EPA's Radioactive Source Program

International Nuclear Information System (INIS)

Kopsick, D.

2004-01-01

The US EPA is the lead Federal agency for emergency responses to unknown radiological materials, not licensed, owned or operated by a Federal agency or an Agreement state (Federal Radiological Emergency Response Plan, 1996). The purpose of EPA's clean materials programme is to keep unwanted and unregulated radioactive material out of the public domain. This is achieved by finding and securing lost sources, maintaining control of existing sources and preventing future losses. The focus is on both, domestic and international fronts. The domestic program concentrates on securing lost sources, preventing future losses, alternative technologies like tagging of radioactive sources in commerce, pilot radioactive source roundup, training programs, scrap metal and metal processing facilities, the demolition industry, product stewardship and alternatives to radioactive devices (fewer radioactive source devices means fewer orphan sources). The international program consists of securing lost sources, preventing future losses, radiation monitoring of scrap metal at ports and the international scrap metal monitoring protocol

Lead Sampling Protocols: Why So Many and What Do They Tell You?

Science.gov (United States)

Sampling protocols can be broadly categorized based on their intended purpose of 1) Pb regulatory compliance/corrosion control efficacy, 2) Pb plumbing source determination or Pb type identification, and 3) Pb exposure assessment. Choosing the appropriate protocol is crucial to p...

A pilot effectiveness study of the Enhancing Parenting Skills (EPaS) 2014 programme for parents of children with behaviour problems: study protocol for a randomised controlled trial

OpenAIRE

Williams, Margiad Elen; Hutchings, Judy

2015-01-01

Background The Enhancing Parenting Skills (EPaS) 2014 programme is a home-based, health visitor-delivered parenting support programme for parents of children with identified behaviour problems. This trial aims to evaluate the effectiveness of the EPaS 2014 programme compared to a waiting-list treatment as usual control group. Methods/Design This is a pragmatic, multicentre randomised controlled trial. Sixty health visitors will each be asked to identify two families that have a child scoring ...

Surface and subsurface cleanup protocol for radionuclides, Gunnison, Colorado, UMTRA project processing site. Final [report

Energy Technology Data Exchange (ETDEWEB)

1993-09-01

Surface and subsurface soil cleanup protocols for the Gunnison, Colorado, processing sits are summarized as follows: In accordance with EPA-promulgated land cleanup standards (40 CFR 192), in situ Ra-226 is to be cleaned up based on bulk concentrations not exceeding 5 and 15 pCi/g in 15-cm surface and subsurface depth increments, averaged over 100-m{sup 2} grid blocks, where the parent Ra-226 concentrations are greater than, or in secular equilibrium with, the Th-230 parent. A bulk interpretation of these EPA standards has been accepted by the Nuclear Regulatory Commission (NRC), and while the concentration of the finer-sized soil fraction less than a No. 4 mesh sieve contains the higher concentration of radioactivity, the bulk approach in effect integrates the total sample radioactivity over the entire sample mass. In locations where Th-230 has differentially migrated in subsoil relative to Ra-226, a Th-230 cleanup protocol has been developed in accordance with Supplemental Standard provisions of 40 CFR 192 for NRC/Colorado Department of Health (CDH) approval for timely implementation. Detailed elements of the protocol are contained in Appendix A, Generic Protocol from Thorium-230 Cleanup/Verification at UMTRA Project Processing Sites. The cleanup of other radionuclides or nonradiological hazards that pose a significant threat to the public and the environment will be determined and implemented in accordance with pathway analysis to assess impacts and the implications of ALARA specified in 40 CFR 192 relative to supplemental standards.

The Gas Sampling Interval Effect on V˙O2peak Is Independent of Exercise Protocol.

Science.gov (United States)

Scheadler, Cory M; Garver, Matthew J; Hanson, Nicholas J

2017-09-01

There is a plethora of gas sampling intervals available during cardiopulmonary exercise testing to measure peak oxygen consumption (V˙O2peak). Different intervals can lead to altered V˙O2peak. Whether differences are affected by the exercise protocol or subject sample is not clear. The purpose of this investigation was to determine whether V˙O2peak differed because of the manipulation of sampling intervals and whether differences were independent of the protocol and subject sample. The first subject sample (24 ± 3 yr; V˙O2peak via 15-breath moving averages: 56.2 ± 6.8 mL·kg·min) completed the Bruce and the self-paced V˙O2max protocols. The second subject sample (21.9 ± 2.7 yr; V˙O2peak via 15-breath moving averages: 54.2 ± 8.0 mL·kg·min) completed the Bruce and the modified Astrand protocols. V˙O2peak was identified using five sampling intervals: 15-s block averages, 30-s block averages, 15-breath block averages, 15-breath moving averages, and 30-s block averages aligned to the end of exercise. Differences in V˙O2peak between intervals were determined using repeated-measures ANOVAs. The influence of subject sample on the sampling effect was determined using independent t-tests. There was a significant main effect of sampling interval on V˙O2peak (first sample Bruce and self-paced V˙O2max P sample Bruce and modified Astrand P sampling intervals followed a similar pattern for each protocol and subject sample, with 15-breath moving average presenting the highest V˙O2peak. The effect of manipulating gas sampling intervals on V˙O2peak appears to be protocol and sample independent. These findings highlight our recommendation that the clinical and scientific community request and report the sampling interval whenever metabolic data are presented. The standardization of reporting would assist in the comparison of V˙O2peak.

A modified FASP protocol for high-throughput preparation of protein samples for mass spectrometry.

Directory of Open Access Journals (Sweden)

Jeremy Potriquet

Full Text Available To facilitate high-throughput proteomic analyses we have developed a modified FASP protocol which improves the rate at which protein samples can be processed prior to mass spectrometry. Adapting the original FASP protocol to a 96-well format necessitates extended spin times for buffer exchange due to the low centrifugation speeds tolerated by these devices. However, by using 96-well plates with a more robust polyethersulfone molecular weight cutoff membrane, instead of the cellulose membranes typically used in these devices, we could use isopropanol as a wetting agent, decreasing spin times required for buffer exchange from an hour to 30 minutes. In a typical work flow used in our laboratory this equates to a reduction of 3 hours per plate, providing processing times similar to FASP for the processing of up to 96 samples per plate. To test whether our modified protocol produced similar results to FASP and other FASP-like protocols we compared the performance of our modified protocol to the original FASP and the more recently described eFASP and MStern-blot. We show that all FASP-like methods, including our modified protocol, display similar performance in terms of proteins identified and reproducibility. Our results show that our modified FASP protocol is an efficient method for the high-throughput processing of protein samples for mass spectral analysis.

A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples

Directory of Open Access Journals (Sweden)

Shivani Gupta

2017-01-01

Full Text Available The prevalence of drug resistance (DR mutations in people with HIV-1 infection, particularly those with low-level viremia (LLV, supports the need to improve the sensitivity of amplification methods for HIV DR genotyping in order to optimize antiretroviral regimen and facilitate HIV-1 DR surveillance and relevant research. Here we report on a fully validated PCR-based protocol that achieves consistent amplification of the protease (PR and reverse transcriptase (RT regions of HIV-1 pol gene across many HIV-1 subtypes from LLV plasma samples. HIV-spiked plasma samples from the External Quality Assurance Program Oversight Laboratory (EQAPOL, covering various HIV-1 subtypes, as well as clinical specimens were used to optimize and validate the protocol. Our results demonstrate that this protocol has a broad HIV-1 subtype coverage and viral load span with high sensitivity and reproducibility. Moreover, the protocol is robust even when plasma sample volumes are limited, the HIV viral load is unknown, and/or the HIV subtype is undetermined. Thus, the protocol is applicable for the initial amplification of the HIV-1 PR and RT genes required for subsequent genotypic DR assays.

A simplified field protocol for genetic sampling of birds using buccal swabs

Science.gov (United States)

Vilstrup, Julia T.; Mullins, Thomas D.; Miller, Mark P.; McDearman, Will; Walters, Jeffrey R.; Haig, Susan M.

2018-01-01

DNA sampling is an essential prerequisite for conducting population genetic studies. For many years, blood sampling has been the preferred method for obtaining DNA in birds because of their nucleated red blood cells. Nonetheless, use of buccal swabs has been gaining favor because they are less invasive yet still yield adequate amounts of DNA for amplifying mitochondrial and nuclear markers; however, buccal swab protocols often include steps (e.g., extended air-drying and storage under frozen conditions) not easily adapted to field settings. Furthermore, commercial extraction kits and swabs for buccal sampling can be expensive for large population studies. We therefore developed an efficient, cost-effective, and field-friendly protocol for sampling wild birds after comparing DNA yield among 3 inexpensive buccal swab types (2 with foam tips and 1 with a cotton tip). Extraction and amplification success was high (100% and 97.2% respectively) using inexpensive generic swabs. We found foam-tipped swabs provided higher DNA yields than cotton-tipped swabs. We further determined that omitting a drying step and storing swabs in Longmire buffer increased efficiency in the field while still yielding sufficient amounts of DNA for detailed population genetic studies using mitochondrial and nuclear markers. This new field protocol allows time- and cost-effective DNA sampling of juveniles or small-bodied birds for which drawing blood may cause excessive stress to birds and technicians alike.

Discrepancies in sample size calculations and data analyses reported in randomised trials: comparison of publications with protocols

DEFF Research Database (Denmark)

Chan, A.W.; Hrobjartsson, A.; Jorgensen, K.J.

2008-01-01

OBJECTIVE: To evaluate how often sample size calculations and methods of statistical analysis are pre-specified or changed in randomised trials. DESIGN: Retrospective cohort study. Data source Protocols and journal publications of published randomised parallel group trials initially approved...... in 1994-5 by the scientific-ethics committees for Copenhagen and Frederiksberg, Denmark (n=70). MAIN OUTCOME MEASURE: Proportion of protocols and publications that did not provide key information about sample size calculations and statistical methods; proportion of trials with discrepancies between...... of handling missing data was described in 16 protocols and 49 publications. 39/49 protocols and 42/43 publications reported the statistical test used to analyse primary outcome measures. Unacknowledged discrepancies between protocols and publications were found for sample size calculations (18/34 trials...

Optical code-division multiple-access protocol with selective retransmission

Science.gov (United States)

Mohamed, Mohamed A. A.; Shalaby, Hossam M. H.; El-Badawy, El-Sayed A.

2006-05-01

An optical code-division multiple-access (OCDMA) protocol based on selective retransmission technique is proposed. The protocol is modeled using a detailed state diagram and is analyzed using equilibrium point analysis (EPA). Both traditional throughput and average delay are used to examine its performance for several network parameters. In addition, the performance of the proposed protocol is compared to that of the R3T protocol, which is based on a go-back-n technique. Our results show that a higher performance is achieved by the proposed protocol at the expense of system complexity.

76 FR 9780 - Notification of Deletion of System of Records; EPA Parking Control Office File (EPA-10) and EPA...

Science.gov (United States)

2011-02-22

... System of Records; EPA Parking Control Office File (EPA-10) and EPA Transit and Guaranteed Ride Home... Environmental Protection Agency (EPA) is deleting the systems of records for EPA Parking Control Office File... through the EPA Internet under the ``Federal Register'' listings at http://www.epa.gov/fedrgstr/ . Dated...

National Coastal Condition Assessment (NCCA) Sampling Areas Map, Hawaiian Islands Shoreline, 2015, US EPA Region 9

Data.gov (United States)

U.S. Environmental Protection Agency — The National Coastal Condition Assessment (NCCA) is a national coastal monitoring program with rigorous quality assurance protocols and standardized sampling...

GAC-EPA

CERN Multimedia

GAC-EPA

2012-01-01

It saddens us deeply to learn of the passing away of Jean-Paul Diss who died suddenly on 7 June 2012 at his home.  A tribute can be read on the GAC-EPA site. * * * * * Information: http://gac-epa.org/ e-mail: gac-epa@gac-epa.org

A pilot effectiveness study of the Enhancing Parenting Skills (EPaS) 2014 programme for parents of children with behaviour problems: study protocol for a randomised controlled trial.

Science.gov (United States)

Williams, Margiad Elen; Hutchings, Judy

2015-05-20

The Enhancing Parenting Skills (EPaS) 2014 programme is a home-based, health visitor-delivered parenting support programme for parents of children with identified behaviour problems. This trial aims to evaluate the effectiveness of the EPaS 2014 programme compared to a waiting-list treatment as usual control group. This is a pragmatic, multicentre randomised controlled trial. Sixty health visitors will each be asked to identify two families that have a child scoring above the clinical cut-off for behaviour problems using the Eyberg Child Behaviour Inventory (ECBI). Families recruited to the trial will be randomised in a 1:1 ratio into an intervention or waiting-list control group. Randomisation will occur within health visitor to ensure that each health visitor has one intervention family and one control family. The primary outcome is change in child behaviour problems as measured by the parent-reported ECBI. Secondary outcomes include other measures of child behaviour, parent behaviour, and parental depression as measured by parent-reports and an independent observation of parent and child behaviour. Follow-up measures will be collected 6-months after the collection of baseline measures. This is the first rigorous evaluation of the EPaS 2014 programme. The trial will provide important information on the effectiveness of a one-to-one home-based intervention, delivered by health visitors, for pre-school children with behaviour problems. It will also examine potential mediating (improved parent behaviour and/or improved parental depression) and moderating (single parent, teenage parent, poverty, low education level) factors. Current Controlled Trials ISRCTN06867279 (18 June 2014).

Pilot studies for the North American Soil Geochemical Landscapes Project - Site selection, sampling protocols, analytical methods, and quality control protocols

Science.gov (United States)

Smith, D.B.; Woodruff, L.G.; O'Leary, R. M.; Cannon, W.F.; Garrett, R.G.; Kilburn, J.E.; Goldhaber, M.B.

2009-01-01

In 2004, the US Geological Survey (USGS) and the Geological Survey of Canada sampled and chemically analyzed soils along two transects across Canada and the USA in preparation for a planned soil geochemical survey of North America. This effort was a pilot study to test and refine sampling protocols, analytical methods, quality control protocols, and field logistics for the continental survey. A total of 220 sample sites were selected at approximately 40-km intervals along the two transects. The ideal sampling protocol at each site called for a sample from a depth of 0-5 cm and a composite of each of the O, A, and C horizons. The Ca, Fe, K, Mg, Na, S, Ti, Ag, As, Ba, Be, Bi, Cd, Ce, Co, Cr, Cs, Cu, Ga, In, La, Li, Mn, Mo, Nb, Ni, P, Pb, Rb, Sb, Sc, Sn, Sr, Te, Th, Tl, U, V, W, Y, and Zn by inductively coupled plasma-mass spectrometry and inductively coupled plasma-atomic emission spectrometry following a near-total digestion in a mixture of HCl, HNO3, HClO4, and HF. Separate methods were used for Hg, Se, total C, and carbonate-C on this same size fraction. Only Ag, In, and Te had a large percentage of concentrations below the detection limit. Quality control (QC) of the analyses was monitored at three levels: the laboratory performing the analysis, the USGS QC officer, and the principal investigator for the study. This level of review resulted in an average of one QC sample for every 20 field samples, which proved to be minimally adequate for such a large-scale survey. Additional QC samples should be added to monitor within-batch quality to the extent that no more than 10 samples are analyzed between a QC sample. Only Cr (77%), Y (82%), and Sb (80%) fell outside the acceptable limits of accuracy (% recovery between 85 and 115%) because of likely residence in mineral phases resistant to the acid digestion. A separate sample of 0-5-cm material was collected at each site for determination of organic compounds. A subset of 73 of these samples was analyzed for a suite of

Robowell: An automated process for monitoring ground water quality using established sampling protocols

Science.gov (United States)

Granato, G.E.; Smith, K.P.

1999-01-01

Robowell is an automated process for monitoring selected ground water quality properties and constituents by pumping a well or multilevel sampler. Robowell was developed and tested to provide a cost-effective monitoring system that meets protocols expected for manual sampling. The process uses commercially available electronics, instrumentation, and hardware, so it can be configured to monitor ground water quality using the equipment, purge protocol, and monitoring well design most appropriate for the monitoring site and the contaminants of interest. A Robowell prototype was installed on a sewage treatment plant infiltration bed that overlies a well-studied unconfined sand and gravel aquifer at the Massachusetts Military Reservation, Cape Cod, Massachusetts, during a time when two distinct plumes of constituents were released. The prototype was operated from May 10 to November 13, 1996, and quality-assurance/quality-control measurements demonstrated that the data obtained by the automated method was equivalent to data obtained by manual sampling methods using the same sampling protocols. Water level, specific conductance, pH, water temperature, dissolved oxygen, and dissolved ammonium were monitored by the prototype as the wells were purged according to U.S Geological Survey (USGS) ground water sampling protocols. Remote access to the data record, via phone modem communications, indicated the arrival of each plume over a few days and the subsequent geochemical reactions over the following weeks. Real-time availability of the monitoring record provided the information needed to initiate manual sampling efforts in response to changes in measured ground water quality, which proved the method and characterized the screened portion of the plume in detail through time. The methods and the case study described are presented to document the process for future use.

Reducing the sampling periods required in protocols for establishing ammonia emissions from pig fattening buildings using measurements and modelling

NARCIS (Netherlands)

Mosquera Losada, J.; Ogink, N.W.M.

2011-01-01

Ammonia (NH(3)) emission factors for animal housing systems in the Netherlands are based on measurements using standardised measurement protocols. Both the original Green Label (GL) protocol and the newly developed multi-site sampling protocol are based on year-round sampling periods. The objective

A rapid and efficient DNA extraction protocol from fresh and frozen human blood samples.

Science.gov (United States)

Guha, Pokhraj; Das, Avishek; Dutta, Somit; Chaudhuri, Tapas Kumar

2018-01-01

Different methods available for extraction of human genomic DNA suffer from one or more drawbacks including low yield, compromised quality, cost, time consumption, use of toxic organic solvents, and many more. Herein, we aimed to develop a method to extract DNA from 500 μL of fresh or frozen human blood. Five hundred microliters of fresh and frozen human blood samples were used for standardization of the extraction procedure. Absorbance at 260 and 280 nm, respectively, (A 260 /A 280 ) were estimated to check the quality and quantity of the extracted DNA sample. Qualitative assessment of the extracted DNA was checked by Polymerase Chain reaction and double digestion of the DNA sample. Our protocol resulted in average yield of 22±2.97 μg and 20.5±3.97 μg from 500 μL of fresh and frozen blood, respectively, which were comparable to many reference protocols and kits. Besides yielding bulk amount of DNA, our protocol is rapid, economical, and avoids toxic organic solvents such as Phenol. Due to unaffected quality, the DNA is suitable for downstream applications. The protocol may also be useful for pursuing basic molecular researches in laboratories having limited funds. © 2017 Wiley Periodicals, Inc.

Development of a protocol for sampling and analysis of ballast water in Jamaica

Directory of Open Access Journals (Sweden)

Achsah A Mitchell

2014-09-01

Full Text Available The transfer of ballast by the international shipping industry has negatively impacted the environment. To design such a protocol for the area, the ballast water tanks of seven bulk cargo vessels entering a Jamaican port were sampled between January 28, 2010 and August 17, 2010. Vessels originated from five ports and used three main routes, some of which conducted ballast water exchange. Twenty-six preserved and 22 live replicate zooplankton samples were obtained. Abundance and richness were higher than at temperate ports. Exchange did not alter the biotic composition but reduced the abundance. Two of the live sample replicates, containing 31.67 and 16.75 viable individuals m-3, were non-compliant with the International Convention for the Control and Management of Ships’ Ballast Water and Sediments. Approximately 12% of the species identified in the ballast water were present in the waters nearest the port in 1995 and 11% were present in the entire bay in 2005. The protocol designed from this study can be used to aid the establishment of a ballast water management system in the Caribbean or used as a foundation for the development of further protocols.

Evaluation of sample preparation protocols for spider venom profiling by MALDI-TOF MS.

Science.gov (United States)

Bočánek, Ondřej; Šedo, Ondrej; Pekár, Stano; Zdráhal, Zbyněk

2017-07-01

Spider venoms are highly complex mixtures containing biologically active substances with potential for use in biotechnology or pharmacology. Fingerprinting of venoms by Matrix-Assisted Laser Desorption-Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) is a thriving technology, enabling the rapid detection of peptide/protein components that can provide comparative information. In this study, we evaluated the effects of sample preparation procedures on MALDI-TOF mass spectral quality to establish a protocol providing the most reliable analytical outputs. We adopted initial sample preparation conditions from studies already published in this field. Three different MALDI matrixes, three matrix solvents, two sample deposition methods, and different acid concentrations were tested. As a model sample, venom from Brachypelma albopilosa was used. The mass spectra were evaluated on the basis of absolute and relative signal intensities, and signal resolution. By conducting three series of analyses at three weekly intervals, the reproducibility of the mass spectra were assessed as a crucial factor in the selection for optimum conditions. A sample preparation protocol based on the use of an HCCA matrix dissolved in 50% acetonitrile with 2.5% TFA deposited onto the target by the dried-droplet method was found to provide the best results in terms of information yield and repeatability. We propose that this protocol should be followed as a standard procedure, enabling the comparative assessment of MALDI-TOF MS spider venom fingerprints. Copyright © 2017 Elsevier Ltd. All rights reserved.

Coping with EPA's new petroleum industry storm water permits

International Nuclear Information System (INIS)

Veal, S.C.; Whitescarver, J.P.

1994-01-01

The United States Environmental Protection Agency has just released for public comment its so-called multi-sector industry specific storm water permit. This permit -- developed in response to the 730 group storm water permit applications submitted in 1992 to EPA -- proposes the establishment of specific runoff sampling and facility design requirements for at least two petroleum industry sectors. This proposed permit establishes specific conditions for the oil and gas extraction section (SIC group 13) and for lubricant manufacturers (SIC 2992). Permit conditions are also established for allied industrial sectors such as the chemical, transportation and asphalt materials industries. By most standards, the proposed permit is much tougher than EPA's baseline general permit for storm water discharges which was released in September of 1992. For example, under the proposal, most industries are required to perform periodic storm water sampling. EPA has also established storm water effluent and performance standards for several industrial categories. This paper will discuss the petroleum industry specific conditions of the new permit. The paper will also discuss the results of the industry-wide storm water sampling efforts undertaken by more than 300 oil patch facilities across the country. In particular, sampling results will be discussed in the context to the permit conditions proposed by EPA. The paper will also discuss strategies for dealing with the new permits

Optimization of a sample processing protocol for recovery of Bacillus anthracis spores from soil

Science.gov (United States)

Silvestri, Erin E.; Feldhake, David; Griffin, Dale; Lisle, John T.; Nichols, Tonya L.; Shah, Sanjiv; Pemberton, A; Schaefer III, Frank W

2016-01-01

Following a release of Bacillus anthracis spores into the environment, there is a potential for lasting environmental contamination in soils. There is a need for detection protocols for B. anthracis in environmental matrices. However, identification of B. anthracis within a soil is a difficult task. Processing soil samples helps to remove debris, chemical components, and biological impurities that can interfere with microbiological detection. This study aimed to optimize a previously used indirect processing protocol, which included a series of washing and centrifugation steps. Optimization of the protocol included: identifying an ideal extraction diluent, variation in the number of wash steps, variation in the initial centrifugation speed, sonication and shaking mechanisms. The optimized protocol was demonstrated at two laboratories in order to evaluate the recovery of spores from loamy and sandy soils. The new protocol demonstrated an improved limit of detection for loamy and sandy soils over the non-optimized protocol with an approximate matrix limit of detection at 14 spores/g of soil. There were no significant differences overall between the two laboratories for either soil type, suggesting that the processing protocol will be robust enough to use at multiple laboratories while achieving comparable recoveries.

Minimal sampling protocol for accurate estimation of urea production: a study with oral [13C]urea in fed and fasted piglets

NARCIS (Netherlands)

Oosterveld, Michiel J. S.; Gemke, Reinoud J. B. J.; Dainty, Jack R.; Kulik, Willem; Jakobs, Cornelis; de Meer, Kees

2005-01-01

An oral [13C]urea protocol may provide a simple method for measurement of urea production. The validity of single pool calculations in relation to a reduced sampling protocol was assessed. In eight fed and five fasted piglets, plasma urea enrichments from a 10 h sampling protocol were measured

GAC-EPA

CERN Multimedia

GAC-EPA

2014-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 4 février de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org. * * * * * Carte de membre de l'Association du personnel du CERN Les membres GAC-EPA qui souhaitent recevoir une carte de membre AP en 2014 doivent  en faire la demande par email à secretariat@gac-epa.org, ou par lettre au secrétaire ...

Zoonoses action plan Salmonella monitoring programme: an investigation of the sampling protocol.

Science.gov (United States)

Snary, E L; Munday, D K; Arnold, M E; Cook, A J C

2010-03-01

The Zoonoses Action Plan (ZAP) Salmonella Programme was established by the British Pig Executive to monitor Salmonella prevalence in quality-assured British pigs at slaughter by testing a sample of pigs with a meat juice enzyme-linked immunosorbent assay for antibodies against group B and C(1) Salmonella. Farms were assigned a ZAP level (1 to 3) depending on the monitored prevalence, and ZAP 2 or 3 farms were required to act to reduce the prevalence. The ultimate goal was to reduce the risk of human salmonellosis attributable to British pork. A mathematical model has been developed to describe the ZAP sampling protocol. Results show that the probability of assigning a farm the correct ZAP level was high, except for farms that had a seroprevalence close to the cutoff points between different ZAP levels. Sensitivity analyses identified that the probability of assigning a farm to the correct ZAP level was dependent on the sensitivity and specificity of the test, the number of batches taken to slaughter each quarter, and the number of samples taken per batch. The variability of the predicted seroprevalence was reduced as the number of batches or samples increased and, away from the cutoff points, the probability of being assigned the correct ZAP level increased as the number of batches or samples increased. In summary, the model described here provided invaluable insight into the ZAP sampling protocol. Further work is required to understand the impact of the program for Salmonella infection in British pig farms and therefore on human health.

Data validation report for the 100-HR-3 Operable Unit first quarter 1994 groundwater sampling data

Energy Technology Data Exchange (ETDEWEB)

Biggerstaff, R.L.

1994-06-24

Westinghouse-Hanford has requested that a minimum of 20% of the total number of Sample Delivery Groups be validated for the 100-HR-3 Operable Unit First Quarter 1994 Groundwater Sampling Investigation. Therefore, the data from the chemical analysis of twenty-four samples from this sampling event and their related quality assurance samples were reviewed and validated to verify that reported sample results were of sufficient quality to support decisions regarding remedial actions performed at this site. The samples were analyzed by Thermo-Analytic Laboratories (TMA) and Roy F. Weston Laboratories (WESTON) using US Environmental Protection Agency (EPA) CLP protocols. Sample analyses included: inorganics; and general chemical parameters. Forty-two samples were validated for radiochemical parameters by TMA and Teledyne.

Data validation report for the 100-HR-3 Operable Unit first quarter 1994 groundwater sampling data

International Nuclear Information System (INIS)

Biggerstaff, R.L.

1994-01-01

Westinghouse-Hanford has requested that a minimum of 20% of the total number of Sample Delivery Groups be validated for the 100-HR-3 Operable Unit First Quarter 1994 Groundwater Sampling Investigation. Therefore, the data from the chemical analysis of twenty-four samples from this sampling event and their related quality assurance samples were reviewed and validated to verify that reported sample results were of sufficient quality to support decisions regarding remedial actions performed at this site. The samples were analyzed by Thermo-Analytic Laboratories (TMA) and Roy F. Weston Laboratories (WESTON) using US Environmental Protection Agency (EPA) CLP protocols. Sample analyses included: inorganics; and general chemical parameters. Forty-two samples were validated for radiochemical parameters by TMA and Teledyne

"Slicer" for EPA

CERN Multimedia

CERN PhotoLab

1983-01-01

During the design of the Electron-Positron-Accumulator (EPA), there was an apprehension about the stability-limit of positron bunch-intensity in the SPS. In case that EPA would be able to produce bunches with intensities exceeding what the SPS could digest, an electrostatic septum was to slice up the EPA beam over 2 or 4 turns, thus lowering the bunch intensity while maintaining fast filling of LEP. The "slicer" septum was built and installed, but thanks to the good appetite of the SPS its use never became necessary. The slicer was removed from EPA to lower the machine impedance.

Protocols for the analytical characterization of therapeutic monoclonal antibodies. II - Enzymatic and chemical sample preparation.

Science.gov (United States)

Bobaly, Balazs; D'Atri, Valentina; Goyon, Alexandre; Colas, Olivier; Beck, Alain; Fekete, Szabolcs; Guillarme, Davy

2017-08-15

The analytical characterization of therapeutic monoclonal antibodies and related proteins usually incorporates various sample preparation methodologies. Indeed, quantitative and qualitative information can be enhanced by simplifying the sample, thanks to the removal of sources of heterogeneity (e.g. N-glycans) and/or by decreasing the molecular size of the tested protein by enzymatic or chemical fragmentation. These approaches make the sample more suitable for chromatographic and mass spectrometric analysis. Structural elucidation and quality control (QC) analysis of biopharmaceutics are usually performed at intact, subunit and peptide levels. In this paper, general sample preparation approaches used to attain peptide, subunit and glycan level analysis are overviewed. Protocols are described to perform tryptic proteolysis, IdeS and papain digestion, reduction as well as deglycosylation by PNGase F and EndoS2 enzymes. Both historical and modern sample preparation methods were compared and evaluated using rituximab and trastuzumab, two reference therapeutic mAb products approved by Food and Drug Administration (FDA) and European Medicines Agency (EMA). The described protocols may help analysts to develop sample preparation methods in the field of therapeutic protein analysis. Copyright © 2017 Elsevier B.V. All rights reserved.

EPA's radon study results

International Nuclear Information System (INIS)

Dowd, R.M.

1988-01-01

Last winter, in cooperation with agencies in 10 states and two metropolitan area counties, EPA measured the indoor air radon concentrations of 14,000 houses, some chosen statistically at random and some by request of the homeowner. Passive measurement methodologies were used, such as exposing a charcoal canister to the air for a few days and allowing the air to migrate in to the charcoal naturally. To reduce dilution of radon by the outside air, the protocol required that the house be shut up; therefore, the study was conducted during winter. The measuring device was placed in the lowest livable area (usually the basement) of each house to maximize potential concentration. It should be noted that these procedures are generally considered to be screening tests because they result in a worst-case measurement rather than a best value. The results of these findings are presented

Protocol for sampling and analysis of bone specimens

International Nuclear Information System (INIS)

Aras, N.K.

2000-01-01

The iliac crest of hip bone was chosen as the most suitable sampling site for several reasons: Local variation in the elemental concentration along the iliac crest is minimal; Iliac crest biopsies are commonly taken clinically on patients; The cortical part of the sample is small (∼2 mm) and can be separated easily from the trabecular bone; The use of the trabecular part of the iliac crest for trace element analysis has the advantage of reflecting rapidly changes in the composition of bone due to external parameters, including medication. Biopsy studies, although in some ways more difficult than autopsy studies, because of the need to obtain the informed consents of the subjects, are potentially more useful than autopsy studies. Thereby many problems of postmortem migration of elements can be avoided and reliable dietary and other data can be collected simultaneously. Select the subjects among the patients undergoing orthopedic surgery due to any reason other than osteoporosis. Follow an established protocol to obtain bone biopsies. Patients undergoing synergy should fill in the 'Osteoporosis Project Questionnaire Form' including information on lifestyle variables, dietary intakes, the reason for surgery etc. If possible, measure the bone mineral density (BMD) prior to removal of the biopsy sample. However it may not possible to have BMD results on all the subjects because of difficulty of DEXA measurement after an accident

GAC-EPA

CERN Multimedia

GAC-EPA

2016-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : le mardi 29 novembre de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

GAC-EPA

CERN Multimedia

GAC-EPA

2013-01-01

Carte de membre de l'Association du personnel du CERN Comme cela a été précisé dans le bulletin d'automne n° 43, les membres GAC-EPA qui souhaitent recevoir une carte de membre AP en 2013 devront en faire la demande, avant le 31 janvier, par email à secretariat@gac-epa.org, ou par lettre au secrétaire du GAC-EPA, p/a Association du personnel CERN, CH-1211 GENEVE 23. Il n'y a pas de tacite reconduction de ces cartes et par conséquent une demande doit être faite chaque année par l'intéressé(e).

The influence of nozzle type, operating pressure, and tank-mixture components on droplet characteristics and the EPA's drift reduction rating

Science.gov (United States)

The introduction of drift reduction technology (DRT) guidelines by the U. S. Environmental Protection Agency (EPA) has established testing protocols for nozzles, agrochemicals, application parameters, and combinations thereof for applying agrochemicals by certified individuals in the United States....

GAC-EPA

CERN Multimedia

GAC-EPA

2015-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 1er décembre de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

Comparison of Different Sample Preparation Protocols Reveals Lysis Buffer-Specific Extraction Biases in Gram-Negative Bacteria and Human Cells.

Science.gov (United States)

Glatter, Timo; Ahrné, Erik; Schmidt, Alexander

2015-11-06

We evaluated different in-solution and FASP-based sample preparation strategies for absolute protein quantification. Label-free quantification (LFQ) was employed to compare different sample preparation strategies in the bacterium Pseudomonas aeruginosa and human embryonic kidney cells (HEK), and organismal-specific differences in general performance and enrichment of specific protein classes were noted. The original FASP protocol globally enriched for most proteins in the bacterial sample, whereas the sodium deoxycholate in-solution strategy was more efficient with HEK cells. Although detergents were found to be highly suited for global proteome analysis, higher intensities were obtained for high-abundant nucleic acid-associated protein complexes, like the ribosome and histone proteins, using guanidine hydrochloride. Importantly, we show for the first time that the observable total proteome mass of a sample strongly depends on the sample preparation protocol, with some protocols resulting in a significant underestimation of protein mass due to incomplete protein extraction of biased protein groups. Furthermore, we demonstrate that some of the observed abundance biases can be overcome by incorporating a nuclease treatment step or, alternatively, a correction factor for complementary sample preparation approaches.

EPA eXcats

Data.gov (United States)

U.S. Environmental Protection Agency — The EPA eXcats is an enterprise-level data tracking application that provides management complaint tracking information for the EPA's Office of Civil Rights (OCR)...

EPA Web Taxonomy

Data.gov (United States)

U.S. Environmental Protection Agency — EPA's Web Taxonomy is a faceted hierarchical vocabulary used to tag web pages with terms from a controlled vocabulary. Tagging enables search and discovery of EPA's...

Easy, fast and environmental friendly method for the simultaneous extraction of the 16 EPA PAHs using magnetic molecular imprinted polymers (mag-MIPs).

Science.gov (United States)

Villar-Navarro, Mercedes; Martín-Valero, María Jesús; Fernández-Torres, Rut Maria; Callejón-Mochón, Manuel; Bello-López, Miguel Ángel

2017-02-15

An easy and environmental friendly method, based on the use of magnetic molecular imprinted polymers (mag-MIPs) is proposed for the simultaneous extraction of the 16 U.S. EPA polycyclic aromatic hydrocarbons (PAHs) priority pollutants. The mag-MIPs based extraction protocol is simple, more sensitive and low organic solvent consuming compared to official methods and also adequate for those PAHs more retained in the particulate matter. The new proposed extraction method followed by HPLC determination has been validated and applied to different types of water samples: tap water, river water, lake water and mineral water. Copyright © 2017 Elsevier B.V. All rights reserved.

MPLEx: a Robust and Universal Protocol for Single-Sample Integrative Proteomic, Metabolomic, and Lipidomic Analyses

Energy Technology Data Exchange (ETDEWEB)

Nakayasu, Ernesto S.; Nicora, Carrie D.; Sims, Amy C.; Burnum-Johnson, Kristin E.; Kim, Young-Mo; Kyle, Jennifer E.; Matzke, Melissa M.; Shukla, Anil K.; Chu, Rosalie K.; Schepmoes, Athena A.; Jacobs, Jon M.; Baric, Ralph S.; Webb-Robertson, Bobbie-Jo; Smith, Richard D.; Metz, Thomas O.; Chia, Nicholas

2016-05-03

Integrative multi-omics analyses can empower more effective investigation and complete understanding of complex biological systems. Despite recent advances in a range of omics analyses, multi-omic measurements of the same sample are still challenging and current methods have not been well evaluated in terms of reproducibility and broad applicability. Here we adapted a solvent-based method, widely applied for extracting lipids and metabolites, to add proteomics to mass spectrometry-based multi-omics measurements. Themetabolite,protein, andlipidextraction (MPLEx) protocol proved to be robust and applicable to a diverse set of sample types, including cell cultures, microbial communities, and tissues. To illustrate the utility of this protocol, an integrative multi-omics analysis was performed using a lung epithelial cell line infected with Middle East respiratory syndrome coronavirus, which showed the impact of this virus on the host glycolytic pathway and also suggested a role for lipids during infection. The MPLEx method is a simple, fast, and robust protocol that can be applied for integrative multi-omic measurements from diverse sample types (e.g., environmental,in vitro, and clinical).

IMPORTANCEIn systems biology studies, the integration of multiple omics measurements (i.e., genomics, transcriptomics, proteomics, metabolomics, and lipidomics) has been shown to provide a more complete and informative view of biological pathways. Thus, the prospect of extracting different types of molecules (e.g., DNAs, RNAs, proteins, and metabolites) and performing multiple omics measurements on single samples is very attractive, but such studies are challenging due to the fact that the extraction conditions differ according to the molecule type. Here, we adapted an organic solvent-based extraction method that demonstrated

Efficacy of Ballast Water Treatment Systems: A Report by the EPA Science Advisory Board

Science.gov (United States)

2011-07-12

followed during testing, are needed. In addition, the EPA 3 Protists refers to various one-celled...should develop metrics and methods appropriate for compliance monitoring and enforcement as soon as possible. Limits for selected protists < 10 µm...as “ protist -sized” organisms, or organisms ≥10 µm and < 50 µm in minimum dimension), and the ETV Protocol provides examples with a more stringent

GAC-EPA

CERN Document Server

GAC-EPA

2013-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 1er octobre de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 5 novembre et 3 décembre 2013. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

GAC-EPA

CERN Multimedia

GAC-EPA

2016-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : le mardi 1er novembre de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel. La permanence suivante aura lieu le mardi 29 novembre 2016. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

GAC-EPA

CERN Document Server

GAC-EPA

2016-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 4 octobre de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 1er et 29 novembre 2016. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

GAC-EPA

CERN Multimedia

GAC-EPA

2015-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 3 novembre de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel La permanence suivante aura lieu le mardi 1er décembre 2015. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

One Sample, One Shot - Evaluation of sample preparation protocols for the mass spectrometric proteome analysis of human bile fluid without extensive fractionation.

Science.gov (United States)

Megger, Dominik A; Padden, Juliet; Rosowski, Kristin; Uszkoreit, Julian; Bracht, Thilo; Eisenacher, Martin; Gerges, Christian; Neuhaus, Horst; Schumacher, Brigitte; Schlaak, Jörg F; Sitek, Barbara

2017-02-10

The proteome analysis of bile fluid represents a promising strategy to identify biomarker candidates for various diseases of the hepatobiliary system. However, to obtain substantive results in biomarker discovery studies large patient cohorts necessarily need to be analyzed. Consequently, this would lead to an unmanageable number of samples to be analyzed if sample preparation protocols with extensive fractionation methods are applied. Hence, the performance of simple workflows allowing for "one sample, one shot" experiments have been evaluated in this study. In detail, sixteen different protocols implying modifications at the stages of desalting, delipidation, deglycosylation and tryptic digestion have been examined. Each method has been individually evaluated regarding various performance criteria and comparative analyses have been conducted to uncover possible complementarities. Here, the best performance in terms of proteome coverage has been assessed for a combination of acetone precipitation with in-gel digestion. Finally, a mapping of all obtained protein identifications with putative biomarkers for hepatocellular carcinoma (HCC) and cholangiocellular carcinoma (CCC) revealed several proteins easily detectable in bile fluid. These results can build the basis for future studies with large and well-defined patient cohorts in a more disease-related context. Human bile fluid is a proximal body fluid and supposed to be a potential source of disease markers. However, due to its biochemical composition, the proteome analysis of bile fluid still represents a challenging task and is therefore mostly conducted using extensive fractionation procedures. This in turn leads to a high number of mass spectrometric measurements for one biological sample. Considering the fact that in order to overcome the biological variability a high number of biological samples needs to be analyzed in biomarker discovery studies, this leads to the dilemma of an unmanageable number of

Supplementation of docosahexaenoic acid (DHA) / Eicosapentaenoic acid (EPA) in a ratio of 1/1.3 during the last trimester of pregnancy results in EPA accumulation in cord blood.

Science.gov (United States)

Büyükuslu, Nihal; Ovalı, Sema; Altuntaş, Şükriye Leyla; Batırel, Saime; Yiğit, Pakize; Garipağaoğlu, Muazzez

2017-10-01

Omega-3 fatty acids (n-3 FA), specifically DHA, are associated with fetal growth and development. We aimed to determine the levels of DHA and EPA in cord serum after n-3 FA supplementation during the last trimester of pregnancy. Among 55 women, 23 were administered daily one capsule of n-3 FA supplement, involving DHA/EPA in a ratio of 1/1.3. Twenty nine women were enrolled as control group. Blood samples were collected at 22-24 weeks of gestation and at delivery. Fatty acids were analyzed with the method of GC-MS. Cord DHA level increased and EPA level decreased in both groups between the days of 22-24 and delivery. However, decrease in cord EPA level was significant in control group (p 0.05). Supplementation of DHA/EPA in a ratio of 1/1.3 during the last trimester of pregnancy caused higher cord EPA level compared to control group indicating an accumulation in umbilical cord. Copyright © 2017 Elsevier Ltd. All rights reserved.

EPA Linked Open Data (Collection)

Data.gov (United States)

U.S. Environmental Protection Agency — This is a collection item referencing the following EPA Linked Data resources: - EPA Facility Registry Service (FRS) - EPA Substance Registry Service (SRS) -...

EPA Library Network Communication Strategies

Science.gov (United States)

To establish Agency-wide procedures for the EPA National Library Network libraries to communicate, using a range of established mechanisms, with other EPA libraries, EPA staff, organizations and the public.

GAC-EPA

CERN Multimedia

GAC-EPA

2016-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 5 avril de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 3 mai, 7 juin, 6 septembre, 4 octobre, 1er et 29 novembre décembre 2016. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

GAC-EPA

CERN Document Server

GAC-EPA

2016-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 3 mai de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 7 juin, 6 septembre, 4 octobre, 1er et 29 novembre décembre 2016. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

GAC-EPA

CERN Document Server

GAC-EPA

2016-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 5 avril de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 3 mai, 7 juin, 6 septembre, 4 octobre, 1er et 29 novembre 2016. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

GAC-EPA

CERN Multimedia

GAC-EPA

2016-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 1er mars de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 5 avril, 3 mai, 7 juin, 6 septembre, 4 octobre, 1er et 29 novembre 2016. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

The UK Biobank sample handling and storage protocol for the collection, processing and archiving of human blood and urine.

Science.gov (United States)

Elliott, Paul; Peakman, Tim C

2008-04-01

UK Biobank is a large prospective study in the UK to investigate the role of genetic factors, environmental exposures and lifestyle in the causes of major diseases of late and middle age. Extensive data and biological samples are being collected from 500,000 participants aged between 40 and 69 years. The biological samples that are collected and how they are processed and stored will have a major impact on the future scientific usefulness of the UK Biobank resource. The aim of the UK Biobank sample handling and storage protocol is to specify methods for the collection and storage of participant samples that give maximum scientific return within the available budget. Processing or storage methods that, as far as can be predicted, will preclude current or future assays have been avoided. The protocol was developed through a review of the literature on sample handling and processing, wide consultation within the academic community and peer review. Protocol development addressed which samples should be collected, how and when they should be processed and how the processed samples should be stored to ensure their long-term integrity. The recommended protocol was extensively tested in a series of validation studies. UK Biobank collects about 45 ml blood and 9 ml of urine with minimal local processing from each participant using the vacutainer system. A variety of preservatives, anti-coagulants and clot accelerators is used appropriate to the expected end use of the samples. Collection of other material (hair, nails, saliva and faeces) was also considered but rejected for the full cohort. Blood and urine samples from participants are transported overnight by commercial courier to a central laboratory where they are processed and aliquots of urine, plasma, serum, white cells and red cells stored in ultra-low temperature archives. Aliquots of whole blood are also stored for potential future production of immortalized cell lines. A standard panel of haematology assays is

GAC-EPA

CERN Multimedia

GAC-EPA

2015-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 3 mars de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 7 avril, 5 mai, 2 juin, 1er septembre, 6 octobre, 3 novembre et 1er décembre 2013. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

GAC-EPA

CERN Multimedia

GAC-EPA

2016-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 2 février de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 1er mars, 5 avril, 3 mai, 7 juin, 6 septembre, 4 octobre, 1er et 29 novembre 2016. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

EPA for Businesses and Non-Profits

Science.gov (United States)

Information and links to EPA web pages that are meant to help businesses and non-profits adhere to EPA regulations and otherwise protect the environment, take advantage of opportunities to collaborate with the EPA, and find training EPA training programs.

Advanced Curation Protocols for Mars Returned Sample Handling

Science.gov (United States)

Bell, M.; Mickelson, E.; Lindstrom, D.; Allton, J.

Introduction: Johnson Space Center has over 30 years experience handling precious samples which include Lunar rocks and Antarctic meteorites. However, we recognize that future curation of samples from such missions as Genesis, Stardust, and Mars S mple Return, will require a high degree of biosafety combined witha extremely low levels of inorganic, organic, and biological contamination. To satisfy these requirements, research in the JSC Advanced Curation Lab is currently focused toward two major areas: preliminary examination techniques and cleaning and verification techniques . Preliminary Examination Techniques : In order to minimize the number of paths for contamination we are exploring the synergy between human &robotic sample handling in a controlled environment to help determine the limits of clean curation. Within the Advanced Curation Laboratory is a prototype, next-generation glovebox, which contains a robotic micromanipulator. The remotely operated manipulator has six degrees-of- freedom and can be programmed to perform repetitive sample handling tasks. Protocols are being tested and developed to perform curation tasks such as rock splitting, weighing, imaging, and storing. Techniques for sample transfer enabling more detailed remote examination without compromising the integrity of sample science are also being developed . The glovebox is equipped with a rapid transfer port through which samples can be passed without exposure. The transfer is accomplished by using a unique seal and engagement system which allows passage between containers while maintaining a first seal to the outside environment and a second seal to prevent the outside of the container cover and port door from becoming contaminated by the material being transferred. Cleaning and Verification Techniques: As part of the contamination control effort, innovative cleaning techniques are being identified and evaluated in conjunction with sensitive cleanliness verification methods. Towards this

EPA Administrative Enforcement Dockets

Data.gov (United States)

U.S. Environmental Protection Agency — The EPA Administrative Enforcement Dockets database contains the electronic dockets for administrative penalty cases filed by EPA Regions and Headquarters. Visitors...

A ton is not always a ton: A road-test of landfill, manure, and afforestation/reforestation offset protocols in the U.S. carbon market

International Nuclear Information System (INIS)

Lee, Carrie M.; Lazarus, Michael; Smith, Gordon R.; Todd, Kimberly; Weitz, Melissa

2013-01-01

Highlights: • Protocols are the foundation of an offset program. • Using sample projects, we “road test” landfill, manure and afforestation protocols from 5 programs. • For a given project, we find large variation in the volume of offsets generated. • Harmonization of protocols can increase the likelihood that “a ton is a ton”. • Harmonization can enhance prospects for linking emission trading systems. -- Abstract: The outcome of recent international climate negotiations suggests we are headed toward a more fragmented carbon market, with multiple emission trading and offset programs operating in parallel. To effectively harmonize and link across programs, it will be important to ensure that across offset programs and protocols that a “ton is a ton”. In this article, we consider how sample offsets projects in the U.S. carbon market are treated across protocols from five programs: the Clean Development Mechanism, Climate Action Reserve, Chicago Climate Exchange, Regional Greenhouse Gas Initiative, and the U.S. EPA's former program, Climate Leaders. We find that differences among protocols for landfill methane, manure management, and afforestation/reforestation project types in accounting boundary definitions, baseline setting methods, measurement rules, emission factors, and discounts lead to differences in offsets credited that are often significant (e.g. greater than 50%). We suggest opportunities for modification and harmonization of protocols that can improve offset quality and credibility and enhance prospects for future linking of trading units and systems

An On-Target Desalting and Concentration Sample Preparation Protocol for MALDI-MS and MS/MS Analysis

DEFF Research Database (Denmark)

Zhang, Xumin; Wang, Quanhui; Lou, Xiaomin

2012-01-01

2DE coupled with MALDI-MS is one of the most widely used and powerful analytic technologies in proteomics study. The MALDI sample preparation method has been developed and optimized towards the combination of simplicity, sample-cleaning, and sample concentration since its introduction. Here we...... present a protocol of the so-called Sample loading, Matrix loading, and on-target Wash (SMW) method which fulfills the three criteria by taking advantage of the AnchorChip™ targets. Our method is extremely simple and no pre-desalting or concentration is needed when dealing with samples prepared from 2DE...

US EPA Region 4 Brownfields

Science.gov (United States)

To improve public health and the environment, the United States Environmental Protection Agency (USEPA) collects information about facilities, sites, or places subject to environmental regulation or of environmental interest. Through the Geospatial Data Download Service, the public is now able to download the EPA Geodata shapefile containing facility and site information from EPA's national program systems. The file is Internet accessible from the Envirofacts Web site (https://www3.epa.gov/enviro/). The data may be used with geospatial mapping applications. (Note: The shapefile omits facilities without latitude/longitude coordinates.) The EPA Geospatial Data contains the name, location (latitude/longitude), and EPA program information about specific facilities and sites. In addition, the file contains a Uniform Resource Locator (URL), which allows mapping applications to present an option to users to access additional EPA data resources on a specific facility or site. This dataset shows Brownfields listed in the 2012 Facility Registry System.

[Sampling and measurement methods of the protocol design of the China Nine-Province Survey for blindness, visual impairment and cataract surgery].

Science.gov (United States)

Zhao, Jia-liang; Wang, Yu; Gao, Xue-cheng; Ellwein, Leon B; Liu, Hu

2011-09-01

To design the protocol of the China nine-province survey for blindness, visual impairment and cataract surgery to evaluate the prevalence and main causes of blindness and visual impairment, and the prevalence and outcomes of the cataract surgery. The protocol design was began after accepting the task for the national survey for blindness, visual impairment and cataract surgery from the Department of Medicine, Ministry of Health, China, in November, 2005. The protocol in Beijing Shunyi Eye Study in 1996 and Guangdong Doumen County Eye Study in 1997, both supported by World Health Organization, was taken as the basis for the protocol design. The relative experts were invited to discuss and prove the draft protocol. An international advisor committee was established to examine and approve the draft protocol. Finally, the survey protocol was checked and approved by the Department of Medicine, Ministry of Health, China and Prevention Program of Blindness and Deafness, WHO. The survey protocol was designed according to the characteristics and the scale of the survey. The contents of the protocol included determination of target population and survey sites, calculation of the sample size, design of the random sampling, composition and organization of the survey teams, determination of the examinee, the flowchart of the field work, survey items and methods, diagnostic criteria of blindness and moderate and sever visual impairment, the measures of the quality control, the methods of the data management. The designed protocol became the standard and practical protocol for the survey to evaluate the prevalence and main causes of blindness and visual impairment, and the prevalence and outcomes of the cataract surgery.

GAC-EPA

CERN Multimedia

GAC-EPA

2017-01-01

Le GAC organise des permanences avec entretiens individuels qui se tiennent le dernier mardi de chaque mois, sauf en juin, juillet et décembre. La prochaine permanence se tiendra le : Mardi 31 janvier de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 28 février, 28 mars, 25 avril, 30 mai, 29 août, 26 septembre, 31 octobre et 28 novembre 2017. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. e-mail : gac-epa@gac-epa.org.

Feasibility study for the computerized automation of the Annapolis Field Office of EPA region III

International Nuclear Information System (INIS)

Ames, H.S.; Barton, G.W. Jr.; Bystroff, R.I.; Crawford, R.W.; Kray, A.M.; Maples, M.D.

1976-08-01

This report describes a feasibility study for computerized automation of the Annapolis Field Office (AFO) of EPA's Region III. The AFO laboratory provides analytical support for a number of EPA divisions; its primary function at present is analysis of water samples from rivers, estuaries, and the ocean in the Chesapeake Bay area. Automation of the AFO laboratory is found to be not only feasible but also highly desirable. An automation system is proposed which will give major improvements in analytical capacity, quality control, sample management, and reporting capabilities. This system is similar to the LLL-developed automation systems already installed at other EPA laboratories, with modifications specific to the needs of the AFO laboratory and the addition of sample file control. It is estimated that the initial cost of the system, nearly $300,000, would be recouped in about three years by virtue of the increased capacity and efficiency of operation

Establishment of a protocol for the gene expression analysis of laser microdissected rat kidney samples with affymetrix genechips

International Nuclear Information System (INIS)

Stemmer, Kerstin; Ellinger-Ziegelbauer, Heidrun; Lotz, Kerstin; Ahr, Hans-J.; Dietrich, Daniel R.

2006-01-01

Laser microdissection in conjunction with microarray technology allows selective isolation and analysis of specific cell populations, e.g., preneoplastic renal lesions. To date, only limited information is available on sample preparation and preservation techniques that result in both optimal histomorphological preservation of sections and high-quality RNA for microarray analysis. Furthermore, amplification of minute amounts of RNA from microdissected renal samples allowing analysis with genechips has only scantily been addressed to date. The objective of this study was therefore to establish a reliable and reproducible protocol for laser microdissection in conjunction with microarray technology using kidney tissue from Eker rats p.o. treated for 7 days and 6 months with 10 and 1 mg Aristolochic acid/kg bw, respectively. Kidney tissues were preserved in RNAlater or snap frozen. Cryosections were cut and stained with either H and E or cresyl violet for subsequent morphological and RNA quality assessment and laser microdissection. RNA quality was comparable in snap frozen and RNAlater-preserved samples, however, the histomorphological preservation of renal sections was much better following cryopreservation. Moreover, the different staining techniques in combination with sample processing time at room temperature can have an influence on RNA quality. Different RNA amplification protocols were shown to have an impact on gene expression profiles as demonstrated with Affymetrix Rat Genome 230 2 .0 arrays. Considering all the parameters analyzed in this study, a protocol for RNA isolation from laser microdissected samples with subsequent Affymetrix chip hybridization was established that was also successfully applied to preneoplastic lesions laser microdissected from Aristolochic acid-treated rats

EPA Office of Water (OW): Fish Consumption Advisories and Fish Tissue Sampling Stations NHDPlus Indexed Datasets

Data.gov (United States)

U.S. Environmental Protection Agency — The Fish Consumption Advisories dataset contains information on Fish Advisory events that have been indexed to the EPA Office of Water NHDPlus v2.1 hydrology and...

EPA Method 1615. Measurement of Enterovirus and Norovirus ...

Science.gov (United States)

A standardized method is required when national studies on virus occurrence in environmental and drinking waters utilize multiple analytical laboratories. The U.S Environmental Protection Agency’s (USEPA) Method 1615 was developed with the goal of providing such a standard for measuring Enterovirus and Norovirus in these waters. Virus is concentrated from water using an electropositive filter, eluted from the filter surface with beef extract, and then concentrated further using organic flocculation. Herein we present the protocol from Method 1615 for filter elution, secondary concentration, and measurement of total culturable viruses. A portion of the concentrated eluate from each sample is inoculated onto ten replicate flasks of Buffalo Green Monkey kidney cells. The number of flasks demonstrating cytopathic effects is used to quantify the most probable number (MPN) of infectious units per liter. The method uses a number of quality controls to increase data quality and to reduce interlaboratory and intralaboratory variation. Laboratories must meet defined performance standards. Method 1615 was evaluated by examining virus recovery from reagent-grade and ground waters seeded with Sabin poliovirus type 3. Mean poliovirus recoveries with the total culturable assay were 111% in reagent grade water and 58% in groundwaters. EPA Method 1615 is being used by a number of national and international labs. This paper and the accompanying video will provide training oppo

EPA's Information Architecture and Web Taxonomy

Science.gov (United States)

EPA's Information Architecture creates a topical organization of our website, instead of an ownership-based organization. The EPA Web Taxonomy allows audiences easy access to relevant information from EPA programs, by using a common vocabulary.

Comparative analysis of five DNA isolation protocols and three drying methods for leaves samples of Nectandra megapotamica (Spreng. Mez

Directory of Open Access Journals (Sweden)

Leonardo Severo da Costa

2016-06-01

Full Text Available The aim of the study was to establish a DNA isolation protocol Nectandra megapotamica (Spreng. Mez., able to obtain samples of high yield and quality for use in genomic analysis. A commercial kit and four classical methods of DNA extraction were tested, including three cetyltrimethylammonium bromide (CTAB-based and one sodium dodecyl sulfate (SDS-based methods. Three drying methods for leaves samples were also evaluated including drying at room temperature (RT, in an oven at 40ºC (S40, and in a microwave oven (FMO. The DNA solutions obtained from different types of leaves samples using the five protocols were assessed in terms of cost, execution time, and quality and yield of extracted DNA. The commercial kit did not extract DNA with sufficient quantity or quality for successful PCR reactions. Among the classic methods, only the protocols of Dellaporta and of Khanuja yielded DNA extractions for all three types of foliar samples that resulted in successful PCR reactions and subsequent enzyme restriction assays. Based on the evaluated variables, the most appropriate DNA extraction method for Nectandra megapotamica (Spreng. Mez. was that of Dellaporta, regardless of the method used to dry the samples. The selected method has a relatively low cost and total execution time. Moreover, the quality and quantity of DNA extracted using this method was sufficient for DNA sequence amplification using PCR reactions and to get restriction fragments.

US EPA Region 4 RMP Facilities

Science.gov (United States)

To improve public health and the environment, the United States Environmental Protection Agency (USEPA) collects information about facilities, sites, or places subject to environmental regulation or of environmental interest. Through the Geospatial Data Download Service, the public is now able to download the EPA Geodata shapefile containing facility and site information from EPA's national program systems. The file is Internet accessible from the Envirofacts Web site (http://www.epa.gov/enviro). The data may be used with geospatial mapping applications. (Note: The shapefile omits facilities without latitude/longitude coordinates.) The EPA Geospatial Data contains the name, location (latitude/longitude), and EPA program information about specific facilities and sites. In addition, the file contains a Uniform Resource Locator (URL), which allows mapping applications to present an option to users to access additional EPA data resources on a specific facility or site.

EPA scientific integrity policy draft

Science.gov (United States)

Showstack, Randy

2011-08-01

The U.S. Environmental Protection Agency (EPA) issued its draft scientific integrity policy on 5 August. The draft policy addresses scientific ethical standards, communications with the public, the use of advisory committees and peer review, and professional development. The draft policy was developed by an ad hoc group of EPA senior staff and scientists in response to a December 2010 memorandum on scientific integrity from the White House Office of Science and Technology Policy. The agency is accepting public comments on the draft through 6 September; comments should be sent to osa.staff@epa.gov. For more information, see http://www.epa.gov/stpc/pdfs/draft-scientific-integrity-policy-aug2011.pdf.

US EPA overview

International Nuclear Information System (INIS)

Rowe, W.D.

1976-01-01

EPA believes that effective and efficient solutions to problems in all radioactive waste disposal areas will require close coordination and cooperation among all agencies involved. In this regard, EPA already has participated in meetings with the Energy Research and Development Administration, the Nuclear Regulatory Commission, the Council on Environmental Quality, the U.S. Geological Survey, and the Office of Management and Budget to lay the groundwork for the development of a consolidated national radioactive waste disposal plan. The EPA program is directed first toward developing goals and requirements; and then, in cooperation with the public, industry, the States and Federal agencies, towards determining by what means these goals can be achieved for each waste management option. In addition, the program will develop criteria for determining when the goals of the waste management options have been achieved. In summary, EPA will provide fundamental environmental criteria and generally applicable environmental standards for permanent disposal of high level radwastes. Concurrently, ERDA will develop the necessary technology; and NRC will conduct necessary studies, develop waste-related regulations, and license specific sites and methods of control. Together, we will be able to manage the disposal of the Nation's radioactive waste in an environmentally adequate manner

Development of a new protocol for rapid bacterial identification and susceptibility testing directly from urine samples.

Science.gov (United States)

Zboromyrska, Y; Rubio, E; Alejo, I; Vergara, A; Mons, A; Campo, I; Bosch, J; Marco, F; Vila, J

2016-06-01

The current gold standard method for the diagnosis of urinary tract infections (UTI) is urine culture that requires 18-48 h for the identification of the causative microorganisms and an additional 24 h until the results of antimicrobial susceptibility testing (AST) are available. The aim of this study was to shorten the time of urine sample processing by a combination of flow cytometry for screening and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for bacterial identification followed by AST directly from urine. The study was divided into two parts. During the first part, 675 urine samples were processed by a flow cytometry device and a cut-off value of bacterial count was determined to select samples for direct identification by MALDI-TOF-MS at ≥5 × 10(6) bacteria/mL. During the second part, 163 of 1029 processed samples reached the cut-off value. The sample preparation protocol for direct identification included two centrifugation and two washing steps. Direct AST was performed by the disc diffusion method if a reliable direct identification was obtained. Direct MALDI-TOF-MS identification was performed in 140 urine samples; 125 of the samples were positive by urine culture, 12 were contaminated and 3 were negative. Reliable direct identification was obtained in 108 (86.4%) of the 125 positive samples. AST was performed in 102 identified samples, and the results were fully concordant with the routine method among 83 monomicrobial infections. In conclusion, the turnaround time of the protocol described to diagnose UTI was about 1 h for microbial identification and 18-24 h for AST. Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

EPA perspective on federal facility agreements

International Nuclear Information System (INIS)

Grundler, C.

1988-01-01

Although DOE's image with Congress and the media concerning environmental compliance may be poor, EPA sees the Department's recent attitude toward the environment as good. DOE and EPA must continue to move forward. In particular, EPA would like to emphasize less study of a problem and more clean-up. Strong, enforceable agreements will allow this goal to be met by letting EPA take more risks in its decision making. Currently EPA is developing an enforcement strategy for Federal facilities. This strategy will address identifying Federal facilities of concern, increasing enforcement and compliance monitoring activities at those facilities, implementing the model agreements, resource planning, and the establishment of an Agency Management System for Federal facilities. There are over 1000 Federal facilities which are listed on the EPA compliance docket. Over 200 Federal facilities are expected to be included on the NPL. Increased EPA attention may increase the ability of the various Federal agencies to obtain the necessary funding. Another subject being addressed by EPA is the liability of government contractors under the environmental statutes. The Agency is developing a GoCo enforcement strategy. In the hazardous waste enforcement program, three criteria are being considered for determining when to proceed against a contractor: Degree of contractor control over the hazardous waste management activity. Who is actually performing the work, and Degree of Departmental cooperation

EPA's analytical methods for water: The next generation

International Nuclear Information System (INIS)

Hites, R.A.; Budde, W.L.

1991-01-01

By the late 1970s, it had become clear to EPA that organic compounds were polluting many of the nation's waters. By 1977, as a result of a lawsuit by several environmentally concerned plaintiffs, EPA had focused on a list of 114 'priority' organic pollutants. Its long-term goal was the regulation of specific compounds that were found to pose significant environmental problems, a daunting task. Tens of thousands of samples needed to be measured by hundreds of different laboratories. Clearly, there were concerns about the comparability of data among laboratories. The result was a series of laboratory-based analytical 'methods.' These EPA methods are detailed, step-by-step directions (recipes) that describe everything the analyst needs to know to complete a satisfactory analysis. During the 1970s the first set of methods was developed; this was the '600 series' for the analysis of organic compounds in wastewater. In 1979 and the 1980s, a set of '500 series' methods, focusing on drinking water, was developed. By now, many of the 500 and 600 series methods are in widespread use, and it is clear that there are considerably overlaps among the methods in terms of both procedures and analytes. Indiana University was asked by EPA to consider the question, 'Is it possible to revise or eliminate some of the 500 and 600 series methods and effect a savings of time and money?' This and related questions were studied and recommendations were developed

Fast filtration sampling protocol for mammalian suspension cells tailored for phosphometabolome profiling by capillary ion chromatography - tandem mass spectrometry.

Science.gov (United States)

Kvitvang, Hans F N; Bruheim, Per

2015-08-15

Capillary ion chromatography (capIC) is the premium separation technology for low molecular phosphometabolites and nucleotides in biological extracts. Removal of excessive amounts of salt during sample preparation stages is a prerequisite to enable high quality capIC separation in combination with reproducible and sensitive MS detection. Existing sampling protocols for mammalian cells used for GC-MS and LC-MS metabolic profiling can therefore not be directly applied to capIC separations. Here, the development of a fast filtration sampling protocol for mammalian suspension cells tailored for quantitative profiling of the phosphometabolome on capIC-MS/MS is presented. The whole procedure from sampling the culture to transfer of filter to quenching and extraction solution takes less than 10s. To prevent leakage it is critical that a low vacuum pressure is applied, and satisfactorily reproducibility was only obtained by usage of a vacuum pressure controlling device. A vacuum of 60mbar was optimal for filtration of multiple myeloma Jjn-3 cell cultures through 5μm polyvinylidene (PVDF) filters. A quick deionized water (DI-water) rinse step prior to extraction was tested, and significantly higher metabolite yields were obtained during capIC-MS/MS analyses in this extract compared to extracts prepared by saline and reduced saline (25%) washing steps only. In addition, chromatographic performance was dramatically improved. Thus, it was verified that a quick DI-water rinse is tolerated by the cells and can be included as the final stage during filtration. Over 30 metabolites were quantitated in JJN-3 cell extracts by using the optimized sampling protocol with subsequent capIC-MS/MS analysis, and up to 2 million cells can be used in a single filtration step for the chosen filter and vacuum pressure. The technical set-up is also highly advantageous for microbial metabolome filtration protocols after optimization of vacuum pressure and washing solutions, and the reduced salt

Open Letter from the GAC-EPA to the Chairman of the PFGB EPA

CERN Multimedia

GAC-EPA

2012-01-01

Following the publication by the Chairman of the Pension Fund Governing Board of  the Spring report of the Pension Fund in the CERN Bulletin issue dated 25 July 2012 (Nos 30 & 31), the GAC-EPA has reacted through an open letter. See www.gac-epa.org under Announcement.

Cheminformatics Analysis of EPA ToxCast Chemical Libraries ...

Science.gov (United States)

An important goal of toxicology research is the development of robust methods that use in vitro and chemical structure information to predict in vivo toxicity endpoints. The US EPA ToxCast program is addressing this goal using ~600 in vitro assays to create bioactivity profiles on a set of 320 compounds, mostly pesticide actives, that have well characterized in vivo toxicity. These 320 compounds (EPA-320 set evaluated in Phase I of ToxCast) are a subset of a much larger set of ~10,000 candidates that are of interest to the EPA (called here EPA-10K). Predictive models of in vivo toxicity are being constructed from the in vitro assay data on the EPA-320 chemical set. These models require validation on additional chemicals prior to wide acceptance, and this will be carried out by evaluating compounds from EPA-10K in Phase II of ToxCast. We have used cheminformatics approaches including clustering, data visualization, and QSAR to develop models for EPA-320 that could help prioritizing EPA-10K validation chemicals. Both chemical descriptors, as well as calculated physicochemical properties have been used. Compounds from EPA-10K are prioritized based on their similarity to EPA-320 using different similarity metrics, with similarity thresholds defining the domain of applicability for the predictive models built for EPA-320 set. In addition, prioritized lists of compounds of increasing dissimilarity from the EPA-320 have been produced, to test the ability of the EPA-320

EPA Communications Stylebook: Writing Guide

Science.gov (United States)

For the most part, EPA follows the Associated Press (AP) Stylebook. Other requirements of basic punctuation and grammar and usage in EPA writing modify, supplement, or in some cases reiterate AP style.

STUDY ON THE THERMAL STABILITY OF EPA AND DHA IN MUJAHIR (Oreochromis mossambicus FISH OIL

Directory of Open Access Journals (Sweden)

Ngatidjo Hadipranoto

2010-06-01

Full Text Available EPA (Eicosapentaenoic acid and DHA (Docosahexaenoic acid content in common fresh water fish : mujahir (Oreochromis mossambicus after indirect heating were analysed. The aims of this study were to determine the effect of indirect heating process and α-tocopherol additions on both fatty acid stability.Lipids content in the mujahir fillets were extracted by Folch method using chloroform-metanol (2:1 mixture. Fatty acids in fish oil were converted to fatty acid methyl esters and then injected into gas chromatography to determine the EPA and DHA concentration. Operating condition of gas chromatography were programmed as follows: injection port temperature at 270 oC, detector at 280 oC, initial column temperature at 200 oC, and the final at 280 oC, the carrier gas was helium with flow rate of 10 ml per minute and temperature of column was increased gradually at 10 oC per minute. The effect of α-tocopherol addition on the stability of EPA and DHA was studied by adding α-tocopherol at 50 to 200 mg per kilogram sample before indirect heating process was carried out.The analysis of mujahir fish oil showed that the content of EPA and DHA in 100 grams fresh sample was 105 and 406,5 mg respectivelly. Indirect heating caused the EPA and DHA content decreased significantly. The addition of α-tocopherol results in a positive corelation between α-tocopherol concentration added and the decrease of EPA and DHA content during the heating process.   Keywords: fatty acid, eicosapentaenoic acid, docosahexaenoic acid

EPA Alternative Dispute Resolution Contacts

Science.gov (United States)

The success of EPA's ADR efforts depends on a network of talented and experienced professionals in Headquarters offices and EPA Regions. For Agency-wide ADR information, please contact the Conflict Prevention and Resolution Center.

HUBUNGAN KECUKUPAN ASAM EIKOSAPENTANOAT (EPA, ASAM DOKOSAHEKSANOAT (DHA IKAN DAN STATUS GIZI DENGAN PRESTASI BELAJAR SISWA

Directory of Open Access Journals (Sweden)

Siti Zulaihah

2012-04-01

Full Text Available ABSTRACT   Background: Fish contain of high protein, EPA, DHA needed for the formation of brain cell and improving intelligence. Consuming fish and other sea food make healthy and improve the brain ability to reach optimum study achievement. In 2003, fish consumption in Indonesia is still low 24,67kg/capita/year. Based on BPS 2002, fish consumption in Semarang is 5,38%. The fish consumption has a big influence on nutrition sufficiency especially EPA and DHA, nutrition status and attaining healthy and smart Indonesian human resources. Goal: To analyze the relationship between fish meal frequency, fish EPA and DHA recommended and nutrition status with student's study achievement. Method: The research used survey method, analytical research, and cross -sectional time approach. This research was conducted on September-October 2004. Sample was 100 subject of SD Taqwiyatui Wathon (grade IV are 54 person, grade V are 46 person by using Stratified Random Sampling method. The data preparation used NUTRISOFT. Result: Fish frequently consumed by responden was bandeng (Chanos chanos 5%, tongkol (Euthynnus allitteratus rafmescue 4%, kembung (Scomber kanoguria russei 1% and mujair (Tilapia mossambica 1 %. EPA, DHA % RDA defisit 62%, normal nutritional status 93% and average category of study achievement 55%. There was relation between fish meal frequency and fish EPA, DHA % RDA (ρ=0,000, there was no relation between fish meal frequency and nutritional status (ρ=0,213, there was relation between fish meal frequency and study achievement (ρ=0,000, there was relation between fish EPA, DHA recommendation and study achievement (ρ=0,000, and there was no relation between nutrition status and study achievement (ρ=0.378. Based on Pearson correlation test, there was no relation between fish EPA, DHA recommendation and nutritional status (ρ=0,000. Conclution: Students with frequent fish consumption and high RDA of EPA, DHA % RDA showed better study achievement

Electron-Positron Accumulator (EPA)

CERN Multimedia

Photographic Service

1986-01-01

After acceleration in the low-current linac LIL-W, the electrons and positrons are accumulated in EPA to obtain a sufficient intensity and a suitable time-structure, before being passed on to the PS for further acceleration to 3.5 GeV. Electrons circulate from right to left, positrons in the other direction. Dipole bending magnets are red, focusing quadrupoles blue, sextupoles for chromaticity-control orange. The vertical tube at the left of the picture belongs to an optical transport system carrying the synchrotron radiation to detectors for beam size measurement. Construction of EPA was completed in spring 1986. LIL-W and EPA were conceived for an energy of 600 MeV, but operation was limited to 500 MeV.

Integration of GC-MSD and ER-Calux® assay into a single protocol for determining steroid estrogens in environmental samples.

Science.gov (United States)

Avberšek, Miha; Žegura, Bojana; Filipič, Metka; Heath, Ester

2011-11-01

There are many published studies that use either chemical or biological methods to investigate steroid estrogens in the aquatic environment, but rarer are those that combine both. In this study, gas chromatography with mass selective detection (GC-MSD) and the ER-Calux(®) estrogenicity assay were integrated into a single protocol for simultaneous determination of natural (estrone--E1, 17β-estradiol--E2, estriol--E3) and synthetic (17α-ethinylestradiol--EE2) steroid estrogens concentrations and the total estrogenic potential of environmental samples. For integration purposes, several solvents were investigated and the commonly used dimethyl sulphoxide (DMSO) in the ER-Calux(®) assay was replaced by ethyl acetate, which is more compatible with gas chromatography and enables the same sample to be analysed by both GC-MSD and the ER-Calux(®) assay. The integrated protocol was initially tested using a standard mixture of estrogens. The results for pure standards showed that the estrogenicity calculated on the basis of GC-MSD and the ER-Calux(®) assay exhibited good correlation (r(2)=0.96; α=0.94). The result remained the same when spiked waste water extracts were tested (r(2)=0.92, α=1.02). When applied to real waste water influent and effluent samples the results proved (r(2)=0.93; α=0.99) the applicability of the protocol. The main advantages of this newly developed protocol are simple sample handling for both methods, and reduced material consumption and labour. In addition, it can be applied as either a complete or sequential analysis where the ER-Calux(®) assay is used as a pre-screening method prior to the chemical analysis. Copyright © 2011 Elsevier B.V. All rights reserved.

Report: EPA Needs to Improve Internal Controls to Increase Cost Recovery

Science.gov (United States)

Report #09-P-0144, April 27, 2009. Within a sample of removal actions we reviewed, EPA collected from responsible parties approximately 11 percent ($31.4 of $294.5 million) of the Federal Government’s costs for conducting the removal actions.

Toxoplasma gondii and pre-treatment protocols for polymerase chain reaction analysis of milk samples: a field trial in sheep from Southern Italy

Directory of Open Access Journals (Sweden)

Alice Vismarra

2017-02-01

Full Text Available Toxoplasmosis is a zoonotic disease caused by the protozoan Toxoplasma gondii. Ingestion of raw milk has been suggested as a risk for transmission to humans. Here the authors evaluated pre-treatment protocols for DNA extraction on T. gondii tachyzoite-spiked sheep milk with the aim of identifying the method that resulted in the most rapid and reliable polymerase chain reaction (PCR positivity. This protocol was then used to analyse milk samples from sheep of three different farms in Southern Italy, including real time PCR for DNA quantification and PCR-restriction fragment length polymorphism for genotyping. The pre-treatment protocol using ethylenediaminetetraacetic acid and Tris-HCl to remove casein gave the best results in the least amount of time compared to the others on spiked milk samples. One sample of 21 collected from sheep farms was positive on one-step PCR, real time PCR and resulted in a Type I genotype at one locus (SAG3. Milk usually contains a low number of tachyzoites and this could be a limiting factor for molecular identification. Our preliminary data has evaluated a rapid, cost-effective and sensitive protocol to treat milk before DNA extraction. The results of the present study also confirm the possibility of T. gondii transmission through consumption of raw milk and its unpasteurised derivatives.

EPA Metadata Style Guide Keywords and EPA Organization Names

Science.gov (United States)

The following keywords and EPA organization names listed below, along with EPA’s Metadata Style Guide, are intended to provide suggestions and guidance to assist with the standardization of metadata records.

Testing protocols for evaluating monolithic waste forms containing mixed wastes

International Nuclear Information System (INIS)

Gilliam, T.M.; Sams, T.L.; Pitt, W.W.

1986-01-01

Test protocols have been presented which can be used as a guide in cement-based grout formulation development studies. Based on experience at ORNL, these six tests are generally sufficient to develop a grout product which will meet all applicable DOE, NRC, and EPA performance criteria. As such, these tests can be used to minimize the time required to tailor a grout to be compatible with both the waste stream and the process disposal scenario. 9 refs

GAC-EPA

CERN Multimedia

GAC-EPA

2016-01-01

Le GAC organise chaque mois des permanences avec entretiens individuels. La prochaine permanence se tiendra le : Mardi 1er novembre de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel La permanence suivante aura lieu le mardi 29 novembre 2016. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/ Formulaire de contact : http://gac-epa.org/Organization/ContactForm/ContactForm-fr.php  

GAC-EPA

CERN Multimedia

GAC-EPA

2017-01-01

Le GAC organise des permanences avec entretiens individuels qui se tiennent le dernier mardi de chaque mois, sauf en juin, juillet et décembre. La prochaine permanence se tiendra le : Mardi 28 novembre de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/ Formulaire de contact : http://gac-epa.org/Organization/ContactForm/ContactForm-fr.php

GAC-EPA

CERN Multimedia

GAC-EPA

2017-01-01

Le GAC organise des permanences avec entretiens individuels qui se tiennent le dernier mardi de chaque mois, sauf en juin, juillet et décembre. La prochaine permanence se tiendra le : Mardi 30 mai de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/ Formulaire de contact : http://gac-epa.org/Organization/ContactForm/ContactForm-fr.php

EPA Region 1 Environmentally Sensitive Areas

Data.gov (United States)

U.S. Environmental Protection Agency — This coverage represents polygon equivalents of environmentally sensitive areas (ESA) in EPA Region I. ESAs were developed as part of an EPA headquarters initiative...

U.S. EPA Metadata Editor (EME)

Data.gov (United States)

U.S. Environmental Protection Agency — The EPA Metadata Editor (EME) allows users to create geospatial metadata that meets EPA's requirements. The tool has been developed as a desktop application that...

Quality-assurance procedures: Method 5G determination of particulate emissions from wood heaters from a dilution tunnel sampling location

Energy Technology Data Exchange (ETDEWEB)

Ward, T.E.; Hartman, M.W.; Olin, R.C.; Rives, G.D.

1989-06-01

Quality-assurance procedures are contained in this comprehensive document intended to be used as an aid for wood-heater manufacturers and testing laboratories in performing particulate matter sampling of wood heaters according to EPA protocol, Method 5G. These procedures may be used in research and development, and as an aid in auditing and certification testing. A detailed, step-by-step quality assurance guide is provided to aid in the procurement and assembly of testing apparatus, to clearly describe the procedures, and to facilitate data collection and reporting. Suggested data sheets are supplied that can be used as an aid for both recordkeeping and certification applications. Throughout the document, activity matrices are provided to serve as a summary reference. Checklists are also supplied that can be used by testing personnel. Finally, for the purposes of ensuring data quality, procedures are outlined for apparatus operation, maintenance, and traceability. These procedures combined with the detailed description of the sampling and analysis protocol will help ensure the accuracy and reliability of Method 5G emission-testing results.

EPA Facility Registry System (FRS): NCES

Science.gov (United States)

This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link to the National Center for Education Statistics (NCES). The primary federal database for collecting and analyzing data related to education in the United States and other Nations, NCES is located in the U.S. Department of Education, within the Institute of Education Sciences. FRS identifies and geospatially locates facilities, sites or places subject to environmental regulations or of environmental interest. Using vigorous verification and data management procedures, FRS integrates facility data from EPA00e2??s national program systems, other federal agencies, and State and tribal master facility records and provides EPA with a centrally managed, single source of comprehensive and authoritative information on facilities. This data set contains the subset of FRS integrated facilities that link to NCES school facilities once the NCES data has been integrated into the FRS database. Additional information on FRS is available at the EPA website http://www.epa.gov/enviro/html/fii/index.html.

2011 EPA Pesticide General Permit (PGP)

Data.gov (United States)

U.S. Environmental Protection Agency — The 2011 EPA Pesticide General Permit (PGP) covers discharges of biological pesticides, and chemical pesticides that leave a residue, in areas where EPA is the NPDES...

GAC-EPA

CERN Multimedia

GAC-EPA

2017-01-01

Le GAC organise des permanences avec entretiens individuels qui se tiennent le dernier mardi de chaque mois, sauf en juin, juillet et décembre. La prochaine permanence se tiendra le : Mardi 31 octobre de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel La permanence suivante aura lieu le mardi 28 novembre 2017. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/ Formulaire de contact : http://gac-epa.org/Organization/ContactForm/ContactForm-fr.php

GAC-EPA

CERN Multimedia

GAC-EPA

2017-01-01

Le GAC organise des permanences avec entretiens individuels qui se tiennent le dernier mardi de chaque mois, sauf en juin, juillet et décembre. La prochaine permanence se tiendra le : Mardi 29 août de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/ Formulaire de contact : http://gac-epa.org/Organization/ContactForm/ContactForm-fr.php  

Urine sample collection protocols for bioassay samples

Energy Technology Data Exchange (ETDEWEB)

MacLellan, J.A.; McFadden, K.M.

1992-11-01

In vitro radiobioassay analyses are used to measure the amount of radioactive material excreted by personnel exposed to the potential intake of radioactive material. The analytical results are then used with various metabolic models to estimate the amount of radioactive material in the subject`s body and the original intake of radioactive material. Proper application of these metabolic models requires knowledge of the excretion period. It is normal practice to design the bioassay program based on a 24-hour excretion sample. The Hanford bioassay program simulates a total 24-hour urine excretion sample with urine collection periods lasting from one-half hour before retiring to one-half hour after rising on two consecutive days. Urine passed during the specified periods is collected in three 1-L bottles. Because the daily excretion volume given in Publication 23 of the International Commission on Radiological Protection (ICRP 1975, p. 354) for Reference Man is 1.4 L, it was proposed to use only two 1-L bottles as a cost-saving measure. This raised the broader question of what should be the design capacity of a 24-hour urine sample kit.

Urine sample collection protocols for bioassay samples

Energy Technology Data Exchange (ETDEWEB)

MacLellan, J.A.; McFadden, K.M.

1992-11-01

In vitro radiobioassay analyses are used to measure the amount of radioactive material excreted by personnel exposed to the potential intake of radioactive material. The analytical results are then used with various metabolic models to estimate the amount of radioactive material in the subject's body and the original intake of radioactive material. Proper application of these metabolic models requires knowledge of the excretion period. It is normal practice to design the bioassay program based on a 24-hour excretion sample. The Hanford bioassay program simulates a total 24-hour urine excretion sample with urine collection periods lasting from one-half hour before retiring to one-half hour after rising on two consecutive days. Urine passed during the specified periods is collected in three 1-L bottles. Because the daily excretion volume given in Publication 23 of the International Commission on Radiological Protection (ICRP 1975, p. 354) for Reference Man is 1.4 L, it was proposed to use only two 1-L bottles as a cost-saving measure. This raised the broader question of what should be the design capacity of a 24-hour urine sample kit.

Characterizing Contamination and Assessing Exposure, Risk and Resilience

Science.gov (United States)

EPA supports its responders' ability to characterize site contamination by developing sampling protocols, sample preparation methods, and analytical methods for chemicals, biotoxins, microbial pathogens, and radiological agents.

Purchasing Supplies, Equipment and Services Under EPA Grants

Science.gov (United States)

EPA developed this guidance to help ensure you meet EPA requirements when making such necessary purchases. With very few exceptions, you must follow a competitive process when you use EPA grant funds to acquire equipment and professional services.

Soil Sampling Operating Procedure

Science.gov (United States)

EPA Region 4 Science and Ecosystem Support Division (SESD) document that describes general and specific procedures, methods, and considerations when collecting soil samples for field screening or laboratory analysis.

Notification: EPA's Preparedness and Response Efforts to the 2017 Hurricanes in EPA Regions 2, 4 and 6

Science.gov (United States)

Project #OPE-FY18-0005, December 13, 2017. The EPA OIG plans to begin preliminary research on the EPA’s preparedness and response efforts to the 2017 hurricanes that impacted EPA Regions 2, 4 and 6.

EPA Regulation of Bed Bug Pesticides

Science.gov (United States)

All pesticides must be registered by EPA before being sold and used in the U.S., other than those that rely on a limited set of active ingredients (so-called minimum risk pesticides). EPA reviews for safety and effectiveness.

EPA Office Points, Tutuila AS, 2009, US EPA Region 9

Data.gov (United States)

U.S. Environmental Protection Agency — The EPA office location in Tutila Island in American Samoa. American Samoa is an unincorporated and unorganized territory of the United States, and administered by...

GAC-EPA

CERN Multimedia

GAC-EPA

2017-01-01

Le GAC organise des permanences avec entretiens individuels qui se tiennent le dernier mardi de chaque mois, sauf en juin, juillet et décembre. La prochaine permanence se tiendra le : Mardi 26 septembre de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 31 octobre et 28 novembre 2017. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/ Formulaire de contact : http://gac-epa.org/Organization/ContactForm/ContactForm-fr.php

EPA Envirofacts API

Data.gov (United States)

U.S. Environmental Protection Agency — Envirofacts integrates information from a variety of EPA's environmental databases. Each of these databases contains information about facilities that are required...

Bioinspired Security Analysis of Wireless Protocols

DEFF Research Database (Denmark)

Petrocchi, Marinella; Spognardi, Angelo; Santi, Paolo

2016-01-01

work, this paper investigates feasibility of adopting fraglets as model for specifying security protocols and analysing their properties. In particular, we give concrete sample analyses over a secure RFID protocol, showing evolution of the protocol run as chemical dynamics and simulating an adversary...

EPA Recovery Mapper

Data.gov (United States)

U.S. Environmental Protection Agency — The EPA Recovery Mapper is an Internet interactive mapping application that allows users to discover information about every American Recovery and Reinvestment Act...

EPA requirements for the uranium fuel cycle

International Nuclear Information System (INIS)

Dunster, H.J.

1975-01-01

The draft Environmental Statement issued by the Environmental Protection Agency (EPA) in the United States in preparation for Proposed Rulemaking Action concerning 'Environmental radiation protection requirements for normal operations of activities in the uranium fuel cycle' is summarized and discussed. The standards proposed by the EPA limit the annual dose equivalents to any member of the public, and also the releases of radionuclides to the 'general environment' for each gigawatt year of electrical energy produced. These standards were based on cost effectiveness arguements and levels and correspond to the ICRP recommendation to keep all exposures as low as reasonably achievable, economic and social factors being taken into account. They should be clearly distinguished from dose limits, although the EPA does not make this at all clear. The EPA seems to have shown an unexpected lack of understanding of the recommendations of ICRP Publication 9 (1965) and an apparent unawareness of ICRP Publication 22 (1973), and has therefore wrongly presented the new standards as a significant change in policy. The EPA has reviewed the information on the likely level of dose equivalents to members of the public and the likely cost reductions, thereby quantifying existing principles as applied to the fuel cycle as a whole. The EPA has stated that its proposals could be achieved as a cost in the region of Pound100,000 per death (or major genetic defect). It is pointed out that the EPA's use of the term 'waste' to exclude liquid and gaseous effluents may cause confusion. (U.K.)

Hispanos en la EPA: Grace Robiou

Science.gov (United States)

La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente.

Hispanos en la EPA: Fabiola Estrada

Science.gov (United States)

La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente.

Hispanos en la EPA: Matthew Tejada

Science.gov (United States)

La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente.

Hispanos en la EPA: Joel Corona

Science.gov (United States)

La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente.

GAC-EPA

CERN Multimedia

GAC-EPA

2017-01-01

Le GAC organise des permanences avec entretiens individuels qui se tiennent le dernier mardi de chaque mois, sauf en juin, juillet et décembre. La prochaine permanence se tiendra le : Mardi 25 avril de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 30 mai, 29 août, 26 septembre, 31 octobre et 28 novembre 2017. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. Formulaire de contact : http://gac-epa.org/Organization/ContactForm/ContactForm-fr.php

GAC-EPA

CERN Multimedia

GAC-EPA

2017-01-01

Le GAC organise des permanences avec entretiens individuels qui se tiennent le dernier mardi de chaque mois, sauf en juin, juillet et décembre. La prochaine permanence se tiendra le : Mardi 28 février de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 28 mars, 25 avril, 30 mai, 29 août, 26 septembre, 31 octobre et 28 novembre 2017. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. Formulaire de contact : http://gac-epa.org/Organization/ContactForm/ContactForm-fr.php

GAC-EPA

CERN Multimedia

GAC-EPA

2017-01-01

Le GAC organise des permanences avec entretiens individuels qui se tiennent le dernier mardi de chaque mois, sauf en juin, juillet et décembre. La prochaine permanence se tiendra le : Mardi 28 mars de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 25 avril, 30 mai, 29 août, 26 septembre, 31 octobre et 28 novembre 2017. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. Formulaire de contact : http://gac-epa.org/Organization/ContactForm/ContactForm-fr.php

GAC-EPA

CERN Multimedia

GAC-EPA

2017-01-01

Le GAC organise des permanences avec entretiens individuels qui se tiennent le dernier mardi de chaque mois, sauf en juin, juillet et décembre. La prochaine permanence se tiendra le : Mardi 30 mai de 13 h 30 à 16 h 00 Salle de réunion de l’Association du personnel Les permanences suivantes auront lieu les mardis 29 août, 26 septembre, 31 octobre et 28 novembre 2017. Les permanences du Groupement des Anciens sont ouvertes aux bénéficiaires de la Caisse de pensions (y compris les conjoints survivants) et à tous ceux qui approchent de la retraite. Nous invitons vivement ces derniers à s’associer à notre groupement en se procurant, auprès de l’Association du personnel, les documents nécessaires. Informations : http://gac-epa.org/. Formulaire de contact : http://gac-epa.org/Organization/ContactForm/ContactForm-fr.php

EPA Linked Open Data: Substance Registry Service

Data.gov (United States)

U.S. Environmental Protection Agency — Substance Registry Services (SRS) is the Environmental Protection Agency's (EPA) central system for information about substances that are tracked or regulated by EPA...

Low-level and narm radioactive wastes. Model documentation: accounting model for PRESTO-EPA-POP, PRESTO-EPA-DEEP, and PRESTO-EPA-BRC. Methodology and users manual. Final report

International Nuclear Information System (INIS)

Rogers, V.; Hung, C.

1987-12-01

The accounting model was used as a utility model for assessing the cumulative health effects to the general population residing in the downstream regional water basin as a result of the disposal of LLW when a unit response analysis method is used. The utility model is specifically designed to assess the cumulative population health effects in conjunction with the PRESTO-EPA-POP, PRESTO-EPA-BRC, or PRESTO-EPA-DEEP model simply for the purpose of reducing the cost of analysis. Therefore, the assessment of the cumulative population health effects may also be conducted with one of the above appropriate models without the aid of this accounting model

EPA User Personas

Science.gov (United States)

Learn how EPA's three web user personas (Information Consumer, Information Intermediary, and Information Interpreter) can help you identify appropriate top audiences and top tasks for a topic or web area.

EPA Nanorelease Dataset

Data.gov (United States)

U.S. Environmental Protection Agency — EPA Nanorelease Dataset. This dataset is associated with the following publication: Wohlleben, W., C. Kingston, J. Carter, E. Sahle-Demessie, S. Vazquez-Campos, B....

78 FR 63093 - Approval and Promulgation of Air Quality Implementation Plans; Indiana

Science.gov (United States)

2013-10-23

... section into the Indiana SIP. 326 IAC 3-6-2 Source Sampling Protocols This section requires the owner or... Indiana SIP. 326 IAC 3-7-4 Fuel Oil Sampling; Analysis Methods This section specifies the test protocol... monitoring rules to EPA as a revision to its state implementation plan (SIP). The monitoring rules will be...

40 CFR 141.702 - Sampling schedules.

Science.gov (United States)

2010-07-01

... serving at least 10,000 people must submit their sampling schedule for the initial round of source water... submitting the sampling schedule that EPA approves. (3) Systems serving fewer than 10,000 people must submit... analytical result for a scheduled sampling date due to equipment failure, loss of or damage to the sample...

Evaluation of a lateral flow-based technology card for blood typing using a simplified protocol in a model of extreme blood sampling conditions.

Science.gov (United States)

Clavier, Benoît; Pouget, Thomas; Sailliol, Anne

2018-02-01

Life-threatening situations requiring blood transfusion under extreme conditions or in remote and austere locations, such as the battlefield or in traffic accidents, would benefit from reliable blood typing practices that are easily understood by a nonscientist or nonlaboratory technician and provide quick results. A simplified protocol was developed for the lateral flow-based device MDmulticard ABO-D-Rh subgroups-K. Its performance was compared to a reference method (PK7300, Beckman Coulter) in native blood samples from donors. The method was tested on blood samples stressed in vitro as a model of hemorrhage cases (through hemodilution using physiologic serum) and dehydration (through hemoconcentration by removing an aliquot of plasma after centrifugation), respectively. A total of 146 tests were performed on 52 samples; 126 in the hemodilution group (42 for each native, diluted 1/2, and diluted 1/4 samples) and 20 in the hemoconcentration group (10 for each native and 10% concentrated samples). Hematocrit in the tested samples ranged from 9.8% to 57.6% while hemoglobin levels ranged from 3.2 to 20.1 g/dL. The phenotype profile detected with the MDmulticard using the simplified protocol resulted in 22 A, seven B, 20 O, and three AB, of which nine were D- and five were Kell positive. No discrepancies were found with respect to the results obtained with the reference method. The simplified protocol for MDmulticard use could be considered a reliable method for blood typing in extreme environment or emergency situations, worsened by red blood cell dilution or concentration. © 2017 AABB.

Perfiles de hispanos en la EPA

Science.gov (United States)

La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente de todos.

EPA's role in uranium mining and milling

International Nuclear Information System (INIS)

Smith, P.B.

1980-01-01

EPA's role and actions in regulating uranium mining and milling are reviewed and updated. Special emphasis is given to EPA's current activities under the Uranium Mill Tailings Radiation Control Act of 1978

Pollution prevention initiatives at US EPA: 'Green Lights'

International Nuclear Information System (INIS)

Lawson, J.; Kwartin, R.

1991-01-01

US EPA is initiating a pollution prevention approach to supplement its historic command-control, regulatory approach to environmental protection. EPA believes polllution prevention, where applicable and possible, represents a quicker, less expensive and even profitable strategy for environmental protection. Most clearly, energy-efficiency provides an opportunity to prevent significant amounts of pollution related to the inefficeint generation and use of electricity. EPA's first energy productivity and pollution prevention program is Green Lights. Beyond its own merits, Green Lights will also provide important experience to EPA as it develops its Green Machines program to accelerate the market for efficient appliances and equipment

Privacy Act System of Records: EPA Telecommunications Detail Records, EPA-32

Science.gov (United States)

Learn more about the EPA Telecommunications Detail Records System, including who is covered in the system, the purpose of data collection, routine uses for the system's records, and other security procedures.

40 CFR 73.52 - EPA recordation.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 16 2010-07-01 2010-07-01 false EPA recordation. 73.52 Section 73.52... ALLOWANCE SYSTEM Allowance Transfers § 73.52 EPA recordation. (a) General recordation. Except as provided in...) following receipt of an allowance transfer request pursuant to § 73.50, by moving each allowance from the...

Soil Gas Sampling Operating Procedure

Science.gov (United States)

EPA Region 4 Science and Ecosystem Support Division (SESD) document that describes general and specific procedures, methods, and considerations when collecting soil gas samples for field screening or laboratory analysis.

EPA Region 1 Sole Source Aquifers

Science.gov (United States)

This coverage contains boundaries of EPA-approved sole source aquifers. Sole source aquifers are defined as an aquifer designated as the sole or principal source of drinking water for a given aquifer service area; that is, an aquifer which is needed to supply 50% or more of the drinking water for the area and for which there are no reasonable alternative sources should the aquifer become contaminated.The aquifers were defined by a EPA hydrogeologist. Aquifer boundaries were then drafted by EPA onto 1:24000 USGS quadrangles. For the coastal sole source aquifers the shoreline as it appeared on the quadrangle was used as a boundary. Delineated boundaries were then digitized into ARC/INFO.

Meet EPA Engineer Shawn Ryan, Ph.D.

Science.gov (United States)

Shawn Ryan, Ph.D. is a chemical engineer at EPA's National Homeland Security Research Center. He has worked at EPA for 12 years, nine of which have been devoted to leading research to support decontamination and consequence management.

EPA Region 1 - Valley Depth in Meters

Science.gov (United States)

Raster of the Depth in meters of EPA-delimited Valleys in Region 1.Valleys (areas that are lower than their neighbors) were extracted from a Digital Elevation Model (USGS, 30m) by finding the local average elevation, subtracting the actual elevation from the average, and selecting areas where the actual elevation was below the average. The landscape was sampled at seven scales (circles of 1, 2, 4, 7, 11, 16, and 22 km radius) to take into account the diversity of valley shapes and sizes. Areas selected in at least four scales were designated as valleys.

Meet EPA Ecologist Paul Mayer, Ph.D.

Science.gov (United States)

EPA ecologist Paul Mayer, Ph.D. works in EPA's Groundwater and Ecosystem Restoration division where he studies riparian zones (the area along rivers and streams where the habitats are influenced by both the land and water) and stream restoration

US EPA Regional Masks Web Service, US, 2015, US EPA, SEGS

Data.gov (United States)

U.S. Environmental Protection Agency — This web service contains the following map layers: masks and labels for EPA regions 1 through 10. Mask layers are drawn at all scales. Label layers draw at scales...

Proposed Changes to EPA's Transuranic Waste Characterization Approval Process

International Nuclear Information System (INIS)

Joglekar, R.D.; Feltcorn, E.M.; Ortiz, A.M.

2003-01-01

This paper describes the changes to the waste characterization (WC) approval process proposed in August 2002 by the U.S. Environmental Protection Agency (EPA or the Agency or we). EPA regulates the disposal of transuranic (TRU) waste at the Waste Isolation Pilot Plant (WIPP) repository in Carlsbad, New Mexico. EPA regulations require that waste generator/storage sites seek EPA approval of WC processes used to characterize TRU waste destined for disposal at WIPP. The regulations also require that EPA verify, through site inspections, characterization of each waste stream or group of waste streams proposed for disposal at the WIPP. As part of verification, the Agency inspects equipment, procedures, and interviews personnel to determine if the processes used by a site can adequately characterize the waste in order to meet the waste acceptance criteria for WIPP. The paper discusses EPA's mandate, current regulations, inspection experience, and proposed changes. We expect that th e proposed changes will provide equivalent or improved oversight. Also, they would give EPA greater flexibility in scheduling and conducting inspections, and should clarify the regulatory process of inspections for both Department of Energy (DOE) and the public

Effect of eicosapentaenoic acid/docosahexaenoic acid on coronary high-intensity plaques detected with non-contrast T1-weighted imaging (the AQUAMARINE EPA/DHA study): study protocol for a randomized controlled trial.

Science.gov (United States)

Nakao, Kazuhiro; Noguchi, Teruo; Asaumi, Yasuhide; Morita, Yoshiaki; Kanaya, Tomoaki; Fujino, Masashi; Hosoda, Hayato; Yoneda, Shuichi; Kawakami, Shoji; Nagai, Toshiyuki; Nishihira, Kensaku; Nakashima, Takahiro; Kumasaka, Reon; Arakawa, Tetsuo; Otsuka, Fumiyuki; Nakanishi, Michio; Kataoka, Yu; Tahara, Yoshio; Goto, Yoichi; Yamamoto, Haruko; Hamasaki, Toshimitsu; Yasuda, Satoshi

2018-01-08

Despite the success of HMG-CoA reductase inhibitor (statin) therapy in reducing atherosclerotic cardiovascular events, a residual risk for cardiovascular events in patients with coronary artery disease (CAD) remains. Long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are promising anti-atherosclerosis agents that might reduce the residual CAD risk. Non-contrast T1-weighted imaging (T1WI) with cardiac magnetic resonance (CMR) less invasively identifies high-risk coronary plaques as high-intensity signals. These high-intensity plaques (HIPs) are quantitatively assessed using the plaque-to-myocardium signal intensity ratio (PMR). Our goal is to assess the effect of EPA/DHA on coronary HIPs detected with T1WI in patients with CAD on statin treatment. This prospective, controlled, randomized, open-label study examines the effect of 12 months of EPA/DHA therapy and statin treatment on PMR of HIPs detected with CMR and computed tomography angiography (CTA) in patients with CAD. The primary endpoint is the change in PMR after EPA/DHA treatment. Secondary endpoints include changes in Hounsfield units, plaque volume, vessel area, and plaque area measured using CTA. Subjects are randomly assigned to either of three groups: the 2 g/day EPA/DHA group, the 4 g/day EPA/DHA group, or the no-treatment group. This trial will help assess whether EPA/DHA has an anti-atherosclerotic effect using PMR of HIPs detected by CMR. The trial outcomes will provide novel insights into the effect of EPA/DHA on high-risk coronary plaques and may provide new strategies for lowering the residual risk in patients with CAD on statin therapy. The University Hospital Medical Information Network (UMIN) Clinical Trials Registry, ID: UMIN000015316 . Registered on 2 October 2014.

Towards the production of EPA: a developing country perspective

CSIR Research Space (South Africa)

Maharajh, Dheepak M

2015-03-01

Full Text Available are focussed towards increased production of high purity EPA. This provides a window of opportunity for a supply of competing products that are rich in algal EPA. Recently the CSIR developed a technology for the production of EPA by an indigenous algal isolate...

Pierre Gy's sampling theory and sampling practice heterogeneity, sampling correctness, and statistical process control

CERN Document Server

Pitard, Francis F

1993-01-01

Pierre Gy's Sampling Theory and Sampling Practice, Second Edition is a concise, step-by-step guide for process variability management and methods. Updated and expanded, this new edition provides a comprehensive study of heterogeneity, covering the basic principles of sampling theory and its various applications. It presents many practical examples to allow readers to select appropriate sampling protocols and assess the validity of sampling protocols from others. The variability of dynamic process streams using variography is discussed to help bridge sampling theory with statistical process control. Many descriptions of good sampling devices, as well as descriptions of poor ones, are featured to educate readers on what to look for when purchasing sampling systems. The book uses its accessible, tutorial style to focus on professional selection and use of methods. The book will be a valuable guide for mineral processing engineers; metallurgists; geologists; miners; chemists; environmental scientists; and practit...

Investigations of the post-IR IRSL protocol applied to single K-feldspar grains from fluvial sediment samples

International Nuclear Information System (INIS)

Nian, Xiaomei; Bailey, Richard M.; Zhou, Liping

2012-01-01

The post-IR IRSL protocol with single K-feldspar grains was applied to three samples taken from a fluvial sedimentary sequence at the archaeological site of the Dali Man, Shaanxi Province, China. K-feldspar coarse grains were extracted for measurement. Approximately 30–40% of the grains were sufficiently bright to measure, and after application of rejection criteria based on signal strength, recuperation, recycling ratio and saturation dose, ∼10–15% of the grains were used for D e calculation. The relationship of signal decay rate and form of D e (t) with the recovery dose were investigated. The dose recovery ratios of the samples after initial bleaching with the four different light sources were within uncertainties of unity. No anomalous fading was observed. The over-dispersion of the recovered dose and D e values were similar, suggesting neither incomplete resetting of the post-IR IRSL signals nor spatially heterogeneous dose rates significantly affected the natural dose estimates. The values of D e obtained with the single K-feldspar grain post-IR IRSL protocol were in the range ∼400–490 Gy. Combining all of the measured single-grain signals for each of the individual samples (into a ‘synthetic single aliquot’) increased the D e estimates to the range ∼700–900 Gy, suggesting that the grains screened-out by the rejection criteria may have the potential to cause palaeodose over-estimation, although this finding requires a more extensive investigation. Thermally transferred signals were found in the single K-feldspar grains post-IR IRSL protocol, and the proportion of thermally transferred signal to test-dose OSL signal (stimulation at 290 °C) from the natural dose was higher than from regenerative doses, and the proportion was grain- and dose-dependent. As such, TT-post-IR IRSL signals at 290 °C have the potential to cause dose underestimation, although this may be reduced by using larger test-dose irradiations. Our study demonstrates

Cleanup protocols when encountering thorium-230 at U.S. DOE Uranium Mill Tailings Remedial Action (UMTRA) Project sites

International Nuclear Information System (INIS)

Miller, M.L.; Hylko, J.M.; Cornish, R.E.

1995-01-01

The passage of the Uranium Mill Tailings Radiation Control Act (UMTRCA) of 1978, established the regulatory framework, under which the US EPA charged with developing standards for the cleanup and disposal of tailings at 24 designated inactive uranium processing sites located in 10 states. 40 CFR 192.12 requires that the concentration of Ra-226 in land averaged over any area of 100 square meters shall not exceed the background level by more than 5 pCi/g, averaged over the first 15 cm of soil below the surface, 15 pCi/g, averaged over 15-cm-thick layers of soils more than 15 cm below the surface. However, Th-230 is not specifically addressed by the EPA in 40 CFR 192.12, which naturally decays with a half-life of 77,000 years to form Ra-226. Consequently, the cleanup of the initial Ra-226 contamination according to the standards will not necessarily mitigate against the eventual ingrowth of residual Ra-226 with time, due to the radioactive decay of residual Th-230. Therefore, to direct the excavation of residual Th-230, four generic protocols are being used at Uranium Mill Tailings Remedial Action (UMTRA) Project sites, as follows: Determining the allowable remaining concentration of Th-230 in surface and subsurface soils; Encountering Th-230 contamination in the unsaturated subsurface soil; Encountering Th-230 contamination in the saturated zone; and Verification sampling. The four generic protocols, developed in conjunction with the supplemental standards provision, ensure protection of the general public by reducing exposures to levels that are As Low As Reasonably Achievable, while considering practical measures necessary to excavate Th-230 under conditions encountered at the UMTRA Project site

EPA Facility Registry Service (FRS): Wastewater Treatment Plants

Data.gov (United States)

U.S. Environmental Protection Agency — This GIS dataset contains data on wastewater treatment plants, based on EPA's Facility Registry Service (FRS), EPA's Integrated Compliance Information System (ICIS)...

EPA SCIENCE FORUM - EPA'S TOXICOGENOMICS PARTNERSHIPS ACROSS GOVERNMENT, ACADEMIA AND INDUSTRY

Science.gov (United States)

Over the past decade genomics, proteomics and metabonomics technologies have transformed the science of toxicology, and concurrent advances in computing and informatics have provided management and analysis solutions for this onslaught of toxicogenomic data. EPA has been actively...

Transforming an EPA QA/R-2 quality management plan into an ISO 9002 quality management system.

Science.gov (United States)

Kell, R A; Hedin, C M; Kassakhian, G H; Reynolds, E S

2001-01-01

The Environmental Protection Agency's (EPA) Office of Emergency and Remedial Response (OERR) requires environmental data of known quality to support Superfund hazardous waste site projects. The Quality Assurance Technical Support (QATS) Program is operated by Shaw Environmental and Infrastructure, Inc. to provide EPA's Analytical Operations Center (AOC) with performance evaluation samples, reference materials, on-site laboratory auditing capabilities, data audits (including electronic media data audits), methods development, and other support services. The new QATS contract awarded in November 2000 required that the QATS Program become ISO 9000 certified. In a first for an EPA contractor, the QATS staff and management successfully transformed EPA's QA/R-2 type Quality Management Plan into a Quality Management System (QMS) that complies with the requirements of the internationally recognized ISO 9002 standard and achieved certification in the United States, Canada, and throughout Europe. The presentation describes how quality system elements of ISO 9002 were implemented on an already existing quality system. The psychological and organizational challenges of the culture change in QATS' day-to-day operations will be discussed for the benefit of other ISO 9000 aspirants.

Network protocols and sockets

OpenAIRE

BALEJ, Marek

2010-01-01

My work will deal with network protocols and sockets and their use in programming language C#. It will therefore deal programming network applications on the platform .NET from Microsoft and instruments, which C# provides to us. There will describe the tools and methods for programming network applications, and shows a description and sample applications that work with sockets and application protocols.

EPA Scientific Knowledge Management Assessment and Needs

Science.gov (United States)

A series of activities have been conducted by a core group of EPA scientists from across the Agency. The activities were initiated in 2012 and the focus was to increase the reuse and interoperability of science software at EPA. The need for increased reuse and interoperability ...

Hispanos en la EPA: Evelyn Rivera-Ocasio

Science.gov (United States)

La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente.

Hispanos en la EPA: Nadtya Y. Hong

Science.gov (United States)

La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente.

Privacy Act System of Records: EPA Personnel Emergency Contact Files, EPA-44

Science.gov (United States)

Learn about the EPA Personnel Emergency Contact Files System, including including who is covered in the system, the purpose of data collection, routine uses for the system's records, and other security procedure.

EPA's Final Financial Assistance Conflict of Interest Policy

Science.gov (United States)

EPA has established the following revised interim policy governing disclosure of actual and potential conflicts of interest (COI Policy) by applicants for, and recipients of, federal financial assistance awards from EPA.

EPA Region 1 Coast Guard Jurisdictional Boundary - Polygons

Data.gov (United States)

U.S. Environmental Protection Agency — Jurisdictional boundary between EPA and Coast Guard for EPA Region I. Created from 1:100000 USGS DLGs with greater detail drawn from 1:24000 commercial street data...

EPA Region 1 Coast Guard Jurisdictional Boundary - Arcs

Data.gov (United States)

U.S. Environmental Protection Agency — Jurisdictional boundary between EPA and Coast Guard for EPA Region I. Created from 1:100000 USGS DLGs with greater detail drawn from 1:24000 commercial street data...

Evaluation protocol for amusia: Portuguese sample.

Science.gov (United States)

Peixoto, Maria Conceição; Martins, Jorge; Teixeira, Pedro; Alves, Marisa; Bastos, José; Ribeiro, Carlos

2012-12-01

Amusia is a disorder that affects the processing of music. Part of this processing happens in the primary auditory cortex. The study of this condition allows us to evaluate the central auditory pathways. To explore the diagnostic evaluation tests of amusia. The authors propose an evaluation protocol for patients with suspected amusia (after brain injury or complaints of poor musical perception), in parallel with the assessment of central auditory processing, already implemented in the department. The Montreal Evaluation of Battery of amusia was the basis for the selection of the tests. From this comprehensive battery of tests we selected some of the musical examples to evaluate different musical aspects, including memory and perception of music, ability concerning musical recognition and discrimination. In terms of memory there is a test for assessing delayed memory, adapted to the Portuguese culture. Prospective study. Although still experimental, with the possibility of adjustments in the assessment, we believe that this assessment, combined with the study of central auditory processing, will allow us to understand some central lesions, congenital or acquired hearing perception limitations.

A two-hypothesis approach to establishing a life detection/biohazard protocol for planetary samples

Science.gov (United States)

Conley, Catharine; Steele, Andrew

2016-07-01

The COSPAR policy on performing a biohazard assessment on samples brought from Mars to Earth is framed in the context of a concern for false-positive results. However, as noted during the 2012 Workshop for Life Detection in Samples from Mars (ref. Kminek et al., 2014), a more significant concern for planetary samples brought to Earth is false-negative results, because an undetected biohazard could increase risk to the Earth. This is the reason that stringent contamination control must be a high priority for all Category V Restricted Earth Return missions. A useful conceptual framework for addressing these concerns involves two complementary 'null' hypotheses: testing both of them, together, would allow statistical and community confidence to be developed regarding one or the other conclusion. As noted above, false negatives are of primary concern for safety of the Earth, so the 'Earth Safety null hypothesis' -- that must be disproved to assure low risk to the Earth from samples introduced by Category V Restricted Earth Return missions -- is 'There is native life in these samples.' False positives are of primary concern for Astrobiology, so the 'Astrobiology null hypothesis' -- that must be disproved in order to demonstrate the existence of extraterrestrial life is 'There is no life in these samples.' The presence of Earth contamination would render both of these hypotheses more difficult to disprove. Both these hypotheses can be tested following a strict science protocol; analyse, interprete, test the hypotheses and repeat. The science measurements undertaken are then done in an iterative fashion that responds to discovery with both hypotheses testable from interpretation of the scientific data. This is a robust, community involved activity that ensures maximum science return with minimal sample use.

Career paths through the U.S. EPA

Science.gov (United States)

In a recent survey of employee satisfaction, the U.S. EPA was ranked 6th of the 30 large federal agencies that were surveyed (http://data.bestplacestowork.org). I have been working as a post doc at the EPA since receiving my Ph.D. in ecological physiology from the University of ...

EPA Scientific Knowledge Management Assessment and ...

Science.gov (United States)

A series of activities have been conducted by a core group of EPA scientists from across the Agency. The activities were initiated in 2012 and the focus was to increase the reuse and interoperability of science software at EPA. The need for increased reuse and interoperability is linked to the increased complexity of environmental assessments in the 21st century. This complexity is manifest in the form of problems that require integrated multi-disciplinary solutions. To enable the means to develop these solutions (i.e., science software systems) it is necessary to integrate software developed by disparate groups representing a variety of science domains. Thus, reuse and interoperability becomes imperative. This report briefly describes the chronology of activities conducted by the group of scientists to provide context for the primary purpose of this report, that is, to describe the proceedings and outcomes of the latest activity, a workshop entitled “Workshop on Advancing US EPA integration of environmental and information sciences”. The EPA has been lagging in digital maturity relative to the private sector and even other government agencies. This report helps begin the process of improving the agency’s use of digital technologies, especially in the areas of efficiency and transparency. This report contributes to SHC 1.61.2.

KCBX Petroleum Coke Storage Pile Sampling Logbook and Photos

Science.gov (United States)

This documentation of KCBX's petcoke sampling locations and methods demonstrates adherence to the pet coke sampling plan previously submitted and approved by EPA, at both North Terminal and South Terminal stockpiles.

Development and Evaluation of EPA Method 1615 for Detection of Enterovirus and Norovirus in Water

Science.gov (United States)

The U.S. EPA developed a sample concentration and preparation assay in conjunction with the Total Culturable Virus Assay for concentrating and measuring culturable viruses in source and drinking waters as part of the Information Collection Rule promulgated in 1996. In an effort...

EPA'S strategy to reduce risk of radon

International Nuclear Information System (INIS)

Page, S.

1993-01-01

The Indoor Radon Abatement Act of 1988 (IRAA) directed EPA to undertake a variety of activities to address the growing public concern over dangers posed by exposure to indoor radon. Among other requirements, the law directed the Agency to study radon levels, evaluate mitigation methods, establish proficiency programs, assist states with program development, develop training centers, and provide public information. EPA has developed and implemented programs to address each of the key provisions of this statute. This paper presents EPA's broad national strategy to reduce radon risks. It combines and reinforces EPA's basic foundation, including its guiding policies and cooperative partnerships, with an overall management approach and focus for the future. The paper starts with an overview that introduces the strategy's four key elements: underlying policies and scientific principles, a decentralized system of states and other partners for targeting the public, multiple strategies for achieving radon risk reduction, and a strong focus on five key program priorities. This paper then discusses each of these elements in more detail and describes how they interact to guide future efforts and directions of the Agency

Nonattainment Areas in Louisiana, Geographic NAD83, EPA (2006) [Nonattainment_LA_EPA_2006

Data.gov (United States)

Louisiana Geographic Information Center — EPA Region 6 NonAttainment Areas in Louisiana, current as of May 2006. This shapefile contains parish boundaries and attributes that determine whether the parishes...

40 CFR 1045.345 - What production-line testing records must I send to EPA?

Science.gov (United States)

2010-07-01

... must I send to EPA? 1045.345 Section 1045.345 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... nonroutine adjustment, modification, repair, preparation, maintenance, or test for the test engine if you did... sample-size calculation required in § 1045.310 for each engine family. (9) Report on each failed engine...

40 CFR 1048.345 - What production-line testing records must I send to EPA?

Science.gov (United States)

2010-07-01

... must I send to EPA? 1048.345 Section 1048.345 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... nonroutine adjustment, modification, repair, preparation, maintenance, or test for the test engine if you did... the sample-size calculation required in § 1048.310 for each engine family. (9) Report on each failed...

Regulation of higher-activity NARM wastes by EPA

International Nuclear Information System (INIS)

Bandrowski, M.S.

1988-01-01

The US Environmental Protection Agency (EPA) is currently developing standards for the disposal of low-level radioactive waste (LLW). As part of this Standard, EPA is including regulations for the disposal of naturally occurring and accelerator-produced radioactive material (NARM) wastes not covered under the Atomic Energy Act (AEA). The regulations will cover only higher-activity NARM wastes, defined as NARM waste with specific activity exceeding two nanocuries per gram. The proposed regulations will specify that NARM wastes exceeding the above limits, except for specific exempted items, must be disposed of in regulated radioactive waste disposal facilities. The proposed EPA regulations for NARM wastes will be discussed, as well as the costs and benefits of the regulation, how it will be implemented by EPA, and the rationale for covering only higher-activity NARM wastes exceeding two nanocuries per gram

UMTRA project water sampling and analysis plan, Maybell, Colorado

International Nuclear Information System (INIS)

1994-06-01

This water sampling and analysis plan (WSAP) describes planned water sampling activities and provides the regulatory and technical basis for ground water sampling in 1994 at the US Department of Energy's (DOE) Uranium Mill Tailings Remedial Action (UMTRA) Project site in Maybell, Colorado. The WSAP identifies and justifies sampling locations, analytical parameters, and sampling frequencies at the site. The ground water data will be used for site characterization and risk assessment. The regulatory basis for the ground water and surface water monitoring activities is derived from the EPA regulations in 40 CFR Part 192 (1993) and the proposed EPA standards of 1987 (52 FR 36000). Sampling procedures are guided by the UMTRA Project standard operating procedures (SOP) (JEG, n.d.), the Technical Approach Document (TAD) (DOE, 1989), and the most effective technical approach for the site. This WSAP also includes a summary and the results of water sampling activities from 1989 through 1992 (no sampling was performed in 1993)

EPA's Efforts in Eurasia

Science.gov (United States)

EPA has been collaborating with Russia, Central Asia, the Caucasus and Ukraine for over 20 years and continues to work with their governments and non-governmental organizations on environment, science and technology issues.

Effects of a 12-week high-α-linolenic acid intervention on EPA and DHA concentrations in red blood cells and plasma oxylipin pattern in subjects with a low EPA and DHA status.

Science.gov (United States)

Greupner, Theresa; Kutzner, Laura; Nolte, Fabian; Strangmann, Alena; Kohrs, Heike; Hahn, Andreas; Schebb, Nils Helge; Schuchardt, Jan Philipp

2018-03-01

The essential omega-3 fatty acid alpha-linolenic acid (ALA, 18:3n3) can be converted into EPA and DHA. The aim of the present study was to determine the effect of a high-ALA diet on EPA and DHA levels in red blood cells (RBCs) and their oxylipins in the plasma of subjects with a low EPA and DHA status. Fatty acid concentrations [μg mL -1 ] and relative amounts [% of total fatty acids] in the RBCs of 19 healthy men (mean age 26.4 ± 4.6 years) were analyzed by means of GC-FID. Free plasma oxylipin concentrations were determined by LC-MS based targeted metabolomics. Samples were collected and analyzed at baseline (week 0) and after 1 (week 1), 3 (week 3), 6 (week 6), and 12 (week 12) weeks of high dietary ALA intake (14.0 ± 0.45 g day -1 ). ALA concentrations significantly (p DHA concentrations unexpectedly decreased from 41.0 ± 1.93 (week 0) to 37.0 ± 1.32 (week 1), 36.1 ± 1.37 (week 3), 35.1 ± 1.06 (p = 0.010, week 6), and 30.4 ± 1.09 (p DHA amounts were unchanged during the intervention (week 0: 4.63 ± 0.19, week 1: 4.67 ± 0.16, week 3: 4.61 ± 0.13, week 6: 4.73 ± 0.15, week 12: 4.52 ± 0.11). ALA- and EPA-derived hydroxy- and dihydroxy-PUFA increased similarly to their PUFA precursors, although in the case of ALA-derived oxylipins, the concentrations increased less rapidly and to a lesser extent compared to the concentrations of their precursor FA. LA-derived oxylipins remained unchanged and arachidonic acid and DHA oxylipin concentrations were not significantly changed. Our results confirm that the intake of ALA is not a sufficient source for the increase of EPA + DHA in subjects on a Western diet. Specifically, a high-ALA diet results in increased EPA and declined DHA concentrations. However, the changes effectively balance each other out so that ΣEPA + DHA in RBCs - which is an established marker for health protective effects of omega-3-PUFA - remains constant. The PUFA levels in RBCs reflect the concentration and its changes in plasma hydroxy- and

EPA Region 1 Environmentally Sensitive Areas

Science.gov (United States)

This coverage represents polygon equivalents of environmentally sensitive areas (ESA) in EPA Region I. ESAs were developed as part of an EPA headquarters initiative based on reviews of various regulatory and guidance documents, as well as phone interviews with federal/state/local government agencies and private organizations. ESAs include, but are not limited to, wetlands, biological resources, habitats, national parks, archaeological/historic sites, natural heritage areas, tribal lands, drinking water intakes, marinas/boat ramps, wildlife areas, etc.

40 CFR 725.370 - EPA review of the TME application.

Science.gov (United States)

2010-07-01

... unreasonable risk of injury to health and the environment as a result of test marketing. ... Marketing § 725.370 EPA review of the TME application. General procedures for review of all submissions... with test marketing activities after receipt of EPA approval. (c) In approving a TME application, EPA...

EPA ENERGY STAR: Tackling Growth in Home Electronics and Small Appliances

Energy Technology Data Exchange (ETDEWEB)

Sanchez, Marla Christine; Brown, Richard; Homan, Gregory

2008-11-17

Over a decade ago, the electricity consumption associated with home electronics and other small appliances emerged onto the global energy policy landscape as one of the fastest growing residential end uses with the opportunity to deliver significant energy savings. As our knowledge of this end use matures, it is essential to step back and evaluate the degree to which energy efficiency programs have successfully realized energy savings and where savings opportunities have been missed.For the past fifteen years, we have quantified energy, utility bill, and carbon savings for US EPA?s ENERGY STAR voluntary product labeling program. In this paper, we present a unique look into the US residential program savings claimed to date for EPA?s ENERGY STAR office equipment, consumer electronics, and other small household appliances as well as EPA?s projected program savings over the next five years. We present a top-level discussion identifying program areas where EPA?s ENERGY STAR efforts have succeeded and program areas where ENERGY STAR efforts did not successfully address underlying market factors, technology issues and/or consumer behavior. We end by presenting the magnitude of ?overlooked? savings.

Privacy Act System of Records: Invention Reports Submitted to the EPA, EPA-38

Science.gov (United States)

Learn about the Invention Reports Submitted to the EPA System, including who is covered in the system, the purpose of data collection, routine uses for the system's records, and other security procedures.

US EPA CARE Grants

Data.gov (United States)

U.S. Environmental Protection Agency — This is a provisional dataset that contains point locations for the subset of Community Action for a Renewed Environment (CARE) grants given out by the US EPA. CARE...

US EPA EJ Grants

Data.gov (United States)

U.S. Environmental Protection Agency — This is a provisional dataset that contains point locations for all Environmental Justice (EJ) grants given out by the US EPA. There are many limitations to the data...

School-based intervention to prevent overweight and disordered eating in secondary school Malaysian adolescents: a study protocol

OpenAIRE

Sharif Ishak, Sharifah Intan Zainun; Chin, Yit Siew; Mohd. Taib, Mohd. Nasir; Mohd. Shariff, Zalilah

2016-01-01

Abstract Background Obesity, eating disorders and unhealthy weight-loss practices have been associated with diminished growth in adolescents worldwide. Interventions that address relevant behavioural dimensions have been lacking in Malaysia. This paper describes the protocol of an integrated health education intervention namely ‘Eat Right, Be Positive About Your Body and Live Actively’ (EPaL), a primary prevention which aimed to promote healthy lifestyle in preventing overweight and disordere...

Proposed modifications of Environmental Protection Agency Method 1601 for detection of coliphages in drinking water, with same-day fluorescence-based detection and evaluation by the performance-based measurement system and alternative test protocol validation approaches.

Science.gov (United States)

Salter, Robert S; Durbin, Gregory W; Conklin, Ernestine; Rosen, Jeff; Clancy, Jennifer

2010-12-01

Coliphages are microbial indicators specified in the Ground Water Rule that can be used to monitor for potential fecal contamination of drinking water. The Total Coliform Rule specifies coliform and Escherichia coli indicators for municipal water quality testing; thus, coliphage indicator use is less common and advances in detection methodology are less frequent. Coliphages are viral structures and, compared to bacterial indicators, are more resistant to disinfection and diffuse further distances from pollution sources. Therefore, coliphage presence may serve as a better predictor of groundwater quality. This study describes Fast Phage, a 16- to 24-h presence/absence modification of U.S. Environmental Protection Agency (EPA) Method 1601 for detection of coliphages in 100 ml water. The objective of the study is to demonstrate that the somatic and male-specific coliphage modifications provide results equivalent to those of Method 1601. Five laboratories compared the modifications, featuring same-day fluorescence-based prediction, to Method 1601 by using the performance-based measurement system (PBMS) criterion. This requires a minimum 50% positive response in 10 replicates of 100-ml water samples at coliphage contamination levels of 1.3 to 1.5 PFU/100 ml. The laboratories showed that Fast Phage meets PBMS criteria with 83.5 to 92.1% correlation of the same-day rapid fluorescence-based prediction with the next-day result. Somatic coliphage PBMS data are compared to manufacturer development data that followed the EPA alternative test protocol (ATP) validation approach. Statistical analysis of the data sets indicates that PBMS utilizes fewer samples than does the ATP approach but with similar conclusions. Results support testing the coliphage modifications by using an EPA-approved national PBMS approach with collaboratively shared samples.

Science Inventory | US EPA

Science.gov (United States)

The Science Inventory is a searchable database of research products primarily from EPA's Office of Research and Development. Science Inventory records provide descriptions of the product, contact information, and links to available printed material or websites.

Centrifugation protocols: tests to determine optimal lithium heparin and citrate plasma sample quality.

Science.gov (United States)

Dimeski, Goce; Solano, Connie; Petroff, Mark K; Hynd, Matthew

2011-05-01

Currently, no clear guidelines exist for the most appropriate tests to determine sample quality from centrifugation protocols for plasma sample types with both lithium heparin in gel barrier tubes for biochemistry testing and citrate tubes for coagulation testing. Blood was collected from 14 participants in four lithium heparin and one serum tube with gel barrier. The plasma tubes were centrifuged at four different centrifuge settings and analysed for potassium (K(+)), lactate dehydrogenase (LD), glucose and phosphorus (Pi) at zero time, poststorage at six hours at 21 °C and six days at 2-8°C. At the same time, three citrate tubes were collected and centrifuged at three different centrifuge settings and analysed immediately for prothrombin time/international normalized ratio, activated partial thromboplastin time, derived fibrinogen and surface-activated clotting time (SACT). The biochemistry analytes indicate plasma is less stable than serum. Plasma sample quality is higher with longer centrifugation time, and much higher g force. Blood cells present in the plasma lyse with time or are damaged when transferred in the reaction vessels, causing an increase in the K(+), LD and Pi above outlined limits. The cells remain active and consume glucose even in cold storage. The SACT is the only coagulation parameter that was affected by platelets >10 × 10(9)/L in the citrate plasma. In addition to the platelet count, a limited but sensitive number of assays (K(+), LD, glucose and Pi for biochemistry, and SACT for coagulation) can be used to determine appropriate centrifuge settings to consistently obtain the highest quality lithium heparin and citrate plasma samples. The findings will aid laboratories to balance the need to provide the most accurate results in the best turnaround time.

Radon in large buildings: The development of a protocol

International Nuclear Information System (INIS)

Wilson, D.L.; Dudney, C.S.; Gammage, R.B.

1993-01-01

Over the past several years, considerable research has been devoted by the US Environmental Protection Agency (USEPA) and others to develop radon sampling protocols for single family residences and schools. However, very little research has been performed on measuring radon in the work place. To evaluate possible sampling protocols, 833 buildings throughout the United States were selected for extensive radon testing. The buildings tested (warehouses, production plants and office buildings) were representative of commercial buildings across the country both in design, size and use. Based on the results, preliminary radon sampling protocols for the work place have been developed

Poster: Report Fraud Waste and Abuse to the EPA OIG Hotline

Science.gov (United States)

The EPA OIG hotline receives complaints of fraud, waste, and abuse in EPA and U.S. Chemical Safety Board programs and operations including mismanagement or violations of law, rules, or regulations by EPA employees or program participants.

Collaborating with EPA through the Federal Technology Transfer Act

Science.gov (United States)

Under the Federal Technology Transfer Act (FTTA), EPA can collaborate with external parties on research projects, and share research materials. Learn more about the types of partnerships the EPA offers.

Active SAmpling Protocol (ASAP) to Optimize Individual Neurocognitive Hypothesis Testing: A BCI-Inspired Dynamic Experimental Design.

Science.gov (United States)

Sanchez, Gaëtan; Lecaignard, Françoise; Otman, Anatole; Maby, Emmanuel; Mattout, Jérémie

2016-01-01

The relatively young field of Brain-Computer Interfaces has promoted the use of electrophysiology and neuroimaging in real-time. In the meantime, cognitive neuroscience studies, which make extensive use of functional exploration techniques, have evolved toward model-based experiments and fine hypothesis testing protocols. Although these two developments are mostly unrelated, we argue that, brought together, they may trigger an important shift in the way experimental paradigms are being designed, which should prove fruitful to both endeavors. This change simply consists in using real-time neuroimaging in order to optimize advanced neurocognitive hypothesis testing. We refer to this new approach as the instantiation of an Active SAmpling Protocol (ASAP). As opposed to classical (static) experimental protocols, ASAP implements online model comparison, enabling the optimization of design parameters (e.g., stimuli) during the course of data acquisition. This follows the well-known principle of sequential hypothesis testing. What is radically new, however, is our ability to perform online processing of the huge amount of complex data that brain imaging techniques provide. This is all the more relevant at a time when physiological and psychological processes are beginning to be approached using more realistic, generative models which may be difficult to tease apart empirically. Based upon Bayesian inference, ASAP proposes a generic and principled way to optimize experimental design adaptively. In this perspective paper, we summarize the main steps in ASAP. Using synthetic data we illustrate its superiority in selecting the right perceptual model compared to a classical design. Finally, we briefly discuss its future potential for basic and clinical neuroscience as well as some remaining challenges.

Feasibility study for the computerized automation of the Laboratory Services Branch of EPA Region IV

International Nuclear Information System (INIS)

Boyle, W.G. Jr.; Barton, G.W. Jr.; Taber, L.

1978-01-01

This report is a study of the feasibility of computerized automation of the Laboratory Services Branch of the Environmental Protection Agency's Region IV. The LSB provides chemical analytical support for a number of EPA divisions; its primary function at present is compliance monitoring, field surveys, and oil identification. Automation of the LSB is not only feasible but also highly desirable. Automation systems are proposed that will make major improvements in analytical capacity, quality control, sample management, and reporting capabilities. Most of these automation systems are similar to those already developed and installed at other EPA laboratories. These systems have options that include limited modifications suggested as a result of the study of the LSB Laboratory, and also include communications hardware and software for a Sample File Control host computer. It is estimated that the initial cost of three of the four options considered would be recouped in approximately three years through increased capacity and efficiency of operation

Hispanos en la EPA: Elias Rodríguez

Science.gov (United States)

La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente.

Hispanos en la EPA: Sally Gutiérrez

Science.gov (United States)

La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente.

EPA's Revised Interim Financial Assistance Conflict of Interest Policy

Science.gov (United States)

EPA has established the following revised interim policy governing disclosure of actual and potential conflicts of interest (COI Policy) by applicants for, and recipients of, federal financial assistance awards from EPA.

IG Statement: Arthur A. Elkins, Jr., on OIG report Early Warning Report: Main EPA Headquarters Warehouse in Landover, Maryland, Requires Immediate EPA Attention

Science.gov (United States)

Statement of Inspector General Arthur A. Elkins, Jr., on the Office of Inspector General (OIG) report Early Warning Report: Main EPA Headquarters Warehouse in Landover, Maryland, Requires Immediate EPA Attention.

Tissue Sampling Guides for Porcine Biomedical Models.

Science.gov (United States)

Albl, Barbara; Haesner, Serena; Braun-Reichhart, Christina; Streckel, Elisabeth; Renner, Simone; Seeliger, Frank; Wolf, Eckhard; Wanke, Rüdiger; Blutke, Andreas

2016-04-01

This article provides guidelines for organ and tissue sampling adapted to porcine animal models in translational medical research. Detailed protocols for the determination of sampling locations and numbers as well as recommendations on the orientation, size, and trimming direction of samples from ∼50 different porcine organs and tissues are provided in the Supplementary Material. The proposed sampling protocols include the generation of samples suitable for subsequent qualitative and quantitative analyses, including cryohistology, paraffin, and plastic histology; immunohistochemistry;in situhybridization; electron microscopy; and quantitative stereology as well as molecular analyses of DNA, RNA, proteins, metabolites, and electrolytes. With regard to the planned extent of sampling efforts, time, and personnel expenses, and dependent upon the scheduled analyses, different protocols are provided. These protocols are adjusted for (I) routine screenings, as used in general toxicity studies or in analyses of gene expression patterns or histopathological organ alterations, (II) advanced analyses of single organs/tissues, and (III) large-scale sampling procedures to be applied in biobank projects. Providing a robust reference for studies of porcine models, the described protocols will ensure the efficiency of sampling, the systematic recovery of high-quality samples representing the entire organ or tissue as well as the intra-/interstudy comparability and reproducibility of results. © The Author(s) 2016.

EPA Region 1 Coast Guard Jurisdictional Boundary - Polygons

Science.gov (United States)

Jurisdictional boundary between EPA and Coast Guard for EPA Region I. Created from 1:100000 USGS DLGs with greater detail drawn from 1:24000 commercial street data for Region I.This layer is used to determine which agency will be reponsible in the event of an oil spill.

EPA Region 1 Coast Guard Jurisdictional Boundary - Arcs

Science.gov (United States)

Jurisdictional boundary between EPA and Coast Guard for EPA Region I. Created from 1:100000 USGS DLGs with greater detail drawn from 1:24000 commercial street data for Region I.This layer is used to determine which agency will be reponsible in the event of an oil spill.

Notification: EPA Investments in Information Technology Products and Services

Science.gov (United States)

Project #OA-FY14-0307, June 10, 2014. The U.S. Environmental Protection Agency (EPA) Office oflnspector General (OIG) plans to begin preliminary research on the EPA's management of information technology (IT) investments.

US EPA's Ecological Risk Assessment Support Center ...

Science.gov (United States)

BackgroundThe ERASC provides technical information and addresses scientific questions of concern or interest on topics relevant to ecological risk assessment at hazardous waste sites for EPA's Office of Solid Waste and Emergency Response (OSWER) personnel and the Office of Resource Conservation and Recovery (ORCR) staff. Requests are channeled to ERASC through the Ecological Risk Assessment Forum (ERAF). To assess emerging and complex scientific issues that require expert judgment, the ERASC relies on the expertise of scientists and engineers located throughout EPA's Office of Research and Development (ORD) labs and centers.ResponseERASC develops responses that reflect the state of the science for ecological risk assessment and also provides a communication point for the distribution of the responses to other interested parties. For further information, contact Ecology_ERASC@epa.gov or call 513-569-7940.

EPA Web Training Classes

Science.gov (United States)

Scheduled webinars can help you better manage EPA web content. Class topics include Drupal basics, creating different types of pages in the WebCMS such as document pages and forms, using Google Analytics, and best practices for metadata and accessibility.

UMTRA project water sampling and analysis plan, Tuba City, Arizona

International Nuclear Information System (INIS)

1996-02-01

Planned, routine ground water sampling activities at the U.S. Department of Energy (DOE) Uranium Mill Tailings Remedial Action (UMTRA) Project site in Tuba City, Arizona, are described in the following sections of this water sampling and analysis plan (WSAP). This plan identifies and justifies the sampling locations, analytical parameters, detection limits, and sampling frequency for the stations routinely monitored at the site. The ground water data are used for site characterization and risk assessment. The regulatory basis for routine ground water monitoring at UMTRA Project sites is derived from the U.S. Environmental Protection Agency (EPA) regulations in 40 CFR Part 192 (1994) and the final EPA standards of 1995 (60 FR 2854). Sampling procedures are guided by the UMTRA Project standard operating procedures (SOP) (JEG, n.d.), and the most effective technical approach for the site

Omega-3 polyunsaturated fatty acid blood biomarkers increase linearly in men and women after tightly controlled intakes of 0.25, 0.5, and 1 g/d of EPA + DHA.

Science.gov (United States)

Patterson, Ashley C; Chalil, Alan; Aristizabal Henao, Juan J; Streit, Isaac T; Stark, Ken D

2015-12-01

Blood levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been related to coronary heart disease risk. Understanding the response of EPA + DHA in blood to dietary intake of EPA + DHA would facilitate the use of blood measures as markers of adherence and enable the development of dietary recommendations. The objective of this study is examine the blood response to intakes of EPA + DHA ≤1 g/d with an intervention designed for dietary adherence. It was hypothesized this relationship would be linear and that intakes of EPA + DHA DHA intake of men and women (n = 20) was determined by food frequency questionnaire and adherence was monitored by weekly fingertip blood sampling for fatty acid determinations. Participants consumed nutraceuticals to achieve intakes of 0.25 g/d and 0.5 g/d EPA + DHA for successive four-week periods. A subgroup (n = 5) had intakes of 1.0 g/d EPA + DHA for an additional 4 weeks. Fatty acid composition of whole blood, erythrocytes, and plasma phospholipids were determined at each time point. Blood levels of EPA and DHA increased linearly in these pools. A comprehensive review of the literature was used to verify the blood-intake relationship. Blood levels of long chain omega-3 polyunsaturated fatty acids reached blood levels associated with the highest levels of primary cardiac arrest reduction and sudden cardiac death risk only with intakes of 1.0 g/d of EPA + DHA. The blood biomarker response to intakes of EPA + DHA ≤1 g/d is linear in a small but highly adherent study sample and this information can assist in determining adherence in clinical studies and help identify dietary intake targets from associations between blood and disease. Copyright © 2015 Elsevier Inc. All rights reserved.

From Marshes to the Continental Shelf: Results of the Western Component of the US EPA National Coastal Assessment

Science.gov (United States)

W. G. Nelson; H. II Lee; J. O. Lamberson

2006-01-01

The National Coastal Assessment of the US EPA began field work in the Western US in 1999-2000. Probabilistic sampling for biotic and abiotic condition indicators was conducted at 381 stations within estuaries and coastal embayments of Washington, Oregon and California. In 2002, intertidal and low salt marsh habitats were sampled at an additional 190 stations. As part...

Notification: Audit of Certain EPA Electronic Records Management Practices

Science.gov (United States)

Project #OA-FY13-0113, December 13, 2012. This memorandum is to notify you that the U.S. Environmental Protection Agency (EPA), Office of Inspector General, plans to begin an audit of certain EPA electronic records management practices.

40 CFR 600.109-78 - EPA driving cycles.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 29 2010-07-01 2010-07-01 false EPA driving cycles. 600.109-78 Section... Model Year Automobiles-Test Procedures § 600.109-78 EPA driving cycles. (a) The driving cycle to be... driving cycle to be utilized for generation of the highway fuel economy data is specified in this...

EPA Collaboration with South Korea

Science.gov (United States)

EPA, the Ministry of Environment of Korea, and partner agencies in both countries cooperate to strengthen environmental governance, improve air and water quality, and reduce exposure to toxic chemicals.

40 CFR 2.205 - Final confidentiality determination by EPA legal office.

Science.gov (United States)

2010-07-01

..., after consideration of any timely comments submitted by the business, the EPA legal office makes a... is made by the business and approved by the EPA legal office. Except in extraordinary circumstances... determinations; matters to be considered. (1) If the EPA legal office finds that a business has failed to furnish...

Work-In-Progress Peer Consult on EPA's Multimedia ...

Science.gov (United States)

This document is a compilation of responses from four external peer reviewers on EPA's "Multimedia Exposure Analysis to Inform a Public Health-Based Value for Lead in Drinking Water." It was delivered by Versar, Inc. under contract number EP-C-12-045 Task Order 91. Peer review report compiled, written and delivered by Versar, Inc to EPA.

40 CFR 600.109-08 - EPA driving cycles.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 29 2010-07-01 2010-07-01 false EPA driving cycles. 600.109-08 Section... Model Year Automobiles-Test Procedures § 600.109-08 EPA driving cycles. (a) The FTP driving cycle is prescribed in § 86.115 of this chapter. (b) The highway fuel economy driving cycle is specified in this...

National Air Toxics Assessment - 2005, EPA Region 2 (EPA.AIR.NATA99_R2)

Data.gov (United States)

U.S. Environmental Protection Agency — This data layer is based on the model results of the 1999 National-Scale Assessment (N-SA), a part of the National Air Toxics Assessment (NATA), conducted by EPA's...

National Air Toxics Assessment - 2002, EPA Region 2 (EPA.AIR.NATA99_R2)

Data.gov (United States)

U.S. Environmental Protection Agency — This data layer is based on the model results of the 1999 National-Scale Assessment (N-SA), a part of the National Air Toxics Assessment (NATA), conducted by EPA's...

National Air Toxics Assessment - 1999, EPA Region 2 (EPA.AIR.NATA99_R2)

Data.gov (United States)

U.S. Environmental Protection Agency — This data layer is based on the model results of the 1999 National-Scale Assessment (N-SA), a part of the National Air Toxics Assessment (NATA), conducted by EPA's...

Surface and subsurface cleanup protocol for radionuclides, Gunnison, Colorado, UMTRA project processing site

International Nuclear Information System (INIS)

Gonzales, D.

1993-12-01

Surface and subsurface soil cleanup protocols for the Gunnison, Colorado, processing site are summarized as follows: In accordance with EPA-promulgated land cleanup standards, in situ Ra-226 is to be cleaned up based on bulk concentrations not exceeding 5 and 15 pCi/g in 15-cm surface and subsurface depth increments, averaged over 100m 2 grid blocks, where the parent Ra-226 concentrations are greater than, or in secular equilibrium with, the Th-230 parent. In locations where Th-230 has differentially migrated in subsoil relative to Ra-226, a Th-230 clean up protocol has been developed. The cleanup of other radionuclides or nonradiological hazards that pose a significant threat to the public and the environment will be determined and implemented in accordance with pathway analysis to assess impacts and the implications of ALARA specified in 40 CFR Part 192 relative to supplemental standards

The relation between the omega-3 index and arachidonic acid is bell shaped : Synergistic at low EPA plus DHA status and antagonistic at high EPA plus DHA status

NARCIS (Netherlands)

Luxwolda, Martine F.; Kuipers, Remko S.; Smit, Ella N.; Velzing-Aarts, Francien V.; Dijck-Brouwer, D. A. Janneke; Muskiet, Frits A. J.

2011-01-01

Introduction: The relation between docosahexaenoic (DHA) and eicosapentaenoic (EPA) vs. arachidonic acid (AA) seems characterized by both synergism and antagonism. Materials and methods: Investigate the relation between EPA + DHA and AA in populations with a wide range of EPA + DHA status and across

EPA RE-Powering Screening Shapefile

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Center for Program Analysis (CPA) initiated the RE-Powering America’s...

Modifying EPA radiation risk models based on BEIR VII

International Nuclear Information System (INIS)

Pawel, D.; Puskin, J.

2007-01-01

This paper summarizes a 'draft White Paper' that provides details on proposed changes in EPA's methodology for estimating radiogenic cancer risks. Many of the changes are based on the contents of a recent National Academy of Sciences (NAS) report (BEIR VII), that addresses cancer and genetic risks from low doses of low-LET radiation. The draft White Paper was prepared for a meeting with the EPA's Science Advisory Board's Radiation Advisory Committee (RAC) in September for seeking advice on the application of BEIR VII and on issues relating to these modifications and expansions. After receiving the Advisory review, we plan to implement the changes by publishing the new methodology in an EPA report, which we expect to submit to the RAC for final review. The revised methodology could then be applied to update the cancer risk coefficients for over 800 radionuclides that are published in EPA's Federal Guidance Report 13. (author)

EPA's Green Roof Research

Science.gov (United States)

This is a presentation on the basics of green roof technology. The presentation highlights some of the recent ORD research projects on green roofs and provices insight for the end user as to the benefits for green roof technology. It provides links to currently available EPA re...

Green Roof Research through EPA's Regional Applied Research Effort

Science.gov (United States)

ABSTRACT The U.S. Environmental Protection Agency’s (EPA) Regional Applied Research Effort (RARE) allows the Regions of the EPA to choose research projects to be performed in partnership with EPA’s Office of Research and Development (ORD). Over the last decade, several green roo...

Strategies for achieving high sequencing accuracy for low diversity samples and avoiding sample bleeding using illumina platform.

Science.gov (United States)

Mitra, Abhishek; Skrzypczak, Magdalena; Ginalski, Krzysztof; Rowicka, Maga

2015-01-01

Sequencing microRNA, reduced representation sequencing, Hi-C technology and any method requiring the use of in-house barcodes result in sequencing libraries with low initial sequence diversity. Sequencing such data on the Illumina platform typically produces low quality data due to the limitations of the Illumina cluster calling algorithm. Moreover, even in the case of diverse samples, these limitations are causing substantial inaccuracies in multiplexed sample assignment (sample bleeding). Such inaccuracies are unacceptable in clinical applications, and in some other fields (e.g. detection of rare variants). Here, we discuss how both problems with quality of low-diversity samples and sample bleeding are caused by incorrect detection of clusters on the flowcell during initial sequencing cycles. We propose simple software modifications (Long Template Protocol) that overcome this problem. We present experimental results showing that our Long Template Protocol remarkably increases data quality for low diversity samples, as compared with the standard analysis protocol; it also substantially reduces sample bleeding for all samples. For comprehensiveness, we also discuss and compare experimental results from alternative approaches to sequencing low diversity samples. First, we discuss how the low diversity problem, if caused by barcodes, can be avoided altogether at the barcode design stage. Second and third, we present modified guidelines, which are more stringent than the manufacturer's, for mixing low diversity samples with diverse samples and lowering cluster density, which in our experience consistently produces high quality data from low diversity samples. Fourth and fifth, we present rescue strategies that can be applied when sequencing results in low quality data and when there is no more biological material available. In such cases, we propose that the flowcell be re-hybridized and sequenced again using our Long Template Protocol. Alternatively, we discuss how

Strategies for achieving high sequencing accuracy for low diversity samples and avoiding sample bleeding using illumina platform.

Directory of Open Access Journals (Sweden)

Abhishek Mitra

Full Text Available Sequencing microRNA, reduced representation sequencing, Hi-C technology and any method requiring the use of in-house barcodes result in sequencing libraries with low initial sequence diversity. Sequencing such data on the Illumina platform typically produces low quality data due to the limitations of the Illumina cluster calling algorithm. Moreover, even in the case of diverse samples, these limitations are causing substantial inaccuracies in multiplexed sample assignment (sample bleeding. Such inaccuracies are unacceptable in clinical applications, and in some other fields (e.g. detection of rare variants. Here, we discuss how both problems with quality of low-diversity samples and sample bleeding are caused by incorrect detection of clusters on the flowcell during initial sequencing cycles. We propose simple software modifications (Long Template Protocol that overcome this problem. We present experimental results showing that our Long Template Protocol remarkably increases data quality for low diversity samples, as compared with the standard analysis protocol; it also substantially reduces sample bleeding for all samples. For comprehensiveness, we also discuss and compare experimental results from alternative approaches to sequencing low diversity samples. First, we discuss how the low diversity problem, if caused by barcodes, can be avoided altogether at the barcode design stage. Second and third, we present modified guidelines, which are more stringent than the manufacturer's, for mixing low diversity samples with diverse samples and lowering cluster density, which in our experience consistently produces high quality data from low diversity samples. Fourth and fifth, we present rescue strategies that can be applied when sequencing results in low quality data and when there is no more biological material available. In such cases, we propose that the flowcell be re-hybridized and sequenced again using our Long Template Protocol. Alternatively

Effect of variable rates of daily sampling of fly larvae on decomposition and carrion insect community assembly: implications for forensic entomology field study protocols.

Science.gov (United States)

Michaud, Jean-Philippe; Moreau, Gaétan

2013-07-01

Experimental protocols in forensic entomology successional field studies generally involve daily sampling of insects to document temporal changes in species composition on animal carcasses. One challenge with that method has been to adjust the sampling intensity to obtain the best representation of the community present without affecting the said community. To this date, little is known about how such investigator perturbations affect decomposition-related processes. Here, we investigated how different levels of daily sampling of fly eggs and fly larvae affected, over time, carcass decomposition rate and the carrion insect community. Results indicated that a daily sampling of forensic entomology successional field studies.

Enforcement Alert: Hazardous Waste Management Practices at Mineral Processing Facilities Under Scrutiny by U.S. EPA; EPA Clarifies 'Bevill Exclusion' Wastes and Establishes Disposal Standards

Science.gov (United States)

This is the enforcement alert for Hazardous Waste Management Practices at Mineral Processing Facilities Under Scrutiny by U.S. EPA; EPA Clarifies 'Bevill Exclusion' Wastes and Establishes Disposal Standards

Stream sediment sampling and analysis. Final report

International Nuclear Information System (INIS)

Means, J.L.; Voris, P.V.; Headington, G.L.

1986-04-01

The objectives were to sample and analyze sediments from upstream and downstream locations (relative to the Goodyear Atomic plant site) of three streams for selected pollutants. The three streams sampled were the Scioto River, Big Beaver Creek, and Big Run Creek. Sediment samples were analyzed for EPA's 129 priority pollutants (Clean Water Act) as well as isotopic uranium ( 234 U, 235 U, and 238 U) and technetium-99

UMTRA Project water sampling and analysis plan, Grand Junction, Colorado. Revision 1, Version 6

International Nuclear Information System (INIS)

1995-09-01

This water sampling and analysis plan describes the planned, routine ground water sampling activities at the Grand Junction US DOE Uranium Mill Tailings Remedial Action (UMTRA) Project site (GRJ-01) in Grand Junction, Colorado, and at the Cheney Disposal Site (GRJ-03) near Grand Junction. The plan identifies and justifies the sampling locations, analytical parameters, detection limits, and sampling frequencies for the routine monitoring stations at the sites. Regulatory basis is in the US EPA regulations in 40 CFR Part 192 (1994) and EPA ground water quality standards of 1995 (60 FR 2854). This plan summarizes results of past water sampling activities, details water sampling activities planned for the next 2 years, and projects sampling activities for the next 5 years

Hispanos en la EPA: Rafael DeLeón

Science.gov (United States)

La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente.

US EPA's SPECIATE 4.4 Database: Development and Uses

Science.gov (United States)

SPECIATE is the U.S. Environmental Protection Agency’s (EPA) repository of volatile organic gas and particulate matter (PM) speciation profiles of air pollution sources. EPA released SPECIATE 4.4 in early 2014 and, in total, the SPECIATE 4.4 database includes 5,728 PM, volatile o...

40 CFR 74.10 - Roles-EPA and permitting authority.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 16 2010-07-01 2010-07-01 false Roles-EPA and permitting authority. 74... (CONTINUED) SULFUR DIOXIDE OPT-INS Permitting Procedures § 74.10 Roles—EPA and permitting authority. (a... end-of-year compliance, determining reduced utilization, approving thermal energy transfer and...

EPA Nonregulatory Nonroad Duty Cycles

Science.gov (United States)

EPA nonregulatory, nonroad duty cycles for equipment such as agricultural tractors, backhoe loaders,crawlers tractors, excavators, arc welding skid steer loaders, and wheel loaders. Also,test procedures, laboratory methods, and emissions for this equipmen

EPA (Environmental Protection Agency) Method Study 12, cyanide in water. Final report

Energy Technology Data Exchange (ETDEWEB)

Winter, J.; Britton, P.; Kroner, R.

1984-05-01

EPA Method Study 12, Cyanide in Water reports the results of a study by EMSL-Cincinnati for the parameters, Total Cyanide and Cyanides Amendable to Chlorination, present in water at microgram per liter levels. Four methods: pyridine-pyrazolone, pyridine-barbituric acid, electrode and Roberts-Jackson were used by 112 laboratories in Federal and State agencies, municipalities, universities, and the private/industrial sector. Sample concentrates were prepared in pairs with similar concentrations at each of three levels. Analysts diluted samples to volume with distilled and natural waters and analyzed them. Precision, accuracy, bias and the natural water interference were evaluated for each analytical method and comparisons were made between the four methods.

Interim Basis for PCB Sampling and Analyses

International Nuclear Information System (INIS)

BANNING, D.L.

2001-01-01

This document was developed as an interim basis for sampling and analysis of polychlorinated biphenyls (PCBs) and will be used until a formal data quality objective (DQO) document is prepared and approved. On August 31, 2000, the Framework Agreement for Management of Polychlorinated Biphenyls (PCBs) in Hanford Tank Waste was signed by the US. Department of Energy (DOE), the Environmental Protection Agency (EPA), and the Washington State Department of Ecology (Ecology) (Ecology et al. 2000). This agreement outlines the management of double shell tank (DST) waste as Toxic Substance Control Act (TSCA) PCB remediation waste based on a risk-based disposal approval option per Title 40 of the Code of Federal Regulations 761.61 (c). The agreement calls for ''Quantification of PCBs in DSTs, single shell tanks (SSTs), and incoming waste to ensure that the vitrification plant and other ancillary facilities PCB waste acceptance limits and the requirements of the anticipated risk-based disposal approval are met.'' Waste samples will be analyzed for PCBs to satisfy this requirement. This document describes the DQO process undertaken to assure appropriate data will be collected to support management of PCBs and is presented in a DQO format. The DQO process was implemented in accordance with the U.S. Environmental Protection Agency EPA QAlG4, Guidance for the Data Quality Objectives Process (EPA 1994) and the Data Quality Objectives for Sampling and Analyses, HNF-IP-0842/Rev.1 A, Vol. IV, Section 4.16 (Banning 1999)

Dysphonia risk screening protocol

Science.gov (United States)

Nemr, Katia; Simões-Zenari, Marcia; da Trindade Duarte, João Marcos; Lobrigate, Karen Elena; Bagatini, Flavia Alves

2016-01-01

OBJECTIVE: To propose and test the applicability of a dysphonia risk screening protocol with score calculation in individuals with and without dysphonia. METHOD: This descriptive cross-sectional study included 365 individuals (41 children, 142 adult women, 91 adult men and 91 seniors) divided into a dysphonic group and a non-dysphonic group. The protocol consisted of 18 questions and a score was calculated using a 10-cm visual analog scale. The measured value on the visual analog scale was added to the overall score, along with other partial scores. Speech samples allowed for analysis/assessment of the overall degree of vocal deviation and initial definition of the respective groups and after six months, the separation of the groups was confirmed using an acoustic analysis. RESULTS: The mean total scores were different between the groups in all samples. Values ranged between 37.0 and 57.85 in the dysphonic group and between 12.95 and 19.28 in the non-dysphonic group, with overall means of 46.09 and 15.55, respectively. High sensitivity and specificity were demonstrated when discriminating between the groups with the following cut-off points: 22.50 (children), 29.25 (adult women), 22.75 (adult men), and 27.10 (seniors). CONCLUSION: The protocol demonstrated high sensitivity and specificity in differentiating groups of individuals with and without dysphonia in different sample groups and is thus an effective instrument for use in voice clinics. PMID:27074171

Dysphonia risk screening protocol

Directory of Open Access Journals (Sweden)

Katia Nemr

2016-03-01

Full Text Available OBJECTIVE: To propose and test the applicability of a dysphonia risk screening protocol with score calculation in individuals with and without dysphonia. METHOD: This descriptive cross-sectional study included 365 individuals (41 children, 142 adult women, 91 adult men and 91 seniors divided into a dysphonic group and a non-dysphonic group. The protocol consisted of 18 questions and a score was calculated using a 10-cm visual analog scale. The measured value on the visual analog scale was added to the overall score, along with other partial scores. Speech samples allowed for analysis/assessment of the overall degree of vocal deviation and initial definition of the respective groups and after six months, the separation of the groups was confirmed using an acoustic analysis. RESULTS: The mean total scores were different between the groups in all samples. Values ranged between 37.0 and 57.85 in the dysphonic group and between 12.95 and 19.28 in the non-dysphonic group, with overall means of 46.09 and 15.55, respectively. High sensitivity and specificity were demonstrated when discriminating between the groups with the following cut-off points: 22.50 (children, 29.25 (adult women, 22.75 (adult men, and 27.10 (seniors. CONCLUSION: The protocol demonstrated high sensitivity and specificity in differentiating groups of individuals with and without dysphonia in different sample groups and is thus an effective instrument for use in voice clinics.

EPA`s program for risk assessment guidelines: Quantification issues

Energy Technology Data Exchange (ETDEWEB)

Dourson, M.L. [Environmental Protection Agency, Washington, DC (United States)

1990-12-31

The quantitative procedures associated with noncancer risk assessment include reference dose (RfD), benchmark dose, and severity modeling. The RfD, which is part of the EPA risk assessment guidelines, is an estimation of a level that is likely to be without any health risk to sensitive individuals. The RfD requires two major judgments: the first is choice of a critical effect(s) and its No Observed Adverse Effect Level (NOAEL); the second judgment is choice of an uncertainty factor. This paper discusses major assumptions and limitations of the RfD model.

Headache symptoms and indoor environmental parameters: Results from the EPA BASE study

Directory of Open Access Journals (Sweden)

Gretchen E Tietjen

2012-01-01

Full Text Available Objective: The objective of this investigation was to determine the prevalence of migraine and headache symptoms in a national sample of US office employees. Also, we explored the association of headache symptoms with indoor environmental parameters of the work place. Background: Sick building syndrome (SBS, which includes headache, is a common global phenomenon, but the underlying environmental cause is uncertain. Materials and Methods: We used data from the 1994-1998 US Environmental Protection Agency′s (EPA Building Assessment and Survey Evaluation, a cross-sectional study of workers employed in 100 public and private office buildings across 25 states. The study used a self-administered questionnaire to assess headache frequency and prevalence of self-reported physician-diagnosed (SRPD migraine. Indoor environmental parameters (IEP were collected per EPA protocol from each building over a 1-week period and included carbon dioxide, carbon monoxide, temperature, relative humidity, particulate matter, volatile organic compound, illuminance, and sound level. The standards of American Society of Heating, Refrigerating and Air Conditioning Engineers were used to categorize IEP as either within- or out-of-comfort range for human dwelling. These limits delineate whether a parameter value is safe for human dwelling. Out-of-comfort range IEPs are associated with SBS and other human diseases. SRPD migraine and headache frequency were the primary outcome measures of the study. Multivariate logistic regression analyses were employed for the purpose of assessing the association between the outcome variable and IEPs. Results: Of the 4326 participants, 66% were females and 60% were between 30 and 49 years. Headache frequency during the last 4 weeks was as follows: None in 31%, 1-3 days in 38%, 1-3 days per week in 18%, and every or almost every workday in 8%. Females had higher SRPD migraine prevalence compared to males (27% vs. 11%, P<0.001 and were more

21 CFR 660.6 - Samples; protocols; official release.

Science.gov (United States)

2010-04-01

... product iodinated with 125I means a sample from each lot of diagnostic test kits in a finished package... manufacturer has satisfactorily completed all tests on the samples: (i) One sample until written notification... of this section, a sample of product not iodinated with 125I means a sample from each filling of each...

Innovative methods for inorganic sample preparation

Energy Technology Data Exchange (ETDEWEB)

Essling, A.M.; Huff, E.A.; Graczyk, D.G.

1992-04-01

Procedures and guidelines are given for the dissolution of a variety of selected materials using fusion, microwave, and Parr bomb techniques. These materials include germanium glass, corium-concrete mixtures, and zeolites. Emphasis is placed on sample-preparation approaches that produce a single master solution suitable for complete multielement characterization of the sample. In addition, data are presented on the soil microwave digestion method approved by the Environmental Protection Agency (EPA). Advantages and disadvantages of each sample-preparation technique are summarized.

Innovative methods for inorganic sample preparation

International Nuclear Information System (INIS)

Essling, A.M.; Huff, E.A.; Graczyk, D.G.

1992-04-01

Procedures and guidelines are given for the dissolution of a variety of selected materials using fusion, microwave, and Parr bomb techniques. These materials include germanium glass, corium-concrete mixtures, and zeolites. Emphasis is placed on sample-preparation approaches that produce a single master solution suitable for complete multielement characterization of the sample. In addition, data are presented on the soil microwave digestion method approved by the Environmental Protection Agency (EPA). Advantages and disadvantages of each sample-preparation technique are summarized

Memorandum of Understanding Between U.S. EPA Superfund and U.S. NRC

International Nuclear Information System (INIS)

Walker, Stuart

2008-01-01

The Environmental Protection Agency (EPA) Office of Superfund Remediation and Technology Innovation (OSRTI) and the Nuclear Regulatory Commission (NRC) are responsible for implementing the 'Memorandum of Understanding Between the Environmental Protection Agency and the Nuclear Regulatory Commission: Consultation and Finality on Decommissioning and Decontamination of Contaminated Sites'. This paper provides a brief overview of the origin of the Memorandum of Understanding (MOU), the major features of the MOU, and how the MOU has been implemented site specifically. EPA and NRC developed the MOU in response to direction from the House Committee on Appropriations to EPA and NRC to work together to address the potential for dual regulation. The MOU was signed by EPA on September 30, 2002 and NRC on October 9, 2002. The two agencies had worked on the MOU since March 2000. While both EPA and NRC have statutory authority to clean up these sites, the MOU provides consultation procedures between EPA and NRC to eliminate dual regulation. Under the MOU, EPA and NRC identified the interactions of the two agencies for the decommissioning and decontamination of NRC-licensed sites and the ways in which those responsibilities will be exercised. Except for Section VI, which addresses corrective action under the Resource Conservation and Recovery Act (RCRA), this MOU is limited to the coordination between EPA, when acting under its CERCLA authority, and NRC, when a facility licensed by the NRC is undergoing decommissioning, or when a facility has completed decommissioning, and the NRC has terminated its license. EPA believes that implementation of the MOU between the two agencies will ensure that future confusion about dual regulation does not occur regarding the cleanup and reuse of NRC-licensed sites. NRC and EPA have so far exchanged MOU consultation letters on eight NRC-licensed sites. EPA has responded to each consultation request with a letter expressing its views on actions

Most Recent Sampling Results for Annex III Building

Science.gov (United States)

Contains email from Scott Miller, US EPA to Scott Kramer. Subject: Most Recent Sampling Results for Annex III Building. (2:52 PM) and Gore(TM) Surveys Analytical Results U.S. Geological Survey, Montgomery, AL.

Multiple surveys employing a new sample-processing protocol reveal the genetic diversity of placozoans in Japan.

Science.gov (United States)

Miyazawa, Hideyuki; Nakano, Hiroaki

2018-03-01

Placozoans, flat free-living marine invertebrates, possess an extremely simple bauplan lacking neurons and muscle cells and represent one of the earliest-branching metazoan phyla. They are widely distributed from temperate to tropical oceans. Based on mitochondrial 16S rRNA sequences, 19 haplotypes forming seven distinct clades have been reported in placozoans to date. In Japan, placozoans have been found at nine locations, but 16S genotyping has been performed at only two of these locations. Here, we propose a new processing protocol, "ethanol-treated substrate sampling," for collecting placozoans from natural environments. We also report the collection of placozoans from three new locations, the islands of Shikine-jima, Chichi-jima, and Haha-jima, and we present the distribution of the 16S haplotypes of placozoans in Japan. Multiple surveys conducted at multiple locations yielded five haplotypes that were not reported previously, revealing high genetic diversity in Japan, especially at Shimoda and Shikine-jima Island. The observed geographic distribution patterns were different among haplotypes; some were widely distributed, while others were sampled only from a single location. However, samplings conducted on different dates at the same sites yielded different haplotypes, suggesting that placozoans of a given haplotype do not inhabit the same site constantly throughout the year. Continued sampling efforts conducted during all seasons at multiple locations worldwide and the development of molecular markers within the haplotypes are needed to reveal the geographic distribution pattern and dispersal history of placozoans in greater detail.

Bayesian adaptive survey protocols for resource management

Science.gov (United States)

Halstead, Brian J.; Wylie, Glenn D.; Coates, Peter S.; Casazza, Michael L.

2011-01-01

Transparency in resource management decisions requires a proper accounting of uncertainty at multiple stages of the decision-making process. As information becomes available, periodic review and updating of resource management protocols reduces uncertainty and improves management decisions. One of the most basic steps to mitigating anthropogenic effects on populations is determining if a population of a species occurs in an area that will be affected by human activity. Species are rarely detected with certainty, however, and falsely declaring a species absent can cause improper conservation decisions or even extirpation of populations. We propose a method to design survey protocols for imperfectly detected species that accounts for multiple sources of uncertainty in the detection process, is capable of quantitatively incorporating expert opinion into the decision-making process, allows periodic updates to the protocol, and permits resource managers to weigh the severity of consequences if the species is falsely declared absent. We developed our method using the giant gartersnake (Thamnophis gigas), a threatened species precinctive to the Central Valley of California, as a case study. Survey date was negatively related to the probability of detecting the giant gartersnake, and water temperature was positively related to the probability of detecting the giant gartersnake at a sampled location. Reporting sampling effort, timing and duration of surveys, and water temperatures would allow resource managers to evaluate the probability that the giant gartersnake occurs at sampled sites where it is not detected. This information would also allow periodic updates and quantitative evaluation of changes to the giant gartersnake survey protocol. Because it naturally allows multiple sources of information and is predicated upon the idea of updating information, Bayesian analysis is well-suited to solving the problem of developing efficient sampling protocols for species of

40 CFR 147.1703 - EPA-administered program-Indian lands.

Science.gov (United States)

2010-07-01

... Carolina § 147.1703 EPA-administered program—Indian lands. (a) Contents. The UIC program for all classes of wells on Indian lands in the State of North Carolina is administered by EPA. This program consists of... these requirements. (b) Effective date. The effective date of the UIC program for Indian lands in North...

UMTRA Project water sampling and analysis plan, Durango, Colorado. Revision 1

International Nuclear Information System (INIS)

1995-09-01

Planned, routine ground water sampling activities at the US Department of Energy (DOE) Uranium Mill Tailings Remedial Action (UMTRA) Project site in Durango, Colorado, are described in this water sampling and analysis plan. The plan identifies and justifies the sampling locations, analytical parameters, detection limits, and sampling frequency for the routine monitoring stations at the site. The ground water data are used to characterize the site ground water compliance strategies and to monitor contaminants of potential concern identified in the baseline risk assessment (DOE, 1995a). Regulatory basis for routine ground water monitoring at UMTRA Project sites is derived from the US EPA regulations in 40 CFR Part 192 (1994) and EPA standards of 1995 (60 FR 2854). Sampling procedures are guided by the UMTRA Project standard operating procedures (SOP) (JEG, n.d.), the Technical Approach Document (TAD) (DOE, 1989), and the most effective technical approach for the site

EPA Region 1 - Map Layers for Valley ID Tool (Hosted Feature Service)

Science.gov (United States)

The Valley Service Feature Layer hosts spatial data for EPA Region 1's Valley Identification Tool. These layers contain attribute information added by EPA R1 GIS Center to help identify populated valleys:- Fac_2011NEI: Pollution sources selected from the National Emissions Inventory (EPA, 2011).- NE_Towns_PopValleys: New England Town polygons (courtesy USGS), with Population in Valleys and Population Density in Valleys calculated by EPA R1 GIS, from 2010 US Census blocks. - VT_E911: Vermont residences (courtesy VT Center for Geographic Information E-911).

Experimental Protocol to Determine the Chloride Threshold Value for Corrosion in Samples Taken from Reinforced Concrete Structures.

Science.gov (United States)

Angst, Ueli M; Boschmann, Carolina; Wagner, Matthias; Elsener, Bernhard

2017-08-31

The aging of reinforced concrete infrastructure in developed countries imposes an urgent need for methods to reliably assess the condition of these structures. Corrosion of the embedded reinforcing steel is the most frequent cause for degradation. While it is well known that the ability of a structure to withstand corrosion depends strongly on factors such as the materials used or the age, it is common practice to rely on threshold values stipulated in standards or textbooks. These threshold values for corrosion initiation (Ccrit) are independent of the actual properties of a certain structure, which clearly limits the accuracy of condition assessments and service life predictions. The practice of using tabulated values can be traced to the lack of reliable methods to determine Ccrit on-site and in the laboratory. Here, an experimental protocol to determine Ccrit for individual engineering structures or structural members is presented. A number of reinforced concrete samples are taken from structures and laboratory corrosion testing is performed. The main advantage of this method is that it ensures real conditions concerning parameters that are well known to greatly influence Ccrit, such as the steel-concrete interface, which cannot be representatively mimicked in laboratory-produced samples. At the same time, the accelerated corrosion test in the laboratory permits the reliable determination of Ccrit prior to corrosion initiation on the tested structure; this is a major advantage over all common condition assessment methods that only permit estimating the conditions for corrosion after initiation, i.e., when the structure is already damaged. The protocol yields the statistical distribution of Ccrit for the tested structure. This serves as a basis for probabilistic prediction models for the remaining time to corrosion, which is needed for maintenance planning. This method can potentially be used in material testing of civil infrastructures, similar to established

40 CFR 13.22 - Salary offset when EPA is the creditor agency.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Salary offset when EPA is the creditor... COLLECTION STANDARDS Administrative Offset § 13.22 Salary offset when EPA is the creditor agency. (a... through salary offset, EPA will first provide the employee with the opportunity to pay in full the amount...

NaKnowBaseTM: The EPA Nanomaterials Research ...

Science.gov (United States)

The ability to predict the environmental and health implications of engineered nanomaterials is an important research priority due to the exponential rate at which nanotechnology is being incorporated into consumer, industrial and biomedical applications. To address this need and develop predictive capability, we have created the NaKnowbaseTM, which provides a platform for the curation and dissemination of EPA nanomaterials data to support functional assay development, hazard risk models and informatic analyses. To date, we have combined relevant physicochemical parameters from other organizations (e.g., OECD, NIST), with those requested for nanomaterial data submitted to EPA under the Toxic Substances Control Act (TSCA). Physiochemical characterization data were collated from >400 unique nanomaterials including metals, metal oxides, carbon-based and hybrid materials evaluated or synthesized by EPA researchers. We constructed parameter requirements and table structures for encoding research metadata, including experimental factors and measured response variables. As a proof of concept, we illustrate how SQL-based queries facilitate a range of interrogations including, for example, relationships between nanoparticle characteristics and environmental or toxicological endpoints. The views expressed in this poster are those of the authors and may not reflect U.S. EPA policy. The purpose of this submission for clearance is an abstract for submission to a scientific

Survey of EPA facilities for solar thermal energy applications

Science.gov (United States)

Nelson, E. V.; Overly, P. T.; Bell, D. M.

1980-01-01

A study was done to assess the feasibility of applying solar thermal energy systems to EPA facilities. A survey was conducted to determine those EPA facilities where solar energy could best be used. These systems were optimized for each specific application and the system/facility combinations were ranked on the basis of greatest cost effectiveness.

Abandoned Uranium Mines (AUM) Site Screening Map Service, 2016, US EPA Region 9

Data.gov (United States)

U.S. Environmental Protection Agency — As described in detail in the Five-Year Report, US EPA completed on-the-ground screening of 521 abandoned uranium mine areas. US EPA and the Navajo EPA are using the...

Using semantics for representing experimental protocols.

Science.gov (United States)

Giraldo, Olga; García, Alexander; López, Federico; Corcho, Oscar

2017-11-13

An experimental protocol is a sequence of tasks and operations executed to perform experimental research in biological and biomedical areas, e.g. biology, genetics, immunology, neurosciences, virology. Protocols often include references to equipment, reagents, descriptions of critical steps, troubleshooting and tips, as well as any other information that researchers deem important for facilitating the reusability of the protocol. Although experimental protocols are central to reproducibility, the descriptions are often cursory. There is the need for a unified framework with respect to the syntactic structure and the semantics for representing experimental protocols. In this paper we present "SMART Protocols ontology", an ontology for representing experimental protocols. Our ontology represents the protocol as a workflow with domain specific knowledge embedded within a document. We also present the S ample I nstrument R eagent O bjective (SIRO) model, which represents the minimal common information shared across experimental protocols. SIRO was conceived in the same realm as the Patient Intervention Comparison Outcome (PICO) model that supports search, retrieval and classification purposes in evidence based medicine. We evaluate our approach against a set of competency questions modeled as SPARQL queries and processed against a set of published and unpublished protocols modeled with the SP Ontology and the SIRO model. Our approach makes it possible to answer queries such as Which protocols use tumor tissue as a sample. Improving reporting structures for experimental protocols requires collective efforts from authors, peer reviewers, editors and funding bodies. The SP Ontology is a contribution towards this goal. We build upon previous experiences and bringing together the view of researchers managing protocols in their laboratory work. Website: https://smartprotocols.github.io/ .

Agriculture: About EPA's National Agriculture Center

Science.gov (United States)

EPA's National Agriculture Center (Ag Center), with the support of the United States Department of Agriculture, serves growers, livestock producers, other agribusinesses, and agricultural information/education providers.

GET SMART: EPA'S SMARTE INITIATIVE

Science.gov (United States)

The EPA's Office of Research and Development with the assistance of the U.S.-German Bilateral Working Group and the Interstate Technology Regulatory Council (ITRC), is developing Site-specific Management Approaches and Revitalization Tools (SMART) that will help stakeholders over...

EPA Principles for Greener Cleanups

Science.gov (United States)

A goal of the U.S. Environmental Protection Agency (EPA) Office and Land and Emergency Management (OLEM) and its many partners is to preserve and restore land by promoting and using protective waste management practices and by assessing and cleaning..

USDA-EPA Collaborative Ammonia Research

Science.gov (United States)

In 2014, a work group was formed between USDA and EPA to facilitate information exchange on ammonia emissions from agriculture, air quality impacts and emission mitigation options and to identify opportunities for collaboration. This document provides background on the work grou...

US EPA Digital Science: An Evolution

Science.gov (United States)

Ziegler, C. R.; Burch, K.; Laniak, G.; Vega, A.; Harten, P.; Kremer, J.; Brookes, A.; Yuen, A.; Subramanian, B.

2015-12-01

The United States Environmental Protection Agency's (US EPA) digital science "enterprise" plays a critical role in US EPA's efforts to achieve its mission to protect human health and the environment. This enterprise is an evolving cross-disciplinary research and development construct, with social and institutional dimensions. It has an active development community and produces a portfolio of digital science products including decision support tools, data repositories, Web interfaces, and more. Earth sciences and sustainable development organizations from around the world - including US government agencies - have achieved various levels of success in taking advantage of the rapidly-evolving digital age. Efficiency, transparency and ability to innovate are tied to an organization's digital maturity and related social characteristics. Concepts like participatory web, data and software interoperability, global technology transfer, ontological harmonization, big data, scaling, re-use and open science are no longer "new and emerging." They have emerged and - in some cases - are tied to US government directives. We assess maturity, describe future scenarios, discuss new initiatives and outline steps for better leveraging the information age to more effectively and efficiently achieve US EPA's mission. The views expressed herein are those of the authors and do not necessarily reflect the views or policies of the organizations for which they work and/or represent.

[Sampling, storage and transport of biological materials collected from living and deceased subjects for determination of concentration levels of ethyl alcohol and similarly acting substances. A proposal of updating the blood and urine sampling protocol].

Science.gov (United States)

Wiergowski, Marek; Reguła, Krystyna; Pieśniak, Dorota; Galer-Tatarowicz, Katarzyna; Szpiech, Beata; Jankowski, Zbigniew

2007-01-01

The present paper emphasizes the most common mistakes committed at the beginning of an analytical procedure. To shorten the time and decrease the cost of determinations of substances with similar to alcohol activity, it is postulated to introduce mass-scale screening analysis of saliva collected from a living subject at the site of the event, with all positive results confirmed in blood or urine samples. If no saliva sample is collected for toxicology, a urine sample, allowing for a stat fast screening analysis, and a blood sample, to confirm the result, should be ensured. Inappropriate storage of a blood sample in the tube without a preservative can cause sample spilling and its irretrievable loss. The authors propose updating the "Blood/urine sampling protocol", with the updated version to be introduced into practice following consultations and revisions.

EPA RE-Powering Mapper Region 7

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Large Scale

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Region 6

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Region 8

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Region 5

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Region 3

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Feasibility Studies

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Completed Installations

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Region 9

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Region 10

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Region 4

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Utility Scale

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Region 2

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA RE-Powering Mapper Region 1

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA Facility Registry Service (FRS): RCRA

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of hazardous waste...

EPA-Registered Repellents for Mosquitoes Transmitting Emerging Viral Disease.

Science.gov (United States)

Patel, Radha V; Shaeer, Kristy M; Patel, Pooja; Garmaza, Aleksey; Wiangkham, Kornwalee; Franks, Rachel B; Pane, Olivia; Carris, Nicholas W

2016-12-01

In many parts of the United States, mosquitoes were previously nuisance pests. However, they now represent a potential threat in the spread of viral diseases. The Aedes aegypti, Aedes albopictus, and Culex species mosquitoes are endemic to the United States and together may transmit a variety of viral diseases of growing concern, including West Nile virus, chikungunya, dengue fever, and Zika virus. The Centers for Disease Control and Prevention and the Environmental Protection Agency (EPA) recommend N,N-diethyl-meta-toluamide (DEET) as a first-line mosquito repellent, but for patients refusing to use DEET or other conventional repellents, guidance is limited to any EPA-registered product. Therefore, we conducted a systematic review of the literature to identify which EPA-registered personal mosquito repellent provides the best protection from A. aegypti, A. albopictus, and Culex spp. mosquitoes. We abstracted data from 62 published reports of EPA-registered mosquito repellents. The conventional repellent picaridin has the strongest data to support its use as a second-line agent, while IR3535 and oil of lemon eucalyptus are reasonably effective natural products. Citronella, catnip, and 2-undecanone offer limited protection or have limited data. These results can be used by pharmacists and other health care professionals to advise patients on the selection of an EPA-registered mosquito repellent. Regardless of the repellent chosen, it is vital for patients to follow all instructions/precautions in the product labeling to ensure safe and effective use. © 2016 Pharmacotherapy Publications, Inc.

The role of multispectral scanners as data sources for EPA hydrologic models

Science.gov (United States)

Slack, R.; Hill, D.

1982-01-01

An estimated cost savings of 30% to 50% was realized from using LANDSAT-derived data as input into a program which simulates hydrologic and water quality processes in natural and man-made water systems. Data from the satellite were used in conjunction with EPA's 11-channel multispectral scanner to obtain maps for characterizing the distribution of turbidity plumes in Flathead Lake and to predict the effect of increasing urbanization in Montana's Flathead River Basin on the lake's trophic state. Multispectral data are also being studied as a possible source of the parameters needed to model the buffering capability of lakes in an effort to evaluate the effect of acid rain in the Adirondacks. Water quality in Lake Champlain, Vermont is being classified using data from the LANDSAT and the EPA MSS. Both contact-sensed and MSS data are being used with multivariate statistical analysis to classify the trophic status of 145 lakes in Illinois and to identify water sampling sites in Appalachicola Bay where contaminants threaten Florida's shellfish.

EPA's Benchmark Dose Modeling Software

Science.gov (United States)

The EPA developed the Benchmark Dose Software (BMDS) as a tool to help Agency risk assessors facilitate applying benchmark dose (BMD) method’s to EPA’s human health risk assessment (HHRA) documents. The application of BMD methods overcomes many well know limitations ...

EPA Facility Registry Service (FRS): NCDB

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): BRAC

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): BIA

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): RADINFO

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): TRI

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): ICIS

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): RBLC

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry System (FRS): NCES

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): LANDFILL

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of non-hazardous waste...

EPA Facility Registry Service (FRS): NEI

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): CAMDBS

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry System (FRS): NEPT

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): SDWIS

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): RMP

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

Green Roof Research through EPA's Regional Applied Research Effort - slides

Science.gov (United States)

The U.S. Environmental Protection Agency’s (EPA) Regional Applied Research Effort (RARE) allows the Regions of the EPA to choose research projects to be performed in partnership with EPA’s Office of Research and Development (ORD). Over the last decade, several green roof projects...

40 CFR 70.8 - Permit review by EPA and affected States.

Science.gov (United States)

2010-07-01

... compatible with EPA's national database management system. (2) The Administrator may waive the requirements... PROGRAMS (CONTINUED) STATE OPERATING PERMIT PROGRAMS § 70.8 Permit review by EPA and affected States. (a...

Verbal protocols as methodological resources: research evidence

Directory of Open Access Journals (Sweden)

Alessandra Baldo

2012-01-01

Full Text Available This article aims at reflecting on the use of verbal protocols as a methodological resource in qualitative research, more specifically on the aspect regarded as the main limitation of a study about lexical inferencing in L2 (BALDO; VELASQUES, 2010: its subjective trait. The article begins with a brief literature review on protocols, followed by a description of the study in which they were employed as methodological resources. Based on that, protocol subjectivity is illustrated through samples of unparalleled data classification, carried out independently by two researchers. In the final section, the path followed to minimize the problem is presented, intending to contribute to improve efficiency in the use of verbal protocols in future research.

EPA Pacific Southwest Enforcement Division Inspected Tax Map Key Polygons, Hawaii, 2017, US EPA Region 9

Data.gov (United States)

U.S. Environmental Protection Agency — This feature class contains the 64 tax map key polygons across the state of Hawaii that have been inspected by US EPA Pacific Southwest Enforcement Division as of...

EPA/DOE joint efforts on mixed waste treatment

International Nuclear Information System (INIS)

Lee, C.C.; Huffman, G.L.; Nalesnik, R.P.

1995-01-01

Under the requirements of the Federal Facility Compliance Act (FFCA), the Department of Energy (DOE) is directed to develop treatment plans for their stockpile of wastes generated at their various sites. As a result, DOE is facing the monumental problem associated with the treatment and ultimate disposal of their mixed (radioactive and hazardous) waste. Meanwhile, the Environmental Protection Agency (EPA) issued a final open-quotes Hazardous Waste Combustion Strategyclose quotes in November 1994. Under the Combustion Strategy, EPA permit writers have been given the authority to use the Omnibus Provision of the Resource Conservation and Recovery Act (RCRA) to impose more stringent emission limits for waste combustors prior to the development of new regulations. EPA and DOE established a multi-year Interagency Agreement (IAG) in 1991. The main objective of the IAG (and of the second IAG that was added in 1993) is to conduct a research program on thermal technologies for treating mixed waste and to establish permit procedures for these technologies particularly under the new requirements of the above-mentioned EPA Combustion Strategy. The objective of this Paper is to summarize the results of the EPA/DOE joint efforts on mixed waste treatment since the establishment of the original Interagency Agreement. Specifically, this Paper will discuss six activities that have been underway; namely: (1) National Technical Workgroup (NTW) on Mixed Waste Treatment, (2) State-of-the-Art Assessment of APC (Air Pollution Control) and Monitoring Technologies for the Rocky Flats Fluidized Bed Unit, (3) Initial Study of Permit open-quotes Roadmapclose quotes Development for Mixed Waste Treatment, (4) Risk Assessment Approach for a Mixed Waste Thermal Treatment Facility, (5) Development and Application of Technology Selection Criteria for Mixed Waste Thermal Treatment, and (6) Performance Testing of Mixed Waste Incineration: In-Situ Chlorine Capture in a Fluidized Bed Unit

Comparability between NQA-1 and the QA programs for analytical laboratories within the nuclear industry and EPA hazardous waste laboratories

International Nuclear Information System (INIS)

English, S.L.; Dahl, D.R.

1989-01-01

There is increasing cooperation between the Department of Energy (DOE), Department of Defense (DOD), and the Environmental Protection Agency (EPA) in the activities associated with monitoring and clean-up of hazardous wastes. Pacific Northwest Laboratory (PNL) examined the quality assurance/quality control programs that the EPA requires of the private sector when performing routine analyses of hazardous wastes to confirm how or if the requirements correspond with PNL's QA program based upon NQA-1. This paper presents the similarities and differences between NQA-1 and the QA program identified in ASTM-C1009-83, Establishing a QA Program for Analytical Chemistry Laboratories within the Nuclear Industry; EPA QAMS-005/80, Interim Guidelines and Specifications for Preparing Quality Assurance Project Plans, which is referenced in Statements of Work for CERCLA analytical activities; and Chapter 1 of SW-846, which is used in analyses of RCRA samples. The EPA QA programs for hazardous waste analyses are easily encompassed within an already established NQA-1 QA program. A few new terms are introduced and there is an increased emphasis upon the QC/verification, but there are many of the same basic concepts in all the programs

Concerns raised over new EPA members

Science.gov (United States)

Gwynne, Peter

2017-12-01

The Trump administration has nominated three new members of the Environmental Protection Agency (EPA) who critics say are undermining laws and “pampering” the industries they are supposed to regulate.

EPA Facility Registry Service (FRS): OIL

Data.gov (United States)

U.S. Environmental Protection Agency — This dataset contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link to the Oil...

Technical Note: Comparison of storage strategies of sea surface microlayer samples

Directory of Open Access Journals (Sweden)

K. Schneider-Zapp

2013-07-01

Full Text Available The sea surface microlayer (SML is an important biogeochemical system whose physico-chemical analysis often necessitates some degree of sample storage. However, many SML components degrade with time so the development of optimal storage protocols is paramount. We here briefly review some commonly used treatment and storage protocols. Using freshwater and saline SML samples from a river estuary, we investigated temporal changes in surfactant activity (SA and the absorbance and fluorescence of chromophoric dissolved organic matter (CDOM over four weeks, following selected sample treatment and storage protocols. Some variability in the effectiveness of individual protocols most likely reflects sample provenance. None of the various protocols examined performed any better than dark storage at 4 °C without pre-treatment. We therefore recommend storing samples refrigerated in the dark.

Hispanos en la EPA: Neftalí Hernández-Santiago

Science.gov (United States)

La diversidad de la fuerza laboral es importante para la Agencia de Protección Ambiental de EE.UU. (EPA, por sus siglas en inglés). Los empleados hispanos de la EPA contribuyen diariamente hacia la protección de la salud y el medio ambiente.

Sample preparation guidelines for two-dimensional electrophoresis.

Science.gov (United States)

Posch, Anton

2014-12-01

Sample preparation is one of the key technologies for successful two-dimensional electrophoresis (2DE). Due to the great diversity of protein sample types and sources, no single sample preparation method works with all proteins; for any sample the optimum procedure must be determined empirically. This review is meant to provide a broad overview of the most important principles in sample preparation in order to avoid a multitude of possible pitfalls. Sample preparation protocols from the expert in the field were screened and evaluated. On the basis of these protocols and my own comprehensive practical experience important guidelines are given in this review. The presented guidelines will facilitate straightforward protocol development for researchers new to gel-based proteomics. In addition the available choices are rationalized in order to successfully prepare a protein sample for 2DE separations. The strategies described here are not limited to 2DE and can also be applied to other protein separation techniques.

78 FR 13872 - Proposed Information Collection Request; Comment Request; EPA's WaterSense Program (Renewal); EPA...

Science.gov (United States)

2013-03-01

... profanity, threats, information claimed to be Confidential Business Information (CBI) or other information...-efficient products and use water-efficient practices. As part of strategic planning efforts, EPA encourages... Reporting Form Promotional partners 6100-09 Manufacturers (separate forms for plumbing and non- plumbing...

EPA Center for Corporate Climate Leadership

Science.gov (United States)

EPA's Center for Corporate Climate Leadership is a comprehensive resource to help organizations measure & manage GHG emissions. The Center provides technical tools, educational resources, opportunities for information sharing & highlights best practices.

EPA CIO Governance Board Membership List

Science.gov (United States)

In keeping with OMB guidance on implementing the Federal Information Technology Reform Act (FITARA), EPA is publishing its list of officials who perform the duties or responsibilities of a Bureau CIO.

EPA Administrative Law Judge Legal Documents

Data.gov (United States)

U.S. Environmental Protection Agency — This dataset contains Decisions and Orders originating from EPAs Office of Administrative Law Judges (OALJ), which is an independent office in the Office of the...

2013 EPA Vessels General Permit (VGP)

Data.gov (United States)

U.S. Environmental Protection Agency — Information for any vessel that submitted a Notice of Intent (NOI), Notice of Termination (NOT), or annual report under EPA's 2013 Vessel General Permit (VGP)....

EPA Facility Registry Service (FRS): ACRES

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service consists of location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of sites that link to...

Evaluation of mercury speciation by EPA (Draft) Method 29

Energy Technology Data Exchange (ETDEWEB)

Laudal, D.L.; Heidt, M.K. [Energy & Environmental Research Center, Grand Forks, ND (United States); Nott, B. [Electric Power Research Institute, Palo Alto, CA (United States)

1995-11-01

The 1990 Clean Air Act Amendments require that the U.S. Environmental protection Agency (EPA) assess the health risks associated with mercury emissions. Also, the law requires a separate assessment of health risks posed by the emission of 189 tract chemicals (including mercury) for electric utility steam-generating units. In order to conduct a meaningful assessment of health and environmental effects, we must have, among other things, a reliable and accurate method to measure mercury emissions. In addition, the rate of mercury deposition and the type of control strategies used may depend upon the type of mercury emitted (i.e., whether it is in the oxidized or elemental form). It has been speculated that EPA (Draft) Method 29 can speciate mercury by selective absorption; however, this claim has yet to be proven. The Electric Power Research Institute (EPRI) and the U.S. Department of Energy (DOE) have contracted with the Energy & Environmental Research Center (EERC) at University of North Dakota to evaluate EPA (Draft) Method 29 at the pilot-scale level. The objective of the work is to determine whether EPA (Draft) Method 29 can reliably quantify and speciate mercury in the flue gas from coal-fired boilers.

Scenario sensitivity analyses performed on the PRESTO-EPA LLW risk assessment models

International Nuclear Information System (INIS)

Bandrowski, M.S.

1988-01-01

The US Environmental Protection Agency (EPA) is currently developing standards for the land disposal of low-level radioactive waste. As part of the standard development, EPA has performed risk assessments using the PRESTO-EPA codes. A program of sensitivity analysis was conducted on the PRESTO-EPA codes, consisting of single parameter sensitivity analysis and scenario sensitivity analysis. The results of the single parameter sensitivity analysis were discussed at the 1987 DOE LLW Management Conference. Specific scenario sensitivity analyses have been completed and evaluated. Scenario assumptions that were analyzed include: site location, disposal method, form of waste, waste volume, analysis time horizon, critical radionuclides, use of buffer zones, and global health effects

Protocol voor meting van lachgasemissie uit huisvestingssystemen in de veehouderij 2010 = Measurement protocol for nitrous oxide emission from housing systems in livestock production 2010

NARCIS (Netherlands)

Mosquera Losada, J.; Groenestein, C.M.; Ogink, N.W.M.

2011-01-01

This report describes a measurement protocol for nitrous oxide emissions from animal housing systems. The protocol is based on sampling periods of 24 hours spread over one year and can be applied in specified animal categories.

48 CFR 1552.223-71 - EPA Green Meetings and Conferences.

Science.gov (United States)

2010-10-01

... provision or language substantially the same as the provision in solicitations for meetings and conference services. EPA Green Meetings and Conferences (MAY 2007) (a) The mission of the EPA is to protect human... information about environmentally preferable features and practices your facility will have in place for the...

Meet EPA Researcher Endalkachew Sahle-Demessie

Science.gov (United States)

Meet EPA Researcher Endalkachew Sahle-Demessie. Chemical and Environmental Engineer Endalkachew Sahle-Demessie, Ph.D., works on various projects, including nanomaterials and water resources, in EPA’s National Risk Management Research Laboratory.

EPA Region 1 Sole Source Aquifers

Data.gov (United States)

U.S. Environmental Protection Agency — This coverage contains boundaries of EPA-approved sole source aquifers. Sole source aquifers are defined as an aquifer designated as the sole or principal source of...

Electrostatic Plasma Accelerator (EPA)

Science.gov (United States)

Brophy, John R.; Aston, Graeme

1995-01-01

The application of electric propulsion to communications satellites, however, has been limited to the use of hydrazine thrusters with electric heaters for thrust and specific impulse augmentation. These electrothermal thrusters operate at specific impulse levels of approximately 300 s with heater powers of about 500 W. Low power arcjets (1-3 kW) are currently being investigated as a way to increase specific impulse levels to approximately 500 s. Ion propulsion systems can easily produce specific impulses of 3000 s or greater, but have yet to be applied to communications satellites. The reasons most often given for not using ion propulsion systems are their high level of overall complexity, low thrust with long burn times, and the difficulty of integrating the propulsion system into existing commercial spacecraft busses. The Electrostatic Plasma Accelerator (EPA) is a thruster concept which promises specific impulse levels between low power arcjets and those of the ion engine while retaining the relative simplicity of the arcjet. The EPA thruster produces thrust through the electrostatic acceleration of a moderately dense plasma. No accelerating electrodes are used and the specific impulse is a direct function of the applied discharge voltage and the propellant atomic mass.

Comments on EPA's LLW preproposal

International Nuclear Information System (INIS)

Littleton, B.K.; Weinstock, L.

1995-01-01

The Environmental Protection Agency (EPA) is currently developing standards for the management, storage, and disposal of Low-Level Radioactive Waste (LLW). The Atomic Energy Act delegated EPA, among other provisions, the authority to establish generally applicable standards for the disposal of radioactive waste to ensure that the public and the environment are adequately protected from potential radiation impacts. As an initial effort to open communications on a standard for LLW, the Agency developed a preproposal draft (Preproposal Draft of 40 CFR Part 193 - 30 Nov 94) and circulated it to interested parties for review and comment. The extended comment period ended April 12, 1995. A summary of the comments received and analyzed to date follows. After all comments have been analyzed, the rule will undergo an Agency clearance process and be sent to the Office of Management and Budget for review. After that review, the formal process of publication of the proposed rule in the Federal Register and the formal public comment period will begin

Environmental Protection Agency - EPA Pub Central

Data.gov (United States)

U.S. Environmental Protection Agency — PubMed Central (PMC) is a full-text, online archive of journal literature operated by the National Library of Medicine. The EPA is using PMC to permanently preserve...

Continuous sampling from distributed streams

DEFF Research Database (Denmark)

Graham, Cormode; Muthukrishnan, S.; Yi, Ke

2012-01-01

A fundamental problem in data management is to draw and maintain a sample of a large data set, for approximate query answering, selectivity estimation, and query planning. With large, streaming data sets, this problem becomes particularly difficult when the data is shared across multiple distribu......A fundamental problem in data management is to draw and maintain a sample of a large data set, for approximate query answering, selectivity estimation, and query planning. With large, streaming data sets, this problem becomes particularly difficult when the data is shared across multiple...... distributed sites. The main challenge is to ensure that a sample is drawn uniformly across the union of the data while minimizing the communication needed to run the protocol on the evolving data. At the same time, it is also necessary to make the protocol lightweight, by keeping the space and time costs low...... for each participant. In this article, we present communication-efficient protocols for continuously maintaining a sample (both with and without replacement) from k distributed streams. These apply to the case when we want a sample from the full streams, and to the sliding window cases of only the W most...

Improving Sampling, Analysis, and Data Management for Site Investigation and Cleanup

Science.gov (United States)

The United States Environmental Protection Agency (EPA) supports the adoption of streamlined approaches to sampling, analysis, and data management activities conducted during site assessment, characterization, and cleanup.

40 CFR 131.22 - EPA promulgation of water quality standards.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 21 2010-07-01 2010-07-01 false EPA promulgation of water quality... PROGRAMS WATER QUALITY STANDARDS Procedures for Review and Revision of Water Quality Standards § 131.22 EPA promulgation of water quality standards. (a) If the State does not adopt the changes specified by the Regional...

76 FR 27315 - EPA Science Advisory Board Staff Invitation to a Session on Public Involvement in EPA Advisory...

Science.gov (United States)

2011-05-11

... and SAB Three inter-related public access Web sites ( http://www.epa.gov/advisorycouncilcaa , http...) ``Recent Happenings'' and ``Recent Additions'' sections and related ``really simple syndication'' (RSS...

Regulatory decision with EPA/NRC/DOE/State Session (Panel)

Energy Technology Data Exchange (ETDEWEB)

O`Donnell, E.

1995-12-31

This panel will cover the Nuclear Regulatory Commission`s (NRC) proposed radiation limits in the Branch Technical Position on Low-Level Radioactive Waste Performance Assessment and the Environmental Protection Agency`s (EPA) draft regulation in Part 193. Representatives from NRC and EPA will discuss the inconsistencies in these two regulations. DOE and state representatives will discuss their perspective on how these regulations will affect low-level radioactive waste performance assessments.

Comparison of Enterococcus quantitative polymerase chain reaction analysis results from midwest U.S. river samples using EPA Method 1611 and Method 1609 PCR reagents

Science.gov (United States)

The U.S. Environmental Protection Agency (EPA) has provided recommended beach advisory values in its 2012 recreational water quality criteria (RWQC) for states wishing to use quantitative polymerase chain reaction (qPCR) for the monitoring of Enterococcus fecal indicator bacteria...

A distance limited method for sampling downed coarse woody debris

Science.gov (United States)

Jeffrey H. Gove; Mark J. Ducey; Harry T. Valentine; Michael S. Williams

2012-01-01

A new sampling method for down coarse woody debris is proposed based on limiting the perpendicular distance from individual pieces to a randomly chosen sample point. Two approaches are presented that allow different protocols to be used to determine field measurements; estimators for each protocol are also developed. Both protocols are compared via simulation against...

Single point aerosol sampling: Evaluation of mixing and probe performance in a nuclear stack

Energy Technology Data Exchange (ETDEWEB)

Rodgers, J.C.; Fairchild, C.I.; Wood, G.O. [Los Alamos National Laboratory, NM (United States)] [and others

1995-02-01

Alternative Reference Methodologies (ARMs) have been developed for sampling of radionuclides from stacks and ducts that differ from the methods required by the U.S. EPA. The EPA methods are prescriptive in selection of sampling locations and in design of sampling probes whereas the alternative methods are performance driven. Tests were conducted in a stack at Los Alamos National Laboratory to demonstrate the efficacy of the ARMs. Coefficients of variation of the velocity tracer gas, and aerosol particle profiles were determined at three sampling locations. Results showed numerical criteria placed upon the coefficients of variation by the ARMs were met at sampling stations located 9 and 14 stack diameters from flow entrance, but not at a location that is 1.5 diameters downstream from the inlet. Experiments were conducted to characterize the transmission of 10 {mu}m aerodynamic equivalent diameter liquid aerosol particles through three types of sampling probes. The transmission ratio (ratio of aerosol concentration at the probe exit plane to the concentration in the free stream) was 107% for a 113 L/min (4-cfm) anisokinetic shrouded probe, but only 20% for an isokinetic probe that follows the EPA requirements. A specially designed isokinetic probe showed a transmission ratio of 63%. The shrouded probe performance would conform to the ARM criteria; however, the isokinetic probes would not.

Soil Gas Sample Handling: Evaluation of Water Removal and Sample Ganging

Energy Technology Data Exchange (ETDEWEB)

Fritz, Brad G. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Abrecht, David G. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hayes, James C. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Mendoza, Donaldo P. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

2016-10-31

Soil gas sampling is currently conducted in support of Nuclear Test Ban treaty verification. Soil gas samples are collected and analyzed for isotopes of interest. Some issues that can impact sampling and analysis of these samples are excess moisture and sample processing time. Here we discuss three potential improvements to the current sampling protocol; a desiccant for water removal, use of molecular sieve to remove CO₂ from the sample during collection, and a ganging manifold to allow composite analysis of multiple samples.

77 FR 46089 - Agency Information Collection Activities; Proposed Collection; Comment Request; EPA's ENERGY STAR...

Science.gov (United States)

2012-08-02

... Activities; Proposed Collection; Comment Request; EPA's ENERGY STAR Program in the Commercial and Industrial... this action are participants in EPA's ENERGY STAR Program in the Commercial and Industrial Sectors. Title: Information Collection Activities Associated with EPA's ENERGY STAR Program in the Commercial and...

40 CFR 725.17 - Consultation with EPA.

Science.gov (United States)

2010-07-01

... ACT REPORTING REQUIREMENTS AND REVIEW PROCESSES FOR MICROORGANISMS General Provisions and..., ATTN: Biotechnology Notice Consultation. Persons wishing to consult with EPA by telephone should call...

EPA Communications Stylebook: Training and Education

Science.gov (United States)

It is the policy of EPA that our staff should have and develop good communications skills. Besides writing, style, and design skills, we seek to develop audience analysis and targeting, marketing and media selection, and computer skills.

NIEHS/EPA Children's Environmental Health and Disease Prevention Research Centers: 2017 Annual Meeting Proceedings

Science.gov (United States)

The 2017 Annual Meeting of the NIEHS/EPA Children’s Environmental Health and Disease Prevention Research Centers was hosted by EPA in collaboration with NIEHS and the Pediatric Environmental Health Specialty Units (PEHSUs). The meeting was held at the EPA Region 9 offices i...

Dietary Intakes of EPA and DHA Omega-3 Fatty Acids among US Childbearing-Age and Pregnant Women: An Analysis of NHANES 2001-2014.

Science.gov (United States)

Zhang, Zhiying; Fulgoni, Victor L; Kris-Etherton, Penny M; Mitmesser, Susan Hazels

2018-03-28

The 2015–2020 Dietary Guidelines for Americans (DGA) recommend that the general population should consume about 8 ounces (oz.) per week of a variety of seafood, providing approximately 250 mg per day of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and that pregnant and lactating women should consume 8–12 oz. per week of seafood. We determined the usual intakes, percentage not meeting recommendations, and trends in EPA and DHA intakes among childbearing-age and pregnant women (15–44 years of age) using the NHANES cycles 2001–2002 through 2013–2014. For the childbearing-age women, the mean usual intake of seafood was 0.44 ± 0.02 oz. equivalent per day and 100% of the population was below the DGA recommendation. Mean usual intakes of EPA, DHA, and combined EPA and DHA from foods and dietary supplements combined were 26.8 ± 1.4, 62.2 ± 1.9, and 88.1 ± 3.0 mg per day, respectively. Over 95% of the sample did not meet the daily intakes of 250 mg EPA and DHA. Similar results were observed for pregnant women. After controlling for covariates, there were slight but significant increases in EPA and DHA intakes from foods and dietary supplements over the 14-year span among childbearing-age ( p = 0.005) and pregnant women ( p = 0.002). It was estimated that a majority of U.S. childbearing-age and pregnant women consumed significantly lower amounts of seafood than what the DGA recommends, which subsequently leads to low intakes of EPA and DHA; in addition, dietary supplement use has not eliminated the nutrient shortfall.

EPA Region 1 Environmentally Sensitive Areas (Points)

Data.gov (United States)

U.S. Environmental Protection Agency — This coverage represents point equivalents of environmentally sensitive areas in EPA New England. This coverage represents polygon equivalents of environmentally...

ISS protocol for EPR tooth dosimetry

International Nuclear Information System (INIS)

Onori, S.; Aragno, D.; Fattibene, P.; Petetti, E.; Pressello, M.C.

2000-01-01

The accuracy in Electron Paramagnetic Resonance (EPR) dose reconstruction with tooth enamel is affected by sample preparation, dosimetric signal amplitude evaluation and unknown dose estimate. Worldwide efforts in the field of EPR dose reconstruction with tooth enamel are focused on the optimization of the three mentioned steps in dose assessment. In the present work, the protocol implemented at ISS in the framework of the European Community Nuclear Fission Safety project 'Dose Reconstruction' is presented. A combined mechanical-chemical procedure for ground enamel sample preparation is used. The signal intensity evaluation is carried out with powder spectra simulation program. Finally, the unknown dose is evaluated individually for each sample with the additive dose method. The unknown dose is obtained by subtracting a mean native dose from the back-extrapolated dose. As an example of the capability of the ISS protocol in unknown dose evaluation, the results obtained in the framework of the 2nd International Intercomparison on EPR tooth enamel dosimetry are reported

Protocol for Measuring the Thermal Properties of a Supercooled Synthetic Sand-water-gas-methane Hydrate Sample.

Science.gov (United States)

Muraoka, Michihiro; Susuki, Naoko; Yamaguchi, Hiroko; Tsuji, Tomoya; Yamamoto, Yoshitaka

2016-03-21

Methane hydrates (MHs) are present in large amounts in the ocean floor and permafrost regions. Methane and hydrogen hydrates are being studied as future energy resources and energy storage media. To develop a method for gas production from natural MH-bearing sediments and hydrate-based technologies, it is imperative to understand the thermal properties of gas hydrates. The thermal properties' measurements of samples comprising sand, water, methane, and MH are difficult because the melting heat of MH may affect the measurements. To solve this problem, we performed thermal properties' measurements at supercooled conditions during MH formation. The measurement protocol, calculation method of the saturation change, and tips for thermal constants' analysis of the sample using transient plane source techniques are described here. The effect of the formation heat of MH on measurement is very small because the gas hydrate formation rate is very slow. This measurement method can be applied to the thermal properties of the gas hydrate-water-guest gas system, which contains hydrogen, CO2, and ozone hydrates, because the characteristic low formation rate of gas hydrate is not unique to MH. The key point of this method is the low rate of phase transition of the target material. Hence, this method may be applied to other materials having low phase-transition rates.

EPA Region 1 - Map Layers for Valley ID Tool (Hosted Feature Service)

Data.gov (United States)

U.S. Environmental Protection Agency — The Valley Service Feature Layer hosts spatial data for EPA Region 1's Valley Identification Tool. These layers contain attribute information added by EPA R1 GIS...

Procedures for EPA Libraries to Obtain Materials through Interlibrary Loan/Document Delivery

Science.gov (United States)

The purpose of this document is to establish Agency-wide procedures by which EPA libraries obtain materials for Agency employees and authorized EPA contractors through interlibrary loan (ILL) and other document delivery methods.

48 CFR 1552.203-71 - Display of EPA Office of Inspector General Hotline poster.

Science.gov (United States)

2010-10-01

... Inspector General Hotline poster. 1552.203-71 Section 1552.203-71 Federal Acquisition Regulations System... Provisions and Clauses 1552.203-71 Display of EPA Office of Inspector General Hotline poster. As prescribed... all contract options. Display of EPA Office of Inspector General Hotline Poster (AUG 2000) (a) For EPA...

48 CFR 1552.219-71 - Procedures for Participation in the EPA Mentor-Protege Program.

Science.gov (United States)

2010-10-01

... Participation in the EPA Mentor-Protege Program. 1552.219-71 Section 1552.219-71 Federal Acquisition Regulations... Texts of Provisions and Clauses 1552.219-71 Procedures for Participation in the EPA Mentor-Protege... EPA Mentor-Protege Program (OCT 2000) (a) This provision sets forth the procedures for participation...

76 FR 42707 - Amendment of OIG Hotline Allegation System (EPA-30)

Science.gov (United States)

2011-07-19

... . The http://www.regulations.gov Web site is an ``anonymous access'' system, which means EPA will not... docket and made available on the Internet. If you submit an electronic comment, EPA recommends that you... the complainant (except for anonymous complainants), date complaint received, program area, nature and...

Impacts of the Japan-Mexico EPA on Bilateral Trade

OpenAIRE

ANDO Mitsuyo; URATA Shujiro

2011-01-01

This paper examines the impacts of the Japan-Mexico EPA on bilateral trade by using two different types of information, trade statistics and the EPA utilization rate. Using trade data, we found that Japan's exports of built-up cars, auto parts, base metals, electrical machinery, precision machinery, and ballpoint pens to Mexico increased sharply. We also found that Japan's imports of live animals and products, leather, and footwear with leather from Mexico increased significantly. These are s...

INVESTIGATION OF THE TOTAL ORGANIC HALOGEN ANALYTICAL METHOD AT THE WASTE SAMPLING CHARACTERIZATION FACILITY (WSCF)

International Nuclear Information System (INIS)

DOUGLAS JG; MEZNARICH HD, PHD; OLSEN JR; ROSS GA; STAUFFER M

2008-01-01

Total organic halogen (TOX) is used as a parameter to screen groundwater samples at the Hanford Site. Trending is done for each groundwater well, and changes in TOX and other screening parameters can lead to costly changes in the monitoring protocol. The Waste Sampling and Characterization Facility (WSCF) analyzes groundwater samples for TOX using the United States Environmental Protection Agency (EPA) SW-846 method 9020B (EPA 1996a). Samples from the Soil and Groundwater Remediation Project (S and GRP) are submitted to the WSCF for analysis without information regarding the source of the sample; each sample is in essence a 'blind' sample to the laboratory. Feedback from the S and GRP indicated that some of the WSCF-generated TOX data from groundwater wells had a number of outlier values based on the historical trends (Anastos 2008a). Additionally, analysts at WSCF observed inconsistent TOX results among field sample replicates. Therefore, the WSCF lab performed an investigation of the TOX analysis to determine the cause of the outlier data points. Two causes were found that contributed to generating out-of-trend TOX data: (1) The presence of inorganic chloride in the groundwater samples: at inorganic chloride concentrations greater than about 10 parts per million (ppm), apparent TOX values increase with increasing chloride concentration. A parallel observation is the increase in apparent breakthrough of TOX from the first to the second activated-carbon adsorption tubes with increasing inorganic chloride concentration. (2) During the sample preparation step, excessive purging of the adsorption tubes with oxygen pressurization gas after sample loading may cause channeling in the activated-carbon bed. This channeling leads to poor removal of inorganic chloride during the subsequent wash step with aqueous potassium nitrate. The presence of this residual inorganic chloride then produces erroneously high TOX values. Changes in sample preparation were studied to more

INVESTIGATION OF THE TOTAL ORGANIC HALOGEN ANALYTICAL METHOD AT THE WASTE SAMPLING AND CHARACTERIZATION FACILITY

International Nuclear Information System (INIS)

Douglas, J.G.; Meznarich, H.K.; Olsen, J.R.; Ross, G.A.; Stauffer, M.

2009-01-01

Total organic halogen (TOX) is used as a parameter to screen groundwater samples at the Hanford Site. Trending is done for each groundwater well, and changes in TOX and other screening parameters can lead to costly changes in the monitoring protocol. The Waste Sampling and Characterization Facility (WSCF) analyzes groundwater samples for TOX using the United States Environmental Protection Agency (EPA) SW-S46 method 9020B (EPA 1996a). Samples from the Soil and Groundwater Remediation Project (SGRP) are submitted to the WSCF for analysis without information regarding the source of the sample; each sample is in essence a ''blind'' sample to the laboratory. Feedback from the SGRP indicated that some of the WSCF-generated TOX data from groundwater wells had a number of outlier values based on the historical trends (Anastos 200Sa). Additionally, analysts at WSCF observed inconsistent TOX results among field sample replicates. Therefore, the WSCF lab performed an investigation of the TOX analysis to determine the cause of the outlier data points. Two causes were found that contributed to generating out-of-trend TOX data: (1) The presence of inorganic chloride in the groundwater samples: at inorganic chloride concentrations greater than about 10 parts per million (ppm), apparent TOX values increase with increasing chloride concentration. A parallel observation is the increase in apparent breakthrough of TOX from the first to the second activated-carbon adsorption tubes with increasing inorganic chloride concentration. (2) During the sample preparation step, excessive purging of the adsorption tubes with oxygen pressurization gas after sample loading may cause channeling in the activated carbon bed. This channeling leads to poor removal of inorganic chloride during the subsequent wash step with aqueous potassium nitrate. The presence of this residual inorganic chloride then produces erroneously high TOX values. Changes in sample preparation were studied to more effectively

EPA RE-Powering Mapper Solar on Landfills

Data.gov (United States)

U.S. Environmental Protection Agency — The U.S. Environmental Protection Agency (EPA) Office of Land and Emergency Management (OLEM) Office of Communications, Partnerships and Analysis (OCPA) initiated...

EPA Facility Registry Service (FRS): RCRA_INACTIVE

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of hazardous waste...

EPA Facility Registry Service (FRS): RCRA_ACTIVE

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of active hazardous...

EPA Linked Open Data: Facility Registry Service

Data.gov (United States)

U.S. Environmental Protection Agency — The Facility Registry Service (FRS) identifies facilities, sites, or places subject to environmental regulation or of environmental interest to EPA programs or...

EPA Facility Registry Service (FRS): RCRA_TSD

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of Hazardous Waste...

U.S. EPAs Geospatial Data Access Project

Data.gov (United States)

U.S. Environmental Protection Agency — To improve public health and the environment, the United States Environmental Protection Agency (EPA) collects information about facilities, sites, or places subject...

Registry of EPA Applications, Models, and Databases

Data.gov (United States)

U.S. Environmental Protection Agency — READ is EPA's authoritative source for information about Agency information resources, including applications/systems, datasets and models. READ is one component of...

EMS Adherence to a Pre-hospital Cervical Spine Clearance Protocol

Directory of Open Access Journals (Sweden)

Johnson, David

2001-10-01

Full Text Available Purpose: To determine the degree of adherence to a cervical spine (c-spine clearance protocol by pre-hospital Emergency Medical Services (EMS personnel by both self-assessment and receiving hospital assessment, to describe deviations from the protocol, and to determine if the rate of compliance by paramedic self-assessment differed from receiving hospital assessment. Methods: A retrospective sample of pre-hospital (consecutive series and receiving hospital (convenience sample assessments of the compliance with and appropriateness of c-spine immobilization. The c-spine clearance protocol was implemented for Orange County EMS just prior to the April-November 1999 data collection period. Results: We collected 396 pre-hospital and 162 receiving hospital data forms. From the pre-hospital data sheet. the percentage deviation from the protocol was 4.096 (16/396. Only one out of 16 cases that did not comply with the protocol was due to over immobilization (0.2%. The remaining 15 cases were under immobilized, according to protocol. Nine of the under immobilized cases (66% that should have been placed in c-spine precautions met physical assessment criteria in the protocol, while the other five cases met mechanism of injury criteria. The rate of deviations from protocol did not differ over time. The receiving hospital identified 8.0% (13/162; 6/16 over immobilized, 7/16 under immobilized of patients with deviations from the protocol; none was determined to have actual c-spine injury. Conclusion: The implementation of a pre-hospital c-spine clearance protocol in Orange County was associated with a moderate overall adherence rate (96% from the pre-hospital perspective, and 92% from the hospital perspective, p=.08 for the two evaluation methods. Most patients who deviated from protocol were under immobilized, but no c-spine injuries were missed. The rate of over immobilization was better than previously reported, implying a saving of resources.

An optimised protocol for molecular identification of Eimeria from chickens☆

Science.gov (United States)

Kumar, Saroj; Garg, Rajat; Moftah, Abdalgader; Clark, Emily L.; Macdonald, Sarah E.; Chaudhry, Abdul S.; Sparagano, Olivier; Banerjee, Partha S.; Kundu, Krishnendu; Tomley, Fiona M.; Blake, Damer P.

2014-01-01

Molecular approaches supporting identification of Eimeria parasites infecting chickens have been available for more than 20 years, although they have largely failed to replace traditional measures such as microscopy and pathology. Limitations of microscopy-led diagnostics, including a requirement for specialist parasitological expertise and low sample throughput, are yet to be outweighed by the difficulties associated with accessing genomic DNA from environmental Eimeria samples. A key step towards the use of Eimeria species-specific PCR as a sensitive and reproducible discriminatory tool for use in the field is the production of a standardised protocol that includes sample collection and DNA template preparation, as well as primer selection from the numerous PCR assays now published. Such a protocol will facilitate development of valuable epidemiological datasets which may be easily compared between studies and laboratories. The outcome of an optimisation process undertaken in laboratories in India and the UK is described here, identifying four steps. First, samples were collected into a 2% (w/v) potassium dichromate solution. Second, oocysts were enriched by flotation in saturated saline. Third, genomic DNA was extracted using a QIAamp DNA Stool mini kit protocol including a mechanical homogenisation step. Finally, nested PCR was carried out using previously published primers targeting the internal transcribed spacer region 1 (ITS-1). Alternative methods tested included sample processing in the presence of faecal material, DNA extraction using a traditional phenol/chloroform protocol, the use of SCAR multiplex PCR (one tube and two tube versions) and speciation using the morphometric tool COCCIMORPH for the first time with field samples. PMID:24138724

EPA and DHA in blood cell membranes from acute coronary syndrome patients and controls.

Science.gov (United States)

Block, Robert C; Harris, William S; Reid, Kimberly J; Sands, Scott A; Spertus, John A

2008-04-01

Increased blood levels of the omega-3 fatty acids (FA) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been inversely associated with risk for sudden cardiac death, but their relationship with acute coronary syndromes (ACS) is unclear. We hypothesized that the EPA+DHA content of blood cell membranes, as a percent of total FAs, is reduced in ACS patients relative to matched controls. We measured the content of EPA+DHA in 768 ACS patients and 768 age-, sex- and race-matched controls. The association with ACS case status of blood cell EPA+DHA [both by a 1 unit change and by category (low, or =8%)] was assessed using multivariate conditional logistic regression models adjusting for matching variables and smoking status, alcohol use, diabetes, body mass index, serum lipids, education, family history of coronary artery disease, personal histories of myocardial infarction and hypertension, and statin, aspirin, and other antiplatelet drug use. The combined groups had a mean age of 61+/-12 years, 66% were male, and 92% were Caucasian. The EPA+DHA content was 20% lower in cases than controls (3.4+/-1.6 vs. 4.25+/-2.0%, pACS event was 0.58 (95% CI 0.42-0.80), in the intermediate EPA+DHA group and was 0.31 (95% CI 0.14-0.67; p for trend ACS case status increased incrementally as the EPA+DHA content decreased suggesting that low EPA+DHA may be associated with increased risk for ACS.

Engineering of EPA/DHA omega-3 fatty acid production by Lactococcus lactis subsp. cremoris MG1363.

Science.gov (United States)

Amiri-Jami, Mitra; Lapointe, Gisele; Griffiths, Mansel W

2014-04-01

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been shown to be of major importance in human health. Therefore, these essential polyunsaturated fatty acids have received considerable attention in both human and farm animal nutrition. Currently, fish and fish oils are the main dietary sources of EPA/DHA. To generate sustainable novel sources for EPA and DHA, the 35-kb EPA/DHA synthesis gene cluster was isolated from a marine bacterium, Shewanella baltica MAC1. To streamline the introduction of the genes into food-grade microorganisms such as lactic acid bacteria, unnecessary genes located upstream and downstream of the EPA/DHA gene cluster were deleted. Recombinant Escherichia coli harboring the 20-kb gene cluster produced 3.5- to 6.1-fold more EPA than those carrying the 35-kb DNA fragment coding for EPA/DHA synthesis. The 20-kb EPA/DHA gene cluster was cloned into a modified broad-host-range low copy number vector, pIL252m (4.7 kb, Ery) and expressed in Lactococcus lactis subsp. cremoris MG1363. Recombinant L. lactis produced DHA (1.35 ± 0.5 mg g(-1) cell dry weight) and EPA (0.12 ± 0.04 mg g(-1) cell dry weight). This is believed to be the first successful cloning and expression of EPA/DHA synthesis gene cluster in lactic acid bacteria. Our findings advance the future use of EPA/DHA-producing lactic acid bacteria in such applications as dairy starters, silage adjuncts, and animal feed supplements.

Level III Ecoregions of EPA Region 8

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions for EPA Administrative Regions were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the...

Springs, US EPA Region 9, 2013, SDWIS

Data.gov (United States)

U.S. Environmental Protection Agency — EPAâ??s Safe Drinking Water Information System (SDWIS) databases store information about drinking water. The federal version (SDWIS/FED) stores the information EPA...

Reservoirs, US EPA Region 9, 2013, SDWIS

Data.gov (United States)

U.S. Environmental Protection Agency — EPAâ??s Safe Drinking Water Information System (SDWIS) databases store information about drinking water. The federal version (SDWIS/FED) stores the information EPA...

EPA Facility Registry Service (FRS): ER_TRI

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): ER_TSCA

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): RCRA_LQG

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): AIRS_AFS

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): RCRA_TRANS

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

Level IV Ecoregions of EPA Region 8

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions for EPA Administrative Regions were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the...

Level IV Ecoregions of EPA Region 9

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions for EPA Administrative Regions were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the...

Level III Ecoregions of EPA Region 9

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions for EPA Administrative Regions were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the...

EPA Facility Registry Service (FRS): ER_RMP

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): ER_RCRATSD

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): ER_EPLAN

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): PCS_NPDES

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): ER_CERCLIS

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): CERCLIS_NPL

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that are...

EPA Facility Registry Service (FRS): ER_FRP

Data.gov (United States)

U.S. Environmental Protection Agency — This dataset contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link to Facility...

EPA Facility Registry Service (FRS): AIRS_AQS

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

USEPA Geospatial Metadata EPA Region 2 All Regulated Facilities GIS layer PUB.

Data.gov (United States)

U.S. Environmental Protection Agency — This ArcGIS 10.2 point feature class (All Regulated Facilities [R2] Public contains a unique record for every EPA Regulated Facility in EPA Region 2 (NYS, NJ, Puerto...

2004 Toxic Release Inventory Sites in Louisiana, Geographic NAD83, EPA (2006) [toxic_release_inventory_site_LA_EPA_2004

Data.gov (United States)

Louisiana Geographic Information Center — Data extracted from the EPA Toxics Release Inventory (TRI) system for reporting year 2004. The dataset contains facility identification, submitted and/or preferred...

Ad-Hoc vs. Standardized and Optimized Arthropod Diversity Sampling

Directory of Open Access Journals (Sweden)

Pedro Cardoso

2009-09-01

Full Text Available The use of standardized and optimized protocols has been recently advocated for different arthropod taxa instead of ad-hoc sampling or sampling with protocols defined on a case-by-case basis. We present a comparison of both sampling approaches applied for spiders in a natural area of Portugal. Tests were made to their efficiency, over-collection of common species, singletons proportions, species abundance distributions, average specimen size, average taxonomic distinctness and behavior of richness estimators. The standardized protocol revealed three main advantages: (1 higher efficiency; (2 more reliable estimations of true richness; and (3 meaningful comparisons between undersampled areas.

Endangered Species Case – Northwest Center for Alternatives to Pesticides v. EPA

Science.gov (United States)

EPA is reinstating streamside no-spray buffer zones to protect endangered or threatened Pacific salmon and steelhead in California, Oregon and Washington State, which were originally established in prior litigation brought against EPA by WTC and others.

Level IV Ecoregions of EPA Region 5

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level IV Ecoregions of EPA Region 10

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level IV Ecoregions of EPA Region 2

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level IV Ecoregions of EPA Region 3

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level III Ecoregions of EPA Region 2

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level III Ecoregions of EPA Region 4

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level IV Ecoregions of EPA Region 7

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level IV Ecoregions of EPA Region 6

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level IV Ecoregions of EPA Region 1

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level III Ecoregions of EPA Region 1

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level III Ecoregions of EPA Region 10

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level IV Ecoregions of EPA Region 4

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level III Ecoregions of EPA Region 3

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level III Ecoregions of EPA Region 7

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

EPA Facility Registry Service (FRS): Power Plants

Data.gov (United States)

U.S. Environmental Protection Agency — This GIS dataset contains data on power plants, based on the Energy Information Administration's EIA-860 dataset and supplemented with data from EPA's Facility...

Level III Ecoregions of EPA Region 5

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Level III Ecoregions of EPA Region 6

Data.gov (United States)

U.S. Environmental Protection Agency — Ecoregions by EPA region were extracted from the seamless national shapefile. Ecoregions denote areas of general similarity in ecosystems and in the type, quality,...

Enhanced Performance Assessment System (EPAS) for carbon sequestration.

Energy Technology Data Exchange (ETDEWEB)

Wang, Yifeng; Sun, Amy Cha-Tien; McNeish, Jerry A. (Sandia National Laboratories, Livermore, CA); Dewers, Thomas A.; Hadgu, Teklu; Jove-Colon, Carlos F.

2010-09-01

Carbon capture and sequestration (CCS) is an option to mitigate impacts of atmospheric carbon emission. Numerous factors are important in determining the overall effectiveness of long-term geologic storage of carbon, including leakage rates, volume of storage available, and system costs. Recent efforts have been made to apply an existing probabilistic performance assessment (PA) methodology developed for deep nuclear waste geologic repositories to evaluate the effectiveness of subsurface carbon storage (Viswanathan et al., 2008; Stauffer et al., 2009). However, to address the most pressing management, regulatory, and scientific concerns with subsurface carbon storage (CS), the existing PA methodology and tools must be enhanced and upgraded. For example, in the evaluation of a nuclear waste repository, a PA model is essentially a forward model that samples input parameters and runs multiple realizations to estimate future consequences and determine important parameters driving the system performance. In the CS evaluation, however, a PA model must be able to run both forward and inverse calculations to support optimization of CO{sub 2} injection and real-time site monitoring as an integral part of the system design and operation. The monitoring data must be continually fused into the PA model through model inversion and parameter estimation. Model calculations will in turn guide the design of optimal monitoring and carbon-injection strategies (e.g., in terms of monitoring techniques, locations, and time intervals). Under the support of Laboratory-Directed Research & Development (LDRD), a late-start LDRD project was initiated in June of Fiscal Year 2010 to explore the concept of an enhanced performance assessment system (EPAS) for carbon sequestration and storage. In spite of the tight time constraints, significant progress has been made on the project: (1) Following the general PA methodology, a preliminary Feature, Event, and Process (FEP) analysis was performed for

Quality Management Plan for EPA Region 1

Science.gov (United States)

The QMP describes policies, procedures & management systems within EPA NE that govern quality assurance & quality control activities supporting the transparency & scientific defensibility of environmental data collected, used & disseminated by the Region.

EPA Linked Open Data: Toxics Release Inventory

Data.gov (United States)

U.S. Environmental Protection Agency — TRI is a publicly available EPA database reported annually by certain covered industry groups, as well as federal facilities. It contains information about more than...

Methane Tracking and Mitigation Options - EPA CMOP

Data.gov (United States)

U.S. Environmental Protection Agency — This dataset contains the sub-model for EPA's MARKAL model, which tracks methane emissions from the energy system, and limited other sources (landfills and manure...

EPA Linked Open Data: Chemical Data Reporting

Data.gov (United States)

U.S. Environmental Protection Agency — This resource consists of the Chemical Data Reporting database that supports the Toxic Substances Control Act (TSCA) of 1976, which provides EPA with authority to...

US EPA - A*Star Partnership - Accelerating the Acceptance of ...

Science.gov (United States)

The path for incorporating new alternative methods and technologies into quantitative chemical risk assessment poses a diverse set of scientific challenges. Some of these challenges include development of relevant and predictive test systems and computational models to integrate and extrapolate experimental data, and rapid characterization and acceptance of these systems and models. The series of presentations will highlight a collaborative effort between the U.S. Environmental Protection Agency (EPA) and the Agency for Science, Technology and Research (A*STAR) that is focused on developing and applying experimental and computational models for predicting chemical-induced liver and kidney toxicity, brain angiogenesis, and blood-brain-barrier formation. In addressing some of these challenges, the U.S. EPA and A*STAR collaboration will provide a glimpse of what chemical risk assessments could look like in the 21st century. Presentation on US EPA – A*STAR Partnership at international symposium on Accelerating the acceptance of next-generation sciences and their application to regulatory risk assessment in Singapore.

Environmental protection belongs to the public: A vision for citizen science at EPA

Science.gov (United States)

Parker, A.; Dosemagen, S.

2017-12-01

As a collaborative and open approach to science, citizen science has the potential make science more actionable, applicable, and usable, especially when designed with scientists, communities and decision-makers as partners. In response to recent interest in citizen science from the US Environmental Protection Agency, the National Advisory Council for Environmental Policy and Technology provided EPA with advice and recommendations on how to integrate citizen science into the core work of EPA. The Council's 28 members—representatives of academia; business and industry; nongovernmental organizations; and state, local and tribal governments—identifies citizen science as an invaluable opportunity for EPA to strengthen public support for EPA's mission and the best approach for the Agency to connect with the public on environmental protection. The report recommends that EPA embrace citizen science as a core tenet of environmental protection, invest in citizen science for communities, partners, and the Agency, enable the use of citizen science data at the Agency, integrate citizen science into the full range of work of EPA. This presentation will outline principles and strategy for integrating citizen science into science and policy at the national level, increasing the usability of citizen science data for decision-making and policy, and leveraging citizen science for environmental protection.

Antibacterial and antibiofilm activities of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) against periodontopathic bacteria.

Science.gov (United States)

Sun, Mengjun; Zhou, Zichao; Dong, Jiachen; Zhang, Jichun; Xia, Yiru; Shu, Rong

2016-10-01

Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are two major omega-3 polyunsaturated fatty acids (n-3 PUFAs) with antimicrobial properties. In this study, we evaluated the potential antibacterial and antibiofilm activities of DHA and EPA against two periodontal pathogens, Porphyromonas gingivalis (P. gingivalis) and Fusobacterium nucleatum (F. nucleatum). MTT assay showed that DHA and EPA still exhibited no cytotoxicity to human oral tissue cells when the concentration came to 100 μM and 200 μM, respectively. Against P. gingivalis, DHA and EPA showed the same minimum inhibitory concentration (MIC) of 12.5 μM, and a respective minimum bactericidal concentration (MBC) of 12.5 μM and 25 μM. However, the MIC and MBC values of DHA or EPA against F. nucleatum were both greater than 100 μM. For early-stage bacteria, DHA or EPA displayed complete inhibition on the planktonic growth and biofilm formation of P. gingivalis from the lowest concentration of 12.5 μM. And the planktonic growth of F. nucleatum was slightly but not completely inhibited by DHA or EPA even at the concentration of 100 μM, however, the biofilm formation of F. nucleatum at 24 h was significantly restrained by 100 μM EPA. For exponential-phase bacteria, 100 μM DHA or EPA completely killed P. gingivalis and significantly decreased the viable counts of F. nucleatum. Meanwhile, the morphology of P. gingivalis was apparently damaged, and the virulence factor gene expression of P. gingivalis and F. nucleatum was strongly downregulated. Besides, the viability and the thickness of mature P. gingivalis biofilm, together with the viability of mature F. nucleatum biofilm were both significantly decreased in the presence of 100 μM DHA or EPA. In conclusion, DHA and EPA possessed antibacterial activities against planktonic and biofilm forms of periodontal pathogens, which suggested that DHA and EPA might be potentially supplementary therapeutic agents for prevention

EPA H2O User Manual

Science.gov (United States)

EPA H2O is a software tool designed to support research being conducted in the Tampa Bay watershed to provide information, data, and approaches and guidance that communities can use to examine alternatives when making strategic decisions to support a prosperous and environmentall...

Substance Identification Information from EPA's Substance Registry

Data.gov (United States)

U.S. Environmental Protection Agency — The Substance Registry Services (SRS) is the authoritative resource for basic information about substances of interest to the U.S. EPA and its state and tribal...

Meet EPA Scientist Jody Shoemaker, Ph.D.

Science.gov (United States)

EPA research chemist Jody Shoemaker, Ph.D., works to support Agency efforts to protect drinking water. She helps develop methods for analyzing organic chemicals on the Drinking Water Contaminant Candidate List (CCL).

Report: EPA Does Not Effectively Control or Monitor Imports of Hazardous Waste

Science.gov (United States)

Report #15-P-0172, July 6, 2015. The EPA lacks explicit authority to block imported shipments of hazardous waste that lack prior EPA consent. This could lead to improper handling and disposal, resulting in unknown human and environmental exposure to toxic

Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

KAUST Repository

De Vitis, Stefania; Matarise, Giuseppina; Pardeo, Francesca; Catalano, Rossella; Malara, Natalia Maria; Trunzo, Valentina; Tallerico, Rossana; Gentile, Francesco T.; Candeloro, Patrizio; Coluccio, Maria Laura; Massaro, Alessandro S.; Viglietto, Giuseppe; Carbone, Ennio; Kutter, Jö rg Peter; Perozziello, Gerardo; Di Fabrizio, Enzo M.

2014-01-01

The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs. © 2014 Elsevier B.V. All rights reserved.

Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

KAUST Repository

De Vitis, Stefania

2014-07-01

The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user\\'s needs. © 2014 Elsevier B.V. All rights reserved.

About Region 3's Laboratory and Field Services at EPA's Environmental Science Center

Science.gov (United States)

Mission & contact information for EPA Region 3's Laboratory and Field Services located at EPA's Environmental Science Center: the Office of Analytical Services and Quality Assurance & Field Inspection Program

Publication trends of study protocols in rehabilitation.

Science.gov (United States)

Jesus, Tiago S; Colquhoun, Heather L

2017-09-04

Growing evidence points for the need to publish study protocols in the health field. To observe whether the growing interest in publishing study protocols in the broader health field has been translated into increased publications of rehabilitation study protocols. Observational study using publication data and its indexation in PubMed. Not applicable. Not applicable. PubMed was searched with appropriate combinations of Medical Subject Headings up to December 2014. The effective presence of study protocols was manually screened. Regression models analyzed the yearly growth of publications. Two-sample Z-tests analyzed whether the proportion of Systematic Reviews (SRs) and Randomized Controlled Trials (RCTs) among study protocols differed from that of the same designs for the broader rehabilitation research. Up to December 2014, 746 publications of rehabilitation study protocols were identified, with an exponential growth since 2005 (r2=0.981; p<0.001). RCT protocols were the most common among rehabilitation study protocols (83%), while RCTs were significantly more prevalent among study protocols than among the broader rehabilitation research (83% vs. 35.8%; p<0.001). For SRs, the picture was reversed: significantly less common among study protocols (2.8% vs. 9.3%; p<0.001). Funding was more often reported by rehabilitation study protocols than the broader rehabilitation research (90% vs. 53.1%; p<0.001). Rehabilitation journals published a significantly lower share of rehabilitation study protocols than they did for the broader rehabilitation research (1.8% vs.16.7%; p<0.001). Identifying the reasons for these discrepancies and reverting unwarranted disparities (e.g. low rate of publication for rehabilitation SR protocols) are likely new avenues for rehabilitation research and its publication. SRs, particularly those aggregating RCT results, are considered the best standard of evidence to guide rehabilitation clinical practice; however, that standard can be improved

Meet EPA Chemist Quincy Teng, Ph.D.

Science.gov (United States)

EPA research chemist Quincy Teng, Ph.D., focuses on the application of metabolomics—a relatively new, specialized field of biochemistry focused on studying small molecules known as metabolites—on environmental and life sciences.

Meet EPA Biologist Thomas Knudsen, Ph.D.

Science.gov (United States)

Dr. Tom Knudsen is a developmental systems biologist at EPA's Center for Computational Toxicology. His research focuses on the potential for chemicals to disrupt prenatal development—one of the most important life stages.

Antihypertensive effect of eicosapentaenoic acid (EPA) on spontaneously hypertensive rats (SHR)

International Nuclear Information System (INIS)

Lam, B.K.; Quilley, J.; Hirai, A.; Yoshida, S.; Tamura, Y.; Wong, P.Y.K.

1986-01-01

EPA ethyl ester (99.8% pure) was administered orally (300 mg/kg/d) to adult (25-wk-old) and young (11 1/2-wk-old) SHR rats for 2 weeks at which time systolic blood pressure (BP), platelet aggregation, glomerular leukotriene (LT)A 4 hydrolase activity, plasma renin activity (PRA), urinary levels of TxB 2 and 6-keto-PGF/sub 1α/ and aortic conversion of [ 14 C]-AA to [ 14 C]-6-keto-PGF/sub 1α/ were measured. EPA treatment decreased BP of adult SHR with established hypertension from 238.7 +/- 2 to 217 +/- 4 mmHg (M +/- SD, P 4 hydrolase activity was inhibited. PRA and urinary levels of TxB 2 and 6-keto-PGF/sub 1α/ were not changed. Similarly, there was no change in aortic conversion of [ 14 C]-AA to [ 14 C]-6-keto-PGF/sub 1α/ indicating that EPA treatment does not alter vascular cyclo-oxygenase activity. These results indicate that EPA treatment affects eicosanoid metabolism and cardiovascular function

EPA H2O Software Tool

Science.gov (United States)

EPA H2O allows user to: Understand the significance of EGS in Tampa Bay watershed; visually analyze spatial distribution of the EGS in Tampa Bay watershed; obtain map and summary statistics of EGS values in Tampa Bay watershed; analyze and compare potential impacts of development...

EPA Region 1 No Discharge Zones

Science.gov (United States)

This dataset details No Discharge Zones (NDZ) for New England. Boaters may not discharge waste into these areas. Boundaries were determined mostly by Federal Register Environmental Documents in coordination with Massachusetts Coastal Zone Management (MA CZM) and EPA Region 1 Office of Ecosystem Protection (OEP) staff.

Meet EPA Scientist Jeff Szabo, Ph.D.

Science.gov (United States)

EPA scientist Jeff Szabo, Ph.D., has worked for the EPA’s National Homeland Security Research Center since 2005. He conducts and manages water security research projects at EPA’s Test and Evaluation facility.

Meet EPA Scientist Jordan West, Ph.D.

Science.gov (United States)

Jordan West, Ph.D. is an aquatic ecologist at EPA. Her areas of expertise include freshwater & marine ecology, climate change impacts and adaptation, resilience and threshold theory, environmental risk assessment, expert elicitation & stakeholder processes

Protocol for Cohesionless Sample Preparation for Physical Experimentation

Science.gov (United States)

2016-05-01

Standard test method for consolidated drained triaxial compression test for soils . In Annual book of ASTM standards. West Conshohocken, PA: ASTM...derived wherein uncertainties and laboratory scatter associated with soil fabric-behavior variance during sample preparation are mitigated. Samples of...wherein comparable analysis between different laboratory tests’ results can be made by ensuring a comparable soil fabric prior to laboratory testing

75 FR 14593 - Environmental Impact Statements and Regulations; Availability of EPA Comments

Science.gov (United States)

2010-03-26

... Management Plan for Reef Fish Resources, Addresses Bycatch of Sea Turtles in the Bottom Longline Component of... ENVIRONMENTAL PROTECTION AGENCY [ER-FRL-8989-4] Environmental Impact Statements and Regulations; Availability of EPA Comments Availability of EPA comments prepared pursuant to the Environmental Review Process...

VT US EPA Regulated Facilities Point Locations

Data.gov (United States)

Vermont Center for Geographic Information — (Link to Metadata) The EnvironPollution_ENVPTS2001 data layer is based on the U.S. EPA's Envirofacts point shapefile. The data was provided to VCGI by the Vermont...

Technical comments on EPA`s proposed revisions to the National Ambient Air Quality Standard for particulate matter

Energy Technology Data Exchange (ETDEWEB)

Lipfert, F.W.

1997-03-01

The US Environmental Protection Agency (EPA) has proposed new ambient air quality standards specifically for fine particulate matter, regulating concentrations of particles with median aerodynamic diameters less than 2.5 {mu}m (PM{sub 2.5}). Two new standards have been proposed: a maximum 24-hr concentration that is intended to protect against acute health effects, and an annual concentration limit that is intended to protect against longer-term health effects. EPA has also proposed a slight relaxation of the 24-hr standard for inhalable particles (PM{sub 10}), by allowing additional exceedances each year. Fine particles are currently being indirectly controlled by means of regulations for PM{sub 10} and TSP, under the Clean Air Act of 1970 and subsequent amendments. Although routine monitoring of PM{sub 2.5} is rare and data are sparse, the available data indicate that ambient concentrations have been declining at about 6% per year under existing regulations.

External quality control in ground-water sampling and analysis at the Hanford Site

International Nuclear Information System (INIS)

Hall, S.H.; Juracich, S.P.

1991-11-01

At the US Department of Energy's Hanford Site, external Quality Control (QC) for ground-water monitoring is extensive and has included routine submittal of intra- and interlaboratory duplicate samples, blind samples, and several kinds of blank samples. Examination of the resulting QC data for nine of the constituents found in ground water at the Hanford Site shows that the quality of analysis has generally been within the expectations of precision and accuracy that have been established by the US Environmental Protection Agency (EPA). The constituents subjected to review were nitrate, chromium, sodium, fluoride, carbon tetrachloride, tritium, ammonium, trichloroethylene, and cyanide. Of these, the fluoride measurements were notable exceptions and were poor by EPA standards. The review has shown that interlaboratory analysis of duplicate samples yields the most useful QC data for evaluating laboratory performance in determining commonly encountered constituents. For rarely encountered constituents, interlaboratory comparisons may be augmented with blind samples (synthetic samples of known composition). Intralaboratory comparisons, blanks, and spikes should be generally restricted to studies of suspected or known sample contamination and to studies of the adequacy of sampling and analytical procedures

Risk Management Plan (RMP) Facility Points, Region 9, 2011, US EPA Region 9

Data.gov (United States)

U.S. Environmental Protection Agency — Risk Management Plan (RMP): Under the Clean Air Act, Section 112(r), the EPA established a program requiring risk management plans to be provided to the EPA by...

Risk Management Plan (RMP) Facility Points, Region 9, 2014, US EPA Region 9

Data.gov (United States)

U.S. Environmental Protection Agency — Risk Management Plan (RMP): Under the Clean Air Act, Section 112(r), the EPA established a program requiring risk management plans to be provided to the EPA by...

Checklist for Reviewing EPA Quality Management Plans

Science.gov (United States)

This checklist will be used to review the Quality Management Plans (QMPs) that are submitted to the Quality Staff of the Office of Environmental Information (OEI) for Agency review under EPA Order 5360.1 A2.

40 CFR 1068.15 - What general provisions apply for EPA decision-making?

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 32 2010-07-01 2010-07-01 false What general provisions apply for EPA decision-making? 1068.15 Section 1068.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Miscellaneous Provisions § 1068.15 What general provisions apply for EPA decision-making? (a) The Administrator...

Identification of a research protocol to study orthodontic tooth movement

Directory of Open Access Journals (Sweden)

Annalisa Dichicco

2014-06-01

Full Text Available Aim: The orthodontic movement is associated with a process of tissue remodeling together with the release of several chemical mediators in periodontal tissues. Each mediator is a potential marker of tooth movement and expresses biological processes as: tissue inflammation and bone remodeling. Different amounts of every mediator are present in several tissues and fluids of the oral cavity. Therefore, there are different methods that allow sampling with several degrees of invasiveness. Chemical mediators are also substances of different molecular nature, and multiple kind of analysis methods allow detection. The purpose of this study was to draft the best research protocol for an optimal study on orthodontic movement efficiency. Methods: An analysis of the international literature have been made, to identify the gold standard of each aspect of the protocol: type of mediator, source and method of sampling and analysis method. Results: From the analysis of the international literature was created an original research protocol for the study and the assessment of the orthodontic movement, by using the biomarkers of the tooth movement. Conclusions: The protocol created is based on the choice of the gold standard of every aspect already analyzed in the literature and in existing protocols for the monitoring of orthodontic tooth movement through the markers of tooth movement. Clinical trials re required for the evaluation and validation of the protocol created.

Meet EPA Chemist Mark Strynar, Ph.D.

Science.gov (United States)

Dr. Mark Strynar is a physical scientist in EPA's Office of Research and Development. His research interests include developing methods to measure and analyze the movement of PFCs and other xenobiotic compounds in biological and environmental media

Meet EPA Scientist Susan Yee, Ph.D.

Science.gov (United States)

Susan Yee, Ph.D., is an ecologist at EPA's Gulf Ecology Division. She is working on the Puerto Rico Sustainable Communities program, developing decision support tools to evaluate how alternative decisions impact coastal ecosystem goods and services

Au nanoparticles grafted on Fe{sub 3}O{sub 4} as effective SERS substrates for label-free detection of the 16 EPA priority polycyclic aromatic hydrocarbons

Energy Technology Data Exchange (ETDEWEB)

Du, Jingjing; Xu, Jianwei; Sun, Zhenli; Jing, Chuanyong, E-mail: cyjing@rcees.ac.cn

2016-04-07

Several methods and materials have been explored for the sensitive and practicable detection of polycyclic aromatic hydrocarbons (PAHs). However, it is still a challenge to develop simple and cost-effective sensing techniques for PAHs. Herein we report the synthesis and construction of Fe{sub 3}O{sub 4}@Au SERS substrate. This magnetic substrate was composed by Fe{sub 3}O{sub 4} microspheres and Au NPs. The size, morphology, and surface composition of Fe{sub 3}O{sub 4}@Au were characterized by multiple complimentary techniques including scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray powder diffraction. The spatial distributions of electro-magnetic field enhancement around Fe{sub 3}O{sub 4}@Au was calculated using finite difference time domain (FDTD) simulations. As a result of its remarkable sensitivity, the Fe{sub 3}O{sub 4}@Au-based SERS assay has been applied to detect the 16 EPA priority PAHs. The LODs achieved by our method (100–5 nM, 16.6–1.01 μg L{sup −1}) make it promising for the rapid screening of highly contaminated cases. As a proof-of-concept study, the substrate was applied in SERS sensing of PAHs in river matrix. The 16 PAHs could be differentiated based upon their characteristic SERS peaks. Most importantly, the detection was successfully conducted using a portable Raman spectrometer, which could be used for on-site monitoring of PAHs. - Highlights: • SERS detection of the 16 EPA priority PAHs was conducted. • The satellite-core structure lead to high SERS enhancement by confined hotspots. • The approach does not require expensive instrumentation or large sample volumes. • The effective protocol is useful for the identification of hydrophobic molecules.

EPA and the Federal Technology Transfer Act: Opportunity knocks

Energy Technology Data Exchange (ETDEWEB)

Gatchett, A.M.; Fradkin, L.; Moore, M.; Gorman, T.; Ehrlich, A. [Environmental Protection Agency, Washington, DC (United States)

1990-12-31

In 1986, the Federal Technology Transfer Act (FTTA) was established to promote a closer, collaborative relationship between federal government agencies and the private sector. With the increasing need for new cost-effective technologies to prevent and control pollution, both the US Environmental Protection Agency (EPA) and private industry are encouraged to facilitate the transfer of knowledge and technology under this Act. The FTTA removed several of the legal and institutional barriers to cooperative research that existed before the Act`s passage. Through the FTTA, the government strives to promote the movement of its products, processes, skills, and knowledge into the private sector for further development and commercialization by encouraging the exchange of technical personnel and the sharing of facilities and other resources. Collaborative efforts between industry, federal agencies, and academia are made possible through cooperative research and development agreements (CRADAs). Forty-two CRADAs and five licensing agreements have been initiated with EPA under this program. This paper provides an overview of this new and innovative program within the EPA. 1 fig., 2 tabs.

EPA's Review of DOE's Inventory Tracking for TRU Wastes at Waste Control Specialists

Science.gov (United States)

On April 9, 2014, EPA's Waste Isolation Pilot Plant (WIPP) waste characterization team visited Waste Control Specialists (WCS) to determine whether DOE was meeting EPA's waste inventory tracking requirements at 40 CFR 194.24(c)(4).

Plan for implementing EPA standards for UMTRA sites

International Nuclear Information System (INIS)

1984-01-01

This plan is designed to be a generic presentation on methodology that will be followed in implementing the EPA standards. Its applicability covers 24 inactive uranium mill tailings sites and approximately 8000 vicinity properties, no two of which are alike. This diversity dictates the more general approach of this plan. In practice, however, site-specific application will be implemented and will require extensive consultation with the appropriate state or tribe and the NRC. In addition, information concerning relevant Federal, state, or tribal standards and regulations will be considered along with any data that may assist in the evaluations. Throughout this process, DOE will encourage state and tribal participation to ensure that compliance with the EPA standards will be achieved

US EPA's experiences implementing environmental safety standards at the Waste Isolation Pilot Plant - 16103

International Nuclear Information System (INIS)

Peake, R. Thomas; Byrum, Charles; Feltcorn, Ed; Lee, Raymond; Joglekar, Rajani; Ghose, Shankar; Eagle, Mike

2009-01-01

The U.S. Environmental Protection Agency (EPA or the Agency) developed environmental standards for the disposal of defense-related transuranic wastes for the U.S. Department of Energy's (DOE or the Department) Waste Isolation Pilot Plant (WIPP). EPA implements these standards for WIPP, which has been in operation for over ten years. The general environmental standards are set forth in the Agency's 40 CFR Part 191 Environmental Radiation Protection Standards for the Management and Disposal of Spent Nuclear Fuel, High-Level and Transuranic Radioactive Wastes [1]. These standards are implemented by site-specific compliance criteria [2]. The WIPP Land Withdrawal Act requires DOE to submit a re-certification application every five years after the initial receipt of waste. DOE submitted the latest WIPP re-certification application in March 2009. For re-certification, DOE must identify changes that have occurred over the previous five years and analyze their impact on the potential long-term performance of the repository. Once EPA determines that the re-certification application is complete, the Agency has six months to review the application and make a final decision. During this review, EPA solicits and incorporates public comment where appropriate. During the first re-certification in 2004, several stakeholder groups brought up issues (e.g., karst) that were addressed in the original certification. EPA has received comments again raising some of these same issues for the 2009 re-certification. In addition, DOE must submit proposed changes to the WIPP repository to EPA for review and approval. This paper describes selected issues of concern to WIPP and highlights interactions between EPA as the regulatory authority and DOE as the implementing organization. In general EPA's experience points out the importance of communication, documentation and the regulator's responsibility in determining 'how much is enough'. (authors)

Detection and identification of Leishmania spp.: application of two hsp70-based PCR-RFLP protocols to clinical samples from the New World.

Science.gov (United States)

Montalvo, Ana M; Fraga, Jorge; Tirado, Dídier; Blandón, Gustavo; Alba, Annia; Van der Auwera, Gert; Vélez, Iván Darío; Muskus, Carlos

2017-07-01

Leishmaniasis is highly prevalent in New World countries, where several methods are available for detection and identification of Leishmania spp. Two hsp70-based PCR protocols (PCR-N and PCR-F) and their corresponding restriction fragment length polymorphisms (RFLP) were applied for detection and identification of Leishmania spp. in clinical samples recruited in Colombia, Guatemala, and Honduras. A total of 93 cases were studied. The samples were classified into positive or suspected of leishmaniasis according to parasitological criteria. Molecular amplification of two different hsp70 gene fragments and further RFLP analysis for identification of Leishmania species was done. The detection in parasitologically positive samples was higher using PCR-N than PCR-F. In the total of samples studied, the main species identified were Leishmania panamensis, Leishmania braziliensis, and Leishmania infantum (chagasi). Although RFLP-N was more efficient for the identification, RFLP-F is necessary for discrimination between L. panamensis and Leishmania guyanesis, of great importance in Colombia. Unexpectedly, one sample from this country revealed an RFLP pattern corresponding to Leishmania naiffi. Both molecular variants are applicable for the study of clinical samples originated in Colombia, Honduras, and Guatemala. Choosing the better tool for each setting depends on the species circulating. More studies are needed to confirm the presence of L. naiffi in Colombian territory.

Computational Toxicology as Implemented by the US EPA ...

Science.gov (United States)

Computational toxicology is the application of mathematical and computer models to help assess chemical hazards and risks to human health and the environment. Supported by advances in informatics, high-throughput screening (HTS) technologies, and systems biology, the U.S. Environmental Protection Agency EPA is developing robust and flexible computational tools that can be applied to the thousands of chemicals in commerce, and contaminant mixtures found in air, water, and hazardous-waste sites. The Office of Research and Development (ORD) Computational Toxicology Research Program (CTRP) is composed of three main elements. The largest component is the National Center for Computational Toxicology (NCCT), which was established in 2005 to coordinate research on chemical screening and prioritization, informatics, and systems modeling. The second element consists of related activities in the National Health and Environmental Effects Research Laboratory (NHEERL) and the National Exposure Research Laboratory (NERL). The third and final component consists of academic centers working on various aspects of computational toxicology and funded by the U.S. EPA Science to Achieve Results (STAR) program. Together these elements form the key components in the implementation of both the initial strategy, A Framework for a Computational Toxicology Research Program (U.S. EPA, 2003), and the newly released The U.S. Environmental Protection Agency's Strategic Plan for Evaluating the T

Evolution of EPA/DOE technical cooperation in remediation of radiation/mixed waste contaminated sites

International Nuclear Information System (INIS)

Dyer, Robert S.; Garcia-Frias, Beverly; Wolbarst, Anthony B.; Coe, Larry J.

1992-01-01

The EPA Office of Radiation Programs (ORP) and the DOE Office of Environmental Restoration and Waste Management (EM) are cooperating in efforts related to restoration of radioactive and mixed waste sites. The impetus for these efforts derived from DOE's need to perform restoration activities according to CERCLA/RCRA requirements, and from ORP's role as a supplier of radiation expertise to federal agencies. These activities include: assessing remediation technology, developing radioanalytical protocols; matching cleanup technologies to soil characteristics; developing a process for the evaluation, selection, and appropriate use of groundwater models; reviewing incinerator practices; and addressing technical issues associated with the WIPP. Cooperative projects planned for the future include: evaluation of methodologies for streamlining the restoration process; assessment of the applicability of process knowledge for waste characterization; evaluation of recycling of radioactive metals; and expansion of selected environmental protection initiatives at the International Atomic Energy Agency (IAEA). Public acceptance is a crucial component of the remediation process. An underlying objective of these cooperative initiatives is to address issues of concern to the public in an open and honest fashion. (author)

EPA Monthly Key Performance Indicator Dashboards 2018

Science.gov (United States)

2018 reports are added each month, which measure how well EPA web content is meeting three performance goals: increases in how much users consume content, are able to find or discover what they need, and their level of engagement.

78 FR 40138 - Notification of Deletion of System of Records: Kid's Club Membership List (EPA-57)

Science.gov (United States)

2013-07-03

... children. Participants received certificates, membership cards and stickers for joining the club. Completed... System of Records: Kid's Club Membership List (EPA-57) AGENCY: Environmental Protection Agency (EPA... Kids Club Membership List (EPA-57) published in the Federal Register on March 2, 2006, from its...

summarytable.PNG | ECHO | US EPA

Science.gov (United States)

ECHO, Enforcement and Compliance History Online, provides compliance and enforcement information for approximately 800,000 EPA-regulated facilities nationwide. ECHO includes permit, inspection, violation, enforcement action, and penalty information about facilities regulated under the Clean Air Act (CAA) Stationary Source Program, Clean Water Act (CWA) National Pollutant Elimination Discharge System (NPDES), and/or Resource Conservation and Recovery Act (RCRA). Information also is provided on surrounding demographics when available.

Resources.PNG | ECHO | US EPA

Science.gov (United States)

ECHO, Enforcement and Compliance History Online, provides compliance and enforcement information for approximately 800,000 EPA-regulated facilities nationwide. ECHO includes permit, inspection, violation, enforcement action, and penalty information about facilities regulated under the Clean Air Act (CAA) Stationary Source Program, Clean Water Act (CWA) National Pollutant Elimination Discharge System (NPDES), and/or Resource Conservation and Recovery Act (RCRA). Information also is provided on surrounding demographics when available.

Custom Search | ECHO | US EPA

Science.gov (United States)

ECHO, Enforcement and Compliance History Online, provides compliance and enforcement information for approximately 800,000 EPA-regulated facilities nationwide. ECHO includes permit, inspection, violation, enforcement action, and penalty information about facilities regulated under the Clean Air Act (CAA) Stationary Source Program, Clean Water Act (CWA) National Pollutant Elimination Discharge System (NPDES), and/or Resource Conservation and Recovery Act (RCRA). Information also is provided on surrounding demographics when available.

Watershed Statistics | ECHO | US EPA

Science.gov (United States)

ECHO, Enforcement and Compliance History Online, provides compliance and enforcement information for approximately 800,000 EPA-regulated facilities nationwide. ECHO includes permit, inspection, violation, enforcement action, and penalty information about facilities regulated under the Clean Air Act (CAA) Stationary Source Program, Clean Water Act (CWA) National Pollutant Elimination Discharge System (NPDES), and/or Resource Conservation and Recovery Act (RCRA). Information also is provided on surrounding demographics when available.

May 2011 Groundwater and Surface Water Sampling at the Rio Blanco, Colorado, Site (Data Validation Package)

International Nuclear Information System (INIS)

2011-01-01

Annual sampling was conducted at the Rio Blanco, Colorado, site for the Long-Term Hydrologic Monitoring Program May 16-17, 2011, to monitor groundwater and surface water for potential radionuclide contamination. Sampling and analyses were conducted as specified in Sampling and Analysis Plan for the U.S. Department of Energy Office of Legacy Management Sites (LMS/PRO/S04351, continually updated). A duplicate sample was collected from location Johnson Artesian WL. Samples were analyzed by the U.S. Environmental Protection Agency (EPA) Radiation&Indoor Environments National Laboratory in Las Vegas, Nevada. Samples were analyzed for gamma-emitting radionuclides by high-resolution gamma spectrometry, and for tritium using the conventional method. Tritium was not measured using the enrichment method because the EPA laboratory no longer offers that service. Results of this monitoring at the Rio Blanco site demonstrate that groundwater and surface water outside the boundaries have not been affected by project-related contaminants.

Distribution Systems, US EPA Region 9, 2013, SDWIS

Data.gov (United States)

U.S. Environmental Protection Agency — EPAâ??s Safe Drinking Water Information System (SDWIS) databases store information about drinking water. The federal version (SDWIS/FED) stores the information EPA...

Water Storage, US EPA Region 9, 2013, SDWIS

Data.gov (United States)

U.S. Environmental Protection Agency — EPAâ??s Safe Drinking Water Information System (SDWIS) databases store information about drinking water. The federal version (SDWIS/FED) stores the information EPA...

Pumping Facilities, US EPA Region 9, 2013, SDWIS

Data.gov (United States)

U.S. Environmental Protection Agency — EPAâ??s Safe Drinking Water Information System (SDWIS) databases store information about drinking water. The federal version (SDWIS/FED) stores the information EPA...

Treatment Plants, US EPA Region 9, 2013, SDWIS

Data.gov (United States)

U.S. Environmental Protection Agency — EPAâ??s Safe Drinking Water Information System (SDWIS) databases store information about drinking water. The federal version (SDWIS/FED) stores the information EPA...

EPA Facility Registry Service (FRS): PCS_NPDES_MAJOR

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that are...

EPA Facility Registry Service (FRS): ER_WWTP_NPDES

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of Waste Water Treatment...

EPA Toxicologists Focus Innovative Research on PFAS Compounds

Science.gov (United States)

EPA researchers have partnered with researchers at the National Toxicology Program to develop a tiered testing approach to quickly generate toxicity and kinetic information for approximately 75 PFAS compounds.

EPA's Role in International Environment, Trade and Finance

Science.gov (United States)

Both domestically and globally, protecting human health and the environment is essential to sustainable economic growth and development. EPA works in trade, environment and finance to protect these goals.

EPA Facility Registry Service (FRS): AIRS_AFS_MAJOR

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

76 FR 9988 - Improving EPA Regulations

Science.gov (United States)

2011-02-23

... suggestions do you have for how the Agency could change the regulations to be more flexible? 6. Benefits and... public input on the design of a plan to use for periodic retrospective review of its regulations. DATES... At this time, EPA seeks help in designing the plan it will use for periodic review of regulations...

EPA Region 2 Draft NPL Site Contamination Area Boundaries as of February 2007 GIS Layer [EPA.R2_NPL_CONTAMBND

Data.gov (United States)

U.S. Environmental Protection Agency — This layer represents the contamination boundaries of all NPL sites located in EPA Region Region 2 (New York, New Jersey, Puerto Rico and the U.S. Virgin Islands)....

EPA Facility Registry Service (FRS): Facility Interests Dataset

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service consists of location and facility identification information from EPA's Facility Registry Service (FRS) for all sites that are available in...

40 CFR 59.409 - Addresses of EPA Offices.

Science.gov (United States)

2010-07-01

..., Tennessee), Director, Air, Pesticides, and Toxics Management Division, 61 Forsyth Street, Atlanta, GA 30303... Division, 77 West Jackson Boulevard, Chicago, IL 60604-3507. EPA Region VI (Arkansas, Louisiana, New Mexico...

Environmental Finance Center Serving EPA's Region 8 States

Science.gov (United States)

The National Rural Water Association, headquartered in Duncan Oklahoma, has been selected through a competitive grants process to establish a regional Environmental Finance Center (EFC) serving EPA Region 8 states.

75 FR 13275 - Environmental Impact Statements and Regulations; Availability of EPA Comments

Science.gov (United States)

2010-03-19

... (ERP), under section 309 of the Clean Air Act and Section 102(2)(copyright) of the National... notice of availability of EPA comments in the Federal Register. Draft EISs EIS No. 20090438, ERP No. D..., Hyde Park, NY. Summary: EPA does not object to the proposed action. Rating LO. EIS No. 20100016, ERP No...

An urgent need for an EPA standard for disposal of coal ash

International Nuclear Information System (INIS)

Lemly, A. Dennis

2014-01-01

EPA, the White House, and electric utilities are stalled in a struggle over a proposed new rule on coal ash disposal. Although this rule is long overdue, EPA now stands on the cusp of bringing forward a landmark decision that could benefit aquatic resources in the USA for decades to come and also set an important regulatory leadership example for the international community to follow. However, multi-million dollar wildlife losses are continuing to pile up as things stall in Washington. In this commentary I use a newly reported example, Wildlife Damage Case 23, to further illustrate serious flaws in the National Pollutant Discharge Elimination System that EPA's new rule can address. Case 23 provides additional impetus for EPA and the White House to move swiftly and decisively to end surface impoundment disposal of coal ash and the associated toxic impacts to wildlife. - Wildlife poisoning from coal combustion waste shows how regulatory policy is influenced by politics and industry rather than prudent decisions based on credible scientific investigation

On-farm comparisons of different cleaning protocols in broiler houses.

Science.gov (United States)

Luyckx, K Y; Van Weyenberg, S; Dewulf, J; Herman, L; Zoons, J; Vervaet, E; Heyndrickx, M; De Reu, K

2015-08-01

The present study evaluated the effectiveness of 4 cleaning protocols designed to reduce the bacteriological infection pressure on broiler farms and prevent food-borne zoonoses. Additionally, difficult to clean locations and possible sources of infection were identified. Cleaning and disinfection rounds were evaluated in 12 broiler houses on 5 farms through microbiological analyses and adenosine triphosphate hygiene monitoring. Samples were taken at 3 different times: before cleaning, after cleaning, and after disinfection. At each sampling time, swabs were taken from various locations for enumeration of the total aerobic flora and Enterococcus species pluralis ( SPP:). In addition, before cleaning and after disinfection, testing for Escherichia coli and Salmonella was carried out. Finally, adenosine triphosphate swabs and agar contact plates for total aerobic flora counts were taken after cleaning and disinfection, respectively. Total aerobic flora and Enterococcus spp. counts on the swab samples showed that cleaning protocols which were preceded by an overnight soaking with water caused a higher bacterial reduction compared to protocols without a preceding soaking step. Moreover, soaking of broiler houses leads to less water consumption and reduced working time during high pressure cleaning. No differences were found between protocols using cold or warm water during cleaning. Drinking cups, drain holes, and floor cracks were identified as critical locations for cleaning and disinfection in broiler houses. © 2015 Poultry Science Association Inc.

PREPAR: a user-friendly preprocessor to create AIRDOS-EPA input data sets

International Nuclear Information System (INIS)

Sjoreen, A.L.; Miller, C.W.; Nelson, C.B.

1984-01-01

PREPAR is a FORTRAN program designed to simplify the preparation of input for the AIRDOS-EPA computer code. PREPAR was designed to provide a method for data entry that is both logical and flexible. It also provides default values for all variables, so the user needs only to enter those data for which the defaults should be changed. Data are entered either unformatted or via a user-selected format. A separate file of the nuclide-specific data needed by AIRDOS-EPA is read by PREPAR. Two utility programs, EXTRAC and RADLST, were written to create and list this file. PREPAR writes the file needed to run AIRDOS-EPA and writes a listing of that file

The Protocol of Choice for Treatment of Snake Bite

Directory of Open Access Journals (Sweden)

Afshin Mohammad Alizadeh

2016-01-01

Full Text Available The aim of the current study is to compare three different methods of treatment of snake bite to determine the most efficient one. To unify the protocol of snake bite treatment in our center, we retrospectively reviewed files of the snake-bitten patients who had been referred to us between 2010 and 2014. They were contacted for follow-up using phone calls. Demographic and on-arrival characteristics, protocol used for treatment (WHO/Haddad/GF, and outcome/complications were evaluated. Patients were entered into one of the protocol groups and compared. Of a total of 63 patients, 56 (89% were males. Five, 19, and 28 patients were managed by Haddad, WHO, or GF protocols, respectively. Eleven patients had fallen into both GF and WHO protocols and were excluded. Serum sickness was significantly more common when WHO protocol was used while 100% of the compartment syndromes and 71% of deformities had been reported after GF protocol. The most important complications were considered to be deformity, compartment syndrome, and amputation and were more frequent after the use of WHO and GF protocols (23.1% versus 76.9%; none in Haddad; P = NS. Haddad protocol seems to be the best for treatment of snake-bitten patients in our region. However, this cannot be strictly concluded because of the limited sample size and nonsignificant P values.

Entrustable professional activity (EPA) reshapes the practice of specialist training.

Science.gov (United States)

Niemi-Murola, Leila

In addition to medical expertise, competence-based medical training comprises communication and collaboration skills, professionalism, and leadership skills. Continuous feedback is essential for learning and development, and feedback only from the medical specialist examination taken in the end of training does not ensure thorough specialist training. Entrustable professional activity (EPA) is a unit of professional practice, defined as tasks or responsibilities typical of the specialty. EPA translates competence-based training into manageable and meaningful entities and provides tools for the evaluation of medical competence.

EPA Region 9 Targeted Brownfields Assessment (TBA) Application

Science.gov (United States)

EPA provides contractor assistance for property assessment to determine eligibility for up to $100,000 worth of technical assistance. Use this assessment form for properties in Arizona, California, Nevada, and Hawaii.

(n-3) fatty acids and cardiovascular health: are effects of EPA and DHA shared or complementary?

Science.gov (United States)

Mozaffarian, Dariush; Wu, Jason H Y

2012-03-01

Considerable research supports cardiovascular benefits of consuming omega-3 PUFA, also known as (n-3) PUFA, from fish or fish oil. Whether individual long-chain (n-3) PUFA have shared or complementary effects is not well established. We reviewed evidence for dietary and endogenous sources and cardiovascular effects on biologic pathways, physiologic risk factors, and clinical endpoints of EPA [20:5(n-3)], docosapentaenoic acid [DPA, 22:5(n-3)], and DHA [22:6(n-3)]. DHA requires direct dietary consumption, with little synthesis from or retroconversion to DPA or EPA. Whereas EPA is also largely derived from direct consumption, EPA can also be synthesized in small amounts from plant (n-3) precursors, especially stearidonic acid. In contrast, DPA appears principally derived from endogenous elongation from EPA, and DPA can also undergo retroconversion back to EPA. In experimental and animal models, both EPA and DHA modulate several relevant biologic pathways, with evidence for some differential benefits. In humans, both fatty acids lower TG levels and, based on more limited studies, favorably affect cardiac diastolic filling, arterial compliance, and some metrics of inflammation and oxidative stress. All three (n-3) PUFA reduce ex vivo platelet aggregation and DHA also modestly increases LDL and HDL particle size; the clinical relevance of such findings is uncertain. Combined EPA+DHA or DPA+DHA levels are associated with lower risk of fatal cardiac events and DHA with lower risk of atrial fibrillation, suggesting direct or indirect benefits of DHA for cardiac arrhythmias (although not excluding similar benefits of EPA or DPA). Conversely, EPA and DPA, but not DHA, are associated with lower risk of nonfatal cardiovascular endpoints in some studies, and purified EPA reduced risk of nonfatal coronary syndromes in one large clinical trial. Overall, for many cardiovascular pathways and outcomes, identified studies of individual (n-3) PUFA were relatively limited, especially

Two-Stage Enzymatic Preparation of Eicosapentaenoic Acid (EPA) And Docosahexaenoic Acid (DHA) Enriched Fish Oil Triacylglycerols.

Science.gov (United States)

Zhang, Zhen; Liu, Fang; Ma, Xiang; Huang, Huihua; Wang, Yong

2018-01-10

Fish oil products in the form of triacylglycerols generally have relatively low contents of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and so it is of potential research and industrial interest to enrich the related contents in commercial products. Thereby an economical and efficient two-stage preparation of EPA and DHA enriched fish oil triacylglycerols is proposed in this study. The first stage was the partial hydrolysis of fish oil by only 0.2 wt.‰ AY "Amano" 400SD which led to increases of EPA and DHA contents in acylglycerols from 19.30 and 13.09 wt % to 25.95 and 22.06 wt %, respectively. Subsequently, products of the first stage were subjected to transesterification with EPA and DHA enriched fatty acid ethyl esters (EDEE) as the second stage to afford EPA and DHA enriched fish oil triacylglycerols by using as low as 2 wt % Novozyme 435. EDEEs prepared from fish oil ethyl ester, and recycled DHA and EPA, respectively, were applied in this stage. Final products prepared with two different sources of EDEEs were composed of 97.62 and 95.92 wt % of triacylglycerols, respectively, with EPA and DHA contents of 28.20 and 21.41 wt % for the former and 25.61 and 17.40 wt % for the latter. Results not only demonstrate this two-stage process's capability and industrial value for enriching EPA and DHA in fish oil products, but also offer new opportunities for the development of fortified fish oil products.

Protein blotting protocol for beginners.

Science.gov (United States)

Petrasovits, Lars A

2014-01-01

The transfer and immobilization of biological macromolecules onto solid nitrocellulose or nylon (polyvinylidene difluoride (PVDF)) membranes subsequently followed by specific detection is referred to as blotting. DNA blots are called Southerns after the inventor of the technique, Edwin Southern. By analogy, RNA blots are referred to as northerns and protein blots as westerns (Burnette, Anal Biochem 112:195-203, 1981). With few exceptions, western blotting involves five steps, namely, sample collection, preparation, separation, immobilization, and detection. In this chapter, protocols for the entire process from sample collection to detection are described.

Simulating quantum correlations as a distributed sampling problem

International Nuclear Information System (INIS)

Degorre, Julien; Laplante, Sophie; Roland, Jeremie

2005-01-01

It is known that quantum correlations exhibited by a maximally entangled qubit pair can be simulated with the help of shared randomness, supplemented with additional resources, such as communication, postselection or nonlocal boxes. For instance, in the case of projective measurements, it is possible to solve this problem with protocols using one bit of communication or making one use of a nonlocal box. We show that this problem reduces to a distributed sampling problem. We give a new method to obtain samples from a biased distribution, starting with shared random variables following a uniform distribution, and use it to build distributed sampling protocols. This approach allows us to derive, in a simpler and unified way, many existing protocols for projective measurements, and extend them to positive operator value measurements. Moreover, this approach naturally leads to a local hidden variable model for Werner states

75 FR 10261 - Request for Nominations to the EPA Human Studies Review Board

Science.gov (United States)

2010-03-05

... scientific and ethical aspects of human subjects research. The major objectives of the HSRB are to provide... of the following areas: Bioethics: expertise in the ethics of research with human subjects... EPA Human Studies Review Board AGENCY: Environmental Protection Agency (EPA). ACTION: Notice. SUMMARY...

An electronic specimen collection protocol schema (eSCPS). Document architecture for specimen management and the exchange of specimen collection protocols between biobanking information systems.

Science.gov (United States)

Eminaga, O; Semjonow, A; Oezguer, E; Herden, J; Akbarov, I; Tok, A; Engelmann, U; Wille, S

2014-01-01

The integrity of collection protocols in biobanking is essential for a high-quality sample preparation process. However, there is not currently a well-defined universal method for integrating collection protocols in the biobanking information system (BIMS). Therefore, an electronic schema of the collection protocol that is based on Extensible Markup Language (XML) is required to maintain the integrity and enable the exchange of collection protocols. The development and implementation of an electronic specimen collection protocol schema (eSCPS) was performed at two institutions (Muenster and Cologne) in three stages. First, we analyzed the infrastructure that was already established at both the biorepository and the hospital information systems of these institutions and determined the requirements for the sufficient preparation of specimens and documentation. Second, we designed an eSCPS according to these requirements. Finally, a prospective study was conducted to implement and evaluate the novel schema in the current BIMS. We designed an eSCPS that provides all of the relevant information about collection protocols. Ten electronic collection protocols were generated using the supplementary Protocol Editor tool, and these protocols were successfully implemented in the existing BIMS. Moreover, an electronic list of collection protocols for the current studies being performed at each institution was included, new collection protocols were added, and the existing protocols were redesigned to be modifiable. The documentation time was significantly reduced after implementing the eSCPS (5 ± 2 min vs. 7 ± 3 min; p = 0.0002). The eSCPS improves the integrity and facilitates the exchange of specimen collection protocols in the existing open-source BIMS.

EPA's approach to regulation of mixed waste and status of future activities

International Nuclear Information System (INIS)

Shackleford, B.

1988-01-01

Regulation of radioactive mixed waste is a topic that has received much attention in the past several years. Much of the discussion and confusion stemmed from uncertainty about applicable regulatory authorities. On July 3, 1986, EPA clarified its position that the Resource Conservation and Recovery Act (RCRA) applied to the hazardous component of radioactive mixed waste. The Agency announced this clarification in the Federal Register and informed States that they must seek authority to regulate mixed waste in order to obtain or maintain RCRA authorization to administer and enforce a hazardous waste program in lieu of EPA. Since that time, five States have received authorization to regulate mixed waste: Colorado, South Carolina, Tennessee, Washington, and Georgia. Authorized States issue RCRA permits in lieu of EPA. Currently, 44 States have been authorized for the base RCRA program, Conversely, 12 States and Trust Territories have no RCRA authorization. In these States and territories, EPA administers that RCRA hazardous waste program. A more stringent State requirement occurs when a State allows less time for compliance than would be provided under Federal law, for example. There is a third authorization category with respect to mixed waste that I have yet to address. This category is made up of States which have EPA authorization to regulate hazardous waste but have yet to obtain mixed waste authorization. Most States fall into this category. In these States, of which there are 39, mixed wastes are not hazardous wastes and subject to Subtitle C regulations

Overview of the EPA quality system for environmental programs

Energy Technology Data Exchange (ETDEWEB)

Johnson, G.L. [Environmental Protection Agency, Research Triangle Park, NC (United States)

1993-12-31

Formalized quality assurance program requirements for the U.S. Environmental Protection Agency (EPA) have been established for more than a decade. During this period, the environmental issues and concerns addressed by the EPA have changed. Many issues, such as ozone depletion and global climate warming, have become international concerns among the world environmental community. Other issues, such as hazardous waste cleanup and clean air, remain a focus of national environmental concerns. As the environmental issues of the 1980`s evolved, the traditional quality assurance (QA) program was transformed through the use of quality management principles into a Quality System to help managers meet the needs of the 1990`s and beyond.

Fundamental changes to EPA's research enterprise: the path forward.

Science.gov (United States)

Anastas, Paul T

2012-01-17

Environmental protection in the United States has reached a critical juncture. It has become clear that to address the complex and interrelated environmental challenges we face, we must augment our traditional approaches. The scientific community must build upon its deep understanding of risk assessment, risk management, and reductionism with tools, technologies, insights and approaches to pursue sustainability. The U.S. Environmental Protection Agency (EPA) has recognized this need for systemic change by implementing a new research paradigm called "The Path Forward." This paper outlines the principles of the Path Forward and the actions taken since 2010 to align EPA's research efforts with the goal of sustainability.

Overview of the EPA quality system for environmental programs

International Nuclear Information System (INIS)

Johnson, G.L.

1993-01-01

Formalized quality assurance program requirements for the U.S. Environmental Protection Agency (EPA) have been established for more than a decade. During this period, the environmental issues and concerns addressed by the EPA have changed. Many issues, such as ozone depletion and global climate warming, have become international concerns among the world environmental community. Other issues, such as hazardous waste cleanup and clean air, remain a focus of national environmental concerns. As the environmental issues of the 1980's evolved, the traditional quality assurance (QA) program was transformed through the use of quality management principles into a Quality System to help managers meet the needs of the 1990's and beyond

Guide to Considering Children's Health When Developing EPA Actions: Implementing Executive Order 13045 and EPA's Policy on Evaluating Health Risks to Children

Science.gov (United States)

Recommendations on integrating children's health considerations into EPA's Action Development Process (ADP). Also how to identify economically significant actions, disproportionate risk, and developing the Analytical Blueprint.

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

Science.gov (United States)

EPA Method 1615 measures enteroviruses and noroviruses present in environmental and drinking waters. The viral ribonucleic acid (RNA) from water sample concentrates is extracted and tested for enterovirus and norovirus RNA using reverse transcription-quantitative PCR (RT-qPCR). V...

Chronic Psychological Stress Was Not Ameliorated by Omega-3 Eicosapentaenoic Acid (EPA

Directory of Open Access Journals (Sweden)

Joanne Bradbury

2017-10-01

Full Text Available Background: Chronic psychological stress and mental health disorders are endemic in Western culture where population dietary insufficiencies of omega-3 fatty acids (n-3FA from seafood have been observed.Objective: This study was designed to test for a causal relationship between one of the most active components of fish oil, eicosapentaenoic acid (EPA, and chronic psychological stress.Method: A randomized double-blind, placebo-controlled clinical trial with parallel-assignment to two groups was designed (Trial Id: ACTRN12610000404022. The interventions were four EPA-rich fish oil capsules per day, delivering 2.2 g/d EPA (and 0.44 g/d DHA, or identical placebo (low-phenolic olive oil capsules with 5% fish oil to aid blinding. The primary outcome was the between-group difference on the Perceived Stress Scale (PSS-10 after 12 weeks supplementation. An a priori power analysis determined that group sizes of 43 would provide 80% power to detect a significant between-group difference of 12.5%, at α = 0.05. Ninety community members (64 females, 26 males reporting chronic work stress were recruited via public advertising in northern NSW, Australia.Results: At baseline the omega-3 index (EPA + DHA as % to total fatty acids in red blood cell membranes was 5.2% in both groups (SD = 1.6% control group; 1.8% active group. After supplementation this remained stable at 5.3% (SD = 1.6% for the control group but increased to 8.9% (SD = 1.5% for the active group, demonstrating successful incorporation of EPA into cells. Intention-to-treat (ITT analysis found no significant between-group differences in PSS outcome scores post-intervention (b = 1.21, p = 0.30 after adjusting for sex (b = 2.36, p = 0.079, baseline PSS (b = 0.42, p = 0.001 and baseline logEPA [b = 1.41, p = 0.185; F(3, 86 = 8.47, p < 0.01, n = 89, R-square = 0.243].Discussion: Treatment increased cell membrane EPA but, contrary to the hypothesis, there was no effect on perceived stress. Limitations

Mac protocols for wireless sensor network (wsn): a comparative study

International Nuclear Information System (INIS)

Arshad, J.; Akram, Q.; Saleem, Y.

2014-01-01

Data communication between nodes is carried out under Medium Access Control (MAC) protocol which is defined at data link layer. The MAC protocols are responsible to communicate and coordinate between nodes according to the defined standards in WSN (Wireless Sensor Networks). The design of a MAC protocol should also address the issues of energy efficiency and transmission efficiency. There are number of MAC protocols that exist in the literature proposed for WSN. In this paper, nine MAC protocols which includes S-MAC, T-MAC, Wise-MAC, Mu-MAC, Z-MAC, A-MAC, D-MAC, B-MAC and B-MAC+ for WSN have been explored, studied and analyzed. These nine protocols are classified in contention based and hybrid (combination of contention and schedule based) MAC protocols. The goal of this comparative study is to provide a basis for MAC protocols and to highlight different mechanisms used with respect to parameters for the evaluation of energy and transmission efficiency in WSN. This study also aims to give reader a better understanding of the concepts, processes and flow of information used in these MAC protocols for WSN. A comparison with respect to energy reservation scheme, idle listening avoidance, latency, fairness, data synchronization, and throughput maximization has been presented. It was analyzed that contention based MAC protocols are less energy efficient as compared to hybrid MAC protocols. From the analysis of contention based MAC protocols in term of energy consumption, it was being observed that protocols based on preamble sampling consume lesser energy than protocols based on static or dynamic sleep schedule. (author)

Two mini-preparation protocols to DNA extraction from plants with ...

African Journals Online (AJOL)

AJB SERVER

2006-10-16

Oct 16, 2006 ... samples to process and it is also a non expensive protocol. This method also ... because many of those chemicals inhibit PCR reactions. (Pandey et al., 1996) ... Spin at 15,000 rpm for 15 min and wash the DNA pellet with 1.2 ml ... Protocol: To 200 mg frozen and ground tissue plant material, add 900 µl of.

Independent Quality Control Review of EPA OIG Operations

Science.gov (United States)

The review considers selected audit work performed by EPA OIG, Office of Audit, Congressional and Public Liaison, Mission Systems, and Program Evaluation, during the period for 1 October 2006 through 30 September 2007 .

BIOREMEDIATION OF HAZARDOUS WASTE SITES: PRACTICAL APPROACHES TO IMPLEMENTATION (EPA/625/K-96/001)

Science.gov (United States)

This document contains abstracts and slide hardcopy for the U.S. Environmental Protection Agency's (EPA's) "Seminar Series on Bioremediation of Hazardous Waste Sites: Practical Approaches to Implementation." This technology transfer seminar series, sponsored by EPA's Biosystems ...

Meet EPA Environmental Engineer Terra Haxton, Ph.D.

Science.gov (United States)

EPA Environmental Engineer Terra Haxton, Ph.D., uses computer simulation models to protect drinking water. She investigates approaches to help water utilities be better prepared to respond to contamination incidents in their distribution systems.

EPA Region 1 - New England Towns, with Population

Data.gov (United States)

U.S. Environmental Protection Agency — The New England Town Boundary coverage is a compilation of coverages received from the six New England State GIS Offices. The EPA New England GIS Center appended the...

Eicosapentaenoic acid (EPA) induced apoptosis in HepG2 cells through ROS–Ca{sup 2+}–JNK mitochondrial pathways

Energy Technology Data Exchange (ETDEWEB)

Zhang, Yuanyuan; Han, Lirong [Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economy Technological Development Area, Tianjin 300457 (China); Qi, Wentao [Academy of State Administration of Grain, No.11 Baiwanzhuang Avenue, Xicheng District, Beijing, 100037 (China); Cheng, Dai; Ma, Xiaolei; Hou, Lihua [Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economy Technological Development Area, Tianjin 300457 (China); Cao, Xiaohong, E-mail: caoxh@tust.edu.cn [Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economy Technological Development Area, Tianjin 300457 (China); Wang, Chunling, E-mail: wangchunling@tust.edu.cn [Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economy Technological Development Area, Tianjin 300457 (China)

2015-01-24

Highlights: • EPA evoked ROS formation, [Ca{sup 2+}]{sub c} accumulation, the opening of MPTP and the phosphorylation of JNK. • EPA-induced [Ca{sup 2+}]{sub c} elevation was depended on production of ROS. • EPA-induced ROS generation, [Ca{sup 2+}]{sub c} increase, and JNK activated caused MPTP opening. • The apoptosis induced by EPA was related to release of cytochrome C through the MPTP. • EPA induced HepG2 cells apoptosis through ROS–Ca{sup 2+}–JNK mitochondrial pathways. - Abstract: Eicosapentaenoic acid (EPA), a well-known dietary n−3 PUFAS, has been considered to inhibit proliferation of tumor cells. However, the molecular mechanism related to EPA-induced liver cancer cells apoptosis has not been reported. In this study, we investigated the effect of EPA on HepG2 cells proliferation and apoptosis mechanism through mitochondrial pathways. EPA inhibited proliferation of HepG2 cells in a dose-dependent manner and had no significant effect on the cell viability of humor normal liver L-02 cells. It was found that EPA initially evoked ROS formation, leading to [Ca{sup 2+}]{sub c} accumulation and the mitochondrial permeability transition pore (MPTP) opening; EPA-induced HepG2 cells apoptosis was inhibited by N-acetylcysteine (NAC, an inhibitor of ROS), 1,2-bis (2-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid (BAPTA-AM, a chelator of calcium) and CsA (inhibitor of MPTP). The relationship between ROS production, the increase of cytoplasmic Ca and MPTP opening was detected. It seems that ROS may act as an upstream regulator of EPA-induced [Ca{sup 2+}]{sub c} generation, moreover, generation of ROS, overload of mitochondrial [Ca{sup 2+}]{sub c}, and JNK activated cause the opening of MPTP. Western blotting results showed that EPA elevated the phosphorylation status of JNK, processes associated with the ROS generation. Simultaneously, the apoptosis induced by EPA was related to release of cytochrome C from mitochondria to cytoplasm through the MPTP

PROTOCOL FOR EXAMINATION OF THE INNER CAN CLOSURE WELD REGION FOR 3013 DE CONTAINERS

Energy Technology Data Exchange (ETDEWEB)

Mickalonis, J.

2014-09-16

The protocol for the examination of the inner can closure weld region (ICCWR) for 3013 DE containers is presented within this report. The protocol includes sectioning of the inner can lid section, documenting the surface condition, measuring corrosion parameters, and storing of samples. This protocol may change as the investigation develops since findings may necessitate additional steps be taken. Details of the previous analyses, which formed the basis for this protocol, are also presented.

Endangered Species Case - Washington Toxics Coalition v. EPA

Science.gov (United States)

This Web page provides information on the Washington Toxics Coalition v. EPA case, related to protection of Pacific salmon and steelhead, and links to the biological opinions issued by the NMFS and EPA’s responses.

40 CFR 59.210 - Addresses of EPA Regional Offices.

Science.gov (United States)

2010-07-01

..., Kentucky, Mississippi, North Carolina, South Carolina, Tennessee), Director, Air, Pesticides, and Toxics...-3507. EPA Region VI (Arkansas, Louisiana, New Mexico, Oklahoma, Texas), Director, Multimedia Planning...

GAC-EPA

CERN Multimedia

GAC-EPA

2013-01-01

En tant que Président du GAC-EPA, je porte à votre connaissance ce communiqué émanant de la Direction du CERN. Le 2 juin 2013, le CERN inaugure le projet Passeport Big Bang, un parcours touristique et scientifique formé de dix plates-formes d'exposition devant dix sites du CERN dans le Pays de Gex et le Canton de Genève. Les plateformes sont reliées par des itinéraires balisés et par un jeu de piste. C'est un projet est mené en collaboration avec les communes du Pays de Gex, Meyrin et Genève Tourisme dans un souci de renforcer notre dialogue avec nos voisins : http://passeport-big-bang.web.cern.ch/fr. A l’occasion de cette inauguration, nous organisons un événement populaire et festif : le matin, les familles pourront participer à des randonnées à vélo tandis que les sportifs pourront tester les 5...

78 FR 9387 - Notice of Availability for Public Review and Comment: Draft EPA Climate Change Adaptation Plan

Science.gov (United States)

2013-02-08

... identifying and responding to the challenges that a changing climate poses to human health and the environment... fulfill its mission of protecting human health and the environment even as the climate changes. EPA...: Draft EPA Climate Change Adaptation Plan AGENCY: Environmental Protection Agency (EPA). ACTION: Notice...