WorldWideScience

Sample records for eso 243-49 hlx-1

  1. Understanding the environment around the intermediate mass black hole candidate ESO 243-49 HLX-1

    Science.gov (United States)

    Webb, N. A.; Guérou, A.; Ciambur, B.; Detoeuf, A.; Coriat, M.; Godet, O.; Barret, D.; Combes, F.; Contini, T.; Graham, Alister W.; Maccarone, T. J.; Mrkalj, M.; Servillat, M.; Schroetter, I.; Wiersema, K.

    2017-06-01

    Aims: ESO 243-49 HLX-1, otherwise known as HLX-1, is an intermediate mass black hole (IMBH) candidate located 8'' (3.7 Kpc) from the centre of the edge-on S0 galaxy ESO 243-49. How the black hole came to be associated with this galaxy, and the nature of the environment in which it resides, remain unclear. Using multi-wavelength observations we aim to investigate the nature of the medium surrounding HLX-1, search for evidence of past mergers with ESO 243-49 and constrain parameters of the galaxy, including the mass of the expected central supermassive black hole, essential for future modelling of the interaction of the IMBH and ESO 243-49. Methods: We have reduced and analysed integral field unit observations of ESO 243-49 that were taken with the MUSE instrument on the VLT. Using complementary multi-wavelength data, including X-shooter, HST, Swift, Chandra and ATCA data, we have further examined the vicinity of HLX-1. We additionally examined the nature of the host galaxy and estimate the mass of the central supermassive black hole in ESO 243-49 using (black hole mass)-(host spheroid) scaling relations and the fundamental plane of black hole activity. Results: No evidence for a recent minor-merger that could result in the presence of the IMBH is discerned, but the data are compatible with a scenario in which minor mergers may have occurred in the history of ESO 243-49. The MUSE data reveal a rapidly rotating disc in the centre of the galaxy, around the supermassive black hole. The mass of the supermassive black hole at the centre of ESO 243-49 is estimated to be 0.5-23 × 107M⊙. Studying the spectra of HLX-1, that were taken in the low and hard state, we determine Hα flux variability to be at least a factor 6, compared to observations taken during the high and soft state. This Hα flux variability over one year indicates that the line originates close to the intermediate mass black hole, excluding the possibility that the line emanates from a surrounding nebula

  2. Investigating SLIM Disk Solutions FOR HLX-1 IN ESO 243-49

    Science.gov (United States)

    Godet, O.; Plazolles, B.; Kawaguchi, T.; Lasota, J.-P; Barret, d.; Farrell, S. A.; Braito, V.; Servillat, M.; Webb, N.; Gehrels, N.

    2012-01-01

    The hyperluminous X-ray source HLX-1 in the galaxy ESO 243-49, currently the best intermediate-mass blackhole (BH) candidate, displays spectral transitions similar to those observed in Galactic BH binaries, but with aluminosity 100-1000 times higher. We investigated the X-ray properties of this unique source by fitting multiepochdata collected by Swift, XMM-Newton, and Chandra with a disk model computing spectra for a wide rangeof sub- and super-Eddington accretion rates assuming a non-spinning BH and a face-on disk (i=0 deg.). Under theseassumptions we find that the BH in HLX-1 is in the intermediate-mass range (approximately 2 x 10(exp 4) solar mass) and the accretionflow is in the sub-Eddington regime. The disk radiation efficiency is eta = 0.11 plus or minus 0.03. We also show that the source does follow the LX is proportional to T(exp 4) relation for our mass estimate. At the outburst peaks, the source radiates near the Eddington limit. The accretion rate then stays constant around 4 x 10(exp 4) solar mass yr (sup -1) for several days and then decreases exponentially. Such plateaus in the accretion rate could be evidence that enhanced mass-transfer rateis the driving outburst mechanism in HLX-1. We also report on the new outburst observed in 2011 August by theSwift X-Ray Telescope. The time of this new outburst further strengthens the approximately 1 year recurrence timescale.

  3. A YOUNG MASSIVE STELLAR POPULATION AROUND THE INTERMEDIATE-MASS BLACK HOLE ESO 243-49 HLX-1

    International Nuclear Information System (INIS)

    Farrell, S. A.; Servillat, M.; Pforr, J.; Maraston, C.; Maccarone, T. J.; Knigge, C.; Godet, O.; Webb, N. A.; Barret, D.; Belmont, R.; Gosling, A. J.; Wiersema, K.

    2012-01-01

    We present Hubble Space Telescope and simultaneous Swift X-ray Telescope observations of the strongest candidate intermediate-mass black hole (IMBH) ESO 243-49 HLX-1. Fitting the spectral energy distribution from X-ray to near-infrared wavelengths showed that the broadband spectrum is not consistent with simple and irradiated disk models, but is well described by a model comprised of an irradiated accretion disk plus a ∼10 6 M ☉ stellar population. The age of the population cannot be uniquely constrained, with both young and old stellar populations allowed. However, the old solution requires excessive disk reprocessing and an extremely small disk, so we favor the young solution (∼13 Myr). In addition, the presence of dust lanes and the lack of any nuclear activity from X-ray observations of the host galaxy suggest that a gas-rich minor merger may have taken place less than ∼200 Myr ago. Such a merger event would explain the presence of the IMBH and the young stellar population.

  4. Radio Detections During Two State Transitions of the Intermediate-Mass Black Hole HLX-1

    Science.gov (United States)

    Webb, Natalie; Cseh, David; Lenc, Emil; Godet, Olivier; Barret, Didier; Corbel, Stephane; Farrell, Sean; Fender, Robert; Gehrels, Neil; Heywood, Ian

    2012-01-01

    Relativistic jets are streams of plasma moving at appreciable fractions of the speed of light. They have been observed from stellar-mass black holes (approx. 3 to 20 solar masses) as well as supermassive black holes (approx.. 10(exp 6) to 10(exp 9) Solar Mass) found in the centers of most galaxies. Jets should also be produced by intermediate-mass black holes (approx. 10(exp 2) to 10(exp 5) Solar Mass), although evidence for this third class of black hole has, until recently, been weak. We report the detection of transient radio emission at the location of the intermediate-mass black hole candidate ESO 243-49 HLX-1, which is consistent with a discrete jet ejection event. These observations also allow us to refine the mass estimate of the black hole to be between approx. 9 × 10(exp 3) Solar Mass and approx. 9 × 10(exp 4) Solar Mass.

  5. Radio detections during two state transitions of the intermediate-mass black hole HLX-1.

    Science.gov (United States)

    Webb, Natalie; Cseh, David; Lenc, Emil; Godet, Olivier; Barret, Didier; Corbel, Stephane; Farrell, Sean; Fender, Robert; Gehrels, Neil; Heywood, Ian

    2012-08-03

    Relativistic jets are streams of plasma moving at appreciable fractions of the speed of light. They have been observed from stellar-mass black holes (~3 to 20 solar masses, M(⊙)) as well as supermassive black holes (~10(6) to 10(9) M(⊙)) found in the centers of most galaxies. Jets should also be produced by intermediate-mass black holes (~10(2) to 10(5) M(⊙)), although evidence for this third class of black hole has, until recently, been weak. We report the detection of transient radio emission at the location of the intermediate-mass black hole candidate ESO 243-49 HLX-1, which is consistent with a discrete jet ejection event. These observations also allow us to refine the mass estimate of the black hole to be between ~9 × 10(3) M(⊙) and ~9 × 10(4) M(⊙).

  6. Age and mass of the star cluster around the intermediate-mass black hole HLX-1

    Science.gov (United States)

    Soria, Roberto

    2017-08-01

    We propose to study the optical counterpart of the intermediate-mass black hole (IMBH) HLX-1, about 3 years after its last X-ray outburst. Previous HST observations taken at epochs closer to an outburst show a variable near-UV/blue component, plus a constant red/near-IR component. The redder component probably comes from an old stellar population around the black hole; the bluer component may be a combination of emission from an irradiated accretion disk, and from a young stellar population. Their relative contributions and the age of the stellar population(s) are still subject of debate. By re-observing the system when the irradiated component is minimal, we will determine whether the near-UV/blue optical emission has declined even further and whether the red/near-IR optical emission has stayed approximately constant. This will tell us whether the optical counterpart is a massive star cluster, and place a strong upper limit to the young stellar population around the IMBH. Having determined the constant contribution of the stellar emission, we will then insert it into broad-band models of the emission in outburst, and obtain a better estimate of the accretion disk parameters, constraining formation and evolution scenarios for the IMBH. We will use the same set of filters as in previous HST observations, for direct comparison of brightness and colors: F140LP (ACS SBC); F300X, F336W, F390W, F555W, F621M, F775W (WFC3 UVIS); F105W, F160W (WFC3 IR). We request a total of 7 orbits.

  7. ESO Highlights in 2008

    Science.gov (United States)

    2009-01-01

    As is now the tradition, the European Southern Observatory looks back at the exciting moments of last year. 2008 was in several aspects an exceptionally good year. Over the year, ESO's telescopes provided data for more than 700 scientific publications in refereed journals, making ESO the most productive ground-based observatory in the world. ESO PR Highlights 2008 ESO PR Photo 01a/09 The image above is a clickable map. These are only some of the press releases issued by ESO in 2008. For a full listing, please go to ESO 2008 page. Austria signed the agreement to join the other 13 ESO member states (ESO 11/08 and 20/08), while the year marked the 10th anniversary of first light for ESO's "perfect science machine", the Very Large Telescope (ESO 16/08 and 17/08). The ALMA project, for which ESO is the European partner, had a major milestone in December, as the observatory was equipped with its first antenna (ESO 49/08). Also the Atacama Pathfinder Experiment (APEX) telescope impressed this year with some very impressive and publicly visible results. Highlights came in many fields: Astronomers for instance used the Very Large Telescope (VLT) to discover and image a probable giant planet long sought around the star Beta Pictoris (ESO 42/08). This is now the eighth extrasolar planet to have been imaged since the VLT imaged the first extrasolar planet in 2004 (three of eight were imaged with VLT). The VLT also enabled three students to confirm the nature of a unique planet (ESO 45/08). This extraordinary find, which turned up during their research project, is a planet about five times as massive as Jupiter. This is the first planet discovered orbiting a fast-rotating hot star. The world's foremost planet-hunting instrument, HARPS, located at ESO's La Silla observatory, scored a new first, finding a system of three super-Earths around a star (ESO 19/08). Based on the complete HARPS sample, astronomers now think that one Sun-like star out of three harbours short orbit, low

  8. Eso's Situation in Chile

    Science.gov (United States)

    1995-02-01

    ESO, the European Southern Observatory, in reply to questions raised by the international media, as well as an ongoing debate about the so-called "Paranal case" in Chilean newspapers, would like to make a number of related observations concerning its status and continued operation in that country [1]. THE ESO OBSERVATORY SITES IN CHILE The European Southern Observatory, an international organisation established and supported by eight European countries, has been operating more than 30 years in the Republic of Chile. Here ESO maintains one of the world's prime astronomical observatories on the La Silla mountain in the southern part of the Atacama desert. This location is in the Fourth Chilean Region, some 600 km north of Santiago de Chile. In order to protect the La Silla site against dust and light pollution from possible future mining industries, roads and settlements, ESO early acquired the territory around this site. It totals about 825 sq. km and has effectively contributed to the preservation of its continued, excellent "astronomical" quality. Each year, more than 500 astronomers from European countries, Chile and elsewhere profit from this when they come to La Silla to observe with one or more of the 15 telescopes now located there. In 1987, the ESO Council [2] decided to embark upon one of the most prestigious and technologically advanced projects ever conceived in astronomy, the Very Large Telescope (VLT). It will consist of four interconnected 8.2-metre telescopes and will become the largest optical telescope in the world when it is ready. It is safe to predict that many exciting discoveries will be made with this instrument, and it will undoubtedly play a very important role in our exploration of the distant universe and its many mysteries during the coming decades. THE VLT AND PARANAL In order to find the best site for the VLT, ESO performed a thorough investigation of many possible mountain tops, both near La Silla and in Northern Chile. They showed

  9. ESO: The CERN Years

    CERN Multimedia

    Schaeffer, A

    2012-01-01

    In 1970, CERN and ESO signed a collaboration agreement for the construction of the Observatory’s first telescope. That same year, ESO’s Telescope Division and Sky Atlas laboratory settled on the CERN site in Meyrin. Let’s turn back to the beginnings of this lasting and fruitful alliance.

  10. ESO's Two Observatories Merge

    Science.gov (United States)

    2005-02-01

    On February 1, 2005, the European Southern Observatory (ESO) has merged its two observatories, La Silla and Paranal, into one. This move will help Europe's prime organisation for astronomy to better manage its many and diverse projects by deploying available resources more efficiently where and when they are needed. The merged observatory will be known as the La Silla Paranal Observatory. Catherine Cesarsky, ESO's Director General, comments the new development: "The merging, which was planned during the past year with the deep involvement of all the staff, has created unified maintenance and engineering (including software, mechanics, electronics and optics) departments across the two sites, further increasing the already very high efficiency of our telescopes. It is my great pleasure to commend the excellent work of Jorge Melnick, former director of the La Silla Observatory, and of Roberto Gilmozzi, the director of Paranal." ESO's headquarters are located in Garching, in the vicinity of Munich (Bavaria, Germany), and this intergovernmental organisation has established itself as a world-leader in astronomy. Created in 1962, ESO is now supported by eleven member states (Belgium, Denmark, Finland, France, Germany, Italy, The Netherlands, Portugal, Sweden, Switzerland, and the United Kingdom). It operates major telescopes on two remote sites, all located in Chile: La Silla, about 600 km north of Santiago and at an altitude of 2400m; Paranal, a 2600m high mountain in the Atacama Desert 120 km south of the coastal city of Antofagasta. Most recently, ESO has started the construction of an observatory at Chajnantor, a 5000m high site, also in the Atacama Desert. La Silla, north of the town of La Serena, has been the bastion of the organization's facilities since 1964. It is the site of two of the most productive 4-m class telescopes in the world, the New Technology Telescope (NTT) - the first major telescope equipped with active optics - and the 3.6-m, which hosts HARPS

  11. 1970: ESO arrives at CERN

    CERN Multimedia

    Anaïs Schaeffer

    2012-01-01

    In 1970, CERN and ESO signed a collaboration agreement for the construction of the Observatory’s first telescope. That same year, ESO’s Telescope Division and Sky Atlas laboratory settled on the CERN site in Meyrin. Let’s turn back to the beginnings of this lasting and fruitful alliance.   Martin Cullum, ESO physicist from 1973 to 2009 In 1974, when I attended my first international conference on behalf of ESO, the first thing people asked was: “Where are you from?” “ESO,” I would say. The response: ‘'What on Earth is ESO?” Nowadays, people I meet seem to know more about ESO than I do! It is clear that ESO profited enormously from its sojourn at CERN. The wise decision of the ESO Director General at the time, Adriaan Blaauw, to move to Geneva allowed ESO to build up its technical expertise to be able to build world-beating projects like the VLT, which has undoubtedly been the most exciting scientif...

  12. Relations Between Chile and ESO

    Science.gov (United States)

    1994-06-01

    As announced in an earlier Press Release (PR 08/94 of 6 May 1994), a high-ranking ESO delegation visited Santiago de Chile during the week of 24 - 28 May 1994 to discuss various important matters of mutual interest with the Chilean Government. It consisted of Dr. Peter Creola (President of ESO Council), Dr. Catherine Cesarsky (Vice-President of ESO Council), Dr. Henrik Grage (Former Vice-President of ESO Council) and Professor Riccardo Giacconi (ESO Director General), the latter accompanied by his advisers. THE SUPPLEMENTARY TREATY BETWEEN CHILE AND ESO Following a meeting with the ambassadors to Chile of the eight ESO member countries, the ESO delegation was received by the Chilean Minister of Foreign Affairs, Mr. Carlos Figueroa, and members of his staff. The ESO delegation was pleased to receive assurances that the present Chilean Government, like its predecessors, will continue to honour all contractual agreements, in particular the privileges and immunities of this Organisation, which were laid down in the Treaty between ESO and Chile that was signed by the parties in 1963 and ratified the following year. The discussions covered some aspects of the proposed Supplementary Treaty which has been under preparation during the past year. This included in particular the desire of the Chilean side to further increase the percentage of guaranteed time for Chilean astronomers at the future ESO Very Large Telescope (VLT) and also the rules governing the installation by ESO member countries of additional telescopes at the ESO observatories in Chile. ESO invited a Chilean delegation to visit the ESO Headquarters in Garching (Germany) later this year for the final adjustment of the text of the Supplementary Treaty, after which it should be possible to proceed rapidly with the signing and ratification by the Chilean Parliament and the ESO Council. THE SITUATION AROUND PARANAL The ESO delegation expressed its deep concern to the Chilean Government about the continuing legal

  13. Portugal to Accede to ESO

    Science.gov (United States)

    2000-06-01

    The Republic of Portugal will become the ninth member state of the European Southern Observatory (ESO) [1]. Today, during a ceremony at the ESO Headquarters in Garching (Germany), a corresponding Agreement was signed by the Portuguese Minister of Science and Technology, José Mariano Gago and the ESO Director General, Catherine Cesarsky , in the presence of other high officials from Portugal and the ESO member states (see Video Clip 05/00 below). Following subsequent ratification by the Portuguese Parliament of the ESO Convention and the associated protocols [2], it is foreseen that Portugal will formally join this organisation on January 1, 2001. Uniting European Astronomy ESO PR Photo 16/00 ESO PR Photo 16/00 [Preview - JPEG: 400 x 405 pix - 160k] [Normal - JPEG: 800 x 809 pix - 408k] Caption : Signing of the Portugal-ESO Agreement on June 27, 2000, at the ESO Headquarters in Garching (Germany). At the table, the ESO Director General, Catherine Cesarsky , and the Portuguese Minister of Science and Technology, José Mariano Gago . In his speech, the Portuguese Minister of Science and Technology, José Mariano Gago , stated that "the accession of Portugal to ESO is the result of a joint effort by ESO and Portugal during the last ten years. It was made possible by the rapid Portuguese scientific development and by the growth and internationalisation of its scientific community." He continued: "Portugal is fully committed to European scientific and technological development. We will devote our best efforts to the success of ESO". Catherine Cesarsky , ESO Director General since 1999, warmly welcomed the Portuguese intention to join ESO. "With the accession of their country to ESO, Portuguese astronomers will have great opportunities for working on research programmes at the frontiers of modern astrophysics." "This is indeed a good time to join ESO", she added. "The four 8.2-m VLT Unit Telescopes with their many first-class instruments are nearly ready, and the VLT

  14. Trayectorias modelizadoras en la ESO

    Directory of Open Access Journals (Sweden)

    Manel Sol

    2011-06-01

    Full Text Available Se presentan los resultados de una investigación sobre las competencias modelizadoras de alumnos de la ESO con dificultades de aprendizaje de las matemáticas. Para ello se analizan las memorias escritas de sus actividades de modelización que hemos denominado Proyectos Matemáticos Realísticos. En especial se estudian las acciones que realizan los alumnos en la ejecución de su proyecto. La secuencia de todas las acciones que llevan a cabo los escolares lo hemos denominado  ”Ruta Modelizadora”. Para su análisis hemos establecido una ruta hipotética que los alumnos de la ESO, como modelizadores no expertos, pueden llevar a cabo en un proceso de modelización completo. Esta ruta hipotética, formada por 16 acciones, la hemos utilizado como un instrumento de análisis. Los resultados de la investigación muestran que todos los alumnos son capaces de llevar a cabo sus proyectos matemáticos aunque se observan diferencias en los procesos que siguen.

  15. Overview of ESO Large Single Dish Study

    Science.gov (United States)

    Testi, Leonardo

    2018-01-01

    In this talk I will briefly summarize the motivation, methodology and outcome of the ESO Submm Single Dish Strategy WG. The WG was established by the ESO Director for Science and completed its work at the end of 2015. I will summarize the status of the report recommendations, which, among other things, led to the organization of the AtLAST workshop.

  16. The Strategic Partnership between ESO and Australia

    Science.gov (United States)

    Comendador Frutos, L.; de Zeeuw, T..; Geeraert, P.

    2017-09-01

    On 11 July 2017, ESO and the Australian government signed a ten-year Strategic Partnership arrangement giving Australian astronomers access to the La Silla Paranal facilities. The path towards this arrangement is briefly outlined and the details of the Partnership and its implications for both the Australian and ESO astronomical communities are summarised.

  17. Current Discussions Between ESO and Chile

    Science.gov (United States)

    1995-04-01

    [Joint Press Release by the Government of the Republic of Chile and the European Southern Observatory. The text is issued simultaneously in Santiago de Chile (in Spanish) and at the ESO Headquarters in Garching (in English).] Today, Tuesday, 18 April 1995, at the ESO Headquarters in Garching (Germany), Mr. Roberto Cifuentes, Plenipotentiary Ambassador representing the Government of the Republic of Chile, and the Director General of the European Southern Observatory, Professor Riccardo Giacconi, have signed a Supplementary, Interpretative and Amending Agreement to the Convention of 6 November 1963 which governs the relations between Chile and this International Organisation. This Agreement which in practice signifies a widening and strengthening of the cooperative relations between the Organisation and the Chilean scientific community will hereafter be submitted for ratification by the National Congress of the Republic of Chile (the Parliament) and by the ESO Council. According to the Agreement signed today, Chilean astronomers will have privileged access within up to 10 percent observing time on all present and future ESO telescopes in Chile. Moreover, ESO accepts to incorporate into its labour regulations for Chilean personnel concepts like freedom of association and collective bargaining. This signing of the Supplementary, Interpretative and Amending Agreement to the original Convention of 1963 follows after months of constructive dialogue between the parties. It constitutes an important step towards a solution of some of the pending points on the current agenda for discussions between the Government of Chile and ESO. Among the issues still pending, ESO has informed the Government of Chile that respect for its immunities by the Chilean State is of vital importance for the continuation of the construction of the world's largest telescope at Paranal, as well as the continued presence of the Organisation in Chile. The Chilean Government, on its side, and concerning

  18. Urine antibody against human cancer antigen NY-ESO-1

    OpenAIRE

    Jäger, Dirk; Stockert, Elisabeth; Karbach, Julia; Herrlinger, Kristina; Atmaca, Akin; Arand, Michael; Chen, Yao-Tseng; Gnjatic, Sacha; Old, Lloyd J; Knuth, Alexander; Jäger, Elke

    2002-01-01

    NY-ESO-1 is one of the most immunogenic tumor antigens known to date. Spontaneous humoral and cellular immune responses against NY-ESO-1 are detected in a substantial proportion of patients with NY-ESO-1 positive cancers. NY-ESO-1 serum antibody is dependent on the presence of NY-ESO-1+ cancer cells, and antibody titers correlate with the clinical development of the disease. NY-ESO-1 serum antibody is associated with detectable NY-ESO-1-specific CD8+ T cell reactivity. High titers of NY-ESO-1...

  19. The Gaia-ESO Public Spectroscopic Survey

    OpenAIRE

    Gilmore, G.; Randich, S.; Asplund, M.; Binney, J.; Bonifacio, P.; Drew, J.; Feltzing, S.; Ferguson, A.; Jeffries, R.; Micela, G.; Negueruela, I.; Prusti, T.; Rix, H.-W.; Vallenari, A.; Alfaro, E.

    2012-01-01

    The Gaia-ESO Public Spectroscopic Survey has begun and will obtain high quality spectroscopy of some 100000 Milky Way stars, in the field and in open clusters, down to magnitude 19, systematically covering all the major components of the Milky Way. This survey will provide the first homogeneous overview of the distributions of kinematics and chemical element abundances in the Galaxy. The motivation, organisation and implementation of the Gaia-ESO Survey are described, emphasising the compleme...

  20. Czech Republic to Become Member of ESO

    Science.gov (United States)

    2006-12-01

    Today, an agreement was signed in Prague between ESO and the Czech Republic, aiming to make the latter become a full member of ESO as of 1 January 2007. "The future membership of the Czech Republic in ESO opens for the Czech astronomers completely new opportunities and possibilities. It will foster this discipline on the highest quality level and open new opportunities for Czech industry to actively cooperate in research and development of high-tech instruments for astronomical research," said Miroslava Kopicová, Minister of Education, Youth and Sports of the Czech Republic. ESO PR Photo 52/06 ESO PR Photo 52/06 Signing Ceremony "We warmly welcome the Czech Republic as the thirteenth member of ESO," said Catherine Cesarsky, ESO's Director General. "The timing couldn't be better chosen: with the Very Large Telescope, Europe is now at the forefront of ground-based astronomy, and with the construction of ALMA and the final studies for the European Extremely Large Telescope, we will ensure that this will remain so for several decades. We look forward to working together with our Czech colleagues towards these successes." The signing event took place at the Czech Ministry of Education, Youth and Sports in Prague. Following ratification by the Czech Parliament, the Czech Republic with thus join the twelve present member states of ESO, the European Organisation for Astronomical Research in the Southern Hemisphere: Belgium, Denmark, France, Finland, Germany, Italy, the Netherlands, Portugal, Spain, Sweden, Switzerland and the United Kingdom. The Czech Republic is the first country from Central and Eastern Europe to join ESO. Astronomy in the Czech Republic has a very long tradition that dates from as far back as 3500 BC. Four centuries ago, Tycho Brahe and Johannes Kepler established themselves in Prague at the invitation of the emperor Rudolph II, laying the ground for the first golden age in astronomy. Later, eminent scientists such as Christian Doppler, Ernst Mach and

  1. The 2007 ESO Instrument Calibration Workshop

    CERN Document Server

    Kaufer, Andreas; ESO Workshop

    2008-01-01

    The 2007 ESO Instrument Calibration workshop brought together more than 120 participants with the objective to a) foster the sharing of information, experience and techniques between observers, instrument developers and instrument operation teams, b) review the actual precision and limitations of the applied instrument calibration plans, and c) collect the current and future requirements by the ESO users. These present proceedings include the majority of the workshop’s contributions and document the status quo of instrument calibration at ESO in large detail. Topics covered are: Optical Spectro-Imagers, Optical Multi-Object Spectrographs, NIR and MIR Spectro-Imagers, High-Resolution Spectrographs, Integral Field Spectrographs, Adaptive Optics Instruments, Polarimetric Instruments, Wide Field Imagers, Interferometric Instruments as well as other crucial aspects such as data flow, quality control, data reduction software and atmospheric effects. It was stated in the workshop that "calibration is a life-long l...

  2. Catherine Cesarsky - ESO's Next Director General

    Science.gov (United States)

    1999-01-01

    The ESO Council has appointed Dr. Catherine Cesarsky as Director General for a five year period, succeeding Professor Riccardo Giacconi, whose term ends this year. Dr. Cesarsky will take up her duties on September 1, 1999, at the ESO Headquarters in Garching (Germany). Dr. Cesarsky was born in France in 1943. She received a degree in Physical Sciences at the University of Buenos Aires and graduated with a PhD in Astronomy in 1971 from Harvard University (Cambridge, Mass., USA). Afterwards she worked at the California Institute of Technology (CALTECH). In 1974, she became a staff member of the Service d'Astrophysique (SAp) , Direction des Sciences de la Matière (DSM), Commissariat à l'Energie Atomique (CEA) (France). She headed the theoretical group of the SAp (1978-1985), was Head of SAp (1985-1993) and has been Director of DSM since 1994. The DSM encompasses, at the CEA, activities of basic research in physics, chemistry, astrophysics and earth sciences and comprises about 3000 scientists, engineers, technicians, etc. Dr. Cesarsky is known for her successful research activities in several central areas of modern astrophysics. The first part of her career was devoted to the high-energy domain. This has involved studies of the propagation and composition of galactic cosmic rays, of matter and fields in the diffuse interstellar medium, as well as the acceleration of particles in astrophysical shocks, e.g. in connection with supernovae. She then turned to infrared astronomy. She is the Principal Investigator of the ISOCAM camera onboard the Infrared Space Observatory (ISO) of the European Space Agency (ESA) and, as such, she leads the ISOCAM central programme. It studies, in a coordinated way, the infrared emission from a variety of galactic and extragalactic sources and is yielding new and exciting results on star formation and galactic evolution. Dr. Cesarsky received the COSPAR (Committee on Space Research) Space Science Award in 1998. She is a member of many

  3. L'ESO au temps du CERN

    CERN Multimedia

    Schaeffer, A

    2012-01-01

    L’Observatoire européen austral (ESO) fête aujourd’hui ses 50 ans. Retour sur l’histoire d’un des plus grands laboratoires européens, qui fut soutenu et accompagné par le CERN dès ses premières heures.

  4. ESO Helps Antofagasta Region after the Earthquake

    Science.gov (United States)

    2007-11-01

    On November 14 at 12:41 local time, a major earthquake with magnitude 7.7 on the Richter scale affected the north of Chile. The epicentre was located 35 km from the city of Tocopilla and 170 km of Antofagasta. Two persons died and tens were injured, while buildings were damaged in several cities. In the Maria Elena-Tocopilla area, several thousand homes were destroyed or damaged. In an act of solidarity with the local community and its authorities, ESO immediately announced a donation of 30 millions Chilean pesos (around 40,000 euros) to Antofagasta's Regional Government to support reconstruction in the Region II. ESO and its staff have been shocked by the earthquake and its impact on local communities, especially on the people of Tocopilla. The ESO Representation in Chile formally contacted the regional authorities to explore with them possible ways to collaborate in this difficult moment. In addition, many of ESO staff are personally cooperating with the victims, under the coordination of Cruz Roja, the organisation currently in charge of implementing individual efforts.

  5. The Gaia-ESO Public Spectroscopic Survey

    DEFF Research Database (Denmark)

    Gilmore, G.; Randich, S.; Asplund, M.

    2012-01-01

    The Gaia-ESO Public Spectroscopic Survey has begun and will obtain high quality spectroscopy of some 100000 Milky Way stars, in the field and in open clusters, down to magnitude 19, systematically covering all the major components of the Milky Way. This survey will provide the first homogeneous...

  6. Unifying access to services: ESO's user portal

    Science.gov (United States)

    Chavan, A. M.; Tacconi-Garman, L. E.; Peron, M.; Sogni, F.; Canavan, T.; Nass, P.

    2006-06-01

    The European Southern Observatory (ESO) is in the process of creating a central access point for all services offered to its user community via the Web. That gateway, called the User Portal, will provide registered users with a personalized set of service access points, the actual set depending on each user's privileges. Correspondence between users and ESO will take place by way of "profiles", that is, contact information. Each user may have several active profiles, so that an investigator may choose, for instance, whether their data should be delivered to their own address or to a collaborator. To application developers, the portal will offer authentication and authorization services, either via database queries or an LDAP server. The User Portal is being developed as a Web application using Java-based technology, including servlets and JSPs.

  7. Deformable mirrors development program at ESO

    Science.gov (United States)

    Stroebele, Stefan; Vernet, Elise; Brinkmann, Martin; Jakob, Gerd; Lilley, Paul; Casali, Mark; Madec, Pierre-Yves; Kasper, Markus

    2016-07-01

    Over the last decade, adaptive optics has become essential in different fields of research including medicine and industrial applications. With this new need, the market of deformable mirrors has expanded a lot allowing new technologies and actuation principles to be developed. Several E-ELT instruments have identified the need for post focal deformable mirrors but with the increasing size of the telescopes the requirements on the deformable mirrors become more demanding. A simple scaling up of existing technologies from few hundred actuators to thousands of actuators will not be sufficient to satisfy the future needs of ESO. To bridge the gap between available deformable mirrors and the future needs for the E-ELT, ESO started a development program for deformable mirror technologies. The requirements and the path to get the deformable mirrors for post focal adaptive optics systems for the E-ELT is presented.

  8. Evolution of Optical Spectrograph Design at ESO

    Science.gov (United States)

    Dekker, Hans

    2009-06-01

    The evolution of optical spectrograph design and its implementation at ESO since 1980 is sketched out from the point of view of the instrumentation with which I have been closely involved. The instruments range from the early days of EFOSC, EMMI, UVES, GIRAFFE to the present-day X-shooter and important optical design features, such as the use of focal reducers and the white light pupil principle, are highlighted.

  9. ESO Catalogue Facility Design and Performance

    Science.gov (United States)

    Moins, C.; Retzlaff, J.; Arnaboldi, M.; Zampieri, S.; Delmotte, N.; Forchí, V.; Klein Gebbinck, M.; Lockhart, J.; Micol, A.; Vera Sequeiros, I.; Bierwirth, T.; Peron, M.; Romaniello, M.; Suchar, D.

    2013-10-01

    The ESO Phase 3 Catalogue Facility provides investigators with the possibility to ingest catalogues resulting from ESO public surveys and large programs and to query and download their content according to positional and non-positional criteria. It relies on a chain of tools that covers the complete workflow from submission to validation and ingestion into the ESO archive and catalogue repository and a web application to browse and query catalogues. This repository consists of two components. One is a Sybase ASE relational database where catalogue meta-data are stored. The second one is a Sybase IQ data warehouse where the content of each catalogue is ingested in a specific table that returns all records matching a user's query. Spatial indexing has been implemented in Sybase IQ to speed up positional queries and relies on the Spherical Geometry Toolkit from the Johns Hopkins University which implements the Hierarchical Triangular Mesh (HTM) algorithm. It is based on a recursive decomposition of the celestial sphere in spherical triangles and the assignment of an index to each of them. It has been complemented with the use of optimized indexes on the non-positional columns that are likely to be frequently used as query constraints. First tests performed on catalogues such as 2MASS have confirmed that this approach provides a very good level of performance and a smooth user experience that are likely to facilitate the scientific exploitation of catalogues.

  10. Blockbuster starring ESO Paranal opens tomorrow

    Science.gov (United States)

    2008-10-01

    The 22nd James Bond adventure is due for release tomorrow, 31 October 2008, in the UK and a week later in the rest of the world. A key location in the movie is the Residencia, the hotel for astronomers and staff at ESO's Paranal Observatory. Blockbuster starring ESO Paranal opens tomorrow ESO PR Photo 38/08 The James Bond "Quantum of Solace" filmmakers Quantum of Solace is the latest film in one of most successful movie franchises -- that of renowned 007 Agent James Bond of the British Secret Service MI6. The agent "on Her Majesty's secret service" is once again played by Daniel Craig. Key scenes of the movie were filmed at Paranal, the home of ESO's Very Large Telescope, and the most advanced optical telescope in the world. Usually occupied by no more than 100 astronomers, engineers and technicians, Paranal welcomed the 300-strong film crew for several days of shooting at the end of March 2008. The crew travelled from their hotel base in Antofagasta for up to two hours each morning to reach the filming locations. "We are delighted to have a movie like this filmed at Paranal and it was extremely good to see how careful the crew were with the surroundings and how mindful they were of the fact that they were in an operating, working observatory", says Tim de Zeeuw, ESO Director General. "Paranal is a unique observatory in a unique setting and it is no real surprise that it plays a major part in a James Bond movie", he adds. The filmmakers were mostly interested in filming exterior scenes at the Paranal Residencia, the accommodation for staff operating the Very Large Telescope. In the movie, the Residencia is supposedly the "Perla de Las Dunas", a unique hotel in the desert. Cerro Paranal is a 2600 m high mountain in the Chilean Atacama Desert, perhaps the driest on Earth. The high altitude site and extreme dryness make excellent conditions for astronomical observations. To make it possible for people to live and work here, a hotel, or Residencia, was built at the

  11. The AstroMundus-ESO Connection

    Science.gov (United States)

    Humphreys, E.; Hussain, G.; Biggs, A.; Lu, H.-Y.; Emsellem, E.; De Cia, A.; Lavail, A.; Spyromilio, J.

    2016-03-01

    The AstroMundus Programme is an E+ Erasmus Mundus Joint Masters Degree course in astronomy and astrophysics offered by a consortium of European universities and research institutes. In 2014 and 2016, AstroMundus Masters students visited ESO and participated in proposal-writing sessions, during which groups of students speed-wrote complete ALMA proposals, before presenting them to a pseudo Time Allocation Committee providing on-the-spot feedback. The AstroMundus visit of 25-26 January 2016 is described.

  12. ESO and NSF Sign Agreement on ALMA

    Science.gov (United States)

    2003-02-01

    Green Light for World's Most Powerful Radio Observatory On February 25, 2003, the European Southern Observatory (ESO) and the US National Science Foundation (NSF) are signing a historic agreement to construct and operate the world's largest and most powerful radio telescope, operating at millimeter and sub-millimeter wavelength. The Director General of ESO, Dr. Catherine Cesarsky, and the Director of the NSF, Dr. Rita Colwell, act for their respective organizations. Known as the Atacama Large Millimeter Array (ALMA), the future facility will encompass sixty-four interconnected 12-meter antennae at a unique, high-altitude site at Chajnantor in the Atacama region of northern Chile. ALMA is a joint project between Europe and North America. In Europe, ESO is leading on behalf of its ten member countries and Spain. In North America, the NSF also acts for the National Research Council of Canada and executes the project through the National Radio Astronomy Observatory (NRAO) operated by Associated Universities, Inc. (AUI). The conclusion of the ESO-NSF Agreement now gives the final green light for the ALMA project. The total cost of approximately 650 million Euro (or US Dollars) is shared equally between the two partners. Dr. Cesarsky is excited: "This agreement signifies the start of a great project of contemporary astronomy and astrophysics. Representing Europe, and in collaboration with many laboratories and institutes on this continent, we together look forward towards wonderful research projects. With ALMA we may learn how the earliest galaxies in the Universe really looked like, to mention but one of the many eagerly awaited opportunities with this marvellous facility". "With this agreement, we usher in a new age of research in astronomy" says Dr. Colwell. "By working together in this truly global partnership, the international astronomy community will be able to ensure the research capabilities needed to meet the long-term demands of our scientific enterprise, and

  13. The ESO-LV project - Automated parameter extraction for 16000 ESO/Uppsala galaxies

    NARCIS (Netherlands)

    Lauberts, Andris; Valentijn, Edwin A.

    1987-01-01

    A program to extract photometric and morphological parameters of the galaxies in the ESO/Uppsala survey (Lauberts and Valentijn, 1982) is discussed. The completeness and accuracy of the survey are evaluated and compared with other surveys. The parameters obtained in the program are listed.

  14. ESO and Chile: 10 Years of Productive Scientific Collaboration

    Science.gov (United States)

    2006-06-01

    ESO and the Government of Chile launched today the book "10 Years Exploring the Universe", written by the beneficiaries of the ESO-Chile Joint Committee. This annual fund provides grants for individual Chilean scientists, research infrastructures, scientific congresses, workshops for science teachers and astronomy outreach programmes for the public. In a ceremony held in Santiago on 19 June 2006, the European Organisation for Astronomical Research in the Southern Hemisphere (ESO) and the Chilean Ministry of Foreign Affairs marked the 10th Anniversary of the Supplementary Agreement, which granted to Chilean astronomers up to 10 percent of the total observing time on ESO telescopes. This agreement also established an annual fund for the development of astronomy, managed by the so-called "ESO-Chile Joint Committee". ESO PR Photo 21/06 ESO PR Photo 21/06 Ten Years ESO-Chile Agreement Ceremony The celebration event was hosted by ESO Director General, Dr. Catherine Cesarsky, and the Director of Special Policy for the Chilean Ministry of Foreign Affairs, Ambassador Luis Winter. "ESO's commitment is, and always will be, to promote astronomy and scientific knowledge in the country hosting our observatories", said ESO Director General, Dr. Catherine Cesarsky. "We hope Chile and Europe will continue with great achievements in this fascinating joint adventure, the exploration of the universe." On behalf of the Government of Chile, Ambassador Luis Winter outlined the historical importance of the Supplementary Agreement, ratified by the Chilean Congress in 1996. "Such is the magnitude of ESO-Chile Joint Committee that, only in 2005, this annual fund represented 8 percent of all financing sources for Chilean astronomy, including those from Government and universities", Ambassador Winter said. The ESO Representative and Head of Science in Chile, Dr. Felix Mirabel, and the appointed Chilean astronomer for the ESO-Chile Joint Committee, Dr. Leonardo Bronfman, also took part in the

  15. Recent developments in evolutionary structural optimization (ESO) for continuum structures

    Science.gov (United States)

    Xie, Yi Min; Huang, Xiaodong

    2010-06-01

    Evolutionary Structural Optimization (ESO) and its later version bi-directional ESO (BESO) have gained widespread popularity among researchers in structural optimization and practitioners in engineering and architecture. However, there have also been many critical comments on various aspects of ESO/BESO. To address those criticisms, we have carried out extensive work to improve the original ESO/BESO algorithms in recent years. This paper summarizes some of the latest developments in the BESO method for topology optimization of continuum structures. Numerical results show that the ESO/BESO solutions agree well with those of other well-established topology optimization methods. It indicates that the current BESO method has great potential to become a robust and efficient design tool for practical applications in engineering and architecture.

  16. Recent developments in evolutionary structural optimization (ESO) for continuum structures

    International Nuclear Information System (INIS)

    Xie Yimin; Huang Xiaodong

    2010-01-01

    Evolutionary Structural Optimization (ESO) and its later version bi-directional ESO (BESO) have gained widespread popularity among researchers in structural optimization and practitioners in engineering and architecture. However, there have also been many critical comments on various aspects of ESO/BESO. To address those criticisms, we have carried out extensive work to improve the original ESO/BESO algorithms in recent years. This paper summarizes some of the latest developments in the BESO method for topology optimization of continuum structures. Numerical results show that the ESO/BESO solutions agree well with those of other well-established topology optimization methods. It indicates that the current BESO method has great potential to become a robust and efficient design tool for practical applications in engineering and architecture.

  17. NY-ESO-1 specific antibody and cellular responses in melanoma patients primed with NY-ESO-1 protein in ISCOMATRIX and boosted with recombinant NY-ESO-1 fowlpox virus.

    Science.gov (United States)

    Chen, Ji-Li; Dawoodji, Amina; Tarlton, Andrea; Gnjatic, Sacha; Tajar, Abdelouahid; Karydis, Ioannis; Browning, Judy; Pratap, Sarah; Verfaille, Christian; Venhaus, Ralph R; Pan, Linda; Altman, Douglas G; Cebon, Jonathan S; Old, Lloyd L; Nathan, Paul; Ottensmeier, Christian; Middleton, Mark; Cerundolo, Vincenzo

    2015-03-15

    Vaccination strategies based on repeated injections of NY-ESO-1 protein formulated in ISCOMATRIX particles (NY-ESO-1 ISCOMATRIX) have shown to elicit combined NY-ESO-1 specific antibody and T cell responses. However, it remains unclear whether heterologous prime-boost strategies based on the combination with NY-ESO-1 ISCOMATRIX with different NY-ESO-1 boosting reagents could be used to increase NY-ESO-1 CD8(+) or CD4(+) T cell responses. To address this question, we carried out a randomized clinical trial in 39 high-risk, resected melanoma patients vaccinated with NY-ESO-1 ISCOMATRIX, and then boosted with repeated injections of either recombinant fowlpox virus encoding full length NY-ESO-1 (rF-NY-ESO-1) (Arm A) or NY-ESO-1 ISCOMATRIX alone (Arm B). We have comprehensively analyzed NY-ESO-1 specific T cells and B cells response in all patients before and after vaccination for a total of seven time points per patient. NY-ESO-1 ISCOMATRIX alone elicited a strong NY-ESO-1 specific CD4(+) T cell and antibody response, which was maintained by both regiments at similar levels. However, CD8(+) T cell responses were significantly boosted in 3 out of 18 patients in Arm A after the first rF-NY-ESO-1 injection and such responses were maintained until the end of the trial, while no patients in Arm B showed similar CD8(+) T cell responses. In addition, our results clearly identified immunodominant regions in the NY-ESO-1 protein: NY-ESO-179-102 and NY-ESO-1115-138 for CD4+ T cells and NY-ESO-185-108 for CD8+ T cells in a large proportion of vaccinated patients. These regions of NY-ESO-1 protein should be considered in future clinical trials as immunodominant epitopes. © 2014 UICC.

  18. Young Astronomers' Observe with ESO Telescopes

    Science.gov (United States)

    1995-11-01

    Today, forty 16-18 year old students and their teachers are concluding a one-week, educational `working visit' to the ESO Headquarters in Garching (See ESO Press Release 14/95 of 8 November 1995). They are the winners of the Europe-wide contest `Europe Towards the Stars', organised by ESO with the support of the European Union, under the auspices of the Third European Week for Scientific and Technological Culture. From November 14-20, they have worked with professional ESO astronomers in order to get insight into the methods and principles of modern astronomy and astrophysics, as carried out at one of the world's foremost international centres. This included very successful remote observations with the ESO 3.5-m New Technology Telescope (NTT) and the 1.4-m Coude Auxiliary Telescope (CAT) via a satellite link between the ESO Headquarters and the La Silla observatory in Chile, 12,000 kilometres away. After a general introduction to modern astronomy on the first day of the visit, the participants divided into six teams, according to their interests. Some chose to observe distant galaxies, others prefered to have a closer look on binary stars, and one team decided to investigate a star which is thought to be surrounded by a proto-planetary system. Each team was supported by an experienced ESO astronomer. Then followed the observations at the remote consoles during three nights, the first at the NTT and the following at the CAT. Each team had access to the telescope during half a night. Although the work schedule - exactly as in `real' science - was quite hard, especially during the following data reduction and interpretative phase, all teams managed extremely well and in high spirits. The young astronomers' observations were favoured by excellent atmospheric conditions. At the NTT, the seeing was better than 0.5 arcsecond during several hours, an exceptional value that allows very good images to be obtained. All observations represent solid and interesting science, and

  19. Shaping ESO2020+ Together: Feedback from the Community Poll

    Czech Academy of Sciences Publication Activity Database

    Primas, F.; Ivison, R.; Berger, J.-P.; Caselli, P.; De Gregorio-Monsalvo, I.; Alonso Herrero, A.; Knudsen, K.K.; Leibundgut, B.; Moitinho, A.; Saviane, I.; Spyromilio, J.; Testi, L.; Vennes, Stephane

    2015-01-01

    Roč. 161, September (2015), s. 6-14 ISSN 0722-6691 Institutional support: RVO:67985815 Keywords : ESO * observing programmes * data archiving Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics

  20. From Handicraft to Industry: Supporting Surveys at ESO Telescopes

    Science.gov (United States)

    Chavan, A. M.; Comerón, F.; Peron, M.; Canavan, T.; Dorigo, D.; Nunes, P.

    2006-07-01

    The ESO observatory at Cerro Paranal will host two new telescopes dedicated to survey-type observations. While surveys have already been conducted with existing ESO facilities, the features of the new ESO Survey Telescopes are a challenge for the Observatory's users and operations teams as well, in terms of number of observations and data rate. A set of software tools are being developed to help astronomers define the observation strategy and sequencing, while observatory staff are given assistance in managing and executing projects including hundreds or thousands of observations, and producing highly homogeneous datasets. This paper presents the extensions to ESO's 'Phase II' tools that will be implemented in the context of survey support, and discusses some of the challenges faced by software developers when problem sizes scale up one or more orders of magnitude.

  1. Autoimmune thyroid disease elicited by NY-ESO-1 vaccination.

    Science.gov (United States)

    Vita, Roberto; Guarneri, Fabrizio; Agah, Ravin; Benvenga, Salvatore

    2014-02-01

    Immunotherapies and targeted therapies are frequently associated with thyroid dysfunction, which is in contrast with the rare thyroid abnormalities induced by cytotoxic agents. Immunotherapy with NY-ESO-1, a tumor-associated antigen expressed by a number of malignancies, was reported to trigger hyperthyroidism or hypothyroidism in two HLA-A2 patients with ovarian cancer. We describe now a case of Graves' disease triggered by NY-ESO-1 in a HLA-A2-negative woman. A 32-year-old woman with a synovial sarcoma received radiotherapy, chemotherapy, and finally NY-ESO-1 vaccine. The patient was found to have HLA A11/A33(19), B13/B56(22), Cw3/-. One month after the beginning of immunotherapy, thyroid dysfunction was clinically suspected and Graves' disease was biochemically confirmed. Fearful of the antithyroid drugs' side effects, the patient was treated with a beta-blocker (propranolol, 80-20 mg/day). As hyperthyroidism progressively worsened, the patient underwent total thyroidectomy. We hypothesized that NY-ESO-1 shared partial homology with thyroid autoantigens (the so-called molecular mimicry mechanism) and that at least one pair of homologous sequences contained amino acid sequence binding motifs to a restricted number of HLA molecules. We used BLAST software to search amino acid sequence homologies between NY-ESO-1 and thyroid autoantigens (thyrotropin receptor [TSH-R], thyroperoxidase, and thyroglobulin), and the HLA ligand/motif database to look for HLA/T-cell receptor binding motifs in the regions of NY-ESO-1 and thyroid autoantigens that were homologous. We found 15 epitopic regions of NY-ESO-1 homologous to 15 regions of thyroid autoantigens, some of which epitopic: 5 of TSH-R, 8 of thyroglobulin, and 2 of thyroperoxidase. These homologous sequences contain binding motifs belonging to several HLA class I antigens, including HLA A2 and the patient's A11 and A33. Genetically predisposed patients who receive NY-ESO-1 vaccination are at risk to develop thyroid

  2. ESO Council Resolves to Continue VLT Project at Paranal

    Science.gov (United States)

    1995-06-01

    The ESO Council has just met at the ESO Headquarters in Garching during two days of intensive consultations. Among the important items on the agenda were the recent developments around the Very Large Telescope Project at Paranal, and also the possible membership of other countries. RELATIONS WITH CHILE The ESO Council appreciated the continued efforts by the Government of the Republic of Chile to bring to a successful conclusion the various pending matters concerning the relations between the Republic of Chile and this Organisation. It noted the solution of serious problems which have interfered with ESO's operations in its host country, and now awaits formal confirmation from the Chilean Government that also the legal proceedings around the land dispute around the Paranal site are cleared. Council expects that the ratification of the "Intermediate, Supplementary and Amending Agreement" will occur without undue delay and it reiterates its intention to approve this Agreement at the same time as the Chilean Parliament. Meanwhile, Council expressed its pleasure to welcome those Chilean scientists now nominated by the Chilean Government as observers to the ESO committees. Council instructed the ESO Management to continue work at Paranal. Although the delegates were increasingly optimistic that the VLT project can ultimately remain at Paranal, they nevertheless also requested the Management to pursue, until further notice, the ongoing comparative study of first-class astronomical sites in the world. In connection with the pending issues in the relationship with Chile, Council remained preoccupied about the recognition of ESO's immunities by the Chilean judiciary and it requested from the Management that the assessment of the financial and other implications of the recent events which have interfered with the VLT construction work at Paranal be completed. Finally, in order to provide additional avenues for frequent contacts, the ESO Council invited the Chilean Government

  3. ESO celebrates 50 years of “Euro-astronomy”

    CERN Multimedia

    Anaïs Schaeffer

    2012-01-01

    Today the European Southern Observatory is celebrating its 50th anniversary. This article retraces the story of one of Europe's greatest laboratories, which came into being with CERN's support and assistance.   La Silla, June 1968. The three telescopes in the background are (from left to right): the Grand Prism Objectif (GPO, first light in 1968), the ESO 1 metre telescope (1966), and the ESO 1.5 metre telescope (1968). They have been decommissioned. The white dome closest to the viewer is the ESO 1 metre Schmidt telescope, which began work in 1971. Image: ESO/E. Maurice. In the spring of 1953, in Leiden in the Netherlands, a group of astronomers including Walter Baade, Jan Oort, Adriaan Blaauw, Otto Heckmann and Jan Bannier (who was then President of CERN's Provisional Council) for the first time discussed the possibility of founding a European space observatory. The objective was to build it in the southern hemisphere where there were very few observatories with pow...

  4. Deep Sky Diving with the ESO New Technology Telescope

    Science.gov (United States)

    1998-01-01

    Preparations for future cosmological observations with the VLT Within a few months, the first 8.2-meter Unit Telescope of the ESO Very Large Telescope (VLT) array will open its eye towards the sky above the Atacama desert. As documented by recent Press Photos from ESO, the construction work at the Paranal VLT Observatory is proceeding rapidly. Virtually all of the telescope components, including the giant Zerodur mirror (cf. ESO PR Photos 35a-l/97 ), are now on the mountain. While the integration of the telescope and its many optical, mechanical and electronic components continues, astronomers in the ESO member countries and at ESO are now busy defining the observing programmes that will be carried out with the new telescope, soon after it enters into operation. In this context, new and exciting observations have recently been obtained with the 3.5-m New Technology Telescope at the ESO La Silla Observatory, 600 km to the south of Paranal. How to record the faintest and most remote astronomical objects With its very large mirror surface (and correspondingly great light collecting power), as well as an unsurpassed optical quality, the VLT will be able to look exceedingly far out into the Universe, well beyond current horizons. The best technique to record the faintest possible light and thus the most remote celestial objects, is to combine large numbers of exposures of the same field with slightly different telescope pointing. This increases the total number of photons recorded and by imaging the stars and galaxies on different areas (pixels) of the detector, the signal-to-noise ratio and hence the visibility of the faintest objects is improved. The famous Hubble Deep Field Images were obtained in this way by combining over 300 single exposures and they show myriads of faint galaxies in the distant realms of the Universe. The NTT as test bench for the VLT ESO is in the fortunate situation of possessing a `prototype' model of the Very Large Telescope, the 3.5-m New

  5. ESO Council Decides to Continue VLT Project at Paranal

    Science.gov (United States)

    1994-08-01

    The Council [1] of the European Southern Observatory has met in extraordinary session at the ESO Headquarters in Garching near Munich on August 8 and 9, 1994. The main agenda items were concerned with the recent developments around ESO's relations with the host state, the Republic of Chile, as well as the status of the organisation's main project, the 16-metre equivalent Very Large Telescope (VLT) which will become the world's largest optical telescope. Council had decided to hold this special meeting [2] because of various uncertainties that have arisen in connection with the implementation of the VLT Project at Cerro Paranal, approx. 130 kilometres south of Antofagasta, capital of the II Region in Chile. Following continued consultations at different levels within the ESO member states and after careful consideration of all aspects of the current situation - including various supportive actions by the Chilean Government as well as the incessive attacks against this international organisation from certain sides reported in the media in that country - Council took the important decision to continue the construction of the VLT Observatory at Paranal, while at the same time requesting the ESO Management to pursue the ongoing studies of alternative solutions. THE COUNCIL DECISIONS In particular, the ESO Council took note of recent positive developments which have occurred since the May 1994 round of discussions with the Chilean authorities in Santiago. The confirmation of ESO's immunities as an International Organization in Chile, contained in a number of important statements and documents, is considered a significant step by the Chilean Government to insure to ESO the unhindered erection and later operation of the VLT on Paranal. Under these circumstances and in order to maintain progress on the VLT project, the ESO Council authorized the ESO Management to continue the on-site work at Paranal. Council also took note of the desire expressed by the Chilean Government

  6. Faint Galaxies: Merging Model of E/SO Galaxies

    Science.gov (United States)

    Xu, Wen; Yu, Yunqiang

    1995-04-01

    We study a model of mergers affecting only the progenitors of present E/SO. We adopt the standard scenarios of star formation as prescribed by Guiderdoni & Rocca-Volmerange. The merging process is parametrized under the assumptions of(1) self-similarity of the Schechter MF and(2) mass conservation. Nine models are discussed. The predictions are compared with counts ofB J ,U +,F +,N + bands. E/SO mergers account for the excess of the faintest blue galaxies without causing excess in redder bands. However, as we no longer have enough mergers at brighter magnitudes, a plain E/SO merging model fits less tightly for the redshift and the colour distributions. Detection effect, a steeper slope of LF may be ways to improve. Our models predict acceptable merger frequencies atz = 0.5 although some models predict more interacting galaxies than observation atz = 0.

  7. NGC 3293 revisited by the Gaia-ESO Survey

    Science.gov (United States)

    Semaan, Thierry; Morel, Thierry; Gosset, Eric; Zorec, Juan; Frémat, Yves; Blomme, Ronny; Lobel, Alex

    2015-01-01

    In the framework of the Gaia-ESO survey we have determined the fundamental parameters of a large number of B-type stars in the Galactic, young open cluster NGC 3293. The determination of the stellar parameters is based on medium-resolution spectra obtained with FLAMES/GIRAFFE at ESO-VLT. As a second step, we adopted the accurate parameters to determine the chemical abundances of these hot stars. We present a comparison of our results with those obtained by the 'VLT-FLAMES survey of massive stars' (Evans et al. 2005). Our study increases the number of objects analysed and provides an extended view of this cluster.

  8. The ESO Educational Office Reaches Out towards Europe's Teachers

    Science.gov (United States)

    2001-12-01

    ESA/ESO Astronomy Exercises Provide a Taste of Real Astronomy [1] Summary The European Southern Observatory (ESO) has been involved in many Europe-wide educational projects during the past years, in particular within European Science Weeks sponsored by the European Commission (EC). In order to further enhance the significant educational potential inherent in the numerous scientific endeavours now carried out by Europe's astronomers with ESO front-line telescope facilities, it has been decided to set up an Educational Office within the ESO EPR Department. It will from now on work closely with astronomy-oriented teachers, in particular at the high-school level , providing support, inspiration and new materials. Much of this interaction will happen via the European Association for Astronomy Education (EAAE) In this context, and in collaboration with the European Space Agency (ESA) , the first instalments of the "ESA/ESO Astronomy Exercise Series" have just been published, on the web ( http://www.astroex.org ) and in print (6 booklets totalling 100 pages; provided free-of-charge to teachers on request). They allow 16-19 year old students to gain exciting hands-on experience in astronomy, making realistic calculations with data obtained from observations by some of the world's best telescopes, the NASA/ESA Hubble Space Telescope (HST) and ESO's Very Large Telescope (VLT) . PR Photo 36/01 : The "ESA/ESO Astronomy Exercise Series" . Educational projects at ESO The European Southern Observatory (ESO) , through its Education and Public Relations Department (EPR) , has long been involved in educational activities, in particular by means of Europe-wide projects during successive European Science Weeks , with support from the European Commission (EC) . A most visible outcome has been the creation of the trailblazing European Association for Astronomy Education (EAAE) - this was first discussed at an international meeting at the ESO Headquarters in November 1994 with the

  9. The ESO Survey of Non-Publishing Programmes

    Science.gov (United States)

    Patat, F.; Boffin, H. M. J.; Bordelon, D.; Grothkopf, U.; Meakins, S.; Mieske, S.; Rejkuba, M.

    2017-12-01

    One of the classic ways to measure the success of a scientific facility is the publication return, which is defined as the refereed papers produced per unit of allocated resources (for example, telescope time or proposals). The recent studies by Sterzik et al. (2015, 2016) have shown that 30–50 % of the programmes allocated time at ESO do not produce a refereed publication. While this may be inherent to the scientific process, this finding prompted further investigation. For this purpose, ESO conducted a Survey of Non-Publishing Programmes (SNPP) within the activities of the Time Allocation Working Group, similar to the monitoring campaign that was recently implemented at ALMA (Stoehr et al., 2016). The SNPP targeted 1278 programmes scheduled between ESO Periods 78 and 90 (October 2006 to March 2013) that had not published a refereed paper as of April 2016. The poll was launched on 6 May 2016, remained open for four weeks, and returned 965 valid responses. This article summarises and discusses the results of this survey, the first of its kind at ESO.

  10. ESDIS Standards Office (ESO): Requirements, Standards and Practices

    Science.gov (United States)

    Mitchell, Andrew E.; Mcinerney, Mark Allen; Enloe, Yonsok K.; Conover, Helen T.; Doyle, Allan

    2016-01-01

    The ESDIS Standards Office assists the ESDIS Project in formulating standards policy for NASA Earth Science Data Systems (ESDS), coordinates standards activities within ESDIS, and provides technical expertise and assistance with standards related tasks within the NASA Earth Science Data System Working Groups (ESDSWG). This poster summarizes information found on the earthdata.nasa.gov site that describes the ESO.

  11. PHOENIX: the production line for science data products at ESO

    OpenAIRE

    Hanuschik, Reinhard

    2015-01-01

    In the past two years ESO has installed a production line for level 2 science data products. Focussing on spectroscopic observations, these in-house generated data products are complementary to the externally provided data products from the imaging surveys. The production line combines mass production (more than one million spectra have been generated so far), previews, and quality control.

  12. ESO & NOT photometric monitoring of the Cloverleaf quasar

    NARCIS (Netherlands)

    Ostensen, R; Remy, M; Lindblad, PO; Refsdal, S; Stabell, R; Surdej, J; Barthel, PD; Emanuelsen, PI; Festin, L; Gosset, E; Hainaut, O; Hakala, P; Hjelm, M; Hjorth, J; Hutsemekers, D; Jablonski, M; Kaas, AA; Kristen, H; Larsson, S; Magain, P; Pettersson, B; Pospieszalska-Surdej, A; Smette, A; Teuber, J; Thomsen, B; Van Drom, E

    1997-01-01

    The Cloverleaf quasar, H1413+117, has been photometrically monitored at ESO (La Silla, Chile) and with the NOT (La Palma, Spain) during the period 1987-1994. All good quality CCD frames have been successfully analysed using two independent methods (i.e. an automatic image decomposition technique and

  13. NY-ESO-1 cancer testis antigen demonstrates high immunogenicity in triple negative breast cancer.

    Science.gov (United States)

    Ademuyiwa, Foluso O; Bshara, Wiam; Attwood, Kristopher; Morrison, Carl; Edge, Stephen B; Karpf, Adam R; James, Smith A; Ambrosone, Christine B; O'Connor, Tracey L; Levine, Ellis G; Miliotto, Anthony; Ritter, Erika; Ritter, Gerd; Gnjatic, Sacha; Odunsi, Kunle

    2012-01-01

    NY-ESO-1 cancer testis (CT) antigen is an attractive candidate for immunotherapy as a result of its high immunogenicity. The aim of this study was to explore the potential for NY-ESO-1 antigen directed immunotherapy in triple negative breast cancer (TNBC) by determining the frequency of expression by immunohistochemistry (IHC) and the degree of inherent immunogenicity to NY-ESO-1. 168 TNBC and 47 ER+/HER2- primary breast cancer specimens were used to determine NY-ESO-1 frequency by IHC. As previous studies have shown that patients with a robust innate humoral immune response to CT antigens are more likely to develop CD8 T-cell responses to NY-ESO-1 peptides, we evaluated the degree to which patients with NY-ESO-1 expression had inherent immunogenicity by measuring antibodies. The relationship between NY-ESO-1 expression and CD8+ T lymphocytes was also examined. The frequency of NY-ESO-1 expression in the TNBC cohort was 16% versus 2% in ER+/HER2- patients. A higher NY-ESO-1 score was associated with a younger age at diagnosis in the TNBC patients with NY-ESO-1 expression (p = 0.026). No differences in OS (p = 0.278) or PFS (p = 0.238) by NY-ESO-1 expression status were detected. Antibody responses to NY-ESO-1 were found in 73% of TNBC patients whose tumors were NY-ESO-1 positive. NY-ESO-1 positive patients had higher CD8 counts than negative patients (p = 0.018). NY-ESO-1 is expressed in a substantial subset of TNBC patients and leads to a high humoral immune response in a large proportion of these individuals. Given these observations, patients with TNBC may benefit from targeted therapies directed against NY-ESO-1.

  14. Photographic photometry of 16000 galaxies on ESO blue and red survey plates

    NARCIS (Netherlands)

    Lauberts, A.; Valentijn, E. A.

    1985-01-01

    An ongoing photometric survey of 16,000 galaxies on ESO red and blue plates is described. The galaxies of the ESO/Uppsala Catalog (Lauberts, 1982) are being PDS scanned, and the results are combined with existing photometry and supplementary observational data obtained at the 1-m ESO telescope to

  15. ESO Council Visits First VLT Unit Telescope Structure in Milan

    Science.gov (United States)

    1995-12-01

    As the ESO Very Large Telescope (VLT) rapidly takes on shape, Europe has just come one step closer to the realisation of its 556 million DEM astronomical showcase project. Last week, the ESO Council held its semi-annual meeting in Milan (Italy) [1]. During a break in the long agenda list, Council members had the opportunity to visit the Ansaldo factory in the outskirts of this city and to see for the first time the assembled mechanical structure of one of the four 8.2-metre VLT Unit telescopes. This Press Release is accompanied by a photo that shows the ESO Council delegates in front of the giant telescope. After a long climb up the steep staircase to the large Nasmyth platform at the side of the telescope where the astronomical instruments will later be placed, Dr. Peter Creola (Switzerland) , President of the ESO Council and a mechanics expert, grabbed the handrail and surveyed the structure with a professional eye: `I knew it was going to be big, but not that enormous!', he said. Other delegates experienced similar feelings, especially when they watched the 430 tonnes of steel in the 24-metre tall and squat structure turn smoothly and silently around the vertical axis. The Chairman of the ESO Scientific Technical Committee (STC), Dr. Johannes Andersen (Denmark) , summarized his first, close encounter with the VLT by `This is great fun!' and several of his colleague astronomers were soon seen in various corners of the vast structure, engaged in elated discussions about the first scientific investigations to be done with the VLT in two years' time. The VLT Main Structures The visit by Council took place at the invitation of Ansaldo Energia S.p.A. (Genova), EIE-European Industrial Engineering S.r.I. (Venice) and SOIMI-Societa Impianti Industriale S.p.A. (Milan), the three Italian enterprises responsible for the construction of the main structures of the VLT 8.2-metre Unit telescopes. Short speeches were given on this occasion by Drs. Ferruccio Bressani (Ansaldo

  16. Participant Perspectives on the ESO Astronomy Camp Programme

    Science.gov (United States)

    Olivotto, C.; Cenadelli, D.; Gamal, M.; Grossmann, D.; Teller, L. A. I.; Marta, A. S.; Matoni, C. L.; Taillard, A.

    2015-09-01

    This article describes the experience of attending the European Southern Observatory (ESO) Astronomy Camp from the perspective of its participants - students aged between 16 and 18 years old from around the world. The students shared a week together during the winter of 2014 in the Alpine village of Saint-Barthelemy, Italy. The camp was organised by ESO in collaboration with Sterrenlab and the Astronomical Observatory of the Autonomous Region of the Aosta Valley and offered a rich programme of astronomy and leisure activities. This article focuses on the concept of astronomy camps, and their role as a unique tool to complement formal classroom education, rather than on the astronomy activities and the scientific programme. Thus, it is not an academic review of the implemented methodologies, but rather a reflection on the overall experience. The article was brought together from collaborative accounts by some of the participants who were asked to reflect on the experience. The participants who contributed to this article represent the diversity of the ESO Astronomy Camp's alumni community.

  17. Vaccination with NY-ESO-1 overlapping peptides mixed with Picibanil OK-432 and montanide ISA-51 in patients with cancers expressing the NY-ESO-1 antigen.

    Science.gov (United States)

    Wada, Hisashi; Isobe, Midori; Kakimi, Kazuhiro; Mizote, Yu; Eikawa, Shingo; Sato, Eiichi; Takigawa, Nagio; Kiura, Katsuyuki; Tsuji, Kazuhide; Iwatsuki, Keiji; Yamasaki, Makoto; Miyata, Hiroshi; Matsushita, Hirokazu; Udono, Heiichiro; Seto, Yasuyuki; Yamada, Kazuhiro; Nishikawa, Hiroyoshi; Pan, Linda; Venhaus, Ralph; Oka, Mikio; Doki, Yuichiro; Nakayama, Eiichi

    2014-01-01

    We conducted a clinical trial of an NY-ESO-1 cancer vaccine using 4 synthetic overlapping long peptides (OLP; peptides #1, 79-108; #2, 100-129; #3, 121-150; and #4, 142-173) that include a highly immunogenic region of the NY-ESO-1 molecule. Nine patients were immunized with 0.25 mg each of three 30-mer and a 32-mer long NY-ESO-1 OLP mixed with 0.2 KE Picibanil OK-432 and 1.25 mL Montanide ISA-51. The primary endpoints of this study were safety and NY-ESO-1 immune responses. Five to 18 injections of the NY-ESO-1 OLP vaccine were well tolerated. Vaccine-related adverse events observed were fever and injection site reaction (grade 1 and 2). Two patients showed stable disease after vaccination. An NY-ESO-1-specific humoral immune response was observed in all patients and an antibody against peptide #3 (121-150) was detected firstly and strongly after vaccination. NY-ESO-1 CD4 and CD8 T-cell responses were elicited in these patients and their epitopes were identified. Using a multifunctional cytokine assay, the number of single or double cytokine-producing cells was increased in NY-ESO-1-specific CD4 and CD8 T cells after vaccination. Multiple cytokine-producing cells were observed in PD-1 (-) and PD-1 (+) CD4 T cells. In conclusion, our study indicated that the NY-ESO-1 OLP vaccine mixed with Picibanil OK-432 and Montanide ISA-51 was well tolerated and elicited NY-ESO-1-specific humoral and CD4 and CD8 T-cell responses in immunized patients.

  18. NY-ESO-1 antibody as a novel tumour marker of gastric cancer.

    Science.gov (United States)

    Fujiwara, S; Wada, H; Kawada, J; Kawabata, R; Takahashi, T; Fujita, J; Hirao, T; Shibata, K; Makari, Y; Iijima, S; Nishikawa, H; Jungbluth, A A; Nakamura, Y; Kurokawa, Y; Yamasaki, M; Miyata, H; Nakajima, K; Takiguchi, S; Nakayama, E; Mori, M; Doki, Y

    2013-03-19

    NY-ESO-1 antibodies are specifically observed in patients with NY-ESO-1-expressing tumours. We analysed whether the NY-ESO-1 humoral immune response is a useful tumour marker of gastric cancer. Sera from 363 gastric cancer patients were screened by enzyme-linked immunosorbent assay (ELISA) to detect NY-ESO-1 antibodies. Serial serum samples were obtained from 25 NY-ESO-1 antibody-positive patients, including 16 patients with curative resection and 9 patients who received chemotherapy alone. NY-ESO-1 antibodies were detected in 3.4% of stage I, 4.4% of stage II, 25.3% of stage III, and 20.0% of stage IV patients. The frequency of antibody positivity increased with disease progression. When the NY-ESO-1 antibody was used in combination with carcinoembryonic antigen and CA19-9 to detect gastric cancer, information gains of 11.2% in stages III and IV, and 5.8% in all patients were observed. The NY-ESO-1 immune response levels of the patients without recurrence fell below the cutoff level after surgery. Two of the patients with recurrence displayed incomplete decreases. The nine patients who received chemotherapy alone continued to display NY-ESO-1 immune responses. When combined with conventional tumour markers, the NY-ESO-1 humoral immune response could be a useful tumour marker for detecting advanced gastric cancer and inferring the post-treatment tumour load in seropositive patients.

  19. NY-ESO-1 expression and immunogenicity in prostate cancer patients.

    Science.gov (United States)

    Gati, Asma; Lajmi, Nesrine; Derouiche, Amine; Marrakchi, Raja; Chebil, Mohamed; Benammar-Elgaaied, Amel

    2011-10-01

    Prostate cancer is the second leading cause of men cancer-related death. Cancer immunotherapy has been investigated as a treatment which might be instituted at the point of detection of androgen-independent metastatic disease. to investigate the expression and humoral response against NYESO-1 in patients with prostate cancer (PC) and to analyze the relationship between expression of NY-ESO-1 and clinicopathological features. NY-ESO-1 mRNA in surgically resected PC and benign prostatic hyperplasia (BPH) were examined by reverse transcriptionpolymerase chain reaction. The antibody response to NY-ESO-1 was examined by enzyme-linked Elisa assay using recombinant NYESO-1 protein. NY-ESO-1 mRNA was detected in 9 of 23 (39%) PC patients. Antibodies against NY-ESO-1 protein were detected in 12 of 23 (52%) sera of PC patients and in 5 of 9 (55%) of NY-ESO-1 expressing tumors. However, no mRNA copy or NY-ESO-1 antibodies were detected in all BPH patients tested. The present study has demonstrated the expression of NY-ESO-1mRNA in prostate Cancer patients and NY-ESO-1 antibody production. Our data suggest that NY-ESO-1 could be used as a tumor marker and constitute a good candidate for vaccine-based immunotherapy for hormonal resistant prostate cancer patients.

  20. NY-ESO-1 expression predicts an aggressive phenotype of ovarian cancer.

    Science.gov (United States)

    Szender, J Brian; Papanicolau-Sengos, Antonios; Eng, Kevin H; Miliotto, Anthony J; Lugade, Amit A; Gnjatic, Sacha; Matsuzaki, Junko; Morrison, Carl D; Odunsi, Kunle

    2017-06-01

    NY-ESO-1 is a cancer testis antigen and a promising target for immunotherapy. The purpose of this study was to determine the expression frequency, immunogenicity, and clinical impact of NY-ESO-1 in ovarian cancer. Immunohistochemistry (IHC), reverse-transcription polymerase chain reaction (RT-PCR), and quantitative-PCR (qRT-PCR) were utilized in an ovarian cancer (including Fallopian tube and primary peritoneal cancers) patient cohort; humoral responses against NY-ESO-1 were determined by ELISA. Clinicopathologic outcomes including progression-free (PFS) and overall (OS) survival were evaluated based on NY-ESO-1 expression. Cohen's kappa (κ) tested agreement between expression tests. NY-ESO-1 expression was detected by any method in 40.7% of 1002 patients' tumors (NY-ESO-1+) and baseline humoral response was identified in 19.0% of 689 tested patients. NY-ESO-1+ patients were older (pESO-1+ patients. A subset analysis of NY-ESO-1+ patients that received immunotherapy demonstrated improved OS by >2years (52.6 vs. 27.2months, pESO-1 expression and an aggressive cancer phenotype. The relatively high expression frequency of NY-ESO-1 in ovarian cancer patients coupled with the poor clinical outcomes in NY-ESO-1+ patients reveals an underappreciated need for targeted therapy against this antigen. In support, our study reveals that NY-ESO-1+ patients enrolled on immunotherapy trials targeting the antigen exhibited an improvement in OS. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. Plans for a fast image recording system at ESO

    Science.gov (United States)

    Grosboel, P. J.

    1984-01-01

    A 256 diode-array will be installed as detector on the ESO OPTRONICS S-3000 measuring machine in order to increase the acquisition rate. A high intensity LED will be used as light source in a pulse mode. The data will be stored on a random access mass storage device as density values for later education. The scanning time for a 30 cm x 30 cm plate with a step size of 10 micron will be less than 10 hours while the dynamic range of the data is expected to be 2.5 density units with an offset of at least 1 unit.

  2. First Giant Mirror for the ESO VLT Ready at REOSC

    Science.gov (United States)

    1995-11-01

    The REOSC Contract In 1989, the European Southern Observatory (ESO), the European Organisation for Astronomy, awarded to REOSC, a subsidiary of the SFIM Group and located in Saint Pierre du Perray (France), a comprehensive contract for the polishing of four 8.2-metre diameter mirrors for the unit telescopes of the ESO Very Large Telescope (VLT) project. These mirrors are the largest ever manufactured and polished. This contract comprises not only the polishing and high-precision optical testing of each giant mirror, but also the safe condition of transportation of the blanks which were manufactured by Schott Glaswerke in Mainz (Germany). In order to fulfill the contract, REOSC conceived, built and equipped a novel, high-tech workshop which would allow to polish and test the mirrors, each of which has a surface area of more than 50 square metres. First 8.2-Metre Mirror is Ready and within Specifications The REOSC polishing facility for giant mirrors was built in Saint Pierre du Perray, just south of Paris. It is equipped with two machines: one for grinding and the other for polishing the mirrors, and both with 150-actuator systems that support the thin and flexible mirrors. All equipment is computer controlled. State-of-the-art interferometers probe the accuracy of the mirror surface as the polishing proceeds; they are installed at the top level of the facility in a 30-metre high tower, at the centre of the mirror's radius of curvature. The success of the work at REOSC is now evident by the fact that careful measurements of the first mirror earlier this month have shown that the final optical surface is correct to within 0.00005 millimetres. For illustration, this corresponds to an accuracy of only 1 millimetre deviation over a surface with a diameter of 165 kilometres (equivalent to the entire Paris area)! ESO Receives the First VLT Mirror After having been carefully placed in a special transport box designed by REOSC, the first mirror blank, weighing 23.5 tons and

  3. Cuadernos de Geografia para la ESO de la Editorial ECIR

    Directory of Open Access Journals (Sweden)

    Eugenio García Almiñana

    2005-12-01

    Full Text Available INTRODUCCIÓN:Uno de los problemas actuales en la enseñanza de la Geografia es la atención al alurnnado que tiene problemas especiales en la Enseñanza Secundaria Obligatoria. Esos problemas pueden venir bien de una falta de motivación, bien porque hay interés pero no capacidades suficientes, podemos encontrar casos de verdadero absentismo escolar, es decir, alumnos que se niegan sistemáticamente a manejar siquiera los materiales que le proporcionan sus profesores y otros que tieneninterés pero son inmigrantes extranjeros con difícultades de aprendizaje y de lengua.

  4. Report on the ''ESO Python Boot Camp — Pilot Version''

    Science.gov (United States)

    Dias, B.; Milli, J.

    2017-03-01

    The Python programming language is becoming very popular within the astronomical community. Python is a high-level language with multiple applications including database management, handling FITS images and tables, statistical analysis, and more advanced topics. Python is a very powerful tool both for astronomical publications and for observatory operations. Since the best way to learn a new programming language is through practice, we therefore organised a two-day hands-on workshop to share expertise among ESO colleagues. We report here the outcome and feedback from this pilot event.

  5. CERN, ESA and ESO Launch "Physics On Stage"

    Science.gov (United States)

    2000-03-01

    Physics is everywhere . The laws of physics govern the Universe, the Sun, the Earth and even our own lives. In today's rapidly developing society, we are becoming increasingly dependent on high technology - computers, transport, and communication are just some of the key areas that are the result of discoveries by scientists working in physics. But how much do the citizens of Europe really know about physics? Here is a unique opportunity to learn more about this elusive subject! [Go to Physics On Stage Website] Beginning in February 2000, three major European research organisations are organising a unique Europe-wide programme to raise the public awareness of physics and related sciences. "Physics on Stage" is launched by the European Laboratory for Particle Physics (CERN) , the European Space Agency (ESA) and the European Southern Observatory (ESO) , with support from the European Union. Other partners are the European Physical Society (EPS) and the European Association for Astronomy Education (EAAE). This exciting programme is part of the European Week for Science and Technology and will culminate in a Science Festival during November 6-11, 2000, on the CERN premises at the French-Swiss border near Geneva. Why "Physics on Stage"? The primary goal of "Physics on Stage" is to counteract the current decline in interest and knowledge about physics among Europe's citizens by means of a series of highly visible promotional activities. It will bring together leading scientists and educators, government bodies and the media, to confront the diminishing attraction of physics to young people and to develop strategies to reverse this trend. The objective in the short term is to infuse excitement and to provide new educational materials. In the longer term, "Physics on Stage" will generate new developments by enabling experts throughout Europe to meet, exchange and innovate. "Physics on Stage" in 22 European Countries "Physics on Stage" has been initiated in 22 European

  6. NY-ESO-1 expression in synovial sarcoma and other mesenchymal tumors: significance for NY-ESO-1-based targeted therapy and differential diagnosis.

    Science.gov (United States)

    Lai, Jin-Ping; Robbins, Paul F; Raffeld, Mark; Aung, Phyu Phyu; Tsokos, Maria; Rosenberg, Steven A; Miettinen, Markku M; Lee, Chyi-Chia Richard

    2012-06-01

    A promising targeted therapy against NY-ESO-1 (CTAG 1B) using genetically modified T-cells in synovial sarcomas was recently demonstrated in a clinical trial at the NCI. To investigate the role of NY-ESO-1 immunohistochemistry in patient selection and gain better insight into the incidence of NY-ESO-1 expression in synovial sarcomas and other mesenchymal tumors, we evaluated NY-ESO-1 expression by immunohistochemistry in 417 tumors. This collection of samples included: 50 SS18/SSX1/2 fusion positive synovial sarcomas, 155 gastrointestinal stromal tumors (GIST), 135 other spindle cell sarcomas as well as 77 other sarcomas (chondrosarcoma, osteosarcoma, dedifferentiated liposarcoma, alveolar soft part sarcoma, rhabdomyosarcoma, angiosarcoma, malignant mesothelioma, and Ewing's sarcoma). We report that 76% of synovial sarcomas expressed NY-ESO-1 in a strong and diffuse pattern (2-3+, >50-70% of tumor cells). In contrast, only rare cases of other spindle cell mesenchymal tumor expressed NY-ESO-1 (GIST (2/155), malignant peripheral nerve sheath tumors (1/34), and dermatofibrosarcoma protuberans (2/20)). Individual cases of other sarcomas (angiosarcoma, malignant mesothelioma, chondrosarcoma, osteosarcoma, dedifferentiated liposarcoma, alveolar soft part sarcoma, and Ewing's sarcoma) were positive for NY-ESO-1. However, no positive cases were identified amongst our cohort of leiomyosarcomas (0/24), hemangiopericytoma/solitary fibrous tumors (0/40), and cellular schwannomas (0/17). In summary, we find that NY-ESO-1 is strongly and diffusely expressed in a majority of synovial sarcomas, but only rarely in other mesenchymal lesions. Beyond its role in patient selection for targeted therapy, immunohistochemistry for NY-ESO-1 may be diagnostically useful for the distinction of synovial sarcoma from other spindle cell neoplasms.

  7. NY-ESO-1 protein glycosylated by yeast induces enhanced immune responses.

    Science.gov (United States)

    Wadle, Andreas; Mischo, Axel; Strahl, Sabine; Nishikawa, Hiroyoshi; Held, Gerhard; Neumann, Frank; Wullner, Beate; Fischer, Eliane; Kleber, Sascha; Karbach, Julia; Jager, Elke; Shiku, Hiroshi; Odunsi, Kunle; Shrikant, Protul A; Knuth, Alexander; Cerundolo, Vincenzo; Renner, Christoph

    2010-11-01

    Vaccine strategies that target dendritic cells to elicit potent cellular immunity are the subject of intense research. Here we report that the genetically engineered yeast Saccharomyces cerevisiae, expressing the full-length tumour-associated antigen NY-ESO-1, is a versatile host for protein production. Exposing dendritic cells (DCs) to soluble NY-ESO-1 protein linked to the yeast a-agglutinin 2 protein (Aga2p) protein resulted in protein uptake, processing and MHC class I cross-presentation of NY-ESO-1-derived peptides. The process of antigen uptake and cross-presentation was dependent on the glycosylation pattern of NY-ESO-1-Aga2p protein and the presence of accessible mannose receptors. In addition, NY-ESO-1-Aga2p protein uptake by dendritic cells resulted in recognition by HLA-DP4 NY-ESO-1-specific CD4(+) T cells, indicating MHC class II presentation. Finally, vaccination of mice with yeast-derived NY-ESO-1-Aga2p protein led to an enhanced humoral and cellular immune response, when compared to the bacterially expressed NY-ESO-1 protein. Together, these data demonstrate that yeast-derived full-length NY-ESO-1-Aga2p protein is processed and presented efficiently by MHC class I and II complexes and warrants clinical trials to determine the potential value of S. cerevisiae as a host for cancer vaccine development. Copyright © 2010 John Wiley & Sons, Ltd.

  8. Increased NY-ESO-1 expression and reduced infiltrating CD3+ T cells in cutaneous melanoma.

    Science.gov (United States)

    Giavina-Bianchi, Mara; Giavina-Bianchi, Pedro; Sotto, Mirian Nacagami; Muzikansky, Alona; Kalil, Jorge; Festa-Neto, Cyro; Duncan, Lyn M

    2015-01-01

    NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79), rarely in in situ melanoma (1/10) and not in benign nevi (0/20). Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. NY-ESO-1 expression was associated with increased primary tumor thickness (P = 0.007) and inversely correlated with superficial spreading melanoma (P ESO-1 expression was also associated with reduced numbers and density of CD3+ tumor infiltrating lymphocytes (P = 0.017). When NY-ESO-1 protein was expressed, CD3+ T cells were less diffusely infiltrating the tumor and were more often arranged in small clusters (P = 0.010) or as isolated cells (P = 0.002) than in large clusters of more than five lymphocytes. No correlation of NY-ESO-1 expression with gender, age, tumor site, ulceration, lymph node sentinel status, or survival was observed. NY-ESO-1 expression in melanoma was associated with tumor progression, including increased tumor thickness, and with reduced tumor infiltrating lymphocytes.

  9. Anti-NY-ESO-1 autoantibody may be a tumor marker for intrahepatic cholangiocarcinoma.

    Science.gov (United States)

    Zhang, Zhen; Li, Fan-Fan; Lu, Ming-Dian; Zhang, Shang-Xin; Li, Yong-Xiang

    2017-11-28

    Anti-NY-ESO-1 antibody is observed in a multitude of malignancies. This study was aimed to evaluate the expression of serum anti-NY-ESO-1 antibodies and its prognostic value in intrahepatic cholangiocarcinoma. A total of 103 patients with intrahepatic cholangiocarcinoma were enrolled in the study. Enzyme-linked immunosorbent assay (ELISA) was performed to detect the serum level of anti-NY-ESO-1 antibody. Western blotting was performed to assess the NY-ESO-1 expression in tumor and adjacent tissues. The serum NY-ESO-1 antibody was detected in 18.4% of patients with intrahepatic cholangiocarcinoma, a value that was significantly higher than that in patients with chronic Hepatitis B. Serum NY-ESO-1 antibody was positively correlated with tumor differentiation, lymphatic metastasis, cTNM stage and abdominal pain. Finally, there was a higher cumulative survival rate in patients with serum NY-ESO-1 positivity than in those with serum NY-ESO-1 negativity among the patients with stage III + IV. Our data uncovered that NY-ESO-1 antibody might be a helpful tumor marker and prognostic predictor in intrahepatic cholangiocarcinoma.

  10. Application of serum NY-ESO-1 antibody assay for early SCLC diagnosis.

    Science.gov (United States)

    Yang, Jihua; Jiao, Shunchang; Kang, Jingbo; Li, Rong; Zhang, Guanzhong

    2015-01-01

    NY-ESO-1 antibody is one of the cancer-related antibodies. The purpose of this study was to investigate the diagnostic role of the NY-ESO-1 humoral immune response in small cell lung cancer (SCLC). We recombined the recombinant protein of NY-ESO-1 antibody and NSE, analyzed them by Enzyme-linked immunosorbent assay, and then established the Receiver Operating Characteristic (ROC) curve to estimate the diagnostic value of NY-ESO-1 antibody, NSE and their combinations. According to detection, the positive rate of NY-ESO-1 humoral immune response (26.3%), NSE (43.8%) and their combinations (10.5%) were all lower than the negative rate which indicated that the NY-ESO-1 antibody might be down-regulated in SCLC. And the positive rate wasn't related to clinicopathologic characteristics. The ROC curve demonstrated that with a 37.17% sensitivity and a 91.7% specificity along with a AUC of 0.619 for NY-ESO-1ab as well as with a 48.3% sensitivity and a 90.87% specificity along with a AUC value of 0.773 for NSE, their diagnostic value were both high. Besides, the diagnostic value of their combinations was also good for a AUC of 0.83 and a 69.12% sensitivity and a 91.8% specificity. There were significant difference of diagnostic value among three types above (NY-ESO-1 vs. NSE, P ESO-1, P ESO-1ab, NSE and their combinations all were important diagnostic markers for SCLC. Moreover, the diagnostic value of their combinations was higher than any single of them. And NY-ESO-1 humoral immune to NSE might be a potential diagnostic indicator in SCLC.

  11. ESO Advanced Data Products for the Virtual Observatory

    Science.gov (United States)

    Retzlaff, J.; Delmotte, N.; Rite, C.; Rosati, P.; Slijkhuis, R.; Vandame, B.

    2006-07-01

    Advanced Data Products, that is, completely reduced, fully characterized science-ready data sets, play a crucial role for the success of the Virtual Observatory as a whole. We report on on-going work at ESO towards the creation and publication of Advanced Data Products in compliance with present VO standards on resource metadata. The new deep NIR multi-color mosaic of the GOODS/CDF-S region is used to showcase different aspects of the entire process: data reduction employing our MVM-based reduction pipeline, calibration and data characterization procedures, standardization of metadata content, and, finally, a prospect of the scientific potential illustrated by new results on deep galaxy number counts.

  12. Massive Stars in the Gaia-ESO Survey

    Science.gov (United States)

    Blomme, Ronny; Fremat, Yves; Lobel, Alex; Maíz Apellániz, Jesús; Morel, Thierry; Negueruela, Ignacio; Semaan, Thierry

    2013-06-01

    The Gaia-ESO Survey (GES) is an ambitious project to study the formation and evolution of the Milky Way and its stellar populations. It is led by Gerry Gilmore and Sofia Randich and includes about 350 Co-Investigators. During 300 nights (spread over 5 years) of order 10^5 Giraffe spectra and 10^4 UVES spectra will be taken. As part of the survey, about 13 clusters will be observed that were chosen specifically for their massive-star content. We report on the preliminary analysis of GES data from two such clusters: NGC 3293 and NGC 6705. We determine stellar parameters for the B-type stars in NGC 3293 and compare the A-type stars population between the two clusters. We also use a repeat observation to study binarity and use the radial velocity information to study cluster membership in NGC 3293. We also list our plans for future observations, which include the Carina nebula region.

  13. ESO 113-IG45 galaxy and/or quasar?

    CERN Document Server

    West, R M; Danks, A C

    1978-01-01

    Spectroscopy, UBV photometry and photography have been obtained of the extraordinary 13th magnitude object ESO 113-IG45 identified as a Seyfert galaxy by Fairall (1977); R.A.=01/sup h/ 21/sup m/.9; Decl .=-59 degrees 04' (1950). V/sub 0/=13630+or-50 km s/sup -1/; M/sub V /=-24/sup m/.0; largest diameter 75 kpc or more (with H/sub 0/=55 km s /sup -1/ Mpc/sup -1/). The nucleus is stellar-like and several times more luminous than the surrounding envelope which has a well-developed lane-structure. It is the intrinsically most luminous Seyfert nuclear yet known, and may be described as a 'quasar in the center of a (spiral) galaxy'. It is probably associated with the X-ray source 2A0120-591. (14 refs).

  14. VizieR Online Data Catalog: Surface Photometry of ESO-Uppsala Galaxies (Lauberts+ 1989)

    NARCIS (Netherlands)

    Lauberts, A.; Valentijn, E. A.

    2006-01-01

    The 15467 southern galaxies in this catalog were scanned from 407 blue and 407 red original ESO-Schmidt plates using a PDS microdensitometer. The galaxies were selected from the ESO-Uppsala Catalogue (VII/34). The original selection criterion was a minimum visual angular diameter of 1arcmin. The

  15. NY-ESO-1-specific immunological pressure and escape in a patient with metastatic melanoma.

    Science.gov (United States)

    von Boehmer, Lotta; Mattle, Muriel; Bode, Peter; Landshammer, Alexandro; Schäfer, Carolin; Nuber, Natko; Ritter, Gerd; Old, Lloyd; Moch, Holger; Schäfer, Niklaus; Jäger, Elke; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    During cancer progression, malignant cells may evade immunosurveillance. However, evidence for immunological escape in humans is scarce. We report here the clinical course of a melanoma patient whose initial tumor was positive for the antigens NY-ESO-1, MAGE-C1, and Melan-A. Upon immunization with a recombinant vaccinia/fowlpox NY-ESO-1 construct, the patient experienced a mixed clinical response and spreading of the NY-ESO-1 epitopes in the CD4+ T cell compartment. After NY-ESO-1 protein + CpG immunization, the patient's anti-NY-ESO-1 IgG response increased. Over the following years, progressing lesions were resected and found to be NY-ESO-1-negative while being positive for MAGE-C1, Melan-A, and MHC-I. The fatal, inoperable brain metastasis was analyzed after his death and also proved to be NY-ESO-1-negative, while being positive for MAGE-C1 and Melan-A, as well as MHC-I. We propose that cancer control and cancer escape in this patient were governed by NY-ESO-1-specific immunological pressure. Our findings provide evidence for the existence of immunoediting and immunoescape in this cancer patient.

  16. Expression and immunogenicity of NY-ESO-1 in colorectal cancer.

    Science.gov (United States)

    Li, Ya; Song, Ruifeng; Li, Xinqiang; Xu, Feng

    2017-06-01

    Serum assays of NY-ESO-1 antibodies provide a guide to discriminate between patients who suffer from different types of cancer. In the present study, the expression of NY-ESO-1 was detected with the aim to identify a novel tumor antigen in colorectal cancer (CRC). Sera were obtained from 89 healthy individuals and 236 patients with CRC with stage I, II, III and IV tumors. The NY-ESO-1 autoantibody levels were determined using an enzyme-linked immunosorbent assay. The mRNA and protein expression levels of NY-ESO-1 were detected using reverse transcription-polymerase chain reaction and immunohistochemistry, respectively, in 60 CRC and paired adjacent non-tumor tissues. NY-ESO-1 antibody was detected in 40 of the 236 (16.9%) patients with CRC. The NY-ESO-1 antibody combined with carcinoembryonic antigen enhanced the sensitivity, from 52.1 to 62.7%, of the diagnosis of CRC. The frequency of antibody positivity increased with the TNM cancer stage (8.8 vs. 28.3% in stages I+II and III+IV, respectively). The mRNA and protein expression levels of NY-ESO-1 were significantly higher in CRC tissue than in adjacent non-tumor tissue. In conclusion, NY-ESO-1 is frequently expressed in CRC with the capacity of inducing a humoral immune response in CRC patients, exhibiting the potential to be a promising biomarker for CRC.

  17. Epigenetic potentiation of NY-ESO-1 vaccine therapy in human ovarian cancer.

    Science.gov (United States)

    Odunsi, Kunle; Matsuzaki, Junko; James, Smitha R; Mhawech-Fauceglia, Paulette; Tsuji, Takemasa; Miller, Austin; Zhang, Wa; Akers, Stacey N; Griffiths, Elizabeth A; Miliotto, Anthony; Beck, Amy; Batt, Carl A; Ritter, Gerd; Lele, Shashikant; Gnjatic, Sacha; Karpf, Adam R

    2014-01-01

    The cancer-testis/cancer-germline antigen NY-ESO-1 is a vaccine target in epithelial ovarian cancer (EOC), but its limited expression is a barrier to vaccine efficacy. As NY-ESO-1 is regulated by DNA methylation, we hypothesized that DNA methyltransferase (DNMT) inhibitors may augment NY-ESO-1 vaccine therapy. In agreement, global DNA hypomethylation in EOC was associated with the presence of circulating antibodies to NY-ESO-1. Pre-clinical studies using EOC cell lines showed that decitabine treatment enhanced both NY-ESO-1 expression and NY-ESO-1-specific CTL-mediated responses. Based on these observations, we performed a phase I dose-escalation trial of decitabine, as an addition to NY-ESO-1 vaccine and doxorubicin liposome (doxorubicin) chemotherapy, in 12 patients with relapsed EOC. The regimen was safe, with limited and clinically manageable toxicities. Both global and promoter-specific DNA hypomethylation occurred in blood and circulating DNAs, the latter of which may reflect tumor cell responses. Increased NY-ESO-1 serum antibodies and T cell responses were observed in the majority of patients, and antibody spreading to additional tumor antigens was also observed. Finally, disease stabilization or partial clinical response occurred in 6/10 evaluable patients. Based on these encouraging results, evaluation of similar combinatorial chemo-immunotherapy regimens in EOC and other tumor types is warranted.

  18. ESO and Fokker Space Sign Contract about VLTI Delay Line

    Science.gov (United States)

    1998-03-01

    The European Southern Observatory is building the world's largest optical telescope, the Very Large Telescope (VLT) , at the ESO Paranal Observatory in Chile. The VLT consists of four 8.2-m unit telescopes and several smaller, moveable Auxiliary Telescopes. When coupled as the giant VLT Interferometer (VLTI) , they will together provide the sharpest images ever obtained by any optical telescope. It will in principle be able to see an astronaut on the surface of the Moon, 400,000 km away. The VLTI Delay Lines Fokker Space (Leiden, The Netherlands) has been awarded a contract for the delivery of the Delay Line of the VLTI. This is a mechanical-optical system that will compensate the optical path differences of the light beams from the individual telescopes. Such a system is necessary to ensure that the light from all telescopes arrive in the same phase at the focal point of the interferometer. Otherwise, the very sharp interferometric images cannot be obtained. ESO PR Photo 08/98 [JPEG, 102k] Schematic representation of the VLTI Delay Line, showing the retro-reflector on its moving base. For more details, please consult the technical explanation below. This highly accurate system will be developed in close co-operation with the Dutch institute TNO-TPD (Netherlands Organization for Applied Scientific Research - Institute of Applied Physics) . The most innovative feature of the Delay Line is the new control strategy, a two-stage control system, based on linear motor technology, combined with high accuracy piezo-electric control elements. This enables the system to position the so-called cat's eye reflector system with an accuracy of only a few nanometers (millionth of a millimetre (nm)) over a stroke length of 60 metres. Within radio astronomy, interferometric techniques have been applied by Dutch astronomers since many years. They will now be able to contribute with their extensive knowledge of such systems to the next generation of astronomical interferometric

  19. Antibody responses to NY-ESO-1 in primary breast cancer identify a subtype target for immunotherapy.

    Science.gov (United States)

    Hamaï, Ahmed; Duperrier-Amouriaux, Karine; Pignon, Pascale; Raimbaud, Isabelle; Memeo, Lorenzo; Colarossi, Cristina; Canzonieri, Vincenzo; Perin, Tiziana; Classe, Jean-Marc; Campone, Mario; Jézéquel, Pascal; Campion, Loïc; Ayyoub, Maha; Valmori, Danila

    2011-01-01

    The highly immunogenic human tumor antigen NY-ESO-1 (ESO) is a target of choice for anti-cancer immune therapy. In this study, we assessed spontaneous antibody (Ab) responses to ESO in a large cohort of patients with primary breast cancer (BC) and addressed the correlation between the presence of anti-ESO Ab, the expression of ESO in the tumors and their characteristics. We found detectable Ab responses to ESO in 1% of the patients. Tumors from patients with circulating Ab to ESO exhibited common characteristics, being mainly hormone receptor (HR)⁻ invasive ductal carcinomas of high grade, including both HER2⁻ and HER2⁺ tumors. In line with these results, we detected ESO expression in 20% of primary HR⁻ BC, including both ESO Ab⁺ and Ab⁻ patients, but not in HR⁺ BC. Interestingly, whereas expression levels in ESO⁺ BC were not significantly different between ESO Ab⁺ and Ab⁻ patients, the former had, in average, significantly higher numbers of tumor-infiltrated lymph nodes, indicating that lymph node invasion may be required for the development of spontaneous anti-tumor immune responses. Thus, the presence of ESO Ab identifies a tumor subtype of HR⁻ (HER2⁻ or HER2⁺) primary BC with frequent ESO expression and, together with the assessment of antigen expression in the tumor, may be instrumental for the selection of patients for whom ESO-based immunotherapy may complement standard therapy.

  20. NY-ESO-1 expression is tightly linked to TMPRSS2-ERG fusion in prostate cancer.

    Science.gov (United States)

    Grupp, Katharina; Ospina-Klinck, Daniel; Tsourlakis, Maria Christina; Koop, Christina; Wilczak, Waldemar; Adam, Meike; Simon, Ronald; Sauter, Guido; Izbicki, Jakob Robert; Graefen, Markus; Huland, Hartwig; Steurer, Stefan; Schlomm, Thorsten; Minner, Sarah; Quaas, Alexander

    2014-07-01

    NY-ESO-1 has been suggested as therapeutic cancer vaccine in prostate cancer. This study was undertaken to explore the relationship of NY-ESO-1 with tumor phenotype, biochemical recurrence, and molecular subgroups in hormone-naive prostate cancers. NY-ESO-1 immunohistochemistry was analyzed on a tissue microarray containing 11,152 prostate cancer samples. Results were compared to clinically follow-up data, ERG status, and deletions on PTEN, 3p13, 5q21, and 6q15. NY-ESO-1 expression was absent in benign prostate glands. In prostate cancer, NY-ESO-1 positivity was found 8.8% of our 8,761 interpretable tumors including 5.8% with weak, 2.5% with moderate, and 0.5% with strong expression. There was a threefold higher rate of NY-ESO-1 expression in ERG fusion positive tumors than in ERG negative cancers (P ESO-1 expression was associated with early biochemical recurrence (P = 0.0002) and high Gleason grade (P ESO-1 expression was also linked to PTEN (P = 0.0012) and 6q15 deletions (P = 0.0005). Our observations indicate a tight link of NY-ESO-1 expression to ERG activation and (to a lesser extent) PTEN- and 6q15-deletions in prostate cancer. The impact of these interactions on the likelihood of response to immunotherapy is unclear. The prognostic impact of NY-ESO-1 expression is little and not independent of histologic variables. © 2014 Wiley Periodicals, Inc.

  1. The Gaia-ESO Survey: Churning through the Milky Way

    Science.gov (United States)

    Hayden, M. R.; Recio-Blanco, A.; de Laverny, P.; Mikolaitis, S.; Guiglion, G.; Hill, V.; Gilmore, G.; Randich, S.; Bayo, A.; Bensby, T.; Bergemann, M.; Bragaglia, A.; Casey, A.; Costado, M.; Feltzing, S.; Franciosini, E.; Hourihane, A.; Jofre, P.; Koposov, S.; Kordopatis, G.; Lanzafame, A.; Lardo, C.; Lewis, J.; Lind, K.; Magrini, L.; Monaco, L.; Morbidelli, L.; Pancino, E.; Sacco, G.; Stonkute, E.; Worley, C. C.; Zwitter, T.

    2018-01-01

    Context. There have been conflicting results with respect to the extent that radial migration has played in the evolution of the Galaxy. Additionally, observations of the solar neighborhood have shown evidence of a merger in the past history of the Milky Way that drives enhanced radial migration. Aims: We attempt to determine the relative fraction of stars that have undergone significant radial migration by studying the orbital properties of metal-rich ([Fe/H] > 0.1) stars within 2 kpc of the Sun. We also aim to investigate the kinematic properties, such as velocity dispersion and orbital parameters, of stellar populations near the Sun as a function of [Mg/Fe] and [Fe/H], which could show evidence of a major merger in the past history of the Milky Way. Methods: We used a sample of more than 3000 stars selected from the fourth internal data release of the Gaia-ESO Survey. We used the stellar parameters from the Gaia-ESO Survey along with proper motions from PPMXL to determine distances, kinematics, and orbital properties for these stars to analyze the chemodynamic properties of stellar populations near the Sun. Results: Analyzing the kinematics of the most metal-rich stars ([Fe/H] > 0.1), we find that more than half have small eccentricities (e 7 kpc. We find that the highest [Mg/Fe], metal-poor populations have lower vertical and radial velocity dispersions compared to lower [Mg/Fe] populations of similar metallicity by 10 km s-1. The median eccentricity increases linearly with [Mg/Fe] across all metallicities, while the perigalacticon decreases with increasing [Mg/Fe] for all metallicities. Finally, the most [Mg/Fe]-rich stars are found to have significant asymmetric drift and rotate more than 40 km s-1 slower than stars with lower [Mg/Fe] ratios. Conclusions: While our results cannot constrain how far stars have migrated, we propose that migration processes are likely to have played an important role in the evolution of the Milky Way, with metal-rich stars

  2. NY-ESO-1 expression in hepatocellular carcinoma: A potential new marker for early recurrence after surgery.

    Science.gov (United States)

    Xu, Heng; Gu, Na; Liu, Zhao-Bo; Zheng, Min; Xiong, Fang; Wang, Si-Ying; Li, Ning; Lu, Jun

    2012-01-01

    NY-ESO-1 belongs to the cancer testis antigens (CTA) family, and is identified in a variety of tumors. Certain studies have demonstrated that NY-ESO-1 predicts tumor recurrence and treatment response. No reports are currently available regarding the correlation between NY-ESO-1 and the recurrence of hepatocellular carcinoma (HCC) following surgery. The purpose of the present study was to evaluate the association between NY-ESO-1 and relapse of HCC and to explore the possible mechanisms for this correlation. A total of 120 HCC patients were analyzed for the expression of NY-ESO-1 by immunohistochemistry (IHC). A stable NY-ESO-1 over-expressed HepG2 cell line (ESO-HepG2) was established to determine the biological effects of NY-ESO-1 on cell proliferation, cell cycle and migration by using the xCELLigence DP system, flow cytometry and xCELLigence SP system. NY-ESO-1 was positive in 28 of 120 (23.3%) HCC tumor tissues. NY-ESO-1 was not detectable in adjacent normal liver tissues. A close correlation was found between NY-ESO-1 expression and the recurrence of HCC following surgery (P=0.007). Kaplan-Meier analysis showed a shorter recurrence-free survival (RFS) for patients positive for NY-ESO-1 (log-rank test, P=0.003). The Cox regression model demonstrated that NY-ESO-1 expression was a significant independent predictor for the recurrence of HCC following curative surgery (P=0.022). Compared with HepG2 cells, ESO-HepG2 cells have increased migration but not proliferation ability. In conclusion, NY-ESO-1 expression is associated with worse HCC outcome following surgery, and the mechanism for this finding may be that NY-ESO-1 increases tumor cell migration.

  3. Gender Systematics in Telescope Time Allocation at ESO

    Science.gov (United States)

    Patat, F.

    2016-09-01

    The results of a comprehensive statistical analysis of gender systematics in the time allocation process at ESO are presented. The sample on which the study is based includes more than 13 000 Normal and Short proposals, submitted by about 3000 principal investigators (PI) over eight years. The genders of PIs, and of the panel members of the Observing Programmes Committee (OPC), were used, together with their career level, to analyse the grade distributions and the proposal success rates. Proposals submitted by female PIs show a significantly lower probability of being allocated time. The proposal success rates (defined as number of top ranked runs over requested runs) are 16.0 ± 0.6% and 22.0 ± 0.4% for females and males, respectively. To a significant extent the disparity is related to different input distributions in terms of career level. The seniority of male PIs is significantly higher than that of female PIs, with only 34% of the female PIs being professionally employed astronomers (compared to 53% for male PIs). A small, but statistically significant, gender-dependent behaviour is measured for the OPC referees: both genders show the same systematics, but they are larger for males than females. The PI female/male fraction is very close to 30/70; although far from parity, the fraction is higher than that observed, for instance, among IAU membership.

  4. Upgrade of ESO's FIERA CCD Controller and PULPO Subsystem

    Science.gov (United States)

    Reyes-Moreno, J.; Geimer, C.; Balestra, A.; Haddad, N.

    An overview of FIERA is presented with emphasis on its recent upgrade to PCI. The PCI board hosts two DSPs, one for real time control of the camera and another for on-the-fly processing of the incoming video data. In addition, the board is able to make DMA transfers, to synchronize to other boards alike, to be synchronized by a TIM bus and to control PULPO via RS232. The design is based on the IOP480 chip from PLX, for which we have developed a device driver for both Solaris and Linux. One computer is able to host more than one board and therefore can control an array of FIERA detector electronics. PULPO is a multifunctional subsystem widely used at ESO for the housekeeping of CCD cryostat heads and for shutter control. The upgrade of PULPO is based on an embedded PC running Linux. The upgraded PULPO is able to handle 29 temperature sensors, control 8 heaters and one shutter, read out one vacuum sensor and log any combination of parameters.

  5. The ESO Diffuse Interstellar Band Large Exploration Survey (EDIBLES)

    Science.gov (United States)

    Cami, J.; Cox, N. L.; Farhang, A.; Smoker, J.; Elyajouri, M.; Lallement, R.; Bacalla, X.; Bhatt, N. H.; Bron, E.; Cordiner, M. A.; de Koter, A..; Ehrenfreund, P.; Evans, C.; Foing, B. H.; Javadi, A.; Joblin, C.; Kaper, L.; Khosroshahi, H. G.; Laverick, M.; Le Petit, F..; Linnartz, H.; Marshall, C. C.; Monreal-Ibero, A.; Mulas, G.; Roueff, E.; Royer, P.; Salama, F.; Sarre, P. J.; Smith, K. T.; Spaans, M.; van Loon, J. T..; Wade, G.

    2018-03-01

    The ESO Diffuse Interstellar Band Large Exploration Survey (EDIBLES) is a Large Programme that is collecting high-signal-to-noise (S/N) spectra with UVES of a large sample of O and B-type stars covering a large spectral range. The goal of the programme is to extract a unique sample of high-quality interstellar spectra from these data, representing different physical and chemical environments, and to characterise these environments in great detail. An important component of interstellar spectra is the diffuse interstellar bands (DIBs), a set of hundreds of unidentified interstellar absorption lines. With the detailed line-of-sight information and the high-quality spectra, EDIBLES will derive strong constraints on the potential DIB carrier molecules. EDIBLES will thus guide the laboratory experiments necessary to identify these interstellar “mystery molecules”, and turn DIBs into powerful diagnostics of their environments in our Milky Way Galaxy and beyond. We present some preliminary results showing the unique capabilities of the EDIBLES programme.

  6. T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.

    Science.gov (United States)

    Singh, Nathan; Kulikovskaya, Irina; Barrett, David M; Binder-Scholl, Gwendolyn; Jakobsen, Bent; Martinez, Daniel; Pawel, Bruce; June, Carl H; Kalos, Michael D; Grupp, Stephan A

    The cancer-testis antigen NY-ESO-1 is expressed by many solid tumors and has limited expression by mature somatic tissues, making it a highly attractive target for tumor immunotherapy. Targeting NY-ESO-1 using engineered T cells has demonstrated clinical efficacy in the treatment of some adult tumors. Neuroblastoma is a significant cause of cancer mortality in children, and is a tumor type shown to be responsive to immunotherapies. We evaluated a large panel of primarily resected neuroblastoma samples and demonstrated that 23% express NY-ESO-1. After confirming antigen-specific activity of T cells genetically engineered to express an NY-ESO-1 directed high-affinity transgenic T cell receptor in vitro , we performed xenograft mouse studies assessing the efficacy of NY-ESO-1-targeted T cells in both localized and disseminated models of neuroblastoma. Disease responses were monitored by tumor volume measurement and in vivo bioluminescence. After delivery of NY-ESO-1 transgenic TCR T cells, we observed significant delay of tumor progression in mice bearing localized and disseminated neuroblastoma, as well as enhanced animal survival. These data demonstrate that NY-ESO-1 is an antigen target in neuroblastoma and that targeted T cells represent a potential therapeutic option for patients with neuroblastoma.

  7. Expression of MAGE-A and NY-ESO-1 in Primary and Metastatic Cancers.

    Science.gov (United States)

    Park, Tristen S; Groh, Eric M; Patel, Krishna; Kerkar, Sid P; Lee, Chyi-Chia Richard; Rosenberg, Steven A

    2016-01-01

    Melanoma-associated antigen-A (MAGE-A) and New York esophageal squamous cell cancer-1 (NY-ESO-1) are 2 cancer testis antigens (CTA) demonstrating potential for use in targeted immunotherapy. Clinical trials in melanoma and synovial sarcomas targeting these antigens in immune-based therapies have demonstrated durable tumor regression. Although protein expression of NY-ESO-1 has been assessed in a variety of cancer types, the expression of MAGE-A has not been studied in depth. In this study we analyzed MAGE-A and NY-ESO-1 expression in 314 melanoma specimens from 301 melanoma patients, 38 patients with squamous cell cancers and 111 patients with adenocarcinomas. Our results demonstrated higher expression of MAGE-A compared with NY-ESO-1 in melanomas (32% vs. 13%) and squamous cell carcinomas (45% vs. 7.9%), and higher expression of both CTAs in metastatic versus primary tumors. CTA expression in adenocarcinomas was low (MAGE-A: 10%, NY-ESO-1: 0.9%). In addition, we looked at concordance of expression among metastatic melanoma lesions within the same patient and found concordant expression in 38 of 47 patients for MAGE-A and 43 of 47 patients for NY-ESO-1. Our study demonstrated that the MAGE-A family may be of greater utility than NY-ESO-1 for targeted immunotherapy in a variety of cancer histologies, in particular metastatic melanomas and squamous cell carcinomas.

  8. VizieR Online Data Catalog: KiDS-ESO-DR3 multi-band source catalog (de Jong+, 2017)

    NARCIS (Netherlands)

    de Jong, J. T. A.; Verdoes Kleijn, G. A.; Erben, T.; Hildebrandt, H.; Kuijken, K.; Sikkema, G.; Brescia, M.; Bilicki, M.; Napolitano, N. R.; Amaro, V.; Begeman, K. G.; Boxhoorn, D. R.; Buddelmeijer, H.; Cavuoti, S.; Getman, F.; Grado, A.; Helmich, E.; Huang, Z.; Irisarri, N.; La Barbera, F.; Longo, G.; McFarland, J. P.; Nakajima, R.; Paolillo, M.; Puddu, E.; Radovich, M.; Rifatto, A.; Tortora, C; Valentijn, E. A.; Vellucci, C.; Vriend, W-J.; Amon, A.; Blake, C.; Choi, A.; Fenech Conti, I.; Herbonnet, R.; Heymans, C.; Hoekstra, H.; Klaes, D.; Merten, J.; Miller, L.; Schneider, P.; Viola, M.

    2017-01-01

    KiDS-ESO-DR3 contains a multi-band source catalogue encompassing all publicly released tiles, a total of 440 survey tiles including the coadded images, weight maps, masks and source lists of 292 survey tiles of KiDS-ESO-DR3, adding to the 148 tiles released previously (50 in KiDS-ESO-DR1 and 98 in

  9. Mechanical thrombectomy in acute ischemic stroke: Consensus statement by ESO-Karolinska Stroke Update 2014/2015, supported by ESO, ESMINT, ESNR and EAN.

    Science.gov (United States)

    Wahlgren, Nils; Moreira, Tiago; Michel, Patrik; Steiner, Thorsten; Jansen, Olav; Cognard, Christophe; Mattle, Heinrich P; van Zwam, Wim; Holmin, Staffan; Tatlisumak, Turgut; Petersson, Jesper; Caso, Valeria; Hacke, Werner; Mazighi, Mikael; Arnold, Marcel; Fischer, Urs; Szikora, Istvan; Pierot, Laurent; Fiehler, Jens; Gralla, Jan; Fazekas, Franz; Lees, Kennedy R

    2016-01-01

    The original version of this consensus statement on mechanical thrombectomy was approved at the European Stroke Organisation (ESO)-Karolinska Stroke Update conference in Stockholm, 16-18 November 2014. The statement has later, during 2015, been updated with new clinical trials data in accordance with a decision made at the conference. Revisions have been made at a face-to-face meeting during the ESO Winter School in Berne in February, through email exchanges and the final version has then been approved by each society. The recommendations are identical to the original version with evidence level upgraded by 20 February 2015 and confirmed by 15 May 2015. The purpose of the ESO-Karolinska Stroke Update meetings is to provide updates on recent stroke therapy research and to discuss how the results may be implemented into clinical routine. Selected topics are discussed at consensus sessions, for which a consensus statement is prepared and discussed by the participants at the meeting. The statements are advisory to the ESO guidelines committee. This consensus statement includes recommendations on mechanical thrombectomy after acute stroke. The statement is supported by ESO, European Society of Minimally Invasive Neurological Therapy (ESMINT), European Society of Neuroradiology (ESNR), and European Academy of Neurology (EAN). © 2016 World Stroke Organization.

  10. A novel human-derived antibody against NY-ESO-1 improves the efficacy of chemotherapy.

    Science.gov (United States)

    Gupta, Anurag; Nuber, Natko; Esslinger, Christoph; Wittenbrink, Mareike; Treder, Martin; Landshammer, Alexandro; Noguchi, Takuro; Kelly, Marcus; Gnjatic, Sacha; Ritter, Erika; von Boehmer, Lotta; Nishikawa, Hiroyoshi; Shiku, Hiroshi; Old, Lloyd; Ritter, Gerd; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    We investigated whether antibodies against intracellular tumor-associated antigens support tumor-specific immunity when administered together with a treatment that destroys the tumor. We propose that released antigens form immune complexes with the antibodies, which are then efficiently taken up by dendritic cells. We cloned the first human monoclonal antibodies against the Cancer/Testis (CT) antigen, NY-ESO-1. We tested whether the monoclonal anti-NY-ESO-1 antibody (12D7) facilitates cross-presentation of a NY-ESO-1-derived epitope by dendritic cells to human CD8+ T cells, and whether this results in the maturation of dendritic cells in vitro. We investigated the efficacy of 12D7 in combination with chemotherapy using BALB/c mice bearing syngeneic CT26 tumors that express intracellular NY-ESO-1. Human dendritic cells that were incubated with NY-ESO-1:12D7 immune complexes efficiently stimulated NY-ESO-1(157-165)/HLA-A2-specific human CD8+ T cells to produce interferon-γ, whereas NY-ESO-1 alone did not. Furthermore, the incubation of dendritic cells with NY-ESO-1:12D7 immune complexes resulted in the maturation of dendritic cells. Treatment of BALB/c mice that bear CT26/NY-ESO-1 tumors with 5-fluorouracil (5-FU) plus 12D7 was significantly more effective than chemotherapy alone. We propose systemic injection of monoclonal antibodies (mAbs) against tumor-associated antigens plus a treatment that promotes the local release of those antigens resulting in immune complex formation as a novel therapeutic modality for cancer.

  11. Recommendations from the ESO-Karolinska Stroke Update Conference, Stockholm 13–15 November 2016

    DEFF Research Database (Denmark)

    2017-01-01

    About the meeting: The purpose of the European Stroke Organisation (ESO)-Karolinska Stroke Update Conference is to provide updates on recent stroke therapy research and to give an opportunity for the participants to discuss how these results may be implemented into clinical routine. Several......://www.eso-karolinska.org/2016 and http://eso-stroke.org) and recommendations which were prepared by a writing committee consisting of session chair(s), secretary and speakers and presented to the 312 participants of the meeting. In the open meeting, general participants commented on the consensus statement and recommendations...

  12. Automated data reduction workflows for astronomy. The ESO Reflex environment

    Science.gov (United States)

    Freudling, W.; Romaniello, M.; Bramich, D. M.; Ballester, P.; Forchi, V.; García-Dabló, C. E.; Moehler, S.; Neeser, M. J.

    2013-11-01

    Context. Data from complex modern astronomical instruments often consist of a large number of different science and calibration files, and their reduction requires a variety of software tools. The execution chain of the tools represents a complex workflow that needs to be tuned and supervised, often by individual researchers that are not necessarily experts for any specific instrument. Aims: The efficiency of data reduction can be improved by using automatic workflows to organise data and execute a sequence of data reduction steps. To realize such efficiency gains, we designed a system that allows intuitive representation, execution and modification of the data reduction workflow, and has facilities for inspection and interaction with the data. Methods: The European Southern Observatory (ESO) has developed Reflex, an environment to automate data reduction workflows. Reflex is implemented as a package of customized components for the Kepler workflow engine. Kepler provides the graphical user interface to create an executable flowchart-like representation of the data reduction process. Key features of Reflex are a rule-based data organiser, infrastructure to re-use results, thorough book-keeping, data progeny tracking, interactive user interfaces, and a novel concept to exploit information created during data organisation for the workflow execution. Results: Automated workflows can greatly increase the efficiency of astronomical data reduction. In Reflex, workflows can be run non-interactively as a first step. Subsequent optimization can then be carried out while transparently re-using all unchanged intermediate products. We found that such workflows enable the reduction of complex data by non-expert users and minimizes mistakes due to book-keeping errors. Conclusions: Reflex includes novel concepts to increase the efficiency of astronomical data processing. While Reflex is a specific implementation of astronomical scientific workflows within the Kepler workflow

  13. Detection of spectroscopic binaries in the Gaia-ESO Survey

    Science.gov (United States)

    Van der Swaelmen, M.; Merle, T.; Van Eck, S.; Jorissen, A.

    2017-12-01

    The Gaia-ESO survey (GES) is a ground-based spectroscopic survey, complementing the Gaia mission, in order to obtain high accuracy radial velocities and chemical abundances for 10^5 stars. Thanks to the numerous spectra collected by the GES, the detection of spectroscopic multiple system candidates (SBn, n ≥ 2) is one of the science case that can be tackled. We developed at IAA (Institut d'Astronomie et d'Astrophysique) a novative automatic method to detect multiple components from the cross-correlation function (CCF) of spectra and applied it to the CCFs provided by the GES. Since the bulk of the Milky Way field targets has been observed in both HR10 and HR21 GIRAFFE settings, we are also able to compare the efficiency of our SB detection tool depending on the wavelength range. In particular, we show that HR21 leads to a less efficient detection compared to HR10. The presence of strong and/or saturated lines (Ca II triplet, Mg I line, Paschen lines) in the wavelength domain covered by HR21 hampers the computation of CCFs, which tend to be broadened compared to their HR10 counterpart. The main drawback is that the minimal detectable radial velocity difference is ˜ \\SI{60}km/s for HR21 while it is ˜ \\SI{25}km/s for HR10. A careful design of CCF masks (especially masking Ca triplet lines) can substantially improve the detectability rate of HR21. Since HR21 spectra are quite similar to the one produced by the RVS spectrograph of the Gaia mission, analysis of RVS spectra in the context of spectroscpic binaries can take adavantage of the lessons learned from the GES to maximize the detection rate.

  14. ESO NTT IRSPEC IMAGE DATA FROM SL9 IMPACTS WITH JUPITER V1.0

    Data.gov (United States)

    National Aeronautics and Space Administration — At the ESO La Silla site is a direct imaging facility (DIFA) at the A Nasmyth focus of the NTT, in front of the image derotator which feeds the IRSPEC infrared...

  15. ESO NTT SUSI IMAGE DATA FROM SL9 IMPACTS WITH JUPITER V1.0

    Data.gov (United States)

    National Aeronautics and Space Administration — At the ESO La Silla site is a direct imaging facility (DIFA) at the A Nasmyth focus of the NTT, in front of the image derotator which feeds the IRSPEC infrared...

  16. NY-ESO-1 as a potential immunotherapeutic target in renal cell carcinoma.

    Science.gov (United States)

    Giesen, Eva; Jilaveanu, Lucia B; Parisi, Fabio; Kluger, Yuval; Camp, Robert L; Kluger, Harriet M

    2014-07-30

    Novel immune therapies targeting tumor specific antigens are being developed. Our purpose was to determine expression of the cancer testes antigen NY-ESO-1 in renal cell carcinoma (RCC), as NY-ESO-1 targeting approaches, particularly adoptive cell therapy, have not been evaluated in this disease. We employed tissue microarrays containing >300 unique RCC cases and adjacent benign renal tissue to determine NY-ESO-1 expression using a quantitative immunofluorescence method. In addition, we studied NY-ESO-1 expression in 35 matched primary and metastatic RCC specimens to assess concordance between different tumor sites. NY-ESO-1 was highly expressed in a subset of RCCs. Expression in primary RCC specimens was significantly higher than adjacent normal renal tissue (PESO-1 expression seen in clear cell RCC suggests that NY-ESO-1 targeting approaches should be studied in this disease. Expression is higher in metastatic sites, and discordance between primary and metastatic sites in some patients suggests that patient selection for these therapies should be based on expression in metastatic rather than nephrectomy specimens.

  17. NY-ESO-1 (CTAG1B) expression in mesenchymal tumors.

    Science.gov (United States)

    Endo, Makoto; de Graaff, Marieke A; Ingram, Davis R; Lim, Simin; Lev, Dina C; Briaire-de Bruijn, Inge H; Somaiah, Neeta; Bovée, Judith V M G; Lazar, Alexander J; Nielsen, Torsten O

    2015-04-01

    New York esophageal squamous cell carcinoma 1 (NY-ESO-1, CTAG1B) is a cancer-testis antigen and currently a focus of several targeted immunotherapeutic strategies. We performed a large-scale immunohistochemical expression study of NY-ESO-1 using tissue microarrays of mesenchymal tumors from three institutions in an international collaboration. A total of 1132 intermediate and malignant and 175 benign mesenchymal lesions were enrolled in this study. Immunohistochemical staining was performed on tissue microarrays using a monoclonal antibody for NY-ESO-1. Among mesenchymal tumors, myxoid liposarcomas showed the highest positivity for NY-ESO-1 (88%), followed by synovial sarcomas (49%), myxofibrosarcomas (35%), and conventional chondrosarcomas (28%). Positivity of NY-ESO-1 in the remaining mesenchymal tumors was consistently low, and no immunoreactivity was observed in benign mesenchymal lesions. On the basis of these findings, nearly 90% of myxoid liposarcomas, as well as a significant proportion of synovial sarcomas, myxofibrosarcomas, and conventional chondrosarcomas are good candidates for immunotherapy targeting NY-ESO-1.

  18. Three novel NY-ESO-1 epitopes bound to DRB1*0803, DQB1*0401 and DRB1*0901 recognized by CD4 T cells from CHP-NY-ESO-1-vaccinated patients.

    Science.gov (United States)

    Mizote, Yu; Taniguchi, Taku; Tanaka, Kei; Isobe, Midori; Wada, Hisashi; Saika, Takashi; Kita, Shoichi; Koide, Yukari; Uenaka, Akiko; Nakayama, Eiichi

    2010-07-19

    Three novel NY-ESO-1 CD4 T cell epitopes were identified using PBMC obtained from patients who were vaccinated with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein (CHP-NY-ESO-1). The restriction molecules were determined by antibody blocking and using various EBV-B cells with different HLA alleles as APC to present peptides to CD4 T cells. The minimal epitope peptides were determined using various N- and C-termini truncated peptides deduced from 18-mer overlapping peptides originally identified for recognition. Those epitopes were DRB1*0901-restricted NY-ESO-1 87-100, DQB1*0401-restricted NY-ESO-1 95-107 and DRB1*0803-restricted NY-ESO-1 124-134. CD4 T cells used to determine those epitope peptides recognized EBV-B cells or DC that were treated with recombinant NY-ESO-1 protein or NY-ESO-1-expressing tumor cell lysate, suggesting that the epitope peptides are naturally processed. These CD4 T cells showed a cytokine profile with Th1 characteristics. Furthermore, NY-ESO-1 87-100 peptide/HLA-DRB1*0901 tetramer staining was observed. Multiple Th1-type CD4 T cell responses are beneficial for inducing effective anti-tumor responses after NY-ESO-1 protein vaccination. (c) 2010 Elsevier Ltd. All rights reserved.

  19. [Prokaryotic expression, purification and identification of NY-ESO-1/GST fusion protein in E.coli].

    Science.gov (United States)

    Tang, Lei; Song, Chao-jun; Sun, Yuan-jie; Li, Na; Wei, Yu-ying; Sun, Yi; Yang, Kun

    2012-10-01

    To construct an expression plasmid for NY-ESO-1 gene and identify the expression of recombinant protein NY-ESO-1/GST in E.coli. NY-ESO-1 segment was amplified from the testis cDNA library by RT-PCR and cloned into the prokaryotic expression vector pGEX4T-1 downstream tagged by GST to construct the expression plasmid pGEX-4T1-NY-ESO-1. The recombinant vector was transformed to BL21 (DE3) and NY-ESO-1/GST fusion protein was induced expression by IPTG. The protein was purified by urea elution and identified by SDS-PAGE and Western blotting. The NY-ESO-1 segment was successfully amplified and its sequence was identical with that published in GenBank. The BL21 (DE3) pLysS containing the pGEX-4T1-NY-ESO-1 expressed a M(r); 44 000 fusion protein under the induction of IPTG. The purity of the protein was 90%. Western blotting proved that NY-ESO-1/GST had a specific reaction with anti-GST mAb. The prokaryotic expression vector of NY-ESO-1 has been constructed and the fusion protein NY-ESO-1/GST of high purity is successfully expressed.

  20. Efficacy of systemic adoptive transfer immunotherapy targeting NY-ESO-1 for glioblastoma.

    Science.gov (United States)

    Everson, Richard G; Antonios, Joseph P; Lisiero, Dominique N; Soto, Horacio; Scharnweber, Rudi; Garrett, Matthew C; Yong, William H; Li, Ning; Li, Gang; Kruse, Carol A; Liau, Linda M; Prins, Robert M

    2016-03-01

    Immunotherapy is an ideal treatment modality to specifically target the diffusely infiltrative tumor cells of malignant gliomas while sparing the normal brain parenchyma. However, progress in the development of these therapies for glioblastoma has been slow due to the lack of immunogenic antigen targets that are expressed uniformly and selectively by gliomas. We utilized human glioblastoma cell cultures to induce expression of New York-esophageal squamous cell carcinoma (NY-ESO-1) following in vitro treatment with the demethylating agent decitabine. We then investigated the phenotype of lymphocytes specific for NY-ESO-1 using flow cytometry analysis and cytotoxicity against cells treated with decitabine using the xCelligence real-time cytotoxicity assay. Finally, we examined the in vivo application of this immune therapy using an intracranially implanted xenograft model for in situ T cell trafficking, survival, and tissue studies. Our studies showed that treatment of intracranial glioma-bearing mice with decitabine reliably and consistently induced the expression of an immunogenic tumor-rejection antigen, NY-ESO-1, specifically in glioma cells and not in normal brain tissue. The upregulation of NY-ESO-1 by intracranial gliomas was associated with the migration of adoptively transferred NY-ESO-1-specific lymphocytes along white matter tracts to these tumors in the brain. Similarly, NY-ESO-1-specific adoptive T cell therapy demonstrated antitumor activity after decitabine treatment and conferred a highly significant survival benefit to mice bearing established intracranial human glioma xenografts. Transfer of NY-ESO-1-specific T cells systemically was superior to intracranial administration and resulted in significantly extended and long-term survival of animals. These results reveal an innovative, clinically feasible strategy for the treatment of glioblastoma. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Neuro

  1. Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination.

    Science.gov (United States)

    Kawada, Junji; Wada, Hisashi; Isobe, Midori; Gnjatic, Sacha; Nishikawa, Hiroyoshi; Jungbluth, Achim A; Okazaki, Nami; Uenaka, Akiko; Nakamura, Yurika; Fujiwara, Shinichi; Mizuno, Naoaki; Saika, Takashi; Ritter, Erika; Yamasaki, Makoto; Miyata, Hiroshi; Ritter, Gerd; Murphy, Roger; Venhaus, Ralph; Pan, Linda; Old, Lloyd J; Doki, Yuichiro; Nakayama, Eiichi

    2012-02-01

    NY-ESO-1 is a prototypic cancer/testis antigen. In a recent phase I clinical trial, we vaccinated 13 patients bearing NY-ESO-1-expressing tumors with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein (CHP-NY-ESO-1) and showed efficient induction of NY-ESO-1 antibody, and CD4 and CD8 T cell responses using peripheral blood from the patients. In our study, we analyzed heteroclitic serological responses in those patients after vaccination. Serological response against 11 tumor antigens including MAGE-A1, MAGE-A3, MAGE-A4, CT7/MAGEC1, CT10/MAGEC2, CT45, CT46/HORMAD1, SOX2, SSX2, XAGE1B and p53 was examined by enzyme-linked immunosorbent assay (ELISA) using sera from ten vaccinated patients. Expression of tumor antigens was determined by reverse transcription-polymerase chain reaction or immunohistochemistry. Eight of nine patients who showed antibody responses against NY-ESO-1 also showed an antibody response against at least 1 of these 11 tumor antigens after vaccination. In one patient, seven tumor antigens were recognized. Specificity analysis of the antibody response by ELISA using control recombinant proteins and synthetic peptides and by Western blot showed that the response was not against His6-tag and/or bacterial products included in a preparation of CHP-NY-ESO-1 used for vaccination. Thus, heteroclitic serological responses appear to be indicative of the overall immune response against the tumor, and their analysis could be useful for immune monitoring in cancer vaccine. Copyright © 2011 UICC.

  2. NY-ESO-1 expression in meningioma suggests a rationale for new immunotherapeutic approaches.

    Science.gov (United States)

    Baia, Gilson S; Caballero, Otavia L; Ho, Janelle S Y; Zhao, Qi; Cohen, Tzeela; Binder, Zev A; Salmasi, Vafi; Gallia, Gary L; Quinones-Hinojosa, Alfredo; Olivi, Alessandro; Brem, Henry; Burger, Peter; Strausberg, Robert L; Simpson, Andrew J G; Eberhart, Charles G; Riggins, Gregory J

    2013-11-01

    Meningiomas are the most common primary intracranial tumors. Surgical resection remains the treatment of choice for these tumors. However, a significant number of tumors are not surgically accessible, recur, or become malignant, necessitating the repetition of surgery and sometimes radiation. Chemotherapy is rarely used and is generally not recognized as an effective treatment. Cancer/testis (CT) genes represent a unique class of genes, which are expressed by germ cells, normally silenced in somatic cells, but activated in various cancers. CT proteins can elicit spontaneous immune responses in patients with cancer and this feature makes them attractive targets for immunotherapy-based approaches. We analyzed mRNA expression of 37 testis-restricted CT genes in a discovery set of 18 meningiomas by reverse transcription PCR. The overall frequency of expression of CT genes ranged from 5.6% to 27.8%. The most frequently expressed was NY-ESO-1, in 5 patients (27.8%). We subsequently analyzed NY-ESO-1 protein expression in a larger set of meningiomas by immunohistochemistry and found expression in 108 of 110 cases. In some cases, NY-ESO-1 expression was diffused and homogenous, but in most instances it was heterogeneous. Importantly, NY-ESO-1 expression was positively correlated with higher grade and patients presenting with higher levels of NY-ESO-1 staining had significantly worse disease-free and overall survival. We have also shown that NY-ESO-1 expression may lead to humoral immune response in patients with meningioma. Considering the limited treatment options for patients with meningioma, the potential of NY-ESO-1-based immunotherapy should be explored. ©2013 AACR.

  3. ESO Telescope Designer Raymond Wilson Wins Prestigious Kavli Award for Astrophysics

    Science.gov (United States)

    2010-06-01

    Raymond Wilson, whose pioneering optics research at ESO made today's giant telescopes possible thanks to "active optics" technology, has been awarded the 2010 Kavli Prize in astrophysics. The founder and original leader of the Optics and Telescopes Group at ESO, Wilson shares the million-dollar prize with two American scientists, Jerry Nelson and Roger Angel. The biennial prize, presented by the Norwegian Academy of Science and Letters, the Kavli Foundation, and the Norwegian Ministry of Education and Research, was instituted in 2008 and is given to researchers who significantly advance knowledge in the fields of nanoscience, neuroscience, and astrophysics, acting as a complement to the Nobel Prize. The award is named for and funded by Fred Kavli, the Norwegian entrepreneur and phi­lanthropist who later founded the Kavlico Corpora­tion in the US - today one of the world's largest suppliers of sensors for aeronautic, automotive and industrial applications. Wilson, who joined ESO in 1972, strived to achieve optical perfection, developing the concept of active optics as a way to enhance the size of telescopic primary mirrors. It is the size of these mirrors that determines the ability of a telescope to gather light and study faint and distant objects. Before active optics, mirrors over six metres in diameter were impossible, being too heavy, costly, and likely to bend from gravity and temperature changes. The use of active optics, which preserves optimal image quality by continually adjusting the mirror's shape during observations, made lighter, thinner so-called "meniscus mirrors" possible. Wilson first led the implementation of active optics in the revolutionary New Technology Telescope at ESO's La Silla Observatory, and continued to develop and improve the technology until his retirement in 1993. Since then, active optics have become a standard part of modern astronomy, applied in every big telescope including ESO's Very Large Telescope (VLT), a telescope array

  4. ESO-Based Fuzzy Sliding-Mode Control for a 3-DOF Serial-Parallel Hybrid Humanoid Arm

    Directory of Open Access Journals (Sweden)

    Yueling Wang

    2014-01-01

    Full Text Available This paper presents a unique ESO-based fuzzy sliding-mode controller (FSMC-ESO for a 3-DOF serial-parallel hybrid humanoid arm (HHA for the trajectory tracking control problem. The dynamic model of the HHA is obtained by Lagrange method and is nonlinear in dynamics with inertia uncertainty and external disturbance. The FSMC-ESO is based on the combination of the sliding-mode control (SMC, extended state observer (ESO theory, and fuzzy control (FC. The SMC is insensitive to both internal parameter uncertainties and external disturbances. The motivation for using ESO is to estimate the disturbance in real-time. The fuzzy parameter self-tuning strategy is proposed to adjust the switching gain on line according to the running state of the system. The stability of the system is guaranteed in the sense of the Lyapunov stability theorem. The effectiveness and robustness of the designed FSMC-ESO are illustrated by simulations.

  5. Radio-continuum jets around the peculiar galaxy pair ESO 295-IG022

    Directory of Open Access Journals (Sweden)

    Filipović M.D.

    2010-01-01

    Full Text Available We report new radio-continuum observations with the Australia Telescope Compact Array (ATCA of the region surrounding the peculiar galaxy pair ESO 295-IG022 at the centre of the poor cluster Abell S0102. We observed this cluster at wavelengths of λ=20/13 and 6/3 cm with the ATCA 6 km array. With these configurations, we achieved a resolution of ~2'' at 3 cm which is sufficient to resolve the jet-like structure of ~3' length detected at 20 cm. From our new high resolution images at 6 and 3 cm we confirm the presence of a double jet structure, most likely originating from the northern galaxy (ESO 295-IG022-N, bent and twisted towards the south. We found the spectral index of the jet to be very steep (α=-1.32. No point source was detected that could be associated with the core of ESO 295-IG022-N. On the other hand, ESO 295-IG022-S does not show any jet structure, but does show a point radio source. This source has variable flux and spectral index, and appears to be superposed on the line-of-sight of the jets (seen at 20-cm originating from the northern galaxy ESO 295-IG022-N. Finally, regions of very high and somewhat well ordered polarization were detected at the level of 70%.

  6. REOSC Delivers the Best Astronomical Mirror in the World to ESO

    Science.gov (United States)

    1999-12-01

    On December 14, 1999, REOSC , the Optical Department of the SAGEM Group , finished the polishing of the fourth 8.2-m main mirror for the Very Large Telescope (VLT) of the European Southern Observatory. The mirror was today delivered to ESO at a ceremony at the REOSC factory in Saint Pierre du Perray, just south of Paris. The precision of the form of the mirror that was achieved during the polishing process is 8.5 nanometer (1 nanometer = 1 millionth of a millimetre) over the optical surface. This exceptional value corresponds to an optical resolution (theoretical image sharpness) of 0.03 arcseconds in the visible spectrum. This corresponds to distinguishing two objects separated by only 15 cm at a distance of 1000 km and will allow to detect astronomical objects that are 10,000 million times fainter than what can be perceived with the unaided eye. This impressive measure of quality, achieved by the REOSC teams during much painstaking work, implies that this VLT mirror is the most accurate in the world. In fact, all four 8.2-m VLT main mirrors polished by REOSC are well within the very strict specifications set by ESO, but this is the best of them all. The celebration today is the successful highlight of a contract initiated more than ten years ago, during which REOSC has perfected new polishing and control techniques - innovations improved and developed in a unique workshop dedicated to these giant mirrors. These methods and means are directly applicable to the new generations of segmented mirrors that are now being developed for astronomy and space observations. They are, in this sense, at the foremost front of optical technology. REOSC, the Optical Department of the SAGEM Group , is specialised in the study and realisation of high-precision optics for astronomy, space, defence, science and industry. For earlier information about the work on the VLT mirrors, cf. ESO Press Release 15/95 (13 November 1995). The SAGEM Group is a French high-technology group. It

  7. Radio-Continuum Jets Around the Peculiar Galaxy Pair ESO 295-IG022

    Directory of Open Access Journals (Sweden)

    Filipovic, M. D.

    2010-12-01

    Full Text Available We report new radio-continuum observations with the Australia Telescope Compact Array (ATCA of the region surrounding the peculiar galaxy pair ESO 295-IG022 at the centre of the poor cluster Abell S0102. We observed this cluster at wavelengths of $lambda$=20/13 and 6/3~cm with the ATCA 6~km array. With these configurations, we achieved a resolution of $sim$2arcsec at 3~cm which is sufficient to resolve the jet-like structure of $sim$3 arcmin lengthdetected at 20~cm. From our new high resolution images at 6 and 3~cm we confirm the presence of a double jet structure, most likely originating from the northern galaxy (ESO 295-IG022-N, bent and twisted towards the south. We found the spectral index of the jet to be very steep ($alpha$=-1.32. No point source was detected that could be associated with the core of ESO 295-IG022-N. On the other hand, ESO 295-IG022-S does not show any jet structure, but does show a point radio source. This source has variable flux and spectral index, and appears to be superposed on the line-of-sight of the jets (seen at 20-cm originating from the northern galaxy ESO 295-IG022-N. Finally, regions of very high and somewhat well ordered polarisation were detected at the level of 70 per cent.

  8. Europe to the stars ESO's first 50 years of exploring the southern sky

    CERN Document Server

    Schilling, Govert

    2012-01-01

    The creation of the European Southern Observatory (ESO) in 1962 was the culmination of the dream of leading astronomers from five European countries. Over the years, as more member states joined, ESO constructed the La Silla and Paranal observatories, as well as the Atacama Large Millimeter/submillimeter Array (ALMA) together with partners. ESO is now starting to build the world′s biggest eye on the sky, the European Extremely Large Telescope. At the dawn of 2012, its 50th anniversary year, ESO is ready to enter a new era. One that not even its founding members could have anticipated in their boldest dreams. Constantly at the technological forefront, ESO is ready to tackle new and as yet unimaginable territories of high–precision technology and scientific discovery. Produced especially for ESO′s 50th anniversary, this sumptuously illustrated book takes the reader behind the scenes of the most productive ground–based observatory in the world. It contains the best 300 of ESO′s images, hand–picked fr...

  9. A phase I study of vaccination with NY-ESO-1f peptide mixed with Picibanil OK-432 and Montanide ISA-51 in patients with cancers expressing the NY-ESO-1 antigen.

    Science.gov (United States)

    Kakimi, Kazuhiro; Isobe, Midori; Uenaka, Akiko; Wada, Hisashi; Sato, Eiichi; Doki, Yuichiro; Nakajima, Jun; Seto, Yasuyuki; Yamatsuji, Tomoki; Naomoto, Yoshio; Shiraishi, Kenshiro; Takigawa, Nagio; Kiura, Katsuyuki; Tsuji, Kazuhide; Iwatsuki, Keiji; Oka, Mikio; Pan, Linda; Hoffman, Eric W; Old, Lloyd J; Nakayama, Eiichi

    2011-12-15

    We conducted a phase I clinical trial of a cancer vaccine using a 20-mer NY-ESO-1f peptide (NY-ESO-1 91-110) that includes multiple epitopes recognized by antibodies, and CD4 and CD8 T cells. Ten patients were immunized with 600 μg of NY-ESO-1f peptide mixed with 0.2 KE Picibanil OK-432 and 1.25 ml Montanide ISA-51. Primary end points of the study were safety and immune response. Subcutaneous injection of the NY-ESO-1f peptide vaccine was well tolerated. Vaccine-related adverse events observed were fever (Grade 1), injection-site reaction (Grade 1 or 2) and induration (Grade 2). Vaccination with the NY-ESO-1f peptide resulted in an increase or induction of NY-ESO-1 antibody responses in nine of ten patients. The sera reacted with recombinant NY-ESO-1 whole protein as well as the NY-ESO-1f peptide. An increase in CD4 and CD8 T cell responses was observed in nine of ten patients. Vaccine-induced CD4 and CD8 T cells responded to NY-ESO-1 91-108 in all patients with various HLA types with a less frequent response to neighboring peptides. The findings indicate that the 20-mer NY-ESO-1f peptide includes multiple epitopes recognized by CD4 and CD8 T cells with distinct specificity. Of ten patients, two with lung cancer and one with esophageal cancer showed stable disease. Our study shows that the NY-ESO-1f peptide vaccine was well tolerated and elicited humoral, CD4 and CD8 T cell responses in immunized patients. Copyright © 2011 UICC.

  10. The Results of ESO/SRC Plates Survey of Southern Hemisphere

    Science.gov (United States)

    Gyulbudaghian, A. L.

    2017-07-01

    We have searched the ESO/SRC (E, B, R, J) charts of Southern Hemisphere for discovering of new star forming regions, cometary nebulae, HH objects, tight trapezium like systems, consisting of late type dwarf stars, jets from the stars, and also of radial systems of dark globules. This work was done in several places: in Estonia (Tartu observatory), in Mexico (Mexico, UNAM, Institute of Astronomy), in Chile (twice: ESO Vitacura, Cerro Calan Observatory, Santiago). As a result of this work several dozens of each mentioned above type of objects were found.

  11. Heritability of strabismus: genetic influence is specific to eso-deviation and independent of refractive error.

    Science.gov (United States)

    Sanfilippo, Paul G; Hammond, Christopher J; Staffieri, Sandra E; Kearns, Lisa S; Melissa Liew, S H; Barbour, Julie M; Hewitt, Alex W; Ge, Dongliang; Snieder, Harold; Mackinnon, Jane R; Brown, Shayne A; Lorenz, Birgit; Spector, Tim D; Martin, Nicholas G; Wilmer, Jeremy B; Mackey, David A

    2012-10-01

    Strabismus represents a complex oculomotor disorder characterized by the deviation of one or both eyes and poor vision. A more sophisticated understanding of the genetic liability of strabismus is required to guide searches for associated molecular variants. In this classical twin study of 1,462 twin pairs, we examined the relative influence of genes and environment in comitant strabismus, and the degree to which these influences can be explained by factors in common with refractive error. Participants were examined for the presence of latent ('phoria') and manifest ('tropia') strabismus using cover-uncover and alternate cover tests. Two phenotypes were distinguished: eso-deviation (esophoria and esotropia) and exo-deviation (exophoria and exotropia). Structural equation modeling was subsequently employed to partition the observed phenotypic variation in the twin data into specific variance components. The prevalence of eso-deviation and exo-deviation was 8.6% and 20.7%, respectively. For eso-deviation, the polychoric correlation was significantly greater in monozygotic (MZ) (r = 0.65) compared to dizygotic (DZ) twin pairs (r = 0.33), suggesting a genetic role (p = .003). There was no significant difference in polychoric correlation between MZ (r = 0.55) and DZ twin pairs (r = 0.53) for exo-deviation (p = .86), implying that genetic factors do not play a significant role in the etiology of exo-deviation. The heritability of an eso-deviation was 0.64 (95% CI 0.50-0.75). The additive genetic correlation for eso-deviation and refractive error was 0.13 and the bivariate heritability (i.e., shared variance) was less than 1%, suggesting negligible shared genetic effect. This study documents a substantial heritability of 64% for eso-deviation, yet no corresponding heritability for exo-deviation, suggesting that the genetic contribution to strabismus may be specific to eso-deviation. Future studies are now needed to identify the genes associated with eso-deviation and

  12. The line-of-sight warp of the spiral galaxy ESO 123-G23

    NARCIS (Netherlands)

    Gentile, G; Fraternali, F; Klein, U; Salucci, P

    We present 3-D modelling of the distribution and kinematics of the neutral hydrogen in the spiral galaxy ESO 123- G23. The optical appearance of this galaxy is an almost perfectly edge-on disk, while the neutral hydrogen is found to extend vertically out to about 15 kpc on either side of the

  13. The ESA/ESO/NASA Photoshop FITS Liberator 3: Have your say on new features

    Science.gov (United States)

    Nielsen, L. H.; Christensen, L. L.; Hurt, R. L.; Nielsen, K.; Johansen, T.

    2008-06-01

    The popular, free ESA/ESO/NASA Photoshop FITS Liberator image processing software (a plugin for Adobe Photoshop) is about to get simpler, faster and more user-friendly! Here we would like to solicit inputs from the community of users.

  14. X-shooter, the new wide band intermediate resolution spectrograph at the ESO Very Large Telescope

    NARCIS (Netherlands)

    Vernet, J.; Dekker, H.; D'Odorico, S.; Kaper, L.; Kjaergaard, P.; Hammer, F.; Randich, S.; Zerbi, F.; Groot, P.J.; Hjorth, J.; Guinouard, I.; Navarro, R.; Adolfse, T.; Albers, P.W.; Amans, J.-P.; Andersen, J.J.; Andersen, M.I.; Binetruy, P.; Bristow, P.; Castillo, R.; Chemla, F.; Christensen, L.; Conconi, P.; Conzelmann, R.; Dam, J.; De Caprio, V.; de Ugarte Postigo, A.; Delabre, B.; Di Marcantonio, P.; Downing, M.; Elswijk, E.; Finger, G.; Fischer, G.; Flores, H.; François, P.; Goldoni, P.; Guglielmi, L.; Haigron, R.; Hanenburg, H.; Hendriks, I.; Horrobin, M.; Horville, D.; Jessen, N.C.; Kerber, F.; Kern, L.; Kiekebusch, M.; Kleszcz, P.; Klougart, J.; Kragt, J.; Larsen, H.H.; Lizon, J.-L.; Lucuix, C.; Mainieri, V.; Manuputy, R.; Martayan, C.; Mason, E.; Mazzoleni, R.; Michaelsen, N.; Modigliani, A.; Moehler, S.; Møller, P.; Norup Sørensen, A.; Nørregaard, P.; Péroux, C.; Patat, F.; Pena, E.; Pragt, J.; Reinero, C.; Rigal, F.; Riva, M.; Roelfsema, R.; Royer, F.; Sacco, G.; Santin, P.; Schoenmaker, T.; Spano, P.; Sweers, E.; ter Horst, R.; Tintori, M.; Tromp, N.; van Dael, P.; van Vliet, H.; Venema, L.; Vidali, M.; Vinther, J.; Vola, P.; Winters, R.; Wistisen, D.; Wulterkens, G.; Zacchei, A.

    2011-01-01

    X-shooter is the first 2nd generation instrument of the ESO Very Large Telescope (VLT). It is a very efficient, single-target, intermediate-resolution spectrograph that was installed at the Cassegrain focus of UT2 in 2009. The instrument covers, in a single exposure, the spectral range from 300 to

  15. X-shooter, the new wide band intermediate resolution spectrograph at the ESO Very Large Telescope

    DEFF Research Database (Denmark)

    Vernet, J.; Dekker, H.; D'Odorico, S.

    2011-01-01

    X-shooter is the first 2nd generation instrument of the ESO Very Large Telescope (VLT). It is a very efficient, single-target, intermediate-resolution spectrograph that was installed at the Cassegrain focus of UT2 in 2009. The instrument covers, in a single exposure, the spectral range from 300 t...

  16. Extinction in the Galaxy from surface brightnesses of ESO-LV galaxies : Testing "standard" extinction maps

    NARCIS (Netherlands)

    Choloniewski, J.; Valentijn, E. A.

    A new method for the determination of the extinction in the Galaxy is proposed. The method uses surface brightnesses of external galaxies in the B and R-bands. The observational data have been taken from the ESO-LV galaxy catalog. As a first application of our model we derive the ratio of R-band to

  17. ESO 3060170: A massive fossil galaxy group with a heated gas core?

    DEFF Research Database (Denmark)

    Sun, M.; Forman, W.; Vikhlinin, A.

    2004-01-01

    10 kpc. The region between 10 and 50 kpc (the cooling radius) has the same temperature as the gas from 50 to 400 kpc, although the gas cooling time between 10 and 50 kpc (2-6 Gyr) is shorter than the Hubble time. Thus, the ESO 3060170 group does not have a group-sized cooling core. We suggest...

  18. NY-ESO-1 expression in DCIS: A new predictor of good prognosis.

    Science.gov (United States)

    Coombes, R Charles; Caballero, Otavia L; Shousha, Sami; Ghaem-Maghami, Sadaf; Woodley-Barker, Laura; Wilhelm-Benartzi, Charlotte S; Neville, A Munro

    2017-03-01

    At present, it is difficult to predict which patients with ductal carcinoma-in-situ (DCIS) will subsequently develop frank invasive breast cancer (IDC). A recent survey by our group has shown that NY-ESO-1 and MAGEA are both expressed in DCIS. This study was aimed at determining whether expression of these antigens was related to the later development of IDC. 14 of 42 (33%) of patients developed invasive breast cancer during the follow up period. Only one of those DCIS cases that relapsed was positive for NYESO-1 at diagnosis. In contrast, DCIS samples of 15 of the 28 (54%) of those patients who remained disease-free expressed NY-ESO-1. (Permutation chi square p=0.0033). We identified 42 patients with DCIS, and followed them up for more than 10 years. NY-ESO-1 and MAGEA were demonstrated by immunostaining as were CD8+ infiltrates on all sections together with the conventional markers, ER, PR, and HER2. Expression of NY-ESO-1 may predict those patients who will not subsequently develop invasive breast cancer and could therefore potentially be helpful in defining prognosis in patients with DCIS.

  19. Automatic parameter extraction for the 16,000 galaxies in the ESO/Uppsala catalogue

    NARCIS (Netherlands)

    Lauberts, A.; Valentijn, E. A.

    1983-01-01

    Under a restriction for minimum angular diameter of not less than 1 arcmin, corresponding to the 15th magnitude and allowing morphological classification of structure, 16,000 galaxies have been brought together in the single volume of the ESO/Uppsala catalog (1982). Attention is given to the

  20. European Stroke Organisation (ESO) guidelines for the management of spontaneous intracerebral hemorrhage

    DEFF Research Database (Denmark)

    Steiner, Thorsten; Al-Shahi Salman, Rustam; Beer, Ronnie

    2014-01-01

    BACKGROUND: Intracerebral hemorrhage (ICH) accounted for 9% to 27% of all strokes worldwide in the last decade, with high early case fatality and poor functional outcome. In view of recent randomized controlled trials (RCTs) of the management of ICH, the European Stroke Organisation (ESO) has...

  1. Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer.

    Science.gov (United States)

    Milne, Katy; Barnes, Rebecca O; Girardin, Adam; Mawer, Melanie A; Nesslinger, Nancy J; Ng, Alvin; Nielsen, Julie S; Sahota, Robert; Tran, Eric; Webb, John R; Wong, May Q; Wick, Darin A; Wray, Andrew; McMurtrie, Elissa; Köbel, Martin; Kalloger, Steven E; Gilks, C Blake; Watson, Peter H; Nelson, Brad H

    2008-01-01

    Tumor-infiltrating CD8+ T cells are correlated with prolonged progression-free and overall survival in epithelial ovarian cancer (EOC). A significant fraction of EOC patients mount autoantibody responses to various tumor antigens, however the relationship between autoantibodies and tumor-infiltrating T cells has not been investigated in EOC or any other human cancer. We hypothesized that autoantibody and T cell responses may be correlated in EOC and directed toward the same antigens. We obtained matched serum and tumor tissue from 35 patients with high-grade serous ovarian cancer. Serum samples were assessed by ELISA for autoantibodies to the common tumor antigen NY-ESO-1. Tumor tissue was examined by immunohistochemistry for expression of NY-ESO-1, various T cell markers (CD3, CD4, CD8, CD25, FoxP3, TIA-1 and Granzyme B) and other immunological markers (CD20, MHC class I and MHC class II). Lymphocytic infiltrates varied widely among tumors and included cells positive for CD3, CD8, TIA-1, CD25, FoxP3 and CD4. Twenty-six percent (9/35) of patients demonstrated serum IgG autoantibodies to NY-ESO-1, which were positively correlated with expression of NY-ESO-1 antigen by tumor cells (r = 0.57, p = 0.0004). Autoantibodies to NY-ESO-1 were associated with increased tumor-infiltrating CD8+, CD4+ and FoxP3+ cells. In an individual HLA-A2+ patient with autoantibodies to NY-ESO-1, CD8+ T cells isolated from solid tumor and ascites were reactive to NY-ESO-1 by IFN-gamma ELISPOT and MHC class I pentamer staining. We demonstrate that tumor-specific autoantibodies and tumor-infiltrating T cells are correlated in human cancer and can be directed against the same target antigen. This implies that autoantibodies may collaborate with tumor-infiltrating T cells to influence clinical outcomes in EOC. Furthermore, serological screening methods may prove useful for identifying clinically relevant T cell antigens for immunotherapy.

  2. Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer.

    Directory of Open Access Journals (Sweden)

    Katy Milne

    Full Text Available BACKGROUND: Tumor-infiltrating CD8+ T cells are correlated with prolonged progression-free and overall survival in epithelial ovarian cancer (EOC. A significant fraction of EOC patients mount autoantibody responses to various tumor antigens, however the relationship between autoantibodies and tumor-infiltrating T cells has not been investigated in EOC or any other human cancer. We hypothesized that autoantibody and T cell responses may be correlated in EOC and directed toward the same antigens. METHODOLOGY AND PRINCIPAL FINDINGS: We obtained matched serum and tumor tissue from 35 patients with high-grade serous ovarian cancer. Serum samples were assessed by ELISA for autoantibodies to the common tumor antigen NY-ESO-1. Tumor tissue was examined by immunohistochemistry for expression of NY-ESO-1, various T cell markers (CD3, CD4, CD8, CD25, FoxP3, TIA-1 and Granzyme B and other immunological markers (CD20, MHC class I and MHC class II. Lymphocytic infiltrates varied widely among tumors and included cells positive for CD3, CD8, TIA-1, CD25, FoxP3 and CD4. Twenty-six percent (9/35 of patients demonstrated serum IgG autoantibodies to NY-ESO-1, which were positively correlated with expression of NY-ESO-1 antigen by tumor cells (r = 0.57, p = 0.0004. Autoantibodies to NY-ESO-1 were associated with increased tumor-infiltrating CD8+, CD4+ and FoxP3+ cells. In an individual HLA-A2+ patient with autoantibodies to NY-ESO-1, CD8+ T cells isolated from solid tumor and ascites were reactive to NY-ESO-1 by IFN-gamma ELISPOT and MHC class I pentamer staining. CONCLUSION AND SIGNIFICANCE: We demonstrate that tumor-specific autoantibodies and tumor-infiltrating T cells are correlated in human cancer and can be directed against the same target antigen. This implies that autoantibodies may collaborate with tumor-infiltrating T cells to influence clinical outcomes in EOC. Furthermore, serological screening methods may prove useful for identifying clinically relevant

  3. ESO Receives Computerworld Honors Program 21st Century Achievement Award in Science Category

    Science.gov (United States)

    2005-06-01

    In a ceremony held in Washington, D.C. (USA) on June 6, 2005, ESO, the European Organisation for Astronomical Research in the southern Hemisphere, received the coveted 21st Century Achievement Award from the Computerworld Honors Program for its visionary use of information technology in the Science category. Sybase, a main database server vendor and member of the Chairmen's Committee, nominated ESO's Data Flow System in recognition of its contributions to the global information technology revolution and its positive impact on society. The citations reads: "ESO has revolutionized the operations of ground-based astronomical observatories with a new end-to-end data flow system, designed to improve the transmission and management of astronomical observations and data over transcontinental distances." This year's awards, in 10 categories, were presented at a gala event at the National Building Museum, attended by over 250 guests, including leaders of the information technology industry, former award recipients, judges, scholars, and diplomats representing many of the 54 countries from which the 17-year-old program's laureates have come. "The Computerworld Honors Program 21st Century Achievement Awards are presented to companies from around the world whose visionary use of information technology promotes positive social, economic and educational change," said Bob Carrigan, president and CEO of Computerworld and chairman of the Chairmen's Committee of the Computerworld Honors Program. "The recipients of these awards are the true heroes of the information age and have been appropriately recognized by the leading IT industry chairmen as true revolutionaries in their fields." ESO PR Photo 18/05 ESO PR Photo 18/05 ESO Receives the Award in the Science Category [Preview - JPEG: 400 x 496 pix - 53k] [Normal - JPEG: 800 x 992 pix - 470k] [Full Res - JPEG: 1250 x 1550 pix - 1.1M] Caption: ESO PR Photo 18/05: Receiving the Computerworld 21st Century Achievement Award for Science

  4. A potential role for immunotherapy in thyroid cancer by enhancing NY-ESO-1 cancer antigen expression.

    Science.gov (United States)

    Gunda, Viswanath; Frederick, Dennie T; Bernasconi, Maria J; Wargo, Jennifer A; Parangi, Sareh

    2014-08-01

    NY-ESO-1 is one of the most immunogenic members of the cancer/testis antigen family and its levels can be increased after exposure to demethylating and deacetylating agents. This cytoplasmic antigen can serve as a potent target for cancer immunotherapy and yet has not been well studied in differentiated thyroid cancer cells. We studied the baseline expression of NY-ESO-1 messenger RNA and protein before and after exposure to 5-aza-2'-deoxycytidine (DAC) (72 hours) in a panel of thyroid cancer cell lines using quantitative polymerase chain reaction and Western blot. HLA-A2+, NY-ESO-1+ thyroid cell lines were then co-cultured with peripheral blood lymphocytes transduced with NY-ESO-1 specific T-cell receptor (TCR) and assayed for interferon-gamma and Granzyme-B release in the medium. SCID mice injected orthotopically with BCPAP cells were treated with DAC to evaluate for NY-ESO-1 gene expression in vivo. None of the thyroid cancer cell lines showed baseline expression of NY-ESO-1. Three cell lines, BCPAP, TPC-1, and 8505c, showed an increase in NY-ESO-1 gene expression with DAC treatment and were found to be HLA-A2 positive. DAC-treated target BCPAP and TPC-1 tumor cells with up-regulated NY-ESO-1 levels were able to mount an appropriate interferon-gamma and Granzyme-B response upon co-culture with the NY-ESO-1-TCR-transduced peripheral blood lymphocytes. In vivo DAC treatment was able to increase NY-ESO-1 expression in an orthotopic mouse model with BCPAP cells. Our data suggest that many differentiated thyroid cancer cells can be pressed to express immune antigens, which can then be utilized in TCR-based immunotherapeutic interventions.

  5. ESO unveils an amazing, interactive, 360-degree panoramic view of the entire night sky

    Science.gov (United States)

    2009-09-01

    The first of three images of ESO's GigaGalaxy Zoom project - a new magnificent 800-million-pixel panorama of the entire sky as seen from ESO's observing sites in Chile - has just been released online. The project allows stargazers to explore and experience the Universe as it is seen with the unaided eye from the darkest and best viewing locations in the world. This 360-degree panoramic image, covering the entire celestial sphere, reveals the cosmic landscape that surrounds our tiny blue planet. This gorgeous starscape serves as the first of three extremely high-resolution images featured in the GigaGalaxy Zoom project, launched by ESO within the framework of the International Year of Astronomy 2009 (IYA2009). GigaGalaxy Zoom features a web tool that allows users to take a breathtaking dive into our Milky Way. With this tool users can learn more about many different and exciting objects in the image, such as multicoloured nebulae and exploding stars, just by clicking on them. In this way, the project seeks to link the sky we can all see with the deep, "hidden" cosmos that astronomers study on a daily basis. The wonderful quality of the images is a testament to the splendour of the night sky at ESO's sites in Chile, which are the most productive astronomical observatories in the world. The plane of our Milky Way Galaxy, which we see edge-on from our perspective on Earth, cuts a luminous swath across the image. The projection used in GigaGalaxy Zoom place the viewer in front of our Galaxy with the Galactic Plane running horizontally through the image - almost as if we were looking at the Milky Way from the outside. From this vantage point, the general components of our spiral galaxy come clearly into view, including its disc, marbled with both dark and glowing nebulae, which harbours bright, young stars, as well as the Galaxy's central bulge and its satellite galaxies. The painstaking production of this image came about as a collaboration between ESO, the renowned

  6. Polymeric structure and host Toll-like receptor 4 dictate immunogenicity of NY-ESO-1 antigen in vivo.

    Science.gov (United States)

    Liu, Yanan; Tian, Xiaoli; Leitner, Wolfgang W; Aldridge, Michael E; Zheng, Junying; Yu, Zhiya; Restifo, Nicholas P; Weiss, Richard; Scheiblhofer, Sandra; Xie, Chong; Sun, Ren; Cheng, Genhong; Zeng, Gang

    2011-10-28

    In search of intrinsic factors that contribute to the distinctively strong immunogenicity of a non-mutated cancer/testis antigen, we found that NY-ESO-1 forms polymeric structures through disulfide bonds. NY-ESO-1 binding to immature dendritic cells was dependent on its polymeric structure and involved Toll-like receptor-4 (TLR4) on the surface of immature dendritic cells in mouse and human. Gene gun-delivered plasmid encoding the wild-type NY-ESO-1 readily induced T cell-dependent antibody (Ab) responses in wild-type C57BL/10 mice but not TLR4-knock-out C57BL/10ScNJ mice. Disrupting polymeric structures of NY-ESO-1 by cysteine-to-serine (Cys-to-Ser) substitutions lead to diminished immunogenicity and altered TLR4-dependence in the induced Ab response. To demonstrate its adjuvant effect, NY-ESO-1 was fused with a major mugwort pollen allergen Art v 1 and a tumor-associated antigen, carbonic anhydrase 9. Plasmid DNA vaccines encoding the fusion genes generated robust immune responses against otherwise non-immunogenic targets in mice. Polymeric structure and TLR4 may play important roles in rendering NY-ESO-1 immunogenic and thus serve as a potent molecular adjuvant. NY-ESO-1 thus represents the first example of a cancer/testis antigen that is a also damage-associated molecular pattern.

  7. Polymeric Structure and Host Toll-like Receptor 4 Dictate Immunogenicity of NY-ESO-1 Antigen in Vivo*

    Science.gov (United States)

    Liu, Yanan; Tian, Xiaoli; Leitner, Wolfgang W.; Aldridge, Michael E.; Zheng, Junying; Yu, Zhiya; Restifo, Nicholas P.; Weiss, Richard; Scheiblhofer, Sandra; Xie, Chong; Sun, Ren; Cheng, Genhong; Zeng, Gang

    2011-01-01

    In search of intrinsic factors that contribute to the distinctively strong immunogenicity of a non-mutated cancer/testis antigen, we found that NY-ESO-1 forms polymeric structures through disulfide bonds. NY-ESO-1 binding to immature dendritic cells was dependent on its polymeric structure and involved Toll-like receptor-4 (TLR4) on the surface of immature dendritic cells in mouse and human. Gene gun-delivered plasmid encoding the wild-type NY-ESO-1 readily induced T cell-dependent antibody (Ab) responses in wild-type C57BL/10 mice but not TLR4-knock-out C57BL/10ScNJ mice. Disrupting polymeric structures of NY-ESO-1 by cysteine-to-serine (Cys-to-Ser) substitutions lead to diminished immunogenicity and altered TLR4-dependence in the induced Ab response. To demonstrate its adjuvant effect, NY-ESO-1 was fused with a major mugwort pollen allergen Art v 1 and a tumor-associated antigen, carbonic anhydrase 9. Plasmid DNA vaccines encoding the fusion genes generated robust immune responses against otherwise non-immunogenic targets in mice. Polymeric structure and TLR4 may play important roles in rendering NY-ESO-1 immunogenic and thus serve as a potent molecular adjuvant. NY-ESO-1 thus represents the first example of a cancer/testis antigen that is a also damage-associated molecular pattern. PMID:21900253

  8. ESO takes the public on an astronomical journey "Around the World in 80 Telescopes"

    Science.gov (United States)

    2009-03-01

    A live 24-hour free public video webcast, "Around the World in 80 Telescopes", will take place from 3 April 09:00 UT/GMT to 4 April 09:00 UT/GMT, chasing day and night around the globe to let viewers "visit" some of the most advanced astronomical telescopes on and off the planet. The webcast, organised by ESO for the International Year of Astronomy 2009 (IYA2009), is the first time that so many large observatories have been linked together for a public event. ESO PR Photo 13a/09 Map of Participating Observatories ESO PR Photo 13b/09 100 Hours of Astronomy logo Viewers will see new images of the cosmos, find out what observatories in their home countries or on the other side of the planet are discovering, send in questions and messages, and discover what astronomers are doing right now. Participating telescopes include those at observatories in Chile such as ESO's Very Large Telescope and La Silla, the Hawaii-based telescopes Gemini North and Keck, the Anglo-Australian Telescope, telescopes in the Canary Islands, the Southern African Large Telescope, space-based telescopes such as the NASA/ESA Hubble Space Telescope, ESA XMM-Newton and Integral, and many more. "Around the World in 80 Telescopes" will take viewers to every continent, including Antarctica! The webcast production will be hosted at ESO's headquarters near Munich, Germany, with live internet streaming by Ustream.tv. Anyone with a web browser supporting Adobe Flash will be able to follow the show, free of charge, from the website www.100hoursofastronomy.org and be a part of the project by sending messages and questions. The video player can be freely embedded on other websites. TV stations, web portals and science centres can also use the high quality feed. Representatives of the media who wish to report from the "front-line" and interview the team should get in touch. "Around the World in 80 Telescopes" is a major component of the 100 Hours of Astronomy (100HA), a Cornerstone project of the International

  9. Effector memory and central memory NY-ESO-1-specific re-directed T cells for treatment of multiple myeloma.

    Science.gov (United States)

    Schuberth, P C; Jakka, G; Jensen, S M; Wadle, A; Gautschi, F; Haley, D; Haile, S; Mischo, A; Held, G; Thiel, M; Tinguely, M; Bifulco, C B; Fox, B A; Renner, C; Petrausch, U

    2013-04-01

    The cancer-testis antigen NY-ESO-1 is a potential target antigen for immune therapy expressed in a subset of patients with multiple myeloma. We generated chimeric antigen receptors (CARs) recognizing the immunodominant NY-ESO-1 peptide 157-165 in the context of HLA-A*02:01 to re-direct autologous CD8(+) T cells towards NY-ESO-1(+) myeloma cells. These re-directed T cells specifically lysed NY-ESO-1(157-165)/HLA-A*02:01-positive cells and secreted IFNγ. A total of 40% of CCR7(-) re-directed T cells had an effector memory phenotype and 5% a central memory phenotype. Based on CCR7 cell sorting, effector and memory CAR-positive T cells were separated and CCR7(+) memory cells demonstrated after antigen-specific re-stimulation downregulation of CCR7 as sign of differentiation towards effector cells accompanied by an increased secretion of memory signature cytokines such as IL-2. To evaluate NY-ESO-1 as potential target antigen, we screened 78 bone marrow biopsies of multiple myeloma patients where NY-ESO-1 protein was found to be expressed by immunohistochemistry in 9.7% of samples. Adoptively transferred NY-ESO-1-specific re-directed T cells protected mice against challenge with endogenously NY-ESO-1-positive myeloma cells in a xenograft model. In conclusion, re-directed effector- and central memory T cells specifically recognized NY-ESO-1(157-165)/ HLA-A*02:01-positive cells resulting in antigen-specific functionality in vitro and in vivo.

  10. Enhancing whole-tumor cell vaccination by engaging innate immune system through NY-ESO-1/dendritic cell interactions.

    Science.gov (United States)

    Xu, Le; Zheng, Junying; Nguyen, David H; Luong, Quang T; Zeng, Gang

    2013-10-01

    NY-ESO-1 is a cancer/germline antigen (Ag) with distinctively strong immunogenicity. We have previously demonstrated that NY-ESO-1 serves as an endogenous adjuvant by engaging dendritic cell (DC)-surface receptors of calreticulin (CRT) and toll-like receptor (TLR) 4. In the present study, NY-ESO-1 was investigated for its immunomodulatory roles as a molecular adjuvant in whole-tumor cell vaccines using the Renca kidney cancer model. Renca cells were genetically engineered to express NY-ESO-1 on the cell surface to enhance direct interactions with DC. The effect of ectopic cell-surface expression of NY-ESO-1 was investigated on tumor immunogenicity, DC activation, cytotoxic T lymphocytes against model tumor-associated Ags, and the effectiveness of the modified tumor cells as a therapeutic whole-cell vaccine. Cell-surface expression of NY-ESO-1 was able to reduce the tumor growth of Renca cells in BALB/c mice, although the modification did not alter cell proliferation rate in vitro. Directly engaging the innate immune system through NY-ESO-1 facilitated the interaction of tumor cells with DC, leading to enhanced DC activation and subsequent tumor-specific T-cell priming. When used as a therapeutic whole-cell vaccine, Renca cells with NY-ESO-1 on the surface mediated stronger inhibitory effects on tumor growth and metastasis compared with parental Renca or Renca cells expressing a control protein GFP on the surface. Augmented antitumor efficacy correlated with increased CD8 T-cell infiltration into tumors and decreased myeloid-derived suppressor cells and regulatory T cells in the spleen. As a cancer/germline Ag and as an immunomodulatory adjuvant through engaging innate immune receptors, NY-ESO-1 offers a unique opportunity for improved whole-tumor cell vaccinations upon the classic GM-CSF-engineered cell vaccines.

  11. NY-ESO-1 is a ubiquitous immunotherapeutic target antigen for patients with myxoid/round cell liposarcoma.

    Science.gov (United States)

    Pollack, Seth M; Jungbluth, Achim A; Hoch, Benjamin L; Farrar, Erik A; Bleakley, Marie; Schneider, David J; Loggers, Elizabeth T; Rodler, Eve; Eary, Janet F; Conrad, Ernest U; Jones, Robin L; Yee, Cassian

    2012-09-15

    Myxoid/round cell liposarcoma (MRCL) is the second most common liposarcoma subtype, accounting for >33% of liposarcomas and approximately 10% of all soft tissue sarcomas. Although MRCL is a chemosensitive subtype, patients with metastatic disease have a poor outcome. NY-ESO-1 is a cancer-testis antigen (also known as cancer germ cell antigen) that has been successfully targeted in vaccine trials and in adoptive T-cell therapy trials for the treatment of several solid tumors. The authors investigated the feasibility of targeting NY-ESO-1 in patients with MRCL by evaluating the prevalence of NY-ESO-1 expression in tumors using immunohistochemistry and quantitative reverse transcriptase-polymerase chain reaction analysis. NY-ESO-1-specific tumor recognition by NY-ESO-1-specific T-cells also was analyzed using a chromium release assay. A search of the University of Washington Sarcoma Tissue Bank identified paraffin-embedded tumor samples from 25 patients with MRCL. NY-ESO-1 expression was observed in every MRCL tumor assessed (100%); in 18 tumors (72%), staining was homogenous. In all but 2 tumors, staining was sufficiently robust (2+) that such patients would be eligible for clinical trials of NY-ESO-1-directed therapy. By using NY-ESO-1 specific, CD8-positive T-cells, the in vitro sensitivity of myxoid liposarcoma cell lines to antigen-specific lysis was demonstrated. The current results establish NY-ESO-1 as an important target antigen for the treatment of patients with MRCL. Copyright © 2012 American Cancer Society.

  12. MAGE-A3/4 and NY-ESO-1 antigens expression in metastatic esophageal squamous cell carcinoma.

    Science.gov (United States)

    Bujas, T; Marusic, Z; Peric Balja, M; Mijic, A; Kruslin, B; Tomas, D

    2011-03-21

    In the present study we analyzed immunohistochemical expression of MAGE-A 3/4 and NY-ESO-1 in 55 samples of esophageal squamous cell carcinomas (ESCC) and their respective lymph node metastases. To our knowledge this is the first study to assess and compare the expression of these antigens in ESCC lymph node metastases. Fifty (90.9%) primary ESCC were positive for MAGE-A 3/4 and 53 (96.6%) were positive for NY-ESO-1. MAGE-A 3/4 was expressed in all lymph node metastases and the intensity of expression was high in a majority of cases. NY-ESO-1 was negative in 2 (7.1%) lymph nodes metastases, while the reaction was predominantly moderate in the positive group. In primary tumors MAGE-A 3/4 showed a significantly higher intensity of expression compared to NY-ESO-1 (P=0.047), while in lymph node metastases the intensity of expression was not significantly different (P=0.387). Primary tumors with and without lymph node metastases showed no significant differences in MAGE-A 3/4 (P=0.672) and NY-ESO-1 (P=0.444) expression. Intensity of MAGE-A 3/4 (P=0.461) and NY-ESO-1 (P=0.414) expression in primary tumors was not significantly different compared to the expression in their respective lymph nodes metastases. Expression of MAGE-A 3/4 in primary tumors showed significant positive correlation with primary tumor expression of NY-ESO-1 (P=0.021) but no significant correlation with the expression of MAGE-A 3/4 in lymph node metastases (P=0.056). Expression of NY-ESO-1 in primary tumors showed significant positive correlation with the expression of NY-ESO-1 in lymph node metastases (P=0.001) and significant negative correlation with patients’ age (P<0.001). Expression of MAGE-A 3/4 and NY-ESO-1 in primary tumors and lymph node metastases showed no significant correlation with prognostic parameters such as tumor grade and TNM stage (P>0.05). We have shown different levels of MAGE-A 3/4 and NY-ESO-1 expression in almost all specimens of primary tumor and lymph node metastases

  13. Informática Segundo ESO. Bloque 2: Internet y seguridad informática

    OpenAIRE

    Badenes Donoso, Vicente

    2015-01-01

    Treball Final de Màster Universitari en Professor/a d'Educació Secundària Obligatòria i Batxillerat, Formació Professional i Ensenyaments d'Idiomes. Codi: SAP129. Curs acadèmic: 2014-2015 El presente Trabajo Final de Máster se enmarca dentro de la modalidad 3 de TFM, Planificación y/o Programación curricular de la asignatura de Informática para los alumnos/as de 2º de ESO. Aunque en este curso 2015/16, en los cursos de 2º y 4º de ESO, las enseñanzas mínimas de la Educación Secundaria Ob...

  14. ESO 103-G35 - A new Seyfert galaxy and possible X-ray source

    Science.gov (United States)

    Phillips, M. M.; Feldman, F. R.; Marshall, F. E.; Wamsteker, W.

    1979-01-01

    By means of an objective prism plate, two emission-line galaxies have been identified within the 0.7-sq deg HEAO-A2 error box for the X-ray source H1834-653. Optical spectrophotometric observations are reported for both objects as well as the galaxy NGC 6684, which also lies near the position of H1834-653. These data show that one of the emission-line galaxies, ESO 103-G35, is a Seyfert galaxy with a high-excitation forbidden-line spectrum and weak broad emission wings at H-alpha. Further measurements of this galaxy reveal an infrared excess at wavelengths longer than 2.2 microns. The H-alpha luminosity of ESO 103-G35 is consistent with the X-ray luminosity estimated from the HEAO-A2 data, thus strengthening the likelihood of association of this galaxy with the X-ray emission.

  15. On the Availability of ESO Data Papers on arXiv/astro-ph

    Science.gov (United States)

    Grothkopf, U.; Bordelon, D.; Meakins, S.; Emsellem, E.

    2017-12-01

    Using the ESO Telescope Bibliography database telbib, we have investigated the percentage of ESO data papers that were submitted to the arXiv/astro-ph e-print server and that are therefore free to read. Our study revealed an availability of up to 96 % of telbib papers on arXiv over the years 2010 to 2017. We also compared the citation counts of arXiv vs. non-arXiv papers and found that on average, papers submitted to arXiv are cited 2.8 times more often than those not on arXiv. While simulations suggest that these findings are statistically significant, we cannot yet draw firm conclusions as to the main cause of these differences.

  16. Britain Approaches ESO about Installation of Major New Telescope at Paranal

    Science.gov (United States)

    2000-02-01

    The Executive Board of the UK Visible and Infrared Survey Telescope (VISTA) project announced today [1] that it is aiming at the installation of a new and powerful astronomical telescope at the ESO Paranal Observatory (Chile). This 4-metre telescope is a specialised wide-angle facility equipped with powerful cameras and efficient detectors that will enable it to obtain deep images of large sky areas in short time. These survey observations will be made in several wavebands in the optical and, in particular, the near-infrared region of the electromagnetic spectrum. VISTA will become the largest and most effective telescope of its type when it enters into operation in 2004. It is a project of a consortium of 18 UK universities [2]. Construction is expected to start in spring 2000. Funding of the project was announced in May 1999, as one of the first allocations from the "Joint Infrastructure Fund (JIF)", an initiative of the UK Government's Department of Trade and Industry, the Wellcome Trust, and the Higher Education Funding Council for England. ESO's Director General, Dr. Catherine Cesarsky , is very pleased with this decision. She received a mandate from the ESO Council in December 1999 to negotiate a contract with the UK Particle Physics and Astronomy Research Council (PPARC) , acting on behalf of the VISTA Executive Board, for the installation of VISTA at Paranal and now looks forward to settle the associated legal and operational details with her British counterparts at good pace. "The installation of VISTA at Paranal will be of great benefit to all European astronomers", she says. "The placement of a survey telescope of this size next to ESO's VLT, the world's largest optical telescope, opens a plethora of exciting opportunities for joint research projects. Deep observations with VISTA, especially in infrared wavebands, will provide a most valuable, first census of large regions of space. This will most certainly lead to the discoveries of many new and

  17. A deep X-ray spectroscopic survey of the ESO imaging survey fields

    DEFF Research Database (Denmark)

    Nørgaard-Nielsen, Hans Ulrik; Jørgensen, H.E.; Hansen, Lene

    1998-01-01

    V, we propose to perform a spectroscopic survey with SODART and push these telescopes to the limit at the high energies. By selecting the four ESO Imaging Survey fields EIS teach 6 deg(2)) we will take full advantage of the large, systematic effort ESO is putting into the optical survey of these fields......The deepest ROSAT surveys have shown, that, in the energy range 0.5-2.0 keV, QSO's can account for similar to 30 per cent of the Diffuse X-ray Background (DXRB), and Narrow Emission Line Galaxies (NELG) and clusters of galaxies for about 10 per cent each. But, by assuming characteristic spectral....... This spectroscopic X-ray survey will provide a large, statistically complete, sample of sources detected at high energies, more than an order of magnitude fainter than obtained by previous missions. The study of these sources will significantly improve our understanding not only of the origin of DXRB, but also...

  18. The Gaia-ESO Survey: detailed abundances for thousands of FGK-type stars

    Science.gov (United States)

    Smiljanic, R.

    2014-10-01

    The Gaia-ESO Survey is using FLAMES at the VLT to observe more than 10^{5} stars. Giraffe medium-resolution spectra is being obtained for ˜ 10^{5} stars and high-resolution UVES spectra is being obtained for ˜ 5000 stars. Here I present a short summary of the Survey with emphasis on the sample of FGK-type stars being observed with UVES.

  19. Immunohistochemical assessment of NY-ESO-1 expression in esophageal adenocarcinoma resection specimens.

    Science.gov (United States)

    Hayes, Stephen J; Hng, Keng Ngee; Clark, Peter; Thistlethwaite, Fiona; Hawkins, Robert E; Ang, Yeng

    2014-04-14

    To assess NY-ESO-1 expression in a cohort of esophageal adenocarcinomas. A retrospective search of our tissue archive for esophageal resection specimens containing esophageal adenocarcinoma was performed, for cases which had previously been reported for diagnostic purposes, using the systematised nomenclature of human and veterinary medicine coding system. Original haematoxylin and eosin stained sections were reviewed, using light microscopy, to confirm classification and tumour differentiation. A total of 27 adenocarcinoma resection specimens were then assessed using immunohistochemistry for NY-ESO-1 expression: 4 well differentiated, 14 moderately differentiated, 4 moderate-poorly differentiated, and 5 poorly differentiated. Four out of a total of 27 cases of esophageal adenocarcinoma examined (15%) displayed diffuse cytoplasmic and nuclear expression for NY-ESO-1. They displayed a heterogeneous and mosaic-type pattern of diffuse staining. Diffuse cytoplasmic staining was not identified in any of these structures: stroma, normal squamous epithelium, normal submucosal gland and duct, Barrett's esophagus (goblet cell), Barrett's esophagus (non-goblet cell) and high grade glandular dysplasia. All adenocarcinomas showed an unexpected dot-type pattern of staining at nuclear, paranuclear and cytoplasmic locations. Similar dot-type staining, with varying frequency and size of dots, was observed on examination of Barrett's metaplasia, esophageal submucosal gland acini and the large bowel negative control, predominantly at the crypt base. Furthermore, a prominent pattern of apical (luminal) cytoplasmic dot-type staining was observed in some cases of Barrett's metaplasia and also adenocarcinoma. A further morphological finding of interest was noted on examination of haematoxylin and eosin stained sections, as aggregates of lymphocytes were consistently noted to surround submucosal glands. We have demonstrated for the first time NY-ESO-1 expression by esophageal

  20. Strong lens search in the ESO public Survey KiDS

    NARCIS (Netherlands)

    Napolitano, N. R.; Covone, G.; Roy, N.; Tortora, C.; Barbera, F. La; Radovich, M.; Getman, F.; Capaccioli, M.; Colonna, A.; Paolillo, M.; Kleijn, G. A. Verdoes; Koopmans, L. V. E.; Valentijn, E; Napolitano, Nicola R.; Longo, Giuseppe; Marconi, Marcella; Paolillo, Maurizio; Lodice, Enrichetta

    2015-01-01

    We have started a systematic search for strong lens candidates in the ESO public survey KiDS based on the visual inspection of massive galaxies in the redshift range 0. 1 < z < 0. 5. As a pilot program we have inspected 100 deg2, which overlap with SDSS and where there are known lenses to use as a

  1. TCS and peripheral robotization and upgrade on the ESO 1-meter telescope at La Silla Observatory

    Science.gov (United States)

    Ropert, S.; Suc, V.; Jordán, A.; Tala, M.; Liedtke, P.; Royo, S.

    2016-07-01

    In this work we describe the robotization and upgrade of the ESO 1m telescope located at La Silla Observatory. The ESO 1m telescope was the first telescope installed in La Silla, in 1966. It now hosts as a main instrument the FIber Dual EchellE Optical Spectrograph (FIDEOS), a high resolution spectrograph designed for precise Radial Velocity (RV) measurements on bright stars. In order to meet this project's requirements, the Telescope Control System (TCS) and some of its mechanical peripherals needed to be upgraded. The TCS was also upgraded into a modern and robust software running on a group of single board computers interacting together as a network with the CoolObs TCS developed by ObsTech. One of the particularities of the CoolObs TCS is that it allows to fuse the input signals of 2 encoders per axis in order to achieve high precision and resolution of the tracking with moderate cost encoders. One encoder is installed on axis at the telescope and the other on axis at the motor. The TCS was also integrated with the FIDEOS instrument system so that all the system can be controlled through the same remote user interface. Our modern TCS unit allows the user to run observations remotely through a secured internet web interface, minimizing the need of an on-site observer and opening a new age in robotic astronomy for the ESO 1m telescope.

  2. NY-ESO-1 Protein Cancer Vaccine With Poly-ICLC and OK-432: Rapid and Strong Induction of NY-ESO-1-specific Immune Responses by Poly-ICLC.

    Science.gov (United States)

    Takeoka, Tomohira; Nagase, Hirotsugu; Kurose, Koji; Ohue, Yoshihiro; Yamasaki, Makoto; Takiguchi, Shuji; Sato, Eiichi; Isobe, Midori; Kanazawa, Takayuki; Matsumoto, Mitsunobu; Iwahori, Kota; Kawashima, Atsunari; Morimoto-Okazawa, Akiko; Nishikawa, Hiroyoshi; Oka, Mikio; Pan, Linda; Venhaus, Ralph; Nakayama, Eiichi; Mori, Masaki; Doki, Yuichiro; Wada, Hisashi

    2017-03-23

    We conducted a clinical trial of a cancer vaccine using NY-ESO-1 protein with polyinosinic-polycytidylic acid-poly-L-lysine carboxymethylcellulose (poly-ICLC) and/or OK-432 against solid tumors. A total of 15 patients were sequentially enrolled in 4 cohorts. Patients in cohort 1 received NY-ESO-1 protein; cohort 2a received NY-ESO-1 protein+OK-432; cohort 2b received NY-ESO-1 protein+poly-ICLC; cohort 3 received NY-ESO-1 protein+OK-432+poly-ICLC with Montanide ISA-51. The endpoints of this trial were safety, NY-ESO-1 immune responses, and clinical response. Vaccine-related adverse events observed were fever and injection-site reaction (grade 1). Two patients showed stable disease after vaccination. NY-ESO-1 antibodies were observed in 4 patients at the baseline (sero-positive) and augmented in all patients after vaccination. Eleven patients showed a conversion of negative antibody responses at baseline to positive after vaccination (seroconversion). The seroconversions were observed in all 11 sero-negative patients by the fourth immunization; in particular, it was observed by the second immunization in patients with poly-ICLC, and these induced antibody responses were stronger than those in patients immunized without poly-ICLC. The number of NY-ESO-1-specific interferon (IFN)γ-producing T cells was increased in patients immunized with poly-ICLC and/or OK-432, and furthermore, the increase of IFNγ-producing CD8 T cells in patients immunized with poly-ICLC was significantly higher than that in patients without poly-ICLC. Nonspecific activations of T-cell or antigen presenting cells were not observed. Our present study showed that poly-ICLC is a promising adjuvant for cancer vaccines.

  3. MOSE: A Demonstrator for an Automatic Operational System for the Optical Turbulence Forecast for ESO Sites

    Science.gov (United States)

    Masciadri, Elena; Lascaux, F.; Turchi, A.; Fini, L.

    2017-09-01

    "Most of the observations performed with new-generation ground-based telescopes are employing the Service Mode. To optimize the flexible-scheduling of scientific programs and instruments, the optical turbulence (OT) forecast is a must, particularly when observations are supported by adaptive optics (AO) and Interferometry. Reliable OT forecast are crucial to optimize the usage of AO and interferometric facilities which is not possible when using only optical measurements. Numerical techniques are the best placed to achieve such a goal. The MOSE project (MOdeling ESO Sites), co-funded by ESO, aimed at proving the feasibility of the forecast of (1) all the classical atmospheric parameters (such as temperature, wind speed and direction, relative humidity) and (2) the optical turbulence i.e. the CN 2 profiles and all the main integrated astro-climatic parameters derived from the CN 2 (the seeing, the isoplanatic angle, the wavefront coherence time) above the two ESO sites of Cerro Paranal and Cerro Armazones. The proposed technique is based on the use of a non-hydrostatic atmospheric meso-scale model and a dedicated code for the optical turbulence. The final goal of the project aimed at implementing an automatic system for the operational forecasts of the aforementioned parameters to support the astronomical observations above the two sites. MOSE Phase A and B have been completed and a set of dedicated papers have been published on the topic. Model performances have been extensively quantified with several dedicated figures of merit and we proved that our tool is able to provide reliable forecasts of optical turbulence and atmospheric parameters with very satisfactory score of success. This should guarantee us to make a step ahead in the framework of the Service Mode of new generation telescopes. A conceptual design as well as an operational plan of the automatic system has been submitted to ESO as integral part of the feasibility study. We completed a negotiation with

  4. Resiquimod as an Immunologic Adjuvant for NY-ESO-1 Protein Vaccination in Patients with High Risk Melanoma

    Science.gov (United States)

    Gnjatic, Sacha; Cruz, Crystal M.; Vengco, Isabelita; Hasan, Farah; Spadaccia, Meredith; Darvishian, Farbod; Chiriboga, Luis; Holman, Rose Marie; Escalon, Juliet; Muren, Caroline; Escano, Crystal; Yepes, Ethel; Sharpe, Dunbar; Vasilakos, John P.; Rolnitzsky, Linda; Goldberg, Judith; Mandeli, John; Adams, Sylvia; Jungbluth, Achim; Pan, Linda; Venhaus, Ralph; Ott, Patrick A.; Bhardwaj, Nina

    2015-01-01

    The TLR7/8 agonist, Resiquimod has been used as an immune adjuvant in cancer vaccines. We evaluated the safety and immunogenicity of the cancer testis antigen NY-ESO-1 given in combination with Montanide with or without Resiquimod in high-risk melanoma patients. In Part I of the study, patients received 100ug full length NY-ESO-1 protein emulsified in 1.25mL Montanide (day 1) followed by topical application of 1000mg of 0.2% Resiquimod gel on days 1 and 3 (Cohort 1) versus days 1, 3, and 5 (Cohort 2) of a 21 day cycle. In Part II, patients were randomized to receive 100ug NY-ESO-1 protein plus Montanide (day 1) followed by topical application of placebo gel (Arm-A; N=8) or 1000mg of 0.2% Resiquimod gel (Arm-B; N=12) using the dosing regimen established in Part I. The vaccine regimens were generally well-tolerated. NY-ESO-1-specific humoral responses were induced or boosted in all patients, many of whom had high titer antibodies. In Part II, 16 of 20 patients in both arms had NY-ESO-1-specific CD4+ T-cell responses. CD8+ T-cell responses were only seen in 3 of 12 patients in Arm B. Patients with TLR7 SNP rs179008 had a greater likelihood of developing NY-ESO-1-specific CD8+ responses. In conclusion, NY-ESO-1 protein in combination with Montanide with or without topical Resiquimod is safe and induces both antibody and CD4+ T-cell responses in the majority of patients; the small proportion of CD8+ T-cell responses suggests that the addition of topical Resiquimod to Montanide is not sufficient to induce consistent NY-ESO-1-specific CD8+ T-cell responses. PMID:25633712

  5. The OmegaCAM 16K x 16K CCD detector system for the ESO VLT Survey Telescope (VST)

    NARCIS (Netherlands)

    Iwert, Olaf; Baade, D.; Balestra, A.; Baruffolo, A.; Bortolussi, A.; Christen, F.; Cumani, C.; Deiries, S.; Downing, M.; Geimer, C.; Hess, G.; Hess, J.; Kuijken, K.; Lizon, J.; Muschielok, B.; Nicklas, H.; Reiss, R.; Reyes, J.; Silber, A.; Thillerup, J.; Valentijn, E.; Dorn, David A.; Holland, Andrew D.

    A 16K x 16K, 1 degree x 1 degree field, detector system was developed by ESO for the OmegaCAM instrument for use on the purpose built ESO VLT Survey Telescope (VST). The focal plane consists of an 8 x 4 mosaic of 2K x 4K 15um pixel e2v CCDs and four 2K x 4K CCDs on the periphery for the

  6. "Diversidad de estilos de aprendizaje en el aula de música de ESO" [Diversity of learning styles in the music classrroom of ESO

    Directory of Open Access Journals (Sweden)

    Vicente Simón Simón

    2011-01-01

    Full Text Available RESUMEN:El presente artículo pretende mostrar los resultados de una investigación llevada a cabo en un centro de enseñanzas medias de Castilla-La Mancha, el IES Airén de Tomelloso (Ciudad Real. En él se trata de relacionar los estilos de aprendizaje del alumnado de 1º ciclo de ESO con sus aplicaciones e implicaciones en el campo académico de una materia determinada, la Música. El trabajo se sustenta en los datos obtenidos a través de dos cuestionarios, uno de reconocido prestigio (CHAEA y otro de creación propia (ESTMUS que pretenden determinar las relaciones existentes entre los aprendizajes musicales y los estilos de aprendizaje. Los resultados confirmaron la validez de nuestro cuestionario al reconocerle cerca de un 75 % de coincidencia entre las respuestas de los alumnos de nuestra muestra. Aunque no se observó un estilo de aprendizaje claramente predominante, destacan las personas en las que predomina el estilo de aprendizaje reflexivo en ambos casos.ABSTRACT:This article aims to show the results of research conducted in secondary schools of Castilla-La Mancha, IES Airen of Tomelloso (Ciudad Real. It seeks to relate the learning styles of students of 1 cycle of ESO with its applications and implications in the academic field of a specific subject Music. The work is based on data obtained through two questionnaires, a prestigious (CHAE and one own creation (ESTMUS seeking to determine the relationship between music learning and learning styles. The results confirmed the validity of our questionnaire to recognize about 75% overlap between the responses of students in our sample. Although not observed clearly predominant learning style, outstanding people in the predominantly reflexive learning style in both cases.

  7. A cancer vaccine induces expansion of NY-ESO-1-specific regulatory T cells in patients with advanced melanoma.

    Directory of Open Access Journals (Sweden)

    Lisa M Ebert

    Full Text Available Cancer vaccines are designed to expand tumor antigen-specific T cells with effector function. However, they may also inadvertently expand regulatory T cells (Treg, which could seriously hamper clinical efficacy. To address this possibility, we developed a novel assay to detect antigen-specific Treg based on down-regulation of surface CD3 following TCR engagement, and used this approach to screen for Treg specific to the NY-ESO-1 tumor antigen in melanoma patients treated with the NY-ESO-1/ISCOMATRIX™ cancer vaccine. All patients tested had Treg (CD25(bright FoxP3(+ CD127(neg specific for at least one NY-ESO-1 epitope in the blood. Strikingly, comparison with pre-treatment samples revealed that many of these responses were induced or boosted by vaccination. The most frequently detected response was toward the HLA-DP4-restricted NY-ESO-1(157-170 epitope, which is also recognized by effector T cells. Notably, functional Treg specific for an HLA-DR-restricted epitope within the NY-ESO-1(115-132 peptide were also identified at high frequency in tumor tissue, suggesting that NY-ESO-1-specific Treg may suppress local anti-tumor immune responses. Together, our data provide compelling evidence for the ability of a cancer vaccine to expand tumor antigen-specific Treg in the setting of advanced cancer, a finding which should be given serious consideration in the design of future cancer vaccine clinical trials.

  8. NY-ESO-1- and survivin-specific T-cell responses in the peripheral blood from patients with glioma.

    Science.gov (United States)

    Liu, Zhenjiang; Poiret, Thomas; Persson, Oscar; Meng, Qingda; Rane, Lalit; Bartek, Jiri; Karbach, Julia; Altmannsberger, Hans-Michael; Illies, Christopher; Luo, Xiaohua; Harvey-Peredo, Inti; Jäger, Elke; Dodoo, Ernest; Maeurer, Markus

    2018-02-01

    The prognosis for patients with glioblastoma is grim. Ex vivo expanded tumor-associated antigen (TAA)-reactive T-cells from patients with glioma may represent a viable source for anticancer-directed cellular therapies. Immunohistochemistry was used to test the survivin (n = 40 samples) and NY-ESO-1 (n = 38 samples) protein expression in tumor specimens. T-cells from peripheral blood were stimulated with TAAs (synthetic peptides) in IL-2 and IL-7, or using a combination of IL-2, IL-15 and IL-21. CD4 + and CD8 + T-cells were tested for antigen-specific proliferation by flow cytometry, and IFN-γ production was tested by ELISA. Twenty-eight out of 38 cancer specimens exhibited NY-ESO-1 protein expression, 2/38 showed a strong universal (4+) NY-ESO-1 staining, and 9/40 cancer lesions exhibited a strong (4+) staining for survivin. We could detect antigen-specific IFN-γ responses in 25% blood samples for NY-ESO-1 and 30% for survivin. NY-ESO-1-expanded T-cells recognized naturally processed and presented epitopes. NY-ESO-1 or survivin expression in glioma represents viable targets for anticancer-directed T-cells for the biological therapy of patients with glioma.

  9. Tetramer guided, cell sorter assisted production of clinical grade autologous NY-ESO-1 specific CD8(+) T cells.

    Science.gov (United States)

    Pollack, Seth M; Jones, Robin L; Farrar, Erik A; Lai, Ivy P; Lee, Sylvia M; Cao, Jianhong; Pillarisetty, Venu G; Hoch, Benjamin L; Gullett, Ashley; Bleakley, Marie; Conrad, Ernest U; Eary, Janet F; Shibuya, Kendall C; Warren, Edus H; Carstens, Jason N; Heimfeld, Shelly; Riddell, Stanley R; Yee, Cassian

    2014-01-01

    Adoptive T cell therapy represents an attractive modality for the treatment of patients with cancer. Peripheral blood mononuclear cells have been used as a source of antigen specific T cells but the very low frequency of T cells recognizing commonly expressed antigens such as NY-ESO-1 limit the applicability of this approach to other solid tumors. To overcome this, we tested a strategy combining IL-21 modulation during in vitro stimulation with first-in-class use of tetramer-guided cell sorting to generate NY-ESO-1 specific cytotoxic T lymphocytes (CTL). CTL generation was evaluated in 6 patients with NY-ESO-1 positive sarcomas, under clinical manufacturing conditions and characterized for phenotypic and functional properties. Following in vitro stimulation, T cells stained with NY-ESO-1 tetramer were enriched from frequencies as low as 0.4% to >90% after single pass through a clinical grade sorter. NY-ESO-1 specific T cells were generated from all 6 patients. The final products expanded on average 1200-fold to a total of 36 billion cells, were oligoclonal and contained 67-97% CD8(+), tetramer(+) T cells with a memory phenotype that recognized endogenous NY-ESO-1. This study represents the first series using tetramer-guided cell sorting to generate T cells for adoptive therapy. This approach, when used to target more broadly expressed tumor antigens such as WT-1 and additional Cancer-Testis antigens will enhance the scope and feasibility of adoptive T cell therapy.

  10. A Cancer Vaccine Induces Expansion of NY-ESO-1-Specific Regulatory T Cells in Patients with Advanced Melanoma

    Science.gov (United States)

    Ebert, Lisa M.; MacRaild, Sarah E.; Zanker, Damien; Davis, Ian D.

    2012-01-01

    Cancer vaccines are designed to expand tumor antigen-specific T cells with effector function. However, they may also inadvertently expand regulatory T cells (Treg), which could seriously hamper clinical efficacy. To address this possibility, we developed a novel assay to detect antigen-specific Treg based on down-regulation of surface CD3 following TCR engagement, and used this approach to screen for Treg specific to the NY-ESO-1 tumor antigen in melanoma patients treated with the NY-ESO-1/ISCOMATRIXTM cancer vaccine. All patients tested had Treg (CD25bright FoxP3+ CD127neg) specific for at least one NY-ESO-1 epitope in the blood. Strikingly, comparison with pre-treatment samples revealed that many of these responses were induced or boosted by vaccination. The most frequently detected response was toward the HLA-DP4-restricted NY-ESO-1157–170 epitope, which is also recognized by effector T cells. Notably, functional Treg specific for an HLA-DR-restricted epitope within the NY-ESO-1115–132 peptide were also identified at high frequency in tumor tissue, suggesting that NY-ESO-1-specific Treg may suppress local anti-tumor immune responses. Together, our data provide compelling evidence for the ability of a cancer vaccine to expand tumor antigen-specific Treg in the setting of advanced cancer, a finding which should be given serious consideration in the design of future cancer vaccine clinical trials. PMID:23110239

  11. Immune escape from NY-ESO-1-specific T-cell therapy via loss of heterozygosity in the MHC.

    Science.gov (United States)

    Klippel, Z K; Chou, J; Towlerton, A M; Voong, L N; Robbins, P; Bensinger, W I; Warren, E H

    2014-03-01

    Adoptive immunotherapy of tumors with T cells specific for the cancer-testis antigen NY-ESO-1 has shown great promise in preclinical models and in early stage clinical trials. Tumor persistence or recurrence after NY-ESO-1-specific therapy occurs, however, and the mechanisms of recurrence remain poorly defined. In a murine xenograft model of NY-ESO-1(+) multiple myeloma, we observed tumor recurrence after adoptive transfer of CD8(+) T cells genetically redirected to the prototypic NY-ESO-1157-165 peptide presented by HLA-A*02:01. Analysis of the myeloma cells that had escaped from T-cell control revealed intact expression of NY-ESO-1 and B2M, but selective, complete loss of HLA-A*02:01 expression from the cell surface. Loss of heterozygosity (LOH) in the major histocompatibility complex (MHC) involving the HLA-A locus was identified in the tumor cells, and further analysis revealed selective loss of the allele encoding HLA-A*02:01. Although LOH involving the MHC has not been described in myeloma patients with persistent or recurrent disease after immune therapies such as allogeneic hematopoietic cell transplantation (HCT), it has been described in patients with acute myelogenous leukemia who relapsed after allogeneic HCT. These results suggest that MHC loss should be evaluated in patients with myeloma and other cancers who relapse after adoptive NY-ESO-1-specific T-cell therapy.

  12. Induction of CD8 T-cell responses restricted to multiple HLA class I alleles in a cancer patient by immunization with a 20-mer NY-ESO-1f (NY-ESO-1 91-110) peptide.

    Science.gov (United States)

    Eikawa, Shingo; Kakimi, Kazuhiro; Isobe, Midori; Kuzushima, Kiyotaka; Luescher, Immanuel; Ohue, Yoshihiro; Ikeuchi, Kazuhiro; Uenaka, Akiko; Nishikawa, Hiroyoshi; Udono, Heiichiro; Oka, Mikio; Nakayama, Eiichi

    2013-01-15

    Immunogenicity of a long 20-mer NY-ESO-1f peptide vaccine was evaluated in a lung cancer patient TK-f01, immunized with the peptide with Picibanil OK-432 and Montanide ISA-51. We showed that internalization of the peptide was necessary to present CD8 T-cell epitopes on APC, contrasting with the direct presentation of the short epitope. CD8 T-cell responses restricted to all five HLA class I alleles were induced in the patient after the peptide vaccination. Clonal analysis showed that B*35:01 and B*52:01-restricted CD8 T-cell responses were the two dominant responses. The minimal epitopes recognized by A*24:02, B*35:01, B*52:01 and C*12:02-restricted CD8 T-cell clones were defined and peptide/HLA tetramers were produced. NY-ESO-1 91-101 on A*24:02, NY-ESO-1 92-102 on B*35:01, NY-ESO-1 96-104 on B*52:01 and NY-ESO-1 96-104 on C*12:02 were new epitopes first defined in this study. Identification of the A*24:02 epitope is highly relevant for studying the Japanese population because of its high expression frequency (60%). High affinity CD8 T-cells recognizing tumor cells naturally expressing the epitopes and matched HLA were induced at a significant level. The findings suggest the usefulness of a long 20-mer NY-ESO-1f peptide harboring multiple CD8 T-cell epitopes as an NY-ESO-1 vaccine. Characterization of CD8 T-cell responses in immunomonitoring using peptide/HLA tetramers revealed that multiple CD8 T-cell responses comprised the dominant response. Copyright © 2012 UICC.

  13. Dose-dependent effects of NY-ESO-1 protein vaccine complexed with cholesteryl pullulan (CHP-NY-ESO-1) on immune responses and survival benefits of esophageal cancer patients.

    Science.gov (United States)

    Kageyama, Shinichi; Wada, Hisashi; Muro, Kei; Niwa, Yasumasa; Ueda, Shugo; Miyata, Hiroshi; Takiguchi, Shuji; Sugino, Sahoko H; Miyahara, Yoshihiro; Ikeda, Hiroaki; Imai, Naoko; Sato, Eiichi; Yamada, Tomomi; Osako, Masaharu; Ohnishi, Mami; Harada, Naozumi; Hishida, Tadashi; Doki, Yuichiro; Shiku, Hiroshi

    2013-10-05

    Cholesteryl pullulan (CHP) is a novel antigen delivery system for cancer vaccines. This study evaluated the safety, immune responses and clinical outcomes of patients who received the CHP-NY-ESO-1 complex vaccine, Drug code: IMF-001. Patients with advanced/metastatic esophageal cancer were enrolled and subcutaneously vaccinated with either 100 μg or 200 μg of NY-ESO-1 protein complexed with CHP. The primary endpoints were safety and humoral immune responses, and the secondary endpoint was clinical efficacy. A total of 25 patients were enrolled. Thirteen and twelve patients were repeatedly vaccinated with 100 μg or 200 μg of CHP-NY-ESO-1 with a median of 8 or 9.5 doses, respectively. No serious adverse events related to the vaccine were observed. Three out of 13 patients in the 100-μg cohort and 7 out of 12 patients in the 200-μg cohort were positive for anti-NY-ESO-1 antibodies at baseline. In the 100-μg cohort, an antibody response was observed in 5 out of 10 pre-antibody-negatives patients, and the antibody levels were augmented in 2 pre-antibody-positive patients after vaccination. In the 200-μg cohort, all 5 pre-antibody-negative patients became seropositive, and the antibody level was amplified in all 7 pre-antibody-positive patients. No tumor shrinkage was observed. The patients who received 200 μg of CHP-NY-ESO-1 survived longer than patients receiving 100 μg of CHP-NY-ESO-1, even those who exhibited unresponsiveness to previous therapies or had higher tumor burdens. The safety and immunogenicity of CHP-NY-ESO-1 vaccine were confirmed. The 200 μg dose more efficiently induced immune responses and suggested better survival benefits. (Clinical trial registration number NCT01003808).

  14. [Immune effects of specific CTLs response induced by dendritic cells pulsed with NY-ESO-1 peptide].

    Science.gov (United States)

    Liu, J W; Lu, X; Yang, Z M; Deng, L J; Yang, L

    2017-10-18

    To explore the potential of autologous dendritic cells (DCs) pulsed with caner/testis antigen NY-ESO-1 peptides in inducing specific cytotoxic T lymphocyte (CTLs) response and antineoplastic immune function of specific CTLs. Fifteen patients with II to III stage positive HLA -A0201 + and NY-ESO-1 + were enrolled in the Cancer Hospital Chinese Academy of Medical Sciences on the basis of preclinical experiments from November 2014 to October 2015, and their peripheral blood mononuclear cells (PBMCs) and peripheral blood lymphocytes (PBLs) were isolated. The PBMCs were induced into DCs and pulsed with NY-ESO-1 peptide. The phenotypes of DCs were stained with antibodies against HLA-DR + CD11c + ,CD80 + ,CD83 + and CD86 + , and subsequently analyzed by multichannel flow cytometry (FCM). The killing effects of CTLs pulsed with HLA-A0201-binding peptide NY-ESO-1 and the potential of autologous DCs pulsed with NY-ESO-1 peptides in inducing specific cytotoxic T lymphocytes (CTLs) responses were determined. The patients were administered two infusions of auto-logous CTLs for 1 time every two weeks. The total infusion was with 2 times. The immunological responses and clinical responses were examined in 1 week after the final administration. The immunophenotype of DCs pulsed with NY-ESO-1 peptide was analyzed, HLA-DR + CD11c + cells (93.6%±1.2%), CD80 + cells (87.3%±3.6%), CD83 + cells (82.8%±2.5%) and CD86 + cells (93.4%±6.4%). PBLs isolated from patients primed by DCs pulsed with NY-ESO-1 peptide proliferated continuously and the proliferation index (PI) of the PBLs were analyzed. There was significant difference between the DCs loaded with polypeptides and those unloaded, though it could promote the proliferation of PBLs, but the PI was significantly lower than that of the DCs loaded with NY-ESO-1 peptide (PESO-1 peptides was significantly higher than that in the control group (5.2%±1.2% vs. 0.4%±0.1%). CTLs induced by NY-ESO-1 pulsed DCs exerted a stronger killing

  15. MHC class II/ESO tetramer-based generation of in vitro primed anti-tumor T-helper lines for adoptive cell therapy of cancer.

    Science.gov (United States)

    Poli, Caroline; Raffin, Caroline; Dojcinovic, Danijel; Luescher, Immanuel; Ayyoub, Maha; Valmori, Danila

    2013-02-01

    Generation of tumor-antigen specific CD4(+) T-helper (T(H)) lines through in vitro priming is of interest for adoptive cell therapy of cancer, but the development of this approach has been limited by the lack of appropriate tools to identify and isolate low frequency tumor antigen-specific CD4(+) T cells. Here, we have used recently developed MHC class II/peptide tetramers incorporating an immunodominant peptide from NY-ESO-1 (ESO), a tumor antigen frequently expressed in different human solid and hematologic cancers, to implement an in vitro priming platform allowing the generation of ESO-specific T(H) lines. We isolated phenotypically defined CD4(+) T-cell subpopulations from circulating lymphocytes of DR52b(+) healthy donors by flow cytometry cell sorting and stimulated them in vitro with peptide ESO(119-143), autologous APC and IL-2. We assessed the frequency of ESO-specific cells in the cultures by staining with DR52b/ESO(119-143) tetramers (ESO-tetramers) and TCR repertoire of ESO-tetramer(+) cells by co-staining with TCR variable β chain (BV) specific antibodies. We isolated ESO-tetramer(+) cells by flow cytometry cell sorting and expanded them with PHA, APC and IL-2 to generate ESO-specific T(H) lines. We characterized the lines for antigen recognition, by stimulation with ESO peptide or recombinant protein, cytokine production, by intracellular staining using specific antibodies, and alloreactivity, by stimulation with allo-APC. Using this approach, we could consistently generate ESO-tetramer(+) T(H) lines from conventional CD4(+)CD25(-) naïve and central memory populations, but not from effector memory populations or CD4(+)CD25(+) Treg. In vitro primed T(H) lines recognized ESO with affinities comparable to ESO-tetramer(+) cells from patients immunized with an ESO vaccine and used a similar TCR repertoire. In this study, using MHC class II/ESO tetramers, we have implemented an in vitro priming platform allowing the generation of ESO

  16. Detection of magnetic field in the B2 star ρ Ophiuchi A with ESO FORS2

    Science.gov (United States)

    Pillitteri, I.; Fossati, L.; Castro Rodriguez, N.; Oskinova, L.; Wolk, S. J.

    2018-02-01

    Circumstantial evidence suggests that magnetism and enhanced X-ray emission are likely correlated in early B-type stars: similar fractions of them ( 10%) are strong and hard X-ray sources and possess strong magnetic fields. It is also known that some B-type stars have spots on their surface. Yet up to now no X-ray activity associated with spots on early-type stars was detected. In this Letter we report the detection of a magnetic field on the B2V star ρ Oph A. Previously, we assessed that the X-ray activity of this star is associated with a surface spot, herewith we establish its magnetic origin. We analyze spectra of ρ Oph A obtained with the FORS2 spectrograph at ESO Very Large Telescope (VLT) at two epochs, and detect a longitudinal component of the magnetic field of the order of 500 G in one of the datasets. The detection of the magnetic field only at one epoch can be explained by stellar rotation which is also invoked to explain observed periodic X-ray activity. From archival HARPS ESO VLT high resolution spectra we derived the fundamental stellar parameters of ρ Oph A and further constrained its age. We conclude that ρ Oph A provides strong evidence for the presence of active X-ray emitting regions on young magnetized early type stars. Based on observations collected at the European Organisation for Astronomical Research in the Southern Hemisphere under ESO programmes 099.D-0067(A) and 078.C-0403(A).

  17. Efficient in vivo priming by vaccination with recombinant NY-ESO-1 protein and CpG in antigen naive prostate cancer patients.

    Science.gov (United States)

    Karbach, Julia; Neumann, Antje; Atmaca, Akin; Wahle, Claudia; Brand, Kathrin; von Boehmer, Lotta; Knuth, Alexander; Bender, Armin; Ritter, Gerd; Old, Lloyd J; Jäger, Elke

    2011-02-15

    NY-ESO-1, one of the most immunogenic tumor antigens, is expressed in 15% to 25% of metastatic prostate cancers. The immunological and clinical effects of vaccination with recombinant NY-ESO-1 protein combined with CpG as adjuvant were evaluated. In a phase I clinical study, patients with advanced prostate cancer were vaccinated with recombinant NY-ESO-1 protein (100 μg) mixed with CpG 7909 (2.5 mg) every 3 weeks intradermally for 4 doses. Objectives of the study were the safety of the vaccine and changes of specific humoral and cellular immunological responses to NY-ESO-1 in relation to detectable NY-ESO-1 expression in the individual tumor. All 12 baseline sero-negative patients developed high-titer NY-ESO-1 antibody responses. B-cell epitope mapping identified NY-ESO-1 p91-110 to be recognized most frequently by vaccine-induced antibodies. Two patients developed significant antibody titers against the adjuvant CpG. NY-ESO-1-specific CD4+ and/or CD8+ T-cell responses were induced in 9 patients (69%). Five of these 9 patients did not express NY-ESO-1 in the autologous tumor. Postvaccine CD8+ T-cell clones recognized and lyzed HLA-matched tumor cell lines in an antigen-specific manner. Our data provide clear evidence for the capacity of NY-ESO-1 protein/CpG vaccine to induce integrated antigen-specific immune responses in vivo and to efficiently prime CD8+ T-cell responses in NY-ESO-1 antigen-negative patients. Our results may also support further clinical vaccination protocols with NY-ESO-1 protein not only focused on the treatment of existing cancer, but also to prevent further development of NY-ESO-1 positive cancers in vivo. ©2010 AACR.

  18. The Gaia-ESO Survey: the Galactic thick to thin disc transition

    Science.gov (United States)

    Recio-Blanco, A.; de Laverny, P.; Kordopatis, G.; Helmi, A.; Hill, V.; Gilmore, G.; Wyse, R.; Adibekyan, V.; Randich, S.; Asplund, M.; Feltzing, S.; Jeffries, R.; Micela, G.; Vallenari, A.; Alfaro, E.; Allende Prieto, C.; Bensby, T.; Bragaglia, A.; Flaccomio, E.; Koposov, S. E.; Korn, A.; Lanzafame, A.; Pancino, E.; Smiljanic, R.; Jackson, R.; Lewis, J.; Magrini, L.; Morbidelli, L.; Prisinzano, L.; Sacco, G.; Worley, C. C.; Hourihane, A.; Bergemann, M.; Costado, M. T.; Heiter, U.; Joffre, P.; Lardo, C.; Lind, K.; Maiorca, E.

    2014-07-01

    Aims: The nature of the thick disc and its relation to the thin disc is presently an important subject of debate. In fact, the structural and chemo-dynamical transition between disc populations can be used as a test of the proposed models of Galactic disc formation and evolution. Methods: We used the atmospheric parameters, [α/Fe] abundances, and radial velocities, which were determined from the Gaia-ESO Survey GIRAFFE spectra of FGK-type stars (first nine months of observations) to provide a chemo-kinematical characterisation of the disc stellar populations. We focussed on a subsample of 1016 stars with high-quality parameters, covering the volume | Z | contamination by thin disc stars suggests a gradient up to 64 ± 9 km s-1 dex-1. The distributions of azimuthal velocity, vertical velocity, and orbital parameters are also analysed for the chemically separated samples. Concerning the gradients with galactocentric radius, we find, for the thin disc, a flat behaviour of the azimuthal velocity, a metallicity gradient equal to -0.058 ± 0.008 dex kpc-1 and a very small positive [α/Fe] gradient. For the thick disc, flat gradients in [M/H] and [α/Fe] are derived. Conclusions: Our chemo-kinematical analysis suggests a picture where the thick disc seems to have experienced a settling process, during which its rotation increased progressively and, possibly, the azimuthal velocity dispersion decreased. At [M/H] ≈ -0.25 dex and [α/Fe]≈ 0.1 dex, the mean characteristics of the thick disc in vertical distance to the Galactic plane, rotation, rotational dispersion, and stellar orbits' eccentricity agree with that of the thin disc stars of the same metallicity, suggesting a possible connection between these two populations at a certain epoch of the disc evolution. Finally, the results presented here, based only on the first months of the Gaia ESO Survey observations, confirm how crucial large high-resolution spectroscopic surveys outside the solar neighbourhood are today

  19. IUE observations of the highly luminous Seyfert galaxy ESO 113-IG 45 (Fairall 9)

    Science.gov (United States)

    Masegosa, J.; Moles, M.; Penston, M. V.

    1986-02-01

    Ultraviolet variations of the continuum in ESO 113-IG45 with a doubling time of 50 days indicate a maximum size of 1×1017cm to the emitting region. Power law and blackbody fits to the continuum are both possible. The power-law fit is preferred because of the observed correlation between flux and spectral index which is similar to that found in other Seyferts. The rapid variability is hard to explain with an accretion disc since the variation time-scale is faster than the orbit time in disc models.

  20. Cancer-testis antigen expression in synovial sarcoma: NY-ESO-1, PRAME, MAGEA4, and MAGEA1.

    Science.gov (United States)

    Iura, Kunio; Maekawa, Akira; Kohashi, Kenichi; Ishii, Takeaki; Bekki, Hirofumi; Otsuka, Hiroshi; Yamada, Yuichi; Yamamoto, Hidetaka; Harimaya, Katsumi; Iwamoto, Yukihide; Oda, Yoshinao

    2017-03-01

    Synovial sarcoma (SS) is regarded as a relatively chemosensitive sarcoma, but the prognosis of advanced SSs remains poor. Here we identified highly expressed cancer-testis antigens that could be promising immunotherapy targets for SS, using a previously conducted cDNA microarray, and we assessed the clinicopathological or prognostic relationships of these antigens in SS. We compared the gene expression profiles of 11 SSs with those of 3 normal adipose tissues. Among the up-regulated cancer-testis antigens, we analyzed PRAME, MAGEA1, and MAGEA4 and another cancer-testis antigen (NY-ESO-1) together, by immunohistochemistry and real-time polymerase chain reaction in 108 SSs. Immunohistochemically, NY-ESO-1, PRAME, MAGEA4, and MAGEA1 were positive in 66 (61%), 93 (86%), 89 (82%), and 16 (15%) of 108 SSs, respectively, and 104 (96%) of 108 SSs showed the immunohistochemical expression of at least 1 of NY-ESO-1, PRAME, and MAGEA4. Moreover, the high expression of at least 1 of these 3 antigens was observed in 83% of the SSs. High expression of NY-ESO-1 and MAGEA4 was significantly correlated with the presence of necrosis and advanced clinical stage. The immunohistochemical expression of these cancer-testis antigens was not correlated with prognosis, but the coexpression of NY-ESO-1, PRAME, and MAGEA4 was significantly associated with adverse prognosis. The real-time polymerase chain reaction results were closely related to the immunohistochemical results: NY-ESO-1 (P = .0019), PRAME (P = .039), MAGEA4 (P = .0149), and MAGEA1 (P = .0766). These data support the potential utility of NY-ESO-1, PRAME, and MAGEA4 as immunotherapy targets and ancillary prognostic parameters, suggesting the possible benefit of the combined use of these cancer-testis antigens as an SS immunotherapy target. Copyright © 2016 Elsevier Inc. All rights reserved.

  1. Oncolytic viruses sensitize human tumor cells for NY-ESO-1 tumor antigen recognition by CD4+ effector T cells.

    Science.gov (United States)

    Delaunay, Tiphaine; Violland, Mathilde; Boisgerault, Nicolas; Dutoit, Soizic; Vignard, Virginie; Münz, Christian; Gannage, Monique; Dréno, Brigitte; Vaivode, Kristine; Pjanova, Dace; Labarrière, Nathalie; Wang, Yaohe; Chiocca, E Antonio; Boeuf, Fabrice Le; Bell, John C; Erbs, Philippe; Tangy, Frédéric; Grégoire, Marc; Fonteneau, Jean-François

    2018-01-01

    Oncolytic immunotherapy using oncolytic viruses (OV) has been shown to stimulate the antitumor immune response by inducing the release of tumor-associated antigens (TAA) and danger signals from the dying infected tumor cells. In this study, we sought to determine if the lysis of tumor cells induced by different OV: measles virus, vaccinia virus, vesicular stomatitis virus, herpes simplex type I virus, adenovirus or enterovirus, has consequences on the capacity of tumor cells to present TAA, such as NY-ESO-1. We show that the co-culture of NY-ESO-1 neg /HLA-DP4 pos melanoma cells with NY-ESO-1 pos /HLA-DP4 neg melanoma cells infected and killed by different OV induces an intercellular transfer of NY-ESO-1 that allows the recognition of NY-ESO-1 neg /HLA-DP4 pos tumor cells by an HLA-DP4/NY-ESO-1 (157-170) -specific CD4+ cytotoxic T cell clone, NY67. We then confirmed this result in a second model with an HLA-DP4+ melanoma cell line that expresses a low amount of NY-ESO-1. Recognition of this cell line by the NY67 clone is largely increased in the presence of OV productive infection. Altogether, our results show for the first time another mechanism of stimulation of the anti-tumor immune response by OV, via the loading of tumor cells with TAA that sensitizes them for direct recognition by specific effector CD4+ T cells, supporting the use of OV for cancer immunotherapy.

  2. Combination of autoantibodies against NY-ESO-1 and viral capsid antigen immunoglobulin A for improved detection of nasopharyngeal carcinoma.

    Science.gov (United States)

    Peng, Yu-Hui; Xu, Yi-Wei; Qiu, Si-Qi; Hong, Chao-Qun; Zhai, Tian-Tian; Li, En-Min; Xu, Li-Yan

    2014-09-01

    Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in Southern China and Southeast Asia, and early detection remains a challenge. Autoantibodies have been found to precede the manifestations of symptomatic cancer by several months to years, making their identification of particular relevance for early detection. In the present study, the diagnostic value of serum autoantibodies against NY-ESO-1 in NPC patients was evaluated. The study included 112 patients with NPC and 138 normal controls. Serum levels of autoantibodies against NY-ESO-1 and classical Epstein-Barr virus marker, viral capsid antigen immunoglobulin A (VCA-IgA), were measured by enzyme-linked immunosorbent assay. Measurement of autoantibodies against NY-ESO-1 and VCA-IgA demonstrated a sensitivity/specificity of 42.9/94.9% [95% confidence interval (CI), 33.7-52.6/89.4-97.8%] and 55.4/95.7% (95% CI, 45.7-64.7/90.4-98.2%), respectively. The area under receiver operating characteristic curve for autoantibodies against NY-ESO-1 (0.821; 95% CI, 0.771-0.871) was marginally lower than that for VCA-IgA (0.860; 95% CI, 0.810-0.910) in NPC. The combination of autoantibodies against NY-ESO-1 and VCA-IgA yielded an enhanced sensitivity of 80.4% (95% CI, 71.6-87.0%) and a specificity of 90.6% (95% CI, 84.1-94.7%). Moreover, detection of autoantibodies against NY-ESO-1 could differentiate early-stage NPC patients from normal controls. Our results suggest that autoantibodies against NY-ESO-1 may serve as a potential biomarker, as a supplement to VCA-IgA, for the screening and diagnosis of NPC.

  3. Functional autoantibodies against SSX-2 and NY-ESO-1 in multiple myeloma patients after allogeneic stem cell transplantation.

    Science.gov (United States)

    Luetkens, Tim; Kobold, Sebastian; Cao, Yanran; Ristic, Marina; Schilling, Georgia; Tams, Sinje; Bartels, Britta Marlen; Templin, Julia; Bartels, Katrin; Hildebrandt, York; Yousef, Sara; Marx, Andreas; Haag, Friedrich; Bokemeyer, Carsten; Kröger, Nicolaus; Atanackovic, Djordje

    2014-11-01

    Multiple myeloma (MM) is the malignancy with the most frequent expression of the highly immunogenic cancer-testis antigens (CTA), and we have performed the first analysis of longitudinal expression, immunological properties, and fine specificity of CTA-specific antibody responses in MM. Frequency and characteristics of antibody responses against cancer-testis antigens MAGE-A3, NY-ESO-1, PRAME, and SSX-2 were analyzed using peripheral blood (N = 1094) and bone marrow (N = 200) plasma samples from 194 MM patients. We found that antibody responses against CTA were surprisingly rare, only 2.6 and 3.1 % of patients evidenced NY-ESO-1- and SSX-2-specific antibodies, respectively. NY-ESO-1-specific responses were observed during disease progression, while anti-SSX-2 antibodies appeared after allogeneic stem cell transplantation and persisted during clinical remission. We found that NY-ESO-1- and SSX-2-specific antibodies were both capable of activating complement and increasing CTA uptake by antigen-presenting cells. SSX-2-specific antibodies were restricted to IgG3, NY-ESO-1 responses to IgG1 and IgG3. Remarkably, NY-ESO-1-positive sera recognized various non-contiguous regions, while SSX-2-specific responses were directed against a single 6mer epitope, SSX-2(85-90). We conclude that primary autoantibodies against intracellular MM-specific tumor antigens SSX-2 and NY-ESO-1 are rare but functional. While their contribution to disease control still remains unclear, our data demonstrate their theoretic ability to affect cellular anti-tumor immunity by formation and uptake of mono- and polyvalent immune complexes.

  4. The Gaia-ESO Survey: matching chemodynamical simulations to observations of the Milky Way

    Science.gov (United States)

    Thompson, B. B.; Few, C. G.; Bergemann, M.; Gibson, B. K.; MacFarlane, B. A.; Serenelli, A.; Gilmore, G.; Randich, S.; Vallenari, A.; Alfaro, E. J.; Bensby, T.; Francois, P.; Korn, A. J.; Bayo, A.; Carraro, G.; Casey, A. R.; Costado, M. T.; Donati, P.; Franciosini, E.; Frasca, A.; Hourihane, A.; Jofré, P.; Hill, V.; Heiter, U.; Koposov, S. E.; Lanzafame, A.; Lardo, C.; de Laverny, P.; Lewis, J.; Magrini, L.; Marconi, G.; Masseron, T.; Monaco, L.; Morbidelli, L.; Pancino, E.; Prisinzano, L.; Recio-Blanco, A.; Sacco, G.; Sousa, S. G.; TautvaišienÄ--, G.; Worley, C. C.; Zaggia, S.

    2018-01-01

    The typical methodology for comparing simulated galaxies with observational surveys is usually to apply a spatial selection to the simulation to mimic the region of interest covered by a comparable observational survey sample. In this work, we compare this approach with a more sophisticated post-processing in which the observational uncertainties and selection effects (photometric, surface gravity and effective temperature) are taken into account. We compare a 'solar neighbourhood analogue' region in a model Milky Way-like galaxy simulated with RAMSES-CH with fourth release Gaia-ESO survey data. We find that a simple spatial cut alone is insufficient and that the observational uncertainties must be accounted for in the comparison. This is particularly true when the scale of uncertainty is large compared to the dynamic range of the data, e.g. in our comparison, the [Mg/Fe] distribution is affected much more than the more accurately determined [Fe/H] distribution. Despite clear differences in the underlying distributions of elemental abundances between simulation and observation, incorporating scatter to our simulation results to mimic observational uncertainty produces reasonable agreement. The quite complete nature of the Gaia-ESO survey means that the selection function has minimal impact on the distribution of observed age and metal abundances but this would become increasingly more important for surveys with narrower selection functions.

  5. Decitabine immunosensitizes human gliomas to NY-ESO-1 specific T lymphocyte targeting through the Fas/Fas Ligand pathway

    Directory of Open Access Journals (Sweden)

    Konkankit Veerauo V

    2011-11-01

    Full Text Available Abstract Background The lack of effective treatments for gliomas makes them a significant health problem and highlights the need for the development of novel and innovative treatment approaches. Immunotherapy is an appealing strategy because of the potential ability for immune cells to traffic to and destroy infiltrating tumor cells. However, the absence of well-characterized, highly immunogenic tumor-rejection antigens (TRA in gliomas has limited the implementation of targeted immune-based therapies. Methods We hypothesized that treatment with the demethylating agent, decitabine, would upregulate the expression of TRA on tumor cells, thereby facilitating enhanced surveillance by TRA-specific T cells. Results and Discussion Treatment of human glioma cells with decitabine increased the expression of NY-ESO-1 and other well characterized cancer testes antigens. The upregulation of NY-ESO-1 made these tumors susceptible to NY-ESO-1-specific T-cell recognition and lysis. Interestingly, decitabine treatment of T98 glioma cells also sensitized them to Fas-dependent apoptosis with an agonistic antibody, while a Fas blocking antibody could largely prevent the enhanced functional recognition by NY-ESO-1 specific T cells. Thus, decitabine treatment transformed a non-immunogenic glioma cell into an immunogenic target that was efficiently recognized by NY-ESO-1--specific T cells. Conclusions Such data supports the hypothesis that agents which alter epigenetic cellular processes may "immunosensitize" tumor cells to tumor-specific T cell-mediated lysis.

  6. MAGE-A is More Highly Expressed Than NY-ESO-1 in a Systematic Immunohistochemical Analysis of 3668 Cases.

    Science.gov (United States)

    Kerkar, Sid P; Wang, Zeng-Feng; Lasota, Jerzy; Park, Tristen; Patel, Krishna; Groh, Eric; Rosenberg, Steven A; Miettinen, Markku M

    2016-05-01

    Two cancer testis antigens, the New York esophageal squamous cell carcinoma-1 (NY-ESO-1) and the melanoma-antigen family A (MAGE-A), represent promising immunotherapy targets due to the low expression of these antigens in nonmalignant tissue. To assess overexpression patterns in various cancers, we performed a systematic immunohistochemical analysis for NY-ESO-1 and MAGE-A on tissue array samples of 3668 common epithelial carcinomas (CA) and germ cell tumors of high prevalence and mortality. Here, we find significantly higher expression of MAGE-A (>50% on tumor cells) compared with NY-ESO-1 in several CAs including cutaneous squamous cell carcinomas (SCC) (52.8%/2.8%), esophageal SCC (50%/0%), head and neck SCC (41.1%/ESO-1, was seen in whole slide evaluations of an independent cohort of metastatic SCC (45.5%/3.6%) and metastatic CA (13.5%/0%) of various primaries with significantly higher expression of MAGE-A in metastatic SCC compared with other metastatic CA. MAGE-A is also more highly expressed in germ cell tumors, seminomas (69%/3.5%) and nonseminomas (40.1%/4.7%). In summary, MAGE-A is more highly expressed than NY-ESO-1 in a majority of human malignancies, and targeting MAGE-A may benefit a large number of patients.

  7. Decitabine immunosensitizes human gliomas to NY-ESO-1 specific T lymphocyte targeting through the Fas/Fas Ligand pathway

    Science.gov (United States)

    2011-01-01

    Background The lack of effective treatments for gliomas makes them a significant health problem and highlights the need for the development of novel and innovative treatment approaches. Immunotherapy is an appealing strategy because of the potential ability for immune cells to traffic to and destroy infiltrating tumor cells. However, the absence of well-characterized, highly immunogenic tumor-rejection antigens (TRA) in gliomas has limited the implementation of targeted immune-based therapies. Methods We hypothesized that treatment with the demethylating agent, decitabine, would upregulate the expression of TRA on tumor cells, thereby facilitating enhanced surveillance by TRA-specific T cells. Results and Discussion Treatment of human glioma cells with decitabine increased the expression of NY-ESO-1 and other well characterized cancer testes antigens. The upregulation of NY-ESO-1 made these tumors susceptible to NY-ESO-1-specific T-cell recognition and lysis. Interestingly, decitabine treatment of T98 glioma cells also sensitized them to Fas-dependent apoptosis with an agonistic antibody, while a Fas blocking antibody could largely prevent the enhanced functional recognition by NY-ESO-1 specific T cells. Thus, decitabine treatment transformed a non-immunogenic glioma cell into an immunogenic target that was efficiently recognized by NY-ESO-1--specific T cells. Conclusions Such data supports the hypothesis that agents which alter epigenetic cellular processes may "immunosensitize" tumor cells to tumor-specific T cell-mediated lysis. PMID:22060015

  8. Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells.

    Science.gov (United States)

    Mobergslien, Anne; Vasovic, Vlada; Mathiesen, Geir; Fredriksen, Lasse; Westby, Phuong; Eijsink, Vincent G H; Peng, Qian; Sioud, Mouldy

    2015-01-01

    Given their safe use in humans and inherent adjuvanticity, Lactic Acid Bacteria may offer several advantages over other mucosal delivery strategies for cancer vaccines. The objective of this study is to evaluate the immune responses in mice after oral immunization with Lactobacillus (L) plantarum WCFS1 expressing a cell-wall anchored tumor antigen NY-ESO-1. And to investigate the immunostimulatory potency of this new candidate vaccine on human dendritic cells (DCs). L. plantarum displaying NY-ESO-1 induced NY-ESO-1 specific antibodies and T-cell responses in mice. By contrast, L. plantarum displaying conserved proteins such as heat shock protein-27 and galectin-1, did not induce immunity, suggesting that immune tolerance to self-proteins cannot be broken by oral administration of L. plantarum. With respect to immunomodulation, immature DCs incubated with wild type or L. plantarum-NY-ESO-1 upregulated the expression of co-stimulatory molecules and secreted a large amount of interleukin (IL)-12, TNF-α, but not IL-4. Moreover, they upregulated the expression of immunosuppressive factors such as IL-10 and indoleamine 2,3-dioxygenase. Although L. plantarum-matured DCs expressed inhibitory molecules, they stimulated allogeneic T cells in-vitro. Collectively, the data indicate that L. plantarum-NY-ESO-1 can evoke antigen-specific immunity upon oral administration and induce DC maturation, raising the potential of its use in cancer immunotherapies.

  9. N° 15-2000: ESA, CERN and ESO launch "Physics on Stage"

    Science.gov (United States)

    2000-03-01

    But how much do the citizens of Europe really know about physics? Here is a unique opportunity to learn more about this elusive subject! Beginning in February 2000, three major European research establishments [1] are organising a unique Europe-wide programme to raise the public awareness of physics and related sciences. "Physics on Stage" is launched by the European Space Agency (ESA), the European Laboratory for Particle Physics (CERN), and the European Southern Observatory (ESO), with support from the European Union (EU). Other partners include the European Physical Society (EPS) and the European Association for Astronomy Education (EAAE). This exciting programme is part of the European Week for Science and Technology and will culminate in a Science Festival during November 6-11, 2000, at CERN, Geneva. Why "Physics on Stage"? The primary goal of "Physics on Stage" is to counteract the current decline in interest and knowledge of physics among Europe's citizens by means of a series of highly visible promotional activities. It will bring together leading scientists and educators, government bodies and the media, to confront the diminishing attraction of physics to young people and to develop strategies to reverse this trend. The objective in the short term is to infuse excitement and to provide new educational materials. In the longer term, "Physics on Stage" will generate new developments by enabling experts throughout Europe to meet, exchange and innovate. "Physics on Stage" in 22 European Countries. "Physics on Stage" has been initiated in 22 European countries [2]. In each country, a dedicated National Steering Committee (NSC) is being formed which will be responsible for their own national programme. A list of contact addresses is attached below. "Physics on Stage" is based on a series of high-profile physics-related activities that will inform the European public in general, and European high school physics teachers and media representatives in particular

  10. Expression of NY-ESO-1 in Triple-Negative Breast Cancer Is Associated with Tumor-Infiltrating Lymphocytes and a Good Prognosis.

    Science.gov (United States)

    Lee, Hee Jin; Kim, Joo Young; Song, In Hye; Park, In Ah; Yu, Jong Han; Gong, Gyungyub

    2015-01-01

    Accumulating evidence suggests that immunotherapy has great potential for treating triple-negative breast cancer (TNBC). We analyzed the expression of NY-ESO-1, which is a potent immunogenic cancer testis antigen, and its association with clinicopathological factors in large cohorts of breast cancer patients. A total of 623 consecutive breast cancer patients who underwent surgery between 1993 and 1998 and 612 TNBC patients who underwent surgery between 2004 and 2010 at Asan Medical Center were included. Immunohistochemical staining for NY-ESO-1 was performed using tissue microarrays. NY-ESO-1 was expressed in 2.6% of consecutive breast cancers, all of which were TNBC (p ESO-1 expression was identified in 9.7% of the TNBC cohort and was significantly correlated with a higher level of tumor-infiltrating lymphocytes (TIL; p = 0.026). In survival analyses, a lower level of TIL (all, p ESO-1 expression (p = 0.024) were significantly associated with poor disease-free survival. Additionally, positive NY-ESO-1 expression was an independent favorable prognostic factor in TNBC patients (p = 0.046). NY-ESO-1 is specifically expressed in TNBC, and NY-ESO-1 expression is an independent good prognostic factor in TNBC. Evaluation of NY-ESO-1 expression in TNBC might be useful for selecting patients who may benefit from vaccination therapy and also has a prognostic significance in TNBC. © 2015 S. Karger AG, Basel.

  11. MAGE-A1, GAGE and NY-ESO-1 cancer/testis antigen expression during human gonadal development

    DEFF Research Database (Denmark)

    Gjerstorff, Morten F; Kock, Kirsten; Nielsen, Ole

    2007-01-01

    BACKGROUND: Cancer/testis antigens (CTAs) are expressed in several cancers and during normal adult male germ cell differentiation. Little is known about their role in fetal development of human germ cells. METHODS: We examined expression of the CTAs MAGE-A1, GAGE and NY-ESO-1 in fetal gonads...... with highly proliferating germ cells, whereas GAGE proteins have a more general function in germ cells unrelated to any specific developmental stage. The recognition of differential cellular expression of GAGE, MAGE-A1, NY-ESO-1 and OCT4 may help define biologically distinct germ cell subpopulations....... cells and the staining intensity of all three CTAs peaked during the second trimester and gradually decreased towards birth in both male and female germ cells. In oocytes, MAGE-A1 expression terminated around birth, whereas NY-ESO-1 expression persisted through the neonatal stage and GAGE expression...

  12. Hubble and ESO's VLT provide unique 3D views of remote galaxies

    Science.gov (United States)

    2009-03-01

    Astronomers have obtained exceptional 3D views of distant galaxies, seen when the Universe was half its current age, by combining the twin strengths of the NASA/ESA Hubble Space Telescope's acute eye, and the capacity of ESO's Very Large Telescope to probe the motions of gas in tiny objects. By looking at this unique "history book" of our Universe, at an epoch when the Sun and the Earth did not yet exist, scientists hope to solve the puzzle of how galaxies formed in the remote past. ESO PR Photo 10a/09 A 3D view of remote galaxies ESO PR Photo 10b/09 Measuring motions in 3 distant galaxies ESO PR Video 10a/09 Galaxies in collision For decades, distant galaxies that emitted their light six billion years ago were no more than small specks of light on the sky. With the launch of the Hubble Space Telescope in the early 1990s, astronomers were able to scrutinise the structure of distant galaxies in some detail for the first time. Under the superb skies of Paranal, the VLT's FLAMES/GIRAFFE spectrograph (ESO 13/02) -- which obtains simultaneous spectra from small areas of extended objects -- can now also resolve the motions of the gas in these distant galaxies (ESO 10/06). "This unique combination of Hubble and the VLT allows us to model distant galaxies almost as nicely as we can close ones," says François Hammer, who led the team. "In effect, FLAMES/GIRAFFE now allows us to measure the velocity of the gas at various locations in these objects. This means that we can see how the gas is moving, which provides us with a three-dimensional view of galaxies halfway across the Universe." The team has undertaken the Herculean task of reconstituting the history of about one hundred remote galaxies that have been observed with both Hubble and GIRAFFE on the VLT. The first results are coming in and have already provided useful insights for three galaxies. In one galaxy, GIRAFFE revealed a region full of ionised gas, that is, hot gas composed of atoms that have been stripped of

  13. The Gaia-ESO Survey: Low-α element stars in the Galactic bulge

    Science.gov (United States)

    Recio-Blanco, A.; Rojas-Arriagada, A.; de Laverny, P.; Mikolaitis, S.; Hill, V.; Zoccali, M.; Fernández-Trincado, J. G.; Robin, A. C.; Babusiaux, C.; Gilmore, G.; Randich, S.; Alfaro, E.; Allende Prieto, C.; Bragaglia, A.; Carraro, G.; Jofré, P.; Lardo, C.; Monaco, L.; Morbidelli, L.; Zaggia, S.

    2017-06-01

    We take advantage of the Gaia-ESO Survey iDR4 bulge data to search for abundance anomalies that could shed light on the composite nature of the Milky Way bulge. The α-element (Mg, Si, and whenever available, Ca) abundances, and their trends with Fe abundances have been analysed for a total of 776 bulge stars. In addition, the aluminum abundances and their ratio to Fe and Mg have also been examined. Our analysis reveals the existence of low-α element abundance stars with respect to the standard bulge sequence in the [α/ Fe] versus [Fe/H] plane. Eighteen objects present deviations in [α/ Fe] ranging from 2.1 to 5.3σ with respect to the median standard value. Those stars do not show Mg-Al anti-correlation patterns. Incidentally, this sign of the existence of multiple stellar populations is reported firmly for the first time for the bulge globular cluster NGC 6522. The identified low-α abundance stars have chemical patterns that are compatible with those of the thin disc. Their link with massive dwarf galaxies accretion seems unlikely, as larger deviations in α abundance and Al would be expected. The vision of a bulge composite nature and a complex formation process is reinforced by our results. The approach used, which is a multi-method and model-driven analysis of high resolution data, seems crucial to reveal this complexity. Based on data products from observations made with ESO Telescopes at the La Silla Paranal Observatory under programme ID 188.B-3002. These data products have been processed by the Cambridge Astronomy Survey Unit (CASU) at the Institute of Astronomy, University of Cambridge, and by the FLAMES/UVES reduction team at INAF/Osservatorio Astrofisico di Arcetri. These data have been obtained from the Gaia-ESO Survey Data Archive, and prepared and hosted by the Wide Field Astronomy Unit, Institute for Astronomy, University of Edinburgh, which is funded by the UK Science and Technology Facilities Council.

  14. Resiquimod as an immunologic adjuvant for NY-ESO-1 protein vaccination in patients with high-risk melanoma.

    Science.gov (United States)

    Sabado, Rachel Lubong; Pavlick, Anna; Gnjatic, Sacha; Cruz, Crystal M; Vengco, Isabelita; Hasan, Farah; Spadaccia, Meredith; Darvishian, Farbod; Chiriboga, Luis; Holman, Rose Marie; Escalon, Juliet; Muren, Caroline; Escano, Crystal; Yepes, Ethel; Sharpe, Dunbar; Vasilakos, John P; Rolnitzsky, Linda; Goldberg, Judith; Mandeli, John; Adams, Sylvia; Jungbluth, Achim; Pan, Linda; Venhaus, Ralph; Ott, Patrick A; Bhardwaj, Nina

    2015-03-01

    The Toll-like receptor (TLR) 7/8 agonist resiquimod has been used as an immune adjuvant in cancer vaccines. We evaluated the safety and immunogenicity of the cancer testis antigen NY-ESO-1 given in combination with Montanide (Seppic) with or without resiquimod in patients with high-risk melanoma. In part I of the study, patients received 100 μg of full-length NY-ESO-1 protein emulsified in 1.25 mL of Montanide (day 1) followed by topical application of 1,000 mg of 0.2% resiquimod gel on days 1 and 3 (cohort 1) versus days 1, 3, and 5 (cohort 2) of a 21-day cycle. In part II, patients were randomized to receive 100-μg NY-ESO-1 protein plus Montanide (day 1) followed by topical application of placebo gel [(arm A; n = 8) or 1,000 mg of 0.2% resiquimod gel (arm B; n = 12)] using the dosing regimen established in part I. The vaccine regimens were generally well tolerated. NY-ESO-1-specific humoral responses were induced or boosted in all patients, many of whom had high titer antibodies. In part II, 16 of 20 patients in both arms had NY-ESO-1-specific CD4⁺ T-cell responses. CD8⁺ T-cell responses were only seen in 3 of 12 patients in arm B. Patients with TLR7 SNP rs179008 had a greater likelihood of developing NY-ESO-1-specific CD8⁺ responses. In conclusion, NY-ESO-1 protein in combination with Montanide with or without topical resiquimod is safe and induces both antibody and CD4⁺ T-cell responses in the majority of patients; the small proportion of CD8⁺ T-cell responses suggests that the addition of topical resiquimod to Montanide is not sufficient to induce consistent NY-ESO-1-specific CD8⁺ T-cell responses. ©2015 American Association for Cancer Research.

  15. Measurement of serum antibodies against NY-ESO-1 by ELISA: A guide for the treatment of specific immunotherapy for patients with advanced colorectal cancer.

    Science.gov (United States)

    Long, Yan-Yan; Wang, Yu; Huang, Qian-Rong; Zheng, Guang-Shun; Jiao, Shun-Chang

    2014-10-01

    NY-ESO-1 has been identified as one of the most immunogenic antigens; thus, is a highly attractive target for cancer immunotherapy. The present study analyzed the expression of serum antibodies (Abs) against NY-ESO-1 in patients with advanced colorectal cancer (CRC), with the aim of guiding the treatment of NY-ESO-1-based specific-immunotherapy for these patients. Furthermore, the present study was the first to evaluate the kinetic expression of anti-NY-ESO-1 Abs and investigate the possible influencing factors. A total of 239 serum samples from 155 pathologically confirmed patients with advanced CRC (stages III and IV) were collected. The presence of spontaneous Abs against NY-ESO-1 was analyzed using an enzyme-linked immunosorbent assay (ELISA). The results demonstrated that 24.5% (38/155) of the investigated patients were positive for NY-ESO-1-specific Abs. No statistically significant correlations were identified between the expression of anti-NY-ESO-1 Abs and clinicopathological parameters, including age and gender, location, grading, local infiltration, lymph node status, metastatic status and K-ras mutation status (P>0.05). In 59 patients, the kinetic expression of anti-NY-ESO-1 Abs was analyzed, of which 14 patients were initially positive and 45 patients were initially negative. Notably, 16/59 (27.1%) patients changed their expression status during the study period, and the initially positive patients were more likely to change compared with the initially negative patients (85.7 vs. 8.8%; PESO-1 by ELISA is an easy and feasible method. The high expression rate of NY-ESO-1-specific Abs in CRC patients indicates that measuring the levels of serum Abs against NY-ESO-1 may guide the treatment of NY-ESO-1-based specific immunotherapy for patients with advanced CRC.

  16. Integrated NY-ESO-1 antibody and CD8+ T-cell responses correlate with clinical benefit in advanced melanoma patients treated with ipilimumab.

    Science.gov (United States)

    Yuan, Jianda; Adamow, Matthew; Ginsberg, Brian A; Rasalan, Teresa S; Ritter, Erika; Gallardo, Humilidad F; Xu, Yinyan; Pogoriler, Evelina; Terzulli, Stephanie L; Kuk, Deborah; Panageas, Katherine S; Ritter, Gerd; Sznol, Mario; Halaban, Ruth; Jungbluth, Achim A; Allison, James P; Old, Lloyd J; Wolchok, Jedd D; Gnjatic, Sacha

    2011-10-04

    Ipilimumab, a monoclonal antibody against cytotoxic T lymphocyte antigen 4 (CTLA-4), has been shown to improve survival in patients with advanced metastatic melanoma. It also enhances immunity to NY-ESO-1, a cancer/testis antigen expressed in a subset of patients with melanoma. To characterize the association between immune response and clinical outcome, we first analyzed NY-ESO-1 serum antibody by ELISA in 144 ipilimumab-treated patients with melanoma and found 22 of 140 (16%) seropositive at baseline and 31 of 144 (22%) seropositive following treatment. These NY-ESO-1-seropositive patients had a greater likelihood of experiencing clinical benefit 24 wk after ipilimumab treatment than NY-ESO-1-seronegative patients (P = 0.02, relative risk = 1.8, two-tailed Fisher test). To understand why some patients with NY-ESO-1 antibody failed to experience clinical benefit, we analyzed NY-ESO-1-specific CD4(+) and CD8(+) T-cell responses by intracellular multicytokine staining in 20 NY-ESO-1-seropositive patients and found a surprising dissociation between NY-ESO-1 antibody and CD8 responses in some patients. NY-ESO-1-seropositive patients with associated CD8(+) T cells experienced more frequent clinical benefit (10 of 13; 77%) than those with undetectable CD8(+) T-cell response (one of seven; 14%; P = 0.02; relative risk = 5.4, two-tailed Fisher test), as well as a significant survival advantage (P = 0.01; hazard ratio = 0.2, time-dependent Cox model). Together, our data suggest that integrated NY-ESO-1 immune responses may have predictive value for ipilimumab treatment and argue for prospective studies in patients with established NY-ESO-1 immunity. The current findings provide a strong rationale for the clinical use of modulators of immunosuppression with concurrent approaches to favor tumor antigen-specific immune responses, such as vaccines or adoptive transfer, in patients with cancer.

  17. Evaluation of LAGE-1 and NY-ESO-1 expression in multiple myeloma patients to explore possible benefits of their homology for immunotherapy.

    Science.gov (United States)

    de Carvalho, Fabricio; Vettore, Andre L; Inaoka, Riguel J; Karia, Bruno; Andrade, Valéria C C; Gnjatic, Sacha; Jungbluth, Achim A; Colleoni, Gisele W B

    2011-01-20

    Due to the high homology between the LAGE-1 and NY-ESO-1 proteins, we hypothesized that an anti-NY-ESO-1 vaccine might elicit LAGE-1 immunity and hence may be effective in multiple myeloma (MM) patients with LAGE-1-positive/NY-ESO-1-negative tumors. Therefore, we set out to evaluate LAGE-1 and NY-ESO-1 mRNA and protein expression in MM patients in a bid to evaluate possible benefits of their homology for immunotherapy. LAGE-1 (a and b isoforms) and NY-ESO-1 mRNA expression was studied in 18 normal tissues and 50 bone marrow MM samples by RT-PCR. LAGE-1 and NY-ESO-1 protein expression was analyzed by immunohistochemistry (IHC) in 27 MM specimens using mAbs 219-510-23 and E978. Spontaneous serological immune response against both antigens was analyzed by ELISA in sera from 33 MM patients. LAGE-1 (a and b isoforms) was positive in 42% and NY-ESO-1 in 26% of the MM samples analyzed by RT-PCR. Both genes were found to be expressed in 18% of the cases, while at least one of the genes was found to be expressed in 50% of the cases. In LAGE-1 positive samples, 81% were positive for LAGE-1a and 19% were positive for both LAGE-1a and -1b. LAGE-1 and NY-ESO-1 protein expression could only be detected in two cases by IHC and there was a clear strong spontaneous antibody response to LAGE-1 and NY-ESO-1 in only one MM patient. In conclusion, LAGE-1a and NY-ESO-1 homology cannot be easily exploited in an anti-NY-ESO-1 vaccine given the low frequency of protein expression detected by IHC or serum analysis.

  18. The AMBRE project: Results from the MATISSE analysis of the ESO-FEROS archived spectra

    Directory of Open Access Journals (Sweden)

    Hill V.

    2012-02-01

    Full Text Available The goal of AMBRE, a joint project between ESO and the Observatoire de la Cote d’Azur, is to provide a homogeneous determination of the stellar parameters (including mean metallicity and some chemical abundances for the archived spectra of the FEROS, HARPS, UVES and Flames/GIRAFFE spectrographs. We present here a preliminary analysis of the FEROS stellar sample. The stellar parameters, derived using the stellar classification algorithm MATISSE, have been combined with distances and radial velocities in order to explore current theories regarding galactic stellar populations. We have reviewed the sample for potential disk stars with which we can explore kinematic and chemical signatures of the thin and thick disk. We present here a comparison of the potential disk stellar sample to the analysis of 66 disk stars that was carried out in.

  19. VizieR Online Data Catalog: Gaia-ESO Survey: Halpha emission stars catalogue (Traven+, 2015)

    Science.gov (United States)

    Traven, G.; Zwitter, T.; van Eck, S.; Klutsch, A.; Bonito, R.; Lanzafame, A. C.; Alfaro, E. J.; Bayo, A.; Bragaglia, A.; Costado, M. T.; Damiani, F.; Flaccomio, E.; Frasca, A.; Hourihane, A.; Jimenez-Esteban, F.; Lardo, C.; Morbidelli, L.; Pancino, E.; Prisinzano, L.; Sacco, G. G.; Worley, C. C.

    2015-07-01

    The GES employs the VLT-UT2 FLAMES high-resolution multiobject spectrograph . FLAMES has two instruments, the higher-resolution UVES, fed by 8 fibres, and GIRAFFE, with about 130 fibres. In the present paper we concentrate on the much more numerous GIRAFFE spectra to thoroughly test our classification scheme; a similar analysis of the UVES spectra will follow later. We use only spectra obtained in the wavelength range with Hα line - HR15N setting (6470-6790Å, R=17000), for which the majority of targets selected by GES are open cluster stars. Along with ongoing GES observations, we also use all relevant ESO archive data, which are included to be analysed as part of GES. (3 data files).

  20. The Gaia-ESO Survey: Galactic evolution of sulphur and zinc

    Science.gov (United States)

    Duffau, S.; Caffau, E.; Sbordone, L.; Bonifacio, P.; Andrievsky, S.; Korotin, S.; Babusiaux, C.; Salvadori, S.; Monaco, L.; François, P.; Skúladóttir, Á.; Bragaglia, A.; Donati, P.; Spina, L.; Gallagher, A. J.; Ludwig, H.-G.; Christlieb, N.; Hansen, C. J.; Mott, A.; Steffen, M.; Zaggia, S.; Blanco-Cuaresma, S.; Calura, F.; Friel, E.; Jiménez-Esteban, F. M.; Koch, A.; Magrini, L.; Pancino, E.; Tang, B.; Tautvaišienė, G.; Vallenari, A.; Hawkins, K.; Gilmore, G.; Randich, S.; Feltzing, S.; Bensby, T.; Flaccomio, E.; Smiljanic, R.; Bayo, A.; Carraro, G.; Casey, A. R.; Costado, M. T.; Damiani, F.; Franciosini, E.; Hourihane, A.; Jofré, P.; Lardo, C.; Lewis, J.; Morbidelli, L.; Sousa, S. G.; Worley, C. C.

    2017-08-01

    Context. Due to their volatile nature, when sulphur and zinc are observed in external galaxies, their determined abundances represent the gas-phase abundances in the interstellar medium. This implies that they can be used as tracers of the chemical enrichment of matter in the Universe at high redshift. Comparable observations in stars are more difficult and, until recently, plagued by small number statistics. Aims: We wish to exploit the Gaia-ESO Survey (GES) data to study the behaviour of sulphur and zinc abundances of a large number of Galactic stars, in a homogeneous way. Methods: By using the UVES spectra of the GES sample, we are able to assemble a sample of 1301 Galactic stars, including stars in open and globular clusters in which both sulphur and zinc were measured. Results: We confirm the results from the literature that sulphur behaves as an α-element. We find a large scatter in [Zn/Fe] ratios among giant stars around solar metallicity. The lower ratios are observed in giant stars at Galactocentric distances less than 7.5 kpc. No such effect is observed among dwarf stars, since they do not extend to that radius. Conclusions: Given the sample selection, giants and dwarfs are observed at different Galactic locations, and it is plausible, and compatible with simple calculations, that Zn-poor giants trace a younger population more polluted by SN Ia yields. It is necessary to extend observations in order to observe both giants and dwarfs at the same Galactic location. Further theoretical work on the evolution of zinc is also necessary. Based on observations collected at the European Organisation for Astronomical Research in the Southern Hemisphere under ESO programmes 188.B-3002, 193.B-0936.The full table of S abundances is only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/604/A128

  1. Live Webcasts from CERN and ESO for European Science and Technology Week

    Science.gov (United States)

    2002-10-01

    Visit http://www.cern.ch/sci-tech on 7 - 8 November to find out what modern Europeans can't live without. Seven of Europe's leading Research Organizations [1] are presenting three live Webcasts from CERN in a joint outreach programme for the European Science and Technology Week . The aim of Sci-Tech... couldn't be without it! is to show how today's society couldn't be without cutting-edge scientific research. See also ESO Press Release 05/02. Northern Europeans can't imagine their households without ovens, whereas Southern Europeans identify the refrigerator as the most essential household appliance. In the area of communications, cars and motorbikes are clearly the technologies of choice in Italy, but are regarded as less important in countries like Norway and Germany. For entertainment, the personal computer is a clear winner as the device is considered most essential by all Europeans, followed by the TV and the Internet. This hit parade of technological marvels is the result of a phone and online survey conducted by the Sci-Tech... couldn't be without it! team for this year's European Science and Technology Week on 4-10 November. The technologies Europeans could not be without, form the starting point of three entertaining and informative Webcast shows in Italian (Thursday 7 November at 10:00 CET), French (Thursday 7 November at 15:00 CET) and English (Friday 8 November at 15:00 CET), broadcast live on the Internet from a studio at CERN. During these Webcasts scientists from the seven research Organizations and their industrial partners Sun Microsystems, Siemens, L'Oreal and Luminex will engage - from the CERN studio or from remote locations through teleconference links - an audience of Internauts all over the world. The public will be taken inside their most popular gadgets to discover the science that made them possible and how vital fundamental research has been in the creation of modern technology. Fundamental science will be brought as close as possible to

  2. Antigen-specific in vitro expansion of functional redirected NY-ESO-1-specific human CD8+ T-cells in a cell-free system.

    Science.gov (United States)

    Jakka, Gopinadh; Schuberth, Petra C; Thiel, Markus; Held, Gerhard; Stenner, Frank; Van Den Broek, Maries; Renner, Christoph; Mischo, Axel; Petrausch, Ulf

    2013-10-01

    Tumors can be targeted by the adoptive transfer of chimeric antigen receptor (CAR) redirected T-cells. Antigen-specific expansion protocols are needed to generate large quantities of redirected T-cells. We aimed to establish a protocol to expand functional active NY-ESO-1-specific redirected human CD8(+) T-cells. The anti-idiotypic Fab antibody A4 with specificity for HLA-A 0201/NY-ESO-1157-165 was tested by competition assays using a HLA-A 0201/NY-ESO-1157-165 tetramer. HLA-A 0201/NY-ESO-1157-165 redirected T-cells were generated, expanded and tested for CAR expression, cytokine release, in vitro cytolysis and protection against xenografted HLA-A 0201/NY-ESO-1157-165-positive multiple myeloma cells. A4 demonstrated antigen-specific binding to HLA-A 0201/NY-ESO-1157-165 redirected T-cells. Expansion with A4 resulted in 98% of HLA-A 0201/NY-ESO-1157-165 redirected T-cells. A4 induced strong proliferation, resulting in a 300-fold increase of redirected T-cells. After expansion protocols, redirected T-cells secreted Interleukin-2, (IL-2), interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα) and lysed target cells in vitro and were protective in vivo. A4 expanded HLA-A 0201/NY-ESO-1157-165 redirected T-cells with preservation of antigen-specific function.

  3. VizieR Online Data Catalog: ESO 37-1 (E 3) VI photometry (de la Fuente Marcos+, 2015)

    Science.gov (United States)

    de La Fuente Marcos, R.; de La Fuente Marcos, C.; Moni Bidin, C.; Ortolani, S.; Carraro, G.

    2015-07-01

    Photometric data in VI for stars in the field of the globular cluster ESO 37-1 (E3). The observations were made on the night of 13 November 2006 with the Very Large Telescope UT2 Kueyen and the FORS1 CCD camera. (1 data file).

  4. Expression of MAGE-A3, NY-ESO-1, LAGE-1 and PRAME in urothelial carcinoma

    DEFF Research Database (Denmark)

    Andersen, Lars Dyrskjøt; Zieger, K; Kissow Lildal, T

    2012-01-01

    Background: The potential for cancer-testis (CT) antigens as targets for immunotherapy in cancer patients has been heavily investigated, and currently cancer vaccine trials based on the CT antigens, MAGE-A3 and NY-ESO-1, are being carried out. Methods: We used specific q-RT-PCR assays to analyse...... the expression of the CT genes MAGE-A3, NY-ESO-1 (CTAG1B), LAGE-1 (CTAG2) and PRAME in a panel of bladder tumours from 350 patients with long-term follow-up and detailed treatment information. Results: Overall, 43% of the tumours expressed MAGE-A3, 35% expressed NY-ESO-1, 27% expressed LAGE-1 and 20% expressed...... PRAME. In all, 56% of the tumours expressed at least one of the CT genes analysed. Univariate Cox regression analysis of CT gene expression in non-muscle-invasive tumours showed that expression of MAGE-A3 (P=0.026), LAGE-1 (P=0.001) and NY-ESO-1 (P=0.040) was significantly associated with a shorter...

  5. Back-stepping active disturbance rejection control design for integrated missile guidance and control system via reduced-order ESO.

    Science.gov (United States)

    Xingling, Shao; Honglun, Wang

    2015-07-01

    This paper proposes a novel composite integrated guidance and control (IGC) law for missile intercepting against unknown maneuvering target with multiple uncertainties and control constraint. First, by using back-stepping technique, the proposed IGC law design is separated into guidance loop and control loop. The unknown target maneuvers and variations of aerodynamics parameters in guidance and control loop are viewed as uncertainties, which are estimated and compensated by designed model-assisted reduced-order extended state observer (ESO). Second, based on the principle of active disturbance rejection control (ADRC), enhanced feedback linearization (FL) based control law is implemented for the IGC model using the estimates generated by reduced-order ESO. In addition, performance analysis and comparisons between ESO and reduced-order ESO are examined. Nonlinear tracking differentiator is employed to construct the derivative of virtual control command in the control loop. Third, the closed-loop stability for the considered system is established. Finally, the effectiveness of the proposed IGC law in enhanced interception performance such as smooth interception course, improved robustness against multiple uncertainties as well as reduced control consumption during initial phase are demonstrated through simulations. Copyright © 2015 ISA. Published by Elsevier Ltd. All rights reserved.

  6. Extinction in the Galaxy from Surface Brightnesses of ESO-LV Galaxies : Determination of A_R/A_B ratio

    NARCIS (Netherlands)

    Choloniewski, J.; Valentijn, E. A.

    A new method for the determination of the extinction in the Galaxy is proposed. The method uses surface brightnesses of external galaxies in the B and R-bands. The observational data have been taken from the ESO-LV galaxy catalog. As a first application of our model we derive the ratio of R-band to

  7. Cancer-testis antigens PRAME and NY-ESO-1 correlate with tumour grade and poor prognosis in myxoid liposarcoma.

    Science.gov (United States)

    Iura, Kunio; Kohashi, Kenichi; Hotokebuchi, Yuka; Ishii, Takeaki; Maekawa, Akira; Yamada, Yuichi; Yamamoto, Hidetaka; Iwamoto, Yukihide; Oda, Yoshinao

    2015-07-01

    Myxoid liposarcoma is the second most common liposarcoma. Although myxoid liposarcoma is relatively chemosensitive and thus a good candidate for chemotherapy, cases with relapsed or metastatic disease still have poor outcome. Here, we performed a gene microarray analysis to compare the gene expression profiles in six clinical myxoid liposarcoma samples and three normal adipose tissue samples, and to identify molecular biomarkers that would be useful as diagnostic markers or treatment targets in myxoid liposarcoma. This showed that the cancer-testis antigen PRAME was up-regulated in myxoid liposarcoma. We then performed immunohistochemical, western blotting and real-time polymerase chain reaction analyses to quantify the expression of PRAME and another cancer-testis antigen, NY-ESO-1, in clinical samples of myxoid liposarcoma (n = 93), dedifferentiated (n = 46), well-differentiated (n = 32) and pleomorphic liposarcomas (n = 14). Immunohistochemically, positivity for PRAME and NY-ESO-1 was observed in 84/93 (90%) and 83/93 (89%) of the myxoid liposarcomas, and in 20/46 (43%) and 3/46 (7%) of the dedifferentiated, 3/32 (9%) and 1/32 (3%) of the well-differentiated and 7/14 (50%) and 3/21 (21%) of the pleomorphic liposarcomas, respectively. High immunohistochemical expression of PRAME and/or NY-ESO-1 was significantly correlated with tumour diameter, the existence of tumour necrosis, a round-cell component of >5%, higher histological grade and advanced clinical stage. High PRAME and NY-ESO-1 expression correlated significantly with poor prognosis in a univariate analysis. The myxoid liposarcomas showed significantly higher protein and mRNA expression levels of PRAME and NY-ESO-1 (CTAG1B) than the other liposarcomas. In conclusion, PRAME and NY-ESO-1 (CTAG1B) were expressed in the vast majority of myxoid liposarcomas, and their high-level expression correlated with tumour grade and poor prognosis. Our results support the potential use of PRAME and NY-ESO

  8. A pilot study of peripheral blood BDCA-1 (CD1c) positive dendritic cells pulsed with NY-ESO-1 ISCOMATRIX™ adjuvant.

    Science.gov (United States)

    Davis, Ian D; Quirk, Juliet; Morris, Leone; Seddon, Lauren; Tai, Tsin Yee; Whitty, Genevieve; Cavicchiolo, Tina; Ebert, Lisa; Jackson, Heather; Browning, Judy; MacGregor, Duncan; Wittke, Frederick; Winkels, Gregor; Alex, Regina; Miloradovic, Lena; Maraskovsky, Eugene; Chen, Weisan; Cebon, Jonathan

    2017-03-01

    Pilot clinical trial of NY-ESO-1 (ESO) protein in ISCOMATRIX™ adjuvant pulsed onto peripheral blood dendritic cells (PBDC), to ascertain feasibility, evaluate toxicity and assess induction of ESO-specific immune responses. Eligible participants had resected cancers expressing ESO or LAGE-1 and were at high risk of relapse. PBDC were produced using CliniMACS ® plus, with initial depletion of CD1c + B cells followed by positive selection of CD1c + PBDC. Patients received three intradermal vaccinations of ESO/IMX-pulsed PBDC at 4-week intervals. The process was feasible and safe. No vaccine-induced immune responses were detected. Assays of immunomodulatory cells did not correlate with outcomes. One patient had a long lasting complete remission. This method was feasible and safe but was minimally immunogenic.

  9. Phase II trial of ipilimumab in melanoma patients with preexisting humoural immune response to NY-ESO-1.

    Science.gov (United States)

    Haag, G M; Zoernig, I; Hassel, J C; Halama, N; Dick, J; Lang, N; Podola, L; Funk, J; Ziegelmeier, C; Juenger, S; Bucur, M; Umansky, L; Falk, C S; Freitag, A; Karapanagiotou-Schenkel, I; Beckhove, P; Enk, A; Jaeger, D

    2018-02-01

    Immune checkpoint therapy has dramatically changed treatment options in patients with metastatic melanoma. However, a relevant part of patients still does not respond to treatment. Data regarding the prognostic or predictive significance of preexisting immune responses against tumour antigens are conflicting. Retrospective data suggested a higher clinical benefit of ipilimumab in melanoma patients with preexisting NY-ESO-1-specific immunity. Twenty-five patients with previously untreated or treated metastatic melanoma and preexisting humoural immune response against NY-ESO-1 received ipilimumab at a dose of 10 mg/kg in week 1, 4, 7, 10 followed by 3-month maintenance treatment for a maximum of 48 weeks. Primary endpoint was the disease control rate (irCR, irPR or irSD) according to immune-related response criteria (irRC). Secondary endpoints included the disease control rate according to RECIST criteria, progression-free survival and overall survival (OS). Humoural and cellular immune responses against NY-ESO-1 were analysed from blood samples. Disease control rate according to irRC was 52%, irPR was observed in 36% of patients. Progression-free survival according to irRC was 7.8 months, according to RECIST criteria it was 2.9 months. Median OS was 22.7 months; the corresponding 1-year survival rate was 66.8%. Treatment-related grade 3 AEs occurred in 36% with no grade 4-5 AEs. No clear association was found between the presence of NY-ESO-1-specific cellular or humoural immune responses and clinical activity. Ipilimumab demonstrated clinically relevant activity within this biomarker-defined population. NY-ESO-1 positivity, as a surrogate for a preexisting immune response against tumour antigens, might help identifying patients with a superior outcome from immune checkpoint blockade. NCT01216696. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. The Gaia-ESO Survey: Exploring the complex nature and origins of the Galactic bulge populations

    Science.gov (United States)

    Rojas-Arriagada, A.; Recio-Blanco, A.; de Laverny, P.; Mikolaitis, Š.; Matteucci, F.; Spitoni, E.; Schultheis, M.; Hayden, M.; Hill, V.; Zoccali, M.; Minniti, D.; Gonzalez, O. A.; Gilmore, G.; Randich, S.; Feltzing, S.; Alfaro, E. J.; Babusiaux, C.; Bensby, T.; Bragaglia, A.; Flaccomio, E.; Koposov, S. E.; Pancino, E.; Bayo, A.; Carraro, G.; Casey, A. R.; Costado, M. T.; Damiani, F.; Donati, P.; Franciosini, E.; Hourihane, A.; Jofré, P.; Lardo, C.; Lewis, J.; Lind, K.; Magrini, L.; Morbidelli, L.; Sacco, G. G.; Worley, C. C.; Zaggia, S.

    2017-05-01

    Context. As observational evidence steadily accumulates, the nature of the Galactic bulge has proven to be rather complex: the structural, kinematic, and chemical analyses often lead to contradictory conclusions. The nature of the metal-rich bulge - and especially of the metal-poor bulge - and their relation with other Galactic components, still need to be firmly defined on the basis of statistically significant high-quality data samples. Aims: We used the fourth internal data release of the Gaia-ESO survey to characterize the bulge metallicity distribution function (MDF), magnesium abundance, spatial distribution, and correlation of these properties with kinematics. Moreover, the homogeneous sampling of the different Galactic populations provided by the Gaia-ESO survey allowed us to perform a comparison between the bulge, thin disk, and thick disk sequences in the [Mg/Fe] vs. [Fe/H] plane in order to constrain the extent of their eventual chemical similarities. Methods: We obtained spectroscopic data for 2500 red clump stars in 11 bulge fields, sampling the area -10° ≤ l ≤ + 8° and -10° ≤ b ≤ -4° from the fourth internal data release of the Gaia-ESO survey. A sample of 6300 disk stars was also selected for comparison. Spectrophotometric distances computed via isochrone fitting allowed us to define a sample of stars likely located in the bulge region. Results: From a Gaussian mixture models (GMM) analysis, the bulge MDF is confirmed to be bimodal across the whole sampled area. The relative ratio between the two modes of the MDF changes as a function of b, with metal-poor stars dominating at high latitudes. The metal-rich stars exhibit bar-like kinematics and display a bimodality in their magnitude distribution, a feature which is tightly associated with the X-shape bulge. They overlap with the metal-rich end of the thin disk sequence in the [Mg/Fe] vs. [Fe/H] plane. On the other hand, metal-poor bulge stars have a more isotropic hot kinematics and do

  11. Comprehensive adipocytic and neurogenic tissue microarray analysis of NY-ESO-1 expression - a promising immunotherapy target in malignant peripheral nerve sheath tumor and liposarcoma.

    Science.gov (United States)

    Shurell, Elizabeth; Vergara-Lluri, Maria E; Li, Yunfeng; Crompton, Joseph G; Singh, Arun; Bernthal, Nicholas; Wu, Hong; Eilber, Fritz C; Dry, Sarah M

    2016-11-08

    Immunotherapy targeting cancer-testis antigen NY-ESO-1 shows promise for tumors with poor response to chemoradiation. Malignant peripheral nerve sheath tumors (MPNSTs) and liposarcomas (LPS) are chemoresistant and have few effective treatment options. Materials Methods: Using a comprehensive tissue microarray (TMA) of both benign and malignant tumors in primary, recurrent, and metastatic samples, we examined NY-ESO-1 expression in peripheral nerve sheath tumor (PNST) and adipocytic tumors. The PNST TMA included 42 MPNSTs (spontaneous n = 26, NF1-associated n = 16), 35 neurofibromas (spontaneous n = 22, NF-1 associated n = 13), 11 schwannomas, and 18 normal nerves. The LPS TMA included 48 well-differentiated/dedifferentiated (WD/DD) LPS, 13 myxoid/round cell LPS, 3 pleomorphic LPS, 8 lipomas, 1 myelolipoma, and 3 normal adipocytic tissue samples. Stained in triplicate, NY-ESO-1 intensity and density were scored. NY-ESO-1 expression was exclusive to malignant tumors. 100% of myxoid/round cell LPS demonstrated NY-ESO-1 expression, while only 6% of WD/DD LPS showed protein expression, one of which was WD LPS. Of MPNST, 4/26 (15%) spontaneous and 2/16 (12%) NF1-associated MPNSTs demonstrated NY-ESO-1 expression. Strong NY-ESO-1 expression was observed in myxoid/round cell and dedifferentiated LPS, and MPNST in primary, neoadjuvant, and metastatic settings. We found higher prevalence of NY-ESO-1 expression in MPNSTs than previously reported, highlighting a subset of MPNST patients who may benefit from immunotherapy. This study expands our understanding of NY-ESO-1 in WD/DD LPS and is the first demonstration of staining in a WD LPS and metastatic/recurrent myxoid/round cell LPS. These results suggest immunotherapy targeting NY-ESO-1 may benefit patients with aggressive tumors resistant to conventional therapy.

  12. Treatment with 5-Aza-2'-Deoxycytidine Induces Expression of NY-ESO-1 and Facilitates Cytotoxic T Lymphocyte-Mediated Tumor Cell Killing.

    Science.gov (United States)

    Klar, Agnes S; Gopinadh, Jakka; Kleber, Sascha; Wadle, Andreas; Renner, Christoph

    2015-01-01

    NY-ESO-1 belongs to the cancer/testis antigen (CTA) family and represents an attractive target for cancer immunotherapy. Its expression is induced in a variety of solid tumors via DNA demethylation of the promoter of CpG islands. However, NY-ESO-1 expression is usually very low or absent in some tumors such as breast cancer or multiple myeloma. Therefore, we established an optimized in vitro treatment protocol for up-regulation of NY-ESO-1 expression by tumor cells using the hypomethylating agent 5-aza-2'-deoxycytidine (DAC). We demonstrated de novo induction of NY-ESO-1 in MCF7 breast cancer cells and significantly increased expression in U266 multiple myeloma cells. This effect was time- and dose-dependent with the highest expression of NY-ESO-1 mRNA achieved by the incubation of 10 μM DAC for 72 hours. NY-ESO-1 activation was also confirmed at the protein level as shown by Western blot, flow cytometry, and immunofluorescence staining. The detection and quantification of single NY-ESO-1 peptides presented at the tumor cell surface in the context of HLA-A*0201 molecules revealed an increase of 100% and 50% for MCF7 and U266 cells, respectively. Moreover, the enhanced expression of NY-ESO-1 derived peptides at the cell surface was accompanied by an increased specific lysis of MCF7 and U266 cells by HLA-A*0201/NY-ESO-1(157-165) peptide specific chimeric antigen receptor (CAR) CD8+ T cells. In addition, the killing activity of CAR T cells correlated with the secretion of higher IFN-gamma levels. These results indicate that NY-ESO-1 directed immunotherapy with specific CAR T cells might benefit from concomitant DAC treatment.

  13. Treatment with 5-Aza-2'-Deoxycytidine Induces Expression of NY-ESO-1 and Facilitates Cytotoxic T Lymphocyte-Mediated Tumor Cell Killing.

    Directory of Open Access Journals (Sweden)

    Agnes S Klar

    Full Text Available NY-ESO-1 belongs to the cancer/testis antigen (CTA family and represents an attractive target for cancer immunotherapy. Its expression is induced in a variety of solid tumors via DNA demethylation of the promoter of CpG islands. However, NY-ESO-1 expression is usually very low or absent in some tumors such as breast cancer or multiple myeloma. Therefore, we established an optimized in vitro treatment protocol for up-regulation of NY-ESO-1 expression by tumor cells using the hypomethylating agent 5-aza-2'-deoxycytidine (DAC.We demonstrated de novo induction of NY-ESO-1 in MCF7 breast cancer cells and significantly increased expression in U266 multiple myeloma cells. This effect was time- and dose-dependent with the highest expression of NY-ESO-1 mRNA achieved by the incubation of 10 μM DAC for 72 hours. NY-ESO-1 activation was also confirmed at the protein level as shown by Western blot, flow cytometry, and immunofluorescence staining. The detection and quantification of single NY-ESO-1 peptides presented at the tumor cell surface in the context of HLA-A*0201 molecules revealed an increase of 100% and 50% for MCF7 and U266 cells, respectively. Moreover, the enhanced expression of NY-ESO-1 derived peptides at the cell surface was accompanied by an increased specific lysis of MCF7 and U266 cells by HLA-A*0201/NY-ESO-1(157-165 peptide specific chimeric antigen receptor (CAR CD8+ T cells. In addition, the killing activity of CAR T cells correlated with the secretion of higher IFN-gamma levels.These results indicate that NY-ESO-1 directed immunotherapy with specific CAR T cells might benefit from concomitant DAC treatment.

  14. The Gaia-ESO Survey: double-, triple-, and quadruple-line spectroscopic binary candidates

    Science.gov (United States)

    Merle, T.; Van Eck, S.; Jorissen, A.; Van der Swaelmen, M.; Masseron, T.; Zwitter, T.; Hatzidimitriou, D.; Klutsch, A.; Pourbaix, D.; Blomme, R.; Worley, C. C.; Sacco, G.; Lewis, J.; Abia, C.; Traven, G.; Sordo, R.; Bragaglia, A.; Smiljanic, R.; Pancino, E.; Damiani, F.; Hourihane, A.; Gilmore, G.; Randich, S.; Koposov, S.; Casey, A.; Morbidelli, L.; Franciosini, E.; Magrini, L.; Jofre, P.; Costado, M. T.; Jeffries, R. D.; Bergemann, M.; Lanzafame, A. C.; Bayo, A.; Carraro, G.; Flaccomio, E.; Monaco, L.; Zaggia, S.

    2017-12-01

    Context. The Gaia-ESO Survey (GES) is a large spectroscopic survey that provides a unique opportunity to study the distribution of spectroscopic multiple systems among different populations of the Galaxy. Aims: Our aim is to detect binarity/multiplicity for stars targeted by the GES from the analysis of the cross-correlation functions (CCFs) of the GES spectra with spectral templates. Methods: We developed a method based on the computation of the CCF successive derivatives to detect multiple peaks and determine their radial velocities, even when the peaks are strongly blended. The parameters of the detection of extrema (DOE) code have been optimized for each GES GIRAFFE and UVES setup to maximize detection. The DOE code therefore allows to automatically detect multiple line spectroscopic binaries (SBn, n ≥ 2). Results: We apply this method on the fourth GES internal data release and detect 354 SBn candidates (342 SB2, 11 SB3, and even one SB4), including only nine SBs known in the literature. This implies that about 98% of these SBn candidates are new because of their faint visual magnitude that can reach V = 19. Visual inspection of the SBn candidate spectra reveals that the most probable candidates have indeed a composite spectrum. Among the SB2 candidates, an orbital solution could be computed for two previously unknown binaries: CNAME 06404608+0949173 (known as V642 Mon) in NGC 2264 and CNAME 19013257-0027338 in Berkeley 81 (Be 81). A detailed analysis of the unique SB4 (four peaks in the CCF) reveals that CNAME 08414659-5303449 (HD 74438) in the open cluster IC 2391 is a physically bound stellar quadruple system. The SB candidates belonging to stellar clusters are reviewed in detail to discard false detections. We suggest that atmospheric parameters should not be used for these system components; SB-specific pipelines should be used instead. Conclusions: Our implementation of an automatic detection of spectroscopic binaries within the GES has allowed the

  15. Gaia-ESO Survey: Analysis of pre-main sequence stellar spectra

    Science.gov (United States)

    Lanzafame, A. C.; Frasca, A.; Damiani, F.; Franciosini, E.; Cottaar, M.; Sousa, S. G.; Tabernero, H. M.; Klutsch, A.; Spina, L.; Biazzo, K.; Prisinzano, L.; Sacco, G. G.; Randich, S.; Brugaletta, E.; Delgado Mena, E.; Adibekyan, V.; Montes, D.; Bonito, R.; Gameiro, J. F.; Alcalá, J. M.; González Hernández, J. I.; Jeffries, R.; Messina, S.; Meyer, M.; Gilmore, G.; Asplund, M.; Binney, J.; Bonifacio, P.; Drew, J. E.; Feltzing, S.; Ferguson, A. M. N.; Micela, G.; Negueruela, I.; Prusti, T.; Rix, H.-W.; Vallenari, A.; Alfaro, E. J.; Allende Prieto, C.; Babusiaux, C.; Bensby, T.; Blomme, R.; Bragaglia, A.; Flaccomio, E.; Francois, P.; Hambly, N.; Irwin, M.; Koposov, S. E.; Korn, A. J.; Smiljanic, R.; Van Eck, S.; Walton, N.; Bayo, A.; Bergemann, M.; Carraro, G.; Costado, M. T.; Edvardsson, B.; Heiter, U.; Hill, V.; Hourihane, A.; Jackson, R. J.; Jofré, P.; Lardo, C.; Lewis, J.; Lind, K.; Magrini, L.; Marconi, G.; Martayan, C.; Masseron, T.; Monaco, L.; Morbidelli, L.; Sbordone, L.; Worley, C. C.; Zaggia, S.

    2015-04-01

    Context. The Gaia-ESO Public Spectroscopic Survey is obtaining high-quality spectroscopy of some 100 000 Milky Way stars using the FLAMES spectrograph at the VLT, down to V = 19 mag, systematically covering all the main components of the Milky Way and providing the first homogeneous overview of the distributions of kinematics and chemical element abundances in the Galaxy. Observations of young open clusters, in particular, are giving new insights into their initial structure, kinematics, and their subsequent evolution. Aims: This paper describes the analysis of UVES and GIRAFFE spectra acquired in the fields of young clusters whose population includes pre-main sequence (PMS) stars. The analysis is applied to all stars in such fields, regardless of any prior information on membership, and provides fundamental stellar atmospheric parameters, elemental abundances, and PMS-specific parameters such as veiling, accretion, and chromospheric activity. Methods: When feasible, different methods were used to derive raw parameters (e.g. line equivalent widths) fundamental atmospheric parameters and derived parameters (e.g. abundances). To derive some of these parameters, we used methods that have been extensively used in the past and new ones developed in the context of the Gaia-ESO survey enterprise. The internal precision of these quantities was estimated by inter-comparing the results obtained by these different methods, while the accuracy was estimated by comparison with independent external data, such as effective temperature and surface gravity derived from angular diameter measurements, on a sample of benchmarks stars. A validation procedure based on these comparisons was applied to discard spurious or doubtful results and produce recommended parameters. Specific strategies were implemented to resolve problems of fast rotation, accretion signatures, chromospheric activity, and veiling. Results: The analysis carried out on spectra acquired in young cluster fields during

  16. The Gaia-ESO survey: the inner disk intermediate-age open cluster NGC 6802

    Science.gov (United States)

    Tang, B.; Geisler, D.; Friel, E.; Villanova, S.; Smiljanic, R.; Casey, A. R.; Randich, S.; Magrini, L.; San Roman, I.; Muñoz, C.; Cohen, R. E.; Mauro, F.; Bragaglia, A.; Donati, P.; Tautvaišien*error*ė, G.; Drazdauskas, A.; Ženovienė, R.; Snaith, O.; Sousa, S.; Adibekyan, V.; Costado, M. T.; Blanco-Cuaresma, S.; Jiménez-Esteban, F.; Carraro, G.; Zwitter, T.; François, P.; Jofrè, P.; Sordo, R.; Gilmore, G.; Flaccomio, E.; Koposov, S.; Korn, A. J.; Lanzafame, A. C.; Pancino, E.; Bayo, A.; Damiani, F.; Franciosini, E.; Hourihane, A.; Lardo, C.; Lewis, J.; Monaco, L.; Morbidelli, L.; Prisinzano, L.; Sacco, G.; Worley, C. C.; Zaggia, S.

    2017-05-01

    Milky Way open clusters are very diverse in terms of age, chemical composition, and kinematic properties. Intermediate-age and old open clusters are less common, and it is even harder to find them inside the solar Galactocentric radius, due to the high mortality rate and strong extinction inside this region. NGC 6802 is one of the inner disk open clusters (IOCs) observed by the Gaia-ESO survey (GES). This cluster is an important target for calibrating the abundances derived in the survey due to the kinematic and chemical homogeneity of the members in open clusters. Using the measurements from Gaia-ESO internal data release 4 (iDR4), we identify 95 main-sequence dwarfs as cluster members from the GIRAFFE target list, and eight giants as cluster members from the UVES target list. The dwarf cluster members have a median radial velocity of 13.6 ± 1.9 km s-1, while the giant cluster members have a median radial velocity of 12.0 ± 0.9 km s-1 and a median [Fe/H] of 0.10 ± 0.02 dex. The color-magnitude diagram of these cluster members suggests an age of 0.9 ± 0.1 Gyr, with (m-M)0 = 11.4 and E(B-V) = 0.86. We perform the first detailed chemical abundance analysis of NGC 6802, including 27 elemental species. To gain a more general picture about IOCs, the measurements of NGC 6802 are compared with those of other IOCs previously studied by GES, that is, NGC 4815, Trumpler 20, NGC 6705, and Berkeley 81. NGC 6802 shows similar C, N, Na, and Al abundances as other IOCs. These elements are compared with nucleosynthetic models as a function of cluster turn-off mass. The α, iron-peak, and neutron-capture elements are also explored in a self-consistent way. Full Tables A.3-A.6 are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/601/A56

  17. Immunoediting and persistence of antigen-specific immunity in patients who have previously been vaccinated with NY-ESO-1 protein formulated in ISCOMATRIX™.

    Science.gov (United States)

    Nicholaou, Theo; Chen, Weisan; Davis, Ian D; Jackson, Heather M; Dimopoulos, Nektaria; Barrow, Catherine; Browning, Judy; Macgregor, Duncan; Williams, David; Hopkins, Wendie; Maraskovsky, Eugene; Venhaus, Ralph; Pan, Linda; Hoffman, Eric W; Old, Lloyd J; Cebon, Jonathan

    2011-11-01

    NY-ESO-1 protein formulated in ISCOMATRIX™ results in CD4+, CD8+ T cell and antibody-mediated immunity. We evaluated persistence of immunity, relapse-free survival and tumour antigen expression upon relapse in patients vaccinated in an earlier trial. Immunity was measured in 28 patients with resected NY-ESO-1-expressing tumours (melanoma 25, breast 3) 252-1,155 days (median = 681) after vaccination. In the earlier vaccination, trial patients received NY-ESO-1 with ISCOMATRIX™ adjuvant at three protein doses 10 μg, 30 μg or 100 μg (n = 14); 100 μg NY-ESO-1 protein (n = 8) or placebo (n = 6), together with 1 μg of intradermal (ID) NY-ESO-1 protein twice for DTH skin testing. Immune responses assessed in the current study included antibody titres, circulating NY-ESO-1-specific T cells and DTH reactivity 2 days after DTH skin testing with NY-ESO-1 protein (1 μg) or peptides (10 μg). Relapse-free survival was determined for 42 melanoma patients. On relapse NY-ESO-1 and HLA, class I was assessed by immunohistochemistry in 17. Persisting anti-NY-ESO-1 immunity was detected in 10/14 recipients who had previously received vaccine with ISCOMATRIX™ adjuvant. In contrast, immunity only persisted in 3/14 who received 100 μg un-adjuvanted NY-ESO-1 protein (3/8) or 2 μg DTH protein (0/6) P = 0.02. Hence, persisting NY-ESO-1 immunity was associated with prior adjuvant. Tumour NY-ESO-1 or HLA class I was downregulated in participants who relapsed suggesting immunoediting had occurred. Immunoediting suggests that a signal of anti-tumour activity was observed in high-risk resected melanoma patients vaccinated with NY-ESO-1/ISCOMATRIX™. This was associated with measurable persisting immunity in the majority of vaccinated subjects tested. A prospective randomised trial has been undertaken to confirm these results.

  18. The cancer-testis antigen NY-ESO-1 is highly expressed in myxoid and round cell subset of liposarcomas.

    Science.gov (United States)

    Hemminger, Jessica A; Ewart Toland, Amanda; Scharschmidt, Thomas J; Mayerson, Joel L; Kraybill, William G; Guttridge, Denis C; Iwenofu, O Hans

    2013-02-01

    Liposarcomas are a heterogenous group of fat-derived sarcomas, and surgery with or without chemoradiation therapy remains the main stay of treatment. NY-ESO-1 is a cancer-testis antigen expressed in various cancers where it can induce both cellular and humoral immunity. Immunotherapy has shown promise in clinical trials involving NY-ESO-1-expressing tumors. Gene expression studies have shown upregulation of the gene for NY-ESO-1, CTAG1B, in myxoid and round cell liposarcomas. Herein, we evaluated the expression of NY-ESO-1 among liposarcoma subtypes by quantitative real-time PCR, western blot analysis, and immunohistochemistry. Frozen tissue for quantitative real-time PCR and western blot analysis was obtained for the following liposarcoma subtypes (n=15): myxoid and round cell (n=8); well-differentiated (n=4), and dedifferentiated (n=3). Formalin-fixed paraffin-embedded blocks were obtained for the following liposarcoma subtypes (n=44): myxoid and round cell (n=18); well-differentiated (n=10); dedifferentiated (n=10); and pleomorphic (n=6). Full sections were stained with monoclonal antibody NY-ESO-1, and staining was assessed for intensity (1-3+), percentage of tumor positivity, and location. In all, 7/8 (88%) and 16/18 (89%) myxoid and round cell expressed CTAG1B and NY-ESO-1 by quantitative real-time PCR and immunohistochemistry, respectively. Western blot correlated with mRNA expression levels. By immunohistochemistry, 94% (15/16) of positive cases stained homogenously with 2-3+ intensity. Also, 3/6 (50%) pleomorphic liposarcomas demonstrated a range of staining: 1+ intensity in 50% of cells; 2+ intensity in 5% of cells; and 3+ intensity in 90% of cells. One case of dedifferentiated liposarcoma showed strong, diffuse staining (3+ intensity in 75% of cells). Our study shows that both CTAG1B mRNA and protein are overexpressed with high frequency in myxoid and round cell liposarcoma, enabling the potential use of targeted immunotherapy in the treatment of this

  19. THE NARROW X-RAY TAIL AND DOUBLE Hα TAILS OF ESO 137-002 IN A3627

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, B.; Lin, X. B.; Kong, X. [Center for Astrophysics, University of Science and Technology of China, Hefei, Anhui 230026 (China); Sun, M. [Department of Physics, University of Alabama in Huntsville, Huntsville, AL 35899 (United States); Ji, L. [Purple Mountain Observatory, Chinese Academy of Sciences, Nanjing, Jiangsu 210008 (China); Sarazin, C. [Department of Astronomy, University of Virginia, P.O. Box 400325, Charlottesville, VA 22904-4325 (United States); Nulsen, P. E. J.; Forman, W.; Jones, C. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Roediger, E. [Germany Hamburger Sternwarte, Universität Hamburg, Gojensbergsweg 112, D-21029 Hamburg (Germany); Donahue, M.; Voit, G. M., E-mail: xkong@ustc.edu.cn, E-mail: mingsun.cluster@gmail.com [Department of Physics and Astronomy, Michigan State University, East Lansing, MI 48824 (United States)

    2013-11-10

    We present the analysis of a deep Chandra observation of a ∼2 L{sub *} late-type galaxy, ESO 137-002, in the closest rich cluster A3627. The Chandra data reveal a long (∼>40 kpc) and narrow tail with a nearly constant width (∼3 kpc) to the southeast of the galaxy, and a leading edge ∼1.5 kpc from the galaxy center on the upstream side of the tail. The tail is most likely caused by the nearly edge-on stripping of ESO 137-002's interstellar medium (ISM) by ram pressure, compared to the nearly face-on stripping of ESO 137-001 discussed in our previous work. Spectral analysis of individual regions along the tail shows that the gas throughout it has a rather constant temperature, ∼1 keV, very close to the temperature of the tails of ESO 137-001, if the same atomic database is used. The derived gas abundance is low (∼0.2 solar with the single-kT model), an indication of the multiphase nature of the gas in the tail. The mass of the X-ray tail is only a small fraction (<5%) of the initial ISM mass of the galaxy, suggesting that the stripping is most likely at an early stage. However, with any of the single-kT, double-kT, and multi-kT models we tried, the tail is always 'over-pressured' relative to the surrounding intracluster medium (ICM), which could be due to the uncertainties in the abundance, thermal versus non-thermal X-ray emission, or magnetic support in the ICM. The Hα data from the Southern Observatory for Astrophysical Research show a ∼21 kpc tail spatially coincident with the X-ray tail, as well as a secondary tail (∼12 kpc long) to the east of the main tail diverging at an angle of ∼23° and starting at a distance of ∼7.5 kpc from the nucleus. At the position of the secondary Hα tail, the X-ray emission is also enhanced at the ∼2σ level. We compare the tails of ESO 137-001 and ESO 137-002, and also compare the tails to simulations. Both the similarities and differences of the tails pose challenges to the simulations

  20. SUZAKU OBSERVATIONS OF THE X-RAY BRIGHTEST FOSSIL GROUP ESO 3060170

    International Nuclear Information System (INIS)

    Su, Yuanyuan; White, Raymond E. III; Miller, Eric D.

    2013-01-01

    'Fossil' galaxy groups, each dominated by a relatively isolated giant elliptical galaxy, have many properties intermediate between groups and clusters of galaxies. We used the Suzaku X-ray observatory to observe the X-ray brightest fossil group, ESO 3060170, out to R 200 , in order to better elucidate the relation between fossil groups, normal groups, and clusters. We determined the intragroup gas temperature, density, and metal abundance distributions and derived the entropy, pressure, and mass profiles for this group. The entropy and pressure profiles in the outer regions are flatter than in simulated clusters, similar to what is seen in observations of massive clusters. This may indicate that the gas is clumpy and/or the gas has been redistributed. Assuming hydrostatic equilibrium, the total mass is estimated to be ∼1.7 × 10 14 M ☉ within a radius R 200 of ∼1.15 Mpc, with an enclosed baryon mass fraction of 0.13. The integrated iron mass-to-light ratio of this fossil group is larger than in most groups and comparable to those of clusters, indicating that this fossil group has retained the bulk of its metals. A galaxy luminosity density map on a scale of 25 Mpc shows that this fossil group resides in a relatively isolated environment, unlike the filamentary structures in which typical groups and clusters are embedded

  1. CRIRES+ : A Cross-dispersed High-resolution Infrared Spectrograph for ESO's VLT

    Science.gov (United States)

    Hatzes, Artie; CRIRES+ Team

    2017-06-01

    CRIRES+ is a major upgrade to the former CRyogenic high resolution Infra-Red Echelle Spectrograph of ESO's 8.2m Very Large Telescope. The major science drivers for this upgrade are the confirmation and characterization (e.g. determination of the mass) of rocky planets in the so-called habitable zone of M-dwarf stars via radial velocity measurements, the characterization of exoplanet atmospheres, and the study of magnetic fields in low mass stars and brown dwarfs. CRIRES+ will maintain the high resolving power (R = 100,000) of its predecessor in the Y, J, H, K, L and M bands, but it will include the following improvements: 1) CRIRES+ will be cross-dispersed recording 8-9 diffraction orders at a time, increasing the observing efficiency approximately by an order of magnitude. 2) New detectors with better sensitivity and cosmetics over the old devices. 3) A new gas absorption cell for improved wavelength calibration. This along with the increased wavelength coverage should yield a radial velocity measurement precision to better than 2-5 m/s in K-band. In addition, in Y to K bands, a new Fabry-Perot etalon device will ensure a precision of 100 m/s. 4) A polarimetric unit which will measure both circular and linear polarization. We present the current status and schedule of the project. The instrument is currently scheduled to be installed at the telescope beginning 2018.

  2. Final binary star results from the ESO VLT Lunar occultations program

    Energy Technology Data Exchange (ETDEWEB)

    Richichi, A. [National Astronomical Research Institute of Thailand, 191 Siriphanich Bldg., Huay Kaew Road, Suthep, Muang, Chiang Mai 50200 (Thailand); Fors, O. [Departament Astronomia i Meteorologia and Institut de Ciències del Cosmos (ICC), Universitat de Barcelona (UB/IEEC), Martí i Franqués 1, E-08028 Barcelona (Spain); Cusano, F. [INAF-Osservatorio Astronomico di Bologna, Via Ranzani 1, I-40127 Bologna (Italy); Ivanov, V. D., E-mail: andrea4work@gmail.com [European Southern Observatory, Ave. Alonso de Cordova 3107, Casilla 19001, Santiago 19 (Chile)

    2014-03-01

    We report on 13 subarcsecond binaries, detected by means of lunar occultations in the near-infrared at the ESO Very Large Telescope (VLT). They are all first-time detections except for the visual binary HD 158122, which we resolved for the first time in the near-infrared. The primaries have magnitudes in the range K = 4.5-10.0, and companions in the range K = 6.8-11.1. The magnitude differences have a median value of 2.4, with the largest being 4.6. The projected separations are in the range of 4-168 mas, with a median of 13 mas. We discuss and compare our results with the available literature. With this paper, we conclude the mining for binary star detections in the 1226 occultations recorded at the VLT with the ISAAC instrument. We expect that the majority of these binaries may be unresolvable by adaptive optics on current telescopes, and they might be challenging for long-baseline interferometry. However, they constitute an interesting sample for future larger telescopes and for astrometric missions such as GAIA.

  3. A psf-fitting pipeline for VVV-ESO: The star cluster Pismis 24

    Science.gov (United States)

    Dias, R. A. G.; Bonatto, C.

    2014-10-01

    Este trabalho apresenta um algoritmo para a extração de dados fotométricos do catálogo "VISTA Variables in the Via Láctea" (VVV) do ESO. A principal característica do algoritmo é evitar a interação com o usuário mantendo precisão e profundidade fotométrica, de fato o algoritmo foi capaz de gerar dados mais precisos para as estrelas menos brilhantes (J gtrsim 16, H gtrsim 15,5 e H gtrsim 15) e confiável fotometria para estrelas mais de uma magnitude mais fracas do que as detectáveis com outras técnicas. Embora o algoritmo obtenha resultados menos precisos para as estrelas mais brilhantes, este provou ser o método mais adequado, uma vez que queremos trabalhar com aglomerados abertos jovens, onde a pré-sequência principal é de maior importância. Além disso, podemos combinar 2MASS e VVV para substituir as estrelas saturadas do VVV.

  4. Fine-mapping naturally occurring NY-ESO-1 antibody epitopes in melanoma patients' sera using short overlapping peptides and full-length recombinant protein.

    Science.gov (United States)

    Komatsu, Nobukazu; Jackson, Heather M; Chan, Kok-fei; Oveissi, Sara; Cebon, Jonathan; Itoh, Kyogo; Chen, Weisan

    2013-07-01

    The tumor antigen NY-ESO-1 is one of the most antigenic cancer-testis antigens, first identified by serologic analysis of a recombinant cDNA expression library (SEREX). NY-ESO-1 is expressed in different types of cancers including melanoma. NY-ESO-1-specific spontaneous humoral and cellular immune responses are detected in a large proportion of patients with advanced NY-ESO-1-expressing cancers. Therefore NY-ESO-1 is a good candidate antigen for immunotherapy. Although cellular immune responses to NY-ESO-1 are well characterized, much less is known about the humoral immune responses. In this study, we finely mapped linear antibody epitopes using sera from melanoma patients and shorter overlapping peptide sets. We have shown that melanoma patients' humoral immune systems responded to NY-ESO-1 differently in each individual with widely differing antibody specificity, intensity and antibody subtypes. This knowledge will help us further understand anti-tumor immunity and may also help us to monitor cancer progress and cancer vaccine efficacy in the future. Copyright © 2013 Elsevier Ltd. All rights reserved.

  5. NY-ESO-1 Vaccination in Combination with Decitabine Induces Antigen-Specific T-lymphocyte Responses in Patients with Myelodysplastic Syndrome.

    Science.gov (United States)

    Griffiths, Elizabeth A; Srivastava, Pragya; Matsuzaki, Junko; Brumberger, Zachary; Wang, Eunice S; Kocent, Justin; Miller, Austin; Roloff, Gregory W; Wong, Hong Yuen; Paluch, Benjamin E; Lutgen-Dunckley, Linda G; Martens, Brandon L; Odunsi, Kunle; Karpf, Adam R; Hourigan, Christopher S; Nemeth, Michael J

    2017-09-25

    Purpose: Treatment options are limited for patients with high-risk myelodysplastic syndrome (MDS). The azanucleosides, azacitidine and decitabine, are first-line therapy for MDS that induce promoter demethylation and gene expression of the highly immunogenic tumor antigen NY-ESO-1. We demonstrated that patients with acute myeloid leukemia (AML) receiving decitabine exhibit induction of NY-ESO-1 expression in circulating blasts. We hypothesized that vaccinating against NY-ESO-1 in patients with MDS receiving decitabine would capitalize upon induced NY-ESO-1 expression in malignant myeloid cells to provoke an NY-ESO-1-specific MDS-directed cytotoxic T-cell immune response. Experimental Design: In a phase I study, 9 patients with MDS received an HLA-unrestricted NY-ESO-1 vaccine (CDX-1401 + poly-ICLC) in a nonoverlapping schedule every four weeks with standard-dose decitabine. Results: Analysis of samples serially obtained from the 7 patients who reached the end of the study demonstrated induction of NY-ESO-1 expression in 7 of 7 patients and NY-ESO-1-specific CD4 + and CD8 + T-lymphocyte responses in 6 of 7 and 4 of 7 of the vaccinated patients, respectively. Myeloid cells expressing NY-ESO-1, isolated from a patient at different time points during decitabine therapy, were capable of activating a cytotoxic response from autologous NY-ESO-1-specific T lymphocytes. Vaccine responses were associated with a detectable population of CD141 Hi conventional dendritic cells, which are critical for the uptake of NY-ESO-1 vaccine and have a recognized role in antitumor immune responses. Conclusions: These data indicate that vaccination against induced NY-ESO-1 expression can produce an antigen-specific immune response in a relatively nonimmunogenic myeloid cancer and highlight the potential for induced antigen-directed immunotherapy in a group of patients with limited options. Clin Cancer Res; 24(5); 1-11. ©2017 AACR. See related commentary by Fuchs, p. 991 . ©2017 American

  6. Propuesta para incluir el patrimonio industrial de Gipuzkoa en los programas de contenidos del área de Tecnología en la ESO

    OpenAIRE

    Soto-Imaz, Maider

    2013-01-01

    Este TFM expone la preocupación sobre la formación en Patrimonio Industrial entre los contenidos del área de Tecnología de la ESO. Concretamente la investigación parte de la situación actual del área en los centros escolares de Gipuzkoa, y continua y completa la visión argumentando la importancia de incluir el enfoque tecnológico del patrimonio industrial local en la formación de los alumnos de la ESO. Se ha utilizado un cuestionario dirigido a los docentes de Tecnología de la ESO de ce...

  7. Rapid generation of NY-ESO-1-specific CD4+ THELPER1 cells for adoptive T-cell therapy

    Science.gov (United States)

    Kayser, Simone; Boβ, Cristina; Feucht, Judith; Witte, Kai-Erik; Scheu, Alexander; Bülow, Hans-Jörg; Joachim, Stefanie; Stevanović, Stefan; Schumm, Michael; Rittig, Susanne M; Lang, Peter; Röcken, Martin; Handgretinger, Rupert; Feuchtinger, Tobias

    2015-01-01

    Tumor-associated antigens such as NY-ESO-1 are expressed in a variety of solid tumors but absent in mature healthy tissues with the exception of germline cells. The immune system anti-cancer attack is mediated by cell lysis or induction of growth arrest through paralysis of tumor cells, the latter of which can be achieved by tumor-specific CD4+, IFNγ-producing THelper type 1 (TH1) cells. Translation of these immune-mediated mechanisms into clinical application has been limited by availability of immune effectors, as well as the need for complex in vitro protocols and regulatory hurdles. Here, we report a procedure to generate cancer-testis antigen NY-ESO-1-targeting CD4+ TH1 cells in vitro for cancer immunotherapy in the clinic. After in vitro sensitization by stimulating T cells with protein-spanning, overlapping peptide pools of NY-ESO-1 in combination with IL-7 and low dose IL-2, antigen-specific T cells were isolated using IFNγ capture technique and subsequently expanded with IL-2, IL-7 and IL-15. Large numbers of NY-ESO-1-specific CD4+ T cells with a TH1 cytokine profile and lower numbers of cytokine-secreting CD8+ T cells could be generated from healthy donors with a high specificity and expansion potential. Manufactured CD4+ T cells showed strong specific TH1-responses with IFNγ+, TNFα+, IL-2+ and induced cell cycle arrest and apoptosis in tumor cells. The protocol is GMP-grade and approved by the regulatory authorities. The tumor-antigen specific CD4+ TH1 lymphocytes can be adoptively transferred as a T-cell therapy to boost anticancer immunity and this novel cancer treatment approach is applicable to both T cells from healthy allogeneic donors as well as to autologous T cells derived from cancer patients. PMID:26155389

  8. The Gaia-ESO Survey: New constraints on the Galactic disc velocity dispersion and its chemical dependencies

    Science.gov (United States)

    Guiglion, G.; Recio-Blanco, A.; de Laverny, P.; Kordopatis, G.; Hill, V.; Mikolaitis, Š.; Minchev, I.; Chiappini, C.; Wyse, R. F. G.; Gilmore, G.; Randich, S.; Feltzing, S.; Bensby, T.; Flaccomio, E.; Koposov, S. E.; Pancino, E.; Bayo, A.; Costado, M. T.; Franciosini, E.; Hourihane, A.; Jofré, P.; Lardo, C.; Lewis, J.; Lind, K.; Magrini, L.; Morbidelli, L.; Sacco, G. G.; Ruchti, G.; Worley, C. C.; Zaggia, S.

    2015-11-01

    Context. Understanding the history and the evolution of the Milky Way is one of the main goals of modern astrophysics. In particular, the formation of the Galactic disc is a key problem of Galactic archaeology. Aims: We study the velocity dispersion behaviour of Galactic disc stars as a function of the [Mg/Fe] ratio, which for small metallicity bins can be used as a proxy of relative age. This key relation is essential to constrain the formation mechanisms of the disc stellar populations as well as the cooling and settling processes. Methods: We used the recommended parameters and chemical abundances of 7800 FGK Milky Way field stars from the second internal data release of the Gaia-ESO spectroscopic Survey. These stars were observed with the GIRAFFE spectrograph (HR10 and HR21 setups), and cover a large spatial volume in the intervals 6 +0.20 dex for the radial component. First, the velocity dispersion increases with [Mg/Fe] at all [Fe/H] ratios for the thin-disc stars, and then it decreases for the thick-disc population at the highest [Mg/Fe] abundances. Similar trends are observed for several bins of [Mg/Fe] within the errors for the azimuthal velocity dispersion, while a continuous increase with [Mg/Fe] is observed for the vertical velocity dispersion. The velocity dispersion decrease agrees with previous measurements of the RAVE survey, although it is observed here for a greater metallicity interval and a larger spatial volume. Conclusions: Thanks to the Gaia-ESO Survey data, we confirm the existence of [Mg/Fe]-rich thick-disc stars with cool kinematics in the generally turbulent context of the primitive Galactic disc. This is discussed in the framework of the different disc formation and evolution scenarios. Based on observations collected with the FLAMES spectrograph at the VLT/UT2 telescope (Paranal Observatory, ESO, Chile), for the Gaia-ESO Large Public Survey, programme 188.B-3002

  9. Ghosts of Milky Way's past: the globular cluster ESO 37-1 (E 3)

    Science.gov (United States)

    de la Fuente Marcos, R.; de la Fuente Marcos, C.; Moni Bidin, C.; Ortolani, S.; Carraro, G.

    2015-09-01

    Context. In the Milky Way, most globular clusters are highly conspicuous objects that were found centuries ago. However, a few dozen of them are faint, sparsely populated systems that were identified largely during the second half of the past century. One of the faintest is ESO 37-1 (E 3) and as such it remains poorly studied, with no spectroscopic observations published so far although it was discovered in 1976. Aims: We investigate the globular cluster E 3 in an attempt to better constrain its fundamental parameters. Spectroscopy of stars in the field of E 3 is shown here for the first time. Methods: Deep, precise VI CCD photometry of E 3 down to V ~ 26 mag is presented and analysed. Low-resolution, medium signal-to-noise ratio spectra of nine candidate members are studied to derive radial velocity and metallicity. Proper motions from the UCAC4 catalogue are used to explore the kinematics of the bright members of E 3. Results: Isochrone fitting indicates that E 3 is probably very old, with an age of about 13 Gyr; its distance from the Sun is nearly 10 kpc. It is also somewhat metal rich with [Fe/H] = -0.7. Regarding its kinematics, our tentative estimate for the proper motions is (μα cosδ,μδ) = (-7.0 ± 0.8, 3.5 ± 0.3) mas yr-1 (or a tangential velocity of 382 ± 79 km s-1) and for the radial velocity 45 ± 5 km s-1 in the solar rest frame. Conclusions: E 3 is one of the most intriguing globular clusters in the Galaxy. Having an old age and being metal rich is clearly a peculiar combination, only seen in a handful of objects like the far more conspicuous NGC 104 (47 Tucanae). In addition, its low luminosity and sparse population make it a unique template for the study of the final evolutionary phases in the life of a star cluster. Unfortunately, E 3 is among the most elusive and challenging known globular clusters because field contamination severely hampers spectroscopic studies. This research note is based on observations made with the ESO VLT at the

  10. The Gaia-ESO Survey: Lithium enrichment histories of the Galactic thick and thin disc

    Science.gov (United States)

    Fu, X.; Romano, D.; Bragaglia, A.; Mucciarelli, A.; Lind, K.; Delgado Mena, E.; Sousa, S. G.; Randich, S.; Bressan, A.; Sbordone, L.; Martell, S.; Korn, A. J.; Abia, C.; Smiljanic, R.; Jofré, P.; Pancino, E.; Tautvaišienė, G.; Tang, B.; Magrini, L.; Lanzafame, A. C.; Carraro, G.; Bensby, T.; Damiani, F.; Alfaro, E. J.; Flaccomio, E.; Morbidelli, L.; Zaggia, S.; Lardo, C.; Monaco, L.; Frasca, A.; Donati, P.; Drazdauskas, A.; Chorniy, Y.; Bayo, A.; Kordopatis, G.

    2018-02-01

    Lithium abundance in most of the warm metal-poor main sequence stars shows a constarnt plateau (A(Li) 2.2 dex) and then the upper envelope of the lithium vs. metallicity distribution increases as we approach solar metallicity. Meteorites, which carry information about the chemical composition of the interstellar medium (ISM) at the solar system formation time, show a lithium abundance A(Li) 3.26 dex. This pattern reflects the Li enrichment history of the ISM during the Galaxy lifetime. After the initial Li production in big bang nucleosynthesis, the sources of the enrichment include asymptotic giant branch (AGB) stars, low-mass red giants, novae, type II supernovae, and Galactic cosmic rays. The total amount of enriched Li is sensitive to the relative contribution of these sources. Thus different Li enrichment histories are expected in the Galactic thick and thin disc. We investigate the main sequence stars observed with UVES in Gaia-ESO Survey iDR4 catalogue and find a Li-anticorrelation independent of [Fe/H], Teff, and log(g). Since in stellar evolution different α enhancements at the same metallicity do not lead to a measurable Li abundance change, the anticorrelation indicates that more Li is produced during the Galactic thin disc phase than during the Galactic thick disc phase. We also find a correlation between the abundance of Li and s-process elements Ba and Y, and they both decrease above the solar metallicity, which can be explained in the framework of the adopted Galactic chemical evolution models. The full Table 1 is only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/610/A38

  11. The Tumor Antigen NY-ESO-1 Mediates Direct Recognition of Melanoma Cells by CD4+ T Cells after Intercellular Antigen Transfer.

    Science.gov (United States)

    Fonteneau, Jean Francois; Brilot, Fabienne; Münz, Christian; Gannagé, Monique

    2016-01-01

    NY-ESO-1-specific CD4(+) T cells are of interest for immune therapy against tumors, because it has been shown that their transfer into a patient with melanoma resulted in tumor regression. Therefore, we investigated how NY-ESO-1 is processed onto MHC class II molecules for direct CD4(+) T cell recognition of melanoma cells. We could rule out proteasome and autophagy-dependent endogenous Ag processing for MHC class II presentation. In contrast, intercellular Ag transfer, followed by classical MHC class II Ag processing via endocytosis, sensitized neighboring melanoma cells for CD4(+) T cell recognition. However, macroautophagy targeting of NY-ESO-1 enhanced MHC class II presentation. Therefore, both elevated NY-ESO-1 release and macroautophagy targeting could improve melanoma cell recognition by CD4(+) T cells and should be explored during immunotherapy of melanoma. Copyright © 2015 by The American Association of Immunologists, Inc.

  12. Functional T cells targeting NY-ESO-1 or Melan-A are predictive for survival of patients with distant melanoma metastasis.

    Science.gov (United States)

    Weide, Benjamin; Zelba, Henning; Derhovanessian, Evelyna; Pflugfelder, Annette; Eigentler, Thomas K; Di Giacomo, Anna Maria; Maio, Michele; Aarntzen, Erik H J G; de Vries, I Jolanda M; Sucker, Antje; Schadendorf, Dirk; Büttner, Petra; Garbe, Claus; Pawelec, Graham

    2012-05-20

    To analyze the prognostic relevance of circulating T cells responding to NY-ESO-1, Melan-A, MAGE-3, and survivin in patients with melanoma with distant metastasis. We examined 84 patients with follow-up after analysis (cohort A), 18 long-term survivors with an extraordinarily favorable course of disease before analysis (> 24 months survival after first occurrence of distant metastases; cohort B), and 14 healthy controls. Circulating antigen-reactive T cells were characterized by intracellular cytokine staining after in vitro stimulation. In cohort A patients, the presence of T cells responding to peptides from NY-ESO-1, Melan-A, or MAGE-3 and the M category according to the American Joint Committee on Cancer classification were significantly associated with survival. T cells responding to NY-ESO-1 and Melan-A (hazard ratios, 0.29 and 0.18, respectively) remained independent prognostic factors in Cox regression analysis and were superior to the M category in predicting outcome. Median survival of patients possessing T cells responding to NY-ESO-1, Melan-A, or both was 21 months, compared with 6 months for all others. NY-ESO-1-responsive T cells were detected in 70% of cohort A patients surviving > 18 months and in 50% of cohort B patients. Melan-A responses were found in 42% and 47% of patients in cohorts A and B, respectively. In contrast, the proportion was only 22% for NY-ESO-1 and 23% for Melan-A in those who died within 6 months. The presence of circulating T cells responding to Melan-A or NY-ESO-1 had strong independent prognostic impact on survival in advanced melanoma. Our findings support the therapeutic relevance of Melan-A and NY-ESO-1 as targets for immunotherapy.

  13. Centrosomal localisation of the cancer/testis (CT) antigens NY-ESO-1 and MAGE-C1 is regulated by proteasome activity in tumour cells.

    Science.gov (United States)

    Pagotto, Anna; Caballero, Otavia L; Volkmar, Norbert; Devalle, Sylvie; Simpson, Andrew J G; Lu, Xin; Christianson, John C

    2013-01-01

    The Cancer/Testis (CT) antigen family of genes are transcriptionally repressed in most human tissues but are atypically re-expressed in many malignant tumour types. Their restricted expression profile makes CT antigens ideal targets for cancer immunotherapy. As little is known about whether CT antigens may be regulated by post-translational processing, we investigated the mechanisms governing degradation of NY-ESO-1 and MAGE-C1 in selected cancer cell lines. Inhibitors of proteasome-mediated degradation induced the partitioning of NY-ESO-1 and MAGE-C1 into a detergent insoluble fraction. Moreover, this treatment also resulted in increased localisation of NY-ESO-1 and MAGE-C1 at the centrosome. Despite their interaction, relocation of either NY-ESO-1 or MAGE-C1 to the centrosome could occur independently of each other. Using a series of truncated fragments, the regions corresponding to NY-ESO-1(91-150) and MAGE-C1(900-1116) were established as important for controlling both stability and localisation of these CT antigens. Our findings demonstrate that the steady state levels of NY-ESO-1 and MAGE-C1 are regulated by proteasomal degradation and that both behave as aggregation-prone proteins upon accumulation. With proteasome inhibitors being increasingly used as front-line treatment in cancer, these data raise issues about CT antigen processing for antigenic presentation and therefore immunogenicity in cancer patients.

  14. The potential of the CMB305 vaccine regimen to target NY-ESO-1 and improve outcomes for synovial sarcoma and myxoid/round cell liposarcoma patients.

    Science.gov (United States)

    Pollack, Seth M

    2018-02-01

    Synovial Sarcoma (SS) and Myxoid Round Cell Liposarcoma (MRCL) are devastating sarcoma subtypes with few treatment options and poor outcomes in the advanced setting. However, both these diseases may be ideal for novel immunotherapies targeting the cancer-testis antigen, NY-ESO-1. Areas covered: In this review, we discuss the novel NY-ESO-1 targeted vaccine regimen, CMB305. This regimen uses a unique integration-deficient, dendritic-cell targeting lentiviral vector from the ZVex® platform, LV305, in order to prime NY-ESO-1 specific T cells. LV305 has single agent activity, and, in one case, caused a durable partial response in a refractory SS patient. CMB305 also includes a boost from a NY-ESO-1 protein vaccine given along with a potent toll-like-4 receptor agonist, glycopyranosyl lipid A. CMB305 induces NY-ESO-1 specific T cell responses in both SS and MRC patients and these patients had excellent overall survival (OS) outcomes in the initial phase I study. Expert commentary: CMB305 is a therapeutic vaccine regimen targeting NY-ESO-1 based on the lentiviral vaccine vector, LV305. Phase I studies have proven this vaccine is active immunologically. Data suggesting this vaccine may improve OS for SS and MRCL patients is exciting but early, and on-going work is testing the impact of CMB305 on patient outcomes.

  15. El impacto del género en las elecciones académicas de los estudiantes asturianos que finalizan la ESO = Gender influence on academic choices of asturian students about to finalize ESO.

    OpenAIRE

    Rodríguez Menéndez, Mª del Carmen; Torío López, Susana; Fernández García, Carmen María

    2006-01-01

    En el presente artículo se estudia la persistencia de las diferencias de género que se producen en las elecciones académicas de los estudiantes asturianos que finalizan 4º de la ESO. Se examinan las causas que generan esa elección diferencial, haciendo especial hincapié en el constructo “autoeficacia ”como variable determinante en el proceso de elección vocacional. Se finaliza con algunas sugerencias que pueden facilitar la constitución de una orientación vocacional no sesgada por razón de gé...

  16. La competencia sobre planificación de investigaciones en 4º de ESO: un estudio de caso

    OpenAIRE

    Iñigo RODRÍGUEZ ARTECHE; Mª Mercedes MARTÍNEZ AZNAR; Mª Aránzazu GARITAGOITIA CID

    2016-01-01

    En los últimos años, las metodologías de tipo “Inquiry-Based Science Education” (IBSE) se vienen recomendando desde las instancias europeas para mejorar los niveles de competencia y las actitudes hacia la ciencia de los estudiantes. Así, el presente estudio pretende comprobar la eficacia de una metodología IBSE, la Metodología de Resolución de Problemas como Investigación (MRPI), para la enseñanza-aprendizaje de la competencia sobre planificación de investigaciones en 4º de ESO. Para ello, a ...

  17. La planificación de investigaciones y su evaluación en 4º de ESO

    OpenAIRE

    Martínez Aznar, Mª Mercedes; Rodríguez Arteche, Iñigo; Garitagoitia Cid, Mª Aránzazu

    2013-01-01

    En este trabajo se comprueba la eficacia de la resolución de situaciones problemáticas abiertas mediante la Metodología de Resolución de Problemas como Investigación (MRPI), de características Inquiry-Based Science Education, para el desarrollo de la competencia científica, en concreto de sus aspectos ligados a la planificación de investigaciones. A partir de las producciones de un grupo de escolares de 4º de ESO se analiza el progreso en los aprendizajes que permiten evaluar el grado de comp...

  18. The Gaia-ESO Survey. Mg-Al anti-correlation in iDR4 globular clusters

    Science.gov (United States)

    Pancino, E.; Romano, D.; Tang, B.; Tautvaišienė, G.; Casey, A. R.; Gruyters, P.; Geisler, D.; San Roman, I.; Randich, S.; Alfaro, E. J.; Bragaglia, A.; Flaccomio, E.; Korn, A. J.; Recio-Blanco, A.; Smiljanic, R.; Carraro, G.; Bayo, A.; Costado, M. T.; Damiani, F.; Jofré, P.; Lardo, C.; de Laverny, P.; Monaco, L.; Morbidelli, L.; Sbordone, L.; Sousa, S. G.; Villanova, S.

    2017-05-01

    We use Gaia-ESO (GES) Survey iDR4 data to explore the Mg-Al anti-correlation in globular clusters that were observed as calibrators, as a demonstration of the quality of Gaia-ESO Survey data and analysis. The results compare well with the available literature, within 0.1 dex or less, after a small (compared to the internal spreads) offset between the UVES and GIRAFFE data of 0.10-0.15 dex was taken into account. In particular, for the first time we present data for NGC 5927, which is one of the most metal-rich globular clusters studied in the literature so far with [ Fe / H ] = - 0.39 ± 0.04 dex; this cluster was included to connect with the open cluster regime in the Gaia-ESO Survey internal calibration. The extent and shape of the Mg-Al anti-correlation provide strong constraints on the multiple population phenomenon in globular clusters. In particular, we studied the dependency of the Mg-Al anti-correlation extension with metallicity, present-day mass,and age of the clusters, using GES data in combination with a large set of homogenized literature measurements.We find a dependency with both metallicity and mass, which is evident when fitting for the two parameters simultaneously, but we do not find significant dependency with age. We confirm that the Mg-Al anti-correlation is not seen in all clusters, but disappears for the less massive or most metal-rich clusters. We also use our data set to see whether a normal anti-correlation would explain the low [Mg/α] observed in some extragalactic globular clusters, but find that none of the clusters in our sample can reproduce it; a more extreme chemical composition, such as that of NGC 2419, would be required. We conclude that GES iDR4 data already meet the requirements set by the main survey goals and can be used to study globular clusters in detail, even if the analysis procedures were not specifically designed for them. Based on data products from observations made with ESO Telescopes at the La Silla Paranal

  19. Enseñanza de trigonometría en 4º de la ESO con GeoGebra

    OpenAIRE

    Díaz-Fernández, Maria Trinidad

    2014-01-01

    El presente trabajo tiene como objetivo principal presentar una propuesta didáctica basada en el uso de GeoGebra para favorecer la comprensión de la trigonometría en 4º de la ESO (opción B) y así mejorar el proceso de aprendizaje de esta rama que tantas dificultades presenta para los alumnos. Para realizar dicho trabajo se han desarrollado tres partes fundamentales: en primer lugar el marco teórico a través del cual se ha querido indagar sobre las dificultades psicológicas que conllevan los c...

  20. NY-ESO-1 autoantibody as a tumor-specific biomarker for esophageal cancer: screening in 1969 patients with various cancers.

    Science.gov (United States)

    Oshima, Yoko; Shimada, Hideaki; Yajima, Satoshi; Nanami, Tatsuki; Matsushita, Kazuyuki; Nomura, Fumio; Kainuma, Osamu; Takiguchi, Nobuhiro; Soda, Hiroaki; Ueda, Takeshi; Iizasa, Toshihiko; Yamamoto, Naoto; Yamamoto, Hiroshi; Nagata, Matsuo; Yokoi, Sana; Tagawa, Masatoshi; Ohtsuka, Seiko; Kuwajima, Akiko; Murakami, Akihiro; Kaneko, Hironori

    2016-01-01

    Although serum NY-ESO-1 antibodies (s-NY-ESO-1-Abs) have been reported in patients with esophageal carcinoma, this assay system has not been used to study a large series of patients with various other cancers. Serum samples of 1969 cancer patients [esophageal cancer (n = 172), lung cancer (n = 269), hepatocellular carcinoma (n = 91), prostate cancer (n = 358), gastric cancer (n = 313), colorectal cancer (n = 262), breast cancer (n = 365)] and 74 healthy individuals were analyzed using an originally developed enzyme-linked immunosorbent assay system for s-NY-ESO-1-Abs. The optical density cut-off value, determined as the mean plus three standard deviations for serum samples from the healthy controls, was fixed at 0.165. Conventional tumor markers were also evaluated in patients with esophageal carcinoma. The positive rate of s-NY-ESO-1-Abs in patients with esophageal cancer (31 %) was significantly higher than that in the other groups: patients with lung cancer (13 %), patients with hepatocellular carcinoma (11 %), patients with prostate cancer (10 %), patients with gastric cancer (10 %), patients with colorectal cancer (8 %), patients with breast cancer (7 %), and healthy controls (0 %). The positive rate of s-NY-ESO-1-Abs was comparable to that of serum p53 antibodies (33 %), squamous cell carcinoma antigen (36 %), carcinoembryonic antigen (26 %), and CYFRA 21-1 (18 %) and gradually increased with the tumor stage. The positive rate of s-NY-ESO-1-Abs was significantly higher in patients with esophageal cancer than in patients with the other types of cancers. On the basis of its high specificity and sensitivity, even in patients with stage I tumors, s-NY-ESO-1-Abs may be one of the first choices for esophageal cancer.

  1. Promoter hypomethylation of NY-ESO-1, association with clinicopathological features and PD-L1 expression in non-small cell lung cancer.

    Science.gov (United States)

    Chüeh, Anderly C; Liew, Mun-Sem; Russell, Prudence A; Walkiewicz, Marzena; Jayachandran, Aparna; Starmans, Maud H W; Boutros, Paul C; Wright, Gavin; Barnett, Stephen A; Mariadason, John M; John, Thomas

    2017-09-26

    Cancer-Testis antigens (CTA) are immunogenic molecules with normal tissue expression restricted to testes but with aberrant expression in up to 30% of non-small cell lung cancers (NSCLCs). Regulation of CTA expression is mediated in part through promoter DNA methylation. Recently, immunotherapy has altered treatment paradigms in NSCLC. Given its immunogenicity and ability to be re-expressed through demethylation, NY-ESO-1 promoter methylation, protein expression and its association with programmed death receptor ligand-1 (PD-L1) expression and clinicopathological features were investigated. Lung cancer cell line demethylation resulting from 5-Aza-2'-deoxycytidine treatment was associated with both NY-ESO-1 and PD-L1 re-expression in vitro but not increased chemosensitivity. NY-ESO-1 hypomethylation was observed in 15/94 (16%) of patient samples and associated with positive protein expression ( P ESO-1 and PD-L1 expression, despite resultant re-expression of both by 5-Aza-2'-deoxycytidine. Importantly, NY-ESO-1 hypomethylation was found to be an independent marker of poor prognosis in patients not treated with chemotherapy (HR 3.59, P = 0.003) in multivariate analysis. In patients treated with chemotherapy there were no differences in survival associated with NY-ESO-1 hypomethylation. Collectively, these results provided supporting evidence for the potential use of NY-ESO-1 hypomethylation as a prognostic biomarker in stage 3 NSCLCs. In addition, these data highlight the potential to incorporate demethylating agents to enhance immune activation, in tumours currently devoid of immune infiltrates and expression of immune checkpoint genes.

  2. A pilot trial using lymphocytes genetically engineered with an NY-ESO-1-reactive T-cell receptor: long-term follow-up and correlates with response.

    Science.gov (United States)

    Robbins, Paul F; Kassim, Sadik H; Tran, Thai L N; Crystal, Jessica S; Morgan, Richard A; Feldman, Steven A; Yang, James C; Dudley, Mark E; Wunderlich, John R; Sherry, Richard M; Kammula, Udai S; Hughes, Marybeth S; Restifo, Nicholas P; Raffeld, Mark; Lee, Chyi-Chia R; Li, Yong F; El-Gamil, Mona; Rosenberg, Steven A

    2015-03-01

    Although adoptive cell therapy can be highly effective for the treatment of patients with melanoma, the application of this approach to the treatment of other solid tumors has been limited. The observation that the cancer germline (CG) antigen NY-ESO-1 is expressed in 70% to 80% and in approximately 25% of patients with synovial cell sarcoma and melanoma, respectively, prompted us to perform this first-in-man clinical trial using the adoptive transfer of autologous peripheral blood mononuclear cells that were retrovirally transduced with an NY-ESO-1-reactive T-cell receptor (TCR) to heavily pretreated patients bearing these metastatic cancers. HLA-*0201 patients with metastatic synovial cell sarcoma or melanoma refractory to standard treatments and whose cancers expressed NY-ESO-1 received autologous TCR-transduced T cells following a lymphodepleting preparative chemotherapy. Response rates using Response Evaluation Criteria in Solid Tumors (RECIST), as well as immunologic correlates of response, are presented in this report. Eleven of 18 patients with NY-ESO-1(+) synovial cell sarcomas (61%) and 11 of 20 patients with NY-ESO-1(+) melanomas (55%) who received autologous T cells transduced with an NY-ESO-1-reactive TCR demonstrated objective clinical responses. The estimated overall 3- and 5-year survival rates for patients with synovial cell sarcoma were 38% and 14%, respectively, whereas the corresponding estimated survival rates for patients with melanoma were both 33%. The adoptive transfer of autologous T cells transduced with a retrovirus encoding a TCR against an HLA-A*0201 restricted NY-ESO-1 epitope can be an effective therapy for some patients bearing synovial cell sarcomas and melanomas that are refractory to other treatments. ©2014 American Association for Cancer Research.

  3. Efficacy of vaccination with recombinant vaccinia and fowlpox vectors expressing NY-ESO-1 antigen in ovarian cancer and melanoma patients.

    Science.gov (United States)

    Odunsi, Kunle; Matsuzaki, Junko; Karbach, Julia; Neumann, Antje; Mhawech-Fauceglia, Paulette; Miller, Austin; Beck, Amy; Morrison, Carl D; Ritter, Gerd; Godoy, Heidi; Lele, Shashikant; duPont, Nefertiti; Edwards, Robert; Shrikant, Protul; Old, Lloyd J; Gnjatic, Sacha; Jäger, Elke

    2012-04-10

    Recombinant poxviruses (vaccinia and fowlpox) expressing tumor-associated antigens are currently being evaluated in clinical trials as cancer vaccines to induce tumor-specific immune responses that will improve clinical outcome. To test whether a diversified prime and boost regimen targeting NY-ESO-1 will result in clinical benefit, we conducted two parallel phase II clinical trials of recombinant vaccinia-NY-ESO-1 (rV-NY-ESO-1), followed by booster vaccinations with recombinant fowlpox-NY-ESO-1 (rF-NY-ESO-1) in 25 melanoma and 22 epithelial ovarian cancer (EOC) patients with advanced disease who were at high risk for recurrence/progression. Integrated NY-ESO-1-specific antibody and CD4(+) and CD8(+) T cells were induced in a high proportion of melanoma and EOC patients. In melanoma patients, objective response rate [complete and partial response (CR+PR)] was 14%, mixed response was 5%, and disease stabilization was 52%, amounting to a clinical benefit rate (CBR) of 72% in melanoma patients. The median PFS in the melanoma patients was 9 mo (range, 0-84 mo) and the median OS was 48 mo (range, 3-106 mo). In EOC patients, the median PFS was 21 mo (95% CI, 16-29 mo), and median OS was 48 mo (CI, not estimable). CD8(+) T cells derived from vaccinated patients were shown to lyse NY-ESO-1-expressing tumor targets. These data provide preliminary evidence of clinically meaningful benefit for diversified prime and boost recombinant pox-viral-based vaccines in melanoma and ovarian cancer and support further evaluation of this approach in these patient populations.

  4. MAGEA4 expression in bone and soft tissue tumors: its utility as a target for immunotherapy and diagnostic marker combined with NY-ESO-1.

    Science.gov (United States)

    Iura, Kunio; Kohashi, Kenichi; Ishii, Takeaki; Maekawa, Akira; Bekki, Hirofumi; Otsuka, Hiroshi; Yamada, Yuichi; Yamamoto, Hidetaka; Matsumoto, Yoshihiro; Iwamoto, Yukihide; Oda, Yoshinao

    2017-07-26

    Cancer-testis (CT) antigens have promise as targets for immunotherapy, because of their restricted expression in tumor or testis tissue. MAGEA4 is both a MAGE family member and a CT antigen, and has attracted attention as a potential immunotherapeutic target. We investigated MAGEA4 expression by immunohistochemistry in bone and soft tissue tumor specimens that consisted of 35 malignant or intermediate and 24 benign histological subtypes, in order to evaluate its possible utility as an immunotherapy target and its potential use as a diagnostic marker when combined with another CT antigen, NY-ESO-1. Among these tumors, MAGEA4 was detected in 82.2% of synovial sarcomas, 67.7% of myxoid liposarcomas, 43.8% of osteosarcomas, 41.4% of angiosarcomas, 24.6% of malignant peripheral nerve sheath tumors (MPNSTs), and 21.4% of chondrosarcomas. NY-ESO-1 expression was found in 88.2% of myxoid liposarcomas, 61.1% of synovial sarcomas, 31.3% of osteosarcomas, 21.4% of pleomorphic liposarcomas, 16.7% of desmoplastic small round cell tumors, and 14.3% of chondrosarcomas. Benign tumors and non-tumorous tissue, except for testis tissue, did not express MAGEA4 or NY-ESO-1. Combined use of MAGEA4 and NY-ESO-1 increased the sensitivity, specificity, positive predictive values, and negative predictive values for distinguishing synovial sarcoma from spindle cell tumors and other mimicking tumors, compared to individual use of MAGEA4 or NY-ESO-1. Our results support the immunotherapy targeting MAGEA4 or NY-ESO-1 can be an ancillary therapy in the above-mentioned tumors, and the potential utility of MAGEA4 as an ancillary diagnostic marker for synovial sarcoma combined with NY-ESO-1.

  5. Prevalence of plasma autoantibody against cancer testis antigen NY-ESO-1 in HTLV-1 infected individuals with different clinical status.

    Science.gov (United States)

    Shiohama, Yasuo; Naito, Tadasuke; Matsuzaki, Toshio; Tanaka, Reiko; Tomoyose, Takeaki; Takashima, Hiroshi; Fukushima, Takuya; Tanaka, Yuetsu; Saito, Mineki

    2017-07-17

    Detection of specific immune responses against cancer/testis antigen NY-ESO-1 was recently reported in patients with adult T-cell leukemia/lymphoma (ATL) and human T-cell leukemia virus type 1 (HTLV-1)-infected asymptomatic carriers (ACs). However, the relationship of the responses with the HTLV-1 proviral load (PVL) and the levels of viral gene expression remain unclear. We measured plasma levels of autoantibodies to NY-ESO-1 immunogenic tumor antigen in HTLV-1-infected individuals with different clinical status, and in healthy controls. Data were compared to tax and HBZ mRNA levels, and PVL. Plasma anti-NY-ESO-1 antibody was detectable in 13.7% (7/51) of ACs, 29.2% (38/130) of patients with HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP), and 18.9% (10/53) of patients with ATL. Anti-NY-ESO-1 plasma levels were significantly higher in patients with HAM/TSP than in patients with ATL or ACs. Anti-NY-ESO-1 levels were not associated with PVL or the expression levels of tax and HBZ mRNA among HTLV-1-infected individuals, regardless of clinical status. The present results indicate the strong humoral immune response against NY-ESO-1 in natural HTLV-1 infection, irrespective of the clinical status. The higher immunoreactivity against NY-ESO-1 is not simply associated with the levels of both HTLV-1 gene expression and the number of infected cells in vivo. Rather, it might reflect chronic and generalized immune activation in infected individuals.

  6. Assessment of vaccine-induced CD4 T cell responses to the 119-143 immunodominant region of the tumor-specific antigen NY-ESO-1 using DRB1*0101 tetramers.

    Science.gov (United States)

    Ayyoub, Maha; Pignon, Pascale; Dojcinovic, Danijel; Raimbaud, Isabelle; Old, Lloyd J; Luescher, Immanuel; Valmori, Danila

    2010-09-15

    NY-ESO-1 (ESO), a tumor-specific antigen of the cancer/testis group, is presently viewed as an important model antigen for the development of generic anticancer vaccines. The ESO(119-143) region is immunodominant following immunization with a recombinant ESO vaccine. In this study, we generated DRB1*0101/ESO(119-143) tetramers and used them to assess CD4 T-cell responses in vaccinated patients expressing DRB1*0101 (DR1). We generated tetramers of DRB1*0101 incorporating peptide ESO(119-143) using a previously described strategy. We assessed ESO(119-143)-specific CD4 T cells in peptide-stimulated postvaccine cultures using the tetramers. We isolated DR1/ESO(119-143) tetramer(+) cells by cell sorting and characterized them functionally. We assessed vaccine-induced CD4(+) DR1/ESO(119-143) tetramer(+) T cells ex vivo and characterized them phenotypically. Staining of cultures from vaccinated patients with DR1/ESO(119-143) tetramers identified vaccine-induced CD4 T cells. Tetramer(+) cells isolated by cell sorting were of T(H)1 type and efficiently recognized full-length ESO. We identified ESO(123-137) as the minimal optimal epitope recognized by DR1-restricted ESO-specific CD4 T cells. By assessing DR1/ESO(119-143) tetramer(+) cells using T cell receptor (TCR) β chain variable region (Vβ)-specific antibodies, we identified several frequently used Vβ. Finally, direct ex vivo staining of patients' CD4 T cells with tetramers allowed the direct quantification and phenotyping of vaccine-induced ESO-specific CD4 T cells. The development of DR1/ESO(119-143) tetramers, allowing the direct visualization, isolation, and characterization of ESO-specific CD4 T cells, will be instrumental for the evaluation of spontaneous and vaccine-induced immune responses to this important tumor antigen in DR1-expressing patients. ©2010 AACR.

  7. ESPRESSO instrument control electronics: a PLC based distributed layout for a second generation instrument at ESO VLT

    Science.gov (United States)

    Baldini, V.; Cirami, R.; Coretti, I.; Cristiani, S.; Di Marcantonio, P.; Mannetta, M.; Santin, P.; Mégevand, D.; Zerbi, F.

    2014-07-01

    ESPRESSO is an ultra-stable fiber-fed spectrograph designed to combine incoherently the light coming from up to 4 Unit Telescopes of the ESO VLT. From the Nasmyth focus of each telescope the light, through an optical path, is fed by the Coudé Train subsystems to the Front End Unit placed in the Combined Coudé Laboratory. The Front End is composed by one arm for each telescope and its task is to convey the incoming light, after a calibration process, into the spectrograph fibers. To perform these operations a large number of functions are foreseen, like motorized stages, lamps, digital and analog sensors that, coupled with dedicated Technical CCDs (two per arms), allow to stabilize the incoming beam up to the level needed to exploit the ESPRESSO scientific requirements. The Instrument Control Electronics goal is to properly control all the functions in the Combined Coudé Laboratory and the spectrograph itself. It is fully based on a distributed PLC architecture, abandoning in this way the VME-based technology previously adopted for the ESO VLT instruments. In this paper we will describe the ESPRESSO Instrument Control Electronics architecture, focusing on the distributed layout and its interfaces with the other ESPRESSO subsystems.

  8. ESO Public Surveys at VISTA: Lessons learned from Cycle 1 Surveys and the start of Cycle 2

    Science.gov (United States)

    Arnaboldi, M.; Delmotte, N.; Gadotti, D.; Hilker, M.; Hussain, G.; Mascetti, L.; Micol, A.; Petr-Gotzens, M.; Rejkuba, M.; Retzlaff, J.; Ivison, R.; Leibundgut, B.; Romaniello, M.

    2017-06-01

    The ESO Public Surveys on VISTA serve the science goals of the survey teams while increasing the legacy value of ESO programmes, thanks to their homogeneity and the breadth of their sky coverage in multiple bands. These projects address a variety of research areas: from the detection of planets via microlensing, to stars, the Milky Way and Local Group galaxies, to extragalactic astronomy, galaxy evolution, the high-redshift Universe and cosmology. In 2015, as the first generation of imaging surveys was nearing completion, a second call for Public Surveys was opened to define a coherent scientific programme for VISTA until the commissioning of the wide-field multi-fibre spectrograph, 4MOST, in 2020. This article presents the status of the Cycle 1 surveys as well as an overview of the seven new programmes in Cycle 2, including their science goals, coverage on the sky and observing strategies. We conclude with a forward look at the Cycle 2 data releases and the timelines for their release.

  9. Bases para la elaboracion de unidades didacticas de calidad en el area de ciencias (Fisica y Quimica 3 deg ESO)

    Science.gov (United States)

    Maccioni, Elena Lucia

    Este Trabajo Fin de Master tiene como objeto, el estudio previo de la educacion de la ciencia en la actualidad y mas destacable, del diseno de las unidades didacticas segun las metodologias mas frecuentes aplicadas por los diferentes sistemas educativos en el area de las Ciencias teniendo en cuenta la importancia y el efecto de la psicologia del alumnado y todo ello como no puede ser de otro modo bajo el corse de la Legislacion aplicable, Estatal, Autonomica y europea. Con estos antecedentes, se extrae cuales son las preguntas que deben contestarse en la elaboracion de una unidad didactica de calidad en el contexto, generacional (edad y sexo), del Proyecto Educativo de Centro, y de la Programacion del Departamento, discutiendo y justificando cada uno de los apartados en que estructuradamente dividiremos dicha Unidad, con un formato manejable, util, y dinamico en el tiempo que sea un verdadero instrumento educativo de aula. Teniendo en cuenta estas premisas, se procede a hacer una recopilacion de una misma unidad didactica publicada por diferentes editoriales, elegidas no al azar, sino en funcion de su penetracion editorial en nuestros centros de ESO, siendo las elegidas (SM y Oxford). Las diferentes unidades seleccionadas son analizadas de forma critica, atendiendo a los criterios generales de calidad bajo parametros cientificos y normativos, concluyendo con la aportacion final que es la redaccion de unas pautas cientifico-pedagogicas, para redactar unidades didacticas de calidad en el area de la Ciencias, en concreto en la Asignatura de Fisica y Quimica de 3º de ESO.

  10. Expression and clinical significance of MAGE and NY-ESO-1 cancer-testis antigens in adenoid cystic carcinoma of the head and neck.

    Science.gov (United States)

    Veit, Johannes A; Heine, Daniela; Thierauf, Julia; Lennerz, Jochen; Shetty, Subasch; Schuler, Patrick J; Whiteside, Theresa; Beutner, Dirk; Meyer, Moritz; Grünewald, Inga; Ritter, Gerd; Gnjatic, Sacha; Sikora, Andrew G; Hoffmann, Thomas K; Laban, Simon

    2016-07-01

    Adenoid cystic carcinoma (ACC) of the head and neck is a rare but highly malignant tumor. Cancer-testis antigens (CTAs) represent an immunogenic family of cancer-specific proteins and thus represent an attractive target for immunotherapy. Eighty-four cases of ACC were identified, the CTAs pan-Melanoma antigen (pan-MAGE; M3H67) and New York esophageal squamous cell carcinoma (NY-ESO-1; E978) were detected immunohistochemically (IHC) and correlated with clinical data. Expression of NY-ESO-1 was found in 48 of 84 patients (57.1%) and of pan-MAGE in 28 of 84 patients (31.2%). Median overall survival (OS) in NY-ESO-1 positive versus negative patients was 130.8 and 282.0 months (p = .223), respectively. OS in pan-MAGE positive versus negative patients was 105.3 and 190.5 months, respectively (p = .096). Patients expressing both NY-ESO-1 and pan-MAGE simultaneously had significantly reduced OS with a median of 90.5 months compared with 282.0 months in negative patients (p = .047). A significant fraction of patients with ACC show expression of the CTAs NY-ESO-1 and/or pan-MAGE with promising immunotherapeutic implications. © 2016 Wiley Periodicals, Inc. Head Neck 38: 1008-1016, 2016. © 2016 Wiley Periodicals, Inc.

  11. Lack of XAGE-1b and NY-ESO-1 in metastatic lymph nodes may predict the potential survival of stage III melanoma patients.

    Science.gov (United States)

    Mori, Mariko; Funakoshi, Takeru; Kameyama, Kaori; Kawakami, Yutaka; Sato, Eiichi; Nakayama, Eiichi; Amagai, Masayuki; Tanese, Keiji

    2017-06-01

    The cancer-testis antigens (CTA) are a large family of tumor-associated antigens expressed by a variety of cancer cells and primitive germ cells of the adult testis and placenta. These tumor-restricted expressing patterns suggest that CTA would be ideal targets for tumor-specific immunotherapy. XAGE-1 is a CTA that was originally identified by computer-based screening, and four transcription variants, XAGE-1a, -1b, -1c and -1d, have been characterized to date. Although the presence of XAGE-1 transcripts has been reported in various cancers, the expression of XAGE-1b in melanoma has not been fully characterized. In this study, we performed immunohistochemical staining of XAGE-1b together with NY-ESO-1, a well-known CTA, in 113 melanoma samples obtained from 84 patients and evaluated their expression in tumor cells. The effects of expression on tumor progression and patient prognosis were analyzed. Both XAGE-1b and NY-ESO-1 were expressed at high levels in lymph node metastasis and skin metastasis samples compared with the primary site (P ESO-1). In a subgroup analysis of 22 patients with stage III lymph node metastasis, overall survival was significantly higher in the XAGE-1b and NY-ESO-1 double-negative group than in the other groups (P ESO-1 expression could have a positive influence on clinical outcome in patients with melanoma. © 2017 Japanese Dermatological Association.

  12. The Gaia-ESO Survey: The analysis of high-resolution UVES spectra of FGK-type stars

    Science.gov (United States)

    Smiljanic, R.; Korn, A. J.; Bergemann, M.; Frasca, A.; Magrini, L.; Masseron, T.; Pancino, E.; Ruchti, G.; San Roman, I.; Sbordone, L.; Sousa, S. G.; Tabernero, H.; Tautvaišienė, G.; Valentini, M.; Weber, M.; Worley, C. C.; Adibekyan, V. Zh.; Allende Prieto, C.; Barisevičius, G.; Biazzo, K.; Blanco-Cuaresma, S.; Bonifacio, P.; Bragaglia, A.; Caffau, E.; Cantat-Gaudin, T.; Chorniy, Y.; de Laverny, P.; Delgado-Mena, E.; Donati, P.; Duffau, S.; Franciosini, E.; Friel, E.; Geisler, D.; González Hernández, J. I.; Gruyters, P.; Guiglion, G.; Hansen, C. J.; Heiter, U.; Hill, V.; Jacobson, H. R.; Jofre, P.; Jönsson, H.; Lanzafame, A. C.; Lardo, C.; Ludwig, H.-G.; Maiorca, E.; Mikolaitis, Š.; Montes, D.; Morel, T.; Mucciarelli, A.; Muñoz, C.; Nordlander, T.; Pasquini, L.; Puzeras, E.; Recio-Blanco, A.; Ryde, N.; Sacco, G.; Santos, N. C.; Serenelli, A. M.; Sordo, R.; Soubiran, C.; Spina, L.; Steffen, M.; Vallenari, A.; Van Eck, S.; Villanova, S.; Gilmore, G.; Randich, S.; Asplund, M.; Binney, J.; Drew, J.; Feltzing, S.; Ferguson, A.; Jeffries, R.; Micela, G.; Negueruela, I.; Prusti, T.; Rix, H.-W.; Alfaro, E.; Babusiaux, C.; Bensby, T.; Blomme, R.; Flaccomio, E.; François, P.; Irwin, M.; Koposov, S.; Walton, N.; Bayo, A.; Carraro, G.; Costado, M. T.; Damiani, F.; Edvardsson, B.; Hourihane, A.; Jackson, R.; Lewis, J.; Lind, K.; Marconi, G.; Martayan, C.; Monaco, L.; Morbidelli, L.; Prisinzano, L.; Zaggia, S.

    2014-10-01

    Context. The ongoing Gaia-ESO Public Spectroscopic Survey is using FLAMES at the VLT to obtain high-quality medium-resolution Giraffe spectra for about 105 stars and high-resolution UVES spectra for about 5000 stars. With UVES, the Survey has already observed 1447 FGK-type stars. Aims: These UVES spectra are analyzed in parallel by several state-of-the-art methodologies. Our aim is to present how these analyses were implemented, to discuss their results, and to describe how a final recommended parameter scale is defined. We also discuss the precision (method-to-method dispersion) and accuracy (biases with respect to the reference values) of the final parameters. These results are part of the Gaia-ESO second internal release and will be part of its first public release of advanced data products. Methods: The final parameter scale is tied to the scale defined by the Gaia benchmark stars, a set of stars with fundamental atmospheric parameters. In addition, a set of open and globular clusters is used to evaluate the physical soundness of the results. Each of the implemented methodologies is judged against the benchmark stars to define weights in three different regions of the parameter space. The final recommended results are the weighted medians of those from the individual methods. Results: The recommended results successfully reproduce the atmospheric parameters of the benchmark stars and the expected Teff-log g relation of the calibrating clusters. Atmospheric parameters and abundances have been determined for 1301 FGK-type stars observed with UVES. The median of the method-to-method dispersion of the atmospheric parameters is 55 K for Teff, 0.13 dex for log g and 0.07 dex for [Fe/H]. Systematic biases are estimated to be between 50-100 K for Teff, 0.10-0.25 dex for log g and 0.05-0.10 dex for [Fe/H]. Abundances for 24 elements were derived: C, N, O, Na, Mg, Al, Si, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Y, Zr, Mo, Ba, Nd, and Eu. The typical method

  13. NY-ESO-1-specific circulating CD4+ T cells in ovarian cancer patients are prevalently T(H)1 type cells undetectable in the CD25+ FOXP3+ Treg compartment.

    Science.gov (United States)

    Redjimi, Nassima; Duperrier-Amouriaux, Karine; Raimbaud, Isabelle; Luescher, Immanuel; Dojcinovic, Danijel; Classe, Jean-Marc; Berton-Rigaud, Dominique; Frenel, Jean-Sébastien; Bourbouloux, Emmanuelle; Valmori, Danila; Ayyoub, Maha

    2011-01-01

    Spontaneous CD4(+) T-cell responses to the tumor-specific antigen NY-ESO-1 (ESO) are frequently found in patients with epithelial ovarian cancer (EOC). If these responses are of effector or/and Treg type, however, has remained unclear. Here, we have used functional approaches together with recently developed MHC class II/ESO tetramers to assess the frequency, phenotype and function of ESO-specific cells in circulating lymphocytes from EOC patients. We found that circulating ESO-specific CD4(+) T cells in EOC patients with spontaneous immune responses to the antigen are prevalently T(H)1 type cells secreting IFN-γ but no IL-17 or IL-10 and are not suppressive. We detected tetramer(+) cells ex vivo, at an average frequency of 1:25,000 memory cells, that is, significantly lower than in patients immunized with an ESO vaccine. ESO tetramer(+) cells were mostly effector memory cells at advanced stages of differentiation and were not detected in circulating CD25(+)FOXP3(+)Treg. Thus, spontaneous CD4(+) T-cell responses to ESO in cancer patients are prevalently of T(H)1 type and not Treg. Their relatively low frequency and advanced differentiation stage, however, may limit their efficacy, that may be boosted by immunogenic ESO vaccines.

  14. NY-ESO-1-Specific Circulating CD4+ T Cells in Ovarian Cancer Patients Are Prevalently TH1 Type Cells Undetectable in the CD25+FOXP3+Treg Compartment

    Science.gov (United States)

    Redjimi, Nassima; Duperrier-Amouriaux, Karine; Raimbaud, Isabelle; Luescher, Immanuel; Dojcinovic, Danijel; Classe, Jean-Marc; Berton-Rigaud, Dominique; Frenel, Jean-Sébastien; Bourbouloux, Emmanuelle

    2011-01-01

    Spontaneous CD4+ T-cell responses to the tumor-specific antigen NY-ESO-1 (ESO) are frequently found in patients with epithelial ovarian cancer (EOC). If these responses are of effector or/and Treg type, however, has remained unclear. Here, we have used functional approaches together with recently developed MHC class II/ESO tetramers to assess the frequency, phenotype and function of ESO-specific cells in circulating lymphocytes from EOC patients. We found that circulating ESO-specific CD4+ T cells in EOC patients with spontaneous immune responses to the antigen are prevalently TH1 type cells secreting IFN-γ but no IL-17 or IL-10 and are not suppressive. We detected tetramer+ cells ex vivo, at an average frequency of 1∶25000 memory cells, that is, significantly lower than in patients immunized with an ESO vaccine. ESO tetramer+ cells were mostly effector memory cells at advanced stages of differentiation and were not detected in circulating CD25+FOXP3+Treg. Thus, spontaneous CD4+ T-cell responses to ESO in cancer patients are prevalently of TH1 type and not Treg. Their relatively low frequency and advanced differentiation stage, however, may limit their efficacy, that may be boosted by immunogenic ESO vaccines. PMID:21829534

  15. The Gaia-ESO Survey: dynamics of ionized and neutral gas in the Lagoon nebula (M 8)

    Science.gov (United States)

    Damiani, F.; Bonito, R.; Prisinzano, L.; Zwitter, T.; Bayo, A.; Kalari, V.; Jiménez-Esteban, F. M.; Costado, M. T.; Jofré, P.; Randich, S.; Flaccomio, E.; Lanzafame, A. C.; Lardo, C.; Morbidelli, L.; Zaggia, S.

    2017-08-01

    Aims: We present a spectroscopic study of the dynamics of the ionized and neutral gas throughout the Lagoon nebula (M 8), using VLT-FLAMES data from the Gaia-ESO Survey. The new data permit exploration of the physical connections between the nebular gas and the stellar population of the associated star cluster NGC 6530. Methods: We characterized through spectral fitting emission lines of Hα, [N II] and [S II] doublets, [O III], and absorption lines of sodium D doublet, using data from the FLAMES-Giraffe and UVES spectrographs, on more than 1000 sightlines toward the entire face of the Lagoon nebula. Gas temperatures are derived from line-width comparisons, densities from the [S II] doublet ratio, and ionization parameter from Hα/[N II] ratio. Although doubly-peaked emission profiles are rarely found, line asymmetries often imply multiple velocity components along the same line of sight. This is especially true for the sodium absorption, and for the [O III] lines. Results: Spatial maps for density and ionization are derived, and compared to other known properties of the nebula and of its massive stars 9 Sgr, Herschel 36 and HD 165052 which are confirmed to provide most of the ionizing flux. The detailed velocity fields across the nebula show several expanding shells, related to the cluster NGC 6530, the O stars 9 Sgr and Herschel 36, and the massive protostar M 8East-IR. The origins of kinematical expansion and ionization of the NGC 6530 shell appear to be different. We are able to put constrains on the line-of-sight (relative or absolute) distances between some of these objects and the molecular cloud. The data show that the large obscuring band running through the middle of the nebula is being compressed by both sides, which might explain its enhanced density. We also find an unexplained large-scale velocity gradient across the entire nebula. At larger distances, the transition from ionized to neutral gas is studied using the sodium lines. Based on observations

  16. Gaia-ESO Survey: Global properties of clusters Trumpler 14 and 16 in the Carina nebula ⋆⋆

    Science.gov (United States)

    Damiani, F.; Klutsch, A.; Jeffries, R. D.; Randich, S.; Prisinzano, L.; Maíz Apellániz, J.; Micela, G.; Kalari, V.; Frasca, A.; Zwitter, T.; Bonito, R.; Gilmore, G.; Flaccomio, E.; Francois, P.; Koposov, S.; Lanzafame, A. C.; Sacco, G. G.; Bayo, A.; Carraro, G.; Casey, A. R.; Alfaro, E. J.; Costado, M. T.; Donati, P.; Franciosini, E.; Hourihane, A.; Jofré, P.; Lardo, C.; Lewis, J.; Magrini, L.; Monaco, L.; Morbidelli, L.; Worley, C. C.; Vink, J. S.; Zaggia, S.

    2017-07-01

    Aims: We present the first extensive spectroscopic study of the global population in star clusters Trumpler 16, Trumpler 14, and Collinder 232 in the Carina nebula, using data from the Gaia-ESO Survey, down to solar-mass stars. Methods: In addition to the standard homogeneous survey data reduction, a special processing was applied here because of the bright nebulosity surrounding Carina stars. Results: We find about 400 good candidate members ranging from OB types down to slightly subsolar masses. About 100 heavily reddened early-type Carina members found here were previously unrecognized or poorly classified, including two candidate O stars and several candidate Herbig Ae/Be stars. Their large brightness makes them useful tracers of the obscured Carina population. The spectroscopically derived temperatures for nearly 300 low-mass members enables the inference of individual extinction values and the study of the relative placement of stars along the line of sight. Conclusions: We find a complex spatial structure with definite clustering of low-mass members around the most massive stars and spatially variable extinction. By combining the new data with existing X-ray data, we obtain a more complete picture of the three-dimensional spatial structure of the Carina clusters and of their connection to bright and dark nebulosity and UV sources. The identification of tens of background giants also enables us to determine the total optical depth of the Carina nebula along many sightlines. We are also able to put constraints on the star formation history of the region with Trumpler 14 stars found to be systematically younger than stars in other subclusters. We find a large percentage of fast-rotating stars among Carina solar-mass members, which provide new constraints on the rotational evolution of pre-main-sequence stars in this mass range. Based on observations collected with the FLAMES spectrograph at VLT/UT2 telescope (Paranal Observatory, ESO, Chile), for the Gaia-ESO

  17. Bi-specific TCR-anti CD3 redirected T-cell targeting of NY-ESO-1- and LAGE-1-positive tumors

    OpenAIRE

    McCormack, Emmet; Adams, Katherine J.; Hassan, Namir J.; Kotian, Akhil; Lissin, Nikolai M.; Sami, Malkit; Muji?, Maja; Osdal, Tereza; Gjertsen, Bj?rn Tore; Baker, Deborah; Powlesland, Alex S.; Aleksic, Milos; Vuidepot, Annelise; Morteau, Olivier; Sutton, Deborah H.

    2012-01-01

    NY-ESO-1 and LAGE-1 are cancer testis antigens with an ideal profile for tumor immunotherapy, combining up-regulation in many cancer types with highly restricted expression in normal tissues and sharing a common HLA-A*0201 epitope, 157?165. Here, we present data to describe the specificity and anti-tumor activity of a bifunctional ImmTAC, comprising a soluble, high-affinity T-cell receptor (TCR) specific for NY-ESO-1157?165 fused to an anti-CD3 scFv. This reagent, ImmTAC-NYE, is shown to kill...

  18. El diodo como operador elemental en circuitos electrónicos. Una propuesta para su enseñanza enel 2º ciclo de ESO

    Directory of Open Access Journals (Sweden)

    Antonio García-Carmona

    2005-01-01

    Full Text Available En este trabajo se expone la necesidad de impulsar el estudio de la Electrónica en la ESO, con el fin de contribuir en la formación básica e integral de los alumnos de este nivel, que están inmersos en una sociedad tecnológicamente avanzada. Y, como consecuencia de esto, presentamos una propuesta didáctica orientada al estudio del diodo en el 2º ciclo de ESO, en el marco de los currículos de Ciencias (Física y Química y de Tecnología

  19. High Resolution Infrared Spectroscopy in Astronomy Proceedings of an ESO Workshop Held at Garching, Germany, 18-21 November 2003

    CERN Document Server

    Käufl, Hans Ulrich; Moorwood, Alan F. M

    2005-01-01

    Two specialized new instruments for ESO's VLT, VISIR and CRIRES, spawned the idea for this workshop. CRIRES is a dedicated very high resolution infrared spectrograph; VISIR features a high resolution spectroscopic mode. Together, the instruments combine the sensitivity of an 8m-telescope with the now well-established reliability of VLT-facility instruments. High resolution here means that lines in cool stellar atmospheres and HII-regions can be resolved. The astrophysical topics discussed in this rather specialized workshop range from the inner solar system to active galactic nuclei. There are many possibilities for new discoveries with these instruments, but the unique capability, which becomes available through high-resolution infrared spectroscopy, is the observation of molecular rotational-vibrational transitions in many astrophysical environments. Particularly interesting and surprising in this context, many papers on modeling and laboratory spectroscopy at the workshop appear to indicate that astronomic...

  20. ESO's early history, 1953 - 1975. V. Earliest developments in Chile; 24 March 1966: the road on La Silla dedicated.

    Science.gov (United States)

    Blaauw, A.

    1989-12-01

    While - as described in the previous article - in Europe Directorate and Council established ESO's administrative basis, and the first telescopes were built or acquired under the guidance of the Instrumentation Committee, work in Chile proceeded equally energetically. Under the leadership of Andre Muller, since January 1, 1964 Superintendent for Chile, a great variety of tasks had to be taken up: building up staff for administration and construction, organizing office facilities, setting up temporary camps as basis for the activities on and around La Silla, learning to know the Chilean world of government and Drovincial authorities-and of contractors, etc. A challenging but demanding assignment! For it is one thing to build up an organization in one's own country with its well-known legal structure and social traditions - but another one to do so in a foreign country with unfamiliar language, different customs and different rules.

  1. ESTUDIO SOBRE PRÁCTICAS DOCENTES EN EVALUACIÓN DE LA LENGUA INGLESA EN LA ESO

    Directory of Open Access Journals (Sweden)

    Fernando D. Rubio

    2012-01-01

    Full Text Available Se critica la enseñanza del inglés en España y los resultados de aprendizaje. En este artículo se advierte que en parte se debe a la ausencia de evaluación oral en la ESO, y para ello se han analizado las prácticas docentes de evaluación. Se recogieron instrumentos de evaluación utilizados, en nuestro caso exámenes, y se analizaron sus contenidos. Los resultados obtenidos indican que las destrezas orales apenas se evalúan, que los elementos comunicativos son casi inexistentes y que no se siguen algunos criterios de evaluación marcados por la ley.

  2. (Liquid + liquid) equilibrium for ternary mixtures of {heptane + aromatic compounds + [EMpy][ESO4]} at T = 298.15 K

    International Nuclear Information System (INIS)

    Mirkhani, S.A.; Vossoughi, M.; Pazuki, G.R.; Safekordi, A.A.; Heydari, A.; Akbari, J.; Yavari, M.

    2011-01-01

    Highlights: → This paper reports the LLE data of ternary systems {heptane (1) + aromatic compounds (2) + [EMpy][ESO 4 ] (3)}. → The distribution coefficient and the selectivity were obtained from the experimental data. → The consistency of LLE data was successfully correlated with Othmer-Tobias and Hand equation. - Abstract: (Liquid + liquid) equilibrium (LLE) data for the ternary systems (heptane + toluene + 1-ethyl-3-methylpyridinium ethylsulfate) and (heptane + benzene + 1-ethyl-3-methylpyridinium ethylsulfate) were measured at T = 298.15 K and atmospheric pressure. The selectivity and aromatic distribution coefficients, calculated from the equilibrium data, were used to determine if this ionic liquid can be used as a potential extracting solvent for the separation of aromatic compounds from heptane. The consistency of tie-line data was ascertained by applying the Othmer-Tobias and Hand equations.

  3. Utilización del modelo Flipped Classroom en trigonometría de 4º de la ESO

    OpenAIRE

    Grané-de Dalmau, Marc

    2016-01-01

    El trabajo que se presenta a continuación tiene como objetivo principal elaborar una propuesta didáctica para la asignatura de matemáticas, opción de enseñanzas académicas, en 4º de la ESO. La propuesta se plantea para trabajar el tema de trigonometría y se basa en la utilización del modelo Flipped Classroom. A partir de la determinación de un problema de investigación se elabora un marco teórico que sirve de fundamento para la realización de la propuesta didáctica. Éste permite conocer con p...

  4. A 12 μm ISOCAM survey of the ESO-Sculptor field. Data reduction and analysis

    Science.gov (United States)

    Seymour, N.; Rocca-Volmerange, B.; de Lapparent, V.

    2007-12-01

    We present a detailed reduction of a mid-infrared 12 μm (LW10 filter) ISOCAM open time observation performed on the ESO-Sculptor Survey field (Arnouts et al. 1997, A&AS, 124, 163). A complete catalogue of 142 sources (120 galaxies and 22 stars), detected with high significance (equivalent to 5σ), is presented above an integrated flux density of 0.24 {mJy}. Star/galaxy separation is performed by a detailed study of colour-colour diagrams. The catalogue is complete to 1 {mJy} and, below this flux density, the incompleteness is corrected using two independent methods. The first method uses stars and the second uses optical counterparts of the ISOCAM galaxies; these methods yield consistent results. We also apply an empirical flux density calibration using stars in the field. For each star, the 12 μm flux density is derived by fitting optical colours from a multi-band χ2 to stellar templates (BaSel-2.0) and using empirical optical-IR colour-colour relations. This article is a companion analysis to our 2007 paper (Rocca-Volmerange et al. 2007, A&A, 475, 801) where the 12 μ m faint galaxy counts are presented and analysed per galaxy type with the evolutionary code PÉGASE.3. Based on observations collected at the European Southern Observatory (ESO), La Silla, Chile, and on observations with ISO, an ESA project with instruments funded by ESA Member States (especially the PI countries: France, Germany, the Netherlands, and the United Kingdom) and with the participation of ISAS and NASA. Full Table [see full textsee full textsee full textsee full textsee full textsee full text] is only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/cgi-bin/qcat?J/A+A/475/791

  5. LV305, a dendritic cell-targeting integration-deficient ZVex(TM)-based lentiviral vector encoding NY-ESO-1, induces potent anti-tumor immune response.

    Science.gov (United States)

    Albershardt, Tina Chang; Campbell, David James; Parsons, Andrea Jean; Slough, Megan Merrill; Ter Meulen, Jan; Berglund, Peter

    2016-01-01

    We have engineered an integration-deficient lentiviral vector, LV305, to deliver the tumor antigen NY-ESO-1 to human dendritic cells in vivo through pseudotyping with a modified Sindbis virus envelop protein. Mice immunized once with LV305 developed strong, dose-dependent, multifunctional, and cytotoxic NY-ESO-1-specific cluster of differentiation 8 (CD8) T cells within 14 days post-immunization and could be boosted with LV305 at least twice to recall peak-level CD8 T-cell responses. Immunization with LV305 protected mice against tumor growth in an NY-ESO-1-expressing CT26 lung metastasis model, with the protective effect abrogated upon depletion of CD8 T cells. Adoptive transfer of CD8 T cells, alone or together with CD4 T cells or natural killer cells, from LV305-immunized donor mice to tumor-bearing recipient mice conferred significant protection against metastatic tumor growth. Biodistribution of injected LV305 in mice was limited to the site of injection and the draining lymph node, and injected LV305 exhibited minimal excretion. Mice injected with LV305 developed little to no adverse effects, as evaluated by toxicology studies adherent to good laboratory practices. Taken together, these data support the development of LV305 as a clinical candidate for treatment against tumors expressing NY-ESO-1.

  6. Analysis of GAGE, NY-ESO-1 and SP17 cancer/testis antigen expression in early stage non-small cell lung carcinoma

    DEFF Research Database (Denmark)

    Gjerstorff, Morten F; Pøhl, Mette; Olsen, Karen E

    2013-01-01

    NSCLC has shown promising results. However, little is known about the expression of other cancer/testis antigens in NSCLC. In the present study the expression of cancer/testis antigens GAGE, NY-ESO-1 and SP17 was investigated in patients with completely resected, early stage, primary NSCLC....

  7. LV305, a dendritic cell-targeting integration-deficient ZVexTM-based lentiviral vector encoding NY-ESO-1, induces potent anti-tumor immune response

    Science.gov (United States)

    Albershardt, Tina Chang; Campbell, David James; Parsons, Andrea Jean; Slough, Megan Merrill; ter Meulen, Jan; Berglund, Peter

    2016-01-01

    We have engineered an integration-deficient lentiviral vector, LV305, to deliver the tumor antigen NY-ESO-1 to human dendritic cells in vivo through pseudotyping with a modified Sindbis virus envelop protein. Mice immunized once with LV305 developed strong, dose-dependent, multifunctional, and cytotoxic NY-ESO-1-specific cluster of differentiation 8 (CD8) T cells within 14 days post-immunization and could be boosted with LV305 at least twice to recall peak-level CD8 T-cell responses. Immunization with LV305 protected mice against tumor growth in an NY-ESO-1-expressing CT26 lung metastasis model, with the protective effect abrogated upon depletion of CD8 T cells. Adoptive transfer of CD8 T cells, alone or together with CD4 T cells or natural killer cells, from LV305-immunized donor mice to tumor-bearing recipient mice conferred significant protection against metastatic tumor growth. Biodistribution of injected LV305 in mice was limited to the site of injection and the draining lymph node, and injected LV305 exhibited minimal excretion. Mice injected with LV305 developed little to no adverse effects, as evaluated by toxicology studies adherent to good laboratory practices. Taken together, these data support the development of LV305 as a clinical candidate for treatment against tumors expressing NY-ESO-1. PMID:27626061

  8. Analysis of GAGE, NY-ESO-1 and SP17 cancer/testis antigen expression in early stage non-small cell lung carcinoma.

    Science.gov (United States)

    Gjerstorff, Morten F; Pøhl, Mette; Olsen, Karen E; Ditzel, Henrik J

    2013-10-08

    The unique expression pattern and immunogenic properties of cancer/testis antigens make them ideal targets for immunotherapy of cancer. The MAGE-A3 cancer/testis antigen is frequently expressed in non-small cell lung cancer (NSCLC) and vaccination with MAGE-A3 in patients with MAGE-A3-positive NSCLC has shown promising results. However, little is known about the expression of other cancer/testis antigens in NSCLC. In the present study the expression of cancer/testis antigens GAGE, NY-ESO-1 and SP17 was investigated in patients with completely resected, early stage, primary NSCLC. Tumor biopsies from normal lung tissue and from a large cohort (n = 169) of NSCLC patients were examined for GAGE, NY-ESO-1 and SP17 protein expression by immunohistochemical analysis. The expression of these antigens was further matched to clinical and pathological features using univariate cox regression analysis. GAGE and NY-ESO-1 cancer/testis antigens were not expressed in normal lung tissue, while SP17 was expressed in ciliated lung epithelia. The frequency of GAGE, NY-ESO-1 and SP17 expression in NSCLC tumors were 26.0% (44/169), 11.8% (20/169) and 4.7% (8/169), respectively, and 33.1% (56/169) of the tumors expressed at least one of these antigens. In general, the expression of GAGE, NY-ESO-1 and SP17 was not significantly associated with a specific histotype (adenocarcinoma vs. squamous cell carcinoma), but high-level GAGE expression (>50%) was more frequent in squamous cell carcinoma (p = 0.02). Furthermore, the frequency of GAGE expression was demonstrated to be significantly higher in stage II-IIIa than stage I NSCLC (17.0% vs. 35.8%; p = 0.02). Analysis of the relation between tumor expression of GAGE and NY-ESO-1 and survival endpoints revealed no significant associations. Our study demonstrates that GAGE, NY-ESO-1 and SP17 cancer/testis antigens are candidate targets for immunotherapy of NSCLC and further suggest that multi-antigen vaccines may be beneficial.

  9. NY-ESO-1 is a sensitive and specific immunohistochemical marker for myxoid and round cell liposarcomas among related mesenchymal myxoid neoplasms.

    Science.gov (United States)

    Hemminger, Jessica A; Iwenofu, O Hans

    2013-09-01

    Myxoid and round cell liposarcomas constitute approximately one-third of all liposarcomas, a relatively common group of fat-derived soft tissue sarcomas. The histomorphology is a continuum between highly differentiated myxoid and poorly differentiated round cell components. The gold standard of diagnosis is dependent on histomorphology and/or identification of t(12;16)(q13;p11) translocation by cytogenetics or demonstration of DDIT3 rearrangements by fluorescence in situ hybridization. There are currently no diagnostic immunohistochemical stains available. The broad range of myxoid neoplasms in the differential diagnosis includes a variety of sarcomas. Given the notable differences in disease biology among myxoid neoplasms, which range from benign to aggressive, an accurate diagnosis is imperative for proper treatment and prognostication. Prompted by our recent study showing frequent expression of the cancer testis antigen NY-ESO-1 in myxoid and round cell liposarcomas, we sought to evaluate the utility of NY-ESO-1 as an immunohistochemical marker for myxoid and round cell liposarcoma among mesenchymal myxoid neoplasms within the differential diagnosis. Formalin-fixed, paraffin-embedded blocks were obtained for the following mesenchymal myxoid neoplasms (n=138): myxoid and round cell liposarcoma (n=38); well-differentiated liposarcoma (n=12); lipoma (n=20; 4 with myxoid change); extra-cardiac soft tissue myxoma (n=39); extraskeletal myxoid chondrosarcoma (n=12); myxofibrosarcoma (n=10: 5 low grade, 2 intermediate grade, 3 high grade); and low-grade fibromyxoid sarcoma (n=7). Utilizing standard immunohistochemistry protocols, full sections were stained with NY-ESO-1 (clone E978), and staining was assessed for intensity (1-2+), percentage of tumor positivity, and location. In all, 36/38 (95%) of the myxoid and round cell liposarcomas demonstrated NY-ESO-1 immunoreactivity. The majority of the positive cases (34/36; 94%) showed strong, homogenous staining (>50% tumor

  10. Expression of cancer/testis antigens in salivary gland carcinomas with reference to MAGE-A and NY-ESO-1 expression in adenoid cystic carcinoma.

    Science.gov (United States)

    Beppu, Shintaro; Ito, Yohei; Fujii, Kana; Saida, Kosuke; Takino, Hisashi; Masaki, Ayako; Murase, Takayuki; Kusafuka, Kimihide; Iida, Yoshiyuki; Onitsuka, Tetsuro; Yatabe, Yasushi; Hanai, Nobuhiro; Hasegawa, Yasuhisa; Ijichi, Kei; Murakami, Shingo; Inagaki, Hiroshi

    2017-08-01

    Cancer/testis antigens (CTAs) are detected in cancer cells but not in healthy normal tissues, with the exception of gametogenic tissues. CTAs are highly immunogenic proteins, and thus represent ideal targets for cytotoxic T-lymphocyte-mediated specific immune therapy. The aim of this study was to screen CTA expression in various types of salivary gland carcinoma and to clarify clinicopathological significance of MAGE-A and NY-ESO-1 expression in adenoid cystic carcinomas (AdCCs) of the salivary gland, which is one of the most common salivary gland carcinomas, and usually has a fatal outcome. We used immunohistochemistry to examine the expression of four CTAs (MAGE-A, NY-ESO-1, CT7, and GAGE7) in various types of salivary gland carcinoma (n = 95). When carcinoma cases were divided into low-grade and intermediate/high-grade types, NY-ESO-1 and CT7 were expressed more frequently in intermediate/high-grade carcinomas. We then focused on MAGE-A and NY-ESO-1 expression in a large cohort of adenoid cystic carcinomas (AdCCs) (n = 46). MAGE-A and NY-ESO-1 were frequently expressed in AdCC; specifically, MAGE-A was expressed in >60% of the AdCC cases. MAGE-A expression and tumour site (minor salivary gland) were identified as independent risk factors for locoregional tumour recurrence. These findings suggest that CTAs may be expressed in a variety of salivary gland carcinomas, especially in those with higher histological grades. In addition, MAGE-A, which is frequently expressed in AdCC cases, may be a useful prognostic factor for poorer locoregional recurrence-free survival. © 2017 John Wiley & Sons Ltd.

  11. Expression of MAGE-A and NY-ESO-1 cancer-testis antigens is enriched in triple-negative invasive breast cancers.

    Science.gov (United States)

    Raghavendra, Ashwini; Kalita-de Croft, Priyakshi; Vargas, Ana Cristina; Smart, Chanel E; Simpson, Peter T; Saunus, Jodi M; Lakhani, Sunil R

    2018-02-21

    A better understanding of the expression of cancer testis antigens (CTA) in breast cancer might identify new immunotherapy options, especially for triple-negative (TN) tumours, which lack expression of conventional therapeutic targets ER, PR and HER2 (receptors for Oestrogen, Progesterone and Human epidermal growth factor). The aim of this study was to quantify the expression of MAGE-A and NY-ESO-1 CTAs in breast cancer, and relate this to known clinicopathologic parameters. We surveyed MAGE-A and NY-ESO-1 protein expression in an unselected cohort of 367 breast tumours (out of which 65 tumours were TN), with accompanying clinical follow-up data, using immunohistochemistry (IHC) analysis of tissue microarrays. Relevant to their potential as vaccine targets in breast cancer, MAGE-A was expressed in 13% of cases, and NY-ESO-1 in 3.8%, with the majority of tumours exhibiting fairly homogeneous staining within individual tissue cores (~85% of cases with staining in >75% tumour cells). Most NY-ESO-1 positive cases also expressed MAGE-A (p=2.06x10 -9 ), and both were strongly associated with the TN phenotype (pESO-1 are frequently expressed in TNBC (~47% and 17% of TN cases, respectively), suggesting that targeting them could be feasible in this patient group. Expression is reasonably homogeneous in positive cases suggesting that IHC analysis of tissue biopsies would be a reliable companion biomarker. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  12. Bi-specific TCR-anti CD3 redirected T-cell targeting of NY-ESO-1- and LAGE-1-positive tumors.

    Science.gov (United States)

    McCormack, Emmet; Adams, Katherine J; Hassan, Namir J; Kotian, Akhil; Lissin, Nikolai M; Sami, Malkit; Mujić, Maja; Osdal, Tereza; Gjertsen, Bjørn Tore; Baker, Deborah; Powlesland, Alex S; Aleksic, Milos; Vuidepot, Annelise; Morteau, Olivier; Sutton, Deborah H; June, Carl H; Kalos, Michael; Ashfield, Rebecca; Jakobsen, Bent K

    2013-04-01

    NY-ESO-1 and LAGE-1 are cancer testis antigens with an ideal profile for tumor immunotherapy, combining up-regulation in many cancer types with highly restricted expression in normal tissues and sharing a common HLA-A*0201 epitope, 157-165. Here, we present data to describe the specificity and anti-tumor activity of a bifunctional ImmTAC, comprising a soluble, high-affinity T-cell receptor (TCR) specific for NY-ESO-1157-165 fused to an anti-CD3 scFv. This reagent, ImmTAC-NYE, is shown to kill HLA-A2, antigen-positive tumor cell lines, and freshly isolated HLA-A2- and LAGE-1-positive NSCLC cells. Employing time-domain optical imaging, we demonstrate in vivo targeting of fluorescently labelled high-affinity NYESO-specific TCRs to HLA-A2-, NY-ESO-1157-165-positive tumors in xenografted mice. In vivo ImmTAC-NYE efficacy was tested in a tumor model in which human lymphocytes were stably co-engrafted into NSG mice harboring tumor xenografts; efficacy was observed in both tumor prevention and established tumor models using a GFP fluorescence readout. Quantitative RT-PCR was used to analyze the expression of both NY-ESO-1 and LAGE-1 antigens in 15 normal tissues, 5 cancer cell lines, 10 NSCLC, and 10 ovarian cancer samples. Overall, LAGE-1 RNA was expressed at a greater frequency and at higher levels than NY-ESO-1 in the tumor samples. These data support the clinical utility of ImmTAC-NYE as an immunotherapeutic agent for a variety of cancers.

  13. The most metal-poor Galactic globular cluster: the first spectroscopic observations of ESO280-SC06

    Science.gov (United States)

    Simpson, Jeffrey D.

    2018-04-01

    We present the first spectroscopic observations of the very metal-poor Milky Way globular cluster ESO280-SC06. Using spectra acquired with the 2dF/AAOmega spectrograph on the Anglo-Australian Telescope, we have identified 13 members of the cluster, and estimate from their infrared calcium triplet lines that the cluster has a metallicity of [Fe/H]={-2.48}^{+0.06}_{-0.11}. This would make it the most metal-poor globular cluster known in the Milky Way. This result was verified with comparisons to three other metal-poor globular clusters that had been observed and analyzed in the same manner. We also present new photometry of the cluster from EFOSC2 and SkyMapper and confirm that the cluster is located 22.9 ± 2.1 kpc from the Sun and 15.2 ± 2.1 kpc from the Galactic centre, and has a radial velocity of 92.5 + 2.4-1.6 km s-1. These new data finds the cluster to have a radius about half that previously estimated, and we find that the cluster has a dynamical mass of the cluster of (12 ± 2) × 103 M⊙. Unfortunately, we lack reliable proper motions to fully characterize its orbit about the Galaxy. Intriguingly, the photometry suggests that the cluster lacks a well-populated horizontal branch, something that has not been observed in a cluster so ancient or metal-poor.

  14. La competencia sobre planificación de investigaciones en 4º de ESO: un estudio de caso

    Directory of Open Access Journals (Sweden)

    Iñigo RODRÍGUEZ ARTECHE

    2016-01-01

    Full Text Available En los últimos años, las metodologías de tipo “Inquiry-Based Science Education” (IBSE se vienen recomendando desde las instancias europeas para mejorar los niveles de competencia y las actitudes hacia la ciencia de los estudiantes. Así, el presente estudio pretende comprobar la eficacia de una metodología IBSE, la Metodología de Resolución de Problemas como Investigación (MRPI, para la enseñanza-aprendizaje de la competencia sobre planificación de investigaciones en 4º de ESO. Para ello, a partir de las resoluciones de problemas abiertos de los estudiantes, se analiza el progreso en el aprendizaje de las dimensiones competenciales relativas a la planificación de investigaciones y la influencia de la metodología propuesta en su adquisición. Los resultados indican un dominio de mayor a menor grado en la emisión de hipótesis, diseño de la experimentación, representación cualitativa, identificación de variables y reformulación del problema abierto, así como la influencia positiva de la MRPI en estas dimensiones competenciales sobre la planificación de investigaciones.

  15. UNA PROPUESTA PARA LA MEJORA DE LA ADECUACIÓN EN LA EXPRESIÓN ESCRITA EN LA E.S.O.

    Directory of Open Access Journals (Sweden)

    Mª del Pilar Mesa Arroyo

    2012-01-01

    Full Text Available Partiendo de los malos resultados obtenidos por el alumnado andaluz en competencia en comunicación lingüística en las pruebas de diagnóstico efectuadas por la Consejería de Educación de la Junta de Andalucía, y teniendo en cuenta la escasa presencia de actividades de expresión escrita adecuadas en los libros de texto utilizados actualmente en los institutos de educación secundaria de Andalucía; planteamos una propuesta didáctica que, basada en el análisis microfuncional, pretende la mejora de la adecuación de los textos compuestos por alumnado de la ESO. La efectividad de nuestra propuesta se ha comprobado mediante la evaluación de textos compuestos por 40 alumnos de 3º de ESO de dos centros de secundaria andaluces.

  16. Non-classical antigen processing pathways are required for MHC class II-restricted direct tumor recognition by NY-ESO-1-specific CD4+ T cells

    Science.gov (United States)

    Matsuzaki, Junko; Tsuji, Takemasa; Luescher, Immanuel; Old, Lloyd J.; Shrikant, Protul; Gnjatic, Sacha; Odunsi, Kunle

    2014-01-01

    Tumor antigen-specific CD4+ T cells that directly recognize cancer cells are important for orchestrating antitumor immune responses at the local tumor sites. However, the mechanisms of direct MHC class II (MHC-II) presentation of intracellular tumor antigen by cancer cells are poorly understood. We found that two functionally distinct subsets of CD4+ T cells were expanded after HLA-DPB1*04 (DP04)-binding NY-ESO-1157–170 peptide vaccination in ovarian cancer patients. While both subsets similarly recognized exogenous NY-ESO-1 protein pulsed on DP04+ target cells, only one type recognized target cells with intracellular expression of NY-ESO-1. The tumor-recognizing CD4+ T cells more efficiently recognized the short 8–9-mer peptides than the non-tumor-recognizing CD4+ T cells. In addition to endosomal/lysosomal proteases that are typically involved in MHC-II antigen presentation, several pathways in the MHC class I presentation pathways such as the proteasomal degradation and transporter-associated with antigen-processing (TAP)-mediated peptide transport were also involved in the presentation of intracellular NY-ESO-1 on MHC-II. The presentation was inhibited significantly by primaquine, a small molecule that inhibits endosomal recycling, consistent with findings that pharmacological inhibition of new protein synthesis enhances antigen presentation. Together, our data demonstrated that cancer cells selectively present peptides from intracellular tumor antigens on MHC-II by multiple non-classical antigen-processing pathways. Harnessing direct tumor-recognizing ability of CD4+ T cells could be a promising strategy to enhance antitumor immune responses in the immunosuppressive tumor microenvironment. PMID:24764581

  17. Nonclassical antigen-processing pathways are required for MHC class II-restricted direct tumor recognition by NY-ESO-1-specific CD4(+) T cells.

    Science.gov (United States)

    Matsuzaki, Junko; Tsuji, Takemasa; Luescher, Immanuel; Old, Lloyd J; Shrikant, Protul; Gnjatic, Sacha; Odunsi, Kunle

    2014-04-01

    Tumor antigen-specific CD4(+) T cells that directly recognize cancer cells are important for orchestrating antitumor immune responses at the local tumor sites. However, the mechanisms of direct MHC class II (MHC-II) presentation of intracellular tumor antigen by cancer cells are poorly understood. We found that two functionally distinct subsets of CD4(+) T cells were expanded after HLA-DPB1*04 (DP04)-binding NY-ESO-1157-170 peptide vaccination in patients with ovarian cancer. Although both subsets recognized exogenous NY-ESO-1 protein pulsed on DP04(+) target cells, only one type recognized target cells with intracellular expression of NY-ESO-1. The tumor-recognizing CD4(+) T cells more efficiently recognized the short 8-9-mer peptides than the non-tumor-recognizing CD4(+) T cells. In addition to endosomal/lysosomal proteases that are typically involved in MHC-II antigen presentation, several pathways in the MHC class I presentation pathways, such as the proteasomal degradation and transporter-associated with antigen-processing-mediated peptide transport, were also involved in the presentation of intracellular NY-ESO-1 on MHC-II. The presentation was inhibited significantly by primaquine, a small molecule that inhibits endosomal recycling, consistent with findings that pharmacologic inhibition of new protein synthesis enhances antigen presentation. Together, our data demonstrate that cancer cells selectively present peptides from intracellular tumor antigens on MHC-II by multiple nonclassical antigen-processing pathways. Harnessing the direct tumor-recognizing ability of CD4(+) T cells could be a promising strategy to enhance antitumor immune responses in the immunosuppressive tumor microenvironment.

  18. The Gaia-ESO Survey: the present-day radial metallicity distribution of the Galactic disc probed by pre-main-sequence clusters

    Science.gov (United States)

    Spina, L.; Randich, S.; Magrini, L.; Jeffries, R. D.; Friel, E. D.; Sacco, G. G.; Pancino, E.; Bonito, R.; Bravi, L.; Franciosini, E.; Klutsch, A.; Montes, D.; Gilmore, G.; Vallenari, A.; Bensby, T.; Bragaglia, A.; Flaccomio, E.; Koposov, S. E.; Korn, A. J.; Lanzafame, A. C.; Smiljanic, R.; Bayo, A.; Carraro, G.; Casey, A. R.; Costado, M. T.; Damiani, F.; Donati, P.; Frasca, A.; Hourihane, A.; Jofré, P.; Lewis, J.; Lind, K.; Monaco, L.; Morbidelli, L.; Prisinzano, L.; Sousa, S. G.; Worley, C. C.; Zaggia, S.

    2017-05-01

    Context. The radial metallicity distribution in the Galactic thin disc represents a crucial constraint for modelling disc formation and evolution. Open star clusters allow us to derive both the radial metallicity distribution and its evolution over time. Aims: In this paper we perform the first investigation of the present-day radial metallicity distribution based on [Fe/H] determinations in late type members of pre-main-sequence clusters. Because of their youth, these clusters are therefore essential for tracing the current interstellar medium metallicity. Methods: We used the products of the Gaia-ESO Survey analysis of 12 young regions (age ages is not easily explained by the models. Our results reveal a complex interplay of several processes (e.g. star formation activity, initial mass function, supernova yields, gas flows) that controlled the recent evolution of the Milky Way. Based on observations made with the ESO/VLT, at Paranal Observatory, under program 188.B-3002 (The Gaia-ESO Public Spectroscopic Survey).Full Table 1 is only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/601/A70

  19. Optical Detectors for Astronomy II : State-of-the-art at the Turn of the Millennium : 4th ESO CCD Workshop

    CERN Document Server

    Beletic, James

    2000-01-01

    th The 4 ESO CCO Workshop, Optical Detectors for Astronomy, was held during September 13-16, 1999 at its usual location, the headquarters of the European Southern Observatory in Garching, Germany. We prefer to remember this workshop as a "meeting of friends", who came to Garching to visit ESO and to present their work, rather than a formal meeting. Based on our experience with the 1996 ESO CCO workshop, we deliberately put emphasis on creating an environment that encouraged the participants to stay together and informally exchange ideas. These informal events began with a tour of the BWM auto factory and continued with a reception at "SchloB Beletic", the conference dinner at a real SchloB of the Bavarian International School (where the participants enjoyed basket, baseball, table soccer, rock climbing and eventually dancing) and concluded with a tour of the Paulaner Brewery and dinner at the Seehaus in the Englisher Garten. The lunch "Biergarten", adjacent to the poster session area, was a daily meeting poin...

  20. Chemical synthesis of a polypeptide backbone derived from the primary sequence of the cancer protein NY-ESO-1 enabled by kinetically controlled ligation and pseudoprolines.

    Science.gov (United States)

    Harris, Paul W R; Brimble, Margaret A

    2015-03-01

    The cancer protein NY-ESO-1 has been shown to be one of the most promising vaccine candidates although little is known about its cellular function. Using a chemical protein strategy, the 180 amino acid polypeptide, tagged with an arginine solubilizing tail, was assembled in a convergent manner from four unprotected peptide α-thioester peptide building blocks and one cysteinyl polypeptide, which were in turn prepared by Boc and Fmoc solid phase peptide synthesis (SPPS) respectively. To facilitate the assembly by ligation chemistries, non-native cysteines were introduced as chemical handles into the polypeptide fragments; pseudoproline dipeptides and microwave assisted Fmoc SPPS were crucial techniques to prepare the challenging hydrophobic C-terminal fragment. Three sequential kinetically controlled ligations, which exploited the reactivity between peptide arylthioesters and peptide alkylthioesters, were then used in order to assemble the more tractable N-terminal region of NY-ESO-1. The ensuing 147 residue polypeptide thioester then underwent successful final native chemical ligation with the very hydrophobic C-terminal polypeptide bearing an N-terminal cysteine affording the 186 residue polypeptide as an advanced intermediate en route to the native NY-ESO-1 protein. © 2015 Wiley Periodicals, Inc.

  1. SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade.

    Science.gov (United States)

    Xue, Wei; Metheringham, Rachael L; Brentville, Victoria A; Gunn, Barbara; Symonds, Peter; Yagita, Hideo; Ramage, Judith M; Durrant, Lindy G

    2016-06-01

    Checkpoint blockade has demonstrated promising antitumor responses in approximately 10-40% of patients. However, the majority of patients do not make a productive immune response to their tumors and do not respond to checkpoint blockade. These patients may benefit from an effective vaccine that stimulates high-avidity T cell responses in combination with checkpoint blockade. We have previously shown that incorporating TRP-2 and gp100 epitopes into the CDR regions of a human IgG1 DNA (ImmunoBody®: IB) results in significant tumor regression both in animal models and patients. This vaccination strategy is superior to others as it targets antigen to antigen-presenting cells and stimulates high-avidity T cell responses. To broaden the application of this vaccination strategy, 16 NY-ESO-1 epitopes, covering over 80% of HLA phenotypes, were incorporated into the IB (SCIB2). They produced higher frequency and avidity T cell responses than peptide vaccination. These T cells were of sufficient avidity to kill NY-ESO-1-expressing tumor cells, and in vivo controlled the growth of established B16-NY-ESO-1 tumors, resulting in long-term survival (35%). When SCIB2 was given in combination with Treg depletion, CTLA-4 blockade or PD-1 blockade, long-term survival from established tumors was significantly enhanced to 56, 67 and 100%, respectively. Translating these responses into the clinic by using a combination of SCIB2 vaccination and checkpoint blockade can only further improve clinical responses.

  2. Detection of the dusty torus in AGN with COMIC, the new infrared camera dedicated to the ESO adaptive optics system.

    Science.gov (United States)

    Marco, Olivier

    1997-10-01

    High angular resolution observation has greatly benefitted from adaptive optic systems working in the infrared. The COMIC camera, the second camera dedicated to ADONIS, the ESO 3.60 meter telescope adaptive optics system, allows observation in the spectral range 3-5 micron at the diffraction limit of the telescope. The characterization of the camera at the Meudon and Grenoble laboratories, then its performances determination on the sky in Chile constitute the first part of this dissertation. A new method for evaluating the limiting magnitudes is proposed which takes into account the various contributions to the detectivity loss between laboratory testing and real condition observations. This approach can be transferred to any other case where a priori observing conditions are known ( Strehl ratio or seeing ). Study of the central region of Active Galactic Nuclei (~1 arcsec) requires high angular resolution. In particular, warm and hot dust is emissive in the 1-5 micron spectral region. Thus, adaptive optics observations are well suited to AGN observation. It is predicted that the central engine and its neighboring environment are embedded within an optically thin dusty/molecular torus which may, along some lines of sight, obscure and even fully hide the nuclear emission leading to distinct observational properties for objects supposed to be identical. Observations made with ADONIS & COMIC of NGC7469 and NGC1068 have shown large amounts of dust located in the torus but also mixed with gas from the narrow line emission region. The high angular resolution achieved has allowed the determination of the dust temperature, mass and spatial distribution around the AGN central engine. These results are in agreement with several torus models and could help to constrain them.

  3. Trypanosoma cruzi Adjuvants Potentiate T Cell-Mediated Immunity Induced by a NY-ESO-1 Based Antitumor Vaccine

    Science.gov (United States)

    Junqueira, Caroline; Guerrero, Ana Tereza; Galvão-Filho, Bruno; Andrade, Warrison A.; Salgado, Ana Paula C.; Cunha, Thiago M.; Ropert, Catherine; Campos, Marco Antônio; Penido, Marcus L. O.; Mendonça-Previato, Lúcia; Previato, José Oswaldo; Ritter, Gerd; Cunha, Fernando Q.; Gazzinelli, Ricardo T.

    2012-01-01

    Immunological adjuvants that induce T cell-mediate immunity (TCMI) with the least side effects are needed for the development of human vaccines. Glycoinositolphospholipids (GIPL) and CpGs oligodeoxynucleotides (CpG ODNs) derived from the protozoa parasite Trypanosoma cruzi induce potent pro-inflammatory reaction through activation of Toll-Like Receptor (TLR)4 and TLR9, respectively. Here, using mouse models, we tested the T. cruzi derived TLR agonists as immunological adjuvants in an antitumor vaccine. For comparison, we used well-established TLR agonists, such as the bacterial derived monophosphoryl lipid A (MPL), lipopeptide (Pam3Cys), and CpG ODN. All tested TLR agonists were comparable to induce antibody responses, whereas significant differences were noticed in their ability to elicit CD4+ T and CD8+ T cell responses. In particular, both GIPLs (GTH, and GY) and CpG ODNs (B344, B297 and B128) derived from T. cruzi elicited interferon-gamma (IFN-γ) production by CD4+ T cells. On the other hand, the parasite derived CpG ODNs, but not GIPLs, elicited a potent IFN-γ response by CD8+ T lymphocytes. The side effects were also evaluated by local pain (hypernociception). The intensity of hypernociception induced by vaccination was alleviated by administration of an analgesic drug without affecting protective immunity. Finally, the level of protective immunity against the NY-ESO-1 expressing melanoma was associated with the magnitude of both CD4+ T and CD8+ T cell responses elicited by a specific immunological adjuvant. PMID:22567144

  4. The ESO Diffuse Interstellar Bands Large Exploration Survey (EDIBLES) . I. Project description, survey sample, and quality assessment

    Science.gov (United States)

    Cox, Nick L. J.; Cami, Jan; Farhang, Amin; Smoker, Jonathan; Monreal-Ibero, Ana; Lallement, Rosine; Sarre, Peter J.; Marshall, Charlotte C. M.; Smith, Keith T.; Evans, Christopher J.; Royer, Pierre; Linnartz, Harold; Cordiner, Martin A.; Joblin, Christine; van Loon, Jacco Th.; Foing, Bernard H.; Bhatt, Neil H.; Bron, Emeric; Elyajouri, Meriem; de Koter, Alex; Ehrenfreund, Pascale; Javadi, Atefeh; Kaper, Lex; Khosroshadi, Habib G.; Laverick, Mike; Le Petit, Franck; Mulas, Giacomo; Roueff, Evelyne; Salama, Farid; Spaans, Marco

    2017-10-01

    The carriers of the diffuse interstellar bands (DIBs) are largely unidentified molecules ubiquitously present in the interstellar medium (ISM). After decades of study, two strong and possibly three weak near-infrared DIBs have recently been attributed to the C60^+ fullerene based on observational and laboratory measurements. There is great promise for the identification of the over 400 other known DIBs, as this result could provide chemical hints towards other possible carriers. In an effort tosystematically study the properties of the DIB carriers, we have initiated a new large-scale observational survey: the ESO Diffuse Interstellar Bands Large Exploration Survey (EDIBLES). The main objective is to build on and extend existing DIB surveys to make a major step forward in characterising the physical and chemical conditions for a statistically significant sample of interstellar lines-of-sight, with the goal to reverse-engineer key molecular properties of the DIB carriers. EDIBLES is a filler Large Programme using the Ultraviolet and Visual Echelle Spectrograph at the Very Large Telescope at Paranal, Chile. It is designed to provide an observationally unbiased view of the presence and behaviour of the DIBs towards early-spectral-type stars whose lines-of-sight probe the diffuse-to-translucent ISM. Such a complete dataset will provide a deep census of the atomic and molecular content, physical conditions, chemical abundances and elemental depletion levels for each sightline. Achieving these goals requires a homogeneous set of high-quality data in terms of resolution (R 70 000-100 000), sensitivity (S/N up to 1000 per resolution element), and spectral coverage (305-1042 nm), as well as a large sample size (100+ sightlines). In this first paper the goals, objectives and methodology of the EDIBLES programme are described and an initial assessment of the data is provided.

  5. The ESO Diffuse Interstellar Bands Large Exploration Survey: EDIBLES I. Project description, survey sample and quality assessment.

    Science.gov (United States)

    Cox, Nick L J; Cami, Jan; Farhang, Amin; Smoker, Jonathan; Monreal-Ibero, Ana; Lallement, Rosine; Sarre, Peter J; Marshall, Charlotte C M; Smith, Keith T; Evans, Christopher J; Royer, Pierre; Linnartz, Harold; Cordiner, Martin A; Joblin, Christine; van Loon, Jacco Th; Foing, Bernard H; Bhatt, Neil H; Bron, Emeric; Elyajouri, Meriem; de Koter, Alex; Ehrenfreund, Pascale; Javadi, Atefeh; Kaper, Lex; Khosroshadi, Habib G; Laverick, Mike; Le Petit, Franck; Mulas, Giacomo; Roueff, Evelyne; Salama, Farid; Spaans, Marco

    2017-10-01

    The carriers of the diffuse interstellar bands (DIBs) are largely unidentified molecules ubiquitously present in the interstellar medium (ISM). After decades of study, two strong and possibly three weak near-infrared DIBs have recently been attributed to the [Formula: see text] fullerene based on observational and laboratory measurements. There is great promise for the identification of the over 400 other known DIBs, as this result could provide chemical hints towards other possible carriers. In an effort to systematically study the properties of the DIB carriers, we have initiated a new large-scale observational survey: the ESO Diffuse Interstellar Bands Large Exploration Survey (EDIBLES). The main objective is to build on and extend existing DIB surveys to make a major step forward in characterising the physical and chemical conditions for a statistically significant sample of interstellar lines-of-sight, with the goal to reverse-engineer key molecular properties of the DIB carriers. EDIBLES is a filler Large Programme using the Ultraviolet and Visual Echelle Spectrograph at the Very Large Telescope at Paranal, Chile. It is designed to provide an observationally unbiased view of the presence and behaviour of the DIBs towards early-spectral type stars whose lines-of-sight probe the diffuse-to-translucent ISM. Such a complete dataset will provide a deep census of the atomic and molecular content, physical conditions, chemical abundances and elemental depletion levels for each sightline. Achieving these goals requires a homogeneous set of high-quality data in terms of resolution ( R ~ 70 000 - 100 000), sensitivity (S/N up to 1000 per resolution element), and spectral coverage (305-1042 nm), as well as a large sample size (100+ sightlines). In this first paper the goals, objectives and methodology of the EDIBLES programme are described and an initial assessment of the data is provided.

  6. Football as a tool for the job of the values and attitudes in the ESO according to the Basic Competencies

    Directory of Open Access Journals (Sweden)

    David Rodríguez Ruiz

    2010-01-01

    Full Text Available In this study we present a practical experience conducted in the I.E.S. José Arencibia Gil (Telde – Gran Canaria with students from 3º ESO, based on the development of basic competencies. The aim is to work «values and attitudes» from the context that traces the BOC Decree 127/2007 of June 2007, using football as a tool, because the role of «integral education» which Physical Education holds. To assess the achievement of the objectives was made for a simplified form of the work presented by Gil et al. (2006 in its proposition of values and indicators to evaluate in games and sports and to assess the level of awareness and achievement of the objectives and the level of involvement in work rose from a student questionnaire. With the result over 96% of students having fun, strive to maintain a coherent social relationships, play fair, emotionally controlled and respect the rules, on the other hand, that the total number of students included in the classes (n=118 an 80% of them had understood the purpose of the sessions. 49 students thought that it was intended to «learn how to interact» and 45 «to improve the skills and abilities». The results lead us to conclude that the basic competencies «strengthen and give greater importance to the integrative role of Physical Education» and it shows as «pillar of education in values and attitudes without releasing their specifics contents and objectives».

  7. Antibody-targeted NY-ESO-1 to mannose receptor or DEC-205 in vitro elicits dual human CD8+ and CD4+ T cell responses with broad antigen specificity.

    Science.gov (United States)

    Tsuji, Takemasa; Matsuzaki, Junko; Kelly, Marcus P; Ramakrishna, Venky; Vitale, Laura; He, Li-Zhen; Keler, Tibor; Odunsi, Kunle; Old, Lloyd J; Ritter, Gerd; Gnjatic, Sacha

    2011-01-15

    Immunization of cancer patients with vaccines containing full-length tumor Ags aims to elicit specific Abs and both CD4(+) and CD8(+) T cells. Vaccination with protein Ags, however, often elicits only CD4(+) T cell responses without inducing Ag-specific CD8(+) T cells, as exogenous protein is primarily presented to CD4(+) T cells. Recent data revealed that Ab-mediated targeting of protein Ags to cell surface receptors on dendritic cells could enhance the induction of both CD4(+) and CD8(+) T cells. We investigated in this study if these observations were applicable to NY-ESO-1, a cancer-testis Ag widely used in clinical cancer vaccine trials. We generated two novel targeting proteins consisting of the full-length NY-ESO-1 fused to the C terminus of two human mAbs against the human mannose receptor and DEC-205, both internalizing molecules expressed on APC. These targeting proteins were evaluated for their ability to activate NY-ESO-1-specific human CD4(+) and CD8(+) T cells in vitro. Both targeted NY-ESO-1 proteins rapidly bound to their respective targets on APC. Whereas nontargeted and Ab-targeted NY-ESO-1 proteins similarly activated CD4(+) T cells, cross-presentation to CD8(+) T cells was only efficiently induced by targeted NY-ESO-1. In addition, both mannose receptor and DEC-205 targeting elicited specific CD4(+) and CD8(+) T cells from PBLs of cancer patients. Receptor-specific delivery of NY-ESO-1 to APC appears to be a promising vaccination strategy to efficiently generate integrated and broad Ag-specific immune responses against NY-ESO-1 in cancer patients.

  8. SEARCHES FOR METAL-POOR STARS FROM THE HAMBURG/ESO SURVEY USING THE CH G BAND

    Energy Technology Data Exchange (ETDEWEB)

    Placco, Vinicius M.; Rossi, Silvia [Departamento de Astronomia-Instituto de Astronomia, Geofisica e Ciencias Atmosfericas, Universidade de Sao Paulo, Sao Paulo, SP 05508-090 (Brazil); Kennedy, Catherine R.; Beers, Timothy C.; Lee, Young Sun [Department of Physics and Astronomy and JINA (Joint Institute for Nuclear Astrophysics), Michigan State University, East Lansing, MI 48824 (United States); Christlieb, Norbert [Zentrum fuer Astronomie der Universitaet Heidelberg, Landessternwarte, Koenigstuhl 12, 69117 Heidelberg (Germany); Sivarani, Thirupathi [Indian Institute of Astrophysics, 2nd Block, Koramangala, Bangalore 560034 (India); Reimers, Dieter [Hamburger Sternwarte, Universitaet Hamburg, Gojenbergsweg 112, 21029 Hamburg (Germany); Wisotzki, Lutz, E-mail: vmplacco@astro.iag.usp.br [Astrophysical Institute Potsdam, An der Sternwarte 16, 14482 Potsdam (Germany)

    2011-12-15

    We describe a new method to search for metal-poor candidates from the Hamburg/ESO objective-prism survey (HES) based on identifying stars with apparently strong CH G-band strengths for their colors. The hypothesis we exploit is that large overabundances of carbon are common among metal-poor stars, as has been found by numerous studies over the past two decades. The selection was made by considering two line indices in the 4300 A region, applied directly to the low-resolution prism spectra. This work also extends a previously published method by adding bright sources to the sample. The spectra of these stars suffer from saturation effects, compromising the index calculations and leading to an undersampling of the brighter candidates. A simple numerical procedure, based on available photometry, was developed to correct the line indices and overcome this limitation. Visual inspection and classification of the spectra from the HES plates yielded a list of 5288 new metal-poor (and by selection, carbon-rich) candidates, which are presently being used as targets for medium-resolution spectroscopic follow-up. Estimates of the stellar atmospheric parameters, as well as carbon abundances, are now available for 117 of the first candidates, based on follow-up medium-resolution spectra obtained with the SOAR 4.1 m and Gemini 8 m telescopes. We demonstrate that our new method improves the metal-poor star fractions found by our pilot study by up to a factor of three in the same magnitude range, as compared with our pilot study based on only one CH G-band index. Our selection scheme obtained roughly a 40% success rate for identification of stars with [Fe/H] <-1.0; the primary contaminant is late-type stars with near-solar abundances and, often, emission line cores that filled in the Ca II K line on the prism spectrum. Because the selection is based on carbon, we greatly increase the numbers of known carbon-enhanced metal-poor stars from the HES with intermediate metallicities -2

  9. New high-sensitivity, milliarcsecond resolution results from routine observations of lunar occultations at the ESO VLT

    Science.gov (United States)

    Richichi, A.; Fors, O.; Chen, W.-P.; Mason, E.

    2010-11-01

    for the first time. We have established upper limits on the angular size of 177 stars, mostly in the 1 to 5 mas range, and we plan to include them in a future list of sources well suited for the calibration of interferometers. Conclusions: We confirm the performance of the technique already established in our previous work. LO at an 8 m-class telescope can achieve an angular resolution close to 0farcs001 with a sensitivity K ≈ 12 mag. Based on observations made with ESO telescopes at Paranal Observatory.

  10. LA EDUCACIÓN PARA LA SALUD EN LA ESO: APORTACIONES DE UN ESTUDIO SOBRE EL PAÍS VASCO

    Directory of Open Access Journals (Sweden)

    Amando Vega Fuente

    2015-01-01

    Full Text Available La educación para la salud en las escuelas es una estrategia necesaria para favorecer comportamientos saludables en el alumnado. Este estudio tiene como objetivo disponer de un conocimiento más preciso sobre las conductas de riesgo de los adolescentes y sobre el tratamiento de las cuestiones de salud en los centros de educación secundaria obligatoria. La prevención del consumo de drogas, la educación afectivo-sexual, la formación para la seguridad vial, la promoción de la salud física y mental son algunos de los aspectos clave para el desarrollo integral de los adolescentes. El trabajo de investigación se llevó a cabo en 24 institutos de ESO del País Vasco y han tomado parte 571 estudiantes. Todos ellos han participado en los programas de educación para la salud subvencionados por el Departamento de Educación del Gobierno Vasco durante los cursos 2010-11 y 2011-12. Los datos obtenidos mediante cuestionarios fueron tratados con el paquete informático SPSS 19.0 e ITEMAN realizándose diversos análisis estadísticos (porcentajes y correlaciones de Pearson. Los resultados obtenidos muestran que las conductas no saludables de los alumnos y alumnas no sólo están relacionadas entre sí, sino también con su bienestar personal y escolar. Se constata, por otra parte, una necesidad clara de que los centros escolares asuman un mayor compromiso con la educación y la promoción de la salud en sus proyectos educativos. Se echa en falta la integración de las cuestiones de salud en los proyectos de los centros, por razones varias, como la falta de una política comprometida con este ámbito educativo, las carencias de formación de los profesores y profesoras y posiblemente la insuficiencia de los recursos dedicados en los centros a la práctica educativa en temas de salud.

  11. The ROSAT-ESO flux limited X-ray (REFLEX) galaxy cluster survey. I. The construction of the cluster sample

    Science.gov (United States)

    Böhringer, H.; Schuecker, P.; Guzzo, L.; Collins, C. A.; Voges, W.; Schindler, S.; Neumann, D. M.; Cruddace, R. G.; De Grandi, S.; Chincarini, G.; Edge, A. C.; MacGillivray, H. T.; Shaver, P.

    2001-04-01

    We discuss the construction of an X-ray flux-limited sample of galaxy clusters, the REFLEX survey catalogue, to be used for cosmological studies. This cluster identification and redshift survey was conducted in the frame of an ESO key programme and is based on candidates selected from the southern part of the ROSAT All-Sky Survey (RASS). For the first cluster candidate selection from a flux-limited RASS source list, we make use of optical data from the COSMOS digital catalogue produced from the scans of the UK-Schmidt plates. To ensure homogeneity of the sample construction process, this selection is based only on this one well-defined optical data base. The nature of the candidates selected in this process is subsequently checked by a more detailed evaluation of the X-ray and optical source properties and available literature data. The final identification and the redshift is then based on optical spectroscopic follow-up observations. In this paper we document the process by which the primary cluster candidate catalogue is constructed prior to the optical follow-up observations. We describe the reanalysis of the RASS source catalogue which enables us to impose a proper flux limit cut to the X-ray source list without introducing a severe bias against extended sources. We discuss the correlation of the X-ray and optical (COSMOS) data to find galaxy density enhancements at the RASS X-ray source positions and the further evaluation of the nature of these cluster candidates. Based also on the results of the follow-up observations we provide a statistical analysis of the completeness and contamination of the final cluster sample and show results on the cluster number counts. The final sample of identified X-ray clusters reaches a flux limit of 3 10-12 erg s-1 cm-2 in the 0.1-2.4 keV band and comprises 452 clusters in an area of 4.24 ster. The results imply a completeness of the REFLEX cluster sample well in excess of 90%. We also derive for the first time an upper limit

  12. Searches for Metal-poor Stars from the Hamburg/ESO Survey Using the CH G Band

    Science.gov (United States)

    Placco, Vinicius M.; Kennedy, Catherine R.; Beers, Timothy C.; Christlieb, Norbert; Rossi, Silvia; Sivarani, Thirupathi; Lee, Young Sun; Reimers, Dieter; Wisotzki, Lutz

    2011-12-01

    We describe a new method to search for metal-poor candidates from the Hamburg/ESO objective-prism survey (HES) based on identifying stars with apparently strong CH G-band strengths for their colors. The hypothesis we exploit is that large overabundances of carbon are common among metal-poor stars, as has been found by numerous studies over the past two decades. The selection was made by considering two line indices in the 4300 Å region, applied directly to the low-resolution prism spectra. This work also extends a previously published method by adding bright sources to the sample. The spectra of these stars suffer from saturation effects, compromising the index calculations and leading to an undersampling of the brighter candidates. A simple numerical procedure, based on available photometry, was developed to correct the line indices and overcome this limitation. Visual inspection and classification of the spectra from the HES plates yielded a list of 5288 new metal-poor (and by selection, carbon-rich) candidates, which are presently being used as targets for medium-resolution spectroscopic follow-up. Estimates of the stellar atmospheric parameters, as well as carbon abundances, are now available for 117 of the first candidates, based on follow-up medium-resolution spectra obtained with the SOAR 4.1 m and Gemini 8 m telescopes. We demonstrate that our new method improves the metal-poor star fractions found by our pilot study by up to a factor of three in the same magnitude range, as compared with our pilot study based on only one CH G-band index. Our selection scheme obtained roughly a 40% success rate for identification of stars with [Fe/H] contaminant is late-type stars with near-solar abundances and, often, emission line cores that filled in the Ca II K line on the prism spectrum. Because the selection is based on carbon, we greatly increase the numbers of known carbon-enhanced metal-poor stars from the HES with intermediate metallicities -2.0 < [Fe/H] <-1

  13. Effective inhibition of melanoma tumorigenesis and growth via a new complex vaccine based on NY-ESO-1-alum-polysaccharide-HH2

    Science.gov (United States)

    2014-01-01

    Background A safe and effective adjuvant plays an important role in the development of a vaccine. However, adjuvants licensed for administration in humans remain limited. Here, for the first time, we developed a novel combination adjuvant alum-polysaccharide-HH2 (APH) with potent immunomodulating activities, consisting of alum, polysaccharide of Escherichia coli and the synthetic cationic innate defense regulator peptide HH2. Methods The adjuvant effects of APH were examined using NY-ESO-1 protein-based vaccines in prophylactic and therapeutic models. We further determined the immunogenicity and anti-tumor effect of NY-ESO-1-APH (NAPH) vaccine using adoptive cellular/serum therapy in C57/B6 and nude mice. Cell-mediated and antibody-mediated immune responses were evaluated. Results The APH complex significantly promoted antigen uptake, maturation and cross-presentation of dendritic cells and enhanced the secretion of TNF-α, MCP-1 and IFN-γ by human peripheral blood mononuclear cells compared with individual components. Vaccination of NAPH resulted in significant tumor regression or delayed tumor progression in prophylactic and therapeutic models. In addition, passive serum/cellular therapy potently inhibited tumor growth of NY-ESO-1-B16. Mice treated with NAPH vaccine produced higher antibody titers and greater antibody-dependent/independent cellular cytotoxicity. Therefore, NAPH vaccination effectively stimulated innate immunity, and boosted both arms of the adaptive humoral and cellular immune responses to suppress tumorigenesis and growth of melanoma. Conclusions Our study revealed the potential application of APH complex as a novel immunomodulatory agent for vaccines against tumor refractory and growth. PMID:25070035

  14. Influencia de las creencias de las relaciones de pareja sobre la violencia de género, en el alumnado de un grupo de la ESO

    OpenAIRE

    Ojeda-Macía, Jone

    2013-01-01

    El presente trabajo de investigación tiene por objeto identificar las creencias que pueden tener los adolescentes, con respecto a las relaciones de pareja y valorar cómo éstas pueden estar relacionadas con la violencia de género. Para ello se utiliza una metodología descriptiva de estudio de casos, que se aplica a una muestra de 22 alumnos y alumnas de 4º de la ESO del Colegio María Auxiliadora de Vigo. La actual investigación confirma la existencia de creencias irracionales so...

  15. Dificultades en el proceso de la enseñanza-aprendizaje de la geometría en 1º de la ESO

    OpenAIRE

    Laliena-Tolosana, Francisco Javier

    2013-01-01

    El trabajo que se presenta a continuación, trata sobre las dificultades que surgen en el proceso de enseñanza-aprendizaje de la geometría en 1º de la ESO. En primer lugar, se analiza mediante un trabajo de campo la situación real de una clase de dicho curso, referida tanto a sus conocimientos y dificultades en este área como a sus circunstancias personales. Una vez conocida la situación, se buscan soluciones que mejoren estas dificultades. La búsqueda de estas soluciones se realiza en el camp...

  16. Enseñanza de longitudes y áreas en 1º ESO, mediante el aprendizaje cooperativo y aprendizaje basado en problemas

    OpenAIRE

    Alcañiz-Herreros, Jose Fº

    2017-01-01

    Geometry is the essential base for social, cultural and academic studies. As it can be found all around us, it can easily be connected to other disciplines and it can also be easily contextualized. However, these advantages can be minimized if meaningless and poor teaching techniques are used in the class. The target of this work is to create a teaching unit for Plane Geometry about length and area for 1st of E.S.O. students through cooperative and problem based learning. These will be com...

  17. Tratamiento del concepto de ser vivo y los reinos monera, protista y fungi en la atención a la diversidad de 1º ESO

    OpenAIRE

    Nepote Gorriz, Ainara

    2012-01-01

    Los contenidos que se tratan en este proyecto intentando diseñar unos contenidos y actividades conceptualmente transparentes para el alumnado, se dividen en 2 grandes apartados: primero se trata el concepto de ser vivo con 3 actividades diferentes, y luego los reinos monera, protista y fungi. Los objetivos generales que se establecen para este proyecto son los siguientes: 1. Acercar el concepto de ser vivo a alumnos y a alumnas de atención de la diversidad de 1º ESO ampliand...

  18. A composite control method based on the adaptive RBFNN feedback control and the ESO for two-axis inertially stabilized platforms.

    Science.gov (United States)

    Lei, Xusheng; Zou, Ying; Dong, Fei

    2015-11-01

    Due to the nonlinearity and time variation of a two-axis inertially stabilized platform (ISP) system, the conventional feedback control cannot be utilized directly. To realize the control performance with fast dynamic response and high stabilization precision, the dynamic model of the ISP system is expected to match the ideal model which satisfies the desired control performance. Therefore, a composite control method based on the adaptive radial basis function neural network (RBFNN) feedback control and the extended state observer (ESO), is proposed for ISP. The adaptive RBFNN is proposed to generate the feedback control parameters online. Based on the state error information in the working process, the adaptive RBFNN can be constructed and optimized directly. Therefore, no priori training data is needed for the construction of the RBFNN. Furthermore, a linear second-order ESO is constructed to compensate for the composite disturbance. The asymptotic stability of the proposed control method has been proven by the Lyapunov stability theory. The applicability of the proposed method is validated by a series of simulations and flight tests. Copyright © 2015 ISA. Published by Elsevier Ltd. All rights reserved.

  19. Discovery of a Kiloparsec Extended Hard X-Ray Continuum and Fe-Kα from the Compton Thick AGN ESO 428-G014

    Science.gov (United States)

    Fabbiano, G.; Elvis, M.; Paggi, A.; Karovska, M.; Maksym, W. P.; Raymond, J.; Risaliti, G.; Wang, Junfeng

    2017-06-01

    We report the discovery of kiloparsec-scale diffuse emission in both the hard continuum (3-6 keV) and in the Fe-Kα line in the Compton thick (CT) Seyfert galaxy ESO 428-G014. This extended hard component contains at least ˜24% of the observed 3-8 keV emission, and follows the direction of the extended optical line emission (ionization cone) and radio jet. The extended hard component has ˜0.5% of the intrinsic 2-10 keV luminosity within the bi-cones. A uniform scattering medium of density 1 {{cm}}-3 would produce this luminosity in a 1 kpc path length in the bi-cones. Alternatively, higher column density molecular clouds in the disk of ESO 428-G014 may be responsible for these components. The continuum may also be enhanced by the acceleration of charged particles in the radio jet. The steeper spectrum (Γ ˜ 1.7 ± 0.4) of the hard continuum outside of the central 1.″5 radius nuclear region suggests a contribution of scattered/fluorescent intrinsic Seyfert emission. Ultrafast nuclear outflows cannot explain the extended Fe-Kα emission. This discovery suggests that we may need to revise the picture at the base of our interpretation of CT AGN spectra.

  20. Production of NY-ESO-1 peptide/DRB1*08:03 tetramers and ex vivo detection of CD4 T-cell responses in vaccinated cancer patients.

    Science.gov (United States)

    Mizote, Yu; Uenaka, Akiko; Isobe, Midori; Wada, Hisashi; Kakimi, Kazuhiro; Saika, Takashi; Kita, Shoichi; Koide, Yukari; Oka, Mikio; Nakayama, Eiichi

    2014-02-12

    We established CD4 T-cell clones, Mz-1B7, and Ue-21, which recognized the NY-ESO-1 121-138 peptide from peripheral blood mononuclear cells (PBMCs) of an esophageal cancer patient, E-2, immunized with an NY-ESO-1 protein and determined the NY-ESO-1 minimal epitopes. Minimal peptides recognized by Mz-1B7 and Ue-21 were NY-ESO-1 125-134 and 124-134, respectively, both in restriction to DRB1*08:03. Using a longer peptide, 122-135, and five other related peptides, including either of the minimal epitopes recognized by the CD4 T-cell clones, we investigated the free peptide/DR recognition on autologous EBV-B cells as APC and peptide/DR tetramer binding. The results showed a discrepancy between them. The tetramers with several peptides recognized by either Mz-1B7 or the Ue-21 CD4 T-cell clone did not bind to the respective clone. On the other hand, unexpected binding of the tetramer with the peptide not recognized by CD4 T-cells was observed. The clone Mz-1B7 did not recognize the free peptide 122-135 on APC, but the peptide 122-135/DRB1*08:03 tetramer bound to the TCR on those cells. The failure of tetramer production and the unexpected tetramer binding could be due to a subtly modified structure of the peptide/DR tetramer from the structure of the free peptide/DR molecule. We also demonstrated that the NY-ESO-1 123-135/DRB1*08:03 tetramer detected ex vivo CD4 T-cell responses in PBMCs from patients after NY-ESO-1 vaccination in immunomonitoring. Copyright © 2013 Elsevier Ltd. All rights reserved.

  1. RFA-based 589-nm guide star lasers for ESO VLT: a paradigm shift in performance, operational simplicity, reliability, and maintenance

    Science.gov (United States)

    Friedenauer, Axel; Karpov, Vladimir; Wei, Daoping; Hager, Manfred; Ernstberger, Bernhard; Clements, Wallace R. L.; Kaenders, Wilhelm G.

    2012-07-01

    Large telescopes equipped with adaptive optics require 20-25W CW 589-nm sources with emission linewidths of ~5 MHz. These Guide Star (GS) lasers should also be highly reliable and simple to operate and maintain for many years at the top of a mountain facility. Under contract from ESO, industrial partners TOPTICA and MPBC are nearing completion of the development of GS lasers for the ESO VLT, with delivery of the first of four units scheduled for December 2012. We report on the design and performance of the fully-engineered Pre-Production Unit (PPU), including system reliability/availability analysis, the successfully-concluded qualification testing, long-term component and system level tests and long-term maintenance and support planning. The chosen approach is based on ESO's patented narrow-band Raman Fiber Amplifier (EFRA) technology. A master oscillator signal from a linearly-polarized TOPTICA 20-mW, 1178-nm CW diode laser, with stabilized emission frequency and controllable linewidth up to a few MHz, is amplified in an MPBC polarization-maintaining (PM) RFA pumped by a high-power 1120-nm PM fiber laser. With efficient stimulated Brillouin scattering suppression, an unprecedented 40W of narrow-band RFA output has been obtained. This is then mode-matched into a resonant-cavity doubler with a free-spectral-range matching the sodium D2a to D2b separation, allowing simultaneous generation of an additional frequency component (D2b line) to re-pump the sodium atom electronic population. With this technique, the return flux can be increased without having to resort to electro-optical modulators and without the risk of introducing optical wave front distortions. The demonstrated output powers with doubling efficiencies >80% at 589 nm easily exceed the 20W design goal and require less than 700 W of electrical power. In summary, the fiber-based guide star lasers provide excellent beam quality and are modular, turn-key, maintenance-free, reliable, efficient, and ruggedized

  2. First Data Release of the ESO-ARO Public Survey SAMPLING—SMT “All-sky” Mapping of Planck Interstellar Nebulae in the Galaxy

    Science.gov (United States)

    Wang, Ke; Zahorecz, Sarolta; Cunningham, Maria R.; Tóth, L. Viktor; Liu, Tie; Lu, Xing; Wang, Yuan; Cosentino, Giuliana; Sung, Ren-Shiang; Sokolov, Vlas; Wang, Shen; Wang, Yuwei; Zhang, Zhiyu; Li, Di; Kim, Kee-Tae; Tatematsu, Ken’ichi; Testi, Leonardo; Wu, Yuefang; Yang, Ji; SAMPLING Collaboration

    2018-01-01

    We make the first data release (DR1) of the ongoing ESO Public Survey SAMPLING (http://dx.doi.org/10.7910/DVN/0L8NHX). DR1 comprises of 124 fields distributed in $70^\\circ < l < 216^\\circ$, $-35^\\circ < b < 25^\\circ$. The 12CO and 13CO (2-1) cubes are gridded in $8"$ pixels, with an effective resolution of $36"$. The channel width is 0.33 km/s and the RMS noise is $T_{\\rm mb}<0.2$ K. Once completed, SAMPLING and complementary surveys will initiate the first major step forward to characterize molecular clouds and star formation on truly Galactic scales.

  3. La Segunda Guerra Mundial en la programación didáctica de Geografía e Historia para 4º de la ESO

    OpenAIRE

    Fuente Pérez, Rubén de la

    2016-01-01

    Este trabajo de fin de Master propone una Unidad Didáctica de 4º de la ESO para la materia de Geografía e Historia y desarrolla más ampliamente el diseño de una Unidad Didáctica centrada en La Segunda Guerra Mundial debido al enorme peso que tuvo el conflicto armado para el desarrollo de la segunda mitad del siglo XX y sobre todo el XXI. En el trabajo que aquí se presenta nos vamos a encontrar con una primera parte donde se expondrá el contexto actual de la enseñanza secundaria en Españ...

  4. Relación entre los hábitos familiares y el rendimiento académico en alumnos de 3º de ESO en Ciencias Naturales

    OpenAIRE

    González-Carrasco, Silvia

    2014-01-01

    El objeto de la presente investigación es el estudio de la relación existente entre los hábitos familiares y el rendimiento académico de los alumnos. Dicho estudio se realiza mediante el examen del marco teórico y su contraste empírico a través del análisis de una población de estudiantes de 3º de ESO del Instituto Olorda de Sant Feliu de Llobregat. Se trata de un centro público, situado en un barrio obrero de una ciudad del extrarradio de Barcelona. Para la obtención de los...

  5. NY-ESO-1 TCR single edited stem and central memory T cells to treat multiple myeloma without graft-versus-host disease.

    Science.gov (United States)

    Mastaglio, Sara; Genovese, Pietro; Magnani, Zulma; Ruggiero, Eliana; Landoni, Elisa; Camisa, Barbara; Schiroli, Giulia; Provasi, Elena; Lombardo, Angelo; Reik, Andreas; Cieri, Nicoletta; Rocchi, Martina; Oliveira, Giacomo; Escobar, Giulia; Casucci, Monica; Gentner, Bernhard; Spinelli, Antonello; Mondino, Anna; Bondanza, Attilio; Vago, Luca; Ponzoni, Maurilio; Ciceri, Fabio; Holmes, Michael C; Naldini, Luigi; Bonini, Chiara

    2017-08-03

    Transfer of T-cell receptors (TCRs) specific for tumor-associated antigens is a promising approach for cancer immunotherapy. We developed the TCR gene editing technology that is based on the knockout of the endogenous TCR α and β genes, followed by the introduction of tumor-specific TCR genes, and that proved safer and more effective than conventional TCR gene transfer. Although successful, complete editing requires extensive cell manipulation and 4 transduction procedures. Here we propose a novel and clinically feasible TCR "single editing" (SE) approach, based on the disruption of the endogenous TCR α chain only, followed by the transfer of genes encoding for a tumor-specific TCR. We validated SE with the clinical grade HLA-A2 restricted NY-ESO-1 157-165 -specific TCR. SE allowed the rapid production of high numbers of tumor-specific T cells, with optimal TCR expression and preferential stem memory and central memory phenotype. Similarly to unedited T cells redirected by TCR gene transfer (TCR transferred [TR]), SE T cells efficiently killed NY-ESO-1 pos targets; however, although TR cells proved highly alloreactive, SE cells showed a favorable safety profile. Accordingly, when infused in NSG mice previously engrafted with myeloma, SE cells mediated tumor rejection without inducing xenogeneic graft-versus-host disease, thus resulting in significantly higher survival than that observed in mice treated with TR cells. Overall, single TCR gene editing represents a clinically feasible approach that is able to increase the safety and efficacy of cancer adoptive immunotherapy. © 2017 by The American Society of Hematology.

  6. Major Histocompatibility Complex (MHC) Class I Processing of the NY-ESO-1 Antigen Is Regulated by Rpn10 and Rpn13 Proteins and Immunoproteasomes following Non-lysine Ubiquitination.

    Science.gov (United States)

    Golnik, Richard; Lehmann, Andrea; Kloetzel, Peter-Michael; Ebstein, Frédéric

    2016-04-15

    The supply of MHC class I-restricted peptides is primarily ensured by the degradation of intracellular proteins via the ubiquitin-proteasome system. Depending on the target and the enzymes involved, ubiquitination is a process that may dramatically vary in terms of linkages, length, and attachment sites. Here we identified the unique lysine residue at position 124 of the NY-ESO-1 cancer/testis antigen as the acceptor site for the formation of canonical Lys-48-linkages. Interestingly, a lysine-less form of NY-ESO-1 was as efficient as its wild-type counterpart in supplying the HLA-A*0201-restricted NY-ESO-1157-165 antigenic peptide. In fact, we show that the regulation of NY-ESO-1 processing by the ubiquitin receptors Rpn10 and Rpn13 as a well as by the standard and immunoproteasome is governed by non-canonical ubiquitination on non-lysine sites. In summary, our data underscore the significance of atypical ubiquitination in the modulation of MHC class I antigen processing. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  7. Major Histocompatibility Complex (MHC) Class I Processing of the NY-ESO-1 Antigen Is Regulated by Rpn10 and Rpn13 Proteins and Immunoproteasomes following Non-lysine Ubiquitination*

    Science.gov (United States)

    Golnik, Richard; Lehmann, Andrea; Kloetzel, Peter-Michael; Ebstein, Frédéric

    2016-01-01

    The supply of MHC class I-restricted peptides is primarily ensured by the degradation of intracellular proteins via the ubiquitin-proteasome system. Depending on the target and the enzymes involved, ubiquitination is a process that may dramatically vary in terms of linkages, length, and attachment sites. Here we identified the unique lysine residue at position 124 of the NY-ESO-1 cancer/testis antigen as the acceptor site for the formation of canonical Lys-48-linkages. Interestingly, a lysine-less form of NY-ESO-1 was as efficient as its wild-type counterpart in supplying the HLA-A*0201-restricted NY-ESO-1157–165 antigenic peptide. In fact, we show that the regulation of NY-ESO-1 processing by the ubiquitin receptors Rpn10 and Rpn13 as a well as by the standard and immunoproteasome is governed by non-canonical ubiquitination on non-lysine sites. In summary, our data underscore the significance of atypical ubiquitination in the modulation of MHC class I antigen processing. PMID:26903513

  8. First-in-Human Treatment With a Dendritic Cell-targeting Lentiviral Vector-expressing NY-ESO-1, LV305, Induces Deep, Durable Response in Refractory Metastatic Synovial Sarcoma Patient.

    Science.gov (United States)

    Pollack, Seth M; Lu, Hailing; Gnjatic, Sacha; Somaiah, Neeta; O'Malley, Ryan B; Jones, Robin L; Hsu, Frank J; Ter Meulen, Jan

    2017-10-01

    Effective induction of antitumor T cells is a pivotal goal of cancer immunotherapy. To this end, lentiviral vectors (LV) are uniquely poised to directly prime CD8 T-cell responses via transduction of dendritic cells in vivo and have shown promise as active cancer therapeutics in preclinical tumor models. However, until now, significant barriers related to production and regulation have prevented their widespread use in the clinic. We developed LV305, a dendritic cell-targeting, integration-deficient, replication incompetent LV from the ZVex platform, encoding the full-length cancer-testis antigen NY-ESO-1. LV305 is currently being evaluated in phase 1 and 2 trials in metastatic recurrent cancer patients with NY-ESO-1 positive solid tumors as a single agent and in combination with anti-PD-L1. Here we report on the first patient treated with LV305, a young woman with metastatic, recurrent, therapy-refractive NY-ESO-1 synovial sarcoma. The patient developed a robust NY-ESO-1-specific CD4 and CD8 T-cell response after 3 intradermal injections with LV305, and subsequently over 85% disease regression that is continuing for >2.5 years posttherapy. No adverse events >grade 2 occurred. This case demonstrates that LV305 can be safely administered and has the potential to induce a significant clinical benefit and immunologic response in a patient with advanced stage cancer.

  9. Circulating CD4+ T cells that produce IL4 or IL17 when stimulated by melan-A but not by NY-ESO-1 have negative impacts on survival of patients with stage IV melanoma.

    Science.gov (United States)

    Zelba, Henning; Weide, Benjamin; Martens, Alexander; Derhovanessian, Evelyna; Bailur, Jithendra Kini; Kyzirakos, Christina; Pflugfelder, Annette; Eigentler, Thomas K; Di Giacomo, Anna Maria; Maio, Michele; Aarntzen, Erik H J G; de Vries, Jolanda; Sucker, Antje; Schadendorf, Dirk; Büttner, Petra; Garbe, Claus; Pawelec, Graham

    2014-08-15

    We initially observed that the presence of circulating NY-ESO-1- and/or Melan-A-specific T cells in patients with stage IV melanoma was significantly associated with prolonged survival. Here, we report the ways in which the phenotypes and functions of these T cells differentially affect survival in patients preselected for NY-ESO-1 and/or Melan-A reactivity. We assayed functional antigen-reactive T cells recognizing NY-ESO-1 and/or Melan-A after in vitro stimulation using overlapping peptide pools. After restimulation, we assayed six cytokines simultaneously by intracellular cytokine staining. This allowed us to analyze the functional antigen response of both CD4(+) and CD8(+) T cells at the single-cell level. We observed that NY-ESO-1 stimulated mainly CD4(+) T cells, whereas Melan-A more often stimulated CD8(+) T cells. NY-ESO-1 reactivity was not associated with an additional impact on survival, whether CD4(+) T cells, CD8(+) T cells, or both types of T cells were responding. In contrast, recognition of Melan-A by CD4(+) T cells was associated with reduced survival in our cohort of patients preselected for NY-ESO-1 and/or Melan-A reactivity (that is, in patients with exceptionally long survival). We further observed a negative effect on survival in patients with CD4(+) T cells producing IL4 and IL17 upon Melan-A stimulation. Their prognosis was comparable to patients without any Melan-A reactivity. The nature and prognostic impact of specific T-cell responses is different according to targeted antigen. Independent from phenotype and functional aspects, NY-ESO-1 reactivity is associated with good prognosis. In terms of Melan-A, antigen-specific CD8(+) but not CD4(+) responses are associated with prolonged survival. Clin Cancer Res; 20(16); 4390-9. ©2014 AACR. ©2014 American Association for Cancer Research.

  10. Doing science in order to communicate about science from 1st course of ESO: learning to think, to read, to make, to communicate and to write science

    Directory of Open Access Journals (Sweden)

    María del Pilar Menoyo Díaz

    2017-01-01

    Full Text Available This  article  presents  the  project  \\Doing  science  in  order  to  communicate  about  science  from  1st  course of ESO : learning to think, to read, to make to communicate and to write science", awarded with “Ethics and Science for schools" second price in 2015 by the Foundation Victor Grífols i Lucas. The project's aim is twofold: the first one is that students gradually achieve during the whole ESO's itinerary a high-quality scientific alphabetization from all the areas and thus acquiring scientific and linguistic competencies that qualify  them  to  ask  themselves  questions  that  can  be  answered  through  by  research.  Its  second  goal  is  to encourage  students  to  ethically  process  sources  of  information  and  data  gathering  as  well  as  to  make  a sustainable use of the available resources. Communication and external dissemination of the process and results of the research is encouraged in two main ways. The first one, is achieved by using Juan Manuel Zafra  high-school's  website  as  teaching-learning  tool:  both  in  the  private  area  (Moodle  and  the  public area (with an on-line magazine, news, tweets and blogs. Moreover, students are encouraged to participate in  young  researchers  meetings,  as  Exporecerca  Jove,  Jóvenes  Investigadores,  Galiciencia  among  others research seminars. This article propounds a progression of the scientific and linguistic competences along the  four  courses  of  ESO  as  well  as  proposing  frames  in  which  the  student  body  can  be  initiated  in  the research process.

  11. Detection and Tracking of NY-ESO-1-Specific CD8+ T Cells by High-Throughput T Cell Receptor β (TCRB) Gene Rearrangements Sequencing in a Peptide-Vaccinated Patient.

    Science.gov (United States)

    Miyai, Manami; Eikawa, Shingo; Hosoi, Akihiro; Iino, Tamaki; Matsushita, Hirokazu; Isobe, Midori; Uenaka, Akiko; Udono, Heiichiro; Nakajima, Jun; Nakayama, Eiichi; Kakimi, Kazuhiro

    2015-01-01

    Comprehensive immunological evaluation is crucial for monitoring patients undergoing antigen-specific cancer immunotherapy. The identification and quantification of T cell responses is most important for the further development of such therapies. Using well-characterized clinical samples from a high responder patient (TK-f01) in an NY-ESO-1f peptide vaccine study, we performed high-throughput T cell receptor β-chain (TCRB) gene next generation sequencing (NGS) to monitor the frequency of NY-ESO-1-specific CD8+ T cells. We compared these results with those of conventional immunological assays, such as IFN-γ capture, tetramer binding and limiting dilution clonality assays. We sequenced human TCRB complementarity-determining region 3 (CDR3) rearrangements of two NY-ESO-1f-specific CD8+ T cell clones, 6-8L and 2F6, as well as PBMCs over the course of peptide vaccination. Clone 6-8L possessed the TCRB CDR3 gene TCRBV11-03*01 and BJ02-01*01 with amino acid sequence CASSLRGNEQFF, whereas 2F6 possessed TCRBV05-08*01 and BJ02-04*01 (CASSLVGTNIQYF). Using these two sequences as models, we evaluated the frequency of NY-ESO-1-specific CD8+ T cells in PBMCs ex vivo. The 6-8L CDR3 sequence was the second most frequent in PBMC and was present at high frequency (0.7133%) even prior to vaccination, and sustained over the course of vaccination. Despite a marked expansion of NY-ESO-1-specific CD8+ T cells detected from the first through 6th vaccination by tetramer staining and IFN-γ capture assays, as evaluated by CDR3 sequencing the frequency did not increase with increasing rounds of peptide vaccination. By clonal analysis using 12 day in vitro stimulation, the frequency of B*52:01-restricted NY-ESO-1f peptide-specific CD8+ T cells in PBMCs was estimated as only 0.0023%, far below the 0.7133% by NGS sequencing. Thus, assays requiring in vitro stimulation might be underestimating the frequency of clones with lower proliferation potential. High-throughput TCRB sequencing using NGS

  12. Deep Chandra Observations of ESO 428-G014. II. Spectral Properties and Morphology of the Large-scale Extended X-Ray Emission

    Science.gov (United States)

    Fabbiano, G.; Paggi, A.; Karovska, M.; Elvis, M.; Maksym, W. P.; Risaliti, G.; Wang, Junfeng

    2018-03-01

    We present a deep Chandra spectral and spatial study of the kpc-scale diffuse X-ray emission of the Compton-thick (CT) active galactic nucleus (AGN) ESO 428-G014. The entire spectrum is best fit with composite photoionization + thermal models. The diffuse emission is more extended at lower energies (cross-cone direction, where the AGN emission would be mostly obscured by the torus in the standard AGN model. Our results suggest that the transmission of the obscuring region in the cross-cone direction is ∼10% of that in the cone direction. In the 0.3–1.5 keV band, the ratio of cross-cone to cone photons increases to ∼84%, suggesting an additional soft diffuse emission component disjoint from the AGN. This could be due to hot ISM trapped in the potential of the galaxy. The luminosity of this component, ∼5 × 1038 erg s‑1, is roughly consistent with the thermal component suggested by the spectral fits in the 170–900 pc annulus.

  13. INTEGRAL, XMM-Newton and ESO/NTT identification of AX J1749.1-2733: an obscured and probably distant Be/X-ray binary

    Science.gov (United States)

    Zurita Heras, J. A.; Chaty, S.

    2008-10-01

    Context: AX J1749.1-2733 is an unclassified transient X-ray source discovered during surveys by ASCA in 1993-1999. The transient behaviour and the short and bright flares of the source have led to the idea that it is part of the recently revealed subclass of supergiant fast X-ray transients. Aims: A multi-wavelength study in NIR, optical, X-rays, and hard X-rays of AX J1749.1-2733 is undertaken in order to determine its nature. Methods: Public INTEGRAL data and our target of opportunity observation with XMM-Newton were used to study the high-energy source through timing and spectral analysis. Multi-wavelength observations in optical and NIR with the ESO/NTT telescope were also performed to search for the counterpart. Results: AX J1749.1-2733 is a new high-mass X-ray binary pulsar with an orbital period of 185.5±1.1 days (or 185.5/f with f=2,3 or 4) and a spin period of 66 s, parameters typical of a Be/X-ray binary. The outbursts last 12 d. A spin-down of dot{P}=0.08 ± 0.02 s yr -1 is also observed, very likely due to the propeller effect. The most accurate X-ray position is RA (2000) =17h49m06.8s and Dec =-27°32 arcmin32.5 arcsec (uncertainty 2 arcsec). The high-energy broad-band spectrum is well-fitted with an absorbed powerlaw and a high-energy cutoff with values NH=20.1-1.3+1.5×1022 cm-2, Γ=1.0-0.3+0.1, and Ecut=21-3+5 keV. The only optical/NIR candidate counterpart within the X-ray error circle has magnitudes of R=21.9±0.1, I=20.92±0.09, J=17.42±0.03, H=16.71±0.02, and Ks=15.75±0.07, which points towards a Be star located far away (>8.5 kpc) and highly absorbed (NH˜ 1.7×1022 cm-2). The average 22-50 keV luminosity is 0.4-0.9×1036 erg s-1 during the long outbursts and 3×1036 erg s-1 during the bright flare that occurred on MJD 52891 for an assumed distance of 8.5 kpc. Based on observations made with 1) INTEGRAL, an ESA project with instruments and science data centre funded by ESA member states (especially the PI countries: Denmark, France, Germany

  14. Twins born in different environments? Nuclei of two dSphs: isolated galaxy KKS3 and ESO269-66, a close neighbor of NGC5128

    Science.gov (United States)

    Sharina, Margarita; Karachentsev, Igor; Kniazev, Alexei

    2015-08-01

    The close vicinity of giant neighbors determines the environmental mechanisms that have been considered responsible for the evolution of dwarf spheroidal galaxies (dSphs). In the recent years, Karachentsev and collaborators have reported on the discovery of a few truly isolated dSphs in the Local volume. This study focuses on one of these unusual objects, KKs3 (MV=-12.3 mag). It contains a massive globular cluster (GC) (MV=-8.5 mag) near its optical center. We have performed the estimation of its radial velocity using a medium-resolution spectrum obtained with the RSS spectrograph at the Southern African Large Telescope (SALT). The signal-to-noise ratio in the spectrum was sufficient to estimate the age and metallicity for the GC using simple stellar population models, and the methods of full spectrum fitting and Lick index diagnostic diagrams. The results contribute to the knowledge about the origin of massive star clusters and their host dSphs.In the same way we have analyzed another luminous GC (MV=-10) in the center of ESO269-66 (MB=-15.4), a close dSph neighbor of the giant S0 Cen A. The cluster was observed with SALT in the same instrumental configuration. The structure and star formation histories of the two galaxies look rather similar. Both of them have experienced several star-forming events. The most recent ones occurred 1÷2 Gyr ago, and most powerful bursts happened 12÷14 Gyrs ago. Our analysis has shown that both GCs appear to be 1÷2 Gyr younger and 0.2÷0.3 dex more metal-rich than the most ancient metal-poor stars in the host dSphs. We examine signatures of multiple stellar population in the GCs using out data. Since central star-forming bursts were extended in time, the massive clusters might be considered as nuclei of the galaxies.

  15. The Gaia-ESO Survey: radial distribution of abundances in the Galactic disc from open clusters and young-field stars

    Science.gov (United States)

    Magrini, L.; Randich, S.; Kordopatis, G.; Prantzos, N.; Romano, D.; Chieffi, A.; Limongi, M.; François, P.; Pancino, E.; Friel, E.; Bragaglia, A.; Tautvaišienė, G.; Spina, L.; Overbeek, J.; Cantat-Gaudin, T.; Donati, P.; Vallenari, A.; Sordo, R.; Jiménez-Esteban, F. M.; Tang, B.; Drazdauskas, A.; Sousa, S.; Duffau, S.; Jofré, P.; Gilmore, G.; Feltzing, S.; Alfaro, E.; Bensby, T.; Flaccomio, E.; Koposov, S.; Lanzafame, A.; Smiljanic, R.; Bayo, A.; Carraro, G.; Casey, A. R.; Costado, M. T.; Damiani, F.; Franciosini, E.; Hourihane, A.; Lardo, C.; Lewis, J.; Monaco, L.; Morbidelli, L.; Sacco, G.; Sbordone, L.; Worley, C. C.; Zaggia, S.

    2017-06-01

    Context. The spatial distribution of elemental abundances in the disc of our Galaxy gives insights both on its assembly process and subsequent evolution, and on the stellar nucleogenesis of the different elements. Gradients can be traced using several types of objects as, for instance, (young and old) stars, open clusters, HII regions, planetary nebulae. Aims: We aim to trace the radial distributions of abundances of elements produced through different nucleosynthetic channels - the α-elements O, Mg, Si, Ca and Ti, and the iron-peak elements Fe, Cr, Ni and Sc - by use of the Gaia-ESO IDR4 results for open clusters and young-field stars. Methods: From the UVES spectra of member stars, we have determined the average composition of clusters with ages > 0.1 Gyr. We derived statistical ages and distances of field stars. We traced the abundance gradients using the cluster and field populations and compared them with a chemo-dynamical Galactic evolutionary model. Results: The adopted chemo-dynamical model, with the new generation of metallicity-dependent stellar yields for massive stars, is able to reproduce the observed spatial distributions of abundance ratios, in particular the abundance ratios of [O/Fe] and [Mg/Fe] in the inner disc (5 kpc Tables A.1-A.4 are also available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/603/A2

  16. Performance of the ESO PDS

    Science.gov (United States)

    Grosboel, P. J.

    1984-01-01

    The astrometric and photometric performance of the PDS 1010A microdensitometer are discussed, including the tests used for checking it. The instrument was shown to satisfy operational requirements for photometric measurements of astronomical plates with respect to dynamic range and stability.

  17. Supernova 1998bw In ESO 184-G82. Repr. from Central Bureau for Astronomical Telegrams, no. 6969,10 Jul. 1998

    Science.gov (United States)

    Kay, L. E.; Halpern, Jules P.; Leighly, K. M.; Heathcote, S.; Magalhaes, A. M.; Oliversen, Ronald (Technical Monitor)

    2001-01-01

    Over the wavelength range 390-750 nm, we measure intrinsic linear polarization of 0.53 +/- 0.08 percent at position angle 49 +/- 3 deg, after correcting for Galactic interstellar polarization using the star HD 184100, which has polarization of 0.75 +/- 0.01 percent at p.a. 176.5 +/- 2.5 deg. This measured interstellar polarization is consistent with the Galactic extinction in this direction, estimated to be E(B-V) = 0.059 from IRAS maps, or E(B-V) = 0.079 from 21-cm H I. Interstellar polarization in the host galaxy ESO 184-G82 is expected to be negligible based on the relative absence of Na I D absorption at z = 0.00841 +/- 0.00005, the redshift of the environment of the supernova from narrow H II region emission lines in its spectrum. Polarization appears highest in between emission features in the total flux spectrum, which strengthens the interpretation of the polarization as intrinsic to the supernova. This modest polarization is less than that of some type-II supernovae, but greater than that of type-Ia supernovae, which are generally unpolarized. This supports the interpretation of SN 1998bw, a peculiar type-Ic supernova, as a core-collapse event in which the observed polarization is due to moderate asymmetry in either the photosphere of the ejecta or an overlying scattering envelope. However, this result does not strongly constrain arguments about whether some supernovae emit gamma-ray bursts, since such emission may come from a mildly relativistic shock associated with the radio emission and above the optical photosphere, without any requirements on beaming or orientation." A. V. Filippenko, University of California at Berkeley, comments on the total flux spectrum obtained above: "The spectrum most closely resembles those of the peculiar SN 1997ef, but perhaps evolving more slowly. It is not typical of type-Ic supernovae; indeed, the spectrum does not match any of the known spectral classes, but perhaps 'peculiar type Ic' is the best choice at this time

  18. A phase I vaccination study with dendritic cells loaded with NY-ESO-1 and α-galactosylceramide: induction of polyfunctional T cells in high-risk melanoma patients.

    Science.gov (United States)

    Gasser, Olivier; Sharples, Katrina J; Barrow, Catherine; Williams, Geoffrey M; Bauer, Evelyn; Wood, Catherine E; Mester, Brigitta; Dzhelali, Marina; Caygill, Graham; Jones, Jeremy; Hayman, Colin M; Hinder, Victoria A; Macapagal, Jerome; McCusker, Monica; Weinkove, Robert; Painter, Gavin F; Brimble, Margaret A; Findlay, Michael P; Dunbar, P Rod; Hermans, Ian F

    2018-02-01

    Vaccines that elicit targeted tumor antigen-specific T-cell responses have the potential to be used as adjuvant therapy in patients with high risk of relapse. However, the responses induced by vaccines in cancer patients have generally been disappointing. To improve vaccine function, we investigated the possibility of exploiting the immunostimulatory capacity of type 1 Natural killer T (NKT) cells, a cell type enriched in lymphoid tissues that can trigger improved antigen-presenting function in dendritic cells (DCs). In this phase I dose escalation study, we treated eight patients with high-risk surgically resected stage II-IV melanoma with intravenous autologous monocyte-derived DCs loaded with the NKT cell agonist α-GalCer and peptides derived from the cancer testis antigen NY-ESO-1. Two synthetic long peptides spanning defined immunogenic regions of the NY-ESO-1 sequence were used. This therapy proved to be safe and immunologically effective, inducing increases in circulating NY-ESO-1-specific T cells that could be detected directly ex vivo in seven out of eight patients. These responses were achieved using as few as 5 × 10 5 peptide-loaded cells per dose. Analysis after in vitro restimulation showed increases in polyfunctional CD4 + and CD8 + T cells that were capable of manufacturing two or more cytokines simultaneously. Evidence of NKT cell proliferation and/or NKT cell-associated cytokine secretion was seen in most patients. In light of these strong responses, the concept of including NKT cell agonists in vaccine design requires further investigation.

  19. Influencia del uso de las TIC en alumnos y profesores de E.S.O. y su aplicación en la asignatura de Matemáticas

    OpenAIRE

    Miera-Espiga, María

    2014-01-01

    En ocasiones, los alumnos suelen experimentar grandes dificultades en el área de matemáticas y esto les conduce a caer en una desmotivación. Con este trabajo se pretende desarrollar una propuesta de integración de las TIC que contribuya a la mejora del rendimiento académico de los alumnos de secundaria en el área de matemáticas. Para ello se ha realizado una revisión bibliográfica sobre el tema y se ha llevado a cabo un estudio de campo con 180 alumnos de ESO y 12 profesores de matemáticas, t...

  20. Enseñanza y aprendizaje de la física a través de la lectura del quijote en 4.º de eso. Las leyes de Newton y la aventura de los molinos de viento

    OpenAIRE

    Franco Mariscal, Antonio

    2013-01-01

    En este trabajo se presenta el efecto que produce en el aprendizaje de temas de dinámica, respecto a una metodología tradicional de nivel de 4.º de ESO (16 años), el uso de una metodología innovadora basada en la lectura de la famosa aventura de los molinos de viento del Quijote, donde el alumno juega un papel activo, plantea y resuelve sus propios problemas científicos y se convierte en el verdadero protagonista de su aprendizaje. Los resultados obtenidos sugieren que la metodología basada e...

  1. Estilos de aprendizaje en geometría: Enseñanza de la geometría en 2º de la ESO mediante un blog según el modelo VARK

    OpenAIRE

    Blasco-Esteve, María Teresa

    2013-01-01

    El presente trabajo propone la utilización de un blog para enseñar el bloque de geometría de matemáticas de 2º de la ESO, teniendo en cuenta los estilos de aprendizaje según el Modelo VARK1.En primer lugar se profundiza en las características del modelo VARK sobre el que se basa este proyecto. Seguidamente, se analiza las características principales de los blogs y las posibilidades educativas que éstos ofrecen. También se realiza un análisis del material habitualmente utilizado para ...

  2. The Gaia-ESO Survey and CSI 2264: Substructures, disks, and sequential star formation in the young open cluster NGC 2264

    Science.gov (United States)

    Venuti, L.; Prisinzano, L.; Sacco, G. G.; Flaccomio, E.; Bonito, R.; Damiani, F.; Micela, G.; Guarcello, M. G.; Randich, S.; Stauffer, J. R.; Cody, A. M.; Jeffries, R. D.; Alencar, S. H. P.; Alfaro, E. J.; Lanzafame, A. C.; Pancino, E.; Bayo, A.; Carraro, G.; Costado, M. T.; Frasca, A.; Jofré, P.; Morbidelli, L.; Sousa, S. G.; Zaggia, S.

    2018-01-01

    Context. Reconstructing the structure and history of young clusters is pivotal to understanding the mechanisms and timescales of early stellar evolution and planet formation. Recent studies suggest that star clusters often exhibit a hierarchical structure, possibly resulting from several star formation episodes occurring sequentially rather than a monolithic cloud collapse. Aims: We aim to explore the structure of the open cluster and star-forming region NGC 2264 ( 3 Myr), which is one of the youngest, richest and most accessible star clusters in the local spiral arm of our Galaxy; we link the spatial distribution of cluster members to other stellar properties such as age and evolutionary stage to probe the star formation history within the region. Methods: We combined spectroscopic data obtained as part of the Gaia-ESO Survey (GES) with multi-wavelength photometric data from the Coordinated Synoptic Investigation of NGC 2264 (CSI 2264) campaign. We examined a sample of 655 cluster members, with masses between 0.2 and 1.8 M⊙ and including both disk-bearing and disk-free young stars. We used Teff estimates from GES and g,r,i photometry from CSI 2264 to derive individual extinction and stellar parameters. Results: We find a significant age spread of 4-5 Myr among cluster members. Disk-bearing objects are statistically associated with younger isochronal ages than disk-free sources. The cluster has a hierarchical structure, with two main blocks along its latitudinal extension. The northern half develops around the O-type binary star S Mon; the southern half, close to the tip of the Cone Nebula, contains the most embedded regions of NGC 2264, populated mainly by objects with disks and ongoing accretion. The median ages of objects at different locations within the cluster, and the spatial distribution of disked and non-disked sources, suggest that star formation began in the north of the cluster, over 5 Myr ago, and was ignited in its southern region a few Myr later

  3. La consultoría en el país. ¿Deben o no participar las universidades en esos procesos?

    Directory of Open Access Journals (Sweden)

    Gonzalo Torres Cadena

    2008-05-01

    Full Text Available La participación de las universidades en trabajos de consultoría es aún materia de controversia entre los consejos profesionales, las agencias de consultoría, los profesionales independientes, los profesores, los egresados y las mismas universidades. ¿Debe una universidad orientar sus esfuerzos exclusivamente a la docencia y a la investigación? Si una universidad decidiera, por su propia iniciativa, incursionar en el campo de la consultoría, ¿es aceptable su participación en convocatorias públicas, al tiempo que lo hacen otros proponentes?, ¿lo es plantearle abiertamente una competencia a sus propios egresados, cuando ellos se desempeñan como consultores?, ¿debe comprometerse con trabajos de consultoría que buscan un resultado concreto y medible, o debe limitarse a los trabajos de acompañamiento a las empresas contratantes en busca de una solución?, ¿cualquier oferta de consultoría es aceptable, si el talento para desarrollarla reside en la universidad?, ¿debe ser la consultoría un ejercicio eminentemente individual, que desarrolla el profesor en el tiempo que la universidad le concede para esos fines, o debe ser un trabajo colegiado en el cual la universidad asume plena responsabilidad sobre el resultado? Después de recorrer la experiencia de algunas universidades de reconocido prestigio alrededor de estos interrogantes, se expresan las condiciones bajo las cuales los trabajos de consultoría en las universidades se constituyen en un medio necesario y eficaz para el logro de su misión institucional./ University involvement in consulting practices is still a controversial matter among professional councils, consultancy firms, independent practitioners, faculty members and, even, university boards. Should a university concentrate exclusively in the teaching and research? If a university decides to offer consulting services to different prospects, Should it participate in public bids, then competing with other contestants

  4. Estudio sobre la evolución de los modelos mentales de estudiantes de 4º de ESO cuando observan, reflexionan y discuten sobre la combustión

    Directory of Open Access Journals (Sweden)

    Vanessa Sesto

    2017-01-01

    Full Text Available El propósito de este estudio fue analizar la evolución de los modelos mentales de estudiantes de secundaria sobre fenómenos que implican cambios en la materia al tener la oportunidad de observar, reflexionar y discutir sobre ellos. En trabajos anteriores ya se han identificado los modelos de los estudiantes sobre esta temática; sin embargo, aún se tiene poco conocimiento de cómo esos modelos evolucionan con la interacción social. Para alcanzar este objetivo se propuso como actividad la combustión de una vela dentro de un recipiente de cristal. Los participantes eran ocho estudiantes de 4º de ESO. La recogida de datos se realizó por medio de cuestionarios y grabaciones en audio y vídeo al realizar la actividad en pequeño grupo. Los resultados muestran una cierta evolución en los modelos. Al principio, los estudiantes utilizan los modelos de desaparición y transmutación. Después de la discusión, algunos estudiantes ya utilizan el modelo de reacción química.

  5. Vaccination of stage III/IV melanoma patients with long NY-ESO-1 peptide and CpG-B elicits robust CD8+and CD4+T-cell responses with multiple specificities including a novel DR7-restricted epitope.

    Science.gov (United States)

    Baumgaertner, P; Costa Nunes, C; Cachot, A; Maby-El Hajjami, H; Cagnon, L; Braun, M; Derré, L; Rivals, J-P; Rimoldi, D; Gnjatic, S; Abed Maillard, S; Marcos Mondéjar, P; Protti, M P; Romano, E; Michielin, O; Romero, P; Speiser, D E; Jandus, C

    2016-01-01

    Long synthetic peptides and CpG-containing oligodeoxynucleotides are promising components for cancer vaccines. In this phase I trial, 19 patients received a mean of 8 (range 1-12) monthly vaccines s.c. composed of the long synthetic NY-ESO-1 79-108 peptide and CpG-B (PF-3512676), emulsified in Montanide ISA-51. In 18/18 evaluable patients, vaccination induced antigen-specific CD8 + and CD4 + T-cell and antibody responses, starting early after initiation of immunotherapy and lasting at least one year. The T-cells responded antigen-specifically, with strong secretion of IFNγ and TNFα, irrespective of patients' HLAs. The most immunogenic regions of the vaccine peptide were NY-ESO-1 89-102 for CD8 + and NY-ESO-1 83-99 for CD4 + T-cells. We discovered a novel and highly immunogenic epitope (HLA-DR7/NY-ESO-1 87-99 ); 7/7 HLA-DR7 + patients generated strong CD4 + T-cell responses, as detected directly ex vivo with fluorescent multimers. Thus, vaccination with the long synthetic NY-ESO-1 79-108 peptide combined with the strong immune adjuvant CpG-B induced integrated, robust and functional CD8 + and CD4 + T-cell responses in melanoma patients, supporting the further development of this immunotherapeutic approach.

  6. Gaia-ESO Survey: Empirical classification of VLT/Giraffe stellar spectra in the wavelength range 6440-6810 Å in the γ Velorum cluster, and calibration of spectral indices

    Science.gov (United States)

    Damiani, F.; Prisinzano, L.; Micela, G.; Randich, S.; Gilmore, G.; Drew, J. E.; Jeffries, R. D.; Frémat, Y.; Alfaro, E. J.; Bensby, T.; Bragaglia, A.; Flaccomio, E.; Lanzafame, A. C.; Pancino, E.; Recio-Blanco, A.; Sacco, G. G.; Smiljanic, R.; Jackson, R. J.; de Laverny, P.; Morbidelli, L.; Worley, C. C.; Hourihane, A.; Costado, M. T.; Jofré, P.; Lind, K.; Maiorca, E.

    2014-06-01

    We present a study of spectral diagnostics available from optical spectra with R = 17 000 obtained with the VLT/Giraffe HR15n setup, using observations from the Gaia-ESO Survey, on the γ Vel young cluster, with the purpose of classifying these stars and finding their fundamental parameters. We define several spectroscopic indices, sampling the amplitude of TiO bands, the Hα line core and wings, and temperature- and gravity-sensitive sets of lines, each useful as a Teff or log g indicator over a limited range of stellar spectral types. Hα line indices are also useful as chromospheric activity or accretion indicators. Furthermore, we use all indices to define additional global Teff- and log g-sensitive indices τ and γ, valid for the entire range of types in the observed sample. We find a clear difference between gravity indices of main-sequence and pre-main-sequence stars, as well as a much larger difference between these and giant stars. The potentially great usefulness of the (γ,τ) diagram as a distance-independent age measurement tool for young clusters is discussed. We discuss the effect on the defined indices of classical T Tauri star veiling, which is however detected in only a few stars in the present sample. Then, we present tests and calibrations of these indices, on the basis of both photometry and literature reference spectra, from the UVES Paranal Observatory Projectand the ELODIE 3.1 Library. The known properties of these stars, spanning a wide range of stellar parameters, enable us to obtain a good understanding of the performances of our new spectral indices. For non-peculiar stars with known temperature, gravity, and metallicity, we are able to calibrate quantitatively our indices, and derive stellar parameters for a wide range of stellar types. To this aim, a new composite index is defined, providing a good metallicity indicator. The ability of our indices to select peculiar, or otherwise rare classes of stars is also established. For pre

  7. Evaluación de la implementación de las TIC en el centro educativo Pàlcam y propuesta de 2 TIC específicas para la asignatura de Biología en tercer curso de Educación Secundaria Obligatoria (ESO)

    OpenAIRE

    Pijoan-Marcos, Mónica

    2015-01-01

    Este trabajo trata de conocer el grado de integración y de implementación de las Tecnología de la Información y Comunicación (TIC) en las aulas de ESO en el colegio Pàlcam de Barcelona, así como de proponer 2 herramientas específicas para ser implementadas en la asignatura de Biología de 3º ESO del centro. Es importante tener en cuenta que Pàlcam tiene los medios para introducir las TIC en las aulas pero sin embargo los docentes actualmente desconocen la forma práctica de implementarlas en...

  8. Enfoque CTS en la enseñanza de la energía nuclear : análisis de su tratamiento en textos de física y química de la ESO

    OpenAIRE

    García-Carmona, Antonio

    2008-01-01

    En este artículo se muestran los resultados y las conclusiones de un estudio exploratorio sobre el enfoque ciencia-tecnología-sociedad (CTS), introducido en la enseñanza de la energía nuclear en Secundaria. Para ello, se analizan once libros de texto de las editoriales más utilizadas en la enseñanza de la física y química de 3º de Educación Secundaria Obligatoria (ESO), en la Comunidad Autónoma de Andalucía (España). In this paper, we present the results and conclusions of an exploratory s...

  9. ¿Qué estamos enseñando con los libros de texto? La electricidad y la electrónica de tecnología en 3º ESO

    Directory of Open Access Journals (Sweden)

    Carlos de Pro Chereguini

    2011-01-01

    Full Text Available Este trabajo trata de dar respuestas al interrogante: ¿Qué estamos enseñando con los libros de texto en Educación Secundaria Obligatoria? En primer lugar, revisamos los trabajos realizados sobre estos materiales en España y, a pesar de la importancia que tienen en nuestro sistema educativo, no hemos encontrado muchos que se hayan ocupado de aquellos que se elaboraron durante la Reforma LOE. Nos centramos en algunos elementos que consideramos fundamentales en estos recursos didácticos: la estructura de las lecciones y los tipos de contenidos (conceptos, procedimientos y actitudes a través del análisis de las actividades de aprendizaje planteadas por seis libros de texto de editoriales conocidas, relativas al bloque "Electricidad y electrónica" de Tecnología de 3º ESO, y su adecuación o no a la normativa vigente en la CARM. Los resultados muestran algunas de las carencias y deficiencias existentes, y que los libros de texto, en general, no se acomodan a lo que marca el currículum oficial.

  10. La división digital en el proceso de integración de las NTIC en la educación. Diferencias de género entre alumnos de E.S.O. de la comunidad valenciana

    Directory of Open Access Journals (Sweden)

    Bernardo GARGALLO LÓPEZ

    2016-04-01

    Full Text Available En este trabajo se analizan diferencias de género existentes en una muestra representativa de estudiantes de ESO de la Comunidad Valenciana en lo relativo a la integración de las TIC en el sistema educativo, desde una perspectiva univariada y multivariada. Estas diferencias se estudian en cuanto a disponibilidad y uso de Internet, formación, actitudes y valoración de sus repercusiones en la calidad de la educación. Los datos del estudio reflejan una cierta "brecha digital" favorable a los varones en lo concerniente a disponibilidad, uso y formación. Sin embargo, las actitudes de las chicas son aún más favorables a la integración de las TIC que las de los chicos, lo que induce a pensar que la división digital existente puede cerrarse con facilidad si las políticas y acciones que se desarrollen se orientan a compensar las diferencias existentes.

  11. Desempeño en el uso de pruebas en estudiantes 3º de ESO durante la resolución de un problema sobre alimentación humana

    Directory of Open Access Journals (Sweden)

    Beatriz Bravo Torija

    2014-01-01

    Full Text Available Este trabajo analiza el desempeño en el uso de pruebas de seis alumnos de 3º ESO de un grupo de diversificación curricular durante la resolución de un problema sobre alimentación humana basado en dos casos de adolescentes con trastornos alimenticios. Para proponer una solución los alumnos deben de utilizar tanto las pruebas como la teoría abordadas a lo largo de una unidad didáctica sobre alimentación y nutrición. Los resultados muestran que todos los estudiantes son capaces de extraer e interpretar información relevante, llegando la mitad de los estudiantes a integrarla en sus justificaciones. Además, dos de ellos relacionan datos de distintas fuentes con la teoría para justificar su conclusión. Favorecer el desempeño en el uso de pruebas ayuda por una parte a aplicar el conocimiento a problemas concretos y por otra promueve que los alumnos reconozcan la relación entre distintas actividades, siendo capaces de recuperar los datos proporcionados para construir una justificación.

  12. ¿Qué estamos enseñando con los libros de texto? La electricidad y la electrónica de Tecnología en 3º ESO

    Directory of Open Access Journals (Sweden)

    Carlos de Pro Chereguini

    Full Text Available Este trabajo trata de dar respuestas al interrogante: ¿Qué estamos enseñando con los libros de texto en Educación Secundaria Obligatoria? En primer lugar, revisamos los trabajos realizados sobre estos materiales en España y, a pesar de la importancia que tienen en nuestro sistema educativo, no hemos encontrado muchos quese hayan ocupado de aquellos que se elaboraron durante la Reforma LOE. Nos centramos en algunos elementos que consideramos fundamentales en estos recursos didácticos: la estructura de las lecciones y los tipos de contenidos (conceptos, procedimientos y actitudes a través del análisis de las actividades de aprendizajeplanteadas por seis libros de texto de editoriales conocidas, relativas al bloque “Electricidad y electrónica” de Tecnología de 3º ESO, y su adecuación o no a la normativa vigente en la CARM. Los resultados muestran algunas de las carencias y deficiencias existentes, y que los libros de texto, en general, no se acomodan a lo que marca el currículum oficial.

  13. Los monumentos funerarios hispanorromanos y su epigrafía en el Museo Nacional de Arte Romano de Mérida. Una propuesta didáctica para alumnos de Cultura Clásica y Latín de ESO y del Bachillerato / The funerary monuments in the Roman Hispania and their epigraphy in the National Museum of Roman Art of Merida. A didactic proposal addressed to students of Classical Culture and Latin in ESO and Bachillerato

    Directory of Open Access Journals (Sweden)

    Abel Morcillo León

    2010-01-01

    Full Text Available Resumen: El mundo funerario en la Hispania romana presenta una gran variedad en sus tipos monumentales. Del estudio de los monumentos y la tipología funeraria se extrae información no sólo cultural y religiosa, sino también referente a aspectos sociológicos, demográficos, epigráficos, artísticos e iconográficos. La antigua capital de la Lusitania romana, Augusta Emerita, albergaba en su solar una nutrida, variada e interesante muestra del mejor arte funerario hispanorromano. Presentamos, por ello, un recorrido por una selección de piezas del Museo Nacional de Arte Romano de Mérida, acompañado de una propuesta de actividades didácticas, destinado a alumnos de Cultura Clásica y Latín de E.S.O. y del Bachillerato.Summary: The funerary world in the Roman Hispania showed a great deal of monumental models. Through the study of its monuments and their typology, not only can we gather religious and cultural information but we can also draw conclusions regarding sociological, demographic, epigraphic, artistic and iconographic features. Emerita Augusta, the old capital of the Roman Lusitania, housed a wide range of the best Hispano-Roman funerary artistic manifestations. That is why we are presenting a tour around several pieces from the Roman Art Museum in Mérida, together with a series of didactic activities addressed to students of Classical Culture and Latin in E. S. O. (Secondary Compulsory Education and Bachillerato (Non Compulsory Secondary Education.

  14. Profesores de ESO y necesidades educativas especiales

    Directory of Open Access Journals (Sweden)

    Rosa María RODRÍGUEZ TEJADA

    2009-05-01

    Full Text Available RESUMEN: La diversidad del alumnado, algunos con fuertes dificultades de aprendizaje, es una realidad en nuestros institutos. Una realidad para la que los profesores de Secundaria no han sido preparados. Por otra parte, las actitudes del profesor hacia sus alumnos constituyen una premisa básica en Pedagogía. Basándose en ella, se han investigado las actitudes del profesorado de Educación Secundaria Obligatoria hacia las necesidades educativas especiales de sus alumnos. A fin de tener una visión más amplia y completa del problema en estudio y siguiendo las últimas tendencias en investigación educativa, se ha empleado metodología perteneciente al paradigma cuantitativo y cualitativo. En este artículo se refleja únicamente la parte de la investigación en la que se han usado técnicas cuantitativas. Las variables contempladas hacen referencia a las diferentes características personales y contextuales de los profesores de Educación Secundaria Obligatoria. La hipótesis de partida se ha visto confirmada. Las conclusiones de la investigación sirven para arrojar luz sobre este tema, trazando líneas a seguir para el mejor aprovechamiento de los recursos humanos en aras de la atención a la diversidad de los alumnos y, dentro de ella, la atención de los que tienen fuertes dificultades para aprender.ABSTRACT: The diversity of students, some of them with big learning dificulties, is a reality in our Secondary Schools. However, Secondary School’s teachers have not been trained for it. Taking into account that the teacher’s attitude is a basic premise in Pedagogy, we have worked on the Secondary School teacher’s attitude towards the students with special needs. In order to have a deeper and wider view of this question and following the latest tendencies in educative research techniques, we have used a methodology that belong to the quantitative and qualitative paradigm. This article refers only to the part of research that used the quantitative techniques. The variables used are related to the different personal and contextual characteristics of the teachers in Secondary Education. The hypothesis has been confirmed. The conclusions of the research throw some light on this topic, suggesting ways in which we can benefit from human resources towards the attention to diversity of students and specially to those who have big learning difficulties.

  15. Dear CERN and ESO Colleagues and Retirees

    CERN Multimedia

    Pension Fund

    2010-01-01

    Following approval by the CERN Pension Fund Governing Board, the CERN Pension Fund team is pleased to announce the new website address of your Pension Fund: http://pensionfund.cern.ch I take this opportunity to thank all the technical teams at CERN for the quality and rapidity of their support. Kind regards, Théodore Economou CEO, CERN Pension Fund

  16. ESO2 Optimization of Supermarket Refrigeration Systems

    DEFF Research Database (Denmark)

    Petersen, Lars Norbert; Madsen, Henrik; Heerup, Christian

    Supermarket refrigeration systems consists of a number of display cases, cooling cabinets and cold rooms connected to a central compressor pack. This configuration saves energy compared to placing a compressor at each cooling site. The classical control setup of a supermarket refrigeration system...... in the supermarket. The first approach to solve this problem is to design an overall control system which coordinates the compressor capacity and the current refrigeration load. The drawback of this approach is the complexity of the single controller. The solution is investigated in the first part of the report....... A second solution is investigated where only the compressor control is considered. This controller try to feed-forward the measured disturbances, i.e. opening and closing of the cooling site AKV’s. Last a performance analysis of the refrigeration system is performed....

  17. ¿Podemos desarrollar el pensamiento creativo a través de las Matemáticas? Investigación so-bre los procesos de resolución de problemas en didáctica de Matemáticas en dos centros educa-tivos de 4ºESO de A Coruña.

    OpenAIRE

    González, Jonás Ignacio

    2012-01-01

    La presente investigación es un intento de aproximación a la situación de la creatividad y las técnicas de pensamiento creativo en dos realidades educativas concretas. El objetivo general es investigar qué metodologías de enseñanza-aprendizaje de pensamiento y resolución creativa de problemas en Matemáticas utilizan los alumnos y profesores de Matemáticas de 4ºESO, en qué medida incluyen la creatividad como factor, y las posibilidades de aplicación de la creatividad en el aula. La investig...

  18. Relación entre los estilos de aprendizaje, el rendimiento en matemáticas y la elección de asignaturas optativas en alumnos de E.S.O. [Analysis of the relation between the learning styles, the performance in maths and the election of optional subjects in Secondary School

    Directory of Open Access Journals (Sweden)

    Ricardo Luengo González

    2005-10-01

    Full Text Available This article summarizes a quasi-experimental investigation conducted in Secondary School with two distinct objectives: establishing the possible relationship between a student’s learning style (from Honey-Alonso’s perspective and performance in math; and critically analyzing whether the selection process of optional courses in Compulsory Secondary School is based on objective, fair, and appropriate information about the student. The studied sample showed that there are significant relationships between the middle-high math performance level and an emphasis on reflexive and theoretical areas of study. It has also been found that the students in each optional course conform to a homogeneous subgroup with respect to the students’ performance and learning styles. El presente artículo resume una investigación cuasi-experimental realizada en Educación Secundaria, con dos objetivos bien diferenciados: establecer la posible relación entre las predominancias de los estilos de aprendizaje del alumno (desde la perspectiva de Honey-Alonso y el rendimiento en Matemáticas; analizar, de manera crítica, si el proceso orientador en la elección del espacio de optatividad en la E.S.O. se basa en una información objetiva y apropiada para el alumno. Se concluye que, en la muestra estudiada, existen relaciones significativas entre el rendimiento medio-alto en Matemáticas con una mayor predominancia en las áreas estilísticas teórica y reflexiva. Se confirma también que el alumnado de cada asignatura optativa conforma un subgrupo homogéneo en cuanto al rendimiento y los estilos de aprendizaje.

  19. La reflexión metalingüística de los alumnos de 4º de ESO, 2º de Bachillerato y 3º de Grado en Estudios Hispánicos que implica la alteración del orden oracional / The metalinguistic reflection of students in 4th grade Secondary School, 2nd of Bachillerato and 3rd year Degree in Hispanic Studies that involve the change of the sentence order

    OpenAIRE

    Raquel Badía Iborra

    2014-01-01

    Resumen: Este artículo presenta los datos de una investigación en la que se pretende estudiar cómo los alumnos de 4º de ESO, 2º de Bachillerato y 3º de Grado en Estudios Hispánicos realizan reflexiones metalingüísticas al verse obligados a explicar las diferencias de significado pragmático que implica la alteración del orden oracional (SVO) en español. Todos estos datos son extraídos a través de una prueba escrita realizada a dos parejas de alumnos de cada curso, un cuestionario en el que deb...

  20. Eclipse: ESO C Library for an Image Processing Software Environment

    Science.gov (United States)

    Devillard, Nicolas

    2011-12-01

    Written in ANSI C, eclipse is a library offering numerous services related to astronomical image processing: FITS data access, various image and cube loading methods, binary image handling and filtering (including convolution and morphological filters), 2-D cross-correlation, connected components, cube and image arithmetic, dead pixel detection and correction, object detection, data extraction, flat-fielding with robust fit, image generation, statistics, photometry, image-space resampling, image combination, and cube stacking. It also contains support for mathematical tools like random number generation, FFT, curve fitting, matrices, fast median computation, and point-pattern matching. The main feature of this library is its ability to handle large amounts of input data (up to 2GB in the current version) regardless of the amount of memory and swap available on the local machine. Another feature is the very high speed allowed by optimized C, making it an ideal base tool for programming efficient number-crunching applications, e.g., on parallel (Beowulf) systems.

  1. eso-na-nceij-ui-y-19-9-7

    Indian Academy of Sciences (India)

    Page 1. Science Smiles. RKLaxman. It saves a lot of bother, If you programme it, it goes about on its own taking pictures of tourist and historical interests, and returns. R -E-S-O-NA-N-C-E-I-J-UI-Y-19-9-7----~-------~~-------------------. 5.

  2. The ESA/ESO/NASA Photoshop fits Liberator Version 2

    Science.gov (United States)

    Christensen, L. L.; Nielsen, L. H.; Nielsen, K. K.; Johansen, T.

    2005-12-01

    Since the fi rst version of the FITS Liberator software was released on the 8th July 2004 more than 50,000 people worldwide have looked over the scientists' shoulders and worked with digital images from telescopes in space and on the ground themselves. In this way the Liberator has become the `industry standard' for the production of astronomical colour images. The new version 2 of the software has recently been released and opens up a signifi cant number of new possibilities.

  3. Generalized ESO and Predictive Control Based Robust Autopilot Design

    Directory of Open Access Journals (Sweden)

    Bhavnesh Panchal

    2016-01-01

    Full Text Available A novel continuous time predictive control and generalized extended state observer (GESO based acceleration tracking pitch autopilot design is proposed for a tail controlled, skid-to-turn tactical missile. As the dynamics of missile are significantly uncertain with mismatched uncertainty, GESO is employed to estimate the state and uncertainty in an integrated manner. The estimates are used to meet the requirement of state and to robustify the output tracking predictive controller designed for nominal system. Closed loop stability for the controller-observer structure is established. An important feature of the proposed design is that it does not require any specific information about the uncertainty. Also the predictive control design yields the feedback control gain and disturbance compensation gain simultaneously. Effectiveness of GESO in estimation of the states and uncertainties and in robustifying the predictive controller in the presence of parametric uncertainties, external disturbances, unmodeled dynamics, and measurement noise is illustrated by simulation.

  4. Los prejuicios en una muestra de alumnos de la ESO

    OpenAIRE

    Alonso Martín, Pilar; Lobato Soriano, Herminia

    2004-01-01

    El prejuicio puede ser considerado como una actitud constituida por tres elementos: juicios previos sin una base cognitiva suficiente de información (estereotipos), un componente afectivo y un componente conductual. Este estudio pretende describir las actitudes que tienen los escolares de los cuatro cursos de la Educación Secundaria Obligatoria de un colegio concertado respecto a cuatro temas: raza, inmigrantes, la incorporación laboral de la mujer y la homosexualidad. Para ello se pasó un cu...

  5. Groupement des anciens du CERN et de l'ESO

    CERN Multimedia

    GAC-EPA

    2011-01-01

    Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le Mardi 8 mars de 13h30 à 16h00 Salle de conférence de l’Association du personnel Les permanences suivantes auront lieu les mardis 13 avril, 4 mai, 1er juin, 7 septembre, 5 octobre, 2 novembre et 7 décembre. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants !) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. * * * * * Le Comité du GAC-EPA vous informe de la tenue de son Assemblée générale ordinaire 2011 Vendredi 25 mars 2011 à 14h00 dans l’Amphithé&...

  6. Groupement des anciens du CERN et de l'ESO

    CERN Multimedia

    GAC-EPA

    2011-01-01

    Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le Mardi 13 avril de 13h30 à 16h00 Salle de conférence de l’Association du personnel Les permanences suivantes auront lieu les mardis 4 mai, 1er juin, 7 septembre, 5 octobre, 2 novembre et 7 décembre. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants !) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. * * * * * Le Comité du GAC-EPA vous informe de la tenue de son Assemblée générale ordinaire 2011 Vendredi 25 mars 2011 à 14h00 dans l’Amphith&e...

  7. ESO65-SC03, Teutsch 106, Turner 6

    Indian Academy of Sciences (India)

    GIREESH C. JOSHI

    2017-11-27

    Nov 27, 2017 ... of each cluster is estimated through the visual inspection of best fitted isochrone in colour magnitude diagrams. (CMDs). The mean ... and two colour magnitude ratio (TCMR) values of each cluster is determined by utilizing the WISE and PPMXL catalogues ... enhanced compared to the nearby sky. They are ...

  8. Educación en emprendimiento en la ESO

    OpenAIRE

    Valverde-Bros, Manuel

    2013-01-01

    Se puede enseñar a emprender. Sobre esta convicción se fundamentará el trabajo que se presenta a continuación. La necesidad de fomentar el espíritu emprendedor desde la educación es una respuesta unánime que aparece en todas las directivas educativas con perspectiva de futuro. Se busca formar a personas maduras que, no tan sólo posean conocimientos sino que sean capaces de integrarse fácilmente en la sociedad como ciudadanos responsables y libres. Para ello deben ser capace...

  9. The Innsbruck/ESO sky models and telluric correction tools*

    Directory of Open Access Journals (Sweden)

    Kimeswenger S.

    2015-01-01

    While the ground based astronomical observatories just have to correct for the line-of-sight integral of these effects, the Čerenkov telescopes use the atmosphere as the primary detector. The measured radiation originates at lower altitudes and does not pass through the entire atmosphere. Thus, a decent knowledge of the profile of the atmosphere at any time is required. The latter cannot be achieved by photometric measurements of stellar sources. We show here the capabilities of our sky background model and data reduction tools for ground-based optical/infrared telescopes. Furthermore, we discuss the feasibility of monitoring the atmosphere above any observing site, and thus, the possible application of the method for Čerenkov telescopes.

  10. Address by Catherine Cesarsky, Director General of ESO

    Science.gov (United States)

    2003-12-01

    This is a great day for Astronomy. This is indeed a great day for Chile, for the II Region and for San Pedro de Atacama. And this is indeed a great day for all of us, a moment to which we have all been looking forward with great anticipations. This is the real beginning of a joint adventure. We will be reaching towards the stars, searching for the earliest, remotest objects in the Universe, peering beyond current horizons into the deep unknown.

  11. ¿Qué es eso que llaman ciencia?

    OpenAIRE

    Jaramillo Antillón, Juan

    2011-01-01

    INTRODUCCION: P: La gente encuentra que la ciencia es difícil de aprender y difícil de enseñar. R: Por qué, por siglos, los científicos no trataron de explicar en forma sencilla sus descubrimientos y sus implicaciones para la sociedad, era un grupo muy cerrado. En los últimos 50 años se ha tratado de popularizar la ciencia en periódicos, revistas, la televisión e Internet, dando a conocer los descubrimientos científicos (en especial en el campo de la medicina) y la importancia de ellos

  12. humano. Un estudio en 3º de ESO

    Directory of Open Access Journals (Sweden)

    Jesús Ramón Girón Gambero

    2015-01-01

    alimentos. La investigación se ha llevado a cabo con el objetivo de estudiar el desempeño del alumnado en elanálisis de un video publicitario en un contexto real de aula, mediante un estudio de caso en 3º de EducaciónSecundaria Obligatoria. Se utiliza un anuncio televisivo que incluye un modelo para explicar cómo actúa unconocido producto probiótico en el organismo. Para obtener datos y evidencias se han utilizado el diario delprofesor, grabaciones en vídeo de las clases y los cuadernos de trabajo de los estudiantes. En los resultados sedestacan los elementos más fácilmente interpretables y las dificultades del alumnado para comprender el modelodel anuncio. Finalmente, como implicación educativa, se formulan algunas ideas que podrían formar parte de un modelo escolar que explique cómo actúan las defensas en el intestino humano, integrando aquellos aspectos positivos identificados en el anuncio con algunos elementos de los propios modelos de los alumnos y las ideas científicas al respecto.

  13. VizieR Online Data Catalog: The ESO DIBs Large Exploration Survey (Cox+, 2017)

    Science.gov (United States)

    Cox, N. L. J.; Cami, J.; Farhang, A.; Smoker, J.; Monreal-Ibero, A.; Lallement, R.; Sarre, P. J.; Marshall, C. C. M.; Smith, K. T.; Evans, C. J.; Royer, P.; Linnartz, H.; Cordiner, M. A.; Joblin, C.; van Loon, J. T.; Foing, B. H.; Bhatt, N. H.; Bron, E.; Elyajouri, M.; de Koter, A.; Ehrenfreund, P.; Javadi, A.; Kaper, L.; Khosroshadi, H. G.; Laverick, M.; Le Petit, F.; Mulas, G.; Roueff, E.; Salama, F.; Spaans, M.

    2018-01-01

    We constructed a statistically representative survey sample that probes a wide range of interstellar environment parameters including reddening E(B-V), visual extinction AV, total-to-selective extinction ratio RV, and molecular hydrogen fraction fH2. EDIBLES provides the community with optical (~305-1042nm) spectra at high spectral resolution (R~70000 in the blue arm and 100000 in the red arm) and high signal-to-noise (S/N; median value ~500-1000), for a statistically significant sample of interstellar sightlines. Many of the >100 sightlines included in the survey already have auxiliary available ultraviolet, infrared and/or polarisation data on the dust and gas components. (2 data files).

  14. ¿Y eso cómo se come? La interculturalidad y las comidas familiares

    Directory of Open Access Journals (Sweden)

    Francisco Adame Gómez

    2015-10-01

    Full Text Available Las comidas familiares son un ritual común a muchas culturas pero único en cómo se parametriza en cada una de ellas. Cada una se rige por unas normas no explícitas que todos los comensales, al compartir la misma cultura, conocen. Sin embargo, cuando alguien de una cultura diferente participa en una comida de este tipo, pueden producirse malentendidos y choques culturales fruto del desconocimiento de esas normas. Estas situaciones incómodas pueden solucionarse trabajando la interculturalidad en el aula de E/LE. La actividad que proponemos parte de una reflexión sobre la cultura propia para compararla con la cultura meta, seguida de una divertida simulación en la que nuestros alumnos tendrán que ponerse en la piel de un español. Tiene una duración de unos 120 minutos y está pensada para el nivel B2, aunque es adaptable a cualquier otro nivel.

  15. Libros que son eso, libros, y no son, ni remotamente, productos literarios

    Directory of Open Access Journals (Sweden)

    Julio Daniel Chaparro H.

    1990-01-01

    Full Text Available La tragedia de Armero. Augusto Cardona Agudelo. Ediciones Pijao, Bogotá, 1988, 127 págs. El último mártir de las bananeras. Miguel Madrid García. Ediciones Pijao, Bogotá, 1988, 55 págs. La muerte no respeta banderas. Antonio Echeverry Gil. Ediciones Pijao, Bogotá, 1988, 126 págs. Los oficios del hambre. Miguel Torres. Editorial Tiempo Presente, Bogotá, 1988, 126 págs. Los duros de la salsa también bailan bolero. Laureano Alba. Secretaría de Educación, Cultura y Recreación de Cali, Cali, 1987, 154 págs. Jepira. José Soto. Arango Editoriales, Bogotá, 1989, 169 págs.

  16. The Gaia-ESO Survey: the Galactic thick to thin disc transition

    NARCIS (Netherlands)

    Recio-Blanco, A.; de Laverny, P.; Kordopatis, G.; Helmi, A.; Hill, V.; Gilmore, G.; Wyse, R.; Adibekyan, V.; Randich, S.; Asplund, M.; Feltzing, S.; Jeffries, R.; Micela, G.; Vallenari, A.; Alfaro, E.; Allende Prieto, C.; Bensby, T.; Bragaglia, A.; Flaccomio, E.; Koposov, S. E.; Korn, A.; Lanzafame, A.; Pancino, E.; Smiljanic, R.; Jackson, R.; Lewis, J.; Magrini, L.; Morbidelli, L.; Prisinzano, L.; Sacco, G.; Worley, C. C.; Hourihane, A.; Bergemann, M.; Costado, M. T.; Heiter, U.; Joffre, P.; Lardo, C.; Lind, K.; Maiorca, E.

    Aims: The nature of the thick disc and its relation to the thin disc is presently an important subject of debate. In fact, the structural and chemo-dynamical transition between disc populations can be used as a test of the proposed models of Galactic disc formation and evolution. Methods: We used

  17. El área de Música en la ESO: variables motivacionales de los estudiantes

    OpenAIRE

    Iborra Muñiz, José Germán

    2015-01-01

    El objetivo general de este trabajo es indagar sobre las metas académicas, las dimensiones de las atribuciones causales, las dimensiones del autoconcepto, las metas sociales y las percepciones de éxito del alumnado en el área de Música, así como analizar las relaciones que se producen entre estas variables y el papel que juegan para predecir el rendimiento académico. Partiendo de la idea de que la motivación es “un estado interno que activa, dirige y mantiene el comportamiento” (Woolfolk, 201...

  18. VizieR Online Data Catalog: Gaia-ESO Survey. Trumpler 23 (Overbeek+, 2017)

    Science.gov (United States)

    Overbeek, J. C.; Friel, E. D.; Donati, P.; Smiljanic, R.; Jacobson, H. R.; Hatzidimitriou, D.; Held, E. V.; Magrini, L.; Bragaglia, A.; Randich, S.; Vallenari, A.; Cantat-Gaudin, T.; Tautvaisiene, G.; Jimenez-Esteban, F.; Frasca, A.; Geisler, D.; Villanova, S.; Tang, B.; Munoz, C.; Marconi, G.; Carraro, G.; San, Roman I.; Drazdauskas, A.; Zenoviene, R.; Gilmore, G.; Jeffries, R. D.; Flaccomio, E.; Pancino, E.; Bayo, A.; Costado, M. T.; Damiani, F.; Jofre, P.; Monaco, L.; Prisinzano, L.; Sousa, S. G.; Zaggia, S.

    2017-05-01

    Data for GES are taken with the Fiber Large Array Multi-Element Spectrograph (FLAMES) on the VLT at the European Southern Observatory. FLAMES has two instruments, the medium-resolution multi-object spectrograph GIRAFFE and the high-resolution Ultraviolet and Visual Echelle Spectrograph (UVES). For intermediate-age OCs with prominent red clumps, GES targets are selected as follows: likely clump stars are observed with UVES, so that the most time-intensive targets are most likely to be members, followed by probable red giants if the clump is sparse. Main sequence stars down to V=19 are observed with GIRAFFE, using the HR9B setup primarily for stars of spectral type A to F and the HR15N setup for cooler stars. General GES target selection methods are outlined in Bragaglia et al. (in prep.). (6 data files).

  19. ESO and Euro3D Workshop on Science Perspectives for 3D Spectroscopy

    CERN Document Server

    Kissler-Patig, Markus; Roth, Martin M; ESO Workshop

    2007-01-01

    This volume contains the proceedings of the last conference ever on integral-field spectroscopy. A daring statement, indicating that integral-field spectroscopy has evolved into a mature technique - a common user utility for astronomical research. Nowadays many integral-field spectrographs are installed on 4m to 8-10m class telescopes around the world. While many of those instruments are referred to in this volume, the book is explicitly not dedicated to technical issues, but is focusing on the scientific questions that can be answered with integral-field spectroscopy. These range from solar system studies all the way to high redshift surveys.

  20. Las mujeres que aspiran y eso de la ortografía

    Directory of Open Access Journals (Sweden)

    Hernán Rodríguez Castelo

    2015-01-01

    Full Text Available El autor inicia refiriéndose al mal uso de la "a" por los periodistas. Luego se pronuncia por los tres campos en la ortografía española que generan problemas: las tildes, las mayúsculas y las letras. Hace un análisis de los cambios que propuso Gabriel García Marqués y agrega varios comentarios de cultores de la lengua español.

  1. La transversalidad y los valores de las matemáticas de la ESO

    OpenAIRE

    Bejarano, Antonio

    1997-01-01

    La preocupación por los valores es algo común a los sistemas educativos de nuestro entorno. En nuestro país, la introducción explícita, en el nuevo sistema educativo, de contenidos que hacen referencia a valores, actitudes y normas en cada una de las áreas y, posteriormente, la propuesta de los siete temas transversales nos indica la gran importancia que dedica a este tema el proyecto curricular que emana de la LOGSE.

  2. Report on the ESO and Excellence Cluster Universe Workshop "Galaxy Ecosystem: Flow of Baryons through Galaxies"

    Science.gov (United States)

    Mainieri, V.; Popesso, P.

    2017-12-01

    This conference focussed on the "baryon cycle", namely the flow of baryons through galaxies. The following aspects were discussed: a) the gas inflow into systems through streams of pristine gas or as drizzles of recycled material; b) the conversion of this gas into stars; and c) the ejection of gas enriched with heavy elements through powerful outflows. Understanding these different but mutually connected phases is of fundamental importance when studying the details of galaxy formation and evolution through cosmic time. This conference was held following the month-long workshop of the Munich Institute for Astro- and Particle Physics (MIAPP) entitled: "In & out: What rules the galaxy baryon cycle?" It therefore provided an opportunity to share the main outcomes of the MIAPP workshop with a larger audience, including many young outstanding scientists who could not attend the MIAPP workshop.

  3. Report on the ESO Workshop "Early Stages of Galaxy Cluster Formation 2017 (GCF2017)"

    Science.gov (United States)

    Mroczkowski, T.; Stroe, A.; Andreani, P.; Arnaud, M.; Arrigoni Battaia, F..; De Breuck, C..; Sobral, D.

    2017-12-01

    The formation of the largest gravitationally bound structures in the Universe, clusters of galaxies, and how these environments affect the galaxies within them are major themes in cosmology and galaxy evolution. The high-redshift progenitors of clusters, called "protoclusters", are still in the process of hierarchical assembly. The transition from protocluster to cluster is gradual, driven by accretion and spectacular mergers that are expected to last roughly one billion years. This workshop aimed to address open questions in protocluster and cluster formation, to define the similarities and distinctions between the two, and to evaluate the best tools and methods for their detection and study.

  4. Treatment outcome in early diffuse cutaneous systemic sclerosis: the European Scleroderma Observational Study (ESOS)

    Science.gov (United States)

    Herrick, Ariane L; Pan, Xiaoyan; Peytrignet, Sébastien; Lunt, Mark; Hesselstrand, Roger; Mouthon, Luc; Silman, Alan; Brown, Edith; Czirják, László; Distler, Jörg H W; Distler, Oliver; Fligelstone, Kim; Gregory, William J; Ochiel, Rachel; Vonk, Madelon; Ancuţa, Codrina; Ong, Voon H; Farge, Dominique; Hudson, Marie; Matucci-Cerinic, Marco; Balbir-Gurman, Alexandra; Midtvedt, Øyvind; Jordan, Alison C; Jobanputra, Paresh; Stevens, Wendy; Moinzadeh, Pia; Hall, Frances C; Agard, Christian; Anderson, Marina E; Diot, Elisabeth; Madhok, Rajan; Akil, Mohammed; Buch, Maya H; Chung, Lorinda; Damjanov, Nemanja; Gunawardena, Harsha; Lanyon, Peter; Ahmad, Yasmeen; Chakravarty, Kuntal; Jacobsen, Søren; MacGregor, Alexander J; McHugh, Neil; Müller-Ladner, Ulf; Riemekasten, Gabriela; Becker, Michael; Roddy, Janet; Carreira, Patricia E; Fauchais, Anne Laure; Hachulla, Eric; Hamilton, Jennifer; İnanç, Murat; McLaren, John S; van Laar, Jacob M; Pathare, Sanjay; Proudman, Susannah; Rudin, Anna; Sahhar, Joanne; Coppere, Brigitte; Serratrice, Christine; Sheeran, Tom; Veale, Douglas J; Grange, Claire; Trad, Georges-Selim; Denton, Christopher P

    2017-01-01

    Objectives The rarity of early diffuse cutaneous systemic sclerosis (dcSSc) makes randomised controlled trials very difficult. We aimed to use an observational approach to compare effectiveness of currently used treatment approaches. Methods This was a prospective, observational cohort study of early dcSSc (within three years of onset of skin thickening). Clinicians selected one of four protocols for each patient: methotrexate, mycophenolate mofetil (MMF), cyclophosphamide or ‘no immunosuppressant’. Patients were assessed three-monthly for up to 24 months. The primary outcome was the change in modified Rodnan skin score (mRSS). Confounding by indication at baseline was accounted for using inverse probability of treatment (IPT) weights. As a secondary outcome, an IPT-weighted Cox model was used to test for differences in survival. Results Of 326 patients recruited from 50 centres, 65 were prescribed methotrexate, 118 MMF, 87 cyclophosphamide and 56 no immunosuppressant. 276 (84.7%) patients completed 12 and 234 (71.7%) 24 months follow-up (or reached last visit date). There were statistically significant reductions in mRSS at 12 months in all groups: −4.0 (−5.2 to −2.7) units for methotrexate, −4.1 (−5.3 to −2.9) for MMF, −3.3 (−4.9 to −1.7) for cyclophosphamide and −2.2 (−4.0 to −0.3) for no immunosuppressant (p value for between-group differences=0.346). There were no statistically significant differences in survival between protocols before (p=0.389) or after weighting (p=0.440), but survival was poorest in the no immunosuppressant group (84.0%) at 24 months. Conclusions These findings may support using immunosuppressants for early dcSSc but suggest that overall benefit is modest over 12 months and that better treatments are needed. Trial registration number NCT02339441. PMID:28188239

  5. Diversidad y geometría en la ESO : el caso de alumnado deficiente auditivo

    Directory of Open Access Journals (Sweden)

    Núria Rosich Sala

    2013-05-01

    Full Text Available En la investigación que estamos realizando en el proyecto AUDIMAT desde 2001, queremos reconocer y evaluar las dificultades de alumnado con deficiencia auditiva incluido en clases regulares con la intención de conseguir un aprendizaje matemático (geométrico que tenga los mismos objetivos que los que proponemos para sus pares oyentes. A partir del diseño de un módulo de aprendizaje a distancia, se comparan los razonamientos realizados por los deficientes auditivos con los de los pares oyentes, en cuanto la visualización y medida de volumen en distintos momentos de un proceso de regulación.

  6. Heritability of Strabismus : Genetic Influence Is Specific to Eso-Deviation and Independent of Refractive Error

    NARCIS (Netherlands)

    Sanfilippo, P.G.; Hammond, C.J.; Staffieri, S.E.; Kearns, L.S.; Liew, S.H.M.; Barbour, J.M.; Hewitt, A.W.; Ge, D.L.; Snieder, H.; MacKinnon, J.R.; Brown, S.A.; Lorenz, B.; Spector, T.D.; Martin, N.G.; Wilmer, J.B.; Mackeymo, D.A.

    2012-01-01

    Strabismus represents a complex oculomotor disorder characterized by the deviation of one or both eyes and poor vision. A more sophisticated understanding of the genetic liability of strabismus is required to guide searches for associated molecular variants. In this classical twin study of 1,462

  7. Possible astronomical meanings of some El Molle relics near the ESO Observatory at La Silla

    Science.gov (United States)

    Bernardi, Gabriella; Vecchiato, Alberto; Bucciarelli, Beatrice

    2012-07-01

    This paper describes a peculiar, man-made circular stone structure, associated with the ancient rock engravings that are around the site of La Silla in Chile close to the European Southern Observatory, and are attributed to the El Molle Culture. Three stones of the circle, different from all the others, were likely to pinpoint the alignment of three bright stars close to the horizon, as seen from a specific vantage point inside the structure. The El Molle was the only period in which this alignment occurred significantly close to the horizon, moreover it was only in this epoch that it could also be associated with the transition from the warm to the cold season, a period of the year which was quite important for a society that supported itself by herding and farming.

  8. ESO Diffuse Interstellar Bands Large Exploration Survey (EDIBLES) - Merging Observations and Laboratory Data

    Science.gov (United States)

    Salama, Farid

    2016-01-01

    The Diffuse Interstellar Bands (DIBs) are a set of 500 absorption bands that are detected in the spectra of stars with interstellar clouds in the line of sight. DIBs are found from the NUV to the NIR in the spectra of reddened stars spanning different interstellar environments in our local, and in other galaxies. DIB carriers are a significant part of the interstellar chemical inventory. They are stable and ubiquitous in a broad variety of environments and play a unique role in interstellar physics/chemistry. It has long been realized that the solving of the DIB problem requires a strong synergy between astronomical observations, laboratory astrophysics, and astrophysical modeling of line-of-sights. PAHs are among the molecular species that have been proposed as DIB carriers. We will present an assessment of the PAH-DIB model in view of the progress and the advances that have been achieved over the past years through a series of studies involving astronomical observations of DIBs, laboratory simulation of interstellar analogs for neutrals and ionized PAHs, theoretical calculations of PAH spectra and the modelization of diffuse and translucent interstellar clouds. We will present a summary of what has been learned from these complementary studies, the constraints that can now be derived for the PAHs as DIB carriers in the context of the PAH-DIB model and how these constraints can be applied to the EDIBLES project. The spectra of several neutral and ionized PAHs isolated in the gas phase at low temperature have been measured in the laboratory under experimental conditions that mimic interstellar conditions and are compared with an extensive set of astronomical spectra of reddened, early type stars. The comparisons of astronomical and laboratory data provide upper limits for the abundances of specific neutral PAH molecules and ions along specific lines-of-sight. Something that is not attainable from infrared observations alone. We present the characteristics of the laboratory facilities, MIS and COSmIC, that have been developed for this study and discuss the findings resulting from the comparison of the laboratory data with high resolution, high S/N ratio astronomical observations. MIS stands for Matrix Isolation Spectroscopy, a well-proven technique for isolating cold molecular species in inert solid environments. COSmIC stands for Cosmic Simulation Chamber. It combines a supersonic free jet expansion with discharge plasma and high-sensitivity cavity ringdown spectroscopy and time-of-flight mass spectrometry detection tools for the generation and the detection of cold, isolated gas-phase molecules and ions under experimental conditions that closely mimic interstellar conditions. The column densities of the individual neutral PAH molecules and ions probed in these surveys are derived from the comparison of these unique laboratory data with high resolution, high S/N ratio astronomical observations. The comparisons of astronomical and laboratory data lead to clear and unambiguous conclusions regarding the expected abundances for PAHs of various sizes and charge states in the interstellar environments probed in the surveys. Band profile comparisons between laboratory and astronomical spectra lead to information regarding the molecular structures and characteristics associated with the DIB carriers in the corresponding lines-of-sight. These quantitative surveys of neutral and ionized PAHs in the optical range open the way for unambiguous quantitative searches of PAHs and complex organics in a variety of interstellar and circumstellar environments.

  9. ESO and the UK. Why does the UK need more astronomy?

    Science.gov (United States)

    Gilmore, Gerry

    2002-09-01

    “What was God doing before he made heaven and earth? … He was preparing hell for those who would pry into such profound mysteries.''1 This joke was already venerable when quoted by Augustine, in his analysis of the ancient and still modern problem, time. Understanding the origin(s), meaning( s), future(s), and significance(s) of time, space, existence, mass, matter, geometry, of origins and endings, of what and where, remains one of the greatest intellectual endeavours of the human mind. From the caves of Lascaux, through the megaliths of Stonehenge to the dreamtime of Australia, mankind has striven to understand his origins and future. Our generation has the exceptional good fortune to be living through the greatest increase in knowledge relevant to these fundamental questions since someone first looked up at night. We are also increasing understanding, while realizing how much more there is in the Universe still be learned and understood.

  10. Autonomy and responsibility as key values for the elaboration of rules of coexistence in the ESO

    Directory of Open Access Journals (Sweden)

    Cruz Pérez Pérez

    2012-07-01

    Full Text Available Normal.dotm 0 0 1 150 860 Universidad de Salamanca 7 1 1056 12.0 0 false 18 pt 18 pt 0 0 false false false /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} Some of the themes set out in the article are the following: are pupils capable of organising behaviour and coexistence in the classroom? Are they able to elaborate their own rules? Or is it only a theoretical approach that is unviable to put into practise? In any case, what level of responsibility and autonomy are they able to assume in the functioning of the classroom? The learning of rules in the classroom constitutes a real experience, very close to the experience and the interests of the pupils, that permits them to work on values such as: responsibility, respect, participation, dialogue and democracy. At the same time, it teaches them to participate in class meetings, work in small groups, formulate proposals and arrive to a consensus through dialogue and negotiation. The experimental research in this field has demonstrated on numerous occasions that when the teacher generates opportunities to reflect on coexistence in the classroom and involve their pupils, it produces a clear tendency to decrease disruptive behaviour.

  11. Identification of Stellar Sequences in Various Stellar Systems: ESO65-SC03, Teutsch 106, Turner 6

    Science.gov (United States)

    Joshi, Gireesh C.

    2017-12-01

    The spatial morphological study of stellar clusters has been carried out through their identified probable members. The field stars decontamination is performed by the statistical cleaning approach (depends on the magnitude and colour of stars within the field and cluster regions). The colour magnitude ratio diagram (CMRD) approach is used to separate the stellar sequences of cluster systems. The age, distance and reddening of each cluster is estimated through the visual inspection of best fitted isochrone in colour magnitude diagrams (CMDs). The mean proper motion values of stellar clusters are obtained through the extracted data from PPMXL and UCAC4 catalogs. Moreover, these values vary according to the extracted data-set from these catalogues. This variation has occurred due to different estimation efficiency of proper motions. The two colour ratio (TCR) and two colour magnitude ratio (TCMR) values of each cluster is determined by utilizing the WISE and PPMXL catalogues, these values are found abnormal for Teutsch 106. In addition, the TCMR values are similar to TCR values at longer wavelength, whereas both values are far away from each other at shorter wavelength. The fraction of young stellar objects (YSOs) is also computed for each cluster.

  12. ALUMNADO INMIGRANTE EN LA ESO: VULNERABILIDAD PEDAGÓGICA DEL SISTEMA EDUCATIVO

    Directory of Open Access Journals (Sweden)

    Juan Fernández Sierra

    2017-01-01

    Full Text Available Es preocupante el alto porcentaje de alumnado inmigrante que no culmina con éxito la enseñanza obligatoria, extendiéndose una tendencia de pensamiento docente que identifica las causas con cuestiones de índole personal, sociofamiliar o cultural. Sin embargo, parece razonable indagar en qué yerra nuestro sistema educativo que no logra superar esa situación. En esa línea, el objetivo esencial de esta investigación ha sido indagar en los puntos débiles o vulnerabilidad del sistema (Reimer, 1973 para responder a este escenario, enfocándolo desde la perspectiva de los planteamientos de política curricular y examinando las raíces institucionales y pedagógicas que inciden en la deserción y fracaso escolar. Para ello, hemos realizado un diseño de corte naturalista basado en ocho estudios instrumentales de caso, llevados a cabo en otros tantos institutos de diversas características, desarrollándolos al modo de lo que Stake (2007 denomina estudio colectivo de casos. En cuanto a los resultados y conclusiones, damos especial relevancia al tándem formado por la que podríamos denominar vulnerabilidad intrínseca —actitudes y prácticas hegemónicas mediatizadas por la cultura profesional instalada en este nivel educativo— y la vulnerabilidad estructural —políticas educativas y curriculares—, que se retroalimentan mutuamente, construyendo una resistencia simbólica, normativa y de práctica pedagógica, inhibidora de los intentos innovadores para reorientar la acción educativa hacia dimensiones más inclusivas, multiculturales e interculturales. Los estereotipos y la cultura académica previa experimentada por las familias y prole, también se muestran como factores significativos de vulnerabilidad. El diseño empleado, por su propia naturaleza, no permite generalizar los hallazgos, pero sí posee un alto poder de transferibilidad y de aportación de interpretaciones y asertos para seguir comprendiendo las complejidades de la educación actual e ilustrar la toma de decisiones políticas y prácticas en este ámbito.

  13. Mejora de la conducta disruptiva en un aula de Matemáticas de la ESO

    OpenAIRE

    García López, Pamela

    2016-01-01

    Treball Final del Màster Universitari en Professor/a d'Educació Secundària Obligatòria i Batxillerat, Formació Professional i Ensenyaments d'Idiomes. Codi: SAP509. Curs acadèmic 2015-2016 El presente Trabajo Final de Máster (TFM) está dentro de la modalidad de Mejora Educativa según la tipología y normativa que concreta la Universidad Jaime I. Según esta modalidad, el trabajo consiste en una experiencia de investigación durante el prácticum del Máster en el IES Profesor Broch i Ll...

  14. GENIE: a ground-based European nulling instrument at ESO very large telescope interferometer

    NARCIS (Netherlands)

    Gondoin, P.; Hartog, R. den; Fridlund, M.; Fabry, P.; Stankov, A.; Peacock, A.; Volonte, S.; Puech, F.; Delplancke, F.; Gitton, P.; Glindemann, A.; Paresce, F.; Richichi, A.; Barillot, M.; Absil, O.; Cassaing, F.; Foresto, V.C. du; Kervella, P.; Perrin, G.; Ruilier, C.; Flatscher, R.; Bokhove, H.; Ergenzinger, K.; Quirrenbach, A.; Wallner, O.; Alves, J.; Herbst, T.; Mourard, D.; Neuhäuser, R.; Ségransan, D.; Waters, R.; White, G.J.

    2008-01-01

    Darwin is one of the most challenging space projects ever considered by the European Space Agency (ESA). Its principal objectives are to detect Earth-like planets around nearby stars, to analyze the composition of their atmospheres and to assess their ability to sustain life as we know it. Darwin is

  15. Autonomy and responsibility as key values for the elaboration of rules of coexistence in the ESO Autonomía y responsabilidad como valores clave para la elaboración de normas de convivencia en la ESO

    Directory of Open Access Journals (Sweden)

    Cruz Pérez Pérez

    2012-07-01

    Full Text Available Normal.dotm 0 0 1 150 860 Universidad de Salamanca 7 1 1056 12.0 0 false 18 pt 18 pt 0 0 false false false /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} Some of the themes set out in the article are the following: are pupils capable of organising behaviour and coexistence in the classroom? Are they able to elaborate their own rules? Or is it only a theoretical approach that is unviable to put into practise? In any case, what level of responsibility and autonomy are they able to assume in the functioning of the classroom? The learning of rules in the classroom constitutes a real experience, very close to the experience and the interests of the pupils, that permits them to work on values such as: responsibility, respect, participation, dialogue and democracy. At the same time, it teaches them to participate in class meetings, work in small groups, formulate proposals and arrive to a consensus through dialogue and negotiation. The experimental research in this field has demonstrated on numerous occasions that when the teacher generates opportunities to reflect on coexistence in the classroom and involve their pupils, it produces a clear tendency to decrease disruptive behaviour. Normal.dotm 0 0 1 160 917 Universidad de Salamanca 7 1 1126 12.0 0 false 18 pt 18 pt 0 0 false false false /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} Algunas de las cuestiones que se plantean en el artículo son las siguientes: ¿Son los alumnos capaces de organizar el comportamiento y la convivencia en el aula? ¿Están capacitados para elaborar sus propias normas? o ¿Solamente se trata de un planteamiento teórico que resulta inviable de llevar a la práctica? En todo caso, ¿Qué nivel de responsabilidad y autonomía pueden asumir en el funcionamiento del aula?   El aprendizaje de normas en el aula constituye una experiencia real, muy próxima a la experiencia y los intereses de los alumnos, que permite trabajar valores como la responsabilidad, el respeto, la participación, el diálogo y la democracia. A su vez les enseña a participar en asambleas de aula, a trabajar en pequeños grupos, a formular propuestas y a llegar al consenso a través del diálogo y la negociación. La investigación experimental en este campo ha demostrado en múltiples ocasiones que cuando el docente genera oportunidades de reflexión sobre la convivencia en el aula e involucra a sus alumnos en la misma, se produce una clara tendencia a la disminución de las conductas disruptivas.

  16. Combining Dark Energy Survey Science Verification data with near-infrared data from the ESO VISTA Hemisphere Survey

    Energy Technology Data Exchange (ETDEWEB)

    Banerji, M.; Jouvel, S.; Lin, H.; McMahon, R. G.; Lahav, O.; Castander, F. J.; Abdalla, F. B.; Bertin, E.; Bosman, S. E.; Carnero, A.; Kind, M. C.; da Costa, L. N.; Gerdes, D.; Gschwend, J.; Lima, M.; Maia, M. A. G.; Merson, A.; Miller, C.; Ogando, R.; Pellegrini, P.; Reed, S.; Saglia, R.; Sanchez, C.; Allam, S.; Annis, J.; Bernstein, G.; Bernstein, J.; Bernstein, R.; Capozzi, D.; Childress, M.; Cunha, C. E.; Davis, T. M.; DePoy, D. L.; Desai, S.; Diehl, H. T.; Doel, P.; Findlay, J.; Finley, D. A.; Flaugher, B.; Frieman, J.; Gaztanaga, E.; Glazebrook, K.; Gonzalez-Fernandez, C.; Gonzalez-Solares, E.; Honscheid, K.; Irwin, M. J.; Jarvis, M. J.; Kim, A.; Koposov, S.; Kuehn, K.; Kupcu-Yoldas, A.; Lagattuta, D.; Lewis, J. R.; Lidman, C.; Makler, M.; Marriner, J.; Marshall, J. L.; Miquel, R.; Mohr, J. J.; Neilsen, E.; Peoples, J.; Sako, M.; Sanchez, E.; Scarpine, V.; Schindler, R.; Schubnell, M.; Sevilla, I.; Sharp, R.; Soares-Santos, M.; Swanson, M. E. C.; Tarle, G.; Thaler, J.; Tucker, D.; Uddin, S. A.; Wechsler, R.; Wester, W.; Yuan, F.; Zuntz, J.

    2014-11-25

    We present the combination of optical data from the Science Verification phase of the Dark Energy Survey (DES) with near-infrared (NIR) data from the European Southern Observatory VISTA Hemisphere Survey (VHS). The deep optical detections from DES are used to extract fluxes and associated errors from the shallower VHS data. Joint seven-band (grizYJK) photometric catalogues are produced in a single 3 sq-deg dedicated camera field centred at 02h26m-04d36m where the availability of ancillary multiwavelength photometry and spectroscopy allows us to test the data quality. Dual photometry increases the number of DES galaxies with measured VHS fluxes by a factor of ~4.5 relative to a simple catalogue level matching and results in a ~1.5 mag increase in the 80 per cent completeness limit of the NIR data. Almost 70 per cent of DES sources have useful NIR flux measurements in this initial catalogue. Photometric redshifts are estimated for a subset of galaxies with spectroscopic redshifts and initial results, although currently limited by small number statistics, indicate that the VHS data can help reduce the photometric redshift scatter at both z < 0.5 and z > 1. We present example DES+VHS colour selection criteria for high-redshift luminous red galaxies (LRGs) at z ~ 0.7 as well as luminous quasars. Using spectroscopic observations in this field we show that the additional VHS fluxes enable a cleaner selection of both populations with <10 per cent contamination from galactic stars in the case of spectroscopically confirmed quasars and <0.5 per cent contamination from galactic stars in the case of spectroscopically confirmed LRGs. The combined DES+VHS data set, which will eventually cover almost 5000 sq-deg, will therefore enable a range of new science and be ideally suited for target selection for future wide-field spectroscopic surveys.

  17. El blog como recurso educativo en el aula de Lengua Castellana y Literatura de 4º de ESO

    OpenAIRE

    Martínez-Prieto, José Luis

    2013-01-01

    Este trabajo tiene la finalidad de presentar una propuesta práctica con el diseño de una unidad didáctica para la asignatura de Lengua Castellana y Literatura en la clase de 4º de Educación Secundaria Obligatoria con la novedad del uso de un blog, como hilo conductor. En este sentido, la intención es demostrar la eficacia de la utilización de las Tecnologías de la Información y la Comunicación, en este caso concreto a través de un blog, en el mundo educativo. Un hecho que sirve para acercar l...

  18. Hacia una propuesta didáctica multidisciplinar. Acoso escolar: eso no va conmigo…va con todos

    OpenAIRE

    Mencos Fernández, Estefanía

    2013-01-01

    El presente trabajo recoge una revisión teórica sobre la problemática del acoso escolar, así como un programa de detección e intervención que incluye una serie de estrategias psicoeducativas para la prevención del bullying en Educación Primaria. El mejor tratamiento es la prevención. De ahí, la conveniencia de abordar esta temática desde la escuela (implicando a todos los participantes del proceso educativo) y de analizar con serenidad una situación educativa cada día más presente en nuestro...

  19. Groups of Galaxies in the Nearby Universe Proceedings of the ESO Workshop held at Santiago de Chile

    CERN Document Server

    Saviane, Ivo; Borissova, Jordanka

    2007-01-01

    For every galaxy in the field or in clusters, there are about three galaxies in groups. The Milky Way itself resides in a group, and groups can be found at high redshift. The current generation of 10-m class telescopes and space facilities allows the observation of the members of nearby groups with exquisite detail, and their properties can be correlated with the global properties of their host group. Groups in the local Universe offer us the chance to study galaxies in environments characterized by strong interactions. In the cosmological context, groups trace large-scale structures better than clusters, and the evolution of groups and clusters appears to be related. All these aspects of research on groups of galaxies are summarized in this book written by scientists working in various fields.

  20. Estrategias de aprendizaje, comprensión lectora y rendimiento académico en alumnado de ESO

    OpenAIRE

    García-Guerra, María Azahara

    2013-01-01

    El objetivo principal de este Trabajo Fin de Máster es determinar si existe una relación entre las estrategias de aprendizaje y la comprensión lectora con el desempeño académico en estudiantes de 2º de Educación Secundaria Obligatoria. Otro objetivo fue analizar si existe una relación entre las estrategias de aprendizaje y el desempeño académico con los hábitos de estudio. La metodología de la investigación se basó en administrar diferentes pruebas (ACRA; Inventario de hábitos de estudio, IHE...

  1. Relaciones sociales, agresión y violencia en la E.S.O.: diferencias por sexo y estatus socioeconomico

    OpenAIRE

    Perez Pinto, Maria Trinidad

    2015-01-01

    La tesis tiene como objetivo analizar las relaciones sociales, conductas agresivas y violencia en la educación secundaria obligatoria en función de diferencias de sexo y de estatus socioeconómico (en adelante, SES). La gravedad de los datos actuales que señalan altas tasas de violencia de género y conductas agresivas, así como el escaso número de investigaciones que analiza el papel de la variable SES en estas conductas, justifican la relevancia de esta investigación. La tesis se divide en cu...

  2. X-shooter: UV-to-IR intermediate-resolution high-efficiency spectrograph for the ESO VLT

    NARCIS (Netherlands)

    D'Odorico, S.; Andersen, M.I.; Conconi, P.; De Caprio, V.; Delabre, B.; Di Marcantonio, P.; Dekker, H.; Downing, M.D.; Finger, G.; Groot, P.; Hanenburg, H.H.; Hammer, F.; Horville, D.; Hjorth, J.; Kaper, L.; Klougart, J.; Kjaergaard-Rasmussen, P.; Lizon, J.-L.; Marteaud, M.; Mazzoleni, R.; Michaelsen, N.; Pallavicini, R.; Rigal, F.; Santin, P.; Norup Soerensen, A.; Spano, P.; Venema, L.; Vola, P.; Zerbi, F.M.; Hasinger, G.; Turner, M.J.L.

    2004-01-01

    X-shooter is a single target spectrograph for the Cassegrain focus of one of the VLT UTs. It covers in a single exposure the spectral range from the UV to the H band with a possible extension into part of the K band. It is designed to maximize the sensitivity in this spectral range through the

  3. Near Earth asteroid search and follow-up beyond 22nd magnitude. A pilot program with ESO telescopes

    Czech Academy of Sciences Publication Activity Database

    Boattini, A.; Abramo, G. D.; Scholl, H.; Hainaut, O. R.; Boehnhardt, H.; West, R.; Carpino, M.; Hahn, G.; Michelsen, R.; Forti, G.; Pravec, Petr; Valsecchi, G. B.; Asher, D. J.

    2004-01-01

    Roč. 418, č. 2 (2004), s. 743-750 ISSN 0004-6361 Institutional research plan: CEZ:AV0Z1003909 Keywords : solar system * minor planets * asteroids Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 3.694, year: 2004

  4. La Ausencia de las mujeres en los contenidos de la ESO: una propuesta de inclusión

    OpenAIRE

    López-Navajas, Ana

    2010-01-01

    Un grupo de investigación de la Universitat de València está trabajando en un proyecto cuyo objetivo es crear un instrumento de intervención en la enseñanza que posibilite la inclusión de las mujeres dentro del material didáctico y permita la divulgación de una visión de mundo donde hombres y mujeres hayamos participado. Los estudios de mujeres han aportado luz sobre la participación de las mujeres en la sociedad. Nuestro objetivo es incorporarlas al sistema de enseñanza, proporcionand...

  5. Vaccine Therapy With or Without Sirolimus in Treating Patients With NY-ESO-1 Expressing Solid Tumors

    Science.gov (United States)

    2016-10-03

    Anaplastic Astrocytoma; Anaplastic Oligoastrocytoma; Anaplastic Oligodendroglioma; Estrogen Receptor Negative; Estrogen Receptor Positive; Glioblastoma; Hormone-Resistant Prostate Cancer; Metastatic Prostate Carcinoma; Metastatic Renal Cell Cancer; Recurrent Adult Brain Neoplasm; Recurrent Bladder Carcinoma; Recurrent Breast Carcinoma; Recurrent Colorectal Carcinoma; Recurrent Esophageal Carcinoma; Recurrent Gastric Carcinoma; Recurrent Hepatocellular Carcinoma; Recurrent Lung Carcinoma; Recurrent Melanoma; Recurrent Ovarian Carcinoma; Recurrent Prostate Carcinoma; Recurrent Renal Cell Carcinoma; Recurrent Uterine Corpus Carcinoma; Resectable Hepatocellular Carcinoma; Sarcoma; Stage IA Breast Cancer; Stage IA Ovarian Cancer; Stage IA Uterine Corpus Cancer; Stage IB Breast Cancer; Stage IB Ovarian Cancer; Stage IB Uterine Corpus Cancer; Stage IC Ovarian Cancer; Stage II Uterine Corpus Cancer; Stage IIA Breast Cancer; Stage IIA Lung Carcinoma; Stage IIA Ovarian Cancer; Stage IIB Breast Cancer; Stage IIB Esophageal Cancer; Stage IIB Lung Carcinoma; Stage IIB Ovarian Cancer; Stage IIB Skin Melanoma; Stage IIC Ovarian Cancer; Stage IIC Skin Melanoma; Stage IIIA Breast Cancer; Stage IIIA Esophageal Cancer; Stage IIIA Lung Carcinoma; Stage IIIA Ovarian Cancer; Stage IIIA Skin Melanoma; Stage IIIA Uterine Corpus Cancer; Stage IIIB Breast Cancer; Stage IIIB Esophageal Cancer; Stage IIIB Ovarian Cancer; Stage IIIB Skin Melanoma; Stage IIIB Uterine Corpus Cancer; Stage IIIC Breast Cancer; Stage IIIC Esophageal Cancer; Stage IIIC Ovarian Cancer; Stage IIIC Skin Melanoma; Stage IIIC Uterine Corpus Cancer; Stage IV Bladder Urothelial Carcinoma; Stage IV Esophageal Cancer; Stage IV Ovarian Cancer; Stage IV Prostate Cancer; Stage IV Skin Melanoma; Stage IVA Uterine Corpus Cancer; Stage IVB Uterine Corpus Cancer

  6. Positions of Jupiter, Galilean satellites and Pluto obtained in May 1980 with GPO of the ESO, La Silla

    Science.gov (United States)

    Debehogne, H.; Machado, L. E.; Caldeira, J. F.; Vieira, G. G.; Netto, E. R.

    1981-10-01

    In May 1980 H. Debehogne (ORB) and R. R. de Freitas Mourão (Observatório Nacional, Rio de Janeiro, Brasil) observed Jupiter, galilean satellites and Pluto at the GPO (D = 40 cm, f = 4 m). Measures and reductions were performed at the Observatoire Royal de Belgique and at the Observatório do Valongo with the Ascorecord measuring machines (0.1 μ) and by means of five reference stars on the UNIVAC 1400 (Uccle) and on the Burroughs 6700 (NCE, Núcleo de Computação Eletronica da UFRJ) computers (SAO Catalogue, dependence and Least Squares methods).

  7. Le CERN, L'ESA et L'ESO lancent la "Physique en Scène"

    CERN Multimedia

    CERN Press Office. Geneva

    2000-01-01

    Physics is everywhere. The laws of physics govern the Universe, the Sun, the Earth and even our own lives. In today's rapidly developing society, we are becoming increasingly dependent on high technology - computers, transport, and communication are just some of the key areas that are the result of discoveries by scientists working in physics.

  8. USO DE LAS TIC EN EL ÁREA DE TECNOLOGÍA EN 1º DE ESO

    OpenAIRE

    Jiménez-Moreno, Rosa Ana

    2013-01-01

    Las Tecnologías de la Información y la Comunicación (TIC) son herramientas fundamentales que se utilizan a diario en muchos ámbitos de la vida. Comunicación, trabajo, ocio, y también, en los procesos de enseñanza-aprendizaje. Sin embargo en el ámbito escolar la integración de las TIC se está realizando más lentamente debido a diversos motivos, como la falta de recursos y la falta de tiempo de los docentes para formación en TIC. Se estudiarán trabajos de diversos autores que inciden en que la ...

  9. The ESO Diffuse Interstellar Bands Large Exploration Survey (EDIBLES) . I. Project description, survey sample, and quality assessment

    NARCIS (Netherlands)

    Cox, Nick L. J.; Cami, Jan; Farhang, Amin; Smoker, Jonathan; Monreal-Ibero, Ana; Lallement, Rosine; Sarre, Peter J.; Marshall, Charlotte C. M.; Smith, Keith T.; Evans, Christopher J.; Royer, Pierre; Linnartz, Harold; Cordiner, Martin A.; Joblin, Christine; van Loon, Jacco Th.; Foing, Bernard H.; Bhatt, Neil H.; Bron, Emeric; Elyajouri, Meriem; de Koter, Alex; Ehrenfreund, Pascale; Javadi, Atefeh; Kaper, Lex; Khosroshadi, Habib G.; Laverick, Mike; Le Petit, Franck; Mulas, Giacomo; Roueff, Evelyne; Salama, Farid; Spaans, Marco

    2017-01-01

    The carriers of the diffuse interstellar bands (DIBs) are largely unidentified molecules ubiquitously present in the interstellar medium (ISM). After decades of study, two strong and possibly three weak near-infrared DIBs have recently been attributed to the C60^+ fullerene based on observational

  10. Plurilingual integrative approach: an interdisciplinary education experience to integrate all the languages learned in the ESO stage

    Directory of Open Access Journals (Sweden)

    Cristina Mallol

    2017-07-01

    Full Text Available La optimización del aprendizaje de lenguas es hoy en día una de las preocupaciones pedagógicas más significativas en nuestro país. El principal objetivo de este estudio fue analizar las percepciones de los estudiantes y los docentes en un centro de secundaria donde se aplicó un Enfoque Plurilingüe Integrador (EPI. Se llevó a cabo una investigación acción participativa en la que todos los departamentos lingüísticos del centro trabajaron cooperativamente para implantar un Tratamiento Integrado de Lenguas (TIL. Se recopilaron datos a través de cuestionarios y grupos de discusión, entre otros registros. Los resultados evidenciaron que mediante el aprendizaje global, transversal e interdisciplinar, tanto de lenguas maternas como de segundas lenguas o lenguas extranjeras, los alumnos se sentían más seguros, utilizando como recurso sus experiencias y conocimientos previos. En consecuencia, dichos alumnos estaban más motivados para adquirir las competencias plurilingüe y multicultural, dos competencias genéricas muy relevantes para el futuro desarrollo académico y personal de nuestros estudiantes.

  11. R-ESO--NA-NC-E--I-Fe-b-rU-ar-Y-2-00-6

    Indian Academy of Sciences (India)

    2006-02-02

    Feb 2, 2006 ... ferroin-malonic acid BZ reaction. When the wave is ............... broken at a certain point, a pair of spiral waves. ~ ,...,.. ......, develop at this pOint. ISee article on page 43). _. ICourtesy:Peter Ruoff). - .-. Source:www.ux.his.nol -ruoff/BZ_ Phenomenology. html. Back Cover. [I] David A Huffman. ~-. ~. 11925 -1999).

  12. NY-ESO-1- and survivin-specific T-cell responses in the peripheral blood from patients with glioma

    DEFF Research Database (Denmark)

    Liu, Zhenjiang; Poiret, Thomas; Persson, Oscar

    2018-01-01

    (n = 38 samples) protein expression in tumor specimens. T-cells from peripheral blood were stimulated with TAAs (synthetic peptides) in IL-2 and IL-7, or using a combination of IL-2, IL-15 and IL-21. CD4+ and CD8+ T-cells were tested for antigen-specific proliferation by flow cytometry, and IFN...

  13. La didáctica de las Ciencias Sociales para una alumna con dislexia de 3ºESO

    OpenAIRE

    Arjones-Fernandez, Maria Aurora

    2010-01-01

    La acción educativa que planificamos a través de esta Programación Anual y de Aula, que integran esta Programación Didáctica, se fija en la atención a la diversidad mediante la integración activa de alumnos/as procedentes de contextos culturales diversos, con distintos ritmos de aprendizajes así como contemplamos las necesidades educativas específicas de la deficiencia visual y la dislexia en dos de nuestros alumnos/as. Los avances en Tiflotecnología y su aplicación en centros TICS de la Junt...

  14. Uso de videojuegos con alumnos de 3º de ESO para fomentar la creatividad y el trabajo cooperativo

    OpenAIRE

    Illán-Bernal, Julián

    2013-01-01

    El presente trabajo trata de acercarse al mundo de los videojuegos para conocer, de la mano de tres autores e investigadores en la materia, una faceta hasta ahora desconocida o, al menos, no asumida por la sociedad: el valor pedagógico de los videojuegos. Los videojuegos nos brindan la oportunidad de desarrollar en las aulas dos aspectos clave para la cambiante sociedad del siglo XXI: la creatividad y el trabajo en equipo. Además, lo hacen utilizando un lenguaje que los adol...

  15. El grado de implicación del profesorado de la ESO en su formación docente.

    OpenAIRE

    Zuazua-Arcarazo, Sandra

    2013-01-01

    Mediante el siguiente trabajo se presenta la necesidad de un cambio en el sistema educativo y el papel que desempeñan los profesores en el mismo y por tanto la formación necesaria que tienen o deberían tener para hacer frente a la situacion social actual. Para esto primeramente se ha analizado el modelo educativo de varios autores referentes en el sistema educativo, como Ken Robinson, John Taylor Gatto y María Montessori y se han enfrentado dichas teorías con nuestro sistema educativo actu...

  16. Programa de desarrollo de la creatividad para alumnos de 3º de ESO en la asignatura de Física y Química

    OpenAIRE

    Alonso-Fernández, Amaya

    2006-01-01

    El sistema educativo actual requiere en sus principios la educación integral del alumno, pero en su práctica habitual la enseñanza se realiza por materias estancas en las que se exige a los alumnos ver el conocimiento desde un único punto de vista, el de la materia a impartir. Además, y de forma generalizada, los alumnos son receptores de conocimiento, pasivos en su proceso educativo. Estos factores se suman a la desconexión de los alumnos del conocimiento científico. A través de este trabajo...

  17. La motivación en la Educación Física: un estudio cuantitativo con alumnado de 2º y 4º de la ESO

    OpenAIRE

    Carcelén Marín, Sandra

    2014-01-01

    Nuestra sociedad está en continuo cambio. Las tendencias y las modas también. El alumnado de las aulas de la actualidad tienen motivaciones muy heterogéneas y diferentes a las de hace escasamente veinte años. El siguiente estudio trata de comprobar cuál es el grado de motivación de los estudiantes de Secundaria en el área de Educación Física y cómo influyen algunas variables del proceso de enseñanza-aprendizaje en dicha motivación. A través de la realización de unos cuestion...

  18. Propuestas de ense??anza de la formulaci??n qu??mica en manuales de 3?? y 4?? de ESO

    OpenAIRE

    Linares Casado, Almudena

    2013-01-01

    La crisis de la ense??anza de las ciencias es un acontecimiento muy generalizado a nivel mundial y viene caracterizado por una huida de los alumnos a campos diferentes cuando tienen que elegir sus estudios. La investigaci??n did??ctica apunta como causa principal una ense??anza inadecuada, donde el nivel de los contenidos es muy te??rico y la metodolog??a empleada, de corte tradicional. La qu??mica no se escapa a este diagn??stico. El presente trabajo estudia un t??pico muy caracter??st...

  19. Abundant Molecular Gas and Inefficient Star Formation in Intracluster Regions: Ram Pressure Stripped Tail of the Norma Galaxy Eso137-001*

    Czech Academy of Sciences Publication Activity Database

    Jáchym, Pavel; Combes, F.; Cortese, L.; Sun, M.; Kenney, J.D.P.

    2014-01-01

    Roč. 792, č. 1 (2014), 11/1-11/15 ISSN 0004-637X R&D Projects: GA ČR GAP209/12/1795 Grant - others:Rada Programu interní podpory projektů mezinárodní spolupráce AV ČR(CZ) M100031203 Program:M Institutional support: RVO:67985815 Keywords : galaxies * clusters * individual (A3627) Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 5.993, year: 2014

  20. Materiales ferromagnéticos: “¿Cómo es eso que el vector H se da vuelta?”

    OpenAIRE

    González,M. G.; Perez,L. I.; Santiago,G. D.; Veiras,F. E.

    2017-01-01

    Una de las dificultades más importantes con las que se encuentran los estudiantes de las asignaturas básicas de Electricidad y Magnetismo es la comprensión de las propiedades magnéticas de la materia. En particular, la determinación de las direcciones y sentidos de los vectores magnéticos en dispositivos simples construidos con materiales ferromagnéticos como los imanes, transformadores y electroimanes. La confusión se agudiza por la mala interpretación de las curvas B-H y/ó M-H. Los libros d...

  1. Growing Black Holes: Accretion in a Cosmological Context Proceedings of the MPA/ESO/MPE/USM Joint Astronomy Conference Held at Garching, Germany, 21-25 June 2004

    CERN Document Server

    Merloni, Andrea; Sunyaev, Rashid A

    2005-01-01

    Supermassive black holes are now believed to play an important role in the evolution of the Universe. Every respectable galaxy hosts in its center a black hole that appears to regulate the growth of the galaxy itself. In this book, leading experts in the field review the most recent theoretical and observational results on the following topics: * formation and growth of the first black holes in the Universe and their role in the formation and evolution of galaxies * the physics of black-hole accretion and the production of relativistic jets * binary black-hole mergers and gravitational radiation. Theoretical work is supplemented by the most recent exciting results from space and ground based observatories. This volume is useful research and reference tool for the entire astrophysical community.

  2. [TDAH y Matemáticas: propuestas para mejorar el proceso enseñanza – aprendizaje de los alumnos de la ESO

    OpenAIRE

    Obrer-Marco, Creu

    2014-01-01

    El trastorno por déficit de atención con hiperactividad (TDAH) tiene su origen en factores de tipo genético, neurológico y ambiental. Entre los factores de tipo neurológico, encontramos deficiencias en procesos cognitivos como la intención, la falta de inhibición de la respuesta, y los déficits en la memoria de trabajo. Estas deficiencias llevan aparejadas, en muchos casos, problemas en el aprendizaje de los alumnos que las sufren, y en particular, en el campo de las Matemática...

  3. Distributed ESO based cooperative tracking control for high-order nonlinear multiagent systems with lumped disturbance and application in multi flight simulators systems.

    Science.gov (United States)

    Cong, Zhang

    2018-03-01

    Based on extended state observer, a novel and practical design method is developed to solve the distributed cooperative tracking problem of higher-order nonlinear multiagent systems with lumped disturbance in a fixed communication topology directed graph. The proposed method is designed to guarantee all the follower nodes ultimately and uniformly converge to the leader node with bounded residual errors. The leader node, modeled as a higher-order non-autonomous nonlinear system, acts as a command generator giving commands only to a small portion of the networked follower nodes. Extended state observer is used to estimate the local states and lumped disturbance of each follower node. Moreover, each distributed controller can work independently only requiring the relative states and/or the estimated relative states information between itself and its neighbors. Finally an engineering application of multi flight simulators systems is demonstrated to test and verify the effectiveness of the proposed algorithm. Copyright © 2018 ISA. Published by Elsevier Ltd. All rights reserved.

  4. El trabajo por proyectos como eje de la programación didáctica en 4º de ESO

    OpenAIRE

    Abello Verano, Ana

    2014-01-01

    El trabajo por proyectos puede constituirse como una alternativa en la planificación del proceso de enseñanza, respondiendo a una propuesta metodológica que parece garantizar la progresión del aprendizaje pero que se ha concebido en el ámbito escolar que nos interesa como algo esporádico. La propuesta de innovación que se plantea surge con la pretensión de demostrar la viabilidad del trabajo por proyectos en el ámbito de Secundaria, proponiendo un proyecto distinto en cada trimestre y vin...

  5. ANÁLISIS DE LA PROBLEMÁTICA QUE PRESENTA EL COLECTIVO CHINO EN EL AULA DE CIENCIAS EN LA ESO Y PROPUESTA DE ADAPTACIÓN

    OpenAIRE

    Rodríguez-Martínez, Sandra

    2014-01-01

    Desde el año 2001, la llegada de inmigrantes a España se ha visto incrementada notablemente, especialmente durante los años de crecimiento económico que ha experimentado el país. En la mayoría de los casos esta venida conlleva el establecimiento de toda la familia en un nuevo lugar de residencia. En el caso de los más pequeños de la familia, el acceso al sistema educativo español se realiza desde el primer momento, a pesar de no contar con la competencia lingüística necesaria. Esto es l...

  6. La demostración en las matemáticas. Un ejemplo de aplicación en el aula con alumnos de 3.º ESO

    Directory of Open Access Journals (Sweden)

    Enrique SÁNCHEZ FREIRE

    2015-05-01

    Full Text Available En este artículo mostramos la influencia que han tenido el rigor y sus respectivos procedimientos en diferentes momentos de la Historia de las Matemáticas y como algunas demostraciones y problemas han jugado un papel importantísimo en el desarrollo de las Matemáticas. Complementaremos esta fundamentación teórica con los resultados de una experiencia de aula en donde comprobaremos que un grupo en donde se aplicó una metodología en la cual los procesos de argumentación y demostración tuvieron una especial relevancia obtuvo mejores resultados en unas pruebas de resolución de problemas que otro grupo del mismo nivel al que se le aplicó una metodología más tradicional.

  7. El diseño de contextos educativos mediados por ordenador y el aprendizaje de contenidos procedimentales de matemáticas en la ESO

    OpenAIRE

    Pifarré Turmo, Manoli; Sanuy Burgués, Jaume

    2000-01-01

    En este artículo se justifica teóricamente y se evalúa el diseño de un contexto educativo mediado por ordenador para favorecer el aprendizaje de procedimientos de resolución de problemas sobre el contenido matemático de la proporcionalidad. Los principios educativos que han guiado el diseño y el proceso de evaluación son los siguientes: a) estudiar las características del programa informático que pueden potenciar el aprendizaje de estrategias de resolución de problemas. Nuestro trabajo ...

  8. Dificultades en la evaluación de pruebas sobre determinismo biológico en la ESO y la universidad

    OpenAIRE

    Puig Mauriz, Blanca

    2009-01-01

    Este trabajo muestra los resultados de una investigación sobre el uso de pruebas, relacionadas con un enunciado científico determinista: las declaraciones de James Watson sobre las diferencias genéticas de inteligencia entre blancos y negros. La cuestión analizada es: qué tipo de datos consideran los alumnos pruebas adecuadas a favor o en contra de las afirmaciones de Watson? En concreto, se estudia si existen diferencias o no en la evaluación de pruebas relacionadas con el determinismo en di...

  9. El impacto de la escritura en dispositivos electrónicos sobre la ortografía de alumnos de ESO y Bachillerato

    OpenAIRE

    Vera-Baltanas, Francisco

    2013-01-01

    En este trabajo hemos investigado la influencia que la escritura en medios electrónicos ejerce sobre el aprendizaje y fijación de la ortografía en los alumnos de Secundaria y en la aparición de errores ortográficos en sus escritos; cuáles son los principales fenómenos que se producen y por qué están motivados, recopilando las posturas que ante estos fenómenos se mantienen. Después hemos aplicado lo estudiado a un instituto de Educación Secundaria Obligatoria concreto, deduciend...

  10. La dislexia en la clase de Primera Lengua Extranjera (Inglés): Propuesta de intervención para 1º de ESO.

    OpenAIRE

    Roa Gutiérrez, Sara

    2017-01-01

    Aproximadamente, el 10% de la población mundial presenta la dificultad conocida como dislexia. Es preciso señalar que quienes la padecen no presentan deficiencias intelectuales, ni sensoriales, aunque esta condición les supone una serie de problemas puesto que, en la sociedad actual, se le atribuye una gran importancia a la escritura y a la ortografía, siendo estas las principales áreas afectadas. Este hecho, consecuentemente, crea una situación de desigualdad entre las persona...

  11. The possible astronomical function of the El Molle stone circle at the ESO Observatory La Silla. II: The updated measurement campaign

    Science.gov (United States)

    Bernardi, Gabriella; Vecchiato, Alberto; Bucciarelli, Beatrice

    2014-07-01

    This paper reviews and updates the accounts of a previous article discussing the possible astronomical significance of a peculiar, man-made circular stone structure, located close to the European Southern Observatory in La Silla, Chile, and attributed to the El Molle culture. Thanks to further, higher-accuracy measurements in situ, we can confirm some of the original hypotheses and dismiss others, upholding the main tenets of the original work.

  12. Energy Savings Opportunity Survey (ESOS) of Schofield Barracks Family Housing, Areas A, D, E, F, I, J, K-1. Volume 1

    Science.gov (United States)

    1992-09-09

    3-(*{> SPECIFICATION GUIDE EASTROCK TECHNOLOGY, INC. The Leading Edge In Lighting Technology OPTIMUM PERFORMANCE "ER" ADAPTERS* Reduce energy...DISTRIBUTED BY The Leading Edge in Lighting Technology m ( i EASTROCK TECHNOLOGY. INC 1966 . EASTROCK TECHNOLOGY, INC. 30-40 Northfield Ave

  13. L'activitat matemàtica a l'aula virtual i presencial. Estudi comparatiu a 1r cicle d'ESO

    OpenAIRE

    Mora Cañellas, Lluís

    2012-01-01

    [cat] El problema de la investigació que es planteja aquesta tesi, té a veure amb la pregunta de si un material informàtic utilitzat de manera eficient i dissenyat adequadament pot permetre als estudiants de primer de secundària un aprenentatge significatiu de les matemàtiques en aquest nivell. Pel seu desenvolupament s'han tingut en compte dos aspectes importants: 1) el disseny de les activitats matemàtiques que hauran de realitzar els estudiants. Entenem que el treball matemàtic s'ha de des...

  14. De eso no se habla. Claves para re-pensar las relaciones entre comunicación y política

    Directory of Open Access Journals (Sweden)

    Jorge Iván Bonilla

    2007-07-01

    Full Text Available Este artículo propone una aproximación a las relaciones entre la comunicación y la política a partir de un doble desplazamiento. Por una parte, plantea la necesidad de ampliar estas relaciones más allá de los límites del campo político electoral y de las

  15. RELACION ENTRE ACTIVIDAD FÍSICA Y CONSUMO DE ALCOHOL, TABACO Y OTRAS SUSTANCIAS PERJUDICIALES PARA LA SALUD EN ALUMNOS DE ESO DEL MUNICIPIO DE AVILES

    Directory of Open Access Journals (Sweden)

    J. Rodríguez Ordax

    2010-09-01

    Full Text Available

     

    RESUMEN

    En el presente estudio nos propusimos analizar los hábitos de consumo de tabaco, alcohol y otras sustancias nocivas para la salud así como su relación con la actividad física realizada y los hábitos de práctica deportiva en una muestra representativa del alumnado de Educación Secundaria Obligatoria en el municipio de Avilés. Tanto el consumo de tabaco como el de alcohol se inician a partir de los 13 años. El porcentaje de fumadores esporádicos o frecuentes es considerablemente mayor en las chicas y no se dan diferencias de género apreciables en el consumo de alcohol. El consumo de cannabis se manifiesta claramente a los 14 años, y a los 15 ya ha sido probado en alguna ocasión por un tercio de los sujetos. Aparte del cannabis, las pastillas estimulantes son las sustancias ilegales más frecuentemente utilizadas. Al clasificar a los sujetos por su promedio diario de actividad física, expresando el consumo energético en kilocalorías por kilo de peso y día, aunque se observa que sólo un 4% se incluye en la categoría de muy inactivos, el 51% del conjunto queda fuera de lo que se consideran sujetos activos. Las medias de actividad física de los grupos de mayor consumo de tabaco y alcohol y cannabis son superiores a las de los grupos de menor consumo. Existe una tendencia a la disminución del consumo de tabaco y alcohol con el incremento en el tiempo de dedicación a la práctica deportiva.
    PALABRAS CLAVE: actividad física, deporte, tabaco, alcohol, drogas

     

    ABSTRACT

    This study was designed to analyze the consumption of tobacco, alcohol and other health-harmful substances, as well as its relationship to daily physical activity and sport practice habits in a representative sample of secondary education students from Avilés. Both tobacco and alcohol consumption start at 13 years. Percentage of sporadic or frequent smokers is clearly higher among girls and there are no gender differences in alcohol consunmption. Cannabis consupmtion develops at 14 years and a third of subjects have consumed by 15 years. The next more frequently used illegal substances are stimulating pills. When individuals are classified attending to their daily physical activity, expressed in kilocalories per kilogram and day, although only a 4% are considered as very inactive, 51% of the total are out of the active categories. Mean physical activity of groups with a high consumption of tobacco, alcohol or cannabis is more elevated than those from subjects with a low consumption. There is a tendency to the decrease of tobacco and alcohol consumption when time dedicated to sport practice increases.
    KEY WORDS: physical activity, tobacco, alcohol, drugs

  16. Taller extraescolar de tecnología (E.S.O.) para alumnos de altas capacidades. Desarrollo emocional y del talento, habilidades sociales, trabajo colaborativo y creatividad.

    OpenAIRE

    Sarmiento Zapatero, Ana

    2014-01-01

    Con este trabajo pretendemos dar a conocer las necesidades de los alumnos de altas capacidades, así como las de sus familias, quienes buscan soluciones en el sistema educativo que cubran las demandas de sus hijos. En este caso, se propone satisfacer esas necesidades educativas y personales en la etapa de educación secundaria en forma de taller extracurricular, concretamente desde el área de la tecnología y la informática. En primer lugar, hacemos una breve revisión de autores y...

  17. La physique à l'honneur Le CERN, l'ESA et l'ESO mettent la physique en scène

    CERN Multimedia

    CERN Press Office. Geneva

    2000-01-01

    Can you imagine how much physics is in a simple match of ping-pong, in throwing a boomerang, or in a musical concert? Physics is all around us and governs our lives. The World-Wide Web and mobile communication are only two examples of technologies that have rapidly found their way from science into the everyday life. But who is going to maintain these technologies and develop new ones in the future?

  18. Análisis de distintas propuestas metodológicas basadas en la creatividad aplicables a las asignaturas de Ciencias Naturales de ESO y Bachillerato

    OpenAIRE

    Escudero-Melendo, Samuel

    2013-01-01

    El sistema educativo actual, heredado de la Revolución Industrial del siglo XIX, resulta insuficiente para formar adecuadamente a los niños y adolescentes de una sociedad en continuo cambio y con un futuro que no puede intuirse. Por este motivo es necesario que los alumnos de hoy reciban una formación abierta a los cambios, que les convierta en personas capaces de adaptarse a las nuevas circunstancias, innovar y aportar valor añadido a sus acciones. Trabajar la creatividad de forma transversa...

  19. APRENDER A APRENDER: SU IMPLANTACIÓN EN EL ÁREA DE TECNOLOGÍA DE LA E.S.O.

    OpenAIRE

    Almarcha-Moreno, Santos

    2012-01-01

    Las Tecnologías de la Información y la Comunicación (TIC) están provocando en la sociedad actual cambios con una curva exponencial, lo que ahora es válido, mañana se cuestiona y pasado mañana deja de tener validez, estamos asistiendo a un auténtico cambio de época, donde la formación continua es el pilar de todo profesional, se hace necesario pues que aprendamos a aprender y así lo entienden también las autoridades educativas incorporando en la legislación educativa la competencia siete para ...

  20. ¿Significa eso que abrogamos la Torá por la confianza? Ro 3.28-31 y la biblia en lengua justa

    Directory of Open Access Journals (Sweden)

    Marlene Crüsemann

    2017-05-01

    Full Text Available M. Crüsemann explica los problemas de la traducción de Ro 3.28 en la Bíblia en lengua justa, choris ergon nomou (sin obras de la ley. Este versículo ha tenido un fuerte impacto en la tradición de la Reforma, ya que a menudo la Torá judía es pensada como de poca importancia para la cristiandad. Pero es necesario estudiar los conocimientos de Pablo respecto a la Torá en el contexto del Imperio romano. Sus estructuras poderosas enseñaron a Pablo el dominio terrorizante del pecado que impidió vivir según la Torá de Dios. Sin embargo, la gente experimenta una liberación mediante la confianza en Jesús como Mesías. Esa justificación por la fe habilita a la gente para vivir según los mandamientos de la Torá. Si la gente practica la Torá, la justicia de Dios va a florescer en el mundo. De ahí que Pablo no condena la Torá, sino que la fortalece.

  1. ESO 439-162/163 - a common proper motion binary formed by a magnetic DQ and a DC type white dwarf

    International Nuclear Information System (INIS)

    Ruiz, M.T.; Maza, J.

    1988-01-01

    In the course of a search for faint large proper motion stars, a common proper motion pair was identified having a mu = 0.38 + or - 0.03 arcsec/yr in the direction theta = 233 deg. The stars are separated by 23 arcsec and have apparent visual magnitudes 18.77 and 19.84, respectively. Spectrophotometry of the stars established that the fainter component is a cold DC white dwarf, while the brighter one is a magnetic white dwarf with strong Swan bands of C2 shifted and broadened by an about 10 to the 8th G magnetic field. 6 references

  2. Yo, TIC, Te, ConTICgo. Autoaprendizaje en grupo de Lengua y Literatura Española. 3º E.S.O.

    OpenAIRE

    Larrazábal-Inchausti, Nekane

    2012-01-01

    El sistema educativo mayoritario en los países industrializados está obsoleto. Ese es el argumento principal de los encuentros internacionales con expertos en esta materia. Todos ellos inciden en que el modelo social ha evolucionado desde una sociedad industrial hacia una tecnológica dejando atrás el modelo educativo. Esta des-conexión entre preparación de los niños y necesidad del mercado se acentúa cada día más con la implantación de las Tecnologías de la Información y la Comunicac...

  3. INFLUENCIA DE LA MOTRICIDAD FINA EN LA ES-CRITURA DE ALUMNOS CON DIFICULTADES DE APRENDIZAJE DE LA E.S.O.

    OpenAIRE

    Salgado-García, María Isabel

    2013-01-01

    La escritura es una actividad compleja que implica un entrenamiento previo para su adecuada consecución. En ocasiones, ese trabajo o entrenamiento previo no ha sido eficaz y se adquiere la escritura, pero sin un desarrollo adecuado de la motricidad dina con la consecuencia de no haber adquirido un trazo, legibilidad y limpieza adecuada. Es por ello, que hemos planteado una investigación con un alumnado de secundaria, que pese a haber recibido educación en su infancia para desarrollar unas hab...

  4. The Hamburg/ESO R-process Enhanced Star survey (HERES). XI. The highly r-process-enhanced star CS 29497-004

    Science.gov (United States)

    Hill, V.; Christlieb, N.; Beers, T. C.; Barklem, P. S.; Kratz, K.-L.; Nordström, B.; Pfeiffer, B.; Farouqi, K.

    2017-11-01

    We report an abundance analysis for the highly r-process-enhanced (r-II) star CS 29497-004, a very metal-poor giant with solar system Teff = 5013 K and [Fe/H] = -2.85, whose nature was initially discovered in the course of the HERES project. Our analysis is based on high signal-to-noise ratio, high-resolution (R 75 000) VLT/UVES spectra and MARCS model atmospheres under the assumption of local thermodynamic equilibrium, and obtains abundance measurements for a total of 46 elements, 31 of which are neutron-capture elements. As is the case for the other 25 r-II stars currently known, the heavy-element abundance pattern of CS 29497-004 well-matches a scaled solar system second peak r-process-element abundance pattern. We confirm our previous detection of Th, and demonstrate that this star does not exhibit an "actinide boost". Uranium is also detected (log ɛ(U) = -2.20 ± 0.30), albeit with a large measurement error that hampers its use as a precision cosmo-chronometer. Combining the various elemental chronometer pairs that are available for this star, we derive a mean age of 12.2 ± 3.7 Gyr using the theoretical production ratios from published waiting-point approximation models. We further explore the high-entropy wind model (Farouqi et al. 2010, ApJ, 712, 1359) production ratios arising from different neutron richness of the ejecta (Ye), and derive an age of 13.7 ± 4.4 Gyr for a best-fitting Ye = 0.447. The U/Th nuclei-chronometer is confirmed to be the most resilient to theoretical production ratios and yields an age of 16.5 ± 6.6 Gyr. Lead (Pb) is also tentatively detected in CS 29497-004, at a level compatible with a scaled solar r-process, or with the theoretical expectations for a pure r-process in this star. Based on observations collected at the European Southern Observatory, Paranal, Chile (Proposal Number 170.D-0010).Table B.1 is only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/607/A91

  5. La estructura de todas las cosas. Física y Química 2º y 3º de ESO. 6. Carga eléctrica

    OpenAIRE

    Martínez-Torregrosa, Joaquín; Alonso Sánchez, Manuel; Carbonell Gisbert, Francisco; Carrascosa Alís, Jaime; Domenech Blanco, Josep Lluís; Domenech Pastor, Ángeles; Domínguez Blay, Álvaro; Osuna García, Luis; Verdú Carbonell, Rafaela

    1993-01-01

    La construcción y utilización del versorio, la electrización de objetos grandes (palos de escoba, por ejemplo), ..., son imprescindibles para avanzar (de un modo guíado) en la comprensión de la electrostática. Una vez demostrado que existe una estructura común a todas las cosas, se plantean nuevas preguntas: si todo está formado por partículas ¿por qué existen gases, sólidos y líquidos?. En este tema, encontraremos que existe una nueva propiedad general de todos los materiales: la carga el...

  6. Unidad didáctica con diferenciación para alumnos con altas capacidades: Trigonometría para 4º de ESO

    OpenAIRE

    Comino-Royuela, Daniel

    2017-01-01

    Unlike the large number of methodologies and the wide acceptance and normalization of educational plans and differentiated measures of attention for students with special educational needs (SEN), within these there are relatively few studies and strategies of curricular adaptation for students with high intellectual abilities in today's schools. This typology of students presents common characteristics and difficulties associated with their cognitive capacities that must be addressed withi...

  7. Evolved Stars, Masers And Polarization Submm/mm/cm QUESO Workshop 2017 (QUESO2017), Centimetre-Sub-Millimetre Q&U (and V) European Southern Observatory Workshop, held 25-27 October, 2017 at ESO, Garching bei München, Germany. Online at https://www.eso.org/sci/meetings/2017/QUESO2017.html, id.35

    Science.gov (United States)

    Humphreys, Elizabeth

    2017-11-01

    Cool evolved stars on the Asymptotic Giant Branch (AGB) and Red Supergiants (RSG) often host strong masers, for example from SiO, water and OH. The maser emission can display high degrees of circular and linear polarization, potentially revealing information on magnetic field strength and morphology at different radii in the circumstellar envelopes. In this review, I will describe maser polarization theory and discuss was has been learnt so far from maser observations. I will also discuss dust polarization at (sub)mm wavelengths and the role that full polarization observations using ALMA is going to play in better characterizing evolved stars. Finally, I will talk about the potential impact of magnetic fields in the evolution of the stars, for example the shaping of AGB stars to often highly axisymmetric/aspherical Planetary Nebulae.queso2017queso2017

  8. Modified viscosity in accretion disks. Application to Galactic black hole binaries, intermediate mass black holes, and active galactic nuclei

    Science.gov (United States)

    Grzędzielski, Mikołaj; Janiuk, Agnieszka; Czerny, Bożena; Wu, Qingwen

    2017-07-01

    , which we confront now for the intermediate black hole in the source HLX-1. We reproduce the light curve of the HLX-1 source. We also compare the duration times of the model flares with the ages and bolometric luminosities of AGNs. Conclusions: With our modelling, we justify the modified μ-prescription for the stress tensor τrφ in the accretion flow in microquasars. The discovery of the Ultraluminous X-ray source HLX-1, claimed to be an intermediate black hole, gives further support to this result. The exact value of the μ parameter, as fitted to the observed light curves, may be treated as a proxy for the magnetic field strength in the accretion flow in particular sources, or their states.

  9. “Distracción controlada” en el aula: Uso de Miniquest en la enseñanza de matemáticas a alumnos con TDAH en primer ciclo de la ESO

    OpenAIRE

    Alcalde-Tejerina, María Isabel

    2012-01-01

    En la actualidad existe una gran multitud de recursos para tratar la neurodiversidad en la enseñanza de las matemáticas en secundaria. Ha sido estudiado y probado que el uso de tecnologías puede mejorar la adaptación y desarrollo cognitivo de individuos que presentan trastorno por déficit de atención con hiperactividad (TDAH). Este trabajo se centra en el uso de las Miniquest como herramientas habituales en el aula, cuya función será aumentar la concentración, motivación y el rendimiento en a...

  10. Las letras de canciones de pop-rock español como textos poéticos: Un modelo alternativo de educación literaria para e.s.o. y Bachillerato

    OpenAIRE

    Gomez Capuz, Juan

    2009-01-01

    En este trabajo intentaremos diseñar un amplio modelo de análisis de textos poéticos, aplicado a las letras de canciones de pop-rock en español. Este modelo alternativo de educación literaria está especialmente dirigido a jóvenes entre 14 y 18 años, con el objeto de que sean capaces de reconocer cierto grado de elaboración poética en las canciones pop de la vida diaria. Los aspectos poéticos estudiados en este artículo, siempre aplicados a letras de pop-rock cantado en español,...

  11. Pobre de mí en esos años oscuros / Tengo el alma amordazada detrás de los ojos / Cadáveres que son tus manos

    OpenAIRE

    Cadena Pardo, Paola

    2016-01-01

    cuando la palabra amor era pronunciable El silencio se hizo nido en el borde de mis labios y los pájaros llegaron todos a morir allí Las uñas carcomidas como una espera impasible que siempre terminó en desdicha en angustia de tiempos huérfanos en hambre que viene como el abandono sigilosa a hurtadillas danza inservible de llantos estirados hasta la punta más lejana del vacío humano hasta la soledad más sola que ni siquiera se tiene a sí misma.

  12. TO COMPARE THE SAFETY AND EFFICACY OF THREE DIFFERENT, PROTON PUMP INHIBITORS OMEPRAZOLE, ESO M EPRAZOLE AND RABEPRAZOLE IN A TRIPLE DRUG REGIMEN IN PATIENTS WITH PEPTIC ULCER DISEASE IN THE ERADICATION OF H. PYLORI INFECTION

    Directory of Open Access Journals (Sweden)

    Margaret Viola

    2015-03-01

    Full Text Available Peptic ulcer disease continues to be issue especially due to its high prevalence in the developing world. Helicobacter pylori ( H. pylori infection associated duodenal ulcers should undergo eradication therapy. There are many regimens offered for H. pylori eradication which include triple , quadruple , or sequential therapy regimens. In our study we planned to see whether these differences in pharmacokinetic properties show any difference in t he efficacy and safety parameters between treatment with omeprazole rabeprazole and esomeprazole in the triple drug regimen for eradication of H.pylori infection in peptic ulcer patients in our hospital Osmania General Hospital / Osmania Medical College , Hyderabad. MATERIALS AND METHODS: A total number of 45 patients were enrolled in the study. Patients with either sex suffering from peptic ulcer defined as ulcer crater of >2.5mm in size by endoscopy. Study Design : It was a randomized double blind , paralle l and comparative study. CONCLUSION: Two weeks after triple drug treatment , H.pylori was negative in 66.7% , 73% and 80% and Rapid urease test was negative in 53% , 60% and 66% in group A , B and C respectively. Endoscopy findings showed significant reduction in size and healing of ulcers in group A , B and C. There was improvement in signs and symptoms by 53 to 80% , after 2 weeks. Hence after therapy with triple drug regimen H.pylori eradication was 66 - 80% and healing of ulcers was 83 – 100% which was higher in Rabeprazole group. At 6 weeks , there was complete relief of signs and symptoms. At the follow up of 10 weeks there was no ulcer recurrence. No adverse effects were noted in all the groups. In conclusion , Triple drug regimen had shown to eradicate H.pylori infection in the treatment of Peptic ulcer. There was healing of ulcers in all the groups which was highly significant. There was no recurrence of peptic ulcer with these regimens in all the groups. However Rabeprazole group patients became asymptomatic ra pidly than other groups with better H.pylori eradication.

  13. Efecto de dos estilos de enseñanza (cognoscitivo versus tradicional sobre el aprendizaje de conceptos de anatomía muscular en alumnos de Educación Física de 1º de ESO

    Directory of Open Access Journals (Sweden)

    José Joaquín Muros Molina

    2010-06-01

    Full Text Available Introducción: No existen, en principio, unos estilos de enseñanza mejores que otros, pero sí puede que unos procuren que los conocimientos adquiridos perduren más en el tiempo. Método: Este estudio pretende comprobar la idoneidad de la adquisición conceptual de la nomenclatura, ubicación y aplicación de los músculos del cuerpo humano, utilizando dos estilos de enseñanza durante las clases prácticas de Educación Física (uno Descubrimiento Guiado y otro Asignación de Tareas además de compararlos con un tercer grupo de teoría sin práctica y otro grupo control. Tras ocho sesiones de tratamiento –o ausencia del mismo–, se comprobó su efecto, así como 3 meses después el posible grado de retención del aprendizaje, en una muestra total de 64 alumnos de primero de Educación Secundaria Obligatoria. Resultados: Se observó una mejora en el pos-test en los grupos en los que se aprendió la teoría mediante la práctica independientemente del estilo de enseñanza. En el grupo en que se utilizó el estilo de enseñanza cognoscitivo se mantuvo mejor, dicho aprendizaje, meses después en el retest, mientras que dicho aprendizaje descendió de forma significativa en la nomenclatura y ubicación muscular en el grupo de estilo tradicional. Conclusión: El programa de intervención utilizado fue efectivo, dándose además una mayor mejora a nivel teórico en los grupos en los que se aprendió mediante la práctica respecto al grupo sólo teórico. Además, tras tres meses, el grupo que utilizó el estilo de enseñanza Cognoscitivo logró un mayor mantenimiento de los conceptos aprendidos.

  14. Humanização do cuidado de enfermagem: o que é isso? Humanización de la atención de enfermería: qué es eso? Humanization of nursing care: what is this?

    Directory of Open Access Journals (Sweden)

    Nilza Maria de Souza Corbani

    2009-06-01

    Full Text Available Este estudo objetivou conhecer o significado que profissionais da enfermagem atribuem ao termo "humanização" e verificar como o empregam no desenvolvimento de suas atividades. A pesquisa é de natureza qualitativa, realizada por meio do método da história oral temática. Fizeram parte do estudo sete profissionais de enfermagem de um hospital de grande porte em São Paulo. Pela análise e compreensão dos significados nos discursos foi possível identificar que a expressão "cuidado humanizado" tem sido uma tradução para desumanização, tanto do profissional da enfermagem para com o cliente, como da instituição para com o profissional. Identificou-se também que esse termo tem sido usado sem a compreensão plena de seu significado.El estudio tiene por objeto intentar saber cómo los profesionales de enfermería comprenden la expresión "cuidado humanizado". La investigación es de naturaleza cualitativa, realizada por el método de historia oral temática. Los sujetos de este estudio fueron siete profesionales de enfermería de um hospitalde gran embergadura en Sao Paulo. A través del análisis y comprensión de los significados en los relatos, fue posible identificar que la expresión "cuidado humanizado" ha sido una traducción para deshumanización, tanto del profesional de enfermería para con el paciente, como de la Institución para con el profesional. Se identifica también que ese término ha sido usado sin la comprensión plena de su significado.The study was conducted with the purpose of identifying how nursing professionals understand the expression 'humanized care'. This is a qualitative research, accomplished according to the historic oral thematic method. Seven nurses of a major São Paulo hospital were chosen to partake on this study. According to the analysis and understanding of the meanings expressed on the different speeches it was possible to identify that the expression 'humanized care' has been used with the intension of expressing dehumanization, associated both with nursing professionals dehumanization, from the patient's perspective, and with dehumanization associated with the institution from the nursing professionals point of view. It was identified that this term has been used without the full understanding of its meaning.

  15. La Educación emocional como un factor de protección de las adicciones conductuales en el alumnado de 2º de E.S.O.

    OpenAIRE

    Laucirica-Basaguren, Ainhoa

    2017-01-01

    Behavioral addictions including Internet, social networks, online games and betting are still difficult to difference between normal and pathological behaviors. We could say that adolescence is a critical period for the development of different addictions; this is because the brain has not yet completed its maturation process, they tend to seek new sensations, strong emotions and they have a deeper knowledge about the new technologies. The main goal of the present work is to...

  16. ¿Qué es eso del género en arquitectura y ciudad? Todo lo que siempre quiso saber sobre género y no se atrevió a preguntar a arquitectos y urbanistas

    OpenAIRE

    Hernández Pezzi, Carlos

    2016-01-01

    Se presenta un análisis de las raíces de la visión de género sobre arquitectura y ciudad, partiendo de los grandes mitos arquitectónicos del siglo XX y de los impactos en la ciudad de las teorías del Movimiento Moderno y especialmente Le Corbusier. Zaha Hadid y Rem Koolhaas, se revisan como teóricos y grandes arquitectos del siglo XXI. Son 90 pantallas de planos, fotos, gráficos y cómics que pretenden ilustrar los desequilibrios de género y las contradicciones de la ciudad contemp...

  17. La gramática en la enseñanza del español como lengua extranjera: ¡Eso está hecho! Aprendiendo el participio

    Directory of Open Access Journals (Sweden)

    Reyes Llopis García

    2005-01-01

    Full Text Available Podemos definir la palabra Gramática desde dos perspectivas diferentes, aunque relacionadas entre sí: la primera se refiere al saber implícito que cada hablante de una lengua tiene de ésta, y cómo desde su conocimiento interno es capaz de comunicarse de forma competente y comprensible. La segunda visión de gramática es aquélla que se centra en explicar este conocimiento mediante normas, reglas y descripciones que funcionan como guía para el correcto y eficaz uso de la lengua misma.

  18. La gramática en la enseñanza del español como lengua extranjera: ¡Eso está hecho! Aprendiendo el participio

    Directory of Open Access Journals (Sweden)

    Reyes Llopis García

    2012-08-01

    Full Text Available Podemos definir la palabra Gramática desde dos perspectivas diferentes, aunque relacionadas entre sí: la primera se refiere al saber implícito que cada hablante de una lengua tiene de ésta, y cómo desde su conocimiento interno es capaz de comunicarse de forma competente y comprensible. La segunda visión de gramática es aquélla que se centra en explicar este conocimiento mediante normas, reglas y descripciones que funcionan como guía para el correcto y eficaz uso de la lengua misma.

  19. La exención (in)visible de la Educación Física en la E.S.O. y el Bachillerato de principios del siglo XXI

    OpenAIRE

    Rodríguez de la Cruz, Julio César

    2015-01-01

    Esta tesis se adentra en el estudio del trabajo que los profesores de Educación Física en Secundaria y en Bachillerato desempeñan con los alumnos con dificultades de aprendizaje asociadas a discapacidad motriz. En concreto, la investigación nos aproxima al concepto y uso de la exención, tanto en el saber teórico como en la práctica, en la Educación Física de finales del siglo XX y primera década del XXI. A lo largo de la tesis se pondrá de manifiesto que la exención es una realidad que aún pe...

  20. “[…] Y por eso fue que llegué a la ciudad”. Movilidad social, conflicto armado y territorio en el Pacífico Colombiano. Voces de estudiantes afrocolombianos de la FUAC

    Directory of Open Access Journals (Sweden)

    Omar Jerónimo Prieto Ruiz

    2016-12-01

    Full Text Available El conflicto armado que por décadas ha azotado al país tiene en la región Pacífica uno de sus escenarios de reproducción. La región, históricamente olvidada y marginalizada tanto del proceso de constitución de la Nación, como de las obligaciones estatales en materia de Derechos Humanos, padece distintas formas de violencia. Muchos estudiantes de la Universidad Autónoma llegan a Bogotá provenientes de alguno de los cuatro Departamentos que componen el litoral, en busca de mejores oportunidades de vida. Distintas son las razones que explican el viaje. El texto pretende hacer visible esa movilidad con base en los testimonios de estudiantes afrocolombianos.

  1. Análisis del desarrollo de las competencias básicas en el currículum de la Educación Física en la ESO en la Marina Baixa. Un estudio de caso

    Directory of Open Access Journals (Sweden)

    Julio Barrachina Peris

    2012-12-01

    Full Text Available Con la aprobación de la Ley Orgánica de Educación (LOE, 2006 las competencias básicas cobran un especial protagonismo en la enseñanza. Existe poco conocimiento sobre su desarrollo práctico en el currículum de la educación física en secundaria. El presente artículo resume una investigación realizada en el curso 2010 en los IES de la comarca de La Marina Baixa (Alicante. Enmarcada en la metodología cualitativa, se presenta un estudio de caso sobre una muestra de 28 profesores, con el objetivo de averiguar qué percepción tienen de éstas, qué estrategias emplean para integrarlas en la programación y cuáles son los principales obstáculos con que se encuentran para materializarlas en la práctica. Los datos se analizaron con el programa Aquad 6 y los resultados evidencian que el enfoque por competencias aún está consolidándose entre el profesorado. Las principales estrategias empleadas para su desarrollo se relacionan con la toma de decisiones en el diseño curricular, seguidas por la promoción de la participación y motivación del alumnado. En cuanto a los obstáculos, destacan la escasez y precariedad de los recursos disponibles, la falta de información y formación recibida por el profesorado, así como la escasa implicación de profesores, padres y alumnos.

  2. Atbildība pret trešajām personām būvniecībā un par ekspluatācijā esošu būvi

    OpenAIRE

    Zalamane, Adele

    2015-01-01

    Maģistra darba tēma ir civiltiesiskā atbildība pret trešajām personām par aizskārumu, kura pamatā ir būvniecības laikā pieļautas kļūdas. Darba mērķis ir noskaidrot pastāvošo regulējumu, apzināt tā trūkumus un piedāvāt risinājumus. Lai izpētītu civiltiesiskās atbildības regulējumu pret trešajām personām, ievērojot nesenos normatīvo aktu grozījumus būvniecības nozarē, izpētīts atbildības regulējums Būvniecības likumā, kurš bija spēkā līdz 2014.gada 1.oktobrim, un jaunajā Būvniecības likumā. ...

  3. News and Views: Life on Mars? Astronomical model is world's biggest; Prizes for identifying dark matter; NAM 2013: call for sessions; Paintballing to save the planet; Happy Birthday ESO; Dark sky park grows

    Science.gov (United States)

    2012-12-01

    The University of Edinburgh, crowdsourcing website Kaggle and Winton Capital Management have joined forces to launch a competition to identify dark matter haloes. The Scientific Organizing Committee of the RAS National Astronomy Meeting 2013, the UK Solar Physics and Magnetosphere, Ionosphere and Solar-Terrestrial meetings, are seeking nominations for parallel discussion session themes. A winner of the 2012 Move an Asteroid Technical Paper Competition suggests painting asteroids white in order to boost their albedo and take advantage of solar radiation pressure to alter their orbits.

  4. IDENTIFICACIÓN DEL GRADO DE LATERALIZACIÓN EN ALUMNOS DE 2º CURSO DE LA E.S.O., Y SU RELACIÓN CON EL FRACASO ESCOLAR.

    OpenAIRE

    López-Sorlózano, José María

    2013-01-01

    Son muchos los trabajos de investigación y autores que relacionan parte del fracaso escolar y alteraciones de la lateralidad (contrariada, cruzada o mal definida). En el presente trabajo se les ha practicado un test de dominancia lateral a 44 niños con edades comprendidas entre los 13 y los 15 años de segundo curso de la Educación Secundaria Obligatoria, en un Instituto de Enseñanza Secundaria Pública de la Comunidad Autónoma de Andalucía, dónde se identifica la dominancia manu...

  5. Análisis de la percepción del proceso de la Educación de la Afectividad en alumnos de Segundo Ciclo de la ESO. Estudio de caso de centro educativo de Barcelona.

    OpenAIRE

    Fernández, Elisa

    2012-01-01

    Los problemas de disrupción en el aula generan estrés en el profesorado y un clima educativo poco favorable para el aprendizaje. Muchos profesores manifiestan no tener formación para enfrentarse a estas situaciones. En este contexto, cada vez más se hace necesaria una educación integral de la persona, intelectual, afectiva y física. A pesar de marcar fuertemente al adolescente, la afectividad no ha sido valorada y tenida en cuenta lo suficiente en la educación en el pasado. ...

  6. EL USO DE LA PLATAFORMA VIRTUAL DE APRENDIZAJE “CLAROLINE” COMO EJE DE APOYO CURRICULAR PARA LA MEJORA DE LA ENSEÑANZA DE EPV EN 4º ESO.

    OpenAIRE

    Martí-Guerola, María del Pilar

    2013-01-01

    A lo largo del presente trabajo de investigación, se muestra cómo a través del uso de una plataforma virtual de aprendizaje o Sistema de Gestión de Aprendizaje (SGA) como “Claroline”, se puede crear un espacio educativo virtual adaptado a las necesidades de la asignatura de EPV. El SGA propuesto en el presente trabajo, contendrá diversos elementos didácticos destinados a ayudar a los alumnos en su proceso de aprendizaje y de alfabetización digital, sirviendo al mismo tiempo ...

  7. Efecto de dos estilos de enseñanza (cognoscitivo versus tradicional) sobre el aprendizaje de conceptos de anatomía muscular en alumnos de Educación Física de 1º de ESO

    OpenAIRE

    Muros Molina, José Joaquín; Som Castillo, Antonio; Leyva Rodríguez, Ana Isabel; Zabala Díaz, Mikel

    2010-01-01

    Introducción: No existen, en principio, unos estilos de enseñanza mejores que otros, pero sí puede que unos procuren que los conocimientos adquiridos perduren más en el tiempo. Método: Este estudio pretende comprobar la idoneidad de la adquisición conceptual de la nomenclatura, ubicación y aplicación de los músculos del cuerpo humano, utilizando dos estilos de enseñanza durante las clases prácticas de Educación Física (uno Descubrimiento Guiado y otro Asignación de Tareas) además de compararl...

  8. Importancia del aprendizaje de los principios básicos de alimentación y nutrición para los alumnos de ESO. Desarrollo de los recursos didácticos que sirvan para adquirir dichos conocimientos.

    OpenAIRE

    Casellas Ferrer, Alberto

    2014-01-01

    El estilo de vida, la salud y la personalidad están muy relacionados con la educación, no obstante no se le ha dado a este hecho la importancia que se merece y se ha obviado el aspecto “educativo” de los hábitos de salud, al hablar de proceso salud-enfermedad. Actualmente se considera imprescindible la intervención educativa para prevenir la enfermedad y mejorar la salud de la población La revisión de programas de educación nutricional en el entorno escolar, demuestra que se puede prevenir...

  9. Relativistic Astrophysics Legacy and Cosmology – Einstein’s Proceedings of the MPE/USM/MPA/ESO Joint Astronomy Conference Held in Munich, Germany, 7-11 November 2005

    CERN Document Server

    Aschenbach, B; Hasinger, G; Leibundgut, B

    2007-01-01

    "Relativistic Astrophysics and Cosmology" have developed into important and fruitful research areas with a hitherto unprecented public interest. The year 2005, which marked the 100th anniversary of the 'annus mirabilis', that year in which Albert Einstein published three of his most important scientific papers, was the perfect opportunity to review and to present the current scientific understanding of relativistic topics including cosmological models, cosmology related measurements, the large scale properties of the Universe, dark matter and dark energy, the theory of gravity, black holes, theory and measurements, active galactic nuclei, clusters of galaxies, jets. Each of these subjects is addressed and the book represents a useful reference tool both for the expert and the new-comer in these fields.

  10. Una aproximación didáctica a la aplicación del constructivismo en la enseñanza de Ciencias de la Naturaleza en 3º ESO

    OpenAIRE

    Rodríguez-Gate, Sara

    2013-01-01

    En la actualidad, las empresas demandan cada vez más un perfil en el que la persona sea flexible y sepa trabajar en equipo. Dada la cantidad ingente de información a la que se tiene acceso hoy en día, es esencial dotar a los alumnos con la capacidad para gestionarla. En este marco de partida, la escuela debe responder a estas demandas formando personas que sepan adaptarse a cualquier contexto y cambio en la sociedad. En definitiva, hay que enseñar a los alumnos a aprender por ...

  11. Estudio de recursos y estrategias de aprendizaje para ser implantados en los Rincones de Actividad en la materia de Física y Química de 4º de ESO

    OpenAIRE

    Goikoetxea-Canellada, Oihana

    2014-01-01

    El presente Trabajo de Fin de Máster pretende proponer un proyecto para la materia de Física y Química. Se presenta un estudio sobre la metodología basada en Rincones de Actividad, una organización de aula que promueve la implicación y la motivación del alumnado en su aprendizaje. Una delimitación alternativa de los espacios que, mediante la experiencia colectiva, incide en el desarrollo de la responsabilidad y la autonomía del educando. Se han analizado los nuevos enfoques metodológicos que ...

  12. La motivación y la Teoría de las Inteligencias Múltiples: Prácticas Pedagógicas en Matemáticas de 3º de ESO

    OpenAIRE

    Pascual-Mauriz, María del Pilar

    2013-01-01

    La teoría de las Inteligencias Múltiples (IM) de Howard Gardner tiene hoy una gran influencia en la forma de entender la enseñanza y en la definición de los objetivos y competencias. En los centros que han decidido implantar un proyecto educativo basado en la teoría de las IM, las prácticas educativas que facilitan el desarrollo de las diferentes inteligencias han pasado de ser una metodología complementaria a ser la metodología principal. La mayor parte de los contenidos de asignaturas como ...

  13. La orientación en Educación Secundaria desde la perspectiva de los alumnos de 2º ciclo de E.S.O. y sus padres

    OpenAIRE

    Muñoz Rigores, Manuel

    2016-01-01

    La presente investigación se ha planteado como objetivo principal explorar el conocimiento y valoración que de la práctica orientadora hace el alumnado de 3º y 4º de educación secundaria y sus padres partiendo de sus percepciones, opiniones, conocimientos y otros aspectos referidos a las funciones y tareas del profesorado tutor y de los orientadores de esta etapa educativa en los centros públicos de Huelva y su provincia. Los instrumentos utilizados fueron: por una parte, un cuestionario p...

  14. VizieR Online Data Catalog: C and O abundances in stellar populations (Nissen+, 2014)

    Science.gov (United States)

    Nissen, P. E.; Chen, Y. Q.; Carigi, L.; Schuster, W. J.; Zhao, G.

    2014-06-01

    Equivalent widths of C and O lines and the abundances derived are given for 66 disk stars with ESO3.6m/HARPS and ESO2.2m/FEROS spectra and for 85 halo and thick-disk stars with ESO-VLT/UVES or NOT/FIES spectra available in the ESO Science Archive. (4 data files).

  15. The Employee as the Unknown Actor? A Discourse Analysis of the Employee Share Ownership Debate with Special Emphasis on Central and Eastern Europe

    Directory of Open Access Journals (Sweden)

    Kranz Olaf

    2016-05-01

    Full Text Available Background and purpose: Although employee share ownership (ESO deserves of a long tradition, we still know little about employees’ perspectives about ESO. The lack of knowledge about the employees’ attitudes towards ESO is discursively filled in the ESO debate. This paper challenges that deficit by carrying out a semantic analysis of the literature with the aim to identify the various actor constructions used implicitly in the ESO discourse.

  16. Soluble serum VCAM-1, whole blood mRNA expression and treatment response in natalizumab-treated multiple sclerosis

    DEFF Research Database (Denmark)

    Petersen, E R; Søndergaard, H B; Oturai, A B

    2016-01-01

    Background Natalizumab reduces disease activity in multiple sclerosis (MS). Natalizumab binds to the very late antigen-4 and inhibits vascular cell adhesion molecule-1 (VCAM-1)-mediated transmigration of immune cells across the blood-brain-barrier. This is associated with decreased serum concentr......Background Natalizumab reduces disease activity in multiple sclerosis (MS). Natalizumab binds to the very late antigen-4 and inhibits vascular cell adhesion molecule-1 (VCAM-1)-mediated transmigration of immune cells across the blood-brain-barrier. This is associated with decreased serum...... concentrations of soluble (s)VCAM-1 and an altered composition of immune cell-subsets in the blood. Objective We aimed to examine if sVCAM-1 serum concentrations and whole blood mRNA expression levels of immune activation biomarkers is associated with disease activity in natalizumab-treated MS-patients. Methods...... sVCAM-1 serum concentrations and whole blood mRNA expression were measured in blood samples from untreated RRMS-patients and from two independent groups of natalizumab-treated patients. Results sVCAM-1 serum concentrations and whole blood expression of HLX1 and IL1B mRNA were lower, whereas...

  17. Gene mapping of Usher syndrome type IIa: Localization of the gene to a 2.1-cM segment on chromosome 1q41

    Energy Technology Data Exchange (ETDEWEB)

    Kimberling, W.J.; Weston, M.D.; Ing, P.S.; Connolly, C. [Center for Hereditary Communication Disorders, Omaha, NE (United States); Sumegi, J. [Univ. of Nebraska, Omaha, NE (United States); Moeller, C. [Univ. of Linkoeping Medical School (Sweden); Aarem, A. van; Cremers, C.W.R.J. [University Hospital, Nijmegen (Netherlands); Martini, A.; Milani, M. [and others

    1995-01-01

    Usher syndrome type II is associated with hearing loss and retinitis pigmentosa but not with any vestibular problems. It is known to be genetically heterogeneous, and one locus (termed USH2A) has been linked to chromosome 1q41. In an effort to refine the localization of USH2A, the genetic map of the region between and adjacent to the marker loci previously recognized as flanking USH2A (D1S70 and PPOL) is updated. Analysis of marker data on 68 Usher II families places the USH2A gene into a 2.1-cM region between the markers D1S237 and D1S229. The gene for transforming growth factor {beta}2 (TGFB2) and the gene for the homeodomain box (HLX1) are both eliminated as candidates for USH2A, by virtue of their localization outside these flanking markers. The earlier finding of genetic heterogeneity was confirmed in six new families, and the proportion of unlinked Usher II families is estimated at 12.5%. The placement of the USH2A gene into this region will aid in the physical mapping and isolation of the gene itself. 30 refs., 4 figs., 2 tabs.

  18. Opposing roles of STAT4 and Dnmt3a in Th1 gene regulation

    Science.gov (United States)

    Pham, Duy; Yu, Qing; Walline, Crystal C.; Muthukrishnan, Rajarajeswari; Blum, Janice S.; Kaplan, Mark H.

    2013-01-01

    The Signal Transducer and Activator of Transcription factor STAT4 is a critical regulator of Th1 differentiation and inflammatory disease. Yet, how STAT4 regulates gene expression is still unclear. In this report, we define a STAT4-dependent sequence of events including H3K4 methylation, Jmjd3 association with STAT4 target loci, and a Jmjd3-dependent decrease in H3K27 trimethylation and DNA methyltransferase (Dnmt) 3a association with STAT4 target loci. Dnmt3a has an obligate role in repressing Th1 gene expression, and in Th1 cultures deficient in both STAT4 and Dnmt3a, there is recovery in the expression of a subset of Th1 genes that is sufficient to increase IFNγ production. Moreover, although STAT4-deficient mice are protected from the development of EAE, mice deficient in STAT4 and conditionally-deficient in Dnmt3a in T cells develop paralysis. Th1 genes that are de-repressed in the absence of Dnmt3a have greater induction following the ectopic expression of the Th1-associated transcription factors T-bet and Hlx1. Together, these data demonstrate that STAT4 and Dnmt3a play opposing roles in regulating Th1 gene expression, and that one mechanism for STAT4-dependent gene programming is in establishing a de-repressed genetic state susceptible to transactivation by additional fate-determining transcription factors. PMID:23772023

  19. El método Pilates en la educación física. Programa de intervención en la salud escolar: calidad de vida, estado físico y actitud postural de estudiantes de ESO en Cantabria [The Pilates Method in Physical Education. School Health Intervention Programme: Q

    Directory of Open Access Journals (Sweden)

    Rosa Blanco Martínez

    2018-01-01

    Full Text Available El presente trabajo plantea determinar los efectos de una intervención basada en el método Pilates en el alumnado de educación secundaria de Cantabria, y de manera específica: a analizar los efectos del programa de método Pilates (MP sobre la salud y calidad de vida del alumnado; b analizar dichos efectos sobre su estado físico, y c analizarlos sobre su actitud postural.La investigación tiene dos fases: 1 un estudio cuasi-experimetal piloto donde se aplica un programa de MP (unidad didáctica, UD, de Pilates de 8 sesiones a un grupo de 57 estudiantes europeos de educación secundaria, y 2 otro estudio cuasi-experimental donde se realiza una intervención escolar con un programa de MP (UD de Pilates de 6 sesiones sobre una muestra de 476 estudiantes de educación secundaria de Cantabria. En ambas fases se contó con un grupo experimental (GE y un grupo de control (GC. El programa de MP fue dirigido por el profesorado de EF, por lo que se llevó a cabo un curso previo de formación teórico-práctico de MP, específico de la UD de Pilates escolar, para el profesorado que participaba en la investigación. Como principales resultados se hallaron diferencias significativas en función del sexo, siendo las chicas las que mostraron un porcentaje de práctica de AF más bajo (p = 0.001 y existiendo diferencias también en cuanto al número de horas de práctica de AF por semana (p = 0.006. Tras el programa con MP, el GE obtuvo significativamente mejores resultados en la valoración del estado físico, en el test de fuerza abdominal (f = 6.329 y p < 0.01 que mide la fuerza-resistencia de la musculatura flexora abdominal (FFT y también se obtuvieron mejores resultados en el test de zancada frontal, que mide la flexibilidad de psoas-ilíaco, glúteo y musculatura isquiosural (FMS respecto al GC. En la valoración de la actitud postural (AP, el GE obtuvo mejores resultados en las pruebas de media flexión de piernas, elevación lateral, cien y giro hacia arriba (p < 0.01. En cuanto al GE, un 62% percibieron beneficios tras la práctica de MP siendo los más valorados: la mejora de la postura, la mejora de la flexibilidad, mayor relajación y encontrarse mejor físicamente.En conclusión, la intervención con MP comportó mejoras significativas en el estado físico (FFT y FMS de los estudiantes del GE, así como sobre la AP del GE. Estos beneficios coinciden con los percibidos por el GE tras la intervención con MP. Se encontraron diferencias significativas por cuestión de género según la variable analizada. No se encontraron mejoras significativas en la salud y en la calidad de vida tras la aplicación del MP.

  20. Uso de las TIC para motivar y facilitar el aprendizaje sobre el enlace químico correspondiente a la asignatura de Física y Química en alumnos de 4º de la E.S.O.

    OpenAIRE

    Angulo-González, Francisco Javier

    2013-01-01

    La desmotivación de nuestros alumnos hace que la educación y la enseñanza se conviertan en una dura tarea de manera continua a lo largo del curso. Los resultados académicos se ven mermados debido al poco interés que muestran los alumnos y que los docentes, con la manera de impartir las unidades didácticas de una forma tradicional, infunden en ellos. Esto hace que el abandono escolar aumente, que España se encuentre entre los últimos países europeos en cuanto a resultados aca...

  1. Robotic simulation with 2.0 tools to develop key competencies in Secondary Education. A case study Simulación robótica con herramientas 2.0 para el desarrollo de competencias básicas en ESO. Un estudio de casos

    Directory of Open Access Journals (Sweden)

    Esteban Vázquez Cano

    2012-07-01

    Full Text Available Normal.dotm 0 0 1 171 975 Universidad de Salamanca 8 1 1197 12.0 0 false 18 pt 18 pt 0 0 false false false /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} The paper presents a contextualized research in a secondary school in the province of Toledo in which he examines the design of a robotic simulation activity for the development of key competencies from an interdisciplinary and transversely way between the subjects of Technology, Spanish Language, English and Plastic and Visual Education. The design of the activity consisted on the development of an interdisciplinary project for the construction of a worm robot with an electronic device for measuring lengths. 2.0 free tools were used for designing it and  supported the development of four key competencies: Google Sketchup, Prezi, Glosgter and Windows Movie Maker. We analyzed the findings of teachers and students on the feasibility of robotic simulation through the project work as a teaching strategy and especially the improvement of indicators of the following basic skills: language, learning to learn, information processing and digital competence knowledge and interaction with the physical world. The results show a high appreciation of students and teachers about the project and its functionality in the cross and interdisciplinary development of key competencies. Normal.dotm 0 0 1 195 1112 Universidad de Salamanca 9 2 1365 12.0 0 false 18 pt 18 pt 0 0 false false false /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} El artículo presenta una investigación contextualizada en un centro de enseñanza secundaria de la provincia de Toledo en el que se analiza el diseño de una actividad de simulación robótica para el desarrollo de competencias básicas de forma transversal e interdisciplinar entre las materias de Tecnología, Lengua española, Lengua inglesa y Educación Plástica y Visual. El diseño de la actividad consistió en el desarrollo de un proyecto interdisciplinar para la construcción de un robot gusano con un dispositivo electrónico que lo convierte en un robot para medir longitudes. Para su diseño y desarrollo se utilizaron cuatro herramientas 2.0 gratuitas que sirvieron de soporte al desarrollo de competencias básicas: Google Sketchup, Prezi, Glosgter y Windows Movie Maker. Se analizaron las apreciaciones de docentes y alumnos sobre la viabilidad de la simulación robótica a través del trabajo por proyectos como estrategia didáctica y especialmente la mejora de indicadores de las siguientes competencias básicas: lingüística, aprender a aprender, tratamiento de la información y competencia digital y conocimiento y la interacción con el mundo físico. Los resultados obtenidos muestran una alta apreciación de alumnado y profesorado sobre el proyecto y su funcionalidad en el desarrollo transversal e interdisciplinar de las competencias básicas.

  2. UN AÑO Y MEDIO DE ESTATUTO DEL TRABAJO AUTÓNOMO Y SU INFRADESARROLLO: Y EN ESO LLEGÓ UNA GRAN CRISIS ECONÓMICA/ONE YEAR AND A HALF OF STATUTE OF THE AUTONOMOUS WORK AND HIS UNDERDEVELOPMENT (AND IN THAT A BIG CRISIS CAME

    Directory of Open Access Journals (Sweden)

    Frederic LÓPEZ i MORA

    2008-09-01

    Full Text Available El trabajo autónomo tiene una notable implantación en España y cumple una relativamente amplia funcionalidad en nuestro sistema económico y social. En el conjunto del tejido empresarial, es absolutamente hegemónica la figura del autónomo que no dispone de asalariados a su servicio, junto con el que emplea hasta dos trabajadores por cuenta ajena. Antes de la aprobación parlamentaria de la Ley 20/2007, su regulación resultaba muy fragmentaria, deficiente y obsoleta, sin atender a las profundas transformaciones que se han sucedido tras la llegada del posfordismo a este sector. Con la entrada en vigor de dicha norma, España dispone de una ley-marco, pionera en la Unión Europea y seguramente en el mundo, que establece un régimen jurídico mínimo, común e inderogable para todos los autónomos que producen en nuestro país. Este estudio, fundamentalmente jurídico, no se centra en el análisis de la citada disposición – bautizada como Estatuto del Trabajo Autónomo -, sino en su posterior desarrollo desde su aprobación el 11 julio del 2007 hasta finales del año 2008; sin embargo, a la vista de que ese proceso ha venido a coincidir con una profunda crisis económica, la indagación se complementa con un apunte crítico sobre las principales medidas de choque adoptadas por los poderes públicos para afrontar el impacto de la recesión sobre los trabajadores autónomos, para cerrarse con una reflexión sobre las implicaciones estructurales y desafíos que supone la vigencia dicho Estatuto tanto en España como en el seno de la Unión Europea./The autonomous work has a notable implantation in Spain and fulfills a relatively wide functionality in our economic and social system. In the set of the managerial, there is absolutely hegemonic the figure of the autonomous one who has not wage earners to his service, together with that it uses up to two workpeople for foreign account. Before the parliamentary approval of the Law 20/2007, his regulation was turning out to be very fragmentary, deficient and obsolete, without attending on the deep transformations that have happened after the arrival of the posfordism to this sector. With the coming into effect of the above mentioned norm, Spain has a law - frame, pioneer in the European Union and surely in the world, which establishes a juridical minimal pattern, common and unrepealable for all the autonomous ones that produce in our country. This study, fundamentally juridical, does not center on the analysis of the said disposition – baptized as Statute of the Autonomous Work-, but on his later development from his approval on July 12, 2007 until ends of the year 2008; but, in view of that this process has come to coincide with a deep economic crisis, the investigation complements itself with a critical note on the principal measurements of shock adopted by public power to confront the impact of the recession on the autonomous workpeople, to close with a reflection on the structural implications and challenges that supposes the above mentioned Statute, both in Spain and in the bosom of the European Union.

  3. Proyecto de intervención para mejorar las competencias emocionales y el control emocional de los adolescentes de tercero de Educación Secundaria Obligatoria (ESO) del colegio “El Centro Inglés” de Cádiz

    OpenAIRE

    Adams, Megan

    2015-01-01

    Este Trabajo de Fin de Grado (TFG) constituye una iniciación a la investigación educativa. El tema central es la regulación emocional, que es la capacidad para manejar de forma apropiada las emociones y supone tomar conciencia de la relación entre emoción, cognición y comportamiento y supone la regulación de las emociones propias y de los demás en distintos contextos y situaciones. Los objetivos generales del trabajo son elaborar un marco teórico sobre el concepto de inteligencia emocional, l...

  4. "Faz isso, faz aquilo, mas eu tô caindo...": compreendendo a Doença de Chron "Haz eso, haz aquello, mas yo estoy cayendo...": comprendiendo la enfermedad de Chron "Do this, do that, but I'm falling down...": understanding Chron's disease

    Directory of Open Access Journals (Sweden)

    Andrea Castelli

    2007-03-01

    Full Text Available Buscando compreender o significado e o impacto da doença de Chron na vida de seu portador, desenvolvemos este estudo, de natureza qualitativa, optando pela História Oral de Vida como referencial metodológico, tendo como base filosófica a corrente monista, advinda de Hipócrates, em que a prática médica tem por objeto a pessoa doente em sua totalidade, levando em conta seu temperamento e a sua história. A terapêutica deve restabelecer a harmonia da pessoa com seu ambiente e consigo mesma , trata-se de uma medicina integradora e dinâmica, que corresponde aos primórdios da medicina psicossomática. O estudo da doença de Chron, por meio da compreensão psicossomática, permitiu que analisássemos os sintomas, não como corpos estranhos a serem erradicados, mas como mensageiros de um apelo a ser compreendido. Por intermédio deles, pode ser resgatada a história de um indivíduo que, se conscientemente vivida e analisada, permite o desenvolvimento até níveis mais evoluídos de entendimento.Buscando comprender el significado y el impacto de la Enfermedad de Chron en la vida de su portador, desarrollamos este estudio, de naturaleza cualitativa, optando por la Historia Oral de Vida como referencial metodológico, y teniendo como base filosófica la corriente monista, advenida de Hipócrates, donde la práctica médica tiene por objeto a la persona enferma en su totalidad, teniendo en cuenta su temperamento y su historia. La terapéutica debe restablecer la armonía de la persona con su ambiente y consigo misma y se trata de una medicina integradora y dinámica, que corresponde a los inicios de la medicina psicosomática. El Estudio de la enfermedad de Chron, por medio de la comprensión psicosomática, permitió que analizásemos los síntomas, no como cuerpos extraños a ser erradicados, mas como mensajeros de un apelo a ser comprendido. Por intermedio de ellos, puede ser rescatada la historia de un individuo que, si conscientemente vivida y analizada, permite el desarrollo hasta niveles más evolucionados de entendimiento.This qualitative study was developed as an effort to understand the meaning and the impact of Chron's Disease on the carrier's life, opting for Life Oral History as the methodological reference and having as a base Hippocrates' monist philosophical school, for which the medical practice focuses on the sick person in his/her totality, taking into consideration his/her temperament and background. The therapeutics must reestablish the person's harmony with his/her environment and with himself/herself. It is an integrative, dynamic medicine that corresponds to the beginnings of psychosomatic medicine. The studies on Chron's Disease, through psychosomatic understanding, made possible to analyze the symptoms not as alien bodies to be eradicated, but as messengers of an appeal to be understood. Through them, the person's history can be recovered, which makes possible the development to more advanced levels of understanding if it is experienced and analyzed consciously.

  5. Plant oil-based shape memory polymer using acrylic monolith

    Directory of Open Access Journals (Sweden)

    T. Tsujimoto

    2015-09-01

    Full Text Available This article deals with the synthesis of a plant oil-based material using acrylic monolith. An acrylic monolith bearing oxirane groups was prepared via simple technique that involved the dissolution of poly(glycidyl methacrylate-comethyl methacrylate (PGMA in ethanolic – aqueous solution by heating and subsequent cooling. The PGMA monolith had topologically porous structure, which was attributed to the phase separation of the polymer solution. The PGMA monolith was impregnated by epoxidized soybean oil (ESO containing thermally-latent catalyst, and the subsequent curing produced a crosslinked material with relatively good transparency. The Young’s modulus and the tensile strength of polyESO/PGMA increased compared with the ESO homopolymer. The strain at break of polyESO/PGMA was larger than that of the ESO homopolymer and crosslinked PGMA. Furthermore, polyESO/PGMA exhibited good shape memory-recovery behavior.

  6. Percutaneous closure of patent foramen ovale in patients with cryptogenic embolism: a network meta-analysis.

    Science.gov (United States)

    Stortecky, Stefan; da Costa, Bruno R; Mattle, Heinrich P; Carroll, John; Hornung, Marius; Sievert, Horst; Trelle, Sven; Windecker, Stephan; Meier, Bernhard; Jüni, Peter

    2015-01-07

    Up to 40% of ischaemic strokes are cryptogenic. A strong association between cryptogenic stroke and the prevalence of patent foramen ovale (PFO) suggests paradoxical embolism via PFO as a potential cause. Randomized trials failed to demonstrate superiority of PFO closure over medical therapy. Randomized trials comparing percutaneous PFO closure against medical therapy or devices head-to-head published or presented by March 2013 were identified through a systematic search. We performed a network meta-analysis to determine the effectiveness and safety of PFO closure with different devices when compared with medical therapy. We included four randomized trials (2963 patients with 9309 patient-years). Investigated devices were Amplatzer (AMP), STARFlex (STF), and HELEX (HLX). Patients allocated to PFO closure with AMP were less likely to experience a stroke than patients allocated to medical therapy [rate ratio (RR) 0.39; 95% CI: 0.17-0.84]. No significant differences were found for STF (RR 1.01; 95% CI: 0.44-2.41), and HLX (RR, 0.71; 95% CI: 0.17-2.78) when compared with medical therapy. The probability to be best in preventing strokes was 77.1% for AMP, 20.9% for HLX, 1.7% for STF, and 0.4% for medical therapy. No significant differences were found for transient ischaemic attack and death. The risk of new-onset atrial fibrillation was more pronounced for STF (RR 7.67; 95% CI: 3.25-19.63), than AMP (RR 2.14; 95% CI: 1.00-4.62) and HLX (RR 1.33; 95%-CI 0.33-4.50), when compared with medical therapy. The effectiveness of PFO closure depends on the device used. PFO closure with AMP appears superior to medical therapy in preventing strokes in patients with cryptogenic embolism. Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2014. For permissions please email: journals.permissions@oup.com.

  7. The Role of Learning Strategies in Second Language Acquisition: Strategy Use by Students of English

    Science.gov (United States)

    1987-01-01

    pensando. Me estaba diciendo ahora mismo quo puslera mis atencion . [Student I1, Female, Session Il] #2: Me quede ponsando en Io que habia dicho antes y...lo que he estudiado antes en ciencias sociales. (Student elaborates about what she remembers.) No puse mucha atencion por estar pensando en eso todo...Y despues comence a poner atencion y me concentre en eso. [Student #1, Female, Session i] #i: Eso fue lo unico quo puse atenci)n. Porque Jo demas no

  8. Octanol/water partition coefficient (logP), topological indices (χ,J ...

    Indian Academy of Sciences (India)

    WINTEC

    indices (ENH2, ES, ESO, ESO2, ECH3, EOCH3, ETCN, ECl, EBr) of AASBN analogs for training set. S.No. R. R' logP χ. J ENH2. ES. ESO. ESO2. ETCN. ECH3. ECl. EBr. 1. H. S 3.37 8.720 1.919 5.750 1.550 0.000 0.000 11.172 0.000 0.000 0.000. 2. 2-OCH3. S 3.24 9.668 1.983 5.789 1.477 0.000 0.000 11.264 0.000 0.000 ...

  9. Europe to the stars

    CERN Document Server

    Schilling, Govert

    2013-01-01

    The creation of the European Southern Observatory (ESO) in 1962 was the culmination of the dream of leading astronomers from five European countries. Over the years, as more member states joined, ESO constructed the La Silla and Paranal observatories, as well as the Atacama Large Millimeter/submillimeter Array (ALMA) together with partners. ESO is now starting to build the world's biggest eye on the sky, the European Extremely Large Telescope. At the dawn of 2012, its 50th anniversary year, ESO is ready to enter a new era. One that not even its founding members could have antici

  10. The jewel on the mountaintop the European Southern Observatory through fifty years

    CERN Document Server

    Madsen, Claus

    2013-01-01

    Authored by ESO senior advisor Claus Madsen, the present book comprises 576 action-packed pages of ESO history and dramatic stories about the people behind the organisation. This is the ultimate historical account about ESO and its telescopes in the southern hemisphere, but also about a truly remarkable European success story in research. Spanning the range from the first telescopes to the future platforms of the next generation, it shows how the improvement of the telescopes leads to a continuously changing view of the Universe. With 150 photos and illustrations. Produced especially for ESO's

  11. O cuidar de crianças portadoras de bexiga neurogênica: representações sociais das necessidades dessas crianças e suas mães El cuidar niños portadores de vejiga neurogenica: representaciones sociales de las necesidades de esos niños y sus madres Caring for children with neurogenic blader dysfunction: social representation of these children's and their mothers' needs

    Directory of Open Access Journals (Sweden)

    Maria de Fátima Farinha Martins Furlan

    2003-12-01

    Full Text Available Esta investigação buscou conhecer e analisar as representações e necessidades de crianças em idade escolar, portadoras de bexiga neurogênica, e de suas mães. A pesquisa foi de natureza qualitativa, sendo as representações sociais o processo metodológico adotado. Na interposição das categorias empíricas com as analíticas, o viver e o cuidar são representados respectivamente pelas mães como um "problema" e "tudo eu"; as crianças, por sua vez, interpretam com "num é justo" a interação diferenciada e preconceituosa que sofrem no contexto em que estão inseridas.Esta investigación busca conocer y analizar las representaciones y necesidades de niños en edad escolar, portadores de vejiga neurogénica y sus madres. Investigación de naturaleza cualitativa, siendo las representaciones sociales el proceso metodológico adoptado. La interposición de las categorías empíricas con las analíticas, el vivir y el cuidar son representados respectivamente por las madres como un "problema" y "todo yo"; los niños, a su vez, interpretan como "no es justa" la interacción diferenciada y con preconceptos que sufren en el contexto en el cual estos niños se desenvuelven cotidianamente.The purpose of this study was to know and analyze the representations and needs of school-age children with neurogenic bladder dysfunction and of their mothers. This is a qualitative study; whose methodology was based on social representations. According to the interposition of empirical end analytical categories, the living and caring are respectively represented by mothers as "a problem" and "all me"; the children, in turn, interpret the differentiated and prejudiced interaction they suffer in the setting they live in as "it is not fair".

  12. The design of the MOONS-VLT spectrometer

    NARCIS (Netherlands)

    Oliva, E.; Diolaiti, E.; Garilli, B.; Gratton, R.; Lorenzetti, D.; Schipani, P.; Scuderi, S.; Vanzella, E.; Cirasuolo, M.; Afonso, J.; Bender, R.; Bonifacio, P.; Kaper, L.; Vanzi, L.; Baffa, C.; Bianco, A.; Bonoli, C.; Bortoletto, F.; Bruno, P.; Carbonaro, L.; Centrone, M.; Cresci, G.; De Caprio, V.; Del Vecchio, C.; Di Marcantonio, P.; Di Paola, A.; D'Alessio, F.; D'Alessandro, M.; D'Orsi, S.; Falcini, G.; Ferruzzi, D.; Fontana, A.; Foppiani, I.; Fumana, M.; Giani, E.; Leone, F.; Li Causi, G.; Lombini, M.; Maiolino, R.; Mannucci, F.; Marty, L.; Miglietta, L.; Munari, M.; Navarro, R.; Origlia, L.; Paioro, L.; Pedichini, F.; Pragt, J.; Randich, S.; Scodeggio, M.; Spano, P.; Speziali, R.; Stuik, R.; Tozzi, A.; Vitali, F.

    2012-01-01

    MOONS is a new conceptual design for a multi-object spectrograph for the ESO Very Large Telescope (VLT) which will provide the ESO astronomical community with a powerful, unique instrument able to serve a wide range of Galactic, Extragalactic and Cosmological studies. The instrument foresees 1000

  13. Procedimiento para la preparación de ácido oregánico

    OpenAIRE

    Noheda Marín, Pedro; Lozano Gordillo, Luis Miguel; Maroto Quintana, Sergio

    2008-01-01

    [ES] La invención se refiere a un método para obtener el ácido y derivados oreganic de eso, a los compuestos intermedios de esta síntesis y al uso de los compuestos dichos en la preparación del ácido y de los derivados oreganic de eso.

  14. OmegaCAM: the 16k × 16k Survey Camera for the VLT Survey Telescope

    NARCIS (Netherlands)

    Deul, Erik; Kuijken, Konrad; Valentijn, Edwin A.; Tyson, J. Anthony; Wolff, Sidney

    2002-01-01

    OmegaCAM, a 16k×16k-pixel wide field optical camera, and the VLT Survey Telescope (VST) that is to host it, will constitute a major sky surveying machine that becomes operational in 2004 at ESO"s Paranal Observatory. It maps one square degree of sky with 0.21 arcsec sized pixels. Both individual

  15. Fluoroantimonic acid hexahydrate (HSbF6-6H2O) catalysis: The ring-opening polymerization of epoxidized soybean oil

    Science.gov (United States)

    Ring-opening polymerization of epoxidized soybean oil (ESO) catalyzed by a super acid, fluroantimonic acid hexahydrate (HSbF6-6H2O), in ethyl acetate was conducted in an effort to develop useful biodegradable polymers. The resulting polymerized ESO (SA-RPESO) were characterized by using infrared (IR...

  16. The DART imaging and CaT survey of the Fornax dwarf spheroidal galaxy

    NARCIS (Netherlands)

    Battaglia, G.; Tolstoy, E.; Helmi, A.; Irwin, M. J.; Letarte, B.; Jablonka, P.; Hill, V.; Venn, K. A.; Shetrone, M. D.; Arimoto, N.; Primas, F.; Kaufer, A.; Francois, P.; Szeifert, T.; Abel, T.; Sadakane, K.

    2006-01-01

    Aims. As part of the DART project we have used the ESO ESO/2.2m Wide Field Imager in conjunction with the VLT/FLAMES(star star) GIRAFFE spectrograph to study the detailed properties of the resolved stellar population of the Fornax dwarf spheroidal galaxy out to and beyond its tidal radius. Fornax

  17. Collaborative learning in a culturally diverse secondary vocational education

    NARCIS (Netherlands)

    Dr. Rutger van de Sande; Drs. Kennedy Aquilino Tielman; Dr. S. Bolhuis; Prof. dr. Perry den Brok

    2010-01-01

    Collaborative learning in a culturally diverse secondary vocational education. By K. Tielman (Fontys), P. den Brok (ESoE), S. Bolhuis (Fontys) and R. van de Sande (Fontys) This contribution discusses a descriptive study on the experiences of students and teachers in secondary vocational education

  18. A novel HLA-B18 restricted CD8+ T cell epitope is efficiently cross-presented by dendritic cells from soluble tumor antigen.

    Directory of Open Access Journals (Sweden)

    Rona Y Zhao

    Full Text Available NY-ESO-1 has been a major target of many immunotherapy trials because it is expressed by various cancers and is highly immunogenic. In this study, we have identified a novel HLA-B*1801-restricted CD8(+ T cell epitope, NY-ESO-1(88-96 (LEFYLAMPF and compared its direct- and cross-presentation to that of the reported NY-ESO-1(157-165 epitope restricted to HLA-A*0201. Although both epitopes were readily cross-presented by DCs exposed to various forms of full-length NY-ESO-1 antigen, remarkably NY-ESO-1(88-96 is much more efficiently cross-presented from the soluble form, than NY-ESO-1(157-165. On the other hand, NY-ESO-1(157-165 is efficiently presented by NY-ESO-1-expressing tumor cells and its presentation was not enhanced by IFN-γ treatment, which induced immunoproteasome as demonstrated by Western blots and functionally a decreased presentation of Melan A(26-35; whereas NY-ESO-1(88-96 was very inefficiently presented by the same tumor cell lines, except for one that expressed high level of immunoproteasome. It was only presented when the tumor cells were first IFN-γ treated, followed by infection with recombinant vaccinia virus encoding NY-ESO-1, which dramatically increased NY-ESO-1 expression. These data indicate that the presentation of NY-ESO-1(88-96 is immunoproteasome dependent. Furthermore, a survey was conducted on multiple samples collected from HLA-B18(+ melanoma patients. Surprisingly, all the detectable responses to NY-ESO-1(88-96 from patients, including those who received NY-ESO-1 ISCOMATRIX™ vaccine were induced spontaneously. Taken together, these results imply that some epitopes can be inefficiently presented by tumor cells although the corresponding CD8(+ T cell responses are efficiently primed in vivo by DCs cross-presenting these epitopes. The potential implications for cancer vaccine strategies are further discussed.

  19. Separation of toluene from alkanes using 1-ethyl-3-methylpyridinium ethylsulfate ionic liquid at T = 298.15 K and atmospheric pressure

    International Nuclear Information System (INIS)

    Gonzalez, Emilio J.; Calvar, Noelia; Gonzalez, Begona; Dominguez, Angeles

    2010-01-01

    In this paper, the separation of toluene from aliphatic hydrocarbons (heptane, or octane, or nonane) was analyzed by solvent extraction with 1-ethyl-3-methylpyridinium ethylsulfate ionic liquid, [EMpy][ESO 4 ]. Liquid-liquid equilibrium (LLE) data for the ternary systems {heptane (1) + toluene (2) + [EMpy][ESO 4 ] (3)}, {octane (1) + toluene (2) + [EMpy][ESO 4 ] (3)}, and {nonane (1) + toluene (2) + [EMpy][ESO 4 ] (3)} were obtained by measurements at T = 298.15 K and atmospheric pressure. The selectivity, % removal of aromatic, and solute distribution ratio, obtained from experimental equilibrium results, were used to determine the ability of [EMpy][ESO 4 ] as a solvent. The degree of consistency of the experimental LLE values was ascertained using the Othmer-Tobias and Hand equations. The experimental results for the ternary systems were correlated with the NRTL model. Finally, the results obtained were compared with other ionic liquids and other solvents.

  20. 40+ Years of Instrumentation for the La Silla Paranal Observatory

    Science.gov (United States)

    D'Odorico, S.

    2018-03-01

    As ESO Period 100 comes to a close, I look back at the development of ESO's instrumentation programme over more than 40 years. Instrumentation and detector activities were initially started by a small group of designers, engineers, technicians and astronomers while ESO was still at CERN in Geneva in the late 1970s. They have since led to the development of a successful suite of optical and infrared instruments for the La Silla Paranal Observatory, as testified by the continuous growth in the number of proposals for observing time and in the publications based on data from ESO telescopes. The instrumentation programme evolved significantly with the VLT and most instruments were developed by national institutes in close cooperation with ESO. This policy was a cornerstone of the VLT programme from the beginning and a key to its success.

  1. Thermal properties, curing characteristics and water absorption of soybean oil-based thermoset

    Directory of Open Access Journals (Sweden)

    2011-06-01

    Full Text Available Epoxidized soybean oil (ESO was successfully thermal-cured by using methylhexahydrophthalic anhydride (MHHPA curing agent, in the presence of tetraethylammonium bromide (TEAB catalyst of varied concentration (0.3–0.8 phr. The polyesterification process of ESO thermoset was proven and supported by Fourier transforms infrared spectroscopy (FTIR and gas chromatography-mass spectroscopy analysis (GC-MS. A possible chemical reaction of the MHHPA, TEAB and ESO was proposed based on the experimental work. Differential scanning calorimetry (DSC and dynamic mechanical analysis (DMA revealed that there is a positive relationship between the degree of conversion and crosslink density of ESO thermoset with TEAB concentration. The kinetics of water absorption of the ESO thermoset were found to conform to Fickian law behavior.

  2. Does Employee Stock Ownership Work?

    DEFF Research Database (Denmark)

    Kato, Takao; Miyajima, Hideaki; Owan, Hideo

    This paper provides novel evidence on the effects of employee stock ownership (ESO), using new panel data on Japanese ESO plans for a highly representative sample of publicly-traded firms in Japan (covering more than 75% of all firms listed on Tokyo Stock Exchange) over 1989-2013. Unlike most prior...... studies, we focus on the effects of changes in varying attributes of existing ESO—the effects on the intensive margin. Our fixed effect estimates show that an increase in the strength of the existing ESO plans measured by stake per employee results in statistically significant productivity gains...... of the existing core participants is more effective in boosting gains from ESO plans than bringing in more employees into the trust. Reassuringly, our key results are found to be robust to the use of instrumental variables to account for possible endogeneity of ESO plans. Finally, we explore possible interplays...

  3. Effect of epoxidised soybean oil loading as plasticiser on physical, mechanical and thermal properties of polyvinylchloride

    Science.gov (United States)

    Rahmah, M.; Nurazzi, N. Mohd; Farah Nordyana, A. R.; Syed Anas, S. M.

    2017-07-01

    The aim of this paper is to study the effect of epoxidised soybean oil (ESO) as an alternative plasticizer on physical, mechanical and thermal properties of plasticised polyvinyl chloride (PPVC). Samples were prepared using 10, 20, 30 and 40% by weight percent of ESO. The samples were characterized for density, water absorption, tensile, hardness and thermal properties. The addition of ESO as plasticizer in PVC had caused significant effect on the physical and mechanical properties of PPVC. Increasing of ESO loading had resulted in decreased density, tensile strength, tensile modulus but increased in elongation at break and shore hardness. From water absorption study, it was observed that the all the samples reached the plateau absorption at days 8 to 10 with absorption percentages of between 1.8 to 2%. In general the crystallinity of PPVC maintained between 10 to 13% with increase in ESO loading while the melting point ( Tm) is slightly decreased about 3 to 6°C. In this study, ESO which acts as plasticiser were found to result in lower glass transition temperature (Tg). The enhancements of super cooling with higher ESO loading were found to increase the crystallization temperature, promoting crystallisation and act as nucleating agent.

  4. "Green" films from renewable resources: properties of epoxidized soybean oil plasticized ethyl cellulose films.

    Science.gov (United States)

    Yang, Dong; Peng, Xinwen; Zhong, Linxin; Cao, Xuefei; Chen, Wei; Zhang, Xueming; Liu, Shijie; Sun, Runcang

    2014-03-15

    Epoxidized soybean oil (ESO), which is a biomass-derived resource, was first used as a novel plasticizer for ethyl cellulose (EC) film preparation. Surface morphologies, mechanical performances, thermal properties, oxygen and water vapor permeabilities of plasticized EC films were detected in detail to evaluate the plasticizing effect of ESO and explore the plastication mechanisms. Results showed that ESO was an effective plasticizer that outstripped conventional plasticizers, i.e. dibutyl phthalate (DBP) and triethyl citrate (TEC) in producing high-quality films. Especially, at plasticizer concentrations of 15-25%, ESO-EC films had preferable mechanical properties and better thermal stability, as well as non-flammability. In addition, the water vapor permeability of ESO-EC films was lower than that of traditional plasticized films. Their oxygen permeability was also remained in a low level. These outstanding performances were related to the relatively high molecular weight, hydrophobicity, chemical structure of ESO, and the intermolecular interactions between ESO and EC chains. Copyright © 2013 Elsevier Ltd. All rights reserved.

  5. Catching Galactic open clusters in advanced stages of dynamical evolution

    Science.gov (United States)

    Angelo, M. S.; Piatti, A. E.; Dias, W. S.; Maia, F. F. S.

    2018-04-01

    During their dynamical evolution, Galactic open clusters (OCs) gradually lose their stellar content mainly because of internal relaxation and tidal forces. In this context, the study of dynamically evolved OCs is necessary to properly understand such processes. We present a comprehensive Washington CT1 photometric analysis of six sparse OCs, namely: ESO 518-3, Ruprecht 121, ESO 134-12, NGC 6573, ESO 260-7 and ESO 065-7. We employed Markov chain Monte-Carlo simulations to robustly determine the central coordinates and the structural parameters and T1 × (C - T1) colour-magnitude diagrams (CMDs) cleaned from field contamination were used to derive the fundamental parameters. ESO 518-03, Ruprecht 121, ESO 134-12 and NGC 6573 resulted to be of nearly the same young age (8.2 ≤log(t yr-1) ≤ 8.3); ESO 260-7 and ESO065-7 are of intermediate age (9.2 ≤log(t yr-1) ≤ 9.4). All studied OCs are located at similar Galactocentric distances (RG ˜ 6 - 6.9 kpc), considering uncertainties, except for ESO 260-7 (RG = 8.9 kpc). These OCs are in a tidally filled regime and are dynamically evolved, since they are much older than their half-mass relaxation times (t/trh ≳ 30) and present signals of low-mass star depletion. We distinguished two groups: those dynamically evolving towards final disruptions and those in an advanced dynamical evolutionary stage. Although we do not rule out that the Milky Way potential could have made differentially faster their dynamical evolutions, we speculate here with the possibility that they have been mainly driven by initial formation conditions.

  6. Ultra-luminous X-ray sources and intermediate-mass black holes

    International Nuclear Information System (INIS)

    Cseh, David

    2012-01-01

    use these nebulae as a calorimeters to infer the total intrinsic power of these ULXs, characterize the feedback on the environment, and compare to Galactic sources. I also investigated the power mechanism of inflating a bubble by jets. Then I discuss the current status of radio observations on ULXs, including ongoing radio surveys. Finally, I studied HLX-1, which is likely the first bona fide IMBH. This involves radio observations and analysis of the radio data that led to a discovery of radio flares from this source during an X-ray state transition. From the strength of the flares, I provided additional constraints on the mass of the black hole and its accretion rate. (author) [fr

  7. Lo trágico y la lástima. “Discurso de respuesta a René Girard”

    OpenAIRE

    Palau Castaño, Luis Alfonso; Universidad de Medellín

    2015-01-01

    ¿De dónde llegan hasta acá esos ladridos? ¿Reconocemos igualmente en el relato de Theramen, los caballos desbocados que arrastran el cadáver de Hi­pólito por la playa, descuartizado? ¿Quiénes son estas serpientes que silban sobre vuestras cabezas? Gracias, señor, por habernos hecho escuchar, en esos ladridos, esos relinchos, estos aullidos de animales rabiosos, nuestras propias vociferaciones; de haber desvelado, en esta jauría sangrienta, en esta yunta desbocada, en este nudo de víboras, en ...

  8. Acceso y uso de nuevas tecnologías entre los jóvenes de educación secundaria, implicaciones en salud. Estudio JOITIC

    OpenAIRE

    Raquel Muñoz-Miralles; Raquel Ortega-González; Carme Batalla-Martínez; María Rosa López-Morón; Josep Maria Manresa; Pere Torán-Monserrat

    2014-01-01

    Objetivo: Determinar la accesibilidad y el uso de las Tecnologías de la Información y la Comunicación (TIC) entre los jóvenes de Educación Secundaria Obligatoria (ESO) y analizar factores relacionados. Diseño Estudio descriptivo multicéntrico transversal. Emplazamiento: Institutos de ESO participantes en el programa «Salut i Escola». Participantes: Un total de 5.538 alumnos de primero a cuarto curso de ESO de 28 centros del área de Barcelona. Mediciones principales: Aplicación de ...

  9. Brazil to Join the European Southern Observatory

    Science.gov (United States)

    2010-12-01

    The Federative Republic of Brazil has yesterday signed the formal accession agreement paving the way for it to become a Member State of the European Southern Observatory (ESO). Following government ratification Brazil will become the fifteenth Member State and the first from outside Europe. On 29 December 2010, at a ceremony in Brasilia, the Brazilian Minister of Science and Technology, Sergio Machado Rezende and the ESO Director General, Tim de Zeeuw signed the formal accession agreement aiming to make Brazil a Member State of the European Southern Observatory. Brazil will become the fifteen Member State and the first from outside Europe. Since the agreement means accession to an international convention, the agreement must now be submitted to the Brazilian Parliament for ratification [1]. The signing of the agreement followed the unanimous approval by the ESO Council during an extraordinary meeting on 21 December 2010. "Joining ESO will give new impetus to the development of science, technology and innovation in Brazil as part of the considerable efforts our government is making to keep the country advancing in these strategic areas," says Rezende. The European Southern Observatory has a long history of successful involvement with South America, ever since Chile was selected as the best site for its observatories in 1963. Until now, however, no non-European country has joined ESO as a Member State. "The membership of Brazil will give the vibrant Brazilian astronomical community full access to the most productive observatory in the world and open up opportunities for Brazilian high-tech industry to contribute to the European Extremely Large Telescope project. It will also bring new resources and skills to the organisation at the right time for them to make a major contribution to this exciting project," adds ESO Director General, Tim de Zeeuw. The European Extremely Large Telescope (E-ELT) telescope design phase was recently completed and a major review was

  10. Characterisation of the aerobic bacterial flora of boid snakes: application of MALDI-TOF mass spectrometry.

    Science.gov (United States)

    Plenz, Bastian; Schmidt, Volker; Grosse-Herrenthey, Anke; Krüger, Monika; Pees, Michael

    2015-03-14

    The aim of this study was to identify aerobic bacterial isolates from the respiratory tract of boids with matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry (MALDI-TOF MS). From 47 boid snakes, swabs from the oral cavity, tracheal wash samples and, in cases in which postmortem examination was performed, pulmonary tissue samples were taken. Each snake was classified as having inflammation of the respiratory tract and/or oral cavity, or without evidence of inflammation based on combination of clinical, cytological and histopathological findings. Samples collected from the respiratory tract and oral cavity were inoculated onto routine media and bacteria were cultured aerobically. All morphologically distinct individual colonies obtained were analysed using MALDI-TOF MS. Unidentified isolates detected in more than three snakes were selected for further 16S rDNA PCR and sequencing. Among all examined isolates (n=243), 49 per cent (n=119) could be sufficiently speciated using MALDI-TOF MS. Molecular biology revealed several bacterial species that have not been previously described in reptiles. With an average of 6.3 different isolates from the respiratory tract and/or oral cavity, boids with inflammatory disease harboured significantly more bacterial species than boids without inflammatory disease (average 2.8 isolates). British Veterinary Association.

  11. "Clouds" above Paranal.

    Science.gov (United States)

    1994-04-01

    ESO, the European Southern Observatory, in reply to questions raised by the media would like to clarify its position with regard to recent events which concern the land on which the Paranal mountain is situated. THE DECISION TO BUILD THE VLT AT PARANAL In December 1987, the Council [1] of the European Southern Observatory decided to build the largest optical telescope in the world, the 16-metre equivalent Very Large Telescope (VLT) [2], before the end of the century and at a total cost that was expected to approach 500 million DEM. Already several years before that, ESO had started a search for the best possible site for this new giant telescope. At the time of Council's decision, intensive investigations at various sites in the Chilean Atacama desert had effectively narrowed down the choice to two possibilities, the Vizcachas mountain near La Silla, and the Paranal mountain, located approx. 130 km south of Antofagasta, the capital of the Chilean Region II. The meteorological data measured by the ESO teams favoured Paranal, especially in terms of number of clear nights and amount of turbulence in the atmosphere. However, while Vizcachas is situated on land that had earlier been acquired by ESO, this was not the case for the Paranal mountain. ESO was therefore very pleased to learn in 1988 that the Chilean government had decided to donate an area of 725 sq. km around Paranal to this Organisation, on the condition that it would be decided within the next five years to construct the VLT at this site. The size of this land is dictated by the need to avoid any activities (e.g., mining) which may adversely influence the exceedingly sensitive astronomical observations with the VLT. The offer was gratefully accepted by the ESO Council and in November 1988 ESO became owner of the land. After further detailed considerations of the scientific and technical implications, the ESO Council during its December 1990 meeting decided to construct the VLT on Paranal [3], thus

  12. European Southern Observatory

    CERN Multimedia

    CERN PhotoLab

    1970-01-01

    Professor A. Blaauw, Director general of the European Southern Observatory, with George Hampton on his right, signs the Agreement covering collaboration with CERN in the construction of the large telescope to be installed at the ESO Observatory in Chile.

  13. La physique à l'honneur

    CERN Document Server

    2000-01-01

    "Physics on stage", a joint project of CERN, ESA and ESO will begin next week. It is an international festival aimed at making physics interesting and fun to try and encourage more children to study it (1 page).

  14. The Pension Fund passes important milestones

    CERN Multimedia

    2012-01-01

    In this column, the Chairman of the Pension Fund Governing Board (PFGB) presents the Board's latest main decisions, initiatives and accomplishments to the Fund's members and beneficiaries.   Since my last report in October, the PFGB has passed several milestones in actuarial, technical and investment matters. The PFGB has completed an analysis of a request by the European Organisation for Astronomical Research in the Southern Hemisphere (ESO) to reduce the increased cost of pension insurance for new ESO recruits that has been caused by the increased CHF/€ exchange ratio. Currently the staff of ESO are admitted to the CERN Pension Fund, pursuant to a co-operation agreement between CERN and ESO dating back to 1968. This analysis assessed the actuarial, financial, administrative and legal implications, and is scheduled to be presented to the CERN Council and the Finance Committee in December. After an open tendering process the PFGB has selected Buck Consultants Limited...

  15. Energy Engineering Analysis Program Study, Fort Leonard Wood, Missouri. Volume 3, Appendices G and H

    National Research Council Canada - National Science Library

    1993-01-01

    ...) Energy Saving Opportunity Survey (ESOS) at Fort Leonard Wood, Missouri. This study was authorized under the contract DACA41-92-C-0098 with Corps of Engineers Kansas City District, Kansas City, Missouri...

  16. Aspects epidemiologiques et diagnostiques des stenoses ...

    African Journals Online (AJOL)

    21.96%). The clinical symptomatologies were dominated by dysphagia (100%). Digestive endoscopy was realised in 83.78% cases and the eso-gastro-duodenal transit in 100% cases. Conclusion: The esophageal caustic's stenoses are still ...

  17. CCD OBSERVATIONS V1.0

    Data.gov (United States)

    National Aeronautics and Space Administration — Charged Coupled Device (CCD) cameras from ESO were used by groups to observe the outburst of comet Halley using a variety of telescopes and chip sets.

  18. VizieR Online Data Catalog: YZ Ceti radial velocity curve (Astudillo-Defru+, 2017)

    Science.gov (United States)

    Astudillo-Defru, N.; Diaz, R. F.; Bonfils, X.; Almenara, J. M.; Delisle, J.-B.; Bouchy, F.; Delfosse, X.; Forveille, T.; Lovis, C.; Mayor, M.; Murgas, F.; Pepe, F.; Santos, N. C.; Segransan, D.; Udry, S.; Wunsche, A.

    2017-08-01

    We acquired spectra with HARPS, a stabilized high-resolution spectrograph specifically designed to compute precise radial velocities. It is an ESO instrument installed at La Silla observatory. (1 data file).

  19. SMALL SOLAR SYSTEM OBJECTS SPECTROSCOPIC SURVEY V1.0

    Data.gov (United States)

    National Aeronautics and Space Administration — This dataset contains the visible spectra of 820 asteroids obtained between November 1996 and May 2001 at the 1.52m telescope at ESO (La Silla). The useful spectral...

  20. Measuring business performance using indicators of ecologically sustainable organizations

    Science.gov (United States)

    Snow, Charles G., Jr.; Snow, Charles C.

    2001-02-01

    The purpose of this paper is to explore the use of ecology-based performance measures as a way of augmenting the Balanced Scorecard approach to organizational performance measurement. The Balanced Scorecard, as proposed by Kaplan and Norton, focuses on four primary dimensions; financial, internal-business-process, customer, and learning and growth perspectives. Recently, many 'green' organizational theorists have developed the concept of "Ecologically Sustainable Organizations" or ESOs, a concept rooted in open systems theory. The ESO is called upon to consider resource use and conservation as a strategy for long-term viability. This paper asserts that in order to achieve ESO status, an organization must not only measure but also reward resource conservation measures. Only by adding a fifth perspective for ecological dimensions will the entity be truly motivated toward ESO status.

  1. La physique et la vie: Quand des enseignants rencontrent des chercheurs, à l'initiative d'EIROforum

    CERN Multimedia

    CERN Press Office. Geneva

    2003-01-01

    More than 400 selected delegates from 22 European countries will take part in Physics on Stage 3, organised by the EIROforum research organisations (CERN, EFDA, EMBL, ESA, ESO, ESRF, ILL) at ESA's ESTEC site in The Netherlands (1 page).

  2. Page 1 478 S Bandopadhyay et al Binding energy (eV) 84 O 85 O ...

    Indian Academy of Sciences (India)

    Figure 6. Expanded runs of the strongest XPS core lines, a. Ni2p doublet and b. Si2P. Table 1. Composition of nickel silicide film. XPS Amplitude FWHM Intensity ASF Relative atomic line (A;KCPS) [T;eV] (I, Kcps-eV) (si) concentration (C). Ni2pala (Eso = 852-6 eV) 135-2 2-4 324.5. Main line 3.7 0.49. Satellite. Eso = 857.8 ...

  3. New York esophageal squamous cell carcinoma-1 and cancer immunotherapy.

    Science.gov (United States)

    Esfandiary, Ali; Ghafouri-Fard, Soudeh

    2015-01-01

    New York esophageal squamous cell carcinoma 1 (NY-ESO-1) is a known cancer testis gene with exceptional immunogenicity and prevalent expression in many cancer types. These characteristics have made it an appropriate vaccine candidate with the potential application against various malignancies. This article reviews recent knowledge about the NY-ESO-1 biology, function, immunogenicity and expression in cancers as well as and the results of clinical trials with this antigen.

  4. IOT Overview: Wide-Field Imaging

    Science.gov (United States)

    Selman, F. J.

    The Wide Field Imager (WFI) instrument at La Silla has been the workhorse of wide-field imaging instruments at ESO for several years. In this contribution I will summarize the issues relating to its productivity for the community both in terms of the quality and quantity of data that has come out of it. Although only surveys of limited scope have been completed using WFI, it is ESO's stepping-stone to the new generation of survey telescopes.

  5. IOT Overview: Optical Spectro-Imagers

    Science.gov (United States)

    Patat, F.

    Taking the FORS instruments as a representative case, I review the Calibration Plan for optical spectro-imagers currently offered at ESO, discussing various aspects related both to the scientific outcome and the instrument/site monitoring. I also describe ongoing and future calibration projects planned by the Instrument Operations Teams, trying to give an objective view on the limitations of the Calibration Plans currently implemented at ESO for this class of instruments.

  6. GOVERNING BOARD OF THE PENSION FUND

    CERN Document Server

    2004-01-01

    The Governing Board held its 120th and 121st meetings on 4 November and 2 December 2003 respectively. At the first of these two meetings, the Board first continued its examination of ESO's requests. In connection to this, the Board heard a presentation of the requests by M. Bloch, ESO's Head of Personnel, including a proposal that ESO's contributions and benefits to its beneficiaries be paid in euros. This option had previously been examined by the Working Group on ESO's Requests which had submitted a negative opinion to the Governing Board. Mr Bloch informed the Board that ESO was suspending that request and therefore invited the Governing Board to concentrate on the other options. After some discussion, the Governing Board decided that the euro-based request should be deemed withdrawn and that the Working Group would resume its examination of the other options put forward by ESO at the beginning of the year. At the same meeting, J.-P. Matheys reported on the recent meeting of the Working Group on Actuarial...

  7. Toughening of nanocelluose/PLA composites via bio-epoxy interaction: Mechanistic study

    Energy Technology Data Exchange (ETDEWEB)

    Meng, Xiangtao [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bocharova, Vera [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Tekinalp, Halil L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States); Cheng, Shiwang [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Kisliuk, Alexander [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Sokolov, Alexei P. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Kunc, Vlastimil [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Peter, William H. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Ozcan, Soydan [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

    2017-11-07

    While PLA possesses modest to good strength and stiffness, broader application is hindered by its brittle nature. The aim of this study was to develop strong and tough polymeric materials from renewable biomaterials and understand the underlying interactions and mechanisms. Cellulose nanofibrils (CNFs) and epoxidized soybean oil (ESO) were compounded with poly(lactic acid) (PLA) to create a PLA-CNF-ESO tertiary nanocomposite system. Tensile and dynamic mechanical analyses were performed to see how variations in ESO and CNF content affect mechanical properties such as strength, modulus, ductility, and toughness. It was found that at low CNF levels (10 wt %) the addition of ESO can improve the ductility of the nanocomposites 5- to 10-fold with only slight losses in strength and modulus, while at higher CNF levels (20 and 30 wt %), ESO exhibited little effect on mechanical properties, possibly due to percolation of CNFs in the matrix, dominating stress transfer. Therefore, it is important to optimize CNF and ESO amounts in composites to achieve materials with both high strength and high toughness. As a result, efforts have been made to understand the underlying mechanisms of the mechanical behavior of one class of these composites via thermal, dynamic mechanical, morphological, and Raman analyses.

  8. Clear New View of a Classic Spiral

    Science.gov (United States)

    2010-05-01

    ESO is releasing a beautiful image of the nearby galaxy Messier 83 taken by the HAWK-I instrument on ESO's Very Large Telescope (VLT) at the Paranal Observatory in Chile. The picture shows the galaxy in infrared light and demonstrates the impressive power of the camera to create one of the sharpest and most detailed pictures of Messier 83 ever taken from the ground. The galaxy Messier 83 (eso0825) is located about 15 million light-years away in the constellation of Hydra (the Sea Serpent). It spans over 40 000 light-years, only 40 percent the size of the Milky Way, but in many ways is quite similar to our home galaxy, both in its spiral shape and the presence of a bar of stars across its centre. Messier 83 is famous among astronomers for its many supernovae: vast explosions that end the lives of some stars. Over the last century, six supernovae have been observed in Messier 83 - a record number that is matched by only one other galaxy. Even without supernovae, Messier 83 is one of the brightest nearby galaxies, visible using just binoculars. Messier 83 has been observed in the infrared part of the spectrum using HAWK-I [1], a powerful camera on ESO's Very Large Telescope (VLT). When viewed in infrared light most of the obscuring dust that hides much of Messier 83 becomes transparent. The brightly lit gas around hot young stars in the spiral arms is also less prominent in infrared pictures. As a result much more of the structure of the galaxy and the vast hordes of its constituent stars can be seen. This clear view is important for astronomers looking for clusters of young stars, especially those hidden in dusty regions of the galaxy. Studying such star clusters was one of the main scientific goals of these observations [2]. When compared to earlier images, the acute vision of HAWK-I reveals far more stars within the galaxy. The combination of the huge mirror of the VLT, the large field of view and great sensitivity of the camera, and the superb observing conditions

  9. Preparation and characterization of fast dissolving flurbiprofen and esomeprazole solid dispersion using spray drying technique.

    Science.gov (United States)

    Pradhan, Roshan; Tran, Tuan Hiep; Kim, Sung Yub; Woo, Kyu Bong; Choi, Yong Joo; Choi, Han-Gon; Yong, Chul Soon; Kim, Jong Oh

    2016-04-11

    We aimed to develop an immediate-release flurbiprofen (FLU) and esomeprazole (ESO) combination formulation with enhanced gastric aqueous solubility and dissolution rate. Aqueous solubility can be enhanced by formulating solid dispersions (SDs) with a polyvinylpyrrolidone (PVP)-K30 hydrophilic carrier, using spray-drying technique. Aqueous and gastric pH dissolution can be achieved by macro-environmental pH modulation using sodium bicarbonate (NaHCO3) and magnesium hydroxide (Mg(OH)2) as the alkaline buffer. FLU/ESO-loaded SDs (FLU/ESO-SDs) significantly improved aqueous solubility of both drugs, compared to each drug powder. Dissolution studies in gastric pH and water were compared with the microenvironmental pH modulated formulations. The optimized FLU/ESO-SD powder formulation consisted of FLU/ESO/PVP-K30/sodium carbonate (Na2CO3) in a weight ratio 1:0.22:1.5:0.3, filled in the inner capsule. The outer capsule consisted of NaHCO3 and Mg(OH)2, which created the macro-environmental pH modulation. Increased aqueous and gastric pH dissolution of FLU and ESO from the SD was attributed to the alkaline buffer effects and most importantly, to drug transformation from crystalline to amorphous SD powder, clearly revealed by scanning electron microscopy, differential scanning calorimetry, and powder X-ray diffraction studies. Thus, the combined FLU and ESO SD powder can be effectively delivered as an immediate-release formulation using the macro-environmental pH modulation concept. Copyright © 2016. Published by Elsevier B.V.

  10. What Kind of Capsule Endoscope Is Suitable for a Controllable Self-Propelling Capsule Endoscope? Experimental Study Using a Porcine Stomach Model for Clinical Application (with Videos).

    Science.gov (United States)

    Ota, Kazuhiro; Nouda, Sadaharu; Takeuchi, Toshihisa; Iguchi, Munetaka; Kojima, Yuichi; Kuramoto, Takanori; Inoue, Takuya; Shindo, Yasunori; Uesugi, Kenshiro; Fujito, Yoshiaki; Nishihara, Hironori; Ohtsuka, Naotake; Higuchi, Kazuhide

    2015-01-01

    We have been developing the Self-Propelling Capsule Endoscope (SPCE) that allows for controllability from outside of the body and real-time observation. What kind of capsule endoscope (CE) is suitable for a controllable SPCE is unclear and a very critical point for clinical application. We compared observing ability of three kinds of SPCEs with different viewing angles and frame rates. Eleven buttons were sewed in an excised porcine stomach. Four examiners controlled the SPCE using PillCamSB2, -ESO2, and -COLON2 (Given Imaging Ltd., Israel), for 10 minutes each with the aim of detecting as many buttons and examining them as closely as possible. The ability to find lesions was assessed based on the number of detected buttons. The SPCE-performance score (SPS) was used to evaluate the ability to examine the lesions in detail. The SPCE-ESO2, -COLON2, and -SB2 detected 11 [interquartile range (IQR): 0], 10.5 (IQR, 0.5), and 8 (IQR, 1.0) buttons, respectively. The SPCE-ESO2 and -COLON2 had a significantly better ability to detect lesions than the -SB2 (p < 0.05). The SPCE-ESO2, -COLON2, and -SB2 had significantly different SPS values of 22 (IQR, 0), 16.5 (IQR, 1.5), and 14 (IQR, 1.0), respectively (p < 0.05 for all comparisons; SPCE-SB2 vs. -ESO2, -SB2 vs. -COLON2, and -ESO2 vs. -COLON2). PillCamESO2 is most suitable in different three CEs for SPCE for examining lesions in detail of the stomach.

  11. Tuning excited state isomerization dynamics through ground state structural changes in analogous ruthenium and osmium sulfoxide complexes.

    Science.gov (United States)

    Garg, Komal; Engle, James T; Ziegler, Christopher J; Rack, Jeffrey J

    2013-08-26

    The complexes [Ru(bpy)2(pyESO)](PF6)2 and [Os(bpy)2(pyESO)](PF6)2, in which bpy is 2,2'-bipyridine and pyESO is 2-((isopropylsulfinyl)ethyl)pyridine, were prepared and studied by (1)H NMR, UV-visible and ultrafast transient absorption spectroscopy, as well as by electrochemical methods. Crystals suitable for X-ray structural analysis were grown for [Ru(bpy)2(pyESO)](PF6)2. Cyclic voltammograms of both complexes provide evidence for S→O and O→S isomerization as these voltammograms are described by an ECEC (electrochemical-chemical electrochemical-chemical) mechanism in which isomerization follows Ru(2+) oxidation and Ru(3+) reduction. The S- and O-bonded Ru(3+/2+) couples appear at 1.30 and 0.76 V versus Ag/AgCl in propylene carbonate. For [Os(bpy)2(pyESO)](PF6)2, these couples appear at 0.97 and 0.32 V versus Ag/AgCl in acetonitrile, respectively. Charge-transfer excitation of [Ru(bpy)2(pyESO)](PF6)2 results in a significant change in the absorption spectrum. The S-bonded isomer of [Ru(bpy)2(pyESO)](2+) features a lowest energy absorption maximum at 390 nm and the O-bonded isomer absorbs at 480 nm. The quantum yield of isomerization in [Ru(bpy)2(pyESO)](2+) was found to be 0.58 in propylene carbonate and 0.86 in dichloroethane solution. Femtosecond transient absorption spectroscopic measurements were collected for both complexes, revealing time constants of isomerizations of 81 ps (propylene carbonate) and 47 ps (dichloroethane) in [Ru(bpy)2(pyESO)](2+). These data and a model for the isomerizing complex are presented. A striking conclusion from this analysis is that expansion of the chelate ring by a single methylene leads to an increase in the isomerization time constant by nearly two orders of magnitude. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. HUBBLE PHOTOGRAPHS WARPED GALAXY AS CAMERA PASSES MILESTONE

    Science.gov (United States)

    2002-01-01

    NASA's Hubble Space Telescope has captured an image of an unusual edge-on galaxy, revealing remarkable details of its warped dusty disk and showing how colliding galaxies spawn the formation of new generations of stars. The dust and spiral arms of normal spiral galaxies, like our own Milky Way, appear flat when viewed edge-on. This month's Hubble Heritage image of ESO 510-G13 shows a galaxy that, by contrast, has an unusual twisted disk structure, first seen in ground-based photographs obtained at the European Southern Observatory (ESO) in Chile. ESO 510-G13 lies in the southern constellation Hydra, roughly 150 million light-years from Earth. Details of the structure of ESO 510-G13 are visible because the interstellar dust clouds that trace its disk are silhouetted from behind by light from the galaxy's bright, smooth central bulge. The strong warping of the disk indicates that ESO 510-G13 has recently undergone a collision with a nearby galaxy and is in the process of swallowing it. Gravitational forces distort the structures of the galaxies as their stars, gas, and dust merge together in a process that takes millions of years. Eventually the disturbances will die out, and ESO 510-G13 will become a normal-appearing single galaxy. In the outer regions of ESO 510-G13, especially on the right-hand side of the image, we see that the twisted disk contains not only dark dust, but also bright clouds of blue stars. This shows that hot, young stars are being formed in the disk. Astronomers believe that the formation of new stars may be triggered by collisions between galaxies, as their interstellar clouds smash together and are compressed. The Heritage Team used Hubble's Wide Field Planetary Camera 2 (WFPC2) to observe ESO 510-G13 in April 2001. Pictures obtained through blue, green, and red filters were combined to make this color-composite image, which emphasizes the contrast between the dusty spiral arms, the bright bulge, and the blue star-forming regions. During the

  13. Nanopolymers improve delivery of exon skipping oligonucleotides and concomitant dystrophin expression in skeletal muscle of mdx mice

    Directory of Open Access Journals (Sweden)

    Sirsi Shashank R

    2008-04-01

    Full Text Available Abstract Background Exon skipping oligonucleotides (ESOs of 2'O-Methyl (2'OMe and morpholino chemistry have been shown to restore dystrophin expression in muscle fibers from the mdx mouse, and are currently being tested in phase I clinical trials for Duchenne Muscular Dystrophy (DMD. However, ESOs remain limited in their effectiveness because of an inadequate delivery profile. Synthetic cationic copolymers of poly(ethylene imine (PEI and poly(ethylene glycol (PEG are regarded as effective agents for enhanced delivery of nucleic acids in various applications. Results We examined whether PEG-PEI copolymers can facilitate ESO-mediated dystrophin expression after intramuscular injections into tibialis anterior (TA muscles of mdx mice. We utilized a set of PEG-PEI copolymers containing 2 kDa PEI and either 550 Da or 5 kDa PEG, both of which bind 2'OMe ESOs with high affinity and form stable nanoparticulates with a relatively low surface charge. Three weekly intramuscular injections of 5 μg of ESO complexed with PEI2K-PEG550 copolymers resulted in about 500 dystrophin-positive fibers and about 12% of normal levels of dystrophin expression at 3 weeks after the initial injection, which is significantly greater than for injections of ESO alone, which are known to be almost completely ineffective. In an effort to enhance biocompatibility and cellular uptake, the PEI2K-PEG550 and PEI2K-PEG5K copolymers were functionalized by covalent conjugation with nanogold (NG or adsorbtion of colloidal gold (CG, respectively. Surprisingly, using the same injection and dosing regimen, we found no significant difference in dystrophin expression by Western blot between the NG-PEI2K-PEG550, CG-PEI2K-PEG5K, and non-functionalized PEI2K-PEG550 copolymers. Dose-response experiments using the CG-PEI2K-PEG5K copolymer with total ESO ranging from 3–60 μg yielded a maximum of about 15% dystrophin expression. Further improvements in dystrophin expression up to 20% of normal

  14. Separation of benzene from alkanes using 1-ethyl-3-methylpyridinium ethylsulfate ionic liquid at several temperatures and atmospheric pressure: Effect of the size of the aliphatic hydrocarbons

    International Nuclear Information System (INIS)

    Gonzalez, Emilio J.; Calvar, Noelia; Gomez, Elena; Dominguez, Angeles

    2010-01-01

    The ionic liquid 1-ethyl-3-methylpyridinium ethylsulfate, [EMpy][ESO 4 ], was studied for the separation of benzene from aliphatic hydrocarbons (octane or nonane) by solvent extraction through the determination of the (liquid + liquid) equilibrium (LLE) of the ternary systems: {octane (1) + benzene (2) + [EMpy][ESO 4 ] (3)} and {nonane (1) + benzene (2) + [EMpy][ESO 4 ] (3)} at T = (283.15 and 298.15) K and atmospheric pressure. Binodal curves were determined using the 'cloud point' method, and tie-line compositions were obtained by density measurements. The values of selectivity and distribution coefficient, derived from the tie-line data, were used to decide if this ionic liquid can be used as potential solvent for the separation of benzene from aliphatic hydrocarbons using liquid extraction. These results were analyzed and compared to those previously reported for the systems {hexane + benzene + [EMpy][ESO 4 ]} and {heptane + benzene + [EMpy][ESO 4 ]}. The experimental results show that this ionic liquid is suitable for the extraction of benzene from mixtures containing octane and nonane. The consistency of tie-line data was ascertained by applying the Othmer-Tobias and Hand equations. The experimental results for the ternary systems were well correlated with the NRTL model. No literature data were found for the mixtures discussed in this paper.

  15. Zooming to the centre of the Milky Way - GigaGalaxy Zoom phase 2

    Science.gov (United States)

    2009-09-01

    The second of three images of ESO's GigaGalaxy Zoom project has just been released online. It is a new and wonderful 340-million-pixel vista of the central parts of our home galaxy as seen from ESO's Paranal Observatory with an amateur telescope. This 34 by 20-degree wide image provides us with a view as experienced by amateur astronomers around the world. However, its incredible beauty and appeal owe much to the quality of the observing site and the skills of Stéphane Guisard, the world-renowned astrophotographer, who is also an ESO engineer. This second image directly benefits from the quality of Paranal's sky, one of the best on the planet, where ESO's Very Large Telescope is located. In addition, Guisard has drawn on his professional expertise as an optical engineer specialising in telescopes, a rare combination in the world of astrophotographers. Guisard, as head of the optical engineering team at Paranal, is responsible for ensuring that the Very Large Telescope has the best optical performance possible. To create this stunning, true-colour mosaic of the Galactic Centre region, Guisard assembled about 1200 individual images, totalling more than 200 hours of exposure time, collected over 29 nights, during Guisard's free time, while working during the day at Paranal [1]. The image shows the region spanning the sky from the constellation of Sagittarius (the Archer) to Scorpius (the Scorpion). The very colourful Rho Ophiuchi and Antares region is a prominent feature to the right, although much darker areas, such as the Pipe and Snake nebulae also stand out. The dusty lane of our Milky Way runs obliquely through the image, dotted with remarkable bright, reddish nebulae, such as the Lagoon and the Trifid Nebulae, as well as NGC 6357 and NGC 6334. This dark lane also hosts the very centre of our Galaxy, where a supermassive black hole is lurking. "The area I have depicted in this image is an incredibly rich region of the sky, and the one I find most beautiful

  16. Synthesis and properties of a novel bio-based polymer from modified soybean oil

    Science.gov (United States)

    Li, Y. T.; Yang, L. T.; Zhang, H.; Tang, Z. J.

    2017-02-01

    Maleated acrylated epoxidized soybean oil (MAESO) was prepared by acrylated epoxidized soybean oil (AESO) and maleic anhydride. AESO were obtained by the reaction of epoxidized soybean oil (ESO) with acrylic acid as the ring-opening reagent. The polymer was prepared by MAESO react with styrene. The structures of the products were studied by Fourier transformation infrared spectrometer (FT-IR), and were consistent with the theoretical structures. Swelling experiment indicated that the crosslinking degree increased with increasing epoxy value of ESO. Thermal properties was tested by thermo-gravimetric analysis (TG) and differential scanning calorimetry analysis (DSC), indicating that glass transition temperature (Tg) of the polymer increased with increasing epoxy value of ESO, and thermal stability of polymer have a good correlation with the crosslinking degree. Mechanical properties analysis presented that tensile strength and impact strength affected by epoxy value of ESO. With the increase of epoxy value, the tensile strength increase, while the impact strength decrease. The property of the polymer ranged from elastomer to plastic character depended on the functionality of the ESO.

  17. VizieR Online Data Catalog: Circumstellar debris discs (Maldonado+, 2015)

    Science.gov (United States)

    Maldonado, J.; Eiroa, C.; Villaver, E.; Montesinos, B.; Mora, A.

    2015-09-01

    The high-resolution spectra used in this work come from several spectrographs and telescopes and have already been used in some of our previous works (Maldonado et al., 2010, Cat. J/A+A/521/A12, 2012, Cat. J/A+A/541/A40, 2013, Cat. J/A+A/554/A84; Martinez-Arnaiz et al., 2010, Cat. J/A+A/520/A79), which can be consulted for details concerning the observing runs and the reduction procedure. Summarising, the data were taken with the following instruments: i) FOCES at the 2.2-m telescope of the Calar Alto observatory (CAHA, Almeria, Spain); ii) SARG at the Telescopio Nazionale Galileo (TNG, 3.58m), La Palma (Canary Islands, Spain); iii) FIES at the Nordic Optical Telescope (NOT, 2.56m), La Palma; and iv) HERMES at the Mercator telescope (1.2m), also in La Palma. We used additional spectra from the public library "S4N" (Allende Prieto et al., 2004, Cat. J/A+A/420/183), which contains spectra taken with the 2d coude spectrograph at McDonald Observatory and the FEROS instrument at the ESO 1.52m telescope in La Silla; from the ESO/ST-ECF Science Archive Facility (http://archive.eso.org/cms/); and from the pipeline processed FEROS and HARPS data archive (http://archive.eso.org/wdb/wdb/eso/repro/form). (2 data files).

  18. CURADO NO-ISOTERMICO DE MEZCLAS EPOXI-ACEITE DE SOJA EPOXIDADO-ANHÍDRIDO

    Directory of Open Access Journals (Sweden)

    Facundo I. Altuna

    2011-01-01

    Full Text Available Se estudió la cinética de curado no-isotérmico de mezclas de diglicidil éter de bisfenol A (DGEBA con aceite de soja epoxidado (ESO utilizando anhídrido metiltetrahidroftálico (MTHPA como agente de curado y 1-metil imidazol (1MI como iniciador. El sistema ESO/MTHPA exhibió valores de entalpía de reacción menores y temperaturas de pico exotérmico mayores que el sistema DGEBA/MTHPA debido a la menor reactividad de los grupos oxirano internos del ESO. Los parámetros cinéticos de los sistemas puros DGEBA/MTHPA y ESO/MTHPA se obtuvieron a partir de los datos calorimétricos mediante un análisis de regresión multiparamétrica utilizando un modelo cinético fenomenológico. La cinética de los sistemas puros fue utilizada para predecir la de las mezclas considerando las interacciones entre DGEBA y ESO. El modelo propuesto permitió predecir adecuadamente la cinética del proceso de curado utilizando parámetros independientes de la velocidad de calentamiento y de aplicación directa en el campo de procesamiento de estos materiales.

  19. Clinical Performance of Emergency Surgical Officers in Southern Ethiopia.

    Science.gov (United States)

    Gobeze, Abera A; Kebede, Zelalem; Berhan, Yifru; Ghosh, Biku

    2016-09-01

    Serious shortage of gynecologists and surgeons for several decades leading to a three-year masters level training was initiated in 2009. However, systematic analysis was not done to assess the graduates' performance. The purpose of this study was to assess improvement in access to emergency surgical and obstetrical care services. Both quantitative and qualitative methods were employed to assess the competence of emergency surgical officers (ESOs) in their decision making and surgical skills in eight hospitals between 2012 and 2014. Anesthesia time, post-operative hospital stay and change in hemoglobin level were, among others, used as proxy indicators of their surgical skills. A total of 4075 obstetric and surgical operations was performed in the study hospitals. Of which, 93% were done on emergency base. Of the total emergency procedures, 3570(94%) were done by ESOs. Nearly two-thirds (63%) of all the emergency operations were cesarean sections, which were done by ESOs. Out of 239 uterine ruptures, hysterectomy was done for 58%. The proportion of cesarean and instrumental deliveries over the total deliveries were 13% and 0.7%, respectively. Explorative laparotomies and appendectomies were the majority of the non-obstetric emergency operations. Interviewed staff in the respective hospitals stated that ESOs' clinical decision making, surgical skill and commitment to discharge their responsibilities were in the best possible. The study showed that deployment of ESOs made the emergency surgery services accessible to the majority, and their clinical decision making and surgical skills were remarkable.

  20. Lightest exoplanet yet discovered

    Science.gov (United States)

    2009-04-01

    Well-known exoplanet researcher Michel Mayor today announced the discovery of the lightest exoplanet found so far. The planet, "e", in the famous system Gliese 581, is only about twice the mass of our Earth. The team also refined the orbit of the planet Gliese 581 d, first discovered in 2007, placing it well within the habitable zone, where liquid water oceans could exist. These amazing discoveries are the outcome of more than four years of observations using the most successful low-mass-exoplanet hunter in the world, the HARPS spectrograph attached to the 3.6-metre ESO telescope at La Silla, Chile. ESO PR Photo 15a/09 Artist's impression of Gliese 581 e ESO PR Photo 15b/09 A planet in the habitable zone ESO PR Video 15a/09 ESOcast 6 ESO PR Video 15b/09 VNR A-roll ESO PR Video 15c/09 Zoom-in on Gliese 581 e ESO PR Video 15d/09 Artist's impression of Gliese 581 e ESO PR Video 15e/09 Artist's impression of Gliese 581 d ESO PR Video 15f/09 Artist's impression of Gliese 581 system ESO PR Video 15g/09 The radial velocity method ESO PR Video 15h/09 Statement in English ESO PR Video 15i/09 Statement in French ESO PR Video 15j/09 La Silla Observatory "The holy grail of current exoplanet research is the detection of a rocky, Earth-like planet in the ‘habitable zone' -- a region around the host star with the right conditions for water to be liquid on a planet's surface", says Michel Mayor from the Geneva Observatory, who led the European team to this stunning breakthrough. Planet Gliese 581 e orbits its host star - located only 20.5 light-years away in the constellation Libra ("the Scales") -- in just 3.15 days. "With only 1.9 Earth-masses, it is the least massive exoplanet ever detected and is, very likely, a rocky planet", says co-author Xavier Bonfils from Grenoble Observatory. Being so close to its host star, the planet is not in the habitable zone. But another planet in this system appears to be. From previous observations -- also obtained with the HARPS spectrograph