WorldWideScience

Sample records for enzyme-based microfluidic biofuel

  1. Microfluidic glycosyl hydrolase screening for biomass-to-biofuel conversion.

    Science.gov (United States)

    Bharadwaj, Rajiv; Chen, Zhiwei; Datta, Supratim; Holmes, Bradley M; Sapra, Rajat; Simmons, Blake A; Adams, Paul D; Singh, Anup K

    2010-11-15

    The hydrolysis of biomass to fermentable sugars using glycosyl hydrolases such as cellulases and hemicellulases is a limiting and costly step in the conversion of biomass to biofuels. Enhancement in hydrolysis efficiency is necessary and requires improvement in both enzymes and processing strategies. Advances in both areas in turn strongly depend on the progress in developing high-throughput assays to rapidly and quantitatively screen a large number of enzymes and processing conditions. For example, the characterization of various cellodextrins and xylooligomers produced during the time course of saccharification is important in the design of suitable reactors, enzyme cocktail compositions, and biomass pretreatment schemes. We have developed a microfluidic-chip-based assay for rapid and precise characterization of glycans and xylans resulting from biomass hydrolysis. The technique enables multiplexed separation of soluble cellodextrins and xylose oligomers in around 1 min (10-fold faster than HPLC). The microfluidic device was used to elucidate the mode of action of Tm_Cel5A, a novel cellulase from hyperthermophile Thermotoga maritima . The results demonstrate that the cellulase is active at 80 °C and effectively hydrolyzes cellodextrins and ionic-liquid-pretreated switchgrass and Avicel to glucose, cellobiose, and cellotriose. The proposed microscale approach is ideal for quantitative large-scale screening of enzyme libraries for biomass hydrolysis, for development of energy feedstocks, and for polysaccharide sequencing.

  2. Glucose oxidase as a biocatalytic enzyme-based bio-fuel cell using Nafion membrane limiting crossover

    International Nuclear Information System (INIS)

    Naidoo, S; Blottnitz, H; Naidoo, Q; Vaivars, G

    2013-01-01

    A novel combination for an Enzyme-based Biofuel cell included a Nafion membrane as an ion transporter that maintained a working cell charge and inhibited membrane degradation. The prototype cell chamber used oxygen (O 2 ) in the cathode cell and glucose in the anode. The Nafion membrane stability studied here was evidently in the region of 0% loss of conductivity as the charge was constant and increased after the addition of glucose. The prototype cell chamber used NaCl in the cathode cell and glucose oxidase (GOx) in the anodic chamber was successfully studied for membrane stability showed in this study no evidence of poisoning from membrane leakage in a controlled pH environment. There was no crossover at the anaerobic operating ambient temperatures and under physiological pH 5 – 7 conditions. In this research we have successfully used a Nafion membrane together with GOx and under controlled conditions produced respectable power densities

  3. Microbial surface displayed enzymes based biofuel cell utilizing degradation products of lignocellulosic biomass for direct electrical energy.

    Science.gov (United States)

    Fan, Shuqin; Hou, Chuantao; Liang, Bo; Feng, Ruirui; Liu, Aihua

    2015-09-01

    In this work, a bacterial surface displaying enzyme based two-compartment biofuel cell for the direct electrical energy conversion from degradation products of lignocellulosic biomass is reported. Considering that the main degradation products of the lignocellulose are glucose and xylose, xylose dehydrogenase (XDH) displayed bacteria (XDH-bacteria) and glucose dehydrogenase (GDH) displayed bacteria (GDH-bacteria) were used as anode catalysts in anode chamber with methylene blue as electron transfer mediator. While the cathode chamber was constructed with laccase/multi-walled-carbon nanotube/glassy-carbon-electrode. XDH-bacteria exhibited 1.75 times higher catalytic efficiency than GDH-bacteria. This assembled enzymatic fuel cell exhibited a high open-circuit potential of 0.80 V, acceptable stability and energy conversion efficiency. Moreover, the maximum power density of the cell could reach 53 μW cm(-2) when fueled with degradation products of corn stalk. Thus, this finding holds great potential to directly convert degradation products of biomass into electrical energy. Copyright © 2015 Elsevier Ltd. All rights reserved.

  4. Stack air-breathing membraneless glucose microfluidic biofuel cell

    International Nuclear Information System (INIS)

    Galindo-de-la-Rosa, J; Moreno-Zuria, A; Vallejo-Becerra, V; Guerra-Balcázar, M; Ledesma-García, J; Arjona, N; Arriaga, L G

    2016-01-01

    A novel stacked microfluidic fuel cell design comprising re-utilization of the anodic and cathodic solutions on the secondary cell is presented. This membraneless microfluidic fuel cell employs porous flow-through electrodes in a “V”-shape cell architecture. Enzymatic bioanodic arrays based on glucose oxidase were prepared by immobilizing the enzyme onto Toray carbon paper electrodes using tetrabutylammonium bromide, Nafion and glutaraldehyde. These electrodes were characterized through the scanning electrochemical microscope technique, evidencing a good electrochemical response due to the electronic transference observed with the presence of glucose over the entire of the electrode. Moreover, the evaluation of this microfluidic fuel cell with an air-breathing system in a double-cell mode showed a performance of 0.8951 mWcm -2 in a series connection (2.2822mAcm -2 , 1.3607V), and 0.8427 mWcm -2 in a parallel connection (3.5786mAcm -2 , 0.8164V). (paper)

  5. Biofuels

    International Nuclear Information System (INIS)

    Poitrat, E.

    2009-01-01

    Biofuels are fuels made from non-fossil vegetal or animal materials (biomass). They belong to the renewable energy sources as they do not contribute to worsen some global environmental impacts, like the greenhouse effect, providing that their production is performed in efficient energy conditions with low fossil fuel consumption. This article presents: 1 - the usable raw materials: biomass-derived resources, qualitative and quantitative aspects, biomass uses; 2 - biofuels production from biomass: alcohols and ethers, vegetable oils and their esters, synthetic liquid or gaseous biofuels, biogas; 3 - characteristics of liquid biofuels and comparison with gasoline and diesel fuel; 4 - biofuel uses: alcohols and their esters, biofuels with oxygenated compounds; vegetable oils and their derivatives in diesel engines, biogas, example of global environmental impact: the greenhouse effect. (J.S.)

  6. Biofuel Database

    Science.gov (United States)

    Biofuel Database (Web, free access)   This database brings together structural, biological, and thermodynamic data for enzymes that are either in current use or are being considered for use in the production of biofuels.

  7. Biofuels combustion.

    Science.gov (United States)

    Westbrook, Charles K

    2013-01-01

    This review describes major features of current research in renewable fuels derived from plants and from fatty acids. Recent and ongoing fundamental studies of biofuel molecular structure, oxidation reactions, and biofuel chemical properties are reviewed, in addition to combustion applications of biofuels in the major types of engines in which biofuels are used. Biofuels and their combustion are compared with combustion features of conventional petroleum-based fuels. Two main classes of biofuels are described, those consisting of small, primarily alcohol, fuels (particularly ethanol, n-butanol, and iso-pentanol) that are used primarily to replace or supplement gasoline and those derived from fatty acids and used primarily to replace or supplement conventional diesel fuels. Research efforts on so-called second- and third-generation biofuels are discussed briefly.

  8. Biofuels worldwide

    International Nuclear Information System (INIS)

    His, St.

    2004-01-01

    After over 20 years of industrial development, the outlook for biofuels now looks bright. Recent developments indicate that the use of biofuels, previously confined to a handful of countries including Brazil and the United States, is 'going global' and a world market may emerge. However, these prospects could eventually be limited by constraints relative to resources and costs. The future of biofuels probably depends on the development of new technologies to valorize lignocellulosic substances such as wood and straw. (author)

  9. Biofuels barometer

    International Nuclear Information System (INIS)

    Anon.

    2008-01-01

    Biofuels represent 2,6% of the energy content of all the fuels used in road transport in Europe today. Nearly half of the target of 5,75% for 2010 set by the directive on biofuels has thus been reached in four years time. To achieve 5,75%, the european union is going to have to increase its production and doubtless call even more on imports, at a moment when biofuels are found at the core of complex ecological and economic issues. This analysis provided data and reflexions on the biofuels situation in the european union: consumption, bio-diesel, bio-ethanol, producers, environmental problems, directives. (A.L.B.)

  10. Microfluidic electronics.

    Science.gov (United States)

    Cheng, Shi; Wu, Zhigang

    2012-08-21

    Microfluidics, a field that has been well-established for several decades, has seen extensive applications in the areas of biology, chemistry, and medicine. However, it might be very hard to imagine how such soft microfluidic devices would be used in other areas, such as electronics, in which stiff, solid metals, insulators, and semiconductors have previously dominated. Very recently, things have radically changed. Taking advantage of native properties of microfluidics, advances in microfluidics-based electronics have shown great potential in numerous new appealing applications, e.g. bio-inspired devices, body-worn healthcare and medical sensing systems, and ergonomic units, in which conventional rigid, bulky electronics are facing insurmountable obstacles to fulfil the demand on comfortable user experience. Not only would the birth of microfluidic electronics contribute to both the microfluidics and electronics fields, but it may also shape the future of our daily life. Nevertheless, microfluidic electronics are still at a very early stage, and significant efforts in research and development are needed to advance this emerging field. The intention of this article is to review recent research outcomes in the field of microfluidic electronics, and address current technical challenges and issues. The outlook of future development in microfluidic electronic devices and systems, as well as new fabrication techniques, is also discussed. Moreover, the authors would like to inspire both the microfluidics and electronics communities to further exploit this newly-established field.

  11. Biofuels barometer

    International Nuclear Information System (INIS)

    Anon.

    2011-01-01

    In 2010 bio-fuel continued to gnaw away at petrol and diesel consumption in the European Union (EU). However its pace backs the assertion that bio-fuel consumption growth in EU slackened off in 2010. In the transport sector, it increased by only 1.7 Mtoe compared to 2.7 Mtoe in 2009. The final total bio-fuel consumption figure for 2010 should hover at around 13.9 Mtoe that can be broken down into 10.7 Mtoe for bio-diesel, 2.9 Mtoe for bio-ethanol and 0.3 Mtoe for others. Germany leads the pack for the consumption of bio-fuels and for the production of bio-diesel followed by France and Spain

  12. Biofuels sources, biofuel policy, biofuel economy and global biofuel projections

    International Nuclear Information System (INIS)

    Demirbas, Ayhan

    2008-01-01

    The term biofuel is referred to liquid, gas and solid fuels predominantly produced from biomass. Biofuels include energy security reasons, environmental concerns, foreign exchange savings, and socioeconomic issues related to the rural sector. Biofuels include bioethanol, biomethanol, vegetable oils, biodiesel, biogas, bio-synthetic gas (bio-syngas), bio-oil, bio-char, Fischer-Tropsch liquids, and biohydrogen. Most traditional biofuels, such as ethanol from corn, wheat, or sugar beets, and biodiesel from oil seeds, are produced from classic agricultural food crops that require high-quality agricultural land for growth. Bioethanol is a petrol additive/substitute. Biomethanol can be produced from biomass using bio-syngas obtained from steam reforming process of biomass. Biomethanol is considerably easier to recover than the bioethanol from biomass. Ethanol forms an azeotrope with water so it is expensive to purify the ethanol during recovery. Methanol recycles easier because it does not form an azeotrope. Biodiesel is an environmentally friendly alternative liquid fuel that can be used in any diesel engine without modification. There has been renewed interest in the use of vegetable oils for making biodiesel due to its less polluting and renewable nature as against the conventional petroleum diesel fuel. Due to its environmental merits, the share of biofuel in the automotive fuel market will grow fast in the next decade. There are several reasons for biofuels to be considered as relevant technologies by both developing and industrialized countries. Biofuels include energy security reasons, environmental concerns, foreign exchange savings, and socioeconomic issues related to the rural sector. The biofuel economy will grow rapidly during the 21st century. Its economy development is based on agricultural production and most people live in the rural areas. In the most biomass-intensive scenario, modernized biomass energy contributes by 2050 about one half of total energy

  13. Algal biofuels.

    Science.gov (United States)

    Razeghifard, Reza

    2013-11-01

    The world is facing energy crisis and environmental issues due to the depletion of fossil fuels and increasing CO2 concentration in the atmosphere. Growing microalgae can contribute to practical solutions for these global problems because they can harvest solar energy and capture CO2 by converting it into biofuel using photosynthesis. Microalgae are robust organisms capable of rapid growth under a variety of conditions including in open ponds or closed photobioreactors. Their reduced biomass compounds can be used as the feedstock for mass production of a variety of biofuels. As another advantage, their ability to accumulate or secrete biofuels can be controlled by changing their growth conditions or metabolic engineering. This review is aimed to highlight different forms of biofuels produced by microalgae and the approaches taken to improve their biofuel productivity. The costs for industrial-scale production of algal biofuels in open ponds or closed photobioreactors are analyzed. Different strategies for photoproduction of hydrogen by the hydrogenase enzyme of green algae are discussed. Algae are also good sources of biodiesel since some species can make large quantities of lipids as their biomass. The lipid contents for some of the best oil-producing strains of algae in optimized growth conditions are reviewed. The potential of microalgae for producing petroleum related chemicals or ready-make fuels such as bioethanol, triterpenic hydrocarbons, isobutyraldehyde, isobutanol, and isoprene from their biomass are also presented.

  14. Biofuels barometer

    International Nuclear Information System (INIS)

    Anon.

    2012-01-01

    The European Union governments no longer view the rapid increase in biofuel consumption as a priority. Between 2010 and 2011 biofuel consumption increased by only 3%, which translates into 13.6 million tonnes of oil equivalent (toe) used in 2011 compared to 13.2 million toe in 2010. In 2011 6 European countries had a biofuel consumption in transport that went further 1 million toe: Germany (2,956,746 toe), France (2,050,873 toe), Spain (1,672,710 toe), Italy (1,432,455 toe), United Kingdom (1,056,105 toe) and Poland (1,017,793 toe). The breakdown of the biofuel consumption for transport in the European Union in 2011 into types of biofuels is: bio-diesel (78%), bio-ethanol (21%), biogas (0.5%) and vegetable oil (0.5%). In 2011, 4 bio-diesel producers had a production capacity in Europe that passed beyond 900,000 tonnes: Diester Industrie International (France) with 3,000,000 tonnes, Neste Oil (Finland) with 1,180,000 tonnes, ADM bio-diesel (Germany) with 975,000 tonnes, and Infinita (Spain) with 900,000 tonnes. It seems that the European Union's attention has shifted to setting up sustainability systems to verify that the biofuel used in the various countries complies with the Renewable Energy Directive's sustainability criteria

  15. Theoretical microfluidics

    DEFF Research Database (Denmark)

    Bruus, Henrik

    Microfluidics is a young and rapidly expanding scientific discipline, which deals with fluids and solutions in miniaturized systems, the so-called lab-on-a-chip systems. It has applications in chemical engineering, pharmaceutics, biotechnology and medicine. As the lab-on-a-chip systems grow...

  16. Fuelling biofuel

    International Nuclear Information System (INIS)

    Collison, M.

    2006-01-01

    The Canadian government has recently committed to legislation ensuring that all transportation fuels will be supplemented with biofuels by 2010. This article provided details of a position paper written by the Canadian Renewable Fuels Association in response to the legislation. Details of new research to optimize the future biodiesel industry were also presented. Guiding principles of the paper included the creation of open markets across provincial boundaries; the manipulation of tax structures to make products competitive in the United States; and establishing quality standards via the Canadian General Standards Board. It is expected that the principles will reassure petroleum producers and retailers, as ethanol behaves differently than gasoline in storage tanks. As ethanol is water-absorbing, retailers must flush and vacuum their tanks to remove water, then install 10 micron filters to protect fuel lines and dispenser filters from accumulated gasoline residue loosened by the ethanol. Refineries are concerned that the average content of ethanol remains consistent across the country, as refiners will be reluctant to make different blends for different provinces. Critics of biodiesel claim that it is not energy-intensive enough to meet demand, and biodiesel crops are not an efficient use of soils that could otherwise be used to grow food crops. However, researchers in Saskatchewan are committed to using a variety of methods such as reduced tillage systems to make biodiesel production more efficient. Laboratory research has resulted in improved refining processes and genetic manipulation of potential biodiesel crops. Membrane technology is now being used to select water from ethanol. A process developed by the Ottawa company Iogen Corporation uses enzymatic hydrolysis to break down the tough fibres found in corn stalks, leaves, wood and other biomass into sugars. Scientists are also continuing to improve oil content yields in canola and soybean crops. It was

  17. Microfluidic Device

    Science.gov (United States)

    Tai, Yu-Chong (Inventor); Zheng, Siyang (Inventor); Lin, Jeffrey Chun-Hui (Inventor); Kasdan, Harvey L. (Inventor)

    2017-01-01

    Described herein are particular embodiments relating to a microfluidic device that may be utilized for cell sensing, counting, and/or sorting. Particular aspects relate to a microfabricated device that is capable of differentiating single cell types from dense cell populations. One particular embodiment relates a device and methods of using the same for sensing, counting, and/or sorting leukocytes from whole, undiluted blood samples.

  18. Enzyme-Based Logic Gates and Networks with Output Signals Analyzed by Various Methods.

    Science.gov (United States)

    Katz, Evgeny

    2017-07-05

    The paper overviews various methods that are used for the analysis of output signals generated by enzyme-based logic systems. The considered methods include optical techniques (optical absorbance, fluorescence spectroscopy, surface plasmon resonance), electrochemical techniques (cyclic voltammetry, potentiometry, impedance spectroscopy, conductivity measurements, use of field effect transistor devices, pH measurements), and various mechanoelectronic methods (using atomic force microscope, quartz crystal microbalance). Although each of the methods is well known for various bioanalytical applications, their use in combination with the biomolecular logic systems is rather new and sometimes not trivial. Many of the discussed methods have been combined with the use of signal-responsive materials to transduce and amplify biomolecular signals generated by the logic operations. Interfacing of biocomputing logic systems with electronics and "smart" signal-responsive materials allows logic operations be extended to actuation functions; for example, stimulating molecular release and switchable features of bioelectronic devices, such as biofuel cells. The purpose of this review article is to emphasize the broad variability of the bioanalytical systems applied for signal transduction in biocomputing processes. All bioanalytical systems discussed in the article are exemplified with specific logic gates and multi-gate networks realized with enzyme-based biocatalytic cascades. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. Biofuel technologies. Recent developments

    Energy Technology Data Exchange (ETDEWEB)

    Gupta, Vijai Kumar [National Univ. of Ireland Galway (Ireland). Dept. of Biochemistry; MITS Univ., Rajasthan (India). Dept. of Science; Tuohy, Maria G. (eds.) [National Univ. of Ireland Galway (Ireland). Dept. of Biochemistry

    2013-02-01

    Written by experts. Richly illustrated. Of interest to both experienced researchers and beginners in the field. Biofuels are considered to be the main potential replacement for fossil fuels in the near future. In this book international experts present recent advances in biofuel research and related technologies. Topics include biomethane and biobutanol production, microbial fuel cells, feedstock production, biomass pre-treatment, enzyme hydrolysis, genetic manipulation of microbial cells and their application in the biofuels industry, bioreactor systems, and economical processing technologies for biofuel residues. The chapters provide concise information to help understand the technology-related implications of biofuels development. Moreover, recent updates on biofuel feedstocks, biofuel types, associated co- and byproducts and their applications are highlighted. The book addresses the needs of postgraduate researchers and scientists across diverse disciplines and industrial sectors in which biofuel technologies and related research and experimentation are pursued.

  20. Panorama 2007: Biofuels Worldwide

    International Nuclear Information System (INIS)

    Prieur-Vernat, A.; His, St.

    2007-01-01

    The biofuels market is booming: after more than 20 years of industrial development, global bio-fuel production is growing fast. Willingness to reduce their oil dependence and necessity to promote low-carbon energies are the two main drivers for states to support biofuels development. (author)

  1. Microfluidic interconnects

    Science.gov (United States)

    Benett, William J.; Krulevitch, Peter A.

    2001-01-01

    A miniature connector for introducing microliter quantities of solutions into microfabricated fluidic devices. The fluidic connector, for example, joins standard high pressure liquid chromatography (HPLC) tubing to 1 mm diameter holes in silicon or glass, enabling ml-sized volumes of sample solutions to be merged with .mu.l-sized devices. The connector has many features, including ease of connect and disconnect; a small footprint which enables numerous connectors to be located in a small area; low dead volume; helium leak-tight; and tubing does not twist during connection. Thus the connector enables easy and effective change of microfluidic devices and introduction of different solutions in the devices.

  2. Limits to biofuels

    Directory of Open Access Journals (Sweden)

    Johansson S.

    2013-06-01

    Full Text Available Biofuel production is dependent upon agriculture and forestry systems, and the expectations of future biofuel potential are high. A study of the global food production and biofuel production from edible crops implies that biofuel produced from edible parts of crops lead to a global deficit of food. This is rather well known, which is why there is a strong urge to develop biofuel systems that make use of residues or products from forest to eliminate competition with food production. However, biofuel from agro-residues still depend upon the crop production system, and there are many parameters to deal with in order to investigate the sustainability of biofuel production. There is a theoretical limit to how much biofuel can be achieved globally from agro-residues and this amounts to approximately one third of todays’ use of fossil fuels in the transport sector. In reality this theoretical potential may be eliminated by the energy use in the biomass-conversion technologies and production systems, depending on what type of assessment method is used. By surveying existing studies on biofuel conversion the theoretical limit of biofuels from 2010 years’ agricultural production was found to be either non-existent due to energy consumption in the conversion process, or up to 2–6000TWh (biogas from residues and waste and ethanol from woody biomass in the more optimistic cases.

  3. Limits to biofuels

    Science.gov (United States)

    Johansson, S.

    2013-06-01

    Biofuel production is dependent upon agriculture and forestry systems, and the expectations of future biofuel potential are high. A study of the global food production and biofuel production from edible crops implies that biofuel produced from edible parts of crops lead to a global deficit of food. This is rather well known, which is why there is a strong urge to develop biofuel systems that make use of residues or products from forest to eliminate competition with food production. However, biofuel from agro-residues still depend upon the crop production system, and there are many parameters to deal with in order to investigate the sustainability of biofuel production. There is a theoretical limit to how much biofuel can be achieved globally from agro-residues and this amounts to approximately one third of todays' use of fossil fuels in the transport sector. In reality this theoretical potential may be eliminated by the energy use in the biomass-conversion technologies and production systems, depending on what type of assessment method is used. By surveying existing studies on biofuel conversion the theoretical limit of biofuels from 2010 years' agricultural production was found to be either non-existent due to energy consumption in the conversion process, or up to 2-6000TWh (biogas from residues and waste and ethanol from woody biomass) in the more optimistic cases.

  4. Biofuels Baseline 2008

    Energy Technology Data Exchange (ETDEWEB)

    Hamelinck, C.; Koper, M.; Berndes, G.; Englund, O.; Diaz-Chavez, R.; Kunen, E.; Walden, D.

    2011-10-15

    The European Union is promoting the use of biofuels and other renewable energy in transport. In April 2009, the Renewable Energy Directive (2009/28/EC) was adopted that set a 10% target for renewable energy in transport in 2020. The directive sets several requirements to the sustainability of biofuels marketed in the frame of the Directive. The Commission is required to report to the European Parliament on a regular basis on a range of sustainability impacts resulting from the use of biofuels in the EU. This report serves as a baseline of information for regular monitoring on the impacts of the Directive. Chapter 2 discusses the EU biofuels market, the production and consumption of biofuels and international trade. It is derived where the feedstock for EU consumed biofuels originally come from. Chapter 3 discusses the biofuel policy framework in the EU and major third countries of supply. It looks at various policy aspects that are relevant to comply with the EU sustainability requirements. Chapter 4 discusses the environmental and social sustainability aspects associated with EU biofuels and their feedstock. Chapter 5 discusses the macro-economic effects that indirectly result from increased EU biofuels consumption, on commodity prices and land use. Chapter 6 presents country factsheets for main third countries that supplied biofuels to the EU market in 2008.

  5. Life Cycle Assessment for Biofuels

    Science.gov (United States)

    A presentation based on life cycle assessment (LCA) for biofuels is given. The presentation focuses on energy and biofuels, interesting environmental aspects of biofuels, and how to do a life cycle assessment with some examples related to biofuel systems. The stages of a (biofuel...

  6. Biomass, biogas and biofuels

    International Nuclear Information System (INIS)

    Colonna, P.

    2011-01-01

    This article reviews the different ways to produce biofuels. It appears that there are 3 generations of biofuels. The first generation was based on the use of the energetic reserves of the plants for instance sugar from beetroot or starch from cereals or oil from oleaginous plants. The second generation is based on a more complete use of the plant, the main constituents of the plant: cellulose and lignin are turned into energy. The third generation of biofuels relies on the use of energy plants and algae. The second generation of biofuels reduces drastically the competition between an alimentary use and a non-alimentary use of plants. In 2008 the production of biofuels reached 43 Mtep which represents only 2% of all the energy used in the transport sector. The international agency for energy expects that the production of biofuels would be multiplied by a factor 6 (even 10 if inciting measures are taken) by 2030. (A.C.)

  7. The Danish Biofuel Debate

    DEFF Research Database (Denmark)

    Hansen, Janus

    2014-01-01

    of biofuels enrol scientific authority to support their positions? The sociological theory of functional differentiation combined with the concept of advocacy coalition can help in exploring this relationship between scientific claims-making and the policy stance of different actors in public debates about...... biofuels. In Denmark two distinct scientific perspectives about biofuels map onto the policy debates through articulation by two competing advocacy coalitions. One is a reductionist biorefinery perspective originating in biochemistry and neighbouring disciplines. This perspective works upwards from...

  8. Biofuels in China.

    Science.gov (United States)

    Tan, Tianwei; Yu, Jianliang; Lu, Jike; Zhang, Tao

    2010-01-01

    The Chinese government is stimulating the biofuels development to replace partially fossil fuels in the transport sector, which can enhance energy security, reduce greenhouse gas emissions, and stimulate rural development. Bioethanol, biodiesel, biobutanol, biogas, and biohydrogen are the main biofuels developed in China. In this chapter, we mainly present the current status of biofuel development in China, and illustrate the issues of feedstocks, food security and conversion processes.

  9. Microfluidic sieve valves

    Science.gov (United States)

    Quake, Stephen R; Marcus, Joshua S; Hansen, Carl L

    2015-01-13

    Sieve valves for use in microfluidic device are provided. The valves are useful for impeding the flow of particles, such as chromatography beads or cells, in a microfluidic channel while allowing liquid solution to pass through the valve. The valves find particular use in making microfluidic chromatography modules.

  10. Microfluidic Dye Lasers

    DEFF Research Database (Denmark)

    Kristensen, Anders; Balslev, Søren; Gersborg-Hansen, Morten

    2006-01-01

    A technology for miniaturized, polymer based lasers, suitable for integration with planar waveguides and microfluidic networks is presented. The microfluidic dye laser device consists of a microfluidic channel with an embedded optical resonator. The devices are fabricated in a thin polymer film...

  11. NREL biofuels program overview

    Energy Technology Data Exchange (ETDEWEB)

    Mielenz, J.R. [National Renewable Energy Laboratory, Golden, CO (United States)

    1996-09-01

    The NREL Biofuels Program has been developing technology for conversion of biomass to transportation fuels with support from DOE Office of Transportation Technologies Biofuels System Program. This support has gone to both the National Renewable Energy Laboratory, and over 100 subcontractors in universities and industry. This overview will outline the value of the Biofuels development program to the Nation, the current status of the technology development, and what research areas still need further support and progress for the development of a biofuels industry in the US.

  12. Engineering microbes to produce biofuels.

    Science.gov (United States)

    Wackett, Lawrence P

    2011-06-01

    The current biofuels landscape is chaotic. It is controlled by the rules imposed by economic forces and driven by the necessity of finding new sources of energy, particularly motor fuels. The need is bringing forth great creativity in uncovering new candidate fuel molecules that can be made via metabolic engineering. These next generation fuels include long-chain alcohols, terpenoid hydrocarbons, and diesel-length alkanes. Renewable fuels contain carbon derived from carbon dioxide. The carbon dioxide is derived directly by a photosynthetic fuel-producing organism(s) or via intermediary biomass polymers that were previously derived from carbon dioxide. To use the latter economically, biomass depolymerization processes must improve and this is a very active area of research. There are competitive approaches with some groups using enzyme based methods and others using chemical catalysts. With the former, feedstock and end-product toxicity loom as major problems. Advances chiefly rest on the ability to manipulate biological systems. Computational and modular construction approaches are key. For example, novel metabolic networks have been constructed to make long-chain alcohols and hydrocarbons that have superior fuel properties over ethanol. A particularly exciting approach is to implement a direct utilization of solar energy to make a usable fuel. A number of approaches use the components of current biological systems, but re-engineer them for more direct, efficient production of fuels. Copyright © 2010 Elsevier Ltd. All rights reserved.

  13. A roadmap for biofuels...

    NARCIS (Netherlands)

    Faaij, A.P.C.; Londo, H.M.

    2009-01-01

    Biofuels have been in the eye of the storm, in particular since 2008, when the food crisis was considered by many to be caused by the increased production of biofuels. Heavy criticism in public media made various governments, including the European Commission, reconsider their targets and ambitions

  14. Biofuels for sustainable transportation

    Energy Technology Data Exchange (ETDEWEB)

    Neufeld, S.

    2000-05-23

    Biomass is an attractive energy source, and transportation fuels made from biomass offer a number of benefits. Developing the technology to produce and use biofuels will create transportation fuel options that can positively impact the national energy security, the economy, and the environment. Biofuels include ethanol, methanol, biodiesel, biocrude, and methane.

  15. Algal Biofuels | Bioenergy | NREL

    Science.gov (United States)

    biofuels and bioproducts, Algal Research (2016) Process Design and Economics for the Production of Algal cyanobacteria, Nature Plants (2015) Acid-catalyzed algal biomass pretreatment for integrated lipid and nitrogen, we can indefinitely maintain the genetic state of the sample for future research in biofuels

  16. An overview of biofuels

    International Nuclear Information System (INIS)

    Qureshi, I.H.; Ahmad, S.

    2007-01-01

    Biofuels for transport have received considerable attention due to rising oil prices and growing concern about greenhouse gas emissions. Biofuels namely ethanol and esters of fatty acids have the potential to displace a substantial amount of petroleum fuel in the next few decades which will help to conserve fossil fuel resources. Life cycle analyses show that biofuels release lesser amount of greenhouse gases and other air pollutants. Thus biofuels are seen as a pragmatic step towards reducing carbon dioxide emission from transport sector. Biofuels are compatible with petroleum and combustion engines can easily operate with 10% ethanol and 20% biodiesel blended fuel with no modification. However higher concentrations require 'flex-fuel' engines which automatically adjust fuel injection depending upon fuel mix. Biofuels are derived from renewable biomass and can be produced from a variety of feedstocks. The only limiting factors are the availability of cropland, growth of plants and the climate. Countries with warmer climate can get about five times more biofuel crops from each acre of land than cold climate countries. Genetically modified crops and fast growing trees are being developed increase the production of energy crops. (author)

  17. Biofuels and sustainability.

    Science.gov (United States)

    Solomon, Barry D

    2010-01-01

    Interest in liquid biofuels production and use has increased worldwide as part of government policies to address the growing scarcity and riskiness of petroleum use, and, at least in theory, to help mitigate adverse global climate change. The existing biofuels markets are dominated by U.S. ethanol production based on cornstarch, Brazilian ethanol production based on sugarcane, and European biodiesel production based on rapeseed oil. Other promising efforts have included programs to shift toward the production and use of biofuels based on residues and waste materials from the agricultural and forestry sectors, and perennial grasses, such as switchgrass and miscanthus--so-called cellulosic ethanol. This article reviews these efforts and the recent literature in the context of ecological economics and sustainability science. Several common dimensions for sustainable biofuels are discussed: scale (resource assessment, land availability, and land use practices); efficiency (economic and energy); equity (geographic distribution of resources and the "food versus fuel" debate); socio-economic issues; and environmental effects and emissions. Recent proposals have been made for the development of sustainable biofuels criteria, culminating in standards released in Sweden in 2008 and a draft report from the international Roundtable on Sustainable Biofuels. These criteria hold promise for accelerating a shift away from unsustainable biofuels based on grain, such as corn, and toward possible sustainable feedstock and production practices that may be able to meet a variety of social, economic, and environmental sustainability criteria.

  18. Biofuels for transport

    International Nuclear Information System (INIS)

    2004-01-01

    In the absence of strong government policies, the IEA projects that the worldwide use of oil in transport will nearly double between 2000 and 2030, leading to a similar increase in greenhouse gas emissions. Biofuels, such as ethanol, bio-diesel, and other liquid and gaseous fuels, could offer an important alternative to petroleum over this time frame and help reduce atmospheric pollution. This book looks at recent trends in biofuel production and considers what the future might hold if such alternatives were to displace petroleum in transport. The report takes a global perspective on the nascent biofuels industry, assessing regional similarities and differences as well as the cost and benefits of the various initiatives being undertaken around the world. In the short term, conventional biofuel production processes in IEA countries could help reduce oil use and thence greenhouse gas emissions, although the costs may be high. In the longer term, possibly within the next decade, advances in biofuel production and the use of new feedstocks could lead to greater, more cost-effective reductions. Countries such as Brazil are already producing relatively low-cost biofuels with substantial reductions in fossil energy use and greenhouse gas emissions. This book explores the range of options on offer and asks whether a global trade in biofuels should be more rigorously pursued

  19. The biofuels in France

    International Nuclear Information System (INIS)

    2006-04-01

    The biofuels are liquid renewable energies sources resulting from vegetal matters. Today are two channels of biofuels: the ethanol channel for gasoline and the vegetal oils channel for the diesel. In the first part, the document presents the different channels and the energy efficiency of the products. It shows in the second part the advantages for the environment (CO 2 accounting) and for the energy independence. It discusses then the future developments and the projects. The fourth part is devoted to the legislation, regulations, taxes and financial incentives. The last part presents the french petroleum industry actions and attitudes in the framework of the biofuels development. (A.L.B.)

  20. Biofuels: which interest, which perspectives?

    International Nuclear Information System (INIS)

    2006-01-01

    This paper is a synthesis of several studies concerning the production and utilization of bio-fuels: energy balance and greenhouse effect of the various bio-fuel systems; economical analysis and profitability of bio-fuel production; is the valorization of bio-fuel residues and by-products in animal feeding a realistic hypothesis?; assessment of the cost for the community due to tax exemption for bio-fuels

  1. Rapid manufacturing for microfluidics

    CSIR Research Space (South Africa)

    Land, K

    2012-10-01

    Full Text Available for microfluidics K. LAND, S. HUGO, M MBANJWA, L FOURIE CSIR Materials Science and Manufacturing P O Box 395, Pretoria 0001, SOUTH AFRICA Email: kland@csir.co.za INTRODUCTION Microfluidics refers to the manipulation of very small volumes of fluid.... Microfluidics is at the forefront of developing solutions for drug discovery, diagnostics (from glucose tests to malaria and TB testing) and environmental diagnostics (E-coli monitoring of drinking water). In order to quickly implement new designs, a rapid...

  2. Commercialization of microfluidic devices.

    Science.gov (United States)

    Volpatti, Lisa R; Yetisen, Ali K

    2014-07-01

    Microfluidic devices offer automation and high-throughput screening, and operate at low volumes of consumables. Although microfluidics has the potential to reduce turnaround times and costs for analytical devices, particularly in medical, veterinary, and environmental sciences, this enabling technology has had limited diffusion into consumer products. This article analyzes the microfluidics market, identifies issues, and highlights successful commercialization strategies. Addressing niche markets and establishing compatibility with existing workflows will accelerate market penetration. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. Tunable Microfluidic Dye Laser

    DEFF Research Database (Denmark)

    Olsen, Brian Bilenberg; Helbo, Bjarne; Kutter, Jörg Peter

    2003-01-01

    We present a tunable microfluidic dye laser fabricated in SU-8. The tunability is enabled by integrating a microfluidic diffusion mixer with an existing microfluidic dye laser design by Helbo et al. By controlling the relative flows in the mixer between a dye solution and a solvent......, the concentration of dye in the laser cavity can be adjusted, allowing the wavelength to be tuned. Wavelength tuning controlled by the dye concentration was demonstrated with macroscopic dye lasers already in 1971, but this principle only becomes practically applicable by the use of microfluidic mixing...

  4. Biofuels - 5 disturbing questions

    International Nuclear Information System (INIS)

    Legalland, J.P.; Lemarchand, J.L.

    2008-01-01

    Initially considered as the supreme weapon against greenhouse gas emissions, biofuels are today hold responsible to all harms of the Earth: leap of agriculture products price, deforestation, food crisis. Considered some time ago as the perfect clean substitute to petroleum, biofuels are now suspected to have harmful effects on the environment. Should it be just an enormous technical, environmental and human swindle? Should we abandon immediately biofuels to protect the earth and fight the threatening again starvation? Should we wait for the second generation of efficient biofuels, made from non food-derived products and cultivation wastes? This book analyses this delicate debate through 5 main questions: do they starve the world? Are they a clean energy source? Do they contribute to deforestation? Are they economically practicable? Is the second generation ready? (J.S.)

  5. Market possibilities for biofuels

    International Nuclear Information System (INIS)

    Hektor, B.

    1992-01-01

    The market for biofuels in Sweden after introduction of a proposed CO 2 -tax on fossil fuels is forecast. The competition between biofuels, fossil fuels and electricity is described for important market segments such as: Paper industry, Sawmills, Other energy-intensive industry, Power and heat producers, small Heat producers, and for Space heating of one-family houses. A market increase of the use of biofuels is probable for the segment small (district) heating centrals, 10 TWh in the next ten year period and even more during a longer period. Other market segments will not be much affected. An increased use of biofuels in paper and pulp industry will not influence the fuel market, since the increase will happen in the industry's normal lumber purchase. (2 figs., 18 tabs.)

  6. The biofuels, situation, perspectives

    International Nuclear Information System (INIS)

    Acket, C.

    2007-03-01

    The climatic change with the fight against the greenhouse effect gases, sees the development of ''clean'' energy sources. Meanwhile the biofuels remain penalized by their high production cost, the interest is increasing. Facing their development ecologists highlight the environmental and social negative impacts of the development of the biofuels. The author aims to take stock on the techniques and the utilizations. (A.L.B.)

  7. Bio-fuel barometer

    International Nuclear Information System (INIS)

    2015-01-01

    After a year of doubt and decline the consumption of bio-fuel resumed a growth in 2014 in Europe: +6.1% compared to 2013, to reach 14 millions tep (Mtep) that is just below the 2012 peak. This increase was mainly due to bio-diesel. By taking into account the energy content and not the volume, the consumption of bio-diesel represented 79.7% of bio-fuel consumption in 2014, that of bio-ethanol only 19.1% and that of biogas 1%. The incorporating rate of bio-fuels in fuels used for transport were 4.6% in 2013 and 4.9% in 2014. The trend is good and the future of bio-fuel seems clearer as the European Union has set a not-so-bad limit of 7% for first generation bio-fuels in order to take into account the CASI effect. The CASI effect shows that an increase of the consumption of first generation bio-fuels (it means bio-fuels produced from food crops like rape, soy, cereals, sugar beet,...) implies in fact a global increase in greenhouse gas release that is due to a compensation phenomenon. More uncultivated lands (like forests, grasslands, bogs are turned into cultivated lands in order to compensate lands used for bio-fuel production. In most European countries the consumption of bio-diesel increased in 2014 while it was a bad year for the European industry of ethanol because ethanol prices dropped by 16 %. Oil companies are now among the most important producers of bio-diesel in Europe.

  8. Enzyme based soil stabilization for unpaved road construction

    Directory of Open Access Journals (Sweden)

    Renjith Rintu

    2017-01-01

    Full Text Available Enzymes as soil stabilizers have been successfully used in road construction in several countries for the past 30 years. However, research has shown that the successful application of these enzymes is case specific, emphasizing that enzyme performance is dependent on subgrade soil type, condition and the type of enzyme used as the stabilizer. A universal standard or a tool for road engineers to assess the performance of stabilized unbound pavements using well-established enzymes is not available to date. The research aims to produce a validated assessment tool which can be used to predict strength enhancement within a generalized statistical framework. The objective of the present study is to identify new materials for developing the assessment tool which supports enzyme based stabilization, as well as to identify the correct construction sequence for such new materials. A series of characterization tests were conducted on several soil types obtained from proposed construction sites. Having identified the suitable soil type to mix with the enzyme, a trial road construction has been performed to investigate the efficiency of the enzyme stabilization along with the correct construction sequence. The enzyme stabilization has showed significant improvement of the road performance as was evidenced from the test results which were based on site soil obtained before and after stabilization. The research will substantially benefit the road construction industry by not only replacing traditional construction methods with economical/reliable approaches, but also eliminating site specific tests required in current practice of enzyme based road construction.

  9. Microfluidics for chemical processing

    NARCIS (Netherlands)

    Gardeniers, Johannes G.E.

    2006-01-01

    Microfluidic systems, and more specifically, microfluidic chips, have a number of features that make them particularly useful for the study of chemical reactions on-line. The present paper will discuss two examples, the study of fluidic behaviour at high pressures and the excitation and detection of

  10. World Biofuels Study

    Energy Technology Data Exchange (ETDEWEB)

    Alfstad,T.

    2008-10-01

    This report forms part of a project entitled 'World Biofuels Study'. The objective is to study world biofuel markets and to examine the possible contribution that biofuel imports could make to help meet the Renewable Fuel Standard (RFS) of the Energy Independence and Security Act of 2007 (EISA). The study was sponsored by the Biomass Program of the Assistant Secretary for Energy Efficiency and Renewable Energy (EERE), U.S. Department of Energy. It is a collaborative effort among the Office of Policy and International Affairs (PI), Department of Energy and Oak Ridge National Laboratory (ORNL), National Renewable Energy Laboratory (NREL) and Brookhaven National Laboratory (BNL). The project consisted of three main components: (1) Assessment of the resource potential for biofuel feedstocks such as sugarcane, grains, soybean, palm oil and lignocellulosic crops and development of supply curves (ORNL). (2) Assessment of the cost and performance of biofuel production technologies (NREL). (3) Scenario-based analysis of world biofuel markets using the ETP global energy model with data developed in the first parts of the study (BNL). This report covers the modeling and analysis part of the project conducted by BNL in cooperation with PI. The Energy Technology Perspectives (ETP) energy system model was used as the analytical tool for this study. ETP is a 15 region global model designed using the MARKAL framework. MARKAL-based models are partial equilibrium models that incorporate a description of the physical energy system and provide a bottom-up approach to study the entire energy system. ETP was updated for this study with biomass resource data and biofuel production technology cost and performance data developed by ORNL and NREL under Tasks 1 and 2 of this project. Many countries around the world are embarking on ambitious biofuel policies through renewable fuel standards and economic incentives. As a result, the global biofuel demand is expected to grow very

  11. Biofuels and Biotechnology

    Energy Technology Data Exchange (ETDEWEB)

    Mielenz, Jonathan R [ORNL

    2009-01-01

    The world obtains 86% of its energy from fossil fuels, 40% from petroleum, a majority of which goes to the transportation sector (www.IEA.gov). Well-recognized alternatives are fuels derived from renewable sources known as biofuels. There are a number of biofuels useful for transportation fuels, which include ethanol, biobutanol, mixed alcohols, biodiesel, and hydrogen. These biofuels are produced from biologically derived feedstock, almost exclusively being plant materials, either food or feed sources or inedible plant material called biomass. This chapter will discuss technologies for production of liquid transportation biofuels from renewable feedstocks, but hydrogen will not be included, as the production technology and infrastructure are not near term. In addition, a specific emphasis will be placed upon the research opportunities and potential for application of system biology tools to dissect and understand the biological processes central to production of these biofuels from biomass and biological materials. There are a number of technologies for production of each of these biofuels that range from fully mature processes such as grain-derived ethanol, emerging technology of ethanol form cellulose derived ethanol and immature processes such thermochemical conversion technologies and production of hydrogen all produced from renewable biological feedstocks. Conversion of biomass by various thermochemical and combustion technologies to produce thermochemical biodiesel or steam and electricity provide growing sources of bioenergy. However, these technologies are outside of the scope of this chapter, as is the use of biological processing for upgrading and conversion of fossil fuels. Therefore, this chapter will focus on the current status of production of biofuels produced from biological-derived feedstocks using biological processes. Regardless of the status of development of the biological process for production of the biofuels, each process can benefit from

  12. Bio-fuels

    International Nuclear Information System (INIS)

    2008-01-01

    This report presents an overview of the technologies which are currently used or presently developed for the production of bio-fuels in Europe and more particularly in France. After a brief history of this production since the beginning of the 20. century, the authors describe the support to agriculture and the influence of the Common Agricultural Policy, outline the influence of the present context of struggle against the greenhouse effect, and present the European legislative context. Data on the bio-fuels consumption in the European Union in 2006 are discussed. An overview of the evolution of the activity related to bio-fuels in France, indicating the locations of ethanol and bio-diesel production facilities, and the evolution of bio-fuel consumption, is given. The German situation is briefly presented. Production of ethanol by fermentation, the manufacturing of ETBE, the bio-diesel production from vegetable oils are discussed. Second generation bio-fuels are then presented (cellulose enzymatic processing), together with studies on thermochemical processes and available biomass resources

  13. Panorama 2018 - 2017 biofuels scoreboard

    International Nuclear Information System (INIS)

    Boute, Anne; Lorne, Daphne

    2018-01-01

    This note presents some 2017 statistical data about biofuels: consumption, fuel substitution rate, world ethanol and bio-diesel markets, diesel substitutes, French market, R and D investments, political measures for biofuels development

  14. Biofuels: making tough choices

    Energy Technology Data Exchange (ETDEWEB)

    Vermeulen, Sonja; Dufey, Annie; Vorley, Bill

    2008-02-15

    The jury is still out on biofuels. But one thing at least is certain: serious trade-offs are involved in the production and use of these biomass-derived alternatives to fossil fuels. This has not been lost on the European Union. The year kicked off with an announcement from the EU environment commissioner that it may be better for the EU to miss its target of reaching 10 per cent biofuel content in road fuels by 2020 than to compromise the environment and human wellbeing. The 'decision tree' outlined here can guide the interdependent processes of deliberation and analysis needed for making tough choices in national biofuels development.

  15. Integrated biofuels process synthesis

    DEFF Research Database (Denmark)

    Torres-Ortega, Carlo Edgar; Rong, Ben-Guang

    2017-01-01

    Second and third generation bioethanol and biodiesel are more environmentally friendly fuels than gasoline and petrodiesel, andmore sustainable than first generation biofuels. However, their production processes are more complex and more expensive. In this chapter, we describe a two-stage synthesis......% used for bioethanol process), and steam and electricity from combustion (54%used as electricity) in the bioethanol and biodiesel processes. In the second stage, we saved about 5% in equipment costs and 12% in utility costs for bioethanol separation. This dual synthesis methodology, consisting of a top......-level screening task followed by a down-level intensification task, proved to be an efficient methodology for integrated biofuel process synthesis. The case study illustrates and provides important insights into the optimal synthesis and intensification of biofuel production processes with the proposed synthesis...

  16. Microalgae biofuel potentials (review).

    Science.gov (United States)

    Ghasemi, Y; Rasoul-Amini, S; Naseri, A T; Montazeri-Najafabady, N; Mobasher, M A; Dabbagh, F

    2012-01-01

    With the decrease of fossil based fuels and the environmental impact of them over the planet, it seems necessary to seek the sustainable sources of clean energy. Biofuels, is becoming a worldwide leader in the development of renewable energy resources. It is worthwhile to say that algal biofuel production is thought to help stabilize the concentration of carbon dioxide in the atmosphere and decrease global warming impacts. Also, among algal fuels' attractive characteristics, algal biodiesel is non toxic, with no sulfur, highly biodegradable and relatively harmless to the environment if spilled. Algae are capable of producing in excess of 30 times more oil per acre than corn and soybean crops. Currently, algal biofuel production has not been commercialized due to high costs associated with production, harvesting and oil extraction but the technology is progressing. Extensive research was conducted to determine the utilization of microalgae as an energy source and make algae oil production commercially viable.

  17. Laccase applications in biofuels production: current status and future prospects.

    Science.gov (United States)

    Kudanga, Tukayi; Le Roes-Hill, Marilize

    2014-08-01

    The desire to reduce dependence on the ever diminishing fossil fuel reserves coupled with the impetus towards green energy has seen increased research in biofuels as alternative sources of energy. Lignocellulose materials are one of the most promising feedstocks for advanced biofuels production. However, their utilisation is dependent on the efficient hydrolysis of polysaccharides, which in part is dependent on cost-effective and benign pretreatment of biomass to remove or modify lignin and release or expose sugars to hydrolytic enzymes. Laccase is one of the enzymes that are being investigated not only for potential use as pretreatment agents in biofuel production, mainly as a delignifying enzyme, but also as a biotechnological tool for removal of inhibitors (mainly phenolic) of subsequent enzymatic processes. The current review discusses the major advances in the application of laccase as a potential pretreatment strategy, the underlying principles as well as directions for future research in the search for better enzyme-based technologies for biofuel production. Future perspectives could include synergy between enzymes that may be required for optimal results and the adoption of the biorefinery concept in line with the move towards the global implementation of the bioeconomy strategy.

  18. Biofuels, poverty, and growth

    DEFF Research Database (Denmark)

    Arndt, Channing; Benfica, Rui; Tarp, Finn

    2010-01-01

    and accrual of land rents to smallholders, compared with the more capital-intensive plantation approach. Moreover, the benefits of outgrower schemes are enhanced if they result in technology spillovers to other crops. These results should not be taken as a green light for unrestrained biofuels development...... Mozambique's annual economic growth by 0.6 percentage points and reduces the incidence of poverty by about 6 percentage points over a 12-year phase-in period. Benefits depend on production technology. An outgrower approach to producing biofuels is more pro-poor, due to the greater use of unskilled labor...

  19. The Brazilian biofuels industry

    Directory of Open Access Journals (Sweden)

    Goldemberg José

    2008-05-01

    Full Text Available Abstract Ethanol is a biofuel that is used as a replacement for approximately 3% of the fossil-based gasoline consumed in the world today. Most of this biofuel is produced from sugarcane in Brazil and corn in the United States. We present here the rationale for the ethanol program in Brazil, its present 'status' and its perspectives. The environmental benefits of the program, particularly the contribution of ethanol to reducing the emission of greenhouse gases, are discussed, as well as the limitations to its expansion.

  20. Outlook for advanced biofuels

    International Nuclear Information System (INIS)

    Hamelinck, Carlo N; Faaij, Andre P.C.

    2006-01-01

    To assess which biofuels have the better potential for the short-term or the longer term (2030), and what developments are necessary to improve the performance of biofuels, the production of four promising biofuels-methanol, ethanol, hydrogen, and synthetic diesel-is systematically analysed. This present paper summarises, normalises and compares earlier reported work. First, the key technologies for the production of these fuels, such as gasification, gas processing, synthesis, hydrolysis, and fermentation, and their improvement options are studied and modelled. Then, the production facility's technological and economic performance is analysed, applying variations in technology and scale. Finally, likely biofuels chains (including distribution to cars, and end-use) are compared on an equal economic basis, such as costs per kilometre driven. Production costs of these fuels range 16-22 Euro /GJ HHV now, down to 9-13 Euro /GJ HHV in future (2030). This performance assumes both certain technological developments as well as the availability of biomass at 3 Euro /GJ HHV . The feedstock costs strongly influence the resulting biofuel costs by 2-3 Euro /GJ fuel for each Euro /GJ HHV feedstock difference. In biomass producing regions such as Latin America or the former USSR, the four fuels could be produced at 7-11 Euro /GJ HHV compared to diesel and gasoline costs of 7 and 8 Euro /GJ (excluding distribution, excise and VAT; at crude oil prices of ∼35 Euro /bbl or 5.7 Euro /GJ). The uncertainties in the biofuels production costs of the four selected biofuels are 15-30%. When applied in cars, biofuels have driving costs in ICEVs of about 0.18-0.24 Euro /km now (fuel excise duty and VAT excluded) and may be about 0.18 in future. The cars' contribution to these costs is much larger than the fuels' contribution. Large-scale gasification, thorough gas cleaning, and micro-biological processes for hydrolysis and fermentation are key major fields for RD and D efforts, next to

  1. Improving EU biofuels policy?

    DEFF Research Database (Denmark)

    Swinbank, Alan; Daugbjerg, Carsten

    2013-01-01

    to be 'like' a compliant biofuel. A more economically rational way to reduce GHG emissions, and one that might attract greater public support, would be for the RED to reward emission reductions along the lines of the FQD. Moreover, this modification would probably make the provisions more acceptable...... in the WTO, as there would be a clearer link between policy measures and the objective of reductions in GHG emissions; and the combination of the revised RED and the FQD would lessen the commercial incentive to import biofuels with modest GHG emission savings, and thus reduce the risk of trade tension....

  2. Washington State Biofuels Industry Development

    Energy Technology Data Exchange (ETDEWEB)

    Gustafson, Richard [Univ. of Washington, Seattle, WA (United States)

    2017-04-09

    The funding from this research grant enabled us to design, renovate, and equip laboratories to support University of Washington biofuels research program. The research that is being done with the equipment from this grant will facilitate the establishment of a biofuels industry in the Pacific Northwest and enable the University of Washington to launch a substantial biofuels and bio-based product research program.

  3. Contemporary enzyme based technologies for bioremediation: A review.

    Science.gov (United States)

    Sharma, Babita; Dangi, Arun Kumar; Shukla, Pratyoosh

    2018-03-15

    The persistent disposal of xenobiotic compounds like insecticides, pesticides, fertilizers, plastics and other hydrocarbon containing substances is the major source of environmental pollution which needs to be eliminated. Many contemporary remediation methods such as physical, chemical and biological are currently being used, but they are not sufficient to clean the environment. The enzyme based bioremediation is an easy, quick, eco-friendly and socially acceptable approach used for the bioremediation of these recalcitrant xenobiotic compounds from the natural environment. Several microbial enzymes with bioremediation capability have been isolated and characterized from different natural sources, but less production of such enzymes is a limiting their further exploitation. The genetic engineering approach has the potential to get large amount of recombinant enzymes. Along with this, enzyme immobilization techniques can boost the half-life, stability and activity of enzymes at a significant level. Recently, nanozymes may offer the potential bioremediation ability towards a broad range of pollutants. In the present review, we have described a brief overview of the microbial enzymes, different enzymes techniques (genetic engineering and immobilization of enzymes) and nanozymes involved in bioremediation of toxic, carcinogenic and hazardous environmental pollutants. Copyright © 2018 Elsevier Ltd. All rights reserved.

  4. Technology Roadmaps: Biofuels for Transport

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2011-07-01

    Biofuels could provide up to 27% of total transport fuel worldwide by 2050. The use of transport fuels from biomass, when produced sustainably, can help cut petroleum use and reduce CO2 emissions in the transport sector, especially in heavy transport. Sustainable biofuel technologies, in particular advanced biofuels, will play an important role in achieving this roadmap vision. The roadmap describes the steps necessary to realise this ambitious biofuels target; identifies key actions by different stakeholders, and the role for government policy to adopt measures needed to ensure the sustainable expansion of both conventional and advanced biofuel production.

  5. The biofuels in debate

    International Nuclear Information System (INIS)

    Rigaud, Ch.

    2007-01-01

    As the development of the biofuels is increasing in the world, many voices are beginning to rise to denounce the environmental risks and the competition of the green fuels with the alimentary farming. The debate points out the problems to solve to develop a sustainable channel. (A.L.B.)

  6. Biofuel seeks endorsement

    NARCIS (Netherlands)

    Jongeneel, C.; Rentmeester, S.

    2015-01-01

    Biofuels such as ethanol from sugar cane and cellulose ‘waste’ are theoretically sustainable, as their combustion releases no more CO2 than is absorbed during production. Even so, they are also controversial, because they are believed to be grown at the expense of food crops, or because areas of

  7. Biofuel impacts on water.

    Energy Technology Data Exchange (ETDEWEB)

    Tidwell, Vincent Carroll; Malczynski, Leonard A.; Sun, Amy Cha-Tien

    2011-01-01

    Sandia National Laboratories and General Motors Global Energy Systems team conducted a joint biofuels systems analysis project from March to November 2008. The purpose of this study was to assess the feasibility, implications, limitations, and enablers of large-scale production of biofuels. 90 billion gallons of ethanol (the energy equivalent of approximately 60 billion gallons of gasoline) per year by 2030 was chosen as the book-end target to understand an aggressive deployment. Since previous studies have addressed the potential of biomass but not the supply chain rollout needed to achieve large production targets, the focus of this study was on a comprehensive systems understanding the evolution of the full supply chain and key interdependencies over time. The supply chain components examined in this study included agricultural land use changes, production of biomass feedstocks, storage and transportation of these feedstocks, construction of conversion plants, conversion of feedstocks to ethanol at these plants, transportation of ethanol and blending with gasoline, and distribution to retail outlets. To support this analysis, we developed a 'Seed to Station' system dynamics model (Biofuels Deployment Model - BDM) to explore the feasibility of meeting specified ethanol production targets. The focus of this report is water and its linkage to broad scale biofuel deployment.

  8. The rationality of biofuels

    International Nuclear Information System (INIS)

    Horta Nogueira, Luiz Augusto; Moreira, Jose Roberto; Schuchardt, Ulf; Goldemberg, Jose

    2013-01-01

    In an editorial of a recent issue of a known academic journal, Prof. Hartmut Michel affirmed that “…the production of biofuels constitutes an extremely inefficient land use… We should not grow plants for biofuel production.”, after comparing the area occupied with plants for bioenergy production with the one required for photovoltaic cells to supply the same amount of energy for transportation. This assertion is not correct for all situations and this comparison deserves a more careful analysis, evaluating the actual and prospective technological scenarios and other relevant aspects, such as capacity requirements, energy consumed during the life cycle of energy systems and the associated impacts. In this communication this comparison is revaluated, presenting a different perspective, more favorable for the bioenergy routes. - Highlights: • Energy systems and life cycle impacts are compared under equal conditions. • The comparison is done between biofuels and photovoltaic/battery in mobility uses. • Biofuels are a valuable option when produced sustainably by efficient routes

  9. Bio-fuels barometer

    International Nuclear Information System (INIS)

    Anon.

    2010-01-01

    European Union bio-fuel use for transport reached 12 million tonnes of oil equivalent (mtoe) threshold during 2009. The slowdown in the growth of European consumption deepened again. Bio-fuel used in transport only grew by 18.7% between 2008 and 2009, as against 30.3% between 2007 and 2008 and 41.8% between 2006 and 2007. The bio-fuel incorporation rate in all fuels used by transport in the E.U. is unlikely to pass 4% in 2009. We can note that: -) the proportion of bio-fuel in the German fuels market has plummeted since 2007: from 7.3% in 2007 to 5.5% in 2009; -) France stays on course with an incorporation rate of 6.25% in 2009; -) In Spain the incorporation rate reached 3.4% in 2009 while it was 1.9% in 2008. The European bio-diesel industry has had another tough year. European production only rose by 16.6% in 2009 or by about 9 million tonnes which is well below the previous year-on-year growth rate recorded (35.7%). France is leading the production of bio-ethanol fuels in Europe with an output of 1250 million liters in 2009 while the total European production reached 3700 million litters and the world production 74000 million liters. (A.C.)

  10. Making biofuels sustainable

    International Nuclear Information System (INIS)

    Gallagher, Ed

    2008-01-01

    Full text: As the twentieth century drew to a close, there was considerable support for the use of biofuels as a source of renewable energy. To many people, they offered significant savings in greenhouse gas emissions compared to fossil fuels, an opportunity for reduced dependency on oil for transport, and potential as a counter weight to increasing oil prices. They also promised an opportunity for rural economies to benefit from a new market for their products and a chance of narrowing the gap between rich and poor nations. Biofuel development was encouraged by government subsidies, and rapid growth occurred in many parts of the world. Forty per cent of Brazilian sugar cane is used for biofuel production, for example, as is almost a quarter of maize grown in the United States. Although only around 1 per cent of arable land is cultivated to grow feedstock for biofuels, there has been increasing concern over the way a largely unchecked market has developed, and about its social and environmental consequences. Recent research has confirmed that food prices have been driven significantly higher by competition for prime agricultural land and that savings in greenhouse gas emissions are much smaller - and in some cases entirely eliminated - when environmentally important land, such as rainforest, is destroyed to grow biofuels. As a result, many now believe that the economic benefits of biofuels have been obtained at too high a social and environmental price, and they question whether they can be a truly sustainable source of energy. The United Kingdom has always had sustainability at the heart of its biofuel policies and set up the Renewable Fuels Agency to ensure that this goal was met. The direct effects of biofuel production are already being assessed through five measures of environmental performance and two measures of social performance, as well as measures of the energy efficiency of the production processes used and of the greenhouse gas savings achieved

  11. Outlook for advanced biofuels

    NARCIS (Netherlands)

    Hamelinck, Carlo Noël

    2004-01-01

    Modern use of biomass can play an important role in a sustainable energy supply. Biomass abounds in most parts of the world and substantial amounts could be produced at low costs. Motor biofuels seem a sensible application of biomass: they are among the few sustainable alternatives to the

  12. The EU's Biofuel Strategy

    International Nuclear Information System (INIS)

    2006-01-01

    The EU is supporting biofuels, with the aim of reducing greenhouse-gas emission, encouraging the decarbonisation of fuels used in transportation, diversifying energy procurement, offering new earning opportunities in rural areas, and developing long-term replacements for oil. We publish lengthy excerpts from the recent Communication, COM(2006) 34def. which describes the strategy adopted by the Commission [it

  13. Smart choices for biofuels

    Science.gov (United States)

    2009-01-01

    Much of the strong support for biofuels in the United States is premised on the national security advantages of reducing dependence on imported oil. In late 2007, these expected payoffs played a major role in driving an extension and expansion of the...

  14. Cell manipulation in microfluidics

    International Nuclear Information System (INIS)

    Yun, Hoyoung; Kim, Kisoo; Lee, Won Gu

    2013-01-01

    Recent advances in the lab-on-a-chip field in association with nano/microfluidics have been made for new applications and functionalities to the fields of molecular biology, genetic analysis and proteomics, enabling the expansion of the cell biology field. Specifically, microfluidics has provided promising tools for enhancing cell biological research, since it has the ability to precisely control the cellular environment, to easily mimic heterogeneous cellular environment by multiplexing, and to analyze sub-cellular information by high-contents screening assays at the single-cell level. Various cell manipulation techniques in microfluidics have been developed in accordance with specific objectives and applications. In this review, we examine the latest achievements of cell manipulation techniques in microfluidics by categorizing externally applied forces for manipulation: (i) optical, (ii) magnetic, (iii) electrical, (iv) mechanical and (v) other manipulations. We furthermore focus on history where the manipulation techniques originate and also discuss future perspectives with key examples where available. (topical review)

  15. Microfluidic chemical reaction circuits

    Science.gov (United States)

    Lee, Chung-cheng [Irvine, CA; Sui, Guodong [Los Angeles, CA; Elizarov, Arkadij [Valley Village, CA; Kolb, Hartmuth C [Playa del Rey, CA; Huang, Jiang [San Jose, CA; Heath, James R [South Pasadena, CA; Phelps, Michael E [Los Angeles, CA; Quake, Stephen R [Stanford, CA; Tseng, Hsian-rong [Los Angeles, CA; Wyatt, Paul [Tipperary, IE; Daridon, Antoine [Mont-Sur-Rolle, CH

    2012-06-26

    New microfluidic devices, useful for carrying out chemical reactions, are provided. The devices are adapted for on-chip solvent exchange, chemical processes requiring multiple chemical reactions, and rapid concentration of reagents.

  16. Microalgae: biofuel production

    Directory of Open Access Journals (Sweden)

    Babita Kumari

    2013-04-01

    Full Text Available In the present day, microalgae feedstocks are gaining interest in energy scenario due to their fast growth potential coupled with relatively high lipid, carbohydrate and nutrients contents. All of these properties render them an excellent source for biofuels such as biodiesel, bioethanol and biomethane; as well as a number of other valuable pharmaceutical and nutraceutical products. The present review is a critical appraisal of the commercialization potential of microalgae biofuels. The available literature on various aspects of microalgae for e.g. its cultivation, life cycle assessment, and conceptualization of an algal biorefinery, has been done. The evaluation of available information suggests the operational and maintenance cost along with maximization of oil-rich microalgae production is the key factor for successful commercialization of microalgae-based fuels.

  17. The bio-fuels

    International Nuclear Information System (INIS)

    Levy, R.H.

    1993-02-01

    In France, using fallow soils for energy production may be a solution to agriculture problems. Technical and economical studies of biofuels (ethanol, methanol, ethyl tributyl ether, methyl tributyl ether and methyl ester) are presented with costs of production from the raw material to the end product, characteristics of the end product, engine consumption for pure or mixed fuels, and environmental impacts. For the author, the mixed ethanol process shows no advantages in term of energy dependency (ETBE, MTBE and colza ester give better results), ethanol production uses 90% and colza ester production 53% of the calorific power of the produced biofuels. Commercial balance: damaged, fiscal receipts: reduced, new jobs creation: inferior to 10.000 and the majority outside of the agriculture sphere, environmental impacts: slight diminution of greenhouse gases, but growth of soil and water pollution, all these points are developed by the author. Observations of some contradictors are also given. (A.B.). refs. figs., tabs

  18. Biofuels made easy

    International Nuclear Information System (INIS)

    Hamilton, C.

    2004-01-01

    Much has been said and written in Australia since the Federal Government introduced its Clean Fuels Policy in September 2001. Various biofuel projects are now being considered in different states of Australia for the manufacture of bioethanol and biodiesel from renewable resources. However, the economic viability required to establish an Australian liquid biofuels industry is predicated on supportive government legislation and an encouraging fuel excise regime. On the other hand, the benefits of such an industry are also in debate. In an attempt to clarify some of the concerns being raised, this paper endeavours to provide an overview of the current use of bioethanol and biodiesel around the world, to summarise the process technologies involved, to review the benefits and non-benefits of renewable fuels to the transport industry and to address the issues for such an industry here in Australia

  19. Microfluidics without channels: highly-flexible synthesis on a digital-microfluidic chip for production of diverse PET tracers

    Energy Technology Data Exchange (ETDEWEB)

    Van Dam, Robert Michael [Univ. of California, Los Angeles, CA (United States)

    2010-09-01

    Positron emission tomography (PET) imaging is used for fundamental studies of living biological organisms and microbial ecosystems in applications ranging from biofuel production to environmental remediation to the study, diagnosis, and treatment monitoring of human disease. Routine access to PET imaging, to monitor biochemical reactions in living organisms in real time, could accelerate a broad range of research programs of interest to DOE. Using PET requires access to short-lived radioactive-labeled compounds that specifically probe the desired living processes. The overall aims of this project were to develop a miniature liquid-handling technology platform (called “microfluidics”) that increases the availability of diverse PET probes by reducing the cost and complexity of their production. Based on preliminary experiments showing that microfluidic chips can synthesis such compounds, we aimed to advance this technology to improve its robustness, increase its flexibility for a broad range of probes, and increase its user-friendliness. Through the research activities of this project, numerous advances were made; Tools were developed to enable the visualization of radioactive materials within microfluidic chips; Fundamental advances were made in the microfluidic chip architecture and fabrication process to increase its robustness and reliability; The microfluidic chip technology was shown to produce useful quantities of an example PET probes, and methods to further increase the output were successfully pursued; A “universal” chip was developed that could produce multiple types of PET probes, enabling the possibility of “on demand” synthesis of different probes; and Operation of the chip was automated to ensure minimal radiation exposure to the operator Based on the demonstrations of promising technical feasibility and performance, the microfluidic chip technology is currently being commercialized. It is anticipated that costs of microfluidic chips can be

  20. Biofuel market and carbon modeling to analyse French biofuel policy

    International Nuclear Information System (INIS)

    Bernard, F.; Prieur, A.

    2007-01-01

    In order to comply with European Union objectives, France has set up an ambitious biofuel plan. This plan is evaluated on the basis of two criteria: tax exemption on fossil fuels and greenhouse gases (GHG) emission savings. An economic marginal analysis and a life cycle assessment (LCA) are provided using a coupling procedure between a partial agro-industrial equilibrium model and an oil refining optimization model. Thus, we determine the minimum tax exemption needed to place on the market a targeted quantity of biofuel by deducting the biofuel long-run marginal revenue of refiners from the agro-industrial marginal cost of biofuel production. With a clear view of the refiner's economic choices, total pollutant emissions along the biofuel production chains are quantified and used to feed an LCA. The French biofuel plan is evaluated for 2008, 2010 and 2012 using prospective scenarios. Results suggest that biofuel competitiveness depends on crude oil prices and demand for petroleum products and consequently these parameters should be taken into account by authorities to modulate biofuel tax exemption. LCA results show that biofuel production and use, from 'seed to wheel', would facilitate the French Government's compliance with its 'Plan Climat' objectives by reducing up to 5% GHG emissions in the French road transport sector by 2010

  1. Biofuel market and carbon modeling to evaluate French biofuel policy

    International Nuclear Information System (INIS)

    Bernard, F.; Prieur, A.

    2006-10-01

    In order to comply with European objectives, France has set up an ambitious biofuel plan. This plan is evaluated considering two criteria: tax exemption need and GHG emission savings. An economic marginal analysis and a life cycle assessment (LCA) are provided using a coupling procedure between a partial agro-industrial equilibrium model and a refining optimization model. Thus, we are able to determine the minimum tax exemption needed to place on the market a targeted quantity of biofuel by deducing the agro-industrial marginal cost of biofuel production to the biofuel refining long-run marginal revenue. In parallel, a biofuels LCA is carried out using model outputs. Such a method avoid common allocation problems between joint products. The French biofuel plan is evaluated for 2008, 2010 and 2012 using prospective scenarios. Results suggest that biofuel competitiveness depends on crude oil prices and petroleum products demands. Consequently, biofuel tax exemption does not always appear to be necessary. LCA results show that biofuels production and use, from 'seed to wheel', would facilitate the French Government's to compliance with its 'Plan Climat' objectives by reducing up to 5% GHG emissions in the French road transport sector by 2010. (authors)

  2. Bio-fuels - biohazard

    International Nuclear Information System (INIS)

    Slovak, K.

    2008-01-01

    Politicians have a clear explanation for growing commodity prices. It is all the fault of speculators. It is easy to point the finger at an imaginary enemy. It is more difficult and from the point of view of a political career suicidal to admit one's mistakes. And there are reasons for remorse. According to studies prepared by the OECD and the World Bank bio-fuels are to be blame for high food prices. The bio-fuel boom that increases the demand for agro-commodities has been created by politicians offering generous subsidies. And so farming products do not end up on the table, but in the fuel tanks of cars in the form of additives. And their only efficiency is that they make food more expensive. The first relevant indication that environmentalist tendencies in global politics have resulted in shortages and food price increases can be found in a confidential report prepared by the World Bank. Parts of the report were leaked to the media last month. According to this information growing bio-fuel production has resulted in a food price increase by 75%. The theory that this development was caused by speculators and Chinese and Indian demand received a serious blow. And the OECD report definitely contradicted the excuse used by the politicians. According to the report one of the main reasons for growing food prices are generously subsidized bio-fuels. Their share of the increase of demand for agro-commodities in 2005 -2007 was 60% according to the study. (author)

  3. Biofuels barometer: Crops pending

    International Nuclear Information System (INIS)

    Anon.

    2002-01-01

    The actors and production capacities have changed only little in the biofuel sector from year to another. Nevertheless, it is interesting to take stock of the development of this sector at the end of 2002, so as to update the more complete barometer published in issue 144 of Systemes Solaires. Indeed, European ethanol production grew by 13% and that of bio-diesel by more than 20% in 2001. (authors)

  4. Biofuels and WTO Disciplines

    OpenAIRE

    Brühwiler, Claudia Franziska; Hauser, Heinz

    2008-01-01

    Given the sharp rise in crude oil prices and growing awareness of climate change, the potential of biofuels, particularly of bioethanol, has become an ubiquitous topic of public debate and has induced ambitious policy initiatives. The latter are mostly paired with protectionist measures as the examples of the European Union and the United States show, where domestic producers of energy crops are put at an advantage thanks to subsidisation, direct payments and/or favourable tax schemes.Moreove...

  5. Biofuels - the UFIP position

    International Nuclear Information System (INIS)

    2004-01-01

    Since 2003 a directive promote the biofuels use. The industry is then using them in ETBE (Ethyl Tertio Butyl Ether) fuels and in diesel oil of vegetal oils esters EMHV. Meanwhile some of them present technical difficulties and must free themselves from fiscal incentives which make them competitive. For these reasons, the UFIP (french union of petroleum industries) do not agree their obligatory incorporation. (A.L.B.)

  6. Hawaii Algal Biofuel

    Science.gov (United States)

    2013-03-01

    Spirulina Algea, Swine Manure , and Digested Anaerobic Sludge." Bioresource Technology 102: 8295- 8303. Viets, John W., Narasimhan Sundaram, Bal K. Kaul, and...biofuel source. Dr. Zimmerman noted that since algae decompose easily in landfills, the nutrients produced by anaerobic digestion of biomass can be...resource requirements would be pivotal to the offices of the U.S. Navy Resource entities such as OPNAV. In order for decision makers to digest the

  7. Potentials of biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Munack, A.; Schroder, O. [Johann Heinrich von Thunen Inst., Braunschweig (Germany); Krahl, J. [Coburg Univ. of Applied Sciences, Coburg (Germany); Bunger, J. [Inst. for Prevention and Occupational Medicine of the German Social Accident Insurance, Ruhr-Univ. Inst., Bochum (Germany)

    2010-07-01

    This paper discussed the potential of biofuels with particular reference to the situation in Germany and Europe. Emphasis was on technical potential, such as biofuel production, utilization and environmental aspects. The Institute of Agricultural Technology and Biosystems Engineering ran vTI emission tests on diesel engines to evaluate the environmental impacts of biofuels. This testing facility is able to drive heavy-duty diesel engines in both stationary and dynamic test cycles, such as the European ESC and ETC. Additional analyses were conducted to determine the fine and ultra-fine particles, polycyclic aromatic hydrocarbons (PAH), aldehydes, ketones, and the usual regulated exhaust gas compounds. Ames tests were conducted to assess the mutagenic potential of tailpipe emissions. Previous study results showed that neat vegetable oils can render the exhaust high in mutagenic potency. Some of the non-regulated exhaust gas compounds were found to vary nonlinearly with the blend composition. B20 was found to have high mutagenic potential and was subject to sedimentation.

  8. Biofuels from microbes

    Energy Technology Data Exchange (ETDEWEB)

    Antoni, D. [Technische Univ. Muenchen, Freising-Weihenstephan (Germany). Inst. of Resource and Energy Technology; Zverlov, V.V.; Schwarz, W.H. [Technische Univ. Muenchen, Freising-Weihenstephan (Germany). Dept. of Microbiology

    2007-11-15

    Today, biomass covers about 10% of the world's primary energy demand. Against a backdrop of rising crude oil prices, depletion of resources, political instability in producing countries and environmental challenges, besides efficiency and intelligent use, only biomass has the potential to replace the supply of an energy hungry civilisation. Plant biomass is an abundant and renewable source of energy-rich carbohydrates which can be efficiently converted by microbes into biofuels, of which, only bioethanol is produced on an industrial scale today. Biomethane is produced on a large scale, but is not yet utilised for transportation. Biobutanol is on the agenda of several companies and may be used in the near future as a supplement for gasoline, diesel and kerosene, as well as contributing to the partially biological production of butyl-t-butylether, BTBE as does bioethanol today with ETBE. Biohydrogen, biomethanol and microbially made biodiesel still require further development. This paper reviews microbially made biofuels which have potential to replace our present day fuels, either alone, by blending, or by chemical conversion. It also summarises the history of biofuels and provides insight into the actual production in various countries, reviewing their policies and adaptivity to the energy challenges of foreseeable future. (orig.)

  9. Benchmarking biofuels; Biobrandstoffen benchmarken

    Energy Technology Data Exchange (ETDEWEB)

    Croezen, H.; Kampman, B.; Bergsma, G.

    2012-03-15

    A sustainability benchmark for transport biofuels has been developed and used to evaluate the various biofuels currently on the market. For comparison, electric vehicles, hydrogen vehicles and petrol/diesel vehicles were also included. A range of studies as well as growing insight are making it ever clearer that biomass-based transport fuels may have just as big a carbon footprint as fossil fuels like petrol or diesel, or even bigger. At the request of Greenpeace Netherlands, CE Delft has brought together current understanding on the sustainability of fossil fuels, biofuels and electric vehicles, with particular focus on the performance of the respective energy carriers on three sustainability criteria, with the first weighing the heaviest: (1) Greenhouse gas emissions; (2) Land use; and (3) Nutrient consumption [Dutch] Greenpeace Nederland heeft CE Delft gevraagd een duurzaamheidsmeetlat voor biobrandstoffen voor transport te ontwerpen en hierop de verschillende biobrandstoffen te scoren. Voor een vergelijk zijn ook elektrisch rijden, rijden op waterstof en rijden op benzine of diesel opgenomen. Door onderzoek en voortschrijdend inzicht blijkt steeds vaker dat transportbrandstoffen op basis van biomassa soms net zoveel of zelfs meer broeikasgassen veroorzaken dan fossiele brandstoffen als benzine en diesel. CE Delft heeft voor Greenpeace Nederland op een rijtje gezet wat de huidige inzichten zijn over de duurzaamheid van fossiele brandstoffen, biobrandstoffen en elektrisch rijden. Daarbij is gekeken naar de effecten van de brandstoffen op drie duurzaamheidscriteria, waarbij broeikasgasemissies het zwaarst wegen: (1) Broeikasgasemissies; (2) Landgebruik; en (3) Nutriëntengebruik.

  10. European biofuel policies in retrospect

    International Nuclear Information System (INIS)

    Van Thuijl, E.; Deurwaarder, E.P.

    2006-05-01

    Despite the benefits of the production and use of biofuels in the fields of agriculture, security of energy supply and the environment, in India and surrounding countries, the barriers to the use of biofuels are still substantial. The project ProBios (Promotion of Biofuels for Sustainable Development in South and South East Asia) aims at promoting biofuels in the view of sustainable development in the Southern and South eastern Asian countries. The first stage of this project concerns a study, which will provide a thorough review of the complicated and sector-overarching issue of biofuels in India and surrounding countries. This report describes past experiences with the policy context for a selection of EU countries, with the purpose of identifying conclusions from the European experience that may be valuable for Indian and South East Asian policy makers and other biofuels stakeholders

  11. Sustainability development: Biofuels in agriculture

    OpenAIRE

    Cheteni, Priviledge

    2017-01-01

    Biofuels are socially and politically accepted as a form of sustainable energy in numerous countries. However, cases of environmental degradation and land grabs have highlighted the negative effects to their adoption. Smallholder farmers are vital in the development of a biofuel industry. The study sort to assess the implications in the adoption of biofuel crops by smallholder farmers. A semi-structured questionnaire was administered to 129 smallholder farmers who were sampled from the Easter...

  12. Biofuels: from microbes to molecules

    National Research Council Canada - National Science Library

    Lu, Xuefeng

    2014-01-01

    .... The production of different biofuel molecules including hydrogen, methane, ethanol, butanol, higher chain alcohols, isoprenoids and fatty acid derivatives, from genetically engineered microbes...

  13. Biofuel technology handbook. 2. ed.

    Energy Technology Data Exchange (ETDEWEB)

    Rutz, Dominik; Janssen, Rainer

    2008-01-15

    This comprehensive handbook was created in order to promote the production and use of biofuels and to inform politicians, decision makers, biofuel traders and all other relevant stakeholders about the state-of-the-art of biofuels and relevant technologies. The large variety of feedstock types and different conversion technologies are described. Explanations about the most promising bio fuels provide a basis to discuss about the manifold issues of biofuels. The impartial information in this handbook further contributes to diminish existing barriers for the broad use of biofuels. Emphasis of this handbook is on first generation biofuels: bio ethanol, Biodiesel, pure plant oil, and bio methane. It also includes second generation biofuels such as BTL-fuels and bio ethanol from lingo-cellulose as well as bio hydrogen. The whole life cycle of bio fuels is assessed under technical, economical, ecological, and social aspect. Characteristics and applications of bio fuels for transport purposes are demonstrated and evaluated. This is completed by an assessment about the most recent studies on biofuel energy balances. This handbook describes the current discussion about green house gas (GHG) balances and sustainability aspects. GHG calculation methods are presented and potential impacts of biofuel production characterized: deforestation of rainforests and wetlands, loss of biodiversity, water pollution, human health, child labour, and labour conditions.

  14. Microfluidic Flame Barrier

    Science.gov (United States)

    Mungas, Gregory S. (Inventor); Fisher, David J. (Inventor); Mungas, Christopher (Inventor)

    2013-01-01

    Propellants flow through specialized mechanical hardware that is designed for effective and safe ignition and sustained combustion of the propellants. By integrating a micro-fluidic porous media element between a propellant feed source and the combustion chamber, an effective and reliable propellant injector head may be implemented that is capable of withstanding transient combustion and detonation waves that commonly occur during an ignition event. The micro-fluidic porous media element is of specified porosity or porosity gradient selected to be appropriate for a given propellant. Additionally the propellant injector head design integrates a spark ignition mechanism that withstands extremely hot running conditions without noticeable spark mechanism degradation.

  15. Biofuels versus food production: Does biofuels production increase food prices?

    International Nuclear Information System (INIS)

    Ajanovic, Amela

    2011-01-01

    Rapidly growing fossil energy consumption in the transport sector in the last two centuries caused problems such as increasing greenhouse gas emissions, growing energy dependency and supply insecurity. One approach to solve these problems could be to increase the use of biofuels. Preferred feedstocks for current 1st generation biofuels production are corn, wheat, sugarcane, soybean, rapeseed and sunflowers. The major problem is that these feedstocks are also used for food and feed production. The core objective of this paper is to investigate whether the recent increase of biofuels production had a significant impact on the development of agricultural commodity (feedstock) prices. The most important impact factors like biofuels production, land use, yields, feedstock and crude oil prices are analysed. The major conclusions of this analysis are: In recent years the share of bioenergy-based fuels has increased moderately, but continuously, and so did feedstock production, as well as yields. So far, no significant impact of biofuels production on feedstock prices can be observed. Hence, a co-existence of biofuel and food production seems possible especially for 2nd generation biofuels. However, sustainability criteria should be seriously considered. But even if all crops, forests and grasslands currently not used were used for biofuels production it would be impossible to substitute all fossil fuels used today in transport.

  16. The price for biofuels sustainability

    International Nuclear Information System (INIS)

    Pacini, Henrique; Assunção, Lucas; Dam, Jinke van; Toneto, Rudinei

    2013-01-01

    The production and usage of biofuels has increased worldwide, seeking goals of energy security, low-carbon energy and rural development. As biofuels trade increased, the European Union introduced sustainability regulations in an attempt to reduce the risks associated with biofuels. Producers were then confronted with costs of sustainability certification, in order to access the EU market. Hopes were that sustainably-produced biofuels would be rewarded with higher prices in the EU. Based on a review of recent literature, interviews with traders and price data from Platts, this paper explores whether sustainability premiums emerged and if so, did they represent an attracting feature in the market for sustainable biofuels. This article finds that premiums for ethanol and biodiesel evolved differently between 2011 and 2012, but have been in general very small or inexistent, with certified fuels becoming the new norm in the market. For different reasons, there has been an apparent convergence between biofuel policies in the EU and the US. As market operators perceive a long-term trend for full certification in the biofuels market, producers in developing countries are likely to face additional challenges in terms of finance and capacity to cope with the sustainability requirements. - Highlights: • EU biofuel sustainability rules were once thought to reward compliant producers with price-premiums. • Premiums for certified biofuels, however, have been small for biodiesel and almost non-existent for ethanol. • As sustainable biofuels became the new norm, premiums disappeared almost completely in 2012. • Early stages of supply chains concentrate the highest compliance costs, affecting specially developing country producers. • Producers are now in a market where sustainable biofuels have become the new norm

  17. Biofuels in Central America

    International Nuclear Information System (INIS)

    Sanders, E.

    2007-08-01

    This report presents the results of an analysis of the biofuel markets in El Salvador, Panama, Costa Rica and Honduras. The aim of this report is to provide insight in the current situation and the expected developments in these markets and thus to provide investors with an image of the opportunities that could be present in this sector. An attempt has been made to provide a clear overview of this sector in the countries concerned. Due to a lack of data this has not been fully accomplished in some cases. [mk] [nl

  18. Biofuels: Project summaries

    Energy Technology Data Exchange (ETDEWEB)

    1994-07-01

    The US DOE, through the Biofuels Systems Division (BSD) is addressing the issues surrounding US vulnerability to petroleum supply. The BSD goal is to develop technologies that are competitive with fossil fuels, in both cost and environmental performance, by the end of the decade. This document contains summaries of ongoing research sponsored by the DOE BSD. A summary sheet is presented for each project funded or in existence during FY 1993. Each summary sheet contains and account of project funding, objectives, accomplishments and current status, and significant publications.

  19. Byproducts for biofuels

    International Nuclear Information System (INIS)

    Bondt, N.; Meeusen, M.J.G.

    2008-02-01

    This report examines the market for residues from the Dutch food and beverage industry, and the appeal of these residues for the production of bio-ethanol and biodiesel. The firstgeneration technology is readily suited to the conversion of no more than 29% of the 7.5 million tonnes of residues into biofuels. Moreover, when non-technological criteria are also taken into account virtually none of the residues are of interest for conversion into bioethanol, although vegetable and animal fats can be used to produce biodiesel. The economic consequences for sectors such as the animal-feed sector are limited [nl

  20. Investigating biofuels through network analysis

    International Nuclear Information System (INIS)

    Curci, Ylenia; Mongeau Ospina, Christian A.

    2016-01-01

    Biofuel policies are motivated by a plethora of political concerns related to energy security, environmental damages, and support of the agricultural sector. In response to this, much scientific work has chiefly focussed on analysing the biofuel domain and on giving policy advice and recommendations. Although innovation has been acknowledged as one of the key factors in sustainable and cost-effective biofuel development, there is an urgent need to investigate technological trajectories in the biofuel sector by starting from consistent data and appropriate methodological tools. To do so, this work proposes a procedure to select patent data unequivocally related to the investigated sector, it uses co-occurrence of technological terms to compute patent similarity and highlights content and interdependencies of biofuels technological trajectories by revealing hidden topics from unstructured patent text fields. The analysis suggests that there is a breaking trend towards modern generation biofuels and that innovators seem to focus increasingly on the ability of alternative energy sources to adapt to the transport/industrial sector. - Highlights: • Innovative effort is devoted to biofuels additives and modern biofuels technologies. • A breaking trend can be observed from the second half of the last decade. • A patent network is identified via text mining techniques that extract latent topics.

  1. Transporter-mediated biofuel secretion.

    Science.gov (United States)

    Doshi, Rupak; Nguyen, Tuan; Chang, Geoffrey

    2013-05-07

    Engineering microorganisms to produce biofuels is currently among the most promising strategies in renewable energy. However, harvesting these organisms for extracting biofuels is energy- and cost-intensive, limiting the commercial feasibility of large-scale production. Here, we demonstrate the use of a class of transport proteins of pharmacological interest to circumvent the need to harvest biomass during biofuel production. We show that membrane-embedded transporters, better known to efflux lipids and drugs, can be used to mediate the secretion of intracellularly synthesized model isoprenoid biofuel compounds to the extracellular milieu. Transporter-mediated biofuel secretion sustainably maintained an approximate three- to fivefold boost in biofuel production in our Escherichia coli test system. Because the transporters used in this study belong to the ubiquitous ATP-binding cassette protein family, we propose their use as "plug-and-play" biofuel-secreting systems in a variety of bacteria, cyanobacteria, diatoms, yeast, and algae used for biofuel production. This investigation showcases the potential of expressing desired membrane transport proteins in cell factories to achieve the export or import of substances of economic, environmental, or therapeutic importance.

  2. Numerical Optimization in Microfluidics

    DEFF Research Database (Denmark)

    Jensen, Kristian Ejlebjærg

    2017-01-01

    Numerical modelling can illuminate the working mechanism and limitations of microfluidic devices. Such insights are useful in their own right, but one can take advantage of numerical modelling in a systematic way using numerical optimization. In this chapter we will discuss when and how numerical...... optimization is best used....

  3. Enzyme detection by microfluidics

    DEFF Research Database (Denmark)

    2013-01-01

    Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted...... by that enzyme...

  4. Microfluidics for medical applications

    NARCIS (Netherlands)

    van den Berg, Albert; van den Berg, A.; Segerink, L.I.; Segerink, Loes Irene; Unknown, [Unknown

    2015-01-01

    Lab-on-a-chip devices for point of care diagnostics have been present in clinics for several years now. Alongside their continual development, research is underway to bring the organs and tissue on-a-chip to the patient, amongst other medical applications of microfluidics. This book provides the

  5. Chemistry in Microfluidic Channels

    Science.gov (United States)

    Chia, Matthew C.; Sweeney, Christina M.; Odom, Teri W.

    2011-01-01

    General chemistry introduces principles such as acid-base chemistry, mixing, and precipitation that are usually demonstrated in bulk solutions. In this laboratory experiment, we describe how chemical reactions can be performed in a microfluidic channel to show advanced concepts such as laminar fluid flow and controlled precipitation. Three sets of…

  6. Microfluidic isotachophoresis: A review

    Czech Academy of Sciences Publication Activity Database

    Smejkal, P.; Bottenus, D.; Breadmore, M. C.; Guijt, R. M.; Ivory, C. F.; Foret, František; Macka, M.

    2013-01-01

    Roč. 34, č. 11 (2013), s. 1493-1509 ISSN 0173-0835 R&D Projects: GA ČR(CZ) GAP301/11/2055 Institutional support: RVO:68081715 Keywords : chip * isotachophoresis * microfluidics * miniaturization Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 3.161, year: 2013

  7. Biofuels. An overview. Final Report

    International Nuclear Information System (INIS)

    De Castro, J.F.M.

    2007-05-01

    The overall objective of this desk study is to get an overview of the most relevant liquid biofuels especially in the African context, and more specifically in the Netherlands' relevant partner countries. The study will focus on biofuels for transport, but will also consider biofuels for cooking and power generation. Biogas as the result of anaerobic fermentation which can be used for cooking, lighting and electricity generation will not be considered in this study. Liquid biofuels are usually divided into alcohols that are used to substitute for gasoline and oils that are used to substitute for diesel and are often called Biodiesel, and this division will be followed in this study. In chapter 2 we will analyse several aspects of the use of alcohols particularly ethanol, in chapter 3 the same analysis will be done for oils, using as example the very promising Jatropha oil. In chapter we will analyse socio-economic issues of the use of these biofuels

  8. National Algal Biofuels Technology Roadmap

    Energy Technology Data Exchange (ETDEWEB)

    Ferrell, John [Dept. of Energy (DOE), Washington DC (United States); Sarisky-Reed, Valerie [Dept. of Energy (DOE), Washington DC (United States)

    2010-05-01

    The framework for National Algal Biofuels Technology Roadmap was constructed at the Algal Biofuels Technology Roadmap Workshop, held December 9-10, 2008, at the University of Maryland-College Park. The Workshop was organized by the Biomass Program to discuss and identify the critical challenges currently hindering the development of a domestic, commercial-scale algal biofuels industry. This Roadmap presents information from a scientific, economic, and policy perspectives that can support and guide RD&D investment in algal biofuels. While addressing the potential economic and environmental benefits of using algal biomass for the production of liquid transportation fuels, the Roadmap describes the current status of algae RD&D. In doing so, it lays the groundwork for identifying challenges that likely need to be overcome for algal biomass to be used in the production of economically viable biofuels.

  9. Biofuels and environment

    International Nuclear Information System (INIS)

    Wihersaari, M.

    1996-01-01

    The purpose of this work was to produce more information on the environmental impacts of biomass production and use. Energy consumption and environmental impacts of different biomass and fossil fuel production techniques combined with transportation and end use figures are needed for comparing different fuel alternatives to reach a maximum environmental benefits from the total energy system. The energy demand of different biomass production chains was calculated and compared. Special attention was paid to new production techniques, developed in the ongoing Finnish BIOENERGY research programme. The energy consumption and the emissions from biomass production were compared with the corresponding parametres for fossil fuels used in Finland. The use of biomass for energy purposes provides environmental benefits compared to fossile fuels. The most notable ones are very small or none net emissions of greenhouse gases and SO 2 when burning biomass. NO x emissions from the production and transportation chain form a notable part of the total NO x emissons of the bioenergy production and utilization chain, especially for large biomass plants, and therefore attention should be paid to the possibilities to lower these emissions. Biomass fuel production is not free from fossil fuels. About 2-6 per cent of the produced energy is used in the production chain. The amount of used energy rises much higher, if the biofuel is processed to be an alternative for e.g. fossil diesel fuels. The energy demand in the fossil fuel production chain is though greater than in the production chain of basic biofuels. (52 refs.)

  10. Biofuels and algae

    International Nuclear Information System (INIS)

    Anon.

    2011-01-01

    Bio-fuels based on micro-algae are promising, their licensing for being used in plane fuels in a mix containing 50% of fossil kerosene is expected in the coming months. In United-States research on bio-fuels has been made more important since 2006 when 2 policies were launched: 'Advanced energy initiative' and 'Twenty-in-ten', the latter aiming to develop alternative fuels. In Europe less investment has been made concerning micro-algae fuels but research programs were launched in Spain, United-Kingdom and France. In France 3 important projects were launched: SHAMASH (2006-2010) whose aim is to produce lipidic fuels from micro-algae, ALGOHUB (2008-2013) whose aim is to use micro-algae as a raw material for humane and animal food, medicine and cosmetics, SYMBIOSE (2009-2011) whose aim is the optimization of the production of methane through the anaerobic digestion of micro-algae, SALINALGUE (2010-2016) whose aim is to grow micro-algae for the production of bio-energies and bio-products. (A.C.)

  11. Biofuels: 1995 project summaries

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-01-01

    Domestic transportation fuels are derived primarily from petroleum and account for about two-thirds of the petroleum consumption in the United States. In 1994, more than 40% of our petroleum was imported. That percentage is likely to increase, as the Middle East has about 75% of the world`s oil reserves, but the United States has only about 5%. Because we rely so heavily on oil (and because we currently have no suitable substitutes for petroleum-based transportation fuels), we are strategically and economically vulnerable to disruptions in the fuel supply. Additionally, we must consider the effects of petroleum use on the environment. The Biofuels Systems Division (BSD) is part of the U.S. Department of Energy (DOE) Office of Energy Efficiency and Renewable Energy (EE). The day-to-day research activities, which address these issues, are managed by the National Renewable Energy Laboratory in Golden, Colorado, and Oak Ridge National Laboratory in Oak Ridge, Tennessee. BSD focuses its research on biofuels-liquid and gaseous fuels made from renewable domestic crops-and aggressively pursues new methods for domestically producing, recovering, and converting the feedstocks to produce the fuels economically. The biomass resources include forage grasses, oil seeds, short-rotation woody crops, agricultural and forestry residues, algae, and certain industrial and municipal waste streams. The resulting fuels include ethanol, methanol, biodiesel, and ethers.

  12. Biofuel from "humified" biomass

    Science.gov (United States)

    Kpogbemabou, D.; Lemée, L.; Amblès, A.

    2009-04-01

    In France, 26% of the emissions of greenhouse effect gas originate from transportation which depends for 87% on fossil fuels. Nevertheless biofuels can contribute to the fight against climate change while reducing energetic dependence. Indeed biomass potentially represents in France 30 Mtoe a year that is to say 15% national consumption. But 80% of these resources are made of lignocellulosic materials which are hardly exploitable. First-generation biofuels are made from sugar, starch, vegetable oil, or animal fats. Due to their competition with human food chain, first-generation biofuels could lead to food shortages and price rises. At the contrary second-generation biofuel production can use a variety of non food crops while using the lignocellulosic part of biomass [1]. Gasification, fermentation and direct pyrolysis are the most used processes. However weak yields and high hydrogen need are limiting factors. In France, the National Program for Research on Biofuels (PNRB) aims to increase mobilizable biomass resource and to develop lignocellulosic biomass conversion. In this context, the LIGNOCARB project studies the liquefaction of biodegraded biomass in order to lower hydrogen consumption. Our aim was to develop and optimize the biodegradation of the biomass. Once the reactor was achieved, the influence of different parameters (starting material, aeration, moisture content) on the biotransformation process was studied. The monitored parameters were temperature, pH and carbon /nitrogen ratio. Chemical (IHSS protocol) and biochemical (van Soest) fractionations were used to follow the maturity ("humic acid"/"fulvic acid" ratio) and the biological stability (soluble, hemicelluloses, celluloses, lignin) of the organic matter (OM). In example, the increase in lignin can be related to the stabilization since the OM becomes refractory to biodegradation whereas the increase in the AH/AF ratio traduces "humification". However, contrarily to the composting process, we do

  13. PREFACE: Nano- and microfluidics Nano- and microfluidics

    Science.gov (United States)

    Jacobs, Karin

    2011-05-01

    The field of nano- and microfluidics emerged at the end of the 1990s parallel to the demand for smaller and smaller containers and channels for chemical, biochemical and medical applications such as blood and DNS analysis [1], gene sequencing or proteomics [2, 3]. Since then, new journals and conferences have been launched and meanwhile, about two decades later, a variety of microfluidic applications are on the market. Briefly, 'the small flow becomes mainstream' [4]. Nevertheless, research in nano- and microfluidics is more than downsizing the spatial dimensions. For liquids on the nanoscale, surface and interface phenomena grow in importance and may even dominate the behavior in some systems. The studies collected in this special issue all concentrate on these type of systems and were part ot the priority programme SPP1164 'Nano- and Microfluidics' of the German Science Foundation (Deutsche Forschungsgemeinschaft, DFG). The priority programme was initiated in 2002 by Hendrik Kuhlmann and myself and was launched in 2004. Friction between a moving liquid and a solid wall may, for instance, play an important role so that the usual assumption of a no-slip boundary condition is no longer valid. Likewise, the dynamic deformations of soft objects like polymers, vesicles or capsules in flow arise from the subtle interplay between the (visco-)elasticity of the object and the viscous stresses in the surrounding fluid and, potentially, the presence of structures confining the flow like channels. Consequently, new theories were developed ( see articles in this issue by Münch and Wagner, Falk and Mecke, Bonthuis et al, Finken et al, Almenar and Rauscher, Straube) and experiments were set up to unambiguously demonstrate deviations from bulk, or 'macro', behavior (see articles in this issue by Wolff et al, Vinogradova and Belyaev, Hahn et al, Seemann et al, Grüner and Huber, Müller-Buschbaum et al, Gutsche et al, Braunmüller et al, Laube et al, Brücker, Nottebrock et al

  14. Modification of Glucose Oxidase biofuel cell by multi-walled carbon nanotubes

    Science.gov (United States)

    Lotfi, Ladan; Farahbakhsh, Afshin; Aghili, Sina

    2018-01-01

    Biofuel cells are a subset of fuel cells that employ biocatalysts. Enzyme-based biofuel cells (EBFCs) generate electrical energy from biofuels such as glucose and ethanol, which are renewable and sustainable energy sources. Glucose biofuel cells (GBFCs) are particularly interesting nowadays due to continuous harvesting of oxygen and glucose from bioavailable substrates, activity inside the human body, and environmental benign, which generate electricity through oxidation of glucose on the anode and reduction of oxygen on the cathode. Promoting the electron transfer of redox enzymes at modified electrode utilizing Nano size materials, such as carbon nanotubes (CNT), to achieve the direct electrochemistry of enzymes has been reported. The polypyrrole-MWCNTs-glucose oxidase (PY-CNT-GOx) electrode has been investigated in the present work. Cyclic voltammetry tests were performed in a three-electrode electrochemical set-up with modified electrode (Pt/PPy/MWCNTs/GOx) was used as working electrode. Platinum flat and Ag/AgCl (saturated KCl) were used as counter electrode and the reference electrode, respectively. The biofuel cells probe was prepared by immobilizing MWCNTs at the tip of a platinum (Pt) electrode (0.5 cm2) with PPy as the support matrix We have demonstrated a well-dispersed nanomaterial PPy/MWNT, which is able to immobilize GOx firmly under the condition of the absence of any other cross-linking agent.

  15. Microfluidic device, and related methods

    Science.gov (United States)

    Wong, Eric W. (Inventor)

    2010-01-01

    A method of making a microfluidic device is provided. The method features patterning a permeable wall on a substrate, and surrounding the permeable wall with a solid, non-permeable boundary structure to establish a microfluidic channel having a cross-sectional dimension less than 5,000 microns and a cross-sectional area at least partially filled with the permeable wall so that fluid flowing through the microfluidic channel at least partially passes through the permeable wall.

  16. International Trade of Biofuels (Brochure)

    Energy Technology Data Exchange (ETDEWEB)

    2013-05-01

    In recent years, the production and trade of biofuels has increased to meet global demand for renewable fuels. Ethanol and biodiesel contribute much of this trade because they are the most established biofuels. Their growth has been aided through a variety of policies, especially in the European Union, Brazil, and the United States, but ethanol trade and production have faced more targeted policies and tariffs than biodiesel. This fact sheet contains a summary of the trade of biofuels among nations, including historical data on production, consumption, and trade.

  17. Methods of making microfluidic devices

    KAUST Repository

    Buttner, Ulrich

    2017-06-01

    Microfluidics has advanced in terms of designs and structures, however, fabrication methods are either time consuming or expensive to produce, in terms of the facilities and equipment needed. A fast and economically viable method is provided to allow, for example, research groups to have access to microfluidic fabrication. Unlike most fabrication methods, a method is provided to fabricate a microfluidic device in one step. In an embodiment, a resolution of 50 micrometers was achieved by using maskless high-resolution digital light projection (MDLP). Bonding and channel fabrication of complex or simple structures can be rapidly incorporated to fabricate the microfluidic devices.

  18. The Microfluidic Jukebox

    Science.gov (United States)

    Tan, Say Hwa; Maes, Florine; Semin, Benoît; Vrignon, Jérémy; Baret, Jean-Christophe

    2014-04-01

    Music is a form of art interweaving people of all walks of life. Through subtle changes in frequencies, a succession of musical notes forms a melody which is capable of mesmerizing the minds of people. With the advances in technology, we are now able to generate music electronically without relying solely on physical instruments. Here, we demonstrate a musical interpretation of droplet-based microfluidics as a form of novel electronic musical instruments. Using the interplay of electric field and hydrodynamics in microfluidic devices, well controlled frequency patterns corresponding to musical tracks are generated in real time. This high-speed modulation of droplet frequency (and therefore of droplet sizes) may also provide solutions that reconciles high-throughput droplet production and the control of individual droplet at production which is needed for many biochemical or material synthesis applications.

  19. Microfluidic redox battery.

    Science.gov (United States)

    Lee, Jin Wook; Goulet, Marc-Antoni; Kjeang, Erik

    2013-07-07

    A miniaturized microfluidic battery is proposed, which is the first membraneless redox battery demonstrated to date. This unique concept capitalizes on dual-pass flow-through porous electrodes combined with stratified, co-laminar flow to generate electrical power on-chip. The fluidic design is symmetric to allow for both charging and discharging operations in forward, reverse, and recirculation modes. The proof-of-concept device fabricated using low-cost materials integrated in a microfluidic chip is shown to produce competitive power levels when operated on a vanadium redox electrolyte. A complete charge/discharge cycle is performed to demonstrate its operation as a rechargeable battery, which is an important step towards providing sustainable power to lab-on-a-chip and microelectronic applications.

  20. Microfluidic Biochip Design

    Science.gov (United States)

    Panzarella, Charles

    2004-01-01

    As humans prepare for the exploration of our solar system, there is a growing need for miniaturized medical and environmental diagnostic devices for use on spacecrafts, especially during long-duration space missions where size and power requirements are critical. In recent years, the biochip (or Lab-on-a- Chip) has emerged as a technology that might be able to satisfy this need. In generic terms, a biochip is a miniaturized microfluidic device analogous to the electronic microchip that ushered in the digital age. It consists of tiny microfluidic channels, pumps and valves that transport small amounts of sample fluids to biosensors that can perform a variety of tests on those fluids in near real time. It has the obvious advantages of being small, lightweight, requiring less sample fluids and reagents and being more sensitive and efficient than larger devices currently in use. Some of the desired space-based applications would be to provide smaller, more robust devices for analyzing blood, saliva and urine and for testing water and food supplies for the presence of harmful contaminants and microorganisms. Our group has undertaken the goal of adapting as well as improving upon current biochip technology for use in long-duration microgravity environments. In addition to developing computational models of the microfluidic channels, valves and pumps that form the basis of every biochip, we are also trying to identify potential problems that could arise in reduced gravity and develop solutions to these problems. One such problem is due to the prevalence of bubbly sample fluids in microgravity. A bubble trapped in a microfluidic channel could be detrimental to the operation of a biochip. Therefore, the process of bubble formation in microgravity needs to be studied, and a model of this process has been developed and used to understand how bubbles develop and move through biochip components. It is clear that some type of bubble filter would be necessary in Space, and

  1. Droplet based microfluidics

    International Nuclear Information System (INIS)

    Seemann, Ralf; Brinkmann, Martin; Pfohl, Thomas; Herminghaus, Stephan

    2012-01-01

    Droplet based microfluidics is a rapidly growing interdisciplinary field of research combining soft matter physics, biochemistry and microsystems engineering. Its applications range from fast analytical systems or the synthesis of advanced materials to protein crystallization and biological assays for living cells. Precise control of droplet volumes and reliable manipulation of individual droplets such as coalescence, mixing of their contents, and sorting in combination with fast analysis tools allow us to perform chemical reactions inside the droplets under defined conditions. In this paper, we will review available drop generation and manipulation techniques. The main focus of this review is not to be comprehensive and explain all techniques in great detail but to identify and shed light on similarities and underlying physical principles. Since geometry and wetting properties of the microfluidic channels are crucial factors for droplet generation, we also briefly describe typical device fabrication methods in droplet based microfluidics. Examples of applications and reaction schemes which rely on the discussed manipulation techniques are also presented, such as the fabrication of special materials and biophysical experiments.

  2. A Microfluidic Cell Concentrator

    Science.gov (United States)

    Warrick, Jay; Casavant, Ben; Frisk, Megan; Beebe, David

    2010-01-01

    Cell concentration via centrifugation is a ubiquitous step in many cell culture procedures. At the macroscale, centrifugation suffers from a number of limitations particularly when dealing with small numbers of cells (e.g., less than 50,000). On the other hand, typical microscale methods for cell concentration can affect cell physiology and bias readouts of cell behavior and function. In this paper, we present a microfluidic concentrator device that utilizes the effects of gravity to allow cells to gently settle out of a suspension into a collection region without the use of specific adhesion ligands. Dimensional analysis was performed to compare different device designs and was verified with flow modeling to optimize operational parameters. We are able to concentrate low-density cell suspensions in a microfluidic chamber, achieving a cell loss of only 1.1 ± 0.6% (SD, n=7) with no observed loss during a subsequent cell staining protocol which incorporates ~36 complete device volume replacements. This method provides a much needed interface between rare cell samples and microfluidic culture assays. PMID:20843010

  3. Microfluidics with fluid walls.

    Science.gov (United States)

    Walsh, Edmond J; Feuerborn, Alexander; Wheeler, James H R; Tan, Ann Na; Durham, William M; Foster, Kevin R; Cook, Peter R

    2017-10-10

    Microfluidics has great potential, but the complexity of fabricating and operating devices has limited its use. Here we describe a method - Freestyle Fluidics - that overcomes many key limitations. In this method, liquids are confined by fluid (not solid) walls. Aqueous circuits with any 2D shape are printed in seconds on plastic or glass Petri dishes; then, interfacial forces pin liquids to substrates, and overlaying an immiscible liquid prevents evaporation. Confining fluid walls are pliant and resilient; they self-heal when liquids are pipetted through them. We drive flow through a wide range of circuits passively by manipulating surface tension and hydrostatic pressure, and actively using external pumps. Finally, we validate the technology with two challenging applications - triggering an inflammatory response in human cells and chemotaxis in bacterial biofilms. This approach provides a powerful and versatile alternative to traditional microfluidics.The complexity of fabricating and operating microfluidic devices limits their use. Walsh et al. describe a method in which circuits are printed as quickly and simply as writing with a pen, and liquids in them are confined by fluid instead of solid walls.

  4. Microfluidic desalination : capacitive deionization on chip for microfluidic sample preparation

    NARCIS (Netherlands)

    Roelofs, Susan Helena

    2015-01-01

    The main aim of the work described in this thesis is to implement the desalination technique capacitive deionization (CDI) on a microfluidic chip to improve the reproducibility in the analysis of biological samples for drug development. Secondly, microfluidic CDI allows for the in situ study of ion

  5. Sustainability aspects of biofuel production

    Science.gov (United States)

    Pawłowski, L.; Cel, W.; Wójcik Oliveira, K.

    2018-05-01

    Nowadays, world development depends on the energy supply. The use of fossil fuels leads to two threats: depletion of resources within a single century and climate changes caused by the emission of CO2 from fossil fuels combustion. Widespread application of renewable energy sources, in which biofuels play a major role, is proposed as a counter-measure. The paper made an attempt to evaluate to what extent biofuels meet the criteria of sustainable development. It was shown that excessive development of biofuels may threaten the sustainable development paradigms both in the aspect of: intergenerational equity, leading to an increase of food prices, as well as intergenerational equity, resulting in degradation of the environment. The paper presents the possibility of sustainable biofuels production increase.

  6. Biofuels: policies, standards and technologies

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-09-15

    Skyrocketing prices of crude oil in the middle of the first decade of the 21st century accompanied by rising prices for food focused political and public attention on the role of biofuels. On the one hand, biofuels were considered as a potential automotive fuel with a bright future, on the other hand, biofuels were accused of competing with food production for land. The truth must lie somewhere in-between and is strongly dependent on the individual circumstance in different countries and regions. As food and energy are closely interconnected and often compete with each other for other resources, such as water, the World Energy Council - following numerous requests of its Member Committees - decided to undertake an independent assessment of biofuels policies, technologies and standards.

  7. Conventional and advanced liquid biofuels

    Directory of Open Access Journals (Sweden)

    Đurišić-Mladenović Nataša L.

    2016-01-01

    Full Text Available Energy security and independence, increase and fluctuation of the oil price, fossil fuel resources depletion and global climate change are some of the greatest challanges facing societies today and in incoming decades. Sustainable economic and industrial growth of every country and the world in general requires safe and renewable resources of energy. It has been expected that re-arrangement of economies towards biofuels would mitigate at least partially problems arised from fossil fuel consumption and create more sustainable development. Of the renewable energy sources, bioenergy draws major and particular development endeavors, primarily due to the extensive availability of biomass, already-existence of biomass production technologies and infrastructure, and biomass being the sole feedstock for liquid fuels. The evolution of biofuels is classified into four generations (from 1st to 4th in accordance to the feedstock origin; if the technologies of feedstock processing are taken into account, than there are two classes of biofuels - conventional and advanced. The conventional biofuels, also known as the 1st generation biofuels, are those produced currently in large quantities using well known, commercially-practiced technologies. The major feedstocks for these biofuels are cereals or oleaginous plants, used also in the food or feed production. Thus, viability of the 1st generation biofuels is questionable due to the conflict with food supply and high feedstocks’ cost. This limitation favoured the search for non-edible biomass for the production of the advanced biofuels. In a general and comparative way, this paper discusses about various definitions of biomass, classification of biofuels, and brief overview of the biomass conversion routes to liquid biofuels depending on the main constituents of the biomass. Liquid biofuels covered by this paper are those compatible with existing infrastructure for gasoline and diesel and ready to be used in

  8. Alternative Crops and Biofuel Production

    Energy Technology Data Exchange (ETDEWEB)

    Kenkel, Philip [Oklahoma State Univ., Stillwater, OK (United States); Holcomb, Rodney B. [Oklahoma State Univ., Stillwater, OK (United States)

    2013-03-01

    In order for the biofuel industry to meet the RFS benchmarks for biofuels, new feedstock sources and production systems will have to be identified and evaluated. The Southern Plains has the potential to produce over a billion gallons of biofuels from regionally produced alternative crops, agricultural residues, and animal fats. While information on biofuel conversion processes is available, it is difficult for entrepreneurs, community planners and other interested individuals to determine the feasibility of biofuel processes or to match production alternatives with feed stock availability and community infrastructure. This project facilitates the development of biofuel production from these regionally available feed stocks. Project activities are concentrated in five major areas. The first component focused on demonstrating the supply of biofuel feedstocks. This involves modeling the yield and cost of production of dedicated energy crops at the county level. In 1991 the DOE selected switchgrass as a renewable source to produce transportation fuel after extensive evaluations of many plant species in multiple location (Caddel et al,. 2010). However, data on the yield and cost of production of switchgrass are limited. This deficiency in demonstrating the supply of biofuel feedstocks was addressed by modeling the potential supply and geographic variability of switchgrass yields based on relationship of available switchgrass yields to the yields of other forage crops. This model made it possible to create a database of projected switchgrass yields for five different soil types at the county level. A major advantage of this methodology is that the supply projections can be easily updated as improved varieties of switchgrass are developed and additional yield data becomes available. The modeling techniques are illustrated using the geographic area of Oklahoma. A summary of the regional supply is then provided.

  9. Effect of biofuel on environment

    International Nuclear Information System (INIS)

    Kalam, M.A; Masjuki, H.H.; Maleque, M.A.

    2001-01-01

    Biofuels are alcohols, esters, and other chemical made from cellulosic biomass such as herbaceous and woody plants, agricultural and forestry residues, and a large portion of municipal solid and industrial waste. Biofuels are renewable and mostly suitable for diesel engines due to their similar physiochemical properties as traditional diesel oil. Demand of biofuel is increasing and some European countries have started using biofuel in diesel engine. This interest has been grown in many countries mainly due to fluctuating oil prices because of diminishing availability of conventional sources and polluted environment. However, the use of biofuel for diesel engine would be more beneficial to oil importing countries by saving foreign exchange, because biofuel is domestic renewable fuels. This paper presents the evaluation results of a multi-cylinder diesel engine operated on blends of ten, twenty, thirty, forty and fifty percent of ordinary coconut oil (COCO) with ordinary diesel (OD). The test results from all the COCO blends were compared with OD. The fuels were compared based on the emissions results including, exhaust temperature, NO x , smoke, CO, HC, benzene and polycyclic aromatic hydrocarbon (PAH). Carbon deposit on injector nozzles was also monitored. Exhaust emissions results showed that increasing coconut oil in blend decreases all the exhaust emissions. Carbon deposited on injector nozzles was observed where no hard carbon was found on injector tip when the engine was running on COCO blends. (Author)

  10. MICROFLUIDIC COMPONENT CAPABLE OF SELF-SEALING

    DEFF Research Database (Denmark)

    2009-01-01

    A microfluidic component (100) for building a microfluidic system is provided. The microfluidic component (100) can be mounted on a microf luidic breadboard (202) in a manner that allows it to be connected to other microfluidic components (204, 206) without the requirement of additional devices....... The microfluidic component (100) comprises at least one flexible tube piece (102) for transporting a fluid. The microfluidic component (100) also comprises means for applying and maintaining pressure (104) between the flexible tube piece (102) and a tube piece (208, 210) housed in another microfluidic component...

  11. Biofuels feedstock development program

    International Nuclear Information System (INIS)

    Wright, L.L.; Cushman, J.H.; Ehrenshaft, A.R.; McLaughlin, S.B.; McNabb, W.A.; Martin, S.A.; Ranney, J.W.; Tuskan, G.A.; Turhollow, A.F.

    1993-11-01

    The Department of Energy's (DOE's) Biofuels Feedstock Development Program (BFDP) leads the nation in the research, development, and demonstration of environmentally acceptable and commercially viable dedicated feedstock supply systems (DFSS). The purpose of this report is to highlight the status and accomplishments of the research that is currently being funded by the BFDP. Highlights summarized here and additional accomplishments are described in more detail in the sections associated with each major program task. A few key accomplishments include (1) development of a methodology for doing a cost-supply analysis for energy crops and the application of that methodology to looking at possible land use changes around a specific energy facility in East Tennessee; (2) preliminary documentation of the relationship between woody crop plantation locations and bird diversity at sites in the Midwest, Canada, and the pacific Northwest supplied indications that woody crop plantations could be beneficial to biodiversity; (3) the initiation of integrated switchgrass variety trials, breeding research, and biotechnology research for the south/southeast region; (4) development of a data base management system for documenting the results of herbaceous energy crop field trials; (5) publication of three issues of Energy Crops Forum and development of a readership of over 2,300 individuals or organizations as determined by positive responses on questionnaires

  12. BIOFUEL FROM CORN STOVER

    Directory of Open Access Journals (Sweden)

    Ljiljanka Tomerlin

    2003-12-01

    Full Text Available This paper deals with production of ethyl alcohol (biofuel from corn stover acid hydrolysate by yeasts, respectively at Pichia stipitis y-7124 and Pachysolen tannophilus y-2460 and Candida shehatae y-12856. Since moist corn stover (Hybryds 619 is proving to decomposition by phyllospheric microflora. It was (conserved spattered individually by microbicids: Busan-90, Izosan-G and formalin. In form of prismatic bales, it was left in the open air during 6 months (Octobar - March. At the beginning and after 6 months the microbiological control was carried out. The only one unspattered (control and three stover corn bals being individually spattered by microbicids were fragmented and cooked with sulfur acid. The obtained four acid hydrolysates are complex substratums, containing, apart from the sugars (about 11 g dm-3 pentosa and about 5.4 g dm-3 hexose, decomposite components as lignin, caramel sugars and uronic acids. By controlling the activity of the mentioned yeasts it was confirmed that yeasts Pichia stipitis y-7124 obtained best capability of ethyl alcohol production from corn stover acid hydrolysate at 0.23 vol. % to 0.49 vol. %.

  13. Biofuels and food security

    Directory of Open Access Journals (Sweden)

    Dmitry S. STREBKOV

    2015-03-01

    Full Text Available The major source of energy comes from fossil fuels. The current situation in the field of fuel and energy is becoming more problematic as world population continues to grow because of the limitation of fossil fuels reserve and its pressure on environment. This review aims to find economic, reliable, renewable and non-polluting energy sources to reduce high energy tariffs in Russian Federation. Biofuel is fuel derived directly from plants, or indirectly from agricultural, commercial, domestic, and/or industrial wastes. Other alternative energy sources including solar energy and electric power generation are also discussed. Over 100 Mt of biomass available for energy purposes is produced every year in Russian. One of the downsides of biomass energy is its potential threatens to food security and forage industries. An innovative approach proved that multicomponent fuel (80% diesel oil content for motor and 64% for in stove fuel can remarkably reduce the costs. This paper proposed that the most promising energy model for future is based on direct solar energy conversion and transcontinental terawatt power transmission with the use of resonant wave-guide technology.

  14. Sustainable Biofuels Development Center

    Energy Technology Data Exchange (ETDEWEB)

    Reardon, Kenneth F. [Colorado State Univ., Fort Collins, CO (United States)

    2015-03-01

    The mission of the Sustainable Bioenergy Development Center (SBDC) is to enhance the capability of America’s bioenergy industry to produce transportation fuels and chemical feedstocks on a large scale, with significant energy yields, at competitive cost, through sustainable production techniques. Research within the SBDC is organized in five areas: (1) Development of Sustainable Crops and Agricultural Strategies, (2) Improvement of Biomass Processing Technologies, (3) Biofuel Characterization and Engine Adaptation, (4) Production of Byproducts for Sustainable Biorefining, and (5) Sustainability Assessment, including evaluation of the ecosystem/climate change implication of center research and evaluation of the policy implications of widespread production and utilization of bioenergy. The overall goal of this project is to develop new sustainable bioenergy-related technologies. To achieve that goal, three specific activities were supported with DOE funds: bioenergy-related research initiation projects, bioenergy research and education via support of undergraduate and graduate students, and Research Support Activities (equipment purchases, travel to attend bioenergy conferences, and seminars). Numerous research findings in diverse fields related to bioenergy were produced from these activities and are summarized in this report.

  15. biofuel development in California

    Directory of Open Access Journals (Sweden)

    Varaprasad Bandaru

    2015-07-01

    Full Text Available Biofuels are expected to play a major role in meeting California's long-term energy needs, but many factors influence the commercial viability of the various feedstock and production technology options. We developed a spatially explicit analytic framework that integrates models of plant growth, crop adoption, feedstock location, transportation logistics, economic impact, biorefinery costs and biorefinery energy use and emissions. We used this framework to assess the economic potential of hybrid poplar as a feedstock for jet fuel production in Northern California. Results suggest that the region has sufficient suitable croplands (2.3 million acres and nonarable lands (1.5 million acres for poplar cultivation to produce as much as 2.26 billion gallons of jet fuel annually. However, there are major obstacles to such large-scale production, including, on nonarable lands, low poplar yields and broad spatial distribution and, on croplands, competition with existing crops. We estimated the production cost of jet fuel to be $4.40 to $5.40 per gallon for poplar biomass grown on nonarable lands and $3.60 to $4.50 per gallon for biomass grown on irrigated cropland; the current market price is $2.12 per gallon. Improved poplar yields, use of supplementary feedstocks at the biorefinery and economic supports such as carbon credits could help to overcome these barriers.

  16. Biofuels and sustainability in Africa

    Energy Technology Data Exchange (ETDEWEB)

    Amigun, Bamikole; Stafford, William [Sustainable Energy Futures, Natural Resources and the Environment, Council for Scientific and Industrial Research (CSIR), 7599 Stellenbosch (South Africa); Musango, Josephine Kaviti [Resource Based Sustainable Development, Natural Resources and the Environment, Council for Scientific and Industrial Research (CSIR), 7599 Stellenbosch (South Africa)

    2011-02-15

    The combined effects of climate change, the continued volatility of fuel prices, the recent food crisis and global economic turbulence have triggered a sense of urgency among policymakers, industries and development practitioners to find sustainable and viable solutions in the area of biofuels. This sense of urgency is reflected in the rapid expansion of global biofuels production and markets over the past few years. Biofuels development offers developing countries some prospect of self-reliant energy supplies at national and local levels, with potential economic, ecological, social, and security benefits. Forty-two African countries are net oil importers. This makes them particularly vulnerable to volatility in global fuel prices and dependent on foreign exchange to cover their domestic energy needs. The goal therefore is to reduce the high dependence on imported petroleum by developing domestic, renewable energy. But can this objective be achieved while leaving a minimal social and environmental footprint? A fundamental question is if biofuels can be produced with consideration of social, economic and environmental factors without setting unrealistic expectation for an evolving renewable energy industry that holds such great promise. The overall performance of different biofuels in reducing non-renewable energy use and greenhouse gas emissions varies when considering the entire lifecycle from production through to use. The net performance depends on the type of feedstock, the production process and the amount of non-renewable energy needed. This paper presents an overview of the development of biofuels in Africa, and highlights country-specific economic, environmental and social issues. It proposes a combination framework of policy incentives as a function of technology maturity, discusses practices, processes and technologies that can improve efficiency, lower energy and water demand, and further reduce the social and environmental footprint of biofuels

  17. Biofuels and sustainability in Africa

    International Nuclear Information System (INIS)

    Amigun, Bamikole; Stafford, William; Musango, Josephine Kaviti

    2011-01-01

    The combined effects of climate change, the continued volatility of fuel prices, the recent food crisis and global economic turbulence have triggered a sense of urgency among policymakers, industries and development practitioners to find sustainable and viable solutions in the area of biofuels. This sense of urgency is reflected in the rapid expansion of global biofuels production and markets over the past few years. Biofuels development offers developing countries some prospect of self-reliant energy supplies at national and local levels, with potential economic, ecological, social, and security benefits. Forty-two African countries are net oil importers. This makes them particularly vulnerable to volatility in global fuel prices and dependent on foreign exchange to cover their domestic energy needs. The goal therefore is to reduce the high dependence on imported petroleum by developing domestic, renewable energy. But can this objective be achieved while leaving a minimal social and environmental footprint? A fundamental question is if biofuels can be produced with consideration of social, economic and environmental factors without setting unrealistic expectation for an evolving renewable energy industry that holds such great promise. The overall performance of different biofuels in reducing non-renewable energy use and greenhouse gas emissions varies when considering the entire lifecycle from production through to use. The net performance depends on the type of feedstock, the production process and the amount of non-renewable energy needed. This paper presents an overview of the development of biofuels in Africa, and highlights country-specific economic, environmental and social issues. It proposes a combination framework of policy incentives as a function of technology maturity, discusses practices, processes and technologies that can improve efficiency, lower energy and water demand, and further reduce the social and environmental footprint of biofuels

  18. Microfluidic Cell Culture Device

    Science.gov (United States)

    Takayama, Shuichi (Inventor); Cabrera, Lourdes Marcella (Inventor); Heo, Yun Seok (Inventor); Smith, Gary Daniel (Inventor)

    2014-01-01

    Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.

  19. Microfluidic Scintillation Detectors

    CERN Multimedia

    Microfluidic scintillation detectors are devices of recent introduction for the detection of high energy particles, developed within the EP-DT group at CERN. Most of the interest for such technology comes from the use of liquid scintillators, which entails the possibility of changing the active material in the detector, leading to an increased radiation resistance. This feature, together with the high spatial resolution and low thickness deriving from the microfabrication techniques used to manufacture such devices, is desirable not only in instrumentation for high energy physics experiments but also in medical detectors such as beam monitors for hadron therapy.

  20. Spatial manipulation with microfluidics

    Directory of Open Access Journals (Sweden)

    Benjamin eLin

    2015-04-01

    Full Text Available Biochemical gradients convey information through space, time, and concentration, and are ultimately capable of spatially resolving distinct cellular phenotypes, such as differentiation, proliferation, and migration. How these gradients develop, evolve, and function during development, homeostasis, and various disease states is a subject of intense interest across a variety of disciplines. Microfluidic technologies have become essential tools for investigating gradient sensing in vitro due to their ability to precisely manipulate fluids on demand in well controlled environments at cellular length scales. This minireview will highlight their utility for studying gradient sensing along with relevant applications to biology.

  1. Assessment of pelletized biofuels

    International Nuclear Information System (INIS)

    Samson, R.; Duxbury, P.; Drisdelle, M.; Lapointe, C.

    2000-04-01

    There has been an increased interest in the development of economical and convenient renewable energy fuels, resulting from concerns about climate change and rising oil prices. An opportunity to use agricultural land as a means of producing renewable fuels in large quantities, relying on wood and agricultural residues only has come up with recent advances in biomass feedstock development and conversion technologies. Increasing carbon storage in the landscape and displacing fossil fuels in combustion applications can be accomplished by using switchgrass and short rotation willow which abate greenhouse gas emissions. The potential of switchgrass and short rotation willow, as well as other biomass residues as new feedstocks for the pellet industry is studied in this document. Higher throughput rates are facilitated by using switchgrass, which shows potential as a pelleting feedstock. In addition, crop drying requires less energy than wood. By taking into consideration energy for switchgrass production, transportation to the conversion facility, preprocessing, pelleting, and marketing, the overall energy balance of switchgrass is 14.5:1. Research on alfalfa pelleting can be applied to switchgrass, as both exhibit a similar behaviour. The length of chop, the application of high temperature steam and the use of a die with a suitable length/diameter ratio are all factors that contribute to the successful pelleting of switchgrass. Switchgrass has a similar combustion efficiency (82 to 84 per cent) to wood (84 to 86 per cent), as determined by combustion trials conducted by the Canada Centre for Mineral and Energy Technology (CANMET) in the Dell-Point close coupled gasifier. The energy content is 96 per cent of the energy of wood pellets on a per tonne basis. Clinker formation was observed, which necessitated some adjustments of the cleaner grate settings. While stimulating rural development and export market opportunities, the high yielding closed loop biofuels show

  2. The second generation biofuels from the biomass

    International Nuclear Information System (INIS)

    2007-01-01

    The author takes stock on the second generation biofuels in the world, the recent technologies, their advantages, the research programs and the economical and environmental impacts of the biofuels development. (A.L.B.)

  3. Microfluidic fuel cells and batteries

    CERN Document Server

    Kjeang, Erik

    2014-01-01

    Microfluidic fuel cells and batteries represent a special type of electrochemical power generators that can be miniaturized and integrated in a microfluidic chip. Summarizing the initial ten years of research and development in this emerging field, this SpringerBrief is the first book dedicated to microfluidic fuel cell and battery technology for electrochemical energy conversion and storage. Written at a critical juncture, where strategically applied research is urgently required to seize impending technology opportunities for commercial, analytical, and educational utility, the intention is

  4. Biofuels from food processing wastes.

    Science.gov (United States)

    Zhang, Zhanying; O'Hara, Ian M; Mundree, Sagadevan; Gao, Baoyu; Ball, Andrew S; Zhu, Nanwen; Bai, Zhihui; Jin, Bo

    2016-04-01

    Food processing industry generates substantial high organic wastes along with high energy uses. The recovery of food processing wastes as renewable energy sources represents a sustainable option for the substitution of fossil energy, contributing to the transition of food sector towards a low-carbon economy. This article reviews the latest research progress on biofuel production using food processing wastes. While extensive work on laboratory and pilot-scale biosystems for energy production has been reported, this work presents a review of advances in metabolic pathways, key technical issues and bioengineering outcomes in biofuel production from food processing wastes. Research challenges and further prospects associated with the knowledge advances and technology development of biofuel production are discussed. Copyright © 2016. Published by Elsevier Ltd.

  5. Panorama 2007: Biofuels in Europe

    International Nuclear Information System (INIS)

    Prieur-Vernat, A.; His, St.

    2007-01-01

    The current leader on the world bio-diesel market, Europe is, after the United States and Brazil, one of the regions driving the production and utilization of biofuels. Its ambitious bio-fuel content targets for motor fuels (5.75% by 2010 and 8% by 2015) encourage Member States to significantly develop those pathways. This raises certain questions, especially about available biomass resources. It is likely that, beyond 2010, technologies other than those in existence today, using ligno-cellulosic biomass, will have to be implemented. (author)

  6. Policies promoting Biofuels in Sweden

    Energy Technology Data Exchange (ETDEWEB)

    Holmgren, Kristina [IVL Swedish Environmental Research Inst., Goeteborg (Sweden); Chalmers Univ. of Technology, Div. of Heat and Power Technology., Goeteborg (Sweden)

    2012-07-01

    This report was written as part of a course in Environmental Economics and Policy Instruments at the University of Gothenburg. It aims at summarizing the policy instruments introduced to directly affect the production and use of biofuels in Sweden. Since Sweden is part of the EU also EU policies were included. There are additional policy instruments which affect the production and utilization of biofuels in a more indirect way that are not presented here. The economic analysis in this paper is limited and could be developed from the information presented in order to draw further conclusions on necessary changes in order to reach set targets.

  7. Evaluation of the effects of enzyme-based liquid chemical stabilizers on subgrade soils

    CSIR Research Space (South Africa)

    Mgangira, Martin B

    2009-07-01

    Full Text Available The purpose of this study was to asses the strength of enzyme treated soil material. Thus the aim of the paper is to present laboratory results on the effects of two enzyme-based liquid chemicals as soil stabilizers. Soil samples were prepared...

  8. A novel ethanol/oxygen microfluidic fuel cell with enzymes immobilized onto cantilevered porous electrodes

    Science.gov (United States)

    Desmaële, D.; Nguyen-Boisse, T. T.; Renaud, L.; Tingry, S.

    2016-11-01

    This paper introduces a novel design of membraneless microfluidic biofuel cell that incorporates three-dimensional porous electrodes containing immobilized enzymes to catalyze redox reactions occurring in the presence of ethanol/O2 co-laminar flows. In order to maximize the penetration depth of the reactants inside the porous medium, we report on the preliminary evaluation of cantilevered bioelectrodes, namely the fibrous electrodes protrude along the internal walls of the miniature electrochemical chamber. As a first proof-of-concept, we demonstrate the integration of a bioanode and a biocathode into a lamination-based microfluidic cell fabricated via rapid prototyping. With enzymes deposited into the fibrous structure of 25 mm long, 1 mm wide and 0.11 mm thick carbon paper electrodes, the volumetric power density reached 1.25 mW cm-3 at 0.43 V under a flow rate of 50 μL min-1. An advantage of the presented microfluidic biofuel cell is that it can be adapted to include a larger active electrode volume via the vertical stacking of multiple thin bioelectrodes. We therefore envision that our design would be amenable to reach the level of net power required to supply energy to a plurality of low-consumption electronic devices.

  9. Assessing the environmental sustainability of biofuels.

    Science.gov (United States)

    Kazamia, Elena; Smith, Alison G

    2014-10-01

    Biofuels vary in their potential to reduce greenhouse gas emissions when displacing fossil fuels. Savings depend primarily on the crop used for biofuel production, and on the effect that expanding its cultivation has on land use. Evidence-based policies should be used to ensure that maximal sustainability benefits result from the development of biofuels. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Overview on Biofuels from a European Perspective

    Science.gov (United States)

    Ponti, Luigi; Gutierrez, Andrew Paul

    2009-01-01

    In light of the recently developed European Union (EU) Biofuels Strategy, the literature is reviewed to examine (a) the coherency of biofuel production with the EU nonindustrial vision of agriculture, and (b) given its insufficient land base, the implications of a proposed bioenergy pact to grow biofuel crops in the developing world to meet EU…

  11. Biofuels, a bad thing?; Boeser Biokraftstoff?

    Energy Technology Data Exchange (ETDEWEB)

    Jensen, D.; Bensmann, M.

    2008-05-15

    The discussions over biofuels are still going on. Critics claim that biofuels ruin engine components, destroy rainforests and cause high food prices and global hunger. According to this contribution, the Federal government's biofuels policy was wrong and was doomed to fail. (orig.)

  12. Evaluation of biofuels sustainability: can we keep biofuel appropriate and green?

    CSIR Research Space (South Africa)

    Amigun, B

    2009-11-01

    Full Text Available and Industrial Research (CSIR) Pretoria, South Africa bamigun@csir.co.za Outlines • State of biofuels in Africa - Biofuels initiatives in Africa • Barriers to biofuels market penetration and policy incentives to stimulate the market. • Sustainability... are then motivated to put these ideas into practice. The end of Phase I is the political decision to invest money and other resources into biofuel research. Biofuels developmental stages in Africa…explanation © CSIR 2009 www...

  13. Biofuel supply chain, market, and policy analysis

    Science.gov (United States)

    Zhang, Leilei

    Renewable fuel is receiving an increasing attention as a substitute for fossil based energy. The US Department of Energy (DOE) has employed increasing effort on promoting the advanced biofuel productions. Although the advanced biofuel remains at its early stage, it is expected to play an important role in climate policy in the future in the transportation sector. This dissertation studies the emerging biofuel supply chain and markets by analyzing the production cost, and the outcomes of the biofuel market, including blended fuel market price and quantity, biofuel contract price and quantity, profitability of each stakeholder (farmers, biofuel producers, biofuel blenders) in the market. I also address government policy impacts on the emerging biofuel market. The dissertation is composed with three parts, each in a paper format. The first part studies the supply chain of emerging biofuel industry. Two optimization-based models are built to determine the number of facilities to deploy, facility locations, facility capacities, and operational planning within facilities. Cost analyses have been conducted under a variety of biofuel demand scenarios. It is my intention that this model will shed light on biofuel supply chain design considering operational planning under uncertain demand situations. The second part of the dissertation work focuses on analyzing the interaction between the key stakeholders along the supply chain. A bottom-up equilibrium model is built for the emerging biofuel market to study the competition in the advanced biofuel market, explicitly formulating the interactions between farmers, biofuel producers, blenders, and consumers. The model simulates the profit maximization of multiple market entities by incorporating their competitive decisions in farmers' land allocation, biomass transportation, biofuel production, and biofuel blending. As such, the equilibrium model is capable of and appropriate for policy analysis, especially for those policies

  14. Methods of making microfluidic devices

    KAUST Repository

    Buttner, Ulrich; Mashraei, Yousof; Agambayev, Sumeyra; Salama, Khaled N.

    2017-01-01

    Microfluidics has advanced in terms of designs and structures, however, fabrication methods are either time consuming or expensive to produce, in terms of the facilities and equipment needed. A fast and economically viable method is provided

  15. Microfluidic technology for molecular diagnostics.

    Science.gov (United States)

    Robinson, Tom; Dittrich, Petra S

    2013-01-01

    Molecular diagnostics have helped to improve the lives of millions of patients worldwide by allowing clinicians to diagnose patients earlier as well as providing better ongoing therapies. Point-of-care (POC) testing can bring these laboratory-based techniques to the patient in a home setting or to remote settings in the developing world. However, despite substantial progress in the field, there still remain many challenges. Progress in molecular diagnostics has benefitted greatly from microfluidic technology. This chapter aims to summarise the more recent advances in microfluidic-based molecular diagnostics. Sections include an introduction to microfluidic technology, the challenges of molecular diagnostics, how microfluidic advances are working to solve these issues, some alternative design approaches, and detection within these systems.

  16. Rapid mask prototyping for microfluidics.

    Science.gov (United States)

    Maisonneuve, B G C; Honegger, T; Cordeiro, J; Lecarme, O; Thiry, T; Fuard, D; Berton, K; Picard, E; Zelsmann, M; Peyrade, D

    2016-03-01

    With the rise of microfluidics for the past decade, there has come an ever more pressing need for a low-cost and rapid prototyping technology, especially for research and education purposes. In this article, we report a rapid prototyping process of chromed masks for various microfluidic applications. The process takes place out of a clean room, uses a commercially available video-projector, and can be completed in less than half an hour. We quantify the ranges of fields of view and of resolutions accessible through this video-projection system and report the fabrication of critical microfluidic components (junctions, straight channels, and curved channels). To exemplify the process, three common devices are produced using this method: a droplet generation device, a gradient generation device, and a neuro-engineering oriented device. The neuro-engineering oriented device is a compartmentalized microfluidic chip, and therefore, required the production and the precise alignment of two different masks.

  17. Biofuelled heating plants

    International Nuclear Information System (INIS)

    Gulliksson, Hans; Wennerstaal, L.; Zethraeus, B.; Johansson, Bert-Aake

    2001-11-01

    The purpose of this report is to serve as a basis to enable establishment and operation of small and medium-sized bio-fuel plants, district heating plants and local district heating plants. Furthermore, the purpose of this report is to serve as a guideline and basis when realizing projects, from the first concept to established plant. Taking into account all the phases, from selection of heating system, fuel type, selection of technical solutions, authorization request or application to operate a plant, planning, construction and buying, inspection, performance test, take-over and control system of the plant. Another purpose of the report is to make sure that best available technology is used and to contribute to continuous development of the technology. The report deals mainly with bio-fuelled plants in the effect range 0.3 to10 MW. The term 'plant' refers to combined power and heating plants as well as 'simpler' district heating plants. The last-mentioned is also often referred to as 'local heating plant'. In this context, the term bio fuel refers to a wide range of fuel types. The term bio fuel includes processed fractions like powders, pellets, and briquettes along with unprocessed fractions, such as by-products from the forest industry; chips and bark. Bio fuels also include straw, energy crops and cereal waste products, but these have not been expressly studied in this report. The report is structured with appendixes regarding the various phases of the projects, with the purpose of serving as a helping handbook, or manual for new establishment, helping out with technical and administrative advice and environmental requirements. Plants of this size are already expanding considerably, and the need for guiding principles for design/technology and environmental requirements is great. These guiding principles should comply with the environmental legislation requirements, and must contain advice and recommendations for bio fuel plants in this effect range, also in

  18. Ultrasensitive microfluidic solid-phase ELISA using an actuatable microwell-patterned PDMS chip.

    Science.gov (United States)

    Wang, Tanyu; Zhang, Mohan; Dreher, Dakota D; Zeng, Yong

    2013-11-07

    Quantitative detection of low abundance proteins is of significant interest for biological and clinical applications. Here we report an integrated microfluidic solid-phase ELISA platform for rapid and ultrasensitive detection of proteins with a wide dynamic range. Compared to the existing microfluidic devices that perform affinity capture and enzyme-based optical detection in a constant channel volume, the key novelty of our design is two-fold. First, our system integrates a microwell-patterned assay chamber that can be pneumatically actuated to significantly reduce the volume of chemifluorescent reaction, markedly improving the sensitivity and speed of ELISA. Second, monolithic integration of on-chip pumps and the actuatable assay chamber allow programmable fluid delivery and effective mixing for rapid and sensitive immunoassays. Ultrasensitive microfluidic ELISA was demonstrated for insulin-like growth factor 1 receptor (IGF-1R) across at least five orders of magnitude with an extremely low detection limit of 21.8 aM. The microwell-based solid-phase ELISA strategy provides an expandable platform for developing the next-generation microfluidic immunoassay systems that integrate and automate digital and analog measurements to further improve the sensitivity, dynamic ranges, and reproducibility of proteomic analysis.

  19. Passive microfluidic array card and reader

    Science.gov (United States)

    Dugan, Lawrence Christopher [Modesto, CA; Coleman, Matthew A [Oakland, CA

    2011-08-09

    A microfluidic array card and reader system for analyzing a sample. The microfluidic array card includes a sample loading section for loading the sample onto the microfluidic array card, a multiplicity of array windows, and a transport section or sections for transporting the sample from the sample loading section to the array windows. The microfluidic array card reader includes a housing, a receiving section for receiving the microfluidic array card, a viewing section, and a light source that directs light to the array window of the microfluidic array card and to the viewing section.

  20. Reconfigurable microfluidic platform in ice

    OpenAIRE

    Varejka, M.

    2008-01-01

    Microfluidic devices are popular tools in the biotechnology industry where they provide smaller reagent requirements, high speed of analysis and the possibility for automation. The aim of the project is to make a flexible biocompatible microfluidic platform adapted to different specific applications, mainly analytical and separations which parameters and configuration can be changed multiple times by changing corresponding computer programme. The current project has been sup...

  1. Enhanced Microfluidic Electromagnetic Measurements

    Science.gov (United States)

    Giovangrandi, Laurent (Inventor); Ricco, Antonio J. (Inventor); Kovacs, Gregory (Inventor)

    2015-01-01

    Techniques for enhanced microfluidic impedance spectroscopy include causing a core fluid to flow into a channel between two sheath flows of one or more sheath fluids different from the core fluid. Flow in the channel is laminar. A dielectric constant of a fluid constituting either sheath flow is much less than a dielectric constant of the core fluid. Electrical impedance is measured in the channel between at least a first pair of electrodes. In some embodiments, enhanced optical measurements include causing a core fluid to flow into a channel between two sheath flows of one or more sheath fluids different from the core fluid. An optical index of refraction of a fluid constituting either sheath flow is much less than an optical index of refraction of the core fluid. An optical property is measured in the channel.

  2. Bioanalysis in microfluidic devices.

    Science.gov (United States)

    Khandurina, Julia; Guttman, András

    2002-01-18

    Microfabricated bioanalytical devices (also referred to as laboratory-on-a-chip or micro-TAS) offer highly efficient platforms for simultaneous analysis of a large number of biologically important molecules, possessing great potential for genome, proteome and metabolome studies. Development and implementation of microfluidic-based bioanalytical tools involves both established and evolving technologies, including microlithography, micromachining, micro-electromechanical systems technology and nanotechnology. This article provides an overview of the latest developments in the key device subject areas and the basic interdisciplinary technologies. Important aspects of DNA and protein analysis, interfacing issues and system integration are all thoroughly discussed, along with applications for this novel "synergized" technology in high-throughput separations of biologically important molecules. This review also gives a better understanding of how to utilize these technologies as well as to provide appropriate technical solutions to problems perceived as being more fundamental.

  3. Estimates of US biofuels consumption, 1990

    Energy Technology Data Exchange (ETDEWEB)

    1991-10-01

    This report is the sixth in the series of publications developed by the Energy Information Administration to quantify the amount of biofuel-derived primary energy used by the US economy. It provides preliminary estimates of 1990 US biofuels energy consumption by sector and by biofuels energy resource type. The objective of this report is to provide updated annual estimates of biofuels energy consumption for use by congress, federal and state agencies, and other groups involved in activities related to the use of biofuels. 5 figs., 10 tabs.

  4. Estimates of US biofuels consumption, 1990

    International Nuclear Information System (INIS)

    1991-10-01

    This report is the sixth in the series of publications developed by the Energy Information Administration to quantify the amount of biofuel-derived primary energy used by the US economy. It provides preliminary estimates of 1990 US biofuels energy consumption by sector and by biofuels energy resource type. The objective of this report is to provide updated annual estimates of biofuels energy consumption for use by congress, federal and state agencies, and other groups involved in activities related to the use of biofuels. 5 figs., 10 tabs

  5. Biofuel production by recombinant microorganisms

    Science.gov (United States)

    Liao, James C.; Atsumi, Shota; Cann, Anthony F.

    2017-07-04

    Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate.

  6. An Outlook on Microalgal Biofuels

    NARCIS (Netherlands)

    Wijffels, R.H.; Barbosa, M.J.

    2010-01-01

    Microalgae are considered one of the most promising feedstocks for biofuels. The productivity of these photosynthetic microorganisms in converting carbon dioxide into carbon-rich lipids, only a step or two away from biodiesel, greatly exceeds that of agricultural oleaginous crops, without competing

  7. Biofuels for automobiles - an overview

    Energy Technology Data Exchange (ETDEWEB)

    Schaub, G. [Universitaet Karlsruhe, Engler-Bunte-Institut, Karlsruhe (Germany); Vetter, A. [Thueringer Landesanstalt fuer Landwirtschaft, Dornburg (Germany)

    2008-05-15

    Due to increasing oil prices and climate change concerns, biofuels have become more important as potential alternative energy sources. It is an open question as to which types of biofuels have the best yield potentials, characteristic properties and environmental consequences for providing the largest contribution to future energy requirements. Apart from the quality aspects, the question of quantity is very important, i.e., yields of biomass raw materials from agriculture and forestry as well as the conversion efficiencies/yields of the conversion process to automotive fuels. The most widely used biofuel forms today are fatty acid methyl esters and ethanol. However, in the future it is possible that synthetic hydrocarbons and hydrogen, produced via biotechnological or chemical processes may become feasible as fuel sources. Limitations in quantity are caused by net productivities of photosynthesis, which are limited by several factors, e.g., by the supply of water, limited availability of land, and conversion losses. As a consequence, biofuels as they exist can only contribute to a limited extent to securing raw material supplies for energy requirements in the future. Efficiency improvements in processing technologies and changes in consumer behavior and attitude will also be required. (Abstract Copyright [2008], Wiley Periodicals, Inc.)

  8. Employment effects of biofuels development

    International Nuclear Information System (INIS)

    Danielsson, B.O.; Hektor, B.

    1992-01-01

    Effects on employment - national and regional - from an expanding market for biofuels in Sweden are estimated in this article. The fuels considered are: Peat, straw, energy crops, silviculture, forestry waste, wood waste, by-products from paper/wood industry and processed fuels from these sources. (22 refs., tabs.)

  9. Biofuels: What potential for development?

    International Nuclear Information System (INIS)

    Alazard-Toux, Nathalie

    2010-01-01

    The current production chain of the first generation of biofuels has quite real limits. To overcome them, efforts are being made to develop processes for converting vegetable resources of little worth into fuel. This research focuses both on these resources and on the technology and processes for turning them into fuel

  10. Cadmium in the biofuel system

    International Nuclear Information System (INIS)

    Aabyhammar, T.; Fahlin, M.; Holmroos, S.

    1993-12-01

    Removal of biofuel depletes the soil of important nutrients. Investigations are being made of possibilities to return most of these nutrients by spreading the ashes remaining after combustion in the forest or on field. Return of ashes implies that both beneficial and harmful substances are returned. This study has been conducted to illustrate that the return of cadmium implies the greatest risk for negative influences. The occurrence, utilization, emissions and effects of cadmium are discussed. The behaviour of cadmium in soil is discussed in detail. Flows and quantities of cadmium in Swedish society are reviewed. Flows and quantities of both total and plant available cadmium in the entire forest and arable areas of Sweden are given. A scenario for a bioenergy system of max 100 TWh is discussed. The cadmium flow in different biofuels and forest raw products, and anticipated amounts of ashes and cadmium concentrations, are calculated. Power production from biofuels is surveyed. Possibilities to clean ashes have been examined in laboratory experiments. Ashes and trace elements occurring as a result of the gasification of biofuels are reviewed. Strategies for handling ashes are discussed. Proposals on continued inputs in both the biological and technical sciences are made. 146 refs, 23 figs, 38 tabs

  11. Digital Microfluidics Sample Analyzer

    Science.gov (United States)

    Pollack, Michael G.; Srinivasan, Vijay; Eckhardt, Allen; Paik, Philip Y.; Sudarsan, Arjun; Shenderov, Alex; Hua, Zhishan; Pamula, Vamsee K.

    2010-01-01

    Three innovations address the needs of the medical world with regard to microfluidic manipulation and testing of physiological samples in ways that can benefit point-of-care needs for patients such as premature infants, for which drawing of blood for continuous tests can be life-threatening in their own right, and for expedited results. A chip with sample injection elements, reservoirs (and waste), droplet formation structures, fluidic pathways, mixing areas, and optical detection sites, was fabricated to test the various components of the microfluidic platform, both individually and in integrated fashion. The droplet control system permits a user to control droplet microactuator system functions, such as droplet operations and detector operations. Also, the programming system allows a user to develop software routines for controlling droplet microactuator system functions, such as droplet operations and detector operations. A chip is incorporated into the system with a controller, a detector, input and output devices, and software. A novel filler fluid formulation is used for the transport of droplets with high protein concentrations. Novel assemblies for detection of photons from an on-chip droplet are present, as well as novel systems for conducting various assays, such as immunoassays and PCR (polymerase chain reaction). The lab-on-a-chip (a.k.a., lab-on-a-printed-circuit board) processes physiological samples and comprises a system for automated, multi-analyte measurements using sub-microliter samples of human serum. The invention also relates to a diagnostic chip and system including the chip that performs many of the routine operations of a central labbased chemistry analyzer, integrating, for example, colorimetric assays (e.g., for proteins), chemiluminescence/fluorescence assays (e.g., for enzymes, electrolytes, and gases), and/or conductometric assays (e.g., for hematocrit on plasma and whole blood) on a single chip platform.

  12. Parallel imaging microfluidic cytometer.

    Science.gov (United States)

    Ehrlich, Daniel J; McKenna, Brian K; Evans, James G; Belkina, Anna C; Denis, Gerald V; Sherr, David H; Cheung, Man Ching

    2011-01-01

    By adding an additional degree of freedom from multichannel flow, the parallel microfluidic cytometer (PMC) combines some of the best features of fluorescence-activated flow cytometry (FCM) and microscope-based high-content screening (HCS). The PMC (i) lends itself to fast processing of large numbers of samples, (ii) adds a 1D imaging capability for intracellular localization assays (HCS), (iii) has a high rare-cell sensitivity, and (iv) has an unusual capability for time-synchronized sampling. An inability to practically handle large sample numbers has restricted applications of conventional flow cytometers and microscopes in combinatorial cell assays, network biology, and drug discovery. The PMC promises to relieve a bottleneck in these previously constrained applications. The PMC may also be a powerful tool for finding rare primary cells in the clinic. The multichannel architecture of current PMC prototypes allows 384 unique samples for a cell-based screen to be read out in ∼6-10 min, about 30 times the speed of most current FCM systems. In 1D intracellular imaging, the PMC can obtain protein localization using HCS marker strategies at many times for the sample throughput of charge-coupled device (CCD)-based microscopes or CCD-based single-channel flow cytometers. The PMC also permits the signal integration time to be varied over a larger range than is practical in conventional flow cytometers. The signal-to-noise advantages are useful, for example, in counting rare positive cells in the most difficult early stages of genome-wide screening. We review the status of parallel microfluidic cytometry and discuss some of the directions the new technology may take. Copyright © 2011 Elsevier Inc. All rights reserved.

  13. Four myths surrounding U.S. biofuels

    International Nuclear Information System (INIS)

    Wetzstein, M.; Wetzstein, H.

    2011-01-01

    The rapid growth of biofuels has elicited claims and predictions concerning the current and future role of these fuels in the U.S. vehicle-fuel portfolio. These assertions are at times based on a false set of assumptions concerning the biofuel's market related to the petroleum and agricultural commodities markets, and the nonmarket consequences of our automobile driving. As an aid in clarifying these market relations, the following four biofuel myths are presented: (1) biofuels will be adopted because we will soon run out of oil, (2) biofuels will solve the major external costs associated with our automobile driving, (3) biofuels cause food price inflation (the food before fuel issue), and (4) biofuels will become a major vehicle fuel. - Highlights: → Biofuels will be adopted because we will soon run out of oil. → Biofuels will solve the major external costs associated with our automobile driving. → Biofuels cause food price inflation (the food before fuel issue). → Biofuels will become a major vehicle fuel.

  14. Biofuels Refining Engineering

    Energy Technology Data Exchange (ETDEWEB)

    Lobban, Lance [Univ. of Oklahoma, Norman, OK (United States)

    2017-03-28

    The goal of this project is the development of novel catalysts and knowledge of reaction pathways and mechanisms for conversion of biomass-based compounds to fuels that are compatible with oil-based fuels and with acceptable or superior fuel properties. The research scope included both catalysts to convert lignocellulosic biomass-based molecules (from pyrolysis) and vegetable oil-based molecules (i.e., triglycerides and fatty acid methyl esters). This project comprised five technical tasks. Each task is briefly introduced below, and major technical accomplishments summarized. Technical accomplishments were described in greater detail in the quarterly progress reports, and in even more detail in the >50 publications acknowledging this DoE project funding (list of publications and presentations included at the end of this report). The results of this research added greatly to the knowledge base necessary for upgrading of pyrolysis oil to hydrocarbon fuels and chemicals, and for conversion of vegetable oils to fungible diesel fuel. Numerous new catalysts and catalytic reaction systems were developed for upgrading particular compounds or compound families found in the biomass-based pyrolysis oils and vegetable oils. Methods to mitigate catalyst deactivation were investigated, including novel reaction/separation systems. Performance and emission characteristics of biofuels in flames and engines were measured. Importantly, the knowledge developed from this project became the basis for a subsequent collaborative proposal led by our research group, involving researchers from the University of Wisconsin, the University of Pittsburg, and the Idaho National Lab, for the DoE Carbon, Hydrogen and Separations Efficiency (CHASE) program, which was subsequently funded (one of only four projects awarded in the CHASE program). The CHASE project examined novel catalytic processes for lignocellulosic biomass conversion as well as technoeconomic analyses for process options for maximum

  15. Integrated lenses in polystyrene microfluidic devices

    KAUST Repository

    Fan, Yiqiang; Li, Huawei; Foulds, Ian G.

    2013-01-01

    This paper reports a new method for integrating microlenses into microfluidic devices for improved observation. Two demonstration microfluidic devices were provided which were fabricated using this new technique. The integrated microlenses were

  16. Manipulation of microfluidic droplets by electrorheological fluid

    KAUST Repository

    Zhang, Menying; Gong, Xiuqing; Wen, Weijia

    2009-01-01

    Microfluidics, especially droplet microfluidics, attracts more and more researchers from diverse fields, because it requires fewer materials and less time, produces less waste and has the potential of highly integrated and computer

  17. Microfluidic standardization: Past, present and future

    NARCIS (Netherlands)

    Heeren, H. van; Atkins, T.; Blom, M.; Bullema, J.E.; Tantra, R.; Verhoeven, D.; Verplanck, N.

    2016-01-01

    This paper addresses the issue of standardization in microfluidics. It contains the main points of an industry wide agreement about microfluidic port pitches and port nomenclature. It also addresses device classification and future steps.

  18. Review of Microfluidic Photobioreactor Technology for Metabolic Engineering and Synthetic Biology of Cyanobacteria and Microalgae

    Directory of Open Access Journals (Sweden)

    Ya-Tang Yang

    2016-10-01

    Full Text Available One goal of metabolic engineering and synthetic biology for cyanobacteria and microalgae is to engineer strains that can optimally produce biofuels and commodity chemicals. However, the current workflow is slow and labor intensive with respect to assembly of genetic parts and characterization of production yields because of the slow growth rates of these organisms. Here, we review recent progress in the microfluidic photobioreactors and identify opportunities and unmet needs in metabolic engineering and synthetic biology. Because of the unprecedented experimental resolution down to the single cell level, long-term real-time monitoring capability, and high throughput with low cost, microfluidic photobioreactor technology will be an indispensible tool to speed up the development process, advance fundamental knowledge, and realize the full potential of metabolic engineering and synthetic biology for cyanobacteria and microalgae.

  19. A model-based framework for design of intensified enzyme-based processes

    DEFF Research Database (Denmark)

    Román-Martinez, Alicia

    This thesis presents a generic and systematic model-based framework to design intensified enzyme-based processes. The development of the presented methodology was motivated by the needs of the bio-based industry for a more systematic approach to achieve intensification in its production plants...... in enzyme-based processes which have found significant application in the pharmaceutical, food, and renewable fuels sector. The framework uses model-based strategies for (bio)-chemical process design and optimization, including the use of a superstructure to generate all potential reaction......(s)-separation(s) options according to a desired performance criteria and a generic mathematical model represented by the superstructure to derive the specific models corresponding to a specific process option. In principle, three methods of intensification of bioprocess are considered in this thesis: 1. enzymatic one...

  20. Catalytic Enzyme-Based Methods for Water Treatment and Water Distribution System Decontamination. 1. Literature Survey

    Science.gov (United States)

    2006-06-01

    best examples of this is glucose isomerase, which has been used in the commercial production of high fructose corn syrup (HFCS) since 1967.230 Most...EDGEWOOD CHEMICAL BIOLOGICAL CENTER U.S. ARMY RESEARCH, DEVELOPMENT AND ENGINEERING COMMAND ECBC-TR-489 CATALYTIC ENZYME-BASED METHODS FOR WATER ...TREATMENT AND WATER DISTRIBUTION SYSTEM DECONTAMINATION 1. LITERATURE SURVEY Joseph J. DeFrank RESEARCH AND TECHNOLOGY DIRECTORATE June 2006 Approved for

  1. Total employment effect of biofuels

    International Nuclear Information System (INIS)

    Stridsberg, S.

    1998-08-01

    The study examined the total employment effect of both direct production of biofuel and energy conversion to heat and electricity, as well as the indirect employment effect arising from investments and other activities in conjunction with the production organization. A secondary effect depending on the increased capital flow is also included in the final result. The scenarios are based on two periods, 1993-2005 and 2005-2020. In the present study, the different fuels and the different applications have been analyzed individually with regard to direct and indirect employment within each separate sector. The greatest employment effect in the production chain is shown for logging residues with 290 full-time jobs/TWh, whereas other biofuels range between 80 and 280 full-time jobs/TWh. In the processing chain, the corresponding range is 200-300 full-time jobs per each additional TWh. Additionally and finally, there are secondary effects that give a total of 650 full-time jobs/TWh. Together with the predicted increase, this suggests that unprocessed fuel will provide an additional 16 000 annual full-time jobs, and that fuel processing will contribute with a further 5 000 full-time jobs. The energy production from the fuels will provide an additional 13 000 full-time jobs. The total figure of 34 000 annual full-time jobs must then be reduced by about 4000 on account of lost jobs, mainly in the oil sector and to some extent in imports of biofuel. In addition, the anticipated increase in capital turnover that occurs within the biofuel sector, will increase full-time jobs up to year 2020. Finally, a discussion is given of the accomplishment of the programmes anticipated by the scenario, where it is noted that processing of biofuel to wafers, pellets or powder places major demands on access to raw material of good quality and that agrarian fuels must be given priority if they are to enter the system sufficiently fast. Straw is already a resource but is still not accepted by

  2. From first generation biofuels to advanced solar biofuels.

    Science.gov (United States)

    Aro, Eva-Mari

    2016-01-01

    Roadmaps towards sustainable bioeconomy, including the production of biofuels, in many EU countries mostly rely on biomass use. However, although biomass is renewable, the efficiency of biomass production is too low to be able to fully replace the fossil fuels. The use of land for fuel production also introduces ethical problems in increasing the food price. Harvesting solar energy by the photosynthetic machinery of plants and autotrophic microorganisms is the basis for all biomass production. This paper describes current challenges and possibilities to sustainably increase the biomass production and highlights future technologies to further enhance biofuel production directly from sunlight. The biggest scientific breakthroughs are expected to rely on a new technology called "synthetic biology", which makes engineering of biological systems possible. It will enable direct conversion of solar energy to a fuel from inexhaustible raw materials: sun light, water and CO2. In the future, such solar biofuels are expected to be produced in engineered photosynthetic microorganisms or in completely synthetic living factories.

  3. Toward nitrogen neutral biofuel production.

    Science.gov (United States)

    Huo, Yi-Xin; Wernick, David G; Liao, James C

    2012-06-01

    Environmental concerns and an increasing global energy demand have spurred scientific research and political action to deliver large-scale production of liquid biofuels. Current biofuel processes and developing approaches have focused on closing the carbon cycle by biological fixation of atmospheric carbon dioxide and conversion of biomass to fuels. To date, these processes have relied on fertilizer produced by the energy-intensive Haber-Bosch process, and have not addressed the global nitrogen cycle and its environmental implications. Recent developments to convert protein to fuel and ammonia may begin to address these problems. In this scheme, recycling ammonia to either plant or algal feedstocks reduces the demand for synthetic fertilizer supplementation. Further development of this technology will realize its advantages of high carbon fixation rates, inexpensive and simple feedstock processing, in addition to reduced fertilizer requirements. Copyright © 2011 Elsevier Ltd. All rights reserved.

  4. Algal biofuels: challenges and opportunities.

    Science.gov (United States)

    Leite, Gustavo B; Abdelaziz, Ahmed E M; Hallenbeck, Patrick C

    2013-10-01

    Biodiesel production using microalgae is attractive in a number of respects. Here a number of pros and cons to using microalgae for biofuels production are reviewed. Algal cultivation can be carried out using non-arable land and non-potable water with simple nutrient supply. In addition, algal biomass productivities are much higher than those of vascular plants and the extractable content of lipids that can be usefully converted to biodiesel, triacylglycerols (TAGs) can be much higher than that of the oil seeds now used for first generation biodiesel. On the other hand, practical, cost-effective production of biofuels from microalgae requires that a number of obstacles be overcome. These include the development of low-cost, effective growth systems, efficient and energy saving harvesting techniques, and methods for oil extraction and conversion that are environmentally benign and cost-effective. Promising recent advances in these areas are highlighted. Copyright © 2013 Elsevier Ltd. All rights reserved.

  5. Health effects of biofuel exhaust

    OpenAIRE

    Vugt, M.A.T.M. van; Mulderij, M.; Usta, M.; Kadijk, G.; Kooter, I.M.; Krul, C.A.M.

    2009-01-01

    Alternatives to fossil fuels receive a lot of attention. In particular, oil derived of specific crops forms a promising fuel. In order to warrant global expectance of such novel fuels, safety issues associated with combustion of these fuels needs to be assessed. Although only a few public reports exist, recently potential toxic effects associated with biofuels has been published. Here, we report the analysis of a comprehensive study, comparing the toxic effects of conventional diesel, biodies...

  6. Microfluidic stretchable RF electronics.

    Science.gov (United States)

    Cheng, Shi; Wu, Zhigang

    2010-12-07

    Stretchable electronics is a revolutionary technology that will potentially create a world of radically different electronic devices and systems that open up an entirely new spectrum of possibilities. This article proposes a microfluidic based solution for stretchable radio frequency (RF) electronics, using hybrid integration of active circuits assembled on flex foils and liquid alloy passive structures embedded in elastic substrates, e.g. polydimethylsiloxane (PDMS). This concept was employed to implement a 900 MHz stretchable RF radiation sensor, consisting of a large area elastic antenna and a cluster of conventional rigid components for RF power detection. The integrated radiation sensor except the power supply was fully embedded in a thin elastomeric substrate. Good electrical performance of the standalone stretchable antenna as well as the RF power detection sub-module was verified by experiments. The sensor successfully detected the RF radiation over 5 m distance in the system demonstration. Experiments on two-dimensional (2D) stretching up to 15%, folding and twisting of the demonstrated sensor were also carried out. Despite the integrated device was severely deformed, no failure in RF radiation sensing was observed in the tests. This technique illuminates a promising route of realizing stretchable and foldable large area integrated RF electronics that are of great interest to a variety of applications like wearable computing, health monitoring, medical diagnostics, and curvilinear electronics.

  7. Land availability for biofuel production.

    Science.gov (United States)

    Cai, Ximing; Zhang, Xiao; Wang, Dingbao

    2011-01-01

    Marginal agricultural land is estimated for biofuel production in Africa, China, Europe, India, South America, and the continental United States, which have major agricultural production capacities. These countries/regions can have 320-702 million hectares of land available if only abandoned and degraded cropland and mixed crop and vegetation land, which are usually of low quality, are accounted. If grassland, savanna, and shrubland with marginal productivity are considered for planting low-input high-diversity (LIHD) mixtures of native perennials as energy crops, the total land availability can increase from 1107-1411 million hectares, depending on if the pasture land is discounted. Planting the second generation of biofuel feedstocks on abandoned and degraded cropland and LIHD perennials on grassland with marginal productivity may fulfill 26-55% of the current world liquid fuel consumption, without affecting the use of land with regular productivity for conventional crops and without affecting the current pasture land. Under the various land use scenarios, Africa may have more than one-third, and Africa and Brazil, together, may have more than half of the total land available for biofuel production. These estimations are based on physical conditions such as soil productivity, land slope, and climate.

  8. Land Clearing and the Biofuel Carbon Debt

    Science.gov (United States)

    Fargione, Joseph; Hill, Jason; Tilman, David; Polasky, Stephen; Hawthorne, Peter

    2008-02-01

    Increasing energy use, climate change, and carbon dioxide (CO2) emissions from fossil fuels make switching to low-carbon fuels a high priority. Biofuels are a potential low-carbon energy source, but whether biofuels offer carbon savings depends on how they are produced. Converting rainforests, peatlands, savannas, or grasslands to produce food crop based biofuels in Brazil, Southeast Asia, and the United States creates a “biofuel carbon debt” by releasing 17 to 420 times more CO2 than the annual greenhouse gas (GHG) reductions that these biofuels would provide by displacing fossil fuels. In contrast, biofuels made from waste biomass or from biomass grown on degraded and abandoned agricultural lands planted with perennials incur little or no carbon debt and can offer immediate and sustained GHG advantages.

  9. How policies affect international biofuel price linkages

    International Nuclear Information System (INIS)

    Rajcaniova, Miroslava; Drabik, Dusan; Ciaian, Pavel

    2013-01-01

    We estimate the role of biofuel policies in determining which country is the price leader in world biofuel markets using a cointegration analysis and a Vector Error Correction (VEC) model. Weekly prices are analyzed for the EU, US, and Brazilian ethanol and biodiesel markets in the 2002–2010 and 2005–2010 time periods, respectively. The US blender's tax credit and Brazil's consumer tax exemption are found to play a role in determining the ethanol prices in other countries. For biodiesel, our results demonstrate that EU policies – the consumer tax exemption and blending target – tend to determine the world biodiesel price. - Highlights: • We estimate the role of biofuel policies in determining biofuel prices. • We use a cointegration analysis and the Vector Error Correction (VEC) model. • The biofuel policies in US and Brazil determine the world ethanol prices. • EU biofuel policies tend to form the world biodiesel price

  10. Frames in the Ethiopian Debate on Biofuels

    Directory of Open Access Journals (Sweden)

    Brigitte Portner

    2013-01-01

    Full Text Available Biofuel production, while highly contested, is supported by a number of policies worldwide. Ethiopia was among the first sub-Saharan countries to devise a biofuel policy strategy to guide the associated demand toward sustainable development. In this paper, I discuss Ethiopia’s biofuel policy from an interpretative research position using a frames approach and argue that useful insights can be obtained by paying more attention to national contexts and values represented in the debates on whether biofuel production can or will contribute to sustainable development. To this end, I was able to distinguish three major frames used in the Ethiopian debate on biofuels: an environmental rehabilitation frame, a green revolution frame and a legitimacy frame. The article concludes that actors advocating for frames related to social and human issues have difficulties entering the debate and forming alliances, and that those voices need to be included in order for Ethiopia to develop a sustainable biofuel sector.

  11. Promoting biofuels: Implications for developing countries

    International Nuclear Information System (INIS)

    Peters, Joerg; Thielmann, Sascha

    2008-01-01

    Interest in biofuels is growing worldwide as concerns about the security of energy supply and climate change are moving into the focus of policy makers. With the exception of bioethanol from Brazil, however, production costs of biofuels are typically much higher than those of fossil fuels. As a result, promotion measures such as tax exemptions or blending quotas are indispensable for ascertaining substantial biofuel demand. With particular focus on developing countries, this paper discusses the economic justification of biofuel promotion instruments and investigates their implications. Based on data from India and Tanzania, we find that substantial biofuel usage induces significant financial costs. Furthermore, acreage availability is a binding natural limitation that could also lead to conflicts with food production. Yet, if carefully implemented under the appropriate conditions, biofuel programs might present opportunities for certain developing countries

  12. Microfluidic Technologies for Synthetic Biology

    Directory of Open Access Journals (Sweden)

    Sung Kuk Lee

    2011-06-01

    Full Text Available Microfluidic technologies have shown powerful abilities for reducing cost, time, and labor, and at the same time, for increasing accuracy, throughput, and performance in the analysis of biological and biochemical samples compared with the conventional, macroscale instruments. Synthetic biology is an emerging field of biology and has drawn much attraction due to its potential to create novel, functional biological parts and systems for special purposes. Since it is believed that the development of synthetic biology can be accelerated through the use of microfluidic technology, in this review work we focus our discussion on the latest microfluidic technologies that can provide unprecedented means in synthetic biology for dynamic profiling of gene expression/regulation with high resolution, highly sensitive on-chip and off-chip detection of metabolites, and whole-cell analysis.

  13. Oil price, biofuels and food supply

    International Nuclear Information System (INIS)

    Timilsina, Govinda R.; Mevel, Simon; Shrestha, Ashish

    2011-01-01

    The price of oil could play a significant role in influencing the expansion of biofuels, but this issue has yet to be fully investigated in the literature. Using a global computable general equilibrium (CGE) model, this study analyzes the impact of oil price on biofuel expansion, and subsequently, on food supply. The study shows that a 65% increase in oil price in 2020 from the 2009 level would increase the global biofuel penetration to 5.4% in 2020 from 2.4% in 2009. If oil prices rise 150% from their 2009 levels by 2020, the resulting penetration of biofuels would be 9%, which is higher than that would be caused by current mandates and targets introduced in more than forty countries around the world. The study also shows that aggregate agricultural output drops due to an oil price increase, but the drop is small in major biofuel producing countries as the expansion of biofuels would partially offset the negative impacts of the oil price increase on agricultural outputs. An increase in oil price would reduce global food supply through direct impacts as well as through the diversion of food commodities and cropland towards the production of biofuels. - Highlights: ► A global CGE model to analyze impacts of oil price on biofuels and food supply. ► Global biofuel penetration increases from 2.4% (2009) to 5.4% (2020) in baseline. ► A 150% rise of oil price boosts biofuels more than current mandates and targets do. ► Biofuels partially offset drops in agricultural outputs caused by oil price rise. ► Biofuels as well as oil price rise negatively affect global food supply.

  14. Biofuels in Italy: obstacles and development opportunities

    International Nuclear Information System (INIS)

    Pignatelli, Vito; Clementi, Chiara

    2006-01-01

    Today biofuels are the sole realistically practical way to reduce CO 2 emissions in the transportation sector. In many countries, including Italy, biofuel production and use are already a reality corresponding to a large agro-industrial production system that uses essentially mature technologies. To significantly lower production costs and optimise land use, Italy needs to develop new, second-generation biofuel production operations that can offer significant opportunities to the nation's agro-industrial sector [it

  15. DLA Energy Biofuel Feedstock Metrics Study

    Science.gov (United States)

    2012-12-11

    moderately/highly in- vasive  Metric 2: Genetically modified organism ( GMO ) hazard, Yes/No and Hazard Category  Metric 3: Species hybridization...4– biofuel distribution Stage # 5– biofuel use Metric 1: State inva- siveness ranking Yes Minimal Minimal No No Metric 2: GMO hazard Yes...may utilize GMO microbial or microalgae species across the applicable biofuel life cycles (stages 1–3). The following consequence Metrics 4–6 then

  16. Spontaneous oscillations in microfluidic networks

    Science.gov (United States)

    Case, Daniel; Angilella, Jean-Regis; Motter, Adilson

    2017-11-01

    Precisely controlling flows within microfluidic systems is often difficult which typically results in systems being heavily reliant on numerous external pumps and computers. Here, I present a simple microfluidic network that exhibits flow rate switching, bistablity, and spontaneous oscillations controlled by a single pressure. That is, by solely changing the driving pressure, it is possible to switch between an oscillating and steady flow state. Such functionality does not rely on external hardware and may even serve as an on-chip memory or timing mechanism. I use an analytic model and rigorous fluid dynamics simulations to show these results.

  17. Microfluidic device for drug delivery

    Science.gov (United States)

    Beebe, David J. (Inventor); MacDonald, Michael J. (Inventor); Eddington, David T. (Inventor); Mensing, Glennys A. (Inventor)

    2010-01-01

    A microfluidic device is provided for delivering a drug to an individual. The microfluidic device includes a body that defines a reservoir for receiving the drug therein. A valve interconnects the reservoir to an output needle that is insertable into the skin of an individual. A pressure source urges the drug from the reservoir toward the needle. The valve is movable between a closed position preventing the flow of the drug from the reservoir to the output needle and an open position allowing for the flow of the drug from the reservoir to the output needle in response to a predetermined condition in the physiological fluids of the individual.

  18. Strategic niche management for biofuels : analysing past experiments for developing new biofuels policy

    NARCIS (Netherlands)

    Laak, W.W.M.; Raven, R.P.J.M.; Verbong, G.P.J.

    2007-01-01

    Biofuels have gained a lot of attention since the implementation of the 2003 European Directive on biofuels. In the Netherlands the contribution of biofuels is still very limited despite several experiments in the past. This article aims to contribute to the development of successful policies for

  19. [Biofuels, food security and transgenic crops].

    Science.gov (United States)

    Acosta, Orlando; Chaparro-Giraldo, Alejandro

    2009-01-01

    Soaring global food prices are threatening to push more poor people back below the poverty line; this will probably become aggravated by the serious challenge that increasing population and climate changes are posing for food security. There is growing evidence that human activities involving fossil fuel consumption and land use are contributing to greenhouse gas emissions and consequently changing the climate worldwide. The finite nature of fossil fuel reserves is causing concern about energy security and there is a growing interest in the use of renewable energy sources such as biofuels. There is growing concern regarding the fact that biofuels are currently produced from food crops, thereby leading to an undesirable competition for their use as food and feed. Nevertheless, biofuels can be produced from other feedstocks such as lingo-cellulose from perennial grasses, forestry and vegetable waste. Biofuel energy content should not be exceeded by that of the fossil fuel invested in its production to ensure that it is energetically sustainable; however, biofuels must also be economically competitive and environmentally acceptable. Climate change and biofuels are challenging FAO efforts aimed at eradicating hunger worldwide by the next decade. Given that current crops used in biofuel production have not been domesticated for this purpose, transgenic technology can offer an enormous contribution towards improving biofuel crops' environmental and economic performance. The present paper critically presents some relevant relationships between biofuels, food security and transgenic plant technology.

  20. Towards Sustainable Production of Biofuels from Microalgae

    Directory of Open Access Journals (Sweden)

    Hans Ragnar Giselrød

    2008-07-01

    Full Text Available Renewable and carbon neutral biofuels are necessary for environmental and economic sustainability. The viability of the first generation biofuels production is however questionable because of the conflict with food supply. Microalgal biofuels are a viable alternative. The oil productivity of many microalgae exceeds the best producing oil crops. This paper aims to analyze and promote integration approaches for sustainable microalgal biofuel production to meet the energy and environmental needs of the society. The emphasis is on hydrothermal liquefaction technology for direct conversion of algal biomass to liquid fuel.

  1. Bridging Flows: Microfluidic End‐User Solutions

    DEFF Research Database (Denmark)

    Sabourin, David

    Microfluidic applications hold promise for many different end‐users both within and outside, and across many different research communities. Despite the benefits of microfluidic approaches, adoption and implementation thereof is often hindered by practical issues. Microfluidic components which......‐integrated interconnection and miniaturized peristaltic pump solutions were then combined into modular microfluidic systems. One system provides high interconnection numbers/density and allows many possible configurations. Additionally, and apart from many other accounts of modular microfluidic solutions, methods...... for control and actuation of microfluidic networks built from the modular components is described. Prototypes of the microfluidic system have begun to be distributed to external collaborators and researcher parties. These end‐users will assist in the validation of the approach and ultimately fulfil the key...

  2. Dual-nozzle microfluidic droplet generator

    Science.gov (United States)

    Choi, Ji Wook; Lee, Jong Min; Kim, Tae Hyun; Ha, Jang Ho; Ahrberg, Christian D.; Chung, Bong Geun

    2018-05-01

    The droplet-generating microfluidics has become an important technique for a variety of applications ranging from single cell analysis to nanoparticle synthesis. Although there are a large number of methods for generating and experimenting with droplets on microfluidic devices, the dispensing of droplets from these microfluidic devices is a challenge due to aggregation and merging of droplets at the interface of microfluidic devices. Here, we present a microfluidic dual-nozzle device for the generation and dispensing of uniform-sized droplets. The first nozzle of the microfluidic device is used for the generation of the droplets, while the second nozzle can accelerate the droplets and increase the spacing between them, allowing for facile dispensing of droplets. Computational fluid dynamic simulations were conducted to optimize the design parameters of the microfluidic device.

  3. Cross-linked glucose oxidase clusters for biofuel cell anode catalysts

    International Nuclear Information System (INIS)

    Dudzik, Jonathan; Audette, Gerald F; Chang, Wen-Chi; Kannan, A M; Filipek, Slawomir; Viswanathan, Sowmya; Li, Pingzuo; Renugopalakrishnan, V

    2013-01-01

    The efficient localization of increased levels of active enzymes onto conducting scaffolds is important for the development of enzyme-based biofuel cells. Cross-linked enzyme clusters (CEC) of glucose oxidase (GOx) constrained to functionalized carbon nanotubes (CEC-CNTs) were generated in order to evaluate the potential of using CECs for developing GOx-based bioanodes functioning via direct electron transfer from the GOx active site to the CNT scaffold. CEC-CNTs generated from several weight-to-weight ratios of GOx:CNT were examined for comparable catalytic activity to free GOx into the solution, with CEC-CNTs generated from a 100% GOx solution displaying the greatest enzymatic activity. Scanning transmission electron microscopic analysis of CEC-CNTs generated from 100% GOx to CNT (wt/wt) ratios revealed that CEC clusters of ∼78 µm 2 localized to the CNT surface. Electrochemical analysis indicates that the enzyme is engaged in direct electron transfer, and biofuel cells generated using GOx CEC-CNT bioanodes were observed to have a peak power density of ∼180 µW cm −2 . These data indicate that the generation of nano-to-micro-sized active enzyme clusters is an attractive option for the design of enzyme-specific biofuel cell powered implantable devices. (paper)

  4. Integrated microfluidic probe station.

    Science.gov (United States)

    Perrault, C M; Qasaimeh, M A; Brastaviceanu, T; Anderson, K; Kabakibo, Y; Juncker, D

    2010-11-01

    The microfluidic probe (MFP) consists of a flat, blunt tip with two apertures for the injection and reaspiration of a microjet into a solution--thus hydrodynamically confining the microjet--and is operated atop an inverted microscope that enables live imaging. By scanning across a surface, the microjet can be used for surface processing with the capability of both depositing and removing material; as it operates under immersed conditions, sensitive biological materials and living cells can be processed. During scanning, the MFP is kept immobile and centered over the objective of the inverted microscope, a few micrometers above a substrate that is displaced by moving the microscope stage and that is flushed continuously with the microjet. For consistent and reproducible surface processing, the gap between the MFP and the substrate, the MFP's alignment, the scanning speed, the injection and aspiration flow rates, and the image capture need all to be controlled and synchronized. Here, we present an automated MFP station that integrates all of these functionalities and automates the key operational parameters. A custom software program is used to control an independent motorized Z stage for adjusting the gap, a motorized microscope stage for scanning the substrate, up to 16 syringe pumps for injecting and aspirating fluids, and an inverted fluorescence microscope equipped with a charge-coupled device camera. The parallelism between the MFP and the substrate is adjusted using manual goniometer at the beginning of the experiment. The alignment of the injection and aspiration apertures along the scanning axis is performed using a newly designed MFP screw holder. We illustrate the integrated MFP station by the programmed, automated patterning of fluorescently labeled biotin on a streptavidin-coated surface.

  5. Comprehensive evaluation of SNP identification with the Restriction Enzyme-based Reduced Representation Library (RRL method

    Directory of Open Access Journals (Sweden)

    Du Ye

    2012-02-01

    Full Text Available Abstract Background Restriction Enzyme-based Reduced Representation Library (RRL method represents a relatively feasible and flexible strategy used for Single Nucleotide Polymorphism (SNP identification in different species. It has remarkable advantage of reducing the complexity of the genome by orders of magnitude. However, comprehensive evaluation for actual efficacy of SNP identification by this method is still unavailable. Results In order to evaluate the efficacy of Restriction Enzyme-based RRL method, we selected Tsp 45I enzyme which covers 266 Mb flanking region of the enzyme recognition site according to in silico simulation on human reference genome, then we sequenced YH RRL after Tsp 45I treatment and obtained reads of which 80.8% were mapped to target region with an 20-fold average coverage, about 96.8% of target region was covered by at least one read and 257 K SNPs were identified in the region using SOAPsnp software. Compared with whole genome resequencing data, we observed false discovery rate (FDR of 13.95% and false negative rate (FNR of 25.90%. The concordance rate of homozygote loci was over 99.8%, but that of heterozygote were only 92.56%. Repeat sequences and bases quality were proved to have a great effect on the accuracy of SNP calling, SNPs in recognition sites contributed evidently to the high FNR and the low concordance rate of heterozygote. Our results indicated that repeat masking and high stringent filter criteria could significantly decrease both FDR and FNR. Conclusions This study demonstrates that Restriction Enzyme-based RRL method was effective for SNP identification. The results highlight the important role of bias and the method-derived defects represented in this method and emphasize the special attentions noteworthy.

  6. Enhancement of ethanol-oxygen biofuel cell output using a CNT based nano-composite as bioanode.

    Science.gov (United States)

    Gouranlou, Farideh; Ghourchian, Hedayatollah

    2016-04-15

    The present research, describes preparation and application of a novel bioanode for ethanol-oxygen biofuel cells. We applied an enzyme based nanocomposite consisting of polymethylene green as electron transfer mediator, carboxylated-multiwall carbon nanotubes as electron transfer accelerator, alcohol dehydrogenase as biocatalyst and polydiallyldimethylammonium chloride as supporting agent. In the presence of β-nicotinamide adenine dinucleotide as cofactor, and ethanol as fuel, the feasibility of the bioanode for increasing the power was evaluated under the ambient conditions. In the optimum conditions the biofuel cell produced the power density of 1.713 mW cm(-2) and open circuit voltage of 0.281 V. Copyright © 2015 Elsevier B.V. All rights reserved.

  7. Strategic niche management for biofuels: Analysing past experiments for developing new biofuel policies

    International Nuclear Information System (INIS)

    Laak, W.W.M. van der; Raven, R.P.J.M.; Verbong, G.P.J.

    2007-01-01

    Biofuels have gained a lot of attention since the implementation of the 2003 European Directive on biofuels. In the Netherlands the contribution of biofuels is still very limited despite several experiments in the past. This article aims to contribute to the development of successful policies for stimulating biofuels by analysing three experiments in depth. The approach of strategic niche management (SNM) is used to explain success and failure of these projects. Based on the analysis as well as recent innovation literature we develop a list of guidelines that is important to consider when developing biofuel policies

  8. Topology optimization of microfluidic mixers

    DEFF Research Database (Denmark)

    Andreasen, Casper Schousboe; Gersborg, Allan Roulund; Sigmund, Ole

    2009-01-01

    This paper demonstrates the application of the topology optimization method as a general and systematic approach for microfluidic mixer design. The mixing process is modeled as convection dominated transport in low Reynolds number incompressible flow. The mixer performance is maximized by altering...

  9. A microfluidic device with pillars

    DEFF Research Database (Denmark)

    2014-01-01

    The invention provides a microfluidic device for mixing liquid reagents, the device comprises, a chip forming at least one reaction chamber between a bottom and a top and extending between an inlet and an outlet. To enable manufacturing from less rigid materials, the device comprises pillars...

  10. Microfluidic technology for PET radiochemistry

    International Nuclear Information System (INIS)

    Gillies, J.M.; Prenant, C.; Chimon, G.N.; Smethurst, G.J.; Dekker, B.A.; Zweit, J.

    2006-01-01

    This paper describes the first application of a microfabricated reaction system to positron emission tomography (PET) radiochemistry. We have applied microfluidic technology to synthesise PET radiopharmaceuticals using 18 F and 124 I as labels for fluorodeoxyglucose (FDG) and Annexin-V, respectively. These reactions involved established methods of nucleophilic substitution on a mannose triflate precursor and direct iodination of the protein using iodogen as an oxidant. This has demonstrated a proof of principle of using microfluidic technology to radiochemical reactions involving low and high molecular weight compounds. Using microfluidic reactions, [ 18 F]FDG was synthesised with a 50% incorporation of the available F-18 radioactivity in a very short time of 4 s. The radiolabelling efficiency of 124 I Annexin-V was 40% after 1 min reaction time. Chromatographic analysis showed that such reaction yields are comparable to conventional methods, but in a much shorter time. The yields can be further improved with more optimisation of the microfluidic device itself and its fluid mixing profiles. This demonstrates the potential for this technology to have an impact on rapid and simpler radiopharmaceutical synthesis using short and medium half-life radionuclides

  11. Microfluidic Liquid-Liquid Contactors

    Energy Technology Data Exchange (ETDEWEB)

    Mcculloch, Quinn [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-07-25

    This report describes progress made on the microfluidic contactor. A model was developed to predict its failure, a surrogate chemical system was selected to demonstrate mass transfer, and an all-optical system has been invented and implemented to monitor carryover and flowrates.

  12. Microfluidic devices for biological applications

    CSIR Research Space (South Africa)

    Potgieter, S

    2010-01-01

    Full Text Available Microfluidics is a multi-disciplinary field that deals with the behaviour, control and manipulation of fluids constrained to sub-millilitre volumes. It is proving to be a useful tool for biological studies, affording advantages such as reduced cost...

  13. Mixing in a Microfluid Device

    DEFF Research Database (Denmark)

    Hjorth, Poul G.; Deryabin, Mikhail

    Mixing of fluids in microchannels cannot rely on turbulence since the flow takes place at extremly low Reynolds numbers. Various active and passive devices have been developed to induce mixing in microfluid flow devices. We describe here a model of an active mixer where a transverse periodic flow...

  14. Producing biofuels using polyketide synthases

    Science.gov (United States)

    Katz, Leonard; Fortman, Jeffrey L; Keasling, Jay D

    2013-04-16

    The present invention provides for a non-naturally occurring polyketide synthase (PKS) capable of synthesizing a carboxylic acid or a lactone, and a composition such that a carboxylic acid or lactone is included. The carboxylic acid or lactone, or derivative thereof, is useful as a biofuel. The present invention also provides for a recombinant nucleic acid or vector that encodes such a PKS, and host cells which also have such a recombinant nucleic acid or vector. The present invention also provides for a method of producing such carboxylic acids or lactones using such a PKS.

  15. Synthetic Biology Guides Biofuel Production

    Directory of Open Access Journals (Sweden)

    Michael R. Connor

    2010-01-01

    Full Text Available The advancement of microbial processes for the production of renewable liquid fuels has increased with concerns about the current fuel economy. The development of advanced biofuels in particular has risen to address some of the shortcomings of ethanol. These advanced fuels have chemical properties similar to petroleum-based liquid fuels, thus removing the need for engine modification or infrastructure redesign. While the productivity and titers of each of these processes remains to be improved, progress in synthetic biology has provided tools to guide the engineering of these processes through present and future challenges.

  16. Zinc-Laccase Biofuel Cell

    Directory of Open Access Journals (Sweden)

    Abdul Aziz Ahmad

    2011-12-01

    Full Text Available A zinc-laccase biofuel cell adapting the zinc-air cell design features is investigated. A simple cell design configuration is employed: a membraneless single chamber and a freely suspended laccase in a quasi-neutral buffer electrolyte. The cell is characterised according to its open-circuit voltage, polarization profile, power density plot and discharge capacity at constant current. The biocatalytic role of laccase is evident from the polarization profile and power output plot. Performance comparison between a single chamber and dual chamber cell design is also presented. The biofuel cell possessed an open-circuit voltage of 1.2 V and delivered a maximum power density of 0.9 mW/cm2 at current density of 2.5 mA/cm2. These characteristics are comparable to biofuel cell utilising a much more complex system design.KEY WORDS (keyword:  Biofuel cell, Bioelectrochemical cell, Zinc anode, Laccase and Oxidoreductase.ABSTRAK: Sel bio-bahan api zink-laccase dengan adaptasi daripada ciri-ciri rekabentuk sel zink-udara telah dikaji. Sel dengan konfigurasi rekabentuk yang mudah digunapakai: ruangan tunggal tanpa membran dan laccase diampaikan secara bebas di dalam elektrolit pemampan quasi-neutral. Sel dicirikan berdasarkan voltan litar terbuka, profil polarisasi, plot ketumpatan kuasa dan kapasiti discas pada arus malar. Peranan laccase sebagai bio-pemangkin adalah amat ketara daripada profil polarisasi dan plot ketumpatan kuasa. Perbandingan prestasi di antara sel dengan rekabentuk ruangan tunggal and dwi-ruangan turut diketengahkan. Seperti dijangkakan, sel dengan rekabentuk ruangan tunggal menunjukkan kuasa keluaran yang lebih rendah jika dibandingkan dengan rekabentuk dwi-ruangan kemungkinan disebabkan fenomena cas bocor. Sel bio-bahan api ini mempunyai voltan litar terbuka 1.2 V dan memberikan ketumpatan kuasa maksima 0.9 mW/cm2 pada ketumpatan arus 2.5 mA/cm2. Ciri-ciri ini adalah sebanding dengan sel bio-bahan api yang menggunapakai rekabentuk sel

  17. Energy Primer: Solar, Water, Wind, and Biofuels.

    Science.gov (United States)

    Portola Inst., Inc., Menlo Park, CA.

    This is a comprehensive, fairly technical book about renewable forms of energy--solar, water, wind, and biofuels. The biofuels section covers biomass energy, agriculture, aquaculture, alcohol, methane, and wood. The focus is on small-scale systems which can be applied to the needs of the individual, small group, or community. More than one-fourth…

  18. Montana Advanced Biofuels Great Falls Approval

    Science.gov (United States)

    This November 20, 2015 letter from EPA approves the petition from Montana Advanced Biofuels, LLC, Great Falls facility, regarding ethanol produced through a dry mill process, qualifying under the Clean Air Act for advanced biofuel (D-code 5) and renewable

  19. Increase of the investments for the biofuels

    International Nuclear Information System (INIS)

    Jemain, A.

    2005-01-01

    With the construction for 2007 of six new units of biofuels (three bio-diesel and three bio-ethanol), France is developing its energy policy in favor of the biofuels. This decision benefits Diester and Sofiproteol industries which will invest in the development of their deposits. The enthusiasm is less for the bio-ethanol industries. (A.L.B.)

  20. Next generation of liquid biofuel production

    NARCIS (Netherlands)

    Batidzirai, B.

    2012-01-01

    More than 99% of all currently produced biofuels are classified as “first generation” (i.e. fuels produced primarily from cereals, grains, sugar crops and oil seeds) (IEA, 2008b). “Second generation” or “next generation” biofuels, on the other hand, are produced from lignocellulosic feedstocks such

  1. NREL Algal Biofuels Projects and Partnerships

    Energy Technology Data Exchange (ETDEWEB)

    2016-10-01

    This fact sheet highlights several algal biofuels research and development projects focused on improving the economics of the algal biofuels production process. These projects should serve as a foundation for the research efforts toward algae as a source of fuels and other chemicals.

  2. Sustainable production of grain crops for biofuels

    Science.gov (United States)

    Grain crops of the Gramineae are grown for their edible, starchy seeds. Their grain is used directly for human food, livestock feed, and as raw material for many industries, including biofuels. Using grain crops for non-food uses affects the amount of food available to the world. Grain-based biofuel...

  3. Biofuels. Environment, technology and food security

    International Nuclear Information System (INIS)

    Escobar, Jose C.; Lora, Electo S.; Venturini, Osvaldo J.; Yanez, Edgar E.; Castillo, Edgar F.; Almazan, Oscar

    2009-01-01

    The imminent decline of the world's oil production, its high market prices and environmental impacts have made the production of biofuels to reach unprecedent volumes over the last 10 years. This is why there have been intense debates among international organizations and political leaders in order to discuss the impacts of the biofuel use intensification. Besides assessing the causes of the rise in the demand and production of biofuels, this paper also shows the state of the art of their world's current production. It is also discussed different vegetable raw materials sources and technological paths to produce biofuels, as well as issues regarding production cost and the relation of their economic feasibility with oil international prices. The environmental impacts of programs that encourage biofuel production, farmland land requirements and the impacts on food production are also discussed, considering the life cycle analysis (LCA) as a tool. It is concluded that the rise in the use of biofuels is inevitable and that international cooperation, regulations and certification mechanisms must be established regarding the use of land, the mitigation of environmental and social impacts caused by biofuel production. It is also mandatory to establish appropriate working conditions and decent remuneration for workers of the biofuels production chain. (author)

  4. Microalgae for biofuels production and environmental applications ...

    African Journals Online (AJOL)

    This review presents the current classification of biofuels, with special focus on microalgae and their applicability for the production of biodiesel. The paper considered issues related with the processing and culturing of microalgae, for not only those that are involved in biofuel production, but as well as the possibility of their ...

  5. Modifying plants for biofuel and biomaterial production.

    Science.gov (United States)

    Furtado, Agnelo; Lupoi, Jason S; Hoang, Nam V; Healey, Adam; Singh, Seema; Simmons, Blake A; Henry, Robert J

    2014-12-01

    The productivity of plants as biofuel or biomaterial crops is established by both the yield of plant biomass per unit area of land and the efficiency of conversion of the biomass to biofuel. Higher yielding biofuel crops with increased conversion efficiencies allow production on a smaller land footprint minimizing competition with agriculture for food production and biodiversity conservation. Plants have traditionally been domesticated for food, fibre and feed applications. However, utilization for biofuels may require the breeding of novel phenotypes, or new species entirely. Genomics approaches support genetic selection strategies to deliver significant genetic improvement of plants as sources of biomass for biofuel manufacture. Genetic modification of plants provides a further range of options for improving the composition of biomass and for plant modifications to assist the fabrication of biofuels. The relative carbohydrate and lignin content influences the deconstruction of plant cell walls to biofuels. Key options for facilitating the deconstruction leading to higher monomeric sugar release from plants include increasing cellulose content, reducing cellulose crystallinity, and/or altering the amount or composition of noncellulosic polysaccharides or lignin. Modification of chemical linkages within and between these biomass components may improve the ease of deconstruction. Expression of enzymes in the plant may provide a cost-effective option for biochemical conversion to biofuel. © 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  6. Biofuels and biodiversity in South Africa

    Directory of Open Access Journals (Sweden)

    Patrick J. O’Farrell

    2011-05-01

    Full Text Available The South African government, as part of its efforts to mitigate the effects of the ongoing energy crisis, has proposed that biofuels should form an important part of the country’s energy supply. The contribution of liquid biofuels to the national fuel supply is expected to be at least 2% by 2013. The Biofuels Industrial Strategy of the Republic of South Africa of 2007 outlines key incentives for reaching this target and promoting the development of a sustainable biofuels industry. This paper discusses issues relating to this strategy as well as key drivers in biofuel processing with reference to potential impacts on South Africa’s rich biological heritage.

    Our understanding of many of the broader aspects of biofuels needs to be enhanced. We identify key areas where challenges exist, such as the link between technology, conversion processes and feedstock selection. The available and proposed processing technologies have important implications for land use and the use of different non-native plant species as desired feedstocks. South Africa has a long history of planting non-native plant species for commercial purposes, notably for commercial forestry. Valuable lessons can be drawn from this experience on mitigation against potential impacts by considering plausible scenarios and the appropriate management framework and policies. We conceptualise key issues embodied in the biofuels strategy, adapting a framework developed for assessing and quantifying impacts of invasive alien species. In so doing, we provide guidelines for minimising the potential impacts of biofuel projects on biodiversity.

  7. Biofuel investment in Tanzania: Omissions in implementation

    International Nuclear Information System (INIS)

    Habib-Mintz, Nazia

    2010-01-01

    Increasing demand for biofuels as a component of climate change mitigation, energy security, and a fossil fuel alternative attracts investors to developing countries like Tanzania. Ample unused land is critical for first generation biofuels production and an important feature to attract foreign direct investments that can contribute towards agricultural modernization and poverty reduction initiatives. Despite the economic justifications, the existing institutional and infrastructural capacities dictate the impacts of biofuels market penetrations. Furthermore, exogenous factors like global recessionary pressure depressed oil prices below the level at which biofuel production were profitable in 2007, making Tanzania's competitiveness and potential benefits questionable. This paper investigates the extent that first generation, jatropha-based biofuels industry development in Tanzania observed during fieldwork in Kisarawe and Bahi may fulfill policy objectives. This paper argues that without strong regulatory frameworks for land, investment management, and rural development, biofuel industrialization could further exacerbate poverty and food insecurity in Tanzania. The paper concludes with policy recommendations for first generation biofuel development while keeping in mind implications of second generation production. Since the topic is broad and multifaceted, a multidisciplinary approach is used that includes political, institutional, and agricultural economics to analyze and conceptualize biofuel industry development and food security.

  8. Global nitrogen requirement for increased biofuel production

    NARCIS (Netherlands)

    Flapper, Joris

    2008-01-01

    Biofuels are thought to be one of the options to substitute fossil fuels and prevent global warming by the greenhouse gas (GHG) effect as they are seen as a renewable form of energy. However, biofuels are almost solely subjected to criticism from an energ

  9. Scope of algae as third generation biofuels

    Directory of Open Access Journals (Sweden)

    Shuvashish eBehera

    2015-02-01

    Full Text Available An initiative has been taken to develop different solid, liquid and gaseous biofuels as the alternative energy resources. The current research and technology based on the third generation biofuels derived from algal biomass have been considered as the best alternative bioresource that avoids the disadvantages of first and second generation biofuels. Algal biomass have been investigated for the implementation of economic conversion processes producing different biofuels such as biodiesel, bioethanol, biogas, biohydrogen and other valuable co-products. In the present review, the recent findings and advance developments in algal biomass for improved biofuel production. This review discusses about the importance of the algal cell contents, various strategies for product formation through various conversion technologies, and its future scope as an energy security.

  10. Coupling of Algal Biofuel Production with Wastewater

    Directory of Open Access Journals (Sweden)

    Neha Chamoli Bhatt

    2014-01-01

    Full Text Available Microalgae have gained enormous consideration from scientific community worldwide emerging as a viable feedstock for a renewable energy source virtually being carbon neutral, high lipid content, and comparatively more advantageous to other sources of biofuels. Although microalgae are seen as a valuable source in majority part of the world for production of biofuels and bioproducts, still they are unable to accomplish sustainable large-scale algal biofuel production. Wastewater has organic and inorganic supplements required for algal growth. The coupling of microalgae with wastewater is an effective way of waste remediation and a cost-effective microalgal biofuel production. In this review article, we will primarily discuss the possibilities and current scenario regarding coupling of microalgal cultivation with biofuel production emphasizing recent progress in this area.

  11. Which future for aviation bio-fuels?

    International Nuclear Information System (INIS)

    Botti, Jean; Combarnous, Michel; Jarry, Bruno; Monsan, Pierre; Burzynski, Jean-Pierre; Jeuland, Nicolas; Porot, Pierre; Demoment, Pascale; Gillmann, Marc; Marchand, Philippe; Kuentzmann, Paul; Kurtsoglou, Nicolas; Lombaert-Valot, Isabelle; Pelegrin, Marc; Renvier, Jacques; Rousseau, Julien; Stadler, Thierry; Tremeau, Benoit

    2014-01-01

    This collective report proposes a detailed overview of the evolution of aviation fuels and bio-fuels from technological, regulatory and economic points of view. It also proposes a road-map for possible future evolutions, and outlines the different assessments between American and European countries regarding the predictions for the beginning of industrial production and use of bio-jet-fuel. After having recalled international objectives, an overview of European and French commitments for technological and operational advances, and a discussion of the role of bio-fuels in the carbon cycle, the report presents various technical constraints met in aircraft industry and describes the role bio-fuels may have. The next part proposes an overview of bio-fuels which are industrially produced in the world in 2013. The authors then focus on aviation bio-fuels (main production processes, thermo-chemical processes), discuss the political context, and examine obstacles, partnerships and the role of public authorities

  12. Second generation biofuels: Economics and policies

    International Nuclear Information System (INIS)

    Carriquiry, Miguel A.; Du Xiaodong; Timilsina, Govinda R.

    2011-01-01

    This study reviews economics of production of second generation biofuels from various feedstocks, including crop and wood/forestry residues, lignocellulosic energy crops, jatropha, and algae. The study indicates that while second generation biofuels could significantly contribute to the future energy supply mix, cost is a major barrier to its commercial production in the near to medium term. Depending upon type of biofuels, feedstock prices and conversion costs, the cost of cellulosic ethanol is found to be two to three times higher than the current price of gasoline on an energy equivalent basis. The median cost (across the studies reviewed) of biodiesel produced from microalgae, a prospective feedstock, is seven times higher than the current price of diesel, although much higher cost estimates have been reported. As compared with the case of first generation biofuels, in which feedstock can account for over two-thirds of the total costs, the share of feedstock in the total costs is relatively lower (30-50%) in the case of second generation biofuels. While significant cost reductions are needed for both types of second generation biofuels, the critical barriers are at different steps of the production process. For cellulosic ethanol, the biomass conversion costs needs to be reduced. On the other hand, feedstock cost is the main issue for biodiesel. At present, policy instruments, such as fiscal incentives and consumption mandates have in general not differentiated between the first and second generation biofuels except in the cases of the US and EU. The policy regime should be revised to account for the relative merits of different types of biofuels. - Highlights: → Second generation biofuels could significantly contribute to the future energy supply mix. → Cost is a major barrier to its the commercial production in the near to medium term. → The policy regime should be revised to account for the relative merits of different biofuels.

  13. Second generation biofuels: Economics and policies

    Energy Technology Data Exchange (ETDEWEB)

    Carriquiry, Miguel A., E-mail: miguelc@iastate.edu [Center for Agricultural and Rural Development, Iowa State University (United States); Du Xiaodong, E-mail: xdu23@wisc.edu [Department of Agricultural and Applied Economics, University of Wisconsin-Madison (United States); Timilsina, Govinda R., E-mail: gtimilsina@worldbank.org [Development Research Group, The World Bank (United States)

    2011-07-15

    This study reviews economics of production of second generation biofuels from various feedstocks, including crop and wood/forestry residues, lignocellulosic energy crops, jatropha, and algae. The study indicates that while second generation biofuels could significantly contribute to the future energy supply mix, cost is a major barrier to its commercial production in the near to medium term. Depending upon type of biofuels, feedstock prices and conversion costs, the cost of cellulosic ethanol is found to be two to three times higher than the current price of gasoline on an energy equivalent basis. The median cost (across the studies reviewed) of biodiesel produced from microalgae, a prospective feedstock, is seven times higher than the current price of diesel, although much higher cost estimates have been reported. As compared with the case of first generation biofuels, in which feedstock can account for over two-thirds of the total costs, the share of feedstock in the total costs is relatively lower (30-50%) in the case of second generation biofuels. While significant cost reductions are needed for both types of second generation biofuels, the critical barriers are at different steps of the production process. For cellulosic ethanol, the biomass conversion costs needs to be reduced. On the other hand, feedstock cost is the main issue for biodiesel. At present, policy instruments, such as fiscal incentives and consumption mandates have in general not differentiated between the first and second generation biofuels except in the cases of the US and EU. The policy regime should be revised to account for the relative merits of different types of biofuels. - Highlights: > Second generation biofuels could significantly contribute to the future energy supply mix. > Cost is a major barrier to its the commercial production in the near to medium term. > The policy regime should be revised to account for the relative merits of different biofuels.

  14. Ice matrix in reconfigurable microfluidic systems

    Energy Technology Data Exchange (ETDEWEB)

    Bossi, A M [Department of Biotechnology, University of Verona, Strada Le Grazie 15, I-37134, Verona (Italy); Vareijka, M; Piletska, E V; Turner, A P F; Piletsky, S A [Cranfield Health, Cranfield University, Vincent Building B52, Cranfield, Bedfordshire, MK43 0AL (United Kingdom); Meglinski, I [Department of Physics, University of Otago, PO Box 56, Dunedin, 9054 (New Zealand)

    2013-07-01

    Microfluidic devices find many applications in biotechnologies. Here, we introduce a flexible and biocompatible microfluidic ice-based platform with tunable parameters and configuration of microfluidic patterns that can be changed multiple times during experiments. Freezing and melting of cavities, channels and complex relief structures created and maintained in the bulk of ice by continuous scanning of an infrared laser beam are used as a valve action in microfluidic systems. We demonstrate that pre-concentration of samples and transport of ions and dyes through the open channels created can be achieved in ice microfluidic patterns by IR laser-assisted zone melting. The proposed approach can be useful for performing separation and sensing processes in flexible reconfigurable microfluidic devices. (paper)

  15. Ice matrix in reconfigurable microfluidic systems

    International Nuclear Information System (INIS)

    Bossi, A M; Vareijka, M; Piletska, E V; Turner, A P F; Piletsky, S A; Meglinski, I

    2013-01-01

    Microfluidic devices find many applications in biotechnologies. Here, we introduce a flexible and biocompatible microfluidic ice-based platform with tunable parameters and configuration of microfluidic patterns that can be changed multiple times during experiments. Freezing and melting of cavities, channels and complex relief structures created and maintained in the bulk of ice by continuous scanning of an infrared laser beam are used as a valve action in microfluidic systems. We demonstrate that pre-concentration of samples and transport of ions and dyes through the open channels created can be achieved in ice microfluidic patterns by IR laser-assisted zone melting. The proposed approach can be useful for performing separation and sensing processes in flexible reconfigurable microfluidic devices. (paper)

  16. Ice matrix in reconfigurable microfluidic systems

    Science.gov (United States)

    Bossi, A. M.; Vareijka, M.; Piletska, E. V.; Turner, A. P. F.; Meglinski, I.; Piletsky, S. A.

    2013-07-01

    Microfluidic devices find many applications in biotechnologies. Here, we introduce a flexible and biocompatible microfluidic ice-based platform with tunable parameters and configuration of microfluidic patterns that can be changed multiple times during experiments. Freezing and melting of cavities, channels and complex relief structures created and maintained in the bulk of ice by continuous scanning of an infrared laser beam are used as a valve action in microfluidic systems. We demonstrate that pre-concentration of samples and transport of ions and dyes through the open channels created can be achieved in ice microfluidic patterns by IR laser-assisted zone melting. The proposed approach can be useful for performing separation and sensing processes in flexible reconfigurable microfluidic devices.

  17. Nanostructures for all-polymer microfluidic systems

    DEFF Research Database (Denmark)

    Matschuk, Maria; Bruus, Henrik; Larsen, Niels Bent

    2010-01-01

    antistiction coating was found to improve the replication fidelity (shape and depth) of nanoscale features substantially. Arrays of holes of 50 nm diameter/35 nm depth and 100 nm/100 nm diameter, respectively, were mass-produced in cyclic olefin copolymer (Topas 5013) by injection molding. Polymer microfluidic...... channel chip parts resulted from a separate injection molding process. The microfluidic chip part and the nanostructured chip part were successfully bonded to form a sealed microfluidic system using air plasma assisted thermal bonding....

  18. Microfluidic high gradient magnetic cell separation

    Science.gov (United States)

    Inglis, David W.; Riehn, Robert; Sturm, James C.; Austin, Robert H.

    2006-04-01

    Separation of blood cells by native susceptibility and by the selective attachment of magnetic beads has recently been demonstrated on microfluidic devices. We discuss the basic principles of how forces are generated via the magnetic susceptibility of an object and how microfluidics can be combined with micron-scale magnetic field gradients to greatly enhance in principle the fractionating power of magnetic fields. We discuss our efforts and those of others to build practical microfluidic devices for the magnetic separation of blood cells. We also discuss our attempts to integrate magnetic separation with other microfluidic features for developing handheld medical diagnostic tools.

  19. Integrated lenses in polystyrene microfluidic devices

    KAUST Repository

    Fan, Yiqiang

    2013-04-01

    This paper reports a new method for integrating microlenses into microfluidic devices for improved observation. Two demonstration microfluidic devices were provided which were fabricated using this new technique. The integrated microlenses were fabricated using a free-surface thermo-compression molding method on a polystyrene (PS) sheet which was then bonded on top of microfluidic channels as a cover plate, with the convex microlenses providing a magnified image of the channel for the easier observation of the flow in the microchannels. This approach for fabricating the integrated microlens in microfluidic devices is rapid, low cost and without the requirement of cleanroom facilities. © 2013 IEEE.

  20. Indirect land use change and biofuel policy

    International Nuclear Information System (INIS)

    Kocoloski, Matthew; Griffin, W Michael; Matthews, H Scott

    2009-01-01

    Biofuel debates often focus heavily on carbon emissions, with parties arguing for (or against) biofuels solely on the basis of whether the greenhouse gas emissions of biofuels are less than (or greater than) those of gasoline. Recent studies argue that land use change leads to significant greenhouse gas emissions, making some biofuels more carbon intensive than gasoline. We argue that evaluating the suitability and utility of biofuels or any alternative energy source within the limited framework of plus and minus carbon emissions is too narrow an approach. Biofuels have numerous impacts, and policy makers should seek compromises rather than relying solely on carbon emissions to determine policy. Here, we estimate that cellulosic ethanol, despite having potentially higher life cycle CO 2 emissions (including from land use) than gasoline, would still be cost-effective at a CO 2 price of $80 per ton or less, well above estimated CO 2 mitigation costs for many alternatives. As an example of the broader approach to biofuel policy, we suggest the possibility of using the potential cost reductions of cellulosic ethanol relative to gasoline to balance out additional carbon emissions resulting from indirect land use change as an example of ways in which policies could be used to arrive at workable solutions.

  1. Assessment of Peruvian biofuel resources and alternatives

    Energy Technology Data Exchange (ETDEWEB)

    Harper, J.P.; Smith, W.; Mariani, E.

    1979-08-01

    Comprehensive assessment of the biofuel potential of Peru is based on: determination of current biofuel utilization practices, evauation of Peruvian biomass productivity, identification of Peruvian agricultural and forestry resources, assessment of resource development and management concerns, identification of market considerations, description of biofuel technological options, and identification of regional biofuel technology applications. Discussion of current biofuel utilization centers on a qualitative description of the main conversion approaches currently being practiced in Peru. Biomass productivity evaluations consider the terrain and soil, and climatic conditions found in Peru. The potential energy from Peruvian agricultural and forestry resources is described quantitatively. Potental regional production of agricultural residues and forest resources that could supply energy are identified. Assessment of resource development and management concerns focuses on harvesting, reforestation, training, and environmental consequences of utilization of forest resources. Market factors assessed include: importation, internal market development, external market development, energy policy and pricing, and transportation. Nine biofuel technology options for Peru are identified: (1) small-to-medium-scale gasification, (2) a wood waste inventory, (3) stationary and mobile charcoal production systems, (4) wood distillation, (5) forest resource development and management, (6) electrical cogeneration, (7) anaerobic digestion technology, (8) development of ethanol production capabilities, and (9) agricultural strategies for fuel production. Applications of these biofuel options are identified for each of the three major regions - nine applications for the Costa Region, eight for the Sierra Region, and ten for the Selva Region.

  2. Biofuels - Illusion or Reality? - The european experience

    International Nuclear Information System (INIS)

    Furfari, A.

    2008-01-01

    Environmental issues, rising prices and security of supply are putting energy at the centre of all attentions. Policy-makers pushed by various stakeholders are struggling to find more sustainable solutions to the world legitimate demand for energy. The transport sector is especially under pressure as it relies for 98% on oil. Despite vast research and development investments, no short-term solutions appeared to be reliable. Thanks to lawmakers support to biofuels, these substitutes for oil are now seen as the potential solution for a sustainable transport. This book analyses the real possibility of biofuels. Does Europe has enough land to produce the needed feedstock? What are the real gains in terms of greenhouse gases emissions and energy efficiency? Are biofuels really a sustainable solution? Will this policy succeed? Are the targets reachable? The reader will find some indications in this book to make up his mind on this complex, multifaceted and highly political subject. Contents: Summary. Introduction. Biofuels in the U.S.A. and Brazil. Do we have enough land in Europe? Biofuels life cycle analysis. Greenhouse gases reduction and efficiency. Case of the glycerin price. Variables affecting biofuels sustainability. Standard for Biofuels. Conclusion. General Bibliography. Annexes. References

  3. Potential of biofuels for shipping. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Florentinus, A.; Hamelinck, C.; Van den Bos, A.; Winkel, R.; Cuijpers, M. [Ecofys Netherlands, Utrecht (Netherlands)

    2012-01-15

    Biofuels could be one of the options to realize a lower carbon intensity in the propulsion of ships and also possibly reduce the effect of ship emissions on local air quality. Therefore, EMSA, the European Maritime Safety Agency, is evaluating if and how biofuels could be used in the shipping sector as an alternative fuel. To determine the potential of biofuels for ships, a clearer picture is needed on technical and organizational limitations of biofuels in ships, both on board of the ship as in the fuel supply chain to the ship. Economic and sustainability analysis of biofuels should be included in this picture, as well as an overview on current and potential policy measures to stimulate the use of biofuels in shipping. Ecofys has determined the potential of biofuels, based on analysis of collected data through literature review, own expertise and experiences, direct communication with EMSA, research publications, market developments based on press and other media, and consultations with relevant stakeholders in the shipping market.

  4. The Third Pacific Basin Biofuels Workshop: Proceedings

    Science.gov (United States)

    Among the many compelling reasons for the development of biofuels on remote Pacific islands, several of the most important include: (1) a lack of indigenous fossil fuels necessitates their import at great economic loss to local island economics, (2) ideal conditions for plant growth exist on many Pacific islands to produce yields of biomass feedstocks, (3) gaseous and liquid fuels such as methane, methanol and ethanol manufactured locally from biomass feedstocks are the most viable alternatives to gasoline and diesel fuels for transportation, and (4) the combustion of biofuels is cleaner than burning petroleum products and contributes no net atmospheric CO2 to aggravate the greenhouse effect and the subsequent threat of sea level rise to low islands. Dr. Vic Phillips, HNEI Program Manager of the Hawaii Integrated Biofuels Research Program welcomed 60 participants to the Third Pacific Basin Biofuels Workshop at the Sheraton Makaha Hotel, Waianae, Oahu, on March 27 and 28, 1989. The objectives of the workshop were to update progress since the Second Pacific Basin Biofuels Workshop in April 1987 and to develop a plan for action for biofuels R and D, technology transfer, and commercialization now (immediate attention), in the near-term (less than two years), in the mid-term (three to five years), and in the long-term (more than six years). An emerging theme of the workshop was how the production, conversion, and utilization of biofuels can help increase environmental and economic security locally and globally. Individual papers are processed separately for the data base.

  5. Indirect land use change and biofuel policy

    Science.gov (United States)

    Kocoloski, Matthew; Griffin, W. Michael; Matthews, H. Scott

    2009-09-01

    Biofuel debates often focus heavily on carbon emissions, with parties arguing for (or against) biofuels solely on the basis of whether the greenhouse gas emissions of biofuels are less than (or greater than) those of gasoline. Recent studies argue that land use change leads to significant greenhouse gas emissions, making some biofuels more carbon intensive than gasoline. We argue that evaluating the suitability and utility of biofuels or any alternative energy source within the limited framework of plus and minus carbon emissions is too narrow an approach. Biofuels have numerous impacts, and policy makers should seek compromises rather than relying solely on carbon emissions to determine policy. Here, we estimate that cellulosic ethanol, despite having potentially higher life cycle CO2 emissions (including from land use) than gasoline, would still be cost-effective at a CO2 price of 80 per ton or less, well above estimated CO2 mitigation costs for many alternatives. As an example of the broader approach to biofuel policy, we suggest the possibility of using the potential cost reductions of cellulosic ethanol relative to gasoline to balance out additional carbon emissions resulting from indirect land use change as an example of ways in which policies could be used to arrive at workable solutions.

  6. Global biofuel use, 1850-2000

    Science.gov (United States)

    Fernandes, Suneeta D.; Trautmann, Nina M.; Streets, David G.; Roden, Christoph A.; Bond, Tami C.

    2007-06-01

    This paper presents annual, country-level estimates of biofuel use for the period 1850-2000. We estimate that global biofuel consumption rose from about 1000 Tg in 1850 to 2460 Tg in 2000, an increase of 140%. In the late 19th century, biofuel consumption in North America was very high, ˜220-250 Tg/yr, because widespread land clearing supplied plentiful fuelwood. At that time biofuel use in Western Europe was lower, ˜180-200 Tg/yr. As fossil fuels became available, biofuel use in the developed world fell. Compensating changes in other parts of the world, however, caused global consumption to remain remarkably stable between 1850 and 1950 at ˜1200 ± 200 Tg/yr. It was only after World War II that biofuel use began to increase more rapidly in response to population growth in the developing world. Between 1950 and 2000, biofuel use in Africa, South Asia, and Southeast Asia grew by 170%, 160%, and 130%, respectively.

  7. Opportunity for profitable investments in cellulosic biofuels

    International Nuclear Information System (INIS)

    Babcock, Bruce A.; Marette, Stephan; Treguer, David

    2011-01-01

    Research efforts to allow large-scale conversion of cellulose into biofuels are being undertaken in the US and EU. These efforts are designed to increase logistic and conversion efficiencies, enhancing the economic competitiveness of cellulosic biofuels. However, not enough attention has been paid to the future market conditions for cellulosic biofuels, which will determine whether the necessary private investment will be available to allow a cellulosic biofuels industry to emerge. We examine the future market for cellulosic biofuels, differentiating between cellulosic ethanol and 'drop-in' cellulosic biofuels that can be transported with petroleum fuels and have equivalent energy values. We show that emergence of a cellulosic ethanol industry is unlikely without costly government subsidies, in part because of strong competition from conventional ethanol and limits on ethanol blending. If production costs of drop-in cellulosic biofuels fall enough to become competitive, then their expansion will not necessarily cause feedstock prices to rise. As long as local supplies of feedstocks that have no or low-valued alternative uses exist, then expansion will not cause prices to rise significantly. If cellulosic feedstocks come from dedicated biomass crops, then the supply curves will have a steeper slope because of competition for land. (author)

  8. External noise when using biofuel

    International Nuclear Information System (INIS)

    Kotaleski, J.

    1994-08-01

    The aim of this study has been to cover sources of noise dealing with all steps in a biofuel chain; producing, transporting, storing and firing the biofuel. When the availability of relevant test results from noise surveys is not so good and mostly badly documented, the study has been concentrated on estimation of external noise for planning and design purposes, from a prospective biofuel-fired plant. A synoptic tabulation of estimated acoustic power levels from different noise sources, has been done. The results from measurements of external noise from different existing combined power and heating plants are tabulated. The Nordic model for simulation of external noise has been used for a prospective plant - VEGA - designed by Vattenfall. The aim has been to estimate its noise pollutions at critical points at the nearest residential area (250 m from the fenced industry area). The software - ILYD - is easy to handle, but knowledge about the model is necessary. A requisite for the reliability is the access to measurements or estimations of different sources of noise, at different levels of octaves from 63 to 8000 Hz. The degree of accuracy increases with the number of broad band sources, that are integrated. Using ILYD with available data, a night limit of 40 dB(A) should be possible to fulfill with good degree of accuracy at VEGA, between 10 pm and 7 am, with good planning and under normal operation conditions. A demand for 35 dB(A) as a limit can be harder to fulfill, especially at mornings from 6 to 7. Noise from heavy vehicles within the plant area is classified as industrial noise and not as road traffic noise. This type of noise depends very much on the way of driving and assumed acceleration. Concerning wheel-mounted loaders, they may then only be used during daytime. The simulations show, that even at daytime from 7 to 6 pm, it would be possible to use an acoustically damped chipping machine, inside the power industry area. 31 refs, 13 figs, tabs, 8

  9. Metabolomics of Clostridial Biofuel Production

    Energy Technology Data Exchange (ETDEWEB)

    Rabinowitz, Joshua D [Princeton Univ., NJ (United States); Aristilde, Ludmilla [Cornell Univ., Ithaca, NY (United States); Amador-Noguez, Daniel [Univ. of Wisconsin, Madison, WI (United States)

    2015-09-08

    Members of the genus Clostridium collectively have the ideal set of the metabolic capabilities for fermentative biofuel production: cellulose degradation, hydrogen production, and solvent excretion. No single organism, however, can effectively convert cellulose into biofuels. Here we developed, using metabolomics and isotope tracers, basic science knowledge of Clostridial metabolism of utility for future efforts to engineer such an organism. In glucose fermentation carried out by the biofuel producer Clostridium acetobutylicum, we observed a remarkably ordered series of metabolite concentration changes as the fermentation progressed from acidogenesis to solventogenesis. In general, high-energy compounds decreased while low-energy species increased during solventogenesis. These changes in metabolite concentrations were accompanied by large changes in intracellular metabolic fluxes, with pyruvate directed towards acetyl-CoA and solvents instead of oxaloacetate and amino acids. Thus, the solventogenic transition involves global remodeling of metabolism to redirect resources from biomass production into solvent production. In contrast to C. acetobutylicum, which is an avid fermenter, C. cellulolyticum metabolizes glucose only slowly. We find that glycolytic intermediate concentrations are radically different from fast fermenting organisms. Associated thermodynamic and isotope tracer analysis revealed that the full glycolytic pathway in C. cellulolyticum is reversible. This arises from changes in cofactor utilization for phosphofructokinase and an alternative pathway from phosphoenolpyruvate to pyruvate. The net effect is to increase the high-energy phosphate bond yield of glycolysis by 150% (from 2 to 5) at the expense of lower net flux. Thus, C. cellulolyticum prioritizes glycolytic energy efficiency over speed. Degradation of cellulose results in other sugars in addition to glucose. Simultaneous feeding of stable isotope-labeled glucose and unlabeled pentose sugars

  10. Aptamer- and nucleic acid enzyme-based systems for simultaneous detection of multiple analytes

    Science.gov (United States)

    Lu, Yi [Champaign, IL; Liu, Juewen [Albuquerque, NM

    2011-11-15

    The present invention provides aptamer- and nucleic acid enzyme-based systems for simultaneously determining the presence and optionally the concentration of multiple analytes in a sample. Methods of utilizing the system and kits that include the sensor components are also provided. The system includes a first reactive polynucleotide that reacts to a first analyte; a second reactive polynucleotide that reacts to a second analyte; a third polynucleotide; a fourth polynucleotide; a first particle, coupled to the third polynucleotide; a second particle, coupled to the fourth polynucleotide; and at least one quencher, for quenching emissions of the first and second quantum dots, coupled to the first and second reactive polynucleotides. The first particle includes a quantum dot having a first emission wavelength. The second particle includes a second quantum dot having a second emission wavelength different from the first emission wavelength. The third polynucleotide and the fourth polynucleotide are different.

  11. Contrasts and synergies in different biofuel reports.

    Science.gov (United States)

    Michalopoulos, A; Landeweerd, L; Van der Werf-Kulichova, Z; Puylaert, P G B; Osseweijer, P

    2011-04-06

    The societal debate on biofuels is characterised by increased complexity. This can hinder the effective governance of the field. This paper attempts a quantitative bird's eye meta-analysis of this complexity by mapping different stakeholder perspectives and expected outcomes as seen in the secondary literature on biofuels, along the lines of the People-Planet-Profit framework. Our analysis illustrates the tension between stated and actual drivers of large scale biofuel development, especially for first generation biofuels. Although environmental (Planet) aspects have dominated the biofuel debate, their overall assessment is mostly negative with regard to first generation biofuels. By contrast, economic (Profit) aspects are the only ones that are assessed positively with regard to first generation biofuels. Furthermore, positive and negative assessments of biofuel development are strongly influenced by the differences in focus between different stakeholder clusters. Stakeholders who appear generally supportive to biofuel development (industry) focus relatively more on aspects that are generally assessed as positive (Profit). By contrast, non-supportive stakeholders (NGO's) tend to focus mainly on aspects that are generally assessed as negative (Planet). Moreover, our analysis of reference lists revealed few citations of primary scientific data, and also that intergovernmental organizations produce the most influential publications in the debate. The surprising lack of listed references to scientific (primary) data reveals a need to assess in which arena the transition of scientific data towards secondary publications takes place, and how one can measure its quality. This work should be understood as a first effort to take some control over a complex and contradictory number of publications, and to allow the effective governance of the field through the identification of areas of overlapping consensus and persisting controversy, without reverting to claims on

  12. Optical detection in microfluidic systems

    DEFF Research Database (Denmark)

    Mogensen, Klaus Bo; Kutter, Jörg Peter

    2009-01-01

    Optical detection schemes continue to be favoured for measurements in microfluidic systems. A selection of the latest progress mainly within the last two years is critically reviewed. Emphasis is on integrated solutions, such as planar waveguides, coupling schemes to the outside world, evanescent...... to ease commercialisation of the devices. This work will hopefully result in more commercial products that benefit from integrated optics, because the impact on commercial devices so far has been modest....

  13. Microfluidic Devices for Blood Fractionation

    OpenAIRE

    Hou, Han Wei; Bhagat, Ali Asgar S.; Lee, Wong Cheng J.; Huang, Sha; Han, Jongyoon; Lim, Chwee Teck

    2011-01-01

    Blood, a complex biological fluid, comprises 45% cellular components suspended in protein rich plasma. These different hematologic components perform distinct functions in vivo and thus the ability to efficiently fractionate blood into its individual components has innumerable applications in both clinical diagnosis and biological research. Yet, processing blood is not trivial. In the past decade, a flurry of new microfluidic based technologies has emerged to address this compelling problem. ...

  14. Bistable diverter valve in microfluidics

    Czech Academy of Sciences Publication Activity Database

    Tesař, Václav; Bandulasena, H.C.H.

    2011-01-01

    Roč. 50, č. 5 (2011), s. 1225-1233 ISSN 0723-4864 R&D Projects: GA ČR GA101/07/1499; GA AV ČR IAA200760705 Institutional research plan: CEZ:AV0Z20760514 Keywords : fluidics * bistable diverter valves * pressure-driven microfluidics Subject RIV: BK - Fluid Dynamics Impact factor: 1.735, year: 2011 http://www.springerlink.com/content/x4907p1908151522/

  15. Microfluidic Devices for Blood Fractionation

    Directory of Open Access Journals (Sweden)

    Chwee Teck Lim

    2011-07-01

    Full Text Available Blood, a complex biological fluid, comprises 45% cellular components suspended in protein rich plasma. These different hematologic components perform distinct functions in vivo and thus the ability to efficiently fractionate blood into its individual components has innumerable applications in both clinical diagnosis and biological research. Yet, processing blood is not trivial. In the past decade, a flurry of new microfluidic based technologies has emerged to address this compelling problem. Microfluidics is an attractive solution for this application leveraging its numerous advantages to process clinical blood samples. This paper reviews the various microfluidic approaches realized to successfully fractionate one or more blood components. Techniques to separate plasma from hematologic cellular components as well as isolating blood cells of interest including certain rare cells are discussed. Comparisons based on common separation metrics including efficiency (sensitivity, purity (selectivity, and throughput will be presented. Finally, we will provide insights into the challenges associated with blood-based separation systems towards realizing true point-of-care (POC devices and provide future perspectives.

  16. Liquid biofuels in the aeroderivative gas turbine

    Energy Technology Data Exchange (ETDEWEB)

    DiCampli, James; Schornick, Joe; Farr, Rachel

    2010-09-15

    While there are regional economic and political incentives for using liquid biofuels for renewable power generation, several challenges must be addressed. Given the fuel volumes required, base-load operation with renewable fuels such as biodiesel and ethanol are not likely sustainable with today's infrastructure. However, blending of biofuels with fossil fuels is a more economic option to provide renewable power. In turn, this lays the foundation to increase to more power generation in the future as new generation biofuels come on line. And, much like the automotive industry, the power industry will need to institute design changes to accommodate these fuels.

  17. Biofuels development and the policy regime.

    Science.gov (United States)

    Philp, Jim C; Guy, Ken; Ritchie, Rachael J

    2013-01-01

    Any major change to the energy order is certain to provoke both positive and negative societal responses. The current wave of biofuels development ignited controversies that have re-shaped the thinking about their future development. Mistakes were made in the early support for road transport biofuels in Organisation for Economic Co-operation and Development (OECD) countries. This article examines some of the policies that shaped the early development of biofuels and looks to the future. Copyright © 2012 Elsevier Ltd. All rights reserved.

  18. Biofuel Cells – Alternative Power Sources

    International Nuclear Information System (INIS)

    Babanova, Sofia; Yolina Hubenova; Mario Mitov

    2009-01-01

    Energy generation from renewable sources and effective waste treatment are two key challenges for the sustainable development. Microbiological (or Bio-) Fuel Cells provide an elegant solution by linking both tasks. Biofuel cells, which can directly generate electricity from biodegradable substances, have rapidly gained increasing research attention. Widely available fuel sources and moderate operational conditions make them promising in renewable energy generation, wastewater treatment, power sources for remote devices, etc. This paper reviews the use of microorganisms as biocatalysts in microbiological fuel cells. The principle of biofuel cells and their construction elements are discussed. Keywords: alternative power sources, biofuel cells, biocatalysts

  19. Session 8: biofuels; Session 8: Les biocarburants

    Energy Technology Data Exchange (ETDEWEB)

    Botte, J.M.

    2006-01-15

    Here are given the summaries of the speeches of Mr Daniel Le Breton (Total): the transports of the future: the role of biofuels; of Mr Pierre Rouveirolles (Renault): the future expectations and needs; of Mr Frederic Monot (IFP): the developments of new generations of biofuels from biomass; of Mr Willem Jan Laan (Unilever): the use of bio resources for food and fuel: a fair competition? All these speeches have been presented at the AFTP yearly days (12-13 october 2005) on the session 8 concerning the biofuels. (O.M.)

  20. Energy balance of solid biofuels

    International Nuclear Information System (INIS)

    Scholz, V.; Berg, W.; Kaulfuss, P.

    1998-01-01

    The input and output of energy are two important factors used to determine the energetic and ecological usefulness of a fuel or its production technology. In this paper, a number of different methods for the production of five biofuels which can be produced in agriculture and forestry are analysed and energetic balances are presented. The results show that the energetic input is relatively low compared to the output, especially for by-products and residual substances such as cereal straw and forest pruning timber (thinning). Whenever fuel crops are cultivated, the energetic efficiency is critically determined by the quantity of nitrogen applied. Depending on the crop and technology, each gigajoule of energy input can provide 7-30 GJ or with by-products up to 50 GJ of thermally utilizable energy without any additional CO 2 pollution. (author)

  1. Can the Nigerian biofuel policy and incentives (2007) transform Nigeria into a biofuel economy?

    International Nuclear Information System (INIS)

    Ohimain, Elijah I.

    2013-01-01

    Nigeria's economy is largely dependent on petroleum, yet the country is suffering from fuel supply shortages. In response to the transportation fuel supply difficulties in Nigeria, the country released the Nigerian Biofuel Policy and Incentives in 2007 to create favorable investment climate for the entrance of Nigeria into the biofuel sector. The paper assessed the progress made thus far by Nigeria, 4 years after the Nigerian biofuel was released in an attempt to answer the question whether the policy is adequate to transform Nigeria into a biofuel economy. The study found that little progress has been made, which includes commencement of the construction of 20 bioethanol factories, installation of biofuel handling facilities at two depots (Mosimi and Atlas Cove), and selection of retail outlets for biofuel/conventional fuel mix. The site construction of the announced biofuel projects is now slow and other progress is marginal. We therefore conclude that the Nigerian biofuel policy is unlikely to transform Nigeria into a biofuel economy unless the Government revert and refocus on biofuel and include additional financial incentives such as grants and subsidy to complement the tax waivers (income, import duty, VAT), loans, and insurance cover contained in the policy. - Highlights: ► Nigeria's economy is dependent on petroleum, yet the country is suffering from fuel shortages. ► The Nigerian Biofuel Policy and Incentives was released in 2007. ► Little progress has been made since the policy was released 4 years ago. ► Hence, the policy is unlikely to transform Nigeria into a biofuel economy

  2. Tracking U.S. biofuel innovation through patents

    International Nuclear Information System (INIS)

    Kessler, Jeff; Sperling, Daniel

    2016-01-01

    We use biofuel patents as a proxy for biofuel innovation. Through use of natural language processing and machine-learning algorithms, we expand patent classification capabilities to better explain the history of biofuels innovation. Results indicate that after the initial establishment of the U.S. biofuel industry, there were two surges in biofuel innovation: 1995–2000, characterized by heavy patenting by 1st generation (food-based) biofuel firms; and 2005–2010, characterized by a second surge of innovation by those same large firms, complemented by a large number of biotechnology firms producing a relatively small number of 2nd generation biofuel patents. Our analysis corroborates the widespread understanding that the first surge in biofuel innovation was linked to innovations in agriculture, and that the second surge of biofuel innovation was driven by demand-pull policies mandating and incentivizing biofuels. But the slow emergence of a 2nd generation cellulose-based biofuels industry, far slower than called for by policy, suggests that technology-push policies more focused on R&D and investment may be needed to accelerate the commercialization of 2nd generation biofuels. - Highlights: • Patenting activity closely corresponds to sociotechnical shifts in biofuel innovation. • The Renewable Fuel Standard likely contributed to the rise in biofuel patenting activity after 2005. • 2nd generation biofuel technology innovation appears lacking compared to 1st generation technologies.

  3. Materials for microfluidic chip fabrication.

    Science.gov (United States)

    Ren, Kangning; Zhou, Jianhua; Wu, Hongkai

    2013-11-19

    Through manipulating fluids using microfabricated channel and chamber structures, microfluidics is a powerful tool to realize high sensitive, high speed, high throughput, and low cost analysis. In addition, the method can establish a well-controlled microenivroment for manipulating fluids and particles. It also has rapid growing implementations in both sophisticated chemical/biological analysis and low-cost point-of-care assays. Some unique phenomena emerge at the micrometer scale. For example, reactions are completed in a shorter amount of time as the travel distances of mass and heat are relatively small; the flows are usually laminar; and the capillary effect becomes dominant owing to large surface-to-volume ratios. In the meantime, the surface properties of the device material are greatly amplified, which can lead to either unique functions or problems that we would not encounter at the macroscale. Also, each material inherently corresponds with specific microfabrication strategies and certain native properties of the device. Therefore, the material for making the device plays a dominating role in microfluidic technologies. In this Account, we address the evolution of materials used for fabricating microfluidic chips, and discuss the application-oriented pros and cons of different materials. This Account generally follows the order of the materials introduced to microfluidics. Glass and silicon, the first generation microfluidic device materials, are perfect for capillary electrophoresis and solvent-involved applications but expensive for microfabriaction. Elastomers enable low-cost rapid prototyping and high density integration of valves on chip, allowing complicated and parallel fluid manipulation and in-channel cell culture. Plastics, as competitive alternatives to elastomers, are also rapid and inexpensive to microfabricate. Their broad variety provides flexible choices for different needs. For example, some thermosets support in-situ fabrication of

  4. Figure 5, Biofuel refinery facility locations

    Data.gov (United States)

    U.S. Environmental Protection Agency — This workbook contains the locations and types of current and anticipated biofuel feedstock processing facilities assumed under the simulated scenarios. This dataset...

  5. Biofuel characteristics of beniseed (Sesanum indicum) oil

    African Journals Online (AJOL)

    SERVER

    2007-11-05

    Nov 5, 2007 ... Local method was used to extract oil from beniseed (Sesanum indicum). ... fuel properties similar to common biofuels, hence beniseed could be utilized as an .... industries for the manufacture of soap and vegetable oil –.

  6. IEA Energy Technology Essentials: Biofuel Production

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-01-15

    The IEA Energy Technology Essentials series offers concise four-page updates on the different technologies for producing, transporting and using energy. Biofuel Production is the topic covered in this edition.

  7. 3 CFR - Biofuels and Rural Economic Development

    Science.gov (United States)

    2010-01-01

    ... biofuels promise to play a key role by providing the Nation with homegrown sustainable energy options and... publish this memorandum in the Federal Register.BARACK OBAMATHE WHITE HOUSE, Washington, May 5, 2009. ...

  8. Technical solutions to make biofuels more competitive

    International Nuclear Information System (INIS)

    Anon.

    2006-01-01

    With the present day environmental and economical stakes, the French government has announced in 2005 a plan for the accelerated development of biofuels. In France, two traditional ways of biofuel generation exist: the bio-ethanol way and the bio-diesel way (methyl esters of vegetable oils). Two problems limit today the development of biofuels: the available cultivation surfaces and the production costs. The challenge of the next generation of biofuels concerns the better use of the available biomass, with no competition with the food productions, and in particular the development of ethyl esters of vegetable oils or the hydrogen processing of vegetable oils. Other processes are making their way, like the biomass to liquid (BTL) process, based on the Fischer-Tropsch synthesis, which allows to convert any type of biomass source into liquid fuels with a high production rate (about 5000 l/Ha). Short paper. (J.S.)

  9. Third Generation Biofuels via Direct Cellulose Fermentation

    Directory of Open Access Journals (Sweden)

    David B. Levin

    2008-07-01

    Full Text Available Consolidated bioprocessing (CBP is a system in which cellulase production, substrate hydrolysis, and fermentation are accomplished in a single process step by cellulolytic microorganisms. CBP offers the potential for lower biofuel production costs due to simpler feedstock processing, lower energy inputs, and higher conversion efficiencies than separate hydrolysis and fermentation processes, and is an economically attractive near-term goal for “third generation” biofuel production. In this review article, production of third generation biofuels from cellulosic feedstocks will be addressed in respect to the metabolism of cellulolytic bacteria and the development of strategies to increase biofuel yields through metabolic engineering.

  10. Biofuels and bioenergy: processes and technologies

    National Research Council Canada - National Science Library

    Lee, Sunggyu; Shah, Yatish T

    2013-01-01

    ... since the early twentieth century. Up until recently, however, development interest in biofuels had lessened due to the availability of relatively inexpensive fossil energy resources as well as the handling and transportation...

  11. setting sustainable standards for biofuel production

    African Journals Online (AJOL)

    OLAWUYI

    Director for Research, Training and International Development, Institute for Oil, Gas, ..... Table 3 presents the five stages in the product lifecycle for biofuel production ..... Principles on Human Rights Impact Assessments of Trade and Investment.

  12. Biofuels for transportation : a climate perspective

    Science.gov (United States)

    2008-06-01

    As the United States seeks to reduce greenhouse gas (GHG) emissions from motor vehicles and to lessen its dependence on imported oil, biofuels are gaining increasing attention as one possible solution. This paper offers an introduction to the current...

  13. Biofuels securing the planet's future energy needs

    International Nuclear Information System (INIS)

    Demirbas, Ayhan

    2009-01-01

    The biofuels include bioethanol, biobutanol, biodiesel, vegetable oils, biomethanol, pyrolysis oils, biogas, and biohydrogen. There are two global biomass based liquid transportation fuels that might replace gasoline and diesel fuel. These are bioethanol and biodiesel. World production of biofuel was about 68 billion L in 2007. The primary feedstocks of bioethanol are sugarcane and corn. Bioethanol is a gasoline additive/substitute. Bioethanol is by far the most widely used biofuel for transportation worldwide. About 60% of global bioethanol production comes from sugarcane and 40% from other crops. Biodiesel refers to a diesel-equivalent mono alkyl ester based oxygenated fuel. Biodiesel production using inedible vegetable oil, waste oil and grease has become more attractive recently. The economic performance of a biodiesel plant can be determined once certain factors are identified, such as plant capacity, process technology, raw material cost and chemical costs. The central policy of biofuel concerns job creation, greater efficiency in the general business environment, and protection of the environment.

  14. Detection methods for centrifugal microfluidic platforms

    DEFF Research Database (Denmark)

    Burger, Robert; Amato, Letizia; Boisen, Anja

    2016-01-01

    Centrifugal microfluidics has attracted much interest from academia as well as industry, since it potentially offers solutions for affordable, user-friendly and portable biosensing. A wide range of so-called fluidic unit operations, e.g. mixing, metering, liquid routing, and particle separation...... for the centrifugal microfluidics platform and cover optical as well as mechanical and electrical detection principles....

  15. Preface book Microfluidics for medical applications

    NARCIS (Netherlands)

    van den Berg, Albert; Segerink, Loes Irene

    2015-01-01

    This book presents an overview of the major microfluidics techniques and platforms used for medicine and medical applications, providing the reader with an overview of the recent developments in this field. It is divided in three parts: (1) tissue and organs on-chip, (2) microfluidics for medicine

  16. Cell Culture Microfluidic Biochips: Experimental Throughput Maximization

    DEFF Research Database (Denmark)

    Minhass, Wajid Hassan; Pop, Paul; Madsen, Jan

    2011-01-01

    Microfluidic biochips offer a promising alternative to a conventional biochemical laboratory, integrating all necessary functionalities on-chip in order to perform biochemical applications. Researchers have started to propose computer-aided design tools for the synthesis of such biochips. Our focus...... metaheuristic for experimental design generation for the cell culture microfluidic biochips, and we have evaluated our approach using multiple experimental setups....

  17. Modular microfluidic system for biological sample preparation

    Science.gov (United States)

    Rose, Klint A.; Mariella, Jr., Raymond P.; Bailey, Christopher G.; Ness, Kevin Dean

    2015-09-29

    A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis virus filter module, d) an isotachophoresis nucleic acid filter module, e) a lyses module, and f) an isotachophoresis-based nucleic acid filter.

  18. Principles, Techniques, and Applications of Tissue Microfluidics

    Science.gov (United States)

    Wade, Lawrence A.; Kartalov, Emil P.; Shibata, Darryl; Taylor, Clive

    2011-01-01

    The principle of tissue microfluidics and its resultant techniques has been applied to cell analysis. Building microfluidics to suit a particular tissue sample would allow the rapid, reliable, inexpensive, highly parallelized, selective extraction of chosen regions of tissue for purposes of further biochemical analysis. Furthermore, the applicability of the techniques ranges beyond the described pathology application. For example, they would also allow the posing and successful answering of new sets of questions in many areas of fundamental research. The proposed integration of microfluidic techniques and tissue slice samples is called "tissue microfluidics" because it molds the microfluidic architectures in accordance with each particular structure of each specific tissue sample. Thus, microfluidics can be built around the tissues, following the tissue structure, or alternatively, the microfluidics can be adapted to the specific geometry of particular tissues. By contrast, the traditional approach is that microfluidic devices are structured in accordance with engineering considerations, while the biological components in applied devices are forced to comply with these engineering presets.

  19. Opportunities for microfluidic technologies in synthetic biology

    OpenAIRE

    Gulati, Shelly; Rouilly, Vincent; Niu, Xize; Chappell, James; Kitney, Richard I.; Edel, Joshua B.; Freemont, Paul S.; deMello, Andrew J.

    2009-01-01

    We introduce microfluidics technologies as a key foundational technology for synthetic biology experimentation. Recent advances in the field of microfluidics are reviewed and the potential of such a technological platform to support the rapid development of synthetic biology solutions is discussed.

  20. Biofuels: The hidden cause of deforestation?

    OpenAIRE

    Smith, Alison; Lebensohn, Ignacio; Lickacz, Lindsay; Clarke, Louise

    2009-01-01

    The objective of the project is to establish a causal relationship between the biofuel market in the USA and the Amazonic Deforestation. The project parts from an objectivist approach and uses economic as well as environmental theories as a starting point. It attempts to demonstrate that biofuels are not as environmentally friendly as advertised, but instead have a detrimental effect on the Amazon Rainforest. The project utilizes statistics as a main source for empirical data, as well various...

  1. Panorama 2011: Water and bio-fuels

    International Nuclear Information System (INIS)

    Lorne, D.

    2011-01-01

    Nowadays, water is seen as a major sustainability criterion for bio-energies. Although the biofuels being produced by food crops are subject to the same risks as the farming sector as far as water resources are concerned, future sectors have a significant potential to reduce these risks, and this potential needs to be better understood in order for biofuels as a resource and their related technologies to develop properly. (authors)

  2. Applications of Microfluidics in Quantitative Biology.

    Science.gov (United States)

    Bai, Yang; Gao, Meng; Wen, Lingling; He, Caiyun; Chen, Yuan; Liu, Chenli; Fu, Xiongfei; Huang, Shuqiang

    2018-05-01

    Quantitative biology is dedicated to taking advantage of quantitative reasoning and advanced engineering technologies to make biology more predictable. Microfluidics, as an emerging technique, provides new approaches to precisely control fluidic conditions on small scales and collect data in high-throughput and quantitative manners. In this review, the authors present the relevant applications of microfluidics to quantitative biology based on two major categories (channel-based microfluidics and droplet-based microfluidics), and their typical features. We also envision some other microfluidic techniques that may not be employed in quantitative biology right now, but have great potential in the near future. © 2017 Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences. Biotechnology Journal Published by Wiley-VCH Verlag GmbH & Co. KGaA.

  3. Development of an Integrated Polymer Microfluidic Stack

    International Nuclear Information System (INIS)

    Datta, Proyag; Hammacher, Jens; Pease, Mark; Gurung, Sitanshu; Goettert, Jost

    2006-01-01

    Microfluidic is a field of considerable interest. While significant research has been carried out to develop microfluidic components, very little has been done to integrate the components into a complete working system. We present a flexible modular system platform that addresses the requirements of a complete microfluidic system. A microfluidic stack system is demonstrated with the layers of the stack being modular for specific functions. The stack and accompanying infrastructure provides an attractive platform for users to transition their design concepts into a working microfluidic system quickly with very little effort. The concept is demonstrated by using the system to carry out a chemilumiscence experiment. Details regarding the fabrication, assembly and experimental methods are presented

  4. Practical Packaging Technology for Microfluidic Systems

    International Nuclear Information System (INIS)

    Lee, Hwan Yong; Han, Song I; Han, Ki Ho

    2010-01-01

    This paper presents the technology for the design, fabrication, and characterization of a microfluidic system interface (MSI): the purpose of this technology is to enable the integration of complex microfluidic systems. The MSI technology can be applied in a simple manner for realizing complex arrangements of microfluidic interconnects, integrated microvalves for fluid control, and optical windows for on-chip optical processes. A microfluidic system for the preparation of genetic samples was used as the test vehicle to prove the effectiveness of the MSI technology for packaging complex microfluidic systems with multiple functionalities. The miniaturized genetic sample preparation system comprised several functional compartments, including compartments for cell purification, cell separation, cell lysis, solid-phase DNA extraction, polymerase chain reaction, and capillary electrophoresis. Additionally, the functional operation of the solid-phase extraction and PCR thermocycling compartments was demonstrated by using the MSI

  5. Manipulation of microfluidic droplets by electrorheological fluid

    KAUST Repository

    Zhang, Menying

    2009-09-01

    Microfluidics, especially droplet microfluidics, attracts more and more researchers from diverse fields, because it requires fewer materials and less time, produces less waste and has the potential of highly integrated and computer-controlled reaction processes for chemistry and biology. Electrorheological fluid, especially giant electrorheological fluid (GERF), which is considered as a kind of smart material, has been applied to the microfluidic systems to achieve active and precise control of fluid by electrical signal. In this review article, we will introduce recent results of microfluidic droplet manipulation, GERF and some pertinent achievements by introducing GERF into microfluidic system: digital generation, manipulation of "smart droplets" and droplet manipulation by GERF. Once it is combined with real-time detection, integrated chip with multiple functions can be realized. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

  6. An assessment of Thailand's biofuel development

    DEFF Research Database (Denmark)

    Kumar, S.; Salam, P. Abdul; Shrestha, Pujan

    2013-01-01

    The paper provides an assessment of first generation biofuel (ethanol and biodiesel) development in Thailand in terms of feedstock used, production trends, planned targets and policies and discusses the biofuel sustainability issues-environmental, socio-economic and food security aspects. The pol......The paper provides an assessment of first generation biofuel (ethanol and biodiesel) development in Thailand in terms of feedstock used, production trends, planned targets and policies and discusses the biofuel sustainability issues-environmental, socio-economic and food security aspects...... to land and water use and food security are important considerations to be addressed for its large scale application. Second generation biofuels derived from agricultural residues perform favorably on environmental and social sustainability issues in comparison to first generation biofuel sources...... as transportation fuel. Alternatively, the same amount of residue could provide 0.8-2.1 billion liters per year of diesel (biomass to Fischer-Tropsch diesel) to potentially offset 6%-15% of national diesel consumption in the transportation sector....

  7. Environmental and energy aspects of liquid biofuels

    International Nuclear Information System (INIS)

    De Boo, W.

    1993-02-01

    When spending public money to reduce CO 2 emissions, it is necessary to establish which alternative energy source results in the largest reduction of CO 2 emission per unit cost. Comparison of different biofuels with other energy resources is therefore important. Bioethanol is compared with leadfree gasoline, and rapeseed oil methylester (RME) is compared with diesel. Subsequently, biofuel production as a method to reduce CO 2 emission will be compared with other sustainable energy resources. This comparison is based on the energy balance in chapter two and the final costs of biofuels in chapter six. The comparison of biofuels and current fossil fuels is based on emissions to the atmosphere of greenhouse gases and acidifying pollutants in chapter three. Pollution to soil and water by arable cropping is a specific characteristic of biofuel production and is difficult to compare with fossil fuels. On this subject biofuels are compared with other land uses in chapter four. This also applies to other adverse environmental aspects of agricultural production such as competition for land use with natural areas and recreation purposes. To explore future technological developments, a comparison is made in energy balances with estimated results after the year 2000. The overall conclusion is that there are far better options to achieve CO 2 reduction. 2 figs., 9 tabs., 14 appendices, 28 refs

  8. Research Progress of Microfluidic Chips Preparation and its Optical Element

    Directory of Open Access Journals (Sweden)

    Feng WANG

    2014-03-01

    Full Text Available Microfluidic technology is the emerging technologies in researching fluid channel and related applications in the micro and nano-scale space. Microfluidic chip is a new miniaturized rapid analysis platform by microfluidic technology, it has many characteristics such as liquid flow control, minimal reagent consumption, rapid analysis, which is widely used in physics, chemistry, biology, and engineering science and other fields, it has strong interdisciplinary. This paper mainly discusses research progress of materials used for microfluidic chips and the devices based on microfluidic technology, including microfluidic chip, microfluidic optical devices, microfluidic laser preparation, microfluidic chip applications, focusing on the quasi-molecular laser processing technology and femtosecond laser processing technology in the microfluidic devices preparation, and make development prospects for it.

  9. Sustainability of biofuels in Latin America: Risks and opportunities

    Energy Technology Data Exchange (ETDEWEB)

    Janssen, Rainer, E-mail: rainer.janssen@wip-munich.de [WIP Renewable Energies, Sylvensteinstrasse 2, 81369 Munich (Germany); Rutz, Dominik Damian [WIP Renewable Energies, Sylvensteinstrasse 2, 81369 Munich (Germany)

    2011-10-15

    Several Latin American countries are setting up biofuel programmes to establish alternative markets for agricultural commodities. This is mainly triggered by the current success of Brazilian bioethanol production for the domestic market and for export. Furthermore, the global biofuel market is expected to increase due to ambitious biofuel programmes in the EU and in the USA. Colombia, Venezuela, Costa Rica and Guatemala are focusing on bioethanol production from sugarcane whereas biofuel production in Argentina is based on soy biodiesel. Recent developments of the biofuel sector take place extremely rapid especially in Argentina, which became one of the five largest biodiesel producers in the world in 2008. Till date no specific biofuel sustainability certification systems have been implemented in Latin American, as well as on global level. This fact and the predominant use of food crops for biofuel production raise concerns about the sustainability of biofuel production related to environmental and social aspects. This paper provides an overview of the hotspots of conflicts in biofuel production in Latin America. It investigates presently available sustainability tools and initiatives to ensure sustainable biofuel production in Latin America. Finally, it provides an outlook on how to integrate sustainability in the Latin American biofuel sector. - Research Highlights: > This study investigates risks and opportunities of biofuels in Latin America. > Latin American countries are setting up programmes to promote biofuel development. > Strong biofuel sectors provide opportunities for economic development. > Potential negative impact includes deforestation and effects on food security. > Sustainability initiatives exist to minimise negative impact.

  10. Sustainability of biofuels in Latin America: Risks and opportunities

    International Nuclear Information System (INIS)

    Janssen, Rainer; Rutz, Dominik Damian

    2011-01-01

    Several Latin American countries are setting up biofuel programmes to establish alternative markets for agricultural commodities. This is mainly triggered by the current success of Brazilian bioethanol production for the domestic market and for export. Furthermore, the global biofuel market is expected to increase due to ambitious biofuel programmes in the EU and in the USA. Colombia, Venezuela, Costa Rica and Guatemala are focusing on bioethanol production from sugarcane whereas biofuel production in Argentina is based on soy biodiesel. Recent developments of the biofuel sector take place extremely rapid especially in Argentina, which became one of the five largest biodiesel producers in the world in 2008. Till date no specific biofuel sustainability certification systems have been implemented in Latin American, as well as on global level. This fact and the predominant use of food crops for biofuel production raise concerns about the sustainability of biofuel production related to environmental and social aspects. This paper provides an overview of the hotspots of conflicts in biofuel production in Latin America. It investigates presently available sustainability tools and initiatives to ensure sustainable biofuel production in Latin America. Finally, it provides an outlook on how to integrate sustainability in the Latin American biofuel sector. - Research Highlights: → This study investigates risks and opportunities of biofuels in Latin America. → Latin American countries are setting up programmes to promote biofuel development. → Strong biofuel sectors provide opportunities for economic development. → Potential negative impact includes deforestation and effects on food security. → Sustainability initiatives exist to minimise negative impact.

  11. Optical calorimetry in microfluidic droplets.

    Science.gov (United States)

    Chamoun, Jacob; Pattekar, Ashish; Afshinmanesh, Farzaneh; Martini, Joerg; Recht, Michael I

    2018-05-29

    A novel microfluidic calorimeter that measures the enthalpy change of reactions occurring in 100 μm diameter aqueous droplets in fluoropolymer oil has been developed. The aqueous reactants flow into a microfluidic droplet generation chip in separate fluidic channels, limiting contact between the streams until immediately before they form the droplet. The diffusion-driven mixing of reactants is predominantly restricted to within the droplet. The temperature change in droplets due to the heat of reaction is measured optically by recording the reflectance spectra of encapsulated thermochromic liquid crystals (TLC) that are added to one of the reactant streams. As the droplets travel through the channel, the spectral characteristics of the TLC represent the internal temperature, allowing optical measurement with a precision of ≈6 mK. The microfluidic chip and all fluids are temperature controlled, and the reaction heat within droplets raises their temperature until thermal diffusion dissipates the heat into the surrounding oil and chip walls. Position resolved optical temperature measurement of the droplets allows calculation of the heat of reaction by analyzing the droplet temperature profile over time. Channel dimensions, droplet generation rate, droplet size, reactant stream flows and oil flow rate are carefully balanced to provide rapid diffusional mixing of reactants compared to thermal diffusion, while avoiding thermal "quenching" due to contact between the droplets and the chip walls. Compared to conventional microcalorimetry, which has been used in this work to provide reference measurements, this new continuous flow droplet calorimeter has the potential to perform titrations ≈1000-fold faster while using ≈400-fold less reactants per titration.

  12. Liquid biofuels - can they meet our expectations?

    Science.gov (United States)

    Glatzel, G.

    2012-04-01

    Liquid biofuels are one of the options for reducing the emission of greenhouse gases and the dependence on fossil fuels. This is reflected in the DIRECTIVE 2003/30/EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL on the promotion of the use of biofuels or other renewable fuels for transport. The promotion of E10, an automotive fuel containing 10 percent bioethanol, is based on this directive. At present almost all bioethanol is produced from agricultural crops such as maize, corn or sugar beet and sugar cane in suitable climates. In view of shortages and rising prices of food, in particular in developing countries, the use of food and feed crops for biofuel production is increasingly criticized. Alternative sources of biomass are perennial grasses and wood, whose cellulose fraction can be converted to alcohol by the so called "second generation" processes, which seem to be close to commercial deployment. The use of the total plant biomass increases the biofuel yield per hectare as compared to conventional crops. Of special interest for biofuel production is woody biomass from forests as this avoids competition with food production on arable land. Historically woody biomass was for millennia the predominant source of thermal energy. Before fossil fuels came into use, up to 80 percent of a forest was used for fuel wood, charcoal and raw materials such as potash for trade and industry. Now forests are managed to yield up to 80 percent of high grade timber for the wood industry. Replacing sophisticatedly managed forests by fast growing biofuel plantations could make economic sense for land owners when a protected market is guaranteed by politics, because biofuel plantations would be highly mechanized and cheap to operate, even if costs for certified planting material and fertilizer are added. For forest owners the decision to clear existing long rotation forests for biofuel plantations would still be weighty because of the extended time of decades required to rebuild a

  13. Enzyme-based solutions for textile processing and dye contaminant biodegradation-a review.

    Science.gov (United States)

    Chatha, Shahzad Ali Shahid; Asgher, Muhammad; Iqbal, Hafiz M N

    2017-06-01

    The textile industry, as recognized conformist and stake industry in the world's economy, is facing serious environmental challenges. In numerous industries, in practice, various chemical-based processes from initial sizing to final washing are fascinating harsh environment concerns. Some of these chemicals are corrosive to equipment and cause serious damage itself. Therefore, in the twenty-first century, chemical and allied industries quest a paradigm transition from traditional chemical-based concepts to a greener, sustainable, and environmentally friendlier catalytic alternative, both at the laboratory and industrial scales. Bio-based catalysis offers numerous benefits in the context of biotechnological industry and environmental applications. In recent years, bio-based processing has received particular interest among the scientist for inter- and multi-disciplinary investigations in the areas of natural and engineering sciences for the application in biotechnology sector at large and textile industries in particular. Different enzymatic processes such as chemical substitution have been developed or in the process of development for various textile wet processes. In this context, the present review article summarizes current developments and highlights those areas where environment-friendly enzymatic textile processing might play an increasingly important role in the textile industry. In the first part of the review, a special focus has been given to a comparative discussion of the chemical-based "classical/conventional" treatments and the modern enzyme-based treatment processes. Some relevant information is also reported to identify the major research gaps to be worked out in future.

  14. Parsing glucose entry into the brain: novel findings obtained with enzyme-based glucose biosensors.

    Science.gov (United States)

    Kiyatkin, Eugene A; Wakabayashi, Ken T

    2015-01-21

    Extracellular levels of glucose in brain tissue reflect dynamic balance between its gradient-dependent entry from arterial blood and its use for cellular metabolism. In this work, we present several sets of previously published and unpublished data obtained by using enzyme-based glucose biosensors coupled with constant-potential high-speed amperometry in freely moving rats. First, we consider basic methodological issues related to the reliability of electrochemical measurements of extracellular glucose levels in rats under physiologically relevant conditions. Second, we present data on glucose responses induced in the nucleus accumbens (NAc) by salient environmental stimuli and discuss the relationships between local neuronal activation and rapid glucose entry into brain tissue. Third, by presenting data on changes in NAc glucose induced by intravenous and intragastric glucose delivery, we discuss other mechanisms of glucose entry into the extracellular domain following changes in glucose blood concentrations. Lastly, by showing the pattern of NAc glucose fluctuations during glucose-drinking behavior, we discuss the relationships between "active" and "passive" glucose entry to the brain, its connection to behavior-related metabolic activation, and the possible functional significance of these changes in behavioral regulation. These data provide solid experimental support for the "neuronal" hypothesis of neurovascular coupling, which postulates the critical role of neuronal activity in rapid regulation of vascular tone, local blood flow, and entry of glucose and oxygen to brain tissue to maintain active cellular metabolism.

  15. Amperometric Enzyme-based Gas Sensor for Formaldehyde: Impact of Possible Interferences

    Directory of Open Access Journals (Sweden)

    Ralf Moos

    2007-02-01

    Full Text Available In this work, cross-sensitivities and environmental influences on the sensitivityand the functionality of an enzyme-based amperometric sensor system for the directdetection of formaldehyde from the gas phase are studied. The sensor shows a linearresponse curve for formaldehyde in the tested range (0 - 15 vppm with a sensitivity of1.9 μA/ppm and a detection limit of about 130 ppb. Cross-sensitivities by environmentalgases like CO2, CO, NO, H2, and vapors of organic solvents like methanol and ethanol areevaluated as well as temperature and humidity influences on the sensor system. The sensorshowed neither significant signal to CO, H2, methanol or ethanol nor to variations in thehumidity of the test gas. As expected, temperature variations had the biggest influence onthe sensor sensitivity with variations in the sensor signal of up to 10 % of the signal for 5vppm CH2O in the range of 25 - 30 °C.

  16. Functional Enzyme-Based Approach for Linking Microbial Community Functions with Biogeochemical Process Kinetics

    Energy Technology Data Exchange (ETDEWEB)

    Li, Minjing [School; Qian, Wei-jun [Pacific Northwest National Laboratory, Richland, Washington 99354, United States; Gao, Yuqian [Pacific Northwest National Laboratory, Richland, Washington 99354, United States; Shi, Liang [School; Liu, Chongxuan [Pacific Northwest National Laboratory, Richland, Washington 99354, United States; School

    2017-09-28

    The kinetics of biogeochemical processes in natural and engineered environmental systems are typically described using Monod-type or modified Monod-type models. These models rely on biomass as surrogates for functional enzymes in microbial community that catalyze biogeochemical reactions. A major challenge to apply such models is the difficulty to quantitatively measure functional biomass for constraining and validating the models. On the other hand, omics-based approaches have been increasingly used to characterize microbial community structure, functions, and metabolites. Here we proposed an enzyme-based model that can incorporate omics-data to link microbial community functions with biogeochemical process kinetics. The model treats enzymes as time-variable catalysts for biogeochemical reactions and applies biogeochemical reaction network to incorporate intermediate metabolites. The sequences of genes and proteins from metagenomes, as well as those from the UniProt database, were used for targeted enzyme quantification and to provide insights into the dynamic linkage among functional genes, enzymes, and metabolites that are necessary to be incorporated in the model. The application of the model was demonstrated using denitrification as an example by comparing model-simulated with measured functional enzymes, genes, denitrification substrates and intermediates

  17. Adapting enzyme-based microbial water quality analysis to remote areas in low-income countries.

    Science.gov (United States)

    Abramson, Adam; Benami, Maya; Weisbrod, Noam

    2013-09-17

    Enzyme-substrate microbial water tests, originally developed for efficiency gains in laboratory settings, are potentially useful for on-site analysis in remote settings. This is especially relevant in developing countries where water quality is a pressing concern and qualified laboratories are rare. We investigated one such method, Colisure, first for sensitivity to incubation temperatures in order to explore alternative incubation techniques appropriate for remote areas, and then in a remote community of Zambia for detection of total coliforms and Escherichia coli in drinking-water samples. We sampled and analyzed 352 water samples from source, transport containers and point-of-use from 164 random households. Both internal validity (96-100%) and laboratory trials (zero false negatives or positives at incubation between 30 and 40 °C) established reliability under field conditions. We therefore recommend the use of this and other enzyme-based methods for remote applications. We also found that most water samples from wells accessing groundwater were free of E. coli whereas most samples from surface sources were fecally contaminated. We further found very low awareness among the population of the high levels of recontamination in household storage containers, suggesting the need for monitoring and treatment beyond the water source itself.

  18. Amperometric Enzyme-Based Biosensors for Application in Food and Beverage Industry

    Science.gov (United States)

    Csöoregi, Elisabeth; Gáspñr, Szilveszter; Niculescu, Mihaela; Mattiasson, Bo; Schuhmann, Wolfgang

    Continuous, sensitive, selective, and reliable monitoring of a large variety of different compounds in various food and beverage samples is of increasing importance to assure a high-quality and tracing of any possible source of contamination of food and beverages. Most of the presently used classical analytical methods are often requiring expensive instrumentation, long analysis times and well-trained staff. Amperometric enzyme-based biosensors on the other hand have emerged in the last decade from basic science to useful tools with very promising application possibilities in food and beverage industry. Amperometric biosensors are in general highly selective, sensitive, relatively cheap, and easy to integrate into continuous analysis systems. A successful application of such sensors for industrial purposes, however, requires a sensor design, which satisfies the specific needs of monitoring the targeted analyte in the particular application, Since each individual application needs different operational conditions and sensor characteristics, it is obvious that biosensors have to be tailored for the particular case. The characteristics of the biosensors are depending on the used biorecognition element (enzyme), nature of signal transducer (electrode material) and the communication between these two elements (electron-transfer pathway).

  19. Privileged Biofuels, Marginalized Indigenous Peoples: The Coevolution of Biofuels Development in the Tropics

    Science.gov (United States)

    Montefrio, Marvin Joseph F.

    2012-01-01

    Biofuels development has assumed an important role in integrating Indigenous peoples and other marginalized populations in the production of biofuels for global consumption. By combining the theories of commoditization and the environmental sociology of networks and flows, the author analyzed emerging trends and possible changes in institutions…

  20. Biofuels Fuels Technology Pathway Options for Advanced Drop-in Biofuels Production

    Energy Technology Data Exchange (ETDEWEB)

    Kevin L Kenney

    2011-09-01

    Advanced drop-in hydrocarbon biofuels require biofuel alternatives for refinery products other than gasoline. Candidate biofuels must have performance characteristics equivalent to conventional petroleum-based fuels. The technology pathways for biofuel alternatives also must be plausible, sustainable (e.g., positive energy balance, environmentally benign, etc.), and demonstrate a reasonable pathway to economic viability and end-user affordability. Viable biofuels technology pathways must address feedstock production and environmental issues through to the fuel or chemical end products. Potential end products include compatible replacement fuel products (e.g., gasoline, diesel, and JP8 and JP5 jet fuel) and other petroleum products or chemicals typically produced from a barrel of crude. Considering the complexity and technology diversity of a complete biofuels supply chain, no single entity or technology provider is capable of addressing in depth all aspects of any given pathway; however, all the necessary expert entities exist. As such, we propose the assembly of a team capable of conducting an in-depth technology pathway options analysis (including sustainability indicators and complete LCA) to identify and define the domestic biofuel pathways for a Green Fleet. This team is not only capable of conducting in-depth analyses on technology pathways, but collectively they are able to trouble shoot and/or engineer solutions that would give industrial technology providers the highest potential for success. Such a team would provide the greatest possible down-side protection for high-risk advanced drop-in biofuels procurement(s).

  1. Magnetic separation in microfluidic systems

    DEFF Research Database (Denmark)

    Smistrup, Kristian

    2007-01-01

    to facilitate real-time monitoring of the experiments. The set-up and experimental protocol are described in detail. Results are presented for ’active’ magnetic bead separators, where on-chip microfabricated electromagnets supply the magnetic field and field gradients necessary for magnetic bead separation....... It is shown conceptually how such a system can be applied for parallel biochemical processing in a microfluidic system. ’Passive’ magnetic separators are presented, where on-chip soft magnetic elements are magnetized by an external magnetic field and create strong magnetic fields and gradients inside...

  2. Microfluidics and microscale transport processes

    CERN Document Server

    Chakraborty, Suman

    2012-01-01

    With an intense focus on micro- and nanotechnology from a fluidic perspective, this book details the research activities in key directions on both the theoretical and experimental fronts. As part of the IIT Kharagpur Research Monograph series, the text discusses topics such as capillary transport in microchannels, fluid friction and heat transfer in microchannels, electrokinetics, and interfacial transport in nanochannels. It also covers nanoparticle transport in colloidal suspensions, bubble generation in microfluidic channels, micro-heat pipe, the lattice Boltzmann method for phase changing

  3. Microfluidic Approach to Cell Microencapsulation.

    Science.gov (United States)

    Sharma, Varna; Hunckler, Michael; Ramasubramanian, Melur K; Opara, Emmanuel C; Katuri, Kalyan C

    2017-01-01

    Bioartificial pancreas made of insulin-secreting islets cells holds great promise in the treatment of individuals with Type-1 diabetes. Successful islet cell microencapsulation in biopolymers is a key step for providing immunoisolation of transplanted islet cells. Because of the variability in the size and shape of pancreatic islets, one of the main obstacles in their microencapsulation is the inability to consistently control shape, size, and microstructure of the encapsulating biopolymer capsule. In this chapter, we provide a detailed description of a microfluidic approach to islet cell encapsulation in alginate that might address the microencapsulation challenges.

  4. Flambeau River Biofuels Demonstration Plant

    Energy Technology Data Exchange (ETDEWEB)

    Byrne, Robert J. [Flambeau River Biofuels, Inc., Park Falls, WI (United States)

    2012-07-30

    Flambeau River BioFuels, Inc. (FRB) proposed to construct a demonstration biomass-to-liquids (BTL) biorefinery in Park Falls, Wisconsin. The biorefinery was to be co-located at the existing pulp and paper mill, Flambeau River Papers, and when in full operation would both generate renewable energy – making Flambeau River Papers the first pulp and paper mill in North America to be nearly fossil fuel free – and produce liquid fuels from abundant and renewable lignocellulosic biomass. The biorefinery would serve to validate the thermochemical pathway and economic models for BTL production using forest residuals and wood waste, providing a basis for proliferating BTL conversion technologies throughout the United States. It was a project goal to create a compelling new business model for the pulp and paper industry, and support the nation’s goal for increasing renewable fuels production and reducing its dependence on foreign oil. FRB planned to replicate this facility at other paper mills after this first demonstration scale plant was operational and had proven technical and economic feasibility.

  5. Biofuels and the biorefinery concept

    International Nuclear Information System (INIS)

    Taylor, Gail

    2008-01-01

    Liquid fuels can be made by refining a range of biomass materials, including oil-rich and sugar-rich crops such as oil-seed rape and sugar beet, biomass that consists mainly of plant cell walls (second generation lignocellulosics), macro- and micro-alga, or material that would now be discarded as waste. This can include animal bi-products as well as waste wood and other resources. In the medium-term, plant cell (lignocellulosic) material is likely to be favoured as the feedstock for biorefineries because of its availability. The UK may make use of a number of these options because of its complex agricultural landscape. There are now a range of targets for biofuel use in the UK, although their environmental effects are disputed. The technology of refining these materials is well known. Possible outputs include biodiesel and bioethanol, both of which can be used as transport fuel. Other potential products include hydrogen, polymers and a wide range of value-added chemicals, making this technology important in a post-petrochemical world. Biorefineries could use cogeneration to produce electricity. The paper identifies a range of research and development priorities which must be met if this opportunity is to be exploited fully

  6. Self-contained microfluidic systems: a review.

    Science.gov (United States)

    Boyd-Moss, Mitchell; Baratchi, Sara; Di Venere, Martina; Khoshmanesh, Khashayar

    2016-08-16

    Microfluidic systems enable rapid diagnosis, screening and monitoring of diseases and health conditions using small amounts of biological samples and reagents. Despite these remarkable features, conventional microfluidic systems rely on bulky expensive external equipment, which hinders their utility as powerful analysis tools outside of research laboratories. 'Self-contained' microfluidic systems, which contain all necessary components to facilitate a complete assay, have been developed to address this limitation. In this review, we provide an in-depth overview of self-contained microfluidic systems. We categorise these systems based on their operating mechanisms into three major groups: passive, hand-powered and active. Several examples are provided to discuss the structure, capabilities and shortcomings of each group. In particular, we discuss the self-contained microfluidic systems enabled by active mechanisms, due to their unique capability for running multi-step and highly controllable diagnostic assays. Integration of self-contained microfluidic systems with the image acquisition and processing capabilities of smartphones, especially those equipped with accessory optical components, enables highly sensitive and quantitative assays, which are discussed. Finally, the future trends and possible solutions to expand the versatility of self-contained, stand-alone microfluidic platforms are outlined.

  7. Microfluidic cell culture systems for drug research.

    Science.gov (United States)

    Wu, Min-Hsien; Huang, Song-Bin; Lee, Gwo-Bin

    2010-04-21

    In pharmaceutical research, an adequate cell-based assay scheme to efficiently screen and to validate potential drug candidates in the initial stage of drug discovery is crucial. In order to better predict the clinical response to drug compounds, a cell culture model that is faithful to in vivo behavior is required. With the recent advances in microfluidic technology, the utilization of a microfluidic-based cell culture has several advantages, making it a promising alternative to the conventional cell culture methods. This review starts with a comprehensive discussion on the general process for drug discovery and development, the role of cell culture in drug research, and the characteristics of the cell culture formats commonly used in current microfluidic-based, cell-culture practices. Due to the significant differences in several physical phenomena between microscale and macroscale devices, microfluidic technology provides unique functionality, which is not previously possible by using traditional techniques. In a subsequent section, the niches for using microfluidic-based cell culture systems for drug research are discussed. Moreover, some critical issues such as cell immobilization, medium pumping or gradient generation in microfluidic-based, cell-culture systems are also reviewed. Finally, some practical applications of microfluidic-based, cell-culture systems in drug research particularly those pertaining to drug toxicity testing and those with a high-throughput capability are highlighted.

  8. In vivo continuous and simultaneous monitoring of brain energy substrates with a multiplex amperometric enzyme-based biosensor device

    NARCIS (Netherlands)

    De Lima Braga Lopes Cordeiro, Carlos; de Vries, M.G.; Ngabi, W; Oomen, P.E.; Cremers, T.I.F.H.; Westerink, B.H.C.

    2015-01-01

    Enzyme-based amperometric biosensors are widely used for monitoring key biomarkers. In experimental neuroscience there is a growing interest in in vivo continuous and simultaneous monitoring of metabolism-related biomarkers, like glucose, lactate and pyruvate. The use of multiplex biosensors will

  9. Allies in Biofuels. Opportunities in the Dutch - Argentinean biofuels trade relation

    International Nuclear Information System (INIS)

    Verhagen, M.

    2007-01-01

    First generation biofuels as an environmental solution are showing their own negative environmental, social and economic side effects. These need to be dealt with, because it is apparent that those same biofuels can be produced in a sustainable manner, thereby contributing to a healthier planet. Since both Argentina and the Netherlands would benefit from sustainable biofuels trade, policy measures need to be taken to guide the proper way. In what manner could bilateral cooperation concerning biofuels, optimize trade and policy output in both countries? By answering this question, one can hand solutions to upcoming problems - barriers to a sustainable energy structure - while at the same time facilitating trade between Argentina and the Netherlands. Besides providing information about the European, Dutch and Argentine market, this report presents an overview of biofuel policies. Special attention is given to the issue of sustainable biofuel production, in order to spread the necessary awareness, create wide support for corresponding politics, and offer opportunities for cooperation to prevent future entrapment. An entrapment, which could easily occur when actors in politics and business ignore international requirements for sustainable biofuel production. The research aims to produce the following output: Policy recommendations regarding the promotion of environmentally sound biofuels in both countries; A set arena to support a policy dialogue between both countries; An overview of current Dutch and Argentinean biofuel policies; Up to date information on current volumes of production, consumption and trade; Data with contact information of partners in both countries. Argentina shows an extremely professional agricultural sector, producing large quantities of vegetable oils, specifically of soybean. This sector has started to turn its attention towards biofuels - particularly to biodiesel. Projected production (for 2007-2008) is astonishingly high. The sector mainly

  10. Fluorescence detection system for microfluidic droplets

    Science.gov (United States)

    Chen, Binyu; Han, Xiaoming; Su, Zhen; Liu, Quanjun

    2018-05-01

    In microfluidic detection technology, because of the universality of optical methods in laboratory, optical detection is an attractive solution for microfluidic chip laboratory equipment. In addition, the equipment with high stability and low cost can be realized by integrating appropriate optical detection technology on the chip. This paper reports a detection system for microfluidic droplets. Photomultiplier tubes (PMT) is used as a detection device to improve the sensitivity of detection. This system improves the signal to noise ratio by software filtering and spatial filter. The fluorescence intensity is proportional to the concentration of the fluorescence and intensity of the laser. The fluorescence micro droplets of different concentrations can be distinguished by this system.

  11. Synthetic biology and the technicity of biofuels.

    Science.gov (United States)

    Mackenzie, Adrian

    2013-06-01

    The principal existing real-world application of synthetic biology is biofuels. Several 'next generation biofuel' companies-Synthetic Genomics, Amyris and Joule Unlimited Technologies-claim to be using synthetic biology to make biofuels. The irony of this is that highly advanced science and engineering serves the very mundane and familiar realm of transport. Despite their rather prosaic nature, biofuels could offer an interesting way to highlight the novelty of synthetic biology from several angles at once. Drawing on the French philosopher of technology and biology Gilbert Simondon, we can understand biofuels as technical objects whose genesis involves processes of concretisation that negotiate between heterogeneous geographical, biological, technical, scientific and commercial realities. Simondon's notion of technicity, the degree of concretisation of a technical object, usefully conceptualises this relationality. Viewed in terms of technicity, we might understand better how technical entities, elements, and ensembles are coming into being in the name of synthetic biology. The broader argument here is that when we seek to identify the newness of disciplines, their newness might be less epistemic and more logistic. Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.

  12. Perspectives for Sustainable Aviation Biofuels in Brazil

    Directory of Open Access Journals (Sweden)

    Luís A. B. Cortez

    2015-01-01

    Full Text Available The aviation industry has set ambitious goals to reduce carbon emissions in coming decades. The strategy involves the use of sustainable biofuels, aiming to achieve benefits from environmental, social, and economic perspectives. In this context, Brazilian conditions are favorable, with a mature agroindustry that regularly produces automotive biofuel largely adopted by Brazilian road vehicles, while air transportation has been growing at an accelerating pace and a modern aircraft industry is in place. This paper presents the main conclusions and recommendations from a broad assessment of the technological, economic, and sustainability challenges and opportunities associated with the development of drop-in aviation biofuels in Brazil. It was written by a research team that prepared the initial reports and conducted eight workshops with the active participation of more than 30 stakeholders encompassing the private sector, government institutions, NGOs, and academia. The main outcome was a set of guidelines for establishing a new biofuels industry, including recommendations for (a filling the identified research and development knowledge gaps in the production of sustainable feedstock; (b overcoming the barriers in conversion technology, including scaling-up issues; (c promoting greater involvement and interaction between private and government stakeholders; and (d creating a national strategy to promote the development of aviation biofuels.

  13. Beyond commonplace biofuels: Social aspects of ethanol

    International Nuclear Information System (INIS)

    Ribeiro, Barbara Esteves

    2013-01-01

    Biofuels policies and projects may lead to environmental, economic and social impacts. A number of studies point out the need to deliver comprehensive sustainability assessments regarding biofuels, with some presenting analytical frameworks that claim to be exhaustive. However, what is often found in the literature is an overexploitation of environmental and economic concerns, by contrast to a limited appraisal of the social aspects of biofuels. Building on a systematic review of the peer-reviewed literature, this paper discusses the social constraints and strengths of ethanol, with regard to the product's lifecycle stages and the actors involved. Its objective is to contribute to the development of social frameworks to be used in assessing the impact of ethanol. Main findings indicate that ethanol developments can increase the levels of social vulnerability, although there is little evidence in the literature regarding the positive and negative social impacts of 1st-generation ethanol and potential impacts of cellulosic ethanol. Further work is needed on the formulation of social criteria and indicators for a comprehensive sustainability assessment of this biofuel. Policy makers need to internalise the social dimension of ethanol in decision-making to prevent public opposition and irreversible social costs in the future. - Highlights: ► The literature lacks evidence on the social impacts of ethanol. ► Further work is needed on social criteria and indicators for assessment. ► Ethanol developments can increase the levels of social vulnerability. ► Decision-making should internalise the social dimension of biofuels sustainability

  14. Round table on bio-fuels

    International Nuclear Information System (INIS)

    2005-11-01

    The French ministers of agriculture and of industry have organized a meeting with the main French actors of agriculture, petroleum industry, car making and accessories industry and with professionals of agriculture machines to encourage the development of bio-fuels in France. This meeting took place in Paris in November 21, 2005. Its aim was to favor the partnerships between the different actors and the public authorities in order to reach the ambitious goals of the government of 5.75% of bio-fuels in fossil fuels by 2008, 7% by 2010 and 10% by 2015. The main points discussed by the participants were: the compatibility of automotive fuel standards with the objectives of bio-fuel incorporation, the development of direct incorporation of methanol in gasoline, the ethanol-ETBE partnership, the question of the lower calorific value of ETBE (ethyl tertio butyl ether), the development of new bio-fuels, the development of bio-diesel and the specific case of pure vegetal oils, and the fiscal framework of bio-fuels. This meeting has permitted to reach important improvements with 15 concrete agreements undertaken by the participants. (J.S.)

  15. Bio-fuel production potential in Romania

    International Nuclear Information System (INIS)

    Laurentiu, F.; Silvian, F.; Dumitru, F.

    2006-01-01

    The paper is based on the ESTO Study: Techno- Economic Feasibility of Large-Scale Production of Bio-Fuels in EU-Candidate Countries. Bio-fuel production has not been taken into account significantly until now in Romania, being limited to small- scale productions of ethanol, used mostly for various industrial purposes. However the climatic conditions and the quality of the soil are very suitable in the country for development of the main crops (wheat, sugar-beet, sunflower and rape-seed) used in bio-ethanol and bio-diesel production. The paper intended to consider a pertinent discussion of the present situation in Romania's agriculture stressing on the following essential items in the estimation of bio-fuels production potential: availability of feed-stock for bio-fuel production; actual productions of bio-fuels; fuel consumption; cost assessment; SWOT approach; expected trends. Our analysis was based on specific agricultural data for the period 1996-2000. An important ethanol potential (due to wheat, sugar-beet and maize cultures), as well as bio-diesel one (due to sun-flower and rape-seed) were predicted for the period 2005-2010 which could be exploited with the support of an important financial and technological effort, mainly from EU countries

  16. Forecast for biofuel trade in Europe

    International Nuclear Information System (INIS)

    Hektor, B.; Vinterbaeck, J.; Toro, A.de; Nilsson, Daniel

    1993-01-01

    One principal general conclusion is that the European biofuel market for the period up to the year 2000 will be competitive, dynamic and affected by technical development and innovations. That leads to the conclusion that prices will go down, which will increase the ability of biofuels to compete in the market. Still, biofuels will generally not be able to compete at the price level of fossil fuels in the world market, but will need support or protection to reach a competitive position. There are several reasons for support, e.g. offsetting the green-house effect and acid rain, conservation of the limited fossil fuel deposits, utilisation of local and domestic energy resources, etc. As energy crops in Europe are at an introductory stage, no large international trade can be expected within the next ten years. In this study it is assumed that some limited protective measures are imposed, which is a possible result of the energy and environmental policy currently discussed for the European Community, EC. The study implies that in the year 2000 it is possible to transport large quantities of biofuels to large energy consumers if taxes and other incentives now under discussion in the EC and national governments are introduced. The study also implies that in the year 2000 it is possible to utilise biofuels primarily in local and national markets. In the latter case, international trade will be reduced to minor spot quantities

  17. Dynamic relationships between microbial biomass, respiration, inorganic nutrients and enzyme activities: informing enzyme based decomposition models

    Directory of Open Access Journals (Sweden)

    Daryl L Moorhead

    2013-08-01

    Full Text Available We re-examined data from a recent litter decay study to determine if additional insights could be gained to inform decomposition modeling. Rinkes et al. (2013 conducted 14-day laboratory incubations of sugar maple (Acer saccharum or white oak (Quercus alba leaves, mixed with sand (0.4% organic C content or loam (4.1% organic C. They measured microbial biomass C, carbon dioxide efflux, soil ammonium, nitrate, and phosphate concentrations, and β-glucosidase (BG, β-N-acetyl-glucosaminidase (NAG, and acid phosphatase (AP activities on days 1, 3, and 14. Analyses of relationships among variables yielded different insights than original analyses of individual variables. For example, although respiration rates per g soil were higher for loam than sand, rates per g soil C were actually higher for sand than loam, and rates per g microbial C showed little difference between treatments. Microbial biomass C peaked on day 3 when biomass-specific activities of enzymes were lowest, suggesting uptake of litter C without extracellular hydrolysis. This result refuted a common model assumption that all enzyme production is constitutive and thus proportional to biomass, and/or indicated that part of litter decay is independent of enzyme activity. The length and angle of vectors defined by ratios of enzyme activities (BG/NAG versus BG/AP represent relative microbial investments in C (length, and N and P (angle acquiring enzymes. Shorter lengths on day 3 suggested low C limitation, whereas greater lengths on day 14 suggested an increase in C limitation with decay. The soils and litter in this study generally had stronger P limitation (angles > 45˚. Reductions in vector angles to < 45˚ for sand by day 14 suggested a shift to N limitation. These relational variables inform enzyme-based models, and are usually much less ambiguous when obtained from a single study in which measurements were made on the same samples than when extrapolated from separate studies.

  18. Characterization of ashes from biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Frandsen, F.J.; Hansen, L.A. [Technical Univ. of Denmark. Dept. of Chemical Engineering (Denmark); Soerensen, H.S. [Geological Survey of Denmark and Greenland (Denmark); Hjuler, K. [dk-TEKNIK. Energy and Environment (Denmark)

    1998-02-01

    One motivation for initiating the present project was that the international standard method of estimating the deposit propensity of solid fuels, of which a number of variants exist (e.g. ISO, ASTM, SD, DIN), has shown to be unsuitable for biomass ashes. This goal was addressed by the development of two new methods for the detection of ash fusibility behaviour based on Simultaneous Thermal Analysis (STA) and High Temperature Light Microscopy (HTLM), respectively. The methods were developed specifically for ashes from biofuels, but are suitable for coal ashes as well. They have been tested using simple salt mixtures, geological standards and samples from straw CHP and coal-straw PF combustion plants. All samples were run in a nitrogen atmosphere at a heating rate of 10 deg. C/min. In comparison with the standard method, the new methods are objective and have superior repeatability and sensitivity. Furthermore, the two methods enable the melting behavior to be characterized by a continuous measurement of melt fraction versus temperature. Due to this two-dimensional resolution of the results, the STA and HTLM methods provide more information than the standard method. The study of bottom ash and fly ash as well as deposit samples from straw test firings at the Haslev and Slagelse Combined Heat and Power plants resulted in a better understanding of mineral behaviour during straw grate firing. In these tests a number of straws were fired which had been carefully selected for having different qualities with respect to sort and potassium and chlorine contents. By studying bottom ashes from Slagelse it was found that the melting behaviour correlated with the deposition rate on a probe situated at the outlet part of the combustion zone. (EG)

  19. Competitive liquid biofuels from biomass

    International Nuclear Information System (INIS)

    Demirbas, Ayhan

    2011-01-01

    The cost of biodiesels varies depending on the feedstock, geographic area, methanol prices, and seasonal variability in crop production. Most of the biodiesel is currently made from soybean, rapeseed, and palm oils. However, there are large amounts of low-cost oils and fats (e.g., restaurant waste, beef tallow, pork lard, and yellow grease) that could be converted to biodiesel. The crop types, agricultural practices, land and labor costs, plant sizes, processing technologies and government policies in different regions considerably vary ethanol production costs and prices by region. The cost of producing bioethanol in a dry mill plant currently totals US$1.65/galon. The largest ethanol cost component is the plant feedstock. It has been showed that plant size has a major effect on cost. The plant size can reduce operating costs by 15-20%, saving another $0.02-$0.03 per liter. Thus, a large plant with production costs of $0.29 per liter may be saving $0.05-$0.06 per liter over a smaller plant. Viscosity of biofuel and biocrude varies greatly with the liquefaction conditions. The high and increasing viscosity indicates a poor flow characteristic and stability. The increase in the viscosity can be attributed to the continuing polymerization and oxidative coupling reactions in the biocrude upon storage. Although stability of biocrude is typically better than that of bio-oil, the viscosity of biocrude is much higher. The bio-oil produced by flash pyrolysis is a highly oxygenated mixture of carbonyls, carboxyls, phenolics and water. It is acidic and potentially corrosive. Bio-oil can also be potentially upgraded by hydrodeoxygenation. The liquid, termed biocrude, contains 60% carbon, 10-20 wt.% oxygen and 30-36 MJ/kg heating value as opposed to <1 wt.% and 42-46 MJ/kg for petroleum. (author)

  20. Competitive liquid biofuels from biomass

    Energy Technology Data Exchange (ETDEWEB)

    Demirbas, Ayhan [Sirnak University, Dean of Engineering Faculty, Department of Mechanical Engineering, Sirnak (Turkey)

    2011-01-15

    The cost of biodiesels varies depending on the feedstock, geographic area, methanol prices, and seasonal variability in crop production. Most of the biodiesel is currently made from soybean, rapeseed, and palm oils. However, there are large amounts of low-cost oils and fats (e.g., restaurant waste, beef tallow, pork lard, and yellow grease) that could be converted to biodiesel. The crop types, agricultural practices, land and labor costs, plant sizes, processing technologies and government policies in different regions considerably vary ethanol production costs and prices by region. The cost of producing bioethanol in a dry mill plant currently totals US$1.65/galon. The largest ethanol cost component is the plant feedstock. It has been showed that plant size has a major effect on cost. The plant size can reduce operating costs by 15-20%, saving another $0.02-$0.03 per liter. Thus, a large plant with production costs of $0.29 per liter may be saving $0.05-$0.06 per liter over a smaller plant. Viscosity of biofuel and biocrude varies greatly with the liquefaction conditions. The high and increasing viscosity indicates a poor flow characteristic and stability. The increase in the viscosity can be attributed to the continuing polymerization and oxidative coupling reactions in the biocrude upon storage. Although stability of biocrude is typically better than that of bio-oil, the viscosity of biocrude is much higher. The bio-oil produced by flash pyrolysis is a highly oxygenated mixture of carbonyls, carboxyls, phenolics and water. It is acidic and potentially corrosive. Bio-oil can also be potentially upgraded by hydrodeoxygenation. The liquid, termed biocrude, contains 60% carbon, 10-20 wt.% oxygen and 30-36 MJ/kg heating value as opposed to <1 wt.% and 42-46 MJ/kg for petroleum. (author)

  1. Import of biofuels and peat

    International Nuclear Information System (INIS)

    Albertsson, N.

    1993-06-01

    In areas neighbouring Sweden, i.e., foremost the Baltic States, it is probable that a large part of the available amounts will be consumed on the domestic market. Studies of the possible use of wood fuel in Estonia, Latvia and Lithuania are being made by the World Bank. Considerable investments will probably be made in the near future to replace existing coal- and oil-fired boiler plants with plants burning wood fuel. Consequently, the opportunities for exports of wood fuel will probably be small. In a global perspective, peat is used only to a limited extent as fuel. In the former Soviet Union alone it is estimated that the amount of peat that is economically feasible to extract is about 166x10 9 tonnes at a moisture content of 40%. Among the most interesting bio products that can be used in energy production from different food processing industries are nut-shells and fruit stones. Some stones, such as those in olives, plums and peaches, are excellent as fuels. The advantage with olive stones, in comparison with chips is that the bulk weight is high and the moisture content is low. Olive stones are thus similar to processed biofuels such as pellets. Due to their high energy content the olive stones can replace coal, which cannot be done by unprocessed fuels without expensive investments in materials handling equipment. Our survey shows that processed forest fuels and crushed olive stones are the products of greatest interest for the Swedish market. It also shows that both chips and peat-based products from the Baltic States are competitive

  2. Competitivity of biofuels in heating

    International Nuclear Information System (INIS)

    Flyktman, M.

    1996-01-01

    The competitivity of indigenous fuels in heating of residential houses in comparison with imported fuels, and both electricity and district heating, has been studied in this research, ordered by the Finnish Ministry of Trade and Industry. Heating plants of residential house scale (20-1000 kW) have been investigated in the research. Only the new heating plants are included in the investigation. The heat generation calculations concerning the residential heating plants have been made for following indigenous fuels: sod peat, fuel-chips, peat and wood pellets, firewood and straw. In addition to these, the calculations have been made for light fuel-oil, electric heating, district heating and natural gas. The local energy tariffs have to be taken into account in electric heating, district heating and natural gas heating. A calculation model, based on flowsheet calculation, forms the main result of the project. By using the model it is possible to update the competitivity data rapidly. Of all the indigenous fuels, sod peat and fuel-chips appeared to be competitive with electric and district heating costs in nearly all scales investigated. The construction of the heat generation costs of solid indigenous fuels differs remarkably from those of electric and district heating. The main part of the heating costs of wood chips and sod peat is formed of fixed costs; i.e. of investment costs and of the costs of heating and control work. The energy costs are the highest costs items in electric an district heating, as well as in the oil heating. It is possible to improve the competitivity of biofuels by developing cheaper boilers and fuel processing and storage devices

  3. Microfluidic biolector-microfluidic bioprocess control in microtiter plates.

    Science.gov (United States)

    Funke, Matthias; Buchenauer, Andreas; Schnakenberg, Uwe; Mokwa, Wilfried; Diederichs, Sylvia; Mertens, Alan; Müller, Carsten; Kensy, Frank; Büchs, Jochen

    2010-10-15

    In industrial-scale biotechnological processes, the active control of the pH-value combined with the controlled feeding of substrate solutions (fed-batch) is the standard strategy to cultivate both prokaryotic and eukaryotic cells. On the contrary, for small-scale cultivations, much simpler batch experiments with no process control are performed. This lack of process control often hinders researchers to scale-up and scale-down fermentation experiments, because the microbial metabolism and thereby the growth and production kinetics drastically changes depending on the cultivation strategy applied. While small-scale batches are typically performed highly parallel and in high throughput, large-scale cultivations demand sophisticated equipment for process control which is in most cases costly and difficult to handle. Currently, there is no technical system on the market that realizes simple process control in high throughput. The novel concept of a microfermentation system described in this work combines a fiber-optic online-monitoring device for microtiter plates (MTPs)--the BioLector technology--together with microfluidic control of cultivation processes in volumes below 1 mL. In the microfluidic chip, a micropump is integrated to realize distinct substrate flow rates during fed-batch cultivation in microscale. Hence, a cultivation system with several distinct advantages could be established: (1) high information output on a microscale; (2) many experiments can be performed in parallel and be automated using MTPs; (3) this system is user-friendly and can easily be transferred to a disposable single-use system. This article elucidates this new concept and illustrates applications in fermentations of Escherichia coli under pH-controlled and fed-batch conditions in shaken MTPs. Copyright 2010 Wiley Periodicals, Inc.

  4. Biofuel and Food-Commodity Prices

    Directory of Open Access Journals (Sweden)

    David Zilberman

    2012-09-01

    Full Text Available The paper summarizes key findings of alternative lines of research on the relationship between food and fuel markets, and identifies gaps between two bodies of literature: one that investigates the relationship between food and fuel prices, and another that investigates the impact of the introduction of biofuels on commodity-food prices. The former body of literature suggests that biofuel prices do not affect food-commodity prices, but the latter suggests it does. We try to explain this gap, and then show that although biofuel was an important contributor to the recent food-price inflation of 2001–2008, its effect on food-commodity prices declined after the recession of 2008/09. We also show that the introduction of cross-price elasticity is important when explaining soybean price, but less so when explaining corn prices.

  5. Next generation biofuel engineering in prokaryotes

    Science.gov (United States)

    Gronenberg, Luisa S.; Marcheschi, Ryan J.; Liao, James C.

    2014-01-01

    Next-generation biofuels must be compatible with current transportation infrastructure and be derived from environmentally sustainable resources that do not compete with food crops. Many bacterial species have unique properties advantageous to the production of such next-generation fuels. However, no single species possesses all characteristics necessary to make high quantities of fuels from plant waste or CO2. Species containing a subset of the desired characteristics are used as starting points for engineering organisms with all desired attributes. Metabolic engineering of model organisms has yielded high titer production of advanced fuels, including alcohols, isoprenoids and fatty acid derivatives. Technical developments now allow engineering of native fuel producers, as well as lignocellulolytic and autotrophic bacteria, for the production of biofuels. Continued research on multiple fronts is required to engineer organisms for truly sustainable and economical biofuel production. PMID:23623045

  6. Bio-fuels of the first generation

    International Nuclear Information System (INIS)

    2012-04-01

    After having briefly recalled the objective of use of renewable energies and the role bio-fuels may play, this publication briefly presents various bio-fuels: bio-diesel (from colza, soybean or sunflower oil), and ethanol (from beet, sugar cane, wheat or corn). Some key data regarding bio-fuel production and use in France are briefly commented. The publication outlines strengths (a positive energy assessment, a decreased dependency on imported fossil fuels and a higher supply safety, a diversification of agriculture revenues and prospects, a reduction of greenhouse gas emissions) and weaknesses (uncertainty regarding the evolution of soil use, an environmental impact related to farming methods) of this sector. Actions undertaken by the ADEME in collaboration with other agencies and institutions are briefly overviewed

  7. MAIN TRENDS OF BIOFUELS PRODUCTION IN UKRAINE

    Directory of Open Access Journals (Sweden)

    Myroslav PANCHUK

    2017-12-01

    Full Text Available The analysis of biological resources for biofuels production in Ukraine has been carried out, and it has been shown that usage of alternative energy sources has great potential for substantially improving energy supply of the state and solving environmental problems. The directions of development and new technologies of obtaining motor fuels from biomass are systematized. It has been established that usage of different types of biofuels and their mixtures for feeding internal combustion engines involves application of modified engines in terms of structure and algorithms and usage of traditional designs of cars without significant structural changes. Moreover, the impact of biofuels on the efficient operation of the engine requires further integrated research.

  8. Biofuels barometer - EurObserv'ER - July 2011

    International Nuclear Information System (INIS)

    2011-07-01

    13,6 % the increase in EU biofuel consumption in 2010. In 2010 biofuel continued to gnaw away at petrol and diesel consumption in the European Union. However its pace backs the assertion that EU biofuel consumption growth slackened off. In the transport sector, it increased by only 1.7 Mtoe compared to 2.7 Mtoe in 2009. The final total biofuel consumption figure for 2010 should hover at around 13,9 Mtoe

  9. Institutional analysis of biofuel production in Northern Ghana

    OpenAIRE

    Kwoyiga, Lydia

    2013-01-01

    The thesis studied the nature of institutional arrangement around biofuel production and how this arrangement has shaped the production outcome of biofuel companies and community development. The study was conducted in two communities of the Yendi Municipal Assembly of the Northern Region of Ghana. In this area, a biofuel company called Biofuel Africa Limited has acquired areas of land and cultivated Jatropha plantations. A total of 32 informants were interviewed to arrive at information ne...

  10. The Roundtable on Sustainable Biofuels: plant scientist input needed.

    Science.gov (United States)

    Haye, Sébastien; Hardtke, Christian S

    2009-08-01

    The Energy Center at the Ecole Polytechnique Fédérale de Lausanne (Swiss federal institute of technology) is coordinating a multi-stakeholder effort, the Roundtable on Sustainable Biofuels (http://energycenter.epfl.ch/biofuels), to develop global standards for sustainable biofuels production and processing. Given that many of the aspects related to biofuel production request a high scientific level of understanding, it is crucial that scientists take part in the discussion.

  11. Particle Manipulation Methods in Droplet Microfluidics.

    Science.gov (United States)

    Tenje, Maria; Fornell, Anna; Ohlin, Mathias; Nilsson, Johan

    2018-02-06

    This Feature describes the different particle manipulation techniques available in the droplet microfluidics toolbox to handle particles encapsulated inside droplets and to manipulate whole droplets. We address the advantages and disadvantages of the different techniques to guide new users.

  12. Microfluidic Analytical Separator for Proteomics, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed innovation is a microfluidic device designed to effect a 2-dimensional resolution of a mixture of proteins based on isoelectric point (pI) and molecular...

  13. Microfluidic Multichannel Flow Cytometer, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed innovation is a "Microfluidic Multichannel Flow Cytometer." Several novel concepts are integrated to produce the final design, which is compatible with...

  14. Optical bio-sensors in microfluidic chips

    NARCIS (Netherlands)

    Pollnau, Markus; Dongre, C.; Pham Van So, P.V.S.; Bernhardi, Edward; Worhoff, Kerstin; de Ridder, R.M.; Hoekstra, Hugo

    2012-01-01

    Direct femtosecond laser writing is used to integrate optical waveguides that intersect the microfluidic channels in a commercial optofluidic chip. With laser excitation, fluorescently labeled DNA molecules of different sizes are separated by capillary electrophoresis with high operating speed and

  15. Microfluidic Analytical Separator for Proteomics, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — SHOT proposes an innovative microfluidic device designed to effect a 2-dimensional resolution of a mixture of proteins based on isoelectric point (pI) and molecular...

  16. A Microfluidic Approach for Studying Piezo Channels.

    Science.gov (United States)

    Maneshi, M M; Gottlieb, P A; Hua, S Z

    2017-01-01

    Microfluidics is an interdisciplinary field intersecting many areas in engineering. Utilizing a combination of physics, chemistry, biology, and biotechnology, along with practical applications for designing devices that use low volumes of fluids to achieve high-throughput screening, is a major goal in microfluidics. Microfluidic approaches allow the study of cells growth and differentiation using a variety of conditions including control of fluid flow that generates shear stress. Recently, Piezo1 channels were shown to respond to fluid shear stress and are crucial for vascular development. This channel is ideal for studying fluid shear stress applied to cells using microfluidic devices. We have developed an approach that allows us to analyze the role of Piezo channels on any given cell and serves as a high-throughput screen for drug discovery. We show that this approach can provide detailed information about the inhibitors of Piezo channels. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Biofuel Feedstock Assessment for Selected Countries

    Energy Technology Data Exchange (ETDEWEB)

    Kline, K.L.; Oladosu, G.A.; Wolfe, A.K.; Perlack, R.D.; Dale, V.H.

    2008-02-18

    Findings from biofuel feedstock production assessments and projections of future supply are presented and discussed. The report aims to improve capabilities to assess the degree to which imported biofuel could contribute to meeting future U.S. targets to reduce dependence on imported oil. The study scope was focused to meet time and resource requirements. A screening process identified Argentina, Brazil, Canada, China, Colombia, India, Mexico, and the Caribbean Basin Initiative (CBI) region for initial analysis, given their likely role in future feedstock supply relevant to U.S. markets. Supply curves for selected feedstocks in these countries are projected for 2012, 2017 and 2027. The supply functions, along with calculations to reflect estimated supplies available for export and/or biofuel production, were provided to DOE for use in a broader energy market allocation study. Potential cellulosic supplies from crop and forestry residues and perennials were also estimated for 2017 and 2027. The analysis identified capacity to potentially double or triple feedstock production by 2017 in some cases. A majority of supply growth is derived from increasing the area cultivated (especially sugarcane in Brazil). This is supplemented by improving yields and farming practices. Most future supplies of corn and wheat are projected to be allocated to food and feed. Larger shares of future supplies of sugarcane, soybean and palm oil production will be available for export or biofuel. National policies are catalyzing investments in biofuel industries to meet targets for fuel blending that generally fall in the 5-10% range. Social and environmental concerns associated with rapid expansion of feedstock production are considered. If the 2017 projected feedstock supply calculated as ‘available’ for export or biofuel were converted to fuel, it would represent the equivalent of about 38 billion gallons of gasoline. Sugarcane and bagasse dominate the available supply, representing 64

  18. Prospects of using algae in biofuel production

    Directory of Open Access Journals (Sweden)

    Y. I. Maltsev

    2017-08-01

    Full Text Available The development of industry, agriculture and the transport sector is associated with the use of various energy sources. Renewable energy sources, including biofuels, are highly promising in this respect. As shown by a number of scientific studies, a promising source for biofuel production that would meet modern requirements may be algal biomass. After activation of the third generation biodiesel production it was assumed that the algae would become the most advantageous source, because it is not only able to accumulate significant amounts of lipids, but could reduce the of agricultural land involved in biofuel production and improve air quality by sequestering CO2. However, a major problem is presented by the cost of algae biomass cultivation and its processing compared to the production of biodiesel from agricultural crops. In this regard, there are several directions of increasing the efficiency of biodiesel production from algae biomass. The first direction is to increase lipid content in algae cells by means of genetic engineering. The second direction is connected with the stimulation of increased accumulation of lipids by stressing algae. The third direction involves the search for new, promising strains of algae that will be characterized by faster biomass accumulation rate, higher content of TAG and the optimal proportions of accumulated saturated and unsaturated fatty acids compared to the already known strains. Recently, a new approach in the search for biotechnologically valuable strains of algae has been formed on the basis of predictions of capacity for sufficient accumulation of lipids by clarifying the evolutionary relationships within the major taxonomic groups of algae. The outcome of these studies is the rapid cost reduction of biofuel production based on algae biomass. All this emphasizes the priority of any research aimed at both improving the process of production of biofuels from algae, and the search for new sources for

  19. Assessing the biofuel options for Southern Africa

    CSIR Research Space (South Africa)

    Von Malititz, GP

    2008-11-01

    Full Text Available with nested levels of resource use rights. Despite the fact that this land is under-producing from a commercial agricultural perspective, this does not automatically translate into this land being available for biofuels. Due to the complex nature... the Biofuel yield in l/ha used in table one, using sugar cane and Jatropha as feedstock. These values are therefore not linked to specific country level growth conditions and assume suitable land is available. 3 It is very difficult to estimate total job...

  20. Microspora floccosa; a potential biofuel producer

    International Nuclear Information System (INIS)

    Memon, A.A.S.; Pathan, A.A.

    2016-01-01

    The current study is focused on biofuel production from local specie of algae. Initially samples were observed to identify the algal specie. Afterward oil was extracted from algae by Soxhlet extraction method, retention time was optimized to improve the yield of oil at different intervals. The recovered oil from algae was subjected to qualitative analysis by Gas Chromatography. Four major peaks were appeared on GC chromatogram which correspond to methyl esters of dodecanoic acid, tetradecanoic acid, 8,11,14-Eicosadienoic acid and 9,10-dihydroxy octadecanoic. The results reflect that Microspora floccosa algae considered to be favorable for biofuel production. (author)

  1. Microspora Floccosa; A Potential Biofuel Producer

    Directory of Open Access Journals (Sweden)

    Aisha Abdul Sattar Memon

    2016-06-01

    Full Text Available The current study is focused on biofuel production from local specie of algae. Initially samples were observed to identify the algal specie. Afterward oil was extracted from algae by Soxhlet extraction method, retention time was optimized to improve the yield of oil at different intervals. The recovered oil from algae was subjected to qualitative analysis by Gas Chromatography. Four major peaks were appeared on GC chromatogram which correspond to methyl esters of Dodecanoic acid, Tetradecanoic acid, 8,11,14-Eicosadienoic acid and 9,10-Dihydroxy octadecanoic. The results reflect that Microspora floccosa algae considered to be favorable for biofuel production.

  2. Water use implications of biofuel scenarios

    Science.gov (United States)

    Teter, J.; Mishra, G. S.; Yeh, S.

    2012-12-01

    Existing studies rely upon attributional lifecycle analysis (LCA) approaches to estimate water intensity of biofuels in liters of irrigated/evapotranspiration water consumed for biofuel production. Such approaches can be misleading. From a policy perspective, a better approach is to compare differential water impacts among scenarios on a landscape scale. We address the shortcomings of existing studies by using consequential LCA, and incorporate direct and indirect land use (changes) of biofuel scenarios, marginal vs. average biofuel water use estimates, future climate, and geographic heterogeneity. We use the outputs of a partial equilibrium economic model, climate and soil data, and a process-based crop-soil-climate-water model to estimate differences in green water (GW - directly from precipitation to soil) and blue water (BW - supplied by irrigation) use among three scenarios: (1) business-as-usual (BAU), (2) Renewable Fuels Standard (RFS) mandates, and (3) a national Low Carbon Fuel Standard (LCFS) plus the RFS scenario. We use spatial statistical methods to interpolate key climatic variables using daily climate observations for the contiguous USA. Finally, we use FAO's crop model AquaCrop to estimate the domestic GW and BW impacts of biofuel policies from 2007-2035. We assess the differences among scenarios along the following metrics: (1) crop area expansion at the county level, including prime and marginal lands, (2) crop-specific and overall annual/seasonal water balances including (a) water inflows (irrigation & precipitation), (b) crop-atmosphere interactions: (evaporation & transpiration) and (d) soil-water flows (runoff & soil infiltration), in mm 3 /acre over the relevant time period. The functional unit of analysis is the BW and GW requirements of biofuels (mm3 per Btu biofuel) at the county level. Differential water use impacts among scenarios are a primarily a function of (1) land use conversion, in particular that of formerly uncropped land classes

  3. Gas Emissions in Combustion of Biofuel

    Directory of Open Access Journals (Sweden)

    Vitázek Ivan

    2014-10-01

    Full Text Available Nowadays, biomass or more precisely biofuel is more and more being exploited as a substitute for fossil fuels for heating as well as for example for heating a drying environment. This contribution focuses on assessing a heat source by combusting various types of solid biofuels. It is a boiler VIGAS 25 with AK 2000 regulation for heating a family house. Gaseous emissions were measured using a device TESTO 330-2LL. Firewood, peat briquettes, bark briquettes and hardwood briquettes were burnt. Results of experimental measurements concerning the production of gaseous emissions are processed in tables and graphs depending on boiler performance and combustion time.

  4. Improving Biofuels Recovery Processes for Energy Efficiency and Sustainability

    Science.gov (United States)

    Biofuels are made from living or recently living organisms. For example, ethanol can be made from fermented plant materials. Biofuels have a number of important benefits when compared to fossil fuels. Biofuels are produced from renewable energy sources such as agricultural resou...

  5. 75 FR 11836 - Bioenergy Program for Advanced Biofuels

    Science.gov (United States)

    2010-03-12

    ... Biofuels AGENCY: Rural Business-Cooperative Service (RBS), USDA. ACTION: Notice of Contract for Proposal... Year 2009 for the Bioenergy Program for Advanced Biofuels under criteria established in the prior NOCP... Bioenergy Program for Advanced Biofuels. In response to the previously published NOCP, approximately $14.5...

  6. A viable technology to generate third-generation biofuel

    DEFF Research Database (Denmark)

    Singh, Anoop; Olsen, Stig Irving; Nigam, Poonam Singh

    2011-01-01

    First generation biofuels are commercialized at large as the production technologies are well developed. However, to grow the raw materials, there is a great need to compromise with food security, which made first generation biofuels not so much promising. The second generation of biofuels does...

  7. Biofuels barometer - EurObserv'ER - July 2016

    International Nuclear Information System (INIS)

    2016-07-01

    The European biofuel market is now regulated by the directive, known as ILUC, whose wording focuses on the environmental impact of first generation biofuel development. This long-awaited clarification has arrived against the backdrop of falling oil prices and shrinking European Union biofuel consumption, which should drop by 1.7% between 2014 and 2015, according to EurObserv'ER

  8. Positive and negative impacts of agricultural production of liquid biofuels

    NARCIS (Netherlands)

    Reijnders, L.; Hester, R.E.; Harrison, R.M.

    2012-01-01

    Agricultural production of liquid biofuels can have positive effects. It can decrease dependence on fossil fuels and increase farmers’ incomes. Agricultural production of mixed perennial biofuel crops may increase pollinator and avian richness. Most types of agricultural crop-based liquid biofuel

  9. Materials for Microfluidic Immunoassays: A Review.

    Science.gov (United States)

    Mou, Lei; Jiang, Xingyu

    2017-08-01

    Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user-friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches. This review focuses on the materials-related aspects of the recent advances in microfluidics-based immunoassays for point-of-care (POC) diagnostics of biomarkers. We compare the materials for microfluidic chips fabrication in five aspects: fabrication, integration, function, modification and cost, and describe their advantages and drawbacks. In addition, we review materials for modifying antibodies to improve the performance of the reaction of immunoassay. We also review the state of the art in microfluidic immunoassays POC platforms, from the laboratory to routine clinical practice, and also commercial products in the market. Finally, we discuss the current challenges and future developments in microfluidic immunoassays. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Micro-optics for microfluidic analytical applications.

    Science.gov (United States)

    Yang, Hui; Gijs, Martin A M

    2018-02-19

    This critical review summarizes the developments in the integration of micro-optical elements with microfluidic platforms for facilitating detection and automation of bio-analytical applications. Micro-optical elements, made by a variety of microfabrication techniques, advantageously contribute to the performance of an analytical system, especially when the latter has microfluidic features. Indeed the easy integration of optical control and detection modules with microfluidic technology helps to bridge the gap between the macroscopic world and chip-based analysis, paving the way for automated and high-throughput applications. In our review, we start the discussion with an introduction of microfluidic systems and micro-optical components, as well as aspects of their integration. We continue with a detailed description of different microfluidic and micro-optics technologies and their applications, with an emphasis on the realization of optical waveguides and microlenses. The review continues with specific sections highlighting the advantages of integrated micro-optical components in microfluidic systems for tackling a variety of analytical problems, like cytometry, nucleic acid and protein detection, cell biology, and chemical analysis applications.

  11. Microfluidic Devices in Advanced Caenorhabditis elegans Research

    Directory of Open Access Journals (Sweden)

    Muniesh Muthaiyan Shanmugam

    2016-08-01

    Full Text Available The study of model organisms is very important in view of their potential for application to human therapeutic uses. One such model organism is the nematode worm, Caenorhabditis elegans. As a nematode, C. elegans have ~65% similarity with human disease genes and, therefore, studies on C. elegans can be translated to human, as well as, C. elegans can be used in the study of different types of parasitic worms that infect other living organisms. In the past decade, many efforts have been undertaken to establish interdisciplinary research collaborations between biologists, physicists and engineers in order to develop microfluidic devices to study the biology of C. elegans. Microfluidic devices with the power to manipulate and detect bio-samples, regents or biomolecules in micro-scale environments can well fulfill the requirement to handle worms under proper laboratory conditions, thereby significantly increasing research productivity and knowledge. The recent development of different kinds of microfluidic devices with ultra-high throughput platforms has enabled researchers to carry out worm population studies. Microfluidic devices primarily comprises of chambers, channels and valves, wherein worms can be cultured, immobilized, imaged, etc. Microfluidic devices have been adapted to study various worm behaviors, including that deepen our understanding of neuromuscular connectivity and functions. This review will provide a clear account of the vital involvement of microfluidic devices in worm biology.

  12. Microfluidics for single cell analysis

    DEFF Research Database (Denmark)

    Jensen, Marie Pødenphant

    Isolation and manipulation of single cells have gained an increasing interest from researchers because of the heterogeneity of cells from the same cell culture. Single cell analysis can ensure a better understanding of differences between individual cells and potentially solve a variety of clinical...... problems. In this thesis lab on a chip systems for rare single cell analysis are investigated. The focus was to develop a commercial, disposable device for circulating tumour cell (CTC) analysis. Such a device must be able to separate rare cells from blood samples and subsequently capture the specific...... cells, and simultaneously be fabricated and operated at low costs and be user-friendly. These challenges were addressed through development of two microfluidic devices, one for rare cell isolation based on pinched flow fractionation (PFF) and one for single cell capture based on hydrodynamic trapping...

  13. Capture of DNA in microfluidic channel using magnetic beads: increasing capture efficiency with integrated microfluidic mixer

    DEFF Research Database (Denmark)

    Lund-Olesen, Torsten; Dufva, Hans Martin; Hansen, Mikkel Fougt

    2007-01-01

    We have studied the hybridization of target DNA in solution with probe DNA on magnetic beads immobilized on the channel sidewalls in a magnetic bead separator. The hybridization is carried out under a liquid flow and is diffusion limited. Two systems are compared: one with a straight microfluidic...... place on the surface in a microfluidic system....

  14. Glucose-based Biofuel Cells: Nanotechnology as a Vital Science in Biofuel Cells Performance

    OpenAIRE

    Hamideh Aghahosseini; Ali Ramazani; Pegah Azimzadeh Asiabi; Farideh Gouranlou; Fahimeh Hosseini; Aram Rezaei; Bong-Ki Min; Sang Woo Joo

    2016-01-01

    Nanotechnology has opened up new opportunities for the design of nanoscale electronic devices suitable for developing high-performance biofuel cells. Glucose-based biofuel cells as green energy sources can be a powerful tool in the service of small-scale power source technology as it provides a latent potential to supply power for various implantable medical electronic devices. By using physiologically produced glucose as a fuel, the living battery can recharge for continuous production of el...

  15. Enzymatic deconstruction of xylan for biofuel production

    Science.gov (United States)

    DODD, DYLAN; CANN, ISAAC K. O.

    2010-01-01

    The combustion of fossil-derived fuels has a significant impact on atmospheric carbon dioxide (CO2) levels and correspondingly is an important contributor to anthropogenic global climate change. Plants have evolved photosynthetic mechanisms in which solar energy is used to fix CO2 into carbohydrates. Thus, combustion of biofuels, derived from plant biomass, can be considered a potentially carbon neutral process. One of the major limitations for efficient conversion of plant biomass to biofuels is the recalcitrant nature of the plant cell wall, which is composed mostly of lignocellulosic materials (lignin, cellulose, and hemicellulose). The heteropolymer xylan represents the most abundant hemicellulosic polysaccharide and is composed primarily of xylose, arabinose, and glucuronic acid. Microbes have evolved a plethora of enzymatic strategies for hydrolyzing xylan into its constituent sugars for subsequent fermentation to biofuels. Therefore, microorganisms are considered an important source of biocatalysts in the emerging biofuel industry. To produce an optimized enzymatic cocktail for xylan deconstruction, it will be valuable to gain insight at the molecular level of the chemical linkages and the mechanisms by which these enzymes recognize their substrates and catalyze their reactions. Recent advances in genomics, proteomics, and structural biology have revolutionized our understanding of the microbial xylanolytic enzymes. This review focuses on current understanding of the molecular basis for substrate specificity and catalysis by enzymes involved in xylan deconstruction. PMID:20431716

  16. Use of biofuels in road transport decreases

    International Nuclear Information System (INIS)

    Segers, R.

    2011-01-01

    The use of biofuels decreased from 3.5 percent, for all gasoline and diesel used by road transport in 2009, to 2 percent in 2010. Particularly the use of biodiesel decreased, dropping from 3.5 to 1.5 percent. The use of biogasoline remained stable, catering for 3 percent of all gasoline use. [nl

  17. Exploring new strategies for cellulosic biofuels production

    Science.gov (United States)

    Paul Langan; S. Gnankaran; Kirk D. Rector; Norma Pawley; David T. Fox; Dae Won Cho; Kenneth E. Hammel

    2011-01-01

    A research program has been initiated to formulate new strategies for efficient low-cost lignocellulosic biomass processing technologies for the production of biofuels. This article reviews results from initial research into lignocellulosic biomass structure, recalcitrance, and pretreatment. In addition to contributing towards a comprehensive understanding of...

  18. Impact of biofuels on contrail warming

    Science.gov (United States)

    Caiazzo, Fabio; Agarwal, Akshat; Speth, Raymond L.; Barrett, Steven R. H.

    2017-11-01

    Contrails and contrail-cirrus may be the largest source of radiative forcing (RF) attributable to aviation. Biomass-derived alternative jet fuels are a potentially major way to mitigate the climate impacts of aviation by reducing lifecycle CO2 emissions. Given the up to 90% reduction in soot emissions from paraffinic biofuels, the potential for a significant impact on contrail RF due to the reduction in contrail-forming ice nuclei (IN) remains an open question. We simulate contrail formation and evolution to quantify RF over the United States under different emissions scenarios. Replacing conventional jet fuels with paraffinic biofuels generates two competing effects. First, the higher water emissions index results in an increase in contrail occurrence (~ +8%). On the other hand, these contrails are composed of larger diameter crystals (~ +58%) at lower number concentrations (~ -75%), reducing both contrail optical depth (~ -29%) and albedo (~ -32%). The net changes in contrail RF induced by switching to biofuels range from -4% to +18% among a range of assumed ice crystal habits (shapes). In comparison, cleaner burning engines (with no increase in water emissions index) result in changes to net contrail RF ranging between -13% and +5% depending on habit. Thus, we find that even 67% to 75% reductions in aircraft soot emissions are insufficient to substantially reduce warming from contrails, and that the use of biofuels may either increase or decrease contrail warming—contrary to previous expectations of a significant decrease in warming.

  19. Production of biofuels via hydrothermal conversion

    DEFF Research Database (Denmark)

    Biller, Patrick; Ross, Andrew

    2016-01-01

    as the quality of targeted biofuel is a function of feedstock and operating conditions. The quality of hydrochar influences its uses as a solid fuel while biocrude quality affects its use as a liquid fuel and feedstock for upgrading to drop-in replacement fuels, while HTG produces a syngas rich in either H2...

  20. Coproduction of bioethanol with other biofuels

    DEFF Research Database (Denmark)

    Ahring, Birgitte Kiær; Westermann, Peter

    2007-01-01

    pilot-scale biorefineries for multiple fuel production and also discuss perspectives for further enhancement of biofuel yields from biomass. The major fuels produced in this refinery are ethanol, hydrogen, and methane. We also discuss the applicability of our biorefinery concept as a bolt-on plant...

  1. Electric vehicles need biofuels; Elektroautos brauchen Biotreibstoffe

    Energy Technology Data Exchange (ETDEWEB)

    Engel, Tomi

    2008-09-15

    The debate over electromobility is in full swing. The effects on the electric power grid and on the biofuels industry are quire different than expected, even paradox. (orig.) [German] Die Debatte um Elektromobilitaet ist in vollem Gang. Die Auswirkung auf das Stromnetz und auf die Biotreibstoffbranche sind ganz anders, als man denkt. Sie wirken fast schon paradox. (Orig.)

  2. Future of Liquid Biofuels for APEC Economies

    Energy Technology Data Exchange (ETDEWEB)

    Milbrandt, A.; Overend, R. P.

    2008-05-01

    This project was initiated by APEC Energy Working Group (EWG) to maximize the energy sector's contribution to the region's economic and social well-being through activities in five areas of strategic importance including liquid biofuels production and development.

  3. SOLID BIOFUEL UTILIZATION IN VEGETABLE OIL PRODUCTION

    Directory of Open Access Journals (Sweden)

    Slusarenko V.

    2016-08-01

    Full Text Available The paper deals with questions of creating at JSC “Alimentarmash "in the last 20 years the technological equipment for the production of vegetable oils from oilseeds: from the press for the final spin to mini oilfactory, using as an energy source for heating the liquid coolant (Thermal oil "Arian" of solid biofuels - husk of sunflower seeds.

  4. Biorefineries for chemical and biofuel production

    DEFF Research Database (Denmark)

    Fjerbæk Søtoft, Lene

    crops for biofuel production is research in biorefineries using a whole-crop approach with the aim of having an optimal use of all the components of the specific crop. Looking at rape as a model crop, the components can be used for i.e. bioethanol, biodiesel, biogas, biohydrogen, feed, food and plant...

  5. Designing Sustainable Supply Chains for Biofuels

    Science.gov (United States)

    Driven by the Energy and Independence Act of 2007 mandate to increase production of alternative fuels and to ensure that this increase causes minimal environmental impact, a project to design sustainable biofuel supply chains has been developed. This effort uses life cycle asses...

  6. Public policy and biofuels: The way forward?

    International Nuclear Information System (INIS)

    Charles, Michael B.; Ryan, Rachel; Ryan, Neal; Oloruntoba, Richard

    2007-01-01

    The use of biofuels has been given much attention by governments around the world, especially in increasingly energy-hungry OECD nations. Proponents have argued that they offer various advantages over hydrocarbon-based fuels, especially with respect to reducing dependence on OPEC-controlled oil, minimizing greenhouse gas (GHG) emissions, and ensuring financial and lifestyle continuity to farmers and agriculturally dependent communities. This paper adds to the continuing technical debate by addressing the issue from a holistic public policy perspective. In particular, it looks at the proposed benefits of biofuels, yet also addresses the implications of increased demand on the global and regional environment, in addition to the economic welfare of developing nations. Furthermore, it posits that short-term reliance on biofuels vis-a-vis other alternative energy sources may potentially inhibit the development and maturation of longer-term technologies that have greater potential to correct the harmful effects of fossil-fuel dependence. In light of this, the manifold policy instruments currently employed or proposed by governments in developed nations to promote biofuels emerge as questionable

  7. Boundless Biofuels? Between Environmental Sustainability and Vulnerability

    NARCIS (Netherlands)

    Mol, A.P.J.

    2007-01-01

    Biofuels currently appear to be one of the major controversies in the agriculture/environment nexus, not unlike genetically modified organisms. While some countries (such as Brazil) have for quite some time supported successful large-scale programmes to improve the production and consumption of

  8. Novel biofuel formulations for enhanced vehicle performance

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Dennis [Michigan State Univ., East Lansing, MI (United States); Narayan, Ramani [Michigan State Univ., East Lansing, MI (United States); Berglund, Kris [Michigan State Univ., East Lansing, MI (United States); Lira, Carl [Michigan State Univ., East Lansing, MI (United States); Schock, Harold [Michigan State Univ., East Lansing, MI (United States); Jaberi, Farhad [Michigan State Univ., East Lansing, MI (United States); Lee, Tonghun [Michigan State Univ., East Lansing, MI (United States); Anderson, James [Michigan State Univ., East Lansing, MI (United States); Wallington, Timothy [Michigan State Univ., East Lansing, MI (United States); Kurtz, Eric [Michigan State Univ., East Lansing, MI (United States); Ruona, Will; Hass, Heinz

    2013-08-30

    This interdisciplinary research program at Michigan State University, in collaboration with Ford Motor Company, has explored the application of tailored or designed biofuels for enhanced vehicle performance and reduced emissions. The project has included a broad range of experimental research, from chemical and biological formation of advanced biofuel components to multicylinder engine testing of blended biofuels to determine engine performance parameters. In addition, the project included computation modeling of biofuel physical and combustion properties, and simulation of advanced combustion modes in model engines and in single cylinder engines. Formation of advanced biofuel components included the fermentation of five-carbon and six-carbon sugars to n-butanol and to butyric acid, two four-carbon building blocks. Chemical transformations include the esterification of the butyric acid produced to make butyrate esters, and the esterification of succinic acid with n-butanol to make dibutyl succinate (DBS) as attractive biofuel components. The conversion of standard biodiesel, made from canola or soy oil, from the methyl ester to the butyl ester (which has better fuel properties), and the ozonolysis of biodiesel and the raw oil to produce nonanoate fuel components were also examined in detail. Physical and combustion properties of these advanced biofuel components were determined during the project. Physical properties such as vapor pressure, heat of evaporation, density, and surface tension, and low temperature properties of cloud point and cold filter plugging point were examined for pure components and for blends of components with biodiesel and standard petroleum diesel. Combustion properties, particularly emission delay that is the key parameter in compression ignition engines, was measured in the MSU Rapid Compression Machine (RCM), an apparatus that was designed and constructed during the project simulating the compression stroke of an internal combustion

  9. Driving biofuels from field to fuel tank.

    Science.gov (United States)

    Gura, Trisha

    2009-07-10

    Rising oil prices, fears of global warming, and instability in oil-producing countries have ignited the rush to produce biofuels from plants. The science is progressing rapidly, driven by favorable policies and generous financing, but many hurdles remain before cars and trucks run on "gasohol" or "grassoline."

  10. 76 FR 7935 - Advanced Biofuel Payment Program

    Science.gov (United States)

    2011-02-11

    ... payments. Application materials may be obtained by contacting one of Rural Development's Energy...) number, which can be obtained at no cost via a toll-free request line at 1-866-705-5711 or online at http... producer'' provisions for determining whether an advanced biofuel producer of biogas or solid advanced...

  11. Characterizing Emissions from the Combustion of Biofuels

    Science.gov (United States)

    Emissions from two biofuels, a soy-based biodiesel and an animal-based biodiesel, were measured and compared to emissions from a distillate petroleum fuel oil. The three fuels were burned in a small fire tube boiler designed for use in institutional, commercial, and light industr...

  12. Panorama 2007: Biofuels and their Environmental Performance

    International Nuclear Information System (INIS)

    Prieur-Vernat, A.; His, St.; Bouvart, F.

    2007-01-01

    Today, the development of bio-fuel pathways is closely associated with targets for the reduction of greenhouse gas (GHG) emissions in the transport sector. Well-to-wheel assessments indicate that the use of these automotive fuels of vegetable origin yield definite benefits in terms of GHG emissions and fossil energy consumption compared to petroleum-based automotive fuels. (author)

  13. Biowastes-to-biofuels routes via gasification

    NARCIS (Netherlands)

    Ptasinski, K.J.; Sues Caula, A.; Jurascik, M.; Badeau, J.P.; Levi, A.

    2009-01-01

    Nowadays, biomass has a well-known potential for producing energy calTiers, such as electricity, heat (steam) and transport biofuels. However, biomass availability is rather limited and stochastically distributed. This could be a major problem in demographically dense regions where land is scarce

  14. Grass Pollen Pollution from Biofuels Farming

    Czech Academy of Sciences Publication Activity Database

    Ratajová, A.; Tříska, Jan; Vrchotová, Naděžda; Kolář, L.; Kužel, S.

    2013-01-01

    Roč. 26, č. 4 (2013), s. 199-203 ISSN 2151-321X R&D Projects: GA MŠk(CZ) ED1.1.00/02.0073 Institutional support: RVO:67179843 Keywords : grass pollen pollution * biofuels farming * temperate climate * PK-fertilization * N-fertilization * phenolic Subject RIV: EH - Ecology, Behaviour Impact factor: 0.556, year: 2013

  15. Lessons from first generation biofuels and implications for the sustainability appraisal of second generation biofuels

    International Nuclear Information System (INIS)

    Mohr, Alison; Raman, Sujatha

    2013-01-01

    Aims: The emergence of second generation (2G) biofuels is widely seen as a sustainable response to the increasing controversy surrounding the first generation (1G). Yet, sustainability credentials of 2G biofuels are also being questioned. Drawing on work in Science and Technology Studies, we argue that controversies help focus attention on key, often value-related questions that need to be posed to address broader societal concerns. This paper examines lessons drawn from the 1G controversy to assess implications for the sustainability appraisal of 2G biofuels. Scope: We present an overview of key 1G sustainability challenges, assess their relevance for 2G, and highlight the challenges for policy in managing the transition. We address limitations of existing sustainability assessments by exploring where challenges might emerge across the whole system of bioenergy and the wider context of the social system in which bioenergy research and policy are done. Conclusions: Key lessons arising from 1G are potentially relevant to the sustainability appraisal of 2G biofuels depending on the particular circumstances or conditions under which 2G is introduced. We conclude that sustainability challenges commonly categorised as either economic, environmental or social are, in reality, more complexly interconnected (so that an artificial separation of these categories is problematic). - Highlights: • Controversy surrounding 1G biofuels is relevant to sustainability appraisal of 2G. • Challenges for policy in managing the transition to 2G biofuels are highlighted. • A key lesson is that sustainability challenges are complexly interconnected

  16. Glucose-based Biofuel Cells: Nanotechnology as a Vital Science in Biofuel Cells Performance

    Directory of Open Access Journals (Sweden)

    Hamideh Aghahosseini

    2016-07-01

    Full Text Available Nanotechnology has opened up new opportunities for the design of nanoscale electronic devices suitable for developing high-performance biofuel cells. Glucose-based biofuel cells as green energy sources can be a powerful tool in the service of small-scale power source technology as it provides a latent potential to supply power for various implantable medical electronic devices. By using physiologically produced glucose as a fuel, the living battery can recharge for continuous production of electricity. This review article presents how nanoscience, engineering and medicine are combined to assist in the development of renewable glucose-based biofuel cell systems. Here, we review recent advances and applications in both abiotic and enzymatic glucose biofuel cells with emphasis on their “implantable” and “implanted” types. Also the challenges facing the design and application of glucose-based biofuel cells to convert them to promising replacement candidates for non-rechargeable lithium-ion batteries are discussed. Nanotechnology could make glucose-based biofuel cells cheaper, lighter and more efficient and hence it can be a part of the solutions to these challenges.

  17. A modelling approach to estimate the European biofuel production: from crops to biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Clodic, Melissa [Institute National de la Recherche Agronomique (IFP/INRA), Paris (France). Instituto Frances do Petroleo

    2008-07-01

    Today, in the context of energy competition and climate change, biofuels are promoted as a renewable resource to diversify the energy supply. However, biofuel development remains controversial. Here, we will present a way to make an environmental and economic cost and benefit analysis of European biofuels, from the crops until the marketed products, by using a linear programming optimization modelling approach. To make this European biofuel production model, named AGRAF, possible, we decided to use different independent linear programming optimization models which represent the separate parts of the process: European agricultural production, production of transforming industries and refinery production. To model the agricultural and the refining sections, we have chosen to improve existing and experimented models by adding a biofuel production part. For the transforming industry, we will create a new partial equilibrium model which will represent stake holders such as Sofiproteol, Stereos, etc. Data will then be exchanged between the models to coordinate all the biofuel production steps. Here, we will also focus on spatialization in order to meet certain of our requirements, such as the exchange flux analysis or the determination of transport costs, usually important in an industrial optimization model. (author)

  18. Inhibitory effect of common microfluidic materials on PCR outcome

    KAUST Repository

    Kodzius, Rimantas; Xiao, Kang; Wu, Jinbo; Yi, Xin; Gong, Xiuqing; Foulds, Ian G.; Wen, Weijia

    2012-01-01

    Microfluidic chips have a variety of applications in the biological sciences and medicine. In contrast with traditional experimental approaches, microfluidics entails lower sample and reagent consumption, allows faster reactions and enables

  19. Plastic-Based Structurally Programmable Microfluidic Biochips for Clinical Diagnostics

    National Research Council Canada - National Science Library

    Ahn, Chong H; Nevin, Joseph H; Beaucage, Gregory

    2005-01-01

    ... and reliable measurements of metabolic parameters from a human body with minimum invasion. The fully integrated disposable biochip is capable of precise volume control with smart microfluidic manipulation without costly on-chip microfluidic components...

  20. Rapid microfluidic thermal cycler for nucleic acid amplification

    Science.gov (United States)

    Beer, Neil Reginald; Vafai, Kambiz

    2015-10-27

    A system for thermal cycling a material to be thermal cycled including a microfluidic heat exchanger; a porous medium in the microfluidic heat exchanger; a microfluidic thermal cycling chamber containing the material to be thermal cycled, the microfluidic thermal cycling chamber operatively connected to the microfluidic heat exchanger; a working fluid at first temperature; a first system for transmitting the working fluid at first temperature to the microfluidic heat exchanger; a working fluid at a second temperature, a second system for transmitting the working fluid at second temperature to the microfluidic heat exchanger; a pump for flowing the working fluid at the first temperature from the first system to the microfluidic heat exchanger and through the porous medium; and flowing the working fluid at the second temperature from the second system to the heat exchanger and through the porous medium.

  1. A microfluidic dialysis device for complex biological mixture SERS analysis

    KAUST Repository

    Perozziello, Gerardo; Candeloro, Patrizio; Gentile, Francesco T.; Coluccio, Maria Laura; Tallerico, Marco; De Grazia, Antonio; Nicastri, Annalisa; Perri, Angela Mena; Parrotta, Elvira; Pardeo, Francesca; Catalano, Rossella; Cuda, Giovanni; Di Fabrizio, Enzo M.

    2015-01-01

    In this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological

  2. [Model-based biofuels system analysis: a review].

    Science.gov (United States)

    Chang, Shiyan; Zhang, Xiliang; Zhao, Lili; Ou, Xunmin

    2011-03-01

    Model-based system analysis is an important tool for evaluating the potential and impacts of biofuels, and for drafting biofuels technology roadmaps and targets. The broad reach of the biofuels supply chain requires that biofuels system analyses span a range of disciplines, including agriculture/forestry, energy, economics, and the environment. Here we reviewed various models developed for or applied to modeling biofuels, and presented a critical analysis of Agriculture/Forestry System Models, Energy System Models, Integrated Assessment Models, Micro-level Cost, Energy and Emission Calculation Models, and Specific Macro-level Biofuel Models. We focused on the models' strengths, weaknesses, and applicability, facilitating the selection of a suitable type of model for specific issues. Such an analysis was a prerequisite for future biofuels system modeling, and represented a valuable resource for researchers and policy makers.

  3. Overview of the Estonian Biofuels Association activities

    International Nuclear Information System (INIS)

    Hueues, Meelis

    2000-01-01

    Due to global warming and environment pollution because of widespread use of fossil fuels there are already tendencies to stabilize and decrease the consumption of these energy resources and take into use more renewable energy resources. Estonian Biofuels Association (EBA) is a non-profit association, which was founded on 8. of May 1998 in Tallinn. The EBA is an independent and voluntary alliance of its members. Fields of activity of the EBA are by biofuels research, developing and evaluation to engage environmental, biofuels and energy saving. EBA members are: energy consultants, scientists, as well as fuel suppliers, DH-companies, technology suppliers, energy service companies etc. The members of EBA are involved in different projects in Estonia, where biomass are produced and used for heating, where wood, waste, peat, rape oil and biogas resources are examined and put into use, and also projects which deal with energy saving and environment friendly equipment production for using biofuels. During our short experience we have noticed that people in Estonia have become more aware of biomass and their use, so the development of environment friendly and sustainable energetics will continue in Estonia. Available biofuels in Estonia could compete with fossil fuels if burnt rationally with high technology equipment. EBA members are convinced that biomass have perspective and that they could play an important role in improving Estonian economic and environmental situation. Modem biomass combustion devices are taken into use more the faster general wealth increases and EBA can raise people's awareness of bio fuel subject through special, courses and media. We want Estonian energy policy to develop towards widespread use of renewable energy resources, which would save energy and environment improve nation's foreign trade balance and create jobs mainly in rural areas

  4. Biofuels, vehicle emissions, and urban air quality.

    Science.gov (United States)

    Wallington, Timothy J; Anderson, James E; Kurtz, Eric M; Tennison, Paul J

    2016-07-18

    Increased biofuel content in automotive fuels impacts vehicle tailpipe emissions via two mechanisms: fuel chemistry and engine calibration. Fuel chemistry effects are generally well recognized, while engine calibration effects are not. It is important that investigations of the impact of biofuels on vehicle emissions consider the impact of engine calibration effects and are conducted using vehicles designed to operate using such fuels. We report the results of emission measurements from a Ford F-350 fueled with either fossil diesel or a biodiesel surrogate (butyl nonanoate) and demonstrate the critical influence of engine calibration on NOx emissions. Using the production calibration the emissions of NOx were higher with the biodiesel fuel. Using an adjusted calibration (maintaining equivalent exhaust oxygen concentration to that of the fossil diesel at the same conditions by adjusting injected fuel quantities) the emissions of NOx were unchanged, or lower, with biodiesel fuel. For ethanol, a review of the literature data addressing the impact of ethanol blend levels (E0-E85) on emissions from gasoline light-duty vehicles in the U.S. is presented. The available data suggest that emissions of NOx, non-methane hydrocarbons, particulate matter (PM), and mobile source air toxics (compounds known, or suspected, to cause serious health impacts) from modern gasoline and diesel vehicles are not adversely affected by increased biofuel content over the range for which the vehicles are designed to operate. Future increases in biofuel content when accomplished in concert with changes in engine design and calibration for new vehicles should not result in problematic increases in emissions impacting urban air quality and may in fact facilitate future required emissions reductions. A systems perspective (fuel and vehicle) is needed to fully understand, and optimize, the benefits of biofuels when blended into gasoline and diesel.

  5. Pathways to Carbon-Negative Liquid Biofuels

    Science.gov (United States)

    Woolf, D.; Lehmann, J.

    2017-12-01

    Many climate change mitigation scenarios assume that atmospheric carbon dioxide removal will be delivered at scale using bioenergy power generation with carbon capture and storage (BECCS). However, other pathways to negative emission technologies (NETs) in the energy sector are possible, but have received relatively little attention. Given that the costs, benefits and life-cycle emissions of technologies vary widely, more comprehensive analyses of the policy options for NETs are critical. This study provides a comparative assessment of the potential pathways to carbon-negative liquid biofuels. It is often assumed that that decarbonisation of the transport sector will include use of liquid biofuels, particularly for applications that are difficult to electrify such as aviation and maritime transport. However, given that biomass and land on which to grow it sustainably are limiting factors in the scaling up of both biofuels and NETs, these two strategies compete for shared factors of production. One way to circumvent this competition is carbon-negative biofuels. Because capture of exhaust CO2 in the transport sector is impractical, this will likely require carbon capture during biofuel production. Potential pathways include, for example, capture of CO2 from fermentation, or sequestration of biochar from biomass pyrolysis in soils, in combination with thermochemical or bio-catalytic conversion of syngas to alcohols or alkanes. Here we show that optimal pathway selection depends on specific resource constraints. As land availability becomes increasingly limiting if bioenergy is scaled up—particularly in consideration that abandoned degraded land is widely considered to be an important resource that does not compete with food fiber or habitat—then systems which enhance land productivity by increasing soil fertility using soil carbon sequestration become increasingly preferable compared to bioenergy systems that deplete or degrade the land resource on which they

  6. Utilization of residues from firing with biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Moberg, P O; Nelson, B; Sjoeberg, A

    1983-10-01

    The aim of the project is to analyse various possibilities of using residues from firing with biofuels, to assess the environmental consequences of such use and to prepare proposal and programme for investigations and tests concerning the use and its environmental consequences. Peat fly ashes have been tested in making concrete with promising results. On the other hand it is doubtful if wood ashes can be used, as the lime content is high. Generally can be said that ashes from biofuels are produced by many small combustion plants and that the quality is unequal. It should be possible to use fly ashes as well as bottom ashes from biofuels as borrow material in road works and construction works. Lime and cement are binders used for stabilization of gravel, sand and clay. Coal fly ashes can partly substitute such binders. Ground stabilization is usual in connection with road construction. It is also probable that fly ashes from biofuels can partly be substituted for cement and lime. Higher prices can be obtained for bio ashes used as binders than used as borrow material. Especially wood ashes are considered to be valuable as forest manure. The value roughly estimated by means of comparison to fertilizer is 400 - 800 SEK/ton. Bottom ashes which are an important part of the total ash production have low contents of heavy metals and are thus perhaps suitable for agricultural use. It seems to be possible to substitute fly ashes from biofuel for part of the precipitation chemicals used for reduction of phosphorus and for sludge conditioning at the local water treatment plants. The estimated Swedish production of wood ashes in 1990 could account for 10 per cent of the present production of potash in Europe. There is a need to cover old tailingponds of rests of sulphide ore with an alkaline and dense material. Locally (Dalecarlia) the good properties of fly ashes for this purpose should be utilized.

  7. New microfluidic platform for life sciences in South Africa

    CSIR Research Space (South Africa)

    Hugo, S

    2012-10-01

    Full Text Available is also offered as numerous devices can be implemented on one disc. A variety of components from sample preparation through to detection can be implemented simply and effectively into an integrated microfluidic solution for life sciences. The lab... in the field of centrifugal microfluidics. New microfluidic platform for life sciences in South Africa S. HUGO, K. LAND CSIR Materials Science and Manufacturing P O Box 395, Pretoria 0001, SOUTH AFRICA Email: kland@csir.co.za INTRODUCTION Microfluidic...

  8. Perspectives of microalgal biofuels as a renewable source of energy

    International Nuclear Information System (INIS)

    Kiran, Bala; Kumar, Ritunesh; Deshmukh, Devendra

    2014-01-01

    Highlights: • Microalgae offer solution of wastewater treatment, CO 2 sequestration, and energy crises. • Microalgal biofuel is renewable, nontoxic and environmentally friendly option. • Integration of wastewater treatment with biofuels production has made them more cost effective. • This article details out the potential production process and benefits of microalgal biofuels. - Abstract: Excessive use of fossil fuels to satisfy our rapidly increasing energy demand has created severe environmental problems, such as air pollution, acid rain and global warming. Biofuels are a potential alternative to fossil fuels. First- and second-generation biofuels face criticism due to food security and biodiversity issues. Third-generation biofuels, based on microalgae, seem to be a plausible solution to the current energy crisis, as their oil-producing capability is many times higher than that of various oil crops. Microalgae are the fastest-growing plants and can serve as a sustainable energy source for the production of biodiesel and several other biofuels by conversion of sunlight into chemical energy. Biofuels produced from microalgae are renewable, non-toxic, biodegradable and environment friendly. Microalgae can be grown in open pond systems or closed photobioreactors. Microalgal biofuels are a potential means to keep the development of human activities in synchronization with the environment. The integration of wastewater treatment with biofuel production using microalgae has made microalgal biofuels more attractive and cost effective. A biorefinery approach can also be used to improve the economics of biofuel production, in which all components of microalgal biomass (i.e., proteins, lipids and carbohydrates) are used to produce useful products. The integration of various processes for maximum economic and environmental benefits minimizes the amount of waste produced and the pollution level. This paper presents an overview of various aspects associated with

  9. Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

    DEFF Research Database (Denmark)

    De Vitis, Stefania; Matarise, Giuseppina; Pardeo, Francesca

    2014-01-01

    The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be...

  10. Controlling two-phase flow in microfluidic systems using electrowetting

    NARCIS (Netherlands)

    Gu, H.

    2011-01-01

    Electrowetting (EW)-based digital microfluidic systems (DMF) and droplet-based two-phase flow microfluidic systems (TPF) with closed channels are the most widely used microfluidic platforms. In general, these two approaches have been considered independently. However, integrating the two

  11. Polymer-based platform for microfluidic systems

    Science.gov (United States)

    Benett, William [Livermore, CA; Krulevitch, Peter [Pleasanton, CA; Maghribi, Mariam [Livermore, CA; Hamilton, Julie [Tracy, CA; Rose, Klint [Boston, MA; Wang, Amy W [Oakland, CA

    2009-10-13

    A method of forming a polymer-based microfluidic system platform using network building blocks selected from a set of interconnectable network building blocks, such as wire, pins, blocks, and interconnects. The selected building blocks are interconnectably assembled and fixedly positioned in precise positions in a mold cavity of a mold frame to construct a three-dimensional model construction of a microfluidic flow path network preferably having meso-scale dimensions. A hardenable liquid, such as poly (dimethylsiloxane) is then introduced into the mold cavity and hardened to form a platform structure as well as to mold the microfluidic flow path network having channels, reservoirs and ports. Pre-fabricated elbows, T's and other joints are used to interconnect various building block elements together. After hardening the liquid the building blocks are removed from the platform structure to make available the channels, cavities and ports within the platform structure. Microdevices may be embedded within the cast polymer-based platform, or bonded to the platform structure subsequent to molding, to create an integrated microfluidic system. In this manner, the new microfluidic platform is versatile and capable of quickly generating prototype systems, and could easily be adapted to a manufacturing setting.

  12. Valve Concepts for Microfluidic Cell Handling

    Directory of Open Access Journals (Sweden)

    M. Grabowski

    2010-01-01

    Full Text Available In this paper we present various pneumatically actuated microfluidic valves to enable user-defined fluid management within a microfluidic chip. To identify a feasible valve design, certain valve concepts are simulated in ANSYS to investigate the pressure dependent opening and closing characteristics of each design. The results are verified in a series of tests. Both the microfluidic layer and the pneumatic layer are realized by means of soft-lithographic techniques. In this way, a network of channels is fabricated in photoresist as a molding master. By casting these masters with PDMS (polydimethylsiloxane we get polymeric replicas containing the channel network. After a plasma-enhanced bonding process, the two layers are irreversibly bonded to each other. The bonding is tight for pressures up to 2 bar. The valves are integrated into a microfluidic cell handling system that is designed to manipulate cells in the presence of a liquid reagent (e.g. PEG – polyethylene glycol, for cell fusion. For this purpose a user-defined fluid management system is developed. The first test series with human cell lines show that the microfluidic chip is suitable for accumulating cells within a reaction chamber, where they can be flushed by a liquid medium.

  13. Material Biocompatibility for PCR Microfluidic Chips

    KAUST Repository

    Kodzius, Rimantas; Chang, Donald Choy; Gong, Xiuqing; Wen, Weijia; Wu, Jinbo; Xiao, Kang; Yi, Xin

    2010-01-01

    As part of the current miniaturization trend, biological reactions and processes are being adapted to microfluidics devices. PCR is the primary method employed in DNA amplification, its miniaturization is central to efforts to develop portable devices for diagnostics and testing purposes. A problem is the PCR-inhibitory effect due to interaction between PCR reagents and the surrounding environment, which effect is increased in high-surface-are-to-volume ration microfluidics. In this study, we evaluated the biocompatibility of various common materials employed in the fabrication of microfluidic chips, including silicon, several kinds of silicon oxide, glasses, plastics, wax, and adhesives. Two-temperature PCR was performed with these materials to determine their PCR-inhibitory effect. In most of the cases, addition of bovine serum albumin effectively improved the reaction yield. We also studied the individual PCR components from the standpoint of adsorption. Most of the materials did not inhibit the DNA, whereas they did show noticeable interaction with the DNA polymerase. Our test, instead of using microfluidic devices, can be easily conducted in common PCR tubes using a standard bench thermocycler. Our data supports an overview of the means by which the materials most bio-friendly to microfluidics can be selected.

  14. Material Biocompatibility for PCR Microfluidic Chips

    KAUST Repository

    Kodzius, Rimantas

    2010-04-23

    As part of the current miniaturization trend, biological reactions and processes are being adapted to microfluidics devices. PCR is the primary method employed in DNA amplification, its miniaturization is central to efforts to develop portable devices for diagnostics and testing purposes. A problem is the PCR-inhibitory effect due to interaction between PCR reagents and the surrounding environment, which effect is increased in high-surface-are-to-volume ration microfluidics. In this study, we evaluated the biocompatibility of various common materials employed in the fabrication of microfluidic chips, including silicon, several kinds of silicon oxide, glasses, plastics, wax, and adhesives. Two-temperature PCR was performed with these materials to determine their PCR-inhibitory effect. In most of the cases, addition of bovine serum albumin effectively improved the reaction yield. We also studied the individual PCR components from the standpoint of adsorption. Most of the materials did not inhibit the DNA, whereas they did show noticeable interaction with the DNA polymerase. Our test, instead of using microfluidic devices, can be easily conducted in common PCR tubes using a standard bench thermocycler. Our data supports an overview of the means by which the materials most bio-friendly to microfluidics can be selected.

  15. Microfluidic CODES: a scalable multiplexed electronic sensor for orthogonal detection of particles in microfluidic channels.

    Science.gov (United States)

    Liu, Ruxiu; Wang, Ningquan; Kamili, Farhan; Sarioglu, A Fatih

    2016-04-21

    Numerous biophysical and biochemical assays rely on spatial manipulation of particles/cells as they are processed on lab-on-a-chip devices. Analysis of spatially distributed particles on these devices typically requires microscopy negating the cost and size advantages of microfluidic assays. In this paper, we introduce a scalable electronic sensor technology, called microfluidic CODES, that utilizes resistive pulse sensing to orthogonally detect particles in multiple microfluidic channels from a single electrical output. Combining the techniques from telecommunications and microfluidics, we route three coplanar electrodes on a glass substrate to create multiple Coulter counters producing distinct orthogonal digital codes when they detect particles. We specifically design a digital code set using the mathematical principles of Code Division Multiple Access (CDMA) telecommunication networks and can decode signals from different microfluidic channels with >90% accuracy through computation even if these signals overlap. As a proof of principle, we use this technology to detect human ovarian cancer cells in four different microfluidic channels fabricated using soft lithography. Microfluidic CODES offers a simple, all-electronic interface that is well suited to create integrated, low-cost lab-on-a-chip devices for cell- or particle-based assays in resource-limited settings.

  16. Rapid wasted-free microfluidic fabrication based on ink-jet approach for microfluidic sensing applications

    Science.gov (United States)

    Jarujareet, Ungkarn; Amarit, Rattasart; Sumriddetchkajorn, Sarun

    2016-11-01

    Realizing that current microfluidic chip fabrication techniques are time consuming and labor intensive as well as always have material leftover after chip fabrication, this research work proposes an innovative approach for rapid microfluidic chip production. The key idea relies on a combination of a widely-used inkjet printing method and a heat-based polymer curing technique with an electronic-mechanical control, thus eliminating the need of masking and molds compared to typical microfluidic fabrication processes. In addition, as the appropriate amount of polymer is utilized during printing, there is much less amount of material wasted. Our inkjet-based microfluidic printer can print out the desired microfluidic chip pattern directly onto a heated glass surface, where the printed polymer is suddenly cured. Our proof-of-concept demonstration for widely-used single-flow channel, Y-junction, and T-junction microfluidic chips shows that the whole microfluidic chip fabrication process requires only 3 steps with a fabrication time of 6 minutes.

  17. Biofuel Feedstock Assessment For Selected Countries

    Energy Technology Data Exchange (ETDEWEB)

    Kline, Keith L [ORNL; Oladosu, Gbadebo A [ORNL; Wolfe, Amy K [ORNL; Perlack, Robert D [ORNL; Dale, Virginia H [ORNL

    2008-02-01

    Findings from biofuel feedstock production assessments and projections of future supply are presented and discussed. The report aims to improve capabilities to assess the degree to which imported biofuel could contribute to meeting future U.S. targets to reduce dependence on imported oil. The study scope was focused to meet time and resource requirements. A screening process identified Argentina, Brazil, Canada, China, Colombia, India, Mexico, and the Caribbean Basin Initiative (CBI) region for initial analysis, given their likely role in future feedstock supply relevant to U.S. markets. Supply curves for selected feedstocks in these countries are projected for 2012, 2017 and 2027. The supply functions, along with calculations to reflect estimated supplies available for export and/or biofuel production, were provided to DOE for use in a broader energy market allocation study. Potential cellulosic supplies from crop and forestry residues and perennials were also estimated for 2017 and 2027. The analysis identified capacity to potentially double or triple feedstock production by 2017 in some cases. A majority of supply growth is derived from increasing the area cultivated (especially sugarcane in Brazil). This is supplemented by improving yields and farming practices. Most future supplies of corn and wheat are projected to be allocated to food and feed. Larger shares of future supplies of sugarcane, soybean and palm oil production will be available for export or biofuel. National policies are catalyzing investments in biofuel industries to meet targets for fuel blending that generally fall in the 5-10% range. Social and environmental concerns associated with rapid expansion of feedstock production are considered. If the 2017 projected feedstock supply calculated as 'available' for export or biofuel were converted to fuel, it would represent the equivalent of about 38 billion gallons of gasoline. Sugarcane and bagasse dominate the available supply

  18. Microfluidics of soft granular gels

    Science.gov (United States)

    Nixon, Ryan; Bhattacharjee, Tapomoy; Sawyer, W. Gregory; Angelini, Thomas E.

    Microfluidic methods for encapsulating cells and particles typically involve drop making with two immiscible fluids. The main materials constraint in this approach is surface tension, creating inherent instability between the two fluids. We can eliminate this instability by using miscible inner and outer phases. This is achieved by using granular micro gels which are chemically miscible but physically do not mix. These microgels are yield stress materials, so they flow as solid plugs far from shear gradients, and fluidize where gradients are generated - near an injection nozzle for example. We have found that tuning the yield stress of the material by varying polymer concentration, device performance can be controlled. The solid like behavior of the gel allows us to produces infinitely stable jets that maintain their integrity and configuration over long distances and times. These properties can be combined and manipulated to produce discrete particulate bunches of an inner phase, flowing inside of an outer phase, well enough even to print a Morse code message suspended within flow chambers about a millimeter in diameter moving at millimeters a second.

  19. Controversies, development and trends of biofuel industry in the world

    Directory of Open Access Journals (Sweden)

    WenJun Zhang

    2012-09-01

    Full Text Available Controversies, development and trends of biofuel industry in the world were discussed in present article. First-generation biofuels, i.e., grain and land based biofuels, occupied large areas of arable lands and severely constrained food supplies, are widely disputed. They have been replaced by second-generation biofuels. The raw materials of the second-generation biofuels include plants, straw, grass and other crops and forest residues. However, the cost for production of the second-generation biofuels is higher. Therefore the development of the third-generation biofuels is undergoing. The third-generation technologies use, mainly algae, as raw material to produce bioethanol, biobutanol, biodiesel and hydrogen, and use discarded fruits to produce dimethylfuran, etc. Different countries and regions are experiencing different stages of biofuel industry. In the future the raw materials for biofuel production will be focused on various by-products, wastes, and organisms that have not direct economic benefit for human. Production technologies should be improved or invented to reduce carbon emission and environmental pollution during biofuel production and to reduce production cost.

  20. Design and Testing of Digital Microfluidic Biochips

    CERN Document Server

    Zhao, Yang

    2013-01-01

    This book provides a comprehensive methodology for automated design, test and diagnosis, and use of robust, low-cost, and manufacturable digital microfluidic systems. It focuses on the development of a comprehensive CAD optimization framework for digital microfluidic biochips that unifies different design problems. With the increase in system complexity and integration levels, biochip designers can utilize the design methods described in this book to evaluate different design alternatives, and carry out design-space exploration to obtain the best design point. Describes practical design automation tools that address different design problems (e.g., synthesis, droplet routing, control-pin mapping, testing and diagnosis, and error recovery) in a unified manner; Applies test pattern generation and error-recovery techniques for digital microfluidics-based biochips; Uses real bioassays as evaluation examples, e.g., multiplexed in vitro human physiological fluids diagnostics, PCR, protein crystallization.  

  1. Temperature Sensing in Modular Microfluidic Architectures

    Directory of Open Access Journals (Sweden)

    Krisna C. Bhargava

    2016-01-01

    Full Text Available A discrete microfluidic element with integrated thermal sensor was fabricated and demonstrated as an effective probe for process monitoring and prototyping. Elements were constructed using stereolithography and market-available glass-bodied thermistors within the modular, standardized framework of previous discrete microfluidic elements demonstrated in the literature. Flow rate-dependent response due to sensor self-heating and microchannel heating and cooling was characterized and shown to be linear in typical laboratory conditions. An acid-base neutralization reaction was performed in a continuous flow setting to demonstrate applicability in process management: the ratio of solution flow rates was varied to locate the equivalence point in a titration, closely matching expected results. This element potentially enables complex, three-dimensional microfluidic architectures with real-time temperature feedback and flow rate sensing, without application specificity or restriction to planar channel routing formats.

  2. Downstream bioprocess characterisation within microfluidic devices

    DEFF Research Database (Denmark)

    Marques, Marco; Krühne, Ulrich; Szita, Nicolas

    2016-01-01

    developed which has, to some extent, hindered their implementation as early process development tools. Microfluidic devices are particularly attractive for using fewer resources, for having the possibility of parallelisation and for requiring fewer mechanical manipulations. The expectation...... is that these devices will facilitate the rapid definition of critical process parameters, and thus ultimately reduce production costs. We have developed several microfluidic mDUOs and combined them with advanced and novel analytical approaches, resulting in devices that can potentially be employed for both analytical...... for the liquid–liquid extraction of pharmaceuticals, for the purification and concentration of drug delivery vehicles, and for the flocculation of yeast cells in microfluidic devices. For the latter, we will present for the first time the capability to study flocculation-growth independent from the floc breakage...

  3. Microfluidic Pumps Containing Teflon [Trademark] AF Diaphragms

    Science.gov (United States)

    Willis, Peter; White, Victor; Grunthaner, Frank; Ikeda, Mike; Mathies, Richard A.

    2009-01-01

    Microfluidic pumps and valves based on pneumatically actuated diaphragms made of Teflon AF polymers are being developed for incorporation into laboratory-on-a-chip devices that must perform well over temperature ranges wider than those of prior diaphragm-based microfluidic pumps and valves. Other potential applications include implanted biomedical microfluidic devices, wherein the biocompatability of Teflon AF polymers would be highly advantageous. These pumps and valves have been demonstrated to function stably after cycling through temperatures from -125 to 120 C. These pumps and valves are intended to be successors to similar prior pumps and valves containing diaphragms made of polydimethylsiloxane (PDMS) [commonly known as silicone rubber]. The PDMS-containing valves ae designed to function stably only within the temperature range from 5 to 80 C. Undesirably, PDMS membranes are somwehat porous and retain water. PDMS is especially unsuitable for use at temperatures below 0 C because the formation of ice crystals increases porosity and introduces microshear.

  4. Designing Polymeric Microfluidic Platforms for Biomedical Applications

    DEFF Research Database (Denmark)

    Vedarethinam, Indumathi

    Micro- and Nanotechnology have the potential to offer a smart solution for diagnostics and academia research with rapid, low cost, robust analysis systems to facilitate biological analyses. New, high throughput microfluidic platforms have the potential to surpass in performance the conventional...... analyses systems in use today. The overall goal of this PhD project is to address two different areas using microfluidics : i) Chromosome analysis by metaphase FISH such a platform, if successful, can immediately substitute the routine, labor-intensive, glass slide-based FISH analyses in Clinical...... Cytogenetics laboratories. During the course of this project, initially the suitability of the polymeric chip substrate was tested and a microfluidic device was developed for performing interphase FISH analysis. With this device, the key factors involved in chromosome spreading crucial to FISH analysis were...

  5. Molecular Imaging Probe Development using Microfluidics

    Science.gov (United States)

    Liu, Kan; Wang, Ming-Wei; Lin, Wei-Yu; Phung, Duy Linh; Girgis, Mark D.; Wu, Anna M.; Tomlinson, James S.; Shen, Clifton K.-F.

    2012-01-01

    In this manuscript, we review the latest advancement of microfluidics in molecular imaging probe development. Due to increasing needs for medical imaging, high demand for many types of molecular imaging probes will have to be met by exploiting novel chemistry/radiochemistry and engineering technologies to improve the production and development of suitable probes. The microfluidic-based probe synthesis is currently attracting a great deal of interest because of their potential to deliver many advantages over conventional systems. Numerous chemical reactions have been successfully performed in micro-reactors and the results convincingly demonstrate with great benefits to aid synthetic procedures, such as purer products, higher yields, shorter reaction times compared to the corresponding batch/macroscale reactions, and more benign reaction conditions. Several ‘proof-of-principle’ examples of molecular imaging probe syntheses using microfluidics, along with basics of device architecture and operation, and their potential limitations are discussed here. PMID:22977436

  6. Preparation of nanoparticles by continuous-flow microfluidics

    International Nuclear Information System (INIS)

    Jahn, Andreas; Reiner, Joseph E.; Vreeland, Wyatt N.; DeVoe, Don L.; Locascio, Laurie E.; Gaitan, Michael

    2008-01-01

    We review a variety of micro- and nanoparticle formulations produced with microfluidic methods. A diverse variety of approaches to generate microscale and nanoscale particles has been reported. Here we emphasize the use of microfluidics, specifically microfluidic systems that operate in a continuous flow mode, thereby allowing continuous generation of desired particle formulations. The generation of semiconductor quantum dots, metal colloids, emulsions, and liposomes is considered. To emphasize the potential benefits of the continuous-flow microfluidic methodology for nanoparticle generation, preliminary data on the size distribution of liposomes formed using the microfluidic approach is compared to the traditional bulk alcohol injection method.

  7. Operation placement for application-specific digital microfluidic biochips

    DEFF Research Database (Denmark)

    Alistar, Mirela; Pop, Paul; Madsen, Jan

    2013-01-01

    Microfluidic-based biochips are replacing the conventional biochemical analyzers, and are able to integrate onchip all the necessary functions for biochemical analysis using microfluidics. The digital microfluidic biochips are based on the manipulation of liquids not as a continuous flow......, but as discrete droplets on an array of electrodes. Microfluidic operations, such as transport, mixing, split, are performed on this array by routing the corresponding droplets on a series of electrodes. Researchers have proposed several approaches for the synthesis of digital microfluidic biochips. All previous...

  8. Cyanofuels: biofuels from cyanobacteria. Reality and perspectives.

    Science.gov (United States)

    Sarsekeyeva, Fariza; Zayadan, Bolatkhan K; Usserbaeva, Aizhan; Bedbenov, Vladimir S; Sinetova, Maria A; Los, Dmitry A

    2015-08-01

    Cyanobacteria are represented by a diverse group of microorganisms that, by virtue of being a part of marine and freshwater phytoplankton, significantly contribute to the fixation of atmospheric carbon via photosynthesis. It is assumed that ancient cyanobacteria participated in the formation of earth's oil deposits. Biomass of modern cyanobacteria may be converted into bio-oil by pyrolysis. Modern cyanobacteria grow fast; they do not compete for agricultural lands and resources; they efficiently convert excessive amounts of CO2 into biomass, thus participating in both carbon fixation and organic chemical production. Many cyanobacterial species are easier to genetically manipulate than eukaryotic algae and other photosynthetic organisms. Thus, the cyanobacterial photosynthesis may be directed to produce carbohydrates, fatty acids, or alcohols as renewable sources of biofuels. Here we review the recent achievements in the developments and production of cyanofuels-biofuels produced from cyanobacterial biomass.

  9. Microbial stress tolerance for biofuels. Systems biology

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Zonglin Lewis (ed.) [National Center for Agricultural Utilization Research, USDA-ARS, Peoria, IL (United States)

    2012-07-01

    The development of sustainable and renewable biofuels is attracting growing interest. It is vital to develop robust microbial strains for biocatalysts that are able to function under multiple stress conditions. This Microbiology Monograph provides an overview of methods for studying microbial stress tolerance for biofuels applications using a systems biology approach. Topics covered range from mechanisms to methodology for yeast and bacteria, including the genomics of yeast tolerance and detoxification; genetics and regulation of glycogen and trehalose metabolism; programmed cell death; high gravity fermentations; ethanol tolerance; improving biomass sugar utilization by engineered Saccharomyces; the genomics on tolerance of Zymomonas mobilis; microbial solvent tolerance; control of stress tolerance in bacterial host organisms; metabolomics for ethanologenic yeast; automated proteomics work cell systems for strain improvement; and unification of gene expression data for comparable analyses under stress conditions. (orig.)

  10. Microalgae for Biofuels and Animal Feeds

    Directory of Open Access Journals (Sweden)

    John Benemann

    2013-11-01

    Full Text Available The potential of microalgae biomass production for low-cost commodities—biofuels and animal feeds—using sunlight and CO2 is reviewed. Microalgae are currently cultivated in relatively small-scale systems, mainly for high value human nutritional products. For commodities, production costs must be decreased by an order of magnitude, and high productivity algal strains must be developed that can be stably cultivated in large open ponds and harvested by low-cost processes. For animal feeds, the algal biomass must be high in digestible protein and long-chain omega-3 fatty acids that can substitute for fish meal and fish oils. Biofuels will require a high content of vegetable oils (preferably triglycerides, hydrocarbons or fermentable carbohydrates. Many different cultivation systems, algal species, harvesting methods, and biomass processing technologies are being developed worldwide. However, only raceway-type open pond systems are suitable for the production of low-cost commodities.

  11. Sustainable Process Design of Lignocellulose based Biofuel

    DEFF Research Database (Denmark)

    Mangnimit, Saranya; Malakul, Pomthong; Gani, Rafiqul

    the production and use of alternative and sustainable energy sources as rapidly as possible. Biofuel is a type of alternative energy that can be produced from many sources including sugar substances (such as sugarcane juice and molasses), starchy materials (such as corn and cassava), and lignocellulosic...... materials such as agricultural residual, straw and wood chips, the residual from wood industry. However, those sugar and starchy materials can be used not only to make biofuels but they are also food sources. Thus, lignocellulosic materials are interesting feed-stocls as they are inexpensive, abundantly...... available, and are also non-food crops. In this respect, Cassava rhizome has several characteristics that make it a potential feedstock for fuel ethanol production. It has high content of cellulose and hemicelluloses . The objective of this paper is to present a study focused on the sustainable process...

  12. Applications of Cyanobacteria in Biofuel Production

    DEFF Research Database (Denmark)

    Möllers, K. Benedikt

    and to evolve from a wasteful petrochemical system into a sustainable bio-based society, biofuels and the introduction of bio-refineries play an essential role. Aquatic phototrophs are promising organisms to employ photosynthetic capacities as well as the derived carbohydrates for the production of biofuels......, enzymatic conversion of lignocellulosic biomass for further fermentation or as a platform chemical in a bio-refinery concept. Autotrophically cultivated cells of the marine model cyanobacterium Synechococcus sp. PCC 7002 (Synechococcus) were exposed to mild nitrogen starvation which has been identified...... for fermentation of plant waste material or a substitute for yeast extract. By mimicking photosynthetic electron transport from light excited photo pigments to LPMOs in combination with a reductant and cellulose as substrate, a 100-fold increase in catalytic activity of LPMOs was observed. Also, it was found...

  13. Biofuels, land use change and smallholder livelihoods

    DEFF Research Database (Denmark)

    Hought, Joy Marie; Birch-Thomsen, Torben; Petersen, Jacob

    2012-01-01

    of biofuel feedstock adoption by smallholders in the northwestern Cambodian province of Banteay Meanchey, a region undergoing rapid land use change following the formal end of the Khmer Rouge era in 1989 and subsequent rural resettlement. Remote sensing data combined with field interviews pointed to three...... discrete phases of land use change in this period: first, as a result of the establishment of new settlements (mainly subsistence rice production); second, via the expansion of cash crop cultivation into forested areas (mainly grown on upland fields); and third, due to the response of smallholders...... market had severe consequences for livelihoods and food security. The paper concludes with a discussion of the probable impacts of the emerging cassava market on trajectories in land use, land ownership, and land access in rural Cambodia. The case looks at biofuel adoption in the context of other land...

  14. Drivers for An International Biofuels Market

    International Nuclear Information System (INIS)

    Slingerland, S.; Van Geuns, L.

    2005-12-01

    This paper explores geopolitical and economic drivers for an international biofuels market. It is concluded that the biofuels market so far is primarily regionally oriented and policy driven. However, as demand is expected to increase in the years to come and demand and production do not coincide geographically, an international market is soon expected to arise. How quickly this market will develop is determined by several geopolitical and economic factors. Important geopolitical factors are in particular security of supply and risk abatement considerations, the contents of future emission reduction agreements, and the interaction with in new parties and policies such as those in the agricultural sector. Key economic factors are the prices of primary biomass and petroleum, as well as technological development influencing the price of conversion of biomass to end-use applications. International certification is likely to play a key role in determining whether or not this market will develop in an ecologically sound way.

  15. Bio-fuels: European Communities fiscal initiatives

    International Nuclear Information System (INIS)

    Autrand, A.

    1992-01-01

    This paper first reviews the influence that European Communities fiscal policies have had in the past on the development of more environmentally compatible fuels such as unleaded gasoline. It then discusses which directions fiscal policy makers should take in order to create appropriate financial incentives encouraging the production and use of biomass derived fuels - methanol, ethanol and pure and transesterified vegetable oils. An assessment is made of the efficacy of a recent European Communities proposal which calls for the application of excise tax reductions on bio-fuels. Attention is given to the net effects due to reduced sulfur and carbon dioxide emissions characterizing bio-fuels and the increased use of fertilizers necessary to produce biomass fuels

  16. Climate changes, biofuels and the sustainable future

    International Nuclear Information System (INIS)

    Zidansek, Aleksander; Blinc, Robert; Jeglic, Anton; Kabashi, Skender; Bekteshi, Sadik; Slaus, Ivo

    2009-01-01

    Climate change is one of the most dangerous problems of the contemporary world. We can either adapt to the corresponding changes or try to reduce their impact by significantly reducing fossil fuel burning. A hydrogen-based economy using energy from biomass, solar, wind and other renewable sources and/or nuclear energy seems to be a viable alternative. Here we analyse the possibilities of the biofuels to replace fossil fuels and their potential to contribute to hydrogen economy. (author)

  17. Climate changes, biofuels and the sustainable future

    Energy Technology Data Exchange (ETDEWEB)

    Zidansek, Aleksander; Blinc, Robert [Jozef Stefan International Postgraduate School, Jamova 39, Ljubljana (Slovenia); Jozef Stefan Institute, Jamova 39, Ljubljana (Slovenia); Jeglic, Anton [Faculty of Electrical Engineering, University of Ljubljana (Slovenia); Kabashi, Skender; Bekteshi, Sadik [Faculty of Mathematical and Natural Sciences, University of Prishtina, Kosovo (RS); Slaus, Ivo [Ruder Boskovic Institute, Bijenicka 54, Zagreb (Croatia)

    2009-08-15

    Climate change is one of the most dangerous problems of the contemporary world. We can either adapt to the corresponding changes or try to reduce their impact by significantly reducing fossil fuel burning. A hydrogen-based economy using energy from biomass, solar, wind and other renewable sources and/or nuclear energy seems to be a viable alternative. Here we analyse the possibilities of the biofuels to replace fossil fuels and their potential to contribute to hydrogen economy. (author)

  18. Renewable energy progress and biofuels sustainability

    Energy Technology Data Exchange (ETDEWEB)

    Hamelinck, C.; De Lovinfosse, I.; Koper, M.; Beestermoeller, C.; Nabe, C.; Kimmel, M.; Van den Bos, A.; Yildiz, I.; Harteveld, M. [Ecofys Netherlands, Utrecht (Netherlands); Ragwitz, M.; Steinhilber, S. [Fraunhofer Institut fuer System- und Innovationsforschung ISI, Karlsruhe (Germany); Nysten, J.; Fouquet, D. [Becker Buettner Held BBH, Munich (Germany); Resch, G.; Liebmann, L.; Ortner, A.; Panzer, C. [Energy Economics Group EEG, Vienna University of Technology, Vienna (Austria); Walden, D.; Diaz Chavez, R.; Byers, B.; Petrova, S.; Kunen, E. [Winrock International, Brussels (Belgium); Fischer, G.

    2013-03-15

    On 27 March 2013, the European Commission published its first Renewable Energy Progress Report under the framework of the 2009 Renewable Energy Directive. Since the adoption of this directive and the introduction of legally binding renewable energy targets, most Member States experienced significant growth in renewable energy consumption. 2010 figures indicate that the EU as a whole is on its trajectory towards the 2020 targets with a renewable energy share of 12.7%. Moreover, in 2010 the majority of Member States already reached their 2011/2012 interim targets set in the Directive. However, as the trajectory grows steeper towards the end, more efforts will still be needed from the Member States in order to reach the 2020 targets. With regard to the EU biofuels and bioliquids sustainability criteria, Member States' implementation of the biofuels scheme is considered too slow. In accordance with the reporting requirements set out in the 2009 Directive on Renewable Energy, every two years the European Commission publishes a Renewable Energy Progress Report. The report assesses Member States' progress in the promotion and use of renewable energy along the trajectory towards the 2020 renewable energy targets. The report also describes the overall renewable energy policy developments in each Member State and their compliance with the measures outlined in the Directive and the National Renewable Energy Action Plans. Moreover, in accordance with the Directive, it reports on the sustainability of biofuels and bioliquids consumed in the EU and the impacts of this consumption. A consortium led by Ecofys was contracted by the European Commission to perform support activities concerning the assessment of progress in renewable energy and sustainability of biofuels.

  19. Renewable energy progress and biofuels sustainability

    Energy Technology Data Exchange (ETDEWEB)

    Hamelinck, C.; De Lovinfosse, I.; Koper, M.; Beestermoeller, C.; Nabe, C.; Kimmel, M.; Van den Bos, A.; Yildiz, I.; Harteveld, M. [Ecofys Netherlands, Utrecht (Netherlands); Ragwitz, M.; Steinhilber, S. [Fraunhofer Institut fuer System- und Innovationsforschung ISI, Karlsruhe (Germany); Nysten, J.; Fouquet, D. [Becker Buettner Held BBH, Munich (Germany); Resch, G.; Liebmann, L.; Ortner, A.; Panzer, C. [Energy Economics Group EEG, Vienna University of Technology, Vienna (Austria); Walden, D.; Diaz Chavez, R.; Byers, B.; Petrova, S.; Kunen, E. [Winrock International, Brussels (Belgium); Fischer, G.

    2013-03-15

    On 27 March 2013, the European Commission published its first Renewable Energy Progress Report under the framework of the 2009 Renewable Energy Directive. Since the adoption of this directive and the introduction of legally binding renewable energy targets, most Member States experienced significant growth in renewable energy consumption. 2010 figures indicate that the EU as a whole is on its trajectory towards the 2020 targets with a renewable energy share of 12.7%. Moreover, in 2010 the majority of Member States already reached their 2011/2012 interim targets set in the Directive. However, as the trajectory grows steeper towards the end, more efforts will still be needed from the Member States in order to reach the 2020 targets. With regard to the EU biofuels and bioliquids sustainability criteria, Member States' implementation of the biofuels scheme is considered too slow. In accordance with the reporting requirements set out in the 2009 Directive on Renewable Energy, every two years the European Commission publishes a Renewable Energy Progress Report. The report assesses Member States' progress in the promotion and use of renewable energy along the trajectory towards the 2020 renewable energy targets. The report also describes the overall renewable energy policy developments in each Member State and their compliance with the measures outlined in the Directive and the National Renewable Energy Action Plans. Moreover, in accordance with the Directive, it reports on the sustainability of biofuels and bioliquids consumed in the EU and the impacts of this consumption. A consortium led by Ecofys was contracted by the European Commission to perform support activities concerning the assessment of progress in renewable energy and sustainability of biofuels.

  20. Pretreatment techniques for biofuels and biorefineries

    Energy Technology Data Exchange (ETDEWEB)

    Fang, Zhen (ed.) [Chinese Academy of Sciences, Kunming, YN (China). Xishuangbanna Tropical Botonical Garden

    2013-02-01

    The first book focused on pretreatment techniques for biofuels contributed by the world's leading experts. Extensively covers the different types of biomass, various pretreatment approaches and methods that show the subsequent production of biofuels and chemicals. In addition to traditional pretreatment methods, novel techniques are also introduced and discussed. An accessible reference work for students, researchers, academicians and industrialists in biorefineries. This book includes 19 chapters contributed by the world's leading experts on pretreatment methods for biomass. It extensively covers the different types of biomass (e.g. molasses, sugar beet pulp, cheese whey, sugarcane residues, palm waste, vegetable oil, straws, stalks and wood), various pretreatment approaches (e.g. physical, thermal, chemical, physicochemical and biological) and methods that show the subsequent production of biofuels and chemicals such as sugars, ethanol, extracellular polysaccharides, biodiesel, gas and oil. In addition to traditional methods such as steam, hot-water, hydrothermal, diluted-acid, organosolv, ozonolysis, sulfite, milling, fungal and bacterial, microwave, ultrasonic, plasma, torrefaction, pelletization, gasification (including biogas) and liquefaction pretreatments, it also introduces and discusses novel techniques such as nano and solid catalysts, organic electrolyte solutions and ionic liquids. This book offers a review of state-of-the-art research and provides guidance for the future paths of developing pretreatment techniques of biomass for biofuels, especially in the fields of biotechnology, microbiology, chemistry, materials science and engineering. It intends to provide a systematic introduction of pretreatment techniques. It is an accessible reference work for students, researchers, academicians and industrialists in biorefineries.

  1. National Biofuels Action Plan, October 2008

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2008-10-01

    To help industry achieve the aggressive national goals, Federal agencies will need to continue to enhance their collaboration. The Biomass Research and Development (R&D) Board was created by Congress in the Biomass Research and Development Act of 2000. The National Biofuels Action Plan outlines areas where interagency cooperation will help to evolve bio-based fuel production technologies from promising ideas to competitive solutions.

  2. Panorama 2007: New Bio-fuel Technologies

    International Nuclear Information System (INIS)

    His, St.

    2007-01-01

    New pathways are emerging in the wake of the boom in the biofuels market. Their development is driven by the search for improved product quality and a broader range of natural plant feedstock. Interested in these new pathways, the oil companies are seeking to differentiate themselves on a market that promises to be very competitive in future. This is a turning point in a sector that has historically been dominated by agro-food companies. (author)

  3. Pulsed laser triggered high speed microfluidic switch

    Science.gov (United States)

    Wu, Ting-Hsiang; Gao, Lanyu; Chen, Yue; Wei, Kenneth; Chiou, Pei-Yu

    2008-10-01

    We report a high-speed microfluidic switch capable of achieving a switching time of 10 μs. The switching mechanism is realized by exciting dynamic vapor bubbles with focused laser pulses in a microfluidic polydimethylsiloxane (PDMS) channel. The bubble expansion deforms the elastic PDMS channel wall and squeezes the adjacent sample channel to control its fluid and particle flows as captured by the time-resolved imaging system. A switching of polystyrene microspheres in a Y-shaped channel has also been demonstrated. This ultrafast laser triggered switching mechanism has the potential to advance the sorting speed of state-of-the-art microscale fluorescence activated cell sorting devices.

  4. Integrated Microfluidic Sensor System with Magnetostrictive Resonators

    KAUST Repository

    Liang, Cai; Kosel, Jü rgen; Gooneratne, Chinthaka

    2011-01-01

    The present embodiments describe a method that integrates a magnetostrictive sensor with driving and detecting elements into a microfluidic chip to detect a chemical, biochemical or biomedical species. These embodiments may also measure the properties of a fluid such as viscosity, pH values. The whole system can be referred to lab-on-a-chip (LOC) or micro-total-analysis-systems (.mu.TAS). In particular, this present embodiments include three units, including a microfluidics unit, a magnetostrictive sensor, and driving/detecting elements. An analyzer may also be provided to analyze an electrical signal associated with a feature of a target specimen.

  5. Microfluidic device for acoustic cell lysis

    Science.gov (United States)

    Branch, Darren W.; Cooley, Erika Jane; Smith, Gennifer Tanabe; James, Conrad D.; McClain, Jaime L.

    2015-08-04

    A microfluidic acoustic-based cell lysing device that can be integrated with on-chip nucleic acid extraction. Using a bulk acoustic wave (BAW) transducer array, acoustic waves can be coupled into microfluidic cartridges resulting in the lysis of cells contained therein by localized acoustic pressure. Cellular materials can then be extracted from the lysed cells. For example, nucleic acids can be extracted from the lysate using silica-based sol-gel filled microchannels, nucleic acid binding magnetic beads, or Nafion-coated electrodes. Integration of cell lysis and nucleic acid extraction on-chip enables a small, portable system that allows for rapid analysis in the field.

  6. Integrated Microfluidic Gas Sensors for Water Monitoring

    Science.gov (United States)

    Zhu, L.; Sniadecki, N.; DeVoe, D. L.; Beamesderfer, M.; Semancik, S.; DeVoe, D. L.

    2003-01-01

    A silicon-based microhotplate tin oxide (SnO2) gas sensor integrated into a polymer-based microfluidic system for monitoring of contaminants in water systems is presented. This device is designed to sample a water source, control the sample vapor pressure within a microchannel using integrated resistive heaters, and direct the vapor past the integrated gas sensor for analysis. The sensor platform takes advantage of novel technology allowing direct integration of discrete silicon chips into a larger polymer microfluidic substrate, including seamless fluidic and electrical interconnects between the substrate and silicon chip.

  7. Integrated Microfluidic Sensor System with Magnetostrictive Resonators

    KAUST Repository

    Liang, Cai

    2011-12-08

    The present embodiments describe a method that integrates a magnetostrictive sensor with driving and detecting elements into a microfluidic chip to detect a chemical, biochemical or biomedical species. These embodiments may also measure the properties of a fluid such as viscosity, pH values. The whole system can be referred to lab-on-a-chip (LOC) or micro-total-analysis-systems (.mu.TAS). In particular, this present embodiments include three units, including a microfluidics unit, a magnetostrictive sensor, and driving/detecting elements. An analyzer may also be provided to analyze an electrical signal associated with a feature of a target specimen.

  8. 3D Ceramic Microfluidic Device Manufacturing

    International Nuclear Information System (INIS)

    Natarajan, Govindarajan; Humenik, James N

    2006-01-01

    Today, semiconductor processing serves as the backbone for the bulk of micromachined devices. Precision lithography and etching technology used in the semiconductor industry are also leveraged by alternate techniques like electroforming and molding. The nature of such processing is complex, limited and expensive for any manufacturing foundry. This paper details the technology elements developed to manufacture cost effective and versatile microfluidic devices for applications ranging from medical diagnostics to characterization of bioassays. Two applications using multilayer ceramic technology to manufacture complex 3D microfluidic devices are discussed

  9. Micro-Fluidic Device for Drug Delivery

    Science.gov (United States)

    Beebe, David J. (Inventor); MacDonald, Michael J. (Inventor); Eddington, David T. (Inventor); Mensing, Glennys A. (Inventor)

    2014-01-01

    A microfluidic device is provided for delivering a drug to an individual. The microfluidic device includes a body that defines a reservoir for receiving the drug therein. A valve interconnects the reservoir to an output needle that is insertable into the skin of an individual. A pressure source urges the drug from the reservoir toward the needle. The valve is movable between a closed position preventing the flow of the drug from the reservoir to the output needle and an open position allowing for the flow of the drug from the reservoir to the output needle in response to a predetermined condition in the physiological fluids of the individual.

  10. Microfluidic Apps for off-the-shelf instruments.

    Science.gov (United States)

    Mark, Daniel; von Stetten, Felix; Zengerle, Roland

    2012-07-21

    Within the last decade a huge increase in research activity in microfluidics could be observed. However, despite several commercial success stories, microfluidic chips are still not sold in high numbers in mass markets so far. Here we promote a new concept that could be an alternative approach to commercialization: designing microfluidic chips for existing off-the-shelf instruments. Such "Microfluidic Apps" could significantly lower market entry barriers and provide many advantages: developers of microfluidic chips make use of existing equipment or platforms and do not have to develop instruments from scratch; end-users can profit from microfluidics without the need to invest in new equipment; instrument manufacturers benefit from an expanded customer base due to the new applications that can be implemented in their instruments. Microfluidic Apps could be considered as low-cost disposables which can easily be distributed globally via web-shops. Therefore they could be a door-opener for high-volume mass markets.

  11. Road-map for 2. generation biofuels

    International Nuclear Information System (INIS)

    2009-01-01

    This document presents road-map issues concerning the requirements for biofuels of second generation, and defines the priorities addressed by a demonstrator program. It recalls the context created by the challenges of climate change and the excessive dependence on fossil fuels, and the objectives defined by the European Union in terms of biofuel share in the global consumption. Then, it describes the candidate technologies for the production of this second generation of biofuels, those based on thermo-chemical processes (mainly the pyrolysis-gasification of biomass), and those based on biochemical processes (enzymatic hydrolysis and fermentation of biomass). It highlights the technological challenges for these processes, and describes the various objectives of research projects supported by a 'demonstrator fund'. It discusses the necessity to develop demonstrators, and mentions some current projects of private companies, competitiveness clusters, and public research institutions. An agenda is defined from 2009 to 2020 which encompasses the research, demonstration and operation phases. An appendix provides brief presentations of thermo-chemical or biological demonstrators currently under operation or under construction in foreign countries (Germany, Finland, United States of America, Sweden, Spain, Canada, Japan, and Denmark)

  12. Bio-fuels are not so green

    International Nuclear Information System (INIS)

    Lemarchand, F.

    2007-01-01

    Today there is an unrelenting trend for bio-fuels but some scientists question their utility. Some surveys show that the environmental balance sheet for bio-fuels is strongly positive for instance it is assessed that the production of 1 MJ of ethanol from beet roots of wheat requires only 0.49 MJ of fossil energy, interesting figure when compared to the 1.14 MJ of fossil energy needed to produce 1 MJ of gasoline. Other studies are less optimistic, all depends strongly on the basic data used and on the approach followed. Some scientists wonder whether all the pollutants generated in the transformation processes are well taken into account. In fact the environment benefit of the first generation of bio-fuels is mild because scientists do not know how to use efficiently the wood-cellulose by-products of plants. There is a notably exception to that, it is the sugar cane in Brazil, this plant has a good energy conversion rate and its by-products are completely and efficiently used in industry. A way to valorize cellulose by-products is to transform them in ethanol and hydrogen through the use of mushroom enzymes. (A.C.)

  13. Application of Electroporation Technique in Biofuel Processing

    Directory of Open Access Journals (Sweden)

    Yousuf Abu

    2017-01-01

    Full Text Available Biofuels production is mostly oriented with fermentation process, which requires fermentable sugar as nutrient for microbial growth. Lignocellulosic biomass (LCB represents the most attractive, low-cost feedstock for biofuel production, it is now arousing great interest. The cellulose that is embedded in the lignin matrix has an insoluble, highly-crystalline structure, so it is difficult to hydrolyze into fermentable sugar or cell protein. On the other hand, microbial lipid has been studying as substitute of plant oils or animal fat to produce biodiesel. It is still a great challenge to extract maximum lipid from microbial cells (yeast, fungi, algae investing minimum energy.Electroporation (EP of LCB results a significant increase in cell conductivity and permeability caused due to the application of an external electric field. EP is required to alter the size and structure of the biomass, to reduce the cellulose crystallinity, and increase their porosity as well as chemical composition, so that the hydrolysis of the carbohydrate fraction to monomeric sugars can be achieved rapidly and with greater yields. Furthermore, EP has a great potential to disrupt the microbial cell walls within few seconds to bring out the intracellular materials (lipid to the solution. Therefore, this study aims to describe the challenges and prospect of application of EP technique in biofuels processing.

  14. Estimating Nitrogen Load Resulting from Biofuel Mandates

    Science.gov (United States)

    Alshawaf, Mohammad; Douglas, Ellen; Ricciardi, Karen

    2016-01-01

    The Energy Policy Act of 2005 and the Energy Independence and Security Act (EISA) of 2007 were enacted to reduce the U.S. dependency on foreign oil by increasing the use of biofuels. The increased demand for biofuels from corn and soybeans could result in an increase of nitrogen flux if not managed properly. The objectives of this study are to estimate nitrogen flux from energy crop production and to identify the catchment areas with high nitrogen flux. The results show that biofuel production can result in an increase of nitrogen flux to the northern Gulf of Mexico from 270 to 1742 thousand metric tons. Using all cellulosic (hay) ethanol or biodiesel to meet the 2022 mandate is expected to reduce nitrogen flux; however, it requires approximately 25% more land when compared to other scenarios. Producing ethanol from switchgrass rather than hay results in three-times more nitrogen flux, but requires 43% less land. Using corn ethanol for 2022 mandates is expected to have double the nitrogen flux when compared to the EISA-specified 2022 scenario; however, it will require less land area. Shifting the U.S. energy supply from foreign oil to the Midwest cannot occur without economic and environmental impacts, which could potentially lead to more eutrophication and hypoxia. PMID:27171101

  15. New Insights in Polymer-Biofuels Interaction

    Directory of Open Access Journals (Sweden)

    Richaud Emmanuel

    2015-02-01

    Full Text Available This paper deals with polymer-fuel interaction focusing on specific effects of biofuels on polyethylene (PE in automotive applications. The practical objective is to develop a predictable approach for durability of polyethylene tanks in contact of ethanol based or biofuel based fuels. In the case of ethanol, the main consequence on PE durability is a reduction of the rate of stabilizer extraction; this latter phenomenon can be modeled by first order kinetics with a rate constant that obeys the Arrhenius equation. Concerning biodiesels, the study was focused on soy and rapeseed methyl ester which were compared to methyl oleate and methyl linoleate used as model compounds. Here, PE-fuel interactions can be described as well as physical interaction, linked to the oil penetration into the polymer, as chemical interaction linked to an eventual co-oxidation of PE and oil. Both aspects were investigated. Concerning biofuel transport in PE, it appeared that the oil diffusivity depends only of temperature and oil molar mass. Some aspects of the temperature dependence of the oil solubility in PE are discussed. About chemical interaction between oil and PE, it was put in evidence that unsaturated fatty esters promote and accelerate PE oxidation. A co-oxidation kinetic model was proposed to describe this process.

  16. Developing symbiotic consortia for lignocellulosic biofuel production

    Energy Technology Data Exchange (ETDEWEB)

    Zuroff, Trevor R.; Curtis, Wayne R. [Pennsylvania State Univ., University Park, PA (United States). Dept. of Chemical Engineering

    2012-02-15

    The search for petroleum alternatives has motivated intense research into biological breakdown of lignocellulose to produce liquid fuels such as ethanol. Degradation of lignocellulose for biofuel production is a difficult process which is limited by, among other factors, the recalcitrance of lignocellulose and biological toxicity of the products. Consolidated bioprocessing has been suggested as an efficient and economical method of producing low value products from lignocellulose; however, it is not clear whether this would be accomplished more efficiently with a single organism or community of organisms. This review highlights examples of mixtures of microbes in the context of conceptual models for developing symbiotic consortia for biofuel production from lignocellulose. Engineering a symbiosis within consortia is a putative means of improving both process efficiency and stability relative to monoculture. Because microbes often interact and exist attached to surfaces, quorum sensing and biofilm formation are also discussed in terms of consortia development and stability. An engineered, symbiotic culture of multiple organisms may be a means of assembling a novel combination of metabolic capabilities that can efficiently produce biofuel from lignocellulose. (orig.)

  17. Biofuel Combustion Fly Ash Influence on the Properties of Concrete

    Directory of Open Access Journals (Sweden)

    Aurelijus Daugėla

    2016-02-01

    Full Text Available Cement as the binding agent in the production of concrete can be replaced with active mineral admixtures. Biofuel combustion fly ash is one of such admixtures. Materials used for the study: Portland cement CEM I 42.5 R, sand of 0/4 fraction, gravel of 4/16 fraction, biofuel fly ash, superplasticizer, water. Six compositions of concrete were designed by replacing 0%, 5%, 10%, 15% 20%, and 25% of cement with biofuel fly ash. The article analyses the effect of biofuel fly ash content on the properties of concrete. The tests revealed that the increase of biofuel fly ash content up to 20% increases concrete density and compressive strength after 7 and 28 days of curing and decreases water absorption, with corrected water content by using plasticizing admixture. It was found that concrete where 20% of cement is replaced by biofuel ash has higher frost resistance.

  18. Biofuels: stakes, perspectives and researches; Biocarburants: enjeux, perspectives et recherches

    Energy Technology Data Exchange (ETDEWEB)

    Appert, O.; Ballerin, D.; Montagne, X.

    2004-07-01

    The French institute of petroleum (IFP) is a major intervener of the biofuels sector, from the production to the end-use in engines. In this press conference, the IFP takes stock of the technological, environmental and economical stakes of today and future biofuel production processes and of their impact on transports. This document gathers 2 presentations dealing with: IFP's research strategy on biofuels (transparencies: context; today's processes: ethanol, ETBE, bio-diesel; tomorrows processes: biomass to liquid; perspectives), bio-diesel fuel: the Axens process selected by Diester Industrie company for its Sete site project of bio-diesel production unit. The researches carried out at the IFP on biofuels and biomass are summarized in an appendix: advantage and drawbacks of biofuels, the ethanol fuel industry, the bio-diesel industry, biomass to liquid fuels, French coordinated research program, statistical data of biofuel consumption in France, Spain and Germany. (J.S.)

  19. Engineering microbes for tolerance to next-generation biofuels

    Directory of Open Access Journals (Sweden)

    Dunlop Mary J

    2011-09-01

    Full Text Available Abstract A major challenge when using microorganisms to produce bulk chemicals such as biofuels is that the production targets are often toxic to cells. Many biofuels are known to reduce cell viability through damage to the cell membrane and interference with essential physiological processes. Therefore, cells must trade off biofuel production and survival, reducing potential yields. Recently, there have been several efforts towards engineering strains for biofuel tolerance. Promising methods include engineering biofuel export systems, heat shock proteins, membrane modifications, more general stress responses, and approaches that integrate multiple tolerance strategies. In addition, in situ recovery methods and media supplements can help to ease the burden of end-product toxicity and may be used in combination with genetic approaches. Recent advances in systems and synthetic biology provide a framework for tolerance engineering. This review highlights recent targeted approaches towards improving microbial tolerance to next-generation biofuels with a particular emphasis on strategies that will improve production.

  20. Fuel taxes and biofuel promotion: a complementary approach

    International Nuclear Information System (INIS)

    Santamaría, Marta; Azqueta, Diego

    2015-01-01

    Public support for renewable energy technologies is usually justified in terms of its contribution to reducing energy dependency; an improvement in environmental quality and a stimulation of economic activity and employment. In the case of biofuels, greenhouse gas emissions reduction has received significant attention. Nevertheless, nowadays there is a lively debate surrounding the convenience of biofuels. This is a consequence of the potentially negative impacts revealed from their production on a large scale. The aim of the present work is to analyses the potential contribution of biofuels to the main impact categories identified above. This paper tries to analyze the role of biofuel promotion in the context of fuel taxes. Based on the assessment of biofuels in Spain related to environmental damage and economic impacts, it shows that fuel taxes and biofuel promotion should be considered as complementary tools and treated accordingly. (full text)

  1. Microfluidics' great promise for Biology - Microfluidics as a new engine for the molecular sciences

    KAUST Repository

    Kodzius, Rimantas

    2010-06-04

    History of the Life Sciences Origins of life Discoveries and instrumentation The power of genetic variation Diagnostics based on DNA/ protein variation Genomic scanning providers DNA sequencing companies Microfluidics story Commercial products available P

  2. Development & Characterization of Multifunctional Microfluidic Materials

    Science.gov (United States)

    Ucar, Ahmet Burak

    The field of microfluidics has been mostly investigated for miniaturized lab on a chip devices for analytical and clinical applications. However, there is an emerging class of "smart" microfluidic materials, combining microfluidics with soft polymers to yield new functionalities. The best inspiration for such materials found in nature is skin, whose functions are maintained and controlled by a vascular "microfluidic" network. We report here the development and characterization of a few new classes of microfluidic materials. First, we introduced microfluidic materials that can change their stiffness on demand. These materials were based on an engineered microchannel network embedded into a matrix of polydimethylsiloxane (PDMS), whose channels were filled with a liquid photoresist (SU- 8). The elastomer filled with the photoresist was initially soft. The materials were shaped into a desired geometry and then exposed to UV-light. Once photocured, the material preserved the defined shape and it could be bent, twisted or stretched with a very high recoverable strain. As soon as the external force was removed the material returned back to its predefined shape. Thus, the polymerized SU-8 acted as the 'endoskeleton' of the microfluidic network, which drastically increased the composite's elastic and bending moduli. Second, we demonstrated a class of simple and versatile soft microfluidic materials that can be turned optically transparent or colored on demand. These materials were made in the form of flexible sheets containing a microchannel network embedded in PDMS, similar to the photocurable materials. However, this time the channels were filled with a glycerolwater mixture, whose refractive index was matched with that of the PDMS matrix. By pumping such dye solutions into the channel network and consecutively replacing the medium, we showed that we can control the material's color and light transmittance in the visible and near-infrared regions, which can be used for

  3. Increase of food commodities prices and their relationship with biofuels

    International Nuclear Information System (INIS)

    Ortiz-Alvarez, Marianela; Piloto-Rodríguez, Ramón

    2017-01-01

    Biofuels are without any doubt, an alternative to the actual energy matrix. In this work, through the analysis of the main influencing factors in the increase of food commodities prices, is demonstrated that this phenomena is not exclusive due to biofuels production. Comparing the food commodities prices with biofuels production and petroleum prices respectively, a stronger correlation between food and petroleum prices was observed, demonstrating the strong influence of the conventional energy market on agricultural products. (author)

  4. Bio-fuels barometer - EurObserv'ER - July 2016

    International Nuclear Information System (INIS)

    2016-07-01

    The European bio-fuel market is now regulated by the directive, known as ILUC, whose wording focuses on the environmental impact of first generation bio-fuel development. This long-awaited clarification has arrived against the backdrop of falling oil prices and shrinking European Union bio-fuel consumption, which should drop by 1.7% between 2014 and 2015, according to EurObserv'ER

  5. Determination of calorific values of some renewable biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, Jothi V.; Pratt, Benjamin C. [Department of Chemistry, North Carolina A and T State University, Greensboro, North Carolina (United States)

    1996-06-01

    Thermal methods such as differential scanning calorimetry (DSC), and elemental analysis (EA) were employed to determine the calorific values of some renewable biofuels either directly or indirectly. The biofuels tested were the common milkweed, dogbane, kudzu, and eucalyptus tree. The purpose of this work was to optimize the experimental conditions for DSC analysis of biofuels, improve the calorific values by adding metal oxides as catalysts, and compare the heat values between DSC and EA analyses

  6. Recommendations for a sustainable development of biofuels in France

    International Nuclear Information System (INIS)

    Douaud, A.; Gruson, J.F.

    2006-01-01

    The biofuels are presented as a solution to the greenhouse gases and the petroleum consumption decrease. The development of the biofuels needs an active research of the production, transformation and use costs improvement. It will be necessary to prepare the market of the biofuels to the globalization. Some recommendations are also provided in the domains of the vegetal oil ester, the ethanol for the diesel and for the development of simulation tools to evaluate the costs. (A.L.B.)

  7. Current status: biomass valorisation and biofuels in Singapore

    International Nuclear Information System (INIS)

    Guermont, C.; Barbi, A.P.

    2010-05-01

    After having briefly presented the main types of biofuels (bio-ethanol, bio-diesel) and their first, second and third generation technologies to produce them (from food crops, from non food crops, and from algae), this report presents Singapore public R and D centres working in the field of biofuels development, and their activities. It also presents actors belonging to the private sector, and various realized and announced projects on biofuels

  8. Advice on the accelerated market implementation of advanced biofuels

    International Nuclear Information System (INIS)

    2008-04-01

    The Platform for Sustainable Mobility aims to promote the accelerated market introduction of more sustainable motor fuels and vehicle technology. The Platform distinguishes four transition paths: hybridization of the fleet of cars; implementation of biofuels; hydrogen-fuelled driving (driving on natural gas and biogas); intelligent transport systems (ITS). This advice involves part of the transition path for the implementation of biofuels, i.e. accelerated market introduction of advances biofuels. [mk] [nl

  9. 2016 National Algal Biofuels Technology Review Fact Sheet

    Energy Technology Data Exchange (ETDEWEB)

    None

    2016-06-01

    Algae-based biofuels and bioproducts offer great promise in contributing to the U.S. Department of Energy (DOE) Bioenergy Technologies Office’s (BETO’s) vision of a thriving and sustainable bioeconomy fueled by innovative technologies. The state of technology for producing algal biofuels continues to mature with ongoing investment by DOE and the private sector, but additional research, development, and demonstration (RD&D) is needed to achieve widespread deployment of affordable, scalable, and sustainable algal biofuels.

  10. Innovative technological paradigm-based approach towards biofuel feedstock

    International Nuclear Information System (INIS)

    Xu, Jiuping; Li, Meihui

    2017-01-01

    Highlights: • DAS was developed through an innovative approach towards literature mining and technological paradigm theory. • A novel concept of biofuel feedstock development paradigm (BFDP) is proposed. • The biofuel production diffusion velocity model gives predictions for the future. • Soft path appears to be the driving force for the new paradigm shift. • An integrated biofuel production feedstock system is expected to play a significant role in a low-carbon sustainable future. - Abstract: Biofuels produced from renewable energy biomass are playing a more significant role because of the environmental problems resulting from the use of fossil fuels. However, a major problem with biofuel production is that despite the range of feedstock that can be used, raw material availability varies considerably. By combining a series of theories and methods, the research objective of this study is to determine the current developments and the future trends in biofuel feedstock. By combining technological paradigm theory with literature mining, it was found that biofuel feedstock production development followed a three-stage trajectory, which was in accordance with the traditional technological paradigm – the S-curve. This new curve can be divided into BFDP (biofuel feedstock development paradigm) competition, BFDP diffusion, and BFDP shift. The biofuel production diffusion velocity model showed that there has been constant growth from 2000, with the growth rate reaching a peak in 2008, after which time it began to drop. Biofuel production worldwide is expected to remain unchanged until 2030 when a paradigm shift is expected. This study also illustrates the results of our innovative procedure – a combination of the data analysis system and the technological paradigm theory – for the present biofuel feedstock soft path that will lead to this paradigm shift, with integrated biofuel production feedstock systems expected to be a significant new trend.

  11. Carbon and environmental footprinting of global biofuel production

    OpenAIRE

    Hammond, Geoff P.; Seth, S.M.

    2013-01-01

    The carbon and environmental footprints associated with the global production of biofuels have been computed from a baseline of 2007-2009 out until 2019. Estimates of future global biofuel production were adopted from OECD-FAO and related projections. In order to determine the footprints associated with these (essentially 'first generation') biofuel resources, the overall environmental footprint was disaggregated into bioproductive land, built land, carbon, embodied energy, materials and wast...

  12. Livelihood implications of biofuel crop production: Implications for governance

    DEFF Research Database (Denmark)

    Hunsberger, Carol; Bolwig, Simon; Corbera, Esteve

    2014-01-01

    While much attention has focused on the climate change mitigation potential of biofuels, research from the social sciences increasingly highlights the social and livelihood impacts of their expanded production. Policy and governance measures aimed at improving the social effects of biofuels have...... by their cultivation in the global South – income, food security, access to land-based resources, and social assets – revealing that distributional effects are crucial to evaluating the outcomes of biofuel production across these dimensions. Second, we ask how well selected biofuel governance mechanisms address...

  13. Synthetic biology for microbial production of lipid-based biofuels.

    Science.gov (United States)

    d'Espaux, Leo; Mendez-Perez, Daniel; Li, Rachel; Keasling, Jay D

    2015-12-01

    The risks of maintaining current CO2 emission trends have led to interest in producing biofuels using engineered microbes. Microbial biofuels reduce emissions because CO2 produced by fuel combustion is offset by CO2 captured by growing biomass, which is later used as feedstock for biofuel fermentation. Hydrocarbons found in petroleum fuels share striking similarity with biological lipids. Here we review synthetic metabolic pathways based on fatty acid and isoprenoid metabolism to produce alkanes and other molecules suitable as biofuels. We further discuss engineering strategies to optimize engineered biosynthetic routes, as well as the potential of synthetic biology for sustainable manufacturing. Published by Elsevier Ltd.

  14. [Progress in synthesis technologies and application of aviation biofuels].

    Science.gov (United States)

    Sun, Xiaoying; Liu, Xiang; Zhao, Xuebing; Yang, Ming; Liu, Dehua

    2013-03-01

    Development of aviation biofuels has attracted great attention worldwide because that the shortage of fossil resources has become more and more serious. In the present paper, the development background, synthesis technologies, current application status and existing problems of aviation biofuels were reviewed. Several preparation routes of aviation biofuels were described, including Fischer-Tropsch process, catalytic hydrogenation and catalytic cracking of bio-oil. The status of flight tests and commercial operation were also introduced. Finally the problems for development and application of aviation biofuels were stated, and some accommodation were proposed.

  15. Tools and methodologies to support more sustainable biofuel feedstock production.

    Science.gov (United States)

    Dragisic, Christine; Ashkenazi, Erica; Bede, Lucio; Honzák, Miroslav; Killeen, Tim; Paglia, Adriano; Semroc, Bambi; Savy, Conrad

    2011-02-01

    Increasingly, government regulations, voluntary standards, and company guidelines require that biofuel production complies with sustainability criteria. For some stakeholders, however, compliance with these criteria may seem complex, costly, or unfeasible. What existing tools, then, might facilitate compliance with a variety of biofuel-related sustainability criteria? This paper presents four existing tools and methodologies that can help stakeholders assess (and mitigate) potential risks associated with feedstock production, and can thus facilitate compliance with requirements under different requirement systems. These include the Integrated Biodiversity Assessment Tool (IBAT), the ARtificial Intelligence for Ecosystem Services (ARIES) tool, the Responsible Cultivation Areas (RCA) methodology, and the related Biofuels + Forest Carbon (Biofuel + FC) methodology.

  16. From biomass to sustainable biofuels in southern Africa

    Energy Technology Data Exchange (ETDEWEB)

    Van Zyl, W.H.; Den Haan, R.; Rose, S.H.; La Grange, D.C.; Bloom, M. [Stellenbosch Univ., Matieland (South Africa). Dept. of Microbiology; Gorgens, J.F.; Knoetze, J.H. [Stellenbosch Univ., Matieland (South Africa). Dept. of Process Engineering; Von Blottnitz, H. [Cape Town Univ., Rondebosch (South Africa). Dept. of Chemical Engineering

    2009-07-01

    This presentation reported on a global sustainable bioenergy project with particular reference to South Africa's strategy to develop biofuels. The current biofuel production in South Africa was presented along with the potential for biofuels production and other clean alternative fuels. The South African industrial biofuel strategy (IBS) was developed in 2007 with a mandate to create jobs in the energy-crop and biofuels value chain; attract investment into rural areas; promote agricultural development; and reduce the import of foreign oil. The proposed crops for bioethanol include sugar cane and sugar beet, while the proposed crops for biodiesel include sunflower, canola and soya beans. The exclusion of maize was based on food security concerns. Jatropha curcas was also excluded because it is considered to be an invasive species. In addition to environmental benefits, the production of biofuels from biomass in Africa offers improved energy security, economic development and social upliftment. All biofuel projects are evaluated to ensure that these benefits are realized. Although first generation technologies do not score well due to marginal energy balance, negative life cycle impacts or detriment to biodiversity, the conversion of lignocellulosic biomass scores well in terms of enabling the commercialization of second generation biofuels. This paper discussed both the biochemical and thermochemical technological interventions needed to develop commercially-viable second generation lignocellulose conversion technologies to biofuels. tabs., figs.

  17. Biofuels barometer - EurObserv'ER - July 2015

    International Nuclear Information System (INIS)

    2015-07-01

    +6.1% The increase of biofuel consumption in European Union transport between 2013 and 2014 (in energy content). Biofuel consumption for transport picked up in Europe after a year of uncertainty and decline, increasing by 6.1% over 2013, to 14 million toe (Mtoe) according to EurObserv'ER's first estimates. However it is still below its 2012 level when 14.5 Mtoe of biofuel was incorporated. Consumption of biofuel that meets the European Renewable Energy directive's sustainability criteria rose to 12.5 Mtoe, its highest level so far

  18. Energy properties of solid fossil fuels and solid biofuels

    International Nuclear Information System (INIS)

    Holubcik, Michal; Jandacka, Jozef; Kolkova, Zuzana

    2016-01-01

    The paper deals about the problematic of energy properties of solid biofuels in comparison with solid fossil fuels. Biofuels are alternative to fossil fuels and their properties are very similar. During the experiments were done in detail experiments to obtain various properties of spruce wood pellets and wheat straw pellets like biofuels in comparison with brown coal and black coal like fossil fuels. There were tested moisture content, volatile content, fixed carbon content, ash content, elementary analysis (C, H, N, S content) and ash fusion temperatures. The results show that biofuels have some advantages and also disadvantages in comparison with solid fossil fuels.

  19. Energy properties of solid fossil fuels and solid biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Holubcik, Michal, E-mail: michal.holubcik@fstroj.uniza.sk; Jandacka, Jozef, E-mail: jozef.jandacka@fstroj.uniza.sk [University of Žilina, Faculty of Mechanical Engineering, Department of Power Engineering, Univerzitná 8215/1, 010 26 Žilina (Slovakia); Kolkova, Zuzana, E-mail: zuzana.kolkova@rc.uniza.sk [Research centre, University of Žilina, Univerzitna 8215/1, 010 26 Žilina (Slovakia)

    2016-06-30

    The paper deals about the problematic of energy properties of solid biofuels in comparison with solid fossil fuels. Biofuels are alternative to fossil fuels and their properties are very similar. During the experiments were done in detail experiments to obtain various properties of spruce wood pellets and wheat straw pellets like biofuels in comparison with brown coal and black coal like fossil fuels. There were tested moisture content, volatile content, fixed carbon content, ash content, elementary analysis (C, H, N, S content) and ash fusion temperatures. The results show that biofuels have some advantages and also disadvantages in comparison with solid fossil fuels.

  20. Improving the environmental performance of biofuels with industrial symbiosis

    International Nuclear Information System (INIS)

    Martin, Michael; Eklund, Mats

    2011-01-01

    In the production of biofuels for transport many critics have argued about the poor energy efficiency and environmental performance of the production industries. Optimism is thus set on the production of second generation biofuels, while first generation biofuels continue to dominate worldwide. Therefore it is interesting to consider how the environmental performance of first generation biofuel industries can be improved. The field of industrial symbiosis offers many possibilities for potential improvements in the biofuel industry and theories from this research field are used in this paper to highlight how environmental performance improvements can be accomplished. This comes in the form of by-product synergies and utility synergies which can improve material and energy handling. Furthermore, the processes and products can gain increased environmental performance improvements by the adaption of a renewable energy system which will act as a utility provider for many industries in a symbiotic network. By-products may thereafter be upcycled through biogas production processes to generate both energy and a bio-fertilizer. A case study of an actual biofuel industrial symbiosis is also reviewed to provide support for these theories. -- Highlights: → By-product and utility synergies may improve the production processes of biofuel industries for reduced energy consumption and improved environmental performance. → Upcycling tenants can make use of wastes to upgrade waste to a valuable product and/or energy source. → Energy systems for biofuel production have a large influence on the performance of biofuel industries.

  1. Will biofuel projects in Southeast Asia become white elephants?

    International Nuclear Information System (INIS)

    Goh, Chun Sheng; Lee, Keat Teong

    2010-01-01

    Southeast Asia's attempt to join the global biofuel development has not been very successful, despite the large amount of subsidies and incentives allotted for biofuel projects. The outcome of these projects has failed to meet expectation due to overrated assumptions and shortsighted policies. Utilization of edible feedstock such as palm oil and sugar cane for biofuel has disrupted the fragile industry due to the fluctuations of feedstock prices. The appropriate research on jatropha to prove its economic and environmental feasibility as energy crop has not been performed. Biofuel development in Southeast Asia remains at an early stage of development and requires highly intensive monitoring and strict legal enforcement to ensure future success.

  2. The development of the biofuels in the french farms

    International Nuclear Information System (INIS)

    Treguer, D.; Sourie, J.C.

    2005-03-01

    At first, developed to compensate the farmers incomes after 1993, the biofuels are going today on a second development phase, in the framework of the Kyoto protocol. The aim of this paper is to define the particularities of the biofuels production agricultural phase. The most important aspects of the common agricultural policy (PAC) for the biofuels are underlined. The costs of the raw material and the tool developed by the INRA to estimate the biofuels costs are also presented. In conclusion the authors propose some reference results. (A.L.B.)

  3. Biofuel Sustainability and the Formation of Transnational Hybrid Governance

    DEFF Research Database (Denmark)

    Ponte, Stefano; Daugbjerg, Carsten

    2015-01-01

    We examine the transnational governance of biofuel sustainability and its coexistence with the WTO trade regime. The way in which the EU Renewable Energy Directive (RED) is shaping transnational biofuel governance shows deep and mutual dependence between public and private. The EU relies on a pri......We examine the transnational governance of biofuel sustainability and its coexistence with the WTO trade regime. The way in which the EU Renewable Energy Directive (RED) is shaping transnational biofuel governance shows deep and mutual dependence between public and private. The EU relies...

  4. Synthetic biology for microbial production of lipid-based biofuels

    Energy Technology Data Exchange (ETDEWEB)

    d' Espaux, L; Mendez-Perez, D; Li, R; Keasling, JD

    2015-10-23

    The risks of maintaining current CO2 emission trends have led to interest in producing biofuels using engineered microbes. Microbial biofuels reduce emissions because CO2 produced by fuel combustion is offset by CO2 captured by growing biomass, which is later used as feedstock for biofuel fermentation. Hydrocarbons found in petroleum fuels share striking similarity with biological lipids. Here in this paper we review synthetic metabolic pathways based on fatty acid and isoprenoid metabolism to produce alkanes and other molecules suitable as biofuels. Lastly, we further discuss engineering strategies to optimize engineered biosynthetic routes, as well as the potential of synthetic biology for sustainable manufacturing.

  5. Microfluidics for Antibiotic Susceptibility and Toxicity Testing

    Directory of Open Access Journals (Sweden)

    Jing Dai

    2016-10-01

    Full Text Available The recent emergence of antimicrobial resistance has become a major concern for worldwide policy makers as very few new antibiotics have been developed in the last twenty-five years. To prevent the death of millions of people worldwide, there is an urgent need for a cheap, fast and accurate set of tools and techniques that can help to discover and develop new antimicrobial drugs. In the past decade, microfluidic platforms have emerged as potential systems for conducting pharmacological studies. Recent studies have demonstrated that microfluidic platforms can perform rapid antibiotic susceptibility tests to evaluate antimicrobial drugs’ efficacy. In addition, the development of cell-on-a-chip and organ-on-a-chip platforms have enabled the early drug testing, providing more accurate insights into conventional cell cultures on the drug pharmacokinetics and toxicity, at the early and cheaper stage of drug development, i.e., prior to animal and human testing. In this review, we focus on the recent developments of microfluidic platforms for rapid antibiotics susceptibility testing, investigating bacterial persistence and non-growing but metabolically active (NGMA bacteria, evaluating antibiotic effectiveness on biofilms and combinatorial effect of antibiotics, as well as microfluidic platforms that can be used for in vitro antibiotic toxicity testing.

  6. Droplet microfluidics in (bio) chemical analysis

    Czech Academy of Sciences Publication Activity Database

    Basova, E. Y.; Foret, František

    2015-01-01

    Roč. 140, č. 1 (2015), s. 22-38 ISSN 0003-2654 R&D Projects: GA ČR(CZ) GBP206/12/G014 Institutional support: RVO:68081715 Keywords : droplet chemistry * bio analysis * microfluidics * protein Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 4.033, year: 2015

  7. Light-responsive polymers for microfluidic applications

    NARCIS (Netherlands)

    ter Schiphorst, J.; Saez, J.; Diamond, D.; Benito-Lopez, F.; Schenning, A.P.H.J.

    2018-01-01

    While the microfluidic device itself may be small, often the equipment required to control fluidics in the chip unit is large e.g. pumps, valves and mixing units, which can severely limit practical use and functional scalability. In addition, components associated with fluidic control of the device,

  8. Droplet microfluidics in (bio) chemical analysis

    Czech Academy of Sciences Publication Activity Database

    Basova, E. Y.; Foret, František

    2015-01-01

    Roč. 140, č. 1 (2015), s. 22-38 ISSN 0003-2654 R&D Projects: GA ČR(CZ) GBP206/12/G014 Institutional support: RVO:68081715 Keywords : droplet chemistry * bioanalysis * microfluidics * protein Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 4.033, year: 2015

  9. A Centrifugal Microfluidic Platform Using SLM Extraction

    DEFF Research Database (Denmark)

    Andreasen, Sune Zoëga; Burger, Robert; Emnéus, Jenny

    2016-01-01

    Here we present a pump-less microfluidic pla>orm which performs sample clean-up and enrichment in a single step, by integraAng Supported Liquid Membrane (SLM) extracAon. Our pla>orm offers a simple, yet very efficient, method for achieving sample pre-treatment and enrichment of rare analytes, in ...

  10. Microfluidic desalination techniques and their potential applications

    NARCIS (Netherlands)

    Roelofs, Susan Helena; van den Berg, Albert; Odijk, Mathieu

    2015-01-01

    In this review we discuss recent developments in the emerging research field of miniaturized desalination. Traditionally desalination is performed to convert salt water into potable water and research is focused on improving performance of large-scale desalination plants. Microfluidic desalination

  11. Micromechanical photothermal analyser of microfluidic samples

    DEFF Research Database (Denmark)

    2014-01-01

    The present invention relates to a micromechanical photothermal analyser of microfluidic samples comprising an oblong micro-channel extending longitudinally from a support element, the micro-channel is made from at least two materials with different thermal expansion coefficients, wherein...

  12. Design of microfluidic bioreactors using topology optimization

    DEFF Research Database (Denmark)

    Okkels, Fridolin; Bruus, Henrik

    2007-01-01

    We address the design of optimal reactors for supporting biological cultures using the method of topology optimization. For some years this method have been used to design various optimal microfluidic devices.1-4 We apply this method to distribute optimally biologic cultures within a flow...

  13. Microfluidic Sensing Platforms for Medicine and Diagnostics

    DEFF Research Database (Denmark)

    Kiilerich-Pedersen, Katrine

    the specialized laboratory. Microfluidic cell migration devices, imitating in vivo conditions were developed with success, improving the in vitro experimental setup for basic research and drug discovery. Polymer biosensors have reached a new level of maturity, and pathogen detection could benefit from...

  14. Biocatalytic process development using microfluidic miniaturized systems

    DEFF Research Database (Denmark)

    Krühne, Ulrich; Heintz, Søren; Ringborg, Rolf Hoffmeyer

    2014-01-01

    The increasing interest in biocatalytic processes means there is a clear need for a new systematic development paradigm which encompasses both protein engineering and process engineering. This paper argues that through the use of a new microfluidic platform, data can be collected more rapidly...

  15. Droplet Manipulations in Two Phase Flow Microfluidics

    NARCIS (Netherlands)

    Pit, Arjen; Duits, Michael H.G.; Mugele, Friedrich Gunther

    2015-01-01

    Even though droplet microfluidics has been developed since the early 1980s, the number of applications that have resulted in commercial products is still relatively small. This is partly due to an ongoing maturation and integration of existing methods, but possibly also because of the emergence of

  16. Parallel single-cell analysis microfluidic platform

    NARCIS (Netherlands)

    van den Brink, Floris Teunis Gerardus; Gool, Elmar; Frimat, Jean-Philippe; Bomer, Johan G.; van den Berg, Albert; le Gac, Severine

    2011-01-01

    We report a PDMS microfluidic platform for parallel single-cell analysis (PaSCAl) as a powerful tool to decipher the heterogeneity found in cell populations. Cells are trapped individually in dedicated pockets, and thereafter, a number of invasive or non-invasive analysis schemes are performed.

  17. Review of Recent Metamaterial Microfluidic Sensors.

    Science.gov (United States)

    Salim, Ahmed; Lim, Sungjoon

    2018-01-15

    Metamaterial elements/arrays exhibit a sensitive response to fluids yet with a small footprint, therefore, they have been an attractive choice to realize various sensing devices when integrated with microfluidic technology. Micro-channels made from inexpensive biocompatible materials avoid any contamination from environment and require only microliter-nanoliter sample for sensing. Simple design, easy fabrication process, light weight prototype, and instant measurements are advantages as compared to conventional (optical, electrochemical and biological) sensing systems. Inkjet-printed flexible sensors find their utilization in rapidly growing wearable electronics and health-monitoring flexible devices. Adequate sensitivity and repeatability of these low profile microfluidic sensors make them a potential candidate for point-of-care testing which novice patients can use reliably. Aside from degraded sensitivity and lack of selectivity in all practical microwave chemical sensors, they require an instrument, such as vector network analyzer for measurements and not readily available as a self-sustained portable sensor. This review article presents state-of-the-art metamaterial inspired microfluidic bio/chemical sensors (passive devices ranging from gigahertz to terahertz range) with an emphasis on metamaterial sensing circuit and microfluidic detection. We also highlight challenges and strategies to cope these issues which set future directions.

  18. Review of Recent Metamaterial Microfluidic Sensors

    Directory of Open Access Journals (Sweden)

    Ahmed Salim

    2018-01-01

    Full Text Available Metamaterial elements/arrays exhibit a sensitive response to fluids yet with a small footprint, therefore, they have been an attractive choice to realize various sensing devices when integrated with microfluidic technology. Micro-channels made from inexpensive biocompatible materials avoid any contamination from environment and require only microliter–nanoliter sample for sensing. Simple design, easy fabrication process, light weight prototype, and instant measurements are advantages as compared to conventional (optical, electrochemical and biological sensing systems. Inkjet-printed flexible sensors find their utilization in rapidly growing wearable electronics and health-monitoring flexible devices. Adequate sensitivity and repeatability of these low profile microfluidic sensors make them a potential candidate for point-of-care testing which novice patients can use reliably. Aside from degraded sensitivity and lack of selectivity in all practical microwave chemical sensors, they require an instrument, such as vector network analyzer for measurements and not readily available as a self-sustained portable sensor. This review article presents state-of-the-art metamaterial inspired microfluidic bio/chemical sensors (passive devices ranging from gigahertz to terahertz range with an emphasis on metamaterial sensing circuit and microfluidic detection. We also highlight challenges and strategies to cope these issues which set future directions.

  19. Reaction and separation opportunities with microfluidic devices

    NARCIS (Netherlands)

    Kolfschoten, R.C.

    2011-01-01

    Microfluidic devices make precisely controlled processing of substances possible on a microliter level. The advantage is that, due to the small sizes, the driving forces for mass and heat transfer are high. The surface to volume ratios are also high, which can benefit many surface oriented

  20. High content screening in microfluidic devices

    Science.gov (United States)

    Cheong, Raymond; Paliwal, Saurabh; Levchenko, Andre

    2011-01-01

    Importance of the field Miniaturization is key to advancing the state-of-the-art in high content screening (HCS), in order to enable dramatic cost savings through reduced usage of expensive biochemical reagents and to enable large-scale screening on primary cells. Microfluidic technology offers the potential to enable HCS to be performed with an unprecedented degree of miniaturization. Areas covered in this review This perspective highlights a real-world example from the authors’ work of HCS assays implemented in a highly miniaturized microfluidic format. Advantages of this technology are discussed, including cost savings, high throughput screening on primary cells, improved accuracy, the ability to study complex time-varying stimuli, and ease of automation, integration, and scaling. What the reader will gain The reader will understand the capabilities of a new microfluidics-based platform for HCS, and the advantages it provides over conventional plate-based HCS. Take home message Microfluidics technology will drive significant advancements and broader usage and applicability of HCS in drug discovery. PMID:21852997

  1. Wax-bonding 3D microfluidic chips

    KAUST Repository

    Gong, Xiuqing; Yi, Xin; Xiao, Kang; Li, Shunbo; Kodzius, Rimantas; Qin, Jianhua; Wen, Weijia

    2013-01-01

    We report a simple, low-cost and detachable microfluidic chip incorporating easily accessible paper, glass slides or other polymer films as the chip materials along with adhesive wax as the recycling bonding material. We use a laser to cut through the paper or film to form patterns and then sandwich the paper and film between glass sheets or polymer membranes . The hot-melt adhesive wax can realize bridge bonding between various materials, for example, paper, polymethylmethacrylate (PMMA) film, glass sheets, or metal plate. The bonding process is reversible and the wax is reusable through a melting and cooling process. With this process, a three-dimensional (3D) microfluidic chip is achievable by vacuating and venting the chip in a hot-water bath. To study the biocompatibility and applicability of the wax-based microfluidic chip, we tested the PCR compatibility with the chip materials first. Then we applied the wax-paper based microfluidic chip to HeLa cell electroporation (EP ). Subsequently, a prototype of a 5-layer 3D chip was fabricated by multilayer wax bonding. To check the sealing ability and the durability of the chip, green fluorescence protein (GFP) recombinant Escherichia coli (E. coli) bacteria were cultured, with which the chemotaxis of E. coli was studied in order to determine the influence of antibiotic ciprofloxacin concentration on the E. coli migration.

  2. Wax-bonding 3D microfluidic chips

    KAUST Repository

    Gong, Xiuqing

    2013-10-10

    We report a simple, low-cost and detachable microfluidic chip incorporating easily accessible paper, glass slides or other polymer films as the chip materials along with adhesive wax as the recycling bonding material. We use a laser to cut through the paper or film to form patterns and then sandwich the paper and film between glass sheets or polymer membranes . The hot-melt adhesive wax can realize bridge bonding between various materials, for example, paper, polymethylmethacrylate (PMMA) film, glass sheets, or metal plate. The bonding process is reversible and the wax is reusable through a melting and cooling process. With this process, a three-dimensional (3D) microfluidic chip is achievable by vacuating and venting the chip in a hot-water bath. To study the biocompatibility and applicability of the wax-based microfluidic chip, we tested the PCR compatibility with the chip materials first. Then we applied the wax-paper based microfluidic chip to HeLa cell electroporation (EP ). Subsequently, a prototype of a 5-layer 3D chip was fabricated by multilayer wax bonding. To check the sealing ability and the durability of the chip, green fluorescence protein (GFP) recombinant Escherichia coli (E. coli) bacteria were cultured, with which the chemotaxis of E. coli was studied in order to determine the influence of antibiotic ciprofloxacin concentration on the E. coli migration.

  3. Porous Microfluidic Devices - Fabrication adn Applications

    NARCIS (Netherlands)

    de Jong, J.; Geerken, M.J.; Lammertink, Rob G.H.; Wessling, Matthias

    2007-01-01

    The major part of microfluidic devices nowadays consists of a dense material that defines the fluidic structure. A generic fabrication method enabling the production of completely porous micro devices with user-defined channel networks is developed. The channel walls can be used as a (selective)

  4. Recent Advances in Magnetic Microfluidic Biosensors

    Directory of Open Access Journals (Sweden)

    Ioanna Giouroudi

    2017-07-01

    Full Text Available The development of portable biosening devices for the detection of biological entities such as biomolecules, pathogens, and cells has become extremely significant over the past years. Scientific research, driven by the promise for miniaturization and integration of complex laboratory equipment on inexpensive, reliable, and accurate devices, has successfully shifted several analytical and diagnostic methods to the submillimeter scale. The miniaturization process was made possible with the birth of microfluidics, a technology that could confine, manipulate, and mix very small volumes of liquids on devices integrated on standard silicon technology chips. Such devices are then directly translating the presence of these entities into an electronic signal that can be read out with a portable instrumentation. For the aforementioned tasks, the use of magnetic markers (magnetic particles—MPs—functionalized with ligands in combination with the application of magnetic fields is being strongly investigated by research groups worldwide. The greatest merits of using magnetic fields are that they can be applied either externally or from integrated microconductors and they can be well-tuned by adjusting the applied current on the microconductors. Moreover, the magnetic markers can be manipulated inside microfluidic channels by high gradient magnetic fields that can in turn be detected by magnetic sensors. All the above make this technology an ideal candidate for the development of such microfluidic biosensors. In this review, focus is given only to very recent advances in biosensors that use microfluidics in combination with magnetic sensors and magnetic markers/nanoparticles.

  5. Differential white cell count by centrifugal microfluidics.

    Energy Technology Data Exchange (ETDEWEB)

    Sommer, Gregory Jon; Tentori, Augusto M.; Schaff, Ulrich Y.

    2010-07-01

    We present a method for counting white blood cells that is uniquely compatible with centrifugation based microfluidics. Blood is deposited on top of one or more layers of density media within a microfluidic disk. Spinning the disk causes the cell populations within whole blood to settle through the media, reaching an equilibrium based on the density of each cell type. Separation and fluorescence measurement of cell types stained with a DNA dye is demonstrated using this technique. The integrated signal from bands of fluorescent microspheres is shown to be proportional to their initial concentration in suspension. Among the current generation of medical diagnostics are devices based on the principle of centrifuging a CD sized disk functionalized with microfluidics. These portable 'lab on a disk' devices are capable of conducting multiple assays directly from a blood sample, embodied by platforms developed by Gyros, Samsung, and Abaxis. [1,2] However, no centrifugal platform to date includes a differential white blood cell count, which is an important metric complimentary to diagnostic assays. Measuring the differential white blood cell count (the relative fraction of granulocytes, lymphocytes, and monocytes) is a standard medical diagnostic technique useful for identifying sepsis, leukemia, AIDS, radiation exposure, and a host of other conditions that affect the immune system. Several methods exist for measuring the relative white blood cell count including flow cytometry, electrical impedance, and visual identification from a stained drop of blood under a microscope. However, none of these methods is easily incorporated into a centrifugal microfluidic diagnostic platform.

  6. Methodological aspects on international biofuels trade: International streams and trade of solid and liquid biofuels in Finland

    International Nuclear Information System (INIS)

    Heinimoe, J.

    2008-01-01

    The use of biomass for fuel is increasing in industrialised countries. Rapidly developing biomass markets for energy purposes along with weak information on biofuels trade that statistics offer have been incentives for several recently published studies investigating the status of biofuels trade. The comparison of the studies is often challenging due particularly to the various approaches to the indirect trade of biofuels and the diverse data sources utilised. The purpose of this study was to provide an overview of the Finnish situation with respect to the status of the streams of international biofuels trade. Parallel to this, the study aimed to identify methodological and statistical challenges in observing international biofuels trade. The study analysed available statistical information and introduced a procedure to obtain a clear overview on import and export streams of biofuels. In Finland, the total direct import and export of biofuels, being mainly composed of wood pellets and tall oil, is tiny in comparison with the total consumption of biofuels. Instead, the indirect trade has remarkable importance. Large import volumes of industrial raw wood make Finland a net importer of biofuels. In 2004, approximately 22% (64 PJ) of wood-based energy in Finland originated from imported wood. The study showed that the indirect trade of biofuels may be a significant sector of global biofuels trade. In the case of Finland, a comprehensive compilation of statistics on energy and forestry enabled the determination of the trade status satisfactory. However, national and international statistics should be further developed to take better into consideration international trade and to support continuously developing biofuels markets. (author)

  7. Recent Inventions and Trends in Algal Biofuels Research.

    Science.gov (United States)

    Karemore, Ankush; Nayak, Manoranjan; Sen, Ramkrishna

    2016-01-01

    In recent times, when energy crisis compounded by global warming and climate change is receiving worldwide attention, the emergence of algae, as a better feedstock for third-generation biofuels than energy crops or plants, holds great promise. As compared to conventional biofuels feedstocks, algae offer several advantages and can alone produce a significant amount of biofuels sustainably in a shorter period to fulfill the rising demand for energy. Towards commercialisation, there have been numerous efforts put for- ward for the development of algae-derived biofuel. This article reviews and summarizes the recent inventions and the current trends that are reported and captured in relevant patents pertaining to the novel methods of algae biomass cultivation and processing for biofuels and value-added products. In addition, the recent advancement in techniques and technologies for microalgal biofuel production has been highlighted. Various steps involved in the production of algal biofuels have been considered in this article. Moreover, the work that advances to improve the efficiency and cost-effectiveness of the processes for the manufacture of biofuels has been presented. Our survey was conducted in the patent databases: WIPO, Spacenet and USPTO. There are still some technological bottlenecks that could be overcome by designing advanced photobioreactor and raceway ponds, developing new and low cost technologies for biomass cultivation, harvesting, drying and extraction. Recent advancement in algae biofuels methods is directed toward developing efficient and integrated systems to produce biofuels by overcoming the current challenges. However, further research effort is required to scale-up and improve the efficiency of these methods in the upstream and downstream technologies to make the cost of biofuels competitive with petroleum fuels.

  8. Fabrication of a multiplexed microfluidic system for scaled up production of cross-linked biocatalytic microspheres

    CSIR Research Space (South Africa)

    Mbanjwa, M

    2014-06-01

    Full Text Available the design and fabrication of a multiplexed microfluidic system for producing biocatalytic microspheres. The microfluidic system consists of an array of 10 parallel microfluidic circuits, for simultaneous operation to demonstrate increased production...

  9. Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

    KAUST Repository

    Perozziello, Gerardo; Candeloro, Patrizio; De Grazia, Antonio; Esposito, Francesco; Allione, Marco; Coluccio, Maria Laura; Tallerico, Rossana; Valpapuram, Immanuel; Tirinato, Luca; Das, Gobind; Giugni, Andrea; Torre, Bruno; Veltri, Pierangelo; Kruhne, Ulrich; Della Valle, Giuseppe; Di Fabrizio, Enzo M.

    2015-01-01

    In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where

  10. Interconnection blocks: a method for providing reusable, rapid, multiple, aligned and planar microfluidic interconnections

    DEFF Research Database (Denmark)

    Sabourin, David; Snakenborg, Detlef; Dufva, Hans Martin

    2009-01-01

    In this paper a method is presented for creating 'interconnection blocks' that are re-usable and provide multiple, aligned and planar microfluidic interconnections. Interconnection blocks made from polydimethylsiloxane allow rapid testing of microfluidic chips and unobstructed microfluidic observ...

  11. Better greenhouse gas emissions accounting for biofuels : A key to biofuels sustainability

    NARCIS (Netherlands)

    Peeters, Marjan; Yue, Taotao

    2016-01-01

    Biofuels are promoted by governments as a replacement for fossil fuels in the transport sector. However, according to current scientific evidence, their use does not necessarily significantly reduce greenhouse gas emissions. This article examines issues related to the regulation of biofuels’

  12. Policies for second generation biofuels: current status and future challenges

    Energy Technology Data Exchange (ETDEWEB)

    Egger, Haakan; Greaker, Mads; Potter, Emily

    2011-07-01

    Current state-of-the-art knowledge concludes that green house gas (GHG) emissions must be controlled and reduced within the next 30-40 years. The transport sector contributes almost a fifth of the current global emissions, and its share is likely to increase in the future. The US and a number of European countries have therefore introduced various support schemes for research and development (RandD) of low emission fuels that can potentially replace the current fossil fuels. One such alternative is biofuels. The advantage of biofuels are that it is easy to introduce into the transport sector. On the other hand, recent research papers question whether the supply of feedstock is sufficient, and to what extent biofuels lead to GHG emission reductions. This report reviews the current status of second generation biofuels. Second generation biofuels are made from cellulose, which according to our survey of the literature, is in more abundant supply than the first generation biofuels feedstocks. Furthermore, it seems to have the potential to reduce GHG emissions from the transport sector without leading to devastating land use changes, which recent critique has held against first generation biofuels. Given that governments have decided to support RandD of low emission fuels, we ask the following questions: Should second generation biofuels receive RandD support to the same extent as other low emission fuels like hydrogen? How should support schemes for second generation biofuels be designed? Second generation biofuels can be divided according to the production process into thermo-chemical and bio-chemical. With respect to the thermo-chemical process the potential for cost reductions seems to be low. On the other hand, ethanol made from cellulose using the biochemical conversion process is far from a ripe technology. Expert reports point to several potential technological breakthroughs which may reduce costs substantially. Hence, cellulosic ethanol, should receive direct

  13. Microfluidics on liquid handling stations (μF-on-LHS): a new industry-compatible microfluidic platform

    Science.gov (United States)

    Kittelmann, Jörg; Radtke, Carsten P.; Waldbaur, Ansgar; Neumann, Christiane; Hubbuch, Jürgen; Rapp, Bastian E.

    2014-03-01

    Since the early days microfluidics as a scientific discipline has been an interdisciplinary research field with a wide scope of potential applications. Besides tailored assays for point-of-care (PoC) diagnostics, microfluidics has been an important tool for large-scale screening of reagents and building blocks in organic chemistry, pharmaceutics and medical engineering. Furthermore, numerous potential marketable products have been described over the years. However, especially in industrial applications, microfluidics is often considered only an alternative technology for fluid handling, a field which is industrially mostly dominated by large-scale numerically controlled fluid and liquid handling stations. Numerous noteworthy products have dominated this field in the last decade and have been inhibited the widespread application of microfluidics technology. However, automated liquid handling stations and microfluidics do not have to be considered as mutually exclusive approached. We have recently introduced a hybrid fluidic platform combining an industrially established liquid handling station and a generic microfluidic interfacing module that allows probing a microfluidic system (such as an essay or a synthesis array) using the instrumentation provided by the liquid handling station. We term this technology "Microfluidic on Liquid Handling Stations (μF-on-LHS)" - a classical "best of both worlds"- approach that allows combining the highly evolved, automated and industry-proven LHS systems with any type of microfluidic assay. In this paper we show, to the best of our knowledge, the first droplet microfluidics application on an industrial LHS using the μF-on-LHS concept.

  14. Value Added Products from Renewable Biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Blum, Paul [Univ. of Nebraska, Lincoln, NE (United States)

    2014-07-31

    Cellulosic ethanol is an emerging biofuel that will make strong contributions to American domestic energy needs. In the US midwest the standard method for pretreatment of biomass uses hot acid to deconstruct lignocellulose. While other methods work, they are not in common use. Therefore it is necessary to work within this context to achieve process improvements and reductions in biofuel cost. Technology underlying this process could supplement and even replace commodity enzymes with engineered microbes to convert biomass-derived lignocellulose feedstocks into biofuels and valueadded chemicals. The approach that was used here was based on consolidated bioprocessing. Thermoacidophilic microbes belonging to the Domain Archaea were evaluated and modfied to promote deconvolution and saccharification of lignocellulose. Biomass pretreatment (hot acid) was combined with fermentation using an extremely thermoacidophilic microbial platform. The identity and fate of released sugars was controlled using metabolic blocks combined with added biochemical traits where needed. LC/MS analysis supported through the newly established Nebraska Bioenergy Facility provided general support for bioenergy researchers at the University of Nebraska. The primary project strategy was to use microbes that naturally flourish in hot acid (thermoacidophiles) with conventional biomass pretreatment that uses hot acid. The specific objectives were: to screen thermoacidophilic taxa for the ability to deconvolute lignocellulose and depolymerize associated carbohydrates; evaluate and respond to formation of “inhibitors” that arose during incubation of lignocellulose under heated acidic conditions; identify and engineer “sugar flux channeling and catabolic blocks” that redirect metabolic pathways to maximize sugar concentrations; expand the hydrolytic capacity of extremely thermoacidophilic microbes through the addition of deconvolution traits; and establish the Nebraska Bioenergy Facility (NBF

  15. Protein engineering for biofuel production: Recent development

    Directory of Open Access Journals (Sweden)

    Nisha Singh

    2016-09-01

    Full Text Available The unstable and unsure handiness of crude oil sources moreover the rising price of fuels have shifted international efforts to utilize renewable resources for the assembly of greener energy and a replacement which might additionally meet the high energy demand of the globe. Biofuels represent a sustainable, renewable, and also the solely predictable energy supply to fossil fuels. During the green production of Biofuels, several in vivo processes place confidence in the conversion of biomass to sugars by engineered enzymes, and the subsequent conversion of sugars to chemicals via designed proteins in microbial production hosts. Enzymes are indispensable within the effort to provide fuels in an ecologically friendly manner. They have the potential to catalyze reactions with high specificity and potency while not using dangerous chemicals. Nature provides an in depth assortment of enzymes, however usually these should be altered to perform desired functions in needed conditions. Presently available enzymes like cellulose are subject to tight induction and regulation systems and additionally suffer inhibition from numerous end products. Therefore, more impregnable and economical catalyst preparations ought to be developed for the enzymatic method to be more economical. Approaches like protein engineering, reconstitution of protein mixtures and bio prospecting for superior enzymes are gaining importance. Advances in enzyme engineering allow the planning and/or directed evolution of enzymes specifically tailored for such industrial applications. Recent years have seen the production of improved enzymes to help with the conversion of biomass into fuels. The assembly of the many of those fuels is feasible due to advances in protein engineering. This review discusses the distinctive challenges that protein engineering faces in the method of changing lignocellulose to biofuels and the way they're addressed by recent advances in this field.

  16. State-of-art in liquid biofuels

    Energy Technology Data Exchange (ETDEWEB)

    Poitrat, E. [Agency for Environment and Energy Management, Dept. of Agriculture and Bioenergies, Paris (France)

    2001-09-01

    European production of fuel alcohol/ETBE and VOME has now become significant and the research work undertaken i) to reduce the energy requirements and financial costs and ii) to improve environmental benefits, are of major importance. While optimisation still seems necessary, the gain to be achieved should come from the valorisation of co-products and increasing the size of production plants. Larger production plants will make it possible to implement techniques with higher investment costs but having greater energy efficiency. For VOME, the importance of the cost of producing the agricultural feedstock remains very critical, more so than the size of the industrial plant where the size of new plants (100 000 tons up to 250 000 tons) seems a good compromise. Wider use of biofuels will also depend on agricultural policy (setaside status), tax system and harmonisation of the European legislation on oxyfuels. It will improve the greenhouse effect in harmony with the KYOTO agreement. Environmental impacts, and more particularly their quantification in terms of externalities, remain a very crucial topic. In this situation, the quantity of produced biofuels is or will be more 2 millions tons now, including forecasted quantities, which represent 11% of the objective by 2010. Otherwise, the repartition of this current production is very unequal between the different countries of EC. The first production is planned by Germany with 1 millions tons, the second by France with 570 000 tons and the third by Spain with 306 000 tons. Smaller quantities are linked at Italy, Austria, Sweden and Belgium. Other countries have not biofuels production currently. (au)

  17. The European Union stakes on biofuels

    International Nuclear Information System (INIS)

    Laffon, M.

    2008-01-01

    The European Union has just published the environmental side of its action in the fight against climatic change. Like the third 'energy batch', these new legislative proposals are the continuation of the January 2007 communication of the commission which aimed at fixing the trends of the energy policy of the European Communities. Some measures of these last legislative proposals, in particular those treating of biofuels, are sometimes considered as too ambitious and even unrealistic. The overall proposals are waiting for the final adoption during the French presidency. (J.S.)

  18. Cyanobacteria as a platform for biofuel production

    Directory of Open Access Journals (Sweden)

    Nicole E Nozzi

    2013-09-01

    Full Text Available Cyanobacteria have great potential as a platform for biofuel production because of their fast growth, ability to fix carbon dioxide gas, and their genetic tractability. Furthermore they do not require fermentable sugars or arable land for growth and so competition with cropland would be greatly reduced. In this perspective we discuss the challenges and areas for improvement most pertinent for advancing cyanobacterial fuel production, including: improving genetic parts, carbon fixation, metabolic flux, nutrient requirements on a large scale, and photosynthetic efficiency using natural light.

  19. Ensuring sustainability in developing world biofuel productoin

    CSIR Research Space (South Africa)

    Von Maltitz, Graham P

    2009-06-01

    Full Text Available el N at io n al an d in te rn at io n al liq u id fu el s bl en ds Type 1 projects E.g. Mali Folkecentre Ghana Dumpong Biofuels See text box A and B Type 4 projects E.g. Large scale commercial plantations... approach Mali farmer growing jatropha as a fuel source to fuel 3 X 100 KW generators that will provide power to his village Brazilian ethanol production from large scale mechanised sugar cane fields Is certification and setting...

  20. Coproduction of bioethanol with other biofuels

    DEFF Research Database (Denmark)

    Ahring, Birgitte Kiær; Westermann, Peter

    2007-01-01

    Large scale transformation of biomass to more versatile energy carriers has most commonly been focused on one product such as ethanol or methane. Due to the nature of the biomass and thermodynamic and biological constraints, this approach is not optimal if the energy content of the biomass...... pilot-scale biorefineries for multiple fuel production and also discuss perspectives for further enhancement of biofuel yields from biomass. The major fuels produced in this refinery are ethanol, hydrogen, and methane. We also discuss the applicability of our biorefinery concept as a bolt-on plant...