WorldWideScience

Sample records for esc producing salmonella

  1. The effect of cephalosporin usage on the occurrence of ESCs producing E. coli in pig herds

    DEFF Research Database (Denmark)

    Dalhoff Andersen, Vibe; Jensen, Vibeke Frøkjær; Agersø, Yvonne

    in 19 pig herds which have had five to fourteen prescriptions of ceph. and 20 pig herds without prescribed ceph. in a previous 12 month period. The 39 herds were all integrated and represent typical Danish pig farms. The occurrence of ESCs producing E. coli in the herds were tested in a total of 9...... pooled samples per herd. A pig herd was considered positive if one or more of the nine samples contained ESCs producing E. coli. Initially, the association between usages of ceph. and occurrence of ESCs producing E. coli in the pig herds was analyzed using logistic regression, and the effect was adjusted...... of ESCs producing E. coli was estimated as risk ratio(RR). The results showed that consumption of ceph. increased the risk of occurrence of ESCs producing E. coli significantly with a RR of 5 (95% CI: 2-11). This demonstrates that ceph. usage significant affect the occurrence of ESCs resistance...

  2. IL-18-producing Salmonella inhibit tumor growth

    Science.gov (United States)

    Loeffler, Markus; Le'Negrate, Gaelle; Krajewska, Maryla; Reed, John C.

    2009-01-01

    Previous studies have shown that intravenously applied bacteria can accumulate in tumors and lead to sporadic tumor regression. Recently, systemic administration of attenuated Salmonella typhimurium was demonstrated to generate no significant side effects in humans, but also no anti-tumor responses. We report the enhanced antitumor activity in preclinical mouse cancer models of non-virulent S. typhimurium engineered to synthesize the cytokine, Interleukin-18 (IL-18). IL-18-producing bacteria (but not control bacteria) inhibit the growth of primary subcutaneous tumors as well as pulmonary metastases in immunocompetent mice challenged with syngeneic multi-drug resistant clones of murine carcinoma cell lines, without overt toxicity to normal tissues. Anti-tumor activity was associated with increased accumulation of T-lymphocytes and NK cells in tumors, and massive infiltration of granulocytes, as well as increased intra-tumoral production of several cytokines. In summary, these findings provide evidence of promising preclinical anti-tumor activity of IL-18-expressing, attenuated S. typhimurium, suggesting a novel strategy for cancer immunotherapy. PMID:18654612

  3. Emergence of a Clonal Lineage of Multidrug-Resistant ESBL-Producing Salmonella Infantis Transmitted from Broilers and Broiler Meat to Humans in Italy between 2011 and 2014.

    Science.gov (United States)

    Franco, Alessia; Leekitcharoenphon, Pimlapas; Feltrin, Fabiola; Alba, Patricia; Cordaro, Gessica; Iurescia, Manuela; Tolli, Rita; D'Incau, Mario; Staffolani, Monica; Di Giannatale, Elisabetta; Hendriksen, Rene S; Battisti, Antonio

    2015-01-01

    We report the spread of a clone of multidrug-resistant (MDR), ESBL-producing (blaCTX-M-1) Salmonella enterica subsp. enterica serovar Infantis, in the Italian broiler chicken industry and along the food-chain. This was first detected in Italy in 2011 and led to human infection in Italy in 2013-2014.A set (n = 49) of extended-spectrum cephalosporin (ESC)-resistant (R) isolates of S. Infantis (2011-2014) from humans, food-producing animals and meat thereof, were studied along with a selected set of earlier and more recent ESC-susceptible (ESC-S) isolates (n = 42, 2001-2014). They were characterized by macrorestriction-PFGE analysis and genetic environment of ESC-resistance. Isolates representative of PFGE-patterns and origin were submitted to Whole Genome Sequencing. The emerging ESC-R clone, detected mainly from broiler chickens, broiler meat and humans, showed a minimum pattern of clinical resistance to cefotaxime, tetracycline, sulfonamides, and trimethoprim, beside ciprofloxacin microbiological resistance (MIC 0.25 mg/L). All isolates of this clone harbored a conjugative megaplasmid (~ 280-320 Kb), similar to that described in ESC-susceptible S. Infantis in Israel (pESI-like) in 2014. This megaplasmid carried the ESBL gene blaCTX-M-1, and additional genes [tet(A), sul1, dfrA1 and dfrA14] mediating cefotaxime, tetracycline, sulfonamide, and trimethoprim resistance. It also contained genes conferring enhanced colonization capability, virulence (fimbriae, yersiniabactin), resistance and fitness (qacE1, mer) in the intensive-farming environment. This emerging clone of S. Infantis has been causing infections in humans, most likely through the broiler industry. Since S. Infantis is among major serovars causing human infections in Europe and is an emerging non-typhoidal Salmonella globally, further spread of this lineage in primary productions deserves quick and thorough risk-management strategies.

  4. Emergence of a Clonal Lineage of Multidrug-Resistant ESBL-Producing Salmonella Infantis Transmitted from Broilers and Broiler Meat to Humans in Italy between 2011 and 2014.

    Directory of Open Access Journals (Sweden)

    Alessia Franco

    Full Text Available We report the spread of a clone of multidrug-resistant (MDR, ESBL-producing (blaCTX-M-1 Salmonella enterica subsp. enterica serovar Infantis, in the Italian broiler chicken industry and along the food-chain. This was first detected in Italy in 2011 and led to human infection in Italy in 2013-2014.A set (n = 49 of extended-spectrum cephalosporin (ESC-resistant (R isolates of S. Infantis (2011-2014 from humans, food-producing animals and meat thereof, were studied along with a selected set of earlier and more recent ESC-susceptible (ESC-S isolates (n = 42, 2001-2014. They were characterized by macrorestriction-PFGE analysis and genetic environment of ESC-resistance. Isolates representative of PFGE-patterns and origin were submitted to Whole Genome Sequencing. The emerging ESC-R clone, detected mainly from broiler chickens, broiler meat and humans, showed a minimum pattern of clinical resistance to cefotaxime, tetracycline, sulfonamides, and trimethoprim, beside ciprofloxacin microbiological resistance (MIC 0.25 mg/L. All isolates of this clone harbored a conjugative megaplasmid (~ 280-320 Kb, similar to that described in ESC-susceptible S. Infantis in Israel (pESI-like in 2014. This megaplasmid carried the ESBL gene blaCTX-M-1, and additional genes [tet(A, sul1, dfrA1 and dfrA14] mediating cefotaxime, tetracycline, sulfonamide, and trimethoprim resistance. It also contained genes conferring enhanced colonization capability, virulence (fimbriae, yersiniabactin, resistance and fitness (qacE1, mer in the intensive-farming environment. This emerging clone of S. Infantis has been causing infections in humans, most likely through the broiler industry. Since S. Infantis is among major serovars causing human infections in Europe and is an emerging non-typhoidal Salmonella globally, further spread of this lineage in primary productions deserves quick and thorough risk-management strategies.

  5. Molecular Characterization of Extended-Spectrum Cephalosporinase-Producing Salmonella enterica Serovar Choleraesuis Isolates from Patients in Thailand and Denmark

    DEFF Research Database (Denmark)

    Sirichote, P.; Hasman, Henrik; Pulsrikarn, C.

    2010-01-01

    on susceptibility patterns, the ESC-producing isolates were more closely related than non-ESC-producing isolates. Microarray, PCR, plasmid profiling, and replicon typing revealed that the 13 ESC-producing isolates harbored either bla(CMY-2) containing incA/C or bla(CTX-M-14) containing incFIIA, inc...

  6. Imported poultry meat as a source of extended-spectrum cephalosporin-resistant CMY-2-producing Salmonella Heidelberg and Salmonella Minnesota in the European Union, 2014-2015.

    Science.gov (United States)

    Campos, Joana; Mourão, Joana; Silveira, Leonor; Saraiva, Margarida; Correia, Cristina Belo; Maçãs, Ana Paula; Peixe, Luísa; Antunes, Patrícia

    2018-01-01

    Extended-spectrum cephalosporin (ESC)-resistant Salmonella have been described at a low level in the EU, nevertheless the increasing importation of poultry meat could be an important source of epidemic strains carrying ESC resistance genes. This study evaluated ESC resistance and its genetic platform among Salmonella isolates from poultry meat products imported into Portugal as well as clonal relatedness of the isolates. All Salmonella isolates recovered from samples of fresh meat destined for import into the EU in the scope of Portuguese official border control (2014-2015) were studied. Antibiotic susceptibility and β-lactamase production was determined by disk diffusion/microdilution. Molecular studies included detection of genes encoding acquired AmpC and extended-spectrum β-lactamases, plasmid-mediated quinolone resistance and other antibiotic resistance genes by PCR/sequencing, and clonality by MLST and XbaI-PFGE. Plasmid characterisation was assessed by conjugation assays, replicon typing (PCR-PBRT/pMLST) and hybridisation experiments (I-CeuI/S1-PFGE nuclease). Isolates belonged to Salmonella Heidelberg (n = 6; ST15/eBG26) and Salmonella Minnesota (n = 1; ST548/eBG77) and presented multidrug-resistant profiles, including to ESCs and/or fluoroquinolones. All but one carried blaCMY-2, located on two epidemic plasmids, IncA/C (ST2, n = 5) or transferable IncI1 (ST12, n = 1). Salmonella Heidelberg was associated with five PFGE types, including one similar to an American epidemic clone. This study reveals imported poultry products as a source of uncommon and/or invasive ESC-resistant Salmonella strains in the EU. The increase of clinically relevant poultry-related serotypes in Europe must be taken into account in the current monitoring of antibiotic resistance trends and in re-evaluation of food regulations. Copyright © 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

  7. Effect of Chromatin Structure on the Extent and Distribution of DNA Double Strand Breaks Produced by Ionizing Radiation; Comparative Study of hESC and Differentiated Cells Lines.

    Science.gov (United States)

    Venkatesh, Priyanka; Panyutin, Irina V; Remeeva, Evgenia; Neumann, Ronald D; Panyutin, Igor G

    2016-01-02

    Chromatin structure affects the extent of DNA damage and repair. Thus, it has been shown that heterochromatin is more protective against DNA double strand breaks (DSB) formation by ionizing radiation (IR); and that DNA DSB repair may proceed differently in hetero- and euchromatin regions. Human embryonic stem cells (hESC) have a more open chromatin structure than differentiated cells. Here, we study the effect of chromatin structure in hESC on initial DSB formation and subsequent DSB repair. DSB were scored by comet assay; and DSB repair was assessed by repair foci formation via 53BP1 antibody staining. We found that in hESC, heterochromatin is confined to distinct regions, while in differentiated cells it is distributed more evenly within the nuclei. The same dose of ionizing radiation produced considerably more DSB in hESC than in differentiated derivatives, normal human fibroblasts; and one cancer cell line. At the same time, the number of DNA repair foci were not statistically different among these cells. We showed that in hESC, DNA repair foci localized almost exclusively outside the heterochromatin regions. We also noticed that exposure to ionizing radiation resulted in an increase in heterochromatin marker H3K9me3 in cancer HT1080 cells, and to a lesser extent in IMR90 normal fibroblasts, but not in hESCs. These results demonstrate the importance of chromatin conformation for DNA protection and DNA damage repair; and indicate the difference of these processes in hESC.

  8. Emergence of a Clonal Lineage of Multidrug-Resistant ESBL-Producing Salmonella Infantis Transmitted from Broilers and Broiler Meat to Humans in Italy between 2011 and 2014

    OpenAIRE

    Alessia Franco; Pimlapas Leekitcharoenphon; Fabiola Feltrin; Patricia Alba; Gessica Cordaro; Manuela Iurescia; Rita Tolli; Mario D'Incau; Monica Staffolani; Elisabetta Di Giannatale; Hendriksen, Rene S.; Antonio Battisti

    2015-01-01

    We report the spread of a clone of multidrug-resistant (MDR), ESBL-producing (bla CTX-M-1) Salmonella enterica subsp. enterica serovar Infantis, in the Italian broiler chicken industry and along the food-chain. This was first detected in Italy in 2011 and led to human infection in Italy in 2013?2014.A set (n = 49) of extended-spectrum cephalosporin (ESC)-resistant (R) isolates of S. Infantis (2011?2014) from humans, food-producing animals and meat thereof, were studied along with a selected s...

  9. Emergence of a Clonal Lineage of Multidrug-Resistant ESBL-Producing Salmonella Infantis Transmitted from Broilers and Broiler Meat to Humans in Italy between 2011 and 2014

    OpenAIRE

    Franco, Alessia; Leekitcharoenphon, Pimlapas; Feltrin, Fabiola; Alba, Patricia; Cordaro, Gessica; Iurescia, Manuela; Tolli, Rita; D'Incau, Mario; Staffolani, Monica; Di Giannatale, Elisabetta; Hendriksen, Rene S.; Battisti, Antonio

    2015-01-01

    We report the spread of a clone of multidrug-resistant (MDR), ESBL-producing (blaCTX-M-1) Salmonella enterica subsp. enterica serovar Infantis, in the Italian broiler chicken industry and along the food-chain. This was first detected in Italy in 2011 and led to human infection in Italy in 2013-2014.A set (n = 49) of extended-spectrum cephalosporin (ESC)-resistant (R) isolates of S. Infantis (2011-2014) from humans, food-producing animals and meat thereof, were studied along with a selected se...

  10. Distributions of Salmonella Subtypes Differ between Two U.S. Produce-Growing Regions

    Science.gov (United States)

    Danyluk, Michelle D.; Worobo, Randy W.; Wiedmann, Martin

    2014-01-01

    Salmonella accounts for approximately 50% of produce-associated outbreaks in the United States, several of which have been traced back to contamination in the produce production environment. To quantify Salmonella diversity and aid in identification of Salmonella contamination sources, we characterized Salmonella isolates from two geographically diverse produce-growing regions in the United States. Initially, we characterized the Salmonella serotype and subtype diversity associated with 1,677 samples collected from 33 produce farms in New York State (NYS). Among these 1,677 samples, 74 were Salmonella positive, yielding 80 unique isolates (from 147 total isolates), which represented 14 serovars and 23 different pulsed-field gel electrophoresis (PFGE) types. To explore regional Salmonella diversity associated with production environments, we collected a smaller set of samples (n = 65) from South Florida (SFL) production environments and compared the Salmonella diversity associated with these samples with the diversity found among NYS production environments. Among these 65 samples, 23 were Salmonella positive, yielding 32 unique isolates (from 81 total isolates), which represented 11 serovars and 17 different PFGE types. The most common serovars isolated in NYS were Salmonella enterica serovars Newport, Cerro, and Thompson, while common serovars isolated in SFL were Salmonella serovars Saphra and Newport and S. enterica subsp. diarizonae serovar 50:r:z. High PFGE type diversity (Simpson's diversity index, 0.90 ± 0.02) was observed among Salmonella isolates across both regions; only three PFGE types were shared between the two regions. The probability of three or fewer shared PFGE types was Salmonella isolates were considerably different between the two sampled regions. These findings suggest the potential for PFGE-based source tracking of Salmonella in production environments. PMID:24747908

  11. Salmonella and produce: survival in the plant environment and implications in food safety.

    Science.gov (United States)

    Fatica, Marianne K; Schneider, Keith R

    2011-01-01

    There has been a continuous rise in the number of produce-based foodborne outbreaks in the recent decades despite the perception that foodborne diseases were primarily linked to animal-based products. The Centers for Disease Control and Prevention (CDC) estimates that 95% of Salmonella-based infections originate from foodborne sources, with multiple produce-based salmonellosis outbreaks occurring since 1990. The contamination of produce in both the pre-harvest and post-harvest produce environments is challenging to eliminate since produce is consumed as a raw, fresh commodity. Salmonella spp. contamination is possible through contact with the produce in the field as well as in the processing facility. The field contamination of produce infers the ability of Salmonella spp. to survive on the plant surface. The fitness of Salmonella spp. in the plant habitat is limited as opposed to naturally plant-associated bacteria, but survival is possible. The use of intensive farming practices, globalization of food products, high demand for convenience food products, and increased foodborne disease surveillance also have unknown ramifications in the ascending trends of produce-based outbreaks. A better understanding of the ecology of Salmonella spp. in the plant environment as well as the processing, food handling, and surveillance factors affecting the incidence of foodborne outbreaks will provide a comprehensive view of the etiology and epidemiology of produce-associated foodborne outbreaks. An understanding of the outbreaks and the factors facilitating produce contamination will allow for the development of intervention procedures and strategies to reduce the risk of produce contamination by Salmonella spp.

  12. Prevalence of extended-spectrum cephalosporinase (ESC)-producing Escherichia coli in Danish slaughter pigs and retail meat identified by selective enrichment and association with cephalosporin usage

    DEFF Research Database (Denmark)

    Agersø, Yvonne; Aarestrup, Frank Møller; Pedersen, Karl

    2012-01-01

    (n = 786) were collected at slaughter, and 866 meat samples [Danish: pork (153), broiler meat (121) and beef (142); and imported: pork (173), broiler meat (193) and beef (84)] were randomly collected in retail stores and outlets. E. coli was isolated after enrichment in MacConkey broth......OBJECTIVES: To investigate the prevalence of extended-spectrum cephalosporinase (ESC)-producing Escherichia coli in pigs at slaughter and retail meat, and possible associations with the consumption of third- and fourth-generation cephalosporins. METHODS: During 2009, faecal samples from Danish pigs...

  13. Risk assessment of Salmonella in Danish meatballs produced in the catering sector

    DEFF Research Database (Denmark)

    Møller, Cleide Oliveira de Almeida; Nauta, Maarten; Schaffner, Donald W.

    2015-01-01

    observational data and models that were specific for Salmonella spp. in meatballs produced in the catering sector. Danish meatballs are often pan-fried followed by baking in an oven before consumption, in order to reach the core temperature of 75 degrees C recommended by the Danish Food Safety Authority...... of meatballs to core temperatures higher than 70 degrees C, and subsequent holding at room temperatures lower than 20 degrees C, for no longer than 3.5 h, were very effective in Salmonella control. The current Danish Food Safety Authority recommendation of cooking to an internal temperature of 75 degrees C...... is conservative, at least with respect to Salmonella risk. Survival and growth of Salmonella during cooling of meatballs not heat treated in oven had a significant impact on the risk estimates, and therefore, cooling should be considered a critical step during meatball processing. (c) 2014 Published by Elsevier B.V....

  14. Improved cytotoxic effects of Salmonella-producing cytosine deaminase in tumour cells.

    Science.gov (United States)

    Mesa-Pereira, Beatriz; Medina, Carlos; Camacho, Eva María; Flores, Amando; Santero, Eduardo

    2015-01-01

    In order to increase the cytotoxic activity of a Salmonella strain carrying a salicylate-inducible expression system that controls cytosine deaminase production, we have modified both, the vector and the producer bacterium. First, the translation rates of the expression module containing the Escherichia coli codA gene cloned under the control of the Pm promoter have been improved by using the T7 phage gene 10 ribosome binding site sequence and replacing the original GUG start codon by AUG. Second, to increase the time span in which cytosine deaminase may be produced by the bacteria in the presence of 5-fluorocytosine, a 5-fluorouracyl resistant Salmonella strain has been constructed by deleting its upp gene sequence. This new Salmonella strain shows increased cytosine deaminase activity and, after infecting tumour cell cultures, increased cytotoxic and bystander effects under standard induction conditions. In addition, we have generated a purD mutation in the producer strain to control its intracellular proliferation by the presence of adenine and avoid the intrinsic Salmonella cell death induction. This strategy allows the analysis and comparison of the cytotoxic effects of cytosine deaminase produced by different Salmonella strains in tumour cell cultures. © 2014 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

  15. High occurrence of extended-spectrum β-lactamase-producing Salmonella in broiler carcasses from poultry slaughterhouses in South Korea.

    Science.gov (United States)

    Chon, Jung-Whan; Jung, Hae-In; Kuk, Min; Kim, Young-Ji; Seo, Kun-Ho; Kim, Soo-Ki

    2015-03-01

    The emergence of antibiotic-resistant foodborne Salmonella has become a major public health problem. Consumption of undercooked poultry contaminated with Salmonella can induce food poisoning in humans. In this study, we investigated the occurrence and antibiotic resistance patterns of Salmonella spp. isolated from 120 chicken carcasses produced in 6 poultry slaughterhouses in South Korea. A total of 11 samples (9.2%) were found contaminated with Salmonella: 5 isolates were serotyped as Salmonella Bellevue strain (slaughterhouse C) and 6 isolates were serotyped as Salmonella Enteritidis strain (slaughterhouse E). Salmonella Bellevue isolates were resistant to five antibiotics (ampicillin, chloramphenicol, nalidixic acid, tetracycline, and trimethoprim/sulfamethoxazole), while Salmonella Enteritidis isolates were resistant to nine antibiotics (ampicillin, cefotaxime, ceftazidime, cefazolin, cephalothin, amikacin, nalidixic acid, streptomycin, and tetracycline). All cephalosporin-resistant Salmonella Enteritidis isolates exhibited the extended-spectrum β-lactamase (ESBL) phenotype and carried the gene encoding CTX-M-15, the most prevalent ESBL enzyme worldwide. Based on molecular subtyping performed using the automated rep-polymerase chain reaction (PCR) system (DiversiLab), the isolates showing ≥ 95 similarity in their rep-PCR banding patterns were classified into 5 pulsotypes. Given that cephalosporins are the drugs of choice for invasive Salmonella infections, the high incidence of ESBL-producing strains in chicken should emphasize the necessity of regular monitoring of the occurrence of antibiotic-resistant ESBL-positive Salmonella strains in poultry meat.

  16. Differences in biofilm formation of produce and poultry Salmonella enterica isolates and their persistence on spinach plants.

    Science.gov (United States)

    Patel, Jitendra; Singh, Manpreet; Macarisin, Dumitru; Sharma, Manan; Shelton, Daniel

    2013-12-01

    Spinach plants were irrigated biweekly with water containing 2.1 log CFU Salmonella/100 ml water (the maximum Escherichia coli MPN recommended by the Leafy Greens Marketing Agreement; LGMA), or 4.1 CFU Salmonella/100 ml water to determine Salmonella persistence on spinach leaves. Green Fluorescent protein expressing Salmonella were undetectable by most-probable number (MPN) at 24 h and 7 days following each irrigation event. This study indicates that Salmonella are unlikely to persist on spinach leaves when irrigation water is contaminated at a level below the LGMA standards. In a parallel study, persistence of Salmonella isolated from poultry or produce was compared following biweekly irrigation of spinach plants with water containing 6 log CFU Salmonella/100 ml. Produce Salmonella isolates formed greater biofilms on polystyrene, polycarbonate and stainless steel surfaces and persisted at significantly higher numbers on spinach leaves than those Salmonella from poultry origin during 35 days study. Poultry Salmonella isolates were undetectable (spinach plants 7 days following each irrigation event when assayed by direct plating. This study indicates that Salmonella persistence on spinach leaves is affected by the source of contamination and the biofilm forming ability of the strain. Published by Elsevier Ltd.

  17. Efficacy of myrtle oil against Salmonella Typhimurium on fresh produce.

    Science.gov (United States)

    Gündüz, Gülten Tiryaki; Gönül, Sahika Aktuğ; Karapinar, Mehmet

    2009-03-31

    The antimicrobial activity of myrtle leaves (Myrtus communis) oil was tested against the nalidixic acid resistant strain of Salmonella Typhimurium ATCC 13311. An inoculum (100 microl, ca.10(8) cfu/ml) was deposited on the skin of whole tomatoes and 10 g of shredded iceberg lettuce, dried for 2 h at 22 degrees C and held for 22 h at 4 degrees C before treatments. Inoculated iceberg lettuce (3.51-3.99 log cfu/g) and tomatoes (3.47-4.86 log cfu/tomato) were treated with three different washing procedures for 5, 10, 15 and 20 min; washing with sterile distilled water (control), washing with three different concentrations of myrtle leaves oil and the last treatment was a combination of washing with myrtle leaves oil and then rinsing in sterile distilled water for 1 min. Washing with myrtle leaves oil with or without rinsing procedures caused significant reduction in S. Typhimurium population compared with the control after treatment for four different times (p0.05). The maximum logarithmic reductions of 1.66 cfu/g-1.89 cfu/tomato were respectively obtained on iceberg lettuce and tomatoes treated with 1000 ppm myrtle leaves oil without any rinsing treatment. The results suggest that the use of myrtle leaves oil is an innovative and useful tool as an alternative to the use of chlorine or other synthetic disinfectants in fruits and vegetables, especially for organic products.

  18. Antibody-integrated and functionalized graphite-encapsulated magnetic beads, produced using ammonia gas plasma technology, for capturing Salmonella.

    Science.gov (United States)

    Sakudo, Akikazu; Chou, Han; Nagatsu, Masaaki

    2015-03-01

    Salmonella spp. is the single and most important causative agent of foodborne infections, especially involving foods such as eggs, milk and meat. To prevent infection, a reliable surveillance system is required that can quickly and sensitively detect Salmonella. Here, we describe the development of antibody-integrated magnetic beads that are functionalized by a novel strategy using ammonia gas plasma. Ammonia plasma, produced by a radio frequency (RF) power supply, was allowed to react with the surface of graphite-encapsulated magnetic beads, resulting in the introduction of amino groups. An anti-Salmonella antibody was then anchored by sulfide groups present on the protein surface to the amino groups of the magnetic beads via N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). The potential usefulness of these magnetic beads for capturing Salmonella was examined as follows. The beads were incubated with Salmonella in liquid medium and then separated from the supernatant by applying a magnetic field. After thorough washing, adsorption of Salmonella to the beads was confirmed by immunochromatography, polymerase chain reaction and a direct culture assay. Our findings indicate that the capture and concentration of Salmonella using the antibody-integrated magnetic beads was more efficient than commercial Dynabeads® anti-Salmonella, which are conventionally used for concentrating Salmonella from liquid cultures. We believe this novel bead technology will contribute to the enhanced detection of Salmonella. Copyright © 2015 Elsevier Ltd. All rights reserved.

  19. Selected lactic acid-producing bacterial isolates with the capacity to reduce Salmonella translocation and virulence gene expression in chickens.

    Science.gov (United States)

    Yang, Xiaojian; Brisbin, Jennifer; Yu, Hai; Wang, Qi; Yin, Fugui; Zhang, Yonggang; Sabour, Parviz; Sharif, Shayan; Gong, Joshua

    2014-01-01

    Probiotics have been used to control Salmonella colonization/infection in chickens. Yet the mechanisms of probiotic effects are not fully understood. This study has characterized our previously-selected lactic acid-producing bacterial (LAB) isolates for controlling Salmonella infection in chickens, particularly the mechanism underlying the control. In vitro studies were conducted to characterize 14 LAB isolates for their tolerance to low pH (2.0) and high bile salt (0.3-1.5%) and susceptibility to antibiotics. Three chicken infection trials were subsequently carried out to evaluate four of the isolates for reducing the burden of Salmonella enterica serovar Typhimurium in the broiler cecum. Chicks were gavaged with LAB cultures (10(6-7) CFU/chick) or phosphate-buffered saline (PBS) at 1 day of age followed by Salmonella challenge (10(4) CFU/chick) next day. Samples of cecal digesta, spleen, and liver were examined for Salmonella counts on days 1, 3, or 4 post-challenge. Salmonella in the cecum from Trial 3 was also assessed for the expression of ten virulence genes located in its pathogenicity island-1 (SPI-1). These genes play a role in Salmonella intestinal invasion. Tested LAB isolates (individuals or mixed cultures) were unable to lower Salmonella burden in the chicken cecum, but able to attenuate Salmonella infection in the spleen and liver. The LAB treatments also reduced almost all SPI-1 virulence gene expression (9 out of 10) in the chicken cecum, particularly at the low dose. In vitro treatment with the extracellular culture fluid from a LAB culture also down-regulated most SPI-1 virulence gene expression. The possible correlation between attenuation of Salmonella infection in the chicken spleen and liver and reduction of Salmonella SPI-1 virulence gene expression in the chicken cecum by LAB isolates is a new observation. Suppression of Salmonella virulence gene expression in vivo can be one of the strategies for controlling Salmonella infection in chickens.

  20. Risk assessment of Salmonella in Danish meatballs produced in the catering sector.

    Science.gov (United States)

    Møller, Cleide O de A; Nauta, Maarten J; Schaffner, Donald W; Dalgaard, Paw; Christensen, Bjarke B; Hansen, Tina B

    2015-03-02

    A modular process risk model approach was used to assess health risks associated with Salmonella spp. after consumption of the Danish meatball product (frikadeller) produced with fresh pork in a catering unit. Meatball production and consumption were described as a series of processes (modules), starting from 1.3kg meat pieces through conversion to 70g meatballs, followed by a dose response model to assess the risk of illness from consumption of these meatballs. Changes in bacterial prevalence, concentration, and unit size were modelled within each module. The risk assessment was built using observational data and models that were specific for Salmonella spp. in meatballs produced in the catering sector. Danish meatballs are often pan-fried followed by baking in an oven before consumption, in order to reach the core temperature of 75°C recommended by the Danish Food Safety Authority. However, in practice this terminal heat treatment in the oven may be accidentally omitted. Eleven production scenarios were evaluated with the model, to test the impact of heat treatments and cooling rates at different room temperatures. The risk estimates revealed that a process comprising heat treatment of meatballs to core temperatures higher than 70°C, and subsequent holding at room temperatures lower than 20°C, for no longer than 3.5h, were very effective in Salmonella control. The current Danish Food Safety Authority recommendation of cooking to an internal temperature of 75°C is conservative, at least with respect to Salmonella risk. Survival and growth of Salmonella during cooling of meatballs not heat treated in oven had a significant impact on the risk estimates, and therefore, cooling should be considered a critical step during meatball processing. Copyright © 2014. Published by Elsevier B.V.

  1. Evaluation of loop-mediated isothermal amplification for the rapid, reliable, and robust detection of Salmonella in produce.

    Science.gov (United States)

    Yang, Qianru; Wang, Fei; Jones, Kelly L; Meng, Jianghong; Prinyawiwatkul, Witoon; Ge, Beilei

    2015-04-01

    Rapid, reliable, and robust detection of Salmonella in produce remains a challenge. In this study, loop-mediated isothermal amplification (LAMP) was comprehensively evaluated against real-time quantitative PCR (qPCR) for detecting diverse Salmonella serovars in various produce items (cantaloupe, pepper, and several varieties of lettuce, sprouts, and tomato). To mimic real-world contamination events, produce samples were surface-inoculated with low concentrations (1.1-2.9 CFU/25 g) of individual Salmonella strains representing ten serovars and tested after aging at 4 °C for 48 h. Four DNA extraction methods were also compared using produce enrichment broths. False-positive or false-negative results were not observed among 178 strains (151 Salmonella and 27 non-Salmonella) used to evaluate assay specificity. The detection limits for LAMP were 1.8-4 CFU per reaction in pure culture and 10(4)-10(6) CFU per 25 g (i.e., 10(2)-10(4) CFU per g) in produce without enrichment, comparable to those obtained by qPCR. After 6-8 h of enrichment, both LAMP and qPCR consistently detected these low concentrations of Salmonella of diverse serovars in all produce items except sprouts. The PrepMan Ultra sample preparation reagent yielded the best results among the four DNA extraction methods. Upon further validation, LAMP may be a valuable tool for routine Salmonella testing in produce. The difficulty of detecting Salmonella in sprouts, whether using LAMP or qPCR, warrants further study. Published by Elsevier Ltd.

  2. Dynamic status of REST in the mouse ESC pluripotency network.

    Directory of Open Access Journals (Sweden)

    Sanjay K Singh

    Full Text Available BACKGROUND: REST is abundantly expressed in mouse embryonic stem cells (ESCs. Many genome-wide analyses have found REST to be an integral part of the ESC pluripotency network. However, experimental systems have produced contradictory findings: (1 REST is required for the maintenance of ESC pluripotency and loss of REST causes increased expression of differentiation markers, (2 REST is not required for the maintenance of ESC pluripotency and loss of REST does not change expression of differentiation markers, and (3 REST is not required for the maintenance of ESC pluripotency but loss of REST causes decreased expression of differentiation markers. These reports highlight gaps in our knowledge of the ESC network. METHODS: Employing biochemical and genome-wide analyses of various culture conditions and ESC lines, we have attempted to resolve some of the discrepancies in the literature. RESULTS: We show that Rest+/- and Rest-/- AB-1 mutant ESCs, which did not exhibit a role of REST in ESC pluripotency when cultured in the presence of feeder cells, did show impaired self-renewal when compared with the parental cells under feeder-free culture conditions, but only in early passage cells. In late passage cells, both Rest+/- and Rest-/- AB-1 ESCs restored pluripotency, suggesting a passage and culture condition-dependent response. Genome-wide analysis followed by biochemical validation supported this response and further indicated that the restoration of pluripotency was associated by increased expression of the ESC pluripotency factors. E14Tg2a.4 ESCs with REST-knockdown, which earlier showed a REST-dependent pluripotency when cultured under feeder-free conditions, as well as Rest-/- AB-1 ESCs, showed no REST-dependent pluripotency when cultured in the presence of either feeder cells or laminin, indicating that extracellular matrix components can rescue REST's role in ESC pluripotency. CONCLUSIONS: REST regulates ESC pluripotency in culture condition

  3. Prevalence, Distribution, and Diversity of Salmonella enterica in a Major Produce Region of California▿†

    Science.gov (United States)

    Gorski, Lisa; Parker, Craig T.; Liang, Anita; Cooley, Michael B.; Jay-Russell, Michele T.; Gordus, Andrew G.; Atwill, E. Robert; Mandrell, Robert E.

    2011-01-01

    A survey was initiated to determine the prevalence of Salmonella enterica in the environment in and around Monterey County, CA, a major agriculture region of the United States. Trypticase soy broth enrichment cultures of samples of soil/sediment (n = 617), water (n = 252), wildlife (n = 476), cattle feces (n = 795), and preharvest lettuce and spinach (n = 261) tested originally for the presence of pathogenic Escherichia coli were kept in frozen storage and later used to test for the presence of S. enterica. A multipathogen oligonucleotide microarray was employed to identify a subset of samples that might contain Salmonella in order to test various culture methods to survey a larger number of samples. Fifty-five of 2,401 (2.3%) samples yielded Salmonella, representing samples obtained from 20 different locations in Monterey and San Benito Counties. Water had the highest percentage of positives (7.1%) among sample types. Wildlife yielded 20 positive samples, the highest number among sample types, with positive samples from birds (n = 105), coyotes (n = 40), deer (n = 104), elk (n = 39), wild pig (n = 41), and skunk (n = 13). Only 16 (2.6%) of the soil/sediment samples tested positive, and none of the produce samples had detectable Salmonella. Sixteen different serotypes were identified among the isolates, including S. enterica serotypes Give, Typhimurium, Montevideo, and Infantis. Fifty-four strains were sensitive to 12 tested antibiotics; one S. Montevideo strain was resistant to streptomycin and gentamicin. Pulsed-field gel electrophoresis (PFGE) analysis of the isolates revealed over 40 different pulsotypes. Several strains were isolated from water, wildlife, or soil over a period of several months, suggesting that they were persistent in this environment. PMID:21378057

  4. Factors affecting compost tea as a potential source of Escherichia coli and Salmonella on fresh produce.

    Science.gov (United States)

    Ingram, D T; Millner, P D

    2007-04-01

    Compost tea (CT) is an unheated on-farm infusion of compost used as a spray or soil drench to promote plant growth and control foliar and root diseases. Because food safety involves all aspects from farm to fork, CT should meet basic microbiological criteria for water quality. This report describes the effects of two CT production processes, aerated and nonaerated, on growth and survival of foodborne pathogens and fecal coliforms. Seven commercially available nutrients used to supplement CT were tested individually and in combination for their effects on the growth of Escherichia coli and Salmonella. Compost containing 10(1) to 10(3) CFU/g initial concentrations of E. coli O157:H7 and Salmonella Enteritidis were used to assess growth and survival responses to aerated CT (36-h preparations) and nonaerated CT (8.5-day preparations). Pathogen and fecal coliform populations were undetectable by 8.5 days in nonaerated CT without nutrient supplements. E. coli O157:H7 decreased to below detection levels in aerated CT at 36 h without the use of supplements. In contrast, the addition of commercially formulated mixtures or combinations of nutrient supplements resulted in growth of E. coli O157: H7, Salmonella, and fecal coliforms by 1 to 4 log CFU/g in both aerated and nonaerated CT. When nutrient supplements were added, aerated CT sustained higher concentrations of E. coli O157:H7, Salmonella, and fecal coliforms than did nonaerated CT. Thus, addition of supplements supports growth of human pathogens from very low initial concentrations in both aerated and nonaerated CT and should be avoided when CT is used on fresh produce.

  5. Selected Lactic Acid-Producing Bacterial Isolates with the Capacity to Reduce Salmonella Translocation and Virulence Gene Expression in Chickens

    OpenAIRE

    Xiaojian Yang; Jennifer Brisbin; Hai Yu; Qi Wang; Fugui Yin; Yonggang Zhang; Parviz Sabour; Shayan Sharif; Joshua Gong

    2014-01-01

    BACKGROUND: Probiotics have been used to control Salmonella colonization/infection in chickens. Yet the mechanisms of probiotic effects are not fully understood. This study has characterized our previously-selected lactic acid-producing bacterial (LAB) isolates for controlling Salmonella infection in chickens, particularly the mechanism underlying the control. METHODOLOGY/PRINCIPAL FINDINGS: In vitro studies were conducted to characterize 14 LAB isolates for their tolerance to low pH (2.0) an...

  6. Selected lactic acid-producing bacterial isolates with the capacity to reduce Salmonella translocation and virulence gene expression in chickens.

    Directory of Open Access Journals (Sweden)

    Xiaojian Yang

    Full Text Available BACKGROUND: Probiotics have been used to control Salmonella colonization/infection in chickens. Yet the mechanisms of probiotic effects are not fully understood. This study has characterized our previously-selected lactic acid-producing bacterial (LAB isolates for controlling Salmonella infection in chickens, particularly the mechanism underlying the control. METHODOLOGY/PRINCIPAL FINDINGS: In vitro studies were conducted to characterize 14 LAB isolates for their tolerance to low pH (2.0 and high bile salt (0.3-1.5% and susceptibility to antibiotics. Three chicken infection trials were subsequently carried out to evaluate four of the isolates for reducing the burden of Salmonella enterica serovar Typhimurium in the broiler cecum. Chicks were gavaged with LAB cultures (10(6-7 CFU/chick or phosphate-buffered saline (PBS at 1 day of age followed by Salmonella challenge (10(4 CFU/chick next day. Samples of cecal digesta, spleen, and liver were examined for Salmonella counts on days 1, 3, or 4 post-challenge. Salmonella in the cecum from Trial 3 was also assessed for the expression of ten virulence genes located in its pathogenicity island-1 (SPI-1. These genes play a role in Salmonella intestinal invasion. Tested LAB isolates (individuals or mixed cultures were unable to lower Salmonella burden in the chicken cecum, but able to attenuate Salmonella infection in the spleen and liver. The LAB treatments also reduced almost all SPI-1 virulence gene expression (9 out of 10 in the chicken cecum, particularly at the low dose. In vitro treatment with the extracellular culture fluid from a LAB culture also down-regulated most SPI-1 virulence gene expression. CONCLUSIONS/SIGNIFICANCE: The possible correlation between attenuation of Salmonella infection in the chicken spleen and liver and reduction of Salmonella SPI-1 virulence gene expression in the chicken cecum by LAB isolates is a new observation. Suppression of Salmonella virulence gene expression in

  7. Evaluation of the ability of four ESBL-screening media to detect ESBL-producing Salmonella and Shigella.

    Science.gov (United States)

    Sturød, Kjersti; Dahle, Ulf R; Berg, Einar Sverre; Steinbakk, Martin; Wester, Astrid L

    2014-09-04

    The aim of this study was to compare the ability of four commercially available media for screening extended-spectrum beta-lactamase (ESBL) to detect and identify ESBL-producing Salmonella and Shigella in fecal samples. A total of 71 Salmonella- and 21 Shigella-isolates producing ESBL(A) and/or AmpC, were received at Norwegian Institute of Public Health between 2005 and 2012. The 92 isolates were mixed with fecal specimens and tested on four ESBL screening media; ChromID ESBL (BioMèrieux), Brilliance ESBL (Oxoid), BLSE agar (AES Chemunex) and CHROMagar ESBL (CHROMagar). The BLSE agar is a biplate consisting of two different agars. Brilliance and CHROMagar are supposed to suppress growth of AmpC-producing bacteria while ChromID and BLSE agar are intended to detect both ESBL(A) and AmpC. The total sensitivity (ESBL(A)+AmpC) with 95% confidence intervals after 24 hours of incubation were as follows: ChromID: 95% (90.4-99.6), Brilliance: 93% (87.6-98.4), BLSE agar (Drigalski): 99% (96.9-100), BLSE agar (MacConkey): 99% (96.9-100) and CHROMagar: 85% (77.5-92.5). The BLSE agar identified Salmonella and Shigella isolates as lactose-negative. The other agars based on chromogenic technology displayed Salmonella and Shigella flexneri isolates with colorless colonies (as expected). Shigella sonnei produced pink colonies, similar to the morphology described for E. coli. All four agar media were reliable in screening fecal samples for ESBL(A)-producing Salmonella and Shigella. However, only ChromID and BLSE agar gave reliable detection of AmpC-producing isolates. Identification of different bacterial species based on colony colour alone was not accurate for any of the four agars.

  8. Prevalence and antimicrobial resistance pattern of Salmonella in animal feed produced in Namibia.

    Science.gov (United States)

    Shilangale, Renatus P; Di Giannatale, Elisabetta; Chimwamurombe, Percy M; Kaaya, Godwin P

    2012-01-01

    The occurrence of Salmonella is a global challenge in the public health and food production sectors. Our study investigated the prevalence, serovar and antimicrobial susceptibility of strains of Salmonella serovars isolated from animal feed (meat-and-bone and blood meal) samples from two commercial abattoirs in Namibia. A total of 650 samples (n=650) were examined for the presence of Salmonella. Results showed that 10.9% (n=71) were positive for Salmonella. Of the Salmonella serovars isolated, S. Chester was the most commonly isolated serovar (19.7%), followed by S. Schwarzengrund at 12.7%. From the Salmonella isolates, 19.7% (n=14) were resistant to one or more of the antimicrobials (nalidixic acid, trimethoprim-sulfamethoxazole, sulfisoxazole, streptomycin and/or tetracycline), whereas 80.3% (n=57) were susceptible to all 16 antimicrobials tested. Resistance to sulfisoxazole and the trimethroprimsuflamethoxazole combination were the most common. The resistant isolates belonged to ten different Salmonella serovars. The susceptibility of most of the Salmonella isolated to the antimicrobials tested indicates that anti-microbial resistance is not as common and extensive in Namibia as has been reported in many other countries. It also appears that there is a range of antimicrobials available that are effective in managing Salmonella infections in Namibia. However, there is some evidence that resistance is developing and this will need further monitoring to ensure it does not become a problem.

  9. blaCTX-M-I group extended spectrum beta lactamase-producing Salmonella typhi from hospitalized patients in Lagos, Nigeria

    Directory of Open Access Journals (Sweden)

    Akinyemi KO

    2015-05-01

    Full Text Available Kabiru O Akinyemi,1 Bamidele A Iwalokun,2 Olajide O Alafe,1 Sulaiman A Mudashiru,1 Christopher Fakorede,11Department of Microbiology, Lagos State University, Ojo, Lagos, Nigeria; 2Biochemistry and Nutrition Division, Nigerian Institute of Medical Research, Yaba, Lagos, NigeriaPurpose: The global spread of blaCTX-M-I extended-spectrum beta-lactamase (ESBL-producing Salmonella spp. remains a major threat to treatment and control. Evidence of emergence and spread of this marker are lacking in Nigeria. This study investigated blaCTX-M-I ESBL production among Salmonella isolates from hospitalized patients.Methods: Patients (158 total made up of two groups were evaluated. Group A was composed of 135 patients with persistent pyrexia and group B was composed of 23 gastroenteritis patients and their stool samples. Samples were cultured, and isolates were identified and were subjected to antibiotic susceptibility testing by standard methods. Isolates were further screened for ESBL production, blaCTX-M-I genes and transferability by double disk synergy test, plasmid extraction, polymerase chain reaction, and conjugation experiment.Results: Thirty-five (25.9% Salmonella isolates were identified from group A, of which 74.3% were S. typhi, 22.9% were S. paratyphi and two (5.7% were invasive non-typhoidal S. enteritidis. Nine Plasmodium falciparum infections were recorded, four of which were identified as co-infections with typhoidal Salmonella. Only two (8.7% S. enteritidis samples were obtained from group B (P>0.05. A total of 24 isolates were ESBL-positive, eliciting resistance to five to seven antibiotics, and were multiple-drug resistant. ESBL production due to the blaCTX-M-I gene cluster was detected in eleven (45.8% Salmonella isolates. Nine (81.8% of the eleven blaCTX-M-I ESBL producers were S. typhi and two (18.2% isolates were S. enteritidis. Four of nine S. typhi blaCTX-M-I ESBL-producing strains harbored 23 kb self-transmissible plasmid that was co

  10. Prevalence of extended-spectrum b-lactamase-producing Salmonella on retail chicken in six provinces and two national cities in the People's Republic of China.

    Science.gov (United States)

    Wu, Haiyun; Xia, Xiaodong; Cui, Yue; Hu, Yuanyuan; Xi, Meili; Wang, Xin; Shi, Xianming; Wang, Dapeng; Meng, Jianghong; Yang, Baowei

    2013-12-01

    Prevalence of extended-spectrum β-lactamase (ESBL)-producing Salmonella in food is not well documented. This study investigated the prevalence of ESBL-producing Salmonella in 699 Salmonella isolates recovered from 1,152 retail chickens collected from six provinces and two national cities in the People's Republic of China in 2011. ESBL-producing isolates were screened by double-disk synergy test and confirmed using PCR and DNA sequencing. Of the 699 isolates tested, 60 (8.58%) were identified to be ESBL-producing Salmonella. Prevalence of ESBL-producing Salmonella was the highest in Shanghai city (17 [24.64%] of 69), followed by Shaanxi (10 [15.38%] of 65), Fujian (9 [11.69%] of 77), Guangdong (9 [7.69%] of 117), Sichuan (5 [7.25%] of 69), Beijing (6 [5.17%] of 116), Henan (4 [4.65%] of 86), and Guangxi (0 [0%] of 100) province. Significant difference (P Salmonella was found among six provinces and two cities. No significant difference (P > 0.05) in the prevalence was found between wet markets and supermarkets or between whole chickens and chopped chickens. The prevalence of ESBL-producing Salmonella differed significantly (P Salmonella varied significantly (P Salmonella serotypes: Abony (1 [33.33%] of 3), Indiana (28 [28.57%] of 98), Edinburg (6 [24.00%] of 25), Shubra (2 [20.00%] of 10), Uppsala (1 [16.67%] of 6), Thompson (8 [14.81%] of 54), Haardt (1 [12.50%] of 8), Agona (3 [9.68%] of 31), Gueuletapee (1 [6.25%] of 16), Typhimurium (4 [5.56%] of 72), Heidelberg (1 [4.55%] of 22), and Enteritidis (4 [3.17%] of 126). This study revealed that ESBL-producing Salmonella do exist in retail chicken in the People's Republic of China and that the potential risk of their presence in foods needs further exploration.

  11. Prevalence and detection of antibiotic-resistant determinant in Salmonella isolated from food-producing animals.

    Science.gov (United States)

    Igbinosa, Isoken Henrietta

    2015-01-01

    Salmonella spp. infections are considered as the most common food-borne disease globally. The contamination of food products with Salmonella has given rise to severe health and economic challenges. This study assessed the prevalence of Salmonella in the faeces of cows and goats in the Eastern Cape province of South Africa, their antibiotic resistance patterns as well as antibiotic-resistant gene determinant. Antibiotic disc was used for antibiogram profiles while polymerase chain reaction was employed for the detection of antibiotic-resistant genes. A total of 150 Salmonella were isolated from the faecal samples. Eighty two (55%) isolates were recovered from cow faeces while 68 (45%) were isolated from goat faeces. All Salmonella isolates were sensitive to ciprofloxacin (100%) while 95% were sensitive to ofloxacin. Also, a high sensitivity of 93 and 89% was observed against nalidixic acid and ofloxacin, respectively. Salmonella isolates demonstrated moderate sensitivity against cephalothin (70%), chloramphenicol (75%) and minocycline (68%) while 49% were resistant to tetracycline and erythromycin. The prevalence of the antibiotic-resistant genes in Salmonella isolates were detected as follows: integron conserved segment 28% (42/150), bla TEM gene 19.3% (29/150), blapse₁ 7.3% (11/150) and blaampC 4.7% (7/150). The results obtained in the study imply that cow and goat faeces could be potential reservoirs of Salmonella and could possibly cause infections as a result of contamination of food products. There is a need for a surveillance system to track resistance patterns of Salmonella circulating in South Africa.

  12. L-asparaginase II produced by Salmonella typhimurium inhibits T cell responses and mediates virulence.

    Science.gov (United States)

    Kullas, Amy L; McClelland, Michael; Yang, Hee-Jeong; Tam, Jason W; Torres, AnnMarie; Porwollik, Steffen; Mena, Patricio; McPhee, Joseph B; Bogomolnaya, Lydia; Andrews-Polymenis, Helene; van der Velden, Adrianus W M

    2012-12-13

    Salmonella enterica serovar Typhimurium avoids clearance by the host immune system by suppressing T cell responses; however, the mechanisms that mediate this immunosuppression remain unknown. We show that S. Typhimurium inhibit T cell responses by producing L-Asparaginase II, which catalyzes the hydrolysis of L-asparagine to aspartic acid and ammonia. L-Asparaginase II is necessary and sufficient to suppress T cell blastogenesis, cytokine production, and proliferation and to downmodulate expression of the T cell receptor. Furthermore, S. Typhimurium-induced inhibition of T cells in vitro is prevented upon addition of L-asparagine. S. Typhimurium lacking the L-Asparaginase II gene (STM3106) are unable to inhibit T cell responses and exhibit attenuated virulence in vivo. L-Asparaginases are used to treat acute lymphoblastic leukemia through mechanisms that likely involve amino acid starvation of leukemic cells, and these findings indicate that pathogens similarly use L-asparagine deprivation to limit T cell responses. Copyright © 2012 Elsevier Inc. All rights reserved.

  13. Salmonella enterica serotype Oranienburg infections associated with consumption of locally produced Tyrolean cheese.

    Science.gov (United States)

    Allerberger, F; Kreidl, P; Dierich, M.P.; Klingsbichel, E; Jenewein, D; Mader, C; Khaschabi, D; Schonbauer, M; Berghold, C

    2000-11-01

    Sixteen culture confirmed cases of enteric infection with Salmonella enterica serotype Oranienburg were detected between August 10 and September 29 1999 in Tyrol (Austria). Ten of them suffered bloody diarrhoea and six were asymptomatic carriers. Intervie

  14. Relative effectiveness of selected preenrichment media for the detection of Salmonella from leafy green produce and herbs.

    Science.gov (United States)

    Jacobson, Andrew P; Wang, Hua; Gill, Vikas S; Duvall, Robert; Arce, Gabriela; Chirtel, Stuart; Hammack, Thomas S

    2017-05-01

    Four buffered preenrichment media (BAX® System MP Media (BAX)), Universal Preenrichment Broth (UPB), modified Buffered Peptone Water (mBPW), and Buffered Peptone Water (BPW)) were compared with lactose broth (LB) in the Bacteriological Analytical Manual's (BAM) Salmonella culture method for the analysis of 9 leafy green produce and herb types. Artificially contaminated test portions were pre-enriched in each medium and the results were analyzed statistically using Fisher's Exact 2-tailed F test (p media (p > 0.05). UPB was consistently among the most effective media for recovery of Salmonella from the nine produce types; however, S. Typhimurium and S. Newport were isolated from cabbage more frequently with mBPW than with UPB (p media from all experimental trials, with leafy green produce and herbs, demonstrate that Salmonella is more effectively detected and isolated using buffered enrichments than with the currently recommended LB (p  0.05). Published by Elsevier Ltd.

  15. Characterization of extended-spectrum β-lactamases (ESBLs)-producing Salmonella in retail raw chicken carcasses.

    Science.gov (United States)

    Qiao, Jing; Zhang, Qiang; Alali, Walid Q; Wang, Jiawei; Meng, Lingyuan; Xiao, Yingping; Yang, Hua; Chen, Sheng; Cui, Shenghui; Yang, Baowei

    2017-05-02

    Extended-spectrum β-lactamases (ESBLs)-producing Salmonella is considered a serious concern to public health worldwide. However, limited information is available on ESBLs-producing Salmonella in retail chicken products in China. The objective of this study was to characterize ESBLs-producing Salmonella isolates from retail chickens in China. A total of 890 Salmonella isolates from retail chicken carcasses collected from 4 provinces were firstly screened for ESBLs-production phenotype via the double-disk synergy test method. A total of 96 (10.8%, n=890) ESBLs-producing Salmonella were identified and subjected to PFGE analysis, characterization for the presence of ESBLs encoding genes, transposons, carbapenemase and virulence genes. A total of 59 PFGE profiles were detected in these 96 isolates, among which 57.3% were found to harbor bla TEM-1 , whereas 30.2%, 24.0%, 18.8% and 7.3% were carrying bla OXA-1 , bla CTX-M-15 , bla CTX-M-3 and bla PSE-1 genes, respectively. Moreover, 42 (43.8%) isolates co-carried 2 ESBLs-producing genes, and two (2.1%) isolates co-carried 3 genes. Furthermore, 24 (25.0%) ESBLs-producing isolates carried VIM and 10 (10.4%) carried KPC encoding genes that closely associated with carbapenems resistance. Eighty-eight isolates harbored transposons ranging from 4.2% for Tn903 to 76.0% for Tn21. Out of the 88 Salmonella that harbored transposons, 25%, 22.7%, 23.9%, 10.2% and 1.1% of isolates were found to carry 2, 3, 4, 5 and 6 transposons, respectively. The minimum inhibitory concentration (MIC) values for cephalosporins (ceftriaxone, cefoperazone and cefoxitin) to ESBLs-producing isolates were from 4 to 1024μg/mL, for nalidixic acid were from 64 to 512μg/mL, for fluoroquinolones (ciprofloxacin, levofloxacin and gatifloxacin) were from 4 to 256μg/mL. Twenty-nine virulence genes were detected in the 96 ESBLs-producing isolates with 2.1% harbored spvR (lowest) and 90.6% harbored marT and steB (highest). All isolates carried at least one

  16. Eradication of Salmonella and Arizona species from turtle hatchlings produced from eggs treated on commercial turtle farms.

    Science.gov (United States)

    Siebeling, R J; Caruso, D; Neuman, S

    1984-04-01

    On commercial turtle farms more than 40% of the hatchlings excrete detectable levels of Salmonella and Arizona spp. when hatched from nonsanitized eggs incubated in sawdust or dirt-filled chambers. Over a 3-year period on 10 farms, more than 10(6) turtle eggs were treated in an attempt to hatch Salmonella-free turtles. Eggs were sanitized in disinfectant, treated by temperature- or pressure-differential dip methods in solutions containing 500 micrograms or more of gentamicin sulfate per ml, and hatched in sanitized plastic chambers free of bedding material. The Salmonella and Arizona spp. infection levels for turtles produced from treated eggs were 0 and 1.12% for years 1 and 2, respectively, whereas infection levels for hatchlings produced from nontreated eggs during these periods were 47 and 44%, respectively. During year 3, dip solutions were filtered daily, treated at 100 degrees C for 15 min on a weekly basis to free the solution of microbial contaminants and egg protein, charged with gentamicin after 10,000 to 20,000 eggs had been treated to maintain antimicrobial activity at 500 micrograms/ml or more, and maintained at pH 6.0 to preserve optimal antimicrobial activity. The implementation of these measures in year 3 resulted in an infection level of 0.15% when the tissues of 3 of 1,959 hatchlings tested were positive for Salmonella and Arizona spp., whereas the tissues of 66 (49.0%) of 135 hatchlings produced from nontreated eggs were positive.

  17. Application of water-assisted ultraviolet light in combination of chlorine and hydrogen peroxide to inactivate Salmonella on fresh produce.

    Science.gov (United States)

    Guo, Shuanghuan; Huang, Runze; Chen, Haiqiang

    2017-09-18

    With the demand for fresh produce increases in recent decades, concerns for microbiological safety of fresh produce are also raised. To identify effective ultraviolet (UV) light treatment for fresh produce decontamination, we first determined the effect of three forms of UV treatment, dry UV (samples were treated by UV directly), wet UV (samples were dipped in water briefly and then exposed to UV), and water-assisted UV (samples were treated by UV while being immersed in agitated water) on inactivation of Salmonella inoculated on tomatoes and fresh-cut lettuce. In general, the water-assisted UV treatment was found to be the most effective for both produce items. Chlorine and hydrogen peroxide were then tested to determine whether they could be used to enhance the decontamination efficacy of water-assisted UV treatment and prevent transfer of Salmonella via wash water by completely eliminating it. Neither of them significantly enhanced water-assisted UV inactivation of Salmonella on tomatoes. Chlorine significantly improved the decontamination effectiveness of the water-assisted UV treatment for baby-cut carrots and lettuce, but not for spinach. In general, the single water-assisted UV treatment and the combined treatment of water-assisted UV and chlorine were similar or more effective than the chlorine washing treatment. In most of the cases, no Salmonella was detected in the wash water when the single water-assisted UV treatment was used to decontaminate tomatoes. In a few cases when Salmonella was detected in the wash water, the populations were very low,≤2CFU/mL, and the wash water contained an extremely high level of organic load and soil level. Therefore, the single water-assisted UV treatment could potentially be used as an environmentally friendly and non-chemical alternative to chlorine washing for tomatoes after validation in industrial scale. For lettuce, spinach and baby-cut carrots, the combined treatment of water-assisted UV treatment and chlorine

  18. Assessment of Consumer Exposure to Salmonella spp., Campylobacter spp., and Shiga Toxin-Producing Escherichia coli in Meat Products at Retail in the City of Sao Paulo, Brazil.

    Science.gov (United States)

    Ristori, Christiane Asturiano; Rowlands, Ruth Estela Gravato; Martins, Cecília Geraldes; Barbosa, Maria Luisa; Dos Santos, Luis Fernando; Jakabi, Miyoko; de Melo Franco, Bernadette Dora Gombossy

    2017-08-01

    Meat products may be vehicles of bacterial pathogens to humans, and Salmonella spp., Campylobacter spp., and Shiga toxin-producing Escherichia coli (STEC) are the most relevant. The aim of this study was to generate data on prevalence of these three pathogens in 552 samples of meat products (hot dogs, pork sausages, raw ground beef, and raw chicken legs) sold at retail in the city of Sao Paulo, Brazil. Salmonella spp. was detected in 5.8% (32/552) of samples, comprising pork sausages 62.5% (20/32) and chicken legs 37.5% (12/32). The counts of Salmonella spp. were low, ranging from < 0.3 to 9.3 × 10 most probable number per gram and the most frequent serovars were Salmonella Typhimurium (28.1%), Salmonella I 4,[5],12:i:- (15.6%), Salmonella Enteritidis (12.5%), Salmonella Derby, and Salmonella Brandenburg (9.4%). Campylobacter spp. was detected in 33 samples (6.0%), comprising chicken legs (82%) and ground beef (18%). All samples were negative for STEC. These results suggest that meat products when subjected to inadequate cooking and/or cross-contamination with other products ready for consumption can lead to occurrence of outbreaks, highlighting the risks associated with them.

  19. Prevalence and clonal relationship of ESBL-producing Salmonella strains from humans and poultry in northeastern Algeria.

    Science.gov (United States)

    Djeffal, Samia; Bakour, Sofiane; Mamache, Bakir; Elgroud, Rachid; Agabou, Amir; Chabou, Selma; Hireche, Sana; Bouaziz, Omar; Rahal, Kheira; Rolain, Jean-Marc

    2017-05-15

    The aims of this study were to investigate Salmonella contamination in broiler chicken farms and slaughterhouses, to assess the antibiotic resistance profile in avian and human Salmonella isolates, and to evaluate the relationship between avian and human Extended Spectrum β-Lactamase (ESBL)-producing isolates. Salmonella was screened in different sample matrices collected at thirty-two chicken farms and five slaughterhouses. The human isolates were recovered from clinical specimens at the University Teaching Hospital of Constantine (UTH). All suspected colonies were confirmed by MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time OF light) and serotyped. Susceptibility testing against 13 antibiotics including, amoxicillin/clavulanic acid, ticarcillin, cefoxitin, cefotaxime, aztreonam, imipenem, ertapenem, gentamicin, amikacin, ciprofloxacin, colistin, trimethoprim/sulfamethoxazole and fosfomycin, was performed using the disk diffusion method on Mueller-Hinton agar. ESBL-production was screened by the double-disk synergy test and confirmed by molecular characterization using PCR (polymerase chain reaction) amplification and sequencing of ESBL encoding genes. Clonality of the avian and human strains was performed using the Multi Locus Sequencing Typing method (MLST). Forty-five isolated avian Salmonella strains and 37 human collected ones were studied. Five S. enterica serotypes were found in avian isolates (mainly Kentucky) and 9 from human ones (essentially Infantis). 51.11% and 26.6% of the avian isolates were resistant to ciprofloxacin and cefotaxime, respectively, whereas human isolates were less resistant to these antibiotics (13.5% to ciprofloxacin and 16.2% to cefotaxime). Eighteen (12 avian and 6 human) strains were found to produce ESBLs, which were identified as bla CTX-M-1 (n = 12), bla CTX-M-15 (n = 5) and bla TEM group (n = 8). Interestingly, seven of the ESBL-producing strains (5 avian and 2 human) were of the same ST (ST15) and

  20. Susceptibility profile of Salmonella against the antibacterial activity of propolis produced in two regions of Brazil

    Directory of Open Access Journals (Sweden)

    R. O. Orsi

    2005-06-01

    Full Text Available Propolis antibiotic action has been widely investigated. This assay was carried out in order to observe the in vitro antibacterial activity of propolis against Salmonella enteritidis isolated from food and Salmonella typhimurium isolated from human infections. Propolis was collected by Apis mellifera in two regions of Brazil (Mossoró, Rio Grande do Norte State; and Urubici, Santa Catarina State. Both strains survival percentage decreased with time of incubation in Ethanolic Extracts of Propolis (EEP, demonstrating bactericidal effect after 24 hours. It was also observed that EEP from Mossoró was more effective than that from Urubici. The control of the propolis solvent - 70% ethanol - was less effective than EEP, showing only a bacteriostatic effect. We can conclude that propolis shows an activity against Gram-negative bacteria that varies according to the geographical region where it was collected by bees.

  1. National surveillance of Salmonella enterica in food-producing animals in Japan

    Directory of Open Access Journals (Sweden)

    Kijima Mayumi

    2009-08-01

    Full Text Available Abstract A total of 518 fecal samples collected from 183 apparently healthy cattle, 180 pigs and 155 broilers throughout Japan in 1999 were examined to determine the prevalence and antimicrobial susceptibility of Salmonella. The isolation rates were 36.1% in broilers, 2.8% in pigs and 0.5% in cattle. S. enterica Infantis was the most frequent isolate, found in 22.6% of broiler fecal samples. Higher resistance rates were observed against oxytetracycline (82.0%, dihydrostreptomycin (77.9%, kanamycin (41.0% and trimethoprim (35.2%. Resistance rates to ampicillin, ceftiofur, bicozamycin, chloramphenicol and nalidixic acid were S. enterica Senftenberg was found in the isolates obtained from one broiler fecal sample. This is the first report of cephalosporin-resistant Salmonella directly isolated from food animal in Japan.

  2. Modification of the BAX System PCR assay for detecting Salmonella in beef, produce, and soy protein isolate. Performance Tested Method 100201.

    Science.gov (United States)

    Peng, Linda X; Wallace, Morgan; Andaloro, Bridget; Fallon, Dawn; Fleck, Lois; Delduco, Dan; Tice, George

    2011-01-01

    The BAX System PCR assay for Salmonella detection in foods was previously validated as AOAC Research Institute (RI) Performance Tested Method (PTM) 100201. New studies were conducted on beef and produce using the same media and protocol currently approved for the BAX System PCR assay for E. coli O157:H7 multiplex (MP). Additionally, soy protein isolate was tested for matrix extension using the U.S. Food and Drug Administration-Bacteriological Analytical Manual (FDA-BAM) enrichment protocols. The studies compared the BAX System method to the U.S. Department of Agriculture culture method for detecting Salmonella in beef and the FDA-BAM culture method for detecting Salmonella in produce and soy protein isolate. Method comparison studies on low-level inoculates showed that the BAX System assay for Salmonella performed as well as or better than the reference method for detecting Salmonella in beef and produce in 8-24 h enrichment when the BAX System E. coli O157:H7 MP media was used, and soy protein isolate in 20 h enrichment with lactose broth followed by 3 h regrowth in brain heart infusion broth. An inclusivity panel of 104 Salmonella strains with diverse serotypes was tested by the BAX System using the proprietary BAX System media and returned all positive results. Ruggedness factors involved in the enrichment phase were also evaluated by testing outside the specified parameters, and none of the factors examined affected the performance of the assay.

  3. Incidence of Nontyphoidal Salmonella in Food-Producing Animals, Animal Feed, and the Associated Environment in South Africa, 2012-2014.

    Science.gov (United States)

    Magwedere, Kudakwashe; Rauff, Dionne; De Klerk, Grietjie; Keddy, Karen H; Dziva, Francis

    2015-11-01

    Nontyphoidal salmonellosis continues to pose a global threat to human health, primarily by causing food-borne illnesses, and food-producing animals are the principal reservoirs of many pathogenic serovars. To identify key control points and generate information that may enable future estimation of the transmission routes between the environment, animals, and humans, we examined data on Salmonella isolates in South Africa. Samples were obtained from livestock and poultry on farms, meat at abattoirs, raw materials at feed mills, animal feed, and environmental sources (eg, poultry houses, abattoirs, feed mills, water) from 2012 to 2014 in compliance with each establishment's protocols conforming to International Organization for Standardization (ISO) (ISO/TS 17728, ISO 18593:2004 and ISO 17604:2003) standards. Isolation and serotyping of Salmonella were performed according to the scope of accreditation of the respective laboratories conforming to ISO/IEC 17025:2005 standard techniques. Salmonella was isolated from 9031 of 180 298 (5.0%) samples, and these isolates were distributed among 188 different serovars. Salmonella Enteritidis was the most frequent isolate, with 1944 of 180 298 (21.5%) originating from poultry on farms, poultry meat, and poultry houses, followed by Salmonella Havana, with 677 of 180 298 (7.5%), mostly from environmental samples. Serovars that are uncommonly associated with human disease (Salmonella Idikan, Salmonella Salford, and Salmonella Brancaster) were isolated at higher frequencies than Salmonella Typhimurium, a common cause of human illness. Environmental samples accounted for 3869 of 9031 (42.8%) samples positive for Salmonella. We describe the frequent isolation of Salmonella of a wide variety of serovars, from an array of animal feeds, food animals, and food animal environment. As prevention of human salmonellosis requires the effective control of Salmonella in food animals, these data can be used to facilitate Salmonella control in

  4. Audouin's gull, a potential vehicle of an extended spectrum β-lactamase producing Salmonella Agona.

    Science.gov (United States)

    Antilles, Noelia; Garcia-Migura, Lourdes; Joensen, Katrine Grimstrup; Leekitcharoenphon, Pimlapas; Aarestrup, Frank M; Cerdà-Cuéllar, Marta; Hendriksen, Rene S

    2015-01-01

    The genome of a multidrug-resistant Salmonella Agona isolated from Larus audouinii (Audouin's gull) in Spain was examined. The isolate showed high levels of resistance to different antimicrobials, including third generation cephalosporins and fluoroquinolones, which is a public health concern as those being used to treat severe salmonellosis in humans. Whole genome sequencing revealed the strain being multilocus sequence type ST13, and eight resistance genes (aadA2, aadB, blaCTX-M-9,blaDHA-1, qnrA1, tetA, sul1 and dfrA16) belonging to seven antimicrobial classes were confirmed, as well as the presence of two plasmids. Migratory Audouin's gulls have the ability to cover long distances during annual movements. Therefore, they have the potential to disseminate multidrug-resistant Salmonella and resistance genes in the environment and over great geographic distances, contributing to the global dissemination of resistance genes. © FEMS 2014. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  5. Arteritis with left carotid artery thrombosis produced by Salmonella enteritides. Study with CT, MR and angiography with digital subtraction. Arteritis con trombosis carotidea izquierda por Salmonella enteritidis. Estudio con TC, RM y angiografia con sustraccion digital

    Energy Technology Data Exchange (ETDEWEB)

    Iribarren Marin, M.A.; Fernandez Cruz, J.; Serrano Gotarredona, P.; Reyes Dominguez, M.J. (Hospital Universitario Virgen del Rocio, Sevilla (Spain))

    1994-01-01

    We present a case of suppurative arteritis of left common carotid artery produced by Salmonella enteritides in a 66-year-old man. We show the findings obtained by CT, MR and selective arteriography of the supra-aortic branches. We review this uncommon disorder. (Author)

  6. Infiltration of matrix-non-producers weakens the Salmonella biofilm and impairs its antimicrobial tolerance and pathogenicity

    Directory of Open Access Journals (Sweden)

    Srinandan eChakravarthy

    2015-12-01

    Full Text Available Bacterial biofilms display a collective lifestyle, wherein the cells secrete extracellular polymeric substances (EPS that helps in adhesion, aggregation, stability, and to protect the bacteria from antimicrobials. We asked whether the EPS could act as a public good for the biofilm and observed that infiltration of cells that do not produce matrix components weakened the biofilm of Salmonella enterica serovar Typhimurium. EPS production was costly for the producing cells, as indicated by a significant reduction in the fitness of wild type (WT cells during competitive planktonic growth relative to the non-producers. Infiltration frequency of non-producers in the biofilm showed a concomitant decrease in overall productivity. It was apparent in the confocal images that the non-producing cells benefit from the EPS produced by the Wild Type (WT to stay in the biofilm. The biofilm containing non-producing cells were more significantly susceptible to sodium hypochlorite and ciprofloxacin treatment than the WT biofilm. Biofilm infiltrated with non-producers delayed the pathogenesis, as tested in a murine model. The cell types were spatially assorted, with non-producers being edged out in the biofilm. However, cellulose was found to act as a barrier to keep the non-producers away from the WT microcolony. Our results show that the infiltration of non-cooperating cell types can substantially weaken the biofilm making it vulnerable to antibacterials and delay their pathogenesis. Cellulose, a component of EPS, was shown to play a pivotal role of acting as the main public good, and to edge-out the non-producers away from the cooperating microcolony.

  7. Inactivation of Salmonella enterica serovar Typhimurium on fresh produce by cold atmospheric gas plasma technology.

    Science.gov (United States)

    Fernández, A; Noriega, E; Thompson, A

    2013-02-01

    Cold atmospheric gas plasma treatment (CAP) is an alternative approach for the decontamination of fresh and minimally processed food. In this study, the effects of growth phase, growth temperature and chemical treatment regime on the inactivation of Salmonella enterica serovar Typhimurium (S. Typhimurium) by Nitrogen CAP were examined. Furthermore, the efficacy of CAP treatment for decontaminating lettuce and strawberry surfaces and potato tissue inoculated with S. Typhimurium was evaluated. It was found that the rate of inactivation of S. Typhimurium was independent of the growth phase, growth temperature and chemical treatment regime. Under optimal conditions, a 2 min treatment resulted in a 2.71 log-reduction of S. Typhimurium viability on membrane filters whereas a 15 min treatment was necessary to achieve 2.72, 1.76 and 0.94 log-reductions of viability on lettuce, strawberry and potato, respectively. We suggest that the differing efficiency of CAP treatment on the inactivation of S. Typhimurium on these different types of fresh foods is a consequence of their surface features. Scanning electron microscopy of the surface structures of contaminated samples of lettuce, strawberry and potato revealed topographical features whereby S. Typhimurium cells could be protected from the active species generated by plasma. Copyright © 2012 Elsevier Ltd. All rights reserved.

  8. Essential oils reduce Escherichia coli O157:H7 and Salmonella on spinach leaves without affecting produce quality

    Science.gov (United States)

    The efficacy of cinnamaldehyde and Sporan alone, or in combination with acetic acid in reducing E. coli O157:H7 and Salmonella on spinach leaves was investigated. Spinach leaves were inoculated with a cocktail of five-strain Salmonella or E. coli O157:H7, air-dried for 30 min, and then immersed in a...

  9. Biosurfactant Produced by Salmonella Enteritidis SE86 Can Increase Adherence and Resistance to Sanitizers on Lettuce Leaves (Lactuca sativa L., cichoraceae)

    OpenAIRE

    Rossi, Eliandra M.; Beilke, Luniele; Kochhann, Mar?lia; Sarzi, Diana H.; Tondo, Eduardo C.

    2016-01-01

    Salmonella Enteritidis SE86 is an important foodborne pathogen in Southern Brazil and it is able to produce a biosurfactant. However, the importance of this compound for the microorganism is still unknown. This study aimed to investigate the influence of the biosurfactant produced by S. Enteritidis SE86 on adherence to slices of lettuce leaves and on resistance to sanitizers. First, lettuce leaves were inoculated with S. Enteritidis SE86 in order to determine the amount of biosurfactant produ...

  10. Comparison of Sample and Detection Quantification Methods for Salmonella Enterica from Produce

    Science.gov (United States)

    Hummerick, M. P.; Khodadad, C.; Richards, J. T.; Dixit, A.; Spencer, L. M.; Larson, B.; Parrish, C., II; Birmele, M.; Wheeler, Raymond

    2014-01-01

    The purpose of this study was to identify and optimize fast and reliable sampling and detection methods for the identification of pathogens that may be present on produce grown in small vegetable production units on the International Space Station (ISS), thus a field setting. Microbiological testing is necessary before astronauts are allowed to consume produce grown on ISS where currently there are two vegetable production units deployed, Lada and Veggie.

  11. Risk Factors for Salmonella, Shiga Toxin-Producing Escherichia coli and Campylobacter Occurrence in Primary Production of Leafy Greens and Strawberries

    Directory of Open Access Journals (Sweden)

    Siele Ceuppens

    2015-08-01

    Full Text Available The microbiological sanitary quality and safety of leafy greens and strawberries were assessed in the primary production in Belgium, Brazil, Egypt, Norway and Spain by enumeration of Escherichia coli and detection of Salmonella, Shiga toxin-producing E. coli (STEC and Campylobacter. Water samples were more prone to containing pathogens (54 positives out of 950 analyses than soil (16/1186 and produce on the field (18/977 for leafy greens and 5/402 for strawberries. The prevalence of pathogens also varied markedly according to the sampling region. Flooding of fields increased the risk considerably, with odds ratio (OR 10.9 for Salmonella and 7.0 for STEC. A significant association between elevated numbers of generic E. coli and detection of pathogens (OR of 2.3 for STEC and 2.7 for Salmonella was established. Generic E. coli was found to be a suitable index organism for Salmonella and STEC, but to a lesser extent for Campylobacter. Guidelines on frequency of sampling and threshold values for E. coli in irrigation water may differ from region to region.

  12. Risk Factors for Salmonella, Shiga Toxin-Producing Escherichia coli and Campylobacter Occurrence in Primary Production of Leafy Greens and Strawberries

    Science.gov (United States)

    Ceuppens, Siele; Johannessen, Gro S.; Allende, Ana; Tondo, Eduardo César; El-Tahan, Fouad; Sampers, Imca; Jacxsens, Liesbeth; Uyttendaele, Mieke

    2015-01-01

    The microbiological sanitary quality and safety of leafy greens and strawberries were assessed in the primary production in Belgium, Brazil, Egypt, Norway and Spain by enumeration of Escherichia coli and detection of Salmonella, Shiga toxin-producing E. coli (STEC) and Campylobacter. Water samples were more prone to containing pathogens (54 positives out of 950 analyses) than soil (16/1186) and produce on the field (18/977 for leafy greens and 5/402 for strawberries). The prevalence of pathogens also varied markedly according to the sampling region. Flooding of fields increased the risk considerably, with odds ratio (OR) 10.9 for Salmonella and 7.0 for STEC. A significant association between elevated numbers of generic E. coli and detection of pathogens (OR of 2.3 for STEC and 2.7 for Salmonella) was established. Generic E. coli was found to be a suitable index organism for Salmonella and STEC, but to a lesser extent for Campylobacter. Guidelines on frequency of sampling and threshold values for E. coli in irrigation water may differ from region to region. PMID:26295251

  13. A mixture containing galactooligosaccharide, produced by the enzymic activity of Bifidobacterium bifidum, reduces Salmonella enterica serovar Typhimurium infection in mice.

    Science.gov (United States)

    Searle, Laura E J; Best, Angus; Nunez, Alejandro; Salguero, Francisco J; Johnson, Linda; Weyer, Ute; Dugdale, Alexandra H; Cooley, William A; Carter, Ben; Jones, Gareth; Tzortzis, George; Woodward, Martin J; La Ragione, Roberto M

    2009-01-01

    The prebiotic Bimuno is a mixture containing galactooligosaccharide, produced by the galactosyltransferase activity of Bifidobacterium bifidum NCIMB 41171 in the presence of lactose. Previous studies have implicated prebiotics in reducing infections by enteric pathogens, thus it was hypothesized that Bimuno may confer some protection in the murine host from Salmonella enterica serovar Typhimurium (S. Typhimurium) infection. In this study, infection caused by S. Typhimurium SL1344nal(r) in the presence or absence of Bimuno was assessed using tissue culture assays, a murine ligated ileal gut loop model and a murine oral challenge model. In tissue culture adherence and invasion assays with HT-29-16E cells, the presence of approximately 2 mM Bimuno significantly reduced the invasion of S. Typhimurium SL1344nal(r) (PBimuno prevented colonization and the associated pathology of S. Typhimurium. In the BALB/c mouse model, the oral delivery of Bimuno prior to challenge with S. Typhimurium resulted in significant reductions in colonization in the five organs sampled, with highly significant reductions being observed in the spleen at 72 and 96 h post-challenge (P=0.0002, Bimuno significantly reduced the colonization and pathology associated with S. Typhimurium infection in a murine model system, possibly by reducing the invasion of the pathogen into host cells.

  14. Resistance of Strains Producing Extended-Spectrum β-Lactamases Among Salmonella from Duck Carcasses at Slaughterhouses in Three Major Provinces of South Korea.

    Science.gov (United States)

    Lee, Soo Kyoung; Choi, Dasom; Chon, Jung Whan; Seo, Kun Ho

    2016-03-01

    The current study was carried out to estimate Salmonella spp. contamination of duck carcasses and to determine the antibiotic susceptibility profiles and serotype distribution of the isolates. Salmonella spp. was detected in 21.7% (26/120) of fresh raw duck carcasses sampled at different slaughterhouses in South Korea. Eight Salmonella serovars were identified; the most prevalent serovar was S. Typhimurium (34.6%), followed by S. Virchow (30.8%). All isolates were resistant to at least one antibiotic, and five remarkable isolates were resistant to more than 10 antibiotics, including third- and fourth-generation cephalosporins. Additional phenotypic and genetic characterization demonstrated that these isolates harbored resistance genes to broad-spectrum β-lactams, blaCTX-M-15 and blaCMY-2 genes, among the most prevalent β-lactamase enzymes worldwide. Based on molecular subtyping performed using the DiversiLab™ automated repetitive-sequence-based PCR system, isolates were classified into cluster A and cluster B. Among β-lactamase-producing Salmonellas, the isolate showing >98% similarity in their repetitive-sequence-based PCR banding pattern seemed to have acquired the resistance gene (blaCMY-2) and thus a distinct multiresistance profile. Given that antibiotic-resistant genes might be transferred by plasmid-mediated conjugation, periodic microbiological assessment within slaughterhouses is recommended for pathogens not to be transmitted through cross-contamination during slaughtering and dressing.

  15. Prevalence and characterization of Listeria monocytogenes, Salmonella and Shiga toxin-producing Escherichia coli isolated from small Mexican retail markets of queso fresco.

    Science.gov (United States)

    Soto Beltran, Marcela; Gerba, Charles P; Porto Fett, Anna; Luchansky, John B; Chaidez, Cristobal

    2015-01-01

    Queso fresco (QF) is a handmade cheese consumed and produced in Latin America. In Mexico, QF production is associated with a microbiological risk. The aim of the study was to determine the incidence and characterization of Listeria monocytogenes, Salmonella spp., and Shiga toxin-producing Escherichia coli (STEC) in QF from retail markets of the north-western State of Sinaloa, Mexico, and to assess the effect of physicochemical parameters on Listeria presence. A total of 75 QF samples were obtained. L. monocytogenes, E. coli, and coliforms were detected in 9.3, 94, and 100%, respectively. Salmonella was not detected. STEC isolates showed virulence genes. Microbial loads were above the maximum values recommended by the Official Mexican Standards. Physicochemical parameters such as water activity (aw), moisture content, pH, and salinity played a role in Listeria prevalence in QF. Rigorous control in QF made in Culiacan, Mexico is needed to reduce the risk of foodborne pathogens.

  16. OXA-48 carbapenemase-producing Salmonella enterica serovar Kentucky isolate of sequence type 198 in a patient transferred from Libya to Switzerland.

    Science.gov (United States)

    Seiffert, Salome N; Perreten, Vincent; Johannes, Sönke; Droz, Sara; Bodmer, Thomas; Endimiani, Andrea

    2014-01-01

    Here, we report a case of OXA-48-producing Salmonella enterica serovar Kentucky of sequence type 198 (ST198) from perianal screening cultures of a patient transferred from Libya to Switzerland. The blaOXA-48 gene was carried by Tn1999.2 and located on an ∼60-kb IncL/M plasmid. This Salmonella strain also possessed the blaVEB-8, aac(6)-Ib, tet(A), sul1, and mphA resistance genes and substitutions in GyrA (Ser83Phe and Asp87Asn) and ParC (Ser80Ile). This finding emphasizes that prompt screening strategies are essential to prevent the dissemination of carbapenemase producers imported from countries where they are endemic.

  17. Microbiological analysis of pre-packed sweet basil (Ocimum basilicum) and coriander (Coriandrum sativum) leaves for the presence of Salmonella spp. and Shiga toxin-producing E. coli.

    Science.gov (United States)

    Delbeke, Stefanie; Ceuppens, Siele; Jacxsens, Liesbeth; Uyttendaele, Mieke

    2015-09-02

    Enteric pathogens, such as Salmonella spp. and pathogenic Escherichia coli, have been detected and associated with food borne outbreaks from (imported) fresh leafy herbs. Screening on imported herbs from South East Asian countries has been described. However, limited information on prevalence of these pathogens is available from other sourcing regions. Therefore, fresh pre-packed basil and coriander leaves from a Belgian trading company were investigated for the presence of Salmonella spp., Shiga toxin-producing E. coli (STEC), generic E. coli and coliforms. In total 592 samples were collected originating from Belgium, Israel and Cyprus during 2013-2014. Multiplex PCR followed by further culture confirmation was used for the detection of Salmonella spp. and STEC, whereas the Petrifilm Select E. coli and VRBL-agar were used, respectively, for the enumeration of E. coli and coliforms. Salmonella was detected in 10 out of 592 samples (25g) (1.7%; 5 from basil and 5 from coriander), of which two samples were sourced from Israel and eight from Cyprus. The presence of STEC was suspected in 11 out of 592 samples (25g) (1.9%; 3 basil and 8 coriander), due to the detection of stx and eae genes, of which one sample originated from Belgium, four from Israel and six from Cyprus. No STEC was isolated by culture techniques, but in three samples a serotype (O26, O103 or O111) with its most likely associated eae-variant (β or θ) was detected by PCR. Generic E. coli was enumerated in 108 out of 592 samples, whereby 55, 32 and 13 samples respectively between 10-100, 100-1000 and 1000-10,000cfu/g and 8 samples exceeding 10,000cfu/g. Coliforms were enumerated in all herb samples at variable levels ranging from 1.6 to 7.5logcfu/g. Further statistics indicate that the E. coli class (categorized by level) was significantly correlated with the presence of Salmonella (p<0.001) or STEC (p=0.019), while coliform counts were significant correlated with Salmonella (p<0.001), but not with

  18. Combats escènics

    Directory of Open Access Journals (Sweden)

    Pawel Rouba Billowicz

    2016-04-01

    Full Text Available “Combats Escènics” és un treball que tracta sobre la interpretació artística de la violència d’artistes de l’espectacle per tal de divertir el públic i emetre un missatge humanista mitjançant una coreografia ritual. En aquest estudi es presenta una classificació del combat escènic des del doble vessant agonista/antagonista, es realitza un passeig històric de la representació artística del combat a través de les diferents etapes i de les diverses cultures, s’aborda la preparació escènica de l’actor i del coreògraf, i s’entreveuen les perspectives de futur d’aquesta modalitat artística. Estudi realitzat per Pawel Rouba Billewicz (Inowroclaw, Polònia, 1939 - Barcelona, 2007, director, coreògraf, actor, mestre d’armes, mestre del gest i de la pantomima i professor de l’INEF de Catalunya. Aquest article, editorialment inèdit, es publica postmortem per Apunts. Educació Física i Esports com a homenatge i reconeixement de l’autor per la seva extraordinària i polivalent aportació al camp de l’art i l’Activitat Física i l’Esport.

  19. CTX-M extended-spectrum β-lactamase-producing Klebsiella spp, Salmonella spp, Shigella spp and Escherichia coli isolates in Iranian hospitals.

    Science.gov (United States)

    Bialvaei, Abed Zahedi; Kafil, Hossein Samadi; Asgharzadeh, Mohammad; Aghazadeh, Mohammad; Yousefi, Mehdi

    2016-01-01

    This study was conducted in Iran in order to assess the distribution of CTX-M type ESBLs producing Enterobacteriaceae. From January 2012 to December 2013, totally 198 E. coli, 139 Klebsiella spp, 54 Salmonella spp and 52 Shigella spp from seven hospitals of six provinces in Iran were screened for resistance to extended-spectrum cephalosporins. After identification and susceptibility testing, isolates presenting multiple-drug resistance (MDR) were evaluated for ESBL production by the disk combination method and by Etest using (cefotaxime and cefotaxime plus clavulanic acid). All isolates were also screened for blaCTX-M using conventional PCR. A total of 42.92%, 33.81%, 14.81% and 7.69% of the E. coli, Klebsiella spp, Salmonella spp and Shigella spp isolates were MDR, respectively. The presence of CTX-M enzyme among ESBL-producing isolates was 85.18%, 77.7%, 50%, and 66.7%, in E. coli, Klebsiella spp, Salmonella spp and Shigella spp respectively. The overall presence of CTX-M genes in Enterobacteriaceae was 15.4% and among the resistant isolates was 47.6%. This study indicated that resistance to β-lactams mediated by CTX-M enzymes in Iran had similar pattern as in other parts of the world. In order to control the spread of resistance, comprehensive studies and programs are needed. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  20. Eradication of Salmonella and Arizona species from turtle hatchlings produced from eggs treated on commercial turtle farms.

    OpenAIRE

    Siebeling, R J; Caruso, D.; Neuman, S

    1984-01-01

    On commercial turtle farms more than 40% of the hatchlings excrete detectable levels of Salmonella and Arizona spp. when hatched from nonsanitized eggs incubated in sawdust or dirt-filled chambers. Over a 3-year period on 10 farms, more than 10(6) turtle eggs were treated in an attempt to hatch Salmonella-free turtles. Eggs were sanitized in disinfectant, treated by temperature- or pressure-differential dip methods in solutions containing 500 micrograms or more of gentamicin sulfate per ml, a...

  1. Increased detection of extended spectrum beta-lactamase producing Salmonella enterica and Escherichia coli isolates from poultry

    NARCIS (Netherlands)

    Dierikx, C.M.; Essen-Zandbergen, van A.; Veldman, K.T.; Smith, H.E.; Mevius, D.J.

    2010-01-01

    To gain more information on the genetic basis of the rapid increase in the number of isolates exhibiting non-wild type Minimum Inhibitory Concentrations (MICs) for cefotaxime observed since 2003, beta-lactamase genes of 22 Salmonella enterica and 22 Escherichia coli isolates from broilers in 2006

  2. Biosurfactant Produced by Salmonella Enteritidis SE86 Can Increase Adherence and Resistance to Sanitizers on Lettuce Leaves (Lactuca sativa L., cichoraceae).

    Science.gov (United States)

    Rossi, Eliandra M; Beilke, Luniele; Kochhann, Marília; Sarzi, Diana H; Tondo, Eduardo C

    2016-01-01

    Salmonella Enteritidis SE86 is an important foodborne pathogen in Southern Brazil and it is able to produce a biosurfactant. However, the importance of this compound for the microorganism is still unknown. This study aimed to investigate the influence of the biosurfactant produced by S. Enteritidis SE86 on adherence to slices of lettuce leaves and on resistance to sanitizers. First, lettuce leaves were inoculated with S. Enteritidis SE86 in order to determine the amount of biosurfactant produced. Subsequently, lettuce leaves were inoculated with S. Enteritidis SE86 with and without the biosurfactant, and the adherence and bacterial resistance to different sanitization methods were evaluated. S. Enteritidis SE86 produced biosurfactant after 16 h (emulsification index of 11 to 52.15 percent, P lettuce leaves' stomata in the presence of the biosurfactant. Results indicated that the biosurfactant produced by S. Enteritidis SE86 contributed to adherence and increased resistance to sanitizers when the microorganism was present on lettuce leaves.

  3. Impact of antibiotics on the intestinal microbiota and on the treatment of Shiga-toxin-producing Escherichia coli and Salmonella infections.

    Science.gov (United States)

    Szych, Jolanta; Wołkowicz, Tomasz; La Ragione, Roberto; Madajczak, Grzegorz

    2014-01-01

    This review evaluates the current literature based on the impact of antibiotics on the intestinal microbiota and the critical role of intestinal bacteria in controlling infection and subsequent clinical disease caused by STEC and Salmonella, and the transmissibility of these important pathogens.A number of studies have indicated that antibiotic therapy could result in unexpected changes in the clinical picture of disease. This is observed, for example, in the case of infections associated with Shiga-toxin-producing Escherichia coli (STEC), when antibiotics used in treatment of the disease may increase the risk of hemolytic uremic syndrome (HUS) and thus fatal outcomes. In the case of such infections, treatment with antibiotics is usually discouraged. The use of antibiotics could cause also undesirable changes in the intestinal microbial flora and prolonged pathogen shedding, which is observed in the case of Salmonella infections. Inappropriate antibiotic therapy can result in Salmonella remaining in the host's cells (intracellular) and thus resulting in further asymptomatic carriage and a further complication is the development of resistance.

  4. The antibiotic resistance characteristics of non-typhoidal Salmonella enterica isolated from food-producing animals, retail meat and humans in South East Asia.

    Science.gov (United States)

    Van, Thi Thu Hao; Nguyen, Hoang Nam Kha; Smooker, Peter M; Coloe, Peter J

    2012-03-15

    Antimicrobial resistance is a global problem. It is most prevalent in developing countries where infectious diseases remain common, the use of antibiotics in humans and animals is widespread, and the replacement of older antibiotics with new generation antibiotics is not easy due to the high cost. Information on antibiotic resistance phenotypes and genotypes of Salmonella spp. in food animals and humans in different countries and geographic regions is necessary to combat the spread of resistance. This will improve the understanding of antibiotic resistance epidemiology, tracing of new emerging pathogens, assisting in disease treatment, and enhancing prudent use of antibiotics. However, the extent of antibiotic resistance in food-borne pathogens and humans in many developing countries remains unknown. The goal of this review is to discuss the current state of antibiotic resistance of non-typhoid Salmonella spp. in food-producing animals, retail meat and humans from South East Asia. It is focused on resistance characteristics of traditional and "critically important" antibiotics in this region, and the emergence of multidrug resistant strains and genetic elements that contribute to the development of multidrug resistance, including integrons and the Salmonella Genomic Island (SGI). Copyright © 2011. Published by Elsevier B.V.

  5. Prevalence of shiga toxin producing Escherichia coli, Salmonella enterica, and Listeria monocytogenes at public access watershed sites in a California Central Coast agricultural region

    Science.gov (United States)

    Cooley, Michael B.; Quiñones, Beatriz; Oryang, David; Mandrell, Robert E.; Gorski, Lisa

    2014-01-01

    Produce contaminated with enteric pathogens is a major source of foodborne illness in the United States. Lakes, streams, rivers, and ponds were sampled with Moore swabs bi-monthly for over 2 years at 30 locations in the vicinity of a leafy green growing region on the Central California Coast and screened for Shiga toxin producing Escherichia coli (STEC), Salmonella enterica, and Listeria monocytogenes to evaluate the prevalence and persistence of pathogen subtypes. The prevalence of STEC from 1386 samples was 11%; 110 samples (8%) contained E. coli O157:H7 with the highest prevalence occurring close to cattle operations. Non-O157 STEC isolates represented major clinical O-types and 57% contained both shiga toxin types 1 and 2 and intimin. Multiple Locus Variable Number Tandem Repeat Analysis of STEC isolates indicated prevalent strains during the period of study. Notably, Salmonella was present at high levels throughout the sampling region with 65% prevalence in 1405 samples resulting in 996 isolates with slightly lower prevalence in late autumn. There were 2, 8, and 14 sites that were Salmonella-positive over 90, 80, and 70% of the time, respectively. The serotypes identified most often were 6,8:d:-, Typhimurium, and Give. Interestingly, analysis by Pulsed Field Gel Electrophoresis indicated persistence and transport of pulsotypes in the region over several years. In this original study of L. monocytogenes in the region prevalence was 43% of 1405 samples resulting in 635 individual isolates. Over 85% of the isolates belonged to serotype 4b with serotypes 1/2a, 1/2b, 3a, 4d with 4e representing the rest, and there were 12 and 2 sites that were positive over 50 and 80% of the time, respectively. Although surface water is not directly used for irrigation in this region, transport to the produce can occur by other means. This environmental survey assesses initial contamination levels toward an understanding of transport leading to produce recalls or outbreaks. PMID

  6. Ability of Shiga toxin-producing Escherichia coli and Salmonella spp. to survive in a desiccation model system and in dry foods.

    Science.gov (United States)

    Hiramatsu, Reiji; Matsumoto, Masakado; Sakae, Kenji; Miyazaki, Yutaka

    2005-11-01

    In order to determine desiccation tolerances of bacterial strains, the survival of 58 diarrheagenic strains (18 salmonellae, 35 Shiga toxin-producing Escherichia coli [STEC], and 5 shigellae) and of 15 nonpathogenic E. coli strains was determined after drying at 35 degrees C for 24 h in paper disks. At an inoculum level of 10(7) CFU/disk, most of the salmonellae (14/18) and the STEC strains (31/35) survived with a population of 10(3) to 10(4) CFU/disk, whereas all of the shigellae (5/5) and the majority of the nonpathogenic E. coli strains (9/15) did not survive (the population was decreased to less than the detection limit of 10(2) CFU/disk). After 22 to 24 months of subsequent storage at 4 degrees C, all of the selected salmonellae (4/4) and most of the selected STEC strains (12/15) survived, keeping the original populations (10(3) to 10(4) CFU/disk). In contrast to the case for storage at 4 degrees C, all of 15 selected strains (5 strains each of Salmonella spp., STEC O157, and STEC O26) died after 35 to 70 days of storage at 25 degrees C and 35 degrees C. The survival rates of all of these 15 strains in paper disks after the 24 h of drying were substantially increased (10 to 79 times) by the presence of sucrose (12% to 36%). All of these 15 desiccated strains in paper disks survived after exposure to 70 degrees C for 5 h. The populations of these 15 strains inoculated in dried foods containing sucrose and/or fat (e.g., chocolate) were 100 times higher than those in the dried paper disks after drying for 24 h at 25 degrees C.

  7. Multiplex real-time PCR and culture methods for detection of Shiga toxin-producing Escherichia coli and Salmonella Thompson in strawberries, a lettuce mix and basil.

    Science.gov (United States)

    Delbeke, S; Ceuppens, S; Holvoet, K; Samuels, E; Sampers, I; Uyttendaele, M

    2015-01-16

    An appropriate approach of high throughput multi-screening was verified for Shiga toxin-producing Escherichia coli (STEC) and Salmonella spp. in strawberries, lettuce and basil. Sample replicates were inoculated with STEC O157 or O26 and Salmonella Thompson (ca. 10-70, 100-700 and 1000-7000 cfu/25 g) and analysed after 1 and 5 days of storage (strawberries and lettuce at 7 °C and basil at 10 °C). After 18-24 h of enrichment at 37 °C in buffered peptone water, detection was performed using the GeneDisc multiplex PCR (stx1, stx2, eae and iroB genes) and selective culture media for isolation of STEC (with immunomagnetic separation (IMS)) and Salmonella spp. in parallel. After 1 day, the pathogenic strains were recovered from all samples for all inoculum levels, whereas reduced detection rates of STEC O157 and S. Thompson were observed after 5 days of storage in case of strawberries, in particular for the lowest inoculums level, suggesting superior survival potential for STEC O26. Overall, this study indicates the ability of PCR based screening methods for reproducible multi-detection of low numbers (10-70 cfu/25 g) of STEC and Salmonella in this type of foods. However, for the basil samples, PCR needed twofold dilution of the DNA extract to overcome inhibition. It was noted that on several occasions growth of competitive microbiota obstructed finding presumptive colonies on the selective agar media, whereas the use of an additional agar medium such as CHROMagar STEC (without IMS) improved recovery rate of STEC. Copyright © 2014 Elsevier B.V. All rights reserved.

  8. Enhanced inactivation of Salmonella and Pseudomonas biofilms on stainless steel by use of T-128, a fresh-produce washing aid, in chlorinated wash solutions.

    Science.gov (United States)

    Shen, Cangliang; Luo, Yaguang; Nou, Xiangwu; Bauchan, Gary; Zhou, Bin; Wang, Qin; Millner, Patricia

    2012-10-01

    The effect of the washing aid T-128 (generally recognized as safe [GRAS] formulation, composed mainly of phosphoric acid and propylene glycol) on inactivation of Salmonella and Pseudomonas populations in biofilms on stainless steel was evaluated under conditions of increasing organic matter loads in chlorinated wash solutions dominated by hypochlorous acid. Biofilms were formed statically on stainless steel coupons suspended in 2% lettuce extract after inoculation with Salmonella enterica serovar Thompson or Newport or with Pseudomonas fluorescens. Coupons with biofilms were washed in chlorine solutions (0, 0.5, 1, 2, 5, 10, or 20 mg/liter at pH 6.5, 5.0 and 2.9), with or without T-128, and with increasing loads of organic matter (0, 0.25, 0.5, 0.75, or 1.0% lettuce extract). Cell populations on coupons were dispersed using intermittent, pulsed ultrasonication and vortexing and enumerated by colony counts on XLT-4 or Pseudomonas agars. Cell responses to fluorescent viability staining of biofilm treatment washing solutions were examined using confocal laser scanning microscopy. Results showed that 0.1% T-128 (without chlorine) reduced P. fluorescens biofilm populations by 2.5 log(10) units but did not reduce Salmonella populations. For both Salmonella and Pseudomonas, the sanitizing effect of free chlorine (1.0 to 5.0 mg/liter) was enhanced (P biofilms compared to treatments without T-128. Image analysis of surfaces stained with SYTO and propidium iodide corroborate the cultural assay results showing that T-128 can aid in reducing pathogen viability in biofilms and thus can aid in sanitizing stainless steel contact surfaces during processing of fresh-cut produce.

  9. Occurrence, characterization, and potential predictors of verotoxigenic Escherichia coli, Listeria monocytogenes, and Salmonella in surface water used for produce irrigation in the Lower Mainland of British Columbia, Canada.

    Directory of Open Access Journals (Sweden)

    Justin Falardeau

    Full Text Available Produce has become a major source of foodborne illness, and may become contaminated through surface water irrigation. The objectives of this study were to (i determine the frequency of verotoxigenic E. coli (VTEC, Listeria monocytogenes, and Salmonella in surface waters used for irrigation in the Lower Mainland of British Columbia, (ii assess the suitability of fecal coliforms and generic E. coli as hygiene indicators, and (iii investigate the correlations of environmental factors with pathogen occurrence. Water samples were collected semi-monthly for 18 months from seven irrigation ditches across the Serpentine and Sumas watersheds. VTEC colonies on water filters were detected using a verotoxin colony immunoblot, and the presence of virulence genes vt1 and vt2 was ascertained via multiplex PCR. Detection of L. monocytogenes and Salmonella was completed using standard, Health Canada Compendium of Analytical Methods. Fecal coliforms and generic E. coli were enumerated by 3M™ Petrifilm™ and filtration methods, and meteorological and geographic data were collected from government records. VTEC, L. monocytogenes, and Salmonella were detected in 4.93%, 10.3%, and 2.69% of 223 samples, respectively. L. monocytogenes occurrence was greatest in the Serpentine watershed (χ2; p < 0.05, and was most common during the winter and fall (Fisher exact test; p < 0.05. Site dependence of VTEC and Salmonella occurrence was observed within watersheds (Fisher's exact test; p < 0.10. Pathogen occurrence correlated with fecal coliform counts (r = 0.448, while VTEC occurrence also correlated with precipitation over the five days before sampling (r = 0.239. The density of upstream livestock correlated with VTEC (rs = 0.812, and L. monocytogenes (rs = 0.841 detection. These data show that foodborne pathogens are present in the waters used for irrigation in the Lower Mainland of British Columbia, but their frequency may depend on spatial and temporal factors.

  10. Multiple transmissible genes encoding fluoroquinolone and third-generation cephalosporin resistance co-located in non-typhoidal Salmonella isolated from food-producing animals in China.

    Science.gov (United States)

    Jiang, Hong-Xia; Song, Li; Liu, Ji; Zhang, Xiao-Hua; Ren, Yan-Na; Zhang, Wen-Hui; Zhang, Jing-Yuan; Liu, Ya-Hong; Webber, Mark A; Ogbolu, David O; Zeng, Zhen-Ling; Piddock, Laura J V

    2014-03-01

    The aim of this study was to identify genes conferring resistance to fluoroquinolones and extended-spectrum β-lactams in non-typhoidal Salmonella (NTS) from food-producing animals in China. In total, 31 non-duplicate NTS were obtained from food-producing animals that were sick. Isolates were identified and serotyped and the genetic relatedness of the isolates was determined by pulsed-field gel electrophoresis of XbaI-digested chromosomal DNA. Antimicrobial susceptibility was determined using Clinical and Laboratory Standards Institute methodology. The presence of extended-spectrum β-lactamase (ESBL) and fluoroquinolone resistance genes was established by PCR and sequencing. Genes encoded on transmissible elements were identified by conjugation and transformation. Plasmids were typed by PCR-based replicon typing. The occurrence and diversity of numerous different transmissible genes conferring fluoroquinolone resistance [qnrA, qnrD, oqxA and aac(6')-Ib-cr] and ESBLs (CTX-M-27 and CTX-M-14), and which co-resided in different isolates and serovars of Salmonella, were much higher than in European countries. Furthermore, different plasmids encoded fluoroquinolone resistance (ca. 6 kb) and β-lactam resistance (ca. 63 kb) and these co-resided in isolates with mutations in topoisomerase genes (gyrA and parC) giving very resistant Salmonella. The presence of multidrug-resistant bacteria in food-producing animals in countries that export foodstuffs suggests that global transfer of antibiotic resistances from country to country on food is possible. Copyright © 2013 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

  11. Inhibition of Escherichia coli O157:H7 and Salmonella enterica on spinach and identification of antimicrobial substances produced by a commercial Lactic Acid Bacteria food safety intervention.

    Science.gov (United States)

    Cálix-Lara, Thelma F; Rajendran, Mahitha; Talcott, Stephen T; Smith, Stephen B; Miller, Rhonda K; Castillo, Alejandro; Sturino, Joseph M; Taylor, T Matthew

    2014-04-01

    The microbiological safety of fresh produce is of concern for the U.S. food supply. Members of the Lactic Acid Bacteria (LAB) have been reported to antagonize pathogens by competing for nutrients and by secretion of substances with antimicrobial activity, including organic acids, peroxides, and antimicrobial polypeptides. The objectives of this research were to: (i) determine the capacity of a commercial LAB food antimicrobial to inhibit Escherichia coli O157:H7 and Salmonella enterica on spinach leaf surfaces, and (ii) identify antimicrobial substances produced in vitro by the LAB comprising the food antimicrobial. Pathogens were inoculated on freshly harvested spinach, followed by application of the LAB antimicrobial. Treated spinach was aerobically incubated up to 12 days at 7 °C and surviving pathogens enumerated via selective/differential plating. l-Lactic acid and a bacteriocin-like inhibitory substance (BLIS) were detected and quantified from cell-free fermentates obtained from LAB-inoculated liquid microbiological medium. Application of 8.0 log10 CFU/g LAB produced significant (p < 0.05) reductions in E. coli O157:H7 and Salmonella populations on spinach of 1.6 and 1.9 log10 CFU/g, respectively. It was concluded the LAB antimicrobial inhibited foodborne pathogens on spinach during refrigerated storage, likely the result of the production of metabolites with antimicrobial activity. Copyright © 2013 Elsevier Ltd. All rights reserved.

  12. Growth of Escherichia coli O157:H7, Non-O157 Shiga Toxin-Producing Escherichia coli , and Salmonella in Water and Hydroponic Fertilizer Solutions.

    Science.gov (United States)

    Shaw, Angela; Helterbran, Kara; Evans, Michael R; Currey, Christopher

    2016-12-01

    The desire for local, fresh produce year round is driving the growth of hydroponic growing systems in the United States. Many food crops, such as leafy greens and culinary herbs, grown within hydroponics systems have their root systems submerged in recirculating nutrient-dense fertilizer solutions from planting through harvest. If a foodborne pathogen were introduced into this water system, the risk of contamination to the entire crop would be high. Hence, this study was designed to determine whether Escherichia coli O157:H7, non-O157 Shiga toxin-producing E. coli , and Salmonella were able to survive and reproduce in two common hydroponic fertilizer solutions and in water or whether the bacteria would be killed or suppressed by the fertilizer solutions. All the pathogens grew by 1 to 6 log CFU/ml over a 24-h period, depending on the solution. E. coli O157:H7 reached higher levels in the fertilizer solution with plants (3.12 log CFU/ml), whereas non-O157 Shiga toxin-producing E. coli and Salmonella reached higher levels in the fertilizer solution without plants (1.36 to 3.77 log CFU/ml). The foodborne pathogens evaluated here survived for 24 h in the fertilizer solution, and populations grew more rapidly in these solutions than in plain water. Therefore, human pathogens entering the fertilizer solution tanks in hydroponic systems would be expected to rapidly propagate and spread throughout the system and potentially contaminate the entire crop.

  13. Meta-analysis of the Effects of Sanitizing Treatments on Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes Inactivation in Fresh Produce

    Science.gov (United States)

    Prado-Silva, Leonardo; Cadavez, Vasco; Gonzales-Barron, Ursula; Rezende, Ana Carolina B.

    2015-01-01

    The aim of this study was to perform a meta-analysis of the effects of sanitizing treatments of fresh produce on Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes. From 55 primary studies found to report on such effects, 40 were selected based on specific criteria, leading to more than 1,000 data on mean log reductions of these three bacterial pathogens impairing the safety of fresh produce. Data were partitioned to build three meta-analytical models that could allow the assessment of differences in mean log reductions among pathogens, fresh produce, and sanitizers. Moderating variables assessed in the meta-analytical models included type of fresh produce, type of sanitizer, concentration, and treatment time and temperature. Further, a proposal was done to classify the sanitizers according to bactericidal efficacy by means of a meta-analytical dendrogram. The results indicated that both time and temperature significantly affected the mean log reductions of the sanitizing treatment (P lettuce) compared to other, nonleafy vegetables (for example, 3.04 mean log reductions [2.32 to 3.76] obtained for carrots). Among the pathogens, E. coli O157:H7 was more resistant to ozone (1.6 mean log reductions), while L. monocytogenes and Salmonella presented high resistance to organic acids, such as citric acid, acetic acid, and lactic acid (∼3.0 mean log reductions). With regard to the sanitizers, it has been found that slightly acidic electrolyzed water, acidified sodium chlorite, and the gaseous chlorine dioxide clustered together, indicating that they possessed the strongest bactericidal effect. The results reported seem to be an important achievement for advancing the global understanding of the effectiveness of sanitizers for microbial safety of fresh produce. PMID:26362982

  14. Occurrence, characterization, and potential predictors of verotoxigenic Escherichia coli, Listeria monocytogenes, and Salmonella in surface water used for produce irrigation in the Lower Mainland of British Columbia, Canada

    Science.gov (United States)

    Falardeau, Justin; Johnson, Roger P.; Pagotto, Franco

    2017-01-01

    Produce has become a major source of foodborne illness, and may become contaminated through surface water irrigation. The objectives of this study were to (i) determine the frequency of verotoxigenic E. coli (VTEC), Listeria monocytogenes, and Salmonella in surface waters used for irrigation in the Lower Mainland of British Columbia, (ii) assess the suitability of fecal coliforms and generic E. coli as hygiene indicators, and (iii) investigate the correlations of environmental factors with pathogen occurrence. Water samples were collected semi-monthly for 18 months from seven irrigation ditches across the Serpentine and Sumas watersheds. VTEC colonies on water filters were detected using a verotoxin colony immunoblot, and the presence of virulence genes vt1 and vt2 was ascertained via multiplex PCR. Detection of L. monocytogenes and Salmonella was completed using standard, Health Canada Compendium of Analytical Methods. Fecal coliforms and generic E. coli were enumerated by 3M™ Petrifilm™ and filtration methods, and meteorological and geographic data were collected from government records. VTEC, L. monocytogenes, and Salmonella were detected in 4.93%, 10.3%, and 2.69% of 223 samples, respectively. L. monocytogenes occurrence was greatest in the Serpentine watershed (χ2; p coliform counts (r = 0.448), while VTEC occurrence also correlated with precipitation over the five days before sampling (r = 0.239). The density of upstream livestock correlated with VTEC (rs = 0.812), and L. monocytogenes (rs = 0.841) detection. These data show that foodborne pathogens are present in the waters used for irrigation in the Lower Mainland of British Columbia, but their frequency may depend on spatial and temporal factors. PMID:28953937

  15. Prevalence of Shiga toxin-producing Escherichia coli, Salmonella spp. and Campylobacter spp. in large game animals intended for consumption: relationship with management practices and livestock influence.

    Science.gov (United States)

    Díaz-Sánchez, S; Sánchez, S; Herrera-León, S; Porrero, C; Blanco, J; Dahbi, G; Blanco, J E; Mora, A; Mateo, R; Hanning, I; Vidal, D

    2013-05-03

    Although wild ruminants have been identified as reservoirs of Shiga-toxin producing Escherichia coli (STEC), little information is available concerning the role of Salmonella spp. and Campylobacter spp. in large game species. We evaluated the presence of these pathogens in faeces (N=574) and carcasses (N=585) sampled from red deer (N=295), wild boar (N=333) and other ungulates (fallow deer, mouflon) (N=9). Animal sampling was done in situ from 33 hunting estates during two hunting seasons. Salmonella spp. and Campylobacter spp. strains associated with human campylobacteriosis were infrequently detected indicating that both pathogens had a limited zoonotic risk in our study area. The overall STEC prevalence in animals was 21% (134/637), being significantly higher in faeces from red deer (90 out of 264). A total of 58 isolates were serotyped. Serotypes O146:H- and O27:H30 were the most frequent in red deer and the majority of isolates from red deer and wild boar were from serotypes previously found in STEC strains associated with human infection, including the serotype O157:H7. The STEC prevalence in red deer faeces was significantly higher with the presence of livestock (p<0, 01) where high densities of red deer (p<0.001) were present. To the best of our knowledge, this is the first study reporting the occurrence of Salmonella spp. and STEC in carcasses of large game animals. Furthermore, this study confirmed by pulsed-field gel electrophoresis (PFGE) that cross contamination of STEC during carcass dressing occurred, implying the likelihood of these pathogens entering into the food chain. Copyright © 2013 Elsevier B.V. All rights reserved.

  16. Quantifying transfer rates of Salmonella and Escherichia coli O157:H7 between fresh-cut produce and common kitchen surfaces.

    Science.gov (United States)

    Jensen, Dane A; Friedrich, Loretta M; Harris, Linda J; Danyluk, Michelle D; Schaffner, Donald W

    2013-09-01

    Cross-contamination between foods and surfaces in food processing environments and home kitchens may play a significant role in foodborne disease transmission. This study quantifies the cross-contamination rates between a variety of fresh-cut produce and common kitchen surfaces (ceramic, stainless steel, glass, and plastic) using scenarios that differ by cross-contamination direction, surface type, produce type, and drying time/moisture level. A five-strain cocktail of rifampin-resistant Salmonella was used in transfer scenarios involving celery, carrot, and watermelon, and a five-strain cocktail of rifampin-resistant Escherichia coli O157:H7 was used in transfer scenarios involving lettuce. Produce or surface coupons were placed in buffer-filled filter bags and homogenized or massaged, respectively, to recover cells. The resulting solutions were serially diluted in 0.1% peptone and surface plated onto tryptic soy agar with 80 μg/ml rifampin and bismuth sulfite agar with 80 μg/ml rifampin for Salmonella or sorbitol MacConkey agar with 80 μg/ml rifampin for E. coli O157:H7. When the food contact surface was freshly inoculated, a high amount (>90%) of the inoculum was almost always transferred to the cut produce item. If the inoculated food contact surfaces were allowed to dry for 1 h, median transfer was generally >90% for carrots and watermelon but ranged from lettuce. Freshly inoculated celery or lettuce transferred more bacteria (lettuce was <0.01 to ∼5% and <1 to ∼5% for watermelon. Surface moisture and direction of transfer have the greatest influence on microbial transfer rates.

  17. Biosurfactant produced by Salmonella Enteritidis SE86 can increase the adherence and resistance to sanitizers on lettuce leaves (Lactuca sativa L., cichoraceae

    Directory of Open Access Journals (Sweden)

    Eliandra Mirlei Rossi

    2016-01-01

    Full Text Available Salmonella Enteritidis SE86 is an important foodborne pathogen in Southern Brazil and it is able to produce a biosurfactant. However, the importance of this compound for the microorganism is still unknown. This study aimed to investigate the influence of biosurfactant produced by S. Enteritidis SE86 on the adherence to slices of lettuce leaves and on the resistance to sanitizers. First, S. Enteritidis SE86 was inoculated on lettuce leaves in order to determine the amount of biosurfactant produced. Subsequently, S. Enteritidis SE86 was inoculated on lettuce leaves, with and without the biosurfactant, and the adherence and bacterial resistance to different sanitization methods were evaluated. S. Enteritidis SE86 produced biosurfactant after 16 hours (emulsification index of 11 to 52.15% and showed greater adherence capability and resistance to sanitization methods when the compound was present. The scanning electron microscopy demonstrated that S. Enteritidis was able to adhere, form lumps, and invade the lettuce leaves stomata in the presence of biosurfactant. Results indicated that the biosurfactant produced by S. Enteritidis SE86 contributed to the adherence and increased the resistance to sanitizers when the microorganism was present on lettuce leaves.

  18. Biosurfactant Produced by Salmonella Enteritidis SE86 Can Increase Adherence and Resistance to Sanitizers on Lettuce Leaves (Lactuca sativa L., cichoraceae)

    Science.gov (United States)

    Rossi, Eliandra M.; Beilke, Luniele; Kochhann, Marília; Sarzi, Diana H.; Tondo, Eduardo C.

    2016-01-01

    Salmonella Enteritidis SE86 is an important foodborne pathogen in Southern Brazil and it is able to produce a biosurfactant. However, the importance of this compound for the microorganism is still unknown. This study aimed to investigate the influence of the biosurfactant produced by S. Enteritidis SE86 on adherence to slices of lettuce leaves and on resistance to sanitizers. First, lettuce leaves were inoculated with S. Enteritidis SE86 in order to determine the amount of biosurfactant produced. Subsequently, lettuce leaves were inoculated with S. Enteritidis SE86 with and without the biosurfactant, and the adherence and bacterial resistance to different sanitization methods were evaluated. S. Enteritidis SE86 produced biosurfactant after 16 h (emulsification index of 11 to 52.15 percent, P < 0.05) and showed greater adherence capability and resistance to sanitization methods when the compound was present. The scanning electron microscopy demonstrated that S. Enteritidis was able to adhere, form lumps, and invade the lettuce leaves’ stomata in the presence of the biosurfactant. Results indicated that the biosurfactant produced by S. Enteritidis SE86 contributed to adherence and increased resistance to sanitizers when the microorganism was present on lettuce leaves. PMID:26834727

  19. Salmonella Osteomyelitis

    National Research Council Canada - National Science Library

    McAnearney, S; McCall, D

    2015-01-01

    .... Salmonella as an aetiological agent in osteomyelitis is essentially rare and salmonella osteomyelitis in itself is predominantly seen in patients with haemoglobinopathies such as sickle cell disease or thalassemia...

  20. Outbreak of Salmonella serovar Stanley infections in Switzerland linked to locally produced soft cheese, September 2006 - February 2007.

    Science.gov (United States)

    Pastore, R; Schmid, H; Altpeter, E; Baumgartner, A; Hächler, H; Imhof, R; Sudre, P; Boubaker, K

    2008-09-11

    Salmonella serovar Stanley is rare in Europe. In Switzerland, the number of reported isolates has increased from 2 in 2000 to 25 in 2005. A nationwide outbreak of gastrointestinal illness due to S. Stanley occurred from September 2006 through February 2007. Eighty-two cases were documented. Males were 56%; mean age of the cases was 45.7 years (range 0-92). Forty-seven cases (57%) occurred in three western cantons: Vaud, Bern, and Geneva. Twenty-three cases (28%) were hospitalised. In the case-control study conducted to find the source of the outbreak, cases were more likely than controls to have eaten local soft cheese (OR 11.4, p=0.008). One clone of S. Stanley strain was isolated from soft cheese and from 77 cases (94%) who reported no history of having travelled abroad. The outbreak ended after the withdrawal of the cheese from the market. This is the first S. Stanley outbreak in Switzerland and the first in Europe unrelated to imported products, suggesting an increased local circulation of this previously rare serotype.

  1. In Silico Docking of Small-Molecule Inhibitors to the Escherichia coli Type III Secretion System EscN ATPase

    Science.gov (United States)

    2014-07-01

    proteins directly into host cells, which permits pathogen survival and replication and evades the host immune response at the same time. These pathogens...ATPases that were tested include EscN (enteropathogenic Escherichia coli), InvC ( Salmonella ), Spa47 (Shigella flexneri), BsaS and SpaL (Burkholderia...2 panel for broad-spectrum activity by measuring their ability to confer macrophage survivability against infection by human pathogens. We

  2. Evaluation of the performance of the IQ-check kits and the USDA microbiology laboratory guidebook methods for detection of Shiga Toxin-Producing E. coli (STEC) and STEC and Salmonella simultaneously in ground beef

    Science.gov (United States)

    Aims: To evaluate the performance of the IQ-Check kits and the USDA Microbiology Laboratory Guidebook (MLG) methods for detection of the top 7 Shiga toxin-producing E. coli (STEC) (O157:H7, O26, O45, O103, O111, O121, and O145) in ground beef and both STEC and Salmonella in co-inoculated samples. M...

  3. Genomic Dissection of Travel-Associated Extended-Spectrum-Beta-Lactamase-Producing Salmonella enterica Serovar Typhi Isolates Originating from the Philippines: a One-Off Occurrence or a Threat to Effective Treatment of Typhoid Fever?

    DEFF Research Database (Denmark)

    Hendriksen, Rene S.; Leekitcharoenphon, Pimlapas; Mikoleit, Matthew

    2015-01-01

    One unreported case of extended-spectrum-beta-lactamase (ESBL)-producing Salmonella enterica serovar Typhi was identified, whole-genome sequence typed, among other analyses, and compared to other available genomes of S. Typhi. The reported strain was similar to a previously published strain harbo...

  4. Detection of Shiga toxin-producing Escherichia coli (STEC) O157:H7, O26, O45, O103, O111, O121, and O145, and Salmonella in retail raw ground beef using the DuPont BAX system

    Science.gov (United States)

    Shiga toxin-producing Escherichia coli (STEC) and Salmonella are food-borne pathogens commonly associated with beef, and reliable methods are needed to determine their prevalence in beef and to ensure food safety. Retail ground beef was tested for the presence of E. coli O157:H7, STEC serogroups O2...

  5. Genes ycfR, sirA and yigG contribute to the surface attachment of Salmonella enterica Typhimurium and Saintpaul to fresh produce.

    Directory of Open Access Journals (Sweden)

    Joelle K Salazar

    Full Text Available Salmonella enterica is a frequent contaminant of minimally-processed fresh produce linked to major foodborne disease outbreaks. The molecular mechanisms underlying the association of this enteric pathogen with fresh produce remain largely unexplored. In our recent study, we showed that the expression of a putative stress regulatory gene, ycfR, was significantly induced in S. enterica upon exposure to chlorine treatment, a common industrial practice for washing and decontaminating fresh produce during minimal processing. Two additional genes, sirA involved in S. enterica biofilm formation and yigG of unknown function, were also found to be differentially regulated under chlorine stress. To further characterize the roles of ycfR, sirA, and yigG in S. enterica attachment and survival on fresh produce, we constructed in-frame deletions of all three genes in two different S. enterica serovars, Typhimurium and Saintpaul, which have been implicated in previous disease outbreaks linked to fresh produce. Bacterial attachment to glass and polystyrene microtiter plates, cell aggregation and hydrophobicity, chlorine resistance, and surface attachment to intact spinach leaf and grape tomato were compared among wild-type strains, single-gene deletion mutants, and their respective complementation mutants. The results showed that deletions of ycfR, sirA, and yigG reduced bacterial attachment to glass and polystyrene as well as fresh produce surface with or without chlorine treatment in both Typhimurium and Saintpaul. Deletion of ycfR in Typhimurium significantly reduced bacterial chlorine resistance and the attachment to the plant surfaces after chlorinated water washes. Deletions of ycfR in Typhimurium and yigG in Saintpaul resulted in significant increase in cell aggregation. Our findings suggest that ycfR, sirA, and yigG collectively contribute to S. enterica surface attachment and survival during post-harvest minimal processing of fresh produce.

  6. Detection and Characterization of Shiga Toxin Producing Escherichia coli, Salmonella spp., and Yersinia Strains from Human, Animal, and Food Samples in San Luis, Argentina

    Directory of Open Access Journals (Sweden)

    Gabriela Isabel Favier

    2014-01-01

    Full Text Available Shiga toxin producing Escherichia coli (STEC, Salmonella spp., and Yersinia species was investigated in humans, animals, and foods in San Luis, Argentina. A total of 453 samples were analyzed by culture and PCR. The antimicrobial susceptibility of all the strains was studied, the genomic relationships among isolates of the same species were determined by PFGE, and the potencial virulence of Y. enterocolitica strains was analyzed. Yersinia species showed higher prevalence (9/453, 2.0%, 95% CI, 0.7–3.3% than STEC (4/453, 0.9%, 95% CI, 0–1.8% and Salmonella spp. (3/453, 0.7%, 95% CI, 0–1.5%. Y. enterocolitica and Y. intermedia were isolated from chicken carcasses (6/80, 7.5%, 95% CI, 1.5–13.5% and porcine skin and bones (3/10, 30%, 95% CI, 0–65%. One STEC strain was recovered from human feces (1/70, 1.4%, 95% CI, 0–4.2% and STEC stx1/stx2 genes were detected in bovine stools (3/129, 2.3%, 95% CI, 0–5.0%. S. Typhimurium was isolated from human feces (1/70, 1.4%, 95% CI, 0–4.2% while one S. Newport and two S. Gaminara strains were recovered from one wild boar (1/3, 33%, 95% CI, 0–99%. The knowledge of prevalence and characteristics of these enteropathogens in our region would allow public health services to take adequate preventive measures.

  7. Essential oils reduce Escherichia coli O157:H7 and Salmonella on iceberg and romaine lettuce without affecting produce quality

    Science.gov (United States)

    Foodborne outbreaks associated with the consumption of fresh produce have increased. In an effort to identify natural antimicrobial agents as fresh produce wash; the effect of essential oils in reducing enteric pathogens on iceberg and romaine lettuce was investigated. Cut lettuce pieces (3 x 2 cm) ...

  8. Salmonella Infections (For Parents)

    Science.gov (United States)

    ... Needs a Kidney Transplant Vision Facts and Myths Salmonella Infections KidsHealth > For Parents > Salmonella Infections Print A ... Last? Can Salmonella Infections Be Prevented? What Is Salmonella ? Salmonella is a kind of bacteria , with many ...

  9. Salmonella: Salmonellosis

    DEFF Research Database (Denmark)

    Löfström, Charlotta; Hansen, Trine; Maurischat, Sven

    2015-01-01

    Salmonella remains one of the most important zoonotic pathogenic bacteria and is the causative agents of salmonellosis. The aim of this article is to give an overview of Salmonella and salmonellosis, starting by describing the characteristics of the microorganism Salmonella, including biochemical...... properties, physiology, classification, and nomenclature. Thereafter, the epidemiology of the organism is introduced, including the routes of transmission. Finally, the disease salmonellosis, the virulence mechanisms, and the occurrence in different types of food are described....

  10. Standards to Make ESC Rights Justiciable: A Summary Exploration

    NARCIS (Netherlands)

    Ch. Courtis (Christian)

    2009-01-01

    textabstractSocial rights – or economic, social and cultural rights (ESC rights) – are not a new idea. There have been examples of the statutory recognition of ESC rights since the last third of the nineteenth century. ESC rights entered the language of constitutional law in the period between the

  11. 4,4′-Diaponeurosporene-Producing Bacillus subtilis Increased Mouse Resistance against Salmonella typhimurium Infection in a CD36-Dependent Manner

    Directory of Open Access Journals (Sweden)

    Qian Yang

    2017-04-01

    Full Text Available Deficient mucosal innate immunity is a hallmark of infectious diarrhea, such as Salmonella typhimurium (S. typhimurium-induced gastroenteritis. Here, we report that oral administration of a 4,4′-diaponeurosporene-producing Bacillus subtilis (B.s-Dia could improve mice mucosal immunity, as showed by an increased resistance against S. typhimurium infection. Intragastric administration of B.s-Dia for 7 days could increase the secretion of CCL20 by intestinal epithelial cells (IECs and then recruit more dendritic cells. Meanwhile, the number of CD8αα+ intraepithelial lymphocytes, which play a critical role in downregulating immune responses, was also reduced, probably as a consequence of the decrease of IEC-derived TGFβ. Further study showed that CD36 played a critical role in B.s-Dia-induced immune enhancement, as blocking CD36 signal with a specific antagonist, sulfo-N-succinimidyl oleate, led to the inability of B.s-Dia to enhance mucosal innate immunity.

  12. CTX-M extended-spectrum β-lactamase-producing Klebsiella spp, Salmonella spp, Shigella spp and Escherichia coli isolates in Iranian hospitals

    National Research Council Canada - National Science Library

    Bialvaei, Abed Zahedi; Kafil, Hossein Samadi; Asgharzadeh, Mohammad; Aghazadeh, Mohammad; Yousefi, Mehdi

    2016-01-01

    .... From January 2012 to December 2013, totally 198 E. coli, 139 Klebsiella spp, 54 Salmonella spp and 52 Shigella spp from seven hospitals of six provinces in Iran were screened for resistance to extended-spectrum cephalosporins...

  13. Influence of subinhibitory-concentration (sub-MIC Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi

    Directory of Open Access Journals (Sweden)

    Rahul Narasanna, Manjunath Chavadi, Ajaykumar Oli

    2017-06-01

    Full Text Available Objectives: In the present study, we have analyzed ESBL-producing S. typhi’s capability in forming a significant amount of biofilm on plastic and glass surface, and the influence of cefetoxime on biofilm development at subinhibitory (Sub-MIC concentration. Methods: Nine strains of cefetoxime-mediated ESBL-producing S. typhi were used in the study. S. typhi formed biofilm on plastic and glass materials; it was demonstrated using micro titre plate (MTP and standard test tube methods. Comparative study of the influence of cefetoxime on biofilm formation in its MIC (128 µg/ml and at sub-MIC (64 µg/ml was demonstrated by microtitre plate method. The biofilm production was observed in SEM images, statistical analysis (ANOVA showed significant increase in cell surface and volume due to the influence of Cefetoxime. Results: Of the nine selected isolates, two S. typhi strains, namely BST 51 and BST 130, produced relatively strong biofilm in the presence of cefetoxime at sub-MIC level (64 µg/ml, comparatively weak biofilm formation at MIC level (128 µg/ml. Typical morphological changes were observed in cefetoxime-resistant strains, S. typhi BST 51 and BST 130, in comparison to cefetoxime-sensitive strain S. typhi BST 63 used as a control. We found an increase in surface and volume of a cell in response to cefetoxime and statistical data (ANOVA proved that resistant strains were significantly different from control strains. Conclusion: The above study clearly shows that cefetoxime at sub-MIC level efficiently induces biofilm formation and promotes changes in morphology of the cell. J Microbiol Infect Dis 2017; 7(2: 67-75

  14. Estimating the probability and level of contamination with Salmonella of feed for finishing pigs produced in Switzerland--the impact of the production pathway.

    Science.gov (United States)

    Sauli, I; Danuser, J; Geeraerd, A H; Van Impe, J F; Rüfenacht, J; Bissig-Choisat, B; Wenk, C; Stärk, K D C

    2005-04-15

    Contaminated feed is a source of infection with Salmonella for livestock, including pigs. Because pigs rarely show clinical signs of salmonellosis, undetected carriers can enter the food production chain. In a "Farm to Fork" food safety concept, safe feed is the first step for ensuring safe food. Heat treatment or adding organic acids are process steps for reducing or eliminating a contamination with Salmonella. The aims of this study were (I) to estimate the probability and the level of Salmonella contamination in batches of feed for finishing pigs in Swiss mills and (II) to assess the efficacy of specific process steps for reducing the level of contamination with Salmonella. A quantitative release assessment was performed by gathering and combining data on the various parameters having an influence on the final contamination of feed. Fixed values and probability distributions attributed to these parameters were used as input values for a Monte Carlo simulation. The simulation showed that-depending on the production pathway-the probability that a batch of feed for finishing pigs contains Salmonella ranged from 34% (for feed on which no specific decontaminating step was applied) to 0% (for feed in which organic acids were added and a heat treatment was implemented). If contamination occurred, the level of contamination ranged from a few Salmonella kg(-1) feed to a maximum of 8E+04 Salmonella kg(-1) feed. Probability and levels of contamination were highest when no production process able to reduce or eliminate the pathogen was implemented. However, most of the Swiss production was shown to undergo some kind of decontaminating step. A heat treatment, in combination with the use of organic acids, was found as a solution of choice for the control of Salmonella in feed.

  15. Intrafamilial transmission of extended-spectrum-beta-lactamase-producing Escherichia coli and Salmonella enterica Babelsberg among the families of internationally adopted children.

    Science.gov (United States)

    Tandé, D; Boisramé-Gastrin, S; Münck, M R; Héry-Arnaud, G; Gouriou, S; Jallot, N; Nordmann, P; Naas, T

    2010-05-01

    International adoption from developing countries has become an increasing phenomenon in recent years. Given the high prevalence of multidrug-resistant (MDR) bacteria in these countries, the adopted children represent a group at risk for both carriage and infection with MDR bacteria. The dynamics of intrafamilial transmission of MDR bacteria after adoption was studied in a prospective study from January 2002 to January 2005. Stool samples, taken at the first visit to the outpatient adoption practice and subsequently every month, from the adopted children of an orphanage of Bamako (Mali) and from all the members of their adoptive families were screened for MDR bacteria and bacterial pathogens. Bacteria were characterized by standard biochemical methods, disc diffusion antibiograms, PFGE and plasmid analysis. beta-Lactamase genes were sought by PCR. Over the study period, 52 ESBL-producing Enterobacteriaceae (E-ESBL), with Escherichia coli (56%) being the most prevalent, were isolated from 24/25 adoptees at arrival in France. During follow-up, the transmission of ESBL-producing E. coli and Salmonella enterica Babelsberg between the adoptees and their adoptive family members has clearly been demonstrated for 5/22 families (23%). The mean duration of the carriage for the adopted children was 9 months (1-15 months). CTX-M-15 was the most prevalent resistance gene among the E-ESBLs (93%), while SHV-12 was found among the S. enterica Babelsberg studied. International travellers, transfer of patients and now adoption may contribute to the global emergence of MDR bacteria. Thus, in addition to the usual screening of adopted children for infectious diseases, additional screening for MDR bacteria should be recommended, at least for children coming from countries with a high prevalence of MDR bacteria.

  16. Mexican unpasteurised fresh cheeses are contaminated with Salmonella spp., non-O157 Shiga toxin producing Escherichia coli and potential uropathogenic E. coli strains: A public health risk.

    Science.gov (United States)

    Guzman-Hernandez, Rosa; Contreras-Rodriguez, Araceli; Hernandez-Velez, Rosa; Perez-Martinez, Iza; Lopez-Merino, Ahide; Zaidi, Mussaret B; Estrada-Garcia, Teresa

    2016-11-21

    Fresh cheeses are a main garnish of Mexican food. Consumption of artisanal fresh cheeses is very common and most of them are made from unpasteurised cow milk. A total of 52 fresh unpasteurised cheeses of five different types were purchased from a variety of suppliers from Tabasco, Mexico. Using the most probable number method, 67% and 63% of samples were positive for faecal coliforms and E. coli, respectively; revealing their low microbiological quality. General hygienic conditions and practices of traditional cheese manufacturers were poor; most establishments had unclean cement floors, all lacked windows and doors screens, and none of the food-handlers wore aprons, surgical masks or bouffant caps. After analysing all E. coli isolates (121 strains) for the presence of 26 virulence genes, results showed that 9 (17%) samples were contaminated with diarrheagenic E. coli strains, 8 harboured non-O157 Shiga toxin producing E. coli (STEC), and one sample contained both STEC and diffusely adherent E. coli strains. All STEC strains carried the stx1 gene. Potential uropathogenic E. coli (UPEC) strains were isolated from 15 (29%) samples; the most frequent gene combination was fimA-agn43. Two samples were contaminated with Salmonella. The results demonstrated that unpasteurised fresh cheeses produced in Tabasco are of poor microbiological quality and may frequently harbour foodborne pathogens. Food safety authorities in Mexico need to conduct more rigorous surveillance of fresh cheeses. Furthermore, simple and inexpensive measures as establishing programs emphasizing good hand milking practices and hygienic manufacturing procedures may have a major effect on improving the microbiological quality of these food items. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. SALMONELLA SPECIES

    African Journals Online (AJOL)

    DR. AMINU

    e. Biochemical screening and serological tests for Salmonellae. Identification of Salmonella species was done biochemically. Triple sugar Iron (TSI) agar motility, urease and citrate utilization tests were also used to screen the isolates before serologic testing was performed. (Cheesbrough, 2002; Perilla, 2003). Triple sugar ...

  18. Could ESC (Electronic Stability Control) change the way we drive?

    Science.gov (United States)

    Rudin-Brown, Christina M; Jenkins, Richard W; Whitehead, Tanya; Burns, Peter C

    2009-08-01

    ESC (Electronic Stability Control) is a crash avoidance technology that reduces the likelihood of collisions involving loss of control. Although past and emerging research indicates that ESC is effective in reducing collision rates and saving lives, and its inclusion in all vehicle platforms is encouraged, drivers may demonstrate behavioral adaptation or an overreliance on ESC that could offset or reduce its overall effectiveness. The main objective of the present study was to determine whether behavioral adaptation to ESC is likely to occur upon the widespread introduction of ESC into the Canadian vehicle fleet. Secondary objectives were to confirm the results of a previous ESC public survey and to generate a baseline measure for the future assessment of planned and ongoing ESC promotional activities in Canada. Two separate telephone surveys evaluated drivers' perceptions and awareness of ESC. The first surveyed 500 randomly selected owners/drivers of passenger vehicles. The second surveyed 1017 owners/drivers of 2006-2008 ESC-equipped passenger vehicles from the provinces of Quebec and British Columbia, Canada. Though ESC drivers were much more likely than drivers of other vehicles to be aware of ESC (77% vs. 39%) and that their own vehicle was equipped with it (63% vs. 8%), 23 percent had never heard of it. Ninety percent of drivers who knew that their vehicle was equipped with ESC believed that ESC had made it safer to drive and reported being confident that ESC would work in an emergency. Twenty-three percent of ESC owners who knew their vehicle had ESC reported noticing long-lasting changes in their driving behavior since they began driving the vehicle. Collectively, results suggest that behavioral adaptation to ESC is likely in certain drivers; however, its proven effectiveness in reducing the likelihood of being involved in a serious crash probably outweighs any potential increases in unsafe driving. To fully benefit from ESC, vehicle manufacturers are

  19. Detection of blaCTX-M Extended Spectrum Beta-lactamase Producing Salmonella enterica Serotype Typhi in a Tertiary Care Centre.

    Science.gov (United States)

    Ramachandran, Aishwarya; Shanthi, Mariappan; Sekar, Uma

    2017-09-01

    Infections caused by Salmonella are an important public health threat in tropical and subtropical countries. Due to the emergence of resistance to ampicillin, chloramphenicol and trimethoprim/sulfamethoxazole (multidrug resistant salmonellae) in the late 1980s, fluoroquinolones and extended spectrum cephalosporins became the drugs of choice. Resistance to cefotaxime and ceftriaxone due to the production of Extended Spectrum Beta-Lactamase (ESBL) and reduced susceptibility to ciprofloxacin have emerged resulting in treatment failure. The Cefotaximase (CTX-M) type ESBLs are the most widespread beta lactamase among Enterobacteriaceae including salmonellae. To detect the presence of blaCTX-M in salmonellae causing human infections. Detection of qnr genes to identify the coexistence of blaCTX-M and qnr gene. The study included 103 consecutive, non-repetitive salmonellae isolated from clinical specimens obtained from July 2015- June 2016 which were identified up to species level by conventional/automated methods. Susceptibility to various classes of antimicrobial agents was determined by disc diffusion method. Minimum Inhibitory Concentration (MIC) to cefotaxime and ceftriaxone was determined by agar dilution method. The results were interpreted in accordance with Clinical & Laboratory Standard Institute (CLSI) (guidelines 2015. Detection of the ESBL phenotype was performed by the combined disk method. Polymerase Chain Reaction (PCR) amplification of all isolates was performed using group specific primers to characterize the presence of blaCTX-M, qnrA, qnrB and qnrS. Of the 103 study isolates two isolates of Salmonella typhi were resistant to cefotaxime and ceftriaxone and had a MIC of 128μg/ml. PCR amplification and sequencing detected the presence of blaCTX-M-15 in these two isolates. These two isolates exhibited resistance to ciprofloxacin in vitro but qnr gene was not detected in these isolates. Resistance to third generation cephalosporins among salmonellae is a

  20. Purified galactooligosaccharide, derived from a mixture produced by the enzymic activity of Bifidobacterium bifidum, reduces Salmonella enterica serovar Typhimurium adhesion and invasion in vitro and in vivo.

    Science.gov (United States)

    Searle, Laura E J; Cooley, William A; Jones, Gareth; Nunez, Alejandro; Crudgington, Bentley; Weyer, Ute; Dugdale, Alexandra H; Tzortzis, George; Collins, James W; Woodward, Martin J; La Ragione, Roberto M

    2010-12-01

    The prebiotic Bimuno(®) is a mixture containing galactooligosaccharides (GOSs), produced by the galactosyltransferase activity of Bifidobacterium bifidum NCIMB 41171 using lactose as the substrate. Previous in vivo and in vitro studies demonstrating the efficacy of Bimuno(®) in reducing Salmonella enterica serovar Typhimurium (S. Typhimurium) colonization did not ascertain whether or not the protective effects could be attributed to the prebiotic component GOS. Here we wished to test the hypothesis that GOS, derived from Bimuno(®), may confer the direct anti-invasive and protective effects of Bimuno(®). In this study the efficacy of Bimuno(®), a basal solution of Bimuno(®) without GOS [which contained glucose, galactose, lactose, maltodextrin and gum arabic in the same relative proportions (w/w) as they are found in Bimuno(®)] and purified GOS to reduce S. Typhimurium adhesion and invasion was assessed using a series of in vitro and in vivo models. The novel use of three dimensionally cultured HT-29-16E cells to study prebiotics in vitro demonstrated that the presence of ∼ 5 mg Bimuno(®) ml(-1) or ∼ 2.5 mg GOS ml(-1) significantly reduced the invasion of S. Typhimurium (SL1344nal(r)) (PBimuno(®) or GOS prevented the adherence or invasion of S. Typhimurium to enterocytes, and thus reduced its associated pathology. This protection appeared to correlate with significant reductions in the neutral and acidic mucins detected in goblet cells, possibly as a consequence of stimulating the cells to secrete the mucin into the lumen. In all assays, Bimuno(®) without GOS conferred no such protection, indicating that the basal solution confers no protective effects against S. Typhimurium. Collectively, the studies presented here clearly indicate that the protective effects conferred by Bimuno(®) can be attributed to GOS.

  1. Microarray-based detection of antibiotic resistance and virulence factor genes in Salmonella spp. isolated from food-producing animals and processed food

    OpenAIRE

    Figueiredo, Rui Filipe Ramos

    2016-01-01

    Tese de doutoramento em Ciências Farmacêuticas, na especialidade de Microbiologia e Parasitologia, apresentada à Faculdade de Farmácia da Universidade de Coimbra Salmonella enterica é uma bactéria patogénica de origem alimentar que infecta seres humanos pelo mundo inteiro. Nalguns casos, as infeções por Salmonella requerem tratamento com antibióticos. A resistência a agentes antimicrobianos é um problema global e leva ao insucesso do tratamento de infeções bacterianas. Alguns estudos têm s...

  2. Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs

    Directory of Open Access Journals (Sweden)

    Choo Andre

    2011-04-01

    Full Text Available Abstract Background Exosomes or secreted bi-lipid vesicles from human ESC-derived mesenchymal stem cells (hESC-MSCs have been shown to reduce myocardial ischemia/reperfusion injury in animal models. However, as hESC-MSCs are not infinitely expansible, large scale production of these exosomes would require replenishment of hESC-MSC through derivation from hESCs and incur recurring costs for testing and validation of each new batch. Our aim was therefore to investigate if MYC immortalization of hESC-MSC would circumvent this constraint without compromising the production of therapeutically efficacious exosomes. Methods The hESC-MSCs were transfected by lentivirus carrying a MYC gene. The transformed cells were analyzed for MYC transgene integration, transcript and protein levels, and surface markers, rate of cell cycling, telomerase activity, karyotype, genome-wide gene expression and differentiation potential. The exosomes were isolated by HPLC fractionation and tested in a mouse model of myocardial ischemia/reperfusion injury, and infarct sizes were further assessed by using Evans' blue dye injection and TTC staining. Results MYC-transformed MSCs largely resembled the parental hESC-MSCs with major differences being reduced plastic adherence, faster growth, failure to senesce, increased MYC protein expression, and loss of in vitro adipogenic potential that technically rendered the transformed cells as non-MSCs. Unexpectedly, exosomes from MYC-transformed MSCs were able to reduce relative infarct size in a mouse model of myocardial ischemia/reperfusion injury indicating that the capacity for producing therapeutic exosomes was preserved. Conclusion Our results demonstrated that MYC transformation is a practical strategy in ensuring an infinite supply of cells for the production of exosomes in the milligram range as either therapeutic agents or delivery vehicles. In addition, the increased proliferative rate by MYC transformation reduces the time

  3. Effect of kosher processing on Shiga Toxin producing Escherichia coli (STEC) and Salmonella on surfaces of fresh beef and its quality.

    Science.gov (United States)

    Introduction: The main reason that people buy kosher products is for the impression of improved food quality and safety. However, both STEC and Salmonella may contaminate surfaces of fresh beef during slaughtering and many antimicrobial interventions cannot be applied due to kosher restrictions. Al...

  4. Spread of Extended Spectrum Cephalosporinase-Producing Escherichia coli Clones and Plasmids from Parent Animals to Broilers and to Broiler Meat in a Production Without Use of Cephalosporins

    DEFF Research Database (Denmark)

    Agersø, Yvonne; Jensen, Jacob Dyring; Hasman, Henrik

    2014-01-01

    selects for ESC-producing E. coli and whether certain clones or plasmids spread from imported parent flocks to the meat. Materials and Methods: ESC-producing E. coli was isolated using MacConkey broth with 1 mg/L of ceftriaxone. ESC genes were identified using polymerase chain reaction and sequencing...... of ESC E. coli. Conclusions: ESC-producing E. coli from flocks of imported broiler parents spread clonally and horizontally to broiler meat (including potentially human pathogenic types) even in a country with no cephalosporin use. Use of aminopenicillins may influence the persistence of ESC-producing E....... coli in the broiler production, but other factors should be investigated....

  5. Ansiedad escénica y flamenco

    OpenAIRE

    Mariola Lupiáñez Castillo; Rafael Hoces Ortega.

    2016-01-01

    Cuando el artista flamenco sube al escenario, además de expresar su arte y disfrutar con su ejecución, es también objeto de la valoración de su público. Cuando los pensamientos y la atención del artista se centran solo en este escrutinio de la audiencia se puede desarrollar miedo o incomodidad a la hora de actuar, padeciendo lo que se denomina ansiedad escénica. Esta fobia social aparece cuando la persona teme ser evaluada negativamente por los demás y mostrar síntomas de nerviosismo que pued...

  6. Prevention of Salmonella contamination of finished soybean meal used for animal feed by a Norwegian production plant despite frequent Salmonella contamination of raw soy beans, 1994–2012

    OpenAIRE

    Wierup, Martin; Kristoffersen, Thor

    2014-01-01

    Background Salmonella contaminated animal feed is a major source for introducing Salmonella into the animal derived food chain. Because soybeans frequently are contaminated with Salmonella, soybean meal used as animal feed material, a by-product of a “crushing plant” which produces oil from soybeans, can be important source of Salmonella in the animal feed. We report the successful control of Salmonella from 1994 to 2012 in a Norwegian crushing plant producing soybean meal from imported soy b...

  7. Detection and characterization of extended-spectrum β-lactamases (blaCTX-M-1 and blaSHV producing Escherichia coli, Salmonella spp. and Klebsiella pneumoniae isolated from humans in Mizoram

    Directory of Open Access Journals (Sweden)

    Iadarilin Warjri

    2015-05-01

    Full Text Available Aim: The present study was conducted to isolate and characterize the extended spectrum β-lactamases (ESBLs producing enteric bacteria in human beings in Mizoram, India. Materials and Methods: Fecal samples were collected from human beings with or without the history of diarrhea from different hospitals of Mizoram. Samples were processed for isolation and identification of Escherichia coli, Salmonella and Klebsiella pneumoniae. All the isolates were subjected to antibiotic sensitivity assays. Phenotypically, ESBLs production ability was determined by double discs synergy test (DDST method. ESBLs producing isolates were subjected to polymerase chain reaction (PCR for detection of ESBLs genes. Plasmids were cured by acridine orange. Transfer of resistance from a donor to recipient strains was done by in vitro horizontal method. Results: A total of 414 enteric bacteria were isolated from 180 fecal samples (113 were from diarrheic patients and 67 were from non-diarrheic patients, of which 333 (80.44%, 52 (12.56%, and 29 (7.00% were E. coli, K. pneumoniae and Salmonella spp., respectively. Double discs synergy test (DDST exhibited 72 (21.62% E. coli, 12 (23.08% K. pneumoniae and 4 (13.79% Salmonella spp. were ESBLs producers. Altogether, 24 (13.04% isolates were found to be positive for at least one resistance genes under this study. A total of 36 (8.70% E. coli, 4 (0.97% K. pneumoniae and 2 (0.48% Salmonella spp. were found to be positive for blaCTX-M-1 gene by PCR. Similarly, 5 (1.21% E. coli and 4 (0.97% K. pneumoniae isolates were found to be positive for blaSHV gene. A total of 3 (0.72% K. pneumoniae isolates were recorded as positive for both blaCTX-M-1 and blaSHV genes. All the isolates were carrying plasmids ranging between 0.9 kb and ~30 kb. The resistance plasmid could not be transferred to a recipient by in vitro horizontal gene transfer method. Conclusion: ESBLs producing enteric bacteria are circulating in human population in North

  8. European Society of Cardiology (ESC) congress report from Amsterdam 2013.

    Science.gov (United States)

    Matsumoto, Yasuharu

    2013-01-01

    The Annual Congress of the European Society of Cardiology (ESC) was held in Amsterdam from the 31(st) of August to the 4(th) of September 2013. The total attendance up to the 3(rd) was 29,990. Several important topics were presented and discussed, including a randomized trial of preventive angioplasty in myocardial infarction (PRAMI), transcatheter aortic valve implantation, renal denervation, management of vasospastic angina, plaque vulnerability and outcome assessed by OCT and diuretic resistance in heart failure (an ESC-JCS [Japanese Circulation Society] joint session), and OCT-guided percutaneous coronary intervention. The ESC congress has become one of the most important and attractive international scientific meetings. Importantly, Japan topped the number of abstracts submitted (1,459 abstracts) and accepted (565 abstracts) to ESC 2013. Thus, the ESC is eager to continue the strong collaboration with the JCS as the relationship between them gets closer year by year.

  9. Live attenuated vaccines for invasive Salmonella infections

    Science.gov (United States)

    Tennant, Sharon M.; Levine, Myron M.

    2015-01-01

    Salmonella enterica serovar Typhi produces significant morbidity and mortality worldwide despite the fact that there are licensed S. Typhi vaccines available. This is primarily due to the fact that these vaccines are not used in the countries that most need them. There is growing recognition that an effective invasive Salmonella vaccine formulation must also prevent infection due to other Salmonella serovars. We anticipate that a multivalent vaccine that targets the following serovars will be needed to control invasive Salmonella infections worldwide: S. Typhi, S. Paratyphi A, S. Paratyphi B (currently uncommon but may become dominant again), S. Typhimurium, S. Enteritidis and S. Choleraesuis (as well as other Group C Salmonella). Live attenuated vaccines are an attractive vaccine formulation for use in developing as well as developed countries. Here, we describe the methods of attenuation that have been used to date to create live attenuated Salmonella vaccines and provide an update on the progress that has been made on these vaccines. PMID:25902362

  10. Ansiedad escénica y flamenco

    Directory of Open Access Journals (Sweden)

    Mariola Lupiáñez Castillo

    2016-01-01

    Full Text Available Cuando el artista flamenco sube al escenario, además de expresar su arte y disfrutar con su ejecución, es también objeto de la valoración de su público. Cuando los pensamientos y la atención del artista se centran solo en este escrutinio de la audiencia se puede desarrollar miedo o incomodidad a la hora de actuar, padeciendo lo que se denomina ansiedad escénica. Esta fobia social aparece cuando la persona teme ser evaluada negativamente por los demás y mostrar síntomas de nerviosismo que puedan ser perceptibles por el público. Las manifestaciones de la ansiedad se pueden clasificar en tres categorías: Cognitivas, fisiológicas y conductuales. Estas manifestaciones afectan al estado de ánimo, dificultan la ejecución de la tarea y predisponen al artista a evitar enfrentarse a futuras actuaciones. No controlar estas manifestaciones de la forma adecuada puede suponer en el músico o bailaor el detrimento de su calidad artística y en numerosas ocasiones, el abandono de la carrera artística. Estudios anteriores revelan que la formación académica y el contexto socio cultural en el que se desenvuelve el artista pueden incidir en el desarrollo y permanencia de la ansiedad escénica. Teniendo en cuenta las peculiaridades históricas y sociales del Flamenco así como la evolución de su formación, que ha pasado de la transmisión oral de un ambiente informal al estudio dentro de los conservatorios, se considera necesario estudiar cómo afecta la ansiedad a los artistas de este género para poder prevenir e intervenir en los músicos que la padezcan.

  11. Evaluation of gaseous chlorine dioxide as a sanitizer for killing Salmonella, Escherichia coli O157:H7, Listeria monocytogenes, and yeasts and molds on fresh and fresh-cut produce.

    Science.gov (United States)

    Sy, Kaye V; Murray, Melinda B; Harrison, M David; Beuchat, Larry R

    2005-06-01

    Gaseous chlorine dioxide (ClO2) was evaluated for effectiveness in killing Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes on fresh-cut lettuce, cabbage, and carrot and Salmonella, yeasts, and molds on apples, peaches. tomatoes, and onions. Inoculum (100 microl, ca. 6.8 log CFU) containing five serotypes of Salmonella enterica, five strains of E. coli O157:H7, or five strains of L. monocytogenes was deposited on the skin and cut surfaces of fresh-cut vegetables, dried for 30 min at 22 degrees C, held for 20 h at 4 degrees C, and then incubated for 30 min at 22 degrees C before treatment. The skin surfaces of apples, peaches, tomatoes, and onions were inoculated with 100 microl of a cell suspension (ca. 8.0 log CFU) containing five serotypes of Salmonella, and inoculated produce was allowed to dry for 20 to 22 h at 22 degrees C before treatment. Treatment with ClO2 at 4.1 mg/liter significantly (alpha = 0.05) reduced the population of foodborne pathogens on all produce. Reductions resulting from this treatment were 3.13 to 4.42 log CFU/g for fresh-cut cabbage, 5.15 to 5.88 log CFU/g for fresh-cut carrots, 1.53 to 1.58 log CFU/g for fresh-cut lettuce, 4.21 log CFU per apple, 4.33 log CFU per tomato, 1.94 log CFU per onion, and 3.23 log CFU per peach. The highest reductions in yeast and mold populations resulting from the same treatment were 1.68 log CFU per apple and 2.65 log CFU per peach. Populations of yeasts and molds on tomatoes and onions were not significantly reduced by treatment with 4.1 mg/liter ClO2. Substantial reductions in populations of pathogens on apples, tomatoes, and onions but not peaches or fresh-cut cabbage, carrot, and lettuce were achieved by treatment with gaseous ClO2 without markedly adverse effects on sensory qualities.

  12. European Society of Cardiology (ESC) Congress Report from Munich 2012.

    Science.gov (United States)

    Ozaki, Yukio

    2012-01-01

    The Annual Congress of the European Society of Cardiology (ESC) was held in Munich from the 26(th) to 29(th) of August 2012. The daily attendance ranged from 26,600 to 27,407 up to the 28(th) and several important issues were presented and discussed, including antiplatelet therapy for acute coronary syndrome (TRILOGY ACS), transcatheter aortic valve implantation, renal denervation, novel oral anticoagulants for atrial fibrillation (AFib), AFib ablation, the impact of the Great East Japan Earthquake on cardiovascular disease, management of vasospastic angina, plaque rupture and erosion (ESC-JCS [Japanese Circulation Society] joint session), heart failure, and FFR-guided percutaneous coronary intervention outcome. Three ESC "GOLD MEDALS" were awarded, including one to Professor Ryozo Nagai, the first Asian to receive this award. The ESC meeting has become one of the most important for updating not only general cardiologists' education but also specialists' expertise. Japan topped the number of abstracts submitted to ESC 2012 (>1,200 abstracts), while the ESC would like to establish a strong collaboration with the Japanese Cardiology Society. Relations between ESC and JCS will become closer and more favorable year by year.

  13. Chronological Change of Resistance to β-Lactams in Salmonella enterica serovar Infantis Isolated from Broilers in Japan.

    Science.gov (United States)

    Chuma, Takehisa; Miyasako, Daisuke; Dahshan, Hesham; Takayama, Tomoko; Nakamoto, Yuko; Shahada, Francis; Akiba, Masato; Okamoto, Karoku

    2013-01-01

    Epidemiologic surveillance study was conducted in southern Japan to determine the antimicrobial resistance phenotypes and characterize the β-lactamase genes and the plasmids harboring these genes in Salmonella enterica serovar Infantis (S. Infantis) isolates from broilers. Between January, 2007 and December, 2008, a total of 1,472 fecal samples were collected and examined at the Laboratory of Veterinary Public Health, Kagoshima University, Japan. In 93 (6.3%) isolates recovered, 33 (35.5%) isolates showed resistance to cefotaxime, an extended-spectrum cephalosporin (ESC), conferred by TEM-20, TEM-52 and CTX-M-25 extended-spectrum β-lactamases (ESBLs). In addition to ESC-resistance, eight (8.6%) isolates exhibited resistance to cefoxitin mediated by CMY-2 AmpC β-lactamase. Plasmid analysis and polymerase chain reaction replicon typing revealed the bla TEM-20 and bla CMY-2 genes were associated with IncP plasmids, bla TEM-52 was linked with a non-typable plasmid and bla CTX-M-25 was carried by an IncA/C plasmid. Non-β-lactam resistance to streptomycin, sulfamethoxazole, and oxytetracycline encoded by the aadA1, sul1, and tet(A) genes, respectively, was found in 86 (92.5%) isolates. Resistance to kanamycin and ofloxacin was exhibited in 12 (12.9%) and 11 (11.8%) isolates, respectively, the former was mediated by aphA1-Iab. These data indicate that S. Infantis isolates producing ESBLs and AmpC β-lactamase have spread among broiler farms in Japan. These data demonstrated that the incidence of ESC-resistant S. Infantis carrying bla TEM-52 remarkably increased and S. Infantis strains harboring bla CMY-2, bla TEM-20, or bla CTX-M-25 genes emerged from broilers in Japan for the first time in 2007 and 2008.

  14. Characterization of Salmonella enterica and detection of the virulence genes specific to diarrheagenic Escherichia coli from poultry carcasses in Ouagadougou, Burkina Faso.

    Science.gov (United States)

    Kagambèga, Assèta; Barro, Nicolas; Traoré, Alfred S; Siitonen, Anja; Haukka, Kaisa

    2012-07-01

    One hundred chicken carcasses purchased from three markets selling poultry in Ouagadougou, Burkina Faso, between June 2010 and October 2010 were examined for their microbiological quality. The presence of Salmonella was investigated using standard bacteriological procedures, and the isolates obtained were serotyped and tested for antimicrobial susceptibility. The presence of virulence-associated genes of the five main pathogroups of diarrheagenic Escherichia coli-Shiga toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli, and enteroinvasive E. coli-was investigated using 16-plex polymerase chain reaction (PCR) on the mixed bacterial cultures from the poultry samples. Of the 100 chicken carcasses studied, 57 were contaminated by Salmonella; 16 different serotypes were identified, the most frequent being Salmonella Derby, found in 28 samples. Four Salmonella strains were resistant to tetracycline, and two were resistant to streptomycin. Based on the PCR detection of the virulence genes, in total, 45 carcasses were contaminated by three pathogroups of E. coli: STEC, EPEC, or EAEC. The STEC and EPEC virulence genes were detected on six and 39 carcasses, respectively. EAEC virulence genes were only detected in combination with those of EPEC (on 11 carcasses) or STEC (on two carcasses). The STEC-positive carcasses contained the genes stx(1), stx(2), eaeA, escV, and ent in different combinations. None of the EPEC-positive carcasses contained the bfp gene, indicating that only atypical EPEC was present. EAEC virulence genes detected were aggR and/or pic. The high proportion of chicken carcasses contaminated by Salmonella and diarrheagenic E. coli indicates a potential food safety risk for consumers and highlights the necessity of public awareness of these pathogens.

  15. Prevalence of Shiga toxin Producing Escherichia coli, Salmonella enterica and Listeria monocytogenes at Public Access Watershed Sites in a California Central Coast Agricultural Region

    Directory of Open Access Journals (Sweden)

    Michael B Cooley

    2014-03-01

    Full Text Available Produce contaminated with enteric pathogens is a major source of foodborne illness in the United States. Lakes, streams, rivers, and ponds were sampled with Moore swabs bi-monthly for over two years at 30 locations in the vicinity of a leafy green growing region on the Central California Coast and screened for Shiga toxin producing Escherichia coli (STEC, Salmonella enterica, and Listeria monocytogenes to evaluate the prevalence and persistence of pathogen subtypes. The prevalence of STEC from 1,386 samples was 11%; 110 samples (8% contained E. coli O157:H7 with the highest prevalence occurring close to cattle operations. Non-O157 STEC isolates represented major clinical O-types and 57% contained both shiga toxin types 1 and 2 and intimin. Multiple Locus Variable Number Tandem Repeat Analysis of STEC isolates indicated prevalent strains during the period of study. Notably, Salmonella was present at high levels throughout the sampling region with 65% prevalence in 1,405 samples resulting in 996 isolates with slightly lower prevalence in late autumn. There were 2, 8 and 14 sites that were Salmonella-positive over 90%, 80% and 70% of the time, respectively. The serotypes identified most often were 6,8:d:-, Typhimurium, and Give. Interestingly, analysis by Pulsed Field Gel Electrophoresis indicated persistence and transport of pulsotypes in the region over several years. In this original study of L. monocytogenes in the region prevalence was 43% of 1,405 samples resulting in 635 individual isolates. Over 85% of the isolates belonged to serotype 4b with serotypes 1/2a, 1/2b, 3a, 4d with 4e representing the rest, and there were 12 and 2 sites that were positive over 50% and 80% of the time, respectively. Although surface water is not directly used for irrigation in this region, transport to the produce can occur by other means. This environmental survey assesses initial contamination levels towards an understanding of transport leading to produce

  16. A multi-country Salmonella Enteritidis phage type 14b outbreak associated with eggs from a German producer: 'near real-time' application of whole genome sequencing and food chain investigations, United Kingdom, May to September 2014.

    Science.gov (United States)

    Inns, T; Lane, C; Peters, T; Dallman, T; Chatt, C; McFarland, N; Crook, P; Bishop, T; Edge, J; Hawker, J; Elson, R; Neal, K; Adak, G K; Cleary, P

    2015-04-23

    We report an outbreak of Salmonella Enteritidis phage type 14b (PT14b) in the United Kingdom (UK) between May and September 2014 where Public Health England launched an investigation to identify the source of infection and implement control measures. During the same period, outbreaks caused by a Salmonella Enteritidis strain with a specific multilocus variable-number tandem repeat analysis (MLVA) profile occurred in other European Union Member States. Isolates from a number of persons affected by the UK outbreak, who had initially been tested by MLVA also shared this particular profile. Cases were defined as any person infected with S. Enteritidis PT14b, resident in England or Wales and without history of travel outside of this geographical area during the incubation period, reported from 1 June 2014 onwards, with a MLVA profile of 2–11–9-7–4-3–2-8–9 or a single locus variant thereof. In total, 287 cases met the definition. Food traceback investigations in the UK and other affected European countries linked the outbreaks to chicken eggs from a German company. We undertook whole genome sequencing of isolates from UK and European cases, implicated UK premises, and German eggs: isolates were highly similar. Combined with food traceback information, this confirmed that the UK outbreak was also linked to a German producer.

  17. Source attribution of human Salmonella cases in Sweden

    DEFF Research Database (Denmark)

    Wahlström, H.; Andersson, Y.; Plym-Forshell, L.

    2010-01-01

    The aim of this study was to identify the sources of sporadic domestic Salmonella cases in Sweden and to evaluate the usefulness of a source-attribution model in a country in which food animals are virtually free from Salmonella. The model allocates human sporadic domestic Salmonella cases...... to different sources according to distribution of Salmonella subtypes in the different sources. Sporadic domestic human Salmonella cases (n=1086) reported between July 2004 and June 2006 were attributed to nine food-animal and wildlife sources. Of all Salmonella cases, 82% were acquired abroad and 2.9% were...... associated with outbreaks. We estimated that 6.4% were associated with imported food, 0.5% with food-producing animals, and 0.6% with wildlife. Overall, 7.7% could not be attributed to any source. We concluded that domestic food-producing animals are not an important source for Salmonella in humans in Sweden...

  18. Assessment of Salmonella survival in dry-cured Italian salami.

    Science.gov (United States)

    Bonardi, S; Bruini, I; Bolzoni, L; Cozzolino, P; Pierantoni, M; Brindani, F; Bellotti, P; Renzi, M; Pongolini, S

    2017-12-04

    The inactivation of Salmonella during curing of Italian traditional pork salami was investigated. A total of 150 batches of ground raw meat (GRM) used for salami manufacturing by four producers were tested for Salmonella by real-time PCR followed by ISO 6579 cultural confirmation and MPN enumeration. Salami produced with Salmonella positive GRMs were re-tested at the end of their curing period. Aw, pH and NaCl content were also measured. Detection of Salmonella was performed testing both 25 and 50g of the samples. By Real-Time PCR 37% of the GRMs resulted positive, but cultural detection of Salmonella was obtained in 14% of the samples only. Salmonella enumeration ranged from 31 MPN/g to Salmonella in 100% of all positive samples, vs. 62% of ISO-25g. Salami made of the contaminated GRMs were 29% Salmonella-positive, as most batches of salami produced with Salmonella-positive GRMs resulted negative after regular curing (20-48days). Overall, 13% of salami produced with Salmonella-contaminated GRMs were positive. They belonged to six batches, which turned out negative after prolonged curing ranging between 49 and 86days. Salmonella enumeration in salami ranged from 8.7 MPN/g to Salmonella in cured salami (p value: >0.05). The most common Salmonella serovars in GRMs were Derby (52%), Typhimurium monophasic variant 4, (Barbuti et al., 1993), 12:i:- (19%) and Stanley (10%). Salmonella Derby (56%), London, Branderup, Panama (13%, respectively) and Goldcoast (6%) were most frequent in cured salami. The study showed negative correlation between real-time CT values and cultural confirmation of Salmonella, as well as the importance of sample size for Salmonella detection. Among considered factors with possible effect on the occurrence of Salmonella in salami, statistical analysis revealed a role for aw in salami and for Salmonella load in GRMs, while pH and NaCl content did not significantly affect the probability of finding Salmonella in dry-cured salami in the context of

  19. Inconsistencies in the development of the ESC Clinical Practice Guidelines for Heart Failure.

    Science.gov (United States)

    Coats, Andrew J Stewart; Shewan, Louise G

    2013-10-03

    The publication of the European Society of Cardiology (ESC) guidelines for the management of heart failure, in 2012 represented the latest and arguably the most comprehensive document to date summarising recommended treatment and diagnostic options for the care of heart failure patients. The impact of clinical practice guidelines is now so great that it is important to review the processes that underlie guideline development. The ESC guideline process is compared and contrasted to those of other guideline bodies. The ESC uses its own internal experts inclined to review source clinical trial data rather than published or commissioned meta-analyses and systematic reviews. Uncertainties exist in several areas, such as how are the scope of potential treatments to be reviewed chosen, if there is no call for proposals or external consultation?, Two illustrative discrepancies are highlighted i) the non-surgical MitraClip device for reducing mitral regurgitation is given the verbal equivalent of a Class IIb recommendation on the basis of 107 patients in an uncontrolled registry, whereas no drug is reviewed based on such data, and another device, the subject of 3 prospective randomised controlled trials, was not reviewed at all and ii) for Ivabradine the whole trial population was included in the recommendation, despite a subgroup not benefitting, whereas for CRT the sub-group not thought to benefit was excluded from the recommendation. We propose that more interaction is needed between ESC and stakeholders so each can better understand the processes for producing guidelines to improve some of these aspects. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  20. Esc4 and the Control of Recombination During Replication

    Science.gov (United States)

    Chin, Jodie K.; Bashkirov, Vladimir I.; Heyer, Wolf-Dietrich; Romesberg, Floyd E.

    2010-01-01

    When replication forks stall during DNA synthesis, cells respond by assembling multi-protein complexes to control the various pathways that stabilize the replication machinery, repair the replication fork, and facilitate the reinitiation of processive DNA synthesis. Increasing evidence suggests that cells have evolved scaffolding proteins to orchestrate and control the assembly of these repair complexes, typified in mammalian cells by several BRCT-motif containing proteins, such as Brca1, Xrcc1, and 53BP1. In Saccharomyces cerevisiae, Esc4 contains six such BRCT domains and is required for the most efficient response to a variety of agents that damage DNA. We show that Esc4 interacts with several proteins involved in the repair and processing of stalled or collapsed replication forks, including the recombination protein Rad55. However, the function of Esc4 does not appear to be restricted to a Rad55-dependent process, as we observed an increase in sensitivity to the DNA alkylating agent methane methylsulfonate (MMS) in a esc4Δ rad55Δ mutant, as well as in double mutants of esc4Δ and other recombination genes, compared to the corresponding single mutants. In addition, we show that Esc4 forms multiple nuclear foci in response to treatment with MMS. Similar behavior is also observed in the absence of damage when either of the S-phase checkpoint proteins, Tof1 or Mrc1, is deleted. Thus, we propose that Esc4 associates with ssDNA of stalled forks and acts as a scaffolding protein to recruit and/or modulate the function of other proteins required to reinitiate DNA synthesis. PMID:16569515

  1. Efficacy of a Blend of Sulfuric Acid and Sodium Sulfate against Shiga Toxin-Producing Escherichia coli, Salmonella, and Nonpathogenic Escherichia coli Biotype I on Inoculated Prerigor Beef Surface Tissue.

    Science.gov (United States)

    Scott-Bullard, Britteny R; Geornaras, Ifigenia; Delmore, Robert J; Woerner, Dale R; Reagan, James O; Morgan, J Bred; Belk, Keith E

    2017-12-01

    A study was conducted to investigate the efficacy of a sulfuric acid-sodium sulfate blend (SSS) against Escherichia coli O157:H7, non-O157 Shiga toxin-producing E. coli (STEC), Salmonella, and nonpathogenic E. coli biotype I on prerigor beef surface tissue. The suitability of using the nonpathogenic E. coli as a surrogate for in-plant validation studies was also determined by comparing the data obtained for the nonpathogenic inoculum with those for the pathogenic inocula. Prerigor beef tissue samples (10 by 10 cm) were inoculated (ca. 6 log CFU/cm 2 ) on the adipose side in a laboratory-scale spray cabinet with multistrain mixtures of E. coli O157:H7 (5 strains), non-O157 STEC (12 strains), Salmonella (6 strains), or E. coli biotype I (5 strains). Treatment parameters evaluated were two SSS pH values (1.5 and 1.0) and two spray application pressures (13 and 22 lb/in 2 ). Untreated inoculated beef tissue samples served as controls for initial bacterial populations. Overall, the SSS treatments lowered inoculated (6.1 to 6.4 log CFU/cm 2 ) bacterial populations by 0.6 to 1.5 log CFU/cm 2 (P coli inocula; however, solution pH did have a significant effect (P coli inoculum to the SSS treatments was similar (P ≥ 0.05) to that of the pathogenic inocula tested, making the E. coli biotype I strains viable surrogate organisms for in-plant validation of SSS efficacy on beef. The application of SSS at the tested parameters to prerigor beef surface tissue may be an effective intervention for controlling pathogens in a commercial beef harvest process.

  2. Efficacy of Neutral pH Electrolyzed Water in Reducing Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 on Fresh Produce Items using an Automated Washer at Simulated Food Service Conditions.

    Science.gov (United States)

    Afari, George K; Hung, Yen-Con; King, Christopher H

    2015-08-01

    The objective of this study was to determine the efficacy of neutral pH electrolyzed (NEO) water (155 mg/L free chlorine, pH 7.5) in reducing Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 on romaine lettuce, iceberg lettuce, and tomatoes washed in an automated produce washer for different times and washing speeds. Tomatoes and lettuce leaves were spot inoculated with 100 μL of a 5 strain cocktail mixture of either pathogen and washed with 10 or 8 L of NEO water, respectively. Washing lettuce for 30 min at 65 rpm led to the greatest reductions, with 4.2 and 5.9 log CFU/g reductions achieved for E. coli O157:H7 and S. Typhimurium respectively on romaine, whereas iceberg lettuce reductions were 3.2 and 4.6 log CFU/g for E. coli O157:H7 and S. Typhimurium respectively. Washing tomatoes for 10 min at 65 rpm achieved reductions greater than 8 and 6 log CFU/tomato on S. Typhimurium and E. coli O157:H7 respectively. All pathogens were completely inactivated in NEO water wash solutions. No detrimental effects on the visual quality of the produce studied were observed under all treatment conditions. Results show the adoption of this washing procedure in food service operations could be useful in ensuring produce safety. © 2015 Institute of Food Technologists®

  3. Effects of antimicrobials fed as dietary growth promoters on faecal shedding of Campylobacter, Salmonella and shiga-toxin producing Escherichia coli in swine.

    Science.gov (United States)

    Wells, J E; Kalchayanand, N; Berry, E D; Oliver, W T

    2013-02-01

    To determine whether antimicrobials commonly used in swine diets affect zoonotic pathogen shedding in faeces. Barrows (n = 160) were sorted into two treatments at 10 weeks of age (week 0 of the study), and fed growing, grow finishing and finishing diets in 4-week feeding periods. For each feeding phase, diets were prepared without (A-) and with (A+) dietary antimicrobials (chlortetracycline, 0-8 week; bacitracin, 9-12 week) typical of the United States. At week 0, 4, 8, 9, 10 and 12 of the study, faecal swabs or grabs were collected for analyses. Campylobacter spp. was absent at week 0, but prevalence increased over time with most isolates being identified as Campylobacter coli. When chlortetracycline was used in A+ diets (week 4 and 8), prevalence for Campylobacter spp., pathogenic Escherichia coli O26 and stx genes was lower in faeces. On week 12 after the shift to bacitracin, Campylobacter spp. and stx genes were higher in faeces from piglets fed A+ diet. Pathogenic E. coli serogroups O103 and O145 were isolated throughout the study and their prevalence did not differ due to diet. Pathogenic E. coli serogroups O111 and O121 were never found in the piglets, and Salmonella spp. prevalence was low. In production swine, growing diets with chlortetracycline may have reduced pathogen shedding compared with the A-growing diets, whereas finishing diets with bacitracin may have increased pathogen shedding compared with the A-finishing diet. Inclusion of antimicrobials in the diet can affect zoonotic pathogen shedding in faeces of swine. © 2012 No claim to US Government works.

  4. European Society of Cardiology (ESC) congress report from Barcelona 2014.

    Science.gov (United States)

    Muramatsu, Takashi; Ozaki, Yukio

    2014-01-01

    The Annual Congress of the European Society of Cardiology (ESC) was held in Barcelona from 30th August to 3rd September 2014. More than 30,300 attendees from around the world shared the latest original research, including 27 clinical Hot Line studies, 12 basic science Hot Lines, 15 clinical trial updates, 19 registry studies, and 4,597 abstracts. Many important issues were presented, including novel treatment strategies for heart failure, acute coronary syndrome, interventional treatment for structural heart disease, renal denervation, novel anticoagulant therapies, atrial fibrillation and so on. In addition, 5 new ESC clinical practice guidelines (ie, myocardial revascularization, non-cardiac surgery, acute pulmonary embolism, hypertrophic cardiomyopathy, and aortic disease) were launched. It should be noted that Japan has recently been ranked in the top position in terms of the number of abstract submissions. Based on these activities, the ESC Congress has been recognized as the dominant scientific and educational forum for healthcare professionals in cardiology. We report the highlights and several key presentations of the ESC Congress 2014. The scientific activities and growing contributions of Japanese cardiologists or cardiovascular surgeons enhance the favorable relationship between the ESC and the Japanese Circulation Society.

  5. Epigenetic landscaping during hESC differentiation to neural cells.

    Science.gov (United States)

    Golebiewska, Anna; Atkinson, Stuart P; Lako, Majlinda; Armstrong, Lyle

    2009-06-01

    The molecular mechanisms underlying pluripotency and lineage specification from embryonic stem cells (ESCs) are still largely unclear. To address the role of chromatin structure in maintenance of pluripotency in human ESCs (hESCs) and establishment of lineage commitment, we analyzed a panel of histone modifications at promoter sequences of genes involved in maintenance of pluripotency, self-renewal, and in early stages of differentiation. To understand the changes occurring at lineage-specific gene regulatory sequences, we have established an efficient purification system that permits the examination of two distinct populations of lineage committed cells; fluorescence activated cell sorted CD133(+) CD45(-)CD34(-) neural stem cells and beta-III-tubulin(+) putative neurons. Here we report the importance of other permissive marks supporting trimethylation of Lysine 4 H3 at the active stem cell promoters as well as poised bivalent and nonbivalent lineage-specific gene promoters in hESCs. Methylation of lysine 9 H3 was found to play a role in repression of pluripotency-associated and lineage-specific genes on differentiation. Moreover, presence of newly formed bivalent domains was observed at the neural progenitor stage. However, they differ significantly from the bivalent domains observed in hESCs, with a possible role of dimethylation of lysine 9 H3 in repressing the poised genes.

  6. An assessment of soybeans and other vegetable proteins as source of salmonella contamination in pig production

    OpenAIRE

    Häggblom Per; Wierup Martin

    2010-01-01

    Abstract Background The impact of salmonella contaminated feed ingredients on the risk for spreading salmonella to pigs was assessed in response to two incidences when salmonella was spread by feed from two feed mills to 78 swine producing herds. Methods The assessment was based on results from the salmonella surveillance of feed ingredients before introduction to feed mills and from HACCP - based surveillance of the feed mills. Results from the mills of the Company (A) that produced the salm...

  7. Salmonella Diagnosis and Treatment

    Science.gov (United States)

    ... FDA) USDA Food Safety and Inspection Service Follow Salmonella RSS Diagnosis and Treatment Recommend on Facebook Tweet Share Compartir How Can Salmonella Infections Be Diagnosed? Diagnosing salmonellosis requires testing a ...

  8. Occurrence of Salmonella sp in laying hens

    Directory of Open Access Journals (Sweden)

    Gama NMSQ

    2003-01-01

    Full Text Available This study was carried out to investigate the presence of Salmonella sp in flocks of white laying hens. In different farms, the transport boxes of twelve flocks were inspected at arrival for the presence of Salmonella. Four positive (A, B, L and M and one negative (I flocks were monitored at each four weeks using bacteriological examination of cecal fresh feces up to 52 weeks. Birds were also evaluated at 52 weeks, when 500 eggs were taken randomly, and at 76 weeks, after forced molt. Salmonella enterica serovar Enteritidis and S. enterica rough strain were isolated from the transport boxes of the four positive flocks (flocks A, B, L and M. Salmonella sp was not isolated from the transport boxes or from the feces after 76 weeks-old in flock I. Salmonella sp was isolated in the 1st, 11th, 34th, 42nd and 76th weeks from flock A; in the 1st, 4th, 11th and 76th weeks from flock B; in the first week and in the 17th to 52nd weeks from flock L; and in the 1st and 76th weeks from flock M. S. Enteritidis, S. enterica rough strain and Salmonella enterica serovar Infantis were isolated from the four positive flocks. Besides, Salmonella enterica serovar Javiana was isolated from flocks B and L, and Salmonella enterica serovar Mbandaka was isolated from flock L. Eggs produced by flock A and by flock L were contaminated with S. Enteritidis and S. enterica rough strain. According to these results, Salmonella-infected flocks may produce contaminated eggs.

  9. Salmonella in Swedish cattle

    OpenAIRE

    Ågren, Estelle

    2017-01-01

    In Sweden, all herds detected with salmonella are put under restrictions and measures aiming at eradication are required. The purpose of these studies was to provide a basis for decisions on how surveillance and control of salmonella in Swedish cattle can be made more cost-efficient. Results from a bulk milk screening were used to investigate seroprevalence of salmonella and to study associations between salmonella status and geographical location, local animal density, number of test pos...

  10. 78 FR 42526 - Salmonella

    Science.gov (United States)

    2013-07-16

    ... HUMAN SERVICES Food and Drug Administration Salmonella Contamination of Dry Dog Food; Withdrawal of...) entitled ``Sec. 690.700 Salmonella Contamination of Dry Dog Food.'' This CPG is obsolete. DATES: The.... SUPPLEMENTARY INFORMATION: FDA issued the CGP entitled ``Sec. 690.700 Salmonella Contamination of Dry Dog Food...

  11. Interactions of Salmonella enterica with lettuce leaves.

    Science.gov (United States)

    Kroupitski, Y; Pinto, R; Brandl, M T; Belausov, E; Sela, S

    2009-06-01

    To investigate the interactions of Salmonella enterica with abiotic and plant surfaces and their effect on the tolerance of the pathogen to various stressors. Salmonella strains were tested for their ability to form biofilm in various growth media using a polystyrene plate model. Strong biofilm producers were found to attach better to intact Romaine lettuce leaf tissue compared to weak producers. Confocal microscopy and viable count studies revealed preferential attachment of Salmonella to cut-regions of the leaf after 2 h at 25 degrees C, but not for 18 h at 4 degrees C. Storage of intact lettuce pieces contaminated with Salmonella for 9 days at 4 degrees C resulted only in small changes in population size. Exposure of lettuce-associated Salmonella cells to acidic conditions (pH 3.0) revealed increased tolerance of the attached vs planktonic bacteria. Biofilm formation on polystyrene may provide a suitable model to predict the initial interaction of Salmonella with cut Romaine lettuce leaves. Association of the pathogen with lettuce leaves facilitates its persistence during storage and enhances its acid tolerance. Understanding the interactions between foodborne pathogens and lettuce might be useful in developing new approaches to prevent fresh produce-associated outbreaks.

  12. The hESC line Envy expresses high levels of GFP in all differentiated progeny.

    Science.gov (United States)

    Costa, Magdaline; Dottori, Mirella; Ng, Elizabeth; Hawes, Susan M; Sourris, Koula; Jamshidi, Pegah; Pera, Martin F; Elefanty, Andrew G; Stanley, Edouard G

    2005-04-01

    Human embryonic stem cells (hESCs) have been advanced as a potential source of cells for use in cell replacement therapies. The ability to identify hESCs and their differentiated progeny readily in transplantation experiments will facilitate the analysis of hESC potential and function in vivo. We have generated a hESC line designated 'Envy', in which robust levels of green fluorescent protein (GFP) are expressed in stem cells and all differentiated progeny.

  13. File list: Pol.PSC.50.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.mESCs,_differentiated mm9 RNA polymerase Pluripotent stem cell mESCs, different...iated SRX590276 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.50.AllAg.mESCs,_differentiated.bed ...

  14. File list: ALL.PSC.20.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.20.AllAg.mESCs,_differentiated mm9 All antigens Pluripotent stem cell mESCs, different...barchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.20.AllAg.mESCs,_differentiated.bed ...

  15. File list: DNS.PSC.20.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.mESCs,_differentiated mm9 DNase-seq Pluripotent stem cell mESCs, different...iated http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.20.AllAg.mESCs,_differentiated.bed ...

  16. File list: ALL.PSC.05.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.mESCs,_differentiated mm9 All antigens Pluripotent stem cell mESCs, different...barchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.05.AllAg.mESCs,_differentiated.bed ...

  17. File list: ALL.PSC.50.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.50.AllAg.mESCs,_differentiated mm9 All antigens Pluripotent stem cell mESCs, different...barchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.50.AllAg.mESCs,_differentiated.bed ...

  18. File list: Pol.PSC.05.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.mESCs,_differentiated mm9 RNA polymerase Pluripotent stem cell mESCs, different...iated SRX590276 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.05.AllAg.mESCs,_differentiated.bed ...

  19. File list: Oth.PSC.05.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.05.AllAg.mESCs,_differentiated mm9 TFs and others Pluripotent stem cell mESCs, different...8,SRX209322,SRX065538,SRX065537,SRX523453,SRX523451 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.05.AllAg.mESCs,_differentiated.bed ...

  20. File list: Oth.PSC.10.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.AllAg.mESCs,_differentiated mm9 TFs and others Pluripotent stem cell mESCs, different...2,SRX065538,SRX523453,SRX754570,SRX065537,SRX523451 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.10.AllAg.mESCs,_differentiated.bed ...

  1. File list: Unc.PSC.05.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.mESCs,_differentiated mm9 Unclassified Pluripotent stem cell mESCs, different...iated http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.05.AllAg.mESCs,_differentiated.bed ...

  2. File list: DNS.PSC.50.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.50.AllAg.mESCs,_differentiated mm9 DNase-seq Pluripotent stem cell mESCs, different...iated http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.50.AllAg.mESCs,_differentiated.bed ...

  3. File list: Pol.PSC.20.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.mESCs,_differentiated mm9 RNA polymerase Pluripotent stem cell mESCs, different...iated SRX590276 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.20.AllAg.mESCs,_differentiated.bed ...

  4. File list: Pol.PSC.10.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.mESCs,_differentiated mm9 RNA polymerase Pluripotent stem cell mESCs, different...iated SRX590276 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.10.AllAg.mESCs,_differentiated.bed ...

  5. File list: Unc.PSC.20.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.20.AllAg.mESCs,_differentiated mm9 Unclassified Pluripotent stem cell mESCs, different...iated http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.20.AllAg.mESCs,_differentiated.bed ...

  6. File list: ALL.PSC.10.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.mESCs,_differentiated mm9 All antigens Pluripotent stem cell mESCs, different...barchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.10.AllAg.mESCs,_differentiated.bed ...

  7. File list: Unc.PSC.10.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.10.AllAg.mESCs,_differentiated mm9 Unclassified Pluripotent stem cell mESCs, different...iated http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.10.AllAg.mESCs,_differentiated.bed ...

  8. File list: Oth.PSC.20.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.AllAg.mESCs,_differentiated mm9 TFs and others Pluripotent stem cell mESCs, different...4,SRX754570,SRX065538,SRX523453,SRX523451,SRX065537 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.AllAg.mESCs,_differentiated.bed ...

  9. File list: Oth.PSC.50.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.50.AllAg.mESCs,_differentiated mm9 TFs and others Pluripotent stem cell mESCs, different...8,SRX523453,SRX523451,SRX754570,SRX882858,SRX065537 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.50.AllAg.mESCs,_differentiated.bed ...

  10. File list: Unc.PSC.50.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.50.AllAg.mESCs,_differentiated mm9 Unclassified Pluripotent stem cell mESCs, different...iated http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.50.AllAg.mESCs,_differentiated.bed ...

  11. File list: DNS.PSC.05.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.mESCs,_differentiated mm9 DNase-seq Pluripotent stem cell mESCs, different...iated http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.05.AllAg.mESCs,_differentiated.bed ...

  12. File list: DNS.PSC.10.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.mESCs,_differentiated mm9 DNase-seq Pluripotent stem cell mESCs, different...iated http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.10.AllAg.mESCs,_differentiated.bed ...

  13. 75 FR 18849 - Small Entity Compliance Guide: Prevention of Salmonella Enteritidis in Shell Eggs During...

    Science.gov (United States)

    2010-04-13

    ... HUMAN SERVICES Food and Drug Administration Small Entity Compliance Guide: Prevention of Salmonella... availability of a guidance entitled ``Prevention of Salmonella Enteritidis in Shell Eggs During Production... producers to implement measures to prevent Salmonella Enteritidis (SE) from contaminating eggs on the farm...

  14. Isolation of QseC-regulated genes in Salmonella enterica serovar Typhimurium by transposon mutgagenesis

    Science.gov (United States)

    Non-typhoidal Salmonella, a leading cause of U.S. foodborne disease and food-related deaths, often asymptomatically colonizes food-producing animals. In fact, >50% of U.S. swine production facilities test positive for Salmonella. The multidrug-resistant (MDR) Salmonella Typhimurium DT104 NCTC13348 c...

  15. Incentive systems for food quality control with repeated deliveries: Salmonella control in pork production

    NARCIS (Netherlands)

    King, R.P.; Backus, G.B.C.; Gaag, van der M.A.

    2007-01-01

    This paper presents a dynamic principal-agent analysis of incentive systems for Salmonella control. The European Union will require Salmonella testing from 2008. On the basis of the producer's performance history in controlling Salmonella, the incentive systems analysed determine quality premiums to

  16. Isolation and characterization of lactobacillus and bacillus producing ...

    African Journals Online (AJOL)

    The biosurfactants were investigated for potential antimicrobial activity using disk diffusion method against causal organisms of food borne illnesses. These food borne pathogens include Salmonella typhi, Escherichia coli, Staphylococcus aureus, Salmonella paratyphi and Shigella dysentrae. Biosurfactant producing ...

  17. Persistence of salmonella typhimurium in nopal cladodes

    Science.gov (United States)

    Fresh produce associated outbreaks have increased in the last few years. E.coli O157:H7 and Salmonella have been causative agents of infection in these outbreaks. Fresh produce is consumed raw, and in the absence of terminal kill treatment, it is imperative to understand sources of contamination o...

  18. Geographical distribution of salmonella infected pig, cattle and sheep herds in Sweden 1993-2010

    OpenAIRE

    Skog Lars; Lewerin Susanna; Frössling Jenny; Wahlström Helene

    2011-01-01

    Abstract Background The Swedish salmonella control programme covers the entire production chain, from feed to food. All salmonella serotypes are notifiable. On average, less than 20 cases of salmonella in food-producing animals are reported every year. In some situations, the cases would be expected to cluster geographically. The aim of this study was to illustrate the geographic distribution of the salmonella cases detected in pigs, cattle and sheep. Methods Data on all herds with pigs, catt...

  19. Inactivation of Salmonella Senftenberg, Salmonella Typhimurium and Salmonella Tennessee in peanut butter by 915 MHz microwave heating.

    Science.gov (United States)

    Song, Won-Jae; Kang, Dong-Hyun

    2016-02-01

    This study evaluated the efficacy of a 915 MHz microwave with 3 different levels to inactivate 3 serovars of Salmonella in peanut butter. Peanut butter inoculated with Salmonella enterica serovar Senftenberg, S. enterica serovar Typhimurium and S. enterica serovar Tennessee were treated with a 915 MHz microwave with 2, 4 and 6 kW and acid and peroxide values and color changes were determined after 5 min of microwave heating. Salmonella populations were reduced with increasing treatment time and treatment power. Six kW 915 MHz microwave treatment for 5 min reduced these three Salmonella serovars by 3.24-4.26 log CFU/g. Four and two kW 915 MHz microwave processing for 5 min reduced these Salmonella serovars by 1.14-1.48 and 0.15-0.42 log CFU/g, respectively. Microwave treatment did not affect acid, peroxide, or color values of peanut butter. These results demonstrate that 915 MHz microwave processing can be used as a control method for reducing Salmonella in peanut butter without producing quality deterioration. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Prevention of Salmonella contamination of finished soybean meal used for animal feed by a Norwegian production plant despite frequent Salmonella contamination of raw soy beans, 1994–2012

    Science.gov (United States)

    2014-01-01

    Background Salmonella contaminated animal feed is a major source for introducing Salmonella into the animal derived food chain. Because soybeans frequently are contaminated with Salmonella, soybean meal used as animal feed material, a by-product of a “crushing plant” which produces oil from soybeans, can be important source of Salmonella in the animal feed. We report the successful control of Salmonella from 1994 to 2012 in a Norwegian crushing plant producing soybean meal from imported soy beans. The results are based on an officially supervised HACCP based program including annual testing of around 4000 samples. Results During the 19-year period, 34% of samples collected during unloading of ships delivering soybeans yielded Salmonella; the proportion of samples from ships that yielded Salmonella varied from 12-62% each year. Dust samples from all shiploads from South America yielded Salmonella. In total 94 serovars of Salmonella were isolated, including nine (90%) of the EU 2012 top ten serovars isolated from clinical cases of salmonellosis in humans, including major animal pathogenic serovars like Spp. Typhimurium and Enteritidis. The effectiveness of the HACCP based control was indicated by a low prevalence of Salmonella contamination in the clean area of the plant, which is considered to be the main reason for the successful prevention of Salmonella in the end product. Despite extensive testing, no sample from the finished soybean meal product was found to be Salmonella contaminated. Conclusions This study shows that a HAACP-based control program in a soybean crushing plant can produce Salmonella free soybean meal despite frequent Salmonella contamination of raw soybeans. That approach is suggested as an effective way to minimize the risk of Salmonella exposure of the animal feed mills and contamination of the subsequent animal feed chain. PMID:25011553

  1. Prevalence of Nontyphoidal Salmonella and Salmonella Strains with Conjugative Antimicrobial-Resistant Serovars Contaminating Animal Feed in Texas.

    Science.gov (United States)

    Hsieh, Yi-Cheng; Poole, Toni L; Runyon, Mick; Hume, Michael; Herrman, Timothy J

    2016-02-01

    The objective of this study was to characterize 365 nontyphoidal Salmonella enterica isolates from animal feed. Among the 365 isolates, 78 serovars were identified. Twenty-four isolates (7.0%) were recovered from three of six medicated feed types. Three of these isolates derived from the medicated feed, Salmonella Newport, Salmonella Typhimurium var. O 5- (Copenhagen), and Salmonella Lexington var. 15+ (Manila), displayed antimicrobial resistance. Susceptibility testing revealed that only 3.0% (12) of the 365 isolates displayed resistance to any of the antimicrobial agents. These 12 isolates were recovered from unmedicated dry beef feed (n = 3), medicated dry beef feed (n = 3), cabbage culls (n = 2), animal protein products (n = 2), dry dairy cattle feed (n = 1), and fish meal (n = 1). Only Salmonella Newport and Salmonella Typhimurium var. O 5- (Copenhagen) were multidrug resistant. Both isolates possessed the IncA/C replicon and the blaCMY-2 gene associated with cephalosporin resistance. Plasmid replicons were amplified from 4 of 12 resistant isolates. Plasmids (40 kb) were Salmonella Montevideo and Salmonella Kentucky. Conjugation experiments were done using 7 of the 12 resistant isolates as donors. Only Salmonella Montevideo, possessing a plasmid and amplifying IncN, produced transconjugants. Transconjugants displayed the same antimicrobial resistance profile as did the donor isolate. Three isolates that amplified replicons corresponding to IncA/C or IncHI2 did not produce transconjugants at 30 or 37°C. The results of this study suggest that the prevalence of antimicrobial-resistant Salmonella contaminating animal feed is low in Texas. However, Salmonella was more prevalent in feed by-products; fish meal had the highest prevalence (84%) followed by animal protein products (48%). Ten of the 35 feed types had no Salmonella contamination. Further investigation is needed to understand the possible role of specific feed types in the dissemination of antimicrobial

  2. A modular and flexible ESC-based mouse model of pancreatic cancer.

    Science.gov (United States)

    Saborowski, Michael; Saborowski, Anna; Morris, John P; Bosbach, Benedikt; Dow, Lukas E; Pelletier, Jerry; Klimstra, David S; Lowe, Scott W

    2014-01-01

    Genetically engineered mouse models (GEMMs) have greatly expanded our knowledge of pancreatic ductal adenocarcinoma (PDAC) and serve as a critical tool to identify and evaluate new treatment strategies. However, the cost and time required to generate conventional pancreatic cancer GEMMs limits their use for investigating novel genetic interactions in tumor development and maintenance. To address this problem, we developed flexible embryonic stem cell (ESC)-based GEMMs that facilitate the rapid generation of genetically defined multiallelic chimeric mice without further strain intercrossing. The ESCs harbor a latent Kras mutant (a nearly ubiquitous feature of pancreatic cancer), a homing cassette, and other genetic elements needed for rapid insertion and conditional expression of tetracycline-controlled transgenes, including fluorescence-coupled shRNAs capable of efficiently silencing gene function by RNAi. This system produces a disease that recapitulates the progression of pancreatic cancer in human patients and enables the study and visualization of the impact of gene perturbation at any stage of pancreas cancer progression. We describe the use of this approach to dissect temporal roles for the tumor suppressor Pten and the oncogene c-Myc in pancreatic cancer development and maintenance.

  3. El escándalo Volskwagen. Caso de estudio

    OpenAIRE

    Ruiz de Alba, José Luis; López-Toro, Alberto Antonio; Pastor García, María Inmaculada; Plaza Angulo, Juan José; Sarria Lozano, Enrique

    2017-01-01

    El vídeo se encuadra en el Proyecto de Innovación Educativa PIE15/147 de la Universidad de Málaga, dirigido por el profesor Alberto A. López Toro y realizado por el Centro de Tratamiento de la Imagen de la UMA. Se trata de un estudio de caso basado en el escándolo Volskwagen y se aplica también el aprendizaje basado en problemas y proyectos.

  4. Application of multiple locus variable number of tandem repeat analysis (MLVA), phage typing and antimicrobial susceptibility testing to subtype Salmonella enterica serovar Typhimurium isolated from pig farms, pork slaughterhouses and meat producing plants in Ireland.

    Science.gov (United States)

    Prendergast, D M; O'Grady, D; Fanning, S; Cormican, M; Delappe, N; Egan, J; Mannion, C; Fanning, J; Gutierrez, M

    2011-08-01

    Salmonella enterica subsp. enterica serovar Typhimurium is a common zoonotic pathogen encountered in Irish pigs and the pork industry and its characterisation using highly discriminatory typing methods is necessary for epidemiological studies, outbreak investigation and control. Multiple locus variable number of tandem repeat analysis (MLVA), phage typing and antimicrobial susceptibility testing were applied to characterise 301 S. typhimurium isolates of porcine origin isolated from farms, slaughterhouses and pork meat producing plants in Ireland over a four-year period. 154 MLVA patterns were obtained compared to 19 phage types and 38 AMR patterns, and MLVA was particularly useful for discriminating isolates of the same phage type, e.g. DT104 and DT104b, or isolates that were Untypable or in the category of "react with phage but does not conform to a recognised phage type" (RDNC) by the phage typing method. Cluster analysis of MLVA profiles using a minimum spanning tree (MST) demonstrated two major clusters (I and II), which showed to have a clear association with phage types, cluster I associated to phage types DT104, U302 and DT120 and cluster II associated to DT193 and U288. The results of this present study showed that MLVA is highly discriminatory and permitted the identification of identical profiles among isolates obtained at different points of the pork food chain. The same MLVA profile was observed in some cases among isolates with different phage types. While this can be explained by the fact that some phage types are closely related, it also indicates that combining phage typing and MLVA enhances strain typing of S. typhimurium. Copyright © 2011 Elsevier Ltd. All rights reserved.

  5. Effect of ROCK inhibitor Y-27632 on normal and variant human embryonic stem cells (hESCs) in vitro: its benefits in hESC expansion.

    Science.gov (United States)

    Gauthaman, Kalamegam; Fong, Chui-Yee; Bongso, Ariff

    2010-03-01

    The Rho associated coiled coil protein kinase (ROCK) dependent signaling pathway plays an important role in numerous physiological functions such as cell proliferation, adhesion, migration and inflammation. Human embryonic stem cells (hESCs) undergo differentiation and poor survival after single cell dissociation in culture thus limiting their expansion for cell based therapies. We evaluated the role of the selective ROCK inhibitor Y-27632 on hESC colonies and disassociated single hESCs from two different hESC lines. Karyotypically normal hESCs (HES3) and variant hESCs (BG01V) were treated with Y-27632 at 5, 10 and 20 muM concentrations for 72 h and its effects on hESC self renewal, colony morphology, cell cycle and pluripotency were evaluated. Increased cell proliferation of both HES3 and BG01V were observed for all three concentrations compared to untreated controls following passaging of cell clusters or dissociated single cells and some of these increases were statistically significant. Cell cycle assay demonstrated normal cell cycle progression with no peaks evident of apoptosis. No morphological differentiation was evident following treatment with the highest concentration of Y-27632 (20 muM) and the stemness related genes continued to be highly expressed in both HES3 and BG01V cells compared to untreated controls. The results confirmed that Y-27632 is a useful agent that aids in the expansion of undifferentiated hESC numbers for downstream applications in regenerative medicine.

  6. Prevention of Salmonella contamination of finished soybean meal used for animal feed by a Norwegian production plant despite frequent Salmonella contamination of raw soy beans, 1994-2012.

    Science.gov (United States)

    Wierup, Martin; Kristoffersen, Thor

    2014-07-11

    Salmonella contaminated animal feed is a major source for introducing Salmonella into the animal derived food chain. Because soybeans frequently are contaminated with Salmonella, soybean meal used as animal feed material, a by-product of a "crushing plant" which produces oil from soybeans, can be important source of Salmonella in the animal feed. During the 19-year period, 34% of samples collected during unloading of ships delivering soybeans yielded Salmonella; the proportion of samples from ships that yielded Salmonella varied from 12-62% each year. Dust samples from all shiploads from South America yielded Salmonella. In total 94 serovars of Salmonella were isolated, including nine (90%) of the EU 2012 top ten serovars isolated from clinical cases of salmonellosis in humans, including major animal pathogenic serovars like Spp. Typhimurium and Enteritidis. This study shows that a HAACP-based control program in a soybean crushing plant can produce Salmonella free soybean meal despite frequent Salmonella contamination of raw soybeans. That approach is suggested as an effective way to minimize the risk of Salmonella exposure of the animal feed mills and contamination of the subsequent animal feed chain.

  7. Differences in attachment of Salmonella enterica serovars to cabbage and lettuce leaves.

    Science.gov (United States)

    Patel, Jitendra; Sharma, Manan

    2010-04-30

    This study investigated the ability of five Salmonella enterica serovars to attach to and colonize intact and cut lettuce (Iceberg, Romaine) and cabbage surfaces. Biofilm formation and attachment of Salmonella serovars to intact and cut leaves were determined. Populations of loosely and strongly attached Salmonella were obtained to calculate the attachment strength (S(R)). Biofilm formation, as determined by microtiter plate assay, varied with strain and growth medium used. Salmonella Tennessee and S. Thompson produced stronger biofilms compared to S. Newport, S. Negev, and S. Braenderup. Biofilm formation was also stronger when Salmonella spp. were grown in diluted TSB (1:10). S. Tennessee, which produced strong biofilms, attached to produce surfaces at significantly higher numbers than the populations of S. Negev. Overall, S. Tennessee displayed more biofilm formation in vitro and attached more strongly to lettuce than other serovars. All Salmonella serovars attached rapidly on intact and cut produce surfaces. Salmonella spp. attached to Romaine lettuce at significantly higher numbers than those attached to Iceberg lettuce or cabbage. Salmonella attached preferentially to cut surface of all produce; however, the difference between Salmonella populations attached to intact and cut surfaces was similar (P>0.05). Salmonella attachment to both intact and cut produce surfaces increased with time. The overall attachment strength of Salmonella was significantly lower on cabbage (0.12) followed by Iceberg (0.23) and Romaine lettuce (0.34). Cabbage, intact or cut, did not support attachment of Salmonella as well as Romaine lettuce. Understanding the attachment mechanisms of Salmonella to produce may be useful in developing new intervention strategies to prevent produce outbreaks. Published by Elsevier B.V.

  8. Salmonella Sepsis in African Children

    African Journals Online (AJOL)

    Infection with both Salmonella typhiand non-typhi salmonella. (NTS) is common among children in many African countries. Salmonella typhi predominates among older children and adults with the typical localising features of enteric fever. Nontyphoid salmonellae species are more often reported among children under 5 ...

  9. Genomics of Salmonella Species

    Science.gov (United States)

    Canals, Rocio; McClelland, Michael; Santiviago, Carlos A.; Andrews-Polymenis, Helene

    Progress in the study of Salmonella survival, colonization, and virulence has increased rapidly with the advent of complete genome sequencing and higher capacity assays for transcriptomic and proteomic analysis. Although many of these techniques have yet to be used to directly assay Salmonella growth on foods, these assays are currently in use to determine Salmonella factors necessary for growth in animal models including livestock animals and in in vitro conditions that mimic many different environments. As sequencing of the Salmonella genome and microarray analysis have revolutionized genomics and transcriptomics of salmonellae over the last decade, so are new high-throughput sequencing technologies currently accelerating the pace of our studies and allowing us to approach complex problems that were not previously experimentally tractable.

  10. Human ESCs predisposition to karyotypic instability: Is a matter of culture adaptation or differential vulnerability among hESC lines due to inherent properties?

    Directory of Open Access Journals (Sweden)

    Bueno Clara

    2008-10-01

    Full Text Available Abstract Background The use of human embryonic stem cells (hESCs in research is increasing and hESCs hold the promise for many biological, clinical and toxicological studies. Human ESCs are expected to be chromosomally stable since karyotypic changes represent a pitfall for potential future applications. Recently, several studies have analysed the genomic stability of several hESC lines maintained after prolonged in vitro culture but controversial data has been reported. Here, we prompted to compare the chromosomal stability of three hESC lines maintained in the same laboratory using identical culture conditions and passaging methods. Results Molecular cytogenetic analyses performed in three different hESC lines maintained in parallel in identical culture conditions revealed significant differences among them in regard to their chromosomal integrity. In feeders, the HS181, SHEF-1 and SHEF-3 hESC lines were chromosomally stable up to 185 passages using either mechanical or enzymatic dissection methods. Despite the three hESC lines were maintained under identical conditions, each hESC line behaved differently upon being transferred to a feeder-free culture system. The two younger hESC lines, HS181 (71 passages and SHEF-3 (51 passages became chromosomally unstable shortly after being cultured in feeder-free conditions. The HS181 line gained a chromosome 12 by passage 17 and a marker by passage 21, characterized as a gain of chromosome 20 by SKY. Importantly, the mosaicism for trisomy 12 gradually increased up to 89% by passage 30, suggesting that this karyotypic abnormality provides a selective advantage. Similarly, the SHEF-3 line also acquired a trisomy of chromosome 14 as early as passage 10. However, this karyotypic aberration did not confer selective advantage to the genetically abnormal cells within the bulk culture and the level of mosaicism for the trisomy 14 remained overtime between 15%–36%. Strikingly, however, a much older hESC line

  11. Poor biofilm-forming ability and long-term survival of invasive Salmonella Typhimurium ST313.

    Science.gov (United States)

    Ramachandran, Girish; Aheto, Komi; Shirtliff, Mark E; Tennant, Sharon M

    2016-07-01

    Salmonella enterica serovar Typhimurium, an enteric pathogen that causes a self-limiting gastroenteritis, forms biofilms on different surfaces. In sub-Saharan Africa, Salmonella Typhimurium of a novel sequence type (ST) 313 was identified and produces septicemia in the absence of gastroenteritis. No animal reservoir has been identified, and it is hypothesized that transmission occurs via human to human. In this study, we show that invasive Salmonella Typhimurium ST313 strains from Mali are poor biofilm producers compared to Salmonella Typhimurium ST19 strains, which are found worldwide and are known to be associated with gastroenteritis. We evaluated biofilms using crystal violet staining, examination of the red, dry and rough morphotype, pellicle formation and a continuous flow system. One month-old Salmonella Typhimurium ST19 colonies survived in the absence of exogenous nutrients and were highly resistant to sodium hypochlorite treatment compared to Salmonella Typhimurium ST313. This study for the first time demonstrates the comparative biofilm-forming ability and long-term survival of clinical Salmonella Typhimurium ST19 and ST313 isolates. Salmonella Typhimurium ST19 strains are strong biofilm producers and can survive desiccation compared to Salmonella Typhimurium ST313 that form weak biofilms and survive poorly following desiccation. Our data suggest that like Salmonella Typhi, Salmonella Typhimurium ST313 lack mechanisms that allow it to persist in the environment. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  12. Effect of Food Residues in Biofilm Formation on Stainless Steel and Polystyrene Surfaces by Salmonella enterica Strains Isolated from Poultry Houses

    OpenAIRE

    Paz-Méndez, Alba María; Lamas, Alexandre; Vázquez, Beatriz; Miranda, José Manuel; Cepeda, Alberto; Franco, Carlos Manuel

    2017-01-01

    Salmonella spp. is a major food-borne pathogen around the world. The ability of Salmonella to produce biofilm is one of the main obstacles in reducing the prevalence of these bacteria in the food chain. Most of Salmonella biofilm studies found in the literature used laboratory growth media. However, in the food chain, food residues are the principal source of nutrients of Salmonella. In this study, the biofilm formation, morphotype, and motility of 13 Salmonella strains belonging to three dif...

  13. Effects of propolis from Brazil and Bulgaria on Salmonella serovars

    Directory of Open Access Journals (Sweden)

    R. O. Orsi

    2007-01-01

    Full Text Available Propolis shows biological properties such as antibacterial action. This bee product has a complex chemical composition, which depends on the local flora where it is produced. Salmonella serovars are responsible for human diseases that range from localized gastroenteritis to systemic infections. The aim of the present study was to investigate the susceptibility of Salmonella strains, isolated from food and infectious processes, to the antibacterial action of Brazilian and Bulgarian propolis, as well as to determine the behavior of these bacteria, according to the incubation period, in medium plus propolis. Dilution of ethanolic extract of propolis in agar was the used method. Brazilian and Bulgarian propolis showed an antibacterial action against all Salmonella serovars. The minimal inhibitory concentrations (MIC of propolis were similar, although they were collected in different geographic regions. Salmonella typhimurium, isolated from human infection, was more resistant to propolis than Salmonella enteritidis.

  14. Breve historia de la literatura escéptica.

    OpenAIRE

    Castany Prado, Bernat

    2009-01-01

    Proponemos el término"literatura escéptica" para referirnos a aquellas obras cuyo tema y convicción fundamental es la incapacidad cognoscitiva del ser humano y sus implicaciones éticas, políticas, religiosas y existenciales. Ciertamente, a cada doctrina o sensibilidad filosófica corresponde, de forma compleja y bilateral, una constelación de rasgos literarios, más o menos constantes, en los diversos ámbitos del estilo, las estructuras narrativas, los temas o los símbolos.

  15. Exploiting host immunity: the Salmonella paradigm

    Science.gov (United States)

    Behnsen, Judith; Perez-Lopez, Araceli; Nuccio, Sean-Paul; Raffatellu, Manuela

    2014-01-01

    Pathogens have evolved clever strategies to evade and in some cases exploit the attacks of an activated immune system. Salmonella enterica is one such pathogen, exploiting multiple aspects of host defense to promote its replication in the host. Here we review recent findings on the mechanisms by which Salmonella establishes systemic and chronic infection, including strategies involving manipulation of innate immune signaling and inflammatory forms of cell death, as well as immune evasion by establishing residency in M2 macrophages. We also examine recent evidence showing that the oxidative environment and the high levels of antimicrobial proteins produced in response to localized Salmonella gastrointestinal infection enable the pathogen to successfully outcompete the resident gut microbiota. PMID:25582038

  16. Protein interactions and regulation of EscA in enterohemorrhagic E. coli.

    Directory of Open Access Journals (Sweden)

    Ching-Nan Lin

    Full Text Available Infections caused by enterohemorrhagic Escherichia coli (EHEC can lead to diarrhea with abdominal cramps and sometimes are complicated by severe hemolytic uremic syndrome. EHEC secretes effector proteins into host cells through a type III secretion system that is composed of proteins encoded by a chromosomal island, locus for the enterocyte effacement (LEE. EspA is the major component of the filamentous structure connecting the bacteria and the host's cells. Synthesis and secretion of EspA must be carefully controlled since the protein is prone to polymerize. CesAB, CesA2, and EscL have been identified as being able to interact with EspA. Furthermore, the intracellular level of EspA declines when cesAB, cesA2, and escL are individually deleted. Here, we report a LEE gene named l0033, which also affects the intracellular level of EspA. We renamed l0033 as escA since its counterpart in enteropathogenic E. coli has been recently described. Similar to CesAB, EscL, and CesA2, EscA interacts with EspA and enhances the protein stability of EspA. However, EscA is also able to interact with inner membrane-associated EscL, CesA2, and EscN, but not with cytoplasmic CesAB. In terms of gene organizations, escA locates in LEE3. Expression of EscA is faithfully regulated via Mpc, the first gene product of LEE3. Since Mpc is tightly regulated to low level, we suggest that EscA is highly synchronized and critical to the process of escorting EspA to its final destination.

  17. Salmonella enteritidis ventriculitis

    National Research Council Canada - National Science Library

    Johan, A J; Hung, L C; Norlijah, O

    2013-01-01

    .... We present a case of Salmonella enteritidis meningitis in a six week old female who presented with a one week history of fever, diarrhea and seizures which was unsuccessfully treated with a third...

  18. Cell lines and Salmonella

    NARCIS (Netherlands)

    de Jonge R; Hendriks H; Garssen J; MGB; LPI

    2001-01-01

    Infectie met Salmonella kan gepaard gaan met de invasie van darmepitheelcellen. De aan de invasie voorafgaande aanhechting leidt reeds tot de transmigratie van witte bloedcellen (neutrofielen) vanuit de bloedbaan naar het epitheelweefsel. De migratie wordt gestimuleerd door de productie van

  19. Resistance to antimicrobials drugs and control measures of Salmonella spp in the poultry industry

    Directory of Open Access Journals (Sweden)

    Velhner Maja

    2013-01-01

    Full Text Available The worldwide prevalence of multiple resistant Salmonella spp is described. Clonally distributed Salmonella Enteritidis PT4 and Salmonella Typhimurium DT104 are among the most pathogenic strains for humans. Recently there have been reports on the prevalence of ST “like” monophasic 4(5,12:i strains in some countries. Vaccination strategy and antimicorbial agent therapy is also briefly discussed. Products of animal origin must be safe and without the risk of antimicrobial resistance. Subsequently, the good management practice at farm level and HACCP in feed factories are required to cope with salmonella infections. Poultry producers in developed countries have been motivated to participate in salmonella control programs, because of public awareness on safe food and risks in the food chain. Export of poultry and poultry products is more successful in the regions where Salmonella Enteritidis and Salmonella Typhimurium have been eradicated. [Projekat Ministarstva nauke Republike Srbije, br. TR31071

  20. Reducing airborne pathogens, dust and Salmonella transmission in experimental hatching cabinets using an electrostatic space charge system.

    Science.gov (United States)

    Mitchell, B W; Buhr, R J; Berrang, M E; Bailey, J S; Cox, N A

    2002-01-01

    Electrostatic charging of particles in enclosed spaces has been shown to be an effective means of reducing airborne dust. Dust generated during the hatching process has been strongly implicated in Salmonella transmission, which complicates the cleaning and disinfecting processes for hatchers. Following two preliminary trials in which dust reduction was measured, four trials were conducted to evaluate the effectiveness of an electrostatic space charge system (ESCS) on the levels of total aerobic bacteria (TPC), enterobacteriaceae (ENT), and Salmonella within an experimental hatching cabinet. The ESCS was placed in a hatching cabinet that was approximately 50% full of 18-d-old broiler hatching eggs. The ESCS operated continuously to generate a strong negative electrostatic charge throughout the cabinet through hatching, and dust was collected in grounded trays containing water and a degreaser. An adjacent hatching cabinet served as an untreated control. Air samples from hatchers were collected daily, and sample chicks from each hatcher were grown out to 7 d of age for cecal analysis in three of the trials. The ESCS significantly (P < 0.05) reduced TPC and ENT by 85 to 93%. Dust concentration was significantly reduced (P < 0.0001) during the preliminary trials with an average reduction of 93.6%. The number of Salmonella per gram of cecal contents in birds grown to 7 d of age was significantly (P < 0.001) reduced by an average log10 3.4 cfu/g. This ionization technology is relatively inexpensive and could be used to reduce airborne bacteria and dust within the hatching cabinet.

  1. Salmonella and Campylobacter: Antimicrobial resistance and bacteriophage control in poultry.

    Science.gov (United States)

    Grant, Ar'Quette; Hashem, Fawzy; Parveen, Salina

    2016-02-01

    Salmonella and Campylobacter are major causes of foodborne related illness and are traditionally associated with consuming undercooked poultry and/or consuming products that have been cross contaminated with raw poultry. Many of the isolated Salmonella and Campylobacter that can cause disease have displayed antimicrobial resistance phenotypes. Although poultry producers have reduced on-the-farm overuse of antimicrobials, antimicrobial resistant Salmonella and Campylobacter strains still persist. One method of bio-control, that is producing promising results, is the use of lytic bacteriophages. This review will highlight the current emergence and persistence of antimicrobial resistant Salmonella and Campylobacter recovered from poultry as well as bacteriophage research interventions and limitations. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. An assessment of soybeans and other vegetable proteins as source of salmonella contamination in pig production

    Directory of Open Access Journals (Sweden)

    Häggblom Per

    2010-02-01

    Full Text Available Abstract Background The impact of salmonella contaminated feed ingredients on the risk for spreading salmonella to pigs was assessed in response to two incidences when salmonella was spread by feed from two feed mills to 78 swine producing herds. Methods The assessment was based on results from the salmonella surveillance of feed ingredients before introduction to feed mills and from HACCP - based surveillance of the feed mills. Results from the mills of the Company (A that produced the salmonella contaminated feed, were by the Chi. Square test compared to the results from all the other (B - E feed producers registered in Sweden. Isolated serovars were compared to serovars from human cases of salmonellosis. Results Salmonella (28 serovars was frequently isolated from imported consignments of soybean meal (14.6% and rape seed meal (10.0%. Company A largely imported soybean meal from crushing plants with a history of unknown or frequent salmonella contamination. The risk for consignments of vegetable proteins to be salmonella contaminated was 2.4 times (P Conclusions Salmonella contaminated feed ingredients are an important source for introducing salmonella into the feed and food chain. Effective HACCP-based control and associated corrective actions are required to prevent salmonella contamination of feed. Efforts should be taken to prevent salmonella contamination already at the crushing plants. This is challenge for the EU - feed industry due to the fact that 98% of the use of soybean/meal, an essential feed ingredient, is imported from crushing plants of third countries usually with an unknown salmonella status.

  3. Salmonella in sesame seed products.

    Science.gov (United States)

    Brockmann, Stefan O; Piechotowski, Isolde; Kimmig, Peter

    2004-01-01

    In the context of an international outbreak of multiresistant Salmonella Typhimurium DT 104 that was correlated to the consumption of halvah ("helva," an Asian candy made from sesame seed), we examined several sesame seed products for the occurrence of Salmonella. Of 117 ready-to-eat food items containing sesame, we isolated salmonellae from 11 (9.4%) samples. In addition to finding Salmonella Typhimurium DT 104 in the halvah involved in the outbreak, we also isolated different Salmonella Typhimurium strains out of halvah from other manufacturers and countries of origin, as well as Salmonella Offa, Salmonella Tennessee, and Salmonella Poona from sesame paste (tahini) and sesame seed, which is sold for raw consumption in cereals.

  4. European Society of Cardiology (ESC) Congress Report from London 2015.

    Science.gov (United States)

    Nishiguchi, Tsuyoshi; Akasaka, Takashi

    2015-01-01

    The Annual Congress of the European Society of Cardiology (ESC) was held in London from 29 August to 2 September 2015. It is the leading conference in cardiology in the world, with presentations on the latest scientific discoveries, innovations, technology, education, and clinical practices. More than 32,000 delegates and 5,000 exhibitors from 140 countries participated, sharing a number of scientific presentations, including 28 clinical hot lines, 18 clinical trial updates, 20 registry studies, 12 basic and translational science hot line studies, and 4,533 abstract studies. Japan had the highest number of accepted abstracts at the Congress, indicating the great contribution of Japanese scientists and the Japanese Circulation Society.

  5. File list: His.PSC.50.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.50.AllAg.mESC_derived_cardiac_cells mm9 Histone Pluripotent stem cell mESC derived cardiac...917,SRX305916,SRX305901,SRX305899,SRX305900,SRX305898,SRX305897 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.50.AllAg.mESC_derived_cardiac_cells.bed ...

  6. File list: Unc.PSC.50.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.50.AllAg.mESC_derived_cardiac_cells mm9 Unclassified Pluripotent stem cell mESC derived cardiac... cells SRX685645,SRX685643,SRX685642,SRX685644 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.50.AllAg.mESC_derived_cardiac_cells.bed ...

  7. File list: Unc.PSC.05.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.mESC_derived_cardiac_cells mm9 Unclassified Pluripotent stem cell mESC derived cardiac... cells SRX685643,SRX685645,SRX685642,SRX685644 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.05.AllAg.mESC_derived_cardiac_cells.bed ...

  8. File list: His.PSC.05.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.mESC_derived_cardiac_cells mm9 Histone Pluripotent stem cell mESC derived cardiac...907,SRX305897,SRX305899,SRX305901,SRX305927,SRX305915,SRX305928 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.05.AllAg.mESC_derived_cardiac_cells.bed ...

  9. File list: Oth.PSC.20.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.AllAg.mESC_derived_cardiac_cells mm9 TFs and others Pluripotent stem cell mESC derived cardiac...30,SRX1437359 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.AllAg.mESC_derived_cardiac_cells.bed ...

  10. File list: Oth.PSC.50.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.50.AllAg.mESC_derived_cardiac_cells mm9 TFs and others Pluripotent stem cell mESC derived cardiac...359,SRX994830 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.50.AllAg.mESC_derived_cardiac_cells.bed ...

  11. File list: NoD.PSC.50.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.mESC_derived_cardiac_cells mm9 No description Pluripotent stem cell mESC derived cardiac... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.50.AllAg.mESC_derived_cardiac_cells.bed ...

  12. File list: His.PSC.20.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.mESC_derived_cardiac_cells mm9 Histone Pluripotent stem cell mESC derived cardiac...915,SRX305901,SRX305916,SRX305898,SRX305917,SRX305900,SRX305897 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.20.AllAg.mESC_derived_cardiac_cells.bed ...

  13. File list: InP.PSC.50.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.50.AllAg.mESC_derived_cardiac_cells mm9 Input control Pluripotent stem cell mESC derived cardiac...sciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.50.AllAg.mESC_derived_cardiac_cells.bed ...

  14. File list: DNS.PSC.10.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.mESC_derived_cardiac_cells mm9 DNase-seq Pluripotent stem cell mESC derived cardiac... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.10.AllAg.mESC_derived_cardiac_cells.bed ...

  15. File list: ALL.PSC.10.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.mESC_derived_cardiac_cells mm9 All antigens Pluripotent stem cell mESC derived cardiac...7359 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.10.AllAg.mESC_derived_cardiac_cells.bed ...

  16. File list: ALL.PSC.20.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.20.AllAg.mESC_derived_cardiac_cells mm9 All antigens Pluripotent stem cell mESC derived cardiac...5897 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.20.AllAg.mESC_derived_cardiac_cells.bed ...

  17. File list: ALL.PSC.05.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.mESC_derived_cardiac_cells mm9 All antigens Pluripotent stem cell mESC derived cardiac...7360 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.05.AllAg.mESC_derived_cardiac_cells.bed ...

  18. File list: ALL.PSC.50.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.50.AllAg.mESC_derived_cardiac_cells mm9 All antigens Pluripotent stem cell mESC derived cardiac...5897 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.50.AllAg.mESC_derived_cardiac_cells.bed ...

  19. File list: DNS.PSC.20.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.mESC_derived_cardiac_cells mm9 DNase-seq Pluripotent stem cell mESC derived cardiac... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.20.AllAg.mESC_derived_cardiac_cells.bed ...

  20. File list: Unc.PSC.10.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.10.AllAg.mESC_derived_cardiac_cells mm9 Unclassified Pluripotent stem cell mESC derived cardiac... cells SRX685643,SRX685645,SRX685642,SRX685644 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.10.AllAg.mESC_derived_cardiac_cells.bed ...

  1. File list: Oth.PSC.10.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.AllAg.mESC_derived_cardiac_cells mm9 TFs and others Pluripotent stem cell mESC derived cardiac...30,SRX1437359 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.10.AllAg.mESC_derived_cardiac_cells.bed ...

  2. File list: InP.PSC.20.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.20.AllAg.mESC_derived_cardiac_cells mm9 Input control Pluripotent stem cell mESC derived cardiac...sciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.20.AllAg.mESC_derived_cardiac_cells.bed ...

  3. File list: Unc.PSC.20.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.20.AllAg.mESC_derived_cardiac_cells mm9 Unclassified Pluripotent stem cell mESC derived cardiac... cells SRX685645,SRX685643,SRX685642,SRX685644 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.20.AllAg.mESC_derived_cardiac_cells.bed ...

  4. File list: Pol.PSC.05.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.mESC_derived_cardiac_cells mm9 RNA polymerase Pluripotent stem cell mESC derived cardiac... cells SRX305934,SRX305933,SRX305932,SRX305935 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.05.AllAg.mESC_derived_cardiac_cells.bed ...

  5. File list: DNS.PSC.05.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.mESC_derived_cardiac_cells mm9 DNase-seq Pluripotent stem cell mESC derived cardiac... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.05.AllAg.mESC_derived_cardiac_cells.bed ...

  6. File list: NoD.PSC.20.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.20.AllAg.mESC_derived_cardiac_cells mm9 No description Pluripotent stem cell mESC derived cardiac... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.20.AllAg.mESC_derived_cardiac_cells.bed ...

  7. File list: InP.PSC.10.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.10.AllAg.mESC_derived_cardiac_cells mm9 Input control Pluripotent stem cell mESC derived cardiac...sciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.10.AllAg.mESC_derived_cardiac_cells.bed ...

  8. File list: His.PSC.10.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.mESC_derived_cardiac_cells mm9 Histone Pluripotent stem cell mESC derived cardiac...928,SRX305927,SRX305926,SRX305915,SRX305900,SRX305916,SRX305917 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.10.AllAg.mESC_derived_cardiac_cells.bed ...

  9. File list: NoD.PSC.05.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.mESC_derived_cardiac_cells mm9 No description Pluripotent stem cell mESC derived cardiac... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.05.AllAg.mESC_derived_cardiac_cells.bed ...

  10. File list: Pol.PSC.50.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.mESC_derived_cardiac_cells mm9 RNA polymerase Pluripotent stem cell mESC derived cardiac... cells SRX305933,SRX305932,SRX305935,SRX305934 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.50.AllAg.mESC_derived_cardiac_cells.bed ...

  11. File list: DNS.PSC.50.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.50.AllAg.mESC_derived_cardiac_cells mm9 DNase-seq Pluripotent stem cell mESC derived cardiac... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.50.AllAg.mESC_derived_cardiac_cells.bed ...

  12. File list: InP.PSC.05.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.05.AllAg.mESC_derived_cardiac_cells mm9 Input control Pluripotent stem cell mESC derived cardiac...sciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.05.AllAg.mESC_derived_cardiac_cells.bed ...

  13. File list: NoD.PSC.10.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.10.AllAg.mESC_derived_cardiac_cells mm9 No description Pluripotent stem cell mESC derived cardiac... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.10.AllAg.mESC_derived_cardiac_cells.bed ...

  14. File list: Pol.PSC.20.AllAg.mESC_derived_cardiac_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.mESC_derived_cardiac_cells mm9 RNA polymerase Pluripotent stem cell mESC derived cardiac... cells SRX305933,SRX305932,SRX305935,SRX305934 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.20.AllAg.mESC_derived_cardiac_cells.bed ...

  15. File list: InP.PSC.05.AllAg.hESC_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.05.AllAg.hESC_derived_fibroblasts hg19 Input control Pluripotent stem cell hESC derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.05.AllAg.hESC_derived_fibroblasts.bed ...

  16. File list: His.PSC.10.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.mESC_derived_neural_cells mm9 Histone Pluripotent stem cell mESC derived...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.10.AllAg.mESC_derived_neural_cells.bed ...

  17. File list: His.PSC.05.AllAg.hESC_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.hESC_derived_fibroblasts hg19 Histone Pluripotent stem cell hESC derived...archive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.05.AllAg.hESC_derived_fibroblasts.bed ...

  18. File list: Unc.PSC.05.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.hESC_derived_trophoblast_cells hg19 Unclassified Pluripotent stem cell hESC derived... trophoblast cells SRX378285 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.05.AllAg.hESC_derived_trophoblast_cells.bed ...

  19. File list: Pol.PSC.20.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.hESC_derived_trophoblast_cells hg19 RNA polymerase Pluripotent stem cell hESC derived... trophoblast cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.20.AllAg.hESC_derived_trophoblast_cells.bed ...

  20. File list: Pol.PSC.20.AllAg.hESC_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.hESC_derived_fibroblasts hg19 RNA polymerase Pluripotent stem cell hESC derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.20.AllAg.hESC_derived_fibroblasts.bed ...

  1. File list: NoD.PSC.50.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.hESC_derived_neural_crests hg19 No description Pluripotent stem cell hESC derived... neural crests http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.PSC.50.AllAg.hESC_derived_neural_crests.bed ...

  2. File list: DNS.PSC.05.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.hESC_derived_neural_crests hg19 DNase-seq Pluripotent stem cell hESC derived... neural crests http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.05.AllAg.hESC_derived_neural_crests.bed ...

  3. File list: Unc.PSC.05.AllAg.hESC_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.hESC_derived_fibroblasts hg19 Unclassified Pluripotent stem cell hESC derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.05.AllAg.hESC_derived_fibroblasts.bed ...

  4. File list: InP.PSC.10.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.10.AllAg.mESC_derived_neural_cells mm9 Input control Pluripotent stem cell mESC derived...77,SRX1214070,SRX518051,SRX604258,SRX810565,SRX810564,SRX604259 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.10.AllAg.mESC_derived_neural_cells.bed ...

  5. File list: ALL.PSC.05.AllAg.hESC_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.hESC_derived_fibroblasts hg19 All antigens Pluripotent stem cell hESC derived...://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.05.AllAg.hESC_derived_fibroblasts.bed ...

  6. File list: DNS.PSC.05.AllAg.hESC_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.hESC_derived_fibroblasts hg19 DNase-seq Pluripotent stem cell hESC derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.05.AllAg.hESC_derived_fibroblasts.bed ...

  7. File list: His.PSC.20.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.mESC_derived_neural_cells mm9 Histone Pluripotent stem cell mESC derived...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.20.AllAg.mESC_derived_neural_cells.bed ...

  8. File list: NoD.PSC.05.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.hESC_derived_neural_cells hg19 No description Pluripotent stem cell hESC derived...edbc.jp/kyushu-u/hg19/assembled/NoD.PSC.05.AllAg.hESC_derived_neural_cells.bed ...

  9. File list: InP.PSC.10.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.10.AllAg.hESC_derived_trophoblast_cells hg19 Input control Pluripotent stem cell hESC derived... trophoblast cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.10.AllAg.hESC_derived_trophoblast_cells.bed ...

  10. File list: DNS.PSC.20.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.hESC_derived_trophoblast_cells hg19 DNase-seq Pluripotent stem cell hESC derived... trophoblast cells SRX121240,SRX134724,SRX121254 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.20.AllAg.hESC_derived_trophoblast_cells.bed ...

  11. File list: Pol.PSC.50.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.hESC_derived_neural_crests hg19 RNA polymerase Pluripotent stem cell hESC derived... neural crests http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.50.AllAg.hESC_derived_neural_crests.bed ...

  12. File list: Pol.PSC.50.AllAg.hESC_derived_ectodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.hESC_derived_ectodermal_cells hg19 RNA polymerase Pluripotent stem cell hESC derived... ectodermal cells SRX702062 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.50.AllAg.hESC_derived_ectodermal_cells.bed ...

  13. File list: Pol.PSC.10.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.mESC_derived_neural_cells mm9 RNA polymerase Pluripotent stem cell mESC derived...RX213760,SRX213764 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.10.AllAg.mESC_derived_neural_cells.bed ...

  14. File list: Pol.PSC.10.AllAg.hESC_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.hESC_derived_fibroblasts hg19 RNA polymerase Pluripotent stem cell hESC derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.10.AllAg.hESC_derived_fibroblasts.bed ...

  15. File list: ALL.PSC.10.AllAg.hESC_derived_ectodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.hESC_derived_ectodermal_cells hg19 All antigens Pluripotent stem cell hESC derived...p/kyushu-u/hg19/assembled/ALL.PSC.10.AllAg.hESC_derived_ectodermal_cells.bed ...

  16. File list: NoD.PSC.50.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.hESC_derived_trophoblast_cells hg19 No description Pluripotent stem cell hESC derived...5230,SRX135232,SRX081157,SRX081154 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.PSC.50.AllAg.hESC_derived_trophoblast_cells.bed ...

  17. File list: Oth.PSC.20.AllAg.hESC_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.AllAg.hESC_derived_fibroblasts hg19 TFs and others Pluripotent stem cell hESC derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.PSC.20.AllAg.hESC_derived_fibroblasts.bed ...

  18. File list: NoD.PSC.50.AllAg.hESC_derived_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.hESC_derived_fibroblasts hg19 No description Pluripotent stem cell hESC derived... fibroblasts http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.PSC.50.AllAg.hESC_derived_fibroblasts.bed ...

  19. File list: InP.PSC.20.AllAg.hESC_derived_ectodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.20.AllAg.hESC_derived_ectodermal_cells hg19 Input control Pluripotent stem cell hESC derived... ectodermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.20.AllAg.hESC_derived_ectodermal_cells.bed ...

  20. File list: ALL.PSC.05.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.hESC_derived_trophoblast_cells hg19 All antigens Pluripotent stem cell hESC derived...barchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.05.AllAg.hESC_derived_trophoblast_cells.bed ...

  1. File list: InP.PSC.05.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.05.AllAg.hESC_derived_trophoblast_cells hg19 Input control Pluripotent stem cell hESC derived... trophoblast cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.05.AllAg.hESC_derived_trophoblast_cells.bed ...

  2. File list: Unc.PSC.10.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.10.AllAg.mESC_derived_neural_cells mm9 Unclassified Pluripotent stem cell mESC derived...,SRX213757,SRX213761 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.10.AllAg.mESC_derived_neural_cells.bed ...

  3. File list: Pol.PSC.10.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.hESC_derived_trophoblast_cells hg19 RNA polymerase Pluripotent stem cell hESC derived... trophoblast cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.10.AllAg.hESC_derived_trophoblast_cells.bed ...

  4. File list: Pol.PSC.05.AllAg.hESC_derived_ectodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.hESC_derived_ectodermal_cells hg19 RNA polymerase Pluripotent stem cell hESC derived... ectodermal cells SRX702062 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.05.AllAg.hESC_derived_ectodermal_cells.bed ...

  5. File list: DNS.PSC.10.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.hESC_derived_trophoblast_cells hg19 DNase-seq Pluripotent stem cell hESC derived... trophoblast cells SRX121240,SRX134724,SRX121254 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.10.AllAg.hESC_derived_trophoblast_cells.bed ...

  6. File list: InP.PSC.50.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.50.AllAg.hESC_derived_trophoblast_cells hg19 Input control Pluripotent stem cell hESC derived... trophoblast cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.50.AllAg.hESC_derived_trophoblast_cells.bed ...

  7. File list: InP.PSC.20.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.20.AllAg.hESC_derived_trophoblast_cells hg19 Input control Pluripotent stem cell hESC derived... trophoblast cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.20.AllAg.hESC_derived_trophoblast_cells.bed ...

  8. File list: NoD.PSC.20.AllAg.hESC_derived_trophoblast_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.20.AllAg.hESC_derived_trophoblast_cells hg19 No description Pluripotent stem cell hESC derived...1157,SRX135234,SRX101257,SRX081154 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.PSC.20.AllAg.hESC_derived_trophoblast_cells.bed ...

  9. File list: DNS.PSC.10.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.mESC_derived_neural_cells mm9 DNase-seq Pluripotent stem cell mESC derived... neural cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.10.AllAg.mESC_derived_neural_cells.bed ...

  10. File list: His.PSC.10.AllAg.hESC_derived_ectodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.hESC_derived_ectodermal_cells hg19 Histone Pluripotent stem cell hESC derived... ectodermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.10.AllAg.hESC_derived_ectodermal_cells.bed ...

  11. File list: ALL.PSC.10.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.mESC_derived_pancreatic_cells mm9 All antigens Pluripotent stem cell mESC derived pancreati...cedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.10.AllAg.mESC_derived_pancreatic_cells.bed ...

  12. File list: NoD.PSC.20.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.20.AllAg.mESC_derived_pancreatic_cells mm9 No description Pluripotent stem cell mESC derived pancrea...tic cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.20.AllAg.mESC_derived_pancreatic_cells.bed ...

  13. File list: Unc.PSC.10.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.10.AllAg.mESC_derived_pancreatic_cells mm9 Unclassified Pluripotent stem cell mESC derived pancreati...c cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.10.AllAg.mESC_derived_pancreatic_cells.bed ...

  14. File list: His.PSC.20.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.mESC_derived_pancreatic_cells mm9 Histone Pluripotent stem cell mESC derived pancreatic... cells SRX146012,SRX146011 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.20.AllAg.mESC_derived_pancreatic_cells.bed ...

  15. File list: InP.PSC.05.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.05.AllAg.mESC_derived_pancreatic_cells mm9 Input control Pluripotent stem cell mESC derived pancreat...ic cells SRX146010,SRX146009 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.05.AllAg.mESC_derived_pancreatic_cells.bed ...

  16. File list: DNS.PSC.50.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.50.AllAg.mESC_derived_pancreatic_cells mm9 DNase-seq Pluripotent stem cell mESC derived pancreatic... cells SRX404487 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.50.AllAg.mESC_derived_pancreatic_cells.bed ...

  17. File list: NoD.PSC.50.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.mESC_derived_pancreatic_cells mm9 No description Pluripotent stem cell mESC derived pancrea...tic cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.50.AllAg.mESC_derived_pancreatic_cells.bed ...

  18. File list: Pol.PSC.10.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.mESC_derived_pancreatic_cells mm9 RNA polymerase Pluripotent stem cell mESC derived pancrea...tic cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.10.AllAg.mESC_derived_pancreatic_cells.bed ...

  19. File list: Oth.PSC.10.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.AllAg.mESC_derived_pancreatic_cells mm9 TFs and others Pluripotent stem cell mESC derived pancrea...tic cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.10.AllAg.mESC_derived_pancreatic_cells.bed ...

  20. File list: ALL.PSC.50.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.50.AllAg.mESC_derived_pancreatic_cells mm9 All antigens Pluripotent stem cell mESC derived pancreati...cedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.50.AllAg.mESC_derived_pancreatic_cells.bed ...

  1. File list: DNS.PSC.20.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.mESC_derived_pancreatic_cells mm9 DNase-seq Pluripotent stem cell mESC derived pancreatic... cells SRX404487 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.20.AllAg.mESC_derived_pancreatic_cells.bed ...

  2. File list: Unc.PSC.50.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.50.AllAg.mESC_derived_pancreatic_cells mm9 Unclassified Pluripotent stem cell mESC derived pancreati...c cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.50.AllAg.mESC_derived_pancreatic_cells.bed ...

  3. File list: ALL.PSC.05.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.mESC_derived_pancreatic_cells mm9 All antigens Pluripotent stem cell mESC derived pancreati...cedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.05.AllAg.mESC_derived_pancreatic_cells.bed ...

  4. File list: His.PSC.50.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.50.AllAg.mESC_derived_pancreatic_cells mm9 Histone Pluripotent stem cell mESC derived pancreatic... cells SRX146012,SRX146011 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.50.AllAg.mESC_derived_pancreatic_cells.bed ...

  5. File list: NoD.PSC.05.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.mESC_derived_pancreatic_cells mm9 No description Pluripotent stem cell mESC derived pancrea...tic cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.05.AllAg.mESC_derived_pancreatic_cells.bed ...

  6. File list: Pol.PSC.50.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.mESC_derived_pancreatic_cells mm9 RNA polymerase Pluripotent stem cell mESC derived pancrea...tic cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.50.AllAg.mESC_derived_pancreatic_cells.bed ...

  7. File list: Unc.PSC.05.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.mESC_derived_pancreatic_cells mm9 Unclassified Pluripotent stem cell mESC derived pancreati...c cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.05.AllAg.mESC_derived_pancreatic_cells.bed ...

  8. File list: DNS.PSC.05.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.mESC_derived_pancreatic_cells mm9 DNase-seq Pluripotent stem cell mESC derived pancreatic... cells SRX404487 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.05.AllAg.mESC_derived_pancreatic_cells.bed ...

  9. File list: InP.PSC.20.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.20.AllAg.mESC_derived_pancreatic_cells mm9 Input control Pluripotent stem cell mESC derived pancreat...ic cells SRX146010,SRX146009 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.20.AllAg.mESC_derived_pancreatic_cells.bed ...

  10. File list: ALL.PSC.20.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.20.AllAg.mESC_derived_pancreatic_cells mm9 All antigens Pluripotent stem cell mESC derived pancreati...cedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.20.AllAg.mESC_derived_pancreatic_cells.bed ...

  11. File list: His.PSC.05.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.mESC_derived_pancreatic_cells mm9 Histone Pluripotent stem cell mESC derived pancreatic... cells SRX146012,SRX146011 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.05.AllAg.mESC_derived_pancreatic_cells.bed ...

  12. File list: NoD.PSC.10.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.10.AllAg.mESC_derived_pancreatic_cells mm9 No description Pluripotent stem cell mESC derived pancrea...tic cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.10.AllAg.mESC_derived_pancreatic_cells.bed ...

  13. File list: His.PSC.10.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.mESC_derived_pancreatic_cells mm9 Histone Pluripotent stem cell mESC derived pancreatic... cells SRX146012,SRX146011 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.10.AllAg.mESC_derived_pancreatic_cells.bed ...

  14. File list: InP.PSC.10.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.10.AllAg.mESC_derived_pancreatic_cells mm9 Input control Pluripotent stem cell mESC derived pancreat...ic cells SRX146010,SRX146009 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.10.AllAg.mESC_derived_pancreatic_cells.bed ...

  15. File list: Oth.PSC.05.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.05.AllAg.mESC_derived_pancreatic_cells mm9 TFs and others Pluripotent stem cell mESC derived pancrea...tic cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.05.AllAg.mESC_derived_pancreatic_cells.bed ...

  16. File list: Oth.PSC.50.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.50.AllAg.mESC_derived_pancreatic_cells mm9 TFs and others Pluripotent stem cell mESC derived pancrea...tic cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.50.AllAg.mESC_derived_pancreatic_cells.bed ...

  17. File list: Oth.PSC.20.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.AllAg.mESC_derived_pancreatic_cells mm9 TFs and others Pluripotent stem cell mESC derived pancrea...tic cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.AllAg.mESC_derived_pancreatic_cells.bed ...

  18. File list: DNS.PSC.10.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.mESC_derived_pancreatic_cells mm9 DNase-seq Pluripotent stem cell mESC derived pancreatic... cells SRX404487 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.10.AllAg.mESC_derived_pancreatic_cells.bed ...

  19. File list: Pol.PSC.05.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.mESC_derived_pancreatic_cells mm9 RNA polymerase Pluripotent stem cell mESC derived pancrea...tic cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.05.AllAg.mESC_derived_pancreatic_cells.bed ...

  20. File list: InP.PSC.50.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.50.AllAg.mESC_derived_pancreatic_cells mm9 Input control Pluripotent stem cell mESC derived pancreat...ic cells SRX146010,SRX146009 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.50.AllAg.mESC_derived_pancreatic_cells.bed ...

  1. File list: Unc.PSC.20.AllAg.mESC_derived_pancreatic_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.20.AllAg.mESC_derived_pancreatic_cells mm9 Unclassified Pluripotent stem cell mESC derived pancreati...c cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.20.AllAg.mESC_derived_pancreatic_cells.bed ...

  2. File list: NoD.PSC.10.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.10.AllAg.mESCs,_differentiated mm9 No description Pluripotent stem cell mESCs, different...iated http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.10.AllAg.mESCs,_differentiated.bed ...

  3. File list: InP.PSC.50.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.50.AllAg.mESCs,_differentiated mm9 Input control Pluripotent stem cell mESCs, different...,SRX388447 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.50.AllAg.mESCs,_differentiated.bed ...

  4. File list: NoD.PSC.20.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.20.AllAg.mESCs,_differentiated mm9 No description Pluripotent stem cell mESCs, different...iated http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.20.AllAg.mESCs,_differentiated.bed ...

  5. File list: InP.PSC.10.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.10.AllAg.mESCs,_differentiated mm9 Input control Pluripotent stem cell mESCs, different...,SRX388447 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.10.AllAg.mESCs,_differentiated.bed ...

  6. File list: InP.PSC.20.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.20.AllAg.mESCs,_differentiated mm9 Input control Pluripotent stem cell mESCs, different...,SRX388447 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.20.AllAg.mESCs,_differentiated.bed ...

  7. File list: NoD.PSC.05.AllAg.mESCs,_differentiated [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.mESCs,_differentiated mm9 No description Pluripotent stem cell mESCs, different...iated http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.05.AllAg.mESCs,_differentiated.bed ...

  8. File list: Oth.PSC.05.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.05.AllAg.hESC_derived_neural_crests hg19 TFs and others Pluripotent stem cell hESC derived neural...X1091546,SRX1091550,SRX059360,SRX059368,SRX059367 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.PSC.05.AllAg.hESC_derived_neural_crests.bed ...

  9. File list: Oth.PSC.10.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.AllAg.hESC_derived_neural_crests hg19 TFs and others Pluripotent stem cell hESC derived neural...X1091546,SRX1091550,SRX059360,SRX059368,SRX059367 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.PSC.10.AllAg.hESC_derived_neural_crests.bed ...

  10. File list: Pol.PSC.10.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.hESC_derived_neural_crests hg19 RNA polymerase Pluripotent stem cell hESC derived neural... crests http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.10.AllAg.hESC_derived_neural_crests.bed ...

  11. File list: InP.PSC.10.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.10.AllAg.hESC_derived_neural_crests hg19 Input control Pluripotent stem cell hESC derived neural... crests SRX1091573,SRX059369,SRX059361 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.10.AllAg.hESC_derived_neural_crests.bed ...

  12. File list: Pol.PSC.05.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.hESC_derived_neural_cells hg19 RNA polymerase Pluripotent stem cell hESC derived neural... cells SRX190259 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.05.AllAg.hESC_derived_neural_cells.bed ...

  13. File list: His.PSC.05.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.mESC_derived_neural_cells mm9 Histone Pluripotent stem cell mESC derived neural...tp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.05.AllAg.mESC_derived_neural_cells.bed ...

  14. File list: NoD.PSC.20.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.20.AllAg.hESC_derived_neural_crests hg19 No description Pluripotent stem cell hESC derived neural... crests http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.PSC.20.AllAg.hESC_derived_neural_crests.bed ...

  15. File list: Pol.PSC.20.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.mESC_derived_neural_cells mm9 RNA polymerase Pluripotent stem cell mESC derived neural...RX213760,SRX213764 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.20.AllAg.mESC_derived_neural_cells.bed ...

  16. File list: Oth.PSC.50.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.50.AllAg.mESC_derived_neural_cells mm9 TFs and others Pluripotent stem cell mESC derived neural...13762,SRX213759,SRX352995 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.50.AllAg.mESC_derived_neural_cells.bed ...

  17. File list: DNS.PSC.10.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.hESC_derived_neural_cells hg19 DNase-seq Pluripotent stem cell hESC derived neural... cells SRX121241,SRX134721 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.10.AllAg.hESC_derived_neural_cells.bed ...

  18. File list: Unc.PSC.50.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.50.AllAg.mESC_derived_neural_cells mm9 Unclassified Pluripotent stem cell mESC derived neural...,SRX213761,SRX213757 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.50.AllAg.mESC_derived_neural_cells.bed ...

  19. File list: DNS.PSC.20.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.mESC_derived_neural_cells mm9 DNase-seq Pluripotent stem cell mESC derived neural... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.20.AllAg.mESC_derived_neural_cells.bed ...

  20. File list: Pol.PSC.50.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.mESC_derived_neural_cells mm9 RNA polymerase Pluripotent stem cell mESC derived neural...RX213760,SRX213764 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.50.AllAg.mESC_derived_neural_cells.bed ...

  1. File list: DNS.PSC.50.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.50.AllAg.mESC_derived_neural_cells mm9 DNase-seq Pluripotent stem cell mESC derived neural... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.50.AllAg.mESC_derived_neural_cells.bed ...

  2. File list: NoD.PSC.20.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.20.AllAg.mESC_derived_neural_cells mm9 No description Pluripotent stem cell mESC derived neural... cells SRX440731,SRX440736 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.20.AllAg.mESC_derived_neural_cells.bed ...

  3. File list: DNS.PSC.05.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.hESC_derived_neural_cells hg19 DNase-seq Pluripotent stem cell hESC derived neural... cells SRX121241,SRX134721 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.05.AllAg.hESC_derived_neural_cells.bed ...

  4. File list: Oth.PSC.20.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.AllAg.mESC_derived_neural_cells mm9 TFs and others Pluripotent stem cell mESC derived neural...52996,SRX213763,SRX213758 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.AllAg.mESC_derived_neural_cells.bed ...

  5. File list: Unc.PSC.20.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.20.AllAg.hESC_derived_neural_crests hg19 Unclassified Pluripotent stem cell hESC derived neural... crests SRX059366 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.20.AllAg.hESC_derived_neural_crests.bed ...

  6. File list: Pol.PSC.20.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.hESC_derived_neural_crests hg19 RNA polymerase Pluripotent stem cell hESC derived neural... crests http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.20.AllAg.hESC_derived_neural_crests.bed ...

  7. File list: Oth.PSC.05.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.05.AllAg.mESC_derived_neural_cells mm9 TFs and others Pluripotent stem cell mESC derived neural...10563,SRX213763,SRX352996 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.05.AllAg.mESC_derived_neural_cells.bed ...

  8. File list: NoD.PSC.50.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.mESC_derived_neural_cells mm9 No description Pluripotent stem cell mESC derived neural... cells SRX440736,SRX440731 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.50.AllAg.mESC_derived_neural_cells.bed ...

  9. File list: InP.PSC.50.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.50.AllAg.hESC_derived_neural_crests hg19 Input control Pluripotent stem cell hESC derived neural... crests SRX1091573,SRX059369,SRX059361 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.50.AllAg.hESC_derived_neural_crests.bed ...

  10. File list: InP.PSC.05.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.05.AllAg.hESC_derived_neural_crests hg19 Input control Pluripotent stem cell hESC derived neural... crests SRX1091573,SRX059369,SRX059361 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.05.AllAg.hESC_derived_neural_crests.bed ...

  11. File list: DNS.PSC.20.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.hESC_derived_neural_cells hg19 DNase-seq Pluripotent stem cell hESC derived neural... cells SRX121241,SRX134721 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.20.AllAg.hESC_derived_neural_cells.bed ...

  12. File list: ALL.PSC.05.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.hESC_derived_neural_crests hg19 All antigens Pluripotent stem cell hESC derived neural...RX059366,SRX059364 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.05.AllAg.hESC_derived_neural_crests.bed ...

  13. File list: NoD.PSC.50.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.hESC_derived_neural_cells hg19 No description Pluripotent stem cell hESC derived neural...edbc.jp/kyushu-u/hg19/assembled/NoD.PSC.50.AllAg.hESC_derived_neural_cells.bed ...

  14. File list: InP.PSC.10.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.10.AllAg.hESC_derived_neural_cells hg19 Input control Pluripotent stem cell hESC derived neural...RX698183,SRX729701,SRX729711 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.10.AllAg.hESC_derived_neural_cells.bed ...

  15. File list: Oth.PSC.05.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.05.AllAg.hESC_derived_neural_cells hg19 TFs and others Pluripotent stem cell hESC derived neural...http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.PSC.05.AllAg.hESC_derived_neural_cells.bed ...

  16. File list: His.PSC.50.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.50.AllAg.hESC_derived_neural_cells hg19 Histone Pluripotent stem cell hESC derived neural...692,SRX729710,SRX729684,SRX729689,SRX729699,SRX729704,SRX729694 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.50.AllAg.hESC_derived_neural_cells.bed ...

  17. File list: NoD.PSC.10.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.10.AllAg.mESC_derived_neural_cells mm9 No description Pluripotent stem cell mESC derived neural... cells SRX440736,SRX440731 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.10.AllAg.mESC_derived_neural_cells.bed ...

  18. File list: Pol.PSC.20.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.hESC_derived_neural_cells hg19 RNA polymerase Pluripotent stem cell hESC derived neural... cells SRX190259 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.20.AllAg.hESC_derived_neural_cells.bed ...

  19. File list: His.PSC.50.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.50.AllAg.hESC_derived_neural_crests hg19 Histone Pluripotent stem cell hESC derived neural...30,SRX059362,SRX1091539,SRX059364 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.50.AllAg.hESC_derived_neural_crests.bed ...

  20. File list: ALL.PSC.10.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.hESC_derived_neural_crests hg19 All antigens Pluripotent stem cell hESC derived neural...X059364,SRX1091530 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.10.AllAg.hESC_derived_neural_crests.bed ...

  1. File list: His.PSC.05.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.hESC_derived_neural_cells hg19 Histone Pluripotent stem cell hESC derived neural...707,SRX027494,SRX698181,SRX729681,SRX729682,SRX729698,SRX729709 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.05.AllAg.hESC_derived_neural_cells.bed ...

  2. File list: ALL.PSC.50.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.50.AllAg.hESC_derived_neural_crests hg19 All antigens Pluripotent stem cell hESC derived neural...X1091539,SRX059364 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.50.AllAg.hESC_derived_neural_crests.bed ...

  3. File list: Oth.PSC.50.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.50.AllAg.hESC_derived_neural_cells hg19 TFs and others Pluripotent stem cell hESC derived neural...http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.PSC.50.AllAg.hESC_derived_neural_cells.bed ...

  4. File list: Unc.PSC.20.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.20.AllAg.mESC_derived_neural_cells mm9 Unclassified Pluripotent stem cell mESC derived neural...,SRX213761,SRX213757 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.20.AllAg.mESC_derived_neural_cells.bed ...

  5. File list: Unc.PSC.05.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.hESC_derived_neural_crests hg19 Unclassified Pluripotent stem cell hESC derived neural... crests SRX059366 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.05.AllAg.hESC_derived_neural_crests.bed ...

  6. File list: His.PSC.20.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.hESC_derived_neural_cells hg19 Histone Pluripotent stem cell hESC derived neural...710,SRX729692,SRX729688,SRX729689,SRX729698,SRX729709,SRX729699 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.20.AllAg.hESC_derived_neural_cells.bed ...

  7. File list: DNS.PSC.50.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.50.AllAg.hESC_derived_neural_cells hg19 DNase-seq Pluripotent stem cell hESC derived neural... cells SRX121241,SRX134721 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.50.AllAg.hESC_derived_neural_cells.bed ...

  8. File list: Pol.PSC.50.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.hESC_derived_neural_cells hg19 RNA polymerase Pluripotent stem cell hESC derived neural... cells SRX190259 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.50.AllAg.hESC_derived_neural_cells.bed ...

  9. File list: NoD.PSC.05.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.hESC_derived_neural_crests hg19 No description Pluripotent stem cell hESC derived neural... crests http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.PSC.05.AllAg.hESC_derived_neural_crests.bed ...

  10. File list: Oth.PSC.50.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.50.AllAg.hESC_derived_neural_crests hg19 TFs and others Pluripotent stem cell hESC derived neural...X1091550,SRX059360,SRX1091547,SRX059367,SRX059368 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.PSC.50.AllAg.hESC_derived_neural_crests.bed ...

  11. File list: NoD.PSC.05.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.mESC_derived_neural_cells mm9 No description Pluripotent stem cell mESC derived neural... cells SRX440731,SRX440736 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.05.AllAg.mESC_derived_neural_cells.bed ...

  12. File list: His.PSC.20.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.hESC_derived_neural_crests hg19 Histone Pluripotent stem cell hESC derived neural...30,SRX059362,SRX1091539,SRX059364 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.20.AllAg.hESC_derived_neural_crests.bed ...

  13. File list: NoD.PSC.10.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.10.AllAg.hESC_derived_neural_cells hg19 No description Pluripotent stem cell hESC derived neural...edbc.jp/kyushu-u/hg19/assembled/NoD.PSC.10.AllAg.hESC_derived_neural_cells.bed ...

  14. File list: Oth.PSC.10.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.AllAg.mESC_derived_neural_cells mm9 TFs and others Pluripotent stem cell mESC derived neural...13763,SRX213758,SRX352996 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.10.AllAg.mESC_derived_neural_cells.bed ...

  15. File list: NoD.PSC.10.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.10.AllAg.hESC_derived_neural_crests hg19 No description Pluripotent stem cell hESC derived neural... crests http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.PSC.10.AllAg.hESC_derived_neural_crests.bed ...

  16. File list: His.PSC.10.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.hESC_derived_neural_crests hg19 Histone Pluripotent stem cell hESC derived neural...3,SRX1091531,SRX059364,SRX1091530 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.10.AllAg.hESC_derived_neural_crests.bed ...

  17. File list: Pol.PSC.05.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.hESC_derived_neural_crests hg19 RNA polymerase Pluripotent stem cell hESC derived neural... crests http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.05.AllAg.hESC_derived_neural_crests.bed ...

  18. File list: InP.PSC.50.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.50.AllAg.mESC_derived_mesodermal_cells mm9 Input control Pluripotent stem cell mESC derived meso...://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.50.AllAg.mESC_derived_mesodermal_cells.bed ...

  19. File list: NoD.PSC.05.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.mESC_derived_mesodermal_cells mm9 No description Pluripotent stem cell mESC derived meso...dermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.05.AllAg.mESC_derived_mesodermal_cells.bed ...

  20. File list: Oth.PSC.05.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.05.AllAg.mESC_derived_mesodermal_cells mm9 TFs and others Pluripotent stem cell mESC derived meso...282665,SRX504214,SRX504215,SRX282666,SRX282670,SRX757574,SRX504218 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.05.AllAg.mESC_derived_mesodermal_cells.bed ...

  1. File list: Unc.PSC.10.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.10.AllAg.mESC_derived_mesodermal_cells mm9 Unclassified Pluripotent stem cell mESC derived meso...cedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.10.AllAg.mESC_derived_mesodermal_cells.bed ...

  2. File list: NoD.PSC.10.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.10.AllAg.mESC_derived_mesodermal_cells mm9 No description Pluripotent stem cell mESC derived meso...dermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.10.AllAg.mESC_derived_mesodermal_cells.bed ...

  3. File list: Pol.PSC.20.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.mESC_derived_mesodermal_cells mm9 RNA polymerase Pluripotent stem cell mESC derived meso...dermal cells SRX305931,SRX305930 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.20.AllAg.mESC_derived_mesodermal_cells.bed ...

  4. File list: Pol.PSC.05.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.mESC_derived_mesodermal_cells mm9 RNA polymerase Pluripotent stem cell mESC derived meso...dermal cells SRX305930,SRX305931 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.05.AllAg.mESC_derived_mesodermal_cells.bed ...

  5. File list: NoD.PSC.20.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.20.AllAg.mESC_derived_mesodermal_cells mm9 No description Pluripotent stem cell mESC derived meso...dermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.20.AllAg.mESC_derived_mesodermal_cells.bed ...

  6. File list: Pol.PSC.10.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.mESC_derived_mesodermal_cells mm9 RNA polymerase Pluripotent stem cell mESC derived meso...dermal cells SRX305931,SRX305930 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.10.AllAg.mESC_derived_mesodermal_cells.bed ...

  7. File list: Pol.PSC.50.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.mESC_derived_mesodermal_cells mm9 RNA polymerase Pluripotent stem cell mESC derived meso...dermal cells SRX305931,SRX305930 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.50.AllAg.mESC_derived_mesodermal_cells.bed ...

  8. File list: DNS.PSC.10.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.mESC_derived_mesodermal_cells mm9 DNase-seq Pluripotent stem cell mESC derived meso...dermal cells SRX404483,SRX404484 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.10.AllAg.mESC_derived_mesodermal_cells.bed ...

  9. File list: ALL.PSC.05.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.mESC_derived_mesodermal_cells mm9 All antigens Pluripotent stem cell mESC derived meso...RX282671,SRX504221,SRX504218,SRX504220,SRX122635,SRX122639 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.05.AllAg.mESC_derived_mesodermal_cells.bed ...

  10. File list: His.PSC.05.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.mESC_derived_mesodermal_cells mm9 Histone Pluripotent stem cell mESC derived meso...,SRX504210,SRX305913,SRX122648,SRX122635,SRX122639 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.05.AllAg.mESC_derived_mesodermal_cells.bed ...

  11. File list: InP.PSC.20.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.20.AllAg.mESC_derived_mesodermal_cells mm9 Input control Pluripotent stem cell mESC derived meso...://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.20.AllAg.mESC_derived_mesodermal_cells.bed ...

  12. File list: InP.PSC.05.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.05.AllAg.mESC_derived_mesodermal_cells mm9 Input control Pluripotent stem cell mESC derived meso...://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.05.AllAg.mESC_derived_mesodermal_cells.bed ...

  13. File list: ALL.PSC.10.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.mESC_derived_mesodermal_cells mm9 All antigens Pluripotent stem cell mESC derived meso...RX504220,SRX282671,SRX122648,SRX122635,SRX122647,SRX122639 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.10.AllAg.mESC_derived_mesodermal_cells.bed ...

  14. File list: Oth.PSC.20.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.AllAg.mESC_derived_mesodermal_cells mm9 TFs and others Pluripotent stem cell mESC derived meso...470250,SRX282670,SRX757574,SRX504214,SRX504219,SRX504218,SRX504215 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.AllAg.mESC_derived_mesodermal_cells.bed ...

  15. File list: Unc.PSC.20.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.20.AllAg.mESC_derived_mesodermal_cells mm9 Unclassified Pluripotent stem cell mESC derived meso...cedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.20.AllAg.mESC_derived_mesodermal_cells.bed ...

  16. File list: ALL.PSC.50.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.50.AllAg.mESC_derived_mesodermal_cells mm9 All antigens Pluripotent stem cell mESC derived meso...RX122645,SRX122639,SRX122646,SRX122637,SRX122635,SRX504223 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.50.AllAg.mESC_derived_mesodermal_cells.bed ...

  17. File list: Oth.PSC.10.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.AllAg.mESC_derived_mesodermal_cells mm9 TFs and others Pluripotent stem cell mESC derived meso...282665,SRX504215,SRX282666,SRX282670,SRX504219,SRX757574,SRX504218 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.10.AllAg.mESC_derived_mesodermal_cells.bed ...

  18. File list: InP.PSC.10.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.10.AllAg.mESC_derived_mesodermal_cells mm9 Input control Pluripotent stem cell mESC derived meso...://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.10.AllAg.mESC_derived_mesodermal_cells.bed ...

  19. File list: NoD.PSC.50.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.mESC_derived_mesodermal_cells mm9 No description Pluripotent stem cell mESC derived meso...dermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.50.AllAg.mESC_derived_mesodermal_cells.bed ...

  20. File list: Unc.PSC.50.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.50.AllAg.mESC_derived_mesodermal_cells mm9 Unclassified Pluripotent stem cell mESC derived meso...cedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.50.AllAg.mESC_derived_mesodermal_cells.bed ...

  1. File list: His.PSC.50.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.50.AllAg.mESC_derived_mesodermal_cells mm9 Histone Pluripotent stem cell mESC derived meso...,SRX122639,SRX122646,SRX122637,SRX122635,SRX504223 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.50.AllAg.mESC_derived_mesodermal_cells.bed ...

  2. File list: Oth.PSC.50.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.50.AllAg.mESC_derived_mesodermal_cells mm9 TFs and others Pluripotent stem cell mESC derived meso...282665,SRX282670,SRX504214,SRX504219,SRX504218,SRX504215,SRX757574 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.50.AllAg.mESC_derived_mesodermal_cells.bed ...

  3. File list: ALL.PSC.20.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.20.AllAg.mESC_derived_mesodermal_cells mm9 All antigens Pluripotent stem cell mESC derived meso...RX122646,SRX122647,SRX122638,SRX122648,SRX122639,SRX122635 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.20.AllAg.mESC_derived_mesodermal_cells.bed ...

  4. File list: His.PSC.10.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.mESC_derived_mesodermal_cells mm9 Histone Pluripotent stem cell mESC derived meso...,SRX305914,SRX122648,SRX122635,SRX122647,SRX122639 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.10.AllAg.mESC_derived_mesodermal_cells.bed ...

  5. File list: Unc.PSC.05.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.mESC_derived_mesodermal_cells mm9 Unclassified Pluripotent stem cell mESC derived meso...cedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.05.AllAg.mESC_derived_mesodermal_cells.bed ...

  6. File list: DNS.PSC.20.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.mESC_derived_mesodermal_cells mm9 DNase-seq Pluripotent stem cell mESC derived meso...dermal cells SRX404483,SRX404484 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.20.AllAg.mESC_derived_mesodermal_cells.bed ...

  7. File list: His.PSC.20.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.mESC_derived_mesodermal_cells mm9 Histone Pluripotent stem cell mESC derived meso...,SRX122647,SRX122638,SRX122648,SRX122639,SRX122635 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.20.AllAg.mESC_derived_mesodermal_cells.bed ...

  8. File list: DNS.PSC.05.AllAg.mESC_derived_mesodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.mESC_derived_mesodermal_cells mm9 DNase-seq Pluripotent stem cell mESC derived meso...dermal cells SRX404483,SRX404484 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.05.AllAg.mESC_derived_mesodermal_cells.bed ...

  9. File list: Pol.PSC.50.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.mESC_derived_epiblast-like_cells mm9 RNA polymerase Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.50.AllAg.mESC_derived_epiblast-like_cells.bed ...

  10. File list: His.PSC.20.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.mESC_derived_epiblast-like_cells mm9 Histone Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.20.AllAg.mESC_derived_epiblast-like_cells.bed ...

  11. File list: NoD.PSC.10.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.10.AllAg.mESC_derived_epiblast-like_cells mm9 No description Pluripotent stem cell mESC derived epib...41,SRX336249,SRX336248 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.10.AllAg.mESC_derived_epiblast-like_cells.bed ...

  12. File list: Pol.PSC.05.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.mESC_derived_epiblast-like_cells mm9 RNA polymerase Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.05.AllAg.mESC_derived_epiblast-like_cells.bed ...

  13. File list: Oth.PSC.20.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.AllAg.mESC_derived_epiblast-like_cells mm9 TFs and others Pluripotent stem cell mESC derived epib...last-like cells SRX1082043,SRX1082042 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.AllAg.mESC_derived_epiblast-like_cells.bed ...

  14. File list: Unc.PSC.05.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.mESC_derived_epiblast-like_cells mm9 Unclassified Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.05.AllAg.mESC_derived_epiblast-like_cells.bed ...

  15. File list: NoD.PSC.05.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.mESC_derived_epiblast-like_cells mm9 No description Pluripotent stem cell mESC derived epib...48,SRX336242,SRX336241 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.05.AllAg.mESC_derived_epiblast-like_cells.bed ...

  16. File list: Unc.PSC.50.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.50.AllAg.mESC_derived_epiblast-like_cells mm9 Unclassified Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.50.AllAg.mESC_derived_epiblast-like_cells.bed ...

  17. File list: His.PSC.10.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.mESC_derived_epiblast-like_cells mm9 Histone Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.10.AllAg.mESC_derived_epiblast-like_cells.bed ...

  18. File list: InP.PSC.20.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.20.AllAg.mESC_derived_epiblast-like_cells mm9 Input control Pluripotent stem cell mESC derived epib...last-like cells SRX1082041 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.20.AllAg.mESC_derived_epiblast-like_cells.bed ...

  19. File list: DNS.PSC.50.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.50.AllAg.mESC_derived_epiblast-like_cells mm9 DNase-seq Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.50.AllAg.mESC_derived_epiblast-like_cells.bed ...

  20. File list: DNS.PSC.10.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.mESC_derived_epiblast-like_cells mm9 DNase-seq Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.10.AllAg.mESC_derived_epiblast-like_cells.bed ...

  1. File list: Pol.PSC.20.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.mESC_derived_epiblast-like_cells mm9 RNA polymerase Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.20.AllAg.mESC_derived_epiblast-like_cells.bed ...

  2. File list: InP.PSC.10.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.10.AllAg.mESC_derived_epiblast-like_cells mm9 Input control Pluripotent stem cell mESC derived epib...last-like cells SRX1082041 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.10.AllAg.mESC_derived_epiblast-like_cells.bed ...

  3. File list: Unc.PSC.10.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.10.AllAg.mESC_derived_epiblast-like_cells mm9 Unclassified Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.10.AllAg.mESC_derived_epiblast-like_cells.bed ...

  4. File list: Oth.PSC.50.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.50.AllAg.mESC_derived_epiblast-like_cells mm9 TFs and others Pluripotent stem cell mESC derived epib...last-like cells SRX1082043,SRX1082042 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.50.AllAg.mESC_derived_epiblast-like_cells.bed ...

  5. File list: ALL.PSC.50.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.50.AllAg.mESC_derived_epiblast-like_cells mm9 All antigens Pluripotent stem cell mESC derived epib...1,SRX1082043,SRX1082042,SRX336247,SRX336248,SRX336249 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.50.AllAg.mESC_derived_epiblast-like_cells.bed ...

  6. File list: Oth.PSC.05.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.05.AllAg.mESC_derived_epiblast-like_cells mm9 TFs and others Pluripotent stem cell mESC derived epib...last-like cells SRX1082042,SRX1082043 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.05.AllAg.mESC_derived_epiblast-like_cells.bed ...

  7. File list: DNS.PSC.20.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.mESC_derived_epiblast-like_cells mm9 DNase-seq Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.20.AllAg.mESC_derived_epiblast-like_cells.bed ...

  8. File list: InP.PSC.05.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.05.AllAg.mESC_derived_epiblast-like_cells mm9 Input control Pluripotent stem cell mESC derived epib...last-like cells SRX1082041 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.05.AllAg.mESC_derived_epiblast-like_cells.bed ...

  9. File list: DNS.PSC.05.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.mESC_derived_epiblast-like_cells mm9 DNase-seq Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.05.AllAg.mESC_derived_epiblast-like_cells.bed ...

  10. File list: Unc.PSC.20.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.20.AllAg.mESC_derived_epiblast-like_cells mm9 Unclassified Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.20.AllAg.mESC_derived_epiblast-like_cells.bed ...

  11. File list: Pol.PSC.10.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.mESC_derived_epiblast-like_cells mm9 RNA polymerase Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.10.AllAg.mESC_derived_epiblast-like_cells.bed ...

  12. File list: NoD.PSC.50.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.mESC_derived_epiblast-like_cells mm9 No description Pluripotent stem cell mESC derived epib...47,SRX336248,SRX336249 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.50.AllAg.mESC_derived_epiblast-like_cells.bed ...

  13. File list: Oth.PSC.10.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.AllAg.mESC_derived_epiblast-like_cells mm9 TFs and others Pluripotent stem cell mESC derived epib...last-like cells SRX1082043,SRX1082042 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.10.AllAg.mESC_derived_epiblast-like_cells.bed ...

  14. File list: His.PSC.05.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.mESC_derived_epiblast-like_cells mm9 Histone Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.05.AllAg.mESC_derived_epiblast-like_cells.bed ...

  15. File list: NoD.PSC.20.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.20.AllAg.mESC_derived_epiblast-like_cells mm9 No description Pluripotent stem cell mESC derived epib...47,SRX336248,SRX336249 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.20.AllAg.mESC_derived_epiblast-like_cells.bed ...

  16. File list: His.PSC.50.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.50.AllAg.mESC_derived_epiblast-like_cells mm9 Histone Pluripotent stem cell mESC derived epib...last-like cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.50.AllAg.mESC_derived_epiblast-like_cells.bed ...

  17. File list: ALL.PSC.05.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.mESC_derived_epiblast-like_cells mm9 All antigens Pluripotent stem cell mESC derived epib...,SRX1082042,SRX336242,SRX336241,SRX1082041,SRX1082043 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.05.AllAg.mESC_derived_epiblast-like_cells.bed ...

  18. File list: InP.PSC.50.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.50.AllAg.mESC_derived_epiblast-like_cells mm9 Input control Pluripotent stem cell mESC derived epib...last-like cells SRX1082041 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.50.AllAg.mESC_derived_epiblast-like_cells.bed ...

  19. File list: ALL.PSC.10.AllAg.mESC_derived_epiblast-like_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.mESC_derived_epiblast-like_cells mm9 All antigens Pluripotent stem cell mESC derived epib...3,SRX1082041,SRX336241,SRX1082042,SRX336249,SRX336248 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.10.AllAg.mESC_derived_epiblast-like_cells.bed ...

  20. Oral vaccination of channel catfish against enteric septicemia of catfish (ESC) using a live attenuated Edwardsiella ictaluri isolate

    Science.gov (United States)

    Enteric septicemia of catfish (ESC), caused by Edwardsiella ictaluri, is the most problematic bacterial disease affecting catfish aquaculture in the southeastern United States. Efforts to develop an effective ESC vaccine have had limited industrial success. In commercial settings, ESC vaccines are t...

  1. File list: Pol.PSC.50.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.hESC_derived_mesendodermal_cells hg19 RNA polymerase Pluripotent s...edbc.jp/kyushu-u/hg19/assembled/Pol.PSC.50.AllAg.hESC_derived_mesendodermal_cells.bed ... ...tem cell hESC derived mesendodermal cells SRX702060,SRX149642,SRX702059,SRX702061 http://dbarchive.bioscienc

  2. File list: Unc.PSC.05.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.hESC_derived_mesendodermal_cells hg19 Unclassified Pluripotent stem cell hESC derived mesen...dodermal cells SRX764814,SRX378282,SRX378283 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.05.AllAg.hESC_derived_mesendodermal_cells.bed ...

  3. File list: His.PSC.10.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.hESC_derived_mesendodermal_cells hg19 Histone Pluripotent stem cell hESC derived mesend...,SRX702007,SRX702017 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.10.AllAg.hESC_derived_mesendodermal_cells.bed ...

  4. File list: ALL.PSC.10.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.hESC_derived_mesendodermal_cells hg19 All antigens Pluripotent stem cell hESC derived mesen...01985,SRX702128,SRX702094,SRX702102 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.10.AllAg.hESC_derived_mesendodermal_cells.bed ...

  5. File list: DNS.PSC.10.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.hESC_derived_mesendodermal_cells hg19 DNase-seq Pluripotent stem cell hESC derived mesend...chive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.10.AllAg.hESC_derived_mesendodermal_cells.bed ...

  6. File list: His.PSC.05.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.hESC_derived_mesendodermal_cells hg19 Histone Pluripotent stem cell hESC derived mesend...,SRX702016,SRX702017 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.05.AllAg.hESC_derived_mesendodermal_cells.bed ...

  7. File list: His.PSC.50.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.50.AllAg.hESC_derived_mesendodermal_cells hg19 Histone Pluripotent stem cell hESC derived mesend...,SRX702008,SRX702007 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.50.AllAg.hESC_derived_mesendodermal_cells.bed ...

  8. File list: Unc.PSC.10.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.10.AllAg.hESC_derived_mesendodermal_cells hg19 Unclassified Pluripotent stem cell hESC derived mesen...dodermal cells SRX764814,SRX378282,SRX378283 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.10.AllAg.hESC_derived_mesendodermal_cells.bed ...

  9. File list: Pol.PSC.20.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.hESC_derived_mesendodermal_cells hg19 RNA polymerase Pluripotent s...edbc.jp/kyushu-u/hg19/assembled/Pol.PSC.20.AllAg.hESC_derived_mesendodermal_cells.bed ... ...tem cell hESC derived mesendodermal cells SRX702060,SRX149642,SRX702059,SRX702061 http://dbarchive.bioscienc

  10. File list: DNS.PSC.05.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.hESC_derived_mesendodermal_cells hg19 DNase-seq Pluripotent stem cell hESC derived mesend...chive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.05.AllAg.hESC_derived_mesendodermal_cells.bed ...

  11. File list: ALL.PSC.05.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.hESC_derived_mesendodermal_cells hg19 All antigens Pluripotent stem cell hESC derived mesen...hive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.05.AllAg.hESC_derived_mesendodermal_cells.bed ...

  12. File list: Pol.PSC.10.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.hESC_derived_mesendodermal_cells hg19 RNA polymerase Pluripotent s...edbc.jp/kyushu-u/hg19/assembled/Pol.PSC.10.AllAg.hESC_derived_mesendodermal_cells.bed ... ...tem cell hESC derived mesendodermal cells SRX702060,SRX702061,SRX149642,SRX702059 http://dbarchive.bioscienc

  13. File list: DNS.PSC.50.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.50.AllAg.hESC_derived_mesendodermal_cells hg19 DNase-seq Pluripotent stem cell hESC derived mesend...chive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.50.AllAg.hESC_derived_mesendodermal_cells.bed ...

  14. File list: Unc.PSC.50.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.50.AllAg.hESC_derived_mesendodermal_cells hg19 Unclassified Pluripotent stem cell hESC derived mesen...dodermal cells SRX378282,SRX378283,SRX764814 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.50.AllAg.hESC_derived_mesendodermal_cells.bed ...

  15. File list: Pol.PSC.05.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.hESC_derived_mesendodermal_cells hg19 RNA polymerase Pluripotent s...edbc.jp/kyushu-u/hg19/assembled/Pol.PSC.05.AllAg.hESC_derived_mesendodermal_cells.bed ... ...tem cell hESC derived mesendodermal cells SRX702060,SRX702061,SRX149642,SRX702059 http://dbarchive.bioscienc

  16. File list: ALL.PSC.50.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.50.AllAg.hESC_derived_mesendodermal_cells hg19 All antigens Pluripotent stem cell hESC derived mesen...02105 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.50.AllAg.hESC_derived_mesendodermal_cells.bed ...

  17. File list: His.PSC.20.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.hESC_derived_mesendodermal_cells hg19 Histone Pluripotent stem cell hESC derived mesend...,SRX702009,SRX702008 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.20.AllAg.hESC_derived_mesendodermal_cells.bed ...

  18. File list: DNS.PSC.20.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.hESC_derived_mesendodermal_cells hg19 DNase-seq Pluripotent stem cell hESC derived mesend...chive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.PSC.20.AllAg.hESC_derived_mesendodermal_cells.bed ...

  19. File list: ALL.PSC.20.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.20.AllAg.hESC_derived_mesendodermal_cells hg19 All antigens Pluripotent stem cell hESC derived mesen...02128 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.20.AllAg.hESC_derived_mesendodermal_cells.bed ...

  20. File list: Unc.PSC.20.AllAg.hESC_derived_mesendodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.20.AllAg.hESC_derived_mesendodermal_cells hg19 Unclassified Pluripotent stem cell hESC derived mesen...dodermal cells SRX378282,SRX378283,SRX764814 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.20.AllAg.hESC_derived_mesendodermal_cells.bed ...

  1. File list: Pol.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor mm9 RNA polymerase Pluripot...ent stem cell mESC derived haematopoietic progenitor http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  2. File list: NoD.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor mm9 No description Pluripot...ent stem cell mESC derived haematopoietic progenitor http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  3. File list: Pol.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor mm9 RNA polymerase Pluripot...ent stem cell mESC derived haematopoietic progenitor http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  4. File list: His.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor mm9 Histone Pluripotent stem cell mESC derived hae...matopoietic progenitor SRX282672,SRX528335 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  5. File list: Oth.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor mm9 TFs and others Pluripot...ent stem cell mESC derived haematopoietic progenitor SRX310196,SRX825828,SRX825829,SRX021436,SRX310197,SRX82...iencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  6. File list: DNS.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor mm9 DNase-seq Pluripotent stem cell mESC derived hae...matopoietic progenitor http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  7. File list: Oth.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor mm9 TFs and others Pluripot...ent stem cell mESC derived haematopoietic progenitor SRX825828,SRX310197,SRX825829,SRX310196,SRX378972,SRX02...iencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  8. File list: NoD.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor mm9 No description Pluripot...ent stem cell mESC derived haematopoietic progenitor http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  9. File list: Oth.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor mm9 TFs and others Pluripot...ent stem cell mESC derived haematopoietic progenitor SRX825828,SRX310197,SRX310196,SRX825829,SRX021436,SRX37...iencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  10. File list: Pol.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor mm9 RNA polymerase Pluripot...ent stem cell mESC derived haematopoietic progenitor http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  11. File list: DNS.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor mm9 DNase-seq Pluripotent stem cell mESC derived hae...matopoietic progenitor http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  12. File list: DNS.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor mm9 DNase-seq Pluripotent stem cell mESC derived hae...matopoietic progenitor http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  13. File list: Pol.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor mm9 RNA polymerase Pluripot...ent stem cell mESC derived haematopoietic progenitor http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.10.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  14. File list: NoD.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor mm9 No description Pluripot...ent stem cell mESC derived haematopoietic progenitor http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  15. File list: Oth.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor mm9 TFs and others Pluripot...ent stem cell mESC derived haematopoietic progenitor SRX310196,SRX825828,SRX825829,SRX021436,SRX310197,SRX37...iencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.05.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  16. File list: His.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor mm9 Histone Pluripotent stem cell mESC derived hae...matopoietic progenitor SRX282672,SRX528335 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.20.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  17. File list: NoD.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor mm9 No description Pluripot...ent stem cell mESC derived haematopoietic progenitor http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.50.AllAg.mESC_derived_haematopoietic_progenitor.bed ...

  18. File list: Pol.PSC.10.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.hESC_derived_neural_cells hg19 RNA polymerase Pluripotent stem cell hESC derived neural... cells SRX190259 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.PSC.10.AllAg.hESC_derived_neural_cells.bed ...

  19. File list: Pol.PSC.05.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.mESC_derived_neural_cells mm9 RNA polymerase Pluripotent stem cell mESC derived neural...RX213764,SRX213760 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.05.AllAg.mESC_derived_neural_cells.bed ...

  20. File list: ALL.PSC.05.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.hESC_derived_neural_cells hg19 All antigens Pluripotent stem cell hESC derived neural...RX729682,SRX729698,SRX729709 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.05.AllAg.hESC_derived_neural_cells.bed ...

  1. File list: NoD.PSC.20.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.20.AllAg.hESC_derived_neural_cells hg19 No description Pluripotent stem cell hESC derived neural...edbc.jp/kyushu-u/hg19/assembled/NoD.PSC.20.AllAg.hESC_derived_neural_cells.bed ...

  2. File list: InP.PSC.05.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.05.AllAg.hESC_derived_neural_cells hg19 Input control Pluripotent stem cell hESC derived neural...RX698183,SRX729711,SRX729701 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.05.AllAg.hESC_derived_neural_cells.bed ...

  3. File list: Unc.PSC.05.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.hESC_derived_neural_cells hg19 Unclassified Pluripotent stem cell hESC derived neural... cells SRX378284 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.05.AllAg.hESC_derived_neural_cells.bed ...

  4. File list: NoD.PSC.20.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.20.AllAg.mESC_derived_endoodermal_cells mm9 No description Pluripotent stem cell mESC derived endo...RX149453,ERX149451 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.20.AllAg.mESC_derived_endoodermal_cells.bed ...

  5. File list: Unc.PSC.50.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.50.AllAg.mESC_derived_endoodermal_cells mm9 Unclassified Pluripotent stem cell mESC derived endo...odermal cells SRX501738 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.50.AllAg.mESC_derived_endoodermal_cells.bed ...

  6. File list: DNS.PSC.10.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.10.AllAg.mESC_derived_endoodermal_cells mm9 DNase-seq Pluripotent stem cell mESC derived endo...odermal cells SRX404486,SRX404485 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.10.AllAg.mESC_derived_endoodermal_cells.bed ...

  7. File list: ALL.PSC.10.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.10.AllAg.mESC_derived_endoodermal_cells mm9 All antigens Pluripotent stem cell mESC derived endo...RX501738 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.10.AllAg.mESC_derived_endoodermal_cells.bed ...

  8. File list: Unc.PSC.20.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.20.AllAg.mESC_derived_endoodermal_cells mm9 Unclassified Pluripotent stem cell mESC derived endo...odermal cells SRX501738 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.20.AllAg.mESC_derived_endoodermal_cells.bed ...

  9. File list: Oth.PSC.20.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.AllAg.mESC_derived_endoodermal_cells mm9 TFs and others Pluripotent stem cell mESC derived endo...ciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.AllAg.mESC_derived_endoodermal_cells.bed ...

  10. File list: Unc.PSC.05.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.mESC_derived_endoodermal_cells mm9 Unclassified Pluripotent stem cell mESC derived endo...odermal cells SRX501738 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.05.AllAg.mESC_derived_endoodermal_cells.bed ...

  11. File list: InP.PSC.50.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.50.AllAg.mESC_derived_endoodermal_cells mm9 Input control Pluripotent stem cell mESC derived endo...odermal cells SRX390400,SRX390398 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.50.AllAg.mESC_derived_endoodermal_cells.bed ...

  12. File list: Oth.PSC.05.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.05.AllAg.mESC_derived_endoodermal_cells mm9 TFs and others Pluripotent stem cell mESC derived endo...ciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.05.AllAg.mESC_derived_endoodermal_cells.bed ...

  13. File list: His.PSC.20.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.20.AllAg.mESC_derived_endoodermal_cells mm9 Histone Pluripotent stem cell mESC derived endo...odermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.20.AllAg.mESC_derived_endoodermal_cells.bed ...

  14. File list: InP.PSC.10.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.10.AllAg.mESC_derived_endoodermal_cells mm9 Input control Pluripotent stem cell mESC derived endo...odermal cells SRX390400,SRX390398 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.10.AllAg.mESC_derived_endoodermal_cells.bed ...

  15. File list: NoD.PSC.05.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.05.AllAg.mESC_derived_endoodermal_cells mm9 No description Pluripotent stem cell mESC derived endo...RX149444,ERX149451 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.05.AllAg.mESC_derived_endoodermal_cells.bed ...

  16. File list: Pol.PSC.05.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.05.AllAg.mESC_derived_endoodermal_cells mm9 RNA polymerase Pluripotent stem cell mESC derived endo...odermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.05.AllAg.mESC_derived_endoodermal_cells.bed ...

  17. File list: Oth.PSC.10.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.AllAg.mESC_derived_endoodermal_cells mm9 TFs and others Pluripotent stem cell mESC derived endo...ciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.10.AllAg.mESC_derived_endoodermal_cells.bed ...

  18. File list: NoD.PSC.10.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.10.AllAg.mESC_derived_endoodermal_cells mm9 No description Pluripotent stem cell mESC derived endo...RX149453,ERX149444 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.10.AllAg.mESC_derived_endoodermal_cells.bed ...

  19. File list: Pol.PSC.20.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.20.AllAg.mESC_derived_endoodermal_cells mm9 RNA polymerase Pluripotent stem cell mESC derived endo...odermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.20.AllAg.mESC_derived_endoodermal_cells.bed ...

  20. File list: ALL.PSC.50.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.50.AllAg.mESC_derived_endoodermal_cells mm9 All antigens Pluripotent stem cell mESC derived endo...RX501738 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.50.AllAg.mESC_derived_endoodermal_cells.bed ...

  1. File list: NoD.PSC.50.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.PSC.50.AllAg.mESC_derived_endoodermal_cells mm9 No description Pluripotent stem cell mESC derived endo...RX149453,ERX149451 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.PSC.50.AllAg.mESC_derived_endoodermal_cells.bed ...

  2. File list: ALL.PSC.20.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.20.AllAg.mESC_derived_endoodermal_cells mm9 All antigens Pluripotent stem cell mESC derived endo...RX501738 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.20.AllAg.mESC_derived_endoodermal_cells.bed ...

  3. File list: DNS.PSC.50.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.50.AllAg.mESC_derived_endoodermal_cells mm9 DNase-seq Pluripotent stem cell mESC derived endo...odermal cells SRX404485,SRX404486 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.50.AllAg.mESC_derived_endoodermal_cells.bed ...

  4. File list: Pol.PSC.50.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.50.AllAg.mESC_derived_endoodermal_cells mm9 RNA polymerase Pluripotent stem cell mESC derived endo...odermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.50.AllAg.mESC_derived_endoodermal_cells.bed ...

  5. File list: DNS.PSC.20.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.20.AllAg.mESC_derived_endoodermal_cells mm9 DNase-seq Pluripotent stem cell mESC derived endo...odermal cells SRX404485,SRX404486 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.20.AllAg.mESC_derived_endoodermal_cells.bed ...

  6. File list: Unc.PSC.10.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.10.AllAg.mESC_derived_endoodermal_cells mm9 Unclassified Pluripotent stem cell mESC derived endo...odermal cells SRX501738 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.10.AllAg.mESC_derived_endoodermal_cells.bed ...

  7. File list: InP.PSC.05.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.05.AllAg.mESC_derived_endoodermal_cells mm9 Input control Pluripotent stem cell mESC derived endo...odermal cells SRX390400,SRX390398 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.05.AllAg.mESC_derived_endoodermal_cells.bed ...

  8. File list: DNS.PSC.05.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.PSC.05.AllAg.mESC_derived_endoodermal_cells mm9 DNase-seq Pluripotent stem cell mESC derived endo...odermal cells SRX404485,SRX404486 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.PSC.05.AllAg.mESC_derived_endoodermal_cells.bed ...

  9. File list: His.PSC.05.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.mESC_derived_endoodermal_cells mm9 Histone Pluripotent stem cell mESC derived endo...odermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.05.AllAg.mESC_derived_endoodermal_cells.bed ...

  10. File list: ALL.PSC.05.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.05.AllAg.mESC_derived_endoodermal_cells mm9 All antigens Pluripotent stem cell mESC derived endo...RX501738 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.PSC.05.AllAg.mESC_derived_endoodermal_cells.bed ...

  11. File list: His.PSC.10.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.10.AllAg.mESC_derived_endoodermal_cells mm9 Histone Pluripotent stem cell mESC derived endo...odermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.PSC.10.AllAg.mESC_derived_endoodermal_cells.bed ...

  12. File list: InP.PSC.20.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.20.AllAg.mESC_derived_endoodermal_cells mm9 Input control Pluripotent stem cell mESC derived endo...odermal cells SRX390400,SRX390398 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.PSC.20.AllAg.mESC_derived_endoodermal_cells.bed ...

  13. File list: Pol.PSC.10.AllAg.mESC_derived_endoodermal_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.PSC.10.AllAg.mESC_derived_endoodermal_cells mm9 RNA polymerase Pluripotent stem cell mESC derived endo...odermal cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.PSC.10.AllAg.mESC_derived_endoodermal_cells.bed ...

  14. File list: ALL.PSC.20.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.20.AllAg.hESC_derived_neural_cells hg19 All antigens Pluripotent stem cell hESC derived neural...RX729698,SRX729709,SRX729699 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.20.AllAg.hESC_derived_neural_cells.bed ...

  15. File list: Oth.PSC.20.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.AllAg.hESC_derived_neural_cells hg19 TFs and others Pluripotent stem cell hESC derived neural...http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.PSC.20.AllAg.hESC_derived_neural_cells.bed ...

  16. File list: ALL.PSC.20.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.PSC.20.AllAg.hESC_derived_neural_crests hg19 All antigens Pluripotent stem cell hESC derived neural...X1091539,SRX059364 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.PSC.20.AllAg.hESC_derived_neural_crests.bed ...

  17. File list: Unc.PSC.05.AllAg.mESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.05.AllAg.mESC_derived_neural_cells mm9 Unclassified Pluripotent stem cell mESC derived neural...,SRX213761,SRX213757 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.PSC.05.AllAg.mESC_derived_neural_cells.bed ...

  18. File list: Unc.PSC.50.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.PSC.50.AllAg.hESC_derived_neural_crests hg19 Unclassified Pluripotent stem cell hESC derived neural... crests SRX059366 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Unc.PSC.50.AllAg.hESC_derived_neural_crests.bed ...

  19. File list: His.PSC.05.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.PSC.05.AllAg.hESC_derived_neural_crests hg19 Histone Pluripotent stem cell hESC derived neural...13,SRX1091515,SRX059363,SRX059364 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.PSC.05.AllAg.hESC_derived_neural_crests.bed ...

  20. File list: InP.PSC.20.AllAg.hESC_derived_neural_crests [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.20.AllAg.hESC_derived_neural_crests hg19 Input control Pluripotent stem cell hESC derived neural... crests SRX1091573,SRX059369,SRX059361 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.20.AllAg.hESC_derived_neural_crests.bed ...

  1. File list: InP.PSC.50.AllAg.hESC_derived_neural_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.PSC.50.AllAg.hESC_derived_neural_cells hg19 Input control Pluripotent stem cell hESC derived neural...RX698183,SRX326376,SRX027491 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.PSC.50.AllAg.hESC_derived_neural_cells.bed ...

  2. Geographical distribution of salmonella infected pig, cattle and sheep herds in Sweden 1993-2010

    Directory of Open Access Journals (Sweden)

    Skog Lars

    2011-10-01

    Full Text Available Abstract Background The Swedish salmonella control programme covers the entire production chain, from feed to food. All salmonella serotypes are notifiable. On average, less than 20 cases of salmonella in food-producing animals are reported every year. In some situations, the cases would be expected to cluster geographically. The aim of this study was to illustrate the geographic distribution of the salmonella cases detected in pigs, cattle and sheep. Methods Data on all herds with pigs, cattle and sheep found to be infected with salmonella during the time period from 1993 to 2010 were obtained from the Swedish Board of Agriculture. Using the ArcGIS software, various maps were produced of infected herds, stratified on animal species as well as salmonella serotype. Based on ocular inspection of all maps, some were collapsed and some used separately. Data were also examined for temporal trends. Results No geographical clustering was observed for ovine or porcine cases. Cattle herds infected with Salmonella Dublin were mainly located in the southeast region and cattle herds infected with Salmonella Typhimurium in the most southern part of the country. Some seasonal variation was seen in cattle, but available data was not sufficient for further analyses. Conclusions Analyses of data on salmonella infected herds revealed some spatial and temporal patterns for salmonella in cattle. However, despite using 18 years' of data, the number of infected herds was too low for any useful statistical analyses.

  3. Geographical distribution of salmonella infected pig, cattle and sheep herds in Sweden 1993-2010.

    Science.gov (United States)

    Lewerin, Susanna Sternberg; Skog, Lars; Frössling, Jenny; Wahlström, Helene

    2011-10-05

    The Swedish salmonella control programme covers the entire production chain, from feed to food. All salmonella serotypes are notifiable. On average, less than 20 cases of salmonella in food-producing animals are reported every year. In some situations, the cases would be expected to cluster geographically. The aim of this study was to illustrate the geographic distribution of the salmonella cases detected in pigs, cattle and sheep. Data on all herds with pigs, cattle and sheep found to be infected with salmonella during the time period from 1993 to 2010 were obtained from the Swedish Board of Agriculture. Using the ArcGIS software, various maps were produced of infected herds, stratified on animal species as well as salmonella serotype. Based on ocular inspection of all maps, some were collapsed and some used separately. Data were also examined for temporal trends. No geographical clustering was observed for ovine or porcine cases. Cattle herds infected with Salmonella Dublin were mainly located in the southeast region and cattle herds infected with Salmonella Typhimurium in the most southern part of the country. Some seasonal variation was seen in cattle, but available data was not sufficient for further analyses. Analyses of data on salmonella infected herds revealed some spatial and temporal patterns for salmonella in cattle. However, despite using 18 years' of data, the number of infected herds was too low for any useful statistical analyses.

  4. THE INFLUENCE OF SALMONELLA IN PIGS PRE-HARVEST ON SALMONELLA HUMAN HEALTH COSTS AND RISK FROM PORK

    OpenAIRE

    Gay Y. Miller; Liu, Xuanli; Paul E. McNamara; Barber, David A.

    2004-01-01

    Salmonellosis in people is a costly disease, much of it occurring because of food associated exposure. We develop a farm-to-fork model which estimates the pork associated Salmonella risk and human health costs. This analysis focuses on the components of the pork production chain up to the point of producing a chilled pork carcass. Sensitivity and scenario analysis show that changes that occur in Salmonella status during processing are substantially more important for human health risk and hav...

  5. Estimation of costs for control of Salmonella in high-risk feed materials and compound feed

    OpenAIRE

    Wierup, Martin; Widell, Stig

    2014-01-01

    Introduction: Feed is a potential and major source for introducing Salmonella into the animal-derived food chain. This is given special attention in the European Union (EU) efforts to minimize human food-borne Salmonella infections from animal-derived food. The objective of this study was to estimate the total extra cost for preventing Salmonella contamination of feed above those measures required to produce commercial feed according to EU regulation (EC) No 183/2005. The study was carried ou...

  6. Nutritional strategies to combat Salmonella in mono-gastric food animal production

    OpenAIRE

    Berge, Anna; Wierup, Martin

    2012-01-01

    Nutritional strategies to minimize Salmonella in food animal production are one of the key components in producing safer food. The current European approach is to use a farm-to-fork strategy, where each sector must implement measures to minimize and reduce Salmonella contamination. In the pre-harvest phase, this means that all available tools need to be used such as implementation of biosecurity measures, control of Salmonella infections in animals at the farm as well as in transport and trad...

  7. High yield derivation of enriched glutamatergic neurons from suspension-cultured mouse ESCs for neurotoxicology research

    Directory of Open Access Journals (Sweden)

    Hubbard Kyle S

    2012-10-01

    Full Text Available Abstract Background Recently, there has been a strong emphasis on identifying an in vitro model for neurotoxicity research that combines the biological relevance of primary neurons with the scalability, reproducibility and genetic tractability of continuous cell lines. Derived neurons should be homotypic, exhibit neuron-specific gene expression and morphology, form functioning synapses and consistently respond to neurotoxins in a fashion indistinguishable from primary neurons. However, efficient methods to produce neuronal populations that are suitable alternatives to primary neurons have not been available. Methods With the objective of developing a more facile, robust and efficient method to generate enriched glutamatergic neuronal cultures, we evaluated the neurogenic capacity of three mouse embryonic stem cell (ESC lines (R1, C57BL/6 and D3 adapted to feeder-independent suspension culture. Neurogenesis and neuronal maturation were characterized as a function of time in culture using immunological, genomic, morphological and functional metrics. The functional responses of ESNs to neurotropic toxins with distinctly different targets and mechanisms of toxicity, such as glutamate, α-latrotoxin (LTX, and botulinum neurotoxin (BoNT, were also evaluated. Results Suspension-adapted ESCs expressed markers of pluripotency through at least 30 passages, and differentiation produced 97×106 neural progenitor cells (NPCs per 10-cm dish. Greater than 99% of embryonic stem cell-derived neurons (ESNs expressed neuron-specific markers by 96 h after plating and rapidly developed complex axodendritic arbors and appropriate compartmentalization of neurotypic proteins. Expression profiling demonstrated the presence of transcripts necessary for neuronal function and confirmed that ESN populations were predominantly glutamatergic. Furthermore, ESNs were functionally receptive to all toxins with sensitivities and responses consistent with primary neurons

  8. The enhanced immune responses induced by Salmonella enteritidis ghosts loaded with Neisseria gonorrhoeae porB against Salmonella in mice.

    Science.gov (United States)

    Jiao, Hongmei; Yang, Hui; Zhao, Dan; He, Li; Chen, Jin; Li, Guocai

    2016-11-01

    Human health has been seriously endangered by highly prevalent salmonellosis and multidrug-resistant Salmonella strains. Current vaccines suffer from variable immune-protective effects, so more effective ones are needed to control Salmonella infection : Bacterial ghosts have been produced by the expression of lysis gene E from bacteriophage PhiX174 and can be filled with considerable exogenous substances such as DNA or drugs as a novel platform. In this study, Salmonella enteritidis (SE) ghosts were developed and loaded with Neisseria gonorrhoeae porin B (porB) to construct a novel inactive vaccine. Our new studies show that SE ghosts loaded with porB displayed increased production of pro-inflammatory cytokines (IL-1β, IL-6, IL-10 and IL-12p70) in bone marrow-derived dendritic cells (BMDCs), and elicited significantly higher specific systemic and mucosal immune responses to Salmonella than SE ghosts alone. In addition, the novel porB-loaded ghosts conferred higher protective effects on virulent Salmonella challenge. For the first time, we demonstrate that N. gonorrhoeae porB, as a novel adjuvant, can increase the immunogenicity of SE ghosts. Our studies suggested that Salmonella enteritidis ghosts loaded with Neisseria gonorrhoeae porin B might be a useful mucosal Salmonella vaccine candidate for practical use in the future. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  9. Colicinogeny in Salmonella serovars isolated in Brazil

    Directory of Open Access Journals (Sweden)

    Leila Carvalho Campos

    1988-06-01

    Full Text Available A study of colicinogeny was made in 748 strains of Salmonella (97 serovars isolated from different sources; human (291, animal (119, environmental (141, food (102 and animal feed (95. Colicin production was detected in 64 strains (8.6%, particularly isolated from foods (30.4%. Col. E1 (53 and Ia (44 were the most frequently observed, especially in S. agona for environment and food sources. Col V production was identified in 5 strains of S. typhimurium within 8 producer cultures isolated from humans. Its relationship with the sources and serovars of Salmonella are discussed.Investigou-se a produção de colicina em 748 amostras de Salmonella (97 sorovares advindas de díferentes fontes: humana (291, animal (119, ambiental (141, de alimentos (102 e rações (95. Detectaram-se 64 amostras (8,6% colicinogênicas, particularmente isoladas de alimentos (30,4%. ColE1 (53 e Ia (44 foram as mais freqüentes, especialmente no sorovar S, agona, de origem ambiental e de alimentos. Identificou-se também a produção de col V em 5 amostras de S. typhimurium dentre 8 culturas produtoras de origem humana. Discute-se a relação entre a capacidade colicinogênica e as fontes e sorovares de Salmonella.

  10. Lack of the PGA exopolysaccharide in Salmonella as an adaptive trait for survival in the host.

    Science.gov (United States)

    Echeverz, Maite; García, Begoña; Sabalza, Amaia; Valle, Jaione; Gabaldón, Toni; Solano, Cristina; Lasa, Iñigo

    2017-05-01

    Many bacteria build biofilm matrices using a conserved exopolysaccharide named PGA or PNAG (poly-β-1,6-N-acetyl-D-glucosamine). Interestingly, while E. coli and other members of the family Enterobacteriaceae encode the pgaABCD operon responsible for PGA synthesis, Salmonella lacks it. The evolutionary force driving this difference remains to be determined. Here, we report that Salmonella lost the pgaABCD operon after the divergence of Salmonella and Citrobacter clades, and previous to the diversification of the currently sequenced Salmonella strains. Reconstitution of the PGA machinery endows Salmonella with the capacity to produce PGA in a cyclic dimeric GMP (c-di-GMP) dependent manner. Outside the host, the PGA polysaccharide does not seem to provide any significant benefit to Salmonella: resistance against chlorine treatment, ultraviolet light irradiation, heavy metal stress and phage infection remained the same as in a strain producing cellulose, the main biofilm exopolysaccharide naturally produced by Salmonella. In contrast, PGA production proved to be deleterious to Salmonella survival inside the host, since it increased susceptibility to bile salts and oxidative stress, and hindered the capacity of S. Enteritidis to survive inside macrophages and to colonize extraintestinal organs, including the gallbladder. Altogether, our observations indicate that PGA is an antivirulence factor whose loss may have been a necessary event during Salmonella speciation to permit survival inside the host.

  11. Fostering diffusion of scientific contents of National Society Cardiovascular Journals: The new ESC search engine.

    Science.gov (United States)

    Alfonso, Fernando; Gonçalves, Lino; Pinto, Fausto; Timmis, Adam; Ector, Hugo; Ambrosio, Giuseppe; Vardas, Panos

    2015-05-01

    European Society of Cardiology (ESC) National Society Cardiovascular Journals (NSCJs) are high-quality biomedical journals focused on cardiovascular diseases. The Editors' Network of the ESC devises editorial initiatives aimed at improving the scientific quality and diffusion of NSCJ. In this article we will discuss on the importance of the Internet, electronic editions and open access strategies on scientific publishing. Finally, we will propose a new editorial initiative based on a novel electronic tool on the ESC web-page that may further help to increase the dissemination of contents and visibility of NSCJs. Copyright © 2013 Sociedade Portuguesa de Cardiologia. Published by Elsevier España. All rights reserved.

  12. Fostering diffusion of scientific contents of National Society Cardiovascular Journals: The new ESC search engine

    Directory of Open Access Journals (Sweden)

    Fernando Alfonso

    2013-12-01

    Full Text Available European Society of Cardiology (ESC National Society Cardiovascular Journals (NSCJs are high-quality biomedical journals focused on cardiovascular diseases. The Editors’ Network of the ESC devises editorial initiatives aimed at improving the scientific quality and diffusion of NSCJ. In this article we will discuss on the importance of the Internet, electronic editions and open access strategies on scientific publishing. Finally, we will propose a new editorial initiative based on a novel electronic tool on the ESC web-page that may further help to increase the dissemination of contents and visibility of NSCJs.

  13. The risk of salmonellae shedding by dogs fed Salmonella-contaminated commercial raw food diets

    OpenAIRE

    Finley, Rita; Ribble, Carl; Aramini, Jeff; Vandermeer, Meredith; Popa, Maria; Litman, Marcus; Reid-Smith, Richard

    2007-01-01

    Twenty-eight research dogs were enrolled to determine the prevalence of salmonellae shedding after consumption of 1 Salmonella-contaminated commercial raw food diet meal. Sixteen dogs were exposed to Salmonella-contaminated commercial raw food diets and 12 to Salmonella-free commercial raw food diets. Seven of the exposed dogs shed salmonellae 1–7 days after consumption of Salmonella-contaminated raw food diets. None of the dogs fed Salmonella-free diets shed salmonellae. No clinical signs we...

  14. Salmonella contamination of cereal ingredients for animal feeds.

    Science.gov (United States)

    Davies, R H; Wales, A D

    2013-10-25

    Cereal ingredients for animal feedstuffs may become contaminated by Salmonella on their farms of origin. This is often concentrated in multiple foci, owing to contamination by rodents and other wildlife which may be missed by routine sampling, and may involve serovars of particular public health significance, such as Salmonella Typhimurium (STM). The study examined such contamination in domestically-produced cereal ingredients in the United Kingdom. Cereal-producing farms with associated cattle or pig enterprises (43) and feedmills (6) were investigated, following the isolation of STM from their premises (feedmills) or STM DT104 from their livestock (farms) by routine surveillance. Cereal samples from feedmills yielded two STM isolates from the same premises, of the same phage types as were isolated from wild bird faeces at ingredient intake and product loading areas. Farm investigations identified numerous Salmonella serovars, including STM, on grain harvesting and handling equipment, in grain storage areas, and in wildlife samples. Mice were removed from one pig farm and shed Salmonella Derby and Salmonella Bovismorbificans for 10 months afterwards. Grain stores more than one kilometre away from livestock areas were rarely found to be contaminated with STM. The principal issues with Salmonella contamination of cereals appeared to be the use of livestock areas as temporary grain stores on cattle farms, and access to stored grain by wildlife and domestic animals. Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.

  15. Salmonella enteritidis and other Salmonella in laying hens and eggs from flocks with Salmonella in their environment.

    Science.gov (United States)

    Poppe, C; Johnson, R P; Forsberg, C M; Irwin, R J

    1992-01-01

    Seven Canadian layer flocks with Salmonella enteritidis in their environment were investigated to determine the numbers of hens infected with S. enteritidis, the localization of S. enteritidis in organs of infected hens and the numbers of S. enteritidis-infected eggs produced by two affected flocks. By a microagglutination test (MAT) using S. pullorum antigens, these flocks had more seropositive hens (mean 51.9 +/- 16.9%) than two Salmonella-free flocks (mean 13.0 +/- 4.2%). Culture of tissues of 580 hens (433 seropositive) from the seven flocks detected 26 (4.5%) S. enteritidis-infected hens from two flocks. In one flock, 2/150 hens were infected with S. enteritidis phage type (PT) 8, which was confined to the ceca, and no Salmonella spp. were isolated from 2520 eggs (one day's lay). In the second flock, where 24/150 hens were infected with S. enteritidis PT13, extraintestinal infection was found in nine hens and involved the ovaries and/or oviduct in two hens. Salmonella enteritidis PT13 was isolated from one sample of egg contents and from one sample of cracked shells from among 14,040 eggs (one day's lay) from this flock. The overall prevalence of S. enteritidis-contaminated eggs from the two flocks with infected hens was less than 0.06%. Other Salmonella spp. isolated were S. heidelberg from 58 hens (10%), and S. hadar, S. mbandaka and S. typhimurium from one hen (0.2%) each. The MAT with antigens of S. pullorum had a sensitivity of 81% and a specificity of 24% for detecting S. enteritidis-infected hens.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:1423059

  16. Antimicrobial resistance among Salmonella enterica serovar Infantis from broiler carcasses in Serbia

    Science.gov (United States)

    Nikolić, A.; Baltić, T.; Velebit, B.; Babić, M.; Milojević, L.; Đorđević, V.

    2017-09-01

    This study aimed to investigate antimicrobial resistance of Salmonella Infantis isolates from poultry carcasses in Serbia. A total of 48 Salmonella isolates were examined for antimicrobial resistance. A panel of 10 antibiotics was selected for testing. Isolates showed resistance to sulfamethoxazole, ceftazidime and cefotaxime (100%). However, the highest number of Salmonella Infantis isolates were sensitive to chloramphenicol. The usage of antibiotics in food producing animals could result in antimicrobial resistance pathogenic bacteria especially Salmonella spp. in poultry, which may be transmitted to humans through the food chain and increase risk of treatment failures.

  17. European Society of Cardiology (ESC) Annual Congress Report From Barcelona 2017.

    Science.gov (United States)

    Satoh, Kimio; Takahashi, Jun; Matsumoto, Yasuharu; Tatebe, Shunsuke; Aoki, Tatsuo; Kikuchi, Yoku; Hao, Kiyotaka; Ohyama, Kazuma; Nogi, Masamichi; Suda, Akira; Kasahara, Shintaro; Sato, Koichi; Ichijo, Sadamitsu; Shimokawa, Hiroaki

    2017-11-02

    From August 26th to 30th, the 2017 Annual Congress of the European Society of Cardiology (ESC 2017) was held in Barcelona, Spain. Despite the terrorism tradegy just before the ESC congress, the congress attracted many medical professionals from all over the world to discuss the recent topics in cardiovascular medicine in more than 500 sessions, including COMPASS (Cardiovascular OutcoMes for People using Anticoagulation StrategieS Trial), CANTOS (Canakinumab Anti-Inflammatory Thrombosis Outcomes Study), and ORION (which assessed the effect of a novel siRNA inhibitor to PCSK9 on reductions in low-density lipoprotein cholesterol). Japanese cardiologists and the Japanese Circulation Society greatly contributed to the congress. This report briefly introduces some late-breaking registry results, late-breaking clinical trials, and ESC Guidelines from the ESC 2017 Congress.

  18. ROSETTA-ORBITER 67P RPCIES 2 ESC2 V1.0

    Data.gov (United States)

    National Aeronautics and Space Administration — This dataset contains EDITED RAW DATA of the Rosetta RPCIES instrument taken during the comet escort 2 phase (ESC2). The target of this phase was comet...

  19. Salmonella from Baby Turtles

    Centers for Disease Control (CDC) Podcasts

    2017-01-09

    Dr. Stacey Bosch, a veterinarian with CDC, discusses her article on Salmonella infections associated with baby turtles.  Created: 1/9/2017 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 1/9/2017.

  20. Salmonella Infections - Multiple Languages

    Science.gov (United States)

    ... to Know - 한국어 (Korean) PDF Centers for Disease Control and Prevention Spanish (español) Expand Section Salmonella Infections: MedlinePlus Health Topic - English Infecciones por salmonela: Tema de salud de MedlinePlus - español ( ...