stages.

  3. Integration of the sensory experience and post-ingestive measures for understanding food satisfaction. A case study on sucrose replacement by Stevia rebaudiana and addition of beta glucan in fruit drinks

    DEFF Research Database (Denmark)

    Andersen, Barbara Vad; Mielby, Line H.; Viemose, Ida

    2017-01-01

    apple-cherry fruit drinks with different levels of beta-glucans and different sweeteners, sucrose or Stevia rebaudiana. The aims were: 1) to study the hedonic sensory experience, 2) to study time and product effects on post-ingestive sensations and satisfaction, and 3) to study main drivers....... Satisfaction with sensory attributes was found to be the main driver of food satisfaction, while post-ingestive sensations drove satisfaction as well. While replacing sucrose with Stevia rebaudiana did not affect the hedonic and post-ingestive sensations, addition of beta glucan resulted in both positive...

  4. Environmental cues induce changes of steviol glycosides contents and transcription of corresponding biosynthetic genes in Stevia rebaudiana.

    Science.gov (United States)

    Yang, Yongheng; Huang, Suzhen; Han, Yulin; Yuan, Haiyan; Gu, Chunsun; Wang, Zhongwei

    2015-01-01

    Plant growth and secondary metabolism are commonly regulated by external cues such as light, temperature and water availability. In this study, the influences of low and high temperatures, dehydration, photoperiods, and different growing stages on the changes of steviol glycosides (SGs) contents and transcription levels of fifteen genes involved in SGs biosynthesis of Stevia rebaudiana Bertoni were examined using HPLC and RT-PCR. The observations showed that the transcript levels of all the fifteen genes were maximum under 25 °C treatment, and the transcription of SrDXS, SrDXR, SrMCT, SrCMK, SrMDS, SrHDS, SrHDR, SrIDI, SrGGDPS, SrCPPS1, SrUGT85C2 and SrUGT76G1 were restrained both in low temperature (15 °C) and high temperature (35 °C). Most genes in SGs biosynthesis pathway exhibited down-regulation in dehydration. To elucidate the effect of photoperiods, the plants were treated by different simulated photoperiods (8 L/16 D, 1 0L/14 D, 14 L/10 D and 16 L/8 D), but no significant transcription changes were observed. In the study of growing stages, there were evident changes of SGs contents, and the transcript levels of all the fifteen genes were minimal in fast growing period, and exhibited evident increase both in flower-bud appearing stage and flowering stage. The obtained results strongly suggest that the effect of environmental cues on steviol glycosides contents and transcription of corresponding biosynthetic genes in S. rebaudiana is significant. It is worth to study deeply. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  5. Validation of HPLC-UV method for determination of minor glycosides contained in Stevia rebaudiana Bertoni leaves.

    Science.gov (United States)

    Aranda-González, Irma; Moguel-Ordoñez, Yolanda; Betancur-Ancona, David

    2015-05-01

    Leaves of Stevia rebaudiana contain glycosides with sweetness and biological activity. However besides the major glycosides, there are other glycosides within extracts that may contribute to its activity, and therefore it is important to quantify them. In this work, an isocratic HPLC method was validated for determination of dulcoside A, steviolbioside, rebaudioside C and rebaudioside B. An HPLC method was performed using a C18 column (250 × 4.6 mm, particle size 5 µm) and a UV detector set at 210 nm. The mobile phase consisted of a 32:68 (v/v) mixture of acetonitrile and sodium phosphate buffer (10 mmol/L, pH 2.6), set to a flow rate of 1.0 mL/min. The calculated parameters were: sensitivity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy and precision. The calibration curves were linear over the working range 25-150 µg/mL, with coefficient of correlation of ≥0.99 and coefficient of determination of ≥0.98. The LOD was 5.68-8.81 µg/mL, while the LOQ was 17.21-26.69 µg/mL. The percentage recoveries of fortified samples were 100 ± 10% and precision, relative standard deviation, was <10%. The method validation showed accuracy, linearity and precision; therefore this method can be applied for quantitative analysis of minor steviol glycosides in S. rebaudiana leaves. Copyright © 2014 John Wiley & Sons, Ltd.

  6. Análise tempo-intensidade dos estímulos doce e amargo de extrato de folhas de estévia [Stevia rebaudiana (Bert. Bertoni] em doçura equivalente a sacarose

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    CARDELLO Helena Maria André Bolini

    1999-01-01

    Full Text Available O extrato de folhas de estévia (Stevia rebaudiana Bertoni é o único edulcorante utilizado na substituição da sacarose, que pode ser produzido totalmente no Brasil. O objetivo deste estudo foi determinar os comportamentos de características temporais dos estímulos doce e amargo da estevia em doçuras equivalentes a soluções de sacarose (DESS a 3 e 10%. As curvas tempo-intensidade (T-I para cada substância foram coletadas utilizando-se o programa "Sistema de Coleta de Dados Tempo-Intensidade - SCDTI" para Windows, onde os provadores registravam com auxílio do "mouse" a percepção de cada estímulo solicitado em função do tempo, para cada amostra. Os parâmetros das curvas T-I coletados foram: tempo de intensidade máxima (TImax, intensidade máxima (Imax, tempo onde a intensidade máxima começa a declinar (Td, tempo de platô (Platô, área sob a curva (Área e tempo total de duração do estímulo (Ttot. Os parâmetros Td, Ttot, Área e Platô das curvas T-I para o estímulo doce nos dois níveis de doçura foram significativamente superiores para estévia, enquanto TImax e Imax foram significativamente menores (p£0,05, sendo que as diferenças entre os valores para as duas substâncias foram muito maiores a DESS a 10%. A sacarose não apresentou nenhum registro para o estímulo amargo tanto a 3 como a 10%, enquanto a estévia apresentou curvas T-I características, com intensidade e o tempo total de duração dependente da concentração.

  7. Biomass Yield and Steviol Glycoside Production in Callus and Suspension Culture of Stevia rebaudiana Treated with Proline and Polyethylene Glycol.

    Science.gov (United States)

    Gupta, Pratibha; Sharma, Satyawati; Saxena, Sanjay

    2015-06-01

    Enhanced production of steviol glycosides (SGs) was observed in callus and suspension culture of Stevia rebaudiana treated with proline and polyethylene glycol (PEG). To study their effect, yellow-green and compact calli obtained from in vitro raised Stevia leaves were sub-cultured on MS medium supplemented with 2.0 mg l(-1) NAA and different concentrations of proline (2.5-10 mM) and PEG (2.5-10 %) for 2 weeks, and incubated at 24 ± 1 °C and 22.4 μmol m(-2) s(-1) light intensity provided by white fluorescent tubes for 16 h. Callus and suspension culture biomass (i.e. both fresh and dry weight content) was increased with 5 mM proline and 5 % PEG, while at further higher concentrations, they got reduced. Further, quantification of SGs content in callus (collected at 15th day) and suspension culture (collected at 10th and 15th day) treated with and without elicitors was analysed by HPLC. It was observed that chemical stress enhanced the production of SGs significantly. In callus, the content of SGs increased from 0.27 (control) to 1.09 and 1.83 % with 7.5 mM proline and 5 % PEG, respectively, which was about 4.0 and 7.0 times higher than control. However, in the case of suspension culture, the same concentrations of proline and polyethylene glycol enhanced the SG content from 1.36 (control) to 5.03 and 6.38 %, respectively, on 10th day which were 3.7 times and 4.7 times higher than control.

  8. Effects of growth stage and fulvic acid on the diversity and dynamics of endophytic bacterial community in Stevia rebaudiana Bertoni leaves

    Science.gov (United States)

    Yu, Xuejian; Yang, Jinshui; Wang, Entao; Li, Baozhen; Yuan, Hongli

    2015-01-01

    The aim of this study was to learn the interactions among the endophytic bacteria, the plant growth, the foliar spray of fulvic acid, and the accumulation of steviol glycosides in the leaves of Stevia rebaudiana. Metagenomic DNA was extracted from the Stevia leaves at different growth stages with or without the fulvic acid treatment; and the diversity of endophytic bacteria in Stevia leaves was estimated by pyrosequencing of 16S rRNA genes. As results, Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes were found to be the dominant phyla despite the growth stages and fulvic acid application. Stevia growth stages strongly regulated composition of endophytic community. The genera Agrobacterium (12.3%) and Erwinia (7.2%) dominated in seedling stage were apparently declined in the vegetable and initial flowering stages, while Sphingomonas and Methylobacterium increased in mature leaves at harvest time, which showed that the mature leaves of Stevia preferred to accumulate some certain endophytic bacteria. Sphingomonas and Methylobacterium constituted an important part of the core endophytic community and were positively correlated with the stevioside content and UGT74G1 gene expression, respectively; while Erwinia, Agrobacterium, and Bacillus were negatively correlated with the stevioside accumulation. Fulvic acid treatment accelerated the variation of endophytes along the growth stages and increased the steviol glycosides content. This is the first study to reveal the community composition of endophytic bacteria in the Stevia leaves, to evidence the strong effects of growth stage and fulvic acid application on the endophytes of Stevia, and to demonstrate the correlation between the endophytic bacteria and the steviol glycosides accumulation. PMID:26379644

  9. Effects of growth stage and fulvic acid on the diversity and dynamics of endophytic bacterial community in Stevia rebaudiana Bertoni leaves.

    Science.gov (United States)

    Yu, Xuejian; Yang, Jinshui; Wang, Entao; Li, Baozhen; Yuan, Hongli

    2015-01-01

    The aim of this study was to learn the interactions among the endophytic bacteria, the plant growth, the foliar spray of fulvic acid, and the accumulation of steviol glycosides in the leaves of Stevia rebaudiana. Metagenomic DNA was extracted from the Stevia leaves at different growth stages with or without the fulvic acid treatment; and the diversity of endophytic bacteria in Stevia leaves was estimated by pyrosequencing of 16S rRNA genes. As results, Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes were found to be the dominant phyla despite the growth stages and fulvic acid application. Stevia growth stages strongly regulated composition of endophytic community. The genera Agrobacterium (12.3%) and Erwinia (7.2%) dominated in seedling stage were apparently declined in the vegetable and initial flowering stages, while Sphingomonas and Methylobacterium increased in mature leaves at harvest time, which showed that the mature leaves of Stevia preferred to accumulate some certain endophytic bacteria. Sphingomonas and Methylobacterium constituted an important part of the core endophytic community and were positively correlated with the stevioside content and UGT74G1 gene expression, respectively; while Erwinia, Agrobacterium, and Bacillus were negatively correlated with the stevioside accumulation. Fulvic acid treatment accelerated the variation of endophytes along the growth stages and increased the steviol glycosides content. This is the first study to reveal the community composition of endophytic bacteria in the Stevia leaves, to evidence the strong effects of growth stage and fulvic acid application on the endophytes of Stevia, and to demonstrate the correlation between the endophytic bacteria and the steviol glycosides accumulation.

  10. Antidiabetic activity of medium-polar extract from the leaves of Stevia rebaudiana Bert. (Bertoni) on alloxan-induced diabetic rats.

    Science.gov (United States)

    Misra, Himanshu; Soni, Manish; Silawat, Narendra; Mehta, Darshana; Mehta, B K; Jain, D C

    2011-04-01

    To investigate the medicative effects of medium-polar (benzene:acetone, 1:1, v/v) extract of leaves from Stevia rebaudiana (family Asteraceae) on alloxan-induced diabetic rats. Diabetes was induced in adult albino Wistar rats by intraperitoneal (i.p.) injection of alloxan (180 mg/kg). Medium-polar extract was administered orally at daily dose of 200 and 400 mg/kg body wt. basis for 10 days. The control group received normal saline (0.9%) for the same duration. Glibenclamide was used as positive control reference drug against Stevia extract. Medium-polar leaf extract of S. rebaudiana (200 and 400 mg/kg) produced a delayed but significant (P diabetes with sulfonylurea drugs (glibenclamide) causes hypoglycemia followed by greater reduction in body weight, which are the most worrisome effects of these drugs. Stevia extract was found to antagonize the necrotic action of alloxan and thus had a re-vitalizing effect on β-cells of pancreas.

  11. Antidiabetic activity of medium-polar extract from the leaves of Stevia rebaudiana Bert. (Bertoni on alloxan-induced diabetic rats

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    Himanshu Misra

    2011-01-01

    Full Text Available Objective: To investigate the medicative effects of medium-polar (benzene:acetone, 1:1, v/v extract of leaves from Stevia rebaudiana (family Asteraceae on alloxan-induced diabetic rats. Materials and Methods: Diabetes was induced in adult albino Wistar rats by intraperitoneal (i.p. injection of alloxan (180 mg/kg. Medium-polar extract was administered orally at daily dose of 200 and 400 mg/kg body wt. basis for 10 days. The control group received normal saline (0.9% for the same duration. Glibenclamide was used as positive control reference drug against Stevia extract. Results: Medium-polar leaf extract of S. rebaudiana (200 and 400 mg/kg produced a delayed but significant (P < 0.01 decrease in the blood glucose level, without producing condition of hypoglycemia after treatment, together with lesser loss in the body weight as compared with standard positive control drug glibenclamide. Conclusions: Treatment of diabetes with sulfonylurea drugs (glibenclamide causes hypoglycemia followed by greater reduction in body weight, which are the most worrisome effects of these drugs. Stevia extract was found to antagonize the necrotic action of alloxan and thus had a re-vitalizing effect on β-cells of pancreas.

  12. Stevia rebaudiana Leaves: Effect of Drying Process Temperature on Bioactive Components, Antioxidant Capacity and Natural Sweeteners.

    Science.gov (United States)

    Lemus-Mondaca, Roberto; Ah-Hen, Kong; Vega-Gálvez, Antonio; Honores, Carolina; Moraga, Nelson O

    2016-03-01

    Stevia leaves are usually used in dried state and undergo the inevitable effect of drying process that changes the quality characteristics of the final product. The aim of this study was to assess temperature effect on Stevia leaves through analysis of relevant bioactive components, antioxidant capacity and content of natural sweeteners and minerals. The drying process was performed in a convective dryer at constant temperatures ranging from 30 to 80 °C. Vitamin C was determined in the leaves and as expected showed a decrease during drying proportional to temperature. Phenolics and flavonoids were also determined and were found to increase during drying below 50 °C. Antioxidant activity was determined by DPPH and ORAC assays, and the latter showed the highest value at 40 °C, with a better correlation with the phenolics and flavonoids content. The content of eight natural sweeteners found in Stevia leaves was also determined and an increase in the content of seven of the sweeteners, excluding steviol bioside, was found at drying temperature up to 50 °C. At temperatures between 60 and 80 °C the increase in sweeteners content was not significant. Stevia leaves proved to be an excellent source of antioxidants and natural sweeteners.

  13. Radiosensitivity of the in vitro cultured young plants for sport mutation induction of stevia rebaudiana bert

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Tai Young; Kim, Ee Youb; Hyun, Kyung Sup; Jo, Han Jig; Lee, Young Il; Ju, Sun Ah; Oh, Seung Cheol [Korea Stevia Research Institute, Jeongeup (Korea, Republic of); Kim, Dong Sub; Kang, Si Yong [Korea Atomic Energy Research Institute, Jeongeup (Korea, Republic of); Ko, Jeong Ae [Chonbuk National University, Jeonju (Korea, Republic of)

    2010-12-15

    Due to the increasing incidence of diabetes, obesity and hypertensive, stevia has been placed great attentions as the sweetener to substitute sucrose in the world. Stevia was introduced to Korea in 1970's, but it has not been an attractive crop in that time. However, recently it has more attention for the natural food sweet additives. Because stevia have many problems for cultivation especially cultivar, seed germination, fertility, uniformity and glycoside quality, the sport mutation was attempted to in vitro plants for the improvement of some characteristics. The young in vitro plants was nursed on MS medium supplemented with 1 mg 1{sup -1} GA{sub 3}. Shoots of 10 cm height were irradiated with 0 {approx} 200 Gy of gamma ray and the every node was separated and inoculated on MS basic medium. The lethality, number and length of shoot, numbers of node and branch were investigated for the evaluation of radiosensitivity. The optimum dose of gamma ray seemed to be around 80 Gy for the sport mutation induction in stevia. The lower node was more sensitive than higher node to radiation.

  14. Bioaccessibility of bioactive compounds after non-thermal processing of an exotic fruit juice blend sweetened with Stevia rebaudiana.

    Science.gov (United States)

    Buniowska, Magdalena; Carbonell-Capella, Juana M; Frigola, Ana; Esteve, Maria J

    2017-04-15

    A comparative study of the bioaccessibility of bioactive compounds and antioxidant capacity in a fruit juice-Stevia rebaudiana mixture processed by pulsed electric fields (PEF), high voltage electrical discharges (HVED) and ultrasound (USN) technology at two equivalent energy inputs (32-256kJ/kg) was made using an in vitro model. Ascorbic acid was not detected following intestinal digestion, while HVED, PEF and USN treatments increased total carotenoid bioaccessibility. HVED at an energy input of 32kJ/kg improved bioaccessibility of phenolic compounds (34.2%), anthocyanins (31.0%) and antioxidant capacity (35.8%, 29.1%, 31.9%, for TEAC, ORAC and DPPH assay, respectively) compared to untreated sample. This was also observed for PEF treated samples at an energy input of 256kJ/kg (37.0%, 15.6%, 29.4%, 26.5%, 23.5% for phenolics, anthocyanins, and antioxidant capacity using TEAC, ORAC and DPPH method, respectively). Consequently, pulsed electric technologies (HVED and PEF) show good prospects for enhanced bioaccessibility of compounds with putative health benefit. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. Clarificação do extrato aquoso de Stevia rebaudiana (Bert. Bertoni utilizando o cacto, Cereus peruvianus

    Directory of Open Access Journals (Sweden)

    Larissa Maria Fernandes

    2001-05-01

    Full Text Available A tendência das indústrias que se preocupam em promover a saúde e prevenir doenças está em investir no desenvolvimento de tecnologias para produção de alimentos com baixas calorias e reduzido teor de gordura, mantendo suas qualidades nutricionais. Um dos estudos mais freqüentes é a substituição do açúcar por edulcorantes não calóricos, com a preocupação que estes substitutos sejam semelhantes em função organoléptica. Nas folhas da planta conhecida como Stevia rebaudiana (Bert. Bertoni (Asteraceae, ou simplesmente estévia, se encontram glicosídeos adoçantes (edulcorantes, como o esteviosídeo, rebaudiosídeos A, B, C, D, E e dulcosídeos A e B. Seu extrato aquoso apresenta-se em estado natural na cor marrom, o que o torna inconveniente para o consumidor usual. Neste trabalho, utilizou-se o cacto Cereus peruvianus como alternativa natural para a clarificação deste extrato. O cacto comportou-se como uma resina fortemente ácida e eliminou cerca de 95%, em média, a cor e turbidez do extrato de estévia

  16. Microencapsulation of steviol glycosides (Stevia rebaudiana Bertoni by a spray drying method – Evaluation of encapsulated products and prepared syrups

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    Charikleia Chranioti

    2015-10-01

    Full Text Available The aim of this study was to encapsulate the steviol glycosides (SGs, derived from Stevia rebaudiana (Bert Bertoni leaves, by applying a spray-drying method. The purpose was to minimize the bitter aftertaste of the SGs as well as to ameliorate/improve their properties. The encapsulation agents used were maltodextrin (19 DE and inulin in a ratio of 80 : 20, while three levels of SGs in total solids (1.5, 2.5 and 3.5 % were studied. The encapsulated SGs products were evaluated for their hygroscopicity, solubility, moisture content and microencapsulation efficiency (MEE %. Also, syrups prepared with encapsulated SGs, at 1 % w/v, were tested for their viscosity, refractive index, turbidity and sensory properties. Significant differences (P<0.05  in MEE %, moisture content, hygroscopicity and solubility values of the encapsulated SGs products were observed depending on the level of SGs in total solids. In particular, the MEE %, the hygroscopicity and the solubility values ranged from 62.36, 82.46 to 94.67 %, 21.51, 26.67 and 24.25 % and 99.93, 97.50 to 96.03 % for encapsulated SGs products produced with 1.5, 2.5 and 3.5 % SGs in total solids, respectively. The encapsulated product produced with 2.5 % steviol glycosides in total solids presented the most appealing sensory and quality characteristics.

  17. Estudio de la Stevia (Stevia rebaudiana Bertoni como edulcorante natural y su uso en beneficio de la salud

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    Rebeca Salvador-Reyes

    2014-01-01

    Full Text Available Lastevia, conocida también como “El edulcorante milagroso”, es considerada el mejor sustituto del azúcardebido a que es hasta 300 veces más dulce y no contiene calorías. Este arbusto, cultivado en los bosques deBrasil y Paraguay, presenta en su composición unalto porcentaje de glucósidos de esteviol (esteviósido yrebaudiosida A, los cuales le confieren un sabor dulce intenso y propiedades terapéuticas contra la diabetes, lahipertensión y la obesidad; además ayuda al control del peso, la saciedad y el hambre. Por su contenido encompuestos fenólicos, lastevia actúa también como un excelente antioxidante y anticancerígeno; asimismo seha demostrado que posee propiedades antibacterianas, anticonceptivas y diuréticas. Este trabajo de revisióntuvo como objetivo, recopilar información sobre los estudios realizados a la stevia como alternativa al azúcar ylos beneficios de su consumo.

  18. Catalytic Hydrogenation of the Sweet Principles of Stevia rebaudiana, Rebaudioside B, Rebaudioside C, and Rebaudioside D and Sensory Evaluation of Their Reduced Derivatives

    Science.gov (United States)

    Prakash, Indra; Campbell, Mary; Chaturvedula, Venkata Sai Prakash

    2012-01-01

    Catalytic hydrogenation of rebaudioside B, rebaudioside C, and rebaudioside D; the three ent-kaurane diterpene glycosides isolated from Stevia rebaudiana was carried out using Pd(OH)2. Reduction of steviol glycosides was performed using straightforward synthetic chemistry with the catalyst Pd(OH)2 and structures of the corresponding dihydro derivatives were characterized on the basis of 1D and 2D nuclear magnetic resonance (NMR) spectral data indicating that all are novel compounds being reported for the first time. Also, the taste properties of all reduced compounds were evaluated against their corresponding original steviol glycosides and sucrose. PMID:23203115

  19. Chloroplast ultra structure, photosynthesis and enzyme activities in regenerated plants of Stevia rebaudiana (Bert.) Bertoni as influenced by copper sulphate in the medium.

    Science.gov (United States)

    Jain, Pourvi; Kachhwaha, Sumita; Kothari, S L

    2014-09-01

    Stevia rebaudiana (Bert.) Bertoni is an important medicinal plant used as noncaloric commercial sweetener. Plants regenerated with higher levels of copper sulphate in the medium exhibited enhanced activity of peroxidase and polyphenoloxidase (PPO) enzymes. Transmission electron microscopy (TEM) revealed increase in size and number of electron dense inclusions in the chloroplasts of plants regenerated at optimised level of copper sulphate (0.5 microM) in the medium. There was decrease in chlorogenic acid (CGA) content. Chl-a-fluorescence transient pattern (OJIP) showed that the photosynthesis process was more efficient at 0.5 microM CuSO4 in the medium.

  20. Catalytic Hydrogenation of the Sweet Principles of Stevia rebaudiana, Rebaudioside B, Rebaudioside C, and Rebaudioside D and Sensory Evaluation of Their Reduced Derivatives

    Directory of Open Access Journals (Sweden)

    Mary Campbell

    2012-11-01

    Full Text Available Catalytic hydrogenation of rebaudioside B, rebaudioside C, and rebaudioside D; the three ent-kaurane diterpene glycosides isolated from Stevia rebaudiana was carried out using Pd(OH2. Reduction of steviol glycosides was performed using straightforward synthetic chemistry with the catalyst Pd(OH2 and structures of the corresponding dihydro derivatives were characterized on the basis of 1D and 2D nuclear magnetic resonance (NMR spectral data indicating that all are novel compounds being reported for the first time. Also, the taste properties of all reduced compounds were evaluated against their corresponding original steviol glycosides and sucrose.

  1. Transcript Quantification of Genes Involved in Steviol Glycoside Biosynthesis in Stevia rebaudiana Bertoni by Real-Time Polymerase Chain Reaction (RT-PCR).

    Science.gov (United States)

    Modi, Arpan; Kumar, Nitish; Narayanan, Subhash

    2016-01-01

    Stevia (Stevia rebaudiana Bertoni) is a medicinal plant having sweet, diterpenoid glycosides known as steviol glycosides which are 200-300 times sweeter than sucrose (0.4 % solution). They are synthesized mainly in the leaves via plastid localized 2-C-methyl-D-erythrose-4-phosphate pathway (MEP pathway). Fifteen genes are involved in the formation of these glycosides. In the present protocol, a method for the quantification of transcripts of these genes is shown. The work involves RNA extraction and cDNA preparation, and therefore, procedures for the confirmation of DNA-free cDNA preparation have also been illustrated. Moreover, details of plant treatments are not mentioned as this protocol may apply to relative gene expression profile in any medicinal plant with any treatment. The treatments are numbered as T0 (Control), T1, T2, T3, and T4.

  2. Response surface methodology to optimise Accelerated Solvent Extraction of steviol glycosides from Stevia rebaudiana Bertoni leaves.

    Science.gov (United States)

    Jentzer, Jean-Baptiste; Alignan, Marion; Vaca-Garcia, Carlos; Rigal, Luc; Vilarem, Gérard

    2015-01-01

    Following the approval of steviol glycosides as a food additive in Europe in December 2011, large-scale stevia cultivation will have to be developed within the EU. Thus there is a need to increase the efficiency of stevia evaluation through germplasm enhancement and agronomic improvement programs. To address the need for faster and reproducible sample throughput, conditions for automated extraction of dried stevia leaves using Accelerated Solvent Extraction were optimised. A response surface methodology was used to investigate the influence of three factors: extraction temperature, static time and cycle number on the stevioside and rebaudioside A extraction yields. The model showed that all the factors had an individual influence on the yield. Optimum extraction conditions were set at 100 °C, 4 min and 1 cycle, which yielded 91.8% ± 3.4% of total extractable steviol glycosides analysed. An additional optimisation was achieved by reducing the grind size of the leaves giving a final yield of 100.8% ± 3.3%. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. Influence of drying method on steviol glycosides and antioxidants in Stevia rebaudiana leaves.

    Science.gov (United States)

    Periche, Angela; Castelló, María Luisa; Heredia, Ana; Escriche, Isabel

    2015-04-01

    The application of different drying conditions (hot air drying at 100 °C and 180 °C, freeze drying and shade drying) on steviol glycosides (stevioside, dulcoside A, rebaudioside A and rebaudioside C) and antioxidants in Stevia leaves was evaluated. Stevioside, the major glycoside found in fresh leaves (81.2mg/g), suffered an important reduction in all cases, although shade drying was the least aggressive treatment. Considering the antioxidant parameters (total phenols, flavonoids and total antioxidants), the most suitable drying method was hot air at 180 °C, since it substantially increased all of them (76.8 mg gallic acid, 45.1mg catechin and 126 mg Trolox, all equivalent/g Stevia, respectively), with respect to those present in fresh leaves (44.4, 2.5 and 52.9 mg equivalent/g). Therefore, the ideal method for drying Stevia leaves depends on their final use (sweetener or antioxidant), although, hot air at 180 °C is the most recommendable if only one treatment has to be chosen. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. Stevia rebaudiana (Bert.) Bertoni incorporação em uma receita de bolo

    OpenAIRE

    Oliveira, Adriana Isabel Miranda de

    2016-01-01

    Trabalho Complementar apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de licenciada em Ciências da Nutrição Este trabalho tem como objetivo avaliar o desenvolvimento e aceitação de preparações de diferentes bolos de limão com integração da Stevia como adoçante natural em receitas caseiras destinadas a diabéticos e obesos. Foram realizadas quatro formulações, sendo um bolo controlo (receita tradicional com açúcar), dois bolos com incorpora...

  5. Yield, Quality and Water Consumption of Stevia rebaudiana Bertoni Grown under Different Irrigation Regimes in Southern Italy

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    Riccardo d’Andria

    2008-06-01

    Full Text Available Stevia rebaudiana Bertoni is a herbaceous perennial plant originating in the north-east of Paraguay. Its leaves contain low-calorie sweetening agents that can be used as a natural alternative to artificial sweeteners. The leaves are consumed in special human diets and for the treatment of various diseases. The aim of the present work is to study water consumption, yield potential and quality characteristics of this species under different irrigation levels in southern Italy. The field work was carried out in 2006-2007. Irrigation treatments consisted of a control (T100, irrigated with 100% restitution of water consumption and two treatments that received a water depth of 33% (T33 and 66% (T66 of treatment T100. Watering volume was estimated to replenish the soil profile to field capacity for a depth of 0.40 m. The crop was harvested twice a year, and agronomic performance as well as the major cation and glycoside contents (stevioside and rebaudioside A were evaluated. Overall, the crop coefficients were similar between the two years, although in each year the second growing period showed higher values due to the higher evaporative demand of this period. Interactions of years with irrigation treatments and harvest time were not significant either for yield or yield components. In both cuts the T100 treatments achieved 40% higher leaf dry yield than T33, while T66 showed intermediate values. The harvest index and water use efficiency showed no differences between the two cuts for the same treatments, while the values of both indices decreased with the increase in irrigation regime. Stevioside, rebaudioside A and cation content in the leaves were unaffected by irrigation regime. In order to develop the field cultivation of this species, field experiments are required to prepare a cultivation protocol as well as a genetic improvement program to develop varieties that better respond to the local environment.

  6. Rebaudiosides T and U, minor C-19 xylopyranosyl and arabinopyranosyl steviol glycoside derivatives from Stevia rebaudiana (Bertoni) Bertoni.

    Science.gov (United States)

    Perera, Wilmer H; Ghiviriga, Ion; Rodenburg, Douglas L; Alves, Kamilla; Bowling, John J; Avula, Bharathi; Khan, Ikhlas A; McChesney, James D

    2017-03-01

    Two diterpene glycosides were isolated from a commercial Stevia rebaudiana leaf extract. One was found to be 13-[(2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid-(2-O-β-d-xylopyranosyl-3-O-β-d-glucopyranosyl- β-d-glucopyranosyl) ester (rebaudioside T), whereas the other was determined to be 13-[(2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid-(6-O-α-l-arabinopyranosyl-β-d-glucopyranosyl) ester (rebaudioside U). In addition, five C-19 sugar free derivatives were prepared and identified as follows: 13-[(2-O-α-l-rhamnopyranosyl-β-d-glucopyranosyl)]oxy]kaur-16-en-19-oic acid (dulcoside A 1 ); 13-[(2-O-β-d-xylopyranosy-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy]kaur-16-en-19-oic acid; 13-[(2-O-β-d-xylopyranosyl-β-d-glucopyranosyl-)oxy]kaur-16-en-19-oic acid; 13-[(2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-xylopyranosyl-)oxy]kaur-16-en-19-oic acid (rebaudioside R 1 ) and 13-[(2-O-6-deoxy-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy]kaur-16-en-19-oic acid, respectively. Chemical structures were determined by NMR experiments. HPLC analyses were also useful to differentiate different steviol-C13 sugar substituent patterns by elution position. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Yield, Quality and Water Consumption of Stevia rebaudiana Bertoni Grown under Different Irrigation Regimes in Southern Italy

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    Riccardo d’Andria

    2011-02-01

    Full Text Available Stevia rebaudiana Bertoni is a herbaceous perennial plant originating in the north-east of Paraguay. Its leaves contain low-calorie sweetening agents that can be used as a natural alternative to artificial sweeteners. The leaves are consumed in special human diets and for the treatment of various diseases. The aim of the present work is to study water consumption, yield potential and quality characteristics of this species under different irrigation levels in southern Italy. The field work was carried out in 2006-2007. Irrigation treatments consisted of a control (T100, irrigated with 100% restitution of water consumption and two treatments that received a water depth of 33% (T33 and 66% (T66 of treatment T100. Watering volume was estimated to replenish the soil profile to field capacity for a depth of 0.40 m. The crop was harvested twice a year, and agronomic performance as well as the major cation and glycoside contents (stevioside and rebaudioside A were evaluated. Overall, the crop coefficients were similar between the two years, although in each year the second growing period showed higher values due to the higher evaporative demand of this period. Interactions of years with irrigation treatments and harvest time were not significant either for yield or yield components. In both cuts the T100 treatments achieved 40% higher leaf dry yield than T33, while T66 showed intermediate values. The harvest index and water use efficiency showed no differences between the two cuts for the same treatments, while the values of both indices decreased with the increase in irrigation regime. Stevioside, rebaudioside A and cation content in the leaves were unaffected by irrigation regime. In order to develop the field cultivation of this species, field experiments are required to prepare a cultivation protocol as well as a genetic improvement program to develop varieties that better respond to the local environment.

  8. Two-stage culture procedure using thidiazuron for efficient micropropagation of Stevia rebaudiana, an anti-diabetic medicinal herb.

    Science.gov (United States)

    Singh, Pallavi; Dwivedi, Padmanabh

    2014-08-01

    Stevia rebaudiana Bertoni, member of Asteraceae family, has bio-active compounds stevioside and rebaudioside which taste about 300 times sweeter than sucrose. It regulates blood sugar, prevents hypertension and tooth decay as well as used in treatment of skin disorders having high medicinal values, and hence there is a need for generating the plant on large scale. We have developed an efficient micropropagation protocol on half strength Murashige and Skoog (MS) media, using two-stage culture procedures. Varying concentrations of cytokinins, i.e., benzylaminopurine, kinetin and thidiazuron (TDZ) were supplemented in the nutrient media to observe their effects on shoot development. All the cytokinins promoted shoot formation, however, best response was observed in the TDZ (0.5 mg/l). The shoots from selected induction medium were sub-cultured on the multiplication media. The media containing 0.01 mg/l TDZ produced maximum number of shoot (11.00 ± 0.40) with longer shoots (7.17 ± 0.16) and highest number of leaves (61.00 ± 1.29). Rooting response was best observed in one-fourth strength on MS media supplemented with indole-3-butyric acid (1.0 mg/l) and activated charcoal (50 mg/l) with (11.00 ± 0.40) number of roots. The plantlets thus obtained were hardened and transferred to the pots with soil and sand mixture, where the survival rate was 80 % after 2 months. Quantitative analysis of stevioside content in leaves of in vivo mother plant and in vitro plantlets was carried out by high performance liquid chromatography. A remarkable increase in stevioside content was noticed in the in vitro-raised plants as compared to in vivo grown plants. The protocol reported here might be useful in genetic improvement and high stevioside production.

  9. Effect of decapitation and nutrient applications on shoot branching, yield, and accumulation of secondary metabolites in leaves of Stevia rebaudiana Bertoni.

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    Pal, Probir Kumar; Prasad, Ramdeen; Pathania, Vijaylata

    2013-11-15

    The axillary buds of stevia (Stevia rebaudiana Bertoni) often remain dormant for a long time and sometimes remain dormant permanently until the plants enter into the reproductive stage. The present study was conducted to ascertain whether decapitation and foliar fertilization enhance the productivity and quality of stevia through breaking the apical dominance and increasing physiological activities. Ten treatment combinations comprising two cultural operations (non-decapitation and decapitation) and five foliar spray treatments (water spray control, KNO3 @ 5.0gL(-1), Ca(NO3)2 @ 4.06gL(-1), CuSO4·5H2O 2.0gL(-1) and (NH4)6Mo7O24 @ 1.0gL(-1)) were applied. The decapitation of apical buds of stevia increased the branches and increased dry leaf yield by 13 and 17% compared with non-decapitation during 2010 and 2011, respectively, without affecting quality. Foliar application of nutrient solutions also exerted a considerable effect on growth parameters, yield attributes and chlorophyll content, and significantly (P=0.05) higher dry leaf yield ranging from 8 to 26% over the control. Among the foliar spray treatments, KNO3 @ 5.0gL(-1) and Ca (NO3)2 4.06gL(-1) were found most effective in dry leaf yield. Thus, the decapitation of apical buds and foliar application of KNO3 and Ca (NO3)2 could enhance the productivity of stevia through improving the growth of axillary buds and physiological activities. Copyright © 2013 Elsevier GmbH. All rights reserved.

  10. Influence of Extraction Methods on the Yield of Steviol Glycosides and Antioxidants in Stevia rebaudiana Extracts.

    Science.gov (United States)

    Periche, Angela; Castelló, Maria Luisa; Heredia, Ana; Escriche, Isabel

    2015-06-01

    This study evaluated the application of ultrasound techniques and microwave energy, compared to conventional extraction methods (high temperatures at atmospheric pressure), for the solid-liquid extraction of steviol glycosides (sweeteners) and antioxidants (total phenols, flavonoids and antioxidant capacity) from dehydrated Stevia leaves. Different temperatures (from 50 to 100 °C), times (from 1 to 40 min) and microwave powers (1.98 and 3.30 W/g extract) were used. There was a great difference in the resulting yields according to the treatments applied. Steviol glycosides and antioxidants were negatively correlated; therefore, there is no single treatment suitable for obtaining the highest yield in both groups of compounds simultaneously. The greatest yield of steviol glycosides was obtained with microwave energy (3.30 W/g extract, 2 min), whereas, the conventional method (90 °C, 1 min) was the most suitable for antioxidant extraction. Consequently, the best process depends on the subsequent use (sweetener or antioxidant) of the aqueous extract of Stevia leaves.

  11. Optimization of Ultrasound Assisted Extraction of Functional Ingredients from Stevia Rebaudiana Bertoni Leaves

    Science.gov (United States)

    Šic Žlabur, Jana; Voća, Sandra; Dobričević, Nadica; Brnčić, Mladen; Dujmić, Filip; Rimac Brnčić, Suzana

    2015-04-01

    The aim of the present study was to reveal an effective extraction procedure for maximization of the yield of steviol glycosides and total phenolic compounds as well as antioxidant activity in stevia extracts. Ultrasound assisted extraction was compared with conventional solvent extraction. The examined solvents were water (100°C/24 h) and 70% ethanol (at 70°C for 30 min). Qualitative and quantitative analyses of steviol glycosides in the extracts obtained were performed using high performance liquid chromatography. Total phenolic compounds, flavonoids, and radical scavenging capacity by 2, 2-azino-di-3-ethylbenzothialozine- sulphonic acid) assay were also determined. The highest content of steviol glycosides, total phenolic compounds, and flavonoids in stevia extracts were obtained when ultrasound assisted extraction was used. The antioxidant activity of the extracts was correlated with the total amount of phenolic compounds. The results indicated that the examined sonication parameters represented as the probe diameter (7 and 22 mm) and treatment time (2, 4, 6, 8, and 10 min) significantly contributed to the yield of steviol glycosides, total phenolic compounds, and flavonoids. The optimum conditions for the maximum yield of steviol glycosides, total phenolic compounds, and flavonoids were as follows: extraction time 10 min, probe diameter 22 mm, and temperature 81.2°C.

  12. Effects of salinity and short-term elevated atmospheric CO2on the chemical equilibrium between CO2fixation and photosynthetic electron transport of Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Hussin, Sayed; Geissler, Nicole; El-Far, Mervat M M; Koyro, Hans-Werner

    2017-09-01

    The effect of water salinity on plant growth and photosynthetic traits of Stevia rebaudiana was investigated to determine its level and mechanisms of salinity tolerance. It was also attempted to assess how short-term elevated CO 2 concentration would influence the boundaries and mechanisms of its photosynthetic capacity. The plants were grown in gravel/hydroponic system under controlled greenhouse conditions and irrigated with four different salinity levels (0, 25, 50 and 100 mol m -3 NaCl). Low salinity did not significantly alter the plant fresh weight, which was substantially decreased by 67% at high salinity treatment. Salinity tolerance threshold was reached at 50 mol m -3  NaCl while C50 was between 50 and 100 mol m -3  NaCl, indicating that S. rebaudiana is a moderate salt tolerant species. Salt-induced growth reduction was apparently linked to a significant decline of about 47% in the photosynthetic rates (A net ) at high salinity treatment, leading consequently to a disequilibrium between CO 2 -assimilation and electron transport rates (indicated by enhanced ETR max /A gross ratio). Elevated atmospheric CO 2 enhanced CO 2 assimilation rates by 65% and 80% for control and high-salt-stressed plants respectively, likely due to significant increases in intercellular CO 2 concentration (indicated by enhanced C i /C a ). The priority for Stevia under elevated atmospheric CO 2 was not to save water but to maximize photosynthesis so that the PWUE was progressively improved and the threat of oxidative stress was diminished (decline in ETR max /A gross ). The results imply that elevated CO 2 level could ameliorate some of the detrimental effects of salinity, conferring higher tolerance and survival of S. rebaudiana, a highlydesired feature with the forthcoming era of global changes. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  13. Development and Validation of an Enzymatic Method To Determine Stevioside Content from Stevia rebaudiana.

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    Udompaisarn, Somsiri; Arthan, Dumrongkiet; Somana, Jamorn

    2017-04-19

    An enzymatic method for specific determination of stevioside content was established. Recombinant β-glucosidase BT_3567 (rBT_3567) from Bacteroides thetaiotaomicron HB-13 exhibited selective hydrolysis of stevioside at β-1,2-glycosidic bond to yield rubusoside and glucose. Coupling of this enzyme with glucose oxidase and peroxidase allowed for quantitation of stevioside content in Stevia samples by using a colorimetric-based approach. The series of reactions for stevioside determination can be completed within 1 h at 37 °C. Stevioside determination using the enzymatic assay strongly correlated with results obtained from HPLC quantitation (r 2 = 0.9629, n = 16). The percentages of coefficient variation (CV) of within day (n = 12) and between days (n = 12) assays were lower than 5%, and accuracy ranges were 95-105%. This analysis demonstrates that the enzymatic method developed in this study is specific, easy to perform, accurate, and yields reproducible results.

  14. Nutritional composition of Stevia rebaudiana, a sweet herb, and its hypoglycaemic and hypolipidaemic effect on patients with non-insulin dependent diabetes mellitus.

    Science.gov (United States)

    Ritu, Mathur; Nandini, Johri

    2016-09-01

    The prevalence of diabetes is rapidly rising all over the globe at an alarming rate. India shelters the highest number of diabetics and is thus known as the 'Diabetes Capital of the World'. The chemical management of diabetes has side effects and hence the present study was undertaken to assess the hypoglycaemic and hypolipidaemic effect of Stevia rebaudiana in patients with type 2 diabetes, non-insulin dependent diabetes mellitus (NIDDM). Its nutritional composition and use as a sweetener substitute were also assessed. Chemical analysis of dried Stevia leaf powder revealed it to be a nutritious herb with a good iron and fibre content. Intervention trials in diabetics revealed that it significantly lowered fasting and post-prandial blood glucose levels. The serum triglycerides and VLDL-C levels were also significantly reduced. Hence it can be said that Stevia can safely be used as an anti-diabetic herb, as a sweetener substitute and may help to prevent cardiovascular diseases in patients with long-standing diabetes. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  15. Caracterización genética molecular de materiales procesados de Stevia rebaudiana utilizando la técnica de microsatélites

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    Giovanni Garro-Monge

    2014-09-01

    En este estudio se analizó la amplificación de los ISSR S3, S4, S5, S10, S11, S12 y S15 a partir de muestras de ADN de S. rebaudiana, provenientes de plantas cultivadas en invernadero. Las muestras de ADN se obtuvieron mediante tres diferentes protocolos de extracción a partir de material seco y fresco de la planta. De acuerdo con los resultados obtenidos, los ISSR S3, S4, S5 y S11 representan una buena opción para la detección de polimorfismos en esta especie, y por tanto para la identificación molecular de la especie. Se debe comprobar si los ISSR S10, S12 y S15 son capaces de amplificar segmentos de ADN de Stevia, ya que bajo las condiciones de trabajo de esta investigación no se logró la amplificación de ADN con estos imprimadores. Se considera que los ISSR son marcadores universales y se podría identificar individualmente cada cultivar de Stevia que vaya a destinarse a la producción comercial de esteviósidos, pero es necesario repetir los protocolos descritos con diferentes variedades e incluso se puede trabajar con diferentes especies del género Stevia, para obtener una caracterización general.

  16. ESTUDIO INICIAL DE LA POSIBLE INCORPORACIÓN DE DIFERENTES CONCENTRACIONES DE ESTEVIA EN GOMINOLAS

    OpenAIRE

    MARZAL SARRION, MAR

    2015-01-01

    [EN] In this project the incorporation of different concentrations of Stevia rebaudiana (stevia) in jellies has been evaluated. The initial contamination of the leaves of stevia, a commercial preparation of used stevia as a sweetener, and its antimicrobial capacity versus the growth of Escherichia coli 101 at 20 and 37 °C during 6 days has been studied. The introduction of different concentrations (1 %, 2.5 % and 5 %) of the infusion of stevia in jellies was organoleptically assessed. The res...

  17. Effects of Temperature, Light and Pre-Chilling on Seed Germination of Stevia rebaudiana (Bertoni Bertoni Accessions

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    Mario Macchia

    Full Text Available Stevia rebaudiana (Bertoni Bertoni is a perennial shrub of the Asteraceae family native to Paraguay and Brazil where it has been used for several years as a sweetener. It is a short-day species, with a critical light requirement for flowering of roughly 13 hours. In plants whose biological cycle is strongly photoperiod-dependent, latitude is one of the major factors influencing reproduction. Late flowering may adversely affect seed production if this occurs during a season that is unfavorable to pollination. At Italian latitudes, this species often gives scanty seed production, with low germination rate and poor germination energy of seeds produced. In 2001 four accessions have been grown for seed production in a field plot experiment in Central Italy. The various accessions were found to exhibit noticeably different photoperiod requirements, which affected flowering time (from late August to the end of September and consequently also the conditions of achene filling and ripening. Late flowering and seed ripening occurred during autumn season, unfavourable to complete seed formation, leading to an increase in the empty seed percentage recorded for each accession. Detailed germination trials were therefore undertaken using seeds collected from plants of the different accessions in order to assess the quality of the seeds produced. Various germination methods have been tested in a controlled environment adopting four different temperatures (20° C, 25° C constant temperature and 15/25° C, 20/30° C (16/8h alternating temperature in light or darkness with or without pre-chilling. Germination rates varied over an extensive range (germination percentage from 9 to 83%, mainly due to the divergent specific characteristics of the material examined and the different treatments studied. At all temperatures tested, the most earlier accession, showed the higher germination percentages (54-83% while the latest accession was among those with the lowest

  18. Hydrogenation of the Exocyclic Olefinic Bond at C-16/C-17 Position of ent-Kaurane Diterpene Glycosides of Stevia rebaudiana Using Various Catalysts

    Science.gov (United States)

    Chaturvedula, Venkata Sai Prakash; Prakash, Indra

    2013-01-01

    Catalytic hydrogenation of the exocyclic double bond present between C16 and C17 carbons of the four ent-kaurane diterpene glycosides namely rebaudioside A, rebaudioside B, rebaudioside C, and rebaudioside D isolated from Stevia rebaudiana has been carried out using Pt/C, Pd(OH)2, Rh/C, Raney Ni, PtO2, and 5% Pd/BaCO3 to their corresponding dihydro derivatives with 17α and 17β methyl group isomers. Reactions were performed using the above-mentioned catalysts with the solvents methanol, water, and ethanol/water (8:2) under various conditions. Synthesis of reduced steviol glycosides was performed using straightforward chemistry and their structures were characterized on the basis of 1D and 2D NMR spectral data, including a comparison with reported spectral data. PMID:23896597

  19. Investigation of the photochemical changes of chlorogenic acids induced by ultraviolet light in model systems and in agricultural practice with Stevia rebaudiana cultivation as an example.

    Science.gov (United States)

    Karaköse, Hande; Jaiswal, Rakesh; Deshpande, Sagar; Kuhnert, Nikolai

    2015-04-08

    Mono- and diacyl chlorogenic acids undergo photochemical trans-cis isomerization under ultraviolet (UV) irradiation. The photochemical equilibrium composition was established for eight selected derivatives. In contrast to all other dicaffeoylquinic acid derivatives, cynarin (1,3-dicaffeoylquinic acid) undergoes a [2 + 2] photochemical cycloaddition reaction, constituting a first example of Schmidt's law in a natural product family. The relevance of photochemical isomerization in agricultural practice was investigated using 120 samples of Stevia rebaudiana leave samples grown under defined cultivation conditions. Ratios of cis to trans chlorogenic acids were determined in leaf samples and correlated with climatic and harvesting conditions. The data indicate a clear correlation between the formation of cis-caffeoyl derivatives and sunshine hours prior to harvesting and illustrate the relevance of UV exposure to plant material affecting its phytochemical composition.

  20. Effect of Stevia rebaudiana addition on bioaccessibility of bioactive compounds and antioxidant activity of beverages based on exotic fruits mixed with oat following simulated human digestion.

    Science.gov (United States)

    Carbonell-Capella, Juana M; Buniowska, Magdalena; Esteve, María J; Frígola, Ana

    2015-10-01

    In order to determine the impact of Stevia rebaudiana (SR) addition on bioactive compounds bioaccessibility of a new developed functional beverage based on exotic fruits (mango juice, papaya juice and açaí) mixed with orange juice and oat, an in vitro gastrointestinal digestion was performed. Ascorbic acid, total carotenoids, total phenolics, total anthocyanins, total antioxidant capacity and steviol glycosides were evaluated before and after a simulated gastrointestinal digestion. Salivary and gastric digestion had no substantial effect on any of the major phenolic compounds, ascorbic acid, total antioxidant capacity and steviol glycosides, whereas carotenoids and anthocyanins diminished significantly during the gastric step. All analysed compounds were significantly altered during the pancreatic-bile digestion and this effect was more marked for carotenoids and total anthocyanins. However, phenolic compounds, anthocyanins, total antioxidant capacity and steviol glycosides bioaccessibility increased as did SR concentration. Ascorbic acid bioaccessibility was negatively affected by the SR addition. Copyright © 2015. Published by Elsevier Ltd.

  1. Hydrogenation of the Exocyclic Olefinic Bond at C-16/C-17 Position of ent-Kaurane Diterpene Glycosides of Stevia rebaudiana Using Various Catalysts

    Directory of Open Access Journals (Sweden)

    Indra Prakash

    2013-07-01

    Full Text Available Catalytic hydrogenation of the exocyclic double bond present between C16 and C17 carbons of the four ent-kaurane diterpene glycosides namely rebaudioside A, rebaudioside B, rebaudioside C, and rebaudioside D isolated from Stevia rebaudiana has been carried out using Pt/C, Pd(OH2, Rh/C, Raney Ni, PtO2, and 5% Pd/BaCO3 to their corresponding dihydro derivatives with 17α and 17β methyl group isomers. Reactions were performed using the above-mentioned catalysts with the solvents methanol, water, and ethanol/water (8:2 under various conditions. Synthesis of reduced steviol glycosides was performed using straightforward chemistry and their structures were characterized on the basis of 1D and 2D NMR spectral data, including a comparison with reported spectral data.

  2. SINTOMAS DE DESORDENS NUTRICIONAIS EM ESTÉVIA Stevia rebaudiana (Bert. Bertoni

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    O. F. de Lima Filho

    1997-01-01

    Full Text Available São descritos e discutidos os sintomas de deficiência de N, P, K, Ca, Mg e B, e de toxicidade de B e Zn, em estévia cultivada em solução nutritiva. -N: desenvolvimento reduzido, folhas pequenas, pouca ramificação, e amarelecimento generalizado. -P: crescimento reduzido. -K: folhas encurvadas, precocemente, para baixo, aparentando menor teor de água; amarelecimento e necrose das folhas mais velhas e menor proporção de ramos. -Ca: pontuações escuras, clorose e necrose nas folhas, além de murchamento e colapso da porção superior do caule. -Mg: clorose internerval em algumas folhas mais velhas, em forma de "V" invertido. -B: raízes com diminuição do crescimento, engrossamento, ramificação curta e escurecimento geral; clorose, necrose e deformação das folhas; em folhas jovens, com sintomas, houve menor teor de clorofila; caule com rachaduras de coloração avermelhada, semelhante às nervuras salientes das folhas; menor proporção de ramos. Plantas com toxicidade de B exibiram manchas pardas nas bordas e ápices das folhas mais velhas, seguido de necrose. Plantas com excesso de Zn exibiram manchas necróticas, inicialmente nas folhas mais velhas, de tamanho e forma irregulares, levando ao murchamento e morte da planta.Symptoms of the deficiencies of N, P, K, Ca, Mg and B, as well as the toxicity symptoms of B and Zn in stevia plants, growing in nutrient solution, are described. -N: reduced development, small leaves, few branches and generalized yellowing. -P: reduced growth. -K: leaves curving downward, seeming to have lower water content, yellowing and necrosis of older leaves and smaller stem plus branches proportion. -Ca: dark spots, chlorosis and necrosis in leaves as well as wilting and collapse at the upper portion of stem. -Mg: interveinal paling in some older leaves, with an inverted V appearance. -B: roots with growth reduced, thickening, short ramification and general darkening; chlorosis followed by necrosis and

  3. Comparação de diferentes metodologias para obtenção de cultura de calos de Stevia rebaudiana (Bertoni Bertoni - DOI: 10.4025/actascihealthsci.1090 Comparison of different methodologies for obtaining callus cultures from Stevia rebaudiana (Bertoni Bertoni - DOI: 10.4025/actascihealthsci.1090

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    Regina Aparecida Correia Gonçalves

    2008-03-01

    Full Text Available As folhas de Stevia rebaudiana (Bertoni Bertoni (Asteraceae contêm glicosídeos diterpenóides (GDS, que são cerca de 300 vezes mais doce que a sacarose a 4%. O objetivo deste estudo foi avaliar a formação de calos, a partir de folhas obtidas in vivo e in vitro de S. rebaudiana em dois meios já descritos na literatura: Murashige e Skoog (MS, suplementado com 3 mg L-1 de ácido 2,4-diclorofenóxiacético (2,4-D, e o MS suplementado com 1 mg L-1 de ácido naftalenoacético (ANA e 0,5 mg L-1 de 6-benzilaminopurina 6-BAP e um desenvolvido em nosso laboratório o Woody Plant Medium (WPM, suplementado com 6 mg L-1 de ANA e 4 mg L-1 de cinetina (CIN. Os explantes obtidos in vitro iniciaram a formação de calos um pouco mais rapidamente que os das folhas de plantas advindas da natureza. A utilização dos nutrientes do meio WPM, associada a uma combinação de fitorreguladores adequada, proporcionou velocidade de indução e multiplicação de calos bem maiores que as apresentadas nos meios que empregaram os nutrientes do MS. Novos experimentos serão realizados, depois de alcançada a estabilidade genética dos calos, visando avaliar a capacidade destes em biossintetizar os GDSThe leaves from Stevia rebaudiana (Bertoni Bertoni (Asteraceae contain diterpenoid glycosides (GDS, which are almost 300 times sweeter than sucrose at 4%. The subject of this study was to evaluate the callus-formation from in vivo and in vitro leaves of Stevia rebaudiana in two already described in literature: Murashige and Skoog (MS supplemented with 3 mg L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D; MS supplemented with 1 mg L-1 of naphthaleneacetic acid (NAA and 0.5 mg L-1 of 6-benzylaminopurine (6-BAP and other developed in our laboratory the Woody Plant Medium (WPM with 6 mg L-1 of NAA and 4 mg L-1 of Kinetin (KIN. The explants obtained in vitro initiated callus formation faster than leaves from natural plants. The utilization of WPM nutrients, associated with an

  4. Development of an efficient high-performance thin layer chromatography method for determination of jasmonic acid in leaf tissue of Stevia rebaudiana (Bertoni) Bertoni.

    Science.gov (United States)

    Kilam, Divya; Saifi, M; Agnihotri, A; Abdin, M Z

    2017-07-01

    Determination of endogenous levels of jasmonic acid (JA) is essential, as it plays a pivotal role in the physiological processes during a plant's life cycle. A high performance thin layer chromatography (HPTLC) method was developed for the detection and quantification of JA in leaf extracts of medicinal plant, Stevia rebaudiana (Bertoni) Bertoni. The separation was achieved using the solvents ethyl acetate-benzene (1:1, v/v) as the mobile phase, followed by scanning and quantification at 295 nm. Densitometric analysis of leaf extract resulted in compact spots for JA at Rf = 0.45 ± 0.02. The linear regression analysis showed good relationship with r value. The recovery rate of JA indicated good reproducibility and repeatability of the assay. The statistical analysis proved the reproducibility of the method; therefore, it can be employed for routine quantification of JA in different tissue samples of S. rebaudiana and may also be extrapolated to other biological samples.

  5. Effect of zinc oxide (ZnO) nanoparticles on physiology and steviol glycosides production in micropropagated shoots of Stevia rebaudiana Bertoni.

    Science.gov (United States)

    Javed, Rabia; Usman, Muhammad; Yücesan, Buhara; Zia, Muhammad; Gürel, Ekrem

    2017-01-01

    This study aims to address the effects of different concentrations (0, 0.1, 1.0, 10, 100 or 1000 mg L -1 ) of engineered zinc oxide (ZnO) nanoparticles (34 nm in size) on growth parameters, steviol glycosides (rebaudioside A and stevioside) production and antioxidant activities in the tissue culture grown shoots of Stevia rebaudiana Bertoni. The highest percentage of shoot formation (89.6%) at 1 mg L -1 of ZnO nanoparticles concentration suggests a positive influence of ZnO nanoparticles on S. rebaudiana growth as compared to other treatments with or without ZnO nanoparticles. Additionally, HPLC results illustrate a significant enhancement of steviol glycosides (almost doubled as compared to the control) in micropropagated shoots grown under an oxidative stress of 1 mg L -1 of ZnO nanoparticles. This finding is further affirmed by an increased 2,2-diphenyl-1-picryl hydrazyl (DPPH) scavenging activity, total anti-oxidant capacity, total reducing power, total flavonoid content and total phenolic content, with an ascending oxidative pressure and generation of reactive oxygen species (ROS). However, the antioxidant activities, formation of secondary metabolites and the physiological parameters showed a sudden decline after crossing a threshold of 1 mg L -1 concentration of ZnO nanoparticles and falls to a minimum at 1000 mg L -1 , elucidating maximum phytotoxic effect of ZnO nanoparticles at this concentration. This is the first study evaluating both the favorable and adverse effects of ZnO nanoparticles employed to a highly valuable medicinal plant, S. rebaudiana. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  6. Effects of methyl jasmonate, on stevioside and rebaudioside A content and expression of the ent-Kaurenoic acid 13-hydroxylase gene in Stevia rebaudiana Bert. in vitro

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    Mehrdad Behmanesh

    2014-08-01

    Full Text Available Glycosides are a form of secondary metabolites that consist variety compounds and in some cases can play a role in primary metabolism. Steviol is lipophilic skeleton of Stevioside and Rebaudioside A, two main glycosides of Stevia rebuadiana. Steviol glycosides which are synthesized in S.rebaudiana have important medical and nutritional values as high intensity natural sweeteners. Steviol is synthesized from Kaurenoic acid in chloroplastic Terpenoid pathway that mediated by Kaurenoic acid 13-hydroxylase. In this study, HPLC method and RT-PCR were performed for quantification of glycosides and gene expression (ent-Kaurenoic acid 13-hydroxylase respectively. Methyl jasmonate treatment (at 20 micromolar in vitro induced glycoside biosynthesis significantly (P≤0.05 whereas higher concentration of Methyl jasmonate (100 µM caused a decrease in glycoside production and growth. The most glycoside content of the plant was three days after treatment. Also Methyl jasmonate treatment caused an increase in ent-Kaurenoic 13-hydroxylase gene expression from 6 hours to 48 hours (after treatment Results showed that biosynthesis of Stevia glycosides was probably a defense mechanism against pathogens and herbivore insects. Also we found that different concentrations of Methyl jasmonate, alter the ratio between glycosides rather than the increase in glycoside contents.

  7. Effect of salts (NaCl and Na2CO3) on callus and suspension culture of Stevia rebaudiana for Steviol glycoside production.

    Science.gov (United States)

    Gupta, Pratibha; Sharma, Satyawati; Saxena, Sanjay

    2014-03-01

    Steviol glycosides are natural non-caloric sweeteners which are extracted from the leaves of Stevia rebaudiana plant. Present study deals the effect of salts (NaCl and Na2CO3) on callus and suspension culture of Stevia plant for steviol glycoside (SGs) production. Yellow-green and compact calli obtained from in vitro raised Stevia leaves sub-cultured on MS medium supplemented with 2.0 mg l(-1) NAA and different concentrations of NaCl (0.05-0.20%) and Na2CO3 (0.0125-0.10%) for 2 weeks, and incubated at 24 ± 1 °C and 22.4 μmol m(-2) s(-1) light intensity provided by white fluorescent tubes for 16 h. Callus and suspension biomass cultured on salts showed less growth as well as browning of medium when compared with control. Quantification of SGs content in callus culture (collected on 15th day) and suspension cultures (collected at 10th and 15th days) treated with and without salts were analyzed by HPLC. It was found that abiotic stress induced by the salts increased the concentration of SGs significantly. In callus, the quantity of SGs got increased from 0.27 (control) to 1.43 and 1.57% with 0.10% NaCl, and 0.025% Na2CO3, respectively. However, in case of suspension culture, the same concentrations of NaCl and Na2CO3 enhanced the SGs content from 1.36 (control) to 2.61 and 5.14%, respectively, on the 10th day.

  8. Evaluation of supplementary stevia (Stevia rebaudiana, bertoni) leaves and stevioside in broiler diets: effects on feed intake, nutrient metabolism, blood parameters and growth performance.

    Science.gov (United States)

    Atteh, J O; Onagbesan, O M; Tona, K; Decuypere, E; Geuns, J M C; Buyse, J

    2008-12-01

    A perennial schrub, stevia, and its extracts are used as a natural sweetener and have been shown to possess antimicrobial properties. Stevia contains high levels of sweetening glycosides including stevioside which is thought to possess antimicrobial and antifungal properties. Little is known about the nutritional value of the schrub in livestock. This study determined the potential use of the shrub as a prebiotic animal feed supplement in light of the recent ban on the use of antibiotics in animal feed and the role of its constituent stevioside in the effects of the shrub. Male Cobb broiler chicks were fed a basal broiler diet without antibiotic but with performance enhancing enzyme mix (positive control), a basal diet without antibiotic and enzymes (negative control), or diets in which 2% of the negative control diet was replaced with either dried ground stevia leaves or 130 ppm pure stevioside during 2 week starter and 2 week grower periods. Body weight gains, feed conversion, abdominal fat deposition, plasma hormone and metabolites and caecal short chain fatty acids (SCFA) were measured in the broilers at 2 and 4 weeks of age. There was no significant effect of the treatments on feed intake during the starter period but birds fed diet supplemented with stevia leaves and stevioside consumed more feed (p growth promoters are beneficial to the broilers only during the starter stage and that inclusion of stevia leaves or stevioside has no beneficial effect on the performance of broilers.

  9. Effectiveness of Stevia Rebaudiana Whole Leaf Extract Against the Various Morphological Forms of Borrelia Burgdorferi in Vitro

    Science.gov (United States)

    Theophilus, P. A. S.; Victoria, M. J.; Socarras, K. M.; Filush, K. R.; Gupta, K.; Luecke, D. F.; Sapi, E.

    2015-01-01

    Lyme disease is a tick-borne multisystemic disease caused by Borrelia burgdorferi. Administering antibiotics is the primary treatment for this disease; however, relapse often occurs when antibiotic treatment is discontinued. The reason for relapse remains unknown, but recent studies suggested the possibilities of the presence of antibiotic resistant Borrelia persister cells and biofilms. In this study, we evaluated the effectiveness of whole leaf Stevia extract against B. burgdorferi spirochetes, persisters, and biofilm forms in vitro. The susceptibility of the different forms was evaluated by various quantitative techniques in addition to different microscopy methods. The effectiveness of Stevia was compared to doxycycline, cefoperazone, daptomycin, and their combinations. Our results demonstrated that Stevia had significant effect in eliminating B. burgdorferi spirochetes and persisters. Subculture experiments with Stevia and antibiotics treated cells were established for 7 and 14 days yielding, no and 10% viable cells, respectively compared to the above-mentioned antibiotics and antibiotic combination. When Stevia and the three antibiotics were tested against attached biofilms, Stevia significantly reduced B. burgdorferi forms. Results from this study suggest that a natural product such as Stevia leaf extract could be considered as an effective agent against B. burgdorferi. PMID:26716015

  10. The effect of Stevia rebaudiana on serum omentin and visfatin level in STZ-induced diabetic rats.

    Science.gov (United States)

    Akbarzadeh, Samad; Eskandari, Fatemeh; Tangestani, Hadis; Bagherinejad, Somaieh Tangerami; Bargahi, Afshar; Bazzi, Parviz; Daneshi, Adel; Sahrapoor, Azam; O'Connor, William J; Rahbar, Ali Reza

    2015-03-01

    Recently the role of adipocytokines in relationship to incidence of diabetes has been demonstrated. One of the medicinal plants that are used in the treatment of diabetes is stevia. This study investigates the effect of stevia on serum omentin and visfatin levels as novel adipocytokines in diabetic induced rats to find potential mechanisms for the anti hyperglycemic effect of stevia. Forty male wistar rats weighing 180-250 g were induced with diabetes by intraperitoneal injection of streptozotocin (STZ). The animals were divided into 5 groups of 8. Rats in group 1 (non-diabetic control) and group 2 (diabetic control) were treated with distilled water, and the rats in the treated groups, group 3 (T250), group 4 (T500), and group 5 (T750) were treated with stevia, gavaged every day at 9 a.m. in doses of 250, 500, and 750 mg/kg, respectively. At the end of the study significant reductions in fasting blood sugar (FBS), the homeostasis model assessment insulin resistance (HOMA-IR), triglyceride (TG), alkaline phosphatase (ALP), and Omentin level were found in groups 3 and 4 in comparison with group 2. Pancreatic histopathology slides demonstrated that stevia extract did not induce any increase in the number of β-cells. The conclusion is that prescription of stevia in the doses of 250 and 500 mg/kg/d decreases the omentin level indirectly via activating insulin sensitivity and lowering blood glucose in STZ-induced diabetic rats.

  11. The Influence of the Rebaudioside A Content of Stevia (Stevia rebaudiana Bertoni) on the Determination of Sweetness Equivalence in Bittersweet Chocolates, Using the Time-Intensity Analysis.

    Science.gov (United States)

    Azevedo, Bruna M; Ferreira, Janaína M M; Luccas, Valdecir; Bolini, Helena M A

    2016-12-01

    The consumption of diet products has increased greatly in recent years. The objectives of the study were to develop a bittersweet chocolate added inulin and stevias with different rebaudioside A contents (60%, 80%, and 97%). Five chocolate samples were formulated with different sucrose concentrations to determine the ideal sucrose concentration for bittersweet chocolate. The use of just-about-right scale identified an ideal sucrose concentration of 47.5% (w/w). The sweetness equivalence in sugar-free bittersweet chocolates was determined by the time-intensity method by 14 selected and trained judges. The data collected during each session of sensory evaluation furnished the following parameters in relation to the sweet stimulus: Imax (maximum intensity recorded), Timax (time at which the maximum intensity was recorded), Area (area of time × intensity curve), and Ttot (total duration time of the stimulus). The time-intensity analysis indicated that the percentages of rebaudioside A did not interfere with the sweetness intensity of the sweetener stevia in bittersweet chocolate and there was no significant difference in the concentrations tested (0.16%, 0.22%, 0.27%) of each stevia, in relation to the parameters evaluated. In addition, the reduction in fat content did not alter the perception of the sweetness intensity of the samples. These results showed important information to research and development of chocolate products. Therefore, the use of the lowest stevia concentration tested (0.16%) is the most indicated for use, since this quantity was sufficient to reach the ideal sweetness of the product, so there was no point in adding more. © 2016 Institute of Food Technologists®.

  12. La Stevia rebaudiana como coadyuvante en la prevención y el control de la caries dental: una revisión de literatura

    Directory of Open Access Journals (Sweden)

    Andrea Elena Paredes Vélez

    2016-07-01

    Full Text Available Introducción: La caries dental es la enfermedad crónica más prevalente en el mundo, y en Colombia, como en otros países, es considerada como un problema de salud pública. Es una enfermedad compleja, dinámica, y para su desarrollo intervienen muchos factores; la dieta rica en carbohidratos, es uno de los más significativos. La sacarosa se ha relacionado con problemas de salud como la caries, por ello, es deseable su reemplazo por edulcorantes con menos efectos adversos, y que aporten beneficios a la salud general y bucal de los humanos. Objetivo: fundamentar, si la Stevia, podría ser considerada como un coadyuvante en la prevención y control de la caries dental. Metodología: se realizó una búsqueda de literatura utilizando las bases de datos: Pubmed, Academic Search Complete, Embase, ScienceDirect, y el motor de búsqueda Google académico, mediante los términos MESH: “Dental Caries”, “Stevia”, “Anti-bacterial Agents”, “Sweetening Agents” y palabras clave en español: “Caries dental”, “Edulcorantes”, “Stevia”. Resultados: Los estudios revisados in vivo e in vitro mostraron: actividad antibacteriana de extractos de Stevia sobre microorganismos relacionados con caries dental, bajo potencial acidogénico y disminución en la formación de biopelícula dental, debido a la disminución de la hidrofobicidad celular e inhibición de la síntesis de polisacáridos extracelulares. Conclusiones: Aunque la evidencia científica aún es insuficiente, la Stevia rebaudiana es una adecuada candidata a reemplazar la sacarosa y puede considerarse como coadyuvante potencial para disminuir los niveles de caries dental, sin embargo, se recomienda realizar más estudios controlados y aleatorizados para que dicho rol se confirme.

  13. Antioxidant activities of aqueous extract from Stevia rebaudiana stem waste to inhibit fish oil oxidation and identification of its phenolic compounds.

    Science.gov (United States)

    Yu, Hui; Yang, Gangqiang; Sato, Minoru; Yamaguchi, Toshiyasu; Nakano, Toshiki; Xi, Yinci

    2017-10-01

    We investigated the potential for exploiting Stevia rebaudiana stem (SRS) waste as a source of edible plant-based antioxidants finding for the first time that the hot water extract of SRS had significantly higher antioxidant activity against fish oil oxidation than that of the leaf, despite SRS extract having lower total phenolic content, DPPH radical scavenging activity and ORAC values. To locate the major antioxidant ingredients, SRS extract was fractionated using liquid chromatography. Five phenolic compounds (primary antioxidant components in activity-containing fractions) were identified by NMR and HR-ESI-MS: vanillic acid 4-O-β-d-glucopyranoside (1), protocatechuic acid (2), caffeic acid (3), chlorogenic acid (4) and cryptochlorogenic acid (5). Further analysis showed that, among compounds 2-5, protocatechuic acid had the highest capacity to inhibit peroxides formation, but exhibited the lowest antioxidant activities in DPPH and ORAC assays. These results indicate that SRS waste can be used as strong natural antioxidant materials in the food industry. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Agrobacterium tumefaciens-mediated transgenic plant and somaclone production through direct and indirect regeneration from leaves in Stevia rebaudiana with their glycoside profile.

    Science.gov (United States)

    Khan, Shamshad Ahmad; Ur Rahman, Laiq; Shanker, Karuna; Singh, Manju

    2014-05-01

    Agrobacterium tumefaciens (EHA-105 harboring pCAMBIA 1304)-mediated transgenic plant production via direct regeneration from leaf and elite somaclones generation through indirect regeneration in Stevia rebaudiana is reported. Optimum direct regeneration frequency along with highest transformation frequency was found on MS + 1 mg/l BAP + 1 mg/l NAA, while indirect regeneration from callus was obtained on MS + 1 mg/l BAP + 2 mg/l NAA. Successful transfer of GUS-positive (GUS assay and PCR-based confirmation) transgenic as well as four somaclones up to glasshouse acclimatization has been achieved. Inter-simple sequence repeat (ISSR) profiling of transgenic and somaclonal plants showed a total of 113 bands, out of which 49 were monomorphic (43.36 %) and 64 were polymorphic (56.64 %). Transgenic plant was found to be closer to mother plant, while on the basis of steviol, stevioside, and rebaudioside A profile, somaclone S2 was found to be the best and showed maximum variability in ISSR profiling.

  15. Effect of Nitrogen Level and Natural Zeolite on Qualitative and Quantitative Function of Stevia rebaudiana (Bertoni in Ahvaz Climatic Condition

    Directory of Open Access Journals (Sweden)

    A Bakgshandeh

    2016-10-01

    Full Text Available Introduction Nowadays due to population growth and higher levels of welfare, one of the main social problems is providing appropriate food resources. In this regard, finding the alternative food resources, improvement of existing technologies and providing new technologies seem inevitable. Although sugar is a very important substance and has special place in household’s basket and economy, the harmful effects of excessive consumption of sugar should not be neglected. Stevia is a plant with a widespread root system which has a couple of years lifetime. The stems of Stevia are brittle and small and produce elliptical leaves. At first the leaves will be dried and then they will be powdered or wetted in the water and will be used for sweetening of beverages, sweet drinks, chewing gums, chocolates, cakes, etc. Stevia is one of 950 genera belonging to the family of Asteraceae plant. Stevia leaves are used for sweetening foods. Sweetness of Stevia comes from the d. terpenoides glycosides that exist in different parts of the plant. Glycosides are the result of secondary metabolism in the plants. In different components of the Stevia body, there are about 10 major glycoside compositions which the steviozede and Rbadiozide glycosides have more importance in sweetening property of Stevia compared to other sweet glycosides. Nitrogen is considered as one of the key elements in the nutrition of crops and the most important element in limiting the plant growth. Due to increased cation exchange capacity and a high tendency to absorb and retain ammonium, use of clinoptilolite on agricultural lands can have an effective role in reduction of nutrient washing especially nitrogen from soil. Materials and Methods In order to investigate the effect of different levels of nitrogen and natural zeolite on qualitative and quantitative yield of Stevia in Ahvaz climate, a trial agricultural research field was selected at the farm of agricultural and natural resources of

  16. Influence of NaCl on Biochemical Parameters of Two Cultivars of Stevia rebaudiana Regenerated in vitro

    Directory of Open Access Journals (Sweden)

    Sharuti Rathore

    2014-05-01

    Full Text Available Soil salinity occupies a prominent place among the soil problems that threaten the sustainability of agriculture over a vast area in the world. It affects plant morpho-physiology and ultimately leads to reduction in productivity. It is essential to test important medicinal plants for their salinity tolerance as research efforts aim to explore economic benefits under saline conditions. Keeping in view the importance of Stevia and salinity, present study had been designed to investigate the effect of salinity on biochemical parameters in two Stevia genotypes. Two node microcuttings were subjected to MS media supplemented with different NaCl concentrations (0, 25, 50, 75, 100, 125 mM. Chlorophyll amount was observed to be decreased as compared to sugars, proline and phenols with increased salt concentrations.

  17. Effects of Stevia rebaudiana (Bertoni) extract and N-nitro-L-arginine on renal function and ultrastructure of kidney cells in experimental type 2 Diabetes.

    Science.gov (United States)

    Ozbayer, Cansu; Kurt, Hulyam; Kalender, Suna; Ozden, Hilmi; Gunes, Hasan V; Basaran, Ayse; Cakmak, Ecir A; Civi, Kismet; Kalender, Yusuf; Degirmenci, Irfan

    2011-10-01

    Diabetes is the leading cause of chronic renal failure. Our purpose was to determine the effects of N-nitro-l-arginine (l-NNA) and an extract of Stevia rebaudiana (Bertoni) (SrB) leaves on renal function in streptozotocin-nicotinamide (STZ-NA)-induced diabetic rats. Rats were divided into seven groups. Three of these groups were controls. Diabetes was induced by STZ-NA in the other four. Diabetic rats were treated with SrB (200 mg/kg), L-NNA (100 mg/kg), or SrB + L-NNA for 15 days after 5-8 weeks of diabetes. At the end of the experiments, urine and blood samples were collected from the rats, and kidney tissue samples were collected with the animals under ether anesthesia. Renal filtration changes were determined by measuring urine pH, urine volume, and serum and urine creatinine. Nitric oxide synthase (NOS) activity was measured in kidney homogenates. Alterations in kidney ultrastructure were determined by electron microscopy, and histological changes were examined by hematoxylin and eosin staining. No statistical differences were observed in urine creatinine or creatinine clearance. Even so, we observed higher NOS activity in SrB-treated diabetic rats. SrB-treated diabetic rats had less mitochondrial swelling and vacuolization in thin kidney sections than other diabetic groups. The control groups showed normal histological structure, whereas in the diabetic groups, membrane thickening, tubular epithelial cells, and cellular degeneration were observed. Thus, SrB has beneficial effects on diabetes compared with l-NNA. Our results support the validity of SrB for the management of diabetes as well as diabetes-induced renal disorders.

  18. In silico analysis and expression profiling of miRNAs targeting genes of steviol glycosides biosynthetic pathway and their relationship with steviol glycosides content in different tissues of Stevia rebaudiana.

    Science.gov (United States)

    Saifi, Monica; Nasrullah, Nazima; Ahmad, Malik Mobeen; Ali, Athar; Khan, Jawaid A; Abdin, M Z

    2015-09-01

    miRNAs are emerging as potential regulators of the gene expression. Their proven promising role in regulating biosynthetic pathways related gene networks may hold the key to understand the genetic regulation of these pathways which may assist in selection and manipulation to get high performing plant genotypes with better secondary metabolites yields and increased biomass. miRNAs associated with genes of steviol glycosides biosynthetic pathway, however, have not been identified so far. In this study miRNAs targeting genes of steviol glycosides biosynthetic pathway were identified for the first time whose precursors were potentially generated from ESTs and nucleotide sequences of Stevia rebaudiana. Thereafter, stem-loop coupled real time PCR based expressions of these miRNAs in different tissues of Stevia rebaudiana were investigated and their relationship pattern was analysed with the expression levels of their target mRNAs as well as steviol glycoside contents. All the miRNAs investigated showed differential expressions in all the three tissues studied, viz. leaves, flowers and stems. Out of the eleven miRNAs validated, the expression levels of nine miRNAs (miR319a, miR319b, miR319c, miR319d, miR319e, miR319f, miR319h, miRstv_7, miRstv_9) were found to be inversely related, while expression levels of the two, i.e. miR319g and miRstv_11 on the contrary, showed direct relation with the expression levels of their target mRNAs and steviol glycoside contents in the leaves, flowers and stems. This study provides a platform for better understanding of the steviol glycosides biosynthetic pathway and these miRNAs can further be employed to manipulate the biosynthesis of these metabolites to enhance their contents and yield in S. rebaudiana. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  19. Evaluación de métodos de propagación, fertilización nitrogenada y fenología de estevia en condiciones del Valle del Cauca Evaluation of propagation methods, nitrogen fertilization and phenology in stevia in the Cauca Valley, Colombia

    Directory of Open Access Journals (Sweden)

    Carmen R Bonilla C

    Full Text Available En el Centro Experimental de la Universidad Nacional de Colombia sede Palmira se evaluó el prendimiento de estacas apicales y basales de Stevia rebaudiana en tres sustratos de enraizamiento (carbonilla-arena, carbonilla-compost y arena-compost y tres fuentes de nitrógeno (urea 46%N, compost 1.5%N y gallinaza 1% N. El mejor método de propagación fue la estaca apical en sustrato carbonilla-arena 1:1, volumen; las fuentes de nitrógeno no presentaron diferencias significativas en la acumulación de materia seca de hojas. Se diferenciaron los siguientes periodos fenológicos: Emergencia: 6 días después de la siembra (dís, Estado vegetativo: 17 dias, Floración: 71 dís, Fructificación: 115 dís, Senescencia: 169 dís y Rebrote: 201 dísThis research was carried out at the Experimental Center of the National University of Colombia ( Palmira in order to know more about the cropping system of Stevia rebaudiana Bertoni. The research consisted of an evaluation of rooting process and growth of cuttings from basal and apical position of the stem. The results were obtained from three root sustrates (coal ship-sand, coal ship-compost and sand-compost and three different nitrogen sources (urea 46%N, compost 1.5% N and chicken manure 1% N. The results showed that the most productive sustrate was coal ship-sand 1:1 volume. The application of the nitrogen sources had no significant differences in accumulation of leaf dry matter. Phenological observation were as follows: emergence: 6 days after sowing (das, vegetative state: 17 das, flowering: 71 das, fructificatión: 115 das, senility: 169 das and shoot: 201 dís.

  20. Comparison of some antioxidant properties of plant extracts from Origanum vulgare, Salvia officinalis, Eleutherococcus senticosus and Stevia rebaudiana.

    Science.gov (United States)

    Vaško, Ladislav; Vašková, Janka; Fejerčáková, Andrea; Mojžišová, Gabriela; Poráčová, Janka

    2014-08-01

    Phenolic compounds from plants are known for their antioxidant properties and have been proposed as therapeutic agents to counteract oxidative stress. However, under normal circumstances, the body only receives a very small amount of these substances in the diet. We have investigated the effect of extracts from known and frequently used plants as part of diet, food seasoning, medicinal tea, and sweetener at different concentrations on the ability to scavenge free radicals, to affect antioxidant enzymes, and finally in the survival of cancer cell lines. We found extract concentrations of about 100 μg.ml(-1) more indicative in the assessment of all parameters investigated. Ginseng possessed a very good ability to scavenge superoxide and hydroxyl radicals, while stevia also manifested significant effects against hydroxyl radicals. Both extracts also showed NO decomposition ability. The antioxidant defense system against the excessive production of radicals in mitochondria was sufficient. In contrast, the range of operating concentrations for sage and oregano mainly presented no significant effects against reactive oxygen and nitrogen species. Taken together with the significantly reduced activity of glutathione peroxidase, this led to the depletion of glutathione. The demonstrated modulation of redox state capability was sufficient to affect the viability of all tested cancer cell lines, but especially A-549, CEM and HeLa by oregano extract. Results support the promising role of the tested extracts as a source of compounds for further in vivo studies with the ability to powerfully interfere with or modify the redox state of cells according to the type of disease, which is expected to be associated with oxidative stress.

  1. Stevia: Medicinal Miracles and Therapeutic Magic

    OpenAIRE

    Sharangi, Amit Baran; Bhutia, Pemba Hissay

    2016-01-01

    Stevia (Stevia rebaudiana Bert., Asteraceae) has drawn the attention of health conscious fitness lovers all over the planet as a non-caloric sweetener. It was regarded by almost the whole world replacing saccharin. The major sweet compounds that are isolated from the stevia leaves are several glycoside compounds including stevioside, steviolbioside, rebaudiosides and dulcoside. Studies found that kaempferol can reduce risk of pancreatic cancer by 23%.  Taking stevioside as a supplement can re...

  2. Effect of harvest timing on leaf production and yield of diterpene glycosides in Stevia rebaudiana Bert: a specialty perennial crop for Mississippi

    Science.gov (United States)

    Stevia rebaundiana (Bertoni), a perennial shrub of the Asteraceae, is one of the most important sources of non-caloric natural sweeteners. Stevia’s plant extracts and glycosides have been used for several years in Paraguay and Brazil. Several studies suggest that Stevia and its glycosides exert ben...

  3. Base substitution mutations in uridinediphosphate-dependent glycosyltransferase 76G1 gene of Stevia rebaudiana causes the low levels of rebaudioside A: mutations in UGT76G1, a key gene of steviol glycosides synthesis.

    Science.gov (United States)

    Yang, Yong-Heng; Huang, Su-Zhen; Han, Yu-Lin; Yuan, Hai-Yan; Gu, Chun-Sun; Zhao, Yan-Hai

    2014-07-01

    Steviol glycosides, extracted from the leaves of Stevia rebaudiana (Bert) Bertoni, are calorie-free sugar substitute of natural origin with intensely sweet (Boileau et al., 2012). Stevioside and rebaudioside A are the two main kinds of the diterpenic glycosides. We analyzed the concentration of stevioside and rebaudioside A in Stevia leaves of about 500 samples (hybrid progenies) and discovered a mutation plant "Z05" with very low levels of rebaudioside A. Because UGT76G1, a uridinediphosphate-dependent glycosyltransferases, is responsible for the conversion from stevioside to rebaudioside A (Richman et al., 2005), so mutation identification was done by sequencing the candidate gene, UGT76G1. In this study molecular analysis of two strains revealed a heterozygotic nonsense mutation of c.389T > G (p.L121X) in UGT76G1. Meanwhile, we found some amino acid substitutions significant change the protein structure. And the difference of enzyme activity between two strains proved the lack of functionality of UGT76G1 of the mutation "Z05". So the nonsense mutation and amino acid substitution mutation resulted in the low levels of rebaudioside A. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  4. An in vitro and in vivo comparison of the effect of Stevia rebaudiana extracts on different caries-related variables: a randomized controlled trial pilot study.

    Science.gov (United States)

    Brambilla, E; Cagetti, M G; Ionescu, A; Campus, G; Lingström, P

    2014-01-01

    The effect of Stevia extracts on in vitro Streptococcus mutans biofilm formation and in vivo plaque pH was evaluated in this paper. Three 10% solutions containing stevioside, rebaudioside A or sucrose were prepared. MTT assay was used to evaluate microbiological counts in vitro. Twenty volunteers rinsed for 1 min with each solutions, and plaque pH was measured at 7 time points after each rinse. Higher in vitro S. mutans biofilm formation was observed in sucrose solution (p Stevia extracts (F = 99.45, p Stevia extracts can be considered nonacidogenic.

  5. Evaluation of propagation methods, nitrogen fertilization and phenology in stevia in the Cauca Valley, Colombia Evaluación de métodos de propagación, fertilización nitrogenada y fenología de estevia en condiciones del Valle del Cauca

    Directory of Open Access Journals (Sweden)

    Bonilla Correa Carmen Rosa

    2007-09-01

    Full Text Available This research was carried out at the Experimental Center of the National University of Colombia (Palmira in order to know more about the cropping system of Stevia rebaudiana Bertoni. The research consisted of an evaluation of rooting process and growth of cuttings from basal and apical position of the stem. The results were obtained from three root sustrates (coal ship-sand, coal ship-compost and sand-compost and three different nitrogen sources (urea 46%N, compost 1.5% N and chicken manure 1% N. The results showed that the most productive sustrate was coal ship-sand 1:1 volume. The application of the nitrogen sources had no significant differences in accumulation of leaf dry matter. Phenological observation were as follows: emergence: 6 days after sowing (das, vegetative state: 17 das, flowering: 71 das, fructificatión: 115 das, senility: 169 das and shoot: 201 dísEn el Centro Experimental de la Universidad Nacional de Colombia sede Palmira se evaluó el prendimiento de estacas apicales y basales de Stevia rebaudiana en tres sustratos de enraizamiento (carbonilla-arena, carbonilla-compost y arena-compost y tres fuentes de nitrógeno (urea 46%N, compost 1.5%N y gallinaza 1% N. El mejor método de propagación fue la estaca apical en sustrato carbonilla-arena 1:1, volumen; las fuentes de nitrógeno no presentaron diferencias significativas en la acumulación de materia seca de hojas. Se diferenciaron los siguientes periodos fenológicos: Emergencia: 6 días después de la siembra (dís, Estado vegetativo: 17 dias, Floración: 71 dís, Fructificación: 115 dís, Senescencia: 169 dís y Rebrote: 201 dís

  6. Aproximación a la comprensión de un endulzante natural alternativo, la Stevia Rebaudiana Bertoni: producción, consumo y demanda potencial

    OpenAIRE

    Andrés González-Moralejo, Silvia

    2011-01-01

    Licencia Creative Commons Atribución-NoComercial-CompartirIgual 3.0 Venezuela Este artículo se refiere a un producto, la Stevia, capaz de revolucionar el mercado de los endulzantes, debido a sus propiedades totalmente naturales que lo convierten en perfecto sustituto del azúcar y los edulcorantes artificiales. La Stevia se analiza desde un enfoque cualitativo-cuantitativo. Cualitativamente, se pretende mitigar el desconocimiento sobre la misma, ofreciendo un estudio descriptivo de sus prop...

  7. Proyecto de inversión para la elaboración de un edulcorante natural hecho a base de stevia en la provincia de El Oro

    OpenAIRE

    Terán Elizalde, Eric Xavier

    2010-01-01

    Stevia is plant, native to subtropical and tropical South America and Central America. The species Stevia rebaudiana Bertoni, commonly known as sweetleaf, or simply stevia, is widely grown for its sweet leaves. As a sugar substitute,to 300 times the sweetness of sugar, stevia has garnered attention with the rise in demand for lowcarbohydrate,. Medical research has also shown possible benefits of stevia in treating obesity and high blood pressure. Because stevia has a negligible ef...

  8. Microbial production of next-generation stevia sweeteners

    DEFF Research Database (Denmark)

    Olsson, Kim; Carlsen, Simon; Semmler, Angelika

    2016-01-01

    BACKGROUND: The glucosyltransferase UGT76G1 from Stevia rebaudiana is a chameleon enzyme in the targeted biosynthesis of the next-generation premium stevia sweeteners, rebaudioside D (Reb D) and rebaudioside M (Reb M). These steviol glucosides carry five and six glucose units, respectively...

  9. Stevia rebaudiana Bertoni as a source of bioactive compounds: the effect of harvest time, experimental site and crop age on steviol glycoside content and antioxidant properties.

    Science.gov (United States)

    Tavarini, Silvia; Angelini, Luciana G

    2013-07-01

    This study was aimed at identifying the effect of harvest time, experimental site and crop age on the no-calorie sweetener steviol glycosides (SG) and on the antioxidant properties of stevia leaf extracts. The experiment was conducted over two growing seasons at two sites in the northeastern plain of Italy. The results showed that all analysed factors played an important role in defining the SG profile and the antioxidant properties of stevia extracts. A high level of phenols (78.24 mg GAE g⁻¹ DW by Folin-Ciocalteu method) and high antioxidant activity (812.6 µmol Fe²⁺ g⁻¹ DW by FRAP assay) were observed. The inhibition of DPPH free radicals was evaluated and an IC₅₀ mean value of 250 µg mL⁻¹ was obtained. Significant relationships among the total antioxidant capacity and the analysed compounds were found. The results showed the possibility of obtaining, in the tested environments, very high SG yields thanks to the long-day conditions during the spring/summer season. The harvest time played a key role in determining the stevia quality, influencing the rebaudioside A/stevioside ratio. The strong antioxidant properties make very interesting the possibility of using stevia extracts to improve functional food properties. © 2012 Society of Chemical Industry.

  10. KAJIAN: KHASIAT DAN KEAMANAN STEVIA SEBAGAI PEMANIS PENGGANTI GULA

    OpenAIRE

    Mariana Raini; Ani Isnawati

    2012-01-01

    The increasing of diabetic prevalence encouraged scientists to discover sweetener  as sugar substitution. Stevia is a perenial shrub (known as Stevia rebaudiana) of the aster family that has been used for hundred of years to sweetening. Stevia is a natural, non caloric, sweet tasting plant known for its pleasant sweet taste without leaving bitter aftertaste.  The leaves contain primarily stevioside and rebaudioside. Both have potential for  antihyperlicemic and blood pressure lowering effect....

  11. Optimization of microwave-assisted extraction of total extract, stevioside and rebaudioside-A from Stevia rebaudiana (Bertoni) leaves, using response surface methodology (RSM) and artificial neural network (ANN) modelling.

    Science.gov (United States)

    Ameer, Kashif; Bae, Seong-Woo; Jo, Yunhee; Lee, Hyun-Gyu; Ameer, Asif; Kwon, Joong-Ho

    2017-08-15

    Stevia rebaudiana (Bertoni) consists of stevioside and rebaudioside-A (Reb-A). We compared response surface methodology (RSM) and artificial neural network (ANN) modelling for their estimation and predictive capabilities in building effective models with maximum responses. A 5-level 3-factor central composite design was used to optimize microwave-assisted extraction (MAE) to obtain maximum yield of target responses as a function of extraction time (X 1 : 1-5min), ethanol concentration, (X 2 : 0-100%) and microwave power (X 3 : 40-200W). Maximum values of the three output parameters: 7.67% total extract yield, 19.58mg/g stevioside yield, and 15.3mg/g Reb-A yield, were obtained under optimum extraction conditions of 4min X 1 , 75% X 2 , and 160W X 3 . The ANN model demonstrated higher efficiency than did the RSM model. Hence, RSM can demonstrate interaction effects of inherent MAE parameters on target responses, whereas ANN can reliably model the MAE process with better predictive and estimation capabilities. Copyright © 2017. Published by Elsevier Ltd.

  12. Stevia: producción y procesamiento de un endulzante alternativo

    OpenAIRE

    Villagran Jaramillo, Alba; Huayamave Bravo, Carlos; Lara Garcia, Julio; Maluk Salem, Omar

    2009-01-01

    Stevia is a genus of about 240 species of herbs and shrubs in the sunflower family (Asteraceae), native to subtropical and tropical South America and Central America. The species Stevia rebaudiana Bertoni, commonly known as sweetleaf, sweet leaf, sugarleaf, or simply stevia, is widely grown for its sweet leaves. As a sugar substitute, stevia's taste has a slower onset and longer duration than that of sugar, although some of its extracts may have a bitter or licorice-like aftertaste at high co...

  13. Stevia preferences in Wistar rats.

    Science.gov (United States)

    Núñez Martínez, Paula; Argüelles Luis, Juan; Perillán Méndez, Carmen

    2016-11-01

    The Stevia rebaudiana plant is likely to become a major source of high-potency sweetener for the growing natural-food market. S. rebaudiana is the source of a number of sweet diterpenoid glycosides, but the major sweet constituents are rebaudioside A and stevioside. These two constituents have similar pharmacokinetic and metabolic profiles in rats and humans, and thus, studies carried out with either steviol glycoside are relevant to both. Other studies illustrate the diversity of voluntary sweet intake in mammals. This study was done using a series of two-bottle tests that compared a wide range of sweetener concentrations versus saccharin concentrations and versus water. Wistar rats displayed preferences for stevia extract and pure rebaudioside A solutions over water at a range of concentrations (0.001% to 0.3%), and their intake peak occurred at 0.1% concentration. They also preferred solutions prepared with a commercial rebaudioside A plus erythritol mixture to water, and their peak was at 2% concentration. The present study provides new information about the responses of Wistar rats to stevia compounds and commercial stevia products such as Truvia. These results could help with the appropriate dosage selection for focused behavioral and physiological studies on stevia.

  14. Stevia Glycosides : Chemical and Enzymatic Modifications of Their Carbohydrate Moieties to Improve the Sweet-Tasting Quality

    NARCIS (Netherlands)

    Gerwig, Gerrit J; Te Poele, Evelien M; Dijkhuizen, Lubbert; Kamerling, Johannis P

    2016-01-01

    Stevia glycosides, extracted from the leaves of the plant Stevia rebaudiana Bertoni, display an amazing high degree of sweetness. As processed plant products, they are considered as excellent bio-alternatives for sucrose and artificial sweeteners. Being noncaloric and having beneficial properties

  15. An efficient callus initiation and direct regeneration of Stevia ...

    African Journals Online (AJOL)

    The experiment was carried out to establish an efficient callus induction system of Stevia rebaudiana from a variety of explants as well as direct regeneration from nodes. Callus initiation was done to extract secondary metabolites. However direct regeneration is essential for rapid large-scale clonal propagation. Among leaf ...

  16. Hypoglycemic Effect of Aquatic Extract of Stevia in Pancreas of Diabetic Rats: PPAR?-dependent Regulation or Antioxidant Potential

    OpenAIRE

    Assaei, Raheleh; Mokarram, Pooneh; Dastghaib, Sanaz; Darbandi, Sara; Darbandi, Mahsa; Zal, Fatemeh; Akmali, Masoumeh; Ranjbar Omrani, Gholam Hossein

    2016-01-01

    Background: Traditional medicines with anti-diabetic effects are considered suitable supplements to treat diabetes. Among medicinal herbs, Stevia Rebaudiana Bertoni is famous for its sweet taste and beneficial effect in regulation of glucose. However, little is known about the exact mechanism of stevia in pancreatic tissue. Therefore, this study investigated the possible effects of stevia on pancreas in managing hyperglycemia seen in streptozotocin-induced Sprague-Dawley rats. Methods: Spragu...

  17. KAJIAN: KHASIAT DAN KEAMANAN STEVIA SEBAGAI PEMANIS PENGGANTI GULA

    Directory of Open Access Journals (Sweden)

    Mariana Raini

    2012-07-01

    Full Text Available The increasing of diabetic prevalence encouraged scientists to discover sweetener  as sugar substitution. Stevia is a perenial shrub (known as Stevia rebaudiana of the aster family that has been used for hundred of years to sweetening. Stevia is a natural, non caloric, sweet tasting plant known for its pleasant sweet taste without leaving bitter aftertaste.  The leaves contain primarily stevioside and rebaudioside. Both have potential for  antihyperlicemic and blood pressure lowering effect. Besides its empirical long-use without any reported toxicity in Latin America and Asia, Japan also enhanced its widespread use. Negative side effects of Stevia has not been seen yet. Stevioside and rebaudioside are not genotocsic in vitro or in vivo in animal models and  the genotocicity of steviol and some of its oxidative derivats  in vitro are not expressed in vivo. (The study is an assessment derived from 30 of the report studies/review collected from international journals. The objective is to evaluate the biochemical, to study the effect and safety of stevia herb as well as to support  its use as sugar substitution particularly for diabetic patients.  ARTIKEL Abstrak Meningkatnya prevalensi diabetes mendorong para ilmuwan untuk menemukan pemanis sebagai pengganti gula. Stevia adalah semak perenial (dikenal dengan Stevia rebaudiana bagian dari keluarga aster yang te;ah digunaka selama ratusan tahun sebagai pemanis. Stevia alami, non kalori, tanaman yang dikenal dengan rasa manisnya tanpa meninggalkan rasa pahit jika dicicipi. Daunnya mengandung stevioside dan rebaudioside. Keduanya memiliki antihpyperlicemic dan berpotensi menurunkan tekanan darah. Efek samping dari stevia belum ada. (Penelitian ini merupakan penialaian yang berasal dari 30 laporan studi/review yang dikumpulkan dari jurnal internasional. Tujuannay adalah untuk mengevaluasi biokimia, untk mempelajari efek dan kemanan Stevia serta mendukung penggunanannya sebagai pengganti gula

  18. Методологія виробництва стевії (Stevia rebaudiana) як сировини гарантованої якості

    OpenAIRE

    Кузнєцова, І. В.

    2013-01-01

    Розроблено методологію виробництва стевії(Stevia rebaudiana), дотримання якої забезпечить отримання сировини гарантованої якості. Розглянуто проблемні аспекти, які є основними при післязбиральній обробці стевії, що впливають на якість та собівартість продукції. Визначено перспективні напрями досліджень у процесі сушіння стевії Methodology of Stevia (Stevia rebaudiana), which will provide more raw material guaranteed quality. Problematic aspects that are essenti...

  19. Opposing effects of external gibberellin and Daminozide on Stevia growth and metabolites.

    Science.gov (United States)

    Karimi, Mojtaba; Hashemi, Javad; Ahmadi, Ali; Abbasi, Alireza; Pompeiano, Antonio; Tavarini, Silvia; Guglielminetti, Lorenzo; Angelini, Luciana G

    2015-01-01

    Steviol glycosides (SVglys) and gibberellins are originated from the shared biosynthesis pathway in Stevia (Stevia rebaudiana Bertoni). In this research, two experiments were conducted to study the opposing effects of external gibberellin (GA3) and Daminozide (a gibberellin inhibitor) on Stevia growth and metabolites. Results showed that GA3 significantly increased the stem length and stem dry weight in Stevia. Total soluble sugar content increased while the SVglys biosynthesis was decreased by external GA3 applying in Stevia leaves. In another experiment, the stem length was reduced by Daminozide spraying on Stevia shoots. The Daminozide did not affect the total SVglys content, while in 30 ppm concentration, significantly increased the soluble sugar production in Stevia leaves. Although the gibberellins biosynthesis pathway has previously invigorated in Stevia leaf, the Stevia response to external gibberellins implying on high precision regulation of gibberellins biosynthesis in Stevia and announces that Stevia is able to kept endogenous gibberellins in a low quantity away from SVglys production. Moreover, the assumption that the internal gibberellins were destroyed by Daminozide, lack of Daminozide effects on SVglys production suggests that gibberellins biosynthesis could not act as a competitive factor for SVglys production in Stevia leaves.

  20. Influence of sweetener stevia on the quality of strawberry flavoured fresh yoghurt

    Directory of Open Access Journals (Sweden)

    Katarina Lisak

    2011-09-01

    Full Text Available Stevia is a natural, non energetic sweetener, 200-300 times sweeter than sucrose, and is obtained by leaves extraction of the Stevia rebaudiana. According to the low energy value of stevia to sweeten food products, it is a great possibility of its use in the dairy industry. This study examined the differences in the sweetness of the strawberry flavoured yoghurt with the addition of sucrose, stevia and equal portions of sucrose and stevia; each combination was used at three different concentrations (3, 4.5 and 6 % / 100 g yoghurt. Stevia was diluted in a concentration which, according to the literature, matches the sweetness profile of sucrose. Viscosity of the yoghurts was determined by the rheometer, and sensory profiling of the products was evaluated by a panel using the ranking test and weighted factors methods. The level of sweetness of all yoghurt samples (using sucrose, stevia and mixture of sucrose and stevia was judged by a test panel, and products were rated in the terms of degree of sweetness as sucrose > sucrose + stevia > stevia. The recommended level by panelists of any type or combination of sweeteners for strawberry yoghurt was 4.5 g sweetner/100 g. The apparent viscosity was lower in sucrose yoghurts compared to products made with stevia or stevia + sucrose which was also reflected in the sensory scores.

  1. Avaliação do efeito do polieletrólito aniônico na clarificação do extrato aquoso de Stevia rebaudiana Evaluation of anionic polyelectrolyte effect in the clarification of the Stevia rebaudiana aqueous extract

    Directory of Open Access Journals (Sweden)

    Élcio J. Bunhak

    2004-12-01

    Full Text Available A clarificação é uma etapa relevante no processo de obtenção de adoçantes a base de esteviosídeo. Neste trabalho, utilizou-se o polieletrólito aniônico (BETZDEARBORN TM F11 na decantação da suspensão do extrato cru, que possui cor escura, e avaliou-se sua aplicabilidade. O extrato utilizado era obtido a partir de plantas selecionadas e modificadas geneticamente, no Núcleo de Estudos em Produtos Naturais (NEPRON no Universidade Estadual de Maringá (UEM, o qual mantém uma linha de pesquisa denominada Projeto Stevia e planta piloto em parceria com a iniciativa privada. Os ensaios foram realizados em aparelho Jar Test divididos em duas etapas, que se distinguiam na dosagem do polímero e na utilização do sobrenadante ou da suspensão total após tratamento com óxido de cálcio, o qual realizava o ajuste do pH. Realizou-se leituras de absorbâncias em 420 (cor e 670etam (turbidez e, fez-se cálculos de percentagem de despigmentação e de velocidade de decantação. Os melhores resultados na etapa 1, onde se aplicou baixas quantidades de polieletrólito aniônico e utilizou-se o sobrenadante, foram 72,2 e 97,0% para despigmentação em 420etam e 670etam, respectivamente e velocidade de decantação de 0,72cm/min. Já, na etapa 2, onde elevou-se a quantidade do polieletrólito e utilizou-se a suspensão total, os melhores resultados obtidos foram de 70,5% de despigmentação em 420etam, 85,6% de despigmentação em 670hm e velocidade de decantação de 0,71cm/min.The clarification is an important stage in the process of sweeteners for obtaining the stevioside. In this work, it was used the anionic polyelectrolyte (BETZDEARBORN TM F11 for decantation of the raw extract suspension, which possesses dark color, and it was evaluated its sweats application. The used extract was obtained in first stage from selected plants and modified genetically, in the Nucleus of Studies in Natural Products of the Universidade Estadual de Maringá (UEM

  2. Use of just-about-right scales and penalty analysis to determine appropriate concentrations of stevia sweeteners for vanilla yogurt.

    Science.gov (United States)

    Narayanan, P; Chinnasamy, B; Jin, L; Clark, S

    2014-01-01

    With the mainstream emergence of natural sweeteners such as stevia, which is available in different commercial formulations, suitability for yogurt needs to be validated. The present study aimed to determine the appropriate concentration level of 3 processed stevia sweeteners/supplements in commercial plain low-fat yogurt flavored with natural vanilla. Three different levels of sucrose, aspartame, an erythritol and 95% rebaudiana A stevia sweetener, a 95% pure mix of maltodextrin and steviol glycosides, and a cold water stevia extract were used in the study. The just-about-right level for each sweetener and consumer acceptability of each naturally flavored low-fat vanilla yogurt were evaluated. Results from penalty analysis demonstrated that only 0.7% of stevia containing maltodextrin and 95% steviol glycoside was necessary, whereas higher levels (between 4.0 to 5.5%) were more appropriate for stevia containing erythritol and 95% rebaudiana A or cold water extract of stevia, respectively. The concentrations of stevia sweeteners used influenced the perceived sweetness and sourness. In general, consumers disliked the yogurt sweetened with stevia or aspartame, and neither disliked nor liked the yogurt sweetened with sucrose, which was largely driven by perceived sourness of the base yogurt. The findings underline the importance of careful selection of stevia type and concentration as well as optimizing yogurt cultures and fermentation conditions before product launch. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  3. Stevia : A True Glycoside Used as a Sweetener and Not Affecting Behavior

    OpenAIRE

    Marie-Claire Cammaerts, Axel Dero, Roger Cammaerts

    2016-01-01

    Using ants as models, the glycoside rebaudioside A, a sweetener extracted from the plant Stevia rebaudiana and commercialized under the name ‘stevia’, was shown to have no effect on their food consumption, locomotion, precision of reaction, response to pheromones, brood caring, cognition, visual and olfactory conditioning and memory, although this sweetener slightly increased the ants audacity. However, when having the choice between stevia and saccharose, the ants somewhat preferred the latt...

  4. ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF STEVIA LEAVES EXTRACTS AND SILVER NANOPARTICLES COLLOIDS

    Directory of Open Access Journals (Sweden)

    Iryna Laguta

    2016-12-01

    Full Text Available Three extracts of Stevia rebaudiana (Bertoni were prepared using various types of raw materials: leaves of plants grown ex situ, leaves of plants grown in vitro, callus culture formed on damaged leaves. Composition of the extracts, their activity in the synthesis of silver nanoparticles colloids, as well as antioxidant and antimicrobial properties of the extracts and the colloids were investigated.

  5. Steviol glycosides from Stevia: biosynthesis pathway review and their application in foods and medicine.

    Science.gov (United States)

    Yadav, Sudesh Kumar; Guleria, Praveen

    2012-01-01

    Stevia rebaudiana, a perennial herb from the Asteraceae family, is known to the scientific world for its sweetness and steviol glycosides (SGs). SGs are the secondary metabolites responsible for the sweetness of Stevia. They are synthesized by SG biosynthesis pathway operating in the leaves. Most of the genes encoding the enzymes of this pathway have been cloned and characterized from Stevia. Out of various SGs, stevioside and rebaudioside A are the major metabolites. SGs including stevioside have also been synthesized by enzymes and microbial agents. These are non-mutagenic, non-toxic, antimicrobial, and do not show any remarkable side-effects upon consumption. Stevioside has many medical applications and its role against diabetes is most important. SGs have made Stevia an important part of the medicinal world as well as the food and beverage industry. This article presents an overview on Stevia and the importance of SGs.

  6. Factors affecting microcuttings of Stevia using a mist-chamber propagation box.

    Science.gov (United States)

    Osman, Mohamad; Samsudin, Nur Syamimi; Faruq, Golam; Nezhadahmadi, Arash

    2013-01-01

    Stevia rebaudiana Bertoni is a member of Compositae family. Stevia plant has zero calorie content and its leaves are estimated to be 300 times sweeter than sugar. This plant is believed to be the most ideal substitute for sugar and important to assist in medicinal value especially for diabetic patients. In this study, microcutting techniques using a mist-chamber propagation box were used as it was beneficial for propagation of Stevia and gave genetic uniformity to the plant. The effects of different treatments on root stimulation of Stevia in microcuttings technique were evaluated. Treatments studied were different sizes of shoot cuttings, plant growth regulators, lights, and shades. Data logger was used to record the mean value of humidity (>90% RH), light intensity (673-2045 lx), and temperature (28.6-30.1°C) inside the mist-chamber propagation box. From analysis of variance, there were significant differences between varieties and treatments in parameters studied (P Stevia.

  7. Purificação do extrato aquoso de Stevia rebaudiana Bertoni através dos processos com zeólitas e membranas - DOI: 10.4025/actascitechnol.v26i1.1546

    Directory of Open Access Journals (Sweden)

    Suzana Lemansky Rezende

    2004-03-01

    Full Text Available Neste trabalho utilizaram-se os seguintes processos de purificação do extrato aquoso de stevia a 10%: adsorção com zeólita CaX modificada por troca iônica, separação por ultrafiltração tangencial com membranas cerâmicas e o processo acoplado, zeólitas e membrana. Os melhores resultados de clarificação foram obtidos com o processo acoplado, empregando-se adsorção em leitos de zeólita CaX por 12 horas seguido da ultrafiltração em membrana cerâmica de tamanho médio dos poros 0,05 µm e pressão transmembrana de 4 bar. A clarificação final, após 2,5 horas de filtragem chegou a 99,56%. Esses resultados mostram que o processo acoplado, zeólitas e membrana, é promissor na purificação do extrato bruto de stevia. Porém o rendimento dos adoçantes (esteviosídeo e rebaudosídeo foi superior quando se trabalhou com o processo isolado com membranas

  8. Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste

    NARCIS (Netherlands)

    Te Poele, Evelien M; Devlamynck, Tim; Jäger, Manuel; Gerwig, Gerrit J; Van de Walle, Davy; Dewettinck, Koen; Hirsch, Anna K H; Kamerling, Johannis P; Soetaert, Wim; Dijkhuizen, Lubbert

    2018-01-01

    Steviol glycosides from the leaves of the plant Stevia rebaudiana are high-potency natural sweeteners but suffer from a lingering bitterness. The Lactobacillus reuteri 180 wild-type glucansucrase Gtf180-ΔN, and in particular its Q1140E-mutant, efficiently α-glucosylated rebaudioside A (RebA), using

  9. Effect of partial replacement of sugar with stevia on the quality of kulfi.

    Science.gov (United States)

    Giri, Apurba; Rao, H G Ramachandra; V, Ramesh

    2014-08-01

    Stevia is a natural sweetener obtained from the leaf of Stevia rebaudiana plant. Its refined extract powder is 130-300 times sweeter than sucrose. Besides, it prevents diabetes, decreases weight, prevents tooth decay, increases digestion etc. Dietetic kulfi was produced, in which 50, 60 and 70% sugar was replaced with 0.05, 0.06 and 0.07% refined stevia extract powder respectively. At higher levels of sugar replacement there was a significant decrease in specific gravity, melting rate, carbohydrate percentage and total calorie content and a significant increase in freezing point, hardness and fat, protein, ash and moisture percentage. Kulfi prepared by replacing half the sugar content with stevia was adjudged on par with the control in sensory characteristics. Above 50% sugar replacement resulted in bitterness, lack of brownish appearance and presence of icy texture.

  10. Electrophysiological, vasoactive, and gastromodulatory effects of stevia in healthy Wistar rats.

    Science.gov (United States)

    Yesmine, Saquiba; Connolly, Kylie; Hill, Nicholas; Coulson, Fiona R; Fenning, Andrew S

    2013-07-01

    Antihypertensive and antidiabetic effects of stevia, Stevia rebaudiana (Asteraceae), have been demonstrated in several human and animal models. The current study aims to define stevia's role in modifying the electrophysiological and mechanical properties of cardiomyocytes, blood vessels, and gastrointestinal smooth muscle. Tissues from thoracic aorta, mesenteric arteries, ileum, and left ventricular papillary muscles were excised from 8-week-old healthy Wistar rats. The effects of stevia (1 × 10-9 M to 1 × 10-4 M) were measured on these tissues. Stevia's effects in the presence of verapamil, 4-AP, and L-NAME were also assessed. In cardiomyocytes, stevia attenuated the force of contraction, decreased the average peak amplitude, and shortened the repolarisation phase of action potential - repolarisation phase of action potential20 by 25 %, repolarisation phase of action potential50 by 34 %, and repolarisation phase of action potential90 by 36 %. Stevia caused relaxation of aortic tissues which was significantly potentiated in the presence of verapamil. In mesenteric arteries, incubation with L-NAME failed to block stevia-induced relaxation indicating the mechanism of action may not be fully via nitric oxide-dependent pathways. Stevia concentration-dependently reduced electrical field stimulated and carbachol-induced contractions in the isolated ileum. This study is the first to show the effectiveness of stevia in reducing cardiac action potential duration at 20 %, 50 %, and 90 % of repolarisation. Stevia also showed beneficial modulatory effects on cardiovascular and gastrointestinal tissues via calcium channel antagonism, activation of the M2 muscarinic receptor function, and enhanced nitric oxide release. Georg Thieme Verlag KG Stuttgart · New York.

  11. Stevia and Saccharin Preferences in Rats and Mice

    Science.gov (United States)

    Bahrani, Mahsa; Zukerman, Steven; Ackroff, Karen

    2010-01-01

    Use of natural noncaloric sweeteners in commercial foods and beverages has expanded recently to include compounds from the plant Stevia rebaudiana. Little is known about the responses of rodents, the animal models for many studies of taste systems and food intake, to stevia sweeteners. In the present experiments, preferences of female Sprague–Dawley rats and C57BL/6J mice for different stevia products were compared with those for the artificial sweetener saccharin. The stevia component rebaudioside A has the most sweetness and least off-tastes to human raters. In ascending concentration tests (48-h sweetener vs. water), rats and mice preferred a high-rebaudioside, low-stevioside extract as strongly as saccharin, but the extract stimulated less overdrinking and was much less preferred to saccharin in direct choice tests. Relative to the extract, mice drank more pure rebaudioside A and showed stronger preferences but still less than those for saccharin. Mice also preferred a commercial mixture of rebaudioside A and erythritol (Truvia). Similar tests of sweet receptor T1R3 knockout mice and brief-access licking tests with normal mice suggested that the preferences were based on sweet taste rather than post-oral effects. The preference response of rodents to stevia sweeteners is notable in view of their minimal response to some other noncaloric sweeteners (aspartame and cyclamate). PMID:20413452

  12. Replacing sugar with S. rebaudiana extracts on the physicochemical and sensory properties of strawberry ice cream

    Directory of Open Access Journals (Sweden)

    Irma Aranda-Gonzalez

    2016-04-01

    Full Text Available ABSTRACT: Ice cream is a product whose formulation requires considerable amounts of sugar. In addition to providing flavor, sugar contributes to the physicochemical characteristics of ice cream but its consumption in large quantities is related to chronic diseases such as diabetes and obesity. For this reason, the food industry seeks to formulate products with sweeteners that preserve the physicochemical and sensory characteristics of the original product. Stevia rebaudiana is a plant that naturally contains glycosides with no calories and high sweetening capacity and it is considered safe for consumption. Therefore the aim of this research was to evaluate the effect of replacing sugar with different levels of extracts of S. rebaudiana Morita II and Criolla, on the physicochemical and sensory properties of strawberry ice cream. Using a 2x2 factorial experimental design, a total of 4 formulations were prepared with two levels of concentration of the aqueous extract of Stevia rebaudiana (5 or 8% and the variety of S. rebaudiana (Morita II or Criolla. Proximate composition, physicochemical properties and sensory evaluation were determined in processed products. The proximate analysis of strawberry ice cream varied significantly (P<0.05 depending of variety and level used on the formulation. The viscosities of all ice cream mixes were decreasing as the shear rate was increased, indicating a pseudoplastic behavior. The sensory analysis showed differences (P<0.05 among the formulations tested, however the score of all products were above the indifference point, suggesting that all of these formulations may have a commercial potential.

  13. Diterpene glycosides from Stevia phlebophylla A. Gray.

    Science.gov (United States)

    Ceunen, Stijn; Wim, De Borggraeve; Compernolle, Frans; Mai, Anh Hung; Geuns, Jan M C

    2013-09-20

    The rare Mexican species Stevia phlebophylla A. Gray was long considered to be the only known Stevia species, beside the well-known S. rebaudiana, containing the highly sweet diterpenoid steviol glycosides. We report a re-evaluation of this claim after phytochemically screening leaves obtained from two herbarium specimens of S. phlebophylla for the presence of steviol glycosides. Despite extensive MS analyses, no steviol glycosides could be unambiguously verified. Instead, the main chromatographic peak eluting at retention times similar to those of steviol glycosides was identified as a new compound, namely 16β-hydroxy-17-acetoxy-ent-kauran-19-oic acid-(6-O-β-D-xylopyranosyl-β-D-glucopyranosyl) ester (1) on the basis of extensive NMR and MS data as well as the characterization of its acid hydrolysate. Seven more compounds were detected by ESIMS which are possibly structurally related to 1. It can therefore be concluded that S. phlebophylla is unlikely to contain significant amounts of steviol glycosides, if any. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. Hypoglycemic Effect of Aquatic Extract of Stevia in Pancreas of Diabetic Rats: PPARγ-dependent Regulation or Antioxidant Potential.

    Science.gov (United States)

    Assaei, Raheleh; Mokarram, Pooneh; Dastghaib, Sanaz; Darbandi, Sara; Darbandi, Mahsa; Zal, Fatemeh; Akmali, Masoumeh; Ranjbar Omrani, Gholam Hossein

    2016-01-01

    Traditional medicines with anti-diabetic effects are considered suitable supplements to treat diabetes. Among medicinal herbs, Stevia Rebaudiana Bertoni is famous for its sweet taste and beneficial effect in regulation of glucose. However, little is known about the exact mechanism of stevia in pancreatic tissue. Therefore, this study investigated the possible effects of stevia on pancreas in managing hyperglycemia seen in streptozotocin-induced Sprague-Dawley rats. Sprague-Dawley rats were divided into four groups including normoglycemic, diabetic and two more diabetic groups in which, one was treated with aquatic extract of stevia (400 mg/kg) and the other with pioglitazone (10 mg/kg) for the period of 28 days. After completion of the experimental duration, rats were dissected; blood samples and pancreas were further used for detecting biochemical and histopathological changes. FBS, TG, cholestrol, HDL, LDL, ALT and AST levels were measured in sera. Moreover, MDA (malondialdehyde) level, catalase activity, levels of insulin and PPARγ mRNA expression were also measured in pancreatic tissue. Aquatic extract of stevia significantly reduced the FBS, triglycerides, MDA, ALT, AST levels and normalized catalase activity in treated rats compared with diabetic rats (pstevia surprisingly, increased PPARγ and insulin mRNA levels in treated rats (pstevia compensated for the histopathological damage in diabetic rats. It is concluded that stevia acts on pancreatic tissue to elevate the insulin level and exerts beneficial anti-hyperglycemic effects through the PPARγ-dependent mechanism and stevia's antioxidant properties.

  15. Microbial production of next-generation stevia sweeteners.

    Science.gov (United States)

    Olsson, Kim; Carlsen, Simon; Semmler, Angelika; Simón, Ernesto; Mikkelsen, Michael Dalgaard; Møller, Birger Lindberg

    2016-12-07

    The glucosyltransferase UGT76G1 from Stevia rebaudiana is a chameleon enzyme in the targeted biosynthesis of the next-generation premium stevia sweeteners, rebaudioside D (Reb D) and rebaudioside M (Reb M). These steviol glucosides carry five and six glucose units, respectively, and have low sweetness thresholds, high maximum sweet intensities and exhibit a greatly reduced lingering bitter taste compared to stevioside and rebaudioside A, the most abundant steviol glucosides in the leaves of Stevia rebaudiana. In the metabolic glycosylation grid leading to production of Reb D and Reb M, UGT76G1 was found to catalyze eight different reactions all involving 1,3-glucosylation of steviol C 13 - and C 19 -bound glucoses. Four of these reactions lead to Reb D and Reb M while the other four result in formation of side-products unwanted for production. In this work, side-product formation was reduced by targeted optimization of UGT76G1 towards 1,3 glucosylation of steviol glucosides that are already 1,2-diglucosylated. The optimization of UGT76G1 was based on homology modelling, which enabled identification of key target amino acids present in the substrate-binding pocket. These residues were then subjected to site-saturation mutagenesis and a mutant library containing a total of 1748 UGT76G1 variants was screened for increased accumulation of Reb D or M, as well as for decreased accumulation of side-products. This screen was performed in a Saccharomyces cerevisiae strain expressing all enzymes in the rebaudioside biosynthesis pathway except for UGT76G1. Screening of the mutant library identified mutations with positive impact on the accumulation of Reb D and Reb M. The effect of the introduced mutations on other reactions in the metabolic grid was characterized. This screen made it possible to identify variants, such as UGT76G1 Thr146Gly and UGT76G1 His155Leu , which diminished accumulation of unwanted side-products and gave increased specific accumulation of the desired

  16. Factors Affecting Microcuttings of Stevia Using a Mist-Chamber Propagation Box

    Directory of Open Access Journals (Sweden)

    Mohamad Osman

    2013-01-01

    Full Text Available Stevia rebaudiana Bertoni is a member of Compositae family. Stevia plant has zero calorie content and its leaves are estimated to be 300 times sweeter than sugar. This plant is believed to be the most ideal substitute for sugar and important to assist in medicinal value especially for diabetic patients. In this study, microcutting techniques using a mist-chamber propagation box were used as it was beneficial for propagation of Stevia and gave genetic uniformity to the plant. The effects of different treatments on root stimulation of Stevia in microcuttings technique were evaluated. Treatments studied were different sizes of shoot cuttings, plant growth regulators, lights, and shades. Data logger was used to record the mean value of humidity (>90% RH, light intensity (673–2045 lx, and temperature (28.6–30.1°C inside the mist-chamber propagation box. From analysis of variance, there were significant differences between varieties and treatments in parameters studied (P<0.05. For the size of shoot cuttings treatment, 6 nodes cuttings were observed to increase root number. As compared to control, shoot cuttings treated with indole butyric acid (IBA had better performance regarding root length. Yellow light and 50% shade treatments showed higher root and leaf number and these conditions can be considered as crucial for potential propagation of Stevia.

  17. Factors Affecting Microcuttings of Stevia Using a Mist-Chamber Propagation Box

    Science.gov (United States)

    Osman, Mohamad; Samsudin, Nur Syamimi; Faruq, Golam

    2013-01-01

    Stevia rebaudiana Bertoni is a member of Compositae family. Stevia plant has zero calorie content and its leaves are estimated to be 300 times sweeter than sugar. This plant is believed to be the most ideal substitute for sugar and important to assist in medicinal value especially for diabetic patients. In this study, microcutting techniques using a mist-chamber propagation box were used as it was beneficial for propagation of Stevia and gave genetic uniformity to the plant. The effects of different treatments on root stimulation of Stevia in microcuttings technique were evaluated. Treatments studied were different sizes of shoot cuttings, plant growth regulators, lights, and shades. Data logger was used to record the mean value of humidity (>90% RH), light intensity (673–2045 lx), and temperature (28.6–30.1°C) inside the mist-chamber propagation box. From analysis of variance, there were significant differences between varieties and treatments in parameters studied (P Stevia. PMID:24470797

  18. Hypoglycemic Effect of Aquatic Extract of Stevia in Pancreas of Diabetic Rats: PPARγ-dependent Regulation or Antioxidant Potential

    Science.gov (United States)

    Assaei, Raheleh; Mokarram, Pooneh; Dastghaib, Sanaz; Darbandi, Sara; Darbandi, Mahsa; Zal, Fatemeh; Akmali, Masoumeh; Ranjbar Omrani, Gholam Hossein

    2016-01-01

    Background: Traditional medicines with anti-diabetic effects are considered suitable supplements to treat diabetes. Among medicinal herbs, Stevia Rebaudiana Bertoni is famous for its sweet taste and beneficial effect in regulation of glucose. However, little is known about the exact mechanism of stevia in pancreatic tissue. Therefore, this study investigated the possible effects of stevia on pancreas in managing hyperglycemia seen in streptozotocin-induced Sprague-Dawley rats. Methods: Sprague-Dawley rats were divided into four groups including normoglycemic, diabetic and two more diabetic groups in which, one was treated with aquatic extract of stevia (400 mg/kg) and the other with pioglitazone (10 mg/kg) for the period of 28 days. After completion of the experimental duration, rats were dissected; blood samples and pancreas were further used for detecting biochemical and histopathological changes. FBS, TG, cholestrol, HDL, LDL, ALT and AST levels were measured in sera. Moreover, MDA (malondialdehyde) level, catalase activity, levels of insulin and PPARγ mRNA expression were also measured in pancreatic tissue. Results: Aquatic extract of stevia significantly reduced the FBS, triglycerides, MDA, ALT, AST levels and normalized catalase activity in treated rats compared with diabetic rats (pstevia surprisingly, increased PPARγ and insulin mRNA levels in treated rats (pstevia compensated for the histopathological damage in diabetic rats. Conclusion: It is concluded that stevia acts on pancreatic tissue to elevate the insulin level and exerts beneficial anti-hyperglycemic effects through the PPARγ-dependent mechanism and stevia’s antioxidant properties. PMID:27141265

  19. Pretreatment with ethanol as an alternative to improve steviol glycosides extraction and purification from a new variety of stevia.

    Science.gov (United States)

    Formigoni, Maysa; Milani, Paula Gimenez; da Silva Avíncola, Alexandre; Dos Santos, Vanessa Jorge; Benossi, Livia; Dacome, Antônio Sergio; Pilau, Eduardo Jorge; da Costa, Silvio Claudio

    2018-02-15

    Leaves of a new variety of Stevia rebaudiana with a high content of rebaudioside A were pretreated with ethanol. The ethanolic extract showed high antioxidant potential and 39 compounds were identified, by UPLC/HRMS, among them one not yet mentioned in the literature for stevia leaves. From the in natura leaves and pretreated leaves, the conditions of aqueous extraction of steviol glycosides were investigated using response surface methodology. The aqueous extracts obtained were purified by ion exchange chromatography techniques and membrane separation methods. The recuperation of steviol glycosides was 4.02g for pretreated leaves and 2.20g for in natura leaves. The level of purity was, respectively, 87% and 84.8%. The results obtained demonstrate that pretreatment increases the yield and purity level of stevia sweeteners by the use of environmentally friendly methodologies and the final product presented acceptable sensory characteristics. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Development of Next Generation Stevia Sweetener: Rebaudioside M

    Directory of Open Access Journals (Sweden)

    Indra Prakash

    2014-02-01

    Full Text Available This work aims to review and showcase the unique properties of rebaudioside M as a natural non-caloric potential sweetener in food and beverage products. To determine the potential of rebaudioside M, isolated from Stevia rebaudiana Bertoni, as a high potency sweetener, we examined it with the Beidler Model. This model estimated that rebaudioside M is 200–350 times more potent than sucrose. Numerous sensory evaluations of rebaudioside M’s taste attributes illustrated that this steviol glycoside possesses a clean, sweet taste with a slightly bitter or licorice aftertaste. The major reaction pathways in aqueous solutions (pH 2–8 for rebaudioside M are similar to rebaudioside A. Herein we demonstrate that rebaudioside M could be of great interest to the global food industry because it is well-suited for blending and is functional in a wide variety of food and beverage products.

  1. Development of Next Generation Stevia Sweetener: Rebaudioside M

    Science.gov (United States)

    Prakash, Indra; Markosyan, Avetik; Bunders, Cynthia

    2014-01-01

    This work aims to review and showcase the unique properties of rebaudioside M as a natural non-caloric potential sweetener in food and beverage products. To determine the potential of rebaudioside M, isolated from Stevia rebaudiana Bertoni, as a high potency sweetener, we examined it with the Beidler Model. This model estimated that rebaudioside M is 200–350 times more potent than sucrose. Numerous sensory evaluations of rebaudioside M’s taste attributes illustrated that this steviol glycoside possesses a clean, sweet taste with a slightly bitter or licorice aftertaste. The major reaction pathways in aqueous solutions (pH 2–8) for rebaudioside M are similar to rebaudioside A. Herein we demonstrate that rebaudioside M could be of great interest to the global food industry because it is well-suited for blending and is functional in a wide variety of food and beverage products. PMID:28234311

  2. Effects of stevia on synaptic plasticity and NADPH oxidase level of CNS in conditions of metabolic disorders caused by fructose.

    Science.gov (United States)

    Chavushyan, V A; Simonyan, K V; Simonyan, R M; Isoyan, A S; Simonyan, G M; Babakhanyan, M A; Hovhannisyian, L E; Nahapetyan, Kh H; Avetisyan, L G; Simonyan, M A

    2017-12-19

    Excess dietary fructose intake associated with metabolic syndrome and insulin resistance and increased risk of developing type 2 diabetes. Previous animal studies have reported that diabetic animals have significantly impaired behavioural and cognitive functions, pathological synaptic function and impaired expression of glutamate receptors. Correction of the antioxidant status of laboratory rodents largely prevents the development of fructose-induced plurimetabolic changes in the nervous system. We suggest a novel concept of efficiency of Stevia leaves for treatment of central diabetic neuropathy. By in vivo extracellular studies induced spike activity of hippocampal neurons during high frequency stimulation of entorhinal cortex, as well as neurons of basolateral amygdala to high-frequency stimulation of the hippocampus effects of Stevia rebaudiana Bertoni plant evaluated in synaptic activity in the brain of fructose-enriched diet rats. In the conditions of metabolic disorders caused by fructose, antioxidant activity of Stevia rebaudiana was assessed by measuring the NOX activity of the hippocampus, amygdala and spinal cord. In this study, the characteristic features of the metabolic effects of dietary fructose on synaptic plasticity in hippocampal neurons and basolateral amygdala and the state of the NADPH oxidase (NOX) oxidative system of these brain formations are revealed, as well as the prospects for development of multitarget and polyfunctional phytopreparations (with adaptogenic, antioxidant, antidiabetic, nootropic activity) from native raw material of Stevia rebaudiana. Stevia modulates degree of expressiveness of potentiation/depression (approaches but fails to achieve the norm) by shifting the percentage balance in favor of depressor type of responses during high-frequency stimulation, indicating its adaptogenic role in plasticity of neural networks. Under the action of fructose an increase (3-5 times) in specific quantity of total fraction of NOX

  3. Comparative Transcriptomics Unravel Biochemical Specialization of Leaf Tissues of Stevia for Diterpenoid Production.

    Science.gov (United States)

    Kim, Mi Jung; Jin, Jingjing; Zheng, Junshi; Wong, Limsoon; Chua, Nam-Hai; Jang, In-Cheol

    2015-12-01

    Stevia (Stevia rebaudiana) produces not only a group of diterpenoid glycosides known as steviol glycosides (SGs), but also other labdane-type diterpenoids that may be spatially separated from SGs. However, their biosynthetic routes and spatial distribution in leaf tissues have not yet been elucidated. Here, we integrate metabolome and transcriptome analyses of Stevia to explore the biosynthetic capacity of leaf tissues for diterpenoid metabolism. Tissue-specific chemical analyses confirmed that SGs were accumulated in leaf cells but not in trichomes. On the other hand, Stevia leaf trichomes stored other labdane-type diterpenoids such as oxomanoyl oxide and agatholic acid. RNA sequencing analyses from two different tissues of Stevia provided a comprehensive overview of dynamic metabolic activities in trichomes and leaf without trichomes. These metabolite-guided transcriptomics and phylogenetic and gene expression analyses clearly identified specific gene members encoding enzymes involved in the 2-C-methyl-d-erythritol 4-phosphate pathway and the biosynthesis of steviol or other labdane-type diterpenoids. Additionally, our RNA sequencing analysis uncovered copalyl diphosphate synthase (SrCPS) and kaurene synthase1 (SrKS1) homologs, SrCPS2 and KS-like (SrKSL), which were specifically expressed in trichomes. In vitro and in planta assays showed that unlike SrCPS and SrKS1, SrCPS2 synthesized labda-13-en-8-ol diphosphate and successively catalyzed the formation of manoyl oxide and epi-manoyl oxide in combination with SrKSL. Our findings suggest that Stevia may have evolved to use distinct metabolic pathways to avoid metabolic interferences in leaf tissues for efficient production of diverse secondary metabolites. © 2015 American Society of Plant Biologists. All Rights Reserved.

  4. Comparative Transcriptomics Unravel Biochemical Specialization of Leaf Tissues of Stevia for Diterpenoid Production1

    Science.gov (United States)

    Kim, Mi Jung; Jin, Jingjing; Zheng, Junshi

    2015-01-01

    Stevia (Stevia rebaudiana) produces not only a group of diterpenoid glycosides known as steviol glycosides (SGs), but also other labdane-type diterpenoids that may be spatially separated from SGs. However, their biosynthetic routes and spatial distribution in leaf tissues have not yet been elucidated. Here, we integrate metabolome and transcriptome analyses of Stevia to explore the biosynthetic capacity of leaf tissues for diterpenoid metabolism. Tissue-specific chemical analyses confirmed that SGs were accumulated in leaf cells but not in trichomes. On the other hand, Stevia leaf trichomes stored other labdane-type diterpenoids such as oxomanoyl oxide and agatholic acid. RNA sequencing analyses from two different tissues of Stevia provided a comprehensive overview of dynamic metabolic activities in trichomes and leaf without trichomes. These metabolite-guided transcriptomics and phylogenetic and gene expression analyses clearly identified specific gene members encoding enzymes involved in the 2-C-methyl-d-erythritol 4-phosphate pathway and the biosynthesis of steviol or other labdane-type diterpenoids. Additionally, our RNA sequencing analysis uncovered copalyl diphosphate synthase (SrCPS) and kaurene synthase1 (SrKS1) homologs, SrCPS2 and KS-like (SrKSL), which were specifically expressed in trichomes. In vitro and in planta assays showed that unlike SrCPS and SrKS1, SrCPS2 synthesized labda-13-en-8-ol diphosphate and successively catalyzed the formation of manoyl oxide and epi-manoyl oxide in combination with SrKSL. Our findings suggest that Stevia may have evolved to use distinct metabolic pathways to avoid metabolic interferences in leaf tissues for efficient production of diverse secondary metabolites. PMID:26438788

  5. Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinent

    Science.gov (United States)

    Chester, Karishma; Tamboli, Ennus T.; Singh, Mhaveer; Ahmad, Sayeed

    2012-01-01

    Background: A high performance thin layer chromatographic (HPTLC) method was developed for simultaneous estimation of stevioside and rebaudioside A in Stevia rebaudiana samples collected from different regions of Indian subcontinent. Materials and Methods: The separation was achieved by using acetone: ethyl acetate: water (5:4:1, v/v/v) as the solvent system on precoated silica gel 60 F254 TLC plates. The densitometric quantification of stevia glycosides was carried out at wavelength 360 nm in absorption mode after spraying with anisaldehyde sulphuric acid as detecting reagent. Results: The well resolved peaks for stevioside and rebaudioside A were observed at Rf values 0.31± 0.02 and 0.21± 0.02 respectively. The calibration curves were found linear with a wide range of concentration 100 - 2000 ng spot-1 with good correlation coefficient 0.996 and 0.991 for stevioside and rebaudioside A, respectively. Conclusions: The proposed method was validated as per the ICH (International Conferences on Harmonization) guidelines and found simple, sensitive, economic, reproducible, robust and accurate for quantitative analysis of stevia glycosides, which can be applied for quality control of stevia as well as to check. PMID:23248559

  6. Characterization of Stevia leaves by LC-QTOF MS/MS analysis of polar and non-polar extracts.

    Science.gov (United States)

    Molina-Calle, M; Priego-Capote, F; Luque de Castro, M D

    2017-03-15

    Stevia is currently a well-known plant thanks to its sweeting power. Numerous studies that elucidate its composition were exclusively focused on determination of steviol and its glycosides. Untargeted analysis was applied to obtain a profile of main compounds present in extracts from Stevia (Stevia rebaudiana Bertoni) leaves using LC-MS in high resolution mode with a quadrupole-time of flight analyzer. Eighty-nine compounds were tentatively identified and classified into different families: flavonoids; quinic and caffeic acids and derivatives; diterpenoids (including steviol and glycosides); sesquiterpenoids; amino acids and derivatives; fatty amides and derivatives; fatty acids and derivatives; oligosaccharides; glycerolipids; purines; and retinoids. New steviol glycosides were tentatively identified and their possible structures proposed. Other compounds were tentatively identified in Stevia for the first time, such as fatty acid amides. These results reveal the wide range of compounds present in Stevia, which could be responsible for the nutraceutical effects ascribed to their leaves. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. [Stevia in the fight against dental caries].

    Science.gov (United States)

    Ma, M S; Blanksma, N G

    2015-01-01

    Stevia is a natural, non-caloric sweetener of plant origin. The sweetening power of stevia is several hundred times larger than that of table sugar (sucrose). On the basis of available research, the European Food Safety Authority concluded that stevia is safe for human consumption. Since then, stevia has been approved as a sweetener for the European market. As a substitute for sucrose, stevia can contribute to a reduced caloric intake and can play a role in the prevention and/or treatment of metabolic disorders. In addition, stevia is non-cariogenic and is, moreover, affordable. Promoting the consumption of stevia can therefore be a preventive means of fighting dental caries.

  8. Comparison of reduced sugar high quality chocolates sweetened with stevioside and crude stevia 'green' extract.

    Science.gov (United States)

    Torri, Luisa; Frati, Alessandra; Ninfali, Paolino; Mantegna, Stefano; Cravotto, Giancarlo; Morini, Gabriella

    2017-06-01

    The demand for zero and reduced-sugar food products containing cocoa is expanding continuously. The present study was designed to evaluate the feasibility of producing high-quality chocolate sweetened with a crude extract of Stevia rebaudiana (Bertoni) prepared by a green microwave-assisted water-steam extraction procedure. Seven approximately isosweet chocolate formulations were developed, mixing cocoa paste, sucrose, commercial stevioside, crude green extract and maltitol in different proportions. All samples were analyzed for the determination of polyphenol and flavonoid content, antioxidant activity, and sensory acceptability. The use of a crude stevia extract allowed low-sugar, high-quality chocolates to be obtained that were also acceptable by consumers and had a significant increased antioxidant activity. Moreover, consumers' segmentation revealed a cluster of consumers showing the same overall liking for the sample with 50% sucrose replaced by the stevia crude extract as that obtained with the commercial stevioside and the control sample (without sucrose replacement). The results provide information that can contribute to promoting the development of sweet food products, with advantages in terms of an improved nutritional value (reduced sugar content and increased antioxidant activity) and a reduced impact of the production process on the environment. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  9. Overexpression of a glycosyltransferase gene SrUGT74G1 from Stevia improved growth and yield of transgenic Arabidopsis by catechin accumulation.

    Science.gov (United States)

    Guleria, Praveen; Yadav, Sudesh Kumar

    2014-03-01

    Steviol glycoside and gibberellin biosynthetic routes are known as divergent branches of a common origin in Stevia. A UDP-glycosyltransferase encoded by SrUGT74G1 catalyses the conversion of steviolbioside into stevioside in Stevia rebaudiana leaves. In the present study, transgenic Arabidopsis thaliana overexpressing SrUGT74G1 cDNA from Stevia were developed to check the probability of stevioside biosynthesis in them. However, stevioside accumulation was not evident in transgenics. Also, the transgenic Arabidopsis showed no change in GA3 content on SrUGT74G1 overexpression. Surprisingly, significant accumulation of catechin was noticed in transgenics. The transgenics showed a considerable increase in shoot length, root length and rosette area. An increase in free radical scavenging activity of transgenics was noticed. Moreover, the seed yield of transgenics was also increased by 6-15% than control. Additionally, variation in trichome branching pattern on leaf surface of transgenics was observed. The trichome branching pattern was also validated by exogenous catechin exposure (10, 50, 100 ng ml(-1)) to control plants. Hence, present study reports the probable role of SrUGT74G1 from Stevia in catechin accumulation of transgenic Arabidopsis thaliana. Thus, detailed study in present perspective has revealed the role of Stevia SrUGT74G1 gene in trichome branching pattern, improved vegetative growth, scavenging potential and seed yield by catechin accumulation in transgenic Arabidopsis.

  10. Comercialización de estevia: Empresas para unos, salud para todos

    Directory of Open Access Journals (Sweden)

    María Ubaldina Cobeña Mendoza

    2012-12-01

    Full Text Available Manabi,  una de las provincias con mayor índice de problemas de diabetes. Ante el crecimiento acelerado de esta enfermedad, estudiantes de Marketing de la carrera  de Administración de Empresas de la Universidad Técnica de Manabí, buscan  la oportunidad de que se logre sembrar Estevia, produciry patentar la marca,  como  lo han  logrado productores de otros países y así paliar el problema de desempleo en el Ecuador y sobre todo comprometerse como  asesores de comercialización   de  productores  que   quisieran aprovechar las bondades y beneficios que brinda esta región con tierra fértil. La investigación revela  que en el mercado existen productos que  no rinden mayores beneficios y aún más, son perjudiciales para la salud, se los utilizan por tradición y desconocimiento  de  otras alternativas.  Para  lo cual los estudiantes,   mencionados   hicieron   un  estudio   de  mercado  sobre   la posibilidad de comercializar dicho producto, tomando como referencia una muestra de  200  personas,   que   en  un  gran  porcentaje  indicaron  estar dispuestos a cambiar sus hábitos de consumo, lo que revela la factibilidad de introducir al mercado un producto innovadory saludable como es la Estevia.

  11. Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1.

    Science.gov (United States)

    Dewitte, Griet; Walmagh, Maarten; Diricks, Margo; Lepak, Alexander; Gutmann, Alexander; Nidetzky, Bernd; Desmet, Tom

    2016-09-10

    UDP-glycosyltransferases (UGTs) are a promising class of biocatalysts that offer a sustainable alternative for chemical glycosylation of natural products. In this study, we aimed to characterize plant-derived UGTs from the GT-1 family with an emphasis on their acceptor promiscuity and their potential application in glycosylation processes. Recombinant expression in E. coli provided sufficient amounts of enzyme for the in-depth characterization of the salicylic acid UGT from Capsella rubella (UGT-SACr) and the stevia UGT from Stevia rebaudiana (UGT-76G1Sr). The latter was found to have a remarkably broad specificity with activities on a wide diversity of structures, from aliphatic and branched alcohols, over small phenolics to larger flavonoids, terpenoids and even higher glycoside compounds. As an example for its industrial potential, the glycosylation of curcumin was thoroughly evaluated. Under optimized conditions, 96% of curcumin was converted within 24h into the corresponding curcumin β-glycosides. In addition, the reaction was performed in a coupled system with sucrose synthase from Glycine max, to enable the cost-efficient (re)generation of UDP-Glc from sucrose as abundant and renewable resource. Copyright © 2016 Elsevier B.V. All rights reserved.

  12. Directions on the use of stevia leaves (Stevia Rebauidana) as an additive in food products.

    Science.gov (United States)

    Kobus-Moryson, Małgorzata; Gramza-Michałowska, Anna

    2015-01-01

    Due to the high intake of sugars, especially sucrose, global trends in food processing have encouraged producers to use sweeteners, particularly synthetic ones, to a wide extent. For several years, increasing attention has been paid in the literature to the stevia (Stevia rebauidana), containing glycosidic diterpenes, for which sweetening properties have been identified. Chemical composition, nutritional value and application of stevia leaves are briefl y summarized and presented.

  13. EFFECT OF EXPALNT TYPE AND SOME PLANT GROWTH REGULATORS ON CULTURE INITIATION OF STEVIA PLANTS IN VITRO

    National Research Council Canada - National Science Library

    H S M Khierallah; O M A Al-Obaidy

    2017-01-01

    ... المستخلص Stevia rebaudiana Bertoniالستيفيا في نشوء زروعات نبات تأثير نوع الجزء النباتي وبعض منظمات النمو البحث لدراسة اجري إضافة تراكيز تجارب عمى تعقيم واختبار نوعين...

  14. Mechanism of the hypoglycemic effect of stevioside, a glycoside of Stevia rebaudiana.

    Science.gov (United States)

    Chen, Tso-Hsiao; Chen, Shi-Chung; Chan, Paul; Chu, Yen-Ling; Yang, Hung-Yu; Cheng, Juei-Tang

    2005-02-01

    We have studied the effects of stevioside on the glucose and insulin metabolism in 2 models of diabetes in rats, STZ-induced diabetic rats and NIDDM diabetic rats induced by feeding with fructose. Stevioside (0.5 mg/kg), lowered the blood glucose levels in STZ-induced diabetic rats, peaking at 90 min. Stevioside administered twice daily also demonstrated dose-dependent effects in lowering the glucose levels in both diabetic rat models. Stevioside reduced the rise in glucose during glucose tolerance testing in normal rats. Stevioside dose-dependently decreased protein levels of phosphoenol pyruvate carboxykinase (PEPCK) and PEPCK mRNA after 15 days of treatment. Stevioside also reduced insulin resistance in the diabetic animals as shown by the glucose lowering effects of tolbutamide. In conclusion, stevioside was able to regulate blood glucose levels by enhancing not only insulin secretion, but also insulin utilization in insulin-deficient rats; the latter was due to decreased PEPCK gene expression in rat liver by stevioside's action of slowing down gluconeogenesis. Further studies of this agent for the treatment of diabetes appear warranted.

  15. Occurrence of Tomato spotted wilt virus in Stevia rebaudiana and Solanum tuberosum in Northern Greece

    NARCIS (Netherlands)

    Chatzivassiliou, E.K.; Peters, D.; Lolas, P.

    2007-01-01

    Tomato spotted wilt virus (TSWV) (genus Tospovirus, family Bunyaviridae) was first reported in Greece during 1972 (3) and currently is widespread in the central and northern part of the country infecting several cultivated and wild plant species (1,2). In June 2006, virus-like symptoms similar to

  16. Stevia, Nature’s Zero-Calorie Sustainable Sweetener

    Science.gov (United States)

    Ashwell, Margaret

    2015-01-01

    Stevia is a plant native to South America that has been used as a sweetener for hundreds of years. Today, zero-calorie stevia, as high-purity stevia leaf extract, is being used globally to reduce energy and added sugar content in foods and beverages. This article introduces stevia, explaining its sustainable production, metabolism in the body, safety assessment, and use in foods and drinks to assist with energy reduction. The article also summarizes current thinking of the evidence for the role of nonnutritive sweeteners in energy reduction. Overall, stevia shows promise as a new tool to help achieve weight management goals. PMID:27471327

  17. Evaluación de métodos de propagación, fertilización nitrogenada y fenología de estevia en condiciones del Valle del Cauca

    Directory of Open Access Journals (Sweden)

    Carmen R Bonilla C

    2007-09-01

    Full Text Available En el Centro Experimental de la Universidad Nacional de Colombia sede Palmira se evaluó el prendimiento de estacas apicales y basales de Stevia rebaudiana en tres sustratos de enraizamiento (carbonilla-arena, carbonilla-compost y arena-compost y tres fuentes de nitrógeno (urea 46%N, compost 1.5%N y gallinaza 1% N. El mejor método de propagación fue la estaca apical en sustrato carbonilla-arena 1:1, volumen; las fuentes de nitrógeno no presentaron diferencias significativas en la acumulación de materia seca de hojas. Se diferenciaron los siguientes periodos fenológicos: Emergencia: 6 días después de la siembra (dís, Estado vegetativo: 17 dias, Floración: 71 dís, Fructificación: 115 dís, Senescencia: 169 dís y Rebrote: 201 dís

  18. Captación y uso de la radiación en plantas de estevia

    Directory of Open Access Journals (Sweden)

    Jarma O. Alfredo

    2010-04-01

    Full Text Available

    La captación y uso eficiente de la radiación en estevia, especie productora de edulcorantes no calóricos, es un aspecto fundamental de su explotación en regiones cálidas del Neotropico colombiano. Sin embargo, los trabajos sobre estos aspectos son escasos. La presente investigación se realizó en el municipio de Montería (Córdoba, Colombia durante las estaciones secas de 2007 y 2008 (E1 y E2, para determinar el efecto de la radiación fotosintéticamente activa (RFA sobre la producción de biomasa de los genotipos Morita 1 (M1 y Morita 2 (M2, establecidos a densidades de siembra de 25,00; 12,50 y 6,25 plantas/m2. En ambas estaciones se usó un diseño experimental de bloques completos al azar con arreglo factorial 2 x 3. Se utilizó un modelo matemático que cálculo la tasa de acumulación diaria de masa seca (MS en hojas y tallo (simulada con el método de integración de Euler, a partir de la RFA, la fracción de la RFA interceptada por el dosel (FLINT y el uso eficiente de la radiación (UER. En E1 se presentaron en promedio 3 MJ m-2 dia-1 más que en E2, y un desarrollo del índice de área foliar IAF (3,12±0,02 que fue casi tres veces el registrado en E2. Consecuentemente, el valor más alto del coeficiente de extinción de luz a través del dosel (k = 0,21 lo registro M2 durante la estación E1, a la densidad de 25 plantas/m2. Durante este mismo periodo se observaron los valores más altos de UER (2,04 g MJ RFA y el máximo porcentaje de distribución de MS total hacia las hojas, que fue del 60% en M2.

  19. Stevia and stevioside protect against cisplatin nephrotoxicity through inhibition of ERK1/2, STAT3, and NF-κB activation.

    Science.gov (United States)

    Potočnjak, Iva; Broznić, Dalibor; Kindl, Marija; Kropek, Matija; Vladimir-Knežević, Sanda; Domitrović, Robert

    2017-09-01

    We investigated the effect of natural sweetener Stevia rebaudiana and its constituent stevioside in cisplatin (CP)-induced kidney injury. Male BALB/cN mice were orally administered 10, 20, and 50 mg/kg body weight of Stevia rebaudiana ethanol extract (SE) or stevioside 50 mg/kg, 48 h after intraperitoneal administration of CP (13 mg/kg). Two days later, CP treatment resulted in histopathological changes showing kidney injury. Increased expression of 4-hydroxynonenal (4-HNE), 3-nitrotyrosine (3-NT), and heme oxygenase-1 (HO-1) in mice kidneys suggested oxidative stress. CP treatment also increased renal expression of nuclear factor-kappaB (NF-κB) p65 subunit and phosphorylated inhibitor of NF-κB (IκBα), as well as expression of pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α). Induction of apoptosis and inhibition of the cell cycle in kidneys was evidenced by increased expression of p53, Bax, caspase-9, and p21, proteolytic cleavage of poly (ADP-ribose) polymerase (PARP), with concomitant suppression of Bcl-2 and cyclin D1 expression. The number of apoptotic cells in kidneys was also assessed. CP administration resulted in activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) and signal transducer and activator of transcription 3 (STAT3). Both SE and stevioside attenuated CP nephrotoxicity by suppressing oxidative stress, inflammation, and apoptosis through mechanism involving ERK1/2, STAT3, and NF-κB suppression. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Influence of Salicylic Acid on the Antimicrobial Potential of Stevia ...

    African Journals Online (AJOL)

    Purpose: To investigate the effect of salicylic acid (SA) on the antimicrobial profile of Stevia leaf extracts against soybean seed-borne pathogens. Methods: Stevia seeds were planted in a greenhouse and SA foliar applied after six weeks on the whole plant at concentrations of 0 and 0.1 g L-1. The extracts of the plant leaf ...

  1. Потребность во влаге растений стевии Stevia rebaudiana bertoni во время вегетативного развития

    Directory of Open Access Journals (Sweden)

    Инга КУЗНЕЦОВА

    2017-06-01

    Full Text Available The paper presents research results on the water absorption capacity of stevia plants. It was established that, before the first flowering phase, the plants absorb about 2790-2850 ml of moisture. The half of moisture is absorbed by the plants in the first 14 days after seedling planting on ground. At this stage, the thickness of the leaf apparatus increases by 50% of the total thickness. The influence of planting density (45x18 cm; 45x16 cm; 45x12 cm on biometric indices and water absorption capacity of plants was studied. Closer spacing (45x12 cm causes the reduction of plant height to 42 cm and leaf surface area to 4,5 cm3. In addition, the concentrated spacing also causes the increase of the transpiration rates and transpiration coefficient. Therewith, the intensity of moisture evaporation from the surface of leaf apparatus remains unchanged at different planting densities. Increasing planting density from 45x18 cm to 45x12 cm reduces water absorption capacity of plants by 37-40%. Реферат. В работе представлены результаты исследований по способности поглощения влаги растениями стевии. Установлено, что до фазы I цветения растения поглощают 2790-2850 мл влаги. Половину влаги растения поглощают в первые 14 дней после посадки рассады в грунт. В данное время увеличивается толщина листового аппарата на 50% от общей толщины. Исследовано влияние густоты посадки (45x18 см; 45x16 см; 45x12 см на биометрические показатели растений и способность поглощения влаги. Увеличение концентрации посадки по схеме 45х12 см приводит к

  2. Stability of the Stevia-Derived Sweetener Rebaudioside A in Solution as Affected by Ultraviolet Light Exposure.

    Science.gov (United States)

    Zhang, Jiewen; Bell, Leonard N

    2017-04-01

    Rebaudioside A is a natural noncaloric high-potency sweetener extracted from the leaves of Stevia rebaudiana. With rebaudioside A use increasing in foods, understanding the factors affecting its stability is necessary. This project evaluated the degradation rate constants of rebaudioside A in water, 0.1 M phosphate buffer, and 0.1 M citrate buffer at pH 3 and 7 as a function of ultraviolet (UV) light intensity (365 nm, 0 μW/cm 2 for dark conditions, 27 μW/cm 2 for low intensity, and 190 μW/cm 2 for high intensity) at 32.5 °C. Rebaudioside A stability was adversely affected by light exposure. The pseudo-1st-order degradation rate constants increased significantly (P < 0.05) with increasing light intensity in all solutions. Under dark conditions, rebaudioside A in phosphate buffers was more susceptible to breakdown than in water and citrate buffers at both pH levels. However, exposure to UV light resulted in rebaudioside A degradation occurring approximately 10 times faster in citrate than in phosphate buffers at both pH levels. The sensitivity of rebaudioside A to UV light was greater in citrate buffers than in water or phosphate buffers. The use of light-protective packaging for beverages containing rebaudioside A will improve its stability. © 2017 Institute of Food Technologists®.

  3. Isolation and Characterization of a Novel Rebaudioside M Isomer from a Bioconversion Reaction of Rebaudioside A and NMR Comparison Studies of Rebaudioside M Isolated from Stevia rebaudiana Bertoni and Stevia rebaudiana Morita

    Directory of Open Access Journals (Sweden)

    Indra Prakash

    2014-03-01

    Full Text Available A minor product, rebaudioside M2 (2, from the bioconversion reaction of rebaudioside A (4 to rebaudioside D (3, was isolated and the complete structure of the novel steviol glycoside was determined. Rebaudioside M2 (2 is considered an isomer of rebaudioside M (1 and contains a relatively rare 1→6 sugar linkage. It was isolated and characterized with NMR (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D-TOCSY, and NOESY and mass spectral data. Additionally, we emphasize the importance of 1D and 2D NMR techniques when identifying complex steviol glycosides. Numerous NMR spectroscopy studies of rebaudioside M (1, rebaudioside D (3, and mixture of 1 and 3 led to the discovery that SG17 which was previously reported in literature, is a mixture of rebaudioside D (3, rebaudioside M (1, and possibly other related steviol glycosides.

  4. Isolation and Characterization of a Novel Rebaudioside M Isomer from a Bioconversion Reaction of Rebaudioside A and NMR Comparison Studies of Rebaudioside M Isolated from Stevia rebaudiana Bertoni and Stevia rebaudiana Morita

    Science.gov (United States)

    Prakash, Indra; Bunders, Cynthia; Devkota, Krishna P.; Charan, Romila D.; Ramirez, Catherine; Priedemann, Christopher; Markosyan, Avetik

    2014-01-01

    A minor product, rebaudioside M2 (2), from the bioconversion reaction of rebaudioside A (4) to rebaudioside D (3), was isolated and the complete structure of the novel steviol glycoside was determined. Rebaudioside M2 (2) is considered an isomer of rebaudioside M (1) and contains a relatively rare 1→6 sugar linkage. It was isolated and characterized with NMR (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D-TOCSY, and NOESY) and mass spectral data. Additionally, we emphasize the importance of 1D and 2D NMR techniques when identifying complex steviol glycosides. Numerous NMR spectroscopy studies of rebaudioside M (1), rebaudioside D (3), and mixture of 1 and 3 led to the discovery that SG17 which was previously reported in literature, is a mixture of rebaudioside D (3), rebaudioside M (1), and possibly other related steviol glycosides. PMID:24970220

  5. Stevia, Nature's Zero-Calorie Sustainable Sweetener: A New Player in the Fight Against Obesity.

    Science.gov (United States)

    Ashwell, Margaret

    2015-05-01

    Stevia is a plant native to South America that has been used as a sweetener for hundreds of years. Today, zero-calorie stevia, as high-purity stevia leaf extract, is being used globally to reduce energy and added sugar content in foods and beverages. This article introduces stevia, explaining its sustainable production, metabolism in the body, safety assessment, and use in foods and drinks to assist with energy reduction. The article also summarizes current thinking of the evidence for the role of nonnutritive sweeteners in energy reduction. Overall, stevia shows promise as a new tool to help achieve weight management goals.

  6. Overexpression of SrUGT85C2 from Stevia reduced growth and yield of transgenic Arabidopsis by influencing plastidial MEP pathway.

    Science.gov (United States)

    Guleria, Praveen; Masand, Shikha; Yadav, Sudesh Kumar

    2014-04-15

    The transcript expression of a gene SrUGT85C2 has been documented for direct relation with steviol glycoside content in Stevia plant. Steviol glycoside and gibberellin biosynthetic routes are divergent branches of methyl erythritol-4 phosphate (MEP) pathway. So, SrUGT85C2 might be an influencing gibberellin content. Hence in the present study, transgenic Arabidopsis thaliana overexpressing SrUGT85C2 cDNA from Stevia rebaudiana was developed to check its effect on gibberellin accumulation and related plant growth parameters. The developed transgenics showed a noteworthy decrease of 78-83% in GA3 content. Moreover, the transgenics showed a gibberellin deficient phenotype comprising stunted hypocotyl length, reduced shoot growth and a significant fall in relative water content. Transgenics also showed 17-37 and 64-76% reduction in chlorophyll a and chlorophyll b contents, respectively. Reduction in photosynthetic pigments could be responsible for the noticed significant decrease in plant biomass. Like steviol glycoside and gibberellin biosynthesis, chlorophyll biosynthesis also occurs from the precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) of MEP pathway in the plastids. The observed downregulated expression of genes encoding MEP pathway enzymes geranyl geranyl diphosphate synthase (GGDPS), copalyl diphosphate synthase (CDPS), kaurenoic acid oxidase (KAO), chlorophyll synthetase and chlorophyll a oxygenase in transgenics overexpressing SrUGT85C2 might be responsible for the reduction in gibberellins as well as chlorophyll. This study has documented for the first time the regulatory role of SrUGT85C2 in the biosynthesis of steviol glycoside, gibberellins and chlorophyll. Copyright © 2014 Elsevier B.V. All rights reserved.

  7. Rapid Solid-Liquid Dynamic Extraction (RSLDE): a New Rapid and Greener Method for Extracting Two Steviol Glycosides (Stevioside and Rebaudioside A) from Stevia Leaves.

    Science.gov (United States)

    Gallo, Monica; Vitulano, Manuela; Andolfi, Anna; DellaGreca, Marina; Conte, Esterina; Ciaravolo, Martina; Naviglio, Daniele

    2017-06-01

    Stevioside and rebaudioside A are the main diterpene glycosides present in the leaves of the Stevia rebaudiana plant, which is used in the production of foods and low-calorie beverages. The difficulties associated with their extraction and purification are currently a problem for the food processing industries. The objective of this study was to develop an effective and economically viable method to obtain a high-quality product while trying to overcome the disadvantages derived from the conventional transformation processes. For this reason, extractions were carried out using a conventional maceration (CM) and a cyclically pressurized extraction known as rapid solid-liquid dynamic extraction (RSLDE) by the Naviglio extractor (NE). After only 20 min of extraction using the NE, a quantity of rebaudioside A and stevioside equal to 1197.8 and 413.6 mg/L was obtained, respectively, while for the CM, the optimum time was 90 min. From the results, it can be stated that the extraction process by NE and its subsequent purification developed in this study is a simple, economical, environmentally friendly method for producing steviol glycosides. Therefore, this method constitutes a valid alternative to conventional extraction by reducing the extraction time and the consumption of toxic solvents and favouring the use of the extracted metabolites as food additives and/or nutraceuticals. As an added value and of local interest, the experiment was carried out on stevia leaves from the Benevento area (Italy), where a high content of rebaudioside A was observed, which exhibits a sweet taste compared to stevioside, which has a significant bitter aftertaste.

  8. INVITROAND INVIVOEVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT POTENTIAL OF STEVIA EXTRACT.

    Science.gov (United States)

    Moselhy, Said S; Ghoneim, Magdy A; Khan, Jehan A

    2016-01-01

    The current trend globally is the utilization of natural products as therapeutic agents given its minimum side effects. The leaves of Stevia contain several active ingredient compounds such as rebaudioside. Stevia extract have been used for many purposes. Active oxygen radicals can induce base modifications, DNA breakage, and intracellular protein crosslink's. This study was done to evaluate the potential of stevia extract as antibacterial and antioxidants actions. Antibacterial activity of different extracts of stevia was tested in vitro against different species of bacteria and hepato-protective efficacy was testes in rats injected with CCl 4 as hepatotoxic. Acetone extract exhibited antibacterial activity against selected five bacteria species. The acetone extract suppressed the elevation of serum ALT (p stevia extract showed prevention against deleterious effects of CCl 4 by lowering lipid peroxidation and enhancement of antioxidant activities as SOD and CAT. The protection trial is better than treatment trial. Total phenolic content of aqueous and acetone extracts were found 30 mg and 85 mg gallic /gm extract respectively. While the total flavonoids were 40 mg and 80 mg quercetin/g respectively. The GC-MS analysis showed that monoterpene and indole are the main components. Aqueous extract don't show any antibacterial activity against the tested strains. The antioxidant properties were attributable to its phenolic content to scavenge free radicals. Acetone extract possess a potent antimicrobial and activity against deleterious effect of CCl 4 -caused liver damage.

  9. Characteristics of purple nonsulfur bacteria grown under Stevia residue extractions.

    Science.gov (United States)

    Xu, J; Feng, Y; Wang, Y; Lin, X

    2013-11-01

    As a consequence of the large-scale cultivation of Stevia plants, releases of plant residues, the byproduct after sweetener extraction, to the environment are inevitable. Stevia residue and its effluent after batching up contain large amounts of organic matters with small molecular weight, which therefore are a potential pollution source. Meanwhile, they are favourite substrates for micro-organism growths. This investigation was aimed to utilize the simulated effluent of Stevia residue to enrich the representative purple nonsulfur bacterium (PNSB), Rhodopseudomonas palustris (Rps. palustris), which has important economic values. The growth profile and quality of Rps. palustris were characterized by spectrophotometry, compared to those grown in common PNSB mineral synthetic medium. Our results revealed that the simulated effluent of Stevia residue not only stimulated Rps. palustris growth to a greater extent, but also increased its physiologically active cytochrome concentrations and excreted indole-3-acetic acid (IAA) content. This variation in phenotype of Rps. palustris could result from the shift in its genotype, further revealed by the repetitive sequence-based PCR (rep-PCR) fingerprinting analysis. Our results showed that the effluent of Stevia residue was a promising substrate for microbial growth. © 2013 The Society for Applied Microbiology.

  10. Chitosan as flocculant agent for clarification of stevia extract

    Directory of Open Access Journals (Sweden)

    Silvia P. D. de Oliveira

    2012-01-01

    Full Text Available Stevia is used as a sweetener due to its low calorific value and its taste, which is very similar to that of sucrose. After extraction from dried leaves, stevia extract is dark in colour, and therefore needs to be whitened to increase acceptance by consumers. In this study we tested chitosan, a cationic polyelectrolyte, as flocculant agent for the whitening of the Stevia extract. Positive charges of chitosan can interact electrostatically with a counter-ion, sodium tripolyphosphate (TPP, and then chitosan precipitates. A factorial design was used to study the whitening process, in which Glycosides Removal, Colour Removal, Turbidity Removal and Soluble Solids Removal were evaluated. The studied factors were Chitosan Mass and pH of the TPP solution. The results showed that chitosan is a good flocculant agent, being able to flocculate both the glycosides and the pigments that make the extract coloured.

  11. PEMBUATAN GULA NON KARSINOGENIK NON KALORI DARI DAUN STEVIA

    Directory of Open Access Journals (Sweden)

    Luqman Buchori

    2011-07-01

    Full Text Available Stevia merupakan bahan pemanis selain tebu dengan kelebihan tingkat kemanisan 200 – 300 kali dari gula tebu dan diperoleh dari estrak daun stevia. Pembuatan gula stevia dilakukan dengan metode ekstraksi untuk mengambil stevioside kemudian dipekatkan dengan cara evaporasi lalu dikristalisasi sehingga diperoleh kristal stevioside. Dalam penelitian ini dicari pengaruh suhu (45°C; 50°C; 55°C, pengaruh waktu ekstraksi (1 ; 1,5 ; 2 jam, serta pengaruh jenis solvent yang digunakan (metanol, etanol, aseton terhadap berat gula yang didapatkan. Dari hasil penelitian diperoleh bahwa semakin tinggi suhu maka jumlah produk yang terekstrak semakin banyak, baik untuk solvent metanol maupun etanol. Sedangkan untuk solvent aseton kondisi optimum dicapai pada suhu 50°C. Untuk waktu ekstraksi, semakin lama waktu ekstraksi jumlah produk yang terekstrak semakin banyak. Untuk jenis solvent yang digunakan, metanol lebih banyak mengekstrak produk dibanding etanol maupun aseton. Produk yang diperoleh mempunyai range pH antara 5,2 – 5,5, titik leleh antara 196 – 198 °C, dan densitas antara 1,43 – 1,67, dimana harga-harga tersebut berada di dalam range pH, titik leleh, dan densitas gula stevia.

  12. Composition of antioxidants and amino acids in Stevia leaf infusions.

    Science.gov (United States)

    Periche, Angela; Koutsidis, Georgios; Escriche, Isabel

    2014-03-01

    Stevia, a non-caloric natural sweetener with beneficial properties and considerable antioxidants and amino acids, is increasingly consumed as an infusion. This work evaluates the influence of the conditions (temperature: 50, 70 or 90 °C and time: 1, 5, 20 or 40 min) applied to obtain Stevia infusions, on antioxidants (total phenols, flavonoids and antioxidant activity) and amino acids. The total concentration of the eleven amino acids found was 11.70 mg/g in dried leaves and from 6.84 to 9.11 mg/g per gram of Stevia in infusions. However, infusions showed higher levels of certain amino acids (alanine, asparagine, leucine and proline), and greater values of the three antioxidant parameters in comparison with dry leaves. Temperature had more influence (minimum values at 50 °C and maximum at 90 °C) than time in the case of antioxidants. At 90 °C there were no important increases in the extraction of antioxidant compounds after 5 min; each gram of Stevia had 117 mg trolox (total antioxidant activity), 90 mg gallic acid (total phenols) and 56 mg catechin equivalents (flavonoids). Varying the temperature and time conditions no notable differences were observed in the concentrations of the majority of amino acids. However, the infusion treatment at 90 °C for 5 min was the best, as it gave the highest yield of 8 of the 11 amino acids. Therefore, with respect to the compounds analyzed in this study, the best way to obtain Stevia leaf infusions is the same as the domestic process, almost boiling water for a short time.

  13. [ASSOCIATION BETWEEN STEVIA SWEETENER CONSUMPTION AND NUTRITIONAL STATUS IN UNIVERSITY STUDENTS].

    Science.gov (United States)

    Durán Agüero, Samuel; Vásquez Leiva, Alejandra; Morales Illanes, Gladys; Schifferli Castro, Ingrid; Sanhueza Espinoza, Claudia; Encina Vega, Claudia; Vivanco Cuevas, Karla; Mena Bolvaran, Rodrigo

    2015-07-01

    stevia consumption has increased worlwide among the different age groups; however, studies regarding the association between stevia intake and nutritional status in adults are scarce. to evaluate stevia intake in first year university students from five chilean cities (Santiago, Temuco, Viña del Mar, Concepción and Antofagasta) controlling by nutritional status, socioeconomic level, gender and whether their undergraduate program belongs to the health sciences. 486 first year university students belonging to 4 Chilean universities were evaluated. Each student completed a weekly food frequency questionnaire including food and beverages containing stevia. Selfreport of weight and height was requested. 69.8% of the students consumed stevia every week, the liquid form being the main contributor to the dietary stevia intake (81.2%). Only 1.4% of the students went over the Acceptable Daily Intake (ADI). Normal weight women show a higher stevia intake compared to those obese or overweight (p stevia consumption appears to be positively associated to normal weight in the first model (adjusted) (OR = 0.219; IC 95%: 0.13-0.35; p stevia consumption was positively associated with normal nutritional status in Chilean university students. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.

  14. Nanocomposite formation between alpha-glucosyl stevia and surfactant improves the dissolution profile of poorly water-soluble drug.

    Science.gov (United States)

    Uchiyama, Hiromasa; Tozuka, Yuichi; Nishikawa, Masahiro; Takeuchi, Hirofumi

    2012-05-30

    The formation of a hybrid-nanocomposite using α-glucosyl stevia (Stevia-G) and surfactant was explored to improve the dissolution of flurbiprofen (FP). As reported previously, the dissolution amount of FP was enhanced in the presence of Stevia-G, induced by the formation of an FP and Stevia-G-associated nanostructure. When a small amount of sodium dodecyl sulfate (SDS) was present with Stevia-G, the amount of dissolved FP was extremely enhanced. This dissolution-enhancement effect was also observed with the cationic surfactant of dodecyl trimethyl ammonium bromide, but not with the non-ionic surfactant of n-octyl-β-D-maltopyranoside. To investigate the dissolution-enhancement effect of Stevia-G/SDS mixture, the pyrene I(1)/I(3) ratio was plotted versus the Stevia-G concentration. The pyrene I(1)/I(3) ratio of Stevia-G/SDS mixture had a sigmoidal curve at lower Stevia-G concentrations compared to the Stevia-G solution alone. These results indicate that the Stevia-G/SDS mixture provides a hydrophobic core around pyrene molecules at lower Stevia-G concentrations, leading to nanocomposite formation between Stevia-G and SDS. The nanocomposite of Stevia-G/SDS showed no cytotoxicity to Caco-2 cells at a mixture of 0.1% SDS and 1% Stevia-G solution, whereas 0.1% SDS solution showed high toxicity. These results suggest that the nanocomposite formation of Stevia-G/SDS may be useful way to enhance the dissolution of poorly water-soluble drugs without special treatment. Copyright © 2012 Elsevier B.V. All rights reserved.

  15. Effect of Inulin and Stevia on Some Physical Properties of Chocolate Milk

    Science.gov (United States)

    Homayouni Rad, Aziz; Delshadian, Zohre; Arefhosseini, Seyed Rafi; Alipour, Beitollah; Asghari Jafarabadi, Mohammad

    2012-01-01

    Background: The aim of this study was to assess physical properties of dietetic chocolate milk produced by stevia as a sugar replacer and inulin. Along with having prebiotic effect, inulin can also participate in enhancing textural properties of beverages. Therefore, this novel food will be useful for all people especially for diabetics. Methods: This study was carried out in Quality Control Laboratory of Food Science and Technology Department, Health and Nutrition Faculty, Tabriz University of Medical Science, during 2011-2012. The assay was performed on nine treatments with three replications. Sugar was substituted with stevia in two proportions of sucrose to stevia; 50:50 and 0:100. Inulin, in four levels of 0%, 2%, 4% and 6%, was added to the treatments of 50% and 100% stevia. The control sample contained no stevia and no inulin, such as commercial ones. Precipitation amount and viscosity were measured 24 hours after production. Data analyzed by one-way ANOVA, at the significant level of 0.05, using SPSS software ver. 17. Results: Sugar replacement with stevia caused significant increase in precipitation and significant decrease in viscosity (Pstevia and 6% inulin revealed a viscosity with a non-significant difference with the control (P>0.05). The sample containing 50% stevia and 6% inulin had the lowest precipitation while having a non-significantly difference with the control. Conclusion A thickener agent, such as inulin, should be used in the chocolate milk sweetened by stevia to improve physical properties of the product. PMID:24688916

  16. Effect of Inulin and Stevia on Some Physical Properties of Chocolate Milk

    Directory of Open Access Journals (Sweden)

    Mohammad Asghari Jafarabadi

    2012-07-01

    Full Text Available Background: The aim of this study was to assess physical properties of dietetic chocolate milkproduced by stevia as a sugar replacer and inulin. Along with having prebiotic effect, inulin canalso participate in enhancing textural properties of beverages. Therefore, this novel food will beuseful for all people especially for diabetics.Methods: This study was carried out in Quality Control Laboratory of Food Science and TechnologyDepartment, Health and Nutrition Faculty, Tabriz University of Medical Science, during2011-2012. The assay was performed on nine treatments with three replications. Sugar was substitutedwith stevia in two proportions of sucrose to stevia; 50:50 and 0:100. Inulin, in four levelsof 0%, 2%, 4% and 6%, was added to the treatments of 50% and 100% stevia. The control samplecontained no stevia and no inulin, such as commercial ones. Precipitation amount and viscositywere measured 24 hours after production. Data analyzed by one-way ANOVA, at the significantlevel of 0.05, using SPSS software ver. 17.Results: Sugar replacement with stevia caused significant increase in precipitation and significantdecrease in viscosity (P0.05. The sample containing50% stevia and 6% inulin had the lowest precipitation while having a non-significantlydifference with the control.Conclusion: A thickener agent, such as inulin, should be used in the chocolate milk sweetenedby stevia to improve physical properties of the product.

  17. Preference of stevia level in Coca Cola: Preliminary findings in 50+ year olds

    DEFF Research Database (Denmark)

    Panovská, Zdenka; Grosová, Stanislava; Sudzina, Frantisek

    2017-01-01

    Coca Cola Life was first introduced in Argentina in June 2013. In the following 15 months, it was launched also in Chile, Sweden, and the U.K. Since January 2015, it is available in many but not all countries. In the version of Coca Cola Life, which was produced till December 2016, 35% of sugar...... was replaced by stevia. In the version produced since January 2017, 45% of sugar is replaced by stevia. This opened a limited time-frame for studying preferences between regular Coca Cola, Coca Cola Life with 35% of stevia, and Coca Cola Life with 45% of stevia. The aim of the paper is to investigate if Big...... Five Inventory personality traits, gender, age, smoking, and drinking of any cola in general influence preferred amount of stevia. The research was conducted in the Czech Republic where Coca Cola Life is not available, so all respondents are equally unaware of (or equally not used to) the taste. All...

  18. The antimicrobial potential of stevia in an in vitro microbial caries model.

    Science.gov (United States)

    Kishta-; Derani, Maryam; Neiva, Gisele F; Boynton, James R; Kim, Youngjoo E; Fontana, Margherita

    2016-04-01

    To determine the effect of stevia on caries development when incorporated into a cariogenic diet in a controlled microbial caries model. 56 bovine tooth specimens (4 x 4 mm) were divided into four groups, each secured in a caries-forming vessel. All vessels were placed on an electric stirrer inside a 37°C incubator. The specimens were inoculated with Streptococcus mutans, and exposed for 4 days to circulating cycles of tryptic soy broth supplemented with 5% sucrose-TSBS (three x/day), and a mineral wash solution. Between TSBS cycles (three x/day), each group received one of four experimental solutions: phosphate buffer (PBS-negative control), 0.5% stevia solution, 5% stevia solution, or 5% xylitol solution. Development of caries lesions was analyzed using enamel surface hardness. Difference in Vickers Hardness between pre and post-treatment was calculated to determine caries development. Plaque was dislodged from six specimens per group, and the CFU/ml calculated. Data were analyzed using ANOVA at 95% confidence level, and individual group differences calculated using Tukey's test. 5% xylitol resulted in significantly less plaque at the end of the study compared to PBS and 5% stevia, but not significantly different than 0.5% stevia. 5% stevia had significantly softer lesions than the other groups, while there was no significant difference in hardness scores between 5% xylitol, 0.5% stevia and PBS.

  19. Transglycosylated stevia and hesperidin as pharmaceutical excipients: dramatic improvement in drug dissolution and bioavailability.

    Science.gov (United States)

    Uchiyama, Hiromasa; Tozuka, Yuichi; Imono, Masaaki; Takeuchi, Hirofumi

    2010-10-01

    The capability of transglycosylated materials, α-glycosyltransferase-treated stevia (Stevia-G) and α-glycosyl hesperidin (Hsp-G), to enhance the bioavailability of poorly water-soluble drugs was investigated. Spray-dried particles (SDPs) of drug/transglycosylated material, such as, flurbiprofen (FP)/Stevia-G, probucol (PRO)/Stevia-G, FP/Hsp-G, and PRO/Hsp-G were prepared. All SDPs showed pronounced improvement in both dissolution rate and apparent drug solubility. The amount of dissolved PRO was significantly improved to that of untreated PRO crystals when prepared as SDPs of PRO/Stevia-G or PRO/Hsp-G. There was no cytotoxicity to Caco-2 cells at levels of 10% Stevia-G or Hsp-G solution. Values for the area under the plasma concentration-time curve (AUC) of untreated PRO, SDPs of PRO/Hsp-G and PRO/Stevia-G after oral administration to rats were 4.94±2.06, 26.08±4.52 and 48.79±9.97μgh/mL, respectively. Interestingly, AUC values in cases of the FP system were in the order of untreated FPStevia-GStevia-G, a newly investigated material for this purpose, was found to have good potential for use as a pharmaceutical excipient. Copyright © 2010 Elsevier B.V. All rights reserved.

  20. Extraction of stevia glycosides with CO2 + water, CO2 + ethanol, and CO2 + water + ethanol

    Directory of Open Access Journals (Sweden)

    A. Pasquel

    2000-09-01

    Full Text Available Stevia leaves are an important source of natural sugar substitute. There are some restrictions on the use of stevia extract because of its distinctive aftertaste. Some authors attribute this to soluble material other than the stevia glycosides, even though it is well known that stevia glycosides have to some extent a bitter taste. Therefore, the purpose of this work was to develop a process to obtain stevia extract of a better quality. The proposed process includes two steps: i Pretreatment of the leaves by SCFE; ii Extraction of the stevia glycosides by SCFE using CO2 as solvent and water and/or ethanol as cosolvent. The mean total yield for SCFE pretreatment was 3.0%. The yields for SCFE with cosolvent of stevia glycosides were below 0.50%, except at 120 bar, 16°C, and 9.5% (molar of water. Under this condition, total yield was 3.4%. The quality of the glycosidic fraction with respect to its capacity as sweetener was better for the SCFE extract as compared to extract obtained by the conventional process. The overall extraction curves were well described by the Lack extended model.

  1. In vitro bioassay investigations of the endocrine disrupting potential of steviol glycosides and their metabolite steviol, components of the natural sweetener Stevia.

    Science.gov (United States)

    Shannon, Maeve; Rehfeld, Anders; Frizzell, Caroline; Livingstone, Christina; McGonagle, Caoimhe; Skakkebaek, Niels E; Wielogórska, Ewa; Connolly, Lisa

    2016-05-15

    The food industry is moving towards the use of natural sweeteners such as those produced by Stevia rebaudiana due to the number of health and safety concerns surrounding artificial sweeteners. Despite the fact that these sweeteners are natural; they cannot be assumed safe. Steviol glycosides have a steroidal structure and therefore may have the potential to act as an endocrine disruptor in the body. Reporter gene assays (RGAs), H295R steroidogenesis assay and Ca(2+) fluorimetry based assays using human sperm cells have been used to assess the endocrine disrupting potential of two steviol glycosides: stevioside and rebaudioside A, and their metabolite steviol. A decrease in transcriptional activity of the progestagen receptor was seen following treatment with 25,000 ng/ml steviol in the presence of progesterone (157 ng/ml) resulting in a 31% decrease in progestagen response (p=<0.01). At the level of steroidogenesis, the metabolite steviol (500-25,000 ng/ml) increased progesterone production significantly by 2.3 fold when exposed to 10,000 ng/ml (p=<0.05) and 5 fold when exposed to 25,000 ng/ml (p=<0.001). Additionally, steviol was found to induce an agonistic response on CatSper, a progesterone receptor of sperm, causing a rapid influx of Ca(2+). The response was fully inhibited using a specific CatSper inhibitor. These findings highlight the potential for steviol to act as a potential endocrine disruptor. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  2. Mixed Micelle System Produced by Interaction Between Transglycosylated Stevia and an Ionic Surfactant Improves Dissolution Profile of Mefenamic Acid.

    Science.gov (United States)

    Fujimori, Miki; Kadota, Kazunori; Tozuka, Yuichi

    2017-04-01

    Transglycosylated stevia (stevia-G) can effectively improve the dissolution and bioavailability of poorly water-soluble drugs. Furthermore, addition of an ionic surfactant to stevia-G solution has been shown to enhance the dissolution effect of stevia-G on flurbiprofen. Herein, 4 surfactants, namely sodium dodecyl sulfate, sodium N-dodecanoylsarcosinate, sodium monododecyl phosphate, and lauryltrimethylammonium chloride (LTAC) were screened to investigate their synergistic effect with stevia-G in enhancing the solubility of mefenamic acid (MFA). The ternary formulation containing LTAC produced the highest increase in solubility, whereas the binary MFA/LTAC formulation did not increase the solubility of MFA. Surface tension was evaluated to analyze the interaction between stevia-G and each ionic surfactant, wherein the Rubingh model was applied to predict mixed micelle formation between stevia-G and LTAC. Interaction parameters calculated by the Rubingh model reflected mixed micelle formation between stevia-G and LTAC relative to the self-interactions of the 2 individual surfactants. All interaction parameters in this system showed negative values, indicating a favorable interaction (e.g., hydrogen bond or electrostatic and dipole) between binary components in the mixed micelles. Spray-dried particles of ternary formulations (MFA/stevia-G/LTAC) were prepared to evaluate the dissolution profile and physicochemical properties. Dissolution profiling showed that the concentration of MFA released from spray-dried particles was significantly higher than untreated MFA. Copyright © 2017 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.

  3. Classification of stevia sweeteners in soft drinks using liquid chromatography and time-of-flight mass spectrometry.

    Science.gov (United States)

    Kakigi, Y; Suzuki, T; Icho, T; Uyama, A; Mochizuki, N

    2013-01-01

    The aim of this study was to develop a comprehensive analytical method for the characterisation of stevia sweeteners in soft drinks. By using LC and time-of-flight MS, we detected 30 steviol glycosides from nine stevia sweeteners. The mass spectral data of these compounds were applied to the analysis to determine steviol glycosides in nine soft drinks. On the basis of chromatographic data and principal-component analysis, these soft drinks were classified into three groups, and the soft drinks of each group, respectively, contained high-rebaudioside A extract, normal stevia extract or alfa-glucosyltransferase-treated stevia extract.

  4. Effects of stevia, aspartame, and sucrose on food intake, satiety, and postprandial glucose and insulin levels

    Science.gov (United States)

    Anton, Stephen D.; Martin, Corby K.; Han, Hongmei; Coulon, Sandra; Cefalu, William T.; Geiselman, Paula; Williamson, Donald A.

    2010-01-01

    Consumption of sugar-sweetened beverages may be one of the dietary causes of metabolic disorders, such as obesity. Therefore, substituting sugar with low-calorie sweeteners may be an efficacious weight management strategy. We tested the effect of preloads containing stevia, aspartame, or sucrose on food intake, satiety, and postprandial glucose and insulin levels. Design: 19 healthy lean (BMI = 20.0 – 24.9) and 12 obese (BMI = 30.0 – 39.9) individuals 18 to 50 years old completed three separate food test days during which they received preloads containing stevia (290 kcal), aspartame (290 kcal), or sucrose (493 kcal) before the lunch and dinner meal. The preload order was balanced, and food intake (kcal) was directly calculated. Hunger and satiety levels were reported before and after meals, and every hour throughout the afternoon. Participants provided blood samples immediately before and 20 minutes after the lunch preload. Despite the caloric difference in preloads (290 vs. 493 kcals), participants did not compensate by eating more at their lunch and dinner meals when they consumed stevia and aspartame versus sucrose in preloads (mean differences in food intake over entire day between sucrose and stevia = 301 kcal, p Stevia preloads significantly lowered postprandial glucose levels compared to sucrose preloads (p stevia and aspartame preloads, participants did not compensate by eating more at either their lunch or dinner meal and reported similar levels of satiety compared to when they consumed the higher calorie sucrose preload. PMID:20303371

  5. La Stevia rebaudiana como coadyuvante en la prevención y el control de la caries dental: una revisión de literatura

    OpenAIRE

    Andrea Elena Paredes Vélez; María Claudia Naranjo Sierra

    2016-01-01

    Introducción: La caries dental es la enfermedad crónica más prevalente en el mundo, y en Colombia, como en otros países, es considerada como un problema de salud pública. Es una enfermedad compleja, dinámica, y para su desarrollo intervienen muchos factores; la dieta rica en carbohidratos, es uno de los más significativos. La sacarosa se ha relacionado con problemas de salud como la caries, por ello, es deseable su reemplazo por edulcorantes con menos efectos adversos, y que aporten beneficio...

  6. Determination of eight artificial sweeteners and common Stevia rebaudiana glycosides in non-alcoholic and alcoholic beverages by reversed-phase liquid chromatography coupled with tandem mass spectrometry.

    Science.gov (United States)

    Kubica, Paweł; Namieśnik, Jacek; Wasik, Andrzej

    2015-02-01

    The method for the determination of acesulfame-K, saccharine, cyclamate, aspartame, sucralose, alitame, neohesperidin dihydrochalcone, neotame and five common steviol glycosides (rebaudioside A, rebaudioside C, steviol, steviolbioside and stevioside) in soft and alcoholic beverages was developed using high-performance liquid chromatography and tandem mass spectrometry with electrospray ionisation (HPLC-ESI-MS/MS). To the best of our knowledge, this is the first work that presents an HPLC-ESI-MS/MS method which allows for the simultaneous determination of all EU-authorised high-potency sweeteners (thaumatin being the only exception) in one analytical run. The minimalistic sample preparation procedure consisted of only two operations; dilution and centrifugation. Linearity, limits of detection and quantitation, repeatability, and trueness of the method were evaluated. The obtained recoveries at three tested concentration levels varied from 97.0 to 105.7%, with relative standard deviations lower than 4.1%. The proposed method was successfully applied for the determination of sweeteners in 24 samples of different soft and alcoholic drinks.

  7. ABSORÇÃO E ACUMULAÇÃO DE NUTRIENTES EM ESTÉVIA Stevia rebaudiana (Bert.) Bertoni: II. MICRONUTRIENTES

    OpenAIRE

    Lima Filho O.F. de; Malavolta E; Sena .O.A. de; Carneiro J.W.P

    1997-01-01

    Estudou-se a absorção e o acúmulo de micronutrientes pela estévia, em condições de cultivo comercial no campo. Foram avaliados dois índices nutricionais: Taxa de Absorção Relativa do Nutriente e Taxa de Acumulação Líquida Organogênica do Nutriente. Foram realizadas 7 coletas a cada 15 dias a partir do transplante. Pouco antes ou no início do florescimento, a produção de 1 tonelada de folhas secas exigiu, em g: B-89; Cu-26; Fe-638; Mn-207; Zn-13. Para a produção de sementes, correspondente a 1...

  8. ABSORÇÃO E ACUMULAÇÃO DE NUTRIENTES EM ESTÉVIA Stevia rebaudiana (Bert. Bertoni: II. MICRONUTRIENTES

    Directory of Open Access Journals (Sweden)

    Lima Filho O.F. de

    1997-01-01

    Full Text Available Estudou-se a absorção e o acúmulo de micronutrientes pela estévia, em condições de cultivo comercial no campo. Foram avaliados dois índices nutricionais: Taxa de Absorção Relativa do Nutriente e Taxa de Acumulação Líquida Organogênica do Nutriente. Foram realizadas 7 coletas a cada 15 dias a partir do transplante. Pouco antes ou no início do florescimento, a produção de 1 tonelada de folhas secas exigiu, em g: B-89; Cu-26; Fe-638; Mn-207; Zn-13. Para a produção de sementes, correspondente a 1 tonelada de folhas secas, a extração de micronutrientes, em g, foi: B-226; Cu-76; Fe-2550; Mn-457; Zn-33.

  9. ABSORÇÃO E ACUMULAÇÃO DE NUTRIENTES EM ESTÉVIA Stevia rebaudiana (Bert. Bertoni: I. MACRONUTRIENTES

    Directory of Open Access Journals (Sweden)

    Lima Filho O.F. de

    1997-01-01

    Full Text Available Estudou-se a absorção e o acúmulo de macronutrientes pela estévia, em condições de cultivo comercial no campo. Foram avaliados dois índices nutricionais: Taxa de Absorção Relativa do Nutriente e Taxa de Acumulação Líquida Organogênica do Nutriente. Foram realizadas 7 coletas a cada 15 dias a partir do transplante. Pouco antes ou no início do florescimento, a produção de 1 tonelada de folhas secas exigiu, em kg: N-64,6; P-7,6; K-56,1; Ca-15,8; Mg-3,6 e S-3,6. Para a produção de sementes, correspondente a 1 tonelada de folhas secas, a extração de macronutrientes, em kg, foi: N-130; P-18,8; K-131,5; Ca-43,7; Mg-8,3 e S-9,7.

  10. Extraction of steviol glycosides from fresh Stevia using acidified water; comparison to hot water extraction, including purification

    NARCIS (Netherlands)

    Kootstra, A.M.J.; Huurman, Sander

    2017-01-01

    This report describes a practical comparison of an acidified water extraction of freshly harvested Stevia
    plants (the NewFoss method) to the hot water extraction of dried Stevia plants, the industry standard. Both
    extracts are subsequently purified using lab-/bench scale standard industrial

  11. The influence of stevia glycosides on the growth of Lactobacillus reuteri strains.

    Science.gov (United States)

    Deniņa, I; Semjonovs, P; Fomina, A; Treimane, R; Linde, R

    2014-03-01

    Use of stevia-derived sweeteners was recently officially approved by the European Commission, and their application in the food industry has increased, especially in functional foods. However, there are scarce data about the influence of stevia on probiotic bacteria, which are important both as an inhabitant of the human gut and as a functional food additive. Taking into consideration the broad application of Lactobacillus reuteri in functional foods, the aim of the research was to evaluate the influence of stevia glycosides on its growth. Six Lact. reuteri strains were tested for their ability to grow in the presence of stevioside and rebaudioside A (0·2-2·6 g l(-1) ). The effect of stevia glycosides on biomass concentration, cell count, pH and lactic and acetic acid synthesis was analysed. Both glycosides impaired the growth of analysed strains. However, the inhibitory effect was strain specific, and the concentration-dependent effect was not observed for all parameters. The most pronounced concentration-dependent effect was on lactic and acetic acid production. Taking into account the observed strain-specific inhibitory effect of stevia glycosides, it could be suggested to evaluate the influence of them on each strain employed before their simultaneous application in functional foods. The study showed that the growth of Lactobacillus reuteri strains was inhibited in the presence of stevia sweeteners stevioside and rebaudioside A. Probiotics, for example Lact. reuteri strains, are often used as functional additives in health foods and are an important natural inhabitant of the human gastrointestinal tract. Stevia glycosides application in food is increasing; yet, there are no data about the influence of stevia glycosides on Lact. reuteri growth and very few data on growth of other lactobacilli, either in probiotic foods or in the gastrointestinal tract. This research shows that it is necessary to evaluate the influence of stevia glycosides on other groups

  12. Use of Coffee Silverskin and Stevia to Improve the Formulation of Biscuits

    Directory of Open Access Journals (Sweden)

    Garcia-Serna Esther

    2014-12-01

    Full Text Available Decreasing the amount of sugar added to biscuits is a good strategy to obtain a healthy product. However, a reduction in the quantity of sugar may affect its nutritional value and quality. The feasibility of the combined use of stevia and coffee silverskin for achieving healthier, nutritious and good quality biscuits has been investigated. Ten wheat four biscuit formulations were designed. Sucrose, maltitol and stevia were used as sweeteners and coffee silverskin was used as a natural colouring and as a source of dietary fbre. The quality of the biscuits was evaluated by measuring their moisture, thickness, breaking force and colour. Acrylamide (ACR and hydroxymethylfurfural (HMF contents were also determined in the interest of food safety. The quality and safety of the innovative biscuits was obtained by an analysis of the sugars, proteins, free amino acids, chlorogenic acid, overall antioxidant capacity and acrylamide after in vitro digestion. Only the stevia biscuits and those added with coffee silverskin extract and the solid residue recovered from the extraction process, were selected for that study. A comparison of the stevia formulated biscuits, with the stevia formula added with silverskin, showed that the added biscuits had a good nutritional quality and improved texture and colour.

  13. Production and physicochemical assessment of new stevia amino acid sweeteners from the natural stevioside.

    Science.gov (United States)

    Khattab, Sherine N; Massoud, Mona I; Jad, Yahya El-Sayed; Bekhit, Adnan A; El-Faham, Ayman

    2015-04-15

    New stevia amino acid sweeteners, stevia glycine ethyl ester (ST-GL) and stevia l-alanine methyl ester (ST-GL), were synthesised and characterised by IR, NMR ((1)H NMR and (13)C NMR) and elemental analysis. The purity of the new sweeteners was determined by HPLC and their sensory properties were evaluated relative to sucrose in an aqueous system. Furthermore, the stevia derivatives (ST-GL and ST-AL) were evaluated for their acute toxicity, melting point, solubility and heat stability. The novel sweeteners were stable in acidic, neutral or basic aqueous solutions maintained at 100 °C for 2 h. The sweetness intensity rate of the novel sweeteners was higher than sucrose. Stevia amino acid (ST-GL and ST-AL) solutions had a clean sweetness taste without bitterness when compared to stevioside. The novel sweeteners can be utilised as non-caloric sweeteners in the production of low-calorie food. Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. Stevia, cyclamate and saccharin - natural and artificial sweeteners - exert no effect on sulfane levels in tissues.

    Science.gov (United States)

    Wilinski, Bogdan; Opoka, Wlodzimierz; Somogyi, Eugeniusz; Piotrowska, Joanna; Wilinski, Jerzy

    2013-01-01

    The interactions among natural and artificial sweeteners and endogenous sulfur metabolism have never been investigated. CBA strain mice were administered orally stevia, cyclamate or saccharin in doses of 5 mg/kg of body weight in water solutions each. The measurements of the free and acid-labile sulfane (H2S) tissue concentrations in brain, heart, liver and kidney were performed with Siegel spectrophotometric modified method. No differences in comparisons between hydrogen sulfide concentrations in the control group and each sweetener group within every tissue type were noted. In conclusion, stevia, cyclamate and saccharine do not change the endogenous sulfur metabolism to the extent of causing sulfane tissue levels alterations.

  15. In vitro conservation of twenty-three overexploited medicinal plants belonging to the Indian sub continent

    National Research Council Canada - National Science Library

    Verma, Priyanka; Mathur, Ajay Kumar; Jain, Sheetal Prasad; Mathur, Archana

    2012-01-01

    ..., Coleus forskohlii, Gentiana kurroo, Saussurea lappa, Stevia rebaudiana, Acorus calamus, Pyrethrum cinerariaefolium, Aloe vera, Bacopa monnieri, Salvia sclarea, Glycyrrhiza glabra, Swertia cordata...

  16. Extraction of steviol glycosides from fresh Stevia using acidified water; clarification followed by ultrafiltration and nanofiltration

    NARCIS (Netherlands)

    Kootstra, A.M.J.; Elissen, H.J.H.; Huurman, Sander

    2016-01-01

    As part of the PPS Kleinschalige bioraffinage project (WP1b), fresh Stevia material was used in the extraction of steviol glycosides using water acidified through conversion of sugar by microorganisms naturally present on the plant. Two successive harvests from the same plot were used. Previous

  17. KEAMANAN STEVIA HASIL BUDIDAYA B2P2TO2T DALAM ASPEK TERATOGENITAS

    Directory of Open Access Journals (Sweden)

    Lucie Widowati

    2012-07-01

    Full Text Available Teratogenic test has been performed on sweet stevia as product of B2P2TO2T cultivation at  rats (Rattus novergicus pregnant female  Wistar. Stevia sweet was administered orally at a dose of 360, 120 and 40 mg/kg bw, volume 1 ml/100g bw per day during organogenesis period, on the day of pregnancy to the 6th until 15th. During the test, test animals were observed two times daily with the distance of six hours against the toxicity symptoms such as changes in skin, hair, eyes and mucous membranes, bleeding. Animals that experienced abortion, premature birth or death during the trial period were sacrificed and observed immediately with microscopic technic. At 20th day of pregnancy all of the pregnant rat dissected, and put out the fetuses  from the mother's and observated the health conditions in general and  the whole of mothers reproductive systems of fetuses, the outer fetal malformation and soft tissue system of fetal. The conclusion were  sweet stevia  of B2P2TO2T  no worse effect on the mother rats, fetal body weight and morphology of mother rats and the fetus, does not affect the process of development of fetal soft tissue, and does not affect the development of fetal skeleton. Generally the B2P2TO2T stevia sweet substances safe for used, does not cause teratogenic effects.

  18. Effect of Consuming Tea with Stevia on Salivary pH - An In Vivo Randomised Controlled Trial.

    Science.gov (United States)

    Pallepati, Akhil; Yavagal, Puja; Veeresh, D J

    To assess the effect of consuming tea with stevia on salivary pH. This randomised controlled trial employed a Latin square design. Twenty-four male students aged 20-23 years were randomly allocated to 4 different groups, 3 experimental with tea sweetened by sucrose, jaggery or stevia, and one unsweetened control. Salivary pH assessments were performed at baseline and 1 min, 20 and 60 min after consumption of the respective tea. One-way ANOVA and repeated measures ANOVA followed by Tukey's post-hoc tests were employed to analyse the data. One minute after tea consumption, the salivary pH of the sucrose group significantly decreased compared to the stevia group (p = 0.01). There was a significant difference between baseline mean salivary pH and post-interventional mean salivary pH values at all time intervals in the tea + sucrose, tea + jaggery, and plain tea groups (p stevia and plain tea groups, but it remained lower in the sucrose and jaggery groups. The results of the present study, in which the salivary pH values returned to baseline pH 1 h after drinking stevia-sweetened tea, suggest stevia's potential as a non-cariogenic sweetener.

  19. The effect of herbal formula PROVE 1 and Stevia levels in diets on diet utilization of growing pigs

    Directory of Open Access Journals (Sweden)

    Kooprasert, S.

    2007-05-01

    Full Text Available The objective of this experiment was to study the effect of 0.2% antibiotic (ascomix-s®, one kilogram of which contains lincomycin hydrochloride 44 g and sulfamethazine 110 g or 0.25% herbal formulaPROVE 1, combined with five levels of Stevia supplementation in the diets on digestibility of pigs. Two factors; 1 type of drug (0.2% antibiotic and 0.25% herbal formula PROVE 1 and 2 five Stevia levels (0,0.2, 0.4, 0.6 and 0.8% were investigated and 10 dietary treatments were used in this study. Ten related growing crossbred (Large White x Landrace barrow pigs (30±1.5 kg body weight were raised in individualmetabolism cages for three collecting periods (30, 40 and 50 kg body weight, each pig was fed one experimental diet throughout the collecting period.The results showed that pigs fed diet with either 0.2% antibiotic or 0.25% herbal formula PROVE 1 had similar digestibility of diet, crude protein (CP, fiber, ash and nitrogen free extract (NFE (89.01 vs 87.83,94.96 vs 94.23, 60.73 vs 59.03, 61.22 vs 60.44 and 93.28 vs 92.03%, respectively. Negligible differences were observed between 0 and 0.4% Stevia supplementation in diet, but levels showed better digestibility than the other levels of Stevia supplementation, and the diet with 0.4% Stevia supplementation had the highestdigestibility of diet, CP, fiber, ash and NFE (91.04, 96.43, 69.48, 70.47 and 94.07%, respectively. The diet with antibiotic combined with 0.4% Stevia had digestibility of diet, CP, fat and fiber better than the otherlevels of Stevia supplementation, especially digestibility of ash, which was significantly higher than that of diet with 0.2% Stevia, but not significantly different from the other levels of Stevia supplementation. A partof herbal formula PROVE1 combined with 0% Stevia had the highest digestibility of ash (72.90%, significantly higher than the other levels of Stevia supplementation, except the diet with herbal formula PROVE 1combined with 0.4% Stevia supplementation

  20. Trypanocidal and leishmanicidal activities of flavonoids isolated from Stevia satureiifolia var. satureiifolia.

    Science.gov (United States)

    Beer, María Florencia; Frank, Fernanda Maria; Germán Elso, Orlando; Ernesto Bivona, Augusto; Cerny, Natacha; Giberti, Gustavo; Luis Malchiodi, Emilio; Susana Martino, Virginia; Alonso, María Rosario; Patricia Sülsen, Valeria; Cazorla, Silvia Ines

    2016-10-01

    Context Chagas' disease and leishmaniasis produce significant disability and mortality with great social and economic impact. The genus Stevia (Asteraceae) is a potential source of antiprotozoal compounds. Objective Aerial parts of four Stevia species were screened on Trypanosoma cruzi. Stevia satureiifolia (Lam.) Sch. Bip. var. satureiifolia (Asteraceae) dichloromethane extract was selected for a bioassay-guided fractionation in order to isolate its active compounds. Additionally, the antileishmanial activity and the cytotoxicity of these compounds on mammalian cells were assessed. Materials and methods The dichloromethane extract was fractionated by column chromatography. The isolated compounds were evaluated using concentrations of 0-100 μg/mL on T. cruzi epimastigotes and on Leishmania braziliensis promastigotes for 72 h, on trypomastigotes and amastigotes of T. cruzi for 24 h and 120 h, respectively. The compounds' cytotoxicity (12.5-500 μg/mL) was assessed on Vero cells by the MTT assay. The structure elucidation of each compound was performed by spectroscopic methods and HPLC analysis. Results The dichloromethane extracts of Stevia species showed significant activity on T. cruzi epimastigotes. The flavonoids eupatorin (1.3%), cirsimaritin (1.9%) and 5-desmethylsinensetin (1.5%) were isolated from S. satureiifolia var. satureiifolia extract. Eupatorin and 5-desmethylsinensetin showed IC50 values of 0.2 and 0.4 μg/mL on T. cruzi epimastigotes and 61.8 and 75.1 μg/mL on trypomastigotes, respectively. The flavonoid 5-desmethylsinensetin showed moderate activity against T. cruzi amastigotes (IC50  value = 78.7 μg/mL) and was the most active compound on L. braziliensis promastigotes (IC50  value = 37.0 μg/mL). Neither of the flavonoids showed cytotoxicity on Vero cells, up to a concentration of 500 μg/mL.

  1. Utilization of Bagasse Fly Ash to Remove the Unpleasant Odor of Stevia Extract and Soy Milk

    Science.gov (United States)

    Ashadi; Masykuri, M.; Haryono

    2017-04-01

    Stevia is a safe natural sweetener that, but has a slightly unpleasant odor. Soy milk is undoubtedly high nutritional value, soy milk slightly unpleasant odor. Bagasse Fly Ash (BFA) is a sugar factory waste which is abundant, not widely used yet, and allowed to accumulate around the sugar factory. BFA can be activated with a solution of NaOH become adsorbent. Utilization of activated BFA to remove the odor of stevia extract and soy milk means the utilization of a waste to reduce other waste. Deodorizing done by batch system. Before being used as adsorbent, BFA characterized using SEM, XRD, FTIR, and AAS. Odor and color analysis conducted by organoleptic. The results shown activation increases the cavity, BFA containing SiO2 and Al2O3, does not contain Pb, Cr, Cd. The results shown that the BFA can reduce odor of stevia from a scale of 4 to 2, the color becomes more clear, unpleasant odor of soy milk is also reduced.

  2. Deficiência de macronutrientes em estévia: sintomas visuais e efeitos no crescimento, composição química e produção de esteviosídeo Macronutrient deficiencies in stevia: visual symptoms and effects on growth, chemical composition, and stevioside production

    Directory of Open Access Journals (Sweden)

    Marley Marico Utumi

    1999-06-01

    Full Text Available Conduziu-se experimento objetivando descrever sintomas visuais de deficiências de macronutrientes em estévia (Stevia rebaudiana e avaliar seus efeitos no crescimento, composição química e produção de esteviosídeo. Os sintomas foram: clorose generalizada, com -N; folhas verde-escuras, com -P; folhas com clorose, bronzeamento e necrose, com -K; necrose de ápices, com -Ca; clorose e necrose foliar, em "V" invertido, com -Mg e folhas verde-pálidas e menores, com -S. As deficiências de N, K e Mg reduziram o crescimento das folhas e a parte comercializável da planta, enquanto que a deficiência de Mg promoveu acentuada redução no desenvolvimento do sistema radicular. As deficiências de N, P, K e S diminuíram a relação entre matéria seca da parte aérea e das raízes, enquanto a deficiência de Mg aumentou-a. Todas as deficiências causaram a diminuição na absorção de macronutrientes, exceto a de Ca, que reduziu somente a absorção de Ca, e a de K, que não alterou as absorções de Mg e S. A composição química dos cinco últimos pares de folhas totalmente expandidas representou bem o estado nutricional da planta. As deficiências de K, Ca e S reduziram somente o teor de esteviosídeo, enquanto todas as deficiências, exceto a de P, diminuíram o conteúdo de esteviosídeo.This experiment was carried out to describe visual symptoms of macronutrients deficiencies in Stevia rebaudiana, and the effects of these deficiencies on growth, chemical composition and stevioside production. The symptoms were: yellowing with -N; dark green leaves with -P; clorotic, mottled, and necrotic leaves, with -K; apical necrosis, with -Ca; leaves with clorosis and necrosis in inverted "V" shape, with -Mg, and small pale green leaves, with -S. N, K, and Mg deficiencies reduced the leaf growth, therefore the plant marketable part. Mg deficiency caused greater reduction on the development of the radicular system. N, P, K, and S deficiencies decreasead

  3. Evaluación de la utilización de stevia en yogurt / Evaluation of the use of stevia in yogurt

    OpenAIRE

    Galvis López, Elizabeth

    2009-01-01

    El objetivo de este trabajo fue evaluar la influencia que tiene la adición de los edulcorantes stevia y/o sacarosa en la elaboración de yogurt, y la influencia que tiene la adición de cultivo probiótico en las propiedades fisicoquímicas y sensoriales del yogurt. Para tal fin se hizo un diseño de experimentos con un arreglo factorial de 2*3, dos cultivos: cultivo iniciador y cultivo iniciador con probiótico. Para hacer la inoculación de la leche se usó como cultivo iniciador el YO-MIX® 205 LY...

  4. Phylogeny and biogeography of the genus Stevia (Asteraceae: Eupatorieae): an example of diversification in the Asteraceae in the new world.

    Science.gov (United States)

    Soejima, Akiko; Tanabe, Akifumi S; Takayama, Izumi; Kawahara, Takayuki; Watanabe, Kuniaki; Nakazawa, Miyuki; Mishima, Misako; Yahara, Tetsukazu

    2017-11-01

    The genus Stevia comprises approximately 200 species, which are distributed in North and South America, and are representative of the species diversity of the Asteraceae in the New World. We reconstructed the phylogenetic relationships using sequences of ITS and cpDNA and estimated the divergence times of the major clade of this genus. Our results suggested that Stevia originated in Mexico 7.0-7.3 million years ago (Mya). Two large clades, one with shrub species and another with herb species, were separated at about 6.6 Mya. The phylogenetic reconstruction suggested that an ancestor of Stevia was a small shrub in temperate pine-oak forests and the evolutionary change from a shrub state to a herb state occurred only once. A Brazilian clade was nested in a Mexican herb clade, and its origin was estimated to be 5.2 Mya, suggesting that the migration from North America to South America occurred after the formation of the Isthmus of Panama. The species diversity in Mexico appears to reflect the habitat diversity within the temperate pine-oak forest zone. The presence of many conspecific diploid-polyploid clades in the phylogenetic tree reflects the high frequency of polyploidization among the perennial Stevia species.

  5. Natural sweetener

    African Journals Online (AJOL)

    Department of Medicinal Chemistry and Pharmacognosy University of Illinois at. Chicago USA. 2002; 18-39. 44. Ramesh K, Singh V and NW Megeji Cultivation of Stevia rebaudiana (Bert.): A comprehensive review. Rita Elkins MH. 1997. Stevia nature's sweetener (Eds). Stevia. Woodland Publishing, Inc. 2006; 160: 1-29.

  6. Effect of different drying techniques on the volatile compounds ...

    African Journals Online (AJOL)

    Purpose: To examine the volatile compounds, thermal stability and morphological characteristics of stevia (Stevia rebaudiana Bertoni) leaves after sun, oven and microwave drying. Methods: Gas chromatography-mass spectrometry with a spectral analysis manager was used to separate the volatile compounds. Dried stevia ...

  7. THE ROLL OF SOME PLANT GROWTH REGULATORS ON SHOOTS MULTIPLICATION OF STEVIA PLANTS IN VITRO

    National Research Council Canada - National Science Library

    O M Al-Obaidy; H S M Khierallah

    2017-01-01

    ... خير اهلل عال موجد عبد الزىرة العبيدي استاذ مساعد باحثة جامعة بغداد –كمية الزراعة –وحدة أبحاث النخيل والتمور khierallah70@yahoo.com المستخلص خارج الجسم Stevia...

  8. THE ROLL OF SOME PLANT GROWTH REGULATORS ON SHOOTS MULTIPLICATION OF STEVIA PLANTS IN VITRO

    National Research Council Canada - National Science Library

    O M Al-Obaidy; H S M Khierallah

    2017-01-01

    ...العبيدي وخير هللا 2017/ 48 (5) 1168 :-1158 –هجلة العلوم السراعية العراقية 1158 خارج الجسم الحي Steviaنباتتضاعف أفرع بعض منظمات النمو في دور حسام سعد الدين محمد...

  9. Comparison of spray, freeze and oven drying as a means of reducing bitter aftertaste of steviol glycosides (derived from Stevia rebaudiana Bertoni plant)--Evaluation of the final products.

    Science.gov (United States)

    Chranioti, Charikleia; Chanioti, Sofia; Tzia, Constantina

    2016-01-01

    The aim of this study was to encapsulate the steviol glycosides (SGs) by spray, freeze and vacuum oven drying in order to minimise the bitter aftertaste of the SGs, as well as to improve their properties. Different ratios of maltodextrin to inulin as agents were examined, 80:20, 75:25, 70:30, 65:35 and 60:40, while the concentration of SGs in total solids was maintained constant at 2.5%. The obtained SGs products were evaluated by microencapsulation efficiency (MEE%), hygroscopicity, solubility, moisture content, structure (SEM, XRD), FT-IR and sensory properties. Significant differences in MEE%, moisture content, structure and solubility values were observed depending on the applied drying method. The reduced hygroscopicity values (20.26-26.67 g H2O/100g dry weight) contribute to improved stability. The FT-IR technique confirmed that SGs maintained their chemical integrity during the applied drying processes. The spray dried SGs products presented the best physicochemical characteristics and the most appealing sensorial ones. Copyright © 2015 Elsevier Ltd. All rights reserved.

  10. Sweetener Intake by Rats Selectively Bred for Differential Saccharin Intake: Sucralose, Stevia, and Acesulfame Potassium.

    Science.gov (United States)

    Dess, Nancy K; Dobson, Kiana; Roberts, Brandon T; Chapman, Clinton D

    2017-06-01

    Behavioral responses to sweeteners have been used to study the evolution, mechanisms, and functions of taste. Occidental low and high saccharin consuming rats (respectively, LoS and HiS) have been selectively outbred on the basis of saccharin intake and are a valuable tool for studying variation among individuals in sweetener intake and its correlates. Relative to HiS rats, LoS rats consume smaller amounts of all nutritive and nonnutritive sweeteners tested to date, except aspartame. The lines also differ in intake of the commercial product Splenda; the roles of sucralose and saccharides in the difference are unclear. The present study extends prior work by examining intake of custom mixtures of sucralose, maltodextrin, and sugars and Splenda by LoS and HiS rats (Experiment 1A-1D), stevia and a constituent compound (rebaudioside A; Experiment 2A-2E), and acesulfame potassium tested at several concentrations or with 4 other sweeteners at one concentration each (Experiment 3A-3B). Results indicate that aversive side tastes limit intake of Splenda, stevia, and acesulfame potassium, more so among LoS rats than among HiS rats. In addition, regression analyses involving 5 sweeteners support the idea that both sweetness and bitterness are needed to account for intake of nonnutritive sweeteners, more so among LoS rats. These findings contribute to well developed and emerging literatures on sweetness and domain-general processes related to gustation. © The Author 2017. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  11. Introducción en Guayaquil del yogurt artesanal hecho de arazá con stevia, con fines medicinales

    OpenAIRE

    Loma Iturralde, David Antenor; Castellanos Espinoza, Esther Briggitte; Elizabeth Pagalo, Carolina

    2009-01-01

    This article contains the production and marketing of artisanal yogurt made with fruit is also called Araza and sweetened with a plant called Stevia, which help people suffering from diabetes and hypertension which is intended as a contribution to society with a new trend of yogurt. We will create loyalty among consumers, giving a product of good taste and quality. It develops a marketing plan for greater market share. Furthermore, we analyze a technical study seeking the feasibility of produ...

  12. Evaluation of the risk of fungal spoilage when substituting sucrose with commercial purified Stevia glycosides in sweetened bakery products.

    Science.gov (United States)

    Rodríguez, Alicia; Magan, Naresh; Medina, Angel

    2016-08-16

    The objectives of this study were to compare the effect of different Stevia-based sugar substitutes (S1-S3), sucrose alone and a mixture of sucrose+S1 on: (a) humectant properties, (b) relative colonisation rates of sponge cake slices at 0.90 aw by strains of Aspergillus flavus, Eurotium amstelodami, Fusarium graminearum and Penicillium verrucosum at 20 and 25°C and (c) shelf-life periods in days prior to visible growth. Results showed that sucrose, S1 commercial sugar substitute and the mixture of sucrose+S1 in water solutions were able to reach water activity levels similar to those of glycerol and glucose mixtures. The S2 and S3 commercial sugar substitutes were unable to reduce aw levels significantly. At 25°C, colonisation of sponge cake slices by E. amstelodami, A. flavus and P. verrucosum occurred in all the treatments. Growth of F. graminearum only occurred on sponge cake slices containing S2 and S3 Stevia-based products at both temperatures. The best control of growth (30days) was achieved in cake slices modified with sucrose or S1 Stevia treatments inoculated with A. flavus and in the sucrose treatment for E. amstelodami at 20°C. F. graminearum growth was completely inhibited when sucrose alone, S1 or sucrose+S1 treatments were used at both temperatures. This study suggests that, as part of a hurdle technology approach, replacing sucrose with low calorie sugar substitutes based on Stevia glycosides needs to be done with care. This is because different products may have variable humectant properties and bulking agents which may shorten the potential shelf-life of intermediate moisture bakery products. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. No Effect of Dietary Aspartame or Stevia on Pancreatic Acinar Carcinoma Development, Growth, or Induced Mortality in a Murine Model.

    Science.gov (United States)

    Dooley, James; Lagou, Vasiliki; Dresselaers, Tom; van Dongen, Katinka A; Himmelreich, Uwe; Liston, Adrian

    2017-01-01

    Pancreatic cancer has an extremely poor prognosis, largely due to a poor record for early detection. Known risk factors for pancreatic cancer include obesity, diet, and diabetes, implicating glucose consumption and regulation as a key player. The role of artificial sweeteners may therefore be pertinent to disease kinetics. The oncogenic impact of artificial sweeteners is a highly controversial area. Aspartame, one of the most studied food additives, is widely recognized as being generally safe, although there are still specific areas where research is incomplete due to study limitations. Stevia, by contrast, has been the subject of relatively few studies, and the potential health benefits are based on extrapolation rather than direct testing. Here, we used longitudinal tracking of pancreatic acinar carcinoma development, growth, and lethality in a sensitized mouse model. Despite exposure to aspartame and stevia from the in utero stage onward, we found no disease modification activity, in either direction. These results contribute to the data on aspartame and stevia safety, while also reducing confidence in several of the purported health benefits.

  14. No Effect of Dietary Aspartame or Stevia on Pancreatic Acinar Carcinoma Development, Growth, or Induced Mortality in a Murine Model

    Science.gov (United States)

    Dooley, James; Lagou, Vasiliki; Dresselaers, Tom; van Dongen, Katinka A.; Himmelreich, Uwe; Liston, Adrian

    2017-01-01

    Pancreatic cancer has an extremely poor prognosis, largely due to a poor record for early detection. Known risk factors for pancreatic cancer include obesity, diet, and diabetes, implicating glucose consumption and regulation as a key player. The role of artificial sweeteners may therefore be pertinent to disease kinetics. The oncogenic impact of artificial sweeteners is a highly controversial area. Aspartame, one of the most studied food additives, is widely recognized as being generally safe, although there are still specific areas where research is incomplete due to study limitations. Stevia, by contrast, has been the subject of relatively few studies, and the potential health benefits are based on extrapolation rather than direct testing. Here, we used longitudinal tracking of pancreatic acinar carcinoma development, growth, and lethality in a sensitized mouse model. Despite exposure to aspartame and stevia from the in utero stage onward, we found no disease modification activity, in either direction. These results contribute to the data on aspartame and stevia safety, while also reducing confidence in several of the purported health benefits. PMID:28232906

  15. The effect of temperature and flow rate on the clarification of the aqueous stevia-extract in a fixed-bed column with zeolites

    Directory of Open Access Journals (Sweden)

    Mantovaneli I. C. C.

    2004-01-01

    Full Text Available Stevia is being used as a sweetener due to its low calorific value and its taste, which is very similar to that of sucrose. After extraction from dried leaves, stevia extract is dark in colour so needs to be clarified for better acceptance by consumers. Adsorption is one of the most important processes in this clarification. In this work the clarification of extract stevia extract in fixed-bed columns with calcium zeolites was studied. Two temperatures (10ºC and 30ºC and six different flow rates (2, 5, 9, 12, 16 and 19 mL/min were studied. The results showed that the mass-transfer coeffcient increases with an increase in flow rate and the length of unused bed reaches a maximum at 9 mL/min for both temperatures. The fit of the Thomas model with the breakthrough data was not very good.

  16. Evaluation of the Addition of Oats, Mango and Stevia in Yogurt Made from Mixtures of Goat’s and Cow’s Semi-Skimmed Milk

    Directory of Open Access Journals (Sweden)

    Ricardo Adolfo Parra Huertas

    2015-07-01

    Full Text Available Product development is a sequential process of finding innovative ideas in food and turn them into successful products, i.e., that are safe, provide benefits to the health of consumers and are an alternative food for the consumers. Such is the case of yogurt with goat’s and cow`s semi-skimmed milk, oats, mango and stevia. A mixture of goat’s and cow’s semi-skimmed milk with oats, mango and stevia was selected for the production of a yogurt. The objective of this research was to develop a yogurt with goat’s and cow’s semi-skimmed milk, using oats, mango y stevia evaluating their effect on the physical, chemical, structural, nutritional and sensorial characteristics during storage, while drawing the added value provided by goat’s and cow’s semi-skimmed milk, oats, mango and stevia as a sweetener and its use in industry. The yogurt was made taking into consideration three mixtures of goat and cow milk 90 %-10 %, 70 %-30 %-50 %-50 % respectively, sensory analysis determined that the best mix corresponded to 70 %-30 %. Eight formulations 70 %-30 % of yogurt were prepared by combining oats, mango and stevia at different concentrations before and during incubation. The experiment was 15 days long and the pH, acidity and syneresis were evaluated. The best formulation was selected and compared to a mixture of goat`s and cow`s semi-skimmed milk with sucrose. The physicochemical, microbiological, proximal, sensory and structural characteristics were determined for both treatments during 30 days. The results suggest that the addition of 3 % oats, 5 % mango and 2 % stevia to a 70/30 mixture of milk is favorable in the preparation of the yogurt.

  17. Effect of Incorporating Stevia and Moringa in Cookies on Postprandial Glycemia, Appetite, Palatability, and Gastrointestinal Well-Being.

    Science.gov (United States)

    Ahmad, Jamil; Khan, Imran; Johnson, Stuart K; Alam, Iftikhar; Din, Zia Ud

    2018-02-01

    Medicinal plants including stevia and moringa constitute an important source of health-beneficial bioactive components, and hence their intake may beneficially modulate biomarkers of chronic diseases. The objective of the present study was to investigate the effect of incorporating stevia and moringa leaf powder in cookies on postprandial glycemia, appetite, palatability, and gastrointestinal well-being in humans. In a randomized crossover design, 20 healthy subjects consumed 3 isocaloric test foods (each providing 50 g available carbohydrates) of control cookies (CC) made from 100% wheat flour, cookies containing stevia leaf powder (SC, 3% w/w), and cookies containing moringa leaf powder (MC, 5% w/w) as breakfast. Blood glucose and subjective appetite were measured at fasting and at 15, 30, 45, 60, 90, and 120 min after the consumption of the cookies. Palatability and gastrointestinal well-being were measured using standard questionnaires. Compared to CC, MC resulted in a significant decrease in postprandial blood glucose concentration at 30 and 45 min (p = 0.002 and p = 0.003, respectively) and showed a tendency (p = 0.077) for lower blood glucose incremental area under the curve (iAUC). Subjects were significantly less hungry after SC and MC intake (p = 0.035 and p = 0.041, respectively) compared to CC. All the cookies were liked by the subjects without any reported gastrointestinal discomfort. The results showed that compared to CC, MC improved postprandial glycemia and reduced hunger, while SC reduced hunger only. Future studies are now warranted to explore the mechanisms responsible for these observed effects.

  18. African Journal of Biotechnology - Vol 5, No 13 (2006)

    African Journals Online (AJOL)

    In vitro propagation of Stevia rebaudiana Bert in Bangladesh · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. MS Uddin, MSH Chowdhury, MMMH Khan, MB Uddin, R Ahmed, MdA Baten ...

  19. Hybridization of polyvinylpyrrolidone to a binary composite of curcumin/α-glucosyl stevia improves both oral absorption and photochemical stability of curcumin.

    Science.gov (United States)

    Kadota, Kazunori; Okamoto, Daiki; Sato, Hideyuki; Onoue, Satomi; Otsu, Shigeyuki; Tozuka, Yuichi

    2016-12-15

    The tri-component system curcumin/α-glucosyl stevia (Stevia-G)/polyvinylpyrrolidone (PVP) was developed to improve the oral bioavailability and physicochemical properties of curcumin (CUR). The tri-component CUR formulation with Stevia-G and PVP was prepared with freeze-drying. The tri-component CUR system exhibited 13,000-fold higher solubility of CUR than the equilibrium solubility of CUR for 24h, indicating a stable tri-composite structure involving CUR. CUR could be converted into an amorphous form in the presence of Stevia-G and PVP by freeze-drying. The photo-degradation of CUR in the tri-component system was negligible even under an amorphous state of CUR. After oral administration in rats, the oral absorption of the tri-component CUR formulation (20mgCUR/kg) was 6.7-fold higher than that of crystalline CUR. The tri-component CUR formulation would therefore be a promising option to improve physicochemical properties and oral absorption of CUR. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Effect of steviol, steviol glycosides and stevia extract on glucocorticoid receptor signaling in normal and cancer blood cells.

    Science.gov (United States)

    Panagiotou, Christina; Mihailidou, Chrysovalantou; Brauhli, George; Katsarou, Olga; Moutsatsou, Paraskevi

    2017-07-25

    The use of steviol glycosides as non-caloric sweeteners has proven to be beneficial for patients with type 2 diabetes mellitus (T2D), obesity, and metabolic syndrome. However, recent data demonstrate that steviol and stevioside might act as glucocorticoid receptor (GR) agonists and thus correlate with adverse effects on metabolism. Herein, we evaluated the impact of steviol, steviol glycosides, and a Greek-derived stevia extract on a number of key steps of GR signaling cascade in peripheral blood mononuclear cells (PBMCs) and in Jurkat leukemia cells. Our results revealed that none of the tested compounds altered the expression of primary GR-target genes (GILZ, FKPB5), GR protein levels or GR subcellular localization in PBMCs; those compounds increased GILZ and FKPB5 mRNA levels as well as GRE-mediated luciferase activity, inducing in parallel GR nuclear translocation in Jurkat cells. The GR-modulatory activity demonstrated by stevia-compounds in Jurkat cells but not in PBMCs may be due to a cell-type specific effect. Copyright © 2017. Published by Elsevier B.V.

  1. Parents' and children's acceptance of skim chocolate milks sweetened by monk fruit and stevia leaf extracts.

    Science.gov (United States)

    Li, X E; Lopetcharat, K; Drake, M A

    2015-05-01

    Chocolate milk increases milk consumption of children, but high sugar content raises health concerns. Interest in sugar reduction and parents' preference for natural sweeteners necessitates further research on natural nonnutritive sweeteners. However, it is important to maintain consumer acceptability, especially for children, while reducing sugar in chocolate milk. The objectives of this study were to identify the sweetness intensity perception of stevia leaf (STV) and monk fruit (MK) extracts in skim chocolate milk (SCM), to evaluate STV and MK as the sole or partial sweetener source for SCM for young adults (19 to 35 y) and children (5 to 13 y), and to determine if information on natural nonnutritive sweeteners impacted parents' acceptability of SCM. Power function and 2-alternative forced choice studies were used to determine the iso-sweetness of nonnutritive sweeteners to a sucrose control in SCM (51.4 g/L, SUC control). Young adults (n = 131) evaluated 9 different SCM (SUC control, STV, MK, STV:sucrose blends, or MK:sucrose blends) in a completely randomized 2-d test. Children (n = 167) evaluated SUC control SCM and SCM with 39.7 g/L sucrose and 46 mg/L MK (MK25) or 30 mg/L STV (STV25). Parents evaluated SUC control, MK25, and STV25 in a balanced crossover design with a 40-d wait time between primed or unprimed ballots. Chocolate milks solely sweetened by nonnutritive sweeteners were less acceptable compared with SUC control by young adults. MK25 and STV25 were acceptable by young adults and children. The presentation of chocolate milk label information had different effects on parental acceptance. Traditional parents preferred sucrose sweetened SCM, and label conscious parents preferred SCM with natural nonnutritive sweeteners. © 2015 Institute of Food Technologists®

  2. Tropical Journal of Pharmaceutical Research - Vol 12, No 6 (2013)

    African Journals Online (AJOL)

    Influence of Salicylic Acid on the Antimicrobial Potential of Stevia (Stevia rebaudiana Bertoni, Asteraceae) Leaf Extracts against Soybean Seed-Borne Pathogens · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. M Sedghi, S Gholi-Toluie, 1035-1038.

  3. EFFECT OF EXPALNT TYPE AND SOME PLANT GROWTH REGULATORS ON CULTURE INITIATION OF STEVIA PLANTS IN VITRO

    National Research Council Canada - National Science Library

    H S M Khierallah; O M A Al-Obaidy

    2017-01-01

    ...خير هللا والعبيدي 2017/ 48 (5) 1214 :-1206 –هجلة العلوم الزراعية العراقية 1206 خارج الجسم الحي Steviaنبات الجزء النباتي وبعض منظمات النمو في نشوء زروعات تأثير...

  4. Pengaruh Penambahan Ekstrak Kayu Secang Dan Ekstrak Daun Stevia Terhadap Aktivitas Antioksidan Dan Kadar Gula Total Pada Yoghurt Sebagai Alternatif Minuman Bagi Penderita Diabetes Melitus Tipe 2

    OpenAIRE

    Umami, Chomsatun; Afifah, Diana Nur

    2015-01-01

    Latar Belakang: Penatalaksanaan diet diabetes melitus tipe 2 dapat dilakukan dengan meningkatkan asupan antioksidan serta memperhatikan jumlah dan jenis karbohidrat yang dikonsumsi terutama asupan gula sederhana. Yoghurt, ekstrak kayu secang, dan ekstrak daun stevia merupakan bahan alami tinggi antioksidan dan rendah gula total yang dapat dijadikan pangan fungsional sebagai alternatif minuman untuk penderita DM tipe 2.Tujuan: Menganalisis pengaruh penambahan ekstrak kayu secang dan ekstrak da...

  5. Development and application of a quantitative method based on LC-QqQ MS/MS for determination of steviol glycosides in Stevia leaves.

    Science.gov (United States)

    Molina-Calle, M; Sánchez de Medina, V; Delgado de la Torre, M P; Priego-Capote, F; Luque de Castro, M D

    2016-07-01

    Stevia is a currently well-known plant thanks to the presence of steviol glycosides, which are considered as sweeteners obtained from a natural source. In this research, a method based on LC-MS/MS by using a triple quadrupole detector was developed for quantitation of 8 steviol glycosides in extracts from Stevia leaves. The ionization and fragmentation parameters for selected reaction monitoring were optimized. Detection and quantitation limits ranging from 0.1 to 0.5ng/mL and from 0.5 to 1ng/mL, respectively, were achieved: the lowest attained so far. The steviol glycosides were quantified in extracts from leaves of seven varieties of Stevia cultivated in laboratory, greenhouse and field. Plants cultivated in field presented higher concentration of steviol glycosides than those cultivated in greenhouse. Thus, the way of cultivation clearly influences the concentration of these compounds. The inclusion of branches together with leaves as raw material was also evaluated, showing that this inclusion modifies, either positively or negatively, the concentration of steviol glycosides. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. Stevia-derived compounds attenuate the toxic effects of ectopic lipid accumulation in the liver of obese mice: a transcriptomic and metabolomic study.

    Science.gov (United States)

    Holvoet, Paul; Rull, Anna; García-Heredia, Anabel; López-Sanromà, Sílvia; Geeraert, Benjamine; Joven, Jorge; Camps, Jordi

    2015-03-01

    There is a close interaction between Type 2 Diabetes, obesity and liver disease. We have studied the effects of the two most abundant Stevia-derived steviol glycosides, stevioside and rebaudioside A, and their aglycol derivative steviol on liver steatosis and the hepatic effects of lipotoxicity using a mouse model of obesity and insulin resistance. We treated ob/ob and LDLR-double deficient mice with stevioside (10 mg⋅kg(-1)⋅day-1 p.o., n = 8), rebaudioside A (12 mg⋅kg(-1)⋅day-1 p.o., n = 8), or steviol (5 mg⋅kg(-1)⋅day(-1) p.o., n = 8). We determined their effects on liver steatosis and on the metabolic effects of lipotoxicity by histological analysis, and by combined gene-expression and metabolomic analyses. All compounds attenuated hepatic steatosis. This could be explained by improved glucose metabolism, fat catabolism, bile acid metabolism, and lipid storage and transport. We identified PPARs as important regulators and observed differences in effects on insulin resistance, inflammation and oxidative stress between Stevia-derived compounds. We conclude that Stevia-derived compounds reduce hepatic steatosis to a similar extent, despite differences in effects on glucose and lipid metabolism, and inflammation and oxidative stress. Thus our data show that liver toxicity can be reduced through several pathophysiological changes. Further identification of active metabolites and underlying mechanisms are warranted. Copyright © 2014 Elsevier Ltd. All rights reserved.

  7. The influence of stevia on the flow, shear and compression behavior of sorbitol, a pharmaceutical excipient for direct compression.

    Science.gov (United States)

    Hurychová, Hana; Ondrejček, Pavel; Šklubalová, Zdenka; Vraníková, Barbora; Svěrák, Tomáš

    2018-02-01

    Good flow and compaction properties are necessary for the manipulation of particulate material in the pharmaceutical industry. The influence of the addition of an alternative sweetener, rebaudioside A, in a concentration 0.2% w/w and 0.5% w/w on the flow, shear and compaction properties of sorbitol for direct compaction, Merisorb® 200, was investigated in this work. Rebaudioside A worsened the flow properties of sorbitol: the Hausner ratio, the compressibility index and the mass flow rate through the aperture of a model hopper. Using a Jenike shear cell revealed a significant increase in cohesion leading to the decrease of the flow function; moreover, the addition of rebaudioside A increased the total energy for compression of tablets and plasticity estimated by the force-displacement method. Finally, the tablets showed a higher tensile strength and needed longer time to disintegrate compared to the tablets made of sorbitol itself. In view of the results for the free-flowable excipient, sorbitol, the effects of stevia even for a 0.2% w/w concentration have to be carefully considered, particularly whenever used in pharmaceutical formulations of poor flow properties.

  8. Effects of aspartame-, monk fruit-, stevia- and sucrose-sweetened beverages on postprandial glucose, insulin and energy intake.

    Science.gov (United States)

    Tey, S L; Salleh, N B; Henry, J; Forde, C G

    2017-03-01

    Substituting sweeteners with non-nutritive sweeteners (NNS) may aid in glycaemic control and body weight management. Limited studies have investigated energy compensation, glycaemic and insulinaemic responses to artificial and natural NNS. This study compared the effects of consuming NNS (artificial versus natural) and sucrose (65 g) on energy intake, blood glucose and insulin responses. Thirty healthy male subjects took part in this randomised, crossover study with four treatments: aspartame-, monk fruit-, stevia- and sucrose-sweetened beverages. On each test day, participants were asked to consume a standardised breakfast in the morning, and they were provided with test beverage as a preload in mid-morning and ad libitum lunch was provided an hour after test beverage consumption. Blood glucose and insulin concentrations were measured every 15 min within the first hour of preload consumption and every 30 min for the subsequent 2 h. Participants left the study site 3 h after preload consumption and completed a food diary for the rest of the day. Ad libitum lunch intake was significantly higher for the NNS treatments compared with sucrose (P=0.010). The energy 'saved' from replacing sucrose with NNS was fully compensated for at subsequent meals; hence, no difference in total daily energy intake was found between the treatments (P=0.831). The sucrose-sweetened beverage led to large spikes in blood glucose and insulin responses within the first hour, whereas these responses were higher for all three NNS beverages following the test lunch. Thus, there were no differences in total area under the curve (AUC) for glucose (P=0.960) and insulin (P=0.216) over 3 h between the four test beverages. The consumption of calorie-free beverages sweetened with artificial and natural NNS have minimal influences on total daily energy intake, postprandial glucose and insulin compared with a sucrose-sweetened beverage.

  9. Untitled

    African Journals Online (AJOL)

    rial potential in Parthenium hysterophorus, Stevia rebaudiana and Ginkgo biloba. Pak. J. Bot., 43(2), 1307-1313. Hailamariam, A., Haile, G., and Harikrishna, R., 2015. Effeciancy of Parthenium hys- terophorus against cockroaches in Aksum University, Tigray region, Northern. Ethiopia. Int. J. Sci. Innov. Techno., 4, 7-10.

  10. Aldose Reductase Inhibitory and Antiglycation Activities of Four ...

    African Journals Online (AJOL)

    Thonn., Punica granatum L., and Stevia rebaudiana Bertoni) standardized extracts and their major constituents (morusin, phyllanthin, punicalagin and stevioside) in the treatment of long-term diabetic complications by inhibition of aldose reductase (AR) enzyme and advanced glycation end products (AGEs) formation.

  11. Journal of Genetics | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    More Details Fulltext PDF. Volume 93 Online resources 2014 pp e75-e81. Functionally relevant novel microsatellite markers for efficient genotyping in Stevia rebaudiana Bertoni · Abhishek Bhandawat Himanshu Sharma Akshay Nag Sanatsujat Singh Paramvir Singh Ahuja Ram Kumar Sharma · More Details Fulltext PDF ...

  12. Author Details

    African Journals Online (AJOL)

    ... Vol 13, No 1 (2014) - Articles Effects of Phytosterol in Feed on Growth and Related Gene Expression in Muscles of Broiler Chickens Abstract PDF · Vol 13, No 1 (2014) - Articles Nutritional Composition of Stevia rebaudiana Bertoni Leaf: Effect of Drying Method Abstract PDF. ISSN: 1596-9827. AJOL African Journals Online.

  13. Journal of Genetics | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Genetics. Sanatsujat Singh. Articles written in Journal of Genetics. Volume 93 Online resources 2014 pp e75-e81. Functionally relevant novel microsatellite markers for efficient genotyping in Stevia rebaudiana Bertoni · Abhishek Bhandawat Himanshu Sharma Akshay Nag Sanatsujat Singh ...

  14. simulation du climat futur et des rendements agricoles en region ...

    African Journals Online (AJOL)

    ACSS

    steviosides produced from Stevia rebaudiana Bertoni plant. African Journal of Food Science 4(5):269-281. FAO. 1978. Forestry for Local Community. Development Food and Agriculture. Organization of the United Nation (FAO),. FAO Forestry paper, No 7, Rome, Italy. FAO. 1997. FAO database on introduced aquatic species ...

  15. Journal of Genetics | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Functionally relevant novel microsatellite markers for efficient genotyping in Stevia rebaudiana Bertoni · Abhishek Bhandawat Himanshu Sharma Akshay Nag Sanatsujat Singh Paramvir Singh Ahuja Ram Kumar Sharma · More Details Fulltext PDF. Volume 94 Issue 4 December 2015 pp 697-704 Research Article.

  16. Author Details

    African Journals Online (AJOL)

    Hua, Xiao. Vol 16, No 6 (2017) - Articles Effect of different drying techniques on the volatile compounds, morphological characteristics and thermal stability of Stevia rebaudiana Bertoni leaf. Abstract PDF. ISSN: 1596-9827. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors ...

  17. Author Details

    African Journals Online (AJOL)

    Illanchezian, S. Vol 7, No 4 (2008) - Articles In-vitro Antimicrobial and Antitumor Activities of Stevia Rebaudiana (Asteraceae) Leaf Extracts Abstract PDF. ISSN: 1596-9827. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors. OTHER RESOURCES... Journal Quality · for ...

  18. Author Details

    African Journals Online (AJOL)

    Tadhani, M B. Vol 5, No 1 (2006) - Articles In Vitro Antimicrobial Activity of Stevia Rebaudiana Bertoni Leaves Abstract PDF. ISSN: 1596-9827. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of Use ...

  19. Tropical Journal of Pharmaceutical Research - Vol 7, No 4 (2008)

    African Journals Online (AJOL)

    In-vitro Antimicrobial and Antitumor Activities of Stevia Rebaudiana (Asteraceae) Leaf Extracts · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. S Jayaraman, MS Manoharan, S Illanchezian, 1143-1149. http://dx.doi.org/10.4314/tjpr.v7i4.14700 ...

  20. Original Research Original Research

    African Journals Online (AJOL)

    RAGHAVENDRA

    In-vitro Antimicrobial and Antitumor Activities of Stevia. Rebaudiana (Asteraceae) Leaf Extracts. Tropical Journal of Pharmaceutical Research 7(4): 1143-1149. Kaur, J., Rathinam, X., Kasi, M., Leng, K.M., Ayyalu, R.,. Kathiresan, S. (2010). Preliminary investigation on the antibacterial activity of mango (Mangifera indica L:.

  1. Browse Title Index

    African Journals Online (AJOL)

    Items 51 - 100 of 1932 ... Vol 7, No 4 (2008), In-vitro Antimicrobial and Antitumor Activities of Stevia Rebaudiana (Asteraceae) Leaf Extracts, Abstract PDF. S Jayaraman, MS Manoharan, S Illanchezian. Vol 16, No 7 (2017), In-vitro cytotoxicity of biosynthesized gold nanoparticles against thyroid cancer cell lines, Abstract PDF.

  2. Journal of Genetics | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Functionally relevant novel microsatellite markers for efficient genotyping in Stevia rebaudiana Bertoni · Abhishek Bhandawat Himanshu Sharma Akshay Nag Sanatsujat Singh Paramvir Singh Ahuja Ram Kumar Sharma · More Details Fulltext PDF. pp e82-e85. Identification of twenty novel polymorphic microsatellite DNA ...

  3. Author Details

    African Journals Online (AJOL)

    Tadhani, M B. Vol 5, No 1 (2006) - Articles In Vitro Antimicrobial Activity of Stevia Rebaudiana Bertoni Leaves Abstract PDF · AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of Use · Contact AJOL · News.

  4. Author Details

    African Journals Online (AJOL)

    Physicochemical, Nutritional, and Functional Characteristics of Seeds, Peel and Pulp of Grewia tenax (Forssk) Fiori Fruits Abstract PDF · Vol 16, No 6 (2017) - Articles Effect of different drying techniques on the volatile compounds, morphological characteristics and thermal stability of Stevia rebaudiana Bertoni leaf

  5. Journal of Genetics | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Genetics; Volume 93; Online resources. Functionally relevant novel microsatellite markers for efficient genotyping in Stevia rebaudiana Bertoni. Abhishek Bhandawat Himanshu Sharma Akshay Nag Sanatsujat Singh Paramvir Singh Ahuja Ram Kumar Sharma. Volume 93 Online resources 2014 ...

  6. Quantification of steviol glycosides in food products, Stevia leaves and formulations by planar chromatography, including proof of absence for steviol and isosteviol.

    Science.gov (United States)

    Wald, Julian P; Morlock, Gertrud E

    2017-07-14

    Steviol glycosides may degrade in food products under certain processing and storage conditions. Hence, a method was developed that separated in the same chromatographic run seven important steviol glycosides, and additionally as a sum parameter, their reported breakdown products steviol and isosteviol. Through derivatizations with the 2-naphthol and the primuline reagent, the detection was selective and inexpensive. In case needed, the baseline separation of steviol and isosteviol was also demonstrated after a plate cut and subsequent short development (two-step method). The HPTLC method was robust with regard to varying sample matrix loads, as the stationary phase was used only once. A high sample throughput was achieved, i.e. 23 separations were performed in parallel on one plate. The total analysis time took 1h (30min application, 15min separation and 15min derivatization/densitometry) leading to a calculated analysis time of 2.6min per sample. The solvent consumption was 8mL in total (0.4mL per analysis). HPTLC-ESI-MS was employed for confirmation of the results obtained. Mass spectra were recorded only from the zones of interest, and not from matrix or background, leading to decisive advantages, such as less need for MS cleaning. The optimized HPTLC method was shown to effectively support quality control, as marketed samples may be falsified with cheaper synthetic sweeteners, which was also demonstrated in this study. The accuracy of the densitometric quantification in HPTLC was considered as high, as standards and samples were separated on fresh adsorbent and detected simultaneously under identical conditions, which minimized the influence of errors. Finally, the Aliivibrio fischeri bioassay was employed to obtain information on bioactive compounds in Stevia leaf extracts. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Steviol glycosides modulate glucose transport in different cell types.

    Science.gov (United States)

    Rizzo, Benedetta; Zambonin, Laura; Angeloni, Cristina; Leoncini, Emanuela; Dalla Sega, Francesco Vieceli; Prata, Cecilia; Fiorentini, Diana; Hrelia, Silvana

    2013-01-01

    Extracts from Stevia rebaudiana Bertoni, a plant native to Central and South America, have been used as a sweetener since ancient times. Currently, Stevia extracts are largely used as a noncaloric high-potency biosweetener alternative to sugar, due to the growing incidence of type 2 diabetes mellitus, obesity, and metabolic disorders worldwide. Despite the large number of studies on Stevia and steviol glycosides in vivo, little is reported concerning the cellular and molecular mechanisms underpinning the beneficial effects on human health. The effect of four commercial Stevia extracts on glucose transport activity was evaluated in HL-60 human leukaemia and in SH-SY5Y human neuroblastoma cells. The extracts were able to enhance glucose uptake in both cellular lines, as efficiently as insulin. Our data suggest that steviol glycosides could act by modulating GLUT translocation through the PI3K/Akt pathway since treatments with both insulin and Stevia extracts increased the phosphorylation of PI3K and Akt. Furthermore, Stevia extracts were able to revert the effect of the reduction of glucose uptake caused by methylglyoxal, an inhibitor of the insulin receptor/PI3K/Akt pathway. These results corroborate the hypothesis that Stevia extracts could mimic insulin effects modulating PI3K/Akt pathway.

  8. Steviol Glycosides Modulate Glucose Transport in Different Cell Types

    Directory of Open Access Journals (Sweden)

    Benedetta Rizzo

    2013-01-01

    Full Text Available Extracts from Stevia rebaudiana Bertoni, a plant native to Central and South America, have been used as a sweetener since ancient times. Currently, Stevia extracts are largely used as a noncaloric high-potency biosweetener alternative to sugar, due to the growing incidence of type 2 diabetes mellitus, obesity, and metabolic disorders worldwide. Despite the large number of studies on Stevia and steviol glycosides in vivo, little is reported concerning the cellular and molecular mechanisms underpinning the beneficial effects on human health. The effect of four commercial Stevia extracts on glucose transport activity was evaluated in HL-60 human leukaemia and in SH-SY5Y human neuroblastoma cells. The extracts were able to enhance glucose uptake in both cellular lines, as efficiently as insulin. Our data suggest that steviol glycosides could act by modulating GLUT translocation through the PI3K/Akt pathway since treatments with both insulin and Stevia extracts increased the phosphorylation of PI3K and Akt. Furthermore, Stevia extracts were able to revert the effect of the reduction of glucose uptake caused by methylglyoxal, an inhibitor of the insulin receptor/PI3K/Akt pathway. These results corroborate the hypothesis that Stevia extracts could mimic insulin effects modulating PI3K/Akt pathway.

  9. Steviol Glycosides Modulate Glucose Transport in Different Cell Types

    Science.gov (United States)

    Rizzo, Benedetta; Zambonin, Laura; Leoncini, Emanuela; Vieceli Dalla Sega, Francesco; Prata, Cecilia; Fiorentini, Diana; Hrelia, Silvana

    2013-01-01

    Extracts from Stevia rebaudiana Bertoni, a plant native to Central and South America, have been used as a sweetener since ancient times. Currently, Stevia extracts are largely used as a noncaloric high-potency biosweetener alternative to sugar, due to the growing incidence of type 2 diabetes mellitus, obesity, and metabolic disorders worldwide. Despite the large number of studies on Stevia and steviol glycosides in vivo, little is reported concerning the cellular and molecular mechanisms underpinning the beneficial effects on human health. The effect of four commercial Stevia extracts on glucose transport activity was evaluated in HL-60 human leukaemia and in SH-SY5Y human neuroblastoma cells. The extracts were able to enhance glucose uptake in both cellular lines, as efficiently as insulin. Our data suggest that steviol glycosides could act by modulating GLUT translocation through the PI3K/Akt pathway since treatments with both insulin and Stevia extracts increased the phosphorylation of PI3K and Akt. Furthermore, Stevia extracts were able to revert the effect of the reduction of glucose uptake caused by methylglyoxal, an inhibitor of the insulin receptor/PI3K/Akt pathway. These results corroborate the hypothesis that Stevia extracts could mimic insulin effects modulating PI3K/Akt pathway. PMID:24327825

  10. High-performance thin-layer chromatography analysis of steviol glycosides in Stevia formulations and sugar-free food products, and benchmarking with (ultra) high-performance liquid chromatography.

    Science.gov (United States)

    Morlock, Gertrud E; Meyer, Stephanie; Zimmermann, Benno F; Roussel, Jean-Marc

    2014-07-11

    A high-performance TLC (HPTLC) method was newly developed and validated for analysis of 7 steviol glycosides in 6 different types of food and Stevia formulations. After a minimized one-step sample preparation, 21 samples were developed in parallel, allowing an effective food screening. Depending on the sample application volume, the method was suited to analyze food sample concentrations in the mg/kg range. LOQs of stevioside in natural yoghurt matrix spiked at 0.02, 0.13 and 0.2% were determined by the calibration curve method to be 12ng/band (peak height). ANOVA was successfully passed to prove data homogeneity in the working range (30-600ng/band). The accuracy (recovery tolerance limit, 92-120%), repeatability (3.1-5.4%) and intermediate precision (4.0-8.4%) were determined for stevioside in milk-based matrix including sample preparation and recovery rates at 3 different concentration levels. For the first time, the recording of HPTLC-ESI-MS spectra via the TLC-MS Interface was demonstrated for rebaudioside A. HPTLC contents for rebaudioside A were compared with results of two (U)HPLC methods. The running costs and analysis time of the three different methods were discussed in detail with regard to screening of food products. Copyright © 2014 Elsevier B.V. All rights reserved.

  11. Synthesis and Sensory Evaluation of ent-Kaurane Diterpene Glycosides

    OpenAIRE

    Venkata Sai Prakash Chaturvedula; Rafael Ignacio San Miguel; Indra Prakash; Mary Campbell

    2012-01-01

    Catalytic hydrogenation of the three ent-kaurane diterpene glycosides isolated from Stevia rebaudiana, namely rubusoside, stevioside, and rebaudioside-A has been carried out using Pd(OH)2 and their corresponding dihydro derivatives have been isolated as the products. Synthesis of reduced steviol glycosides was performed using straightforward chemistry and their structures were characterized on the basis of 1D and 2D NMR spectral...

  12. Quantitation of sweet steviol glycosides by means of a HILIC-MS/MS-SIDA approach.

    Science.gov (United States)

    Well, Caroline; Frank, Oliver; Hofmann, Thomas

    2013-11-27

    Meeting the rising consumer demand for natural food ingredients, steviol glycosides, the sweet principle of Stevia rebaudiana Bertoni (Bertoni), have recently been approved as food additives in the European Union. As regulatory constraints require sensitive methods to analyze the sweet-tasting steviol glycosides in foods and beverages, a HILIC-MS/MS method was developed enabling the accurate and reliable quantitation of the major steviol glycosides stevioside, rebaudiosides A-F, steviolbioside, rubusoside, and dulcoside A by using the corresponding deuterated 16,17-dihydrosteviol glycosides as suitable internal standards. This quantitation not only enables the analysis of the individual steviol glycosides in foods and beverages but also can support the optimization of breeding and postharvest downstream processing of Stevia plants to produce preferentially sweet and least bitter tasting Stevia extracts.

  13. Synthetic polyelectrolytes in the clarification of stevia aqueous extract Aplicação de polieletrólitos sintéticos na clarificação do extrato aquoso de estévia

    Directory of Open Access Journals (Sweden)

    Élcio José Bunhak

    2002-04-01

    Full Text Available The extract of stevia leaves is usually dark which demands a clarification in order to be accepted by sweetener consumers. One of the using techniques is the decantation, which requires the use of effective products for best results. In the market, flocculant products are available, such as: NOVUSTM CE2666 (cationic and BETZDEARBORNTMF11 (anionic. When these products are used at a certain level of concentration, satisfactory results of decantation and consequent clarification are provided. The aim of this paper was to compare the performance of the best polymers by removal percentage (color and turbidity and by the decantation speed. A Jar Test device was used and the concentration of coagulant (aluminum sulfate and of polyelectrolytes were varied, as well as pH at 8,0, 9,0 and 10,0. Calcium oxide was applied in this phase. The best condition was obtained when the anionic polyelectrolyte was used, reaching a turbidity removal of 97%.O extrato das folhas de estévia são normalmente de cor escura, exigindo uma clarificação para que se apresente com aspecto aceitável frente aos consumidores de adoçantes. Dentre as técnicas utilizadas figura a decantação, a qual necessita, porém, de bons produtos para sua eficácia. No mercado estão disponíveis produtos floculantes, tais como: NOVUSTMCE2666 (catiônico e BETZDEARBORNTMF11 (aniônico, que utilizados em determinadas concentrações proporcionam resultados satisfatórios de decantação e conseqüente clarificação. O objetivo deste trabalho foi comparar através da percentagem de despigmentação (cor e turbidez e velocidade de decantação, qual o melhor polímero. Utilizando-se um aparelho de Jar Test, fez-se testes variando-se a concentração do coagulante (sulfato de alumínio e dos polieletrólitos e variou-se também o pH em 8,0, 9,0 e 10,0 aplicando-se óxido de cálcio. A melhor condição foi conseguida utilizando o polieletrólito aniônico onde atingiu-se 97% para a percentagem

  14. Computer-Aided Identification of Anticonvulsant Effect of Natural Nonnutritive Sweeteners Stevioside and Rebaudioside A.

    Science.gov (United States)

    Di Ianni, Mauricio E; Del Valle, María E; Enrique, Andrea V; Rosella, María A; Bruno, Fiorella; Bruno-Blanch, Luis E; Talevi, Alan

    2015-01-01

    Steviol glycosides are natural constituents of Stevia rebaudiana (Bert.) Bert. (Asteraceae) that have recently gained worldwide approval as nonnutritive sweeteners by the Joint Food and Agriculture Organization/World Organization Expert Committee on Food Additives. Cheminformatic tools suggested that the aglycone steviol and several of its phase I metabolites were predicted as potential anticonvulsant agents effective in the seizure animal model maximal electroshock seizure (MES) test. Thus, aqueous infusion from S. rebaudiana was tested in the MES test (mice, intraperitoneal administration), confirming dose-dependent anticonvulsant effect. Afterward, isolated stevioside and rebaudioside A were tested in the MES test, with positive results. Though drug repositioning most often focuses on known therapeutics, this article illustrates the possibilities of this strategy to find new functionalities and therapeutic indications for food constituents and natural products.

  15. Computer-Aided Identification of Anticonvulsant Effect of Natural Nonnutritive Sweeteners Stevioside and Rebaudioside A

    Science.gov (United States)

    Di Ianni, Mauricio E.; del Valle, Mara E.; Enrique, Andrea V.; Rosella, Mara A.; Bruno, Fiorella; Bruno-Blanch, Luis E.

    2015-01-01

    Abstract Steviol glycosides are natural constituents of Stevia rebaudiana (Bert.) Bert. (Asteraceae) that have recently gained worldwide approval as nonnutritive sweeteners by the Joint Food and Agriculture Organization/World Organization Expert Committee on Food Additives. Cheminformatic tools suggested that the aglycone steviol and several of its phase I metabolites were predicted as potential anticonvulsant agents effective in the seizure animal model maximal electroshock seizure (MES) test. Thus, aqueous infusion from S. rebaudiana was tested in the MES test (mice, intraperitoneal administration), confirming dose-dependent anticonvulsant effect. Afterward, isolated stevioside and rebaudioside A were tested in the MES test, with positive results. Though drug repositioning most often focuses on known therapeutics, this article illustrates the possibilities of this strategy to find new functionalities and therapeutic indications for food constituents and natural products. PMID:26258457

  16. Synthesis and Sensory Evaluation of ent-Kaurane Diterpene Glycosides

    Directory of Open Access Journals (Sweden)

    Venkata Sai Prakash Chaturvedula

    2012-07-01

    Full Text Available Catalytic hydrogenation of the three ent-kaurane diterpene glycosides isolated from Stevia rebaudiana, namely rubusoside, stevioside, and rebaudioside-A has been carried out using Pd(OH2 and their corresponding dihydro derivatives have been isolated as the products. Synthesis of reduced steviol glycosides was performed using straightforward chemistry and their structures were characterized on the basis of 1D and 2D NMR spectral data and chemical studies. Also, we report herewith the sensory evaluation of all the reduced compounds against their corresponding original steviol glycosides and sucrose for the sweetness property of these molecules.

  17. Synthesis and sensory evaluation of ent-kaurane diterpene glycosides.

    Science.gov (United States)

    Prakash, Indra; Campbell, Mary; San Miguel, Rafael Ignacio; Chaturvedula, Venkata Sai Prakash

    2012-07-26

    Catalytic hydrogenation of the three ent-kaurane diterpene glycosides isolated from Stevia rebaudiana, namely rubusoside, stevioside, and rebaudioside-A has been carried out using Pd(OH)₂ and their corresponding dihydro derivatives have been isolated as the products. Synthesis of reduced steviol glycosides was performed using straightforward chemistry and their structures were characterized on the basis of 1D and 2D NMR spectral data and chemical studies. Also, we report herewith the sensory evaluation of all the reduced compounds against their corresponding original steviol glycosides and sucrose for the sweetness property of these molecules.

  18. On the connection between nonmonotonic taste behavior and molecular conformation in solution: The case of rebaudioside-A

    Energy Technology Data Exchange (ETDEWEB)

    Chopade, Prashant D.; Sarma, Bipul; Santiso, Erik E.; Chen, Jie; Trout, Bernhardt L.; Myerson, Allan S., E-mail: myerson@mit.edu [Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, 66-568, Cambridge, Massachusetts 02139 (United States); Simpson, Jeffrey [Department of Chemistry Instrumentation Facility, Massachusetts Institute of Technology, 77 Massachusetts Avenue, 18-0090, Cambridge, Massachusetts 02139 (United States); Fry, John C.; Biermann, Kari L. [Connect Consulting, 6 Hollands Field, Horsham RH123HQ (United Kingdom); Yurttas, Nese [Cargill, Inc., Global Food Technology, 2301 Crosby Road, Wayzata, Minnesota 55391 (United States)

    2015-12-28

    The diterpene steviol glycoside, rebaudioside A, is a natural high potency non-caloric sweetener extracted from the leaves of Stevia rebaudiana. This compound shows a parabolic change in sweet taste intensity with temperature which contrasts with the general finding for other synthetic or natural sweeteners whose sweet taste increases with temperature. The nonmonotonic taste behavior was determined by sensory analysis using large taste panels. The conformational landscape of rebaudioside A was established at a range of temperatures by means of nuclear magnetic resonance and molecular dynamics simulation. The relationship between various conformations and the observed sweetness of rebaudioside A is described.

  19. Stevioside acts directly on pancreatic beta cells to secrete insulin: actions independent of cyclic adenosine monophosphate and adenosine triphosphate-sensitive K+-channel activity.

    Science.gov (United States)

    Jeppesen, P B; Gregersen, S; Poulsen, C R; Hermansen, K

    2000-02-01

    The natural sweetener stevioside, which is found in the plant Stevia rebaudiana Bertoni, has been used for many years in the treatment of diabetes among Indians in Paraguay and Brazil. However, the mechanism for the blood glucose-lowering effect remains unknown. To elucidate the impact of stevioside and its aglucon steviol on insulin release from normal mouse islets and the beta-cell line INS-1 were used. Both stevioside and steviol (1 nmol/L to 1 mmol/L) dose-dependently enhanced insulin secretion from incubated mouse islets in the presence of 16.7 mmol/L glucose (P diabetes mellitus.

  20. Effect of Rebaudioside A, a diterpenoid on glucose homeostasis in STZ-induced diabetic rats.

    Science.gov (United States)

    Saravanan, Ramalingam; Vengatash babu, Kaliyappan; Ramachandran, Vinayagam

    2012-09-01

    Rebaudioside A (Reb A), a major constituent of Stevia rebaudiana, was recently proposed as an insulinotropic agent. The aim of this investigation was to evaluate the antihyperglycemic effect of Reb A on the activities of hepatic enzymes of carbohydrate metabolism in streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in adult male Albino Wistar rats, weighing 180-200 g, by a single intraperitoneal injection at a dose of STZ (40 mg/kg body weight). Diabetic rats showed significant (Pdiabetic rats significantly (Pdiabetic rats. Thus, the results show that Reb A possesses an antihyperglycemic activity and provide evidence for its traditional usage in the control of diabetes.

  1. On the connection between nonmonotonic taste behavior and molecular conformation in solution: The case of rebaudioside-A

    Science.gov (United States)

    Chopade, Prashant D.; Sarma, Bipul; Santiso, Erik E.; Simpson, Jeffrey; Fry, John C.; Yurttas, Nese; Biermann, Kari L.; Chen, Jie; Trout, Bernhardt L.; Myerson, Allan S.

    2015-12-01

    The diterpene steviol glycoside, rebaudioside A, is a natural high potency non-caloric sweetener extracted from the leaves of Stevia rebaudiana. This compound shows a parabolic change in sweet taste intensity with temperature which contrasts with the general finding for other synthetic or natural sweeteners whose sweet taste increases with temperature. The nonmonotonic taste behavior was determined by sensory analysis using large taste panels. The conformational landscape of rebaudioside A was established at a range of temperatures by means of nuclear magnetic resonance and molecular dynamics simulation. The relationship between various conformations and the observed sweetness of rebaudioside A is described.

  2. Modelo para el desarrollo de la cadena agroindustrial de edulcolorantes alternativos

    OpenAIRE

    Villagomes Díaz, Tania Valentina

    2007-01-01

    94 hojas : ilustraciones, 29 x 21 cm + CD-ROM 1071 Basando la propuesta en el modelo de Porter de las 5 fuerzas, se ha planteado factores que determinarían el desarrollo y desenvolvimiento de una cadena de valor agro productiva para edulcorantes alternativos y en este caso específico para la Stevia rebaudiana, en las diferentes actividades principales del clúster: Producción Primaria, Producción Secundaria, Comercialización y Consumidor. Estos factores, los cuales según el modelo de Port...

  3. Steviamine, a new class of indolizidine alkaloid [(1R,2S,3R,5R,8aR-3-hydroxymethyl-5-methyloctahydroindolizine-1,2-diol hydrobromide

    Directory of Open Access Journals (Sweden)

    Amber L. Thompson

    2009-11-01

    Full Text Available X-ray crystallographic analysis of the title hydrobromide salt, C10H20N+·Br−, of (1R,2S,3R,5R,8aR-3-hydroxymethyl-5-methyloctahydroindolizine-1,2-diol defines the absolute and relative stereochemistry at the five chiral centres in steviamine, a new class of polyhydroxylated indolizidine alkaloid isolated from Stevia rebaudiana (Asteraceae leaves. In the crystal structure, molecules are linked by intermolecular O—H...Br and N—H...Br hydrogen bonds, forming double chains around the twofold screw axes along the b-axis direction. Intramolecular O—H...O interactions occur.

  4. In vitro conservation of twenty-three overexploited medicinal plants belonging to the Indian sub continent.

    Science.gov (United States)

    Verma, Priyanka; Mathur, Ajay Kumar; Jain, Sheetal Prasad; Mathur, Archana

    2012-01-01

    Twenty-three pharmaceutically important plants, namely, Elaeocarpus spharicus, Rheum emodi, Indigofera tinctoria, Picrorrhiza kurroa, Bergenia ciliata, Lavandula officinalis, Valeriana wallichii, Coleus forskohlii, Gentiana kurroo, Saussurea lappa, Stevia rebaudiana, Acorus calamus, Pyrethrum cinerariaefolium, Aloe vera, Bacopa monnieri, Salvia sclarea, Glycyrrhiza glabra, Swertia cordata, Psoralea corylifolia, Jurinea mollis, Ocimum sanctum, Paris polyphylla, and Papaver somniferum, which are at the verge of being endangered due to their overexploitation and collection from the wild, were successfully established in vitro. Collections were made from the different biodiversity zones of India including Western Himalaya, Northeast Himalaya, Gangetic plain, Western Ghats, Semiarid Zone, and Central Highlands. Aseptic cultures were raised at the morphogenic level of callus, suspension, axillary shoot, multiple shoot, and rooted plants. Synseeds were also produced from highly proliferating shoot cultures of Bacopa monnieri, Glycyrrhiza glabra, Stevia rebaudiana, Valeriana wallichii, Gentiana kurroo, Lavandula officinalis, and Papaver somniferum. In vitro flowering was observed in Papaver somniferum, Psoralea corylifolia, and Ocimum sanctum shoots cultures. Out of 23 plants, 18 plants were successfully hardened under glasshouse conditions.

  5. In Vitro Conservation of Twenty-Three Overexploited Medicinal Plants Belonging to the Indian Sub Continent

    Directory of Open Access Journals (Sweden)

    Priyanka Verma

    2012-01-01

    Full Text Available Twenty-three pharmaceutically important plants, namely, Elaeocarpus spharicus, Rheum emodi, Indigofera tinctoria, Picrorrhiza kurroa, Bergenia ciliata, Lavandula officinalis, Valeriana wallichii, Coleus forskohlii, Gentiana kurroo, Saussurea lappa, Stevia rebaudiana, Acorus calamus, Pyrethrum cinerariaefolium, Aloe vera, Bacopa monnieri, Salvia sclarea, Glycyrrhiza glabra, Swertia cordata, Psoralea corylifolia, Jurinea mollis, Ocimum sanctum, Paris polyphylla, and Papaver somniferum, which are at the verge of being endangered due to their overexploitation and collection from the wild, were successfully established in vitro. Collections were made from the different biodiversity zones of India including Western Himalaya, Northeast Himalaya, Gangetic plain, Western Ghats, Semiarid Zone, and Central Highlands. Aseptic cultures were raised at the morphogenic level of callus, suspension, axillary shoot, multiple shoot, and rooted plants. Synseeds were also produced from highly proliferating shoot cultures of Bacopa monnieri, Glycyrrhiza glabra, Stevia rebaudiana, Valeriana wallichii, Gentiana kurroo, Lavandula officinalis, and Papaver somniferum. In vitro flowering was observed in Papaver somniferum, Psoralea corylifolia, and Ocimum sanctum shoots cultures. Out of 23 plants, 18 plants were successfully hardened under glasshouse conditions.

  6. PECULIARITIES OF SECONDARY METABOLITES BIOSYNTHESIS IN PLANT CELL CULTURES

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    A.M. NOSOV

    2014-06-01

    Full Text Available metabolites formation in plant cell cultures of Panax spp., (ginsenosides; Dioscorea deltoidea (steroid glycosides; Ajuga reptans, Serratula coronata, Rhaponticum carthamoides (ecdisteroids; Polyscias spp., (triterpene glycosides, Taxus spp. (taxoids, Stevia rebaudiana (diterpene steviol-glycosides, Stephania glabra (alkaloids. They are some regular trends of secondary metabolites synthesis in the plant cell culture:It can be noted the stable synthesis of the compound promoting cell proliferation. Indeed, cell cultures of Dioscorea deltoidea were demonstrated to accumulate only furostanol glycosides, which promoted cell division. Furostanol glycoside content of Dioscorea strain DM-0.5 was up to 6 - 12% by dry biomass.Panax ginseng and P. japonicus plant cell cultures synthesize as minimum seven triterpene glycosides (ginsenosides, the productivity of these compounds was up to 6.0 - 8.0% on dry biomass.By contrast, the detectable synthesis of diterpene steviol-glycosides in cultivated cells of Stevia rebaudiana initiated in the mixotrophic cultures during chloroplast formation only.Despite these differences, or mainly due to them, plant cell cultures have become an attractive source of phytochemicals in alternative to collecting wild plants. It provides a guideline to bioreactor-based production of isoprenoids using undifferentiated plant cell cultures. 

  7. Estudio estereoquímico y conformacional de algunos metabolitos secundarios aislados de stevia pilosa y stevia tomentosa

    OpenAIRE

    Alvarez García, Rocio

    2013-01-01

    Un producto natural es todo compuesto de origen orgánico o inorgánico, que se halle en la naturaleza y que pueda ser procesado por el hombre.1 Generalmente, en el área de química orgánica, se consideran como productos naturales a aquellos provenientes principalmente de plantas, hongos, bacterias y organismos marinos, los cuales llevan a cabo dos tipos de metabolismo, el primario y el secundario.

  8. Biotechnological production of natural zero-calorie sweeteners.

    Science.gov (United States)

    Philippe, Ryan N; De Mey, Marjan; Anderson, Jeff; Ajikumar, Parayil Kumaran

    2014-04-01

    The increasing public awareness of adverse health impacts from excessive sugar consumption has created increasing interest in plant-derived, natural low-calorie or zero-calorie sweeteners. Two plant species which contain natural sweeteners, Stevia rebaudiana and Siraitia grosvenorii, have been extensively profiled to identify molecules with high intensity sweetening properties. However, sweetening ability does not necessarily make a product viable for commercial applications. Some criteria for product success are proposed to identify which targets are likely to be accepted by consumers. Limitations of plant-based production are discussed, and a case is put forward for the necessity of biotechnological production methods such as plant cell culture or microbial fermentation to meet needs for commercial-scale production of natural sweeteners. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Antihyperglycemic effects of stevioside in type 2 diabetic subjects

    DEFF Research Database (Denmark)

    Gregersen, Søren; Jeppesen, Per B; Holst, Jens Juul

    2004-01-01

    Stevioside is present in the plant Stevia rebaudiana Bertoni (SrB). Extracts of SrB have been used for the treatment of diabetes in, for example, Brazil, although a positive effect on glucose metabolism has not been unequivocally demonstrated. We studied the acute effects of stevioside in type 2...... diabetic patients. We hypothesize that supplementation with stevioside to a test meal causes a reduction in postprandial blood glucose. Twelve type 2 diabetic patients were included in an acute, paired cross-over study. A standard test meal was supplemented with either 1 g of stevioside or 1 g of maize...... starch (control). Blood samples were drawn at 30 minutes before and for 240 minutes after ingestion of the test meal. Compared to control, stevioside reduced the incremental area under the glucose response curve by 18% (P =.013). The insulinogenic index (AUC(i,insulin)/AUC(i,glucose)) was increased...

  10. Insight into anti-diabetic effect of low dose of stevioside.

    Science.gov (United States)

    Ilić, Vladimirka; Vukmirović, Saša; Stilinović, Nebojša; Čapo, Ivan; Arsenović, Milan; Milijašević, Boris

    2017-06-01

    Diabetes mellitus is a chronic disease characterized by abnormal carbohydrate, lipid and protein metabolism due to a lack of insulin or reduced target cell sensitivity to insulin. Stevia rebaudiana is an important source of biochemically active substances with proven anti-diabetic effect. The aim of this study was to determine anti-diabetic effects of the low dose of stevioside in NMRI Haan mice. Aqueous stevioside solution (20mg/kg body weight) was administered by oral route of administration. Anti-diabetic effect of stevioside was estimated by oral glucose tolerance test, adrenaline test after a 10day stevioside treatment, and alloxan induced hyperglycaemia in mice (two experimental groups, 10day stevioside treatment before and after alloxan administration). Aqueous stevioside solution prevented significant increase in glycaemia in oral glucose tolerance test (9.22±1.13 to 9.85±1.32mmol/l, Pdiabetes. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  11. Steviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity.

    Science.gov (United States)

    Philippaert, Koenraad; Pironet, Andy; Mesuere, Margot; Sones, William; Vermeiren, Laura; Kerselaers, Sara; Pinto, Sílvia; Segal, Andrei; Antoine, Nancy; Gysemans, Conny; Laureys, Jos; Lemaire, Katleen; Gilon, Patrick; Cuypers, Eva; Tytgat, Jan; Mathieu, Chantal; Schuit, Frans; Rorsman, Patrik; Talavera, Karel; Voets, Thomas; Vennekens, Rudi

    2017-03-31

    Steviol glycosides (SGs), such as stevioside and rebaudioside A, are natural, non-caloric sweet-tasting organic molecules, present in extracts of the scrub plant Stevia rebaudiana, which are widely used as sweeteners in consumer foods and beverages. TRPM5 is a Ca2+-activated cation channel expressed in type II taste receptor cells and pancreatic β-cells. Here we show that stevioside, rebaudioside A and their aglycon steviol potentiate the activity of TRPM5. We find that SGs potentiate perception of bitter, sweet and umami taste, and enhance glucose-induced insulin secretion in a Trpm5-dependent manner. Daily consumption of stevioside prevents development of high-fat-diet-induced diabetic hyperglycaemia in wild-type mice, but not in Trpm5-/- mice. These results elucidate a molecular mechanism of action of SGs and identify TRPM5 as a potential target to prevent and treat type 2 diabetes.

  12. Antihyperglycemic and blood pressure-reducing effects of stevioside in the diabetic Goto-Kakizaki rat.

    Science.gov (United States)

    Jeppesen, P B; Gregersen, S; Rolfsen, S E D; Jepsen, M; Colombo, M; Agger, A; Xiao, J; Kruhøffer, M; Orntoft, T; Hermansen, K

    2003-03-01

    Stevioside, a glycoside present in the leaves of the plant, Stevia rebaudiana Bertoni (SrB), has acute insulinotropic effects in vitro. Its potential antihyperglycemic and blood pressure-lowering effects were examined in a long-term study in the type 2 diabetic Goto-Kakizaki (GK) rat. Rats were fed 0.025 g x kg(-1) x d(-1) of stevioside (purity > 99.6%) for 6 weeks. An intra-arterial catheter was inserted into the rats after 5 weeks, and conscious rats were subjected to arterial glucose tolerance test (2.0 g x kg(-1)) during week 6. Stevioside had an antihyperglycemic effect (incremental area under the glucose response curve [IAUC]): 985 +/- 20 (stevioside) versus 1,575 +/- 21 (control) mmol/L x 180 minutes, (P diabetes and the metabolic syndrome. Copyright 2003, Elsevier Science (USA). All rights reserved.

  13. Influência do rebaudiosídeo A na solubilidade e no sabor do esteviosídeo

    Directory of Open Access Journals (Sweden)

    GOTO Airton

    1998-01-01

    Full Text Available Dentre os edulcorantes presentes nas folhas da Stevia rebaudiana Bertoni destacam-se pela quantidade presente, o esteviosídeo e o rebaudiosídeo A. O esteviosídeo, em maior quantidade, tem sua aplicação industrial restrita em alguns segmentos, devido ao seu sabor amargo residual e sua baixa solubilidade. Avaliou-se a influência do rebaudiosídeo A na solubilidade e no sabor do esteviosídeo. Observou-se que um adoçante composto com no mínimo 12,5% de rebaudiosídeo A na massa total é solúvel em água e se mantém estável. O sabor amargo residual diminui a me dida que aumenta o teor de rebaudiosídeo A na mistura de adoçantes.

  14. Mieux comprendre l’action des protéines édulcorantes : Article dans magazine de l'industrie agroalimentaire diffusé sur Processalimentaire.com

    OpenAIRE

    Briand, Loïc

    2010-01-01

    La quête de nouveaux édulcorants ne tarit pas. Si le rébaudioside A, issu de la Stevia rebaudiana , et le néotame, ont occupé le devant de la scène ces derniers mois, ils ne doivent pas occulter le potentiel de la famille des protéines édulcorantes. Un projet franco-allemand récemment labellisé par le pôle de compétitivité Vitagora vise à mieux comprendre les phénomènes moléculaires qui sous-tendent la perception de la saccharine, une protéine édulcorante de synthèse, et, plus original, de la...

  15. Preventive effects of a soy-based diet supplemented with stevioside on the development of the metabolic syndrome and type 2 diabetes in Zucker diabetic fatty rats.

    Science.gov (United States)

    Dyrskog, Stig Eric Underbjerg; Jeppesen, Per Bendix; Colombo, Michele; Abudula, Reziwanggu; Hermansen, Kjeld

    2005-09-01

    The world witnesses an explosive increase in diabetes, demanding intensified prevention and treatment not least for the low-income population. The plant, Stevia rebaudiana Bertoni, has been used for the treatment of diabetes in traditional medicine. We have previously demonstrated that stevioside, a diterpene glycoside isolated from the plant Stevia rebaudiana Bertoni, possesses insulinotropic, glucagonostatic, antihyperglycemic, and blood pressure-lowering effects in animal studies. We have also found that a dietary supplement, Abalon, of soy protein, isoflavones, and cotyledon fiber has beneficial effects on cardiovascular risk markers in type 2 diabetes. The aim of this study was to investigate if the combination of stevioside and a dietary supplement of soy protein possesses beneficial qualities in the treatment of type 2 diabetes and the metabolic syndrome. We randomized male Zucker diabetic fatty rats into 4 groups and fed them the different test diets for 10 weeks: (A) standard carbohydrate-rich laboratory diet (chow), (B) chow+stevioside (0.03 g/kg body weight [BW] per day), (C) 50% soy (Abalon)+50% chow (adjusted for vitamins and minerals), and (D) 50% soy (Abalon)+50% chow+stevioside 0.03 g/kg BW per day. We measured plasma glucose, blood pressure, weight, and food intake once weekly. The animals were equipped with an intra-arterial catheter, and at week 10, the conscious rats underwent an intra-arterial glucose tolerance test (2.0 g/kg BW). Stevioside exerts beneficial effects in type 2 diabetic Zucker diabetic fatty rats, that is, lowers blood glucose (area under the glucose curve [AUC(30min)]: group A vs B, a 19% reduction; and group C vs D, a 12% reduction; Pdiabetic subjects is needed to verify these promising results in animal diabetes.

  16. Evaluation of the Antihyperglycemic Effect of Minor Steviol Glycosides in Normoglycemic and Induced-Diabetic Wistar Rats.

    Science.gov (United States)

    Aranda-González, Irma; Moguel-Ordóñez, Yolanda; Chel-Guerrero, Luis; Segura-Campos, Maira; Betancur-Ancona, David

    2016-09-01

    Steviol glycosides are a family of compounds found in Stevia rebaudiana Bertoni that are responsible for sweetness capacity. The antihyperglycemic effect of the two major steviol glycosides, Rebaudioside A and Stevioside, has been studied and it has been found that despite having the same common structure, only Stevioside exerts an antihyperglycemic effect. Although other steviol derivatives are found in smaller amounts (minor steviol glycosides) in S. rebaudiana, whether or not they possess antihyperglycemic activity has not been evaluated. The aim of this study was to evaluate the antihyperglycemic effect of minor steviol glycosides in normoglycemic and diabetic (streptozotocin/nicotinamide) Wistar rats. Rats were subjected to an intraperitoneal glucose tolerance test (IPGTT) both before and after chronic treatment (28 days). After 6 h of fasting, IPGTT was conducted in pentobarbital-anesthetized rats using 1 g/kg of glucose plus 20 mg/kg of the minor glycoside (Dulcoside A, Rebaudioside B, C, D, or Steviolbioside) or control treatment (distilled water, glibenclamide, or metformin); the blood of the tip of the tail was collected at time 0, 15, 30, 60, and 120 min.; and blood glucose was measured, and its net area under the curve (AUCnet) was calculated. After 28-day chronic oral administration, IPGTT was again performed. Differences were considered significant at P diabetic Wistar rats. Considering the dose tested, it is unlikely that these glycosides have an effect on glucose in diabetic or normoglycemic humans.

  17. Steviol, an aglycone of steviol glycoside sweeteners, interacts with the pregnane X (PXR) and aryl hydrocarbon (AHR) receptors in detoxification regulation.

    Science.gov (United States)

    Dusek, Jan; Carazo, Alejandro; Trejtnar, Frantisek; Hyrsova, Lucie; Holas, Ondřej; Smutny, Tomas; Micuda, Stanislav; Pavek, Petr

    2017-11-01

    Stevia rebaudiana Bertoni is a herb known for the high content of natural sweeteners in its leaves. Its main secondary metabolite stevioside is used as non-caloric sweetener. No information, however, is available on whether stevioside or steviol interact with drug-metabolizing enzymes and pose the potential risk of food-drug interactions. Similarly, data are lacking on the interactions of steviol and stevioside with key nuclear receptors controlling the expression of the main drug metabolizing enzymes. We studied the interactions of steviol and stevioside with the pregnane X (PXR), vitamin D (VDR), constitutive androstane (CAR), farnesoid X (FXR), glucocorticoid (GR) and aryl hydrocarbon (AHR) receptors, which control expression of genes of xenobiotic metabolism. In addition, the inhibitory activities of steviol and stevioside towards the major cytochrome P450 enzymes CYP3A4, CYP2C9, CYP2D6, CYP1A2 and CYP2B6 were evaluated in vitro. We found that steviol moderately activated the PXR and AHR, resulting in the induction of their target genes including CYP3A4 and CYP1A2 in primary human hepatocytes. A weak inhibition of CYP3A4 and CYP2C9 with steviol was also found. Our results provide mechanistic data indicating that stevioside and stevia sweeteners may have the potential to induce food-drug interactions, a finding that warrants future prospective clinical investigation. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Rebaudioside A potently stimulates insulin secretion from isolated mouse islets: studies on the dose-, glucose-, and calcium-dependency.

    Science.gov (United States)

    Abudula, Reziwanggu; Jeppesen, Per Bendix; Rolfsen, Stig Eric D; Xiao, Jianzhong; Hermansen, Kjeld

    2004-10-01

    Extracts of leaves of the plant Stevia rebaudiana Bertoni (SrB), have been used for many years in traditional treatment of diabetes in South America. Stevia leaves contain diterpene glycosides, stevioside and rebaudioside A being the most abundant. Recently, it was demonstrated that stevioside stimulates the insulin secretion both in vitro and in vivo. Subsequently, we wanted to elucidate the influence of rebaudioside A on the insulin release from mouse islets using static incubations, as well as perifusion experiments. Rebaudioside A (10(-16) to 10(-6) mol/L) dose-dependently stimulated the insulin secretion in the presence of 16.7 mmol/L glucose (P 6.6 mmol/L. The effect of rebaudioside A is critically dependent on the presence of extracellular Ca2+, ie, rebaudioside A-induced insulin stimulation at high glucose disappears in the absence of extracellular Ca2+. In conclusion, rebaudioside A possesses insulinotropic effects and may serve a potential role as treatment in type 2 diabetes mellitus.

  19. Response of Syngonium podophyllum L. ‘White Butterfly’ shoot cultures to alternative media additives and gelling agents, and flow cytometric analysis of regenerants

    Directory of Open Access Journals (Sweden)

    JAIME A. TEIXEIRA DA SILVA

    2015-05-01

    Full Text Available Abstract. Teixeira da Silva JA. 2015. Response of Syngonium podophyllum L. ‘White Butterfly’ shoot cultures to alternative media additives and gelling agents, and flow cytometric analysis of regenerants. Nusantara Bioscience 7: 26-32. Syngonium podophyllum L. (arrowhead vine is a popular leafy indoor pot plant whose tissue culture has been established, primarily through in vitro shoot culture, but several interesting aspects have not yet been explored. In this study, cv. ‘White Butterfly’ was used to investigate the response of shoot formation to alternative gelling agents and media additives. Gellan gum (Gelrite® at 2 g/L resulted in greater leaf production, plantlet fresh weight and higher chlorophyll content (SPAD value than all other gelling agents tested, including agar, Bacto agar, phytagel, oatmeal agar, potato dextrose agar, barley starch and corn starch, when on a basal Hyponex® (NPK = 6.5: 6: 19; 3 g/L medium. Several alternative liquid medium additives tested (low and full fat milk, Coca-Cola®, coffee, Japanese green, Oolong and Darjeeling teas negatively impacted plant growth, stunted roots and decreased chlorophyll content (SPAD value of leaves. Plant growth on medium with refined sucrose or table sugar responded similarly. Poor growth was observed when crude extract from a high rebaudioside-containing stevia (Stevia rebaudiana Bertoni line - an artificial sweetener - was used. Leaf tissue from the control did not show any endopolyploidy but low levels of endopolyploidy (8C were detected in some treatments.

  20. LA INGENIERÍA GENÉTICA DE PLANTAS EN COLOMBIA: UN CAMINO EN CONSTRUCCIÓN

    Directory of Open Access Journals (Sweden)

    Alejandro Chaparro Giraldo

    2015-05-01

    Full Text Available Se reportan en Colombia trabajos en el desarrollo de líneas genéticamente modificadas (GM de arroz, yuca, algodón, papa, caña de azúcar, café, maíz, soya, estevia y crisantemo, por parte de centros centros de investigación (CIAT, CENICAÑA, CENICAFE, CIB, CORPOICA y tres universidades (Universidad Nacional de Colombia, Universidad Javeriana, Universidad de Antioquia. Para llegar a la liberación comercial de variedades GM, se requiere de verdaderas políticas públicas en ciencia y tecnología, que den financiación suficiente y oportuna, así como resolver problemas con la maraña de patentes que limitan o impiden el desarrollo de innovaciones biotecnológicas, además de un paquete regulatorio, que incrementa significativamente los costos de desarrollo.  ABSTRACT Here in Colombia have been reported efforts on the development of genetically modified (GM crops, like: rice, cassava, cotton, potato, sugar cane, coffee, corn, soy, stevia and chrysanthemum, by five research center (CIAT, CENICAÑA, CENICAFE, CIB, CORPOICA and three universities (Universidad Nacional de Colombia, Universidad Javeriana, Universidad de Antioquia. To get to the commercial release of GM varieties, real public policies on science and technology are needed, giving sufficient and timely funding, as well as to solve problems with the tangle of patents that limit or prevent the development of biotechnological innovations, and it is also required suffice funding to go through the regulatory issues, which significantly increase the cost of those developments.

  1. Biotechnological experiments in space flights on board of space stations

    Science.gov (United States)

    Nechitailo, Galina S.

    2012-07-01

    Space flight conditions are stressful for any plant and cause structural-functional transition due to mobiliation of adaptivity. In space flight experiments with pea tissue, wheat and arabidopsis we found anatomical-morphological transformations and biochemistry of plants. In following experiments, tissue of stevia (Stevia rebaudiana), potato (Solanum tuberosum), callus culture and culture and bulbs of suffron (Crocus sativus), callus culture of ginseng (Panax ginseng) were investigated. Experiments with stevia carried out in special chambers. The duration of experiment was 8-14 days. Board lamp was used for illumination of the plants. After experiment the plants grew in the same chamber and after 50 days the plants were moved into artificial ionexchange soil. The biochemical analysis of plants was done. The total concentration of glycozides and ratio of stevioside and rebauside were found different in space and ground plants. In following generations of stevia after flight the total concentration of stevioside and rebauside remains higher than in ground plants. Experiments with callus culture of suffron carried out in tubes. Duration of space flight experiment was 8-167 days. Board lamp was used for illumination of the plants. We found picrocitina pigment in the space plants but not in ground plants. Tissue culture of ginseng was grown in special container in thermostate under stable temperature of 22 ± 0,5 C. Duration of space experiment was from 8 to 167 days. Biological activity of space flight culutre was in 5 times higher than the ground culture. This difference was observed after recultivation of space flight samples on Earth during year after flight. Callus tissue of potato was grown in tubes in thermostate under stable temperature of 22 ± 0,5 C. Duration of space experiment was from 8 to 14 days. Concentration of regenerates in flight samples was in 5 times higher than in ground samples. The space flight experiments show, that microgravity and other

  2. Extemporaneous Formulations of Metformin for Pediatric Endocrinology: Physicochemical Integrity, Cytotoxicity of Sweeteners, and Quantitation of Plasma Levels.

    Science.gov (United States)

    Alemón-Medina, Radamés; Chávez-Pacheco, Juan Luis; Rivera-Espinosa, Liliana; Ramírez-Mendiola, Blanca; García-Álvarez, Raquel; Sámano-Salazar, Cynthia; Manuel Dávila-Borja, Víctor

    2015-08-01

    In response to the lack of pediatric formulations of metformin to control type 2 diabetes mellitus, hyperinsulinemic obesity, and dyslipidemias, we developed liquid formulations of metformin by dissolving 3 generic brands of 500-mg metformin(*,)(†,)(‡) tablets in water sweetened with sucralose. The physicochemical stabilities of these drugs were assessed and compared with those of formulations made with the innovative brand of metformin.(∥) A method to measure metformin plasma levels was proposed and then tested in 2 healthy subjects. This method may be useful to survey treatment compliance in the future. The biological safety profiles of the metformin solutions were assessed preliminarily in a system of hormone-dependent cancer cells (human breast cancer MCF-7 cells). Metformin solutions stored at 25°C exposed to light and at 25°C, 4°C, and 40°C protected from light, underwent physicochemical analysis by ultra-performance liquid chromatography with ultraviolet detection, the mobile phase consisting of 0.2 M potassium monobasic phosphate (pH 6.5), 4.6 mM sodium dodecyl sulphate (SDS), and acetonitrile (63:7:30) at a flow rate of 0.8 mL/min in a Symmetry C8 150 × 4.6 mm column (Milford, Massachusetts) at 40°C (236 nm). MCF-7 cells were grown in 96-well ELISA plates (2 × 10(5) cells/well) and were exposed to 10, 20, and 40 mg/mL sucralose(§), Stevia rebaudiana (Svetia; Metco, S.A. de C.V., México, D.F., Mexico), and metformin (50 mg/mL) for 48 hours. Cytotoxicity was determined using the WST-1 colorimetric assay (Roche, USA) in an Epoch ELISA reader (BioTek, Winooski, Vermont) at 440 nm. All brands of metformin were stable at all storage conditions for up to 30 days and retained >90% of the initial amount. Sucralose and Stevia rebaudiana caused zero cytotoxicity (ANOVA, P ≤ 0.05). The ultra-performance liquid chromatography with ultraviolet detection method was adapted to determine metformin level in very small blood samples (40 μL), which was

  3. Longevidade pós-colheita de alpínia [Alpinia purpurata (Vieill. K. Schum.] tratada com soluções de sacarose e extratos aquosos naturais Postharvest longevity of red ginger [Alpinia purpurata (Vieill. K. Schum.] treated with sucrose solutions and natural water extracts

    Directory of Open Access Journals (Sweden)

    H.L.S. Sant'Anna

    2010-09-01

    Full Text Available A alpínia (Alpinia purpurata é uma planta tropical encontrada em diversas regiões do Brasil com excelente potencial para a comercialização como flor de corte. Entretanto, a senescência das hastes pela exaustão da sacarose é um dos principais fatores que afetam a durabilidade comercial, sendo suprimida através da adição de sacarose nas soluções de pulsing. O presente trabalho objetivou avaliar o efeito de extratos aquosos de estévia (Stevia rebaudiana, anis estrelado (Illicium verum e sacarose em solução de pulsing na manutenção da qualidade pós-colheita de inflorescências de alpínia. Hastes florais uniformizadas em tamanho foram submetidas à solução de pulsing, por 12 horas, nas concentrações de 0,5; 1,0; 2,0 e 3,0%, tendo água destilada como controle. A melhor qualidade comercial das inflorescências foi obtida quando as hastes foram colocadas em pulsing em extratos de anis estrelado e estévia 1% e em solução de sacarose 2%, não apresentando diferença estatística entre si. A longevidade total das hastes não foi influenciada pelos tratamentos. A solução de sacarose pode ser substituída pelos extratos das plantas medicinais estudadas, na conservação pós-colheita de inflorescências de alpínia.Red ginger (Alpinia purpurata is a tropical plant found in several Brazilian regions with excellent potential for commercialization as cut flower. However, the senescence of flower stems due to the exhaustion of sucrose is a major factor that affects commercial durability and is suppressed through addition of sucrose in pulsing solutions. The aim of this work was to evaluate the effect of water extracts from stevia (Stevia rebaudiana, star anise (Illicium verum and sucrose in pulsing solution on the postharvest quality maintenance of red ginger inflorescences. Flower stems of uniform size were subjected to the pulsing solution for 12h at 0.5, 1.0, 2.0 and 3.0% concentrations, with distilled water as control. The best

  4. Steviol glycosides in purified stevia leaf extract sharing the same metabolic fate.

    Science.gov (United States)

    Purkayastha, Sidd; Markosyan, Avetik; Prakash, Indra; Bhusari, Sachin; Pugh, George; Lynch, Barry; Roberts, Ashley

    2016-06-01

    The safety of steviol glycosides is based on data available on several individual steviol glycosides and on the terminal absorbed metabolite, steviol. Many more steviol glycosides have been identified, but are not yet included in regulatory assessments. Demonstration that these glycosides share the same metabolic fate would indicate applicability of the same regulatory paradigm. In vitro incubation assays with pooled human fecal homogenates, using rebaudiosides A, B, C, D, E, F and M, as well as steviolbioside and dulcoside A, at two concentrations over 24-48 h, were conducted to assess the metabolic fate of various steviol glycoside classes and to demonstrate that likely all steviol glycosides are metabolized to steviol. The data show that glycosidic side chains containing glucose, rhamnose, xylose, fructose and deoxy-glucose, including combinations of α(1-2), β-1, β(1-2), β(1-3), and β(1-6) linkages, were degraded to steviol mostly within 24 h. Given a common metabolite structure and a shared metabolic fate, safety data available for individual steviol glycosides can be used to support safety of purified steviol glycosides in general. Therefore, steviol glycosides specifications adopted by the regulatory authorities should include all steviol glycosides belonging to the five groups of steviol glycosides and a group acceptable daily intake established. Copyright © 2016 Elsevier Inc. All rights reserved.

  5. Teaching Chemistry about "Stevia"--A Case of Cooperative Curriculum Innovation within PROFILES in Germany

    Science.gov (United States)

    Stuckey, Marc; Lippel, Marianne; Eilks, Ingo

    2014-01-01

    PROFILES is a project of teacher education and curriculum innovation funded by the FP7-programme of the European Union. The aim of PROFILES is implementing innovative science teaching practices incorporating a societal perspective and compassing inquiry-based science learning. The University of Bremen, Germany, as one of the partners, combines…

  6. Post-launch monitoring of novel foods/ingredients (revised version) : Methodology applied to additive stevia

    NARCIS (Netherlands)

    Brosens M; Hendriksen M; Niekerk EM; Temme EHM; van Raaij JMA; P&V; V&Z

    2014-01-01

    Dit rapport is de herziene versie van het oorspronkelijke rapport (mei 2014). Op pagina 3 staat een erratum met uitleg over de wijzigingen

    Het RIVM heeft een methode ontwikkeld waarmee in kaart kan worden gebracht welke producten een bepaald ingrediënt bevatten, en zo ja in welke

  7. Antihyperglycemic effects of stevioside in type 2 diabetic subjects.

    Science.gov (United States)

    Gregersen, Søren; Jeppesen, Per B; Holst, Jens J; Hermansen, Kjeld

    2004-01-01

    Stevioside is present in the plant Stevia rebaudiana Bertoni (SrB). Extracts of SrB have been used for the treatment of diabetes in, for example, Brazil, although a positive effect on glucose metabolism has not been unequivocally demonstrated. We studied the acute effects of stevioside in type 2 diabetic patients. We hypothesize that supplementation with stevioside to a test meal causes a reduction in postprandial blood glucose. Twelve type 2 diabetic patients were included in an acute, paired cross-over study. A standard test meal was supplemented with either 1 g of stevioside or 1 g of maize starch (control). Blood samples were drawn at 30 minutes before and for 240 minutes after ingestion of the test meal. Compared to control, stevioside reduced the incremental area under the glucose response curve by 18% (P =.013). The insulinogenic index (AUC(i,insulin)/AUC(i,glucose)) was increased by approximately 40% by stevioside compared to control (P diabetic patients, indicating beneficial effects on the glucose metabolism. Stevioside may be advantageous in the treatment of type 2 diabetes.

  8. Stevioside induces antihyperglycaemic, insulinotropic and glucagonostatic effects in vivo: studies in the diabetic Goto-Kakizaki (GK) rats.

    Science.gov (United States)

    Jeppesen, P B; Gregersen, S; Alstrup, K K; Hermansen, K

    2002-01-01

    Extracts of leaves from the plant Stevia rebaudiana Bertoni have been used in the traditional treatment of diabetes in Paraguay and Brazil. Recently, we demonstrated a direct insulinotropic effect in isolated mouse islets and the clonal beta cell line INS-1 of the glycoside stevioside that is present in large quantity in these leaves. Type 2 diabetes is a chronic metabolic disorder that results from defects in both insulin and glucagon secretion as well as insulin action. In the present study we wanted to unravel if stevioside in vivo exerts an antihyperglycaemic effect in a nonobese animal model of type 2 diabetes. An i.v. glucose tolerance test (IVGT) was carried out with and without stevioside in the type 2 diabetic Goto-Kakizaki (GK) rat, as well as in the normal Wistar rat. Stevioside (0.2 g/kg BW) and D-glucose (2.0 g/kg BW) were administered as i.v. bolus injections in anaesthetized rats. Stevioside significantly suppressed the glucose response to the IVGT in GK rats (incremental area under the curve (IAUC): 648 +/- 50 (stevioside) vs 958 +/- 85 mM x 120 min (control); P diabetic GK rat, and may have the potential of becoming a new antidiabetic drug for use in type 2 diabetes.

  9. Stevioside counteracts the glyburide-induced desensitization of the pancreatic beta-cell function in mice: studies in vitro.

    Science.gov (United States)

    Chen, Jianguo; Jeppesen, Per Bendix; Nordentoft, Iver; Hermansen, Kjeld

    2006-12-01

    The sulfonylurea glyburide (GB) is one of the most frequently used drugs in diabetes treatment. Long-term pretreatment with GB causes elevated basal insulin secretion (BIS) and decreased glucose-stimulated insulin secretion (GSIS). These characteristics may play an important role for the development of hypoglycemia and secondary failure. Stevioside (SVS), a substance extracted from leaves of Stevia rebaudiana Bertoni, enhances GSIS but not BIS. The aim of the present study was to clarify whether 24-hour exposure of isolated mouse islets to GB causes dose-dependent decrease in the GSIS and whether it is possible to counteract this desensitization by SVS. We also tested the impact of the incretin glucagon-like peptide-1 (GLP-1) on the GB-induced desensitization. After 24-hour preincubation with GB in combination with SVS or GLP-1, we measured the basal and glucose-stimulated insulin responses and the total islet insulin content. We also determined the fold change in gene expression of pancreatic and duodenal homeobox 1 and glucose transporter isoform 2. After 24-hour preincubation in 11.1 mmol/L glucose, GB (10(-11)-10(-3) mol/L) caused a dose-dependent decrease in GSIS (16.7 mmol/L glucose) (P diabetes mellitus.

  10. Apparent lack of pharmacological effect of steviol glycosides used as sweeteners in humans. A pilot study of repeated exposures in some normotensive and hypotensive individuals and in Type 1 and Type 2 diabetics.

    Science.gov (United States)

    Barriocanal, Luis A; Palacios, Mafalda; Benitez, Gilda; Benitez, Sussam; Jimenez, Jorge T; Jimenez, Nora; Rojas, Vicenta

    2008-06-01

    Steviol glycosides, isolated from the plant Stevia rebaudiana (Bertoni) Bertoni, have been used as safe sweetening agents for more than 30 years. Beneficial effects of high doses of steviol glycosides on hyperglycemia and hypertension have been previously described when these abnormalities are present. This study was designed to evaluate the effects of steviol glycosides on blood glucose and on blood pressure (BP) in 3 groups of individuals. This was a randomized, double-blind, placebo-controlled, long-term study in three groups of patients: Group 1: subjects with Type 1 diabetes; Group 2: subjects with Type 2 diabetes; and Group 3: subjects without diabetes and with normal/low-normal BP levels. The subjects in each group were randomly allocated to active treatment (the steviol glycoside stevioside: 250mg t.d.s.) or to placebo treatment and followed-up for 3 months. Post-treatment systolic BP, diastolic BP, glucose and glycated hemoglobin (HbA1c) were not significantly different from baseline measurements, except for the placebo Type 1 diabetics group where a significant difference was observed for systolic BP and glucose. No side effects were observed in the two treatment groups. This study shows that oral steviol glycosides, taken as sweetener are well tolerated and have no pharmacological effect.

  11. Antidiabetic potential of bioactive molecules coated chitosan nanoparticles in experimental rats.

    Science.gov (United States)

    Perumal, Venkatachalam; Manickam, Thiyagarajan; Bang, Keuk-Soo; Velmurugan, Palanivel; Oh, Byung-Taek

    2016-11-01

    The present study was carried out to examine the antidiabetic effects of chitosan nanoparticles (CNPs) loaded with (Stevia rebaudiana leaf extract-SRLE) bioactive molecules in a rat model of streptozotocin (STZ) induced diabetes mellitus. Successful crosslinking of the bioactive molecules to the chitosan nanoparticles was confirmed by Fourier Transform Infrared Spectroscopy (FTIR). The colloidal characteristics of the synthesized nanoparticles were revealed by X-ray Diffraction (XRD) analysis. Morphological analysis by Transmission Electron Microscopy (TEM) revealed that thebioactive molecule-loaded CNPs were well-dispersed and spherical or polygonal in shape with an average size ofdiabetic rats showed a significant reduction in their mean fasting blood glucose level compared with the diabetic control group. The serum levels of various enzymes viz., serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatases (ALP), lipid peroxidation, and antioxidant such as catalase (CAT), reduced glutathione (GSH), and superoxide dismutase (SOD) in the SRLE CNP-treated group were closer to normal levels than those in the diabetic control group. Copyright © 2016 Elsevier B.V. All rights reserved.

  12. Rebaudioside A inhibits pentylenetetrazol-induced convulsions in rats

    Directory of Open Access Journals (Sweden)

    Yigit Uyanikgil

    2016-09-01

    Full Text Available The safety of patients with epilepsy consuming sweetening agents, which is becoming increasingly prevalent for various reasons, is a topic that should be emphasized as sensitively as it is for other diseases. Patients with epilepsy consume sweetening agents for different reasons such being diabetic or overweight. They can occasionally be exposed to sweetening agents unrestrainedly through consuming convenience food, primarily beverages. This study aimed to investigate the effects of rebaudioside A (Reb-A, which is a steviol glycoside produced from the herb Stevia rebaudiana (Bertoni, on epileptic seizures and convulsions induced by pentylenetetrazole (PTZ. Forty-eight male rats were used. Twenty-four rats were administered 35 mg/kg PTZ to trigger epileptiform activity; the remaining 24 rats were administered 70 mg/kg PTZ to trigger the convulsion model. The epileptiform activity was evaluated by spike percentage, whereas convulsion was evaluated by Racine's Convulsion Scale and the onset time of the first myoclonic jerk. Statistical analysis revealed a statistically significant decrease in the Racine's Convulsion Scale score and increase in the latency of first myoclonic jerk in a dose-dependent manner for the rat groups in which PTZ epilepsy had been induced and Reb-A had been administered. For the groups that were administered Reb-A, the spike decrease was apparent in a dose-dependent manner, based on the spike percentage calculation. These results indicated that Reb-A has positive effects on PTZ-induced convulsions.

  13. Inhibitory effect of stevioside on calcium influx to produce antihypertension.

    Science.gov (United States)

    Lee, C N; Wong, K L; Liu, J C; Chen, Y J; Cheng, J T; Chan, P

    2001-12-01

    Stevioside is a sweet-tasting glycoside occurring abundantly in the leaves of Stevia rebaudiana (Compositae). It has been used popularly in Japan and Brazil as a sugar substitute for decades. Previous study has shown that it lowered blood pressure in spontaneously hypertensive rats (SHRs) when administered intravenously. This study shows that intraperitoneal injection of stevioside 25 mg/kg also has antihypertensive effect in SHRs. In isolated aortic rings from normal rats, stevioside could dose-dependently relax the vasopressin-induced vasoconstriction in both the presence and absence of endothelium. However, stevioside had no effect on phenylephrine- and KCl-induced phasic vasoconstriction. In addition, stevioside lost its influence on vasopressin-induced vasoconstriction in Ca(2+)-free medium. The results indicate that stevioside caused vasorelaxation via an inhibition of Ca(2+) influx into the blood vessel. This phenomenon was further confirmed in cultured aortic smooth muscle cells (A7r5). Using 10(-5) M methylene blue for 15 min, stevioside could still relax 10(-8) M vasopressin-induced vasoconstriction in isolated rat aortic rings, showing that this vasorelaxation effect was not related to nitric oxide. The present data show that the vasorelexation effect of stevioside was mediated mainly through Ca(2+) influx inhibition.

  14. Analýza nápojů slazených extrakty stévie cukerné

    OpenAIRE

    Procházka, Václav

    2013-01-01

    Steviolglykosidy jsou přírodní sladivé látky Stévie sladké (Stevia rebaudiana Bertoni), které příznivě působí na lidské zdraví a jejich relativní sladivost je 300krát vyšší než sladivost sacharózy. Jsou proto využívány ke slazení komerčních výrobků. Vzhledem k jejich potenciálním vlastnostem je výhodné mít vhodnou metodu pro jejich sledování. Vysokoúčinná kapalinová chromatografie (HPLC) je založena na principu rozdělování analytu mezi dvě vzájemně nemísitelné fáze pomocí vysokotlakého čerpad...

  15. Reduction of the Oxidative Stress Status Using Steviol Glycosides in a Fish Model (Cyprinus carpio)

    Science.gov (United States)

    Sánchez-Aceves, Livier Mireya; Dublán-García, Octavio; López-Martínez, Leticia-Xochitl; Novoa-Luna, Karen Adriana; Galar-Martínez, Marcela; Hernández-Navarro, María Dolores

    2017-01-01

    Steviol glycosides are sweetening compounds from the Stevia rebaudiana Bertoni plant. This product is considered safe for human consumption and was approved as a food additive by the Food and Drugs Administration (FDA) and European Food Safety Authority (EFSA). Its effects on the ecosystem have not been studied in depth; therefore, it is necessary to carry out ecotoxicological studies in organisms such as Cyprinus carpio. The present study aimed to evaluate the antioxidant activity by SGs on diverse tissues in C. carpio using oxidative stress (OS) biomarkers. To test the antioxidant activity, carps were exposed to four systems: (1) SGs free control, (2) CCl4 0.5 mL/kg, (3) SGs 1 g/L, and (4) CCl4 0.5 mL/kg + SGs 1 g/L at 96 h. The following biomarkers were analyzed: lipoperoxidation (LPX), hydroperoxide content (HPC), and protein carbonyl content (PCC), as well as antioxidant activity of superoxide dismutase (SOD) and catalase (CAT). It was found that both (3 and 4) systems' exposure decreases LPX, CHP, PCC, SOD, and CAT with respect to the CCl4 system. The results of this study demonstrate that the concentrations of SGs used are not capable of generating oxidative stress and, on the contrary, would appear to induce an antioxidant effect. PMID:28691017

  16. Reduction of the Oxidative Stress Status Using Steviol Glycosides in a Fish Model (Cyprinus carpio

    Directory of Open Access Journals (Sweden)

    Livier Mireya Sánchez-Aceves

    2017-01-01

    Full Text Available Steviol glycosides are sweetening compounds from the Stevia rebaudiana Bertoni plant. This product is considered safe for human consumption and was approved as a food additive by the Food and Drugs Administration (FDA and European Food Safety Authority (EFSA. Its effects on the ecosystem have not been studied in depth; therefore, it is necessary to carry out ecotoxicological studies in organisms such as Cyprinus carpio. The present study aimed to evaluate the antioxidant activity by SGs on diverse tissues in C. carpio using oxidative stress (OS biomarkers. To test the antioxidant activity, carps were exposed to four systems: (1 SGs free control, (2 CCl4 0.5 mL/kg, (3 SGs 1 g/L, and (4 CCl4 0.5 mL/kg + SGs 1 g/L at 96 h. The following biomarkers were analyzed: lipoperoxidation (LPX, hydroperoxide content (HPC, and protein carbonyl content (PCC, as well as antioxidant activity of superoxide dismutase (SOD and catalase (CAT. It was found that both (3 and 4 systems’ exposure decreases LPX, CHP, PCC, SOD, and CAT with respect to the CCl4 system. The results of this study demonstrate that the concentrations of SGs used are not capable of generating oxidative stress and, on the contrary, would appear to induce an antioxidant effect.

  17. Production Optimization of Low-Calorie Orange Nectar Using Stevioside Sweetener and Evaluation of Its Physicochemical Properties during Storage

    Directory of Open Access Journals (Sweden)

    S. Hosseini

    2015-09-01

    Full Text Available Nowadays low-calorie products are increasingly becoming popular. One of the methods to produce low-calorie food is replacement of sugar (sucrose with low-calorie sweeteners such as stevioside. This compound is isolated from the leaves of the Paraguayan plant, Stevia rebaudiana Bertoni. Since orange juice is a popular beverage with an important role in human nutrition, production of low-calorie orange nectar (containing 60% natural juice and optimization of formulation parameters using response surface methodology (RSM was the purpose of this study. Three levels of independent variables, sugar, stevioside and pectin were used to optimize formulation and two responses of brix and viscosity were evaluated. After the determination of the best formula, they were produced and stored at refrigerator (4°C and ambient (25°C temperatures for 60 days and their physicochemical properties were measured in 20 days intervals. The results showed that after 60 days of storage, stevioside content was reduced (5%. Sucrose, turbidity and viscosity were reduced during storage but brix did not indicate a notable change over the course of the study. These changes were greater at the higher storage temperature (except brix. At the end of the storage, optimal treatment had higher turbidity and total phenolic contents than the blank sample. Results showed that it is possible to produce orange nectar with 70% decrease in its sugar content by using 0.06% of stevioside and 0.03% of pectin, without any significant effects on physicochemical and sensory properties.

  18. Estudios etnobotánicos en la conurbación Buenos Aires-La Plata, Argentina: Productos de plantas medicinales introducidos por inmigrantes paraguayos

    Directory of Open Access Journals (Sweden)

    Julio A. Hurrell

    2016-01-01

    Full Text Available Este trabajo incluye resultados parciales de una línea de investigación en Etnobotánica urbana, que estudia los conocimientos locales sobre las plantas, sus partes y productos derivados en la conurbación Buenos Aires-La Plata. Se incluyen productos de plantas medicinales de reciente ingreso en el área de estudio a través de inmigrantes oriundos de Paraguay. Los productos corresponden a 10 especies: Achyrocline alata(“jateí-ka’á”, Baccharis gaudichaudiana (“jaguareté-ka’á”, Cuphea lysimachioides(“ysypó-peré”, Euphorbia serpens(“tupasý-kambý”, Genipa americana(“ñandypá”, Helietta apiculata(“yvyra ovy”, Lycopodiella cernnua(“itápotý”, Rhynchosia balansae(“urusú-he’ě”, Stevia rebaudiana(“ka’á-he’ě” y Victoria cruziana(“jacaré-yrupé”. Para cada caso se indican sus productos, muestras de referencia, usos asignados, actividad biológica y efectos estudiados. La presencia de los productos de estas especies en el área metropolitana indica un aumento de la diversidad biocultural local, tanto de plantas y productos como de sus conocimientos asociados.

  19. Utilização de zeólitas modificadas CaX e MgX como adsorventes de compostos orgânicos

    Directory of Open Access Journals (Sweden)

    Denise Maria Malachini Miotto

    2002-04-01

    Full Text Available Neste trabalho, foi avaliada a capacidade de adsorção de zeólitas modificadas, CaX e MgX, em contato com uma solução do subproduto do processo de extração de esteviosídeo, um adoçante extraído das folhas de Stevia rebaudiana. Os efeitos do processo de adsorção na estrutura zeolítica foram avaliados por meio de diversas técnicas de caracterização. As análises térmicas mostraram que os compostos adsorvidos sofrem pequenas mudanças estruturais sob aquecimento. A partir da espectroscopia ao infravermelho, foi verificado que o processo de adsorção não modifica a estrutura da zeólita. As medidas de área superficial e volume de poros indicaram que a adsorção ocorre preferencialmente na superfície externa e nos mesoporos. Finalmente, os resultados de acidez indicaram que a adsorção dos compostos orgânicos coloridos ocorre principalmente nos cátions de compensação.

  20. Purificação do subproduto do processo de extração de esteviosídeo Purification of the by-product of the stevioside extraction process

    Directory of Open Access Journals (Sweden)

    Denise Maria Malachini Miotto

    2004-03-01

    Full Text Available O caramelo consiste no subproduto do processo de extração dos edulcorantes das folhas de Stevia rebaudiana Bertoni. Esse subproduto apresenta teores significativos dos edulcorantes esteviosídeo e rebaudiosídeo A não extraídos no processo e grande quantidade de compostos das folhas que lhe conferem, respectivamente, sabor doce e coloração escura. Desta forma, a retirada dos compostos das folhas presentes no caramelo torna possível seu reaproveitamento como edulcorante. Portanto, o caramelo foi purificado por meio de adsorção em zeólitas modificadas, CaX e MgX. Foram realizados dois experimentos: um teste de saturação dos adsorventes para avaliar sua capacidade adsortiva e um teste de máxima clarificação para determinar a máxima purificação alcançada por adsorção em zeólitas. Os resultados mostraram que CaX é o adsorvente mais eficaz. As zeólitas podem ser reutilizadas por até duas vezes, necessitando regeneração em seguida. O teste de máxima clarificação apresentou soluções quase límpidas, com altos níveis de clarificação (80-90% dos compostos com maior afinidade, mas elevadas retenções dos edulcorantes (~70%, pois as zeólitas conseguem reter grande parte dos pigmentos da solução de caramelo, arrastando também esteviosídeo e rebaudiosídeo A, com baixo rendimento de recuperação dos edulcorantes, mas considerado satisfatório tendo em vista que o caramelo, apesar de rico em edulcorantes, não tem aplicação atualmente.Caramel is the by-product of the sweeteners extraction process of Stevia rebaudiana Bertoni leaves. This by-product shows significant contents of the sweeteners stevioside and rebaudioside A not extracted in the process and large quantity of leaf components, that give it, respectively, sweet flavor and dark color. Like this, the retreat of the leaf components of the caramel makes possible its reuse as a sweetener. Therefore, the caramel was purified by adsorption in modified zeolites

  1. Evaluation of the antifungal activity by plant extracts against Colletotrichum gloeosporioides Penz Avaliação da atividade anti-fúngica de extratos vegetais contra Colletotrichum gloeosporioides Penz

    Directory of Open Access Journals (Sweden)

    Polyanna Alves Silva

    2008-04-01

    Full Text Available Aiming to develop more efficient and environmental friendly methods than those available to control Colletotrichum gloeosporioides Penz, which causes blister spot in coffee trees, a search for plants able to produce substances active against such pathogen was carried out. Thus, extracts of 48 plant species, collected at Alto Rio Grande region, in Minas Gerais, were prepared and submitted to in vitro assays with that fungus. The best results were obtained with the extracts prepared from Digitalis lanata Ehrh, Origanum manjorona L., Plantago lanceolata Hook. and Stevia rebaudiana (Bertoni Bertoni, which inhibited C. gloeosporioides spores germination. After dilution of some active extracts with aqueous 1 % Tween 80 solution in a 1:2 or 1:3 ratio (extract:aqueous solution, their antifungal activity vanished. Some of the active extracts were also submitted to freeze drying and none of them presented any alteration in their antifungal activity. Concluding, several plants presented potential to be used in the search for new bioactive substances to control C. gloeosporioides, especially O. manjorona L., which inhibited 96 % of the fungus spores germination.Com vistas a contribuir para o desenvolvimento de métodos mais eficientes e menos agressivos ao ambiente que aqueles disponíveis para o controle de Colletotrichum gloeosporioides Penz, causador da mancha manteigosa em cafeeiros, buscou-se identificar espécies vegetais produtoras de substâncias ativas contra o referido patógeno. Para tanto, prepararam-se extratos de 48 espécies vegetais, coletadas na região do Alto Rio Grande, em Minas Gerais, para serem submetidos a testes in vitro com o mencionado fungo. Constatou-se que os melhores resultados foram obtidos com os extratos oriundos de Digitalis lanata Ehrh, Origanum manjorona L., Plantago lanceolata Hook. e Stevia rebaudiana (Bertoni Bertoni que inibiram a germinação dos esporos de C. gloeosporioides. Alguns dos extratos ativos tamb

  2. The diterpene glycoside, rebaudioside A, does not improve glycemic control or affect blood pressure after eight weeks treatment in the Goto-Kakizaki rat.

    Science.gov (United States)

    Dyrskog, Stig E U; Jeppesen, Per B; Chen, Jianguo; Christensen, Lars P; Hermansen, Kjeld

    2005-01-01

    The plant, Stevia rebaudiana Bertoni (SrB), has been used for the treatment of diabetes in traditional medicine. Previously, we have demonstrated that long-term administration of the glycoside stevioside has insulinotropic, glucagonostatic, anti-hyperglycemic and blood pressure-lowering effects in type 2 diabetic animal models. The aim of this study was to elucidate if long-term administration of rebaudioside A, another glycoside isolated from the plant SrB, could improve glycemic control and lower blood pressure in an animal model of type 2 diabetes. We divided male Goto-Kakizaki (GK) rats into two groups which were fed a standard laboratory chow diet for eight weeks. The diet was supplemented with oral rebaudioside A (0.025 g/kg BW/day) in the experimental group. Blood glucose, weight, blood pressure and food intake were measured weekly. Animals were equipped with an intra-arterial catheter, and at week eight the conscious rats underwent an intra-arterial glucose tolerance test (IAGTT) (2.0 g/kg BW). During the IAGTT, the level of glucose, glucagon, and insulin responses did not differ significantly between the two groups. Fasting levels of glucose, glucagon, insulin or levels of blood lipids did not differ between the groups throughout the study period. We observed no effect on blood pressure or weight development. In conclusion, oral supplementation with rebaudioside A (0.025 g/kg BW/day) for eight weeks did not influence blood pressure or glycemic control in GK rats. Rebaudioside A failed to show the beneficial effects in diabetic animals previously demonstrated for stevioside.

  3. Diversion of carbon flux from gibberellin to steviol biosynthesis by over-expressing SrKA13H induced dwarfism and abnormality in pollen germination and seed set behaviour of transgenic Arabidopsis.

    Science.gov (United States)

    Guleria, Praveen; Masand, Shikha; Yadav, Sudesh Kumar

    2015-07-01

    This paper documents the engineering of Arabidopsis thaliana for the ectopic over-expression of SrKA13H (ent-kaurenoic acid-13 hydroxylase) cDNA from Stevia rebaudiana. HPLC analysis revealed the significant accumulation of steviol (1-3 μg g(-1) DW) in two independent transgenic Arabidopsis lines over-expressing SrKA13H compared with the control. Independent of the steviol concentrations detected, both transgenic lines showed similar reductions in endogenous bioactive gibberellins (GA1 and GA4). They possessed phenotypic similarity to gibberellin-deficient mutants. The reduction in endogenous gibberellin content was found to be responsible for dwarfism in the transgenics. The exogenous application of GA3 could rescue the transgenics from dwarfism. The hypocotyl, rosette area, and stem length were all considerably reduced in the transgenics. A noteworthy decrease in pollen viability was noticed and, similarly, a retardation of 60-80% in pollen germination rate was observed. The exogenous application of steviol (0.2, 0.5, and 1.0 μg ml(-1)) did not influence pollen germination efficiency. This has suggested that in planta formation of steviol was not responsible for the observed changes in transgenic Arabidopsis. Further, the seed yield of the transgenics was reduced by 24-48%. Hence, this study reports for the first time that over-expression of SrKA13H cDNA in Arabidopsis has diverted the gibberellin biosynthetic route towards steviol biosynthesis. The Arabidopsis transgenics showed a significant reduction in endogenous gibberellins that might be responsible for the dwarfism, and the abnormal behaviour of pollen germination and seed set. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  4. Genomics of Compositae crops: reference transcriptome assemblies and evidence of hybridization with wild relatives.

    Science.gov (United States)

    Hodgins, Kathryn A; Lai, Zhao; Oliveira, Luiz O; Still, David W; Scascitelli, Moira; Barker, Michael S; Kane, Nolan C; Dempewolf, Hannes; Kozik, Alex; Kesseli, Richard V; Burke, John M; Michelmore, Richard W; Rieseberg, Loren H

    2014-01-01

    Although the Compositae harbours only two major food crops, sunflower and lettuce, many other species in this family are utilized by humans and have experienced various levels of domestication. Here, we have used next-generation sequencing technology to develop 15 reference transcriptome assemblies for Compositae crops or their wild relatives. These data allow us to gain insight into the evolutionary and genomic consequences of plant domestication. Specifically, we performed Illumina sequencing of Cichorium endivia, Cichorium intybus, Echinacea angustifolia, Iva annua, Helianthus tuberosus, Dahlia hybrida, Leontodon taraxacoides and Glebionis segetum, as well 454 sequencing of Guizotia scabra, Stevia rebaudiana, Parthenium argentatum and Smallanthus sonchifolius. Illumina reads were assembled using Trinity, and 454 reads were assembled using MIRA and CAP3. We evaluated the coverage of the transcriptomes using BLASTX analysis of a set of ultra-conserved orthologs (UCOs) and recovered most of these genes (88-98%). We found a correlation between contig length and read length for the 454 assemblies, and greater contig lengths for the 454 compared with the Illumina assemblies. This suggests that longer reads can aid in the assembly of more complete transcripts. Finally, we compared the divergence of orthologs at synonymous sites (Ks) between Compositae crops and their wild relatives and found greater divergence when the progenitors were self-incompatible. We also found greater divergence between pairs of taxa that had some evidence of postzygotic isolation. For several more distantly related congeners, such as chicory and endive, we identified a signature of introgression in the distribution of Ks values. © 2013 John Wiley & Sons Ltd.

  5. Enhanced production of steviol glycosides in mycorrhizal plants: a concerted effect of arbuscular mycorrhizal symbiosis on transcription of biosynthetic genes.

    Science.gov (United States)

    Mandal, Shantanu; Upadhyay, Shivangi; Singh, Ved Pal; Kapoor, Rupam

    2015-04-01

    Stevia rebaudiana (Bertoni) produces steviol glycosides (SGs)--stevioside (stev) and rebaudioside-A (reb-A) that are valued as low calorie sweeteners. Inoculation with arbuscular mycorrhizal fungi (AMF) augments SGs production, though the effect of this interaction on SGs biosynthesis has not been studied at molecular level. In this study transcription profiles of eleven key genes grouped under three stages of the SGs biosynthesis pathway were compared. The transcript analysis showed upregulation of genes encoding 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway enzymes viz.,1-deoxy-D-xylulose 5-phospate synthase (DXS), 1-deoxy-D-xylulose 5-phospate reductoisomerase (DXR) and 2-C-methyl-D-erytrithol 2,4-cyclodiphosphate synthase (MDS) in mycorrhizal (M) plants. Zn and Mn are imperative for the expression of MDS and their enhanced uptake in M plants could be responsible for the increased transcription of MDS. Furthermore, in the second stage of SGs biosynthesis pathway, mycorrhization enhanced the transcription of copalyl diphosphate synthase (CPPS) and kaurenoic acid hydroxylase (KAH). Their expression is decisive for SGs biosynthesis as CPPS regulates flow of metabolites towards synthesis of kaurenoid precursors and KAH directs these towards steviol synthesis instead of gibberellins. In the third stage glucosylation of steviol to reb-A by four specific uridine diphosphate (UDP)-dependent glycosyltransferases (UGTs) occurs. While higher transcription of all the three characterized UGTs in M plants explains augmented production of SGs; higher transcript levels of UGT76G1, specifically improved reb-A to stev ratio implying increased sweetness. The work signifies that AM symbiosis upregulates the transcription of all eleven SGs biosynthesis genes as a result of improved nutrition and enhanced sugar concentration due to increased photosynthesis in M plants. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  6. Equivalência de dulçor e poder edulcorante de edulcorantes em função da temperatura de consumo em bebidas preparadas com chá-mate em pó solúvel Equi-sweetness and sweetening power of different sweetening agents in differents temperatures of consumption of tea drink in soluble power

    Directory of Open Access Journals (Sweden)

    Juliana Maria Porto Cardoso

    2004-09-01

    Full Text Available No presente estudo foi verificado o efeito da temperatura de consumo na equivalência de doçura e no poder edulcorante de diferentes agentes adoçantes em bebida de chá-mate em pó solúvel. Foram avaliados: aspartame, sucralose, mistura ciclamato/sacarina 2:1, Stevia e acessulfame-K, tendo como referência a sacarose. Todos os estudos foram realizados a 6±2ºC e a 45±2ºC. Primeiramente foi determinada a doçura ideal, utillizando-se escala do ideal com 30 provadores consumidores da bebida. Em seguida, foi determinada a doçura equivalente à sacarose (na doçura considerada ideal para cada edulcorante estudado, e seu poder edulcorante nas duas temperaturas de estudo. Para tal foi aplicado o método de estimação de magnitude, utilizando-se uma equipe de 10 provadores selecionados e treinados. A doçura ideal de sacarose foi de 8,3% para a bebida de chá-mate solúvel, sem diferença significativa entre as temperaturas de estudo. Ocorreram diferenças sensoriais importantes em função da temperatura, pois, enquanto para alguns edulcorantes o aumento de temperatura provocou diminuição na potência edulcorante, para outros foi observado aumento do poder edulcorante. Portanto, não se deve generalizar as alterações no poder edulcorante em função da temperatura, pois ela pode variar em função da classe química envolvida e do meio de dispersão em que se encontra.In this work the effect of temperature of consumption in the equi-sweetness and sweetening power of different sweetening agents was verified in tea drink in soluble powder. The panelists had evaluated: aspartame, sucralose, mixture of cyclamate/saccharin (2:1, Stevia [Stevia rebaudiana (Bert. Bertoni] and acesulfame-K as sweeteners, having sucrose as reference. All sensory tests were carried-out at 6±2ºC and 45±2ºC. Firstly the ideal sweetness was determined by just-about-right scale with 30 consumers of tea drink. After the ideal sweetness of sucrose determination, the

  7. Plants collected by Th. Herzog on his second Bolivian journey, 1910—1911. Part VIII. Compositae

    NARCIS (Netherlands)

    Koster, Joséphine Th.

    1947-01-01

    After a paper had been published i.a. on the species of Stevia of the collection mentioned in the heading¹) another Stevia from the same collection came into the author’s hands. It proved to be new. The American genus Stevia has been treated in local revisions by B. L. Robinson (in Gray Herb.

  8. Characteristics of non caloric dulcorants and their use in children

    Directory of Open Access Journals (Sweden)

    Calzada León Raúl

    2014-07-01

    Full Text Available This is a descriptive review of the elaboration routes, the physical and chemical characteristics, metabolism and clearance, sweetener level, residual flavor, maximal recommended ingestion, security levels and the assessment of growth and development problems in children (from newborns, including prematures, to the end of puberty, of the main no caloric edulcorants used in Mexico. The no caloric edulcorants included in this review are: aspartame, acesulfame-K, sucralose, sacarin, ciclamates, thaumatin, D- tagatose, estevia and alitame.

  9. Neurotropic effects of aspartame, stevia and sucralose on memory retention and on the histology of the hippocampus of the ICR mice(Mus musculus)

    National Research Council Canada - National Science Library

    Lejan Miguel Alabastro Villareal Rachelle Anne Montes Cruz Michael Bagui Ples Rodel Jonathan Santos Vitor

    2016-01-01

    ...: In this study, 20 mice were used to determine if there is an effect of consuming the maximum allowable dose of the non-nutritive sweeteners on the memory retention and on the histology of the hippocampus...

  10. In vitro bioassay investigations of the endocrine disrupting potential of steviol glycosides and their metabolite steviol, components of the natural sweetener Stevia

    DEFF Research Database (Denmark)

    Shannon, Maeve; Rehfeld, Anders; Frizzell, Caroline

    2016-01-01

    a steroidal structure and therefore may have the potential to act as an endocrine disruptor in the body. Reporter gene assays (RGAs), H295R steroidogenesis assay and Ca(2+) fluorimetry based assays using human sperm cells have been used to assess the endocrine disrupting potential of two steviol glycosides...... of sperm, causing a rapid influx of Ca(2+). The response was fully inhibited using a specific CatSper inhibitor. These findings highlight the potential for steviol to act as a potential endocrine disruptor....

  11. Plantas medicinales empleadas por pacientes diabéticos en Paraguay

    Directory of Open Access Journals (Sweden)

    Mabel MAIDANA

    2015-01-01

    Full Text Available Un gran porcentaje de la población mundial, utiliza plantas medicinales para hacer frente a sus necesidades de salud. En Paraguay se utiliza un gran número de especies vegetales con fines medicinales, para diferentes afecciones, entre ellas la diabetes. El presente trabajo tuvo como objetivo determinar las plantas medicinales empleadas por pacientes que consultan el Programa Nacional de Diabetes, Centro de Salud Nº 9, de Asunción, que forma parte de un programa de Atención Farmacéutica, desarrollado dentro del marco de la Tesis Doctoral: “Evaluación de un programa de Atención Farmacéutica en paceintes con diabetes mellitus tipo 2”. Se realizaron encuestas a 61 pacientes con diabetes mellitus tipo 2, que consultaron en el mencionado programa, entre octubre y diciembre del año 2011. De los 61 pacientes entrevistados, 17 eran hombres y 44 mujeres, la edad promedio fue de 55 años ± 10,6 años, el 45,9% vive en Asunción y 54,1% en diferentes ciudades del Departamento Central. El 78,1% utiliza plantas medicinales sin prescripción médica, el 18,8% no las utiliza y el 3,1% no contestó la pregunta. Los pacientes mencionaron 33 especies vegetales empleadas con fines medicinales. Las mismas se encuentran distribuidas en 25 familias, siendo la Asteraceae la familia mayormente representada con el 21,6%; la parte más utilizada es la hoja, (48,5%, la forma de empleo más reportada fue el tereré, bebida tradicional de Paraguay semejante al mate (infusión de yerba mate, pero frío; con el 51,5%. Las 5 especies más reportadas fueron: Moringa oleífera (Fabaceae; Artemisia absinthium, Tithonia diversifolia, Baccharis trimera y Stevia rebaudiana todas de la familia Asteraceae. Los pacientes con diabetes mellitus tipo 2 que emplean plantas medicinales, lo hacen de forma concomitante con medicamentos de síntesis, de los cuales el 47% son para el sistema cardiovascular, el 26% son antidiabéticos y el 17% para el sistema nervioso.

  12. Increase of insulin sensitivity by stevioside in fructose-rich chow-fed rats.

    Science.gov (United States)

    Chang, J-C; Wu, M C; Liu, I-M; Cheng, J-T

    2005-10-01

    The intake of dietary fructose has undergone a marked increase around the world, especially the developed countries, in recent times. Stevioside, a glycoside contained in the leaves of Stevia rebaudiana Bertoni (Compositae), was used to screen the effect induced by a diet containing 60% fructose on insulin resistance in rats. Single oral administration of stevioside for 90 min decreased plasma glucose concentrations in a dose-dependent manner in rats receiving fructose-rich chow for four weeks. In addition, insulin action on glucose disposal rate was measured using the glucose-insulin index, the product of the areas under the curve of glucose, and insulin during the intraperitoneal glucose tolerance test. Oral administration of stevioside (5.0 mg/kg) in rats given four weeks of fructose-rich chow for 90 min reversed the value of glucose-insulin index, indicating that stevioside has the ability to improve insulin sensitivity in this insulin-resistant animal model. Time for the loss of plasma glucose lowering response to tolbutamide (10.0 mg/kg, i. p.) in fructose-rich chow fed rats was also markedly delayed by repeated stevioside treatment three times daily compared to the vehicle-treated group. The plasma glucose-lowering activity of tolbutamide was introduced to account for varying levels of endogenous insulin secretion, and is widely used as the indicator of insulin resistance development. Thus, it provided the supportive data that repeated oral administration of stevioside delayed the development of insulin resistance in rats on a high-fructose diet. Increased insulin sensitivity by stevioside administration was further identified using the plasma glucose-lowering action of exogenous insulin in streptozotocin-induced diabetic rats (STZ-diabetic rats). Oral administration of stevioside at 0.2 mg/kg three times daily into STZ-diabetic rats for ten days increased the response to exogenous insulin. Taken together, this demonstrated that oral administration of

  13. Molecular evidence of insulinomimetic property exhibited by steviol and stevioside in diabetes induced L6 and 3T3L1 cells.

    Science.gov (United States)

    Bhasker, Salini; Madhav, Harish; Chinnamma, Mohankumar

    2015-10-15

    The defective responsiveness of body tissues to insulin involves the insulin receptors of cell membranes. The binding of insulin to its receptor induce an increase of high affinity glucose transporter molecules in target cell surface that enhance the uptake of glucose in to these cells. The WHO expert committee recommended the importance to investigate the hypoglycemic agents from plant origin, which are used in traditional medicine for the treatment of diabetes. Stevioside, a natural sweetener and a diterpene glycoside extracted from Stevia rebaudiana (Bertoni) has been used as an anti-hyperglycemic agent for the treatment of diabetes for decades. To reveal the molecular mechanism underlying the insulinomimetic activity of stevioside and its aglycone metabolite, steviol using cell line models. Efficacy of stevioside and steviol in inducing glucose absorption was studied at transcript level, protein level and by measuring glucose absorption in the cell using in-vitro cell line studies. Quantification of glucose transporter (GLUT4) transcript was done in 3T3-L1 adipocytes and L6 myotubes by qPCR using RPL23 as the internal control. GLUT4 protein was quantified using anti GLUT4 antibody by ELISA and radioactive glucose uptake studies were done to measure the rate of glucose absorption. The absolute and relative quantitation of GLUT4 gene by qPCR showed the activation of GLUT4 transcript at lower concentration of steviol (1 µM) and higher concentration of stevioside (100 µM) in both L6 myotubes and 3T3-L1 adipocytes. The increased level of glut4 protein and the glucose uptake in both the cell lines using the same concentration of steviol and stevioside further supports the qPCR data. The copy number and the expression level of GLUT4 gene, the amount of GLUT4 protein and the glucose uptake efficacy support the insulinomimetic effect of steviol and stevioside. The results of the study clearly demonstrate the functional similarity of steviol and stevioside with that of

  14. Soil wind erosion in ecological olive trees in the Tabernas desert (southeastern Spain): a wind tunnel experiment

    Science.gov (United States)

    Asensio, Carlos; Lozano, Francisco Javier; Gallardo, Pedro; Giménez, Antonio

    2016-08-01

    -erodible fraction aggregation of CaCO3, a Stevia rebaudiana cover crop was planted between the rows in this soil type and this favored retention of particles in vegetation.

  15. Uso de edulcorante como palatabilizante na dieta de leitões desmamados

    Directory of Open Access Journals (Sweden)

    Fabio Enrique Lemos Budiño

    2014-01-01

    Full Text Available Objetivou-se determinar os efeitos da adição de um edulcorante a base de Stevia rebaudiana como palatabilizante em dietas de leitões desmamados sobre o desempenho (ganho diário de peso, consumo diário de ração e conversão alimentar e a incidência de diarreia. Foram utilizados 72 leitões, homogêneos quanto à linhagem (Large White x Landrace, desmamados aos 23 dias de idade, pesando em média 7,60 ± 0,90 kg. Foi utilizado o delineamento em blocos completos casualizados, sendo que a unidade experimental foi constituída por dois animais, submetidos a quatro tratamentos com nove repetições cada. Os animais, em grupos de dois (macho castrado e fêmea foram alojados em baias metálicas suspensas. O desempenho dos leitões foi avaliado no período I (23 a 37 dias de idade, período II (37 a 51 dias de idade, período III (51 a 65 dias de idade e período total (23 a 65 dias de idade. Os tratamentos consistiram de rações contendo níveis crescentes (0,00; 0,125; 0,250 e 0,500% de inclusão de edulcorante. Esses níveis de inclusão foram utilizados nos períodos I e II, enquanto no período III as inclusões de edulcorante foram reduzidas em 50%, ou seja, 0,00, 0,0625, 0,125 e 0,250%, respectivamente. Não ocorreram casos graves de diarreia nos primeiros 15 dias do ensaio nos tratamentos estudados. Não foram observadas diferenças significativas (P>0,05 em nenhuma das variáveis de desempenho estudadas nos períodos I, II e total. Durante o período III, houve efeito quadrático tanto no ganho diário de peso (P=0,0158 quanto na conversão alimentar (P=0,0252. A adição de palatabilizante a base de Stevia na dieta de leitões desmamados influenciou o desempenho destes dos 51 aos 65 dias de idade, sendo que o nível ótimo calculado de inclusão do edulcorante que proporcionou melhores ganho diário de peso e conversão alimentar foi de 0,265 e 0,286%, respectivamente.

  16. Diseño de galletas con alto valor nutricional para diabéticos

    OpenAIRE

    MARTÍNEZ RUESCAS, ADRIÁN

    2016-01-01

    [ES] El objetivo del presente trabajo es la formulación de un producto horneado, en concreto, de galletas con sustitución del 20% de harina de quínoa y diferentes edulcorantes como son la tagatosa y la estevia paa el mercado de pacientes diabéticos. El trabajo se realiza teniendo en cuenta la existencia de un mercado muy maduro donde se producen pocos cambios y la necesidad de ofrecer al público diabético productos que puedan consumir sin riesgo para su salud. De este modo, las...

  17. Biscuits with No Added Sugar Containing Stevia, Coffee Fibre and Fructooligosaccharides Modifies α-Glucosidase Activity and the Release of GLP-1 from HuTu-80 Cells and Serotonin from Caco-2 Cells after In Vitro Digestion.

    Science.gov (United States)

    Martinez-Saez, Nuria; Hochkogler, Christina Maria; Somoza, Veronika; Del Castillo, Maria Dolores

    2017-07-04

    This study assessed the in vitro effects of the bioaccessible food components released during the simulated human digestion of a coffee fibre-containing biscuit (CFB) on α-glucosidase activity, antioxidant capacity and satiety hormones. Digest of CFB presented a significantly (p diabetes-related digestive enzyme, and to improve the release of satiety hormones.

  18. Biscuits with No Added Sugar Containing Stevia, Coffee Fibre and Fructooligosaccharides Modifies α-Glucosidase Activity and the Release of GLP-1 from HuTu-80 Cells and Serotonin from Caco-2 Cells after In Vitro Digestion

    Science.gov (United States)

    Martinez-Saez, Nuria; Hochkogler, Christina Maria; Somoza, Veronika; del Castillo, Maria Dolores

    2017-01-01

    This study assessed the in vitro effects of the bioaccessible food components released during the simulated human digestion of a coffee fibre-containing biscuit (CFB) on α-glucosidase activity, antioxidant capacity and satiety hormones. Digest of CFB presented a significantly (p < 0.05) lower amount of sugar (68.6 mg/g) and a higher antioxidant capacity (15.1 mg chlorogenic acid eq./g) than that of a sucrose-containing biscuit (SCB). The CFB significantly reduced (p < 0.05) α-glucosidase activity (IC50 = 3.3 mg/mL) compared to the SCB (IC50 = 6.2 mg/mL). Serotonin and glucagon-like peptide-1 (GLP-1) release by differentiated Caco-2 and HuTu-80 cells, respectively, was stimulated by the CFB (355% at a concentration of 0.5 mg/mL and 278% at a concentration of 0.05 mg/mL) to the same order of magnitude as those of the SCB. To summarize, the CFB was demonstrated to reduce monosaccharide bioaccessibility, to inhibit a diabetes-related digestive enzyme, and to improve the release of satiety hormones. PMID:28677657

  19. Phytonutrients in Oat (Avena sativa L. Drink: Effect of Plant Extract on Antiradical Capacity, Nutritional Value and Sensory Characteristics

    Directory of Open Access Journals (Sweden)

    Gramza-Michałowska Anna

    2018-03-01

    Full Text Available The purpose of this study was to design the formula and antioxidant potential of gluten-free oat drinks fortified with bioactive plant components. The paper describes the use of three preparations of plant origin: yellow tea leaf extract, mulberry extract and steviol glycosides from stevia leaves. Analyses of the oat drinks basic composition – including proteins, lipids, carbohydrates, dietary fiber fractions and antioxidant properties with DPPH, ABTS, ORAC and FRAP assays were conducted. Tea extract with steviosides oat drink showed the highest total phenolic concentration and the highest antioxidant activity followed by stevia and mulberry oat drink, whereas stevia oat drink showed the lowest phenolics level and weakest antioxidant activity. Moreover, the applied plant components showed moderate ability to influence the sensory values of the fortified oat drinks. The results demonstrate that tea, stevia and mulberry preparations applied in oat drinks enhanced antioxidant potential and might be a natural source of antioxidant components.

  20. BKR 27(2) pp. 85-88 (Adekanle et al)

    African Journals Online (AJOL)

    Femi J. Olorunniji

    2015-06-30

    2010). Nutrient Composition of Cultivated. Stevia Leaves and the Influence of Polyphenols and Plant. Pigments on Sensory and Antioxidant Properties of Leaf. Extracts. Journal of Food Science and Technology, 47: 27–. 33.

  1. Steviol glycosides in food : Exposure scenarios and health effect assessment

    NARCIS (Netherlands)

    Tijhuis MJ; Wapperom D; Wolterink G; van Oosterhout CHM; Temme EHM; van Klaveren J; Verhagen H; Fransen HP; CVG; vgc

    2011-01-01

    Het gebruik van steviol glycosiden (extracten van de Stevia plant) als zoetstof in voedingsmiddelen is recent goedgekeurd door de Europese Commissie. De marktintroductie van deze producten zal waarschijnlijk niet leiden tot een gezondheidsprobleem in Nederland. Echter, extreme gebruikers van

  2. Improving the antiprotozoal effect of saponins in the rumen by combination with glycosidase inhibiting iminosugars or by modification of their chemical structure.

    Directory of Open Access Journals (Sweden)

    Eva Ramos-Morales

    Full Text Available The antiprotozoal effect of saponins is transitory, as when saponins are deglycosylated to sapogenins by rumen microorganisms they become inactive. We hypothesised that the combination of saponins with glycosidase-inhibiting iminosugars might potentially increase the effectiveness of saponins over time by preventing their deglycosylation in the rumen. Alternatively, modifying the structure of the saponins by substituting the sugar moiety with other small polar residues might maintain their activity as the sugar substitute would not be enzymatically cleaved. The aim of this in vitro study was to evaluate the acute antiprotozoal effect and the stability of this effect over a 24 h incubation period using ivy saponins, a stevia extract rich in iminosugars, ivy saponins with stevia extract, and a chemically modified ivy saponin, hederagenin bis-succinate (HBS. The effects on fermentation parameters and rumen bacterial communities were also studied. Ivy saponins with stevia and HBS had a greater antiprotozoal effect than ivy saponins, and this effect was maintained after 24 h of incubation (P<0.001. The combination of ivy and stevia extracts was more effective in shifting the fermentation pattern towards higher propionate (+39% and lower butyrate (-32% and lower ammonia concentration (-64% than the extracts incubated separately. HBS caused a decrease in butyrate (-45% and an increase in propionate (+43% molar proportions. However, the decrease in ammonia concentration (-42% observed in the presence of HBS was less than that caused by ivy saponins, either alone or with stevia. Whereas HBS and stevia impacted on bacterial population in terms of community structure, only HBS had an effect in terms of biodiversity (P<0.05. It was concluded that ivy saponins with stevia and the modified saponin HBS had a strong antiprotozoal effect, although they differed in their effects on fermentation parameters and bacteria communities. Ivy saponins combined with an

  3. LA INGENIERÍA GENÉTICA DE PLANTAS EN COLOMBIA: UN CAMINO EN CONSTRUCCIÓN

    Directory of Open Access Journals (Sweden)

    Alejandro CHAPARRO-GIRALDO

    2015-01-01

    Full Text Available Se reportan en Colombia trabajos en el desarrollo de líneas genéticamente modificadas (GM de arroz, yuca, algodón, papa, caña de azúcar, café, maíz, soya, estevia y crisantemo, por parte de cinco centros de investigación (CIAT, CENICAÑA, CENICAFE, CIB, CORPOICA y tres universidades (Universidad Nacional de Colombia, Universidad Javeriana, Universidad de Antioquia. Para llegar a la liberación comercial de variedades GM, se requiere de verdaderas políticas públicas en ciencia y tecnología, que den financiación suficiente y oportuna, así como resolver problemas con la maraña de patentes que limitan o impiden el desarrollo de innovaciones biotecnológicas, además de con el paquete regulatorio, que incrementa significativamente los costos de desarrollo.

  4. A comparison of two low-calorie sweeteners and sugar in dark chocolate on sensory attributes and emotional conceptualisations.

    Science.gov (United States)

    Lagast, Sofie; De Steur, Hans; Schouteten, Joachim J; Gellynck, Xavier

    2017-08-14

    Reducing sugar consumption is an important aspect in the prevention of and fight against obesity. A broader understanding of consumers' perceptions of low-calorie sweeteners is needed. This study examined two low-calorie sweeteners, tagatose and stevia, in comparison to sugar in dark chocolate. A total of 219 consumers participated in this study and rated overall liking and sensory attributes. Participants also listed their emotional conceptualisations upon consumption and were assessed on emotional eating behaviour and health and taste attitudes. The chocolate with tagatose was perceived as more similar to the chocolate with sugar than with stevia on overall liking, texture, bitterness, duration of aftertaste and intensity of aftertaste. Furthermore, chocolate with sugar and chocolate with tagatose both elicited positive emotional conceptualisations whereas chocolate with stevia elicited negative emotional conceptualisations. In conclusion, dark chocolate with tagatose did not significantly differ from sugar in overall liking, most sensory attributes and emotional conceptualisation.

  5. Improving the antiprotozoal effect of saponins in the rumen by combination with glycosidase inhibiting iminosugars or by modification of their chemical structure

    Science.gov (United States)

    de la Fuente, Gabriel; Nash, Robert J.; Braganca, Radek; Duval, Stephane; Bouillon, Marc E.; Lahmann, Martina; Newbold, C. Jamie

    2017-01-01

    The antiprotozoal effect of saponins is transitory, as when saponins are deglycosylated to sapogenins by rumen microorganisms they become inactive. We hypothesised that the combination of saponins with glycosidase-inhibiting iminosugars might potentially increase the effectiveness of saponins over time by preventing their deglycosylation in the rumen. Alternatively, modifying the structure of the saponins by substituting the sugar moiety with other small polar residues might maintain their activity as the sugar substitute would not be enzymatically cleaved. The aim of this in vitro study was to evaluate the acute antiprotozoal effect and the stability of this effect over a 24 h incubation period using ivy saponins, a stevia extract rich in iminosugars, ivy saponins with stevia extract, and a chemically modified ivy saponin, hederagenin bis-succinate (HBS). The effects on fermentation parameters and rumen bacterial communities were also studied. Ivy saponins with stevia and HBS had a greater antiprotozoal effect than ivy saponins, and this effect was maintained after 24 h of incubation (Pstevia extracts was more effective in shifting the fermentation pattern towards higher propionate (+39%) and lower butyrate (-32%) and lower ammonia concentration (-64%) than the extracts incubated separately. HBS caused a decrease in butyrate (-45%) and an increase in propionate (+43%) molar proportions. However, the decrease in ammonia concentration (-42%) observed in the presence of HBS was less than that caused by ivy saponins, either alone or with stevia. Whereas HBS and stevia impacted on bacterial population in terms of community structure, only HBS had an effect in terms of biodiversity (Pstevia and the modified saponin HBS had a strong antiprotozoal effect, although they differed in their effects on fermentation parameters and bacteria communities. Ivy saponins combined with an iminosugar-rich stevia extract and/or HBS should be evaluated to determine their antiprotozoal

  6. Dietary composite dry mixture in the production of flour culinary and functionality products

    Directory of Open Access Journals (Sweden)

    E. V. Belokurova

    2013-01-01

    Full Text Available Article contains information about new technologies of flour and bakery products on the basis of multicomponent structure products or the flour composite mixes executed from a flour of pumpkin and a thistle, a dry leaf of a stevia and dry lactulose, and also mixes from different types of grain, such as: corn, buckwheat, rice, millet.

  7. In vitro and in vivo evaluation of antimicrobial and antioxidant ...

    African Journals Online (AJOL)

    The acetone extract suppressed the elevation of serum ALT (p <0.05) and AST (p <0.001) activities induced by CCl4. Animals given stevia extract showed prevention against deleterious effects of CCl4 by lowering lipid peroxidation and enhancement of antioxidant activities as SOD and CAT. The protection trial is better than ...

  8. Moselhy et al., Afr J Tradit Complement Altern Med. (2016) 13(6):18-21

    African Journals Online (AJOL)

    Materials and methods: Antibacterial activity of different extracts of stevia was tested in vitro against different species of bacteria and hepato-protective efficacy was testes in ... Oxygen radicals including hydroxyl, nitrate and peroxide can induce mutation of DNA and adducts (Alia et al.,. 2006).These radicals can directly or ...

  9. African Journal of Traditional, Complementary and Alternative ...

    African Journals Online (AJOL)

    In vitro and in vivo evaluation of antimicrobial and antioxidant potential of stevia extract · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Said S. Moselhy, Magdy A. Ghoneim, Jehan A. Khan, 18-21. http://dx.doi.org/10.21010/ajtcam.v13i6.4 ...

  10. Metabolic and inflammatory responses to the common sweetener stevioside and a glycemic challenge in horses with equine metabolic syndrome.

    Science.gov (United States)

    Elzinga, S E; Rohleder, B; Schanbacher, B; McQuerry, K; Barker, V D; Adams, A A

    2017-07-01

    Extracts derived from the leaves of the stevia plant (stevioside) are commonly used as sweeteners for humans and horses. Stevioside appears to be safe for human consumption, including for individuals with insulin dysregulation. In the horse, the safety or metabolic effects of stevioside on normal animals or on those with metabolic dysfunction are unknown. Furthermore, the inflammatory response to a glycemic challenge or to stevioside in horses is not well defined. Therefore, the objective of this study was to measure the effects of stevioside and a glycemic challenge on insulin, glucose, and inflammatory responses in horses with a common metabolic dysfunction (equine metabolic syndrome or EMS) compared with non-EMS controls. To accomplish this, 15 horses were selected; 8 EMS and 7 age-matched controls. An oral sugar test was performed using Karo corn syrup (karo) or stevioside in a random crossover design. Horses were given 0.15 mL/kg body weight of karo or its equivalent grams of sugar in stevia dissolved in water. Blood samples were collected by jugular venipuncture before administration of either stevia or karo and at 60 and 240 min after administration. Serum was used for glucose and insulin determination and plasma for isolation of peripheral blood mononuclear cells (PBMCs) for inflammatory cytokine analysis via flow cytometry and reverse transcription PCR (RT-PCR). Stevia appeared to stimulate lower glycemic and insulinemic responses when compared to karo, in particular in EMS horses. EMS and control horses had inverse inflammatory responses to administration of either stevia or karo with EMS horses having a proinflammatory response (P ≤ 0.05). These data provide evidence as to why horses with EMS may be predisposed to developing laminitis, potentially as a result of an exaggerated inflammatory response to glycemic and insulinemic responses. Furthermore, the data provide new avenues for exploring mechanisms behind the syndrome, in particular when using a

  11. Sensory profile and acceptability for pitanga (Eugenia uniflora L.) nectar with different sweeteners.

    Science.gov (United States)

    Freitas, Mírian Luisa Faria; Dutra, Mariana Borges de Lima; Bolini, Helena Maria André

    2016-12-01

    The objective of this study was to evaluate the sensory properties and acceptability of pitanga nectar samples prepared with sucrose and different sweeteners (sucralose, aspartame, stevia with 40% rebaudioside A, stevia with 95% rebaudioside A, neotame, and a 2:1 cyclamate/saccharin blend). A total of 13 assessors participated in a quantitative descriptive analysis and evaluated the samples in relation to the descriptor terms. The acceptability test was carried out by 120 fruit juice consumers. The results of the quantitative descriptive analysis of pitanga nectar showed that samples prepared with sucralose, aspartame, and the 2:1 cyclamate/saccharin blend had sensory profiles similar to that of the sample prepared with sucrose. Consumers' most accepted samples were prepared with sucrose, sucralose, aspartame, and neotame. The sweeteners that have the greatest potential to replace sucrose in pitanga nectar are sucralose and aspartame. © The Author(s) 2016.

  12. Sweetener blend optimization by using mixture design methodology and the electronic tongue.

    Science.gov (United States)

    Waldrop, Megan E; Ross, Carolyn F

    2014-09-01

    Utilizing more than one sweetener has been shown to be an effective way to substitute sucrose in food products. The objective of this study was to apply the augmented simplex-centroid mixture design for the optimization of acceptable sweetener blends using coconut sugar, agave, and stevia. Sweetener blends were evaluated in aqueous solutions and gluten-free granola bars by a trained panel and consumers (n = 60). Significant differences were found between sweetener mixtures in solutions by both panelists and consumers (P mixtures. Using contour plots and desirability function analysis, an optimal sweetener combination was found for a granola bar formulation of 89.9% coconut sugar, 6.1% agave, and 4% stevia. These results indicate that a mixture design can be a reliable way to develop new sweetener blends for product development. © 2014 Institute of Food Technologists®

  13. LOW-CALORIES RAISINS OBTAINED BY COMBINED DEHYDRATION: PROCESS OPTIMIZATION AND EVALUATION OF THE ANTIOXIDANT EFFICIENCY

    Directory of Open Access Journals (Sweden)

    Mariana B. Laborde

    2015-03-01

    Full Text Available A healthy dehydrated food of high nutritional-quality and added-value was developed: low-calories raisin obtained by an ultrasonic assisted combined-dehydration with two-stage osmotic treatment (D3S complemented by drying. Pink Red Globe grape produced at Mendoza (Argentina, experienced a substitution of sugar by natural sweetener Stevia in two osmotic stages under different conditions (treatment with/without ultrasound; sweetener concentration 18, 20, 22% w/w; time 35, 75, 115 minutes, evaluating soluble solids (SS, moisture (M, total polyphenols (PF, antioxidant efficiency (AE and sugar profile. The multiple optimization of the process by response surface methodology and desirability analysis, allowed to minimize M, maximize SS (Stevia incorporation, and preserve the maximum amount of PF. After the first stage, the optimal treatment reduced the majority sugars of the grape in 32% (sucrose, glucose, and the 57% at the end of the dehydration process.

  14. Dicty_cDB: VFH157 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available of strain col-0 of Arabidopsis thaliana (thale cress). 64 3e-12 3 BX831354 |BX831354.1 Arabidopsis thaliana...of strain col-0 of Arabidopsis thaliana (thale cress). 64 4e-12 3 AY045584 |AY045584.1 Arabidopsis thaliana...of strain col-0 of Arabidopsis thaliana (thale cress). 64 4e-12 3 BG525546 |BG525546.1 51-5 Stevia field

  15. Dicty_cDB: CFB725 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available of strain col-0 of Arabidopsis thaliana (thale cress). 64 7e-13 3 BX831354 |BX831354.1 Arabidopsis thaliana...of strain col-0 of Arabidopsis thaliana (thale cress). 64 7e-13 3 AY045584 |AY045584.1 Arabidopsis thaliana...of strain col-0 of Arabidopsis thaliana (thale cress). 64 8e-13 3 BG525546 |BG525546.1 51-5 Stevia field

  16. Influencia de distintos polioles en las propiedades fisicoquímicas y sensoriales de mermelada de fresa.

    OpenAIRE

    BAIXAULI MARÍN, ELENA ASCENSIÓN

    2015-01-01

    [EN] The purpose of this dissertation was to assess the effect of different polyols (maltitol, isomalt, and erythritol) and intensive sweeteners (acesulfame potassium, aspartame, stevia, and sucralose) on physico-chemical and sensorial properties of strawberry jam. Five different formulations were developed and their compositional properties (Brix degrees, humidity, pH and water activity) and physico-chemical (optical and rheological) were evaluated during 6 months of storage a...

  17. Avaliação sensorial, microbiologia e físico-química de suco artificial de laranja adicionado de diferentes agentes adoçantes

    Directory of Open Access Journals (Sweden)

    Iago Felipe da Silva Leite

    2016-11-01

    Full Text Available Diante do aumento do consumo de adoçantes por vários fatores e a procura por uma dieta mais saudável,  para a redução dos riscos de doenças como, diabetes, obesidade, entre outras, este trabalho teve como objetivo avaliar a diferença da doçura e aceitação dos diferentes agentes adoçantes em sucos artificiais. Foram formulados sucos em pó sabor laranja e adicionado os diferentes agentes adoçantes (sacarose, demerara, stevia pura, sacarina sódica + ciclamato de sódio e sacarina sódica + ciclamato de sódio + stevia. Realizou-se analises de pH e de sólidos solúveis totais (ºBrix para as diferentes formulações, ocorrendo variações, além das análises microbiológicas para coliformes totais e Salmonella sp/25g, resultando em ausência para ambos Os resultados apontam que as amostras estão próprias para consumo segundo a legislação virgente. A análise foi aplicada no laboratório de análise sensorial da Universidade Federal de Campina Grande e avaliada por 93 provadores. Aplicou-se uma ficha sensorial levando em consideração a aceitação dos agentes adoçantes. Conclui-se que a sacarina sódica + ciclamato de sódio e sacarina sódica + ciclamato de sódio + stevia foi a formulação com maior índice de aprovação.SensoSensory, microbiological and physico-chemical evaluation of artificial orange juice added with different sweetening agents Abstract: The increase in the consumption of sweeteners by several factors. And the demand for a healthier diet with reduced risk of diseases such as, diabetes, obesity, among others, this study aimed to assess the degree of sweetness of difference and acceptance of different sweetening agents. Were formulated artificial juices powdered orange flavor and added different sweeteners (sucrose, demerara, pure stevia, saccharin + sodium cyclamate and sodium saccharin + sodium cyclamate + stevia. We conducted analysis of pH and total soluble solids (° Brix for the different

  18. DFT molecular modeling and NMR conformational analysis of a new longipinenetriolone diester

    Science.gov (United States)

    Cerda-García-Rojas, Carlos M.; Guerra-Ramírez, Diana; Román-Marín, Luisa U.; Hernández-Hernández, Juan D.; Joseph-Nathan, Pedro

    2006-05-01

    The structure and conformational behavior of the new natural compound (4 R,5 S,7 S,8 R,9 S,10 R,11 R)-longipin-2-en-7,8,9-triol-1-one 7-angelate-9-isovalerate (1) isolated from Stevia eupatoria, were studied by molecular modeling and NMR spectroscopy. A Monte Carlo search followed by DFT calculations at the B3LYP/6-31G* level provided the theoretical conformations of the sesquiterpene framework, which were in full agreement with results derived from the 1H- 1H coupling constant analysis.

  19. Time-intensity profile of pitanga nectar (Eugenia uniflora L.) with different sweeteners: Sweetness and bitterness.

    Science.gov (United States)

    Freitas, Mírian Luisa Faria; de Lima Dutra, Mariana Borges; Bolini, Helena Maria André

    2016-01-01

    Pitanga has been used by the Brazilian food industry mainly for juice production. This fruit shows good economic potential due to its high concentration of vitamins and minerals. The aim of the present work was to characterize the time-intensity profile of pitanga nectar sweetened with different sweeteners to verify differences on the perception of sweet and bitter tastes. The sweeteners used to replace sucrose were sucralose, aspartame, stevia 40% rebaudioside A, stevia 95% rebaudioside A, neotame, and 2:1 cyclamate/saccharin blend. Fifteen assessors were selected according to their discriminating capability and trained to participate in the time-intensity analysis for sweetness and bitterness. The samples prepared with sucralose and 2:1 cyclamate/saccharin blend presented a similar sweetness profile to the sample prepared with sucrose, and the samples prepared with sucralose and aspartame presented a similar bitterness profile to the sample prepared with sucrose. Thus, sucralose would be the most suitable sweetener to replace sucrose in pitanga nectar. © The Author(s) 2015.

  20. POR DESHIDRATACIÓN COMBINADA: OPTIMIZACION DEL PROCESO Y EVALUACIÓN DE LAEFICIENCIA ANTIOXIDANTE.

    Directory of Open Access Journals (Sweden)

    Mariana B. Laborde

    2015-01-01

    Full Text Available Se desarrolló un alimento deshidratado saludable de eleva da calidad nutricional y valor agregado: uva pasa de bajo contenido calórico, por deshidratación combinada asistida por ultrasonido mediante un tratamiento osmótico dual (D3S de dos etapas complementado por secado. U va rosada Red Globe producida en Mendoz a (Argentina se sometió a sustitución de sus azúcares por edulcorante natural Stevia en dos etapas osmóticas bajo diferentes condiciones ( tratamiento con/sin ultrasonido; concentración del edulcorante 18, 20, 22%p/p ; tiempo 35, 75 , 115 minutos , evaluando sólidos solubles (SS, humedad (M , polifenoles totales ( P F, eficiencia antioxidante (A E y perfil de azúcares. L a optimización múltiple del proceso por la metodología de superficie de respuesta y análisis de deseabilidad , permitió minimiza r M, maximizan do SS ( incorporación de Stevia y conservando la máxima cantidad PF. El tratamiento óptimo redujo un 32% los azúcares mayoritarios de la uva (sacarosa, glucosa tras la primera etapa, y el 57% de ellos al final del proceso.

  1. Formulating blackberry leaf mixtures for preparation of infusions with plant derived sources of sweeteners.

    Science.gov (United States)

    Komes, Draženka; Belščak-Cvitanović, Ana; Ljubičić, Ivan; Durgo, Ksenija; Cindrić, Iva Juranović; Bušić, Arijana; Vojvodić, Aleksandra

    2014-05-15

    Herbal mixtures composed of blackberry leaf and natural sweeteners (dried apples, prunes, figs, raisins, apricots, carrot and sweet potato, stevia leaves and liquorice root) were developed. Their nutritive and bioactive profile, biological activity and sensory properties were determined. Formulated mixtures exhibited lower total polyphenol content (259.09-350.00 mg GAE/L) when compared to plain blackberry leaf, but contained higher content of chlorogenic, ferulic, p-coumaric, rosmarinic acids and quercetin, as well as some macroelements (Ca, K, Mg) and microelements (Ba, Na). Stevia addition to formulated mixtures ensured higher polyphenolic content. Dried carrot exhibited the highest (0.988 g/g) and liquorice the lowest (0.087 g/g) content of total sugars but it contributed to the sweetness with 574.48 mg/L of glycyrrhizic acid derivatives. Plain blackberry leaf extract exhibited cytotoxic and antioxidative activity on human colon cancer cells. Formulated mixtures exhibited improved flavour profile and balanced sweetness in relation to plain blackberry leaf infusion. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Evaluation of the potential use of rebaudioside-A as sweetener for diet jam

    Directory of Open Access Journals (Sweden)

    Ana Cláudia Guilhen de Carvalho

    2013-09-01

    Full Text Available Sweeteners based on stevia extract contain a series of diterpene glycosides derivatives from steviol, standing out the rebaudioside-A. There is no tabletop sweeteners in the market formulated purely with rebaudioside-A yet, so its use in foods depends on the development of new products followed by physicochemical and sensory evaluations. This work presents the formulation of a diet strawberry jam dyed with cranberry juice and sweetened with rebaudioside-A purified from stevia plants of the lineage UEM-320 developed in the Centro de Estudos de Produtos Naturais da Universidade Estadual de Maringá. Evaluations of physicochemical properties, microbiological and sensory characteristics were carried out for the product in comparison with a control sweetened with equal amount of sucralose. The results showed that the physicochemical characteristics of the sample and the control are not significantly different and the supplementation with cranberry juice increased both color and total phenolic content in both samples. The sensory acceptability indicated a significant preference for the formulation sweetened with 100% of rebaudioside-A, only in the items flavor and purchase intent. We concluded that rebaudioside-A has a better sensory performance than sucralose, even this last one being 1.33 fold sweeter than rebaudioside-A.

  3. Optimization of pectinase enzyme production in Aspergillus fumigatus isolated from rotten fruits

    Directory of Open Access Journals (Sweden)

    2015-12-01

    Full Text Available Introduction: Pectinase is one of the most important industrial enzymes which was isolated from a wide variety of microorganisms such as bacteria and filamentous fungi. This enzyme has been usually used in the juice and textile industry. In this study, the isolation and optimization of pectinase-producing fungi on decaying rotten fruits were studied. Materials and methods: Isolation and screening of pectinase producing fungi have been done by plate culture on pectin medium and staining with Lugol's iodine solution. The best strain was identified by method of Pitt and Hocking as Aspergillus fumigates. The enzyme production was optimized by application of the factorial design which involves five factors, each at three levels. Five factors were carbon sources (whey, sugar, stevia and ammonium sulfate, manganese sulfate, temperature, and pH. Pectinase concentration was measured by the Miller method. Results: The results showed that the optimum condition for enzyme production was at 32 °C, PH = 6 , 3g / L manganese sulfate, 2.75g / L of ammonium sulfate, 10g / L of each carbon source (whey, stevia, and glucose. Optimum of enzyme production was observed in the presence of 1.328 mg / ml of glucose. Molecular weight of enzyme was obtained about 40 kDa by SDS-PAGE. Discussion and conclusion: The results demonstrated that this strain could grow in a wide range of carbon sources, PH and temperature. This study indicates that this strain is a good candidate for use in industrial application.

  4. Development of pitanga nectar with different sweeteners by sensory analysis: ideal pulp dilution, ideal sweetness, and sweetness equivalence

    Directory of Open Access Journals (Sweden)

    Mírian Luisa Faria Freitas

    2014-03-01

    Full Text Available The objective of this study was to develop pitanga nectar formulations in which sucrose was replaced with different sweeteners. Consumer tests were conducted with 50 fruit juice consumers, and a just-about-right scale was used to determine the ideal pulp dilution and ideal sweetness with sucrose. Furthermore, the adequate concentrations of six sweeteners were determined to obtain the equivalent sweetness of sucrose using relative to these concentrations the magnitude estimation model with 19 selected assessors. The ideal dilution test resulted in 25% pulp, and the ideal sweetness test, 10% sucrose. Sweetener concentrations to replace sucrose were 0.0160%, 0.0541%, 0.1000%, 0.0999%, 0.0017%, and 0.0360%, respectively, for sucralose, aspartame, stevia 40% rebaudioside A, stevia 95% rebaudioside A, neotame, and a 2:1 cyclamate/saccharin blend. These results can be used to prepare pitanga nectar with different sweeteners and obtain the same sweetness intensity in less caloric products than that of nectar prepared with sucrose.

  5. Características fisicoquímicas, sensoriales y reológicas de un yogur adicionado con concentrado de carambolo (Averroha carambola

    Directory of Open Access Journals (Sweden)

    Ricardo Adolfo Parra Huertas

    2014-12-01

    Full Text Available Hoy en día el consumidor busca alternativas naturales para alimentación que proporcionen beneficios para la salud a través de la innovación de productos como la adición de componentes y/o remplazo de sustituyentes; tal es el caso del carambolo y la stevia los cuales tienen efectos benéficos en la salud cuando son consumidos regularmente. Teniendo en cuenta lo anterior, el objetivo de esta investigación fue evaluar el efecto de la adición de carambolo en diferentes concentraciones a un yogur. Se elaboró yogur teniendo en cuenta tres tratamientos, al primero no se añadió carambolo, el segundo tratamiento se adicionó 20% de carambolo y el tercer tratamiento se adicionó 45% de carambolo, a cada tratamiento se adicionó 0,6% de stevia. Se incubó a 45°C; a cada uno de los tratamientos se determinó pH, sinéresis, evaluación sensorial y reológica. Se realizó un análisis estadístico aplicando ANOVA. Se encontró que la adición de carambolo en dos concentraciones en el yogur tuvo un efecto en la reducción de valores de sinéresis en comparación con yogur sin carambolo. Para el pH, los tres tratamientos tuvieron un comportamiento esperado para un yogur. En la evaluación sensorial el yogur con carambolo en menor concentración tuvo mejor aceptabilidad entre los panelistas. Reológicamente las muestras de yogur presentaron un comportamiento no newtoniano evidenciándose un mayor esfuerzo de cizalla para los muestras de yogur. Se concluye que la utilización de carambolo a bajas concentraciones y adicionándose stevia como endulzante tuvo resultados aceptables.

  6. Artificial sweeteners: safe or unsafe?

    Science.gov (United States)

    Qurrat-ul-Ain; Khan, Sohaib Ahmed

    2015-02-01

    Artificial sweeteners or intense sweeteners are sugar substitutes that are used as an alternative to table sugar. They are many times sweeter than natural sugar and as they contain no calories, they may be used to control weight and obesity. Extensive scientific research has demonstrated the safety of the six low-calorie sweeteners currently approved for use in foods in the U.S. and Europe (stevia, acesulfame-K, aspartame, neotame, saccharin and sucralose), if taken in acceptable quantities daily. There is some ongoing debate over whether artificial sweetener usage poses a health threat .This review article aims to cover thehealth benefits, and risks, of consuming artificial sweeteners, and discusses natural sweeteners which can be used as alternatives.

  7. Influence of hydrogenated oil as cocoa butter replacers in the development of sugar-free compound chocolates: Use of inulin as stabilizing agent.

    Science.gov (United States)

    Rodriguez Furlán, Laura T; Baracco, Yanina; Lecot, Javier; Zaritzky, Noemi; Campderrós, Mercedes E

    2017-02-15

    The effect of the addition of inulin as a surfactant or stability agent on white compound chocolate sweetened with sucralose and Stevia was studied. Samples were stored at 7, 15 and 30°C during 100days and the influence of inulin on rheological properties, sensorial attributes, shelf-life, physical properties such as melting, crystallization and blooming were analyzed. The shelf-life of the compound chocolate with the incorporation of inulin was higher than the control sample without replacement. Compound chocolate with inulin at 10%w/w showed a dense matrix structure, reducing the size and number of fat crystals formed during storage; furthermore they presented higher values of brightness and WI. This chocolate also showed less fracturability and improved thermal properties. DSC studies revealed increased values of onset and peak temperatures and enthalpy of melting of the polymorphic form V, at higher storage temperatures, achieving greater stability against degradation processes. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. Natural sweeteners in a human diet.

    Science.gov (United States)

    Grembecka, Małgorzata

    2015-01-01

    Sweeteners, both natural and artificial, play an important role in a human diet as well as are of great importance to the food industry and dieticians. Many people associate sweet taste with sucrose, which is commonly known as table sugar. However, there are many sweet substances that food manufacturers add to food products because none of them is ideal for all applications. Besides sucrose there are also other sugars such as glucose and fructose that originate both from natural sources such as fruits and honey or from added sugars. Among sweeteners there are also compounds which have a sweet taste and contain no calories or those which sweetness is so intense so can be used at very low concentrations, thus, their impact on the total caloric value of the product is negligible. They can be classified due to their origin (natural or synthetic agents), the technological function (sweeteners and fillers), texture (powders and syrups), and nutritional value (caloric and non-caloric). Natural sweetening substances include carbohydrates, sugar alcohols, thaumatin and stevia. Besides providing well tasting foods, they might have an impact on products' texture, color, preservation and caloric value. Sugar alcohols, which belong to carbohydrates, are both natural sugar substitutes and food additives. They are becoming more and more popular among consumers mainly due to their lower caloric values and glycemic indexes as well as anticariogenic effects. Sugar alcohols are often combined with other sweeteners to enhance food products' sweetness. Stevia, which is 200 times sweeter than sucrose, is a non caloric substance whereas thaumatin, a sweet protein, provides 4 kcal/g but characterizes with sweetness about 2000 times higher than sucrose (on a weight basis).

  9. Effect of moderate intake of sweeteners on metabolic health in the rat.

    Science.gov (United States)

    Figlewicz, D P; Ioannou, G; Bennett Jay, J; Kittleson, S; Savard, C; Roth, C L

    2009-12-07

    The rise in prevalence of obesity, diabetes, metabolic syndrome, and fatty liver disease has been linked to increased consumption of fructose-containing foods or beverages. Our aim was to compare the effects of moderate consumption of fructose-containing and non-caloric sweetened beverages on feeding behavior, metabolic and serum lipid profiles, and hepatic histology and serum liver enzymes, in rats. Behavioral tests determined preferred (12.5-15%) concentrations of solutions of agave, fructose, high fructose corn syrup (HFCS), a combination of HFCS and Hoodia (a putative appetite suppressant), or the non-caloric sweetener Stevia (n=5/gp). HFCS intake was highest, in preference and self-administration tests. Groups (n=10/gp) were then assigned to one of the sweetened beverages or water as the sole source of liquid at night (3 nights/wk, 10wks). Although within the normal range, serum cholesterol was higher in the fructose and HFCS groups, and serum triglycerides were higher in the Agave, HFCS, and HFCS/Hoodia groups (vs. water-controls, pStevia, agave, fructose, HFCS, and water-consuming groups, however levels of IL-6 were significantly lower in association with the ingestion of Hoodia. There were no differences in terminal body weights, or glucose tolerance assessed by 120-min IVGTTs performed at the end of the 10-week regimen. We conclude that even moderate consumption of fructose-containing liquids may lead to the onset of unfavorable changes in the plasma lipid profile and one marker of liver health, independent of significant effects of sweetener consumption on body weight.

  10. Influence of Package Visual Cues of Sweeteners on the Sensory-Emotional Profiles of Their Products.

    Science.gov (United States)

    Wardy, Wisdom; Chonpracha, Pitchayapat; Chokumnoyporn, Napapan; Sriwattana, Sujinda; Prinyawiwatkul, Witoon; Jirangrat, Wannita

    2017-02-01

    Substantial evidence suggests influence of color, physical state, and other extrinsic features on consumer perception and acceptability of food products. In this study, 560 subjects evaluated liking and emotional responses associated with 5 sweeteners (sucralose, stevia, saccharin, aspartame, and sucrose) under 2 eliciting conditions: control (brand name only) and informed (brand name/packet image), to assess impact of the packet color. For a given condition, 5 identical tea samples each labeled with a sweetener type were rated for sweetness and overall liking (9-point) and emotions (5-point). Nonsignificant interactions between eliciting condition and sweetener type were found for liking attributes and emotions (except peaceful), indicating their independent effects. However, overall differences existed among sweetener types and eliciting conditions based on both hedonic and emotional responses (MANOVA, P < 0.05), suggesting modulating effects of packet color on sweetener type in the sensory-emotion space. The sensory-emotion profile for sucrose was separate from that of nonnutritive sweeteners, with statistically significant Mahalanobis distances among sample centroids. Increases in positive emotion intensities contrasted with a decrease in negative emotion intensities were observed for some sweeteners moving from the control to informed condition. Sweetness liking was strongly correlated with the emotion satisfied (sucralose, saccharin) only in the control condition, whereas it was strongly correlated with the emotions pleased and satisfied (stevia), disgusted (aspartame), and satisfied (sucrose) only in the informed condition. Overall, results suggested that sensory liking and emotions during the consumption experience are related not entirely to the type of sweetener, but also the color of the packet. © 2017 Institute of Food Technologists®.

  11. Rehydration with soft drink-like beverages exacerbates dehydration and worsens dehydration-associated renal injury.

    Science.gov (United States)

    García-Arroyo, Fernando E; Cristóbal, Magdalena; Arellano-Buendía, Abraham S; Osorio, Horacio; Tapia, Edilia; Soto, Virgilia; Madero, Magdalena; Lanaspa, Miguel A; Roncal-Jiménez, Carlos; Bankir, Lise; Johnson, Richard J; Sánchez-Lozada, Laura-Gabriela

    2016-07-01

    Recurrent dehydration, such as commonly occurs with manual labor in tropical environments, has been recently shown to result in chronic kidney injury, likely through the effects of hyperosmolarity to activate both vasopressin and aldose reductase-fructokinase pathways. The observation that the latter pathway can be directly engaged by simple sugars (glucose and fructose) leads to the hypothesis that soft drinks (which contain these sugars) might worsen rather than benefit dehydration associated kidney disease. Recurrent dehydration was induced in rats by exposure to heat (36°C) for 1 h/24 h followed by access for 2 h to plain water (W), a 11% fructose-glucose solution (FG, same composition as typical soft drinks), or water sweetened with noncaloric stevia (ST). After 4 wk plasma and urine samples were collected, and kidneys were examined for oxidative stress, inflammation, and injury. Recurrent heat-induced dehydration with ad libitum water repletion resulted in plasma and urinary hyperosmolarity with stimulation of the vasopressin (copeptin) levels and resulted in mild tubular injury and renal oxidative stress. Rehydration with 11% FG solution, despite larger total fluid intake, resulted in greater dehydration (higher osmolarity and copeptin levels) and worse renal injury, with activation of aldose reductase and fructokinase, whereas rehydration with stevia water had opposite effects. In animals that are dehydrated, rehydration acutely with soft drinks worsens dehydration and exacerbates dehydration associated renal damage. These studies emphasize the danger of drinking soft drink-like beverages as an attempt to rehydrate following dehydration. Copyright © 2016 the American Physiological Society.

  12. The Effect of Rebadioside A on Attenuation of Oxidative Stress in Kidney of Mice under Acetaminophen Toxicity

    Directory of Open Access Journals (Sweden)

    Seyed Ali Hashemi

    2014-11-01

    Full Text Available Background: Acetaminophen (APAP overdose causes renal and hepatic injury. It is also believed that oxidative stress has a pivotal role in APAP-induced renal injury. Therefore, protective effects of different antioxidants have been examined in APAP-induced renal and hepatic toxicity models. Stevia rebadiana is a plant with a high degree of natural antioxidant activity in its leaf extract. The aim of this study was to investigate the possible protective effects of rebadioside A; one of the main components of stevia extract, on APAP-induced oxidative stress in kidney of mice. Methods: Oxidative stress was induced in kidney of BALB/c mice by the intraperitoneal (i.p. administration of a single dose of 300 mg/kg APAP. Some of these mice also received rebadioside A (700 mg/kg (i.p. 30 minutes after APAP injection. Two and six hours after APAP injection, all mice were sacrificed and malondialdehyde (MDA, glutathione (GSH, free APAP, and glutathione conjugated of APAP (APAP-GSH were determined in the kidney tissue. Results: GSH depletion and MDA levels significantly (P<0.05 increased in mice treated with either APAP or APAP plus Rebadioside A, respectively in 2 and 6 hours intervals after APAP administration. Significantly (P<0.05 higher levels of free APAP and APAP-GSH levels detected in kidney of mice administrated with APAP plus rebadioside A compared to APAP treated ones. Conclusion: Rebadioside A may be a potential compound in alleviation of APAP-induced oxidative stress in kidney of mice after APAP overdoses.

  13. Non-nutritive sweeteners and their role in the gastrointestinal tract.

    Science.gov (United States)

    Brown, Rebecca J; Rother, Kristina I

    2012-08-01

    Non-nutritive sweeteners can bind to sweet-taste receptors present not only in the oral cavity, but also on enteroendocrine and pancreatic islet cells. Thus, these sweeteners may have biological activity by eliciting or inhibiting hormone secretion. Because consumption of non-nutritive sweeteners is common in the United States, understanding the physiological effects of these substances is of interest and importance. A PubMed (1960-2012) search was performed to identify articles examining the effects of non-nutritive sweeteners on gastrointestinal physiology and hormone secretion. The majority of in vitro studies showed that non-nutritive sweeteners can elicit secretion of gut hormones such as glucagon-like peptide 1 and glucose-dependent insulinotropic peptide in enteroendocrine or islet cells. In rodents, non-nutritive sweeteners increased the rate of intestinal glucose absorption, but did not alter gut hormone secretion in the absence of glucose. Most studies in humans have not detected effects of non-nutritive sweeteners on gut hormones or glucose absorption. Of eight human studies, one showed increased glucose-stimulated glucagon-like peptide 1 secretion after diet soda consumption, and one showed decreased glucagon secretion after stevia ingestion. In humans, few studies have examined the hormonal effects of non-nutritive sweeteners, and inconsistent results have been reported, with the majority not recapitulating in vitro data. Further research is needed to determine whether non-nutritive sweeteners have physiologically significant biological activity in humans.

  14. Influence of temperature and fat content on ideal sucrose concentration, sweetening power, and sweetness equivalence of different sweeteners in chocolate milk beverage.

    Science.gov (United States)

    Paixão, J A; Rodrigues, J B; Esmerino, E A; Cruz, A G; Bolini, H M A

    2014-12-01

    The introduction of new products catering to specific dietary needs and the corresponding changes in the consumer profile reflect a growing demand for diet and “light” products. However, little information is available regarding the sensory effects of different sweeteners in products consumed at different temperatures and with varying fat contents. In this regard, this study aimed to determine the influence of temperature and fat content on the ideal sucrose concentration and the sweetness equivalence and sweetening power of different sweeteners: Neotame (NutraSweet Corp., Chicago, IL), aspartame, neosucralose, sucralose, and stevia (95% rebaudioside A), with sucrose as reference, in a chocolate milk beverage using a just-about-right (JAR) scale and magnitude estimation. Increasing temperature of consumption had an inverse effect on the ideal sucrose concentration in whole milk beverages, whereas no difference was noted in beverages made skim milk. In addition, a decrease in sweetening power was observed for all of the sweeteners analyzed considering the same conditions. The findings suggest that different optimal conditions exist for consumption of chocolate milk beverage related to sweetness perception, which depends on the fat level of milk used in the formulation. This information can be used by researchers and dairy processors when developing chocolate milk beverage formulations.

  15. Development of chocolate dairy dessert with addition of prebiotics and replacement of sucrose with different high-intensity sweeteners.

    Science.gov (United States)

    Morais, E C; Morais, A R; Cruz, A G; Bolini, H M A

    2014-05-01

    The aims of this study were (1) to optimize the formulation of a prebiotic chocolate dairy dessert and assess the extent to which sensory properties were affected by adding different concentrations of prebiotics (inulin and fructooligosaccharides) combined with different levels of xanthan and guar gums, and (2) to analyze the ideal and relative sweetness of prebiotic chocolate milk dessert sweetened with different artificial and natural sweeteners. Acceptability was evaluated by 100 consumers using a 9-cm hedonic scale, and the level of sample creaminess was evaluated using a 9-point just-about-right (JAR) scale. Data were subjected to a multivariate regression analysis and fitted to a model provided by response surface methodology. The optimal concentrations were 7.5% (wt/wt) prebiotic and 0.20% (wt/wt) gum (guar and xanthan, in a 2:1 ratio). The ideal sweetness analysis revealed that the ideal concentration of sucrose was 8.13%. The relative sweetness analysis showed that Neotame (NutraSweet Corp., Chicago, IL) had the highest sweetening power compared with the prebiotic chocolate dairy dessert containing 8% sucrose, followed by sucralose, aspartame, and stevia. The study of sweetness in this product is important because consumers desire healthier functional products with no added sugar. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  16. [Identification and biotransformation properties of a bacterium that converts stevioside into rubusoside].

    Science.gov (United States)

    Jiang, Zhongyu; Chen, Yuru; Liu, Hu

    2011-01-01

    The purpose of our study was to separate and identify a bacillus that could convert stevioside specifically. Then we identified the conversion product and studied the conversion capability of the bacillus. We also studied the enzyme with conversion capability and the conversion characteristic of the enzyme. The bacillus was identified on the basis of morphology features and 16S rDNA sequence analysis. Phylogenetic tree was constructed to determine its taxonomic status. The product was detected and identified by high performance liquid chromatography and liquid chromatography-mass spectrometry methods. We use bacteria media directly to studied the conversion capability of the bacillus, and use resting cells, extracellular fluid and intracellular fluid to convert stevioside to determine the enzyme and made further study to learn its conversion characteristic. The 16S rDNA sequence of the strain had 99% similarity with Chryseobacterium sp., which was ultimately identified as Chryseobacterium sp., JH. The product of biotransformation was rubusoside and the enzyme that converts stevioside into rubusoside was intracellular enzyme. The conversion rate could reach 100%, obtained 5.7 g/L rubusodide solution after 48 h by bacteria media when the concentration of stevia glycosides was 10 g/L, including 7.2 g/L stevioside. The isolated strain JH was identified as Chryseobacterium sp. It was a novel strain with high, specific ability to convert stevioside into rubusoside which had potential applications.

  17. Influence of high intensity sweeteners and sugar alcohols on a beverage microemulsion.

    Science.gov (United States)

    Marcus, Julien; Wolfrum, Stefan; Touraud, Didier; Kunz, Werner

    2015-12-15

    The present paper shows the effects of added sugars and sweeteners on the clearing temperature of a highly water dilutable fatty acid salt microemulsion used as a model of a beverage concentrate. There is a twofold interest in this work. The first one is practical and relates to the fact that many fatty acid salt surfactants can be used in food without major regulatory restrictions. As is shown here, they allow making highly stable microemulsions even at neutral and acidic pH. The second one is more of scientific interest. The model system can be used to study the effect of sugars and sweeteners on the formulation stability depending on their charges, amphiphilic properties, and localization in the microemulsion interfacial film. An important practical result is the discovery of the possibility to formulate highly dilutable microemulsions at neutral or slightly acid pH with a good taste in presence of sucralose. Further, a significant decrease of the pKA of the fatty acid is observed in presence of stevia, thus allowing transparent, fairly stable systems at neutral pH. Copyright © 2015 Elsevier Inc. All rights reserved.

  18. Biological fate of low-calorie sweeteners.

    Science.gov (United States)

    Magnuson, Bernadene A; Carakostas, Michael C; Moore, Nadia H; Poulos, Sylvia P; Renwick, Andrew G

    2016-11-01

    With continued efforts to find solutions to rising rates of obesity and diabetes, there is increased interest in the potential health benefits of the use of low- and no-calorie sweeteners (LNCSs). Concerns about safety often deter the use of LNCSs as a tool in helping control caloric intake, even though the safety of LNCS use has been affirmed by regulatory agencies worldwide. In many cases, an understanding of the biological fate of the different LNSCs can help health professionals to address safety concerns. The objectives of this review are to compare the similarities and differences in the chemistry, regulatory status, and biological fate (including absorption, distribution, metabolism, and excretion) of the commonly used LNCSs: acesulfame potassium, aspartame, saccharin, stevia leaf extract (steviol glycoside), and sucralose. Understanding the biological fate of the different LNCSs is helpful in evaluating whether reports of biological effects in animal studies or in humans are indicative of possible safety concerns. Illustrations of the usefulness of this information to address questions about LNCSs include discussion of systemic exposure to LNCSs, the use of sweetener combinations, and the potential for effects of LNCSs on the gut microflora. © The Author(s) 2016. Published by Oxford University Press on behalf of the International Life Sciences Institute. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  19. Non-nutritive sweeteners: evidence for benefit vs. risk.

    Science.gov (United States)

    Gardner, Christopher

    2014-02-01

    Intake of added sugars in the American diet is high and has been linked to weight gain and adverse effects on glycemic control and diabetes. Several national health organizations recommend decreasing added sugars intake. Among the many strategies to consider to achieve this reduction is substitution with non-nutritive sweeteners (NNS - artificial sweeteners and stevia). The purpose of this review is to critically examine existing evidence for this strategy. Short-term intervention studies suggest that NNS, when substituted for added sugars, may be useful in supporting energy intake reduction, and promoting glycemic control and weight management. However, the magnitude of effect in these studies has ranged from modest to null. Compensatory eating behaviors likely diminish, and in some cases negate, potential effects. Findings from longer-term observational studies that examine associations between NNS use and obesity or type 2 diabetes are potentially confounded by reverse causality. Existing data are insufficient to clearly support or refute the effectiveness of substitution with NNS as a means of reducing added sugar intake. It is important to not lose sight of the impact of incorporating NNS-containing beverages and foods on overall diet quality when assessing potential health benefits vs. risks.

  20. The Evaluation of saccharose replacing by adding stevioside-maltodextrin mixture on the physicochemical and sensory properties of Naanberenji (an Iranian confectionary).

    Science.gov (United States)

    Ghandehari Yazdi, Amirpouya; Hojjatoleslamy, Mohammad; Keramat, Javad; Jahadi, Mahshid; Amani, Elahe

    2017-07-01

    Stevia is a natural, non-nutritive sweetener can replace sugar in your diet to control diabetes and aid in weight loss. Naanberenji, an Iranian traditional cookie, is a well-reputed confectionary containing high sucrose and calorie value, for this reason its consumption in healthy diet has been restricted. In this study, the effect of sucrose replacement by stevioside-maltodextrin mixture on physicochemical properties of Naanberenji was evaluated in four replacing levels including 25%, 50%, 75%, and 100%. Results of texture evaluation revealed that hardness increases due to adding stevioside-maltodextrin (p < .05). Color analysis also showed that browning index by adding more mixture reduced. Moreover, the results of sensory analysis showed that treatment containing 25% stevioside-maltodextrin was the most similar sample to control and 23.06% reduction in sugar consumption achieved in this level of replacement. Therefore, its physicochemical properties (peroxide, acidity, chemical compounds, and hardness) were measured. According to the results, calorie amount decreased by 7.27 %. While there was no significant difference in the acidity of the aforesaid sample, peroxide results exhibited significant differences. Analysis of Farinograph plot revealed more water binding in comparison to control sample.

  1. Artificial sweeteners as a sugar substitute: Are they really safe?

    Science.gov (United States)

    Sharma, Arun; Amarnath, S; Thulasimani, M; Ramaswamy, S

    2016-01-01

    Nonnutritive sweeteners (NNS) have become an important part of everyday life and are increasingly used nowadays in a variety of dietary and medicinal products. They provide fewer calories and far more intense sweetness than sugar-containing products and are used by a plethora of population subsets for varying objectives. Six of these agents (aspartame, saccharine, sucralose, neotame, acesulfame-K, and stevia) have previously received a generally recognized as safe status from the United States Food and Drug Administration, and two more (Swingle fruit extract and advantame) have been added in the recent years to this ever growing list. They are claimed to promote weight loss and deemed safe for consumption by diabetics; however, there is inconclusive evidence to support most of their uses and some recent studies even hint that these earlier established benefits regarding NNS use might not be true. There is a lack of properly designed randomized controlled studies to assess their efficacy in different populations, whereas observational studies often remain confounded due to reverse causality and often yield opposite findings. Pregnant and lactating women, children, diabetics, migraine, and epilepsy patients represent the susceptible population to the adverse effects of NNS-containing products and should use these products with utmost caution. The overall use of NNS remains controversial, and consumers should be amply informed about the potential risks of using them, based on current evidence-based dietary guidelines.

  2. The influence of sweeteners in probiotic Petit Suisse cheese in concentrations equivalent to that of sucrose.

    Science.gov (United States)

    Esmerino, E A; Cruz, A G; Pereira, E P R; Rodrigues, J B; Faria, J A F; Bolini, H M A

    2013-09-01

    As in the case of probiotic functional foods in recent years, demand has increased notably for light or diet foods with added sweeteners. However, little is known about the effect of different sweeteners on the microorganisms present. Thus, the objective of the current study was to establish the ideal sucrose concentration and equivalent concentrations of different sweeteners and to determine, by microbiological analyses, the influence of these compounds on the viability of the starter and probiotic cultures used in the production of strawberry-flavored Petit Suisse cheese during its shelf life. The ideal sucrose concentration was determined using the just-about-right (JAR) scale, and the equivalent concentrations of the sweeteners were subsequently determined by the magnitude estimation method. Microbiological analyses were also carried out to check the viability of the cultures during the product's shelf life. The results showed that the compounds Neotame (NutraSweet, Chicago, IL) and stevia presented, respectively, the greatest and least sweetening power of the sweeteners tested. None of the sweeteners used in this study exerted a negative effect on the viability of the starter or probiotic cultures, and thus we were able to obtain a probiotic, functional food with reduced calorie content. Copyright © 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  3. Historical parallels of biological space experiments from Soyuz, Salyut and Mir to Shenzhou flights

    Science.gov (United States)

    Nechitailo, Galina S.; Kondyurin, Alexey

    2016-07-01

    Human exploitation of space is a great achievement of our civilization. After the first space flights a development of artificial biological environment in space systems is a second big step. First successful biological experiments on a board of space station were performed on Salyut and Mir stations in 70-90th of last century such as - first long time cultivation of plants in space (wheat, linen, lettuce, crepis); - first flowers in space (Arabidopsis); - first harvesting of seeds in space (Arabidopsis); - first harvesting of roots (radish); - first full life cycle from seeds to seeds in space (wheat), Guinness recorded; - first tissue culture experiments (Panax ginseng L, Crocus sativus L, Stevia rebaundiana B; - first tree growing in space for 2 years (Limonia acidissima), Guinness recorded. As a new wave, the modern experiments on a board of Shenzhou Chinese space ships are performed with plants and tissue culture. The space flight experiments are now focused on applications of the space biology results to Earth technologies. In particular, the tomato seeds exposed 6 years in space are used in pharmacy industry in more then 10 pharmaceutical products. Tissue culture experiments are performed on the board of Shenzhou spaceship for creation of new bioproducts including Space Panax ginseng, Space Spirulina, Space Stetatin, Space Tomato and others products with unique properties. Space investments come back.

  4. Sensory and physicochemical evaluation of acerola nectar sweetened with sucrose and different sweeteners

    Directory of Open Access Journals (Sweden)

    Mariana Borges de Lima Dutra

    2013-12-01

    Full Text Available This paper aims to evaluate the physicochemical characteristics, sensory acceptance, and purchase intent of acerola nectar sweetened with sucrose and other sweeteners (neotame, sucralose and stevia extracts with 40%, 60%, 80%, and 95% rebaudioside A. The analyses were carried out for pH, soluble solids, total titratable acidity, ascorbic acid, and colorimetry (L*a*b. The acceptance test was performed by 120 consumers who evaluated the appearance, aroma, flavor, texture, and overall impression of the samples using a 9-cm unstructured hedonic scale. Furthermore, the consumers were asked to rate overall purchase intent along the scale anchored with (1 "would definitely not purchase" to (5 "would definitely purchase." The results were evaluated using analysis of variance/Tukey test and the internal preference mapping technique. The acerola nectar samples did not differ significantly (p>0.05 between themselves in terms of vitamin C content and total titratable acidity. As for appearance and aroma, there was no significant difference (p>0.05 between the samples, and as for flavor and overall impression, the most accepted samples were those with sucrose and sucralose. The internal preference mapping indicated that the most accepted samples were those with sucrose, sucralose, and neotame were. The highest frequency of positive purchase intent scores was observed for sucrose and sucralose.

  5. Effect of sweetener combination and storage temperature on physicochemical properties of sucrose free white chocolate.

    Science.gov (United States)

    Rodriguez Furlán, Laura T; Baracco, Yanina; Lecot, Javier; Zaritzky, Noemi; Campderrós, Mercedes E

    2017-08-15

    The influence of a combination of sweeteners (Stevia (St) and sucralose (Su)) and storage temperature on thermal properties, microstructure, water content, texture and Bloom of sucrose free white chocolate was investigated. A strong relationship between the microstructure and the highest percentage of Bloom was observed. The samples with 100%Su and 50%S+50%Su presented microstructures with channels through which solids and fat could more easily spread to the surface, increasing the fat and sugar Bloom formation. However, 50%St+50%Su and 75%St+25%Su samples showed a minimum Bloom formation, probably due to its dense microstructure with no void spaces. The differential scanning calorimetry studies demonstrated that the samples containing 100%St and 75%St+25%Su showed the smallest decrease of melting enthalpy with increasing temperature. Besides, non-isothermal crystallization kinetics was studied by applying Avrami model. The sample 75%St+25%Su presented the highest values of activation energy showing the greatest stability in the temperature range studied (7°C-30°C). Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. EDULCORANTES NATURALES UTILIZADOS EN LA ELABORACIÓN DE CHOCOLATES

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    ESTEBAN PALACIO VÁSQUEZ

    2017-12-01

    Full Text Available The demand of confectionery products and their massive consumption, leads to the agri-food industry include in its transformation processes inputs that improve the nutritional quality of the final products. Considering that chocolate has a high percentage of sugar (sucrose content of 30-60%, it is necessary to use alternative sweeteners for its elaboration, that promote a healthy diet. The objective of the present review is to characterize some natural sweeteners such as erythritol, stevia (stevioside and rebaudioside-A, thaumatin and bulking agents such as inulin and polydextrose with structural and rheological functions; highlighting aspects such as its origin, caloric intake, sweetening power, ADI (Admissible Daily Intake and its influence on chocolate quality parameters. Although they may affect their sensory properties, these sweeteners have wide advantages because of their low caloric content and high potency in sweetness compared to sucrose. This shows the need to perform studies to determine the sweetener and the bulking agent that best substitute sucrose in the manufacture of chocolates. Finally, it is evident that the use of natural sweeteners, generates health benefits and impact on the sensorial and rheological quality of chocolate.

  7. Noncaloric Sweeteners in Children: A Controversial Theme

    Directory of Open Access Journals (Sweden)

    Samuel Durán Agüero

    2018-01-01

    Full Text Available Noncaloric sweeteners (NCS are food additives used to provide sweetness without adding calories. Their consumption has become more widespread around the world in all age groups, including children. The aim of this study is to show the state of the art about the intake of noncaloric sweeteners in children, as well as their benefits and consumption risk. Scientific searchers were used (PUBMED, Scopus, and Scielo to analyze articles that included keywords (noncaloric sweeteners/saccharin/cyclamate/acesulfame potassium/aspartame/sucralose/stevia/children in English, Spanish, and Portuguese. Authors conclude that it is imperative that health professionals judiciously and individually evaluate the overall benefits and risks of NCS use in consumers before recommending their use. Different subgroups of the population incorporate products containing NCS in their diet with different objectives, which should be considered when recommending a diet plan for the consumer. In childhood, in earlier age groups, this type of additives should be used as a dietary alternative when other forms of prevention in obesity are not sufficient.

  8. The safety and regulation of natural products used as foods and food ingredients.

    Science.gov (United States)

    Abdel-Rahman, Ali; Anyangwe, Njwen; Carlacci, Louis; Casper, Steve; Danam, Rebecca P; Enongene, Evaristus; Erives, Gladys; Fabricant, Daniel; Gudi, Ramadevi; Hilmas, Corey J; Hines, Fred; Howard, Paul; Levy, Dan; Lin, Ying; Moore, Robert J; Pfeiler, Erika; Thurmond, T Scott; Turujman, Saleh; Walker, Nigel J

    2011-10-01

    The use of botanicals and dietary supplements derived from natural substances as an adjunct to an improved quality of life or for their purported medical benefits has become increasingly common in the United States. This review addresses the safety assessment and regulation of food products containing these substances by the U.S. Food and Drug Administration (FDA). The issue of safety is particularly critical given how little information is available on the toxicity of some of these products. The first section uses case studies for stevia and green tea extracts as examples of how FDA evaluates the safety of botanical and herbal products submitted for consideration as Generally Recognized as Safe under the Federal Food, Drug, and Cosmetics Act. The 1994 Dietary Supplement Health Education Act (DSHEA) created a regulatory framework for dietary supplements. The article also discusses the regulation of this class of dietary supplements under DSHEA and addresses the FDA experience in analyzing the safety of natural ingredients described in pre-market safety submissions. Lastly, we discuss an ongoing interagency collaboration to conduct safety testing of nominated dietary supplements.

  9. PLANTAS MEDICINALES USADAS POR LOS COGUI EN EL RÍO PALOMINO, SIERRA NEVADA DE SANTA MARTA (COLOMBIA

    Directory of Open Access Journals (Sweden)

    EDUINO CARBONÓ-DELAHOZ

    2013-12-01

    Full Text Available Con el propósito de registrar conocimientos tradicionales sobre plantas medicinales entre los Cogui o Kággaba en la cuenca del río Palomino, Sierra Nevada de Santa Marta (Colombia, se evaluó el uso de la flora con la aplicación de una técnica etnobotánica cuantitativa. Mediante entrevistas a mamos o sacerdotes-jefes, durante recorridos en áreas silvestres, cultivadas y huertos caseros en diferentes zonas climáticas de la cuenca, se obtuvo información de 189 especies utilizadas, incluidas en 162 géneros y 77 familias, así como el modo de uso y la forma de preparación para el tratamiento de enfermedades comunes. Las familias Asteraceae y Leguminosae registraron el mayor número de especies útiles. La evaluación con el índice de importancia relativa (IR permitió establecer que Ladenbergia moritziana (Rubiaceae, Stevia lucida (Asteraceae, Aniba sp. (Lauraceae, Erechtites hieracifolia (Asteraceae y Carex sp. (Cyperaceae son las especies con mayores valores de importancia. El 89 % de las plantas utilizadas es obtenido de espacios silvestres, lo que sugiere un conocimiento amplio de la diversidad vegetal local y el ejercicio de una práctica etnomédica.

  10. de uma indústria do setor alimentício

    Directory of Open Access Journals (Sweden)

    César Renato Ferreira da Costa

    2007-01-01

    Full Text Available Pursuing opportunities is one of the few explored fields on entrepreneurship research studies. The entrepreneurial process has started from one or several individuals about one idea to be explored. Factors such as access to information and cognitive system influence such mechanism, guiding behaviors towards the creation of values. The individual’s role is extremely important for success achievement. Thus, this paper introduces an approach of pursuing opportunities according to one entrepreneur’s view. The choice of the case study was based on the innovative character of the opportunity pursued by the entrepreneur, subject of this study. This paper is framed as a case study with oral history report, through which the entrepreneurial behavior and the sense of opportunity are inferred in accordance with the production and industrialization of diet-light food. The initial process of research and development was carried out by a kind of hatching in a university in the south of Brazil, when the Stevia Rebaudian plant, found in the Brazilian and Paraguayan flora, was studied.

  11. Determination of essential elements in dietetic sample by neutron activation analysis; Determinacao de elementos essenciais em alimentos dieteticos pela tecnica de analise por ativacao com neutrons

    Energy Technology Data Exchange (ETDEWEB)

    Siquelli, Murilo V.; Maihara, Vera A. Maihara [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Lab. de Analise por Ativacao Neutronica]. E-mail: murilo_siquelli@hotmail.com; vmaihara@ipen.br

    2005-07-01

    In the last years there has been an increase of the dietetic product consumption by people who suffer from diabetes, heart disease and by people concerned about having a healthy life as well. Despite the increase of dietetic product presents in the diet of the Brazilian population, the use of these products is still controversial. The analysis of the nutritional composition of these products is becoming important because a great number of people is changing their traditional food by dietetic products. In the literature, there is no information about the inorganic composition, mainly related to the essential elements, in the dietetic products: diet and light . In this study are presented preliminary results of the concentrations of Br, Ca, Cr, Fe, Na and Zn determined by Instrumental Neutron Activation Analysis in aspartame, saccharin and cyclamate sodium , and stevia based sweetener samples. Gelatin samples, diet and light, were also analyzed. Methodology validation was done analyzing NIST reference materials Tea Leaves (INCT-TL-1) and Mixed Polish Herbs (INCT-MPH-2). (author)

  12. O uso de adoçantes na gravidez: uma análise dos produtos disponíveis no Brasil The use of sweeteners in pregnancy: an analysis of products available in Brazil

    Directory of Open Access Journals (Sweden)

    Maria Regina Torloni

    2007-05-01

    has been extensively studied in animals and it is considered safe for use during pregnancy (risk B, except by women homozygous for phenylketonuria (risk C. Sucralose and acessulfame-K are not toxic, carcinogenic or mutagenic in animals, but there are no controlled studies in humans. However, since these two sweeteners are not metabolized, it is unlikely that their use during pregnancy could be harmful (risk B. Stevia, a substance extracted from a native Brazilian plant, is innocuous in animal pregnancies, but there are no controlled studies in humans (risk B. Body agents found in the composition of artificial sweeteners (mannitol, sorbitol, xylitol, erithrol, lactilol, isomalt, maltilol, lactose, fructose, maltodextrin, dextrin, and inverted sugar are substances generally regarded as safe for human consumption. In conclusion, according to the currently available evidence, aspartame, sucralose, acessulfame-K and stevia can be safely used during pregnancy.

  13. Avaliação sensorial de bebidas de goiaba adoçadas com diferentes agentes adoçantes Sensory evaluation of guava drinks sweetened with different sweetening agents

    Directory of Open Access Journals (Sweden)

    Aline Gurgel Fernandes

    2009-06-01

    Full Text Available Devido à crescente procura por bebidas à base de frutas tropicais e à expansão do segmento de produtos com valores calóricos reduzidos, este trabalho objetivou avaliar o grau de doçura, sabor, impressão global e a intenção de compra das bebidas de goiaba adoçadas com diferentes agentes adoçantes através de frequência de notas, teste de médias e Mapa de Preferência Interno (MPI. Utilizaram-se polpa de goiaba, água potável e diferentes agentes adoçantes (sacarose, mistura ciclamato/sacarina, aspartame, acesulfame-K, estévia e mistura sacarina/ciclamato sódico/acesulfame-K. A avaliação sensorial foi realizada com 100 provadores não treinados em teste laboratorial através do delineamento de blocos completos balanceados. Objetivando a avaliação das respostas individuais de cada provador, as respostas sensoriais foram avaliadas pela metodologia do MPI, empregando-se a técnica de Análise de Componentes Principais. As bebidas de goiaba adoçadas com sacarose e aspartame apresentaram avaliação sensorial semelhante por parte dos consumidores, apresentando os maiores valores médios no teste de aceitação, enquanto as adoçadas com estévia, com a mistura de três agentes adoçantes e a mistura ciclamato/sacarina apresentaram os valores mais baixos na avaliação sensorial. A utilização do MPI confirmou os resultados obtidos através de frequência de notas e teste de médias.Due to a constant search for tropical fruit drinks and calorie reduced products, this work aimed to evaluate the degree of sweetness, flavor, overall global impression, and purchase intention of guava drinks sweetened with different sweetening agents through the response rank frequency and average test, and internal preference mapping (IPM. Guava pulp, drinking water, and sweetening agents (sucrose, mixture cyclamate/saccharine, aspartame, acesulfame K, stevia and mixture saccharine/sodium cyclamate/acesulfame K were used. The sensory evaluation was

  14. Determination of storage conditions for new biscuits using their sorption isotherms

    Directory of Open Access Journals (Sweden)

    G. Diukareva

    2015-05-01

    Full Text Available Introduction For the formation of biscuits quality natural carrier of iodine and sweetener from stevia leaves were used. Desorption of moisture is the dominant process, which will determine the guaranteed shelf life of biscuits. The conditions for the developed biscuits storage was determined by investigating of sorption isotherms and kinetics of reaching the equilibrium moisture content. Materials and Methods. The objects of study arenewly developed biscuits: "Health" (rich in iodine and with the replacement of 50 % sugar by stevioside, "Light" (with the replacement of 75% of sugar by stevioside and enriched with wheat bran, "Fortified" (containselamine, which is a natural source of iodine. Control – the biscuit prepared according to traditional recipes. Tenzometric method was used to study the sorption equilibrium moisture content. Differential function of pore radius distribution was determined using sorption isotherms and then have been subjected to approximation. Results. New biscuits samples are in the area of polymolecular and monomolecular sorption in the range of the relative air humidity (RAH from 10 to 75 ... 80%. The control sample has less distinct plot of monomolecular sorption (10 to 20% and short- moisture range wich is corresponding with polymolecularsorption (from 20 to 65 ... 70%. There is a moisture absorbtion ofmicrocapillary and swelling of samples when RAHincreases to 75 ... 80% for all the samples. The ratio of average pore radius tothe most likely of the test samples are differentthat wasshown by the investigation of differential function of pore distribution. So this ratio for biscuit "Health" is 5.73, for biscuit "Light" − 2.98,forbiscuit "Fortified"− 4.91 and for the control − 3.88. Conclusions. There’s the sense to store developed biscuits in a cardboard packaging with polymeric covering, if RAH is not more than 75%, and vapor-proof if RAH is above that.

  15. Position of the Academy of Nutrition and Dietetics: use of nutritive and nonnutritive sweeteners.

    Science.gov (United States)

    Fitch, Cindy; Keim, Kathryn S

    2012-05-01

    It is the position of the Academy of Nutrition and Dietetics that consumers can safely enjoy a range of nutritive sweeteners and nonnutritive sweeteners (NNS) when consumed within an eating plan that is guided by current federal nutrition recommendations, such as the Dietary Guidelines for Americans and the Dietary Reference Intakes, as well as individual health goals and personal preference. A preference for sweet taste is innate and sweeteners can increase the pleasure of eating. Nutritive sweeteners contain carbohydrate and provide energy. They occur naturally in foods or may be added in food processing or by consumers before consumption. Higher intake of added sugars is associated with higher energy intake and lower diet quality, which can increase the risk for obesity, prediabetes, type 2 diabetes, and cardiovascular disease. On average, adults in the United States consume 14.6% of energy from added sugars. Polyols (also referred to as sugar alcohols) add sweetness with less energy and may reduce risk for dental caries. Foods containing polyols and/or no added sugars can, within food labeling guidelines, be labeled as sugar-free. NNS are those that sweeten with minimal or no carbohydrate or energy. They are regulated by the Food and Drug Administration as food additives or generally recognized as safe. The Food and Drug Administration approval process includes determination of probable intake, cumulative effect from all uses, and toxicology studies in animals. Seven NNS are approved for use in the United States: acesulfame K, aspartame, luo han guo fruit extract, neotame, saccharin, stevia, and sucralose. They have different functional properties that may affect perceived taste or use in different food applications. All NNS approved for use in the United States are determined to be safe. Copyright © 2012 Academy of Nutrition and Dietetics. Published by Elsevier Inc. All rights reserved.

  16. Evaluation of Brazilian light ketchups II: quantitative descriptive and physicochemical analysis Avaliação de catchups light do mercado brasileiro II: análise descritiva quantitativa e avaliação físico-química

    Directory of Open Access Journals (Sweden)

    Gisele Cristina Maziero de Campos Bannwart

    2008-03-01

    Full Text Available Samples of ketchup available on the Brazilian market, one traditional (sweetened with sucrose and three light versions (sweetened with aspartame, acesulfame-K and a blend of cyclamate, saccharin and stevia were evaluated for their physicochemical characteristics and sensory profile (Quantitative Descriptive Analysis. Four main groups of attributes were generated: appearance, oral texture, aroma and flavor. The samples presented significant differences in all attributes, except for syneresis and overripe tomato flavor. The highest means for sweetener and bitter tastes and aftertastes were observed for the samples sweetened with acesulfame-K and the blend of sweeteners. Although different characteristics were observed among the products evaluated and, despite the differences in the formulations, the light ketchup sweetened with aspartame was the one that presented properties most similar to those of the traditional ketchup.Amostras de catchup disponíveis no mercado brasileiro, uma tradicional (adoçada com sacarose e três light (adoçadas com aspartame, acessulfame-K e uma combinação de ciclamato, sacarina e estévia foram avaliadas quanto às características físico-químicas e ao perfil sensorial (Análise Descritiva Quantitativa. Quatro grandes grupos de atributos foram gerados: aparência, textura bucal, aroma e sabor. As amostras apresentaram diferença significativa em todos os atributos, exceto sinerese e tomate passado. As maiores médias para os atributos relacionados a sabor e sabor residual amargo e de adoçante foram obtidas para as amostras adoçadas com acessulfame-K e com a combinação de edulcorantes. Apesar das diferentes características observadas entre os produtos avaliados e das diferenças entre as formulações, o catchup light adoçado com aspartame foi o que apresentou propriedades mais próximas ao produto tradicional.

  17. Low Caloric Sweeteners for Diabetes and Obesity Care and Their

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    Mohammad Asif

    2015-10-01

    Full Text Available Diabetes and obesity are two common human disorders that affecting human health and invite various diseases and disorders in normal body functions. These diseases are very common worldwide. Diabetes occurs when high blood sugar levels develop. This happens when body can’t make and use all of the insulin it needs to blood sugar normally to keep blood sugar levels as normal as possible to control diabetes. Diabetic patients will need to follow a diet plan, do exercise and possibly take insulin injections. As part of eating plan, health care provider, and dietitian may ask to limit the amount of carbohydrates eat each day. Low-calorie sweeteners are one easy tool to help for follow eating plan. Obesity is more susceptible and often been associated with frequent ingestion of high energy food in high amount and high intake of sugars such as fermentable sugars such as sucrose, fructose, glucose, and maltose. Both diseases are may be genetically or due to hormonal imbalances. High energy sweeteners may causes caries in the teeth particularly susceptible to the children. Increased calorie intake associated with sugars and carbohydrates, especially when associated with physical inactivity, has been implicated in obesity. Fortunately, low calorie artificial and natural alternatives of sugars have been developed as alternatives to fermentable sugars and have shown promise in these health issues. Although there are only few artificial sweeteners (saccharin, aspartame, acesulfam potassium, sucralose, cyclamate that have been approved as food additives by the Food and Drug Administration and additional other low-caloric sweeteners (sugar alcohols, neotame, stevia, erythritol, xylitol, tagatose that have FDA-generally recognized as safe. Given the health impact of sugars and other carbohydrates, professionals should be aware of the marketed available low caloric sweeteners and both their benefits and potential risks.

  18. Conocimiento de seis especies arbustivas utilizadas como plantas nodrizas en el occidente de Guatemala

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    José Vicente Martínez-Arévalo

    2015-11-01

    Full Text Available En la restauración de ecosistemas, una técnica es utilizar arbustos de la sucesión como nodriza. En la parte alta de San Marcos, Guatemala, se ha recurrido al menos a seis de ellos en el establecimiento de pino y pinabete: arrayán (Baccharis vaccinoides Kunth, salvia (Buddleia megalocephala Donn. Sm., mozote (Acaena elongata L., chicajol (Stevia polycephala Bertol, mora (Rubus trilobus Ser. y malacate (Symphoricarpos microphyllus Kunth. Se carece de la documentación de su comportamiento y características que puedan servir para utilizarlas ampliamente como plantas protectoras. En esta investigación se estudió características in situ y reproductivas de estas especies. Entre los principales resultados está que la altura de plantas es de 1.17 a 2.64 m y cobertura de 0.34 a 2.77 m2, con una amplia variación entre y dentro de especies. La cantidad de frutos/planta va desde 90 en S. microphyllus a 59,400 en B. vaccinioides. La propagación vegetativa en campo estuvo entre 48 a 92 %, mayor que cuando se realizó en vivero, donde B. vaccinoides, S. microphyllus y R. trilobus tuvieron mejor respuesta. La propagación por semilla muestra que las seis especies pueden reproducirse adecuadamente. Estos resultados permiten su reproducción en viveros para su uso en restauración ecológica.

  19. Isolation and optimization of pectinase enzyme production one of useful industrial enzyme in Aspergillus niger, Rhizopus oryzae, Penicilium chrysogenum

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    akram songol

    2016-06-01

    Full Text Available Introduction: Pectinase enzyme is one of the most important industrial enzymes which isolated from a wide variety of microorganisms such as bacteria and filamentous fungi. This enzyme has been usually used in the fruit and textile industry. In this study, the isolation and optimization of pectinase-producing fungi on decaying rotten fruits were studied. Materials and methods: Isolation and screening of pectinase producing fungi performed through plate culture on pectin medium and staining with Lugol's iodine solution. The best strains were identified by ITS1, 4 sequencing as Aspergillus fumigatus, Rhizopus oryzae, Penicilium chrysogenum. The enzyme production was optimized by application of the five factorial design, each at three levels. These factors are carbon sources (whey, glucose and stevia, ammonium sulfate, manganese sulfate, temperature, and pH. Pectinase concentration was measured by the Miller method. Results: The results indicate that optimum condition for enzyme production for three fungi strains was obtained at 32 °C, pH = 6, 3g / L manganese sulfate, 2.75g / L of ammonium sulfate and 10g / L of each carbon source. The best experiment in obtaining the optimum enzyme contained 1.328 mg / ml of glucose for Aspergillus niger 1.284 and 1.039 mg / ml of whey for Rhizopus oryzae and Penicilium chrysogenum. Molecular weight of enzyme was about 40 and 37 kDa which was obtained by SDS- PAGE. Discussion and conclusion: The results indicate that three strains could grow in a wide range of carbon source, pH and temperature, which could be a good candidate for industrial application.

  20. Averting comfortable lifestyle crises.

    Science.gov (United States)

    Bilton, Rod

    2013-01-01

    : alternative non-sugar sweeteners; toxic side-effects of aspartame. Stevia and xylitol as healthy sugar replacements; the role of food processing in dietary health; and beneficial effects of resistant starch in natural and processed foods. The rise of maize and soya-based vegetable oils have led to omega-6 fat overload and imbalance in the dietary ratio of omega-3 to omega-6 fats. This has led to toxicity studies with industrial trans fats; investigations on health risks associated with stress and comfort eating; and abdominal obesity. Other factors to consider are: diet, cholesterol and oxidative stress, as well as the new approaches to the chronology of eating and the health benefits of intermittent fasting.