WorldWideScience

Sample records for enormous acidifying effect

  1. Effects of acidified drinking water on the teeth of laboratory mice

    OpenAIRE

    Kostov, Marko

    2013-01-01

    The aim of this investigation was to determine the effects of hydrochloric acid used as hygienic measure in drinking water (pH2) on teeth of conventional and germfree laboratory mice. Four groups of 10 animals each were used. Group 1 : Conventional animals (tap water) Group 2 : Conventional animals (tap water, acidified ) Group 3 : Germfree animals (tap water, sterilized ) Group 4 : Germfree animals (tap water , acidified, sterilized ) The application of acidified drinking ...

  2. Fresh and preserved green fodder modify effects of urinary acidifiers on urine pH of horses.

    Science.gov (United States)

    Goren, G; Fritz, J; Dillitzer, N; Hipp, B; Kienzle, E

    2014-04-01

    Hay stabilises urine pH in horses. It is unknown whether this is an effect of structure or of chemical composition. In this study, four ponies (230-384 kg body weight [BW]) were fed six different diets with either a structure or a composition similar to hay with and without acidifiers in a cross-over experimental design in amounts to maintain body weight with the following main compounds: Fresh grass (GRASS), alfalfa hay (ALF), grass cobs (COBS), grass silage (SIL), straw (STR) or extruded straw (STRe) for 2 to 10 days. Urine pH was measured in all trials, blood pH, blood base excess and bicarbonate as well as mineral balance were determined in GRASS, ALF, STR and STRe. In the trials with straw and extruded straw, urine pH decreased significantly (STR control: 7.8 ± 0.23, acidifier: 5.2 ± 0.38) when acidifiers were added, whereas in all other diets that were based on fresh or preserved green fodder, pH did not decrease below 7. Blood pH was similarly affected by diet and acidifiers. Acidifiers had little effect on the pre-prandial blood pH, only in diet STR there was a significant reduction in relation to control. Post-prandial blood pH was significantly reduced by acidifiers in all diets. Blood bicarbonate and base excess showed corresponding effects. Faecal and renal mineral excretion and apparent mineral digestibility were not systematically affected by diet or acidifiers except for chloride. Chloride added as inorganic chloride salt had an even better apparent digestibility than chloride originating from feed. Because only green plant material stabilised acid base balance, chlorophyll and its metabolites are discussed as potential mediators of the effect of green fodder on acid base balance. Journal of Animal Physiology and Animal Nutrition © 2013 Blackwell Verlag GmbH.

  3. Thinner eggshells of dipper (Cinclus cinclus) eggs from an acidified area compared to a non-acidified area in Norway

    Energy Technology Data Exchange (ETDEWEB)

    Nyboe, S.; Staurnes, M.; Jerstad, K. [Norwegian University of Science and Technology, Dragvoll (Norway). Dept. of Zoology

    1997-01-01

    Eggs of dippers Cinclus cinclus from a chronically acidified area in Southern Norway were compared with eggs from a non-acidified area in Central Norway. There were no differences in egg size, as measured by volume, weight, length and calculated surface area, between the two areas. Eggshells were 7.0% lighter and 6.1% thinner, as measured by the Ratcliffe index and 7.0% as measured by the eggshell index (shell weight/surface area) in Southern Norway than in Central Norway. The Ratcliffe and eggshell indices were highly correlated. Scanning electron micrography showed that the palisade layer of eggshells of eggs from the acidified area was 10.7% thinner than that of eggshells of eggs from the non-acidified area. Eggshell vapour permeability was not significantly influenced by area. Since the moderately lower thickness in Southern Norway was not accompanied by higher vapour permeability, this indicates that the reduced eggshell thickness did not cause desiccation of dipper eggs in the acidified area. The possibility of underestimating the environmental effects of acidification on dippers is discussed. 42 refs.,2 figs., 4 tabs.

  4. The Serum Metabolite Response to Diet Intervention with Probiotic Acidified Milk in Irritable Bowel Syndrome Patients Is Indistinguishable from that of Non-Probiotic Acidified Milk by 1H NMR-Based Metabonomic Analysis

    Directory of Open Access Journals (Sweden)

    Ulla Svensson

    2010-11-01

    Full Text Available The effects of a probiotic acidified milk product on the blood serum metabolite profile of patients suffering from Irritable Bowel Syndrome (IBS compared to a non-probiotic acidified milk product was investigated using 1H NMR metabonomics. For eight weeks, IBS patients consumed 0.4 L per day of a probiotic fermented milk product or non-probiotic acidified milk. Both diets resulted in elevated levels of blood serum l-lactate and 3-hydroxybutyrate. Our results showed identical effects of acidified milk consumption independent of probiotic addition. A similar result was previously obtained in a questionnaire-based evaluation of symptom relief. A specific probiotic effect is thus absent both in the patient subjective symptom evaluations and at the blood serum metabolite level. However, there was no correspondence between symptom relief and metabolite response on the patient level.

  5. Effects of simulated rain acidified with sulfuric acid on host-parasite interactions

    Science.gov (United States)

    D. S. Shriner

    1976-01-01

    Wind-blown rain, rain splash, and films of free moisture play important roles in the epidemiology of many plant diseases. The effects of simulated rain acidified with sulfuric acid were studied on several host-parasite systems. Plants were exposed, in greenhouse or field, to simulated rain of pH 3.2 ? 0.1 or pH 6.0 ? 0.2. Simulated "rain" of pH 3.2 resulted...

  6. Stability of endoglucanases from mesophilic fungus and thermophilic bacterium in acidified polyols.

    Science.gov (United States)

    Chong, Barrie Fong; Harrison, Mark D; O'Hara, Ian M

    2014-01-01

    Recent developments in chemical pretreatments of lignocellulosic biomass using polyols as co-solvents (e.g., glycerol and ethylene glycol) at temperatures less than 100°C may allow the effective use of thermostable and non-thermostable cellulases in situ during the saccharification process. The potential of biomass saccharifying enzymes, endoglucanases (EG) from a thermophilic bacterium (Thermotoga maritima) and a mesophilic fungus (Trichoderma longibrachiatum), to retain their activity in aqueous buffer, acidified glycerol, and acidified ethylene glycol used as co-solvents at pretreatment temperatures at or below 100°C were examined. The results show that despite its origin, T. longibrachiatum EG (Tl-EG) retained 75% of its activity after exposure to 100°C for 5 min in aqueous buffer while T. maritima EG (Tm-EG) retained only 5% activity. However, at 90°C both enzymes retained over 87% of their activity. In acidified (0.1% (w/w) H2SO4) glycerol, Tl-EG retained similar activity (80%) to that obtained in glycerol alone, while Tm-EG retained only 35%. With acidified ethylene glycol under these conditions, both Tl-EG and Tm-EG retained 36% of their activity. The results therefore show that Tl-EG is more stable in both acidified glycerol and ethylene glycol than Tm-EG. A preliminary kinetic study showed that pure glycerol improved the thermal stability of Tl-EG but destabilized Tm-EG, relative to the buffer solution. The half-lives of both Tl-EG and Tm-EG are 4.5 min in acidified glycerol, indicating that the effectiveness of these enzymes under typical pretreatment times of greater than 15 min will be considerably diminished. Attempts have been made to explain the differences in the results obtained between the two enzymes. Copyright © 2014 Elsevier Inc. All rights reserved.

  7. Effect of an acidifier as replacement for antibiotics on the ...

    African Journals Online (AJOL)

    An experiment was conducted to determine the influence of an acidifier as an alternative to antibiotics on the performance and gut morphology of broiler chickens. One hundred and eighty (180) 7-day old broiler chicks were randomly assigned to 3 dietary treatments of 4 replicates each. Each replicate had 15 birds.

  8. The Serum Metabolite Response to Diet Intervention with Probiotic Acidified Milk in Irritable Bowel Syndrome Patients is Indistinguishable from that of Non-Probiotic Acidified Milk by 1H NMR-Based Metabonomic Analysis

    DEFF Research Database (Denmark)

    Pedersen, Simon M M; Nielsen, Niels Chr; Andersen, Henrik J

    2010-01-01

    of a probiotic fermented milk product or non-probiotic acidified milk. Both diets resulted in elevated levels of blood serum L-lactate and 3-hydroxybutyrate. Our results showed identical effects of acidified milk consumption independent of probiotic addition. A similar result was previously obtained...

  9. Nebulization of the acidified sodium nitrite formulation attenuates acute hypoxic pulmonary vasoconstriction

    Directory of Open Access Journals (Sweden)

    Surber Mark W

    2010-06-01

    Full Text Available Abstract Background Generalized hypoxic pulmonary vasoconstriction (HPV occurring during exposure to hypoxia is a detrimental process resulting in an increase in lung vascular resistance. Nebulization of sodium nitrite has been shown to inhibit HPV. The aim of this project was to investigate and compare the effects of nebulization of nitrite and different formulations of acidified sodium nitrite on acute HPV. Methods Ex vivo isolated rabbit lungs perfused with erythrocytes in Krebs-Henseleit buffer (adjusted to 10% hematocrit and in vivo anesthetized catheterized rabbits were challenged with periods of hypoxic ventilation alternating with periods of normoxic ventilation. After baseline hypoxic challenges, vehicle, sodium nitrite or acidified sodium nitrite was delivered via nebulization. In the ex vivo model, pulmonary arterial pressure and nitric oxide concentrations in exhaled gas were monitored. Nitrite and nitrite/nitrate were measured in samples of perfusion buffer. Pulmonary arterial pressure, systemic arterial pressure, cardiac output and blood gases were monitored in the in vivo model. Results In the ex vivo model, nitrite nebulization attenuated HPV and increased nitric oxide concentrations in exhaled gas and nitrite concentrations in the perfusate. The acidified forms of sodium nitrite induced higher levels of nitric oxide in exhaled gas and had longer vasodilating effects compared to nitrite alone. All nitrite formulations increased concentrations of circulating nitrite to the same degree. In the in vivo model, inhaled nitrite inhibited HPV, while pulmonary arterial pressure, cardiac output and blood gases were not affected. All nitrite formulations had similar potency to inhibit HPV. The tested concentration of appeared tolerable. Conclusion Nitrite alone and in acidified forms effectively and similarly attenuates HPV. However, acidified nitrite formulations induce a more pronounced increase in nitric oxide exhalation.

  10. Use of multivitamin, acidifier and Azolla in the diet of broiler chickens.

    Science.gov (United States)

    Islam, M A; Nishibori, M

    2017-05-01

    The experiments were carried out to measure the effect of multivitamin, acidifier and Azolla on growth performance, profitability and lipid profiles of blood of broiler chickens to produce safe and cost effective broilers. In experiment 1, 240 day-old Cobb-500 broiler chicks were fed diets; D 1 (control), D 2 (D 1 with 1 mL multivitamin/liter water), D 3 (D 1 with 1 mL acidifier/liter water), D 4 (D 1 with 1 mL multivitamin and 2 mL acidifier/liter water) having 3 replications in each, and 20 chicks/replication. In experiment 2, 150 day-old Cobb-500 broiler chicks were fed diets; T 1 (control), T 2 (5% Azolla in the diet), T 3 (7% Azolla in the diet) and T 4 (T 1 with 1 mL multivitamin and 1 mL acidifier/liter water) having 3 replications in each, and 20 chicks/replication in control, and 10 chicks/replication in the remaining dietary treatment groups for 35 days. In experiment 1, the highest live weight was observed in D 4 (p0.05). The lowest feed conversion ratio (FCR) (p0.05). However, evidently but not significantly, the highest net profit was obtained in D 2 followed by D 4 , D 1 , and D 3 , respectively. In experiment 2, the highest live weight (p0.05). In conclusion, Azolla and acidifier reduced lipid profiles of broiler chickens. Considering net profit and lipid profiles, 5% Azolla may be the suitable dietary group for producing safe and profitable broilers. However, more studies are needed to confirm this study prior to suggesting using Azolla in the poultry industry.

  11. Use of multivitamin, acidifier and Azolla in the diet of broiler chickens

    Directory of Open Access Journals (Sweden)

    M. A. Islam

    2017-05-01

    Full Text Available Objective The experiments were carried out to measure the effect of multivitamin, acidifier and Azolla on growth performance, profitability and lipid profiles of blood of broiler chickens to produce safe and cost effective broilers. Methods In experiment 1, 240 day-old Cobb-500 broiler chicks were fed diets; D1 (control, D2 (D1 with 1 mL multivitamin/liter water, D3 (D1 with 1 mL acidifier/liter water, D4 (D1 with 1 mL multivitamin and 2 mL acidifier/liter water having 3 replications in each, and 20 chicks/replication. In experiment 2, 150 day-old Cobb-500 broiler chicks were fed diets; T1 (control, T2 (5% Azolla in the diet, T3 (7% Azolla in the diet and T4 (T1 with 1 mL multivitamin and 1 mL acidifier/liter water having 3 replications in each, and 20 chicks/replication in control, and 10 chicks/replication in the remaining dietary treatment groups for 35 days. Results In experiment 1, the highest live weight was observed in D4 (p0.05. The lowest feed conversion ratio (FCR (p0.05. However, evidently but not significantly, the highest net profit was obtained in D2 followed by D4, D1, and D3, respectively. In experiment 2, the highest live weight (p0.05. Conclusion In conclusion, Azolla and acidifier reduced lipid profiles of broiler chickens. Considering net profit and lipid profiles, 5% Azolla may be the suitable dietary group for producing safe and profitable broilers. However, more studies are needed to confirm this study prior to suggesting using Azolla in the poultry industry.

  12. The fate of sulfate in acidified pig slurry during storage and following application to cropped soil

    DEFF Research Database (Denmark)

    Eriksen, Jørgen; Sørensen, Peter; Elsgaard, Lars

    2008-01-01

    -available sulfate form. Microbial sulfate reduction during storage of acidified pig slurry was limited, presumably due to initial pH effects and a limitation in the availability of easily degradable organic matter. Sulfide accumulation was observed during storage but the sulfide levels in acidified slurry did...

  13. Intracellular pH homeostasis plays a role in the tolerance ofDebaryomyces hansenii and Candida zeylanoides to acidified nitrite

    DEFF Research Database (Denmark)

    Mortensen, Henrik Dam; Jacobsen, Thomas; Koch, Anette Granly

    2008-01-01

    . hansenii at an external pH (pHex) value of 4.5 butdid not at pHex 5.5. These results indicate that nitrous acid as such plays an important role in the antifungal effect of acidified nitrite. Furthermore, both yeast species experienced severe growth inhibition and a pHi decrease at pHex 4.5, suggesting...... that at least some of the antifungal effects of acidified nitrite may be due to intracellular acidification. For C. zeylanoides, this phenomenon could be explained in part by the uncoupling effect of energy generation from growth. Debaryomyces hansenii was more tolerant to acidified nitrite at pHex 5.5 than C....... zeylanoides, as determined by the rate of growth initiation. In combination with the fact that D. hansenii was able to maintain pHi homeostasis at pHex 5.5 but C. zeylanoides was not, our results suggest that the ability to maintain pHi homeostasis plays a role in the acidified-nitrite tolerance of D...

  14. Influence of acidified drinking water on growth performance and gastrointestinal function of broilers.

    Science.gov (United States)

    Hamid, H; Shi, H Q; Ma, G Y; Fan, Y; Li, W X; Zhao, L H; Zhang, J Y; Ji, C; Ma, Q G

    2018-06-01

    The ban on the use of antibiotic feed additives as growth promoters compelled the researchers for exploring the future utility of other alternatives. This experiment was designed to evaluate the effect of acidified drinking water on growth performance, gastrointestinal pH, digestive enzymes, intestinal histomorphology, and cecum microbial counting of the broiler chicken. A total of 540 one-day-old male broilers (Arbor Acre) were randomly assigned to 5 treatments, with 6 replicates of 18 chicks per replicate. Broilers received diets and water as follows: NC (negative control, basal diet, normal water), PC (positive control, basal diet + 8 ppm colistin sulfate + 8 ppm enduracidin, normal water), A1 (basal diet, continuous supply of acidified water during whole experiment period), A2 (basal diet, intermittent acidification of water during 0 to 14 d, 22 to 28 d, and 36 to 42 d), and A3 [basal diet, intermittent acidification of water (24 h/d from 0 to 14 d and from 10:00 am to 4:00 pm on d 15 to 42)]. During the entire period, the acidified groups (A1, A2, and A3) and PC group showed improve on weight gain, average daily gain and feed conversion ratio compared to NC group (P < 0.05). The pH in crop, proventriculus and ileum at 43 d declined by 0.04, 1.03, 1.23; 0.55, 0.69, 0.70; and 0.63, 0.74, 1.21 in A1, A2, and A3 group, respectively. There was a significant decline of lipase activity in the PC and acidified groups compared to NC group. The A2 group had higher villus height in jejunum than NC group. The PC and acidified groups reduced (P < 0.05) the total aerobic bacteria count of cecum when contrasted to NC group. Therefore, we conclude that acidified drinking water can improve growth performance, compensate for gastric acidity, and control pathogenic bacteria in broilers and may be considered as a potential alternative to improve production parameters. Discontinuous supply of acidified water had the same or even better influence on broilers compared to

  15. Statistical modeling/optimization and process intensification of microwave-assisted acidified oil esterification

    International Nuclear Information System (INIS)

    Ma, Lingling; Lv, Enmin; Du, Lixiong; Lu, Jie; Ding, Jincheng

    2016-01-01

    Highlights: • Microwave irradiation was employed for the esterification of acidified oil. • Optimization and modeling of the process was performed by RSM and ANN. • Both models have reliable prediction abilities but the ANN was superior over the RSM. • Membrane vapor permeation and in-situ dehydration were used to shift the equilibrium. • Two dehydration approaches improved the FFAs conversion rate by 20.0% approximately. - Abstract: The esterification of acidified oil with ethanol under microwave radiation was modeled and optimized using response surface methodology (RSM) and artificial neural network (ANN). The impacts of mass ratio of ethanol to acidified oil, catalyst loading, microwave power and reaction time are evaluated by Box-Behnken design (BBD) of RSM and multi-layer perceptron (MLP) of ANN. RSM combined with BBD shows the optimal conditions as catalyst loading of 5.85 g, mass ratio of ethanol to acidified oil of 0.35 (20.0 g acidified oil), microwave power of 328 W and reaction time of 98.0 min with the free fatty acids (FFAs) conversion of 78.57%. Both of the models are fitted well with the experimental data, however, ANN exhibits better prediction accuracy than RSM based on the statistical analyses. Furthermore, membrane vapor permeation and in-situ molecular sieve dehydration were investigated to enhance the esterification under the optimized conditions.

  16. Eubiotic effect of a dietary acidifier (potassium diformate) on the health status of cultured Oreochromis niloticus

    Science.gov (United States)

    Abu Elala, Nermeen M.; Ragaa, Naela M.

    2014-01-01

    In connection with the global demand for safe human food and the production of environmentally friendly aquaculture products, acidifiers are natural organic acids and salts that have received considerable attention as animal-feed additives. The current study was designed to evaluate the effects of potassium diformate (KDF) on the growth performance and immunity of cultured Oreochromis niloticus (O. niloticus). Four iso-nitrogenous and iso-caloric rations containing graded levels of KDF, including 0% (control basal diet), 0.1%, 0.2% and 0.3%, were fed separately to four equal fish groups (30 fish/group with an initial body weight of 53.49 ± 6.15 g) for sixty days. At the end of the experimental period, the fish groups fed on 0.2% and 0.3% KDF exhibited significant improvements in their feed intake, live weight gain, specific growth rate, feed conversion ratio and protein efficiency ratio, with concomitant improvement of their apparent protein digestibility (p fish groups fed on KDF and challenged orally with Aeromonas hydrophila was lower than that of the control group. The resistance against diseases increased with dietary KDF in a dose-dependent manner. Thus, we conclude that the use of acidifiers can be an efficient tool to achieve sustainable, economical and safe fish production. PMID:26199753

  17. 21 CFR 108.25 - Acidified foods.

    Science.gov (United States)

    2010-04-01

    ... Administration on Form FDA 2541 (food canning establishment registration) information including, but not limited... continuous inspection of the meat and poultry inspection program of the Food Safety and Inspection Service of... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Acidified foods. 108.25 Section 108.25 Food and...

  18. Deposition of acidifying compounds

    International Nuclear Information System (INIS)

    Fowler, D.; Cape, J.N.; Sutton, M.A.; Mourne, R.; Hargreaves, K.J.; Duyzer, J.H.; Gallagher, M.W.

    1992-01-01

    Inputs of acidifying compounds to terrestrial ecosystems include deposition of the gases NO 2 , NO, HNO 2 , HNO 3 , NH 3 and SO 2 and the ions NO 3- , NH 4+ , SO 4 2- and H + in precipitation, cloud droplets and particles. Recent research has identified particular ecosystems and regions in which terrestrial effects are closely linked with specific deposition processes. This review paper identifies areas in which important developments have occurred during the last five years and attempts to show which aspects of the subject are most important for policy makers. Amongst the conclusions drawn, the authors advise that current uncertainties in estimates of S and N inputs by dry deposition should be incorporated in critical load calculations, and that, in regions dominated by wet deposition, spatial resolution of total inputs should be improved to match the current scales of information on landscape sensitivity to acidic inputs. 44 refs., 9 figs

  19. Administration of acidified drinking water to finishing pigs in order to prevent Salmonella infections.

    Science.gov (United States)

    van der Wolf, P J; van Schie, F W; Elbers, A R; Engel, B; van der Heijden, H M; Hunneman, W A; Tielen, M J

    2001-07-01

    The aim of the study was to test whether acidified drinking water, with two millilitres of an acid mixture per litre, was able to reduce the number of Salmonella infections in finishing pig herds. In each compartment, half of the pens were supplied with acidified water and the other pens served as negative control. In three herds the required dose was not applied to the pigs as a result of various practical problems. In another herd, all pigs remained seronegative throughout the study. Analysis of the remaining three herds showed a large and significant treatment effect in one herd (Pdrinking nipples as a result of fungal growth in the pipelines.

  20. Effects of exogenous salinity (NaCl) gradient on Cd release in acidified contaminated brown soil

    Science.gov (United States)

    Zhang, Lina; Rong, Yong; Mao, Li; Gao, Zhiyuan; Liu, Xiaoyu; Dong, Zhicheng

    2018-02-01

    Taking acidified Cd contaminated brown soil in Yantai as the research object, based on different exogenous salinity (NaCl) gradient (0%, 0.3%, 0.6%, 0.9%, 1.5%, 2% and 5%), indoor simulation experiments of Cd release were carried out after field investigation. Results showed that there was a significantly positive relation (r>0.90) between Cd release concentration/amount/ratio and exogenous salt (NaCl). Besides, the more exogenous salt (NaCl) was added; maximum release concentration/amount of Cd appeared the earlier. It was found that exogenous salt (NaCl) addition could obviously promote Cd release from acidified Cd contaminated brown soil. It was believed that this could be mainly due to the cation exchange between Cd2+ and Na+, together with the dissociation and/or complexation between Cl- and Cd2+. In addition, available adsorption sites reduction by exchange base in soil causing Cd changed from solid state to soil solution was also a probable reason.

  1. Post-Exercise Muscle Protein Synthesis in Rats after Ingestion of Acidified Bovine Milk Compared with Skim Milk.

    Science.gov (United States)

    Nakayama, Kyosuke; Kanda, Atsushi; Tagawa, Ryoichi; Sanbongi, Chiaki; Ikegami, Shuji; Itoh, Hiroyuki

    2017-09-27

    Bovine milk proteins have a low absorption rate due to gastric acid-induced coagulation. Acidified milk remains liquid under acidic conditions; therefore, the absorption rate of its protein may differ from that of untreated milk. To investigate how this would affect muscle protein synthesis (MPS), we compared MPS after ingestion of acidified versus skim milk in rats. Male Sprague-Dawley rats swam for 2 h and were immediately administered acidified or skim milk, then euthanized at 30, 60, 90, and 120 min afterwards. Triceps muscle samples were excised for assessing fractional synthetic rate (FSR), plasma components, intramuscular free amino acids and mTOR signaling. The FSR in the acidified milk group was significantly higher than in the skim milk group throughout the post-ingestive period. Plasma essential amino acids, leucine, and insulin levels were significantly increased in the acidified milk group at 30 min after administration compared to the skim milk group. In addition, acidified milk ingestion was associated with greater phosphorylation of protein kinase B (Akt) and ribosomal protein S6 kinase (S6K1), and sustained phosphorylation of 4E-binding protein 1 (4E-BP1). These results indicate that compared with untreated milk, acidified milk ingestion is associated with greater stimulation of post-exercise MPS.

  2. Post-Exercise Muscle Protein Synthesis in Rats after Ingestion of Acidified Bovine Milk Compared with Skim Milk

    Directory of Open Access Journals (Sweden)

    Kyosuke Nakayama

    2017-09-01

    Full Text Available Bovine milk proteins have a low absorption rate due to gastric acid-induced coagulation. Acidified milk remains liquid under acidic conditions; therefore, the absorption rate of its protein may differ from that of untreated milk. To investigate how this would affect muscle protein synthesis (MPS, we compared MPS after ingestion of acidified versus skim milk in rats. Male Sprague-Dawley rats swam for 2 h and were immediately administered acidified or skim milk, then euthanized at 30, 60, 90, and 120 min afterwards. Triceps muscle samples were excised for assessing fractional synthetic rate (FSR, plasma components, intramuscular free amino acids and mTOR signaling. The FSR in the acidified milk group was significantly higher than in the skim milk group throughout the post-ingestive period. Plasma essential amino acids, leucine, and insulin levels were significantly increased in the acidified milk group at 30 min after administration compared to the skim milk group. In addition, acidified milk ingestion was associated with greater phosphorylation of protein kinase B (Akt and ribosomal protein S6 kinase (S6K1, and sustained phosphorylation of 4E-binding protein 1 (4E-BP1. These results indicate that compared with untreated milk, acidified milk ingestion is associated with greater stimulation of post-exercise MPS.

  3. Administration of acidified drinking water to finishing pigs in order to prevent Salmonella infections

    NARCIS (Netherlands)

    Wolf, van der P.J.; Schie, van F.W.; Elbers, A.R.W.; Engel, B.; Heijden, van der H.; Hunneman, W.A.; Tielen, M.J.M.

    2001-01-01

    The aim of the study was to test whether acidified drinking water, with two millilitres of an acid mixture per litre, was able to reduce the number of Salmonella infections in finishing pig herds. In each compartment, half of the pens were supplied with acidified water and the other pens served as

  4. Defining reference conditions for acidified waters using a modern analogue approach

    International Nuclear Information System (INIS)

    Simpson, Gavin L.; Shilland, Ewan M.; Winterbottom, Julie M.; Keay, Janey

    2005-01-01

    Analogue matching is a palaeolimnological technique that aims to find matches for fossil sediment samples from a set of modern surface sediment samples. Modern analogues were identified that closely matched the pre-disturbance conditions of eight of the UK Acid Waters Monitoring Network (AWMN) lakes using diatom- and cladoceran-based analogue matching. These analogue sites were assessed in terms of hydrochemistry, aquatic macrophytes and macro-invertebrates as to their suitability for defining wider hydrochemical and biological reference conditions for acidified sites within the AWMN. The analogues identified for individual AWMN sites show a close degree of similarity in terms of their hydrochemical characteristics, aquatic macrophytes and, to a lesser extent, macro-invertebrate fauna. The reference conditions of acidified AWMN sites are inferred to be less acidic than today and to support a wider range of acid-sensitive aquatic macrophyte and macro-invertebrate taxa than that recorded in the AWMN lakes over the period of monitoring since 1988. - The use of a palaeolimnological technique to identify modern ecological reference analogues for acidified lakes is demonstrated

  5. Effects of feeding untreated, pasteurized and acidified waste milk and bunk tank milk on the performance, serum metabolic profiles, immunity, and intestinal development in Holstein calves.

    Science.gov (United States)

    Zou, Yang; Wang, Yajing; Deng, Youfei; Cao, Zhijun; Li, Shengli; Wang, Jiufeng

    2017-01-01

    The present experiment was performed to assess the effects of different sources of milk on the growth performance, serum metabolism, immunity, and intestinal development of calves. Eighty-four Holstein male neonatal calves were assigned to one of the following four treatment groups: those that received bunk tank milk (BTM), untreated waste milk (UWM), pasteurized waste milk (PWM), and acidified waste milk (AWM) for 21 d. Calves in the BTM and AWM groups consumed more starter ( P  feeding on BTM had lower ( P  waste milk. The efficiency of feeding pasteurized and acidified waste milk are comparable, and the acidification of waste milk is an acceptable labor-saving and diarrhea-preventing feed for young calves.

  6. Probabilistic risk assessment of the effect of acidified seawater on development stages of sea urchin (Strongylocentrotus droebachiensis).

    Science.gov (United States)

    Chen, Wei-Yu; Lin, Hsing-Chieh

    2018-05-01

    Growing evidence indicates that ocean acidification has a significant impact on calcifying marine organisms. However, there is a lack of exposure risk assessments for aquatic organisms under future environmentally relevant ocean acidification scenarios. The objective of this study was to investigate the probabilistic effects of acidified seawater on the life-stage response dynamics of fertilization, larvae growth, and larvae mortality of the green sea urchin (Strongylocentrotus droebachiensis). We incorporated the regulation of primary body cavity (PBC) pH in response to seawater pH into the assessment by constructing an explicit model to assess effective life-stage response dynamics to seawater or PBC pH levels. The likelihood of exposure to ocean acidification was also evaluated by addressing the uncertainties of the risk characterization. For unsuccessful fertilization, the estimated 50% effect level of seawater acidification (EC50 SW ) was 0.55 ± 0.014 (mean ± SE) pH units. This life stage was more sensitive than growth inhibition and mortality, for which the EC50 values were 1.13 and 1.03 pH units, respectively. The estimated 50% effect levels of PBC pH (EC50 PBC ) were 0.99 ± 0.05 and 0.88 ± 0.006 pH units for growth inhibition and mortality, respectively. We also predicted the probability distributions for seawater and PBC pH levels in 2100. The level of unsuccessful fertilization had 50 and 90% probability risks of 5.07-24.51 (95% CI) and 0-6.95%, respectively. We conclude that this probabilistic risk analysis model is parsimonious enough to quantify the multiple vulnerabilities of the green sea urchin while addressing the systemic effects of ocean acidification. This study found a high potential risk of acidification affecting the fertilization of the green sea urchin, whereas there was no evidence for adverse effects on growth and mortality resulting from exposure to the predicted acidified environment.

  7. Transboundary air pollution in Europe. Part 2: Numerical addendum to emissions, dispersion and trends of acidifying and eutrophying agents

    Energy Technology Data Exchange (ETDEWEB)

    Berge, Erik [ed.

    1997-12-31

    This report was prepared for the twenty first session of the Steering Body of EMEP (Co-operative Programme for Monitoring and Evaluation of the Long-Range Transmission of Air Pollutants in Europe). It presents the numerical fields and budgets of the acidifying and eutrophying air pollution in the form of three appendices: annual average air concentrations of acidifying and eutrophying species, 1996; country-to-country deposition budgets for acidifying/eutrophying air pollutants, 1985-95; and grid square deposition of acidifying/eutrophying components allocated to emitting countries, mean 1985-95. 19 figs.

  8. Optimization of acidified oil esterification catalyzed by sulfonated cation exchange resin using response surface methodology

    International Nuclear Information System (INIS)

    Ma, Lingling; Han, Ying; Sun, Kaian; Lu, Jie; Ding, Jincheng

    2015-01-01

    Highlights: • As lipid source, acidified oil are from industrial wastes for renewable energy. • The predicted conversion rate of FFAs was 75.24% under the RSM optimized conditions. • The adsorption system was employed to remove the water produced to shift the equilibrium toward ethyl ester production. • Maximum conversion rate of 98.32% was obtained using adsorption system at optimum process parameters. • Compared with tradition methods, molecular sieve dehydration method improved the conversion rate by 23.08%. - Abstract: The esterification of acidified oil with ethanol catalyzed by sulfonated cation exchange resins (SCER) was optimized using the response surface methodology (RSM). The effects of the molar ratio of ethanol to acidified oil, reaction time and catalyst loading on the conversion rate of free fatty acids (FFAs) were investigated at the temperature of the boiling point of ethanol. Results showed that the highest conversion rate of 75.24% was obtained at the molar ratio of ethanol to acidified oil of 23.2, reaction time of 8.0 h and catalyst loading of 35.0 wt.%. Moreover, the conversion rate of FFAs was increased to 98.32% by using a water adsorption apparatus under the RSM optimized conditions. Scanning electronic microscopic–energy dispersive spectrometric (SEM–EDS), X-ray diffractometric (XRD) and thermogravimetric–derivative thermogravimetric (TG–DTG) analyses confirmed that the morphology of catalysts did not change much and the mechanical and thermal stabilities were still good after the reaction. Furthermore, SCER exhibited a high catalytic activity and stability after being reused for five successive times. The fuel properties of the biodiesel were comparable to that of ASTM, EN and GB biodiesel standard

  9. Effects of acidifying pig diets on emissions of ammonia, methane and sulfur from slurry during storage

    DEFF Research Database (Denmark)

    Eriksen, Jørgen; Nørgaard, Jan Værum; Poulsen, Hanne Damgaard

    2014-01-01

    and feces were collected separately from twenty-four pigs fed one of four diets (Control, +BA, +CaCl2, +BA+CaCl2) in metabolic cages, and mixed as slurry. During 103 days of storage, all acidifying diets consistently reduced pH in the slurry by 0.4 - 0.6 units. There was a strong relationship between slurry...

  10. Eubiotic effect of a dietary acidifier (potassium diformate on the health status of cultured Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Nermeen M. Abu Elala

    2015-07-01

    Full Text Available In connection with the global demand for safe human food and the production of environmentally friendly aquaculture products, acidifiers are natural organic acids and salts that have received considerable attention as animal-feed additives. The current study was designed to evaluate the effects of potassium diformate (KDF on the growth performance and immunity of cultured Oreochromis niloticus (O. niloticus. Four iso-nitrogenous and iso-caloric rations containing graded levels of KDF, including 0% (control basal diet, 0.1%, 0.2% and 0.3%, were fed separately to four equal fish groups (30 fish/group with an initial body weight of 53.49 ± 6.15 g for sixty days. At the end of the experimental period, the fish groups fed on 0.2% and 0.3% KDF exhibited significant improvements in their feed intake, live weight gain, specific growth rate, feed conversion ratio and protein efficiency ratio, with concomitant improvement of their apparent protein digestibility (p < 0.05. Dietary supplementation of 0.3% KDF appeared to stimulate the beneficial intestinal flora; a proliferation was observed of indigenous probionts (Eubiosis associated with the relative activation of cellular and humeral innate immunity (phagocytic activity/index, nitroblue tetrazolium reduction test and serum/gut mucous lysozyme activity. The cumulative mortality of the fish groups fed on KDF and challenged orally with Aeromonas hydrophila was lower than that of the control group. The resistance against diseases increased with dietary KDF in a dose-dependent manner. Thus, we conclude that the use of acidifiers can be an efficient tool to achieve sustainable, economical and safe fish production.

  11. Development of an Effective Chain Elongation Process From Acidified Food Waste and Ethanol Into n-Caproate

    Directory of Open Access Journals (Sweden)

    Mark Roghair

    2018-04-01

    Full Text Available Introduction: Medium chain fatty acids (MCFAs, such as n-caproate, are potential valuable platform chemicals. MCFAs can be produced from low-grade organic residues by anaerobic reactor microbiomes through two subsequent biological processes: hydrolysis combined with acidogenesis and chain elongation. Continuous chain elongation with organic residues becomes effective when the targeted MCFA(s are produced at high concentrations and rates, while excessive ethanol oxidation and base consumption are limited. The objective of this study was to develop an effective continuous chain elongation process with hydrolyzed and acidified food waste and additional ethanol.Results: We fed acidified food waste (AFW and ethanol to an anaerobic reactor while operating the reactor at long (4 d and at short (1 d hydraulic retention time (HRT. At long HRT, n-caproate was continuously produced (5.5 g/L/d at an average concentration of 23.4 g/L. The highest n-caproate concentration was 25.7 g/L which is the highest reported n-caproate concentration in a chain elongation process to date. Compared to short HRT (7.1 g/L n-caproate at 5.6 g/L/d, long HRT resulted in 6.2 times less excessive ethanol oxidation. This led to a two times lower ethanol consumption and a two times lower base consumption per produced MCFA at long HRT compared to short HRT.Conclusions: Chain elongation from AFW and ethanol is more effective at long HRT than at short HRT not only because it results in a higher concentration of MCFAs but also because it leads to a more efficient use of ethanol and base. The HRT did not influence the n-caproate production rate. The obtained n-caproate concentration is more than twice as high as the maximum solubility of n-caproic acid in water which is beneficial for its separation from the fermentation broth. This study does not only set the record on the highest n-caproate concentration observed in a chain elongation process to date, it notably demonstrates that

  12. Complex responses of intertidal molluscan embryos to a warming and acidifying ocean in the presence of UV radiation.

    Directory of Open Access Journals (Sweden)

    Andrew R Davis

    Full Text Available Climate change and ocean acidification will expose marine organisms to synchronous multiple stressors, with early life stages being potentially most vulnerable to changing environmental conditions. We simultaneously exposed encapsulated molluscan embryos to three abiotic stressors-acidified conditions, elevated temperate, and solar UV radiation in large outdoor water tables in a multifactorial design. Solar UV radiation was modified with plastic filters, while levels of the other factors reflected IPCC predictions for near-future change. We quantified mortality and the rate of embryonic development for a mid-shore littorinid, Bembicium nanum, and low-shore opisthobranch, Dolabrifera brazieri. Outcomes were consistent for these model species with embryos faring significantly better at 26°C than 22°C. Mortality sharply increased at the lowest temperature (22°C and lowest pH (7.6 examined, producing a significant interaction. Under these conditions mortality approached 100% for each species, representing a 2- to 4-fold increase in mortality relative to warm (26°C non-acidified conditions. Predictably, development was more rapid at the highest temperature but this again interacted with acidified conditions. Development was slowed under acidified conditions at the lowest temperature. The presence of UV radiation had minimal impact on the outcomes, only slowing development for the littorinid and not interacting with the other factors. Our findings suggest that a warming ocean, at least to a threshold, may compensate for the effects of decreasing pH for some species. It also appears that stressors will interact in complex and unpredictable ways in a changing climate.

  13. Complex Responses of Intertidal Molluscan Embryos to a Warming and Acidifying Ocean in the Presence of UV Radiation

    Science.gov (United States)

    Davis, Andrew R.; Coleman, Daniel; Broad, Allison; Byrne, Maria; Dworjanyn, Symon A.; Przeslawski, Rachel

    2013-01-01

    Climate change and ocean acidification will expose marine organisms to synchronous multiple stressors, with early life stages being potentially most vulnerable to changing environmental conditions. We simultaneously exposed encapsulated molluscan embryos to three abiotic stressors—acidified conditions, elevated temperate, and solar UV radiation in large outdoor water tables in a multifactorial design. Solar UV radiation was modified with plastic filters, while levels of the other factors reflected IPCC predictions for near-future change. We quantified mortality and the rate of embryonic development for a mid-shore littorinid, Bembicium nanum, and low-shore opisthobranch, Dolabrifera brazieri. Outcomes were consistent for these model species with embryos faring significantly better at 26°C than 22°C. Mortality sharply increased at the lowest temperature (22°C) and lowest pH (7.6) examined, producing a significant interaction. Under these conditions mortality approached 100% for each species, representing a 2- to 4-fold increase in mortality relative to warm (26°C) non-acidified conditions. Predictably, development was more rapid at the highest temperature but this again interacted with acidified conditions. Development was slowed under acidified conditions at the lowest temperature. The presence of UV radiation had minimal impact on the outcomes, only slowing development for the littorinid and not interacting with the other factors. Our findings suggest that a warming ocean, at least to a threshold, may compensate for the effects of decreasing pH for some species. It also appears that stressors will interact in complex and unpredictable ways in a changing climate. PMID:23405238

  14. Probiotic yogurt and acidified milk similarly reduce postprandial inflammation and both alter the gut microbiota of healthy, young men.

    Science.gov (United States)

    Burton, Kathryn J; Rosikiewicz, Marta; Pimentel, Grégory; Bütikofer, Ueli; von Ah, Ueli; Voirol, Marie-Jeanne; Croxatto, Antony; Aeby, Sébastien; Drai, Jocelyne; McTernan, Philip G; Greub, Gilbert; Pralong, François P; Vergères, Guy; Vionnet, Nathalie

    2017-05-01

    Probiotic yogurt and milk supplemented with probiotics have been investigated for their role in 'low-grade' inflammation but evidence for their efficacy is inconclusive. This study explores the impact of probiotic yogurt on metabolic and inflammatory biomarkers, with a parallel study of gut microbiota dynamics. The randomised cross-over study was conducted in fourteen healthy, young men to test probiotic yogurt compared with milk acidified with 2 % d-(+)-glucono-δ-lactone during a 2-week intervention (400 g/d). Fasting assessments, a high-fat meal test (HFM) and microbiota analyses were used to assess the intervention effects. Baseline assessments for the HFM were carried out after a run-in during which normal milk was provided. No significant differences in the inflammatory response to the HFM were observed after probiotic yogurt compared with acidified milk intake; however, both products were associated with significant reductions in the inflammatory response to the HFM compared with the baseline tests (assessed by IL6, TNFα and chemokine ligand 5) (Pyogurt intake (FC=-1·3, P adj=0·03), increased abundance of Bifidobacterium species after acidified milk intake (FC=1·4, P adj=0·04) and detection of Lactobacillus delbrueckii spp. bulgaricus (FC=7·0, P adjyogurt intake. Probiotic yogurt and acidified milk similarly reduce postprandial inflammation that is associated with a HFM while inducing distinct changes in the gut microbiota of healthy men. These observations could be relevant for dietary treatments that target 'low-grade' inflammation.

  15. Classifying Your Food as Acid, Low-Acid, or Acidified

    OpenAIRE

    Bacon, Karleigh

    2012-01-01

    As a food entrepreneur, you should be aware of how ingredients in your product make the food look, feel, and taste; as well as how the ingredients create environments for microorganisms like bacteria, yeast, and molds to survive and grow. This guide will help you classifying your food as acid, low-acid, or acidified.

  16. Acidification policy - control of acidifying emissions in Germany

    International Nuclear Information System (INIS)

    Schaerer, B.

    1992-01-01

    Since the mid-eighties total annual acidifying emissions have started to decline in West Germany. There was considerable impact on this positive trend in air pollution by the control of SO 2 and NO x emissions from large boilers, which were reduced by more than 80%. Corresponding control programmes have been established for other groups of sources as well as other pollutants and - with unification - for East Germany. The driving force behind this development was and still is first of all the legal principle of anticipatory action or precaution which means in practical terms 'emission minimization'. This cornerstone of German clean air legislation is the most powerful components of Germany's 'acidification policy', as it requires policy-makers to draw up new or review existing regulations for emission reduction based on requirements according to the state of the art and forces operators to apply the most modern ways and means of operation. This paper describes the system used in Germany to deal with air pollution, the emission minimization strategy, and the actions against acidifying emissions based thereon. In addition, an outlook on what might be necessary to cope with the challenges of a sustainable development concerning acidification is given. 1 ref., 1 fig., 2 tabs

  17. Terrestrial liming benefits birds in an acidified forest in the northeast.

    Science.gov (United States)

    Pabian, Sarah E; Brittingham, Margaret C

    2007-12-01

    Studies in Europe have reported negative effects of acid deposition on forest birds, and research in North America has identified links between forest bird abundance and rates of acid deposition. We examined the bird community in an acidified forest in central Pennsylvania (USA) and evaluated the effects of terrestrial lime application on birds. We used a before-after control-impact (BACI) study design, with one year of observation before (2003) and three years after lime application (2004, 2005, and 2006). Between the 2003 and 2004 field seasons, 4500 kg/ha of dolomitic lime were applied to two of four 100-ha watersheds. Each year, we monitored bird abundance and Ovenbird (Seiurus aurocapilla) eggshell thickness and territory size. Soil and snail abundance data were also collected. The bird community and territory size results indicated that the study area may be providing low-quality habitat for forest birds, perhaps as a result of acid deposition. We found lower forest bird abundances than have been found in less acidified areas of Pennsylvania, and larger Ovenbird territory sizes than have been found in other studies. We found a significant positive relationship between soil calcium and bird abundance, indicating that soil calcium may affect bird abundance. Liming increased soil calcium and pH and led to increased snail and bird abundances. After liming, bird abundance was positively related to snail abundance. No significant changes occurred in Ovenbird territory size or eggshell thickness. Our results suggest that acid deposition could be responsible for reduced bird abundance, and that liming is a potential mitigation technique.

  18. 75 FR 59268 - Draft Guidance for Industry: Acidified Foods; Availability

    Science.gov (United States)

    2010-09-27

    ... fermented dairy products, such as yogurt, belong to a separate category that is not relevant to the... the draft guidance, processors of non-acidified foods (e.g., some acid foods or fermented foods) who... regulations. Under the draft guidance, processors of acid foods and fermented foods who conclude that such...

  19. Biodiesel Production from Acidified Oils via Supercritical Methanol

    Directory of Open Access Journals (Sweden)

    Jianxin Li

    2011-12-01

    Full Text Available In biodiesel production, the vegetable oil used as raw material for transesterification should be free of water and free fatty acids (FFAs, which may consume catalyst and reduce catalyst efficiency. In this work biodiesel was prepared from acidified oils (AO through a supercritical methanol route, in which the esterification of FFAs and transesterification of glyceride with methanol occurred simultaneously. The effects of the mass ratio of methanol to AO, the operation temperature as well as the water content on the FFAs conversion and glycerol yield were investigated. The results indicated that the FFAs conversion for esterification under the condition of 1:1 methanol/oil ratio, 310 °C and 15 min reaction time reached 98.7%, and the glycerol yield for transesterification under 0.25:1 methanol/oil ratio, 290 °C and 20 min reaction time reached 63.5% respectively.

  20. Influence of acidified acidity to uranium bioleaching

    International Nuclear Information System (INIS)

    Li Jiang; Liu Yajie; Zheng Zhihong; Yuan Baohua; Shen Chuan; Shi Weijun

    2012-01-01

    The relationship between the acidified acidity and the acid consumption and uranium leaching rate in the process of uranium bioleaching is investigated. Results indicate that higher uranium leaching rate is obtained when the relatively high acidity was applied at beginning. For different minerals, although the original acidity should be different, lower original acidity was not better for shortening leaching period and improving uranium leaching rate. It confirms 30-40 g/L sulfuric acid as the original acidity was more suitable and more than 30 g/ L should be applied if the mineral particle sizes were larger. (authors)

  1. Relationship of a Special Acidified Milk Protein Drink with Cognitive Performance: A Randomized, Double-Blind, Placebo-Controlled, Crossover Study in Healthy Young Adults

    Directory of Open Access Journals (Sweden)

    Yoshie Saito

    2018-05-01

    Full Text Available A previous in vivo study with rats suggested that a special milk protein drink manufactured using an acidification procedure to suppress the aggregation of milk proteins was absorbed quickly after feeding. We performed a randomized, double-blind, placebo-controlled, repeated-measure crossover study to investigate the short-term effects on cognitive performance in 29 healthy young adult men after they consumed this drink in the morning. After an overnight fast, subjects were tested for performance in the Uchida–Kraepelin serial arithmetic test and the Stroop test as well as for subjective feeling, body temperature, and heart rate variability before and after consumption of either the acidified milk protein drink or an isoenergetic placebo drink. Subjects showed a significant improvement in performance in the Uchida–Kraepelin test, the primary outcome measured, when they consumed the acidified milk protein drink compared with the placebo control condition. In addition, consumption of the acidified milk protein drink, compared with the placebo control, was associated with increases in vagally-mediated heart rate variability indices which, from recent theoretical perspectives, may reflect a higher ability to modulate cognitive and behavioral processes. There was no significant difference in subjective feelings and body temperature between the test drink conditions. These data suggest that consumption of the acidified milk protein drink may improve cognitive performance, with possible involvement of physiological systems that regulate cognition and behavior.

  2. Efeito da utilização de oligossacarídeo manose e acidificantes sobre o desempenho de coelhos em crescimento Effect of the utilization of oligosaccharide mannose and acidifiers on growing rabbits performance

    Directory of Open Access Journals (Sweden)

    Claudio Scapinello

    2001-07-01

    Full Text Available Foi conduzido um experimento com o objetivo de se determinar os efeitos da adição às rações de ácido fumárico, Oligossacarídeo manose (BIO-MOS®, acidificante (ACID-ALL® e a combinação de oligossacarídeo com acidificantes sobre o desempenho de coelhos em crescimento. Foram utilizados 100 coelhos da raça Nova Zelândia Branco, 50 machos e 50 fêmeas, de 39 a 75 dias de idade, distribuídos em um delineamento experimental inteiramente casualizado com cinco tratamentos e 10 repetições e dois animais por unidade experimental. Considerando-se os períodos analisados 39-50 dias de idade e 39-75 dias, nenhuma das características de desempenho estudada foi afetada pela adição de oligossacarídeo, acidificantes ou a combinação de ambos.A trial was carried out to verify the effects of the addition of fumaric acid, oligosaccharide mannose (BIO-MOS®, acidifiers (ACID-ALL® and oligosaccharide + acidifiers in diets growing rabbits on performance. One hundred NZW rabbits, 50 males and 50 females from 39 to 75 days old, distributed in a randomized experimental design with five treatments, 10 replications and two animals by experimental unit. In the period from 39-50 and 39-75 days of experiment none of these performance characteristics was affected by inclusion of oligosaccharide mannose, acidifiers and oligosaccharide +acidifiers did not influence the performance of growing rabbits.

  3. Quality evaluation of packaged acidified vegetables subjected to continuous microwave pasteurization

    Science.gov (United States)

    The study evaluated the use of 915 MHz continuous microwave processing with a rotation apparatus for pasteurization of acidified vegetable packages. Broccoli florets, and 1.2 cm cubes of broccoli stems, red bell pepper, and sweetpotato were pre-equilibrated to 1 g/100 g NaCl and 0.38 g/100 mL citric...

  4. Effects of Feeding Acidified Milk Replacer on the Growth, Health and Behavioural Characteristics of Holstein Friesian Calves

    OpenAIRE

    YANAR, Mete; GÜLER, Olcay; BAYRAM, Bahri; METİN, Jale

    2014-01-01

    The objective of this study was to compare the performances of calves fed acidified milk replacer (AMR) or sweet (regular) milk replacer (SMR) at 8% of birth weight. Twenty-one calves (10 males, 11 females) were offered replacers reconstituted to 12% of dry matter over 5 weeks. In the group fed AMR, daily body weight gains of calves at the different stages of growth were comparable to those for calves fed SMR. In the preweaning period, calves offered AMR had similar dry matter intakes from st...

  5. HORTALIÇAS ACIDIFICADAS ACIDIFY VEGETABLES

    Directory of Open Access Journals (Sweden)

    Clícia Maria de Jesus BENEVIDES

    1998-08-01

    Full Text Available Objetivou-se no presente trabalho, elaborar um produto à base de hortaliças acidificadas, avaliando suas características. Foram realizadas análises físico-químicas, microbiológicas e o teste de preferência. Pelos resultados apresentados, verificou-se uma variação na acidez total e pH, ausência de contaminação microbiológica e uma aceitabilidade de 78,6% no teste de aceitação após o período de 120 dias à temp. ambiente.The objective in the present work was the preparation of a product " acidify vegetables" evaluating its characteristics. It was made physical-chemical, microbiological analyses and test of preference. From results, it was noticed a variation in the total acidity and pH , absence of microbiological contamination, and an approval of 78,6% in the test of acceptability after the period of 120 days at room temperature.

  6. Diverse coral communities in naturally acidified waters of a Western Pacific reef

    Science.gov (United States)

    Shamberger, Kathryn E. F.; Cohen, Anne L.; Golbuu, Yimnang; McCorkle, Daniel C.; Lentz, Steven J.; Barkley, Hannah C.

    2014-01-01

    Anthropogenic carbon dioxide emissions are acidifying the oceans, reducing the concentration of carbonate ions ([CO32-]) that calcifying organisms need to build and cement coral reefs. To date, studies of a handful of naturally acidified reef systems reveal depauperate communities, sometimes with reduced coral cover and calcification rates, consistent with results of laboratory-based studies. Here we report the existence of highly diverse, coral-dominated reef communities under chronically low pH and aragonite saturation state (Ωar). Biological and hydrographic processes change the chemistry of the seawater moving across the barrier reefs and into Palau's Rock Island bays, where levels of acidification approach those projected for the western tropical Pacific open ocean by 2100. Nevertheless, coral diversity, cover, and calcification rates are maintained across this natural acidification gradient. Identifying the combination of biological and environmental factors that enable these communities to persist could provide important insights into the future of coral reefs under anthropogenic acidification.

  7. Side-band injection of acidified cattle slurry as starter P-fertilization for maize seedlings

    DEFF Research Database (Denmark)

    Petersen, Jens; Lemming, Camilla; Rubæk, Gitte Holton

    Accumulation of phosphorus (P) in agricultural soils has caused increasing environmental concerns. Maize cropped for fodder implies return of animal manures rich in nutrients. In addition, starter fertilization with mineral P is used in cold conditions for maize cropping. It was hypothesized...... that the use of the additional mineral P could be excluded by increased availability of the P applied by animal manures. In a growth chamber experiment we investigated the effect of acidified slurry on the growth and nutrient uptake in maize seedlings. In special designed pot the slurries and mineral reference...

  8. The chemical behavior of acidified chromium (3) solutions. B.S. Thesis

    Science.gov (United States)

    Terman, D. K.

    1981-01-01

    A unique energy-storage system has been developed at NASA's Lewis Research Center called REDOX. This NASA-REDOX system is an electrochemical storage device that utilized the oxidation and reduction of two fully soluble redox couples for charging and discharging. The redox couples now being investigated are acidified chloride solutions of chromium (Cr(+2)/Cr(+3)) and iron (Fe(+2)/Fe(+3)).

  9. Spatially explicit characterization of acidifying and eutrophying air pollution in life-cycle assessment

    NARCIS (Netherlands)

    Huijbregts, Mark A J; Schöpp, Wolfgang; Verkuijlen, Evert; Heijungs, Reinout; Reijnders, Lucas

    2001-01-01

    Simple models are often used to assess the potential impact of acidifying and eutrophying substances released during the life cycle of products. As fate, background depositions, and ecosystem sensitivity are not included in these models, environmental life-cycle assessment of products (LCA) may

  10. Predicting recovery of acidified freshwaters in Europe and Canada: an introduction

    Directory of Open Access Journals (Sweden)

    R. C. Ferrier

    2003-01-01

    Full Text Available Abstract: The RECOVER: 2010 project was designed to assess the current and future anthropogenic pressures on sensitive European freshwater ecosystems. This pan–European assessment utilised a standardised predictive modelling approach to evaluate the degree of compliance with respect to the restoration of acidified waters by 2016, as specified under the EU Water Framework Directive (WFD, and evaluated the environmental benefits of proposed UN-ECE protocols on emissions control. Between 1970 and 2000, observations and model simulations show a significant decline in acidic surface water in all regions of Europe. This demonstrated the success of policies aimed at reducing emission of acidifying compounds. The nature and extent of future regional recovery from acidification is, however, dependent upon the historical pattern of deposition, regional ecosystem characteristics and the role of confounding factors, which may delay the onset of recovery or the magnitude of response. Model predictions to 2010 and beyond emphasise the continued benefit of currently proposed reductions, as reflected by the degree of recovery of freshwater ecosystems. A key component was to link such hydrochemical recovery with ecological response, and the project aimed to evaluate this against current WFD criteria of “good status' and “reference conditions'. The RECOVER: 2010 project research has also played a major role in defining the dynamic modelling outputs which will be required to support the review of the Gothenburg Protocol within the work of the UN-ECE CLRTAP Working Group on Effects (WGE, and model outputs have been made available to a range of national agencies throughout Europe. Keyword: recovery, acidification, modelling, policy, good status, reference conditions

  11. Effects of acetic acid and arginine on pH elevation and growth of Bacillus licheniformis in an acidified cucumber juice medium.

    Science.gov (United States)

    Yang, Zhenquan; Meng, Xia; Breidt, Frederick; Dean, Lisa L; Arritt, Fletcher M

    2015-04-01

    Bacillus licheniformis has been shown to cause pH elevation in tomato products having an initial pH below 4.6 and metabiotic effects that can lead to the growth of pathogenic bacteria. Because of this, the organism poses a potential risk to acidified vegetable products; however, little is known about the growth and metabolism of this organism in these products. To clarify the mechanisms of pH change and growth of B. licheniformis in vegetable broth under acidic conditions, a cucumber juice medium representative of a noninhibitory vegetable broth was used to monitor changes in pH, cell growth, and catabolism of sugars and amino acids. For initial pH values between pH 4.1 to 6.0, pH changes resulted from both fermentation of sugar (lowering pH) and ammonia production (raising pH). An initial pH elevation occurred, with starting pH values of pH 4.1 to 4.9 under both aerobic and anaerobic conditions, and was apparently mediated by the arginine deiminase reaction of B. licheniformis. This initial pH elevation was prevented if 5 mM or greater acetic acid was present in the brine at the same pH. In laboratory media, under favorable conditions for growth, data indicated that growth of the organism was inhibited at pH 4.6 with protonated acetic acid concentrations of 10 to 20 mM, corresponding to 25 to 50 mM total acetic acid; however, growth inhibition required greater than 300 mM citric acid (10-fold excess of the amount in processed tomato products) products under similar conditions. The data indicate that growth and pH increase by B. licheniformis may be inhibited by the acetic acid present in most commercial acidified vegetable products but not by the citric acid in many tomato products.

  12. Thoracic surgery in solving enormous elevation of the left hemidiaphragm

    Directory of Open Access Journals (Sweden)

    Cvijanović Vlado

    2007-01-01

    Full Text Available Background. Acquired elevation of the diaphragm is mostly the result of phrenic nerve paralysis, some of thoracic and abdominal patological states, and also some of neuromuscular diseases. Surgical treatment is rarely performed and is indicated when lung compression produces disabilitating dyspnea, and includes plication of diaphragm. The goal of this case report has been to show completely documented diagnostic procedures and surgical treatment one of rare pathological condition. Case report. A 62-year-old patient was admitted to our clinic because of surgical treatment of the enormous elevation of the left hemidiaphragm. After thoracotomy and plication of the bulging diaphragm, lung compression did not exist any more and mediastinum went back in the normal position. Conclusion. Elevation of the diaphragm rarely demands surgical correction. When it is complicated with lung compression and disabilitating dyspnea, surgical treatment has extremely useful functional effect.

  13. Gastro-oesophageal reflux disease and dental erosions in adults: influence of acidified food intake and impact on quality of life.

    Science.gov (United States)

    Milani, Daiane C; Venturini, Ana Paula C; Callegari-Jacques, Sidia M; Fornari, Fernando

    2016-07-01

    Gastro-oesophageal reflux disease (GORD) and dental erosions (DE) have an established association. We assessed whether GORD is associated with DE controlling for acidified food intake and their relationships with quality of life (QOL). In this cross-sectional study, 419 adult patients who sought dentistry consultation were considered eligible. Patients responded to questionnaires for GORD symptoms, acidified food ingestion and World Health Organization quality of life (WHOQOL Bref), followed by an oral examination, in which DE were characterized according to the Smith & Knight criteria. A total of 417 patients were included (43.8±13.7 years; 68.8% women). There were 143 patients with GORD (34.3%) and 274 controls without GORD. The prevalence of DE was higher in GORD patients compared with the controls (25.9 vs. 17.2%; P=0.041). GORD was associated with DE after adjusting for acidified food intake (P=0.035), with a prevalence ratio of 1.52 (0.95 confidence interval 1.03-2.22). The WHOQOL Bref score was significantly lower in the presence of GORD [median 17.2 (GORD-DE-) vs. 15.4 (GORD+DE+); P<0.01], irrespective of DE. In adults examined in a referential dentistry centre in South America, DE were prevalent and significantly associated with GORD. This association was independent of the intake of acidified food in our study. Impairment in QOL was observed in GORD patients irrespective of the presence of DE.

  14. Acidifier application rate impacts on ammonia emissions from US roaster chicken houses

    Science.gov (United States)

    Shah, Sanjay B.; Grimes, Jesse L.; Oviedo-Rondón, Edgar O.; Westerman, Philip W.

    2014-08-01

    Due to its potential environmental and public health impacts, emissions of ammonia (NH3) as well as several other gases from US livestock farms may be regulated. Broiler houses are important sources of NH3 emissions. However, there are no emissions data from roaster (8-12 wk old broilers, ˜4 kg ea.) houses. Producers treat the litter in broiler houses with acidifiers, such as sodium bisulfate (SBS, NaHSO4) to reduce ammonia production and protect bird health. However, there is very little data on the effect of acidifiers, particularly at high application rates on ammonia emissions. The impact of different SBS application rates [High (0.95-1.46 kg m-2, whole house), Medium (0.73 kg m-2, whole house), Low (0.37-0.49 kg m-2, whole house), and Control (0.37-0.49 kg m-2, brood chamber)] on ammonia emissions was evaluated in commercial roaster houses over 22 months spanning eight flocks. Ammonia emission from each fan was measured with an acid scrubber that operated only when the fan operated. Emissions were calculated using >95% measured data with the rest being estimated using robust methods. Exhaust ammonia-N concentrations were inversely correlated with the SBS application rates. Emission rates on animal unit (AU, where 1 AU = 500 kg live-mass) basis (ER, g d-1 AU-1) were reduced by 27, 13, and 5%, respectively, in the High, Medium, and Low treatments vs. the Control treatment (mean: 100 g d-1 AU-1, range: 86-114 g d-1 AU-1). Emission rates for the Control treatment measured in this study on roasters were mostly higher than ERs in the literature. Differences in ERs are not only due to diet, environmental and management conditions, but also due to measurement methods.

  15. Does road salting confound the recovery of the microcrustacean community in an acidified lake?

    Science.gov (United States)

    Jensen, Thomas Correll; Meland, Sondre; Schartau, Ann Kristin; Walseng, Bjørn

    2014-04-15

    Numerous boreal lakes across the Northern Hemisphere recovering from acidification are experiencing a simultaneous increase in chloride (Cl) concentrations from road salting. Increasing Cl may have profound effects on the lake ecosystem. We examine if an increase in Cl from road salting has modified the recovery of the microcrustacean community in an acidified boreal lake undergoing chemical recovery (study lake). Results from the study lake were compared with an acidified "reference lake". The community changed during the study period in the study lake mainly driven by the reduction in acidification pressure. Despite the community changes and an increase in species richness, the absence of several acid sensitive species, previously occurring in the lake, indicates a delayed biological recovery relative to the chemical recovery. Moreover, changes in occurrence of acid sensitive and acid tolerant species indicated that the biological recovery was slower in the study lake compared to the "reference". Although recurrent episodes of high aluminum and low pH and decreasing Ca are likely important factors for the delay, these do not explain, for instance, the shift from Cyclops scutifer to Bosmina longispina in the study lake. Although the contribution of Cl was not significant, the correlation between Cl and the variation in microcrustacean community was twice as high in the study lake compared to the "reference". We argue that small, sheltered forest lakes may be especially sensitive to increased Cl levels, through changes in pattern of stratification, thus providing a mechanism for the shift from C. scutifer to B. longispina. The reduction of the acidification pressure seems to override the Cl effects on microcrustaceans at low Cl levels in salt-affected lakes recovering from acidification. However, prognoses for growing traffic and increasing road salting raise concern for many recovering lakes located in proximity to roads and urbanized areas. Copyright © 2014

  16. Anaerobic digestion of sulfate-acidified cattle slurry: One-stage vs. two-stage.

    Science.gov (United States)

    Moset, Veronica; Ottosen, Lars Ditlev Mørck; Xavier, Cristiane de Almeida Neves; Møller, Henrik Bjarne

    2016-05-15

    Two strategies to include acidified cattle manure (AcCM) in co-digestion with normal cattle manure (CM) are presented in this work. The strategies are a single thermophilic (50 °C) continuous stirred tank reactor (CSTR) anaerobic digestion and a two-step (65 °C + 50 °C) CSTR process. In both strategies, two different inclusion levels of H2SO4-acidified CM (10% and 20%) in co-digestion with normal CM were tested and compared with a control CSTR fed only CM. Important enhancement of methane (CH4) yield and solid reductions were observed in the thermophilic one-step CSTR working with 10% AcCM. However, a higher inclusion level of AcCM (20%) caused volatile fatty acid accumulation in the reactor and a more than 30% reduction in CH4 production. In terms of CH4 production, when 10% of AcCM was co-digested with 90% of CM, the two-step anaerobic co-digestion yielded less than the single step. During the first step of the two-step CSTR process, acidogenesis and a partial sulfate reduction were achieved. However, sulfide stripping between the first and the second step must be promoted in order to advance this technology. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. Hydrolysis-precipitation studies of aluminum (III) solutions. I. Titration of acidified aluminum nitrate solutions

    NARCIS (Netherlands)

    Vermeulen, A.C.; Geus, John W.; Stol, R.J.; Bruyn, P.L. de

    Acidified aluminum nitrate solutions were titrated with alkali (NaOH or KOH) over a temperature range of 24°C to 90°C. A homogeneous distribution of added base was achieved by: (i) in situ decomposition of urea (90°C); and (ii) a novel method involving injection through a capillary submerged in the

  18. Reduction of hexavalent chromium in water samples acidified for preservation

    Science.gov (United States)

    Stollenwerk, K.G.; Grove, D.B.

    1985-01-01

    Reduction of hexavalent chromium, Cr(VI), in water samples, preserved by standard techniques, was investigated. The standard preservation technique for water samples that are to be analyzed for Cr(VI) consists of filtration through a 0.45-??m membrane, acidification to a pH plastic bottles. Batch experiments were conducted to evaluate the effect of H+ concentration, NO2, temperature, and dissolved organic carbon (DOC) on the reduction of Cr(VI) to Cr(III). The rate of reduction of Cr(VI) to Cr(III) increased with increasing NO2, DOC, H+, and temperature. Reduction of Cr(VI) by organic matter occurred in some samples even though the samples were unacidified. Reduction of Cr(VI) is inhibited to an extent by storing the sample at 4??C. Stability of Cr(VI) in water is variable and depends on the other constituents present in the sample. Water samples collected for the determination of Cr(VI) should be filtered (0.45-??m membrane), refrigerated, and analyzed as quickly as possible. Water samples should not be acidified. Measurement of total Cr in addition to Cr(VI) can serve as a check for Cr(VI) reduction. If total Cr is greater than Cr(VI), the possibility that Cr(VI) reduction has occurred needs to be considered.The rate of reduction of Cr(VI) to Cr(III) increased with increasing NO//2, DOC, H** plus , and temperature. Reduction of Cr(VI) by organic matter occurred in some samples even though the samples were unacidified. Reduction of Cr(VI) is inhibited to an extent by storing the sample at 4 degree C. Stability of Cr(VI) in water is variable and depends on the other constituents present in the sample. Water samples collected for the determination of Cr(VI) should be filtered (0. 45- mu m membrane), refrigerated, and analyzed as quickly as possible. Water samples should not be acidified. Measurement of total Cr in addition to Cr(VI) can serve as a check for Cr(VI) reduction. If total Cr is greater than Cr(VI), the possibility that Cr(VI) reduction has occurred needs

  19. Extraction of steviol glycosides from fresh Stevia using acidified water; clarification followed by ultrafiltration and nanofiltration

    NARCIS (Netherlands)

    Kootstra, A.M.J.; Elissen, H.J.H.; Huurman, Sander

    2016-01-01

    As part of the PPS Kleinschalige bioraffinage project (WP1b), fresh Stevia material was used in the extraction of steviol glycosides using water acidified through conversion of sugar by microorganisms naturally present on the plant. Two successive harvests from the same plot were used. Previous

  20. Nutrient digestibility and mass balance in laying hens fed a commercial or acidifying diet.

    Science.gov (United States)

    Wu-Haan, W; Powers, W J; Angel, C R; Hale, C E; Applegate, T J

    2007-04-01

    The objectives of the current study were to evaluate the effect of an acidifying diet (gypsum) combined with zeolite and slightly reduced crude protein (R) vs. a control diet (C) on nutrient retention in laying hens and compare 3 approaches to estimating nutrient excretion from hens: 1) mass balance calculation (feed nutrients - egg nutrient), 2) use of an indigestible marker with analyzed feed and excreta nutrient content, and 3) an environmental chamber that allowed for capturing all excreted and volatilized nutrients. Hens (n = 640) were allocated randomly to 8 environmental chambers for 3-wk periods. Excreta samples were collected at the end of each trial to estimate apparent retention of N, S, P, and Ca. No diet effects on apparent retention of N were observed (53.44%, P > 0.05). Apparent retention of S, P, and Ca decreased in hens fed R diet (18.7, - 11.4, and 22.6%, respectively) compared with hens fed the C diet (40.7, 0.3, and 28.6%, respectively; P < 0.05). Total N excretion from hens fed the C and R diet was not different (1.16 g/hen/d); however, mass of chamber N remaining in excreta following the 3-wk period was less from hens fed the C diet (1.27 kg) than from hens fed the R diet (1.43 kg). Gaseous emissions of NH(3) over the 3-wk period from hens fed the C diet (0.74 kg per chamber) were greater than emissions from hens fed the R diet (0.45 kg). The 3-wk S excretion mass (estimated using the calculation, indigestible marker, and environmental chamber methods, respectively) was greater from hens fed the R diet (1.85, 1.54, and 1.27 kg, respectively) compared with hens fed the C diet (0.24, 0.20, and 0.14 kg, respectively). The 3-wk P excretion was similar between diets (0.68 kg). Results demonstrate that feeding the acidified diet resulted in decreased N emissions, but because of the acidulant fed, greatly increased S excretion and emissions.

  1. Extraction of steviol glycosides from fresh Stevia using acidified water; comparison to hot water extraction, including purification

    NARCIS (Netherlands)

    Kootstra, A.M.J.; Huurman, Sander

    2017-01-01

    This report describes a practical comparison of an acidified water extraction of freshly harvested Stevia
    plants (the NewFoss method) to the hot water extraction of dried Stevia plants, the industry standard. Both
    extracts are subsequently purified using lab-/bench scale standard industrial

  2. Trends in Surface Water Chemistry in Acidified Areas in Europe and North America from 1990 to 2008

    Science.gov (United States)

    Acidification of lakes and rivers is still an environmental concern despite reduced emissions of acidifying compounds. We analyzed trends in surface water chemistry of 173 acid-sensitive sites from 12 regions in Europe and North America. In 11 of 12 regions, non-marine sulphate (...

  3. Cell specific primary production and phagotrophy of phytoplankton in acidified lakes of Bohemian Forest

    Czech Academy of Sciences Publication Activity Database

    Znachor, Petr; Hrubý, P.

    2000-01-01

    Roč. 4, č. 4 (2000), s. 223-232 ISSN 1211-7420. [Acidified Lakes in the Bohemian/Bavarian Forest - History, Present and Future. České Budějovice, 21.03.2000-23.03.2000] R&D Projects: GA ČR GA206/97/0072; GA ČR GA206/98/0727; GA MŠk FRV:140/1999; GA AV ČR KSK2005601 Subject RIV: DA - Hydrology ; Limnology

  4. African customer is Sun King. Enormous market for solar energy in the Third World

    International Nuclear Information System (INIS)

    Malsch, I.

    2001-01-01

    A brief overview is given in the enormous market for solar energy in Africa. Special attention is paid to one of the leaders in the market for solar energy: Free Energy Europe in Eindhoven, Netherlands. 3 refs

  5. Effects of sulphuric acid and acidifying ammonium deposition on water quality and vegetation of simulated soft water ecosystem

    Energy Technology Data Exchange (ETDEWEB)

    Schuurkes, J.A.A.R.; Heck, I.C.C; Hesen, P.L.G.M.; Leuven, R.S.E.W.; Roelofs, J.G.M.

    1986-11-01

    In a greenhouse, seven identical mini-ecosystems, simulating soft water ponds, were exposed to different types of artificial rain water. The effects of rain water containing H/sub 2/SO/sub 4/ and nitrate, and rain water containing ammonium sulphate on water quality and vegetation were studied and compared. Causal relations were established between rain water quality, water chemistry and changes in floristic composition. Ammonium sulphate deposition, particularly, strongly affected water quality and vegetation development. Although ammonium sulphate deposition was only slightly acid, due to nitrification it acted as an important acid source, causing acidification to pH 3.8. Under acidified conditions, ammonium sulphate deposition led to a luxuriant growth of Juncus bulbosus and Agrostis canina. In the mini-ecosystems, H/sub 2/SO/sub 4/ deposition with a pH of 3.5 only decreased the pH of the water to 5.1 within 1 yr, the acidification of water appeared to be coupled with changes in alkalinity, sulphate, Al, Cd, Ca, Mg, K and inorganic-N. It is concluded that in NH/sub 3/-affected regions in The Netherlands, the high atmospheric deposition of ammonium sulphate probably contributes to a large extent in the acidification, eutrophication and floristic changes of oligotrophic soft waters. 10 references.

  6. Effects of sulphuric acid and acidifying ammonium deposition on water quality and vegetation of simulated soft water ecosystems

    Energy Technology Data Exchange (ETDEWEB)

    Schuurkes, J.A.; Heck, I.C.; Hesen, P.L.; Leuven, R.S.; Roelofs, J.G.

    1986-11-01

    In a greenhouse, seven identical mini-ecosystems, simulating soft water ponds, were exposed to different types of artificial rain water. The effects of rain water containing H/sub 2/SO/sub 4/ and nitrate, and rain water containing ammonium sulfate on water quality and vegetation were studied and compared. Causal relations were established between rain water quality, water chemistry and changes in floristic composition. Ammonium sulfate deposition, particularly, strongly affected water quality and vegetation development. Although ammonium sulfate deposition was only slightly acid, due to nitrification it acted as an important acid source, causing acidification to pH = 3.8. Under acidified conditions, ammonium sulfate deposition lead to a luxuriant growth of Juncus bulbosus and Agrostis canina. In the mini-ecosystems, H/sub 2/SO/sub 4/ deposition with a pH of 3.5 only decreased the pH of the water to 5.1 within 1 yr. The acidification of water appeared to be coupled with changes in alkalinity, sulfate, Al, Cd, Ca, Mg, K and inorganic-N. It is concluded that in NH/sub 3/-affected regions in The Netherlands, the high atmospheric deposition of ammonium sulfate probably contributes to a large extent in the acidification, eutrophication and floristic changes of oligotrophic soft waters. 10 refs.

  7. Acidified nitrite inhibits proliferation of Listeria monocytogenes - Transcriptional analysis of a preservation method.

    Science.gov (United States)

    Müller-Herbst, Stefanie; Wüstner, Stefanie; Kabisch, Jan; Pichner, Rohtraud; Scherer, Siegfried

    2016-06-02

    Sodium nitrite (NaNO2) is added as a preservative during raw meat processing such as raw sausage production to inhibit growth of pathogenic bacteria. In the present study it was shown in challenge assays that the addition of sodium nitrite indeed inhibited growth and survival of Listeria monocytogenes in short-ripened spreadable raw sausages. Furthermore, in vitro growth analyses were performed, which took into account combinations of various parameters of the raw sausage ripening process like temperature, oxygen availability, pH, NaCl concentration, and absence or presence of NaNO2. Data based on 300 growth conditions revealed that the inhibitory effect of nitrite was most prominent in combination with acidification, a combination that is also achieved during short-ripened spreadable raw sausage production. At pH6.0 and below, L. monocytogenes was unable to replicate in the presence of 200mg/l NaNO2. During the adaptation of L. monocytogenes to acidified nitrite stress (pH6.0, 200mg/l NaNO2) in comparison to acid exposure only (pH6.0, 0mg/l NaNO2), a massive transcriptional adaptation was observed using microarray analyses. In total, 202 genes were up-regulated and 204 genes were down-regulated. In accordance with growth inhibition, a down-regulation of genes encoding for proteins which are involved in central cellular processes, like cell wall/membrane/envelope biogenesis, translation and ribosomal structure and biogenesis, transcription, and replication, recombination and repair, was observed. Among the up-regulated genes the most prominent group belonged to poorly characterized genes. A considerable fraction of the up-regulated genes has been shown previously to be up-regulated intracellularly in macrophages, after exposure to acid shock or to be part of the SigB regulon. These data indicate that the adaptation to acidified nitrite partly overlaps with the adaptation to stress conditions being present during host colonization. Copyright © 2016 Elsevier B

  8. Assessment of wood liquefaction in acidified ethylene glycol using experimental design methodology

    Energy Technology Data Exchange (ETDEWEB)

    Rezzoug, S.A. [Universite de La Rochelle, Lab. de Maitrise des Technologies Agro-Industrielles, La Rochelle, 17 (France); Capart, R. [Universite de Technologie de Compiegne, Dept. de Genie Chimique, Compiegne, 60 (France)

    2003-03-01

    The liquefaction of milled wood (Pinus pinaster) was effected in ethylene glycol acidified with small quantities of H{sub 2}SO{sub 4} as catalyst. The purpose of this paper is to evaluate the influence upon the liquefaction yield of the three operating variables, the maximal temperature (150-280 deg C), the reaction time at maximal temperature (20-60 min) and the amount of added H{sub 2}SO{sub 4} (0-1.5% on dry wood). The individual effects, as well as the interactions between operating variables, are investigated by using an experimental design methodology. From a Pareto chart, it appears that the most significant effects are clearly those of the maximum temperature and the interaction between acidity and temperature. Such effects can be graphically verified through response surfaces and contour line plots. From a regression analysis, the conversion rate of wood into liquid is simply expressed as a function of the operating variables by a polynomial containing quadratic terms. A statistical model seems particularly appropriate in the case of complex and multi-components, as wood, a kinetic model is nevertheless proposed for the liquefaction of micro-crystalline cellulose. This model accounts for the formation of a carbonaceous solid residue from the liquid product. Such an unwanted phenomenon obviously results in a lower yield in liquid product. (Author)

  9. Preparation of a new Fenton-like catalyst from red mud using molasses wastewater as partial acidifying agent.

    Science.gov (United States)

    Wei, Guangtao; Shao, Luhua; Mo, Jihua; Li, Zhongmin; Zhang, Linye

    2017-06-01

    Using molasses wastewater as partial acidifying agent, a new Fenton-like catalyst (ACRM sm ) was prepared through a simple process of acidification and calcination using red mud as main material. With molasses wastewater, both the free alkali and the chemically bonded alkali in red mud were effectively removed under the action of H 2 SO 4 and molasses wastewater, and the prepared ACRM sm was a near-neutral catalyst. The ACRM sm preparation conditions were as follows: for 3 g of red mud, 9 mL of 0.7 mol/L H 2 SO 4 plus 2 g of molasses wastewater as the acidifying agent, calcination temperature 573 K, and calcination time 1 h. Iron phase of ACRM sm was mainly α-Fe 2 O 3 and trace amount of carbon existed in ACRM sm . The addition of molasses wastewater not only effectively reduced the consumption of H 2 SO 4 in acidification of red mud but also resulted in the generation of carbon and significantly improved the distribution of macropore in prepared ACRM sm . It was found that near-neutral pH of catalyst, generated carbon, and wide distribution of macropore were the main reasons for the high catalytic activity of ACRM sm . The generated carbon and wide distribution of macropore were entirely due to the molasses wastewater added. In degradation of orange II, ACRM sm retained most of its catalytic stability and activity after five recycling times, indicating ACRM sm had an excellent long-term stability in the Fenton-like process. Furthermore, the performance test of settling showed ACRM sm had an excellent settleability. ACRM sm was a safe and green catalytic material used in Fenton-like oxidation for wastewater treatment.

  10. Interactions between acidified dispersions of milk proteins and dextran or dextran sulfate.

    Science.gov (United States)

    Pachekrepapol, U; Horne, D S; Lucey, J A

    2014-09-01

    Polysaccharides are often used to stabilize cultured milk products, although the nature of these interactions is not entirely clear. The objective of this study was to investigate phase behavior of milk protein dispersions with added dextran (DX; molecular weight = 2 × 10(6) Da) or dextran sulfate (DS; molecular weight = 1.4 × 10(6) Da) as examples of uncharged and charged polysaccharides, respectively. Reconstituted skim milk (5-20% milk solids, wt/wt) was acidified to pH 4.4, 4.6, 4.8, or 4.9 at approximately 0°C (to inhibit gelation) by addition of 3 N HCl. Dextran or DS was added to acidified milk samples to give concentrations of 0 to 2% (wt/wt) and 0 to 1% (wt/wt) polysaccharide, respectively. Milk samples were observed for possible phase separation after storage at 0°C for 1 and 24h. Possible gelation of these systems was determined by using dynamic oscillatory rheology. The type of interactions between caseins and DX or DS was probed by determining the total carbohydrate analysis of supernatants from phase-separated samples. At 5.0 to 7.5% milk solids, phase separation of milk samples occurred after 24h even without DX or DS addition, due to destabilization of caseins in these acidic conditions, and a stabilizing effect was observed when 0.7 or 1.0% DS was added. At higher milk solids content, phase separation was not observed without DX or DS addition. Similar results were observed at all pH levels. Gelation occurred in samples containing high milk solids (≥10%) with the addition of 1.0 to 2.0% DX or 0.4 to 1.0% DS. Based on carbohydrate analysis of supernatants, we believe that DX interacted with milk proteins through a type of depletion flocculation mechanism, whereas DS appeared to interact via electrostatic-type interactions with milk proteins. This study helps to explain how uncharged and charged stabilizers influence the texture of cultured dairy products. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All

  11. Adaptation of chemical methods of analysis to the matrix of pyrite-acidified mining lakes

    International Nuclear Information System (INIS)

    Herzsprung, P.; Friese, K.

    2000-01-01

    Owing to the unusual matrix of pyrite-acidified mining lakes, the analysis of chemical parameters may be difficult. A number of methodological improvements have been developed so far, and a comprehensive validation of methods is envisaged. The adaptation of the available methods to small-volume samples of sediment pore waters and the adaptation of sensitivity to the expected concentration ranges is an important element of the methods applied in analyses of biogeochemical processes in mining lakes [de

  12. Evidence for responses in water chemistry and macroinvertebrates in a strongly acidified mountain stream

    Czech Academy of Sciences Publication Activity Database

    Beneš, F.; Horecký, J.; Senoo, T.; Kamasová, L.; Lamačová, Anna; Tátosová, J.; Hardekopf, D. W.; Stuchlík, Evžen

    2017-01-01

    Roč. 72, č. 9 (2017), s. 1049-1058 ISSN 0006-3088 R&D Projects: GA ČR(CZ) GA17-05935S; GA ČR(CZ) GA15-08124S Institutional support: RVO:86652079 ; RVO:60077344 Keywords : acidified mountain stream * macroinvertebrates * logging * hydrological patterns * recovery Subject RIV: EH - Ecology, Behaviour; EH - Ecology, Behaviour (BC-A) OBOR OECD: Environmental sciences (social aspects to be 5.7); Environmental sciences (social aspects to be 5.7) (BC-A) Impact factor: 0.759, year: 2016

  13. Surrogate analyte approach for quantitation of endogenous NAD(+) in human acidified blood samples using liquid chromatography coupled with electrospray ionization tandem mass spectrometry.

    Science.gov (United States)

    Liu, Liling; Cui, Zhiyi; Deng, Yuzhong; Dean, Brian; Hop, Cornelis E C A; Liang, Xiaorong

    2016-02-01

    A high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) assay for the quantitative determination of NAD(+) in human whole blood using a surrogate analyte approach was developed and validated. Human whole blood was acidified using 0.5N perchloric acid at a ratio of 1:3 (v:v, blood:perchloric acid) during sample collection. 25μL of acidified blood was extracted using a protein precipitation method and the resulting extracts were analyzed using reverse-phase chromatography and positive electrospray ionization mass spectrometry. (13)C5-NAD(+) was used as the surrogate analyte for authentic analyte, NAD(+). The standard curve ranging from 0.250 to 25.0μg/mL in acidified human blood for (13)C5-NAD(+) was fitted to a 1/x(2) weighted linear regression model. The LC-MS/MS response between surrogate analyte and authentic analyte at the same concentration was obtained before and after the batch run. This response factor was not applied when determining the NAD(+) concentration from the (13)C5-NAD(+) standard curve since the percent difference was less than 5%. The precision and accuracy of the LC-MS/MS assay based on the five analytical QC levels were well within the acceptance criteria from both FDA and EMA guidance for bioanalytical method validation. Average extraction recovery of (13)C5-NAD(+) was 94.6% across the curve range. Matrix factor was 0.99 for both high and low QC indicating minimal ion suppression or enhancement. The validated assay was used to measure the baseline level of NAD(+) in 29 male and 21 female human subjects. This assay was also used to study the circadian effect of endogenous level of NAD(+) in 10 human subjects. Copyright © 2015 Elsevier B.V. All rights reserved.

  14. Predictive mapping of the acidifying potential for acid sulfate soils

    DEFF Research Database (Denmark)

    Boman, A; Beucher, Amélie; Mattbäck, S

    Developing methods for the predictive mapping of the potential environmental impact from acid sulfate soils is important because recent studies (e.g. Mattbäck et al., under revision) have shown that the environmental hazards (e.g. leaching of acidity) related to acid sulfate soils vary depending...... on their texture (clay, silt, sand etc.). Moreover, acidity correlates, not only with the sulfur content, but also with the electrical conductivity (EC) measured after incubation. Electromagnetic induction (EMI) data collected from an EM38 proximal sensor also enabled the detailed mapping of acid sulfate soils...... over a field (Huang et al., 2014).This study aims at assessing the use of EMI data for the predictive mapping of the acidifying potential in an acid sulfate soil area in western Finland. Different supervised classification modelling techniques, such as Artificial Neural Networks (Beucher et al., 2015...

  15. Mercury speciation, fluxes, and fate in the volcanically acidified fluids of Copahue volcano, Argentina

    Science.gov (United States)

    Kading, T.; Varekamp, J. C.; Andersson, M.; Balcom, P.; Mason, R. P.

    2010-12-01

    The behavior of mercury in volcanic acid springs and acidified rivers is poorly known, despite the potential impact this vector of contamination has on local surface and ground water quality. Mercury was measured in a volcanically acidified river system (pHvolcano, determined from river water flux measurements and Hg concentrations, was modest and varied between the 3/2008 and 3/2009 sampling campaigns resp. from 0.7 to 1.1 moles/year. The Hg:S ratio of the acid fluids was ~10-8, several orders of magnitude lower than that typically found in volcanic plumes and fumaroles. The small Hg flux and low Hg:S values suggest that the system is either inherently Hg-poor or has lost Hg through vapor loss deeper in the hydrothermal system. Support for the latter comes from high Hg concentrations in geothermal vents and mudpots on the flank of the mountain (24 - 55 ppm Hg). Mercury concentrations decreased conservatively downstream in the river as based on Hg/Cl and Hg/SO4. Non-conservative depletion occurs in the less acidic Lake Caviahue, suggesting that mercury is removed from the water column by sorption to organic matter or other phases. Mercury analyses of a short lake sediment core confirm this (Hg = 0.01 to 0.70 ppm). No evidence was found for preferential uptake of mercury by jarosite, schwertmannite, or goethite, although the latter two phases precipitate in the most distal and Hg-depleted section of the fluvial system.

  16. Trichodesmium’s strategies to alleviate phosphorus limitation in the future acidified oceans.

    Science.gov (United States)

    Spungin, Dina; Berman-Frank, Ilana; Levitan, Orly

    2014-06-01

    Global warming may exacerbate inorganic nutrient limitation, including phosphorus (P), in the surface waters of tropical oceans that are home to extensive blooms of the marine diazotrophic cyanobacterium, Trichodesmium. We examined the combined effects of P limitation and pCO(2), forecast under ocean acidification scenarios, on Trichodesmium erythraeum IMS101 cultures. We measured nitrogen acquisition,glutamine synthetase activity, C uptake rates, intracellular Adenosine Triphosphate (ATP) concentration and the pool sizes of related key proteins. Here, we present data supporting the idea that cellular energy re-allocation enables the higher growth and N(2) fixation rates detected in Trichodesmium cultured under high pCO(2). This is reflected in altered protein abundance and metabolic pools. Also modified are particulate organic carbon and nitrogen production rates,enzymatic activities, and cellular ATP concentrations. We suggest that adjusting these cellular pathways to changing environmental conditions enables Trichodesmium to compensate for low P availability and to thrive in acidified oceans. Moreover, elevated pCO(2) could provide Trichodesmium with a competitive dominance that would extend its niche, particularly in P-limited regions of the tropical and subtropical oceans.

  17. European transboundary acidifying air pollution. Ten years calculated fields and budgets to the end of the first Sulphur Protocol

    Energy Technology Data Exchange (ETDEWEB)

    Barrett, K.; Seland, Oe.; Foss, A.; Mylona, S.; Sandnes, H.; Styve, H.; Tarrason, L.

    1995-07-01

    The Cooperative Programme for the Monitoring and Evaluation of the Long Range Transmission and Air Pollutants in Europe, EMEP, plays an integral part in data collection and scientific cooperation for implementation of the 1979 Geneva Convention on Long Range Transboundary Air Pollution. Within EMEP, the Meteorological Synthesizing Centre - West (MSC-W) is an international technical centre. The purpose of the MSC-W, focusing in part on acidifying substances, is to estimate the concentrations of relevant sulphur and nitrogen pollutants across Europe on the basis of emission information and meteorological data, and to estimate the transboundary fluxes of these substances. Responding to these specific obligations, the report presents calculations of sulphur and nitrogen concentrations and depositions and of their transboundary fluxes. The calculations are performed by the receptor oriented one layer trajectory (Lagrangian) acid deposition model, which during 1995 has been used to estimate acidifying pollutant fluxes for the ten year period 1985-1994. This corresponds to the period between initial signing and conclusion of the first Sulphur Protocol, signed in Helsinki in 1985. 90 refs., 42 figs., 43 tabs.

  18. Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades

    Directory of Open Access Journals (Sweden)

    Masako Nakamura

    2012-02-01

    Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsps and hsf1 by quantitative real-time polymerase chain reaction (PCR. Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO2 conditions. These findings indicate that the expression of hsps is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression.

  19. Effects of watershed and in-stream liming on macroinvertebrate communities in acidified tributaries to an Adirondack lake

    Science.gov (United States)

    George, Scott D.; Baldigo, Barry P.; Lawrence, Gregory B.; Fuller, Randall L.

    2018-01-01

    Liming techniques are being explored as a means to accelerate the recovery of aquatic biota from decades of acid deposition in many regions. The preservation or restoration of native sportfish populations has typically been the impetus for liming programs, and as such, less attention has been given to its effects on other biological assemblages such as macroinvertebrates. Furthermore, the differing effects of various lime application strategies such as in-stream and watershed applications are not well understood. In 2012, a program was initiated using in-stream and aerial (whole-watershed) liming to improve water quality and Brook Trout (Salvelinus fontinalis) recruitment in three acidified tributaries of a high-elevation Adirondack lake in New York State. Concurrently, macroinvertebrates were sampled annually between 2013 and 2016 at 3 treated sites and 3 untreated reference sites to assess the effects of each liming technique on this community. Despite improvements in water chemistry in all three limed streams, our results generally suggest that neither liming technique succeeded in improving the condition of macroinvertebrate communities. The watershed application caused an immediate and unsustained decrease in the density of macroinvertebrates and increase in the proportion of sensitive taxa. These changes were driven primarily by a one-year 71 percent reduction of the acid-tolerant Leuctra stoneflies and likely represent an initial chemistry shock from the lime application rather than a recovery response. The in-stream applications appeared to reduce the density of macroinvertebrates, particularly in one stream where undissolved lime covered the natural substrate. The close proximity of our study sites to the in-stream application points (50 and 1230 m) may partly explain these negative effects. Our results are consistent with prior studies of in-stream liming which indicate that this technique often fails to restore macroinvertebrate communities to a pre

  20. Standard test method for evaluating stress-corrosion cracking of stainless alloys with different nickel content in boiling acidified sodium chloride solution

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2000-01-01

    1.1 This test method describes a procedure for conducting stress-corrosion cracking tests in an acidified boiling sodium chloride solution. This test method is performed in 25% (by mass ) sodium chloride acidified to pH 1.5 with phosphoric acid. This test method is concerned primarily with the test solution and glassware, although a specific style of U-bend test specimen is suggested. 1.2 This test method is designed to provide better correlation with chemical process industry experience for stainless steels than the more severe boiling magnesium chloride test of Practice G36. Some stainless steels which have provided satisfactory service in many environments readily crack in Practice G36, but have not cracked during interlaboratory testing using this sodium chloride test method. 1.3 This boiling sodium chloride test method was used in an interlaboratory test program to evaluate wrought stainless steels, including duplex (ferrite-austenite) stainless and an alloy with up to about 33% nickel. It may also b...

  1. Survival of Escherichia coli O157:H7 in synthetic gastric fluid after cold and acid habituation in apple juice or trypticase soy broth acidified with hydrochloric acid or organic acids.

    Science.gov (United States)

    Uljas, H E; Ingham, S C

    1998-08-01

    Extreme acid tolerance of Escherichia coli O157:H7 has raised doubts about the safety of acidic foods. This study examined whether prior storage in acidic and/or cold conditions enhanced survival of E. coli O157:H7 in synthetic gastric fluid (SGF). Three E. coli O157:H7 strains were stored in trypticase soy broth (TSB; acidified with HCl, malic acid, citric acid, or lactic acid) or pH 3.5 and 6.5 (nonacidic control) apple juice at 4 and 21 degrees C for acids, suggesting that juice constituents other than organic acids protect E. coli O157:H7. Refrigeration combined with low pH best protected cells in apple juice and acidified TSB, but, compared to the nonacidic control, only acidified TSB enhanced subsequent survival in pH 2.5 SGF. Equal survival in SGF occurred after storage in pH 3.5 or 6.5 apple juice at 4 degrees C, suggesting that low temperature alone in apple juice enhanced acid tolerance. Two strains stored at 4 degrees C in TSB containing malic or citric acid subsequently survived better in SGF than cells stored in nonacidified TSB but poorer than cells stored in the presence of HCl. These differences reflect the higher pKa of these organic acids. However, subsequent survival of these strains in SGF was poorer after refrigerated storage in apple juice than in TSB containing citric or malic acids. Cells stored in lactic acid were most likely to be completely eliminated upon transfer to SGF. Differences in survival in storage media or SGF related to strain, storage conditions, or acidifier were consistent and often statistically significant (P acidic beverages may not be affected by the type of acidifier used, the subsequent survival in SGF of this pathogen may be critically dependent on this factor.

  2. Corrosion of Alloys 600 ampersand 900 in acidified sulfate and chloride solutions

    International Nuclear Information System (INIS)

    Cullen, W.H.; Partridge, M.J.; Paine, J.P.N.

    1993-01-01

    A program is being performed currently: (1) to better quantify the susceptibility of Alloy 600 and 690 steam generator (SG) tubing materials to intergranular attack (IGA) and intergranular stress corrosion cracking (IGSCC) in acid solutions of the types that could concentrate in steam generator crevices, and (2) to establish the effectiveness of various remedial measures achieved through chemical additions to the secondary side coolant. The main test method is the exposure of stressed C-rings and expanded capsules of SG tubing to acid chloride and sulfate environments of various pH levels, temperatures, and applied potentials. Following these exposures, crack lengths are measured on metallographic cross-sections of the C-rings, and wastage rates calculated from weight loss, surface area and time. Test solutions are based on varying concentrations of sulfate and chloride species, with other contaminants added to some tests. The temperature dependence of the pH of the acidified solutions is calculated using the EPRI-developed MULTEQ computer program. High-temperature pH levels range from values of 2.5 to 7.6 at 315 degrees C, which is the nominal test temperature. Various accelerating and inhibiting factors (pH, temperature, solution composition and concentration, and applied electrochemical potential) are being evaluated

  3. Inhibition of Listeria monocytogenes on cooked cured chicken breasts by acidified coating containing allyl isothiocyanate or deodorized Oriental mustard extract.

    Science.gov (United States)

    Olaimat, Amin N; Holley, Richard A

    2016-08-01

    Ready-to-eat meats are considered foods at high risk to cause life-threatening Listeria monocytogenes infections. This study screened 5 L. monocytogenes strains for their ability to hydrolyze sinigrin (a glucosinolate in Oriental mustard), which formed allyl isothiocyanate (AITC) and reduced L. monocytogenes viability on inoculated vacuum-packed, cooked, cured roast chicken slices at 4 °C. Tests involved incorporation of 25-50 μl/g AITC directly or 100-250 mg/g Oriental mustard extract in 0.5% (w/v) κ-carrageenan/2% (w/v) chitosan-based coatings prepared using 1.5% malic or acetic acid. L. monocytogenes strains hydrolyzed 33.6%-48.4% pure sinigrin in MH broth by 21 d at 25 °C. Acidified κ-carrageenan/chitosan coatings containing 25-50 μl/g AITC or 100-250 mg/g mustard reduced the viability of L. monocytogenes and aerobic bacteria on cooked, cured roast chicken slices by 4.1 to >7.0 log10 CFU/g compared to uncoated chicken stored at 4 °C for 70 d. Coatings containing malic acid were significantly more antimicrobial than those with acetic acid. During storage for 70 d, acidified κ-carrageenan/chitosan coatings containing 25-50 μl/g AITC or 250 mg/g mustard extract reduced lactic acid bacteria (LAB) numbers 3.8 to 5.4 log10 CFU/g on chicken slices compared to uncoated samples. Acidified κ-carrageenan/chitosan-based coatings containing either AITC or Oriental mustard extract at the concentrations tested had the ability to control L. monocytogenes viability and delay growth of potential spoilage bacteria on refrigerated, vacuum-packed cured roast chicken. Copyright © 2016. Published by Elsevier Ltd.

  4. Not an Oxymoron: Some X-ray Binary Pulsars with Enormous Spinup Rates Reveal Weak Magnetic Fields

    Science.gov (United States)

    Christodoulou, D. M.; Laycock, S. G. T.; Kazanas, D.

    2018-05-01

    Three high-mass X-ray binaries have been discovered recently exhibiting enormous spinup rates. Conventional accretion theory predicts extremely high surface dipolar magnetic fields that we believe are unphysical. Instead, we propose quite the opposite scenario: some of these pulsars exhibit weak magnetic fields, so much so that their magnetospheres are crushed by the weight of inflowing matter. The enormous spinup rate is achieved before inflowing matter reaches the pulsar's surface as the penetrating inner disk transfers its excess angular momentum to the receding magnetosphere which, in turn, applies a powerful spinup torque to the pulsar. This mechanism also works in reverse: it spins a pulsar down when the magnetosphere expands beyond corotation and finds itself rotating faster than the accretion disk which then exerts a powerful retarding torque to the magnetic field and to the pulsar itself. The above scenaria cannot be accommodated within the context of neutron-star accretion processes occurring near spin equilibrium, thus they constitute a step toward a new theory of extreme (far from equilibrium) accretion phenomena.

  5. Preservation of acidified cucumbers with a natural preservative combination of fumaric acid and allyl isothiocyanate that target lactic acid bacteria and yeasts

    Science.gov (United States)

    Without the addition of preservative compounds cucumbers acidified with 150 mM acetic acid with pH adjusted to 3.5 typically undergo fermentation by lactic acid bacteria. Fumaric acid (20 mM) inhibited growth of Lactobacillus plantarum and the lactic acid bacteria present on fresh cucumbers, but sp...

  6. Recovery of acidified mountain lakes in Norway as predicted by the MAGIC model

    Directory of Open Access Journals (Sweden)

    Bernard J. COSBY

    2004-02-01

    Full Text Available As part of the EU project EMERGE the biogeochemical model MAGIC was used to reconstruct acidification history and predict future recovery for mountain lakes in two regions of Norway. Central Norway (19 lakes receives low levels of acid deposition, most of the lakes have undergone only minor amounts of acidification, and all are predicted to recover in the future. Central Norway thus represents a reference area for more polluted regions in southern Norway and elsewhere in Europe. Southern Norway (23 lakes, on the other hand, receives higher levels of acid deposition, nearly all the studied lakes were acidified and had lost fish populations, and although some recovery has occurred during the period 1980-2000 and additional recovery is predicted for the next decades, the model simulations indicated that the majority of the lakes will not achieve water quality sufficient to support trout populations. Uncertainties in these predictions include possible future N saturation and the exacerbating effects of climate change. The mountain lakes of southern Norway are among the most sensitive in Europe. For southern Norway additional measures such as stricter controls of emissions of air pollutants will be required to obtain satisfactory water quality in the future.

  7. Similar Data Retrieval from Enormous Datasets on ELF/VLF Wave Spectrum Observed by Akebono

    Directory of Open Access Journals (Sweden)

    Y Kasahara

    2010-02-01

    Full Text Available As the total amount of data measured by scientific spacecraft is drastically increasing, it is necessary for researchers to develop new computation methods for efficient analysis of these enormous datasets. In the present study, we propose a new algorithm for similar data retrieval. We first discuss key descriptors that represent characteristics of the VLF/ELF waves observed by the Akebono spacecraft. Second, an algorithm for similar data retrieval is introduced. Finally, we demonstrate that the developed algorithm works well for the retrieval of the VLF spectrum with a small amount of CPU load.

  8. The Effects of Ocean Acidification on Predator-Prey Interactions between Mya arenaria and Callinectes sapidus

    Science.gov (United States)

    Longmire, K.; Glaspie, C.; Seitz, R.

    2016-02-01

    The study examined the implications of ocean acidification for Mya arenaria and the predator-prey dynamics between M. arenaria and Callinectes sapidus. Clams were subjected to either ambient conditions or acidified conditions and grown over four weeks. Mortality, shell lengths, and biomass (ash-free dry weights) were recorded for clams destructively sampled each week. Clams were subjected to behavioral experiments to determine their response to an approaching physical disturbance. Crabs were exposed to acidified or ambient conditions for 48 hours, and placed in 48 hour mesocosm trials with clams. Shell lengths, mortality and biomass between the ambient and acidified clams were not significantly different between acidified and ambient treatments. Shell ash weights were lower for acidified clams, evidence of shell dissolution. In the behavioral experiment, ocean acidification reduced the ability of clams to respond to a predator stimulus. Lastly, in predator-prey mesocosm trials, in ambient conditions, crabs ate all or none of the available clams, whereas acidified crabs ate all available clams in many trials and ate at least one acidified clam per trial. The early effects of ocean acidification on M. arenaria will manifest in trophic interactions with other species, rather than impacting M. arenaria alone.

  9. Nitrite addition to acidified sludge significantly improves digestibility, toxic metal removal, dewaterability and pathogen reduction

    Science.gov (United States)

    Du, Fangzhou; Keller, Jürg; Yuan, Zhiguo; Batstone, Damien J.; Freguia, Stefano; Pikaar, Ilje

    2016-12-01

    Sludge management is a major issue for water utilities globally. Poor digestibility and dewaterability are the main factors determining the cost for sludge management, whereas pathogen and toxic metal concentrations limit beneficial reuse. In this study, the effects of low level nitrite addition to acidified sludge to simultaneously enhance digestibility, toxic metal removal, dewaterability and pathogen reduction were investigated. Waste activated sludge (WAS) from a full-scale waste water treatment plant was treated at pH 2 with 10 mg NO2--N/L for 5 h. Biochemical methane potential tests showed an increase in the methane production of 28%, corresponding to an improvement from 247 ± 8 L CH4/kg VS to 317 ± 1 L CH4/kg VS. The enhanced removal of toxic metals further increased the methane production by another 18% to 360 ± 6 L CH4/kg VS (a total increase of 46%). The solids content of dewatered sludge increased from 14.6 ± 1.4% in the control to 18.2 ± 0.8%. A 4-log reduction for both total coliforms and E. coli was achieved. Overall, this study highlights the potential of acidification with low level nitrite addition as an effective and simple method achieving multiple improvements in terms of sludge management.

  10. Comparison of Spatiotemporal Mapping Techniques for Enormous Etl and Exploitation Patterns

    Science.gov (United States)

    Deiotte, R.; La Valley, R.

    2017-10-01

    The need to extract, transform, and exploit enormous volumes of spatiotemporal data has exploded with the rise of social media, advanced military sensors, wearables, automotive tracking, etc. However, current methods of spatiotemporal encoding and exploitation simultaneously limit the use of that information and increase computing complexity. Current spatiotemporal encoding methods from Niemeyer and Usher rely on a Z-order space filling curve, a relative of Peano's 1890 space filling curve, for spatial hashing and interleaving temporal hashes to generate a spatiotemporal encoding. However, there exist other space-filling curves, and that provide different manifold coverings that could promote better hashing techniques for spatial data and have the potential to map spatiotemporal data without interleaving. The concatenation of Niemeyer's and Usher's techniques provide a highly efficient space-time index. However, other methods have advantages and disadvantages regarding computational cost, efficiency, and utility. This paper explores the several methods using a range of sizes of data sets from 1K to 10M observations and provides a comparison of the methods.

  11. COMPARISON OF SPATIOTEMPORAL MAPPING TECHNIQUES FOR ENORMOUS ETL AND EXPLOITATION PATTERNS

    Directory of Open Access Journals (Sweden)

    R. Deiotte

    2017-10-01

    Full Text Available The need to extract, transform, and exploit enormous volumes of spatiotemporal data has exploded with the rise of social media, advanced military sensors, wearables, automotive tracking, etc. However, current methods of spatiotemporal encoding and exploitation simultaneously limit the use of that information and increase computing complexity. Current spatiotemporal encoding methods from Niemeyer and Usher rely on a Z-order space filling curve, a relative of Peano’s 1890 space filling curve, for spatial hashing and interleaving temporal hashes to generate a spatiotemporal encoding. However, there exist other space-filling curves, and that provide different manifold coverings that could promote better hashing techniques for spatial data and have the potential to map spatiotemporal data without interleaving. The concatenation of Niemeyer’s and Usher’s techniques provide a highly efficient space-time index. However, other methods have advantages and disadvantages regarding computational cost, efficiency, and utility. This paper explores the several methods using a range of sizes of data sets from 1K to 10M observations and provides a comparison of the methods.

  12. Food selectivity of perch (Perca fluviatilis L. ) in acidified lakes on the west coast of Sweden

    Energy Technology Data Exchange (ETDEWEB)

    Andersson, B

    1972-01-01

    The objective of the present paper was to examine presumed changes in the food composition of perch in lakes subjected to recent acidification, which emanates from sulfuric acid in the precipitation. The material analyzed (825 preserved specimens from 49 different lakes) was collected by the Fishery Board. These lakes represent a great variability in terms of pH. The perch were divided into four size groups and the stomach contents of each specimen was analyzed qualitatively and quantitatively. The results indicate that fish, which make up the bulk of food for adult perch, are replaced by various invertebrates at a decreasing pH. With a moderate decrease the water hog-louse (Asellus aquaticus) appears of major importance, whereas its place is taken by Corixa bugs in the most acidified lakes. These latter invertebrates are normally absent in the food of adult perch. Also, plankton accounts for a substantial share of the stomach contents. The reasons for these changes are manifold, but two factors are primarily discussed in this paper, viz. the effect of the acidification on the prey populations and the elimination of certain species by selective predation in a normal lake.

  13. Emissions of acidifying air pollutants in the North West region of England

    Energy Technology Data Exchange (ETDEWEB)

    Longhurst, J.W.S.; Lindley, S.J.; Conlan, D.E. [Manchester Metropolitan University, Manchester (United Kingdom). Dept. of Environmental and Geographical Sciences

    1995-12-01

    Most estimates of emission are concerned with the nation state level. This paper discusses methods utilised in the estimates of emissions to the atmosphere of sulphur dioxide, volatile organic compounds and oxides of nitrogen from a densely populated and heavily industrialised region of the United Kingdom. Data on power generation from coal, industrial plant, fuel usage, air, sea and road transportation, and human population statistics have been integrated into a method to provide regional emission estimates. The resulting emission patterns are described in terms of sources and emission density. Spatial and temporal patterns are identified and major sources of emissions discussed in terms of national control programmes. Transportation is the dominant source of oxides of nitrogen emissions whilst power generation is the dominant source of sulphur dioxide. The relative importance of the North West as an emission source within the UK is assessed. The change in the strengths of acidifying emissions between 1987 and 1992 is discussed and the rate of change in emission magnitudes between the North West region and the UK as a whole compared. 9 refs., 4 tabs.

  14. Emissions of acidifying air pollutants in the North West region of England

    International Nuclear Information System (INIS)

    Longhurst, J.W.S.; Lindley, S.J.; Conlan, D.E.

    1995-01-01

    Most estimates of emission are concerned with the nation state level. This paper discusses methods utilised in the estimates of emissions to the atmosphere of sulphur dioxide, volatile organic compounds and oxides of nitrogen from a densely populated and heavily industrialised region of the United Kingdom. Data on power generation from coal, industrial plant, fuel usage, air, sea and road transportation, and human population statistics have been integrated into a method to provide regional emission estimates. The resulting emission patterns are described in terms of sources and emission density. Spatial and temporal patterns are identified and major sources of emissions discussed in terms of national control programmes. Transportation is the dominant source of oxides of nitrogen emissions whilst power generation is the dominant source of sulphur dioxide. The relative importance of the North West as an emission source within the UK is assessed. The change in the strengths of acidifying emissions between 1987 and 1992 is discussed and the rate of change in emission magnitudes between the North West region and the UK as a whole compared. 9 refs., 4 tabs

  15. Neonates with Bartter syndrome have enormous fluid and sodium requirements.

    Science.gov (United States)

    Azzi, Antonio; Chehade, Hassib; Deschênes, Georges

    2015-07-01

    Managing neonatal Bartter syndrome by achieving adequate weight gain is challenging. We assessed the correlation between weight gain in neonatal Bartter syndrome and the introduction of fluid and sodium supplementations and indomethacin during the first 4 weeks of life. Daily fluid and electrolytes requirements were analysed using linear regression and Spearman correlation coefficients. The weight gain coefficient was calculated as daily weight gain after physiological neonatal weight loss. We studied seven infants. The highest weight gain coefficients occurred between weeks two and four in the five neonates who either received prompt amounts of fluid (maximum 810 mL/kg/day) and sodium (maximum 70 mmol/kg/day) or were treated with indomethacin. For the two patients with the highest weight gain coefficient, water and sodium supplementations were decreased in weeks two to four leading to a significant negative Spearman correlation between weight gain and fluid supplements (r = -0.55 and -0.68) and weight gain and sodium supplementations (r = -0.96 and -0.72). The two patients with the lowest weight gain coefficient had positive Spearman correlation coefficients between weight gain and fluid and sodium supplementations. Infants with neonatal Bartter syndrome required rapid and enormous fluid and sodium supplementations or the early introduction of indomethacin treatment to achieve adequate weight gain during the early postnatal period. ©2015 Foundation Acta Paediatrica. Published by John Wiley & Sons Ltd.

  16. Modelling impacts of temperature, and acidifying and eutrophying deposition on DOC trends

    Science.gov (United States)

    Sawicka, Kasia; Rowe, Ed; Evans, Chris; Monteith, Don; Vanguelova, Elena; Wade, Andrew; Clark, Joanna

    2017-04-01

    Surface water dissolved organic carbon (DOC) concentrations in large parts of the northern hemisphere have risen over the past three decades, raising concern about enhanced contributions of carbon to the atmosphere and seas and oceans. The effect of declining acid deposition has been identified as a key control on DOC trends in soil and surface waters, since pH and ionic strength affect sorption and desorption of DOC. However, since DOC is derived mainly from recently-fixed carbon, and organic matter decomposition rates are considered sensitive to temperature, uncertainty persists regarding the extent to the relative importance of different drivers that affect these upward trends. We ran the dynamic model MADOC (Model of Acidity and Soil Organic Carbon) for a range of UK soils (podzols, gleysols and peatland), for which the time-series were available, to consider the likely relative importance of decreased deposition of sulphate and chloride, accumulation of reactive N, and higher temperatures, on DOC production in different soils. Modelled patterns of DOC change generally agreed favourably with measurements collated over 10-20 years, but differed markedly between sites. While the acidifying effect of sulphur deposition appeared to be the predominant control on the observed soil water DOC trends in all the soils considered other than a blanket peat, the model suggested that over the long term, the effects of nitrogen deposition on N-limited soils may have been sufficient to elevate the DOC recovery trajectory significantly. The second most influential cause of rising DOC in the model simulations was N deposition in ecosystems that are N-limited and respond with stimulated plant growth. Although non-marine chloride deposition made some contribution to acidification and recovery, it was not amongst the main drivers of DOC change. Warming had almost no effect on modelled historic DOC trends, but may prove to be a significant driver of DOC in future via its influence

  17. Two-phase anaerobic digestion of partially acidified sewage sludge: a pilot plant study for safe sludge disposal in developing countries.

    Science.gov (United States)

    Passio, Luca; Rizzoa, Luigi; Fuchs, Stephan

    2012-09-01

    The unsafe disposal of wastewater and sludge in different areas of developing countries results in significant environmental pollution, particularly for groundwater, thus increasing the risk of waterborne diseases spreading. In this work, a two-phase anaerobic digestion process for post-treatment of partially acidified sewage sludge was investigated to evaluate its feasibility as a safe sludge disposal system. Pilot tests showed that an effective sludge stabilization can be achieved (total volatile solids content <65%, organic acid concentration <200 mg/L at flow rate = 50 L/d and hydraulic residence time = 18 d) as well as a relative low faecal coliform density (<1000 most probable number per g total solids), showing that land application of the sludge without restrictions is possible according to US Environmental Protection Agency criteria for safe sludge disposal. A biogas production as high as 390 L/d with a 60% methane content by volume was achieved, showing that energy production from biogas may be achieved as well.

  18. Application of Impedance Microbiology for Evaluating Potential Acidifying Performances of Starter Lactic Acid Bacteria to Employ in Milk Transformation.

    Science.gov (United States)

    Bancalari, Elena; Bernini, Valentina; Bottari, Benedetta; Neviani, Erasmo; Gatti, Monica

    2016-01-01

    Impedance microbiology is a method that enables tracing microbial growth by measuring the change in the electrical conductivity. Different systems, able to perform this measurement, are available in commerce and are commonly used for food control analysis by mean of measuring a point of the impedance curve, defined "time of detection." With this work we wanted to find an objective way to interpret the metabolic significance of impedance curves and propose it as a valid approach to evaluate the potential acidifying performances of starter lactic acid bacteria to be employed in milk transformation. To do this it was firstly investigated the possibility to use the Gompertz equation to describe the data coming from the impedance curve obtained by mean of BacTrac 4300®. Lag time (λ), maximum specific M% rate (μmax), and maximum value of M% (Yend) have been calculated and, given the similarity of the impedance fitted curve to the bacterial growth curve, their meaning has been interpreted. Potential acidifying performances of eighty strains belonging to Lactobacillus helveticus, Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis , and Streptococcus thermophilus species have been evaluated by using the kinetics parameters, obtained from Excel add-in DMFit version 2.1. The novelty and importance of our findings, obtained by means of BacTrac 4300®, is that they can also be applied to data obtained from other devices. Moreover, the meaning of λ, μmax, and Yend that we have extrapolated from Modified Gompertz equation and discussed for lactic acid bacteria in milk, can be exploited also to other food environment or other bacteria, assuming that they can give a curve and that curve is properly fitted with Gompertz equation.

  19. Trends in emissions of acidifying species in Asia, 1985-1997

    International Nuclear Information System (INIS)

    Streets, D. G.; Tsai, N. Y.; Akimoto, H.; Oka, K.

    2000-01-01

    Acid deposition is a serious problem throughout much of Asia. Emissions of sulfur dioxide (SO 2 ) and nitrogen oxides (NO x ) have been increasing steadily, as nations strive to increase their levels of economic development. Coal and fuel oil have been the main choices for powering industrial development; and, until recently, only a few countries (notably Japan and Taiwan) had taken significant steps to avert the atmospheric emissions that accompany fuel combustion. This paper discusses trends in emissions of SO 2 and NO x that have occurred in Asian countries in the period 1985--1997, using results from the RAINS-ASIA computer model and energy-use trends from the IEA Energy Statistics and Balances database. Emissions of SO 2 in Asia grew from 26.6 Tg in 1985 to 33.7 Tg in 1990 and to 39.2 Tg in 1997. Though SO 2 emissions used to grow as fast as fossil-fuel use, recent limitations on the sulfur content of coal and oil have slowed the growth. The annual-average emissions growth between 1990 and 1997 was only 1.1%, considerably less than the economic growth rate. Emissions of NO x , on the other hand, continue to grow rapidly, from 14.1 Tg in 1985 to 18.7 Tg in 1990 and 28.5 Tg in 1997, with no signs of abating. Thus, though SO 2 remains the major contributor to acidifying emissions in Asia, the role of NO x , will become more and more important in the future

  20. Increased microsporidian parasitism of the crayfish Orconectes virilis in an experimentally acidified lake. [Thelohania contejeani

    Energy Technology Data Exchange (ETDEWEB)

    France, R.L.; Graham, L.

    1985-10-01

    Orconectes virilis has been identified as a host of the microsporidian Thelohania contejeani in four lakes in the Experimental Lakes Area (ELA), northwestern Ontario. Prevalence of parasitism increased from 1.7% in autumn 1979, to 6.5% and 7.7% in autumns 1980 and 1981 concomitant with experimental acidification of Lake 223 to pH 5.1. By comparison, mean infection rates observed within three reference lakes were between 0.3 to 0.6%. Enhanced microsporidosis is believed to have contributed to a reduction in annual crayfish survival rates for the Lake 223 population of 8% in 1979 and 18% in 1980 compared to averages calculated for the non-acidified lakes. Hypotheses to explain the ten-fold increase in Thelohania parasitism in Lake 223 over background levels for the ELA region include a) increased probagative ability due to elevated rate of crayfish cannibalism or low pH-favored parasite life cycle, and b) decreased host resistance when under sublethal stress.

  1. Rapid restoration of methanogenesis in an acidified UASB reactor treating 2,4,6-trichlorophenol (TCP).

    Science.gov (United States)

    Díaz-Báez, María Consuelo; Valderrama-Rincon, Juan Daniel

    2017-02-15

    Anaerobic bioreactors are often used for removal of xenobiotic and highly toxic pollutants from wastewater. Most of the time, the pollutant is so toxic that the stability of the reactor becomes compromised. It is well known that methanogens are one of the most sensitive organisms in the anaerobic consortia and hence the stability of the reactors is highly dependant on methanogenesis. Unfortunately few studies have focused on recovering the methanogenic activity once it has been inhibited by highly toxic pollutants. Here we establish a quick recovery strategy for neutralization of an acidified UASB reactor after failure by intoxication with an excess of TCP in the influent. Once the reactor returned to pH values compatible with methanogenesis, biogas production was re-started after one day and the system was re-acclimated to TCP. Successful removal of TCP from synthetic wastewater was shown for concentrations up to 70mg/L after restoration. Copyright © 2016 Elsevier B.V. All rights reserved.

  2. Application of impedance microbiology for evaluating potential acidifying performances of starter lactic acid bacteria to employ in milk transformation

    Directory of Open Access Journals (Sweden)

    Elena Bancalari

    2016-10-01

    Full Text Available Impedance microbiology is a method that enables tracing microbial growth by measuring the change in the electrical conductivity. Different systems, able to perform this measurement, are available in commerce and are commonly used for food control analysis by mean of measuring a point of the impedance curve, defined time of detection. With this work we wanted to find an objective way to interpret the metabolic significance of impedance curves and propose it as a valid approach to evaluate the potential acidifying performances of starter lactic acid bacteria to be employed in milk transformation. To do this it was firstly investigated the possibility to use the Gompertz equation to describe the data coming from the impedance curve obtained by mean of BacTrac 4300®. Lag time (λ, maximum specific M% rate (µmax, and maximum value of M% (Yend have been calculated and, given the similarity of the impedance fitted curve to the bacterial growth curve, their meaning has been interpreted. Potential acidifying performances of eighty strains belonging to Lactobacillus helveticus, Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis and Streptococcus thermophilus species have been evaluated by using the kinetics parameters, obtained from Excel add-in DMFit version 2.1. The novelty and importance of our findings, obtained by means of BacTrac 4300®, is that they can also be applied to data obtained from other devices. Moreover, the meaning of λ, μmax and Yend that we have extrapolated from Modified Gompertz equation and discussed for lactic acid bacteria in milk, can be exploited also to other food environment or other bacteria, assuming that they can give a curve and that curve is properly fitted with Gompertz equation.

  3. Seasonal quantitative dynamics and ecology of pelagic rotifers in an acidified boreal lake

    Directory of Open Access Journals (Sweden)

    Svein Birger Wærvågen

    2017-12-01

    Full Text Available Lake Gjerstadvann is a dimictic, oligotrophic, slightly acidified boreal lake in southern Norway (northwest Europe. The planktonic rotifer community of this lake was studied quantitatively during one year in order to investigate the impacts of the local environment and biotic interactions on seasonal succession and habitat selection. Pure suspension feeders (mainly Keratella spp., Conochilus spp., and Kellicottia longispina together with raptorial graspers or specialised feeders (mainly Polyarthra spp. and Collotheca spp. dominated the rotifer community over prolonged periods, whereas carnivorous/omnivorous species (mainly Asplanchna priodonta were extremely uncommon. Low bicarbonate buffering capacity resulted in a distinctive seasonal oscillating pH between 5.0 and 5.6, defining a special acid-transition lake category. The pH values were highest in the productive period during summer, and lowest during ice break-up coinciding with the peak reactive aluminium concentrations of 250-300 mg L-1. As in typical Norwegian boreal perch lakes, the most abundant cladoceran was Bosmina longispina due to perch predation on the genus Daphnia. Rotifer community structure was significantly related to temperature and oxygen (P=0.001 and P=0.022, illustrating the important effects of the seasonal cycle and vertical density stratification. The most significant competition indicator species were B. longispina and Eudiaptomus gracilis (both with P=0.001. A variance partitioning indicated that 14% of the total community composition variance could only be explained by biotic interactions, while 19% of the variance could be attributed to environmental gradients. Of the variance, 23% could not be resolved between biotic interactions and environmental gradients, while a residual of 44% was not explainable by any of the variables. Acid conditions alone cannot account for all the observed changes in the rotifer community of this lake with low humic content, since

  4. Bioerosion Accretion Replicate (BAR) data covering in situ calcification and bioerosion rates along pH gradients at two volcanically acidified reefs in Papua New Guinea from 2013-01-18 to 2014-11-10 (NCEI Accession 0156692)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Bioerosion Accretion Replicate (BAR) data covering in situ calcification and bioerosion rates along pH gradients at two volcanically acidified reefs in Papua New...

  5. Elevated Colonization of Microborers at a Volcanically Acidified Coral Reef.

    Science.gov (United States)

    Enochs, Ian C; Manzello, Derek P; Tribollet, Aline; Valentino, Lauren; Kolodziej, Graham; Donham, Emily M; Fitchett, Mark D; Carlton, Renee; Price, Nichole N

    2016-01-01

    Experiments have demonstrated that ocean acidification (OA) conditions projected to occur by the end of the century will slow the calcification of numerous coral species and accelerate the biological erosion of reef habitats (bioerosion). Microborers, which bore holes less than 100 μm diameter, are one of the most pervasive agents of bioerosion and are present throughout all calcium carbonate substrates within the reef environment. The response of diverse reef functional groups to OA is known from real-world ecosystems, but to date our understanding of the relationship between ocean pH and carbonate dissolution by microborers is limited to controlled laboratory experiments. Here we examine the settlement of microborers to pure mineral calcium carbonate substrates (calcite) along a natural pH gradient at a volcanically acidified reef at Maug, Commonwealth of the Northern Mariana Islands (CNMI). Colonization of pioneer microborers was higher in the lower pH waters near the vent field. Depth of microborer penetration was highly variable both among and within sites (4.2-195.5 μm) over the short duration of the study (3 mo.) and no clear relationship to increasing CO2 was observed. Calculated rates of biogenic dissolution, however, were highest at the two sites closer to the vent and were not significantly different from each other. These data represent the first evidence of OA-enhancement of microboring flora colonization in newly available substrates and provide further evidence that microborers, especially bioeroding chlorophytes, respond positively to low pH. The accelerated breakdown and dissolution of reef framework structures with OA will likely lead to declines in structural complexity and integrity, as well as possible loss of essential habitat.

  6. Elevated Colonization of Microborers at a Volcanically Acidified Coral Reef.

    Directory of Open Access Journals (Sweden)

    Ian C Enochs

    Full Text Available Experiments have demonstrated that ocean acidification (OA conditions projected to occur by the end of the century will slow the calcification of numerous coral species and accelerate the biological erosion of reef habitats (bioerosion. Microborers, which bore holes less than 100 μm diameter, are one of the most pervasive agents of bioerosion and are present throughout all calcium carbonate substrates within the reef environment. The response of diverse reef functional groups to OA is known from real-world ecosystems, but to date our understanding of the relationship between ocean pH and carbonate dissolution by microborers is limited to controlled laboratory experiments. Here we examine the settlement of microborers to pure mineral calcium carbonate substrates (calcite along a natural pH gradient at a volcanically acidified reef at Maug, Commonwealth of the Northern Mariana Islands (CNMI. Colonization of pioneer microborers was higher in the lower pH waters near the vent field. Depth of microborer penetration was highly variable both among and within sites (4.2-195.5 μm over the short duration of the study (3 mo. and no clear relationship to increasing CO2 was observed. Calculated rates of biogenic dissolution, however, were highest at the two sites closer to the vent and were not significantly different from each other. These data represent the first evidence of OA-enhancement of microboring flora colonization in newly available substrates and provide further evidence that microborers, especially bioeroding chlorophytes, respond positively to low pH. The accelerated breakdown and dissolution of reef framework structures with OA will likely lead to declines in structural complexity and integrity, as well as possible loss of essential habitat.

  7. Use of acidifier and solubilizer in tadalafil solid dispersion to enhance the in vitro dissolution and oral bioavailability in rats.

    Science.gov (United States)

    Choi, Jin-Seok; Kwon, Soon-Hyung; Lee, Sang-Eun; Jang, Woo Suk; Byeon, Jong Chan; Jeong, Hyeong Mo; Park, Jeong-Sook

    2017-06-30

    The purpose of this study is to improve the solubility, in vitro dissolution, and oral bioavailability in rats of tadalafil (TDF) by using SD technique with a weak acid and a copolymer. TDF-SD was prepared via solvent evaporation, coupled with the incorporation of an acidifier and solubilizer. Tartaric acid enhanced the solubility of TDF over 5-fold in DW, and Soluplus ® enhanced the solubility of TDF over 8.7-fold and 19.2-fold compared to that of TDF (pure) in DW and pH 1.2 for 1h, respectively. The optimal formulation of TDF-SD3 was composed of TDF vs Tartaric acid vs Soluplus ® vs Aerosil=1:1:3:3. The in vitro dissolution rate of TDF-SD3 in DW, pH 1.2 and pH 6.8 buffer (51.5%, 53.3%, and 33.2%, respectively) was significantly higher than that of the commercial product (Cialis ® ) powder (16.5%, 15.2%, and 14.8%, respectively). TDF was completely transformed to an amorphous form as shown in SEM, DSC and PXRD data. The stability of TDF-SD3 included drug contents and in vitro dissolution for 1 month were similar to those of Cialis ® , and the amorphous form of TDF-SD3 was well maintained for 6 months. The TDF-SD3 formulation improved the relative bioavailability (BA) and peak plasma concentration (C max ) compared to that of Cialis ® powder after oral administration in rats as 117.3% and 135.7%, respectively. From the results, we found that the acidifier increased the wettability of TDF, and the solubilizer improved solubility through hydrogen bonding with TDF, thereby increasing the solubility, dissolution and oral bioavailability of TDF in TDF-SD3. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Growth potential of Clostridium perfringens from spores in acidified beef, pork, and poultry products during chilling.

    Science.gov (United States)

    Juneja, Vijay K; Baker, David A; Thippareddi, H; Snyder, O Peter; Mohr, Tim B

    2013-01-01

    The ability of Clostridium perfringens to germinate and grow in acidified ground beef as well as in 10 commercially prepared acidified beef, pork, and poultry products was assessed. The pH of ground beef was adjusted with organic vinegar to achieve various pH values between 5.0 and 5.6; the pH of the commercial products ranged from 4.74 to 6.35. Products were inoculated with a three-strain cocktail of C. perfringens spores to achieve ca. 2-log (low) or 4-log (high) inoculum levels, vacuum packaged, and cooled exponentially from 54.4 to 7.2°C for 6, 9, 12, 15, 18, or 21 h to simulate abusive cooling; the U.S. Department of Agriculture, Food Safety and Inspection Service (USDA-FSIS) recommends a cooling time of 6.5 h. Total germinated C. perfringens populations were determined after plating on tryptose-sulfite-cycloserine agar and incubating the plates anaerobically at 37°C for 48 h. In addition, C. perfringens growth from spores was assessed at an isothermal temperature of 44°C. Growth from spores was inhibited in ground beef with a pH of 5.5 or below, even during extended cooling from 54.4 to 7.2°C in 21 h. In ground beef with a pH of 5.6, the growth was >1 log after 18 h of cooling from 54.4 to 7.2°C. However, 15 h of cooling controlled the growth to product with a pH ranging from 4.74 to 5.17, both during exponential abusive cooling periods of up to 21 h and during storage for 21 h at 44°C. While product cooled exponentially from 54.4 to 7.2°C in 15 h or less, the pH 6.35 product supported growth, even after 6 h of cooling from 54.4 to 7.2°C. These challenge tests demonstrate that adjustment of ground beef to pH of 5.5 or less and of barbeque products to pH of 5.63 or less inhibits C. perfringens spore germination and outgrowth during extended cooling periods from 54.4 to 7.2°C up to 15 h. Therefore, safe cooling periods for products with homogeneous, lower pHs can be substantially longer.

  9. Species diversity and metabolic impact of the microbiota are low in spontaneously acidified Belgian sausages with an added starter culture of Staphylococcus carnosus.

    Science.gov (United States)

    Janssens, M; Myter, N; De Vuyst, L; Leroy, F

    2012-04-01

    Quality of fermented sausages is affected by acidifying lactic acid bacteria (LAB) and colour- and flavour-promoting coagulase-negative staphylococci (CNS), whether or not used as starter culture. Artisan fermented sausages are often perceived as superior to industrial variants, partially because of the specific microbiota due to spontaneous acidification, which may be considered as an artisan characteristic. Therefore, two kinds of spontaneously acidified Belgian sausages were prepared (Belgian-type salami and Boulogne sausage), but with addition of a Staphylococcus carnosus culture. The Belgian-type salami was made from pork and beef, whereas the Boulogne sausage contained pork and horse meat. In all cases, Lactobacillus sakei was the dominant LAB species present on the raw materials and during fermentation, whereas enterococci remained present in the background. Enterobacteriaceae vanished after fermentation. The CNS species diversity on the raw materials was large and differed between the pork, beef, and horse meat. Nevertheless, this species diversity was annihilated during fermentation by the added S. carnosus culture. The volatiles fraction was mainly composed of aldehydes that originated from lipid oxidation and spices-derived compounds. Aromatic compounds that are typically associated to CNS activity, such as end-products from the metabolism of branched-chain amino acids, were not present in the Belgian-type salami and only marginally present in the Boulogne sausage. In conclusion, spontaneous acidification of Belgian-type fermented sausages leads to dominance of L. sakei and is no guarantee for bacterial contribution to the aroma profile when S. carnosus is added as a starter culture. Copyright © 2011 Elsevier Ltd. All rights reserved.

  10. Digging deep: Burlington extracts enormous wealth from enormous depths

    Energy Technology Data Exchange (ETDEWEB)

    Smith, M.

    2003-05-01

    Production successes achieved by Burlington Resources Inc., from the deepest operating onshore gas field in the Rocky Mountain region are discussed. The wells in question are located in the Wind River Basin of Wyoming; they are over 25,000-feet deep; they are not only the deepest, but at 290 mmcf of gas per day, the handful of six producing wells are responsible for about 0.5 per cent of total U.S.gas output. Value of the monthly output per well is estimated in the millions of dollars at current natural gas prices; based on estimated reserves of better than 2.8 tcf, the wells are expected to keep flowing for at least two decades. The area is considered to be the harshest operating environment in the industry: searing temperatures of up to 430 degrees F are combined with pressures of 10,400 lbs/sq in, and corrosive concentrations of 12 per cent hydrogen sulfide in an unusual combination with 19 per cent carbon dioxide. To add to the challenges on both the drilling and producing sides, there is also ammonia, unusual in itself, and atypically high pressures in formations above the producing formation. Cost cutting innovations introduced by Burlington in wellbore and casing design and other new approaches, including oil-based drilling fluids, drilling bit improvements, and reverse cementing, all of which were key to the company's success, are also reviewed. On the production side, there is a brief description of the special metallurgy, double-walled pipeline and highly corrosion-resistant alloy tubular products that were developed to deal with the highly corrosive gas stream flowing at wellhead temperatures in excess of 300 degrees F.

  11. Effects of acidification on metal accumulation by aquatic plants and invertebrates. 1. Constructed wetlands

    Science.gov (United States)

    Albers, P.H.; Camardese, M.B.

    1993-01-01

    The pH of lake water is often inversely correlated with concentrations of trace metals in the water column. Concentrations of Al, Cd, Ca, Cu, Fe, Hg, Pb, Mg, Mn, Ni, P, and Zn were compared in water, plants, and aquatic insects from three acidified (pH 5.0) and three nonacidified (pH 6.5) constructed wetlands. Concentrations of Zn in water and bur-reed (Sparganium americanum) were higher in acidified wetlands than in nonacidified wetlands. Floating nonrooted plants contained mean concentrations of Fe, Mg, and Mn that were higher than recommended maximum levels for poultry feed. The mean concentrations of all metals in insects were below recommended maximum levels for poultry feed and below levels that cause toxic effects in wild birds. Smaller than expected increases of metal concentrations in the water of acidified wetlands were probably due to limited mobilization of metals from the sediments and insignificant changes in sedimentation of aqueous metals. Calcium was lower in acidified than in nonacidified wetland water, but the Ca content of insects and bur-reed was not lower. Low concentrations of Ca in aquatic insects from both groups of wetlands indicate that calcium-rich crustaceans and mollusks are probably important to female waterfowl and their young during the spring, when invertebrates make up the majority of the diet. Although toxic effects from metal ingestion seem to be unlikely consequences of wetland acidification, the adverse effect of low pH on the occurrence of crustaceans and mollusks could threaten egg production and development of young.

  12. Sphagnum mosses as a microhabitat for invertebrates in acidified lakes and the colour adaptation and substrate preference in Leucorrhinia dubia (Odonata, Anisoptera)

    Energy Technology Data Exchange (ETDEWEB)

    Henrikson, B.-I. (Dept. of Zoology, Sect. of Animal Ecology, Univ. of Goeteborg, Goeteborg (Sweden))

    1993-01-01

    The increase of peat mosses, Sphagnum spp., in acidified lakes leads to a changed microhabitat structure for benthic invertebrates. The importance of this change was investigated for some benthic invertebrates. Comparisons between quantitative samples of Sphagnum and debris within the acidified Lake Stora Haestevatten, in the Lake Gaardsjoen catchment of SW Sweden, showed significantly higher abundances of Chironomidae, Ceratopogonidae, Odonata, Trichoptera, Cladocera and Argyroneta aquatica (Araneae) in Sphagnum. For chironomidae and Cladocera the differences were tenfold. Special reference was made to the libellulid Leucorrhinia dubia which is common in acid lakes. In a laboratory test, late instar larvae of L. dubia were shown to change colour to correspond to the brown and green colour of Sphagnum. This result was completed with a field test where larvae of L. dubia were significantly more common in Sphagnum of the same colour as the larvae. The ability to change colour may have an adaptive value when coexisting with visual predators. Small larvae were more prevalent in Sphagnum and they also showed a preference for this substrate in the laboratory test. Laboratory tests showed mediumsized larvae preferred Sphagnum. Larvae of L. dubia were more successful as predators on Asellus aquaticus in Sphagnum substrate than in debris in the laboratory test. Laboratory predation tests with notonecta glauca, Corixa dentipes, Acilius sulcatus, Hyphydrus ovatus and L. dubia showed that they could all feed on larvae of L. dubia. The complex habitat structure of Sphagnum is probably the reason for the high abundance of invertebrates since it may serve as both shelter against predation and as foraging sites. it is probably important as a key habitat for young instars of, for example, L. dubia. In lakes with large Sphagnum mats, L. dubia can coexist with fish. The expansion of Sphagnum due to acidification will probably benefit many acid-tolerant invertebrate species. (au)

  13. Disrupting Hypoxia-Induced Bicarbonate Transport Acidifies Tumor Cells and Suppresses Tumor Growth.

    Science.gov (United States)

    McIntyre, Alan; Hulikova, Alzbeta; Ledaki, Ioanna; Snell, Cameron; Singleton, Dean; Steers, Graham; Seden, Peter; Jones, Dylan; Bridges, Esther; Wigfield, Simon; Li, Ji-Liang; Russell, Angela; Swietach, Pawel; Harris, Adrian L

    2016-07-01

    Tumor hypoxia is associated clinically with therapeutic resistance and poor patient outcomes. One feature of tumor hypoxia is activated expression of carbonic anhydrase IX (CA9), a regulator of pH and tumor growth. In this study, we investigated the hypothesis that impeding the reuptake of bicarbonate produced extracellularly by CA9 could exacerbate the intracellular acidity produced by hypoxic conditions, perhaps compromising cell growth and viability as a result. In 8 of 10 cancer cell lines, we found that hypoxia induced the expression of at least one bicarbonate transporter. The most robust and frequent inductions were of the sodium-driven bicarbonate transporters SLC4A4 and SLC4A9, which rely upon both HIF1α and HIF2α activity for their expression. In cancer cell spheroids, SLC4A4 or SLC4A9 disruption by either genetic or pharmaceutical approaches acidified intracellular pH and reduced cell growth. Furthermore, treatment of spheroids with S0859, a small-molecule inhibitor of sodium-driven bicarbonate transporters, increased apoptosis in the cell lines tested. Finally, RNAi-mediated attenuation of SLC4A9 increased apoptosis in MDA-MB-231 breast cancer spheroids and dramatically reduced growth of MDA-MB-231 breast tumors or U87 gliomas in murine xenografts. Our findings suggest that disrupting pH homeostasis by blocking bicarbonate import might broadly relieve the common resistance of hypoxic tumors to anticancer therapy. Cancer Res; 76(13); 3744-55. ©2016 AACR. ©2016 American Association for Cancer Research.

  14. Viscosidade extensional e em cisalhamento de suspensões acidificadas de amido de amaranto e caseinato de sódio Extensional and shear viscosity of acidified amaranth starch-sodium caseinate suspensions

    Directory of Open Access Journals (Sweden)

    Angela Maria Gozzo

    2009-09-01

    Full Text Available Foram avaliadas as viscosidades extensional e em cisalhamento de suspensões acidificadas de amido de amaranto-caseinato de sódio. Sistemas mistos de amido de amaranto-caseinato de sódio acidificados com glucona-delta-lactona (GDL foram estudados por ensaios reológicos em compressão biaxial e cisalhamento. Os efeitos da velocidade de acidificação (lenta e rápida e pH final (neutro e no ponto isoelétrico da caseína foram avaliados considerando as interações entre os biopolímeros e sua consequente influência nos parâmetros reológicos. Todas as amostras apresentaram comportamento pseudoplástico, no entanto, a adição de caseinato de sódio nas suspensões de amido, em pH neutro, promoveu um efeito negativo sobre a viscosidade aparente. Amostras acidificadas apresentaram um aumento na complexidade do sistema devido à formação da rede de amido e caseína, observando que a força necessária para o escoamento foi sempre maior para as amostras contendo concentrações maiores de caseinato. Isso mostra que a agregação e gelificação da proteína promovidas pela acidificação, impediram a microsseparação de fases. Esta rede foi mais forte em sistemas gelificados lentamente, devido à formação de uma rede de proteína mais organizada. Apesar da técnica de compressão biaxial imperfeita ser limitada para avaliação de determinados sistemas, neste estudo, mostrou ser um modo prático e eficiente de se mensurar o comportamento reológico.Extensional and shear viscosity of acidified amaranth starch-sodium caseinate suspensions were evaluated. Mixed systems of amaranth starch-sodium caseinate acidified with glucone-delta-lactone (GDL were studied using rheological measurements under biaxial compression and shear. The effects of the acidification rate (slow and fast and final pH (neutral and isoelectric point of casein were evaluated considering the interactions between biopolymers and their influence on the rheological parameters

  15. Reconstructing pre-acidification pH for an acidified Scottish loch: A comparison of palaeolimnological and modelling approaches

    International Nuclear Information System (INIS)

    Battarbee, R.W.; Monteith, D.T.; Juggins, S.; Evans, C.D.; Jenkins, A.; Simpson, G.L.

    2005-01-01

    We reconstruct the pre-acidification pH of the Round Loch of Glenhead for 1800 AD using three diatom-pH transfer functions and a diatom-cladocera modern analogue technique (MAT), and compare these palaeo-data with hindcast data for the loch using the dynamic catchment acidification model MAGIC. We assess the accuracy of the transfer functions by comparing pH inferences from contemporary sediment and sediment trap diatom samples from the lake with measured pH from the UK Acid Waters Monitoring Network. The results from the transfer functions estimate the pH in 1800 to have been between 5.5. and 5.7, the MAT approach estimates pH at 5.8 and the MAGIC hindcast (for 1850) is pH 6.1. Whilst we have no independent method of assessing which of these values is most accurate, the disagreement between the two approaches indicates that further work is needed to resolve the discrepancies. - Methods of reconstructing pre-acidification pH for an acidified Scottish loch compared

  16. Prophylactic effect of rebamipide on aspirin-induced gastric lesions and disruption of tight junctional protein zonula occludens-1 distribution.

    Science.gov (United States)

    Suzuki, Takahiro; Yoshida, Norimasa; Nakabe, Nami; Isozaki, Yutaka; Kajikawa, Hirokazu; Takagi, Tomohisa; Handa, Osamu; Kokura, Satoshi; Ichikawa, Hiroshi; Naito, Yuji; Matsui, Hirofumi; Yoshikawa, Toshikazu

    2008-03-01

    Aspirin and nonsteroidal anti-inflammatory agents are known to induce gastroduodenal complications such as ulcer, bleeding, and dyspepsia. In this study, we examined the prophylactic effect of rebamipide, an anti-ulcer agent with free-radical scavenging and anti-inflammatory effect, on acidified aspirin-induced gastric mucosal injury in rats. In addition, we investigated the mucosal barrier functions disrupted by aspirin. Oral administration of acidified aspirin resulted in linear hemorrhagic erosions with increasing myeloperoxidase activity and thiobarbituric acid-reactive substance concentrations in the gastric mucosa. Rebamipide suppressed these acidified aspirin-induced gastric lesions and inflammatory changes significantly, and its protective effect was more potent in the case of repeated (twice daily for 3 days) treatment than single treatment before aspirin administration. Immunostaining of zonula occludens (ZO)-1, one of the tight junctional proteins, was strengthened in rat gastric mucosa after repeated administration of rebamipide. In addition, aspirin induced the increasing transport of fluorescine isothiocyanate-labeled dextrans with localized disruption and decreased expression of ZO-1 protein on rat gastric mucosal cell line RGM-1. Rebamipide effectively prevented aspirin-induced permeability changes and disruption of ZO-1 distribution. These results suggest that rebamipide protects against aspirin-induced gastric mucosal lesions by preserving gastric epithelial cell-to cell integrity in addition to the anti-inflammatory effects.

  17. Pulse shaping in the presence of enormous second-order dispersion in Al:ZnO/ZnO epsilon-near-zero metamaterial

    Science.gov (United States)

    Kelly, Priscilla; Kuznetsova, Lyuba

    2018-04-01

    A numerical study of the ultra-short pulse propagation in the aluminum-doped zinc oxide multi-layered metamaterial at the epsilon-near-zero spectral point is presented. The Drude model for dielectric permittivity and comparison with recent experimental data predict that damping frequency γD has the highest impact on the material losses and results in enormous second-order dispersion. Numerical simulations using both, the finite-difference time domain algorithm and the split-step Fourier method, show that variations of group velocity across the pulse at the epsilon-near-zero point results in a unique "soliton-like" propagation regime without nonlinearity for the propagation lengths of up to 300 nm.

  18. Effect of corn particle size and inclusion of organic acid in the diet on growth performance and gastrointestinal structure in young chicks

    Directory of Open Access Journals (Sweden)

    Baldassare Fronte

    2013-12-01

    Full Text Available The effect of 3 corn particle sizes (dgw: 375, 1117, and 2402 µm combined with or without organic acids (0.3 g/kg of Galliacid S® was investigated on broilers from Day 1 to Day 21; 540 1-day old Ross 708 males were raised in 36 pens (3x2 factorial design, 6 blocks each. We measured: body weight, feed intake, feed conversion ratio, liver weight, pH weight and height of empty gizzard, pH and length of intestine and caeca, height and width of ileal villi, crypt depth/gland diameter, total bacteria count. Different corn particle sizes and organic acid supplements only affected feed intake (Days 14 and 21, feed conversion ratio (Day 14, villus height, and crypt depth. On Day 21, fine milling had negative effects on body weights compared with larger feed particle size (816 vs 848 and 844 g; acidic additive had a positive effect on broiler growth (859 vs 813 g. Length of small intestinal villi and crypt depth were affected by both particle size and organic acids (fine to coarse small intestinal villi: 1869a, 1401c, and 1039d µm in non-acidified; 1708b, 1535c, and 942e µm in acidified. Fine to coarse crypt depth: 102ab, 98b; 65c µm in non-acidified; 106a, 70c, and 66c µm in acidified. No difference was observed in total bacteria counts of the gut in relation to the different treatments. Use of organic acids during starter phase is useful, especially when the milling process is inappropriate.

  19. Protective effects of astaxanthin from Paracoccus carotinifaciens on murine gastric ulcer models.

    Science.gov (United States)

    Murata, Kenta; Oyagi, Atsushi; Takahira, Dai; Tsuruma, Kazuhiro; Shimazawa, Masamitsu; Ishibashi, Takashi; Hara, Hideaki

    2012-08-01

    The purpose of this study was to investigate the effect of astaxanthin extracted from Paracoccus carotinifaciens on gastric mucosal damage in murine gastric ulcer models. Mice were pretreated with astaxanthin for 1 h before ulcer induction. Gastric ulcers were induced in mice by oral administration of hydrochloride (HCl)/ethanol or acidified aspirin. The effect of astaxanthin on lipid peroxidation in murine stomach homogenates was also evaluated by measuring the level of thiobarbituric acid reactive substance (TBARS). The free radical scavenging activities of astaxanthin were also measured by electron spin resonance (ESR) measurements. Astaxanthin significantly decreased the extent of HCl/ethanol- and acidified aspirin-induced gastric ulcers. Astaxanthin also decreased the level of TBARS. The ESR measurement showed that astaxanthin had radical scavenging activities against the 1,1-diphenyl-2-picrylhydrazyl radical and the superoxide anion radical. These results suggest that astaxanthin has antioxidant properties and exerts a protective effect against ulcer formation in murine models. Copyright © 2011 John Wiley & Sons, Ltd.

  20. Thermal inactivation of Salmonella Enteritidis on chicken skin previously exposed to acidified Sodium chlorite or tri-sodium phosphate.

    Science.gov (United States)

    Karuppasamy, K; Yadav, Ajit S; Saxena, Gaurav K

    2015-12-01

    Thermal inactivation of normal and starved cells of Salmonella Enteritidis on chicken skin previously exposed to different concentrations of acidified sodium chlorite (ASC) or tri-sodium phosphate (TSP) was investigated. Inoculated skin was pretreated with different concentration of ASC or TSP, packaged in bags, and then immersed in a circulating water bath at 60 to 68 °C. The recovery medium was Hektoen enteric agar. D-values, determined by linear regression, for normal cells on chicken skin, were 2.79, 1.17 and 0.53 min whereas D-values for starved cells were 4.15, 1.83 and 0.66 at 60, 64 and 68 °C, respectively. z-values for normal cells were 3.54 and for starved cells were 2.29. Pretreatment of Salmonella Enteritidis cells with 0 to 200 ppm of ASC or 0 to 1.0 % TSP resulted in lower D-values at all temperatures. Sensory results indicated no significance differences for control and treatments. Thus, results of this study indicated that pretreatment of chicken skin with ASC or TSP increased sensitivity of Salmonella Enteritidis to heat without affecting organoleptic quality of chicken meat.

  1. Modifications of Carbonate Fracture Hydrodynamic Properties by CO 2 -Acidified Brine Flow

    Energy Technology Data Exchange (ETDEWEB)

    Deng, Hang; Ellis, Brian R.; Peters, Catherine A.; Fitts, Jeffrey P.; Crandall, Dustin; Bromhal, Grant S.

    2013-08-15

    Acidic reactive flow in fractures is relevant in subsurface activities such as CO{sub 2} geological storage and hydraulic fracturing. Understanding reaction-induced changes in fracture hydrodynamic properties is essential for predicting subsurface flows such as leakage, injectability, and fluid production. In this study, x-ray computed tomography scans of a fractured carbonate caprock were used to create three dimensional reconstructions of the fracture before and after reaction with CO{sub 2}-acidified brine (Ellis et al., 2011, Greenhouse Gases: Sci. Technol., 1:248-260). As expected, mechanical apertures were found to increase substantially, doubling and even tripling in some places. However, the surface geometry evolved in complex ways including ‘comb-tooth’ structures created from preferential dissolution of calcite in transverse sedimentary bands, and the creation of degraded zones, i.e. porous calcite-depleted areas on reacted fracture surfaces. These geometric alterations resulted in increased fracture roughness, as measured by surface Z{sub 2} parameters and fractal dimensions D{sub f}. Computational fluid dynamics (CFD) simulations were conducted to quantify the changes in hydraulic aperture, fracture transmissivity and permeability. The results show that the effective hydraulic apertures are smaller than the mechanical apertures, and the changes in hydraulic apertures are nonlinear. Overestimation of flow rate by a factor of two or more would be introduced if fracture hydrodynamic properties were based on mechanical apertures, or if hydraulic aperture is assumed to change proportionally with mechanical aperture. The differences can be attributed, in part, to the increase in roughness after reaction, and is likely affected by contiguous transverse sedimentary features. Hydraulic apertures estimated by the 1D statistical model and 2D local cubic law (LCL) model are consistently larger than those calculated from the CFD simulations. In addition, a novel

  2. Emission estimates for some acidifying and greenhouse gases and options for their control in Finland

    Energy Technology Data Exchange (ETDEWEB)

    Pipatti, R. [VTT Energy, Espoo (Finland). Energy Systems

    1998-11-01

    This thesis presents estimates and options for control of anthropogenic ammonia (NH{sub 3}), methane (CH{sub 4}), nitrous oxide (N{sub 2}O) and some halocarbon emissions in Finland. Ammonia is an air pollutant which contributes to both acidification and nitrogen eutrophication of ecosystems. Its emissions are mainly caused by livestock manure. In Finland the anthropogenic emissions of NH{sub 3} have been estimated to be approximately 44 Gg in 1985 and 43 Gg in 1990. In the 1990`s the emissions have declined due to the reduced number of cattle and voluntary implementation of emission reducing measures. The impact of NH{sub 3} emissions on acidification is serious but in Finland it is less than the impact of the other acidifying gases sulphur dioxide (SO{sub 2}) and nitrogen oxides (NO{sub x}). All three gases and their transformation products are transported by the atmosphere up to distances of hundreds or even more than a thousand kilometres. NH{sub 3} emissions can be reduced with relatively cost-effective measures and the measures can partly replace the implementation of more costly abatement measures on SO{sub 2} and NO{sub x} emissions needed to lower the acidifying deposition in Finland. The other gases studied in this thesis are greenhouse gases. Some of the gases also deplete stratospheric ozone. Finnish anthropogenic CH{sub 4} emissions have been estimated to be around 250 Gg per year during the 1990`s. The emissions come mainly from landfills and agricultural sources (enteric fermentation and manure). The significance of other CH{sub 4} sources in Finland is minor. The potential to reduce the Finnish CH{sub 4} emissions is estimated to be good. Landfill gas recovery offers an option to reduce the emissions significantly at negligible cost if the energy produced can be utilised in electricity and/or heat production. Measures directed at reducing the emissions from livestock manure management are more costly, and the achievable reduction in the emissions

  3. Effect of bismuth citrate, lactose, and organic acid on necrotic enteritis in broilers

    Science.gov (United States)

    Clostridium perfringens – associated necrotic enteritis causes significant losses and increased morbidity in poultry. The objective of this study was to evaluate the effect of bismuth citrate and acidifiers on the development of necrotic enteritis in broilers. The first study was a dose response t...

  4. Acid or N? Disentangling Nutrient- and pH Effects of Nitrogen and Sulfur Deposition to Forest Ecosystems

    Science.gov (United States)

    Goodale, C. L.

    2016-12-01

    Nitrogen deposition can act as both a nutrient and acidifying agent with sometimes synergistic and sometimes contradictory effects on ecosystem processes. However, these two roles are rarely separated. Similarly, deposition patterns of N and S often covary, making it difficult to correctly attribute their respective roles on the biogeochemistry of downwind ecosystems. In 2011, we initiated a N x pH (S) experiment in six mixed hardwood stands (3 primary, 3 secondary) in Central New York designed to separate nutrient and acidifying impacts of N and S deposition. Three treatments included two 50 N ha-1 yr-1 additions in forms intended to raise (NaNO3) or lower ((NH4)2SO4) soil pH as well as elemental S treatment to acidify without N. Five years of treatment shifted surface soil pH in the expected directions. Treatment effects on soil extract DOC correlated with pH, with lower DOC concentration and aromaticity in the acidifying treatments. Foliar litterfall did not vary by stand age or treatment, but N and S treatments enriched litterfall N and S concentrations, respectively. Wood production did not vary significantly by stand age or treatment but trended toward an increase in response to the N additions in both stand ages. The treatments did not affect early stages of litter decomposition, but both N additions and acidification suppressed decomposition in later stages, with largest effects from acidification alone. Soil respiration responses followed those of litter decomposition, except that the response of respiration to the NaNO3 addition depended on the stand's mycorrhizal composition, with greater suppression in stands with a higher fraction of ectomycorrhizal tree species. Together, these results show that both N addition and acidification can suppress decomposition rates, but likely for different reasons that may be linked to plant carbon allocation (for N) and microbial function (pH). Distinguishing these mechanisms will be important for projecting recovery of

  5. Retrospective evaluation of methionine intoxication associated with urinary acidifying products in dogs: 1,525 cases (2001-2012).

    Science.gov (United States)

    Hickey, Mara C; Son, Tolina T; Wismer, Tina

    2015-01-01

    To describe the signalment, clinical findings, timing of signs, outcome, and prognosis in a population of dogs exposed to methionine through the ingestion of urine acidifying products. Retrospective observational study from January 1, 2001 to December 31, 2012. Animal Poison Control Center. A total of 1,197 case calls yielding 1,525 dogs identified with presumed methionine ingestion. None. Records of dogs with presumptive methionine ingestion were reviewed from the American Society for the Prevention of Cruelty to Animals Animal Poison Control Center database. Ingested methionine doses ranged from 3.9 mg/kg to 23,462 mg/kg. Clinical signs developed in 47% of dogs. The most common clinical signs were gastrointestinal (GI) and neurologic. The mean onset of GI signs was 2.8 hours following ingestion. The mean onset of neurologic signs was 6.8 hours following ingestion. GI signs were identified with ingested doses ≥22.5 mg/kg. Vomiting was the most common GI sign. Neurologic signs were identified with ingested doses ≥94.6 mg/kg. Ataxia was the most common neurologic sign. Resolution of clinical signs occurred within 48 hours of ingestion, and no fatalities were reported. Prognosis for dogs with methionine intoxication is excellent. Vomiting and ataxia were the most common clinical signs associated with methionine toxicosis. © Veterinary Emergency and Critical Care Society 2015.

  6. Predicting long-term recovery of a strongly acidified stream using MAGIC and climate models (Litavka, Czech Republic

    Directory of Open Access Journals (Sweden)

    D. W. Hardekopf

    2008-03-01

    Full Text Available Two branches forming the headwaters of a stream in the Czech Republic were studied. Both streams have similar catchment characteristics and historical deposition; however one is rain-fed and strongly affected by acid atmospheric deposition, the other spring-fed and only moderately acidified. The MAGIC model was used to reconstruct past stream water and soil chemistry of the rain-fed branch, and predict future recovery up to 2050 under current proposed emissions levels. A future increase in air temperature calculated by a regional climate model was then used to derive climate-related scenarios to test possible factors affecting chemical recovery up to 2100. Macroinvertebrates were sampled from both branches, and differences in stream chemistry were reflected in the community structures. According to modelled forecasts, recovery of the rain-fed branch will be gradual and limited, and continued high levels of sulphate release from the soils will continue to dominate stream water chemistry, while scenarios related to a predicted increase in temperature will have little impact. The likelihood of colonization of species from the spring-fed branch was evaluated considering the predicted extent of chemical recovery. The results suggest that the possibility of colonization of species from the spring-fed branch to the rain-fed will be limited to only the acid-tolerant stonefly, caddisfly and dipteran taxa in the modelled period.

  7. Effect of CO2-related acidification on aspects of the larval development of the European lobster, Homarus gammarus (L.

    Directory of Open Access Journals (Sweden)

    D. Boothroyd

    2009-08-01

    Full Text Available Oceanic uptake of anthropogenic CO2 results in a reduction in pH termed "Ocean Acidification" (OA. Comparatively little attention has been given to the effect of OA on the early life history stages of marine animals. Consequently, we investigated the effect of culture in CO2-acidified sea water (approx. 1200 ppm, i.e. average values predicted using IPCC 2007 A1F1 emissions scenarios for year 2100 on early larval stages of an economically important crustacean, the European lobster Homarus gammarus. Culture in CO2-acidified sea water did not significantly affect carapace length of H. gammarus. However, there was a reduction in carapace mass during the final stage of larval development in CO2-acidified sea water. This co-occurred with a reduction in exoskeletal mineral (calcium and magnesium content of the carapace. As the control and high CO2 treatments were not undersaturated with respect to any of the calcium carbonate polymorphs measured, the physiological alterations we record are most likely the result of acidosis or hypercapnia interfering with normal homeostatic function, and not a direct impact on the carbonate supply-side of calcification per se. Thus despite there being no observed effect on survival, carapace length, or zoeal progression, OA related (indirect disruption of calcification and carapace mass might still adversely affect the competitive fitness and recruitment success of larval lobsters with serious consequences for population dynamics and marine ecosystem function.

  8. Can the eastern red-backed salamander (Plethodon cinereus) persist in an acidified landscape?

    Science.gov (United States)

    Bondi, Cheryl A; Beier, Colin M.; Ducey, Peter K; Lawrence, Gregory B.; Bailey, Scott W.

    2016-01-01

    Hardwood forests of eastern North America have experienced decades of acidic deposition, leading to soil acidification where base cation supply was insufficient to neutralize acid inputs. Negative impacts of soil acidity on amphibians include disrupted embryonic development, lower growth rates, and habitat loss. However, some amphibians exhibit intraspecific variation in acid tolerance, suggesting the potential for local adaptation in areas where soils are naturally acidic. The eastern red-backed salamander (Plethodon cinereus) is a highly abundant top predator of the northern hardwood forest floor. Early research found that P. cinereus was sensitive to acidic soils, avoiding substrates with pH habitats. However, recent studies have documented P. cinereus populations in lower pH conditions than previously observed, suggesting some populations may persist in acidic conditions. Here, we evaluated relationships between organic horizon soil pH and P. cinereus abundance, adult health (body size and condition), and microhabitat selection, based on surveys of 34 hardwood forests in northeastern United States that encompass a regional soil pH gradient. We found no associations between soil pH and P. cinereus abundance or health, and observed that this salamander used substrates with pH similar to that available, suggesting that pH does not mediate their fine-scale distributions. The strongest negative predictor of P. cinereus abundance was the presence of dusky salamanders (Desmognathus spp.), which were most abundant in the western Adirondacks. Our results indicate that P. cinereus occupies a wider range of soil pH than has been previously thought, which has implications for their functional role in forest food webs and nutrient cycles in acid-impaired ecosystems. Tolerance of P. cinereus for more acidic habitats, including anthropogenically acidified forests, may be due to local adaptation in reproductively isolated populations and/or generalist

  9. A systematic review of the effectiveness of liming to mitigate impacts of river acidification on fish and macro-invertebrates

    International Nuclear Information System (INIS)

    Mant, Rebecca C.; Jones, David L.; Reynolds, Brian; Ormerod, Steve J.; Pullin, Andrew S.

    2013-01-01

    The addition of calcium carbonate to catchments or watercourses – liming – has been used widely to mitigate freshwater acidification but the abatement of acidifying emissions has led to questions about its effectiveness and necessity. We conducted a systematic review and meta-analysis of the impact of liming streams and rivers on two key groups of river organisms: fish and invertebrates. On average, liming increased the abundance and richness of acid-sensitive invertebrates and increased overall fish abundance, but benefits were variable and not guaranteed in all rivers. Where B-A-C-I designs (before-after-control-impact) were used to reduce bias, there was evidence that liming decreased overall invertebrate abundance. This systematic review indicates that liming has the potential to mitigate the symptoms of acidification in some instances, but effects are mixed. Future studies should use robust designs to isolate recovery due to liming from decreasing acid deposition, and assess factors affecting liming outcomes. -- Highlights: •In a systematic review and meta-analysis, we asked how river liming affected fish and invertebrates. •On average, liming increased fish abundance. •Liming also increased average abundance and richness of acid-sensitive invertebrates. •However, benefits were variable and not guaranteed in all acidified rivers. -- A systematic review showed lime application to acidified rivers increased average fish abundance, and abundance and richness in acid-sensitive invertebrates, but not always

  10. Effects of Neutralization and Crosslinking Agents on the Morphology of Chitosan Electrospun Scaffolds

    Directory of Open Access Journals (Sweden)

    Maryam Mashayekhi

    2017-01-01

    Full Text Available Chitosan, a natural polymer derived from chitin by deacetylation process of chitin, has gained an enormous interest in tissue engineering due to its unique features such as antibacterial activity and wound healing properties. Electrospinning of acidified chitosan solution is one of the most widely-used approaches in fabrication of 3D scaffolds. Although there are some reports addressing morphology tailoring of the chitosan nanofibers through solution electrospinning, there is no comparative report concerning the neutralization and stabilization conditions of chitosan electrospun fibers. Therefore, this article compares the effects of different neutralizing agents such as aqueous solutions of sodium carbonate (Na2CO3 and potassium carbonate (K2CO3, and crosslinking reagents including glutaraldehyde (GA and genipin on morphology of electrospun chitosan fibers. After neutralization and stabilization processes, Fourier transform infrared spectroscopy (FTIR was employed to investigate the morphology of fibers. Furthermore, the influence of the aforementioned parameters on stability of fibers was probed using scanning electron microscopy. SEM images illustrated that the scaffold resulting from electrospinning of 4 wt% chitosan solution in a mixture of trifluoroacetic acid (TFA and dichloromethane (DCM possessed a well-formed nanofibrous structure. Afterwards, different methods for neutralization and stabilization of the electrospun chitosan nanofiber mats were performed. In this respect, aqueous solutions of both Na2CO3 and K2CO3 salts (1M were employed as neutralization agents and GA and genipin were used as two different crosslinking agents. Based on SEM analysis, the chitosan fibers, crosslinked with genipin, showed better morphology than a scaffold which was crosslinked with glutaraldehyde

  11. Dermal application of nitric oxide releasing acidified nitrite-containing liniments significantly reduces blood pressure in humans.

    Science.gov (United States)

    Opländer, Christian; Volkmar, Christine M; Paunel-Görgülü, Adnana; Fritsch, Thomas; van Faassen, Ernst E; Mürtz, Manfred; Grieb, Gerrit; Bozkurt, Ahmet; Hemmrich, Karsten; Windolf, Joachim; Suschek, Christoph V

    2012-02-15

    Vascular ischemic diseases, hypertension, and other systemic hemodynamic and vascular disorders may be the result of impaired bioavailability of nitric oxide (NO). NO but also its active derivates like nitrite or nitroso compounds are important effector and signal molecules with vasodilating properties. Our previous findings point to a therapeutical potential of cutaneous administration of NO in the treatment of systemic hemodynamic disorders. Unfortunately, no reliable data are available on the mechanisms, kinetics and biological responses of dermal application of nitric oxide in humans in vivo. The aim of the study was to close this gap and to explore the therapeutical potential of dermal nitric oxide application. We characterized with human skin in vitro and in vivo the capacity of NO, applied in a NO-releasing acidified form of nitrite-containing liniments, to penetrate the epidermis and to influence local as well as systemic hemodynamic parameters. We found that dermal application of NO led to a very rapid and significant transepidermal translocation of NO into the underlying tissue. Depending on the size of treated skin area, this translocation manifests itself through a significant systemic increase of the NO derivates nitrite and nitroso compounds, respectively. In parallel, this translocation was accompanied by an increased systemic vasodilatation and blood flow as well as reduced blood pressure. We here give evidence that in humans dermal application of NO has a therapeutic potential for systemic hemodynamic disorders that might arise from local or systemic insufficient availability of NO or its bio-active NO derivates, respectively. Copyright © 2012 Elsevier Inc. All rights reserved.

  12. Restoring life to acidified lakes

    Energy Technology Data Exchange (ETDEWEB)

    Shepard, M

    1986-05-01

    In 1983 EPRI initiated the lake acidification mitigation project (LAMP) in order to examine the long-term ecosystem effects of liming lakes, and to develop a model for calculating optimal liming doses. Investigations were carried out at lakes under 3 sets of conditions: reacidification, maintenance liming and preventive maintenance liming. The research so far has indicated that liming is a safe and effective technique.

  13. Granulated wood ash to forest soil - Ecological effects

    International Nuclear Information System (INIS)

    Rosen, K.; Eriksson, H.; Clarholm, M.; Lundkvist, H.; Rudebeck, A.

    1993-01-01

    This report describes research concerning ecological effects of wood ash recycling to forest soils. The main part of the minerals in the wood fuels are retained in the ashes after combustion. By returning the ashes back to the cleared forest areas, the mineral losses can be reduced. Adding ashes and limestone is a method to vitalize acidified forest soils and restore the production capacity. 48 refs, 26 figs, 8 tabs

  14. Efeito de três fertilizantes acidificantes sobre a concentração de alumínio e de manganês em folhas e raízes de cafeeiros Effect of three acidifying fertilizers on the concentration of aluminum and manganese on coffee leaves and roots

    Directory of Open Access Journals (Sweden)

    Ferdinando Roberto Pupo de Moraes

    1979-01-01

    Full Text Available Foi determinada a concentração de alumínio e de manganês em folhas de cafeeiros cultivados em vasos com três solos diferentes e com aplicação de três fertilizantes nitrogenados acidificantes (nitrato de amônio, uréia e sulfato de amônio e um não acidificante (salitre-do-chile, além de dois tratamentos extras com corretivos de acidez e um com enxofre. Nas raízes estes elementos foram também determinados para os tratamentos que receberam uréia, uréia mais enxofre e o controle sem nitrogênio. Nos três solos determinou-se o efeito dos tratamentos sobre o pH e a concentração de A1(3+ e Mn3+. A análise foliar revelou diferenças significativas entre tratamentos na concentração de alumínio e manganês das folhas e em todos os três solos estudados. Alguns sintomas específicos observados nas folhas e raízes estiveram associados à presença de níveis elevados de manganês nessas partes vegetais.There were determined concentrations of aluminum and manganese in leaves of coffee trees cultivated in pots with 3 different soils fertilized with acidifying nitrogen fertilizers (ammonium nitrate, urea and ammonium sulphate and non acidifying nitrogen fertilizer (chilean nitrate and 3 other treatments (urea plus calcium carbonate, urea plus lime and urea plus sulfur. Aluminum and manganese were determined in coffee roots of pots fertilized with urea, urea plus sulfur and a control without nitrogen. The relation of pH and concentration of Al3+ and Mn2+ are discussed for the soils utilized. Leaves analysis showed significant differences in aluminum and manganese concentration in the leaves among treatments and among the 3 soils types. Symptoms correlated with high levels of manganese in the leaves and roots of the coffee trees were observed.

  15. Enormous enhancement of ZnO nanorod photoluminescence

    International Nuclear Information System (INIS)

    Wang, Y.H.; Duan, W.J.; Wu, Z.L.; Zheng, D.; Zhou, X.W.; Zhou, B.Y.; Dai, L.J.; Wang, Y.S.

    2012-01-01

    ZnO nanorod arrays were grown on quartz slices in the aqueous solution of zinc acetate and hexamethylenetetramine at 90 °C. Then ZnO:Mg shells were epitaxially grown on the nanorods to form core/shell structures in the aqueous solution of zinc acetate, magnesium acetate and hexamethylenetetramine at the same temperature. Effects of the shells and UV laser beam irradiation on the crystal structure and photoluminescence properties of ZnO nanorods were studied. ZnO:Mg shells suppress the green emission and enhance the UV emission intensity of the nanorods by 38 times. Enhancement of the UV emission depends on the Mg content in the shells. Short time UV laser beam irradiation could improve ZnO nanorod emission efficiently. The UV emission intensity of ZnO nanorods is enhanced by 71 times by capping and subsequent UV laser beam irradiation. - Highlights: ► ZnO nanorod arrays were grown on quartz slices in solution at 90 °C. ► The nanorods were capped by ZnO:Mg layers to form core/shell structures. ► ZnO:MgO shells suppress the green emission and enhance the UV emission intensity by 38 times. ► The enhancement depends on the Mg content in the shells. ► Exposing the nanorods to 325 laser beam improves the UV emission efficiently. ► Capping and 325 nm laser beam irradiation could enhance the nanorod UV emission intensity by 71 times.

  16. The effect of different detanninfication effects on the tannins found in ...

    African Journals Online (AJOL)

    200604877

    2013-12-18

    Dec 18, 2013 ... Int. J. Crude Drug Res. 23, 87-90. Barry, T.N. ... Analysis of condensed tannins using Acidified Vanillin. J. Sci. Food. Agr. 29, 788-794. .... Interactions among tannins, supplementation and polyethylene glycol in goats given oak ...

  17. Stable Carbon Isotope Characterization of CO2 Loss in Acid Mine Drainage Impacted Stream Water: Observations from a Laboratory Experiment

    Science.gov (United States)

    Ali, H. N.; Atekwana, E. A.

    2007-05-01

    Water from an acid mine drainage spring, ground water from a mine tailings pile, stream water and tap water were acidified to simulate acid mine drainage (AMD) contamination. The objective was to determine how acidification of stream water by AMD affected DIC loss and carbon isotope fraction. Two 20 L HDP containers (reactors) containing samples from each source were left un-acidified and allowed to evolve under ambient conditions for several weeks in the laboratory and two others were acidified. Acidification was carried out progressively with sulfuric acid to pH <3. For acidified samples, one reactor was acidified open to the atmosphere and the other closed from contact with atmosphere and CO2(g) was collected under vacuum. The un-acidified samples did not show significant alkalinity and DIC loss, and the 13C of DIC was enriched with time. The acidified samples showed decrease in alkalinity and DIC and increase in the 13C of DIC and CO2(g) with progressive acidification. The enrichment of 13C of DIC for un-acidified samples was due to exchange with atmospheric CO2. On the other hand, the 13C enrichment in the acidified samples was due to fractionation during dehydration of HCO3- and diffusive loss of CO2(g) from the aqueous phase. The actual values measured depended on the amount of CO2 lost from the aqueous phase during acidification. Samples with greater CO2 loss (closed acidification) had greater 13C enrichment. Beyond the HCO3- titration end point, the δ13C of DIC and CO2(g) was similar and nearly constant. The result of this study suggests that AMD effects on DIC can be modeled as a first order kinetic reaction and the isotope enrichment modeled using Rayleigh distillation.

  18. The enormous Chillos Valley Lahar: An ash-flow-generated debris flow from Cotopaxi Volcano, Ecuador

    Science.gov (United States)

    Mothes, P.A.; Hall, M.L.; Janda, R.J.

    1998-01-01

    The Chillos Valley Lahar (CVL), the largest Holocene debris flow in area and volume as yet recognized in the northern Andes, formed on Cotopaxi volcano's north and northeast slopes and descended river systems that took it 326 km north-northwest to the Pacific Ocean and 130+ km east into the Amazon basin. In the Chillos Valley, 40 km downstream from the volcano, depths of 80-160 m and valley cross sections up to 337000m2 are observed, implying peak flow discharges of 2.6-6.0 million m3/s. The overall volume of the CVL is estimated to be ???3.8 km3. The CVL was generated approximately 4500 years BP by a rhyolitic ash flow that followed a small sector collapse on the north and northeast sides of Cotopaxi, which melted part of the volcano's icecap and transformed rapidly into the debris flow. The ash flow and resulting CVL have identical components, except for foreign fragments picked up along the flow path. Juvenile materials, including vitric ash, crystals, and pumice, comprise 80-90% of the lahar's deposit, whereas rhyolitic, dacitic, and andesitic lithics make up the remainder. The sand-size fraction and the 2- to 10-mm fraction together dominate the deposit, constituting ???63 and ???15 wt.% of the matrix, respectively, whereas the silt-size fraction averages less than ???10 wt.% and the clay-size fraction less than 0.5 wt.%. Along the 326-km runout, these particle-size fractions vary little, as does the sorting coefficient (average = 2.6). There is no tendency toward grading or improved sorting. Limited bulking is recognized. The CVL was an enormous non-cohesive debris flow, notable for its ash-flow origin and immense volume and peak discharge which gave it characteristics and a behavior akin to large cohesive mudflows. Significantly, then, ash-flow-generated debris flows can also achieve large volumes and cover great areas; thus, they can conceivably affect large populated regions far from their source. Especially dangerous, therefore, are snowclad volcanoes

  19. Effect of pasteurization temperature on stability of an acidified sugarcane juice beverage

    Directory of Open Access Journals (Sweden)

    Mariana Kunitake

    2014-12-01

    Full Text Available The shelf life of fresh sugarcane juice is quite limited due to the high rates of microbiological and enzymatic reactions which take place after extraction. In order to evaluate the impact of pasteurization temperature on quality and stability of sugarcane juice with passion fruit pulp, nine batches of sugarcane juice with 4g/100g passion fruit pulp were processed at 85, 90 and 95 °C for 30 s, in triplicate. The pasteurized beverage was aseptically packaged in polyethylene terephthalate (PET bottles and stored at 7 °C in the dark. The beverage was characterized by physicochemical tests. Activities of polyphenol oxidase (PPO and peroxidase (POD were determined before and after processing. Coliforms and Salmonella tests were carried out to assure the beverage´s microbiological safety. Color parameters were measured in the processed juice throughout the storage period. Fifty panelists evaluated the beverage's appearance, aroma, flavor, and overall impression using seven-point hedonic scale tests. Sensory stability was estimated by considering score averages above four and percentages of acceptance above 60%. The pH, soluble solids and titratable acidity of end product ranged from 3.96 to 4.19, 19.7 to 20.1 ºBrix, and 0.163 to 0.175 g/100g citric acid, respectively. The three processing binomials were effective for PPO inactivation; however, complete POD inactivation was reached at 95 °C/30 s only. The estimated sensory shelf-lives for sugarcane juice with passion fruit pulp processed at 85, 90 and 95 °C/30 s were 30, 40 and 50 days, respectively. Thus, the increase of pasteurization temperature had a positive effect on product's stability.

  20. Effects of Comprehensive Technologies on the Improvement of Acidified Vineyard Soils

    Science.gov (United States)

    Jiang, Hongguo; Wang, Qiunan; Xu, Feng; Jin, Jun; Wang, Guoyu; Liu, Jianguo

    2018-01-01

    Soil acidification is an important factor that restricts the yield and quality of fruits. In this study, the comprehensive improving technologies were applied on the vineyards in which the soil pH is below 5.5. The technologies include application of soil conditioner, organic fertilizer and bacterial manure, and growth of green manure and natural grass. The results show that the comprehensive improving technologies can raise the pH of 0-15 cm soil layer by 0.5-0.8 unit and the pH of 15-30 cm soil layer by 0.3-0.6 unit. The soil bulk densities are decreased by 0.77-10.42%. The contents of organic matter, total N, available P and K in the soils are all increased. Therefore, the soil fertilities are improved. The yields of grape fruits are increased by 12.77-14.94%, and the contents of soluble solid in the grapes are raised by 7.01-9.55%, by the comprehensive measure of seaweed liquid silicon plus sheep manure plus growth of green manure. The comprehensive measure of soil conditioner Naduoli No. 1 plus bacterial manure plus natural grass increases the yields of grape by 7.67%, raises the content of soluble solid in the grape by 8.6%. But the effect of the comprehensive measure of unslaked lime plus sheep manure plus growth of green manure is not clear.

  1. 'It's quite hard to grasp the enormity of it': perceived needs of people upon diagnosis of rheumatoid arthritis.

    Science.gov (United States)

    Radford, S; Carr, M; Hehir, M; Davis, B; Robertson, L; Cockshott, Z; Tipler, S; Hewlett, S

    2008-09-01

    The diagnosis of rheumatoid arthritis (RA) brings rapid pharmacological and multidisciplinary team interventions to address inflammatory processes and symptom management. However, people may also need support on the journey to self-management. The aim of this study was to explore what professional support patients feel they receive upon diagnosis, and what support they feel would be most helpful. Two focus groups comprised patients with at least five years'; disease duration (n = 7), and patients more recently diagnosed (5-18 months, n = 5). The latter had attended at least two appointments in a rheumatology nurse specialist clinic during the previous year, aimed at providing support upon diagnosis. Transcripts were subjected to thematic analysis to identify common issues regarding support needs, which were then grouped into themes. Interviewing and analysis was performed by researchers not involved in clinical care. Four overarching themes emerged. 'Information' was needed about the symptoms of RA, its management and personal outcome, while 'Support' related to emotional needs ('It's quite hard to grasp the enormity of it'). Information and Support overlapped, in that patients wanted someone to talk to, and to be listened to. These two themes were underpinned by issues of service delivery: 'Choice' (patient or professional to talk to, groups, one-to-one) and 'Involvement' (holistic care, partnership), which overlapped in terms of the opportunity to decide when and which interventions to access. People with RA report not only informational, but also emotional support needs at diagnosis. The potential for delivering emotional support to patients around the time of diagnosis warrants further exploration. (c) 2008 John Wiley & Sons, Ltd.

  2. Influence of acidification and aluminium on the density and biomass of lotic benthic invertebrates

    Energy Technology Data Exchange (ETDEWEB)

    Allard, M.; Moreau, G.

    1986-10-01

    Experiments were carried out in plasticized wooden channels fed by a small creek in the Reserve des Laurentides, 80 km north of Quebec city. Channels were naturally colonized by invertebrates for 65 d before treatment. Treated channels were acidified in August with dilute H/sub 2/SO/sub 4/ only, or with acid plus a solution of Al sulfate (final concentration of 0.19 mg L/sup -1/). The control channel received creek water only (pH 6.3 to 6.9). The addition of Al had no effect on invertebrate density and biomass. After 73 d of acidification, invertebrate densities were only one third the number found in the control channel. Invertebrate biomass was not different within channels, although biomass was generally higher in the two acidified channels. Difference in densities between acidified and non-acidified channels was attributed to lack of colonization and not to an increase in drift. Microtendipes, a large and resistant larva of Chironomidae constituted a large fraction of the biomass, largely outweighing numerous very small larvae. Effects on the density were attributed to the direct effect of low pH and not to indirect action through food limitations. 22 refs.

  3. Non-additive effects of ocean acidification in combination with warming on the larval proteome of the Pacific oyster, Crassostrea gigas.

    Science.gov (United States)

    Harney, Ewan; Artigaud, Sébastien; Le Souchu, Pierrick; Miner, Philippe; Corporeau, Charlotte; Essid, Hafida; Pichereau, Vianney; Nunes, Flavia L D

    2016-03-01

    Increasing atmospheric carbon dioxide results in ocean acidification and warming, significantly impacting marine invertebrate larvae development. We investigated how ocean acidification in combination with warming affected D-veliger larvae of the Pacific oyster Crassostrea gigas. Larvae were reared for 40h under either control (pH8.1, 20 °C), acidified (pH7.9, 20 °C), warm (pH8.1, 22 °C) or warm acidified (pH7.9, 22 °C) conditions. Larvae in acidified conditions were significantly smaller than in the control, but warm acidified conditions mitigated negative effects on size, and increased calcification. A proteomic approach employing two-dimensional electrophoresis (2-DE) was used to quantify proteins and relate their abundance to phenotypic traits. In total 12 differentially abundant spots were identified by nano-liquid chromatography-tandem mass spectrometry. These proteins had roles in metabolism, intra- and extra-cellular matrix formations, stress response, and as molecular chaperones. Seven spots responded to reduced pH, four to increased temperature, and six to acidification and warming. Reduced abundance of proteins such as ATP synthase and GAPDH, and increased abundance of superoxide dismutase, occurred when both pH and temperature changes were imposed, suggesting altered metabolism and enhanced oxidative stress. These results identify key proteins that may be involved in the acclimation of C. gigas larvae to ocean acidification and warming. Increasing atmospheric CO2 raises sea surface temperatures and results in ocean acidification, two climatic variables known to impact marine organisms. Larvae of calcifying species may be particularly at risk to such changing environmental conditions. The Pacific oyster Crassostrea gigas is ecologically and commercially important, and understanding its ability to acclimate to climate change will help to predict how aquaculture of this species is likely to be impacted. Modest, yet realistic changes in pH and

  4. Improved quinoa growth, physiological response, and seed nutritional quality in three soils having different stresses by the application of acidified biochar and compost.

    Science.gov (United States)

    Ramzani, Pia Muhammad Adnan; Shan, Lin; Anjum, Shazia; Khan, Waqas-Ud-Din; Ronggui, Hu; Iqbal, Muhammad; Virk, Zaheer Abbas; Kausar, Salma

    2017-07-01

    Quinoa (Chenopodium quinoa Willd.) is a traditional Andean agronomical resilient seed crop having immense significance in terms of high nutritional qualities and its tolerance against various abiotic stresses. However, finite work has been executed to evaluate the growth, physiological, chemical, biochemical, antioxidant properties, and mineral nutrients bioavailability of quinoa under abiotic stresses. Depending on the consistency in the stability of pH, intended rate of S was selected from four rates (0.1, 0.2, 0.3, 0.4 and 0.5% S) for the acidification of biochar and compost in the presence of Thiobacillus thiooxidans by pH value of 4. All three soils were amended with 1% (w/w) acidified biochar (BC A ) and compost (CO A ). Results revealed that selective plant growth, yield, physiological, chemical and biochemical improved significantly by the application of BC A in all stressed soils. Antioxidants in quinoa fresh leaves increased in the order of control > CO A  > BC A , while reactive oxygen species decreased in the order of control < CO A  < BC A . A significant reduction in anti-nutrients (phytate and polyphenols) was observed in all stressed soils with the application of BC A . Moreover, incorporation of CO A and BC A reduced the pH of rhizosphere soil by 0.4-1.6 units in all stressed soils, while only BC A in bulk soil decreased pH significantly by 0.3 units. These results demonstrate that BC A was more effective than CO A to enhance the bioavailability, translocation of essential nutrients from the soil to plant and their enhanced bioavailability in the seed. Copyright © 2017. Published by Elsevier Masson SAS.

  5. Early postnatal diagnosis of hereditary spherocytosis by combining light microscopy, acidified glycerol lysis test and eosin-5'-maleimide binding assay.

    Science.gov (United States)

    Andres, Oliver; Eber, Stefan; Speer, Christian P

    2015-12-01

    Exact diagnosis of hereditary spherocytosis (HS) is widely considered unreliable around birth. However, early postnatal diagnosis at the beginning of congenital hemolysis may be essential for managing neonatal anemia and hemolytic icterus, identifying those at high risk for severe hyperbilirubinemia, irreversible kernicterus, or sudden need for red cell transfusion. We analyzed 37 blood samples from neonates or infants up to six weeks of life that had been collected in-house or shipped to our laboratory due to suspected red cell membrane disorder. By combining assessment of red cell morphology, acidified glycerol lysis test (AGLT), and eosin-5'-maleimide (EMA) binding assay, we were able to clearly exclude HS in 22 and confirm HS in 10 patients, of which one had undergone red cell transfusion prior to blood sampling. Assessment of red cell morphology and normal test results allowed diagnosis of infantile pyknocytosis or Heinz body anemia in three neonates. Re-evaluation of five patients with inconsistent results of AGLT and EMA binding led to confirmation of HS in two cases. Automated analysis of hematologic parameters revealed elevated proportion of hyperdense cells to be a highly significant indicator for HS in neonatal infants. We showed that assessment of red cell morphology in combination with AGLT and EMA binding assay is a reliable basis for confirming or rejecting suspected diagnosis of HS even in neonates. Our data underline the necessity for blood sampling and laboratory exploration in suspected red cell membrane or enzyme defects at the earliest occasion.

  6. Cultivation Versus Molecular Analysis of Banana (Musa sp.) Shoot-Tip Tissue Reveals Enormous Diversity of Normally Uncultivable Endophytic Bacteria.

    Science.gov (United States)

    Thomas, Pious; Sekhar, Aparna Chandra

    2017-05-01

    The interior of plants constitutes a unique environment for microorganisms with various organisms inhabiting as endophytes. Unlike subterranean plant parts, aboveground parts are relatively less explored for endophytic microbial diversity. We employed a combination of cultivation and molecular approaches to study the endophytic bacterial diversity in banana shoot-tips. Cultivable bacteria from 20 sucker shoot-tips of cv. Grand Naine included 37 strains under 16 genera and three phyla (Proteobacteria, Actinobacteria, Firmicutes). 16S rRNA gene-ribotyping approach on 799f and 1492r PCR-amplicons to avoid plant organelle sequences was ineffective showing limited bacterial diversity. 16S rRNA metagene profiling targeting the V3-V4 hypervariable region after filtering out the chloroplast (74.2 %), mitochondrial (22.9 %), and unknown sequences (1.1 %) revealed enormous bacterial diversity. Proteobacteria formed the predominant phylum (64 %) succeeded by Firmicutes (12.1 %), Actinobacteria (9.5 %), Bacteroidetes (6.4 %), Planctomycetes, Cyanobacteria, and minor shares (banana shoot-tips (20 phyla, 46 classes) with about 2.6 % of the deciphered 269 genera and 1.5 % of the 656 observed species from the same source of shoot-tips attained through cultivation. The predominant genera included several agriculturally important bacteria. The study reveals an immense ecosystem of endophytic bacteria in banana shoot tissues endorsing the earlier documentation of intracellular "Cytobacts" and "Peribacts" with possible roles in plant holobiome and hologenome.

  7. Characterisation of lignins isolated from sugarcane bagasse pretreated with acidified ethylene glycol and ionic liquids

    International Nuclear Information System (INIS)

    Moghaddam, Lalehvash; Zhang, Zhanying; Wellard, R. Mark; Bartley, John P.; O'Hara, Ian M.; Doherty, William O.S.

    2014-01-01

    Sugarcane bagasse pretreatment processes using acidified aqueous ethylene glycol (EG) and ionic liquids (ILs) have been reported recently. In this study, recovery of lignins from these processes was conducted, as well as determination of their physico-chemical properties. The amount of lignins recovered from 1-butyl-3-methylimidazolium chloride ([bmim]Cl) with HCl as a catalyst and [bmim][CH 3 SO 3 ] was ∼42%, and ∼35%–36% by EG with HCl or H 2 SO 4 as a catalyst, respectively. The isolated lignins were characterised using wet chemistry, spectroscopy and thermogravimetry analysis (TGA), and the results compared to soda lignin from NaOH pretreatment of bagasse. The IL and EG lignins contained no or trace amounts of carbohydrates, slightly lower hydrogen content but slightly higher oxygen contents than soda lignin. The IL and EG lignins contained more C-3 and C-5 reactive sites for Mannich reaction and had more p-hydroxypheny propane unit structures than soda lignin. Two-dimensional heteronuclear single quantum coherence (2D HSQC) nuclear magnetic resonance (NMR) identified the major substructural units in the lignins, and allowed differences among them to be studied. As EG lignins were extracted in very reactive environment, intermediate enol ethers were formed and led to cleavage reactions which were not apparent in the other lignins. 31 P NMR and infra-red spectroscopy results showed that IL and EG lignins had lower total hydroxyl content than soda lignin, probably indicating that a higher degree of self-polymerisation occurred during bagasse pretreatment, despite the use of lower temperature and shorter reaction time. On the basis of the salient features of these lignins, potential applications were proposed. - Highlights: • Lignins were recovered from ethylene glycol (EG) and ionic liquid (IL) processes. • IL and EG lignins contained no or trace amounts of carbohydrates. • IL and EG lignin had more C-3 and C-5 sites for Mannich reaction than soda

  8. Acidified seawater increases accumulation of cobalt but not cesium in manila clam Ruditapes philippinarum.

    Science.gov (United States)

    Sezer, Narin; Kocaoğlan, Hasan Oğuz; Kılıç, Önder; Lacoue-Labarthe, Thomas; Belivermiş, Murat

    2018-04-01

    The pH of seawater around the world is expected to continue its decline in the near future in response to ocean acidification that is driven by heightened atmospheric CO 2 emissions. Concomitantly, economically-important molluscs that live in coastal waters including estuaries and embayments, may be exposed to a wide assortment of contaminants, including trace metals and radionuclides. Seawater acidification may alter both the chemical speciation of select elements as well as the physiology of organisms, and may thus pose at risk to many shellfish species, including the manila clam Ruditapes philippinarum. The bioconcentration efficiency of two common radionuclides associated with the nuclear fuel cycle, 134 Cs and 57 Co, were investigated by exposing live clams to dissolved 134 Cs and 57 Co at control (pH = 8.1) and two lowered pH (pH = 7.8 and 7.5) levels using controlled aquaria. The uptake and depuration kinetics of the two radionuclides in the whole-body clam were followed for 21 and 35 days, respectively. At steady-state equilibrium, the concentration factor (CF ss ) for 57 Co increased as the pH decreased (i.e. 130 ± 5, 194 ± 6, and 258 ± 10 at pH levels 8.1, 7.8 and 7.5, respectively), whereas the 134 Cs uptake was not influenced by a change in pH conditions. During depuration, the lowest depuration rate constant of 57 Co by the manila clam was observed at the intermediate pH of 7.8. An increase in the accumulation of 57 Co at the intermediate pH value was thought to be caused mainly by the aragonitic shell of the clam, as well as the low salinity and alkalinity of seawater used in the experiment. Considering that accumulation consists of uptake and depuration, among the three pH conditions moderately acidified seawater enhanced most the accumulation of 57 Co. Accumulation of 134 Cs was not strongly influenced by a reduced pH condition, as represented by an analogous uptake constant rate and CF ss in each treatment. Such results suggest that

  9. A Putative ABC Transporter Permease Is Necessary for Resistance to Acidified Nitrite and EDTA in Pseudomonas aeruginosa under Aerobic and Anaerobic Planktonic and Biofilm Conditions.

    Science.gov (United States)

    McDaniel, Cameron; Su, Shengchang; Panmanee, Warunya; Lau, Gee W; Browne, Tristan; Cox, Kevin; Paul, Andrew T; Ko, Seung-Hyun B; Mortensen, Joel E; Lam, Joseph S; Muruve, Daniel A; Hassett, Daniel J

    2016-01-01

    Pseudomonas aeruginosa (PA) is an important airway pathogen of cystic fibrosis and chronic obstructive disease patients. Multiply drug resistant PA is becoming increasing prevalent and new strategies are needed to combat such insidious organisms. We have previously shown that a mucoid, mucA22 mutant PA is exquisitely sensitive to acidified nitrite ([Formula: see text], pH 6.5) at concentrations that are well tolerated in humans. Here, we used a transposon mutagenesis approach to identify PA mutants that are hypersensitive to [Formula: see text]. Among greater than 10,000 mutants screened, we focused on PA4455, in which the transposon was found to disrupt the production of a putative cytoplasmic membrane-spanning ABC transporter permease. The PA4455 mutant was not only highly sensitive to [Formula: see text], but also the membrane perturbing agent, EDTA and the antibiotics doxycycline, tigecycline, colistin, and chloramphenicol, respectively. Treatment of bacteria with [Formula: see text] plus EDTA, however, had the most dramatic and synergistic effect, with virtually all bacteria killed by 10 mM [Formula: see text], and EDTA (1 mM, aerobic, anaerobic). Most importantly, the PA4455 mutant was also sensitive to [Formula: see text] in biofilms. [Formula: see text] sensitivity and an anaerobic growth defect was also noted in two mutants (rmlC and wbpM) that are defective in B-band LPS synthesis, potentially indicating a membrane defect in the PA4455 mutant. Finally, this study describes a gene, PA4455, that when mutated, allows for dramatic sensitivity to the potential therapeutic agent, [Formula: see text] as well as EDTA. Furthermore, the synergy between the two compounds could offer future benefits against antibiotic resistant PA strains.

  10. Effects of experimental acidification on mobilisation of metals from sediments of limed and non-limed lakes

    International Nuclear Information System (INIS)

    Waellstedt, Teresia; Borg, Hans

    2003-01-01

    In order to study the influence of pH on the mobilisation of metals from lake sediments, intact sediment cores with overlying water were sampled from one lime treated lake and one acidified lake. The overlying water of two cores from each lake was successively acidified to pH 4.2 over a period of 3 months. In the acid treated samples from the limed lake, the initial concentrations of Al, Cd, Mn, Pb and Zn in the overlying water were generally lower and the final concentrations were higher than in the acid treated samples from the acidified lake. The labile inorganic fraction of Al (Al i ) was increasingly dominating as pH decreased. Redox potential and pH in the sediment indicated that the upper two centimetres were involved in the exchange reactions. The experiment showed that mobilisation of metals from sediments can occur and the results indicated that mobilisation could contribute to increased concentrations of metals in lake water during reacidification of formerly lime treated lakes. - Reacidification leads to mobilisation of metals from lake sediments

  11. Climate change and our responsibilities as chemists

    Directory of Open Access Journals (Sweden)

    Bassam Z. Shakhashiri

    2014-01-01

    Full Text Available For almost all of 4.5 billion years, natural forces have shaped Earth’s environment. But, during the past century, as a result of the Industrial Revolution, which has had enormous benefits for humans, the effects of human activities have become the main driver for climate change. The increase of atmospheric carbon dioxide caused by burning fossil fuels for energy to power the revolution causes an energy imbalance between incoming solar radiation and outgoing planetary emission. The imbalance is warming the planet and causing the atmosphere and oceans to warm, ice to melt, sea level to rise, and weather extremes to increase. In addition, dissolution of part of the carbon dioxide in the oceans is causing them to acidify, with possible negative effects on marine biota. As citizens of an interconnected global society and scientists who have the background to understand climate change, we have a responsibility first to understand the science. One resource that is available to help is the American Chemical Society Climate Science Toolkit, www.acs.org/climatescience. With this understanding our further responsibility as citizen scientists is to engage others in deliberative discussions on the science, to take actions ourselves to adapt to and mitigate human-caused climate change, and urge others to follow our example.

  12. Hypoxia and acidification have additive and synergistic negative effects on the growth, survival, and metamorphosis of early life stage bivalves.

    Science.gov (United States)

    Gobler, Christopher J; DePasquale, Elizabeth L; Griffith, Andrew W; Baumann, Hannes

    2014-01-01

    Low oxygen zones in coastal and open ocean ecosystems have expanded in recent decades, a trend that will accelerate with climatic warming. There is growing recognition that low oxygen regions of the ocean are also acidified, a condition that will intensify with rising levels of atmospheric CO2. Presently, however, the concurrent effects of low oxygen and acidification on marine organisms are largely unknown, as most prior studies of marine hypoxia have not considered pH levels. We experimentally assessed the consequences of hypoxic and acidified water for early life stage bivalves (bay scallops, Argopecten irradians, and hard clams, Mercenaria mercenaria), marine organisms of significant economic and ecological value and sensitive to climate change. In larval scallops, experimental and naturally-occurring acidification (pH, total scale  = 7.4-7.6) reduced survivorship (by >50%), low oxygen (30-50 µM) inhibited growth and metamorphosis (by >50%), and the two stressors combined produced additively negative outcomes. In early life stage clams, however, hypoxic waters led to 30% higher mortality, while acidified waters significantly reduced growth (by 60%). Later stage clams were resistant to hypoxia or acidification separately but experienced significantly (40%) reduced growth rates when exposed to both conditions simultaneously. Collectively, these findings demonstrate that the consequences of low oxygen and acidification for early life stage bivalves, and likely other marine organisms, are more severe than would be predicted by either individual stressor and thus must be considered together when assessing how ocean animals respond to these conditions both today and under future climate change scenarios.

  13. Effect of acid Lugol solution as preservative on two representative chitineous and gelatinous zooplankton groups

    DEFF Research Database (Denmark)

    Jaspers, Cornelia; Carstensen, Jacob

    2009-01-01

    The estimation of biomass from body lengths to carbon regressions is a common approach in plankton research. Several different chemicals for sample preservation are in use, and conversion factors to account for shrinkage effects exist, but to our knowledge the consequences of using potassium......-iodide and iodine (Lugol solution) as preservative on body sizes of different mesozooplankton groups have not been investigated. We tested the effect of 2% acidified Lugol solution on body sizes over time on two major marine mesozooplankton groups, namely larvaceans and copepods, which are representatives...

  14. Effects of liming on forage availability and nutrient content in a forest impacted by acid rain.

    Directory of Open Access Journals (Sweden)

    Sarah E Pabian

    Full Text Available Acidic deposition and subsequent forest soil acidification and nutrient depletion can affect negatively the growth, health and nutrient content of vegetation, potentially limiting the availability and nutrient content of forage for white-tailed deer (Odocoileus virginianus and other forest herbivores. Liming is a mitigation technique that can be used to restore forest health in acidified areas, but little is known about how it affects the growth or nutrient content of deer forage. We examined the effects of dolomitic limestone application on the growth and chemical composition of understory plants in an acidified forest in central Pennsylvania, with a focus on vegetative groups included as white-tailed deer forage. We used a Before-After-Control-Impact study design with observations 1 year before liming and up to 5 years post-liming on 2 treated and 2 untreated 100-ha sites. Before liming, forage availability and several nutrients were below levels considered optimal for white-tailed deer, and many vegetative characteristics were related to soil chemistry. We observed a positive effect of liming on forb biomass, with a 2.7 fold increase on limed sites, but no biomass response in other vegetation groups. We observed positive effects of liming on calcium and magnesium content and negative effects on aluminum and manganese content of several plant groups. Responses to liming by forbs and plant nutrients show promise for improving vegetation health and forage quality and quantity for deer.

  15. An Examination of the Correlative Effects of IT Outsourcing with IT Agility, IT Strategic Alignment and IT Effectiveness

    Science.gov (United States)

    Eichman, Bruce W.

    2013-01-01

    Organizational executives are concerned with the insufficient alignment of Information Technology (IT) investments to achieve computed based information systems effectiveness. Survey results of senior executives determined that in spite of applying enormous amounts of resources and energy attempting to prioritize and effectively align these…

  16. Piper umbellatum L.: A medicinal plant with gastric-ulcer protective and ulcer healing effects in experimental rodent models.

    Science.gov (United States)

    da Silva Junior, Iberê Ferreira; Balogun, Sikiru Olaitan; de Oliveira, Ruberlei Godinho; Damazo, Amílcar Sabino; Martins, Domingos Tabajara de Oliveira

    2016-11-04

    Piper umbellatum L. (Piperaceae) is a shrub found in the Amazon, Savannah and Atlantic Forest region of Brazil. It is widely used in folk medicine in many countries primarily for the treatment of gastric disorders. The aim of this study was to evaluate the gastroprotective and anti-ulcer effects of hydroethanolic extract of P. umbellatum (HEPu) leaves in experimental rodents. In addition, the anti-Helicobacter pylori activity of the extract was assessed. The leaves of P. umbellatum were macerated in 75% (1:3w/v) hydroethanolic solution to obtain HEPu. The gastroprotective and ulcer healing activities of HEPu were evaluated using acidified ethanol (acute) and acetic acid (chronic) gastric ulcer models in rodents. The anti-H. pylori activity was evaluated by in vitro broth microdilution assay using H. pylori cagA + and vacA + strain. The probable mechanism of action of HEPu was evaluated by determining gastric secretory parameters, antioxidant enzyme (catalase), non-protein sulfhydryl (glutathione) and malondialdehyde levels in gastric tissue, including pro-inflammatory (IL-1β, TNF-a, IL -17, RANTES, IFN-γ and MIP-2) and anti-inflammatory (IL-10) cytokines. HEPu demonstrated potent gastroprotection against acute ulcer induced by acidified ethanol and excellent healing effect of the chronic ulcer induced by acetic acid. The gastroprotective activity in acidified ethanol is partly attributed to the antioxidant mechanisms, while anti-secretory, anti-inflammatory and regeneration of the gastric mucosa are evoked as part of its antiulcer mechanism of action. The gastric ulcer healing of HEPu also involves restoration of the altered cytokines levels to near normal. However, it has no in vitro anti-H. pylori activity. The results of this study showed that HEPu possesses preventive and curative effects in experimental models of gastric ulcers in animals. These effects are partially dependent on antioxidant, antisecretory, anti-inflammatory and mucosa regeneration. It is

  17. Foliar Nutrient Distribution Patterns in Sympatric Maple Species Reflect Contrasting Sensitivity to Excess Manganese.

    Science.gov (United States)

    Fernando, Denise R; Marshall, Alan T; Lynch, Jonathan P

    2016-01-01

    Sugar maple and red maple are closely-related co-occurring tree species significant to the North American forest biome. Plant abiotic stress effects including nutritional imbalance and manganese (Mn) toxicity are well documented within this system, and are implicated in enhanced susceptibility to biotic stresses such as insect attack. Both tree species are known to overaccumulate foliar manganese (Mn) when growing on unbuffered acidified soils, however, sugar maple is Mn-sensitive, while red maple is not. Currently there is no knowledge about the cellular sequestration of Mn and other nutrients in these two species. Here, electron-probe x-ray microanalysis was employed to examine cellular and sub-cellular deposition of excessively accumulated foliar Mn and other mineral nutrients in vivo. For both species, excess foliar Mn was deposited in symplastic cellular compartments. There were striking between-species differences in Mn, magnesium (Mg), sulphur (S) and calcium (Ca) distribution patterns. Unusually, Mn was highly co-localised with Mg in mesophyll cells of red maple only. The known sensitivity of sugar maple to excess Mn is likely linked to Mg deficiency in the leaf mesophyll. There was strong evidence that Mn toxicity in sugar maple is primarily a symplastic process. For each species, leaf-surface damage due to biotic stress including insect herbivory was compared between sites with acidified and non-acidified soils. Although it was greatest overall in red maple, there was no difference in biotic stress damage to red maple leaves between acidified and non-acidified soils. Sugar maple trees on buffered non-acidified soil were less damaged by biotic stress compared to those on unbuffered acidified soil, where they are also affected by Mn toxicity abiotic stress. This study concluded that foliar nutrient distribution in symplastic compartments is a determinant of Mn sensitivity, and that Mn stress hinders plant resistance to biotic stress.

  18. Salmonella enterica suppresses Pectobacterium carotovorum subsp. carotovorum population and soft rot progression by acidifying the microaerophilic environment.

    Science.gov (United States)

    Kwan, Grace; Charkowski, Amy O; Barak, Jeri D

    2013-02-12

    and human hosts. Their populations are higher on plants cocolonized with the common bacterial soft rot pathogen Pectobacterium carotovorum subsp. carotovorum, turning edible plants into a risk factor for human disease. We inoculated leaves with P. carotovorum subsp. carotovorum and S. enterica or E. coli O157:H7 to study the interactions between these bacteria. While P. carotovorum subsp. carotovorum enhanced the growth of both S. enterica and E. coli O157:H7, these human pathogens affected P. carotovorum subsp. carotovorum fundamentally differently. S. enterica reduced P. carotovorum subsp. carotovorum growth and acidified the environment, leading to less soft rot on leaves; E. coli O157:H7 had no such effects. As soft rot signals a food safety risk, the reduction of soft rot symptoms in the presence of S. enterica may lead consumers to eat healthy-looking but S. enterica-contaminated produce.

  19. Effect of acidified drinking water on the recovery of Salmonella enteritidis from broiler crops

    Directory of Open Access Journals (Sweden)

    Avila LAF de

    2003-01-01

    Full Text Available Crop is a known source of Salmonella contamination during broiler carcass processing. The effect of drinking water acidification by lactic acid or citric acid or a combination of those with cupric sulfate and d-limonene in the reduction of Salmonella Enteritidis (SE recovered from the crop of broilers was evaluated. Treatments were administered during 8 hours of preslaughter fasting period (Experiments I and II and during the last 32 hours of preslaughter (Experiment III. It was observed that acidification reduced water intake when treatments began at preslaughter feed withdrawal, and affected the possible reducing effect of these acids on SE recovering (Experiments I and II. Water intake during preslaughter feed withdrawal was not affected when treatment began 32 hours before slaughter (Experiment III. Treatments reduced SE recovering from crop (p<0.05. In Experiment III, 0.470% of lactic acid reduced the number of recovered SE in 99%. This study suggested that the addition of organic acids in the drinking water 24 hours before beginning the preslaughter feed withdrawal might reduce crop SE colonization and might be an important strategy to reduce SE contamination of broiler products during processing.

  20. Theoretical experimental study of the factors that govern the molybdenum absorption signal by means of electro thermic atomic absorption spectroscopy

    International Nuclear Information System (INIS)

    Garaboto Farfan, M. A.

    1996-01-01

    The formation of molybdenum carbides in the atomizer, used in the electro thermic atomic absorption spectroscopy, is responsible for incomplete analyte removal in its analysis. This generates the apparition of the memory effect and little precision in the results. In this work, different variables that could affect the molybdenum absorption sign were investigated, as well as the influence of hydrochloric acid on the memory effect, by means of studies in the different stages: drying, calcination and atomization, and the samples deposition order in molybdenum solutions, either acidified or not acidified [es

  1. Stability of free and immobilized Lactobacillus acidophilus and Bifidobacterium lactis in acidified milk and of immobilized B. lactis in yoghurt Estabilidade de Lactobacillus acidophilus e Bifidobacterium lactis nas formas livre e imobilizada em leite acidificado e de B. lactis imobilizado em iogurte

    Directory of Open Access Journals (Sweden)

    Carlos Raimundo Ferreira Grosso

    2004-06-01

    Full Text Available This study evaluated the stability of Bifidobacterium lactis (Bb-12 and of Lactobacillus acidophilus (La-05 both free and immobilized in calcium alginate, in milk and in acidified milk (pH 5.0, 4.4 and 3.8. The stability of immobilized B. lactis in yoghurt (fermented to pH 4.2, during 28 days of refrigerated storage was also evaluated. The efficiency of two culture media (modified MRS agar and Reinforced Clostridial Agar plus Prussian Blue for counting of B. lactis in yoghurt was determined. Lee's agar was used to count Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus when B. lactis were counted in the MRS medium. B. lactis and L. acidophilus in both free and immobilized forms presented satisfactory rates of survival in milk and acidified milk because the average reduction of the population was only one log cycle after 21 days of storage. The number of viable cells of immobilized B. lactis in yoghurt presented a gradual decline throughout the storage period, passing from 10(8 cfu/ml to no count after 28 days of storage. When the cultures were not in equilibrium just the selective medium was efficient in counting B. lactis in yoghurt. The results showed that both microorganisms can be added to milk and acidified milk, because their population was only slightly affected during storage. The presence of traditional culture of yoghurt seems to be harmful for survival of immobilized B. lactis and the immobilization in calcium alginate failed as an effective barrier to protect the cells in all analysed treatments.Este trabalho avaliou a estabilidade de Bifidobacterium lactis (Bb-12 e de Lactobacillus acidophilus (La-05 nas formas livre e imobilizada em alginato de cálcio, em leite e leite acidificado (pHs 5.0, 4.4 e 3.8, e a estabilidade de B. lactis imobilizado em iogurte (fermentado até pH 4.2, durante 28 dias de estocagem refrigerada. Também foi estudada a eficiência de dois meios de cultura (ágar MRS modificado e

  2. Effect of strong acids on red mud structural and fluoride adsorption properties.

    Science.gov (United States)

    Liang, Wentao; Couperthwaite, Sara J; Kaur, Gurkiran; Yan, Cheng; Johnstone, Dean W; Millar, Graeme J

    2014-06-01

    The removal of fluoride using red mud has been improved by acidifying red mud with hydrochloric, nitric and sulphuric acid. The acidification of red mud causes sodalite and cancrinite phases to dissociate, confirmed by the release of sodium and aluminium into solution as well as the disappearance of sodalite bands and peaks in infrared and X-ray diffraction data. The dissolution of these mineral phases increases the amount of available iron and aluminium oxide/hydroxide sites that are accessible for the adsorption of fluoride. However, concentrated acids have a negative effect on adsorption due to the dissolution of these iron and aluminium oxide/hydroxide sites. The removal of fluoride is dependent on the charge of iron and aluminium oxide/hydroxides on the surface of red mud. Acidifying red mud with hydrochloric, nitric and sulphuric acid resulted in surface sites of the form ≡SOH2(+) and ≡SOH. Optimum removal is obtained when the majority of surface sites are in the form ≡SOH2(+) as the substitution of a fluoride ion does not cause a significant increase in pH. This investigation shows the importance of having a low and consistent pH for the removal of fluoride from aqueous solutions using red mud. Copyright © 2014 Elsevier Inc. All rights reserved.

  3. Determination of effects of air pollution on natural vegetation. Made-to-measure; Het bepalen van effecten op luchtverontreiniging op natuurlijke vegetaties. Maatwerk

    Energy Technology Data Exchange (ETDEWEB)

    Jeurink, N. [Tauw, Deventer (Netherlands)

    2011-02-15

    Effect prediction of nitrogen deposition is following several steps. After deposition calculations, an ecologist will interpret the possible consequences for natural vegetation. This article describes the manner in which the interpretation of effects is conducted and indicates how the cumulative effect of acidified deposition should be handled. [Dutch] Effectvoorspelling van stikstofdepositie verloopt in enkele stappen. Na depositieberekeningen volgt interpretatie door een ecoloog van de mogelijke gevolgen voor natuurlijke vegetaties. Dit artikel beschrijft de wijze waarop de interpretatie van effecten plaatsvindt en geeft aan hoe daarbij met de cumulatieve invloed van zure depositie moet worden omgegaan.

  4. National implementation of the UNECE convention on long-range transboundary air pollution (effects). Pt. 1. Deposition loads: methods, modelling and mapping results, trends

    Energy Technology Data Exchange (ETDEWEB)

    Gauger, Thomas [Federal Agricultural Research Centre, Braunschweig (DE). Inst. of Agroecology (FAL-AOE); Stuttgart Univ. (Germany). Inst. of Navigation; Haenel, Hans-Dieter; Roesemann, Claus [Federal Agricultural Research Centre, Braunschweig (DE). Inst. of Agroecology (FAL-AOE)

    2008-09-15

    The report on the implementation of the UNECE convention on long-range transboundary air pollution Pt.1, deposition loads (methods, modeling and mapping results, trends) includes the following chapters: Introduction, deposition on air pollutants used for the input for critical loads in exceeding calculations, methods applied for mapping total deposition loads, mapping wet deposition, wet deposition mapping results, mapping dry deposition, dry deposition mapping results, cloud and fog mapping results, total deposition mapping results, modeling the air concentration of acidifying components and heavy metals, agricultural emissions of acidifying and eutrophying species.

  5. Application of lime (CaCO3) to promote forest recovery from severe acidification increases potential for earthworm invasion

    Science.gov (United States)

    Homan, Caitlin; Beirer, Colin M; McCay, Timothy S; Lawrence, Gregory B.

    2016-01-01

    The application of lime (calcium carbonate) may be a cost-effective strategy to promote forest ecosystem recovery from acid impairment, under contemporary low levels of acidic deposition. However, liming acidified soils may create more suitable habitat for invasive earthworms that cause significant damage to forest floor communities and may disrupt ecosystem processes. We investigated the potential effects of liming in acidified soils where earthworms are rare in conjunction with a whole-ecosystem liming experiment in the chronically acidified forests of the western Adirondacks (USA). Using a microcosm experiment that replicated the whole-ecosystem treatment, we evaluated effects of soil liming on Lumbricus terrestris survivorship and biomass growth. We found that a moderate lime application (raising pH from 3.1 to 3.7) dramatically increased survival and biomass of L. terrestris, likely via increases in soil pH and associated reductions in inorganic aluminum, a known toxin. Very few L. terrestris individuals survived in unlimed soils, whereas earthworms in limed soils survived, grew, and rapidly consumed leaf litter. We supplemented this experiment with field surveys of extant earthworm communities along a gradient of soil pH in Adirondack hardwood forests, ranging from severely acidified (pH 5). In the field, no earthworms were observed where soil pH 4.4 and human dispersal vectors, including proximity to roads and public fishing access, were most prevalent. Overall our results suggest that moderate lime additions can be sufficient to increase earthworm invasion risk where dispersal vectors are present.

  6. Acidification of Earth: An assessment across mechanisms and scales

    Science.gov (United States)

    Rice, Karen; Herman, Janet S.

    2012-01-01

    In this review article, anthropogenic activities that cause acidification of Earth’s air, waters, and soils are examined. Although there are many mechanisms of acidification, the focus is on the major ones, including emissions from combustion of fossil fuels and smelting of ores, mining of coal and metal ores, and application of nitrogen fertilizer to soils, by elucidating the underlying biogeochemical reactions as well as assessing the magnitude of the effects. These widespread activities have resulted in (1) increased CO2concentration in the atmosphere that acidifies the oceans; (2) acidic atmospheric deposition that acidifies soils and bodies of freshwater; (3) acid mine drainage that acidifies bodies of freshwater and groundwaters; and (4) nitrification that acidifies soils. Although natural geochemical reactions of mineral weathering and ion exchange work to buffer acidification, the slow reaction rates or the limited abundance of reactant phases are overwhelmed by the onslaught of anthropogenic acid loading. Relatively recent modifications of resource extraction and usage in some regions of the world have begun to ameliorate local acidification, but expanding use of resources in other regions is causing environmental acidification in previously unnoticed places. World maps of coal consumption, Cu mining and smelting, and N fertilizer application are presented to demonstrate the complex spatial heterogeneity of resource consumption as well as the overlap in acidifying potential derived from distinctly different phenomena. Projected population increase by country over the next four decades indicates areas with the highest potential for acidification, so enabling anticipation and planning to offset or mitigate the deleterious environmental effects associated with these global shifts in the consumption of energy, mineral, and food resources.

  7. A Cumulative Spore Killing Approach: Synergistic Sporicidal Activity of Dilute Peracetic Acid and Ethanol at Low pH Against Clostridium difficile and Bacillus subtilis Spores.

    Science.gov (United States)

    Nerandzic, Michelle M; Sankar C, Thriveen; Setlow, Peter; Donskey, Curtis J

    2016-01-01

    Background.  Alcohol-based hand sanitizers are the primary method of hand hygiene in healthcare settings, but they lack activity against bacterial spores produced by pathogens such as Clostridium difficile and Bacillus anthracis. We previously demonstrated that acidification of ethanol induced rapid sporicidal activity, resulting in ethanol formulations with pH 1.5-2 that were as effective as soap and water washing in reducing levels of C difficile spores on hands. We hypothesized that the addition of dilute peracetic acid (PAA) to acidified ethanol would enhance sporicidal activity while allowing elevation of the pH to a level likely to be well tolerated on skin (ie, >3). Methods.  We tested the efficacy of acidified ethanol solutions alone or in combination with PAA against C difficile and Bacillus subtilis spores in vitro and against nontoxigenic C difficile spores on hands of volunteers. Results.  Acidification of ethanol induced rapid sporicidal activity against C difficile and to a lesser extent B subtilis. The addition of dilute PAA to acidified ethanol resulted in synergistic enhancement of sporicidal activity in a dose-dependent fashion in vitro. On hands, the addition of 1200-2000 ppm PAA enhanced the effectiveness of acidified ethanol formulations, resulting in formulations with pH >3 that were as effective as soap and water washing. Conclusions.  Acidification and the addition of dilute PAA induced rapid sporicidal activity in ethanol. Our findings suggest that it may be feasible to develop effective sporicidal ethanol formulations that are safe and tolerable on skin.

  8. Actual knowledge about some plants with radioprotective effect and about the research of possibilities their use

    International Nuclear Information System (INIS)

    Skalka, J.

    2004-01-01

    Study was undertaken to refer to me importance of radiological protection of human and animal species, especially. After the crash of nuclear power station in Chernobyl, Ukraine, an enormous natural catastrophe. The world wide research programs are dedicated to vearch for plants with radioprotective effect. Apparently representing sea vegetation, brown algae, were considered to have a radioprotective effect just as a large amount of plants representing terrestrial flora. The direction of research programs in the world continues in identification of other unknown plants having radioprotective effect. We would like to direct our research interests in this direction and use enormous possibilities of our rich and various vegetation. (author)

  9. Effect of nitrogen and phosphate limitation on utilization of bitumen ...

    African Journals Online (AJOL)

    PRECIOUS

    2009-12-15

    Dec 15, 2009 ... Carbon (iv) oxide. Bromine + gas. Decolourization. Alkene and alkyne suspect. Cold acidified potassium tetraoxomanganate(vii) + gas. Decolourzation. Alkene and alkyne suspect. Silver trioxonitrate (v) + gas. No reaction. Alkene confirmed. Figure 7. The IR analysis of bitumen before biodegradation.

  10. Effects of sulphur dioxide on the forest ecosystem

    Energy Technology Data Exchange (ETDEWEB)

    Blauel, R A; Malhorta, S S

    1977-01-01

    In situ recovery of oil from sulfur-bearing deposits and subsequent processing to produce petroleum products necessitates the removal, handling, and disposal of sulfur by-products. Due to the nature of sulfur in oil deposits and the processes employed for oil extract, part of the sulfur is converted to SO/sub 2/ and is emitted into the atmosphere. Environmentalist concerns over the emissions are discussed with regard to increasingly acidic precipitation, increasingly greater emissions of sulfur and other acid-forming compounds into the atmosphere, association of the acidity trend with both indigenous and remote sources, deposition of acidifying pollutants on the land in both wet and dry forms, effects of acid precipitation, changes in ecosystems, and long-term effects on the biosphere. A suggested procedure for impact assessment of atmospheric sulfur compounds is presented, employing measurement and determination of environmental effect.

  11. Pharmaceutical pollution of aquatic environment: an emerging and enormous challenge

    Directory of Open Access Journals (Sweden)

    Rzymski Piotr

    2017-06-01

    Full Text Available The global use of pharmaceuticals is on the systematic rise and leads to contamination of surface waters with xenobiotic compounds with a wide range of bioactivity. Waters that receive urban and medical effluents are particularly threatened. The presence of pharmaceuticals in these ecosystems can lead to unpredictable ecological impacts and responses, and may also have an impact on human health. At the same time the identification and quantification of these chemicals, to a large extent remains a subject to scientific investigation than part of a thorough monitoring programme. Their biological effects on aquatic organisms are mainly recognized experimentally and often using concentrations far exceeding environmentally relevant levels. This review paper defines the main sources of pharmaceuticals in the aquatic environment, discusses the fate of these compounds and summarizes the current state-of-the-art of pharmaceutical monitoring in Polish surface waters.

  12. Lactobacillus casei and Lactobacillus fermentum Strains Isolated from Mozzarella Cheese: Probiotic Potential, Safety, Acidifying Kinetic Parameters and Viability under Gastrointestinal Tract Conditions.

    Science.gov (United States)

    de Souza, Bruna Maria Salotti; Borgonovi, Taís Fernanda; Casarotti, Sabrina Neves; Todorov, Svetoslav Dimitrov; Penna, Ana Lúcia Barretto

    2018-03-14

    The objective of this study was to evaluate the probiotic properties of Lactobacillus casei and Lactobacillus fermentum strains, as well as to select novel and safe strains for future development of functional fermented products. The in vitro auto-aggregation, co-aggregation, hydrophobicity, β-galactosidase production, survival to gastrointestinal tract (GIT), and antibiotic susceptibility were evaluated. The selected strains were additionally tested by the presence of genes encoding adhesion, aggregation and colonization, virulence factors, antibiotic resistance, and biogenic amine production, followed by the evaluation of acidifying kinetic parameters in milk, and survival of the strains under simulated GIT conditions during refrigerated storage of fermented milk. Most strains of both species showed high auto-aggregation; some strains showed co-aggregation ability with other lactic acid bacteria (LAB) and/or pathogens, and both species showed low hydrophobicity values. Seven L. casei and six L. fermentum strains produced β-galactosidase enzymes, and ten strains survived well the simulation of the GIT stressful conditions evaluated in vitro. All strains were resistant to vancomycin, and almost all the strains were resistant to kanamycin. L. casei SJRP38 and L. fermentum SJRP43 were distinguished among the other LAB strains by their higher probiotic potential. L. fermentum SJRP43 presented fewer genes related to virulence factors and antibiotic resistance and needed more time to reach the maximum acidification rate (V max ). The other kinetic parameters were similar. Both strains survived well (> 8 log 10 CFU/mL) to the GIT-simulated conditions when incorporated in fermented milk. Therefore, these strains presented promising properties for further applications in fermented functional products.

  13. Acidification of pig slurry effects on ammonia and nitrous oxide emissions, nitrate leaching, and perennial ryegrass regrowth as estimated by N-urea flux

    Directory of Open Access Journals (Sweden)

    Sang Hyun Park

    2018-03-01

    Full Text Available Objective The present study aimed to assess the nitrogen (N use efficiency of acidified pig slurry for regrowth yield and its environmental impacts on perennial ryegrass swards. Methods The pH of digested pig slurry was adjusted to 5.0 or 7.0 by the addition of sulfuric acid and untreated as a control. The pig slurry urea of each treatment was labeled with 15N urea and applied at a rate of 200 kg N/ha immediately after cutting. Soil and herbage samples were collected at 7, 14, and 56 d of regrowth. The flux of pig slurry-N to regrowth yield and soil N mineralization were analyzed, and N losses via NH3, N2O emission and NO3− leaching were also estimated. Results The pH level of the applied slurry did not have a significant effect on herbage yield or N content of herbage at the end of regrowth, whereas the amount of N derived from pig slurry urea (NdfSU was higher in both herbage and soils in pH-controlled plots. The NH4+-N content and the amount of N derived from slurry urea into soil NH4+ fraction (NdfSU-NH4+ was significantly higher in in the pH 5 plot, whereas NO3− and NdfSU-NO3− were lower than in control plots over the entire regrowth period. Nitrification of NH4+-N was delayed in soil amended with acidified slurry. Compared to non-pH-controlled pig slurry (i.e. control plots, application of acidified slurry reduced NH3 emissions by 78.1%, N2O emissions by 78.9% and NO3− leaching by 17.81% over the course of the experiment. Conclusion Our results suggest that pig slurry acidification may represent an effective means of minimizing hazardous environmental impacts without depressing regrowth yield.

  14. Effect of phosphate treatments on microbiological, physicochemical changes of spent hen muscle marinated with Tom Yum paste during chilled storage.

    Science.gov (United States)

    Wongwiwat, Pirinya; Wattanachant, Saowakon; Siripongvutikorn, Sunisa

    2010-06-01

    This research aimed to study the effect of phosphate on quality of ready-to-cook spent hen muscle marinated with Tom Yum paste, a famous Thai food made from chilli, lime leaves and garcinia (pH 2.5-2.9). The effects of phosphate treatments (phosphate types, soaking time, and phosphate concentration) on physical characteristics of spent hen muscle in high acid condition were investigated. Quality changes of muscles pretreated with or without phosphate and marinated with Tom Yum paste were determined during storage at 4 degrees C for 30 days. The acidified muscle pretreated with 40 g L(-1) sodium tripolyphosphate for 10 h had the highest marinade absorption, and the lowest cooking loss and shear force among all treatment samples. Microstructures of acidified muscle pretreated with and without sodium tripolyphosphate showed significant swelling with larger fibre diameter. Phosphate pretreatment had no influence on cooking loss, shear force and thiobarbituric acid reactive substance values of Tom Yum marinated muscle during storage. Tom Yum marination with phosphate pretreatment caused a higher increase in psychrophilic bacteria compared to that of marinating without phosphate. Phosphate pretreatment could not improve the physical quality of Tom-Yum marinated spent hen muscle and affected the antimicrobial property of Tom-Yum marinade, resulting in a reduction of shelf-life of the marinated muscle from 30 days to 20 days. Copyright (c) 2010 Society of Chemical Industry.

  15. Acidic precipitation. Volume 4: Soils, aquatic processes, and lake acidification. Advances in environmental science

    Energy Technology Data Exchange (ETDEWEB)

    Norton, S.A.; Lindberg, S.E.; Page, A.L. (eds.)

    1990-01-01

    Acidic precipitation and its effects have been the focus of intense research for over two decades. Recently, research has focused on a greater understanding of dose-response relationships between atmospheric loading of acidifying material and lake acidity. This volume of the subseries Acidic Precipitation emphasizes acid neutralizing processes and the capacity of terrestrial and aquatic systems to assimilate acidifying substances and, conversely, the ability of systems to recover after acid loading diminishes. Eight chapters have been processed separately for inclusion in the appropriate data bases.

  16. Distributions of 14 elements on 63 absorbers from three simulant solutions (acid-dissolved sludge, acidified supernate, and alkaline supernate) for Hanford HLW Tank 102-SY

    International Nuclear Information System (INIS)

    Marsh, S.F.; Svitra, Z.V.; Bowen, S.M.

    1994-08-01

    As part of the Hanford Tank Waste Remediation System program at Los Alamos, we evaluated 63 commercially available or experimental absorber materials for their ability to remove hazardous components from high-level waste (HLW). These absorbers included cation and anion exchange resins, inorganic exchangers, composite absorbers, and a series of liquid extractants sorbed on porous support-beads. We tested these absorbers with three solutions prepared to simulate acid-dissolved sludge (pH 0.6), acidified supernate (pH 3.5), and alkaline supernate (pH 13.9) from underground storage tank 102-SY at the Hanford Reservation near Richland, Washington. To these simulants we added the appropriate radionuclides and used gamma spectrometry to measure fission products (Ce, Cs, Sr, Tc, and Y), actinides (U, Pu, and Am), and matrix elements (Cr, Co, Fe, Mn, Zn, and Zr). For each of more than 2500 element/absorber/solution combinations, we measured distribution coefficients for dynamic contact periods of 30 min, 2 h, and 6 h to obtain information about sorption kinetics. Because we measured the sorption of many different elements, the tabulated results indicate those elements most likely to interfere with the sorption of elements of greater interest. On the basis of nearly 7500 measured distribution coefficients, we determined that many of these absorbers appear suitable for processing HLW. This study supersedes the previous version of LA-12654, in which results attributed to a solution identified as an alkaline supernate simulant were misleading because that solution contained insufficient hydroxide

  17. The endogenous alkaloid harmane: acidifying and activity-reducing effects on hippocampal neurons in vitro.

    Science.gov (United States)

    Bonnet, Udo; Scherbaum, Norbert; Wiemann, Martin

    2008-02-15

    The endogenous alkaloid harmane is enriched in plasma of patients with neurodegenerative or addictive disorders. As harmane affects neuronal activity and viability and because both parameters are strongly influenced by intracellular pH (pH(i)), we tested whether effects of harmane are correlated with altered pH(i) regulation. Pyramidal neurons in the CA3 field of hippocampal slices were investigated under bicarbonate-buffered conditions. Harmane (50 and 100 microM) reversibly decreased spontaneous firing of action potentials and caffeine-induced bursting of CA3 neurons. In parallel experiments, 50 and 100 microM harmane evoked a neuronal acidification of 0.12+/-0.08 and 0.18+/-0.07 pH units, respectively. Recovery from intracellular acidification subsequent to an ammonium prepulse was also impaired, suggesting an inhibition of transmembrane acid extrusion by harmane. Harmane may modulate neuronal functions via altered pH(i)-regulation. Implications of these findings for neuronal survival are discussed.

  18. Antifungal effect of kefir fermented milk and shelf life improvement of corn arepas.

    Science.gov (United States)

    Gamba, Raúl Ricardo; Caro, Carlos Andrés; Martínez, Olga Lucía; Moretti, Ana Florencia; Giannuzzi, Leda; De Antoni, Graciela Liliana; León Peláez, Angela

    2016-10-17

    Fungal contamination negatively affects the production of cereal foods such as arepa loaf, an ancient corn bread consumed daily in several countries of Latin-America. Chemical preservatives such as potassium sorbate are applied in order to improve the arepa's shelf life and to reduce the health risks. The use of natural preservatives such as natural fermented products in food commodities is a common demand among the consumers. Kefir is a milk fermented beverage obtained by fermentation of kefir grains. Its antibacterial and probiotic activity has been exhaustively demonstrated. Our objectives were to determine the antifungal effect of kefir fermented milk on Aspergillus flavus AFUNL5 in vitro and to study if the addition of kefir fermented milk to arepas could produce shelf life improvement. We determined the antifungal effect on solid medium of kefir cell-free supernatants (CFS) obtained under different fermentation conditions. Additionally, we compared the antifungal effect of kefir CFS with that obtained with unfermented milk artificially acidified with lactic plus acetic acids (lactic and acetic acids at the same concentration determined in kefir CFS) or with hydrochloric acid. Finally, kefir was added to the corn products either in the loaf recipe (kefir-baked arepas) or sprayed onto the baked-loaf surface (kefir-sprayed arepas). The loaves' resistance to natural and artificial fungal contamination and their organoleptic profiles were studied. The highest fungal inhibition on solid medium was achieved with kefir CFS produced by kefir grains CIDCA AGK1 at 100 g/L, incubated at 30 °C and fermented until pH 3.3. Other CFS obtained from different fermentation conditions achieved less antifungal activity than that mentioned above. However, CFS of milk fermented with kefir grains, until pH 4.5 caused an increase of growth rates. Additionally, CFS produced by kefir grains CIDCA AGK1 at 100 g/L, incubated at 30 °C and fermented until pH 3.3 achieved higher

  19. Enormous periodic doppler shifts in SS 433

    International Nuclear Information System (INIS)

    Margon, B.; Ford, H.C.; Grandi, S.A.; Stone, R.P.S.

    1979-01-01

    We have previously reported prominent ''moving' emission lines in the visible spectrum of Stephenson-Sanduleak 433, the optical counterpart of a variable radio and X-ray source. Further observations show that despite the implausible velocities and changes in velocities implied if the moving features are interpreted as Doppler-shifted Balmer lines, this explanation is indeed correct. Spectroscopy of SS 433 on 51 mights in 1978--1979 reveals that the unidentified features are two sets of Balmer and He I lines, one with large and changing redshift, and the other with large and changing blueshift. Combining our data with published earlier observations, we obtain Doppler shifts on 80 nights in the period 1978 June to 1979 June. These data indicate that the velocity variations are cyclical, repeating in both the blueshift and redshift systems with a period of 164 +- 3 days. The two systems have thus far been observed to reach maximum positive and negative radial velocities of +50,000 and -35,000 km s -1 , respectively, are always symmetric about redshift z=0.04, and follow roughly sinusoidal velocity curves. We discuss in addition a variety of interesting short-term spectroscopic details, including minor but highly significant deviations of the radial velocity from the sinusoid, and nightly line profile changes, sometimes appearing as mirror-image events in the redshift and blueshift systems. The behavior of SS 433 is unprecedented

  20. At math meetings, enormous theorem eclipses fermat.

    Science.gov (United States)

    Cipra, B

    1995-02-10

    Hardly a word was said about Fermat's Last Theorem at the joint meetings of the American Mathematical Society and the Mathematical Association of America, held this year from 4 to 7 January in San Francisco. For Andrew Wiles's proof, no news is good news: There are no reports of mistakes. But mathematicians found plenty of other topics to discuss. Among them: a computational breakthrough in the study of turbulent diffusion and progress in slimming down the proof of an important result in group theory, whose original size makes checking the proof of Fermat's Last Theorem look like an afternoon's pastime.

  1. Greenhouse effect: Myth or reality

    International Nuclear Information System (INIS)

    Martin, J.L.

    1992-01-01

    This paper debates on greenhouse effect controversy. Natural greenhouse effect is beneficent but additional greenhouse effect, in relation with human activities, can present a major risk for humanity. However an international agreement is difficult owing to the enormous costs which could not be endured by South economies. A tax on carbon dioxide emissions would have for consequence a wave of industrial delocalizations without precedent with important unemployment in Europe and no impact on additional greenhouse effect because it is a radiative effect and it is not a classic local chemical pollution. 11 refs., 10 figs

  2. Valor nutricional e viabilidade econômica de rações suplementadas com maltodextrina e acidificante para leitões desmamados Nutritional value and economical viability of diets supplemented with maltodextrin and acid mix for weanling pigs

    Directory of Open Access Journals (Sweden)

    Anália Maria Ribeiro da Silva

    2008-02-01

    Full Text Available Para avaliar os efeitos da suplementação dietética de maltodextrina em substituição parcial à lactose e de acidificante sobre o desempenho, a digestibilidade e a viabilidade econômica em leitões desmamados, foram utilizados 144 leitões Dalland com pesos iniciais de 6,0 ± 0,45 kg no ensaio 1, no qual foram utilizadas rações mais complexas, e de 5,8 ± 0,53 kg no ensaio 2. Os delineamentos experimentais foram em blocos ao acaso, com arranjo fatorial 2 × 2 dos tratamentos (ausência ou presença de maltodextrina × ausência ou presença de acidificante. Não houve interação maltodextrina × acidificante e efeito do acidificante sobre o desempenho dos leitões em ambos os ensaios. A maltodextrina determinou maior consumo diário de ração e ganho diário de peso de 0 a 14 dias no ensaio 1, mas não influenciou o desempenho na fase de 0 a 28 dias e nos períodos de 0 a 16 e de 0 a 30 dias do ensaio 2. Não houve interação maltodextrina ´ acidificante nem efeitos desses fatores sobre os coeficientes de digestibilidade aparente dos nutrientes das rações pré-iniciais do ensaio 2. As rações com maltodextrina e maltodextrina + acidificante proporcionaram melhor resultado econômico. A maltodextrina e o acidificante não influenciaram o valor nutricional das rações. A maltodextrina constitui alternativa às fontes de lactose para leitões desmamados.To evaluate the effects of the dietary supplementation of maltodextrin in partial replacement of lactose and acidifiers on performance, digestibility and economical viability of weanling pigs, 144 Dalland piglets with initial weights of 6.0 ± 0.45 kg were used in trial 1, where more complex diets were used, and 5.8 ± 0.53 kg in trial 2. A completely randomized block design in a 2 × 2 factorial arrangement was used for the treatments (absence or presence of maltodextrin ´ absence or presence of acidifiers. There was no maltodextrin ´ acidifiers interaction or acidifiers effects on

  3. Cost effectiveness of withdrawal of fall-risk-increasing drugs in geriatric outpatients

    NARCIS (Netherlands)

    van der Velde, Nathalie; Meerding, Willen Jan; Looman, Caspar W.; Pols, Huibert A. P.; van der Cammen, Tischa J. M.

    2008-01-01

    BACKGROUND: Withdrawal of fall-risk-increasing drugs has been proven to be effective in older persons. However, given the enormous rise in healthcare costs in recent decades, the effect of such withdrawals on healthcare costs also needs to be considered. METHOD: Within a common geriatric outpatient

  4. The effect of ethylenediaminetetraacetic acid on heat resistance and recovery of Clostridium sporogenes PA 3679 spores treated in HTST conditions.

    Science.gov (United States)

    Silla Santos, M H; Torres Zarzo, J

    1997-03-03

    The effect of ethylenediaminetetraacetic acid (EDTA) on the heat resistance of Clostridium sporogenes PA 3679 spores was studied. EDTA was added to heating substrates and recovery media in order to establish which stage of the heat treatment registered the greatest EDTA activity. The heating substrates assayed were phosphate buffer (pH 7.0) and white asparagus purée, at natural pH (5.8) and acidified with citric acid and glucono-delta-lactone (GDL) to pH 5.5, 5.0 and 4.5. Recovery of survivors was carried out in MPA3679A medium in various conditions of acidification with citric and GDL (250 and 500 ppm), at pH 7.5 6.5 and 6.0. The results show greater activity of EDTA on spores when it was applied in recovery of heat injured spores, than during heating. The strongest influence of EDTA during heating was found in phosphate buffer (pH 7.0), with the effect being most evident at 121 and 126 degrees C, and in asparagus purée, at 121 degrees C and pH 5.8 rather than acidified. In recovery, the inhibiting activity of EDTA was more evident in spores subjected to more severe heat treatment, either by increasing the exposure time or by raising the temperature to 130 or 135 degrees C. The pH level of the recovery medium also affected the antimicrobial activity of EDTA, which had a greater inhibiting effect at pH 7.5 than at lower pH levels (6.5, 6.0).

  5. Evaluation of citric acid and GDL in the recovery at different pH levels of Clostridium sporogenes PA 3679 spores subjected to HTST treatment conditions.

    Science.gov (United States)

    Silla Santos, M H; Torres Zarzo, J

    1996-04-01

    Spores of Clostridium sporogenes PA 3679 were treated at different temperatures (121, 126, 130 and 135 degrees C) in white asparagus purée (pH 5.8) and acidified with glucono-delta-lactone (GDL) and citric acid to pH levels of 5.5, 5.0 and 4.5. Afterwards, the spores were recovered in MPA3679 medium in various conditions: unacidified (pH 7.5), acidified with GDL (500 ppm) and acidified with citric acid (500 and 250 ppm) to pH levels of 6.5, 6.0 and 5.0. The results indicated that the pH levels, concentration and type of acid used act synergistically rather than independently. Citric acid has a stronger inhibiting effect than GDL on the recovery of C. sporogenes PA 3679 spores. At the higher heat treatments (130 and 135 degrees C) the major injury on the spores sensitize more than against the acids and low pH values.

  6. Effect of dissolved oxygen on redox potential and milk acidification by lactic acid bacteria isolated from a DL-starter culture

    DEFF Research Database (Denmark)

    Larsen, Nadja; Werner, Birgit Brøsted; Vogensen, Finn Kvist

    2015-01-01

    potential to negative values. Kinetic parameters of the DL-starter culture were comparable with the best acidifying and reducing strains, indicating their additive effects. Acidification curves were mostly diauxic at all oxygen levels, displaying 2 maxima of acidification rate: before (aerobic maximum...... subspecies in DL-starter cultures. This knowledge is important for dairies to ensure optimized, fast, and controlled milk fermentations, leading to greater standardization of dairy products.......Milk acidification by DL-starter cultures [cultures containing Lactococcus lactis diacetylactis (D) and Leuconostoc (L) species] depends on the oxidation-reduction (redox) potential in milk; however, the mechanisms behind this effect are not completely clear. The objective of this study...

  7. On operator effectiveness: The role of expertise and familiarity of environment on the detection of deviant behaviour

    NARCIS (Netherlands)

    Wijn, R.; Berg, H. van den; Lousberg, M.

    2013-01-01

    This paper examines factors contributing to the effectiveness of camera operators in urban camera surveillance. The use of camera surveillance has taken an enormous flight in the past decades. Despite this increase, its effectiveness is strongly debated. One reason for the disputed effectiveness may

  8. Effect of sucralfate on gastric permeability in an ex vivo model of stress-related mucosal disease in dogs.

    Science.gov (United States)

    Hill, Tracy L; Lascelles, B Duncan X; Blikslager, Anthony T

    2018-03-01

    Sucralfate is a gastroprotectant with no known systemic effects. The efficacy of sucralfate for prevention and treatment of stress-related mucosal diseases (SRMD) in dogs is unknown. To develop a canine ex vivo model of SRMD and to determine the effect of sucralfate on mucosal barrier function in this model. Gastric antral mucosa was collected immediately postmortem from 29 random-source apparently healthy dogs euthanized at a local animal control facility. Randomized experimental trial. Sucralfate (100 mg/mL) was applied to ex vivo canine gastric mucosa concurrent with and after acid injury. Barrier function was assessed by measurement of transepithelial electrical resistance (TER) and radiolabeled mannitol flux. Application of acidified Ringers solution to the mucosal side of gastric antrum caused a reduction in gastric barrier function, and washout of acidified Ringers solution allowed recovery of barrier function (TER: 34.0 ± 2.8% of control at maximum injury, 71.3 ± 5.5% at recovery, P < .001). Sucralfate application at the time of injury or after injury significantly hastened recovery of barrier function (TER: 118.0 ± 15.2% of control at maximum injury, P < .001 and 111.0 ± 15.5% at recovery, P = .35). Sucralfate appeared effective at restoring defects in gastric barrier function induced by acid and accelerating repair of tissues subjected to acid in this model, suggesting that sucralfate could have utility for the treatment and prevention of SRMD in dogs. Copyright © 2018 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.

  9. The complex effects of ocean acidification on the prominent N2-fixing cyanobacterium Trichodesmium.

    Science.gov (United States)

    Hong, Haizheng; Shen, Rong; Zhang, Futing; Wen, Zuozhu; Chang, Siwei; Lin, Wenfang; Kranz, Sven A; Luo, Ya-Wei; Kao, Shuh-Ji; Morel, François M M; Shi, Dalin

    2017-05-05

    Acidification of seawater caused by anthropogenic carbon dioxide (CO 2 ) is anticipated to influence the growth of dinitrogen (N 2 )-fixing phytoplankton, which contribute a large fraction of primary production in the tropical and subtropical ocean. We found that growth and N 2 -fixation of the ubiquitous cyanobacterium Trichodesmium decreased under acidified conditions, notwithstanding a beneficial effect of high CO 2 Acidification resulted in low cytosolic pH and reduced N 2 -fixation rates despite elevated nitrogenase concentrations. Low cytosolic pH required increased proton pumping across the thylakoid membrane and elevated adenosine triphosphate production. These requirements were not satisfied under field or experimental iron-limiting conditions, which greatly amplified the negative effect of acidification. Copyright © 2017, American Association for the Advancement of Science.

  10. Study on combined effects of acidification and sonication on selected quality attributes of carrot juice during storage

    International Nuclear Information System (INIS)

    Jabbar, S.; Hu, B.; Ali, S.

    2014-01-01

    This study evaluated the combined effects of acid blanching and sonication treatments on selected quality parameters of carrot juice stored at 4 degree C for 18 days. Carrots were blanched in acidified water (40g/L citric acid) at 100 degree C for 4 min and the juice was then extracted. Sonication of the juice was done at an amplitude level of 70% and a frequency of 20 kHz for 2 min at 15 degree C, keeping the pulse duration of 5 Sec on and 5 Sec off. As results, the combined treatment of acidification and sonication of carrot juice showed a significant decrease in pH and increase (P < 0.05) in acidity which remained stable during storage period. No significant changes were observed in Brix. Color values (L, a, b) and non enzymatic browning (NEB) influenced significantly in acidified and sonicated carrot juice during storage period. Maximum stability of total phenol, total antioxidant capacity, cloud value and ascorbic acid were also observed in the combined treatment of acidification and sonication. The findings of this study indicated that the combined treatments of acidification and sonication may successfully be utilized for the production of high quality carrot juice with improved stability of total phenol, total antioxidant capacity, cloud value and ascorbic acid during 18 days of storage. (author)

  11. Effects of sulfur and nitrogen on nutrients uptake of corn using ...

    African Journals Online (AJOL)

    N) fertilizer using acidified water (pH 6.5) to determine nutrients uptake and growth of maize plants grown in calcareous sandy soil. Four levels of elemental sulfur (0, 1, 5 and 10 t ha-1), two levels of N (0 and 0.34 t N ha-1) were tested at Al ...

  12. ORGANIC MATTER AND HUMIC FRACTIONS OF A HAPLIC ACRISOL AS AFFECTED BY COMPOSTED PIG SLURRY

    Directory of Open Access Journals (Sweden)

    Ana Cristina Lüdtke

    2016-01-01

    Full Text Available The goal of this study was to investigate the effect of composted pig slurry (PS on the organic matter concentration and distribution of humic acid (HA, fulvic acid (FA and humin (HU fractions. The fractions were quantified following the addition of composted PS to the soil, which was produced with no acidification (T2 or with acidification with H3PO4 (T3; and in soil without compost addition (T1. The HA chemical composition was analyzed by FTIR spectroscopy. The addition of the two composts did not change the soil carbon concentration but affected the distribution of the humic fractions. For the three treatments, the carbon concentration of humic substances increased until 52 days following compost addition, with more pronounced increases with the addition of non-acidified PS compost (14.5 g kg-1 and acidified PS compost (15.1 g kg-1. This increase was reflected in both the FA and HA concentrations. The addition of compost with PS acidification resulted in the formation of larger humic micelles (HA with higher aromatic content and fewer functional groups than the non-acidified PS compost. These findings, together with a lower proportion of carbohydrate-type structures, indicated the presence of more stable humic micelles in the soil treated with acidified PS compost.

  13. Shape memory effect alloys

    International Nuclear Information System (INIS)

    Koshimizu, S.

    1992-01-01

    Although the pseudo- or super-elasticity phenomena and the shape memory effect were known since the 1940's, the enormous curiosity and the great interest to their practical applications emerged with the development of the NITINOL alloy (Nickel-Titanium Naval Ordance Laboratory) by the NASA during the 1960's. This fact marked the appearance of a new class of materials, popularly known as shape memory effect alloys (SMEA). The objective of this work is to present a state-of-the-art of the development and applications for the SMEA. (E.O.)

  14. Alternative leaching processes for uranium ores

    International Nuclear Information System (INIS)

    Ring, R.J.

    1979-01-01

    Laboratory studies have been carried out to compare the extraction of uranium from Australian ores by conventional leaching in sulphuric acid with that obtained using hydrochloric acid and acidified ferric sulphate solutions. Leaching with hydrochloric acid achieved higher extractions of radium-226 but the extraction of uranium was reduced considerably. The use of acidified ferric sulphate solution reduced acid consumption by 20-40% without any detrimental effect on uranium extraction. The ferric ion, which is reduced during leaching, can be reoxidized and recycled after the addition of acid makeup. Hydrogen peroxide was found to be an effective oxidant in conventional sulphuric acid leaching. It is more expensive than alternative oxidants, but it is non-polluting, lesser quantities are required and acid consumption is reduced

  15. Reduced phosphorus retention by anoxic bottom sediments after the remediation of an industrial acidified lake area: Indications from P, Al, and Fe sediment fractions.

    Science.gov (United States)

    Nürnberg, Gertrud K; Fischer, Rachele; Paterson, Andrew M

    2018-06-01

    Formerly acidified lakes and watersheds can become more productive when recovering from acidity, especially when exposed to anthropogenic disturbance and increased nutrient loading. Occasional toxic cyanobacterial blooms and other signs of eutrophication have been observed for a decade in lakes located in the Sudbury, Ontario, mining area that was severely affected by acid deposition before the start of smelter emission reductions in the 1970s. Oligotrophic Long Lake and its upstream lakes have been exposed to waste water input and development impacts from the City of Greater Sudbury and likely have a legacy of nutrient enrichment in their sediment. Based on observations from other published studies, we hypothesized that P, which was previously adsorbed by metals liberated during acidification caused by the mining activities, is now being released from the sediment as internal P loading contributing to increased cyanobacteria biomass. Support for this hypothesis includes (1) lake observations of oxygen depletion and hypolimnetic anoxia and slightly elevated hypolimnetic total P concentration and (2) P, Al, and Fe fractionation of two sediment layers (0-5, 5-10 cm), showing elevated concentrations of TP and iron releasable P (BD-fraction), decreased concentrations in fractions associated with Al, and fraction ratios indicating decreased sediment adsorption capacity. The comparison with two moderately enriched lakes within 200 km distance, but never directly affected by mining operations, supports the increasing similarity of Long Lake surficial sediment adsorption capacity with that of unaffected lakes. There is cause for concern that increased eutrophication including the proliferation of cyanobacteria of formerly acidic lakes is wide-spread and occurs wherever recovery coincides with anthropogenic disturbances and physical changes related to climate change. Copyright © 2018 Elsevier B.V. All rights reserved.

  16. Effect of type of suckling and polyunsaturated fatty acid use on lamb production. 1. Productive performances and quanti-qualitative characteristics of the carcass

    Directory of Open Access Journals (Sweden)

    Francesco Bozzo

    2010-01-01

    Full Text Available The aim of the study was to estimate the influence of artificial rearing and of the addition of polyunsaturated fatty acids(PUFA to an acidified milk replacer on the productive performances and on the quanti-qualitative characteristics of thecarcass. Fifty one twin-born Gentile di Puglia lambs were subdivided into three homogenous groups (9 females and 8males assigned the following feeding treatments: maternal milk (MM; acidified milk replacer (MR; acidified milk replacer+ 10 ml/l of a mixture of linseed and fish oil rich in PUFA (MR+PUFA.Milk consumptions and live weights were recorded weekly. Seven males from each group were slaughtered at 45 days ofage and the net warm dressing percentage, the composition of the carcass and the colorimetric characteristics of theLongissimus lumborum muscle were estimated. Artificial rearing improved the daily weight gain (0.182-0.172 vs 0.128Kg; PThe addition of omega-3 fatty acids to the diet did not influence the weight gain, the feed conversion index, the net warmdressing percentage and the incidence of the different meat cuts of the half carcass. However, it significantly increasedthe half carcass length (37.86 vs 35.0 cm; Pto the MR group reduced the proportion of lean (63.21 vs 53.98 %; PP<0.01 in the lumbar region.

  17. Effects of Students' Characteristics on Online Learning Readiness: A Vocational College Example

    Science.gov (United States)

    Cigdam, Harun; Yildirim, Osman Gazi

    2014-01-01

    Educational institutions rapidly adopt concepts and practices of online learning systems for students. But many institutions' online learning programs face enormous difficulty in achieving successful strategies. It is essential to evaluate its different aspects and understand factors which influence its effectiveness. Readiness stands out among…

  18. Social Consumer Neuroscience: Neurophysiological Measures of Advertising Effectiveness in a Social Context

    NARCIS (Netherlands)

    R. Pozharliev (Rumen); W.J.M.I. Verbeke (Willem); R.P. Bagozzi (Richard)

    2017-01-01

    textabstractThe application of neurophysiological methods to study the effects of advertising on consumer purchase behavior has seen an enormous growth in recent years. However, little is known about the role social settings have on shaping the human brain during the processing of advertising

  19. Actual knowledge about some plants with radioprotective effect and about the research of possibilities their use; Aktualne poznatky o niektorych rastlinach s radioprotektivnym ucinkom a o vyskume moznosti ich vyuzitia

    Energy Technology Data Exchange (ETDEWEB)

    Skalka, J [I. Interna klinika, Univerzita veterinarsneho lekarstva, Kosice (Slovakia)

    2004-06-15

    Study was undertaken to refer to me importance of radiological protection of human and animal species, especially. After the crash of nuclear power station in Chernobyl, Ukraine, an enormous natural catastrophe. The world wide research programs are dedicated to vearch for plants with radioprotective effect. Apparently representing sea vegetation, brown algae, were considered to have a radioprotective effect just as a large amount of plants representing terrestrial flora. The direction of research programs in the world continues in identification of other unknown plants having radioprotective effect. We would like to direct our research interests in this direction and use enormous possibilities of our rich and various vegetation. (author)

  20. Effectiveness of a temperature control system in home induction hobs to reduce acrylamide formation during pan frying

    DEFF Research Database (Denmark)

    Guillen, S.; Oria, R.; Salvador, M. L.

    2017-01-01

    Three trials were conducted to determine the influence of the use of temperature control systems on physico-chemical characteristics and acrylamide formation in the domestic preparation of potatoes. French fries were pre-treated by soaking in water or acidified water, and then they were cooked...

  1. Effect factors for terrestrial acidification in Brazil

    DEFF Research Database (Denmark)

    Crespo Mendes, Natalia; Laurent, Alexis; Hauschild, Michael Zwicky

    conditions, which is an essential approach considering countries like Brazil, with high biodiversity. Previous studies have assessed the impacts of terrestrial acidification from the estimations of the potential losses of vascular plants species richness as a result of exposure to acidifying substances...... for 13 biomes, with 2409 species addressed for whole world. In this context this work aims to provide spatially-differentiated effect factors (EF) for terrestrial acidification in Brazil and support the development of spatially-differentiated characterization factors for Brazil. In order to maintain...... in Brazil, represented by 33167 species, indicating that this is a comprehensive study. Maps of soil pH in Brazil were extracted at 1-km resolution and pH values were extracted for the depth range of 0-30cm. For each ecoregion, species richness was plotted against soil pH and the exposure-response curves...

  2. Effect of type of suckling and polyunsaturated fatty acid use on lamb production. 2. Chemical and fatty acid composition of raw and cooked meat

    Directory of Open Access Journals (Sweden)

    Francesco Toteda

    2010-01-01

    Full Text Available This study was carried out in order to examine the chemical and fatty acid composition of raw and cooked meat obtained fromlambs raised under mothers or reared by artificial suckling with acidified milk replacers with or without polyunsaturated fattyacid (PUFA supplementation. Meat samples were taken from twenty Gentile di Puglia male lambs subjected to the followingfeeding treatments: the control group received only maternal milk (MM, n.=6 while two groups were reared by artificial sucklingwith an acidified milk replacer (MR, n.=7 or with an acidified milk replacer supplemented with 10 ml/l of a PUFA enrichedoil (MR+PUFA, n.=7. Lambs were slaughtered at 45 days of age. After 24 hours of refrigeration at 4 °C, the lumbar regionwas dissected from each right half-carcass and split into pieces, one of which was used raw while the other was cooked in aventilated electric oven at 180 °C until an internal temperature of 75 °C was reached. Chemical and fatty acid analysis wereperformed on raw and cooked meat, while only raw meat was assessed for cholesterol. Cooking losses were also evaluated.Meat obtained from MR+PUFA fed lambs contained more fat (Punder mothers increased the total amount of saturated fatty acids (SFA, compared with both the MR group (Pthe MR+PUFA one (Pcomparison with both MR diets. The highest PUFA/SFA ratio of meat was recorded for the MR+PUFA group (0.27, with statisticaldifferences respect to the MR group (0.21; Pmilk produced meat containing more cholesterol than the MR+PUFA group (85.89 vs 76.26 mg/100 g; Pindex of meat was higher following natural rearing in comparison with the MR+PUFA treatment (1.34 vs 1.05;Pand 0.76, respectively; Pparameters evaluated. In conclusion, artificial suckling with acidified milk replacers improves some meat quality features.Supplementation of milk replacers with PUFAs, although in a limited way, may improve the dietetic properties of lamb meat.

  3. Quantifying geomorphic change and characterizing uncertainty in repeat aerial lidar over an enormous area: Blue Earth County, MN

    Science.gov (United States)

    Schaffrath, K. R.; Belmont, P.; Wheaton, J. M.

    2013-12-01

    High-resolution topography data (lidar) are being collected over increasingly larger geographic areas. These data contain an immense amount of information regarding the topography of bare-earth and vegetated surfaces. Repeat lidar data (collected at multiple times for the same location) enables extraction of an unprecedented level of detailed information about landscape form and function and provides an opportunity to quantify volumetric change and identify hot spots of erosion and deposition. However, significant technological and scientific challenges remain in the analysis of repeat lidar data over enormous areas (>1000 square kilometers), not the least of which involves robust quantification of uncertainty. Excessive sedimentation has been documented in the Minnesota River and many reaches of the mainstem and tributaries are listed as impaired for turbidity and eutrophication under the Clean Water Act of 1972. The Blue Earth River and its tributaries (Greater Blue Earth basin) have been identified as one of the main sources of sediment to the Minnesota River. Much of the Greater Blue Earth basin is located in Blue Earth County (1,982 square kilometers) where airborne lidar data were collected in 2005 and 2012, with average bare-earth point densities of 1 point per square meter and closer to 2 points per square meter, respectively. One of the largest floods on record (100-year recurrence interval) occurred in September 2010. A sediment budget for the Greater Blue Earth basin is being developed to inform strategies to reduce current sediment loads and better predict how the basin may respond to changing climate and management practices. Here we evaluate the geomorphic changes that occurred between 2005 and 2012 to identify hotspots of erosion and deposition, and to quantify some of the terms in the sediment budget. To make meaningful interpretations of the differences between the 2005 and 2012 lidar digital elevation models (DEMs), total uncertainty must be

  4. Effectiveness of turf stripping as a measure for restoring species-rich fen meadows in suboptimal hydrological conditions

    NARCIS (Netherlands)

    Hoek, van der D.; Heijmans, M.M.P.D.

    2007-01-01

    Most species-rich fen meadows in nature reserves in The Netherlands are acidified due to weaker upwelling of base-rich groundwater. The present study investigated whether and why turf stripping combined with superficial drainage might promote the long-term recovery of such meadows and restore the

  5. Effect of Different Solvents on the Measurement of Phenolics and the Antioxidant Activity of Mulberry (Morus atropurpurea Roxb.) with Accelerated Solvent Extraction.

    Science.gov (United States)

    Yang, Jiufang; Ou, XiaoQun; Zhang, Xiaoxu; Zhou, ZiYing; Ma, LiYan

    2017-03-01

    The effects of 9 different solvents on the measurement of the total phenolics and antioxidant activities of mulberry fruits were studied using accelerated solvent extraction (ASE). Sixteen to 22 types of phenolics (flavonols, flavan-3-ols, flavanol, hydroxycinnamic acids, hydroxybenzoic acids, and stilbenes) from different mulberry extracts were characterized and quantified using HPLC-MS/MS. The principal component analysis (PCA) was used to determine the suitable solvents to distinguish between different classes of phenolics. Additionally, the phenolic extraction abilities of ASE and ultrasound-assisted extraction (UAE) were compared. The highest extraction efficiency could be achieved by using 50% acidified methanol (50MA) as ASE solvents with 15.14 mg/gallic acid equivalents g dry weight of mulberry fruit. The PCA results revealed that the 50MA followed by 50% acidified acetone (50AA) was the most efficient solvent for the extraction of phenolics, particularly flavonols (627.12 and 510.31 μg/g dry weight, respectively), while water (W) was not beneficial to the extraction of all categories of phenolics. Besides, the results of 3 antioxidant capability assays (DPPH, ABTS free radical-scavenging assay, and ferric-reducing antioxidant power assay) showed that water-based organic solvents increased the antioxidant capabilities of the extracts compared with water or pure organic solvents. ASE was more suitable for the extraction of phenolics than UAE. © 2017 Institute of Food Technologists®.

  6. Effect of dextran and dextran sulfate on the structural and rheological properties of model acid milk gels.

    Science.gov (United States)

    Pachekrepapol, U; Horne, D S; Lucey, J A

    2015-05-01

    Various types of polysaccharides are widely used in cultured dairy products. However, the interaction mechanisms, between milk proteins and these polysaccharides, are not entirely clear. To explore the interactions between uncharged and charged polysaccharides and the caseins, we used a model acid-milk-gel system, which allowed acidification to occur separately from gelation. The effect of adding uncharged dextran (DX; molecular weight ~2.0×10(6) Da) and negatively charged dextran sulfate (DS; molecular weight ~1.4×10(6) Da) to model acid milk gels was studied. Two concentrations (0.075 and 0.5%, wt/wt) of DX or DS were added to cold milk (~0°C) that had been acidified to pH values 4.4, 4.6, 4.8, or 4.9. Acidified milks containing DX or DS were then quiescently heated at the rate of 0.5°C/min to 30°C, which induced gelation, and gels were then held at 30°C for 17 h to facilitate gel development. Dynamic small-amplitude-oscillation rheology and large-deformation (shear) tests were performed. Microstructure of gels was examined by fluorescence microscopy. Gels made with a high concentration of DX gelled at a lower temperature, but after 17 h at 30°C, these gels exhibited lower storage moduli and lower yield-stress values. At pH 4.8 or 4.9 (pH values greater than the isoelectric point of caseins), addition of 0.5% DS to acidified milk resulted in lower gelation temperature. At pH 4.4 (pH values less than the isoelectric point of caseins), addition of 0.5% DS to acidified milk resulted in gels with very high stiffness values. Gels made at pH 4.8 or 4.9 with both concentrations of DS had much lower stiffness and yield-stress values than control gels. Microstructural analysis indicated that gels made at pH 4.4 with the addition of 0.5% DX exhibited large protein strands and pores, whereas gels made with 0.075% DX or the control gels had a finer protein matrix. At higher pH values (>4.4), gels made with 0.5% DX had a finer structure. At all pH values, gels made

  7. Emissions of ammonia, carbon dioxide, and hydrogen sulfide from swine wastewater during and after acidification treatment: effect of pH, mixing and aeration

    DEFF Research Database (Denmark)

    Dai, Xiao-Rong; Blanes-Vidal, Victoria

    2013-01-01

    setup consisting of nine dynamic flux chambers. Three pH levels (pH = 6.0, pH = 5.8 and pH = 5.5), combined with short-term aeration and venting (with an inert gas) treatments were studied. Acidification reduced average NH(3) emissions from swine slurry stored after acidification treatment compared...... to emissions during storage of non-acidified slurry. The reduction were 50%, 62% and 77% when pH was reduce to 6.0, 5.8 and 5.5, respectively. However, it had no significant effect on average CO(2) and H(2)S emissions during storage of slurry after acidification. Aeration of the slurry for 30 min had no effect...

  8. Social Consumer Neuroscience: Neurophysiological Measures of Advertising Effectiveness in a Social Context

    OpenAIRE

    Pozharliev, Rumen; Verbeke, Willem; Bagozzi, Richard

    2017-01-01

    textabstractThe application of neurophysiological methods to study the effects of advertising on consumer purchase behavior has seen an enormous growth in recent years. However, little is known about the role social settings have on shaping the human brain during the processing of advertising stimuli. To address this issue, we first review previous key findings of neuroscience research on advertising effectiveness. Next, we discuss traditional advertising research into the effects social cont...

  9. A review on acidifying treatments for vegetable canned food.

    Science.gov (United States)

    Derossi, A; Fiore, A G; De Pilli, T; Severini, C

    2011-12-01

    As is well known, pasteurization treatments are not sufficient for destroying heat resistance of spore forming microorganisms, which are prevented from germination and growing by pH reducing. So, the acidification process becomes one of the most important pre-treatments for the canning industry. It is commonly applied before pasteurization treatment with the purpose of inhibiting spore germination and for reducing heat resistance of the microorganism, thereby allowing to reduce the time or temperature values of the heat treatment. With the aim to reduce the pH of vegetables several techniques are available but their application is not easy to plan. Often, industries define operative conditions only on the basis of empirical experience, thus increasing the risk of microbial growth or imparting an unpleasant sour taste. With the aim of highlighting the correct plan and management of acidification treatments to reach safety without degrading quality of canned fruit and vegetables, the topics that are reviewed and discussed are the effects of low pH on heat resistance of the most important microorganisms, acidification techniques and significant process variables, the effect of low pH on sensorial properties, and future trends.

  10. Using biological effects tools to define Good Environmental Status under the Marine Strategy Framework Directive

    NARCIS (Netherlands)

    Lyons, B.P.; Thain, J.E.; Hylland, K.; Davis, I.; Vethaak, A.D.

    2010-01-01

    The use of biological effects tools offer enormous potential to meet the challenges outlined by the European Union Marine Strategy Framework Directive (MSFD) whereby Member States are required to develop a robust set of tools for defining 11 qualitative descriptors of Good Environmental Status

  11. Forest fuel and sulphur

    International Nuclear Information System (INIS)

    Lundborg, A.

    1994-10-01

    This report illustrates the sulphur cycle in forest fuel and in the forest ecosystem. The hypothesis is that sulphur dioxide from combustion of forest fuel is not more acidifying than sulphur that is mineralized from tree biomass if it is left in the forest instead of being burnt. The report gives an overview of the sulphur cycle in general together with the acidifying effect of sulphur. The sulphur content in wood biomass is about 1 mg/g in the needles and 0.2-0.3 mg/g in wood. Chipped forest fuel contains 0.2-0.5 mg S/g. A removal of 40 tonnes of felling residues per hectare may contain about 8-30 kg S. The sulphur occurs both in organic, often reduced, form and as sulphate. In situations of high availability to sulphur there will be an increased proportion of sulphate. After combustion some, perhaps half, of the sulphur is left in the ashes, most of which appears to be sulphate. In mineralisation of reduced organic sulphur, of type R-SH, the sulphur is released in the form of sulphide. Hydrogen sulphide, H2S, can be oxidised by microbes to sulphate, which should be acidifying (2 H+ will remain). A very rough estimate suggests that emissions of sulphur dioxide from forest fuel, spread over the period the trees are growing, and on the area from which the trees are taken, corresponds to 0.5% of the sulphur deposition in southern Sweden. Sulphur emissions from biofuel combustion are much lower than Sweden's and the EU's most stringent emission limits for coal. Whole-tree removal with return of ashes will theoretically give a considerable reduction in soil acidity since large quantities of nitrogen are removed and thus the acidifying effect of nitrogen will not occur. This should be of greater importance for forest acidification than the effect of biomass sulphur. 80 refs, numerous tabs

  12. Effects of Extraction Solvents on the Quantification of Free Amino Acids in Lyophilised Brewer’s Yeast

    Directory of Open Access Journals (Sweden)

    Andreea STĂNILĂ

    2018-05-01

    Full Text Available The aim of this work was to test some solvents in order to improve the free amino acids extraction from lyophilised brewer’s yeast. The brewer’ yeast was treated with four types of extraction solvents: Solvent I – acetonitrile 25%/HCl 0.01M (ACN; Solvent II – ethanol 80%; solvent III – HCl 0.05M/deionized water (1/1 volume; Solvent IV – HCl 0.05M/ethanol 80% (1/1 volume. The supernatants were analysed by HPLC-DAD-ESI-MS method. Acetonitrile provided the less quantities and number of amino acids extracted due to its weaker polarity. Solvent II and IV (ethanol, respectively acidified ethanol, which have an increased polarity, extracted 15 amino acids due to the addition of HCl in solvent IV. Solvent III (acidified water proved to be the best extraction solvent for the amino acids from brewer’s yeast providing the separation of 17 compounds: GLN, ASN, SER, GLY, ALA, ORN, PRO, HIS, LYS, GLU, TRP, LEU, PHE, ILE, AAA, HPHE, TYR.

  13. The effect of long-term acidifying feeding on digesta organic acids, mineral balance, and bone mineralization in growing pigs

    DEFF Research Database (Denmark)

    Nørgaard, Jan Værum; Højberg, Ole; Sørensen, Kristina Ulrich

    2014-01-01

    Acidification of slurry through dietary manipulation of urinary pH is a means of mitigating nitrogen emission from pig production, but long-term effects of diet acidification on bone mineralization and mineral balance is less investigated. The objective was therefore to study the long-term effects...... of feeding benzoic acid (BA) and calcium chloride (CaCl2) on the mineral balance and microbial activity in the gastrointestinal tract of pigs. Four diets containing the combinations of 0 or 10 g/kg BA and 0 or 20 g/kg CaCl2 were fed to 24 pigs in a factorial design. For the diets without CaCl2, calcium...... carbonate (CaCO3) was added to provide equimolar levels of Ca. The pigs were fed the diets from 36 kg until slaughter at 113 kg BW, and they were housed in balance cages for 12 d from 60 to 66 kg BW. Supplementation of BA and/or CaCl2 had only minor effect on accumulation of digesta organic acids (acetate...

  14. Effective Trust-Aware E-learning Recommender System Based on Learning Styles and Knowledge Levels

    Science.gov (United States)

    Dwivedi, Pragya; Bharadwaj, Kamal K.

    2013-01-01

    In the age of information explosion, e-learning recommender systems (ELRSs) have emerged as the most essential tool to deliver personalized learning resources to learners. Due to enormous amount of information on the web, learner faces problem in searching right information. ELRSs deal with the problem of information overload effectively and…

  15. A voz do Brasil: Miguel Pereira e o discurso sobre o "imenso hospital" The voice of Brazil: Miguel Pereira and his speech on the "enormous hospital"

    Directory of Open Access Journals (Sweden)

    Dominichi Miranda de Sá

    2009-07-01

    Full Text Available Este trabalho reproduz e comenta importante fonte primária muito citada nos estudos em história da saúde no Brasil, mas pouco conhecida em sua integralidade. Trata-se do discurso do médico Miguel Pereira, proferido em outubro de 1916, e no qual se referiu à zona rural do país como 'um imenso hospital'. A interpretação consagrada dessa fonte é a de que sua repercussão teve persistente e decisiva influência em políticas públicas de saúde na primeira metade do século XX. Neste texto, aprofundo o conhecimento das circunstâncias históricas que geraram a elaboração dessa expressão-síntese dos 'males do Brasil', sobretudo no que se refere às discussões, então em curso, a respeito do recrutamento militar obrigatório e do Exército como 'escola de civismo'.The article reproduces and comments on an important primary source that is often cited in studies on Brazil's history of health but which few are familiar with in its entirety: the October 1916 speech by physician Miguel Pereira, in which he referred to rural Brazil as an "enormous hospital." The standard interpretation is that this source had a steady, decisive influence on public health policies in the first half of the twentieth century. In this text, I endeavor to deepen our knowledge of the historical circumstances which produced this expression that synthesized the 'evils of Brazil', especially in regard to the era's discussion of mandatory military recruitment and of the army as a 'civics school'.

  16. Legacy effects of continuous chloropicrin-fumigation for 3-years on soil microbial community composition and metabolic activity

    NARCIS (Netherlands)

    Zhang, Shuting; Liu, Xiaojiao; Jiang, Qipeng; Shen, Guihua; Ding, Wei

    2017-01-01

    Chloropicrin is widely used to control ginger wilt in China, which have an enormous impact on soil microbial diversity. However, little is known on the possible legacy effects on soil microbial community composition with continuous fumigation over different years. In this report, we used high

  17. Effects of Dietary Additives and Early Feeding on Performance, Gut Development and Immune Status of Broiler Chickens Challenged with

    Directory of Open Access Journals (Sweden)

    Z. Ao

    2012-04-01

    Full Text Available The effects of dietary additives and holding time on resistance and resilience of broiler chickens to Clostridium perfringens challenge were investigated by offering four dietary treatments. These were a negative control (basal, a positive control (Zn-bacitracin and two dietary additives, mannanoligosaccharides (MOS, and acidifier. Two holding times included (a immediate access to feed and water post hatch (FED and (b access to both feed and water 48 h post hatch (HELD. Chicks fed Zn-bacitracin had no intestinal lesions attributed to necrotic enteritis (NE, whereas chicks fed both MOS or acidifier showed signs of NE related lesions. All dietary treatments were effective in reducing the numbers of C. perfringens in the ileum post challenge. The FED chicks had heavier body weight and numerically lower mortality. The FED chicks also showed stronger immune responses to NE challenge, showing enhanced (p<0.05 proliferation of T-cells. Early feeding of the MOS supplemented diet increased (p<0.05 IL-6 production. The relative bursa weight of the FED chicks was heavier at d 21 (p<0.05. All the additives increased the relative spleen weight of the HELD chicks at d 14 (p<0.05. The FED chicks had increased villus height and reduced crypt depth, and hence an increased villus/crypt ratio, especially in the jejunum at d 14 (p<0.05. The same was true for the HELD chicks given dietary additives (p<0.05. It may be concluded that the chicks with early access to dietary additives showed enhanced immune response and gut development, under C. perfringens challenge. The findings of this study shed light on managerial and nutritional strategies that could be used to prevent NE in the broiler industry without the use of in-feed antibiotics.

  18. Effects of acidifying ocean conditions on growth and survival of two life stages of the blue crab, Callinectes sapidus.

    Science.gov (United States)

    Giltz, S.; Taylor, C.

    2016-02-01

    Blue crabs, Callinectes sapidus, begin their larval phase offshore and circulate for approximately 30 days before settling near shore. As crabs transition to the juvenile stage, they move into coastal or estuarine environments characterized by lower salinity. Presently the average pH of the ocean is 8.1, 30% down from the beginning of the industrial revolution and is forecasted to drop to 7.8 by 2100. Decreasing pH causes dissolution of calcium carbonate shells, but the overall effects on crustaceans, such as blue crabs, are unknown. This study investigated the effect of a lower pH environment on the growth, survival, carapace hardness and molt frequency of larval and juvenile blue crabs in the Northern Gulf of Mexico. Larval crabs showed delayed growth under low pH (7.8) conditions compared to crabs in a control (present day) pH (8.1) environment. Population crashes (complete mortality) were experienced in 55% of the low pH aquaria but not in any of the control aquaria, suggesting that acidification poses a mortality risk. Under low pH conditions the intermolt duration decreased in juveniles, but the body length and weight did not differ from crabs raised in the control pH. Larvae (in tanks that did not crash) and juveniles did not experience increased mortality from a lower pH, but there do appear to be sublethal effects on growth and molting that differ between life history stages.

  19. Groundwater acidification in the Senne region, Teutoburger Wald mountains -will the specification of limiting values protect drinking water?

    International Nuclear Information System (INIS)

    Luekewille, A.; Heuwinkel, B.

    1990-01-01

    The Senne region is a pleistocene glacial outwash landscape in northern Germany. It is particularly sensitive to entries of airborne pollution with acids and acidifying agents. The shallow groundwater is already acidified down to a depth of about 10 meters. This is proved by the low pH values ( [de

  20. Impact of ionic aluminium on extracellular phosphatases in acidified lakes

    Czech Academy of Sciences Publication Activity Database

    Bittl, T.; Vrba, Jaroslav; Nedoma, Jiří; Kopáček, Jiří

    2001-01-01

    Roč. 3, č. 9 (2001), s. 578-587 ISSN 1462-2912 R&D Projects: GA ČR GA206/97/0072; GA ČR GA206/00/0063 Keywords : acid phosphatases * pH effect * inhibition Subject RIV: DA - Hydrology ; Limnology Impact factor: 3.276, year: 2001

  1. Niclosamide is a proton carrier and targets acidic endosomes with broad antiviral effects.

    Science.gov (United States)

    Jurgeit, Andreas; McDowell, Robert; Moese, Stefan; Meldrum, Eric; Schwendener, Reto; Greber, Urs F

    2012-01-01

    Viruses use a limited set of host pathways for infection. These pathways represent bona fide antiviral targets with low likelihood of viral resistance. We identified the salicylanilide niclosamide as a broad range antiviral agent targeting acidified endosomes. Niclosamide is approved for human use against helminthic infections, and has anti-neoplastic and antiviral effects. Its mode of action is unknown. Here, we show that niclosamide, which is a weak lipophilic acid inhibited infection with pH-dependent human rhinoviruses (HRV) and influenza virus. Structure-activity studies showed that antiviral efficacy and endolysosomal pH neutralization co-tracked, and acidification of the extracellular medium bypassed the virus entry block. Niclosamide did not affect the vacuolar H(+)-ATPase, but neutralized coated vesicles or synthetic liposomes, indicating a proton carrier mode-of-action independent of any protein target. This report demonstrates that physico-chemical interference with host pathways has broad range antiviral effects, and provides a proof of concept for the development of host-directed antivirals.

  2. Niclosamide is a proton carrier and targets acidic endosomes with broad antiviral effects.

    Directory of Open Access Journals (Sweden)

    Andreas Jurgeit

    Full Text Available Viruses use a limited set of host pathways for infection. These pathways represent bona fide antiviral targets with low likelihood of viral resistance. We identified the salicylanilide niclosamide as a broad range antiviral agent targeting acidified endosomes. Niclosamide is approved for human use against helminthic infections, and has anti-neoplastic and antiviral effects. Its mode of action is unknown. Here, we show that niclosamide, which is a weak lipophilic acid inhibited infection with pH-dependent human rhinoviruses (HRV and influenza virus. Structure-activity studies showed that antiviral efficacy and endolysosomal pH neutralization co-tracked, and acidification of the extracellular medium bypassed the virus entry block. Niclosamide did not affect the vacuolar H(+-ATPase, but neutralized coated vesicles or synthetic liposomes, indicating a proton carrier mode-of-action independent of any protein target. This report demonstrates that physico-chemical interference with host pathways has broad range antiviral effects, and provides a proof of concept for the development of host-directed antivirals.

  3. Mechanical break junctions: enormous information in a nanoscale package.

    Science.gov (United States)

    Natelson, Douglas

    2012-04-24

    Mechanical break junctions, particularly those in which a metal tip is repeatedly moved in and out of contact with a metal film, have provided many insights into electronic conduction at the atomic and molecular scale, most often by averaging over many possible junction configurations. This averaging throws away a great deal of information, and Makk et al. in this issue of ACS Nano demonstrate that, with both simulated and real experimental data, more sophisticated two-dimensional analysis methods can reveal information otherwise obscured in simple histograms. As additional measured quantities come into play in break junction experiments, including thermopower, noise, and optical response, these more sophisticated analytic approaches are likely to become even more powerful. While break junctions are not directly practical for useful electronic devices, they are incredibly valuable tools for unraveling the electronic transport physics relevant for ultrascaled nanoelectronics.

  4. Effects of e-banking on growth of customer base in kenyan banks

    OpenAIRE

    Okibo, Bichanga Walter; Wario, Ali Yattani

    2014-01-01

    The study highlights the effects of E-banking services on growth of customer base in Kenyan banks for over the last five years. It addresses issues that affect effective utilization of E-banking facilities by customers. The study generally investigates how lack of technological know-how, illiteracy, unreliability, and transaction limits has hindered growth of on-line customer base in Kenyan banks. E-banking provides enormous benefits to consumers in terms of time saving and cost of transactio...

  5. Modulation of leak K(+) channel in hypoglossal motoneurons of rats by serotonin and/or variation of pH value.

    Science.gov (United States)

    Xu, Xue-Feng; Tsai, Hao-Jan; Li, Lin; Chen, Yi-Fan; Zhang, Cheng; Wang, Guang-Fa

    2009-08-25

    The cloned TWIK-related acid-sensitive K(+) channel (TASK-1) is sensitive to the pH changes within physiological pH range (pK~7.4). Recently, the native TASK-1-like channel was suggested to be the main contributor to the background (or leak) K(+) conductance in the motoneurons of the brain stem. Serotonin (5-HT) and variation of pH value in perfused solution could modulate these currents. Here we aimed to examine the properties and modulation of the currents by serotonin or variation of pH value in hypoglossal motoneurons of rats. Transverse slices were prepared from the brainstem of neonatal Sprague-Dawley rats (postnatal days 7-8). Hypoglossal motoneurons were used for the study. The leak K(+) current (TASK-1-like current) and hyperpolarization-activated cationic current (I(h)) were recorded with the whole-cell patch-clamp technique. The results showed that these currents were inhibited by acidified artificial cerebrospinal fluid (ACSF, pH 6.0) and activated by alkalized ACSF (pH 8.5). 5-HT (10 mumol/L) significantly inhibited both leak K(+) current and I(h) with depolarization of membrane potential and the occurrence of oscillation and/or spikes. Bath application of Ketanserine, an antagonist of 5-HT₂ receptor, reversed or reduced the inhibitory effect of acidified solution on leak K(+) current and I(h). The results suggest that 5-HT₂ receptors mediate the effects of acidified media on leak K(+) current and I(h) in hypoglossal motoneurons.

  6. Effect of co-solute and gelation temperature on milk protein and gum tragacanth interaction in acidified gels.

    Science.gov (United States)

    Hatami, Masoud; Nejatian, Mohammad; Mohammadifar, Mohammad Amin

    2012-05-01

    The aim of this study was to investigate the role of process conditions and system composition on the acid-induced gelation of a mixture of milk protein and gum tragacanth. This was studied by determining the effects of co-solute (lactose) addition (3, 5 and 7%) and gelation temperature (25, 37 and 45°C) on the mixture's rheological properties and microstructure using a combination of techniques including small-deformation rheology and scanning electron microscopy. The presence of lactose played an important role in the microstructure formation of gels but did not change most rheological properties. The microstructure of gels formed in the presence of lactose was coarser and more particulate, but less interconnected; this can be explained by lactose's role in improving protein aggregation. Gels prepared at a lower temperature had a high structure strength, as indicated by their high storage modulus, τ(f) and G(f) values. Low gelation temperature also caused a more branched and homogenous microstructure. Copyright © 2012 Elsevier B.V. All rights reserved.

  7. Microbial bio-based plastics from olive-mill wastewater: Generation and properties of polyhydroxyalkanoates from mixed cultures in a two-stage pilot scale system.

    Science.gov (United States)

    Ntaikou, I; Valencia Peroni, C; Kourmentza, C; Ilieva, V I; Morelli, A; Chiellini, E; Lyberatos, G

    2014-10-20

    The operational efficiency of a two stage pilot scale system for polyhydroxyalkanoates (PHAs) production from three phase olive oil mill wastewater (OMW) was investigated in this study. A mixed anaerobic, acidogenic culture derived from a municipal wastewater treatment plant, was used in the first stage, aiming to the acidification of OMW. The effluent of the first bioreactor that was operated in continuous mode, was collected in a sedimentation tank in which partial removal of the suspended solids was taking place, and was then forwarded to an aerobic reactor, operated in sequential batch mode under nutrient limitation. In the second stage an enriched culture of Pseudomonas sp. was used as initial inoculum for the production of PHAs from the acidified waste. Clarification of the acidified waste, using aluminium sulphate which causes flocculation and precipitation of solids, was also performed, and its effect on the composition of the acidified waste as well as on the yields and properties of PHAs was investigated. It was shown that clarification had no significant qualitative or quantitative effect on the primary carbon sources, i.e. short chain fatty acids and residual sugars, but only on the values of total suspended solids and total chemical oxygen demand of the acidified waste. The type and thermal characteristics of the produced PHAs were also similar for both types of feed. However the clarification of the waste seemed to have a positive impact on final PHAs yield, measured as gPHAs/100g of VSS, which reached up to 25%. Analysis of the final products via nuclear magnetic resonance spectroscopy revealed the existence of 3-hydroxybutyrate (3HB) and 3-hydroxyoctanoate (HO) units, leading to the conclusion that the polymer could be either a blend of P3HB and P3HO homopolymers or/and the 3HB-co-3HO co-polymer, an unusual polymer occurring in nature with advanced properties. Copyright © 2014 Elsevier B.V. All rights reserved.

  8. Chemoreception of the Seagrass Posidonia Oceanica by Benthic Invertebrates is Altered by Seawater Acidification.

    Science.gov (United States)

    Zupo, Valerio; Maibam, Chingoileima; Buia, Maria Cristina; Gambi, Maria Cristina; Patti, Francesco Paolo; Scipione, Maria Beatrice; Lorenti, Maurizio; Fink, Patrick

    2015-08-01

    Several plants and invertebrates interact and communicate by means of volatile organic compounds (VOCs). These compounds may play the role of infochemicals, being able to carry complex information to selected species, thus mediating inter- or intra-specific communications. Volatile organic compounds derived from the wounding of marine diatoms, for example, carry information for several benthic and planktonic invertebrates. Although the ecological importance of VOCs has been demonstrated, both in terrestrial plants and in marine microalgae, their role as infochemicals has not been demonstrated in seagrasses. In addition, benthic communities, even the most complex and resilient, as those associated to seagrass meadows, are affected by ocean acidification at various levels. Therefore, the acidification of oceans could produce interference in the way seagrass-associated invertebrates recognize and choose their specific environments. We simulated the wounding of Posidonia oceanica leaves collected at two sites (a control site at normal pH, and a naturally acidified site) off the Island of Ischia (Gulf of Naples, Italy). We extracted the VOCs and tested a set of 13 species of associated invertebrates for their specific chemotactic responses in order to determine if: a) seagrasses produce VOCs playing the role of infochemicals, and b) their effects can be altered by seawater pH. Our results indicate that several invertebrates recognize the odor of wounded P. oceanica leaves, especially those strictly associated to the leaf stratum of the seagrass. Their chemotactic reactions may be modulated by the seawater pH, thus impairing the chemical communications in seagrass-associated communities in acidified conditions. In fact, 54% of the tested species exhibited a changed behavioral response in acidified waters (pH 7.7). Furthermore, the differences observed in the abundance of invertebrates, in natural vs. acidified field conditions, are in agreement with these behavioral

  9. Effects of acid deposition on microbial processes in natural waters

    International Nuclear Information System (INIS)

    Gilmour, C.C.

    1992-01-01

    Biogeochemical processes mediated by microorganisms are not adversely affected by the acidification of natural waters to the same extent as are the life cycles of higher organisms. Basic processes, e.g., primary production and organic matter decomposition, are not slowed in moderately acidified systems and do not generally decline above a pH of 5. More specifically, the individual components of the carbon, nitrogen, and sulfur cycles are, with few exceptions, also acid resistant. The influence of acid deposition on microbial processes is more often stimulation of nitrogen and sulfur cycling, often leading to alkalinity production, which mitigates the effect of strong acid deposition. Bacterial sulfate reduction and denitrification in sediments are two of the major processes that can be stimulated by sulfate and nitrate deposition, respectively, and result in ANC (acid-neutralizing capacity) generation. One of the negative effects of acid deposition is increased mobilization and bioaccumulation of some metals. Bacteria appear to play an important role, especially in mercury cycling, with acidification leading to increased bacterial methylation of mercury and subsequent bioaccumulation in higher organisms

  10. Modulating the aggregation behaviour to restore the mechanical response of acid induced mixed gels of sodium caseinate and soy proteins

    NARCIS (Netherlands)

    Martin, Anneke H.; Los Reyes Jiménez, De Marta L.; Pouvreau, Laurice

    2016-01-01

    Partial replacement of milk proteins with plant proteins is a challenge due to the reported negative effect on physical and sensory properties. Understanding of how the mechanical properties of acidified milk gels can be restored when 30% casein is replaced with soy proteins is therefore

  11. Modulating the aggregation behaviour to restore the mechanical response of acid induced mixed gels of sodium caseinate and soy proteins

    NARCIS (Netherlands)

    Martin, A.H.; Reyes Jiménez, M. L. de los; Pouvreau, L.

    2016-01-01

    Partial replacement of milk proteins with plant proteins is a challenge due to the reported negative effect on physical and sensory properties. Understanding of how the mechanical properties of acidified milk gels can be restored when 30% casein is replaced with soy proteins is therefore explored.

  12. Recovery of anthocyanins from residues of Rubus fruticosus, Vaccinium myrtillus and Eugenia brasiliensis by ultrasound assisted extraction, pressurized liquid extraction and their combination.

    Science.gov (United States)

    Machado, Ana Paula Da Fonseca; Pereira, Ana Luiza Duarte; Barbero, Gerardo Fernández; Martínez, Julian

    2017-09-15

    This work investigated the extraction efficiency of polyphenols (anthocyanins) from blackberry, blueberry and grumixama residues using combined ultrasonic assisted extraction (UAE) and pressurized liquid extraction (PLE) (UAE+PLE). The performance of UAE+PLE was compared to those achieved by the isolated PLE and UAE methods and conventional Soxhlet extraction. The effects of the extraction methods and solvents (acidified water pH 2.0, ethanol+water 50% v/v and ethanol+water 70% ethanol v/v) on total phenolics content, anthocyanin composition and antioxidant capacity of extracts were investigated by a full factorial design. The extraction efficiency for total phenolics and antioxidant capacity in decreasing order was: UAE+PLE>PLE≈Soxhlet>UAE, and for anthocyanins it was: Soxhlet≈UAE>UAE+PLE>PLE, using hydroethanolic mixtures as solvents. Extractions with acidified water and ultrasound were not effective to recover phenolics. Two, four and fourteen anthocyanins were identified in the extracts from grumixama, blackberry and blueberry, respectively. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Technological properties of Lactobacillus fermentum involved in the processing of dolo and pito, West African sorghum beers, for the selection of starter cultures

    DEFF Research Database (Denmark)

    Sawadogo-Lingani, H.; Diawara, B.; Traoré, A.S.

    2008-01-01

    AIM: Technological properties of Lactobacillus fermentum isolates involved in spontaneous fermentation of dolo and pito wort were examined to select starter cultures. METHODS AND RESULTS: 264 isolates were screened for antimicrobial activity, acidifying activity, exopolysaccharides (EPSs) and amy......AIM: Technological properties of Lactobacillus fermentum isolates involved in spontaneous fermentation of dolo and pito wort were examined to select starter cultures. METHODS AND RESULTS: 264 isolates were screened for antimicrobial activity, acidifying activity, exopolysaccharides (EPSs...... the isolates which were clustered in four groups according to their rates of acidification of sorghum malt broth. The faster acidifying group of isolates (43.48%) had a rate of acidification evaluated as Delta pH of 1.14 +/- 0.15 pH unit after 6 h of fermentation, followed by a second group of isolates (38.......08%) with a similar rate of acidification after 9 h of fermentation. From the isolates endowed with an antimicrobial activity, 5.76% belonged to the faster acidifying group and 40.38% belonged to the second group. 88.7% of the isolates had the ability for producing EPSs but not amylase. CONCLUSION: Lactobacillus...

  14. The Effects of Lime, Fertilizer, and Herbicide on Forest Soil Solution Chemistry and Northern Red Oak Radial Growth Following Shelterwood Harvest

    Science.gov (United States)

    Angela M Happel; William E. Sharpe

    2004-01-01

    Soil acidity, nutrient deficient soils, lack of light penetration, herbivory, and understory competition are the major obstacles encountered in regenerating and sustaining northern red oak. Changes in soils that may occur during soil acidifi- cation include: reduced soil pH, increased availability of aluminum (Al) and manganese (Mn), loss of base cations due to...

  15. Effects of acidified seawater on coral calcification and variations of U/Ca ratio in their skeletons

    Science.gov (United States)

    Inoue, M.; Ozaki, S.; Iguchi, A.; Sakai, K.; Suzuki, A.; Kawahata, H.

    2011-12-01

    The rising CO2 concentration in the atmosphere is changing the carbonate chemistry of the ocean. Elevated partial pressure of CO2 (pCO2) has caused significant decrease in surface seawater pH and carbonate ion concentration. Therefore, ocean acidification has a negative effect on calcification of marine calcifying organisms. Especially, hermatypic corals are dominant organisms in coral reef ecosystems, so their calcificication is a key to determine the health of reef ecosystems. On the other hand, recent study has suggested that there is a negative correlation between U/Ca ratio in coral skeleton and seawater pH, based on the culture experiment using primary polyps of Acropora digitifera. In this study, primary polyps and adult colonies of A. digitifera and adult colonies of Porites australiensis, which are the dominant species around the Ryukyu Islands, Japan, were reared in seawater with different pCO2 (300, 400, 600, 800, 1000ppm) and pH (7.4, 7.6, 8.0) settings controlled by CO2 bubbling. Calcification rate of adult coral was estimated by buoyant method, while skeletal growth of polyps was evaluated by measuring the dry weight of each skeleton after the experiments. In order to evaluate the relationship between U/Ca ratios in coral skeletons and seawater pH, U/Ca ratios in reared corals were analyzed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The results of A. digitifera showed that the growth rate of adult corals had no significant correlation against pCO2, but dry weight of polyp skeletons decreased with increase in pCO2. Growth rate of P. australiensis typically showed a positive correlation with pH. However, growth rates were different among colonies, suggesting that their responses to acidification may vary among the colonies. Regarding the variations of U/Ca ratios, there were positive correlations between U/Ca ratios in adults of A. digitifera and P. australiensis and seawater pCO2 (pH), while no relation was observed in polyp corals.

  16. Optimisation and inhibition of anaerobic digestion of livestock manure

    Energy Technology Data Exchange (ETDEWEB)

    Sutaryo, S.

    2012-11-15

    The optimisation process during this PhD study focused on mixed enzyme (ME) addition, thermal pre-treatment and co-digestion of raw manure with solid fractions of acidified manure, while for inhibition processes, ammonia and sulphide inhibition were studied. ME addition increased methane yield of both dairy cow manure (DCM) and solid fractions of DCM (by 4.44% and 4.15% respectively, compared to the control) when ME was added to manure and incubated prior to anaerobic digestion (AD). However, no positive effect was found when ME was added to manure and fed immediately to either mesophilic (35 deg. C) or thermophilic (50 deg. C) digesters. Low-temperature pre-treatment (65 deg. C to 80 deg. C for 20 h) followed by batch assays increased the methane yield of pig manure in the range from 9.5% to 26.4% at 11 d incubation. These treatments also increased the methane yield of solid-fractions pig manure in the range from 6.1% to 25.3% at 11 d of the digestion test. However, at 90 d the increase in methane yield of pig manure was only significant at the 65 deg. C treatment, thus low-temperature thermal pre-treatment increased the rate of gas production, but did not increase the ultimate yield (B{sub o}). High-temperature pre-treatment (100 deg. C to 225 deg. C for 15 min.) increased the methane yield of DCM by 13% and 21% for treatments at 175 deg. C and 200 deg. C, respectively, at 27 d of batch assays. For pig manure, methane yield was increased by 29% following 200 deg. C treatment and 27 d of a batch digestion test. No positive effect was found of high-temperature pre-treatment on the methane yield of chicken manure. At the end of the experiment (90 d), high-temperature thermal pre-treatment was significantly increasing the B{sub 0} of pig manure and DCM. Acidification of animal manure using sulphuric acid is a well-known technology to reduce ammonia emission of animal manure. AD of acidified manure showed sulphide inhibition and consequently methane production was 45

  17. Predicting nitrogen and acidity effects on long-term dynamics of dissolved organic matter

    OpenAIRE

    Rowe, E.C.; Tipping, E.; Posch, M.; Oulehle, Filip; Cooper, D.M.; Jones, T.G.; Burden, A.; Hall, J.; Evans, C.D.

    2014-01-01

    Increases in dissolved organic carbon (DOC) fluxes may relate to changes in sulphur and nitrogen pollution. We integrated existing models of vegetation growth and soil organic matter turnover, acid-base dynamics, and organic matter mobility, to form the ‘MADOC’ model. After calibrating parameters governing interactions between pH and DOC dissolution using control treatments on two field experiments, MADOC reproduced responses of pH and DOC to additions of acidifying and alkalising solutions. ...

  18. Transgenerational deleterious effects of ocean acidification on the reproductive success of a keystone crustacean (Gammarus locusta).

    Science.gov (United States)

    Borges, Francisco O; Figueiredo, Cátia; Sampaio, Eduardo; Rosa, Rui; Grilo, Tiago F

    2018-07-01

    Ocean acidification (OA) poses a global threat to marine biodiversity. Notwithstanding, marine organisms may maintain their performance under future OA conditions, either through acclimation or evolutionary adaptation. Surprisingly, the transgenerational effects of high CO 2 exposure in crustaceans are still poorly understood. For the first time, the present study investigated the transgenerational effect of OA, from hatching to maturity, of a key amphipod species (Gammarus locusta). Negative transgenerational effects were observed on survival of the acidified lineage, resulting in significant declines (10-15%) compared to the control groups in each generation. Mate-guarding duration was also significantly reduced under high CO 2 and this effect was not alleviated by transgenerational acclimation, indicating that precopulatory behaviours can be disturbed under a future high CO 2 scenario. Although OA may initially stimulate female investment, transgenerational exposure led to a general decline in egg number and fecundity. Overall, the present findings suggest a potential fitness reduction of natural populations of G. locusta in a future high CO 2 ocean, emphasizing the need of management tools towards species' sustainability. Copyright © 2018 Elsevier Ltd. All rights reserved.

  19. Performance and carcass characteristics of Japanese quail as ...

    African Journals Online (AJOL)

    The effect of sex and the supplementation of the prebiotic, mannan oligosaccharides (MOS), the acidifier, calcium propionate (CPr) or their combination in the feed of Japanese quail (Coturnix japonica) on their performance and carcass quality was examined in this experimentation. Three hundred, 1-day old Japanese quail ...

  20. Elevated ammonium levels

    DEFF Research Database (Denmark)

    Søgaard, Rikke; Novak, Ivana; MacAulay, Nanna

    2012-01-01

    was not enhanced. The ammonium-induced stimulation of EAAT3 increased with increasing extracellular pH, suggesting that the gaseous form NH(3) mediates the effect. An ammonium-induced intracellular alkalinization was excluded as the cause of the enhanced EAAT3 activity because 1) ammonium acidified the oocyte...

  1. Base Cation Leaching From the Canopy of a Rubber ( Hevea ...

    African Journals Online (AJOL)

    Base cations are essential to the sustainability of forest ecosystems. They are important for neutralizing the acidifying effects of atmospheric deposition. There is the need for in-depth understanding of base cation depletion and leaching from forest canopy. This is important particularly due to the increasing acidification and ...

  2. Evaluation through column leaching tests of metal release from contaminated estuarine sediment subject to CO2 leakages from Carbon Capture and Storage sites

    International Nuclear Information System (INIS)

    Payán, M. Cruz; Galan, Berta; Coz, Alberto; Vandecasteele, Carlo; Viguri, Javier R.

    2012-01-01

    The pH change and the release of organic matter and metals from sediment, due to the potential CO 2 acidified seawater leakages from a CCS (Carbon Capture and Storage) site are presented. Column leaching test is used to simulate a scenario where a flow of acidified seawater is in contact with recent contaminated sediment. The behavior of pH, dissolved organic carbon (DOC) and metals As, Cd, Cr, Cu, Ni, Pb, Zn, with liquid to solid (L/S) ratio and pH is analyzed. A stepwise strategy using empirical expressions and a geochemical model was conducted to fit experimental release concentrations. Despite the neutralization capacity of the seawater-carbonate rich sediment system, important acidification and releases are expected at local scale at lower pH. The obtained results would be relevant as a line of evidence input of CCS risk assessment, in an International context where strategies to mitigate the climate change would be applied. - Highlights: ► Tier structured approach for assessment of the release of metals from sediment. ► Standard column leaching test to simulate CO 2 acidified seawater CCS leakages. ► Metal and DOC release from marine sediment in contact to CO 2 acidified seawater. ► From empirical to geochemical modeling approaches of DOC and metals release in column tests. ► Contamination line of evidence input of CCS risk assessment. - Column metal release from CO 2 acidified seawater leakages in contact with estuarine contaminated sediment in CCS sites

  3. Efficacy of MBA: On the Role of Network Effects in Influencing the Selection of Elective Courses

    Science.gov (United States)

    Roy, Vivek; Parsad, Chandan

    2018-01-01

    Purpose: The purpose of this paper is to outline the importance of social network effects in influencing the elective (courses) selection among masters of business administration (MBA) students and its role in influencing the efficacy of MBA. As such, given the enormous time and investment required for students to pursue an MBA and the role of…

  4. Pseudospins and Topological Effects of Phonons in a Kekulé Lattice

    Science.gov (United States)

    Liu, Yizhou; Lian, Chao-Sheng; Li, Yang; Xu, Yong; Duan, Wenhui

    2017-12-01

    The search for exotic topological effects of phonons has attracted enormous interest for both fundamental science and practical applications. By studying phonons in a Kekulé lattice, we find a new type of pseudospin characterized by quantized Berry phases and pseudoangular momenta, which introduces various novel topological effects, including topologically protected pseudospin-polarized interface states and a phonon pseudospin Hall effect. We further demonstrate a pseudospin-contrasting optical selection rule and a pseudospin Zeeman effect, giving a complete generation-manipulation-detection paradigm of the phonon pseudospin. The pseudospin and topology-related physics revealed for phonons is general and applicable for electrons, photons, and other particles.

  5. Growth and nutrition of coniferous forests on acidic mineral soils - status and effects of liming and fertilization

    International Nuclear Information System (INIS)

    Sikstroem, Ulf

    2001-01-01

    Deposited air-borne S- and N- containing pollutants acidify forest soils in southern Sweden. It has been suggested that this may severely affect forest yield. Liming and/or application of specific nutrients, e.g. phosphorus (P) and potassium (K), have been proposed as countermeasures. The influence of such measures, and of nitrogen (N) addition, was investigated in two experimental series over 5-10 years. Stem growth and needle element concentrations were assessed, predominantly in high-yielding Norway spruce [Picea abies (L.) Karst.] and Scots pine (Pinus sylvestris L.) stands, 30-60 years old, growing on acidic mineral soils in southern Sweden. The effect on crown transparency was also evaluated in some of the Norway spruce stands. The treatments included liming (500-6,000 kg ha -1 ), and N addition at low annual doses (2x10 kg N ha -1 ) and in single shots (150 kg N ha -1 ). Combinations of lime+N, lime+PK and lime+PKN were also tested. The effects were generally weak or negligible, except that growth was significantly increased by N fertilization in the Scots pine stands, and by lime+PKN in some of the Norway spruce stands. In another study, the survival and growth of Norway spruce seedlings were found to be more or less unaffected when planted in pre-harvest acidified, limed or N fertilized soil, although the Ca and Zn concentrations in their needles rose after liming, while those of Mn and Al declined. In closed-canopy stands of Norway spruce and Scots pine with N concentrations of more than 15-16 mg (g DM) -1 in current-year needles, N fertilization was indicated to not necessarily stimulate increased growth. Other indicators of highly N-rich forests (e.g. elevated arginine levels) also start to appear above this level. The closed-canopy stands growing on the most acidic soils showed no signs of severe damage or nutrient deficiencies. These findings, together with the small or negligible effects of the tested countermeasures against soil acidification

  6. Effects of 12 weeks of treatment with fermented milk on blood pressure, glucose metabolism and markers of cardiovascular risk in patients with type 2 diabetes

    DEFF Research Database (Denmark)

    Hove, K D; Brøns, C; Færch, K

    2015-01-01

    helveticus on BP, glycaemic control and cardiovascular risk factors in T2D. DESIGN: A randomised, double-blinded, prospective, placebo-controlled study. METHODS: In one arm of a factorial study design, 41 patients with T2D were randomised to receive 300 ml milk fermented with L. helveticus (Cardi04 yogurt......OBJECTIVE: Studies have indicated a blood pressure (BP)-lowering effect of milk-derived peptides in non-diabetic individuals, but the cardiometabolic effects of such peptides in patients with type 2 diabetes (T2D) are not known. We investigated the effect of milk fermented with Lactobacillus......) (n=23) or 300 ml artificially acidified milk (placebo yogurt) (n=18) for 12 weeks. BPs were measured over 24-h, and blood samples were collected in the fasting state and during a meal test before and after the intervention. RESULTS: Cardi04 yogurt did not reduce 24-h, daytime or nighttime systolic...

  7. Hematological parameters on the effect of the jellyfish venom Cassiopea andromeda in animal models

    Directory of Open Access Journals (Sweden)

    Iraj Nabipour

    2017-04-01

    Full Text Available For the first time, we previously recorded an enormous population of the Cassiopea andromeda jellyfish that had increased dramatically from Bushehr coasts of Iran. The sub-acute toxicity of the jellyfish venom in rat organs was correspondingly carried out. The data presented in this paper relate to the in vivo and in vitro hematological effects of this venomous species of jellyfish venom.

  8. An investigation of the health hazards of some of the chemical ...

    African Journals Online (AJOL)

    This short piece takes a limited look at the health hazards of the chemical contents of seven fruit juices sold in The Gambia. All of them have very negative effects on health. The following additives were considered: sweeteners, coloring agents, flavoring agents and acidifiers vis-à-vis established studies. [African Journal of ...

  9. Inhibitory effects of crude extracts from several plants on postharvest pathogens of citrus

    Science.gov (United States)

    Gong, Mingfu; Guan, Qinlan; Xu, Shanshan

    2018-04-01

    China is one of the most important origin of citrus. Enormous economic losses was caused by fungal diseases in citrus harvest storage every year. The effective antimicrobial substances of garlic, ginger, celery and pepper were extracted by ethanol extraction and water extraction respectively. The inhibitory effects of the crude extract on Penicillium sp. caused fungal diseases in citrus harvest storage were also determined. The results showed that the extracts of garlic, ginger and celery had inhibitory effect on P. sp., but the extracts of pepper had no inhibitory effect on P. sp.. The garlic ethanol extracts had the best inhibitory effect on P. citrinum.

  10. Extraction of cesium and strontium from nuclear waste

    Science.gov (United States)

    Davis, Jr., Milton W.; Bowers, Jr., Charles B.

    1988-01-01

    Cesium is extracted from acidified nuclear waste by contacting the waste with a bis 4,4'(5) [1-hydroxy-2-ethylhexyl]benzo 18-crown-6 compound and a cation exchanger in a matrix solution. Strontium is extracted from acidified nuclear waste by contacting the waste with a bis 4,4'(5') [1-hydroxyheptyl]cyclohexo 18-crown-6 compound, and a cation exchanger in a matrix solution.

  11. Clean-up and matrix effect in LC-MS/MS analysis of food of plant origin for high polar herbicides.

    Science.gov (United States)

    Kaczyński, Piotr

    2017-09-01

    This study reports an innovative and sensitive procedure for analysis of difficult high polar herbicides (HPH) in diverse foods of plant origin. The QuPPe (Quick Polar Pesticides) method followed by determination by LC-MS/MS was modified. Chromatographic conditions, extraction, clean-up, and matrix effect were studied. Several liquid chromatography stationary and mobile phases were evaluated, and it was found that hydrophilic interaction chromatography (HILIC) gives good retention and sensitivity. An acidified methanol-water mixture was used as an effective extraction solvent of eleven HPH. Dispersive solid-phase clean-up sorbents (C18, GCB, Florisil, chitosan and graphene) were evaluated. The efficiency of the method was examined using data on recovery, precision and matrix effects. High extraction yields were achieved, and recoveries were within the 64-97% range with relative standard deviations <20% for all HPH in all commodities. Low matrix effects were observed when graphene was used during clean-up of onion extract and when chitosan was used for wheat, potato and pea extract. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Long term effects of low doses of ionising radiation: facts and fallacies

    International Nuclear Information System (INIS)

    Iyer, G.K.

    1993-01-01

    Health effects of low doses of ionising radiation have been a public concern. The public perception of these low effects is that it causes cancer and genetic effects. Enormous amount of work regarding this cancer has been done all over the world, on occupational workers exposed to low doses of ionising radiation. These studies do not show any adverse effect on them. Epidemiological studies done on members of public staying near nuclear facilities also have shown that there is no health risk involved in staying near these facilities. Genetic effects have also shown negative results. These two aspects of health effects of low dose of radiation are discussed in detail. (author). 5 refs., 1 tab

  13. The effects of different fertilizers on the ciliate communities of forests

    International Nuclear Information System (INIS)

    Lehle, E.; Funke, W.

    1993-01-01

    The influence of lime, mineral fertilizers and different composts on the ciliate communities (Protozoa: Ciliophora) of very acidified spruce forests were tested. In succession culture experiments species range, total numbers, abundance and dominance structure of the ciliate communities showed continued changes over a 90 day period. This method offers the opportunity not only to diagnose, but also to quantify effects of soil treatments. Investigations at the ARINUS-site Schluchsee (Black Forest) showed the following results: Liming led to a greater change in the ciliate communities than fertilization with magnesium-sulphate and ammonia-sulphate. Liming and treatment with compost, made from household garbage, and compost, made from chopped wood on the Beimerstetten site (Swabian Alb) led also to remarkably changes in the ciliate communities. The total ciliate numbers of the household garbage area were very low. In contrast, the ciliate community of the chopped wood compost areas were characterized by a great species number and high abundances of spirotrichuous ciliates, especially Halteria grandinella. (orig./UWA) [de

  14. Elaboration of the model for the assessment of outdoor advertising effectiveness

    OpenAIRE

    Pilelienė, Lina; Grigaliūnaitė, Viktorija

    2016-01-01

    In today's market economy organizations compete for consumers' attention, because in such information overload there is a high possibility of the advertisement to be not noticed, in which case it has zero probability to become effective. Outdoor advertising presents a unique case in that, unlike advertising in television or magazines, an amount of factors that distract (or can attract consumers' attention is enormous. The scientific problem solved by this research is defined by a question: wh...

  15. Altered epiphyte community and sea urchin diet in Posidonia oceanica meadows in the vicinity of volcanic CO2 vents.

    Science.gov (United States)

    Nogueira, Patricia; Gambi, Maria Cristina; Vizzini, Salvatrice; Califano, Gianmaria; Tavares, Ana Mafalda; Santos, Rui; Martínez-Crego, Begoña

    2017-06-01

    Ocean acidification (OA) predicted for 2100 is expected to shift seagrass epiphyte communities towards the dominance of more tolerant non-calcifying taxa. However, little is known about the indirect effects of such changes on food provision to key seagrass consumers. We found that epiphyte communities of the seagrass Posidonia oceanica in two naturally acidified sites (i.e. north and south sides of a volcanic CO 2 vent) and in a control site away from the vent at the Ischia Island (NW Mediterranean Sea) significantly differed in composition and abundance. Such differences involved a higher abundance of non-calcareous crustose brown algae and a decline of calcifying polychaetes in both acidified sites. A lower epiphytic abundance of crustose coralline algae occurred only in the south side of the vents, thus suggesting that OA may alter epiphyte assemblages in different ways due to interaction with local factors such as differential fish herbivory or hydrodynamics. The OA effects on food items (seagrass, epiphytes, and algae) indirectly propagated into food provision to the sea urchin Paracentrotus lividus, as reflected by a reduced P. oceanica exploitation (i.e. less seagrass and calcareous epiphytes in the diet) in favour of non-calcareous green algae in both vent sites. In contrast, we detected no difference close and outside the vents neither in the composition of sea urchin diet nor in the total abundance of calcareous versus non-calcareous taxa. More research, under realistic scenarios of predicted pH reduction (i.e. ≤ 0.32 units of pH by 2100), is still necessary to better understand cascading effects of this altered urchin exploitation of food resources under acidified conditions on ecosystem diversity and function. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Elusive prize: enormous coal gas potential awaits production technology breakthrough

    Energy Technology Data Exchange (ETDEWEB)

    Collison, M.

    2002-01-07

    The expanded gas pipeline grid has excess capacity, and gas resources are declining. There is increasing interest in development of Canada's resources of coalbed methane (CBM). The chairman of the Canadian Coalbed Methane Forum estimates that Canada has more than 3,000 trillion ft{sup 3} of gas awaiting suitable technology. PanCanadian and MGV Energy conducted a CBM exploration and pilot study on the Palliser spread in southern Alberta. Results from 23 of 75 wells are encouraging. The study is being accelerated and expanded to include an additional 50 wells elsewhere in Alberta. Some scientists anticipate commercial CBM production within two years. Problems facing developers include the large land holdings necessary for economic CBM production and the disposal of coal formation water. It is anticipated that U.S. technology will be modified and used. The potential for CBM development at Pictou in Nova Scotia and in British Columbia in the foothills is considered. 3 figs.

  17. Atypical presentation of an enormous vesical calculus: A case report

    African Journals Online (AJOL)

    A.T. Lawal

    2016-05-20

    May 20, 2016 ... Abstract. Giant vesical calculi, though rare, are still encountered in contemporary urologic practice despite the increased availability, accessibility, and affordability of modern imaging modalities and urologic care. This is the case report of a middle-aged man with an unusually large vesical calculus.

  18. 21 CFR 131.111 - Acidified milk.

    Science.gov (United States)

    2010-04-01

    ... such ingredients. (2) Nutritive carbohydrate sweeteners. Sugar (sucrose), beet or cane; invert sugar (in paste or sirup form); brown sugar; refiner's sirup; molasses (other than blackstrap); high..., dried malt extract; malt sirup, dried malt sirup; honey; maple sugar; or any of the sweeteners listed in...

  19. Recovery of acidified European surface waters

    Czech Academy of Sciences Publication Activity Database

    Wright, R. F.; Larssen, T.; Camarero, L.; Cosby, B. J.; Ferrier, R. C.; Helliwell, R.; Forsius, M.; Jenkins, A.; Kopáček, Jiří; Majer, V.; Moldan, F.; Posch, M.; Rogora, M.; Schöpp, W.

    2005-01-01

    Roč. 39, č. 3 (2005), 64A-72A ISSN 0013-936X. [ Acid Rain 2005. International Conference on Acid Deposition /7./. Prague, 12.06.2005-17.06.2005] Grant - others:EC(XE) EMERGE EVK1-CT-1999-00032; EC(XE) RECOVER:2010 EVK1-CT-1999-00018; DEFRA(GB) EPG 1/3/194; ICST(ES) REN2000-0889/GLO Institutional research plan: CEZ:AV0Z60170517 Keywords : acid ification * recovery * European lake districts Subject RIV: DJ - Water Pollution ; Quality Impact factor: 4.054, year: 2005

  20. Dual field effects in electrolyte-gated spinel ferrite: electrostatic carrier doping and redox reactions

    OpenAIRE

    Takashi Ichimura; Kohei Fujiwara; Hidekazu Tanaka

    2014-01-01

    Controlling the electronic properties of functional oxide materials via external electric fields has attracted increasing attention as a key technology for next-generation electronics. For transition-metal oxides with metallic carrier densities, the electric-field effect with ionic liquid electrolytes has been widely used because of the enormous carrier doping capabilities. The gate-induced redox reactions revealed by recent investigations have, however, highlighted the complex nature of the ...

  1. Effects of pH on embryo tolerance and adult behavior in the tiger salamander, Ambystoma tigrinum tigrinum

    Energy Technology Data Exchange (ETDEWEB)

    Whiteman, H H; Howard, R D; Whitten, K A [Purdue Univ., Lafayette, IN (United States). Dept. of Biological Sciences

    1995-08-01

    Adult discrimination ability and embryo performance was examined under different pH conditions in the eastern tiger salamander. Individuals from three populations were collected in habitats that differed naturally in pH. Two pH treatments were used to determine adult pH discrimination ability, and eight pH treatments to evaluate embryo performance. Results suggested that the pH of the source-population habitat could influence breeding-habitat discrimination by adults. Decreasing pH produced similar patterns of lethal and sublethal effects on embryos from the three populations, with reduced performance at low pH. The pH at which 50% mortality occurs was estimated at 4.2, suggesting that tiger salamanders were relatively acid tolerant. The study suggested that adult behavior patterns could influence the success of population reintroductions to previously acidified areas. 78 refs., 2 tabs., 4 figs.

  2. Economics of feeding drinking water containing organic acids to ...

    African Journals Online (AJOL)

    A feeding trial was conducted to determine the economic effect of acidifying drinking water of broiler chickens with organic acids. The organic acids were acetic, butyric, citric and formic acids, each offered at 0.25%. The control did not contain any of the acids. One hundred and fifty (150) day old AborAcre - plus chicks were ...

  3. V-ATPase as an effective therapeutic target for sarcomas

    Energy Technology Data Exchange (ETDEWEB)

    Perut, Francesca, E-mail: francesca.perut@ior.it [Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, Istituto Ortopedico Rizzoli, Bologna (Italy); Avnet, Sofia; Fotia, Caterina; Baglìo, Serena Rubina; Salerno, Manuela [Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, Istituto Ortopedico Rizzoli, Bologna (Italy); Hosogi, Shigekuni [Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, Istituto Ortopedico Rizzoli, Bologna (Italy); Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto (Japan); Kusuzaki, Katsuyuki [Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto (Japan); Baldini, Nicola [Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, Istituto Ortopedico Rizzoli, Bologna (Italy); Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna (Italy)

    2014-01-01

    Malignant tumors show intense glycolysis and, as a consequence, high lactate production and proton efflux activity. We investigated proton dynamics in osteosarcoma, rhabdomyosarcoma, and chondrosarcoma, and evaluated the effects of esomeprazole as a therapeutic agent interfering with tumor acidic microenvironment. All sarcomas were able to survive in an acidic microenvironment (up to 5.9–6.0 pH) and abundant acidic lysosomes were found in all sarcoma subtypes. V-ATPase, a proton pump that acidifies intracellular compartments and transports protons across the plasma membrane, was detected in all cell types with a histotype-specific expression pattern. Esomeprazole administration interfered with proton compartmentalization in acidic organelles and induced a significant dose-dependent toxicity. Among the different histotypes, rhabdomyosarcoma, expressing the highest levels of V-ATPase and whose lysosomes are most acidic, was mostly susceptible to ESOM treatment. - Highlights: • Osteosarcoma, rhabdomyosarcoma, and chondrosarcoma survive in acidic microenvironment. • At acidic extracellular pH, sarcoma survival is dependent on V-ATPase expression. • Esomeprazole administration induce a significant dose-dependent toxicity.

  4. Tree species effect on the redistribution of soil metals

    International Nuclear Information System (INIS)

    Mertens, Jan; Van Nevel, Lotte; De Schrijver, An; Piesschaert, Frederic; Oosterbaan, Anne; Tack, Filip M.G.; Verheyen, Kris

    2007-01-01

    Phytostabilization of metals using trees is often promoted although the influence of different tree species on the mobilization of metals is not yet clear. Soil and biomass were sampled 33 years after planting four tree species (Quercus robur, Fraxinus excelsior, Acer pseudoplatanus, Populus 'Robusta') in a plot experiment on dredged sediment. Poplar took up high amounts of Cd and Zn and this was associated with increased Cd and Zn concentrations in the upper soil layer. The other species contained normal concentrations of Cd, Cu, Cr, Pb and Zn in their tissues. Oak acidified the soil more than the other species and caused a decrease in the concentration of metals in the upper soil layer. The pH under poplar was lower than expected and associated with high carbon concentrations in the top soil. This might be assigned to retardation of the litter decomposition due to elevated Cd and Zn concentrations in the litter. - Trees (33-year-old) growing on polluted dredged sediment have influenced the metal concentration in the upper soil layer and there was a significant tree species effect

  5. The Effect of Smartphones on Human Health Relative to User's Addiction: A Study on a Wide Range of Audiences in Jordan

    OpenAIRE

    T. Qasim; M. Obeidat; S. Al-Sharairi

    2017-01-01

    The objective of this study is to investigate the effect of the excessive use of smartphones. Smartphones have enormous effects on the human body in that some musculoskeletal disorders (MSDs) and health problems might evolve. These days, there is a wide use of the smartphones among all age groups of society, thus, the focus on smartphone effects on human behavior and health, especially on the young and elderly people, becomes a crucial issue. This study was conducted in Jordan on smartphone u...

  6. Effect of CaO2 addition on anaerobic digestion of waste activated sludge at different temperatures and the promotion of valuable carbon source production under ambient condition.

    Science.gov (United States)

    Ping, Qian; Lu, Xiao; Zheng, Ming; Li, Yongmei

    2018-06-06

    The effect of calcium peroxide (CaO 2 ) addition on anaerobic digestion (AD) of waste activated sludge (WAS) at different temperatures (20 °C, 35 °C, and 55 °C) were investigated. The results show that CaO 2 addition had significant positive effect on short-chain fatty acids (SCFAs) production under ambient and mesophilic conditions. Polysaccharides and proteins embedded in extracellular polymeric substances (EPS) were effectively released from inner fraction to outer fraction, and non-biodegradable humic-like substances were decreased while easily biodegradable tryptophan-like proteins increased. These effects were most remarkable under ambient conditions. However, CaO 2 addition was unfavorable to thermophilic AD because of high free ammonia concentrations and the accumulation of humic-like substances. Temperature showed a stronger effect than CaO 2 on microbial community structure, but CaO 2 addition was more effective than temperature in enhancing hydrolytic and acidifying microorganisms. Predictive functional profiling indicated that microbial hydrolysis, metabolism and acidification were promoted by CaO 2 under ambient conditions. Copyright © 2018 Elsevier Ltd. All rights reserved.

  7. The Effect of Acid pH Modifiers on the Release Characteristics of Weakly Basic Drug from Hydrophlilic–Lipophilic Matrices

    OpenAIRE

    Dvořáčková, Kateřina; Doležel, Petr; Mašková, Eliška; Muselík, Jan; Kejdušová, Martina; Vetchý, David

    2013-01-01

    The solubility of weakly basic drugs within passage though GI tract leads to pH-dependent or even incomplete release of these drugs from extended release formulations and consequently to lower drug absorption and bioavailability. The aim of the study was to prepare and evaluate hydrophilic–lipophilic (hypromellose–montanglycol wax) matrix tablets ensuring the pH-independent delivery of the weakly basic drug verapamil-hydrochloride by an incorporation of three organic acidifiers (citric, fumar...

  8. Evaluation of gaseous chlorine dioxide for the inactivation of Tulane virus on blueberries.

    Science.gov (United States)

    Kingsley, David H; Pérez-Pérez, Rafael E; Niemira, Brendan A; Fan, Xuetong

    2018-05-20

    To determine the effectiveness of gaseous chlorine dioxide (gClO 2 ) against a human norovirus surrogate on produce, gClO 2 was generated and applied to Tulane virus-coated blueberries in a 240 ml-treatment chamber. gClO 2 was produced by an acidifying sodium chlorite solution. Initial assessments indicated that blueberries treated with gClO 2 generated from ≤1 mg acidified sodium chlorite in the small chamber appeared unaffected while gClO 2 generated from ≥10 mg of acidified sodium chlorite solution altered the appearance and quality of the blueberries. Treatments of inoculated blueberries with gClO 2 generated from 0.1 mg sodium chlorite reduced the virus populations by >1 log after exposure for 30 to 330 min. For the 1 mg sodium chlorite treatments, the virus populations were reduced by >2.2 log after 15 min exposure and to non-detectable levels (>3.3 logs reductions) after 180 min exposure. Measured concentrations of gClO 2 peaked in the treatment chamber at 0.9 μg/l after 10 min for 0.1 mg treatments and 600 μg/l after around 20 min for 1 mg treatment. Overall results indicate that gClO 2 could be a feasible waterless intervention for blueberries and other produce. Published by Elsevier B.V.

  9. Increased fitness of a key appendicularian zooplankton species under warmer, acidified seawater conditions.

    Directory of Open Access Journals (Sweden)

    Jean-Marie Bouquet

    Full Text Available Ocean warming and acidification (OA may alter the fitness of species in marine pelagic ecosystems through community effects or direct physiological impacts. We used the zooplanktonic appendicularian, Oikopleura dioica, to assess temperature and pH effects at mesocosm and microcosm scales. In mesocosms, both OA and warming positively impacted O. dioica abundance over successive generations. In microcosms, the positive impact of OA, was observed to result from increased fecundity. In contrast, increased pH, observed for example during phytoplankton blooms, reduced fecundity. Oocyte fertility and juvenile development were equivalent under all pH conditions, indicating that the positive effect of lower pH on O. dioica abundance was principally due to increased egg number. This effect was influenced by food quantity and quality, supporting possible improved digestion and assimilation at lowered pH. Higher temperature resulted in more rapid growth, faster maturation and earlier reproduction. Thus, increased temperature and reduced pH had significant positive impacts on O. dioica fitness through increased fecundity and shortened generation time, suggesting that predicted future ocean conditions may favour this zooplankton species.

  10. Effect of simulated acid rain on vegetation

    Energy Technology Data Exchange (ETDEWEB)

    Ferenbaugh, R.W.

    1974-01-01

    Experiments were performed to determine the effects of simulated acid rain on Chenopodium quinoa, Hordeum vulgare and Phaseolus vulgaris. Detailed experiments were conducted only on Phaseolus vulgaris. Sulfuric acid solutions covering a pH range of 1.5 to 3.5 were used. Gross morphological effects noted at lower pH values included failure to attain normal height, necrosis and wrinkling of leaves, excessive and adventitious budding, and premature abscission of primary leaves. Histological effects included smaller cell size, a decreased amount of intercellular space, hypertrophied nuclei and nucleoli, and a reduction in the size of starch granules within the chloroplasts. Dry weight remained an approximately constant percentage of fresh weight, and chlorophyll analyses showed that both chlorophyll concentration and ratio of chlorophyll to chlorophyll also remained constant. Respirometer studies showed that respiration rate increased slightly and photosynthetic rate increased dramatically. Quantitative analyses indicated that carbohydrate content was reduced at low pH values. Root biomass was also reduced. Application of Congo red indicator solution to the acid treated tissue showed that it was being acidified to a pH of below 4. Experiments involving aspiration of control tissue in acid solutions suggest that the increase in photosynthetic rate and the decreases in carbohydrate content and root biomass were caused by an uncoupling of photophosphorylation of adenosine diphosphate. Uncoupling was probably caused by hydrogen ion interference with proton pumps associated with the electron transport chain in the light reactions of photosynthesis. 128 references. (MDF)

  11. Effects of simulated acid rain on vegetation

    Energy Technology Data Exchange (ETDEWEB)

    Ferenbaugh, R.W.

    1974-01-01

    Experiments were performed to determine the effects of simulated acid rain on Chenopodium quinoa, Hordeum vulgare and Phaseolus vulgaris. Because of differential species' susceptibility, detailed experiments were conducted only on Phaseolus vulgaris. Acid rain was simulated by spraying the plants with a hand-held atomizer. Sulfuric acid solutions covering a pH range of 1.5 to 3.5 in one half pH unit increments were used. Gross morphological effects noted at lower pH values included failure to attain normal height, necrosis and wrinkling of leaves, excessive and adventitious budding, and premature abscission of primary leaves. Histological effects included smaller cell size, a decreased amount of intercellular space, hypertrophied nuclei and nucleoli, and a reduction in the size of starch granules within the chloroplasts. Dry weight remained an approximately constant percentage of fresh weight, and chlorophyll analyses showed that both chlorophyll concentration and ratio of chlorophyll 'a' to chlorophyll 'b' also remained constant. Respirometer studies showed that, while respiration rate increased only slightly at low pH values, photosynthetic rate increased dramatically. Quantitative analyses indicated that carbohydrate content was reduced at low pH values, with starch content reduced much more than sugar content. Root biomass was also reduced at low pH values. Application of Congo red indicator solution to the acid treated tissue showed that it was being acidified to a pH of below 4. 114 references, 23 figures, 12 tables.

  12. Investigation of Antileishmanial Effect of Alcoholic Extract and Essential Oil of Medicinal Plant Leaf Black Alfalfa (Medicago Lupulina), on The Number of Clinical Isolates of Leishmania Major Promastigotes in Vitro

    OpenAIRE

    E Gharirvand Eskandari; M Doudi

    2016-01-01

    Introduction: Leishmaniasis has created enormous global health problems. Side effects, drug resistance and the lack of effective vaccines had led to the new effective compounds effective of plants. The aim of this study was to introduce a traditional medicinal plant called Black alfalfa (Medicago Lupulina) that can be used as a valuable resource against cutaneous leishmaniasis. Methods: In this experimental study, alcoholic extract was prepared by maceration and essential oil by distillat...

  13. Ocean acidification and calcifying reef organisms: a mesocosm investigation

    Science.gov (United States)

    Jokiel, P. L.; Rodgers, K. S.; Kuffner, I. B.; Andersson, A. J.; Cox, E. F.; MacKenzie, F. T.

    2008-09-01

    A long-term (10 months) controlled experiment was conducted to test the impact of increased partial pressure of carbon dioxide ( pCO2) on common calcifying coral reef organisms. The experiment was conducted in replicate continuous flow coral reef mesocosms flushed with unfiltered sea water from Kaneohe Bay, Oahu, Hawaii. Mesocosms were located in full sunlight and experienced diurnal and seasonal fluctuations in temperature and sea water chemistry characteristic of the adjacent reef flat. Treatment mesocosms were manipulated to simulate an increase in pCO2 to levels expected in this century [midday pCO2 levels exceeding control mesocosms by 365 ± 130 μatm (mean ± sd)]. Acidification had a profound impact on the development and growth of crustose coralline algae (CCA) populations. During the experiment, CCA developed 25% cover in the control mesocosms and only 4% in the acidified mesocosms, representing an 86% relative reduction. Free-living associations of CCA known as rhodoliths living in the control mesocosms grew at a rate of 0.6 g buoyant weight year-1 while those in the acidified experimental treatment decreased in weight at a rate of 0.9 g buoyant weight year-1, representing a 250% difference. CCA play an important role in the growth and stabilization of carbonate reefs, so future changes of this magnitude could greatly impact coral reefs throughout the world. Coral calcification decreased between 15% and 20% under acidified conditions. Linear extension decreased by 14% under acidified conditions in one experiment. Larvae of the coral Pocillopora damicornis were able to recruit under the acidified conditions. In addition, there was no significant difference in production of gametes by the coral Montipora capitata after 6 months of exposure to the treatments.

  14. Ocean acidification and calcifying reef organisms: A mesocosm investigation

    Science.gov (United States)

    Jokiel, P.L.; Rodgers, K.S.; Kuffner, I.B.; Andersson, A.J.; Cox, E.F.; MacKenzie, F.T.

    2008-01-01

    A long-term (10 months) controlled experiment was conducted to test the impact of increased partial pressure of carbon dioxide (pCO2) on common calcifying coral reef organisms. The experiment was conducted in replicate continuous flow coral reef mesocosms flushed with unfiltered sea water from Kaneohe Bay, Oahu, Hawaii. Mesocosms were located in full sunlight and experienced diurnal and seasonal fluctuations in temperature and sea water chemistry characteristic of the adjacent reef flat. Treatment mesocosms were manipulated to simulate an increase in pCO2 to levels expected in this century [midday pCO2 levels exceeding control mesocosms by 365 ?? 130 ??atm (mean ?? sd)]. Acidification had a profound impact on the development and growth of crustose coralline algae (CCA) populations. During the experiment, CCA developed 25% cover in the control mesocosms and only 4% in the acidified mesocosms, representing an 86% relative reduction. Free-living associations of CCA known as rhodoliths living in the control mesocosms grew at a rate of 0.6 g buoyant weight year-1 while those in the acidified experimental treatment decreased in weight at a rate of 0.9 g buoyant weight year-1, representing a 250% difference. CCA play an important role in the growth and stabilization of carbonate reefs, so future changes of this magnitude could greatly impact coral reefs throughout the world. Coral calcification decreased between 15% and 20% under acidified conditions. Linear extension decreased by 14% under acidified conditions in one experiment. Larvae of the coral Pocillopora damicornis were able to recruit under the acidified conditions. In addition, there was no significant difference in production of gametes by the coral Montipora capitata after 6 months of exposure to the treatments. ?? 2008 Springer-Verlag.

  15. Distribution of 14 elements from two solutions simulating Hanford HLW Tank 102-SY (acid-dissolved sludge and acidified supernate) on four cation exchange resins and five anion exchange resins having different functional groups

    International Nuclear Information System (INIS)

    Marsh, S.F.; Svitra, Z.V.; Bowen, S.M.

    1995-01-01

    As part of the Tank Waste Remediation System program at Los Alamos, we evaluated a series of cation exchange and anion exchange resins for their ability to remove hazardous components from radioactive high-level waste (HLW). The anion exchangers were Reillex TM HPQ, a polyvinyl pyridine resin, and four strong-base polystyrene resins having trimethyl, tri ethyl, tri propyl, and tributyl amine as their respective functional groups. The cation exchange resins included Amberlyst TM 15 and Amberlyst tM XN-1010 with sulfonic acid functionality, Duolite TM C-467 with phosphonic acid functionality, and poly functional Diphonix TM with di phosphonic acid, sulfonic acid, and carboxylic acid functionalities. We measured the distributions of 14 elements on these resins from solutions simulating acid-dissolved sludge (pH 0.6) and acidified supernate (pH 3.5) from underground storage tank 102-SY at the Hanford Reservation near Richland, Washington, USA. To these simulants, we added the appropriate radionuclides and used gamma spectrometry to measure fission products (Ce, Cs, Sr, Tc, and Y), actinides (U, Pu, and Am), and matrix elements (Cr, Co, Fe, Mn, Zn, and Zr). For each of the 252 element/resin/solution combinations, distribution coefficients (Kds) were measured for dynamic contact periods of 30 minutes, 2 hours, and 6 hours to obtain information about sorption kinetics from these complex media. Because we measured the sorption of many different elements, the tabulated results indicate which unwanted elements are most likely to interfere with the sorption of elements of special interest. On the basis of these 756 measured Kd values, we conclude that some of the tested resins appear suitable for partitioning hazardous components from Hanford HLW. (author). 10 refs., 11 tabs

  16. Effect of gum tragacanth exuded by three Iranian Astragalus on mixed milk protein system during acid gelation.

    Science.gov (United States)

    Nejatian, Mohammad; Hatami, Masoud; Mohammadifar, Mohammad Amin

    2013-02-01

    The effects of various concentrations of three species of gum tragacanth on the gelation process, microstructure and viscoelastic properties of milk protein mixed gels acidified at 37°C by glucono-δ-lactone (GDL) were investigated using dynamic rheometry and microscopy. According to rheological measurements, the addition of gum tragacanth in the range of 0.05-0.2% (w/w) into milk protein dispersions led to a weaker structure for the milk protein network, compared to the control sample. This weakening effect could be eliminated by adding 0.3% (w/w) gum tragacanth exudates from A. gossypinus; the compositional features of gum tragacanth may have been responsible for the improved protein-protein interactions, greater structural strength and reduced gelation time onset. It was determined by scanning electron microscopy that the addition of gum tragacanth at a low concentration caused the density of the matrix to increase, while an open structure was observed in the presence of a higher gum concentration. Copyright © 2012 Elsevier B.V. All rights reserved.

  17. Effect of liming on the behaviour of 90Sr and 137Cs in a lake ecosystem

    International Nuclear Information System (INIS)

    Outola, Iisa; Rask, Martti

    2011-01-01

    Liming of lakes is considered one possible remedial action to reduce the accumulation of radionuclides into fish in the case of a radiological accident. These responses were tested in field conditions in a small acidified lake that was divided into two parts: one limed with CaCO 3 and the other half left as an unlimed control. The transfer of 90 Sr from water into fish decreased on average by 50% during the first year after liming. However, at the same time the 90 Sr concentration in water increased, reaching a maximum within 6 months after liming. Approximately 50% more 90 Sr was detected in water in the limed part of the lake than on control side during the first year. 90 Sr was most probably released from the sediment as the Ca concentration and pH of the water increased. As a result of these two processes, which counterbalanced each other (increased release of 90 Sr into water from sediment and decreased transfer of 90 Sr from water into fish), the 90 Sr concentration in fish did not notably differ between the limed and control sides of the lake. Liming may only be suitable as a remedial action if carried out immediately after a radiological accident, before significant amounts of radionuclides have been deposited in lake sediments. In the case of 137 Cs, the effect of liming was less pronounced. 137 Cs activity concentration in water increased in the first year by 20% and uptake by fish decreased by 20%. - Highlights: → Acidified lake was divided into two parts: one limed with CaCO 3 and the other half left as an unlimed control. → The transfer of 90 Sr from water into fish decreased by 50% during the first year after liming. → At the same time liming increased the 90 Sr concentration in the water by 50%. → The increased amount of 90 Sr in water is assumed to have originated from the lake sediments. → The 90 Sr concentration in fish remained relatively unchanged.

  18. Technology of uranium purification

    International Nuclear Information System (INIS)

    Abrao, A.

    1976-01-01

    The extraction of uranyl chloride by tri-n-octylamine (TOA) and its decontamination from several impurities with the aid of thiourea is investigated. The effect of thiourea as a complexing agent, giving rise to unextracted cationic species of several metallic ion and its consequence in the extraction of uranyl chloride from hydrochloriic acid with TOA-benzene (xilene) is described. Also, the effect of an acidified thiourea solution for scrubbing the organic phase loaded with uranyl chloride is studied [pt

  19. Effect of a fermented milk containing Bifidobacterium lactis DN-173010 on Chinese constipated women.

    Science.gov (United States)

    Yang, Yue-Xin; He, Mei; Hu, Gang; Wei, Jie; Pages, Philippe; Yang, Xian-Hua; Bourdu-Naturel, Sophie

    2008-10-28

    To investigate the effect of a fermented milk containing Bifidobacterium lactis DN-173010 and yogurt strains (BIO(R)) on adult women with constipation in Beijing. A total of 135 adult females with constipation were randomly allocated to consume for 2 wk either 100 g of the test fermented milk or 100 g of an acidified milk containing non-living bacteria (control). Stool frequency, defecation condition scores, stool consistency and food intake were recorded at baseline and after 1 and 2 wk in an intention-to-treat population of 126 subjects. In parallel, safety evaluation parameters were performed. At baseline, no differences were found between groups. Following consumption of test product, stool frequency was significantly increased after 1 wk (3.5 +/- 1.5 vs 2.4 +/- 0.6, P food intake did not change between the two groups, and safety parameters of the subjects were within normal ranges. This study suggests a beneficial effect of a fermented milk containing B. lactis DN-173010 on stool frequency, defecation condition and stool consistency in adult women with constipation constipated women after 1 and 2 wk of consumption.

  20. Arsenic exposure induces the Warburg effect in cultured human cells

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Fei; Severson, Paul; Pacheco, Samantha; Futscher, Bernard W.; Klimecki, Walter T., E-mail: klimecki@pharmacy.arizona.edu

    2013-08-15

    Understanding how arsenic exacts its diverse, global disease burden is hampered by a limited understanding of the particular biological pathways that are disrupted by arsenic and underlie pathogenesis. A reductionist view would predict that a small number of basic pathways are generally perturbed by arsenic, and manifest as diverse diseases. Following an initial observation that arsenite-exposed cells in culture acidify their media more rapidly than control cells, the report here shows that low level exposure to arsenite (75 ppb) is sufficient to induce aerobic glycolysis (the Warburg effect) as a generalized phenomenon in cultured human primary cells and cell lines. Expanded studies in one such cell line, the non-malignant pulmonary epithelial line, BEAS-2B, established that the arsenite-induced Warburg effect was associated with increased accumulation of intracellular and extracellular lactate, an increased rate of extracellular acidification, and inhibition by the non-metabolized glucose analog, 2-deoxy-D-glucose. Associated with the induction of aerobic glycolysis was a pathway-wide induction of glycolysis gene expression, as well as protein accumulation of an established glycolysis master-regulator, hypoxia-inducible factor 1A. Arsenite-induced alteration of energy production in human cells represents the type of fundamental perturbation that could extend to many tissue targets and diseases. - Highlights: • Chronic arsenite exposure induces aerobic glycolysis, dubbed the “Warburg effect”. • Arsenite-induced Warburg effect is a general phenomenon in cultured human cells. • HIF-1A may mediate arsenite induced Warburg effect.

  1. Arsenic exposure induces the Warburg effect in cultured human cells

    International Nuclear Information System (INIS)

    Zhao, Fei; Severson, Paul; Pacheco, Samantha; Futscher, Bernard W.; Klimecki, Walter T.

    2013-01-01

    Understanding how arsenic exacts its diverse, global disease burden is hampered by a limited understanding of the particular biological pathways that are disrupted by arsenic and underlie pathogenesis. A reductionist view would predict that a small number of basic pathways are generally perturbed by arsenic, and manifest as diverse diseases. Following an initial observation that arsenite-exposed cells in culture acidify their media more rapidly than control cells, the report here shows that low level exposure to arsenite (75 ppb) is sufficient to induce aerobic glycolysis (the Warburg effect) as a generalized phenomenon in cultured human primary cells and cell lines. Expanded studies in one such cell line, the non-malignant pulmonary epithelial line, BEAS-2B, established that the arsenite-induced Warburg effect was associated with increased accumulation of intracellular and extracellular lactate, an increased rate of extracellular acidification, and inhibition by the non-metabolized glucose analog, 2-deoxy-D-glucose. Associated with the induction of aerobic glycolysis was a pathway-wide induction of glycolysis gene expression, as well as protein accumulation of an established glycolysis master-regulator, hypoxia-inducible factor 1A. Arsenite-induced alteration of energy production in human cells represents the type of fundamental perturbation that could extend to many tissue targets and diseases. - Highlights: • Chronic arsenite exposure induces aerobic glycolysis, dubbed the “Warburg effect”. • Arsenite-induced Warburg effect is a general phenomenon in cultured human cells. • HIF-1A may mediate arsenite induced Warburg effect

  2. Update: Health Status of Iranian Victims of Chemical Weapons / Ongoing Research Projects Addressing CW Health Effects in Iran

    International Nuclear Information System (INIS)

    Khateri, S.

    2007-01-01

    Use of chemical weapons against Iran during the 1980s was a horrifying epic in the annals of modern warfare, inflicting enormous suffering during the conflict that continues to the present day in the form of latent illness among survivors. Surviving victims suffer from a diverse range of chronic illnesses placing an enormous strain on the nation's medical infrastructure. To define the scope of this problem, the National Organization for Veteran's Affairs (Janbazan) established a subsidiary research department called Janbazan Medical and Engineering Research Center (JMERC). Beginning in 2000 JMERC has conducted epidemiological, clinical and basic scientific studies to characterize disease among chemical attack survivors and develop new therapeutic strategies. The primary JMERC mission has been to identify where resources may be allocated so as to most effectively treat patients with the greatest need - requiring a comprehensive picture of the major medical problems among this population. Accordingly, JMERC's initial task was to define the nature and distribution of serious chronic illness among CW survivors. Therefore epidemiological studies in CW-exposed Iranian populations are currently underway. Ultimately these studies will allow management of illness among CW-exposed populations that is both compassionate and cost-effective. A summary of the above mentioned research projects will be reported in this article. (author)

  3. Tracing of shading effect on underachieving SPV cell of an SPV grid using wireless sensor network

    OpenAIRE

    Kaundal, Vivek; Mondal, Amit Kumar; Sharma, Paawan; Bansal, Kamal

    2015-01-01

    The environmental and economic merits of converting solar energy into electricity via photovoltaic cells have led to its enormous growth in this sector. Besides material and design parameters, there are many other factors which locally affect Photovoltaic cell like partial shading, humidity, dust, bird droppings, air velocity etc. However, the effect due to a single solar photo voltaic cell being connected to a serial or parallel network (to form a grid) has never been deliberated extensively...

  4. Long term effects of ash recycling on soil and water chemistry in forests

    International Nuclear Information System (INIS)

    Westling, Olle; Kronnaes, Veronika

    2006-02-01

    IVL Swedish Environmental Research Institute has studied the long-term need of compensatory fertilisation (e.g. wood ash recycling) after whole tree harvest in coniferous forests in Sweden. The study is based on dynamic model calculations with scenarios including reduced atmospheric deposition of air pollutants and different intensity of forest management. The possibilities to counteract acidification in soil and water with application of stabilised wood ash are discussed. The reduction in deposition of acidifying air pollutants in Sweden up to 2010 is expected to contribute to a significant recovery from acidification in soil- and runoff water in forests. The recovery of the forest soil (e.g. base saturation ) will, however, be slow according to the model calculations, especially if compensatory fertilisation is not carried out in managed areas. The model calculations indicate that the harvest of stemwood will have limited impact on the future acidity of soil and run off water from well drained forest soils. This conclusion is based on a comparison with a scenario where no harvest is assumed. More important for recovery from acidification is further reduction of acidifying air pollutants, even after 2010. Harvest of stemwood in combination with extraction of harvest residues has the potential to cause significant and long term acidification of soils in the future, especially in areas with high forest production and slow weathering rate. The results of the study indicate a need of compensatory fertilisation in these areas if whole tree harvest is applied, especially if the deposition of air pollutants have been high in the past. Field studies have shown that acidification effects of whole tree harvest can be counteracted by wood ash recycling to forest soils, due to the high content of calcium- and magnesium-rich minerals in the ashes. However, the dose should be adjusted to the need of increasing the acid neutralising capacity in the soil and runoff and the actual

  5. Environmental pollution: An enormous and invisible burden on health systems in low- and middle-income counties.

    Science.gov (United States)

    Landrigan, Philip J; Fuller, Richard

    2014-01-01

    Background. Environmental pollution has become the leading risk factor for death in low- and middle-income countries (LMICs). The World Health Organization and others calculate that exposures to polluted air - indoor and outdoor, water and soil resulted in 8.4 million deaths in LMICs in 2012. By comparison, HIV/AIDS causes 1.5 million deaths per year, and malaria and tuberculosis Less than 1 million each. The diseases caused by pollution include the traditional scourges of pneumonia and diarrhea, but increasingly they also include chronic, non-communicable diseases (NCDs) such as such as heart disease, stroke and cancer. Method. We review the diseases caused by pollution and the multiple economic and human burdens that these diseases impose on health systems in countries with already limited resources. Results. We find that diseases caused by pollution increase health care costs, especially for high-cost NCDs. They impose an unnecessary load on health care delivery systems by increasing hospital staffing needs and thus diverting resources from essential prevention programmes such as childhood immunizations, infection control and maternal and child health. They undermine the development of poor countries by reducing the health, intelligence and economic productivity of entire generations. Pollution is highly preventable and pollution prevention is highly cost-effective. Yet despite their high economic and human costs and amenability to prevention, the diseases caused by pollution have not received the attention that they deserve in policy planning or in the international development agenda. Conclusion. Pollution is not inevitable. It is a problem that can be solved in our lifetime. Given the great impact of pollution on health and health care resources and the high cost-benefit ratio of pollution prevention, efforts to mitigate pollution should become a key strategic priority for international funders and for governments of LMICs. Recommendation. Assisting LMICs to

  6. Effect of the soil type on the microbiome in the rhizosphere of field-grown lettuce

    OpenAIRE

    Schreiter, Susanne; Ding, Guo-Chun; Heuer, Holger; Neumann, Günter; Sandmann, Martin; Grosch, Rita; Kropf, Siegfried; Smalla, Kornelia

    2014-01-01

    The complex and enormous diversity of microorganisms associated with plant roots is important for plant health and growth and is shaped by numerous factors. This study aimed to unravel the effects of the soil type on bacterial communities in the rhizosphere of field-grown lettuce. We used an experimental plot system with three different soil types that were stored at the same site for 10 years under the same agricultural management to reveal differences directly linked to the soil type and no...

  7. Effect of the soil type on the microbiome in the rhizosphere of field-grown lettuce

    OpenAIRE

    Susanne eSchreiter; Susanne eSchreiter; Guo-chun eDing; Guo-chun eDing; Holger eHeuer; Günter eNeumann; Martin eSandmann; Rita eGrosch; Siegfried eKropf; Kornelia eSmalla

    2014-01-01

    The complex and enormous diversity of microorganisms associated with plant roots is important for plant health and growth and is shaped by numerous factors. This study aimed to unravel the effects of the soil type on bacterial communities in the rhizosphere of field-grown lettuce. We used an experimental plot system with three different soil types that were stored at the same site for ten years under the same agricultural management to reveal differences directly linked to the soil type and n...

  8. Month of the Year Effect Pada Beberapa Pasar Modal di Asia Tenggara dan Pasar Komoditas

    OpenAIRE

    Robiyanto Robiyanto

    2016-01-01

    One of prominent phenomenon in capital market is month of the year effect which is the occurence of certain monthly pattern in capital market return during trading years. There were enormous researches, which had been done to explain this phenomenon in capital market but the results always varied. Unfortunately there was a few research to explain this phenomenon in commodities market. Based on these facts, research about this seasonality still need to be done both in capital market and commod...

  9. Rehabilitation of acidified and eutrophied fens in The Netherlands : Effects of hydrologic manipulation and liming

    NARCIS (Netherlands)

    Beltman, B.; Broek, T. van den; Barendregt, A.; Bootsma, M.C.; Grootjans, A.P.

    Mosaic fen communities with many rare plant species were outcompeted by fast-growing bryophyte stands (Sphagnum flexuosum/Sphagnum fallax and Polytrichum commune). Eutrophication and acidification were the main causes. Management experiments were carried out to set back this succession by restoring

  10. 21 CFR 131.162 - Acidified sour cream.

    Science.gov (United States)

    2010-04-01

    ... HUMAN CONSUMPTION MILK AND CREAM Requirements for Specific Standardized Milk and Cream § 131.162.... (c) Methods of analysis. Referenced methods in paragraphs (c) (1) and (2) of this section are from “Official Methods of Analysis of the Association of Official Analytical Chemists,” 13th Ed. (1980), which is...

  11. Odors from evaporation of acidified pig urine

    NARCIS (Netherlands)

    Willers, H.C.; Hobbs, P.J.; Ogink, N.W.M.

    2004-01-01

    In the Dutch Hercules project feces and urine from pigs are collected separately underneath the slatted floor in a pig house and treated in two processes. Feces are composted and urine is concentrated by water evaporation in a packed bed. Exhaust air from the pig house is used for the evaporation in

  12. The Financing of the London Olympics 2012 and its economic effects on the U.K.

    OpenAIRE

    Khandelwal, Mohak

    2010-01-01

    The Olympics are being seen as an event that can turn an ordinary city into a world city. The past has shown that hosting the games can make a country have enormous profits or, on the other hand, go into deep losses, and the enormous amounts of money invested in them are evidence of this. This paper aims to examine The United Kingdom’s preparations for the 2012 Olympic Games by focussing on the various costs that are incurred, in particular on the plans for a positive economic legacy, and fin...

  13. Effects of Fertilization on Uptake of 85Sr, 60Co and 54Mn by Tomato and Phaseolus Plants

    International Nuclear Information System (INIS)

    Ramadan, A.B.; Ezz El-Din, M.R.

    2001-01-01

    The effects of N-, P-, and K- fertilizers on availability of 85 Sr, 60 Co and 54 Mn added to the soil were measured in an open field experiment. The uptake of 85 Sr, 60 Co and 54 Mn by tomato and phaseolus was lower in fertilized treatments than in unfertilized ones. The radionuclide availability under fertilized condition depends on soil and element properties. Solubilization of Ca-ions following nitrification of nitrogen in ammonium salts and the presence of stable Strontium, Cobalt and Manganese in the acidifying fertilizers are the main factors giving rise to the reduced radioisotopes uptake by plants. The relative order of uptake of the investigated radionuclides by plants appeared to be as follow 54 Mn> 60 Co> 85 Sr. The distribution pattern of the total absorbed radionuclides in the two plants shows that the shoots contained the highest percent of these radionuclides. Transfer factors for phaseolus plants were higher than those of tomato plants

  14. Effects of biochar, waste water irrigation and fertilization on soil properties in West African urban agriculture.

    Science.gov (United States)

    Häring, Volker; Manka'abusi, Delphine; Akoto-Danso, Edmund K; Werner, Steffen; Atiah, Kofi; Steiner, Christoph; Lompo, Désiré J P; Adiku, Samuel; Buerkert, Andreas; Marschner, Bernd

    2017-09-06

    In large areas of sub-Saharan Africa crop production must cope with low soil fertility. To increase soil fertility, the application of biochar (charred biomass) has been suggested. In urban areas, untreated waste water is widely used for irrigation because it is a nutrient-rich year-round water source. Uncertainty exists regarding the interactions between soil properties, biochar, waste water and fertilization over time. The aims of this study were to determine these interactions in two typical sandy, soil organic carbon (SOC) and nutrient depleted soils under urban vegetable production in Tamale (Ghana) and Ouagadougou (Burkina Faso) over two years. The addition of biochar at 2 kg m -2 made from rice husks and corn cobs initially doubled SOC stocks but SOC losses of 35% occurred thereafter. Both biochar types had no effect on soil pH, phosphorous availability and effective cation exchange capacity (CEC) but rice husk biochar retained nitrogen (N). Irrigation with domestic waste water increased soil pH and exchangeable sodium over time. Inorganic fertilization alone acidified soils, increased available phosphorous and decreased base saturation. Organic fertilization increased SOC, N and CEC. The results from both locations demonstrate that the effects of biochar and waste water were less pronounced than reported elsewhere.

  15. Base cation depletion, eutrophication and acidification of species-rich grasslands in response to long-term simulated nitrogen deposition

    Energy Technology Data Exchange (ETDEWEB)

    Horswill, Paul [Department of Animal and Plant Sciences, University of Sheffield, Alfred Denny Building, Western Bank, Sheffield S10 2TN (United Kingdom)], E-mail: paul.horswill@naturalengland.org.uk; O' Sullivan, Odhran; Phoenix, Gareth K.; Lee, John A.; Leake, Jonathan R. [Department of Animal and Plant Sciences, University of Sheffield, Alfred Denny Building, Western Bank, Sheffield S10 2TN (United Kingdom)

    2008-09-15

    Pollutant nitrogen deposition effects on soil and foliar element concentrations were investigated in acidic and limestone grasslands, located in one of the most nitrogen and acid rain polluted regions of the UK, using plots treated for 8-10 years with 35-140 kg N ha{sup -2} y{sup -1} as NH{sub 4}NO{sub 3}. Historic data suggests both grasslands have acidified over the past 50 years. Nitrogen deposition treatments caused the grassland soils to lose 23-35% of their total available bases (Ca, Mg, K, and Na) and they became acidified by 0.2-0.4 pH units. Aluminium, iron and manganese were mobilised and taken up by limestone grassland forbs and were translocated down the acid grassland soil. Mineral nitrogen availability increased in both grasslands and many species showed foliar N enrichment. This study provides the first definitive evidence that nitrogen deposition depletes base cations from grassland soils. The resulting acidification, metal mobilisation and eutrophication are implicated in driving floristic changes. - Nitrogen deposition causes base cation depletion, acidification and eutrophication of semi-natural grassland soils.

  16. Disentangling the effects of evolutionary, demographic, and environmental factors influencing genetic structure of natural populations: Atlantic herring as a case study

    DEFF Research Database (Denmark)

    Gaggiotti, Oscar E.; Bekkevold, Dorte; Jørgensen, Hanne B.H.

    2009-01-01

    , on the other hand, seems to have been largely eroded, which is not surprising given the large reproductive potential and presumed enormous local effective population sizes of pelagic fish that constrain the effect of stochastic processes. The approach we used can in principle be applied to any abundant...... carried out separate analyses of neutral and selected genetic variation, which allowed us to establish that the two most important factors affecting population structure were selection due to salinity at spawning sites and feeding migrations. The genetic signal left by the demographic history of herring...

  17. Protective effect of bovine milk against HCl and ethanol-induced gastric ulcer in mice.

    Science.gov (United States)

    Yoo, Jeong-Hyun; Lee, Jeong-Sang; Lee, You-Suk; Ku, SaeKwang; Lee, Hae-Jeung

    2018-05-01

    The purpose of this study was to investigate the gastroprotective effects of bovine milk on an acidified ethanol (HCl-ethanol) mixture that induced gastric ulcers in a mouse model. Mice received different doses of commercial fresh bovine milk (5, 10, and 20 mL/kg of body weight) by oral gavage once a day for 14 d. One hour after the last oral administration of bovine milk, the HCl-ethanol mixture was orally intubated to provoke severe gastric damage. Our results showed that pretreatment with bovine milk significantly suppressed the formation of gastric mucosa lesions. Pretreatment lowered gastric myeloperoxidase and increased gastric mucus contents and antioxidant enzymes catalase and superoxide dismutase. Administration of bovine milk increased nitrate/nitrite levels and decreased the malondialdehyde levels and the expression of proinflammatory genes, including transcription factor nuclear factor-κB, cyclooxygenase-2, and inducible nitric oxide synthase in the stomach of mice. These results suggest that bovine milk can prevent the development of gastric ulcer caused by acid and alcohol in mice. Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  18. Effects on the atmosphere of a major nuclear exchange

    International Nuclear Information System (INIS)

    1985-01-01

    The Committee on the Atmospheric Effects of Nuclear Explosions addressed the following charge: (1) determine the manner in which the atmosphere of the earth would be modified by a major exchange of nuclear weapons and, insofar as the current state of knowledge and understanding permits, give a quantitative description of the more important of the changes; and (2) recommend research and exploratory work appropriate to a better understanding of the question. Recent calculations by different investigators suggest that the climatic effects from a major nuclear exchange could be large in scale. Although there are enormous uncertainties involved in the calculations, the committee believes that long-term climatic effects with severe implications for the biosphere could occur, and these effects should be included in any analysis of the consequences of nuclear war. The estimates are necessarily rough and can only be used as a general indication of the seriousness of what might occur

  19. Association of metabolic acidosis with bovine milk-based human milk fortifiers.

    Science.gov (United States)

    Cibulskis, C C; Armbrecht, E S

    2015-02-01

    To compare the incidence of metabolic acidosis and feeding intolerance associated with powdered or acidified liquid human milk fortifier (HMF). This retrospective study evaluated infants ⩽ 32 weeks gestational age or ⩽ 1500 g birth weight who received human milk with either powdered or acidified liquid HMF (50 consecutively born infants per group). Primary outcomes tracked were metabolic acidosis (base excess less than -4 mmol l(-1) or bicarbonate less than 18 mmol l(-1)), feeding intolerance (gastric residual > 50% feed volume, > 3 loose stools or emesis per day, abdominal tenderness or distention), necrotizing enterocolitis, late-onset infection, death, length of hospital stay and ability to remain on HMF. Demographics, feeding practices, growth parameters and laboratory data were also collected. Significantly more infants who received acidified liquid HMF developed metabolic acidosis (P acidosis or feeding intolerance than those on powdered HMF (P acidosis and to be switched off HMF than those who received powdered HMF. Growth in the liquid HMF group was no different than the powdered group, despite higher protein intake.

  20. Machine characteristics, system arrangement, driver and operation effects on surge of dynamic compressor in oil and gas plants

    Energy Technology Data Exchange (ETDEWEB)

    Almasi, Amin

    2012-12-15

    Working in the surge area will result in an unstable compressor operation, exposing the dynamic compressor (centrifugal compressor or axial compressor) to destructive stress, high vibration and other damaging effects. The destructive power of the surge is enormous, ranging from changes in clearances, which result in a penalty in the compressor efficiency, to destruction of parts leading to bearing, rotor or seal replacements. The effects of compressor characteristics, driver type, compressor accessories, vent valve, check valve, trip delay and operation details on surge events and anti-surge system designs are studied. A case study is also discussed. (orig.)

  1. Effects of Physical Exercise on Cognitive Functioning and Wellbeing: Biological and Psychological Benefits

    Directory of Open Access Journals (Sweden)

    Laura Mandolesi

    2018-04-01

    Full Text Available Much evidence shows that physical exercise (PE is a strong gene modulator that induces structural and functional changes in the brain, determining enormous benefit on both cognitive functioning and wellbeing. PE is also a protective factor for neurodegeneration. However, it is unclear if such protection is granted through modifications to the biological mechanisms underlying neurodegeneration or through better compensation against attacks. This concise review addresses the biological and psychological positive effects of PE describing the results obtained on brain plasticity and epigenetic mechanisms in animal and human studies, in order to clarify how to maximize the positive effects of PE while avoiding negative consequences, as in the case of exercise addiction.

  2. Noncovalent Hydrogen Isotope Effects

    Science.gov (United States)

    Buchachenko, A. L.; Breslavskaya, N. N.

    2018-02-01

    Zero-point energies (ZPE) and isotope effects, induced by intermolecular, noncovalent vibrations, are computed and tested by experimental data. The ZPE differences of H- and D-complexes of water with hydrogen, methane, and water molecules are about 100-300 cal/mol; they result to isotope effects IE of 1.20-1.70. Semi-ionic bonds between metal ions and water ligands in M(H2O) 6 2+ complexes are much stronger; their ZPEs are about 12-14 kcal/mol per molecule and result to IE of 1.9-2.1 at 300 K. Protonated (deuterated) water and biwater exhibit the largest ZPE differences and isotope effects; the latter are 25-28 and 12-13 for water and biwater, respectively. Noncovalent IEs contribute markedly into the experimentally measured effects and explain many anomalous and even magic properties of the effects, such as the dependence of IE on the solvents and on the presence of the third substances, enormously large isotope effects at the mild conditions, the difference between IEs measured in the reactions of individual protiated and deuterated compounds and those measured in their mixture. Noncovalent IEs are not negligible and should be taken into account to make correct and substantiated conclusions on the reaction mechanisms. The kinetic equations are derived for the total isotope effects, which include noncovalent IEs as additive factors.

  3. The effects of calcium regulation of endosperm reserve protein ...

    African Journals Online (AJOL)

    Administrator

    2011-06-15

    Jun 15, 2011 ... on barley endosperm protein mobilization during malting. Although, the site and ... fractionating head of the digesting vigreux column. The digest was ... growth and enormous reductions in malting loss (Ezeogu and Okolo ...

  4. The mastermind approach to CNS drug therapy: translational prediction of human brain distribution, target site kinetics, and therapeutic effects

    OpenAIRE

    de Lange, Elizabeth CM

    2013-01-01

    Despite enormous advances in CNS research, CNS disorders remain the world?s leading cause of disability. This accounts for more hospitalizations and prolonged care than almost all other diseases combined, and indicates a high unmet need for good CNS drugs and drug therapies. Following dosing, not only the chemical properties of the drug and blood?brain barrier (BBB) transport, but also many other processes will ultimately determine brain target site kinetics and consequently the CNS effects. ...

  5. Benthic infaunal community structuring in an acidified tropical estuarine system.

    Science.gov (United States)

    Hossain, M Belal; Marshall, David J

    2014-01-01

    Recent studies suggest that increasing ocean acidification (OA) should have strong direct and indirect influences on marine invertebrates. While most theory and application for OA is based on relatively physically-stable oceanic ecological systems, less is known about the effects of acidification on nearshore and estuarine systems. Here, we investigated the structuring of a benthic infaunal community in a tropical estuarine system, along a steep salinity and pH gradient, arising largely from acid-sulphate groundwater inflows (Sungai Brunei Estuary, Borneo, July 2011- June 2012). Preliminary data indicate that sediment pore-water salinity (range: 8.07 - 29.6 psu) declined towards the mainland in correspondence with the above-sediment estuarine water salinity (range: 3.58 - 31.2 psu), whereas the pore-water pH (range: 6.47- 7.72) was generally lower and less variable than the estuarine water pH (range: 5.78- 8.3), along the estuary. Of the thirty six species (taxa) recorded, the polychaetes Neanthes sp., Onuphis conchylega, Nereididae sp. and the amphipod Corophiidae sp., were numerically dominant. Calcified microcrustaceans (e.g., Cyclopoida sp. and Corophiidae sp.) were abundant at all stations and there was no clear distinction in distribution pattern along the estuarine between calcified and non-calcified groups. Species richness increased seawards, though abundance (density) showed no distinct directional trend. Diversity indices were generally positively correlated (Spearman's rank correlation) with salinity and pH (p 0.05). Three faunistic assemblages were distinguished: (1) nereid-cyclopoid-sabellid, (2) corophiid-capitellid and (3) onuphid- nereid-capitellid. These respectively associated with lower salinity/pH and a muddy bottom, low salinity/pH and a sandy bottom, and high salinity/pH and a sandy bottom. However, CCA suggested that species distribution and community structuring is more strongly influenced by sediment particle characteristics than by the

  6. Acceleration of pH variation in cloudy apple juice using electrodialysis with bipolar membranes.

    Science.gov (United States)

    Lam Quoc, A; Lamarche, F; Makhlouf, J

    2000-06-01

    The purpose of this study was to accelerate pH variation in cloudy apple juice using electrodialysis (ED). The testing was conducted using two ED configurations. The bipolar and cationic membrane configuration showed that reducing the spacing from 8 to 0.75 mm had little effect on treatment time, whereas stacking eight bipolar membranes reduced acidification time by 30%, although the treatment still took too long (21 min). Furthermore, it was not possible to acidify apple juice to a pH of 2.0 to completely inhibit enzymatic browning. The bipolar and anionic membrane configuration helped to accelerate the acidification step by a factor of 3, increasing the yield from 3.3 to 10 L of juice/m(2) membrane/min. Moreover, treatment time was inversely proportional to the size of the membrane stack. The speed at which the pH of acidified juice returned to its initial value was, however, 4 times slower than the speed of acidification, giving a yield of 2.5 L of juice/m(2) membrane/min. By accelerating the acidification step, ED treatment with bipolar and anionic membranes results in more effective polyphenol oxidase activity and more rapid control of juice browning at pH 2.0. Also, the treatment has very little effect on the chemical composition and organoleptic quality of apple juice.

  7. Partitioning of metals in a degraded acid sulfate soil landscape: influence of tidal re-inundation.

    Science.gov (United States)

    Claff, Salirian R; Sullivan, Leigh A; Burton, Edward D; Bush, Richard T; Johnston, Scott G

    2011-11-01

    The oxidation and acidification of sulfidic soil materials results in the re-partitioning of metals, generally to more mobile forms. In this study, we examine the partitioning of Fe, Cr, Cu, Mn, Ni and Zn in the acidified surface soil (0-0.1 m) and the unoxidised sub-soil materials (1.3-1.5 m) of an acid sulfate soil landscape. Metal partitioning at this acidic site was then compared to an adjacent site that was previously acidified, but has since been remediated by tidal re-inundation. Differences in metal partitioning were determined using an optimised six-step sequential extraction procedure which targets the "labile", "acid-soluble", "organic", "crystalline oxide", "pyritic" and "residual" fractions. The surficial soil materials of the acidic site had experienced considerable losses of Cr, Cu, Mn and Ni compared to the underlying parent material due to oxidation and acidification, yet only minor losses of Fe and Zn. In general, the metals most depleted from the acidified surface soil materials exhibited the greatest sequestration in the surface soil materials of the tidally remediated site. An exception to this was iron, which accumulated to highly elevated concentrations in the surficial soil materials of the tidally remediated site. The "acid-soluble", "organic" and "pyritic" fractions displayed the greatest increase in metals following tidal remediation. This study demonstrates that prolonged tidal re-inundation of severely acidified acid sulfate soil landscapes leads to the immobilisation of trace metals through the surficial accumulation of iron oxides, organic material and pyrite. Copyright © 2011 Elsevier Ltd. All rights reserved.

  8. Calcium, snails, and birds: a case study

    Directory of Open Access Journals (Sweden)

    R. Mänd

    2000-10-01

    Full Text Available Recent studies have shown that wild birds breeding in acidified areas have difficulties with obtaining sufficient calcium for their eggshells, and that the cause of it is the shortage of land snails. Many birds have to search for Ca-rich snail shells on a daily basis during egg production. Molluscs depend on litter calcium, which has decreased due to acidification of the environment. Calcium limitation may be a widespread phenomenon also in non-acidified, naturally Ca-poor areas. The problem is that while in the latter areas the time for development of specific adaptations may have been sufficient, then in acidified areas, on the contrary, calcium shortage is a recent phenomenon. Therefore, since the extent of calcium limitation in non-acidified areas is hard to derive from observational data, experimental approach is needed. We provide experimental evidence that specific calcium deficit does affect reproductive traits also in the birds breeding in naturally base-poor habitats. Our study was conducted in a heterogeneous woodland area in Estonia containing deciduous forest patches as well as base-poor pine forest with low snail abundance. Ca supplementation, using snail shell and chicken eggshell fragments, was carried out for pied flycatchers and great tits. Extra calcium affected positively several reproductive traits like egg volume and eggshell thickness, start of breeding, and fledglings’ parameters. The negative relationship between calcium availability and lay-date suggests that birds adjust their breeding tactics to conditions of Ca deficiency, for example, by postponing laying.

  9. Comparison of bio-dissolution of spent Ni-Cd batteries by sewage sludge using ferrous ions and elemental sulfur as substrate.

    Science.gov (United States)

    Zhao, Ling; Zhu, Nan-Wen; Wang, Xiao-Hui

    2008-01-01

    Bioleaching of spent Ni-Cd batteries using acidified sewage sludge was carried out in a continuous flow two-step leaching system including an acidifying reactor and a leaching reactor. Two systems operated about 30d to achieve almost complete dissolution of heavy metals Ni, Cd and Co in four Ni-Cd batteries. Ferrous sulphate and elemental sulfur were used as two different substrates to culture indigenous thiobacilli in sewage sludge. pH and ORP of the acidifying reactor was stabilized around 2.3 and 334mV for the iron-oxidizing system and 1.2 and 390mV for the sulfur-oxidizing system. It was opposite to the acidifying reactor, the pH/ORP in the leaching reactor of the iron-oxidizing system was relatively lower/higher than that of the sulphur-oxidizing system in the first 17d. The metal dissolution, in the first 12-16d, was faster in the iron-oxidizing system than in the sulphur-oxidizing system due to the lower pH. In the iron-oxidizing system, the maximum solubilization of cadmium (2500mg l(-1)) and cobalt (260mg l(-1)) can be reached at day 6-8 and the most of metal nickel was leached in the first 16d. But in the sulphur-oxidizing system there was a lag period of 4-8d to reach the maximum solubilization of cadmium and cobalt. The maximum dissolution of nickel hydroxide (1400mg l(-1)) and metallic nickel (2300mg l(-1)) occurred at about day 12 and day 20, respectively.

  10. Effect of Paris polyphylla extract on second-degree burns in rats

    African Journals Online (AJOL)

    International Pharmaceutical Abstract, Chemical Abstracts, Embase, Index Copernicus, EBSCO, African. Index Medicus ... accidents, which along with enormous cost of treatment, cause .... probably accelerated the process of wound healing ...

  11. Simulating CO₂ leakages from CCS to determine Zn toxicity using the marine microalgae Pleurochrysis roscoffensis.

    Science.gov (United States)

    Bautista-Chamizo, Esther; De Orte, Manoela Romanó; DelValls, Tomás Ángel; Riba, Inmaculada

    2016-02-01

    Due to the current climate change and ocean acidification, a new technology for CO2 mitigation has been proposed, the Carbon dioxide Capture and Storage (CCS). However, there is an ecological risk associated with potential CO2 leakages from the sub-seabed storages sites. To evaluate the effects related to CO2 leakages, laboratory-scales experiments were performed using the marine microalgae Pleurochrysis roscoffensis. Five Zn concentrations were tested at different pHs to study Zn toxicity under acidified conditions. Seawater was collected and submitted to acidification by means of CO2 injection and by HCl addition. Results showed differences between both acidification techniques: while microalgae growth was enhanced by CO2 supply, reaching the optimal growth at pH 6.5 and full inhibition at pH 5.5, HCl acidification growth was inhibited at pH 6.5. Although small concentrations of Zn were positive for P. roscoffensis growth, Zn toxicity increased at lower pHs, and more severely on samples acidified with HCl. The conclusions obtained in this work are useful to address the potential effects on the marine ecosystem related to changes in metal bioavailability during CO2 leakages scenarios. Copyright © 2015 Elsevier Ltd. All rights reserved.

  12. Differential tolerances to ocean acidification by parasites that share the same host.

    Science.gov (United States)

    MacLeod, C D; Poulin, R

    2015-06-01

    Ocean acidification is predicted to cause major changes in marine ecosystem structure and function over the next century, as species-specific tolerances to acidified seawater may alter previously stable relationships between coexisting organisms. Such differential tolerances could affect marine host-parasite associations, as either host or parasite may prove more susceptible to the stressors associated with ocean acidification. Despite their important role in many ecological processes, parasites have not been studied in the context of ocean acidification. We tested the effects of low pH seawater on the cercariae and, where possible, the metacercariae of four species of marine trematode parasite. Acidified seawater (pH 7.6 and 7.4, 12.5 °C) caused a 40-60% reduction in cercarial longevity and a 0-78% reduction in metacercarial survival. However, the reduction in longevity and survival varied distinctly between parasite taxa, indicating that the effects of reduced pH may be species-specific. These results suggest that ocean acidification has the potential to reduce the transmission success of many trematode species, decrease parasite abundance and alter the fundamental regulatory role of multi-host parasites in marine ecosystems. Copyright © 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

  13. Effect of light Sphagnum peat on odour formation in the early stages of biowaste composting.

    Science.gov (United States)

    Kurola, Jukka M; Arnold, Mona; Kontro, Merja H; Talves, Matti; Romantschuk, Martin

    2010-05-01

    In the present study, we investigated the effects of two bulking materials, Sphagnum peat and pine wood chips, on the early stages of biowaste composting in two pilot-scale processes. Emphasis was placed on studying the formation conditions of malodorous compost gases in the initial phases of the processes. The results showed that gas emission leaving an open windrow and a closed drum composting system contained elevated concentrations of fermentative microbial metabolites when acid Sphagnum peat (pH 3.2) was used as a bulking material. Moreover, the gas emission of the peat amended drum composter contained a high concentration of odour (up to 450,000oum(-3) of air). The highest odour values in the outlet gas of peat amended composts coincided with the elevated concentrations of volatile organic compounds such as acetoin and buthanedion. We conclude that the acidifying qualities of composting substrates or bulking material may intensify odour emission from biowaste composts and prolong the early stages of the composting process. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

  14. Effect of NPK fertilization and elemental sulphur on growth and yield of lowbush blueberry

    Directory of Open Access Journals (Sweden)

    M. STARAST

    2008-12-01

    Full Text Available The aim of the research was to determine the effect of fertilizers on the pH of former arable soils and on the growth and the yield of the lowbush blueberry (Vaccinium angustifolium Ait.. Lowbush blueberry fertilization experiments were established in 1999 at two locations – at Kärla, Saaremaa, West Estonia and at Vasula, Tartu County, South Estonia. Experimental sites were situated on different soils: soil pHKCl at Kärla was 5.5 and at Vasula 6.2. Elemental sulphur and acidifying fertilizers (ammonium sulfate, potassium sulfate and superphosphate were used in both plantations. Fertilizers were applied based on nutrient in the soil and sulphur was applied at 100 g m–2. Plant growth was recorded in 2001, 2002 and 2003. A positive influence of NPK fertilization on yield was found in both Kärla and Vasula, and yield were 336 g and 41 g higher compared to the control, respectively. The vegetative growth and yield of blueberry depended significantly on soil pH. Elemental sulphur increased soil acidity and on loamy sand soil did not increase plant productivity. The sulphur effect on soil pH began to decrease three years after application. Sulphur can be recommended to increase soil acidity in nutrient-rich soil but, not nutrient poor soil with light texture, where only NPK fertilizers were effective.;

  15. Effects of 42 deg. C hyperthermia on intracellular pH in ovarian carcinoma cells during acute or chronic exposure to low extracellular pH

    International Nuclear Information System (INIS)

    Wahl, Miriam L.; Bobyock, Suzanne B.; Leeper, Dennis B.; Owen, Charles S.

    1997-01-01

    Purpose: To determine whether intracellular pH (pH i ) is affected during hyperthermia in substrate-attached cells and whether acute extracellular acidification potentiates the cytotoxicity of hyperthermia via an effect on pH i . Methods and Materials: The pH i was determined in cells attached to extracellular matrix proteins loaded with the fluorescent indicator dye BCECF at 37 deg. C and during 42 deg. C hyperthermia at an extracellular pH (pH e ) of 6.7 or 7.3 in cells. Effects on pH i during hyperthermia are compared to effects on clonogenic survival after hyperthermia at pH e 7.3 and 6.7 of cells grown at pH e 7.3, or of cells grown and monitored at pH e 6.7. Results: The results show that pH i values are affected by substrate attachments. Cells attached to extracellular matrix proteins had better signal stability, low dye leakage and evidence of homeostatic regulation of pH i during heating. The net decrease in pH i in cells grown and assayed at pH e = 7.3 during 42 deg. C hyperthermia was 0.28 units and the decrease in low pH adapted cells heated at pH e = 6.7 was 0.14 units. Acute acidification from pH e = 7.3 to pH e = 6.7 at 37 deg. C caused an initial reduction of 0.5-0.8 unit in pH i , but a partial recovery followed during the next 60-90 min. Concurrent 42 deg. C hyperthermia caused the same initial reduction in pH i in acutely acidified cells, but inhibited the partial recovery that occurred during the next 60-90 min at 37 deg. C. After 4 h at 37 deg. C, the net change in pH i in acutely acidified cells was 0.30 pH unit, but at 42 deg. C is 0.63 pH units. The net change in pH i correlated inversely with clonogenic survival. Conclusions: Hyperthermia causes a pH i reduction in cells which was smaller in magnitude by 50% in low pH adapted cells. Hyperthermia inhibited the partial recovery from acute acidification that was observed at 37 deg. C in substrate attached cells, in parallel with a lower subsequent clonogenic survival

  16. Natural acidification changes the timing and rate of succession, alters community structure, and increases homogeneity in marine biofouling communities.

    Science.gov (United States)

    Brown, Norah E M; Milazzo, Marco; Rastrick, Samuel P S; Hall-Spencer, Jason M; Therriault, Thomas W; Harley, Christopher D G

    2018-01-01

    Ocean acidification may have far-reaching consequences for marine community and ecosystem dynamics, but its full impacts remain poorly understood due to the difficulty of manipulating pCO 2 at the ecosystem level to mimic realistic fluctuations that occur on a number of different timescales. It is especially unclear how quickly communities at various stages of development respond to intermediate-scale pCO 2 change and, if high pCO 2 is relieved mid-succession, whether past acidification effects persist, are reversed by alleviation of pCO 2 stress, or are worsened by departures from prior high pCO 2 conditions to which organisms had acclimatized. Here, we used reciprocal transplant experiments along a shallow water volcanic pCO 2 gradient to assess the importance of the timing and duration of high pCO 2 exposure (i.e., discrete events at different stages of successional development vs. continuous exposure) on patterns of colonization and succession in a benthic fouling community. We show that succession at the acidified site was initially delayed (less community change by 8 weeks) but then caught up over the next 4 weeks. These changes in succession led to homogenization of communities maintained in or transplanted to acidified conditions, and altered community structure in ways that reflected both short- and longer-term acidification history. These community shifts are likely a result of interspecific variability in response to increased pCO 2 and changes in species interactions. High pCO 2 altered biofilm development, allowing serpulids to do best at the acidified site by the end of the experiment, although early (pretransplant) negative effects of pCO 2 on recruitment of these worms were still detectable. The ascidians Diplosoma sp. and Botryllus sp. settled later and were more tolerant to acidification. Overall, transient and persistent acidification-driven changes in the biofouling community, via both past and more recent exposure, could have important

  17. Construction of g-C_3N_4/Al_2O_3 hybrids via in-situ acidification and exfoliation with enhanced photocatalytic activity

    International Nuclear Information System (INIS)

    Wang, Xiao-jing; Liu, Chao; Li, Xu-li; Li, Fa-tang; Li, Yu-pei; Zhao, Jun; Liu, Rui-hong

    2017-01-01

    Highlights: • Ultrathin g-C_3N_4/Al_2O_3 hybrids are prepared via in-situ reaction. • The structure modification role of in-situ formed HNO_3 for g-C_3N_4 is found. • The ultrathin g-C_3N_4 nanosheets are formed by the acidified melamine and Al(OH)_3. • In-situ calcination of melamine and Al(OH)_3 benefits the contact of C_3N_4 and Al_2O_3. • The activity of g-C_3N_4/Al_2O_3 is 16.6 times that of pristine g-C_3N_4 in degrading RhB. - Abstract: Homogeneous ultrathin g-C_3N_4 nanosheets/Al_2O_3 heterojunctions are synthesized using melamine and Al(NO_3)_3 via in-situ reaction and the following thermal polymerization approach. The in-situ reaction between melamine and Al(NO_3)_3 results in the existence of HNO_3-acidified melamine and Al(OH)_3 aggregates via the hydrolysis of Al(NO_3)_3. After thermal polymerization, the aggregates are converted to g-C_3N_4/Al_2O_3 composites. The thermal polymerization of acidified melamine and the support effect of aluminum hydroxide for g-C_3N_4 during the calcination process lead to highly dispersed amrophous Al_2O_3 on ultrathin g-C_3N_4 nanosheets, which is beneficial for the separation of photogenerated electron-hole pairs in the heterojunction. The degradation rate for Rhodamine B (RhB) over the most activie sample is 16.6 times than that of pristine g-C_3N_4 under visible light irradiation, which can be attributed to the high specific surface area, highly dispersion of amorphous Al_2O_3 on ultrathin g-C_3N_4 nanosheet, and the effective electrons transfer from g-C_3N_4 to the amorphous Al_2O_3.

  18. The Hanle effect and level-crossing spectroscopy

    CERN Document Server

    Strumia, Franco

    1991-01-01

    I am most pleased and, in a way, I feel honored to write the Foreword for the book The Hanle Effect and Level-Crossing Spectroscopy, which covers such a very wide range of applications not only in the initial areas of atomic and molecular physics, but also in solid state physics, solar physics, laser physics, and gravitational metrology. To link these fields together in a coherent way has been the merit of the editors of the book, who attracted most distinguished authors for writing the chapters. In retrospect to Hanle's discovery of quantum mechanical coherence between two quantum states about 65 years ago, this book demonstrates the enormous impact and central importance the effect has had, and most vividly still has, on modern physics. On the other hand, the concept of quantum mechanical coherence, which is an outgrowth of the linear super­ position principle of quantum states, has been evident through a consider­ able number of experimental methods beyond the original Hanle effect; some of these methods...

  19. l'india di mario luzi: «questa enorme, meravigliosa forza del paradosso

    African Journals Online (AJOL)

    User

    frastagliato in una straordinaria varietà di quesiti? Il paese sembra rianimato da un risveglio ancora recente, un miliardo di uomini sente di costituire, se non altro, l'argomento della politica nazionale. […] Dietro la facciate delle istituzioni che vorrebbe essere trasparente s'indovina un dibattimento se non una lotta in corso.

  20. A Three-Dimensional Enormous Surface Area Aluminum Microneedle Array with Nanoporous Structure

    OpenAIRE

    Chen, Po Chun; Hsieh, Sheng Jen; Chen, Chien Chon; Zou, Jun

    2013-01-01

    We proposed fabricating an aluminum microneedle array with a nanochannel structure on the surface by combining micromachining, electrolyte polishing, and anodization methods. The microneedle array provides a three-dimensional (3D) structure that possesses several hundred times more surface area than a traditional nanochannel template. Therefore, the microneedle array can potentially be used in many technology applications. This 3D microneedle array device can not only be used for painless inj...

  1. A Three-Dimensional Enormous Surface Area Aluminum Microneedle Array with Nanoporous Structure

    Directory of Open Access Journals (Sweden)

    Po Chun Chen

    2013-01-01

    Full Text Available We proposed fabricating an aluminum microneedle array with a nanochannel structure on the surface by combining micromachining, electrolyte polishing, and anodization methods. The microneedle array provides a three-dimensional (3D structure that possesses several hundred times more surface area than a traditional nanochannel template. Therefore, the microneedle array can potentially be used in many technology applications. This 3D microneedle array device can not only be used for painless injection or extraction, but also for storage, highly sensitive detection, drug delivery, and microelectrodes. From the calculation we made, the microneedle array not only increases surface area, but also enlarges the capacity of the device. Therefore, the microneedle array can further be used on many detecting, storing, or drug delivering applications.

  2. A Three-Dimensional Enormous Surface Area Aluminum Microneedle Array with Nanoporous Structure

    International Nuclear Information System (INIS)

    Chen, P.Ch.; Zou, J.; Hsieh, Sh.J.; Chen, Ch.Ch.

    2013-01-01

    We proposed fabricating an aluminum micro needle array with a nano channel structure on the surface by combining micromachining, electrolyte polishing, and anodization methods. The micro needle array provides a three-dimensional (3D) structure that possesses several hundred times more surface area than a traditional nano channel template. Therefore, the micro needle array can potentially be used in many technology applications. This 3D micro needle array device can not only be used for painless injection or extraction, but also for storage, highly sensitive detection, drug delivery, and microelectrodes. From the calculation we made, the micro needle array not only increases surface area, but also enlarges the capacity of the device. Therefore, the micro needle array can further be used on many detecting, storing, or drug delivering applications.

  3. Mathematics and the Internet: A Source of Enormous Confusion and Great Potential

    Science.gov (United States)

    2009-05-01

    the mathematics and physics commu- nities to parallel developments in the Internet research arena, where the various non-rigorous and rigorous results...based Internet research in general, and Internet-specific validation efforts in particular, have to say about the popular scale-free modeling paradigm...ironically, the story starts with a highly-cited paper in the Internet research arena by Faloutsos et al. [27]. Relying on available measure- ments and

  4. Parts giants will bring enormous change to China’s automotive aftersales market

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    <正> The fourth worldwide largest independent automotive parts supplier-DENSO-is beefing up its presence in China by means of regional agents or chain stores, establishing its marketing system and developing the aftersales market in China. Some insiders have noticed that, recently, harvests at the engine OEM market, these giants have also flocked into the aftersales market.

  5. Self-organized TiO2 nanotubular arrays for photoelectrochemical hydrogen generation: effect of crystallization and defect structures

    International Nuclear Information System (INIS)

    Mahajan, V K; Misra, M; Raja, K S; Mohapatra, S K

    2008-01-01

    The effect of crystallization and surface chemistry of nanotubular titanium dioxide (TiO 2 ) in connection with the photoelectrochemical process is reported in this investigation. TiO 2 nanotubular arrays were synthesized by a simple anodization process in an acidified fluoride electrolyte at room temperature. The TiO 2 nanotubes were amorphous in as-anodized condition; their transformation to crystalline phases was a function of annealing temperature and gaseous environment. The anatase phase was observed predominantly after annealing in non-oxidizing atmospheres, whereas annealing in an oxygen environment showed a mixture of anatase and rutile phases. X-ray photoelectron spectroscopy was used to determine the chemical environment of the surface, which revealed the presence of phosphate, oxygen vacancies and pentacoordinated Ti in hydrogen annealed samples. Diffuse reflectance photospectrometry of non-oxygen annealed samples showed long absorption tails extending in the visible region. The photoelectrochemical response of the TiO 2 nanotubes annealed in different conditions was investigated. Photoelectrochemical performance under simulated solar light was improved by annealing the nanotubular TiO 2 samples in non-oxidizing environment

  6. Thermal legacies: transgenerational effects of temperature on growth in a vertebrate.

    Science.gov (United States)

    Salinas, Santiago; Munch, Stephan B

    2012-02-01

    Transgenerational plasticity (TGP), a generalisation of more widely studied maternal effects, occurs whenever environmental cues experienced by either parent prior to fertilisation results in a modification of offspring reaction norms. Such effects have been observed in many traits across many species. Despite enormous potential importance-particularly in an era of rapid climate change-TGP in thermal growth physiology has never been demonstrated for vertebrates. We provide the first evidence for thermal TGP in a vertebrate: given sufficient time, sheepshead minnows adaptively program their offspring for maximal growth at the present temperature. The change in growth over a single generation (c. 30%) exceeds the single-generation rate of adaptive evolution by an order of magnitude. If widespread, transgenerational effects on thermal performance may have important implications on physiology, ecology and contemporary evolution, and may significantly alter the extinction risk posed by changing climate. © 2011 Blackwell Publishing Ltd/CNRS.

  7. Biology and chemistry of three Pennsylvania lakes: responses to acid precipitation

    Energy Technology Data Exchange (ETDEWEB)

    Bradt, P.T.; Dudley, J.L.; Berg, M.B.; Barrasso, D.S.

    1986-09-01

    The biology and chemistry of three northeastern Pennsylvania lakes was studied from summer 1981 through summer 1983 to evaluate lakes with different sensitivities to acidification. At the acidified lake there were fewer phytoplankton and zooplankton species than at the moderately sensitive lakes. The most numerous plankton species in all three lakes are reportedly acid tolerant. Among the benthic macro-invertebrates (BMI) there were more acid tolerant Chironomidae at the acidified lake, but more acid intolerant Ephemeroptera and Mollusca and a higher wet weight at the least sensitive lake. There were no differences among the lakes' BMI mean total numbers or mean number of taxa. The fish community at the acidified lake was dominated by stunted Lepomis gibbosus, but L. machrochirous were most abundant in the other lakes. Principal component analysis suggested a shift in all three lakes over the sampling period toward combined lower pH, alkalinity, specific conductance, Ca and Mg and higher Al and Mn. Such chemical changes have been associated with acidification. The rate and extent of acidification appeared to be controlled by geological and hydrological characteristics of the drainage basins. 38 refs.

  8. Biology and chemistry of tree Pennsylvania lakes: responses to acid precipitation. [Lepomis gibbosus; Lepomis machrochirous

    Energy Technology Data Exchange (ETDEWEB)

    Bradt, P.T.; Dudley, J.L.; Berg, M.B.; Barrasso, D.S.

    1986-01-01

    The biology and chemistry of three northeastern Pennsylvania lakes was studied from summer 1981 through summer 1983 to evaluate lakes with different sensitivities to acidification. At the acidifies lake (total alkalinity par. delta 0.0 ..mu..eq L/sup -1/) there were fewer phytoplankton and zooplankton species than at the moderately sensitive lakes. The most numerous plankton species in all three lakes are reportedly acid tolerant. Among the benthic macro- invertebrates (BMI) there were more acid tolerant Chironomidae at the acidified lake, but more acid intolerant Ephemeroptera and Mollusca and a higher wet weight at the least sensitive lake. There were no differences among the lakes' BMI mean total numbers or mean number of taxa. The fish community at the acidified lake was dominated by stunted Lepomis gibbosus, but L. machrochirous were most abundant in the other lakes. Principal component analysis suggested a shift in all three lakes over the sampling period toward combined lower pH, alkalinity, specific conductance, Ca, and Mg and higher Al and Mn. Such chemical changes have been associated with acidification. The rate and extent of acidification appeared to be controlled by geological and hydrological characteristics of the drainage basins.

  9. Whole-lake algal responses to a century of acidic industrial deposition on the Canadian Shield

    International Nuclear Information System (INIS)

    Vinebrooke, R.D.; Dixit, S.S.; Graham, M.D.; Gunn, J.M.; Chen, Y.-W.; Belzile, N.

    2002-01-01

    A century of cultural acidification is hypothesized to have altered algal community structure in boreal lakes. To date, this hypothesis has remained untested because of both the lack of data predating the onset of industrial pollution and incomplete estimates of whole-lake algal community structure. High-pressure liquid chromatography (HPLC) of sedimentary pigments was used to quantify whole-lake algal responses to acid deposition in six boreal lakes located in Killarney Park, Ontario, Canada. Concomitant significant increases in chlorophyll and carotenoid concentrations, diatom-inferred lake acidity, and metal levels since 1900 suggested that algal abundances in four acidified lakes and one small, circumneutral lake were enhanced by aerial pollution. An alternate explanation is that increased acidity and underwater light availability in the acidified lakes shifted algal abundance towards phytobenthos and deepwater phytoplankton, whose pigment signatures were better preserved in the sediments. Taxonomically diagnostic pigment stratigraphies were consistent with shifts in algal community structure towards filamentous green phytobenthos and deepwater phytoflagellates in the acidified lakes. Our findings suggest that decades of aerial pollution have altered the base of foodwebs in boreal lakes, potentially rendering them less resilient to other environmental stressors. (author)

  10. Analysis of tree samples from an acidic and a limed environment by means of PIXE

    International Nuclear Information System (INIS)

    Pernestaal, K.; Li, Hong-Ko; Jonsson, B.; Haellgren, J.-E.

    1991-01-01

    Three tree core samples of Norway spruce (Picea Abies) have been analysed by PIXE. The concentration profiles for 17 elements heavier than sodium have been determined by means of one PIXE measurement every 2 mm along the samples. The samples were collected at Faexboda, an experimental area north of Uppsala where artificially acidified and limed environmental experiments are made. One sample from each of an acidified, a limed and a reference area has been collected. The main interest is focused on potassium, calcium, manganese, iron, copper and zinc. All three samples have about the same concentration profile in the heartwood. Large differences are observed in the sapwood. In the sample from the limed area the calcium content is much higher and the iron and chromium contents are lower than in the sample from the reference area. In the sample from the acidified area the calcium and potassium contents are lower and the manganese, iron and chromium contents are much higher than in the sample from the reference area. The observed concentration profiles of trace elements in trees indicate that changes in the environment due to acidification can be quantified at an early stage by means of PIXE. (author)

  11. Pathological effects of acetone cyanohydrin in swiss rats

    Directory of Open Access Journals (Sweden)

    Marcos Natal Rufino

    Full Text Available ABSTRACT Cassava has been widely used for animal and human nutrition. It has also been demonstrated to have antineoplastic and anthelmintic properties. Toxicity due to cassava consumption has been reported in ruminants and laboratory animals; therefore, this study aimed to investigate the toxic effects of acetone cyanohydrin, a metabolite of linamarin that is present in cassava, in Wistar rats. Six groups of five animals each were used to evaluate the toxic effects of acetone cyanohydrin administered at 25 (G1, 50 (G2, 75 (G3, 100 (G4 and 125 (G5 µmol/kg as a single oral dose. The control group received acidified water (pH 3.5. The animals were monitored after administration of acetone cyanohydrin, and clinical symptoms were recorded. Serum enzyme levels were measured to assess the kidney and liver function. During necropsy, tissue samples were collected for histopathological examination. After administration, some animals in the G2, G4, and G5 groups presented neurological symptoms such as convulsions, involuntary muscle contraction, staggering gait, motor coordination disability, prostration, and mydriasis. All of the animals in the G5 and four animals in the G4 group died seven minutes after the administration of acetone cyanohydrin. Animals in the other groups, particularly in G2, recovered from the acute phase. Biochemical analysis revealed hepatic lesions and liver dysfunction. Histopathology revealed severe lesions in both the liver and brain. In conclusion, acetone cyanohydrin has toxic effects in the liver, lung, and central nervous system in rats; however, at concentrations up to 25 µmol/kg, the animals could survive the acute phase.

  12. Clinical trial: the effects of a fermented milk containing three probiotic bacteria in patients with irritable bowel syndrome - a randomized, double-blind, controlled study.

    Science.gov (United States)

    Simrén, M; Ohman, L; Olsson, J; Svensson, U; Ohlson, K; Posserud, I; Strid, H

    2010-01-15

    The effects of probiotic bacteria in IBS remain controversial. To study the effects of a probiotic product on IBS symptoms. We randomized 74 IBS patients to receive 8 weeks of daily treatment with 400 mL milk fermented with the yoghurt bacteria and containing Lactobacillus paracasei, ssp. paracasei F19, Lactobacillus acidophilus La5 and Bifidobacterium lactis Bb12 (Cultura; active) or acidified milk without these bacteria (control). The primary endpoint was the proportion of subjects reporting adequate relief of their IBS symptoms at least 50% of the weeks. IBS symptom severity, psychological symptoms and quality of life were assessed. The proportion of responders was 38% (14/37 patients) in the active group and 27% (10/37 patients) in the control group (P = 0.3). IBS symptom severity improved significantly in both groups during the treatment period. This change was greater in the active group during the first 2 weeks, but thereafter, no significant group differences were seen. We could not detect a clearly positive effect of fermented milk containing three probiotic bacteria on GI symptoms in IBS patients compared with the control treatment. However, a trend towards a more favourable effect during the first weeks was seen in the active group.

  13. Unconventional resource's production under desorption-induced effects

    Directory of Open Access Journals (Sweden)

    S. Sina Hosseini Boosari

    2016-06-01

    We have developed a numerical model to study the effect of changes in porosity, permeability and compaction on four major U.S. shale formations considering their Langmuir isotherm desorption behavior. These resources include; Marcellus, New Albany, Barnett and Haynesville Shales. First, we introduced a model that is a physical transport of single-phase gas flow in shale porous rock. Later, the governing equations are implemented into a one-dimensional numerical model and solved using a fully implicit solution method. It is found that the natural gas production is substantially affected by desorption-induced porosity/permeability changes and geomechancis. This paper provides valuable insights into accurate modeling of unconventional reservoirs that is more significant when an even small correction to the future production prediction can enormously contribute to the U.S. economy.

  14. USED IN THE MANUFACTURE OF EMULSION RUBBER WASTE OF SUGAR MANUFACTURE – MOLASSES

    OpenAIRE

    S. S. Nikulin; .; N. S. Nikulina

    2015-01-01

    Currently, much attention is paid to development, allowing use of waste and by-products formed during certain manufacturing processes. This allows you to either reduce or completely eliminate the use of valuable and expensive raw materials. The use of this raw material in the production of synthetic rubber will not only reduce the cost of coagulation and acidifying agents, but also to improve the environmental situation. Featured in some cases effective coalescing agents based on polymeric qu...

  15. [Medical and socio-economic effects of early rehabilitation of patients with acute cerebral vascular accidents].

    Science.gov (United States)

    Popov, A V; Cherednichenko, T V; Lishnevskiĭ, S A; Khomenko, T V; Prigornitskaia, Ia I

    2012-01-01

    Acute ischemic brain injury (stroke, stroke), a leader among the causes of morbidity and mortality in the world. This pathology is one of the most pressing health and social problems that cause enormous economic damage to society, due to the high fatality rate, significant disability and social maladjustment of patients, which is based in most cases are the motor and cognitive impairment. Despite the fact that, currently established risk factors and pathophysiological basis of this disease, the availability of effective methods of diagnosing illness, still a practicing neurologist in some cases difficult to find adequate therapy that could effectively reach a well-established neurological deficit. Therefore the search for treatments that effectively reduce the health and social consequences of vascular damage to the brain, is one of the priority areas of neurology.

  16. Synergetic enhancement effect of ionic liquid and diethyldithiocarbamate on the chemical vapor generation of nickel for its atomic fluorescence spectrometric determination in biological samples

    International Nuclear Information System (INIS)

    Zhang Chuan; Li Yan; Wu Peng; Yan Xiuping

    2009-01-01

    Room-temperature ionic liquid in combination with sodium diethyldithiocarbamate (DDTC) was used to synergetically improve the chemical vapor generation (CVG) of nickel. Volatile species of nickel were effectively generated through reduction of acidified analyte solution with KBH 4 in the presence of 0.02% DDTC and 25 mmol L -1 1-butyl-3-methylimidazolium bromide ([C 4 mim]Br) at room temperature. Thus, a new flow injection (FI)-CVG-atomic fluorescence spectrometric (FI-CVG-AFS) method was developed for determination of nickel with a detection limit of 0.65 μg L -1 (3 s) and a sampling frequency of 180 h -1 . With consumption of 0.5 mL sample solution, an enhancement factor of 2400 was obtained. The precision (RSD) for eleven replicate determinations of 20 μg L -1 Ni was 3.4%. The developed FI-CVG-AFS method was successfully applied to determination of trace Ni in several certified biological reference materials.

  17. EFFECTS OF PERCEIVED VALUE AND ITS DIMENSIONS ON WORD OF MOUTH ADVERTISING AMONG CUSTOMERS OF SPORTS CLUBS OF THE CITY OF SANANDAJ, IRAN

    OpenAIRE

    Forut Amjadi; Mozafar Yektayar; Mojgan Khodamorad Pour

    2016-01-01

    The present study investigates the effects of perceived value on word of mouth advertising among customers of sports clubs of the city of Sanandaj. The applied research method is descriptive-correlative and the population of research is consisted of the entire customers of sports clubs of Sanandaj. As a result of enormousness of the population, through the Cochran’s formula, a sample size was determined and samples were respectively selected through a simple random sampling. In addition, data...

  18. Yields of cotton and other crops as affected by applications of sulfuric acid in irrigation water

    Energy Technology Data Exchange (ETDEWEB)

    Christensen, P.D.; Lyerly, P.J.

    1954-01-01

    Effects of sulfuric acid on crop yields and on some physical and chemical properties of a calcareous soil were investigated in a field experiment from 1947 through 1952. On cotton plots, the treatments consisted of applications of irrigation water containing no acid (pH 8.3), water acidified to pH 6, and water acidified to pH 2.3. Cotton was grown five seasons followed by sesbania the sixth season. A test on alfalfa was established using irrigation water not acidified and water acidifeid to pH 4. Alfalfa was grown for 3 years. The fourth year the alfalfa was plowed under and a crop of corn was raised. Cotton yields on the acid plots relative to the checks became progressively higher (with two exceptions) from one year to the next; however, in only one year (1950) were differences in yield statistically significant. With sesbania following cotton, highly significant yield increases resulted from the high acid treatment. Alfalfa yields on the acid plots became progressively greater relative to the non-acid plots, but yield differences were not significant. In cotton leaves, the acid treatments resulted in increased uptake of magnesium, sulfur, and phosphorus, but the increases were probably not significant. Uptake of sodium, potassium, calcium, manganese, and iron were not appreciably affected. In sesbania, the acid treatments did not significantly alter the uptake of any of the plant nutrients determined. There was some indication, however, that the uptake of sodium and iron was reduced by the acidification. The results of this study support the view that soil acidification on calcareous soils may improve the soil physical conditions and result in increased yields, particularly in some crops. The application of acid in the irrigation water did not prove to be economically feasible. 12 references, 1 figure, 7 tables.

  19. The effect of advertising on children and adolescents

    NARCIS (Netherlands)

    Lapierre, M.A.; Fleming-Milici, F.; Rozendaal, E.; McAlister, A.R.; Castonguay, J.

    2017-01-01

    In ~100 years, marketing to children went from a severely frowned upon practice to an integral part of growing up as companies came to realize that investing in marketing to children and adolescents provides excellent immediate and future dividends. Each year, enormous sums of money are spent to

  20. Dual field effects in electrolyte-gated spinel ferrite: electrostatic carrier doping and redox reactions.

    Science.gov (United States)

    Ichimura, Takashi; Fujiwara, Kohei; Tanaka, Hidekazu

    2014-07-24

    Controlling the electronic properties of functional oxide materials via external electric fields has attracted increasing attention as a key technology for next-generation electronics. For transition-metal oxides with metallic carrier densities, the electric-field effect with ionic liquid electrolytes has been widely used because of the enormous carrier doping capabilities. The gate-induced redox reactions revealed by recent investigations have, however, highlighted the complex nature of the electric-field effect. Here, we use the gate-induced conductance modulation of spinel ZnxFe₃₋xO₄ to demonstrate the dual contributions of volatile and non-volatile field effects arising from electronic carrier doping and redox reactions. These two contributions are found to change in opposite senses depending on the Zn content x; virtual electronic and chemical field effects are observed at appropriate Zn compositions. The tuning of field-effect characteristics via composition engineering should be extremely useful for fabricating high-performance oxide field-effect devices.

  1. Use of soil-streamwater relationships to assess regional patterns of acidic deposition effects in the northeastern USA

    Science.gov (United States)

    Siemion, Jason; Lawrence, Gregory B.; Murdoch, Peter S.

    2013-01-01

    Declines of acidic deposition levels by as much as 50% since 1990 have led to partial recovery of surface waters in the northeastern USA but continued depletion of soil calcium through this same period suggests a disconnection between soil and surface water chemistry. To investigate the role of soil-surface water interactions in recovery from acidification, the first regional survey to directly relate soil chemistry to stream chemistry during high flow was implemented in a 4144-km2 area of the Catskill region of New York, where acidic deposition levels are among the highest in the East.More than 40% of 95 streams sampled in the southern Catskill Mountains were determined to be acidified and had inorganic monomeric aluminum concentrations that exceeded a threshold that is toxic to aquatic biota. More than 80% likely exceeded this threshold during the highest flows, but less than 10% of more than 100 streams sampled were acidified in the northwestern portion of the region. Median Oa horizon soil base saturation ranged from 50% to 80% at 200 sites across the region, but median base saturation in the upper 10 cm of the B horizon was less than 20% across the region and was only 2% in the southern area. Aluminum is likely to be interfering with root uptake of calcium in the mineral horizon in approximately half the sampled watersheds. Stream chemistry was highly variable over the Catskill region and, therefore, did not always reflect the calcium depletion of the B horizon that our sampling suggested was nearly ubiquitous throughout the region. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.

  2. Femoral Cortical Bone Mineral Density and Biomechanical Properties in Sheep Consuming an Acidifying Diet

    Directory of Open Access Journals (Sweden)

    Eileen S. Hackett

    2009-01-01

    Full Text Available Dietary acidity is a likely contributor to the development of osteoporosis. Dietary acidosis in an ovine model has effects on trabecular bone that have been previously shown to mimic human osteoporosis. Effects on cortical bone using this model have not been investigated. The objective of this study was to examine the effects of dietary acidosis on cortical bone mineral density and material properties. Skeletally mature ovariectomized (OVX sheep consumed either a normal diet (ND or a metabolic acidosis diet (MA for 6 or 12 months. Whole femoral and cortical bone beam BMD was determined using dual energy x-ray absorptiometry (DEXA. Beams were then subjected to three point flexure monotonically to failure to determine strength and modulus and then ashed to determine percent mineralization. Femoral BMD in adult OVX ND 6 mo sheep was significantly greater than those in the non-OVX ND group. The BMD in the MA groups was lower than the control non-OVX ND group. Cortical beams had significantly decreased modulus in all MA and OVX groups when compared with the non-OVX ND group and a tendency towards decreased strength in all groups with significance only in the OVX ND 6 mo sheep. Percent mineralization increased in MA and OVX groups when compared to the non-OVX ND group and was significantly increased in the OVX ND 6 mo and OVX MA 12 mo groups. A significant correlation was seen between BMD of the beam and breaking strength and modulus. Dietary acidity impacts cortical bone and results in reduced material properties that may contribute to failure.

  3. Working group on psychological effects of nuclear accidents, Kiev, USSR, 28 May - 1 June 1990

    International Nuclear Information System (INIS)

    1990-01-01

    The population in the areas affected by the Chernobyl accident is experiencing serious worries about possible health effects. The level of concern appears to be increasing, and the psychological context of the accident should receive detailed consideration. Surveys conducted by Soviet researchers have shown that anxiety has spread for beyond the population of the more heavily contaminated areas. People's awareness of their health status has increased enormously since the accident, and they now attribute many subjective and objective symptoms to radiation exposure. Medical personnel and lay people should be trained in counselling, to enable them to allay the fears people have

  4. 21 CFR 173.325 - Acidified sodium chlorite solutions.

    Science.gov (United States)

    2010-04-01

    ... processing of red meat, red meat parts, and organs as a component of a spray or in the processing of red meat... rinse prior to consumption. (2) The additive is used as a single application in processing facilities as... (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) SECONDARY DIRECT FOOD ADDITIVES PERMITTED IN FOOD FOR...

  5. Nutrient cycling in a strongly acidified mesotrophic lake

    Czech Academy of Sciences Publication Activity Database

    Kopáček, Jiří; Brzáková, Martina; Hejzlar, Josef; Nedoma, Jiří; Porcal, Petr; Vrba, Jaroslav

    2004-01-01

    Roč. 49, č. 4 (2004), s. 1202-1213 ISSN 0024-3590 R&D Projects: GA ČR(CZ) GA206/00/0063; GA ČR(CZ) GA206/03/1583 Institutional research plan: CEZ:AV0Z6017912 Keywords : acidification * nutrients * water chemistry Subject RIV: DA - Hydrology ; Limnology Impact factor: 3.024, year: 2004

  6. How fast is the Patagonian shelf-break acidifying?

    Science.gov (United States)

    Orselli, Iole B. M.; Kerr, Rodrigo; Ito, Rosane G.; Tavano, Virginia M.; Mendes, Carlos Rafael B.; Garcia, Carlos A. E.

    2018-02-01

    Anthropogenic carbon (Cant) concentration is determined according to the TrOCA method, from carbonate system data and hydrographic parameters collected during two consecutive spring cruises (2007 and 2008) in the Argentinean Patagonian shelf-break zone between 36°S and 50°S. Cant has intruded the water column until intermediate depths, with no Cant below 1000 m, in the deeper waters (i.e., North Atlantic Deep Water and Antarctic Bottom Water) of the Northern sector of the study area (i.e., North of 38°S). The higher Cant concentration is observed in Subantarctic Shelf Water in the Southern region, whereas in the Northern sector both Tropical Water and South Atlantic Central Water are equally affected by Cant intrusion. The Antarctic Intermediate Water represents the depth-limit achieved by Cant penetration, reinforcing the role that this water mass plays as an important vehicle to transport Cant to the oceans interior. The estimated Cant average (± method precision) is 46.6 ± 5.3 μmol kg- 1, considering the full depth of the water column. The ocean acidification state (ΔpH) shows an average (± standard deviation) of - 0.11 ± 0.05, thus, indicating an annual pH reduction of - 0.0010 yr- 1 since the Industrial Revolution (c.a. 1750). The degree of aragonite saturation is lowered towards undersaturation levels of calcite. The Patagonian shelf and shelf-break zones-a strong CO2 sink region in the global ocean-are likely a key area for Cant intrusion in the southwestern South Atlantic Ocean.

  7. Model study on acidifying wet deposition in East Asia during wintertime

    Science.gov (United States)

    Han, Zhiwei; Ueda, Hiromasa; Sakurai, Tatsuya

    A regional air quality model (RAQM) has been developed and applied together with an aerosol model to investigate the states and characteristics of wet deposition in East Asia in December 2001. Model simulation is performed with monthly based emission inventory [Streets, D.G., Bond, T.C., Carmichael, G.R., Fernandes, S.D., Fu, Q., He, D., Klimont, Z., Nelson, S. M., Tsai, N.Y., Wang, M.Q., Woo, J.-H., Yarber, K.F., 2003. An inventory of gaseous and primary emissions in Asia in the year 2000. Journal of Geophysical Research 108(D21), 8809] and meteorological fields derived from MM5. Model results are compared with extensive monitoring data including relevant gaseous species and ions in precipitation. The validation demonstrates that this model system is able to represent most of the major physical and chemical processes involved in acid deposition and reproduces concentrations reasonably well, within a factor of 2 of observations in general. The study shows that the regions with pH less than 4.5 are mainly located in southwestern China, parts of the Yangtze Delta, the Yellow Sea and the Korean peninsula, indicating wide regions of acid precipitation in East Asia in wintertime. Japan islands mainly exhibit pH values of 4.5-5.0, whereas over wide areas of northern China, pH values are relatively high (⩾5.0) due to neutralization by alkaline materials such as calcium-laden particles and ammonia, which are more abundant in northern China than that in southern China. While acid rain over most of China is still characterized by sulfur-induced type, considerable areas of eastern China and the western Pacific Rim are found to be more affected by nitric acid than sulfuric acid in acidification of precipitation, which is supposed to result from a combined effect of variations in photochemistry and emission, suggesting the increasing importance of NO x emission in these regions.

  8. Effect of Giant Rat's Tail Grass (Sporobolus pyramidalis p.beauv) on ...

    African Journals Online (AJOL)

    ADOWIE PERE

    Hydrocarbon (TPH) and Heavy Metals content of Crude Oil Polluted Soils”. *. 1. CHUKA ... heavy metals (chromium, Cr and lead, Pb) were quantified as an index for assessing the ... water, causes enormous economic loss and ecological.

  9. New insights into the sorption mechanism of cadmium on red mud

    International Nuclear Information System (INIS)

    Luo Lei; Ma Chenyan; Ma Yibing; Zhang Shuzhen; Lv Jitao; Cui Mingqi

    2011-01-01

    Effectiveness and mechanism of cadmium (Cd) sorption on original, acidified and ball milling nano-particle red muds were investigated using batch sorption experiments, sequential extraction analysis and X-ray absorption near edge structure (XANES) spectroscopy. The maximum sorption capacity of Cd was 0.16, 0.19, and 0.21 mol/kg for the original, acidified, and nano-particle red muds at pH 6.5, respectively. Both acidification and ball-milling treatments significantly enhanced Cd sorption and facilitated transformation of Cd into less extractable fractions. The Cd L III -edge XANES analysis indicated the formation of inner-sphere complexes of Cd similar to XCdOH (X represents surface groups on red mud) on the red mud surfaces although outer-sphere complexes of Cd were the primary species. This work shed light on the potential application of red mud to remediate Cd-contaminated soils and illustrated the promising tool of XANES spectroscopy for speciation of multicomponent systems of environmental relevance. - Graphical abstract: Display Omitted Highlights: → Red mud has a strong affinity for Cd contaminants. → Ball-milling treatments significantly enhance Cd sorption on red mud. → Cadmium partially formed inner-sphere complexes on the red mud surfaces. → Red mud can be used to remediate Cd contaminated soils effectively. - Cadmium can be strongly sorbed and partially forms inner-sphere complexes on red mud.

  10. Managing ecosystems without prior knowledge: pathological outcomes of lake liming

    Directory of Open Access Journals (Sweden)

    David G. Angeler

    2017-12-01

    Full Text Available Management actions often need to be taken in the absence of ecological information to mitigate the impact of pressing environmental problems. Managers counteracted the detrimental effects of cultural acidification on aquatic ecosystems during the industrial era using liming to salvage biodiversity and ecosystem services. However, historical contingencies, i.e., whether lakes were naturally acidic or degraded because of acidification, were largely unknown and therefore not accounted for in management. It is uncertain whether liming outcomes had a potentially detrimental effect on naturally acidic lakes. Evidence from paleolimnological reconstructions allowed us to analyze community structure in limed acidified and naturally acidic lakes, and acidified and circumneutral references. We analyzed community structure of phytoplankton, zooplankton, macroinvertebrates (littoral, sublittoral, profundal, and fish between 2000 and 2004. Naturally acidic limed lakes formed communities that were not representative of the other lake types. The occurrence of fish species relevant for ecosystem service provisioning (fisheries potential in naturally acidic limed lakes were confounded by biogeographical factors. In addition, sustained changes in water quality were conducive to harmful algal blooms. This highlights a pathological outcome of liming lakes when their naturally acidic conditions are not accounted for. Because liming is an important social-ecological system, sustained ecological change of lakes might incur undesired costs for societies in the long term.

  11. Air-pollution emission control in China: impacts on soil acidification recovery and constraints due to drought.

    Science.gov (United States)

    Duan, Lei; Liu, Jing; Xin, Yan; Larssen, Thorjørn

    2013-10-01

    The Chinese government has established compulsory targets to reduce sulfur dioxide (SO2) and nitrogen oxide (NOx) emissions by 8% and 10%, respectively, during 2010-2015. In this study, the effect of the policy was evaluated by predicting the recovery of acidified forest soil in Chongqing, an area severely impacted by acid rain in southwest China. Since precipitation has decreased significantly in this area in recent years, the impact of drought on soil acidification was also considered. A dynamic acidification model, MAGIC, was used to predict future trends in soil chemistry under different scenarios for deposition reduction as well as drought. We found that the current regulation of SO2 emission abatement did not significantly increase soil water pH values, the Ca2+ to Al3+ molar ratio (Ca/Al), or soil base saturation to the level of 2000 before 2050. NOx emission control would have less of an effect on acidification recovery, while emission reduction of particulate matter could offset the benefits of SO2 reduction by greatly decreasing the deposition of base cations, particularly Ca(2+). Continuous droughts in the future might also delay acidification recovery. Therefore, more stringent SO2 emission control should be implemented to facilitate the recovery of seriously acidified areas in China. © 2013 Elsevier B.V. All rights reserved.

  12. [Multiple sclerosis: socioeconomic effects and impact on quality of life].

    Science.gov (United States)

    Ayuso, Guillermo Izquierdo

    2014-12-01

    Multiple sclerosis (MS) is a disease of the central nervous system (CNS) that affects young adults. Survival is long, more than 35 years, and consequently the disease has a huge socioeconomic impact. The present article discusses the enormous difficulties of carrying out economic assessments in this field but also describes the advances made in research on this topic and the advantages of performing socioeconomic evaluations with increasingly sophisticated tools. We also discuss the need to quantify indirect and intangible costs to translate them into quality of life and subsequently into economic cost, expressed in euros in the case of Spain. The available data indicate that the enormous cost of the disease (1200 million euros per year) is due more to disability-related expenditure than to treatment, which-although expensive-does not represent more than 16-18% of the total expenditure (approximately 200 million euros per year). The increase represented by the cost of MS is not based on higher treatment expenditure but on an increase in the incidence and-especially-the prevalence of the disease. Above all, in the last few years, there has been a considerable rise in the percentage of patients with an indication for treatment. Reflection is therefore needed on the use of drug therapy in MS, since a saving in the most effective products seems to increase the overall cost of MS, while expenditure on these drugs represents a saving in the long-term. Copyright © 2014 Elsevier España, S.L.U. All rights reserved.

  13. Effects of pose and image resolution on automatic face recognition

    NARCIS (Netherlands)

    Mahmood, Zahid; Ali, Tauseef; Khan, Samee U.

    The popularity of face recognition systems have increased due to their use in widespread applications. Driven by the enormous number of potential application domains, several algorithms have been proposed for face recognition. Face pose and image resolutions are among the two important factors that

  14. The chemical and biological response of two remote mountain lakes in the Southern Central Alps (Italy) to twenty years of changing physical and chemical climate

    OpenAIRE

    Andrea LAMI; Pierluigi CAMMARANO; Michele ARMIRAGLIO; Pierisa PANZANI; Roberta BETTINETTI; Alessandra PUGNETTI; Anna M. NOCENTINI; Gabriele A. TARTARI; Simona MUSAZZI; Giuseppe MORABITO; Angela BOGGERO; Marina MANCA; Michela ROGORA; Rosario MOSELLO; Aldo MARCHETTO

    2004-01-01

    Two small high mountain lakes in the Alps were monitored in 1984-2003 to follow their response to changes in human impact, such as deposition of atmospheric pollutants, fish stocking and climate change. The results were compared to occasional samplings performed in the 1940s, and to the remains found in sediment cores. When monitoring started, the most acid-sensitive of them, Lake Paione Superiore, was acidified, with evident effects in its flora and fauna: benthic diatoms assemblage was shif...

  15. Time-dependent corrosion fatique crack propagation in 7000 series aluminum alloys. M.S. Thesis

    Science.gov (United States)

    Mason, Mark E.

    1995-01-01

    The goal of this research is to characterize environmentally assisted subcritical crack growth for the susceptible short-longitudinal orientation of aluminum alloy 7075-T651, immersed in acidified and inhibited NaCl solution. This work is necessary in order to provide a basis for incorporating environmental effects into fatigue crack propagation life prediction codes such as NASA-FLAGRO (NASGRO). This effort concentrates on determining relevant inputs to a superposition model in order to more accurately model environmental fatigue crack propagation.

  16. 21 CFR 172.560 - Modified hop extract.

    Science.gov (United States)

    2010-04-01

    ..., ethyl alcohol, or isopropyl alcohol acidified with hydrochloric or sulfuric acid; oxidation with peracetic acid; isomerization by calcium chloride or magnesium chloride treatment in ethylene dichloride...

  17. Effects of afforestation and deforestation on the deposition, cycling and leaching of elements

    DEFF Research Database (Denmark)

    Rasmussen, L.

    1998-01-01

    forest, its input, cycling, turnover, and possible leaching is of crucial interest for forest management. The input of oxidised forms of nitrogen, together with sulphur, contributes to acidification of forest soils, but internal transformation processes, like nitrification, also contribute....... In parallel, changes in land use and management practice have contributed to changes in the cycling of elements and in soil conditions. Afforestation and deforestation can also change atmospheric dry deposition and the processes controlling the mobility of nutrients and acidifying substances. Different types...... of forest management such as choice of tree species, deforestation by clear-felling or selection forest, fertilization, liming, sludge and compost addition, etc. will influence the leaching of nutrients from forest ecosystems. Since nitrogen is assumed to be the most important macronutrient in European...

  18. Ion transporters involved in acidification of the resorption lacuna in osteoclasts

    DEFF Research Database (Denmark)

    Henriksen, K.; Sorensen, M.G.; Jensen, V.K.

    2008-01-01

    Osteoclasts possess a large amount of ion transporters, which participate in bone resorption; of these, the vacuolar-adenosine trisphosphatase (V-ATPase) and the chloride-proton antiporter ClC-7 acidify the resorption lacuna. However, whether other ion transporters participate in this process is ......, including carbonic anhydrase II, the NHEs, and potassium-chloride cotransporters, are all involved in resorption but do not seem to directly be involved in acidification of the lysosomes Udgivelsesdato: 2008/9......Osteoclasts possess a large amount of ion transporters, which participate in bone resorption; of these, the vacuolar-adenosine trisphosphatase (V-ATPase) and the chloride-proton antiporter ClC-7 acidify the resorption lacuna. However, whether other ion transporters participate in this process...

  19. Radiochemical determination of cesium-137 in seawater

    International Nuclear Information System (INIS)

    Cunha, I.I.L.; Munita, C.S.; Paiva, R.P.

    1990-01-01

    Seawater samples were collected from the Atlantic Ocean, in the vicinity of Ubatuba (Sao Paulo State - Brazil), acidified to pH 1 and stored in polyethylene containers. Cesium was precipitated with ammonium phospho molybdate (AMP), synthesized in our laboratory. The elements potassium and rubidium present in the seawater are also coprecipitated by AMP and adequate decontamination of the cesium is made by preparing a column by mixing Cs-137 AMP precipitate and asbestos. The interfering elements were eluted with 1.0 M ammonium nitrate solution whereas cesium was eluted with 1.0 M sodium hydroxide solution. Cesium was reprecipitated by acidifying the solution with concentrated hydrochloric acid. The overall chemical yield of cesium was of 75%. (author)

  20. Maternal resveratrol consumption and its programming effects on metabolic health in offspring mechanisms and potential implications.

    Science.gov (United States)

    Zheng, Sheng; Feng, Qianyun; Cheng, Jing; Zheng, Jia

    2018-04-27

    A growing body of evidence has clearly demonstrated that maternal nutrition can strongly determine the susceptibility to the development of metabolic diseases in offspring. With the increasing prevalence of maternal overweight, obesity, and gestational diabetes mellitus, it yields enormous burden for individual and public health. Interventions during pregnancy have been proven to be challenging, with limited efficacy and low compliance. Resveratrol, as a natural polyphenolic compound, has a wide-range of beneficial properties, including potent antiobesogenic, antiatherosclerotic, and antidiabetic effects. However, the role of maternal resveratrol intake on metabolic health in offspring has not been extensively investigated. Therefore, the aim of this study was to review the effects of maternal resveratrol supplementation on metabolic health in offspring and its potential mechanisms. © 2018 The Author(s).

  1. The subtle effects of sea water acidification on the amphipod Gammarus locusta

    Directory of Open Access Journals (Sweden)

    J. Williams

    2009-08-01

    Full Text Available We report an investigation of the effects of increases in pCO2 on the survival, growth and molecular physiology of the neritic amphipod Gammarus locusta which has a cosmopolitan distribution in estuaries. Amphipods were reared from juvenile to mature adult in laboratory microcosms at three different levels of pH in nominal range 8.1–7.6. Growth rate was estimated from weekly measures of body length. At sexual maturity the amphipods were sacrificed and assayed for changes in the expression of genes coding for a heat shock protein (hsp70 gene and the metabolic enzyme glyceraldehyde-3-phosphate dehydrogenase (gapdh gene. The data show that the growth and survival of this species is not significantly impacted by a decrease in sea water pH of up to 0.5 units. Quantitative real-time PCR analysis indicated that there was no significant effect of growth in acidified sea water on the sustained expression of the hsp70 gene. There was a consistent and significant increase in the expression of the gapdh gene at a pH of ~7.5 which, when combined with observations from other workers, suggests that metabolic changes may occur in response to acidification. It is concluded that sensitive assays of tissue physiology and molecular biology should be routinely employed in future studies of the impacts of sea water acidification as subtle effects on the physiology and metabolism of coastal marine species may be overlooked in conventional gross "end-point" studies of organism growth or mortality.

  2. Indirect effects of climate changes on cadmium bioavailability and biological effects in the Mediterranean mussel Mytilus galloprovincialis.

    Science.gov (United States)

    Nardi, Alessandro; Mincarelli, Luana Fiorella; Benedetti, Maura; Fattorini, Daniele; d'Errico, Giuseppe; Regoli, Francesco

    2017-02-01

    Despite the great interest in the consequences of climate change on the physiological functioning of marine organisms, indirect and interactive effects of rising temperature and pCO 2 on bioaccumulation and responsiveness to environmental pollutants are still poorly explored, particularly in terms of cellular mechanisms. According to future projections of temperature and pH/pCO 2 , this study investigated the main cellular pathways involved in metal detoxification and oxidative homeostasis in Mediterranean mussels, Mytilus galloprovincialis, exposed for 4 weeks to various combinations of two levels of pH/pCO 2 (8.2/∼400 μatm and 7.4/∼3000 μatm), temperature (20 and 25 °C), and cadmium addition (0 and 20 μg/L). Bioaccumulation was increased in metal exposed organisms but it was not further modulated by different temperature and pH/pCO 2 combinations. However, interactions between temperature, pH and cadmium had significant effects on induction of metallothioneins, responses of the antioxidant system and the onset of oxidative damages, which was tissue dependent. Multiple stressors increased metallothioneins concentrations in the digestive gland revealing different oxidative effects: while temperature and cadmium enhanced glutathione-dependent antioxidant protection and capability to neutralize peroxyl radicals, the metal increased the accumulation of lipid peroxidation products under acidified conditions. Gills did not reveal specific effects for different combinations of factors, but a general stress condition was observed in this tissue after various treatments. Significant variations of immune system were mainly caused by increased temperature and low pH, while co-exposure to acidification and cadmium enhanced metal genotoxicity and the onset of permanent DNA damage in haemocytes. Elaboration of the whole biomarker data in a cellular hazard index, corroborated the synergistic effects of temperature and acidification which increased the toxicological

  3. Electron transport in liquids: effect of unbalancing the methane molecule by deuteration

    International Nuclear Information System (INIS)

    Floriano, M.A.; Freeman, G.R.

    1983-01-01

    The mobility of electrons in nonpolar liquids is strongly dependent on the molecular shape. In spherelike methane the mobility is three orders of magnitude greater than in the rodlike ethane. To investigate the enormous gap between methane and ethane, the effect of slightly perturbing the symmetry of the methane molecule by progressive deuterium substitution was studied. At temperatures near the triple point (T/Tsub(c) approximately 0.48) and near the mobility maximum (T/Tsub(c) approximately 0.93) the mobilities change in the order: CH 4 > CH 3 D > CH 2 D 2 3 approximately CD 4 . The differences are related to the symmetries of the molecules and to the rotational motions. (Authors)

  4. Temperature effect on protection diode for plasma-process induced charging damage

    NARCIS (Netherlands)

    Wang, Zhichun; Scarpa, A.; Smits, Sander M.; Kuper, F.G.; Salm, Cora

    2002-01-01

    In this paper, the leakage current of different drain-well diodes for plasma-charging protection has been simulated at high temperature. The simulation shows that the high ambient temperature, especially during plasma deposition process, enormously enhances the efficacy of the protection diodes in

  5. Use of MgO to mitigate the effect of microbial CO2 production in the Waste Isolation Pilot Plant

    International Nuclear Information System (INIS)

    Wang, Y.; Brush, L.H.

    1997-01-01

    The Waste Isolation Pilot Plant (WIPP), located in a salt bed in southern New Mexico, is designed by US Department of Energy to demonstrate the safe and permanent disposal of design-basis transuranic waste. WIPP performance assessment requires consideration of radionuclide release in brines in the event of inadvertent human intrusion. The mobility of radionuclides depends on chemical factors such as brine pmH (-log molality of H + ) and CO 2 fugacity. According to current waste inventory estimates, a large quantity (∼ 10 9 moles C) of organic materials will be emplaced in the WIPP. Those organic material will potentially be degraded by halophilic or halotolerant microorganisms in the presence of liquid water in the repository, especially if a large volume of brine is introduced into the repository by human intrusions. Organic material biodegradation will produce a large amount of CO 2 , which will acidify the WIPP brine and thus significantly increase the mobility of actinides. This communication addresses (1) the rate of organic material biodegradation and the quantity of CO 2 to be possibly generated, (2) the effect of microbial CO 2 production on overall WIPP performance, and (3) the mechanism of using MgO to mitigate this effect

  6. Interactive effects of temperature, food and skeletal mineralogy mediate biological responses to ocean acidification in a widely distributed bryozoan.

    Science.gov (United States)

    Swezey, Daniel S; Bean, Jessica R; Ninokawa, Aaron T; Hill, Tessa M; Gaylord, Brian; Sanford, Eric

    2017-04-26

    Marine invertebrates with skeletons made of high-magnesium calcite may be especially susceptible to ocean acidification (OA) due to the elevated solubility of this form of calcium carbonate. However, skeletal composition can vary plastically within some species, and it is largely unknown how concurrent changes in multiple oceanographic parameters will interact to affect skeletal mineralogy, growth and vulnerability to future OA. We explored these interactive effects by culturing genetic clones of the bryozoan Jellyella tuberculata (formerly Membranipora tuberculata ) under factorial combinations of dissolved carbon dioxide (CO 2 ), temperature and food concentrations. High CO 2 and cold temperature induced degeneration of zooids in colonies. However, colonies still maintained high growth efficiencies under these adverse conditions, indicating a compensatory trade-off whereby colonies degenerate more zooids under stress, redirecting energy to the growth and maintenance of new zooids. Low-food concentration and elevated temperatures also had interactive effects on skeletal mineralogy, resulting in skeletal calcite with higher concentrations of magnesium, which readily dissolved under high CO 2 For taxa that weakly regulate skeletal magnesium concentration, skeletal dissolution may be a more widespread phenomenon than is currently documented and is a growing concern as oceans continue to warm and acidify. © 2017 The Author(s).

  7. Effect of irradiation on the pH for 11 commercial salad dressings near expiration date

    International Nuclear Information System (INIS)

    Sagretti, Juliana M.A.; Fabbri, Adriana D.T.; Hirashima, Fabiana K.; Nunes, Thaise C.F.; Rogovschi, Vladimir D.; Sabato, Susy F.

    2013-01-01

    The demand for salad dressings has evolved significantly in recent years and has become a frequent option in the meal. The pH values for salad dressings are low, precisely because they are emulsions acidified by adding ingredients, such as, acetic acid and / or citric acid and preservatives like benzoic acid and / or sorbic those provide an environment unfavorable to the growth of many microorganisms. However, the deterioration of such products occur as a result of growth of lactobacilli, bacilli, yeasts, once damaged, can present loss of taste and dark spots on the surface as an indication of yeast colony formation. Whereas, the positive effects of gamma irradiation on food, such as improved safety and stability of food products on shelves, the pH is a key factor for assessing not only the conservation, but also to the quality. The aim of this study was to reassess the effects of radiation on the pH of 9 varieties of commercial salad dressing after 6 months of storage, near the expiration of samples validity. The samples were acquired on the market of Sao Paulo and subjected to low doses of radiation at irradiator Multipurpose IPEN / CNEN-SP. The irradiated samples were compared to control. Statistical analyzes were performed using the program Graph Pad 5 and adopting a significance level of 5% (p <0.05). (author)

  8. Effect of irradiation on the pH for 11 commercial salad dressings near expiration date

    Energy Technology Data Exchange (ETDEWEB)

    Sagretti, Juliana M.A.; Fabbri, Adriana D.T.; Hirashima, Fabiana K.; Nunes, Thaise C.F.; Rogovschi, Vladimir D.; Sabato, Susy F., E-mail: jusagretti@uol.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    The demand for salad dressings has evolved significantly in recent years and has become a frequent option in the meal. The pH values for salad dressings are low, precisely because they are emulsions acidified by adding ingredients, such as, acetic acid and / or citric acid and preservatives like benzoic acid and / or sorbic those provide an environment unfavorable to the growth of many microorganisms. However, the deterioration of such products occur as a result of growth of lactobacilli, bacilli, yeasts, once damaged, can present loss of taste and dark spots on the surface as an indication of yeast colony formation. Whereas, the positive effects of gamma irradiation on food, such as improved safety and stability of food products on shelves, the pH is a key factor for assessing not only the conservation, but also to the quality. The aim of this study was to reassess the effects of radiation on the pH of 9 varieties of commercial salad dressing after 6 months of storage, near the expiration of samples validity. The samples were acquired on the market of Sao Paulo and subjected to low doses of radiation at irradiator Multipurpose IPEN / CNEN-SP. The irradiated samples were compared to control. Statistical analyzes were performed using the program Graph Pad 5 and adopting a significance level of 5% (p <0.05). (author)

  9. Towards the effective plastic waste management in Bangladesh: a review.

    Science.gov (United States)

    Mourshed, Monjur; Masud, Mahadi Hasan; Rashid, Fazlur; Joardder, Mohammad Uzzal Hossain

    2017-12-01

    The plastic-derived product, nowadays, becomes an indispensable commodity for different purposes. A huge amount of used plastic causes environmental hazards that turn in danger for marine life, reduces the fertility of soil, and contamination of ground water. Management of this enormous plastic waste is challenging in particular for developing countries like Bangladesh. Lack of facilities, infrastructure development, and insufficient budget for waste management are some of the prime causes of improper plastic management in Bangladesh. In this study, the route of plastic waste production and current plastic waste management system in Bangladesh have been reviewed extensively. It emerges that no technical and improved methods are adapted in the plastic management system. A set of the sustainable plastic management system has been proposed along with the challenges that would emerge during the implementation these strategies. Successful execution of the proposed systems would enhance the quality of plastic waste management in Bangladesh and offers enormous energy from waste.

  10. Effect of oxidoreduction potential and of gas bubbling on rheological properties and microstructure of acid skim milk gels acidified with glucono-delta-lactone.

    Science.gov (United States)

    Martin, F; Cayot, N; Marin, A; Journaux, L; Cayot, P; Gervais, P; Cachon, R

    2009-12-01

    Milk oxidoreduction potential was modified using gases during the production of a model dairy product and its effect on gel setting was studied. Acidification by glucono-delta-lactone was used to examine the physicochemistry of gelation and to avoid variations due to microorganisms sensitive to oxidoreduction potential. Four conditions of oxidoreduction potential were applied to milk: milk was gassed with air, nongassed, gassed with N(2), or gassed with N(2)H(2). The rheological properties and microstructure of these gels were determined using viscoelasticimetry, measurement of whey separation, and confocal laser scanning microscopy. It appeared that a reducing environment led to less-aggregated proteins within the matrix and consequently decreased whey separation significantly. The use of gas to modify oxidoreduction potential is a possible way to improve the quality of dairy products.

  11. Mixed-Species Effects on Soil C and N Stocks, C/N Ratio and pH Using a Transboundary Approach in Adjacent Common Garden Douglas-Fir and Beech Stands

    Directory of Open Access Journals (Sweden)

    Seid Muhie Dawud

    2017-03-01

    Full Text Available Mixed forest of Douglas-fir and beech has been suggested as one of the possible future forest types in Northwest Europe but the effects of this mixed forest on soil properties relative to monoculture stands are unknown. In a transboundary investigation of adjacent common garden Douglas-fir and beech stands, we determined the effects on topsoil properties. However, responses of C and N stocks, the C/N ratio and pH were site- and soil layer-specific and were mainly single-sided and without synergistic effects. Beech reduced the soil C and N stocks in Douglas-fir at the nutrient-poor site, caused an increase in the C/N ratio in the forest floor and mineral soil at both nutrient-poor and -rich sites, and reduced the acidifying effect of Douglas-fir at the nutrient-poor site. These results do not support the hypothesis that mixture effects would be consistent across sites and soil layers. The lack of synergistic effects may be attributed to the relatively similar litter quality or rooting depth that prevented any larger niche differentiation and complementarity. The results indicate that the transboundary approach within a mature common garden proved useful as a platform to test tree species interactions, and this approach could be explored in soil studies until dedicated mixed-species common gardens reach maturity.

  12. Fighting “zombie” health apps through K-Factor virality and other mobile gaming techniques – The application of commercial gaming techniques to create more effective mHealth solutions

    OpenAIRE

    Sorcha Moore

    2015-01-01

    Background Mobile today is recognised as being one of the highest value channels in the marketing mix . The potential for mobile applications to influence user behaviour and establish behavioural habits at scale is evidenced in apps such as Cow Clicker and Candy Crush attracting massive active audiences at their peak, and high levels of viral growth and user activity. mHealth has enormous potential to effect positive behavioural change for health. However with an estimated 80% of overall...

  13. Partitioning high-level waste from alkaline solution: A literature survey

    International Nuclear Information System (INIS)

    Marsh, S.F.

    1993-05-01

    Most chemical partitioning procedures are designed for acidic feed solutions. However, the high-level waste solutions in the underground storage tanks at US Department of Energy defense production sites are alkaline. Effective partitioning procedures for alkaline solutions could decrease the need to acidify these solutions and to dissolve the solids in acid, which would simplify subsequent processing and decrease the generation of secondary waste. The author compiles candidate technologies from his review of the chemical literature, experience, and personal contacts. Several of these are recommended for evaluation

  14. Radiation exposure assessment following the 1978 Church Rock uranium mill tailings spill

    International Nuclear Information System (INIS)

    Ruttenber, A.J. Jr.; Kreiss, K.

    1981-01-01

    Early in the morning of July 16, 1979, there was a breach in the earthen retaining dam of a tailings pond at the United Nuclear Corporation's (UNC's) Church Rock uranium mill. The acidified liquid and tailings slurry spilled through the damaged portion of the retaining wall into an arroyo that is a tributary to the Rio Puerco river system. This paper summarizes postspill monitoring efforts and relates the assessment of this spill to the general question of evaluating the health effects of nuclear fuel-cycle wastes

  15. The diverse effects of complex chromosome rearrangements and chromothripsis in cancer development

    NARCIS (Netherlands)

    De Pagter, Mirjam S.; Kloosterman, Wigard P.

    2015-01-01

    In recent years, enormous progress has been made with respect to the identification of somatic mutations that contribute to cancer development. Mutation types range from small substitutions to large structural genomic rearrangements, including complex reshuffling of the genome. Sets of mutations in

  16. Effect of four acidifying materials added to a calcareous soil on the availability of phosphorus to ryegrass

    Energy Technology Data Exchange (ETDEWEB)

    Sen Gupta, M B; Cornfield, A H

    1964-12-01

    Ryegrass was grown in a pot test using a calcareous soil (0.36% calcium carbonate) treated with sulfur, ammonium sulfate, ferrous sulfate, and aluminium sulfate at 0.1% sulfur-equivalent, with potassium nitrate added where necessary, including the control, to equalize nitrogen supply. The sulfur treatment was the only one which significantly increased dry matter yields, total phosphorus uptake and top/root ratios in dry matter yields and total phosphorus. The ammonium sulfate, ferrous sulfate, and aluminium sulfate treatments significantly reduced top/root ratios in dry-matter yields and total phosphorus. 6 references, 1 table.

  17. The effect of naturally acidified irrigation water on agricultural volcanic soils. The case of Asembagus, Java, Indonesia

    NARCIS (Netherlands)

    Los, A.M.D.; Vriend, S.P.; Bergen, M.J.; Gaans, R.F.M.

    2008-01-01

    Acid water from the Banyuputih river (pH similar to 3.5) is used for the irrigation of agricultural land in the Asembagus coastal area (East Java, Indonesia), with harmful consequences for rice yields. The river water has an unusual composition which is caused by seepage from the acidic Kawah Ijen

  18. Combined Effects of Ocean Warming and Acidification on Copepod Abundance, Body Size and Fatty Acid Content.

    Science.gov (United States)

    Garzke, Jessica; Hansen, Thomas; Ismar, Stefanie M H; Sommer, Ulrich

    2016-01-01

    Concerns about increasing atmospheric CO2 concentrations and global warming have initiated studies on the consequences of multiple-stressor interactions on marine organisms and ecosystems. We present a fully-crossed factorial mesocosm study and assess how warming and acidification affect the abundance, body size, and fatty acid composition of copepods as a measure of nutritional quality. The experimental set-up allowed us to determine whether the effects of warming and acidification act additively, synergistically, or antagonistically on the abundance, body size, and fatty acid content of copepods, a major group of lower level consumers in marine food webs. Copepodite (developmental stages 1-5) and nauplii abundance were antagonistically affected by warming and acidification. Higher temperature decreased copepodite and nauplii abundance, while acidification partially compensated for the temperature effect. The abundance of adult copepods was negatively affected by warming. The prosome length of copepods was significantly reduced by warming, and the interaction of warming and CO2 antagonistically affected prosome length. Fatty acid composition was also significantly affected by warming. The content of saturated fatty acids increased, and the ratios of the polyunsaturated essential fatty acids docosahexaenoic- (DHA) and arachidonic acid (ARA) to total fatty acid content increased with higher temperatures. Additionally, here was a significant additive interaction effect of both parameters on arachidonic acid. Our results indicate that in a future ocean scenario, acidification might partially counteract some observed effects of increased temperature on zooplankton, while adding to others. These may be results of a fertilizing effect on phytoplankton as a copepod food source. In summary, copepod populations will be more strongly affected by warming rather than by acidifying oceans, but ocean acidification effects can modify some temperature impacts.

  19. Reductive Catalytic Fractionation of Corn Stover Lignin

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, Eric M.; Katahira, Rui; Reed, Michelle; Resch, Michael G.; Karp, Eric M.; Beckham, Gregg T.; Román-Leshkov, Yuriy

    2016-12-05

    Reductive catalytic fractionation (RCF) has emerged as an effective biomass pretreatment strategy to depolymerize lignin into tractable fragments in high yields. We investigate the RCF of corn stover, a highly abundant herbaceous feedstock, using carbon-supported Ru and Ni catalysts at 200 and 250 degrees C in methanol and, in the presence or absence of an acid cocatalyst (H3PO4 or an acidified carbon support). Three key performance variables were studied: (1) the effectiveness of lignin extraction as measured by the yield of lignin oil, (2) the yield of monomers in the lignin oil, and (3) the carbohydrate retention in the residual solids after RCF. The monomers included methyl coumarate/ferulate, propyl guaiacol/syringol, and ethyl guaiacol/syringol. The Ru and Ni catalysts performed similarly in terms of product distribution and monomer yields. The monomer yields increased monotonically as a function of time for both temperatures. At 6 h, monomer yields of 27.2 and 28.3% were obtained at 250 and 200 degrees C, respectively, with Ni/C. The addition of an acid cocatalysts to the Ni/C system increased monomer yields to 32% for acidified carbon and 38% for phosphoric acid at 200 degrees C. The monomer product distribution was dominated by methyl coumarate regardless of the use of the acid cocatalysts. The use of phosphoric acid at 200 degrees C or the high temperature condition without acid resulted in complete lignin extraction and partial sugar solubilization (up to 50%) thereby generating lignin oil yields that exceeded the theoretical limit. In contrast, using either Ni/C or Ni on acidified carbon at 200 degrees C resulted in moderate lignin oil yields of ca. 55%, with sugar retention values >90%. Notably, these sugars were amenable to enzymatic digestion, reaching conversions >90% at 96 h. Characterization studies on the lignin oils using two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance and gel permeation chromatrography revealed

  20. Construction of g-C{sub 3}N{sub 4}/Al{sub 2}O{sub 3} hybrids via in-situ acidification and exfoliation with enhanced photocatalytic activity

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Xiao-jing [College of Science, Hebei University of Science and Technology, Shijiazhuang 050018 (China); State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012 (China); Liu, Chao [College of Gemmology and Material Technics, Hebei GEO University, Shijiazhuang 050031 (China); Li, Xu-li [College of Science, Hebei University of Science and Technology, Shijiazhuang 050018 (China); Li, Fa-tang, E-mail: lifatang@126.com [College of Science, Hebei University of Science and Technology, Shijiazhuang 050018 (China); Li, Yu-pei [College of Science, Hebei University of Science and Technology, Shijiazhuang 050018 (China); Zhao, Jun [College of Science, Hebei University of Science and Technology, Shijiazhuang 050018 (China); State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012 (China); Liu, Rui-hong [College of Science, Hebei University of Science and Technology, Shijiazhuang 050018 (China)

    2017-02-01

    Highlights: • Ultrathin g-C{sub 3}N{sub 4}/Al{sub 2}O{sub 3} hybrids are prepared via in-situ reaction. • The structure modification role of in-situ formed HNO{sub 3} for g-C{sub 3}N{sub 4} is found. • The ultrathin g-C{sub 3}N{sub 4} nanosheets are formed by the acidified melamine and Al(OH){sub 3}. • In-situ calcination of melamine and Al(OH){sub 3} benefits the contact of C{sub 3}N{sub 4} and Al{sub 2}O{sub 3}. • The activity of g-C{sub 3}N{sub 4}/Al{sub 2}O{sub 3} is 16.6 times that of pristine g-C{sub 3}N{sub 4} in degrading RhB. - Abstract: Homogeneous ultrathin g-C{sub 3}N{sub 4} nanosheets/Al{sub 2}O{sub 3} heterojunctions are synthesized using melamine and Al(NO{sub 3}){sub 3} via in-situ reaction and the following thermal polymerization approach. The in-situ reaction between melamine and Al(NO{sub 3}){sub 3} results in the existence of HNO{sub 3}-acidified melamine and Al(OH){sub 3} aggregates via the hydrolysis of Al(NO{sub 3}){sub 3}. After thermal polymerization, the aggregates are converted to g-C{sub 3}N{sub 4}/Al{sub 2}O{sub 3} composites. The thermal polymerization of acidified melamine and the support effect of aluminum hydroxide for g-C{sub 3}N{sub 4} during the calcination process lead to highly dispersed amrophous Al{sub 2}O{sub 3} on ultrathin g-C{sub 3}N{sub 4} nanosheets, which is beneficial for the separation of photogenerated electron-hole pairs in the heterojunction. The degradation rate for Rhodamine B (RhB) over the most activie sample is 16.6 times than that of pristine g-C{sub 3}N{sub 4} under visible light irradiation, which can be attributed to the high specific surface area, highly dispersion of amorphous Al{sub 2}O{sub 3} on ultrathin g-C{sub 3}N{sub 4} nanosheet, and the effective electrons transfer from g-C{sub 3}N{sub 4} to the amorphous Al{sub 2}O{sub 3}.

  1. The Effect of Digital Publishing on Technical Services in University Libraries

    Science.gov (United States)

    Hunter, Ben

    2013-01-01

    The past decade has brought enormous changes in scholarly communication, leading many libraries to undertake large-scale digital publishing initiatives. However, no study has investigated how technical services departments are changing to support these new services. Using change management as a theoretical framework, the investigator uses content…

  2. Observation of a physical matrix effect during cold vapour generation measurement of mercury in emissions samples

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Richard J.C., E-mail: richard.brown@npl.co.uk; Webb, William R.; Goddard, Sharon L.

    2014-05-01

    Highlights: • A matrix effect for CV-AFS measurement of mercury in emissions samples is reported. • This results from the different efficiencies of liberation of reduced mercury. • There is a good correlation between solution density and the size of the effect. • Several methods to overcome the bias are presented and discussed. - Abstract: The observation of a physical matrix effect during the cold vapour generation–atomic fluorescence measurement of mercury in emissions samples is reported. The effect is as a result of the different efficiencies of liberation of reduced mercury from solution as the matrix of the solution under test varies. The result of this is that peak area to peak height ratios decease as matrix concentration increases, passing through a minimum, before the ratio then increases as matrix concentration further increases. In the test matrices examined – acidified potassium dichromate and sodium chloride solutions – the possible biases caused by differences between the calibration standard matrix and the test sample matrix were as large as 2.8% (relative) representing peak area to peak height ratios for calibration standards and matrix samples of 45 and 43.75, respectively. For the system considered there is a good correlation between the density of the matrix and point of optimum liberation of dissolved mercury for both matrix types. Several methods employing matrix matching and mathematical correction to overcome the bias are presented and their relative merits discussed; the most promising being the use of peak area, rather than peak height, for quantification.

  3. Medical care and payment for diabetes in China: enormous threat and great opportunity.

    Directory of Open Access Journals (Sweden)

    Wenying Yang

    Full Text Available BACKGROUND: The Diabetes Impact Study followed up a large national population-based screening study to estimate the use of and expenditures for medical care caused by diabetes in China and to ascertain the use and cost of essential basic medicines and care. METHODS: In 2009-10, the study team interviewed 1482 adults with diabetes and 1553 adults with glucose tolerance in the normal range from population-based random samples at 12 sites in China. The response rate was 67%. FINDINGS: After adjusting for age, sex, and urban/rural location, people with diabetes received 1.93 times more days of inpatient treatment, 2.40 times more outpatient visits, and 3.35 times more medications than people with normal glucose tolerance (all p<0.05. Adjusted expenditures for medical care were 3.38 times higher among people with diabetes than among people with normal glucose tolerance (p<0.01, unadjusted 3.97. Persons who were diagnosed with ≥ 10 years prior to the survey paid 3.75 times as much for medical care as those with ≤ 5 years of diagnosed diabetes. Among persons with diabetes, 45.2% took medication to control blood sugar, 21.1% took an antihypertensive medicine, 22.4% took daily aspirin, and 1.8% took a statin. Over the three months before the interview, 46.1% of persons with diabetes recalled seeing a doctor, 48.9% recalled a blood pressure measurement, and 54.5% recalled a blood sugar test. Over the year preceding the interview, 32.1% recalled a retinal screening and 17.9% recalled a foot examination. CONCLUSIONS: In China, health care use and costs were dramatically higher for people with diabetes than for people with normal glucose tolerance and, in relative terms, much higher than in industrialized countries. Low-cost generic medicines that would reduce diabetes expenditures were not fully used.

  4. Medical care and payment for diabetes in China: enormous threat and great opportunity.

    Science.gov (United States)

    Yang, Wenying; Zhao, Wenhui; Xiao, Jianzhong; Li, Rui; Zhang, Ping; Kissimova-Skarbek, Katarzyna; Schneider, Erin; Jia, Weiping; Ji, Linong; Guo, Xiaohui; Shan, Zhongyan; Liu, Jie; Tian, Haoming; Chen, Li; Zhou, Zhiguang; Ji, Qiuhe; Ge, Jiapu; Chen, Gang; Brown, Jonathan

    2012-01-01

    The Diabetes Impact Study followed up a large national population-based screening study to estimate the use of and expenditures for medical care caused by diabetes in China and to ascertain the use and cost of essential basic medicines and care. In 2009-10, the study team interviewed 1482 adults with diabetes and 1553 adults with glucose tolerance in the normal range from population-based random samples at 12 sites in China. The response rate was 67%. After adjusting for age, sex, and urban/rural location, people with diabetes received 1.93 times more days of inpatient treatment, 2.40 times more outpatient visits, and 3.35 times more medications than people with normal glucose tolerance (all psugar, 21.1% took an antihypertensive medicine, 22.4% took daily aspirin, and 1.8% took a statin. Over the three months before the interview, 46.1% of persons with diabetes recalled seeing a doctor, 48.9% recalled a blood pressure measurement, and 54.5% recalled a blood sugar test. Over the year preceding the interview, 32.1% recalled a retinal screening and 17.9% recalled a foot examination. In China, health care use and costs were dramatically higher for people with diabetes than for people with normal glucose tolerance and, in relative terms, much higher than in industrialized countries. Low-cost generic medicines that would reduce diabetes expenditures were not fully used.

  5. Open day an enormous success more than 15 000 visitors came to DESY

    CERN Document Server

    2003-01-01

    "More than 15 000 visitors seized the opportunity to look behind the scenes at the Helmholtz research center DESY. Around 600 DESY employees were in action to present "their" research center, answer questions and care for the welfare of their guests" (1 page).

  6. Drivers and Effects of Enterprise Resource Planning Post-Implementation Learning

    Science.gov (United States)

    Chang, Hsiu-Hua; Chou, Huey-Wen

    2011-01-01

    The use of enterprise resource planning (ERP) systems has grown enormously since 1990, but the failure to completely learn how to use them continues to produce disappointing results. Today's rapidly changing business environment and the integrative applications of ERP systems force users to continuously learn new skills after ERP implementation.…

  7. Effect of a commercial anion dietary supplement on acid-base balance, urine volume, and urinary ion excretion in male goats fed oat or grass hay diets.

    Science.gov (United States)

    Stratton-Phelps, Meri; House, John K

    2004-10-01

    To determine whether feeding a commercial anionic dietary supplement as a urinary acidifier to male goats may be useful for management of urolithiasis. 8 adult sexually intact male Toggenburg, Saanen, and Nubian goats. Goats were randomly assigned by age-, breed-, and weight-matched pairs to an oat or grass hay diet that was fed for 12 days. On days 13 to 14 (early sample collection time before supplementation), measurements were made of blood and urine sodium, potassium, calcium, magnesium, chloride, phosphorus, and sulfur concentrations; blood and urine pH; urine production; and water consumption. During the next 28 days, the anionic dietary supplement was added to the oat and grass hay diets to achieve a dietary cation-anion difference of 0 mEq/100g of dry matter. Blood and urine samples were analyzed during dietary supplementation on days 12 to 13 (middle sample collection time) and 27 to 28 (late sample collection time). Blood bicarbonate, pH, and urine pH of goats fed grass hay and goats fed oat hay were significantly decreased during the middle and late sample collection times, compared with the early sample collection time. Water consumption and urine production in all goats increased significantly during the late sample collection time, compared with the early sample collection time. The anionic dietary supplement used in our study increases urine volume, alters urine ion concentrations, and is an efficacious urinary acidifier in goats. Goats treated with prolonged anionic dietary supplementation should be monitored for secondary osteoporosis from chronic urinary calcium loss.

  8. A fermented meat model system for studies of microbial aroma formation

    DEFF Research Database (Denmark)

    Tjener, Karsten; Stahnke, Louise Heller; Andersen, L.

    2003-01-01

    A fermented meat model system was developed, by which microbial formation of volatiles could be examined The model was evaluated against dry, fermented sausages with respect to microbial growth, pH and volatile profiles. Fast and slowly acidified sausages and models were produced using the starter......H, microbial growth and volatile profiles was similar to sausage production. Based on these findings, the model system was considered valid for studies of aroma formation of meat cultures for fermented sausage....... for multivariate data analysis. Growth of lactic acid bacteria was comparable for model and sausages, whereas survival of S. xylosus was better in the model. Multivariate analysis of volatiles showed that differences between fast and slowly acidified samples were identical for model and sausage. For both sausage...

  9. Application of enzymes for efficient extraction, modification, and development of functional properties of lime pectin

    DEFF Research Database (Denmark)

    Dominiak, Malgorzata Maria; Marie Søndergaard, Karen; Wichmann, Jesper

    2014-01-01

    pectin. The most efficient enzyme preparation was Laminex C2K derived from Penicillium funiculosum which, during 4 h treatment at pH 3.5, 50 °C, released pectin with similar yield (23% w/w), molecular weight (69 kDa), and functional properties e.g. gelling, stabilization of acidified milk drinks...... and viscosity as the classically acid-extracted pectins (8 h treatment at 70 °C, pH ... at higher temperatures. The Laminex CK2 extracted pectin polymers were not sensitive to the presence of Ca2+ ions, they formed a gel at low pH in the presence of sugar and were able to stabilize acidified milk drinks. Further modification by enzymatic de-esterification of the pectin extracted with Laminex C...

  10. Influence of heating and acidification on the flavor of whey protein isolate.

    Science.gov (United States)

    White, S S; Fox, K M; Jervis, S M; Drake, M A

    2013-03-01

    Previous studies have established that whey protein manufacture unit operations influence the flavor of dried whey proteins. Additionally, manufacturers generally instantize whey protein isolate (WPI; ≥ 90% protein) by agglomeration with lecithin to increase solubility and wettability. Whey protein isolate is often subjected to additional postprocessing steps in beverage manufacturing, including acidification and heat treatment. These postprocessing treatments may further influence formation or release of flavors. The objective of the first study was to characterize the effect of 2 processing steps inherent to manufacturing of acidic protein beverages (acidification and heat treatment) on the flavor of non-instant WPI. The second study sought to determine the effect of lecithin agglomeration, a common form of instantized (INST) WPI used in beverage manufacturing, on the flavor of WPI after acidification and heat treatment. In the first experiment, commercial non-instantized (NI) WPI were rehydrated and evaluated as is (control); acidified to pH 3.2; heated to 85°C for 5 min in a benchtop high temperature, short time (HTST) pasteurizer; or acidified to 3.2 and heated to 85°C for 30s (AH-HTST). In the second experiment, INST and NI commercial WPI were subsequently evaluated as control, acidified, heated, or AH-HTST. All samples were evaluated by descriptive sensory analysis, solid-phase microextraction (SPME), and gas chromatography-mass spectrometry. Acidification of NI WPI produced higher concentrations of dimethyl disulfide (DMDS) and sensory detection of potato/brothy flavors, whereas heating increased cooked/sulfur flavors. Acidification and heating increased cardboard, potato/brothy, and malty flavors and produced higher concentrations of aldehydes, ketones, and sulfur compounds. Differences between INST and NI WPI existed before treatment; INST WPI displayed cucumber flavors not present in NI WPI. After acidification, INST WPI were distinguished by higher

  11. Effective World Modeling: Multisensor Data Fusion Methodology for Automated Driving.

    Science.gov (United States)

    Elfring, Jos; Appeldoorn, Rein; van den Dries, Sjoerd; Kwakkernaat, Maurice

    2016-10-11

    The number of perception sensors on automated vehicles increases due to the increasing number of advanced driver assistance system functions and their increasing complexity. Furthermore, fail-safe systems require redundancy, thereby increasing the number of sensors even further. A one-size-fits-all multisensor data fusion architecture is not realistic due to the enormous diversity in vehicles, sensors and applications. As an alternative, this work presents a methodology that can be used to effectively come up with an implementation to build a consistent model of a vehicle's surroundings. The methodology is accompanied by a software architecture. This combination minimizes the effort required to update the multisensor data fusion system whenever sensors or applications are added or replaced. A series of real-world experiments involving different sensors and algorithms demonstrates the methodology and the software architecture.

  12. Higher Education Leadership: Servant Leadership and the Effects on Student Satisfaction

    Science.gov (United States)

    Padron, Joshua

    2012-01-01

    The enormous challenge confronting higher education today in relation to its revenue shortfall, lack of student persistence and low graduation rates are problematic. The literature proposes that if an institution of higher education increases student satisfaction rates, they will increase student persistence. Also, the literature suggests that an…

  13. Draft Whole-Genome Sequences of Three Lactobacillus plantarum Food Isolates

    NARCIS (Netherlands)

    Fernández Ramírez, Mónica D; Boekhorst, Jos; de Jong, Anne; Kuipers, Oscar P; Abee, Tjakko; Nierop Groot, Masja N

    2016-01-01

    Lactobacillus plantarum is a widespread member of the Lactobacillus genus and frequently isolated from spoiled acidified food products. Here, we report the draft genome sequences of three L. plantarum food isolates.

  14. Draft whole-genome sequences of three Lactobacillus plantarum food isolates

    NARCIS (Netherlands)

    Fernandez Ramirez, Monica; Boekhorst, Jos; Jong, de Anne; Kuipers, Oscar P.; Abee, Tjakko; Nierop Groot, Masja

    2016-01-01

    Lactobacillus plantarum is a widespread member of the Lactobacillus genus and frequently isolated from spoiled acidified food products. Here, we report the draft genome sequences of three L. plantarum food isolates.

  15. Characterization factors for terrestrial acidification at the global scale: a systematic analysis of spatial variability and uncertainty.

    Science.gov (United States)

    Roy, Pierre-Olivier; Azevedo, Ligia B; Margni, Manuele; van Zelm, Rosalie; Deschênes, Louise; Huijbregts, Mark A J

    2014-12-01

    Characterization factors (CFs) are used in life cycle assessment (LCA) to quantify the potential impact per unit of emission. CFs are obtained from a characterization model which assess the environmental mechanisms along the cause-effect chain linking an emission to its potential damage on a given area of protection, such as loss in ecosystem quality. Up to now, CFs for acidifying emissions did not cover the global scale and were only representative of their characterization model geographical scope. Consequently, current LCA practices implicitly assume that all emissions from a global supply chain occur within the continent referring to the characterization method geographical scope. This paper provides worldwide 2°×2.5° spatially-explicit CFs, representing the change in relative loss of terrestrial vascular plant species due to an emission change of nitrogen oxides (NOx), ammonia (NH3) and sulfur dioxide (SO2). We found that spatial variability in the CFs is much larger compared to statistical uncertainty (six orders of magnitude vs. two orders of magnitude). Spatial variability is mainly caused by the atmospheric fate factor and soil sensitivity factor, while the ecological effect factor is the dominant contributor to the statistical uncertainty. The CFs provided in our study allow the worldwide spatially explicit evaluation of life cycle impacts related to acidifying emissions. This opens the door to evaluate regional life cycle emissions of different products in a global economy. Copyright © 2014 Elsevier B.V. All rights reserved.

  16. Perceived Effect of Climate Variation on Food Crop Production in ...

    African Journals Online (AJOL)

    The study objective is to determine the perception of food crop farmers in Oyo state to climate variation as it affects their production, because the relationship between climate variation and food security is direct and Oyo State has enormous potentials to make Nigeria food secure. Multi-stage sampling technique was used to ...

  17. Effect of elevated manganese on the ultraviolet- and blue light-absorbing compounds of cucumber cotyledons and leaf tissues

    International Nuclear Information System (INIS)

    Caldwell, C.R.

    1998-01-01

    The effect of manganese [Mn(II)] on the pigments of cucumber (Cucumis sativus L., cv Poinsett 76) leaf and cotyledon tissues was investigated. Tissue disks (7 mm) were exposed to increasing Mn(II) concentrations from 100 micromolar to 2.5 mM. Acetone (carotenoid-rich fraction) and acidified methanol (flavonoid-rich fraction) extracts were analyzed by high performance liquid chromatography. Although none of the Mn(II)-treated tissues showed visible damage, Mn(II) at concentrations of 250 micromolar and above significantly reduced (60%) the beta-carotene levels of light-incubated leaf tissues. A major Mn(II)-induced, UV-absorbing compound was observed in methanol extracts of cotyledonary tissues exposed to Mn(II) in the dark. In leaf tissues, Mn(II) reduced the levels of certain UV-absorbing compounds under both light conditions. These results demonstrate that excess leaf Mn(II) can rapidly impair isoprenoid metabolism, altering tissue carotenoid composition. Furthermore, Mn(II) may also modify phenylpropanoid metabolism, changing the tissue flavonoid composition. Both situations could sensitize plant tissues to oxidative stresses, particularly enhanced solar UV-B radiation, and may reduce the nutritional quality of leafy vegetables

  18. Device and methods for processing biomass to biogas

    Energy Technology Data Exchange (ETDEWEB)

    1981-04-16

    In the title process, which gives high yields and can be used profitably in agriculture, biomass (e.g. manure, slaughterhouse wastes) is acidified prior to anaerobic gasification in a neutral alkaline medium.

  19. Technologically important properties of lactic acid bacteria isolated ...

    African Journals Online (AJOL)

    SERVER

    2007-07-18

    Jul 18, 2007 ... fast acidifying strains are good candidate in the dairy fer- ..... predicting the lytic behavior and proteolytic activity of lactococcal ... Food Flavour: Generation Analysis and Process Influence. ... Preparation and consumer.

  20. Acid deposition in the northern hemisphere

    Energy Technology Data Exchange (ETDEWEB)

    Longhurst, J.W.S.; Green, S.E.; Lee, D.S.

    1987-02-01

    Examines the phenomenon of acid deposition: the distribution and magnitude of sources and its actual and potential direct and indirect effects on soils, forests and other vegetation; wildlife, freshwaters, materials and health. The wide range of technological and other controls that are available to reduce the emissions of pollutants contributing to the phenomenon is also examined and includes pre-combustion control of pollutants, removal during combustion and post-combustion control. Also considered are political responses to acidification, acid deposition monitoring in the United Kingdom and the treatment of acidified areas.

  1. Carbonate chemistry of an in-situ free-ocean CO2 enrichment experiment (antFOCE) in comparison to short term variation in Antarctic coastal waters.

    Science.gov (United States)

    Stark, J S; Roden, N P; Johnstone, G J; Milnes, M; Black, J G; Whiteside, S; Kirkwood, W; Newbery, K; Stark, S; van Ooijen, E; Tilbrook, B; Peltzer, E T; Berry, K; Roberts, D

    2018-02-12

    Free-ocean CO 2 enrichment (FOCE) experiments have been deployed in marine ecosystems to manipulate carbonate system conditions to those predicted in future oceans. We investigated whether the pH/carbonate chemistry of extremely cold polar waters can be manipulated in an ecologically relevant way, to represent conditions under future atmospheric CO 2 levels, in an in-situ FOCE experiment in Antarctica. We examined spatial and temporal variation in local ambient carbonate chemistry at hourly intervals at two sites between December and February and compared these with experimental conditions. We successfully maintained a mean pH offset in acidified benthic chambers of -0.38 (±0.07) from ambient for approximately 8 weeks. Local diel and seasonal fluctuations in ambient pH were duplicated in the FOCE system. Large temporal variability in acidified chambers resulted from system stoppages. The mean pH, Ω arag and fCO 2 values in the acidified chambers were 7.688 ± 0.079, 0.62 ± 0.13 and 912 ± 150 µatm, respectively. Variation in ambient pH appeared to be mainly driven by salinity and biological production and ranged from 8.019 to 8.192 with significant spatio-temporal variation. This experiment demonstrates the utility of FOCE systems to create conditions expected in future oceans that represent ecologically relevant variation, even under polar conditions.

  2. Irrigation model of bleached Kraft mill wastewater through volcanic soil as a pollutants attenuation process.

    Science.gov (United States)

    Navia, R; Inostroza, X; Diez, M C; Lorber, K E

    2006-05-01

    An irrigation process through volcanic soil columns was evaluated for bleached Kraft mill effluent pollutants retention. The system was designed to remove color and phenolic compounds and a simple kinetic model for determining the global mass transfer coefficient and the adsorption rate constant was used. The results clearly indicate that the global mass transfer coefficient values (K(c)a) and the adsorption rate constants are higher for the irrigation processes onto acidified soil. This means that the pretreatment of washing the volcanic soil with an acid solution has a positive effect on the adsorption rate for both pollutant groups. The enhanced adsorption capacity is partially explained by the activation of the metal oxides present in the soil matrix during the acid washing process. Increasing the flow rate from 1.5 to 2.5 ml/min yielded higher (K(c)a) values and adsorption rate constants for both pollutant groups. For instance, regarding color adsorption onto acidified soil, there is an increment of 43% in the (K(c)a) value for the experiment with a flow rate of 2.5 ml/min. Increasing the porosity of the column from 0.55 to 0.59, yielded a decrease in the (K(c)a) values for color and phenolic compounds adsorption processes. Onto natural soil for example, these decreases reached 21% and 24%, respectively. Therefore, the (K(c)a) value is dependent on both the liquid-phase velocity (external resistance) and the soil fraction in the column (internal resistance); making forced convection and diffusion to be the main transport mechanisms involved in the adsorption process. Analyzing the adsorption rate constants (K(c)a)/m, phenolic compounds and color adsorption rates onto acidified soil of 2.25 x 10(-6) and 2.62 x 10(-6) l/mg min were achieved for experiment 1. These adsorption rates are comparable with other adsorption systems and adsorbent materials.

  3. The building paradigm shift and its effect on Western European housing stocks

    NARCIS (Netherlands)

    Thomsen, A.

    2011-01-01

    The 20th century saw an enormous worldwide growth of the housing stock. In particular the building boom after WW-II, during which the housing stock in most countries was multifolded, focussed the attention of the housing sector primarily to the planning and realisation of new construction; the

  4. Kinetic isotope effect in dehydration of ionic solids. II. The kinetics of dehydration of calcium oxalate monohydrate

    International Nuclear Information System (INIS)

    Manche, E.P.; Carroll, B.

    1977-01-01

    The kinetics of the isothermal dehydration of the protonated and deuterated monohydrate of calcium oxalate has been investigated at 120, 150, and 170 0 C. The rate of dehydration for these salts was found to be k/sub H//k/sub D/ = 1.025 +- 0.012. This result rules out the enormous kinetic isotope effect as given in the literature. An isotope effect of a few percent is not ruled out; this magnitude is in keeping with that found by Heinzinger in other dehydration processes. An estimated difference of about 150 cal/mol between the heat of desorption for H 2 O and D 2 O should have led to a ratio, k/sub h//k/sub D/ = 1.20. The smaller observed ratio has been explained on the basis of a compensation effect and may be considered an example of the Barclay--Butler correlation

  5. Increase in acidifying water in the western Arctic Ocean

    Science.gov (United States)

    Qi, Di; Chen, Liqi; Chen, Baoshan; Gao, Zhongyong; Zhong, Wenli; Feely, Richard A.; Anderson, Leif G.; Sun, Heng; Chen, Jianfang; Chen, Min; Zhan, Liyang; Zhang, Yuanhui; Cai, Wei-Jun

    2017-02-01

    The uptake of anthropogenic CO2 by the ocean decreases seawater pH and carbonate mineral aragonite saturation state (Ωarag), a process known as Ocean Acidification (OA). This can be detrimental to marine organisms and ecosystems. The Arctic Ocean is particularly sensitive to climate change and aragonite is expected to become undersaturated (Ωarag Pacific Winter Water transport, driven by an anomalous circulation pattern and sea-ice retreat, is primarily responsible for the expansion, although local carbon recycling and anthropogenic CO2 uptake have also contributed. These results indicate more rapid acidification is occurring in the Arctic Ocean than the Pacific and Atlantic oceans, with the western Arctic Ocean the first open-ocean region with large-scale expansion of `acidified’ water directly observed in the upper water column.

  6. Littoral macroinvertebrates of acidified lakes in the Bohemian Forest

    Czech Academy of Sciences Publication Activity Database

    Ungermanová, L.; Kolaříková, K.; Stuchlík, E.; Senoo, T.; Horecký, J.; Kopáček, Jiří; Chvojka, P.; Tátosová, J.; Bitušík, P.; Fjellheim, A.

    2014-01-01

    Roč. 69, č. 9 (2014), s. 1190-1201 ISSN 0006-3088 R&D Projects: GA ČR(CZ) GA526/09/0567; GA ČR(CZ) GA206/07/1200 Grant - others:EC(XE) EURO-LIMPACS GOCE-CT-2003-505540 Institutional support: RVO:60077344 Keywords : lakes * macroinvertebrates * acidification * recovery * forest catchment Subject RIV: DJ - Water Pollution ; Quality Impact factor: 0.827, year: 2014

  7. Diatom diversity in chronically versus episodically acidified adirondack streams

    Science.gov (United States)

    Passy, S.I.; Ciugulea, I.; Lawrence, G.B.

    2006-01-01

    The relationship between algal species richness and diversity, and pH is controversial. Furthermore, it is still unknown how episodic stream acidification following atmospheric deposition affects species richness and diversity. Here we analyzed water chemistry and diatom epiphyton dynamics and showed their contrasting behavior in chronically vs. episodically acidic streams in the Adirondack region. Species richness and diversity were significantly higher in the chronically acidic brown water stream, where organic acidity was significantly higher and the ratio of inorganic to organic monomeric aluminum significantly lower. Conversely, in the episodically acidic clear water stream, the inorganic acidity and pH were significantly higher and the diatom communities were very species-poor. This suggests that episodic acidification in the Adirondacks may be more stressful for stream biota than chronic acidity. Strong negative linear relationships between species diversity, Eunotia exigua, and dissolved organic carbon against pH were revealed after the influence of non-linear temporal trends was partialled out using a novel way of temporal modeling. ?? 2006 WILEY-VCH Verlag GmbH & Co. KGaA.

  8. Naturally acidified habitat selects for ocean acidification-tolerant mussels.

    Science.gov (United States)

    Thomsen, Jörn; Stapp, Laura S; Haynert, Kristin; Schade, Hanna; Danelli, Maria; Lannig, Gisela; Wegner, K Mathias; Melzner, Frank

    2017-04-01

    Ocean acidification severely affects bivalves, especially their larval stages. Consequently, the fate of this ecologically and economically important group depends on the capacity and rate of evolutionary adaptation to altered ocean carbonate chemistry. We document successful settlement of wild mussel larvae ( Mytilus edulis ) in a periodically CO 2 -enriched habitat. The larval fitness of the population originating from the CO 2 -enriched habitat was compared to the response of a population from a nonenriched habitat in a common garden experiment. The high CO 2 -adapted population showed higher fitness under elevated P co 2 (partial pressure of CO 2 ) than the non-adapted cohort, demonstrating, for the first time, an evolutionary response of a natural mussel population to ocean acidification. To assess the rate of adaptation, we performed a selection experiment over three generations. CO 2 tolerance differed substantially between the families within the F 1 generation, and survival was drastically decreased in the highest, yet realistic, P co 2 treatment. Selection of CO 2 -tolerant F 1 animals resulted in higher calcification performance of F 2 larvae during early shell formation but did not improve overall survival. Our results thus reveal significant short-term selective responses of traits directly affected by ocean acidification and long-term adaptation potential in a key bivalve species. Because immediate response to selection did not directly translate into increased fitness, multigenerational studies need to take into consideration the multivariate nature of selection acting in natural habitats. Combinations of short-term selection with long-term adaptation in populations from CO 2 -enriched versus nonenriched natural habitats represent promising approaches for estimating adaptive potential of organisms facing global change.

  9. 7 CFR 932.161 - Reports.

    Science.gov (United States)

    2010-01-01

    ... a form provided by the committee, for each week (Sunday through Saturday, or such other 7-day period...; (5) acidified; (6) Spanish olives; (7) Sicilian style olives; (8) Greek style olives; (9) olive oil...

  10. Causes, Effects, and Remedies in Conflict Management

    Directory of Open Access Journals (Sweden)

    Kamran Khan

    2017-05-01

    Full Text Available While workplace conflicts have been widely studied in the literature, this researchprovides a holistic view of the causes and effects of such, and how managers or amanagement can resolve the conflicts among their teams and organization througha detailed, multidimensional framework carried out on one of the biggest textilefirms of Pakistan. With an initial sample of 145 respondents, 37 questionnaireswere dropped because of invalid and incomplete answers; therefore, the studywas carried out on 108 respondents. Conflicts are a part of human nature, butmanagement should play an important role in dealing with these issues, as therecan be enormous chances of conflicts due to a diverse workforce. Conflict alsoresults in poor work performance and low productivity; therefore, it’s suggestedto create teams or groups which may encourage a competitive culture in theorganization. Additionally, a few remedies are identified, which may resolve someissues; managers must look at those techniques for a better culture. 

  11. Influence of Iron on Production of the Antibacterial Compound Tropodithietic Acid and Its Noninhibitory Analog in Phaeobacter inhibens

    DEFF Research Database (Denmark)

    D'Alvise, Paul W; Phippen, Christopher B W; Nielsen, Kristian Fog

    2016-01-01

    production is influenced by substrate components. High concentrations of ferric citrate, as present in marine broth, or other iron sources were required for production of antibacterially active TDA. However, when supernatants of noninhibitory, low-iron cultures of Phaeobacter inhibens were acidified......Tropodithietic acid (TDA) is an antibacterial compound produced by some Phaeobacter and Ruegeria spp. of the Roseobacter clade. TDA production is studied in marine broth or agar since antibacterial activity in other media is not observed. The purpose of this study was to determine how TDA......, antibacterial activity was detected in a bioassay. The absence of TDA in nonacidified cultures and the presence of TDA in acidified cultures were verified by liquid chromatography-high-resolution mass spectrometry. A noninhibitory TDA analog (pre-TDA) was produced by P. inhibens, Ruegeria mobilis F1926...

  12. Evidence for the gastric cytoprotective effect of centrally injected agmatine.

    Science.gov (United States)

    Zádori, Zoltán S; Tóth, Viktória E; Fehér, Ágnes; Philipp, Kirsch; Németh, József; Gyires, Klára

    2014-09-01

    Agmatine (decarboxylated arginine) exerts cytoprotective action in several tissues, such as in the brain, heart or kidneys, but there is still controversy over the effects of agmatine on the gastric mucosa. The aim of the present study was to reveal the potential gastroprotective action of agmatine by using an acid-independent ulcer model to clarify which receptors and peripheral factors are involved in it. Gastric mucosal damage was induced by acidified ethanol. Mucosal levels of calcitonin gene-related peptide (CGRP) and somatostatin were determined by radioimmunoassay. For analysis of gastric motor activity the rubber balloon method was used. It was found that agmatine given intracerebroventricularly (i.c.v., 0.044-220 nmol/rat) significantly inhibited the development of ethanol-induced mucosal damage, while in the case of intraperitoneal injection (0.001-50mg/kg i.p.) it had only a minor effect. The central gastroprotective action of agmatine was completely antagonized by mixed alpha2-adrenoceptor and imidazoline I1 receptor antagonists (idazoxan, efaroxan), but only partially by yohimbine (selective alpha2-adrenoceptor antagonist) and AGN 192403 (selective I1 receptor ligand, putative antagonist). It was also inhibited by the non-selective opioid-receptor antagonist naloxone and the selective δ-opioid receptor antagonist naltrindole, but not by β-funaltrexamine and nor-Binaltorphimine (selective μ- and κ-opioid receptor antagonists, respectively). Furthermore, the effect of agmatine was antagonized by bilateral cervical vagotomy and by pretreatment with indomethacin and NG-nitro-l-arginine. Agmatine also reversed the ethanol-induced reduction of gastric mucosal CGRP and somatostatin content, but did not have any significant effect on gastric motor activity. These results indicate that agmatine given centrally induces gastric cytoprotection, which is mediated by central imidazoline I1 receptors, alpha2-adrenoceptors and δ-opioid receptors. Activation of

  13. Combined Effects of Ocean Warming and Acidification on Copepod Abundance, Body Size and Fatty Acid Content.

    Directory of Open Access Journals (Sweden)

    Jessica Garzke

    Full Text Available Concerns about increasing atmospheric CO2 concentrations and global warming have initiated studies on the consequences of multiple-stressor interactions on marine organisms and ecosystems. We present a fully-crossed factorial mesocosm study and assess how warming and acidification affect the abundance, body size, and fatty acid composition of copepods as a measure of nutritional quality. The experimental set-up allowed us to determine whether the effects of warming and acidification act additively, synergistically, or antagonistically on the abundance, body size, and fatty acid content of copepods, a major group of lower level consumers in marine food webs. Copepodite (developmental stages 1-5 and nauplii abundance were antagonistically affected by warming and acidification. Higher temperature decreased copepodite and nauplii abundance, while acidification partially compensated for the temperature effect. The abundance of adult copepods was negatively affected by warming. The prosome length of copepods was significantly reduced by warming, and the interaction of warming and CO2 antagonistically affected prosome length. Fatty acid composition was also significantly affected by warming. The content of saturated fatty acids increased, and the ratios of the polyunsaturated essential fatty acids docosahexaenoic- (DHA and arachidonic acid (ARA to total fatty acid content increased with higher temperatures. Additionally, here was a significant additive interaction effect of both parameters on arachidonic acid. Our results indicate that in a future ocean scenario, acidification might partially counteract some observed effects of increased temperature on zooplankton, while adding to others. These may be results of a fertilizing effect on phytoplankton as a copepod food source. In summary, copepod populations will be more strongly affected by warming rather than by acidifying oceans, but ocean acidification effects can modify some temperature impacts.

  14. The effect of a giant wind farm on precipitation in a regional climate model

    International Nuclear Information System (INIS)

    Fiedler, B H; Bukovsky, M S

    2011-01-01

    The Weather Research and Forecasting (WRF) model is employed as a nested regional climate model to study the effect of a giant wind farm on warm-season precipitation in the eastern two-thirds of the USA. The boundary conditions for WRF are supplied by 62 years of NCEP/NCAR (National Center for Environmental Prediction/National Center for Atmospheric Research) global reanalysis. In the model, the presence of a mid-west wind farm, either giant or small, can have an enormous impact on the weather and the amount of precipitation for one season, which is consistent with the known sensitivity of long-term weather forecasts to initial conditions. The effect on climate is less strong. In the average precipitation of 62 warm seasons, there is a statistically significant 1.0% enhancement of precipitation in a multi-state area surrounding and to the south-east of the wind farm.

  15. Effective World Modeling: Multisensor Data Fusion Methodology for Automated Driving

    Directory of Open Access Journals (Sweden)

    Jos Elfring

    2016-10-01

    Full Text Available The number of perception sensors on automated vehicles increases due to the increasing number of advanced driver assistance system functions and their increasing complexity. Furthermore, fail-safe systems require redundancy, thereby increasing the number of sensors even further. A one-size-fits-all multisensor data fusion architecture is not realistic due to the enormous diversity in vehicles, sensors and applications. As an alternative, this work presents a methodology that can be used to effectively come up with an implementation to build a consistent model of a vehicle’s surroundings. The methodology is accompanied by a software architecture. This combination minimizes the effort required to update the multisensor data fusion system whenever sensors or applications are added or replaced. A series of real-world experiments involving different sensors and algorithms demonstrates the methodology and the software architecture.

  16. Effect of fire disturbances on soil respiration of Larix gmelinii Rupr ...

    African Journals Online (AJOL)

    The Da Xing'an Mountain is a key distribution area for Chinese boreal forests and is a fire-prone area. Frequent forest fires have influenced on the regional carbon cycle enormously, especially for the influence of soil respiration. Thus, understanding post-fire soil respiration is important in the study of the global carbon ...

  17. Optical Diode Effect at Spin-Wave Excitations of the Room-Temperature Multiferroic BiFeO_{3}.

    Science.gov (United States)

    Kézsmárki, I; Nagel, U; Bordács, S; Fishman, R S; Lee, J H; Yi, Hee Taek; Cheong, S-W; Rõõm, T

    2015-09-18

    Multiferroics permit the magnetic control of the electric polarization and the electric control of the magnetization. These static magnetoelectric (ME) effects are of enormous interest: The ability to read and write a magnetic state current-free by an electric voltage would provide a huge technological advantage. Dynamic or optical ME effects are equally interesting, because they give rise to unidirectional light propagation as recently observed in low-temperature multiferroics. This phenomenon, if realized at room temperature, would allow the development of optical diodes which transmit unpolarized light in one, but not in the opposite, direction. Here, we report strong unidirectional transmission in the room-temperature multiferroic BiFeO_{3} over the gigahertz-terahertz frequency range. The supporting theory attributes the observed unidirectional transmission to the spin-current-driven dynamic ME effect. These findings are an important step toward the realization of optical diodes, supplemented by the ability to switch the transmission direction with a magnetic or electric field.

  18. Developing Skills for Employability at the Secondary Level: Effective Models for Asia

    Science.gov (United States)

    Jayaram, Shubha; Engmann, Michelle

    2014-01-01

    Globally, enormous gains have been made towards the goal of universal primary education, leading to increased demands for secondary education. Consequently, more youth and young adults are now entering the formal and informal labour markets from the secondary level, which makes it important to ensure that secondary schools teach skills relevant to…

  19. Microbiological safety of mayonnaise, salad dressings, and sauces produced in the United States: a review.

    Science.gov (United States)

    Smittle, R B

    2000-08-01

    The literature on the death and survival of foodborne pathogens in commercial mayonnaise, dressing, and sauces was reviewed and statistically analyzed with emphasis on Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes. The absence of reports of foodborne illness associated directly with the consumption of commercially prepared acidic dressings and sauces is evidence of their safety. Salmonella, E. coli O157:H7, E. coli, L. monocytogenes, Staphylococcus aureus, and Yersinia enterocolitica die when inoculated into mayonnaise and dressings. Historically, mayonnaise and dressings have been exempt from the acidified food regulations and have justly deserved this status due primarily to the toxic effect of acetic and to a lesser extent lactic and citric acids. These organic acids are inimical to pathogenic bacteria and are effective natural preservatives with acetic being the most effective in killing pathogenic bacteria at the pH values encountered in these products. Statistical analysis on data reported in the literature shows that the most important and significant factor in destroying pathogenic bacteria is pH as adjusted with acetic acid followed by the concentration of acetic acid in the water phase. The reported highest manufacturing target pH for dressings and sauces is 4.4, which is below the 4.75 pKa of acetic acid and below the reported inhibitory pH of 4.5 for foodborne pathogens in the presence of acetic acid. The overall conclusion is that these products are very safe. They should remain exempt from the acidified food regulations providing adequate research has been done to validate their safety, and the predominant acid is acetic and reasonable manufacturing precautions are taken.

  20. Effective Geothermal Utilisation close to the surface by the TT-Geothermal Radial Drilling (GRD-Method

    Directory of Open Access Journals (Sweden)

    Hans-Joachim Bayer

    2007-01-01

    Full Text Available In the late 1970-Years, Tracto-Technik developped a very effective radial-shaped percussion system for a geothermal heating, the ECOtherm-System, which was very well accepted by customers. Nowadays, a radial-shaped drilling system, operating some decameters below the surface, was developped by Tracto-Technik, which offers the chance of a very effective drilling for the use of geothermal energy. The main advantage of this development is the reduction of drilling costs by new constructions and new handling possibilities. Drilling processes like the rod connecting or the drill-hole enlargement were solved in other ways as usual, by very time-shortening and effective ways, which are presented in the paper. The new TT-Geothermal radial drilling methods need only a very small but highly effective drilling unit, which reduces the operational drilling cost in a enormous way. All operational drilling steps are reduced to less than a half time as usual. By these GRD-methods, the use of surface-close geothermal energy is simplified and less expansive.

  1. Photochemical, chemical, and biological transformations of dissolved organic carbon and its effect on alkalinity production in acidified lakes

    Czech Academy of Sciences Publication Activity Database

    Kopáček, Jiří; Hejzlar, Josef; Kaňa, Jiří; Porcal, Petr; Klementová, Š.

    2003-01-01

    Roč. 48, č. 1 (2003), s. 106-117 ISSN 0024-3590 R&D Projects: GA ČR GA206/00/0063 Institutional research plan: CEZ:AV0Z6017912 Keywords : organic matter * pH * metals Subject RIV: DJ - Water Pollution ; Quality Impact factor: 3.329, year: 2003

  2. Scenario modeling potential eco-efficiency gains from a transition to organic agriculture: life cycle perspectives on Canadian canola, corn, soy, and wheat production.

    Science.gov (United States)

    Pelletier, N; Arsenault, N; Tyedmers, P

    2008-12-01

    We used Life Cycle Assessment to scenario model the potential reductions in cumulative energy demand (both fossil and renewable) and global warming, acidifying, and ozone-depleting emissions associated with a hypothetical national transition from conventional to organic production of four major field crops [canola (Brassica rapa), corn (Zea mays), soy (Glycine max), and wheat (Triticum aestivum)] in Canada. Models of these systems were constructed using a combination of census data, published values, and the requirements for organic production described in the Canadian National Organic Standards in order to be broadly representative of the similarities and differences that characterize these disparate production technologies. Our results indicate that organic crop production would consume, on average, 39% as much energy and generate 77% of the global warming emissions, 17% of the ozone-depleting emissions, and 96% of the acidifying emissions associated with current national production of these crops. These differences were almost exclusively due to the differences in fertilizers used in conventional and organic farming and were most strongly influenced by the higher cumulative energy demand and emissions associated with producing conventional nitrogen fertilizers compared to the green manure production used for biological nitrogen fixation in organic agriculture. Overall, we estimate that a total transition to organic production of these crops in Canada would reduce national energy consumption by 0.8%, global warming emissions by 0.6%, and acidifying emissions by 1.0% but have a negligible influence on reducing ozone-depleting emissions.

  3. Chemical Reaction between Boric Acid and Phosphine Indicates Boric Acid as an Antidote for Aluminium Phosphide Poisoning

    Directory of Open Access Journals (Sweden)

    Motahareh Soltani

    2016-08-01

    Full Text Available Objectives: Aluminium phosphide (AlP is a fumigant pesticide which protects stored grains from insects and rodents. When it comes into contact with moisture, AlP releases phosphine (PH3, a highly toxic gas. No efficient antidote has been found for AlP poisoning so far and most people who are poisoned do not survive. Boric acid is a Lewis acid with an empty p orbital which accepts electrons. This study aimed to investigate the neutralisation of PH3 gas with boric acid. Methods: This study was carried out at the Baharlou Hospital, Tehran University of Medical Sciences, Tehran, Iran, between December 2013 and February 2014. The volume of released gas, rate of gas evolution and changes in pH were measured during reactions of AlP tablets with water, acidified water, saturated boric acid solution, acidified saturated boric acid solution, activated charcoal and acidified activated charcoal. Infrared spectroscopy was used to study the resulting probable adduct between PH3 and boric acid. Results: Activated charcoal significantly reduced the volume of released gas (P <0.01. Although boric acid did not significantly reduce the volume of released gas, it significantly reduced the rate of gas evolution (P <0.01. A gaseous adduct was formed in the reaction between pure AlP and boric acid. Conclusion: These findings indicate that boric acid may be an efficient and non-toxic antidote for PH3 poisoning.

  4. Effects of elevated carbon dioxide (CO2) concentrations on early developmental stages of the marine copepod Calanus finmarchicus Gunnerus (Copepoda: Calanoidae).

    Science.gov (United States)

    Pedersen, Sindre Andre; Våge, Vegard Thorset; Olsen, Anders Johny; Hammer, Karen Marie; Altin, Dag

    2014-01-01

    Ocean acidification poses an ongoing threat to marine organisms, and early life stages are believed to be particularly sensitive. The boreal calanoid copepod Calanus finmarchicus seasonally dominates the standing stock of zooplankton in the northern North Sea and North Atlantic, and due to its size and abundance is considered an ecological key species linking energy from primary producers to higher trophic levels. To examine whether the early stages of C. finmarchicus are particularly vulnerable to elevated levels of CO2, eggs and nauplii were subjected to different levels of CO2-acidified seawater for 1 wk. The first experiment, with eggs as the starting point, revealed no marked effect on hatching success, but a significant reduction in nauplii survival during incubation at 8800 ppm CO2. In addition, a significant decrease in ontogenetic development rate during incubation at 8800 ppm CO2 was observed in this experiment. In the second experiment, where third-stage nauplii represented the starting point, no significant effects on ontogenetic development and survival following exposure to pCO2 ≥ 7700 ppm were observed. Data suggest that the two first nauplii stages, which are fed endogenously, may be more vulnerable and therefore likely to represent the "bottleneck" for this species in a more acidic ocean. However, the absence of significant effects in the most sensitive stages during exposure to 2800 ppm CO2, a level that is well above worst-case scenario predictions for year 2300 (approximately 2000 ppm CO2), suggests that this species may be generally robust to direct effects of ocean acidification.

  5. Population Heterogeneity of Lactobacillus plantarum WCFS1 Microcolonies in Response to and Recovery from Acid Stress

    NARCIS (Netherlands)

    Ingham, C.J.; Beerthuyzen, M.; Vlieg, J.E.T.V.H.

    2008-01-01

    Within an isogenic microbial population in a homogenous environment, individual bacteria can still exhibit differences in phenotype. Phenotypic heterogeneity can facilitate the survival of subpopulations under stress. As the gram-positive bacterium Lactobacillus plantarum grows, it acidifies the

  6. Metadata: JPST000055 [jPOST repository metadata[Archive

    Lifescience Database Archive (English)

    Full Text Available ell debris, and concentrated up to ~45-fold. Concentrated media were acidified by acetate acidic buffer...ced, alkylated, and digested by trypsin. Digested peptides were purified using C18 column, concentrated, diluted with coupling buffer

  7. Effective search for stable segregation configurations at grain boundaries with data-mining techniques

    Science.gov (United States)

    Kiyohara, Shin; Mizoguchi, Teruyasu

    2018-03-01

    Grain boundary segregation of dopants plays a crucial role in materials properties. To investigate the dopant segregation behavior at the grain boundary, an enormous number of combinations have to be considered in the segregation of multiple dopants at the complex grain boundary structures. Here, two data mining techniques, the random-forests regression and the genetic algorithm, were applied to determine stable segregation sites at grain boundaries efficiently. Using the random-forests method, a predictive model was constructed from 2% of the segregation configurations and it has been shown that this model could determine the stable segregation configurations. Furthermore, the genetic algorithm also successfully determined the most stable segregation configuration with great efficiency. We demonstrate that these approaches are quite effective to investigate the dopant segregation behaviors at grain boundaries.

  8. Inhibition of intergranular stress corrosion cracking of sensitized type 304 stainless steel. Annual report

    International Nuclear Information System (INIS)

    Brown, B.F.

    1977-01-01

    The effectiveness of various inhibitors in mitigating stress corrosion cracking of stainless steel in hot aqueous environment was evaluated. The inhibitors studied were of three types: poly-oxy-anions, organic competitive absorbers, and simple cations; the corrosive medium was 4M NaCl acidified with H 2 SO 4 to ph of about 2.3. The following conclusions were reached: pH does not affect cracking kinetics in a sensitive way; cracking time is highly dependent on chloride concentrations; poly-oxy-anions do not perform well; organics offer some possibilities as inhibitors; cationic additives can have effects varying from trivial to total suppression of cracking--behavior is both cation and concentration dependent. 2 figures, 5 tables

  9. Reaction of Bullfrog tuff with J-13 well water at 900C and 1500C

    International Nuclear Information System (INIS)

    Oversby, V.M.; Knauss, K.G.

    1983-01-01

    A series of experiments was conducted on crushed tuff at 90 0 C and 150 0 C and on core wafer samples at 150 0 C. The results show the following: increasing the ratio of rock to water increases the rate of approach to steady-state concentrations in solution. Surface outcrop samples of Bullfrog tuff contain a minor component of highly soluble material believed to be a residue from the evaporation of surface runoff water in the pores of the rock. This material can be removed by shaking the crushed rock with water at room temperature and subjecting it briefly to heat with fresh water. Solution analyses for unfiltered samples that have reacted for short periods show higher concentrations of Al and Fe than do analyses for filtered samples; results for other elements are independent of filtration. This difference probably exists because of particulate matter in the solutions that dissolves when the samples are acidified prior to analysis. Agitation of samples during reaction produces sub-0.1 μ particles in the solutions. These particles dissolve when samples are acidified, resulting in abnormally high concentration values for some elements, such as Al and Fe. Comparison of the results for crushed rock with those for core wafers shows that the method of sample preparation does not have a large effect on the results of rock-water interaction studies

  10. Responses of calcification of massive and encrusting corals to past, present, and near-future ocean carbon dioxide concentrations

    International Nuclear Information System (INIS)

    Iguchi, Akira; Kumagai, Naoki H.; Nakamura, Takashi; Suzuki, Atsushi; Sakai, Kazuhiko; Nojiri, Yukihiro

    2014-01-01

    Highlights: • Growth rates of two corals in the acidified seawater were evaluated. • Highest growth rates were observed in pre-industrial pCO 2 level. • The growth rates also decreased in the near-future ocean acidification level. • The growth responses were affected by variations of parameters of carbon chemistry. • Bayesian modeling approach was effective for the inference of the best model. - Abstract: In this study, we report the acidification impact mimicking the pre-industrial, the present, and near-future oceans on calcification of two coral species (Porites australiensis, Isopora palifera) by using precise pCO 2 control system which can produce acidified seawater under stable pCO 2 values with low variations. In the analyses, we performed Bayesian modeling approaches incorporating the variations of pCO 2 and compared the results between our modeling approach and classical statistical one. The results showed highest calcification rates in pre-industrial pCO 2 level and gradual decreases of calcification in the near-future ocean acidification level, which suggests that ongoing and near-future ocean acidification would negatively impact coral calcification. In addition, it was expected that the variations of parameters of carbon chemistry may affect the inference of the best model on calcification responses to these parameters between Bayesian modeling approach and classical statistical one even under stable pCO 2 values with low variations

  11. Enormous achievement of the wind power in India; Indo no fusha no osorubeki jitsuryoku

    Energy Technology Data Exchange (ETDEWEB)

    Ida, H

    1996-10-27

    This paper presents the situation of the wind power generation in India. Indian wind turbine capacity sums to 600MW as the 4th power in the world. The financial combine NEPC has erected 3/4 of the total capacity. The joint enterprise NEPC-MYCON of NEPC and Danish wind turbine manufacturer imports main bodies, and produces only towers in India. The enterprise erected 1,500 systems (450MW) in 1989-95 receiving benefit from cheaper real estate and labor costs. As the author visited the wind farm including 10 250kW class wind power generation systems erected in 1994, wind velocity was a maximum of 6.3m/s, however, it is 10m/s on average in the monsoon period from April to July. The blades of wind turbines under erection are made of fiber reinforced plastics water-proofed by polyester resin. Generated power is sold to local governments, or wind turbines are sold to various private enterprises for private power generation or to local governments. Erection cost can be collected with power sales benefit by 6 years. Since power supply capability in India is only 40% of power demand, low-cost wind power generation is promising. 5 figs.

  12. The bacterial-fungal energy channel concept challenged by enormous functional versatility of soil protists

    NARCIS (Netherlands)

    Geisen, Stefan

    2016-01-01

    Abstract Protists (=protozoa) are commonly treated as bacterivores that control the bacterial energy channel in soil food webs. This ecologist’s perspective is, however, challenged by taxonomic studies showing that a range of protists feed on fungi, other protists and even nematodes. Recently, it

  13. The bacterial-fungal energy channel concept challenged by enormous functional versatility of soil protists

    NARCIS (Netherlands)

    Geisen, Stefan

    2016-01-01

    Protists (=protozoa) are commonly treated as bacterivores that control the bacterial energy channel in soil food webs. This ecologist’s perspective is, however, challenged by taxonomic studies showing that a range of protists feed on fungi, other protists and even nematodes. Recently, it was

  14. Diabetes-Specific Nutrition Algorithm: A Transcultural Program to Optimize Diabetes and Prediabetes Care

    NARCIS (Netherlands)

    Mechanick, Jeffrey I.; Marchetti, Albert E.; Apovian, Caroline; Benchimol, Alexander Koglin; Bisschop, Peter H.; Bolio-Galvis, Alexis; Hegazi, Refaat A.; Jenkins, David; Mendoza, Enrique; Sanz, Miguel Leon; Sheu, Wayne Huey-Herng; Tatti, Patrizio; Tsang, Man-Wo; Hamdy, Osama

    2012-01-01

    Type 2 diabetes (T2D) and prediabetes have a major global impact through high disease prevalence, significant downstream pathophysiologic effects, and enormous financial liabilities. To mitigate this disease burden, interventions of proven effectiveness must be used. Evidence shows that nutrition

  15. COTS – Harsh Condition Effects Considerations from Technology to User Level

    Directory of Open Access Journals (Sweden)

    Kirsten Weide-Zaage

    2017-08-01

    Full Text Available Radiation hardened devices are mostly extremely expensive. The continuously downscaling of microelectronic structures and the unavoidable presence of particle radiation on ground and in space leads to unwanted failures in electronic devices. Furthermore it is expected that in the next few years around 8000 new satellites will be launched around the world. Due to the enormous increasing need for Rad-Hard devices, there will be more focus on Commercial Of The Shelf (COTS devices, which costs are lower. Also nowadays microelectronics for automotive systems are tested to withstand radiation especially SEU-single event upsets. It is clear that SEU cannot be ignored anymore especially in the application of unmanned autonomous vehicles and systems. Reliability testing is expensive and extremely time consuming. The use of COTS-Commercials of the shelf is the ultimate goal to reach. In this paper, an overview of radiation effects on different CMOS technologies used in COTS devices is given. These effects can be considered while selecting different functional equivalent COTS devices implemented with different technologies. Moreover, an overview of software techniques used in programmable commercial devices to reduce the radiation effects is also described.

  16. Patterned electrochemical deposition of copper using an electron beam

    Directory of Open Access Journals (Sweden)

    Mark den Heijer

    2014-02-01

    Full Text Available We describe a technique for patterning clusters of metal using electrochemical deposition. By operating an electrochemical cell in the transmission electron microscope, we deposit Cu on Au under potentiostatic conditions. For acidified copper sulphate electrolytes, nucleation occurs uniformly over the electrode. However, when chloride ions are added there is a range of applied potentials over which nucleation occurs only in areas irradiated by the electron beam. By scanning the beam we control nucleation to form patterns of deposited copper. We discuss the mechanism for this effect in terms of electron beam-induced reactions with copper chloride, and consider possible applications.

  17. Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region

    DEFF Research Database (Denmark)

    Madsen, Mette; Møller, Holger J; Nielsen, Marianne Jensby

    2004-01-01

    binding to SRCR domain 3 exhibited effective inhibition of ligand binding. Furthermore, analysis of purified native CD163 revealed that proteolytic cleavage in SRCR domain 3 inactivates ligand binding. Calcium protects against cleavage in this domain. Analysis of the calcium sensitivity of ligand binding...... to CD163 demonstrated that optimal ligand binding requires physiological plasma calcium concentrations, and an immediate ligand release occurs at the low calcium concentrations measured in acidifying endosomes. In conclusion, SRCR domain 3 of CD163 is an exposed domain and a critical determinant...... for the calcium-sensitive coupling of haptoglobin.hemoglobin complexes....

  18. Effects of acidification on olfactory-mediated behaviour in freshwater and marine ecosystems: a synthesis.

    Science.gov (United States)

    Leduc, Antoine O H C; Munday, Philip L; Brown, Grant E; Ferrari, Maud C O

    2013-01-01

    For many aquatic organisms, olfactory-mediated behaviour is essential to the maintenance of numerous fitness-enhancing activities, including foraging, reproduction and predator avoidance. Studies in both freshwater and marine ecosystems have demonstrated significant impacts of anthropogenic acidification on olfactory abilities of fish and macroinvertebrates, leading to impaired behavioural responses, with potentially far-reaching consequences to population dynamics and community structure. Whereas the ecological impacts of impaired olfactory-mediated behaviour may be similar between freshwater and marine ecosystems, the underlying mechanisms are quite distinct. In acidified freshwater, molecular change to chemical cues along with reduced olfaction sensitivity appear to be the primary causes of olfactory-mediated behavioural impairment. By contrast, experiments simulating future ocean acidification suggest that interference of high CO2 with brain neurotransmitter function is the primary cause for olfactory-mediated behavioural impairment in fish. Different physico-chemical characteristics between marine and freshwater systems are probably responsible for these distinct mechanisms of impairment, which, under globally rising CO2 levels, may lead to strikingly different consequences to olfaction. While fluctuations in pH may occur in both freshwater and marine ecosystems, marine habitat will remain alkaline despite future ocean acidification caused by globally rising CO2 levels. In this synthesis, we argue that ecosystem-specific mechanisms affecting olfaction need to be considered for effective management and conservation practices.

  19. The Effects of Academic, Behavioral and Organizational Factors on Mathematics Achievement in Secondary Schools

    Science.gov (United States)

    Perez, Miguel S.

    2013-01-01

    The nation's generation of "Baby Boomers" is now the grandparents of a new generation of students known as "Gen Y" and "Gen Z," respectively. "Gen X," parents of our current students, have the enormous task of raising their children as "digital natives" in a technological-savvy world. The idea of…

  20. Air pollution in northern Sweden. Deposition, levels and effects Oct 1994 - Sep 1995; Luftfoeroreningar i norra Sverige. Nedfall, halter och effekter oktober 1994 - september 1995

    Energy Technology Data Exchange (ETDEWEB)

    Hallgren-Larsson, E; Sjoeberg, K; Westling, O

    1996-06-01

    The purpose of the monitoring programme is to quantify sulphur and nitrogen deposition to forests, and to illustrate possible acidification of the soil. Deposition is investigated by precipitation studies in open field areas and as throughfall. Soil water chemistry in the forest stands is used as indicator of soil conditions. Air concentrations of sulphur dioxide, nitrogen dioxide, and ammonia were measured at two sites in the county of Jaemtland. This report concerns the hydrological year from October 1994 through September 1995 for 17 locations in the four most northern counties of Sweden. The results are compared with previous years and other regions in Sweden. The results shows a gradient with more precipitation, higher concentrations and larger deposition of sulphur and nitrogen at coastal than at inland locations. Mean from all 17 locations were pH 4.8 and sulphur concentration 0.4 mg/l (SO{sub 4}-S{sub ex}). Concentrations of nitrogen as nitrate and as ammonium were about 0.2 mg/l each. About half of the locations received larger amounts than target loads set for northern Sweden. Deposition of sulphur decreased in the county of Norrbotten during the last 4 years. Greater loads of sulphur and nitrogen were deposited in southern than in northern Sweden. The most acidified soil solutions (pH 4.5-4.6) with highest concentrations of aluminium were found at coastal locations outside Luleaa and Umeaa. At many locations in southern Sweden acidified soil solutions, rich in aluminium and poor in base cations, were detected. Similar conditions were found at two coastal locations in northern Sweden. Highest air concentrations of sulphur dioxide were found during January 1995. Nitrogen dioxide concentrations were highest during November 1994 through March 1995. A gradient of air concentrations of sulphur dioxide was found, with decreasing concentrations from southern to northern Sweden. 15 refs, 57 figs, 11 tabs

  1. Biogenic versus abiogenic emissions from agriculture in the Netherlands and options for emission control in tomato cultivation

    NARCIS (Netherlands)

    Pluimers, J.C.; Kroeze, C.; Bakker, E.J.; Challa, H.; Hordijk, L.

    2001-01-01

    In this paper, present-day emissions of greenhouse gases and acidifying compounds from agriculture are analysed at the farm level. Quantitative estimates are given for these emissions from three nested systems in the Netherlands: the agricultural sector, greenhouse horticulture, and tomato

  2. Indicators: Acidification

    Science.gov (United States)

    Acidification is a broad term that refers to the process by which aquatic ecosystems become more acidic. Acid rain and acid mine drainage are major sources of acidifying compounds, lowering the pH below the range where most living organisms function.

  3. Development of a flat membrane based device for electromembrane extraction

    DEFF Research Database (Denmark)

    Huang, Chuixiu; Eibak, Lars Erik Eng; Gjelstad, Astrid

    2014-01-01

    this EME device, exhaustive extraction of the basic drugs quetiapine, citalopram, amitriptyline, methadone and sertraline was investigated from both acidified water samples and human plasma. The volume of acceptor solution, extraction time, and extraction voltage were found to be important factors...

  4. Microbial biocatalytic preparation of 2-furoic acid by oxidation of 2 ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-05-18

    May 18, 2009 ... Growth experiments and biotransformation with N. corallina were performed in a ... The reaction mixture was acidified to pH 1 with 0.5 M HCl, then saturated with ... (aeration rate), can be correlated with the microbial growth.

  5. Impact of feed withdrawal and addition of acetic acid in drinking ...

    African Journals Online (AJOL)

    ALI GILANI

    2012-11-02

    Nov 2, 2012 ... 1.5, 3, 4.5 or 6% acetic acid added to their drinking water with feed ... contents of birds with acidified water in comparison to the control and feed withdrawal treatments. .... more hygienic evisceration process or lower microbial.

  6. Neutralization of wastewater from nitrite passivation

    International Nuclear Information System (INIS)

    Pawlowski, L.; Mientki, B.; Wasag, H.

    1982-01-01

    A method for neutralization of wastewater formed in nitrite passivation has been presented. The method consists of introducing urea into wastewater and acidifying it with sulphuric acid. Wastewater is neutralized with lime. After clarification, wastewater can be drained outside the plant

  7. Effects of silicate weathering on water chemistry in forested, upland, felsic terrane of the USA

    International Nuclear Information System (INIS)

    Stauffer, R.E.; Wittchen, B.D.

    1991-01-01

    The authors use data from the US EPA National Surface Water Survey (NSWS), the USGS Bench-Mark Station monitoring program, and the National Acid Deposition Program (NADP) to evaluate the role of weathering in supplying base cations to surface waters in forested, upland, felsic terrane of the northeastern, northcentral, and northwestern (Idaho batholith) US. Multivariate regression reveals differential effects of discharge on individual base cations and silica, but no secular trend in the Ca/Na denudation rate over 24 yr (1965-1988) for the Wild River catchment in the White Mountains. Because the turn-over time for Na in the soil-exchange complex is only ca. 1.5 yr, the long-term behavior of the ratios Ca/Na and Si/Na in waters leaving this catchment indicates that weathering is compensating for base cation export. In every subregion, Ca and Mg concentrations in lakes are statistically linked to nonmarine Na, but the median Ca/Na ratio is greater than the ratio in local plagioclase. The authors attribute this inequality to nonstoichiometric weathering of calcium in juvenile (formerly glaciated) terrane, not to leaching of exchangeable cations by So 4 because intraregional and cross-regional statistical analysis reveals no effect of atmospherically derived sulfate ion. The median base cation denudation rates (meq m -2 yr -1 ) for these American lake regions are: Maine granites (108); western Adirondack felsic gneiss (85); Vermilion batholith (42); Idaho batholith (52). The regional rates are high enough to compensate for present wet deposition of acidifying anions except in some vulnerable lake watersheds in the western Adirondacks

  8. Effects of activation of endocannabinoid system on myocardial metabolism

    Directory of Open Access Journals (Sweden)

    Agnieszka Polak

    2016-05-01

    Full Text Available Endocannabinoids exert their effect on the regulation of energy homeostasis via activation of specific receptors. They control food intake, secretion of insulin, lipids and glucose metabolism, lipid storage. Long chain fatty acids are the main myocardial energy substrate. However, the heart exerts enormous metabolic flexibility emphasized by its ability to utilzation not only fatty acids, but also glucose, lactate and ketone bodies. Endocannabinoids can directly act on the cardiomyocytes through the CB1 and CB2 receptors present in cardiomyocytes. It appears that direct activation of CB1 receptors promotes increased lipogenesis, pericardial steatosis and bioelectrical dysfunction of the heart. In contrast, stimulation of CB2 receptors exhibits cardioprotective properties, helping to maintain appropriate amount of ATP in cardiomyocytes. Furthermore, the effects of endocannabinoids at both the central nervous system and peripheral tissues, such as liver, pancreas, or adipose tissue, resulting indirectly in plasma availability of energy substrates and affects myocardial metabolism. To date, there is little evidence that describes effects of activation of the endocannabinoid system in the cardiovascular system under physiological conditions. In the present paper the impact of metabolic diseases, i. e. obesity and diabetes, as well as the cardiovascular diseases - hypertension, myocardial ischemia and myocardial infarction on the deregulation of the endocannabinoid system and its effect on the metabolism are described.

  9. The Effects of the Types of Milk (Cow, Goat, Soya and Enzymes (Rennet, Papain, Bromelain Toward Cheddar Cheese Production

    Directory of Open Access Journals (Sweden)

    Ariestya Arlene

    2015-04-01

    Full Text Available The objectives of this research are to study the effects of different types of milk and enzymes toward the yield and quality (moisture, ash, protein, fat content, and texture of cheddar cheese and the interaction between those two variables during the process. The types of milk are cow, goat, and soya milk, while the types of enzymes are rennet, papain, and bromelain enzymes. Regarding the procedure, the milk is first pasteurized before CaCl2 and Lactobacillus lactis that acts as the acidifier starter as much as 0.2% (w/v and 0.5% of the milk volume are added respectively. The amount of enzyme added is appropriate for the determination of enzyme dose. The curd is separated from the whey and then 2.5 grams of salt is added to 100 grams of curd. Afterwards, the curd is pressed until the water content decreases (cheese, then ripened for 1 month. The analyses conducted are moisture, ash, protein, fat content, and texture (hardness. The conclusion is the goat milk and the rennet enzyme are the suitable raw material for cheddar cheese production. Furthermore, different types of milk and enzymes affect the yield. However, there is no interaction between the types of milk and enzymes to the yield.

  10. On the Temporal Delay Assumption and the Impact of Non-Linguistic Context Effects

    Science.gov (United States)

    Brysbaert, Marc; van Wijnendaele, Ilse; Duyck, Wouter

    2002-01-01

    It is not easy to comment on Dijkstra and Van Heuven's model because there are many more aspects we agree with than aspects we feel uncomfortable about. Indeed, the BIA model has played an enormous role in showing us how bilingual visual word recognition can be achieved without recurrence to the intuitively appealing--but wrong--ideas of separate,…

  11. Nonstarter Lactobacillus strains as adjunct cultures for cheese making: in vitro characterization and performance in two model cheeses.

    Science.gov (United States)

    Briggiler-Marcó, M; Capra, M L; Quiberoni, A; Vinderola, G; Reinheimer, J A; Hynes, E

    2007-10-01

    Nonstarter lactic acid bacteria are the main uncontrolled factor in today's industrial cheese making and may be the cause of quality inconsistencies and defects in cheeses. In this context, adjunct cultures of selected lactobacilli from nonstarter lactic acid bacteria origin appear as the best alternative to indirectly control cheese biota. The objective of the present work was to study the technological properties of Lactobacillus strains isolated from cheese by in vitro and in situ assays. Milk acidification kinetics and proteolytic and acidifying activities were assessed, and peptide mapping of trichloroacetic acid 8% soluble fraction of milk cultures was performed by liquid chromatography. In addition, the tolerance to salts (NaCl and KCl) and the phage-resistance were investigated. Four strains were selected for testing as adjunct cultures in cheese making experiments at pilot plant scale. In in vitro assays, most strains acidified milk slowly and showed weak to moderate proteolytic activity. Fast strains decreased milk pH to 4.5 in 8 h, and continued acidification to 3.5 in 12 h or more. This group consisted mostly of Lactobacillus plantarum and Lactobacillus rhamnosus strains. Approximately one-third of the slow strains, which comprised mainly Lactobacillus casei, Lactobacillus fermentum, and Lactobacillus curvatus, were capable to grow when milk was supplemented with glucose and casein hydrolysate. Peptide maps were similar to those of lactic acid bacteria considered to have a moderate proteolytic activity. Most strains showed salt tolerance and resistance to specific phages. The Lactobacillus strains selected as adjunct cultures for cheese making experiments reached 10(8) cfu/g in soft cheeses at 7 d of ripening, whereas they reached 10(9) cfu/g in semihard cheeses after 15 d of ripening. In both cheese varieties, the adjunct culture population remained at high counts during all ripening, in some cases overcoming or equaling primary starter. Overall

  12. Response of marine and freshwater algae to nitric acid and elevated carbon dioxide levels simulating environmental effects of bolide impact

    Science.gov (United States)

    Boston, P. J.

    1988-01-01

    One of the intriguing facets of the Cretaceous-Tertiary extinction is the apparently selective pattern of mortality amongst taxa. Some groups of organisms were severely affected and some remained relatively unscathed as they went through the K/T boundary. While there is argument concerning the exact interpretation of the fossil record, one of the best documented extinctions at the Cretaceous-Tertiary boundary is that of the calcareous nannoplankton. These organisms include coccolithic algae and foraminiferans. Attempts to explain their decline at the K/T boundary center around chemistry which could affect their calcium carbonate shells while leaving their silica-shelled cousins less affected or unaffected. Two environmental consequences of an extraterrestrial body impact which were suggested are the production of large quantities of nitrogen oxides generated by the shock heating of the atmosphere and the possible rise in CO2 from the dissolution of CaCO3 shells. Both of these phenomena would acidify the upper layers of the oceans and bodies of freshwater not otherwise buffered. The effects of nitric acid, carbon dioxide, or both factors on the growth and reproduction of calcareous marine coccoliths and non-calcareous marine and freshwater species of algae were considered. These experiments demonstrate that nitric acid and carbon dioxide have significant effects on important aspects of the physiology and reproduction of modern algae representative of extinct taxa thought to have suffered significant declines at the Cretaceous-Tertiary boundary. Furthermore, calcareous species showed more marked effects than siliceous species and marine species tested were more sensitive than freshwater species.

  13. Comparative effects of indium/ytterbium doping on, mechanical and gas-sensitivity-related morphological, properties of sprayed ZnO compounds

    International Nuclear Information System (INIS)

    Boukhachem, A.; Fridjine, S.; Amlouk, A.; Boubaker, K.; Bouhafs, M.; Amlouk, M.

    2010-01-01

    In this study, conducting and transparent indium-doped zinc oxide (ZnO) thin films have been deposited on glass substrates by the micro-spray technique. First, zinc oxide layers were obtained by spaying a solution of propanol and zinc acetate in acidified medium. Alternatively, some of the obtained films were doped with indium (In) at the molar rates of: 1%, 2% and 3%. In addition to the classical structural investigated using XRD, AFM and SEM techniques, microhardness Vickers (Hv) measurements have been carried out along with comparative morphological prospecting. The specific gases sensitivity-related surface morphology of the doped ZnO compounds was favorably different from that of the non-doped ones, and showed a thin overlay structure. Results were compared to those recorded for similar ytterbium-doped material.

  14. Tålegrænser for luftforurening: Anvendelse i strategisk miljøplanlægning

    DEFF Research Database (Denmark)

    Bastrup-Birk, A.; Tybirk, K.; Wier, M.

    The report describes and discusses the principle of Critical Loads and Critical Levels in relation to strategic environmental planning. The DPSIR-chain explanatory variables are used as a framework for the process if integrating economics, chemistry and ecology in the field of acidifying...

  15. Plasma membrane H+-ATPase-dependent citrate exudation from cluster roots of phosphate-deficient white lupin

    DEFF Research Database (Denmark)

    Tomasi, Nicola; Kretzschmar, Tobias; Espen, Luca

    2009-01-01

    ABSTRACT White lupin (Lupinus albus L.) is able to grow on soils with sparingly available phosphate (P) by producing specialized structures called cluster roots.To mobilize sparingly soluble P forms in soils, cluster roots release substantial amounts of carboxylates and concomitantly acidify the ...

  16. Evaluation of the Efficacy, Safety, and Tolerability of 3 Dose Regimens of Topical Sodium Nitrite With Citric Acid in Patients With Anogenital Warts A Randomized Clinical Trial

    NARCIS (Netherlands)

    Ormerod, Anthony D.; van Voorst Vader, Pieter C.; Majewski, Slovomir; Vanscheidt, Wolfgang; Benjamin, Nigel; van der Meijden, Willem

    IMPORTANCE Anogenital warts are a common disorder associated with significant physical and mental distress and a substantial cause of health care costs. OBJECTIVE To assess the efficacy of the topical application of nitric oxide delivered using acidified nitrite. DESIGN, SETTING, AND PARTICIPANTS A

  17. Electrokinetic remediation of copper mine tailings

    DEFF Research Database (Denmark)

    Hansen, Henrik K.; Rojo, Adrián; Ottosen, Lisbeth M.

    2007-01-01

    in sulphuric acidified tailings) without bipolar electrodes to 42% when bipolar electrodes were implemented. Furthermore, the results showed that in this system sulphuric acid addition prior to remediation was better than citric acid addition. In addition, applying a too strong electric field (even...

  18. Illustrating the benefit of using hourly monitoring data on secondary inorganic aerosol and its precursors for model evaluation

    NARCIS (Netherlands)

    Schaap, M.; Otjes, R.P.; Weijers, E.P.

    2010-01-01

    Secondary inorganic aerosol, most notably ammonium nitrate and ammonium sulphate, is an important contributor to ambient particulate mass and provides a means for long range transport of acidifying components. The modelling of the formation and fate of these components is challenging. Especially,

  19. Illustrating the benefit of using hourly monitoring data on secondary inorganic aerosol and its precursors for model evaluation

    NARCIS (Netherlands)

    Schaap, M.; Otjes, R.P.; Weijers, E.P.

    2011-01-01

    Secondary inorganic aerosol, most notably ammonium nitrate and ammonium sulphate, is an important contributor to ambient particulate mass and provides a means for long range transport of acidifying components. The modelling of the formation and fate of these components is challenging. Especially,

  20. Simultaneous determination of acidic 3,4-dihydroxyphenylalanine metabolites and 5-hydroxyindole-3-acetic acid in urine by high-performance liquid chromatography

    NARCIS (Netherlands)

    Stroomer, A. E.; Overmars, H.; Abeling, N. G.; van Gennip, A. H.

    1990-01-01

    We describe a simple and rapid quantitative method for the simultaneous determination of 3,4-dihydroxyphenylalanine acid metabolites and 5-hydroxyindole-3-acetic acid. After solvent extraction from acidified urine, the acids are analyzed by reversed-phase high-performance liquid chromatography. For

  1. Synthesis of novel precursors of Pfitzinger reaction: A facile one-pot ...

    Indian Academy of Sciences (India)

    strategy to the synthesis of quinoline carboxylic acid derivatives of ... solution of sodium nitrite (0.7g in 2ml water) while ... sodium acetate trihydrate (1.80 g) in methanol (10 ml) ... the aqueous layer was acidified with acetic acid till neu-.

  2. Phosphorus fractionation and crop performance on an alfisol ...

    African Journals Online (AJOL)

    SERVER

    2007-08-20

    Aug 20, 2007 ... acidified ammonium persulphate oxidation (Environmental Protection. Agency, 1971), and analyzed for total P. Inorganic phosphate in the extracts was determined colorimetrically with the molybdate-ascorbic acid procedure (Murphy and Riley, 1962). Organic P was calculated as the difference between ...

  3. Untitled

    Indian Academy of Sciences (India)

    acetyl-4-hydroxycoumarin (2 g) was taken in 30 ml of 15% aqueous Sodium hydroxide and was refluxed for 2 hr. The Solution was cooled and acidified. The compound separatei was filtered and was crys- tallised from methanol, mp, mmp with ...

  4. Characterization of a Stabilized Form of Microplasmin for the Induction of Posterior Vitreous Detachment

    NARCIS (Netherlands)

    Gad Elkareem, Ashraf M.; Willekens, Ben; Vanhove, Marc; Noppen, Bernard; Stassen, Jean Marie; de Smet, Marc D.

    2010-01-01

    Purpose: To investigate the stability and safety of a diluted acidified form of microplasmin and its ability to induce a posterior vitreous detachment (PVD) following intravitreal injection in postmortem porcine eyes. Methods: Microplasmin diluted in normal saline (NS) and balanced salt solution

  5. Power system for tokamak fusion experiments. Motor generator with flywheel effect

    International Nuclear Information System (INIS)

    Miyachi, Kengo

    1997-01-01

    JT-60 requires an enormous electric power pulse about 1,300 MVA periodically for its plasma initiation, containment and heating. JT-60 could not receive all electric power from a commercial line for plasma experiment except about 160 MVA because the 275 kV commercial line has some limitations. Therefore JT-60 needs huge electric power sources. The power supply system of JT-60 has 3 motor generators (MG). The total capacity of MG is 1,115 MVA that consists of a toroidal MG (TMG), poloidal MG (PMG) and Heating power supply MG (HMG), and each MG has a huge flywheel effect. For example, TMG has a 4.02 GJ energy yield that consists of 6 disk flywheel. The total weight of flywheel of TMG is 650 ton. This report describes the structure, operating system, and maintenance history of 3 types of MG. (author)

  6. A review and rationale for studying the cardiovascular effects of drinking water arsenic in women of reproductive age

    International Nuclear Information System (INIS)

    Kwok, Richard K.

    2007-01-01

    Drinking water arsenic has been shown to be associated with a host of adverse health outcomes at exposure levels > 300 μg of As/L. However, the results are not consistent at exposures below this level. We have reviewed selected articles that examine the effects of drinking water arsenic on cardiovascular outcomes and present a rationale for studying these effects on women of reproductive age, and also over the course of pregnancy when they would potentially be more susceptible to adverse cardiovascular and reproductive outcomes. It is only recently that reproductive effects have been linked to drinking water arsenic. However, there is a paucity of information about the cardiovascular effects of drinking water arsenic on women of reproductive age. Under the cardiovascular challenge of pregnancy, we hypothesize that women with a slightly elevated exposure to drinking water arsenic may exhibit adverse cardiovascular outcomes at higher rates than in the general population. Studying sensitive clinical and sub-clinical indicators of disease in susceptible sub-populations may yield important information about the potentially enormous burden of disease related to low-level drinking water arsenic exposure

  7. Interactions between milk protein ingredients and other milk components during processing

    DEFF Research Database (Denmark)

    Liu, Guanchen

    research in our group shown that, both MWP and NWP can give a higher viscosity and denser microstructure compared to WPC when used as fat replacer in low-fat yoghurt. In the thesis, we investigated how these two types of commercial whey protein particles interact with other milk components and how...... these interactions affect final acidified milk products. By detecting the properties of the whey protein aggregates, MWP and NWP showed low native whey protein content, low free thiol content and high surface hydrophobicity and were relatively stable at high temperature in the 5 % pure dispersions. When MWP and NWP...... were added to non-fat milk model systems (5% protein in total) and processed into chemically (glucono-delta-lactone) acidified milk gels, the formation of disulfide-linked structures was closely related to the increased particle size of heated milk model systems and the rheological behavior...

  8. Large-scale feasibility of organic acids as a permanent preharvest intervention in drinking water of broilers and their effect on foodborne Campylobacter spp. before processing.

    Science.gov (United States)

    Jansen, W; Reich, F; Klein, G

    2014-06-01

    Evaluating the effect of a commercially available organic acid water additive in conventional broiler production on Campylobacter spp. The organic acid water additive was added to the drinking water from chick housing to catching in three consecutive rearing cycles. The broiler performance data were evaluated, and the load of thermophilic Campylobacter spp. was analysed in water, feed and the environment as well as determined in caecum content and on carcasses at the abattoir according to ISO 10272:1.2-2002. The results indicated that permanent application of acidified drinking water did not have detrimental effects on production parameters or animal welfare. The quantitative results obtained at slaughter were ambiguous, but suggested a reduced carriage of Campylobacter spp. by the flock and in caecum content. Such reduction did not result in lower Campylobacter carriage of the carcasses after slaughter. Organic acids in drinking water of broilers can partly reduce the caecal Campylobacter spp. load, but this did not reduce carcass contamination. Broiler meat is a major source of foodborne campylobacteriosis. The public health would considerably benefit from controlling Campylobacter in the food chain. The addition of organic acid to drinking water of broilers can potentially lower the caecal carriage in primary production. However, in this field trial, a commercial product failed to have an impact on the bacterial load after slaughter. © 2014 The Society for Applied Microbiology.

  9. Elimination of salmonella from animal glandular products.

    Science.gov (United States)

    De Fiebre, C W; Burck, K T; Feldman, D

    1969-03-01

    Methods for the elimination of salmonellae from selected powdered pharmaceuticals of animal glandular origin were studied. Terminal heat treatment under carefully controlled conditions was effective for pancreatin-a powder containing proteolytic, amylolytic, and lipolytic enzymes prepared from hog pancreas glands. Use of this method resulted in a significant reduction in the number of salmonella-positive batches and also reduced the testing procedures required to confirm the absence of viable salmonellae among the majority of samples tested. Powders such as stomach substance and thyroid, in which the biological activity is not enzyme in nature, were treated successfully with acidified organic solvents. Other methods were investigated but were not suitable because of a deleterious effect on the biological activity or physical properties of the product or an inability to effect salmonella elimination.

  10. Elimination of Salmonellae from Animal Glandular Products

    Science.gov (United States)

    De Fiebre, Conrad W.; Burck, Kenneth T.; Feldman, David

    1969-01-01

    Methods for the elimination of salmonellae from selected powdered pharmaceuticals of animal glandular origin were studied. Terminal heat treatment under carefully controlled conditions was effective for pancreatin—a powder containing proteolytic, amylolytic, and lipolytic enzymes prepared from hog pancreas glands. Use of this method resulted in a significant reduction in the number of salmonella-positive batches and also reduced the testing procedures required to confirm the absence of viable salmonellae among the majority of samples tested. Powders such as stomach substance and thyroid, in which the biological activity is not enzyme in nature, were treated successfully with acidified organic solvents. Other methods were investigated but were not suitable because of a deleterious effect on the biological activity or physical properties of the product or an inability to effect salmonella elimination. PMID:5780395

  11. Predicting nitrogen and acidity effects on long-term dynamics of dissolved organic matter

    International Nuclear Information System (INIS)

    Rowe, E.C.; Tipping, E.; Posch, M.; Oulehle, F.; Cooper, D.M.; Jones, T.G.; Burden, A.; Hall, J.; Evans, C.D.

    2014-01-01

    Increases in dissolved organic carbon (DOC) fluxes may relate to changes in sulphur and nitrogen pollution. We integrated existing models of vegetation growth and soil organic matter turnover, acid–base dynamics, and organic matter mobility, to form the ‘MADOC’ model. After calibrating parameters governing interactions between pH and DOC dissolution using control treatments on two field experiments, MADOC reproduced responses of pH and DOC to additions of acidifying and alkalising solutions. Long-term trends in a range of acid waters were also reproduced. The model suggests that the sustained nature of observed DOC increases can best be explained by a continuously replenishing potentially-dissolved carbon pool, rather than dissolution of a large accumulated store. The simulations informed the development of hypotheses that: DOC increase is related to plant productivity increase as well as to pH change; DOC increases due to nitrogen pollution will become evident, and be sustained, after soil pH has stabilised. -- Highlights: • A model of dissolved organic carbon (DOC) was developed by integrating simple models • MADOC simulates effects of sulphur and nitrogen deposition and interactions with pH. • Responses of DOC and pH to experimental acidification and alkalisation were reproduced. • The persistence of DOC increases will depend on continued supply of potential DOC. • DOC fluxes are likely determined by plant productivity as well as soil solution pH. -- Effects of changes in sulphur and nitrogen pollution on dissolved organic carbon fluxes are predicted by simulating soil organic matter cycling, the release of potentially-dissolved carbon, and interactions with soil pH

  12. Effects of ocean acidification on juvenile red king crab (Paralithodes camtschaticus and Tanner crab (Chionoecetes bairdi growth, condition, calcification, and survival.

    Directory of Open Access Journals (Sweden)

    William Christopher Long

    Full Text Available Ocean acidification, a decrease in the pH in marine waters associated with rising atmospheric CO2 levels, is a serious threat to marine ecosystems. In this paper, we determine the effects of long-term exposure to near-future levels of ocean acidification on the growth, condition, calcification, and survival of juvenile red king crabs, Paralithodes camtschaticus, and Tanner crabs, Chionoecetes bairdi. Juveniles were reared in individual containers for nearly 200 days in flowing control (pH 8.0, pH 7.8, and pH 7.5 seawater at ambient temperatures (range 4.4-11.9 °C. In both species, survival decreased with pH, with 100% mortality of red king crabs occurring after 95 days in pH 7.5 water. Though the morphology of neither species was affected by acidification, both species grew slower in acidified water. At the end of the experiment, calcium concentration was measured in each crab and the dry mass and condition index of each crab were determined. Ocean acidification did not affect the calcium content of red king crab but did decrease the condition index, while it had the opposite effect on Tanner crabs, decreasing calcium content but leaving the condition index unchanged. This suggests that red king crab may be able to maintain calcification rates, but at a high energetic cost. The decrease in survival and growth of each species is likely to have a serious negative effect on their populations in the absence of evolutionary adaptation or acclimatization over the coming decades.

  13. Sensory Characteristics and Comparison of Commercial Plain Yogurts and 2 New Production Sample Options.

    Science.gov (United States)

    Brown, Marissa D; Chambers, Delores H

    2015-12-01

    This research determined the sensory characteristics of currently available plain yogurts available in U.S. supermarkets and examined how 3 "more sustainable" prototypes compared. The prototypes, nonfat set-style yogurts pre-acidified after pasteurization with lemon juice or citric acid at 80 ppm to pH 6.2, had shorter fermentation times than the lab-made control. These reduced fermentation times could result in energy reductions and potentially substantiate a "sustainable" marketing claim, a concept gaining traction with consumers. Twenty-six commercial yogurts, varying in percent milk fat, milk source (organic or conventional), and processing (set-style, stirred, or strained/Greek-style), were also included. Using descriptive sensory analysis, a 6-person highly trained panel scored the intensity of 25 flavor and 10 texture attributes on a 15-point scale. Three replications were carried out, and all samples were tested at least 10 d prior to the end of their shelf-lives. The samples differed for 19 flavor and all 10 texture attributes. Cluster analysis indicated approximately 7 flavor and 5 texture clusters. The prototype pre-acidified with lemon juice was similar to category leaders nonfat yogurt varieties. The prototype pre-acidified with citric acid was similar in texture but was less sour. Although no legal definitions exist for "sustainable," the prototypes' sensory characteristics are comparable to those of popular yogurts indicating potential market viability. This research also demonstrates potential for making yogurt that is in line with growing consumer expectations for sustainability. Despite the current diversity, several combinations of flavor and texture were not represented. © 2015 Institute of Food Technologists®

  14. Role of hydrogen ions in standard and activation heap leaching of gold

    Science.gov (United States)

    Rubtsov, YuI

    2017-02-01

    The role of hydrogen ions in activation heap leaching of gold from rebellious ore has been studied, which has allowed enhancing gold recovery. The author puts forward a gold leaching circuit with the use of activated oxygen-saturated solutions acidified to pH = 6-9.

  15. Rapid and simple clean-up and derivatizaton procedure for the gas chromatographic determination of acidic drugs in plasma

    NARCIS (Netherlands)

    Roseboom, H.; Hulshoff, A.

    1979-01-01

    A rapid and simple clean-up and derivatization procedure that can be generally applied to the gas chromatographie (GC) determination of acidic drugs of various chemical and therapeutic classes is described. The drugs are extracted from acidified plasma with chloroform containing 5% of isopropanol,

  16. The effect of alum addition on microbial communities in poultry litter.

    Science.gov (United States)

    Rothrock, M J; Cook, K L; Warren, J G; Sistani, K

    2008-08-01

    Alum [Al(2)(SO(4))(3).14H(2)O] is a common poultry litter amendment used to decrease water-soluble phosphorus or reduce ammonia volatilization, or both. Although the physiochemical effects of alum addition have been well researched, little attention has been given to the poultry litter microbial communities. The goal of this study was to use molecular biological methods [denaturing gradient gel electrophoresis (DGGE), community cloning, and quantitative real-time PCR] to characterize general, group-specific and pathogenic microbial communities in alum (10% wt/wt) and non-alum-treated litter. According to quantitative real-time PCR analyses, alum addition to the poultry litter resulted in significant reductions in both Campylobacter jejuni and Escherichia coli concentrations by the end of the first month of the experiment (3 log and 2 log, respectively). The concentrations of Salmonella spp. were below detection (Eubacterium and low %GC gram-positive groups in the alum-treated litters by the end of the first month, with no bands detectable for either group after 8 wk of incubation. Conversely, minimal effects of alum addition were observed in the Actinomycetes community. The most significant shift in the microbial community (based on DGGE analyses) occurred in the fungal population, with a large increase in diversity and abundance within 1 mo of alum addition (1 dominant band on d 0 to 9 dominant bands at 4 wk). Specifically, the incidence of Aspergillus spp. increased from 0 to 50% of the sequences in fungal clone libraries (n = 80) over the course of the experiment. This suggests that the addition of alum to poultry litter potentially shifts the microbial populations from bacterially dominated to dominated by fungi. The ramifications of this shift in dominance are still unknown, and future work will be aimed at characterizing these fungi and elucidating their role in the acidified litter environment.

  17. Application of exogenous ethylene inhibits postharvest peel browning of ‘Huangguan’ pear

    Science.gov (United States)

    Peel browning disorder has an enormous impact on the exterior quality of ‘Huangguan’ pear whereas the underlying mechanism is still unclear. In this study, the effect of exogenous ethylene on peel browning of pear fruits stored at 0' was evaluated. Results showed that ethylene effectively inhibited ...

  18. Ultrasonic monitoring of yoghurt formation by using AT-cut quartz: lighting of casein micelles interactions process during the acidification.

    Science.gov (United States)

    Ould-Ehssein, C; Serfaty, S; Griesmar, P; Le Huérou, J-Y; Caplain, E; Martinez, L; Wilkie-Chancellier, N; Gindre, M

    2006-12-22

    The behavior of weak gels during their formation singularly attracts attention of dairy products factories. In our study we investigate acidified pre-heated milk gels formation that are fairly often used to product yoghurts. The gel formation requires a tight control of the first step of micelles modification process and the kinetics reaction parameters. The most current rheological parameters used to achieve the monitoring are the storage G' and the loss G'' shear moduli and the gelation time. The study of these parameters is commonly performed at very low frequencies (1 Hz). Our technique uses a 6 MHz AT-cut quartz crystal immersed in an acidified milk solution kept at a constant temperature. This method is singularly effective to ensure a complete and a reliable follow-up of the viscoelastic parameters of casein gels. A suitable new model enables a complete follow-up of the micelles evolution from the viscoelastic properties. The experimental results of the G' and G'' moduli versus temperature and versus glucono-delta-lactone (GDL) added to milk are analyzed. In order to understand the micelles modifications, an analysis of the viscoelastic evolution try to explain the validity of the various models of micelles modification. In addition a new accurate kinetics characteristic time is proposed. This time corresponds to the moment for which the elastic effect of material becomes significant. From the kinetics study of casein gels at various temperatures, the Arrhenius relationship and a modified Flory-Stockmayer relationship give us access to the activation energy. By using the proposed technique and the suitable models developed, the structure thus quality of dairy products may be better controlled.

  19. Microbial mineralization of organic nitrogen forms in poultry litters.

    Science.gov (United States)

    Rothrock, Michael J; Cook, Kimberly L; Warren, Jason G; Eiteman, Mark A; Sistani, Karamat

    2010-01-01

    Ammonia volatilization from the mineralization of uric acid and urea has a major impact on the poultry industry and the environment. Dry acids are commonly used to reduce ammonia emissions from poultry houses; however, little is known about how acidification affects the litter biologically. The goal of this laboratory incubation was to compare the microbiological and physiochemical effects of dry acid amendments (Al+Clear, Poultry Litter Treatment, Poultry Guard) on poultry litter to an untreated control litter and to specifically correlate uric acid and urea contents of these litters to the microbes responsible for their mineralization. Although all three acidifiers eventually produced similar effects within the litter, there was at least a 2-wk delay in the microbiological responses using Poultry Litter Treatment. Acidification of the poultry litter resulted in >3 log increases in total fungal concentrations, with both uricolytic (uric acid degrading) and ureolytic (urea degrading) fungi increasing by >2 logs within the first 2 to 4 wk of the incubation. Conversely, total, uricolytic, and ureolytic bacterial populations all significantly declined during this same time period. While uric acid and urea mineralization occurred within the first 2 wk in the untreated control litter, acidification resulted in delayed mineralization events for both uric acid and urea (2 and 4 wk delay, respectively) once fungal cell concentrations exceeded a threshold level. Therefore, fungi, and especially uricolytic fungi, appear to have a vital role in the mineralization of organic N in low-pH, high-N environments, and the activity of these fungi should be considered in best management practices to reduce ammonia volatilization from acidified poultry litter.

  20. Effect of a generalized particle momentum distribution on plasma nuclear fusion rates

    International Nuclear Information System (INIS)

    Kim, Yeong E.; Zubarev, Alexander L.

    2006-01-01

    We investigate the effect of a generalized particle momentum distribution derived by Galitskii and Yakimets (GY) on nuclear reaction rates in plasma. We derive an approximate semi-analytical formula for nuclear fusion reaction rate between nuclei in a plasma (quantum plasma nuclear fusion; or QPNF). The QPNF formula is applied to calculate deuteron-deuteron fusion rate in a plasma, and the results are compared with the results calculated with the conventional Maxwell-Boltzmann velocity distribution. As an application, we investigate the deuteron-deuteron fusion rate for mobile deuterons in a deuterated metal/alloy. The calculated deuteron-deuteron fusion rates at low energies are enormously enhanced due to the modified tail of the GY's generalized momentum distribution. Our preliminary estimates indicate also that the deuteron-lithium (D+Li) fusion rate and the proton-lithium (p+Li) fusion rate in a metal/alloy at ambient temperatures are also substantially enhanced. (author)

  1. The global security perspective on the effects of executive cognitive function on complex behavioral screening intervention and HIV/AIDS.

    Science.gov (United States)

    Kim, Suk-Hee

    2010-11-01

    The purpose of this quantitative study is to understand the global security perspective on the effects of executive cognitive function (ECF) on Complex Behavioral Screening Intervention and HIV/AIDS. The HIV/AIDS pandemic is as much a social, political, economic, and cultural problem as a biomedical one. HIV/AIDS is associated centrally with the collapse not just of communities and families but potentially of states, with some of the largest public health interventions ever and enormous questions about governance, a huge population of orphans, and deep questions about intergenerational relations and cultural transmission. This study also is to develop a screening instrument that improves quality of life for individuals with executive cognitive impairments and behavior problems in our communities and the global society.

  2. Soil and vegetation changes after clear-felling coniferous forests: effects of varying removal of logging residues

    International Nuclear Information System (INIS)

    Olsson, Bengt.

    1995-01-01

    Effects of the intensity of logging residue harvesting on soil nutrient status and ground vegetation cover were examined over a 16-year period in two series of field experiments in Sweden. Short-term effects of slash harvesting and stump removal on soil water chemistry were studied after clear-felling a Norway spruce (Picea abies (L.) Karst.) stand in SW Sweden. Soil water concentrations of NH4 + , and NO 3 - and K + were lower shortly after whole-tree harvesting (i.e. stem and slash harvesting) than shortly after conventional stem-only harvesting or complete tree harvesting (i.e. stem, slash and stump removal). However, 5 years later there were no longer differences in nutrient concentrations detected between treatments, and nutrient levels approached those normally found in drainage water from forest land. Similar studies focussed on long-term (16 years) effects were conducted on four coniferous forest sites in Sweden, two in north and the other two in the south. In each region one site was situated in a pure Scots pine stand (Pinus sylvestris L.) and the other in a pure Norway spruce stand. In general, the intensity of slash harvesting had no effect on the total pools of nitrogen or carbon in the soil. Furthermore, this study showed experimentally that the harvesting of logging residues results in long-term soil acidification and depletions of exchangeable base cations, manganese and zinc pools, which lead in turn to a reduction in base saturation levels. A major implication for practical forestry was that guidelines and recommendations concerning the large-scale utilization of logging residues should be based more on the nutritional and soil acidifying consequences of this practice than on its potential effect on soil organic matter storage. It would also be possible to mitigate the detrimental effects that slash harvesting has on site conditions by applying wood-ash or other nutrients in inorganic form. 53 refs, 4 figs, 4 tabs

  3. Soil and vegetation changes after clear-felling coniferous forests: effects of varying removal of logging residues

    Energy Technology Data Exchange (ETDEWEB)

    Olsson, Bengt

    1995-11-01

    Effects of the intensity of logging residue harvesting on soil nutrient status and ground vegetation cover were examined over a 16-year period in two series of field experiments in Sweden. Short-term effects of slash harvesting and stump removal on soil water chemistry were studied after clear-felling a Norway spruce (Picea abies (L.) Karst.) stand in SW Sweden. Soil water concentrations of NH4{sup +}, and NO{sub 3}{sup -} and K{sup +} were lower shortly after whole-tree harvesting (i.e. stem and slash harvesting) than shortly after conventional stem-only harvesting or complete tree harvesting (i.e. stem, slash and stump removal). However, 5 years later there were no longer differences in nutrient concentrations detected between treatments, and nutrient levels approached those normally found in drainage water from forest land. Similar studies focussed on long-term (16 years) effects were conducted on four coniferous forest sites in Sweden, two in north and the other two in the south. In each region one site was situated in a pure Scots pine stand (Pinus sylvestris L.) and the other in a pure Norway spruce stand. In general, the intensity of slash harvesting had no effect on the total pools of nitrogen or carbon in the soil. Furthermore, this study showed experimentally that the harvesting of logging residues results in long-term soil acidification and depletions of exchangeable base cations, manganese and zinc pools, which lead in turn to a reduction in base saturation levels. A major implication for practical forestry was that guidelines and recommendations concerning the large-scale utilization of logging residues should be based more on the nutritional and soil acidifying consequences of this practice than on its potential effect on soil organic matter storage. It would also be possible to mitigate the detrimental effects that slash harvesting has on site conditions by applying wood-ash or other nutrients in inorganic form. 53 refs, 4 figs, 4 tabs

  4. Review of natural products with hepatoprotective effects.

    Science.gov (United States)

    Madrigal-Santillán, Eduardo; Madrigal-Bujaidar, Eduardo; Álvarez-González, Isela; Sumaya-Martínez, María Teresa; Gutiérrez-Salinas, José; Bautista, Mirandeli; Morales-González, Ángel; García-Luna y González-Rubio, Manuel; Aguilar-Faisal, J Leopoldo; Morales-González, José A

    2014-10-28

    The liver is one of the most important organs in the body, performing a fundamental role in the regulation of diverse processes, among which the metabolism, secretion, storage, and detoxification of endogenous and exogenous substances are prominent. Due to these functions, hepatic diseases continue to be among the main threats to public health, and they remain problems throughout the world. Despite enormous advances in modern medicine, there are no completely effective drugs that stimulate hepatic function, that offer complete protection of the organ, or that help to regenerate hepatic cells. Thus, it is necessary to identify pharmaceutical alternatives for the treatment of liver diseases, with the aim of these alternatives being more effective and less toxic. The use of some plants and the consumption of different fruits have played basic roles in human health care, and diverse scientific investigations have indicated that, in those plants and fruits so identified, their beneficial effects can be attributed to the presence of chemical compounds that are called phytochemicals. The present review had as its objective the collecting of data based on research conducted into some fruits (grapefruit, cranberries, and grapes) and plants [cactus pear (nopal) and cactus pear fruit, chamomile, silymarin, and spirulina], which are consumed frequently by humans and which have demonstrated hepatoprotective capacity, as well as an analysis of a resin (propolis) and some phytochemicals extracted from fruits, plants, yeasts, and algae, which have been evaluated in different models of hepatotoxicity.

  5. Review of natural products with hepatoprotective effects

    Science.gov (United States)

    Madrigal-Santillán, Eduardo; Madrigal-Bujaidar, Eduardo; Álvarez-González, Isela; Sumaya-Martínez, María Teresa; Gutiérrez-Salinas, José; Bautista, Mirandeli; Morales-González, Ángel; García-Luna y González-Rubio, Manuel; Aguilar-Faisal, J Leopoldo; Morales-González, José A

    2014-01-01

    The liver is one of the most important organs in the body, performing a fundamental role in the regulation of diverse processes, among which the metabolism, secretion, storage, and detoxification of endogenous and exogenous substances are prominent. Due to these functions, hepatic diseases continue to be among the main threats to public health, and they remain problems throughout the world. Despite enormous advances in modern medicine, there are no completely effective drugs that stimulate hepatic function, that offer complete protection of the organ, or that help to regenerate hepatic cells. Thus, it is necessary to identify pharmaceutical alternatives for the treatment of liver diseases, with the aim of these alternatives being more effective and less toxic. The use of some plants and the consumption of different fruits have played basic roles in human health care, and diverse scientific investigations have indicated that, in those plants and fruits so identified, their beneficial effects can be attributed to the presence of chemical compounds that are called phytochemicals. The present review had as its objective the collecting of data based on research conducted into some fruits (grapefruit, cranberries, and grapes) and plants [cactus pear (nopal) and cactus pear fruit, chamomile, silymarin, and spirulina], which are consumed frequently by humans and which have demonstrated hepatoprotective capacity, as well as an analysis of a resin (propolis) and some phytochemicals extracted from fruits, plants, yeasts, and algae, which have been evaluated in different models of hepatotoxicity. PMID:25356040

  6. Comparison of CSTR and UASB reactor configuration for the treatment of sulfate rich wastewaters under acidifying conditions

    NARCIS (Netherlands)

    Lopes, S.I.C.; Dreissen, C.; Capela, M.I.; Lens, P.N.L.

    2008-01-01

    The effects of lowering the operational pH from 6 to 5 on mesophilic (30 degrees C) sulfate reduction during the acidification of sucrose at an organic loading rate of 5 gCOD(1(reactor)d)(-1) and at a COD/SO42- ratio of 4 were evaluated in a CSTR and in a UASB reactor. The HRT was 24 h and 10 h,

  7. Acid mine drainage arising from gold mining activity in Johannesburg, South Africa and environs

    International Nuclear Information System (INIS)

    Naicker, K.; Cukrowska, E.; McCarthy, T.S.

    2003-01-01

    Ground water within the mining district is heavily contaminated and acidified. - The Witwatersrand region of South Africa is famous for its gold production and a major conurbation, centred on Johannesburg, has developed as a result of mining activity. A study was undertaken of surface and ground water in a drainage system in this area. Soils were also analysed from a site within the mining district. This study revealed that the ground water within the mining district is heavily contaminated and acidified as a result of oxidation of pyrite (FeS 2 ) contained within mine tailings dumps, and has elevated concentrations of heavy metals. Where the water table is close to surface, the upper 20 cm of soil profiles are severely contaminated by heavy metals due to capillary rise and evaporation of the ground water. The polluted ground water is discharging into streams in the area and contributes up to 20% of stream discharge, causing a lowering of pH of the stream water. Much of the metal load is precipitated in the stream: Fe and Mn precipitate as a consequence of oxidation, while other heavy metals are being removed by co-precipitation. The oxidation of iron has created a redox buffer which controls the pH of the stream water. The rate of oxidation and of dilution is slow and the deleterious effect of the addition of contaminated water persists for more than 10 km beyond the source

  8. Environmental system analysis of tomato production in Ghana ...

    African Journals Online (AJOL)

    The aim of this study was to identify the most important sources of greenhouse gases, acidifying and eutrophying compounds associated with tomato production in Ghana and identify options to reduce the environmental impacts. Life Cycle Analysis (LCA) methodology was used in the analysis (Cradle to gate approach).

  9. Starter culture development for improving the flavour of Proosdij-type cheese

    NARCIS (Netherlands)

    Ayad, E.H.E.; Verheul, A.; Bruinenberg, P.; Wouters, J.T.M.; Smit, G.

    2003-01-01

    The use of the additional mesophilic strain B851, which has specific flavour forming abilities, was tested for improving the flavour development of a Proosdij-type cheese made with a combination of an acidifying mesophilic and an adjunct thermophilic culture. This strain was selected because of its

  10. Ongoing transients in carbonate compensation

    NARCIS (Netherlands)

    Boudreau, B.P.; Middelburg, J.J.; Hofmann, A.F.; Meysman, F.J.R.

    2010-01-01

    Uptake of anthropogenic CO2 is acidifying the oceans. Over the next 2000 years, this will modify the dissolution and preservation of sedimentary carbonate. By coupling new formulas for the positions of the calcite saturation horizon, zsat, the compensation depth, zcc, and the snowline, zsnow, to a

  11. Spring Snowmelt and Mercury Export from a Forested Catchment in the Czech Republic, Central Europe

    Czech Academy of Sciences Publication Activity Database

    Navrátil, Tomáš; Rohovec, Jan; Hojdová, Maria; Vach, Marek

    2011-01-01

    Roč. 86, č. 6 (2011), s. 670-675 ISSN 0007-4861 R&D Projects: GA ČR GP526/07/P170 Institutional research plan: CEZ:AV0Z30130516 Keywords : mercury * DOC * acidified * catchment * snowmelt Subject RIV: DD - Geochemistry Impact factor: 1.018, year: 2011

  12. Electrochemical acidification of milk by whey desalination

    NARCIS (Netherlands)

    Balster, J.H.; Punt, Ineke G.M.; Stamatialis, Dimitrios; Lammers, H.; Verver, A.B.; Wessling, Matthias

    2007-01-01

    We describe a process configuration for the electrochemical acidification of milk using the desalination function and the acid/base production function of a bipolar membrane process. First, the milk is acidified by the acid produced in the bipolar membrane stack. The precipitate is removed by a

  13. European wet deposition maps based on measurements

    NARCIS (Netherlands)

    Leeuwen EP van; Erisman JW; Draaijers GPJ; Potma CJM; Pul WAJ van; LLO

    1995-01-01

    To date, wet deposition maps on a European scale have been based on long-range transport model results. For most components wet deposition maps based on measurements are only available on national scales. Wet deposition maps of acidifying components and base cations based on measurements are needed

  14. Decreased EGFR mRNA expression in response to antipsoriatic ...

    African Journals Online (AJOL)

    Dithranol is enormously effective in the treatment of psoriasis; however its molecular mode of action should be further elucidated. Since epidermal growth factor receptor (EGFR) is involved in the pathogenesis of psoriasis, the objective of this study was to investigate the transcriptional effect of dithranol on EGFR gene ...

  15. Uranium concentrations in natural waters, South Park, Colorado

    International Nuclear Information System (INIS)

    Sharp, R.R. Jr.; Aamodt, P.L.

    1976-08-01

    During the summer of 1975, 464 water samples from 149 locations in South Park, Colorado, were taken for the Los Alamos Scientific Laboratory in order to test the field sampling and analytical methodologies proposed for the NURE Hydrogeochemical and Stream Sediment Reconnaissance for uranium in the Rocky Mountain states and Alaska. The study showed, in the South Park area, that the analytical results do not vary significantly between samples which were untreated, filtered and acidified, filtered only, or acidified only. Furthermore, the analytical methods of fluorometry and delayed-neutron counting, as developed at the LASL for the reconnaissance work, provide fast, adequately precise, and complementary procedures for analyzing a broad range of uranium in natural waters. The data generated using this methodology does appear to identify uraniferous areas, and when applied using sound geochemical, geological, and hydrological principles, should prove a valuable tool in reconnaissance surveying to delineate new districts or areas of interest for uranium exploration

  16. A mobile killing- and mincing unit represents a possible alternative in mass destruction of AIV infected poultry

    DEFF Research Database (Denmark)

    Jørgensen, Poul Henrik; Nielsen, Anne Ahlmann; Handberg, Kurt

    steps in the disposal process. At first, the hens are transported into a chamber where they are killed by CO2. The dead hens are subsequently transported on a conveyor belt to the disintegrating mincing device, and the produced pulp is accumulated in a container under constant mechanical stirring....... In order to prevent bacterial growth and putrefaction, the pulp is acidified to pH below 2.0. Finally, the pulp is transferred via closed pipes to a container on a lorry before transportation to its final destination as mink feed. Importantly, all steps in this process are strictly supervised and adjusted...... was added in the laboratory to freshly produced pulp, and the survival of infectious virus as well as presence of genome segments were monitored over a 24-hour period. Interestingly, H5N2 AIV was instantly inactivated in the acidified pulp, whereas AIV survival was documented for at least 24 hours...

  17. Fly ash as a sorbent for the removal of biologically resistant organic matter

    Energy Technology Data Exchange (ETDEWEB)

    Vandenbusch, M B; Sell, N J [Wisconsin Public Service Corporation, Green Bay, WI (USA)

    1992-02-01

    Six different fly ashes, in both an untreated and an acidified form, were studied with respect to their ability to remove colour and organic materials from a municipal waste treatment facility effluent. Colour, fluorescence, and chemical oxygen demand were used to monitor the removal. The apparent predominant mechanism varies with the pH and the chemical characteristics of the ash. These mechanisms include carbon sorption, calcium precipitation of tannins and humics, sorption on the fly ash surface by silica, alumina, and/or iron oxide and, in the acidified situation, coagulation of coloured colloids in the effluent dissolved from the fly ash. Some additional deleterious components are dissolved into the effluent during the treatment process. Many of these materials can, however, be removed by using a two-stage fly ash sorption process. Particularly of interest is the ability to remove over 90% of the boron from the effluent. 13 refs., 10 figs., 9 tabs.

  18. Intraspecific variations in responses to ocean acidification in two branching coral species.

    Science.gov (United States)

    Sekizawa, Ayami; Uechi, Hikaru; Iguchi, Akira; Nakamura, Takashi; Kumagai, Naoki H; Suzuki, Atsushi; Sakai, Kazuhiko; Nojiri, Yukihiro

    2017-09-15

    Ocean acidification is widely recognised to have a negative impact on marine calcifying organisms by reducing calcifications, but controversy remains over whether such organisms could cope with ocean acidification within a range of phenotypic plasticity and/or adapt to future acidifying ocean. We performed a laboratory rearing experiment using clonal fragments of the common branching corals Montipora digitata and Porites cylindrica under control and acidified seawater (lower pH) conditions (approximately 400 and 900μatm pCO 2 , respectively) and evaluated the intraspecific variations in their responses to ocean acidification. Intra- and interspecific variations in calcification and photosynthetic efficiency were evident according to both pCO 2 conditions and colony, indicating that responses to acidification may be individually variable at the colony level. Our results suggest that some corals may cope with ocean acidification within their present genotypic composition by adaptation through phenotypic plasticity, while others may be placed under selective pressures resulting in population alteration. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. A fermented meat model system for studies of microbial aroma formation

    DEFF Research Database (Denmark)

    Tjener, Karsten; Stahnke, Louise Heller; Andersen, L.

    2003-01-01

    A fermented meat model system was developed, by which microbial formation of volatiles could be examined The model was evaluated against dry, fermented sausages with respect to microbial growth, pH and volatile profiles. Fast and slowly acidified sausages and models were produced using the starte......H, microbial growth and volatile profiles was similar to sausage production. Based on these findings, the model system was considered valid for studies of aroma formation of meat cultures for fermented sausage.......A fermented meat model system was developed, by which microbial formation of volatiles could be examined The model was evaluated against dry, fermented sausages with respect to microbial growth, pH and volatile profiles. Fast and slowly acidified sausages and models were produced using the starter...... cultures Pediococcus pentosaceus and Staphylococcus xylosus. Volatiles were collected and analysed by dynamic headspace sampling and GC MS. The analysis was primarily focused on volatiles arising from amino acid degradation and a total of 24 compounds, of which 19 were quantified, were used...

  20. Uranium concentrations in natural waters, South Park, Colorado. [Part of National Uranium Resource Evaluation program

    Energy Technology Data Exchange (ETDEWEB)

    Sharp, R.R. Jr.; Aamodt, P.L.

    1976-08-01

    During the summer of 1975, 464 water samples from 149 locations in South Park, Colorado, were taken for the Los Alamos Scientific Laboratory in order to test the field sampling and analytical methodologies proposed for the NURE Hydrogeochemical and Stream Sediment Reconnaissance for uranium in the Rocky Mountain states and Alaska. The study showed, in the South Park area, that the analytical results do not vary significantly between samples which were untreated, filtered and acidified, filtered only, or acidified only. Furthermore, the analytical methods of fluorometry and delayed-neutron counting, as developed at the LASL for the reconnaissance work, provide fast, adequately precise, and complementary procedures for analyzing a broad range of uranium in natural waters. The data generated using this methodology does appear to identify uraniferous areas, and when applied using sound geochemical, geological, and hydrological principles, should prove a valuable tool in reconnaissance surveying to delineate new districts or areas of interest for uranium exploration.