WorldWideScience

Sample records for environmental uv radiation

  1. Effects of environmental and artificial UV-B radiation on freshwater prawn Macrobrachium olfersi embryos

    Energy Technology Data Exchange (ETDEWEB)

    Nazari, Evelise Maria [Programa de Pos-Graduacao em Ciencias Morfologicas, Instituto de Ciencias Biomedicas, Universidade Federal do Rio de Janeiro, 21949-902 Rio de Janeiro, RJ (Brazil); Universidade Federal de Santa Catarina, Departamento de Biologia Celular, Embriologia e Genetica, Campus Universitario, 88040-900 Florianopolis, SC (Brazil); Ammar, Dib [Universidade do Oeste de Santa Catarina, Departamento de Biologia, Campus Universitario, 89600-000 Joacaba, SC (Brazil); Bem, Andreza Fabro de; Latini, Alexandra [Universidade Federal de Santa Catarina, Departamento de Bioquimica, Campus Universitario, 88040-900 Florianopolis, SC (Brazil); Mueller, Yara Maria Rauh [Universidade Federal de Santa Catarina, Departamento de Biologia Celular, Embriologia e Genetica, Campus Universitario, 88040-900 Florianopolis, SC (Brazil); Allodi, Silvana, E-mail: sallodi@histo.ufrj.br [Programa de Pos-Graduacao em Ciencias Morfologicas, Instituto de Ciencias Biomedicas, Universidade Federal do Rio de Janeiro, 21949-902 Rio de Janeiro, RJ (Brazil)

    2010-06-01

    The recent decrease of the stratospheric ozone has resulted in an increase of ultraviolet-B (UV-B) radiation reaching the Earth's surface. In freshwater ecosystems with transparent water, UV-B rays easily penetrate and potentially cause harmful effects to organisms. In this study, embryos of the prawn Macrobrachium olfersi were used to evaluate the impact of UV-B rays in freshwater environments. We observed three groups of embryos: the first was to assess whether UV-B radiation produced morphological defects and/or biochemical impairments in the laboratory. The second was to check whether embryos with the same impairments as those observed in the laboratory were found in their environment, under natural solar radiation. The third group was the non-irradiated control. The embryos irradiated with 310 mW cm{sup -2} UV-B for 30 min showed morphological alterations similar to those observed in embryos from the environmental control group. The most important effects of the UV-B radiation observed in M. olfersi embryos were morphological (1.2% of the total number of embryos from the environment and 2.8% of the total number of irradiated embryos), pigmentation changes in the eyes (78.0% of the total number of embryos from the environment and 98.9% of the total number of irradiated embryos), and disruption of the chromatophores (46.9% of the total number of embryos from the environment and 95.5% of the total number of irradiated embryos). We also observed an increase in egg volume, which was accompanied by a significant increase in water content in UV-B irradiated groups when compared with aquaria control embryos. In addition, a significant decrease in the mitotic index in eggs exposed to UV-B radiation was detected (0.17 for the embryos from the aquaria control, 0.10 for the embryos of the environmental control, and 0.04 for the irradiated groups). The low levels of NPSH and high levels of TBARS indicated that UV-B rays directly compromised the antioxidant function of

  2. Effects of environmental and artificial UV-B radiation on freshwater prawn Macrobrachium olfersi embryos

    International Nuclear Information System (INIS)

    Nazari, Evelise Maria; Ammar, Dib; Bem, Andreza Fabro de; Latini, Alexandra; Mueller, Yara Maria Rauh; Allodi, Silvana

    2010-01-01

    The recent decrease of the stratospheric ozone has resulted in an increase of ultraviolet-B (UV-B) radiation reaching the Earth's surface. In freshwater ecosystems with transparent water, UV-B rays easily penetrate and potentially cause harmful effects to organisms. In this study, embryos of the prawn Macrobrachium olfersi were used to evaluate the impact of UV-B rays in freshwater environments. We observed three groups of embryos: the first was to assess whether UV-B radiation produced morphological defects and/or biochemical impairments in the laboratory. The second was to check whether embryos with the same impairments as those observed in the laboratory were found in their environment, under natural solar radiation. The third group was the non-irradiated control. The embryos irradiated with 310 mW cm -2 UV-B for 30 min showed morphological alterations similar to those observed in embryos from the environmental control group. The most important effects of the UV-B radiation observed in M. olfersi embryos were morphological (1.2% of the total number of embryos from the environment and 2.8% of the total number of irradiated embryos), pigmentation changes in the eyes (78.0% of the total number of embryos from the environment and 98.9% of the total number of irradiated embryos), and disruption of the chromatophores (46.9% of the total number of embryos from the environment and 95.5% of the total number of irradiated embryos). We also observed an increase in egg volume, which was accompanied by a significant increase in water content in UV-B irradiated groups when compared with aquaria control embryos. In addition, a significant decrease in the mitotic index in eggs exposed to UV-B radiation was detected (0.17 for the embryos from the aquaria control, 0.10 for the embryos of the environmental control, and 0.04 for the irradiated groups). The low levels of NPSH and high levels of TBARS indicated that UV-B rays directly compromised the antioxidant function of the

  3. Effects of UV radiation on phytoplankton

    Science.gov (United States)

    Smith, Raymond C.; Cullen, John J.

    1995-07-01

    It is now widely documented that reduced ozone will result in increased levels of ultraviolet (UV) radiation, especially UV-B (280-320nm), incident at the surface of the earth [Watson, 1988; Anderson et al., 1991; Schoeberl and Hartmann, 1991; Frederick and Alberts, 1991; WMO, 1991; Madronich, 1993; Kerr and McElroy, 1993], and there is considerable and increasing evidence that these higher levels of UV-B radiation may be detrimental to various forms of marine life in the upper layers of the ocean. With respect to aquatic ecosystems, we also know that this biologically- damaging mid-ultraviolet radiation can penetrate to ecologically- significant depths in marine and freshwater systems [Jerlov, 1950; Lenoble, 1956; Smith and Baker, 1979; Smith and Baker, 1980; Smith and Baker, 1981; Kirk et al., 1994]. This knowledge, plus the dramatic decline in stratospheric ozone over the Antarctic continent each spring, now known to be caused by anthropogenically released chemicals [Solomon, 1990; Booth et al., 1994], has resulted in increased UV-environmental research and a number of summary reports. The United Nations Environmental Program (UNEP) has provided recent updates with respect to the effects of ozone depletion on aquatic ecosystems (Hader, Worrest, Kumar in UNEP 1989, 1991, Hader, Worrest, Kumar and Smith UNEP 1994) and the Scientific Committee on Problems of the Environment (SCOPE) has provided [SCOPE, 1992] a summary of the effects of increased UV radiation on biological systems. SCOPE has also reported [SCOPE, 1993] on the effects of increased UV on the biosphere. In addition, several books have recently been published reviewing various aspects of environmental UV photobiology [Young et al., 1993], UV effects on humans, animals and plants [Tevini, 1993], the biological effects of UV radiation in Antarctica [Weiler and Penhale, 1994], and UV research in freshwater ecosystems [Williamson and Zagarese, 1994]. Several other reviews are relevant [NAS, 1984; Caldwell

  4. MEDICAL AND ENVIRONMENTAL EFFECTS OF UV RADIATION.

    Energy Technology Data Exchange (ETDEWEB)

    SUTHERLAND, B.M.

    2001-07-26

    Organisms living on the earth are exposed to solar radiation, including its ultraviolet (UV) components (for general reviews, the reader is referred to Smith [1] and Young et al. [2]). UV wavelength regions present in sunlight are frequently designated as UVB (290-320 nm) and UVA (320-400 nm). In today's solar spectrum, UVA is the principal UV component, with UVB present at much lower levels. Ozone depletion will increase the levels of UVB reaching the biosphere, but the levels of UVA will not be changed significantly [3]. Because of the high efficiency of UVB in producing damage in biological organisms in the laboratory experiments, it has sometimes been assumed that UVA has little or no adverse biological effects. However, accumulating data [4, 5], including action spectra (efficiency of biological damage as a function of wavelength of radiation; see Section 5) for DNA damage in alfalfa seedlings [6], in human skin [7], and for a variety of plant damages (Caldwell, this volume) indicate that UVA can induce damage in DNA in higher organisms. Thus, understanding the differential effects of UVA and UVB wavebands is essential for estimating the biological consequences of stratospheric ozone depletion.

  5. UV Radiation and the Skin

    Directory of Open Access Journals (Sweden)

    Timothy Scott

    2013-06-01

    Full Text Available UV radiation (UV is classified as a “complete carcinogen” because it is both a mutagen and a non-specific damaging agent and has properties of both a tumor initiator and a tumor promoter. In environmental abundance, UV is the most important modifiable risk factor for skin cancer and many other environmentally-influenced skin disorders. However, UV also benefits human health by mediating natural synthesis of vitamin D and endorphins in the skin, therefore UV has complex and mixed effects on human health. Nonetheless, excessive exposure to UV carries profound health risks, including atrophy, pigmentary changes, wrinkling and malignancy. UV is epidemiologically and molecularly linked to the three most common types of skin cancer, basal cell carcinoma, squamous cell carcinoma and malignant melanoma, which together affect more than a million Americans annually. Genetic factors also influence risk of UV-mediated skin disease. Polymorphisms of the melanocortin 1 receptor (MC1R gene, in particular, correlate with fairness of skin, UV sensitivity, and enhanced cancer risk. We are interested in developing UV-protective approaches based on a detailed understanding of molecular events that occur after UV exposure, focusing particularly on epidermal melanization and the role of the MC1R in genome maintenance.

  6. Environmental effects of ozone depletion, UV radiation and interactions with climate change: UNEP Environmental Effects Assessment Panel, update 2017

    Science.gov (United States)

    The Environmental Effects Assessment Panel (EEAP) is one of three Panels of experts that inform the Parties to the Montreal Protocol. The EEAP focuses on the effects of UV radiation on human health, terrestrial and aquatic ecosystems, air quality, and materials, as well as on the...

  7. The effectiveness of UV-C radiation for facility-wide environmental disinfection to reduce health care-acquired infections.

    Science.gov (United States)

    Napolitano, Nathanael A; Mahapatra, Tanmay; Tang, Weiming

    2015-12-01

    Health care-acquired infections (HAIs) constitute an increasing threat for patients worldwide. Potential contributors of HAIs include environmental surfaces in health care settings, where ultraviolet-C radiation (UV-C) is commonly used for disinfection. This UV-C intervention-based pilot study was conducted in a hospital setting to identify any change in the incidence of HAIs before and after UV-C intervention, and to determine the effectiveness of UV-C in reducing pathogens. In a hospital in Culver City, CA, during 2012-2013, bactericidal doses of UV-C radiation (254 nm) were delivered through a UV-C-based mobile environmental decontamination unit. The UV-C dosing technology and expertise of the specifically trained personnel were provided together as a dedicated service model by a contracted company. The incidence of HAIs before and after the intervention period were determined and compared. The dedicated service model dramatically reduced HAIs (incidence difference, 1.3/1000 patient-days, a 34.2% reduction). Reductions in the total number and incidence proportions (28.8%) of HAIs were observed after increasing and maintaining the coverage of UV-C treatments. The dedicated service model was found to be effective in decreasing the incidence of HAIs, which could reduce disease morbidity and mortality in hospitalized patients. This model provides a continuously monitored and frequently UV-C-treated patient environment. This approach to UV-C disinfection was associated with a decreased incidence of HAIs. Copyright © 2015 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.

  8. How do environmental and behavioral factors impact ultraviolet radiation effects on health: the RISC-UV Project

    Science.gov (United States)

    Correa, M. P.; Godin-Beekmann, S.; Haeffelin, M.; Saiag, P.; Mahe, E.; Brogniez, C.; Dupont, J. C.; Pazmiño, A.; Auriol, F.; Bonnel, B.

    2009-04-01

    Introduction: RISC-UV is a research project on "Impact of climate change on ultraviolet radiation and risks for health", a research project in which physicists, meteorologists and physicians work together to assess the relative role played by environmental and behavioral factors in the UV-related diseases as skin cancer and vitamin D deficiency. Environmental factors are related to the role played by the alteration in intensity of UV radiation at the Earth's surface resulting from variation in several factors affected by climate change and human activities: stratospheric ozone, cloud cover, aerosols and the reflectivity of the surface. On the other hand, behavioral factors are related to the sun over/underexposure and the correct use of sun-protection (hats, caps, sunglasses, sunscreen lotion, etc.). RISC-UV is organized around three main areas: 1) Organization of a workshop, scheduled for January 2009, which aims to describe the state of the art in the subject within each community and define the requirements of pathologists for epidemiological studies; 2) A pilot study intended to evaluate the consistency between UV measurements delivered simultaneously by satellite-based instruments, ground instruments, radiometers and individual dosimeters. This study is based on measurements campaigns and an analysis of the long-term consistency of data series relating to UV radiation and associated parameters; and 3) Analysis of the weights of medical, behavioral and environmental parameters involved in skin carcinogenesis. A detailed description of these areas can be found in http://www.gisclimat.fr/Doc/GB/D_projects/RISC-UV_GB.html. This presentation focuses on the first results of the UV experimental measurements performed between September 8th and October 8th 2008 in Palaiseau, France (48.7˚ N; 2.2˚ E; 170m - Haeffelin et al., 2005). A second campaign is foreseen for the spring of 2009. The purpose of these campaigns is to obtain, analyze and quantitatively link the

  9. Effects and mechanism of UV-B radiation on rice growth

    International Nuclear Information System (INIS)

    Gao Xiaoxiao; Gao Zhaohua; Zu Yanqun

    2009-01-01

    The enhancement of UV-B radiation influences the growth of rice and physiology in different levels and this performances as changes in morphology destroyed photosynthetic system unstable anti-oxidation system changes of endogenous hormone content exacerbated rice diseases decreased biomass and developmental stage delay. Through the establishment of the response index we can evaluate the varietal differences in responses of the rice to UV-B radiation. Reasons for such varietal differences were differences in rice gene physiology and morphology developmental stage and environmental factors. The main mechanism in responses of the rice to UV-B radiation was induction of flavonoid compounds and accumulation of anthocyanins. Based on the analysis of the influence of enhanced UV-B radiation to rice and the varietal differences in responses to UV-B radiation and mechanism of rice the direction of the further research about the relationship between the rice and UV-B was put forward

  10. Role of ultraviolet (UV) disinfection in infection control and environmental cleaning.

    Science.gov (United States)

    Qureshi, Zubair; Yassin, Mohamed H

    2013-06-01

    Ultraviolet (UV) radiation is capable of disinfecting surfaces, water and air. The UV technology was used for many years. However, safer and more effective delivery systems of UV radiation, make it a very useful option for disinfection. Effective disinfection of environmental surfaces is a key step in the prevention of spread of infectious agents. The traditional manual cleaning is essential in assuring adequate elimination of contamination. However, terminal cleaning is frequently suboptimal or unpredictable in many circumstances. UV-C radiation is an adjunctive disinfectant new technology that could kill a wide array of microorganisms including both vegetative and spore forming pathogens. The technology is getting more affordable and has produced consistent reproducible significant reduction of bacterial contamination.

  11. Epidermal UV-A absorbance and whole-leaf flavonoid composition in pea respond more to solar blue light than to solar UV radiation.

    Science.gov (United States)

    Siipola, Sari M; Kotilainen, Titta; Sipari, Nina; Morales, Luis O; Lindfors, Anders V; Robson, T Matthew; Aphalo, Pedro J

    2015-05-01

    Plants synthesize phenolic compounds in response to certain environmental signals or stresses. One large group of phenolics, flavonoids, is considered particularly responsive to ultraviolet (UV) radiation. However, here we demonstrate that solar blue light stimulates flavonoid biosynthesis in the absence of UV-A and UV-B radiation. We grew pea plants (Pisum sativum cv. Meteor) outdoors, in Finland during the summer, under five types of filters differing in their spectral transmittance. These filters were used to (1) attenuate UV-B; (2) attenuate UV-B and UV-A radiation signals that extend into the visible region of the solar spectrum. Furthermore, solar blue light instead of solar UV-B radiation can be the main regulator of phenolic compound accumulation in plants that germinate and develop outdoors. © 2014 John Wiley & Sons Ltd.

  12. Effects of ozone depletion and UV-B radiation on humans and the environment

    Energy Technology Data Exchange (ETDEWEB)

    Solomon, K.R. [Guelph Univ., ON (Canada). Centre for Toxicology

    2008-03-15

    This paper summarized current research related to the effects of ultraviolet (UV-B) radiation on human health and the environment. Effects included direct responses in human as well as effects on biogeochemistry and the environmental cycling of substances. UV radiation has many harmful effects on the skin, eyes, and immune systems of humans. Skin cancer is a leading cause of death among fair-skinned populations exposed to UV radiation. The role of UV radiation in cataract formation was discussed, as well as issues related to the suppression of immune responses. The link between sunlight exposure and vitamin D levels in human populations was examined. The effects of UV radiation on terrestrial and aquatic ecosystems were reviewed. Issues related to biogeochemistry and atmospheric processes were discussed. The review suggested that changes in the intensity of solar UV radiation due to ozone depletion will have important repercussions for all organisms on the planet. It was concluded that the combined effects of UV-B radiation and climate change will not be easy to predict. 201 refs., 4 figs.

  13. UV radiation and its effects. Proceedings

    International Nuclear Information System (INIS)

    1993-01-01

    The National Science Strategy Committee for Climate Change was established in 1991 by the New Zealand Minister of Research, Science and Technology. It advises government through the Minister on research priorities and on levels of expenditure appropriate in various topics relating to climate change. An additional role is to promote coordination between research groups and the user communities to ensure an appropriate range of research strategies. To assist with implementing the latter aspects the NSS Committee will organise workshops on specific aspects of atmosphere and climate change, with a broad spectrum of participants. The first of these was the Workshop on UV Radiation and its Effects held in Christchurch on 20-21 May 1993. The workshop had 40 participants, including representatives from specialist science groups, medicine, veterinary science, farming, forestry and environmental groups. This publication will update the interested reader, whether scientist or lay-person, on the current state of knowledge on changing UV radiation levels and potential problems. As the summaries of papers show, research on ozone levels and on UV radiation and its effects is particularly appropriate for New Zealand scientists with their access to sites covering a wide range of latitudes from Antarctica to the Pacific Islands. New Zealand is part of an important international monitoring network, measuring local stratospheric ozone levels and related surface UV radiation levels. There are concerns about increasing UVB levels and the consequent effects on human health, plant and tree growth, and phytoplankton growth in the oceans. Priorities for further work on these areas are included in the summary of the workshop. (author). 13 figs.; 5 tabs

  14. Impact of UV Radiation on Genome Stability and Human Health.

    Science.gov (United States)

    Roy, Sujit

    2017-01-01

    Gradual depletion of the atmospheric ozone layer during the past few years has increased the incidence of solar UV radiation specifically the UV-C on earth's surface is one of the major environmental concerns because of the harmful effects of this radiation in all forms of life. The solar UV radiation including the harmful wavelength range of UV-B (280-320 nm) represents a significant climatic stress for both animals and plants, causing damage to the fundamental biomolecules such as DNA, proteins and lipids, thus activating genotoxic stress and induces genome instability. When DNA absorbs UV-B light, energy from the photon causes covalent linkages to form between adjacent pyrimidine bases, creating photoproducts, primarily cyclobutane pyrimidine dimers (CPDs) and pyrimidine-6,4-pyrimidinone photoproduct (6,4PPs). Pyrimidine dimers create distortions in the DNA strands and therefore can inhibit DNA replication as well transcription. Lack of efficient repair of UV-induced DNA damage may induce the formation of DNA double stand breaks (DSBs), one of the serious forms of damage in DNA double helix, as well as oxidative damage. Unrepaired DSBs in the actively dividing somatic cells severely affect cell growth and development, finally results in loss of cell viability and development of various diseases, such as cancer in man.This chapter mainly highlights the incidence of solar UV-radiation on earth's surface along with the formation of major types of UV-induced DNA damage and the associated repair mechanisms as well as methods of detecting DNA damage and finally our present understanding on the impact on solar UV radiation on human health.

  15. Effects of UV radiation on freshwater metazooplankton

    International Nuclear Information System (INIS)

    Tartarotti, B.

    1999-06-01

    There is evidence that fluxes of solar ultraviolet-B radiation (UV-B, 290-320 nm) are increasing over wide parts of the earth's surface due to stratospheric ozone depletion. UV radiation (290-400 nm) can have damaging effects on biomolecules and cell components that are common to most living organisms. The aim of this thesis is to gain a more thorough understanding of the potential impacts of solar radiation on freshwater metazooplankton. To detect UV-vulnerability in zooplankton populations dominating the zooplankton community of two clear-water, high mountain lakes located one in the Austrian Alps and another in the Chilean Andes, the survival of two copepod species was studied. The organisms were exposed to a 10- to 100-fold increase in UV-B radiation compared to those levels found at their natural, maximum daytime distribution. Both species vertically migrate and are pigmented. UV-absorbing compounds with a maximum absorption at ∼334 nm were also detected. Cyclops abyssorum tatricus, a common cyclopoid copepod species of Alpine lakes, was highly resistant to UV-B radiation and no significant lethal effect was observed. The calanoid copepod Boeckella gracilipes, frequent in Andean lakes, had a mortality ∼5 times higher in the treatment receiving full sunlight than in the UV-B excluded treatment (3.2 %) only when exposed for 70 h. The resistance of B. gracilipes was higher than that reported in the literature for the same species suggesting the existence of intraspecific differences in UV sensitivity. Survival, fecundity and development of the zooplankton community of a clear-water, high elevation Andean lake (33 o S) were studied with mesocosms experiments after prolonged UV exposure (48 days). When exposed to full sunlight, the population of the cladoceran Chydorus sphaericus and the rotifer Lepadella ovalis were strongly inhibited by UV-B, whereas both species were resistant to UV-A radiation. Conversely, UV-B radiation had no effect on the survival of the

  16. The global and UV-B radiation over Egypt

    OpenAIRE

    BASSET, H. A.; KORANY, M. H.

    2007-01-01

    This work studies the relation between UV-B radiation and global radiation over Egypt. The relationships between the global solar radiation and UV-B radiation at four stations in Egypt have been studied, and linear empirical formulas for estimating UV-B from global radiation at these stations has been deduced. The deduced equations were applied to calculate the UV-B radiation for other stations where measurements were unavailable, using records of global radiation at these stations. Because o...

  17. Protection policies for ionizing and UV radiation

    International Nuclear Information System (INIS)

    Bosnjakovic, B.F.M.

    1987-01-01

    Although ultraviolet radiation is generally considered as being part of non-ionizing radiation, the existing similarities with ionizing radiation are too striking to be overseen. A comparison of these two agents is becoming important in view of the increasing awareness of various environmental and health risks and the tendency to develop more uniform risk management policies with respect to the different physical and chemical agents. This paper explores the similarities and differences of UV and ionizing radiation from the point of view of policies either adopted or in development. Policy determinants include, among others, the following factors: biological effects, dosimetric quantities, relative contribution to exposure from different sources, hazard potential of different sources, quantification of detrimental consequences, public perception of the radiation hazards and regulation developments. These factors are discussed

  18. Highly Sensitive Detection of UV Radiation Using a Uranium Coordination Polymer.

    Science.gov (United States)

    Liu, Wei; Dai, Xing; Xie, Jian; Silver, Mark A; Zhang, Duo; Wang, Yanlong; Cai, Yawen; Diwu, Juan; Wang, Jian; Zhou, Ruhong; Chai, Zhifang; Wang, Shuao

    2018-02-07

    The accurate detection of UV radiation is required in a wide range of chemical industries and environmental or biological related applications. Conventional methods taking advantage of semiconductor photodetectors suffer from several drawbacks such as sophisticated synthesis and manufacturing procedure, not being able to measure the accumulated UV dosage as well as high defect density in the material. Searching for new strategies or materials serving as precise UV dosage sensor with extremely low detection limit is still highly desirable. In this work, a radiation resistant uranium coordination polymer [UO 2 (L)(DMF)] (L = 5-nitroisophthalic acid, DMF = N,N-dimethylformamide, denoted as compound 1) was successfully synthesized through mild solvothermal method and investigated as a unique UV probe with the detection limit of 2.4 × 10 -7 J. On the basis of the UV dosage dependent luminescence spectra, EPR analysis, single crystal structure investigation, and the DFT calculation, the UV-induced radical quenching mechanism was confirmed. Importantly, the generated radicals are of significant stability which offers the opportunity for measuring the accumulated UV radiation dosage. Furthermore, the powder material of compound 1 was further upgraded into membrane material without loss in luminescence intensity to investigate the real application potentials. To the best of our knowledge, compound 1 represents the most sensitive coordination polymer based UV dosage probe reported to date.

  19. UV-B Radiation Contributes to Amphibian Population Declines

    Science.gov (United States)

    Blaustein, Andrew

    2007-05-01

    UV-B (280-315 nm) radiation is the most significant biologically damaging radiation at the terrestrial surface. At the organismal level, UV-B radiation can slow growth rates, cause immune dysfunction and result in sublethal damage. UV-B radiation can lead to mutations and cell death. Over evolutionary time, UV radiation has been an important stressor on living organisms. Natural events, including impacts from comets and asteroids, volcanic activity, supernova explosions and solar flares, can cause large-scale ozone depletion with accompanying increases in UV radiation. However, these natural events are transient. Moreover, the amount of ozone damage due to natural events depends upon a number of variables, including the magnitude of the event. This is different from modern-day human-induced production of chlorofluorocarbons (CFCs) and other chemicals that deplete stratospheric ozone continuously, resulting in long-term increases in UV-B radiation at the surface of the earth. We will briefly review the effects of UV-B exposure in one group of aquatic organisms_amphibians. UV-B has been implicated as a possible factor contributing to global declines and range reductions in amphibian populations.

  20. Photosynthesis and pigment production in Liquidambar styraciflua - developmental and UV-B radiation effects

    International Nuclear Information System (INIS)

    Dillenburg, L.R.; Sullivan, J.H.; Teramura, A.H.

    1993-01-01

    Leaf expansion is very sensitive to different environmental stresses. This study describes ontogenetic changes in leaf size and physiology of Liquidambar styraciflua seedlings grown under UV-B irradiance levels simulating 0% (control), 16% (low) and 25% (high) stratospheric ozone reductions. Leaf size, light- and CO 2 -saturated rates of O 2 evolution (A max ), and concentration of chlorophylls (chl), and UV-B absorbing pigments were measured over a 4-week period. Specific leaf weight, A max and chl concentration increased with leaf age, except for a peak in A max at early development. Chlorophyll b concentration increased at a slower rate than chl a. Recently unfurled leaves has the greatest concentration of UV-B absorbing pigments. The effect of UV-B radiation on leaf growth and physiology were small and not dose-dependent. Expansion of leaves exposed to low UV-B was slightly delayed compared to controls (1.663 vs. 1.90 cm 2 /day), but final leaf size was unaffected by UV-B radiation. Physiological effects were less pronounced during the rapid expansion period. High UV-B tended to promote, while low UV-B inhibited accumulation of chl, especially chl a. In contrast, concentration of UV-B absorbing compounds was promoted only by low UV-B. The small inhibitory effects of UV-B on leaf growth and physiology suggests a high tolerance of the species to damaging UV-B radiation

  1. The effect of exposure to enhanced UV-B radiation on the penetration of monochromatic and polychromatic UV-B radiation in leaves of Brassica napus

    International Nuclear Information System (INIS)

    Cen, Y.-P.; Bornman, J.F.

    1993-01-01

    Using quartz optical fibres, penetration of both monochromatic (310 nm) and polychromatic UV-B (280–320 nm) radiation in leaves of Brassica napus L. (cv. Ceres) was measured. Plants were grown under either visible light (750 μmol m −2 s −1 photosynthetically active radiation) or with the addition of 8. 9 KJ m −2 day −1 biologically effective UV-B (UV-B BE ) radiation. Results showed that of the 310 nm radiation that penetreated the leaf, 90% was within the intial one third of the leaf with high attenuation in the leaf epidermis, especially in UV-treated plants. Polychromatic UV-B radiation, relative to incident radiation, showed a relatively uniform spectral distribution within the leaf, except for collimated radiation. Over 30% of the UV-screening pigments in the leaf, including flavonoids, were found in the adaxial epidermal layer, making this layer less transparent to UV-B radiation than the abaxial epidermis, which contained less than 12% of the UV-screening pigments. UV-screening pigments increased by 20% in UV-treated leaves relative to control leaves. Densely arranged epicuticular wax on the adaxial leaf surface of UV-treated plants may have further decreased penetration of UV-B radiation by reflectance. An increased leaf thickness, and decreases in leaf area and leaf dry weight were also found for UV-treated plants. (author)

  2. Study on DNA damages induced by UV radiation

    International Nuclear Information System (INIS)

    Doan Hong Van; Dinh Ba Tuan; Tran Tuan Anh; Nguyen Thuy Ngan; Ta Bich Thuan; Vo Thi Thuong Lan; Tran Minh Quynh; Nguyen Thi Thom

    2015-01-01

    DNA damages in Escherichia coli (E. coli) exposed to UV radiation have been investigated. After 30 min of exposure to UV radiation of 5 mJ/cm"2, the growth of E. coli in LB broth medium was about only 10% in compared with non-irradiated one. This results suggested that the UV radiation caused the damages for E. coli genome resulted in reduction in its growth and survival, and those lesions can be somewhat recovered. For both solutions of plasmid DNAs and E. coli cells containing plasmid DNA, this dose also caused the breakage on single and double strands of DNA, shifted the morphology of DNA plasmid from supercoiled to circular and linear forms. The formation of pyrimidine dimers upon UV radiation significantly reduced when the DNA was irradiated in the presence of Ganoderma lucidum extract. Thus, studies on UV-induced DNA damage at molecular level are very essential to determine the UV radiation doses corresponding to the DNA damages, especially for creation and selection of useful radiation-induced mutants, as well as elucidation the protective effects of the specific compounds against UV light. (author)

  3. Sun, UV Radiation and Your Eyes

    Science.gov (United States)

    ... Sunglasses Sun Smart UV Safety Infographic The Sun, UV Radiation and Your Eyes Leer en Español: El ... Aug. 28, 2014 Keep an Eye on Ultraviolet (UV) Safety Eye medical doctors (ophthalmologists) caution us that ...

  4. Quantification of biologically effective environmental UV irradiance

    Science.gov (United States)

    Horneck, G.

    To determine the impact of environmental UV radiation on human health and ecosystems demands monitoring systems that weight the spectral irradiance according to the biological responses under consideration. In general, there are three different approaches to quantify a biologically effective solar irradiance: (i) weighted spectroradiometry where the biologically weighted radiometric quantities are derived from spectral data by multiplication with an action spectrum of a relevant photobiological reaction, e.g. erythema, DNA damage, skin cancer, reduced productivity of terrestrial plants and aquatic foodweb; (ii) wavelength integrating chemical-based or physical dosimetric systems with spectral sensitivities similar to a biological response curve; and (iii) biological dosimeters that directly weight the incident UV components of sunlight in relation to the effectiveness of the different wavelengths and to interactions between them. Most biological dosimeters, such as bacteria, bacteriophages, or biomolecules, are based on the UV sensitivity of DNA. If precisely characterized, biological dosimeters are applicable as field and personal dosimeters.

  5. UV radiation dependent flavonoid accumulation of Cistus laurifolius L

    International Nuclear Information System (INIS)

    Vogt, T.; Gülz, P.-G.; Reznik, H.

    1991-01-01

    Epicuticular and intracellular flavonoids of Cistus laurifolius grown with and without UV radiation in a phytotron as well as under natural garden conditions in the field were studied. The amount of intracellular flavonoid glycosides of leaves receiving UV-A radiation was two fold higher than that measured in the absence o f UV-A radiation, whether grown in the phytotron or in the field. Exposure of previously protected leaves to UV-A radiation increased the intracellular flavonoid glycoside content to that of unprotected leaves. The qualitative composition of intracellular flavonoid glycosides showed a reduced amount of quercetin-3-galactoside to the myricetin monosides when the leaves were grown without UV-A radiation in the field and in the phytotron. Epicuticular flavonoid aglycones were not influenced by UV radiation significantly. (author)

  6. UV radiation: sources, effects and risks of human and environmental exposure

    International Nuclear Information System (INIS)

    Eggink, G.J.; Slaper, H.

    1992-01-01

    This paper summarizes the principal results of a review study on UV- -exposure and UV related risks in the Netherlands. Both the present state of affairs and future developments are discussed, the latter partly based on model calculations. The sun is the main UV source to which the whole population is exposed. Solar exposure is estimated to amount at least 90% of the annual UV burden for the Dutch population. For certain groups in the population man made sources are estimated to contribute considerably to the yearly UV dose. Ozone depletion as a result of human activities, growing use of tungsten halogen lamps and increasing application of UV-sources in industry and medicine all tend to increase UV exposure. UV exposure can lead to a wide variety of health effects, among which the induction of skin cancer, skin aging, cataract formation and suppression of immune responses. Risk estimates of these health effects are available for skin cancer and to a lesser extend for cataracts. The estimated UV related skin cancer incidence rate in the Netherlands is 10 -3 per year (15 000 cases), and the associated mortality rate amounts to 6-25·10 -6 per year (90-400 deaths). The ozone depletion presently observed over the past decade (5% in the Netherlands), is expected to lead to an increased annual mortality rate due to skin cancer of 1,3·10 -6 per year. Environmental exposure can influence plant physiology and lead to a decrease of biomass in aquatic as well as terrestrial ecosystems. This may result in adverse effects on the foodweb and biodiversity of ecosystems. Quantitative risk estimates for these effects are very uncertain or lacking. (author)

  7. Radiative models for the evaluation of the UV radiation at the ground

    International Nuclear Information System (INIS)

    Koepke, P.

    2009-01-01

    The variety of radiative models for solar UV radiation is discussed. For the evaluation of measured UV radiation at the ground the basic problem is the availability of actual values of the atmospheric parameters that influence the UV radiation. The largest uncertainties are due to clouds and aerosol, which are highly variable. In the case of tilted receivers, like the human skin for most orientations, and for conditions like a street canyon or tree shadow, besides the classical radiative transfer in the atmosphere additional modelling is necessary. (authors)

  8. Manipulating Sensory and Phytochemical Profiles of Greenhouse Tomatoes Using Environmentally Relevant Doses of Ultraviolet Radiation.

    Science.gov (United States)

    Dzakovich, Michael P; Ferruzzi, Mario G; Mitchell, Cary A

    2016-09-14

    Fruits harvested from off-season, greenhouse-grown tomato plants have a poor reputation compared to their in-season, garden-grown counterparts. Presently, there is a gap in knowledge with regard to the role of UV-B radiation (280-315 nm) in determining greenhouse tomato quality. Knowing that UV-B is a powerful elicitor of secondary metabolism and not transmitted through greenhouse glass and some greenhouse plastics, we tested the hypothesis that supplemental UV-B radiation in the greenhouse will impart quality attributes typically associated with garden-grown tomatoes. Environmentally relevant doses of supplemental UV-B radiation did not strongly affect antioxidant compounds of fruits, although the flavonol quercetin-3-O-rutinoside (rutin) significantly increased in response to UV-B. Physicochemical metrics of fruit quality attributes and consumer sensory panels were used to determine if any such differences altered consumer perception of tomato quality. Supplemental UV-A radiation (315-400 nm) pre-harvest treatments enhanced sensory perception of aroma, acidity, and overall approval, suggesting a compelling opportunity to environmentally enhance the flavor of greenhouse-grown tomatoes. The expression of the genes COP1 and HY5 were indicative of adaptation to UV radiation, which explains the lack of marked effects reported in these studies. To our knowledge, these studies represent the first reported use of environmentally relevant doses of UV radiation throughout the reproductive portion of the tomato plant life cycle to positively enhance the sensory and chemical properties of fruits.

  9. UV inactivation: Combined effects of UV radiation and xenobiotics in two strains of Saccharomyces

    International Nuclear Information System (INIS)

    Lochmann, E.R.; Lochmann, G.

    1997-01-01

    The effects of eight chemicals on the inactivation rate of ultraviolet radiation on the colony building capabilities of two strains of Saccharomyces cervisae - a wild type strain and a mutant deficient in excision repair - were studied. The insecticide methoxychlor, the herbicide 2,4-dichlorophenoxyacetic acid, the fungicide pentachlorophenol and its metabolite tetrachlorohydroquinone, as well as the chemicals acrylonitrile and 2,3-dichloro-1-propene have no significant impact on the effects of UV radiation in Saccharomyces cerevisae. Depending on the concentration, trichloroethylene increases the sensitivity to UV radiation. The herbicide paraquat provides efficient protection against UV radiation at concentrations where a toxic effect cannot be observed even without UV. The results were rather similar for both strains. (orig.) [de

  10. Researchers lack data on trends in UV radiation at Earth's surface

    International Nuclear Information System (INIS)

    Zurer, P.S.

    1993-01-01

    Current anxiety about depletion of stratospheric ozone stems from the expected resulting increase in biologically damaging ultraviolet (UV) radiation at Earth's surface. Atmospheric ozone absorbs sunlight with wavelengths shorter than 320 nm--the highest-energy UV-B wavelengths (280-320 nm) that can damage DNA in living systems. But surprisingly, despite firm evidence the ozone layer is being eroded by chlorine and bromine from man-made compounds, very little information exists on how UV light intensity is changing. Solid data from Antarctica reveal that UV radiation soars under the ozone hole, where fully half of the atmospheric ozone is destroyed each spring. But elsewhere on the globe, where ozone has been thinning at a rate of a few percent per decade, the corresponding trends in UV intensity are not at all clear. In the late 1970s and early 1980s the problem of ozone depletion seemed solved. The US had banned the use of chlorofluorocarbons (CFCs) in aerosols. Model calculations were predicting CFCs would cause only a small loss of ozone by the second half of the 21st century. Costly monitoring of UV radiation commanded little attention. Attitudes began to change with the 1985 discovery of the Antarctic ozone hole. The National Science Foundation (NSF) established UV monitoring stations in the Antarctic in 1988, adding an Alaskan station in 1990. Both the Department of Agriculture (USDA) and the Environmental Protection Agency (EPA) have programs in the works that will eventually place monitoring stations across the US, but it will be many years before researchers have access to the kind of extensive database necessary to reliably evaluation long-term trends in UV intensity

  11. Radiative flux calculations at UV and visible wavelengths

    International Nuclear Information System (INIS)

    Grossman, A.S.; Grant, K.E.; Wuebbles, D.J.

    1993-10-01

    A radiative transfer model to calculate the short wavelength fluxes at altitudes between 0 and 80 km has been developed at LLNL. The wavelength range extends from 175--735 nm. This spectral range covers the UV-B wavelength region, 250--350 nm, with sufficient resolution to allow comparison of UV-B measurements with theoretical predictions. Validation studies for the model have been made for both UV-B ground radiation calculations and tropospheric solar radiative forcing calculations for various ozone distributions. These studies indicate that the model produces results which agree well with respect to existing UV calculations from other published models

  12. Temporal variation of optimal UV exposure time over Korea: risks and benefits of surface UV radiation

    Science.gov (United States)

    Lee, Y. G.; Koo, J. H.

    2015-12-01

    Solar UV radiation in a wavelength range between 280 to 400 nm has both positive and negative influences on human body. Surface UV radiation is the main natural source of vitamin D, providing the promotion of bone and musculoskeletal health and reducing the risk of a number of cancers and other medical conditions. However, overexposure to surface UV radiation is significantly related with the majority of skin cancer, in addition other negative health effects such as sunburn, skin aging, and some forms of eye cataracts. Therefore, it is important to estimate the optimal UV exposure time, representing a balance between reducing negative health effects and maximizing sufficient vitamin D production. Previous studies calculated erythemal UV and vitamin-D UV from the measured and modelled spectral irradiances, respectively, by weighting CIE Erythema and Vitamin D3 generation functions (Kazantzidis et al., 2009; Fioletov et al., 2010). In particular, McKenzie et al. (2009) suggested the algorithm to estimate vitamin-D production UV from erythemal UV (or UV index) and determined the optimum conditions of UV exposure based on skin type Ⅱ according to the Fitzpatrick (1988). Recently, there are various demands for risks and benefits of surface UV radiation on public health over Korea, thus it is necessary to estimate optimal UV exposure time suitable to skin type of East Asians. This study examined the relationship between erythemally weighted UV (UVEry) and vitamin D weighted UV (UVVitD) over Korea during 2004-2012. The temporal variations of the ratio (UVVitD/UVEry) were also analyzed and the ratio as a function of UV index was applied in estimating the optimal UV exposure time. In summer with high surface UV radiation, short exposure time leaded to sufficient vitamin D and erythema and vice versa in winter. Thus, the balancing time in winter was enough to maximize UV benefits and minimize UV risks.

  13. The effect of ultraviolet (UV)-B radiation on primary producers

    International Nuclear Information System (INIS)

    Germ, M.

    2003-01-01

    Ozone layer in stratosphere is thinning and consequently UV-B radiation on the Earth surface is increasing. Although there is a small portion of UV-B radiation in the solar radiation, it has strong influence on organisms. Targets of UV-B radiation and protective mechanisms in primary producers are described. In the framework of the international project we studied the effect of UV-B radiation on blue-greens, algae, mosses, lichens and vascular plants on the National Institute of Biology

  14. UV radiation sources for artificial skin tanning and protection

    International Nuclear Information System (INIS)

    Zivkovic, D.; Hrnjak, M.

    1999-01-01

    UV radiation sources for artificial tanning are more utilized at the last time. UV radiation is not harmless, so there are not safety devices for tanning. If people do not want to avoid exposure to their radiation, than it is necessary to take the prevention measure: strictly dose of UV radiation according to skin type, use of appropriate protective eye-wears and respect for inhibit of some medicaments and some cosmetic products use. (author)

  15. Alfalfa seedlings grown outdoors are more resistant to UV-induced DNA damage than plants grown in a UV-free environmental chamber

    International Nuclear Information System (INIS)

    Takayanagi, Shinnosuke; Trunk, J.G.; Sutherland, J.C.; Sutherland, B.M.

    1994-01-01

    The relative UV sensitivities of alfalfa seedlings grown outdoors versus plants grown in a growth chamber under UV-filtered cool white fluorescent bulbs have been determined using three criteria: (1) level of endogenous DNA damage as sites for the UV endonuclease from Micrococcus luteus, (2) susceptibility to pyrimidine dimer induction by a UV challenge exposure and (3) ability to repair UV-induced damage. We find that outdoor-grown plants contain approximately equal frequencies of endogenous DNA damages, are less susceptible to dimer induction by a challenge exposure of broad-spectrum UV and photorepair dimers more rapidly than plants grown in an environmental chamber under cool white fluorescent lamps plus a filter removes most UV radiation. These data suggest that plants grown in a natural environment would be less sensitive to UVB-induced damage than would be predicted on the basis of studies on plants grown under minimum UV. (author)

  16. Prediction of UV spectra and UV-radiation damage in actual plasma etching processes using on-wafer monitoring technique

    International Nuclear Information System (INIS)

    Jinnai, Butsurin; Fukuda, Seiichi; Ohtake, Hiroto; Samukawa, Seiji

    2010-01-01

    UV radiation during plasma processing affects the surface of materials. Nevertheless, the interaction of UV photons with surface is not clearly understood because of the difficulty in monitoring photons during plasma processing. For this purpose, we have previously proposed an on-wafer monitoring technique for UV photons. For this study, using the combination of this on-wafer monitoring technique and a neural network, we established a relationship between the data obtained from the on-wafer monitoring technique and UV spectra. Also, we obtained absolute intensities of UV radiation by calibrating arbitrary units of UV intensity with a 126 nm excimer lamp. As a result, UV spectra and their absolute intensities could be predicted with the on-wafer monitoring. Furthermore, we developed a prediction system with the on-wafer monitoring technique to simulate UV-radiation damage in dielectric films during plasma etching. UV-induced damage in SiOC films was predicted in this study. Our prediction results of damage in SiOC films shows that UV spectra and their absolute intensities are the key cause of damage in SiOC films. In addition, UV-radiation damage in SiOC films strongly depends on the geometry of the etching structure. The on-wafer monitoring technique should be useful in understanding the interaction of UV radiation with surface and in optimizing plasma processing by controlling UV radiation.

  17. Covalent dimerization of ribulose bisphosphate carboxylase subunits by UV radiation

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, R.M.B. [Universidade Tecnica, Lisbon (Portugal). Inst. Superior de Agronomia]|[Universidade Nova de Lisboa, Oeiras (Portugal). Instituto de Tecnologia Quimica e Biologica; Franco, E.; Teixeira, A.R.N. [Universidade Tecnica, Lisbon (Portugal). Inst. Superior de Agronomia

    1996-08-15

    The effect of UV radiation (UV-A, UV-B and UV-C) on ribulose bisphosphate carboxylase from a variety of plant species was examined. The exposition of plant leaves or the pure enzyme to UV radiation produced a UV-dependent accumulation of a 65 kDa polypeptide (P65). Different approaches were utilized to elucidate the origin and structure of P65: electrophoretic and fluorographic analyses of {sup 35}S-labelled ribulose biphosphate carboxylase exposed to UV radiation and immunological experiments using antibodies specific for P65, for the large and small subunits of ribulose biphosphate carboxylase and for high-molecular-mass aggregates of the enzyme. These studies revealed that P65 is a dimer, formed by the covalent, non-disulphide linkage of one small subunit with one large subunit of ribulose biphosphate carboxylase. For short periods of time (<1 h), the amount of P65 formed increased with the duration of the exposure to the UV radiation and with the energy of the radiation applied. Prolonged exposure to UV radiation (1-6 h) resulted in the formation of high-molecular-mass aggregates of ribulose biphosphate carboxylase. Formation of P65 was shown to depend on the native state of the protein, was stimulated by inhibitors of enzyme activity, and was inhibited by activators of enzyme activity. A UV-independent accumulation of P65 was also achieved by the in vitro incubation of plant crude extracts. However, the UV-dependent and the UV-independent formation of P65 seemed to occur by distinct molecular mechanisms. The UV-dependent accumulation of P65 was immunologically detected in all species examined, including Lemna minor, Arum italicum, Brassica oleracea, Triticum aestivum, Zea mays, Pisum sativum and Phaseolus vulgaris, suggesting that it may constitute a universal response to UV radiation, common to all photosynthetic tissues. (Author).

  18. Covalent dimerization of ribulose bisphosphate carboxylase subunits by UV radiation

    International Nuclear Information System (INIS)

    Ferreira, R.M.B.; Universidade Nova de Lisboa, Oeiras; Franco, E.; Teixeira, A.R.N.

    1996-01-01

    The effect of UV radiation (UV-A, UV-B and UV-C) on ribulose bisphosphate carboxylase from a variety of plant species was examined. The exposition of plant leaves or the pure enzyme to UV radiation produced a UV-dependent accumulation of a 65 kDa polypeptide (P65). Different approaches were utilized to elucidate the origin and structure of P65: electrophoretic and fluorographic analyses of 35 S-labelled ribulose biphosphate carboxylase exposed to UV radiation and immunological experiments using antibodies specific for P65, for the large and small subunits of ribulose biphosphate carboxylase and for high-molecular-mass aggregates of the enzyme. These studies revealed that P65 is a dimer, formed by the covalent, non-disulphide linkage of one small subunit with one large subunit of ribulose biphosphate carboxylase. For short periods of time (<1 h), the amount of P65 formed increased with the duration of the exposure to the UV radiation and with the energy of the radiation applied. Prolonged exposure to UV radiation (1-6 h) resulted in the formation of high-molecular-mass aggregates of ribulose biphosphate carboxylase. Formation of P65 was shown to depend on the native state of the protein, was stimulated by inhibitors of enzyme activity, and was inhibited by activators of enzyme activity. A UV-independent accumulation of P65 was also achieved by the in vitro incubation of plant crude extracts. However, the UV-dependent and the UV-independent formation of P65 seemed to occur by distinct molecular mechanisms. The UV-dependent accumulation of P65 was immunologically detected in all species examined, including Lemna minor, Arum italicum, Brassica oleracea, Triticum aestivum, Zea mays, Pisum sativum and Phaseolus vulgaris, suggesting that it may constitute a universal response to UV radiation, common to all photosynthetic tissues. (Author)

  19. Rapid maize leaf and immature ear responses to UV-B radiation

    International Nuclear Information System (INIS)

    Casati, P.; Morrow, D.; Fernandes, J.; Walbot, V.

    2011-01-01

    Plants have evolved adaptations to environmental factors, including solar radiation. In addition to acting as a developmental and physiological signal, UV-B photons also cause cellular damage. Elevated UV-B radiation has pleiotropic effects on plant development, morphology, and physiology, but the regulation of systemic responses is not well-understood. To gain a better understanding of the initial events in UV-B acclimation, we have analyzed a 10min to 1h time course of transcriptome responses in irradiated and shielded leaves, and immature maize ears to unravel the systemic physiological and developmental responses in exposed and shielded organs. To identify metabolites as possible signaling molecules, we looked for compounds that increased within 5-90 min in both irradiated and shielded leaves, to explain the kinetics of profound transcript changes within 1h. We found that myoinositol is one such candidate metabolite, and it also has support from RNA profiling: after 1h UV-B, transcripts for myoinositol-1-phosphate synthase, are decreased in both irradiated and shielded leaves suggesting down-regulation of biogenesis. We also demonstrate that if 0.1mM myoinositol is applied to leaves of greenhouse maize, some metabolites that are changed by UV-B are also changed similarly by the chemical treatment. (author)

  20. Effect of UV-B Radiation and Desiccation Stress on Photoprotective Compounds Accumulation in Marine Leptolyngbya sp.

    Science.gov (United States)

    Joshi, Devika; Mohandass, C; Dhale, Mohan

    2018-01-01

    Increased awareness regarding the harmful effects of ultraviolet (UV)-B radiation has led to the search for new sources of natural UV-B protecting compounds. Mycosporine-like amino acids are one of such promising compounds found in several organisms. Cyanobacteria are ideal organisms for isolation of these compounds due to their compatibility and adaptability to thrive under harsh environmental conditions. In the following investigation, we report the production of shinorine in Leptolyngbya sp. isolated from the intertidal region. Based on the spectral characteristics and liquid chromatography-mass spectrometry analysis, the UV-absorbing compound was identified as shinorine. To the best of our knowledge, this is the first report on the occurrence of shinorine in Leptolyngbya sp. We also investigated the effect of artificial UV-B radiation and periodic desiccation on chlorophyll-a, total carotenoids, and mycosporine-like amino acids (MAAs) production. The UV-B radiation had a negative effect on growth and chlorophyll concentration, whereas it showed an inductive effect on the production of total carotenoids and MAAs. Desiccation along with UV-B radiation led to an increase in the concentration of photoprotective compounds. These results indicate that carotenoids and MAAs thus facilitate cyanobacteria to avoid and protect themselves from the deleterious effects of UV-B and desiccation.

  1. Antiradiation UV Vaccine: UV Radiation, Biological effects, lesions and medical management - immune-therapy and immune-protection.

    Science.gov (United States)

    Popov, Dmitri; Jones, Jeffrey; Maliev, Slava

    Key Words: Ultraviolet radiation,Standard Erythema Dose(SED), Minimal Erythema Dose(MED), Sun Burns, Solar Dermatitis, Sun Burned Disease, DNA Damage,Cell Damage, Antiradiation UV Vaccine, Immune-Prophylaxis of Sun Burned Diseases, Immune-Prophylaxis of Sun Burns, Immune-Therapy of Sun-Burned Disease and Sun Burns,Basal Cell Carcinoma (BCC), Squamous Cell Carcinoma (SCC), Toxic Epidermal Necrolysis(TEN). Introduction: High doses of UV generated by solar source and artificial sources create an exposure of mammals and other species which can lead to ultraviolet(UV)radiation- associated disease (including erythema, epilation, keratitis, etc.). UV radiation belongs to the non-ionizing part of the electromagnetic spectrum and ranges between 100 nm and 400 nm with 100 nm having been chosen arbitrarily as the boundary between non-ionizing and ionizing radiation, however EMR is a spectrum and UV can produce molecular ionization. UV radiation is conventionally categorized into 3 areas: UV-A (>315-400 nm),UV-B (>280-315 nm)and UV-C (>100-280 nm) [IARC,Working Group Reports,2005] An important consequence of stratospheric ozone depletion is the increased transmission of solar ultraviolet (UV)radiation to the Earth's lower atmosphere and surface. Stratospheric ozone levels have been falling, in certain areas, for the past several decades, so current surface ultraviolet-B (UV-B) radiation levels are thought to be close to their modern day maximum. [S.Madronich et al.1998] Overexposure of ultraviolet radiation a major cause of skin cancer including basal cell carcinoma (BCC), squamous cell carcinoma (SCC) { collectively referred to as “non-melanoma" skin cancer (NMSC) and melanoma as well, with skin cancers being the most common cancer in North America. [Armstrong et al. 1993, Gallagher et al. 2005] Methods and Experimental Design: Our experiments and testing of a novel UV “Antiradiation Vaccine” have employed a wide variety of laboratory animals which include : Chinchilla

  2. Effects of UV-B radiation on a hereditary suture cataract in mice

    International Nuclear Information System (INIS)

    Forker, Carina; Wegener, Alfred

    1997-01-01

    UV-B (290-320 nm, λ max = 305 nm) radiation and the Cat2 ns (suture cataract) mutation in mice affect both the anterior lens epithelium and the formation of the suture. A low dose of UV-B radiation (2.2 Jcm -2 ) induces similar anterior subcapsular and cortical lens opacities in wild type as in heterozygous mutant mice. The UV-B treatment of the mutant lenses, however, leads to an increase in the number of epithelial cell layers in the anterior central part as compared to the wild type indicating a more severe form of the cataract formation in mutants. In addition, mutants demonstrate a predisposition for a rupture of the posterior lens capsule, because from 2.9 Jcm -2 and higher, this phenomenon could always be observed in the UV-B treated mutants, but never in the treated wild type mice. The protein biochemical analyses were performed by gel electrophoresis and isoelectric focusing of extracts of total lenses or from defined areas of the lens (lens slice technique). These covered the patterns of those proteins already synthesized before irradiation, which in irradiated lenses in no case evidenced a difference to the untreated control, neither in the wild type nor in the mutants. In contrast, by analysing specifically those proteins, which are synthesized after irradiation, in both treated groups a protein with a molecular mass of about 31 kDa becomes discernable in both treated groups. In addition, the cataractous lenses demonstrate a significantly enhanced overall synthesis of water-soluble proteins after irradiation, which might promote the rupture of the posterior capsule at the posterior pole. The present study offers for the first time the possibility to discriminate between endogeneous (genetic) effects and exogeneous (environmental) effects in cataractogenesis and to study their interactive effects. The first set of experiments demonstrated a clear intensification of the hereditary cataract by the UV-B treatment. The study supports the hypothesis that

  3. Risks of increased UV-B radiation for humans

    International Nuclear Information System (INIS)

    Przybilla, B.; Eberlein-Koenig, B.; Bergner, T.

    1994-01-01

    If not compensated in any way, depletion of the stratospheric ozone layer leads to an increase of UV-B radiation at the earth's surface, especially towards the short-wave range, which is biologically the more active. The most concerning effect here is that of UV-B induced skin reactions, in particular malignant skintumors (malignant melanoma, spinocellular carcinoma, basalioma), whose incidence is expected to increase in future. As some photoreactions can be inhibited or enhanced also by radiation outside their action spectrum, it is possible for changes in solar spectral radiation flux density to influence photo-induced reactions that are driven at wavelengths outside the UV-B range. The authors have performed studies for developing methods of quantifying individual UV sensitivity. In vitro studies have shown that UV-A dependent photoreactions can be partly inhibited by UV-B. A number of drugs, as well as sulphites, which are used as preservatives amongst other things, have been shown to have phototoxic properties that may be relevant to photocarcinogenesis. Irradiation tests on cell cultures for different UV-B ranges have shown that irradiation at shorter wavelengths leads to a stronger release of proinflammatory cytokines that ar longer wavelengths with the same dose. Altogether it can be said that despite compelling theoretical evidence it is not easily possible to predict the actual consequences of an increase in particular of short-wave UV-B radiation at the earth's surface. The assumed effects must be examined individually by appropriate methods. (orig.) [de

  4. UV-Radiation: From Physics to Impacts

    Directory of Open Access Journals (Sweden)

    Hanns Moshammer

    2017-02-01

    Full Text Available Ultraviolet (UV radiation has affected life at least since the first life forms moved out of the seas and crawled onto the land. Therefore, one might assume that evolution has adapted to natural UV radiation. However, evolution is mostly concerned with the propagation of the genetic code, not with a long, happy, and fulfilling life. Because rickets is bad for a woman giving birth, the beneficial effects of UV-radiation outweigh the adverse effects like aged skin and skin tumors of various grades of malignancy that usually only afflict us at older age. Anthropogenic damage to the stratospheric ozone layer and frighteningly high rates of melanoma skin cancer in the light-skinned descendants of British settlers in Australia piqued interest in the health impacts of UV radiation. A changing cultural perception of the beauty of tanned versus light skin and commercial interests in selling UV-emitting devices such as tanning booths caught public health experts off-guard. Counseling and health communication are extremely difficult when dealing with a “natural” risk factor, especially when this risk factor cannot (and should not be completely avoided. How much is too much for whom or for which skin type? How even measure “much”? Is it the (cumulative dose or the dose rate that matters most? Or should we even construct a more complex metric such as the cumulative dose above a certain dose rate threshold? We find there are still many open questions, and we are glad that this special issue offered us the opportunity to present many interesting aspects of this important topic.

  5. Effects of UV-B radiation on soybean yield and seed quality: a 6-year field study

    International Nuclear Information System (INIS)

    Teramura, A.H.; Sullivan, J.H.; Lydon, J.

    1990-01-01

    Two soybean [Glycine max (L.) Merr.] cultivars, Essex and Williams, were grown in the field for 6 consecutive seasons under ambient and supplemental levels of ultravio-Set-B radiation to determine the potential for alterations in yield or seed quality with a reduction in the stratospheric ozone column. The supplemental UV-B fluences simulated a 16 or 25% ozone depletion. The data presented here represent the first field experiment conducted over multiple seasons which assesses the effects of increased UV-B radiation on seed yield. Overall, the cultivar Essex was found to be sensitive to UV-B radiation (yield reductions of 20%) while the cultivar Williams was tolerant. However, the effectiveness of UV-B radiation in altering yield was strongly influenced by the seasonal microclimate, and the 2 cultivars responded differently to these changing factors. Yield was reduced most in Essex during seasons in which water availability was high and was reduced in Williams only when water was severely limiting. The results of these experiments demonstrate the necessity for multiple-year experiments and the need to increase our understanding of the interaction between UV-B radiation and other environmental stresses in order to assess the potential consequences of stratospheric ozone depletion. (author)

  6. Effect of far-UV and near-UV radiation on the cell surface charge of the protozoan Tritrichomonas foetus

    Energy Technology Data Exchange (ETDEWEB)

    Silva Filho, F C; Elias, C A; Souza, W de

    1986-05-01

    Cell electrophoresis was used to detect the effect of far-UV or near-UV radiation on the cell surface charge of the pathogenic protozoan Tritrichomonas foetus. Either far-UV or near-UV radiation interfered with the surface charge of T. foetus at fluences which inhibited cell growth by 50%. Both UV-radiations induced a significant decrease on surface charge of T. foetus, as evaluated by measurement of its electrophoretic mobility (EPM). Determinations of EPM of protozoa in solution of low ionic strength indicated that the decrease in the EPM induced by far-UV is much less pronounced that that observed for near-UV or control cells.

  7. Effect of far-UV and near-UV radiation on the cell surface charge of the protozoan Tritrichomonas foetus

    International Nuclear Information System (INIS)

    Silva Filho, F.C.; Elias, C.A.; Souza, W. de

    1986-01-01

    Cell electrophoresis was used to detect the effect of far-UV or near-UV radiation on the cell surface charge of the pathogenic protozoan Tritrichomonas foetus. Either far-UV or near-UV radiation interfered with the surface charge of T. foetus at fluences which inhibited cell growth by 50%. Both UV-radiations induced a significant decrease on surface charge of T. foetus, as evaluated by measurement of its electrophoretic mobility (EPM). Determinations of EPM of protozoa in solution of low ionic strength indicated that the decrease in the EPM induced by far-UV is much less pronounced that that observed for near-UV or control cells. (author)

  8. Effects of stron UV-B radiation on air chemistry and climate; Auswirkungen verstaerkter UV-B-Strahlung auf Luftchemie und Klima

    Energy Technology Data Exchange (ETDEWEB)

    Schoenemeyer, T.; Seidl, W.; Forkel, R.; Kuhn, M.; Wehrhahn, J.; Grell, G.

    1998-07-01

    Effects of enhanced UV radiation on air chemistry, climate and climate change were investigated, and its interactions with other environmental problems like acidification of soil and surface water, reduction in the variety of species, and desertification were gone into. [German] In der vorliegenden Arbeit wurden die bisher vorliegenden Erkenntnisse ueber die Auswirkungen erhoehter UV-Strahlung infolge des Abbaus von Ozon in der Stratosphaere auf Luftchemie und Klima zusammengetragen. Die Problematik wird in ihrer ganzen Breite beleuchtet und dabei deutlich gemacht, ueber welche zahlreichen Mechanismen eine erhoehte UV-Strahlung auch zu Klimaaenderungen fuehren kann. Dies unterstreicht die Notwendigkeit, Verknuepfungen mit anderen Umweltproblemen wie der Versauerung des Bodens und von Gewaessern, der Abnahme der Artenvielfalt sowie der zunehmenden Wuestenbildung herzustellen. (orig.)

  9. UV-B radiation-induced oxidative stress and p38 signaling pathway involvement in the benthic copepod Tigriopus japonicus.

    Science.gov (United States)

    Kim, Bo-Mi; Rhee, Jae-Sung; Lee, Kyun-Woo; Kim, Min-Jung; Shin, Kyung-Hoon; Lee, Su-Jae; Lee, Young-Mi; Lee, Jae-Seong

    2015-01-01

    Ultraviolet B (UV-B) radiation presents an environmental hazard to aquatic organisms. To understand the molecular responses of the intertidal copepod Tigriopus japonicus to UV-B radiation, we measured the acute toxicity response to 96 h of UV-B radiation, and we also assessed the intracellular reactive oxygen species (ROS) levels, glutathione (GSH) content, and antioxidant enzyme (GST, GR, GPx, and SOD) activities after 24 h of exposure to UV-B with LD50 and half LD50 values. Also, expression patterns of p53 and hsp gene families with phosphorylation of p38 MAPK were investigated in UV-B-exposed copepods. We found that the ROS level, GSH content, and antioxidant enzyme activity levels were increased with the transcriptional upregulation of antioxidant-related genes, indicating that UV-B induces oxidative stress by generating ROS and stimulating antioxidant enzymatic activity as a defense mechanism. Additionally, we found that p53 expression was significantly increased after UV-B irradiation due to increases in the phosphorylation of the stress-responsive p38 MAPK, indicating that UV-B may be responsible for inducing DNA damage in T. japonicus. Of the hsp family genes, transcriptional levels of hsp20, hsp20.7, hsp70, and hsp90 were elevated in response to a low dose of UV-B radiation (9 kJ m(-2)), suggesting that these hsp genes may be involved in cellular protection against UV-B radiation. In this paper, we performed a pathway-oriented mechanistic analysis in response to UV-B radiation, and this analysis provides a better understanding of the effects of UV-B in the intertidal benthic copepod T. japonicus. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. Biological responses to current UV-B radiation in Arctic regions

    DEFF Research Database (Denmark)

    Albert, Kristian Rost; Mikkelsen, Teis Nørgaard; Ro-Poulsen, H.

    2008-01-01

    on high-arctic vegetation. They supplement previous investigations from the Arctic focussing on other variables like growth etc., which have reported no or minor plant responses to UV-B, and clearly indicates that UV-B radiation is an important factor affecting plant life at high-arctic Zackenberg......Depletion of the ozone layer and the consequent increase in solar ultraviolet-B radiation (UV-B) may impact living conditions for arctic plants significantly. In order to evaluate how the prevailing UV-B fluxes affect the heath ecosystem at Zackenberg (74°30'N, 20°30'W) and other high......-arctic regions, manipulation experiments with various set-ups have been performed. Activation of plant defence mechanisms by production of UV-B absorbing compounds was significant in ambient UV-B in comparison to a filter treatment reducing the UV-B radiation. Despite the UV-B screening response, ambient UV...

  11. Biological responses to current UV-B radiation in Arctic regions

    DEFF Research Database (Denmark)

    Albert, Kristian Rost; Mikkelsen, Teis Nørgaard; Ro-Poulsen, H.

    2008-01-01

    Depletion of the ozone layer and the consequent increase in solar ultraviolet-B radiation (UV-B) may impact living conditions for arctic plants significantly. In order to evaluate how the prevailing UV-B fluxes affect the heath ecosystem at Zackenberg (74°30'N, 20°30'W) and other high......-arctic regions, manipulation experiments with various set-ups have been performed. Activation of plant defence mechanisms by production of UV-B absorbing compounds was significant in ambient UV-B in comparison to a filter treatment reducing the UV-B radiation. Despite the UV-B screening response, ambient UV...... (mycorrhiza) or in the biomass of microbes in the soil of the root zone. However, the composition of the soil microbial community was different in the soils under ambient and reduced UV radiation after three treatment years. These results provide new insight into the negative impact of current UV-B fluxes...

  12. Ecological responses to UV radiation: interactions between the biological effects of UV on plants and on associated organisms.

    Science.gov (United States)

    Paul, Nigel D; Moore, Jason P; McPherson, Martin; Lambourne, Cathryn; Croft, Patricia; Heaton, Joanna C; Wargent, Jason J

    2012-08-01

    Solar ultraviolet (UV)-B radiation (280-315 nm) has a wide range of effects on terrestrial ecosystems, yet our understanding of how UV-B influences the complex interactions of plants with pest, pathogen and related microorganisms remains limited. Here, we report the results of a series of experiments in Lactuca sativa which aimed to characterize not only key plant responses to UV radiation in a field environment but also consequential effects for plant interactions with a sap-feeding insect, two model plant pathogens and phylloplane microorganism populations. Three spectrally modifying filters with contrasting UV transmissions were used to filter ambient sunlight, and when compared with our UV-inclusive filter, L. sativa plants grown in a zero UV-B environment showed significantly increased shoot fresh weight, reduced foliar pigment concentrations and suppressed population growth of green peach aphid (Myzus persicae). Plants grown under a filter which allowed partial transmission of UV-A radiation and negligible UV-B transmission showed increased density of leaf surface phylloplane microbes compared with the UV-inclusive treatment. Effects of UV treatment on the severity of two plant pathogens, Bremia lactucae and Botrytis cinerea, were complex as both the UV-inclusive and zero UV-B filters reduced the severity of pathogen persistence. These results are discussed with reference to known spectral responses of plants, insects and microorganisms, and contrasted with established fundamental responses of plants and other organisms to solar UV radiation, with particular emphasis on the need for future integration between different experimental approaches when investigating the effects of solar UV radiation. Copyright © Physiologia Plantarum 2011.

  13. Is UV-A radiation a cause of malignant melanoma?

    International Nuclear Information System (INIS)

    Moan, J.

    1994-01-01

    The first action spectrum for cutaneous malignant melanoma was published recently. This spectrum was obtained using the fish Xiphophorus. If the same action spectrum applies to humans, the following statements are true: Sunbathing products (agents to protect against the sun) that absorb UV-B radiation provide almost no protection against cutaneous malignant melanoma. UV-A-solaria are more dangerous than expected so far. If people are determined to use artificial sources of radiation for tanning, they should choose UV-B solaria rather than UV-A-solaria. Fluorescent tubes and halogen lamps may have weak melanomagenic effects. Ozone depletion has almost no effect on the incidence rates of CMM, since ozone absorbs very little UV-A radiation. Sunbathing products which contain UV-A-absorbing compounds or neutral filter (like titanium oxide) provide real protection against cutaneous malignant melanoma, at least if they are photochemically inert. 34 refs., 2 figs

  14. Plant Responses to Increased UV-B Radiation: A Research Project

    Science.gov (United States)

    DAntoni, H. L.; Skiles, J. W.; Armstrong, R.; Coughlan, J.; Daleo, G.; Mayoral, A.; Lawless, James G. (Technical Monitor)

    1994-01-01

    Ozone decrease implies more ultraviolet-B (UV-B) radiation reaching the surface of the Earth. Increased UV-B radiation triggers responses by living organisms. Despite the large potential impacts on vegetation, little is known about UV-B effects on terrestrial ecosystems. Long-term ecological studies are needed to quantify the effects of increased UV radiation on terrestrial ecosystems, asses the risks, and produce reliable data for prediction. Screening pigments are part of one of the protective mechanism in plants. Higher concentrations of screening pigments in leaves may be interpreted as a response to increased UV radiation. If the screening effect is not sufficient, important molecules will be disturbed by incoming radiation. Thus, genetics, photosynthesis, growth, plant and leaf shape and size, and pollen grains may be affected. This will have an impact on ecosystem dynamics, structure and productivity. It is necessary to monitor selected terrestrial ecosystems to permit detection and interpretation of changes attributable to global climate change and depleted ozone shield. The objectives of this project are: (1) To identify and measure indicators of the effects of increased solar UV-B radiation on terrestrial plants; (2) to select indicators with the greatest responses to UV-B exposure; (3) to test, adapt or create ecosystem models that use the information gathered by this project for prediction and to enhance our understanding of the effects of increased UV-B radiation on terrestrial ecosystems. As a first step to achieve these objectives we propose a three-year study of forest and steppe vegetation on the North slope of the Brooks Range (within the Arctic circle, in Alaska), in the Saguaro National Monument (near Tucson, Arizona) and in the forests and steppes of Patagonia (Argentina). We selected (1) vegetation north of the Polar Circle because at 70N there is 8% risk of plant damage due to increased UV-B radiation; (2) the foothills of Catalina Mountains

  15. Protein Kinases and Transcription Factors Activation in Response to UV-Radiation of Skin: Implications for Carcinogenesis

    OpenAIRE

    Laurence A. Marchat; Elena Aréchaga Ocampo; Mavil López Casamichana; Carlos Pérez-Plasencia; César López-Camarillo; Elizbeth Álvarez-Sánchez

    2011-01-01

    Solar ultraviolet (UV) radiation is an important environmental factor that leads to immune suppression, inflammation, photoaging, and skin carcinogenesis. Here, we reviewed the specific signal transduction pathways and transcription factors involved in the cellular response to UV-irradiation. Increasing experimental data supporting a role for p38, MAPK, JNK, ERK1/2, and ATM kinases in the response network to UV exposure is discussed. We also reviewed the participation of NF-?B, AP-1, and NRF2...

  16. Is UV-A radiation a cause of malignant melanoma. Er UV-A aarsak til malignt melanom

    Energy Technology Data Exchange (ETDEWEB)

    Moan, J. (Det Norske Radiumhospital, Oslo (Norway))

    1994-03-01

    The first action spectrum for cutaneous malignant melanoma was published recently. This spectrum was obtained using the fish Xiphophorus. If the same action spectrum applies to humans, the following statements are true: Sunbathing products (agents to protect against the sun) that absorb UV-B radiation provide almost no protection against cutaneous malignant melanoma. UV-A-solaria are more dangerous than expected so far. If people are determined to use artificial sources of radiation for tanning, they should choose UV-B solaria rather than UV-A-solaria. Fluorescent tubes and halogen lamps may have weak melanomagenic effects. Ozone depletion has almost no effect on the incidence rates of CMM, since ozone absorbs very little UV-A radiation. Sunbathing products which contain UV-A-absorbing compounds or neutral filter (like titanium oxide) provide real protection against cutaneous malignant melanoma, at least if they are photochemically inert. 34 refs., 2 figs.

  17. Hope and challenge: the importance of ultraviolet (UV) radiation for cutaneous vitamin D synthesis and skin cancer.

    Science.gov (United States)

    Reichrath, Jörg; Reichrath, Sandra

    2012-01-01

    Abstract Solar ultraviolet (UV)-radiation is the most important environmental risk factor for the development of non-melanoma skin cancer (most importantly basal and squamous cell carcinomas), that represent the most common malignancies in Caucasian populations. To prevent these malignancies, public health campaigns were developed to improve the awareness of the general population of the role of UV-radiation. The requirements of vitamin D is mainly achieved by UV-B-induced cutaneous photosynthesis, and the vitamin D-mediated positive effects of UV-radiation were not always adequately considered in these campaigns; a strict "no sun policy" might lead to vitamin D-deficiency. This dilemma represents a serious problem in many populations, for an association of vitamin D-deficiency and multiple independent diseases has been convincingly demonstrated. It is crucial that guidelines for UV-exposure (e.g. in skin cancer prevention campaigns) consider these facts and give recommendations how to prevent vitamin D-deficiency. In this review, we analyze the present literature to help developing well-balanced guidelines on UV-protection that ensure an adequate vitamin D-status without increasing the risk to develop UV-induced skin cancer.

  18. Plant's adaptive response under UV-B-radiation influence

    International Nuclear Information System (INIS)

    Danil'chenko, O.A.; Grodzinskij, D.M.

    2002-01-01

    Reduction of ozone layer, owing to anthropogenic contamination of an atmosphere results in increase of intensity of UV-radiation and shift of its spectrum in the short-wave side that causes strengthening of various biological effects of irradiation. Consequences of these processes may include increase of injuring of plants and decrease of productivity of agricultural crops to increased UV levels. The important significance in the plant's adaptation to different unfavorable factors has the plant's radioadaptive answer. It has been shown that radioadaptation of plants occurred not only after irradiation with g-radiation in low doses but after UV-rays action . Reaction of radioadaptation it seems to be nonspecific phenomenon in relation to type radiations

  19. Comparison of the dose-effect relationship for UV radiation and ionizing radiation

    International Nuclear Information System (INIS)

    Leenhouts, H.P.; Sijsma, M.J.; Chadwick, K.H.

    1990-06-01

    Ionizing radiation and ultraviolet radiation (UV) are both physical agents with mutagenic and carcinogenic properties. However, there are some basic differences in the fundamental mechanism of their interaction with biological material that may have consequences for risk assessment. In this paper the dose-effect relationships for gamma radiation and UV at cellular level will be used to demonstrate the different radio-biological effectiveness of both agents. The results will be discussed in the framework of a biophysical model, based on the assumption that DNA doublestranded lesions are crucial for the cytotoxic action. After exposure to ionizing radiation, the lesions are fixed immediately following irradiation, but after UV exposure the lethal lesions are recognized only in the next DNA synthesis phase. The combination of this concept with the mechanism of lesion induction and the possibility of repair, leads to different dose and time relationships for the radiation effects of both agents. The possible consequences for risk assessment at low levels will be discussed. (author). 9 refs.; 5 figs

  20. Inhibition of photosystem II by UV-B-radiation

    International Nuclear Information System (INIS)

    Tevini, M.; Pfister, K.

    1985-01-01

    The effect of UV-B-radiation on PSII activity of spinach chloroplasts was analyzed by measuring the integrity of the herbicide-binding protein (HBP 32), by measurement of fluorescence induction in the presence of Diuron (DCMU), and by mathematical analysis of the fluorescence induction curves. It was shown that UV-B inactivates the PSII α-centers but not PSII β-centers. However, the possibility cannot be excluded that in addition the donor site of PSII near the reaction center is attacked by UV-B-radiation. (orig.)

  1. Inhibitory effects of ambient levels of solar UV-A and UV-B radiation in growth of cv. New Red Fire lettuce

    International Nuclear Information System (INIS)

    Krizek, D.T.; Britz, S.J.; Mirecki, R.M.

    1998-01-01

    The influence of solar UV-A and UV-B radiation at Beltsville, MD, USA, on growth of Lactuca sativa L. (cv. New Red Fire lettuce) was examined during early summer of 1996 and 1997. Plants were grown from seed in plastic window boxes covered with Llumar to exclude UV-A and UV-B, polyester to exclude UV-B, or tefzel (1996) or teflon (1997) to transmit UV-A and UV-B radiation. After 31-34 days, plants grown in the absence of solar UV-B radiation (polyester) had 63 and 57% greater fresh weight and dry weight of tops, respectively, and 57, 72 and 47% greater dry weight of leaves, stems and roots, respectively, as compared to those grown under ambient UV-B (tefzel or teflon). Plants protected from UV-A radiation as well (Llumar) showed an additional 43 and 35% increase, respectively, in fresh and dry weight of tops and a 33 and 33% increase, respectively, in dry weight of leaves and stems, but no difference in root biomass over those grown under polyester. Excluding ambient UV-B (polyester) significantly reduced the UV absorbance of leaf extracts at 270, 300 and 330 nm (presumptive flavonoids) and the concentration of anthocyantins at 550 nm as compared to those of leaf extract from plants grown under ambient UV-A and UV-B. Additional removal of ambient UV-A (Llumar) reduced the concentration of anthocyanins, but had no further effect on UV absorbance at 270, 300 or 330 nm. These findings provide evidence that UV-B radiation is more important than UV-A radiation for flavonoid induction in this red-pigmented lettuce cultivar. Although previous workers have obtained decreases in lettuce yield under enhanced UV-B, this is the first evidence for inhibitory effects of solar UV-A and UV-B radiation on lettuce growth. (au)

  2. Inhibitory effects of ambient levels of solar UV-A and UV-B radiation in growth of cv. New Red Fire lettuce

    Energy Technology Data Exchange (ETDEWEB)

    Krizek, D.T.; Britz, S.J.; Mirecki, R.M. [Climate Stress Laboratory, Beltsville, MD (United States)

    1998-05-01

    The influence of solar UV-A and UV-B radiation at Beltsville, MD, USA, on growth of Lactuca sativa L. (cv. New Red Fire lettuce) was examined during early summer of 1996 and 1997. Plants were grown from seed in plastic window boxes covered with Llumar to exclude UV-A and UV-B, polyester to exclude UV-B, or tefzel (1996) or teflon (1997) to transmit UV-A and UV-B radiation. After 31-34 days, plants grown in the absence of solar UV-B radiation (polyester) had 63 and 57% greater fresh weight and dry weight of tops, respectively, and 57, 72 and 47% greater dry weight of leaves, stems and roots, respectively, as compared to those grown under ambient UV-B (tefzel or teflon). Plants protected from UV-A radiation as well (Llumar) showed an additional 43 and 35% increase, respectively, in fresh and dry weight of tops and a 33 and 33% increase, respectively, in dry weight of leaves and stems, but no difference in root biomass over those grown under polyester. Excluding ambient UV-B (polyester) significantly reduced the UV absorbance of leaf extracts at 270, 300 and 330 nm (presumptive flavonoids) and the concentration of anthocyantins at 550 nm as compared to those of leaf extract from plants grown under ambient UV-A and UV-B. Additional removal of ambient UV-A (Llumar) reduced the concentration of anthocyanins, but had no further effect on UV absorbance at 270, 300 or 330 nm. These findings provide evidence that UV-B radiation is more important than UV-A radiation for flavonoid induction in this red-pigmented lettuce cultivar. Although previous workers have obtained decreases in lettuce yield under enhanced UV-B, this is the first evidence for inhibitory effects of solar UV-A and UV-B radiation on lettuce growth. (au) 34 refs.

  3. Risks of increased UV-B radiation: higher plants

    International Nuclear Information System (INIS)

    Rau, W.; Hofmann, H.

    1994-01-01

    The question pursued within the Bavarian climate research programme (BayFORKLIM) in the present context was as follows: Does the fact that UV-B radiation increases with growing site elevation mean that the low sensitivity of predominantly alpine plants compared with that of lowland plants is attributable to their different genetic constitution, possibly as a result of selective pressure and/or de alpine species have a greater capacity to develop protective mechanisms? Pairs and triplets of species belonging to the same genus but occuring at different site elevations were grown from seeds in a greenhouse that is, without UV-B. In order to determine their capacity to adapt to UV-B radiation, some of the plants were additionally exposed to UV-B for 5-6 weeks prior to sensitivity testing. Sensitivity was tested by exposing the plants to additional UV-B of different intensities in test chambers. Visible damage, ranging from light bronzing or yellowing to withering, served as an assessment criterion. Levels of UV-B absorbing substances (phenylpropane species, usually flavonoids) were also measured in these plants. The results obtained permit the following conclusions: The greater UV-B resistance of alpine species compared with that of lowland species of the same genus is not attributable to their genetic constitution but rather to their superior adaptability. Superior resistance is in part due to a greater accumulation of UV-B absorbing substances. Distinct differences in sensitivity between different genera could lead to population shifts within ecosystems as a result of increased UV-B radiation. (orig./KW) [de

  4. The response of human skin commensal bacteria as a reflection of UV radiation: UV-B decreases porphyrin production.

    Directory of Open Access Journals (Sweden)

    Yanhan Wang

    Full Text Available Recent global radiation fears reflect the urgent need for a new modality that can simply determine if people are in a radiation risk of developing cancer and other illnesses. Ultraviolet (UV radiation has been thought to be the major risk factor for most skin cancers. Although various biomarkers derived from the responses of human cells have been revealed, detection of these biomarkers is cumbersome, probably requires taking live human tissues, and varies significantly depending on human immune status. Here we hypothesize that the reaction of Propionibacterium acnes (P. acnes, a human resident skin commensal, to UV radiation can serve as early surrogate markers for radiation risk because the bacteria are immediately responsive to radiation. In addition, the bacteria can be readily accessible and exposed to the same field of radiation as human body. To test our hypothesis, P. acnes was exposed to UV-B radiation. The production of porphyrins in P. acnes was significantly reduced with increasing doses of UV-B. The porphyrin reduction can be detected in both P. acnes and human skin bacterial isolates. Exposure of UV-B to P. acnes- inoculated mice led to a significant decrease in porphyrin production in a single colony of P. acnes and simultaneously induced the formation of cyclobutane pyrimidine dimers (CPD in the epidermal layers of mouse skin. Mass spectrometric analysis via a linear trap quadrupole (LTQ-Orbitrap XL showed that five peptides including an internal peptide (THLPTGIVVSCQNER of a peptide chain release factor 2 (RF2 were oxidized by UV-B. Seven peptides including three internal peptides of 60 kDa chaperonin 1 were de-oxidized by UV-B. When compared to UV-B, gamma radiation also decreased the porphyrin production of P. acnes in a dose-dependent manner, but induced a different signature of protein oxidation/de-oxidation. We highlight that uncovering response of skin microbiome to radiation will facilitate the development of pre

  5. The response of human skin commensal bacteria as a reflection of UV radiation: UV-B decreases porphyrin production.

    Science.gov (United States)

    Wang, Yanhan; Zhu, Wenhong; Shu, Muya; Jiang, Yong; Gallo, Richard L; Liu, Yu-Tsueng; Huang, Chun-Ming

    2012-01-01

    Recent global radiation fears reflect the urgent need for a new modality that can simply determine if people are in a radiation risk of developing cancer and other illnesses. Ultraviolet (UV) radiation has been thought to be the major risk factor for most skin cancers. Although various biomarkers derived from the responses of human cells have been revealed, detection of these biomarkers is cumbersome, probably requires taking live human tissues, and varies significantly depending on human immune status. Here we hypothesize that the reaction of Propionibacterium acnes (P. acnes), a human resident skin commensal, to UV radiation can serve as early surrogate markers for radiation risk because the bacteria are immediately responsive to radiation. In addition, the bacteria can be readily accessible and exposed to the same field of radiation as human body. To test our hypothesis, P. acnes was exposed to UV-B radiation. The production of porphyrins in P. acnes was significantly reduced with increasing doses of UV-B. The porphyrin reduction can be detected in both P. acnes and human skin bacterial isolates. Exposure of UV-B to P. acnes- inoculated mice led to a significant decrease in porphyrin production in a single colony of P. acnes and simultaneously induced the formation of cyclobutane pyrimidine dimers (CPD) in the epidermal layers of mouse skin. Mass spectrometric analysis via a linear trap quadrupole (LTQ)-Orbitrap XL showed that five peptides including an internal peptide (THLPTGIVVSCQNER) of a peptide chain release factor 2 (RF2) were oxidized by UV-B. Seven peptides including three internal peptides of 60 kDa chaperonin 1 were de-oxidized by UV-B. When compared to UV-B, gamma radiation also decreased the porphyrin production of P. acnes in a dose-dependent manner, but induced a different signature of protein oxidation/de-oxidation. We highlight that uncovering response of skin microbiome to radiation will facilitate the development of pre-symptomatic diagnosis

  6. Effect of heat, UV radiation, and moisture on the decohesion kinetics of inverted organic solar cells

    KAUST Repository

    Rolston, Nicholas; Printz, Adam D.; Dupont, Stephanie R.; Voroshazi, Eszter; Dauskardt, Reinhold H.

    2017-01-01

    Organic solar cells subjected to environmental stressors such as heat, moisture, and UV radiation can undergo significant mechanical degradation, leading to delamination of layers and device failure. This paper reports the effect these stressors

  7. Inhibitory effects of ambient levels of solar UV-A and UV-B radiation on growth of cucumber

    International Nuclear Information System (INIS)

    Krizek, D.T.; Mirecki, R.M.; Britz, S.J.

    1997-01-01

    The influence of solar UV-A and UV-B radiation at Beltsville, Maryland, on growth and flavonoid content in four cultivars of Cucumis sativus L. (Ashley, Poinsett, Marketmore, and Salad Bush cucumber) was examined during the summers of 1994 and 1995. Plants were grown from seed in UV exclusion chambers consisting of UV-transmitting Plexiglas, lined with Llumar to exclude UV-A and UV-B, polyester to exclude UV-B, or cellulose acetate to transmit UV-A and UV-B. Despite previously determined differences in sensitivity to supplemental UV-B radiation, all four cultivars responded similarly to UV-B exclusion treatment. After 19–21 days, the four cultivars grown in the absence of solar UV-B (polyester) had an average of 34, 55, and 40% greater biomass of leaves, stems, and roots, respectively, 27% greater stem height, and 35% greater leaf area than those grown under ambient UV-B (cellulose acetate). Plants protected from UV-A radiation as well (Llumar) showed an additional 14 and 22% average increase, respectively, in biomass of leaves and stems, and a 22 and 19% average increase, respectively, in stem elongation and leaf area over those grown under polyester. These findings demonstrate the extreme sensitivity of cucumber not only to present levels of UV-B but also to UV-A and suggest that even small changes in ozone depletion may have important biological consequences for certain plant species. (author)

  8. Effects of combined X-radiation and UV-radiation on HeLa cells

    International Nuclear Information System (INIS)

    Luible, M.

    1982-01-01

    A combined X-ray-UV irradiation was performed in nonsynchronized HeLa-cells. A pre-irradiation with UV-light, that reduced the survival rate to 42% and the following X-ray radiation yielded a similar dose-effect characteristic as with ordinary X-ray irradiation, only its shoulder was smaller. An additive radiation interaction with the cellular molecular structure was observed. A pre-irradiation with X-rays followed by step-wise UV-irradiation yielded a function similar to the UV-action curve but also with a narrower shoulder. A additive effect could be observed. One can conclude from this that in combined irradiation two interacting processes cause the death of the cells. The gene mutations caused by UV-light lead to cell death. X-rays however cause chromosome breaks, that in an unfavourable combination also lead to cell death. The DNA distorsion caused by the UV-light increases the possibility of misrepair. (orig.) [de

  9. Ecological and nonhuman biological effects of solar UV-B radiation

    International Nuclear Information System (INIS)

    Worrest, R.C.

    1984-01-01

    Recent studies regarding the impact of UV-B radiation upon ecological and nonhuman biological systems is the subject of the report. For years scientists and laymen alike have causally noted the impact of solar ultraviolet radiation upon the nonhuman component of the biosphere. Stratospheric ozone functions effectively as an ultraviolet screen by filtering out solar radiation in the 220-320 nm waveband as it penetrates through the atmosphere, thus allowing only small amounts of the longer wavelengths of radiation in the waveband to leak through to the surface of the earth. Although this radiation (UV-B radiation, 290-320 nm) comprises only a small fraction (lesser tha 1%) of the total solar spectrum, it can have a major impact on biological systems due to its actinic nature. Many organic molecules, most notably DNA, absorb UV-B radiation which can initiate photochemical reactions. It is life's ability, or lack thereof, to cope with enhanced levels of solar UV-B radiation that has generated concern over the potential depletion of stratospheric ozone

  10. UV radiation and organic matter composition shape bacterial functional diversity in sediments

    NARCIS (Netherlands)

    Hunting, E.R.; White, C.M.; van Gemert, M.; Mes, D.; Stam, E.; van der Geest, H.G.; Kraak, M.H.S.; Admiraal, W.

    2013-01-01

    UV radiation and organic matter (OM) composition are known to influence the species composition of bacterioplankton communities. Potential effects of UV radiation on bacterial communities residing in sediments remain completely unexplored to date. However, it has been demonstrated that UV radiation

  11. UV-B radiation and photosynthetic irradiance acclimate eggplant for outdoor exposure

    International Nuclear Information System (INIS)

    Latimer, J.G.; Mitchell, G.A.

    1987-01-01

    Treatment of greenhouse-grown eggplant (Solanum melongena L. var. esculentum Nees. 'Burpee's Black Beauty') seedlings with supplemental photosynthetically active radiation from cool-white fluorescent lamps increased growth of plants subsequently transferred outdoors relative to growth of plants that received no supplemental radiation or were shaded to 45% of solar irradiation in the greenhouse before transfer outdoors. Eggplant seedlings transferred outdoors were placed under plastic tarps either to provide relative protection from solar ultraviolet-B (UV-B) radiation (280-315 nm) using Mylar film or to allow exposure to UV-B using cellulose acetate. Protection of seedlings from UV-B radiation resulted in greater leaf expansion than for UV-B-exposed seedlings, but no change in leaf or shoot dry weight occurred after 9 days of treatment. Specific leaf weight increased in response to UV-B exposure outdoors. Exposure of eggplant to UV-B radiation from fluorescent sunlamps in the greenhouse also decreased leaf expansion and leaf and shoot dry weight gain after 5 days of treatment. However, there were no differences in leaf or shoot dry weight relative to control plants after 12 days of UV-B treatment, indicating that UV-B treated plants had acclimated to the treatment and actually had caught up with non-UV-B-irradiated plants in terms of growth

  12. COMPARATIVE ANALYSIS OF UV-C AND UV-B RADIATION INFLUENCE ON PLANT OBJECTS

    Directory of Open Access Journals (Sweden)

    О. Міхєєв

    2011-04-01

    Full Text Available General aim of work – comparative research of temporal regularities of growth processes of pea,that was grown under normal conditions and with application of UV-C and UV-B irradiation ofstem part, and also detection of irradiation dose relations to parameter of root and stem part sproutsgrowth rate of Aronis pea. Research subject of UV-C and UV-B irradiation influence on dynamicsof plant growth parameters in each set of experiments was alteration of growth rate, pecularities ofgrowth dynamics in different conditions of experiment, detection of UV-C and UV-B irradiationdoses range, that stimulate or inhibit growth parameters of pea sprouts. The investigation resulted indetermination 1,3 times higher efficiency of UV-V irradiation comparing to UV-B irradiation.Reaction of root didn’t depend on the type of UV-radiation

  13. UV and EB radiation processing in developing countries

    International Nuclear Information System (INIS)

    Garnett, J.L.

    1991-01-01

    Ultraviolet and electron beams (EB) are to be considered as complementary technologies in the radiation processing field. In many countries, UV processing is used as the pathfinder for EB. In the developing countries the decision to adopt radiation processing techniques to choose between UV and EB will largely be determined by economics, the availability of the chemists and also skilled personnel to service both lines and equipment. (orig./A.B.)

  14. Environmental occurrence and distribution of organic UV stabilizers and UV filters in the sediment of Chinese Bohai and Yellow Seas.

    Science.gov (United States)

    Apel, Christina; Tang, Jianhui; Ebinghaus, Ralf

    2018-04-01

    Organic UV stabilizers and UV filters are applied to industrial materials and cosmetics worldwide. In plastics they prevent photo-induced degradation, while in cosmetics they protect human skin against harmful effects of UV radiation. This study reports on the occurrence and distribution of organic UV stabilizers and UV filters in the surface sediment of the Chinese Bohai and Yellow Seas for the first time. In total, 16 out of 21 analyzed substances were positively detected. Concentrations ranged from sub-ng/g dw to low ng/g dw. The highest concentration of 25 ng/g dw was found for octocrylene (OC) in the Laizhou Bay. In the study area, characteristic composition profiles could be identified. In Korea Bay, the dominating substances were OC and ethylhexyl salicylate (EHS). All other analytes were below their method quantification limit (MQL). Around the Shandong Peninsula, highest concentrations of benzotriazole derivatives were observed in this study with octrizole (UV-329) as the predominant compound, reaching concentrations of 6.09 ng/g dw. The distribution pattern of UV-329 and bumetrizole (UV-326) were related (Pearson correlation coefficient r > 0.98, p « 0.01 around the Shandong Peninsula), indicating an identical input pathway and similar environmental behavior. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Bystander Effect Induced by UV Radiation; why should we be interested? 

    Directory of Open Access Journals (Sweden)

    Maria Widel

    2012-11-01

    Full Text Available The bystander effect, whose essence is an interaction of cells directly subjected to radiation with adjacent non-subjected cells, via molecular signals, is an important component of ionizing radiation action. However, knowledge of the bystander effect in the case of ultraviolet (UV radiation is quite limited. Reactive oxygen and nitrogen species generated by UV in exposed cells induce bystander effects in non-exposed cells, such as reduction in clonogenic cell survival and delayed cell death, oxidative DNA damage and gene mutations, induction of micronuclei, lipid peroxidation and apoptosis. Although the bystander effect after UV radiation has been recognized in cell culture systems, its occurrence in vivo has not been studied. However, solar UV radiation, which is the main source of UV in the environment, may induce in human dermal tissue an inflammatory response and immune suppression, events which can be considered as bystander effects of UV radiation. The oxidative damage to DNA, genomic instability and the inflammatory response may lead to carcinogenesis. UV radiation is considered one of the important etiologic factors for skin cancers, basal- and squamous-cell carcinomas and malignant melanoma. Based on the mechanisms of actions it seems that the UV-induced bystander effect can have some impact on skin damage (carcinogenesis?, and probably on cells of other tissues. The paper reviews the existing data about the UV-induced bystander effect and discusses a possible implication of this phenomenon for health risk. 

  16. Investigation of the use of FXG gel dosimeter for UV radiation detection

    International Nuclear Information System (INIS)

    Bero, M.; Abu Kassem, I.

    2009-02-01

    The radio-chromic chemical radiation dosimeter is well known type of detectors that have been used in the measurement application of radiation dose resulting for ionizing radiation such as γ and X-rays. The detector materials consist of mainly water with added gelatin powder that gives the detector its solid shape. Other chemicals sensitive to radiation are also added to form the Ferrous-sulfate Xylenol-orange Gelatin gel detector (FXG). Ionizing radiation effects appears as an increase in the optical absorbance within a defined range of wavelengths located in the visible region of the light spectrum. These visible changes in the materials optical characteristic as a result of radiation exposure is proportional to the radiation absorbed dose at certain wave lengths. Ultraviolet radiation was found to produce similar effects in the FXG detector materials; hence we suggested studying the UV effects in details. It is known that UV radiation carry relatively high quantum energies big enough to enhance important chemical and biological reactions in some exposed medium. This study examines the most important properties required for the FXG detector to be used as a UV monitoring system that is capable of measuring the absorbed UV radiation dose. The study also works on finding chemical detector structure that is easy to be used for simulating the UV interactions with human body. It has been shown that the optical absorbance of standard size FXG samples increases linearly with the exposure time to UV radiation produced by a sun simulator source, when the beam is filtered to produces exposure similar to that found in nature. However, the UV effects are also influenced by the applied UV radiation spectrum used for irradiation as well as the thicknesses of the FXG materials.(authors)

  17. [Temperature sensitivity of wheat plant respiration and soil respiration influenced by increased UV-B radiation from elongation to flowering periods].

    Science.gov (United States)

    Chen, Shu-Tao; Hu, Zheng-Hua; Li, Han-Mao; Ji, Yu-Hong; Yang, Yan-Ping

    2009-05-15

    Field experiment was carried out in the spring of 2008 in order to investigate the effects of increased UV-B radiation on the temperature sensitivity of wheat plant respiration and soil respiration from elongation to flowering periods. Static chamber-gas chromatography method was used to measure ecosystem respiration and soil respiration under 20% UV-B radiation increase and control. Environmental factors such as temperature and moisture were also measured. Results indicated that supplemental UV-B radiation inhibited the ecosystem respiration and soil respiration from wheat elongation to flowering periods, and the inhibition effect was more obvious for soil respiration than for ecosystem respiration. Ecosystem respiration rates, on daily average, were 9%, 9%, 3%, 16% and 30% higher for control than for UV-B treatment forthe five measurement days, while soil respiration rates were 99%, 93%, 106%, 38% and 10% higher for control than for UV-B treatment. The Q10s (temperature sensitivity coefficients) for plant respiration under control and UV-B treatments were 1.79 and 1.59, respectively, while the Q10s for soil respiration were 1.38 and 1.76, respectively. The Q10s for ecosystem respiration were 1.65 and 1.63 under CK and UV-B treatments, respectively. Supplemental UV-B radiation caused a lower Q10 for plant respiration and a higher Q10 for soil respiration, although no significant effect of supplemental UV-B radiation on the Q10 for ecosystem respiration was found.

  18. The role of solar UV radiation in the ecology of alpine lakes.

    Science.gov (United States)

    Sommaruga, R

    2001-09-01

    Solar ultraviolet radiation (UVR, 290-400 nm) is a crucial environmental factor in alpine lakes because of the natural increase of the UVR flux with elevation and the high water transparency of these ecosystems. The ecological importance of UVR, however, has only recently been recognized. This review, examines the general features of alpine lakes regarding UVR, summarizes what is known about the role of solar UVR in the ecology of alpine lakes, and identifies future research directions. Unlike the pattern observed in most lowland lakes, variability of UV attenuation in alpine lakes is poorly explained by differences in dissolved organic carbon (DOC) concentrations, and depends mainly on optical characteristics (absorption) of the chromophoric dissolved organic matter (CDOM). Within the water column of lakes with low DOC concentrations (0.2-0.4 mg l(-1)), UV attenuation is influenced by phytoplankton whose development at depth (i.e. the deep chlorophyll maximum) causes important changes in UV attenuation. Alpine aquatic organisms have developed a number of strategies to minimize UV damage. The widespread synthesis or bioaccumulation of different compounds that directly or indirectly absorb UV energy is one such strategy. Although most benthic and planktonic primary producers and crustacean zooplankton are well adapted to high intensities of solar radiation, heterotrophic protists, bacteria, and viruses seem to be particularly sensitive to UVR. Understanding the overall impact of UVR on alpine lakes would need to consider synergistic and antagonistic processes resulting from the pronounced climatic warming, which have the potential to modify the UV underwater climate and consequently the stress on aquatic organisms.

  19. Effect of UV radiation and its implications on carotenoid pathway in Bixa orellana L.

    Science.gov (United States)

    Sankari, M; Hridya, H; Sneha, P; George Priya Doss, C; Ramamoorthy, Siva

    2017-11-01

    The current study was undertaken to analyse the effect of short-term UV-B and UV-C radiations in provoking carotenoid biosynthesis in Bixa orellana. Seeds of B. orellana were germinated and exposed to the short term UV pre-treatment under controlled environmental condition for 5days. The UV treated young seedlings response in pigment contents; antioxidant enzyme activity and mRNA gene expression level were analysed. The pigment content such as chlorophyll was increased in both UV-B and UV-C treated seedlings, but the total carotenoid level was decreased when compared to the control seedlings this can be attributed to the plant adaptability to survive in a stressed condition. The β-carotene level was increased in UV-B, and UV-C treated young seedlings. No significant changes have occurred in the secondary pigment such as bixin and ABA. The activity of the antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase was significantly increased in UV-B treated seedlings when compared to the UV-C treated seedlings and control. The mRNA expression of the genes involved in bixin biosynthesis pathways such as DXS, PSY, PDS, LCY-β, LCY-ε, CMT, LCD, ADH and CCD genes showed different expression pattern in UV-B and UV-C treated young seedlings. Further we analysed the gene co-expression network to identify the genes which are mainly involved in carotenoid/bixin biosynthesis pathway. Form our findings the CCD, LCY, PDS, ZDS and PSY showed a close interaction. The result of our study shows that the short term UV-B and UV-C radiations induce pigment content, antioxidant enzyme activity and different gene expression pattern allowing the plant to survive in the oxidative stress condition. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. The effects of UV-B radiation on European heathland species

    International Nuclear Information System (INIS)

    Björn, L.O.; Callaghan, T.V.; Johnsen, I.; Lee, J.A.; Manetas, Y.; Paul, N.D.; Sonesson, M.; Wellburn, A.R.; Coop, D.; Heide-Jørgensen, H.S.; Gehrke, C.; Gwynn-Jones, D.; Johanson, U.; Kyparissis, A.; Levizou, E.; Nikolopoulos, D.; Petropoulou, Y.; Stephanou, M.

    1997-01-01

    The effects of enhanced UV-B radiation on three examples of European shrub-dominated vegetation were studied in situ. The experiments were in High Arctic Greenland, northern Sweden and Greece, and at all sites investigated the interaction of enhanced UV-B radiation (simulating a 15% reduction in the ozone layer) with artificially increased precipitation. The Swedish experiment also involved a study of the interaction between enhanced UV-B radiation and elevated CO 2 (600 ppm). These field studies were supported by an outdoor controlled environment study in the United Kingdom involving modulated enhancement of UV-B radiation in combination with elevated CO 2 (700 ppm). Effects of the treatments on plant growth, morphology, phenology and physiology were measured. The effects observed were species specific, and included both positive and negative responses to the treatments. In general the negative responses to UV-B treatments of up to three growing seasons were small, but included reductions in shoot growth and premature leaf senescence. Positive responses included a marked increase in flowering in some species and a stimulation of some photosynthetic processes. UV-B treatment enhanced the drought tolerance of Pinus pinea and Pinus halepensis by increasing leaf cuticle thickness. In general, there were few interactions between the elevated CO 2 and enhanced UV-B treatments. There was evidence to suggest that although the negative responses to the treatments were small, damage may be increasing with time in some long-lived woody perennials. There was also evidence in the third year of treatments for effects of UV-B on insect herbivory in Vaccinium species. The experiments point to the necessity for long-term field investigations to predict the likely ecological consequences of increasing UV-B radiation. (author)

  1. UV Irradiance Enhancements by Scattering of Solar Radiation from Clouds

    Directory of Open Access Journals (Sweden)

    Uwe Feister

    2015-08-01

    Full Text Available Scattering of solar radiation by clouds can reduce or enhance solar global irradiance compared to cloudless-sky irradiance at the Earth’s surface. Cloud effects to global irradiance can be described by Cloud Modification Factors (CMF. Depending on strength and duration, irradiance enhancements affect the energy balance of the surface and gain of solar power for electric energy generation. In the ultraviolet region, they increase the risk for damage to living organisms. Wavelength-dependent CMFs have been shown to reach 1.5 even in the UV-B region at low altitudes. Ground-based solar radiation measurements in the high Andes region at altitudes up to 5917 m a.s.l showed cloud-induced irradiance enhancements. While UV-A enhancements were explained by cloud scattering, both radiation scattering from clouds and Negative Ozone Anomalies (NOA have been discussed to have caused short-time enhancement of UV-B irradiance. Based on scenarios using published CMF and additional spectroradiometric measurements at a low-altitude site, the contribution of cloud scattering to the UV-B irradiance enhancement in the Andes region has been estimated. The range of UV index estimates converted from measured UV-B and UV-A irradiance and modeled cloudless-sky ratios UV-B/erythemal UV is compatible with an earlier estimate of an extreme UV index value of 43 derived for the high Andes.

  2. UV-B Radiation Induces Root Bending Through the Flavonoid-Mediated Auxin Pathway in Arabidopsis.

    Science.gov (United States)

    Wan, Jinpeng; Zhang, Ping; Wang, Ruling; Sun, Liangliang; Wang, Wenying; Zhou, Huakun; Xu, Jin

    2018-01-01

    Ultraviolet (UV)-B radiation-induced root bending has been reported; however, the underlying mechanisms largely remain unclear. Here, we investigate whether and how auxin and flavonoids are involved in UV-B radiation-induced root bending in Arabidopsis using physiological, pharmacological, and genetic approaches. UV-B radiation modulated the direction of root growth by decreasing IAA biosynthesis and affecting auxin distribution in the root tips, where reduced auxin accumulation and asymmetric auxin distribution were observed. UV-B radiation increased the distribution of auxin on the nonradiated side of the root tips, promoting growth and causing root bending. Further analysis indicated that UV-B induced an asymmetric accumulation of flavonoids; this pathway is involved in modulating the accumulation and asymmetric distribution of auxin in root tips and the subsequent redirection of root growth by altering the distribution of auxin carriers in response to UV-B radiation. Taken together, our results indicate that UV-B radiation-induced root bending occurred through a flavonoid-mediated phototropic response to UV-B radiation.

  3. Effects of UV-B radiation on wax biosynthesis

    International Nuclear Information System (INIS)

    Barnes, J.; Paul, N.; Percy, K.; Broadbent, P.; McLaughlin, C.; Mullineaux, P.; Creissen, G.; Wellburn, A.

    1994-01-01

    Two genotypes of tobacco (Nicotiana tabacum L.) were exposed in controlled environment chambers to three levels of biologically effective ultraviolet-B radiation (UV-B BE ; 280-320nm): 0, 4.54 (ambient) and 5.66 (∼ 25% enhancement) kJ m -2 d -1 . After 28 days, the quantity of wax deposited on leaf surfaces was determined gravimetrically; epicuticular wax chemical composition was determined by capillary gas chromatography with homologue assignments confirmed by gas chromatography-mass spectrometry. Leaf wettability was assessed by measuring the contact angle of water droplets placed on leaf surfaces. Tobacco wax consisted of three major hydrocarbon classes: Straight-chain alkanes (C 27 -C 33 ) which comprised ∼ 59% of the hydrocarbon fraction, containing a predominance of odd-chain alkanes with C 31 as the most abundant homologue; branched-chain alkanes (C 25 -C 32 ) which comprised ∼38% of the hydrocarbon fraction with anteiso 3-methyltriacontane (C 30 ) as the predominant homologue; and fatty acids (C 14 -C 18 ) which comprised ∼ 3% of the wax. Exposure to enhanced UV-B radiation reduced the quantity of wax on the adaxial surface of the transgenic mutant, and resulted in marked changes in the chemical composition of the wax on the exposed leaf surface. Enhanced UV-B decreased the quantity of straight-chain alkanes, increased the quantity of branched-chain alkanes and fatty acids, and resulted in shifts toward shorter straight-chain lengths. Furthermore, UV-B-induced changes in wax composition were associated with increased wettability of tobacco leaf surfaces. Overall, the data are consistent with the view that UV-B radiation has a direct and fundamental effect on wax biosynthesis. Relationships between the physico-chemical nature of the leaf surface and sensitivity to UV-B radiation are discussed. (orig.)

  4. The evolutionary response of plants to increased UV-B radiation: Field studies with Arabidopsis thaliana

    International Nuclear Information System (INIS)

    Trumbull, V.L.; Paige, K.N.

    1995-01-01

    The response of a species to any environmental change is determined by both phenotypic and evolutionary adjustments. To date, the majority of research concerning the response of terrestrial plants to increased UV-B radiation has focused on phenotypic adjustments. Recently we have initiated field studies aimed at assessing genetic variation for UV-B sensitivity within a natural population of Arabidopsis thaliana. This population consists of at least eight discrete genotypes that have been confirmed by RAPD analysis. We used an incomplete block design to assess the impact of UV-B (ambient and ambient + 6 kJ) and PAR (low and high) on these genotypes. The high UV-B treatment caused a significant reduction in fruit number and plant height while the high PAR treatment caused a significant increase in these variables. In addition, there was a marginally significant (p=0.1) UV-B x PAR x maternal line interaction for fruit number, indicating that genetic variation for UV-B sensitivity within this population depends on the PAR environment. The combination of high UV-B and high PAR caused a change in fruit number (relative to the ambient UV-B/high PAR treatment) ranging from an increase of 24% to a decrease of 47%. This range was much smaller in the low PAR treatment. These results indicate the potential for increased UV-B radiation to act as an agent of natural selection within this population

  5. Effects of solar UV radiation on diatom assemblages of the Mediterranean

    International Nuclear Information System (INIS)

    Santas, Regas; Lianou, Charalambia; Haeder, D.-P.

    1996-01-01

    Three UV treatments (PAR; PAR + UVA; PAR + UVA + UVB) were performed by placing different UV-absorbing filters over communities developing on ceramic tiles in a natural marine habitat near Korinthos, Greece. The experiment was repeated at three depths (0.5 m, 1 m, 1.5 m) below the surface of the sea. Differences in community structure due to UV radiation exposure were more pronounced during the early stages of community development. After the first 3 weeks of growth, the productivity of the PAR + UVA + UVB treatment was significantly lower than the PAR + UVA but not than the PAR treatment. This difference did not persist thereafter. At 5 weeks of growth, the productivity at 0.5 m was significantly lower than at 1.0 m. No other significant differences were observed. The findings of the present study suggest that periphytic communities occurring at the upper layers of the euphotic zone may be capable of adjusting to changes in environmental stresses such as by increased solar UVB irradiance. (Author)

  6. UV radiation and organic matter composition shape bacterial functional diversity in sediments

    Directory of Open Access Journals (Sweden)

    Ellard Roy Hunting

    2013-10-01

    Full Text Available AbstractUV radiation and organic matter (OM composition are known to influence the speciescomposition of bacterioplankton communities. Potential effects of UV radiation onbacterial communities residing in sediments remain completely unexplored to date.However, it has been demonstrated that UV radiation can reach the bottom of shallowwaters and wetlands and alter the OM composition of the sediment, suggesting thatUV radiation may be more important for sediment bacteria than previously anticipated.It is hypothesized here that exposure of shallow OMcontaining sediments to UVradiation induces OMsource dependant shifts in the functional composition ofsediment bacterial communities. This study therefore investigated the combinedinfluence of both UV radiation and OM composition on bacterial functional diversity inlaboratory sediments. Two different organic matter sources, labile and recalcitrantorganic matter (OM, were used and metabolic diversity was measured with BiologGN. Radiation exerted strong negative effects on the metabolic diversity in thetreatments containing recalcitrant OM, more than in treatments containing labile OM.The functional composition of the bacterial community also differed significantlybetween the treatments. Our findings demonstrate that a combined effect of UVradiation and OM composition shapes the functional composition of microbialcommunities developing in sediments, hinting that UV radiation may act as animportant sorting mechanism for bacterial communities and driver for bacterialfunctioning in shallow lakes and wetlands.

  7. MicroRNA-22 promotes cell survival upon UV radiation by repressing PTEN

    International Nuclear Information System (INIS)

    Tan, Guangyun; Shi, Yuling; Wu, Zhao-Hui

    2012-01-01

    Highlights: ► miR-22 is induced in cells treated with UV radiation. ► ATM is required for miR-22 induction in response to UV. ► miR-22 targets 3′-UTR of PTEN to repress its expression in UV-treated cells. ► Upregulated miR-22 inhibits apoptosis in cells exposed to UV. -- Abstract: DNA damage response upon UV radiation involves a complex network of cellular events required for maintaining the homeostasis and restoring genomic stability of the cells. As a new class of players involved in DNA damage response, the regulation and function of microRNAs in response to UV remain poorly understood. Here we show that UV radiation induces a significant increase of miR-22 expression, which appears to be dependent on the activation of DNA damage responding kinase ATM (ataxia telangiectasia mutated). Increased miR-22 expression may result from enhanced miR-22 maturation in cells exposed to UV. We further found that tumor suppressor gene phosphatase and tensin homolog (PTEN) expression was inversely correlated with miR-22 induction and UV-induced PTEN repression was attenuated by overexpression of a miR-22 inhibitor. Moreover, increased miR-22 expression significantly inhibited the activation of caspase signaling cascade, leading to enhanced cell survival upon UV radiation. Collectively, these results indicate that miR-22 is an important player in the cellular stress response upon UV radiation, which may promote cell survival via the repression of PTEN expression.

  8. Long-term variations of the UV-B radiation over Central Europe as derived from the reconstructed UV time series

    Directory of Open Access Journals (Sweden)

    J. W. Krzyscin

    2004-04-01

    Full Text Available The daily doses of the erythemally weighted UV radiation are reconstructed for three sites in Central Europe: Belsk-Poland (1966–2001, Hradec Kralove-Czech Republic (1964–2001, and Tõravere-Estonia (1967–2001 to discuss the UV climatology and the long-term changes of the UV-B radiation since the mid 1960s. Various reconstruction models are examined: a purely statistical model based on the Multivariate Adaptive Regression Splines (MARS methodology, and a hybrid model combining radiative transfer model calculations with empirical estimates of the cloud effects on the UV radiation. Modeled long-term variations of the surface UV doses appear to be in a reasonable agreement with the observed ones. A simple quality control procedure is proposed to check the homogeneity of the biometer and pyranometer data. The models are verified using the results of UV observations carried out at Belsk since 1976. MARS provides the best estimates of the UV doses, giving a mean difference between the modeled and observed monthly means equal to 0.6±2.5%. The basic findings are: similar climatological forcing by clouds for all considered stations (~30% reduction in the surface UV, long-term variations in UV monthly doses having the same temporal pattern for all stations with extreme low monthly values (~5% below overall mean level at the end of the 1970s and extreme high monthly values (~5% above overall mean level in the mid 1990s, regional peculiarities in the cloud long-term forcing sometimes leading to extended periods with elevated UV doses, recent stabilization of the ozone induced UV long-term changes being a response to a trendless tendency of total ozone since the mid 1990s. In the case of the slowdown of the total ozone trend over Northern Hemisphere mid-latitudes it seems that clouds will appear as the most important modulator of the UV radiation both in long- and short-time scales over next decades. Key words. Atmospheric composition and structure

  9. Long-term variations of the UV-B radiation over Central Europe as derived from the reconstructed UV time series

    Directory of Open Access Journals (Sweden)

    J. W. Krzyscin

    2004-04-01

    Full Text Available The daily doses of the erythemally weighted UV radiation are reconstructed for three sites in Central Europe: Belsk-Poland (1966–2001, Hradec Kralove-Czech Republic (1964–2001, and Tõravere-Estonia (1967–2001 to discuss the UV climatology and the long-term changes of the UV-B radiation since the mid 1960s. Various reconstruction models are examined: a purely statistical model based on the Multivariate Adaptive Regression Splines (MARS methodology, and a hybrid model combining radiative transfer model calculations with empirical estimates of the cloud effects on the UV radiation. Modeled long-term variations of the surface UV doses appear to be in a reasonable agreement with the observed ones. A simple quality control procedure is proposed to check the homogeneity of the biometer and pyranometer data. The models are verified using the results of UV observations carried out at Belsk since 1976. MARS provides the best estimates of the UV doses, giving a mean difference between the modeled and observed monthly means equal to 0.6±2.5%. The basic findings are: similar climatological forcing by clouds for all considered stations (~30% reduction in the surface UV, long-term variations in UV monthly doses having the same temporal pattern for all stations with extreme low monthly values (~5% below overall mean level at the end of the 1970s and extreme high monthly values (~5% above overall mean level in the mid 1990s, regional peculiarities in the cloud long-term forcing sometimes leading to extended periods with elevated UV doses, recent stabilization of the ozone induced UV long-term changes being a response to a trendless tendency of total ozone since the mid 1990s. In the case of the slowdown of the total ozone trend over Northern Hemisphere mid-latitudes it seems that clouds will appear as the most important modulator of the UV radiation both in long- and short-time scales over next decades.

    Key words. Atmospheric composition and

  10. Effects of solar UV-B radiation on aquatic ecosystems

    Science.gov (United States)

    Häder, D.-P.

    Solar UV degrades dissolved organic carbon photolytically so that they can readily be taken up by bacterioplankton. On the other hand solar UV radiation inhibits bacterioplankton activity. Bacterioplankton productivity is far greater than previously thought and is comparable to phytoplankton primary productivity. According to the "microbial loop hypothesis," bacterioplankton is seen in the center of a food web, having a similar function to phytoplankton and protists. The penetration of UV and PAR into the water column can be measured. Marine waters show large temporal and regional differences in their concentrations of dissolved and particulate absorbing substances. A network of dosimeters (ELDONET) has been installed in Europe ranging from Abisko in Northern Sweden to Gran Canaria. Cyanobacteria are capable of fixing atmospheric nitrogen which is then made available to higher plants. The agricultural potential of cyanobacteria has been recognized as a biological fertilizer for wet soils such as in rice paddies. UV-B is known to impair processes such as growth, survival, pigmentation, motility, as well as the enzymes of nitrogen metabolism and CO 2 fixation. The marine phytoplankton represents the single most important ecosystem on our planet and produces about the same biomass as all terrestrial ecosystems taken together. It is the base of the aquatic food chain and any changes in the size and composition of phytoplankton communities will directly affect food production for humans from marine sources. Another important role of marine phytoplankton is to serve as a sink for atmospheric carbon dioxide. Recent investigations have shown a large sensitivity of most phytoplankton organisms toward solar short-wavelength ultraviolet radiation (UV-B); even at ambient levels of UV-B radiation many organisms seem to be under UV stress. Because of their requirement for solar energy, the phytoplankton dwell in the top layers of the water column. In this near-surface position

  11. Genetic study of resistance to inhibitory effects of UV radiation in rice (Oryza sativa)

    International Nuclear Information System (INIS)

    Sato, T.; Kang, H.S.; Kumagai, T.

    1994-01-01

    Genetic analysis of resistance to the inhibitory effects of UV radiation on growth of rice (Oryza sativa L.) cultivars was carried out. Some experimental plants were grown in visible radiation supplemented with UV radiation containing a large amount of UV-B and a small amount of UV-C in a phytotron, while others were grown without UV radiation. The degree of resistance to UV radiation was estimated in terms of the degree of reduction caused by supplemental UV radiation in the fresh weight of the aboveground plant parts and the chlorophyll content per unit fresh weight. Fresh weight and chlorophyll content in F 2 plants generated by reciprocally crossing cv. Sasanishiki, a cultivar more resistant to UV radiation, and Norin 1, a cultivar less resistant to such radiation exhibited a normal frequency distribution. The heritabilities of these two properties in F 2 plants were low under conditions of non-supplemental UV radiation. Under elevated UV radiation, the F 2 population shifted to the lower range of fresh weight and chlorophyll content, and the means were close to those of Norin 1. The heritabilities of these two properties were the same in the reciprocal crosses, indicating that maternal inheritance was not involved. Inheritance of chlorophyll content per unit fresh weight was further determined in F 3 lines generated by self-fertilizing F 2 plants of Sasanishiki and Norin 1. The results showed that the F 3 population was segregated into three genotypes, namely, resistant homozygotes, segregated heterozygotes and sensitive homozygotes, with a ratio of 1:65:16. It was thus evident that the resistance to the inhibitory effect of elevated UV radiation in these rice plants was controlled by recessive polygenes. (author)

  12. Photomimetic effect of serotonin on yeast cells irradiated by far-UV radiation

    International Nuclear Information System (INIS)

    Fraikin, G.Y.; Strakhovskaya, M.G.; Rubin, L.B.

    1982-01-01

    The effect of serotonin on the survival of far-UV irradiated cells of the yeast Candida guilliermondii was studied. Serotonin was found to have a photomimetic property. Preincubation of cells with serotonin results in protection against far-UV inactivation, whereas the post-radiation treatment with serotonin causes a potentiation of far-UV lethality. Both effects are similar to those produced by near-UV (334 nm) radiation. The observations provide support to the previously proposed idea that photosynthesized serotonin is the underlying cause of the two effects of near-UV radiation, photoprotection and potentiation of far-UV lethality. Experiments with an excision-deficient strain of the yeast Saccharomyces cerevisiae suggest that the effect of serotonin is by its binding to DNA. (author)

  13. Ultraviolet radiation exposure from UV-transilluminators.

    Science.gov (United States)

    Akbar-Khanzadeh, Farhang; Jahangir-Blourchian, Mahdi

    2005-10-01

    UV-transilluminators use ultraviolet radiation (UVR) to visualize proteins, DNA, RNA, and their precursors in a gel electrophoresis procedure. This study was initiated to evaluate workers' exposure to UVR during their use of UV-transilluminators. The levels of irradiance of UV-A, UV-B, and UV-C were determined for 29 UV-transilluminators at arbitrary measuring locations of 6, 25, 62, and 125 cm from the center of the UV-transilluminator's filter surface in the direction of the operator's head. The operators (faculty, research staff, and graduate students) worked within 62 cm of the transilluminators, with most subjects commonly working at time ranged from 1 to 60 min. Actinic hazard (effective irradiance level of UVR) was also determined for three representative UV-transilluminators at arbitrary measuring locations of 2.5, 5, 10, 15, 20, 30, 40, and 50 cm from these sets' filter surface in the direction of the operator's head. The allowable exposure time for these instruments was less than 20 sec within 15 cm, less than 35 sec within 25 cm, and less than 2 min within 50 cm from the UV-transilluminators' filter surface. The results of this study suggest that the use of UV-transilluminators exposes operators to levels of UVR in excess of exposure guidelines. It is recommended that special safety training be provided for the affected employees and that exposure should be controlled by one or the combination of automation, substitution, isolation, posted warning signs, shielding, and/or personal protective equipment.

  14. Effects of UV-B radiation on microcystin production of a toxic strain of Microcystis aeruginosa and its competitiveness against a non-toxic strain

    International Nuclear Information System (INIS)

    Yang, Zhen; Kong, Fanxiang; Shi, Xiaoli; Yu, Yang; Zhang, Min

    2015-01-01

    Highlights: • UV-B radiation showed higher inhibition to non-toxin producing than toxin-producing strains on growth and photosynthetic activity. • Both intracellular and extracellular MC contents decreased markedly under UV-B radiation. • Higher resistance to UV-B radiation helped toxin-producing M. aeruginosa to predominate in the competition. - Abstract: Microcystins (MCs) produced by toxic cyanobacteria pose a health hazard to humans and animals. Some environmental factors can alter the MC concentrations by affecting the abundance of toxin-producing strains in a cyanobacteria population and/or their toxin production. In this study, we designed a monoculture and competition experiment to investigate the impacts of UV-B radiation on MC production and the competition between toxin and non-toxin producing strains of Microcystis aeruginosa. UV-B radiation resulted in higher inhibition of the growth and photosynthetic activity of the non-toxin producing strain relative to that observed for the toxin-producing strain. Both intracellular and extracellular MC contents decreased markedly when the toxin-producing strain was exposed to UV-B radiation. In addition, a quantitative real-time PCR assay revealed that the ratio of toxin-producing M. aeruginosa under UV-B exposure was higher than that under PAR alone at an early stage of the experiment. However, its abundance under UV-B exposure was lower compared with the PAR alone treatment after day 12. Our study demonstrated that UV-B radiation has a great impact on the abundance of the toxin-producing strain in the Microcystis population and their toxin production, which suggests that the fluctuation of UV-B radiation affects the MC level of cyanobacteria blooms

  15. Effects of UV-B radiation on microcystin production of a toxic strain of Microcystis aeruginosa and its competitiveness against a non-toxic strain

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Zhen, E-mail: zhyang@niglas.ac.cn; Kong, Fanxiang, E-mail: fxkong@niglas.ac.cn; Shi, Xiaoli; Yu, Yang; Zhang, Min

    2015-02-11

    Highlights: • UV-B radiation showed higher inhibition to non-toxin producing than toxin-producing strains on growth and photosynthetic activity. • Both intracellular and extracellular MC contents decreased markedly under UV-B radiation. • Higher resistance to UV-B radiation helped toxin-producing M. aeruginosa to predominate in the competition. - Abstract: Microcystins (MCs) produced by toxic cyanobacteria pose a health hazard to humans and animals. Some environmental factors can alter the MC concentrations by affecting the abundance of toxin-producing strains in a cyanobacteria population and/or their toxin production. In this study, we designed a monoculture and competition experiment to investigate the impacts of UV-B radiation on MC production and the competition between toxin and non-toxin producing strains of Microcystis aeruginosa. UV-B radiation resulted in higher inhibition of the growth and photosynthetic activity of the non-toxin producing strain relative to that observed for the toxin-producing strain. Both intracellular and extracellular MC contents decreased markedly when the toxin-producing strain was exposed to UV-B radiation. In addition, a quantitative real-time PCR assay revealed that the ratio of toxin-producing M. aeruginosa under UV-B exposure was higher than that under PAR alone at an early stage of the experiment. However, its abundance under UV-B exposure was lower compared with the PAR alone treatment after day 12. Our study demonstrated that UV-B radiation has a great impact on the abundance of the toxin-producing strain in the Microcystis population and their toxin production, which suggests that the fluctuation of UV-B radiation affects the MC level of cyanobacteria blooms.

  16. Responses of membrane lipid peroxidation and endogenous hormones of soybean seedlings to UV-B radiation and rare earth

    International Nuclear Information System (INIS)

    Yan Shengrong; Yang Chunhe; Zhang Yuequn

    2009-01-01

    [Objective] The aim was to provide strategies for development of rare earth and control of environmental pollution. [Method] Responses of membrane lipid peroxidation and endogenous hormones of soybean seedlings to UV-B radiation and rare earth were studied through hydroponics in laboratory. [Result] The results showed that under irradiation of UV-B(T1-0.15 W/m2 and T2-0.45 W/m2), chlorophyll and indole-3-acetic acid(IAA) contents firstly decreased during the stress phase (1-5d) and then increased during the restoration phase (6-9d) while contents of malonadialdehyde(MDA) and abscisic acid(ABA) gradually increased during the imposition of UV-B radiation (1-5d) and subsequently decreased during recovery from UV-B stress (6-9d) . With adding of La (Ⅲ) with the concentration of 20mg•L-1, the decline/rise trend of chlorophyll, IAA, MDA and ABA contents was slowed down during the stress period while the rise/decline speed was accelerated during the recovery period. [Conclusion] It suggests that the regulation of La (Ⅲ) on membrane lipid peroxidation and endogenous hormones could increase chlorophyll and IAA contents, improve the metabolism of reactive oxygen species (ROS), inhibit membrane lipid peroxidation, decrease the accumulation amount of ABA and alleviate injury of UV-B radiation to soybean seedlings. Further, the protective potential of La (Ⅲ) was better under low UV-B radiation than under high one

  17. Changes of diffuse UV-B radiation on clear sky days

    International Nuclear Information System (INIS)

    Kon, H.; Ichibayashi, R.; Matsuoka, N.

    2004-01-01

    Measurements of global and diffuse UV-B radiation have been carried out in Matsudo City (35.3 deg N, 139.9 deg E), Japan. Forty clear sky days were chosen and the annual variation of global and diffuse UV-B radiation was analyzed. The dependence of the diffuse component on visibility was also examined. The results are summarized as follows. 1. The maximum of daily global UV-B was beyond 40kJrec mE-2 daysup(-1) and was recorded in late July. The maximum of daily diffuse UV-B was recorded in early July. There was a tendency for the diffuse UV-B to be larger than the direct UV-B during a year in Matsudo. 2. The fraction of diffuse UV-B to global UV-B changed a lot each day. The observed minimum value of the fraction during a year was recorded in early August. 3. There was a tendency for the fraction of diffuse UV-B to global UV-B to decrease when visibility increased. 4. The diffuse components that change a lot each day were properly estimated by using the expected minimum fraction and visibility. Key words: Diffuse UV-B, Ultraviolet, UV-B, Visibility

  18. Protective effect of UV-A radiation during acclimation of the photosynthetic apparatus to UV-B treatment

    Czech Academy of Sciences Publication Activity Database

    Štroch, Michal; Materová, Z.; Vrábl, D.; Karlický, Václav; Šigut, Ladislav; Nezval, J.; Špunda, Vladimír

    2015-01-01

    Roč. 96, nov (2015), s. 90-96 ISSN 0981-9428 R&D Projects: GA MŠk(CZ) LO1415; GA MŠk(CZ) LM2010007 Grant - others:EHP(CZ) EHP-CZ02-OV-1-014-2014 Program:CZ02 Institutional support: RVO:67179843 Keywords : barley (Hordeum vulgare L.) * chlorophyll fluorescence * photosynthesis * photosynthetic pigments * UV-A radiation * UV-B radiation Subject RIV: BO - Biophysics Impact factor: 2.928, year: 2015

  19. Design and Fabrication of an Integrated Circuit for Monitoring UV Radiation for Health Physics Applications

    International Nuclear Information System (INIS)

    Nazififard, Mohammad; Faghihi, Reyhaneh; Champiri, Afshin Mahmoudieh; Norov, Enkhbat; Suh, Kune Y.

    2014-01-01

    A particularly important term in the clinical photobiology is the standard erythemal dose (SED), which is a measure of the erythemal effectiveness of a UV exposure. However, both the quality and quantity of the UV radiation are important factors for the UV monitoring. This paper aims to introduce and investigate a UV radiation meter in order to establish its applicability for non-ionizing radiation detection. The ultraviolet (UV) radiation is part of the electromagnetic spectrum. The biological effects of UV radiation vary enormously with wavelength and for this reason the UV spectrum is further divided into three regions: UVA, UVB, and UVC. There is increasing evidence that long wave UV radiation plays a vital role in the pathogenesis of photo-dermatoses such as polymorphous light eruption as well as photo-aging. UVA, UVB, and UVC can all damage collagen fibers and, therefore, accelerate aging of the skin. Both UVA and UVB destroy vitamin A in the skin, which may cause further damage. The quantities of the UV radiation are generally expressed using the radiometric terminology

  20. Design and Fabrication of an Integrated Circuit for Monitoring UV Radiation for Health Physics Applications

    Energy Technology Data Exchange (ETDEWEB)

    Nazififard, Mohammad; Faghihi, Reyhaneh [Kashan Univ., Kashan (Iran, Islamic Republic of); Champiri, Afshin Mahmoudieh [Shahid Chamran Univ., Ahwaz (Iran, Islamic Republic of); Norov, Enkhbat [POSTECH, Pohang (Korea, Republic of); Suh, Kune Y. [Seoul National Univ., Seoul (Korea, Republic of)

    2014-05-15

    A particularly important term in the clinical photobiology is the standard erythemal dose (SED), which is a measure of the erythemal effectiveness of a UV exposure. However, both the quality and quantity of the UV radiation are important factors for the UV monitoring. This paper aims to introduce and investigate a UV radiation meter in order to establish its applicability for non-ionizing radiation detection. The ultraviolet (UV) radiation is part of the electromagnetic spectrum. The biological effects of UV radiation vary enormously with wavelength and for this reason the UV spectrum is further divided into three regions: UVA, UVB, and UVC. There is increasing evidence that long wave UV radiation plays a vital role in the pathogenesis of photo-dermatoses such as polymorphous light eruption as well as photo-aging. UVA, UVB, and UVC can all damage collagen fibers and, therefore, accelerate aging of the skin. Both UVA and UVB destroy vitamin A in the skin, which may cause further damage. The quantities of the UV radiation are generally expressed using the radiometric terminology.

  1. Mechanism of Cuticle Hole Development in Human Hair Due to UV-Radiation Exposure

    Directory of Open Access Journals (Sweden)

    Kazuhisa Maeda

    2018-03-01

    Full Text Available Hair is easily damaged by ultraviolet (UV radiation, bleaching agents or permanent wave treatments, and as damage progresses, hair loses its gloss, develops split ends and breaks. However, the causes of hair damage due to UV radiation have not yet been clarified. We discovered that in one mechanism facilitating damage to wet hair by UV radiation, the unsaturated fatty acids in wet hair produce hydroxy radicals upon exposure to UV radiation, and these radicals produce cuticle holes between the cuticle layers. In wet hair exposed to UV radiation, cuticle holes were produced only between the cuticle layers, whereas when human hair was immersed in a solution containing hydroxy radicals produced by Fenton’s reaction, a random production of cuticle holes was noted. It is thought that hydroxy radicals are produced only between the cuticle layers by exposure to UV radiation, and cuticle holes are formed only in this region because one of the polyunsaturated fatty acids, linoleic acid, with a bis-allyl hydrogen, is found between the cuticle layers.

  2. Water and Wastewater Disinfection with Peracetic Acid and UV Radiation and Using Advanced Oxidative Process PAA/UV

    Directory of Open Access Journals (Sweden)

    Jeanette Beber de Souza

    2015-01-01

    Full Text Available The individual methods of disinfection peracetic acid (PAA and UV radiation and combined process PAA/UV in water (synthetic and sanitary wastewater were employed to verify the individual and combined action of these advanced oxidative processes on the effectiveness of inactivation of microorganisms indicators of fecal contamination E. coli, total coliforms (in the case of sanitary wastewater, and coliphages (such as virus indicators. Under the experimental conditions investigated, doses of 2, 3, and 4 mg/L of PAA and contact time of 10 minutes and 60 and 90 s exposure to UV radiation, the results indicated that the combined method PAA/UV provided superior efficacy when compared to individual methods of disinfection.

  3. Environmental radioactivity and radiation exposure in 2015; Umweltradioaktivitaet und Strahlenbelastung im Jahr 2015

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2017-07-20

    The information of the German Federal Government on the environmental radioactivity and radiation exposure in 2015 covers the following issues: selected topics of radiation protection, natural radiation exposure; civilizing (artificial) radiation exposure: nuclear power plants and other nuclear facilities, uranium mine recultivation, radioactive materials in industry and households, fallout from nuclear weapon testing and reactor accidents; occupational radiation exposure: exposed personnel in nuclear facilities, aviation personnel, radiation accidents; medical radiation exposure: nuclear medical diagnostics and therapy; non-ionizing radiation: electromagnetic fields, UV radiation, optical radiation.

  4. Characterization of ZnO coated polyester fabrics for UV protection

    International Nuclear Information System (INIS)

    Broasca, G.; Borcia, G.; Dumitrascu, N.; Vrinceanu, N.

    2013-01-01

    The textile industry aims to develop fabrics adapted to environmental conditions, in particular to UV radiation. Taking into account the demand for such materials, we prepare an inorganic–organic material, based on ZnO microparticles impregnation of polyester textiles, to perform combined UV-protection properties and high hydrophobicity. Scanning electron microscopy, UV reflectance, Impedance Spectroscopy, contact angle, air permeability, resistance to vapor transfer and tensile strength measurement are used for analysis of the surface and volume properties, related to the performance of the material under environmental conditions, as UV radiation, water and water vapors. The impregnation method ensures a good homogeneity and dispersion of ZnO microparticles into the textile polymeric matrix. The optimum level of impregnation of the fabrics is established to 3–5% ZnO, yielding stable properties, without overloading the fabric. The response of the coated polymer indicates better absorbing the UV radiation and dissipating the surface charge, time stability against UV and higher hydrophobic character, without modification of the mechanical properties, offering enhanced performance and comfort under environmental conditions.

  5. Biological and medical effects of UV radiation on human health

    International Nuclear Information System (INIS)

    Piazena, H.

    1994-01-01

    Effecsts of UV radiation on human health are discussed. UV radiation is taken up through the skin and eyes. In the case of the eyes, the only known effects are damaging ones (e.g. cataracts). Irradiation of the skin, on the other hand, may either have a prophylactic and therapeutic effect or cause health problems if the exposure is too frequent and/or the dose too high. Positive effects are: Stimulation of the vitamin-D-3 synthesis and the autoimmune system, economisation of blood circulation, higher fitness, and the development of a UV protection system in the skin. Negative effects are: UV erythema, disturbances of the unspecific resistance and the immune system, and photocarcinogenesis. (orig.) [de

  6. Hydroxycinnamic acid derivatives in an aquatic liverwort as possible bioindicators of enhanced UV radiation

    Energy Technology Data Exchange (ETDEWEB)

    Arroniz-Crespo, M.; Nunez-Olivera, E. [Universidad de La Rioja, Complejo Cientifico-Tecnologico, Avda. Madre de Dios 51, 26006 Logrono (La Rioja) (Spain); Martinez-Abaigar, J. [Universidad de La Rioja, Complejo Cientifico-Tecnologico, Avda. Madre de Dios 51, 26006 Logrono (La Rioja) (Spain)], E-mail: javier.martinez@unirioja.es

    2008-01-15

    We examined, under laboratory conditions, the physiological responses of the aquatic liverwort Jungermannia exsertifolia subsp. cordifolia to artificially enhanced ultraviolet (UV) radiation for 82 days, especially considering the responses of five hydroxycinnamic acid derivatives. This species lives in mountain streams, where it is exposed to low temperatures and high UV levels, and this combination is believed to increase the adverse effects of UV. Enhanced UV radiation hardly caused any change in several physiological variables indicative of vitality, such as F{sub v}/F{sub m} and chlorophylls/phaeopigments ratio (OD430/OD410). Thus, this liverwort seemed to be tolerant to UV radiation, probably due to the accumulation of three UV-absorbing hydroxycinnamic acid derivatives: p-coumaroylmalic acid, 5''-(7'',8''-dihydroxycoumaroyl)-2-caffeoylmalic acid, and 5''-(7'',8''-dihydroxy-7-O-{beta}-glucosyl-coumaroyl)-2-caffeoylmalic acid. These compounds might serve as bioindicators of enhanced UV radiation. - Several hydroxycinnamic acid derivatives of an aquatic liverwort are induced by enhanced UV radiation and might serve as bioindicators of changes in UV levels.

  7. Effect of UV on building materials in New Zealand

    International Nuclear Information System (INIS)

    Bennett, A.F.

    1993-01-01

    Building materials can be divided into two main classes; organic or polymeric based and inorganic materials. Inorganic materials are in most cases largely unaffected by UV radiation. Many common polymers have bonds sensitive to radiation in the UV region. Absorption of radiation of these wavelengths will lead to excitation of electrons which can lead to isomerisation, chain scissors, cross linking and free radical formation. It is worth noting that the effects of UV radiation are always acting synergistically with other environmental effects. (author). 4 refs., 2 tabs

  8. UV-B Radiation Impacts Shoot Tissue Pigment Composition in Allium fistulosum L. Cultigens

    Directory of Open Access Journals (Sweden)

    Kristin R. Abney

    2013-01-01

    Full Text Available Plants from the Allium genus are valued worldwide for culinary flavor and medicinal attributes. In this study, 16 cultigens of bunching onion (Allium fistulosum L. were grown in a glasshouse under filtered UV radiation (control or supplemental UV-B radiation [7.0 μmol·m−2·s−2 (2.68 W·m−2] to determine impacts on growth, physiological parameters, and nutritional quality. Supplemental UV-B radiation influenced shoot tissue carotenoid concentrations in some, but not all, of the bunching onions. Xanthophyll carotenoid pigments lutein and β-carotene and chlorophylls a and b in shoot tissues differed between UV-B radiation treatments and among cultigens. Cultigen “Pesoenyj” responded to supplemental UV-B radiation with increases in the ratio of zeaxanthin + antheraxanthin to zeaxanthin + antheraxanthin + violaxanthin, which may indicate a flux in the xanthophyll carotenoids towards deepoxydation, commonly found under high irradiance stress. Increases in carotenoid concentrations would be expected to increase crop nutritional values.

  9. Additives in UV and ionising radiation grafting and curing processes

    International Nuclear Information System (INIS)

    Garnett, J.L.; Ng, L.T.; Viengkhou, V.

    1998-01-01

    Full text: Curing of polymers induced by both UV and ionising radiation are now established technologies. Currently both systems are predominantly based on acrylate chemistry. UV processes use photoinitiators to achieve fast polymerisation. In the proposed paper the significance of the occurrence of concurrent grafting with cure will be examined. particularly with respect to the recycling of finished product. Basic studies on grafting initiated by UV and ionising radiation will be discussed. Polar methyl methacrylate (MMA) and non-polar styrene will be used as representative monomers with cellulose and propylene typifying the backbone polymers. The additives chosen for examination in this study are predominantly components used in radiation curing formulations since grafting and curing are known to be mechanically related. The additives used were mineral acid, photoinitiators, vinyl ethers, oligomers, polyfunctional monomers including multifunctional acrylates (MFAs) and methacrylates (MFMAs). For the first time the use of charge transfer complexes in the Mulliken sense as additives in radiation grafting will be discussed. The CT complexes themselves, being monomers, have also been grafted to the above polymers. Recent developments with excimer laser sources for initiating these processes will be discussed, especially the use of non-acrylate chemistry. Excimer laser sources are shown to complement conventional UV and ionising radiation and are photoinitiator free. Mechanisms for the above grafting and curing processes will be outlined

  10. UV Radiation in an Urban Canyon in Southeast Queensland

    Science.gov (United States)

    McKinley, A. R.; Moore, M. R.; Kimlin, M. G.

    2006-12-01

    Ultraviolet radiation (UV) has the possibility to both harm and to benefit human beings when unprotected exposure occurs. After receiving small amounts of UV our bodies begin to synthesise vitamin D, which is essential for maintaining healthy bones, however excessive UV exposure can result in a variety of damaging outcomes ranging from sunburn to skin cancer and cataracts. For this reason it is very important to understand the different environments in which people encounter UV so as to better prepare the public to make smart and healthy sun exposure decisions. Each day more and more people are moving into large cities around the world and spending their time inside the urban canyon, however UV measurements are generally taken at scientific stations in open areas or on top of tall buildings, meaning that at times the environmental characteristics measured may not accurately represent those found at street-level in these highly urbanized areas. Urban canyons are home to both very tall buildings and tropospheric air pollution, each of which reduces the amount of UV reaching street-level. This study measured the varying difference between UV measurements taken at street-level and at a standard UV monitoring site on top of a building outside of the urban canyon. Investigation was conducted in the central business district (CBD) of Brisbane, Australia, which models the CBDs of large cities around the world in that it boasts a great number of tall buildings, including many skyscrapers. Data was collected under clear sky conditions at five different street-level sites in the CBD (on either side of two streets running perpendicular to one another (four sites) and in a public square) and then compared to that obtained on the same day at the Queensland University of Technology's Australian Sun and Health Research Laboratory (ASHRL), which is located 2.5 kilometres outside Brisbane's CBD. Minimum erythemal dose (MED) data was collected at each location and it was found that

  11. A morphological and cytological study of Petunia hybrida exposed to UV-B radiation

    International Nuclear Information System (INIS)

    Staxén, I.; Bornmann, J.F.

    1994-01-01

    The aim of this study was to investigate whether the cytoskeleton, and in particular the microtubular system, is affected by enhanced levels of ultraviolet-B (280–320 nm, 9 kJ m −2 day −1 biologically effective UV-B radiation) radiation in epidermal cells of Petunia x hybrida Vilm, isolated from leaves of plants grown under UV-B radiation and visible light. In addition, morphological changes during development were monitored. In a previous study microtubules were depolymerized and delays in the different stages of the cell cycle were found when protoplasts of Petunia were irradiated with UV-B radiation (Staxén et al. 1993. Protoplasma 173: 70–76). Thus it was of interest to ascertain whether the cytoskeleton would be similarly affected in an intact system. Assuming an effect of UV-B radiation on the microtubular system, we wished to determine whether this could be correlated to concomitant changes in leaf morphology. Plants of Petunia hybrida were grown in greenhouse conditions in the presence or absence of UV-B radiation. During the course of the experiment, samples were taken from young, expanding leaves and from older, fully expanded leaves and prepared for localization and analysis of microtubules from the adaxial epidermal cells. Morphology rather than the cytoskeleton was affected by UV radiation, despite the fact that the epidermal cytoskeleton would most likely be affected, since it is located in the cells which form the first intercepting layer for incident radiation. Morphological changes under UV-B radiation, as compared to those under control conditions, were reflected in earlier flowering and an increase in leaf number. Cell division was thus stimulated as was also evidenced from the increased leaf area. Our results indicate that the number of stomata differentiated on a leaf area basis was not altered although the number of stomata per epidermal cell was reduced. (author)

  12. The mechanisms of protection of antioxidants on Nostoc sphaeroides against UV-B radiation

    Science.gov (United States)

    Wang, G. H.

    UV radiation is one of space harmful factor for earth organisms in space exploration In the present work we studied on the role of antioxidant system in Nostoc sphaeroides K u tz Cyanobacteria and the effects of exogenous antioxidant molecules on its photosynthetic rate under UV-B radiation It was found that UV-B radiation decreased the photosynthetic activity of cyanobacterium but promoted the activity of antioxidant system to protect photosystem II PSII and exogenous antioxidant sodium nitroprusside SNP N-acetylcysteine NAC had an obvious protection on PSII activity under UV-B radiation The activity of SOD Superoxide Dismutase EC 1 15 1 1 CAT Catalase EC 1 11 1 6 POD Peroxidase EC 1 11 1 7 and content of MDA and ASC were improved by 0 5mM and 1mM SNP but 0 1mM SNP decreased the activity of antioxide system Exogenous NAC addition decreased the activity of SOD POD CAT and the content MDA and ASC but exogenous NAC addition increased the content of GSH The results suggested that exogenous SNP and NAC may protect algae by different mechanisms in which SNP maybe play double roles as sources of reactive free radicals or ROS scavengers in formation of algae s protection of PSII under UV-B radiation while NAC does function as antioxidant reagent or precursor of glutathione which could protect PSII directly from UV-B radiation Keyword antioxidant system exogenous or endogenous antioxidant Nostoc sphaeroides photosynthesis UV-B radiation

  13. Synergism between UV-B radiation and pathogen magnifies amphibian embryo mortality in nature

    International Nuclear Information System (INIS)

    Kiesecker, J.M.; Blaustein, R.

    1995-01-01

    Previous research has shown that amphibians have differential sensitivity to ultraviolet-B (UV-B) radiation. In some species, ambient levels of UV-B radiation cause embryonic mortality in nature. The detrimental effects of UV-B alone or with other agents may ultimately affect amphibians at the population level. Here, we experimentally demonstrate a synergistic effect between UV-B radiation and a pathogenic fungus in the field that increases the mortality of amphibian embryos compared with either factor alone. Studies investigating single factors for causes of amphibian egg mortality or population declines may not reveal the complex factors involved in declines

  14. Experimental assessment of cumulative temperature and UV-B radiation effects on Mediterranean plankton metabolism

    KAUST Repository

    Garcia-Corral, Lara S.; Martinez Ayala, Juan; Duarte, Carlos M.; Agusti, Susana

    2015-01-01

    . The oligotrophic waters are already highly transparent, however, exposure of Mediterranean plankton to ultraviolet radiation (UV-B and UV-A) may increase further if the waters become more oligotrophic, thereby, allowing a deeper UV radiation penetration and likely

  15. Are the surgeons safe during UV-A radiation exposure in collagen cross-linking procedure?

    Science.gov (United States)

    Shetty, Rashmi; Shetty, Rohit; Mahendradas, Padmamalini; Shetty, Bhujang K

    2012-02-01

    To quantify the effect of scattered UV-A radiation used in the collagen cross-linking (CXL) procedure and the amount of radiation reaching the surgeon and the surrounding area and to estimate the dampening effect by various protective devices. In this case series, 3 patients [aged 25-30 (±2.5) years] with keratoconus underwent a CXL procedure with UV-A light and riboflavin. Irradiance was measured using a spectrometer (Model USB2000; Ocean Optics, Inc) for various distances from the source, at various angles, and for different durations of radiation. The spectrometer was also used to measure the dampening effect produced by gown, latex gloves, and UV-protective glasses. Maximum UV-A radiation (1.4 × 10(-9) mW/cm(2)) was measured at 2 cm from the limbus, when the probe was held at a 45-degree angle to the floor. UV-A radiation reaching the surgeon's eye and the abdomen was 3.403 × 10(-11) and 2.36 × 10(-11) mW/cm(2), respectively. Gown, latex gloves, and UV-protective glasses showed dampening effects of 99.58%, 95.01%, and 99.73%, respectively. CXL appears to be a safe procedure with respect to UV-A radiation exposure to the surgeon. Further safety can be ensured by UV-protective devices.

  16. Reduction of patulin in apple cider by UV radiation.

    Science.gov (United States)

    Dong, Qingfang; Manns, David C; Feng, Guoping; Yue, Tianli; Churey, John J; Worobo, Randy W

    2010-01-01

    The presence of the mycotoxin patulin in processed apple juice and cider presents a continual challenge to the food industry as both consumer health and product quality issues. Although several methods for control and/or elimination of patulin have been proposed, no unifying method has been commercially successful for reducing patulin burdens while maintaining product quality. In the present study, exposure to germicidal UV radiation was evaluated as a possible commercially viable alternative for the reduction and possible elimination of the patulin mycotoxin in fresh apple cider. UV exposure of 14.2 to 99.4 mJ/cm(2) resulted in a significant and nearly linear decrease in patulin levels while producing no quantifiable changes in the chemical composition (i.e., pH, Brix, and total acids) or organoleptic properties of the cider. For the range of UV doses tested, patulin levels decreased by 9.4 to 43.4%; the greatest reduction was achieved after less than 15 s of UV exposure. The method of UV radiation (the CiderSure 3500 system) is an easily implemented, high-throughput, and cost-effective method that offers simultaneous UV pasteurization of cider and juice products and reduction and/or elimination of patulin without unwanted alterations in the final product.

  17. Secondary UV radiation from biota as a proof of radiation hormesis and Gurwitsch phenomena

    International Nuclear Information System (INIS)

    Goraczko, W.

    1997-01-01

    High (large) and low (small) doses of ionizing radiation consistently induce opposite physiologic effects in biological systems. The effects of low doses cannot be inferred by interpolation between the result from groups exposed to high doses and controls irradiated only by Natural Background Radiation. Stimulation NBR ('bio-positive') effects by low-level doses of ionizing radiation is called radiation hormesis. It is still a controversial idea; however it was found that some biological objects (yeast, sees, animals) after γ-irradiation by low-level doses (10-50 times more NBR) can increase their development. The results of the researches demonstrate that the excitation of living systems by ionizing radiation (high energy, low doses) produces among other hydrogen peroxide which initiates prolonged secondary emission that can influence biota and activate many important processes in biological systems. On the other hand it is well known that after water irradiation by ionizing radiation as the product of radiolysis concentration of hydrogen peroxide has been received. The spectral analysis of this secondary emission confirmed the contribution of the UV component to the total emission. This secondary radiation can play a very important role in the intercellular communication. The influence of hydrogen peroxide on glycine has been examined. I have measured secondary emission from Gly using the Single Photon Counting device SPC. The data obtained made possible at least a partial understanding of the radiation hormesis phenomenon and suggest closer relationship to mitogenetic radiation. I propose deexcitation processes in biomolecules as a common denominator of UV and ionizing radiation interacting with living cells, underlying both radiation hormesis and mitogenetic effect. Based on the above experiments and other authors' reports it is postulated that low-level doses of ionizing radiation through radiolysis products (among others hydrogen peroxide) generate UV

  18. Growth enhancement of soybean (Glycine max) upon exclusion of UV-B and UV-B/A components of solar radiation: characterization of photosynthetic parameters in leaves.

    Science.gov (United States)

    Guruprasad, Kadur; Kadur, Guruprasad; Bhattacharjee, Swapan; Swapan, Bhattacharjee; Kataria, Sunita; Sunita, Kataria; Yadav, Sanjeev; Sanjeev, Yadav; Tiwari, Arjun; Arjun, Tiwari; Baroniya, Sanjay; Sanjay, Baroniya; Rajiv, Abhinav; Abhinav, Rajiv; Mohanty, Prasanna

    2007-01-01

    Exclusion of UV (280-380 nm) radiation from the solar spectrum can be an important tool to assess the impact of ambient UV radiation on plant growth and performance of crop plants. The effect of exclusion of UV-B and UV-A from solar radiation on the growth and photosynthetic components in soybean (Glycine max) leaves were investigated. Exclusion of solar UV-B and UV-B/A radiation, enhanced the fresh weight, dry weight, leaf area as well as induced a dramatic increase in plant height, which reflected a net increase in biomass. Dry weight increase per unit leaf area was quite significant upon both UV-B and UV-B/A exclusion from the solar spectrum. However, no changes in chlorophyll a and b contents were observed by exclusion of solar UV radiation but the content of carotenoids was significantly (34-46%) lowered. Analysis of chlorophyll (Chl) fluorescence transient parameters of leaf segments suggested no change in the F v/F m value due to UV-B or UV-B/A exclusion. Only a small reduction in photo-oxidized signal I (P700+)/unit Chl was noted. Interestingly the total soluble protein content per unit leaf area increased by 18% in UV-B/A and 40% in UV-B excluded samples, suggesting a unique upregulation of biosynthesis and accumulation of biomass. Solar UV radiation thus seems to primarily affect the photomorphogenic regulatory system that leads to an enhanced growth of leaves and an enhanced rate of net photosynthesis in soybean, a crop plant of economic importance. The presence of ultra-violet components in sunlight seems to arrest carbon sequestration in plants.

  19. UV and vacuum-UV biological spectroscopy using synchrotron radiation

    International Nuclear Information System (INIS)

    Ito, Amando Siuiti

    1996-01-01

    Full text. Synchrotron radiation has been used as light source in the UV and VUV region for the study of many biological systems. In the time domain, measurements are made that allow the observation of dynamics and kinetics of biomolecules like proteins and peptides, using the fluorescent properties of either intrinsic or extrinsic probes. Optical activity of groups inside biomolecules allows the use of circular dichroism techniques to generate structural information and to follow processes like protein folding. Confocal scanning of synchrotron light generates microscopy resolution below 100 nm, allowing the creation of high quality three dimensional images of biological samples, and the collection of fluorescence originated from microvolumes inside the samples. We propose a station at LNLS for these three techniques: time-resolved fluorescence, circular dischroism and confocal microscopy, using UV and VUV light. (author)

  20. Raman spectroscopic analysis of the responds of desert cyanobacterium Nostoc sp under UV-B radiation

    Science.gov (United States)

    Wang, Gaohong; Hao, Zongjie; Hu, Chunxiang; Liu, Yongding

    Cyanobacteria are renowned for tolerating extremes of desiccation, UV radiation, freezethaw cycles, hypersalinity and oligotrophy, which make them as candidate par excellence for terraforming in extraterrestrial planet. Recently Raman spectrum was applied to study the biochemical information changes in different field of life science. In this study, we investigated the respond of desert cyanobactreium Nostoc sp under UV-B radiation via FT-Raman spectra. It was found that the spectral biomarkers of protectant molecular of UV radiation such as β-carotene and scytonemin were induced by UV-B radiation, but Chlorophyll a content was decreased, and also the photosynthesis activity was inhibited significantly. After light adaptation without UV-B radiation, the Chlorophyll a content and photosynthesis activity returned to high level, butβ-carotene and scytonemin content remained in the cells. Those results indicated that desert Cyanobacteria have good adaptation ability for UV-B radiation and synthesis of protectant molecular may be an effective strategy for its adaptation in evolution.

  1. Evaluation of UV-permeability and photo-oxidisability of organic ultraviolet radiation-absorbing coatings

    Energy Technology Data Exchange (ETDEWEB)

    Li, Neng; Chen, Yuhe, E-mail: yuhec@sina.com; Bao, Yongjie; Zhang, Zeqian; Wu, Zaixing; Chen, Zhangmin

    2015-03-30

    Highlights: • We investigate organic UV radiation-absorbing coatings for use on bamboo surfaces. • The size of glass exactly inserted into sample cell of UV-Vis spectrophotometer. • A model was made to predict UV absorption of coatings. • We examine carbonyl groups change of coatings after ageing. • Two formulations which could effectively protect coating were obtained. - Abstract: Enhancing the durability of the coatings used on bamboo products is essential for increasing their use in outdoor environments. In this study, we investigated organic UV radiation-absorbing coatings for use on bamboo surfaces. The degree of resistance of the coatings, which contained 2-(2-hydroxy-3-tert-butyl-5-methyl-phenyl)-5-chlorinated benzotriazole (BTZ-1), to UV radiation degradation was determined through spectroscopic analysis. The critical BTZ-1 loading amount was determined by analysing the spectroscopic data. Fourier transform infrared spectroscopy was used to elucidate the relationship between the degree of photooxidation of the coatings and their BTZ-1 concentration. The experimental results showed that the coatings provided a high degree of shielding from UV radiation. The critical loading amount was determined to be 1.82 ± 0.05 g BTZ-1/m{sup 2}. The coatings formed using the formulations that contained 3 and 5 wt% BTZ-1 exhibited the lowest degree of photooxidation after exposure to UV radiation.

  2. Extensive reduction of surface UV radiation since 1750 in world's populated regions

    Directory of Open Access Journals (Sweden)

    M. M. Kvalevåg

    2009-10-01

    Full Text Available Human activity influences a wide range of components that affect the surface UV radiation levels, among them ozone at high latitudes. We calculate the effect of human-induced changes in the surface erythemally weighted ultra-violet radiation (UV-E since 1750. We compare results from a radiative transfer model to surface UV-E radiation for year 2000 derived by satellite observations (from Total Ozone Mapping Spectroradiometer and to ground based measurements at 14 sites. The model correlates well with the observations; the correlation coefficients are 0.97 and 0.98 for satellite and ground based measurements, respectively. In addition to the effect of changes in ozone, we also investigate the effect of changes in SO2, NO2, the direct and indirect effects of aerosols, albedo changes and aviation-induced contrails and cirrus. The results show an increase of surface UV-E in polar regions, most strongly in the Southern Hemisphere. Furthermore, our study also shows an extensive surface UV-E reduction over most land areas; a reduction up to 20% since 1750 is found in some industrialized regions. This reduction in UV-E over the industrial period is particularly large in highly populated regions.

  3. Near UV radiation effect on the lens and retina

    International Nuclear Information System (INIS)

    Zigman, S.

    1987-01-01

    The discussion presented in this paper indicates that the retina of a diurnal animal with a natural UV-absorbing lens (ie: the gray squirrel) is susceptible to near-UV damage from environmental sources only after the lens has been removed. This suggests that it is very important to protect against near-UV exposure of human eyes after cataract surgery

  4. Reconstruction of solar spectral surface UV irradiances using radiative transfer simulations.

    Science.gov (United States)

    Lindfors, Anders; Heikkilä, Anu; Kaurola, Jussi; Koskela, Tapani; Lakkala, Kaisa

    2009-01-01

    UV radiation exerts several effects concerning life on Earth, and spectral information on the prevailing UV radiation conditions is needed in order to study each of these effects. In this paper, we present a method for reconstruction of solar spectral UV irradiances at the Earth's surface. The method, which is a further development of an earlier published method for reconstruction of erythemally weighted UV, relies on radiative transfer simulations, and takes as input (1) the effective cloud optical depth as inferred from pyranometer measurements of global radiation (300-3000 nm); (2) the total ozone column; (3) the surface albedo as estimated from measurements of snow depth; (4) the total water vapor column; and (5) the altitude of the location. Reconstructed daily cumulative spectral irradiances at Jokioinen and Sodankylä in Finland are, in general, in good agreement with measurements. The mean percentage difference, for instance, is mostly within +/-8%, and the root mean square of the percentage difference is around 10% or below for wavelengths over 310 nm and daily minimum solar zenith angles (SZA) less than 70 degrees . In this study, we used pseudospherical radiative transfer simulations, which were shown to improve the performance of our method under large SZA (low Sun).

  5. Acrylated Palm Oil Resins for Radiation (UV) Curing of Over Print Varnish (OPV)

    International Nuclear Information System (INIS)

    Norshamsul Arif; Mohd Hilmi Mahmood; Khairul Zaman Mohd Dahlan; Rosley Che Ismail; Flora Sim; Sharilla Muhammad Faizal

    2010-01-01

    The purpose of this project was to determine the effects of Acrylated Epoxidized Palm Oil (EPOLA) on a radiation (UV) curing Over Print Varnish (OPV) application. The initial target was to produce an environmentally friendly resin (non-VOC) and reduce the dependence on petroleum (hydrocarbon) based products which are more toxic. Toxicity was determined via Acute Oral Toxicity (LD50), OECD 401 and Acute Toxicity (Dermal), OECD 402 technique whilst the reactivity, chemical resistance and other physical properties were obtained from actual printing application test. The developed EPOLA resin was combined with acrylated monomers, photo initiator(s) and additives in typical OPV formulation before being chemically converted into protective glossy printed film under Ultra Violet (UV-C) light. The gloss, flexibility and adhesion effects are significantly greater than conventional OPV epoxy based coupled with further extremely low irritancy to skin. The contributions of this project are twofold. First, the toxicity of developed acrylated Palm Oil resin is certainly lower than conventional epoxy acrylates resin. Secondly, the benefits towards radiation curing OPV applications were significantly demonstrated. (author)

  6. Experimental assessment of cumulative temperature and UV-B radiation effects on Mediterranean plankton metabolism

    Directory of Open Access Journals (Sweden)

    Lara S. eGarcia-Corral

    2015-07-01

    Full Text Available The Mediterranean Sea is a vulnerable region for climate change, warming at higher rates compare to the global ocean. Warming leads to increased stratification of the water column and enhanced the oligotrophic nature of the Mediterranean Sea. The oligotrophic waters are already highly transparent, however, exposure of Mediterranean plankton to ultraviolet radiation (UV-B and UV-A may increase further if the waters become more oligotrophic, thereby, allowing a deeper UV radiation penetration and likely enhancing impacts to biota.Here we experimentally elucidate the cumulative effects of warming and natural UV-B radiation on the net community production (NCP of plankton communities. We conducted five experiments at monthly intervals, from June to October 2013, and evaluated the responses of NCP to ambient UV-B radiation and warming (+3ºC, alone and in combination, in a coastal area of the northwest Mediterranean Sea. UV-B radiation and warming lead to reduced net community production and resulted in a heterotrophic (NCP<0 metabolic balance. Both UV-B radiation and temperature, showed a significant individual effect in NCP across treatments and time. However, their joint effect showed to be synergistic as the interaction between them (UV x Temp was statistically significant in most of the experiments performed. Our results showed that both drivers, would affect the gas exchange of CO2-O2 from and to the atmosphere and the role of plankton communities in the Mediterranean carbon cycle

  7. Experimental assessment of cumulative temperature and UV-B radiation effects on Mediterranean plankton metabolism

    KAUST Repository

    Garcia-Corral, Lara S.

    2015-07-07

    The Mediterranean Sea is a vulnerable region for climate change, warming at higher rates compare to the global ocean. Warming leads to increased stratification of the water column and enhanced the oligotrophic nature of the Mediterranean Sea. The oligotrophic waters are already highly transparent, however, exposure of Mediterranean plankton to ultraviolet radiation (UV-B and UV-A) may increase further if the waters become more oligotrophic, thereby, allowing a deeper UV radiation penetration and likely enhancing impacts to biota. Here we experimentally elucidate the cumulative effects of warming and natural UV-B radiation on the net community production (NCP) of plankton communities. We conducted five experiments at monthly intervals, from June to October 2013, and evaluated the responses of NCP to ambient UV-B radiation and warming (+3°C), alone and in combination, in a coastal area of the northwest Mediterranean Sea. UV-B radiation and warming lead to reduced NCP and resulted in a heterotrophic (NCP < 0) metabolic balance. Both UV-B radiation and temperature, showed a significant individual effect in NCP across treatments and time. However, their joint effect showed to be synergistic as the interaction between them (UV × Temp) was statistically significant in most of the experiments performed. Our results showed that both drivers, would affect the gas exchange of CO2−O2 from and to the atmosphere and the role of plankton communities in the Mediterranean carbon cycle.

  8. Effect of UV Radiation by Projectors on 3D Printing

    Directory of Open Access Journals (Sweden)

    Kovalenko Iaroslav

    2017-01-01

    Full Text Available Polymers that solidify under light radiation are commonly used in digital light processing (DLP 3D printing. A wide range of photopolymers use photoinitiators that react to radiation in range of ultraviolet (UV wavelength. In the present study we provided measurement of radiant fluence in the UV wavelength range from 280 nm to 400 nm for two data projectors and compared effect of radiation on quality of 3D printing. One projector is commonly used DLP projector with high energy lamp. Second one is an industrial projector, in which RGB light emitting diodes (LEDs are replaced by UV LEDs with wattage at the level of 3.6 % of the first one. Achieved data confirmed uneven distribution of radiant energy on illuminated area. These results validate, that undesired heating light causes internal stress inside built models that causes defects in final products.

  9. Influence of UV-radiation on granulocytic phagocytosis in vitro

    International Nuclear Information System (INIS)

    Walther, T.; Rytter, M.; Gast, W.; Haustein, U.F.

    1987-01-01

    The influence of UV radiation on the vitality, the performance of phagocytosis and the ability to reduce nitro-blue tetrazolium test (NBT) by human granulocytes was investigated in vitro. Already by low doses of UVA (8% UVB) the percentage of phagocytizing granulocytes was decreased more distinctly than their cell vitality. The number of ingested Candida albicans particles was 4.5 particles per granulocyte in the controls. It was reduced to about 1.4 particles per cell by UV radiation independent of the dosis applied. On the other hand the ability of granulocytes to reduce NBT intracellularly remained completely unchanged. (author)

  10. Co-ordinated ozone and UV project COZUV

    International Nuclear Information System (INIS)

    Braathen, Geir

    1999-01-01

    The project encompasses all the major Norwegian research groups in the field of stratospheric ozone and UV research. the duration is from the 1st January 1999 to the 31st December 2000. The tasks carried out will include investigations of the ozone layer over the North Polar and middle latitudes, 3-D chemical modelling, diagnosis of chemical ozone loss, investigations of transport mechanisms between the polar vortex and middle latitudes, study of the coupling between ozone change and climate change in the stratosphere and upper troposphere, scenario calculations in order to investigate the consequences of temperature change in the stratosphere, development of methods to measure global, direct and radiance distribution of UV, to improve UV dose calculations, investigate the influence of clouds on the surface UV radiation and to use existing surface UV radiation measurements together with existing radiation models to investigate the connection between UV radiation and ozone, clouds and surface albedo. The results will be published in various publications, progress reports, by participation in international conferences, through information to the environmental authorities and through information on the Internet

  11. Attenuated UV Radiation Alters Volatile Profile in Cabernet Sauvignon Grapes under Field Conditions.

    Science.gov (United States)

    Liu, Di; Gao, Yuan; Li, Xiao-Xi; Li, Zheng; Pan, Qiu-Hong

    2015-09-17

    This study aimed to explore the effect of attenuated UV radiation around grape clusters on the volatile profile of Cabernet Sauvignon grapes (Vitis vinifera L. cv.) under field conditions. Grape bunches were wrapped with two types of polyester films that cut off 89% (film A) and 99% (film B) invisible sunlight of less than 380 nm wavelength, respectively. Solar UV radiation reaching the grape berry surface was largely attenuated, and an increase in the concentrations of amino acid-derived benzenoid volatiles and fatty acid-derived esters was observed in the ripening grapes. Meanwhile, the attenuated UV radiation significantly reduced the concentrations of fatty acid-derived aldehydes and alcohols and isoprenoid-derived norisoprenoids. No significant impact was observed for terpenes. In most case, these positive or negative effects were stage-dependent. Reducing UV radiation from the onset of veraison to grape harvest, compared to the other stages, caused a larger alteration in the grape volatile profile. Partial Least Square Discriminant Analysis (PLS-DA) revealed that (E)-2-hexenal, 4-methyl benzaldehyde, 2-butoxyethyl acetate, (E)-2-heptenal, styrene, α-phenylethanol, and (Z)-3-hexen-1-ol acetate were affected most significantly by the attenuated UV radiation.

  12. Attenuated UV Radiation Alters Volatile Profile in Cabernet Sauvignon Grapes under Field Conditions

    Directory of Open Access Journals (Sweden)

    Di Liu

    2015-09-01

    Full Text Available This study aimed to explore the effect of attenuated UV radiation around grape clusters on the volatile profile of Cabernet Sauvignon grapes (Vitis vinifera L. cv. under field conditions. Grape bunches were wrapped with two types of polyester films that cut off 89% (film A and 99% (film B invisible sunlight of less than 380 nm wavelength, respectively. Solar UV radiation reaching the grape berry surface was largely attenuated, and an increase in the concentrations of amino acid-derived benzenoid volatiles and fatty acid-derived esters was observed in the ripening grapes. Meanwhile, the attenuated UV radiation significantly reduced the concentrations of fatty acid-derived aldehydes and alcohols and isoprenoid-derived norisoprenoids. No significant impact was observed for terpenes. In most case, these positive or negative effects were stage-dependent. Reducing UV radiation from the onset of veraison to grape harvest, compared to the other stages, caused a larger alteration in the grape volatile profile. Partial Least Square Discriminant Analysis (PLS-DA revealed that (E-2-hexenal, 4-methyl benzaldehyde, 2-butoxyethyl acetate, (E-2-heptenal, styrene, α-phenylethanol, and (Z-3-hexen-1-ol acetate were affected most significantly by the attenuated UV radiation.

  13. Different atmospheric parameters influence on spectral UV radiation (measurements and modelling)

    Energy Technology Data Exchange (ETDEWEB)

    Chubarova, N Y [Moscow State Univ. (Russian Federation). Meteorological Observatory; Krotkov, N A [Maryland Univ., MD (United States). JCESS/Meteorology Dept.; Geogdzhaev, I V; Bushnev, S V; Kondranin, T V [SUMGF/MIPT, Dolgoprudny (Russian Federation); Khattatov, V U [Central Aerological Observatory, Dolgoprudny (Russian Federation)

    1996-12-31

    The ultraviolet (UV) radiation plays a vital role in the biophysical processes despite its small portion in the total solar flux. UV radiation is subject to large variations at the Earth surface depending greatly on solar elevation, ozone and cloud amount, aerosols and surface albedo. The analysis of atmospheric parameters influence is based on the spectral archive data of three spectral instruments: NSF spectroradiometer (Barrow network) (NSF Polar Programs UV Spectroradiometer Network 1991-1992,1992), spectrophotometer (SUVS-M) of Central Aerological Observatory CAO, spectroradiometer of Meteorological Observatory of the Moscow State University (MO MSU) and model simulations based on delta-Eddington approximation

  14. Different atmospheric parameters influence on spectral UV radiation (measurements and modelling)

    Energy Technology Data Exchange (ETDEWEB)

    Chubarova, N.Y. [Moscow State Univ. (Russian Federation). Meteorological Observatory; Krotkov, N.A. [Maryland Univ., MD (United States). JCESS/Meteorology Dept.; Geogdzhaev, I.V.; Bushnev, S.V.; Kondranin, T.V. [SUMGF/MIPT, Dolgoprudny (Russian Federation); Khattatov, V.U. [Central Aerological Observatory, Dolgoprudny (Russian Federation)

    1995-12-31

    The ultraviolet (UV) radiation plays a vital role in the biophysical processes despite its small portion in the total solar flux. UV radiation is subject to large variations at the Earth surface depending greatly on solar elevation, ozone and cloud amount, aerosols and surface albedo. The analysis of atmospheric parameters influence is based on the spectral archive data of three spectral instruments: NSF spectroradiometer (Barrow network) (NSF Polar Programs UV Spectroradiometer Network 1991-1992,1992), spectrophotometer (SUVS-M) of Central Aerological Observatory CAO, spectroradiometer of Meteorological Observatory of the Moscow State University (MO MSU) and model simulations based on delta-Eddington approximation

  15. Effects of UV-B radiation on leaf hair traits of invasive plants-Combining historical herbarium records with novel remote sensing data.

    Science.gov (United States)

    Václavík, Tomáš; Beckmann, Michael; Cord, Anna F; Bindewald, Anja M

    2017-01-01

    Ultraviolet-B (UV-B) radiation is a key but under-researched environmental factor that initiates diverse responses in plants, potentially affecting their distribution. To date, only a few macroecological studies have examined adaptations of plant species to different levels of UV-B. Here, we combined herbarium specimens of Hieracium pilosella L. and Echium vulgare L. with a novel UV-B dataset to examine differences in leaf hair traits between the plants' native and alien ranges. We analysed scans of 336 herbarium specimens using standardized measurements of leaf area, hair density (both species) and hair length (H. pilosella only). While accounting for other bioclimatic variables (i.e. temperature, precipitation) and effects of herbivory, we examined whether UV-B exposure explains the variability and geographical distribution of these traits in the native (Northern Hemisphere) vs. the alien (Southern Hemisphere) range. UV-B explained the largest proportion of the variability and geographical distribution of hair length in H. pilosella (relative influence 67.1%), and hair density in E. vulgare (66.2%). Corresponding with higher UV-B, foliar hairs were 25% longer for H. pilosella and 25% denser for E. vulgare in records from the Southern as compared to those from the Northern Hemisphere. However, focusing on each hemisphere separately or controlling for its effect in a regression analysis, we found no apparent influence of UV-B radiation on hair traits. Thus, our findings did not confirm previous experimental studies which suggested that foliar hairs may respond to higher UV-B intensities, presumably offering protection against detrimental levels of radiation. We cannot rule out UV-B radiation as a possible driver because UV-B radiation was the only considered variable that differed substantially between the hemispheres, while bioclimatic conditions (e.g. temperature, precipitation) and other considered variables (herbivory damage, collection date) were at similar

  16. Effects of UV-B radiation on leaf hair traits of invasive plants—Combining historical herbarium records with novel remote sensing data

    Science.gov (United States)

    Cord, Anna F.; Bindewald, Anja M.

    2017-01-01

    Ultraviolet-B (UV-B) radiation is a key but under-researched environmental factor that initiates diverse responses in plants, potentially affecting their distribution. To date, only a few macroecological studies have examined adaptations of plant species to different levels of UV-B. Here, we combined herbarium specimens of Hieracium pilosella L. and Echium vulgare L. with a novel UV-B dataset to examine differences in leaf hair traits between the plants’ native and alien ranges. We analysed scans of 336 herbarium specimens using standardized measurements of leaf area, hair density (both species) and hair length (H. pilosella only). While accounting for other bioclimatic variables (i.e. temperature, precipitation) and effects of herbivory, we examined whether UV-B exposure explains the variability and geographical distribution of these traits in the native (Northern Hemisphere) vs. the alien (Southern Hemisphere) range. UV-B explained the largest proportion of the variability and geographical distribution of hair length in H. pilosella (relative influence 67.1%), and hair density in E. vulgare (66.2%). Corresponding with higher UV-B, foliar hairs were 25% longer for H. pilosella and 25% denser for E. vulgare in records from the Southern as compared to those from the Northern Hemisphere. However, focusing on each hemisphere separately or controlling for its effect in a regression analysis, we found no apparent influence of UV-B radiation on hair traits. Thus, our findings did not confirm previous experimental studies which suggested that foliar hairs may respond to higher UV-B intensities, presumably offering protection against detrimental levels of radiation. We cannot rule out UV-B radiation as a possible driver because UV-B radiation was the only considered variable that differed substantially between the hemispheres, while bioclimatic conditions (e.g. temperature, precipitation) and other considered variables (herbivory damage, collection date) were at similar

  17. Effects of UV-B radiation on leaf hair traits of invasive plants-Combining historical herbarium records with novel remote sensing data.

    Directory of Open Access Journals (Sweden)

    Tomáš Václavík

    Full Text Available Ultraviolet-B (UV-B radiation is a key but under-researched environmental factor that initiates diverse responses in plants, potentially affecting their distribution. To date, only a few macroecological studies have examined adaptations of plant species to different levels of UV-B. Here, we combined herbarium specimens of Hieracium pilosella L. and Echium vulgare L. with a novel UV-B dataset to examine differences in leaf hair traits between the plants' native and alien ranges. We analysed scans of 336 herbarium specimens using standardized measurements of leaf area, hair density (both species and hair length (H. pilosella only. While accounting for other bioclimatic variables (i.e. temperature, precipitation and effects of herbivory, we examined whether UV-B exposure explains the variability and geographical distribution of these traits in the native (Northern Hemisphere vs. the alien (Southern Hemisphere range. UV-B explained the largest proportion of the variability and geographical distribution of hair length in H. pilosella (relative influence 67.1%, and hair density in E. vulgare (66.2%. Corresponding with higher UV-B, foliar hairs were 25% longer for H. pilosella and 25% denser for E. vulgare in records from the Southern as compared to those from the Northern Hemisphere. However, focusing on each hemisphere separately or controlling for its effect in a regression analysis, we found no apparent influence of UV-B radiation on hair traits. Thus, our findings did not confirm previous experimental studies which suggested that foliar hairs may respond to higher UV-B intensities, presumably offering protection against detrimental levels of radiation. We cannot rule out UV-B radiation as a possible driver because UV-B radiation was the only considered variable that differed substantially between the hemispheres, while bioclimatic conditions (e.g. temperature, precipitation and other considered variables (herbivory damage, collection date were at

  18. Increased resistance of environmental anaerobic spores to inactivation by UV

    NARCIS (Netherlands)

    Hijnen, W.A.M.; Veer, A.J. van der; Beerendonk, E.F.; Medema, Gerriet Jan

    2004-01-01

    Water Company Europoort started a pilot plant (MP)UV study to determine the UV-fluence to meet the Dutch drinking water standards. The results of large volume sampling of this pilot plant demonstrated that environmental spores of sulphite-reducing clostridia (SSRC) were highly resistant against UV.

  19. Exposure of Finnish population to solar UV radiation and consequent carcinogenic effects

    Energy Technology Data Exchange (ETDEWEB)

    Huurto, L; Jansen, C [Turku Univ. Hospital, Turku (Finland); Jokela, K [Finnish Centre for Radiation and Nuclear Safety, Helsinki (Finland)

    1997-12-31

    Depletion of stratospheric ozone increases irradiance of terrestrial ultraviolet (UV) radiation at short wavelengths, which may be harmful to the human health. To understand quantitatively the risks caused by increasing UV radiation to the Finnish population, the actual UV exposure of the population has to be assessed. It was shown that the snow reflection increases the UV exposure to the face and eyes particularly in the northern Finland. In 1993 exceptionally low ozone levels persisted up to the end of May, which resulted in a theoretical increase in the annual UV dose ranging from 8 % to 13 % in Finland. The maximal increase in the measured erythemally effective dose rate was 34 % on 23 April, when compared with the theoretical normal value. During this study exposure models have been developed. The models have been combined them with Green`s radiation transfer model to estimate annual facial UV doses received by different groups of Finnish population. Also, an updated estimate for increase in skin cancer incidence due to the ozone depletion is presented. It is estimated that the maximal increase in UV doses caused by the depletion of the stratospheric ozone will be 12 % in the first years of the next century in Finland. This may result in increase in skin carcinomas by 20-30 % if the people do not improve their protection against solar UV radiation. At the moment the annual facial UV dose of the Finnish indoor worker varies from 3 % to 6 % of the annual ambient dose. In the worst case an outdoor worker may receive even 16% of the annual ambient dose. However, the doses received by indoor workers during vacation to an untanned skin may be more harmful due to the increased risk of malignant melanoma.

  20. Exposure of Finnish population to solar UV radiation and consequent carcinogenic effects

    Energy Technology Data Exchange (ETDEWEB)

    Huurto, L.; Jansen, C. [Turku Univ. Hospital, Turku (Finland); Jokela, K. [Finnish Centre for Radiation and Nuclear Safety, Helsinki (Finland)

    1996-12-31

    Depletion of stratospheric ozone increases irradiance of terrestrial ultraviolet (UV) radiation at short wavelengths, which may be harmful to the human health. To understand quantitatively the risks caused by increasing UV radiation to the Finnish population, the actual UV exposure of the population has to be assessed. It was shown that the snow reflection increases the UV exposure to the face and eyes particularly in the northern Finland. In 1993 exceptionally low ozone levels persisted up to the end of May, which resulted in a theoretical increase in the annual UV dose ranging from 8 % to 13 % in Finland. The maximal increase in the measured erythemally effective dose rate was 34 % on 23 April, when compared with the theoretical normal value. During this study exposure models have been developed. The models have been combined them with Green`s radiation transfer model to estimate annual facial UV doses received by different groups of Finnish population. Also, an updated estimate for increase in skin cancer incidence due to the ozone depletion is presented. It is estimated that the maximal increase in UV doses caused by the depletion of the stratospheric ozone will be 12 % in the first years of the next century in Finland. This may result in increase in skin carcinomas by 20-30 % if the people do not improve their protection against solar UV radiation. At the moment the annual facial UV dose of the Finnish indoor worker varies from 3 % to 6 % of the annual ambient dose. In the worst case an outdoor worker may receive even 16% of the annual ambient dose. However, the doses received by indoor workers during vacation to an untanned skin may be more harmful due to the increased risk of malignant melanoma.

  1. Improving radiation data quality of USDA UV-B monitoring and research program and evaluating UV decomposition in DayCent and its ecological impacts

    Science.gov (United States)

    Chen, Maosi

    Solar radiation impacts many aspects of the Earth's atmosphere and biosphere. The total solar radiation impacts the atmospheric temperature profile and the Earth's surface radiative energy budget. The solar visible (VIS) radiation is the energy source of photosynthesis. The solar ultraviolet (UV) radiation impacts plant's physiology, microbial activities, and human and animal health. Recent studies found that solar UV significantly shifts the mass loss and nitrogen patterns of plant litter decomposition in semi-arid and arid ecosystems. The potential mechanisms include the production of labile materials from direct and indirect photolysis of complex organic matters, the facilitation of microbial decomposition with more labile materials, and the UV inhibition of microbes' population. However, the mechanisms behind UV decomposition and its ecological impacts are still uncertain. Accurate and reliable ground solar radiation measurements help us better retrieve the atmosphere composition, validate satellite radiation products, and simulate ecosystem processes. Incorporating the UV decomposition into the DayCent biogeochemical model helps to better understand long-term ecological impacts. Improving the accuracy of UV irradiance data is the goal of the first part of this research and examining the importance of UV radiation in the biogeochemical model DayCent is the goal of the second part of the work. Thus, although the dissertation is separated into two parts, accurate UV irradiance measurement links them in what follows. In part one of this work the accuracy and reliability of the current operational calibration method for the (UV-) Multi-Filter Rotating Shadowband Radiometer (MFRSR), which is used by the U.S. Department of Agriculture UV-B Monitoring and Research Program (UVMRP), is improved. The UVMRP has monitored solar radiation in the 14 narrowband UV and VIS spectral channels at 37 sites across U.S. since 1992. The improvements in the quality of the data result

  2. Exclusion of solar UV radiation improves photosynthetic performance and yield of wheat varieties.

    Science.gov (United States)

    Kataria, Sunita; Guruprasad, K N

    2015-12-01

    Field studies were conducted to determine the potential for alterations in photosynthetic performance and grain yield of four wheat (Triticum aestivum) varieties of India- Vidisha, Purna, Swarna and Naveen Chandausi by ambient ultraviolet radiation (UV). The plants were grown in specially designed UV exclusion chambers, wrapped with filters that excluded UV-B (solar UV exclusion increased the leaf mass per area ratio, leaf weight ratio and chlorophylls per unit area of flag leaves in all the four varieties of wheat. Polyphasic chlorophyll a fluorescence transients from the flag leaves of UV excluded wheat plants gave a higher fluorescence yield. Exclusion of solar UV significantly enhanced photosynthetic performance as a consequence of increased efficiency of PS II, performance index (PIABS) and rate of photosynthesis in the flag leaves of wheat varieties along with a remarkable increase in carbonic anhydrase, Rubisco and nitrate reductase activities. This additional fixation of carbon and nitrogen by exclusion of UV was channelized towards the improvement in grain yield of wheat varieties as there was a decrease in the UV-B absorbing substances and an increase in soluble protein content in flag leaves of all the four varieties of wheat. The magnitude of response for UV exclusion for all the measured parameters was higher in two varieties of wheat Vidisha and Purna as compared to Swarna and Naveen Chandausi. Cumulative stress response index (CSRI) for each variety was developed from the cumulative sum of physiological and yield parameters such as leaf mass area ratio of flag leaf, total chlorophyll content, performance index at absorption basis, rate of photosynthesis and grain yield. All the varieties had a negative CSRI, demonstrating a negative impact of ambient UV radiation. Naveen Chandausi and Swarna are less sensitive to ambient UV radiation; Vidisha is more sensitive to both UV-A and UV-B and Purna is more sensitive to ambient UV-B radiation. Copyright

  3. Absorption of uv-radiation by Chibro trademark -Uvelin eye drops

    International Nuclear Information System (INIS)

    Schreder, J.G.; Blumthaler, M.; Daxer, A.; Ettl, A.

    1998-01-01

    The preparation Chibro trademark -Uvelin is recommended against both solar and artificial uv-radiation. At wavelengths greater than 290 nm and at an effective layer thickness of 10 μm measurements with a high resolution doublemonochromator showed a transmission larger than 90%. The solar spectrum is characterised by almost no irradiance on the earth's surface for wavelengths smaller than 290 nm due to absorption in the atmospheric ozon layer. Therefore, no significant protective effect of the eye-drops against keratitis and cataract under solar exposure can be expected. A significantly better sagging of the radiation of artificial UV-sources with a high amount of UV-C is reached. The recommended application as chemical protective goggles in this spectral range is not permissible. (orig.) [de

  4. The effect of UV-B radiation on chloroplast translation in Pisum sativum

    International Nuclear Information System (INIS)

    Raab, M.M.; Jagendorf, A.T.

    1990-01-01

    UV-B radiation has previously been reported to reduce growth, flowering, and net photosynthesis. The present study examines the effect of UV-B radiation on isolated chloroplast of 7-10 day old pea seedlings. Amount of ( 3 H)-Leu incorporated into isolated chloroplasts was measured in the presence or absence of UV-B exposure. Preliminary experiments show a 30% inhibition of protein synthesis in isolated chloroplasts after only 20 mins of UV-B exposure (6.9 J/m 2 /30 min). Percent inhibition of chloroplast translation is directly correlated with UV-B exposure over a 60 min time span. Preliminary studies also show no change in both cold and radiolabeled protein profiles as expressed on 1-D PAGE and autofluorography. Comparative studies on the sensitivity of e - flow vs protein synthesis following UV-B exposure are underway. Further work on the role of oxygen free radicals and the specific site of action of UV-B damage to the translation machinery of chloroplasts will be discussed

  5. The role of coccoliths in protecting Emiliania huxleyi against stressful light and UV radiation

    Science.gov (United States)

    Xu, Juntian; Bach, Lennart T.; Schulz, Kai G.; Zhao, Wenyan; Gao, Kunshan; Riebesell, Ulf

    2016-08-01

    Coccolithophores are a group of phytoplankton species which cover themselves with small scales (coccoliths) made of calcium carbonate (CaCO3). The reason why coccolithophores form these calcite platelets has been a matter of debate for decades but has remained elusive so far. One hypothesis is that they play a role in light or UV protection, especially in surface dwelling species like Emiliania huxleyi, which can tolerate exceptionally high levels of solar radiation. In this study, we tested this hypothesis by culturing a calcified and a naked strain under different light conditions with and without UV radiation. The coccoliths of E. huxleyi reduced the transmission of visible radiation (400-700 nm) by 7.5 %, that of UV-A (315-400 nm) by 14.1 % and that of UV-B (280-315 nm) by 18.4 %. Growth rates of the calcified strain (PML B92/11) were about 2 times higher than those of the naked strain (CCMP 2090) under indoor constant light levels in the absence of UV radiation. When exposed to outdoor conditions (fluctuating sunlight with UV radiation), growth rates of calcified cells were almost 3.5 times higher compared to naked cells. Furthermore, the relative electron transport rate was 114 % higher and non-photochemical quenching (NPQ) was 281 % higher in the calcified compared to the naked strain, implying higher energy transfer associated with higher NPQ in the presence of calcification. When exposed to natural solar radiation including UV radiation, the maximal quantum yield of photosystem II was only slightly reduced in the calcified strain but strongly reduced in the naked strain. Our results reveal an important role of coccoliths in mitigating light and UV stress in E. huxleyi.

  6. Effects of the ultraviolet-B radiation (UV-B) on conifers: a review

    International Nuclear Information System (INIS)

    Laakso, K.; Huttunen, S.

    1998-01-01

    The current knowledge on conifer responses to enhanced ultraviolet-B (UV-B) radiation is mainly based on greenhouse or growth chamber experiments of one growing season in duration. However, the biomass losses observed in greenhouses do not occur in field-grown trees in their natural habitats. Moreover, the majority of the 20 conifer species studied have been 1-year-old seedlings, and no studies have been undertaken on mature trees. Fully grown needles, with their glaucous waxy surfaces and thick epidermal cells with both soluble and wall-bound UV-B screening metabolites, are well protected against UV-B radiation. However, it is not known whether these are sufficient protectants in young emerging needles or during the early spring period of high UV-B levels reflected from snow. In order to understand all the mechanisms that result in the protection of conifer needles against UV-B radiation, future research should focus on the epidermal layer, separating the waxes, cuticle and epidermal and hypodermal cells. Parallel studies should consist of wall-bound and soluble secondary metabolite analysis, antioxidant measurements and microscopic observations. (author)

  7. Differential flavonoid response to enhanced UV-B radiation in Brassica napus

    International Nuclear Information System (INIS)

    Olsson, L.C.; Veit, M.; Weissenböck, G.; Bornman, J.F.

    1998-01-01

    We have examined the qualitative and quantitative differences in methanol-soluble flavonoids of leaves of two cultivars of Brassica napus, which were grown with or without (control) supplemental UV-B radiation. The flavonoids were identified using HPLC-diode array spectroscopy (-DAS), -electrospray ionization-mass spectroscopy (-ESI-MS) and 1H and 13C NMR, and quantitatively analysed by HPLC-DAS. After exposure to supplementary UV-B radiation, the overall amount of soluble flavonoids, kaempferol and quercetin glycosides, increased by ca 150% in cv. Paroll, compared to control plants. Cultivar Stallion showed a 70% increase, and also a lower overall content of soluble flavonoids compared to Paroll. The supplementary UV-B radiation resulted in a marked, specific increase in the amount of quercetin glycosides relative to the kaempferol glycosides with a 36- and 23-fold increase in cvs Paroll and Stallion, respectively. Four of the flavonol glycosides appearing after supplemental UV-B exposure were identified as quercetin- and kaempferol 3-sophoroside-7-glucoside and 3-(2″′-E-sinapoylsophoroside)-7-glucoside. (author)

  8. UV radiation hardness of silicon inversion layer solar cells

    International Nuclear Information System (INIS)

    Hezel, R.

    1990-01-01

    For full utilization of the high spectral response of inversion layer solar cells in the very-short-wavelength range of the solar spectrum sufficient ultraviolet-radiation hardness is required. In addition to the charge-induced passivation achieved by cesium incorporation into the silicon nitride AR coating, in this paper the following means for further drastic reduction of UV light-induced effects in inversion layer solar cells without encapsulation are introduced and interpretations are given: increasing the nitride deposition temperature, silicon surface oxidation at low temperatures, and texture etching and using higher substrate resistivities. High UV radiation tolerance and improvement of the cell efficiency could be obtained simultaneously

  9. Effects of UV-B radiation on growth, photosynthesis, UV-B-absorbing compounds and NADP-malic enzyme in bean (Phaseolus vulgaris L.) grown under different nitrogen conditions.

    Science.gov (United States)

    Pinto, M E; Casati, P; Hsu, T P; Ku, M S; Edwards, G E

    1999-02-01

    The effects of UV-B radiation on growth, photosynthesis, UV-B-absorbing compounds and NADP-malic enzyme have been examined in different cultivars of Phaseolous vulgaris L. grown under 1 and 12 mM nitrogen. Low nitrogen nutrition reduces chlorophyll and soluble protein contents in the leaves and thus the photosynthesis rate and dry-matter accumulation. Chlorophyll, soluble protein and Rubisco contents and photosynthesis rate are not significantly altered by ambient levels of UV-B radiation (17 microW m-2, 290-320 nm, 4 h/day for one week). Comparative studies show that under high nitrogen, UV-B radiation slightly enhances leaf expansion and dry-matter accumulation in cultivar Pinto, but inhibits these parameters in Vilmorin. These results suggest that the UV-B effect on growth is mediated through leaf expansion, which is particularly sensitive to UV-B, and that Pinto is more tolerant than Vilmorin. The effect of UV-B radiation on UV-B-absorbing compounds and on NADP-malic enzyme (NADP-ME) activity is also examined. Both UV-B radiation and low-nitrogen nutrition enhance the content of UV-B-absorbing compounds, and among the three cultivars used, Pinto exhibits the highest increases and Arroz the lowest. The same trend is observed for the specific activity and content of NADP-ME. On a leaf-area basis, the amount of UV-B-absorbing compounds is highly correlated with the enzyme activity (r2 = 0.83), suggesting that NADP-ME plays a key role in biosynthesis of these compounds. Furthermore, the higher sensitivity of Vilmorin than Pinto to UV-B radiation appears to be related to the activity of NADP-ME and the capacity of the plants to accumulate UV-B-absorbing compounds.

  10. Near-UV radiation acts as a beneficial factor for physiological responses in cucumber plants.

    Science.gov (United States)

    Mitani-Sano, Makiko; Tezuka, Takafumi

    2013-11-05

    Effects of near-UV radiation on the growth and physiological activity of cucumber plants were investigated morphologically, physiologically and biochemically using 3-week-old seedlings grown under polyvinyl chloride films featuring transmission either above 290 nm or above 400 nm in growth chambers. The hypocotyl length and leaf area of cucumber seedlings were reduced but the thickness of leaves was enhanced by near-UV radiation, due to increased upper/lower epidermis thickness, palisade parenchyma thickness and volume of palisade parenchyma cells. Photosynthetic and respiratory activities were also promoted by near-UV radiation, associated with general enhancement of physiological/biochemical responses. Particularly, metabolic activities in the photosynthetic system of chloroplasts and the respiratory system of mitochondria were analyzed under the conditions of visible light with and without near-UV radiation. For example, the activities of NAD(P)-dependent enzymes such as glyceraldehyde-3-phosphate dehydrogenase (G3PDH) in chloroplasts and isocitrate dehydrogenase (ICDH) in mitochondria were elevated, along with levels of pyridine nucleotides (nicotinamide coenzymes) [NAD(H) and NADP(H)] and activity of NAD kinase (NADP forming enzyme). Taken together, these data suggest that promotion of cucumber plant growth by near-UV radiation involves activation of carbon and nitrogen metabolism in plants. The findings of this research showed that near-UV radiation reaching the Earth's surface is a beneficial factor for plant growth. Copyright © 2013. Published by Elsevier B.V.

  11. The responses to supplementary of UV radiation of some temperate meadow species

    International Nuclear Information System (INIS)

    Cooley, N.M.

    2002-01-01

    Full text: The growth and development of various meadow species was monitored while growing under enhanced UV-radiation in the natural light environment. Growth responses to supplementary ultraviolet-B (UV-B+A) and ultraviolet-A (UV-A) were compared to the ambient daylight treatment for Bellis perennis, Cardamine pratensis, Cynosurus critatus and Ranunculus ficaria. When the response of ultraviolet A (UV-A) treated plants were compared with those of the UV-B+A, differences were found which varied according to the species and parameter investigated. To further understand the growth responses of the UV-A treatment and their relationship to the UV-B responses polychromatic action spectra in the natural environment was employed B perennis had an action maximum in the UV B (280-315 nm) while C cristatus demonstrates no action in the UV-B but action in the UV-A region (315-400 nm.). To enable further explanation of the effects of elevated UV radiation on the meadow plants Arabidopsis thaliana ecotypes and mutants were investigated. A thaliana ecotypes dry weight accumulation was found to respond differently to the UV treatments. UV B+A treatment was found to inhibit dry weight accumulation in most ecotypes. When UV B+A induced inhibition was expressed in terms of ambient growth rate for each ecotype a linear relationship could be derived. The higher the growth rate the more susceptible the ecotype was to UV-B+A inhibition. The pertinence of the UV-A treatment and UV protocol is discussed. It is suggested that UV responses could alter the diversity of the meadow equilibrium

  12. Effect of elevated CO2, O3, and UV radiation on soils.

    Science.gov (United States)

    Formánek, Pavel; Rejšek, Klement; Vranová, Valerie

    2014-01-01

    In this work, we have attempted to review the current knowledge on the impact of elevated CO2, O3, and UV on soils. Elevated CO2 increases labile and stabile soil C pool as well as efficiency of organic pollutants rhizoremediation and phytoextraction of heavy metals. Conversely, both elevated O3 and UV radiation decrease inputs of assimilates to the rhizosphere being accompanied by inhibitory effects on decomposition processes, rhizoremediation, and heavy metals phytoextraction efficiency. Contrary to elevated CO2, O3, or UV-B decreases soil microbial biomass, metabolisable C, and soil N t content leading to higher C/N of soil organic matter. Elevated UV-B radiation shifts soil microbial community and decreases populations of soil meso- and macrofauna via direct effect rather than by induced changes of litter quality and root exudation as in case of elevated CO2 or O3. CO2 enrichment or increased UV-B is hypothesised to stimulate or inhibit both plant and microbial competitiveness for soluble soil N, respectively, whereas O3 favours only microbial competitive efficiency. Understanding the consequences of elevated CO2, O3, and UV radiation for soils, especially those related to fertility, phytotoxins inputs, elements cycling, plant-microbe interactions, and decontamination of polluted sites, presents a knowledge gap for future research.

  13. The effect of UV-B and UV-C radiation on Hibiscus leaves determined by ultraweak luminescence and fluorescence induction [chlorophyll fluorescence induction, ultraweak luminescence

    International Nuclear Information System (INIS)

    Panagopoulos, I.; Bornman, J.F.; Björn, L.O.

    1989-01-01

    The effects of UV-C (254 nm) and UV-B (280-320 nm) on chlorophyll fluorescence induction and ultraweak luminescence (UL) in detached leaves of Hibiscus rosa-sinensis L. were investigated. UL from leaves exposed to UV-B and UV-C radiation reached a maximum 72 h after irradiation. In both cases most of the light was of a wavelength over 600 nm. An increase in the percentage of long wavelength light with time was detected. UV radiation increased peroxidase activity, which also reached a maximum 72 h after irradiation. UV-B and UV-C both reduced variable chlorophyll fluorescence. No effect on the amount of chlorophyll or UV screening pigments was observed with the short-term irradiation used in this investigation. (author)

  14. Adverse Effects of UV-B Radiation on Plants Growing at Schirmacher Oasis, East Antarctica.

    Science.gov (United States)

    Singh, Jaswant; Singh, Rudra P

    2014-01-01

    This study aimed to assess the impacts of ultraviolet-B (UV-B) radiation over a 28-day period on the levels of pigments of Umbilicaria aprina and Bryum argenteum growing in field. The depletion of stratospheric ozone is most prominent over Antarctica, which receives more UV-B radiation than most other parts of the planet. Although UV-B radiation adversely affects all flora, Antarctic plants are better equipped to survive the damaging effects of UV-B owing to defenses provided by UV-B absorbing compounds and other screening pigments. The UV-B radiations and daily average ozone values were measured by sun photometer and the photosynthetic pigments were analyzed by the standard spectrophotometric methods of exposed and unexposed selected plants. The daily average atmospheric ozone values were recorded from 5 January to 2 February 2008. The maximum daily average for ozone (310.7 Dobson Units (DU)) was recorded on 10 January 2008. On that day, average UV-B spectral irradiances were 0.016, 0.071, and 0.186 W m(-2) at wavelengths of 305, 312, and 320 nm, respectively. The minimum daily average ozone value (278.6 DU) was recorded on 31 January 2008. On that day, average UV-B spectral irradiances were 0.018, 0.085, and 0.210 W m(-2) at wavelengths of 305, 312, and 320 nm, respectively. Our results concludes that following prolonged UV-B exposure, total chlorophyll levels decreased gradually in both species, whereas levels of UV-B absorbing compounds, phenolics, and carotenoids gradually increased.

  15. The response of bean plants to UV-B radiation under different irradiances of background visible light

    International Nuclear Information System (INIS)

    Cen, Y.P.; Bornman, J.F.

    1990-01-01

    Plants of Phaseolus vulgaris L. (cv. Stella) were grown in controlled conditions under three different irradiances of visible light with or without UV-B (280–320nm) radiation. The biologically effective UV-B radiation (UV-BBE) was 6.17 kJ m −2 d −1 , and simulated a c. 5% decrease in stratospheric ozone at 55.7°N, 13.4°E. The photon flux densities of the photosynthetically active radiation (PAR, 400–700 nm) were either 700 μmol m −2−1 (HL), 500, μmol m −2 s −1 (ML) or 230 μmol m −2 s −1 PAR (LL). Under high light (HL) conditions plus UV-B radiation, bean plants appeared most resistant to the enhanced levels of UV-B radiation, and responded only by increasing leaf thickness by c. 18%. A small increase in UV screening pigments was also observed. Both the lower irradiances (ML and LL) increased the sensitivity of the plants to UV-B radiation. Changes in leaf structure were also observed. Photosystem II was inhibited under ML and LL together with UV-B radiation, as determined by Chi fluorescence induction and calculation of the fluorescence half-rise times. Leaf reflectivity measurements showed that the amount of PAR able to penetrate leaves of UV-B treated plants was reduced, and that a possible correlation may exist between the reduced PAR levels, loss of Chi and lowered photosynthetic activity, especially for LL +UV-B grown plants, where surface reflection from leaves was highest. Changes in leaf chlorophyll content were mostly confined to plants grown under LL + UV-B, where a decrease of c. 20% was found. With regard to protective pigments (the carotenoids and UV screening pigments) plants subjected to different visible light conditions responded differently. Among the growth parameters measured, there was a substantial decrease in leaf area, particularly under LL + UV-B (c. 47% relative to controls), where leaf dry weight was also reduced by c. 25%. (author)

  16. UV Deprivation Influences Social UV Preference in Juvenile Sticklebacks

    Directory of Open Access Journals (Sweden)

    Ricarda Modarressie

    2015-05-01

    Full Text Available Social aggregations occur in many different animal taxa and mainly result from non-random assortment. Investigating factors that shape and maintain the composition of social aggregations are among others a main topic for understanding ecological speciation processes. Aggregation decisions are mediated by olfactory and visual cues, which in many animals are extended into the UV part of the electromagnetic spectrum. Here, we were interested in developmental plasticity of social preferences with respect to UV radiation in aquatic organisms. Specifically, we tested whether different lighting environments with respect to UV wavelengths during early life stages influence the shoaling preference in juvenile threespine sticklebacks (Gasterosteus aculeatus. Family (full-sibling groups were split and reared under UV-lacking (UV- and UV-present (UV+ lighting conditions. Subsequent shoal choice experiments, in which test fish from both rearing conditions could simultaneously choose between a shoal seen behind a UV-blocking (UV- and a shoal seen behind a UV-transmitting (UV+ filter, revealed a significant effect of lighting condition during rearing on association preference. Test fish that had been deprived of UV spent significantly more time near the UV- shoal compared to the test fish reared under full-spectrum lighting conditions. The results are discussed with respect to plasticity of the visual system and environmental lighting conditions.

  17. Investigations of the effects of UV and X-ray radiation and the repair of radiation damage in the ciliate Stylonychia mytilus

    International Nuclear Information System (INIS)

    Dittmann, F.N.

    1978-01-01

    Using the example of Stylomychia mytilus, the effects of UV-radiation and ionizing X-ray radiation are compared. The effects on cell division and on the repair of radiation damage in DNA are compared. Sensitivity to UV radiation differs between the stages of the cell cycle while the effects of X-ray radiation are independent of phase. There is no difference in repair processes. (AJ) 891 AJ/AJ 892 MKO [de

  18. Effects of UV-B radiation on the isoflavone accumulation and physiological-biochemical changes of soybean during germination: Physiological-biochemical change of germinated soybean induced by UV-B.

    Science.gov (United States)

    Ma, Meng; Wang, Pei; Yang, Runqiang; Gu, Zhenxin

    2018-06-01

    In this study, the effects of UV-B radiation on the isoflavones accumulation, physiological and nutritional quality, water status, and characteristics of proteins in germinated soybeans were investigated. The results showed that isoflavones content in soybeans increased with appropriate intensity and time of UV-B radiation and decreased with excessive treatment. Fresh weight, length, free amino acids, reducing sugar contents and bulk water (T 23 ) in germinated soybeans decreased with increasing radiation time, indicating that UV-B inhibited the growth and nutrients metabolism of soybean during germination. Cell damage was detected in germinated soybeans with excessive UV-B radiation, as shown by the black spots in cotyledons and the increased intercellular water determined by LF-NMR. Germination resulted in an increase in random coil structures, while UV-B radiation induced no obvious changes in FT-IR spectrum and protein conformation of soybeans. Both UV-B radiation and germination caused the increase in soluble proteins, especially in 1.0-75.0 kDa fraction. Copyright © 2018 Elsevier Ltd. All rights reserved.

  19. UV-C radiation based methods for aqueous metoprolol elimination

    Energy Technology Data Exchange (ETDEWEB)

    Rivas, F.J., E-mail: fjrivas@unex.es [Departamento de Ingenieria Quimica y Quimica Fisica, Universidad de Extremadura, Facultad de Ciencias, Edificio Jose Luis Sotelo, Avenida de Elvas S/N, 06071 Badajoz (Spain); Gimeno, O.; Borralho, T.; Carbajo, M. [Departamento de Ingenieria Quimica y Quimica Fisica, Universidad de Extremadura, Facultad de Ciencias, Edificio Jose Luis Sotelo, Avenida de Elvas S/N, 06071 Badajoz (Spain)

    2010-07-15

    The endocrine disruptor metoprolol has been oxidised in aqueous solution by means of the systems UV-C, UV-C/H{sub 2}O{sub 2}, UV-C/percarbonate, UV-C/monopersulfate, UV-C/TiO{sub 2}, UV-C/H{sub 2}O{sub 2}/TiO{sub 2} and photo-Fenton. From simple photolysis experiments the quantum yield of metoprolol has been calculated (roughly 5 x 10{sup -3} mol Einstein{sup -1} at circumneutral pH). Addition of free radicals promoters significantly enhanced the metoprolol depletion rate. Mineralization degree was negligible when no promoter was added, while low values were achieved in the presence of either inorganic peroxides or titanium dioxide. The combination of radiation, hydrogen peroxide and TiO{sub 2} increased the mineralization level up to values in the proximity of 45-50% under the best conditions investigated. The photo-Fenton process was the best system in terms of total oxidation (mineralization degree 70%) when optimum conditions were applied.

  20. UV-C radiation based methods for aqueous metoprolol elimination

    International Nuclear Information System (INIS)

    Rivas, F.J.; Gimeno, O.; Borralho, T.; Carbajo, M.

    2010-01-01

    The endocrine disruptor metoprolol has been oxidised in aqueous solution by means of the systems UV-C, UV-C/H 2 O 2 , UV-C/percarbonate, UV-C/monopersulfate, UV-C/TiO 2 , UV-C/H 2 O 2 /TiO 2 and photo-Fenton. From simple photolysis experiments the quantum yield of metoprolol has been calculated (roughly 5 x 10 -3 mol Einstein -1 at circumneutral pH). Addition of free radicals promoters significantly enhanced the metoprolol depletion rate. Mineralization degree was negligible when no promoter was added, while low values were achieved in the presence of either inorganic peroxides or titanium dioxide. The combination of radiation, hydrogen peroxide and TiO 2 increased the mineralization level up to values in the proximity of 45-50% under the best conditions investigated. The photo-Fenton process was the best system in terms of total oxidation (mineralization degree 70%) when optimum conditions were applied.

  1. Mean Annual UV-B Irradiance

    Data.gov (United States)

    U.S. Environmental Protection Agency — Ultraviolet-B (UV-B) radiation is the most energetic part of sunlight reaching the Earth's surface (wavelength region is 280 to 315 nm), and it has been shown to...

  2. DNA repair pathways involved in determining the level of cytotoxicity of environmentally relevant UV radiation

    International Nuclear Information System (INIS)

    Carpenter, L.

    2000-01-01

    The sensitivity of cell lines with defects in various DNA repair processes to different wavelengths of UV has been assessed in order to determine the importance of these repair pathways to the cytotoxicity of UV light. The cell lines used in this work were xrs-6 (a Chinese Hamster Ovary (CHO) cell line) mutant for XRCC5/Ku80, EM9 a CHO cell line mutant for XRCC1, UV61 a CHO cell line mutant for ERCC6/CSB, and E3p53-/-, a mouse embryonic fibroblast cell line null for p53. Xrs-6 (defective in Non Homologous End-Joining) was found to be sensitive to the cytotoxic effects of broadband UVA, but not narrowband UVA or narrowband UVB. EM9 (defective in Base Excision Repair/Single-Strand Break Repair) was not sensitive to the cytotoxic effects of both broadband and narrowband UVA, narrowband UVB or narrowband UVC. UV61 (defective in the Transcription Coupled Repair branch of Nucleotide Excision Repair) was sensitive to the cytotoxic effects of narrowband UVA, UVB and UVC. E3p53-/- was sensitive to the cytotoxic effects of narrowband UVA and UVB. Broadband UVA was found to induce high levels of chromosomal damage in xrs-6, as quantified by the micronucleus assay, most likely as a result of this cell lines inability to repair DNA double strand breaks. EM9 was found to be defective in the repair of broadband UVA-induced single strand breaks, as measured by the alkaline gel electrophoresis ('comet') assay. UV61 was unable to repair broadband UVB-induced DNA damage as measured by the alkaline gel electrophoresis ('comet') assay. These results provide evidence that: 1. DNA double-strand breaks contribute to the cytotoxicity of UVA to a greater extent than single-strand breaks. 2. Repair mechanisms that operate in response to UVA may be coupled to transcription. 3. UVB may directly induce SSBs. 4. P53 is involved in the response of the cell to both UVA and UVB radiation. (author)

  3. Influence of low ozone episodes on erythemal UV-B radiation in Austria

    Science.gov (United States)

    Schwarz, Matthias; Baumgartner, Dietmar J.; Pietsch, Helga; Blumthaler, Mario; Weihs, Philipp; Rieder, Harald E.

    2017-06-01

    This study investigates the influence of low ozone episodes on UV-B radiation in Austria during the period 1999 to 2015. To this aim observations of total column ozone (TCO) in the Greater Alpine Region (Arosa, Switzerland; Hohenpeissenberg, Germany; Hradec Kralove, Czech Republic; Sonnblick, Austria), and erythemal UV-B radiation, available from 12 sites of the Austrian UV-B monitoring network, are analyzed. As previous definitions for low ozone episodes are not particularly suited to investigate effects on UV radiation, a novel threshold approach—considering anomalies—is developed to provide a joint framework for the analysis of extremes. TCO and UV extremes are negatively correlated, although modulating effects of sunshine duration impact the robustness of the statistical relationship. Therefore, information on relative sunshine duration (SDrel), available at (or nearby) UV-B monitoring sites, is included as explanatory variable in the analysis. The joint analysis of anomalies of both UV index (UVI) and total ozone (∆UVI, ∆TCO) and SDrel across sites shows that more than 65% of observations with strongly negative ozone anomalies (∆TCO 1), we find (across all sites) that about 90% correspond to negative ∆TCO. The remaining 10% of days occurred during fair weather conditions (SDrel ≥ 80%) explaining the appearance of ∆UVI > 1 despite positive TCO anomalies. Further, we introduce an anomaly amplification factor (AAF), which quantifies the expected change of the ∆UVI for a given change in ∆TCO.

  4. UV radiation hazards

    International Nuclear Information System (INIS)

    Henderson, A.R.

    1987-01-01

    Exposure to ultraviolet radiation (UVR) is, for most people, a daily occurrence. Significant quantities of ultraviolet are present in sunlight, and this environmental exposure usually greatly exceeds that necessary for vitamin D production, the only certain benefit of UVR. In addition, occupational exposure to artificial sources of UVR is commonly encountered in commerce, industry and medicine. Exposure to UVR can present a hazard, principally to the eyes and exposed areas of the skin. The potential for any given source of UVR to cause photobiological damage depends on the spectral composition of the incident radiation, the geometry of optical coupling into the tissues at risk, the spectral sensitivity to damage of the irradiated tissue, the total accumulated exposure, and the action of any biological repair processes. In the ultraviolet region the photobiological interactions of concern are mainly photochemical. Hazard analysis and radiation protection require an appropriate framework of radiation measurement for the quantitative assessment of exposure and for the specification of safe exposure limits

  5. Effect of enhanced UV-B radiation on yield and quality of rice

    International Nuclear Information System (INIS)

    Yin Hong; Guo Wei; Mao Xiaoyan

    2009-01-01

    The effects of enhanced UV-B radiation on yield and quality of two rice cuhivars(ShenNong 6014 and ShenNong 265) are studied in potted method. There were three treatments including natural light (TCK), enhanced 5% UV-B radiation (T) and enhanced 10% (T). The results showed that enhanced UV-B radiation decreases yield components, the percentage of brown rice (0.66%-7.06%), head rice rate (5.65%-18.88%), the rate of white rice (22.17%-40.16%), grain area (2.61%-6.25%), fatty acid contents (1.23%-54.19%) and eating quality (1.07%-16.78%) but increasea protein content (4.65%-10.71%) and amylose content of rice (0.56%-4.81%). The effects of T2 was stronger than T1

  6. The environmental effects of radiation on flight crews

    International Nuclear Information System (INIS)

    Connor, C.W.

    1991-01-01

    A review is presented of a continuing investigation of flight deck radiation and its potential effects on flight crews. Attention is given to the various critical factors concerned in UV radiation exposure and detection including skin cancer classifications, skin types, effectiveness of different sun protection factors, and flight deck color configuration and sunglasses. Consideration is given to both UV and ionizing radiation

  7. Effects of Solar UV Radiation and Climate Change on Biogeochemical Cycling: Interactions and Feedbacks

    Science.gov (United States)

    Solar UV radiation, climate and other drivers of global change are undergoing significant changes and models forecast that these changes will continue for the remainder of this century. Here we assess the effects of solar UV radiation on biogeochemical cycles and the interactions...

  8. Effect of Elevated CO2, O3, and UV Radiation on Soils

    Directory of Open Access Journals (Sweden)

    Pavel Formánek

    2014-01-01

    Full Text Available In this work, we have attempted to review the current knowledge on the impact of elevated CO2, O3, and UV on soils. Elevated CO2 increases labile and stabile soil C pool as well as efficiency of organic pollutants rhizoremediation and phytoextraction of heavy metals. Conversely, both elevated O3 and UV radiation decrease inputs of assimilates to the rhizosphere being accompanied by inhibitory effects on decomposition processes, rhizoremediation, and heavy metals phytoextraction efficiency. Contrary to elevated CO2, O3, or UV-B decreases soil microbial biomass, metabolisable C, and soil Nt content leading to higher C/N of soil organic matter. Elevated UV-B radiation shifts soil microbial community and decreases populations of soil meso- and macrofauna via direct effect rather than by induced changes of litter quality and root exudation as in case of elevated CO2 or O3. CO2 enrichment or increased UV-B is hypothesised to stimulate or inhibit both plant and microbial competitiveness for soluble soil N, respectively, whereas O3 favours only microbial competitive efficiency. Understanding the consequences of elevated CO2, O3, and UV radiation for soils, especially those related to fertility, phytotoxins inputs, elements cycling, plant-microbe interactions, and decontamination of polluted sites, presents a knowledge gap for future research.

  9. Effect of Elevated CO2, O3, and UV Radiation on Soils

    Science.gov (United States)

    Rejšek, Klement; Vranová, Valerie

    2014-01-01

    In this work, we have attempted to review the current knowledge on the impact of elevated CO2, O3, and UV on soils. Elevated CO2 increases labile and stabile soil C pool as well as efficiency of organic pollutants rhizoremediation and phytoextraction of heavy metals. Conversely, both elevated O3 and UV radiation decrease inputs of assimilates to the rhizosphere being accompanied by inhibitory effects on decomposition processes, rhizoremediation, and heavy metals phytoextraction efficiency. Contrary to elevated CO2, O3, or UV-B decreases soil microbial biomass, metabolisable C, and soil Nt content leading to higher C/N of soil organic matter. Elevated UV-B radiation shifts soil microbial community and decreases populations of soil meso- and macrofauna via direct effect rather than by induced changes of litter quality and root exudation as in case of elevated CO2 or O3. CO2 enrichment or increased UV-B is hypothesised to stimulate or inhibit both plant and microbial competitiveness for soluble soil N, respectively, whereas O3 favours only microbial competitive efficiency. Understanding the consequences of elevated CO2, O3, and UV radiation for soils, especially those related to fertility, phytotoxins inputs, elements cycling, plant-microbe interactions, and decontamination of polluted sites, presents a knowledge gap for future research. PMID:24688424

  10. UV- and gamma-radiation sensitive mutants of Arabidopsis thaliana

    International Nuclear Information System (INIS)

    Jiang, C.Z.; Yen, C.N.; Cronin, K.; Mitchell, D.; Britt, A.B.

    1997-01-01

    Arabidopsis seedlings repair UV-induced DNA damage via light-dependent and -independent pathways. The mechanism of the ''dark repair'' pathway is still unknown. To determine the number of genes required for dark repair and to investigate the substrate-specificity of this process we isolated mutants with enhanced sensitivity to UV radiation in the absence of photoreactivating light. Seven independently derived UV sensitive mutants were isolated from an EMS-mutagenized population. These fell into six complementation groups, two of which (UVR1 and UVH1) have previously been defined. Four of these mutants are defective in the dark repair of UV-induced pyrimidine [6-4] pyrimidinone dimers. These four mutant lines are sensitive to the growth-inhibitory effects of gamma radiation, suggesting that this repair pathway is also involved in the repair of some type of gamma-induced DNA damage product. The requirement for the coordinate action of several different gene products for effective repair of pyrimidine dimers, as well as the nonspecific nature of the repair activity, is consistent with nucleotide excision repair mechanisms previously described in Saccharomyces cerevisiae and nonplant higher eukaryotes and inconsistent with substrate-specific base excision repair mechanisms found in some bacteria, bacteriophage, and fungi. (author)

  11. The fascinating diatom frustule—can it play a role for attenuation of UV radiation?

    DEFF Research Database (Denmark)

    Ellegaard, Marianne; Lenau, Torben Anker; Lundholm, Nina

    2016-01-01

    size range as wave lengths of visible and ultraviolet (UV) light. This has prompted research into the possible role of the frustule in mediating light for the diatoms’ photosynthesis as well as into possible photonic applications of the diatom frustule. One of the possible biological roles, as well...... as area of potential application, is UV protection. In this review, we explore the possible adaptive value of the silica frustule with focus on research on the effect of UV radiation ondiatoms. We also explore the possible effect of the frustules on UV radiation, from a theoretical, biological......, and applied perspective, including recent experimental data on UV transmission of diatom frustules....

  12. Effect of UV-B radiation on the marine diatom bellerochea yucatanensis

    International Nuclear Information System (INIS)

    Doehler, G.

    1982-01-01

    There exists no information about the UV-B fluence on several photosynthetic products and nitrogen metabolism. The present report describes the effect of low levels of UV-B radiation on pigments, 14 C- and 15 N-incorporation of the marine diatom Bellerochea yucatanensis. (orig./AJ)

  13. The sensitivity of sunflower (Helianthus annuus L. plants to UV-B radiation is altered by nitrogen status

    Directory of Open Access Journals (Sweden)

    Inês Cechin

    2018-02-01

    Full Text Available ABSTRACT: Interaction effects between nitrogen and UV-B radiation were studied in sunflower (Helianthus annuus L. variety IAC-Iarama plants grown in a greenhouse under natural photoperiod conditions. Plants were irradiated with 0.8W m-2 (control or 8.0W m-2 (+UV-B of UV-B radiation for 7h per day. The plants were grown in pots containing vermiculite and watered with 70% of full strength nitrogen-free Long Ashton solution, containing either low (42.3ppm or high (282ppm nitrogen as ammonium nitrate. High nitrogen increased dry matter of stem, leaves and shoot, photosynthetic pigments and photosynthesis (A without any alteration in stomatal conductance (gs nor transpiration (E while it reduced the intercellular CO2 (Ci concentration, and malondialdehyde (MDA content. High UV-B radiation had negative effects on dry matter production, A, gs and E with the effects more marked under high nitrogen, whereas it increased Ci under high nitrogen. Activity of PG-POD was reduced by high UV-B radiation under low nitrogen but it was not changed under high nitrogen. The UV-B radiation increased the MDA content independently of nitrogen level. Results indicate that the effects of UV-B radiation on sunflower plants are dependent of nitrogen supply with high nitrogen making their physiological processes more sensitive to UV-B radiation.

  14. Diurnal changes in CN metabolism and response of rice seedlings to UV-B radiation.

    Science.gov (United States)

    Yun, Hyejin; Lim, Sunhyung; Kim, Yangmin X; Lee, Yejin; Lee, Seulbi; Lee, Deogbae; Park, Keewoong; Sung, Jwakyung

    2018-03-13

    Plants regulate a number of primary metabolites, including carbohydrates, organic acids, and amino acids, in response to UV-B radiation. Therefore, it is essential to understand the time-dependent response of rice plants to UV-B stress. This study focused on the response of plants to UV-B at different leaf developmental phases (emerging, growing, and maturing) in an attempt to fully comprehend the metabolic shift. We analyzed the expression levels of genes related to starch/sucrose metabolism in the leaf blades of rice seedlings (Oryza sativa L. "Saechuchenog") exposed to UV-B irradiation for short (1 day) and long terms (5 days) using quantitative real-time polymerase chain reaction. We also examined the diurnal variations in the contents of primary metabolites using an established GCTOF-MS (gas chromatography time of flight-mass spectrometry) method. The results showed that the levels of primary metabolites were largely dependent upon the diurnal rhythm and leaf developmental phase. The young leaves (sink) produced and accumulated starch rather than sucrose. The short-term (4 h, 1 day) UV-B exposure inhibited sucrose synthesis, which could be the first target of UV-B radiation. Following short- and long-term (5 days) exposure to UV-B radiation, the dynamic response of primary metabolites was evaluated. It was found that the content of carbohydrates decreased throughout the period of exposure to UV-B stress, especially in terms of sucrose concentration. However, the content of the majority of amino acids increased after an early decrease. Our data revealed that the metabolic response, as well as the gene expression, differed with the period (intensity) of exposure to UV-B radiation and with the phase of leaf development. These findings provide new insights for a better understanding of the metabolic response of a variety of plant species exposed to a wide range of UV-B radiation. Copyright © 2018. Published by Elsevier GmbH.

  15. Electrophysiological and growing aspects of ultraviolet (UV-C) radiation action

    International Nuclear Information System (INIS)

    Karcz, W.

    1992-01-01

    Effects of UV-C (254 nm) radiation on electrical parameters and growth processes in plant cells were studied. It was found in Nitellopsis obtusa cells the UV-C radiation caused transient depolarization of plasmalemma and tonoplast and simultaneous increase in electric conductance. These effects were partly reversible and the degree of the recovery depended on the duration of the exposure, temperature of the medium and area of the irradiation. Exposure of Nitellopsis obtusa cells with large potential difference between vacuole and external medium (more negative than - 140 mV) brought about the generation of an action potential, whose shape depended on the duration of irradiation. In the cells pretreated with IAA in the dark or exposed to visible light, the UV-C irradiation not only abolished the hyper polarization induced by IAA or visible light, but caused a further depolarization. Similar effects of IAA and UV-C on membrane potential were demonstrated in cells of Zea mays L. coleoptile segments. The hyper polarized state created by visible light in Sagittaria leaf cells was also fully suppressed by the radiation. The growth experiments were based on elongation growth of Zea mays L. coleoptile segments and simultaneously measured changes of pH of the incubation medium. It was shown that for high doses of irradiation (1170, 3900, and 5850 J m -2 ) UV-C inhibited elongation growth, whereas at 195 J m -2 stimulation of growth was observed. The administration of IAA (10 -5 M) and FC (10 -6 M) to the incubation medium of coleoptile segments partially abolished the inhibitory effect of UV-C. The pH of the incubation medium showed that the exposure of the segments to UV-C caused inhibition H + -extrusion (or stimulation of H + uptake). The presence of IAA (10 -5 M) or FC (10 -6 M) in the incubation medium of irradiated coleoptile segments promoted H + -extrusion to a level comparable with that produced by IAA or FC in non-irradiated coleoptile segments. It is suggested

  16. Susceptibility of Campylobacter jejuni and Yersinia enterocolitica to UV radiation

    Energy Technology Data Exchange (ETDEWEB)

    Butler, R.C.; Lund, V.; Carlson, D.A.

    1987-02-01

    Two enteric pathogens, Campylobacter jejuni and Yersinia enterocolitica serogroup O:3, together with Escherichia coli, were investigated for susceptibility to UV radiation at 254 nm. The UV dose required for a 3-log reduction (99.9% inactivation) of C. jejuni, Y. enterocolitica, and E. coli was 1.8, 2.7, and 5.0 mWs/cm2, respectively. Using E. coli as the basis for comparison, it appears that C. jejuni and Y. enterocolitica serogroup O:3 are more sensitive to UV than many of the pathogens associated with waterborne disease outbreaks and can be easily inactivated in most commercially available UV reactors. No association was found between the sensitivity of Y. enterocolitica to UV and the presence of a 40- to 50-megadalton virulence plasmid.

  17. Susceptibility of Campylobacter jejuni and Yersinia enterocolitica to UV radiation

    International Nuclear Information System (INIS)

    Butler, R.C.; Lund, V.; Carlson, D.A.

    1987-01-01

    Two enteric pathogens, Campylobacter jejuni and Yersinia enterocolitica serogroup O:3, together with Escherichia coli, were investigated for susceptibility to UV radiation at 254 nm. The UV dose required for a 3-log reduction (99.9% inactivation) of C. jejuni, Y. enterocolitica, and E. coli was 1.8, 2.7, and 5.0 mWs/cm2, respectively. Using E. coli as the basis for comparison, it appears that C. jejuni and Y. enterocolitica serogroup O:3 are more sensitive to UV than many of the pathogens associated with waterborne disease outbreaks and can be easily inactivated in most commercially available UV reactors. No association was found between the sensitivity of Y. enterocolitica to UV and the presence of a 40- to 50-megadalton virulence plasmid

  18. Changes in epicuticular flavonoids and photosynthetic pigments as a plant response to UV-B radiation

    Energy Technology Data Exchange (ETDEWEB)

    Cuadra, P.; Harborne, J. B. [Universidad de Magallances, Punta Arenas (Chile)

    1996-07-01

    Treatment of Gnaphalium vira-vira plants with UV-B radiation caused changes in plant growth and in plant chemistry. The leaf surface contained two O-methylated flavones, araneol and 7-O-methylaraneol. HPLC analysis showed that 20 days of UV-B radiation increased the synthesis of 7-O-methylaraneol at the expense of araneol. Spectrophotometric analysis of the photosynthetic pigments showed that UV-B radiation also increases the pigment content in treated plants. Another U V alteration is epidermal hair damage, as observed in SEM pictures of treated leaves. This combination of physiological and phytochemical effects may be interpreted as a plant response to UV-B stress.

  19. Changes in epicuticular flavonoids and photosynthetic pigments as a plant response to UV-B radiation

    International Nuclear Information System (INIS)

    Cuadra, P.; Harborne, J.B.

    1996-01-01

    Treatment of Gnaphalium vira-vira plants with UV-B radiation caused changes in plant growth and in plant chemistry. The leaf surface contained two O-methylated flavones, araneol and 7-O-methylaraneol. HPLC analysis showed that 20 days of UV-B radiation increased the synthesis of 7-O-methylaraneol at the expense of araneol. Spectrophotometric analysis of the photosynthetic pigments showed that UV-B radiation also increases the pigment content in treated plants. Another U V alteration is epidermal hair damage, as observed in SEM pictures of treated leaves. This combination of physiological and phytochemical effects may be interpreted as a plant response to UV-B stress

  20. An action spectrum for UV-B radiation and the rat lens.

    Science.gov (United States)

    Merriam, J C; Löfgren, S; Michael, R; Söderberg, P; Dillon, J; Zheng, L; Ayala, M

    2000-08-01

    To determine an action spectrum for UV-B radiation and the rat lens and to show the effect of the atmosphere and the cornea on the action spectrum. One eye of young female rats was exposed to 5-nm bandwidths of UV-B radiation (290, 295, 300, 305, 310, and 315 nm). Light scattering of exposed and nonexposed lenses was measured 1 week after irradiation. A quadratic polynomial was fit to the dose-response curve for each wave band. The dose at each wave band that produced a level of light scattering greater than 95% of the nonexposed lenses was defined as the maximum acceptable dose (MAD). Transmittance of the rat cornea was measured with a fiberoptic spectrophotometer. The times to be exposed to the MAD in Stockholm (59.3 degrees N) and La Palma (28 degrees N) were compared. Significant light scattering was detected after UV-B at 295, 300, 305, 310, and 315 nm. The lens was most sensitive to UV-B at 300 nm. Correcting for corneal transmittance showed that the rat lens is at least as sensitive to UV radiation at 295 nm as at 300 nm. The times to be exposed to the MAD at each wave band were greater in Stockholm than in La Palma, and in both locations the theoretical time to be exposed to the MAD was least at 305 nm. After correcting for corneal transmittance, the biological sensitivity of the rat lens to UV-B is at least as great at 295 nm as at 300 nm. After correcting for transmittance by the atmosphere, UV-B at 305 nm is the most likely wave band to injure the rat lens in both Stockholm and La Palma.

  1. Effect of enhanced UV-B radiation on motile microorganisms

    International Nuclear Information System (INIS)

    Haeder, D.P.

    1985-02-01

    The effect of slightly increased UV-B radiation was studied in four taxonomically very different microorganisms: the gliding prokaryotic cyanobacterium, Phormidium, the unicellular green alga Cosmarium, the flagellate Euglena and the cellular slime mold Dictyostelium. UV-B doses which can be expected as a result of a slight decrease of the protective ozone layer in the stratosphere, do not kill or damage the microorganisms visibly. However, such UV-B doses impair the development, motility and photoorientation of these organisms. Due to the inhibition of these physiological important parameters the organisms cannot respond adequately to the changing factors in their environment, which prevents the survival of the populations. (orig.) [de

  2. The effects of near-UV radiation on elasmobranch lens cytoskeletal actin.

    Science.gov (United States)

    Zigman, S; Rafferty, N S; Scholz, D L; Lowe, K

    1992-08-01

    The role of near-UV radiation as a cytoskeletal actin-damaging agent was investigated. Two procedures were used to analyse fresh smooth dogfish (Mustelus canis) eye lenses that were incubated for up to 22 hr in vitro, with elasmobranch Ringer's medium, and with or without exposure to a near-UV lamp (emission principally at 365 nm; irradiance of 2.5 mW cm-2). These were observed histologically using phalloidin-rhodamine specific staining and by transmission electron microscopy. In addition, solutions of purified polymerized rabbit muscle actin were exposed to the same UV conditions and depolymerization was assayed by ultracentrifugation and high-pressure liquid chromatography. While the two actins studied do differ very slightly in some amino acid sequences, they would react physically nearly identically. The results showed that dogfish lenses developed superficial opacities due to near-UV exposure. Whole mounts of lens epithelium exhibited breakdown of actin filaments in the basal region of the cells within 18 hr of UV exposure. TEM confirmed the breakdown of actin filaments due to UV exposure. SDS-PAGE and immunoblotting positively identified actin in these cells. Direct exposure of purified polymerized muscle actin in polymerizing buffer led to an increase in actin monomer of approximately 25% in the UV-exposed solutions within 3-18 hr, whether assayed by ultracentrifugation or HPLC. The above indicates that elasmobranch lens epithelial cells contain UV-labile actin filaments, and that near-UV radiation, as is present in the sunlit environment, can break down the actin structure in these cells. Furthermore, breakdown of purified polymerized muscle actin does occur due to near-UV light exposure.(ABSTRACT TRUNCATED AT 250 WORDS)

  3. Photosynthetic Performance of the Red Alga Pyropia haitanensis During Emersion, With Special Reference to Effects of Solar UV Radiation, Dehydration and Elevated CO2 Concentration.

    Science.gov (United States)

    Xu, Juntian; Gao, Kunshan

    2015-11-01

    Macroalgae distributed in intertidal zones experience a series of environmental changes, such as periodical desiccation associated with tidal cycles, increasing CO2 concentration and solar UVB (280-315 nm) irradiance in the context of climate change. We investigated how the economic red macroalga, Pyropia haitanensis, perform its photosynthesis under elevated atmospheric CO2 concentration and in the presence of solar UV radiation (280-400 nm) during emersion. Our results showed that the elevated CO2 (800 ppmv) significantly increased the photosynthetic carbon fixation rate of P. haitanensis by about 100% when the alga was dehydrated. Solar UV radiation had insignificant effects on the net photosynthesis without desiccation stress and under low levels of sunlight, but significantly inhibited it with increased levels of desiccation and sunlight intensity, to the highest extent at the highest levels of water loss and solar radiation. Presence of UV radiation and the elevated CO2 acted synergistically to cause higher inhibition of the photosynthetic carbon fixation, which exacerbated at higher levels of desiccation and sunlight. While P. haitanensis can benefit from increasing atmospheric CO2 concentration during emersion under low and moderate levels of solar radiation, combined effects of elevated CO2 and UV radiation acted synergistically to reduce its photosynthesis under high solar radiation levels during noon periods. © 2015 The American Society of Photobiology.

  4. Evaluating the effects of UV-B and UV-A irradiances on plant pigments, photosynthesis and growth in Glycine max L

    International Nuclear Information System (INIS)

    Middleton, E.H.M.

    1993-01-01

    Increasing penetration of UV-B radiation to the earth's surface resulting from stratospheric ozone depletion is an important environmental concern, but plant response to UV-B irradiation has been difficult to assess. The UV-A irradiance has not been specifically measured or controlled previously. The experimental UV-A was controlled in a series of three glasshouse experiments conducted under high photosynthetic photon flux (midday PPF ≥ 1200 μmol m -2 s -1 ). Low (LT) and High (HT) daily UV-B BE irradiances (10.7; 14.1 kJ m -2 ) were utilized in two experiments, whereas treatments with different UV-B BE :UV-A ratios ( BE :UV-A ratios

  5. Environmental radiation data, 7

    International Nuclear Information System (INIS)

    Nagaoka, Toshi; Sakamoto, Ryuichi; Saito, Kimiaki; Tsutsumi, Masahiro; Moriuchi, Shigeru

    1989-10-01

    The Environmental Radiation Physics Laboratory has conducted a large number of background radiation surveys in these years, aiming at the analysis of characteristics and behaviour of environmental radiation, the development of measurement techniques and instruments of environmental radiation, and the evaluation of environmental radiation dose. The environmental radiation data obtained by these surveys are useful for broad purposes as actual survey data. Therefore, it is desirable to make the recording media and the FORMAT of these data available for usual computers. In the light of this circumstance, these data were rearranged and recompiled systematically to meet the demand. This report mentions about the data obtained by the background radiation surveys in and around Tokyo performed during 1982 - 1988 using portable instruments, as well as the information necessary for the data handling. (author)

  6. Effects of enhanced UV-B radiation on Mentha spicata essential oils

    International Nuclear Information System (INIS)

    Karousou, R.; Grammatikopoulos, G.; Lanaras, T.; Manetas, Y.; Kokkini, S.

    1998-01-01

    In vitro propagated plantlets representing two distinct chemotypes of Mentha spicata, viz. plants producing essential oils rich in piperitone oxide and piperitenone oxide (chemotype I) and rich in carvone and dihydrocarvone (chemotype II), were grown in the field under ambient or ambient plus supplemental UV-B radiation, biologically equivalent to a 15% ozone depletion over Patras (38.3°N, 29.1°E), Greece. Enhanced UV-B radiation stimulated essential oil production in chemotype II substantially, while a similar, non-significant trend was observed in chemotype I. No effect was found on the qualitative composition of the essential oils, whereas the quantitative composition was slightly modified in chemotype I. This is the first investigation reporting an improved essential oil content under UV-B supplementation in aromatic plants under field conditions

  7. UV dosimetry in pollen of Pinus silvestris and stimulation studies of pollen tube growth after irradiation with UV and ionizing radiations

    International Nuclear Information System (INIS)

    Seibold, H.W.

    1976-01-01

    Pollen tube growth after exposure to UV- and ionizing radiation was investigated in Pinus silvestris as a function of different parameters. The preconditions for this are an exact UV dosimetry and the conversion of the UV dose of [erg] into [rad]. In spite of the fact that the calculation methods are theoretically already well known, the experimental determination of the absorbed energy dose, in particular the absorption factors, was complicated by the morphological structure of the pollen. The total-irradiation experiments showed that the stimulation and inhibition of pollen tube growth is highly dependent on the dose/dose rate region chosen. Stimulation is highly dependent on the dose rate after UV irradiation as well as after exposure to ionizing radiation. When the dose rates are very small, the values are practically the same as in non-irradiated controls; with increasing doses rate, stimulation increases, whereas at very high dose rates only growth inhibition is observed. After exposure to ionizing radiation, the quantum energy or wavelength of the radiation has no effect on tube growth; after UV irradiation, on the other hand, there is hardly any stimulation at short wavelengths. As these observations were not sufficient for drawing conclusions on the site of the stimulatory action, partial UV exposure with separate irradiation of the two nuclei and the cytoplasm was also carried out. Stimulation was found in each of the three irradiation sites; although nuclei and cytoplasma react differently and also differ in the initial reaction stages, there is always an anhancement of pollen tube growth. These findings are compared with some of the current hypotheses for an explanation of the stimulatory effect. (orig.) [de

  8. UV- Radiation Absorption by Ozone in a Model Atmosphere using ...

    African Journals Online (AJOL)

    UV- radiation absorption is studied through variation of ozone transmittance with altitude in the atmosphere for radiation in the 9.6μm absorption band using Goody's model atmosphere with cubic spline interpolation technique to improve the quality of the curve. The data comprising of pressure and temperature at different ...

  9. Impact of UV radiation on the physical properties of polypropylene ...

    African Journals Online (AJOL)

    The purpose of this study was to analyse the influence of simulated sun light radiation (xenon lamp) on physical properties of polypropylene (PP) nonwoven material, which is used for the production of agrotextiles. The research showed that the properties of row cover change when radiated with UV light. Tensile, tearing ...

  10. Effects of terrestrial UV radiation on selected outdoor materials: an interdisciplinary approach

    Science.gov (United States)

    Heikkilä, A.; Kazadzis, S.; Tolonen-Kivimäki, O.; Meinander, O.; Lindfors, A.; Lakkala, K.; Koskela, T.; Kaurola, J.; Sormanen, A.; Kärhä, P.; Naula-Iltanen, A.; Syrjälä, S.; Kaunismaa, M.; Juhola, J.; Ture, T.; Feister, U.; Kouremeti, N.; Bais, A.; Vilaplana, J. M.; Rodriguez, J. J.; Guirado, C.; Cuevas, E.; Koskinen, J.

    2009-08-01

    Modern polymeric materials possess an ever increasing potential in a large variety of outdoor objects and structures offering an alternative for many traditional materials. In outdoor applications, however, polymers are subject to a phenomenon called weathering. This is primarily observed as unwanted property changes: yellowing or fading, chalking, blistering, and even severe erosion of the material surface. One of the major weathering factors is UV radiation. In spring 2005, the Finnish Meteorological Institute with its research and industrial partners launched a five-year material research project named UVEMA (UV radiation Effects on MAterials). Within the framework of the project, a weathering network of seven European sites was established. The network extends from the Canary Islands of Spain (latitude 28.5°N) to the Lapland of Finland (latitude 67.4°N), covering a wide range of UV radiation conditions. Since autumn 2005, the sites of the network have been maintaining weathering platforms of specimens of different kinds of polymeric materials. At the same time, the sites have been maintaining their long-term monitoring programmes for spectrally resolved UV radiation. Within UVEMA, these data are used for explaining the differences between the degradation rates of the materials at each site and for correlating the UV conditions in accelerated ageing tests to those under the Sun. We will present the objectives of the UVEMA project aiming at deeper understanding of the ageing of polymers and more reliable assessments for their service life time. Methodologies adopted within the project and the first results of the project will be summarized.

  11. Measuring solar UV radiation with EBT radiochromic film

    International Nuclear Information System (INIS)

    Butson, Ethan T; Cheung Tsang; Yu, Peter K N; Butson, Martin J

    2010-01-01

    Ultraviolet radiation dosimetry has been performed with the use of a radiochromic film dosimeter called Gafchromic EBT for solar radiation exposure. The film changes from a clear colour to blue colour when exposed to ultraviolet radiation and results have shown that the colour change is reproducible within ±10% at 5 kJ m -2 UV exposure under various conditions of solar radiation. Parameters tested included changes in season (summer versus winter exposure), time of day, as well as sky conditions such as cloudy skies versus clear skies. As the radiochromic films' permanent colour change occurs in the visible wavelengths the film can be analysed with a desktop scanner with the most sensitive channel for analysis being the red component of the signal. Results showed that an exposure of 5 kJ m -2 (approximately 1 h exposure in full sun during summer) produced an approximate 0.28 change in the net OD when analysed in reflection mode on the desktop scanner which is significant darkening. The main advantages of this film type, and thus the new EBT2 film which has replaced EBT for measurement of UV exposure, is the visible colour change and thus easy analysis using a desktop scanner, its uniformity in response and its robust physical strength for use in outside exposure situations. (note)

  12. Thermoluminescence induced by UV radiation in ZrO2-La2O3 and ZrO2-CeO2 mixed systems

    International Nuclear Information System (INIS)

    Estrada G, R.; Salas C, P.; Mendoza A, D.; Gonzalez, P.R.

    2002-01-01

    Nowadays, the development of catalytic materials by means of pure or mixed oxides mainly as TiO 2 , Al 2 O 3 , ZrO 2 , La 2 O 3 and CeO 2 used as support or active phases, are widely used in oxidation-reduction reactions in the chemical industry and in petroleum refining processes. the cerium and lanthanum oxides in zircon, have been studied recently in processes for reduction of pollutant gases (SO x , NO x , etc.). They result very interesting for resolving the problems of environmental pollution. Moreover, it has been observed that some of these materials are highly sensitive to ultraviolet radiation (UV), characteristic that can be took in advantage for detecting this type of radiation. In this work the preliminary obtained results on the thermoluminescent response (Tl) induced by the UV radiation in ZrO 2 -La 2 O 3 and ZrO 2 -CeO 2 mixed systems obtained by the sol-gel method are presented. The results show that the first system has a high sensitivity to UV radiation with a Tl curve composed by three peaks, two ones of greater intensity located in 70 and 140 Centigrade degrees. Likewise it was observed that for both materials, the peak located at low temperature is fadeout in few minutes after irradiation, while that the second one peak presented very good stability, resulting promising for dosimetric applications in UV radiation fields. (Author)

  13. Five years of solar UV-radiation monitoring in Sweden

    Energy Technology Data Exchange (ETDEWEB)

    Josefsson, Weine

    1996-10-01

    A network of five stations measuring the solar UV-radiation has been operated for about five years. Data are presented as plotted time-series of monthly and yearly values for the sites. A general climatology can be deduced from these data. Daily and hourly maximum values are shown for each month as indicators of the potential extreme exposure levels. The large annual variation at high latitudes is easily seen in the data set. This illustrates the importance of the solar elevation on the level of the UV-irradiance. Influence of cloud variation and of larger changes in ozone is also detectable. A few examples of the daily variation also show the strong solar elevation dependence of the UV-irradiance. The quantity and unit of the UV-radiation in this presentation is CIE-weighted irradiance expressed as MED (minimum erythermal dose), where one MED equals 210 Jm{sup -2}. The values have been recomputed to refer to the international intercomparison of broad-band meters in Helsinki in 1995. In the following named WMO-STUK 1995 scale. As will be seen there are many sources of error and detailed studies are prevented by the large uncertainty connected with these data. Due to the short period of the record and the low accuracy no attempt to study trends is done. 6 refs, 27 figs, 4 tabs

  14. An assessment of ozone levels, UV radiation and their occupational health hazard estimation during photocopying operation

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Bhupendra Pratap, E-mail: bpsingh0783@gmail.com; Kumar, Amit; Singh, Deepak; Punia, Monika; Kumar, Krishan; Jain, Vinod Kumar

    2014-06-30

    Highlights: • First quantitative report of ozone level and UV radiation emission from photocopier. • Ozone production is directly proportional with intensity of photocopy operation. • Ozone level from ground floor is significantly higher than basement photocopier. • Ozone production and UV radiation studied has less correlation during photocopy. • Health hazard issue has been evaluated for effect of UV radiation in terms of SED. - Abstract: This study investigates the levels of ozone concentration along with an ultraviolet (UV) and visible spectral radiation at eight photocopy centers in an academic institute, Delhi. Sampling was done in two types of locations, i.e., basement photocopy centers (BPC) and ground floor photocopy centers (GPC) for 8 h. Measurements of levels of ozone, UV and visible radiation were done by ozone analyzer, UV radiometer and Field spectra instrument, respectively. Results show that the hourly mean concentration of ozone was observed to be in the range of 1.8–10.0 ppb and 5.3–45.8 ppb for BPC and GPC, respectively. In terms UV radiations, energy lies between 5.0 × 10{sup −3} and 7.0 × 10{sup −3} mW/cm{sup 2} for ultraviolet A (UVA), 1.0 × 10{sup −3} and 2.0 × 10{sup −3} mW/cm{sup 2} for ultraviolet B (UVB) and 6.0 × 10{sup −3} and 8.0 × 10{sup −3} mW/cm{sup 2} for ultraviolet C (UVC). Correlation between the UV radiations and ozone production observed was statistically insignificant. To know the health hazard occurred to the workers, the standard erythema dose (SED) value was calculated for emitting UV radiation. The SED was estimated to be in the range of 0.02–0.04 and 0.02–0.32 for direct and indirect methods which is less than the guideline prescribed by Commission Internationale del’ Eclairage (CIE). In nutshell, person involved in photocopy operation for their livelihood must be trained and should have knowledge for the long term gradual build up health problems due to ozone and UV production from

  15. Radiative symmetry breaking from interacting UV fixed points

    DEFF Research Database (Denmark)

    Abel, Steven; Sannino, Francesco

    2017-01-01

    It is shown that the addition of positive mass-squared terms to asymptotically safe gauge-Yukawa theories with perturbative UV fixed points leads to calculable radiative symmetry breaking in the IR. This phenomenon, and the multiplicative running of the operators that lies behind it, is akin...

  16. Rapid bioelectric reaction of elodea leaf cells to the UV radiation

    International Nuclear Information System (INIS)

    Aliev, D.A.; Mamedov, T.G.; Akhmedov, I.S.; Khalilov, R.I.

    1984-01-01

    It has been established that changes of membrane potential (MP) of elodea leaf cells in the UV radiation are manifested in a form of rapid response reaction, which is similar to an action potential. At present a lot of new data confirming the existence of electrogenic proton pump on plasmalemma plant cells is making their appearance. The plant cell membrane potential consists of two components: equilibrium( passive) potential and potential created by an electrogenic proton pump. A contribution of the second component to the elodea leaf cell MP is considerable and constitutes more than a half of the total MP. Constant values of membrane conductivity and intracell electric bonds in the process of depolarization development and after MP recovery testify to the fact, that UV radiation does not effect upon the MP passive component. High degree of depolarization and its strong dependence on medium pH and also the observed effect independence on potassium and sodium ions presence in the external medium testify to the fact that UV radiation ingenuously inactivates electrogenic proton pumps

  17. Long-term effects of elevated UV-B radiation on photosynthesis and ultrastructure of Eriophorum russeolum and Warnstorfia exannulata

    International Nuclear Information System (INIS)

    Haapala, Jaana K.; Moersky, Sami K.; Saarnio, Sanna; Suokanerva, Hanne; Kyroe, Esko; Silvola, Jouko; Holopainen, Toini

    2010-01-01

    The depletion of stratospheric ozone above the Arctic regions may increase the amount of UV-B radiation to which the northern ecosystems are exposed. In this paper, we examine the hypothesis that supplemental UV-B radiation may affect the growth rate and photosynthesis of boreal peatland plants and could thereby affect the carbon uptake of these ecosystems. In this study, we report the effects of 3-year exposure to elevated UV-B radiation (46% above ambient) on the photosynthetic performance and ultrastructure of a boreal sedge Eriophorum russeolum and a moss Warnstorfia exannulata. The experiment was conducted on a natural fen ecosystem at Sodankylae in northern Finland. The effects of UV-B radiation on the light response of E. russeolum CO 2 assimilation and the maximal photochemical efficiency of photosystem II in a dark-adapted state (F v /F m ) were measured in the field. In addition, the effect of supplemental UV-B radiation on organelles of photosynthetic cells was studied by electron microscopy. The UV-B treatment had no effect on the CO 2 assimilation rate of either species, nor did it affect the structure of the cell organelles. On chlorophyll fluorescence, the UV-B exposure had only a temporary effect during the third exposure year. Our results suggested that in a natural ecosystem, even long-term exposure to reasonably elevated UV-B radiation levels does not affect the photosynthesis of peatland plants. - Research highlights: →Eriophorum russeolum and Warnstorfia exannulata are resistant to UV-B radiationUV-B exposure does not affect the growth or photosynthesis of E. russeolum →Long-term UV-B exposure has no effect on the ultrastructure of E. russeolum

  18. Water and Wastewater Disinfection with Peracetic Acid and UV Radiation and Using Advanced Oxidative Process PAA/UV

    OpenAIRE

    Beber de Souza, Jeanette; Queiroz Valdez, Fernanda; Jeranoski, Rhuan Felipe; Vidal, Carlos Magno de Sousa; Cavallini, Grasiele Soares

    2015-01-01

    The individual methods of disinfection peracetic acid (PAA) and UV radiation and combined process PAA/UV in water (synthetic) and sanitary wastewater were employed to verify the individual and combined action of these advanced oxidative processes on the effectiveness of inactivation of microorganisms indicators of fecal contamination E. coli, total coliforms (in the case of sanitary wastewater), and coliphages (such as virus indicators). Under the experimental conditions investigated, doses o...

  19. A comparative approach of methylparaben photocatalytic degradation assisted by UV-C, UV-A and Vis radiations.

    Science.gov (United States)

    Doná, Giovanna; Dagostin, João Luiz Andreoti; Takashina, Thiago Atsushi; de Castilhos, Fernanda; Igarashi-Mafra, Luciana

    2018-05-01

    Due to the widespread use of methylparaben (MEP) and its high chemical stability, it can be found in wastewater treatment plants and can act as an endocrine disrupting compound. In this study, the photocatalytic degradation and mineralization of MEP solutions were evaluated under UV-A, UV-C and Vis radiations in the presence of the photocatalyst TiO 2 . In this sense, the effects of the catalyst load, pH and MEP initial concentration were studied. Remarkably higher reaction rates and total photodegradation were achieved in systems assisted by UV-C radiation. The complete degradation was achieved after 60 min of reaction using the MEP concentration of 30 mg L -1 at pH 9 and 500 mg L -1 TiO 2 . The experimental data apparently followed a Langmuir-Hinshelwood kinetic model, which could predict 88-98% of the reaction behavior. For the best photodegradation condition, the model predicted an apparent reaction rate constant (k app ) equal to 0.0505 min -1 and an initial reaction rate of 1.5641 mg (L min) -1 . Mineralization analyses showed high removal for MEP and derived compounds from the initial solution when using UV-C after 90 min of reaction. The lower toxicity was also confirmed by in vivo tests using MEP solutions previously treated by photocatalysis.

  20. UV light-induced survival response in a highly radiation-resistant isolate of the Moraxella-acinetobacter group

    International Nuclear Information System (INIS)

    Keller, L.C.; Thompson, T.L.; Maxcy, R.B.

    1982-01-01

    A highly radiation-resistant member of the Moraxella-Acinetobacter group, isolate 4, obtained from meat, was studied to determine the effect of preexposure to UV radiation on subsequent UV light resistance. Cultures that were preexposed to UV light and incubated for a short time in plate count broth exhibited increased survival of a UV light challenge dose. This response was inhibited in the presence of chloramphenicol. Frequencies of mutation to streptomycin, trimethoprim, and sulfanilamide resistance remained the same after the induction of this survival response and were not altered by treatment with mutagens, with the exception of mutation to streptomycin resistance after γ-irradiation or nitrosoguanidine or methyl methane sulfonate treatment. The results indicated that isolate 4 has a UV light-inducible UV light resistance mechanism which is not associated with increased mutagenesis. The characteristics of the radiation resistance response in this organism are similar to those of certain other common food contaminants. Therefore, considered as part of the total microflora of meat, isolate 4 and the other radiation-resistant Moraxella-Acinetobacter isolates should not pose unique problems in a proposed radappertizaton process

  1. Combined impact of solar UV-B radiation and selenium treatment on respiratory potential in pumpkins (Cucurbita pepo L.)

    International Nuclear Information System (INIS)

    Germ, M.

    2005-01-01

    The effects of ambient and filtered solar UV-B radiation and of selenium treatment on respiratory potential measured by electron transport system (ETS) activity in pumpkins, Cucurbita pepo L. were studied. Measurements were conducted three times in the growth period. Solar UV-B radiation decreased ETS activity in plants, regardless selenium treatment. The results suggested that the solar UV-B radiation impaired flow of electrons in the respiratory chain. Selenium decreased ETS activity in plants exposed to solar UV-B radiation in the end of the vegetation period

  2. Environmental Radiation Data

    Data.gov (United States)

    U.S. Environmental Protection Agency — Environmental Radiation Data (ERD) is an electronic and print journal compiled and distributed quarterly by the Office of Radiation and Indoor Air's National Air and...

  3. Effect of ionizing (gamma and non-ionizing (UV radiation on the development of Trichogramma euproctidis (Hymenoptera: Trichogrammatidae

    Directory of Open Access Journals (Sweden)

    Tuncbilek Aydin S.

    2012-01-01

    Full Text Available The potential of using gamma and ultraviolet radiation as an alternative treatment to increase the efficiency of Trichogramma euproctidis (Girault 1911 (Hymenoptera: Trichogrammatidae was investigated in the laboratory. The developmental and adult stages of T. euproctidis were exposed to gamma radiation of different doses (0-30 Gy and ultraviolet radiation of 254 nm wavelengths (UV-C for different durations (0-10 min to assess their effect on each of the instars and their potential in breaking the developmental cycle of the egg parasitoid. The LD50 values for eggs, prepupae, pupae and adults were 8.1, 10.0, 22.7 and 9.5 Gy for gamma radiation and 9.5, 0.12, 2.0 and 11.9 min for UV radiation, respectively. The pupa and adult stages were more radioresistant to both gamma and UV radiation. The most interesting and unexpected result obtained for the prepupal stage was that UV radiation has a greater effect on prepupal stages than gamma radiation.

  4. UV reflectance is associated with environmental conditions in Palaearctic Pieris napi (Lepidoptera: Pieridae).

    Science.gov (United States)

    Stella, David; Pecháček, Pavel; Meyer-Rochow, Victor Benno; Kleisner, Karel

    2018-06-01

    The subject of our investigation was the visual features of wing color with special focus on the UV reflectance in the green-veined white butterfly (Pieris napi). Previous studies had concluded that UV reflectance on dorsal wing surfaces is found only in the female P. napi. Based on UV sensitive photography, we analyzed a correlation between 12 geographic and environmental factors and UV reflectance patterns on 3 patches on the forewings of 407 P. napi specimens from the Palaearctic region. Results had shown that females significantly differ from males: they exhibit a 25% higher UV reflectance. To investigate whether and how UV reflectance levels on the forewings and hindwings of both sexes are influenced by the environment, we performed a principal component analysis (PCA) with several environmental variables. For several variables (in particular, latitude and longitude, mean annual temperature and precipitation, and temperature annual range and altitude), the generalized linear model (GLM) model revealed a significant correlation in both sexes. This suggests a link between UV reflectance levels and the environment and distribution of P. napi. We found that stronger UV reflectance is associated with generally more hostile environments and concluded that large-scale environmental factors influence the UV reflectance on the forewings of both male and female green-veined white butterflies. © 2016 Institute of Zoology, Chinese Academy of Sciences.

  5. Sensitivity to UV radiation in early life stages of the Mediterranean sea urchin Sphaerechinus granularis (Lamarck)

    International Nuclear Information System (INIS)

    Nahon, Sarah; Castro Porras, Viviana A.; Pruski, Audrey M.; Charles, Francois

    2009-01-01

    The sea urchin Sphaerechinus granularis was used to investigate the impact of relevant levels of UV-B radiation on the early life stages of a common Mediterranean free spawning benthic species. Sperm, eggs and embryos were exposed to a range of UV radiation doses. The resulting endpoints were evaluated in terms of fertilisation success, development and survival rates. Above a weighted UV radiation dose of 0.0029 kJ m -2 , fertilisation capability of irradiated sperm decreased rapidly. The exposure of the eggs to 0.0175 kJ m -2 and more led to delayed and inhibited development with ensuing embryonic morphological abnormalities. One-day old larvae remained strongly sensitive to UV radiation as shown by the 50% decrease of the larval survival rate for a dose of 0.025 kJ m -2 UVR. The elevated sensitivity of embryos to experimental UVR went along with a lack of significant amount of sunscreen compounds (e.g., mycosporine-like amino acids) in the eggs. The present results demonstrated that gamete viability and embryonic development may be significantly impaired by solar UV radiation in S. granularis, compromising in this way the reproduction of the species. Unless adaptive behavioural reproductive strategies exist, the influence of ambient UV radiation appears as a selective force for population dynamics of broadcast spawners in the shallow benthic Mediterranean environment

  6. Sensitivity to UV radiation in early life stages of the Mediterranean sea urchin Sphaerechinus granularis (Lamarck)

    Energy Technology Data Exchange (ETDEWEB)

    Nahon, Sarah [UPMC Univ Paris 06, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); CNRS, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); Castro Porras, Viviana A. [UPMC Univ Paris 06, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); Pruski, Audrey M. [UPMC Univ Paris 06, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); CNRS, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); Charles, Francois [UPMC Univ Paris 06, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France); CNRS, UMR 7621, LOBB, Observatoire Oceanologique, F-66651, Banyuls/mer (France)], E-mail: charles@obs-banyuls.fr

    2009-03-01

    The sea urchin Sphaerechinus granularis was used to investigate the impact of relevant levels of UV-B radiation on the early life stages of a common Mediterranean free spawning benthic species. Sperm, eggs and embryos were exposed to a range of UV radiation doses. The resulting endpoints were evaluated in terms of fertilisation success, development and survival rates. Above a weighted UV radiation dose of 0.0029 kJ m{sup -2}, fertilisation capability of irradiated sperm decreased rapidly. The exposure of the eggs to 0.0175 kJ m{sup -2} and more led to delayed and inhibited development with ensuing embryonic morphological abnormalities. One-day old larvae remained strongly sensitive to UV radiation as shown by the 50% decrease of the larval survival rate for a dose of 0.025 kJ m{sup -2} UVR. The elevated sensitivity of embryos to experimental UVR went along with a lack of significant amount of sunscreen compounds (e.g., mycosporine-like amino acids) in the eggs. The present results demonstrated that gamete viability and embryonic development may be significantly impaired by solar UV radiation in S. granularis, compromising in this way the reproduction of the species. Unless adaptive behavioural reproductive strategies exist, the influence of ambient UV radiation appears as a selective force for population dynamics of broadcast spawners in the shallow benthic Mediterranean environment.

  7. Response of antioxidant defense system to laser radiation apical meristem of Isatis indigotica seedlings exposed to UV-B.

    Science.gov (United States)

    Chen, Yi-Ping

    2009-07-01

    To determine the response of antioxidant defense system to laser radiation apical meristem of Isatis indigotica seedlings, Isatis indigotica seedlings were subjected to UV-B radiation (10.08 kJ m(-2)) for 8 h day(-1) for 8 days (PAR, 220 micromol m(-2) s(-1)) and then exposed to He-Ne laser radiation (633 nm; 5.23 mW mm(-2); beam diameter: 1.5 mm) for 5 min each day without ambient light radiation. Changes in free radical elimination systems were measured, the results indicate that: (1) UV-B radiation enhanced the concentration of Malondialdahyde (MDA) and decreased the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in seedlings compared with the control. The concentration of MDA was decreased and the activities of SOD, CAT and POD were increased when seedlings were subjected to elevated UV-B damage followed by laser; (2) the concentration of UV absorbing compounds and proline were increased progressively with UV-B irradiation, laser irradiation and He-Ne laser irradiation plus UV-B irradiation compared with the control. These results suggest that laser radiation has an active function in repairing UV-B-induced lesions in seedlings.

  8. Increased UV-B radiation reduces N2-fixation in tropical leguminous crops

    International Nuclear Information System (INIS)

    Anupa Singh

    1997-01-01

    Net photosynthesis, leaf area, biomass, and number, size and activity of nodules were examined in three leguminous plants subjected under field conditions to supplemental UV-B radiation equivalent to a 15% ozone depletion at 25 degrees N latitude. Enhanced UV-B radiation adversely affected the net photosynthetic rate, growth characteristics and nodule activity in all three species. Maximum reduction in net photosynthesis occurred in Phaseolus mungo cv. Pant U-30, whereas the greatest reduction in nitrogenase activity occurred in Vigna radiata. (author)

  9. Natural Microbial UV Radiation Filters - Mycosporine-like Amino Acids

    Czech Academy of Sciences Publication Activity Database

    Řezanka, Tomáš; Temina, M.; Tolstikov, A. G.; Dembitsky, V. M.

    2004-01-01

    Roč. 49, č. 4 (2004), s. 339-352 ISSN 0015-5632 Institutional research plan: CEZ:AV0Z5020903 Keywords : uv radiation * maas * ultraviolet-b Subject RIV: EE - Microbiology, Virology Impact factor: 1.034, year: 2004

  10. UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii.

    Science.gov (United States)

    Fulgentini, Lorenzo; Passini, Valerio; Colombetti, Giuliano; Miceli, Cristina; La Terza, Antonietta; Marangoni, Roberto

    2015-08-01

    The psychrophilic ciliate Euplotes focardii inhabits the shallow marine coastal sediments of Antarctica, where, over millions of years of evolution, it has reached a strict molecular adaptation to such a constant-temperature environment (about -2 °C). This long evolution at sub-zero temperatures has made E. focardii unable to respond to heat stress with the activation of its heat shock protein (hsp) 70 genes. These genes can, however, be expressed in response to other stresses, like the oxidative one, thus indicating that the molecular adaptation has exclusively altered the heat stress signaling pathways, while it has preserved hsp70 gene activation in response to other environmental stressors. Since radiative stress has proved to be affine to oxidative stress in several organisms, we investigated the capability of UV radiation to induce hsp70 transcription. E. focardii cell cultures were exposed to several different irradiation regimes, ranging from visible only to a mixture of visible, UV-A and UV-B. The irradiation values of each spectral band have been set to be comparable with those recorded in a typical Antarctic spring. Using Northern blot analysis, we measured the expression level of hsp70 immediately after irradiation (0-h-labeled samples), 1 h, and 2 h from the end of the irradiation. Surprisingly, our results showed that besides UV radiation, the visible light was also able to induce hsp70 expression in E. focardii. Moreover, spectrophotometric measurements have revealed no detectable endogenous pigments in E. focardii, making it difficult to propose a possible explanation for the visible light induction of its hsp70 genes. Further research is needed to conclusively clarify this point.

  11. DSMC simulation of two-phase plume flow with UV radiation

    Energy Technology Data Exchange (ETDEWEB)

    Li, Jie; Liu, Ying; Wang, Ning; Jin, Ling [College of Aerospace Science and Engineering, National University of Defense Technology, Changsha, Hunan, 410073 (China)

    2014-12-09

    Rarefied gas-particle two-phase plume in which the phase of particles is liquid or solid flows from a solid propellant rocket of hypersonic vehicle flying at high altitudes, the aluminum oxide particulates not only impact the rarefied gas flow properties, but also make a great difference to plume radiation signature, so the radiation prediction of the rarefied gas-particle two-phase plume flow is very important for space target detection of hypersonic vehicles. Accordingly, this project aims to study the rarefied gas-particle two-phase flow and ultraviolet radiation (UV) characteristics. Considering a two-way interphase coupling of momentum and energy, the direct simulation Monte Carlo (DSMC) method is developed for particle phase change and the particle flow, including particulate collision, coalescence as well as separation, and a Monte Carlo ray trace model is implemented for the particulate UV radiation. A program for the numerical simulation of the gas-particle two-phase flow and radiation in which the gas flow nonequilibrium is strong is implemented as well. Ultraviolet radiation characteristics of the particle phase is studied based on the calculation of the flow field coupled with the radiation calculation, the radiation model for different size particles is analyzed, focusing on the effects of particle emission, absorption, scattering as well as the searchlight emission of the nozzle. A new approach may be proposed to describe the rarefied gas-particle two-phase plume flow and radiation transfer characteristics in this project.

  12. DSMC simulation of two-phase plume flow with UV radiation

    Science.gov (United States)

    Li, Jie; Liu, Ying; Wang, Ning; Jin, Ling

    2014-12-01

    Rarefied gas-particle two-phase plume in which the phase of particles is liquid or solid flows from a solid propellant rocket of hypersonic vehicle flying at high altitudes, the aluminum oxide particulates not only impact the rarefied gas flow properties, but also make a great difference to plume radiation signature, so the radiation prediction of the rarefied gas-particle two-phase plume flow is very important for space target detection of hypersonic vehicles. Accordingly, this project aims to study the rarefied gas-particle two-phase flow and ultraviolet radiation (UV) characteristics. Considering a two-way interphase coupling of momentum and energy, the direct simulation Monte Carlo (DSMC) method is developed for particle phase change and the particle flow, including particulate collision, coalescence as well as separation, and a Monte Carlo ray trace model is implemented for the particulate UV radiation. A program for the numerical simulation of the gas-particle two-phase flow and radiation in which the gas flow nonequilibrium is strong is implemented as well. Ultraviolet radiation characteristics of the particle phase is studied based on the calculation of the flow field coupled with the radiation calculation, the radiation model for different size particles is analyzed, focusing on the effects of particle emission, absorption, scattering as well as the searchlight emission of the nozzle. A new approach may be proposed to describe the rarefied gas-particle two-phase plume flow and radiation transfer characteristics in this project.

  13. Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation.

    Science.gov (United States)

    Beblo, Kristina; Douki, Thierry; Schmalz, Gottfried; Rachel, Reinhard; Wirth, Reinhard; Huber, Harald; Reitz, Günther; Rettberg, Petra

    2011-11-01

    In this study, we investigated the ability of several (hyper-) thermophilic Archaea and phylogenetically deep-branching thermophilic Bacteria to survive high fluences of monochromatic UV-C (254 nm) and high doses of ionizing radiation, respectively. Nine out of fourteen tested microorganisms showed a surprisingly high tolerance against ionizing radiation, and two species (Aquifex pyrophilus and Ignicoccus hospitalis) were even able to survive 20 kGy. Therefore, these species had a comparable survivability after exposure to ionizing radiation such as Deinococcus radiodurans. In contrast, there was nearly no difference in survival of the tested strains after exposure to UV-C under anoxic conditions. If the cells had been dried in advance of UV-C irradiation, they were more sensitive to UV-C radiation compared with cells irradiated in liquid suspension; this effect could be reversed by the addition of protective material like sulfidic ores before irradiation. By exposure to UV-C, photoproducts were formed in the DNA of irradiated Archaea and Bacteria. The distribution of the main photoproducts was species specific, but the amount of the photoproducts was only partly dependent on the applied fluence. Overall, our results show that tolerance to radiation seems to be a common phenomenon among thermophilic and hyperthermophilic microorganisms.

  14. Relationship of photosynthetic carbon fixation with environmental changes in the Jiulong River estuary of the South China Sea, with special reference to the effects of solar UV radiation

    International Nuclear Information System (INIS)

    Li Gang; Gao Kunshan; Yuan Dongxing; Zheng Ying; Yang Guiyuan

    2011-01-01

    Highlights: → C-fixation is the highest in turbidity front, though UV resulted in higher inhibition. → Increased availability of CO 2 appeared to stimulate photosynthetic machinery. → Osmotic stress made phytoplankton more sensitive to UV. - Abstract: Phytoplankton cells in estuary waters usually experience drastic changes in chemical and physical environments due to mixing of fresh and seawaters. In order to see their photosynthetic performance in such dynamic waters, we measured the photosynthetic carbon fixation by natural phytoplankton assemblages in the Jiulong River estuary of the South China Sea during April 24-26 and July 24-26 of 2008, and investigated its relationship with environmental changes in the presence or the absence of UV radiation. Phytoplankton biomass (Chl a) decreased sharply from the river-mouth to seawards (17.3-2.1 μg L -1 ), with the dominant species changed from chlorophytes to diatoms. The photosynthetic rate based on Chl a at noon time under PAR-alone increased from 1.9 μg C (μg Chl a) -1 L -1 in low salinity zone (SSS -1 L -1 in turbidity front (SSS within 10-20), and then decreased to 2.1 μg C (μg Chl a) -1 L -1 in mixohaline zone (SSS > 20); accordingly, the carbon fixation per volume of seawater increased from 12.8 to 149 μg C L -1 h -1 , and decreased to 14.3 μg C L -1 h -1 . Solar UVR caused the inhibition of carbon fixation in surface water of all the investigated zones, by 39% in turbidity area and 7-10% in freshwater or mixohaline zones. In the turbidity zone, higher availability of CO 2 could have enhanced the photosynthetic performance; while osmotic stress might be responsible for the higher sensitivity of phytoplankton assemblages to solar UV radiation.

  15. Photomorphogenetic responses to UV radiation and short-term red light in lettuce seedlings

    International Nuclear Information System (INIS)

    Kobzar, E.F.; Kreslavski, V.D.; Muzafarov, E.N.

    1998-01-01

    Effects of red light (R), far-red light (FR) and UV radiation on growth and greening of lettuce seedlings (Latuca sativa L., cv. Berlinskii) have been investigated. UV-B and UV-C inhibited hypocotyl elongation and stimulated cotyledonary growth. R in combination with UV-B and UV-C partly eliminated these effects, but FR increased those and reversed the R effect. Chlorophyll accumulation was inhibited by UV-B and UV-C. In comparison with cotyledonary growth, R strengthened the UV inhibitory effect, and FR reversed this effect of R. Thus, UV and phytochrome system modify the effects of each other on hypocotyl and leaf growth in lettuce seedlings depending on the level of active phytochrome formed

  16. Light scattering on PHA granules protects bacterial cells against the harmful effects of UV radiation.

    Science.gov (United States)

    Slaninova, Eva; Sedlacek, Petr; Mravec, Filip; Mullerova, Lucie; Samek, Ota; Koller, Martin; Hesko, Ondrej; Kucera, Dan; Marova, Ivana; Obruca, Stanislav

    2018-02-01

    Numerous prokaryotes accumulate polyhydroxyalkanoates (PHA) in the form of intracellular granules. The primary function of PHA is the storage of carbon and energy. Nevertheless, there are numerous reports that the presence of PHA granules in microbial cells enhances their stress resistance and fitness when exposed to various stress factors. In this work, we studied the protective mechanism of PHA granules against UV irradiation employing Cupriavidus necator as a model bacterial strain. The PHA-accumulating wild type strain showed substantially higher UV radiation resistance than the PHA non-accumulating mutant. Furthermore, the differences in UV-Vis radiation interactions with both cell types were studied using various spectroscopic approaches (turbidimetry, absorption spectroscopy, and nephelometry). Our results clearly demonstrate that intracellular PHA granules efficiently scatter UV radiation, which provides a substantial UV-protective effect for bacterial cells and, moreover, decreases the intracellular level of reactive oxygen species in UV-challenged cells. The protective properties of the PHA granules are enhanced by the fact that granules specifically bind to DNA, which in turn provides shield-like protection of DNA as the most UV-sensitive molecule. To conclude, the UV-protective action of PHA granules adds considerable value to their primary storage function, which can be beneficial in numerous environments.

  17. Subthreshold UV radiation-induced peroxide formation in cultured corneal epithelial cells: the protective effects of lactoferrin

    International Nuclear Information System (INIS)

    Shimmura, Shigeto; Suematsu, Makoto; Shimoyama, Masaru; Oguchi, Yoshihisa; Ishimura, Yuzuru

    1996-01-01

    Acute exposure to suprathreshold ultraviolet B radiation (UV-B) is known to cause photokeratitis resulting from the necrosis and shedding of corneal epithelial cells. However, the corneal effects of low dose UV-B in the environmental range is less clear. In this study, subthreshold UV-B was demonstrated to cause non-necrotic peroxide formation in cultured corneal epithelial cells, which was attenuated by the major tear protein lactoferrin. Intracellular oxidative insults and cell viability of rabbit corneal epithelial cells (RCEC) were assessed by dual-color digital microfluorography using carboxydichlorofluorescin (CDCFH) diacetate bis (acetoxymethyl) ester, a hydroperoxide-sensitive fluoroprobe, and propidium iodode (PI) respectively. The magnitude of UV-induced oxidative insults was calibrated by concentrations of exogenously applied H 2 O 2 which evoke compatible levels of CDCFH oxidation. Exposure of RCEC to low-dose UV-B (2.0 mJ cm -2 at 313 nm, 10.0 mJ cm -2 total UV-B) caused intracellular oxidative changes which were equivalent to those elicited by 240 μM hydrogen peroxide under the conditions of the study. The changes were dose dependent, non-necrotic, and were partially inhibited by lactoferrin ( 1 mg ml -1 ) but not by iron-saturated lactoferrin. Pretreatment with deferoxamine (2 mΜ) or catalase (100 U ml -1 ) also attenuated the UV-induced oxidative stress. The results indicate that UV-B comparable to solar irradiation levels causes significant intracellular peroxide formation in corneal epithelial cells, and that lactoferrin in tears may have a physiological role in protecting the corneal epithelium from solar UV irradiation. (Author)

  18. Effects of lanthanum(III) on nitrogen metabolism of soybean seedlings under elevated UV-B radiation.

    Science.gov (United States)

    Cao, Rui; Huang, Xiao-hua; Zhou, Qing; Cheng, Xiao-ying

    2007-01-01

    The hydroponic culture experiments of soybean bean seedlings were conducted to investigate the effect of lanthanum (La) on nitrogen metabolism under two different levels of elevated UV-B radiation (UV-B, 280-320 nm). The whole process of nitrogen metabolism involves uptake and transport of nitrate, nitrate assimilation, ammonium assimilation, amino acid biosynthesis, and protein synthesis. Compared with the control, UV-B radiation with the intensity of low level 0.15 W/m2 and high level 0.45 W/m2 significantly affected the whole nitrogen metabolism in soybean seedlings (p nitrogen-metabolism-related enzymes, such as: nitrate reductase (NR) to the nitrate reduction, glutamine systhetase (GS) and glutamine synthase (GOGAT) to the ammonia assimilation, while it increased the content of free amino acids and decreased that of soluble protein as well. The damage effect of high level of UV-B radiation on nitrogen metabolism was greater than that of low level. And UV-B radiation promoted the activity of the anti-adversity enzyme glutamate dehydrogenase (GDH), which reduced the toxicity of excess ammonia in plant. After pretreatment with the optimum concentration of La (20 mg/L), La could increase the activity of NR, GS, GOGAT, and GDH, and ammonia assimilation, but decrease nitrate and ammonia accumulation. In conclusion, La could relieve the damage effect of UV-B radiation on plant by regulating nitrogen metabolism process, and its alleviating effect under low level was better than that under the high one.

  19. Effects of increasing UV-B radiation and atmospheric CO2 on photosynthesis and growth: implications for terrestrial ecosystems

    International Nuclear Information System (INIS)

    Sullivan, J.H.

    1997-01-01

    Increases in UV-B radiation reaching the earth as a result of stratospheric ozone depletion will most likely accompany increases in atmospheric CO 2 concentrations. Many studies have examined the effects of each factor independently, but few have evaluated the combined effects of both UV-B radiation and elevated CO 2 . In general the results of such studies have shown independent effects on growth or seed yield. Although interspecific variation is large, high levels of UV-B radiation tends to reduce plant growth in sensitive species, while CO 2 enrichment tends to promote growth in most C 3 species. However, most previous studies have not looked at temporal effects or at the relationship between photosynthetic acclimation to CO 2 and possible photosynthetic limitations imposed by UV-B radiation. Elevated CO 2 may provide some protection against UV-B for some species. In contrast, UV-B radiation may limit the ability to exploit elevated CO 2 in other species. Interactions between the effects of CO 2 enrichment and UV-B radiation exposure have also been shown for biomass allocation. Effects on both biomass allocation and photosynthetic acclimation may be important to ecosystem structure in terms of seedling establishment, competition and reproductive output. Few studies have evaluated ecosystem processes such as decomposition or nutrient cycling. Interactive effects may be subtle and species specific but should not be ignored in the assessment of the potential impacts of increases in CO 2 and UV-B radiation on plants. (author)

  20. Photoprotectant improves photostability and bioactivity of abscisic acid under UV radiation.

    Science.gov (United States)

    Gao, Fei; Hu, Tanglu; Tan, Weiming; Yu, Chunxin; Li, Zhaohu; Zhang, Lizhen; Duan, Liusheng

    2016-05-01

    Photosensitivity causes serious drawback for abscisic acid (ABA) application, but preferable methods to stabilize the compound were not found yet. To select an efficient photoprotectant for the improvement of photostability and bioactivity of ABA when exposed to UV light, we tested the effects of a photostabilizer bis(2,2,6,6-tetramethyl-4-piperidinyl) sebacate (HS-770) and two UV absorbers 2-hydroxy-4-n-octoxy-benzophenone (UV-531) and 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid (BP-4) with or without HS-770 on the photodegradation of ABA. Water soluble UV absorber BP-4 and oil soluble UV absorber UV-531 showed significant photo-stabilizing capability on ABA, possibly due to competitive energy absorption of UVB by the UV absorbers. The two absorbers showed no significant difference. Photostabilizer HS-770 accelerated the photodegradation of ABA and did not improve the photo-stabilizing capability of BP-4, likely due to no absorption in UVB region and salt formation with ABA and BP-4. Approximately 26% more ABA was kept when 280mg/l ABA aqueous solution was irradiated by UV light for 2h in the presence of 200mg/l BP-4. What's more, its left bioactivity on wheat seed (JIMAI 22) germination was greatly kept by BP-4, comparing to that of ABA alone. The 300 times diluent of 280mg/l ABA plus 200mg/l BP-4 after 2h irradiation showed more than 13% inhibition on shoot and root growth of wheat seed than that of ABA diluent alone. We concluded that water soluble UV absorber BP-4 was an efficient agent to keep ABA activity under UV radiation. The results could be used to produce photostable products of ABA compound or other water soluble agrichemicals which are sensitive to UV radiation. The frequencies and amounts of the agrichemicals application could be thereafter reduced. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Beneficial effects of solar UV-B radiation on soybean yield mediated by reduced insect herbivory under field conditions.

    Science.gov (United States)

    Mazza, Carlos A; Giménez, Patricia I; Kantolic, Adriana G; Ballaré, Carlos L

    2013-03-01

    Ultraviolet-B radiation (UV-B: 280-315 nm) has damaging effects on cellular components and macromolecules. In plants, natural levels of UV-B can reduce leaf area expansion and growth, which can lead to reduced productivity and yield. UV-B can also have important effects on herbivorous insects. Owing to the successful implementation of the Montreal Protocol, current models predict that clear-sky levels of UV-B radiation will decline during this century in response to ozone recovery. However, because of climate change and changes in land use practices, future trends in UV doses are difficult to predict. In the experiments reported here, we used an exclusion approach to study the effects of solar UV-B radiation on soybean crops, which are extensively grown in many areas of the world that may be affected by future variations in UV-B radiation. In a first experiment, performed under normal management practices (which included chemical pest control), we found that natural levels of UV-B radiation reduced soybean yield. In a second experiment, where no pesticides were applied, we found that solar UV-B significantly reduced insect herbivory and, surprisingly, caused a concomitant increase in crop yield. Our data support the idea that UV-B effects on agroecosystems are the result of complex interactions involving multiple trophic levels. A better understanding of the mechanisms that mediate the anti-herbivore effect of UV-B radiation may be used to design crop varieties with improved adaptation to the cropping systems that are likely to prevail in the coming decades in response to agricultural intensification. Copyright © Physiologia Plantarum 2012.

  2. Effect of UV-B radiation on biomass production, pigmentation and protein content of marine diatoms

    International Nuclear Information System (INIS)

    Doehler, G.

    1984-01-01

    Several species of marine diatoms were grown at + 18 0 C and + 22 0 C under normal air conditions (0.035 vol.% CO 2 ) at a light/dark alteration of 14.8 h. Intensity of white light was 1 mW (approx.= 5000 lux). An artifical nutrient solution of 35per mille salinity was used. Algae - harvested during exponential growth - were exposed to different intensities of UV-B radiation (439, 717 and 1230 J m -2 m -1 ) for 2 days. UV-B radiation depressed the growth of all tested marine diatoms. Low levels of UV-B resulted in a slight increase of the biomass production (dry weight) compared to not UV-B treated cells. Enhanced UV-B doses caused a diminution of the primary productivity in all species. Algae exposed to UV-B stress showed a marked decrease in the protein and pigment content (chlorophyll a, chlorophyll c 1 + c 2 and carotenoids). In + 22 0 C grown cells of Lauderia annulata and Thalassiosira rotula were more sensitive to UV-B radiation than those cultures grown at + 18 0 C. Bellerochea yucatanensis cells grown at + 22 0 C were less affected after UV-B exposure than at + 18 0 C grown algae. The UV-B sensibility and growth of the individual species varied in a mixture of several marine diatoms. Results were discussed with reference to the UV-B effect on metabolic processes. (orig.)

  3. "PROCESS and UVolution: photochemistry experiments in Low Earth Orbit": investigation of the photostability of organic and mineral material exposed to Mars surface UV radiation conditions

    Science.gov (United States)

    Stalport, Fabien; Guan, Yuan Yong; Noblet, Audrey; Coll, Patrice; Szopa, Cyril; Macari, Frederique; Person, Alain; Chaput, Didier; Raulin, Francois; Cottin, Hervé

    The harsh martian environment could explain the lack of organics and minerals such as car-bonates by destroying them: i) no organic molecule has been found at the two different landing sites of the Viking landers within the detection limits of the instruments onboard, ii) to date, no large deposits of carbonates have been detected and their detection is specific of local ar-eas and in very low amounts. In this context several experimental and numerical modelling studies were led to evaluate the possibility for the destruction or evolution of the organics and carbonates under the martian surface environmental conditions. The presence of UV radiation has been proposed to explain the photodecomposition of such material. This is the reason why, to investigate the nature, abundance, and stability of organic and mineral material that could survive under such environmental conditions, we exposed in low Earth orbit organic molecules and carbonates (also biominerals) with martian relevance to solar UV radiation ¿ 200 nm, in the frame of the experiment UVolution, onboard the BIOPAN ESA module which was set outside a Russian Foton automated capsule and exposed to space condition during 12 days in September 2007, and the experiment PROCESS (hervé peux tu rajouter quelques infos sur le temps exact d'exposition stp) which was set outside the International Space Station (ISS). Here, we present results with regard to the impact of solar UV radiation on the targeted molecules. Preliminary results indicate that that no organic sample seems to resist to the solar UV radiation if directly exposed to it. Conversely our results show that the exposed carbonates seem to be stable to the solar UV radiation if directly exposed to it. Moreover, the stability of the biominerals strengthens the interest to explore deeper their potential as life records at Mars. Hence they should be considered as primary targets for in situ analyses during future missions.

  4. Environmental radiation data, 9

    International Nuclear Information System (INIS)

    Nagaoka, Toshi; Sakamoto, Ryuichi; Saito, Kimiaki; Tsutsumi, Masahiro; Moriuchi, Shigeru

    1993-06-01

    The Environmental Physics Laboratory has conducted a large number of background radiation surveys in these years, aiming at the analysis of characteristics and behaviour of environmental radiation, the development of measurement techniques and instruments of environmental radiation, and the evaluation of environmental radiation dose. As the environmental radiation data obtained by these surveys are useful for broad purposes as actual survey data, it is desirable to arrange these data systematically and to open them to the other scientist. For that, it is necessary to make the recording media and the FORMAT of these data available for usual computers. In the light of this circumstance, these data were rearranged and recompiled systematically to meet the demand. This report mentions about the data obtained by the background radiation surveys in and around Tokyo performed during 1991 - 1993 using portable instruments, as well as the information necessary for the data handling. (author)

  5. Effects of ultraviolet-B radiation (UV-B) on growth and physiology of the dune grassland species Calamagrostis epigeios

    International Nuclear Information System (INIS)

    Tosserams, M.; Rozema, J.

    1995-01-01

    Seedlings of Calamagrostis epigeios were exposed to four levels of UV-B radiation (280-320 nm), simulating up to 44% reduction of stratospheric ozone concentration during summertime in The Netherlands, to determine the response of this plant species to UV-B irradiation. After six weeks of UV-B treatment, total biomass of all UV-B treated plants was higher, compared to plants that had received no UV-B radiation. The increase of biomass did not appear to be the result of a stimulation of net photosynthesis. Also, transpiration rate and water use efficiency were not altered by UV-B at any exposure level. Pigment analysis of leaf extracts showed no effect of enhanced UV-B radiation on chlorophyll content and accumulation of UV absorbing pigments. UV-B irradiance, however, did reduce the transmittance of visible light (400-700 nm) of intact attached leaves, suggesting a change in anatomical characteristics of the leaves. Additionally, the importance of including an ambient UV-B treatment in indoor experiments is discussed

  6. Effect of UV-B radiation on UV absorbing compounds and pigments of moss and lichen of Schirmacher oasis region, East Antarctica.

    Science.gov (United States)

    Singh, J; Gautam, S; Bhushan Pant, A

    2012-12-22

    The survival of Antarctic flora under ozone depletion depends on their ability to acclimate against increasing UV—B radiation by employing photo protective mechanisms either by avoiding or repairing UV—B damage. A fifteen days experiment was designed to study moss (Bryum argenteum) and lichen (Umbilicaria aprina) under natural UV—B exposure and under UV filter frames at the Maitri region of Schirmacher oasis, East Antarctica. Changes in UV absorbing compounds, phenolics, carotenoids and chlorophyll content were studied for continuous fifteen days and significant changes were observed in the UV exposed plants of B. argenteum and U. aprina. The change in the UV absorbing compounds was more significant in B. argenteum (P<0.0001) than U. aprina (P<0.0002). The change in phenolic contents and total carotenoid content was significant (P<0.0001) in both B. argenteum and lichen U. aprina indicating that the increase in UV absorbing compounds, phenolic contents and total carotenoid content act as a protective mechanism against the deleterious effect of UV—B radiations.

  7. Effects of UV-B radiation and water stress on gas exchange of soybeans under two different nitrogen levels

    International Nuclear Information System (INIS)

    Rosa, L.M.; Forseth, I.N.

    1993-01-01

    Due to anthropogenic destruction of stratospheric ozone, UV-B radiation is projected to increase in the near future. Other potential global climate changes in temperature and precipitation patterns raise the need for research into plant responses to multiple environmental stresses. The objective of this study was to document UV-B and water stress effects on gas exchange of soybean (Glycine max Merr.) under two nitrogen levels. Two soybean cultivars differing in sensitivity to UV-B were tested at fluence rates of 19.1 or 8.5 kJ m -2 day -1 (enhance and natural levels of UV-B, respectively). Measurements of photosaturated CO 2 uptake at ambient CO 2 (A). stomatal conductance. photosaturated O 2 evolution at saturating CO 2 (A max ), long term water use efficiency (using δ 13 C), and nitrogen fixation (using 15 N) were performed. No significant treatment effects on A could be detected. However A max was significantly increased, and stomatal conductance reduced (p<0.01) by increased UV-B at all levels of water and nitrogen for both cultivars, suggesting a stronger stomal limitation of photosynthesis under UV-B. Water and nitrogen use efficiency also decreased under increased UV-B in both cultivars (p<0.01)

  8. Photomorphogenic effects of UV-B radiation on plants: consequences for light competition

    International Nuclear Information System (INIS)

    Barnes, P.W.; Ballaré, C.L.; Caldwell, M.M.

    1996-01-01

    A combination of field and labotatory studies were conducted to explore the nature of photomorphogenic effects of ultraviolet-B radiation (UV-B; 280–320 nm) on plant morphology and to evaluate the ecological consequences of these alterations in morphology for interspecific competition. Under laboratory conditions, seedlings of cucumber (Cucumis sativus L.) and tomato (Lycopersicon esculentum Mill.) exhibited appreciable (ca. 50%) and rapid (< 3h) inhibition in hypocotyl elongation in response to UV-B exposure. In cucumber, this inhibition was reversible, occurred without any associated changes in dry matter production and was caused by UV-B incident on the cotyledons and not the stem or growing tip. Inhibition of stem elongation in etiolated tomato seedlings occurred at least 3 h prior to the onset of accumulation of UV-absorbing pigments and monochromatic UV supplied against a background of visible radiation revealed maximum effectiveness in inhibition around 300 nm. Collectively, these findings suggest that a specific, but yet unidentified, UV-B photoreceptor is involved in mediating certain morphological responses to UV-B. For mixtures of wheat (Triticum aestivum L.) and wild oat (Avena fatua L.), a common weedy competitor, supplemental UV-B irradiation in the field differentially altered shoot morphology which resulted in changes in canopy structure, light interception and calculated stand photosynthesis. It is argued that, because of its asymmetrical nature, competition for light can potentially amplify the effects of UV-B on shoot morphology and may, therefore, be an important mechanism by which changes in the solar UV-B spectrum associated with stratospheric ozone reduction could alter the composition and character of terrestrial vegetation

  9. The chemistry of UV and EB radiation curing

    International Nuclear Information System (INIS)

    Garnett, J.L.

    1987-01-01

    The application of photopolymerisation (UV) and electron beam (EB) technologies in radiation rapid cure (RRC) processing is discussed. The chemistry associated with such reactions and the mechanisms of the processes are treated. The occurrence of concurrent grafting to substrate with radiation curing of film is shown to be an advantage in enhancing the properties of certain finished products. The parameters influencing the optimum grafting yield in such RRC processes are discussed. In many applications, the chemistry of the process combined with the machine, expecially for EB, is shown a so-called ''turn-key'' operation. (author)

  10. The chemistry of UV and BE radiation curing

    International Nuclear Information System (INIS)

    Garnett, J.L.

    1991-01-01

    The application of photopolymerisation (UV) and electron beams (EB) technologies in radiation rapid cure (PRC) processing is discussed. The chemistry associated with such reactions and the mechanisms of the processes are treated. The occurrence of concurrent grafting to substrate with radiation curing of films is shown to be an advantage in enhancing the properties of certain finished products. The parameters influencing the optimum grafting yield in such PRC processes are discussed. In many applications, the chemistry of such processes combined with the machine, specially for EB is shown. (author)

  11. Octylphenol and UV-B radiation alter larval development and hypothalamic gene expression in the leopard frog (Rana pipiens).

    Science.gov (United States)

    Crump, Douglas; Lean, David; Trudeau, Vance L

    2002-03-01

    We assessed octylphenol (OP), an estrogenic endocrine-disrupting chemical, and UV-B radiation, a known stressor in amphibian development, for their effects on hypothalamic gene expression and premetamorphic development in the leopard frog Rana pipiens. Newly hatched tadpoles were exposed for 10 days to OP alone at two different dose levels; to subambient UV-B radiation alone; and to two combinations of OP and UV-B. Control animals were exposed to ethanol vehicle (0.01%) exposure, a subset of tadpoles from each treatment group was raised to metamorphosis to assess differences in body weight and time required for hindlimb emergence. Tadpoles from one of the OP/UV-B combination groups had greater body weight and earlier hindlimb emergence (p weight or hindlimb emergence, indicating a potential mechanism of interaction between OP and UV-B. We hypothesized that the developing hypothalamus might be a potential environmental sensor for neurotoxicologic studies because of its role in the endocrine control of metamorphosis. We used a differential display strategy to identify candidate genes differentially expressed in the hypothalamic region of the exposed tadpoles. Homology cloning was performed to obtain R. pipiens glutamate decarboxylases--GAD65 and GAD67, enzymes involved in the synthesis of the neurotransmitter gamma-aminobutyric acid (GABA). cDNA expression profiles revealed that OP and UV-B affected the levels of several candidate transcripts in tadpole (i.e., Nck, Ash, and phospholipase C gamma-binding protein 4 and brain angiogenesis inhibitor-3) and metamorph (i.e., GAD67, cytochrome C oxidase, and brain angiogenesis inhibitor-2 and -3) brains. This study represents a novel approach in toxicology that combines physiologic and molecular end points and indicates that levels of OP commonly found in the environment and subambient levels of UV-B alter the expression of important hypothalamic genes and disrupt tadpole growth patterns.

  12. Elevated UV-B radiation increased the decomposition of Cinnamomum camphora and Cyclobalanopsis glauca leaf litter in subtropical China

    Energy Technology Data Exchange (ETDEWEB)

    Song, Xinzhang Z.; Zhang, Huiling L.; Jiang, Hong; Yu, Shuquan Q. [Zhejiang Agriculture and Forestry Univ., Lin' an (China). The Nurturing Station for the State Key Lab. of Subtropical Silviculture; Zhejiang Agriculture and Forestry Univ., Lin' an (China). Zhejiang Provincial Key Lab. of Carbon Cycling and Carbon Sequestration in Forest Ecosystems; Chang, Scott X. [Alberta Univ., Edmonton (Canada). Dept. of Renewable Resources; Peng, Changhui H. [Quebec Univ., Montreal (Canada). Inst. of Environment Sciences

    2012-03-15

    Ultraviolet-B (UV-B) radiation reaching the earth's surface has been increasing due to ozone depletion and can profoundly influence litter decomposition and nutrient cycling in terrestrial ecosystems. The role of UV-B radiation in litter decomposition in humid environments is poorly understood; we thus investigated the effect of UV-B radiation on litter decomposition and nitrogen (N) release in a humid subtropical ecosystem in China. We conducted a field-based experiment using the litterbag method to study litter decomposition and N release under ambient and elevated (31% above ambient) UV-B radiation, using the leaf litter of two common tree species, Cinnamomum camphora and Cyclobalanopsis glauca, native to subtropical China. Elevated UV-B radiation significantly increased the decomposition rate of C. camphora and C. glauca leaf litter by 16.7% and 27.8%, respectively, and increased the N release from the decomposing litter of C. glauca but not C. camphora. Elevated UV-B radiation significantly accelerated the decomposition of litter of two native tree species and the N release from the decomposition litter of C. glauca in humid subtropical China, which has implications for soil carbon flux and forest productivity. (orig.)

  13. Solar UV-B radiation modulates chemical defenses against Anticarsia gemmatalis larvae in leaves of field-grown soybean.

    Science.gov (United States)

    Dillon, Francisco M; Chludil, Hugo D; Zavala, Jorge A

    2017-09-01

    Although it is well known that solar ultraviolet B (UV-B) radiation enhances plant defenses, there is less knowledge about traits that define insect resistance in field-grown soybean. Here we study the effects of solar UV-B radiation on: a) the induction of phenolic compounds and trypsin proteinase inhibitors (TPI) in soybean undamaged leaves or damaged by Anticarsia gemmatalis neonates during six days, and b) the survival and mass gain of A. gemmatalis larvae that fed on soybean foliage. Two soybean cultivars (cv.), Charata and Williams, were grown under plastic with different transmittance to solar UV-B radiation, which generated two treatments: ambient UV-B (UVB+) and reduced UV-B (UVB-) radiation. Solar UV-B radiation decreased survivorship by 30% and mass gain by 45% of larvae that fed on cv. Charata, but no effect was found in those larvae that fed on cv. Williams. TPI activity and malonyl genistin were induced by A. gemmatalis damage in both cultivars, but solar UV-B radiation and damage only synergistically increased the induction of these compounds in cv. Williams. Although TPI activity and genistein derivatives were induced by herbivory, these results did not explain the differences found in survivorship and mass gain of larvae that fed on cv. Charata. However, we found a positive association between lower larval performance and the presence of two quercetin triglycosides and a kaempferol triglycoside in foliage of cv. Charata, which were identified by HPLC-DAD/MS 2 . We conclude that exclusion of solar UV-B radiation reduce resistance to A. gemmatalis, due to a reduction in flavonol concentration in a cultivar that has low levels of genistein derivatives like cv. Charata. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Heated water and UV-C radiation to post harvest control of Cryptosporiopsis perennans on apples

    International Nuclear Information System (INIS)

    Bartnicki, Vinicius Adao; Amarante, Cassandro Vidal Talamini do; Castro, Luis Antonio Suita de; Rizzatti, Mara Regina; Souza, Joao Antonio Vargas de

    2010-01-01

    The objective of this work was to assess the colonization of Cryptosporiopsis perennans in the epidermis of apples and the efficiency of heated water and UV-C radiation application to control this pathogen. In apples inoculated with C. perennans, the colonization of lenticels and adjacent areas by the pathogen was observed by electronic scanning microscopy. The sensitivity of C. perennans conidia was evaluated in aqueous suspension, at temperatures of 28, 45, 50 and 55 deg C for 15 and 30 s, and at UV.C radiation doses of 0.018, 0.037, 0.075, 0.150, 0.375, 0.750, 1.500 and 3.000 kJ m.2. The effects of UV.C radiation doses at 0.375, 0.750 and 1.500 kJ m.2 and heated water at 50 deg C, sprayed during 15 and 30 s were evaluated for controlling C. perennans in apples inoculated with the pathogen. The fungus produced abundant mycelium and conidia in lenticels and adjacent areas on the epidermis of the apples. The heated water at 50 deg C during 15 s and a 0.750 kJ m.2 UV.C radiation dose reduced conidia survival in more than 99%. Heated water sprayed at 50 deg C during 15 s and a UV.C radiation dose of 0.375 kJ m.2 control C. perennans in apples. (author)

  15. Room-temperature effects of UV radiation in KBr:Eu{sup 2+} crystals

    Energy Technology Data Exchange (ETDEWEB)

    Perez-Salas, R; Melendrez, R [Centro de Investigacion Cientifica y de Educacion Superior de Ensenada - IFUNAM, Ensenada, Apartado Postal 2732 Ensenada, BC, 22800 (Mexico); Aceves, R; Rodriguez, R; Barboza-Flores, M [Centro de Investigacion en Fisica, Universidad de Sonora, Apartado Postal 5-088 Hermosillo, Sonora, 83190 (Mexico)

    1996-07-01

    Thermoluminescence and optical absorption measurements have been carried out in KBr:Eu{sup 2+} crystals irradiated with monochromatic UV light (200-300 nm) and x-rays at room temperature. For UV- and x-irradiated crystals strong similarities between the thermoluminescence glow curves have been found, suggesting that the low-energy UV radiation produces the same defects as produced by x-irradiation in this material. The thermoluminescence glow curves are composed of six glow peaks located at 337, 383, 403, 435, 475 and 509 K. Thermal annealing experiments in previously irradiated crystals show clearly a correlation between the glow peak located at 383 K and the F-centre thermal bleaching process. Also, the excitation spectrum for each thermoluminescence glow peak has been investigated, showing that the low-energy radiation induces the formation of F centres. (author)

  16. Effects of UV-B radiation on tetraspores of Chondrus ocellatus Holm (Rhodophyta), and effects of red and blue light on repair of UV-B-induced damage

    Science.gov (United States)

    Ju, Qing; Xiao, Hui; Wang, You; Tang, Xuexi

    2015-05-01

    We evaluated the effects of red and blue light on the repair of UV-B radiation-induced damage in tetraspores of Chondrus ocellatus Holm. Tetraspores of C. ocellatus were treated with different UV-B radiation levels (0, 36, 72, 108, 144 and 180 J/m2), and thereafter subjected to PAR, darkness, or red or blue light during a 2-h repair stage, each day for 48 days. The diameters and cellular contents of cyclobutane pyrimidine dimmers (CPDs), chlorophyll a (Chl a), phycoerythrin, and UV-B-absorbing mycosporinelike amino acids (MAAs) contents of the tetraspores were determined. Our results show that low doses of UV-B radiation (36 and 72 J/m2) promoted the growth of C. ocellatus; however, increased UV-B radiation gradually reduced the C. ocellatus growth (greater than 72 J/m2). The MAAs (palythine and asterina-330) in C. ocellatus were detected and analyzed by LC/MS. Our results suggest that moderate red light could induce the growth of this alga in aquaculture. In addition, photorepair was inhibited by red light, so there may be some other DNA repair mechanism activated by red light. Blue light promoted the activity of DNA photolyase, greatly improving remediation efficiency. Red and blue lights were found to reduce the capacity of C. ocellatus to form MAAs. Therefore, PAR, red light, and blue light play different roles during the repair processes for damage induced by UV-B radiation.

  17. Distinct physiological and metabolic reprogramming by highbush blueberry (Vaccinium corymbosum) cultivars revealed during long-term UV-B radiation.

    Science.gov (United States)

    Luengo Escobar, Ana; Alberdi, Miren; Acevedo, Patricio; Machado, Mariana; Nunes-Nesi, Adriano; Inostroza-Blancheteau, Claudio; Reyes-Díaz, Marjorie

    2017-05-01

    Despite the Montreal protocol and the eventual recovery of the ozone layer over Antarctica, there are still concerns about increased levels of ultraviolet-B (UV-B) radiation in the Southern Hemisphere. UV-B induces physiological, biochemical and morphological stress responses in plants, which are species-specific and different even for closely related cultivars. In woody plant species, understanding of long-term mechanisms to cope with UV-B-induced stress is limited. Therefore, a greenhouse UV-B daily course simulation was performed for 21 days with two blueberry cultivars (Legacy and Bluegold) under UV-B BE irradiance doses of 0, 0.07 and 0.19 W m -2 . Morphological changes, photosynthetic performance, antioxidants, lipid peroxidation and metabolic features were evaluated. We found that both cultivars behaved differently under UV-B exposure, with Legacy being a UV-B-resistant cultivar. Interestingly, Legacy used a combined strategy: initially, in the first week of exposure its photoprotective compounds increased, coping with the intake of UV-B radiation (avoidance strategy), and then, increasing its antioxidant capacity. These strategies proved to be UV-B radiation dose dependent. The avoidance strategy is triggered early under high UV-B radiation in Legacy. Moreover, the rapid metabolic reprogramming capacity of this cultivar, in contrast to Bluegold, seems to be the most relevant contribution to its UV-B stress-coping strategy. © 2016 Scandinavian Plant Physiology Society.

  18. Beta-Carotene production enhancement by UV-A radiation in Dunaliella bardawil cultivated in laboratory reactors

    International Nuclear Information System (INIS)

    Mogedas, B.; Casal, C.; Forjan, E.; Vilchez, C.

    2009-01-01

    beta-Carotene is an antioxidant molecule of commercial value that can be naturally produced by certain microalgae that mostly belong to the genus Dunaliella. So far, nitrogen starvation has been the most efficient condition for enhancing beta-carotene accumulation in Dunaliella. However, while nitrogen starvation promotes beta-carotene accumulation, the cells become non-viable; consequently under such conditions, continuous beta-carotene production is limited to less than 1 week. In this study, the use of UV-A radiation as a tool to enhance long-term beta-carotene production in Dunaliella bardawil cultures was investigated. The effect of UV-A radiation (320-400 nm) added to photosynthetically active radiation (PAR, 400-700 nm) on growth and carotenoid accumulation of D. bardawil in a laboratory air-fluidized bed photobioreactor was studied. The results were compared with those from D. bardawil control cultures incubated with PAR only. The addition of 8.7 W/square m UV-A radiation to 250 W/square m PAR stimulated long-term growth of D. bardawil. Throughout the exponential growth period the UV-A irradiated cultures showed enhanced carotenoid accumulation, mostly as beta-carotene. After 24 days, the concentration of beta-carotene in UV-A irradiated cultures was approximately two times that of control cultures. Analysis revealed that UV-A clearly induced major accumulation of all-trans beta-carotene. In N-starved culture media, beta-carotene biosynthesis in UV-A irradiated cultures was stimulated. We conclude that the addition of UV-A to PAR enhances carotenoid production processes, specifically all-trans beta-carotene, in D. bardawil cells without negative effects on cell growth

  19. Biological dosimetry to determine the UV radiation climate inside the MIR station and its role in vitamin D biosynthesis

    Science.gov (United States)

    Rettberg, P.; Horneck, G.; Zittermann, A.; Heer, M.

    1998-11-01

    The vitamin D synthesis in the human skin, is absolutely dependent on UVB radiation. Natural UVB from sunlight is normally absent in the closed environment of a space station like MIR. Therefore it was necessary to investigate the UV radiation climate inside the station resulting from different lamps as well as from occasional solar irradiation behind a UV-transparent quartz window. Biofilms, biologically weighting and integrating UV dosimeters successfully applied on Earth (e.g. in Antarctica) and in space (D-2, Biopan I) were used to determine the biological effectiveness of the UV radiation climate at different locations in the space station. Biofilms were also used to determine the personal UV dose of an individual cosmonaut. These UV data were correlated with the concentration of vitamin D in the cosmonaut's blood and the dietary vitamin D intake. The results showed that the UV radiation climate inside the Mir station is not sufficient for an adequate supply of vitamin D, which should therefore be secured either by vitamin D supplementat and/or by the regular exposure to special UV lamps like those in sun-beds. The use of natural solar UV radiation through the quartz window for `sunbathing' is dangerous and should be avoided even for short exposure periods.

  20. Differential responses of tetrasporophytes and gametophytes of Mazzaella laminarioides (Gigartinales, Rhodophyta) under solar UV radiation.

    Science.gov (United States)

    Navarro, Nelso P; Figueroa, Félix L; Korbee, Nathalie; Mansilla, Andrés; Plastino, Estela M

    2016-06-01

    The effects of solar UV radiation on mycosporine-like amino acids (MAAs), growth, photosynthetic pigments (Chl a, phycobiliproteins), soluble proteins (SP), and C and N content of Mazzaella laminarioides tetrasporophytes and gametophytes were investigated. Apical segments of tetrasporophytes and gametophytes were exposed to solar radiation under three treatments (PAR [P], PAR+UVA [PA], and PAR+UVA+UVB [PAB]) during 18 d in spring 2009, Punta Arenas, Chile. Samples were taken after 2, 6, 12, and 18 d of solar radiation exposure. Most of the parameters assessed on M. laminarioides were significantly influenced by the radiation treatment, and both gametophytes and tetrasporophytes seemed to respond differently when exposed to high UV radiation. The two main effects promoted by UV radiation were: (i) higher synthesis of MAAs in gametophytes than tetrasporophytes at 2 d, and (ii) a decrease in phycoerythrin, phycocyanin, and SPs, but an increase in MAA content in tetrasporophytes at 6 and 12 d of culture. Despite some changes that were observed in biochemical parameters in both tetrasporophytes and gametophytes of M. laminarioides when exposed to UVB radiation, these changes did not promote deleterious effects that might interfere with the growth in the long term (18 d). The tolerance and resistance of M. laminarioides to higher UV irradiance were expected, as this intertidal species is exposed to variation in solar radiation, especially during low tide. © 2016 Phycological Society of America.

  1. Formulation comprising silicon microparticles, as a pigment that can absorb visible UV radiation and reflect ir radiation

    OpenAIRE

    Rodríguez, Marie-Isabelle; Fenollosa Esteve, Roberto; Meseguer, Francisco

    2011-01-01

    [EN] The invention relates to a formulation characterised in that it comprises silicon microparticles having a size between 0.010 um and 50 um in diameter, and to the use thereof as a pigment that can absorb visible UV radiation and reflect IR radiation.

  2. Polycyclic aromatic hydrocarbons (PAHs) skin permeation rates change with simultaneous exposures to solar ultraviolet radiation (UV-S).

    Science.gov (United States)

    Hopf, Nancy B; Spring, Philipp; Hirt-Burri, Nathalie; Jimenez, Silvia; Sutter, Benjamin; Vernez, David; Berthet, Aurelie

    2018-05-01

    Road construction workers are simultaneously exposed to two carcinogens; solar ultraviolet (UV-S) radiation and polycyclic aromatic hydrocarbons (PAHs) in bitumen emissions. The combined exposure may lead to photogenotoxicity and enhanced PAH skin permeation rates. Skin permeation rates (J) for selected PAHs in a mixture (PAH-mix) or in bitumen fume condensate (BFC) with and without UV-S co-exposures were measured with in vitro flow-through diffusion cells mounted with human viable skin and results compared. Possible biomarkers were explored. Js were greater with UV-S for naphthalene, anthracene, and pyrene in BFC (0.08-0.1 ng/cm 2 /h) compared to without (0.02-0.26 ng/cm 2 /h). This was true for anthracene, pyrene, and chrysene in the PAH-mix. Naphthalene and benzo(a)pyrene (BaP) in the PAH-mix had greater Js without (0.97-13.01 ng/cm 2 /h) compared to with UV-S (0.40-6.35 ng/cm 2 /h). Time until permeation (T lags ) in the PAH-mix were generally shorter compared to the BFC, and they ranged from 1 to 13 h. The vehicle matrix could potentially be the reason for this discrepancy as BFC contains additional not identified substances. Qualitative interpretation of p53 suggested a dose-response with UV-S, and somewhat with the co-exposures. MMP1, p65 and cKIT were not exploitable. Although not statistically different, PAHs permeate human viable skin faster with simultaneous exposures to UV. Copyright © 2018 Elsevier B.V. All rights reserved.

  3. Radiation chemical oxidation of propen under the influence of UV- and gamma radiation

    International Nuclear Information System (INIS)

    Litschke, P.I.

    1978-01-01

    The oxidation of propen is studied in the liquid state under the influence of electromagnetic radiation using hydrogenperoxide, organic hydroperoxides and oxygen. In this investigation propen oxide is of main interest. The study of systems with oxygen is based on the concept that the formation of hydroperoxide from organic oxygen compounds is enhanced by irradiation, thus favouring an in situ method for expoxidation with hydroperoxides. The influence of UV-radiation from high and low pressure mercury discharge lamps and 60 Co gamma radiation has been studied as well as the effect of solvents and catalysers, which are resolved in the system. (orig./WBU) [de

  4. Survival of thermophilic and hyper-thermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation

    International Nuclear Information System (INIS)

    Beblo, K.; Wirth, R.; Huber, H.; Douki, T.; Schmalz, G.; Rachel, R.

    2011-01-01

    In this study, we investigated the ability of several (hyper-) thermophilic Archaea and phylo-genetically deep-branching thermophilic Bacteria to survive high fluences of monochromatic UV-C (254 nm) and high doses of ionizing radiation, respectively. Nine out of fourteen tested microorganisms showed a surprisingly high tolerance against ionizing radiation, and two species (Aquifex pyrophilus and Ignicoccus hospitalis) were even able to survive 20 kGy. Therefore, these species had a comparable survivability after exposure to ionizing radiation such as Deinococcus radiodurans. In contrast, there was nearly no difference in survival of the tested strains after exposure to UV-C under anoxic conditions. If the cells had been dried in advance of UV-C irradiation, they were more sensitive to UV-C radiation compared with cells irradiated in liquid suspension; this effect could be reversed by the addition of protective material like sulfidic ores before irradiation. By exposure to UV-C, photoproducts were formed in the DNA of irradiated Archaea and Bacteria. The distribution of the main photoproducts was species specific, but the amount of the photoproducts was only partly dependent on the applied fluence. Overall, our results show that tolerance to radiation seems to be a common phenomenon among thermophilic and hyper-thermophilic microorganisms. (authors)

  5. Elevated UV-B radiation incident on Quercus robur leaf canopies enhances decomposition of resulting leaf litter in soil

    International Nuclear Information System (INIS)

    Newsham, K.K.; Greenslade, P.D.; Kennedy, V.H.; McLeod, A.R.

    1999-01-01

    We examined whether the exposure of Quercus robur L. to elevated UV-B radiation (280–315 nm) during growth would influence leaf decomposition rate through effects on litter quality. Saplings were exposed for eight months at an outdoor facility in the UK to a 30% elevation above the ambient level of erythemally weighted UV-B radiation under UV-B treatment arrays of fluorescent lamps filtered with cellulose diacetate, which transmitted both UV-B and UV-A (315–400 nm) radiation. Saplings were exposed to elevated UV-A alone under control arrays of lamps filtered with polyester and to ambient radiation under unenergised arrays of lamps. Abscised leaves from saplings were enclosed in 1 mm2 mesh nylon bags, placed in a Quercus–Fraxinus woodland and were sampled at 0.11, 0.53, 1.10 and 1.33 years for dry weight loss, chemical composition and saprotrophic fungal colonization. At abscission, litters from UV-A control arrays had ≈ 7.5% higher lignin/nitrogen ratios than those from UV-B treatment and ambient arrays (P < 0.06). Dry weight loss of leaves treated with elevated UV-B radiation during growth was 2.5% and 5% greater than that of leaves from UV-A control arrays at 0.53 and 1.33 years, respectively. Litter samples from UV-B treatment arrays lost more nitrogen and phosphorus than samples from ambient arrays and more carbon than samples from UV-A control arrays. The annual fractional weight loss of litter from UV-B treatment arrays was 8% and 6% greater than that of litter from UV-A control and ambient arrays, respectively. Regression analyses indicated that the increased decomposition rate of UV-B treated litters was associated with enhanced colonization of leaves by basidiomycete fungi, the most active members of the soil fungal community, and that the frequency of these fungi was negatively associated with the initial lignin/nitrogen ratio of leaves. (author)

  6. Decontamination of poultry feed from ochratoxin A by UV and sunlight radiations.

    Science.gov (United States)

    Ameer Sumbal, Gul; Hussain Shar, Zahid; Hussain Sherazi, Syed Tufail; Sirajuddin; Nizamani, Shafi Muhammad; Mahesar, Safaraz Ahmed

    2016-06-01

    Mycotoxin-contaminated feed is very dangerous for the growth and even life of poultry. The objective of the current study was to investigate the efficacy of ultra-violet irradiation for decontamination of ochratoxin A (OTA) in spiked and naturally contaminated poultry feed samples. Spiked and naturally contaminated feed samples were irradiated with ultra-violet light (UV) at distance of 25 cm over the feed samples. In vitro, the effect of UV intensity (0.1 mW cm(-2) at 254 nm UV-C) on different types of poultry feeds contaminated with OTA was evaluated. The same samples were also irradiated with sunlight and analysed for OTA by an indirect enzyme linked immunosorbent assay method. Poultry feed samples containing 500 µg kg(-1) were 100% decontaminated in 180 min with UV radiation while OTA was decreased to 70-95 µg kg(-1) using the same poultry feed samples after 8 h sunlight irradiation. Therefore, UV light was found to be more effective. Only 1 h of UV irradiation was found to be sufficient to bring the OTA level to the maximum regulatory limit suggested for poultry feeds (100 µg kg(-1) ), while 8 h were needed to obtain this level using sunlight radiations. The proposed approach is a viable option to reduce the level of OTA in contaminated poultry feeds. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  7. [Effects of silicon supply on diurnal variations of physiological properties at rice heading stage under elevated UV-B radiation].

    Science.gov (United States)

    Wu, Lei; Lou, Yun-sheng; Meng, Yan; Wang, Wei-qing; Cui, He-yang

    2015-01-01

    A pot experiment was conducted to investigate the effects of silicon (Si) supply on diurnal variations of photosynthesis and transpiration-related physiological parameters at rice heading stage under elevated UV-B radiation. The experiment was designed with two UV-B radiation levels, i.e. ambient UV-B. (ambient, A) and elevated UV-B (elevated by 20%, E), and four Si supply levels, i.e. Sio (control, 0 kg SiO2 . hm-2), Si, (sodium silicate, 100 kg SiO2 . hm-2), Si2 (sodium silicate, 200 kg SiO2 . hm2), Si3 (slag fertilizer, 200 kg SiO2 . hm-2). The results showed that, compared with ambient UV-B radiation, elevated UV-B radiation decreased the net photosynthesis rate (Pn) , intercellular CO2 concentration (Ci), transpiration rate (Tr), stomatal conductivity (gs) and water use efficiency (WUE) by 11.3%, 5.5%, 10.4%, 20.3% and 6.3%, respectively, in the treatment without Si supply (Si, level), and decreased the above parameters by 3.8%-5.5%, 0.7%-4.8%, 4.0%-8.7%, 7.4%-20.2% and 0.7%-5.9% in the treatments with Si supply (Si1, Si2 and Si3 levels) , respectively. Namely, elevated UV-B radiation decreased the photosynthesis and transpiration-related physiological parameters, but silicon supply could obviously mitigate the depressive effects of elevated UV-B radiation. Under elevated UV-B radiation, compared with control (Si0 level), silicon supply increased Pn, Ci, gs and WUE by 16.9%-28.0%, 3.5%-14.3%, 16.8% - 38.7% and 29.0% - 51.2%, respectively, but decreased Tr by 1.9% - 10.8% in the treatments with Si supply (Si1 , Si2 and Si3 levels). That is, silicon supply could mitigate the depressive effects of elevated UV-B radiation through significantly increasingnP., CigsgK and WUE, but decreasing T,. However, the difference existed in ameliorating the depressive effects of elevated UV-B radiation on diurnal variations of physiological parameters among the treatments of silicon supply, with the sequence of Si3>Si2>1i >Si0. This study suggested that fertilizing slag was

  8. Effects of different levels of UV-B radiation on marine epilithic communities: a short-term microcosm study

    Directory of Open Access Journals (Sweden)

    R. A. Danilov

    2000-12-01

    Full Text Available Epilithic communities from brackish coastal water of the east coast of Sweden were studied in order to reveal their responses to different levels of UV-B radiation. The experiment was conducted for seven days in an indoor microcosm system. The epilithic communities were exposed daily to different doses of UV-B radiation: 1, 3, 5 and 7 hours, respectively. The intensity of the UV-B radiation used was similar to that in natural environments. After seven days clear shifts both in species composition and community structure were observed. All species of diatoms showed negative correlation with enhanced levels of UV-B. On the other hand, abundance of cyanobacteria was positively correlated with enhanced levels of UV-B (Calothrix aeruginea, R=0.49;Oscillatoria amphibia, R=0.93; Pseudanabaena mucicola, R=0.26. Communities dominated by diatoms and green algae at the initial stage of the experiment did show a drastic decrease in diatom abundance under UV-B stress and finally became dominated by cyanobacteria. Among green algae the abundance of Cladophora sericea correlated positively (R=0.33 with enhanced exposure to UV-B, while Monoraphidium-species exhibited negative correlation to UV-B. Otherwise, no significant correlation between other green algae species identified and UV-B stress could be detected. We conclude that UV-B radiation, even at lower levels, is capable to induce severe changes to marine epilithic communities. The trends revealed in the present study predict a shift from communities dominated by diatoms and green algae to those dominated by cyanobacteria.

  9. Effect of heat, UV radiation, and moisture on the decohesion kinetics of inverted organic solar cells

    KAUST Repository

    Rolston, Nicholas

    2017-06-15

    Organic solar cells subjected to environmental stressors such as heat, moisture, and UV radiation can undergo significant mechanical degradation, leading to delamination of layers and device failure. This paper reports the effect these stressors have on the mechanical integrity of active layers and interfaces as measured by subcritical debonding tests, and the in situ evolution of defects and fracture processes is characterized. At elevated temperatures below 50 °C in inert conditions, significant device weakening was observed, an effect we attributed to a temperature-induced P3HT:PCBM delamination mechanism from the underlying ZnO. At 50 °C in ambient conditions with UV exposure—selected to better simulate real-world environments—devices were more resistant to fracture because of an interfacial strengthening effect from increased hydrogen bonding where UV-induced Zn(OH)2 formation reinforced the interface with P3HT:PCBM. This photoinduced hydroxylation mechanism was determined from a decrease in the Zn/O ratio with increased UVA or UVB exposure, and hydroxylation was shown to directly correlate with the resistance to fracture in devices.

  10. In vitro study of cytotoxicity by U.V. radiation and differential sensitivity in combination with alkylating agents on established cell systems

    International Nuclear Information System (INIS)

    Ramudu, K.

    1991-01-01

    The effect of U.V. radiation or alkylating agents, such as actinomycin-D, cycloheximide and mitomycin-C (MMC), was studied on CHO, BHK and HeLa cells. U.V. radiation caused DNA ssb and dsb and were prevented by cycloheximide and actinomycin-D. MMC is known to be cytotoxic in CHO/BHK cells by forming free radical generation. MMC in combination with U.V. radiation enhanced DNA ssb ampersand dsb in these cell types. However, HeLa cells were insensitive to U.V. radiation. This insensitivity to U.V. radiation could be ascribed to the presence of glutathione transferase which is absent in CHO/BHK cell line

  11. UV light induced DNA damages and the radiation protection effects of Lingzi mushroom extract

    International Nuclear Information System (INIS)

    Vo Thi Thuong Lan; Dinh Ba Tuan; Ta Bich Thuan; Tran Bang Diep; Tran Minh Quynh

    2016-01-01

    UV light has strongly influenced on the growth of E. coli as well as caused DNA damages. Configurations of both genomic DNA and pUC 19 plasmids extracted from E. coli were significantly changed by the exposure to UV light of 254 nm and DLT, an extract of Ganoderma lucidum Lingzi mushroom. The results also revealed the radio-protective effects of DLT to UV radiation. By adding 2% DLT to its culturing suspension, the growth of E. coli was significantly decreased, whereas a low DLT amount of about 0.5% slightly improved its growth, indicated that the DLT extract can be used as a promising protective substance against UV radiation. At the molecular level, the radio-protective effects of DLT were observed for both UV treated DNA and protein. Thus, DLT can protect DNA in vivo, but not in vitro. This effect was also observed for Taq polymerase, suggested that the radioprotection effect of DLT may due to it accelerated the degradation of radicals or species that produced in the suspensions during UV exposure. (author)

  12. Radiation damage and repair in cells and cell components. Progress report, November 1, 1977--October 31, 1978. [Uv and x radiation, bacteriophages

    Energy Technology Data Exchange (ETDEWEB)

    Fluke, D.J.; Pollard, E.C.

    1978-01-01

    Progress is reported on the following research projects: coordinate induction of mutagenesis, radioresistance, and inhibition of post-radiation DNA degradation; radioinduced filamentation; action spectrum for induction of K12 lambda phage; effects of uv radiation on cells in the frozen state; dependence of mutagenesis on wavelength of uv; and w-reactivation of x-irradiated phage lambda. (HLW)

  13. Preparation of inorganic crystalline compounds induced by ionizing, UV and laser radiation

    International Nuclear Information System (INIS)

    Cuba, V.; Pavelkova, T.; Barta, J.; Indrei, J.; Gbur, T.; Pospisil, M.; Mucka, V.; Docekalova, Z.; Zavadilova, A.; Vlk, M.

    2011-01-01

    Complete text of publication follows. Radiation methods represent powerful tool for synthesis of various inorganic materials. Study of solid particles formation from solutions in the field of UV or ionizing radiation is one of the very promising and long term pursued trends in photochemistry and radiation chemistry. The motivation may be various, either preparation of new materials or removal of hazardous contaminants (e.g. heavy metals) from wastewater. This work deals with preparation of some metal oxides, synthetic garnets and spinel structures via irradiation of aqueous solutions containing precursors, i.e. soluble metal salts, radical scavengers and/or macromolecular stabilizers. Namely, results on radiation induced preparation of nickel, zinc, yttrium and aluminium oxides are summarized, as well as zinc peroxide, yttrium / lutetium - aluminium garnets and cobalt(II) aluminate. 60 Co irradiator, linear electron accelerator, medium pressure UV lamp and solid state laser were used as the sources of radiation. Aside from preparation, various physico-chemical and structural properties of compounds prepared were also studied. All used modifications of radiation method are rather convenient and simple, and yield (nano)powder materials with interesting characteristics. Prepared materials generally have high chemical purity, high specific surface area and narrow distribution of particles size (ranging in tens of nm). Generally, all types of irradiation result in materials with comparable properties and structural characteristics; but in the case of synthetic garnets and spinels, preparation using UV-radiation seems to be the most convenient for their preparation. Among compounds discussed, only zinc oxide and zinc peroxide were prepared directly via irradiation. For preparation of other crystalline compounds, additional heat treatment (at low temperature) of amorphous solid phase formed under irradiation was necessary.

  14. The UV Sensor Onboard the Mars Science Laboratory Mission: Correction and Generation of UV Fluxes

    Science.gov (United States)

    Vicente-Retortillo, Á.; Martinez, G.; Renno, N. O.; Lemmon, M. T.; Gomez-Elvira, J.

    2017-12-01

    The Rover Environmental Monitoring Station UV sensor (UVS) onboard the Mars Science Laboratory mission has completed more than 1750 sols of measurements, providing an unprecedented coverage ranging from diurnal to interannual times scales [1,2]. The UVS is comprised of six photodiodes to measure the UV flux in the ranges 200-380, 320-380, 280-320, 200-280, 230-290 and 300-350 nm [3]. UV fluxes in units of W/m2 can be found in the NASA Planetary Data System (PDS). However, dust deposition on the UVS and a non-physical discontinuity in the calibration functions when the solar zenith angle is above 30º cause errors in these fluxes that increase with time. We have developed a technique to correct UV fluxes from the effects of dust degradation and inconsistencies in the angular response of the UVS. The photodiode output currents (available in the PDS as lower-level TELRDR products), ancillary data records (available in the PDS as ADR products) and dust opacity values derived from Mastcam observations are used for performing the corrections. The corrections have been applied to the UVA band (320-380 nm) for the first 1000 sols of the mission, providing excellent results [4]. We plan to correct the UV fluxes on each of the six UVS bands and to make these results available in the PDS. Data products generated by this study will allow comparisons of the UV radiation environment at Gale crater with that at the locations of the future missions ExoMars 2020 and Mars 2020, as well as the assessment of the potential survivability of biological contaminants brought to Mars from Earth. References: [1] Smith, M. D., et al. (2016), Aerosol optical depth as observed by the Mars Science Laboratory REMS UV photodiodes, Icarus, 280, 234-248. [2] Vicente-Retortillo, Á., et al. (2017), Determination of dust aerosol particle size at Gale Crater using REMS UVS and Mastcam measurements, Geophys. Res. Lett., 44, 3502-3508. [3] Gómez-Elvira, J., et al. (2012), REMS: The environmental sensor

  15. Influence of light, UV-B radiation, and herbicides on wax biosynthesis of cucumber seedlings

    International Nuclear Information System (INIS)

    Tevini, M.; Steinmüller, D.

    1987-01-01

    The behavior of cuticular alkane-1-ols and alkanes were studied in different developmental stages of cucumber seedlings grown in the dark or under white light, with or without UV-B radiation or in presence of wax biosynthesis inhibitors, trichloroacetic acid and metolachlor. Accumulation of alkane-1-ols increased light independently with seedling age. Synthesis of alkanes was strictly light and dose dependent. Addition of UV-B radiation did not alter the amounts of alkanes or alcohols, however, the distribution of homologues was shifted towards shorter chain homologues. Treatments with Cl 3 AcOH resulted in strong inhibition of alkane accumulation, whereas the amount of alkane-1-ols was changed neither at low nor at moderate concentrations of Cl 3 AcOH but their homologue distribution shifted towards longer chain lengths. This shifting was depressed in the presence of UV-B. At high concentrations of Cl 3 Ac0H similar homologue distributions as produced by UV-B (shift to shorter homologues) were observed. Metolachlor treatment resulted in an inhibition of alkane-1-ol production connected with rising amounts of alkanes, predominantly of short chain species. A simple model of wax biosynthesis is proposed which describes the interactions with white light, UV-B radiation and herbicides. (author)

  16. Effect of UV-C radiation on bioactive compounds of pineapple (Ananas comosus L. Merr.) by-products.

    Science.gov (United States)

    Freitas, Ana; Moldão-Martins, Margarida; Costa, Helena S; Albuquerque, Tânia G; Valente, Ana; Sanches-Silva, Ana

    2015-01-01

    The industrial processing of pineapple generates a high quantity of by-products. To reduce the environmental impact of these by-products and the inherent cost of their treatment, it is important to characterise and valorise these products, converting them into high added value products. Ultra-violet radiation is one of the main sustainable sanitation techniques for fruits. Since this radiation can induce plant stress which can promote the biosynthesis of bioactive compounds, it is important to evaluate its effect in fruits. The amounts of vitamins (C and E) and carotenoids (α-carotene, β-carotene, β-cryptoxanthin, lutein, lycopene, neoxanthin, violaxanthin and zeaxanthin) in pineapple by-products (core and rind) were analysed before and after treatment with UV radiation. All treated and untreated pineapple by-products contained β-carotene as the main carotenoid (rind, 2537-3225 µg; and core, 960-994 µg 100 g(-1) DW). Pineapple rind also contained lutein (288-297 µg 100 g(-1) DW) and α-carotene (89-126 µg 100 g(-1) DW). The results provide evidence of the potential of pineapple by-products as a source of bioactive compounds with antioxidant activity, which can be used by pharmaceutical, cosmetics and food industries. In addition, UV-C was shown to be a treatment that can add nutritional value to pineapple by-products. © 2014 Society of Chemical Industry.

  17. Impact of UV-B radiation on photosynthetic assimilation of 14C-bicarbonate and inorganic 15N-compounds by cyanobacteria

    International Nuclear Information System (INIS)

    Doehler, G.; Biermann, I.; Zink, J.

    1986-01-01

    The cyanobacteria Anabaena cylindrica and Synechococcus leopoliensis (=Anacystis nidulans) were grown at different levels of UV-B radiation (439, 717, 1230 and 1405 J m -2 d -1 , weighted according Caldwell, 1971) for 2 days. Dry weight was hardly affected but phycocyanin content of both species decreased linearly to the level of UV-B radiation. Contents of protein, carotenoids and chlorophyll a were reduced only after exposure to high doses (1230 J m -2 d -1 ) of UV-B radiation. Photosynthetic 14 CO 2 fixation of Anabaena cells was reduced linearly with increasing UV-B dose whereas no effect could be observed in Synechococcus. A depression of photosynthetic 15 N-nitrate uptake was found after UV-B stress in both species. UV-B irradiance caused an increase of 15 N-incorporation into glutamine, but no effect was noted for incorporation into alanine or aspartic acid. An increase of 15 N-excess in glutamic acid linear with the UV-B dose was observed in Synechococcus, only. Patterns of 14 C-labelled photosynthetic products were either less affected by UV-B radiation (Anabaena) or an enhancement of 14 C-label in total amino acids was detected (Synechococcus). The amount of total free amino acids increased parallel to the level of UV-B radiation. Only, the high dose of UV-B (1405 J m -2 d -1 , weighted) results in a decrease of the glutamine pool. Our results indicate an inhibition of glutamate synthase by UV-B irradiation in Anabaena, only. Results were discussed with reference to the damage of the photosynthetic apparatus. (orig.)

  18. Action spectrum and mechanisms of UV radiation-induced injury in lupus erythematosus

    International Nuclear Information System (INIS)

    Kochevar, I.E.

    1985-01-01

    Photosensitivity associated with lupus erythematosus (LE) is well established. The photobiologic basis for this abnormal response to ultraviolet radiation, however, has not been determined. This paper summarizes the criteria for elucidating possible photobiologic mechanisms and reviews the literature relevant to the mechanism of photosensitivity in LE. In patients with LE, photosensitivity to wavelengths shorter than 320 nm has been demonstrated; wavelengths longer than 320 nm have not been adequately evaluated. DNA is a possible chromophore for photosensitivity below 320 nm. UV irradiation of skin produces thymine photodimers in DNA. UV-irradiated DNA is more antigenic than native DNA and the antigenicity of UV-irradiated DNA has been proposed, but not proven, to be involved in the development of clinical lesions. UV irradiation of mice previously injected with anti-UV-DNA antibodies produces Ig deposition and complement fixation that appears to be similar to the changes seen in lupus lesions. Antibodies to UV-irradiated DNA occur in the serum of LE patients although a correlation between antibody titers and photosensitivity was not observed. Defective repair of UV-induced DNA damage does not appear to be a mechanism for the photosensitivity in LE. Other mechanisms must also be considered. The chromophore for photosensitivity induced by wavelengths longer than 320 nm has not been investigated in vivo. In vitro studies indicate that 360-400 nm radiation activates a photosensitizing compound in the lymphocytes and serum of LE patients and causes chromosomal aberrations and cell death. The mechanism appears to involve superoxide anion

  19. The effects of different UV-B radiation intensities on morphological and biochemical characteristics in Ocimum basilicum L.

    Science.gov (United States)

    Sakalauskaitė, Jurga; Viskelis, Pranas; Dambrauskienė, Edita; Sakalauskienė, Sandra; Samuolienė, Giedrė; Brazaitytė, Aušra; Duchovskis, Pavelas; Urbonavičienė, Dalia

    2013-04-01

    The effects of short-term ultraviolet B (UV-B) irradiation on sweet basil (Ocimum basilicum L. cv. Cinnamon) plants at the 3-4 leaf pair and flowering stages were examined in controlled environment growth chambers. Plants were exposed to 0 (reference), 2 and 4 kJ UV-B m(-2) day(-1) over 7 days. Exposure of basil plants to supplementary UV-B light resulted in increased assimilating leaf area, fresh biomass and dry biomass. Stimulation of physiological functions in young basil plants under either applied UV-B dose resulted in increased total chlorophyll content but no marked variation in carotenoid content. At the flowering stage the chlorophyll and carotenoid contents of basil were affected by supplementary UV-B radiation, decreasing with enhanced UV-B exposure. Both total antioxidant activity (2,2-diphenyl-1-picrylhydrazyl free radical assay) and total phenolic compound content were increased by UV-B light supplementation. Young and mature basil plants differed in their ascorbic acid content, which was dependent on UV-B dose and plant age. UV-B radiation resulted in decreased nitrate content in young basil plants (3-4 leaf pair stage). These results indicate that the application of short-exposure UV-B radiation beneficially influenced both growth parameters and biochemical constituents in young and mature basil plants. © 2012 Society of Chemical Industry.

  20. Effects of Ultraviolet (UV) Radiations at Different Wave Lengths on ...

    African Journals Online (AJOL)

    Prof. Ogunji

    The effects of UV-radiation on the bacterial load and yeast viability of palm wine were ... shelf life due to the uncontrolled metabolic activities of yeast and bacteria. .... Process. Biochemistry International 8:23-220. Okafor, N. (2007). Palm Wine ...

  1. Ambient solar UV radiation and seasonal trends in potential sunburn ...

    African Journals Online (AJOL)

    Background. The detrimental effects of excess personal solar ultraviolet (UV) radiation exposure include sunburn, immunosuppression and skin cancer. In South Africa, individuals with minimum natural protection from melanin, including fair-skinned individuals and African albinos, and people spending extended ...

  2. Optical Protection Filters for Harmful Laser Beams and UV Radiation

    Science.gov (United States)

    Azim M., Osama A.

    2007-02-01

    Due to the rapid growth of radiation protection applications in various devices and instruments, it is essential to use suitable filters for eye protection of the personal working in the radiation field. Different protection filters were produced to protect from four laser beam wavelengths (at 532nm, 632.8nm, 694nm and 1064nm) and block three UV bands (UVA, UVB, and UVC). The design structure of the required dielectric multilayer filters used optical thin film technology. The computer analyses of the multilayer filter formulas were prepared using Macleod Software for the production filter processes. The deposition technique was achieved on optical substrates (Glass BK-7 and Infrasil 301) by dielectric material combinations including Dralo (mixture of oxides TiO2/Al2O3), and Lima (mixture of oxides SiO2/Al2O3); deposition by an electron beam gun. The output transmittance curves for both theoretical and experimental values of all filters are presented. To validate the suitability for use in a `real world', rather than laboratory test application, full environmental assessment was also carried out. These filters exhibited high endurance after exposing them to the durability tests (adhesion, abrasion resistance and humidity) according to military standards MIL-C-675C and MIL-C-48497A.

  3. Optical Protection Filters for Harmful Laser Beams and UV Radiation

    International Nuclear Information System (INIS)

    Azim M, Osama A.

    2007-01-01

    Due to the rapid growth of radiation protection applications in various devices and instruments, it is essential to use suitable filters for eye protection of the personal working in the radiation field. Different protection filters were produced to protect from four laser beam wavelengths (at 532nm, 632.8nm, 694nm and 1064nm) and block three UV bands (UVA, UVB, and UVC). The design structure of the required dielectric multilayer filters used optical thin film technology. The computer analyses of the multilayer filter formulas were prepared using Macleod Software for the production filter processes. The deposition technique was achieved on optical substrates (Glass BK-7 and Infrasil 301) by dielectric material combinations including Dralo (mixture of oxides TiO2/Al2O3), and Lima (mixture of oxides SiO2/Al2O3); deposition by an electron beam gun. The output transmittance curves for both theoretical and experimental values of all filters are presented. To validate the suitability for use in a 'real world', rather than laboratory test application, full environmental assessment was also carried out. These filters exhibited high endurance after exposing them to the durability tests (adhesion, abrasion resistance and humidity) according to military standards MIL-C-675C and MIL-C-48497A

  4. The Skin Microbiome: Is It Affected by UV-induced Immune Suppression?

    OpenAIRE

    Patra, VijayKumar; Byrne, Scott N.; Wolf, Peter

    2016-01-01

    Human skin apart from functioning as a physical barricade to stop the entry of pathogens, also hosts innumerable commensal organisms. The skin cells and the immune system constantly interact with microbes, to maintain cutaneous homeostasis, despite the challenges offered by various environmental factors. A major environmental factor affecting the skin is ultraviolet radiation (UV-R) from sunlight. UV-R is well known to modulate the immune system, which can be both beneficial and deleterious. ...

  5. THE ACTION OF UV RADIATION ON MITOTIC INDEX AND MITOTIC DIVISION PHASES AT PHASEOLUS VULGARIS L

    Directory of Open Access Journals (Sweden)

    Csilla Iuliana Bara

    2005-08-01

    Full Text Available In this work, damaging effects of UV radiations on bean Phaseolus vulgaris L. plantule root tips were investigated. Our study proves that by bean plants, the decrease of cell division frequency appears to be part of protection mechanism against especially the short waved UV radiation, with variations depending on cultivar.

  6. TiO2 nanoparticles as an effective UV-B radiation skin-protective compound in sunscreens

    International Nuclear Information System (INIS)

    Popov, A P; Priezzhev, A V; Lademann, J; Myllylae, R

    2005-01-01

    Protecting human skin against harmful UV-B radiation coming from the sun is currently a problem. Due to the decreased thickness of the ozone layer, a more dangerous amount of UV-B light reaches the surface of our planet. This causes increased frequency of skin diseases. Titanium dioxide (TiO 2 ) fine particles are embedded with sunscreens into the skin to effectively attenuate UV-B radiation. This study evaluates the most appropriate size of such particles assuming they are spheres. The distribution of TiO 2 particles within the skin, achieved with topically applied sunscreens, is determined experimentally by the tape-stripping technique. Computer code implementing the Monte Carlo method is used to simulate photon migration within the plain 20 μm thick horny layer matrix partially filled with nano-sized TiO 2 particles. Dependences of harmful UV-B radiation of 307-311 nm absorbed by, backscattered from and transmitted through the horny layer on the concentration of TiO 2 particles are obtained and analysed. As a result, particles of 62 nm are found to be the most effective in protecting skin against UV-B light

  7. [Effects of silicon supply on rice growth and methane emission from paddy soil under elevated UV-B radiation].

    Science.gov (United States)

    Meng, Yan; Lou, Yun-sheng; Wu, Lei; Cui, He-yang; Wang, Wei-qing

    2015-01-01

    A pot experiment was conducted to investigate the effects of silicon supply on rice growth and methane (CH4) emission in paddy field under elevated UV-B radiation. The experiment was designed with two UV-B radiation levels, i.e. ambient UV-B (ambient, A) and elevated UV-B radiation (elevated by 20%, E) ; with four silicon supply levels, i.e., Si0 (control, without silicon), Si2 (as sodium silicate, 100 kg SiO2 . hm-2), Si2 (as sodium silicate, 200 kg SiO2 hm-2) and Si3 (as slag fertilizer, 200 kg SiO2 . hm-2). The results indicated that, silicon supply obviously alleviated the depressive effect of elevated UV-B radiation on rice growth, and increased the tiller numbers, chlorophyll content, and shoot and root dry masses. Silicon supply promoted rice growth, which increased with the silicon supply level (sodium silicate). Slag fertilizer was better than*sodium silicate in promoting rice growth. CH4 flux and accumulated CH4emission were obviously increased by elevated UV-B radiation, but significantly decreased by silicon application. CH4 emission was reduced with increasing the silicon supply level. Under the same silicon supply level, slag fertilizer was better than sodium silicate in inhibiting CH4 flux and accumulated CH4 emission. This research suggested that fertilizing slag in rice production was helpful not only in utilizing industrial wastes, but also in significantly mitigating CH4 emissions in rice paddy under elevated UV-B radiation.

  8. Alteration of foliar flavonoid chemistry induced by enhanced UV-B radiation in field-grown Pinus ponderosa, Quercus rubra and Pseudotsuga menziesii.

    Science.gov (United States)

    Warren, Jeffrey M; Bassman, John H; Mattinson, D Scott; Fellman, John K; Edwards, Gerald E; Robberecht, Ronald

    2002-03-01

    Chromatographic analyses of foliage from several tree species illustrate the species-specific effects of UV-B radiation on both quantity and composition of foliar flavonoids. Pinus ponderosa, Quercus rubra and Pseudotsuga menziesii were field-grown under modulated ambient (1x) and enhanced (2x) biologically effective UV-B radiation. Foliage was harvested seasonally over a 3-year period, extracted, purified and the flavonoid fraction applied to a mu Bondapak/C(18) column HPLC system sampling at 254 nm. Total flavonoid concentrations in Quercus rubra foliage were more than twice (leaf area basis) that of the other species; Pseudotsuga menziesii foliage had intermediate levels and P. ponderosa had the lowest concentrations of total flavonoids. No statistically significant UV-B radiation-induced effects were found in total foliar flavonoid concentrations for any species; however, concentrations of specific compounds within each species exhibited significant treatment effects. Higher (but statistically insignificant) levels of flavonoids were induced by UV-B irradiation in 1- and 2-year-old P. ponderosa foliage. Total flavonoid concentrations in 2-year-old needles increased by 50% (1x ambient UV-B radiation) or 70% (2x ambient UV-B radiation) from that of 1-year-old tissue. Foliar flavonoids of Q. rubra under enhanced UV-B radiation tended to shift from early-eluting compounds to less polar flavonoids eluting later. There were no clear patterns of UV-B radiation effects on 1-year-old P. menziesii foliage. However, 2-year-old tissue had slightly higher foliar flavonoids under the 2x UV-B radiation treatment compared to ambient levels. Results suggest that enhanced UV-B radiation will alter foliar flavonoid composition and concentrations in forest tree species, which could impact tissue protection, and ultimately, competition, herbivory or litter decomposition.

  9. Color, physicochemical parameters and antioxidant potential of whole grape juices subject to different UV-C radiation doses

    Directory of Open Access Journals (Sweden)

    Paôla de Castro Henrique

    2016-04-01

    Full Text Available ABSTRACT Knowing that moderate stress such as UV radiation can activate defense mechanisms in plants, the use of UV-C radiation appears as hypothesis of a promising technique that would help to stimulate and enhance beneficial compounds for health, through a clean and healthy technology. In this study, the possible induction of secondary metabolism, the increase in the content of phytochemical compounds and physicochemical changes through the use of UV-C radiation were evaluated on whole grape juices produced with Vitis labrusca grapes, cultivar Isabel Precoce. Grapes were harvested, sanitized, exposed to UV-C radiation at doses of 0, 2, 4 and 6 KJ m-2, and then the juices were prepared and packed into amber glass bottles at room temperature. Analyses were performed at 0, 30, 60, 90 and 120 days of storage. Based on results obtained and conditions in which the experiment was performed, UV-C treatment in grapes caused abiotic stress in the fruits, affecting color, titratable acidity, soluble solids/titratable acidity ratio, vitamin C and percentage of protection against oxidation. Application of UV-C did not change levels of phenolic compounds in fruit juices or the percentage of scavenging free radicals, pH and soluble solids.

  10. Exclusion of UV-B radiation from normal solar spectrum on the growth of mung bean and maize

    International Nuclear Information System (INIS)

    Pal, M.; Sharma, A.; Abrol, Y.P.; Sengupta, U.K.

    1997-01-01

    The increase in UV-B radiation due to depletion of the ozone layer has potentially harmful effects on plant growth and performance. The bulk of these studies conducted in growth chambers, greenhouses or in the field use different types of exposure systems which may be responsible for differences in the sensitivity of a crop to UV-B radiation. A field study using selective filters to remove the UV-B portion of the solar spectrum was conducted with mung bean (a dicotyledonous C 3 plant) and maize (a monocotyledonous C 4 plant) to determine the sensitivity of these crop plants to ambient UV-B levels without disturbing the microenvironment. Mung bean was found to be sensitive to ambient UV-B levels in terms of leaf area development, plant height attained and net photosynthesis, while maize was found to be unaffected by ambient UV-B levels (22.8 |GmW cm −2 nm −1 ) found in Delhi, India (28°38′N, 77°13′E). The level of ambient UV-B radiation thus appears to be inhibitory for optimal growth of plants, especially dicotyledonous mung bean. (author)

  11. Crosslink the Novel Group of Polymeric Binders BioCo by the UV-radiation

    Directory of Open Access Journals (Sweden)

    Grabowska B.

    2016-06-01

    Full Text Available The spectroscopic FT-IR and FT-Raman methods allowed to identify the cross-linking process of the aqueous composition of poly(acrylic acid/sodium salt of carboxymethyl starch (PAA/CMS-Na applied as a binder for moulding sands (as a novel group binders BioCo. The cross-linking was performed by physical agent, applying the UV-radiation. The results of structural studies (IR, Raman confirm the overlapping of the process of cross-linking polymer composition PAA/CMS-Na in UV radiation. Taking into account the ingredients and structure of the polymeric composition can also refer to a curing process in a binder - mineral matrix mixture. In the system of binder-mineral matrix under the influence of ultraviolet radiation is also observed effect of binding. However, the bonding process does not occur in the entire volume of the investigated system, but only on the surface, which gives some possibilities for application in the use of UV curing surface of cores, and also to cure sand moulds in 3D printing technology.

  12. Recent studies on UV radiation in Brazil

    Science.gov (United States)

    Correa, M. P.; Ceballos, J. C.; Moregula, A.; Okuno, E.; Fausto, A.; Mol, A.; Santos, J. C.

    2009-04-01

    This presentation shows a summary of UV index measurements performed in the last years in Southeastern (SE) and Northeastern (NE) Brazilian regions. Brazil has an area of 8.5 million km2 distributed between latitudes 5˚ N and 35˚ S and longitudes 5˚ W and 75˚ W. SE is the most important economic pole of South America and the NE coast is an important tourist region. This large area has a great diversity of climatic, atmospheric and geographical conditions in addition to very diverse social and cultural habits. Non-melanoma skin cancer (NMSC) is an epidemiological health problem with more than 120,000 new cases each year. The most of these cases are found in the South and Southeast regions, with about 70 new NMSC per 100,000 inhabitants. Solar Light UV501 biometers are installed in the SE cities of São Paulo (23.6˚ S, 46.7˚ W, 865 m ASL), Itajubá/Minas Gerais (22.4˚ S; 45.5˚ W, 846 m ASL) and the NE city of Ilhéus/Bahia (14.8˚ S; 39.3˚ W; 54 m ASL). First measurements began in 2005 in São Paulo city, while Itajubá and Ilhéus have regular measurements from the beginning of 2008. Other studies related to the UV radiation modeling and interactions with atmosphere components, as ozone, aerosols and clouds, have also been performed. For example: a) UVI modelling calculations performed by a multiple-scattering spectral models; b) studies on the aerosol radiative properties based on satellite (MODIS/Terra-Aqua) and ground-based (Aeronet) observation; c) ozone content variability from satellite (OMI/Aura) and ground-based (Microtops ozonometer) measurements; d) behavioral profile of the population, as regarding habits of solar exposure and sun protection measures. Results show that more than 75% of the measurements conducted in the summer (outside noon) can be classified as upper than high UVI according to World Health Organization (WHO) recommended categories: Low (UVI UV radiation levels to have a population very exposed during its

  13. Complex responses of intertidal molluscan embryos to a warming and acidifying ocean in the presence of UV radiation.

    Directory of Open Access Journals (Sweden)

    Andrew R Davis

    Full Text Available Climate change and ocean acidification will expose marine organisms to synchronous multiple stressors, with early life stages being potentially most vulnerable to changing environmental conditions. We simultaneously exposed encapsulated molluscan embryos to three abiotic stressors-acidified conditions, elevated temperate, and solar UV radiation in large outdoor water tables in a multifactorial design. Solar UV radiation was modified with plastic filters, while levels of the other factors reflected IPCC predictions for near-future change. We quantified mortality and the rate of embryonic development for a mid-shore littorinid, Bembicium nanum, and low-shore opisthobranch, Dolabrifera brazieri. Outcomes were consistent for these model species with embryos faring significantly better at 26°C than 22°C. Mortality sharply increased at the lowest temperature (22°C and lowest pH (7.6 examined, producing a significant interaction. Under these conditions mortality approached 100% for each species, representing a 2- to 4-fold increase in mortality relative to warm (26°C non-acidified conditions. Predictably, development was more rapid at the highest temperature but this again interacted with acidified conditions. Development was slowed under acidified conditions at the lowest temperature. The presence of UV radiation had minimal impact on the outcomes, only slowing development for the littorinid and not interacting with the other factors. Our findings suggest that a warming ocean, at least to a threshold, may compensate for the effects of decreasing pH for some species. It also appears that stressors will interact in complex and unpredictable ways in a changing climate.

  14. Complex Responses of Intertidal Molluscan Embryos to a Warming and Acidifying Ocean in the Presence of UV Radiation

    Science.gov (United States)

    Davis, Andrew R.; Coleman, Daniel; Broad, Allison; Byrne, Maria; Dworjanyn, Symon A.; Przeslawski, Rachel

    2013-01-01

    Climate change and ocean acidification will expose marine organisms to synchronous multiple stressors, with early life stages being potentially most vulnerable to changing environmental conditions. We simultaneously exposed encapsulated molluscan embryos to three abiotic stressors—acidified conditions, elevated temperate, and solar UV radiation in large outdoor water tables in a multifactorial design. Solar UV radiation was modified with plastic filters, while levels of the other factors reflected IPCC predictions for near-future change. We quantified mortality and the rate of embryonic development for a mid-shore littorinid, Bembicium nanum, and low-shore opisthobranch, Dolabrifera brazieri. Outcomes were consistent for these model species with embryos faring significantly better at 26°C than 22°C. Mortality sharply increased at the lowest temperature (22°C) and lowest pH (7.6) examined, producing a significant interaction. Under these conditions mortality approached 100% for each species, representing a 2- to 4-fold increase in mortality relative to warm (26°C) non-acidified conditions. Predictably, development was more rapid at the highest temperature but this again interacted with acidified conditions. Development was slowed under acidified conditions at the lowest temperature. The presence of UV radiation had minimal impact on the outcomes, only slowing development for the littorinid and not interacting with the other factors. Our findings suggest that a warming ocean, at least to a threshold, may compensate for the effects of decreasing pH for some species. It also appears that stressors will interact in complex and unpredictable ways in a changing climate. PMID:23405238

  15. Role of p-aminobenzoic acid in the repair of injuries induced by UV- and. gamma. -radiation

    Energy Technology Data Exchange (ETDEWEB)

    Rapoport, I A; Vasil' eva, S V; Davnichenko, L S [AN SSSR, Moscow. Inst. Khimicheskoj Fiziki

    1979-07-01

    For the first time it was proved that low doses of p-aminobenzoic acid (PABA) were capable of sharply decreasing lethal mutational effects of UV light and less significantly-gamma effect on a bacterial cell. The experiments were carried out on E.Coli strains which differed in the activity of ferment system of DNA UV-induced injuries reparation. UV radiation dose equaled 10-1500 erd/mm/sup 2/. PABA capability to intensify the reparative process under mutagenic effects of 3 main types: chemical, UV as a representative of non-penetrating radiation, and penetrating radiation permitted to characterize this compound as ''reparagen''. It was emphasized that the application of reparagens capable of intensifying or weakening the reparative process permitted to observe different effects of reparation dependence on the concentration of a chemical agent being introduced from outside and localize the process of reparagen effect in time.

  16. The influence of UV radiation on protistan evolution

    Science.gov (United States)

    Rothschild, L. J.

    1999-01-01

    Ultraviolet radiation has provided an evolutionary challenge to life on Earth. Recent increases in surficial ultraviolet B fluxes have focused attention on the role of UV radiation in protistan ecology, cancer, and DNA damage. Exploiting this new wealth of data, I examine the possibility that ultraviolet radiation may have played a significant role in the evolution of the first eukaryotes, that is, protists. Protists probably arose well before the formation of a significant ozone shield, and thus were probably subjected to substantial ultraviolet A, ultraviolet B, and ultraviolet C fluxes early in their evolution. Evolution consists of the generation of heritable variations and the subsequent selection of these variants. Ultraviolet radiation has played a role both as a mutagen and as a selective agent. In its role as a mutagen, it may have been crucial in the origin of sex and as a driver of molecular evolution. As a selective agent, its influence has been broad. Discussed in this paper are the influence of ultraviolet radiation on biogeography, photosynthesis, and desiccation resistance.

  17. In the shadow of the Cosmetic Directive — Inconsistencies in EU environmental hazard classification requirements for UV-filters

    International Nuclear Information System (INIS)

    Sobek, A.; Bejgarn, S.; Rudén, C.; Molander, L.; Breitholtz, M.

    2013-01-01

    UV-filters are chemicals with potentially environmental hazardous properties. In the European Union (EU), UV-filters contained in sunscreen products are currently regulated by the Cosmetic Directive (from July 2013 by the Cosmetic Products Regulation). Environmental hazard classifications according to the regulation on classification, labelling and packaging of substances and mixtures (CLP) must be determined for UV-filters contained in industrial chemical products, whereas UV-filters contained in sunscreens are exempted from CLP. In this study we determined the potential environmental hazard classifications of UV-filters and sunscreen products if the CLP regulation was to be required for cosmetic products. Two sunscreen products were evaluated in accordance with the aquatic environmental hazard criteria for mixtures. The results highlight that the inconsistencies in the current EU regulation of UV filters hamper the risk management of environmental hazards of UV filters used in cosmetic products. Almost 50% of the investigated UV-filters approved for use in cosmetic products on the European market according to the current Cosmetic Directive were identified to meet the CLP classification as being hazardous to the aquatic environment. Assuming a worst-case scenario, the two examined sunscreens could both be classified as hazardous to the aquatic environment with long-lasting effects according to CLP classification criteria. Hence, if the CLP regulation was applicable to sunscreen products, both brands could potentially be labelled with the environmental hazard pictogram and associated hazard and precautionary statements. Including cosmetic products, and thereby sunscreens, in the CLP regulation would contribute to a more harmonized and transparent regulation of potentially hazardous substances on the EU market. - Highlights: • UV-filters are used in both cosmetic and industrial products/applications • UV-filters in cosmetic products are excluded from CLP • We

  18. Environmental radiation sensing technologies

    International Nuclear Information System (INIS)

    Nishizawa, Hiroshi; Inomata, Kenji; Tamuro, Masaru; Fujita, Kazuhiko

    2013-01-01

    After the Fukushima nuclear accident, environmental radiation monitoring and radioactivity measurement of contamination of wastes, soils, food and drinking water were needed in accurate and reliable way. Based on radiation sensing technologies and radiation and light coupled analysis method, new environmental radiation measurement system for simple monitoring post without exclusive house and also portable monitoring post for temporary use were developed with low cost. Measurement accuracy was improved by real-time processing of detected pulses and corrected non-linearity of low-energy range by analysis. Environmental performance was upgraded to assure detector gain with compensated against temperature change and aging. Inspection and maintenance were also simplified using touch panel display with standardized application menu and data format. (T. Tanaka)

  19. Drinking water disinfection by means of ultraviolet radiation. Desinfektion von Trinkwasser durch UV-Bestrahlung

    Energy Technology Data Exchange (ETDEWEB)

    Gelzhaeuser, P; Bewig, F; Holm, K; Kryschi, R; Reich, G; Steuer, W

    1985-01-01

    The book presents all lectures held during a course at Technical Academy Esslingen, on September 10, 1985, on the subject of 'Drinking water disinfection by means of ultraviolet radiation'. The methods hitherto used for disinfection are no longer suitable because of the increasing amounts of organic pollutants found in the untreated water, and because of the necessity to make drinking water disinfection less expensive, non-polluting and thus environmentally compatible. U.V. irradiation is a method allowing technically simple and safe disinfection of the water, and also does not have any effect on the natural taste of the drinking water. The lectures presented discuss all aspects of the method, the equipment, and the performance of irradiation systems in practice.

  20. Toxic effects of combined effects of anthracene and UV radiation on Brachionus plicatilis

    Science.gov (United States)

    Gao, Ceng; Zhang, Xinxin; Xu, Ningning; Tang, Xuexi

    2017-05-01

    Anthracene is a typical polycyclic aromatic hydrocarbon, with photo activity, can absorb ultraviolet light a series of chemical reactions, aquatic organisms in the ecosystem has a potential light induced toxicity. In this paper, the effects of anthracene and UV radiation on the light-induced toxicity of Brachionus plicatilis were studied. The main methods and experimental results were as follows: (1) The semi-lethal concentration of anthracene in UV light was much lower than that in normal light, The rotifers have significant light-induced acute toxicity. (2) Under UV irradiation, anthracene could induce the increase of ROS and MDA content in B. plicatilis, and the activity of antioxidant enzymes in B. plicatilis significantly changed, Where SOD, GPx activity was induced within 24 hours of the beginning of the experiment. And the content of GPX and CAT was inhibited after 48 hours. Therefore, the anthracite stress induced by UV radiation could more strongly interfere with the ant oxidative metabolism of B. plicatilis, and more seriously cause oxidative damage, significant light-induced toxicity.

  1. The formation of ozone and UV radiation from high-power pulsed electric discharges

    Science.gov (United States)

    Piskarev, I. M.; Ushkanov, V. A.; Selemir, V. D.; Spirov, G. M.; Malevannaya Pikar', I. A.; Zuimach, E. A.

    2008-09-01

    High-power electric discharges with pulse energies of from 0.15 J to 4 kJ were studied. The yields of UV photons and ozone were found to be approximately equal, which led us to conclude that discharge conditions under which UV radiation and ozone fully destroyed each other were possible. If ozone formation was suppressed, as when a negative volume charge was created in the spark gap region, the flux of UV photons reached 3 × 1023 photons/(cm2 s).

  2. Long-term variability and impact on human health of biologically active UV radiation in Moscow

    Science.gov (United States)

    Zhdanova, Ekaterina; Chubarova, Natalia

    2014-05-01

    Measurements of erythemally weighted UV irradiance (Qer) have been performed at the Meteorological Observatory of Moscow State University since 1999 with the UVB-1 YES pyranometers. These types of devices are broadband with a spectral sensitivity curve close to the action spectrum of erythema. Main uncertainties of UVB-1 YES measurements include the difference in spectral curves of the instrument and the action spectrum of erythema, as well as the deviation from the cosine law. These uncertainties were taken into account in the database of Qer measurements (Chubarova, 2008. Additional corrections of UVB-1 measurements at low ambient temperatures have been made. We analyze interannual, seasonal and diurnal Qer changes over the time period 1999-2012. In addition, the comparisons with the results of UV reconstruction model (Chubarova, 2008) are made. This model allows us to evaluate relative changes in Qer due to variations in total ozone, effective cloud amount transmission, aerosol and cloud optical thickness since 1968. It is important to note that the main reason for UV irradiance monitoring development is the strong influence of UV irradiance on the biosphere and especially on human health mainly on human skin (CIE, 1993, CIE, 2006) and eyes (Oriowo, M. et al., 2001). Based on the detailed studies we have shown the possibility of utilizing UVB-1 pyranometers for measuring the eye-damage UV radiation. Parallel measurements by the Bentham DTM-300 spectrometer and the UVB-1 YES pyranometer at the Innsbruck Medical University (Austria) have provided us the calibration factor in eye-damage units for this broadband instrument. Influence of main geophysical factors on different types of UV irradiance is estimated by means the RAF ideology (Booth, Madronich, 1994). We discuss the responses of different types of biologically active UV radiation to the impact of various atmospheric factors. The UV conditions (deficiency, optimum, excess for human) are analyzed according to

  3. Increased exposure to UV-B radiation during early development leads to enhanced photoprotection and improved long-term performance in Lactuca sativa.

    Science.gov (United States)

    Wargent, Jason J; Elfadly, Eslam M; Moore, Jason P; Paul, Nigel D

    2011-08-01

    Plant responses to solar UV radiation are numerous and have often been considered from a perspective of negative outcomes for plant productivity. In this study, we used two experimental approaches consisting of: (1) field-based spectrally modifying filters in addition to (2) controlled indoor exposure to UV-B, to examine the effects of UV radiation on growth and photosynthetic performance of lettuce (Lactuca sativa L.) seedlings. Various aspects of growth were affected in plants grown under a UV-inclusive environment compared to a UV-depleted environment, including reductions in leaf expansion, increases in leaf thickness and the rate of net photosynthesis. After transplantation to a uniform field environment, lettuce plants initially propagated under the UV-inclusive environment exhibited higher harvestable yields than those from a UV-depleted environment. In controlled conditions, photosynthetic rates were higher in plants grown in the presence of UV-B radiation, and relative growth of plants pre-acclimatized to UV-B was also increased, in addition to higher maximum photochemical efficiency of photosystem II (PSII) (F(v) /F(m) ) following subsequent exposure to high photosynthetically active radiation (PAR) and temperature stress. Our findings are discussed within the context of sustainability in agriculture and the paradigm shift in photobiology which such beneficial responses to UV radiation could represent. © 2011 Blackwell Publishing Ltd.

  4. Significance of grafting in radiation curing reactions. Comparison of ionising radiation and UV systems

    International Nuclear Information System (INIS)

    Zilic, E.; Ng, L.; Viengkhou, V.; Garnett, J.L.

    1998-01-01

    Full text: Radiation curing is now an accepted commercial technology where both ionising radiation (electron beam) and ultra violet light (UV) sources are used. Grafting is essentially the copolymerisation of a monomer/oligomer to a backbone polymer whereas curing is the rapid polymerisation of a monomer/oligomer mixture onto the surface of the substrate. There is no time scale theoretically associated with grafting processes which can occur in minutes or hours whereas curing reactions are usually very rapid, occurring within a fraction of a second. An important difference between grafting and curing is the nature of the bonding occurring in each process. In grafting covalent carbon-carbon bonds are formed, whereas in curing, bonding usually involves weaker Van der Waals or London dispersion forces. The bonding properties of the systems are important in determining their use commercially. Thus the possibility that concurrent grafting during curing could occur in a system is important since if present, grafting would not only minimise delamination of the coated product but could also, in some circumstances, render difficulties recycling of the finished product especially if it were cellulosic. Hence the conditions for observing the occurrence of concurrent grafting during radiation curing are important. In the present paper, this problem has been studied by examining the effect that the components used in radiation curing exert on a typical reaction. Instead of electron beam sources, the spent fuel element facility at Lucas Heights is used to simulate such ionising radiation sources. The model system utilised is the grafting of a typical methacrylate to cellulose. This is the generic chemistry used in curing systems. The effect of typical additives from curing systems including polyfunctional monomer and oligomers in the grafting reactions have been studied. The ionising radiation results have been compared with analogous data from UV experiments. The significance

  5. The effects of UV-B radiation on European heathland species

    DEFF Research Database (Denmark)

    Björn, Lars O.; Callaghan, T. V.; Johnsen, Ib

    1997-01-01

    in the ozone layer) with artificially increased precipitation. The Swedish experiment also involved a study of the interaction between enhanced UV-B radiation and elevated CO2 (600 ppm). These field studies were supported by an outdoor controlled environment study in the United Kingdom involving modulated...

  6. Dielectric spectroscopy of [P(NID2OD-T2)]n thin films: Effects of UV radiation on charge transport

    International Nuclear Information System (INIS)

    Sepulveda, Pablo I.; Rosado, Alexander O.; Pinto, Nicholas J.

    2014-01-01

    Poly[N,N′-bis(2-octyldodecyl)-naphthalene-1,4,5,8-bis(dicarboximide) -2,6-diyll-alt-5,5′-(2,2′-bithiophene)]-[P(ND12OD-T2)] n is a n-doped polymer that is stable in air. Low frequency (40 Hz–30 kHz) dielectric spectroscopy shows that the polymer impedance strength is reduced under ultra-violet (UV) radiation as a result of charge increase in the bulk polymer. Photo-excitation and the creation of electron-hole pairs and subsequent hole recombination with electron trapping species adsorbed by the polymer are suggested as possible doping mechanisms. The relaxation times were also faster in the presence of UV indicating multiple pathways for oscillating dipoles to relax. These results imply increased polymer conductance with corresponding enhancement of charge mobility due to reduced scattering in the presence of UV radiation. A thin film field effect transistor was fabricated using this polymer as the active material and characterized in the presence of UV radiation. As expected, the device exhibited n-type behavior with a charge mobility of 3.0 × 10 −3 cm 2 /V-s. Exposure to UV radiation increased the channel current, shifted the threshold voltage to more negative values and doubled the value of the mobility. These results are consistent with dielectric measurements and suggest an easy method of increasing device currents and charge mobility in this polymer via UV irradiation. - Highlights: • Ultra-violet (UV) radiation dopes the polymer. • The doping is n-type. • UV radiation enhances charge mobility without post polymer processing. • Dielectric spectroscopy and field effect transistor results are self-consistent

  7. Validity of satellite measurements used for the monitoring of UV radiation risk on health

    Directory of Open Access Journals (Sweden)

    F. Jégou

    2011-12-01

    Full Text Available In order to test the validity of ultraviolet index (UVI satellite products and UVI model simulations for general public information, intercomparison involving three satellite instruments (SCIAMACHY, OMI and GOME-2, the Chemistry and Transport Model, Modélisation de la Chimie Atmosphérique Grande Echelle (MOCAGE, and ground-based instruments was performed in 2008 and 2009. The intercomparison highlighted a systematic high bias of ~1 UVI in the OMI clear-sky products compared to the SCIAMACHY and TUV model clear-sky products. The OMI and GOME-2 all-sky products are close to the ground-based observations with a low 6 % positive bias, comparable to the results found during the satellite validation campaigns. This result shows that OMI and GOME-2 all-sky products are well appropriate to evaluate the UV-risk on health. The study has pointed out the difficulty to take into account either in the retrieval algorithms or in the models, the large spatial and temporal cloud modification effect on UV radiation. This factor is crucial to provide good quality UV information. OMI and GOME-2 show a realistic UV variability as a function of the cloud cover. Nevertheless these satellite products do not sufficiently take into account the radiation reflected by clouds. MOCAGE numerical forecasts show good results during periods with low cloud covers, but are actually not adequate for overcast conditions; this is why Météo-France currently uses human-expertised cloudiness (rather than direct outputs from Numerical Prediction Models together with MOCAGE clear-sky UV indices for its operational forecasts. From now on, the UV monitoring could be done using free satellite products (OMI, GOME-2 and operational forecast for general public by using modelling, as long as cloud forecasts and the parametrisation of the impact of cloudiness on UV radiation are adequate.

  8. Plant responses to UV-B irradiation are modified by UV-A irradiation

    International Nuclear Information System (INIS)

    Middleton, E.M.; Teramura, A.H.

    1993-01-01

    The increasing UV-B radiation (0.28-0.32 μm) reaching the earth's surface is an important concern. Plant response in artificial UV-B irradiation studies has been difficult to assess, especially regarding photosynthetic pigments, because the fluorescent lamps also produce UV-A (0.32-0.40μm) radiation which is involved with blue light in pigment synthesis. Both UV-A and UV-B irradiances were controlled in two glasshouse experiments conducted under relatively high PPFD (> 1300μmol m -2 s -1 ) at two biologically effective daily UV-B irradiances (10.7 and 14.1 kJ m -2 ); UV-A irradiances were matched in Controls (∼5, 9 kJ m -2 ). Normal, chlorophyll-deficient, and flavonoid-deficient isolines of soybean cultivar, Clark, were utilized. Many growth/ pigment variables exhibited a statistically significant interaction between light quality and quantity: in general, UV-A radiation moderated the damaging effects of UV-B radiation. Regression analyses demonstrated that a single negative function related photosynthetic efficiency to carotenoid Content (r 2 =0.73, P≤0.001), implying a open-quotes costclose quotes in maintaining carotenoids for photoprotection. A stomatal limitation to photosynthesis was verified and carotenoid content was correlated with UV-B absorbing compound levels, in UV-B irradiated plants

  9. UV-induced skin damage

    International Nuclear Information System (INIS)

    Ichihashi, M.; Ueda, M.; Budiyanto, A.; Bito, T.; Oka, M.; Fukunaga, M.; Tsuru, K.; Horikawa, T.

    2003-01-01

    Solar radiation induces acute and chronic reactions in human and animal skin. Chronic repeated exposures are the primary cause of benign and malignant skin tumors, including malignant melanoma. Among types of solar radiation, ultraviolet B (290-320 nm) radiation is highly mutagenic and carcinogenic in animal experiments compared to ultraviolet A (320-400 nm) radiation. Epidemiological studies suggest that solar UV radiation is responsible for skin tumor development via gene mutations and immunosuppression, and possibly for photoaging. In this review, recent understanding of DNA damage caused by direct UV radiation and by indirect stress via reactive oxygen species (ROS) and DNA repair mechanisms, particularly nucleotide excision repair of human cells, are discussed. In addition, mutations induced by solar UV radiation in p53, ras and patched genes of non-melanoma skin cancer cells, and the role of ROS as both a promoter in UV-carcinogenesis and an inducer of UV-apoptosis, are described based primarily on the findings reported during the last decade. Furthermore, the effect of UV on immunological reaction in the skin is discussed. Finally, possible prevention of UV-induced skin cancer by feeding or topical use of antioxidants, such as polyphenols, vitamin C, and vitamin E, is discussed

  10. Biological effects of N+ ion implantation and UV radiation on streptomyces albus

    International Nuclear Information System (INIS)

    Wu Jian; Dai Guifu

    2005-01-01

    The results of both 30 keV N + ion implantation and UV irradiation of Streptomyces albus showed complicate biological effects. The 'saddle shape' pattern of the dose-dependent curve formed by N + ion implantation with low energy was studied, and it proved that vacuum was not the reason, and the fact, the 'saddle shape' curve may be regarded as a HRS/IRR (hyper-radiosensitivity/increased radiaoresistance) effect caused by low dose irradiation. But Streptomyces albus UV irradiated after vacuum treatment only showed IRR effect or hormesis (survival rate >100%). The streptomycin resistance mutation of Streptomyces albus caused by low energy N + ion implantation and UV irradiation was also studied. the results showed that UV radiation is one effective means for streptomyces albus breeding. (authors)

  11. Impact of shortwave ultraviolet (UV-C) radiation on the antioxidant activity of thyme (Thymus vulgaris L.).

    Science.gov (United States)

    Dogu-Baykut, Esra; Gunes, Gurbuz; Decker, Eric Andrew

    2014-08-15

    Thyme is a good source of antioxidant compounds but it can be contaminated by microorganisms. An experimental fluid bed ultraviolet (UV) reactor was designed for microbial decontamination of thyme samples and the effect of shortwave ultraviolet light (UV-C) radiation on antioxidant properties of thyme was studied. Samples were exposed to UV-C radiation for 16 or 64 min. UV-C treatment led to 1.04 and 1.38 log CFU/g reduction of total aerobic mesophilic bacteria (TAMB) counts. Hunter a(∗) value was the most sensitive colour parameter during UV-C treatment. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of extracts was not significantly affected by UV-C. Addition of thyme extracts at 0.15 and 0.3 μmol GAE/ml emulsion delayed the formation of lipid hydroperoxides and headspace hexanal in the 5.0%(wt) corn oil-in-water emulsion from 4 to 9 and 14 days, respectively. No significant changes in oxidation rates were observed between UV-C treated and untreated samples at same concentrations. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. The protective roles of TiO2 nanoparticles against UV-B toxicity in Daphnia magna.

    Science.gov (United States)

    Liu, Jie; Wang, Wen-Xiong

    2017-09-01

    Aquatic environments are increasingly under environmental stress due to ultraviolet (UV) radiation and potential inputs of nanoparticles with intense application of nanotechnology. In this study, we investigated the interaction between UV-B radiation and titanium nanoparticles (TiO 2 -NPs) in a model freshwater cladoceran Daphnia magna. UV-B toxicity to Daphnia magna was examined when the daphnids were exposed to a range of TiO 2 -NPs concentrations with an initial 5 or 10min of 200μW/cm 2 UV-B radiation. In addition, UV-B toxicity was also examined in the presence of TiO 2 -NPs in the body of daphnids. Our results demonstrated that the daphnid mortality under UV-B radiation decreased significantly in the presence of TiO 2 -NPs both in the water and in the body, indicating that TiO 2 -NPs had some protective effects on D. magna against UV-B. Such protective effect was mainly caused by the blockage of UV-B by TiO 2 -NPs adsorption. UV-B produced reactive oxygen species (ROS) in the water and in the daphnids, which was not sufficient to cause mortality of daphnids over short periods of radiation. Previous studies focused on the effects of TiO 2 -NPs on the toxicity of total UV radiation, and did not attempt to differentiate the potential diverse roles of UV-A and UV-B. Our study indicated that TiO 2 -NPs may conversely protect the UV-B toxicity to daphnids. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. Determine the Intensity of UV Radiation and H2O2 on the Removal of Methylene Blue from Synthetic Wastewater

    Directory of Open Access Journals (Sweden)

    Mehdi Hosseini

    2015-03-01

    Full Text Available Background: There is a tremendous amount of color in textile wastewater that discharge it to the environment can cause a lot of environmental problems. The aim of this study is to evaluate the photocatalytic process UV/H2O2 to remove methylene blue dye from synthetic wastewater. Methods: UVC lamp was used as light source. In this study the effect of UV light intensity, irradiation distance, hydrogen peroxide concentration, and reaction time on the removal of methylene blue from aqueous solutions were studied. Data was analyzed by SPSS 18 and excel software. Results: The result showed that with increasing concentration of hydrogen peroxide, the color removal increases. Color removal changes were negligible at H2O2 concentration more than 5 mM. After 10 minutes reaction time at H2O2 concentration of 1 mm, efficiency of UVC/H2O2 is equal to 36.6%. Whilst at 5 mM concentration, removal efficiency is 89.2%. By increasing intensity of UV radiation, dye removal measure is also was increased, as the highest percentage of dye removal was obtained at 24 W radiation intensity. Conclusion: in present study, type of radiation and measure of hydrogen peroxide were the main factors in removal of methylene blue. Due to high efficiency of UVC/H2O2 process in removal of dye from aqueous solution, this method can use as an efficient process for removal of dye.

  14. Assessment of integrated solar ultraviolet radiation by PM-355 detectors

    International Nuclear Information System (INIS)

    Abu-Jarad, F.; Al-Jarallah, M.I.; Elhadidy, M.A.; Shaahid, S.M.; Fazal-ur-Rehman

    2000-01-01

    The increase in environmental solar UV radiation due to depletion of ozone layer is a recent challenge to human health (skin cancer and eye effects) in countries having clear skies. Therefore, applying integrated, passive and inexpensive techniques to assess solar UV radiation is very much essential. Measurements of environmental solar UV radiation in Dhahran, Saudi Arabia area were carried out for a period of two months in the summer period in 1996 using two techniques in parallel namely: passive nuclear track detectors and active solar UV radiometers. Some of the nuclear track detectors were mounted in different conditions such as: under shadow band, on solar tracking mechanism following the solar rays. Others were mounted on perpendicular, tilted and horizontal surfaces in sunlight. All detectors were attached to a wooden background of the same thickness (0.5 cm) to eliminate interference of the heat effect of various support materials and have uniformity of the support materials. The assessment was carried out for different periods extending from two to nine weeks continuously. The investigated period covered the hottest months in Saudi Arabia (July and August) when the sky was clear of clouds. The results indicate linear correlation between alpha track diameters and the integrated exposure to solar UV as measured by the solar UV radiometer for all nuclear track detector positions and orientations. The highest slope has been observed for the detectors placed on solar tracking mechanism following the solar rays and the lowest from detectors oriented under the shadow band on horizontal position (measuring the diffused UV radiation only). The results show that most of the measured UV radiation (60%) were from the diffused UV radiation. The characteristics of the upper layer of the detectors are changed after chemical etching very quickly, with increase in the exposure time to UV solar radiation at certain orientation. The results encourage the use of nuclear track

  15. Assessment of integrated solar ultraviolet radiation by PM-355 detectors

    Energy Technology Data Exchange (ETDEWEB)

    Abu-Jarad, F.; Al-Jarallah, M.I.; Elhadidy, M.A.; Shaahid, S.M.; Fazal-ur-Rehman

    2000-06-01

    The increase in environmental solar UV radiation due to depletion of ozone layer is a recent challenge to human health (skin cancer and eye effects) in countries having clear skies. Therefore, applying integrated, passive and inexpensive techniques to assess solar UV radiation is very much essential. Measurements of environmental solar UV radiation in Dhahran, Saudi Arabia area were carried out for a period of two months in the summer period in 1996 using two techniques in parallel namely: passive nuclear track detectors and active solar UV radiometers. Some of the nuclear track detectors were mounted in different conditions such as: under shadow band, on solar tracking mechanism following the solar rays. Others were mounted on perpendicular, tilted and horizontal surfaces in sunlight. All detectors were attached to a wooden background of the same thickness (0.5 cm) to eliminate interference of the heat effect of various support materials and have uniformity of the support materials. The assessment was carried out for different periods extending from two to nine weeks continuously. The investigated period covered the hottest months in Saudi Arabia (July and August) when the sky was clear of clouds. The results indicate linear correlation between alpha track diameters and the integrated exposure to solar UV as measured by the solar UV radiometer for all nuclear track detector positions and orientations. The highest slope has been observed for the detectors placed on solar tracking mechanism following the solar rays and the lowest from detectors oriented under the shadow band on horizontal position (measuring the diffused UV radiation only). The results show that most of the measured UV radiation (60%) were from the diffused UV radiation. The characteristics of the upper layer of the detectors are changed after chemical etching very quickly, with increase in the exposure time to UV solar radiation at certain orientation. The results encourage the use of nuclear track

  16. The Ultraviolet radiation (UV-C for the microbiological stabilization of red wine

    Directory of Open Access Journals (Sweden)

    Matias Fábio

    2016-01-01

    Full Text Available The traditional procedure for the control of the microbiological stability of wine consists of the addition of sulfur dioxide (SO2, which acts as an antimicrobial agent and also as an antioxidant. The search for alternative methods of microbiological control is important and necessary, since SO2 is a potential allergen and consumers are increasingly looking for healthier and preservative free products. Ultraviolet radiation was tested as an innovative technology that can help reduce the amount of sulphur dioxide used in winemaking. The object of this study was to optimize the process conditions compared to the results obtained previously, and to evaluate the efficiency of microbiological stabilization and its influence on the physico-chemical characteristics, the phenolic composition and sensory profile. Thus, red wine with very low content of sulphur dioxide was subjected to UV-C radiation in two different doses 424J/l e 778J/l, and the preparation of a control wine was carried out to which 30 mg/l sulfur dioxide was added. The wines (control=UV0, UV1 and UV2 were analyzed over time (from 0 to 4 months. The results show that treatment with a lower dosage is effective in the microbiological control of the product. The wines subjected to treatment with UV-C showed an increase in intensity of colour, and the treatment does not affect the flavour and taste of the wine.

  17. In the shadow of the Cosmetic Directive--inconsistencies in EU environmental hazard classification requirements for UV-filters.

    Science.gov (United States)

    Sobek, A; Bejgarn, S; Rudén, C; Molander, L; Breitholtz, M

    2013-09-01

    UV-filters are chemicals with potentially environmental hazardous properties. In the European Union (EU), UV-filters contained in sunscreen products are currently regulated by the Cosmetic Directive (from July 2013 by the Cosmetic Products Regulation). Environmental hazard classifications according to the regulation on classification, labelling and packaging of substances and mixtures (CLP) must be determined for UV-filters contained in industrial chemical products, whereas UV-filters contained in sunscreens are exempted from CLP. In this study we determined the potential environmental hazard classifications of UV-filters and sunscreen products if the CLP regulation was to be required for cosmetic products. Two sunscreen products were evaluated in accordance with the aquatic environmental hazard criteria for mixtures. The results highlight that the inconsistencies in the current EU regulation of UV filters hamper the risk management of environmental hazards of UV filters used in cosmetic products. Almost 50% of the investigated UV-filters approved for use in cosmetic products on the European market according to the current Cosmetic Directive were identified to meet the CLP classification as being hazardous to the aquatic environment. Assuming a worst-case scenario, the two examined sunscreens could both be classified as hazardous to the aquatic environment with long-lasting effects according to CLP classification criteria. Hence, if the CLP regulation was applicable to sunscreen products, both brands could potentially be labelled with the environmental hazard pictogram and associated hazard and precautionary statements. Including cosmetic products, and thereby sunscreens, in the CLP regulation would contribute to a more harmonized and transparent regulation of potentially hazardous substances on the EU market. Copyright © 2013 Elsevier B.V. All rights reserved.

  18. Fabrication and characterization of UV-emitting nanoparticles as novel radiation sensitizers targeting hypoxic tumor cells

    Science.gov (United States)

    Squillante, Michael R.; Jüstel, Thomas; Anderson, R. Rox; Brecher, Charles; Chartier, Daniel; Christian, James F.; Cicchetti, Nicholas; Espinoza, Sara; McAdams, Daniel R.; Müller, Matthias; Tornifoglio, Brooke; Wang, Yimin; Purschke, Martin

    2018-06-01

    Radiation therapy is one of the primary therapeutic techniques for treating cancer, administered to nearly two-thirds of all cancer patients. Although largely effective in killing cancer cells, radiation therapy, like other forms of cancer treatment, has difficulty dealing with hypoxic regions within solid tumors. The incomplete killing of cancer cells can lead to recurrence and relapse. The research presented here is investigating the enhancement of the efficacy of radiation therapy by using scintillating nanoparticles that emit UV photons. UV photons, with wavelengths between 230 nm and 280 nm, are able to inactivate cells due to their direct interaction with DNA, causing a variety of forms of damage. UV-emitting nanoparticles will enhance the treatment in two ways: first by generating UV photons in the immediate vicinity of cancer cells, leading to direct and oxygen-independent DNA damage, and second by down-converting the applied higher energy X-rays into softer X-rays and particles that are more efficiently absorbed in the targeted tumor region. The end result will be nanoparticles with a higher efficacy in the treatment of hypoxic cells in the tumor, filling an important, unmet clinical need. Our preliminary experiments show an increase in cell death using scintillating LuPO4:Pr nanoparticles over that achieved by the primary radiation alone. This work describes the fabrication of the nanoparticles, their physical characterization, and the spectroscopic characterization of the UV emission. The work also presents in vitro results that demonstrate an enhanced efficacy of cell killing with x-rays and a low unspecific toxicity of the nanoparticles.

  19. Effect of nutrient supply on photosynthesis and pigmentation to short-term stress (UV radiation) in Gracilaria conferta (Rhodophyta)

    International Nuclear Information System (INIS)

    Figueroa, F.L.; Israel, A.; Neori, A.; Martinez, B.; Malta, E.J.; Put, A.; Inken, S.; Marquardt, R.; Abdala, R.; Korbee, N.

    2010-01-01

    The effects of increased photosynthetic active radiation (PAR), UV radiation (UVR), and nutrient supply on photosynthetic activity, pigment content, C:N ratio and biomass yield were studied in tank cultivated Gracilaria conferta (Rhodophyta). Electron transport rate (ETR) and biliprotein content were higher under high nutrient supply (HNS), obtained from fishpond effluents, compared to low nutrient supply (LNS), in contrast to mycosporine-like amino acids (MAAs) dynamic. The high MAA content in LNS-algae could be explained by higher UVR penetration in the thallus and by the competition for the use of nutrients with other processes. Effective quantum yield decreased after short-term exposure to high irradiance whereas full recovery in shade was produced only under slightly heat shock. UVA radiation provoked an additional decrease in photosynthesis under high water temperature. UVB radiation reversed UVA's negative effect mainly with HNS. Results support that nutrient-sufficiency help G. conferta to resist environmental changes as short-term temperature increase.

  20. A Survey of the Potential Effects of Increasing UV-B Radiation on the Biosphere. Revision

    National Research Council Canada - National Science Library

    Martin, L

    1998-01-01

    ..., and an increase in UV-B radiation at the surface of the Earth. An increase in UV-B on average would increase the incidence rate of non-melanoma skin cancer worldwide, with an unproved but likely increase in melanoma skin cancer...

  1. Luminescent emission of LiF: Mg, Ti exposed to UV radiation

    International Nuclear Information System (INIS)

    Estrada G, A.; Castano M, V.M.; Cruz Z, E.; Garcia F, F.

    2002-01-01

    It was investigated the luminescent emission stimulated by heat (Tl) of LiF: Mg, Ti crystals which were exposed to UV radiation coming from a mercury lamp. Since this crystal depends on the thermal history, it has been used a thermal treatment consisting of a baking at 380 C during one hour for each reading and they were irradiated with UV. The brilliance curves between 5 and 840 minutes of exposure in the face of UV light were obtained. An important loss in the response, starting from 150 minutes of irradiation was observed. Also the relative intensity of the brilliance curve decay when the crystals being stored in darkness and room temperature conditions, which is according to the results in the literature about. (Author)

  2. Structural and functional changes in catalase induced by near-UV radiation

    International Nuclear Information System (INIS)

    Zigman, S.; Schultz, J.B.; McDaniel, T.

    1996-01-01

    Part one of this study shows that exposure of purified beef liver catalase in buffered solutions to BL lamps that provide a mixture of 99% UVA and 1% UVB (to be labeled UV A ) alters its chemistry and enzymatic activity. Thus, its spectral absorbance lose detail, it aggregated and exhibited a lower isoelectric point and its enzymatic activity was substantially reduced. These photochemically induced changes were increased by irradiation in phosphate buffer or in physiological medium (minimal essential medium) containing riboflavin and tryptophan. Neither α-tocopherol nor deferoxamine were protective against these UV A -induced changes in pure catalase. We further investigated the effect of UV A radiation on the activity of catalase in cultured lens epithelial cells and the protective effects of antioxidants. (Author)

  3. The response of antioxidant systems in Nostoc sphaeroides against UV-B radiation and the protective effects of exogenous antioxidants

    Science.gov (United States)

    Wang, Gaohong; Hu, Chunxiang; Li, Dunhai; Zhang, Delu; Li, Xiaoyan; Chen, Kun; Liu, Yongding

    UV radiation is one of many harmful factors found in space that are detrimental to organisms on earth in space exploration. In the present work, we examined the role of antioxidant system in Nostoc sphaeroides Kütz (Cyanobacterium) and the effects of exogenously applied antioxidant molecules on its photosynthetic rate under UV-B radiation. It was found that UV-B radiation promoted the activity of antioxidant system to protect photosystem II (PSII) and exogenously applied antioxidant: sodium nitroprusside (SNP) and N-acetylcysteine (NAC) had an obvious protection on PSII activity under UV-B radiation. The activity of superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), peroxidase (POD, EC 1.11.1.7) and content of MDA (malondialdehyde) and ASC (ascorbate) were improved by 0.5 mM and 1 mM SNP, but 0.1 mM SNP decreased the activity of antioxidant system. Addition of exogenous NAC decreased the activity of SOD, POD, CAT and the content MDA and ASC. In contrast, exogenously applied NAC increased GSH content. The results suggest that exogenous SNP and NAC may protect algae by different mechanisms: SNP may play double roles as both sources of reactive free radicals as well as ROS scavengers in mediating the protective role of PSII on algae under UV-B radiation. On the other hand, NAC functions as an antioxidant or precursor of glutathione, which could protect PSII directly from UV-B radiation.

  4. He-Ne laser treatment improves the photosynthetic efficiency of wheat exposed to enhanced UV-B radiation

    International Nuclear Information System (INIS)

    Chen, Huize; Han, Rong

    2014-01-01

    The level of ultraviolet-B (UV-B) radiation on the Earth’s surface has increased due to depletion of the ozone layer. Here, we explored the effects of continuous wave He-Ne laser irradiation (632 nm, 5 mW mm –2 , 2 min d –1 ) on the physiological indexes of wheat seedlings exposed to enhanced UV-B radiation (10 KJ m –2 d –1 ) at the early growth stages. Wheat seedlings were irradiated with enhanced UV-B, He-Ne laser treatment or a combination of the two. Enhanced UV-B radiation had deleterious effects on wheat photosynthesis parameters including photosystem II (chlorophyll content, Hill reaction, chlorophyll fluorescence parameters, electron transport rate (ETR), and yield), the thylakoid (optical absorption ability, cyclic photophosphorylation, Mg 2+ -ATPase, and Ca 2+ -ATPase) and some enzymes in the dark reaction (phosphoenolpyruvate carboxylase (PEPC), carbonic anhydrase (CA), malic dehydrogenase (MDH), and chlorophyllase). These parameters were improved in UV-B-exposed wheat treated with He-Ne laser irradiation; the parameters were near control levels and the enzyme activities increased, suggesting that He-Ne laser treatment partially alleviates the injury caused by enhanced UV-B irradiation. Furthermore, the use of He-Ne laser alone had a favourable effect on seedling photosynthesis compared with the control. Therefore, He-Ne laser irradiation can enhance the adaptation capacity of crops. (paper)

  5. A ΔdinB mutation that sensitizes Escherichia coli to the lethal effects of UV- and X-radiation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Mei-Chong W.; Franco, Magdalena; Vargas, Doris M. [Department of Biological Sciences, San Jose State University, San Jose, CA 95192 (United States); Hudman, Deborah A. [Department of Microbiology and Immunology, A.T. Still University, Kirksville College of Osteopathic Medicine, Kirksville, MO 63501 (United States); White, Steven J. [Department of Biological Sciences, San Jose State University, San Jose, CA 95192 (United States); Fowler, Robert G., E-mail: rfowler@sjsu.edu [Department of Biological Sciences, San Jose State University, San Jose, CA 95192 (United States); Sargentini, Neil J. [Department of Microbiology and Immunology, A.T. Still University, Kirksville College of Osteopathic Medicine, Kirksville, MO 63501 (United States)

    2014-05-15

    Highlights: • We describe Δ(dinB-yafN)883(::kan), a novel dinB allele, referred to as ΔdinB883, a deletion that sensitizes E. coli cells to UV irradiation. • This UV radiation sensitivity is most acute in the early logarithmic phase of culture growth. • This UV radiation sensitivity is completely dependent upon a functional umuDC operon. • Sequencing reveals ΔdinB883 retains the proximal 161 nucleotides, i.e., 54 amino acids, of the wild-type sequence. • The ΔdinB883 mutant is hypothesized to produce a peptide of 83 amino acids, DinB883, that compromises UmuDC function. - Abstract: The DinB (PolIV) protein of Escherichia coli participates in several cellular functions. We investigated a dinB mutation, Δ(dinB-yafN)883(::kan) [referred to as ΔdinB883], which strongly sensitized E. coli cells to both UV- and X-radiation killing. Earlier reports indicated dinB mutations had no obvious effect on UV radiation sensitivity which we confirmed by showing that normal UV radiation sensitivity is conferred by the ΔdinB749 allele. Compared to a wild-type strain, the ΔdinB883 mutant was most sensitive (160-fold) in early to mid-logarithmic growth phase and much less sensitive (twofold) in late log or stationary phases, thus showing a growth phase-dependence for UV radiation sensitivity. This sensitizing effect of ΔdinB883 is assumed to be completely dependent upon the presence of UmuDC protein; since the ΔdinB883 mutation did not sensitize the ΔumuDC strain to UV radiation killing throughout log phase and early stationary phase growth. The DNA damage checkpoint activity of UmuDC was clearly affected by ΔdinB883 as shown by testing a umuC104 ΔdinB883 double-mutant. The sensitivities of the ΔumuDC strain and the ΔdinB883 ΔumuDC double-mutant strain were significantly greater than for the ΔdinB883 strain, suggesting that the ΔdinB883 allele only partially suppresses UmuDC activity. The ΔdinB883 mutation partially sensitized (fivefold) uvrA and uvr

  6. The dominant allele Aft induces a shift from flavonol to anthocyanin production in response to UV-B radiation in tomato fruit.

    Science.gov (United States)

    Catola, Stefano; Castagna, Antonella; Santin, Marco; Calvenzani, Valentina; Petroni, Katia; Mazzucato, Andrea; Ranieri, Annamaria

    2017-08-01

    The introgression of the A ft allele into domesticated tomato induced a shift from flavonol to anthocyanin production in response to UV-B radiation, while the hp - 1 allele negatively influenced the response of flavonoid biosynthesis to UV-B. Introgression of the dominant allele Anthocyanin fruit (Aft) from Solanum chilense induces anthocyanin accumulation in the peel of tomato (Solanum lycopersicum L.) fruit. UV-B radiation can influence plant secondary metabolism regulating the expression of several genes, among which those involved in flavonoid biosynthesis. Here, we investigated whether post-harvest UV-B treatment could up-regulate flavonoid production in tomato fruits and whether the Aft allele could affect flavonoid biosynthesis under UV-B radiation. Mature green fruits of an anthocyanin-rich tomato mutant line (SA206) and of its wild-type reference, cv. Roma, were daily subjected to post-harvest UV-B treatment until full ripening. Up-regulation of CHS and CHI transcription by UV-B treatment induced flavonoid accumulation in the peel of cv. Roma. Conversely, UV-B decreased the total flavonoid content and CHS transcript levels in the SA206 peel. SA206 being a double mutant containing also hp-1 allele, we investigated also the behavior of hp-1 fruit. The decreased peel flavonoid accumulation and gene transcription in response to UV-B suggest that hp-1 allele is involved in the marked down-regulation of the flavonoid biosynthesis observed in SA206 fruit. Interestingly, in SA206, UV-B radiation promoted the synthesis of delphinidin, petunidin, and malvidin by increasing F3'5'H and DFR transcription, but it decreased rutin production, suggesting a switch from flavonols to anthocyanins. Finally, although UV-B radiation does not reach the inner fruit tissues, it down-regulated flavonoid biosynthesis in the flesh of both genotypes. This study provides, for the first time, evidence that the presence of the functional Aft allele, under UV-B radiation, redirects

  7. Pigmented binding material which can be photo-polymerised by UV radiation

    Energy Technology Data Exchange (ETDEWEB)

    McGinniss, V C

    1977-10-20

    The invention concerns the improvement of a binding material which can be photo-polymerised by UV radiation, which contains aromatic hydrocarbons and ethylene unsaturated film material. Coatings with a film thickness of about 2 x 5 to 21 x 5 ..mu..m are made from this. In order to harden ethylene unsaturated binding material in the presence of pigments which are made opaque by UV and laser energy and to polymerise it, an additive consisting of a synergistic sensitising mixture is necessary, which consists of three components, all of whose variations are described in detail.

  8. The sensitivity of sunflower (Helianthus annuus L.) plants to UV-B radiation is altered by nitrogen status

    OpenAIRE

    Cechin, Inês; Gonzalez, Gisely Cristina; Corniani, Natália; Fumis, Terezinha de Fátima

    2018-01-01

    ABSTRACT: Interaction effects between nitrogen and UV-B radiation were studied in sunflower (Helianthus annuus L. variety IAC-Iarama) plants grown in a greenhouse under natural photoperiod conditions. Plants were irradiated with 0.8W m-2 (control) or 8.0W m-2 (+UV-B) of UV-B radiation for 7h per day. The plants were grown in pots containing vermiculite and watered with 70% of full strength nitrogen-free Long Ashton solution, containing either low (42.3ppm) or high (282ppm) nitrogen as ammoniu...

  9. Accelerated degradation by UV radiation of adhesive materials used in solar equipment

    International Nuclear Information System (INIS)

    Tilca, F.; Acosta, D; Barcena, H.; Suarez, H.; Cadena, C.; Bolzi, Claudio

    2003-01-01

    Several materials which are used as common adhesives in photovoltaic cells, were tested in order to study their stability. Accelerated degradation effects were produced using high radiation doses of UV-C and UV-b in a previously described camera at different times. The exposed and unexposed films were studied by transmittance, X-ray diffraction and infrared. The results are in agreement with complex degradation process at long exposition times, while transmittance doesn't change significantly. (author)

  10. The effects of UV radiation during the vegetative period on antioxidant compounds and postharvest quality of broccoli (Brassica oleracea L.).

    Science.gov (United States)

    Topcu, Yasin; Dogan, Adem; Kasimoglu, Zehra; Sahin-Nadeem, Hilal; Polat, Ersin; Erkan, Mustafa

    2015-08-01

    In this study, the effects of supplementary UV radiation during the vegetative period on antioxidant compounds, antioxidant activity and postharvest quality of broccoli heads during long term storage was studied. The broccolis were grown under three different doses of supplementary UV radiation (2.2, 8.8 and 16.4 kJ/m(2)/day) in a soilless system in a glasshouse. Harvested broccoli heads were stored at 0 °C in modified atmosphere packaging for 60 days. The supplementary UV radiation (280-315 nm) during the vegetative period significantly decreased total carotenoid, the chlorophyll a and chlorophyll b content but increased the ascorbic acid, total phenolic and flavonoid contents of broccolis. All supplementary UV treatments slightly reduced the antioxidant activity of the broccolis, however, no remarkable change was observed between 2.2 and 8.8 kJ/m(2) radiation levels. The sinigrin and glucotropaeolin contents of the broccolis were substantially increased by UV treatments. The prolonged storage period resulted in decreased ascorbic acid, total phenolic and flavonoid contents, as well as antioxidant activity. Discoloration of the heads, due to decreased chlorophyll and carotenoid contents, was also observed with prolonged storage duration. Glucosinolates levels showed an increasing tendency till the 45th day of storage, and then their levels started to decline. The weight loss of broccoli heads during storage progressively increased with storage time in all treatments. Total soluble solids, solids content and titratable acidity decreased continuously during storage. Titratable acidity was not affected by UV radiation doses during the storage time whereas soluble solids and solids content (dry matter) were significantly affected by UV doses. Supplementary UV radiation increased the lightness (L*) and chroma (C*) values of the broccoli heads. Pre-harvest UV radiation during vegetative period seems to be a promising tool for increasing the beneficial health components

  11. Radiation and environmental radioactivity

    International Nuclear Information System (INIS)

    Muhamat Omar; Ismail Sulaiman; Zalina Laili

    2015-01-01

    This book is written based on 25 years authors experience especially in scientifc research of radiation and environmental radioactivity field at Malaysian Nuclear Agency (Nuklear Malaysia). Interestingly, from the authors experience in managing the services and consultancies for radiological environmental monitoring, it is also helpful in preparing the ideas for this book. Although this book focuses on Malaysian radiation information environmental radioactivity, but the data collected by the international bodies are also included in this book.

  12. Biochemical and ultrastructural changes in pollen of Zea mays L. grown under enhanced UV-B radiation

    International Nuclear Information System (INIS)

    Santos, A.; Almeida, J.M.; Santos, I.; Salema, R.

    1998-01-01

    The influence of ultraviolet-B (UV-B) radiation on the development of the male gametophyte was studied in Zea mays L. cv. LG12 grown in a growth chamber under PAR light supplemented with UV-B radiation and compared with a second set of plants grown under PAR light. Pollen samples collected from both groups of plants were cultured on germination medium and it was found that UV-B had no effect on pollen germination. Total pollen protein content was not affected but UV-B absorbing pigments increased. Some ultrastructural alterations were observed in pollen and pollen tubes, in particular large amounts of electron dense deposits were seen throughout the cytoplasm and in association with the pollen wall. In mature spikes of UV-B treated plants, anthers retained numerous pollen grains in their loculi while anthers of control plants were almost empty. UV-B treatment delayed flowering by 2±3 d. These results show that UV-B treatment of maize plants interferes with flowering, pollen ultrastructure and anther maturation even though pollen germination is unaffected. The significant increase of UV-B absorbing pigments in pollen grains could represent a defence mechanism that enables plants to complete their reproductive cycle. (author)

  13. Ambient UV-B radiation reduces PSII performance and net photosynthesis in high Arctic Salix arctica

    DEFF Research Database (Denmark)

    Albert, Kristian Rost; Mikkelsen, Teis Nørgaard; Ro-Poulsen, Helge

    2011-01-01

    Ambient ultraviolet-B (UV-B) radiation potentially impacts the photosynthetic performance of high Arctic plants. We conducted an UV-B exclusion experiment in a dwarf shrub heath in NE Greenland (74°N), with open control, filter control, UV-B filtering and UV-AB filtering, all in combination......, nitrogen and UV-B absorbing compounds. Compared to a 60% reduced UV-B irradiance, the ambient solar UV-B reduced net photosynthesis in Salix arctica leaves fixed in the 45° position which exposed leaves to maximum natural irradiance. Also a reduced Calvin Cycle capacity was found, i.e. the maximum rate...... across position in the vegetation. These findings add to the evidence that the ambient solar UV-B currently is a significant stress factor for plants in high Arctic Greenland....

  14. Chromatin damage induced by fast neutrons or UV laser radiation

    Energy Technology Data Exchange (ETDEWEB)

    Radu, L.; Constantinescu, B.; Gazdaru, D.; Mihailescu, I

    2002-07-01

    Chromatin samples from livers of Wistar rats were subjected to fast neutron irradiation in doses of 10-100 Gy or to a 248 nm excimer laser radiation, in doses of 0.5-3 MJ.m{sup -2}. The action of the radiation on chromatin was monitored by chromatin intrinsic fluorescence and fluorescence lifetimes (of bound ethidium bromide to chromatin) and by analysing fluorescence resonance energy transfer between dansyl chloride and acridine orange coupled to chromatin. For the mentioned doses of UV excimer laser radiation, the action on chromatin was more intense than in the case of fast neutrons. The same types of damage are produced by the two radiations: acidic and basic destruction of chromatin protein structure, DNA strand breaking and the increase of the distance between DNA and proteins in chromatin. (author)

  15. Chromatin damage induced by fast neutrons or UV laser radiation

    International Nuclear Information System (INIS)

    Radu, L.; Constantinescu, B.; Gazdaru, D.; Mihailescu, I.

    2002-01-01

    Chromatin samples from livers of Wistar rats were subjected to fast neutron irradiation in doses of 10-100 Gy or to a 248 nm excimer laser radiation, in doses of 0.5-3 MJ.m -2 . The action of the radiation on chromatin was monitored by chromatin intrinsic fluorescence and fluorescence lifetimes (of bound ethidium bromide to chromatin) and by analysing fluorescence resonance energy transfer between dansyl chloride and acridine orange coupled to chromatin. For the mentioned doses of UV excimer laser radiation, the action on chromatin was more intense than in the case of fast neutrons. The same types of damage are produced by the two radiations: acidic and basic destruction of chromatin protein structure, DNA strand breaking and the increase of the distance between DNA and proteins in chromatin. (author)

  16. Interfacial Shear Strength Evaluation of Pinewood Residue/High-Density Polyethylene Composites Exposed to UV Radiation and Moisture Absorption-Desorption Cycles

    Directory of Open Access Journals (Sweden)

    Soledad C. Pech-Cohuo

    2016-03-01

    Full Text Available In outdoor applications, the mechanical performance of wood-plastic composites (WPCs is affected by UV radiation, facilitating moisture intake and damaging the wood-polymer interfacial region. The purpose of this study was to evaluate the effect of moisture absorption-desorption cycles (MADCs, and the exposure to UV radiation on the interfacial shear strength (IFSS of WPCs with 40% pinewood residue and 60% high-density polyethylene. One of the WPCs incorporated 5% coupling agent (CA with respect to wood content. The IFSS was evaluated following the Iosipescu test method. The specimens were exposed to UV radiation using an accelerated weathering test device and subsequently subjected to four MADCs. Characterization was also performed by scanning electron microscopy (SEM and Fourier transform infrared spectroscopy (FTIR. The absorption and desorption of moisture was slower in non-UV-irradiated WPCs, particularly in those with the CA. The UV radiation did not significantly contribute to the loss of the IFSS. Statistically, the CA had a favorable effect on the IFSS. Exposure of the samples to MADCs contributed to reduce the IFSS. The FTIR showed lignin degradation and the occurrence of hydrolysis reactions after exposure to MADCs. SEM confirmed that UV radiation did not significantly affect the IFSS.

  17. Heated water and UV-C radiation to post harvest control of Cryptosporiopsis perennans on apples; Agua aquecida e radiacao UV-C no controle pos-colheita de Cryptosporiopsis perennans em macas

    Energy Technology Data Exchange (ETDEWEB)

    Bartnicki, Vinicius Adao; Amarante, Cassandro Vidal Talamini do, E-mail: vinibart@hotmail.co, E-mail: amarante@cav.udesc.b [Universidade do Estado de Santa Catarina (UDESC), Lages, SC (Brazil). Centro de Ciencias Agroveterinarias. Dept. de Agronomia; Valdebenito-Sanhueza, Rosa Maria, E-mail: rosamaria@m2net.com.b [Proterra Engenharia Agronomica, Vacaria, RS (Brazil); Castro, Luis Antonio Suita de, E-mail: suita@cpact.embrapa.b [EMBRAPA Clima Temperado, Pelotas, RS (Brazil); Rizzatti, Mara Regina; Souza, Joao Antonio Vargas de, E-mail: marar@pucrs.b [Pontificia Universidade Catolica do Rio Grande do Sul (PUC-RS), Porto Alegre, RS (Brazil). Centro em Pesquisa e Desenvolvimento em Fisica. Grupo de Fisica das Radiacoes

    2010-02-15

    The objective of this work was to assess the colonization of Cryptosporiopsis perennans in the epidermis of apples and the efficiency of heated water and UV-C radiation application to control this pathogen. In apples inoculated with C. perennans, the colonization of lenticels and adjacent areas by the pathogen was observed by electronic scanning microscopy. The sensitivity of C. perennans conidia was evaluated in aqueous suspension, at temperatures of 28, 45, 50 and 55 deg C for 15 and 30 s, and at UV.C radiation doses of 0.018, 0.037, 0.075, 0.150, 0.375, 0.750, 1.500 and 3.000 kJ m.2. The effects of UV.C radiation doses at 0.375, 0.750 and 1.500 kJ m.2 and heated water at 50 deg C, sprayed during 15 and 30 s were evaluated for controlling C. perennans in apples inoculated with the pathogen. The fungus produced abundant mycelium and conidia in lenticels and adjacent areas on the epidermis of the apples. The heated water at 50 deg C during 15 s and a 0.750 kJ m.2 UV.C radiation dose reduced conidia survival in more than 99%. Heated water sprayed at 50 deg C during 15 s and a UV.C radiation dose of 0.375 kJ m.2 control C. perennans in apples. (author)

  18. Urban forest influences on exposure to UV radiation and potential consequences for human health

    Science.gov (United States)

    Gordon M. Heisler

    2010-01-01

    This chapter explores the literature on ultraviolet (UV) irradiance in urban ecosystems with respect to the likely effects on human health. The focus was the question of whether the health effects of UV radiation should be included in the planning of landscape elements such as trees and shading structures, especially for high use pedestrian areas and school play...

  19. Combined effects of O3 and UV radiation on secondary metabolites and endogenous hormones of soybean leaves.

    Directory of Open Access Journals (Sweden)

    Bing Mao

    Full Text Available Enhanced ultraviolet radiation (UV and elevated tropospheric ozone (O3 may individually cause reductions in the growth and productivity of important agricultural crops. However, research regarding their combined effects on important agricultural crops is still scarce, especially on changes in secondary metabolites and endogenous hormones, which are important protective substances and signal components that control plant responses to environment stresses. In this study, using an experimental setup of open top chambers, we monitored the responses of seed yield per plant, leaf secondary metabolites and leaf endogenous hormones under the stress of elevated O3 and enhanced UV radiation individually, as well as their combined stress. The results indicated that elevated O3 (110 ± 10 nmol mol-1 for 8 hours per day and enhanced UV radiation (1.73 kJ h-1 m-2 significantly decreased seed yield per plant. Concentrations of rutin, queretin and total flavonoids were significantly increased under the elevated O3 treatment or the enhanced UV radiation treatment or the combination treatment at flowering and podding stages, and concentrations of rutin, queretin and total flavonoids showed significant correlations with seed yield per plant. Concentrations of ABA and IAA decreased under the three treatments. There was a significant positive correlation between the ABA concentration and seed yield and a negative correlation between the IAA concentration and seed yield. We concluded that the combined stress of elevated O3 and UV radiation significantly decreased seed yield per plant. Yield reduction was associated with changes in the concentrations of flavonoids, ABA and IAA in soybean leaves. The effects of the combined O3 and UV stress were always greater than those of the individual stresses alone.

  20. Ultraviolet-B (UV-B) radiation under high-temperature conditions affects growth of rice (cv. Koshihikari) after a young panicle formation stage

    International Nuclear Information System (INIS)

    Inaba, K.

    2005-01-01

    A japonica rice (cv. Koshihikari) was used to test the effects on blooming and ripening of UV-B radiation treatment combined with high temperature treatments (day/night, 35 / 30 deg C). Strong UV-B radiation (60.4 kJ/m(2) . day) slightly increased sterility. High temperatures and weak UV-B radiation (18.1 kJ/m(2) . day) applied together from two weeks before heading and from the heading day increased sterility and those applied from two weeks after heading decreased sterility. High temperature combined with strong UV-B radiation applied from two weeks before heading increased sterility and decreased the size of unhulled grain and anther length. The same treatment given from the heading stage greatly increased sterility and decreased anther length and pollen production, and that given two weeks after heading decreased unhulled grain weight. It also decreased photosynthetic rate in Flag leaves. A high temperature applied together with strong UV-B radiation had a synergistic effect causing poor growth; it increased the harmful effects of a high temperature and strong UV-B given separately, on the sterility and pollen formation

  1. Implementation of innovative pulsed xenon ultraviolet (PX-UV environmental cleaning in an acute care hospital

    Directory of Open Access Journals (Sweden)

    Fornwalt L

    2014-01-01

    Full Text Available Lori Fornwalt,1 Brad Riddell1,2 1Departments of Infection Prevention and Environmental Services, Trinity Medical Centre, Birmingham, AL, 2Environmental Services, Medical University of South Carolina, Charleston, SC, USA Abstract: It is widely acknowledged that the hospital environment is an important reservoir for many of the pathogenic microbes associated with health care-associated infections (HAIs. Environmental cleaning plays an important role in the prevention and containment of HAIs, in patient safety, and the overall experience of health care facilities. New technologies, such as pulsed xenon ultraviolet (PX-UV light systems are an innovative development for enhanced cleaning and decontamination of hospital environments. A portable PX-UV disinfection device delivers pulsed UV light to destroy microbial pathogens and spores, and can be used in conjunction with manual environmental cleaning. In addition, this technology facilitates thorough disinfection of hospital rooms in 10–15 minutes. The current study was conducted to evaluate whether the introduction of the PX-UV device had a positive impact on patient satisfaction. Satisfaction was measured using the Hospital Consumer Assessment of Healthcare Providers and Systems (HCAHPS survey. In 2011, prior to the introduction of the PX-UV system, patient HCAHPS scores for cleanliness averaged 75.75%. In the first full quarter after enhanced cleaning of the facility was introduced, this improved to 83%. Overall scores for the hospital rose from 76% (first quarter, 2011 to 87.6% (fourth quarter, 2012. As a result of this improvement, the hospital received 1% of at-risk reimbursement from the inpatient prospective payment system as well as additional funding. Cleanliness of the hospital environment is one of the questions included in the HCAHPS survey and one measure of patient satisfaction. After the introduction of the PX-UV system, the score for cleanliness and the overall rating of the

  2. Health hazards of UV radiation

    International Nuclear Information System (INIS)

    Matthes, R.

    1994-01-01

    The author describes the effects and health risks of UV exposure. This includes UV effects on the DNS, the eyes, the immune system, and the skin. Finally, recommendations are given for protection against excessive UV exposure on the basis of the IRPA/INIRC guidelines. (orig.) [de

  3. UV exposure in cars.

    Science.gov (United States)

    Moehrle, Matthias; Soballa, Martin; Korn, Manfred

    2003-08-01

    There is increasing knowledge about the hazards of solar and ultraviolet (UV) radiation to humans. Although people spend a significant time in cars, data on UV exposure during traveling are lacking. The aim of this study was to obtain basic information on personal UV exposure in cars. UV transmission of car glass samples, windscreen, side and back windows and sunroof, was determined. UV exposure of passengers was evaluated in seven German middle-class cars, fitted with three different types of car windows. UV doses were measured with open or closed windows/sunroof of Mercedes-Benz E 220 T, E 320, and S 500, and in an open convertible car (Mercedes-Benz CLK). Bacillus subtilis spore film dosimeters (Viospor) were attached to the front, vertex, cheeks, upper arms, forearms and thighs of 'adult' and 'child' dummies. UV wavelengths longer than >335 nm were transmitted through car windows, and UV irradiation >380 nm was transmitted through compound glass windscreens. There was some variation in the spectral transmission of side windows according to the type of glass. On the arms, UV exposure was 3-4% of ambient radiation when the car windows were shut, and 25-31% of ambient radiation when the windows were open. In the open convertible car, the relative personal doses reached 62% of ambient radiation. The car glass types examined offer substantial protection against short-wave UV radiation. Professional drivers should keep car windows closed on sunny days to reduce occupational UV exposure. In individuals with polymorphic light eruption, produced by long-wave UVA, additional protection by plastic films, clothes or sunscreens appears necessary.

  4. Radiation protection in the field of environmental protection

    International Nuclear Information System (INIS)

    Zhao Yamin

    2003-01-01

    The relationship of radiation protection with environmental protection, the sources that may give rise to the environmental radiation contamination, and the system of radiation protection and the fundamental principles and requirements for radiation environmental management are introduced. Some special radiation protection problems faced with in the radiation environmental management are discussed. (author)

  5. Epidermal transmittance and phenolic composition in leaves of atrazine-tolerant and atrazine-sensitive cultivars of Brassica napus grown under enhanced UV-B radiation

    International Nuclear Information System (INIS)

    Olsson, L.C.; Veit, M.; Bornman, J.F.

    1999-01-01

    Experiments were conducted on the atrazine-tolerant mutant Stallion and the atrazine-sensitive cv. Paroll of Brassica napus L., which were grown under either visible light or with the addition of UV-B radiation (280–320 nm) for 15 days. The mutant has been shown to be sensitive to high levels of visible light as compared to the atrazine-sensitive cultivar and therefore we wished to determine plant response to UV-B radiation with respect to potential pigment changes, certain anatomical features, radiation penetration and partial photosynthesis. With regard to pigment changes, we were particularly interested in whether the compositional shift in flavonol pigments under enhanced UV-B radiation, previously suggested to favour increased antioxidant activity, is confined to the adaxial epidermis, which generally receives most UV-B radiation or whether the pigment shift is also inducible in the abaxial epidermis.As was to be expected, the penetration of UV-B radiation (310 nm) was lower in the UV-B-exposed plants, which was correlated with an increased amount of UV-screening pigments in the adaxial and abaxial epidermal layers. The main flavonoid glycosides showed the largest shift from kaempferol to quercetin as aglycone moiety in the adaxial epidermal layer. However, in the abaxial epidermal layer the hydroxycinnamic acid (HCA) derivatives and kaempferol glycosides were predominant. Penetration of 430 nm light was higher after UV-B exposure, and probably contributed to the fact that photosynthetic efficiency of photosystem II was unchanged or higher after UV-B exposure. UV-B radiation decreased leaf area in the atrazine-tolerant mutant only. Both cultivars showed an increased leaf thickness after UV-B exposure due to cell elongation mainly of the palisade tissue. This was especially evident in the mutant

  6. A flavonoid mutant of barley (Hordeum vulgare L.) exhibits increased sensitivity to UV-B radiation in the primary leaf

    International Nuclear Information System (INIS)

    Reuber, S.; Bornman, J.F.; Weissenböck, G.

    1996-01-01

    The aim of the present investigation was to define the role of soluble flavonoids as UV-B protectants in the primary leaf of barley (Hordeum vulgare L.). For this purpose we used a mutant line (Ant 287) from the Carlsberg collection of proanthocyanidin-free barley containing only 7% of total extractable flavonoids in the primary leaf as compared to the mother variety (Hiege 550/75). Seven-day-old leaves from plants grown under high visible light with or without supplementary UV-B radiation were used for the determination of UV-B sensitivity. UV-B-induced changes were assessed from parameters of chlorophyll fluorescence of photosystem II, including initial and maximum fluorescence, apparent quantum yield, and photochemical and non-photochemical quenching. A quartz fibre-optic microprobe was used to evaluate the amount of potentially harmful UV-B (310 nm radiation) penetrating into the leaf as a direct consequence of flavonoid deficiency. Our data indicate an essential role of flavonoids in UV-B protection of barley primary leaves. In leaves of the mutant line grown under supplementary UV-B, an increase in 310nm radiation in the mesophyll and a strong decrease in the quantum yield of photosynthesis were observed as compared to the corresponding mother variety. Primary leaves of liege responded to supplementary UV-B radiation with a 30% increase in the major flavonoid saponarin and a 500% increase in the minor compound lutonarin. This is assumed to be an efficient protective response since no changes in variable chlorophyll fluorescence were apparent. In addition, a further reduction in UV-B penetration into the mesophyll was recorded in these leaves

  7. Oxidative stress and enzymatic scavenging of superoxide radicals induced by solar UV-B radiation in Ulva canopies from southern Spain

    Directory of Open Access Journals (Sweden)

    Kai Bischof

    2003-09-01

    Full Text Available The generation of reactive oxygen species (ROS and scavenging of the superoxide radical by superoxide dismutase (SOD was studied in mat-like canopies of the green macroalga Ulva rotundata Bliding in a tidal brine pond system in southern Spain. Artificial canopies were covered with different cut-off filters, generating different radiation conditions. ROS and SOD were assessed after three days of exposure. ROS induced lipid peroxidation depended on the position of individual thalli within the canopy and on radiation conditions. Samples exposed to the full solar spectrum were most affected, whereas samples either exposed to photosynthetically active radiation (PAR alone or UV radiation without PAR exhibited fewer peroxidation products. The activity of SOD appeared to be controlled by the impinging UV-A and UV-B radiation and also increased in response to oxidative stress. The results provide evidence for additive effects of high PAR and UV-B under field conditions and support the previously proposed hypothesis that UV-B effects are mediated by an inhibition of the xanthophyll cycle, which increases ROS production and, consequently, causes oxidative damage to components of the photosynthetic machinery, such as proteins and pigments.

  8. Effects of reducing the ambient UV-B radiation in the high Arctic on Salix arctica and Vaccinium uliginosum

    DEFF Research Database (Denmark)

    Albert, K.R.; Ro-Poulsen, H.; Mikkelsen, Teis Nørgaard

    2005-01-01

    , transmitting λ > 400 nm) were used to reduce UV-B radiation and UV-B+A respectively. A UV transparent film (Teflon, transmitting λ > 280 nm) and no film were used as controls. Field measurements showed that the plants under Teflon, Mylar and Lexan received app. 91%, 39% and 17% of the ambient UV-B irradiance...

  9. Acclimation to UV-B radiation and visible light in Lactuca sativa involves up-regulation of photosynthetic performance and orchestration of metabolome-wide responses.

    Science.gov (United States)

    Wargent, J J; Nelson, B C W; McGhie, T K; Barnes, P W

    2015-05-01

    UV-B radiation is often viewed as a source of stress for higher plants. In particular, photosynthetic function has been described as a common target for UV-B impairment; yet as our understanding of UV-B photomorphogenesis increases, there are opportunities to expand the emerging paradigm of regulatory UV response. Lactuca sativa is an important dietary crop species and is often subjected to rapid sunlight exposure at field transfer. Acclimation to UV-B and visible light conditions in L. sativa was dissected using gas exchange and chlorophyll fluorescence measurements, in addition to non-destructive assessments of UV epidermal shielding (SUV ). After UV-B treatment, seedlings were subjected to wide-range metabolomic analysis using liquid chromatography hybrid quadrupole time-of-flight high-resolution mass spectrometry (LC-QTOF-HRMS). During the acclimation period, net photosynthetic rate increased in UV-treated plants, epidermal UV shielding increased in both subsets of plants transferred to the acclimatory conditions (UV+/UV- plants) and Fv /Fm declined slightly in UV+/UV- plants. Metabolomic analysis revealed that a key group of secondary compounds was up-regulated by higher light conditions, yet several of these compounds were elevated further by UV-B radiation. In conclusion, acclimation to UV-B radiation involves co-protection from the effects of visible light, and responses to UV-B radiation at a photosynthetic level may not be consistently viewed as damaging to plant development. © 2014 John Wiley & Sons Ltd.

  10. Effects of UV radiation on genetic recombination

    International Nuclear Information System (INIS)

    Vlahovic, K.; Zahradka, D.; Petranovic, M.; Petranovic, D.

    1996-01-01

    We have used the model consisting of Escherichia coli cells and l phage to study the effects of UV radiation on genetic recombination. We found two radiation induced processes that reduce or inhibit genetic recombination. One such process leads to the inability of prophage to excise itself from the irradiated bacterial chromosome by the site-specific recombination. The other process was shown to inhibit a type of general recombination by which the prophage transfers one of its genetic markers to the infecting homologous phage. Loss of the prophage ability to take part in both site-specific and general recombination was shown to develop in recB + but not in recB cells. From this we infer that the loss of prophage recombinogenicity in irradiated cells is a consequence of one process in which RecBCD enzyme (the product of recB, recC and recD genes) plays an essential role. (author)

  11. Development of environmental radiation control technology

    International Nuclear Information System (INIS)

    Kim, Ingyu; Kim, Enhan; Keum, Dongkwon

    2012-04-01

    To develop the comprehensive environmental radiation management technology, - An urban atmospheric dispersion model and decision-aiding model have been developed. - The technologies for assessing the radiation impact to non-human biota and the environmental medium contamination have developed. - The analytical techniques of the indicator radionuclides related to decommissioning of nuclear facilities and nuclear waste repository have been developed. - The national environmental radiation impact has been assessed, and the optimum management system of natural radiation has been established

  12. Photochemical degradation of chromophoric-dissolved organic matter exposed to simulated UV-B and natural solar radiation

    NARCIS (Netherlands)

    Zhang, Y.; Liu, M.; Qin, B.; Feng, S.

    2009-01-01

    Photochemical degradation of chromophoric-dissolved organic matter (CDOM) by UV-B radiation decreases CDOM absorption in the UV region and fluorescence intensity, and alters CDOM composition. CDOM absorption, fluorescence, and the spectral slope indicating the CDOM composition were studied using

  13. Enhanced UV-B radiation alleviates the adverse effects of summer drought in two Mediterranean pines under field conditions [ozone depletion

    International Nuclear Information System (INIS)

    Petropoulou, Y.; Kyparissis, A.; Nikolopoulos, D.; Manetas, Y.

    1995-01-01

    The effects of enhanced UV-B (290-320 nm) radiation on two native Mediterranean pines (Pinus pinea L., Pinus halepensis Mill.) were recorded during a one-year field study. Plants received ambient or ambient plus supplemental UV-B radiation (simulating a 15% stratospheric ozone depletion over Patras. Greece, 38.3°N. 29.1°E) and only natural precipitation, i.e. they were simultaneously exposed to other natural stresses. particularly water stress during summer. Supplemental UV-B irradiation started in early February, 1993 and up to late June, no effects were observed on growth and photochemical efficiency of photosystem II, as measured by chlorophy II fluorescence induction. Water stress during the summer was manifested in the control plants as a decline in the ratio of variable to maximum fluorescence (F v /F m ), the apparent photon yield for oxygen evolution (φ I ) and the photosynthetic capacity at 5% CO 2 (P m ). In addition, a partial needle loss was evident. Under supplemental UV-B radiation, however, the decreases in F v /F m , φ i , and P m . as well as needle losses were significantly less. Soon after the first heavy autumn rains. photosynthetic parameters in both control and UV-B treated plants recovered to similar values. but the transient summer superiority of UV-B irradiated plants resulted in a significant increase in their dry weight measured at plant harvest. during late January. 1994. Plant height. UV-B absorbing compounds, photosynthetic pigments and relative water content measured at late spring. late summer and at plant harvest, were not significantly affected by supplemental UV-B radiation. The results indicate that enhanced UV-B radiation may be beneficial for Mediterranean pines through a partial alleviation of the adverse effects of summer drought. (author)

  14. Growth, photosynthesis and UV-B absorbing compounds of Portuguese Barbela wheat exposed to ultraviolet-B radiation

    International Nuclear Information System (INIS)

    Correia, C.M.; Torres-Pereira, M.S.; Torres-Pereira, J.M.G.

    1999-01-01

    Wheat plants (Triticum aestivum L.) were exposed to two levels of UV-B radiation (ambient UV-B and high UV-B, simulating a 20% reduction in the ozone layer) under mediterranean field-growth conditions. After 4 months of UV-B treatment, total plant biomass of high UV-B plants was 18% lower compared to control plants. The decrease of biomass appears to be the result of changes in morphological and physiological processes. High UV-B treatment induces decreases in leaf area, net photosynthesis rate, transpiration rate and water use efficiency. Pigment analysis of leaf extracts showed increases in chlorophyll content and no effect on accumulation of UV-B absorbing pigments. The underlying mechanisms for these results are discussed. (author)

  15. ERMS - Environmental Radiation Monitoring System

    International Nuclear Information System (INIS)

    Vax, Eran; Sarusi, Benny; Sheinfeld, Mati; Levinson, Shmuel; Brandys, Irad; Sattinger, Danny; Wengrowicz, Udi; Tshuva, Avi; Tirosh, Dan

    2008-01-01

    A new Environmental Radiation Monitoring System (ERMS) has been developed in the NRCN as an extensive tool to be applied in case of nuclear malfunction or Nuclear Disposal Device (NDD) incident, as well as for routine radiation monitoring of the reactor's vicinity. The system collects real-time environmental data such as: gamma radiation, wind speed, wind direction, and temperature for monitoring purposes. The ERMS consists of a main Control Center and an array of monitoring stations. Fixed, environmental, gamma radiation monitoring stations are installed at the reactor's surroundings while portable stations can be posted rapidly along the wind direction, enhancing the spatial sampling of the radiation measurements and providing better hazard assessment at an emergency event. The presented ERMS, based on industrial standards for hardware and network protocols, is a reliable standalone system which upgrades the readiness to face a nuclear emergency event by supplying real-time, integrated meteorological and radiation data. (author)

  16. Analysis of changes in environmental radiation, and three types of environmental radiation detector performance comparisons

    International Nuclear Information System (INIS)

    Park, J.H; Seo, J.H; Park, S.M; Yu, B.N; Park, J.H; Joo, K.S

    2013-06-01

    High-pressure ion chamber (GE Reuter-Stokes, HPIC), accuracy is high but the high price and do not have the ability nuclide analysis is a disadvantage. NaI(Tl) and PMT scintillation detector of radioactive materials can be divided. Environmental radiation measurements using a semiconductor with SiPM detector PMT to replace the value of the results were compared. SiPM detector using radiation environment were measured in the field to verify the accuracy and energy resolution. SiPMs performance as environmental radiation measurement equipment and radioactive material distinction as a personal dosimeter based technology, using the above results were prepared. The interest on the environmental radiation due to the Fukushima power plant crisis in Japan has been growing concern about the radiation environment of the relatively close proximity Korea is a very heightened state. Could be confirmed in the radiation environment of nuclear power plants around the analysis and performance of the next generation of environmental radiation meter. Fukushima power plants accident after 2 years, the equipment installed by this analysis meets the performance as a radiation detector could be confirmed as follows. CANA Inc. developed by radionuclides classification of using man-made and natural radionuclides and man-made radionuclides separated, ensure the value of the results were analyzed. Could be and alternative to the conventional detector energy resolution ( 137 CS<15%) and linearity (<15%) to satisfy the performance requirements of the measurement result of environmental radiation detector is considered. SiPM radiation environment changes and HPIC and NaI(TI) scintillation detector installed in Korea of the Fukushima power plant after the accident, radiation environment using a small alternative was to verify the accuracy of the measuring equipment. A big difference in performance as invisible by comparison with the large detector Assay miniaturization rough as a personal

  17. Growth under visible light increases conidia and mucilage production and tolerance to UV-B radiation in the plant pathogenic fungus Colletotrichum acutatum.

    Science.gov (United States)

    de Menezes, Henrique D; Massola, Nelson S; Flint, Stephan D; Silva, Geraldo J; Bachmann, Luciano; Rangel, Drauzio E N; Braga, Gilberto U L

    2015-01-01

    Light conditions can influence fungal development. Some spectral wavebands can induce conidial production, whereas others can kill the conidia, reducing the population size and limiting dispersal. The plant pathogenic fungus Colletotrichum acutatum causes anthracnose in several crops. During the asexual stage on the host plant, Colletototrichum produces acervuli with abundant mucilage-embedded conidia. These conidia are responsible for fungal dispersal and host infection. This study examined the effect of visible light during C. acutatum growth on the production of conidia and mucilage and also on the UV tolerance of these conidia. Conidial tolerance to an environmentally realistic UV irradiance was determined both in conidia surrounded by mucilage on sporulating colonies and in conidial suspension. Exposures to visible light during fungal growth increased production of conidia and mucilage as well as conidial tolerance to UV. Colonies exposed to light produced 1.7 times more conidia than colonies grown in continuous darkness. The UV tolerances of conidia produced under light were at least two times higher than conidia produced in the dark. Conidia embedded in the mucilage on sporulating colonies were more tolerant of UV than conidia in suspension that were washed free of mucilage. Conidial tolerance to UV radiation varied among five selected isolates. © 2014 The American Society of Photobiology.

  18. Environmental radiation monitoring around the nuclear facilities

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Chang Woo; Choi, Geun Sik and others

    2001-02-01

    Environmental Radiation Monitoring was carried out with measurement of environment. Radiation and environmental radioactivity analysis around KAERI nuclear facilities and Seoul Research Reactor. The results of environmental radiation monitoring around KAERI nuclear facilities and Seoul Research Reactor are the follows : The average level of environmental radiation dose measured by NaI scintillation counter and accumulated radiation dose by TLD was almost same level compared with the past years. Gross {alpha}, {beta} radioactivity in environmental samples showed a environmental level. {gamma}-radionuclides in water samples were not detected. But only radionuclide K-40, which is natural radionuclide, was detected in the all samples and Cs-137 was detected in the surface soil and discharge sediment. The average level of environmental radiation dose around Seoul Research Reactor was almost same level compared with the past years, and Be-7 and Cs-137 were detected in some surface soil and discharge sediment by {gamma}-spectrometry.

  19. Environmental radiation monitoring around the nuclear facilities

    International Nuclear Information System (INIS)

    Lee, Chang Woo; Choi, Young Ho

    2000-02-01

    Environmental radiation monitoring was carried out with measurement of environment radiation and environmental radioactivity analysis around KAERI nuclear facilities and Seoul Research Reactor. The results of environmental radiation monitoring around KAERI nuclear facilities and Seoul Research Reactor are the follows: The average level of environmental radiation dose measured by NaI scintillation counter and accumulated radiation dose by TLD was almost some level compared with the past years. Gross α, β radioactivity in environmental samples showed a environmental level. γ-radionuclides in water sample were not detected. But only radionuclide K-40, which is natural radionuclide, was detected in the all samples and Cs-137 was detected in the surface soil and discharge sediment. The average level of environmental radiation dose around Seoul Research Reactor was almost same level compared with the past years, and Be-7 and Cs-137 were detected in some surface soil and discharge sediment by γ-spectrometry. (author)

  20. Environmental radiation monitoring around the nuclear facilities

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Chang Woo; Choi, Young Ho

    2000-02-01

    Environmental radiation monitoring was carried out with measurement of environment radiation and environmental radioactivity analysis around KAERI nuclear facilities and Seoul Research Reactor. The results of environmental radiation monitoring around KAERI nuclear facilities and Seoul Research Reactor are the follows: The average level of environmental radiation dose measured by NaI scintillation counter and accumulated radiation dose by TLD was almost some level compared with the past years. Gross {alpha}, {beta} radioactivity in environmental samples showed a environmental level. {gamma}-radionuclides in water sample were not detected. But only radionuclide K-40, which is natural radionuclide, was detected in the all samples and Cs-137 was detected in the surface soil and discharge sediment. The average level of environmental radiation dose around Seoul Research Reactor was almost same level compared with the past years, and Be-7 and Cs-137 were detected in some surface soil and discharge sediment by {gamma}-spectrometry. (author)

  1. Environmental radiation monitoring around the nuclear facilities

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Chang Woo; Choi, Young Ho; Lee, M.H. [and others

    1999-02-01

    Environmental radiation monitoring was carried out with measurement of environment radiation and environmental radioactivity analysis around KAERI nuclear facilities and Seoul research reactor. The results of environmental radiation monitoring around KAERI nuclear facilities and Seoul research reactor are the follows : The average level of environmental radiation dose measured by NaI scintillation counter and accumulated radiation dose by TLD was almost same level compared with the past years. Gross {alpha}, {beta} radioactivity in environmental samples showed a environmental level. {gamma}-radionuclides in water samples were not detected. But only radionuclide K-40, which is natural radionuclide, was detected in the all samples and Cs-137 was detected in the surface soil and discharge sediment. The average level of environmental radiation dose around Seoul research reactor was almost same level compared with the past years, and Be-7 and Cs-137 were detected in some surface soil and discharge sediment by {gamma}-spectrometry. (author). 3 refs., 50 tabs., 12 figs.

  2. Environmental radiation monitoring around the nuclear facilities

    International Nuclear Information System (INIS)

    Lee, Chang Woo; Choi, Geun Sik and others

    2001-02-01

    Environmental Radiation Monitoring was carried out with measurement of environment. Radiation and environmental radioactivity analysis around KAERI nuclear facilities and Seoul Research Reactor. The results of environmental radiation monitoring around KAERI nuclear facilities and Seoul Research Reactor are the follows : The average level of environmental radiation dose measured by NaI scintillation counter and accumulated radiation dose by TLD was almost same level compared with the past years. Gross α, β radioactivity in environmental samples showed a environmental level. γ-radionuclides in water samples were not detected. But only radionuclide K-40, which is natural radionuclide, was detected in the all samples and Cs-137 was detected in the surface soil and discharge sediment. The average level of environmental radiation dose around Seoul Research Reactor was almost same level compared with the past years, and Be-7 and Cs-137 were detected in some surface soil and discharge sediment by γ-spectrometry

  3. Environmental radiation monitoring around the research reactors

    International Nuclear Information System (INIS)

    Lee, Chang Woo; Lee, Hyun Duk; Kim, Sam Rang; Choi, Yong Ho; Kim, Jeong Moo; Lee, Myeon Joo; Lee, Myeong Ho; Hong, Kwang Hee; Lim, Moon Ho; Lee, Won Yoon; Park, Do Won; Choi, Sang Do

    1993-04-01

    Environmental radiation monitoring was carried out measurement of environmental radiation and environmental radioactivity analysis around KAERI nuclear facilities and Seoul research reactor. The results of environmental radiation monitoring around KAERI nuclear facilities and Seoul research reactor are the follows : The average level of environmental radiation dose measured by NaI scintillation counter and accumulated radiation dose by TLD was almost same level compared with the past years. Gross α, β radioactivity in environmental sample was not found abnormal data. γ-radionuclides in water samples were not detected. But only radionuclide K-40, which is natural radionuclide, was detected in the all samples and Cs-137 was detected in the surface soil and discharge sediment. The average level of environmental radiation dose around Seoul research reactor was almost same level compared with the past years, and Be-7 and Cs-137 were detected in some surface soil and discharge sediment by γ-spectrometry. (Author)

  4. Inactivation credit of UV radiation for viruses, bacteria and protozoan (oo)cysts in water: a review

    NARCIS (Netherlands)

    Hijnen, W.A.M.; Beerendonk, E.F.; Medema, Gerriet Jan

    2006-01-01

    UV disinfection technology is of growing interest in the water industry since it was demonstrated that UV radiation is very effective against (oo)cysts of Cryptosporidium and Giardia, two pathogenic micro-organisms of major importance for the safety of drinking water. Quantitative Microbial Risk

  5. [Knowledge about UV-radiation and sun protection: survey of adolescents and young adults in Bavaria].

    Science.gov (United States)

    Eichhorn, C; Seibold, C; Loss, J; Steinmann, A; Nagel, E

    2008-10-01

    Identifying deficits in sun protection knowledge and behavior can serve as a starting point for primary prevention interventions. The aim of this study was to investigate knowledge and behavior related to ultraviolet radiation in the population between 14 and 45 years of age in Bavaria, as well as effects of the awareness campaign "Sensible in the Sun". In two Bavarian districts, 545 individuals of the target population completed a telephone survey about risks of UV-radiation, sun protection knowledge and behavior, and effects of the campaign. Sunburn and skin cancer as adverse effects of ultraviolet radiation were named by almost every participant. When asked about protective interventions, 91% mentioned sunscreen and 45-54% clothing, limited stay in the sun and seeking shade at noon. Women were better informed than men, adults better than adolescents. 10.6% were aware of the campaign. In this group, 37.9% had been motivated to consider their sun protective behavior; 13.8% (especially women >30 years) stated they had changed their behavior because of the campaign. There were deficits in knowledge, especially about eye damage and the importance of getting slowly used to UV radiation. Physician advice, but also broadcast and print media, has an effect on UV-related knowledge.

  6. Environmental radiation standards

    International Nuclear Information System (INIS)

    Kocher, D.C.

    1987-01-01

    This document contains an outline of an oral presentation on environmental radiation standards presented to the American Nuclear Societies' Topical Conference on Population Exposure from the Nuclear Fuel Cycle. The paper contains several definitions, a summary of current radiation exposure limits; and numerous proposed changes to current standards. 7 figs

  7. UV disinfection of water. 1. Effect on microorganisms/virus conditions which can limit the use of UV radiation as a means of disinfection

    Energy Technology Data Exchange (ETDEWEB)

    Skipperud, E; Johansen,; Myhrstad, J A [Statens Inst. for Folkehelse, Oslo (Norway)

    1978-01-01

    UV radiation has been found to have advantages over chloration for the disinfection of water. New regulations for dietary conditions on Norwegian ships introduced in 1974 led to increased use of UV disinfection, and this has in the following years spread to waterworks. The present article is based on a study to determine possible limitation. The nature of the injuries to the microorganisms is first discussed, together with repair mechanisms. A table is given showing the energy required for 90 and 100 percent inactivation of a number of microorganisms. Some other factors affecting UV inactivation are briefly mentioned. (JIW).

  8. Reproductive, morphological, and phytochemical responses of Arabidopsis thaliana ecotypes to enhanced UV-B radiation

    Energy Technology Data Exchange (ETDEWEB)

    Trumbull, V.L.; McCloud, E.S.; Paige, K.N. (Univ. of Illinois, Urbana, IL (United States))

    1994-06-01

    Two ecotypes of Arabidopsis thaliana, collected from Libya and Norway, were grown in the greenhouse under. UV-B doses of 0 and 10.5 kJ m[sup [minus]2] UV-B[sub BE]. The high UV-B dose simulated midsummer ambient conditions over Libya and a 40% reduction in stratospheric ozone over Norway. The Libyan ectotype, which originated from latitudes where solar UV-B is high, showed no UV-B induced damage to plant growth. However the Norwegian ecotype, which originated from latitudes where solar UV-B is low, showed a significant reduction in plant height, inflorescence weight, and rosette weight in response to enhanced UV-B. Although fruit and seed number for both ecotypes were unaffected by enhanced UV-B radiation the germination success of the seeds harvested from the irradiated Norwegian plants were significantly reduced. The two ecotypes also differed with respect to their accumulation of kaempferol, a putative UV-B protective filter. The Libyan ecotype increased kaempferol concentration by 38% over the 0 kJ treatment whereas the Norwegian ecotype increased by only 15%. These data suggest that, for these ecotypes, variation in UV-B sensitivity may be explained by the differential induction of UV-absorbing leaf pigments.

  9. Response of oxidative stress defense systems in rice (Oryza sativa) leaves with supplemental UV-B radiation

    International Nuclear Information System (INIS)

    Dai, Q.; Yan, B.; Huang, S.; Liu, X.; Peng, S.; Miranda, M.L.L.; Chavez, A.Q.; Vergara, B.S.; Olszyk, D.M.

    1997-01-01

    The impact of elevated ultraviolet-B radiation (UV-B, 280–320 nm) on membrane systems and lipid peroxidation, and possible involvement of active oxygen radicals was investigated in leaves of two UV-B susceptible rice cultivars (Oryza sativa L. cvs IR74 and Dular). Rice seedlings were grown in a greenhouse for 10 days and then treated with biologically effective UV-B (UV-B BE ) radiation for 28 days. Oxidative stress effects were evaluated by measuring superoxide anion (O 2 ) generation rate, hydrogen peroxide (H 2 O 2 ) content, malondialdehyde (MDA) concentration and relative electrolyte conductivity (EC) for IR74 and Dular at 0 (control), 6 or 13 kJ m −2 day −1 UV-B BE . Significant increases in these parameters were found in rice plants grown at 13 vs 0 kJ m −2 day −1 UV-B BE after 28 days; indicating that disruption of membrane systems may be an eventual reason for UV-B-induced injury in rice plants. There was a positive correlation between O 2 − generation and increases in EC or MDA in leaves. Activities of enzymatic and nonenzymatic free radical scavengers were measured for IR74 after 7, 14, 21 and 28 days of exposure to 13 or 0 UV-B BE to evaluate dynamics of these responses over time. Activities of catalase and superoxide dismutase (but not ascorbate peroxidase) and concentrations of ascorbic acid and glutathione were enhanced by 13 vs 0 UV-B BE after 14 days of UV-B exposure. Further exposure to 28 days of UV-B was associated with a decline in enzyme activities and ascorbic acid, but not glutathione. It is suggested that UV-B-induced injury may be associated with disturbance of active oxygen metabolism through the destruction and alteration of both enzymatic and nonenzymatic defense systems in rice. (author)

  10. Bactericidal effectiveness of UV radiators, type DRT-400, in a broilerhouse

    International Nuclear Information System (INIS)

    Dobrzanski, Z.; Al Faouri, W.; Markiewicz, M.; Pawiak, R.

    1989-01-01

    Three UV radiators, type DRT-400, were used in a broilerhouse of BIOS type; 12 lamps in a set, type ZNUV, worked inside of the premises for 5, 8, 12, 16 and 20 min. It was found a high degree reduction of bacteria and fungi in the air, at 60.3 and 74.0% respectively, on the external surface of bedding at 40.3% and 72.2%, and in automatic watering troughs at 44.0 and 97.7% respectively. The degree of microorganisms reduction depended on the time exposition to UV, general air contamination, place of contamination, i.e. bedding or water in troughs. (author)

  11. UV Radiation Detection Using Optical Sensor Based on Eu3+ Doped PMMA

    Directory of Open Access Journals (Sweden)

    Miluski Piotr

    2016-12-01

    Full Text Available Progress in UV treatment applications requires new compact and sensor constructions. In the paper a hybrid (organic-inorganic rare-earth-based polymeric UV sensor construction is proposed. The efficient luminescence of poly(methyl methacrylate (PMMA matrix doped by europium was used for testing the optical sensor (optrode construction. The europium complex assures effective luminescence in the visible range with well determined multi-peak spectrum emission enabling construction of the optrode. The fabricated UV optical fibre sensor was used for determination of Nd:YAG laser intensity measurements at the third harmonic (355 nm in the radiation power range 5.0-34.0 mW. The multi-peak luminescence spectrum was used for optimization of the measurement formula. The composition of luminescent peak intensity enables to increase the slope of sensitivity up to −2.8 mW-1. The obtained results and advantages of the optical fibre construction enable to apply it in numerous UV detection systems.

  12. Environmental radiation monitoring from the decommission of TRIGA

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Geun Sik; Lee, Chang Woo

    2000-03-01

    Environmental radiation monitoring was carried out with measurement of environmental radiation and environmental radioactivity analysis around TRIGA Research Reactor. The results of environmental radiation monitoring around TRIGA Research Reactor are the follows: The average level of environmental radiation measured by potable ERM and accumulated radiation dose by TLD was almost same level compared with thepast years. Gross {beta} radioactivity in environmental samples showed a environmental level. {gamma}-radionuclides in water samples were not detected. but only radionuclide K-40, which is natural radionuclide, was detected in the all samples and Cs-137 was detected in the surface soil and discharge sediment. (author)

  13. UV-Induced Cell Death in Plants

    Science.gov (United States)

    Nawkar, Ganesh M.; Maibam, Punyakishore; Park, Jung Hoon; Sahi, Vaidurya Pratap; Lee, Sang Yeol; Kang, Chang Ho

    2013-01-01

    Plants are photosynthetic organisms that depend on sunlight for energy. Plants respond to light through different photoreceptors and show photomorphogenic development. Apart from Photosynthetically Active Radiation (PAR; 400–700 nm), plants are exposed to UV light, which is comprised of UV-C (below 280 nm), UV-B (280–320 nm) and UV-A (320–390 nm). The atmospheric ozone layer protects UV-C radiation from reaching earth while the UVR8 protein acts as a receptor for UV-B radiation. Low levels of UV-B exposure initiate signaling through UVR8 and induce secondary metabolite genes involved in protection against UV while higher dosages are very detrimental to plants. It has also been reported that genes involved in MAPK cascade help the plant in providing tolerance against UV radiation. The important targets of UV radiation in plant cells are DNA, lipids and proteins and also vital processes such as photosynthesis. Recent studies showed that, in response to UV radiation, mitochondria and chloroplasts produce a reactive oxygen species (ROS). Arabidopsis metacaspase-8 (AtMC8) is induced in response to oxidative stress caused by ROS, which acts downstream of the radical induced cell death (AtRCD1) gene making plants vulnerable to cell death. The studies on salicylic and jasmonic acid signaling mutants revealed that SA and JA regulate the ROS level and antagonize ROS mediated cell death. Recently, molecular studies have revealed genes involved in response to UV exposure, with respect to programmed cell death (PCD). PMID:23344059

  14. Anthropogenic changes in the surface all-sky UV-B radiation through 1850-2005 simulated by an Earth system model

    Science.gov (United States)

    Watanabe, S.; Takemura, T.; Sudo, K.; Yokohata, T.; Kawase, H.

    2012-06-01

    The historical anthropogenic change in the surface all-sky UV-B (solar ultraviolet: 280-315 nm) radiation through 1850-2005 is evaluated using an Earth system model. Responses of UV-B dose to anthropogenic changes in ozone and aerosols are separately evaluated using a series of historical simulations including/excluding these changes. Increases in these air pollutants cause reductions in UV-B transmittance, which occur gradually/rapidly before/after 1950 in and downwind of industrial and deforestation regions. Furthermore, changes in ozone transport in the lower stratosphere, which is induced by increasing greenhouse gas concentrations, increase ozone concentration in the extratropical upper troposphere and lower stratosphere. These transient changes work to decrease the amount of UV-B reaching the Earth's surface, counteracting the well-known effect increasing UV-B due to stratospheric ozone depletion, which developed rapidly after ca. 1980. As a consequence, the surface UV-B radiation change between 1850 and 2000 is negative in the tropics and NH extratropics and positive in the SH extratropics. Comparing the contributions of ozone and aerosol changes to the UV-B change, the transient change in ozone absorption of UV-B mainly determines the total change in the surface UV-B radiation at most locations. On the other hand, the aerosol direct and indirect effects on UV-B play an equally important role to that of ozone in the NH mid-latitudes and tropics. A typical example is East Asia (25° N-60° N and 120° E-150° E), where the effect of aerosols (ca. 70%) dominates the total UV-B change.

  15. UV radiation: a promising tool in the synthesisof multicomponent nano-oxides

    Czech Academy of Sciences Publication Activity Database

    Čuba, V.; Procházková, L.; Bárta, J.; Vondrášková, A.; Pavelková, T.; Mihóková, Eva; Jarý, Vítězslav; Nikl, Martin

    2014-01-01

    Roč. 16, č. 11 (2014), "2686-1"-"2686-7" ISSN 1388-0764 R&D Projects: GA ČR GA13-09876S Institutional support: RVO:68378271 Keywords : nanoscintillators * band-gap engineering * UV radiation radioluminescence * zinc oxide * synthetic garnets * composite nanoparticles Subject RIV: CH - Nuclear ; Quantum Chemistry Impact factor: 2.184, year: 2014

  16. Dose/dose-rate responses of shrimp larvae to UV-B radiation

    International Nuclear Information System (INIS)

    Damkaer, D.M.

    1981-01-01

    Previous work indicated dose-rate thresholds in the effects of UV-B on the near-surface larvae of three shrimp species. Additional observations suggest that the total dose response varies with dose-rate. Below 0.002 Wm -2 sub([DNA]) irradiance no significant effect is noted in activity, development, or survival. Beyond that dose-rate threshold, shrimp larvae are significantly affected if the total dose exceeds about 85 Jm -2 sub([DNA]). Predictions cannot be made without both the dose-rate and the dose. These dose/dose-rate thresholds are compared to four-year mean dose/dose-rate solar UV-B irradiances at the experimental site, measured at the surface and calculated for 1 m depth. The probability that the shrimp larvae would receive lethal irradiance is low for the first half of the season of surface occurrence, even with a 44% increase in damaging UV radiation. (orig.)

  17. Dose/dose-rate responses of shrimp larvae to UV-B radiation

    Energy Technology Data Exchange (ETDEWEB)

    Damkaer, D.M.; Dey, D.B.; Heron, G.A.

    1981-01-01

    Previous work indicated dose-rate thresholds in the effects of UV-B on the near-surface larvae of three shrimp species. Additional observations suggest that the total dose response varies with dose-rate. Below 0.002 Wm/sup -2/sub((DNA)) irradiance no significant effect is noted in activity, development, or survival. Beyond that dose-rate threshold, shrimp larvae are significantly affected if the total dose exceeds about 85 Jm/sup -2/sub((DNA)). Predictions cannot be made without both the dose-rate and the dose. These dose/dose-rate thresholds are compared to four-year mean dose/dose-rate solar UV-B irradiances at the experimental site, measured at the surface and calculated for 1 m depth. The probability that the shrimp larvae would receive lethal irradiance is low for the first half of the season of surface occurrence, even with a 44% increase in damaging UV radiation.

  18. Optimizing UV Index determination from broadband irradiances

    Science.gov (United States)

    Tereszchuk, Keith A.; Rochon, Yves J.; McLinden, Chris A.; Vaillancourt, Paul A.

    2018-03-01

    A study was undertaken to improve upon the prognosticative capability of Environment and Climate Change Canada's (ECCC) UV Index forecast model. An aspect of that work, and the topic of this communication, was to investigate the use of the four UV broadband surface irradiance fields generated by ECCC's Global Environmental Multiscale (GEM) numerical prediction model to determine the UV Index. The basis of the investigation involves the creation of a suite of routines which employ high-spectral-resolution radiative transfer code developed to calculate UV Index fields from GEM forecasts. These routines employ a modified version of the Cloud-J v7.4 radiative transfer model, which integrates GEM output to produce high-spectral-resolution surface irradiance fields. The output generated using the high-resolution radiative transfer code served to verify and calibrate GEM broadband surface irradiances under clear-sky conditions and their use in providing the UV Index. A subsequent comparison of irradiances and UV Index under cloudy conditions was also performed. Linear correlation agreement of surface irradiances from the two models for each of the two higher UV bands covering 310.70-330.0 and 330.03-400.00 nm is typically greater than 95 % for clear-sky conditions with associated root-mean-square relative errors of 6.4 and 4.0 %. However, underestimations of clear-sky GEM irradiances were found on the order of ˜ 30-50 % for the 294.12-310.70 nm band and by a factor of ˜ 30 for the 280.11-294.12 nm band. This underestimation can be significant for UV Index determination but would not impact weather forecasting. Corresponding empirical adjustments were applied to the broadband irradiances now giving a correlation coefficient of unity. From these, a least-squares fitting was derived for the calculation of the UV Index. The resultant differences in UV indices from the high-spectral-resolution irradiances and the resultant GEM broadband irradiances are typically within 0

  19. Effects of Stratospheric Ozone Depletion, Solar UV Radiation, and Climate Change on Biogeochemical Cycling: Interactions and Feedbacks

    Science.gov (United States)

    Climate change modulates the effects of solar UV radiation on biogeochemical cycles in terrestrial and aquatic ecosystems, particularly for carbon cycling, resulting in UV-mediated positive or negative feedbacks on climate. Possible positive feedbacks discussed in this assessment...

  20. Row orientation effect on UV-B, UV-A and PAR solar irradiation components in vineyards at Tuscany, Italy

    Science.gov (United States)

    Grifoni, D.; Carreras, G.; Zipoli, G.; Sabatini, F.; Dalla Marta, A.; Orlandini, S.

    2008-11-01

    Besides playing an essential role in plant photosynthesis, solar radiation is also involved in many other important biological processes. In particular, it has been demonstrated that ultraviolet (UV) solar radiation plays a relevant role in grapevines ( Vitis vinifera) in the production of certain important chemical compounds directly responsible for yield and wine quality. Moreover, the exposure to UV-B radiation (280-320 nm) can affect plant-disease interaction by influencing the behaviour of both pathogen and host. The main objective of this research was to characterise the solar radiative regime of a vineyard, in terms of photosynthetically active radiation (PAR) and UV components. In this analysis, solar spectral UV irradiance components, broadband UV (280-400 nm), spectral UV-B and UV-A (320-400 nm), the biological effective UVBE, as well as the PAR (400-700 nm) component, were all considered. The diurnal patterns of these quantities and the UV-B/PAR and UV-B/UV-A ratios were analysed to investigate the effect of row orientation of the vineyard in combination with solar azimuth and elevation angles. The distribution of PAR and UV irradiance at various heights of the vertical sides of the rows was also studied. The results showed that the highest portion of plants received higher levels of daily radiation, especially the UV-B component. Row orientation of the vines had a pronounced effect on the global PAR received by the two sides of the rows and, to a lesser extent, UV-A and UV-B. When only the diffused component was considered, this geometrical effect was greatly attenuated. UV-B/PAR and UV-A/PAR ratios were also affected, with potential consequences on physiological processes. Because of the high diffusive capacity of the UV-B radiation, the UV-B/PAR ratio was significantly lower on the plant portions exposed to full sunlight than on those in the shade.

  1. Radiation environmental impact assessment of copper exploitation

    International Nuclear Information System (INIS)

    Fan Guang; Wen Zhijian

    2010-01-01

    The radiation environmental impact of mineral exploitation on the surrounding environment has become a public concern. This paper presents the radiation environmental impact assessment of copper exploitation. Based on the project description and detailed investigations of surrounding environment, systematic radiation environmental impacts have been identified. The environmental impacts are assessed during both construction and operation phase. The environmental protection measures have also been proposed. The related conclusion and measures can play an active role in copper exploitation and environmental protection. (authors)

  2. Effect of solar radiation (UV and visible) at high altitude on CAM-cycling and phenolic compound biosynthesis in Sedum album

    International Nuclear Information System (INIS)

    Bachereau, F.; Marigo, G.; Asta, J.

    1998-01-01

    The field experiment was carried out in order to compare the response of a CAM plant, Sedum album L., to solar radiation at a high altitude (2 100 m) with that at a low altitude location with respect to CAM and phenolic content. Treatment sites included (1) sun-exposed, low altitude, (2) sun-exposed, high altitude with different light treatments, including UV-B and UV-B + A screening, and (3) shade at high altitude. After a 70-day treatment period, CAM-cycling and phenolic compound content were analysed, and high altitude treatments were compared to the low altitude control. The sun-exposed low altitude control was characterized by CAM-cycling and a low phenolic compound content during the experiment. In plants transplanted to the high altitude, only the shaded group maintained a CAM-cycling and a phenolic compound content similar to those of the sun-exposed low altitude control. Samples under UV-B and UV-B + A filters showed similar responses, suggesting the absence of a specific UV-A radiation effect. The screening of UV-B or UV-B + A radiation allowed plants to partially maintain a CAM-cycling and induced a decrease in phenolic compound content. These responses under UV filters were, however, intermediate between those observed in sun-exposed and shaded groups. These results demonstrate a specific effect of radiation from both visible (400–800 nm) and UV-B (280–320 nm) bands on both CAM-cycling and phenolic biosynthesis in S. album L. plants. These light-dependent effects are discussed on a physiological basis and a possible interaction between CAM-cycling and phenolic metabolism is suggested. (author)

  3. Development of Ultraviolet (UV) Radiation Protective Fabric Using Combined Electrospinning and Electrospraying Technique

    Science.gov (United States)

    Sinha, Mukesh Kumar; Das, B. R.; Kumar, Kamal; Kishore, Brij; Prasad, N. Eswara

    2017-06-01

    The article reports a novel technique for functionization of nanoweb to develop ultraviolet (UV) radiation protective fabric. UV radiation protection effect is produced by combination of electrospinning and electrospraying technique. A nanofibrous web of polyvinylidene difluoride (PVDF) coated on polypropylene nonwoven fabric is produced by latest nanospider technology. Subsequently, web is functionalized by titanium dioxide (TiO2). The developed web is characterized for evaluation of surface morphology and other functional properties; mechanical, chemical, crystalline and thermal. An optimal (judicious) nanofibre spinning condition is achieved and established. The produced web is uniformly coated by defect free functional nanofibres in a continuous form of useable textile structural membrane for ultraviolet (UV) protective clothing. This research initiative succeeds in preparation and optimization of various nanowebs for UV protection. Field Emission Scanning Electron Microscope (FESEM) result reveals that PVDF webs photo-degradative behavior is non-accelerated, as compared to normal polymeric grade fibres. Functionalization with TiO2 has enhanced the photo-stability of webs. The ultraviolet protection factor of functionalized and non-functionalized nanowebs empirically evaluated to be 65 and 24 respectively. The developed coated layer could be exploited for developing various defence, para-military and civilian UV protective light weight clothing (tent, covers and shelter segments, combat suit, snow bound camouflaging nets). This research therefore, is conducted in an attempt to develop a scientific understanding of PVDF fibre coated webs for photo-degradation and applications for defence protective textiles. This technological research in laboratory scale could be translated into bulk productionization.

  4. Diurnal changes in epidermal UV transmittance of plants in naturally high UV environments.

    Science.gov (United States)

    Barnes, Paul W; Flint, Stephan D; Slusser, James R; Gao, Wei; Ryel, Ronald J

    2008-06-01

    Studies were conducted on three herbaceous plant species growing in naturally high solar UV environments in the subalpine of Mauna Kea, Hawaii, USA, to determine if diurnal changes in epidermal UV transmittance (T(UV)) occur in these species, and to test whether manipulation of the solar radiation regime could alter these diurnal patterns. Additional field studies were conducted at Logan, Utah, USA, to determine if solar UV was causing diurnal T(UV) changes and to evaluate the relationship between diurnal changes in T(UV) and UV-absorbing pigments. Under clear skies, T(UV), as measured with a UV-A-pulse amplitude modulation fluorometer for leaves of Verbascum thapsus and Oenothera stricta growing in native soils and Vicia faba growing in pots, was highest at predawn and sunset and lowest at midday. These patterns in T(UV) closely tracked diurnal changes in solar radiation and were the result of correlated changes in fluorescence induced by UV-A and blue radiation but not photochemical efficiency (F(v)/F(m)) or initial fluorescence yield (F(o)). The magnitude of the midday reduction in T(UV) was greater for young leaves than for older leaves of Verbascum. Imposition of artificial shade eliminated the diurnal changes in T(UV) in Verbascum, but reduction in solar UV had no effect on diurnal T(UV) changes in Vicia. In Vicia, the diurnal changes in T(UV) occurred without detectable changes in the concentration of whole-leaf UV-absorbing compounds. Results suggest that plants actively control diurnal changes in UV shielding, and these changes occur in response to signals other than solar UV; however, the underlying mechanisms responsible for rapid changes in T(UV) remain unclear.

  5. In vivo effects of UV radiation on multiple endpoints and expression profiles of DNA repair and heat shock protein (Hsp) genes in the cycloid copepod Paracyclopina nana

    International Nuclear Information System (INIS)

    Won, Eun-Ji; Han, Jeonghoon; Lee, Yeonjung; Kumar, K. Suresh; Shin, Kyung-Hoon; Lee, Su-Jae; Park, Heum Gi; Lee, Jae-Seong

    2015-01-01

    Highlights: • UV-B radiation induced a significant reduction of the re-brooding rate of ovigerous females. • A dose-dependent decrease in food ingestion and the rate of assimilation to the body upon UV radiation. • Expression of base excision repair-associated and hsp chaperoning genes was significantly increased upon UV radiation in P. nana. - Abstract: To evaluate the effects of ultraviolet (UV) radiation on energy acquisition and consumption, the copepod Paracyclopina nana was irradiated with several doses (0–3 kJ/m 2 ) of UV. After UV radiation, we measured the re-brooding success, growth pattern of newly hatched nauplii, ingestion rate, and assimilation of diet. In addition, we checked the modulated patterns of DNA repair and heat shock protein (hsp) chaperoning genes of P. nana. UV-B radiation induced a significant reduction (7–87%) of the re-brooding rate of ovigerous females, indicating that UV-induced egg sac damage is closely correlated with a reduction in the hatching rate of UV-irradiated ovigerous female offspring. Using chlorophyll a and stable carbon isotope incubation experiments, we found a dose-dependent decrease (P < 0.05) in food ingestion and the rate of assimilation to the body in response to UV radiation, implying that P. nana has an underlying ability to shift its balanced-energy status from growth and reproduction to DNA repair and adaptation. Also, expression of P. nana base excision repair (BER)-associated genes and hsp chaperoning genes was significantly increased in response to UV radiation in P. nana. These findings indicate that even 1 kJ/m 2 of UV radiation induces a reduction in reproduction and growth patterns, alters the physiological balance and inhibits the ability to cope with UV-induced damage in P. nana

  6. In vivo effects of UV radiation on multiple endpoints and expression profiles of DNA repair and heat shock protein (Hsp) genes in the cycloid copepod Paracyclopina nana

    Energy Technology Data Exchange (ETDEWEB)

    Won, Eun-Ji; Han, Jeonghoon [Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Lee, Yeonjung; Kumar, K. Suresh; Shin, Kyung-Hoon [Department of Marine Sciences and Convergent Technology, College of Science and Technology, Hanyang University, Ansan 426-791 (Korea, Republic of); Lee, Su-Jae [Department of Life Sciences, College of Natural Sciences, Hanyang University, Seoul 133-791 (Korea, Republic of); Park, Heum Gi, E-mail: hgpark@gwnu.ac.kr [Department of Marine Resource Development, College of Life Sciences, Gangneung-Wonju National University, Gangneung 210-702 (Korea, Republic of); Lee, Jae-Seong, E-mail: jslee2@skku.edu [Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of)

    2015-08-15

    Highlights: • UV-B radiation induced a significant reduction of the re-brooding rate of ovigerous females. • A dose-dependent decrease in food ingestion and the rate of assimilation to the body upon UV radiation. • Expression of base excision repair-associated and hsp chaperoning genes was significantly increased upon UV radiation in P. nana. - Abstract: To evaluate the effects of ultraviolet (UV) radiation on energy acquisition and consumption, the copepod Paracyclopina nana was irradiated with several doses (0–3 kJ/m{sup 2}) of UV. After UV radiation, we measured the re-brooding success, growth pattern of newly hatched nauplii, ingestion rate, and assimilation of diet. In addition, we checked the modulated patterns of DNA repair and heat shock protein (hsp) chaperoning genes of P. nana. UV-B radiation induced a significant reduction (7–87%) of the re-brooding rate of ovigerous females, indicating that UV-induced egg sac damage is closely correlated with a reduction in the hatching rate of UV-irradiated ovigerous female offspring. Using chlorophyll a and stable carbon isotope incubation experiments, we found a dose-dependent decrease (P < 0.05) in food ingestion and the rate of assimilation to the body in response to UV radiation, implying that P. nana has an underlying ability to shift its balanced-energy status from growth and reproduction to DNA repair and adaptation. Also, expression of P. nana base excision repair (BER)-associated genes and hsp chaperoning genes was significantly increased in response to UV radiation in P. nana. These findings indicate that even 1 kJ/m{sup 2} of UV radiation induces a reduction in reproduction and growth patterns, alters the physiological balance and inhibits the ability to cope with UV-induced damage in P. nana.

  7. Choice of Eye-Safe Radiation Wavelength in UV and Near IR Spectral Bands for Remote Sensing

    Directory of Open Access Journals (Sweden)

    M. L. Belov

    2016-01-01

    Full Text Available The introduction of laser remote sensing systems carries a particular risk to the human’s sense of vision. A structure of the eye, and especially the retina, is the main critical organ as related to the laser radiation.The work uses the optical models of the atmosphere, correctly working in both the UV and the near-IR band, to select the eye-safe radiation wavelengths in the UV (0.355 m and near-IR (~ 1.54 and ~ 2 m spectral bands from the point of view of recorded lidar signal value to fulfill the tasks of laser sensing the natural formations and laser aerosol sensing in the atmosphere.It is shown that the remote sensing lasers with appropriate characteristics can be selected both in the UV band (at a wavelength of 0.355 μm and in the near-IR band (at wavelengths of 1.54 ~ or ~ 2 μm.Molecular scattering has its maximum (for the selected wavelength at a wavelength of 0.355 μm in the UV band, and the minimum at the wavelengths of 1.54 and 2.09 μm in the near -IR band. The main contribution to the molecular absorption at a wavelength of 0.355 μm is made by ozone. In the near-IR spectral band the radiation is absorbed due to water vapor and carbon dioxide.Calculations show that the total effect of the molecular absorption and scattering has no influence on radiation transmission for both the wavelength of 0.355 μm in the UV band, and the wavelengths of 1.54 and 2.09 μm in the near-IR band for sensing trails ~ 1 km.One of the main factors of laser radiation attenuation in the Earth's atmosphere is radiation scattering by aerosol particles.The results of calculations at wavelengths of 0.355 μm, 1.54 μm and 2.09 μm for the several models of the atmosphere show that a choice of the most effective (in terms of the recorded signal of lidar and eye-safe radiation wavelength depends strongly on the task of sensing.To fulfill the task of laser sensing the natural formations, among the eye-safe wavelengths there is one significantly advantageous

  8. Thermoluminescence dependence on the wavelength of monochromatic UV-radiation in Cu-doped KCl and KBr at room temperature

    Energy Technology Data Exchange (ETDEWEB)

    Perez R, A.; Piters, T.; Aceves, R.; Rodriguez M, R.; Perez S, R., E-mail: rperez@cifus.uson.mx [Universidad de Sonora, Departamento de Investigaciones en Fisica, Apdo. Postal 5-088, 83190 Hermosillo, Sonora (Mexico)

    2014-08-15

    Thermoluminescence (Tl) dependence on the UV irradiation wavelengths from 200 to 500 nm in Cu-doped KCl and KBr crystals with different thermal treatment has been analyzed. Spectrum of the Tl intensity of each material show lower intensity at wavelengths longer than 420 nm. The Tl intensity depends on the irradiation wavelength. Structure of the Tl intensity spectrum of each sample is very similar to the structure of its optical absorption spectrum, indicating that at each wavelength, monochromatic radiation is absorbed to produce electronic transitions and electron hole pairs. Thermoluminescence of materials with thermal treatment at high temperature shows electron-hole trapping with less efficiency. The results show that Cu-doped alkali-halide materials are good detectors of a wide range of UV monochromatic radiations and could be used to measure UV radiation doses. (Author)

  9. Thermoluminescence dependence on the wavelength of monochromatic UV-radiation in Cu-doped KCl and KBr at room temperature

    International Nuclear Information System (INIS)

    Perez R, A.; Piters, T.; Aceves, R.; Rodriguez M, R.; Perez S, R.

    2014-08-01

    Thermoluminescence (Tl) dependence on the UV irradiation wavelengths from 200 to 500 nm in Cu-doped KCl and KBr crystals with different thermal treatment has been analyzed. Spectrum of the Tl intensity of each material show lower intensity at wavelengths longer than 420 nm. The Tl intensity depends on the irradiation wavelength. Structure of the Tl intensity spectrum of each sample is very similar to the structure of its optical absorption spectrum, indicating that at each wavelength, monochromatic radiation is absorbed to produce electronic transitions and electron hole pairs. Thermoluminescence of materials with thermal treatment at high temperature shows electron-hole trapping with less efficiency. The results show that Cu-doped alkali-halide materials are good detectors of a wide range of UV monochromatic radiations and could be used to measure UV radiation doses. (Author)

  10. Environmental radiation monitoring system

    International Nuclear Information System (INIS)

    Kato, Tsutomu; Shioiri, Masatoshi; Sakamaki, Tsuyoshi

    2007-01-01

    Environmental radiation monitoring systems are used to measure and monitoring gamma-rays at the observation boundaries of nuclear facilities and in the surrounding areas. In recent years, however, few new nuclear facilities have been constructed and the monitoring systems shift to renewal of existing systems. In addition, in order to increase public acceptance, the facilities are being equipped with communication lines to provide data to prefectural environmental centers. In this text, we introduce the latest technology incorporated in replacement of environmental radiation monitoring systems. We also introduce a replacement method that can shorten the duration during which environmental dose rate measurement is interrupted by enabling both the replacement system and the system being replaced to perform measurements in parallel immediately before and after the replacement. (author)

  11. Repair of radiation damage of Micrococcus radioproteolyticus due to gamma and UV irradiation

    International Nuclear Information System (INIS)

    Ryznar, L.; Drasil, V.

    1982-01-01

    Cells were irradiated in dry state with gamma radiation and UV radiation. The post-irradiation warming of freeze dried cells (2 hours to 60deg or to 80deg) influenced the ability to repair sublethal damage. Heating to 80deg caused a mild reduction in survival. The repair of irradiated and heated cells required more time than that of cells which had only been irradiated. (M.D.)

  12. The role of p-aminobenzoic acid in the repair of injuries induced by UV- and γ-radiation

    International Nuclear Information System (INIS)

    Rapoport, I.A.; Vasil'eva, S.V.; Davnichenko, L.S.

    1979-01-01

    For the first time it was proved that low doses of p-aminobenzoic acid (PABA) were capable of sharply decreasing lethal mutational effects of UV light and less significantly-gamma effect on a bacterial cell. The experiments were carried out on E.Coli strains which differed in the activity of ferment system of DNA UV-induced injuries reparation. VV radiation dose equaled 10-1500 erd/mm 2 . PABA capability to intensify the reparative process under mutagenic effects of 3 main types: chemical, UV as a representative of non-penetrating radiation, and penetrating radiation permitted to characterize this compound as ''reparagen''. It was emphasized that the application of reparagens capable of intensifying or weakening the reparative process permitted to observe different effects of reparation dependence on the concentration of a chemical agent being introduced from outside and localize the process of reparagen effect in time

  13. Spectral variations of UV-A and PAR solar radiation in estuarine waters of Goa

    Digital Repository Service at National Institute of Oceanography (India)

    Talaulikar, M.; Suresh, T.; Silveira, N.; Desa, E.; Matondkar, S; Lotlikar, A

    radiation (400 to 700 nm), PAR and ultraviolet radiation in the range 350-400 nm (UV-A) are presented here. The mean PAR values at the surface were 327 W/m sup(2) and reduced to 84 W/m sup(2) at first optical depth, Z sub(90) (m) in water. The first optical...

  14. Choice of Eye-Safe Radiation Wavelength in UV and Near IR Spectral Bands for Remote Sensing

    OpenAIRE

    M. L. Belov; V. A. Gorodnichev; D. A. Kravtsov; A. A. Cherpakova

    2016-01-01

    The introduction of laser remote sensing systems carries a particular risk to the human’s sense of vision. A structure of the eye, and especially the retina, is the main critical organ as related to the laser radiation.The work uses the optical models of the atmosphere, correctly working in both the UV and the near-IR band, to select the eye-safe radiation wavelengths in the UV (0.355 m) and near-IR (~ 1.54 and ~ 2 m) spectral bands from the point of view of recorded lidar signal value to ful...

  15. Biobased Nanoparticles for Broadband UV Protection with Photostabilized UV Filters

    NARCIS (Netherlands)

    Hayden, D.R.|info:eu-repo/dai/nl/412640694; Imhof, A.|info:eu-repo/dai/nl/145641600; Velikov, K. P.|info:eu-repo/dai/nl/239483472

    2016-01-01

    Sunscreens rely on multiple compounds to provide effective and safe protection against UV radiation. UV filters in sunscreens, in particular, provide broadband UV protection but are heavily linked to adverse health effects due to the generation of carcinogenic skin-damaging reactive oxygen species

  16. Visible laser and UV-A radiation impact on a PNP degrading Moraxella strain and its rpoS mutant.

    Science.gov (United States)

    Nandakumar, Kanavillil; Keeler, Werden; Schraft, Heidi; Leung, Kam T

    2006-07-05

    The role of stationary phase sigma factor gene (rpoS) in the stress response of Moraxella strain when exposed to radiation was determined by comparing the stress responses of the wild-type (WT) and its rpoS knockout (KO) mutant. The rpoS was turned on by starving the WT cultures for 24 h in minimal salt medium. Under non-starved condition, both WT and KO planktonic Moraxella cells showed an increase in mortality with the increase in duration of irradiation. In the planktonic non-starved Moraxella, for the power intensity tested, UV radiation caused a substantially higher mortality rate than did by the visible laser light (the mortality rate observed for 15-min laser radiation was 53.4 +/- 10.5 and 48.7 +/- 8.9 for WT and KO, respectively, and 97.6 +/- 0 and 98.5 +/- 0 for 25 s of UV irradiation in WT and KO, respectively). However, the mortality rate decreased significantly in the starved WT when exposed to these two radiations. In comparison, rpoS protected the WT against the visible laser light more effectively than it did for the UV radiation. The WT and KO strains of Moraxella formed distinctly different types of biofilms on stainless steel coupons. The KO strain formed a denser biofilm than did the WT. Visible laser light removed biofilms from the surfaces more effectively than did the UV. This was true when comparing the mortality of bacteria in the biofilms as well. The inability of UV radiation to penetrate biofilms due to greater rates of surface absorption is considered to be the major reason for the weaker removal of biofilms in comparison to that of the visible laser light. This result suggests that high power visible laser light might be an effective tool for the removal of biofilms. (c) 2006 Wiley Periodicals, Inc.

  17. DNA repair synthesis in human skin exposed to ultraviolet radiation used in PUVA (psoralen and UV-A) therapy for psoriasis

    International Nuclear Information System (INIS)

    Bishop, S.C.

    1979-01-01

    The ultraviolet radiation used in psoralen and UV-A (PUVA) therapy stimulated DNA repair activity in normal human skin and in the uninvolved skin from psoriatic patients. The activity detected by autoradiography increased linearly with exposure time. No stimulation was observed when the UV-B component was removed from the incident radiation by filtration through glass. Therefore UV-B damage to DNA was found responsible for the activity detected following exposure to the unfiltered PUVA light source. (author)

  18. The effect of ultraviolet radiation on the pathogenesis of Candida albicans in mice

    International Nuclear Information System (INIS)

    Denkins, Y.M.

    1991-01-01

    This dissertation addresses questions concerning the effects of UV radiation on the pathogenesis of opportunistic fungal pathogens such as Candida albicans. UV radiation decreased the survival of Candida-infected mice; however, no correlation was found between suppression of the delayed type hypersensitivity (DTH) response and the course of lethal infection. This suggested that DTH was not protective against lethal disease with this organism. UV radiation also changed the persistence of the organism in the internal organs. UV-irradiated, infected animals had increased numbers of Candida in their kidneys compared to non-irradiated mice. Sensitization prior to UV irradiation aided clearance of the organism from the kidneys of UV-irradiated mice. These data show that UV radiation suppresses cell-mediated immunity to Candida albicans in mice and increases mortality of Candida-infected mice. Moreover, the data suggest that an increase in environmental UV radiation could increase the severity of pathogenic infections

  19. Instability of a double layer and initiation of a photoelectric gas discharge by UV radiation

    International Nuclear Information System (INIS)

    Meshalkin, E.A.

    1991-01-01

    At the present time there is no theory or systematic experiments for the conversion of the energy of vacuum uv radiation into current energy in connection with the photoelectric effect in gases. The aim in this work was to study the process by which an SCL is established and to obtain a gas discharge associated with photoemission (a photoelectric gas discharge), similar to the gas discharge in thermal-emission converters, in order to transform optical energy into electrical current. The development of a space-charge layer (SCL) is studied when a metallic target in air or nitrogen at a pressure in the range 10 -3 -10 torr is subjected to UV radiation. A laser plasma was used as the source of the UV radiation, modulated in intensity at a frequency of 75 MHz. It was found that after a period of development in the course of which the potential difference across the SCL can reach 185 V, it drops abruptly. This effect is due to an instability of the SCL which arises because the pressure of the electron gas in the background plasma exceeds the confining pressure of the electric field which penetrates from SCL into the plasma. A photoelectric arc discharge was observed in which the energy of the UV radiation was converted into current energy with an efficiency ∼ 1%. The current was found to be 3.1 A and the power in the load was 10W. Because of the threshold for the occurrence of the instability in the SCL, the potential drop across the SCL could be completely modulated at a frequency of 75 MHz. The amplitude of the HF current reached 0.3 A

  20. Dependence of biologically active UV radiation on the atmospheric ozone in 2000 - 2001 over Stara Zagora, Bulgaria

    International Nuclear Information System (INIS)

    Gogosheva, Tz.; Petkov, B.; Mendeva, B.; Krastev, D.

    2003-01-01

    This study investigates how the changes in simultaneously measured ozone columns influence the biologically active UV irradiance. Spectral ground-based measurements of direct solar ultraviolet radiation performed at Stara Zagora (42 o N, 25 o E), Bulgaria in 2000 - 2001 are used in conjunction with the total ozone content to investigate the relation to the biologically active UV radiation, depending on the solar zenith angle (SZA) and the ozone. The device measures the direct solar radiation in the range 290 - 360 nm at 1 nm resolution. The direct sun UV doses for some specific biological effects (erythema and eyes) are obtained as the integral in the wavelength interval between 290 and 330 nm of the UV solar spectrum weighted with an action spectrum, typical of each effect. For estimation of the sensitivity of biological doses to the atmospheric ozone we calculate the radiation amplification factor (RAF) defined as the percentage increase in the column amount of the atmospheric ozone. The biological doses increase significantly with the decrease of the SZA. The doses of SZA=20 o are about three times larger than doses at SZA=50 o . The RAF derived from our spectral measurements shows an increase of RAF along with the decreasing ozone. For example, the ozone reduction by 1% increases the erythemal dose by about 2%. (authors)

  1. Lethal effect of short-wave (254 nm) UV-radiation on cells of Chlamidomonas reinhardii strains with different carotenoid content

    International Nuclear Information System (INIS)

    Kamchatova, I.E.; Chunaev, A.S.; Bronnikov, V.A.

    1987-01-01

    In experiments on related Chlamidomonas reinhardii strains of similar mating type a study was made of sensitivity of cells with different carotenoid content to UV-radiation of 254 nm. Mutants having a lower, as opposed to the wild type strain, content of carotenoids exhibited an increased radiosensitivity. A carotenoid-free mutant was found to possess a higher sensitivity to UV-radiation which was typical of the strain with the impaired excision repair system. The studied subclone of the UV-radiosensitive strain CC-888 was unable to photoreactivate the UV-induced damages which was typical of the wild-type strain. The content of carotenoids in cells of this subnuclone exceeded that in cells of mutants with the reduced pigmentation

  2. Department of Radiation and Environmental Biology - Overview

    International Nuclear Information System (INIS)

    Cebulska-Wasilewska, A.

    2001-01-01

    Full text: In the year 2000 we completed our study of the genotoxic influence of occupational exposure to pesticides on human cells, and their susceptibility to radiation in particular. Examining blood samples from four countries: Greece, Hungary, Poland and Spain we found that exposure to pesticides usually resulted in an increased susceptibility to the UV-C radiation, although statistical significance could only be concluded for inhabitants of Poland. In Spain, exposure to pesticides was proved to impair the lymphocyte DNA repair capability, while for the Polish group this repair capability appeared enhanced in people exposed to pesticides (see the research reports below). The possible influence of lifestyle or particular diet on the observed national differences would probably be worth analyzing. We also investigate the biological effectiveness of therapeutic beams (neutrons and X-rays). Experimental part of such study, concerning neutrons of different mean energies, is over and the results are now being processed. Our work covers hot issues of environmental and radiation biology making us research partners to many domestic and foreign scientific institutions. Our proficiency in the field is also reflected by membership in various expert boards (e.g. evaluating research applications for the Fifth EU Framework Programme for RTD and Demonstration Activities in the field 'Environment and Health', lecturing in the 2000 NATO IOS Life Science Books). We have entered the 5 th EU Programme Scheme within the EXPAH project starting January 1, 2001. (author)

  3. Polymer-layered silicate nanocomposites by UV-radiation curing: an original synthesis

    International Nuclear Information System (INIS)

    Keller, L.; Decker, C.; Zahouily, K.; Miehe-Brendle, J.; Le Meins, J.M.

    2004-01-01

    Full text.Because of the many hopes which they raise, the nanocomposite materials are the subject of an increasing number of scientific publications. Indeed, the intimate association of a polymer matrix and silicate nano-platelets leads to the formation of materials having mechanical and barriers properties improved (fire, gas, humidity,...). A literature survey shows that these materials are generally produced by a thermal polymerization, which presents two major disadvantages: the use of organic solvents and a great consumption of energy. To overcome such limitations, photoinitiated polymerization was chosen to synthesize nanocomposite materials. By this technology, called UV radiation curing, a solvent-free resin is transformed within seconds into a solid polymer upon exposure to UV-radiation at ambient temperature. The principal objective of this study was to develop photopolymerizable systems with clay particles having a layer structure (phyllosilicates). The clay mineral was made organophilic by treatment with an alkylammonium salt to allow the acrylate resin to penetrate into the expanded galleries. A morphological characterization of the materials obtained was carried out by X-rays diffraction and electronic microscopy transmission. The polymerization of the various resins under the UV exposure was followed in situ by using the real-time infrared spectroscopy (RT-FTIR) and attenuated total reflection (ATR). The results obtained show that the presence of the organoclay does not modify much the polymerization kinetics. The nanocomposite material thus obtained is transparent, insoluble int eh organic solvents and presents improved mechanical properties, compared to the neat resin and the microcomposite, for a load factor ranging between 2 and 5% wt. The addition of nanoparticles also makes it possible to reduce efficiently the brightness of coatings UV and finally confers to this material barriers properties higher than that of the photocrosslinked polymeric

  4. Intercomparison of Environmental Nuclear Radiation Measuring

    Institute of Scientific and Technical Information of China (English)

    GAO; Fei; NI; Ning; HOU; Jin-bing; SONG; Ming-zhe

    2015-01-01

    In 2015,Radiation Metrology Division of China Institute of Atomic Energy organized an environmental monitoring of nuclear radiation measuring intercomparison,and 9laboratories attended.The intercomparison included environmental level dosemeters and protection level

  5. Phototransformation of membrane lipids and its role in biomembrane function change under the effect of UV-radiation

    International Nuclear Information System (INIS)

    Roshchupkin, D.I.; Anosov, A.K.; Murina, M.A.; Lordkipanidze, A.T.

    1988-01-01

    The papers devoted to the investigation of photochemical transformations of lipid under the effect of UV radiation of biological membranes are reviewed. The mechanism of peroxide photooxidation of mebrane lipid is considered. Data on the effect of antioxidants and the structure state of membranes on the process of peroxide photooxidation of lipid are presented. The problem on the role of this process under the effect of UV-radiation on blood and skin of mammals is discussed. 48 refs.; 4 refs

  6. Changes induced by UV radiation in the presence of sodium benzoate in films formulated with polyvinyl alcohol and carboxymethyl cellulose

    International Nuclear Information System (INIS)

    Villarruel, S.; Giannuzzi, L.; Rivero, S.; Pinotti, A.

    2015-01-01

    This work was focused on: i) developing single and blend films based on carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVOH) studying their properties, ii) analyzing the interactions between CMC and PVOH and their modifications UV-induced in the presence of sodium benzoate (SB), and iii) evaluating the antimicrobial capacity of blend films containing SB with and without UV treatment. Once the blend films with SB were exposed to UV radiation, they exhibited lower moisture content as well as a greater elongation at break and rougher surfaces compared to those without treatment. Considering oxygen barrier properties, the low values obtained would allow their application as packaging with selective oxygen permeability. Moreover, the characteristics of the amorphous phase of the matrix prevailed with a rearrangement of the structure of the polymer chain, causing a decrease of the crystallinity degree. These results were supported by X-rays and DSC analysis. FT-IR spectra reflected some degree of polymer–polymer interaction at a molecular level in the amorphous regions. The incorporation of sodium benzoate combined with UV treatment in blend films was positive from the microbial point of view because of the growth inhibition of a wide spectrum of microorganisms. From a physicochemical perspective, the UV treatment of films also changed their morphology rendering them more insoluble in water, turning the functionalized blend films into a potential material to be applied as food packaging. - Highlights: • CMC:PVOH blend films were developed with the addition of sodium benzoate (SB). • Exposition to UV radiation was carried out with sodium benzoate as photoinitiator. • Blend films were exposed to UV radiation to modify their surface morphology. • Low O 2 permeability of UV treated blends allow them to be used as selective packaging. • Efficacy of SB as an antimicrobial agent was examined with and without UV radiation

  7. Changes induced by UV radiation in the presence of sodium benzoate in films formulated with polyvinyl alcohol and carboxymethyl cellulose

    Energy Technology Data Exchange (ETDEWEB)

    Villarruel, S. [Faculty of Exact Sciences, UNLP (Argentina); Giannuzzi, L.; Rivero, S. [Center for Research and Development in Food Cryotechnology (CCT-CONICET La Plata), 47 and 116 (Argentina); Pinotti, A., E-mail: acaimpronta@hotmail.com [Center for Research and Development in Food Cryotechnology (CCT-CONICET La Plata), 47 and 116 (Argentina); Faculty of Engineering, UNLP, La Plata 1900 (Argentina)

    2015-11-01

    This work was focused on: i) developing single and blend films based on carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVOH) studying their properties, ii) analyzing the interactions between CMC and PVOH and their modifications UV-induced in the presence of sodium benzoate (SB), and iii) evaluating the antimicrobial capacity of blend films containing SB with and without UV treatment. Once the blend films with SB were exposed to UV radiation, they exhibited lower moisture content as well as a greater elongation at break and rougher surfaces compared to those without treatment. Considering oxygen barrier properties, the low values obtained would allow their application as packaging with selective oxygen permeability. Moreover, the characteristics of the amorphous phase of the matrix prevailed with a rearrangement of the structure of the polymer chain, causing a decrease of the crystallinity degree. These results were supported by X-rays and DSC analysis. FT-IR spectra reflected some degree of polymer–polymer interaction at a molecular level in the amorphous regions. The incorporation of sodium benzoate combined with UV treatment in blend films was positive from the microbial point of view because of the growth inhibition of a wide spectrum of microorganisms. From a physicochemical perspective, the UV treatment of films also changed their morphology rendering them more insoluble in water, turning the functionalized blend films into a potential material to be applied as food packaging. - Highlights: • CMC:PVOH blend films were developed with the addition of sodium benzoate (SB). • Exposition to UV radiation was carried out with sodium benzoate as photoinitiator. • Blend films were exposed to UV radiation to modify their surface morphology. • Low O{sub 2} permeability of UV treated blends allow them to be used as selective packaging. • Efficacy of SB as an antimicrobial agent was examined with and without UV radiation.

  8. Oxidative damage in response to natural levels of UV-B radiation in larvae of the tropical sea urchin Tripneustes gratilla.

    Science.gov (United States)

    Lister, Kathryn Naomi; Lamare, Miles D; Burritt, David J

    2010-01-01

    To assess the effects of UV radiation (280-400nm) on development, oxidative damage and antioxidant defence in larvae of the tropical sea urchin Tripneustes gratilla, a field experiment was conducted at two depths in Aitutaki, Cook Islands (18.85°S, 159.75°E) in May 2008. Compared with field controls (larvae shielded from UV-R but exposed to VIS-radiation), UV-B exposure resulted in developmental abnormality and increases in oxidative damage to proteins (but not lipids) in embryos of T. gratilla held at 1m depth. Results also indicated that larvae had the capacity to increase the activities of protective antioxidant enzymes when exposed to UV-B. The same trends in oxidative damage and antioxidant defence were observed for embryos held at 4m, although the differences were smaller and more variable. In contrast to UV-B exposure, larvae exposed to UV-A only showed no significant increases in abnormality or oxidative damage to lipids and proteins compared with field controls. This was true at both experimental depths. Furthermore, exposure to UV-A did not cause a significant increase in the activities of antioxidants. This study indicates that oxidative stress is an important response of tropical sea urchin larvae to exposure to UV radiation. © 2010 The Authors. Journal Compilation. The American Society of Photobiology.

  9. Comparison of UV action spectra for lethality and mutation in Salmonella typhimurium using a broad band source and monochromatic radiations

    International Nuclear Information System (INIS)

    Calkins, John; Selby, Christopher; Enoch, H.G.

    1987-01-01

    The UV-B region (280-320 nm) is thought to be primarily responsible for the mutagenic, lethal, and carcinogenic effects of solar radiation. We have conducted UV-B action spectroscopy for mutagenesis and survival of Ames' Salmonella typhimurium strain TA98 (uvrB, pKM101) using both monochromatic radiation from a dye laser and broader bandwidth radiation emitted from FS-20 sunlamps. A series of optical filters having different transmission cut-offs together with the sunlamp source provided bandwidths having successively less short wavelength components from which a ''broad band'' action spectrum was deduced. The two sets of action spectra differed both qualitatively and quantitatively: in comparison to the monochromatic action spectra, the ''broad band'' spectra showed up to a 200-fold reduced efficiency for both mutation induction and lethality by UV-B wavelengths. These results suggest a large protective effect of the background UV-A and/or visible radiations which were present during the broad spectrum irradiations and which are also present in solar radiation. Additional experiments show that to the extent tested this protective effect is not due to photo-reactivation or irradiance (dose rate) effects. (author)

  10. UV and X radiation effects on the stability of calcium halide phosphate phosphors. 1

    International Nuclear Information System (INIS)

    Tews, W.

    1983-01-01

    Intensity losses of several calcium halide phosphate phosphors have been investigated as a function of the time of irradiation with near UV and X radiation. The results show that antimony-containing foreign phases increase such losses. The directly excited manganese centre emission is much more lowered than the sensitized one. Detrimental effects of the 185 nm UV radiation are observable not only in the first minutes of irradiation but also over considerably extended periods. The sensitization effect caused by irradiation in different gases depends on the phosphor, especially on the content of antimony, and can be explained by the sorption of gaseous impurities at the phosphor surface so that the diffusion of photochemical reaction products from the surface is inhibited

  11. Limnoithona sinensis as refuge for bacteria: protection from UV radiation and chlorine disinfection in drinking water treatment.

    Science.gov (United States)

    Lin, Tao; Cai, Bo; Chen, Wei

    2014-11-01

    In this study, we tested the potential of Limnoithona sinensis to provide its attached bacteria refuge against disinfection. The experimental results indicated that in water devoid of zooplankton, both UV radiation and chlorine disinfection significantly decreased the viability of free-living bacteria. In the presence of L. sinensis, however, the attached bacteria could survive and rapidly recover from disinfection. This demonstrated that L. sinensis provided protection from external damage to various aquatic bacteria that were attached to its body. The surviving bacteria remained on L. sinensis after disinfection exposure, which enabled a rapid increase in the bacterial population followed by their subsequent release into the surrounding water. Compared with UV radiation, chlorine disinfection was more effective in terms of inactivating attached bacteria. Both UV radiation and chlorine disinfection had little effect in terms of preventing the spread of undesirable bacteria, due to the incomplete inactivation of the bacteria associated with L. sinensis.

  12. Measurements of environmental radioactivity and radiation in Taiwan

    International Nuclear Information System (INIS)

    Lin Yuming; Huang Chingchung

    1993-01-01

    Established in 1974, the Taiwan Radiation Monitoring Center (TRMC) is responsible for the environmental radiation surveillance and related fields in Taiwan. Its environmental radiation monitoring programs can be categorized into two parts: surveillance of natural ionizing radiation and surveillance of man-made ionizing radiation. For natural ionizing radiation, surveillance programs are mainly to establish the radiation baseline data including radon. For man-made ionizing radiation, surveillance programs include the radio-fallout surveillance and the environmental radiation monitoring around the nuclear facilities. This article summarizes the relevant studies carried out by TRMC in the recent years

  13. Transglutaminase involvement in UV-A damage to the eye lens

    International Nuclear Information System (INIS)

    Weinreb, Orly; Dovrat, A.

    1996-01-01

    Solar radiation is believed to be one of the major environmental factors involved in lens cataractogenesis. The purpose of the study was to investigate the mechanisms by which UV-A at 365 nm causes damage to the eye lens. Bovine lenses were placed in special culture cells for pre-incubation of 24 hr. The lenses were positioned so that the anterior surface faced the incident UV-A radiation source and were maintained in the cells during irradiation. After irradiation, lens optical quality was monitored throughout the culture period and lens epithelium, cortex and nuclear samples were taken for biochemical analysis. Transglutaminase activity in the lens was affected by the radiation. The activity of transglutaminase in lens epithelium cortex and nucleus increased as a result of the irradiation and then declined towards control levels during the culture period, as the lens recovered from the UV-A damage. Specific lens proteins αB and βB1 crystallins (the enzyme substrates) were analyzed by SDS polyacrylamid gel electrophoreses and immunoblotting with specific antibodies. Seventy-two hours after irradiation of 44.8 J cm -2 UV-A, αB crystallins were affected as was shown by the appearance of aggregation and degradation products. Some protein changes seem to be reversible. It appears that transglutaminase may be involved in the mechanism by which UV-A causes damage to the eye lens. (Author)

  14. Monitoring ultraviolet (UV) radiation inactivation of Cronobacter sakazakii in dry infant formula using Fourier transform infrared spectroscopy.

    Science.gov (United States)

    Liu, Qian; Lu, Xiaonan; Swanson, Barry G; Rasco, Barbara A; Kang, Dong-Hyun

    2012-01-01

    Cronobacter sakazakii is an opportunistic pathogen associated with dry infant formula presenting a high risk to low birth weight neonates. The inactivation of C. sakazakii in dry infant formula by ultraviolet (UV) radiation alone and combined with hot water treatment at temperatures of 55, 60, and 65 °C were applied in this study. UV radiation with doses in a range from 12.1 ± 0.30 kJ/m² to 72.8 ± 1.83 kJ/m² at room temperature demonstrated significant inactivation of C. sakazakii in dry infant formula (P radiation combining 60 °C hot water treatment increased inactivation of C. sakazakii cells significantly (P radiation on C. sakazakii inactivation kinetics (D value) were not observed in infant formula reconstituted in 55 and 65 °C water (P > 0.05). The inactivation mechanism was investigated using vibrational spectroscopy. Infrared spectroscopy detected significant stretching mode changes of macromolecules on the basis of spectral features, such as DNA, proteins, and lipids. Minor changes on cell membrane composition of C. sakazakii under UV radiation could be accurately and correctly monitored by infrared spectroscopy coupled with 2nd derivative transformation and principal component analysis. © 2011 Institute of Food Technologists®

  15. Performance Analysis of Si-Based Ultra-Shallow Junction Photodiodes for UV Radiation Detection

    NARCIS (Netherlands)

    Shi, L.

    2013-01-01

    This thesis presents a performance investigation of newly-developed ultra-shallow junction photodiodes (PureB-diodes) for ultraviolet (UV) radiation detection. The photodiodes are fabricated by pure boron chemical vapor deposition (PureB CVD) technology, which can provide nanometer-thin boron

  16. Physicochemical characterization, antioxidant activity and total phenolic content in 'Gala' apples subjected to different UV-C radiation doses

    Directory of Open Access Journals (Sweden)

    Thaís Gabrielle Dias

    2017-01-01

    Full Text Available UV-C radiation is a food preservation method aimed to extend the life of the product, inactivate microorganisms, and stimulate the synthesis of phenolic compounds. This study aimed to physicochemically characterize and evaluate the antioxidant activity and phenolic content of ‘Gala’ apples subjected to different UV-C radiation doses.The fruits were harvested, sanitized, selected and inserted into a UV-C radiation chamber, and different radiation doses were applied as follows:0 KJ m-2 (0 min., 0.68 KJ m-2 (2 minutes,2.73 KJ m-2 (4 minutes, and 4.10 KJ m-2 (6 minutes. The apples were stored for 120 days at 5 ± 1°C and analyzed after 0, 30, 60, 90, and 120 days of storage. Radiation doses had no influence on parameters, such as weight loss, firmness and Hue angle, and physicochemical aspects, such as pH, soluble solids, titratable acidity and the soluble solids/titratable acidity ratio. The 4.10 KJ m-2 dose was effective and increased the phenolic content and antioxidant activity for up to 90 days while maintaining the content of vitamin C during storage.

  17. UV and ionizing radiations induced DNA damage, differences and similarities

    Science.gov (United States)

    Ravanat, Jean-Luc; Douki, Thierry

    2016-11-01

    Both UV and ionizing radiations damage DNA. Two main mechanisms, so-called direct and indirect pathways, are involved in the degradation of DNA induced by ionizing radiations. The direct effect of radiation corresponds to direct ionization of DNA (one electron ejection) whereas indirect effects are produced by reactive oxygen species generated through water radiolysis, including the highly reactive hydroxyl radicals, which damage DNA. UV (and visible) light damages DNA by again two distinct mechanisms. UVC and to a lesser extend UVB photons are directly absorbed by DNA bases, generating their excited states that are at the origin of the formation of pyrimidine dimers. UVA (and visible) light by interaction with endogenous or exogenous photosensitizers induce the formation of DNA damage through photosensitization reactions. The excited photosensitizer is able to induce either a one-electron oxidation of DNA (type I) or to produce singlet oxygen (type II) that reacts with DNA. In addition, through an energy transfer from the excited photosensitizer to DNA bases (sometime called type III mechanism) formation of pyrimidine dimers could be produced. Interestingly it has been shown recently that pyrimidine dimers are also produced by direct absorption of UVA light by DNA, even if absorption of DNA bases at these wavelengths is very low. It should be stressed that some excited photosensitizers (such as psoralens) could add directly to DNA bases to generate adducts. The review will described the differences and similarities in terms of damage formation (structure and mechanisms) between these two physical genotoxic agents.

  18. Estimation of surface UV levels based on Meteor-3/TOMS ozone data

    Energy Technology Data Exchange (ETDEWEB)

    Borisov, Y A [Central Aerological Observatory, Moscow (Russian Federation); Geogdzhaev, I V [Moscow Inst. of Physics and Technology, Moscow (Russian Federation); Khattatov, V U [Central Aerological Observatory, Moscow (Russian Federation)

    1996-12-31

    The major consequence of ozone layer depletion for the environment is an increase of harmful ultraviolet (UV) radiation on the Earth surface and in the upper ocean. This implies the importance of environmental UV monitoring. Since the direct global monitoring is not currently possible, indirect estimations of surface UV levels may be used based on satellite ozone data (Madronich, S. 1992). Total Ozone Mapping Spectrometer (TOMS) on board the METEOR-3 satellite provided regular set of data for such estimates. During the time of its operation (August, 1991 - December, 1994) the instrument registered several ozone hole events over Antarctica, when ozone levels dropped by as much as 60 % from their unperturbed values. Probably even more alarming ozone depletions were observed over highly populated regions of middle latitudes of northern hemisphere. Radiative transfer modeling was used to convert METEOR-3/TOMS daily ozone values into regional and global maps of biologically active UV. Calculations demonstrate the effect on surface UV levels produced by ozone hole over Antarctica and ozone depletions over the territory of Russia (March, 1994). UV contour lines deviate from the normal appearance which is determined by growing southward solar elevation. UV contour lines are almost perpendicular to the ozone ones in the ozone depletions areas. The 30 % ozone depletion, over Siberia caused more than 30 % increase in noontime erythemal UV levels, which is equivalent to 10-15 degrees southward latitude displacement. Higher UV radiation increases were found in ozone hole over South America (October 1992) equivalent to about 20 degrees southward displacement

  19. Estimation of surface UV levels based on Meteor-3/TOMS ozone data

    Energy Technology Data Exchange (ETDEWEB)

    Borisov, Y.A. [Central Aerological Observatory, Moscow (Russian Federation); Geogdzhaev, I.V. [Moscow Inst. of Physics and Technology, Moscow (Russian Federation); Khattatov, V.U. [Central Aerological Observatory, Moscow (Russian Federation)

    1995-12-31

    The major consequence of ozone layer depletion for the environment is an increase of harmful ultraviolet (UV) radiation on the Earth surface and in the upper ocean. This implies the importance of environmental UV monitoring. Since the direct global monitoring is not currently possible, indirect estimations of surface UV levels may be used based on satellite ozone data (Madronich, S. 1992). Total Ozone Mapping Spectrometer (TOMS) on board the METEOR-3 satellite provided regular set of data for such estimates. During the time of its operation (August, 1991 - December, 1994) the instrument registered several ozone hole events over Antarctica, when ozone levels dropped by as much as 60 % from their unperturbed values. Probably even more alarming ozone depletions were observed over highly populated regions of middle latitudes of northern hemisphere. Radiative transfer modeling was used to convert METEOR-3/TOMS daily ozone values into regional and global maps of biologically active UV. Calculations demonstrate the effect on surface UV levels produced by ozone hole over Antarctica and ozone depletions over the territory of Russia (March, 1994). UV contour lines deviate from the normal appearance which is determined by growing southward solar elevation. UV contour lines are almost perpendicular to the ozone ones in the ozone depletions areas. The 30 % ozone depletion, over Siberia caused more than 30 % increase in noontime erythemal UV levels, which is equivalent to 10-15 degrees southward latitude displacement. Higher UV radiation increases were found in ozone hole over South America (October 1992) equivalent to about 20 degrees southward displacement

  20. High Power UV LED Industrial Curing Systems

    Energy Technology Data Exchange (ETDEWEB)

    Karlicek, Robert, F., Jr; Sargent, Robert

    2012-05-14

    UV curing is a green technology that is largely underutilized because UV radiation sources like Hg Lamps are unreliable and difficult to use. High Power UV LEDs are now efficient enough to replace Hg Lamps, and offer significantly improved performance relative to Hg Lamps. In this study, a modular, scalable high power UV LED curing system was designed and tested, performing well in industrial coating evaluations. In order to achieve mechanical form factors similar to commercial Hg Lamp systems, a new patent pending design was employed enabling high irradiance at long working distances. While high power UV LEDs are currently only available at longer UVA wavelengths, rapid progress on UVC LEDs and the development of new formulations designed specifically for use with UV LED sources will converge to drive more rapid adoption of UV curing technology. An assessment of the environmental impact of replacing Hg Lamp systems with UV LED systems was performed. Since UV curing is used in only a small portion of the industrial printing, painting and coating markets, the ease of use of UV LED systems should increase the use of UV curing technology. Even a small penetration of the significant number of industrial applications still using oven curing and drying will lead to significant reductions in energy consumption and reductions in the emission of green house gases and solvent emissions.

  1. Investigation of 207 nm UV radiation for degradation of organic dye ...

    African Journals Online (AJOL)

    The photo-degradation of organic dye C.I. Acid Red 213 (AR-213) was achieved by 207 nm UV radiation emitted from a planar KrBr* excimer lamp without addition of oxidants at varying initial pH values. Precipitates were found to be generated when the irradiated solution of initial acid pH was adjusted to alkaline pH and ...

  2. Environmental radiation monitoring from the decommission of TRIGA

    Energy Technology Data Exchange (ETDEWEB)

    Choi Geun Sik; Lee, Chang Woo [Korea Atomic Energy Research Institute, Taejon (Korea)

    1999-02-01

    Environmental Radiation Monitoring was carried out with measurement of environment radiation and environment radioactivity analysis around TRIGA Research Reactor. The results of environmental radiation monitoring around TRIGA Research Reactor are the follows: The average level of environmental radiation dose measured by potable ERM and accumulated radiation dose by TLD was almost same level compared with the past years. Gross {beta} radioactivity in environmental samples showed a environmental level. v-radionuclides in water samples were not detected. But only radionuclide K-40, which is natural radionuclide, was detected in the all samples and Cs-137 was detected in the surface soil and discharge sediment. 37 refs., 12 figs., 31 tabs. (Author)

  3. The optimal UV exposure time for vitamin D3 synthesis and erythema estimated by UV observations in Korea

    Science.gov (United States)

    Lee, Y. G.; Koo, J. H.

    2016-12-01

    Solar UV radiation in a wavelength range between 280 to 400 nm has both positive and negative influences on human body. Surface UV radiation is the main natural source of vitamin D, providing the promotion of bone and musculoskeletal health and reducing the risk of a number of cancers and other medical conditions. However, overexposure to surface UV radiation is significantly related with the majority of skin cancer, in addition other negative health effects such as sunburn, skin aging, and some forms of eye cataracts. Therefore, it is important to estimate the optimal UV exposure time, representing a balance between reducing negative health effects and maximizing sufficient vitamin D production. Previous studies calculated erythemal UV and vitamin-D UV from the measured and modelled spectral irradiances, respectively, by weighting CIE Erythema and Vitamin D3 generation functions (Kazantzidis et al., 2009; Fioletov et al., 2010). In particular, McKenzie et al. (2009) suggested the algorithm to estimate vitamin-D production UV from erythemal UV (or UV index) and determined the optimum conditions of UV exposure based on skin type Ⅱ according to the Fitzpatrick (1988). Recently, there are various demands for risks and benefits of surface UV radiation on public health over Korea, thus it is necessary to estimate optimal UV exposure time suitable to skin type of East Asians. This study examined the relationship between erythemally weighted UV (UVEry) and vitamin D weighted UV (UVVitD) from spectral UV measurements during 2006-2010. The temporal variations of the ratio (UVVitD/UVEry) were also analyzed and the ratio as a function of UV index was applied to the broadband UV measured by UV-Biometer at 6 sites in Korea Thus, the optimal UV exposure time for vitamin D3 synthesis and erythema was estimated for diurnal, seasonal, and annual scales over Korea. In summer with high surface UV radiation, short exposure time leaded to sufficient vitamin D and erythema and vice

  4. Leaf waxes of slow-growing alpine and fast-growing lowland Poa species: inherent differences and responses to UV-B radiation

    International Nuclear Information System (INIS)

    Pilon, J.J.; Lambers, H.; Baas, W.; Tosserams, M.; Rozema, J.; Atkin, O.K.

    1999-01-01

    We investigated whether alpine and lowland Poa species exhibit inherent differences in leaf cuticular waxes, leaf UV absorbing compounds and/or growth responses to UV-B treatment. All plants were grown hydroponically in a growth cabinet (constant 20°; 14 hr photoperiod; 520 μmol photons m −2 s −1 PAR). Two alpine (P. fawcettiae and P. costiniana), one sub-alpine (P. alpina) and three temperate lowland species (P. pratensis, P. compressa and P. trivialis) were grown under conditions without UV radiation for 36 days. In a subsequent experiment, four Poa species (P. costiniana, P. alpina, P. compressa and P. trivialis) were also exposed for 21 days to UV-B/(UV-A) radiation ('UV-B treatment') that resulted in daily UV-B radiation of 7.5 kJ m −2 day −1 , with control plants being grown without UV-B ('UV-A control treatment'). All treatments were carried out in the same growth cabinet. There was no altitudinal trend regarding wax concentrations per unit leaf area, when the six species grown under UV-less conditions, were compared at similar developmental stage (20–30 g shoot fresh mass). However, large differences in cuticular wax chemical composition were observed between the alpine and lowland species grown under UV-less conditions. For example, a single primary alcohol was present in the waxes of the lowland and sub-alpine species (C 26 H 53 OH), but was virtually absent in the alpine species. Although alkanes were present in all six species (primarily C 29 H 60 and C 31 H 64 ), the proportion of total wax present as alkanes was highest in the alpine species. Aldehydes were only present in the waxes of the alpine species. Conversely, substantial amounts of triterpenoids were mainly present in the three lowland species (squalene and lupeol were the dominant forms). The proportion of total wax present as long-chain esters (LCE-s) was similar in all six species grown in the absence of UV radiation. Acetates were observed only in the wax of

  5. Environmental γ radiation monitor

    International Nuclear Information System (INIS)

    Qu Xiaopeng

    1993-01-01

    The environmental γ radiation monitor is a kind of dose or dose rate measuring devices, which can be used for monitoring environmental γ radiation around a nuclear site when normal or even abnormal events occur. The monitor is controlled by a single-chip microcomputer so that it can acquire synchronously the data from four detectors and transfer the data to a central computer. The monitor has good temperature property due to the technique of temperature correction. The monitor has been used in the environment monitoring vehicle for Qinshan Nuclear Power Plant

  6. Solar UV-B radiation and ethylene play a key role in modulating effective defenses against Anticarsia gemmatalis larvae in field-grown soybean.

    Science.gov (United States)

    Dillon, Francisco M; Tejedor, M Daniela; Ilina, Natalia; Chludil, Hugo D; Mithöfer, Axel; Pagano, Eduardo A; Zavala, Jorge A

    2018-02-01

    Solar UV-B radiation has been reported to enhance plant defenses against herbivore insects in many species. However, the mechanism and traits involved in the UV-B mediated increment of plant resistance are unknown in crops species, such as soybean. Here, we studied defense-related responses in undamaged and Anticarsia gemmatalis larvae-damaged leaves of two soybean cultivars grown under attenuated or full solar UV-B radiation. We determined changes in jasmonates, ethylene (ET), salicylic acid, trypsin protease inhibitor activity, flavonoids, and mRNA expression of genes related with defenses. ET emission induced by Anticarsia gemmatalis damage was synergistically increased in plants grown under solar UV-B radiation and was positively correlated with malonyl genistin concentration, trypsin proteinase inhibitor activity and expression of IFS2, and the pathogenesis protein PR2, while was negatively correlated with leaf consumption. The precursor of ET, aminocyclopropane-carboxylic acid, applied exogenously to soybean was sufficient to strongly induce leaf isoflavonoids. Our results showed that in field-grown soybean isoflavonoids were regulated by both herbivory and solar UV-B inducible ET, whereas flavonols were regulated by solar UV-B radiation only and not by herbivory or ET. Our study suggests that, although ET can modulate UV-B-mediated priming of inducible plant defenses, some plant defenses, such as isoflavonoids, are regulated by ET alone. © 2017 John Wiley & Sons Ltd.

  7. The Effect Of Enhanced UV-B Radiation On Norway Spruce (Picea Abies (L.) Karst.) And Consequences For The Mountain Forest; Ucinek Ultravijolicnega Sevanja Na Smreko (Picea abies (L.) Karst.) In Posledice Za Garski Gozdni Ekosistem

    Energy Technology Data Exchange (ETDEWEB)

    Trošt Sedej, T.

    2005-07-01

    NaNorway spruce trees from the sub alpine stand are exposed not only to high UV-B radiation but also to a complex of other environmental factors, such as high photosynthetically active radiation, extreme temperature conditions, deficient water and mineral supply, which might cause stress responses. Current year needles from the sub alpine stand exhibited lower photochemical efficiency and total chlorophyll content compared to samples from lower altitudes. The result suggested that young needles were most vulnerable to stress factors, since the protective mechanisms were not fully developed. Current+1 year needles from the sub alpine stand exhibited lower ETSvalues and higher total UV-B absorbing compounds, which may be interpreted as most successful protection against UVB radiation of current+1 year needles among the three needle age classes.Despite the obtained results, the effect of a single stress factor on spruce could not be easily drawn out. Still, we may assume that the spruce is quite tolerant to high UV-B radiation and other extreme environmental factors in the mountains. [Serbian] Rastline, ki uspevajo v gorah, so pogosto izpostavljene stresnim razmeram, predvsem pove ani jakosti sevanja UV-B, skrajnim temperaturnim razmeram ter pomanjkanju vode in hranil. Odziv smreke na okoljske razmere je kompleksen. Pri enoletnih iglicah v visokogorju smo izmerili manjšo fotokemi no u inkovitost in vsebnost klorofilov, kar kaže na ob utljivost mladih iglic, kjer zaš itni mehanizmi še niso dokon no razviti. Pri starejših iglicah razlika ni bila ve statisti no zna ilna, zato sklepamo, da se poškodbe v drugem in tretjem letu prepre ijo ali popravijo. Pri dveletnih iglicah smreke z visokogorskega rastiš a je bil dihalni potencial zna ilno manjši in vsebnost UV-B absorbirajo ih snovi zna ilno ve ja, kar pojasnjujemo s tem, da so dveletne iglice z visokogorskega rastiš a med tremi starostnimi razredi najbolj odporne proti UV-B sevanju. Iz rezultatov sicer ne moremo

  8. Influence of UV and Gamma radiations on the induced birefringence of stretched poly(vinyl) alcohol foils

    Science.gov (United States)

    Nechifor, Cristina-Delia; Zelinschi, Carmen Beatrice; Dorohoi, Dana-Ortansa

    2014-03-01

    The aim of our paper is to evidence the influence of Gamma and UV radiations on the induced birefringence of poly(vinyl alcohol) stretched foils. Thin foils of PVA were prepared and dried without modifying their surfaces. The polymeric foils were irradiated from 15 min to 6 h using UV and Gamma radiations. The induced by stretching under heating birefringence of PVA films was measured at λ = 589.3 nm with a Babinet Compensator. Physico-chemical processes (photo stabilization, photo degradation, oxidation) induced by irradiation of polymer matrix influence both the stretching degree and the anisotropy of etired foils. An increase of birefringence versus the stretching ratio of the PVA foils was evidenced for all studied samples. The dependence of the birefringence on the exposure time, stretching ratio and nature of radiation was also confirmed.

  9. Spatial variability of ultraviolet-absorbing compounds in an aquatic liverwort and their usefulness as biomarkers of current and past UV radiation: A case study in the Atlantic–Mediterranean transition

    International Nuclear Information System (INIS)

    Monforte, Laura; Tomás-Las-Heras, Rafael; Del-Castillo-Alonso, María-Ángeles; Martínez-Abaigar, Javier; Núñez-Olivera, Encarnación

    2015-01-01

    The spatial variability of ultraviolet-absorbing compounds (UVACs) in the freshwater liverwort Jungermannia exsertifolia subsp. cordifolia was studied in mid-latitudes (the Atlantic–Mediterranean transition) across a wide lati-altitudinal gradient, with the aim of testing the usefulness of UVACs as biomarkers of current ambient levels of UV radiation. We analysed 17 samples from streams located in the main mountain ranges of the Iberian Peninsula, differentiating methanol-soluble (SUVACs, mainly located in the vacuoles) and methanol-insoluble (IUVACs, bound to cell walls) compounds, since they represent different manners to cope with UV radiation. In both fractions, the bulk level of UVACs and the concentrations of several individual compounds were measured. In addition, we measured F v /F m , DNA damage and sclerophylly index (SI) as possible additional UV biomarkers. UVACs showed a high variability, probably due not only to the gradients of macroenvironmental factors (UV radiation, PAR, and water temperature), but also to microenvironmental factors inherent to the dynamic nature of mountain streams. Two soluble coumarins were positively correlated with UV levels and could be used for ambient UV biomonitoring in the spatial scale. In contrast to the variability in UVACs, the relatively homogeneous values of F v /F m and the lack of any DNA damage made these variables useless for ambient UV biomonitoring, but suggested a strong acclimation capacity of this liverwort to changing environmental conditions (in particular, to UV levels). Finally, UVACs of fresh samples of the liverwort were compared to those of herbarium samples collected in the same lati-altitudinal gradient. SUVACs were significantly higher in fresh samples, whereas IUVACs generally showed the contrary. Thus, IUVACs were more stable than SUVACs and hence more adequate for retrospective UV biomonitoring. In conclusion, UVAC compartmentation should be taken into account for bryophyte-based UV

  10. Spatial variability of ultraviolet-absorbing compounds in an aquatic liverwort and their usefulness as biomarkers of current and past UV radiation: A case study in the Atlantic–Mediterranean transition

    Energy Technology Data Exchange (ETDEWEB)

    Monforte, Laura; Tomás-Las-Heras, Rafael; Del-Castillo-Alonso, María-Ángeles; Martínez-Abaigar, Javier, E-mail: javier.martinez@unirioja.es; Núñez-Olivera, Encarnación

    2015-06-15

    The spatial variability of ultraviolet-absorbing compounds (UVACs) in the freshwater liverwort Jungermannia exsertifolia subsp. cordifolia was studied in mid-latitudes (the Atlantic–Mediterranean transition) across a wide lati-altitudinal gradient, with the aim of testing the usefulness of UVACs as biomarkers of current ambient levels of UV radiation. We analysed 17 samples from streams located in the main mountain ranges of the Iberian Peninsula, differentiating methanol-soluble (SUVACs, mainly located in the vacuoles) and methanol-insoluble (IUVACs, bound to cell walls) compounds, since they represent different manners to cope with UV radiation. In both fractions, the bulk level of UVACs and the concentrations of several individual compounds were measured. In addition, we measured F{sub v}/F{sub m}, DNA damage and sclerophylly index (SI) as possible additional UV biomarkers. UVACs showed a high variability, probably due not only to the gradients of macroenvironmental factors (UV radiation, PAR, and water temperature), but also to microenvironmental factors inherent to the dynamic nature of mountain streams. Two soluble coumarins were positively correlated with UV levels and could be used for ambient UV biomonitoring in the spatial scale. In contrast to the variability in UVACs, the relatively homogeneous values of F{sub v}/F{sub m} and the lack of any DNA damage made these variables useless for ambient UV biomonitoring, but suggested a strong acclimation capacity of this liverwort to changing environmental conditions (in particular, to UV levels). Finally, UVACs of fresh samples of the liverwort were compared to those of herbarium samples collected in the same lati-altitudinal gradient. SUVACs were significantly higher in fresh samples, whereas IUVACs generally showed the contrary. Thus, IUVACs were more stable than SUVACs and hence more adequate for retrospective UV biomonitoring. In conclusion, UVAC compartmentation should be taken into account for bryophyte

  11. Study of mechanical behavior of PMMA in bending and after UV irradiation and gamma radiation

    International Nuclear Information System (INIS)

    Todt, M.L.; Kienen, V.D.; Azevedo, E.C.

    2014-01-01

    PMMA is a polymer that has density similar to water and refractive index alike to glass. It has been used in the substitution to roofing tiles and coverages, affording to be exposed to UV radiation and gamma radiation. This paper had the objective to study the effect in the flexural proprieties of the PMMA exposed to these types of radiations and the evaluation of the wettability through a contact angle measurer. The PMMA specimens have been submitted to 1500 h of UVA radiation, 1500 h of UVC radiation and to 25kGy of gamma radiation. The results show that the PMMA. (author)

  12. Effects of enhanced UV-B radiation on nitrogen fixation in arctic ecosystems.

    NARCIS (Netherlands)

    Solheim, B.; Zielke, M.; Bjerke, J.W.; Rozema, J.

    2006-01-01

    Recent global climate models predict a further significant loss of ozone in the next decades, with up to 50% depletion of the ozone layer over large parts of the Arctic resulting in an increase in ultraviolet-B radiation (UV-B) (280-315 nm) reaching the surface of the Earth. The percentage of total

  13. UV-A enhanced growth and UV-B induced positive effects in the recovery of photochemical yield in Gracilaria lemaneiformis (Rhodophyta).

    Science.gov (United States)

    Xu, Juntian; Gao, Kunshan

    2010-09-02

    The effects of solar UV radiation (280-400 nm) on growth, quantum yield and pigmentation in Gracilaria lemaneiformis were investigated when the thalli were cultured under solar radiation with or without UV for a period of 15 days. Presence of UV-A (315-400 nm) enhanced the relative growth rate, while UV-B (218-315 nm) inhibited it. The positive effect of UV-A and negative effect of UV-B counteracted to result in an insignificant impact of UVR on growth. During the noon period, both UV-A and UV-B resulted in the decrease of maximum quantum yield (Fv/Fm), but UV-B aided in the recovery of the yield in the late afternoon, reflecting that UV-B might be used as a signal in photorepair processes. UV induced the accumulation of UV-absorbing compounds (UVAC) to defend against the harmful UVR. However, the accumulation of UVAC took a much longer time compared to that previously reported, which was probably due to the lower levels of solar radiation and water temperature in the early spring period. Unknown UV-absorbing compounds (UVAC), which peaked at 265 nm, probably the precursor of MAAs (UVAC(325)), accumulated under moderate levels of solar radiation and were transformed to MAAs under higher solar radiation. Copyright (c) 2010 Elsevier B.V. All rights reserved.

  14. Comparison of Five Modeling Approaches to Quantify and Estimate the Effect of Clouds on the Radiation Amplification Factor (RAF) for Solar Ultraviolet Radiation

    Data.gov (United States)

    U.S. Environmental Protection Agency — Ultraviolet Radiation (UV) data collected at 21 US Environmental Protection Agency sites throughout the continental US, Alaska, Hawaii, and the US Virgin Islands...

  15. ROS and calcium signaling mediated pathways involved in stress responses of the marine microalgae Dunaliella salina to enhanced UV-B radiation.

    Science.gov (United States)

    Zhang, Xinxin; Tang, Xuexi; Wang, Ming; Zhang, Wei; Zhou, Bin; Wang, You

    2017-08-01

    UV-B ray has been addressed to trigger common metabolic responses on marine microalgae, however, the upstream events responsible for these changes in marine microalgae are poorly understood. In the present study, a species of marine green microalgae Dunaliella salina was exposed to a series of enhanced UV-B radiation ranging from 0.25 to 1.00 KJ·m -2 per day. The role of ROS and calcium signaling in the D. salina responses to UV-B was discussed. Results showed that enhanced UV-B radiation markedly decreased the cell density in a dose-dependent manner, but the contents of protein and glycerol that were essential for cell growth increased. It suggested that it was cell division instead of cell growth that UV-B exerted negative effects on. The subcellular damages on nuclei and plasmalemma further evidenced the hypothesis. The nutrient absorption was affected with UV-B exposure, and the inhibition on PO 4 3- uptake was more serious compared to NO 3 - uptake. UV-B radiation promoted reactive oxygen species (ROS) formation and thiobarbituric acid reactive substances (TBARS) contents, decreased the redox status and altered the antioxidant enzyme activities. The addition of the ROS scavenger and the glutathione biosynthesis precursor N-acetyl-l-cysteine (NAC) alleviated the stress degree, implying ROS-mediated pathway was involved in the stress response to UV-B radiation. Transient increase in Ca 2+ -ATPase was triggered simultaneously with UV-B exposure. Meanwhile, the addition of an intracellular free calcium chelator aggravated the damage of cell division, but exogenous calcium and ion channel blocker applications did not, inferring that endogenously initiated calcium signaling played roles in response to UV-B. Cross-talk analysis showed a relatively clear relationship between ROS inhibition and Ca 2+ -ATPase suppression, and a relation between Ca 2+ inhibition and GPx activity change was also observed. It was thus presumed that ROS-coupled calcium signaling via the

  16. Is ultraviolet radiation a synergistic stressor in combined exposures? The case study of Daphnia magna exposure to UV and carbendazim

    International Nuclear Information System (INIS)

    Ribeiro, Fabianne; Ferreira, Nuno C.G.; Ferreira, Abel; Soares, Amadeu M.V.M.; Loureiro, Susana

    2011-01-01

    The toxicological assessment of chemical compounds released to the environment is more accurate when mixtures of chemicals and/or interactions between chemicals and natural stressors are considered. Ultraviolet radiation can be taken as a natural stressor since the levels of UV are increasing due to the decrease of its natural filter, the stratospheric ozone concentration. Therefore, a combination of chemical exposures and increasing UV irradiance in aquatic environments is likely to occur. In the current study, combined effects of carbendazim and ultraviolet radiation were evaluated, using selected life traits as endpoints on Daphnia magna. To design combined exposures, first single chemical and natural stressor bioassays were performed: a reproduction test with carbendazim and a reproduction, feeding inhibition and Energy budget test with ultraviolet radiation. Following single exposures, the combinations of stressors included exposures to UV radiation and carbendazim for a maximum exposure time of 4 h, followed by a post-exposure period in chemically contaminated medium for a maximum of 15 days, depending on the endpoint, where the effects of the combined exposures were investigated. Statistical analyses of the data set were performed using the MixTox tool and were based on the conceptual model of Independent Action (IA) and possible deviations to synergism or antagonism, dose-ratio or dose-level response pattern. Both ultraviolet radiation and carbendazim as single stressors had negative impacts on the measured life traits of daphnids, a decrease on both feeding rates and reproduction was observed. Feeding rates and reproduction of D. magna submitted to combined exposures of ultraviolet radiation and carbendazim showed a dose-ratio deviation from the conceptual model as the best description of the data set, for both endpoints. For feeding inhibition, antagonism was observed when the UV radiation was the dominant item in combination, and for reproduction

  17. Environmental radiation monitoring around the nuclear facilities

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Chang Woo

    2012-03-15

    Environmental Radiation Monitoring was carried out with measurement of environment. radiation and environmental radioactivity analysis on the sites of KAERI nuclear facilities and Seoul Research Reactors and their environments. The average level of environmental radiation dose measured by an ERM and the accumulated radiation dose by a TLD were almost same level compared with the previous years. The activity of gross {alpha} and gross {beta}, Tritium, Uranium and Strontium in environmental samples showed a environmental level. The {gamma}-radionuclides such as natural radionuclides 40K or 7Be were detected in pine needle and food. The nuclear radionuclides 134Cs, 137Cs or 131I were temporarily detected in the samples of air particulate and rain in April and of fall out in 2nd quarter from the effect of Fukusima accident.

  18. Study of the anticorrelations between ozone and UV-B radiation using linear and exponential fits in Southern Brazil

    Science.gov (United States)

    Guarnieri, R.; Padilha, L.; Guarnieri, F.; Echer, E.; Makita, K.; Pinheiro, D.; Schuch, A.; Boeira, L.; Schuch, N.

    Ultraviolet radiation type B (UV-B 280-315nm) is well known by its damage to life on Earth, including the possibility of causing skin cancer in humans. However, the atmo- spheric ozone has absorption bands in this spectral radiation, reducing its incidence on Earth's surface. Therefore, the ozone amount is one of the parameters, besides clouds, aerosols, solar zenith angles, altitude, albedo, that determine the UV-B radia- tion intensity reaching the Earth's surface. The total ozone column, in Dobson Units, determined by TOMS spectrometer on board of a NASA satellite, and UV-B radiation measurements obtained by a UV-B radiometer model MS-210W (Eko Instruments) were correlated. The measurements were obtained at the Observatório Espacial do Sul - Instituto Nacional de Pesquisas Espaciais (OES/CRSPE/INPE-MCT) coordinates: Lat. 29.44oS, Long. 53.82oW. The correlations were made using UV-B measurements in fixed solar zenith angles and only days with clear sky were selected in a period from July 1999 to December 2001. Moreover, the mathematic behavior of correlation in dif- ferent angles was observed, and correlation coefficients were determined by linear and first order exponential fits. In both fits, high correlation coefficients values were ob- tained, and the difference between linear and exponential fit can be considered small.

  19. Algorithms and programs for processing of satellite data on ozone layer and UV radiation levels

    International Nuclear Information System (INIS)

    Borkovskij, N.B.; Ivanyukovich, V.A.

    2012-01-01

    Some algorithms and programs for automatic retrieving and processing ozone layer satellite data are discussed. These techniques are used for reliable short-term UV-radiation levels forecasting. (authors)

  20. Inhibition of Photo-Genotoxic Effects of UV Radiation on Human Peripheral Blood Lymphocites by Echinacea Purpurea (L.) Moench Herbal Extract

    International Nuclear Information System (INIS)

    Segvic Klaric, M.; Kosalec, I.; Vladimir-Knezevic, S.; Blazekovic, B.; Milic, M.; Kopjar, N.

    2011-01-01

    Ultraviolet (UV) radiation has many negative effects on human skin, including acute and chronic inflammation and oxidative stress which might cause DNA damage leading to skin photoaging and photocarcinogenesis. It was suggested that intake of phenolic acids, which are active components of some medicinal plants, might reduce DNA damage caused by UV radiation. Therefore, the purpose of this study was to check wheather the pretreatment of human peripheral blood lymphocytes with lyophilisate of Echinacea purpurea (L.) Moench (EH) extract (1 and 10 mg/mL) could reduce or prevent primary DNA damage induced by UVC radiation (253.7 nm) in laboratory conditions. Primary DNA damage was studied using the alkaline comet assay on isolated human blood lymphocytes. Plant extract used in this experiment contains phenolic acids (3.47 %), flavonoids (0.13 %), tannins (0.86 %) and proanthocyanidins (0.26 %). HPLC analysis showed that lyophilisate of EH extract contains 3.65 % of chicoric acid. Exposure of lymphocytes to UV radiation (30 and 60 min) caused a significant increase in the level of primary DNA damage (P < 0.001). Pretreatment of cells with both concentrations of EH was not genotoxic, and successfully protected the cells against the effects of UV radiation (30 min). Both concentrations of EH significantly reduced comet tail length after 60 min of UV radiation, while only pre-treatment with 1 mg/mL significantly reduced the values of tail intensity and tail moment (P < 0.001). Positive results obtained in this study speak in favour of continuing the research on effectiveness of Echinacea purpurea preparations and their potential application in developing cosmetic products for skin protection. (author)

  1. Identification of Native Bacteria of the Candelaria and Tatacoa Semiarid Zone, Capable of Withstanding a Mars UV Radiation Simulation

    Science.gov (United States)

    Mendez, Y.; Vives, M.

    2017-07-01

    This work is the first study to describe native bacteria from the semi-arid areas in Candelaria and Tatacoa in Colombia, able to withstand a simulation of UV radiation, in order to draw an analogy with microbial growth on the surface of Mars. Sampling was carried out in the areas mentioned taking 50 samples of sediment divided into 25 samples of surface and 25 deep samples. As soon as the samples were transferred, they were subjected to a test of UV radiation in an atmospheric simulation chamber designed for the experiment, for periods of 1, 6 and 12 hours of exposure. Microbiological analysis as a method of plate dilution and isolation were performed using the modified AIS growth medium, macroscopic and microscopic description of morphotypes, biochemical identification of the morphotypes found, extraction of the feasible mycelium, DNA extraction and amplification of the gene 16 S by PCR. 13 morphotypes of bacteria resistant to UV radiation were found, mostly compatible with the gender of Streptomyces. One of the morphotypes found resisted 12 hours exposure. Molecular analyzes did not produce any results, because it was not possible to amplify the 16S by PCR, this may be due to that the exposure to UV radiation can degrade the DNA in existence, a affecting the results. The finding of native bacteria capable of withstanding conditions UV radiation can give us an approximation of microbial growth, mechanisms of resistance and survival under extreme conditions such as those found on Mars, in order to develop biotechnological applications and establish planetary analogues to understand the origin and evolution of the universe.

  2. Responses of phylloplane yeasts to UV-B (290-320 nm) radiation: interspecific differences in sensitivity

    International Nuclear Information System (INIS)

    Gunasekera, T.S.; Paul, N.D.; Ayres, P.G.

    1997-01-01

    The sensitivity to UV-B (290–320 nm) radiation of common phylloplane yeasts from two contrasting UV-B environments was compared in the laboratory using mixtures of white light (PAR: 400–700 nm) and UV-B radiation from artificial lamp sources. Sporidiobolus salmonicolor, Rhodotorula mucilaginosa and Cryptococcus sp., the dominant yeasts on leaves of tea (Camellia sinensis), were isolated in Sri Lanka (SL), while Sporidiobolus sp. and Bullera alba, dominant on faba bean (Vicia faba), were isolated in the U.K. Dose responses were determined separately for each yeast. UV-B reduced colony forming units (due to cell mortality or inactivation) and colony size (due to reduced multiplication) of all yeasts. The LD 50 values and doses causing 50% reduction of cells per colony were higher for SL isolates than U.K. isolates. Results indicated that each yeast is somewhat vulnerable to UV-B doses representative of its natural habitat. The relative insensitivity of SL isolates was shown when SL and U.K. isolates were irradiated simultaneously with the same dose of UV-B. Of the two U.K. yeasts, B. alba was significantly more sensitive than Sporidiobolus sp. to UV-B. Except for R. mucilaginosa from SL, all yeasts demonstrated some photorepair in the presence of white light. White light provided relatively little protection for the U.K. isolate of Sporidiobolus sp. although it allowed increased colony size. The spectral responses of Sporidiobolus sp. (U.K.) and of B. alba (U.K.) were broadly similar. Wavelengths longer than 320 nm had no measurable effect on colony forming units. However, colony survival was significantly reduced at 310 nm and all shorter wavebands. No colonies were counted at 290 nm or below. (author)

  3. Aerosol effects on UV radiation

    International Nuclear Information System (INIS)

    Koepke, P.; Reuder, J.; Schwander, H.

    2000-01-01

    The reduction of erythemally weighted UV-irradiance (given as UV index, UVI) due to aerosols is analyzed by variation of the tropospheric particles in a wide, but realistic range. Varied are amount and composition of the particles and relative humidity and thickness of the mixing layer. The reduction of UVI increases with aerosol optical depth and the UV change is around 10% for a change aerosol optical depth from 0.25 to 0.1 and 0.4 respectively. Since both aerosol absorption and scattering are of relevance, the aerosol effect depends besides total aerosol amount on relative amount of soot and on relative humidity

  4. UV radiation-induced photochemical damage of tryptophan in peptides, proteins and ocular lenses

    International Nuclear Information System (INIS)

    Hibbard, L.B.

    1985-01-01

    These studies were undertaken to investigate the possible involvement of the amino acid tryptophan in the near-ultraviolet radiation-induced photochemical alteration of peptides and proteins and the role tryptophan photolysis plays in ocular lens damage. Sample irradiations were performed to determine if tryptophan photolysis occurs with radiation in the UV-A region in comparison to photolysis induced by wavelengths in the normal absorption band of the amino acid (UV-B). Photolysis studies were carried out on free tryptophan and two dipeptides, tryptophyglycine and glycyltryptophan, in aqueous solutions at different pH values in the range 4.5-10.0 under aerated or anaerobic conditions. Rates of photolysis of these 290 nm-irradiated compounds, detected by observing tryptophan fluorescence intensity loss during irradiation, were compared and significant differences were observed for each compound which varied with pH and oxygen environment. Another series of experiments examined the photolysis of tryptophan residues in lens proteins in whole rat lenses induced by 290 nm and 298 nm dye laser radiation. Tryptophan residue photolysis was, once again, monitored by loss in tryptophan fluorescence intensity. A relationship was derived between tryptophan loss and photoproduct buildup during irradiation

  5. Study of Leaf Metabolome Modifications Induced by UV-C Radiations in Representative Vitis, Cissus and Cannabis Species by LC-MS Based Metabolomics and Antioxidant Assays

    Directory of Open Access Journals (Sweden)

    Guillaume Marti

    2014-09-01

    Full Text Available UV-C radiation is known to induce metabolic modifications in plants, particularly to secondary metabolite biosynthesis. To assess these modifications from a global and untargeted perspective, the effects of the UV-C radiation of the leaves of three different model plant species, Cissus antarctica Vent. (Vitaceae, Vitis vinifera L. (Vitaceae and Cannabis sativa L. (Cannabaceae, were evaluated by an LC-HRMS-based metabolomic approach. The approach enabled the detection of significant metabolite modifications in the three species studied. For all species, clear modifications of phenylpropanoid metabolism were detected that led to an increased level of stilbene derivatives. Interestingly, resveratrol and piceid levels were strongly induced by the UV-C treatment of C. antarctica leaves. In contrast, both flavonoids and stilbene polymers were upregulated in UV-C-treated Vitis leaves. In Cannabis, important changes in cinnamic acid amides and stilbene-related compounds were also detected. Overall, our results highlighted phytoalexin induction upon UV-C radiation. To evaluate whether UV-C stress radiation could enhance the biosynthesis of bioactive compounds, the antioxidant activity of extracts from control and UV-C-treated leaves was measured. The results showed increased antioxidant activity in UV-C-treated V. vinifera extracts.

  6. The Skin Microbiome: Is It Affected by UV-induced Immune Suppression?

    Science.gov (United States)

    Patra, VijayKumar; Byrne, Scott N.; Wolf, Peter

    2016-01-01

    Human skin apart from functioning as a physical barricade to stop the entry of pathogens, also hosts innumerable commensal organisms. The skin cells and the immune system constantly interact with microbes, to maintain cutaneous homeostasis, despite the challenges offered by various environmental factors. A major environmental factor affecting the skin is ultraviolet radiation (UV-R) from sunlight. UV-R is well known to modulate the immune system, which can be both beneficial and deleterious. By targeting the cells and molecules within skin, UV-R can trigger the production and release of antimicrobial peptides, affect the innate immune system and ultimately suppress the adaptive cellular immune response. This can contribute to skin carcinogenesis and the promotion of infectious agents such as herpes simplex virus and possibly others. On the other hand, a UV-established immunosuppressive environment may protect against the induction of immunologically mediated skin diseases including some of photodermatoses such as polymorphic light eruption. In this article, we share our perspective about the possibility that UV-induced immune suppression may alter the landscape of the skin’s microbiome and its components. Alternatively, or in concert with this, direct UV-induced DNA and membrane damage to the microbiome may result in pathogen associated molecular patterns (PAMPs) that interfere with UV-induced immune suppression. PMID:27559331

  7. The skin microbiome: Is it affected by UV-induced immune suppression?

    Directory of Open Access Journals (Sweden)

    Vijaykumar Patra

    2016-08-01

    Full Text Available Human skin apart from functioning as a physical barricade to stop the entry of pathogens, also hosts innumerable commensal organisms. The skin cells and the immune system constantly interact with microbes, to maintain cutaneous homeostasis, despite the challenges offered by various environmental factors. A major environmental factor affecting the skin is ultraviolet radiation UV-R from sunlight. UV-R is well known to modulate the immune system, which can be both beneficial and deleterious. By targeting the cells and molecules within skin, UV-R can trigger the production and release of antimicrobial peptides (AMPs, affect the innate immune system and ultimately suppress the adaptive cellular immune response. This can contribute to skin carcinogenesis and the promotion of infectious agents such as herpes simplex virus and possibly others. On the other hand, a UV-established immunosuppressive environment may protect against the induction of immunologically mediated skin diseases including some of photodermatoses such as polymorphic light eruption. In this article, we share our perspective about the possibility that UV-induced immune suppression may alter the landscape of the skin's microbiome and its components. Alternatively, or in concert with this, direct UV-induced DNA and membrane damage to the microbiome may result in pathogen associated molecular patterns (PAMPs that interfere with UV-induced immune suppression.

  8. Effects of enhanced ultraviolet-B radiation, water deficit, and their combination on UV-absorbing compounds and osmotic adjustment substances in two different moss species.

    Science.gov (United States)

    Hui, Rong; Zhao, Ruiming; Song, Guang; Li, Yixuan; Zhao, Yang; Wang, Yanli

    2018-05-01

    A simulation experiment was conducted to explore the influence of enhanced ultraviolet-B (UV-B) radiation, water deficit, and their combination on UV-absorbing compounds and osmotic adjustment substances of mosses Bryum argenteum and Didymodon vinealis isolated from biological soil crusts (BSCs) growing in a revegetated area of the Tengger Desert, China. Four levels of UV-B radiation and two gradients of water regime were employed. Compared with their controls, amounts of total flavonoids, chlorophyll, carotenoids, soluble sugars, and soluble proteins significantly decreased (p argenteum, D. vinealis was more resistant to enhanced UV-B and water deficit singly and in combination. These results suggest that the damage of enhanced UV-B on both species might be alleviated by water deficit. This alleviation is important for understanding the response of BSCs to UV-B radiation in future global climate change. This also provides novel insights into assessment damages of UV-B to BSC stability in arid and semiarid regions.

  9. Leaf surface wax is a source of plant methane formation under UV radiation and in the presence of oxygen

    DEFF Research Database (Denmark)

    Bruhn, Dan; Mikkelsen, Teis Nørgaard; Rolsted, M. M. M.

    2014-01-01

    to this, we demonstrated that the UV radiation-induced CH4 emission is independent of leaf area index above unity. Further, we observed that the presence of O2 in the atmosphere was necessary for achieving the highest rates of CH4 emission. Methane formation from leaf surface wax is supposedly a two...... investigated the potential of the leaf surface wax itself as a source of UV radiationinduced leaf aerobic CH4 formation. Isolated leaf surface wax emitted CH4 at substantial rates in response to UV radiation. This discovery has implications for how the phenomenon should be scaled to global levels. In relation...

  10. Biological monitoring of radiation exposure

    Science.gov (United States)

    Horneck, G.

    1998-11-01

    Complementary to physical dosimetry, biological dosimetry systems have been developed and applied which weight the different components of environmental radiation according to their biological efficacy. They generally give a record of the accumulated exposure of individuals with high sensitivity and specificity for the toxic agent under consideration. Basically three different types of biological detecting/monitoring systems are available: (i) intrinsic biological dosimeters that record the individual radiation exposure (humans, plants, animals) in measurable units. For monitoring ionizing radiation exposure, in situ biomarkers for genetic (e.g. chromosomal aberrations in human lymphocytes, germ line minisatellite mutation rates) or metabolic changes in serum, plasma and blood (e.g. serum lipids, lipoproteins, lipid peroxides, melatonin, antibody titer) have been used. (ii) Extrinsic biological dosimeters/indicators that record the accumulated dose in biological model systems. Their application includes long-term monitoring of changes in environmental UV radiation and its biological implications as well as dosimetry of personal UV exposure. (iii) Biological detectors/biosensors for genotoxic substances and agents such as bacterial assays (e.g. Ames test, SOS-type test) that are highly sensitive to genotoxins with high specificity. They may be applicable for different aspects in environmental monitoring including the International Space Station.

  11. Responses of photosynthetic properties and chloroplast ultrastructure of two moss crusts from a desert biological soil crust to supplementary UV-B radiation

    Science.gov (United States)

    Hui, Rong; Li, Xinrong; Zhao, Yang; Pan, Yanxia

    2016-04-01

    Our understanding of plant responses to supplementary ultraviolet-B (UV-B) radiation due to stratospheric ozone depletion has improved over recent decades. However, research on biological soil crusts (BSCs) is scarce and it remains controversial. Laboratory studies were conducted to investigate the influence of UV-B radiation on the Bryum argenteum and Didymodon vinealis isolated from BSCs, which are both dominant species in moss crusts found within patches of shrubs and herbs in the Tengger Desert of northern China. The aim of the current work was to evaluate whether supplementary UV-B radiation affected photosynthetic properties and chloroplast ultrastructure of two moss crusts and whether response differences were observed between the crusts. Four levels of UV-B radiation of 2.75 (control), 3.08, 3.25, and 3.41 W m-2 was achieved using fluorescence tube systems for 10 days, simulating 0, 6, 9, and 12% of stratospheric ozone at the latitude of Shapotou, respectively. We measured photosynthetic apparatus as assessed by chlorophyll a fluorescence parameters, photosynthetic pigment contents, and observations of chloroplast ultrastructure. Additionally, soluble proteins and UV-B absorbing compounds were simultaneously investigated. The results of this study showed that chlorophyll a fluorescence parameters (i.e., the maximal quantum yield of PSII photochemistry, the effective quantum yield of PSII photochemistry, and photochemical quenching coefficient), photosynthetic pigment contents, soluble protein contents, total flavonoid contents and the ultrastructure were negatively influenced by elevated UV-B radiation and the degree of detrimental effects significantly increased with the intensity of UV-B radiation. Moreover, results demonstrated that the negative effects on photosynthesis and chloroplast ultrastructure were more serious in B. argenteum than that in D. vinealis. These results may not only provide a potential mechanism for supplemental UV-B effects on

  12. Luminescent emission of LiF: Mg, Ti exposed to UV radiation; Emision luminiscente del LiF: Mg, Ti expuesto a la radiacion UV

    Energy Technology Data Exchange (ETDEWEB)

    Estrada G, A. [Estudiante de Facultad de Ciencias, UNAM, Circuito Exterior, 04500 Mexico D.F. (Mexico); Castano M, V.M. [Centro de Fisica Aplicada y Tecnologia Avanzada, UNAM, Campus Juriquilla, Queretaro (Mexico); Cruz Z, E.; Garcia F, F. [Instituto de Ciencias Nucleares UNAM, A.P. 70-543 Mexico D.F. (Mexico)

    2002-07-01

    It was investigated the luminescent emission stimulated by heat (Tl) of LiF: Mg, Ti crystals which were exposed to UV radiation coming from a mercury lamp. Since this crystal depends on the thermal history, it has been used a thermal treatment consisting of a baking at 380 C during one hour for each reading and they were irradiated with UV. The brilliance curves between 5 and 840 minutes of exposure in the face of UV light were obtained. An important loss in the response, starting from 150 minutes of irradiation was observed. Also the relative intensity of the brilliance curve decay when the crystals being stored in darkness and room temperature conditions, which is according to the results in the literature about. (Author)

  13. Development of a low cost UV index datalogger and comparison between UV index sensors

    Science.gov (United States)

    Gomes, L. M.; Ventura, L.

    2018-02-01

    Ultraviolet radiation (UVR) is the part of radiation emitted by the Sun, with range between 280 nm and 400 nm, and that reaches the Earth's surface. The UV rays are essential to the human because it stimulates the production of vitamin D but this radiation may be related to several health problems, including skin cancer and ocular diseases like pterygium, photokeratitis, cataract and more. To inform people about UV radiation, it is adopted the Ultraviolet Index (UVI). This UVI consists in a measure of solar UV radiation level, which contributes to cause sunburn on skin, also known as Erythema, and is indicated as an integer number between 1 and 14, associated to categories from low to extreme respectively. The aim of this work was to develop a low cost UVI datalogger capable of measuring three different UVI sensors simultaneously, record their data with timestamp and serve the measures online through a dedicated server, so general public can access their data and see the current UV radiation conditions. We also compared three different UVI sensors (SGlux UV cosine, Skye SKU440 and SiLabs SI1145) between them and with meteorological models during a period of months to verify their compliance. With five months data, we could verify the sensors working characteristics and decide which among them are the most suitable for research purposes.

  14. MEASUREMENT OF SURFACE SOLAR UV-B RADIATION AT TROPICAL COASTAL STATION BAKKHALI IN WEST BENGAL, INDIA

    OpenAIRE

    R. BHATTACHARYA; A. BHOUMICK

    2012-01-01

    Surface solar ultraviolet irradiance has been measured at Bakkhali (21.8ºN, 87.8ºE), a tropical rural station on the coast of Bay of Bengal, India in West Bengal. The measurements show a remarkable variation in UV-B load exists with a peak value at noon. The blockage of direct UV radiation in mangrove forest of costal site appears low when compared with UV load beneath the multiple trees of Mangifera indica in an inland site of Kalyani (22058' N, 88028' E), West Bengal. Mangrove forests have ...

  15. Environmental Radiation Monitoring Around the Nuclear Facilities

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Geun Sik; Lee, Chang Woo

    2008-05-15

    Environmental Radiation Monitoring was carried out with measurement of environment. radiation and environmental radioactivity analysis on the sites of KAERI nuclear facilities and Seoul Research Reactors and their environments. The average level of environmental radiation dose measured by an ERM and the accumulated radiation dose by a TLD were almost same level compared with the previous years. The activity of gross {alpha} and gross {beta}, Tritium, Uraniu and Strontium in environmental samples showed a environmental level. The radioactivities of most {gamma}-radionuclides in air particulate, surface water and ground water were less than MDA except {sup 40}K or {sup 7}Be which are natural radionuclides. However, not only {sup 40}K or {sup 7}Be but also {sup 137}Cs were detected at the background level in surface soil, discharge sediment and fallout or pine needle.

  16. Exposure to UV radiation and human health

    Science.gov (United States)

    Kimlin, Michael G.

    2005-08-01

    This paper will overview the significant issues facing researchers in relating the impact of exposure to sunlight and human health. Exposure to solar ultraviolet radiation is the major causative factor in most sun-related skin and eye disorders, however, very little is known quantitatively about human UV exposures. Interestingly, human exposure to sunlight also has a nutritional impact, namely the development of pre-Vitamin D, which is an important nutrient in bone health. New research suggest that low vitamin D status may be a causative factor in the development of selective types of cancer and autoimminue diseases, as well as a contributing factor in bone health. The 'health duality' aspect of sunlight exposure is an interesting and controversial topic that is a research focus of Kimlin's research group.

  17. Impact of ultraviolet-B radiation on growth and development of the plants - literature review

    International Nuclear Information System (INIS)

    Zuk-Golaszewska, K.

    2003-01-01

    Paper presents a review of the recent literature dealing with the UV-B radiation, its effect on physiological processes of plant growth and development, cellular changes, concentration of chemical compounds and changes in morphological plant traits. The reasons of increasing UV-B radiation level are rapidly developing civilization and decreasing of ozone layer. It was stated that the plant reaction to UV-B irradiation depends on plant species and environmental conditions. Destructive effects of UV-B radiation to plants may be - to some extent - neutralized by defence mechanisms, a form of specific plant adaptation to stress, however, under conditions of strong UV-B irradiation such mechanisms are not sufficient

  18. Polymer-layered silicate nano composite by UV-radiation curing: an original synthesis

    International Nuclear Information System (INIS)

    Keller, L.; Decker, C.; Zahouily, K.; Miehe-Brendle, J.; Le Meins, J.M.

    2004-01-01

    Full text.Because of the many hopes which they raise, the nano composite materials are the subject of an increasing number of scientific publications. Indeed, the intimate association of a polymer matrix and silicate nano-platelets leads to the formation of materials having mechanical and barriers properties improved (fire, gas, humidity...). A literature survey shows that these materials are generally produced by a thermal polymerization, which presents two major disadvantages: the use of organic solvents and a great consumption of energy. To overcome such limitations, photo initiated polymerization was chosen to synthesize nano composite materials. By this technology, called UV radiation curing, a solvent-free resin is transformed within seconds into a solid polymer upon exposure to UV-radiation at ambient temperature. The principal objective of this study was to develop photopolymerizable systems with clay particles having a layer structure (phyllosilicates). The clay mineral was made organophilic by treatment with an alkylammonium salt to allow the acrylate resin to penetrate into the expanded galleries. A morphological characterization of the materials obtained was carried out by X-rays diffraction and electronic microscopy transmission. The polymerization of the various resins under the UV exposure was followed in situ by using the real-time infrared spectroscopy (RT-FTIR) and attenuated total reflection (ATR). The results obtained show that the presence of the organo clay does not modify much the polymerization kinetics. The nano composite material thus obtained is transparent, insoluble in the organic solvents and presents improved mechanical properties, compared to the neat resin and the micro composite, for a load factor ranging between 2 and 5%wt. The addition of nanoparticles also makes it possible to reduce efficiently the brightness of coatings UV and finally confers to this material barriers properties higher than that of the photo crosslinked

  19. Soybean resistance to stink bugs (Nezara viridula and Piezodorus guildinii) increases with exposure to solar UV-B radiation and correlates with isoflavonoid content in pods under field conditions.

    Science.gov (United States)

    Zavala, Jorge A; Mazza, Carlos A; Dillon, Francisco M; Chludil, Hugo D; Ballaré, Carlos L

    2015-05-01

    Solar UV-B radiation (280-315 nm) has a significant influence on trophic relationships in natural and managed ecosystems, affecting plant-insect interactions. We explored the effects of ambient UV-B radiation on the levels of herbivory by stink bugs (Nezara viridula and Piezodorus guildinii) in field-grown soybean crops. The experiments included two levels of UV-B radiation (ambient and attenuated UV-B) and four soybean cultivars known to differ in their content of soluble leaf phenolics. Ambient UV-B radiation increased the accumulation of the isoflavonoids daidzin and genistin in the pods of all cultivars. Soybean crops grown under attenuated UV-B had higher numbers of unfilled pods and damaged seeds than crops grown under ambient UV-B radiation. Binary choice experiments with soybean branches demonstrated that stink bugs preferred branches of the attenuated UV-B treatment. We found a positive correlation between percentage of undamaged seeds and the contents of daidzin and genistin in pods. Our results suggest that constitutive and UV-B-induced isoflavonoids increase plant resistance to stink bugs under field conditions. © 2014 John Wiley & Sons Ltd.

  20. Surface Coating of Musa Brachycarpa Trunk Using UV-Radiation

    International Nuclear Information System (INIS)

    Danu, Sugiarto; Sumarni, Anik; Nurhadi, Agus; Puspita, Rita

    2000-01-01

    An experiment on UV-curing of surface coating of Musa brachycarpa was carried out using urethane acrylate polymer films. Radiation curable material was the mixture of urethane acrylate resin, tripropylene glycol diacrylate monomer (TPGDA) and radical photo initiator of 2,2-dimethyl -2-hydroxy acetophenone. The TPGDA concentrations in the mixture with urethane acrylate resin were 60; 70 and 80% weight, Whereas concentrations of photo initiator were varied at the level 1.5:2.0 and 2.5% by weight based on resin and monomer mixture. Irradiation was conducted by using 80 Watt/cm intensity UV-light at the conveyor speed of 2: 3 and 4 m/min. Analysis and film properties observed were IR spectrum, gel fraction, hardness, abrasion resistance, glossy and chemical, solvent and stain resistances. Films have good resistances against 1% sodium carbonate, 5% acetic acid, 50% alcohol, thinner and red, blue and black permanent marker, except against 10% sodium hydroxide and 10% sulfuric acid. Optimum condition was achieved at the photo initiator concentration level of 2% and conveyor speed of 3 m/min

  1. Beneficial effects of enhanced UV-B radiation under field conditions: improvement of needle water relations and survival capacity of Pinus pinea L. seedlings during the dry Mediterranean summer

    International Nuclear Information System (INIS)

    Manetas, Y.; Petropoulou, Y.; Stamatakis, K.; Nikolopoulos, D.; Levizou, E.; Psaras, G.; Karabourniotis, G.

    1997-01-01

    The possible mechanism(s) by which supplemental UV-B radiation alleviates the adverse effects of summer drought in Mediterranean pines (Petropoulou et al. 1995) were investigated with seedlings of Pinus pinea. Plants received ambient or ambient plus supplemental UV-B radiation (biologically equivalent to a 15% ozone depletion over Patras, 38.3° N, 29.1° E) and natural precipitation or additional irrigation. Treatments started on 1 February, 1994 and lasted up to the end of the dry period (29 September). In well-watered plants, UV-B radiation had no influence on photosystem II photochemical efficiency and biomass accumulation. Water stressed plants suffered from needle loss and reduced photosystem II photochemical efficiency during the summer. These symptoms, however, were less pronounced in plants receiving supplemental UV-B radiation, resulting in higher total biomass at plant harvest. Laboratory tests showed that enhanced UV-B radiation did not improve the tolerance of photosystem II against drought, high light, high temperature and oxidative stress. Enhanced UV-B radiation, however, improved the water economy of water stressed plants, as judged by measurements of needle relative water content. In addition, it caused an almost two-fold increase of cuticle thickness. No such UV-B radiation effects were observed in well-watered pines. The results indicate that the combination of water stress and UV-B radiation may trigger specific responses, enabling the plants to avoid excessive water loss and, thereby, maintain a more efficient photosynthetic apparatus during the summer. The extent of this apparently positive UV-B radiation effect would depend on the amount of summer precipitation. Abbreviations: DW – dry weight, F v /F m – ratio of variable to maximum fluorescence, A 300 – absorbance at 300 nm, PAR – photosynthetically active radiation, PS II – photosystem II, RWC – relative water content, TCA – trichloroacetic acid, UV-B BE – biologically

  2. UV laser radiation alters the embryonic protein profile of adrenal-kidney-gonadal complex and gonadal differentiation in the lizard, Calotes Versicolor.

    Science.gov (United States)

    Khodnapur, Bharati S; Inamdar, Laxmi S; Nindi, Robertraj S; Math, Shivkumar A; Mulimani, B G; Inamdar, Sanjeev R

    2015-02-01

    To examine the impact of ultraviolet (UV) laser radiation on the embryos of Calotes versicolor in terms of its effects on the protein profile of the adrenal-kidney-gonadal complex (AKG), sex determination and differentiation, embryonic development and hatching synchrony. The eggs of C. versicolor, during thermo-sensitive period (TSP), were exposed to third harmonic laser pulses at 355 nm from a Q-switched Nd:YAG laser for 180 sec. Subsequent to the exposure they were incubated at the male-producing temperature (MPT) of 25.5 ± 0.5°C. The AKG of hatchlings was subjected to protein analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and to histology. The UV laser radiation altered the expression of the protein banding pattern in the AKG complex of hatchlings and it also affected the gonadal sex differentiation. SDS-PAGE of AKG of one-day-old hatchlings revealed a total of nine protein bands in the control group whereas UV laser irradiated hatchlings expressed a total of seven protein bands only one of which had the same Rf as a control band. The UV laser treated hatchlings have an ovotestes kind of gonad exhibiting a tendency towards femaleness instead of the typical testes. It is inferred that 355 nm UV laser radiation during TSP induces changes in the expression of proteins as well as their secretions. UV laser radiation had an impact on the gonadal differentiation pathway but no morphological anomalies were noticed.

  3. Surface coatings of unsaturated polyester resin Kamper wood (Dry obalan ops spp.) by using UV radiation

    International Nuclear Information System (INIS)

    Sugiarto Danu; Yusuf Sudo Hadi; Novi Eka Putri

    1999-01-01

    Kamper wood (Dryobalanops spp.) has high contribution in wood working industry and most of them need surface coating process. Radiation curing of surface coating, especially the use of ultra-violet (UV) light have potential to give contribution in the wood finishing. The experiment on surface coating of kamper wood has been conducted by using UV-radiation. Unsaturated polyester resin with the commercial name of Yucalac type 157 was used as coating materials after being added with styrene monomer, some fillers and radical photoinitiator of 2-hydroxy-2-2-methyl-l- phenyl propanone. Four photoinitiator concentration levels of 1.5 ; 2 ; 2.5 and 3 % by weight of resin were used. The coating materials were coated onto the wood panel samples by using high pressure sprayer. The wood samples were then exposed to irradiation by using 80 Watts/cm UV-source with variable conveyor speed of 3 ; 4 ; 5 and 5.8 m/min. Formulation of coating materials, pendulum hardness, adhesion, and gloss of cured films were evaluated

  4. Study and characterization of phosphors excited in the V UV and UV range by the synchrotron radiation

    International Nuclear Information System (INIS)

    Gerard, I.

    1993-01-01

    A characterization tool using synchrotron radiation as a light source to record excitation spectra of the visible luminescence of phosphors induced by photons in the V UV and UV range, at several temperatures (10 to 300 K), is developed. The absorption and deexcitation mechanisms in Y F 3 , La F 3 and Th F 4 matrices doped with Eu 3+ , Tb 3+ , Dy 3+ and Er 3+ ions and polluted with oxygen ions, are examined; charge transfer bands appear clearly. The 4 f n to 4 f n-1 5 d transition bands are also observed on the excitation spectra of the visible luminescence of these compounds and two processes are proposed to interpret the energy relaxation. In order to determine the candidates for the color plasma display panel, measurements of luminous and external quantum yields for efficient phosphors are carried out. The Y F 3 :Eu 3+ compound is shown as a good candidate for the red emission in color plasma display panels

  5. Dosimetric characterization of chemical-vapor-deposited diamond film irradiated with UV and beta radiation

    Science.gov (United States)

    Meléndrez, R.; Chernov, V.; Pedroza-Montero, M.; Barboza-Flores, M.

    2003-03-01

    Diamond is an excellent prospect for clinical radiation dosimetry due to its tissue-equivalence properties and being chemically inert. The use of diamond in radiation dosimetry has been halted by the high market price; although recently the capability of growing high quality polycrystalline has renewed the interest in using diamond films as detectors and dosimeters. In the present work we have characterized the dosimetric properties of diamond films synthesized by using chemical vapor deposition. The thermoluminescence (TL) of UV and beta exposed samples shows a glow curve composed of at least four peaks; one located around 587 K presents excellent TL properties suitable for dosimetric applications with ionizing and non ionizing radiation. The TL excitation spectrum exhibits maximum TL efficiency at 220 nm. The samples show regions of linear as well as supralinear behavior as a function or irradiation dose. The linear dose dependence was found for up to sixteen minutes of UV irradiation and 300 Gy for beta irradiated samples. The activation energy and the frequency factor were determined and found in the range of 0.32 - 0.89 eV and 1.1x10^2 - 2x10^8s_-1, respectively. The observed TL performance is reasonable appropriate to justify further investigation of diamond films as radiation dosimeters.

  6. Effects of silicon application on diurnal variations of physiological properties of rice leaves of plants at the heading stage under elevated UV-B radiation

    Science.gov (United States)

    Lou, Yun-sheng; Wu, Lei; Lixuan, Ren; Meng, Yan; Shidi, Zhao; Huaiwei, Zhu; Yiwei, Zhang

    2016-02-01

    We investigated the effects of silicon (Si) application on diurnal variations of photosynthetic and transpiration physiological parameters in potted rice ( Oryza sativa L. cv Nanjing 45) at the heading stage. The plants were subjected to two UV-B radiation levels, i.e., reference UV-B (A, ambient, 12.0 kJ m-2 day-1) and elevated UV-B radiation (E, a 20 % higher dose of UV-B than the reference, 14.4 kJ m-2 day-1), and four Si application levels, i.e., Si0 (no silicon supplementation, 0 kg SiO2 ha-1), Si1 (sodium silicate, 100 kg SiO2 ha-1), Si2 (sodium silicate, 200 kg SiO2 ha-1), and Si3 (slag silicon fertilizer, 200 kg SiO2 ha-1). Compared with the reference, elevated UV-B radiation decreased the diurnal mean values of the net photosynthetic rate ( Pn), intercellular carbon dioxide (CO2) concentration ( Ci), transpiration rate ( Tr), stomatal conductivity ( Gs), and water use efficiency (WUE) by 11.3, 5.5, 10.4, 20.3, and 6.3 %, respectively, in plants not supplemented with silicon (Si0), and decreased the above parameters by 3.8-5.5, 0.7-4.8, 4.0-8.7, 7.4-20.2, and 0.7-5.9 %, respectively, in plants treated with silicon (Si1, Si2, and Si3), indicating that silicon application mitigates the negative effects of elevated UV-B radiation. Under elevated UV-B radiation, silicon application (Si1, Si2, and Si3) increased the diurnal mean values of Pn, Ci, Gs, and WUE by 16.9-28.0, 3.5-14.3, 16.8-38.7, and 29.0-51.2 %, respectively, but decreased Tr by 1.9-10.8 %, compared with plants not treated with silicon (E+Si0), indicating that silicon application mitigates the negative effects of elevated UV-B radiation by significantly increasing the P n, C i, G s, and WUE and decreasing the T r of rice. Evident differences existed in mitigating the depressive effects of elevated UV-B radiation on diurnal variations of physiological parameters among different silicon application treatments, exhibiting as Si3>Si2>Si1>Si0. In addition to recycling steel industrial wastes, the

  7. Effects of silicon application on diurnal variations of physiological properties of rice leaves of plants at the heading stage under elevated UV-B radiation.

    Science.gov (United States)

    Lou, Yun-sheng; Wu, Lei; Lixuan, Ren; Meng, Yan; Shidi, Zhao; Huaiwei, Zhu; Yiwei, Zhang

    2016-02-01

    We investigated the effects of silicon (Si) application on diurnal variations of photosynthetic and transpiration physiological parameters in potted rice (Oryza sativa L. cv Nanjing 45) at the heading stage. The plants were subjected to two UV-B radiation levels, i.e., reference UV-B (A, ambient, 12.0 kJ m(-2) day(-1)) and elevated UV-B radiation (E, a 20% higher dose of UV-B than the reference, 14.4 kJ m(-2) day(-1)), and four Si application levels, i.e., Si0 (no silicon supplementation, 0 kg SiO2 ha(-1)), Si1 (sodium silicate, 100 kg SiO2 ha(-1)), Si2 (sodium silicate, 200 kg SiO2 ha(-1)), and Si3 (slag silicon fertilizer, 200 kg SiO2 ha(-1)). Compared with the reference, elevated UV-B radiation decreased the diurnal mean values of the net photosynthetic rate (Pn), intercellular carbon dioxide (CO2) concentration (Ci), transpiration rate (Tr), stomatal conductivity (Gs), and water use efficiency (WUE) by 11.3, 5.5, 10.4, 20.3, and 6.3%, respectively, in plants not supplemented with silicon (Si0), and decreased the above parameters by 3.8-5.5, 0.7-4.8, 4.0-8.7, 7.4-20.2, and 0.7-5.9%, respectively, in plants treated with silicon (Si1, Si2, and Si3), indicating that silicon application mitigates the negative effects of elevated UV-B radiation. Under elevated UV-B radiation, silicon application (Si1, Si2, and Si3) increased the diurnal mean values of Pn, Ci, Gs, and WUE by 16.9-28.0, 3.5-14.3, 16.8-38.7, and 29.0-51.2%, respectively, but decreased Tr by 1.9-10.8%, compared with plants not treated with silicon (E+Si0), indicating that silicon application mitigates the negative effects of elevated UV-B radiation by significantly increasing the P n, C i, G s, and WUE and decreasing the T r of rice. Evident differences existed in mitigating the depressive effects of elevated UV-B radiation on diurnal variations of physiological parameters among different silicon application treatments, exhibiting as Si3>Si2>Si1>Si0. In addition to recycling steel industrial wastes, the

  8. Mutation induction in haploid yeast after split-dose radiation exposure. II. Combination of UV-irradiation and X-rays.

    Science.gov (United States)

    Keller, B; Zölzer, F; Kiefer, J

    2004-01-01

    Split-dose protocols can be used to investigate the kinetics of recovery from radiation damage and to elucidate the mechanisms of cell inactivation and mutation induction. In this study, a haploid strain of the yeast, Saccharomyces cerevisiae, wild-type with regard to radiation sensitivity, was irradiated with 254-nm ultraviolet (UV) light and then exposed to X-rays after incubation for 0-6 hr. The cells were incubated either on nutrient medium or salt agar between the treatments. Loss of reproductive ability and mutation to canavanine resistance were measured. When the X-ray exposure immediately followed UV-irradiation, the X-ray survival curves had the same slope irrespective of the pretreatment, while the X-ray mutation induction curves were changed from linear to linear quadratic with increasing UV fluence. Incubations up to about 3 hr on nutrient medium between the treatments led to synergism with respect to cell inactivation and antagonism with respect to mutation, but after 4-6 hr the two treatments acted independently. Incubation on salt agar did not cause any change in the survival curves, but there was a strong suppression of X-ray-induced mutation with increasing UV fluence. On the basis of these results, we suggest that mutation after combined UV and X-ray exposure is affected not only by the induction and suppression of DNA repair processes, but also by radiation-induced modifications of cell-cycle progression and changes in the expression of the mutant phenotype. Copyright 2004 Wiley-Liss, Inc.

  9. Antioxidant enzymes expression in Pseudomonas aeruginosa exposed to UV-C radiation.

    Science.gov (United States)

    Salma, Kloula Ben Ghorbal; Lobna, Maalej; Sana, Khefacha; Kalthoum, Chourabi; Imene, Ouzari; Abdelwaheb, Chatti

    2016-07-01

    It was well known that, UV-C irradiation increase considerably the reactive oxygen species (ROS) levels in eukaryotic and prokaryotic organisms. In the enzymatic ROS-scavenging pathways, superoxide dismutase (SOD), Catalase (CAT), and peroxidase (POX) were developed to deal with oxidative stress. In this study, we investigated the effects of UV-C radiations on antioxidant enzymes (catalase, superoxide dismutase, and peroxidases) expression in Pseudomonas aeruginosa. Catalase, superoxide dismutase, and peroxidases activities were determined spectrophotometrically. Isozymes of superoxide dismutase were revealed by native gel activity staining method. Lipid peroxidation was determined by measuring malondialdehyde formation. Our results showed that superoxide dismutase, catalase and peroxidase activities exhibited a gradual increase during the exposure time (30 min). However, the superoxide dismutase activity was maximized at 15 min. Native gel activity staining assays showed the presence of three superoxide dismutase isozymes. The iron-cofactored isoform activity was altered after exposure to UV-C stress. These finding suggest that catalase and peroxidase enzymes have the same importance toward UV-C rays at shorter and longer exposure times and this may confer additional protection to superoxide dismutase from damage caused by lipid peroxidation. Moreover, our data demonstrate the significant role of the antioxidant system in the resistance of this important human pathogen. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Experimental Evolution of UV-C Radiation Tolerance: Emergence of Adaptive and Non-Adaptive Traits in Escherichia coli Under Differing Flux Regimes

    Science.gov (United States)

    Moffet, A.; Okansinski, A.; Sloan, C.; Grace, J. M.; Paulino-Lima, I. G.; Gentry, D.; Rothschild, L. J.; Camps, M.

    2014-12-01

    High-energy ultraviolet (UV-C) radiation is a significant challenge to life in environments such as high altitude areas, the early Earth, the Martian surface, and space. As UV-C exposure is both a selection pressure and a mutagen, adaptation dynamics in such environments include a high rate of change in both tolerance-related and non-tolerance-related genes, as well changes in linkages between the resulting traits. Determining the relationship between the intensity and duration of the UV-C exposure, mutation rate, and emergence of UV-C resistance will inform our understanding of both the emergence of radiation-related extremophily in natural environments and the optimal strategies for generating artificial extremophiles. In this study, we iteratively exposed an Escherichia colistrain to UV-C radiation of two different fluxes, 3.3 J/m^2/s for 6 seconds and 0.5 J/m^2/s for 40 seconds, with the same overall fluence of 20 J/m^2. After each iteration, cells from each exposure regime were assayed for increased UV-C tolerance as an adaptive trait. The exposed cells carried a plasmid bearing a TEM beta-lactamase gene, which in the absence of antibiotic treatment is a neutral reporter for mutagenesis. Sequencing of this gene allowed us to determine the baseline mutation frequency for each flux. As an additional readout for adaptation, the presence of extended-spectrum beta-lactamase mutations was tested by plating UV-exposed cultures in cefotaxime plates. We observed an increase of approximately one-million-fold in UV-C tolerance over seven iterations; no significant difference between the two fluxes was found. Future work will focus on identifying the genomic changes responsible for the change in UV-C tolerance; determining the mechanisms of the emerged UV-C tolerance; and performing competition experiments between the iteration strains to quantify fitness tradeoffs resulting from UV-C adaptation.

  11. Induction of wound-periderm-like tissue in Kalanchoe pinnata (Lam.) Pers. (Crassulaceae) leaves as a defence response to high UV-B radiation levels.

    Science.gov (United States)

    Nascimento, Luana Beatriz dos Santos; Moreira, Nattacha dos Santos; Leal-Costa, Marcos Vinícius; Costa, Sônia Soares; Tavares, Eliana Schwartz

    2015-10-01

    UV-B radiation can be stressful for plants and cause morphological and biochemical changes. Kalanchoe pinnata is a CAM leaf-succulent species distributed in hot and dry regions, and is rich in flavonoids, which are considered to be protective against UV-B radiation. This study aims to verify if K. pinnata has morphological or anatomical responses as a strategy in response to high UV-B levels. Kalanchoe pinnata plants of the same age were grown under white light (control) or white light plus supplemental UV-B radiation (5 h d(-1)). The plants were treated with the same photoperiod, photosynthetically active radiation, temperature and daily watering system. Fragments of the middle third of the leaf blade and petiole were dehydrated and then embedded in historesin and sectioned in a rotary microtome. Sections were stained with toluidine blue O and mounted in Entellan®. Microchemical analyses by optical microscopy were performed on fresh material with Sudan III, Sudan IV and phloroglucinol, and analysed using fluorescence microscopy. Supplemental UV-B radiation caused leaf curling and the formation of brown areas on the leaves. These brown areas developed into a protective tissue on the adaxial side of the leaf, but only in directly exposed regions. Anatomically, this protective tissue was similar to a wound-periderm, with outer layer cell walls impregnated with suberin and lignin. This is the first report of wound-periderm formation in leaves in response to UV-B radiation. This protective tissue could be important for the survival of the species in desert regions under high UV-B stress conditions. © The Author 2015. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  12. Effect of benzimidazol-derivatives on the DNA-protein binding formation after UV-radiation of chromatin

    International Nuclear Information System (INIS)

    Mil', E.M.; Binyukov, V.I.; Zhil'tsova, V.M.; Stolyarova, L.G.; Kuznetsov, Yu.V.

    1991-01-01

    Effect of benzimidazol-derivatives on the DNA-protein binding formation was studied after UV-radiation of chromatin. These derivatives were shown to protect chromatin from UV-induced DNA-protein binding formation. Structural analog contained two aminomethyl residuals sensibilized additional binding formation in chromatin. Results suggested, that benzimidazol interacted with DNA, while aminomethyl groups interacted with protein and sensibilized binding of DNA, whilt aminomethyl groups interacted with protein and sensibilized binding of DNA with histone H1

  13. R-B measurements and their implications : solar UV radiation trends

    International Nuclear Information System (INIS)

    Basher, R.

    1993-01-01

    There is a wide variety of needs for UV information, including: (i) real-time monitoring and other public information; (ii) trend detection and policy-maker needs; (iii) space/time/wavelength information for health and biological impacts research; and (iv) atmospheric research, e.g. effects of clouds on UV, transmission model development, etc. The way each need is satisfied will vary. High quality spectral data for research purposes requires expensive equipment and close, on-site scientific supervision and hence will be restricted to a few locations, while conversely, measurements of spatial patterns will require numerous simple instruments, such a the Robertson-Berger Meter or International Light instrument, or the use of satellite methods. Trend detection will lay great store on long-term stability of instrumentation. It is important to recognise that to deal with the variety of needs, we need the combined use of spectral measurement, broadband measurement, radiation modelling, and satellite techniques. (author). 13 refs., 3 figs

  14. Generation and characterization of antigenic variants induced by exposure of tumor cells to UV radiation in vitro

    International Nuclear Information System (INIS)

    Hostetler, L.L.W.

    1988-01-01

    Antigenic changes present in nonantigenic tumor cells exposed to UV radiation (UV) in vitro were investigated by addressing the following questions: (1) Are antigenic variants (AV) produced that are rejected in normal but not immunosuppressed mice? (2) Does generation of AV depend upon intrinsic properties of the cells exposed or result from the action of UV? (3) Is antigenic modification induced by UV due to increased histocompatibility antigen expression? (4) Do AV crossreact immunologically with parental tumor or with other AV? and (5) Is the UV-associated common antigen expressed on UV-induced tumors present on UV-irradiated tumor cells? AV were generated at different frequencies following in vitro UV irradiation of a spontaneous murine fibrosarcoma, a murine melanoma, and two melanoma clones. Immunological experiments demonstrated that the AV and parental cells shared a determinant that was susceptible to immune recognition, but incapable of inducing immunity. In contrast, the AV were noncrossreactive, suggesting that variant-specific antigens were also expressed. Finally, the AV were recognized by UV-induced suppressor cells, indicating that the UV-associated common antigen expressed by UV-induced tumors was also present

  15. Leaf movement, photosynthesis and resource use efficiency responses to multiple environmental stress in Glycine max (soybean)

    International Nuclear Information System (INIS)

    Rosa, L.M.G.

    1993-01-01

    Increases in the concentration of greenhouse gases in the atmosphere, may cause a significant increase in temperature, with implications for general wind patterns and precipitation. Reductions in stratospheric ozone will result in increased levels of UV-B reaching earth's surface. During their lifetime plants must deal with a variety of co-occurring environmental stresses. Accordingly, studies into plant responses to multiple environmental factors is important to our understanding of limits to their growth, productivity, and distribution. Heliotropic leaf movements are a generalized plant response to environmental stresses, and the pattern of these movements can be altered by resource availability (e.g., water, and nitrogen). Previous greenhouse and field studies have demonstrated damaging effects of UV-B radiation in crop species, including soybean. Documented in this paper are Leaf movement and gas exchange responses of four soybean cultivars with different sensitivity to UV-B radiation to enhanced levels of UV-B, and modifications of these responses caused by water stress and nitrogen fertilization. UV-B radiation had no effect on the patterns of leaf orientation in soybean; however, a ranking of the cultivars based on midday leaf angles was the same as the ranking of these cultivars based on their sensitivity to UV-B radiation. Water and nitrogen altered the leaf movement patterns of soybeans. Gas exchange parameters in all four cultivars responded in a similar fashion to changes in leaf water potential. Reductions in water availability resulted in lower discrimination. Nitrogen fertilization in cv Forrest, also resulted in lower discrimination, especially under low water regimes, indicating a higher water use efficiency for fertilized plants. UV-B radiation resulted in lower discrimination in the UV-B sensitive CNS cultivar, indicating a stronger stomatal limitation to photosynthesis under increased UV-B levels

  16. Photosynthetic carbon reduction by seagrasses exposed to ultraviolet A radiation

    Science.gov (United States)

    1979-01-01

    The seagrasses Halophila engelmannii, Halodule wrightii, and Syringodium filiforme were examined for their intrinsic sensitivity to ultraviolet-A-UV-A and ultraviolet-B-UV-B radiation. The effect of UV-A on photosynthetically active radiation (PAR) was also determined. Ultraviolet-A and ultraviolet-B were studied with emphasis on the greater respective environmental consequence in terms of seagrass distribution and abundance. Results indicate that an intrinsic sensitivity to UV-A alone is apparent only in Halophila, while net photosynthesis in Halodule and Syringodium seems unaffected by the level of UV-A provided. The sensitivity of Halophila to UV-A in the absense of (PAR) indicates that the photosynthetic reaction does not need to be in operation for damage to occur. Other significant results are reported.

  17. Studies on antioxidant properties before and after UV- and γ-IR radiation of Bulgarian lavender essential oil isolated from Lavandula Angostifolia Mill

    International Nuclear Information System (INIS)

    Karamalakova, Yanka; Nikolova, Galina; Gadjeva, Veselina; Zheleva, Antoaneta; Sharma, Jyoti; Stanev, Stanko; Arora, Rajesh

    2013-01-01

    At present there is not enough data about different types of radiation on Bulgarian essential oils isolated from medicinal plants. Here we report for the first time our studies on the effects of UV-radiation (290 nm to 320 nm) and γ-radiation (doses of 5 Gy, 10 Gy, 20 Gy and 30 Gy) on Bulgarian lavender essential oil isolated from Lavandulla angustifolia Mill. A spectrophotometry method was used for evaluation of the changes in the reducing power of the Bulgarian lavender oil before and after radiation. The electron donation potential of non-irradiated oil was found to be lower (0.268 ± 0.0244) than those of both, UV-irradiated (0.336 ± 0.0121) and γ-irradiated, oil samples (0.427 ± 0.0251 at 5 Gy radiation, 0.341 ± 0.0371 at 10 Gy; 0.328± 0.0173 at 20 Gy). By direct electron paramagnetic resonance (EPR) spectroscopy, single almost symmetrical EPR signals with different values of the g-factor were registered in non-irradiated (g = 2.0047 ± 0.0002), UV-irradiated (g = 2.01050 ± 0.00005) and γ-irradiated oil samples (g = 2.0017 ± 0.0002), respectively. EPR spectroscopy was used for assessment of the radical scavenging capacity of the non-irradiated and UV- and γ-irradiated samples of the Bulgarian lavender oil towards the stable free radical 1,1-diphenyl- 2-picrylhydrazyl (DPPH). Excellent DPPH radical scavenging capacities were found for UV- and 30 Gy-irradiated lavender oil samples. Based on these preliminary results, we consider that, after proper UV- or γ-radiation treatment, Bulgarian lavender oil might find application as a good radioprotector and antioxidant in cosmetics and medicine

  18. Radiation indicator options for environmental policy

    CERN Document Server

    Pruppers, M J M

    2002-01-01

    It has proven impossible to create an indicator capable of showing the state of the art in a single figure so as to determine the progress made in the 'radiation component' of environmental policy from the trends indicated. This is the conclusion following an investigation requested by the Ministry of Housing, Spatial Planning and the Environment in the framework of the environmental theme 'dispersion of radioactive substances and ionising radiation' to develop a radiation indicator comparable to the indicators for proprietary substances and pesticides. The most important reasons for failing to develop this indicator lie in the absence of both suitable policy targets and data for the calculations necessary for the indicator. Substituting policy targets with reference emissions, reference concentrations and reference doses would make it possible to define indicators for radiation protection. Here, environmental pressure indicators are proposed for nuclear installations and the process industry. The environment...

  19. Lipids and pigment-protein complexes of photosynthetic apparatus of Deschampsia antarctica Desv. plants under UV-B radiation

    Directory of Open Access Journals (Sweden)

    Svietlova N. B.

    2012-01-01

    Full Text Available Aim. To investigate structural and functional modifications of major components of photosynthetic membranes of endemic antarctic species D. antarctica under UV-B radiation. Methods. For quantitative determination of photosynthetic membrane components we used Arnon’s method (for chlorophylls and carotenoids; separation of carotenoids was carried out by Merzlyak’s method; polar lipids were isolated by Zill and Harmon method in modification of Yakovenko and Mihno; glycolipids separation and identification we carried out by Yamamoto method; and sulfoquinovosyl diacylglycerol content was determined by Kean method. The separation, disintegration and determination of pigment-protein complexes of chloroplasts were carried out by Anderson method. Authenticity of differences between the mean arithmetic values of indices was set after the Student criterion. Differences were considered as reliable at p 0.05. Results. We determined structural and functional changes in lipids, carotenoids and pigment-protein complexes at the photosyntetic apparatus level in D. antarctica plants under UV-B radiation. Conclusions. Adaptation of D. antarctica plants to UV-B radiation is accompanied by a cascade of physiological and biochemical rearrangements at the level of photosynthetic apparatus, manifested as the changes in pigment, lipid and pigment-protein complexes content

  20. The effect of ultraviolet-B radiation on gene expression and pigment composition in etiolated and green pea leaf tissue: UV-B-induced changes are gene-specific and dependent upon the developmental stage

    International Nuclear Information System (INIS)

    Jordan, B.R.; James, P.E.; Strid, A.; Anthony, R.G.

    1994-01-01

    The effect of ultraviolet-B radiation (UV-B: 280–320nm) on gene expression and pigment composition has been investigated in pea tissue at different stages of development. Pea (Pisum sativum L., cv. Feltham First) seedlings were grown for 17d and then exposed to supplementary UV-B radiation. Chlorophyll a per unit fresh weight decreased by more than 20% compared with control levels after exposure to UV-B radiation for 7d. In contrast, chlorophyll b content remained the same or increased slightly. Leaf protein biosynthesis, as determined by 35 S-methionine incorporation, was rapidly inhibited by UV-B radiation, although the steady-state levels of proteins were either unchanged or only slightly altered. RNA transcripts for the chlorophyll a/b binding protein (cab) were also rapidly reduced to low or even undetectable levels in the expanded third leaf or younger leaf bud tissue after exposure to UV-B radiation. In contrast, cab RNA transcripts were either low or undetectable in etiolated pea tissue, but increased substantially in light and during exposure to UV-B radiation. The cab RNA transcripts were still present at control levels in pea plants after 7d of greening under supplementary UV-B radiation or UV-B alone. The protein composition changed significantly over the 7d of greening, but no differences could be detected between the light treatments. The increase in chlorophyll content was slightly greater during de-etiolation under supplementary UV-B radiation than under control irradiance. Under UV-B radiation alone, chlorophyll was synthesized at a greatly reduced rate. Changes in protective pigments were also determined. Anthocyanins did not change in either etiolated or green tissue exposed to UV-B radiation. However, other flavonoids increased substantially in either tissue during exposure to light and UV-B radiation. The RNA levels for chalcone synthase were measured in green and etiolated tissue exposed to UV-B radiation. The chs RNA transcripts were

  1. Photosynthetic characteristics and mycosporine-like amino acids under UV radiation: a competitive advantage of Mastocarpus stellatus over Chondrus crispus at the Helgoland shoreline?

    Science.gov (United States)

    Bischof, K.; Kräbs, G.; Hanelt, D.; Wiencke, C.

    2000-05-01

    Chondrus crispus and Mastocarpus stellatus both inhabit the intertidal and upper sublittoral zone of Helgoland, but with C. crispus generally taking a lower position. Measurements of chlorophyll fluorescence, activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), and content and composition of UV absorbing mycosporine-like amino acids (MAAs) were conducted in the laboratory, to test whether susceptibility to UV radiation may play a role in the vertical distribution of these two species. Effective and maximal quantum yield of photochemistry as well as maximal electron transport rate (ETRmax) in C. crispus were more strongly affected by UV-B radiation than in M. stellatus. In both species, no negative effects of the respective radiation conditions were found on total activity of RubisCO. Total MAA content in M. stellatus was up to 6-fold higher than in C. crispus and the composition of MAAs in the two species was different. The results indicate that, among others, UV-B sensitivity may be a factor restricting C. crispus to the lower intertidal and upper sublittoral zone, whereas M. stellatus is better adapted to UV radiation and is therefore more competitive in the upper intertidal zone.

  2. Long-term effects of elevated ozone and UV-B radiation on vegetation and methane dynamics in northern peatland ecosystems

    Energy Technology Data Exchange (ETDEWEB)

    Morsky, S.K.

    2012-06-15

    In the stratosphere, ozone (O{sub 3}) forms an effective barrier against high energy ultraviolet radiation (UV), which is harmful to living cells. Despite the stratospheric O{sub 3} layer recovering due to international agreements, seasonal O{sub 3} depletion periods with high UV-B levels, may still occur, especially in the Polar regions. In the troposphere, O{sub 3} is a significant greenhouse gas contributing to global warming and also causing oxidative stress to animal- and plant cells. Global tropospheric O{sub 3} concentration has approximately doubled during the last century and the same trend is expected to continue. Northern peatlands are sinks of atmospheric carbon dioxide (CO{sub 2}) and sources of the powerful greenhouse gas methane (CH{sub 4}). Two multi-year open-field experiments were conducted to study the effects of elevated O{sub 3} concentration and UV-B radiation on peatland vegetation and CH{sub 4} dynamics in Finland. Peatland microcosms were used in the O{sub 3} experiment and the UV-B exposure study was conducted on a natural fen. Elevated O{sub 3} concentration significantly increased leaf cross-sections and the total number of Eriophorum vaginatum leaves towards the end of the experiment, but did not affect relative length growth, stomatal density or volume of aerenchymatous tissue of leaves. Elevated O{sub 3} did not affect relative length growth of Sphagnum papillosum shoots either. Concentrations of chlorophylls or carotenoids in E. vaginatum leaves or in S. papillosum shoots were not changed under elevated O{sub 3}. During the first growing season, elevated O{sub 3} concentration decreased methanol-extractable, UV-absorbing compounds in E. vaginatum leaves. Elevated O{sub 3} increased concentrations of organic acids and microbial biomass (estimated by phospholipid fatty acid biomarkers) in peat during the third growing season. In the first growing season net CH{sub 4} emission was temporarily decreased by elevated O{sub 3} concentration

  3. Characterization of DNA-repair potential in deep subsurface bacteria challenged by UV light, hydrogen peroxide, and gamma radiation

    OpenAIRE

    Arrage, Andrew Anthony

    1991-01-01

    Subsurface bacterial isolates obtained through the DOE Subsurface Science Program were tested for resistance to UV light, gamma radiation and H202. Some deep subsurface bacteria were resistant to UV light, demonstrating â ¥1.0% survival at fluences which resulted in a 0.0001% survival level of E. coli B. The percentage of UV resistant aerobic subsurface bacteria and surface soil bacteria were similar; 30.8% and 25.8% respectively. All of the microaerophilic subsurface isolates ...

  4. UV/PAR radiations and DOM properties in surface coastal waters of the Canadian shelf of the Beaufort Sea during summer 2009

    Science.gov (United States)

    Para, J.; Charrière, B.; Matsuoka, A.; Miller, W. L.; Rontani, J. F.; Sempéré, R.

    2012-11-01

    Water masses from the Beaufort Sea in the Arctic Ocean were evaluated for dissolved organic carbon (DOC), and optical characteristics including UV and PAR diffuse attenuation (Kd), and chromophoric and fluorescent dissolved organic matter (CDOM and FDOM) as part of the MALINA field campaign (30 July to 27 August). Even with relatively low mean daily solar radiation incident on the sea surface (0.12 ± 0.03, 8.46 ± 1.64 and 18.09 ± 4.20 kJ m-2 for UV-B (305 nm), UV-A (380 nm) and PAR, respectively), we report significant light penetration with 10% irradiance depths (Z10% (λ)) reaching 9.5 m for 340 nm (UV-A) radiation in the Eastern sector and 4.5 m in the Mackenzie River influenced area (Western sector). Spectral absorption coefficients (aCDOM (350 nm) (m-1)) were significantly correlated to both diffuse attenuation coefficients (Kd) in the UV-A and UV-B and to DOC concentrations. This indicates CDOM as the dominant attenuator of UV solar radiation and suggests its use as an optical proxy for DOC concentrations in this region. Extrapolating CDOM to DOC relationships, we estimate that ~ 16% of the DOC in the Mackenzie River does not absorb radiation at 350 nm. DOC and CDOM discharges by the Mackenzie River during the MALINA Cruise are estimated as ~ 0.22 TgC and 0.18 TgC, respectively. Three dissolved fluorescent components (C1-C3) were identified by fluorescence Excitation/Emission Matrix Spectroscopy (EEMS) and PARAFAC analysis. Our results showed an in-situ biological component (C1) that co-dominated with a terrestrial humic-like component (C2) in the Mackenzie Delta sector, whereas the protein-like (C3) component dominated in the saltiest waters of the North East sector.

  5. Effect of UV-B and high visual radiation on photosynthesis in freshwater (nostoc spongiaeforme) and marine (Phormidium corium) cyanobacteria.

    Science.gov (United States)

    Bhandari, Rupali; Sharma, Prabhat Kumar

    2007-08-01

    Human activity is causing depletion of ozone in stratosphere, resulting in increased UV-B radiation and global warming. However, impact of these climatic changes on the aquatic organism (especially marine) is not fully understood. Here, we have studied the effect of excess UV-B and visible radiation on photosynthetic pigments, fatty acids content, lipid peroxidation, nitrogen content, nitrogen reductase activity and membrane proteins, induction of mycosporine-like amino acids (MAAs) and antioxidant enzymes superoxide dismutase (SOD) and ascorbate peroxidase (APX) in freshwater (Nostoc spongiaeform) and marine (Phormidium corium) cyanobacteria. UV-B treatment resulted in an increase in photosynthetic pigments in Nostoc and decrease in Phormidium, but high light treatment caused photobleaching of most of the pigments in both the species. Unsaturation level of fatty acids of both total and glycolipids remained unchanged in both the cyanobacteria, as a result of UV-B and high light treatments. Saturated fatty acids of total and glycolipids declined slightly in Nostoc by both the treatments. but remained unchanged in Phormidium. No changes in the unsaturated lipid content in our study probably suggested adaptation of the organism to the treatments. However, both treatments resulted in peroxidation of membrane lipids, indicating oxidative damage to lipids without any change in the level of unsaturation of fatty acid in the cell membrane. Qualitative and quantitative changes were observed in membrane protein profile due to the treatments. Cyanobacteria were able to synthesize MAAs in response to the UV-B treatment. Both treatments also increased the activities of SOD and APX. In conclusion, the study demonstrated induction of antioxidants such as SOD and APX under visible light treatment and screening pigment (MAAs) under UV-B treatment, which might protect the cyanobacteria from oxidative damage caused by high light and UV-B radiation.

  6. Simultaneous demonstration of UV-type and ionizing radiation-type DNA repair by the nucleoid sedimentation technique

    International Nuclear Information System (INIS)

    Aldenhoff, P.; Sperling, K.

    1984-01-01

    The nucleoid sedimentation technique is one of the most sensitive methods for measuring DNA excision repair. With this technique, it is shown that both UV- and ionizing radiation-type repair (the latter induced by bleomycin) can be discriminated in HeLa and normal diploid cells using 1-β-D-arabinofuranosylcytosine. The latter compound inhibits UV-type repair synthesis, and thus causes DNA breaks due to enzymic incision to persist, but has no effect on rejoining DNA after ionizing radiation-type damage. It was then possible to prove that 4-nitroquinoline-1-oxide induces both types of lesions which are repaired simultaneously. This effect could be demonstrated in HeLa and normal human diploid cells in a single experimental set-up. (Auth.)

  7. Operational surface UV radiation product from GOME-2 and AVHRR/3 data

    OpenAIRE

    J. Kujanpää; N. Kalakoski

    2015-01-01

    The surface ultraviolet (UV) radiation product, version 1.20, generated operationally in the framework of the Satellite Application Facility on Ozone and Atmospheric Chemistry Monitoring (O3M SAF) of the European Organisation for the Exploitation of Meteorological Satellites (EUMETSAT) is described. The product is based on the total ozone column derived from the measurements of the second Global Ozone Monitoring Experiment (GOME-2) instrument aboard EUMETSA...

  8. Implications of mycosporine-like amino acid and antioxidant defenses in UV-B radiation tolerance for the algae species Ptercladiella capillacea and Gelidium amansii.

    Science.gov (United States)

    Lee, Tse-Min; Shiu, Chia-Tai

    2009-02-01

    Ultraviolet-B (UV-B) radiation (0.5, 1.0, 1.5, and 3.0Wm(-2)) induced higher H(2)O(2) production and lipid peroxidation in alga Gelidium amansii inhabiting in lower subtidal regions than upper subtidal alga Ptercladiella capillacea. Compared to G. amansii, mycosporine-like amino acid (MAA) concentration in P. capillacea was higher and can be increased by 0.5-1.0Wm(-2) UV-B, while carotenoid concentration was lower but also increased by 1.5-3.0Wm(-2) UV-B. UV-B increased ascorbate concentration, but to a higher degree in P. capillacea. UV-B decreased glutathione concentration, but to a higher degree in G. amansii. UV-B increased ascorbate peroxidase (APX) and glutathione reductase (GR) activities in P.capillacea but decreased them in G. amansii. UV-B increased superoxide dismutase and catalase activities, but to a higher degree in G. amansii. So, G. amansii suffered greater oxidative stress from UV-B radiation. P. capillacea can effectively reduce UV-B sensitivity by increasing sunscreen ability and antioxidant defense capacity.

  9. The sunburn cell in hairless mouse epidermis: quantitative studies with UV-A radiation and mono- and bifunctional psoralens

    International Nuclear Information System (INIS)

    Young, A.R.; Magnus, I.A.

    1982-01-01

    The production of the sunburn cell by UV-A radiation and topical psoralens in hairless mouse epidermis has been studied. It has been shown that the appearance of this cell is dependent on the dose of both UV-A radiation and of the psoralen. The time-course with 8-methoxypsoralen has peak sunburn cell numbers at 28 hr postirradiation. A comparison of 2 bifunctional (8-methoxypsoralen and 5-methoxypsoralen) and 2 monofunctional (angelicin and 3-carbethoxypsoralen) psoralens showed the former are more potent. This suggests that DNA crosslink lesions may play a rle in sunburn cell production

  10. Crosstalk between MAV and MEP pathways in vitro grape plants exposed to UV-B radiation

    International Nuclear Information System (INIS)

    Gil, M.; Bottini, R.; Piccoli, P.; Pontin, M.

    2010-01-01

    The synthesis of terpenoids from IPP (isopentenyl diphosphate) proceeds in plants throughout two pathways, the MVA (mevalonic acid) in cytosol and the MEP (2-C-methyl-D-erythritol 4-phosphate) in plastids. Ultraviolet-B (UV-B) radiation induced the synthesis of terpenes in in vitro grape plants according to the fluence rate. Low intensity UV-B promoted the MVA pathway while high intensity UV-B stimulated the MEP pathway. Mevastatin is known to inhibit the enzyme HMG-CoA reductase blocking terpene synthesis in cytosol. In vitro plants growing 45 days under 16 h-photoperiod (100 μmol m - 2 s - 1) were fed at the apex with mevastatin and then exposed to an UV-B dose administrated at two intensities: low UV-B (8.25 μW cm - 2,16 h) or high UV-B (33 μW cm - 2,4 h). Methanol: chloroform extracts were analyzed by GC-EIMS and compared with controls without mevastatin. Levels of γ-Sitosterol and Stigmasterol were significantly increased under low intensity UV-B in the controls. The plants treated with the inhibitor showed a significant decrease of both sterols and a decrease in the plastidial terpenes but sterols were higher under UV-B. These results suggest an IPP crosstalk between the MAV and MEP pathways under restrictive conditions. (authors)

  11. In vitro recombination of bacteriophage T7 DNA damaged by uv radiation

    International Nuclear Information System (INIS)

    Masker, W.E.; Kuemmerle, N.B.

    1980-01-01

    A system capable of in vitro packaging of exogenous bacteriophage T7 DNA has been used to monitor the biological activity of DNA replicated in vitro. This system has been used to follow the effects of uv radiation on in vitro replication and recombination. During the in vitro replication process, a considerable exchange of genetic information occurs between T7 DNA molecules present in the reaction mixture. This in vitro recombination is reflected in the genotype of the T7 phage produced after in vitro encapsulation; depending on the genetic markers selected, recombinants can comprise nearly 20% of the total phage production. When uv-irradiated DNA is incubated in this system, the amount of in vitro synthesis is reduced and the total amount of viable phage produced after in vitro packaging is diminished. In vitro recombination rates are also lower when the participating DNA molecules have been exposed to uv. However, biochemical and genetic measurements confirmed that there is little or no transfer of pyrimidine dimers from irradiated DNA into undamaged molecules

  12. Effect of UV-C radiation and hypergravity on germination, growth and content of chlorophyll of wheat seedlings

    Energy Technology Data Exchange (ETDEWEB)

    Rupiasih, N. Nyoman, E-mail: rupiasih@gmail.com [Department of Physics, Udayana University, Bali (Indonesia); Vidyasagar, Pandit B., E-mail: prof-pbv@yahoo.com [Biophysics Laboratory, Department of Physics, University of Pune, Pune-411007 (India)

    2016-03-11

    An investigation of the effects of UV-C radiation and hypergravity on germination, growth and content of chlorophyll of wheat seedlings has been done. The UV-C irradiation periods of exposure were 30, 60, 90, 120 and 180 minutes. The hypergravity used were 1000 g, 2000 g and 2500 g. The combination treatment is UV-C irradiation for 180 min followed by each hypergravity. The results showed that irradiation of UV-C on wheat seeds have stimulated the seed germination, but hypergravity and combination treatments on wheat seeds have inhibited the seed germination. Those treatments gave negative effects to growth rate, the content of chlorophyll a, b and total chlorophyll of wheat seedlings.

  13. Effect of UV-C radiation and hypergravity on germination, growth and content of chlorophyll of wheat seedlings

    International Nuclear Information System (INIS)

    Rupiasih, N. Nyoman; Vidyasagar, Pandit B.

    2016-01-01

    An investigation of the effects of UV-C radiation and hypergravity on germination, growth and content of chlorophyll of wheat seedlings has been done. The UV-C irradiation periods of exposure were 30, 60, 90, 120 and 180 minutes. The hypergravity used were 1000 g, 2000 g and 2500 g. The combination treatment is UV-C irradiation for 180 min followed by each hypergravity. The results showed that irradiation of UV-C on wheat seeds have stimulated the seed germination, but hypergravity and combination treatments on wheat seeds have inhibited the seed germination. Those treatments gave negative effects to growth rate, the content of chlorophyll a, b and total chlorophyll of wheat seedlings.

  14. Induced resistance to hydrogen peroxide, UV and gamma radiation in bacillus species

    International Nuclear Information System (INIS)

    Bashandy, A.S.

    2005-01-01

    The catalase activity produced in four bacillus spp.(bacillus cereus, B. laterosporus, B. pumilus and B. subtilis (Escherichia coli was used for comparison) was measured and the sensitivity of these bacteria to hydrogen peroxide was tested. Bacillus spp. had higher resistance to hydrogen peroxide than E. coil. cultures of bacillus spp . When pretreated with sublethal level of hydrogen peroxide, became relatively resistant to the lethal effects of hydrogen than untreated control cultures. These pretreated cells were also resistant to lethality mediated by UV light and gamma radiation. The obtained results suggest that bacillus spp. Possess inducible defense mechanism (s) against the deleterious effects of oxidants and /or ionizing radiation

  15. UV Tanning Equipment | Radiation Protection | US EPA

    Science.gov (United States)

    2017-08-07

    Sun lamps and tanning equipment emit ultraviolet (UV) rays. People who are exposed to UV rays over a long period of time are more likely to develop skin cancer. People with light skin are in more danger because their skin is more sensitive to UV rays.

  16. Non-photosynthetic mechanisms of growth reduction in pea (Pisum sativum L.) exposed to UV-B radiation

    International Nuclear Information System (INIS)

    Gonzalez, R.; Mepsted, R.; Wellburn, A.R.; Paul, N.D.

    1998-01-01

    Pisum sativum cv. Guido grown under controlled environment conditions was exposed to either low or high UV-B radiation (2·2 or 9·9 kJ m –2 d –1 plant-weighted UV-B, respectively). Low or high UV-B was maintained throughout growth (LL and HH treatments, respectively) or plants were transferred between treatments when 22 d old (giving LH and HL treatments). High UV-B significantly reduced plant dry weight and significantly altered plant morphology. The growth and morphology of plants transferred from low to high UV-B were little affected, when compared with those of LL plants. By contrast, plants moved from high to low UV-B showed marked increases in growth when compared with HH plants. This contrast between HL and LH appeared to be related to the effect of UV-B on plant development. Exposure to high UV-B throughout development consistently reduced leaf areas. In fully expanded leaves there was no significant UV-B effect on cell area and reduced leaf area could be attributed to reduced cell number, suggesting effects on leaf primordia. Further reductions in the leaf area of younger leaves were the result of the slower development rate of plants grown at high UV-B, which also resulted in significant reductions in leaf number. (author)

  17. Lanthanum (III) regulates the nitrogen assimilation in soybean seedlings under ultraviolet-B radiation.

    Science.gov (United States)

    Huang, Guangrong; Wang, Lihong; Zhou, Qing

    2013-01-01

    Ultraviolet-B (UV-B, 280-320 nm) radiation has seriously affected the growth of plants. Finding the technology/method to alleviate the damage of UV-B radiation has become a frontal topic in the field of environmental science. The pretreatment with rare earth elements (REEs) is an effective method, but the regulation mechanism of REEs is unknown. Here, the regulation effects of lanthanum (La(III)) on nitrogen assimilation in soybean seedlings (Glycine max L.) under ultraviolet-B radiation were investigated to elucidate the regulation mechanism of REEs on plants under UV-B radiation. UV-B radiation led to the inhibition in the activities of the key enzymes (nitrate reductase, glutamine synthetase, glutamate synthase) in the nitrogen assimilation, the decrease in the contents of nitrate and soluble proteins, as well as the increase in the content of amino acid in soybean seedlings. The change degree of UV-B radiation at the high level (0.45 W m(-2)) was higher than that of UV-B radiation at the low level (0.15 W m(-2)). The pretreatment with 20 mg L(-1) La(III) could alleviate the effects of UV-B radiation on the activities of nitrate reductase, glutamine synthetase, glutamate synthase, and glutamate dehydrogenase, promoting amino acid conversion and protein synthesis in soybean seedlings. The regulation effect of La(III) under UV-B radiation at the low level was better than that of UV-B radiation at the high level. The results indicated that the pretreatment with 20 mg L(-1) La(III) could alleviate the inhibition of UV-B radiation on nitrogen assimilation in soybean seedlings.

  18. Effect of ZnO nano in the blend PET / PC ( 80/20) subjected to UV radiation

    International Nuclear Information System (INIS)

    Pires, Homero M.; Mendes, Luis C.; Albitres, Gerson A.V.; Cestari, Sibele P.; Mattos, Gabriela C.

    2015-01-01

    The aim of this research was to reuse recycled poly(ethylene terephthalate) (PET) in blend with polycarbonate (PC) in order to avoid UV degradation in outdoor application. Nanocomposite based on blend of recycled (PET) and polycarbonate (PC) in the ratio of 80/20 with nano zinc oxide (ZnO) at different concentrations was prepared. The blend was subjected to ultraviolet (UV) radiation for accelerated aging chamber, we evaluated the effect of the presence nZnO filler as a barrier to UV rays. Dynamic-mechanical analysis (DMA) and nuclear magnetic resonance in the solid state (NMR). It is the degradation retardant effect at concentrations starting from 3% nZnO. (author)

  19. Suggestions for the Improvement of Environmental Radiation Monitoring in Kenya

    International Nuclear Information System (INIS)

    Shadrack, A. K.

    2012-01-01

    Environmental radiation monitoring in Kenya was started in 1990 following the 1979 Three Mile Island and the 1986 Chernobyl nuclear power plants accidents. The main purpose was to measure the radioactivity of foodstuffs imported from oversees and to carry out environmental radiation monitoring of soil, rock, water and air sample to check for contamination. Through environmental radiation monitoring, the Food and Environmental Monitoring Section (FEM) of the Kenya Radiation Protection Board (RPB) works to protect the public and environment from hazards associated with ionizing radiation. The purpose of this paper was to highlight suggestions for the improvement of environmental radiation monitoring in Kenya with respect to protecting the public and the environment against undue radiation risk by ensuring that potential exposures are kept As Low As Reasonably Achievable (ALARA). The suggestions for improvement will serve as a guideline for the strengthening of environmental radiation monitoring program in Kenya

  20. Influence of photosynthetically active radiation and spectral quality on UV-B-induced polyamine accumulation in soybean

    International Nuclear Information System (INIS)

    Kramer, G.F.; Krizek, D.T.; Mirecki, R.M.

    1992-01-01

    UV-B-sensitive (Essex) and -insensitive (Williams) cultivars of soybean (Glycine max) were grown in growth chambers at photosynthetically active radiation (PAR) levels of 300 or 600 μmol m −2 sec −1 provided by either red- and far-red-deficient (MH) or blue-deficient (HPS/DX) lamps or a combination of both. The combined treatment provided a balanced output, similar to that provided by fluorescent plus incandescent lighting across the visible spectrum. Under the combined lamps, plants were exposed to 12 kJ m −2 day −1 of biologically effective UV-B (UV-B BE ) with 6-hr irradiance periods centred midway through the photoperiod. This irradiance corresponded to a decrease in stratospheric ozone of ca 20% for clear sky conditions at Beltsville, MD on 21 June. Plant growth was significantly inhibited by UV-B at 300 but not at 600 μmol m −2 sec −1 PAR. No cultivar differences were noted in the UV-B-induced inhibition of growth, although visible injury was less in Williams than in Essex. PAR had a large effect on polyamine levels in leaves, with higher levels of putrescine (Put) and spermidine observed at 600 than at 300 μmol m −2 sec −1 in both cultivars. UV-B-induced polyamine accumulation was observed primarily in Williams. Under MH or HPS/DX lamps alone, plants were exposed to two different UV-B levels, 9.9 and 12 kJ m −2 day −1 , corresponding to stratospheric ozone reductions of ca 9 and 20%. UV-B inhibited growth at both 300 and 600 μmol m −2 sec −1 PAR under either radiation source. There was no effect of PAR on the UV-B-induced growth inhibition with the HPS/DX lamps, but a partial amelioration of this inhibition occurred in Williams at 600 μmol m −2 sec −1 PAR under MH lamps. Dose-dependent UV-B-induced polyamine accumulation was also observed in both cultivars. PAR increased Put levels under MH but not HPS/DX lamps. These results indicate that the inhibition of UV-B stress by high PAR may require a balance of red and blue

  1. Arctic, Antarctic, and temperate green algae Zygnema spp. under UV-B stress: vegetative cells perform better than pre-akinetes.

    Science.gov (United States)

    Holzinger, Andreas; Albert, Andreas; Aigner, Siegfried; Uhl, Jenny; Schmitt-Kopplin, Philippe; Trumhová, Kateřina; Pichrtová, Martina

    2018-02-22

    Species of Zygnema form macroscopically visible mats in polar and temperate terrestrial habitats, where they are exposed to environmental stresses. Three previously characterized isolates (Arctic Zygnema sp. B, Antarctic Zygnema sp. C, and temperate Zygnema sp. S) were tested for their tolerance to experimental UV radiation. Samples of young vegetative cells (1 month old) and pre-akinetes (6 months old) were exposed to photosynthetically active radiation (PAR, 400-700 nm, 400 μmol photons m -2  s -1 ) in combination with experimental UV-A (315-400 nm, 5.7 W m -2 , no UV-B), designated as PA, or UV-A (10.1 W m -2 ) + UV-B (280-315 nm, 1.0 W m -2 ), designated as PAB. The experimental period lasted for 74 h; the radiation period was 16 h PAR/UV-A per day, or with additional UV-B for 14 h per day. The effective quantum yield, generally lower in pre-akinetes, was mostly reduced during the UV treatment, and recovery was significantly higher in young vegetative cells vs. pre-akinetes during the experiment. Analysis of the deepoxidation state of the xanthophyll-cycle pigments revealed a statistically significant (p UV-absorbing phenolic compounds was significantly higher (p UV-B stress than pre-akinetes.

  2. Wavelength dependence of biological damage induced by UV radiation on bacteria.

    Science.gov (United States)

    Santos, Ana L; Oliveira, Vanessa; Baptista, Inês; Henriques, Isabel; Gomes, Newton C M; Almeida, Adelaide; Correia, António; Cunha, Ângela

    2013-01-01

    The biological effects of UV radiation of different wavelengths (UVA, UVB and UVC) were assessed in nine bacterial isolates displaying different UV sensitivities. Biological effects (survival and activity) and molecular markers of oxidative stress [DNA strand breakage (DSB), generation of reactive oxygen species (ROS), oxidative damage to proteins and lipids, and the activity of antioxidant enzymes catalase and superoxide dismutase] were quantified and statistically analyzed in order to identify the major determinants of cell inactivation under the different spectral regions. Survival and activity followed a clear wavelength dependence, being highest under UVA and lowest under UVC. The generation of ROS, as well as protein and lipid oxidation, followed the same pattern. DNA damage (DSB) showed the inverse trend. Multiple stepwise regression analysis revealed that survival under UVA, UVB and UVC wavelengths was best explained by DSB, oxidative damage to lipids, and intracellular ROS levels, respectively.

  3. Relationship Between Landscape Character, UV Exposure, and Amphibian Decline

    Science.gov (United States)

    O'Reilly, C. M.; Brooks, P. D.; Corn, P. S.; Muths, E.; Campbell, D. H.; Diamond, S.; Tonnessen, K.

    2001-12-01

    Widespread reports of amphibian declines have been considered a warning of large-scale environmental degradation, yet the reasons for these declines remain unclear. This study suggests that exposure to ultraviolet radiation may act as an environmental stressor that affects population breeding success or susceptibility to disease. Ultraviolet radiation is attenuated by dissolved and particulate compounds in water, which may be of either terrestrial or aquatic origin. UV attenuation by dissolved organic carbon (DOC) is primarily due to compounds in the fulvic acid fraction, which originate in soil environments. These terrestrially-derived fulvic acids are transported to during hydrologic flushing events such as snowmelt and episodic precipitation and play an important role in controlling UV exposure in surface waters. As part of a previously published project, amphibian surveys were conducted at seventeen sites in Rocky Mountain National Park both during, and subsequent to, a three-year drought (1988 - 1990). During this period, ten sites lost one amphibian species, while only one site gained a previously unreported species. One possible explanation for these localized species losses is increased exposure to UV radiation, mediated by reduced terrestrial DOC inputs during dry periods. Several subsequent years of water chemistry data showed that the sites with documented species losses were characterized by a range of DOC concentrations, but tended to have a greater proportion of terrestrial DOC than sites that did not undergo species loss. This suggests that terrestrial inputs exert a strong control on DOC concentrations that may influence species success. We used physical environmental factors to develop a classification scheme for these sites. There are many physical factors that can influence terrestrial DOC inputs, including landscape position, geomorphology, soil type, and watershed vegetation. In addition, we considered the possible effects on internal aquatic

  4. Impact of UV-B (290-320 nm) radiation on photosynthesis-mediated uptake of 15N-ammonia and 15N-nitrate of several marine diatoms

    International Nuclear Information System (INIS)

    Doehler, G.; Stolter, H.

    1986-01-01

    The marine diatoms Ditylum brigthwellii, Lithodesmium variabile, Odontella sinensis, Synedra planctonica and Thalassiosira rotula grown at 18 0 C under normal air conditions (0.035 vol.% CO 2 ) were exposed to different levels (439 and 717 J m -2 d -1 , weighted) of UV-B radiation for 2 d (5 h/d). Pigmentation, protein and total nitrogen content were reduced linearly to the dose of UV-B radiation. Photosynthesis-mediated uptake of 15 N-ammonia was more affected by UV-B irradiance in all tested diatoms than that of 15 N-nitrate. A species-dependent behavior in the assimilation of inorganic nitrogenous compounds has been observed: Synedra was a very sensitive species to UV-B radiation whereas the same UV-B doses had no effect on the assimilation rate of ammonia and nitrate of the Lithodesmium cells. The results were discussed with reference to the inhibition of the enzymes of the nitrogen metabolism. (author)

  5. Penetration of UV-B radiation in foliage: evidence that the epidermis behaves as a non-uniform filter

    International Nuclear Information System (INIS)

    Day, T.A.; Martin, G.; Vogelmann, T.C.

    1993-01-01

    In some plants, particularly herbaceous species, a considerable proportion of incident ultraviolet-B radiation (UV-B, 280-320 nm) penetrates into the leaf mesophyll where it is potentially damaging to nucleic acids and the photosynthetic machinery. We used optical techniques to look at the spatial variation in UV-B penetration through the epidermis of foliage of two herbaceous species (Chenopodium album and Smilacina stellata) and a conifer (Picea pungens). Measurements of UV-B penetration in intact foliage with a fibre-optic microprobe revealed that 300 nm radiation reached 161±36μm (mean±SD) into leaves of C. album, 154±40μm in S. stellata and 17±2μm in P. pungens, with epidermal transmittance being 39±14%, 55±19% and 0%, respectively. A thin polymer film was developed which fluoresced blue when irradiated by UV-B. Fresh epidermal leaf peels were placed over the film and irradiated with UV-B, and microscopic examination of the film from below allowed us to determine the spatial pattern of UV-B penetration through the epidermis. In herbaceous species, film fluorescence below cell walls, but not epidermal and guard cell protoplasts indicated that UV-B transmittance was much greater through anticlinal cell wall regions than protoplasts. Ultraviolet-B transmittance through large areas of epidermal cells could be induced by plasmolysis. Epidermal transmittance was also relatively high through stomal pores (and what appear to be nuclei in Smilacina), but relatively low through stomatal guard cells. Results from the fluorescing film technique were substantiated by direct measurements of UV-B transmittance through epidermal peels with a fibre-optic microprobe run paradermally along the bottom or inner side of irradiated peels. In Smilacina, we estimate that UV-B epidermal transmittance was up to 90% through anticlinal cell wall regions, but <10% through protoplast areas. In contrast to herbaceous species, we did not detect any UV-B transmittance through the

  6. Study of mechanical behavior of PMMA in bending and after UV irradiation and gamma radiation; Estudo do comportamento mecanico do PMMA sob flexao apos irradiacao UV e gama

    Energy Technology Data Exchange (ETDEWEB)

    Todt, M.L.; Kienen, V.D.; Azevedo, E.C., E-mail: helunica@yahoo.com.br [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil)

    2014-07-01

    PMMA is a polymer that has density similar to water and refractive index alike to glass. It has been used in the substitution to roofing tiles and coverages, affording to be exposed to UV radiation and gamma radiation. This paper had the objective to study the effect in the flexural proprieties of the PMMA exposed to these types of radiations and the evaluation of the wettability through a contact angle measurer. The PMMA specimens have been submitted to 1500 h of UVA radiation, 1500 h of UVC radiation and to 25kGy of gamma radiation. The results show that the PMMA. (author)

  7. Low-level laser therapy: Effects on human face aged skin and cell viability of HeLa cells exposed to UV radiation

    Directory of Open Access Journals (Sweden)

    Mezghani Sana

    2015-01-01

    Full Text Available Chronic and excessive exposure to UV radiation leads to photoaging and photocarcinogenesis. Adequate protection of the skin against the deleterious effects of UV irradiation is essential. Low-level laser therapy (LLLT is a light source in the red to near-infrared range that has been accepted in a variety of medical applications. In this study, we explored the effect of LLLT in human face aged skin and the cell viability of HeLa cells exposed to UV radiation. We found that LLLT significantly reduced visible wrinkles and the loss of firmness of facial skin in aging subjects. Additionally, treatment of cultured HeLa cells with LLLT prior to or post UVA or UVB exposure significantly protected cells from UV-mediated cell death. All results showed the beneficial effects of LLLT on relieving signs of skin aging and its prevention and protection of the cell viability against UV-induced damage.

  8. Sensitivity of pathogenic and free-living Leptospira spp. to UV radiation and mitomycin C

    International Nuclear Information System (INIS)

    Stamm, L.V.; Charon, N.W.

    1988-01-01

    The habitats for the two major Leptospira spp. differ. The main habitat of L. biflexa is soil and water, whereas L. interrogans primarily resides in the renal tubules of animals. We investigated whether these two species, along with L. illini (species incertae sedis), differ with respect to their sensitivity to UV radiation. The doses of UV resulting in 37, 10 and 1% survival were determined for representive serovars from each species. L. interrogans serovar pomona was 3.0 to 4.8 times more sensitive to UV than the other Leptospira species under the 37, 10, and 1% survival parameters. In comparison to other bacteria, L. interrogans serovar pomona is among the most sensitive to UV. In a qualitative UV sensitivity assay., L. interrogans serovars were found to be in general more sensitive than L. biflexa serovars. All three species were found to have a photoreactivation DNA repair mechanism. Since organisms that are resistant to UV are often resistant to the DNA cross-linking agent mitomycin C, we tested the relative sensitivity of several Leptospira serovars to this compound. With few exceptions, L. biflexa and L. illini serovars were considerably more resistant to mitomycin C than the L. interrogans serovars. The mitomycin C sensitivity assay could be a useful addition to current characterization tests used to differentiate the Leptospira species

  9. Sensitivity of pathogenic and free-living Leptospira spp. to UV radiation and mitomycin C

    Energy Technology Data Exchange (ETDEWEB)

    Stamm, L.V.; Charon, N.W.

    1988-03-01

    The habitats for the two major Leptospira spp. differ. The main habitat of L. biflexa is soil and water, whereas L. interrogans primarily resides in the renal tubules of animals. We investigated whether these two species, along with L. illini (species incertae sedis), differ with respect to their sensitivity to UV radiation. The doses of UV resulting in 37, 10 and 1% survival were determined for representive serovars from each species. L. interrogans serovar pomona was 3.0 to 4.8 times more sensitive to UV than the other Leptospira species under the 37, 10, and 1% survival parameters. In comparison to other bacteria, L. interrogans serovar pomona is among the most sensitive to UV. In a qualitative UV sensitivity assay., L. interrogans serovars were found to be in general more sensitive than L. biflexa serovars. All three species were found to have a photoreactivation DNA repair mechanism. Since organisms that are resistant to UV are often resistant to the DNA cross-linking agent mitomycin C, we tested the relative sensitivity of several Leptospira serovars to this compound. With few exceptions, L. biflexa and L. illini serovars were considerably more resistant to mitomycin C than the L. interrogans serovars. The mitomycin C sensitivity assay could be a useful addition to current characterization tests used to differentiate the Leptospira species.

  10. Regulation of miR-21 expression in human melanoma via UV-ray-induced melanin pigmentation.

    Science.gov (United States)

    Lin, Kuan-Yu; Chen, Chien-Min; Lu, Cheng-You; Cheng, Chun-Yuan; Wu, Yu-Hsin

    2017-08-01

    Excessive environmental ultraviolet (UV) radiation produces genetic mutations that can lead to skin cancer. This study was designed to assess the potential inhibitory activity of microRNA-21 (miR-21) on the UV irradiation-stimulated melanogenesis signal pathway in melanoma cells. The molecular mechanism of miR-21-induced inhibitory activity on UV-ray-stimulated melanogenesis-regulating proteins was examined in A375.S2 human melanoma and B16F10 mouse melanoma cells. UV irradiation for 30 min induced melanogenesis signal pathway by increasing melanin production and the number of A375.S2 cells. Similarly, UV radiation increased the expression of α-melanocyte-stimulating hormone (α-MSH) protein and decreased the melanogenesis-regulating signal, such as EGFR and Akt phosphorylation. Notably, miR-21 overexpression in UV-ray-stimulated A375.S2 cells decreased α-MSH expression and increased EGFR and Akt phosphorylation levels. Furthermore, miR-21 on UV-ray- induced melanogenesis was down-regulated by the Akt inhibitor and the EGFR inhibitor (Gefitinib). Results suggest that the suppressive activity of miR-21 on UV-ray-stimulated melanogenesis may involve the down-regulation of α-MSH and the activation in both of EGFR and Akt. © 2017 Wiley Periodicals, Inc.

  11. Association of UV radiation with Parkinson disease incidence: A nationwide French ecologic study

    International Nuclear Information System (INIS)

    Kravietz, Adam; Kab, Sofiane; Wald, Lucien; Dugravot, Aline; Singh-Manoux, Archana; Moisan, Frédéric; Elbaz, Alexis

    2017-01-01

    Background: Vitamin D is thought to contribute to brain health, but it is unclear whether low vitamin D levels are associated with increased incidence of Parkinson's disease (PD). Using ultraviolet B (UV-B) as a surrogate for vitamin D levels, we conducted a nationwide ecologic study in France in order to examine the association of UV-B with PD incidence. Methods: We used French national drug claims databases to identify PD cases using a validated algorithm. UV-B data from the solar radiation database were derived from satellite images. We estimated PD incidence (2010–2012) at the canton level (small administrative French unit) and used multilevel Poisson regression to examine its association with UV-B (2005 annual average), after adjustment for age, sex, deprivation index, density of neurologists, smoking, proportion of agricultural land, and vitamin D supplementation. Results: Analyses are based on 69,010 incident PD patients. The association between UV-B and PD incidence was quadratic (P<0.001) and modified by age (P<0.001). Below 70y, incidence was higher in the bottom quintile (relative risk, RR Q1:45-49y =1.18, 95% CI=1.08–1.29) compared with the middle UV-B quintile, and lower in the top quintile (RR Q5:45-49y =0.85 [0.77–0.94]). An opposite pattern was observed in older subjects (RR Q1:85-89y =0.92 [0.89–0.96]; RR Q5:85-89y =1.06 [1.02–1.11]). Analysis based on continuous UV-B yielded similar conclusions. Conclusions: In this nationwide study, there was an age-dependent quadratic association between UV-B and PD incidence. This study suggests that reasonable UV-B exposure is associated with lower PD risk in younger persons and that future studies should examine dose-response relations and take age into account. - Highlights: • There is an age-dependent quadratic association between UV-B and PD incidence. • Reasonable UV-B exposure is beneficial in younger persons for the risk of PD. • Our findings are consistent with a role of vitamin D

  12. Intra- and interspecific differences of 10 barley and 10 tomato cultivars in response to short-time UV-B radiation: A study analysing thermoluminescence, fluorescence, gas-exchange and biochemical parameters

    International Nuclear Information System (INIS)

    Gilbert, Matthias; Poers, Yvonne; Grover, Kirsten; Weingart, Ilka; Skotnica, Jiri; Grimm, Bernhard; Seidlitz, Harald K.; Langebartels, Christian; Wilhelm, Christian

    2009-01-01

    The impact of UV-B radiation on 10 genotypically different barley and tomato cultivars was tested in a predictive study to screen for potentially UV-tolerant accessions and to analyze underlying mechanisms for UV-B sensitivity. Plant response was analyzed by measuring thermoluminescence, fluorescence, gas exchange and antioxidant status. Generally, barley cultivars proved to be much more sensitive against UV-B radiation than tomato cultivars. Statistical cluster analysis could resolve two barley groups with distinct differences in reaction patterns. The UV-B sensitive group showed a stronger loss in PSII photochemistry and a lower gas-exchange performance and regulation after UV-B radiation compared to the more tolerant group. The results indicate that photosynthetic light and dark reactions have to play optimally in concert to render plants more tolerant against UV-B radiation. Hence, measuring thermoluminescence/fluorescence and gas exchange in parallel will have much higher potential in identifying tolerant cultivars and will help to understand the underlying mechanisms. - Mechanisms of UV-B tolerance and sensitivity in crop plants.

  13. Alterations in the antibacterial potential of Synechococcus spp. PCC7942 under the influence of UV-B radiations on skin pathogens

    Directory of Open Access Journals (Sweden)

    Nida Fatima

    2017-11-01

    Full Text Available Marine organisms are seen as a source of novel drugs and the discovery of new pharmaceutical is increasingly in demand. Cyanobacteria are regarded as a potential target for this as antibacterial, antiviral, antifungal, algicide and cytotoxic activities have been reported in these organisms. They have been identified as a new and rich source of bioactive compounds belonging to diversified groups. Radiation in the UV-B range interferes with various metabolic reactions by generating free radicals and active oxygen species. These deleterious compounds are inactivated by antioxidants. Among them are the carotenoids and phycocyanin which protect against photodynamic action in different ways. Stress plays an important role in the production of bioactive metabolites from organisms. Synechococcus spp. PCC7942 was studied for antibacterial activity against various pathogenic bacteria resistant to a number of available antibiotics after being exposed to UV-B radiation. The antibacterial activity of Synechococcus spp. PCC7942 was studied on five potent skin pathogens. The highest antibacterial activity was seen the methanol extracts of 24 h UV-B exposed cultures of Synechococcus spp. PCC7942. It can be concluded that there was moderate antibacterial activity. Results showed stress, solvent and dose-dependent activity. This antibacterial activity might be due to the enhanced synthesis of carotenoids and phycocyanin under UV-B stress. The purpose of the present study was to relate the inhibitory effects of the cyanobacterial compounds specifically on skin pathogens with exposure to UV-B radiation as UV protecting compounds are already reported in these organisms.

  14. Environmental radiation and exposure to radiation

    International Nuclear Information System (INIS)

    1981-02-01

    Compared to 1977 the exposure to radiation of the population of the Federal Republic of Germany from both natural and artificial radiation sources has not greatly charged. The amin part of exposure to natural radiation is caused by environmental radiation and by the absorption of naturally radioactive substances into the body. Artificial exposure to radiation of the population is essentially caused by the use of ionizing rays and radioactive substances in medicine. When radioactive materials are released from nuclear facilities the exposure to radiation of the population is only very slightly increased. The real exposure to radiation of individual people can even in the worst affected places, have been at most fractions of a millirem. The exposure to radiation in the worst afected places in the area of a hard-coal power station is higher than that coming from a nuclear power station of the same capacity. The summation of all contributions to the exposure of radiation by nuclear facilities to the population led in 1978 in the Federal Republic of Germany to a genetically significant dose of clearly less than 1 millerem per year. The medium-ranged exposure to radiation by external radiation effects through professional work was in 1978 at 80 millirems. No difference to 1977. The contribution of radionuclide from the fallout coming from nuclear-weapon tests and which has been deposited in the soil, to the whole-body dose for 1978 applies the same as the genetically significant dose of the population with less than 1 millirem. (orig./HP) [de

  15. Ambient solar UV radiation and seasonal trends in potential sunburn risk among schoolchildren in South Africa

    CSIR Research Space (South Africa)

    Wright, CY

    2011-07-01

    Full Text Available The detrimental effects of excess personal solar ultraviolet (UV) radiation exposure include sunburn, immunosuppression and skin cancer. In South Africa, individuals with minimum natural protection from melanin, including fair-skinned individuals...

  16. On the interaction of UV-B radiation (280-315 mm) with water stress in crop plants

    International Nuclear Information System (INIS)

    Balakumar, T.; Vincent, V.H.B.; Paliwal, K.

    1993-01-01

    Cowpea (Vigna unguiculata L. Walp.) seedlings (3-day-old) were subjected to 4 kinds of experimental treatments: (1) control without exposure to any stress (-D-UV), (2) moderate water stress with no UV-B irradiation (+D-UV), (3) no water stress but exposure to UV-B radiation (-D+UV), and (4) moderate water stress and exposure to UV-B (+D+UV). UV-B and drought stress in the combined form elicited beneficial effects on the morphological and growth characteristics, and a few additive inhibitory effects in some functional processes. An increase in the specific leaf weight (SLW) was observed in the combination of stresses, which could be a defence mechanism against UV-B. The combination of stresses promoted the synthesis of anthocyanins and phenolic compounds. The responses of plants to the combination of stresses indicate that during simultaneous exposure of plants to multiple stresses, one form of stress could minimize the damage by the other. The enhancement of superoxide dismutase (SOD) and catalase activities appear to serve as acclimation mechanisms to scavenge the toxic, free radicals of oxygen produced under stress conditions. However, the inhibition in nitrate metabolism was greater in the combined stresses than in either of the stresses imposed separately. The results of this study illustrate that the interaction of stresses during simultaneous multiple stress conditions brings out certain beneficial effects. (author)

  17. [The impact of UV radiation B and C in vitro on different of bacteria strains isolated from patients hospitalized in the Warsaw Medical University Clinics].

    Science.gov (United States)

    Rongies, Witold; Wultańska, Dorota; Kot, Katarzyna; Bogusz, Aleksandra; Rongies, Magdalena; Świercz, Paweł; Swierszcz, Paweł; Lewandowska, Monika; Cholewińska, Grazyna; Meisel-Mikołajczyk, Felicja

    2011-01-01

    Infections in human body caused by various microbes are a significant problem in modern medicine. Special attention is put to infections of wounds, which are a significant threat to the life of patients. Attempts to treat these wounds base mainly on the application of various chemical preparations (locally) and systematic antibiotic treatment. UV radiation, because of its anti-bacterial activity, appear a complementary issue in therapy. AIM OF THE SURVEY: The aim of this study was an examination of the sensitivity of bacteria strains isolated from patients hospitalised in the Warsaw Medical University clinics, and prove that antibiotics and operation of UV B and C radiation with Endolamp 474 may become a complementary or alternative method of treatment. The study used 65 strains grown aerobically (15 strains of Escherichia coli, 20 strains of Pseudomonas aeruginosa, 15 strains of Staphylococcus aureus, 15 strains of Streptococcus and Enterococcus sp). The same strains were planted on different excipients and were subjected to UV radiation using Endolamp 474. Correctly prepared strains were radiated from a 25 cm distance in various durations (from 5 seconds to 105 seconds). As a result of UV irradiation of microorganisms studied B and C using 474 Endolampy received varied, but the great sensitivity to the effects of this radiation, in all tested bacterial strains. UV radiation on microorganisms requires further study, also in vivo.

  18. Use of UV-protective windows and window films to aid in the prevention of skin cancer.

    Science.gov (United States)

    Edlich, Richard F; Winters, Kathryne L; Cox, Mary Jude; Becker, Daniel G; Horowitz, Jed H; Nichter, Larry S; Britt, L D; Long, William B; Edlic, Elizabeth C

    2004-01-01

    People are exposed to ambient solar ultraviolet (UV) radiation throughout their daily routine, intentionally and unintentionally. Cumulative and excessive exposure to UV radiation is the behavioral cause to skin cancers, skin damage, premature skin aging, and sun-related eye disorders. More than one million new cases of skin cancer were diagnosed in the United States this year. UV radiates directly and diffusely scattered by the various environmental and atmospheric conditions and has access to the skin from all directions. Because of this diffuse UV radiation, a person situated under a covering, such as the roof of a car or house, is not completely protected from the sun's rays. Because shade structures do not protect effectively against UV radiation, there have been major advances in photoprotection of glass by the development of specially designed photoprotective windows and films. It is the purpose of this collective review to highlight the photoprotective windows and films that should be incorporated into residential, commercial, and school glass windows to reduce sun exposure. Low-emittence (low-E) coatings are microscopically thin, virtually invisible, metal or metallic oxide layers deposited on a window or skylight glazing surface to reduce the U-factor by suppressing radiative heat flow as well as to limit UV radiation. The exclusive Thermaflect coating uses the most advanced, double-layer soft coat technology to continue to deliver top performance for UV protection as well as prevent heat loss in the home. This product blocks 87% of UV radiation and has an Energy Star certification in all climate zones. Tints and films have been another important advance in glass photoprotection, especially in automobiles. Quality widow film products are high-tech laminates of polyester and metallized coatings bonded by distortion-free adhesives. The International Window Film Association provides members with accreditation in solar control films, safety films, and

  19. Control of phenylalanine ammonia-lyase gene promoters from pea by UV radiation

    International Nuclear Information System (INIS)

    Pluskota, W.E.; Michalczyk, D.J.; Gorecki, R.J.

    2005-01-01

    The gene fusion system was used to study UV light-control of PS PAL1 and PS PAL2 genes encoding phenylalanine ammonia-lyase of pea. The induction of pea PAL promoters was analysed in transgenic tobacco plants. Binary plasmids (derivatives of pBI101.2 vector) containing 5' regulatory fragments of PS PAL1 and PS PAL2 linked to reporter genes (GUS, LUC) were constructed. The analyses were performed with the use of single constructs (containing one variant of PS PAL promoter and one reporter gene) and dual constructs (containing both PS PAL1 and PS PAL2 promoters connected with different reporter genes). The use of dual constructs enabled the evaluation of both PS PAL promoters activity in the same plant. The analyses of in vitro grown plants have shown that both PAL promoters are strongly induced in leaves subjected to UV radiation. In some cases, the UV-stimulated expression exceeded the exposed areas. This phenomenon was observed more often in the leaves of plants containing the PS PAL1::GUS than PS PAL2::GUS construct. Removal of boxes 2, 4, 5 from PS PAL1 promoter and deletion of its 5' end region (-339 to -1394) decreases the level of gene expression but does not eliminate its responsiveness to UV

  20. UV radiation induced stress does not affect DMSP synthesis in the marine prymnesiophyte Emiliania huxleyi

    NARCIS (Netherlands)

    van Rijssel, M; Buma, A.G.J.

    2002-01-01

    A possible coupling between UV radiation (UVR; 280 to 400 nm) induced stress and the production of dimethylsulfoniopropionate (DMSP), the precursor of the climate-regulating gas dimethylsulfide (DMS), was investigated in the marine prymnesiophyte Emiliania huxleyi. To this end, axenic cultures of E.