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Sample records for electricity regulation cre

  1. Commission for Energy regulation (CRE) - Activity report June 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    CRE is the French commission for energy regulation. CRE's remit is to assist in ensuring the proper operation of the electricity and natural gas markets for the benefit of the end-user. In particular, CRE ensures that the conditions of access to electricity and natural gas transmission and distribution systems do not hinder the development of competition. It monitors, for the electricity and natural gas sectors, all transactions made between suppliers, traders and producers, all transactions made on the organised markets and cross-border trading. It ensures that suppliers, traders and producers propose offers that are consistent with their financial and technical constraints. It monitors the implementation of and compliance with regulations giving consumers the right to choose their supplier in a competitive market, and allowing new suppliers to enter the market. This document is the 2007 activity report of CRE. Content: A - Towards a single European energy market: Birth of a single European energy market (Origins of Europe of Energy, Emergence of a European energy policy); Main European Community guiding lines (European governance as regards energy, Guiding principles for the internal energy market); European Community activities (European Commission reports, Electricity and gas Regional Initiatives); Organisation and coordination of European regulators (Joint organisation of European regulators, CRE's relations with European Community institutions); CRE's European activities (Regional integration of gas markets, Regional integration of electricity markets, Operation of the European interconnected electricity grid and security of supply, CRE's other European activities); B - CRE action at national level: Grids/networks and infrastructures (General information, Electricity grids, Regulation of gas networks and infrastructures); Markets (Changes in the regulatory and legislative contexts of electricity and natural gas markets, Electricity markets

  2. Commission for Energy regulation (CRE) - Activity report June 2004

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    CRE is the French commission for energy regulation. CRE's remit is to assist in ensuring the proper operation of the electricity and natural gas markets for the benefit of the end-user. In particular, CRE ensures that the conditions of access to electricity and natural gas transmission and distribution systems do not hinder the development of competition. It monitors, for the electricity and natural gas sectors, all transactions made between suppliers, traders and producers, all transactions made on the organised markets and cross-border trading. It ensures that suppliers, traders and producers propose offers that are consistent with their financial and technical constraints. It monitors the implementation of and compliance with regulations giving consumers the right to choose their supplier in a competitive market, and allowing new suppliers to enter the market. This document is the 2004 activity report of CRE. Content: A - Opening of the gas and electricity markets for professional customers on 1 July 2004; B - Regulation of the gas market: Gas markets and players (The European environment, The French gas market); Regulation of the gas market (Implementing regulation, Works planned for the coming year; C - Regulation of the electricity market: The electricity markets and players (The European electricity markets, The French electricity market, Monitoring the electricity market); Regulation of the French electricity market (Access to public grid, Cross-border exchanges, Un-bundled accounting principles); The public electricity service in the regulated market (Content of the public service, Public service charges, Electricity production public service financing, Electricity sales tariffs) D - The working of CRE: How CRE exercises its jurisdiction, Tools; E - Appendices: Glossary, Units and conversions, Council of European Energy Regulators, Index of tables and figures.

  3. Commission for Energy regulation (CRE) - Activity report june 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    CRE is the French commission for energy regulation. CRE's remit is to assist in ensuring the proper operation of the electricity and natural gas markets for the benefit of the end-user. In particular, CRE ensures that the conditions of access to electricity and natural gas transmission and distribution systems do not hinder the development of competition. It monitors, for the electricity and natural gas sectors, all transactions made between suppliers, traders and producers, all transactions made on the organised markets and cross-border trading. It ensures that suppliers, traders and producers propose offers that are consistent with their financial and technical constraints. It monitors the implementation of and compliance with regulations giving consumers the right to choose their supplier in a competitive market, and allowing new suppliers to enter the market. This document is the 2006 activity report of CRE. Content: A - Opening of the electricity and natural gas markets to household consumers on 1 July 2007: CRE at the service of eligible customers (Information for eligible customers, Improved knowledge of non-household customers); Monitoring of the non-discrimination, transparency and independence of system operators (Drafting and distribution of codes of good conduct for system operators, The necessary improvement of system operator independence); Preparing the practical methods of opening: GTE 2007 and GTG 2007 (The necessary simplification of relations between operators and customers, Achieving a greater level of consumer information and protection, The clearly defined stages of the 'customer pathway', Profiling and settlement mechanisms: turning experience feedback from 2004 to good account); Persisting uncertainties and hurdles (The need for a suitable regulatory and legislative platform, Hurdles to the opening of the household market); B - Regulation of the natural gas market: The gas market in the European context (Increasing weight of

  4. Commission for Energy regulation (CRE) - Activity report june 2008; Commission de regulation de l'energie (CRE) - Rapport d'activite juin 2008

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    CRE is the French commission for energy regulation. CRE's remit is to assist in ensuring the proper operation of the electricity and natural gas markets for the benefit of the end-user. In particular, CRE ensures that the conditions of access to electricity and natural gas transmission and distribution systems do not hinder the development of competition. It monitors, for the electricity and natural gas sectors, all transactions made between suppliers, traders and producers, all transactions made on the organised markets and cross-border trading. It ensures that suppliers, traders and producers propose offers that are consistent with their financial and technical constraints. It monitors the implementation of and compliance with regulations giving consumers the right to choose their supplier in a competitive market, and allowing new suppliers to enter the market. This document is the 2008 activity report of CRE. Content: A - How CRE works: CRE regulatory authority and organisation: Powers, Organisation; Budget resources; Personnel; B - The Standing Committee for Dispute Settlement and Sanctions (CoRDiS) activity: Admissibility, Authority; C - Building a single European energy market: Overview; Organisation and coordination of the main European regulators (Work carried out collectively by European regulators, Regulator organisation and development, CRE's relations with European Community institutions, Development of CEER activities outside the European Union); CRE's European activities (The contribution of European regulators to the Third Energy Package, Integration of gas markets, Integration of electricity markets, Operation of the European interconnected electricity grid and security of supply, Opening up markets to benefit consumers); European Community activities (The European Commission's proposals for the internal energy market: the Third Energy Package, The European Commission's proposals for fighting climate change: the Climate

  5. Commission for Energy regulation (CRE) - Activity report June 2005; Commission de regulation de l'energie (CRE) - Rapport d'activite juin 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    CRE is the French commission for energy regulation. CRE's remit is to assist in ensuring the proper operation of the electricity and natural gas markets for the benefit of the end-user. In particular, CRE ensures that the conditions of access to electricity and natural gas transmission and distribution systems do not hinder the development of competition. It monitors, for the electricity and natural gas sectors, all transactions made between suppliers, traders and producers, all transactions made on the organised markets and cross-border trading. It ensures that suppliers, traders and producers propose offers that are consistent with their financial and technical constraints. It monitors the implementation of and compliance with regulations giving consumers the right to choose their supplier in a competitive market, and allowing new suppliers to enter the market. This document is the 2005 activity report of CRE. Content: A - The opening of the markets in France and in Europe: The opening of the markets one year after 1 July 2004 (An especially important step, Electricity and gas: a common framework with structural differences, The coexistence of market prices and regulated tariffs); The European texts of 26 June 2003 (Texts to give new impetus, Texts to harmonize the role and powers of national regulators, Texts to guarantee the independence of system operators, Texts to ensure transparent and non-discriminatory access to networks, Texts providing for strengthening of interconnections); The outlook for 2007, a fully open market (1 July 2007: a date set by the directives, Priority given to informing and protecting consumers); B - Regulation of the natural gas market: The gas market in the European context (Europe's dependency on imports is increasing, Gas prices increased considerably across the whole of Europe in 2004, The European gas scene continues to be dominated by a small number of players, Gas infrastructures need to be developed in Europe, The new

  6. Regulation of glycogen metabolism by the CRE-1, RCO-1 and RCM-1 proteins in Neurospora crassa. The role of CRE-1 as the central transcriptional regulator.

    Science.gov (United States)

    Cupertino, Fernanda Barbosa; Virgilio, Stela; Freitas, Fernanda Zanolli; Candido, Thiago de Souza; Bertolini, Maria Célia

    2015-04-01

    The transcription factor CreA/Mig1/CRE-1 is a repressor protein that regulates the use of alternative carbon sources via a mechanism known as Carbon Catabolite Repression (CCR). In Saccharomyces cerevisiae, Mig1 recruits the complex Ssn6-Tup1, the Neurospora crassa RCM-1 and RCO-1 orthologous proteins, respectively, to bind to promoters of glucose-repressible genes. We have been studying the regulation of glycogen metabolism in N. crassa and the identification of the RCO-1 corepressor as a regulator led us to investigate the regulatory role of CRE-1 in this process. Glycogen content is misregulated in the rco-1(KO), rcm-1(RIP) and cre-1(KO) strains, and the glycogen synthase phosphorylation is decreased in all strains, showing that CRE-1, RCO-1 and RCM-1 proteins are involved in glycogen accumulation and in the regulation of GSN activity by phosphorylation. We also confirmed the regulatory role of CRE-1 in CCR and its nuclear localization under repressing condition in N. crassa. The expression of all glycogenic genes is misregulated in the cre-1(KO) strain, suggesting that CRE-1 also controls glycogen metabolism by regulating gene expression. The existence of a high number of the Aspergillus nidulans CreA motif (5'-SYGGRG-3') in the glycogenic gene promoters led us to analyze the binding of CRE-1 to some DNA motifs both in vitro by DNA gel shift and in vivo by ChIP-qPCR analysis. CRE-1 bound in vivo to all motifs analyzed demonstrating that it down-regulates glycogen metabolism by controlling gene expression and GSN phosphorylation. Copyright © 2015 Elsevier Inc. All rights reserved.

  7. Deciphering the cis-regulatory elements for XYR1 and CRE1 regulators in Trichoderma reesei.

    Directory of Open Access Journals (Sweden)

    Rafael Silva-Rocha

    Full Text Available In this work, we report the in silico identification of the cis-regulatory elements for XYR1 and CRE1 proteins in the filamentous fungus Trichoderma reesei, two regulators that play a central role in the expression of cellulase genes. Using four datasets of condition-dependent genes from RNA-seq and RT-qPCR experiments, we performed unsupervised motif discovery and found two short motifs resembling the proposed binding consensus for XYR1 and CRE1. Using these motifs, we analysed the presence and arrangement of putative cis-regulatory elements recognized by both regulators and found that shortly spaced sites were more associated with XYR1- and CRE1-dependent promoters than single, high-score sites. Furthermore, the approach used here allowed the identification of the previously reported XYR1-binding sites from cel7a and xyn1 promoters, and we also mapped the potential target sequence for this regulator at the cel6a promoter that has been suggested but not identified previously. Additionally, seven other promoters (for cel7b, cel61a, cel61b, cel3c, cel3d, xyn3 and swo genes presented a putative XYR1-binding site, and strong sites for CRE1 were found at the xyr1 and cel7b promoters. Using the cis-regulatory architectures nearly defined for XYR1 and CRE1, we performed genome-wide identification of potential targets for direct regulation by both proteins and important differences on their functional regulons were elucidated. Finally, we performed binding site mapping on the promoters of differentially expressed genes found in T. reesei mutant strains lacking xyr1 or cre1 and found that indirect regulation plays a key role on their signalling pathways. Taken together, the data provided here sheds new light on the mechanisms for signal integration mediated by XYR1 and CRE1 at cellulase promoters.

  8. Generation of an Rx-tTA: TetOp-Cre knock-in mouse line for doxycycline regulated Cre activity in the Rx expression domain.

    Directory of Open Access Journals (Sweden)

    Timothy F Plageman

    Full Text Available Genetic deletion of mouse genes has played a crucial role in our understanding of embryonic eye development. Transgenic, tissue specific Cre recombinase expression in various eye structures has facilitated these experiments. However, an early expressing, temporally-regulated, optic vesicle-specific Cre line has not been available. In this report, we detail the generation and analysis of a knock-in, inducible Cre line designed to drive recombination specifically within the Rx expression domain. Crossing this line with a reporter line demonstrates that recombination can be mediated within the early optic vesicle and throughout retinal development. Recombination can also be mediated in the Rx-expressing, ventral diencephalon and future posterior pituitary gland. Furthermore, it was demonstrated that dietary doxycycline could effectively modulate Cre activity. This line has the potential to facilitate conditional knock-out experimentation to study early retina and/or posterior pituitary development.

  9. Overlapping CRE and E-box promoter elements can independently regulate COX-2 gene transcription in macrophages.

    Science.gov (United States)

    Mestre, J R; Rivadeneira, D E; Mackrell, P J; Duff, M; Stapleton, P P; Mack-Strong, V; Maddali, S; Smyth, G P; Tanabe, T; Daly, J M

    2001-05-11

    Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX-2 transcription. Endotoxin induced binding of nuclear proteins to the CRE and E-box and each element independently induced higher COX-2 transcription levels than the overlapping CRE/E-box. Transcription factors associated with the CRE binding complex included c-Jun and CRE binding protein and with the E-box binding complex USF-1; their overexpression significantly induced COX-2 transcription. Therefore, both CRE and E-box promoter elements regulate COX-2 transcription in macrophages.

  10. Decision from the Commission of Electricity Regulation (CRE) dated from June 27, 2002 about a disagreement between Semmaris company and Electricite de France, as manager of the public power distribution grid, relative to the conditions of consideration of the multiplicity of delivery points in the contractual system of access to the grid; Decision de la Commission de Regulation de l'Electricite (CRE) en date du 27 juin 2002 sur un different, qui oppose la societe Semmaris a Electricite de France, en tant que gestionnaire du reseau public de distribution d'electricite, relatif aux conditions de prise en compte de la multiplicite des points de livraison dans le dispositif contractuel d'acces au reseau electrique

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-06-01

    This document presents the analysis made by the French commission of electricity regulation (CRE) about a disagreement between Semmaris company (Rungis, France) and Electricite de France (EdF) concerning the request by Semmaris for the subscription of a single contract of access to the grid for its 18 supply points instead of 9 contracts as proposed by EdF after acceptation of a partial regrouping of some supply points under the same contracts. After examination of the hearings, the CRE requested EdF to establish non-discriminatory objective criteria for the regrouping of different delivery points in a same contract before making a new examination of the disagreement between Semmaris and EdF. (J.S.)

  11. A CRE1- regulated cluster is responsible for light dependent production of dihydrotrichotetronin in Trichoderma reesei.

    Directory of Open Access Journals (Sweden)

    Alberto Alonso Monroy

    Full Text Available Changing light conditions, caused by the rotation of earth resulting in day and night or growth on the surface or within a substrate, result in considerably altered physiological processes in fungi. For the biotechnological workhorse Trichoderma reesei, regulation of glycoside hydrolase gene expression, especially cellulase expression was shown to be a target of light dependent gene regulation. Analysis of regulatory targets of the carbon catabolite repressor CRE1 under cellulase inducing conditions revealed a secondary metabolite cluster to be differentially regulated in light and darkness and by photoreceptors. We found that this cluster is involved in production of trichodimerol and that the two polyketide synthases of the cluster are essential for biosynthesis of dihydrotrichotetronine (syn. bislongiquinolide or bisorbibutenolide. Additionally, an indirect influence on production of the peptaibol antibiotic paracelsin was observed. The two polyketide synthetase genes as well as the monooxygenase gene of the cluster were found to be connected at the level of transcription in a positive feedback cycle in darkness, but negative feedback in light, indicating a cellular sensing and response mechanism for the products of these enzymes. The transcription factor TR_102497/YPR2 residing within the cluster regulates the cluster genes in a light dependent manner. Additionally, an interrelationship of this cluster with regulation of cellulase gene expression was detected. Hence the regulatory connection between primary and secondary metabolism appears more widespread than previously assumed, indicating a sophisticated distribution of resources either to degradation of substrate (feed or to antagonism of competitors (fight, which is influenced by light.

  12. Electrical installations and regulations

    CERN Document Server

    Whitfield, J F

    1966-01-01

    Electrical Installations and Regulations focuses on the regulations that apply to electrical installations and the reasons for them. Topics covered range from electrical science to alternating and direct current supplies, as well as equipment for providing protection against excess current. Cables, wiring systems, and final subcircuits are also considered, along with earthing, discharge lighting, and testing and inspection.Comprised of 12 chapters, this book begins with an overview of electrical installation work, traits of a good electrician, and the regulations governing installations. The r

  13. The CRE1 Cytokinin Pathway Is Differentially Recruited Depending on Medicago truncatula Root Environments and Negatively Regulates Resistance to a Pathogen

    Science.gov (United States)

    Laffont, Carole; Rey, Thomas; André, Olivier; Novero, Mara; Kazmierczak, Théophile; Debellé, Frédéric; Bonfante, Paola; Jacquet, Christophe; Frugier, Florian

    2015-01-01

    Cytokinins are phytohormones that regulate many developmental and environmental responses. The Medicago truncatula cytokinin receptor MtCRE1 (Cytokinin Response 1) is required for the nitrogen-fixing symbiosis with rhizobia. As several cytokinin signaling genes are modulated in roots depending on different biotic and abiotic conditions, we assessed potential involvement of this pathway in various root environmental responses. Phenotyping of cre1 mutant roots infected by the Gigaspora margarita arbuscular mycorrhizal (AM) symbiotic fungus, the Aphanomyces euteiches root oomycete, or subjected to an abiotic stress (salt), were carried out. Detailed histological analysis and quantification of cre1 mycorrhized roots did not reveal any detrimental phenotype, suggesting that MtCRE1 does not belong to the ancestral common symbiotic pathway shared by rhizobial and AM symbioses. cre1 mutants formed an increased number of emerged lateral roots compared to wild-type plants, a phenotype which was also observed under non-stressed conditions. In response to A. euteiches, cre1 mutants showed reduced disease symptoms and an increased plant survival rate, correlated to an enhanced formation of lateral roots, a feature previously linked to Aphanomyces resistance. Overall, we showed that the cytokinin CRE1 pathway is not only required for symbiotic nodule organogenesis but also affects both root development and resistance to abiotic and biotic environmental stresses. PMID:25562779

  14. Conditional transgenesis using Dimerizable Cre (DiCre.

    Directory of Open Access Journals (Sweden)

    Nicolas Jullien

    Full Text Available Cre recombinase is extensively used to engineer the genome of experimental animals. However, its usefulness is still limited by the lack of an efficient temporal control over its activity. We have recently developed a conceptually new approach to regulate Cre recombinase, that we have called Dimerizable Cre or DiCre. It is based on splitting Cre into two inactive moieties and fusing them to FKBP12 (FK506-binding protein and FRB (binding domain of the FKBP12-rapamycin associated protein, respectively. These latter can be efficiently hetero-dimerized by rapamycin, leading to the reinstatement of Cre activity. We have been able to show, using in vitro approaches, that this ligand-induced dimerization is an efficient way to regulate Cre activity, and presents a low background activity together with a high efficiency of recombination following dimerization. To test the in vivo performance of this system, we have, in the present work, knocked-in DiCre into the Rosa26 locus of mice. To evaluate the performance of the DiCre system, mice have been mated with indicator mice (Z/EG or R26R and Cre-induced recombination was examined following activation of DiCre by rapamycin during embryonic development or after birth of progenies. No recombination could be observed in the absence of treatment of the animals, indicating a lack of background activity of DiCre in the absence of rapamycin. Postnatal rapamycin treatment (one to five daily injection, 10 mg/kg i.p induced recombination in a number of different tissues of progenies such as liver, heart, kidney, muscle, etc. On the other hand, recombination was at a very low level following in utero treatment of DiCrexR26R mice. In conclusion, DiCre has indeed the potentiality to be used to establish conditional Cre-deleter mice. An added advantage of this system is that, contrary to other modulatable Cre systems, it offers the possibility of obtaining regulated recombination in a combinatorial manner, i.e. induce

  15. An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes

    Directory of Open Access Journals (Sweden)

    Chitose Kurihara

    2016-12-01

    Full Text Available The recombinant adenoviral gene expression system is a powerful tool for gene delivery. However, it is difficult to obtain high titers of infectious virus, principally due to the toxicity of the expressed gene which affects on virus replication in the host HEK293 cells. To avoid these problems, we generated a Cre-loxP-regulated fluorescent universal vector (termed pAxCALRL. This vector produces recombinant adenoviruses that express the red fluorescent protein (RFP instead of the inserted gene during proliferation, which limits toxicity and can be used to monitor viral replication. Expression of the gene of interest is induced by co-infection with an adenovirus that expresses Cre-recombinase (AxCANCre. Recombinant adenovirus produced by this system that express Hnf4α and Foxa2 were used to reprogram mouse embryo fibroblast (MEF into induced-hepatocyte-like cells (iHep following several rounds of infection, demonstrating the efficacy of this new system.

  16. CRE communication on the work of the GTE 2007 and GTG 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-09-15

    The work conducted in the first half of 2006 by the Electricity Working Group (GTE) and Gas Working Group (GTG) consultation bodies set up by the Commission de regulation de l'energie (CRE), has specified the practical conditions for market operation defined in CRE communication of 10 January 2006. The CRE decision of 26 May 2005, has led to the creation of a consumer committee within the GTE and GTG allowing the expectations of gas and electricity consumers to be given greater consideration. During the first half of 2006, this consumer committee defined consumers needs in terms of information and protection, and set recommendations, whose application is one of the priorities over the coming months. The consumer committee has become a plenary committee for gas and electricity consumers, with the same stature as the GTE and GTG plenary committees. Following a round table with the players on 14 September 2006, CRE presents the decisions made and guidelines adopted for the coming months in this communication. (author)

  17. Hydrodynamic Delivery of Cre Protein to Lineage-Mark or Time-Stamp Mouse Hepatocytes In situ

    Science.gov (United States)

    Sonsteng, Katherine M.; Prigge, Justin R.; Talago, Emily A.; June, Ronald K.; Schmidt, Edward E.

    2014-01-01

    Cre-responsive fluorescent marker alleles are powerful tools for cell lineage tracing in mice; however their utility is limited by regulation of Cre activity. When targeting hepatocytes, hydrodynamic delivery of a Cre-expression plasmid can convert Cre-responsive alleles without inducing the intracellular or systemic antiviral responses often associated with viral-derived Cre-expression vectors. In this method, rapid high-volume intravenous inoculation induces hepatocyte-targeted uptake of extracellular molecules. Here we tested whether hydrodynamic delivery of Cre protein or Cre fused to the HIV-TAT cell-penetrating peptide could convert Cre-responsive reporters in hepatocytes of mice. Hydrodynamic delivery of 2 nmol of either Cre or TAT-Cre protein converted the reporter allele in 5 to 20% of hepatocytes. Neither protein gave detectable Cre activity in endothelia, non-liver organs, or non-hepatocyte cells in liver. Using mice homozygous for a Cre-responsive marker that directs red- (Cre-naïve) or green- (Cre-converted) fluorescent proteins to the nucleus, we assessed sub-saturation Cre-activity. One month after hydrodynamic inoculation with Cre protein, 58% of hepatocyte nuclei that were green were also red, indicating that less than half of the hepatocytes that had obtained enough Cre to convert one marker allele to green were able to convert all alleles. For comparison, one month after hydrodynamic delivery of a Cre-expression plasmid with a weak promoter, only 26% of the green nuclei were also red. Our results show that hydrodynamic delivery of Cre protein allows rapid allelic conversion in hepatocytes, but Cre-activity is sub-saturating so many cells will not convert multiple Cre-responsive alleles. PMID:24626158

  18. Split-Cre complementation restores combination activity on transgene excision in hair roots of transgenic tobacco.

    Directory of Open Access Journals (Sweden)

    Mengling Wen

    Full Text Available The Cre/loxP system is increasingly exploited for genetic manipulation of DNA in vitro and in vivo. It was previously reported that inactive ''split-Cre'' fragments could restore Cre activity in transgenic mice when overlapping co-expression was controlled by two different promoters. In this study, we analyzed recombination activities of split-Cre proteins, and found that no recombinase activity was detected in the in vitro recombination reaction in which only the N-terminal domain (NCre of split-Cre protein was expressed, whereas recombination activity was obtained when the C-terminal (CCre or both NCre and CCre fragments were supplied. We have also determined the recombination efficiency of split-Cre proteins which were co-expressed in hair roots of transgenic tobacco. No Cre recombination event was observed in hair roots of transgenic tobacco when the NCre or CCre genes were expressed alone. In contrast, an efficient recombination event was found in transgenic hairy roots co-expressing both inactive split-Cre genes. Moreover, the restored recombination efficiency of split-Cre proteins fused with the nuclear localization sequence (NLS was higher than that of intact Cre in transgenic lines. Thus, DNA recombination mediated by split-Cre proteins provides an alternative method for spatial and temporal regulation of gene expression in transgenic plants.

  19. Consultation organised by CRE and CREG on the France-Belgium interconnection; Consultation organisee par la CRE et la CREG relative a la gestion de l'interconnexion France-Belgique

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    In June 2002, RTE and ELIA put forward a proposal to CRE and CREG for setting up a new joint mechanism for allocating capacity on the Franco-Belgian border. As it contained a number of improvements, the regulators authorised its use as from July 1, 2002. At the same time, they announced that users would be consulted during Autumn 2002 on the way RTE's and ELIA's commitments had been reflected in contractual terms, the transparency of the allocation process, the new mechanism's overall efficiency and the possibility of allocating capacity on an annual basis. CRE and CREG ask everybody concerned by electricity transfers between France and Belgium to give his or her opinion on the way in which the interconnections new operating conditions contribute at present, or could contribute in the future, to developing fair and efficient competition on European Union electricity markets. (author)

  20. Synthesis of the investment program proposals submitted by RTE to CRE's approval; Synthese des propositions de programme d'investissement soumises par RTE a l'approbation de la CRE

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    Conformably to the French law from February 10, 2000, the French commission of electricity regulation (CRE) must proceed to the examination of the proposals submitted by the electricity transportation network (RTE) for the approval of its 2002 investments program devoted to the power grid. The main lines of the proposals submitted by RTE are described in this document: 1 - strategy of grid development (methodology, interconnections), 2 - methods of evaluation and selection of regional projects (replacement and development projects), 3 - main projects retained (main transportation network and interconnections, regional networks, remote control and safety systems), 4 - program financing plan. (J.S.)

  1. Competitive Electricity Market Regulation in the United States: A Primer

    Energy Technology Data Exchange (ETDEWEB)

    Flores-Espino, Francisco [National Renewable Energy Lab. (NREL), Golden, CO (United States); Tian, Tian [National Renewable Energy Lab. (NREL), Golden, CO (United States); Chernyakhovskiy, Ilya [National Renewable Energy Lab. (NREL), Golden, CO (United States); Chernyakhovskiy, Ilya [National Renewable Energy Lab. (NREL), Golden, CO (United States); Miller, Mackay [National Grid, Warwick (United Kingdom)

    2016-12-01

    The electricity system in the United States is a complex mechanism where different technologies, jurisdictions and regulatory designs interact. Today, two major models for electricity commercialization operate in the United States. One is the regulated monopoly model, in which vertically integrated electricity providers are regulated by state commissions. The other is the competitive model, in which power producers can openly access transmission infrastructure and participate in wholesale electricity markets. This paper describes the origins, evolution, and current status of the regulations that enable competitive markets in the United States.

  2. Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons.

    Directory of Open Access Journals (Sweden)

    Mandy Ka Man Kam

    Full Text Available To establish a genetic tool for conditional deletion or expression of gene in neurons in a temporally controlled manner, we generated a transgenic mouse (NSE-MerCreMer, which expressed a tamoxifen inducible type of Cre recombinase specifically in neurons. The tamoxifen inducible Cre recombinase (MerCreMer is a fusion protein containing Cre recombinase with two modified estrogen receptor ligand binding domains at both ends, and is driven by the neural-specific rat neural specific enolase (NSE promoter. A total of two transgenic lines were established, and expression of MerCreMer in neurons of the central and enteric nervous systems was confirmed. Transcript of MerCreMer was detected in several non-neural tissues such as heart, liver, and kidney in these lines. In the background of the Cre reporter mouse strain Rosa26R, Cre recombinase activity was inducible in neurons of adult NSE-MerCreMer mice treated with tamoxifen by intragastric gavage, but not in those fed with corn oil only. We conclude that NSE-MerCreMer lines will be useful for studying gene functions in neurons for the conditions that Cre-mediated recombination resulting in embryonic lethality, which precludes investigation of gene functions in neurons through later stages of development and in adult.

  3. aP2-Cre-mediated inactivation of estrogen receptor alpha causes hydrometra.

    Science.gov (United States)

    Antonson, Per; Matic, Marko; Portwood, Neil; Kuiper, Raoul V; Bryzgalova, Galyna; Gao, Hui; Windahl, Sara H; Humire, Patricia; Ohlsson, Claes; Berggren, Per-Olof; Gustafsson, Jan-Åke; Dahlman-Wright, Karin

    2014-01-01

    In this study we describe the reproductive phenotypes of a novel mouse model in which Cre-mediated deletion of ERα is regulated by the aP2 (fatty acid binding protein 4) promoter. ERα-floxed mice were crossed with transgenic mice expressing Cre-recombinase under the control of the aP2 promoter to generate aP2-Cre/ERα(flox/flox) mice. As expected, ERα mRNA levels were reduced in adipose tissue, but in addition we also detected an 80% reduction of ERα levels in the hypothalamus of aP2-Cre/ERα(flox/flox) mice. Phenotypic analysis revealed that aP2-Cre/ERα(flox/flox) female mice were infertile. In line with this, aP2-Cre/ERα(flox/flox) female mice did not cycle and presented 3.8-fold elevated estrogen levels. That elevated estrogen levels were associated with increased estrogen signaling was evidenced by increased mRNA levels of the estrogen-regulated genes lactoferrin and aquaporin 5 in the uterus. Furthermore, aP2-Cre/ERα(flox/flox) female mice showed an accumulation of intra-uterine fluid, hydrometra, without overt indications for causative anatomical anomalies. However, the vagina and cervix displayed advanced keratosis with abnormal quantities of accumulating squamous epithelial cells suggesting functional obstruction by keratin plugs. Importantly, treatment of aP2-Cre/ERα(flox/flox) mice with the aromatase inhibitor Letrozole caused regression of the hydrometra phenotype linking increased estrogen levels to the observed phenotype. We propose that in aP2-Cre/ERα(flox/flox) mice, increased serum estrogen levels cause over-stimulation in the uterus and genital tracts resulting in hydrometra and vaginal obstruction.

  4. High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.

    Science.gov (United States)

    Marciniak, Bogumiła C; Pabijaniak, Monika; de Jong, Anne; Dűhring, Robert; Seidel, Gerald; Hillen, Wolfgang; Kuipers, Oscar P

    2012-08-17

    In Bacillus subtilis and its relatives carbon catabolite control, a mechanism enabling to reach maximal efficiency of carbon and energy sources metabolism, is achieved by the global regulator CcpA (carbon catabolite protein A). CcpA in a complex with HPr-Ser-P (seryl-phosphorylated form of histidine-containing protein, HPr) binds to operator sites called catabolite responsive elements, cre. Depending on the cre box position relative to the promoter, the CcpA/HPr-Ser-P complex can either act as a positive or a negative regulator. The cre boxes are highly degenerate semi-palindromes with a lowly conserved consensus sequence. So far, studies aimed at revealing how CcpA can bind such diverse sites were focused on the analysis of single cre boxes. In this study, a genome-wide analysis of cre sites was performed in order to identify differences in cre sequence and position, which determine their binding affinity. The transcriptomes of B. subtilis cultures with three different CcpA expression levels were compared. The higher the amount of CcpA in the cells, the more operons possessing cre sites were differentially regulated. The cre boxes that mediated regulation at low CcpA levels were designated as strong (high affinity) and those which responded only to high amounts of CcpA, as weak (low affinity). Differences in the sequence and position in relation to the transcription start site between strong and weak cre boxes were revealed. Certain residues at specific positions in the cre box as well as, to a certain extent, a more palindromic nature of cre sequences and the location of cre in close vicinity to the transcription start site contribute to the strength of CcpA-dependent regulation. The main factors contributing to cre regulatory efficiencies, enabling subtle differential control of various subregulons of the CcpA regulon, are identified.

  5. High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis

    Directory of Open Access Journals (Sweden)

    Marciniak Bogumiła C

    2012-08-01

    Full Text Available Abstract Background In Bacillus subtilis and its relatives carbon catabolite control, a mechanism enabling to reach maximal efficiency of carbon and energy sources metabolism, is achieved by the global regulator CcpA (carbon catabolite protein A. CcpA in a complex with HPr-Ser-P (seryl-phosphorylated form of histidine-containing protein, HPr binds to operator sites called catabolite responsive elements, cre. Depending on the cre box position relative to the promoter, the CcpA/HPr-Ser-P complex can either act as a positive or a negative regulator. The cre boxes are highly degenerate semi-palindromes with a lowly conserved consensus sequence. So far, studies aimed at revealing how CcpA can bind such diverse sites were focused on the analysis of single cre boxes. In this study, a genome-wide analysis of cre sites was performed in order to identify differences in cre sequence and position, which determine their binding affinity. Results The transcriptomes of B. subtilis cultures with three different CcpA expression levels were compared. The higher the amount of CcpA in the cells, the more operons possessing cre sites were differentially regulated. The cre boxes that mediated regulation at low CcpA levels were designated as strong (high affinity and those which responded only to high amounts of CcpA, as weak (low affinity. Differences in the sequence and position in relation to the transcription start site between strong and weak cre boxes were revealed. Conclusions Certain residues at specific positions in the cre box as well as, to a certain extent, a more palindromic nature of cre sequences and the location of cre in close vicinity to the transcription start site contribute to the strength of CcpA-dependent regulation. The main factors contributing to cre regulatory efficiencies, enabling subtle differential control of various subregulons of the CcpA regulon, are identified.

  6. Disruption of Trichoderma reesei cre2, encoding an ubiquitin C-terminal hydrolase, results in increased cellulase activity

    Directory of Open Access Journals (Sweden)

    Denton Jai A

    2011-11-01

    Full Text Available Abstract Background The filamentous fungus Trichoderma reesei (Hypocrea jecorina is an important source of cellulases for use in the textile and alternative fuel industries. To fully understand the regulation of cellulase production in T. reesei, the role of a gene known to be involved in carbon regulation in Aspergillus nidulans, but unstudied in T. reesei, was investigated. Results The T. reesei orthologue of the A. nidulans creB gene, designated cre2, was identified and shown to be functional through heterologous complementation of a creB mutation in A. nidulans. A T. reesei strain was constructed using gene disruption techniques that contained a disrupted cre2 gene. This strain, JKTR2-6, exhibited phenotypes similar to the A. nidulans creB mutant strain both in carbon catabolite repressing, and in carbon catabolite derepressing conditions. Importantly, the disruption also led to elevated cellulase levels. Conclusions These results demonstrate that cre2 is involved in cellulase expression. Since the disruption of cre2 increases the amount of cellulase activity, without severe morphological affects, targeting creB orthologues for disruption in other industrially useful filamentous fungi, such as Aspergillus oryzae, Trichoderma harzianum or Aspergillus niger may also lead to elevated hydrolytic enzyme activity in these species.

  7. Comparison of Static Characteristics between Unregulated and Regulated Electrical Drives

    OpenAIRE

    Jukić Antolović, Jelena; Jerković Štil, Vedrana; Hederić, Željko

    2013-01-01

    In this paper, the difference between an unregulated and a regulated electrical drive in terms of power losses is analyzed. First, open loop scalar control, closed loop scalar control and speed estimated scalar control are explained. Then, static characteristics obtained by experimental measurements are analyzed and compared for different control principles. Finally, based on experimental results, it is proposed when and which drive is to be used.

  8. The CreB deubiquitinating enzyme does not directly target the CreA repressor protein in Aspergillus nidulans.

    Science.gov (United States)

    Alam, Md Ashiqul; Kamlangdee, Niyom; Kelly, Joan M

    2017-08-01

    Ubiquitination/deubiquitination pathways are now recognized as key components of gene regulatory mechanisms in eukaryotes. The major transcriptional repressor for carbon catabolite repression in Aspergillus nidulans is CreA, and mutational analysis led to the suggestion that a regulatory ubiquitination/deubiquitination pathway is involved. A key unanswered question is if and how this pathway, comprising CreB (deubiquitinating enzyme) and HulA (ubiquitin ligase) and other proteins, is involved in the regulatory mechanism. Previously, missense alleles of creA and creB were analysed for genetic interactions, and here we extended this to complete loss-of-function alleles of creA and creB, and compared morphological and biochemical phenotypes, which confirmed genetic interaction between the genes. We investigated whether CreA, or a protein in a complex with it, is a direct target of the CreB deubiquitination enzyme, using co-purifications of CreA and CreB, first using strains that overexpress the proteins and then using strains that express the proteins from their native promoters. The Phos-tag system was used to show that CreA is a phosphorylated protein, but no ubiquitination was detected using anti-ubiquitin antibodies and Western analysis. These findings were confirmed using mass spectrometry, which confirmed that CreA was differentially phosphorylated but not ubiquitinated. Thus, CreA is not a direct target of CreB, and nor are proteins that form part of a stable complex with CreA a target of CreB. These results open up new questions regarding the molecular mechanism of CreA repressing activity, and how the ubiquitination pathway involving CreB interacts with this regulatory network.

  9. Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors

    Directory of Open Access Journals (Sweden)

    Sablitzky Fred

    2004-01-01

    Full Text Available Abstract Background Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV and lentiviral (LV vectors into discrete regions of the forebrain. Results Recombinant AAV-Cre, AAV-GFP (green fluorescent protein and LV-Cre-EGFP (enhanced GFP were made with the transgene controlled by the cytomegalovirus promoter. Infecting 293T cells in vitro with AAV-Cre and LV-Cre-EGFP resulted in transduction of most cells as shown by GFP fluorescence and Cre immunoreactivity. Injections of submicrolitre quantities of LV-Cre-EGFP and mixtures of AAV-Cre with AAV-GFP into the neocortex and hippocampus of adult Rosa26 reporter mice resulted in strong Cre and GFP expression in the dentate gyrus and moderate to strong labelling in specific regions of the hippocampus and in the neocortex, mainly in neurons. The pattern of expression of Cre and GFP obtained with AAV and LV vectors was very similar. X-gal staining showed that Cre-mediated recombination had occurred in neurons in the same regions of the brain, starting at 3 days post-injection. No obvious toxic effects of Cre expression were detected even after four weeks post-injection. Conclusion AAV and LV vectors are capable of delivering Cre to neurons in discrete regions of the adult mouse brain and producing recombination.

  10. Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors.

    Science.gov (United States)

    Ahmed, Bushra Y; Chakravarthy, Sridhara; Eggers, Ruben; Hermens, Wim T J M C; Zhang, Jing Ying; Niclou, Simone P; Levelt, Christiaan; Sablitzky, Fred; Anderson, Patrick N; Lieberman, A R; Verhaagen, Joost

    2004-01-30

    Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV) and lentiviral (LV) vectors into discrete regions of the forebrain. Recombinant AAV-Cre, AAV-GFP (green fluorescent protein) and LV-Cre-EGFP (enhanced GFP) were made with the transgene controlled by the cytomegalovirus promoter. Infecting 293T cells in vitro with AAV-Cre and LV-Cre-EGFP resulted in transduction of most cells as shown by GFP fluorescence and Cre immunoreactivity. Injections of submicrolitre quantities of LV-Cre-EGFP and mixtures of AAV-Cre with AAV-GFP into the neocortex and hippocampus of adult Rosa26 reporter mice resulted in strong Cre and GFP expression in the dentate gyrus and moderate to strong labelling in specific regions of the hippocampus and in the neocortex, mainly in neurons. The pattern of expression of Cre and GFP obtained with AAV and LV vectors was very similar. X-gal staining showed that Cre-mediated recombination had occurred in neurons in the same regions of the brain, starting at 3 days post-injection. No obvious toxic effects of Cre expression were detected even after four weeks post-injection. AAV and LV vectors are capable of delivering Cre to neurons in discrete regions of the adult mouse brain and producing recombination.

  11. Economic regulation of electricity grids in Nordic countries

    Energy Technology Data Exchange (ETDEWEB)

    Robles, H.B.; EK, G. (Energy Markets Inspectorate, Eskilstuna (Sweden)); Ilonen, M.; Nurmi, S. (Energy Market Authority, Helsinki (Finland)); Moelgaard Jakobsen, N. (Danish Energy Regulatory Authority, Copenhagen (Denmark)); Syvertsen, S.C.; Steinnes, S.H. (Norwegian Water Resources and Energy Directorate, Oslo (Norway))

    2011-12-15

    This report is about the design of economic regulation of electricity companies in the Nordic countries. The purpose is to inform the interested reader on how the regulation of tariffs is designed. The intention is to give a short overview on the current economic regulation with an ambition to focus on differences and similarities. A common feature of the electricity distribution sector is that the industry structure consists of many independent companies with great differences in size and density of customers. This is contrary to what is common in other countries. The regulatory task can be more challenging with many separate utilities to regulate, especially if the industry is very heterogenous. In the appendices, the economic regulation of each country is presented in more detail. In the main text the focus is on differences and similarities. When comparing the regulations one can make two observations. On a superior level there are great similarities. All Nordic countries regulate the network companies by setting revenue caps. The legislation, the goals given to the regulators and the regulators general interpretation of the rules are to a great extent the same. The primary purposes are to prevent the monopolist to overcharge customers and to create a rational network industry. The regulation shall stimulate an effective management resulting in productivity development and optimal quality of the services. The differences in the Nordic economic regulations are in the details - in the setup of the regulatory models and choice of parameters. For instance; the assessment of a reasonable rate-of-return is done in all the regulations. When deciding this rate-of-return some countries use the method of weighted cost of capital (WACC) and capital asset pricing model (CAPM), other do not. Even when using the same method, the inputs in the model are not the same. Common for the regulatory models are the division of costs related to capital costs and operating costs. The

  12. Phosphorylation of connexin43 on serine 306 regulates electrical coupling

    DEFF Research Database (Denmark)

    Procida, Kristina; Jørgensen, Lone; Schmitt, Nicole

    2009-01-01

    BACKGROUND: Phosphorylation is a key regulatory event in controlling the function of the cardiac gap junction protein connexin43 (Cx43). Three new phosphorylation sites (S296, S297, S306) have been identified on Cx43; two of these sites (S297 and S306) are dephosphorylated during ischemia....... The functional significance of these new sites is currently unknown. OBJECTIVE: The purpose of this study was to examine the role of S296, S297, and S306 in the regulation of electrical intercellular communication. METHODS: To mimic constitutive dephosphorylation, serine was mutated to alanine at the three sites...... and expressed in HeLa cells. Electrical coupling and single channel measurements were performed by double patch clamp. Protein expression levels were assayed by western blotting, localization of Cx43, and phosphorylation of S306 by immunolabeling. Free hemichannels were assessed by biotinylation. RESULTS...

  13. A new Cre driver mouse line, Tcf21/Pod1-Cre, targets metanephric mesenchyme.

    Directory of Open Access Journals (Sweden)

    Yoshiro Maezawa

    Full Text Available Conditional gene targeting in mice has provided great insight into the role of gene function in kidney development and disease. Although a number of Cre-driver mouse strains already exist for the kidney, development of additional strains with unique expression patterns is needed. Here we report the generation and validation of a Tcf21/Pod1-Cre driver strain that expresses Cre recombinase throughout the condensing and stromal mesenchyme of developing kidneys and in their derivatives including epithelial components of the nephron and interstitial cells. To test the efficiency of this line, we crossed it to mice transgenic for either loss or gain of function β-catenin conditional alleles. Mice with deletion of β-catenin from Tcf21-expressing cells are born with hypoplastic kidneys, hydroureters and hydronephrosis. By contrast, Tcf21-Cre driven gain of function for β-catenin in mice results in fused midline kidneys and hypoplastic kidneys. Finally, we report the first renal mesenchymal deletion of Patched1 (Ptch1, the receptor for sonic hedgehog (Shh, which results in renal cysts demonstrating a functional role of Shh signaling pathway in renal cystogensis. In summary, we report the generation and validation of a new Cre driver strain that provides robust excision in metanephric mesenchyme.

  14. On the bill on modernization and development of electricity public service; Le projet de loi relatif a la modernisation et au developpement du service public de l'electricite (no. 1253)

    Energy Technology Data Exchange (ETDEWEB)

    Dumont, Jean-Louis [ed.] [Commission des Finances, de l' Economie generale et du Plan, Assemblee Nationale, Paris (France)

    1999-02-10

    This document is a report presented on behalf of Commission of Finances, General Economy and Planning of the French National Assembly about the bill on modernization and development of electricity public service. The report contains four parts. The first part is titled 'Reinforcing the independence of the management of transport public network'. The questions of independence of EDF and the autonomy of the grid management are addressed. Also, the issue of the transparency of the transport public network management is presented. The second part discusses the means of ensuring an efficient regulation of electricity market. The missions of the Electricity Regulation Commission (CRE) are presented in connection with tariff establishment, multi-annual investment planning and consulting role of CRE. CRE's role in arbitrating the conflicts and the endorsement of the judge of public service as well as the harmonizing the competences of CRE and Competition Council are also showed. The efficiency of regulation as resulting from a consolidated independence of CRE is demonstrated. The third part concerns reinforcing the electricity public service. The principles of electricity public service are exposed as well as requirements for increasing transparency in financing the public service obligations. The forth part concerns the ways of encouraging the participation of local communities in the organization of electric sector. The issues relating to mixed organization of electricity distribution, its modernization and the role of local communities with respect to supply distribution and environment protection are exposed.

  15. CreZOO--the European virtual repository of Cre and other targeted conditional driver strains.

    Science.gov (United States)

    Chandras, Christina; Zouberakis, Michael; Salimova, Ekaterina; Smedley, Damian; Rosenthal, Nadia; Aidinis, Vassilis

    2012-01-01

    The CreZOO (http://www.crezoo.org/) is the European virtual repository of Cre and other targeted conditional driver strains. These mice serve as tools for researchers to selectively 'switch off' gene expression in mouse models to examine gene function and disease pathology. CreZOO aims to capture and disseminate extant and new information on these Cre driver strains, such as genetic background and availability information, and details pertaining promoter, allele, inducibility and expression patterns, which are also presented. All transgenic strains carry detailed information according to MGI's official nomenclature, whereas their availability [e.g. live mice, cryopreserved embryos, sperm and embryonic stem (ES) cells] is clearly indicated with links to European and International databases and repositories (EMMA, MGI/IMSR, MMRRC, etc) and laboratories where the particular mouse strain is available together with the respective IDs. Each promoter/gene includes IDs and direct links to MGI, Entrez Gene, Ensembl, OMIM and RGD databases depending on their species origin, whereas allele information is presented with MGI IDs and active hyperlinks to redirect the user to the respective page in a new tab. The tissue/cell (special) and developmental (temporal) specificity expression patterns are clearly presented, whereas handling and genotyping details (in the form of documents or hyperlinks) together with all relevant publications are clearly presented with PMID(s) and direct PubMed links. CreZOO's design offers a user-friendly query interface and provides instant access to the list of conditional driver strains, promoters and inducibility details. Database access is free of charge and there are no registration requirements for data querying. CreZOO is being developed in the context of the CREATE consortium (http://www.creline.org/), a core of major European and international mouse database holders and research groups involved in conditional mutagenesis. Database URL: http

  16. Deletion of creB in Aspergillus oryzae increases secreted hydrolytic enzyme activity.

    Science.gov (United States)

    Hunter, A J; Morris, T A; Jin, B; Saint, C P; Kelly, J M

    2013-09-01

    Aspergillus oryzae has been used in the food and beverage industry for centuries, and industrial strains have been produced by multiple rounds of selection. Targeted gene deletion technology is particularly useful for strain improvement in such strains, particularly when they do not have a well-characterized meiotic cycle. Phenotypes of an Aspergillus nidulans strain null for the CreB deubiquitinating enzyme include effects on growth and repression, including increased activity levels of various enzymes. We show that Aspergillus oryzae contains a functional homologue of the CreB deubiquitinating enzyme and that a null strain shows increased activity levels of industrially important secreted enzymes, including cellulases, xylanases, amylases, and proteases, as well as alleviated inhibition of spore germination on glucose medium. Reverse transcription-quantitative PCR (RT-qPCR) analysis showed that the increased levels of enzyme activity in both Aspergillus nidulans and Aspergillus oryzae are mirrored at the transcript level, indicating transcriptional regulation. We report that Aspergillus oryzae DAR3699, originally isolated from soy fermentation, has a similar phenotype to that of a creB deletion mutant of the RIB40 strain, and it contains a mutation in the creB gene. Collectively, the results for Aspergillus oryzae, Aspergillus nidulans, Trichoderma reesei, and Penicillium decumbens show that deletion of creB may be broadly useful in diverse fungi for increasing production of a variety of enzymes.

  17. A Study of Plazomicin Compared With Colistin in Patients With Infection Due to Carbapenem-Resistant Enterobacteriaceae (CRE)

    Science.gov (United States)

    2016-10-03

    Bloodstream Infections (BSI) Due to CRE; Hospital-Acquired Bacterial Pneumonia (HABP) Due to CRE; Ventilator-Associated Bacterial Pneumonia (VABP) Due to CRE; Complicated Urinary Tract Infection (cUTI) Due to CRE; Acute Pyelonephritis (AP) Due to CRE

  18. Commission of energy regulation. 2004 activity report; Commission de regulation de l'energie. Rapport d'activite 2004

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    The commission of energy regulation (CRE) is an independent administrative authority in charge of the control of the operation of gas and electricity markets. This document is the fifth activity report of CRE and covers the July 1, 2003 - June 30, 2004 period, which corresponds to the era of opening of energy markets as a consequence of the enforcement of the June 26, 2003 European directive. In the framework of the stakes made by energy markets liberalization, this document presents the situation of the gas and electricity markets during this period (European framework, regulation of both markets, public utility mission..) and describes CRE's means for the monitoring of these markets. (J.S.)

  19. Modified Cre-loxP Recombination in Aspergillus oryzae by Direct Introduction of Cre Recombinase for Marker Gene Rescue

    Science.gov (United States)

    Masaki, Kazuo; Gomi, Katsuya; Iefuji, Haruyuki

    2012-01-01

    Marker rescue is an important molecular technique that enables sequential gene deletions. The Cre-loxP recombination system has been used for marker gene rescue in various organisms, including aspergilli. However, this system requires many time-consuming steps, including construction of a Cre expression plasmid, introduction of the plasmid, and Cre expression in the transformant. To circumvent this laborious process, we investigated a method wherein Cre could be directly introduced into Aspergillus oryzae protoplasts on carrier DNA such as a fragment or plasmid. In this study, we define the carrier DNA (Cre carrier) as a carrier for the Cre enzyme. A mixture of commercial Cre and nucleic acids (e.g., pUG6 plasmid) was introduced into A. oryzae protoplasts using a modified protoplast-polyethylene glycol method, resulting in the deletion of a selectable marker gene flanked by loxP sites. By using this method, we readily constructed a marker gene-rescued strain lacking ligD to optimize homologous recombination. Furthermore, we succeeded in integrative recombination at a loxP site in A. oryzae. Thus, we developed a simple method to use the Cre-loxP recombination system in A. oryzae by direct introduction of Cre into protoplasts using DNA as a carrier for the enzyme. PMID:22504800

  20. 76 FR 14587 - Defense Federal Acquisition Regulation Supplement; Multiyear Contract Authority for Electricity...

    Science.gov (United States)

    2011-03-17

    ... Regulation Supplement; Multiyear Contract Authority for Electricity From Renewable Energy Sources (DFARS Case... Defense to enter into a contract for a period not to exceed 10 years for the purchase of electricity from... prepared by DoD, that-- (1) The proposed purchase of electricity under such contract is cost effective; and...

  1. 75 FR 34942 - Defense Federal Acquisition Regulation Supplement; Multiyear Contract Authority for Electricity...

    Science.gov (United States)

    2010-06-21

    ... Regulation Supplement; Multiyear Contract Authority for Electricity From Renewable Energy Sources (DFARS Case... enter into a contract for a period not to exceed 10 years for the purchase of electricity from sources... electricity from sources of renewable energy, as that term is defined in section 203(b)(2) of the Energy...

  2. Regulation, Governance and Adaptation: Governance transformations in the Dutch and French liberalizing electricity industries

    NARCIS (Netherlands)

    E.M.M.I. Niesten (Eva)

    2009-01-01

    textabstractFor more than a decade, the European governments have focused their energy policies on creating one European competitive electricity market. Several regulations are introduced into the European electricity industries for this purpose: the energy firms have to unbundle the electricity

  3. Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes.

    Directory of Open Access Journals (Sweden)

    Jiong Zhang

    Full Text Available Herein we demonstrate with PCR, immunodetection and reporter gene approaches that the widely used human Glial Fibrillary Acidic Protein (hGFAP-Cre transgene exhibits spontaneous germ-line recombination activity in leading to deletion in brain, heart and tail tissue with high frequency. The ectopic activity of hGFAP-Cre requires a rigorous control. We likewise observed that a second widely used nestin-Cre transgene shows germ-line deletion. Here we describe procedures to identify mice with germ-line recombination mediated by the hGFAP-Cre and nestin-Cre transgenes. Such control is essential to avoid pleiotropic effects due to germ-line deletion of loxP-flanked target genes and to maintain the CNS-restricted deletion status in transgenic mouse colonies.

  4. Mutants of Cre recombinase with improved accuracy

    Science.gov (United States)

    Eroshenko, Nikolai; Church, George M.

    2013-01-01

    Despite rapid advances in genome engineering technologies, inserting genes into precise locations in the human genome remains an outstanding problem. It has been suggested that site-specific recombinases can be adapted towards use as transgene delivery vectors. The specificity of recombinases can be altered either with directed evolution or via fusions to modular DNA-binding domains. Unfortunately, both wildtype and altered variants often have detectable activities at off-target sites. Here we use bacterial selections to identify mutations in the dimerization surface of Cre recombinase (R32V, R32M, and 303GVSdup) that improve the accuracy of recombination. The mutants are functional in bacteria, in human cells, and in vitro (except for 303GVSdup, which we did not purify), and have improved selectivity against both model off-target sites and the entire E. coli genome. We propose that destabilizing binding cooperativity may be a general strategy for improving the accuracy of dimeric DNA-binding proteins. PMID:24056590

  5. DC microgrids providing frequency regulation in electrical power system - imperfect communication issues

    DEFF Research Database (Denmark)

    Bašić, Hrvoje; Dragicevic, Tomislav; Pandžić, Hrvoje

    2017-01-01

    This paper presents a model of multiple DC microgrids with battery energy storage systems and demand response capability, taking part in primary frequency regulation of electrical power system. Although DC microgrids can contribute to stability and efficiency of frequency regulation, these complex...... systems may cause serious stability issues due to the imperfect communication. This work presents possible scenarios of unstable primary frequency regulation in a simplified model of electrical power system with DC microgrids, which are controlled through communication network....

  6. Flavonoids and Auxin Transport Inhibitors Rescue Symbiotic Nodulation in the Medicago truncatula Cytokinin Perception Mutant cre1

    Science.gov (United States)

    Ng, Jason Liang Pin; Hassan, Samira; Truong, Thy T.; Hocart, Charles H.; Laffont, Carole; Frugier, Florian; Mathesius, Ulrike

    2015-01-01

    Initiation of symbiotic nodules in legumes requires cytokinin signaling, but its mechanism of action is largely unknown. Here, we tested whether the failure to initiate nodules in the Medicago truncatula cytokinin perception mutant cre1 (cytokinin response1) is due to its altered ability to regulate auxin transport, auxin accumulation, and induction of flavonoids. We found that in the cre1 mutant, symbiotic rhizobia cannot locally alter acro- and basipetal auxin transport during nodule initiation and that these mutants show reduced auxin (indole-3-acetic acid) accumulation and auxin responses compared with the wild type. Quantification of flavonoids, which can act as endogenous auxin transport inhibitors, showed a deficiency in the induction of free naringenin, isoliquiritigenin, quercetin, and hesperetin in cre1 roots compared with wild-type roots 24 h after inoculation with rhizobia. Coinoculation of roots with rhizobia and the flavonoids naringenin, isoliquiritigenin, and kaempferol, or with the synthetic auxin transport inhibitor 2,3,5,-triiodobenzoic acid, rescued nodulation efficiency in cre1 mutants and allowed auxin transport control in response to rhizobia. Our results suggest that CRE1-dependent cytokinin signaling leads to nodule initiation through the regulation of flavonoid accumulation required for local alteration of polar auxin transport and subsequent auxin accumulation in cortical cells during the early stages of nodulation. PMID:26253705

  7. SELECTION OF METHOD FOR REGULATION OF TRACTOR PROPULSION ASYNCHRONOUS ELECTRIC MOTOR AND CONSTRUCTION OF MECHANICAL CHARACTERISTICS

    Directory of Open Access Journals (Sweden)

    Ch. I. Zhdanovich

    2015-01-01

    Full Text Available Nowadays the work is in progress to develop wheeled and caterpillar tractors with electromechanical transmission. Range of changes in transmission gear ratio while using propulsion electric motor depends on mechanical characteristics of a tractor propulsion electric motor which is equipped with electromechanical transmission. In case when the range is rather high then it is possible to minimize number of gearings in the tractor gearing box or exclude its usage at all. Type of the applied propulsion electric motor and regulation method specify type of mechanical characteristics (characteristics family of the propulsion electric motor.The paper considers a propulsion asynchronous electric motor with frequency control. While using frequency control it is possible to regulate electric motor revolutions by mutual changes in voltage and voltage frequency. There are various laws of mutual changes in voltage and frequency (regulation laws. Selection of a regulation law influences on type of mechanical characteristics of a propulsion electric motor. Application of any law can be admissible only for some specific range of voltage frequency otherwise it is possible to exceed some parameters (for example, admissible voltage in the winding of electric motor stator. It is necessary to ensure the required moment within wide range for a tractor propulsion electric motor. In this case losses in the electric motor must be minimal. Losses in the rotor of the propulsion asynchronous electric motor are directly proportional to its sliding and its best propulsion and mechanical properties of a mobile machine will be ensured in the case when sliding is preserved at a constant value. According to these reasons selection of regulation laws has been carried out for operation of the propulsion asynchronous electric motor with nominal sliding and mechanical characteristics at nominal sliding is conventionally called a nominal characteristics.The paper analyzes the possible

  8. Targeting of GFP-Cre to the mouse Cyp11a1 locus both drives cre recombinase expression in steroidogenic cells and permits generation of Cyp11a1 knock out mice.

    Directory of Open Access Journals (Sweden)

    Laura O'Hara

    Full Text Available To permit conditional gene targeting of floxed alleles in steroidogenic cell-types we have generated a transgenic mouse line that expresses Cre Recombinase under the regulation of the endogenous Cytochrome P450 side chain cleavage enzyme (Cyp11a1 promoter. Mice Carrying the Cyp11a1-GC (GFP-Cre allele express Cre Recombinase in fetal adrenal and testis, and adrenal cortex, testicular Leydig cells (and a small proportion of Sertoli cells, theca cells of the ovary, and the hindbrain in postnatal life. Circulating testosterone concentration is unchanged in Cyp11(+/GC males, suggesting steroidogenesis is unaffected by loss of one allele of Cyp11a1, mice are grossly normal, and Cre Recombinase functions to recombine floxed alleles of both a YFP reporter gene and the Androgen Receptor (AR in steroidogenic cells of the testis, ovary, adrenal and hindbrain. Additionally, when bred to homozygosity (Cyp11a1(GC/GC , knock-in of GFP-Cre to the endogenous Cyp11a1 locus results in a novel mouse model lacking endogenous Cyp11a1 (P450-SCC function. This unique dual-purpose model has utility both for those wishing to conditionally target genes within steroidogenic cell types and for studies requiring mice lacking endogenous steroid hormone production.

  9. the rule of law approach to regulating electricity supply in nigeria

    African Journals Online (AJOL)

    RAYAN_

    THE RULE OF LAW APPROACH TO REGULATING. ELECTRICITY SUPPLY IN NIGERIA. Sam Amadi*. ABSTRACT. With the transfer of government shares in 17 electricity generation and distribution companies in November 2013, Nigeria crossed the Rubicon in the privatization process. This process began in earnest in ...

  10. Regulation and conflicts regarding electricity interconnectors; Regulering en conflicten rondom landsgrensoverschrijdende elektriciteitsnetten

    Energy Technology Data Exchange (ETDEWEB)

    Aarts, V.P. [Brauw Blackstone Westbroek, Den Haag (Netherlands); Wenting, F. [Transmission System Oprator TenneT, Arnhem (Netherlands)

    2003-02-01

    In this contribution the authors provide an overview of regulatory issues and conflicts regarding electricity interconnectors between the Netherlands and its neighbours. The Electricity Act 1998 has assigned management of the Dutch part of the interconnectors to exclusively TenneT, the national transmission system operator in the Netherlands. The authors conclude that the dust of many regulatory problems and conflicts concerning existing interconnectors has settled down. The preliminary proceedings regarding the long Sep priority which are pending with the EU Court of Justice must still produce an answer as to the compatibility of this priority with community law. Regarding construction and management of new interconnectors many regulatory and commercial challenges lie ahead. As regards congestion management on existing interconnectors the authors raise the question whether concentration on increasing physical interconnector capacity is the right approach. Interconnector capacity, even if substantially expanded, will continue to be liable to falling short of demand due to price differences and volatility of prices in the various national markets. Perhaps more effort should be put into attaining conditions for non-transaction based congestion management. The combined UCTE (Union for the Co-ordination of Transmission of Electricity) grid is, technically speaking, a 'copper plate'. If it were only managed as such by the various national transmission system operators through co-ordinated control of production and load, almost all transports on this copper plate could be secured, irrespective of underlying commercial flows. [Dutch] De landsgrensoverschrijdende electriciteitsnetten ziin van cruciaal belang voor liberalisering op Europese schaal: ze vormen als het ware de snelwegen waarover buiten Nederland opgewekte of ingekochte stroorn zijn weg naar de Nederlandse afzetmarkt kan vinden en waarover, omgekeerd, de stroorn die in Nederland wordt geproduceerd of

  11. Regulation of Electric Power Generation while Improving TTP Use Efficiency

    Directory of Open Access Journals (Sweden)

    V. N. Romaniuk

    2012-01-01

    Full Text Available Taking integrated power system of theRepublicofBelarusas an example the paper considers a possibility of heat accumulator use for TPP operation in accordance with the schedule of electric power consumption while maintaining daily supply of heat energy from turbine power takes-off and without involvement of peak-loader boilers used for covering energy loads.

  12. Potential Analysis of Regulating Power from Electric Vehicle (EV) Integration in Denmark

    DEFF Research Database (Denmark)

    Wu, Qiuwei; Nielsen, Arne Hejde; Østergaard, Jacob

    2011-01-01

    The potential analysis of having electric vehicles (EVs) provide regulating power has been implemented. The possible regulating power capacity from EVs and the economic return for EV users by providing regulating power are obtained. A spot price based charging schedule scenario has been used to do...... the day-ahead charging schedule for all EVs based on the predicted driving pattern. On top of the obtained charging schedule, the predicted EV availability and the charging schedule are used to calculate the possible regulating power capacity from EVs for both up regulating power and down regulating power...

  13. Negative Effects of Antimonopoly Regulation on the Russian Electric Power Industry

    Directory of Open Access Journals (Sweden)

    Elena NEPRINTSEVA

    2017-07-01

    Full Text Available With the antimonopoly regulation in the domestic economy getting more stringent an analysis of the current measures of antimonopoly regulation in terms of their efficiency is now becoming ever more relevant. The aim of the study - analyze how the measures of antimonopoly regulation affect competitive relationships in the electric power industry. The following methods have been used in this work: empirical method, cause-effect method and scientific abstraction method. The article sets out an analysis of the antimonopoly regulation measures that the antimonopoly authority applies. It also provides an assessment of consequences that follow from such methods being applied for the promotion of competitive relationships on the market of electric power and capacity. A conclusion has been reached that the antimonopoly regulation measures being applied impede the progress of competitive relationships on the market of electric power and capacity. The continuing process of reformation in electric power industry aims to liberalize relationships in the area of electric power production. Yet, as a result of this process, generating capacities are becoming increasingly more concentrated mainly around state companies. This is mainly caused by the lack of certainty regarding the results of the industry reformation and a more stringent state regulation over the last years of the reforms. At the same time, for the purposes of limiting the market force, measures of antimonopoly regulation are being applied to generating companies. Such measures have an adverse effect on competitive relationships and stimulate further concentration.

  14. Strict control of telomerase activation using Cre-mediated inversion

    Directory of Open Access Journals (Sweden)

    Harrington Lea

    2006-02-01

    Full Text Available Abstract Background Human cells appear exquisitely sensitive to the levels of hTERT expression, the telomerase reverse transcriptase. In primary cells that do not express hTERT, telomeres erode with each successive cell division, leading to the eventual loss of telomere DNA, an induction of a telomere DNA damage response, and the onset of cellular senescence or crisis. In some instances, an average of less than one appropriately spliced hTERT transcript per cell appears sufficient to restore telomerase activity and telomere maintenance, and overcome finite replicative capacity. Results To underscore this sensitivity, we showed that a widely used system of transcriptional induction involving ecdysone (muristerone led to sufficient expression of hTERT to immortalize human fibroblasts, even in the absence of induction. To permit tightly regulated expression of hTERT, or any other gene of interest, we developed a method of transcriptional control using an invertible expression cassette flanked by antiparallel loxP recombination sites. When introduced into human fibroblasts with the hTERT cDNA positioned in the opposite orientation relative to a constitutively active promoter, no telomerase activity was detected, and the cell population retained a mortal phenotype. Upon inversion of the hTERT cDNA to a transcriptionally competent orientation via the action of Cre recombinase, cells acquired telomerase activity, telomere DNA was replenished, and the population was immortalized. Further, using expression of a fluorescent protein marker, we demonstrated the ability to repeatedly invert specific transcripts between an active and inactive state in an otherwise isogenic cell background. Conclusion This binary expression system thus provides a useful genetic means to strictly regulate the expression of a given gene, or to control the expression of at least two different genes in a mutually exclusive manner.

  15. Heat-inducible Cre-lox system for marker excision in transgenic rice

    Indian Academy of Sciences (India)

    The present study assessed the efficacy of a heat-inducible cre gene for conditional removal of the marker gene from a rice genome via Cre-lox recombination. A cre gene controlled by the soybean heat-shock promoter was introduced into the rice genome along with the recombination target (lox) construct. Cre-mediated ...

  16. Fast regulation of axonal growth cone motility by electrical activity.

    Science.gov (United States)

    Ibarretxe, Gaskon; Perrais, David; Jaskolski, Frédéric; Vimeney, Alice; Mulle, Christophe

    2007-07-18

    Axonal growth cones are responsible for the correct guidance of developing axons and the establishment of functional neural networks. They are highly motile because of fast and continuous rearrangements of their actin-rich cytoskeleton. Here we have used live imaging of axonal growth cones of hippocampal neurons in culture and quantified their motility with a temporal resolution of 2 s. Using novel methods of analysis of growth cone dynamics, we show that transient activation of kainate receptors by bath-applied kainate induced a fast and reversible growth cone stalling. This effect depends on electrical activity and can be mimicked by the transient discharge of action potentials elicited in the neuron by intracellular current injections at the somatic level through a patch pipette. Growth cone stalling induced by electrical stimulation is mediated by calcium entry from the extracellular medium as well as by calcium release from intracellular stores that define spatially restricted microdomains directly affecting cytoskeletal dynamics. We propose that growth cone motility is dynamically controlled by transient bursts of spontaneous electrical activity, which constitutes a prominent feature of developing neural networks in vivo.

  17. Lipid nanotube formation using space-regulated electric field above interdigitated electrodes.

    Science.gov (United States)

    Bi, Hongmei; Fu, Dingguo; Wang, Lei; Han, Xiaojun

    2014-04-22

    Lipid nanotubes have great potential in biology and nanotechnology. Here we demonstrate a method to form lipid nanotubes using space-regulated AC electric fields above coplanar interdigitated electrodes. The AC electric field distribution can be regulated by solution height above the electrodes. The ratio of field component in x axis (Ex) to field component in z axis (Ez) increases dramatically at solution height below 50 μm; therefore, at lower solution height, the force from Ex predominantly drives lipids to form lipid nanotubes along with the electric field direction. The forces exerted on the lipid nanotube during its formation were analyzed in detail, and an equation was obtained to describe the relationship among nanotube length and field frequency, amplitude, and time. We believe that the presented approach opens a way to design and prepare nanoscale materials with unique structural and functional properties using space-regulated electric fields.

  18. From franchise to state commission: Regulation of the electric utility industry, 1907 to 1932

    Science.gov (United States)

    Reutter, Keith Alan

    1997-09-01

    Empirical research into the effects of regulation on industry has been around since the early 1960s. Over the last thirty plus years a number of interesting results have been brought to the fore. For instance, it has been found that regulation of the trucking industry limits entry and increases prices. A similar result has been pointed to in other industries such as commercial airlines and banking. The effect of the state commission form of regulation on the electric utility industry has been less conclusive. State commissions became dominant during the period 1910-1930, replacing local franchising as a method of regulating the electric utility industry. Two competing theories suggest why this transformation took place, the "capture" and "public interest" theories of regulation. The capture theory of regulation suggests that the electric utility industry demanded state regulation as a way to earn above normal profits and reduce competition. The public interest theory suggests the purpose of regulation by state commissions was to benefit the general public by forcing the industry to be competitive. Few studies have tried to determine which theory more aptly describes the actual events that took place. The empirical model developed in Chapter V, is an extension of the current literature. A set of simultaneous equations describing the natural gas and electricity markets is estimated using cross-sectional time-series data from 1907 to 1932. The effect of regulation on the electric utility industry is modeled with a dummy variable taking on a value of one to designate that a state commission had been established. The results suggest the capture theory of regulation best describes the period under study. The empirical estimates indicate that state commissions (1) reduced the rate at which the real price of electricity was falling, (2) had a negative impact on firms entering the industry, (3) had a positive influence on the cost of producing a kwh of electricity, and (4

  19. Long-term Cre-mediated Retrograde Tagging of Neurons Using a Novel Recombinant Pseudorabies Virus

    Directory of Open Access Journals (Sweden)

    Hassana eOyibo

    2014-09-01

    Full Text Available Brain regions contain diverse populations of neurons that project to different long-range targets. The study of these subpopulations in circuit function and behavior requires a toolkit to characterize and manipulate their activity in vivo. We have developed a novel set of reagents based on Pseudorabies Virus (PRV for efficient and long-term genetic tagging of neurons based on their projection targets. By deleting IE180, the master transcriptional regulator in the PRV genome, we have produced a mutant virus capable of infection and transgene expression in neurons but unable to replicate in or spread from those neurons. IE180-null mutants showed no cytotoxicity, and infected neurons exhibited normal physiological function more than 45 days after infection, indicating the utility of these engineered viruses for chronic experiments. To enable rapid and convenient construction of novel IE180-null recombinants, we engineered a bacterial artificial chromosome (BAC shuttle-vector system for moving new constructs into the PRV IE180-null genome. Using this system we generated an IE180-null recombinant virus expressing the site-specific recombinase Cre. This Cre-expressing virus (PRV-hSyn-Cre efficiently and robustly infects neurons in vivo and activates transgene expression from Cre-dependent vectors in local and retrograde projecting populations of neurons in the mouse. We also generated an assortment of recombinant viruses expressing fluorescent proteins (mCherry, EGFP, ECFP. These viruses exhibit long-term labeling of neurons in vitro but transient labeling in vivo. Together these novel IE180-null PRV reagents expand the toolkit for targeted gene expression in the brain, facilitating functional dissection of neuronal circuits in vivo.

  20. Flexible Consumers Reserving Electricity and Offering Profitable Downward Regulation

    NARCIS (Netherlands)

    N.F. Höning (Nicolas); J.A. La Poutré (Han); K. Strunz

    2012-01-01

    htmlabstractPrevious work on demand response in smart grids considers dynamic real-time prices, but has so far neglected to consider how consumers can also be involved in planning ahead, both for scheduling of consumption and reserving their ability to regulate downward during balancing. This work

  1. A review of international abuse testing standards and regulations for lithium ion batteries in electric and hybrid electric vehicles

    OpenAIRE

    RUIZ RUIZ VANESA; PFRANG ANDREAS; KRISTON AKOS; OMAR NOSHIM; VAN DEN BOSSCHE PETER; BRETT LOIS

    2015-01-01

    Lithium ion batteries are a proven technology for automotive applications and their continued use in the future electric vehicle fleet is undeniable. In addition to battery performance and durability, battery safety is paramount to ensure confidence and widespread adoption of electromobility in our society. This comprehensive review aims at presenting the various international standards and regulations for safety testing of lithium ion batteries in automotive applications under various abusiv...

  2. Collaborative jurisdiction in the regulation of electric utilities: A new look at jurisdictional boundaries

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1991-12-31

    This conference is one of several activities initiated by FERC, DOE and NARUC to improve the dialogue between Federal and State regulators and policymakers. I am pleased to be here to participate in this conference and to address, with you, electricity issues of truly national significance. I would like to commend Ashley Brown and the NARUC Electricity Committee for its foresight in devising a conference on these issues at this critical juncture in the regulation of the electric utility industry. I also would like to commend Chairman Allday and the FERC for their efforts to improve communication between Federal and State electricity regulators; both through FERC`s Public Conference on Electricity Issues that was held last June, and through the FERC/NARUC workshops that are scheduled to follow this conference. These collaborative efforts are important and necessary steps in addressing successfully the many issues facing the electric utility industry those who regulate it, and those who depend upon it - in other words, about everyone.

  3. Imaging Voltage in Genetically Defined Neuronal Subpopulations with a Cre Recombinase-Targeted Hybrid Voltage Sensor.

    Science.gov (United States)

    Bayguinov, Peter O; Ma, Yihe; Gao, Yu; Zhao, Xinyu; Jackson, Meyer B

    2017-09-20

    Genetically encoded voltage indicators create an opportunity to monitor electrical activity in defined sets of neurons as they participate in the complex patterns of coordinated electrical activity that underlie nervous system function. Taking full advantage of genetically encoded voltage indicators requires a generalized strategy for targeting the probe to genetically defined populations of cells. To this end, we have generated a mouse line with an optimized hybrid voltage sensor (hVOS) probe within a locus designed for efficient Cre recombinase-dependent expression. Crossing this mouse with Cre drivers generated double transgenics expressing hVOS probe in GABAergic, parvalbumin, and calretinin interneurons, as well as hilar mossy cells, new adult-born neurons, and recently active neurons. In each case, imaging in brain slices from male or female animals revealed electrically evoked optical signals from multiple individual neurons in single trials. These imaging experiments revealed action potentials, dynamic aspects of dendritic integration, and trial-to-trial fluctuations in response latency. The rapid time response of hVOS imaging revealed action potentials with high temporal fidelity, and enabled accurate measurements of spike half-widths characteristic of each cell type. Simultaneous recording of rapid voltage changes in multiple neurons with a common genetic signature offers a powerful approach to the study of neural circuit function and the investigation of how neural networks encode, process, and store information. SIGNIFICANCE STATEMENT Genetically encoded voltage indicators hold great promise in the study of neural circuitry, but realizing their full potential depends on targeting the sensor to distinct cell types. Here we present a new mouse line that expresses a hybrid optical voltage sensor under the control of Cre recombinase. Crossing this line with Cre drivers generated double-transgenic mice, which express this sensor in targeted cell types. In

  4. Heterogeneous transgene expression in the retinas of the TH-RFP, TH-Cre, TH-BAC-Cre and DAT-Cre mouse lines.

    Science.gov (United States)

    Vuong, H E; Pérez de Sevilla Müller, L; Hardi, C N; McMahon, D G; Brecha, N C

    2015-10-29

    Transgenic mouse lines are essential tools for understanding the connectivity, physiology and function of neuronal circuits, including those in the retina. This report compares transgene expression in the retina of a tyrosine hydroxylase (TH)-red fluorescent protein (RFP) mouse line with three catecholamine-related Cre recombinase mouse lines [TH-bacterial artificial chromosome (BAC)-, TH-, and dopamine transporter (DAT)-Cre] that were crossed with a ROSA26-tdTomato reporter line. Retinas were evaluated and immunostained with commonly used antibodies including those directed to TH, GABA and glycine to characterize the RFP or tdTomato fluorescent-labeled amacrine cells, and an antibody directed to RNA-binding protein with multiple splicing to identify ganglion cells. In TH-RFP retinas, types 1 and 2 dopamine (DA) amacrine cells were identified by their characteristic cellular morphology and type 1 DA cells by their expression of TH immunoreactivity. In the TH-BAC-, TH-, and DAT-tdTomato retinas, less than 1%, ∼ 6%, and 0%, respectively, of the fluorescent cells were the expected type 1 DA amacrine cells. Instead, in the TH-BAC-tdTomato retinas, fluorescently labeled AII amacrine cells were predominant, with some medium diameter ganglion cells. In TH-tdTomato retinas, fluorescence was in multiple neurochemical amacrine cell types, including four types of polyaxonal amacrine cells. In DAT-tdTomato retinas, fluorescence was in GABA immunoreactive amacrine cells, including two types of bistratified and two types of monostratified amacrine cells. Although each of the Cre lines was generated with the intent to specifically label DA cells, our findings show a cellular diversity in Cre expression in the adult retina and indicate the importance of careful characterization of transgene labeling patterns. These mouse lines with their distinctive cellular labeling patterns will be useful tools for future studies of retinal function and visual processing. Published by Elsevier

  5. THE STUDY OF TRANSIENT PROCESSES OF THE FREQUENCY-REGULATED SYNCHRONOUS ELECTRIC DRIVE

    OpenAIRE

    B. I. Firago; S. V. Aleksandrovsky

    2016-01-01

    In order to improve the power indices and to simplify the system of frequency speed control the scope of application of synchronous variable-frequency electric drives with independent frequency setting is being expanded. The synchronous motors with electromagnetic excitation and permanent magnet excitation in various industrial settings, including load-lifting machines and mechanisms, are used. As compared with the asynchronous frequency-regulated electric drives the synchronous ones have low...

  6. Institutional aspects of state regulation of interrelations of subjects of the electric energy market

    Directory of Open Access Journals (Sweden)

    Kuzmynchuk Nataliia V.

    2014-01-01

    Full Text Available The article identifies tendencies of development of international electric energy markets. It marks out the role of well-organised functioning of the electric energy market for maintaining the energy security of the country under complex conditions of Ukrainian economy transformation under the influence of geopolitical changes, financial, economic and social factors. It justifies important role of the state in realisation of the regulating impact through mechanisms of institutional support of production, transportation and supply of electric energy; it focuses on imperfection of the existing system of institutes of this sphere, which determine harmonicity of the electric energy buy and sell process. Based on the system analysis of the institutional foundation, which reflects principles and essence of regulation of the electric energy market and interrelations of its subjects, the article generalises problems of the modern regulatory and legal base and realisation of strategic programmes of development in the context of formation of relations between the energy market participants, which would facilitate renovation of state regulation. The article marks out key directions of solution of topical issues of institutional support of activity of electric energy market participants, the most urgent of which are problems of the tariff policy, alternative energy development, financial discipline and technical state of fixed assets of the electric energy complex. The article puts in order the system of contractual relations of subjects of the electric energy market, which allows formation of the scientifically justified approaches in the direction of improvement of the organisational structure of the energy market and introduction of measures on increase of efficiency of regulating actions by the state.

  7. Tariff regulation models of the electric sector; Modelos de regulacao tarifaria do setor eletrico

    Energy Technology Data Exchange (ETDEWEB)

    Pires, Jose Claudio Linhares; Piccinini, Mauricio Serrao

    2003-07-01

    This article discusses the tariff regulation models adopted in the electricity utility sector, with a focus on the innovations introduced as a result of the liberalization of the sector that began in the 1980s. The principal tariff criteria are discussed: tariffs determined by the both the rate of return regulation and by marginal cost, and price-caps. Instruments complementary to tariffs are also examined. The main aim of the article is to contribute to a better understanding of the tariff rules adopted in the electricity sector. (author)

  8. Electrical signals as mechanism of photosynthesis regulation in plants.

    Science.gov (United States)

    Sukhov, Vladimir

    2016-12-01

    This review summarizes current works concerning the effects of electrical signals (ESs) on photosynthesis, the mechanisms of the effects, and its physiological role in plants. Local irritations of plants induce various photosynthetic responses in intact leaves, including fast and long-term inactivation of photosynthesis, and its activation. Irritation-induced ESs, including action potential, variation potential, and system potential, probably causes the photosynthetic responses in intact leaves. Probable mechanisms of induction of fast inactivation of photosynthesis are associated with Ca(2+)- and (or) H(+)-influxes during ESs generation; long-term inactivation of photosynthesis might be caused by Ca(2+)- and (or) H(+)-influxes, production of abscisic and jasmonic acids, and inactivation of phloem H(+)-sucrose symporters. It is probable that subsequent development of inactivation of photosynthesis is mainly associated with decreased CO2 influx and inactivation of the photosynthetic dark reactions, which induces decreased photochemical quantum yields of photosystems I and II and increased non-photochemical quenching of photosystem II fluorescence and cyclic electron flow around photosystem I. However, other pathways of the ESs influence on the photosynthetic light reactions are also possible. One of them might be associated with ES-connected acidification of chloroplast stroma inducing ferredoxin-NADP(+) reductase accumulation at the thylakoids in Tic62 and TROL complexes. Mechanisms of ES-induced activation of photosynthesis require further investigation. The probable ultimate effect of ES-induced photosynthetic responses in plant life is the increased photosynthetic machinery resistance to stressors, including high and low temperatures, and enhanced whole-plant resistance to environmental factors at least during 1 h after irritation.

  9. CRISPR/Cas9-mediated targeting of the Rosa26 locus produces Cre reporter rat strains for monitoring Cre-loxP-mediated lineage tracing.

    Science.gov (United States)

    Ma, Yuanwu; Yu, Lei; Pan, Shuo; Gao, Shan; Chen, Wei; Zhang, Xu; Dong, Wei; Li, Jing; Zhou, Rui; Huang, Lan; Han, Yunlin; Bai, Lin; Zhang, Li; Zhang, Lianfeng

    2017-10-01

    The rat is an important laboratory animal for physiological, toxicological and pharmacological studies. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) is a simple and efficient tool to generate precise genetic modifications in rats, which will promote the accumulation of genetic resources and enable more precise studies of gene function. To monitor Cre-loxP-mediated excision in vivo, we generated a Cre reporter rat strain (Rosa26-imCherry) by knockin of a Cre reporter cassette at the Rosa26 locus using CRISPR/Cas9. Rosa26-imCherry rats exhibited inducible expression of the mCherry cassette (imCherry) using the Cre-loxP system, whereas normal rats exhibited ubiquitous expression of eGFP but not mCherry in the whole body. Injection of adeno-associated virus serotype 9-Cre into the hippocampus and skeletal muscle resulted in mCherry expression in virus-infected cells. Cre-loxP-mediated mCherry expression was then evaluated by crossing Rosa26-imCherry rats with transgenic rats ubiquitously expressing CAG-Cre, heart-specific α-MHC-Cre transgenic rats and liver-specific Alb-Cre knockin rats. Finally, using the established system the expression pattern of Cre driven by two endogenous gene promoters (Wfs1-Cre knockin rat, FabP2-Cre knockin rat) was traced. In summary, we demonstrated excision of the loxP-flanked allele in Rosa26-imCherry rats via activation of mCherry expression in the presence of Cre recombinase. This newly established Rosa26-imCherry rat strain represents a useful tool to facilitate Cre-expression pattern determination and tracing experiments. © 2017 Federation of European Biochemical Societies.

  10. Methods to Regulate Unbundled Transmission and Distribution Business on Electricity Markets

    Energy Technology Data Exchange (ETDEWEB)

    Forsberg, Kaj; Fritz, Peter

    2003-11-01

    The regulation of distribution utilities is evolving from the traditional approach based on a cost of service or rate of return remuneration, to ways of regulation more specifically focused on providing incentives for improving efficiency, known as performance-based regulation or ratemaking. Modern regulation systems are also, to a higher degree than previously, intended to simulate competitive market conditions. The Market Design 2003-conference gathered people from 18 countries to discuss 'Methods to regulate unbundled transmission and distribution business on electricity markets'. Speakers from nine different countries and backgrounds (academics, industry and regulatory) presented their experiences and most recent works on how to make the regulation of unbundled distribution business as accurate as possible. This paper does not claim to be a fully representative summary of everything that was presented or discussed during the conference. Rather, it is a purposely restricted document where we focus on a few central themes and experiences from different countries.

  11. Electric Vehicle Based Battery Storages for Future Power System Regulation Services

    DEFF Research Database (Denmark)

    Pillai, Jayakrishnan Radhakrishna; Bak-Jensen, Birgitte

    2009-01-01

    supplying the reserve power requirements. This limited regulation services from conventional generators in the future power system calls for other new reserve power solutions like Electric Vehicle (EV) based battery storages. A generic aggregated EV based battery storage for long-term dynamic load frequency...

  12. Radical Prosumer Innovations in the Electricity Sector and the Impact on Prosumer Regulation

    Directory of Open Access Journals (Sweden)

    Saskia Lavrijssen

    2017-07-01

    Full Text Available The electricity sector is in a transition towards a Smart Energy System where the roles of private and institutional actors are evolving. This work deals with the influence of some technological innovations, enabling social innovations such peer to peer trading and the participation in local energy collectives, on the regulation of the rights and obligations of consumers and prosumers in the electricity sector. It identifies the main radical innovations in the electricity market and analyses the legal and related non-legal obstacles that may impede the empowerment of energy consumers and prosumers. Some recommendations are provided to ensure that consumers and prosumers are empowered and can benefit from these new technological and social innovations in the electricity market. The recommendations relate to an accurate definition of prosumers and active consumers, the integration of demand response, the evolving role of distribution network operators and the birth of peer-to-peer trading.

  13. CDC Vital Signs: Making Health Care Safer -- Stop Infections from Lethal CRE Germs Now

    Science.gov (United States)

    ... Program (EIP). Providing CRE outbreak support such as staff expertise, prevention guidelines, tools, and lab testing to states and facilities. Developing detection methods and prevention programs to control CRE. CDC's "Detect and Protect" effort supports regional ...

  14. Preserved dopaminergic homeostasis and dopamine-related behaviour in hemizygous TH-Cre mice

    DEFF Research Database (Denmark)

    Thomsen, Annika Højrup Runegaard; Jensen, Kathrine L; Fitzpatrick, Ciarán M

    2017-01-01

    assessment of the dopaminergic system in hemizygous tyrosine hydroxylase (TH)-Cre mice in comparison to wild-type (WT) controls. Our data show that TH-Cre mice display preserved dopaminergic homeostasis with unaltered levels of TH and dopamine as well as unaffected dopamine turnover in striatum. TH-Cre mice...

  15. Visualisation of Kiss1 Neurone Distribution Using a Kiss1-CRE Transgenic Mouse.

    Science.gov (United States)

    Yeo, S-H; Kyle, V; Morris, P G; Jackman, S; Sinnett-Smith, L C; Schacker, M; Chen, C; Colledge, W H

    2016-11-01

    Kisspeptin neuropeptides are encoded by the Kiss1 gene and play a critical role in the regulation of the mammalian reproductive axis. Kiss1 neurones are found in two locations in the rodent hypothalamus: one in the arcuate nucleus (ARC) and another in the RP3V region, which includes the anteroventral periventricular nucleus (AVPV). Detailed mapping of the fibre distribution of Kiss1 neurones will help with our understanding of the action of these neurones in other regions of the brain. We have generated a transgenic mouse in which the Kiss1 coding region is disrupted by a CRE-GFP transgene so that expression of the CRE recombinase protein is driven from the Kiss1 promoter. As expected, mutant mice of both sexes are sterile with hypogonadotrophic hypogonadism and do not show the normal rise in luteinising hormone after gonadectomy. Mutant female mice do not develop mature Graafian follicles or form corpora lutea consistent with ovulatory failure. Mutant male mice have low blood testosterone levels and impaired spermatogenesis beyond the meiosis stage. Breeding Kiss-CRE heterozygous mice with CRE-activated tdTomato reporter mice allows fluorescence visualisation of Kiss1 neurones in brain slices. Approximately 80-90% of tdTomato positive neurones in the ARC were co-labelled with kisspeptin and expression of tdTomato in the AVPV region was sexually dimorphic, with higher expression in females than males. A small number of tdTomato-labelled neurones was also found in other locations, including the lateral septum, the anterodorsal preoptic nucleus, the amygdala, the dorsomedial and ventromedial hypothalamic nuclei, the periaquaductal grey, and the mammillary nucleus. Three dimensional visualisation of Kiss1 neurones and fibres by CLARITY processing of whole brains showed an increase in ARC expression during puberty and higher numbers of Kiss1 neurones in the caudal region of the ARC compared to the rostral region. ARC Kiss1 neurones sent fibre projections to several

  16. Regulation of foreign trade with electric energy; Regulering av utenlandshandelen med kraft

    Energy Technology Data Exchange (ETDEWEB)

    Halseth, A.

    1995-04-28

    This report estimates the effects of regulated trade among the Scandinavian countries in a socio-economic perspective. Model simulations indicate that free trade is advantageous to Norway. In the short term foreign trade serves first of all to even out the effects of a variable supply of water to the hydroelectric power plants. In the long term trade with the other countries is connected primarily with peak load export/low load import. Free trade is advantageous to Denmark and Finland as well. In the short term free trade is essential for achieving a reasonably effective competition in the Swedish market. Simulations indicate, however, that in the long term Sweden may profit from trade regulations. 19 refs., 41 figs., 7 tabs.

  17. Research on Double Price Regulations and Peak Shaving Reserve Mechanism in Coal-Electricity Supply Chain

    Directory of Open Access Journals (Sweden)

    Hongjun Peng

    2013-01-01

    Full Text Available The game models were used to study the mechanism of coal-electricity price conflict under conditions of double price regulations of coal and electricity. Based on this, the peak shaving reserve mechanism was designed to probe into the countermeasures against the coal-electricity price conflicts. The study revealed that in the boom seasons of coal demand, the initiatives of the coal enterprises to supply thermal coal and the electricity enterprises to order thermal coal are reduced under conditions of double price regulations. However, under the circumstances of coal price marketization, in the boom seasons of coal demand the thermal coal price may go up obviously, the initiatives of the coal enterprises to supply thermal coal are increased, and meanwhile the initiatives of the power enterprises to order thermal coal are decreased dramatically. The transportation capacity constraint of coal supply leads to the evident decrease of the initiatives of coal enterprises for the thermal coal supply. The mechanism of peak shaving reserve of thermal coal may not only reduce the price of coal market but also increase the enthusiasm of the power enterprises to order more thermal coal and the initiatives of the coal enterprises to supply more thermal coal.

  18. The regulation of cell functions electrically using biodegradable polypyrrole-polylactide conductors.

    Science.gov (United States)

    Shi, Guixin; Zhang, Ze; Rouabhia, Mahmoud

    2008-10-01

    Electricity has a long history of being used as an alternative clinical treatment and as an effective approach to modifying cellular behaviours in vitro. It has been difficult, however, to take advantage of this modality in tissue generation because of the lack of suitable conductive, biocompatible scaffolding materials. In this study, in order to electrically regulate cell activities, a largely biodegradable conductor made of 5% conductive polypyrrole and 95% biodegradable poly(L-lactide) (PPy/PLLA) was prepared. Human cutaneous fibroblasts were cultured on the conductors in the presence or absence of a direct current (DC) electrical field (EF) of 50 mV/mm. The growth of the cells was characterized using fluorescent staining, SEM, and a MTT assay. The RNA expressions of interleukin-6 (IL-6) and interleukin-8 (IL-8) were assayed by RT-PCR. The amounts of IL-6 and IL-8 secreted by the fibroblasts were quantified by ELISA. The results showed that the PPy/PLLA conductors supported cell adhesion, spreading, and proliferation in both the presence and absence of the EF. Electrical stimulation (ES) applied through PPy/PLLA conductors dramatically enhanced cytokine secretion approximately 10-fold when compared to the non-ES controls. This effect lasted several days after the end of the ES. These findings highlight for the first time the possibility of a potent, effective approach to regulating tissue regeneration in conductive scaffolds through ES-modulated cytokine secretion, and to increasing cytokine productivity for biotechnological applications.

  19. Rapid systemic up-regulation of genes after heat-wounding and electrical stimulation

    Science.gov (United States)

    Davies, E.; Vian, A.; Vian, C.; Stankovic, B.

    1997-01-01

    When one leaf of a tomato plant is electrically-stimulated or heat-wounded, proteinase inhibitor genes are rapidly up-regulated in distant leaves. The identity of the systemic wound signal(s) is not yet known, but major candidates include hormones transmitted via the phloem or the xylem, the electrically-stimulated self-propagating electrical signal in the phloem (the action potential, AP), or the heat-wound-induced surge in hydraulic pressure in the xylem evoking a local change in membrane potential in adjacent living cells (the variation potential, VP). In order to discriminate between these signals we have adopted two approaches. The first approach involves applying stimuli that evoke known signals and determining whether these signals have similar effects on the "model" transcripts for proteinase inhibitors (pin) and calmodulin (cal). Here we show that a heat wound almost invariably evokes a VP, while an electrical stimulation occasionally evokes an AP, and both of these signals induce accumulation of transcripts encoding proteinase inhibitors. The second approach involves identifying the array of genes turned on by heat-wounding. To this end, we have constructed a subtractive library for heat-wounded tissue, isolated over 800 putatively up-regulated clones, and shown that all but two of the fifty that we have analyzed by Northern hybridization are, indeed, up-regulated. Here we show the early kinetics of up-regulation of three of these transcripts in the terminal (4th) leaf in response to heat-wounding the 3rd leaf, about 5 cm away. Even though these transcripts show somewhat different time courses of induction, with one peaking at 30 min, another at 15 min, and another at 5 min after flaming of a distant leaf, they all exhibit a similar pattern, i.e., a transient period of transcript accumulation preceding a period of transcript decrease, followed by a second period of transcript accumulation.

  20. 16th edition IEE wiring regulations design and verification of electrical installations

    CERN Document Server

    Scaddan, Brian

    1995-01-01

    This book builds on the basic knowledge and techniques covered in 16th Edition IEE Wiring Regulations Explained and Illustrated, providing the information and revision materials needed for the City & Guilds 2400 (Design, Erection and Verification ofElectrical Installations) exam. All Qualifying Managers will be required to gain this qualification, and Brian Scaddan's book is the ideal text for all students undertaking C&G 2400 courses.

  1. Electrical stimulation as a biomimicry tool for regulating muscle cell behavior.

    Science.gov (United States)

    Ahadian, Samad; Ostrovidov, Serge; Hosseini, Vahid; Kaji, Hirokazu; Ramalingam, Murugan; Bae, Hojae; Khademhosseini, Ali

    2013-01-01

    There is a growing need to understand muscle cell behaviors and to engineer muscle tissues to replace defective tissues in the body. Despite a long history of the clinical use of electric fields for muscle tissues in vivo, electrical stimulation (ES) has recently gained significant attention as a powerful tool for regulating muscle cell behaviors in vitro. ES aims to mimic the electrical environment of electroactive muscle cells (e.g., cardiac or skeletal muscle cells) by helping to regulate cell-cell and cell-extracellular matrix (ECM) interactions. As a result, it can be used to enhance the alignment and differentiation of skeletal or cardiac muscle cells and to aid in engineering of functional muscle tissues. Additionally, ES can be used to control and monitor force generation and electrophysiological activity of muscle tissues for bio-actuation and drug-screening applications in a simple, high-throughput, and reproducible manner. In this review paper, we briefly describe the importance of ES in regulating muscle cell behaviors in vitro, as well as the major challenges and prospective potential associated with ES in the context of muscle tissue engineering.

  2. CcpA represses the expression of the divergent cit operons of Enterococcus faecalis through multiple cre sites.

    Science.gov (United States)

    Suárez, Cristian A; Blancato, Víctor S; Poncet, Sandrine; Deutscher, Josef; Magni, Christian

    2011-10-11

    In Enterococcus faecalis the genes encoding the enzymes involved in citrate metabolism are organized in two divergent operons, citHO and oadHDB-citCDEFX-oadA-citMG (citCL locus). Expression of both operons is specifically activated by adding citrate to the medium. This activation is mediated by binding of the GntR-like transcriptional regulator (CitO) to the cis-acting sequences located in the cit intergenic region. Early studies indicated that citrate and glucose could not be co-metabolized suggesting some form of catabolite repression, however the molecular mechanism remained unknown. In this study, we observed that the citHO promoter is repressed in the presence of sugars transported by the Phosphoenolpyruvate:carbohydrate Phosphotranserase System (PTS sugars). This result strongly suggested that Carbon Catabolic Repression (CCR) impedes the expression of the activator CitO and the subsequent induction of the cit pathway. In fact, we demonstrate that CCR is acting on both promoters. It is partially relieved in a ccpA-deficient E. faecalis strain indicating that a CcpA-independent mechanism is also involved in regulation of the two operons. Furthermore, sequence analysis of the citH/oadH intergenic region revealed the presence of three putative catabolite responsive elements (cre). We found that they are all active and able to bind the CcpA/P-Ser-HPr complex, which downregulates the expression of the cit operons. Systematic mutation of the CcpA/P-Ser-HPr binding sites revealed that cre1 and cre2 contribute to citHO repression, while cre3 is involved in CCR of citCL. In conclusion, our study establishes that expression of the cit operons in E. faecalis is controlled by CCR via CcpA-dependent and -independent mechanisms.

  3. Electric drive vehicle systems : suggested changes to large truck and motorcoach regulations and inspection procedures : [technology brief].

    Science.gov (United States)

    2015-11-01

    Most commercial vehicles on the road today use : low-voltage electrical systems (12/24 volts direct : current). The current Federal Motor Carrier Safety : Regulations (FMCSRs), North American Standard : (NAS) inspection procedures, and out-of-service...

  4. Plug-in hybrid electric vehicles as a source of distributed frequency regulation

    Science.gov (United States)

    Mullen, Sara Kathryn

    The movement to transform the North American power grid into a smart grid may be accomplished by expanding integrated sensing, communications, and control technologies to include every part of the grid to the point of end-use. Plug-in hybrid electric vehicles (PHEV) provide an opportunity for small-scale distributed storage while they are plugged-in. With large numbers of PHEV and the communications and sensing associated with the smart grid, PHEV could provide ancillary services for the grid. Frequency regulation is an ideal service for PHEV because the duration of supply is short (order of minutes) and it is the highest priced ancillary service on the market offering greater financial returns for vehicle owners. Using Simulink a power system simulator modeling the IEEE 14 Bus System was combined with a model of PHEV charging and the controllers which facilitate vehicle-to-grid (V2G) regulation supply. The system includes a V2G controller for each vehicle which makes regulation supply decisions based on battery state, user preferences, and the recommended level of supply. A PHEV coordinator controller located higher in the system has access to reliable frequency measurements and can determine a suitable local automatic generation control (AGC) raise/lower signal for participating vehicles. A first step implementation of the V2G supply system where battery charging is modulated to provide regulation was developed. The system was simulated following a step change in loading using three scenarios: (1) Central generating units provide frequency regulation, (2) PHEV contribute to primary regulation analogous to generator speed governor control, and (3) PHEV contribute to primary and secondary regulation using an additional integral term in the PHEV control signal. In both cases the additional regulation provided by PHEV reduced the area control error (ACE) compared to the base case. Unique contributions resulting from this work include: (1) Studied PHEV energy systems

  5. Problems of standardizing and technical regulation in the electric power industry

    Science.gov (United States)

    Grabchak, E. P.

    2016-12-01

    A mandatory condition to ensure normal operation of a power system and efficiency in the sector is standardization and legal regulation of technological activities of electric power engineering entities and consumers. Compared to the times of USSR, the present-time technical guidance documents are not mandatory to follow in most cases, being of an advisory nature due to the lack of new ones. During the last five years, the industry has been showing a deterioration of the situation in terms of ensuring reliability and engineering controllability as a result of the dominant impact of short-term market stimuli and the differences in basic technological policies. In absence of clear requirements regarding the engineering aspects of such activities, production operation does not contribute to the preserving of technical integrity of the Russian power system, which leads to the loss of performance capability and controllability and causes disturbances in the power supply to consumers. The result of this problem is a high rate of accident incidence. The dynamics of accidents by the type of equipment is given, indicating a persisting trend of growth in the number of accidents, which are of a systematic nature. Several problematic aspects of engineering activities of electric power engineering entities, requiring standardization and legal regulation are pointed out: in the domestic power system, a large number of power electrotechnical and generating equipment operate along with systems of regulation, which do not comply with the principles and technical rules representing a framework where the Energy System of Russia is built and functioning

  6. A Nestin-cre transgenic mouse is insufficient for recombination in early embryonic neural progenitors

    Directory of Open Access Journals (Sweden)

    Huixuan Liang

    2012-09-01

    Nestin-cre transgenic mice have been widely used to direct recombination to neural stem cells (NSCs and intermediate neural progenitor cells (NPCs. Here we report that a readily utilized, and the only commercially available, Nestin-cre line is insufficient for directing recombination in early embryonic NSCs and NPCs. Analysis of recombination efficiency in multiple cre-dependent reporters and a genetic mosaic line revealed consistent temporal and spatial patterns of recombination in NSCs and NPCs. For comparison we utilized a knock-in Emx1cre line and found robust recombination in NSCs and NPCs in ventricular and subventricular zones of the cerebral cortices as early as embryonic day 12.5. In addition we found that the rate of Nestin-cre driven recombination only reaches sufficiently high levels in NSCs and NPCs during late embryonic and early postnatal periods. These findings are important when commercially available cre lines are considered for directing recombination to embryonic NSCs and NPCs.

  7. Tie-1-directed expression of Cre recombinase in endothelial cells of embryoid bodies and transgenic mice

    DEFF Research Database (Denmark)

    Gustafsson, E; Brakebusch, C; Hietanen, K

    2001-01-01

    Tissue-specific gene inactivation using the Cre-loxP system has become an important tool to unravel functions of genes when the conventional null mutation is lethal. We report here the generation of a transgenic mouse line expressing Cre recombinase in endothelial cells. In order to avoid...... the production and screening of multiple transgenic lines we used embryonic stem cell and embryoid body technology to identify recombinant embryonic stem cell clones with high, endothelial-specific Cre activity. One embryonic stem cell clone that showed high Cre activity in endothelial cells was used to generate...... germline chimeras. The in vivo efficiency and specificity of the transgenic Cre was analysed by intercrossing the tie-1-Cre line with the ROSA26R reporter mice. At initial stages of vascular formation (E8-9), LacZ staining was detected in almost all cells of the forming vasculature. Between E10 and birth...

  8. Generation of ER{alpha}-floxed and knockout mice using the Cre/LoxP system

    Energy Technology Data Exchange (ETDEWEB)

    Antonson, P., E-mail: per.antonson@ki.se [Department of Biosciences and Nutrition, Karolinska Institutet, Novum, SE-141 83 Huddinge (Sweden); Omoto, Y.; Humire, P. [Department of Biosciences and Nutrition, Karolinska Institutet, Novum, SE-141 83 Huddinge (Sweden); Gustafsson, J.-A. [Department of Biosciences and Nutrition, Karolinska Institutet, Novum, SE-141 83 Huddinge (Sweden); Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 (United States)

    2012-08-10

    Highlights: Black-Right-Pointing-Pointer ER{alpha} floxed and knockout mice were generated. Black-Right-Pointing-Pointer Disruption of the ER{alpha} gene results in sterility in both male and female mice. Black-Right-Pointing-Pointer ER{alpha}{sup -/-} mice have ovaries with hemorrhagic follicles and hypoplastic uterus. Black-Right-Pointing-Pointer Female ER{alpha}{sup -/-} mice develop obesity. -- Abstract: Estrogen receptor alpha (ER{alpha}) is a nuclear receptor that regulates a range of physiological processes in response to estrogens. In order to study its biological role, we generated a floxed ER{alpha} mouse line that can be used to knock out ER{alpha} in selected tissues by using the Cre/LoxP system. In this study, we established a new ER{alpha} knockout mouse line by crossing the floxed ER{alpha} mice with Cre deleter mice. Here we show that genetic disruption of the ER{alpha} gene in all tissues results in sterility in both male and female mice. Histological examination of uterus and ovaries revealed a dramatically atrophic uterus and hemorrhagic cysts in the ovary. These results suggest that infertility in female mice is the result of functional defects of the reproductive tract. Moreover, female knockout mice are hyperglycemic, develop obesity and at the age of 4 months the body weight of these mice was more than 20% higher compared to wild type littermates and this difference increased over time. Our results demonstrate that ER{alpha} is necessary for reproductive tract development and has important functions as a regulator of metabolism in females.

  9. A pink mouse reports the switch from red to green fluorescence upon Cre-mediated recombination

    OpenAIRE

    Hartwich Heiner; Satheesh Somisetty V; Nothwang Hans

    2012-01-01

    Abstract Background Targeted genetic modification in the mouse becomes increasingly important in biomedical and basic science. This goal is most often achieved by use of the Cre/loxP system and numerous Cre-driver mouse lines are currently generated. Their initial characterization requires reporter mouse lines to study the in vivo spatiotemporal activity of Cre. Findings Here, we report a dual fluorescence reporter mouse line, which switches expression from the red fluorescent protein mCherry...

  10. Regulating electricity demand peaks for home appliances using reversible fair scheduling

    DEFF Research Database (Denmark)

    Kardaras, Georgios; Rossello Busquet, Ana; Iversen, Villy Bæk

    2010-01-01

    of home appliances into priority classes and the definition of a maximum power consumption threshold which is not allowed to be exceeded during peak hours. According to the bandwidth demand and priority of each class, the reversible fair scheduling algorithm delays some of the appliances and prolongs......This paper describes a novel methodology for regulating electricity demand peaks for home appliances. To achieve this objective, we will make use of the reversible fair scheduling algorithm originally developed for telecommunication networks. The main concept behind this approach is the aggregation...

  11. THE STUDY OF TRANSIENT PROCESSES OF THE FREQUENCY-REGULATED SYNCHRONOUS ELECTRIC DRIVE

    Directory of Open Access Journals (Sweden)

    B. I. Firago

    2016-01-01

    Full Text Available In order to improve the power indices and to simplify the system of frequency speed control the scope of application of synchronous variable-frequency electric drives with independent frequency setting is being expanded. The synchronous motors with electromagnetic excitation and permanent magnet excitation in various industrial settings, including load-lifting machines and mechanisms, are used. As compared with the asynchronous frequency-regulated electric drives the synchronous ones have lower power loss, harder mechanical characteristic without feedback for speed and the simplest law of frequency control, i. e., a proportional one that, however, provides the maximum electromagnetic torque of the motor constant at all frequencies, due to the constant magnetic flux. The article concerns an analytical study of transient processes of synchronous electric drive with consideration of the influence of damping winding when the motor supply voltage frequency varies linearly during the transient time. As a result of the analysis the formulas have been obtained that make it possible to calculate the angular velocity of the rotor and the electromagnetic torque of the motor at start-up, braking, and impingement and discharge of loads, evaluating the quality of the transition process and tracking the maximum value of the electromagnetic torque, that must not exceed the permissible value. Validation of the developed technique of calculation of transient processes of synchronous electric drive has been obtained by comparison of calculations according to the formulas with the simulation results of the electric drive on the basis of the synchronous motor of the SD3 13-34-6 type (power of 500 kW and voltage of 6 kV.

  12. The Efficiency of the Regulation for Horizontal Mergers Among Electricity Distribution Operators in Norway

    DEFF Research Database (Denmark)

    Agrell, Per J.; Bogetoft, Peter; Gammeltvedt, Thor Erik

    2015-01-01

    Merger analysis is usually drawing on concepts derived from competitive horizontal markets, such as market concentration, predatory foreclosure and economies of scale in marketing. In evaluating horizontal mergers among energy network operators, regulators must use different tools to evaluate...... the attractiveness of mergers. Norway is one country having implemented an ex ante regulation for mergers among electricity distribution operators (DSOs). We investigate whether the actual mergers of Norwegian DSOs in the period 1995-2004 can be rationalized as cost saving exercises or whether they should...... be interpreted in other ways. We use Data Envelopment Analysis (DEA) to estimate the potential cost savings from mergers ex ante, and we decompose the gains into gains from learning best practice and from improving economies of scale and scope. Moreover, we compare the sources of post merger performance...

  13. Research on temperature control with numerical regulators in electric resistance furnaces with indirect heating

    Science.gov (United States)

    Diniş, C. M.; Popa, G. N.; Iagăr, A.

    2016-02-01

    The paper is an analysis of two-positions (hysteresis) regulators, self-tuned PID controller and PID controller for temperature control used for indirect heat resistance furnaces. For PID controller was used three methods of tuning: Ziegler-Nichols step response model, Cohen-Coon tuning rules and Ziegler-Nichols tuning rules. In experiments it used an electric furnace with indirect heating with active power of resistance of 1 kW/230V AC and a numerical temperature regulator AT-503 type (ANLY). It got a much better temperature control when using the Cohen-Coon tuning rules method than those of Ziegler-Nichols step response method and Ziegler-Nichols tuning rules method.

  14. A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans

    Science.gov (United States)

    Kimura, Yoshishige; Corcoran, Ethan E.; Eto, Koh; Gengyo-Ando, Keiko; Muramatsu, Masa-aki; Kobayashi, Ryoji; Freedman, Jonathan H.; Mitani, Shohei; Hagiwara, Masatoshi; Means, Anthony R.; Tokumitsu, Hiroshi

    2002-01-01

    Calcium (Ca2+) signals regulate a diverse set of cellular responses, from proliferation to muscular contraction and neuro-endocrine secretion. The ubiquitous Ca2+ sensor, calmodulin (CaM), translates changes in local intracellular Ca2+ concentrations into changes in enzyme activities. Among its targets, the Ca2+/CaM-dependent protein kinases I and IV (CaMKs) are capable of transducing intraneuronal signals, and these kinases are implicated in neuronal gene regulation that mediates synaptic plasticity in mammals. Recently, the cyclic AMP response element binding protein (CREB) has been proposed as a target for a CaMK cascade involving not only CaMKI or CaMKIV, but also an upstream kinase kinase that is also CaM regulated (CaMKK). Here, we report that all components of this pathway are coexpressed in head neurons of Caenorhabditis elegans. Utilizing a transgenic approach to visualize CREB-dependent transcription in vivo, we show that this CaMK cascade regulates CRE-mediated transcription in a subset of head neurons in living nematodes. PMID:12231504

  15. Improved α-Amylase Production by Dephosphorylation Mutation of CreD, an Arrestin-Like Protein Required for Glucose-Induced Endocytosis of Maltose Permease and Carbon Catabolite Derepression in Aspergillus oryzae.

    Science.gov (United States)

    Tanaka, Mizuki; Hiramoto, Tetsuya; Tada, Hinako; Shintani, Takahiro; Gomi, Katsuya

    2017-07-01

    Aspergillus oryzae produces copious amount of amylolytic enzymes, and MalP, a major maltose permease, is required for the expression of amylase-encoding genes. The expression of these genes is strongly repressed by carbon catabolite repression (CCR) in the presence of glucose. MalP is transported from the plasma membrane to the vacuole by endocytosis, which requires the homolog of E6-AP carboxyl terminus ubiquitin ligase HulA, an ortholog of yeast Rsp5. In yeast, arrestin-like proteins mediate endocytosis as adaptors of Rsp5 and transporters. In the present study, we examined the involvement of CreD, an arrestin-like protein, in glucose-induced MalP endocytosis and CCR of amylase-encoding genes. Deletion of creD inhibited the glucose-induced endocytosis of MalP, and CreD showed physical interaction with HulA. Phosphorylation of CreD was detected by Western blotting, and two serine residues were determined as the putative phosphorylation sites. However, the phosphorylation state of the serine residues did not regulate MalP endocytosis and its interaction with HulA. Although α-amylase production was significantly repressed by creD deletion, both phosphorylation and dephosphorylation mimics of CreD had a negligible effect on α-amylase activity. Interestingly, dephosphorylation of CreD was required for CCR relief of amylase genes that was triggered by disruption of the deubiquitinating enzyme-encoding gene creB The α-amylase activity of the creB mutant was 1.6-fold higher than that of the wild type, and the dephosphorylation mimic of CreD further improved the α-amylase activity by 2.6-fold. These results indicate that a combination of the dephosphorylation mutation of CreD and creB disruption increased the production of amylolytic enzymes in A. oryzae IMPORTANCE In eukaryotes, glucose induces carbon catabolite repression (CCR) and proteolytic degradation of plasma membrane transporters via endocytosis. Glucose-induced endocytosis of transporters is mediated by

  16. Lactation Defect in a Widely Used MMTV-Cre Transgenic Line of Mice

    Science.gov (United States)

    Yuan, Taichang; Wang, Yongping; Pao, Lily; Anderson, Steve M.; Gu, Haihua

    2011-01-01

    Background MMTV-Cre mouse lines have played important roles in our understanding about the functions of numerous genes in mouse mammary epithelial cells during mammary gland development and tumorigenesis. However, numerous studies have not included MMTV-Cre mice as controls, and many investigators have not indicated which of the different MMTV-Cre founder lines were used in their studies. Here, we describe a lactation defect that severely limits the use of one of the most commonly used MMTV-Cre founder lines. Methodology/Principal Findings To explore the role of protein tyrosine phosphatase Shp1 in mammary gland development, mice bearing the floxed Shp1 gene were crossed with MMTV-Cre mice and mammary gland development was examined by histological and biochemical techniques, while lactation competency was assessed by monitoring pup growth. Surprisingly, both the Shp1fl/+;MMTV-Cre and MMTV-Cre female mice displayed a severe lactation defect when compared to the Shp1 fl/+ control mice. Histological and biochemical analyses reveal that female mice expressing the MMTV-Cre transgene, either alone or in combination with floxed genes, exhibit defects in lobuloalveolar expansion, presence of large cytoplasmic lipid droplets in luminal alveolar epithelial cells postpartum, and precocious induction of involution. Using a PCR-based genotyping method, the three different founder lines can be distinguished, and we determined that the MMTV-Cre line A, the most widely used MMTV-Cre founder line, exhibits a profound lactation defect that limits its use in studies on mammary gland development. Conclusions/Significance The identification of a lactation defect in the MMTV-Cre line A mice indicates that investigators must use MMTV-Cre alone mice as control in studies that utilize Cre recombinase to excise genes of interest from mammary epithelial cells. Our results also suggest that previous results obtained in studies using the MMTV-Cre line A line should be re-evaluated if the

  17. State Performance-Based Regulation Using Multiyear Rate Plans for U.S. Electric Utilities

    Energy Technology Data Exchange (ETDEWEB)

    Lowry, Mark Newton [Pacific Economics Group Research LLC (United States); Makos, Matt [Pacific Economics Group Research LLC (United States); Deason, Jeff [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Schwartz, Lisa [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2017-07-31

    Electric utilities today must contain costs at a time when many need to modernize aging systems and all face major changes in technologies, customer preferences and competitive pressures.Most U.S. electric utility facilities are investor-owned, subject to rate and service regulation by state public utility commissions. Regulatory systems under which these utilities operate affect their performance and ability to meet these challenges. In this business environment, multiyear rate plans have some advantages over traditional rate regulation.The report focuses on key design issues and provides case studies of the multiyear rate plan approach, applicable to both vertically integrated and restructured states. Mark Newton Lowry and Matt Makos of Pacific Energy Group Research and Jeff Deason of Berkeley Lab authored the report; Lisa Schwartz, Berkeley Lab, was project manager and technical editor.The report is aimed primarily at state utility regulators and stakeholders in the state regulatory process. The multiyear rate approach also provides ideas on how to streamline oversight of public power utilities and rural electric cooperatives for their governing boards.Two key provisions of multiyear rate plans strengthen cost containment incentives and streamline regulation: 1. Reducing frequency of rate cases, typically to every four or five years 2. Using an attrition relief mechanism to escalate rates or revenue between rate cases to address cost pressures such as inflation and growth in number of customers, independently of the utility’s own cost Better utility performance can be achieved under well-designed multiyear rate plans while achieving lower regulatory costs. Benefits can be shared between utilities and their customers. But plans can be complex and involve significant changes in the regulatory system. Designing plans that stimulate utility performance without undue risk and share benefits fairly can be challenging.This report discusses the rationale for multiyear

  18. The homeobox transcription factor Even-skipped regulates acquisition of electrical properties in Drosophila neurons

    Directory of Open Access Journals (Sweden)

    Brand Andrea H

    2006-11-01

    Full Text Available Abstract Background While developmental processes such as axon pathfinding and synapse formation have been characterized in detail, comparatively less is known of the intrinsic developmental mechanisms that regulate transcription of ion channel genes in embryonic neurons. Early decisions, including motoneuron axon targeting, are orchestrated by a cohort of transcription factors that act together in a combinatorial manner. These transcription factors include Even-skipped (Eve, islet and Lim3. The perdurance of these factors in late embryonic neurons is, however, indicative that they might also regulate additional aspects of neuron development, including the acquisition of electrical properties. Results To test the hypothesis that a combinatorial code transcription factor is also able to influence the acquisition of electrical properties in embryonic neurons we utilized the molecular genetics of Drosophila to manipulate the expression of Eve in identified motoneurons. We show that increasing expression of this transcription factor, in two Eve-positive motoneurons (aCC and RP2, is indeed sufficient to affect the electrical properties of these neurons in early first instar larvae. Specifically, we observed a decrease in both the fast K+ conductance (IKfast and amplitude of quantal cholinergic synaptic input. We used charybdotoxin to pharmacologically separate the individual components of IKfast to show that increased Eve specifically down regulates the Slowpoke (a BK Ca2+-gated potassium channel, but not Shal, component of this current. Identification of target genes for Eve, using DNA adenine methyltransferase identification, revealed strong binding sites in slowpoke and nAcRα-96Aa (a nicotinic acetylcholine receptor subunit. Verification using real-time PCR shows that pan-neuronal expression of eve is sufficient to repress transcripts for both slo and nAcRα-96Aa. Conclusion Taken together, our findings demonstrate, for the first time, that Eve

  19. Th european market of the electric power; Le marche europeen de l'electricite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    This document presents the CRE (commission of the Electric power Control) progress report concerning the first july 2000 to the 30 june 2001. Three main subjects are discussed, illustrated by economic data and graphs: the electric power european market, the french market control and the CRE. A special interest is given to the deregulation of the market and its consequences. (A.L.B.)

  20. Tg(Th-CreFI172Gsat (Th-Cre defines neurons that are required for full hypercapnic and hypoxic reflexes

    Directory of Open Access Journals (Sweden)

    Jenny J. Sun

    2017-08-01

    Full Text Available The catecholaminergic (CA system has been implicated in many facets of breathing control and offers an important target to better comprehend the underlying etiologies of both developmental and adult respiratory pathophysiologies. Here, we used a noninvasive DREADD-based pharmacogenetic approach to acutely perturb Tg(Th-CreFI172Gsat (Th-Cre-defined neurons in awake and unrestrained mice in an attempt to characterize CA function in breathing. We report that clozapine-N-oxide (CNO-DREADD-mediated inhibition of Th-Cre-defined neurons results in blunted ventilatory responses under respiratory challenge. Under a hypercapnic challenge (5% CO2/21% O2/74% N2, perturbation of Th-Cre neurons results in reduced fR, and . Under a hypoxic challenge (10% O2/90% N2, we saw reduced fR, and , in addition to instability in both interbreath interval and tidal volume, resulting in a Cheyne-Stokes-like respiratory pattern. These findings demonstrate the necessity of Th-Cre-defined neurons for the hypercapnic and hypoxic ventilatory responses and breathing stability during hypoxia. However, given the expanded non-CA expression domains of the Tg(Th-CreFI172Gsat mouse line found in the brainstem, full phenotypic effect cannot be assigned solely to CA neurons. Nonetheless, this work identifies a key respiratory population that may lead to further insights into the circuitry that maintains respiratory stability in the face of homeostatic challenges.

  1. Generation of Shox2-Cre allele for tissue specific manipulation of genes in the developing heart, palate, and limb

    OpenAIRE

    Sun, Cheng; Zhang, Tao; Liu, Chao; Gu, Shuping; Chen, YiPing

    2013-01-01

    Shox2 is expressed in several developing organs in a tissue specific manner in both mice and humans, including the heart, palate, limb, and nervous system. To better understand the spatial and temporal expression patterns of Shox2 and to systematically dissect the genetic cascade regulated by Shox2, we created Shox2-LacZ and Shox2-Cre knock-in mouse lines. We show that the Shox2-LacZ allele expresses beta-galactosidase reporter gene in a fashion that recapitulates the endogenous Shox2 express...

  2. Cytokeratin 19 promoter directs the expression of Cre recombinase in various epithelia of transgenic mice.

    Science.gov (United States)

    Zhao, Gui-Feng; Zhao, Shuang; Liu, Jia-Jie; Wu, Ji-Cheng; He, Hao-Yu; Ding, Xiao-Qing; Yu, Xue-Wen; Huang, Ke-Qiang; Li, Zhi-Jie; Zheng, Hua-Chuan

    2017-03-14

    Cytokeratin 19 (K19) is expressed in various differentiated cells, including gastric, intestinal and bronchial epithelial cells, and liver duct cells. Here, we generated a transgenic mouse line, K19-Cre, in which the expression of Cre recombinase was controlled by the promoter of K19. To test the tissue distribution and excision activity of Cre recombinase, K19-Cre transgenic mice were bred with Rosa26 reporter strain and a mouse strain that carries PTEN conditional alleles (PTENLoxp/Loxp). At mRNA level, Cre was strongly expressed in the stomach, lung and intestine, while in stomach, lung, and liver at protein level. The immunoreactivity to Cre was strongly observed the cytoplasm of gastric, bronchial and intestinal epithelial cells. Cre activity was detectable in gastric, bronchial and intestinal epithelial cells, according to LacZ staining. In K19-Cre/PTEN Loxp/Loxp mice, PTEN was abrogated in stomach, intestine, lung, liver and breast, the former two of which were verified by in situ PCR. There appeared breast cancer with PTEN loss. These data suggest that K19 promoter may be a useful tool to study the pathophysiological functions of cytokeratin 19-positive cells, especially gastrointestinal epithelial cells. Cell specificity of neoplasia is not completely attributable to the cell-specific expression of oncogenes and cell-specific loss of tumor suppressor genes.

  3. Promiscuous recombination of LoxP alleles during gametogenesis in cornea Cre driver mice

    Science.gov (United States)

    Weng, Daniel Y.; Zhang, Yujin; Hayashi, Yasuhito; Kuan, Chia-Yi; Liu, Chia-Yang; Babcock, George; Weng, Wei-Lan; Schwemberger, Sandy

    2008-01-01

    Purpose To examine whether promiscuous Cre/LoxP recombination happens during gametogenesis in double transgenic mice carrying LoxP modified alleles and Cre transgene driven by tissue-specific promoter outside the gonads of adult mice. Methods Cre driver mice were crossbred with reporter mouse lines (e.g., ZEG and Rosa26R) to obtain Cre/ZEG and Cre/Rosa26R double transgenic mice. The frequency of promiscuous LoxP/Cre recombination was determined by the expression of second reporter genes in the offspring of double transgenic mice. Results The frequency of promiscuous LoxP/Cre recombination varied in different lines of Cre driver mice and in the sex of the same driver mice with higher penetrance in male than in female double transgenic mice. Polymerase chain reaction (PCR) and recombination analysis demonstrate that the recombination of floxed allele occurs during the transition from spermatogonia (diploid) to primary spermatocyte (tetraploid) in the testis. Thereby, target-floxed allele(s) may be ubiquitously ablated in experimental animals intended for tissue-specific gene deletion. Conclusions Gametogenesis-associated recombination should always be examined in tissue-specific gene ablation studies. PMID:18385792

  4. Effects of CreERT2, 4-OH Tamoxifen, and Gender on CFU-F Assays.

    Directory of Open Access Journals (Sweden)

    Sophie L McHaffie

    Full Text Available Gene function in stem cell maintenance is often tested by inducing deletion via the Cre-loxP system. However, controls for Cre and other variables are frequently not included. Here we show that when cultured in the presence of 4-OH tamoxifen, bone and marrow cells containing the CreERT2 construct have a reduced colony forming ability. Inactive CreERT2 recombinase, however, has the opposite effect. Young female marrow cells containing the inactive CreERT2 construct grew more colonies than cells lacking the construct altogether. Young female control marrow cells (i.e., negative for CreERT2 also produced significantly greater colony numbers when cultured with 4-OH tamoxifen, compared with the ethanol vehicle control. In conclusion, we report that the use of the Cre-loxP system is inadvisable in combination with CFU-F assays, and that appropriate controls should be in place to extend the future use of Cre-loxP in alternate assays.

  5. A CRE/AP-1-like motif is essential for induced syncytin-2 expression and fusion in human trophoblast-like model.

    Directory of Open Access Journals (Sweden)

    Chirine Toufaily

    Full Text Available Syncytin-2 is encoded by the envelope gene of Endogenous Retrovirus-FRD (ERVFRD-1 and plays a critical role in fusion of placental trophoblasts leading to the formation of the multinucleated syncytiotrophoblast. Its expression is consequently regulated in a strict manner. In the present study, we have identified a forskolin-responsive region located between positions -300 to -150 in the Syncytin-2 promoter region. This 150 bp region in the context of a minimal promoter mediated an 80-fold induction of promoter activity following forskolin stimulation. EMSA analyses with competition experiments with nuclear extracts from forskolin-stimulated BeWo cells demonstrated that the -211 to -177 region specifically bound two forskolin-induced complexes, one of them containing a CRE/AP-1-like motif. Site-directed mutagenesis of the CRE/AP-1 binding site in the context of the Syncytin-2 promoter or a heterologous promoter showed that this motif was mostly essential for forskolin-induced promoter activity. Transfection experiments with dominant negative mutants and constitutively activated CREB expression vectors in addition to Chromatin Immunoprecipitation suggested that a CREB family member, CREB2 was binding and acting through the CRE/AP-1 motif. We further demonstrated the binding of JunD to this same motif. Similar to forskolin and soluble cAMP, CREB2 and JunD overexpression induced Syncytin-2 promoter activity in a CRE/AP-1-dependent manner and Syncytin-2 expression. In addition, BeWo cell fusion was induced by both CREB2 and JunD overexpression, while being repressed following silencing of either gene. These results thereby demonstrate that induced expression of Syncytin-2 is highly dependent on the interaction of bZIP-containing transcription factors to a CRE/AP-1 motif and that this element is important for the regulation of Syncytin-2 expression, which results in the formation of the peripheral syncytiotrophoblast layer.

  6. Cell type-specific manipulation with GFP-dependent Cre recombinase.

    Science.gov (United States)

    Tang, Jonathan C Y; Rudolph, Stephanie; Dhande, Onkar S; Abraira, Victoria E; Choi, Seungwon; Lapan, Sylvain W; Drew, Iain R; Drokhlyansky, Eugene; Huberman, Andrew D; Regehr, Wade G; Cepko, Constance L

    2015-09-01

    There are many transgenic GFP reporter lines that allow the visualization of specific populations of cells. Using such lines for functional studies requires a method that transforms GFP into a molecule that enables genetic manipulation. We developed a method that exploits GFP for gene manipulation, Cre recombinase dependent on GFP (CRE-DOG), a split component system that uses GFP and its derivatives to directly induce Cre/loxP recombination. Using plasmid electroporation and AAV viral vectors, we delivered CRE-DOG to multiple GFP mouse lines, which led to effective recombination selectively in GFP-labeled cells. Furthermore, CRE-DOG enabled optogenetic control of these neurons. Beyond providing a new set of tools for manipulation of gene expression selectively in GFP(+) cells, we found that GFP can be used to reconstitute the activity of a protein not known to have a modular structure, suggesting that this strategy might be applicable to a wide range of proteins.

  7. Dual fluorescent reporter pig for Cre recombination: transgene placement at the ROSA26 locus.

    Directory of Open Access Journals (Sweden)

    Shun Li

    Full Text Available We are extending the Cre/loxP site-specific recombination system to pigs, focussing on conditional and tissue-specific expression of oncogenic mutations to model human cancers. Identifying the location, pattern and extent of Cre recombination in vivo is an important aspect of this technology. Here we report pigs with a dual fluorochrome cassette under the control of the strong CAG promoter that switches expression after Cre-recombination, from membrane-targeted tandem dimer Tomato to membrane-targeted green fluorescent protein. The reporter cassette was placed at the porcine ROSA26 locus by conventional gene targeting using primary mesenchymal stem cells, and animals generated by nuclear transfer. Gene targeting efficiency was high, and analysis of foetal organs and primary cells indicated that the reporter is highly expressed and functional. Cre reporter pigs will provide a multipurpose indicator of Cre recombinase activity, an important new tool for the rapidly expanding field of porcine genetic modification.

  8. Leptin regulation of inward membrane currents, electrical activity and LH release in isolated bovine gonadotropes.

    Science.gov (United States)

    Domínguez-Mancera, Belisario; Barrientos-Morales, Manuel; Cervantes-Acosta, Patricia; Hernández-Beltrán, Antonio; Rodríguez-Andrade, Araceli; González-Ramírez, Ricardo; Monjaraz, Eduardo; Felix, Ricardo

    2017-09-09

    Leptin, a peptide hormone produced by adipocytes, is recognized as one of the signals involved in the onset of reproductive activity. The leptin receptor has been found in hypothalamic neurons and pituitary gonadotropes, suggesting that the hormone may act at both sites to stimulate the secretion of GnRH and consequently, FSH and LH. In response to a stimulus such as a hypothalamic secretagogue, gonadotropes respond with changes in electrical activity, intracellular Ca(2+) and hormone release. The main aim of this report was to investigate whether leptin promotes a change in the electrical and secretory activities of bovine gonadotropes. After 48 h of treatment with leptin (10 nM) significant changes in the action potential properties were observed in gonadotropes, which included an increase in amplitude, time-to-pike and post-hyperpolarization, as well as a decrease in firing threshold. Likewise, leptin induced a significant (∼1.3-fold) up-regulation of voltage-gated Na(+) channel current density, and a selective increase (∼2.1-fold) in Ca(2+) current density through high voltage-activated channels. Consistent with this, leptin enhanced GnRH-induced secretion of LH measured by ELISA. We suggest that leptin enhances membrane expression of voltage-gated Na(+) and Ca(2+) channels, which results in a modulation of the action potential properties and an increase in hormone release from gonadotropes. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. French wholesale electricity and gas markets in 2007. Monitoring report; Le fonctionnement des marches de gros francais de l'electricite et du gaz naturel en 2007. Rapport de surveillance

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-12-15

    be improved at this stage. Published information on availability forecasts of power generation facilities were insufficient. Finally, CRE has established that the limited development of competition on the retail market and the implementation of the transitional regulated tariff for balancing markets (TaRTAM) have curbed the increase of the wholesale electricity market's activity. Considering these findings, CRE will carry out audits on the valuation methods used by EDF for its nuclear and hydraulic generation. Based on a public consultation, CRE will also formulate recommendations on generation transparency. On the gas wholesale market, CRE observes that the activity on the wholesale market has grown all along 2007 but remained focused on short-term trading. In particular, little public information was available on the market because most of the deals were concluded without intermediary. Moreover, CRE notes that the supplying conditions of suppliers who are not former incumbents in Europe were not satisfactory. These suppliers had to acquire most of their gas on the French market. In order to improve the transparency of the gas wholesale market, CRE will publish aggregated and anonymous information on the bilateral market according to information on volumes that will be collected from market participants.

  10. Generation of a transgenic mouse for colorectal cancer research with intestinal cre expression limited to the large intestine

    National Research Council Canada - National Science Library

    Xue, Yingben; Johnson, Robert; Desmet, Marsha; Snyder, Paul W; Fleet, James C

    2010-01-01

    .... We sought to create a transgenic mouse with Cre recombinase (Cre) expression limited to the epithelial cells of the large intestine and used this model to study colon cancer driven by adenomatosis polyposis coli (APC) gene inactivation...

  11. GFAP-Cre-mediated transgenic activation of Bmi1 results in pituitary tumors.

    Directory of Open Access Journals (Sweden)

    Bart A Westerman

    Full Text Available Bmi1 is a member of the polycomb repressive complex 1 and plays different roles during embryonic development, depending on the developmental context. Bmi1 over expression is observed in many types of cancer, including tumors of astroglial and neural origin. Although genetic depletion of Bmi1 has been described to result in tumor inhibitory effects partly through INK4A/Arf mediated senescence and apoptosis and also through INK4A/Arf independent effects, it has not been proven that Bmi1 can be causally involved in the formation of these tumors. To see whether this is the case, we developed two conditional Bmi1 transgenic models that were crossed with GFAP-Cre mice to activate transgenic expression in neural and glial lineages. We show here that these mice generate intermediate and anterior lobe pituitary tumors that are positive for ACTH and beta-endorphin. Combined transgenic expression of Bmi1 together with conditional loss of Rb resulted in pituitary tumors but was insufficient to induce medulloblastoma therefore indicating that the oncogenic function of Bmi1 depends on regulation of p16(INK4A/Rb rather than on regulation of p19(ARF/p53. Human pituitary adenomas show Bmi1 overexpression in over 50% of the cases, which indicates that Bmi1 could be causally involved in formation of these tumors similarly as in our mouse model.

  12. The Pax3-Cre transgene exhibits a rostrocaudal gradient of expression in the skeletal muscle lineage.

    Science.gov (United States)

    Jarad, George; Miner, Jeffrey H

    2009-01-01

    Pax3-Cre (P3Pro-Cre) transgenic mice have been used for conditional gene deletion and/or lineage tracing in derivatives of neural crest, neural tube, metanephric mesenchyme, and ureteric mesenchyme. However, the extent of its expression in skeletal muscle has not been reported. We investigated the expression of P3Pro-Cre in the skeletal muscle lineage using the R26R reporter and found an unexpected rostrocaudal gradient of expression. By X-gal staining, head, neck, forelimb, diaphragm, and most of the chest wall muscles did not show evidence of Cre expression, whereas all muscle groups posterior of the diaphragm stained blue. Intercostal muscles exhibited a rostrocaudal gradient of staining. The consistency of this expression pattern was demonstrated by using P3Pro-Cre to mutate a conditional dystroglycan allele. The result was loss of dystroglycan from caudal muscles, which exhibited the histological signs of muscle fiber injury and regeneration characteristic of muscular dystrophy. The lack of dystroglycan in regenerating myofibers suggests that the P3Pro-Cre transgene is active in satellite cells and/or in their precursors. In contrast, rostral muscles, including feeding and breathing muscles, maintained dystroglycan expression and were spared from disease. Accordingly, the mutants were viable for over a year. Its unique gradient of activity makes the P3Pro-Cre transgene a previously unappreciated yet powerful tool for manipulating gene expression in skeletal muscle and its precursors.

  13. Somatostatin-IRES-Cre Mice: Between Knockout and Wild-Type?

    Directory of Open Access Journals (Sweden)

    Cécile Viollet

    2017-06-01

    Full Text Available The neuropeptide somatostatin (SOM is widely expressed in rodent brain and somatostatin-IRES-Cre (SOM-cre mouse strains are increasingly used to unravel the physiology of SOM-containing neurons. However, while knock-in targeting strategy greatly improves Cre-Lox system accuracy, recent reports have shown that genomic insertion of Cre construct per se can markedly affect physiological function. We show that Cre transgene insertion into the 3′UTR of the somatostatin gene leads to the selective and massive depletion of endogenous SOM in all tested brain regions. It also strongly impacts SOM-related neuroendocrine responses in a similar manner to what has been reported for SST KO mice: increased corticosterone levels after 30-min restraint stress, decreased amplitude and regularity of ultradian growth hormone secretory patterns accompanied by changes in sexually dimorphic liver gene expression (serpina1, Cyp2b9, Cyp2a4, Cyp2d9, and Cyp7b1. In addition to demonstrating the need for examination of the consequences of Cre transgenesis, these results also reveal how this SOM-cre strain may be a useful tool in studying the functional consequences of moderate to low SOM levels as reported in neurological and psychiatric disorders.

  14. Spinal Hb9::Cre-derived excitatory interneurons contribute to rhythm generation in the mouse.

    Science.gov (United States)

    Caldeira, Vanessa; Dougherty, Kimberly J; Borgius, Lotta; Kiehn, Ole

    2017-01-27

    Rhythm generating neurons are thought to be ipsilaterally-projecting excitatory neurons in the thoracolumbar mammalian spinal cord. Recently, a subset of Shox2 interneurons (Shox2 non-V2a INs) was found to fulfill these criteria and make up a fraction of the rhythm-generating population. Here we use Hb9::Cre mice to genetically manipulate Hb9::Cre-derived excitatory interneurons (INs) in order to determine the role of these INs in rhythm generation. We demonstrate that this line captures a consistent population of spinal INs which is mixed with respect to neurotransmitter phenotype and progenitor domain, but does not overlap with the Shox2 non-V2a population. We also show that Hb9::Cre-derived INs include the comparatively small medial population of INs which continues to express Hb9 postnatally. When excitatory neurotransmission is selectively blocked by deleting Vglut2 from Hb9::Cre-derived INs, there is no difference in left-right and/or flexor-extensor phasing between these cords and controls, suggesting that excitatory Hb9::Cre-derived INs do not affect pattern generation. In contrast, the frequencies of locomotor activity are significantly lower in cords from Hb9::Cre-Vglut2(Δ/Δ) mice than in cords from controls. Collectively, our findings indicate that excitatory Hb9::Cre-derived INs constitute a distinct population of neurons that participates in the rhythm generating kernel for spinal locomotion.

  15. Endogenous n-3 Polyunsaturated Fatty Acids Delay Progression of Pancreatic Ductal Adenocarcinoma in Fat-1-p48Cre/+-LSL-KrasG12D/+ Mice

    Directory of Open Access Journals (Sweden)

    Altaf Mohammed

    2012-12-01

    Full Text Available Preclinical studies suggest that diets rich in omega-3 polyunsaturated fatty acids (n-3 PUFAs may be beneficial for prevention of pancreatic cancer. Nutritional intervention studies are often complex, and there is no clear evidence, without potential confounding factors, on whether conversion of n-6 PUFAs to n-3 PUFAs in pancreatic tissues would provide protection. Experiments were designed using n-3 fatty acid desaturase (Fat-1 transgenic mice, which can convert n-6 PUFA to n-3 FAs endogenously, to determine the impact of n-3 PUFAs on pancreatic intraepithelial neoplasms (PanINs and their progression to pancreatic ductal adenocarcinoma (PDAC. Six-weekold female p48Cre/+-LSL-KrasG12D/+ andcompoundFat-1-p48Cre/+-LSL-KrasG12D/+ mice were fed (AIN-76A diets containing 10% safflower oil for 35 weeks. Pancreata were evaluated histopathologically for PanINs and PDAC. Results showed a dramatic reduction in incidence of PDAC (84%; P 85%; P < .05–0.01 in pancreas of compound transgenic mice than in those of p48Cre/+-LSL-KrasG12D/+ mice. Molecular analysis of the pancreas showed a significant down-regulation of proliferating cell nuclear antigen, cyclooxygenase-2, 5-lipoxygenase (5-LOX, 5-LOX-activating protein, Bcl-2, and cyclin D1 expression levels in Fat-1-p48Cre/+-LSL-KrasG12D/+ mice compared to p48Cre/+-LSL-KrasG12D/+ mice. These data highlight the promise of dietary n-3 FAs for chemoprevention of pancreatic cancer in high-risk individuals.

  16. Physiological characterisation of recombinant Aspergillus nidulans strains with different creA genotypes expressing A-oryzae alpha-amylase

    DEFF Research Database (Denmark)

    Agger, Teit; Petersen, J.B.; O'Connor, S.M.

    2002-01-01

    The physiology of three strains of Aspergillus nidulans was examined-a creA deletion strain, a wild type creA genotype and a strain containing extra copies of the creA gene, all producing Aspergillus oryzae alpha-amylase. The strains were cultured in batch and continuous cultivations...

  17. Novel migrating mouse neural crest cell assay system utilizing P0-Cre/EGFP fluorescent time-lapse imaging

    Directory of Open Access Journals (Sweden)

    Kawakami Minoru

    2011-11-01

    Full Text Available Abstract Background Neural crest cells (NCCs are embryonic, multipotent stem cells. Their long-range and precision-guided migration is one of their most striking characteristics. We previously reported that P0-Cre/CAG-CAT-lacZ double-transgenic mice showed significant lacZ expression in tissues derived from NCCs. Results In this study, by embedding a P0-Cre/CAG-CAT-EGFP embryo at E9.5 in collagen gel inside a culture glass slide, we were able to keep the embryo developing ex vivo for more than 24 hours; this development was with enough NCC fluorescent signal intensity to enable single-cell resolution analysis, with the accompanying NCC migration potential intact and with the appropriate NCC response to the extracellular signal maintained. By implantation of beads with absorbed platelet-derived growth factor-AA (PDGF-AA, we demonstrated that PDGF-AA acts as an NCC-attractant in embryos. We also performed assays with NCCs isolated from P0-Cre/CAG-CAT-EGFP embryos on culture plates. The neuromediator 5-hydroxytryptamine (5-HT has been known to regulate NCC migration. We newly demonstrated that dopamine, in addition to 5-HT, stimulated NCC migration in vitro. Two NCC populations, with different axial levels of origins, showed unique distribution patterns regarding migration velocity and different dose-response patterns to both 5-HT and dopamine. Conclusions Although avian species predominated over the other species in the NCC study, our novel system should enable us to use mice to assay many different aspects of NCCs in embryos or on culture plates, such as migration, division, differentiation, and apoptosis.

  18. Investigating Relationships among Pre-Service Science Teachers' Conceptual Knowledge of Electric Current, Motivational Beliefs and Self-Regulation

    Science.gov (United States)

    Inaltun, Hüseyin; Ates, Salih

    2015-01-01

    The purpose of this study is to examine relationships among pre-service science teachers' conceptual knowledge of electric current, motivational beliefs, and self-regulation. One hundred and twenty-seven students (female = 107, male = 20) enrolled in the science education program of a public university in Ankara participated the study. A concept…

  19. Shut-Off Valves of Electric Drive with Thyristor Voltage Regulators and Software Implementation of the Indirect Determination of Moment

    Directory of Open Access Journals (Sweden)

    Vlasov Anton I.

    2017-01-01

    Full Text Available In this paper we consider the shut-off valve of electric drive with thyristor voltage regulator and microprocessor control which ensures a constant indirect control of torque value on the electric drive as well as its limitation in the case of the creation of an emergency on the main oil pipeline. It was found that the use of the integrated microprocessor control systems with software implementation calculating of given torque in the system of induction motor - thyristor voltage regulator, allows you to get the best performance of electric drive shut-off valve with the restrictions on the maximum current, torque whith deviations of voltage parameters and improve the safety of the process of oil pumping.

  20. Electricity and gas market observatory. 3. quarter 2006; Observatoire des marches de l'electricite et du gaz. 3. trimestre 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    Since July 2004, all electricity and gas consumers can be eligible according to their consumption site, as long as all or part of the electricity or gas consumed is designed for non-residential use. The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). It completes the information already published by CRE: practical information for eligible customers: consumer guide, list of suppliers, communications regarding markets running, CRE annual activity report. (author)

  1. Electricity and gas market observatory. 2. quarter 2006; Observatoire des marches de l'electricite et du gaz. 2. trimestre 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    Since July 2004, all electricity and gas consumers can be eligible according to their consumption site, as long as all or part of the electricity or gas consumed is designed for non-residential use. The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). It completes the information already published by CRE: practical information for eligible customers: consumer guide, list of suppliers, communications regarding markets running, CRE annual activity report. (author)

  2. Electricity and gas market observatory. 1. quarter 2006; Observatoire des marches de l'electricite et du gaz. 1. trimestre 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    Since July 2004, all electricity and gas consumers can be eligible according to their consumption site, as long as all or part of the electricity or gas consumed is designed for non-residential use. The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). It completes the information already published by CRE: practical information for eligible customers: consumer guide, list of suppliers, communications regarding markets running, CRE annual activity report. (author)

  3. Observatory of the electric power and gas markets. 16 February 2005; Observatoire des marches de l'electricite et du gaz. 16 fevrier 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-02-15

    Since July 2004, all electricity and gas consumers can be eligible according to their consumption site, as long as all or part of the electricity or gas consumed is designed for non-residential use. The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). It completes the information already published by CRE: practical information for eligible customers: consumer guide, list of suppliers, communications regarding markets running, CRE annual activity report. (author)

  4. Electricity and gas market observatory. 1. 2005 quarter; Observatoire des marches de l'electricite et du gaz. 1. trimestre 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    Since July 2004, all electricity and gas consumers can be eligible according to their consumption site, as long as all or part of the electricity or gas consumed is designed for non-residential use. The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). It completes the information already published by CRE: practical information for eligible customers: consumer guide, list of suppliers, communications regarding markets running, CRE annual activity report. (author)

  5. Electricity and gas market observatory. 4. 2005 quarter; Observatoire des marches de l'electricite et du gaz. 4. trimestre 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    Since July 2004, all electricity and gas consumers can be eligible according to their consumption site, as long as all or part of the electricity or gas consumed is designed for non-residential use. The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). It completes the information already published by CRE: practical information for eligible customers: consumer guide, list of suppliers, communications regarding markets running, CRE annual activity report. (author)

  6. Electricity and gas market observatory. 3. 2005 quarter; Observatoire des marches de l'electricite et du gaz. 3. trimestre 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    Since July 2004, all electricity and gas consumers can be eligible according to their consumption site, as long as all or part of the electricity or gas consumed is designed for non-residential use. The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). It completes the information already published by CRE: practical information for eligible customers: consumer guide, list of suppliers, communications regarding markets running, CRE annual activity report. (author)

  7. Electricity and gas market observatory. 2. 2005 quarter; Observatoire des marches de l'electricite et du gaz. 2. trimestre 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    Since July 2004, all electricity and gas consumers can be eligible according to their consumption site, as long as all or part of the electricity or gas consumed is designed for non-residential use. The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). It completes the information already published by CRE: practical information for eligible customers: consumer guide, list of suppliers, communications regarding markets running, CRE annual activity report. (author)

  8. DC microgrids with energy storage systems and demand response for providing support to frequency regulation of electrical power systems

    DEFF Research Database (Denmark)

    Basic, Hrvoje; Dragicevic, Tomislav; Pandzic, Hrvoje

    2017-01-01

    Frequency regulation of electric power systems efficiency depends on response time and on power reserves for frequency regulation. As integration of non-dispatchable renewable generation in the power system results with increased need for power reserves from fast responding power units, the idea...... of using aggregated DC microgrids in frequency regulation is presented. Model proposed in this work is based on using battery energy storage, combined with demand response for achieving efficient usage of battery energy storage. It is shown that large number of DC microgrids can provide sufficient....

  9. RoHS regulated substances in mixed plastics from waste electrical and electronic equipment.

    Science.gov (United States)

    Wäger, Patrick A; Schluep, Mathias; Müller, Esther; Gloor, Rolf

    2012-01-17

    The disposal and recovery of plastics from waste electrical and electronic equipment (WEEE) are of considerable importance, both from an environmental and an economic perspective. This paper presents the results of a study investigating current concentrations of hazardous substances in mixed plastics from WEEE and their implications for an environmentally sound recovery. The study included 53 sampling campaigns for mixed plastics from WEEE. The samples were analyzed with regard to heavy metals (cadmium, chromium, mercury, and lead) and flame retardants (PentaBDE, OctaBDE, DecaBDE, DecaBB) regulated in the RoHS Directive. Besides these substances, other brominated flame retardants known to occur in electronics (HBCD, TBBPA) as well as the total bromine and phosphorus contents were considered. Results show that no mixed plastics fraction from WEEE is completely free from substances regulated in the RoHS Directive. The lowest number and average concentrations were found in flat screen monitors. The highest concentrations were found in mixed plastics from CRT monitors and TVs. Mixed plastics fractions with high average concentrations of heavy metals originate from the treatment of small household appliances (cadmium), ICT equipment (lead), and consumer equipment (lead). Mixed plastics fractions with high average concentrations of brominated flame retardants mainly originate from the treatment of small household appliances for high temperature applications (DecaBDE), CRT monitors (OctaBDE and DecaBDE) and consumer equipment (DecaBDE), in particular CRT TVs (DecaBDE). To avoid a dissipation of hazardous substances into plastics and the environment, it is recommended that mixed plastics from WEEE are subject to a strict quality management.

  10. Concept and regulation of the reliability in electrical systems; Marco conceptual y regulacion de la confiabilidad en sistemas electricos

    Energy Technology Data Exchange (ETDEWEB)

    Aguero Vargas, Francisco; Salazar Jaque, Daniel [Comision Nacional de Energia, Santiago (Chile)]. E-mails: faguero@cne.cl; dsalazar@cne.cl

    2004-07-01

    In current electric power regulating in Chile, the emphasis has been placed on responding to economic needs of the electricity market, neglecting aspects of the operation of the electrical systems, which are presented in the conceptual framework proposed for the reliability, through the inclusion of complementary services and revenue from capacity the legislation of the Chilean electricity sector. Initially this article analyse the conflicts, product of the absence of legislation according to development that had the electricity industry in Chile. After then examines the legal background that gave rise to the bill, with relevant cases in comparative law. Finally, the milestones are traveled in the handling of bill amending the regime of Chilean electricity sector. The conclusion are presented the perspectives and imminent changes to the electricity market in Chile. That is, new role for consumers from new technologies; suitable incentives and opportunities to participate in new forms of market; changes in the design of the agency responsible for coordinating the operation; creating of a framework explicit for the safety and quality of service through the Code Red. (author)

  11. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain

    OpenAIRE

    Deverman, Benjamin E.; Pravdo, Piers L.; Simpson, Bryan P.; Kumar, Sripriya Ravindra; Chan, Ken Y.; Banerjee, Abhik; Wu, Wei-Li; Yang, Bin; Huber, Nina; Pasca, Sergiu P.; Gradinaru, Viviana

    2016-01-01

    Recombinant adeno-associated viruses (rAAVs) are commonly used vehicles for in vivo gene transfer 1-6 . However, the tropism repertoire of naturally occurring AAVs is limited, prompting a search for novel AAV capsids with desired characteristics 7-13 . Here we describe a capsid selection method, called Cre-recombination-based AAV targeted evolution (CREATE), that enables the development of AAV capsids that more efficiently transduce defined Cre-expressing cell populations in vivo. We use CREA...

  12. Acceleration Slip Regulation Strategy for Distributed Drive Electric Vehicles with Independent Front Axle Drive Motors

    Directory of Open Access Journals (Sweden)

    Lingfei Wu

    2015-05-01

    Full Text Available This paper presents an acceleration slip regulation strategy for distributed drive electric vehicles with two motors on the front axle. The tasks of the strategy include controlling the slip ratio to make full use of the road grip and controlling the yaw rate to eliminate the lateral movement due to the difference between motor torques. The rate of the slip ratio change can be controlled by controlling the motor torque, so that the slip ratio can be controlled by applying a proportional-integral control strategy to control the rate of the slip ratio change. The yaw rate can be controlled to almost zero by applying torque compensation based on yaw rate feedback. A coordination control strategy for the slip ratio control and yaw rate control is proposed based on analysis of the priorities and features of the two control processes. Simulations were carried out using MATLAB/Simulink, and experiments were performed on a hardware-in-loop test bench with actual motors. The results of the simulations and experiments showed that the proposed strategy could improve the longitudinal driving performance and straight line driving stability of the vehicle.

  13. Cardiac fibrosis in mice expressing an inducible myocardial-specific Cre driver

    Directory of Open Access Journals (Sweden)

    Jonas Lexow

    2013-11-01

    Tamoxifen-inducible Cre-mediated manipulation of animal genomes has achieved wide acceptance over the last decade, with numerous important studies heavily relying on this technique. Recently, a number of groups have reported transient complications of using this protocol in the heart. In the present study we observed a previously unreported focal fibrosis and depressed left-ventricular function in tamoxifen-treated αMHC-MerCreMer-positive animals in a Tβ4shRNAflox × αMHC-MerCreMer cross at 6–7 weeks following standard tamoxifen treatment, regardless of the presence of the floxed transgene. The phenotype was reproduced by treating mice from the original αMHC-MerCreMer strain with tamoxifen. In the acute phase after tamoxifen treatment, cell infiltration into the myocardium was accompanied by increased expression of pro-inflammatory cytokines (IL-1β, IL-6, TNFα, IFNγ, Ccl2 and markers of hypertrophy (ANF, BNP, Col3a1. These observations highlight the requirement for including tamoxifen-treated MerCreMer littermate controls to avert misinterpretation of conditional mutant phenotypes. A survey of the field as well as the protocols presented here suggests that controlling the parameters of tamoxifen delivery is important in avoiding the chronic MerCreMer-mediated cardiac phenotype reported here.

  14. Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivo

    Directory of Open Access Journals (Sweden)

    Edenhofer Frank

    2009-09-01

    Full Text Available Abstract Background Cre recombinase is a common reagent used for the in vivo on/off switching of the expression of target genes flanked by loxP sites. In particular, recombinant TAT-Cre fusion constructs purified from bacteria have been used to promote the cell uptake of the enzyme. However, the recovery of active TAT-Cre remains a demanding process and its specific activity varies significantly among batches, making difficult data comparison. Results We noticed a strong correlation between recombinase activity and enzyme monodispersity. The existence of such correlation enabled us to indirectly monitor the TAT-Cre recombinase activity during the multi-step purification process by measuring its monodispersity, a parameter detectable by means of a spectrofluorimetric assay that allows the calculation of the Aggregation Index (AI in an easy and rapid way. AI values were recorded after each purification passage to identify the critical steps and to choose optimal alternatives for chromatographic conditions, desalting procedures, and protocols for bacterial endotoxin removal. Furthermore, the effect of metal ions and temperature on TAT-Cre aggregation and inactivation was characterized in vitro. Finally, we optimized the enzyme delivery protocol in vivo by following the accumulation tuning of the reporter protein β-catenin. Conclusion A rational purification protocol for TAT-Cre has been developed by choosing the options that minimize the enzyme aggregation. Our data suggest that AI measurement should support the optimization of any protocol aiming at the recovery of monodispersed protein.

  15. Cre-loxP-Mediated Recombination: General Principles and Experimental Considerations.

    Science.gov (United States)

    McLellan, Micheal A; Rosenthal, Nadia A; Pinto, Alexander R

    2017-03-02

    The cre-loxP-mediated recombination system (the "cre-loxP system") is an integral experimental tool for mammalian genetics and cell biology. Use of the system has greatly expanded our ability to precisely interrogate gene function in the mouse, providing both spatial and temporal control of gene expression. This has been largely due to the simplicity of its use and its adaptability to address diverse biological questions. While the use of the cre-loxP system is becoming increasingly widespread, in particular because of growing availability of conditional mouse mutants, many considerations need to be taken into account when utilizing the cre-loxP system. This review provides an overview of the cre-loxP system and its various permutations. It addresses the limitations of cre-loxP technology and related considerations for experimental design, and it discusses alternative strategies for site-specific genetic recombination and integration. © 2017 by John Wiley & Sons, Inc. Copyright © 2017 John Wiley & Sons, Inc.

  16. Report of the French Energy Regulator on electricity interconnection management and use in 2006; Rapport de la Commission de Regulation de l'Energie sur la gestion et l'utilisation des interconnexions electriques en 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report aims at, first, evaluating the congestion management methods in force at interconnected power systems managed by the French transmission system operator RTE as required by article 1.10 of the guidelines of 1228/2003 European regulation which foresees that: 'the national regulation authorities regularly evaluates the congestion management methods, in particular by controlling the respect of rules and principles established by the European regulation, and of trends, modalities and conditions fixed by the regulation authorities according to the above principles and rules'. It aims also at sharing the opinions of the commission of energy regulation (CRE) with other European regulating authorities and with all stakeholders involved in the domestic power market, about the management and use of European power interconnections in 2006. Content: 1 - 2005-2006, the turning point for congestion management; 2 - consequences of 2055-2006 changes in interconnections with Germany, Belgium, Spain and Italy; 3 - further improvements still required: allocation system, capacity bids, short-term trades, capacity management in compliance with European law; 4 - conclusion: an indispensable regional approach. (J.S.)

  17. Structural and functional characterization of the coxsackievirus B3 CRE(2C): role of CRE(2C) in negative- and positive-strand RNA synthesis.

    NARCIS (Netherlands)

    Ooij, M.J. van; Vogt, D.A.; Paul, A.; Castro, C.; Kuijpers, J.M.; Kuppeveld, F.J.M. van; Cameron, C.E.; Wimmer, E.; Andino, R.; Melchers, W.J.G.

    2006-01-01

    A stem-loop element located within the 2C-coding region of the coxsackievirus B3 (CVB3) genome has been proposed to function as a cis-acting replication element (CRE). It is shown here that disruption of this structure indeed interfered with viral RNA replication in vivo and abolished uridylylation

  18. A minimal dose of electrically induced muscle activity regulates distinct gene signaling pathways in humans with spinal cord injury.

    Directory of Open Access Journals (Sweden)

    Michael A Petrie

    Full Text Available Paralysis after a spinal cord injury (SCI induces physiological adaptations that compromise the musculoskeletal and metabolic systems. Unlike non-SCI individuals, people with spinal cord injury experience minimal muscle activity which compromises optimal glucose utilization and metabolic control. Acute or chronic muscle activity, induced through electrical stimulation, may regulate key genes that enhance oxidative metabolism in paralyzed muscle. We investigated the short and long term effects of electrically induced exercise on mRNA expression of human paralyzed muscle. We developed an exercise dose that activated the muscle for only 0.6% of the day. The short term effects were assessed 3 hours after a single dose of exercise, while the long term effects were assessed after training 5 days per week for at least one year (adherence 81%. We found a single dose of exercise regulated 117 biological pathways as compared to 35 pathways after one year of training. A single dose of electrical stimulation increased the mRNA expression of transcriptional, translational, and enzyme regulators of metabolism important to shift muscle toward an oxidative phenotype (PGC-1α, NR4A3, IFRD1, ABRA, PDK4. However, chronic training increased the mRNA expression of specific metabolic pathway genes (BRP44, BRP44L, SDHB, ACADVL, mitochondrial fission and fusion genes (MFF, MFN1, MFN2, and slow muscle fiber genes (MYH6, MYH7, MYL3, MYL2. These findings support that a dose of electrical stimulation (∼10 minutes/day regulates metabolic gene signaling pathways in human paralyzed muscle. Regulating these pathways early after SCI may contribute to reducing diabetes in people with longstanding paralysis from SCI.

  19. Regulation of unbalanced electromagnetic moment in mutual loading systems of electric machines of traction rolling stock and multiple unit of mainline and industrial transport

    Directory of Open Access Journals (Sweden)

    A. M. Afanasov

    2014-12-01

    Full Text Available Purpose. The research data are aimed to identify the regulatory principles of unbalanced electromagnetic moment of mutually loaded electric machines of traction rolling stock and multiple unit of main and industrial transport. The purpose of this study is energy efficiency increase of the testing of traction electric machines of direct and pulse current using the improvement methods of their mutual loading, including the principles of automatic regulation of mutual loading system. Methodology. The general theoretical provisions and principles of system approach to the theoretical electric engineering, the theory of electric machines and theoretical mechanics are the methodological basis of this research. The known methods of analysis of electromagnetic and electromechanical processes in electrical machines of direct and pulse current are used in the study. Methods analysis of loading modes regulation of traction electric machines was conducted using the generalized scheme of mutual loading. It is universal for all known methods to cover the losses of idling using the electric power. Findings. The general management principles of mutual loading modes of the traction electric machines of direct and pulse current by regulating their unbalanced electric magnetic moment were developed. Regulatory options of unbalanced electromagnetic moment are examined by changing the difference of the magnetic fluxes of mutually loaded electric machines, the current difference of electric machines anchors, the difference of the angular velocities of electric machines shafts. Originality. It was obtained the scientific basis development to improve the energy efficiency test methods of traction electric machines of direct and pulse current. The management principles of mutual loading modes of traction electric machines were formulated. For the first time it is introduced the concept and developed the principles of regulation of unbalanced electromagnetic moment in

  20. From regulation through integration to? Three scenarios for the future of electricity in Europe

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, E.R. [Department of Management, University of Bologna, Bologna (Italy)

    1998-04-01

    A major change is underway in the European Union electricity sector, where the electricity market will be partly opened for competition. There is a required minimum opening of the generation market, although countries may exceed it. However, given the very different structures, technological mixes and national cultures, will a single market be possible? This paper outlines three different scenarios of how the European electricity market might evolve. (au)

  1. Unexpected severe consequences of Pikfyve deletion by aP2- or Aq-promoter-driven Cre expression for glucose homeostasis and mammary gland development.

    Science.gov (United States)

    Ikonomov, Ognian C; Sbrissa, Diego; Delvecchio, Khortnal; Rillema, James A; Shisheva, Assia

    2016-06-01

    Systemic deficiency of PIKfyve, the evolutionarily conserved phosphoinositide kinase synthesizing cellular PtdIns5P and PtdIns(3,5)P2 and implicated in insulin signaling, causes early embryonic death in mice. In contrast, mice with muscle-specific Pikfyve disruption have normal lifespan but exhibit early-age whole-body glucose intolerance and muscle insulin resistance, thus establishing the key role of muscle PIKfyve in glucose homeostasis. Fat and muscle tissues control postprandial glucose clearance through different mechanisms, raising questions as to whether adipose Pikfyve disruption will also trigger whole-body metabolic abnormalities, and if so, what the mechanism might be. To clarify these issues, here we have characterized two new mouse models with adipose tissue disruption of Pikfyve through Cre recombinase expression driven by adipose-specific aP2- or adiponectin (Aq) promoters. Whereas both mouse lines were ostensibly normal until adulthood, their glucose homeostasis and systemic insulin sensitivity were severely dysregulated. These abnormalities stemmed in part from accelerated fat-cell lipolysis and elevated serum FFA Intriguingly, aP2-Cre-PIKfyve(fl/fl) but not Aq-Cre-PIKfyve(fl/fl) females had severely impaired pregnancy-induced mammary gland differentiation and lactogenesis, consistent with aP2-Cre-mediated Pikfyve excision in nonadipogenic tissues underlying this defect. Intriguingly, whereas mammary glands from postpartum control and Aq-Cre-PIKfyve(fl/fl) mice or ex vivo mammary gland explants showed profound upregulation of PIKfyve protein levels subsequent to prolactin receptor activation, such increases were not apparent in aP2-Cre-PIKfyve(fl/fl) females. Collectively, our data identify for the first time that adipose tissue Pikfyve plays a key role in the mechanisms regulating glucose homeostasis and that the PIKfyve pathway is critical in mammary epithelial differentiation during pregnancy and lactogenesis downstream of prolactin receptor

  2. Heme oxygenase-1 expression protects the heart from acute injury caused by inducible Cre recombinase.

    Science.gov (United States)

    Hull, Travis D; Bolisetty, Subhashini; DeAlmeida, Angela C; Litovsky, Silvio H; Prabhu, Sumanth D; Agarwal, Anupam; George, James F

    2013-08-01

    The protective effect of heme oxygenase-1 (HO-1) expression in cardiovascular disease has been previously demonstrated using transgenic animal models in which HO-1 is constitutively overexpressed in the heart. However, the temporal requirements for protection by HO-1 induction relative to injury have not been investigated, but are essential to employ HO-1 as a therapeutic strategy in human cardiovascular disease states. Therefore, we generated mice with cardiac-specific, tamoxifen (TAM)-inducible overexpression of a human HO-1 (hHO-1) transgene (myosin heavy chain (MHC)-HO-1 mice) by breeding mice with cardiac-specific expression of a TAM-inducible Cre recombinase (MHC-Cre mice), with mice containing an hHO-1 transgene preceded by a floxed-stop signal. MHC-HO-1 mice overexpress HO-1 mRNA and the enzymatically active protein following TAM administration (40 mg/kg body weight on 2 consecutive days). In MHC-Cre controls, TAM administration leads to severe, acute cardiac toxicity, cardiomyocyte necrosis, and 80% mortality by day 3. This cardiac toxicity is accompanied by a significant increase in inflammatory cells in the heart that are predominantly neutrophils. In MHC-HO-1 mice, HO-1 overexpression ameliorates the depression of cardiac function and high mortality rate observed in MHC-Cre mice following TAM administration and attenuates cardiomyocyte necrosis and neutrophil infiltration. These results highlight that HO-1 induction is sufficient to prevent the depression of cardiac function observed in mice with TAM-inducible Cre recombinase expression by protecting the heart from necrosis and neutrophil infiltration. These findings are important because MHC-Cre mice are widely used in cardiovascular research despite the limitations imposed by Cre-induced cardiac toxicity, and also because inflammation is an important pathological component of many human cardiovascular diseases.

  3. Conditional Wwox deletion in mouse mammary gland by means of two Cre recombinase approaches.

    Directory of Open Access Journals (Sweden)

    Brent W Ferguson

    Full Text Available Loss of WWOX expression has been reported in many different cancers including breast cancer. Elucidating the function of this gene in adult tissues has not been possible with full Wwox knockout models. Here we characterize the first conditional models of Wwox ablation in mouse mammary epithelium utilizing two transgenic lines expressing Cre recombinase, keratin 5-Cre (BK5-Cre and MMTV-Cre. In the BK5-Cre model we observed very efficient Wwox ablation in KO mammary glands. However, BK5-Cre Wwox KO animals die prematurely for unknown reasons. In the MMTV-Cre model we observed significant ablation of Wwox in mammary epithelium with no effect on survival. In both of these models we found that Wwox deletion resulted in impaired mammary branching morphogenesis. We demonstrate that loss of Wwox is not carcinogenic in our KO models. Furthermore, no evidence of increase proliferation or development of premalignant lesions was observed. In none of the models did loss of a single Wwox allele (i.e. haploinsufficiency have any observable phenotypic effect in mammary gland. To better understand the function of Wwox in the mammary gland, transcriptome profiling was performed. We observed that Wwox ablation results in the deregulation of genes involved in various cellular processes. We found that expression of the non-canonical Wnt ligand, Wnt5a, was significantly upregulated in Wwox KO mammary epithelium. Interestingly, we also determined that components of the Jak/Stat3 signaling pathway were upregulated in KO mice and this correlated with a very robust increase in phospho-Stat3 signaling, which warrants further testing. Even though the loss of Wwox expression in breast and other cancers is very well documented, our findings suggest that Wwox does not act as a classical tumor suppressor as previously thought.

  4. Cre Fused with RVG Peptide Mediates Targeted Genome Editing in Mouse Brain Cells In Vivo.

    Science.gov (United States)

    Zou, Zhiyuan; Sun, Zhaolin; Li, Pan; Feng, Tao; Wu, Sen

    2016-12-14

    Cell penetrating peptides (CPPs) are short peptides that can pass through cell membranes. CPPs can facilitate the cellular entry of proteins, macromolecules, nanoparticles and drugs. RVG peptide (RVG hereinafter) is a 29-amino-acid CPP derived from a rabies virus glycoprotein that can cross the blood-brain barrier (BBB) and enter brain cells. However, whether RVG can be used for genome editing in the brain has not been reported. In this work, we combined RVG with Cre recombinase for bacterial expression. The purified RVG-Cre protein cut plasmids in vitro and traversed cell membranes in cultured Neuro2a cells. By tail vein-injecting RVG-Cre into Cre reporter mouse lines mTmG and Rosa26lacZ, we demonstrated that RVG-Cre could target brain cells and achieve targeted somatic genome editing in adult mice. This direct delivery of the gene-editing enzyme protein into mouse brains with RVG is much safer than plasmid- or viral-based methods, holding promise for further applications in the treatment of various brain diseases.

  5. Cell-Specific Cre Strains For Genetic Manipulation in Salivary Glands.

    Directory of Open Access Journals (Sweden)

    Eri O Maruyama

    Full Text Available The secretory acinar cells of the salivary gland are essential for saliva secretion, but are also the cell type preferentially lost following radiation treatment for head and neck cancer. The source of replacement acinar cells is currently a matter of debate. There is evidence for the presence of adult stem cells located within specific ductal regions of the salivary glands, but our laboratory recently demonstrated that differentiated acinar cells are maintained without significant stem cell contribution. To enable further investigation of salivary gland cell lineages and their origins, we generated three cell-specific Cre driver mouse strains. For genetic manipulation in acinar cells, an inducible Cre recombinase (Cre-ER was targeted to the prolactin-induced protein (Pip gene locus. Targeting of the Dcpp1 gene, encoding demilune cell and parotid protein, labels intercalated duct cells, a putative site of salivary gland stem cells, and serous demilune cells of the sublingual gland. Duct cell-specific Cre expression was attempted by targeting the inducible Cre to the Tcfcp2l1 gene locus. Using the R26Tomato Red reporter mouse, we demonstrate that these strains direct inducible, cell-specific expression. Genetic tracing of acinar cells using PipGCE supports the recent finding that differentiated acinar cells clonally expand. Moreover, tracing of intercalated duct cells expressing DcppGCE confirms evidence of duct cell proliferation, but further analysis is required to establish that renewal of secretory acinar cells is dependent on stem cells within these ducts.

  6. Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.

    Science.gov (United States)

    Calaminus, Simon D J; Guitart, Amelie V; Guitart, Amelie; Sinclair, Amy; Schachtner, Hannah; Watson, Steve P; Holyoake, Tessa L; Kranc, Kamil R; Machesky, Laura M

    2012-01-01

    The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage.

  7. Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.

    Directory of Open Access Journals (Sweden)

    Simon D J Calaminus

    Full Text Available The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4 promoter (Pf4-Cre, was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs, including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15-60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage.

  8. Nationwide Surveillance of Clinical Carbapenem-resistant Enterobacteriaceae (CRE Strains in China

    Directory of Open Access Journals (Sweden)

    Rong Zhang

    2017-05-01

    Full Text Available The increasing incidence of carbapenem-resistant Enterobacteriaceae (CRE - mediated hospital infections in China prompted a need to investigate the genetic basis of emergence of such strains. A nationwide survey was conducted in China covering a total of 1105 CRE strains collected from 25 geographical locales with results showing that acquisition of two carbapenemase genes, blaKPC-2 and blaNDM, was responsible for phenotypic resistance in 90% of the CRE strains tested (58% and 32% respectively, among which several major strain types, such as ST11 of K. pneumoniae and ST131/ST167 of E. coli, were identified, suggesting that dissemination of specific resistant clones is mainly responsible for emergence of new CRE strains. Prevalence of the fosA3 gene which mediates fosfomycin resistance, was high, while the colistin resistance determinant mcr-1 was rarely present in these isolates. Consistently, the majority of the blaNDM-bearing plasmids recoverable from the test strains belonged to IncX3, which contained a common core structure, blaNDM-blaMBL-trpF. Likewise, the core structure of ISKpn27-blaKPC-2-ISKpn2 was observed among plasmids harboring the blaKPC-2 gene, although they were genetically more divergent. In conclusion, the increasing prevalence of CRE strains in China is attributed to dissemination of conservative mobile elements carrying blaNDM or blaKPC-2 on conjugative and non-conjugative plasmids.

  9. Molecular switch of Cre/loxP for radiation modulated gene therapy on hepatoma

    Science.gov (United States)

    Hsieh, Ya-Ju; Chen, Fu-Du; Wang, Fu Hui; Ke, Chien Chih; Wang, Hsin-Ell; Liu, Ren-Shyan

    2007-02-01

    For the purpose of enhancement of AFP promoter for the use of radiation modulated gene therapy for hepatocellular carcinoma (HCC), we combined hepatitis B virus (HBV) enhancer II with AFP promoter which shows the selectivity to the target cells to control the Cre/loxP system. Different gene constructs, pE4luc, pE4Tk, EIIAPA-Cre, E4CMV-STOP-Tk and chimeric promoters combined with HBV enhancer were constructed and transfected into HepG2, HeLa and NIH-3T3 cell lines. Cell experiments revealed that E4 enhancer responses to radiation best after 60 h irradiation at a dose range of 5-7 Gy in HepG2 stable clone. The EIIAPA promoter provided high specificity to hepatoma and activated the Cre downstream and removed the stop cassette only in hepatoma cells. After removal of the stop cassette, the E4 response to radiation could encode more Tk protein and kill more tumor cells. In summary, the chimeric EIIAPA promoter can stringently control the expression of Cre recombinase only in HCC. The radiation effect of the EIIAPA-Cre and E4CMV-STOP-Tk system shows promising results in terms of cell survival of HCC.

  10. Negative Effects of Antimonopoly Regulation on the Russian Electric Power Industry

    National Research Council Canada - National Science Library

    Elena NEPRINTSEVA; Stanislav SHUBIN

    2017-01-01

    With the antimonopoly regulation in the domestic economy getting more stringent an analysis of the current measures of antimonopoly regulation in terms of their efficiency is now becoming ever more relevant...

  11. Stochastic Optimal Regulation Service Strategy for a Wind Farm Participating in the Electricity Market

    DEFF Research Database (Denmark)

    Zhang, Baohua; Hu, Weihao; Chen, Zhe

    2015-01-01

    As modern wind farmshave the ability to provideregulation service for the power system, wind power plant operators may be motivated to participate in the regulating market to maximize their profit.In this paper, anoptimal regulation servicestrategy for a wind farm to participate in the regulating...

  12. Measurement of Heat Losses on The Milking Machine Electric Motor at Various Regulations of Vacuum Using Methods of Thermal Imagery

    Directory of Open Access Journals (Sweden)

    Jan Kudělka

    2014-01-01

    Full Text Available To ensure the desirable vacuum in the milking machines, use is currently made predominantly of rotary vacuum pumps. These vacuum pumps are driven by a squirrel-cage induction motor. Until recently, the vacuum in the system to achieve the required value was controlled by a main control valve sucking in ambient air into the system. During the milking process itself and during other activities (flushing, sanitation, this control method consumed a large amount of electricity. The technical solution to electricity demand reduction was introduced with the emergence and development of frequency converters. The frequency converters control the operation of the asynchronous electric motor so that the actual delivery of the vacuum pumps equals the volume of air sucked into the vacuum pipe. The motor supply by the frequency converter brings about a host of adverse phenomena. This paper is dedicated to motor heating and heat losses on the surface of the electric motor at different regulations of vacuum in milking machines. The objective of the paper is to determine the immediate specific heat flows along the surface of the electric motor of the milking machine during milking using a control valve regulation and a control using the frequency converter, and compare the resulting value. The specific heat flows were determined by means of a non-traditional method of temperature field measurement using a system of thermal imagery. The calculated and measured data obtained from both these systems were statistically evaluated and compared. Use was made of a milking machine located in the cooperative Hospodářské obchodní družstvo (HOD Jabloňov.

  13. BIOCHEMICAL AND PHYLOGENETIC STUDIES OF CreD OF Corynebacterium glutamicum

    Directory of Open Access Journals (Sweden)

    Muhammad Tausif Chaudhry

    2015-06-01

    Full Text Available CreD characterized as Mg2+-dependent phosphohydrolase with conserved HD domain was involved in 4-cresol metabolism in Corynebacterium glutamicum. Native molecular mass of 54 kDa suggested that the biological unit is a dimer. No deoxynucleotide triphosphate triphosphohydrolase (dNTPase activity was detected for CreD. The apparent Km and Vmax values for 4-nitrophenyl phosphate were 0.35 mM and 16.23 M min-1 mg-1, respectively, while calculated values for kcat and kcat/Km were 0.4 s-1 and 1.14103 M-1 s-1, respectively. Among thiol group inhibitors, iodoacetic acid significantly inhibited phosphohydrolase activity. Sequence identity and phylogenetic analysis suggested universal existence of CreD homologues. Involvement of HD-domain hydrolase in aromatic degradation has not been reported before.

  14. Efficient B cell depletion via diphtheria toxin in CD19-Cre/iDTR mice.

    Directory of Open Access Journals (Sweden)

    Filiz Demircik

    Full Text Available B cells were first discovered as antibody producing cells, as B-1 B cells and finally as effector cells. In recent years their capacity to serve as antigen presenting cells is increasingly appreciated, and better tools are needed to study their function. We have previously described a new mouse model, the iDTR mice, that allow for the Cre-mediated expression of the diphtheria toxin receptor, thus rendering cells that express the Cre-recombinase sensitivity to diphtheria toxin. Herein we describe a new mouse line, the B-DTR mice, where the CD19-Cre was crossed to the iDTR mice. B-DTR allows for the efficient and cost-effective depletion of different B cell subpopulations, but only partially plasma cells. These mice can therefore be used to study the importance of B cells versus plasma cells in different immune responses and autoimmune diseases.

  15. PREPARATION OF PLANT TRANSFORMATION VECTOR CONTAINING “SELF-EXCISION” CRE/LOXP SYSTEM

    Directory of Open Access Journals (Sweden)

    Jana Moravčíková

    2012-02-01

    Full Text Available This work is focused on preparation of the plant transformation vector pZP6 containing “self-excision” Cre/loxP system. The T-DNA of binary vector consists of the cre recombinase gene driven by the Arabidopsis DLL promoter and the nptII expression unit flanked by two loxP sites in direct orientation. The gus reporter gene controlled by the double CaMV 35S promoter was placed out of the loxP embedded DNA. To confirm functionality of the Cre/loxP system, the pZP6 was analyzed for correct removal of the loxP embedded sequence in E. coli. The pZP6 was transformed into two bacterial strains A. tumefaciens AGLO and LBA 4404. Its stability in agrobacteria was evaluated by restriction analyses.

  16. Short-Term Synaptic Plasticity Regulation in Solution-Gated Indium-Gallium-Zinc-Oxide Electric-Double-Layer Transistors.

    Science.gov (United States)

    Wan, Chang Jin; Liu, Yang Hui; Zhu, Li Qiang; Feng, Ping; Shi, Yi; Wan, Qing

    2016-04-20

    In the biological nervous system, synaptic plasticity regulation is based on the modulation of ionic fluxes, and such regulation was regarded as the fundamental mechanism underlying memory and learning. Inspired by such biological strategies, indium-gallium-zinc-oxide (IGZO) electric-double-layer (EDL) transistors gated by aqueous solutions were proposed for synaptic behavior emulations. Short-term synaptic plasticity, such as paired-pulse facilitation, high-pass filtering, and orientation tuning, was experimentally emulated in these EDL transistors. Most importantly, we found that such short-term synaptic plasticity can be effectively regulated by alcohol (ethyl alcohol) and salt (potassium chloride) additives. Our results suggest that solution gated oxide-based EDL transistors could act as the platforms for short-term synaptic plasticity emulation.

  17. Regulating the Electricity Supply Industry in Nigeria. An Assessment of Consumer's Attitudes

    Directory of Open Access Journals (Sweden)

    Olawale Ibrahim Olateju

    2009-05-01

    Full Text Available The power sector is critical to the development of any country and Nigeria is no exception, incessant power outrage has been the bane of economic development in the country. For the past three decades, the power sector of the country has been plagued by a lot of problems ranging from low power generation and distribution, decaying facilities most of which were commissioned before and shortly after the country’s independence. The interaction of the electric power industry with climate is manifested both in the effect that severe weather has on the power system and through the contribution of electric power to the production of greenhouse gas (GHG and other pollutants.The paper examines and provides answers to (i the nature of consumers’ attitudes towards power consumption and (ii suggest ways of informing the consumers on the need to conserve power when not in use. This study was carried out in Alimosho Local Government Area of Lagos State, south-west Nigeria. The findings show that 61 percent of electricity consumers’ agreed that rationing of electricity consumption would stabilized the electricity distribution, whereas 39 percent were not in agreement. The study also shows that about 90 percent of the consumers were not satisfied with the services being offered by the Government owned company, while the remaining 10 percent were satisfied. We suggest the authority take to prepaid method of electricity consumption and also intensify efforts to enlighten the public on the need to conserve power.

  18. Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants.

    Science.gov (United States)

    Zhang, W; Subbarao, S; Addae, P; Shen, A; Armstrong, C; Peschke, V; Gilbertson, L

    2003-11-01

    After the initial transformation and tissue culture process is complete, selectable marker genes, which are used in virtually all transformation approaches, are not required for the expression of the gene of interest in the transgenic plants. There are several advantages to removing the selectable marker gene after it is no longer needed, such as enabling the reuse of selectable markers and simplifying transgene arrays. We have tested the Cre/ lox system from bacteriophage P1 for its ability to precisely excise stably integrated marker genes from chromosomes in transgenic maize plants. Two strategies, crossing and autoexcision, have been tested and demonstrated. In the crossing strategy, plants expressing the Cre recombinase are crossed with plants bearing a transgene construct in which the selectable marker gene is flanked by directly repeated lox sites. Unlike previous reports in which incomplete somatic and germline excision were common, in our experiments complete somatic and germline marker gene excision occurred in the F(1) plants from most crosses with multiple independent Cre and lox lines. In the autoexcision strategy, the cre gene, under the control of a heat shock-inducible promoter, is excised along with the nptII marker gene. Our results show that a transient heat shock treatment of primary transgenic callus is sufficient for inducing cre and excising the cre and nptII genes. Genetic segregation and molecular analysis confirmed that marker gene removal is precise, complete and stable. The autoexcision strategy provides a way of removing the selectable marker gene from callus or other tissues such as embryos and kernels.

  19. A pink mouse reports the switch from red to green fluorescence upon Cre-mediated recombination

    Directory of Open Access Journals (Sweden)

    Hartwich Heiner

    2012-06-01

    Full Text Available Abstract Background Targeted genetic modification in the mouse becomes increasingly important in biomedical and basic science. This goal is most often achieved by use of the Cre/loxP system and numerous Cre-driver mouse lines are currently generated. Their initial characterization requires reporter mouse lines to study the in vivo spatiotemporal activity of Cre. Findings Here, we report a dual fluorescence reporter mouse line, which switches expression from the red fluorescent protein mCherry to eGFP after Cre-mediated recombination. Both fluorescent proteins are expressed from the ubiquitously active and strong CAGGS promoter. Among the founders, we noticed a pink mouse line, expressing high levels of the red fluorescent protein mCherry throughout the entire body. Presence of mCherry in the living animal as well as in almost all organs was clearly visible without optical equipment. Upon Cre-activity, mCherry expression was switched to eGFP, demonstrating functionality of this reporter mouse line. Conclusions The pink mouse presented here is an attractive novel reporter line for fluorescence-based monitoring of Cre-activity. The high expression of mCherry, which is visible to the naked eye, facilitates breeding and crossing, as no genotyping is required to identify mice carrying the reporter allele. The presence of two fluorescent proteins allows in vivo monitoring of recombined and non-recombined cells. Finally, the pink mouse is an eye-catching animal model to demonstrate the power of transgenic techniques in teaching courses.

  20. Highly Efficient Sex Chromosome Interchanges Produced By I-CreI Expression in Drosophila

    OpenAIRE

    Maggert, Keith A; Golic, Kent G

    2005-01-01

    The homing endonuclease I-CreI recognizes a site in the gene encoding the 23S rRNA of Chlamydomonas reinhardtii. A very similar sequence is present in the 28S rRNA genes that are located on the X and Y chromosomes of Drosophila melanogaster. In this work we show that I-CreI expression in Drosophila is capable of causing induced DNA damage and eliciting cell cycle arrest. Expression also caused recombination between the X and Y chromosomes in the heterochromatic regions where the rDNA is locat...

  1. Concurrent Provision of Frequency Regulation and Overvoltage Support by Electric Vehicles in a Real Danish Low Voltage Network

    DEFF Research Database (Denmark)

    Knezovic, Katarina; Marinelli, Mattia; Andersen, Peter Bach

    2014-01-01

    Expected deployment of electric vehicles (EVs) introduces big technical challenges for power system operation, but also offers advantages provided that EVs are not considered merely as passive loads. With the development of Vehicle-to-Grid technology, EVs will be able to provide a number of ancil......Expected deployment of electric vehicles (EVs) introduces big technical challenges for power system operation, but also offers advantages provided that EVs are not considered merely as passive loads. With the development of Vehicle-to-Grid technology, EVs will be able to provide a number...... of ancillary services for grid support, e.g. implemented electronic equipment will allow them to exchange reactive power with the grid for voltage regulation while using active power for other services. This paper investigates the concurrent provision of local and system wide services from EVs in a real Danish...

  2. Managing the transition from regulated electric utility to energy marketing company

    Energy Technology Data Exchange (ETDEWEB)

    Krapels, E.N.

    1977-01-01

    Companies that hope to succeed in converging energy service business must plan to trade bundled and unbundled energy commodities in paper as well as physical markets. The winners will be those who realize the immensity and crucial importance of the former. Many traditional electric utilities are spending most of their time defensively on stranded asset issues, and only token amounts of time proactively on how to become a Btu company. The vacuum they are creating is being filled by those companies - Enron, UtiliCorp United, Duke Power, and a few others who have made preparing for the future their core mission rather than a sideshow. Important as the stranded asset issue is - and it is especially critical in nuclear-heavy regions like New England - the author believes that senior executives of all electric utilities must immediately relegate this {open_quotes}work of the past{close_quotes} to second place. It is urgent that they now begin to do what Enron decided to do almost ten years ago: acknowledge that an enormous paradigm shift has taken place in the energy industry and devote all available brainpower to preparing for that change. Electric utilities are on the threshold of an era in which electricity prices will be determined, not only by supply and demand as traditionally understood, but by the supply and demand for electricity in paper markets. In traditional market nomenclature, prices are determined by supply and demand. In paper markets, while the analytical metaphor is identical, the language and dynamics are different: Prices will emerge from the interplay of those who are {open_quotes}short{close_quotes} (i.e., willing to sell in the power futures markets at given prices). Electric utilities must master the dynamics of both markets. No less than their corporate survival is at stake.

  3. Large-scale integration of off-shore wind power and regulation strategies of cogeneration plants in the Danish electricity system

    DEFF Research Database (Denmark)

    Østergaard, Poul Alberg

    2005-01-01

    The article analyses how the amount of a small-scale CHP plants and heat pumps and the regulation strategies of these affect the quantity of off-shore wind power that may be integrated into Danish electricity supply......The article analyses how the amount of a small-scale CHP plants and heat pumps and the regulation strategies of these affect the quantity of off-shore wind power that may be integrated into Danish electricity supply...

  4. Electric Substations

    Data.gov (United States)

    Department of Homeland Security — Substations. Substations are facilities and equipment that switch, transform, or regulate electric voltage. The Substations feature class includes taps, a location...

  5. The rule of law approach to regulating electricity supply in Nigeria ...

    African Journals Online (AJOL)

    With the transfer of government shares in 17 electricity generation and distribution companies in November 2013, Nigeria crossed the Rubicon in the privatization process. This process began in earnest in 2003 with the liberalization of the telecom sector as part of a strategic reform to improve efficiency in these utility sectors ...

  6. Radical prosumer innovations in the electricity sector and the impact on prosumer regulation

    NARCIS (Netherlands)

    Lavrijssen, Saskia; Carrillo, Arturo

    2017-01-01

    The electricity sector is in a transition towards a Smart Energy System where the roles of private and institutional actors are evolving. This work deals with the influence of some technological innovations, enabling social innovations such as peer to peer trading and the participation in local

  7. Provision of regulating - and reserve power by electric vehicle owners in the Dutch market

    NARCIS (Netherlands)

    Hoogvliet, Tim; Litjens, G.B.M.A.|info:eu-repo/dai/nl/41129766X; van Sark, W.G.J.H.M.|info:eu-repo/dai/nl/074628526

    2017-01-01

    Recent years have shown a large increase in electric vehicles (EVs), which could make a significant contribution meeting European, national and municipal energy- and climate goals. However, most EVs are not used for about 90% of the time, which makes their batteries available for other purposes. One

  8. Natural monopolies regulation: the electric power transmission tariffs case; Regulacao dos monopolios naturais: o caso das tarifas de transmissao

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Ricardo Henrique dos; Santos, Rosana Rodrigues dos; Sauer, Ildo [Sao Paulo Univ., SP (Brazil). Programa Interunidades de Pos-Graduacao em Energia]. E-mail: rosana2@uol.com.br

    1999-07-01

    The central issue of debate was the need to align the energy sector's options and organization with changing global patterns of economic and social development, characterized by the increasing role played by the private sector, greater integration in the world economy, and new economic and social priorities such as efficiency, decentralization, deregulation, and a closer attention to environmental issues. In this context, the aim of this work is to review the natural monopolies theory, regulation regimes, and the energy transmission tariffs of Brazilian electric power sector.

  9. Transgenic Mice Expressing a Ligand-Inducible Cre Recombinase in Osteoblasts and Odontoblasts

    Science.gov (United States)

    Kim, Jung-Eun; Nakashima, Kazuhisa; de Crombrugghe, Benoit

    2004-01-01

    The skeleton supports body structures in vertebrates and helps maintain calcium homeostasis throughout life. Disruption of genes involved in mammalian bone formation has often led to embryonic lethality, hence preventing study of these genes’ role in adult animals. To develop a usable tool for such study, we generated transgenic mice in which a 2.3-kb mouse Col1a1 proximal promoter, which is active in all osteoblasts, drives a transgene coding for a polypeptide consisting of Cre recombinase fused to a mutated ligand-binding domain of the estrogen receptor. In this Col1a1-CreERT2 mouse line, expression patterns of the transgene and of the resulting Cre-mediated DNA recombination are analyzed by crossing with ROSA26 reporter mice and by measurement of β-galactosidase activity and X-gal staining. Exposure to 4-hydroxytamoxifen induced Cre-mediated recombination in osteoblasts in virtually all bones and in odontoblasts in teeth of both embryos and postnatal mice. The generation of these transgenic mice provides a new and important tool with which to study the function of specific genes in bone and tooth physiology and diseases in intact animals after birth. PMID:15579432

  10. STIM1fl/fl Ksp-Cre Mouse has Impaired Renal Water Balance

    Directory of Open Access Journals (Sweden)

    Liudmila Cebotaru

    2016-06-01

    Full Text Available Background/AIM: STIM1 is as an essential component in store operated Ca2+ entry. However give the paucity of information on the role of STIM1 in kidney, the aim was to study the function of STIM1 in the medulla of the kidney. Methods: we crossed a Ksp-cre mouse with another mouse containing two loxP sites flanking Exon 6 of STIM1. The Ksp-cre mouse is based upon the Ksp-cadherin gene promoter which expresses cre recombinase in developing nephrons, collecting ducts (SD and thick ascending limbs (TAL of the loop of Henle. Results: The offspring of these mice are viable without gross morphological changes, however, we noticed that the STIM1 Ksp-cre knockout mice produced more urine compared to control. To examine this more carefully, we fed mice low (LP and high protein (HP diets respectively. When mice were fed HP diet STIM1 ko mice had significantly increased urinary volume and lower specific gravity compared to wt mice. In STIM1 ko mice fed HP diet urine creatinine and urea were significantly lower compared to wt mice fed HP diet, however the fractional excretion was the same. Conclusion: These data support the idea that STIM1 ko mice have impaired urinary concentrating ability when challenged with HP diet is most likely caused by impaired Ca2+-dependent signal transduction through the vasopressin receptor cascade.

  11. Robust cre-mediated recombination in small intestinal stem cells utilizing the olfm4 locus

    NARCIS (Netherlands)

    Schuijers, Jurian; van der Flier, Laurens G; van Es, Johan; Clevers, Hans

    2014-01-01

    The epithelium of the small intestine is the most rapidly self-renewing tissue in mammals. We previously demonstrated the existence of a long-lived pool of cycling stem cells defined by Lgr5 expression at the bottom of intestinal crypts. An Lgr5-eGFP-IRES-CreERT2 knockin allele has been instrumental

  12. The FEM simulation of continuous rotary extrusion (CRE) of aluminum alloy AA3003

    Science.gov (United States)

    Rajendran, Nijenthan; Valberg, Henry; Misiolek, Wojciech Z.

    2017-10-01

    Continuous Rotary Extrusion (CRE) process is also known in literature under Conform TM name and it is mainly used for the continuous extrusion of Aluminum and Copper alloys. CRE use a feedstock in the form of rod, powders and chips, which are fed into the groove of the rotating wheel. As the wheel rotates the feedstock moves along with it due to friction with the wheel. Once the feedstock reaches the abutment the material deforms plastically and it is extruded through the die. CRE has lot to offer when compared to other more conventional extrusion processes such as low energy input, no limit in billet length as it is a continuous process as well as improved material physical properties due to plastic deformation under constant parameters. In this work a FEM model has been developed using Deform TM 3D, to study the metal flow and state variables of AA3003 CRE extrusion. The effect of extrusion wheel velocity has been investigated. The results show that increase in wheel velocity will heat up the feedstock metal due to high shear deformation and higher friction, which significantly changes metal flow conditions at the die exit.

  13. Sox9- and Scleraxis-Cre Lineage Fate Mapping in Aortic and Mitral Valve Structures

    Directory of Open Access Journals (Sweden)

    Blair F. Austin

    2014-09-01

    Full Text Available Heart valves are complex structures composed of a heterogeneous population of valve interstitial cells (VICs, an overlying endothelium and highly organized layers of extracellular matrix. Alterations in valve homeostasis are characteristic of dysfunction and disease, however the mechanisms that initiate and promote valve pathology are poorly understood. Advancements have been largely hindered by the limited availability of tools for gene targeting in heart valve structures during embryogenesis and after birth. We have previously shown that the transcription factors Sox9 and Scleraxis (Scx are required for heart valve formation and in this study we describe the recombination patterns of Sox9- and Scx-Cre lines at differential time points in aortic and mitral valve structures. In ScxCre; ROSA26GFP mice, recombination is undetected in valve endothelial cells (VECs and low in VICs during embryogenesis. However, recombination increases in VICs from post natal stages and by 4 weeks side-specific patterns are observed. Using the inducible Sox9CreERT2 system, we observe recombination in VECs and VICs in the embryo, and high levels are maintained through post natal and juvenile stages. These Cre-drivers provide the field with new tools for gene targeting in valve cell lineages during differential stages of embryonic and post natal maturation and maintenance.

  14. High-fidelity Glucagon-CreER mouse line generated by CRISPR-Cas9 assisted gene targeting

    Directory of Open Access Journals (Sweden)

    Amanda M. Ackermann

    2017-03-01

    Conclusions: We successfully derived a Gcg-CreERT2 mouse line that expresses CreERT2 in pancreatic α-cells and enteroendocrine L-cells without disrupting preproglucagon gene expression. These mice will be a useful tool for performing temporally controlled genetic manipulation specifically in these cell types.

  15. NF1 Is an Effector and Regulator of the GPCR Signaling in the Nervous System

    Science.gov (United States)

    2015-04-01

    of NF1 by infecting differentiated cultures with AAV carrying Cre recombinase (Task 2 A and B). We used these cultures along with uninfected...activation and regulation of its signaling to downstream protein kinases (Task 3). We infected neurons with the AAV virus encoding Cre-recombinase...cultured striatal neurons on MAPK signaling. Cultured neurons from NF1flx/flx mice were infected with either AAV -Cre deleting NF1 or control AAV

  16. Altered Baseline and Nicotine-Mediated Behavioral and Cholinergic Profiles in ChAT-Cre Mouse Lines.

    Science.gov (United States)

    Chen, Edison; Lallai, Valeria; Sherafat, Yasmine; Grimes, Nickolas P; Pushkin, Anna N; Fowler, J P; Fowler, Christie D

    2018-01-25

    The recent development of transgenic rodent lines expressing cre recombinase in a cell-specific manner, along with advances in engineered viral vectors, has permitted in-depth investigations into circuit function. However, emerging evidence has begun to suggest that genetic modifications may introduce unexpected caveats. In the current studies, we sought to extensively characterize male and female mice from both the ChAT (BAC) -Cre mouse line, created with the bacterial artificial chromosome (BAC) method, and ChAT (IRES) -Cre mouse line, generated with the internal ribosome entry site (IRES) method. ChAT (BAC) -Cre transgenic and wildtype mice did not differ in general locomotor behavior, anxiety measures, drug-induced cataplexy, nicotine-mediated hypolocomotion, or operant food training. However, ChAT (BAC) -Cre transgenic mice did exhibit significant deficits in intravenous nicotine self-administration, which paralleled an increase in vesicular acetylcholine transporter (VAChT) and choline acetyltransferase (ChAT) hippocampal expression. For the ChAT (IRES) -Cre line, transgenic mice exhibited deficits in baseline locomotor, nicotine-mediated hypolocomotion, and operant food training compared to wildtype and hemizygous littermates. No differences among ChAT (IRES) -Cre wildtype, hemizygous and transgenic littermates were found in anxiety measures, drug-induced cataplexy, and nicotine self-administration. Given that increased cre expression was present in the ChAT (IRES) -Cre transgenic mice, as well as a decrease in ChAT expression in the hippocampus, altered neuronal function may underlie behavioral phenotypes. In contrast, ChAT (IRES) -Cre hemizygous mice were more similar to wildtype mice in both protein expression and the majority of behavioral assessments. As such, interpretation of data derived from ChAT-Cre rodents must consider potential limitations dependent on the line and/or genotype employed in research investigations. SIGNIFICANCE STATEMENT Altered

  17. Electric drive vehicle systems : suggested changes to large truck and motorcoach regulations and inspection procedures.

    Science.gov (United States)

    2015-09-01

    This report provides recommendations for changes to the Federal Motor Carrier Safety Regulations (FMCSRs), : North American Standard (NAS) inspection procedures, and Out-of-Service (OOS) criteria that are needed to : accommodate and facilitate the us...

  18. Overview of BioCreAtIvE: critical assessment of information extraction for biology

    Directory of Open Access Journals (Sweden)

    Hirschman Lynette

    2005-05-01

    Full Text Available Abstract Background The goal of the first BioCreAtIvE challenge (Critical Assessment of Information Extraction in Biology was to provide a set of common evaluation tasks to assess the state of the art for text mining applied to biological problems. The results were presented in a workshop held in Granada, Spain March 28–31, 2004. The articles collected in this BMC Bioinformatics supplement entitled "A critical assessment of text mining methods in molecular biology" describe the BioCreAtIvE tasks, systems, results and their independent evaluation. Results BioCreAtIvE focused on two tasks. The first dealt with extraction of gene or protein names from text, and their mapping into standardized gene identifiers for three model organism databases (fly, mouse, yeast. The second task addressed issues of functional annotation, requiring systems to identify specific text passages that supported Gene Ontology annotations for specific proteins, given full text articles. Conclusion The first BioCreAtIvE assessment achieved a high level of international participation (27 groups from 10 countries. The assessment provided state-of-the-art performance results for a basic task (gene name finding and normalization, where the best systems achieved a balanced 80% precision / recall or better, which potentially makes them suitable for real applications in biology. The results for the advanced task (functional annotation from free text were significantly lower, demonstrating the current limitations of text-mining approaches where knowledge extrapolation and interpretation are required. In addition, an important contribution of BioCreAtIvE has been the creation and release of training and test data sets for both tasks. There are 22 articles in this special issue, including six that provide analyses of results or data quality for the data sets, including a novel inter-annotator consistency assessment for the test set used in task 2.

  19. Transgenic mice for a tamoxifen-induced, conditional expression of the Cre recombinase in osteoclasts.

    Directory of Open Access Journals (Sweden)

    Maria Arantzazu Sanchez-Fernandez

    Full Text Available BACKGROUND: Studies on osteoclasts, the bone resorbing cells, have remained limited due to the lack of transgenic mice allowing the conditional knockout of genes in osteoclasts at any time during development or adulthood. METHODOLOGY/PRINCIPAL FINDING: We report here on the generation of transgenic mice which specifically express a tamoxifen-inducible Cre recombinase in osteoclasts. These mice, generated on C57BL/6 and FVB background, express a fusion Cre recombinase-ERT2 protein whose expression is driven by the promoter of cathepsin K (CtsK, a gene highly expressed in osteoclasts. We tested the cellular specificity of Cre activity in CtsKCreERT2 strains by breeding with Rosa26LacZ reporter mice. PCR and histological analyses of the CtsKCreERT2LacZ positive adult mice and E17.5 embryos show that Cre activity is restricted largely to bone tissue. In vitro, primary osteoclasts derived from the bone marrow of CtsKCreERT2+/-LacZ+/- adult mice show a Cre-dependent β-galactosidase activity after tamoxifen stimulation. CONCLUSIONS/SIGNIFICANCE: We have generated transgenic lines that enable the tamoxifen-induced, conditional deletion of loxP-flanked genes in osteoclasts, thus circumventing embryonic and postnatal gene lethality and avoiding gene deletion in other cell types. Such CtsKCreERT2 mice provide a convenient tool to study in vivo the different facets of osteoclast function in bone physiology during different developmental stages and adulthood of mice.

  20. Real option valuation of a decremental regulation service provided by electricity storage.

    Science.gov (United States)

    Szabó, Dávid Zoltán; Martyr, Randall

    2017-08-13

    This paper is a quantitative study of a reserve contract for real-time balancing of a power system. Under this contract, the owner of a storage device, such as a battery, helps smooth fluctuations in electricity demand and supply by using the device to increase electricity consumption. The battery owner must be able to provide immediate physical cover, and should therefore have sufficient storage available in the battery before entering the contract. Accordingly, the following problem can be formulated for the battery owner: determine the optimal time to enter the contract and, if necessary, the optimal time to discharge electricity before entering the contract. This problem is formulated as one of optimal stopping, and is solved explicitly in terms of the model parameters and instantaneous values of the power system imbalance. The optimal operational strategies thus obtained ensure that the battery owner has positive expected economic profit from the contract. Furthermore, they provide explicit conditions under which the optimal discharge time is consistent with the overall objective of power system balancing. This paper also carries out a preliminary investigation of the 'lifetime value' aggregated from an infinite sequence of these balancing reserve contracts. This lifetime value, which can be viewed as a single project valuation of the battery, is shown to be positive and bounded. Therefore, in the long run such reserve contracts can be beneficial to commercial operators of electricity storage, while reducing some of the financial and operational risks in power system balancing.This article is part of the themed issue 'Energy management: flexibility, risk and optimization'. © 2017 The Author(s).

  1. Policy instruments for regulating the development of wind power in a liberated electricity market

    Energy Technology Data Exchange (ETDEWEB)

    Morthorst, P.E. [Risoe National Lab., Roskilde (Denmark)

    1999-03-01

    Wind power is facing the dual challenge of entering a liberated electricity market and at the same time being one of the main contributors to the reduction of greenhouse gas emissions. The paper analyses the importance of the existing standard payment schemes in the development of wind power, and how this might be affected by the introduction of a liberated electricity market. The existing Danish standard payment scheme has strongly encouraged investments in wind turbines. It has been and still is very effective in promoting a high wind power capacity development, but at a high economic cost to the Danish Government. Different models of conditions for wind power at an electricity exchange do exist, but all seem to introduce a higher risk to the individual wind turbine owner than seen with the present payment scheme. In short it might be stated that going from the existing standard payment system to a market based system, the political uncertainty is converted to a market risk for the individual wind turbine owner. (au)

  2. Targeted deletion of RANKL in M cell inducer cells by the Col6a1-Cre driver.

    Science.gov (United States)

    Nagashima, Kazuki; Sawa, Shinichiro; Nitta, Takeshi; Prados, Alejandro; Koliaraki, Vasiliki; Kollias, George; Nakashima, Tomoki; Takayanagi, Hiroshi

    2017-11-04

    The gut-associated lymphoid tissues (GALTs), including Peyer's patches (PPs), cryptopatches (CPs) and isolated lymphoid follicles (ILFs), establish a host-microbe symbiosis by the promotion of immune reactions against gut microbes. Microfold cell inducer (MCi) cells in GALTs are the recently identified mesenchymal cells that express the cytokine RANKL and initiate bacteria-specific immunoglobulin A (IgA) production via induction of microfold (M) cell differentiation. In the previous study, the Twist2-Cre driver was utilized for gene deletion in mesenchymal cells including MCi cells. In order to investigate MCi cells more extensively, it will be necessary to develop experimental tools in addition to the Twist2-Cre driver mice and characterize such drivers in specificity and efficiency. Here we show that M cell differentiation and IgA production are impaired in the targeted deletion of RANKL by the Col6a1-Cre driver. We compared Col6a1-Cre with Twist2-Cre in terms of the specificity for mesenchymal cells in GALTs. Col6a1-Cre CAG-CAT-EGFP mice exhibited EGFP expression in podoplanin+CD31- cells including MCi cells, while Twist2-Cre mice were shown to target endothelial cells and podoplanin+CD31- cells. Tnfsf11fl/ΔCol6a1-Cre mice exhibited the absence of M cells and severe IgA reduction together with an alteration in gut microbial composition. Moreover, we analyzed germ free mice to test whether changes in the microbiota are the cause of M cell deficiency. M cell differentiation was normal in the CPs/ILFs of germ free mice, indicating that MCi cells induce M cells independently of microbial colonization. This study demonstrates that Col6a1-Cre driver mice are as useful as Twist2-Cre driver mice for functional analyses of GALT-resident mesenchymal cells, including MCi cells. Copyright © 2017 Elsevier Inc. All rights reserved.

  3. THEORETICAL ASPECTS OF THE PREDICTIONAL INSTRUMENTATION FOR APPLICATION IN THE STATE REGULATION OF THE PARTICIPANTS RELATIONSHIPS IN THE ELECTRICITY MARKET

    Directory of Open Access Journals (Sweden)

    Anastasiia Koliesnichenko

    2017-11-01

    Full Text Available The article presents the problems of current trends in the development of the electric power industry in conditions of increasing capital concentration, informing the economy and increasing the dynamics of the movement of significant amounts of cash flows which require a permanent analysis of the current situation and the necessary adjustment and / or modification of the parameters for the regulation of energy markets. From this article it can be concluded that the task of constructing forecasts acquires high relevance in many subject areas. It is an integral component of the daily work of modern social and economical systems. The interrelations of the subjects of the electric energy market are the one of the its most important institutions. The purpose of this article is to study the theoretical basis for the adaptive application of forecasting methods in the functioning of energy markets. In order to avoid the dominance of commercial interests of certain groups of participants in the energy market, the emergence of price distortions in the market that have a destructive effect on obtaining potential benefits from the introduction of competitive mechanisms, the key to finding the most effective and economical way to solve these problems is the use of systemic regulation of emerging deviations in achieving target milestones especially in the context of reform. The informational and analytical support formation for the performance of the functions assigned to the regulatory apparatus requires the use of forecasting methods and approaches to preserve the dynamic development of the electric energy market and to maintain the balance of interests of all its subjects in the conditions of reform. The article uses a number of methods: classification and systematization in the analysis of scientific methods for forecasting and planning social and economic processes, logical generalization when reviewing existing modeling methods and assessing their

  4. Electric power regulation in Italy a critical review; La regolazione elettrica in Italia. Una prima revisione critica

    Energy Technology Data Exchange (ETDEWEB)

    Clo, A. [Bologna Univ., Bologna (Italy)

    2000-12-01

    So far the results of the electricity reform in April 1999 have fallen short of expectations, since the spaces for free market and competition are still very thin in our power system. The regulator's activity has to be appraised within these spaces. An early, though difficult and approximate, analysis indicates that there are at least two major objections to the Electric Authority's activity: the excessive pervasiveness of regulation and the long times required for its implementation. [Italian] La riforma elettrica dell'aprile 1999 ha sortito sinora risultati ben lontani da quelli attesi, creando solo esigui spazi di libero mercato e di concorrenza nel nostro sistema elettrico. All'interno di questi spazi, come valutare l'operato del regolatore? Da un primo, seppur difficoltoso e approssimativo, bilancio paiono potersi muovere almeno due principali critiche all'operato dell'Autorita' elettrica: l'eccesso di pervasivita' della regolazione e la lunghezza dei tempi di attuazione della stessa.

  5. The Brazilian electric sector regulation as reference for the hydrogen regulation as energetic vector; A regulacao do setor eletrico brasileiro como referencia para regulacao do hidrogenio como vetor energetico

    Energy Technology Data Exchange (ETDEWEB)

    Paternostro, Andre de Goes [Universidade Salvador (UNIFACS), Salvador, BA (Brazil); Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPSB), Salvador, BA (Brazil)]. E-mail: apater@hotmail.com

    2008-07-01

    This paper is based on the Brazilian electric sector to outline parallels for introduction of hydrogen as energetic vector in Brazilian matrix. For this purpose it was achieved an analysis of regulation theory, of electric sector reorganization, the new model of energy trade and the NOS role. In face of this analysis, hydrogen regulation may be established through a mix of four models: tariff model by return tax; tariff by marginal cost; regulation by performance and price cap. This new industrial sector should start vertical and with its maturity become non vertical as electric sector after its reorganization. Regarding the market, advanced purchase of hydrogen should be established enabling the reduction of incertitude in trade as well as reduction of implantation costs. NOS should pursue electric energy generation from hydrogen as this may carry impacts in transmission networks as well as in distribution network depending on how hydrogen was generated, whether in large scale or for local consumption supply. (author)

  6. Two essays on electricity markets: Entry into hydroelectric generation industry and the political cycle of regulated prices

    Science.gov (United States)

    Moita, Rodrigo Menon Simoes

    This dissertation is about the electricity industry and the problems that arise with the liberalization and de-regulation of the industry. Characteristics intrinsic to the electricity market create problems that can compromise an efficient functioning of this market. Each of the two chapters of this dissertation focus on a specific aspect of this industry. The first chapter analyzes entry in the hydroelectric generation industry. The operation of a generator upstream regularizes the river flow for generators located downstream on the same river, increasing the production capacity of the latter. This positive externality increases the attractiveness of the locations downstream whenever a generator decides to enter upstream. Therefore, the entry decision of a generator in a given location may affect all entry decisions in potential locations for plants located downstream. I first model the problem of generators located in cascade on the same river and show the positive effect of the externality. Second, I use a panel of data on investment decisions of hydro-generation firms to estimate an entry model that takes into account the effect of the externality generated by entry upriver. The results show a positive incentive to locate downstream from existing plants and from locations where entry is likely to occur. Location characteristics also play an important role on the entrants' decisions. The model provides estimates of the average expected market price across the different years covered by the sample and shows that it rose one year before the energy crisis of 2001, evidencing that the market anticipated the crisis. This result has important implications on the evaluation of the Brazilian market design. It shows that entry responded to a rise in expectations about excess demand in the future, contradicting the argument that the crisis was a consequence of mis-designed market institutions. The second chapter deals with the problem of the political cycle in regulated

  7. Consultation about the information on electricity grid users; Consultation sur l'information des utilisateurs du Reseau de Transport d'Electricite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    The French power transmission system operator (RTE) is in charge of managing the power flows over its network and of managing the interconnections with foreign transmission networks. The performance of these assignments requires the dissemination of information by RTE for grid users. The French commission of electricity regulation (CRE) has decided to organize a consultation of network users concerning the publication by RTE of information about the state of the network. This document presents the questions of this consultation in the domain of: grid operation and users' information (congestions, network flows), purchases and allocations (interconnection capacities, compensation of losses), publication of proposals and results about purchases and allocations. (J.S.)

  8. Proposal of law relative to the electricity and natural gas regulated prices; Proposition de loi relative aux tarifs reglementes d'electricite et de gaz naturel

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-01-15

    This proposal of law relative to regulated electricity and gas tariffs, concerns one important modification of the article 66 from the law no 2005-781 of July 13, 2005 about the main guidelines of the French energy policy. This modification allows the households who have made the choice of a de-regulated energy supplier to change back and benefit again of the regulated tariffs. This document presents the items added in this way to the article 66. (J.S.)

  9. Tamoxifen-Induced Cre-loxP Recombination Is Prolonged in Pancreatic Islets of Adult Mice.

    Directory of Open Access Journals (Sweden)

    Rachel B Reinert

    Full Text Available Tamoxifen (Tm-inducible Cre recombinases are widely used to perform gene inactivation and lineage tracing studies in mice. Although the efficiency of inducible Cre-loxP recombination can be easily evaluated with reporter strains, the precise length of time that Tm induces nuclear translocation of CreER(Tm and subsequent recombination of a target allele is not well defined, and difficult to assess. To better understand the timeline of Tm activity in vivo, we developed a bioassay in which pancreatic islets with a Tm-inducible reporter (from Pdx1(PB-CreER(Tm;R26R(lacZ mice were transplanted beneath the renal capsule of adult mice previously treated with three doses of 1 mg Tm, 8 mg Tm, or corn oil vehicle. Surprisingly, recombination in islet grafts, as assessed by expression of the β-galactosidase (β-gal reporter, was observed days or weeks after Tm treatment, in a dose-dependent manner. Substantial recombination occurred in islet grafts long after administration of 3×8 mg Tm: in grafts transplanted 48 hours after the last Tm injection, 77.9±0.4% of β-cells were β-gal+; in β-cells placed after 1 week, 46.2±5.0% were β-gal+; after 2 weeks, 26.3±7.0% were β-gal+; and after 4 weeks, 1.9±0.9% were β-gal+. Islet grafts from mice given 3×1 mg Tm showed lower, but notable, recombination 48 hours (4.9±1.7% and 1 week (4.5±1.9% after Tm administration. These results show that Tm doses commonly used to induce Cre-loxP recombination may continue to label significant numbers of cells for weeks after Tm treatment, possibly confounding the interpretation of time-sensitive studies using Tm-dependent models. Therefore, investigators developing experimental approaches using Tm-inducible systems should consider both maximal recombination efficiency and the length of time that Tm-induced Cre-loxP recombination occurs.

  10. 5-HT inhibition of rat insulin 2 promoter Cre recombinase transgene and proopiomelanocortin neuron excitability in the mouse arcuate nucleus.

    Science.gov (United States)

    Hisadome, K; Smith, M A; Choudhury, A I; Claret, M; Withers, D J; Ashford, M L J

    2009-03-03

    A number of anti-obesity agents have been developed that enhance hypothalamic 5-HT transmission. Various studies have demonstrated that arcuate neurons, which express proopiomelanocortin peptides (POMC neurons), and neuropeptide Y with agouti-related protein (NPY/AgRP) neurons, are components of the hypothalamic circuits responsible for energy homeostasis. An additional arcuate neuron population, rat insulin 2 promoter Cre recombinase transgene (RIPCre) neurons, has recently been implicated in hypothalamic melanocortin circuits involved in energy balance. It is currently unclear how 5-HT modifies neuron excitability in these local arcuate neuronal circuits. We show that 5-HT alters the excitability of the majority of mouse arcuate RIPCre neurons, by either hyperpolarization and inhibition or depolarization and excitation. RIPCre neurons sensitive to 5-HT, predominantly exhibit hyperpolarization and pharmacological studies indicate that inhibition of neuronal firing is likely to be through 5-HT(1F) receptors increasing current through a voltage-dependent potassium conductance. Indeed, 5-HT(1F) receptor immunoreactivity co-localizes with RIPCre green fluorescent protein expression. A minority population of POMC neurons also respond to 5-HT by hyperpolarization, and this appears to be mediated by the same receptor-channel mechanism. As neither POMC nor RIPCre neuronal populations display a common electrical response to 5-HT, this may indicate that sub-divisions of POMC and RIPCre neurons exist, perhaps serving different outputs.

  11. Electrical stimulation of dog pudendal nerve regulates the excitatory pudendal-to-bladder reflex

    Directory of Open Access Journals (Sweden)

    Yan-he Ju

    2016-01-01

    Full Text Available Pudendal nerve plays an important role in urine storage and voiding. Our hypothesis is that a neuroprosthetic device placed in the pudendal nerve trunk can modulate bladder function after suprasacral spinal cord injury. We had confirmed the inhibitory pudendal-to-bladder reflex by stimulating either the branch or the trunk of the pudendal nerve. This study explored the excitatory pudendal-to-bladder reflex in beagle dogs, with intact or injured spinal cord, by electrical stimulation of the pudendal nerve trunk. The optimal stimulation frequency was approximately 15-25 Hz. This excitatory effect was dependent to some extent on the bladder volume. We conclude that stimulation of the pudendal nerve trunk is a promising method to modulate bladder function.

  12. The effect of CreA in glucose and xylose catabolism in Aspergillus nidulans

    DEFF Research Database (Denmark)

    Prathumpai, Wai; Mcintyre, Mhairi; Nielsen, Jens

    2004-01-01

    The catabolism of glucose and xylose was studied in a wild type and creA deleted (carbon catabolite de-repressed) strain of Aspergillus nidulans. Both strains were cultivated in bioreactors with either glucose or xylose as the sole carbon source, or in the presence of both sugars....... In the cultivations on single carbon sources, it was demonstrated that xylose acted as a carbon catabolite repressor (xylose cultivations), while the enzymes in the xylose utilisation pathway were also subject to repression in the presence of glucose (glucose cultivations). In the wild type strain growing...... on the sugar mixture, glucose repression of xylose utilisation was observed; with xylose utilisation occurring only after glucose was depleted. This phenomenon was not seen in the creA deleted strain, where glucose and xylose were catabolised simultaneously. Measurement of key metabolites and the activities...

  13. Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish

    Science.gov (United States)

    Mosimann, Christian; Kaufman, Charles K.; Li, Pulin; Pugach, Emily K.; Tamplin, Owen J.; Zon, Leonard I.

    2011-01-01

    Molecular genetics approaches in zebrafish research are hampered by the lack of a ubiquitous transgene driver element that is active at all developmental stages. Here, we report the isolation and characterization of the zebrafish ubiquitin (ubi) promoter, which drives constitutive transgene expression during all developmental stages and analyzed adult organs. Notably, ubi expresses in all blood cell lineages, and we demonstrate the application of ubi-driven fluorophore transgenics in hematopoietic transplantation experiments to assess true multilineage potential of engrafted cells. We further generated transgenic zebrafish that express ubiquitous 4-hydroxytamoxifen-controlled Cre recombinase activity from a ubi:creERt2 transgene, as well as ubi:loxP-EGFP-loxP-mCherry (ubi:Switch) transgenics and show their use as a constitutive fluorescent lineage tracing reagent. The ubi promoter and the transgenic lines presented here thus provide a broad resource and important advancement for transgenic applications in zebrafish. PMID:21138979

  14. Tracing notochord-derived cells using a Noto-cre mouse: implications for intervertebral disc development

    Directory of Open Access Journals (Sweden)

    Matthew R. McCann

    2012-01-01

    Back pain related to intervertebral disc degeneration is the most common musculoskeletal problem, with a lifetime prevalence of 82%. The lack of effective treatment for this widespread problem is directly related to our limited understanding of disc development, maintenance and degeneration. The aim of this study was to determine the developmental origins of nucleus pulposus cells within the intervertebral disc using a novel notochord-specific Cre mouse. To trace the fate of notochordal cells within the intervertebral disc, we derived a notochord-specific Cre mouse line by targeting the homeobox gene Noto. Expression of this gene is restricted to the node and the posterior notochord during gastrulation [embryonic day 7.5 (E7.5-E12.5]. The Noto-cre mice were crossed with a conditional lacZ reporter for visualization of notochord fate in whole-mount embryos. We performed lineage-tracing experiments to examine the contribution of the notochord to spinal development from E12.5 through to skeletally mature mice (9 months. Fate mapping studies demonstrated that, following elongation and formation of the primitive axial skeleton, the notochord gives rise to the nucleus pulposus in fully formed intervertebral discs. Cellular localization of β-galactosidase (encoded by lacZ and cytokeratin-8 demonstrated that both notochordal cells and chondrocyte-like nucleus pulposus cells are derived from the embryonic notochord. These studies establish conclusively that notochordal cells act as embryonic precursors to all cells found within the nucleus pulposus of the mature intervertebral disc. This suggests that notochordal cells might serve as tissue-specific progenitor cells within the disc and establishes the Noto-cre mouse as a unique tool to interrogate the contribution of notochordal cells to both intervertebral disc development and disc degeneration.

  15. Being a Learner Using Social Media in School: The Case of Space2cre8

    Science.gov (United States)

    Vasbø, Kristin Beate; Silseth, Kenneth; Erstad, Ola

    2014-01-01

    The aim of this article is to gain knowledge about what it means to be a learner using social media in an educational setting. The article presents an ethnographic study of students in a multiethnic community in Oslo who participate in a social networking site called Space2cre8 (S28). In this article, we set out to explore the kind of space for…

  16. Utilizing the CreA Gene to Better Understand Carbon Flux in Fungi

    Energy Technology Data Exchange (ETDEWEB)

    Annecharico, Matthew D.; Baker, Scott E.

    2005-01-01

    Carbon catabolite repression (CCR) is a system which represses the synthesis of enzymes required for certain carbon sources when more favored carbon sources are present. Fungal enzymes are useful in various industrial applications such as the degradation of bi-product biomass produced in many areas of manufacturing and production. For example, the basidiomycete, Phanerochaete chrysosporium, a filamentous fungus, produces a number of lignin peroxidases (LiPs) such as an extra-cellular lignin-modifying protein and manganese-dependent peroxidase (MnPs). The LiPs secreted by P. chrysosporium are able to break down lignin, one of the main substances of wood, while leaving the cellulose behind. These enzymes are capable of degrading toxic waste as well as biomass waste. Through polysaccharide hydrolysis and lignin utilization, biobased products and biofuels can be processed from biomass instead of petroleum, which is more environmentally sound than current fuel options. Previous research in the fungal phylum, Ascomyces has shown that the creA gene is an important gene that controls various enzymes which have key roles in controlling carbon utilization by fungi. We hypothesize that there are similarities across all fungi in the mechanism by which carbon flux is controlled and that orthologs of the creA gene exist in all fungal phyla. A COnsensus-DEgenerate Hybrid Oligonucleotide Primers (CODEHOP) polymerase chain reaction (PCR) strategy was used to isolate genomic fragments of the creA gene from a variety of fungi with the objective to design and utilize a method for isolation of the creA gene from a wide range of fungi.

  17. Highly efficient sex chromosome interchanges produced by I-CreI expression in Drosophila.

    Science.gov (United States)

    Maggert, Keith A; Golic, Kent G

    2005-11-01

    The homing endonuclease I-CreI recognizes a site in the gene encoding the 23S rRNA of Chlamydomonas reinhardtii. A very similar sequence is present in the 28S rRNA genes that are located on the X and Y chromosomes of Drosophila melanogaster. In this work we show that I-CreI expression in Drosophila is capable of causing induced DNA damage and eliciting cell cycle arrest. Expression also caused recombination between the X and Y chromosomes in the heterochromatic regions where the rDNA is located, presumably as a result of a high frequency of double-strand breaks in these regions. Approximately 20% of the offspring of males expressing I-CreI showed exceptional inheritance of X- and Y-linked markers, consistent with chromosome exchange at rDNA loci. Cytogenetic analysis confirmed the structures of many of these products. Exchange between the X and Y chromosomes can be induced in males and females to produce derivative-altered Y chromosomes, attached-XY, and attached-X chromosomes. This method has advantages over the traditional use of X rays for generating X-Y interchanges because it is very frequent and it generates predictable products.

  18. Generation of eGFP and Cre knockin rats by CRISPR/Cas9.

    Science.gov (United States)

    Ma, Yuanwu; Ma, Jing; Zhang, Xu; Chen, Wei; Yu, Lei; Lu, Yingdong; Bai, Lin; Shen, Bin; Huang, Xingxu; Zhang, Lianfeng

    2014-09-01

    The type II bacterial CRISPR/Cas [clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas)] system is a very valuable genome engineering tool, which has been widely used in genome editing of a variety of organisms. Previously, we generated floxed alleles in rats by CRISPR/Cas9. Here, we successfully use a two-cut strategy with one circular vector, which contains the exogenous cDNAs with homology arm regions, in generating knockin rats at the Trdmt1, Nestin and Cck loci. The efficiency of CRISPR/Cas9-mediated knockin was up to 54%. Furthermore, by crossing the Nestin-Cre rat with the Dnmt3b floxed rat and Cck-Cre with the Dnmt1 floxed rat, we detected Cre/loxP-mediated recombination in the F1 generation of rats. We also show that the knockin alleles were germline transmitted. These results provided a simple and flexible engineering strategy for the establishment of knockin rats. © 2014 FEBS.

  19. All-in-one construct for genome engineering using Cre-lox technology.

    Science.gov (United States)

    Mariscal, Ana M; González-González, Luis; Querol, Enrique; Piñol, Jaume

    2016-06-01

    Mycoplasma genitalium is an appealing model of a minimal cell and synthetic biology study, and it was one of the first organisms whose genome was fully sequenced and chemically synthesized. Despite its usefulness as a model organism, many genetic tools well established for other microorganisms are not currently available in mycoplasmas. We have developed several vectors to adapt the Cre-lox technology for genome engineering in M. genitalium, providing an all-in-one construct that could be also useful to obtain unmarked genetic modifications in many other slow growing microorganisms. This construct contains a modified promoter sequence based in TetR system that exhibits an enhanced control on Cre recombinase expression, virtually abolishing the presence of this recombinase in the absence of inducer. This allows to introduce the Cre recombinase gene and the desired genetic modification in a single transformation step. In addition, this inducible promoter may be a very promising tool for a wide range of molecular applications. © The Author 2016. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

  20. Strategies of diversification in regulated energy markets: the natural gas and electric power industries in Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Bazan Gonzalez, Gerado; Elizalde Baltierra, Alberto; Eibenschutz Hartman, Juan

    2007-07-01

    The aim of this work is to show a structured and systematic approach to analyze the strategies of diversification undertaken by the State-owned companies Petroleos Mexicanos and Comision Federal de Electricidad, during the period 1938-2006, in the Mexican energy market that has been strongly dominated by the State control. Furthermore, we explore the future of these strategies by using a Strengthens, Weaknesses, Opportunities and Threats (SWOT) matrix. This analyze also include changes in regulation that should happen in order to consolidated the proposed strategies. (auth)

  1. Reactions of Cre with methylphosphonate DNA: similarities and contrasts with Flp and vaccinia topoisomerase.

    Directory of Open Access Journals (Sweden)

    Chien-Hui Ma

    2009-09-01

    Full Text Available Reactions of vaccinia topoisomerase and the tyrosine site-specific recombinase Flp with methylphosphonate (MeP substituted DNA substrates, have provided important insights into the electrostatic features of the strand cleavage and strand joining steps catalyzed by them. A conserved arginine residue in the catalytic pentad, Arg-223 in topoisomerase and Arg-308 in Flp, is not essential for stabilizing the MeP transition state. Topoisomerase or its R223A variant promotes cleavage of the MeP bond by the active site nucleophile Tyr-274, followed by the rapid hydrolysis of the MeP-tyrosyl intermediate. Flp(R308A, but not wild type Flp, mediates direct hydrolysis of the activated MeP bond. These findings are consistent with a potential role for phosphate electrostatics and active site electrostatics in protecting DNA relaxation and site-specific recombination, respectively, against abortive hydrolysis.We have examined the effects of DNA containing MeP substitution in the Flp related Cre recombination system. Neutralizing the negative charge at the scissile position does not render the tyrosyl intermediate formed by Cre susceptible to rapid hydrolysis. Furthermore, combining the active site R292A mutation in Cre (equivalent to the R223A and R308A mutations in topoisomerase and Flp, respectively with MeP substitution does not lead to direct hydrolysis of the scissile MeP bond in DNA. Whereas Cre follows the topoisomerase paradigm during the strand cleavage step, it follows the Flp paradigm during the strand joining step.Collectively, the Cre, Flp and topoisomerase results highlight the contribution of conserved electrostatic complementarity between substrate and active site towards transition state stabilization during site-specific recombination and DNA relaxation. They have potential implications for how transesterification reactions in nucleic acids are protected against undesirable abortive side reactions. Such protective mechanisms are significant

  2. Transcutaneous electrical nerve stimulation regulates organ blood flow and apoptosis during controlled hypotension in dogs.

    Directory of Open Access Journals (Sweden)

    Lele Zhang

    Full Text Available Transcutaneous electrical nerve stimulation (TENS is commonly used in clinical practice for alleviating pains and physiological disorders. It has been reported that TENS could counteract the ischemic injury happened in some vital organs. To determine the protective effect of TENS on internal organs during CH in dogs, target hypotension was maintained for 60 min at 50% of the baseline mean arterial pressure (MAP. The perfusion to the brain, liver, stomach, and kidney was recorded and apoptosis within these organs was observed. Results showed that when arriving at the target MAP, and during the maintaining stage for 10 min, perfusion to the stomach and liver in the CH+TENS group was much higher than in the CH group (P<0.05. Perfusion to the cerebral cortex greatly declined in both the controlled pressure groups when compared with the general anesthesia (GA group (P<0.05. After withdrawing CH, the hepatic blood flow in both the CH and CH+TENS groups, and the gastric and cerebral cortical blood flow in the CH+TENS group, were rapidly increased. By the end of MAP restoration, gastric blood flow in the CH group was still low. At 72 h after applying CH, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL-positive cells in stomach and kidney tissue from the CH group were significantly increased compared with those in the GA group (P<0.05. There was no significant difference in TUNEL-positive cells in the liver and hippocampus among the three groups. Our results demonstrated that CH with a 50% MAP level could cause lower perfusion to the liver, stomach, cerebral cortex, and kidney, with apoptosis subsequently occurring in the stomach and kidney. TENS combined GA is able to improve the blood flow to the liver, stomach, and reduce the apoptosis in the stomach and kidney.

  3. A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination.

    Science.gov (United States)

    Imayoshi, Itaru; Hirano, Kyoko; Sakamoto, Masayuki; Miyoshi, Goichi; Imura, Tetsuya; Kitano, Satsuki; Miyachi, Hitoshi; Kageyama, Ryoichiro

    2012-05-01

    Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent protein) after Cre- and Flp-mediated recombination. To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. The germline excision of the Frt-flanked stop cassette in R26-CAG-LF-mTFP1 generated a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the loxP-flanked stop cassette was excised in the germline, requires only Flp to activate mTFP1 expression. Copyright © 2012 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

  4. Transcutaneous electrical nerve stimulation (TENS) improves the diabetic cytopathy (DCP) via up-regulation of CGRP and cAMP.

    Science.gov (United States)

    Ding, Liucheng; Song, Tao; Yi, Chaoran; Huang, Yi; Yu, Wen; Ling, Lin; Dai, Yutian; Wei, Zhongqing

    2013-01-01

    The objective of this study was to investigate the effects and mechanism of Transcutaneous Electrical Nerve Stimulation (TENS) on the diabetic cytopathy (DCP) in the diabetic bladder. A total of 45 rats were randomly divided into diabetes mellitus (DM)/TENS group (n=15), DM group (n=15) and control group (n=15). The rats in the DM/TENS and TENS groups were electronically stimulated (stimulating parameters: intensity-31 V, frequency-31 Hz, and duration of stimulation of 15 min) for three weeks. Bladder histology, urodynamics and contractile responses to field stimulation and carbachol were determined. The expression of calcitonin gene-related peptide (CGRP) was analyzed by RT-PCR and Western blotting. The results showed that contractile responses of the DM rats were ameliorated after 3 weeks of TENS. Furthermore, TENS significantly increased bladder wet weight, volume threshold for micturition and reduced PVR, V% and cAMP content of the bladder. The mRNA and protein levels of CGRP in dorsal root ganglion (DRG) in the DM/TENS group were higher than those in the DM group. TENS also significantly up-regulated the cAMP content in the bladder body and base compared with diabetic rats. We conclude that TENS can significantly improve the urine contractility and ameliorate the feeling of bladder fullness in DM rats possibly via up-regulation of cAMP and CGRP in DRG.

  5. Temporal control of gene deletion in sensory ganglia using a tamoxifen-inducible Advillin-Cre-ERT2 recombinase mouse

    Directory of Open Access Journals (Sweden)

    Lau Joanne

    2011-12-01

    Full Text Available Abstract Background Tissue-specific gene deletion has proved informative in the analysis of pain pathways. Advillin has been shown to be a pan-neuronal marker of spinal and cranial sensory ganglia. We generated BAC transgenic mice using the Advillin promoter to drive a tamoxifen-inducible CreERT2 recombinase construct in order to be able to delete genes in adult animals. We used a floxed stop ROSA26LacZ reporter mouse to examine functional Cre expression, and analysed the behaviour of mice expressing Cre recombinase. Results We used recombineering to introduce a CreERT2 cassette in place of exon 2 of the Advillin gene into a BAC clone (RPCI23-424F19 containing the 5' region of the Advillin gene. Transgenic mice were generated using pronuclear injection. The resulting AvCreERT2 transgenic mice showed a highly specific expression pattern of Cre activity after tamoxifen induction. Recombinase activity was confined to sensory neurons and no expression was found in other organs. Less than 1% of neurons showed Cre expression in the absence of tamoxifen treatment. Five-day intraperitoneal treatment with tamoxifen (2 mg per day induced Cre recombination events in ≈90% of neurons in dorsal root and cranial ganglia. Cell counts of dorsal root ganglia (DRG from transgenic animals with or without tamoxifen treatment showed no neuronal cell loss. Sensory neurons in culture showed ≈70% induction after 3 days treatment with tamoxifen. Behavioural tests showed no differences between wildtype, AvCreERT2 and tamoxifen-treated animals in terms of motor function, responses to light touch and noxious pressure, thermal thresholds as well as responses to inflammatory agents. Conclusions Our results suggest that the inducible pan-DRG AvCreERT2 deleter mouse strain is a useful tool for studying the role of individual genes in adult sensory neuron function. The pain phenotype of the Cre-induced animal is normal; therefore any alterations in pain processing can be

  6. Establishment of a Cre/loxP recombination system for N-terminal epitope tagging of genes in Tetrahymena

    OpenAIRE

    Mochizuki Kazufumi; Vogt Alexander; Busch Clara

    2010-01-01

    Abstract Background Epitope tagging is a powerful strategy to study the function of proteins. Although tools for C-terminal protein tagging in the ciliated protozoan Tetrahymena thermophila have been developed, N-terminal protein tagging in this organism is still technically demanding. Results In this study, we have established a Cre/loxP recombination system in Tetrahymena and have applied this system for the N-terminal epitope tagging of Tetrahymena genes. Cre can be expressed in Tetrahymen...

  7. Reciprocal Regulation of Epileptiform Neuronal Oscillations and Electrical Synapses in the Rat Hippocampus

    Science.gov (United States)

    Kinjo, Erika R.; Higa, Guilherme S. V.; Morya, Edgard; Valle, Angela C.; Kihara, Alexandre H.; Britto, Luiz R. G.

    2014-01-01

    Gap junction (GJ) channels have been recognized as an important mechanism for synchronizing neuronal networks. Herein, we investigated the participation of GJ channels in the pilocarpine-induced status epilepticus (SE) by analyzing electrophysiological activity following the blockade of connexins (Cx)-mediated communication. In addition, we examined the regulation of gene expression, protein levels, phosphorylation profile and distribution of neuronal Cx36, Cx45 and glial Cx43 in the rat hippocampus during the acute and latent periods. Electrophysiological recordings revealed that the GJ blockade anticipates the occurrence of low voltage oscillations and promotes a marked reduction of power in all analyzed frequencies.Cx36 gene expression and protein levels remained stable in acute and latent periods, whereas upregulation of Cx45 gene expression and protein redistribution were detected in the latent period. We also observed upregulation of Cx43 mRNA levels followed by changes in the phosphorylation profile and protein accumulation. Taken together, our results indisputably revealed that GJ communication participates in the epileptiform activity induced by pilocarpine. Moreover, considering that specific Cxs undergo alterations through acute and latent periods, this study indicates that the control of GJ communication may represent a focus in reliable anti-epileptogenic strategies. PMID:25299405

  8. Environmental Regulations on Waste Electrical Equipment Industry: The Production Chain to the Final Consumer

    Directory of Open Access Journals (Sweden)

    Marilia Tunes Mazon

    2012-06-01

    Full Text Available The relevance of the electronics industry requires its transition to sustainable production patterns, as defined by the European directives WEEE, RoHS, and the National Policy on Solid Waste. In 2010, the group began a national project whose purpose is to support sustainability with a focus on the triple bottom line Brazilian electronics industry. The implementation of these proposals began with the implementation of a pilot project with nine companies of electromedical equipment, chosen because they are technology intensive, and require exporters suit environmental regulations. This paper aims to present the main results obtained in the studies with these nine companies in the period Apr/2011 Mar/2012 regarding the suitability of its products, processes, management systems and supply chains. The study was divided into three stages: an assessment to identify general aspects of each organization, a thorough assessment to understand the characteristics of each organization ahead of the general characteristics of the group analyzed and a detailed diagnosis, performed individually during visits in companies. The main findings were that the waste management and reverse logistics associated are still incipient, the products still have dangerous substances and supply chain is still very poorly prepared for the challenges. The level of employment of employees and costs associated with the adequacy of its products and processes, constitute the two biggest problems for companies fit the relevant environmental legislation.

  9. Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system.

    Science.gov (United States)

    Corneille, S; Lutz, K; Svab, Z; Maliga, P

    2001-07-01

    Incorporation of a selectable marker gene during transformation is essential to obtain transformed plastids. However, once transformation is accomplished, having the marker gene becomes undesirable. Here we report on adapting the P1 bacteriophage CRE-lox site-specific recombination system for the elimination of marker genes from the plastid genome. The system was tested by the elimination of a negative selectable marker, codA, which is flanked by two directly oriented lox sites (>codA>). Highly efficient elimination of >codA> was triggered by introduction of a nuclear-encoded plastid-targeted CRE by Agrobacterium transformation or via pollen. Excision of >codA> in tissue culture cells was frequently accompanied by a large deletion of a plastid genome segment which includes the tRNA-ValUAC gene. However, the large deletions were absent when cre was introduced by pollination. Thus pollination is our preferred protocol for the introduction of cre. Removal of the >codA> coding region occurred at a dramatic speed, in striking contrast to the slow and gradual build-up of transgenic copies during plastid transformation. The nuclear cre gene could subsequently be removed by segregation in the seed progeny. The modified CRE-lox system described here will be a highly efficient tool to obtain marker-free transplastomic plants.

  10. Restoring pollen fertility in transgenic male-sterile eggplant by Cre/loxp-mediated site-specific recombination system

    Directory of Open Access Journals (Sweden)

    Bihao Cao

    2010-01-01

    Full Text Available This study was designed to control plant fertility by cell lethal gene Barnase expressing at specific developmental stage and in specific tissue of male organ under the control of Cre/loxP system, for heterosis breeding, producing hybrid seed of eggplant. The Barnase-coding region was flanked by loxP recognition sites for Cre-recombinase. The eggplant inbred/pure line ('E-38' was transformed with Cre gene and the inbred/pure line ('E-8' was transformed with the Barnase gene situated between loxp. The experiments were done separately, by means of Agrobacterium co-culture. Four T0-plants with the Barnase gene were obtained, all proved to be male-sterile and incapable of producing viable pollen. Flowers stamens were shorter, but the vegetative phenotype was similar to wild-type. Five T0-plants with the Cre gene developed well, blossomed out and set fruit normally. The crossing of male-sterile Barnase-plants with Cre expression transgenic eggplants resulted in site-specific excision with the male-sterile plants producing normal fruits. With the Barnase was excised, pollen fertility was fully restored in the hybrids. The phenotype of these restored plants was the same as that of the wild-type. Thus, the Barnase and Cre genes were capable of stable inheritance and expression in progenies of transgenic plants.

  11. Generation of a Tlx1(CreER-Venus) knock-in mouse strain for the study of spleen development.

    Science.gov (United States)

    Nakahara, Ryo; Kawai, Yasuhiro; Oda, Akihisa; Nishimura, Miyuki; Murakami, Akikazu; Azuma, Takachika; Kaifu, Tomonori; Goitsuka, Ryo

    2014-11-01

    The spleen is a lymphoid organ that serves as a unique niche for immune reactions, extramedullary hematopoiesis, and the removal of aged erythrocytes from the circulation. While much is known about the immunological functions of the spleen, the mechanisms governing the development and organization of its stromal microenvironment remain poorly understood. Here we report the generation and analysis of a Tlx1(Cre) (ER) (-Venus) knock-in mouse strain engineered to simultaneously express tamoxifen-inducible CreER(T2) and Venus fluorescent protein under the control of regulatory elements of the Tlx1 gene, which encodes a transcription factor essential for spleen development. We demonstrated that Venus as well as CreER expression recapitulates endogenous Tlx1 transcription within the spleen microenvironment. When Tlx1(Cre) (ER) (-Venus) mice were crossed with the Cre-inducible reporter strain, Tlx1-expressing cells as well as their descendants were specifically labeled following tamoxifen administration. We also showed by cell lineage tracing that asplenia caused by Tlx1 deficiency is attributable to altered contribution of mesenchymal cells in the spleen anlage to the pancreatic mesenchyme. Thus, Tlx1(Cre) (ER) (-Venus) mice represent a new tool for lineage tracing and conditional gene manipulation of spleen mesenchymal cells, essential approaches for understanding the molecular mechanisms of spleen development. © 2014 Wiley Periodicals, Inc.

  12. The phenylquinazoline compound S-4893 is a non-competitive cytokinin antagonist that targets Arabidopsis cytokinin receptor CRE1 and promotes root growth in Arabidopsis and rice.

    Science.gov (United States)

    Arata, Yuto; Nagasawa-Iida, Asako; Uneme, Hideki; Nakajima, Hiroki; Kakimoto, Tatsuo; Sato, Ryo

    2010-12-01

    We identified two phenylquinazoline compounds in a large-scale screening for cytokinin antagonists in yeast expressing the Arabidopsis cytokinin receptor cytokinin response 1/histidine kinase 4 (CRE1). After chemical modifications, we obtained compound S-4893, which non-competitively inhibited binding of the natural ligand 2-isopentenyladenine to CRE1. S-4893 antagonized cytokinin-induced activation of the Arabidopsis response regulator 5 promoter in Arabidopsis. Importantly, S-4893 had no detectable intrinsic cytokinin agonist activity in Arabidopsis or in the transformed yeast system. Cytokinin bioassay further demonstrated that S-4893 antagonized cytokinin-induced stimulation of callus formation and inhibition of root elongation. S-4893 also promoted seminal, crown and lateral root growth in rice, suggesting that S-4893 could potentially promote root growth in a variety of agronomically important plants. We believe S-4893 will be a useful tool in functional studies of cytokinin action in a wide range of plants and a lead compound for the development of useful root growth promoters in agriculture.

  13. A good integrated resource plan: Guidelines for electric utilities and regulators

    Energy Technology Data Exchange (ETDEWEB)

    Hirst, E.

    1992-12-01

    Integrated resource planning helps utilities and state regulatory commissions consistently assess a broad range of demand and supply resources to meet customer energy-service needs cost-effectively. Key characteristics of this planning approach include: explicit consideration and fair treatment of a wide variety of demand and supply options, consideration of the environmental and other social costs of providing energy services, public participation in the development of the resource plan, and analysis of the uncertainties associated with different external factors and resource options. Integrated resource planning differs from traditional planning in the types and scope of resources considered, the owners of the resources, the organizations involved in resource planning, and the criteria for resource selection. This report presents suggestions to utilities on how to conduct such planning and what to include in their resource-planning reports. These suggestions are based on a review of about 50 resource plans as well as discussions with and presentations to regulators and utilities. The suggestions cover four broad topics; the technical competence with which the plan was developed; the adequacy, detail, and consistency (with the long-term plan) of the short-term action plan; the extent to which the interests of various stakeholders was considered, both in public participation in plan development and in the variety of resource plans developedand assessed; and the clarity and comprehensiveness of the utility`s report on its plan. Technical competence includes energy and demand forecasts, assessment of supply and demand resources, resource integration, and treatment of uncertainty. Issues associated with forecasts include forecasting approaches; links between the forecasts of energy use and peak demands; and links between the forecasts and the effects of past, present, and future demand-side management programs.

  14. A good integrated resource plan: Guidelines for electric utilities and regulators

    Energy Technology Data Exchange (ETDEWEB)

    Hirst, E.

    1992-12-01

    Integrated resource planning helps utilities and state regulatory commissions consistently assess a broad range of demand and supply resources to meet customer energy-service needs cost-effectively. Key characteristics of this planning approach include: explicit consideration and fair treatment of a wide variety of demand and supply options, consideration of the environmental and other social costs of providing energy services, public participation in the development of the resource plan, and analysis of the uncertainties associated with different external factors and resource options. Integrated resource planning differs from traditional planning in the types and scope of resources considered, the owners of the resources, the organizations involved in resource planning, and the criteria for resource selection. This report presents suggestions to utilities on how to conduct such planning and what to include in their resource-planning reports. These suggestions are based on a review of about 50 resource plans as well as discussions with and presentations to regulators and utilities. The suggestions cover four broad topics; the technical competence with which the plan was developed; the adequacy, detail, and consistency (with the long-term plan) of the short-term action plan; the extent to which the interests of various stakeholders was considered, both in public participation in plan development and in the variety of resource plans developedand assessed; and the clarity and comprehensiveness of the utility's report on its plan. Technical competence includes energy and demand forecasts, assessment of supply and demand resources, resource integration, and treatment of uncertainty. Issues associated with forecasts include forecasting approaches; links between the forecasts of energy use and peak demands; and links between the forecasts and the effects of past, present, and future demand-side management programs.

  15. Electrical contracting

    CERN Document Server

    Neidle, Michael

    2013-01-01

    Electrical Contracting, Second Edition is a nine-chapter text guide for the greater efficiency in planning and completing installations for the design, installation and control of electrical contracts. This book starts with a general overview of the efficient cabling and techniques that must be employed for safe wiring design, as well as the cost estimation of the complete electrical contract. The subsequent chapters are devoted to other electrical contracting requirements, including electronic motor control, lighting, and electricity tariffs. A chapter focuses on the IEE Wiring Regulations an

  16. The molecular biology of memory: cAMP, PKA, CRE, CREB-1, CREB-2, and CPEB

    Directory of Open Access Journals (Sweden)

    Kandel Eric R

    2012-05-01

    Full Text Available Abstract The analysis of the contributions to synaptic plasticity and memory of cAMP, PKA, CRE, CREB-1, CREB-2, and CPEB has recruited the efforts of many laboratories all over the world. These are six key steps in the molecular biological delineation of short-term memory and its conversion to long-term memory for both implicit (procedural and explicit (declarative memory. I here first trace the background for the clinical and behavioral studies of implicit memory that made a molecular biology of memory storage possible, and then detail the discovery and early history of these six molecular steps and their roles in explicit memory.

  17. The molecular biology of memory: cAMP, PKA, CRE, CREB-1, CREB-2, and CPEB.

    Science.gov (United States)

    Kandel, Eric R

    2012-05-14

    The analysis of the contributions to synaptic plasticity and memory of cAMP, PKA, CRE, CREB-1, CREB-2, and CPEB has recruited the efforts of many laboratories all over the world. These are six key steps in the molecular biological delineation of short-term memory and its conversion to long-term memory for both implicit (procedural) and explicit (declarative) memory. I here first trace the background for the clinical and behavioral studies of implicit memory that made a molecular biology of memory storage possible, and then detail the discovery and early history of these six molecular steps and their roles in explicit memory.

  18. Contribution for a history of the regulation of the electric energy sector in Brazil: the Water Code of 1934 and the Water and Electric Power National Council

    OpenAIRE

    Corrêa, Maria Letícia; Universidade do Estado do Rio de Janeiro

    2005-01-01

    This study aims to analyse the history of the Brazilian electric power sector, throughout the development of the Water and Electric Power National Council (CNAEE), between 1939 and 1954. The CNAEE was originally created to execute the Water Code — an legislative act conceived in 1934, which represented the consolidation of federal public authority concerning strategic resources, defining its competencies, responsibilities and rights —, but it also undertakes the assignment o...

  19. Development of Voltage Regulation Plan by Composing Subsystem with the SFES for DC On-line Electric Vehicle

    Science.gov (United States)

    Jung, S.; Lee, J. H.; Yoon, M.; Lee, H.; Jang, G.

    The study of the application process of the relatively small size 'Superconducting Flywheel Energy Storage (SFES)' system is conducted to regulate voltage fluctuation of the DC On-Line Electric Vehicle (OLEV) system, which is designed by using DC power system network. It is recommended to construct the power conversion system nearby the substation because the charging system is under the low voltage. But as the system is usually built around urban area and it makes hard to construct the subsystems at every station, voltage drop can occur in power supply inverter that is some distance from the substation. As the alternative of this issue, DC distribution system is recently introduced and has possibility to solve the above issue. In this paper, SFES is introduced to solve the voltage drop under the low voltage distribution system by using the concept of the proposed DC OLEV which results in building the longer distance power supply system. The simulation to design the SFES by using DC power flow analysis is carried out and it is verified in this paper.

  20. Federal regulations needing amendment to stimulate the production and introduction of electric/solar vehicles. A report to Congress, January 1990

    Energy Technology Data Exchange (ETDEWEB)

    1990-01-01

    The categories of federal regulations considered for the report were safety, emissions, and consumer protection. It was found that no current regulations act as a barrier to the introduction of electric vehicles (EVs) into commerce. The present stimulatory regulation, the Corporate Average Fuel Economy (CAFE) credit for EVs, is not sufficient to bring EVs into production, but should be maintained. The petroleum-equivalency factors in this regulation expired in 1987, but are currently being updated by the Department of Energy. There is a strong consensus among the various groups engaged in EV development and research, that automobile manufacturers retain the option to include equivalent petroleum-based fuel economy values for EVs in their CAFE (10 CFR Part 474), provided EVs are not used to determine the manufacturer's capability for purposes of establishing a fuel-economy standard. No future regulations which could be promulgated under standing legislations would be sufficient to accelerate the introduction of EVs.

  1. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain.

    Science.gov (United States)

    Deverman, Benjamin E; Pravdo, Piers L; Simpson, Bryan P; Kumar, Sripriya Ravindra; Chan, Ken Y; Banerjee, Abhik; Wu, Wei-Li; Yang, Bin; Huber, Nina; Pasca, Sergiu P; Gradinaru, Viviana

    2016-02-01

    Recombinant adeno-associated viruses (rAAVs) are commonly used vehicles for in vivo gene transfer. However, the tropism repertoire of naturally occurring AAVs is limited, prompting a search for novel AAV capsids with desired characteristics. Here we describe a capsid selection method, called Cre recombination-based AAV targeted evolution (CREATE), that enables the development of AAV capsids that more efficiently transduce defined Cre-expressing cell populations in vivo. We use CREATE to generate AAV variants that efficiently and widely transduce the adult mouse central nervous system (CNS) after intravenous injection. One variant, AAV-PHP.B, transfers genes throughout the CNS with an efficiency that is at least 40-fold greater than that of the current standard, AAV9 (refs. 14,15,16,17), and transduces the majority of astrocytes and neurons across multiple CNS regions. In vitro, it transduces human neurons and astrocytes more efficiently than does AAV9, demonstrating the potential of CREATE to produce customized AAV vectors for biomedical applications.

  2. Comparison of Three Cre-LoxP Based Paired-End Library Construction Methods

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Ze; Nath, Nandita; Tritt, Andrew; Liang, Shoudan; Han, James; Pennacchio, Len; Chen, Feng

    2013-03-26

    Paired-end library sequencing has been proven useful in scaffold construction during de novo whole genome shotgun assembly. The ability of generating mate pairs with > 8 Kb insert sizes is especially important for genomes containing long repeats. To make mate paired libraries for next generation sequencing, DNA fragments need to be circularized to bring the ends together. There are several methods that can be used for DNA circulation, namely ligation, hybridization and Cre-LoxP recombination. With higher circularization efficiency with large insert DNA fragments, Cre-LoxP recombination method generally has been used for constructing >8 kb insert size paired-end libraries. Second fragmentation step is also crucial for maintaining high library complexity and uniform genome coverage. Here we will describe the following three fragmentation methods: restriction enzyme digestion, random shearing and nick translation. We will present the comparison results for these three methods. Our data showed that all three methods are able to generate paired-end libraries with greater than 20 kb insert. Advantages and disadvantages of these three methods will be discussed as well.

  3. Agrobacterium-mediated transformation of maize (Zea mays) with Cre-lox site specific recombination cassettes in BIBAC vectors.

    Science.gov (United States)

    Vega, Juan M; Yu, Weichang; Han, Fangpu; Kato, Akio; Peters, Eric M; Zhang, Zhanyuan J; Birchler, James A

    2008-04-01

    The Cre/loxP site-specific recombination system has been applied in various plant species including maize (Zea mays) for marker gene removal, gene targeting, and functional genomics. A BIBAC vector system was adapted for maize transformation with a large fragment of genetic material including a herbicide resistance marker gene, a 30 kb yeast genomic fragment as a marker for fluorescence in situ hybridization (FISH), and a 35S-lox-cre recombination cassette. Seventy-five transgenic lines were generated from Agrobacterium-mediated transformation of a maize Hi II line with multiple B chromosomes. Eighty-four inserts have been localized among all 10 A chromosome pairs by FISH using the yeast DNA probe together with a karyotyping cocktail. No inserts were found on the B chromosomes; thus a bias against the B chromosomes by the Agrobacterium-mediated transformation was revealed. The expression of a cre gene was confirmed in 68 of the 75 transgenic lines by a reporter construct for cre/lox mediated recombination. The placement of the cre/lox site-specific recombination system in many locations in the maize genome will be valuable materials for gene targeting and chromosome engineering.

  4. Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector

    OpenAIRE

    Masato Ohtsuka; Takayuki Warita; Takayuki Sakurai; Satoshi Watanabe; Hidetoshi Inoko; Masahiro Sato

    2009-01-01

    We developed Cre-loxP-based systems, termed CRTEIL and CETRIZ, which allow gene switching in a noninvasive manner. Single transfection with pCRTEIL resulted in predominant expression of red fluorescence. Cotransfection with pCRTEIL and Cre-expression plasmid (pCAG/NCre) caused switching from red to green fluorescence. Similarly, cotransfection with pCETRIZ and pCAG/NCre resulted in change of green to red fluorescence. These noninvasive systems will be useful in cell lineage analysis, since de...

  5. The gas and electric power markets opening in Europe, the implementation of the regulation authorities; L'ouverture des marches du gaz et de l'electricite en Europe, la mise en place des instances de regulation

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-12-15

    Because the gas and the electric power are not simple goods, the opening of their market needs a whole reflexion where the governments must be involved. To analyse this opening, this report presents in a first part the traditional organization of the gas and electric power sectors marked by the strong presence of the government and the end of this organization at the end of the years 1980. It details then the new regulation, the operators and the effects of the competition. In a last part it provides propositions for the institutional framework in France. (A.L.B.)

  6. Establishment of a Cre/loxP recombination system for N-terminal epitope tagging of genes in Tetrahymena.

    Science.gov (United States)

    Busch, Clara Jana-Lui; Vogt, Alexander; Mochizuki, Kazufumi

    2010-07-13

    Epitope tagging is a powerful strategy to study the function of proteins. Although tools for C-terminal protein tagging in the ciliated protozoan Tetrahymena thermophila have been developed, N-terminal protein tagging in this organism is still technically demanding. In this study, we have established a Cre/loxP recombination system in Tetrahymena and have applied this system for the N-terminal epitope tagging of Tetrahymena genes. Cre can be expressed in Tetrahymena and localizes to the macronucleus where it induces precise recombination at two loxP sequences in direct orientation in the Tetrahymena macronuclear chromosome. This Cre/loxP recombination can be used to remove a loxP-flanked drug-resistance marker from an N-terminal tagging construct after it is integrated into the macronucleus. The system established in this study allows us to express an N-terminal epitope tagged gene from its own endogenous promoter in Tetrahymena.

  7. Learned EEG-based regulation of motor-related brain oscillations during application of transcranial electric currents: feasibility and limitations

    Directory of Open Access Journals (Sweden)

    Surjo R Soekadar

    2014-03-01

    Full Text Available Objective: Transcranial direct current stimulation (tDCS improves motor learning and can influence emotional processing or attention. However, it remained unclear whether learned electroencephalography (EEG-based brain-machine interface (BMI control during tDCS is feasible and how application of transcranial electric currents during BMI control would interfere with feature-extraction of physiological brain signals. Here we tested this combination and evaluated stimulation-dependent artifacts across different EEG frequencies and stability of motor imagery-based BMI control. Approach: Ten healthy volunteers were invited to two BMI-sessions, each comprising two 60-trial blocks. During the trials, modulation of mu-rhythms (8-15Hz associated with motor imagery recorded over C4 was translated into online cursor movements on a computer screen. During block 2, either sham (session A or anodal tDCS (session B was applied at 1mA with the stimulation electrode placed 1cm anterior of C4. Main results: tDCS was associated with a significant signal power increase in the lower frequencies most evident in the signal spectrum of the EEG channel closest to the stimulation electrode. Stimulation-dependent signal power increase exhibited a decay of 12dB per decade, leaving frequencies above 9Hz unaffected. Analysis of BMI control performance did not indicate a difference between blocks and tDCS conditions. Conclusion: Application of tDCS during learned EEG-based self-regulation of brain oscillations above 9Hz is feasible and safe, and might improve applicability of BMI systems in patient populations.

  8. The transient regulated tariff of the electricity market adjustment called 'return tariff'; Le tarif reglemente transitoire d'ajustement du marche de l'electricite dit 'tarif de retour'

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-01-15

    The transient electricity regulated tariff was implemented by the law of the 7 december 2006 relative to the energy sector. It offers an adapted response to the enterprises confronted with the electricity prices increase. The document details this regulated tariff, provides the corresponding order of the 3 january 2007 and an interpretative note on the implementation of the transient regulated tariff. (A.L.B.)

  9. No 3071. Resolution proposal aiming at creating an inquiry commission on the pricing conditions on the power market, and on their consequences for companies and consumers and on the necessity to implement regulation mechanisms; N. 3071. Proposition de resolution tendant a la creation d'une commission d'enquete sur les conditions de formation des prix sur le marche de l'electricite, sur leurs consequences pour les entreprises et les particuliers et sur la necessite de mettre en oeuvre des mecanismes de regulation,

    Energy Technology Data Exchange (ETDEWEB)

    Brottes, F.; Ayrault, J.M.; Bataille, Ch.; Ducout, P.; Emmanuelli, H.; Migaud, D.; Besson, E.; Bonrepaux, A.; Dumont, J.L.; Balligand, J.P

    2006-05-15

    Electricite de France (EdF), the French electric utility, announced on December 15, 2004 a rise of electricity prices and tariffs in order to finance EDF's development policy. About a year later, the electricity prices rise was much higher than expected and not justified neither by the financial health of the company, nor by any other particular reason. The only reason seems to be the classical logic that follows the privatization of any historical power operator: opening of the capital, reduction of manpower, and increase of prices. This document wonders about: the impact of prices rise on power consuming companies and on individual end-users, the real role of the commission of energy regulation (CRE) and on the reality of an energy market regulation. (J.S.)

  10. The estimated amount of the public utility costs for electricity production for 2002 is 3 euros / MWh; Le montant previsionnel des charges de service public de la production d'electricite pour 2002 sont de 3 euros / MWh

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    The estimated amount of the contribution to the public utility fund for electricity production (FSPPE) for 2002 is 3 euros/MWh, according to the order of January 25, 2002, following the proposal by the French electricity regulator (CRE) to the government on December 20, 2001. The FSPPE, which enables the financing of the public utility costs to be separated, is intended to finance the additional expenditure caused by the cogeneration and renewable energy support policies and the extra production costs in overseas departments and Corsica. The coming into force of the FSPPE will result in the simultaneous reduction of the provisional access tariff to the network, established by Electricite de France (EdF) in 1999, and to a marked average increase of electricity costs for eligible and non-eligible customers. CRE asked for a separate mention of public utility policy costs and supply costs on EdF's bills. Some technical files are attached to this press release and describe the operation of the FSPPE fund, the calculation methods used, the public consultation on the costs saved, and the achievement of the target on supporting the renewable energies at the least cost. (J.S.)

  11. Efficient Construction of Large Genomic Deletion in Agrobacterium tumefaciens by Combination of Cre/loxP System and Triple Recombineering.

    Science.gov (United States)

    Liu, Zhengqiang; Xie, Yali; Zhang, Xu; Hu, Xiaofeng; Li, Yusheng; Ding, Xuezhi; Xia, Liqiu; Hu, Shengbiao

    2016-04-01

    In order to develop an efficient system for deleting genomic segment in Agrobacterium tumefaciens to analyze gene functions and construct marker gene-free recombinant strains, a Cre recombinase expression plasmid was constructed by placing its encoding gene under the control of Ptet promoter and cloning into the plasmid replicable in both A. tumefaciens and E. coli. Triple recombineering was applied to efficiently construct integrative vectors which were used to introduce loxP sites and selection markers into the chromosome of A. tumefaciens. Cre recombinase could be properly induced by anhydrotetracycline in A. tumefaciens, which was revealed by the fact that kanamycin resistance gene flanked by two parallel loxP sites was excised from the genome of A. tumefaciens with virtually 100% efficiency. And what is more, an A. tumefaciens mutant carrying large-deletion (~85 kb) in genome was successfully constructed by Cre/loxP system. Here, we described the application of combination of Cre/loxP system and triple recombineering to efficiently excise genomic segment in A. tumefaciens, which also would facilitate efficient construction of multiple gene disruptions in A. tumefaciens.

  12. Osterix-Cre Labeled Progenitor Cells Contribute to the Formation and Maintenance of the Bone Marrow Stroma

    Science.gov (United States)

    Liu, Yaling; Strecker, Sara; Wang, Liping; Kronenberg, Mark S.; Wang, Wen; Rowe, David W.; Maye, Peter

    2013-01-01

    We have carried out fate mapping studies using Osterix-EGFPCre and Osterix-CreERt animal models and found Cre reporter expression in many different cell types that make up the bone marrow stroma. Constitutive fate mapping resulted in the labeling of different cellular components located throughout the bone marrow, whereas temporal fate mapping at E14.5 resulted in the labeling of cells within a region of the bone marrow. The identity of cell types marked by constitutive and temporal fate mapping included osteoblasts, adipocytes, vascular smooth muscle, perineural, and stromal cells. Prolonged tracing of embryonic precursors labeled at E14.5dpc revealed the continued existence of their progeny up to 10 months of age, suggesting that fate mapped, labeled embryonic precursors gave rise to long lived bone marrow progenitor cells. To provide further evidence for the marking of bone marrow progenitors, bone marrow cultures derived from Osterix-EGFPCre/Ai9 mice showed that stromal cells retained Cre reporter expression and yielded a FACS sorted population that was able to differentiate into osteoblasts, adipocytes, and chondrocytes in vitro and into osteoblasts, adipocytes, and perivascular stromal cells after transplantation. Collectively, our studies reveal the developmental process by which Osterix-Cre labeled embryonic progenitors give rise to adult bone marrow progenitors which establish and maintain the bone marrow stroma. PMID:23951132

  13. Electric fields generated by synchronized oscillations of microtubules, centrosomes and chromosomes regulate the dynamics of mitosis and meiosis

    Directory of Open Access Journals (Sweden)

    Zhao Yue

    2012-07-01

    Full Text Available Abstract Super-macromolecular complexes play many important roles in eukaryotic cells. Classical structural biological studies focus on their complicated molecular structures, physical interactions and biochemical modifications. Recent advances concerning intracellular electric fields generated by cell organelles and super-macromolecular complexes shed new light on the mechanisms that govern the dynamics of mitosis and meiosis. In this review we synthesize this knowledge to provide an integrated theoretical model of these cellular events. We suggest that the electric fields generated by synchronized oscillation of microtubules, centrosomes, and chromatin fibers facilitate several events during mitosis and meiosis, including centrosome trafficking, chromosome congression in mitosis and synapsis between homologous chromosomes in meiosis. These intracellular electric fields are generated under energy excitation through the synchronized electric oscillations of the dipolar structures of microtubules, centrosomes and chromosomes, three of the super-macromolecular complexes within an animal cell.

  14. Impaired Ca(2+ signaling in β-cells lacking leptin receptors by Cre-loxP recombination.

    Directory of Open Access Journals (Sweden)

    Eva Tudurí

    Full Text Available Obesity is a major risk factor for diabetes and is typically associated with hyperleptinemia and a state of leptin resistance. The impact of chronically elevated leptin levels on the function of insulin-secreting β-cells has not been elucidated. We previously generated mice lacking leptin signaling in β-cells by using the Cre-loxP strategy and showed that these animals develop increased body weight and adiposity, hyperinsulinemia, impaired glucose-stimulated insulin secretion and insulin resistance. Here, we performed several in vitro studies and observed that β-cells lacking leptin signaling in this model are capable of properly metabolizing glucose, but show impaired intracellular Ca(2+ oscillations and lack of synchrony within the islets in response to glucose, display reduced response to tolbutamide and exhibit morphological abnormalities including increased autophagy. Defects in intracellular Ca(2+ signaling were observed even in neonatal islets, ruling out the possible contribution of obesity to the β-cell irregularities observed in adults. In parallel, we also detected a disrupted intracellular Ca(2+ pattern in response to glucose and tolbutamide in control islets from adult transgenic mice expressing Cre recombinase under the rat insulin promoter, despite these animals being glucose tolerant and secreting normal levels of insulin in response to glucose. This unexpected observation impeded us from discerning the consequences of impaired leptin signaling as opposed to long-term Cre expression in the function of insulin-secreting cells. These findings highlight the need to generate improved Cre-driver mouse models or new tools to induce Cre recombination in β-cells.

  15. La regulación de los servicios de electricidad en Argentina y Brasil (1890-1962 Electric utility regulation in Argentina and Brazil (1890-1962

    Directory of Open Access Journals (Sweden)

    Alexandre Macchione Saes

    2012-08-01

    Full Text Available El artículo analiza la evolución de la regulación del sector eléctrico en Argentina y Brasil entre 1890 y 1960. Desde la instalación de las primeras usinas eléctricas a fines del siglo diecinueve hasta los años treinta, el control de las empresas concesionarias estuvo a cargo de las autoridades municipales en ambos países. No obstante, la similar estructura de los sistemas eléctricos en Argentina y en Brasil, la participación del estado en la regulación de este sector estratégico para el desarrollo económico, se produjo en diferentes coyunturas. Como resultado de la crisis de 1930, el gobierno brasileño transformó los principios jurídicos que reglamentaban la gestión de la electricidad aplicando un criterio de regulación discrecional; mientras que el estado argentino intervino una década más tarde, nacionalizando las empresas. Mediante la comparación de las trayectorias regulatorias en ambos países, se identifican las divergencias en las políticas eléctricas y su impacto en los sistemas eléctricos en los años de la segunda posguerra.This article compares the evolution of electric utility regulation in Argentina and Brazil between 1890 and 1960. From the installation of electrical systems in the 19th century until the 1930s, electrical utility companies were controlled by the local authorities in both countries. The structure of electrical systems was similar in Argentina and Brazil, however the state regulation of electric utilities took place at different times. As a result of the 1930's crisis, the Brazilian government introduced a new legal approach by applying a discretionary regulation. On the other hand, the Argentinean government intervened one decade later, nationalizing the companies. By comparing both regulatory trajectories, the divergences as well as the effects of each policy on the electrical utility systems in the second postward period, are identified.

  16. Transgenic mice exhibiting inducible and spontaneous Cre activities driven by a bovine keratin 5 promoter that can be used for the conditional analysis of basal epithelial cells in multiple organs

    Directory of Open Access Journals (Sweden)

    Liang Chih-Chia

    2009-01-01

    Full Text Available Abstract Background Cre/loxP-mediated genetic modification is the most widely used conditional genetic approach used in the mouse. Engineered Cre and the mutated ligand-binding domain of estrogen receptor fusion recombinase (CreERT allow temporal control of Cre activity. Results In this study, we have generated two distinct transgenic mouse lines expressing CreERT, which show 4-hydroxytamoxifen (4-OHT-inducible and spontaneous (4-OHT-independent Cre activities, referred to Tg(BK5-CreERTI and Tg(BK5-CreERTS, respectively. The transgenic construct is driven by the bovine Keratin 5 promoter, which is active in the basal epithelial lineage of stratified and pseudo-stratified epithelium across multiple organs. Despite the difference in 4-OHT dependency, the Tg(BK5-CreERTI and Tg(BK5-CreERTS mouse lines shared similar Cre-mediated recombination among various organs, except for unique mammary epithelial Cre activity in Tg(BK5-CreERTS females. Conclusion These two new transgenic mouse lines for the analysis of basal epithelial function and for the genetic modification have been created allowing the identification of these cell lineages and analysis of their differentiation during embryogenesis, during perinatal development and in adult mice.

  17. Implementation of an Electric Vehicle Test Bed Controlled by a Virtual Power Plant for Contributing to Regulating Power Reserves

    DEFF Research Database (Denmark)

    Marra, Francesco; Sacchetti, Dario; Pedersen, Anders Bro

    2012-01-01

    and communication interfaces, is able to respond in real-time to smart grid control signals. The EV test bed is equipped with a Lithium-ion battery pack, a Battery Management System (BMS), a charger and a Vehicle-to-Grid (V2G) unit for feeding power back to the grid. The designed solution serves......With the increased focus on Electric Vehicles (EV) research and the potential benefits they bring for smart grid applications, there is a growing need for an evaluation platform connected to the electricity grid. This paper addresses the design of an EV test bed, which using real EV components...

  18. Cyclic AMP induces IPC leukemia cell apoptosis via CRE-and CDK-dependent Bim transcription.

    Science.gov (United States)

    Huseby, S; Gausdal, G; Keen, T J; Kjærland, E; Krakstad, C; Myhren, L; Brønstad, K; Kunick, C; Schwede, F; Genieser, H-G; Kleppe, R; Døskeland, S O

    2011-12-08

    The IPC-81 cell line is derived from the transplantable BNML model of acute myelogenic leukemia (AML), known to be a reliable predictor of the clinical efficiency of antileukemic agents, like the first-line AML anthracycline drug daunorubicin (DNR). We show here that cAMP acted synergistically with DNR to induce IPC cell death. The DNR-induced death differed from that induced by cAMP by (1) not involving Bim induction, (2) being abrogated by GSK3β inhibitors, (3) by being promoted by the HSP90/p23 antagonist geldanamycin and truncated p23 and (4) by being insensitive to the CRE binding protein (CREB) antagonist ICER and to cyclin-dependent protein kinase (CDK) inhibitors. In contrast, the apoptosis induced by cAMP correlated tightly with Bim protein expression. It was abrogated by Bim (BCL2L11) downregulation, whether achieved by the CREB antagonist ICER, by CDK inhibitors, by Bim-directed RNAi, or by protein synthesis inhibitor. The forced expression of BimL killed IPC-81(WT) cells rapidly, Bcl2-overexpressing cells being partially resistant. The pivotal role of CREB and CDK activity for Bim transcription is unprecedented. It is also noteworthy that newly developed cAMP analogs specifically activating PKA isozyme I (PKA-I) were able to induce IPC cell apoptosis. Our findings support the notion that AML cells may possess targetable death pathways not exploited by common anti-cancer agents.

  19. Tracking proliferative history in lymphocyte development with cre-mediated sister chromatid recombination.

    Directory of Open Access Journals (Sweden)

    Baojun Zhang

    2013-10-01

    Full Text Available Tracking and isolating live cells based on their proliferative history in live animals remains a technical challenge in animal studies. We have designed a genetic marking system for tracking the proliferative frequency and history of lymphocytes during their development and homeostatic maintenance. This system is based on activation of a fluorescent marker after Cre-dependent recombination between sister chromatids at a specially designed tandem loxP site, named Tlox. We have demonstrated the utility of the Tlox system in tracking proliferative windows of B and T lymphocyte development. We have further applied the Tlox system in the analysis of the proliferative behavior and homeostatic maintenance of Vγ1.1 positive γδ T cells. Our data show that Vγ1.1 T cells generated in neonatal but not adult life are able to expand in the thymus. The expanded Vγ1.1 T cells are preferentially maintained in the liver but not in lymphoid organs. It has been shown that numbers of Vγ1.1 T cells were dramatically increased in the lymphoid organs of Id3 deficient mice. By combining BrdU and Tlox assays we show that this phenotype is primarily due to enhanced neonatal expansion and subsequent retention of Vγ1.1 T cells. Thus, the Tlox system provides a new genetic tool to track clonal expansion within a defined cell population or tissue type in live animals.

  20. Constructing nanoporous carbon nanotubes/Bi2Te3 composite for synchronous regulation of the electrical and thermal performances

    Science.gov (United States)

    Zhang, Qihao; Xu, Leilei; Zhou, Zhenxing; Wang, Lianjun; Jiang, Wan; Chen, Lidong

    2017-02-01

    Porous nanograined thermoelectric materials exhibit low thermal conductivity due to scattering of phonons by pores, which are favorable for thermoelectric applications. However, the benefit is not large enough to overcome the deficiency in the electrical performance. Herein, an approach is presented to reduce the thermal conductivity and synchronously enhance the electrical conductivity through constructing a nanoporous thermoelectric composite. Carbon nanotubes (CNTs) are truncated and homogeneously dispersed within the Bi2Te3 matrix by a cryogenic grinding (CG) technique for the first time, which efficiently suppress the Bi2Te3 grain growth and create nanopores with the size ranging from dozens to hundreds of nanometers. The lattice thermal conductivity is substantially decreased by broad wavelength phonon scattering resulting from nanopores, increased grain boundaries, and newly formed interfaces. Meanwhile, the electrical conductivity is improved due to the enhanced carrier mobility, which may originate from the bridging effect between the Bi2Te3 grains and CNTs. The maximum ZT is improved by almost a factor of 2 due to the simultaneous optimization of electrical and thermal performances. Our study demonstrates the superiority of constructing a bulk thermoelectric composite with nanopores by the uniform dispersion of CNTs through a CG technique for enhanced thermoelectric properties, which provides a wider approach to thermoelectric nanostructure engineering.

  1. Natural monopolies regulation: the electric power distribution tariffs case; Regulacao dos monopolios naturais: o caso das tarifas de distribuicao de energia eletrica

    Energy Technology Data Exchange (ETDEWEB)

    Guillo, Vicente Andreu; Sauer, Ildo Luis [Sao Paulo Univ., SP (Brazil). Programa Interunidades de Pos-Graduacao em Energia]. E-mail: illsauer@iee.usp.br

    1999-07-01

    The central issue of debate was the need to align the energy sector's options and organization with changing global patterns of economic and social development, characterized by the increasing role played by the private sector, greater integration in the world economy, and new economic and social priorities such as efficiency, decentralization, deregulation, and a closer attention to environmental issues. In this context, the aim of this work is to review the natural monopolies theory, regulation regimes, and the energy distribution tariffs of Brazilian electric power sector.

  2. Electricity and gas market observatory. 4. Quarter 2006; Observatoire des marches de l'electricite et du gaz. 4. trimestre 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    Since July 1, 2004, all electricity and gas consumers can be eligible according to their consumption site, as long as all or part of the electricity or gas consumed is designed for non-residential use. The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). It completes the information already published by CRE: - practical information for eligible customers: consumer guide, list of suppliers, - communications regarding markets running; CRE's annual activity report. Content: A - The electricity market: The retail electricity market (Introduction, Eligible customer segments and their respective weights, Status at January 1, 2007, Dynamic analysis: 4. Quarter 2006); The wholesale electricity market (Introduction, Traded volumes on the French wholesale electricity market and comparison with European markets, Prices on the French wholesale electricity market and European comparison, Import and export volumes, Concentration of the French electricity market, Striking facts of the 4. 2006 quarter); B - The gas market: The retail gas market (Introduction, The eligible customer segments and their respective weights, Status at January 1, 2007); The wholesale gas market (Gas pricing and gas markets in Europe, The wholesale market in France); C - Appendices: Electricity and gas market observatories combined glossary, Specific electricity market observatory glossary, Specific gas market observatory glossary.

  3. Dermal Delivery of Constructs Encoding Cre Recombinase to Induce Skin Tumors in PtenLoxP/LoxP;BrafCA/+ Mice

    Directory of Open Access Journals (Sweden)

    Marcel A. Deken

    2016-12-01

    Full Text Available Current genetically-engineered mouse melanoma models are often based on Tyr::CreERT2-controlled MAPK pathway activation by the BRAFV600E mutation and PI3K pathway activation by loss of PTEN. The major drawback of these models is the occurrence of spontaneous tumors caused by leakiness of the Tyr::CreERT2 system, hampering long-term experiments. To address this problem, we investigated several approaches to optimally provide local delivery of Cre recombinase, including injection of lentiviral particles, DNA tattoo administration and particle-mediated gene transfer, to induce melanomas in PtenLoxP/LoxP;BrafCA/+ mice lacking the Tyr::CreERT2 allele. We found that dermal delivery of the Cre recombinase gene under the control of a non-specific CAG promoter induced the formation of melanomas, but also keratoacanthoma and squamous cell carcinomas. Delivery of Cre recombinase DNA under the control of melanocyte-specific promoters in PtenLoxP/LoxP;BrafCA/+ mice resulted in sole melanoma induction. The growth rate and histological features of the induced tumors were similar to 4-hydroxytamoxifen-induced tumors in Tyr::CreERT2;PtenLoxP/LoxP;BrafCA/+ mice, while the onset of spontaneous tumors was prevented completely. These novel induction methods will allow long-term experiments in mouse models of skin malignancies.

  4. Effect and potential of grid price regulation on the promotion of electrical efficiency; Wirkung und Potenzial der Netzpreisregulierung fuer die Foerderung der Stromeffizienz. Eine Modellbetrachtung - Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Vettori, A.; Iten, R. [Infras, Zuerich (Switzerland); Worm, H.; Vaterlaus, S. [Polynomics AG, Olten (Switzerland); Spielmann, Ch. [IWB, Basel (Switzerland)

    2009-11-15

    This comprehensive report for the Swiss Federal Office of Energy (SFOE) presents the results of a theoretical analysis concerning the question if further development of grid pricing regulations can help increase efficiency in the use of electricity. Two possible stages in the implementation of grid price regulation are examined and discussed. Knowledge gained from theoretical analysis and from experience made in foreign countries is discussed. Various pricing models are examined and their cost-effectiveness discussed. The models and data used and the results obtained from the analysis are presented and discussed. The report is complemented with an appendix with details on selected results, modelling methods and tools used along with a glossary and a literature list.

  5. On the Use of Energy Storage Technologies for Regulation Services in Electric Power Systems with Significant Penetration of Wind Energy

    DEFF Research Database (Denmark)

    Yang, Bo; Makarov, Yuri; Desteese, John

    2008-01-01

    Energy produced by intermittent renewable resources is sharply increasing in the United States. At high penetration levels, volatility of wind power production could cause additional problems for the power system balancing functions such as regulation. This paper reports some partial results...... of a project work, recently conducted by the Pacific Northwest National Laboratory (PNNL) for Bonneville Power Administration (BPA). The project proposes to mitigate additional intermittency with the help of Wide Area Energy Management System (WAEMS) that would provide a two-way simultaneous regulation service...... for the BPA and California ISO systems by using a large energy storage facility. The paper evaluates several utility-scale energy storage technology options for their usage as regulation resources. The regulation service requires a participating resource to quickly vary its power output following the rapidly...

  6. Electricity and gas market observatory. 4. quarter 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    Since July 1, 2004, all electricity and gas consumers can be eligible according to their consumption site, as long as all or part of the electricity or gas consumed is designed for non-residential use. The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web-site (www.cre.fr). It presents: The electricity market; The retail electricity market: Eligible customer segments and their respective weights, Status at January 1, 2007, Dynamic analysis: 4. Quarter 2007; The wholesale electricity market: Traded volumes on the French wholesale electricity market and comparison with European markets, Prices on the French wholesale electricity market and European comparison, Import and export volumes, Concentration of the French electricity market, Striking facts of the 4. 2006 quarter; The gas market; The retail gas market: The eligible customer segments and their respective weights, Status at January 1. 2007; The wholesale gas market: Gas pricing and gas markets in Europe,The wholesale market in France. Some glossaries are attached to the document: Electricity and gas market observatories combined glossary; Specific electricity market observatory glossary; Specific gas market observatory glossary.

  7. Electricity and gas market observatory. 1. quarter 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    Since July 1, 2004, all electricity and gas consumers can be eligible according to their consumption site, as long as all or part of the electricity or gas consumed is designed for non-residential use. The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web-site (www.cre.fr). It presents: The electricity market; The retail electricity market: Eligible customer segments and their respective weights, Status at April 1, 2007, Dynamic analysis: 1. Quarter 2007; The wholesale electricity market: Traded volumes on the French wholesale electricity market and comparison with European markets, Prices on the French wholesale electricity market and European comparison, Import and export volumes, Concentration of the French electricity market, Striking facts of the 1. 2007 quarter; The gas market; The retail gas market: The eligible customer segments and their respective weights, Status at April 1. 2007; The wholesale gas market: Gas pricing and gas markets in Europe,The wholesale market in France. Some glossaries are attached to the document: Electricity and gas market observatories combined glossary; Specific electricity market observatory glossary; Specific gas market observatory glossary.

  8. Interleukin-6 deletion in mice driven by aP2-Cre-ERT2 prevents against high-fat diet-induced gain weight and adiposity in female mice.

    Science.gov (United States)

    Navia, B; Ferrer, B; Giralt, M; Comes, G; Carrasco, J; Molinero, A; Quintana, A; Leclerc, J; Viollet, B; Señarís, R M; Hidalgo, J

    2014-08-01

    Interleukin-6 (IL-6) is a major cytokine controlling body weight and metabolism, but because many types of cells can synthesize and respond to IL-6 considerable uncertainty still exists about the mechanisms underlying IL-6 effects. Therefore, the aim of this study was to analyse the effects of tissue-specific deletion of IL-6 using a fatty acid binding protein (aP2) promoter-Cre inducible system (aP2-Cre-ERT2). Tissue-specific IL-6 KO mice (aP2-IL-6 KO mice) were produced upon tamoxifen administration and were fed a high-fat diet (HFD, 58.4% kcal from fat) or a control diet (18%) for 14 weeks. aP2-IL-6 KO female mice on a HFD gained less weight and adiposity than littermate wild-type mice, but these effects were not observed in males. Hypothalamic factors such as NPY and AgRP showed a pattern of expression consistent with this sex-specific phenotype. PGC-1α expression was increased in several tissues in aP2-IL-6 KO female mice, which is compatible with increased energy expenditure. Serum leptin, insulin, glucose, cholesterol and triglycerides levels were increased by HFD, and in females IL-6 deficiency reversed this effect in the case of insulin and cholesterol. HFD induced impaired responses to insulin and glucose tolerance tests, but no significant differences between genotypes were observed. The present results demonstrate that deletion of IL-6 driven by aP2-Cre regulates body weight, body fat and metabolism in a sex-specific fashion. © 2014 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.

  9. Dynamics of risk management in the regulated contracting environment of the Brazilian electrical sector; Dinamica da gestao de riscos no ambiente de contratacao regulada do setor eletrico brasileiro

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Fabio Cavaliere de

    2008-11-15

    This thesis analyses the dynamics of risk management in the regulated contracting environment of the Brazilian electrical sector. As in the new model the distribution utilities are obliged to ensure energy contracting to supply their whole markets, these companies became exposed to volume risks related to the demand uncertainties. So the new model following similar directives from the first phase developed management tools to mitigate the new risks mentioned above. As in a well structured market it is expected that the risks should be evenly shared or that risk management tools should be provided for all, this work intended to analyze the dynamics of the designed mechanisms and their impacts over the regulated market. In this work the risks of the electricity markets are studied and the management tools devised for the Brazilian market are identified. Finally for understanding the dynamics a thorough analysis of the auctions, of the mechanism for compensation of surplus and deficits and tariffs are carried on. The results show that the management tools were regularly employed by distribution companies, but the risks were merely transferred to the captive customers leading to distortions and asymmetries that conflict with the basis that guided the second phase of the reform. The work proposes remedial actions to mitigate the observed impacts and to provide risk management tools to the captive customer. (author)

  10. Towards a future with large penetration of distributed generation: Is the current regulation of electricity distribution ready? Regulatory recommendations under a European perspective

    Energy Technology Data Exchange (ETDEWEB)

    Cossent, Rafael [Instituto de Investigacion Tecnologica, Escuela Tecnica Superior de Ingenieria, Universidad Pontificia Comillas, C/ Quintana 21, 28008 Madrid (Spain)], E-mail: Rafael.Cossent@iit.upcomillas.es; Gomez, Tomas; Frias, Pablo [Instituto de Investigacion Tecnologica, Escuela Tecnica Superior de Ingenieria, Universidad Pontificia Comillas, C/ Quintana 21, 28008 Madrid (Spain)

    2009-03-15

    The European Energy Policy promotes renewable energy sources and energy efficiency as means to mitigate environmental impact, increase security of supply and ensure economic competitiveness. As a result, the penetration levels of distributed generation (DG) in electricity networks are bound to increase. Distribution networks and distribution system operators (DSOs) will be especially affected by growing levels of DG. This paper reviews the current regulation of distribution in the European Union Member States, focusing on those aspects that might hinder the future integration of DG. Several regulatory issues that may hinder a successful integration of DG have been identified. Recommendations to improve the current situation are proposed. Regarding economic signals sent to DG, connection charges and cost-reflective use-of-system charges together with incentives to provide ancillary services are the key aspects. Concerning DSOs regulation, unbundling from generation and supply according to the European Electricity Directive, incentives for optimal planning and network operation considering DG, including energy losses and quality of service, and innovation schemes to migrate to active networks are the most relevant topics.

  11. Towards a future with large penetration of distributed generation. Is the current regulation of electricity distribution ready? Regulatory recommendations under a European perspective

    Energy Technology Data Exchange (ETDEWEB)

    Cossent, Rafael; Gomez, Tomas; Frias, Pablo [Instituto de Investigacion Tecnologica, Escuela Tecnica Superior de Ingenieria, Universidad Pontificia Comillas, C/ Quintana 21, 28008 Madrid (Spain)

    2009-03-15

    The European Energy Policy promotes renewable energy sources and energy efficiency as means to mitigate environmental impact, increase security of supply and ensure economic competitiveness. As a result, the penetration levels of distributed generation (DG) in electricity networks are bound to increase. Distribution networks and distribution system operators (DSOs) will be especially affected by growing levels of DG. This paper reviews the current regulation of distribution in the European Union Member States, focusing on those aspects that might hinder the future integration of DG. Several regulatory issues that may hinder a successful integration of DG have been identified. Recommendations to improve the current situation are proposed. Regarding economic signals sent to DG, connection charges and cost-reflective use-of-system charges together with incentives to provide ancillary services are the key aspects. Concerning DSOs regulation, unbundling from generation and supply according to the European Electricity Directive, incentives for optimal planning and network operation considering DG, including energy losses and quality of service, and innovation schemes to migrate to active networks are the most relevant topics. (author)

  12. Establishment of a Cre/loxP recombination system for N-terminal epitope tagging of genes in Tetrahymena

    Directory of Open Access Journals (Sweden)

    Mochizuki Kazufumi

    2010-07-01

    Full Text Available Abstract Background Epitope tagging is a powerful strategy to study the function of proteins. Although tools for C-terminal protein tagging in the ciliated protozoan Tetrahymena thermophila have been developed, N-terminal protein tagging in this organism is still technically demanding. Results In this study, we have established a Cre/loxP recombination system in Tetrahymena and have applied this system for the N-terminal epitope tagging of Tetrahymena genes. Cre can be expressed in Tetrahymena and localizes to the macronucleus where it induces precise recombination at two loxP sequences in direct orientation in the Tetrahymena macronuclear chromosome. This Cre/loxP recombination can be used to remove a loxP-flanked drug-resistance marker from an N-terminal tagging construct after it is integrated into the macronucleus. Conclusions The system established in this study allows us to express an N-terminal epitope tagged gene from its own endogenous promoter in Tetrahymena.

  13. The political economy of (de)regulation: Theory and evidence from the U.S. electricity market

    NARCIS (Netherlands)

    Guerriero, C.

    2010-01-01

    The decision to introduce competition into regulated industries is a key issue in economics. Provided that the demand is sufficiently inelastic, competition assures lower allocative distortions at the cost of weaker cost-reducing investment incentives via lower profits. Hence, deregulation is more

  14. Graphene/phase change material nanocomposites: light-driven, reversible electrical resistivity regulation via form-stable phase transitions.

    Science.gov (United States)

    Wang, Yunming; Mi, Hongyi; Zheng, Qifeng; Ma, Zhenqiang; Gong, Shaoqin

    2015-02-04

    Innovative photoresponsive materials are needed to address the complexity of optical control systems. Here, we report a new type of photoresponsive nanomaterial composed of graphene and a form-stable phase change material (PCM) that exhibited a 3 orders of magnitude change in electrical resistivity upon light illumination while retaining its overall original solid form at the macroscopic level. This dramatic change in electrical resistivity also occurred reversibly through the on/off control of light illumination. This was attributed to the reversible phase transition (i.e., melting/recrystallization) behavior of the microscopic crystalline domains present in the form-stable PCM. The reversible phase transition observed in the graphene/PCM nanocomposite was induced by a reversible temperature change through the on/off control of light illumination because graphene can effectively absorb light energy and convert it to thermal energy. In addition, this graphene/PCM nanocomposite also possessed excellent mechanical properties. Such photoresponsive materials have many potential applications, including flexible electronics.

  15. Insights into the preferential order of strand exchange in the Cre/loxP recombinase system: impact of the DNA spacer flanking sequence and flexibility

    Science.gov (United States)

    Abi-Ghanem, Josephine; Samsonov, Sergey A.; Pisabarro, M. Teresa

    2015-03-01

    The Cre/loxP system is widely used as a genetic tool to manipulate DNA. Cre recombinase catalyzes site-specific recombination between 34 bp loxP sites. Each loxP site is recognized by two Cre molecules assuming a cleaving (CreC) and non-cleaving (CreNC) activity. Despite the symmetry in the sequences of the arms of loxP, available biochemical data show strong evidence that the recombination reaction is asymmetric with a preferred strand exchange order. The asymmetry comes from the spacer separating the two sets of palindromic arms of the loxP sequence. However, it remains to be understood how this preferential order is established. We apply computational structure-based methods and perform a thorough detailed analysis of available structural and biochemical information on the Cre/loxP system in order to investigate such asymmetry in the recombination, and we propose a rationale to explain the determinants favoring the strand exchange order. We show that the structural properties of the DNA flanking sequence of the spacer guide the recombination, and we establish the role of residues R118, R121 and K122 from CreC, which contact the spacer region and by clamping the DNA inhibit the cleavage on the second arm of loxP. Our studies give an atomistic insight on the synapsis state of the recombination process in the Cre/loxP system and highlight the importance of the flexibility and other intrinsic properties of the flanking regions of the DNA spacer to establish a preferential strand exchange order.

  16. Transcutaneous electrical nerve stimulation on Yongquan acupoint reduces CFA-induced thermal hyperalgesia of rats via down-regulation of ERK2 phosphorylation and c-Fos expression.

    Science.gov (United States)

    Yang, Lin; Yang, Lianxue; Gao, Xiulai

    2010-07-01

    Activation of extracellular signal-regulated kinase-1/2 (ERK1/2) and its involvement in regulating gene expression in spinal dorsal horn, cortical and subcortical neurons by peripheral noxious stimulation contribute to pain hypersensitivity. Transcutaneous electrical nerve stimulation (TENS) is a treatment used in physiotherapy practice to promote analgesia in acute and chronic inflammatory conditions. In this study, a total number of 114 rats were used for three experiments. Effects of complete Freund's adjuvant (CFA)-induced inflammatory pain hypersensitivity and TENS analgesia on ERK1/2 phosphorylation and c-Fos protein expression were examined by using behavioral test, Western blot, and immunostaining methods. We found that CFA injection caused an area of localized swelling, erythema, hypersensitivity to thermal stimuli, the decreased response time of hind paw licking (HPL), as well as upregulation of c-Fos protein expression and ERK2 phosphorylation in the ipsilateral spinal dorsal horn and the contralateral primary somatosensory area of cortex and the amygdala of rats. TENS on Yongquan acupoint for 20 min produced obvious analgesic effects as demonstrated with increased HPL to thermal stimuli of CFA-treated rats. In addition, TENS application suppressed the CFA-induced ERK2 activation and c-Fos protein expression. These results suggest that down-regulation of ERK2 phosphorylation and c-Fos expression were involved in TENS inhibition on CFA-induced thermal hyperalgesia of rats.

  17. Nestin-Cre mice are affected by hypopituitarism, which is not due to significant activity of the transgene in the pituitary gland.

    Directory of Open Access Journals (Sweden)

    Christophe Galichet

    2010-07-01

    Full Text Available Nestin-Cre mice express Cre recombinase under control of the rat nestin promoter and central nervous system (CNS enhancer. While endogenous Nestin is expressed in some other tissues including the pituitary gland, Nestin-Cre mice induce recombination predominantly in the CNS. For this reason, they have been widely used to explore gene function or cell fate in the latter. Pituitary hormonal deficiencies, or hypopituitarism, are associated with a wide range of symptoms and with a significant morbidity. These can have a neural and/or a pituitary origin as the gland's secretions are controlled by the hypothalamus. We report here that Nestin-Cre mice themselves are affected by mild hypopituitarism. Hence, physiological consequences are expected, especially in combination with defects resulting from Cre mediated deletion of any gene under investigation. To further investigate the origin of this phenotype, we re-examined the activity of the transgene. We compared it with expression of Nestin itself in the context of the hypothalamo-pituitary axis, especially in the light of a recent report showing pituitary Nestin-Cre activity, which contrasts with previous data. Our results disagree with those of this recent study and do not support the claim that Nestin positive cells are present in the pituitary anlagen, the Rathke's pouch (RP. Moreover we did not observe any significant activity in the post-natal pituitary, in agreement with the initial report.

  18. Nestin-Cre Mice Are Affected by Hypopituitarism, Which Is Not Due to Significant Activity of the Transgene in the Pituitary Gland

    Science.gov (United States)

    Galichet, Christophe; Lovell-Badge, Robin; Rizzoti, Karine

    2010-01-01

    Nestin-Cre mice express Cre recombinase under control of the rat nestin promoter and central nervous system (CNS) enhancer. While endogenous Nestin is expressed in some other tissues including the pituitary gland, Nestin-Cre mice induce recombination predominantly in the CNS. For this reason, they have been widely used to explore gene function or cell fate in the latter. Pituitary hormonal deficiencies, or hypopituitarism, are associated with a wide range of symptoms and with a significant morbidity. These can have a neural and/or a pituitary origin as the gland's secretions are controlled by the hypothalamus. We report here that Nestin-Cre mice themselves are affected by mild hypopituitarism. Hence, physiological consequences are expected, especially in combination with defects resulting from Cre mediated deletion of any gene under investigation. To further investigate the origin of this phenotype, we re-examined the activity of the transgene. We compared it with expression of Nestin itself in the context of the hypothalamo-pituitary axis, especially in the light of a recent report showing pituitary Nestin-Cre activity, which contrasts with previous data. Our results disagree with those of this recent study and do not support the claim that Nestin positive cells are present in the pituitary anlagen, the Rathke's pouch (RP). Moreover we did not observe any significant activity in the post-natal pituitary, in agreement with the initial report. PMID:20625432

  19. Generation of a tenascin-C-CreER2 knockin mouse line for conditional DNA recombination in renal medullary interstitial cells.

    Directory of Open Access Journals (Sweden)

    Wenjuan He

    Full Text Available Renal medullary interstitial cells (RMIC are specialized fibroblast-like cells that exert important functions in maintaining body fluid homeostasis and systemic blood pressure. Here, we generated a RMIC specific tenascin-C promoter driven inducible CreER2 knockin mouse line with an EGFP reporter. Similar as endogenous tenascin-C expression, the reporter EGFP expression in the tenascin-C-CreER2(+/- mice was observed in the inner medulla of the kidney, and co-localized with COX2 but not with AQP2 or AQP1, suggesting selective expression in RMICs. After recombination (tenascin-C-CreER2(+/-/ROSA26-lacZ(+/- mice + tamoxifen, β-gal activity was restricted to the cells in the inner medulla of the kidney, and didn't co-localize with AQP2, consistent with selective Cre recombinase activity in RMICs. Cre activity was not obvious in other major organs or without tamoxifen treatment. This inducible RMIC specific Cre mouse line should therefore provide a novel tool to manipulate genes of interest in RMICs.

  20. Elevated cJUN expression and an ATF/CRE site within the ATF3 promoter contribute to activation of ATF3 transcription by the amino acid response.

    Science.gov (United States)

    Fu, Lingchen; Kilberg, Michael S

    2013-02-15

    Mammalian cells respond to amino acid deprivation through multiple signaling pathways referred to as the amino acid response (AAR). Transcription factors mediate the AAR after their activation by several mechanisms; examples include translational control (activating transcription factor 4, ATF4), phosphorylation (p-cJUN), and transcriptional control (ATF3). ATF4 induces ATF3 transcription through a promoter-localized C/EBP-ATF response element (CARE). The present report characterizes an ATF/CRE site upstream of the CARE that also contributes to AAR-induced ATF3 transcription. ATF4 binds to the ATF/CRE and CARE sequences and both are required for a maximal response to ATF4 induction. ATF3, which antagonizes ATF4 and represses its own gene, also exhibited binding activity to the ATF/CRE and CARE sequences. The AAR resulted in elevated total cJUN and p-cJUN protein levels and both forms exhibited binding activity to the ATF/CRE and CARE ATF3 sequences. Knockdown of AAR-enhanced cJUN expression blocked induction of the ATF3 gene and mutation of either the ATF/CRE or the CARE site prevented the cJUN-dependent increase in ATF3-driven luciferase activity. The results indicate that both increased cJUN and the cis-acting ATF/CRE sequence within the ATF3 promoter contribute to the transcriptional activation of the gene during the AAR.

  1. Electric power transmission pricing regulations and efficiency; Il prezzo della trasmissione elettrica: condizioni efficienti e casi concreti

    Energy Technology Data Exchange (ETDEWEB)

    Goldoni, G. [Verona Univ. (Italy)

    1999-03-01

    An efficient-price mechanism for electricity transmission is very hard to find, essentially because of the natural monopoly condition of the grid and its peculiar interactions with generation. The use of Optimal Power Flow Models is difficult to implement and could be easily distorted by strategical behaviour of generators. These models, however, could became a valuable efficiency-test for actual transmission charges and codes. [Italiano] Il prezzo efficiente della trasmissione di energia elettrica e` molto difficile da determinare, sia per le condizioni di monopolio naturale, sia per le strette interazioni con la generazione. I modelli di ottimizzazione che cercano di rappresentare queste interazioni, d`altra parte, non sono semplici da implementare e potrebbero indurre comportamenti strategici, ma possono diventare un utile strumento per giudicare le effettive modalita` tecniche ed economiche di accesso alla rete di trasmissione.

  2. REGULATION OF ELECTRICAL AND RHEOLOGICAL PROPERTIES OF HETEROGENEOUS SYSTEMS ON THE BASIS OF QUARTZ SAND AND GRAPHITE BY MECHANICAL ACTIVATION OF QUARTZ SAND

    Directory of Open Access Journals (Sweden)

    Lopanov Aleksandr Nikolaevich

    2016-08-01

    Full Text Available The authors present the experimental performance of the heat of wetting depending on the number of functional groups on the surface of quartz sand. It is established that in the process of increase of specific surface of fine ground quartz filler naturally there is an increase in the total number of active centers, but their number does not change per unit of surface. The calorimetric method of analysis established that the mechanical activation increases the reactivity centers. This is the result of chemical reactions occurring on the surface, for example, under the influence of water present in the air. The authors observed hydration of surface groups and change in their reactivity. A method of regulation of rheological characteristics of pastes with plasticizing additives in the system ”cement - quartz sand - graphite“ was developed, which was aimed to reduce the ultimate shear stress up to 0.5...1.3 PA and water-composite relationship of a sand mix by 15...25 % while maintaining the viscosity of the paste that provides a uniform application of the composite in screed floors technology possessing electrical conductivity. Polycarboxylate is an efficient plasticizing additive which maintains self-levelling effect of the mixture at optimum water-cement ratio. It was determined that the threshold concentration of electrical current flow in the studied system is 0.15 (wt., at which the aggregation of contacting graphite particles with the formation of a continuous three-dimensional mesh a conducting current is observed in the structure of the composite. The change in the concentration of the graphite allows ensuring the stability of the electrophysical parameters of the resistive materials and structures based on them. In order to increase the electrical conductivity of graphite dispersions in a model system a plasticizer was added, which reduces water-composite ratio in the formation of pastes. Chemical-based plasticizer has no significant

  3. A Bi-Level Optimization Approach to Charging Load Regulation of Electric Vehicle Fast Charging Stations Based on a Battery Energy Storage System

    Directory of Open Access Journals (Sweden)

    Yan Bao

    2018-01-01

    Full Text Available Fast charging stations enable the high-powered rapid recharging of electric vehicles. However, these stations also face challenges due to power fluctuations, high peak loads, and low load factors, affecting the reliable and economic operation of charging stations and distribution networks. This paper introduces a battery energy storage system (BESS for charging load control, which is a more user-friendly approach and is more robust to perturbations. With the goals of peak-shaving, total electricity cost reduction, and minimization of variation in the state-of-charge (SOC range, a BESS-based bi-level optimization strategy for the charging load regulation of fast charging stations is proposed in this paper. At the first level, a day-ahead optimization strategy generates the optimal planned load curve and the deviation band to be used as a reference for ensuring multiple control objectives through linear programming, and even for avoiding control failure caused by insufficient BESS energy. Based on this day-ahead optimal plan, at a second level, real-time rolling optimization converts the control process to a multistage decision-making problem. The predictive control-based real-time rolling optimization strategy in the proposed model was used to achieve the above control objectives and maintain battery life. Finally, through a horizontal comparison of two control approaches in each case study, and a longitudinal comparison of the control robustness against different degrees of load disturbances in three cases, the results indicated that the proposed control strategy was able to significantly improve the charging load characteristics, even with large disturbances. Meanwhile, the proposed approach ensures the least amount of variation in the range of battery SOC and reduces the total electricity cost, which will be of a considerable benefit to station operators.

  4. Solvent Role in the Formation of Electric Double Layers with Surface Charge Regulation: A Bystander or a Key Participant?

    Science.gov (United States)

    Fleharty, Mark E.; van Swol, Frank; Petsev, Dimiter N.

    2016-01-01

    The charge formation at interfaces involving electrolyte solutions is due to the chemical equilibrium between the surface reactive groups and the potential determining ions in the solution (i.e., charge regulation). In this Letter we report our findings that this equilibrium is strongly coupled to the precise molecular structure of the solution near the charged interface. The neutral solvent molecules dominate this structure due to their overwhelmingly large number. Treating the solvent as a structureless continuum leads to a fundamentally inadequate physical picture of charged interfaces. We show that a proper account of the solvent effect leads to an unexpected and complex system behavior that is affected by the molecular and ionic excluded volumes and van der Waals interactions.

  5. Charging operation with high energy efficiency for electric vehicle valve-regulated lead-acid battery system

    Energy Technology Data Exchange (ETDEWEB)

    Ikeya, Tomohiko; Mita, Yuichi; Ishihara, Kaoru [Central Research Inst. of Electric Power Industry (CRIEPI), Komae Res. Lab., Lithium Battery Project, Tokyo (Japan); Sawada, Nobuyuki [Hokkaido Electric Power Co., Sapporo (Japan); Takagi, Sakae; Murakami, Jun-ichi [Tohoku Electric Power Co. Inc., Sendai (Japan); Kobayashi, Kazuyuki [Tokyo Electric Power Co., Yokohama (Japan); Sakabe, Tetsuya [Chubu Electric Power Co., Nagoya (Japan); Kousaka, Eiichi [Hokuriku Electric Power Co., Toyama (Japan); Yoshioka, Haruki [The Kansai Electric Power Co., Osaka (Japan); Kato, Satoru [The Chugoku Electric Power Co., Hiroshima (Japan); Yamashita, Masanori [Shikoku Research Inst. Inc., Takamatsu (Japan); Narisoko, Hayato [The Okinawa Electric Power Co., Naha (Japan); Nishiyama, Kazuo [The Central Electric Power Council, Tokyo (Japan); Adachi, Kazuyuki [Kyushu Electric Power Co., Fukuoka (Japan)

    2000-12-01

    A new, high-energy-efficiency charging operation with as little amount of overcharge as possible is proposed to improve the energy efficiency and the cycle life for an EV valve-regulated lead-acid battery. Under this operation, the EV battery system is charged with 105% of amount of the preceding discharge five out of six times and once with 115% in order that it is fully charged. The cycle lives were estimated using a valve-regulated lead-acid battery system of 12 modules connected in series, by SFUDS79 pattern discharging and measurement of the amount of discharge every 50 cycles. Three-step constant current charging with 115% of amount of the preceding discharge required more than 5 h with the final charging step of more than 210 min, with coulomb efficiency of only 87% and energy efficiency of 74%. On the other hand, under the high-energy-efficiency charging operation, three-step charging with 105% shortens the final charging time to 132 min. It was completed in less than 4 h with coulomb and energy efficiency of 95% and 84%, respectively. This operation increased the energy efficiency from 74% to 83% on average in six charging, and extended the cycle life by about 30% to more than 400 cycles. Decreasing the amount of charge by as much as possible suppressed the corrosion of the grids in the positive plate and the heat evolution in batteries due to shortening of the final charging step. Although the high-energy-efficiency charging operation led to the accumulation of inactive PbSO{sub 4} at the upper part of the negative plate, possibly due to the decreasing amount of overcharge, this operation could prolong the cycle life. Full charging once every six times is though to be effective in suppressing degradation caused by the accumulation of inactive PbSO{sub 4} in the negative plate due to the shortage of charge. (orig.)

  6. Extracellular Cl-regulates electrical slow waves and setting of smooth muscle membrane potential by interstitial cells of Cajal in mouse jejunum.

    Science.gov (United States)

    Saravanaperumal, Siva Arumugam; Gibbons, Simon J; Malysz, John; Sha, Lei; Linden, David R; Szurszewski, Joseph H; Farrugia, Gianrico

    2018-01-01

    What is the central question of this study? The aim was to investigate the roles of extracellular chloride in electrical slow waves and resting membrane potential of mouse jejunal smooth muscle by replacing chloride with the impermeant anions gluconate and isethionate. What is the main finding and its importance? The main finding was that in smooth muscle cells, the resting Cl - conductance is low, whereas transmembrane Cl - movement in interstitial cells of Cajal (ICCs) is a major contributor to the shape of electrical slow waves. Furthermore, the data confirm that ICCs set the smooth muscle membrane potential and that altering Cl - homeostasis in ICCs can alter the smooth muscle membrane potential. Intracellular Cl - homeostasis is regulated by anion-permeable channels and transporters and contributes to excitability of many cell types, including smooth muscle and interstitial cells of Cajal (ICCs). Our aims were to investigate the effects on electrical activity in mouse jejunal muscle strips of replacing extracellular Cl - (Cl - o ) with the impermeant anions gluconate and isethionate. On reducing Cl - o , effects were observed on electrical slow waves, with small effects on smooth muscle membrane voltage (E m ). Restoration of Cl - hyperpolarized smooth muscle E m proportional to the change in Cl - o concentration. Replacement of 90% of Cl - o with gluconate reversibly abolished slow waves in five of nine preparations. Slow waves were maintained in isethionate. Gluconate and isethionate substitution had similar concentration-dependent effects on peak amplitude, frequency, width at half peak amplitude, rise time and decay time of residual slow waves. Gluconate reduced free ionized Ca 2+ in Krebs solutions to 0.13 mm. In Krebs solutions containing normal Cl - and 0.13 mm free Ca 2+ , slow wave frequency was lower, width at half peak amplitude was smaller, and decay time was faster. The transient hyperpolarization following restoration of Cl - o was not observed

  7. High burden of Carbapenem-resistant Enterobacteriaceae (CRE fecal carriage at a teaching hospital: cost-effectiveness of screening in low-resource setting

    Directory of Open Access Journals (Sweden)

    Abdul Rahman Zaidah

    2017-05-01

    Full Text Available Abstract Background Infections by multidrug-resistant gram-negative bacteria (MDR-GNB have been continuously growing and pose challenge to health institution globally. Carbapenem-resistant Enterobacteriacea (CRE was identified as one of the MDR-GNB which has limited treatment options and higher mortality compared to those of sensitive strains. We report an increased burden of CRE fecal carriage at a hospital in the North-eastern region of Malaysia. Methods A retrospective descriptive study from August 2013 to December 2015 was conducted in the Medical Microbiology & Parasitology laboratory of Hospital Universiti Sains Malaysia, which is a tertiary teaching hospital with more than 700 beds. This hospital treats patients with various medical and surgical conditions. Suspected CRE from any clinical specimens received by the laboratory was identified and confirmed using standard protocols. Polymerase chain reaction (PCR assay was performed to determine the genotype. Results Altogether, 8306 Enterobacteriaceae was isolated from various clinical specimens during the study period and 477/8306 (5.74% were CRE. Majority of the isolated CRE were Klebsiella [408/477, (85.5%], of which Klebsiella pneumoniae was the predominant species, 388/408 (95%. CRE were mainly isolated from rectal swab (screening, 235/477 (49.3%; urine, 76/477 (15.9%; blood, 46/477 (9.6% and about 7.1% from tracheal aspirate. One hundred and thirty-six isolates were subjected to genotype determination and., 112/136 (82.4% showed positive detection of New Delhi metallo-β-lactamase 1 (NDM-1 gene (bla NDM1. Conclusion The study noted a high numbers of CRE isolated especially from rectal swabs. Active screening results in significant cost pressures and therefore should be revisited and revised, especially in low resource settings.

  8. Regulation in the electric power industry. A practical manual. 2. ed.; Regulierung in der Energiewirtschaft. Ein Praxishandbuch

    Energy Technology Data Exchange (ETDEWEB)

    Salje, Peter [Hannover Univ. (Germany); Schmidt-Preuss, Matthias (ed.) [Bonn Univ. (Germany); Baur, Juergen F.

    2016-04-01

    The energy transition is taking place with dynamism and breakneck pace. The legislation and process of regulations, European legislation and the practice of FNA and courts form a regulatory framework, which means growing challenges for all players in the energy sector. the cutting-edge issues and developments of practice and theory discussed Edition - After the encouraging uptake of the 1st edition of the best practice manual ''regulation in the energy sector'' the current questions and developments for practice and theory are dealed in a new - second edition. The specifics of the work to be maintained: On the one hand the interdisciplinary - regulatory economic as well as economic - anchoring and on the other hand taking into account the European requirements which are becoming more and more important. Against this background, in particular, the problems raised by the energy transition issues of excellent writers are explained. Here numerous additional topics are included in the manual, such as the investment stimulatory instruments (as the decommissioning ban). Moreover, in the last part, the energy of civil law has been newly added to its width. The same applies to the increasingly important topic of metrology. Other highly topical - in the new edition treated first - Topics include among others the easement agreements and the 20-year time limit and re-allocation of concession contracts, customer equipment, and closed distribution systems. As part of the energy transition the EEG 2014 with conceptual new approaches is a reason for a significant expansion of the relevant chapter. Finally is pointed to the entirely new network planning - from network development plan over the federal requirements planning to final project approval -, which will now be discussed in depth. Authors: Proven experts from science and practice Audience: courts, public authorities and institutions in the energy sector, energy businesses, advocacy and science. [German

  9. Detection of epithelial to mesenchymal transition in airways of a bleomycin induced pulmonary fibrosis model derived from an α-smooth muscle actin-Cre transgenic mouse

    Directory of Open Access Journals (Sweden)

    Yang Xiao

    2007-01-01

    Full Text Available Abstract Background Epithelial to mesenchymal transition (EMT in alveolar epithelial cells (AECs has been widely observed in patients suffering interstitial pulmonary fibrosis. In vitro studies have also demonstrated that AECs could convert into myofibroblasts following exposure to TGF-β1. In this study, we examined whether EMT occurs in bleomycin (BLM induced pulmonary fibrosis, and the involvement of bronchial epithelial cells (BECs in the EMT. Using an α-smooth muscle actin-Cre transgenic mouse (α-SMA-Cre/R26R strain, we labelled myofibroblasts in vivo. We also performed a phenotypic analysis of human BEC lines during TGF-β1 stimulation in vitro. Methods We generated the α-SMA-Cre mouse strain by pronuclear microinjection with a Cre recombinase cDNA driven by the mouse α-smooth muscle actin (α-SMA promoter. α-SMA-Cre mice were crossed with the Cre-dependent LacZ expressing strain R26R to produce the double transgenic strain α-SMA-Cre/R26R. β-galactosidase (βgal staining, α-SMA and smooth muscle myosin heavy chains immunostaining were carried out simultaneously to confirm the specificity of expression of the transgenic reporter within smooth muscle cells (SMCs under physiological conditions. BLM-induced peribronchial fibrosis in α-SMA-Cre/R26R mice was examined by pulmonary βgal staining and α-SMA immunofluorescence staining. To confirm in vivo observations of BECs undergoing EMT, we stimulated human BEC line 16HBE with TGF-β1 and examined the localization of the myofibroblast markers α-SMA and F-actin, and the epithelial marker E-cadherin by immunofluorescence. Results βgal staining in organs of healthy α-SMA-Cre/R26R mice corresponded with the distribution of SMCs, as confirmed by α-SMA and SM-MHC immunostaining. BLM-treated mice showed significantly enhanced βgal staining in subepithelial areas in bronchi, terminal bronchioles and walls of pulmonary vessels. Some AECs in certain peribronchial areas or even a small

  10. Competition compliant wholesale electricity prices. An examination of the regulation on the integrity and transparency of wholesale energy market; Wettbewerbskonforme Stromgrosshandelspreise. Eine Untersuchung der Verordnung ueber die Integritaet und Transparenz des Energiegrosshandelsmarkts

    Energy Technology Data Exchange (ETDEWEB)

    Konar, Selma

    2015-07-01

    The development of wholesale electricity prices showed in recent years a very fluctuating course. The starting point for ensuring competitive compliant electricity prices have uniform rules that establish effective competition in the overall wholesale electricity, ensure greater transparency in the market and prohibit market abuse influence exercised on the wholesale price. The REMIT regulation creates a first union-law rules to this standardized specifications. The volume first examines the transparency, competitiveness, and supervisory structures in the wholesale electricity before legislating a regulation. It is clear, as the transparency and supervisory structures should be designed from the wholesale electricity ideally. On this basis, the work is dealing with the REMIT regulation. The author works out to market participants relevant notification and publication requirements, the follow-up demands on the company as well as the now existing prohibitions on market abuse and the related penalty catalog and analyze the supervisory structures newly created in the wholesale electricity. Here, the work also identified the weaknesses of the regulation and shows suitable solution approaches. [German] Die Entwicklung der Stromgrosshandelspreise zeigte in den letzten Jahren einen sehr schwankenden Verlauf. Ausgangspunkt fuer die Gewaehrleistung wettbewerbskonformer Strompreise sind einheitliche Bestimmungen, die im gesamten Stromgrosshandel einen funktionierenden Wettbewerb etablieren, fuer mehr Transparenz am Markt sorgen und marktmissbraeuchliche Einflussnahmen auf den Grosshandelspreis verbieten. Die REMIT-Verordnung schafft als erstes unionsrechtliches Regelwerk hierzu einheitliche Vorgaben. Der Band untersucht zunaechst die Transparenz-, Wettbewerbs-, und Aufsichtsstrukturen im Stromgrosshandel vor Erlass der Verordnung. Dabei wird deutlich, wie die Transparenz- und Aufsichtsstrukturen im Stromgrosshandel idealerweise ausgestaltet sein sollten. Auf dieser Grundlage

  11. Electric wiring domestic

    CERN Document Server

    Coker, A J

    1992-01-01

    Electric Wiring: Domestic, Tenth Edition, is a clear and reliable guide to the practical aspects of domestic electric wiring. Intended for electrical contractors, installation engineers, wiremen and students, its aim is to provide essential up to date information on modern methods and materials in a simple, clear, and concise manner. The main changes in this edition are those necessary to bring the work into line with the 16th Edition of the Regulations for Electrical Installations issued by the Institution of Electrical Engineers. The book begins by introducing the basic features of domestic

  12. Upregulation of CREM/ICER suppresses wound endothelial CRE-HIF-1α-VEGF-dependent signaling and impairs angiogenesis in type 2 diabetes

    Directory of Open Access Journals (Sweden)

    Milad S. Bitar

    2015-01-01

    Full Text Available Impaired angiogenesis and endothelial dysfunction in type 2 diabetes constitute dominant risk factors for non-healing wounds and most forms of cardiovascular disease. We propose that diabetes shifts the ‘angiogenic balance’ in favor of an excessive anti-angiogenic phenotype. Herein, we report that diabetes impairs in vivo sponge angiogenic capacity by decreasing VEGF expression and fibrovascular invasion, and reciprocally enhances the formation of angiostatic molecules, such as thrombospondins, NFκB and FasL. Defective in vivo angiogenesis prompted cellular studies in cultured endothelial cells derived from subcutaneous sponge implants (SIECs of control and Goto-Kakizaki rats. Ensuing data from diabetic SIECs demonstrated a marked upregulation in cAMP-PKA-CREB signaling, possibly stemming from increased expression of adenylyl cyclase isoforms 3 and 8, and decreased expression of PDE3. Mechanistically, we found that oxidative stress and PKA activation in diabetes enhanced CREM/ICER expression. This reduces IRS2 cellular content by inhibiting cAMP response element (CRE transcriptional activity. Consequently, a decrease in the activity of Akt-mTOR ensued with a concomitant reduction in the total and nuclear protein levels of HIF-1α. Limiting HIF-1α availability for the specific hypoxia response elements in diabetic SIECs elicited a marked reduction in VEGF expression, both at the mRNA and protein levels. These molecular abnormalities were illustrated functionally by a defect in various pro-angiogenic properties, including cell proliferation, migration and tube formation. A genetic-based strategy in diabetic SIECs using siRNAs against CREM/ICER significantly augmented the PKA-dependent VEGF expression. To this end, the current data identify the importance of CREM/ICER as a negative regulator of endothelial function and establish a link between CREM/ICER overexpression and impaired angiogenesis during the course of diabetes. Moreover, it could

  13. Proglucagon Promoter Cre-Mediated AMPK Deletion in Mice Increases Circulating GLP-1 Levels and Oral Glucose Tolerance.

    Directory of Open Access Journals (Sweden)

    Sophie R Sayers

    Full Text Available Enteroendocrine L-cells synthesise and release the gut hormone glucagon-like peptide-1 (GLP-1 in response to food transit. Deletion of the tumour suppressor kinase LKB1 from proglucagon-expressing cells leads to the generation of intestinal polyps but no change in circulating GLP-1 levels. Here, we explore the role of the downstream kinase AMP-activated protein kinase (AMPK in these cells.Loss of AMPK from proglucagon-expressing cells was achieved using a preproglucagon promoter-driven Cre (iGluCre to catalyse recombination of floxed alleles of AMPKα1 and α2. Oral and intraperitoneal glucose tolerance were measured using standard protocols. L-cell mass was measured by immunocytochemistry. Hormone and peptide levels were measured by electrochemical-based luminescence detection or radioimmunoassay.Recombination with iGluCre led to efficient deletion of AMPK from intestinal L- and pancreatic alpha-cells. In contrast to mice rendered null for LKB1 using the same strategy, mice deleted for AMPK displayed an increase (WT: 0.05 ± 0.01, KO: 0.09±0.02%, p<0.01 in L-cell mass and elevated plasma fasting (WT: 5.62 ± 0.800 pg/ml, KO: 14.5 ± 1.870, p<0.01 and fed (WT: 15.7 ± 1.48pg/ml, KO: 22.0 ± 6.62, p<0.01 GLP-1 levels. Oral, but not intraperitoneal, glucose tolerance was significantly improved by AMPK deletion, whilst insulin and glucagon levels were unchanged despite an increase in alpha to beta cell ratio (WT: 0.23 ± 0.02, KO: 0.33 ± 0.03, p<0.01.AMPK restricts L-cell growth and GLP-1 secretion to suppress glucose tolerance. Targeted inhibition of AMPK in L-cells may thus provide a new therapeutic strategy in some forms of type 2 diabetes.

  14. Self-excising Cre/mutant lox marker recycling system for multiple gene integrations and consecutive gene deletions in Aspergillus oryzae.

    Science.gov (United States)

    Zhang, Silai; Ban, Akihiko; Ebara, Naoki; Mizutani, Osamu; Tanaka, Mizuki; Shintani, Takahiro; Gomi, Katsuya

    2017-04-01

    In this study, we developed a self-excising Cre/loxP-mediated marker recycling system with mutated lox sequences to introduce a number of biosynthetic genes into Aspergillus oryzae. To construct the self-excising marker cassette, both the selectable marker, the Aspergillus nidulans adeA gene, and the Cre recombinase gene (cre), conditionally expressed by the xylanase-encoding gene promoter, were designed to be located between the mutant lox sequences, lox66 and lox71. However, construction of the plasmid failed, possibly owing to a slight expression of cre downstream of the fungal gene promoter in Escherichia coli. Hence, to avoid the excision of the cassette in E. coli, a 71-bp intron of the A. oryzae xynG2 gene was inserted into the cre gene. The A. oryzae adeA deletion mutant was transformed with the resulting plasmid in the presence of glucose, and the transformants were cultured in medium containing xylose as the sole carbon source. PCR analysis of genomic DNA from resultant colonies revealed the excision of both the marker and Cre expression construct, indicating that the self-excising marker cassette was efficient at removing the selectable marker. Using the marker recycling system, hyperproduction of kojic acid could be achieved in A. oryzae by the introduction of two genes that encode oxidoreductase and transporter. Furthermore, we also constructed an alternative marker recycling cassette bearing the A. nidulans pyrithiamine resistant gene (ptrA) as a dominant selectable marker. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  15. Application of valve-regulated lead-acid batteries for storage of solar electricity in stand-alone photovoltaic systems in the northwest areas of China

    Science.gov (United States)

    Hua, Shounan; Zhou, Qingshen; Kong, Delong; Ma, Jianping

    Photovoltaic (PV) installations for solar electric power generation are being established rapidly in the northwest areas of China, and it is increasingly important for these power systems to have reliable and cost effective energy storage. The lead-acid battery is the more commonly used storage technology for PV systems due to its low cost and its wide availability. However, analysis shows that it is the weakest component of PV power systems. Because the batteries can be over discharged, or operated under partial state of charge (PSOC), their service life in PV systems is shorter than could be expected. The working conditions of batteries in remote area installations are worse than those in situations where technical support is readily available. Capacity-loss in lead-acid batteries operated in remote locations often occurs through sulfation of electrodes and stratification of electrolyte. In northwest China, Shandong Sacred Sun Power Sources Industry Co. Ltd. type GFMU valve-regulated lead-acid (VRLA) batteries are being used in PV power stations. These batteries have an advanced grid structure, superior leady paste, and are manufactured using improved plate formation methods. Their characteristics, and their performance in PV systems, are discussed in this paper. The testing results of GFMU VRLA batteries in the laboratory have shown that the batteries could satisfy the demands of the International Electrotechnical Commission (IEC) standards for PV systems.

  16. Control Performance of General Electric Fuel and Torque Regulator Operating on T31-3 Turbine-Propeller Engine in Sea-Level Test Stand

    Science.gov (United States)

    Oppenheimer, Frank L.; Lazar, James

    1951-01-01

    A .General Electric fuel and torque regulator was tested in conjunction with a T31-3 turbine-propeller engine in the sea-level static test stand at the NACA Lewis laboratory. The engine and control were operated over the entire speed range: 11,000 rpm, nominal flight idle, to 13,000 rpm, full power. Steady-state and transient data were recorded and are presented with a description of the four control loops being used in the system. Results of this investigation indicated that single-lever control operation was satisfactory under conditions of test. Transient data presented showed that turbine-outlet temperature did overshoot maximum operating value on acceleration but that the time duration of overshoot did not exceed approximately 1 second. This temperature limiting resulted from a control on fuel flow as a function of engine speed. Speed and torque first reached their desired values 0.4 second from the time of change in power-setting lever position. Maximum speed overshoot was 3 percent.

  17. TRANSFORMATION EFFECTIVENESS FOR ARABIDOPSIS THALIANA PLANTS BY DNA-CONSTRUCTIONS WITH SITE-SPECIFIC RECOMBINASE SYSTEM Cre/loxP

    Directory of Open Access Journals (Sweden)

    A. S. Sekan

    2015-02-01

    Full Text Available Using of new approach with site-specific recombinase system Cre/loxP under the control of 35S-promoter to generate marker-free genetically modified plants was developed. The analysis of recombinase system was carried out during the next generation of Arabidopsis thaliana plants, produced by agrobacterium transformation method. For this purpose two types of DNA-constructions were used for establishing better variant. The histochemical analysis of the plants progeny T1 transformed by both construct types was described. As a result of our work, it was established that the amount of marker-free transformants was arising during every next transformation offspring independently of the used construct type. The new strategy provides a simple and rapid way to eliminate swelective and marker genes.

  18. A 3D Searchable Database of Transgenic Zebrafish Gal4 and Cre Lines for Functional Neuroanatomy Studies.

    Science.gov (United States)

    Marquart, Gregory D; Tabor, Kathryn M; Brown, Mary; Strykowski, Jennifer L; Varshney, Gaurav K; LaFave, Matthew C; Mueller, Thomas; Burgess, Shawn M; Higashijima, Shin-Ichi; Burgess, Harold A

    2015-01-01

    Transgenic methods enable the selective manipulation of neurons for functional mapping of neuronal circuits. Using confocal microscopy, we have imaged the cellular-level expression of 109 transgenic lines in live 6 day post fertilization larvae, including 80 Gal4 enhancer trap lines, 9 Cre enhancer trap lines and 20 transgenic lines that express fluorescent proteins in defined gene-specific patterns. Image stacks were acquired at single micron resolution, together with a broadly expressed neural marker, which we used to align enhancer trap reporter patterns into a common 3-dimensional reference space. To facilitate use of this resource, we have written software that enables searching for transgenic lines that label cells within a selectable 3-dimensional region of interest (ROI) or neuroanatomical area. This software also enables the intersectional expression of transgenes to be predicted, a feature which we validated by detecting cells with co-expression of Cre and Gal4. Many of the imaged enhancer trap lines show intrinsic brain-specific expression. However, to increase the utility of lines that also drive expression in non-neuronal tissue we have designed a novel UAS reporter, that suppresses expression in heart, muscle, and skin through the incorporation of microRNA binding sites in a synthetic 3' untranslated region. Finally, we mapped the site of transgene integration, thus providing molecular identification of the expression pattern for most lines. Cumulatively, this library of enhancer trap lines provides genetic access to 70% of the larval brain and is therefore a powerful and broadly accessible tool for the dissection of neural circuits in larval zebrafish.

  19. Aeromonas hydrophila AmpH and CepH beta-lactamases: derepressed expression in mutants of Escherichia coli lacking creB.

    Science.gov (United States)

    Avison, M B; Niumsup, P; Walsh, T R; Bennett, P M

    2000-11-01

    The class 1 cephalosporinase (CepH) and class 2d oxacillinase (AmpH) from an Aeromonas hydrophila clinical isolate, strain T429125, have been cloned and sequenced. Both enzymes are typical of their equivalents in other species of Aeromonas Both cloned beta-lactamase genes were expressed at a low level in a standard laboratory Escherichia coli strain, but when cloned into a cre deletion E. coli mutant, they were expressed at significantly higher levels. Specific disruption of the creB gene resulted in similar increased levels of beta-lactamase expression, so it was concluded that CreB represses the transcription of ampH and cepH in a cre(+) E. coli strain. The expression of cepH was four times that of ampH in the deltacre mutant because of an additional factor encoded on the cloned T429125 chromosomal fragment containing cepH. This factor was able to trans-activate expression of co-resident ampH in the deltacre mutant such that expression of the two genes was approximately equal. The entire cepH-containing fragment was sequenced, but it contained no genes that were obviously related to any known class of DNA-binding protein.

  20. Excision of a selectable marker gene in transgenic banana using a Cre/lox system controlled by an embryo specific promoter.

    Science.gov (United States)

    Chong-Pérez, Borys; Reyes, Maritza; Rojas, Luis; Ocaña, Bárbara; Ramos, Adolfo; Kosky, Rafael G; Angenon, Geert

    2013-09-01

    Antibiotic and herbicide resistance genes have been used in transgene technology as powerful selection tools. Nonetheless, once transgenic events have been obtained their presence is no longer needed and can even be undesirable. In this work, we have developed a system to excise the selectable marker and the cre recombinase genes from transgenic banana cv. 'Grande Naine' (Musa AAA). To achieve this, the embryo specific REG-2 promoter was isolated from rice and its expression pattern in banana cell clumps, somatic embryos and regenerated plantlets was characterized by using a pREG2::uidA fusion construct. Subsequently, the REG-2 promoter was placed upstream of the cre gene, conferring Cre functionality in somatic embryos and recombination of lox sites resulting in excision of the selectable marker and cre genes. PCR analysis revealed that 41.7 % of the analysed transgenic plants were completely marker free, results that were thereafter confirmed by Southern blot hybridization. These results demonstrate the feasibility of using developmentally controlled promoters to mediate marker excision in banana. This system does not require any extra handling compared to the conventional transformation procedure and might be useful in other species regenerating through somatic embryogenesis.

  1. Multi-step constant-current charging method for electric vehicle, valve-regulated, lead/acid batteries during night time for load-levelling

    Energy Technology Data Exchange (ETDEWEB)

    Ikeya, Tomohiko; Mita, Yuichi; Ishihara, Kaoru [Central Research Inst. of Electric Power Industry, Tokyo (Japan); Sawada, Nobuyuki [Hokkaido Electric Power, Sapporo (Japan); Takagi, Sakae; Murakami, Jun-ichi [Tohoku Electric Power, Sendai (Japan); Kobayashi, Kazuyuki [Tokyo Electric Power, Yokohama (Japan); Sakabe, Tetsuya [Chubu Electric Power, Nagoya (Japan); Kousaka, Eiichi [Hokuriku Electric Power, Toyama (Japan); Yoshioka, Haruki [The Kansai Electric Power, Osaka (Japan); Kato, Satoru [The Chugoku Electric Power, Hiroshima (Japan); Yamashita, Masanori [Shikoku Research Inst., Takamatsu (Japan); Narisoko, Hayato [The Okinawa Electric Power, Naha (Japan); Nishiyama, Kazuo [The Central Electric Power Council, Tokyo (Japan); Adachi, Kazuyuki [Kyushu Electric Power, Fukuoka (Japan)

    1998-09-01

    For the popularization of electric vehicles (EVs), the conditions for charging EV batteries with available current patterns should allow complete charging in a short time, i.e., less than 5 to 8 h. Therefore, in this study, a new charging condition is investigated for the EV valve-regulated lead/acid battery system, which should allow complete charging of EV battery systems with multi-step constant currents in a much shorter time with longer cycle life and higher energy efficiency compared with two-step constant-current charging. Although a high magnitude of the first current in the two-step constant-current method prolongs cycle life by suppressing the softening of positive active material, too large a charging current magnitude degrades cells due to excess internal evolution of heat. A charging current magnitude of approximately 0.5 C is expected to prolong cycle life further. Three-step charging could also increase the magnitude of charging current in the first step without shortening cycle life. Four-or six-step constant-current methods could shorten the charging time to less than 5 h, as well as yield higher energy efficiency and enhanced cycle life of over 400 cycles compared with two-step charging with the first step current of 0.5 C. Investigation of the degradation mechanism of the batteries revealed that the conditions of multi-step constant-current charging suppressed softening of positive active material and sulfation of negative active material, but, unfortunately, advanced the corrosion of the grids in the positive plates. By adopting improved grids and cooling of the battery system, the multistep constant-current method may enhance the cycle life. (orig.)

  2. Deregulation of Electricity Market and Drivers of Demand for Electrical Energy in Industry

    OpenAIRE

    Bojnec Štefan; Papler Drago

    2016-01-01

    This paper investigates deregulation of electricity market focusing on electricity prices and drivers of demand for electrical energy in industry in Slovenia. The patterns in evolution of real electricity price developments and the three main components of the electricity price are calculated: liberalized market share for purchased electricity price, regulated infrastructure share for use of electricity network grids and mandatory state charges in the sale of electricity (duty, excise duty an...

  3. α-Fetoprotein promoter-driven Cre/LoxP-switched RNA interference for hepatocellular carcinoma tissue-specific target therapy.

    Directory of Open Access Journals (Sweden)

    Yuan-Fei Peng

    Full Text Available RNA interference (RNAi has recently emerged as a potential treatment modality for hepatocellular carcinoma (HCC therapy, but the lack of cellular targets and sustained efficacy limits its application. The purpose of this study is to develop an HCC tissue-specific RNAi system and investigate its possibility for HCC treatment.Two different HCC-specific RNAi systems in which therapeutic miRNA or shRNA against target gene (Beclin 1 was directly or indirectly driven by alpha-fetoprotein promoter (AFP-miRNA and AFP-Cre/LoxP-shRNA were constructed. Human HCC cell lines (HepG2, Hep3B and HCCLM3 and non-HCC cell lines (L-02, Hela and SW1116 were infected with the systems. The effectiveness and tissue-specificity of the systems were examined by Q-PCR and western blot analysis. The efficacy of the systems was further tested in mouse model of HCC by intravenous or intratumoral administration. The feasibility of the system for HCC treatment was evaluated by applying the system as adjuvant therapy to enhance sorafenib treatment. An AFP-Cre/LoxP-shRNA system targeting Atg5 gene (AFP-Cre/LoxP-shRNA-Atg5 was constructed and its efficacy in sensitizing HCC cells (MHCC97L/PLC to sorafenib treatment was examined by apoptosis assay in vitro and tumorigenesis assay in vivo.The AFP-miRNA system could silence target gene (Beclin 1 but required a high titer which was lethal to target cells. The AFP-Cre/LoxP-shRNA system could efficiently knockdown target gene while maintain high HCC specificity. Intratumoral injection of the AFP-Cre/LoxP-shRNA system could efficiently silence target gene (Beclin 1 in vivo while intravenous administration could not. The AFP-Cre/LoxP-shRNA system target Atg5 gene could significantly sensitize MHCC97L/PLC cells to sorafenib-induced apoptosis in vitro and tumor growth suppression in vivo.An efficient HCC tissue-specific RNAi system (AFP-Cre/LoxP-shRNA was successfully established. The system provides a usable tool for HCC-specific RNAi

  4. Mikroskop görüntüsünde otomatik embriyonik kök hücre tespiti ve sayımı

    Directory of Open Access Journals (Sweden)

    Gökçen Çetinel

    2016-08-01

    Full Text Available Mikroskop altında hücre sayımı, bir uzman tarafından mikroskop merceğine sürekli bakılarak veya otomatik hücre sayımı yöntemleri kullanılarak yapılabilmektedir. Sayım uzman tarafından yapıldığında oldukça yorucu, uzun süren ve düşük doğruluğa sahip bir işlem haline gelmektedir. Bunun dışında hücre sayımını zorlaştrıan ve doğruluğu düşüren başka etkenler de mevcuttur. Bu nedenle, hücre sayımının otomatik bir şekilde yapılması ve sunulan sayım yöntemlerinin iyileştirilmesi gerekmektedir. Bu çalışmada, flüoresans mikroskop görüntüsünde otomatik hücre tespiti ve sayımı için bir yöntem sunulmuştur. Yöntemin tüm adımları açıklanmıştır. Sunulan yöntemin etkinliği birçok farklı durum için simülasyon programları vasıtasıyla test edilmiş, yöntemin başarıya ulaştığı ve gelecek vadeden bir çalışma olduğu gösterilmiştir.

  5. Electrical applications 2

    CERN Document Server

    Tyler, David W

    1998-01-01

    Electrical Applications 2 covers the BTEC NII level objectives in Electrical Applications U86/330. To understand the applications, a knowledge of the underlying principles is needed and these are covered briefly in the text. Key topics discussed are: the transmission and distribution of electrical energy; safety and regulations; tariffs and power factor correction; materials and their applications in the electrical industry; transformers; DC machines; illumination; and fuse protection. Included in each chapter are worked examples which should be carefully worked through before progressing to t

  6. Regulation of Energy Balance via BDNF Expressed in Nonparaventricular Hypothalamic Neurons.

    Science.gov (United States)

    Yang, Haili; An, Juan Ji; Sun, Chao; Xu, Baoji

    2016-05-01

    Brain-derived neurotrophic factor (BDNF) expressed in the paraventricular hypothalamus (PVH) has been shown to play a key role in regulating energy intake and energy expenditure. BDNF is also expressed in other hypothalamic nuclei; however, the role in the control of energy balance for BDNF produced in these structures remains largely unknown. We found that deleting the Bdnf gene in the ventromedial hypothalamus (VMH) during embryogenesis using the Sf1-Cre transgene had no effect on body weight in mice. In contrast, deleting the Bdnf gene in the adult VMH using Cre-expressing virus led to significant hyperphagia and obesity. These observations indicate that the lack of a hyperphagia phenotype in the Sf1-Cre/Bdnf mutant mice is likely due to developmental compensation. To investigate the role of BDNF expressed in other hypothalamic areas, we employed the hypothalamus-specific Nkx2.1-Cre transgene to delete the Bdnf gene. We found that the Nkx2.1-Cre transgene could abolish BDNF expression in many hypothalamic nuclei, but not in the PVH, and that the resulting mutant mice developed modest obesity due to reduced energy expenditure. Thus, BDNF produced in the VMH plays a role in regulating energy intake. Furthermore, BDNF expressed in hypothalamic areas other than PVH and VMH is also involved in the control of energy expenditure.

  7. Transcriptome analysis of temporal regulation of carbon metabolism by CcpA in Bacillus subtilis reveals additional target genes

    NARCIS (Netherlands)

    Lulko, Andrzej T.; Buist, Girbe; Kok, Jan; Kuipers, Oscar P.

    2007-01-01

    The pleiotropic regulator of carbon metabolism in Grampositive bacteria, CcpA, regulates gene expression by binding to so-called cre elements, which are located either upstream or in promoter regions, or in open-reading frames. In this study we compared the transcriptomes of Bacillus subtilis 168

  8. Growth factor independence 1b (gfi1b is important for the maturation of erythroid cells and the regulation of embryonic globin expression.

    Directory of Open Access Journals (Sweden)

    Lothar Vassen

    Full Text Available Growth factor independence 1b (GFI1B is a DNA binding repressor of transcription with vital functions in hematopoiesis. Gfi1b-null embryos die at midgestation very likely due to defects in erythro- and megakaryopoiesis. To analyze the full functionality of Gfi1b, we used conditionally deficient mice that harbor floxed Gfi1b alleles and inducible (Mx-Cre, Cre-ERT or erythroid specific (EpoR-Cre Cre expressing transgenes. In contrast to the germline knockout, EpoR-Cre mediated erythroid specific ablation of Gfi1b allows full gestation, but causes perinatal lethality with very few mice surviving to adulthood. Both the embryonic deletion of Gfi1b by EpoR-Cre and the deletion in adult mice by Mx-Cre or Cre-ERT leads to reduced numbers of erythroid precursors, perturbed and delayed erythroid maturation, anemia and extramedullary erythropoiesis. Global expression analyses showed that the Hba-x, Hbb-bh1 and Hbb-y embryonic globin genes were upregulated in Gfi1b deficient TER119+ fetal liver cells over the gestation period from day 12.5-17.5 p.c. and an increased level of Hbb-bh1 and Hbb-y embryonic globin gene expression was even maintained in adult Gfi1b deficient mice. While the expression of Bcl11a, a regulator of embryonic globin expression was not affected by Gfi1b deficiency, the expression of Gata1 was reduced and the expression of Sox6, also involved in globin switch, was almost entirely lost when Gfi1b was absent. These findings establish Gfi1b as a regulator of embryonic globin expression and embryonic and adult erythroid maturation.

  9. A 3D searchable database of transgenic zebrafish Gal4 and Cre lines for functional neuroanatomy studies

    Directory of Open Access Journals (Sweden)

    Gregory D Marquart

    2015-11-01

    Full Text Available Transgenic methods enable the selective manipulation of neurons for functional mapping of neuronal circuits. Using confocal microscopy, we have imaged the cellular-level expression of 109 transgenic lines in live 6 day post fertilization larvae, including 80 Gal4 enhancer trap lines, 9 Cre enhancer trap lines and 20 transgenic lines that express fluorescent proteins in defined gene-specific patterns. Image stacks were acquired at single micron resolution, together with a broadly expressed neural marker, which we used to align enhancer trap reporter patterns into a common 3-dimensional reference space. To facilitate use of this resource, we have written software that enables searching for transgenic lines that label cells within a selectable 3-dimensional region of interest or neuroanatomical area. Many of the imaged enhancer trap lines show intrinsic brain-specific expression. However, to increase the utility of lines that also drive expression in non-neuronal tissue we have designed a novel UAS reporter, that suppresses expression in heart, muscle and skin through the incorporation of microRNA binding sites in a synthetic 3' untranslated region. Finally, we mapped the site of transgene integration, thus providing molecular identification of the expression pattern for most lines. Cumulatively, this library of enhancer trap lines provides genetic access to 70 % of the larval brain and is therefore a powerful and broadly accessible tool for the dissection of neural circuits in larval zebrafish.

  10. A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination

    Directory of Open Access Journals (Sweden)

    Vassetzky Yegor S

    2008-12-01

    Full Text Available Abstract Background Introduction of new antibiotic resistance genes in the plasmids of interest is a frequent task in molecular cloning practice. Classical approaches involving digestion with restriction endonucleases and ligation are time-consuming. Findings We have created a set of insertion vectors (pINS carrying genes that provide resistance to various antibiotics (puromycin, blasticidin and G418 and containing a loxP site. Each vector (pINS-Puro, pINS-Blast or pINS-Neo contains either a chloramphenicol or a kanamycin resistance gene and is unable to replicate in most E. coli strains as it contains a conditional R6Kγ replication origin. Introduction of the antibiotic resistance genes into the vector of interest is achieved by Cre-mediated recombination between the replication-incompetent pINS and a replication-competent target vector. The recombination mix is then transformed into E. coli and selected by the resistance marker (kanamycin or chloramphenicol present in pINS, which allows to recover the recombinant plasmids with 100% efficiency. Conclusion Here we propose a simple strategy that allows to introduce various antibiotic-resistance genes into any plasmid containing a replication origin, an ampicillin resistance gene and a loxP site.

  11. Study of muscle cell dedifferentiation after skeletal muscle injury of mice with a Cre-Lox system.

    Directory of Open Access Journals (Sweden)

    Xiaodong Mu

    2011-02-01

    Full Text Available Dedifferentiation of muscle cells in the tissue of mammals has yet to be observed. One of the challenges facing the study of skeletal muscle cell dedifferentiation is the availability of a reliable model that can confidentially distinguish differentiated cell populations of myotubes and non-fused mononuclear cells, including stem cells that can coexist within the population of cells being studied.In the current study, we created a Cre/Lox-β-galactosidase system, which can specifically tag differentiated multinuclear myotubes and myotube-generated mononuclear cells based on the activation of the marker gene, β-galactosidase. By using this system in an adult mouse model, we found that β-galactosidase positive mononuclear cells were generated from β-galactosidase positive multinuclear myofibers upon muscle injury. We also demonstrated that these mononuclear cells can develop into a variety of different muscle cell lineages, i.e., myoblasts, satellite cells, and muscle derived stem cells.These novel findings demonstrated, for the first time, that cellular dedifferentiation of skeletal muscle cells actually occurs in mammalian skeletal muscle following traumatic injury in vivo.

  12. Cell-Type-Specific Circuit Connectivity of Hippocampal CA1 Revealed through Cre-Dependent Rabies Tracing

    Directory of Open Access Journals (Sweden)

    Yanjun Sun

    2014-04-01

    Full Text Available We developed and applied a Cre-dependent, genetically modified rabies-based tracing system to map direct synaptic connections to specific CA1 neuron types in the mouse hippocampus. We found common inputs to excitatory and inhibitory CA1 neurons from CA3, CA2, the entorhinal cortex (EC, the medial septum (MS, and, unexpectedly, the subiculum. Excitatory CA1 neurons receive inputs from both cholinergic and GABAergic MS neurons, whereas inhibitory neurons receive a great majority of inputs from GABAergic MS neurons. Both cell types also receive weaker input from glutamatergic MS neurons. Comparisons of inputs to CA1 PV+ interneurons versus SOM+ interneurons showed similar strengths of input from the subiculum, but PV+ interneurons received much stronger input than SOM+ neurons from CA3, the EC, and the MS. Thus, rabies tracing identifies hippocampal circuit connections and maps how the different input sources to CA1 are distributed with different strengths on each of its constituent cell types.

  13. Using the cre-lox recombination system to assess functional impairment caused by amino acid substitutions in yeast proteins

    Directory of Open Access Journals (Sweden)

    Shirley Renee L.

    2004-01-01

    Full Text Available A method was developed to assess the functional significance of a sequence motif in yeast Upf3p, a protein required for nonsense-mediated mRNA decay (NMD. The motif lies at the edge of the Upf3p-Upf2p interaction domain, but at the same time resembles the canonical leucine-rich nuclear export sequence (NES found in proteins that bind Crm1p exportin. To test the function of the putative NES, site-directed mutations that cause substitutions of conserved NES-A residues were first selected to identify hypermorphic alleles. Next, a portable Crm1p-binding NES from HIV-1 Rev protein that functions in yeast was fused en masse to the C-terminus of variant Upf3 proteins using loxP sites recognized by bacterial cre-recombinase. Finally, variant Upf3-Rev proteins that were functional in NMD were selected and examined for the types of amino acid substitutions present in NES-A. The mutational analysis revealed that amino acid substitutions in the Upf3 NES impair both nuclear export and the Upf2p-Upf3p interaction, both of which are required for Upf3p to function in NMD. The method described in this report could be modified for the genetic analysis of a variety of portable protein domains.

  14. CRE Solvability, Nonlocal Symmetry and Exact Interaction Solutions of the Fifth-Order Modified Korteweg-de Vries Equation

    Science.gov (United States)

    Cheng, Wen-Guang; Qiu, De-Qin; Yu, Bo

    2017-06-01

    This paper is concerned with the fifth-order modified Korteweg-de Vries (fmKdV) equation. It is proved that the fmKdV equation is consistent Riccati expansion (CRE) solvable. Three special form of soliton-cnoidal wave interaction solutions are discussed analytically and shown graphically. Furthermore, based on the consistent tanh expansion (CTE) method, the nonlocal symmetry related to the consistent tanh expansion (CTE) is investigated, we also give the relationship between this kind of nonlocal symmetry and the residual symmetry which can be obtained with the truncated Painlevé method. We further study the spectral function symmetry and derive the Lax pair of the fmKdV equation. The residual symmetry can be localized to the Lie point symmetry of an enlarged system and the corresponding finite transformation group is computed. Supported by National Natural Science Foundation of China under Grant No. 11505090, and Research Award Foundation for Outstanding Young Scientists of Shandong Province under Grant No. BS2015SF009

  15. Electricity Customers

    Science.gov (United States)

    This page discusses key sectors and how they use electricity. Residential, commercial, and industrial customers each account for roughly one-third of the nation’s electricity use. The transportation sector also accounts for a small fraction of electricity.

  16. Numb regulates acinar cell dedifferentiation and survival during pancreatic damage and acinar-to-ductal metaplasia.

    Science.gov (United States)

    Greer, Renee L; Staley, Binnaz K; Liou, Angela; Hebrok, Matthias

    2013-11-01

    Pancreatic ductal adenocarcinoma (PDA) is a leading cause of cancer-related death. Through the process of acinar-to-ductal metaplasia (ADM), pancreatic acinar cells give rise to pancreatic intraepithelial neoplasia (PanIN), the most common precursor of PDA. However, even when Kras is activated in a majority of acinar cells, ADM and subsequent development of PanINs is inefficient in the absence of additional stresses. Numb regulates cell junctions, integrins, and the activity of embryonic signaling pathways; therefore, we investigated its effects on acinar cell dedifferentiation, regeneration, and metaplasia. We used mouse models of pancreatic regeneration and PDA as well as mice with loss-of-function alleles of Numb (p48Cre/p48Cre(ER);Numb(f/f) and p48Cre/p48Cre(ER);Kras(G12D);Numb(f/f) mice) to study the roles of Numb in pancreatic regeneration and ADM. Loss of Numb resulted in premature dedifferentiation of acinar cells in response to injury due to administration of the cholecystokinin analogue cerulein and interfered with acinar cell regeneration. Numb was found to regulate multiple signaling pathways in acinar cells during cerulein-induced pancreatitis. Disruption of Numb accelerated and destabilized ADM in the context of oncogenic Kras (in p48Cre;Kras(G12D);Numb(f/f) and p48Cre(ER);Kras(G12D);Numb(f/f) mice). Numb is an important regulator of acinar cell differentiation and viability during metaplasia. In mice with pancreatitis or pancreatic injury, elimination of Numb causes dedifferentiated acinar cells to undergo apoptosis, and this is not mitigated by oncogenic Kras. Copyright © 2013 AGA Institute. Published by Elsevier Inc. All rights reserved.

  17. Talk from M. Jean Syrota, head of the French commission of electricity regulation; Intervention de M. Jean Syrota, president de la Commission de Regulation de l'Electricite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    In his talk, J. Syrota makes first a status of the opening of the electricity market in France and in Europe (degree of competition, conditions for an effective opening of the market, the most convenient type of market). Then, he discusses the conciliation between the opening of the market and the public utility mission, and the security of electricity supplies in an opened market. (J.S.)

  18. Hypoxia inducible factor-2α regulates the development of retinal astrocytic network by maintaining adequate supply of astrocyte progenitors.

    Directory of Open Access Journals (Sweden)

    Li-Juan Duan

    Full Text Available Here we investigate the role of hypoxia inducible factor (HIF-2α in coordinating the development of retinal astrocytic and vascular networks. Three Cre mouse lines were used to disrupt floxed Hif-2α, including Rosa26(CreERT2, Tie2(Cre, and GFAP(Cre. Global Hif-2α disruption by Rosa26(CreERT2 led to reduced astrocytic and vascular development in neonatal retinas, whereas endothelial disruption by Tie2(Cre had no apparent effects. Hif-2α deletion in astrocyte progenitors by GFAP(Cre significantly interfered with the development of astrocytic networks, which failed to reach the retinal periphery and were incapable of supporting vascular development. Perplexingly, the abundance of strongly GFAP(+ mature astrocytes transiently increased at P0 before they began to lag behind the normal controls by P3. Pax2(+ and PDGFRα(+ astrocytic progenitors and immature astrocytes were dramatically diminished at all stages examined. Despite decreased number of astrocyte progenitors, their proliferation index or apoptosis was not altered. The above data can be reconciled by proposing that HIF-2α is required for maintaining the supply of astrocyte progenitors by slowing down their differentiation into non-proliferative mature astrocytes. HIF-2α deficiency in astrocyte progenitors may accelerate their differentiation into astrocytes, a change which greatly interferes with the replenishment of astrocyte progenitors due to insufficient time for proliferation. Rapidly declining progenitor supply may lead to premature cessation of astrocyte development. Given that HIF-2α protein undergoes oxygen dependent degradation, an interesting possibility is that retinal blood vessels may regulate astrocyte differentiation through their oxygen delivery function. While our findings support the consensus that retinal astrocytic template guides vascular development, they also raise the possibility that astrocytic and vascular networks may mutually regulate each other

  19. Cyclic strain causes heterogeneous induction of transcription factors, AP-1, CRE binding protein and NF-kB, in endothelial cells: species and vascular bed diversity.

    Science.gov (United States)

    Du, W; Mills, I; Sumpio, B E

    1995-12-01

    Recent studies demonstrate that cyclic strain stimulates protein kinase C in bovine aortic endothelial cells (BAEC) as well as the induction of immediate early genes and the transcription factor activator protein-1 (AP-1) in human umbilical vein endothelial cells (HUVEC). The objective of this study was to determine whether transcriptional factor induction in endothelial cells (EC) exposed to strain is the same with regard to the species and vascular bed they are derived from. Evidence for a heterogeneous response for growth, orientation and prostacyclin secretion has been obtained for a variety of EC exposed to cyclic strain. In this study, we investigated cyclic strain mediated induction of transcription factors, AP-1, cAMP response element binding protein (CRE) and nuclear factor kB (NF-kB) in cultured EC from HUVEC, human aorta (HAEC), and BAEC. EC were exposed to 10% average strain at 60 cpm for up to 24 h. At varying time points, nuclear protein was extracted and analyzed for production of AP-1, CRE and NF-kB by electromobility shift assay. The results demonstrate that EC exposure to cyclic strain leads to a significant induction of AP-1, CRE and NF-kB in HAEC and HUVEC, but not in BAEC. Furthermore, these findings are in marked contrast to the previously described shear stress induced activation of AP-1 and NF-kB in BAEC. There was also a temporal difference in their response such that stretch-induced activation of AP-1 and NF-kB peaked at 4 h, whereas CRE increased in a biphasic manner at 15 min and 24 h. These results may partially explain the divergent effects of cyclic strain on EC gene expression and phenotype in EC from different vascular beds and species and underscore the difference in EC response to cyclic strain and shear stress.

  20. Heat shock induced excision of selectable marker genes in transgenic banana by the Cre-lox site-specific recombination system.

    Science.gov (United States)

    Chong-Pérez, Borys; Kosky, Rafael G; Reyes, Maritza; Rojas, Luis; Ocaña, Bárbara; Tejeda, Marisol; Pérez, Blanca; Angenon, Geert

    2012-06-30

    Selectable marker genes are indispensable for efficient production of transgenic events, but are no longer needed after the selection process and may cause public concern and technological problems. Although several gene excision systems exist, few have been optimized for vegetatively propagated crops. Using a Cre-loxP auto-excision strategy, we obtained transgenic banana plants cv. Grande Naine (Musa AAA) devoid of the marker gene used for selection. We used T-DNA vectors with the cre recombinase gene under control of a heat shock promoter and selectable marker gene cassettes placed between two loxP sites in direct orientation, and a gene of interest inserted outside of the loxP sites. Heat shock promoters pGmHSP17.6-L and pHSP18.2, from soybean and Arabidopsis respectively, were tested. A transient heat shock treatment of primary transgenic embryos was sufficient for inducing cre and excising cre and the marker genes. Excision efficiency, as determined by PCR and Southern hybridization was 59.7 and 40.0% for the GmHSP17.6-L and HSP18.2 promoters, respectively. Spontaneous excision was not observed in 50 plants derived from untreated transgenic embryos. To our knowledge this is the first report describing an efficient marker gene removal system for banana. The method described is simple and might be generally applicable for the production of marker-free transgenic plants of many crop species. Copyright © 2011 Elsevier B.V. All rights reserved.

  1. Foxc2CreERT2knock-in mice mark stage-specific Foxc2-expressing cells during mouse organogenesis.

    Science.gov (United States)

    Amin, Mohammed Badrul; Miura, Naoyuki; Uddin, Mohammad Khaja Mafij; Islam, Mohammod Johirul; Yoshida, Nobuaki; Iseki, Sachiko; Kume, Tsutomu; Trainor, Paul A; Saitsu, Hirotomo; Aoto, Kazushi

    2017-01-01

    Foxc2, a member of the winged helix transcription factor family, is essential for eye, calvarial bone, cardiovascular and kidney development in mice. Nevertheless, how Foxc2-expressing cells and their descendent cells contribute to the development of these tissues and organs has not been elucidated. Here, we generated a Foxc2 knock-in (Foxc2 CreERT2 ) mouse, in which administration of estrogen receptor antagonist tamoxifen induces nuclear translocation of Cre recombinase in Foxc2-expressing cells. By crossing with ROSA-LacZ reporter mice (Foxc2 CreERT2 ; R26R), the fate of Foxc2 positive (Foxc2 + ) cells was analyzed through LacZ staining at various embryonic stages. We found Foxc2 + cell descendants in the supraoccipital and exoccipital bone in E18.5 embryos, when tamoxifen was administered at embryonic day (E) 8.5. Furthermore, Foxc2 + descendant cranial neural crest cells at E8-10 were restricted to the corneal mesenchyme, while Foxc2 + cell derived cardiac neural crest cells at E6-12 were found in the aorta, pulmonary trunk and valves, and endocardial cushions. Foxc2 + cell descendant contributions to the glomerular podocytes in the kidney were also observed following E6.5 tamoxifen treatment. Our results are consistent with previous reports of Foxc2 expression during early embryogenesis and the Foxc2 CreERT2 mouse provides a tool to investigate spatiotemporal roles of Foxc2 and contributions of Foxc2 + expressing cells during mouse embryogenesis. © 2016 Japanese Teratology Society.

  2. A Trp53fl/flPtenfl/fl mouse model of undifferentiated pleomorphic sarcoma mediated by adeno-Cre injection and in vivo bioluminescence imaging.

    Directory of Open Access Journals (Sweden)

    Marisa R Buchakjian

    Full Text Available Genetic mouse models of soft tissue sarcoma provide critical insights into disease pathophysiology, which are oftentimes unable to be extracted from human tumor samples or xenograft models. In this study we describe a mouse model of soft tissue sarcoma mediated by adenoviral-Cre recombinase injection into Trp53fl/fl/Ptenfl/fl lox-stop-lox luciferase mice. Injection of adenovirus expressing Cre recombinase, either subcutaneously or intramuscularly in two experimental groups, results in viral infection and gene recombination with 100% penetrance within the first 24 hours following injection. Luciferase expression measured by real-time bioluminescence imaging increases over time, with an initial robust increase following viral injection, followed by a steady rise over the next several weeks as primary tumors develop and grow. Intramuscular injections were more commonly associated with evidence of systemic viral distribution than subcutaneous injections. All mice developed soft tissue sarcomas at the primary injection site, with histological examination identifying 93% of tumors as invasive pleomorphic sarcomas based on microscopic morphology and immunohistochemical expression of sarcoma markers. A lymphocytic infiltrate was present in 64% of the sarcomas in this immunocompetent model and 71% of tumors expressed PD-L1. This is the first report of a viral-Cre mediated Trp53/Pten mouse model of undifferentiated pleomorphic sarcoma. The bioluminescence imaging feature, along with high penetrance of the model and its immunological characteristics, makes it suited for pre-clinical studies of soft tissue sarcoma.

  3. High-resolution labeling and functional manipulation of specific neuron types in mouse brain by Cre-activated viral gene expression.

    Directory of Open Access Journals (Sweden)

    Sandra J Kuhlman

    2008-04-01

    Full Text Available We describe a method that combines Cre-recombinase knockin mice and viral-mediated gene transfer to genetically label and functionally manipulate specific neuron types in the mouse brain. We engineered adeno-associated viruses (AAVs that express GFP, dsRedExpress, or channelrhodopsin (ChR2 upon Cre/loxP recombination-mediated removal of a transcription-translation STOP cassette. Fluorescent labeling was sufficient to visualize neuronal structures with synaptic resolution in vivo, and ChR2 expression allowed light activation of neuronal spiking. The structural dynamics of a specific class of neocortical neuron, the parvalbumin-containing (Pv fast-spiking GABAergic interneuron, was monitored over the course of a week. We found that although the majority of Pv axonal boutons were stable in young adults, bouton additions and subtractions on axonal shafts were readily observed at a rate of 10.10% and 9.47%, respectively, over 7 days. Our results indicate that Pv inhibitory circuits maintain the potential for structural re-wiring in post-adolescent cortex. With the generation of an increasing number of Cre knockin mice and because viral transfection can be delivered to defined brain regions at defined developmental stages, this strategy represents a general method to systematically visualize the structure and manipulate the function of different cell types in the mouse brain.

  4. Targeted deletion of Sox10 by Wnt1-cre defects neuronal migration and projection in the mouse inner ear.

    Directory of Open Access Journals (Sweden)

    YanYan Mao

    Full Text Available Sensory nerves of the brainstem are mostly composed of placode-derived neurons, neural crest-derived neurons and neural crest-derived Schwann cells. This mixed origin of cells has made it difficult to dissect interdependence for fiber guidance. Inner ear-derived neurons are known to connect to the brain after delayed loss of Schwann cells in ErbB2 mutants. However, the ErbB2 mutant related alterations in the ear and the brain compound interpretation of the data. We present here a new model to evaluate exclusively the effect of Schwann cell loss on inner ear innervation. Conditional deletion of the neural crest specific transcription factor, Sox10, using the rhombic lip/neural crest specific Wnt1-cre driver spares Sox10 expression in the ear. We confirm that neural crest-derived cells provide a stop signal for migrating spiral ganglion neurons. In the absence of Schwann cells, spiral ganglion neurons migrate into the center of the cochlea and even out of the ear toward the brain. Spiral ganglion neuron afferent processes reach the organ of Corti, but many afferent fibers bypass the organ of Corti to enter the lateral wall of the cochlea. In contrast to this peripheral disorganization, the central projection to cochlear nuclei is normal. Compared to ErbB2 mutants, conditional Sox10 mutants have limited cell death in spiral ganglion neurons, indicating that the absence of Schwann cells alone contributes little to the embryonic survival of neurons. These data suggest that neural crest-derived cells are dispensable for all central and some peripheral targeting of inner ear neurons. However, Schwann cells provide a stop signal for migratory spiral ganglion neurons and facilitate proper targeting of the organ of Corti by spiral ganglion afferents.

  5. Diphtheria toxin treatment of Pet-1-Cre floxed diphtheria toxin receptor mice disrupts thermoregulation without affecting respiratory chemoreception

    Science.gov (United States)

    Cerpa, Verónica; Gonzalez, Amalia; Richerson, George B.

    2014-01-01

    In genetically-modified Lmx1bf/f/p mice, selective deletion of LMX1B in Pet-1 expressing cells leads to failure of embryonic development of serotonin (5-HT) neurons. As adults, these mice have a decreased hypercapnic ventilatory response and abnormal thermoregulation. This mouse model has been valuable in defining the normal role of 5-HT neurons, but it is possible that developmental compensation reduces the severity of observed deficits. Here we studied mice genetically modified to express diphtheria toxin receptors (DTR) on Pet-1 expressing neurons (Pet-1-Cre/Floxed DTR or Pet1/DTR mice). These mice developed with a normal complement of 5-HT neurons. As adults, systemic treatment with 2 – 35 μg diphtheria toxin (DT) reduced the number of tryptophan hydroxylase immunoreactive (TpOH-ir) neurons in the raphe nuclei and ventrolateral medulla by 80%. There were no effects of DT on baseline ventilation (VE) or the ventilatory response to hypercapnia or hypoxia. At an ambient temperature (TA) of 24°C, all Pet1/DTR mice dropped their body temperature (TB) below 35°C after DT treatment, but the latency was shorter in males than females (3.0 ± 0.37 vs 4.57 ± 0.29 days, respectively; p thermoregulation, in males more than females. In comparison to models with deficient embryonic development of 5-HT neurons, acute deletion of 5-HT neurons in adults leads to a greater defect in thermoregulation, suggesting that significant developmental compensation can occur. PMID:25171790

  6. How can Cre.eight – an online t-shirt brand – increase brand awareness and sales, in Portuguese market, through its main social media networking sites : Facebook and Instagram?

    OpenAIRE

    Ferraz, Pedro Filgueiras Soares

    2016-01-01

    Cre.eight is an online t-shirt brand founded by Pedro Ferraz in 2015. This dissertation has the objective of studying Cre.eight and understanding the importance of Facebook and Instagram as means of increasing brand awareness and sales. The relevance of social media networking sites for the millennial generation was evaluated, along with the tools that these platforms posses for up-rising brands. An outlined analysis of Facebook and Instagram and their power as communication ch...

  7. ERK phosphorylation regulates sleep and plasticity in Drosophila.

    Directory of Open Access Journals (Sweden)

    William M Vanderheyden

    Full Text Available Given the relationship between sleep and plasticity, we examined the role of Extracellular signal-regulated kinase (ERK in regulating baseline sleep, and modulating the response to waking experience. Both sleep deprivation and social enrichment increase ERK phosphorylation in wild-type flies. The effects of both sleep deprivation and social enrichment on structural plasticity in the LNvs can be recapitulated by expressing an active version of ERK (UAS-ERK(SEM pan-neuronally in the adult fly using GeneSwitch (Gsw Gsw-elav-GAL4. Conversely, disrupting ERK reduces sleep and prevents both the behavioral and structural plasticity normally induced by social enrichment. Finally, using transgenic flies carrying a cAMP response Element (CRE-luciferase reporter we show that activating ERK enhances CRE-Luc activity while disrupting ERK reduces it. These data suggest that ERK phosphorylation is an important mediator in transducing waking experience into sleep.

  8. Report of the Economy, Sustainable Development and Land Planning Commission aiming at authorizing electricity final user and small companies to go back to the electricity regulated price; Rapport fait au nom de la commission de l'economie, du developpement durable et de l'amenagement du territoire (1) sur la proposition de loi de MM. Ladislas PONIATOWSKI, et tendant a autoriser les consommateurs finals domestiques d'electricite et les petites entreprises a retourner au tarif reglemente d'electricite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-07-01

    This report first presents the French regulated price system by recalling the legal bases for electricity and natural gas pricing, and by describing the progressive process of the electricity and natural gas market opening in France. It outlines that a reversibility principle has been introduced along with regulated pricing in most of the European Union countries. It also comments the complexity created on this issue by successive laws in France, the consequences of the soon coming law on the new organization of the electricity market. Then, the report comments the proposition which aims at authorizing electricity household users and small companies to go back to the regulated electricity price, thereby perpetuating the reversibility principle, while maintaining a criterion of installed electricity power, including natural gas prices and new consumption sites. A table proposes a comparison between existing texts, the present law project and this Commission proposition.

  9. CAPM (Capital Asset Pricing Model) and regulation in Brazilian electric distribution sector; CAPM (Capital Asset Pricing Model) e regulacao no segmento de distribuicao do setor eletrico brasileiro

    Energy Technology Data Exchange (ETDEWEB)

    Pinto, Rinaldo Caldeira; Parente, Virginia [Universidade de Sao Paulo (USP), SP (Brazil)], emails: rinaldo@iee.usp.br, vparente@iee.usp.br

    2010-07-01

    The aim of this paper is to analyse the use of Capital Asset Pricing Model (CAPM) Beta in the Brazilian electric distribution sector tariffs review. The betas applied by the Regulatory Agency are defined using data from the American, English and Brazilian markets. These betas will then be compared to the betas obtained in the domestic market. The betas were directly obtained from an economic-financial databank largely employed by the market. The sample is composed of companies' shares, priced at Sao Paulo Stock Market. Their main activity is the distribution of electric energy between July 2002 and July 2007. The results of mean betas obtained for the distribution segment, with values close to the ones applied by the regulatory agency for the cycle of tariff reviews between 2007-2010. (author)

  10. Variables contributing to an excellent customer service management profile within the regulated electric utility industry: A comparison of self-concept with customer satisfaction for customer service management

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, L.E.

    1991-01-01

    This research sought to address the relationship between self-concept and customer satisfaction: can customer satisfaction with a major electric utility be explained in terms of the self-reported, self-concept of the utility's managers The population to which the results of this study were generalized consisted of customer service managers in public electric utilities across the United States. In order to represent this population, a sample was selected consisting of customer service managers at a midwestern electric utility based in a large metropolitan area. Participants in this study were managers of four direct customer contact service organizations within six geographic division organizations. The methodology included comparisons of these four customer contact service organizations on twelve independent, self-concept variables and six customer satisfaction dependent variables using Analysis of Variance (ANOVA), Scheffe' tests, Chi-Square, and Stepwise multiple regression. The groups were found not to be significantly different and knowledge of the self-concept scores for managers will not increase the ability to predict customer satisfaction over no knowledge of self-concept scores.

  11. Onboard Hydrogen/Helium Sensors in Support of the Global Technical Regulation: An Assessment of Performance in Fuel Cell Electric Vehicle Crash Tests

    Energy Technology Data Exchange (ETDEWEB)

    Post, M. B.; Burgess, R.; Rivkin, C.; Buttner, W.; O' Malley, K.; Ruiz, A.

    2012-09-01

    Automobile manufacturers in North America, Europe, and Asia project a 2015 release of commercial hydrogen fuel cell powered light-duty road vehicles. These vehicles will be for general consumer applications, albeit initially in select markets but with much broader market penetration expected by 2025. To assure international harmony, North American, European, and Asian regulatory representatives are striving to base respective national regulations on an international safety standard, the Global Technical Regulation (GTR), Hydrogen Fueled Vehicle, which is part of an international agreement pertaining to wheeled vehicles and equipment for wheeled vehicles.

  12. Mechanosensory and ATP Release Deficits following Keratin14-Cre-Mediated TRPA1 Deletion Despite Absence of TRPA1 in Murine Keratinocytes.

    Directory of Open Access Journals (Sweden)

    Katherine J Zappia

    Full Text Available Keratinocytes are the first cells that come into direct contact with external tactile stimuli; however, their role in touch transduction in vivo is not clear. The ion channel Transient Receptor Potential Ankyrin 1 (TRPA1 is essential for some mechanically-gated currents in sensory neurons, amplifies mechanical responses after inflammation, and has been reported to be expressed in human and mouse skin. Other reports have not detected Trpa1 mRNA transcripts in human or mouse epidermis. Therefore, we set out to determine whether selective deletion of Trpa1 from keratinocytes would impact mechanosensation. We generated K14Cre-Trpa1fl/fl mice lacking TRPA1 in K14-expressing cells, including keratinocytes. Surprisingly, Trpa1 transcripts were very poorly detected in epidermis of these mice or in controls, and detection was minimal enough to preclude observation of Trpa1 mRNA knockdown in the K14Cre-Trpa1fl/fl mice. Unexpectedly, these K14Cre-Trpa1fl/fl mice nonetheless exhibited a pronounced deficit in mechanosensitivity at the behavioral and primary afferent levels, and decreased mechanically-evoked ATP release from skin. Overall, while these data suggest that the intended targeted deletion of Trpa1 from keratin 14-expressing cells of the epidermis induces functional deficits in mechanotransduction and ATP release, these deficits are in fact likely due to factors other than reduction of Trpa1 expression in adult mouse keratinocytes because they express very little, if any, Trpa1.

  13. Surface profile gradient in amorphous Ta{sub 2}O{sub 5} semi conductive layers regulates nanoscale electric current stability

    Energy Technology Data Exchange (ETDEWEB)

    Cefalas, A.C., E-mail: ccefalas@eie.gr [National Hellenic Research Foundation, Theoretical and Physical Chemistry Institute, 48 Vassileos Constantinou Avenue, Athens 11635 (Greece); Kollia, Z.; Spyropoulos-Antonakakis, N.; Gavriil, V. [National Hellenic Research Foundation, Theoretical and Physical Chemistry Institute, 48 Vassileos Constantinou Avenue, Athens 11635 (Greece); Christofilos, D.; Kourouklis, G. [Physics Division, School of Technology, Aristotle University of Thessaloniki, Thessaloniki 54124 (Greece); Semashko, V.V.; Pavlov, V. [Kazan Federal University, Institute of Physics, 18 Kremljovskaja str., Kazan 420008 (Russian Federation); Sarantopoulou, E. [National Hellenic Research Foundation, Theoretical and Physical Chemistry Institute, 48 Vassileos Constantinou Avenue, Athens 11635 (Greece); Kazan Federal University, Institute of Physics, 18 Kremljovskaja str., Kazan 420008 (Russian Federation)

    2017-02-28

    Highlights: • The work links the surface morphology of amorphous semiconductors with both their electric-thermal properties and current stability at the nanoscale (<1 μm). • Measured high correlation value between surface morphological spatial gradient and conductive electron energy spatial gradient or thermal gradient. • Unidirectional current stability is associated with asymmetric nanodomains along nanosize conductive paths. • Bidirectional current stability is inherent with either long conductive paths or nanosize conductive paths along symmetric nanodomains. • Conclusion: Surface design improves current stability across nanoelectonic junctions. - Abstract: A link between the morphological characteristics and the electric properties of amorphous layers is established by means of atomic, conductive, electrostatic force and thermal scanning microscopy. Using amorphous Ta{sub 2}O{sub 5} (a-Ta{sub 2}O{sub 5}) semiconductive layer, it is found that surface profile gradients (morphological gradient), are highly correlated to both the electron energy gradient of trapped electrons in interactive Coulombic sites and the thermal gradient along conductive paths and thus thermal and electric properties are correlated with surface morphology at the nanoscale. Furthermore, morphological and electron energy gradients along opposite conductive paths of electrons intrinsically impose a current stability anisotropy. For either long conductive paths (L > 1 μm) or along symmetric nanodomains, current stability for both positive and negative currents i is demonstrated. On the contrary, for short conductive paths along non-symmetric nanodomains, the set of independent variables (L, i) is spanned by two current stability/intability loci. One locus specifies a stable state for negative currents, while the other locus also describes a stable state for positive currents.

  14. Electric drives

    CERN Document Server

    Boldea, Ion

    2005-01-01

    ENERGY CONVERSION IN ELECTRIC DRIVESElectric Drives: A DefinitionApplication Range of Electric DrivesEnergy Savings Pay Off RapidlyGlobal Energy Savings Through PEC DrivesMotor/Mechanical Load MatchMotion/Time Profile MatchLoad Dynamics and StabilityMultiquadrant OperationPerformance IndexesProblemsELECTRIC MOTORS FOR DRIVESElectric Drives: A Typical ConfigurationElectric Motors for DrivesDC Brush MotorsConventional AC MotorsPower Electronic Converter Dependent MotorsEnergy Conversion in Electric Motors/GeneratorsPOWER ELECTRONIC CONVERTERS (PECs) FOR DRIVESPower Electronic Switches (PESs)The

  15. Tariff proposal of the Commission of energy regulation from February 28, 2008 for the use of public natural gas distribution networks; Proposition tarifaire de la Commission de regulation de l'energie du 28 fevrier 2008 pour l'utilisation des reseaux publics de distribution de gaz naturel

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    With the complete opening of natural gas markets to competition and the legal separation of distribution networks, Gaz de France Reseau Distribution requested the implementation of a new tariff of use of gas distribution networks to the Commission of energy regulation (CRE). A new tariff of networks utilisation has thus been proposed by CRE after a public consultation and the audition of gas suppliers. This tariff foresees a 5.6% increase of the present day tariff by July 1, 2008. The impact on the end-users' gas retail price will be a 1.5% rise of the regulated tariff. (J.S.)

  16. The use of efficiency improvement factor in regulation of income frame for electric grid companies; Bruk av effektiviseringsfaktor ved inntektsrammeregulering av nettselskap

    Energy Technology Data Exchange (ETDEWEB)

    Andersen, Christian; Singh, Balbir; Storeboe, Inger Oeydis

    2001-06-01

    An efficiency improvement parameter is an integrated part of a family of regulatory mechanisms often denoted as RPI-X. The present report examines what factors are to be included in the efficiency improvement factor. A theoretical derivation shows that the income growth in the regulated sector shall be limited to the price growth of the economy corrected for a factor-X that consists of four components: a productivity component, a price component, a component that accounts for differences in exogenic costs in the regulated sector and the rest of the economy, and a activity level component. The productivity component accounts for the differences in productivity changes between the two sectors, and the price component accounts for the differences in the prices of factor inputs.

  17. Deciphering Cis-Regulatory Element Mediated Combinatorial Regulation in Rice under Blast Infected Condition.

    Science.gov (United States)

    Deb, Arindam; Kundu, Sudip

    2015-01-01

    Combinations of cis-regulatory elements (CREs) present at the promoters facilitate the binding of several transcription factors (TFs), thereby altering the consequent gene expressions. Due to the eminent complexity of the regulatory mechanism, the combinatorics of CRE-mediated transcriptional regulation has been elusive. In this work, we have developed a new methodology that quantifies the co-occurrence tendencies of CREs present in a set of promoter sequences; these co-occurrence scores are filtered in three consecutive steps to test their statistical significance; and the significantly co-occurring CRE pairs are presented as networks. These networks of co-occurring CREs are further transformed to derive higher order of regulatory combinatorics. We have further applied this methodology on the differentially up-regulated gene-sets of rice tissues under fungal (Magnaporthe) infected conditions to demonstrate how it helps to understand the CRE-mediated combinatorial gene regulation. Our analysis includes a wide spectrum of biologically important results. The CRE pairs having a strong tendency to co-occur often exhibit very similar joint distribution patterns at the promoters of rice. We couple the network approach with experimental results of plant gene regulation and defense mechanisms and find evidences of auto and cross regulation among TF families, cross-talk among multiple hormone signaling pathways, similarities and dissimilarities in regulatory combinatorics between different tissues, etc. Our analyses have pointed a highly distributed nature of the combinatorial gene regulation facilitating an efficient alteration in response to fungal attack. All together, our proposed methodology could be an important approach in understanding the combinatorial gene regulation. It can be further applied to unravel the tissue and/or condition specific combinatorial gene regulation in other eukaryotic systems with the availability of annotated genomic sequences and suitable

  18. Deciphering Cis-Regulatory Element Mediated Combinatorial Regulation in Rice under Blast Infected Condition.

    Directory of Open Access Journals (Sweden)

    Arindam Deb

    Full Text Available Combinations of cis-regulatory elements (CREs present at the promoters facilitate the binding of several transcription factors (TFs, thereby altering the consequent gene expressions. Due to the eminent complexity of the regulatory mechanism, the combinatorics of CRE-mediated transcriptional regulation has been elusive. In this work, we have developed a new methodology that quantifies the co-occurrence tendencies of CREs present in a set of promoter sequences; these co-occurrence scores are filtered in three consecutive steps to test their statistical significance; and the significantly co-occurring CRE pairs are presented as networks. These networks of co-occurring CREs are further transformed to derive higher order of regulatory combinatorics. We have further applied this methodology on the differentially up-regulated gene-sets of rice tissues under fungal (Magnaporthe infected conditions to demonstrate how it helps to understand the CRE-mediated combinatorial gene regulation. Our analysis includes a wide spectrum of biologically important results. The CRE pairs having a strong tendency to co-occur often exhibit very similar joint distribution patterns at the promoters of rice. We couple the network approach with experimental results of plant gene regulation and defense mechanisms and find evidences of auto and cross regulation among TF families, cross-talk among multiple hormone signaling pathways, similarities and dissimilarities in regulatory combinatorics between different tissues, etc. Our analyses have pointed a highly distributed nature of the combinatorial gene regulation facilitating an efficient alteration in response to fungal attack. All together, our proposed methodology could be an important approach in understanding the combinatorial gene regulation. It can be further applied to unravel the tissue and/or condition specific combinatorial gene regulation in other eukaryotic systems with the availability of annotated genomic

  19. Use of electrical imaging and distributed temperature sensing methods to characterize surface water-groundwater exchange regulating uranium transport at the Hanford 300 Area, Washington

    Science.gov (United States)

    Slater, Lee D.; Ntarlagiannis, Dimitrios; Day-Lewis, Frederick D.; Mwakanyamale, Kisa; Versteeg, Roelof J.; Ward, Andy; Strickland, Christopher; Johnson, Carole D.; Lane, John W.

    2010-10-01

    We explored the use of continuous waterborne electrical imaging (CWEI), in conjunction with fiber-optic distributed temperature sensor (FO-DTS) monitoring, to improve the conceptual model for uranium transport within the Columbia River corridor at the Hanford 300 Area, Washington. We first inverted resistivity and induced polarization CWEI data sets for distributions of electrical resistivity and polarizability, from which the spatial complexity of the primary hydrogeologic units was reconstructed. Variations in the depth to the interface between the overlying coarse-grained, high-permeability Hanford Formation and the underlying finer-grained, less permeable Ringold Formation, an important contact that limits vertical migration of contaminants, were resolved along ˜3 km of the river corridor centered on the 300 Area. Polarizability images were translated into lithologic images using established relationships between polarizability and surface area normalized to pore volume (Spor). The FO-DTS data recorded along 1.5 km of cable with a 1 m spatial resolution and 5 min sampling interval revealed subreaches showing (1) temperature anomalies (relatively warm in winter and cool in summer) and (2) a strong correlation between temperature and river stage (negative in winter and positive in summer), both indicative of reaches of enhanced surface water-groundwater exchange. The FO-DTS data sets confirm the hydrologic significance of the variability identified in the CWEI and reveal a pattern of highly focused exchange, concentrated at springs where the Hanford Formation is thickest. Our findings illustrate how the combination of CWEI and FO-DTS technologies can characterize surface water-groundwater exchange in a complex, coupled river-aquifer system.

  20. Use of electrical imaging and distributed temperature sensing methods to characterize surface water-groundwater exchange regulating uranium transport at the Hanford 300 Area, Washington

    Science.gov (United States)

    Slater, Lee D.; Ntarlagiannis, Dimitrios; Day-Lewis, Frederick D.; Mwakanyamale, Kisa; Versteeg, Roelof J.; Ward, Andy; Strickland, Christopher; Johnson, Carole D.; Lane, John W.

    2010-01-01

    We explored the use of continuous waterborne electrical imaging (CWEI), in conjunction with fiber-optic distributed temperature sensor (FO-DTS) monitoring, to improve the conceptual model for uranium transport within the Columbia River corridor at the Hanford 300 Area, Washington. We first inverted resistivity and induced polarization CWEI data sets for distributions of electrical resistivity and polarizability, from which the spatial complexity of the primary hydrogeologic units was reconstructed. Variations in the depth to the interface between the overlying coarse-grained, high-permeability Hanford Formation and the underlying finer-grained, less permeable Ringold Formation, an important contact that limits vertical migration of contaminants, were resolved along ∼3 km of the river corridor centered on the 300 Area. Polarizability images were translated into lithologic images using established relationships between polarizability and surface area normalized to pore volume (Spor). The FO-DTS data recorded along 1.5 km of cable with a 1 m spatial resolution and 5 min sampling interval revealed subreaches showing (1) temperature anomalies (relatively warm in winter and cool in summer) and (2) a strong correlation between temperature and river stage (negative in winter and positive in summer), both indicative of reaches of enhanced surface water–groundwater exchange. The FO-DTS data sets confirm the hydrologic significance of the variability identified in the CWEI and reveal a pattern of highly focused exchange, concentrated at springs where the Hanford Formation is thickest. Our findings illustrate how the combination of CWEI and FO-DTS technologies can characterize surface water–groundwater exchange in a complex, coupled river-aquifer system.

  1. The Application of NHEJ-CRISPR/Cas9 and Cre-Lox System in the Generation of Bivalent Duck Enteritis Virus Vaccine against Avian Influenza Virus

    Directory of Open Access Journals (Sweden)

    Pengxiang Chang

    2018-02-01

    Full Text Available Duck-targeted vaccines to protect against avian influenza are critically needed to aid in influenza disease control efforts in regions where ducks are endemic for highly pathogenic avian influenza (HPAI. Duck enteritis virus (DEV is a promising candidate viral vector for development of vaccines targeting ducks, owing to its large genome and narrow host range. The clustered regularly interspaced palindromic repeats (CRISPR/Cas9 system is a versatile gene-editing tool that has proven beneficial for gene modification and construction of recombinant DNA viral vectored vaccines. Currently, there are two commonly used methods for gene insertion: non-homologous end-joining (NHEJ and homology-directed repair (HDR. Owing to its advantages in efficiency and independence from molecular requirements of the homologous arms, we utilized NHEJ-dependent CRISPR/Cas9 to insert the influenza hemagglutinin (HA antigen expression cassette into the DEV genome. The insert was initially tagged with reporter green fluorescence protein (GFP, and a Cre-Lox system was later used to remove the GFP gene insert. Furthermore, a universal donor plasmid system was established by introducing double bait sequences that were independent of the viral genome. In summary, we provide proof of principle for generating recombinant DEV viral vectored vaccines against the influenza virus using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.

  2. Analysis of electricity price in Danish competitive electricity market

    DEFF Research Database (Denmark)

    Hu, Weihao; Chen, Zhe; Bak-Jensen, Birgitte

    2012-01-01

    The Danish power system is currently the grid area in the world that has the largest share of wind power in its generation profiles, with around 20% of its annual consumption generated by wind turbines. In this paper, the Danish power system, which may represent the future of competitive...... electricity markets in some ways, is chosen as the studied power system. 10 year actual data from the Danish competitive electricity market are collected and analyzed. The relationship among the electricity price (both the spot price and the regulation price), the consumption and the wind power generation...... in an electricity market is investigated in this paper. The spot price and the regulation price generally decrease when the wind power penetration in the power system increases or the consumption of the power system decreases. The statistical characteristics of the spot price and the regulation price for different...

  3. Atmospheric electricity

    CERN Document Server

    Chalmers, J Alan

    1957-01-01

    Atmospheric Electricity brings together numerous studies on various aspects of atmospheric electricity. This book is composed of 13 chapters that cover the main problems in the field, including the maintenance of the negative charge on the earth and the origin of the charges in thunderstorms. After a brief overview of the historical developments of atmospheric electricity, this book goes on dealing with the general principles, results, methods, and the MKS system of the field. The succeeding chapters are devoted to some aspects of electricity in the atmosphere, such as the occurrence and d

  4. Electric power industry in China

    Energy Technology Data Exchange (ETDEWEB)

    Zisheng Jiang [Ministry of Electric Power, Beijing (China). Bureau of Electric Power Machinery

    1995-07-01

    This document presents the status of the electric power in China, highlighting the following aspects: recent achievement, electricity increased sharing in the total energy consumption, technical economic indexes, nuclear power, renewable energy sources, rural electrification, transmission and power network, transmission lines and substations, present status and development trends for power network, regulation of power system dispatching, power system communication. The document also presents the future developing plan, approaching the outlook and strategy, development targets of the electric power industry and the administrative system reforming of the electric power industry.

  5. Dynamics of competitive strategies in de-regulated industries: the case of the electricity industry in France; Dynamique des strategies concurrentielles dans un contexte de liberalisation: le cas de l'industrie electrique en France

    Energy Technology Data Exchange (ETDEWEB)

    Cateura, O

    2007-11-15

    This research work is focused on the competitive dynamics approach and rivalry studies between competitors. It develops theses recent perspectives and particularly multi-market competition (also called multipoint competition) in de-regulated industries (network utilities). Indeed, competitive behaviours in liberalized industries are still badly-known. To conduct this research, we decided to analyze a selection of companies (EDF, Electrabel, Endesa, Enel, Gaz de France, Poweo, Direct Energie..) settled on the French electricity market presently in the course of liberalization (1996 - 2006). This qualitative research, through longitudinal case studies, has been developed thanks to a CIFRE agreement (between the French Ministry of Research and the firm Electrabel France) including action research and participant observation. Using multidimensional strategic sequences, we identified two periods, the first one characterized by a confrontation movement and a second one by mutual forbearance. We argue that after learning the rules of a newly liberalized market (confrontation, diversification, internationalization), competitors rapidly and collectively shift there positions towards a focused European strategy based on the gas-electricity convergence. The development of multi-market competition has conducted to mutual forbearance, which was particularly profitable to the major participants. Integrated strategy (market and non-market) appears as an important driver for legitimizing theses behaviours. (author)

  6. REGULATION OF BLOOD PRESSURE, APPETITE AND GLUCOSE BY LEPTIN AFTER INACTIVATION OF INSULIN RECEPTOR SUBSTRATE 2 (IRS2) SIGNALING IN THE ENTIRE BRAIN OR IN POMC NEURONS

    Science.gov (United States)

    do Carmo, Jussara M.; da Silva, Alexandre A.; Wang, Zhen; Freeman, Nathan J.; Alsheik, Ammar J.; Adi, Ahmad; Hall, John E.

    2016-01-01

    Insulin receptor substrate 2 (IRS2) is one of three major leptin receptor signaling pathways, but its role in mediating the chronic effects of leptin on blood pressure, food intake and glucose regulation is unclear. We tested if genetic inactivation of IRS2 in the entire brain (IRS2/Nestin-cre mice) or specifically in proopiomelanocortin (POMC) neurons (IRS2/POMC-cre mice) attenuates the chronic cardiovascular, metabolic and antidiabetic effects of leptin. Mice were instrumented with telemetry probes for measurement of blood pressure (BP) and heart rate (HR) and with venous catheters for intravenous infusions. After a 5-day control period, mice received leptin infusion (2 µg/kg/min) for 7 days. Compared to control IRS2flox/flox mice, IRS2/POMC-cre mice had similar body weight and food intake (33±1 vs. 35±1g and 3.6±0.5 vs. 3.8±0.2 g/day) but higher MAP and HR (110±2 vs. 102±2 mmHg and 641±9 vs. 616±5 bpm). IRS2/Nestin-cre mice were heavier (38±2 g), slightly hyperphagic (4.5±1.0 g/day), and had higher MAP and HR (108±2 mmHg and 659±9 bpm) compared to control mice. Leptin infusion gradually increased MAP despite decreasing food intake by 31% in IRS2flox/flox as well as in Nestin-cre control mice. In contrast, leptin infusion did not change MAP in IRS2/Nestin-cre or IRS2/POMC-cre mice. The anorexic and antidiabetic effects of leptin, however, were similar in all 3 groups. These results indicate that IRS2 signaling in the CNS, and particularly in POMC neurons, is essential for the chronic actions of leptin to raise MAP but not for its anorexic or antidiabetic effects. PMID:26628674

  7. 49 CFR 236.105 - Electric lock.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Electric lock. 236.105 Section 236.105 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION...: All Systems Inspections and Tests; All Systems § 236.105 Electric lock. Electric lock, except forced...

  8. 49 CFR 236.761 - Locking, electric.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Locking, electric. 236.761 Section 236.761 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION... Locking, electric. The combination of one or more electric locks and controlling circuits by means of...

  9. Designing of Loss Optimum Regulator for Control of D.C. Electric Drive with Varying Inertia Moment in CoDeSys Programming System

    Directory of Open Access Journals (Sweden)

    S. O. Novikov

    2009-01-01

    Full Text Available CoDeSys programming system is considered as the most complete version of software for programmed logical controllers (PLC that meets requirements of IEC 61131-3-standard. The given software is the most suitable for simulation and development of control system algorithms and execution of semi-full-scale tests without involvement of an actual object. Programming medium operated at a personal computer in Windows medium. As CoDeSys provides a machine code it is rather easy to support its programming and its minimum support presupposes selection of IN/OUT and program debugging functions.The investigations have been directed on optimization of an electric drive operation with varying inertia moment. The whole software developed for the solution of the considered problem, has been written and realized in the CoDeSys programming system. «Modified principle of maximum» of V. I. Panasiuk is applied as a body of mathematics that allows to obtain positive results of the investigations.

  10. Electric machine

    Science.gov (United States)

    El-Refaie, Ayman Mohamed Fawzi [Niskayuna, NY; Reddy, Patel Bhageerath [Madison, WI

    2012-07-17

    An interior permanent magnet electric machine is disclosed. The interior permanent magnet electric machine comprises a rotor comprising a plurality of radially placed magnets each having a proximal end and a distal end, wherein each magnet comprises a plurality of magnetic segments and at least one magnetic segment towards the distal end comprises a high resistivity magnetic material.

  11. Electric generators: Roesel

    Energy Technology Data Exchange (ETDEWEB)

    Segaser, C.L.

    1977-04-01

    A new and unique type of electrical generator is described that will provide constant frequency output (within 0.01% or better) regardless of rotational speed variations. It accomplishes this with no added bulk over conventional generators, and with excellent efficiency. The same principle that permits constant frequency output also provides the means for output voltage regulation with varying drive speed and/or varying load. Onsite power generation aspects of the Integrated Community Energy Systems (ICES) concept include the requirement to follow community electric loads and the possible use of internal combustion piston engines with waste heat recovery to drive electric generators. The diesel engine is a prime candidate from commercially available technologies for which part-load shaft efficiency is better at reduced speed than for constant-speed operation required by conventional AC generators. The unique characteristics of the Roesel generator allow prime mover speed to decrease with load and offer improved engine-generator system efficiency in ICES applications.

  12. Electrical Injuries

    Science.gov (United States)

    ... it can pass through your body and cause injuries. These electrical injuries can be external or internal. You may have one or both types. External injuries are skin burns. Internal injuries include damage to ...

  13. Electricity derivatives

    CERN Document Server

    Aïd, René

    2015-01-01

    Offering a concise but complete survey of the common features of the microstructure of electricity markets, this book describes the state of the art in the different proposed electricity price models for pricing derivatives and in the numerical methods used to price and hedge the most prominent derivatives in electricity markets, namely power plants and swings. The mathematical content of the book has intentionally been made light in order to concentrate on the main subject matter, avoiding fastidious computations. Wherever possible, the models are illustrated by diagrams. The book should allow prospective researchers in the field of electricity derivatives to focus on the actual difficulties associated with the subject. It should also offer a brief but exhaustive overview of the latest techniques used by financial engineers in energy utilities and energy trading desks.

  14. Near-infrared fluorescent protein iRFP713 as a reporter protein for optogenetic vectors, a transgenic Cre-reporter rat, and other neuronal studies.

    Science.gov (United States)

    Richie, Christopher T; Whitaker, Leslie R; Whitaker, Keith W; Necarsulmer, Julie; Baldwin, Heather A; Zhang, Yajun; Fortuno, Lowella; Hinkle, Josh J; Koivula, Pyry; Henderson, Mark J; Sun, Wenzhi; Wang, Kai; Smith, Jeffrey C; Pickel, Jim; Ji, Na; Hope, Bruce T; Harvey, Brandon K

    2017-06-01

    The use of genetically-encoded fluorescent reporters is essential for the identification and observation of cells that express transgenic modulatory proteins. Near-infrared (NIR) fluorescent proteins have superior light penetration through biological tissue, but are not yet widely adopted. Using the near-infrared fluorescent protein, iRFP713, improves the imaging resolution in thick tissue sections or the intact brain due to the reduced light-scattering at the longer, NIR wavelengths used to image the protein. Additionally, iRFP713 can be used to identify transgenic cells without photobleaching other fluorescent reporters or affecting opsin function. We have generated a set of adeno-associated vectors in which iRFP713 has been fused to optogenetic channels, and can be expressed constitutively or Cre-dependently. iRFP713 is detectable when expressed in neurons both in vitro and in vivo without exogenously supplied chromophore biliverdin. Neuronally-expressed iRFP713 has similar properties to GFP-like fluorescent proteins, including the ability to be translationally fused to channelrhodopsin or halorhodopsin, however, it shows superior photostability compared to EYFP. Furthermore, electrophysiological recordings from iRFP713-labeled cells compared to cells labeled with mCherry suggest that iRFP713 cells are healthier and therefore more stable and reliable in an ex vivo preparation. Lastly, we have generated a transgenic rat that expresses iRFP713 in a Cre-dependent manner. Overall, we have demonstrated that iRFP713 can be used as a reporter in neurons without the use of exogenous biliverdin, with minimal impact on viability and function thereby making it feasible to extend the capabilities for imaging genetically-tagged neurons in slices and in vivo. Published by Elsevier B.V.

  15. The electric motor handbook

    Energy Technology Data Exchange (ETDEWEB)

    Hurst, R.W.; Feltham, P. (eds.)

    2004-05-01

    This handbook outlines the important role that electric motors play in modern society. It covers the field of motor applications from various motor types to their use and repair. It also presents practical applications of electric motors and methods on motor efficiency. More than half of all electricity generated, and 75 per cent of all industrial electricity consumption is consumed by electric motors. Electrical personnel must be aware of all factors involved in electric motors in order to choose and apply the appropriate size of electric motor. These factors include efficiency, sizing and proper application. The efficient use and maximum life expectancy of electric motors depends on proper motor protection, control and maintenance. This handbook includes articles from leading experts on electric motors in modern electrical systems. The content includes: design considerations; proper electric motor sizing techniques; optimal electric motor application; electric motor protection technology; electric motor control principles; electric motor maintenance and troubleshooting; induction electric motors; electric motor bearing currents; electric motor bearing lubrication; electromagnetism; electric motor enclosures; electric motor testing; electric motor repair; DC electric motor; electric motor starters; electric motor brushes; industrial electric motors; electric motor diagrams; AC electric motors; electric motor wiring; electric motor service; electric motor rewinding; electric motor winding; diagram of electric motor wiring; electric motor kit; and, troubleshooting electric motors. A directory of motor manufacturers and suppliers was also included. refs., tabs., figs.

  16. Electricity and gas market observatory. 3. Quarter 2007; Observatoire des marches de l'electricite et du gaz. 3. trimestre 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). Since the 1 of July 2007, all customers can choose their gas and electricity suppliers. The present observatory is including residential customer's statistics. Content: A - The electricity market: The retail electricity market (Introduction, Customer segments and their respective weight, Status at September 30, 2007, Dynamic analysis: 3. Quarter 2007); The wholesale electricity market (Introduction, Wholesale market activity in France, Wholesale market activity in France, Prices on the French wholesale market and European comparison, Import and export volumes, Concentration of the French electricity market); B - The gas market: The retail gas market (Introduction, Customer segments and their respective weight, Status on September 30, 2007, Dynamic analysis: 3. Quarter 2007); The wholesale gas market (Gas pricing and gas markets in Europe, The wholesale market in France); C - Appendices: Electricity and gas market observatories combined glossary, Specific electricity market observatory glossary, Specific gas market observatory glossary.

  17. Electricity and gas market observatory. 1. Quarter 2008; Observatoire des marches de l'electricite et du gaz 1. trimestre 2008

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). Since the 1. of July 2007, all customers can choose their gas and electricity suppliers. Content: A - The electricity market: The retail electricity market (Introduction, Customer segments and their respective weight, Status at March 31, 2007, Dynamic analysis: 1. Quarter 2008); The wholesale electricity market (Introduction, Wholesale market activity in France, Prices on the French wholesale market and European comparison, Import and export volumes, Concentration of the French electricity market); B - The gas market: The retail gas market (Introduction, Customer segments and their respective weight, Status on March 31, 2008, Dynamic analysis: 1. Quarter 2008); The wholesale gas market (Gas pricing and gas markets in Europe, The wholesale market in France, Striking fact of the first quarter 2008); C - Appendices: Electricity and gas market observatories combined glossary, Specific electricity market observatory glossary, Specific gas market observatory glossary.

  18. Electricity and gas market observatory. 2. Quarter 2008; Observatoire des marches de l'electricite et du gaz 2. trimestre 2008

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). Since the 1 of July 2007, all customers can choose their gas and electricity suppliers. Content: A - The electricity market: The retail electricity market (Introduction, Customer segments and their respective weight, Status at June 30, 2008, Dynamic analysis: 2. Quarter 2008); The wholesale electricity market (Introduction, Wholesale market activity in France, Prices on the French wholesale market and European comparison, Import and export volumes, Concentration of the French electricity market); B - The gas market: The retail gas market (Introduction, Customer segments and their respective weight, Status on June 30, 2008, Dynamic analysis: 2. Quarter 2008); The wholesale gas market (Gas pricing and gas markets in Europe, The wholesale market in France); C - Appendices: Electricity and gas market observatories combined glossary, Specific electricity market observatory glossary, Specific gas market observatory glossary.

  19. Electricity and gas market observatory. 3. Quarter 2008; Observatoire des marches de l'electricite et du gaz 3. trimestre 2008

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). Since the 1 of July 2007, all customers can choose their gas and electricity suppliers. Content: A - The electricity market: The retail electricity market (Introduction, Customer segments and their respective weight, Status at September 30, 2008, Dynamic analysis: 3. Quarter 2008); The wholesale electricity market (Introduction, Wholesale market activity in France, Prices on the French wholesale market and European comparison, Import and export volumes, Concentration of the French electricity market); B - The gas market: The retail gas market (Introduction, Customer segments and their respective weight, Status on September 30, 2008, Dynamic analysis: 3. Quarter 2008); The wholesale gas market (Gas pricing and gas markets in Europe, The wholesale market in France); C - Appendices: Electricity and gas market observatories combined glossary, Specific electricity market observatory glossary, Specific gas market observatory glossary.

  20. Electricity and gas market observatory. 4. Quarter 2008; Observatoire des marches de l'electricite et du gaz 4. trimestre 2008

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). Since the 1 of July 2007, all customers can choose their gas and electricity suppliers. Content: A - The electricity market: The retail electricity market (Introduction, Customer segments and their respective weight, Status on December 31, 2008, Dynamic analysis: 4. Quarter 2008); The wholesale electricity market (Introduction, Wholesale market activity in France, Prices on the French wholesale market and European comparison, Import and export volumes, Concentration of the French electricity market); B - The gas market: The retail gas market (Introduction, Customer segments and their respective weight, Status on December 31, 2008, Dynamic analysis: 4. Quarter 2008); The wholesale gas market (Gas pricing and gas markets in Europe, The wholesale market in France, Prices on the French wholesale market and European comparison, Concentration of the French gas market); C - Appendices: Electricity and gas market observatories combined glossary, Specific electricity market observatory glossary, Specific gas market observatory glossary.

  1. Electricity and gas market observatory. 4. Quarter 2007; Observatoire des marches de l'electricite et du gaz 4. trimestre 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    The purpose of the observatory is to provide the general public with indicators for monitoring market deregulation. It both covers the wholesale and retail electricity and gas markets in Metropolitan France. This observatory is updated every three months and data are available on CRE web site (www.cre.fr). Since the 1. of July 2007, all customers can choose their gas and electricity suppliers. Content: A - The electricity market: The retail electricity market (Introduction, Customer segments and their respective weight, Status at December 31, 2007, Dynamic analysis: 4. Quarter 2007); The wholesale electricity market (Introduction, Wholesale market activity in France, Prices on the French wholesale market and European comparison, Import and export volumes, Concentration of the French electricity market, Striking fact of the fourth quarter 2007); B - The gas market: The retail gas market (Introduction, Customer segments and their respective weight, Status on December 31. 2007, Dynamic analysis: 4. Quarter 2007); The wholesale gas market (Gas pricing and gas markets in Europe, The wholesale market in France, Striking fact of the fourth quarter 2007); C - Appendices: Electricity and gas market observatories combined glossary, Specific electricity market observatory glossary, Specific gas market observatory glossary.

  2. Misfit of suprastructures on implants processed by electrical discharge machining or the Cresco method.

    Science.gov (United States)

    Fischer, Jens; Thoma, Andrea; Suter, Ana; Lüthy, Heinz; Luder, Hans-Ulrich; Hämmerle, Christoph Hans-Franz

    2009-06-01

    To assess the accuracy of fit of frameworks on implants processed with electrical discharge machining (EDM) or the Cresco technique (Astra Tech). On 12 identical master casts with implants at positions 9(21), 11(23), and 13(25), high-gold alloy frameworks were produced by standard casting procedure. Six frameworks were used for the Cresco technique (group CRE) by employing specific fixed partial denture supports. The remaining 6 frameworks were cast with prefabricated gold copings and served as control. The finished frameworks were screwed onto implant 25 of the corresponding master cast. Dimensions of the marginal gaps were measured at 4 locations on each implant under the scanning electron microscope, applying the replica technique. Subsequently, the control group was processed by EDM (SAE EDM 2000) (group EDM) and analyzed alike. Statistical analysis of the results was performed with Kruskal-Wallis and Mann-Whitney U tests. The mean marginal gaps were measured as follows (CRE/EDM/control): position 25: 0.0 microm/1.0 +/- 1.6 microm/1.5 +/- 2.1 microm; position 23: 5.2 +/- 5.6 microm/18.7 +/- 29.3 microm/23.6 +/- 30.7 microm; and position 21: 36.0 +/- 21.6 microm/40.7 +/- 31.0 microm/46.0 +/- 41.1 microm. The only statistically significant difference was found at location 23 between group CRE on one side and both group EDM and control on the other side. The strong increase of misfit for group CRE from location 23 to location 21 indicates that laser welding is the crucial parameter in this technique. The Cresco technique has a potential to reduce the marginal gap between implants and suprastructures.

  3. Deciphering the Neuronal Circuitry Controlling Local Blood Flow in the Cerebral Cortex with Optogenetics in PV::Cre Transgenic Mice

    Science.gov (United States)

    Urban, Alan; Rancillac, Armelle; Martinez, Lucie; Rossier, Jean

    2012-01-01

    Although it is know since more than a century that neuronal activity is coupled to blood supply regulation, the underlying pathways remains to be identified. In the brain, neuronal activation triggers a local increase of cerebral blood flow (CBF) that is controlled by the neurogliovascular unit composed of terminals of neurons, astrocytes, and blood vessel muscles. It is generally accepted that the regulation of the neurogliovascular unit is adjusted to local metabolic demand by local circuits. Today experimental data led us to realize that the regulatory mechanisms are more complex and that a neuronal system within the brain is devoted to the control of local brain-blood flow. Recent optogenetic experiments combined with functional magnetic resonance imaging have revealed that light stimulation of neurons expressing the calcium binding protein parvalbumin (PV) is associated with positive blood oxygen level-dependent (BOLD) signal in the corresponding barrel field but also with negative BOLD in the surrounding deeper area. Here, we demonstrate that in acute brain slices, channelrhodopsin-2 (ChR2) based photostimulation of PV containing neurons gives rise to an effective contraction of penetrating arterioles. These results support the neurogenic hypothesis of a complex distributed nervous system controlling the CBF. PMID:22715327

  4. Role of proopiomelanocortin neuron Stat3 in regulating arterial pressure and mediating the chronic effects of leptin.

    Science.gov (United States)

    Dubinion, John H; do Carmo, Jussara M; Adi, Ahmad; Hamza, Shereen; da Silva, Alexandre A; Hall, John E

    2013-05-01

    Although signal transducer and activator of transcription 3 (Stat3) is a key second messenger by which leptin regulates appetite and body weight, its role in specific neuronal populations in metabolic regulation and in mediating the chronic effects of leptin on blood pressure is unknown. The current study tested the hypothesis that Stat3 signaling in proopiomelanocortin (POMC) neurons mediates the chronic effects of leptin on mean arterial pressure (MAP), as well as on glucose regulation, energy expenditure, and food intake. Stat3(flox/flox) mice were crossed with POMC-Cre mice to generate mice with Stat3 deletion specifically in POMC neurons (Stat3(flox/flox)/POMC-Cre). Oxygen consumption (Vo2), carbon dioxide respiration (Vco2), motor activity, heat production, food intake, and MAP were measured 24 hours/d. After baseline measurements, leptin was infused (4 μg/kg per min, IP) for 7 days. Stat3(flox/flox)/POMC-Cre mice were hyperphagic, heavier, and had increased respiratory quotients compared with control Stat3(flox/flox) mice. Baseline MAP was not different between the groups, and chronic leptin infusion reduced food intake similarly in both groups (27 versus 29%). Vo2, Vco2, and heat production responses to leptin were not significantly different in control and Stat3(flox/flox)/POMC-Cre mice. However, leptin-mediated increases in MAP were completely abolished, and blood pressure responses to acute air-jet stress were attenuated in male Stat3(flox/flox)/POMC-Cre mice. These results indicate that Stat3 signaling in POMC neurons is essential for leptin-mediated increases in MAP, but not for anorexic or thermogenic effects of leptin.

  5. Role of Shp2 in forebrain neurons in regulating metabolic and cardiovascular functions and responses to leptin

    Science.gov (United States)

    do Carmo, Jussara M.; da Silva, Alexandre A.; Sessums, Price O.; Ebaady, Sabira H.; Pace, Benjamin R.; Rushing, John S.; Davis, Mark T.; Hall, John E.

    2014-01-01

    Objective We examined whether deficiency of Shp2 signaling in forebrain neurons alters metabolic and cardiovascular regulation under various conditions and if it attenuates the anorexic and cardiovascular effects of leptin. We also tested whether forebrain Shp2 deficiency alters blood pressure (BP) and heart rate (HR) responses to acute stress. Design Forebrain Shp2-/- mice were generated by crossing Shp2flox/flox mice with CamKIIα-cre mice. At 22 to 24 weeks of age, mice were instrumented for telemetry for measurement of BP, HR and body temperature (BT). Oxygen consumption (VO2), energy expenditure and motor activity were monitored by indirect calorimetry. Results Shp2/CamKIIα-cre mice were heavier (46±3 vs 32±1 g), hyperglycemic, hyperleptinemic, hyperinsulinemic, and hyperphagic compared to Shp2flox/flox control mice. Shp2/CamKIIα-cre mice exhibited reduced food intake responses to fasting/refeeding and impaired regulation of BT when exposed to 15°C and 30°C ambient temperatures. Despite being obese and having many features of metabolic syndrome, Shp2/CamKIIα-cre mice had similar daily average BP and HR compared to Shp2flox/flox mice (112±2 vs 113±1 mmHg and 595±34 vs 650±40 bpm), but exhibited increased BP and HR responses to cold exposure and acute air-jet stress test. Leptin's ability to reduce food intake and to raise BP were markedly attenuated in Shp2/CamKIIα-cre mice. Conclusion These results suggest that forebrain Shp2 signaling regulates food intake, appetite responses to caloric deprivation, and thermogenic control of body temperature during variations in ambient temperature. Deficiency of Shp2 signaling in the forebrain is associated with augmented cardiovascular responses to cold and acute stress but attenuated BP responses to leptin. PMID:24030516

  6. The ERK1/2 pathway regulates testosterone synthesis by coordinately regulating the expression of steroidogenic genes in Leydig cells.

    Science.gov (United States)

    Matzkin, Maria Eugenia; Yamashita, Soichi; Ascoli, Mario

    2013-05-06

    Adult mice with a Leydig cell specific deletion of MAPK kinase (MEK) 1 and 2 (Mek1(f)(/)(f);Mek2(-/-);Cre(+)) mice display Leydig cell hypoplasia and hypergonadotropic hypogonadism. We used radioimmunoassays and quantitative PCR to evaluate the function and expression of the Leydig cell genes involved in the conversion of cholesterol to testosterone (Star, Cyp11a1, Hsd3b6, Cyp17a1 and Hsd17b3), androgen metabolism (Srda1 and Dhrs9), and four transcription factors (Creb1, Nr5a1, Nr4a1 and Nr0b1) that regulate the expression of steroidogenic genes. We show that Star, Hsd3b6, Cyp17a1 and Hsd17b3 are downregulated in Ledyig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice whereas Srda1 and Dhrs9 are upregulated and Creb1, Nr5a1, Nr4a1 and Nr0b1 are unchanged or upregulated. Functionally, all the downregulated genes but none of the upregulated genes contribute to the decrease in testosterone synthesis in Leydig cells of adult Mek1(f)(/)(f);Mek2(-/-);Cre(+) mice because they produce low testosterone and dihydrotestosterone when stimulated with hCG or when incubated with testosterone precursors such as progesterone or androstenedione. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  7. Ephemeral Electric Potential and Electric Field Sensor

    Science.gov (United States)

    Generazio, Edward R. (Inventor)

    2017-01-01

    Systems, methods, and devices of the various embodiments provide for the minimization of the effects of intrinsic and extrinsic leakage electrical currents enabling true measurements of electric potentials and electric fields. In an embodiment, an ephemeral electric potential and electric field sensor system may have at least one electric field sensor and a rotator coupled to the electric field sensor and be configured to rotate the electric field sensor at a quasi-static frequency. In an embodiment, ephemeral electric potential and electric field measurements may be taken by rotating at least one electric field sensor at a quasi-static frequency, receiving electrical potential measurements from the electric field sensor when the electric field sensor is rotating at the quasi-static frequency, and generating and outputting images based at least in part on the received electrical potential measurements.

  8. RESTRUCTURED ELECTRICITY MARKETS: Three States' Experiences in Adding Generating Capacity

    National Research Council Canada - National Science Library

    2002-01-01

    ...., restructured electricity markets by shifting from service provided through a regulated monopoly-the local electric utility-to service provided through open competition among the local utility and its competitors...

  9. Report made on behalf of the commission of economic affairs, of environment and of the territory about the proposal of law, adopted by the Senate, relative to electricity and natural gas regulated tariffs; Rapport fait au nom de la Commission des Affaires Economiques, de l'Environnement et du Territoire sur la proposition de loi, adoptee par le Senat, relative aux tarifs reglementes d'electricite et de gaz naturel (n.238)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-12-15

    This report aims at filling up some gaps in the French legislation relative to the determination of electricity and natural gas regulated tariffs and to the beneficiaries of these tariffs. It makes a synthesis of four proposals of law dealing with: the possibility for end-users and small companies to benefit again of the regulated electricity prices (proposals no 369 and 149), the permission of reversibility in the exercise of the rights relative to the eligibility for the purchase of electric power (proposal no 427), and the preservation of households' purchasing power by keeping up the regulated prices of electricity and natural gas (proposal no 462). The different articles are examined and some amendments are proposed by the commission. (J.S.)

  10. Electric fields and electrical insulation

    DEFF Research Database (Denmark)

    McAllister, Iain Wilson

    2002-01-01

    The adoption of a field-theoretical approach to problems arising in the framework of electrical insulation is discussed with reference to six main topics, which have been addressed over the last 30 years. These include uniform field electrodes, Green's differential equation, electrode surface...... roughness, induced charge, electrostatic probes, and partial discharge transients, together with several follow-on aspects. Each topic is introduced and thereafter the progress achieved through the use of a field-theoretical approach is reviewed. Because the topics cover a wide spectrum of conditions......, it is amply demonstrated that such an approach can lead to significant progress in many areas of electrical insulation....

  11. Disparities and Trends in Door-to-Needle Time: The FL-PR CReSD Study (Florida-Puerto Rico Collaboration to Reduce Stroke Disparities).

    Science.gov (United States)

    Oluwole, Sofia A; Wang, Kefeng; Dong, Chuanhui; Ciliberti-Vargas, Maria A; Gutierrez, Carolina M; Yi, Li; Romano, Jose G; Perez, Enmanuel; Tyson, Brittany Ann; Ayodele, Maranatha; Asdaghi, Negar; Gardener, Hannah; Rose, David Z; Garcia, Enid J; Zevallos, Juan Carlos; Foster, Dianne; Robichaux, Mary; Waddy, Salina P; Sacco, Ralph L; Rundek, Tatjana

    2017-08-01

    In the United States, about half of acute ischemic stroke patients treated with tPA (tissue-type plasminogen activator) receive treatment within 60 minutes of hospital arrival. We aimed to determine the proportion of patients receiving tPA within 60 minutes (door-to-needle time [DTNT] ≤60) and 45 minutes (DTNT ≤45) of hospital arrival by race/ethnicity and sex and to identify temporal trends in DTNT ≤60 and DTNT ≤45. Among 65 654 acute ischemic stroke admissions in the National Institute of Neurological Disorders and Stroke-funded FL-PR CReSD study (Florida-Puerto Rico Collaboration to Reduce Stroke Disparities) from 2010 to 2015, we included 6181 intravenous tPA-treated cases (9.4%). Generalized estimating equations were used to determine predictors of DTNT ≤60 and DTNT ≤45. DTNT ≤60 was achieved in 42% and DTNT ≤45 in 18% of cases. After adjustment, women less likely received DTNT ≤60 (odds ratio, 0.81; 95% confidence interval, 0.72-0.92) and DTNT ≤45 (odds ratio, 0.73; 95% confidence interval, 0.57-0.93). Compared with Whites, Blacks less likely had DTNT ≤45 during off hours (odds ratio, 0.68; 95% confidence interval, 0.47-0.98). Achievement of DTNT ≤60 and DTNT ≤45 was highest in South Florida (50%, 23%) and lowest in West Central Florida (28%, 11%). In the FL-PR CReSD, achievement of DTNT ≤60 and DTNT ≤45 remains low. Compared with Whites, Blacks less likely receive tPA treatment within 45 minutes during off hours. Treatment within 60 and 45 minutes is lower in women compared with men and lowest in West Central Florida compared with other Florida regions and Puerto Rico. Further research is needed to identify reasons for delayed thrombolytic treatment in women and Blacks and factors contributing to regional disparities in DTNT. © 2017 American Heart Association, Inc.

  12. Deregulation of Electricity Market and Drivers of Demand for Electrical Energy in Industry

    Directory of Open Access Journals (Sweden)

    Bojnec Štefan

    2016-09-01

    Full Text Available This paper investigates deregulation of electricity market focusing on electricity prices and drivers of demand for electrical energy in industry in Slovenia. The patterns in evolution of real electricity price developments and the three main components of the electricity price are calculated: liberalized market share for purchased electricity price, regulated infrastructure share for use of electricity network grids and mandatory state charges in the sale of electricity (duty, excise duty and value-added tax. To calculate the real value of electricity prices, producer price index of industrial commodities for electricity prices in industry is used as deflator and implicit deflator of gross domestic product for the size of the economy. In the empirical econometric part is used regression analysis for the amount electricity consumption in the industry depending on the real gross domestic product, direct and cross-price elasticity for natural gas prices in the industry. The results confirmed volatility in real electricity price developments with their increasing tendency and the increasing share of different taxes and state charges in the electricity prices for industry. Demand for electrical energy in industry is positively associated with gross domestic product and price of natural gas as substitute for electrical energy in industry use, and negatively associated with prices of electrical energy for industry.

  13. Synergistic and Dose-Controlled Regulation of Cellulase Gene Expression in Penicillium oxalicum

    Science.gov (United States)

    Li, Zhonghai; Yao, Guangshan; Wu, Ruimei; Gao, Liwei; Kan, Qinbiao; Liu, Meng; Yang, Piao; Liu, Guodong; Qin, Yuqi; Song, Xin; Zhong, Yaohua; Fang, Xu; Qu, Yinbo

    2015-01-01

    Filamentous fungus Penicillium oxalicum produces diverse lignocellulolytic enzymes, which are regulated by the combinations of many transcription factors. Here, a single-gene disruptant library for 470 transcription factors was constructed and systematically screened for cellulase production. Twenty transcription factors (including ClrB, CreA, XlnR, Ace1, AmyR, and 15 unknown proteins) were identified to play putative roles in the activation or repression of cellulase synthesis. Most of these regulators have not been characterized in any fungi before. We identified the ClrB, CreA, XlnR, and AmyR transcription factors as critical dose-dependent regulators of cellulase expression, the core regulons of which were identified by analyzing several transcriptomes and/or secretomes. Synergistic and additive modes of combinatorial control of each cellulase gene by these regulatory factors were achieved, and cellulase expression was fine-tuned in a proper and controlled manner. With one of these targets, the expression of the major intracellular β-glucosidase Bgl2 was found to be dependent on ClrB. The Bgl2-deficient background resulted in a substantial gene activation by ClrB and proved to be closely correlated with the relief of repression mediated by CreA and AmyR during cellulase induction. Our results also signify that probing the synergistic and dose-controlled regulation mechanisms of cellulolytic regulators and using it for reconstruction of expression regulation network (RERN) may be a promising strategy for cellulolytic fungi to develop enzyme hyper-producers. Based on our data, ClrB was identified as focal point for the synergistic activation regulation of cellulase expression by integrating cellulolytic regulators and their target genes, which refined our understanding of transcriptional-regulatory network as a “seesaw model” in which the coordinated regulation of cellulolytic genes is established by counteracting activators and repressors. PMID:26360497

  14. Disruption of SATB2 or its long-range cis-regulation by SOX9 causes a syndromic form of Pierre Robin sequence.

    Science.gov (United States)

    Rainger, Jacqueline K; Bhatia, Shipra; Bengani, Hemant; Gautier, Philippe; Rainger, Joe; Pearson, Matt; Ansari, Morad; Crow, Jayne; Mehendale, Felicity; Palinkasova, Bozena; Dixon, Michael J; Thompson, Pamela J; Matarin, Mar; Sisodiya, Sanjay M; Kleinjan, Dirk A; Fitzpatrick, David R

    2014-05-15

    Heterozygous loss-of-function (LOF) mutations in the gene encoding the DNA-binding protein, SATB2, result in micrognathia and cleft palate in both humans and mice. In three unrelated individuals, we show that translocation breakpoints (BPs) up to 896 kb 3' of SATB2 polyadenylation site cause a phenotype which is indistinguishable from that caused by SATB2 LOF mutations. This syndrome comprises long nose, small mouth, micrognathia, cleft palate, arachnodactyly and intellectual disability. These BPs map to a gene desert between PLCL1 and SATB2. We identified three putative cis-regulatory elements (CRE1-3) using a comparative genomic approach each of which would be placed in trans relative to SATB2 by all three BPs. CRE1-3 each bind p300 and mono-methylated H3K4 consistent with enhancer function. In silico analysis suggested that CRE1-3 contain one or more conserved SOX9-binding sites, and this binding was confirmed using chromatin immunoprecipitation on cells derived from mouse embryonic pharyngeal arch. Interphase bacterial artificial chromosome fluorescence in situ hybridization measurements in embryonic craniofacial tissues showed that the orthologous region in mice exhibits Satb2 expression-dependent chromatin decondensation consistent with Satb2 being a target gene of CRE1-3. To assess their in vivo function, we made multiple stable reporter transgenic lines for each enhancer in zebrafish. CRE2 was shown to drive SATB2-like expression in the embryonic craniofacial region. This expression could be eliminated by mutating the SOX9-binding site of CRE2. These observations suggest that SATB2 and SOX9 may be acting together via complex cis-regulation to coordinate the growth of the developing jaw.

  15. La regulació de l’administració electrònica i els seus principis rectors - The Regulation of the Electronic Administration and its Guiding Principles - La regulación de la Administración electrónica y sus principios rectores

    Directory of Open Access Journals (Sweden)

    Irene Araguàs Galcerà

    2012-12-01

    paper” government to an electronic government has entailed a series of reforms of our legal system: new regulations, the reform of existing regulations governing the technical aspects derived from the use of new technologies by government authorities, and the rights and guarantees that inure to citizens when they contact government authorities telematically. With this background, we can define a cast of principles governing the behavior of an electronic administration. The leading roles will fall to those principles that aim to guarantee equality while prohibiting discrimination against the citizenry, thereby combating what has come to be known as the “digital divide.”Actualmente, nos encontramos inmersos en un proceso de globalización y tecnificación al que las Administraciones públicas, como parte integrante de la sociedad, no pueden permanecer ajenas. Todas las Administraciones, en sus distintos niveles y ámbitos de actuación, han introducido en su actividad las nuevas tecnologías de la información y la comunicación, y han puesto en marcha un proceso transformación de carácter cultural, organizativo y jurídico que ha fructificado en un nuevo modelo de gestión de la actividad pública conocido bajo el nombre de Administración electrónica.El uso de las nuevas tecnologías y la transición de una Administración «en papel» a una Administración electrónica han conllevado una serie de reformas de nuestro ordenamiento jurídico: nuevas normas y reforma de otras ya existentes que regulan los aspectos técnicos derivados de la utilización de las nuevas tecnologías por parte de la Administración, así como los derechos y garantías de los que están revestidos los ciudadanos cuando se relacionan con la Administración por medios telemáticos. Con este telón de fondo, podemos definir un elenco de principios que regirán la actuación de la Administración electrónica y en los que se erigen como protagonistas aquellos que persiguen garantizar la igualdad y la no

  16. Electricity unplugged

    Science.gov (United States)

    Karalis, Aristeidis

    2009-02-01

    The judge was driving back late one cold winter night. Entering the garage, the battery-charging indicator in his wirelessly powered electric car came on. "Home at last," crossed his mind. He swiped his personal smartcard on the front-door detector to be let in. He heard a "charging" beep from his mobile phone. The blinking cursor on the half-finished e-mail on the laptop had been waiting all day on the side table. He picked the computer up and walked towards his desk. "Good evening, your honour. Your wirelessly heated robe," said the butler-robot as it approached from the kitchen. Putting on the electric garment, he sat on the medical desk chair. His artificial heart was now beating faster.

  17. Electric Car

    Science.gov (United States)

    1977-01-01

    NASA's Lewis Research Center undertook research toward a practical, economical battery with higher energy density. Borrowing from space satellite battery technology, Lewis came up with a nickel-zinc battery that promises longer life and twice the range of the lead-acid counterpart. Lewis researchers fabricated a prototype battery and installed it in an Otis P-500 electric utility van, using only the battery space already available and allowing battery weight equal to that of the va's conventional lead-acid battery

  18. La credibilidad de los medios de Comunicación de masas: una aproximación desde el Modelo de Marca Creíble

    Directory of Open Access Journals (Sweden)

    Cristina Calvo-Porral

    Full Text Available Este estudio tiene por objetivo investigar, en el entorno actual de los medios de Comunicación, la relación entre el valor de marca de los medios de Comunicación desde el enfoque del consumidor y su credibilidad, aplicando el Modelo de Marca Creíble para analizar cómo la audiencia elabora un juicio sobre la credibilidad de los medios. Los datos recogidos mediante un cuestionario estructurado sobre las percepciones de los consumidores, se analizaron mediante un modelo de ecuaciones estructurales. Nuestros resultados sugieren la existencia de una relación positiva entre el valor de marca de los medios de Comunicación y su credibilidad, mostrando que tanto la imagen o asociaciones, juntamente com la lealtad, ejercen la mayor influencia sobre el valor de los medios de Comunicación. Por tanto, la credibilidad de los medios de Comunicación implica una mejora de los procesos y de la propia imagen de éstos, así como el desarrollo de nuevas formas de tratamiento de las noticias y de las necesidades de información de la audiencia.

  19. Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice.

    Directory of Open Access Journals (Sweden)

    Lukas E Dow

    Full Text Available Tetracycline or doxycycline (dox-regulated control of genetic elements allows inducible, reversible and tissue specific regulation of gene expression in mice. This approach provides a means to investigate protein function in specific cell lineages and at defined periods of development and disease. Efficient and stable regulation of cDNAs or non-coding elements (e.g. shRNAs downstream of the tetracycline-regulated element (TRE requires the robust expression of a tet-transactivator protein, commonly the reverse tet-transactivator, rtTA. Most rtTA strains rely on tissue specific promoters that often do not provide sufficient rtTA levels for optimal inducible expression. Here we describe the generation of two mouse strains that enable Cre-dependent, robust expression of rtTA3, providing tissue-restricted and consistent induction of TRE-controlled transgenes. We show that these transgenic strains can be effectively combined with established mouse models of disease, including both Cre/LoxP-based approaches and non Cre-dependent disease models. The integration of these new tools with established mouse models promises the development of more flexible genetic systems to uncover the mechanisms of development and disease pathogenesis.

  20. Solar Electricity

    Science.gov (United States)

    1988-01-01

    ARCO Solar manufactures PV Systems tailored to a broad variety of applications. PV arrays are routinely used at remote communications installations to operate large microwave repeaters, TV and radio repeaters rural telephone, and small telemetry systems that monitor environmental conditions. Also used to power agricultural water pumping systems, to provide electricity for isolated villages and medical clinics, for corrosion protection for pipelines and bridges, to power railroad signals, air/sea navigational aids, and for many types of military systems. ARCO is now moving into large scale generation for utilities.

  1. Risk and investment management in liberalized electricity markets

    DEFF Research Database (Denmark)

    Lemming, Jacob Kjærgaard

    2005-01-01

    are relatively broad, however the two chapters and ve papers all share analyzes of nancial risk in liberalized electricity markets as a common underlying theme. The risk management part of the thesis focusses on modelling and measurement of financial risk in electricity markets. Key topics are electricity price......Electricity markets around the world are currently undergoing a liberalization process that changes the way electricity is traded and priced as a commodity. The electricity system has unique technical characteristics and the importance of electricity as a good in today's informational society...... will be held financial responsible for their decisions. Regulated system operators remain responsibility for the physical balancing and electricity markets will therefore remain strongly regulated even after liberalization. The combination of strongly regulated but competitive trading arrangements creates...

  2. Climate Action Benefits: Electricity

    Science.gov (United States)

    This page provides background on the relationship between electricity and climate change and describes what the CIRA Electricity analyses cover. It provides links to the subsectors Electricity Demand and Electricity Supply.

  3. Electrical Signaling, Photosynthesis and Systemic Acquired Acclimation

    Directory of Open Access Journals (Sweden)

    Magdalena Szechyńska-Hebda

    2017-09-01

    Full Text Available Electrical signaling in higher plants is required for the appropriate intracellular and intercellular communication, stress responses, growth and development. In this review, we have focus on recent findings regarding the electrical signaling, as a major regulator of the systemic acquired acclimation (SAA and the systemic acquired resistance (SAR. The electric signaling on its own cannot confer the required specificity of information to trigger SAA and SAR, therefore, we have also discussed a number of other mechanisms and signaling systems that can operate in combination with electric signaling. We have emphasized the interrelation between ionic mechanism of electrical activity and regulation of photosynthesis, which is intrinsic to a proper induction of SAA and SAR. In a special way, we have summarized the role of non-photochemical quenching and its regulator PsbS. Further, redox status of the cell, calcium and hydraulic waves, hormonal circuits and stomatal aperture regulation have been considered as components of the signaling. Finally, a model of light-dependent mechanisms of electrical signaling propagation has been presented together with the systemic regulation of light-responsive genes encoding both, ion channels and proteins involved in regulation of their activity. Due to space limitations, we have not addressed many other important aspects of hormonal and ROS signaling, which were presented in a number of recent excellent reviews.

  4. Regulating the regulators : accountability of Australian regulators

    National Research Council Canada - National Science Library

    Bird, Joanna

    2011-01-01

    Accountability of Australian regulators - Australian Securities and Investments Commission - Australian Prudential Regulation Authority - concept of 'accountability' - mechanisms for accountability...

  5. Topics on electricity trade

    Energy Technology Data Exchange (ETDEWEB)

    Skytte, K.

    2001-07-01

    The first chapter in this thesis is a survey of studies on electricity trade, which has been included in order to show how subjects in this thesis fit into this aspect of energy research. Transitions from centralised monopolies to liberalised electricity markets have been inaugurated in the northern European countries during the last decade. The backgrounds and speed of transitions differ within the countries. Where Norway liberalised its electricity market in order to stabilise the prices, EU urged a liberalisation in order to make the electricity supply industry more effective. Together with national liberalisation, the opening of the cross border trade between the countries is also urged. The first paper in this thesis describes those different liberalisation processes within northern Europe and discusses problems that may arise in a transition period for integrating the countries into a common electricity market with efficient cross-border competition. Before the liberalisation process started, the electricity generation was determined by a central load dispatch at known prices. The introduction of competition has necessitated the creation of new competitive markets, power exchanges and financial markets where generation and prices are determined by demand and supply on the markets. One implication of this is that investments in new plants shall be made under levels of uncertainty about future prices. The second paper in this thesis analyses the importance of introducing uncertainty in energy-economic modelling of the electricity supply sector. Another implication, which arises on introducing a new market structure and power exchanges, is that the design of the power exchanges influences the price setting on the markets. The third paper of this thesis analyses the price setting on the regulating power market on the Nordic power exchange Nord Pool. More precisely, the paper reveals the pattern of the prices on the regulating power market by analysing the cost of

  6. UK electricity `94

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-12-31

    A detailed factual account is presented of the achievements of the UK electricity industry in 1994. The review is divided into sections headed: the UK energy market and electricity`s share; the electricity market; electricity prices; the electric power supply system; quality of service; protection of the environment; manpower and safety trends; business diverisification and the electricity industry in the European Union. Statistical tables are presented on power stations in the UK and key electricity and energy statistics.

  7. Cre-/IoxP-Mediated Recombination between the SIL and SCL Genes Leads to a Block in T-Cell Development at the CD4-CD8- to CD4+CD8+ Transition

    Directory of Open Access Journals (Sweden)

    Yue Cheng

    2007-04-01

    Full Text Available In the most common form of stem cell leukemia (SCL gene rearrangement, an interstitial deletion of 82 kb brings SCL under the control of regulatory elements that normally govern expression of the ubiquitously expressed SCL interrupting locus (SIL gene, which is located directly upstream of SCL. To investigate the effect of this fusion in a mouse model, a bacterial artificial chromosome (BAC clone containing both human SIL and SCL genes was isolated, and IoxP sites were inserted into intron 1 of both the SIL and SCL genes, corresponding to the sites at which recombination occurs in human T-cell acute lymphocytic leukemia patients. This BAC clone was used to generate transgenic SILIoxloxSCL mice. These transgenic mice were subsequently bred to Lck-Cre mice that express the Cre recombinase specifically in the thymus. The BAC transgene was recombined between the two IoxP sites in over 50% of the thymocytes from SILIoxloxSCL/Cre double-transgenic mice, bringing the SCL gene under the direct control of SIL regulatory elements. Aberrant SCL gene expression in the thymus was verified by reverse transcription- polymerase chain reaction. Using FACS analysis, we found that mice carrying both SILIoxloxSCL and Cre transgenes have increased CD4-/CD8- thymocytes compared with transgenenegative mice. In the spleen, these transgenic mice show a marked reduction in the number of mature CD4+ or CD8+ cells. These results demonstrate that conditional activation of SCL under control of SIL regulatory elements can impair normal T-cell development.

  8. Restructuring nuclear regulations.

    OpenAIRE

    Mossman, Kenneth L

    2003-01-01

    Nuclear regulations are a subset of social regulations (laws to control activities that may negatively impact the environment, health, and safety) that concern control of ionizing radiation from radiation-producing equipment and from radioactive materials. The impressive safety record among nuclear technologies is due, in no small part, to the work of radiation safety professionals and to a protection system that has kept pace with the rapid technologic advancements in electric power generati...

  9. Electric industry restructuring review

    Energy Technology Data Exchange (ETDEWEB)

    Slocum Hollis, S. [Duane Morris LLP, Washington, DC (United States)

    2004-07-01

    Restructuring of the electric power industry began in the early 1990's in many jurisdictions in the United States. Restructuring was an attempt to offer large industrial customers lower rates and freedom from regulation for generators and traditional public utilities. The move has gained most attention in the past two years as some utilities report high profits while others, such as Pacific Gas and Electric Co., the largest investor-owned utility in the United States, is in bankruptcy. The August 2003 blackout in the Midwest and Northeast United States and Canada also raised questions regarding electric reliability. The question now remains whether markets should be allowed to determine the need for services and the prices to be charged, and who is in charge in the imperfect market. The Federal Energy Regulatory Commission's (FERC) Order 2000 led to the formation of a Regional Transmission Organizations which is still in the implementation stage. Its influence on precursor Order numbers 888 and 889 were discussed in this paper with reference to independent system operators; regional transmission organizations; standard market design; rates and pricing devices; congestion management; market monitoring; market investigations; reliability measures; OASIS and other information access; interconnection policy; jurisdiction; mergers and merger policy; standards of conduct; policing affiliates; municipal utilities; stranded costs; and, state restructuring scorecards. refs.

  10. Electric-Drive Vehicles

    Energy Technology Data Exchange (ETDEWEB)

    Septon, Kendall K [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-09-11

    Electric-drive vehicles use electricity as their primary fuel or to improve the efficiency of conventional vehicle designs. These vehicles can be divided into three categories: Hybrid electric vehicles (HEVs), Plug-in hybrid electric vehicles (PHEVs), All-electric vehicles (EVs). Together, PHEVs and EVs can also be referred to as plug-in electric vehicles (PEVs).

  11. Basic Electricity. Part 2.

    Science.gov (United States)

    Kilmer, Donald C.

    This guide, the second (part 2) in a set of four guides, is designed for the student interested in a vocation in electrical work, and includes two units: Unit IV--Electrical Theory, covering thirteen lessons (matter, the atom, electrical charges in the atom, rules of electric charges, electricity, atoms in an electrical conductor, electrical…

  12. Electric-Drive Vehicles

    Energy Technology Data Exchange (ETDEWEB)

    None

    2017-09-01

    Electric-drive vehicles use electricity as their primary fuel or to improve the efficiency of conventional vehicle designs. These vehicles can be divided into three categories: Hybrid electric vehicles (HEVs), Plug-in hybrid electric vehicles (PHEVs), All-electric vehicles (EVs). Together, PHEVs and EVs can also be referred to as plug-in electric vehicles (PEVs).

  13. HMGA1 expression levels are elevated in pancreatic intraepithelial neoplasia cells in the Ptf1a-Cre; LSL-KrasG12D transgenic mouse model of pancreatic cancer.

    Science.gov (United States)

    Veite-Schmahl, Michelle J; Joesten, William C; Kennedy, Michael A

    2017-08-22

    Pancreatic cancer is currently the third leading cause of cancer deaths in the United States and it is predicted to become the second by the year 2030. High-mobility group A1 protein (HMGA1) is an oncogenic transcription factor, localised and active in cell nuclei, that is linked to tumour progression in many human cancers, including pancreatic cancer. Overexpression of HMGA1 renders cancer cells resistant to chemotherapy. Although the Ptf1a-Cre; LSL-KrasG12D transgenic mouse is perhaps the most widely utilised animal model for human pancreatic cancer, expression levels of HMGA1 in pancreata from this mouse model have not been characterised. Quantitative immunohistochemical analysis was used to determine nuclear HMGA1 levels in pancreatic tissue sections from Ptf1a-Cre; LSL-KrasG12D mice aged 5, 11, and 15 months. The H Score method was used for quantitative analysis. The HMGA1 levels were significantly elevated in pancreatic intraepithelial neoplasia (PanIN) epithelia compared with untransformed acinar tissues or fibroinflammatory stroma. The PanINs have long been regarded as precancerous precursors to pancreatic adenocarcinoma. Significantly elevated HMGA1 levels observed in the nuclei of PanINs in Ptf1a-Cre; LSL-KrasG12D mice validate this animal model for investigating the role that HMGA1 plays in cancer progression and testing therapeutic approaches targeting HMGA1 in human cancers.

  14. TCreERT2, a transgenic mouse line for temporal control of Cre-mediated recombination in lineages emerging from the primitive streak or tail bud.

    Science.gov (United States)

    Anderson, Matthew J; Naiche, L A; Wilson, Catherine P; Elder, Cindy; Swing, Deborah A; Lewandoski, Mark

    2013-01-01

    The study of axis extension and somitogenesis has been greatly advanced through the use of genetic tools such as the TCre mouse line. In this line, Cre is controlled by a fragment of the T (Brachyury) promoter that is active in progenitor cells that reside within the primitive streak and tail bud and which give rise to lineages emerging from these tissues as the embryonic axis extends. However, because TCre-mediated recombination occurs early in development, gene inactivation can result in an axis truncation that precludes the study of gene function in later or more posterior tissues. To address this limitation, we have generated an inducible TCre transgenic mouse line, called TCreERT2, that provides temporal control, through tamoxifen administration, in all cells emerging from the primitive streak or tail bud throughout development. TCreERT2 activity is mostly silent in the absence of tamoxifen and, in its presence, results in near complete recombination of emerging mesoderm from E7.5 through E13.5. We demonstrate the utility of the TCreERT2 line for determining rate of posterior axis extension and somite formation, thus providing the first in vivo tool for such measurements. To test the usefulness of TCreERT2 for genetic manipulation, we demonstrate that an early deletion of ß-Catenin via TCreERT2 induction phenocopies the TCre-mediated deletion of ß-Catenin defect, whereas a later induction bypasses this early phenotype and produces a similar defect in more caudal tissues. TCreERT2 provides a useful and novel tool for the control of gene expression of emerging embryonic lineages throughout development.

  15. Inhibition of Pancreatic Intraepithelial Neoplasia Progression to Carcinoma by Nitric Oxide-Releasing Aspirin in p48Cre/+-LSL-KrasG12D/+ Mice

    Directory of Open Access Journals (Sweden)

    Chinthalapally V. Rao

    2012-09-01

    Full Text Available Nitric oxide-releasing aspirin (NO-aspirin represents a novel class of promising chemopreventive agents. Unlike conventional nonsteroidal anti-inflammatory drugs, NO-aspirin seems to be free of adverse effects while retaining the beneficial activities of its parent compound. The effect of NO-aspirin on pancreatic carcinogenesis was investigated by assessing the development of precursor pancreatic lesions and adenocarcinomas in KrasG12D/+ transgenic mice that recapitulate human pancreatic cancer progression. Six-week-old male p48Cre/+-LSL-KrasG12D/+ transgenic mice (20 per group were fed diets containing 0, 1000, or 2000 ppm NO-aspirin. The development of pancreatic tumors was monitored by positron emission tomography imaging. All mice were killed at the age of 41 weeks and assessed for pancreatic intraepithelial neoplasia (PanIN and pancreatic ductal adenocarcinoma (PDAC and for molecular changes in the tumors. Our results reveal that NO-aspirin at 1000 and 2000 ppm significantly suppressed pancreatic tumor weights, PDAC incidence, and carcinoma in situ (PanIN-3 lesions. The degree of inhibition of PanIN-3 and carcinoma was more pronounced with NO-aspirin at 1000 ppm (58.8% and 48%, respectively than with 2000 ppm (47% and 20%, respectively. NO-aspirin at 1000 ppm significantly inhibited the spread of carcinoma in the pancreas (∼97%; P < .0001. Decreased expression of cyclooxygenase (COX; with ∼42% inhibition of total COX activity, inducible nitric oxide synthase, proliferating cell nuclear antigen, Bcl-2, cyclin D1, and β-catenin was observed, with induction of p21, p38, and p53 in the pancreas of NO-aspirin-treated mice. These results suggest that low-dose NO-aspirin possesses inhibitory activity against pancreatic carcinogenesis by modulating multiple molecular targets.

  16. Store-operated Ca2+ entry regulates Ca2+-activated chloride channels and eccrine sweat gland function.

    Science.gov (United States)

    Concepcion, Axel R; Vaeth, Martin; Wagner, Larry E; Eckstein, Miriam; Hecht, Lee; Yang, Jun; Crottes, David; Seidl, Maximilian; Shin, Hyosup P; Weidinger, Carl; Cameron, Scott; Turvey, Stuart E; Issekutz, Thomas; Meyts, Isabelle; Lacruz, Rodrigo S; Cuk, Mario; Yule, David I; Feske, Stefan

    2016-11-01

    Eccrine sweat glands are essential for sweating and thermoregulation in humans. Loss-of-function mutations in the Ca2+ release-activated Ca2+ (CRAC) channel genes ORAI1 and STIM1 abolish store-operated Ca2+ entry (SOCE), and patients with these CRAC channel mutations suffer from anhidrosis and hyperthermia at high ambient temperatures. Here we have shown that CRAC channel-deficient patients and mice with ectodermal tissue-specific deletion of Orai1 (Orai1K14Cre) or Stim1 and Stim2 (Stim1/2K14Cre) failed to sweat despite normal sweat gland development. SOCE was absent in agonist-stimulated sweat glands from Orai1K14Cre and Stim1/2K14Cre mice and human sweat gland cells lacking ORAI1 or STIM1 expression. In Orai1K14Cre mice, abolishment of SOCE was associated with impaired chloride secretion by primary murine sweat glands. In human sweat gland cells, SOCE mediated by ORAI1 was necessary for agonist-induced chloride secretion and activation of the Ca2+-activated chloride channel (CaCC) anoctamin 1 (ANO1, also known as TMEM16A). By contrast, expression of TMEM16A, the water channel aquaporin 5 (AQP5), and other regulators of sweat gland function was normal in the absence of SOCE. Our findings demonstrate that Ca2+ influx via store-operated CRAC channels is essential for CaCC activation, chloride secretion, and sweat production in humans and mice.

  17. Ciliary neurotrophic factor receptor regulation of adult forebrain neurogenesis.

    Science.gov (United States)

    Lee, Nancy; Batt, Myra K; Cronier, Brigitte A; Jackson, Michele C; Bruno Garza, Jennifer L; Trinh, Dennis S; Mason, Carter O; Spearry, Rachel P; Bhattacharya, Shayon; Robitz, Rachel; Nakafuku, Masato; MacLennan, A John

    2013-01-16

    Appropriately targeted manipulation of endogenous neural stem progenitor (NSP) cells may contribute to therapies for trauma, stroke, and neurodegenerative disease. A prerequisite to such therapies is a better understanding of the mechanisms regulating adult NSP cells in vivo. Indirect data suggest that endogenous ciliary neurotrophic factor (CNTF) receptor signaling may inhibit neuronal differentiation of NSP cells. We challenged subventricular zone (SVZ) cells in vivo with low concentrations of CNTF to anatomically characterize cells containing functional CNTF receptors. We found that type B "stem" cells are highly responsive, whereas type C "transit-amplifying" cells and type A neuroblasts are remarkably unresponsive, as are GFAP(+) astrocytes found outside the SVZ. CNTF was identified in a subset of type B cells that label with acute BrdU administration. Disruption of in vivo CNTF receptor signaling in SVZ NSP cells, with a "floxed" CNTF receptor α (CNTFRα) mouse line and a gene construct driving Cre recombinase (Cre) expression in NSP cells, led to increases in SVZ-associated neuroblasts and new olfactory bulb neurons, as well as a neuron subtype-specific, adult-onset increase in olfactory bulb neuron populations. Adult-onset receptor disruption in SVZ NSP cells with a recombinant adeno-associated virus (AAV-Cre) also led to increased neurogenesis. However, the maintenance of type B cell populations was apparently unaffected by the receptor disruption. Together, the data suggest that endogenous CNTF receptor signaling in type B stem cells inhibits adult neurogenesis, and further suggest that the regulation may occur in a neuron subtype-specific manner.

  18. The Future of Electricity Resource Planning

    Energy Technology Data Exchange (ETDEWEB)

    Kahrl, Fredrich [Energy and Environmental Economics, Inc., San Francisco, CA (United States); Mills, Andrew [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Lavin, Luke [Energy and Environmental Economics, Inc., San Francisco, CA (United States); Ryan, Nancy [Energy and Environmental Economics, Inc., San Francisco, CA (United States); Olsen, Arne [Energy and Environmental Economics, Inc., San Francisco, CA (United States); Schwartz, Lisa [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2016-09-14

    Electricity resource planning is the process of identifying longer-term investments to meet electricity reliability requirements and public policy goals at a reasonable cost. Resource planning processes provide a forum for regulators, electric utilities, and electricity industry stakeholders to evaluate the economic, environmental, and social benefits and costs of different investment options. By facilitating a discussion on future goals, challenges and strategies, resource planning processes often play an important role in shaping utility business decisions. Resource planning emerged more than three decades ago in an era of transition, where declining electricity demand and rising costs spurred fundamental changes in electricity industry regulation and structure. Despite significant changes in the industry, resource planning continues to play an important role in supporting investment decision making. Over the next two decades, the electricity industry will again undergo a period of transition, driven by technological change, shifting customer preferences and public policy goals. This transition will bring about a gradual paradigm shift in resource planning, requiring changes in scope, approaches and methods. Even as it changes, resource planning will continue to be a central feature of the electricity industry. Its functions — ensuring the reliability of high voltage (“bulk”) power systems, enabling oversight of regulated utilities and facilitating low-cost compliance with public policy goals — are likely to grow in importance as the electricity industry enters a new period of technological, economic and regulatory change. This report examines the future of electricity resource planning in the context of a changing electricity industry. The report examines emerging issues and evolving practices in five key areas that will shape the future of resource planning: (1) central-scale generation, (2) distributed generation, (3) demand-side resources, (4

  19. Curbed enthusiasm for electricity reform

    Energy Technology Data Exchange (ETDEWEB)

    Down, E.; Howatson, A.; Hoover, G.; Rheaume, G.

    2003-05-01

    Recent global experiences in electricity restructuring were examined with particular emphasis on how policy decisions have contributed to the current uncertainties for investment in electricity generation and transmission. California, Ontario, Alberta, and the United Kingdom were among the jurisdictions mentioned. It was noted that improved public policies that reduce uncertainties would encourage and manage industry restructuring while achieving improved economics and environmental objectives. Reform of economic regulations has been characterized by the following 3 trends: (1) liberalization, in which prices are determined by competitive markets, (2) state retrenchment, in which activities previously restricted by regulatory barriers are returned to the private sector, and (3) new regulatory design, in which new regulations are introduced to motivate public interest in a more competitive framework. It was noted that electricity is unlike other commodities. It cannot be economically stored and the market is characterized by volatile spot prices. The supply is not inexhaustible either. The electricity market has been more difficult to restructure than any other market that was used as a model for deregulation. No one jurisdiction has been completely successful in electricity restructuring, but each has its own merits and drawbacks. This document also discussed the impact that electricity restructuring will have on the climate change challenge and Canada's commitment under the Kyoto Protocol. When a jurisdiction moves to competitive power generation, generators look for the least expensive generation options, which are not necessarily the most environmentally desirable ones. It was emphasized that there is a need for long-term, integrated planning that addresses policy objectives for market regulation, climate change, and air quality. 38 refs., 1 tab., 1 fig.

  20. DOE Fundamentals Handbook: Electrical Science, Volume 4

    Energy Technology Data Exchange (ETDEWEB)

    1992-06-01

    The Electrical Science Fundamentals Handbook was developed to assist nuclear facility operating contractors provide operators, maintenance personnel, and the technical staff with the necessary fundamentals training to ensure a basic understanding of electrical theory, terminology, and application. The handbook includes information on alternating current (AC) and direct current (DC) theory, circuits, motors, and generators; AC power and reactive transformers; and electrical test components; batteries; AC and DC voltage regulators; instruments and measuring devices. This information will provide personnel with a foundation for understanding the basic operation of various types of DOE nuclear facility electrical equipment.

  1. CTGF mediates Smad-dependent transforming growth factor β signaling to regulate mesenchymal cell proliferation during palate development.

    Science.gov (United States)

    Parada, Carolina; Li, Jingyuan; Iwata, Junichi; Suzuki, Akiko; Chai, Yang

    2013-09-01

    Transforming growth factor β (TGF-β) signaling plays crucial functions in the regulation of craniofacial development, including palatogenesis. Here, we have identified connective tissue growth factor (Ctgf) as a downstream target of the TGF-β signaling pathway in palatogenesis. The pattern of Ctgf expression in wild-type embryos suggests that it may be involved in key processes during palate development. We found that Ctgf expression is downregulated in both Wnt1-Cre; Tgfbr2(fl/fl) and Osr2-Cre; Smad4(fl/fl) palates. In Tgfbr2 mutant embryos, downregulation of Ctgf expression is associated with p38 mitogen-activated protein kinase (MAPK) overactivation, whereas loss of function of Smad4 itself leads to downregulation of Ctgf expression. We also found that CTGF regulates its own expression via TGF-β signaling. Osr2-Cre; Smad4(fl/fl) mice exhibit a defect in cell proliferation similar to that of Tgfbr2 mutant mice, as well as cleft palate. We detected no alteration in bone morphogenetic protein (BMP) downstream targets in Smad4 mutant palates, suggesting that the reduction in cell proliferation is due to defective transduction of TGF-β signaling via decreased Ctgf expression. Significantly, an exogenous source of CTGF was able to rescue the cell proliferation defect in both Tgfbr2 and Smad4 mutant palates. Collectively, our data suggest that CTGF regulates proliferation as a mediator of the canonical pathway of TGF-β signaling during palatogenesis.

  2. Fibroblast growth factor 9 (FGF9)-pituitary homeobox 2 (PITX2) pathway mediates transforming growth factor β (TGFβ) signaling to regulate cell proliferation in palatal mesenchyme during mouse palatogenesis.

    Science.gov (United States)

    Iwata, Jun-ichi; Tung, Lily; Urata, Mark; Hacia, Joseph G; Pelikan, Richard; Suzuki, Akiko; Ramenzoni, Liza; Chaudhry, Obaid; Parada, Carolina; Sanchez-Lara, Pedro A; Chai, Yang

    2012-01-20

    Cleft palate represents one of the most common congenital birth defects. Transforming growth factor β (TGFβ) signaling plays crucial functions in regulating craniofacial development, and loss of TGFβ receptor type II in cranial neural crest cells leads to craniofacial malformations, including cleft palate in mice (Tgfbr2(fl/fl);Wnt1-Cre mice). Here we have identified candidate target genes of TGFβ signaling during palatal formation. These target genes were selected based on combining results from gene expression profiles of embryonic day 14.5 palates from Tgfbr2(fl/fl);Wnt1-Cre mice and previously identified cleft palate phenotypes in genetically engineered mouse models. We found that fibroblast growth factor 9 (Fgf9) and transcription factor pituitary homeobox 2 (Pitx2) expressions are significantly down-regulated in the palate of Tgfbr2(fl/fl);Wnt1-Cre mice, and Fgf9 and Pitx2 loss of function mutations result in cleft palate in mice. Pitx2 expression is down-regulated by siRNA knockdown of Fgf9, suggesting that Fgf9 is upstream of Pitx2. We detected decreased expression of both cyclins D1 and D3 in the palates of Tgfbr2(fl/fl);Wnt1-Cre mice, consistent with the defect in cell proliferation. Significantly, exogenous FGF9 restores expression of cyclins D1 and D3 in a Pitx2-dependent manner and rescues the cell proliferation defect in the palatal mesenchyme of Tgfbr2(fl/fl);Wnt1-Cre mice. Our study indicates that a TGFβ-FGF9-PITX2 signaling cascade regulates cranial neural crest cell proliferation during palate formation.

  3. Electric Vehicle Charging Modeling

    OpenAIRE

    Grahn, Pia

    2014-01-01

    With an electrified passenger transportation fleet, carbon dioxide emissions could be reduced significantly depending on the electric power production mix. Increased electric power consumption due to electric vehicle charging demands of electric vehicle fleets may be met by increased amount of renewable power production in the electrical systems. With electric vehicle fleets in the transportation system there is a need for establishing an electric vehicle charging infrastructure that distribu...

  4. Pten Regulates Epithelial Cytodifferentiation during Prostate Development

    DEFF Research Database (Denmark)

    Lokody, Isabel B; Francis, Jeffrey C; Gardiner, Jennifer R

    2015-01-01

    Gene expression and functional studies have indicated that the molecular programmes involved in prostate development are also active in prostate cancer. PTEN has been implicated in human prostate cancer and is frequently mutated in this disease. Here, using the Nkx3.1:Cre mouse strain and a genetic...... deletion approach, we investigate the role of Pten specifically in the developing mouse prostate epithelia. In contrast to its role in other developing organs, this gene is dispensable for the initial developmental processes such as budding and branching. However, as cytodifferentiation progresses...... that are shared with Pten mutant prostate cancer models, including a decrease in androgen receptor regulated genes. In depth analysis of the phenotype of these mice during development revealed that loss of Pten leads to the precocious differentiation of epithelial cells towards a luminal cell fate. This study...

  5. SUMO: regulating the regulator

    Directory of Open Access Journals (Sweden)

    Bossis Guillaume

    2006-06-01

    Full Text Available Abstract Post-translational modifiers of the SUMO (Small Ubiquitin-related Modifier family have emerged as key regulators of protein function and fate. While the past few years have seen an enormous increase in knowledge on SUMO enzymes, substrates, and consequences of modification, regulation of SUMO conjugation is far from being understood. This brief review will provide an overview on recent advances concerning (i the interplay between sumoylation and other post-translational modifications at the level of individual targets and (ii global regulation of SUMO conjugation and deconjugation.

  6. Electric vehicle utilization for ancillary grid services

    Science.gov (United States)

    Aziz, Muhammad

    2018-02-01

    Electric vehicle has been developed through several decades as transportation mean, without paying sufficient attention of its utilization for other purposes. Recently, the utilization of electric vehicle to support the grid electricity has been proposed and studied intensively. This utilization covers several possible services including electricity storage, spinning reserve, frequency and voltage regulation, and emergency energy supply. This study focuses on theoretical and experimental analysis of utilization of electric vehicles and their used batteries to support a small-scale energy management system. Charging rate of electric vehicle under different ambient temperature (seasonal condition) is initially analyzed to measure the correlation of charging rate, charging time, and state-of-charge. It is confirmed that charging under warmer condition (such as in summer or warmer region) shows higher charging rate than one in colder condition, therefore, shorter charging time can be achieved. In addition, in the demonstration test, each five electric vehicles and used batteries from the same electric vehicles are employed and controlled to support the electricity of the office building. The performance of the system is evaluated throughout a year to measure the load leveling effect during peak-load time. The results show that the targeted peak-load can be shaved well under certain calculated peak-shaving threshold. The finding confirms that the utilization of electric vehicle for supporting the electricity of grid or certain energy management system is feasible and deployable in the future.

  7. Design of Competitive Electricity Markets

    Energy Technology Data Exchange (ETDEWEB)

    2009-07-01

    The design of electricity markets, from pioneers like England and Wales, the Nordic countries, parts of the US, to the more recent additions in Eastern Europe, Africa and South America, carry a lot of similarities. At the same time, there are also distinct differences in the choices made. Is it possible at this stage to conclude that one design works better than the others? In that case - what seems to be working and what remains to be solved? Other important topics to study within this field are optimal investments in interconnection capacities, integration of electricity markets, market power and demand response. The following presentations from the conference are available at the conference web site: Session 1: Market and Politics - from a bird's eye perspective: How liberalised have liberalised electricity markets become?; What is Driving Investments in the Power Sector; Electricity Market Design in the U.S.A.: More Market Or More Politics?; Politics of Energy Policy and Marketing of Energy Markets in the EU - Driving Forces Behind EU Energy Policy and Market Development; Session 2: Experiences from Different Models of Market Design: Using Forward Markets to Improve Electricity Market Design: examples from USA and Colombia; The Texas Deregulated Electricity Market; The Nordic Market Design - A Model For Others?; Designing Wholesale Electricity Markets: Integration with System Operations; Are the British Electricity Trading and Transmission Arrangements Future-Proof?; Session 3: Aspects of Liberalization and its Consequences for - Investments in Interconnection Capacity: Optimal investments in interconnectors and transmission capacity: Lessons from NorNed and the East-West interconnector; Transmission regulation of an integrated energy market; - Integration of Electricity Markets: The Integration of Electricity Markets in Ireland; What would be the ideal support scheme for wind power, in the context of large-scale wind integration and a regional electricity

  8. Competitive intelligence and strategic information management in the regulation of the electric energy distribution market in the state of Mato Grosso do Sul, Brazil; Inteligencia competitiva e gestao de informacao estrategica na regulacao do servico de fornecimento de energia eletrica no estado de Mato Grosso do Sul

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Jenner Luis Puia

    2006-05-15

    This research has as objective the description of information management process in the State Agency of Public Services Regulation from Mato Grosso do Sul (AGEPAN), Brazil, with focus in the strategical decision making as source of competitive intelligence in the regulation of the electric energy distribution market in the state of Mato Grosso do Sul. In the external environment it had been identified the National Agency of Electric Energy, the State Agency of Public Services Regulation from Mato Grosso do Sul, the State, the concessionaires ENERSUL and Elektro and the Society, represented by the Supervision for Consumer's Orientation and Defence (PROCON), Public Prosecution Service, Self-Regulating Body of the Engineering and Architecture Profession, Brazilian |Lawyers Association- MS, as actors in the regulation process. The played roles per item of these actors had been described. The information used by AGEPAN had been defined from the interests and necessities for taking of strategical decision according to Agency's strategical planning. The stages of the management process from these information as source of competitive intelligence in the regulation had been described for the AGEPAN decision makers, from the collection of data by interview in depth with the members of the Decision Advice of the Agency. As referential theoretician for elaboration of the script from the interviews the model of information management proposed by Choo was used. According to the decision makers interviewed, the process of management of the described information did not present evidences that could characterize the decision taking as source of competitive intelligence in the stage of the relative management to the adaptive behavior. (author)

  9. Electric machines

    CERN Document Server

    Gross, Charles A

    2006-01-01

    BASIC ELECTROMAGNETIC CONCEPTSBasic Magnetic ConceptsMagnetically Linear Systems: Magnetic CircuitsVoltage, Current, and Magnetic Field InteractionsMagnetic Properties of MaterialsNonlinear Magnetic Circuit AnalysisPermanent MagnetsSuperconducting MagnetsThe Fundamental Translational EM MachineThe Fundamental Rotational EM MachineMultiwinding EM SystemsLeakage FluxThe Concept of Ratings in EM SystemsSummaryProblemsTRANSFORMERSThe Ideal n-Winding TransformerTransformer Ratings and Per-Unit ScalingThe Nonideal Three-Winding TransformerThe Nonideal Two-Winding TransformerTransformer Efficiency and Voltage RegulationPractical ConsiderationsThe AutotransformerOperation of Transformers in Three-Phase EnvironmentsSequence Circuit Models for Three-Phase Transformer AnalysisHarmonics in TransformersSummaryProblemsBASIC MECHANICAL CONSIDERATIONSSome General PerspectivesEfficiencyLoad Torque-Speed CharacteristicsMass Polar Moment of InertiaGearingOperating ModesTranslational SystemsA Comprehensive Example: The ElevatorP...

  10. The Nordic financial electricity market

    Energy Technology Data Exchange (ETDEWEB)

    2010-11-15

    NordREG is a cooperation of the Nordic energy regulators. The mission is to actively promote legal and institutional framework and conditions necessary for developing the Nordic and European electricity markets. The financial market is an important market for market participants to mitigate their risks. By providing tools for risk management, the financial market contributes to the efficient functioning of both wholesale and end-user markets. NordREG decided during 2009 to undertake a study on the Nordic financial electricity market. The aim of the report is to consider whether any improvements can be made to further increase the efficiency of the Nordic financial electricity market in order to secure an optimal price setting in the wholesale and the end-user markets

  11. Regulation of the power sector

    CERN Document Server

    2013-01-01

    Regulation of the Power Sector is a unified, consistent and comprehensive treatment of the theories and practicalities of regulation in modern power-supply systems. The need for generation to occur at the time of use occasioned by the impracticality of large-scale electricity storage coupled with constant and often unpredictable changes in demand make electricity-supply systems large, dynamic and complex and their regulation a daunting task. Conceptually arranged in four parts, this book addresses both traditional regulatory frameworks and also liberalized and re-regulated environments. First, an introduction gives a full characterization of power supply including engineering, economic and regulatory viewpoints. The second part presents the fundamentals of regulation and the third looks at the regulation of particular components of the power sector in detail. Advanced topics and subjects still open or subject to dispute form the content of the fourth part. In a sector where regulatory design is the key driver...

  12. Electric Vehicles in Imperfect Electricity Markets

    OpenAIRE

    Schill, Wolf-Peter

    2011-01-01

    We use a game-theoretic model to analyze the impacts of a hypothetical fleet of plug-in electric vehicles on the imperfectly competitive German electricity market. Electric vehicles bring both additional demand and additional storage capacity to the market. We determine the effects on prices, welfare, and electricity generation for various cases with different players in charge of vehicle operations. Vehicle loading increases generator profits, but decreases consumer surplus in the power mark...

  13. The Electric Car Challenge.

    Science.gov (United States)

    Diehl, Brian E.

    1997-01-01

    Describes the Electric Car Challenge during which students applied methods of construction to build lightweight, strong vehicles that were powered by electricity. The activity required problem solving, sheet metal work, electricity, design, and construction skills. (JOW)

  14. S/EV 92 (Solar and Electric Vehicles): Proceedings

    Energy Technology Data Exchange (ETDEWEB)

    1992-01-01

    Volume I of these proceedings presents current research on solar and electric powered vehicles. Both fundamental and advanced concepts concerning electric vehicles are presented. The use of photovoltaic cells in electric vehicles and in a broader sense as a means of power generation are discussed. Information on electric powered fleets and races is included. And policy and regulations, especially pertaining to air quality and air pollution abatement are presented.

  15. Agricultural Electricity. Electric Motors. Student Manual.

    Science.gov (United States)

    Benson, Robert T.

    Addressed to the student, this manual, which includes supplementary diagrams, discusses the following topics and principles: Electromagnetic fields, electromagnets, parts of an electric motor, determining speed of an electric motor, types of electric motors in common use (split-phase, capacitor, repulsion-induction, three-phase), the electric…

  16. Electricity in east Asia

    Energy Technology Data Exchange (ETDEWEB)

    Breeze, Paul

    1997-06-01

    Electricity in east Asia highlights all the current regulations and policies and brings you up to data with the latest developments in the power markets in east Asia. In particular, the report assesses utility privatisation and the role and opportunities for independent power producers. It includes: the background to east Asia and its political and economic environment; an overview of the power markets in the region; the natural resources available locally within the region; the power generation technologies being used in the region and the potential for advanced technologies and renewable energy sources; the financial situation, including what opportunities and restraints affect each country; the regulatory structures and political problems associated with private power generation; detailed case studies of the Three Gorges hydropower project in China and the development of nuclear power in east Asia. Electricity in east Asia contains detailed country profiles of China, Hong Kong, Japan, Macao, Mongolia, North Korea, South Korea and Taiwan. It is an indispensable tool for anyone wishing to do business in this highly diverse and exciting region. (Author)

  17. Using fleets of electric-drive vehicles for grid support

    Science.gov (United States)

    Tomić, Jasna; Kempton, Willett

    Electric-drive vehicles can provide power to the electric grid when they are parked (vehicle-to-grid power). We evaluated the economic potential of two utility-owned fleets of battery-electric vehicles to provide power for a specific electricity market, regulation, in four US regional regulation services markets. The two battery-electric fleet cases are: (a) 100 Th!nk City vehicle and (b) 252 Toyota RAV4. Important variables are: (a) the market value of regulation services, (b) the power capacity (kW) of the electrical connections and wiring, and (c) the energy capacity (kWh) of the vehicle's battery. With a few exceptions when the annual market value of regulation was low, we find that vehicle-to-grid power for regulation services is profitable across all four markets analyzed. Assuming now more than current Level 2 charging infrastructure (6.6 kW) the annual net profit for the Th!nk City fleet is from US 7000 to 70,000 providing regulation down only. For the RAV4 fleet the annual net profit ranges from US 24,000 to 260,000 providing regulation down and up. Vehicle-to-grid power could provide a significant revenue stream that would improve the economics of grid-connected electric-drive vehicles and further encourage their adoption. It would also improve the stability of the electrical grid.

  18. Electrical engineer's reference book

    CERN Document Server

    Laughton, M A

    1985-01-01

    Electrical Engineer's Reference Book, Fourteenth Edition focuses on electrical engineering. The book first discusses units, mathematics, and physical quantities, including the international unit system, physical properties, and electricity. The text also looks at network and control systems analysis. The book examines materials used in electrical engineering. Topics include conducting materials, superconductors, silicon, insulating materials, electrical steels, and soft irons and relay steels. The text underscores electrical metrology and instrumentation, steam-generating plants, turbines

  19. DOE handbook electrical safety

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-01-01

    Electrical Safety Handbook presents the Department of Energy (DOE) safety standards for DOE field offices or facilities involved in the use of electrical energy. It has been prepared to provide a uniform set of electrical safety guidance and information for DOE installations to effect a reduction or elimination of risks associated with the use of electrical energy. The objectives of this handbook are to enhance electrical safety awareness and mitigate electrical hazards to employees, the public, and the environment.

  20. Electrical safety guidelines

    Energy Technology Data Exchange (ETDEWEB)

    1993-09-01

    The Electrical Safety Guidelines prescribes the DOE safety standards for DOE field offices or facilities involved in the use of electrical energy. It has been prepared to provide a uniform set of electrical safety standards and guidance for DOE installations in order to affect a reduction or elimination of risks associated with the use of electrical energy. The objectives of these guidelines are to enhance electrical safety awareness and mitigate electrical hazards to employees, the public, and the environment.

  1. TAK1 Regulates Cartilage and Joint Development via the MAPK and BMP Signaling Pathways

    Science.gov (United States)

    Gunnell, Lea M; Jonason, Jennifer H; Loiselle, Alayna E; Kohn, Anat; Schwarz, Edward M; Hilton, Matthew J; O'Keefe, Regis J

    2010-01-01

    The importance of canonical transforming growth factor β (TGF-β) and bone morphogenetic protein (BMP) signaling during cartilage and joint development is well established, but the necessity for noncanonical (SMAD-independent) signaling during these processes is largely unknown. TGF-β activated kinase 1 (TAK1) is a MAP3K activated by TGF-β, BMP, and other mitogen-activated protein kinase (MAPK) signaling components. We set out to define the potential role for noncanonical, TAK1-mediated signaling in cartilage and joint development via deletion of Tak1 in chondrocytes (Col2Cre;Tak1f/f) and the developing limb mesenchyme (Prx1Cre;Tak1f/f). Deletion of Tak1 in chondrocytes resulted in novel embryonic developmental cartilage defects including decreased chondrocyte proliferation, reduced proliferating chondrocyte survival, delayed onset of hypertrophy, reduced Mmp13 expression, and a failure to maintain interzone cells of the elbow joint, which were not observed previously in another Col2Cre;Tak1f/f model. Deletion of Tak1 in limb mesenchyme resulted in widespread joint fusions likely owing to the differentiation of interzone cells to the chondrocyte lineage. The Prx1Cre;Tak1f/f model also allowed us to identify novel columnar chondrocyte organization and terminal maturation defects owing to the interplay between chondrocytes and the surrounding mesenchyme. Furthermore, both our in vivo models and in vitro cell culture studies demonstrate that loss of Tak1 results in impaired activation of the downstream MAPK target p38, as well as diminished activation of the BMP/SMAD signaling pathway. Taken together, these data demonstrate that TAK1 is a critical regulator of both MAPK and BMP signaling and is necessary for proper cartilage and joint development. © 2010 American Society for Bone and Mineral Research. PMID:20213696

  2. Direct and indirect regulation of spinal cord Ia afferent terminal formation by the γ-Protocadherins

    Directory of Open Access Journals (Sweden)

    Tuhina ePrasad

    2011-12-01

    Full Text Available The Pcdh-γ gene cluster encodes 22 protocadherin adhesion molecules that interact as homophilic multimers and critically regulate synaptogenesis and apoptosis of interneurons in the developing spinal cord. Unlike interneurons, the two primary components of the monosynaptic stretch reflex circuit, dorsal root ganglion sensory neurons and ventral motor neurons, do not undergo excessive apoptosis in Pcdh-γdel/del null mutants, which die shortly after birth. However, as we show here, mutants exhibit severely disorganized Ia proprioceptive afferent terminals in the ventral horn. In contrast to the fine net-like pattern observed in wild-type mice, central Ia terminals in Pcdh-γ mutants are expanded, clumped, and fill the space between individual motor neurons; quantitative analysis shows a ~2.5 fold increase in the area of terminals. Concomitant with this, there is a 70% loss of the collaterals that Ia afferents extend to ventral interneurons, many of which undergo apoptosis in the mutants. The Ia afferent phenotype is ameliorated, though not entirely rescued, when apoptosis is blocked in Pcdh-γ null mice by introduction of a Bax null allele. This indicates that loss of ventral interneurons, which act as intermediate Ia afferent targets, contributes to the disorganization of terminals on motor pools. Restricted mutation of the Pcdh-γ cluster using conditional mutants and multiple Cre transgenic lines (Wnt1-Cre for sensory neurons; Pax2-Cre for ventral interneurons; Hb9-Cre for motor neurons also revealed a direct requirement for the γ-Pcdhs in Ia neurons and ventral interneurons, but not in motor neurons themselves. Together, these genetic manipulations indicate that the γ-Pcdhs are required for the formation of the Ia afferent circuit in two ways: First, they control the survival of ventral interneurons that act as intermediate Ia targets; and second, they provide a homophilic molecular cue between Ia afferents and target ventral interneurons.

  3. Law proposal adopted by the Senate, aiming at authorizing the small domestic and non-domestic gas and electricity consumers to have access to or to get back to the regulated tariff; Proposition de loi adoptee par le Senat, tendant a autoriser les petits consommateurs domestiques et non domestiques d'electricite et de gaz naturel a acceder ou a retourner au tarif reglemente

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-03-15

    This unique article aims at modifying some articles of the law no. 2005-781 from July 13 2005 about the setting down of the French energy policy trends. Thanks to this change, the end-users can benefit by the gas and electricity regulated tariff. (J.S.)

  4. Carbon Catabolite Repression Regulates the Production of the Unique Volatile Sodorifen of Serratia plymuthica 4Rx13

    Directory of Open Access Journals (Sweden)

    Nancy Magnus

    2017-12-01

    Full Text Available Microorganisms are capable of synthesizing a plethora of secondary metabolites including the long-overlooked volatile organic compounds. Little knowledge has been accumulated regarding the regulation of the biosynthesis of such mVOCs. The emission of the unique compound sodorifen of Serratia plymuthica isolates was significantly reduced in minimal medium with glucose, while succinate elevated sodorifen release. The hypothesis of carbon catabolite repression (CCR acting as a major control entity on the synthesis of mVOCs was proven by genetic evidence. Central components of the typical CCR of Gram-negative bacteria such as the adenylate cyclase (CYA, the cAMP binding receptor protein (CRP, and the catabolite responsive element (CRE were removed by insertional mutagenesis. CYA, CRP, CRE1 mutants revealed a lower sodorifen release. Moreover, the emission potential of other S. plymuthica isolates was also evaluated.

  5. Electricity market module: Electricity fuel dispatch submodule

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-06-01

    In previous Annual Energy Outlooks (AEO), international electricity trade was represented in the National Energy Modeling System (NEMS) Electricity Market Module (EMM) modeling framework as an exogenous input. The exception to this exogenous treatment was for firm power projections, i.e., new Canadian hydroelectric model builds. The AEO95 implementation of EMM allowed Canadian hydroelectric projects to be selected in the Electricity Capacity Planning (ECP) submodule on an annual basis and otherwise addressed as any other purchased power commitments. This technical memorandum addresses modifications to the Electricity Fuel Dispatch Submodule implemented in AEO96 to enhance the treatment of international electricity trade through the representation of economy imports from Canada.

  6. NV energy electricity storage valuation :

    Energy Technology Data Exchange (ETDEWEB)

    Ellison, James F.; Bhatnagar, Dhruv; Samaan, Nader; Jin, Chunlian

    2013-06-01

    This study examines how grid-level electricity storage may benefit the operations of NV Energy, and assesses whether those benefits are likely to justify the cost of the storage system. To determine the impact of grid-level storage, an hourly production cost model of the Nevada Balancing Authority ("BA") as projected for 2020 was created. Storage was found to add value primarily through the provision of regulating reserve. Certain storage resources were found likely to be cost-effective even without considering their capacity value, as long as their effectiveness in providing regulating reserve was taken into account. Giving fast resources credit for their ability to provide regulating reserve is reasonable, given the adoption of FERC Order 755 ("Pay-for-performance"). Using a traditional five-minute test to determine how much a resource can contribute to regulating reserve does not adequately value fast-ramping resources, as the regulating reserve these resources can provide is constrained by their installed capacity. While an approximation was made to consider the additional value provided by a fast-ramping resource, a more precise valuation requires an alternate regulating reserve methodology. Developing and modeling a new regulating reserve methodology for NV Energy was beyond the scope of this study, as was assessing the incremental value of distributed storage.

  7. Three essays on "making" electric power markets

    Science.gov (United States)

    Kench, Brian Thomas

    2000-10-01

    Technological change over the past three decades has altered most of the basic conditions in the electric power industry. Because of technical progress, the dominant paradigm has shifted from the provision of electric power by regulated and vertically integrated local natural monopolies to competition and vertical separation. In the first essay I provide a historical context of the electric industry's power current deregulation debate. Then a dynamic model of induced institutional change is used to investigate how endogenous technological advancements have induced radical institutional change in the generation and transmission segments of the electric power industry. Because the Federal Energy Regulatory Commission (FERC) ordered regulated utilities to provide open access to their transmission networks and to separate their generation and transmission functions, transmission networks have been used more intensively and in much different ways then in the past. The second essay tests experimentally the predictions of neoclassical theory for a radial electric power market under two alternative deregulated transmission institutions: financial transmission rights and physical transmission rights. Experimental evidence presented there demonstrates that an electric power market with physical transmission rights governing its transmission network generates more "right" market signals relative to a transmission network governed by financial transmission rights. The move to a greater reliance on markets for electric power is an idea that has animated sweeping and dramatic changes in the traditional business of electric power. The third essay examines two of the most innovative and complex initiatives of making electric power markets in the United States: California and PJM. As those markets mature and others are made, they must revise their governance mechanisms to eliminate rules that create inefficiency and adopt rules that work efficiently elsewhere. I argue that

  8. Regulation of Blood Pressure, Appetite, and Glucose by Leptin After Inactivation of Insulin Receptor Substrate 2 Signaling in the Entire Brain or in Proopiomelanocortin Neurons.

    Science.gov (United States)

    do Carmo, Jussara M; da Silva, Alexandre A; Wang, Zhen; Freeman, Nathan J; Alsheik, Ammar J; Adi, Ahmad; Hall, John E

    2016-02-01

    Insulin receptor substrate 2 (IRS2) is one of the 3 major leptin receptor signaling pathways, but its role in mediating the chronic effects of leptin on blood pressure, food intake, and glucose regulation is unclear. We tested whether genetic inactivation of IRS2 in the entire brain (IRS2/Nestin-cre mice) or specifically in proopiomelanocortin (POMC) neurons (IRS2/POMC-cre mice) attenuates the chronic cardiovascular, metabolic, and antidiabetic effects of leptin. Mice were instrumented with telemetry probes for measurement of blood pressure and heart rate and with venous catheters for intravenous infusions. After a 5-day control period, mice received leptin infusion (2 μg/kg per minute) for 7 days. Compared with control IRS2(flox/flox) mice, IRS2/POMC-cre mice had similar body weight and food intake (33±1 versus 35±1 g and 3.6±0.5 versus 3.8±0.2 g per day) but higher mean arterial pressure (MAP) and heart rate (110±2 versus 102±2 mm Hg and 641±9 versus 616±5 bpm). IRS2/Nestin-cre mice were heavier (38±2 g), slightly hyperphagic (4.5±1.0 g per day), and had higher MAP and heart rate (108±2 mm Hg and 659±9 bpm) compared with control mice. Leptin infusion gradually increased MAP despite decreasing food intake by 31% in IRS2(flox/flox) and in Nestin-cre control mice. In contrast, leptin infusion did not change MAP in IRS2/Nestin-cre or IRS2/POMC-cre mice. The anorexic and antidiabetic effects of leptin, however, were similar in all 3 groups. These results indicate that IRS2 signaling in the central nervous system, and particularly in POMC neurons, is essential for the chronic actions of leptin to raise MAP but not for its anorexic or antidiabetic effects. © 2015 American Heart Association, Inc.

  9. X-linked intellectual disability gene CASK regulates postnatal brain growth in a non-cell autonomous manner.

    Science.gov (United States)

    Srivastava, Sarika; McMillan, Ryan; Willis, Jeffery; Clark, Helen; Chavan, Vrushali; Liang, Chen; Zhang, Haiyan; Hulver, Matthew; Mukherjee, Konark

    2016-03-31

    The phenotypic spectrum among girls with heterozygous mutations in the X-linked intellectual disability (XLID) gene CASK (calcium/calmodulin-dependent serine protein kinase) includes postnatal microcephaly, ponto-cerebellar hypoplasia, seizures, optic nerve hypoplasia, growth retardation and hypotonia. Although CASK knockout mice were previously reported to exhibit perinatal lethality and a 3-fold increased apoptotic rate in the brain, CASK deletion was not found to affect neuronal physiology and their electrical properties. The pathogenesis of CASK associated disorders and the potential function of CASK therefore remains unknown. Here, using Cre-LoxP mediated gene excision experiments; we demonstrate that deleting CASK specifically from mouse cerebellar neurons does not alter the cerebellar architecture or function. We demonstrate that the neuron-specific deletion of CASK in mice does not cause perinatal lethality but induces severe recurrent epileptic seizures and growth retardation before the onset of adulthood. Furthermore, we demonstrate that although neuron-specific haploinsufficiency of CASK is inconsequential, the CASK mutation associated human phenotypes are replicated with high fidelity in CASK heterozygous knockout female mice (CASK ((+/-))). These data suggest that CASK-related phenotypes are not purely neuronal in origin. Surprisingly, the observed microcephaly in CASK ((+/-)) animals is not associated with a specific loss of CASK null brain cells indicating that CASK regulates postnatal brain growth in a non-cell autonomous manner. Using biochemical assay, we also demonstrate that CASK can interact with metabolic proteins. CASK knockdown in human cell lines cause reduced cellular respiration and CASK ((+/-)) mice display abnormalities in muscle and brain oxidative metabolism, suggesting a novel function of CASK in metabolism. Our data implies that some phenotypic components of CASK heterozygous deletion mutation associated disorders represent systemic

  10. Report made on behalf of the commission of economic affairs, environment and territory about the proposal of law, adopted in second lecture with modifications by the Senate, relative to the electricity and natural gas regulated tariffs; Rapport fait au nom de la Commission des Affaires Economiques, de l'Environnement et du Territoire sur la proposition de loi, adoptee avec modifications par le Senat en deuxieme lecture, relative aux tarifs reglementes d'electricite et de gaz naturel (n.565)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-01-15

    This text is the conclusion of a long parliamentary procedure with several debates about electricity and natural gas prices, for both individual users and companies. The first article of the proposal of law relative to regulated electricity and gas tariffs, is the only one that remains to be discussed, and concerns the households and the companies with a low power consumption. Examined first at the Senate on October 1, 2007, this proposal of law was debated at the House of Commons on December 11, 2007 and modified for one important point: the possibility for households who have made the choice of a de-regulated energy supplier to change back and benefit again of the regulated tariffs. The adoption of this amendment has led to make some adjustments in the proposal of law which are presented in this document. (J.S.)

  11. Market, Regulation, Market, Regulation

    DEFF Research Database (Denmark)

    Frankel, Christian; Galland, Jean-Pierre

    2015-01-01

    This paper focuses on the European Regulatory system which was settled both for opening the Single Market for products and ensuring the consumers' safety. It claims that the New Approach and Standardization, and the Global Approach to conformity assessment, which suppressed the last technical...... barriers to trade in Europe, realized the free movement of products by organizing progressively several orders of markets and regulation. Based on historical and institutional documents, on technical publications, and on interviews, this article relates how the European Commission and the Member States had...... alternatively recourse to markets and to regulations, at the three main levels of the New Approach Directives implementation. The article focuses also more specifically on the Medical Devices sector, not only because this New Approach sector has long been controversial in Europe, and has recently been concerned...

  12. Electrical installations technology

    CERN Document Server

    Whitfield, J F

    1968-01-01

    Electrical Installations Technology covers the syllabus of the City and Guilds of London Institute course No. 51, the "Electricians B Certificate”. This book is composed of 15 chapters that deal with basic electrical science and electrical installations. The introductory chapters discuss the fundamentals and basic electrical principles, including the concept of mechanics, heat, magnetic fields, electric currents, power, and energy. These chapters also explore the atomic theory of electric current and the electric circuit, conductors, and insulators. The subsequent chapter focuses on the chemis

  13. Swimming Pool Electrical Injuries: Steps Toward Prevention.

    Science.gov (United States)

    Tashiro, Jun; Burnweit, Cathy A

    2017-01-09

    Electrical injuries in swimming pools are an important pediatric public health concern. We sought to (1) improve our understanding of the clinical presentation and outcomes following and (2) describe the epidemiology of swimming pool electrical injuries in the United States. We reviewed 4 cases of pediatric (public (23.9%) and sports facilities (19.1%). Electrical outlets or receptacles (39.8%) were most commonly implicated, followed by electrical system doors (18.2%), electric wiring systems (17.0%), thermostats (16.3%), hair dryers (4.6%), and radios (4.1%). Pediatric cases represented 48.4% of swimming pool-related electrical injuries reported to NEISS. Electrical injuries occurring in and around swimming pools remain an important source of morbidity and mortality. Although NEISS monitors sentinel events, current efforts at preventing such cases are less than adequate. All electrical outlets near swimming pools should be properly wired with ground fault circuit interrupter devices. Possible approaches to increasing safe electrical device installation are through strengthening public awareness and education of the potential for injury, as well as changes to current inspection regulations.

  14. Price volatility in wind dominant electricity markets

    DEFF Research Database (Denmark)

    Farashbashi-Astaneh, Seyed-Mostafa; Chen, Zhe

    2013-01-01

    electricity markets. High price volatility is unappreciated because it imposes high financial risk levels to both electricity consumers and producers. Additionally high price variations impede tracking price signals by consumers in future smart grid and jeopardize implementation of demand response concepts......High penetration of intermittent renewable energy sources causes price volatility in future electricity markets. This is specially the case in European countries that plan high penetration levels. This highlights the necessity for revising market regulations and mechanisms in accordance....... The main contribution of this paper is to quantify volatility patterns of electricity price, as penetration level of wind power increases. Results explain a direct relationship between wind penetration and electricity price volatility in a quantitative manner....

  15. Developing a UAS Program for Electric Utilities

    Science.gov (United States)

    Keltgen, James

    New innovations and technologies using unmanned aerial systems (UAS), or drones, have created unique opportunities for commercial applications. Electric utilities, likewise, realize the benefits of using UAS as a tool in electric utility operations. Although the opportunities exist, establishing a UAS program for electric utilities is largely an endeavor of trial and error or research and development with no clear path defined on how to establish a UAS program. By reviewing UAS use case examples and integrating lessons learned with Federal Aviation Administration (FAA) regulations, UAS best practices, unique electric utility values, legal and insurance perspectives, equipment selection, and thoughtful planning and preparation; a solution model is developed to establish a UAS program for electric utilities.

  16. Nuclear regulation and safety

    Energy Technology Data Exchange (ETDEWEB)

    Hendrie, J.M.

    1982-01-01

    Nuclear regulation and safety are discussed from the standpoint of a hypothetical country that is in the process of introducing a nuclear power industry and setting up a regulatory system. The national policy is assumed to be in favor of nuclear power. The regulators will have responsibility for economic, reliable electric production as well as for safety. Reactor safety is divided into three parts: shut it down, keep it covered, take out the afterheat. Emergency plans also have to be provided. Ways of keeping the core covered with water are discussed. (DLC)

  17. Retinoic acid signalling in thymocytes regulates T cell development

    DEFF Research Database (Denmark)

    Wendland, Kerstin; Sitnik, Katarzyna Maria; Kotarsky, Knut

    The Vitamin A derivative retinoic acid (RA) has emerged as an important regulator of peripheral T cell responses. However, whether there is endogenous retinoic acid receptor (RAR) signaling in developing thymocytes and the potential impact of such signals in thymocyte development remains unclear...... further enhanced in recently generated CD69+ CD4+ SP cells. To address the potential biological significance of RA signaling in developing thymocytes, we evaluated T cell development in CD4Cre-dnRAR mice, where RA signaling is blocked in thymocytes from the CD4+CD8+ double positive (DP) stage onwards due...... of this cell subset. Collectively, our data suggest a direct role for RA signaling in regulating thymocyte homeostasis and T cell development....

  18. Electricity economics. Production functions with electricity

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Zhaoguang [State Grid Energy Research Institute, Beijing (China); Hu, Zheng [Delaware Univ., Newark, DE (United States)

    2013-07-01

    The first book studies on the economics of electricity consumption. Compares the sector production functions with electricity and the commercial production functions with electricity. Introduces the global E-GDP function, the European E-GDP function and 12 national E-GDP functions. Presents the gene characters of EAI production functions and E-GDP functions for USA to see why USA's economy is entering an up-industrialization period. Discusses China's economic growth by production functions with electricity. Electricity Economics: Production Functions with Electricity studies the production output from analyzing patterns of electricity consumption. Since electricity data can be used to measure scenarios of economic performance due to its accuracy and reliability, it could therefore also be used to help scholars explore new research frontiers that directly and indirectly benefits human society. Our research initially explores a similar pattern to substitute the Cobb-Douglas function with the production function with electricity to track and forecast economic activities. The book systematically introduces the theoretical frameworks and mathematical models of economics from the perspective of electricity consumption. The E-GDP functions are presented for case studies of more than 20 developed and developing countries. These functions also demonstrate substantial similarities between human DNA and production functions with electricity in terms of four major characteristics, namely replication, mutation, uniqueness, and evolution. Furthermore, the book includes extensive data and case studies on the U.S., China, Japan, etc. It is intended for scientists, engineers, financial professionals, policy makers, consultants, and anyone else with a desire to study electricity economics as well as related applications.

  19. Influence of serial electrical stimulations of perifornical and posterior hypothalamic orexin-containing neurons on regulation of sleep homeostasis and sleep-wakefulness cycle recovery from experimental comatose state and anesthesia-induced deep sleep.

    Science.gov (United States)

    Chijavadze, E; Chkhartishvili, E; Babilodze, M; Maglakelidze, N; Nachkebia, N

    2013-11-01

    The work was aimed for the ascertainment of following question - whether Orexin-containing neurons of dorsal and lateral hypothalamic, and brain Orexinergic system in general, are those cellular targets which can speed up recovery of disturbed sleep homeostasis and accelerate restoration of sleep-wakefulness cycle phases during some pathological conditions - experimental comatose state and/or deep anesthesia-induced sleep. Study was carried out on white rats. Modeling of experimental comatose state was made by midbrain cytotoxic lesions at intra-collicular level.Animals were under artificial respiration and special care. Different doses of Sodium Ethaminal were used for deep anesthesia. 30 min after comatose state and/or deep anesthesia induced sleep serial electrical stimulations of posterior and/or perifornical hypothalamus were started. Stimulation period lasted for 1 hour with the 5 min intervals between subsequent stimulations applied by turn to the left and right side hypothalamic parts.EEG registration of cortical and hippocampal electrical activity was started immediately after experimental comatose state and deep anesthesia induced sleep and continued continuously during 72 hour. According to obtained new evidences, serial electrical stimulations of posterior and perifornical hypothalamic Orexin-containing neurons significantly accelerate recovery of sleep homeostasis, disturbed because of comatose state and/or deep anesthesia induced sleep. Speed up recovery of sleep homeostasis was manifested in acceleration of coming out from comatose state and deep anesthesia induced sleep and significant early restoration of sleep-wakefulness cycle behavioral states.

  20. Electric circuit theory applied electricity and electronics

    CERN Document Server

    Yorke, R

    1981-01-01

    Electric Circuit Theory provides a concise coverage of the framework of electrical engineering. Comprised of six chapters, this book emphasizes the physical process of electrical engineering rather than abstract mathematics. Chapter 1 deals with files, circuits, and parameters, while Chapter 2 covers the natural and forced response of simple circuit. Chapter 3 talks about the sinusoidal steady state, and Chapter 4 discusses the circuit analysis. The fifth chapter tackles frequency response of networks, and the last chapter covers polyphase systems. This book will be of great help to electrical

  1. VOLTAGE REGULATORS OF SYNCHRONOUS GENERATORS

    OpenAIRE

    Grigorash O. V.; Korzenkov P. G.; Popuchieva M. A.

    2015-01-01

    Synchronous generators are the primary source of electrical power autonomous electrosupply systems, including backup systems. They are also used in a structure of rotating electricity converters and are widely used in renewable energy as part of wind power plants of small, mini and micro hydroelectric plants. Increasing the speed and the accuracy of the system of the voltage regulation of synchronous generators is possible due to the development of combined systems containing more stabilizers...

  2. Electricity market 2001

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-09-01

    The electricity markets in the Nordic countries have undergone major changes since the electricity market reform work was started in the early 1990s. Sweden, Norway and Finland have had a common electricity market since 1996. The work of also reforming the Danish electricity market was begun in the year 2000. The objective of the electricity market reform is to introduce increased competition, to give the consumers greater freedom of choice and also, by open and expanded trade in electricity, create the conditions for efficient pricing. The Swedish National Energy Administration is the supervisory authority as specified in the Electricity Act, and one of the tasks entrusted to it by the Government is to follow developments on the electricity market and to regularly compile and report current market information. The purpose of the 'Electricity market 2001' publication is to meet the need for generalized and readily accessible information on the conditions on the Nordic market. Iceland is not included in the description. The publication also includes summaries of information from recent years concerning electricity generation and utilization in the Nordic countries, the structure of the electricity market from the players' perspective, trade in electricity in the Nordic countries and in Northern Europe, electricity prices in the Nordic and other countries, and the impact of the electricity sector on the environment. The publication contains data on electricity generation and use during the past years, structure of the electricity market, trade in electricity in the Nordic countries and northern Europe, electricity prices in the Nordic countries and other countries as well as impact of electricity generation system on the environment.

  3. PTEN regulates lung endodermal morphogenesis through MEK/ERK pathway.

    Science.gov (United States)

    Xing, Yiming; Wang, Runming; Li, Changgong; Minoo, Parviz

    2015-12-01

    Pten is a multifunctional tumor suppressor. Deletions and mutations in the Pten gene have been associated with multiple forms of human cancers. Pten is a central regulator of several signaling pathways that influences multiple cellular functions. One such function is in cell motility and migration, although the precise mechanism remains unknown. In this study, we deleted Pten in the embryonic lung epithelium using Gata5-cre mice. Absence of Pten blocked branching morphogenesis and ERK and AKT phosphorylation at E12.5. In an explant model, Pten(Δ/Δ) mesenchyme-free embryonic lung endoderm failed to branch. Inhibition of budding in Pten(Δ/Δ) explants was associated with major changes in cell migration, while cell proliferation was not affected. We further examined the role of ERK and AKT in branching morphogenesis by conditional, endodermal-specific mutants which blocked ERK or AKT phosphorylation. MEK(DM/+); Gata5-cre (blocking of ERK phosphorylation) lung showed more severe phenotype in branching morphogenesis. The inhibition of budding was also associated with disruption of cell migration. Thus, the mechanisms by which Pten is required for early endodermal morphogenesis may involve ERK, but not AKT, mediated cell migration. Copyright © 2015 Elsevier Inc. All rights reserved.

  4. Notch Regulates Macrophage-Mediated Inflammation in Diabetic Wound Healing

    Directory of Open Access Journals (Sweden)

    Andrew S. Kimball

    2017-06-01

    Full Text Available Macrophages are essential immune cells necessary for regulated inflammation during wound healing. Recent studies have identified that Notch plays a role in macrophage-mediated inflammation. Thus, we investigated the role of Notch signaling on wound macrophage phenotype and function during normal and diabetic wound healing. We found that Notch receptor and ligand expression are dynamic in wound macrophages during normal healing. Mice with a myeloid-specific Notch signaling defect (DNMAMLfloxedLyz2Cre+ demonstrated delayed early healing (days 1–3 and wound macrophages had decreased inflammatory gene expression. In our physiologic murine model of type 2 diabetes (T2D, Notch receptor expression was significantly increased in wound macrophages on day 6, following the initial inflammatory phase of wound healing, corresponding to increased inflammatory cytokine expression. This increase in Notch1 and Notch2 was also observed in human monocytes from patients with T2D. Further, in prediabetic mice with a genetic Notch signaling defect (DNMAMLfloxedLyz2Cre+ on a high-fat diet, improved wound healing was seen at late time points (days 6–7. These findings suggest that Notch is critical for the early inflammatory phase of wound healing and directs production of macrophage-dependent inflammatory mediators. These results identify that canonical Notch signaling is important in directing macrophage function in wound repair and define a translational target for the treatment of non-healing diabetic wounds.

  5. Regulators of G protein signaling 12 promotes osteoclastogenesis in bone remodeling and pathological bone loss.

    Science.gov (United States)

    Yuan, X; Cao, J; Liu, T; Li, Y-P; Scannapieco, F; He, X; Oursler, M J; Zhang, X; Vacher, J; Li, C; Olson, D; Yang, S

    2015-12-01

    Regulators of G protein signaling (Rgs) have pivotal roles in controlling various cellular processes, such as cell differentiation. How Rgs proteins regulate osteoclast (OC) differentiation, function and bone homeostasis is poorly understood. It was previously demonstrated that Rgs12, the largest protein in the Rgs family, is predominantly expressed in OCs and regulates OC differentiation in vitro. To further understand the role and mechanism of Rgs12 in OC differentiation and bone diseases in vivo, we created OC-targeted Rgs12 knockout mice by using inducible Mx1-Cre and CD11b-Cre. Deletion of Rgs12 in hematopoietic cells or specifically in OC precursors resulted in increased bone mass with decreased OC numbers. Loss of Rgs12 impaired OC differentiation and function with impaired Ca(2+) oscillations and reduced nuclear factor of activated T cells (NFAT) 2 expression. The introduction of wild-type osteoblasts did not rescue the defective osteoclastogenesis. Ectopic expression of NFAT2 rescued defective OC differentiation in CD11b;Rgs12(fl/fl) cells and promoted normal OC differentiation. Moreover, deletion of Rgs12 significantly inhibited pathological osteoclastogenesis and bone destruction in Rgs12-deficient mice that were subjected to ovariectomy and lipodysaccharide for bone loss. Thus our findings demonstrate that Rgs12 is an important regulator in OC differentiation and function and identify Rgs12 as a potential therapeutic target for osteoporosis and inflammation-induced bone loss.

  6. DNMT1 is a Required Genomic Regulator for Murine Liver Histogenesis and Regeneration

    DEFF Research Database (Denmark)

    Kaji, Kosuke; Factor, Valentina M; Andersen, Jesper B

    2016-01-01

    DNA methyltransferase 1 (DNMT1) is an essential regulator maintaining both epigenetic reprogramming during DNA replication and genome stability. We investigated the role of DNMT1 in the regulation of postnatal liver histogenesis under homeostasis and stress conditions. We generated Dnmt1 conditio......DNA methyltransferase 1 (DNMT1) is an essential regulator maintaining both epigenetic reprogramming during DNA replication and genome stability. We investigated the role of DNMT1 in the regulation of postnatal liver histogenesis under homeostasis and stress conditions. We generated Dnmt1...... hepatocytes caused global hypomethylation, enhanced DNA damage response and initiated a senescence state causing a progressive inability to maintain tissue homeostasis and proliferate in response to injury. The liver regenerated via activation and repopulation from progenitors due to lineage......-dependent differences in Alb-Cre expression, providing a basis for selection of less mature and therefore less damaged HPC progeny. Consistently, an efficient knockdown of Dnmt1 in cultured HPCs caused severe DNA damage, cell cycle arrest, senescence and cell death. Mx1-Cre-driven deletion of Dnmt1 in adult quiescent...

  7. Modelling and simulation of the steam line, the high and low pressure turbines and the pressure regulator for the SUN-RAH nucleo electric university simulator; Modelado y simulacion de la linea de vapor, las turbinas de alta y de baja presion y el regulador de presion para el simulador universitario de nucleo electricas SUN RAH

    Energy Technology Data Exchange (ETDEWEB)

    Lopez R, A. [DEPFI, Campus Morelos, en IMTA Jiutepec, Morelos, UNAM (Mexico)]. e-mail: andyskamx@yahoo.com.mx

    2003-07-01

    In the following article the development of a simulator that allows to represent the dynamics of the following systems: steam line, nozzle, vapor separator, reheater, high pressure turbine, low pressure turbine, power generator and the pressure regulator of a nucleo electric power station. We start from the supposition that this plant will be modeled from a nuclear reactor type BWR (Boiling Water Reactor), using models of reduced order that represent the more important dynamic variables of the physical processes that happen along the steam line until the one generator. To be able to carry out the simulation in real time the Mat lab mathematical modeling software is used, as well as the specific simulation tool Simulink. It is necessary to point out that the platform on which the one is executed the simulator is the Windows operating system, to allow the intuitive use that only this operating system offers. The above-mentioned obeys to that the objective of the simulator it is to help the user to understand some of the dynamic phenomena that are present in the systems of a nuclear plant, and to provide a tool of analysis and measurement of variables to predict the desirable behavior of the same ones. The model of a pressure controller for the steam lines, the high pressure turbine and the low pressure turbine is also presented that it will be the one in charge of regulating the demand of the system according to the characteristics and critic restrictions of safety and control, assigned according to those wanted parameters of performance of this system inside the nucleo electric plant. This simulator is totally well defined and it is part of the University student nucleo electric simulator with Boiling Water Reactor (SUN-RAH), an integral project and of greater capacity. (Author)

  8. Power-MOSFET Voltage Regulator

    Science.gov (United States)

    Miller, W. N.; Gray, O. E.

    1982-01-01

    Ninety-six parallel MOSFET devices with two-stage feedback circuit form a high-current dc voltage regulator that also acts as fully-on solid-state switch when fuel-cell out-put falls below regulated voltage. Ripple voltage is less than 20 mV, transient recovery time is less than 50 ms. Parallel MOSFET's act as high-current dc regulator and switch. Regulator can be used wherever large direct currents must be controlled. Can be applied to inverters, industrial furnaces photovoltaic solar generators, dc motors, and electric autos.

  9. Swedish electricity market 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-11-01

    The organization of the Swedish electricity market has been in a state of continual change since the electricity market reform was started in the early 1990s. The conditions for the development of the electricity market have changed since the new Electricity Act came into force on 1 January 1996. The purpose of the reform is to introduce greater competition on the electricity market and provide the consumers with greater freedom of choice and, by open trade in electricity, to create the conditions for more efficient pricing. Being the central energy authority, the Swedish National Board for Industrial and Technical Development, NUTEK, was entrusted by the Government with the task of following developments on the Swedish electricity market. The Network Authority, which has the supervisory function for the new electricity market, were entrusted by the Government with the task of following developments on the Swedish electricity market and regularly compiling and reporting current market information. The new electricity market has now been operative for ten months. The Network Authority has submitted to the Government a detailed report entitled `Developments on the electricity market`, dealing with the experience gained from the electricity market reform. The purpose of the publication is to provide the players on the electricity market - the decision makers, the media and the general public - with comprehensive and easily accessible information on the market conditions. The publication includes summaries of information on electricity production and use in recent years, the structure of the electricity market from the perspective of a player, electricity trade in Sweden and in northern Europe, electricity prices in Sweden and other countries, and the impact of the electricity sector on the environment

  10. Electrical installation calculations advanced

    CERN Document Server

    Kitcher, Christopher

    2013-01-01

    All the essential calculations required for advanced electrical installation workThe Electrical Installation Calculations series has proved an invaluable reference for over forty years, for both apprentices and professional electrical installation engineers alike. The book provides a step-by-step guide to the successful application of electrical installation calculations required in day-to-day electrical engineering practiceA step-by-step guide to everyday calculations used on the job An essential aid to the City & Guilds certificates at Levels 2 and 3For apprentices and electrical installatio

  11. Electrical machines diagnosis

    CERN Document Server

    Trigeassou, Jean-Claude

    2013-01-01

    Monitoring and diagnosis of electrical machine faults is a scientific and economic issue which is motivated by objectives for reliability and serviceability in electrical drives.This book provides a survey of the techniques used to detect the faults occurring in electrical drives: electrical, thermal and mechanical faults of the electrical machine, faults of the static converter and faults of the energy storage unit.Diagnosis of faults occurring in electrical drives is an essential part of a global monitoring system used to improve reliability and serviceability. This diagnosis is perf

  12. Electricity for Road Transport, Flexible Power Systems and Wind Power

    DEFF Research Database (Denmark)

    Nielsen, Lars Henrik; Ravn, Hans; Meibom, Peter

    The aim of the project is to analyse the potential synergistic interplay that may arise between the power sector and the transport sector, if parts of the road transport energy needs are based on electricity via the utilisation of plug-in hybrid electric vehicles and pure electric vehicles......-vehicle connection systems including technical regulation options and analysis of needs for standardisation. 4: Setting up scenarios covering potential developments for utilizing electric drive trains in road transport. Period: Up to year 2030. 5: Analysis of capacity constraints in the electricity grid...

  13. Electric potential and electric field imaging

    Science.gov (United States)

    Generazio, E. R.

    2017-02-01

    The technology and methods for remote quantitative imaging of electrostatic potentials and electrostatic fields in and around objects and in free space is presented. Electric field imaging (EFI) technology may be applied to characterize intrinsic or existing electric potentials and electric fields, or an externally generated electrostatic field made be used for "illuminating" volumes to be inspected with EFI. The baseline sensor technology (e-Sensor) and its construction, optional electric field generation (quasi-static generator), and current e-Sensor enhancements (ephemeral e-Sensor) are discussed. Demonstrations for structural, electronic, human, and memory applications are shown. This new EFI capability is demonstrated to reveal characterization of electric charge distribution creating a new field of study embracing areas of interest including electrostatic discharge (ESD) mitigation, crime scene forensics, design and materials selection for advanced sensors, dielectric morphology of structures, tether integrity, organic molecular memory, and medical diagnostic and treatment efficacy applications such as cardiac polarization wave propagation and electromyography imaging.

  14. The right of energy storage after the energy policy turnaround. The new regulations for electricity storage in EnWG and EEG; Das Recht der Energiespeicherung nach der Energiewende. Die neuen Regelungen zur Stromspeicherung im EnWG und EEG

    Energy Technology Data Exchange (ETDEWEB)

    Sailer, Frank [Stiftung Umweltenergierecht, Wuerzburg (Germany)

    2012-02-15

    The legislation on the energy policy turnaround in the summer of 2011 results not only in legislative changes in the power distribution networks (e.g., paragraph 12a et seq EnWG, NABEG), but also in the electricity storage. Thus the ''law of energy storage'' has been partially revised and for the first time comprehensively codified with a view to the EnWG. The author of the contribution under consideration analyzes this in more detail.

  15. Electricity market 2000

    Energy Technology Data Exchange (ETDEWEB)

    Korsfeldt, T.; Petsala, B.

    2000-08-01

    The electricity markets in the Nordic countries have undergone major changes since the electricity market reform work was started in the early 1990s. Sweden, Norway and Finland have a common electricity market since 1996.The work of also reforming the Danish electricity market was begun in the year 2000. The objective of the electricity market reform is to introduce increased competition,to give the consumers greater freedom of choice and also, by open and expanded trade in electricity, create the conditions for efficient pricing. The Swedish National Energy Administration is the supervisory authority as specified in the Electricity Act, and one of the tasks entrusted to it by the Government is to follow developments on the electricity market and to regularly compile and report current market information. The purpose of the present publication is to meet the need for generalized and readily accessible information on the conditions on the Nordic markets.The publication includes summaries of information from recent years concerning electricity generation and utilization in the Nordic countries, the structure of the electricity market from the players' perspective trade in electricity in the Nordic countries and in Northern Europe, electricity prices in the Nordic and other countries, and the impact of the electricity sector on the environment.

  16. Theft of electricity in Indonesia

    Science.gov (United States)

    Priatna, Dedy Supriadi

    In 1996, among 196 million Indonesian people, only 47.6% had access to electricity; 45.4% in rural areas and 51.5% in urban. 64% of population lived in 61,975 villages, and electric power was supplied to 69% of villages. The government has set a goal to achieve nearly universal services by the year 2014. The government can use existing installed capacity of PLN's system more efficiently. Theft of electricity, which currently constitutes a large share of PLN's losses, should be reduced. The potential of the private sector including captive power and local communities, to participate in electric power generation can also be utilized. The large interest that has been shown by the private sector might be followed by making electric power provision profitable and therefore attractive for the private sector. PLN profits, that in 1996 were only 5.22% instead of the 8% recommended by the World Bank as the best practice for Indonesia, have to be increased by improving their performance levels. The government should also seek solutions for the extremely poor households who will never be able to afford both connection charges and a monthly bill. In 1996 the extremely poor households included 5,251,788 households, constituting 12.1% of the total Indonesian households. Only 1.2% of these households had access to electricity. The objective of this study is to seek the policies that can be implemented in Indonesia that will make it possible to generate and deliver electricity profitably, and reduce theft while providing nearly universal services. For this purpose, the options that are proposed in this study are reducing theft of electricity; something like the CAMPFIRE that has successfully reduced poaching of elephants in Africa: that is, consumer-owned systems, both partially (distribution facilities) and completely (generation and distribution facilities); performance-based regulation (PBR); and solutions for supplying the extremely poor based on the minimum subsidies from the

  17. High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis

    NARCIS (Netherlands)

    Marciniak, Bogumila C.; Pabijaniak, Monika; de Jong, Anne; Dűhring, Robert; Seidel, Gerald; Hillen, Wolfgang; Kuipers, Oscar P.

    2012-01-01

    Background: In Bacillus subtilis and its relatives carbon catabolite control, a mechanism enabling to reach maximal efficiency of carbon and energy sources metabolism, is achieved by the global regulator CcpA (carbon catabolite protein A). CcpA in a complex with HPr-Ser-P (seryl-phosphorylated form

  18. Determining the Effects on Residential Electricity Prices and Carbon Emissions of Electricity Market Restructuring in Alberta

    Science.gov (United States)

    Jahangir, Junaid Bin

    When electricity restructuring initiatives were introduced in Alberta, and finalized with the institution of retail electricity market competition in 2001, it was argued that the changes would deliver lower electricity prices to residential consumers. However, residential electricity prices in Alberta increased dramatically in 2001, and have never returned to their pre-restructuring levels. Proponents of restructuring argue that electricity prices would have been even higher under continued regulation, citing the effect of considerably higher natural gas prices and the roles of other variables. However, many Alberta residential electricity consumers tend to attribute their higher electricity prices to factors such as market power and manipulation associated with restructuring. Since the effects of restructuring on electricity prices cannot be evaluated by simply comparing prices before and after it occurred, the main objective of this thesis is to determine what electricity prices would have been under continued regulation, and to compare them with what was actually observed. To determine these counterfactual electricity prices, a structural model of the determinants of Alberta residential electricity prices is developed, estimated for the prerestructuring period, and used to forecast (counterfactual) prices in the postrestructuring period. However, in forming these forecasts it is necessary to separately account for changes in explanatory variables that could be viewed as occurring due to the restructuring (endogenous) from those changes that would Since the effects of restructuring on electricity prices cannot be evaluated by simply comparing prices before and after it occurred, the main objective of this thesis is to determine what electricity prices would have been under continued regulation, and to compare them with what was actually observed. To determine these counterfactual electricity prices, a structural model of the determinants of Alberta residential

  19. Renewable Electricity Futures (Presentation)

    Energy Technology Data Exchange (ETDEWEB)

    Hand, M. M.

    2012-09-01

    This presentation summarizes findings of NREL's Renewable Electricity Futures study, published in June 2012. RE Futures investigated the challenges and impacts of achieving very high renewable electricity generation levels in the contiguous United States by 2050.

  20. Renewable Electricity Futures (Presentation)

    Energy Technology Data Exchange (ETDEWEB)

    Mai, T.

    2012-10-01

    This presentation library summarizes findings of NREL's Renewable Electricity Futures study, published in June 2012. RE Futures investigated the challenges and impacts of achieving very high renewable electricity generation levels in the contiguous United States by 2050.

  1. Renewable Electricity Futures (Presentation)

    Energy Technology Data Exchange (ETDEWEB)

    Mai, T.

    2012-11-01

    This presentation summarizes findings of NREL's Renewable Electricity Futures study, published in June 2012. RE Futures investigated the challenges and impacts of achieving very high renewable electricity generation levels in the contiguous United States by 2050.

  2. Renewable Electricity Futures (Presentation)

    Energy Technology Data Exchange (ETDEWEB)

    Mai, T.

    2013-04-01

    This presentation summarizes findings of NREL's Renewable Electricity Futures study, published in June 2012. RE Futures investigated the challenges and impacts of achieving very high renewable electricity generation levels in the contiguous United States by 2050.

  3. Home Electrical Safety Checklist

    Science.gov (United States)

    ... bathrooms, and other areas where the risk of electric shock is higher. Consider having GFCIs installed in locations required by the National Electrical Code, such as kitchens, bathrooms, garages, unfinished basements and ...

  4. Electric Power Transmission Lines

    Data.gov (United States)

    Department of Homeland Security — Transmission Lines are the system of structures, wires, insulators and associated hardware that carry electric energy from one point to another in an electric power...

  5. Electricity and Man (Rev.)

    Energy Technology Data Exchange (ETDEWEB)

    Asimov, Isaac

    1974-01-01

    Our whole industrial civilization depends upon cheap energy streaking out in all directions and under fingertip controls—and that would be very difficult without electric current. It would take enormous ingenuity to find substitutes. If all electricity were shut off, then all electronic devices would be dead. Electric lights would darken. Aluminum would become a rare metal. All factories would have to develop power sources on the spot and would have to switch to primitive, mechanical controls. Every house and every farm would have to give up items we have come to take for granted—not only electric lights, but electric irons, electric toasters, electric water pumps, electric control of furnaces, and so on.

  6. Microreactors with electrical fields

    NARCIS (Netherlands)

    Agiral, A.; Gardeniers, Johannes G.E.

    2010-01-01

    The use of electric fields in chemistry is considered an important concept of process intensification. The combination of electricity with chemistry becomes particularly valuable at smaller scales, as they are exploited in microreaction technology. Microreactor systems with integrated electrodes

  7. Annual report 2011 the Norwegian energy regulator

    Energy Technology Data Exchange (ETDEWEB)

    Soeiland, Arne; Lund, Per Tore

    2012-07-01

    The report provides an overview of the current regulation of the electricity and district heating markets in Norway, and explains relevant market developments. An overview of legislative amendments, research work and international participation is also presented in the report (Author)

  8. Electric Field Imaging Project

    Science.gov (United States)

    Wilcutt, Terrence; Hughitt, Brian; Burke, Eric; Generazio, Edward

    2016-01-01

    NDE historically has focused technology development in propagating wave phenomena with little attention to the field of electrostatics and emanating electric fields. This work is intended to bring electrostatic imaging to the forefront of new inspection technologies, and new technologies in general. The specific goals are to specify the electric potential and electric field including the electric field spatial components emanating from, to, and throughout volumes containing objects or in free space.

  9. Electric Vehicle Propulsion System

    OpenAIRE

    Keshri, Ritesh Kumar

    2014-01-01

    Electric vehicles are being considered as one of the pillar of eco-friendly solutions to overcome the problem of global pollution and radiations due to greenhouse gases. Present thesis work reports the improvement in overall performance of the propulsion system of an electric vehicle by improving autonomy and torque-speed characteristic. Electric vehicle propulsion system consists of supply and traction system, and are coordinated by the monitoring & control system. Case of light electric veh...

  10. Electricity restructuring : a comparative review. 2. ed.

    Energy Technology Data Exchange (ETDEWEB)

    Trebilcock, M.J. [Toronto Univ., ON (Canada). Faculty of Law; Hrab, R. [Panel on the Role of Government in Ontario, Toronto, ON (Canada)

    2004-03-01

    Power generation, transmission and distribution are the 3 main components of the electricity market. For many years, most jurisdictions chose to vertically integrate these segments into government or private monopolies. Price controls or rate of return regulations were commonly imposed by governments to prevent the abuse of monopoly power. However, inefficiencies with these arrangements led to poor investment decisions. As a result, many jurisdictions re-evaluated the structure of electricity markets. Restructuring was motivated by the desire to shift investment risk from consumers or taxpayers to producers and investors. The introduction of competitive electricity generation markets created a new sector in the industry. This sector was the retailing or selling of electricity to end-users by retail intermediaries. Although restructuring of the industry should offer more efficient pricing and better-informed consumption and investment decisions, there are many challenges in electricity reform. These challenges include pricing by incumbent generators, transmitters and distributors; discriminatory network access by monopoly transmitters and distributors; inelasticity of electricity supply and demand at peak times; lack of real-time price notification and response by consumers; stranded costs; and, political and consumer resistance to increases in retail prices. The time it takes to get a new power plant online is another issues that complicates new entry in the generation sector. The results of restructuring in Ontario, California, United Kingdom, Pennsylvania, New Jersey, Maryland, Alberta, and Australia were examined. These jurisdictions are trying to find a balance between competition and regulation. 91 refs., 2 figs.

  11. Strategies for Charging Electric Vehicles in the Electricity Market

    DEFF Research Database (Denmark)

    Juul, Nina; Pantuso, Giovanni; Iversen, Jan Emil Banning

    2015-01-01

    This paper analyses different charging strategies for a fleet of electric vehicles. Along with increasing the realism of the strategies, the opportunity for acting on the regulating market is also included. We test the value of a vehicle owner that can choose when and how to charge; by presenting....... We show that all vehicle owners will benefit from acting more intelligently on the energy market. Furthermore, the high value of the stochastic solution shows that, in case the regulating price differs from the expected, the solution to the deterministic problem becomes infeasible....... a model of four alternative charging strategies. We think of them as increasing in sophistication from dumb via delayed to deterministic and stochastic model-based charging. We show that 29% of the total savings from ‘dumb’ are due to delayed charging and that substantial additional gains come charging...

  12. KIR channels tune electrical communication in cerebral arteries

    DEFF Research Database (Denmark)

    Sancho, Maria; Samson, Nina C; Hald, Bjorn O

    2017-01-01

    The conducted vasomotor response reflects electrical communication in the arterial wall and the distance signals spread is regulated by three factors including resident ion channels. This study defined the role of inward-rectifying K(+) channels (KIR) in governing electrical communication along h...... and the increased feedback arising from voltage-dependent-K(+) channels. In summary, this study shows that two KIR populations work collaboratively to govern electrical communication and the spread of vasomotor responses along cerebral arteries.......The conducted vasomotor response reflects electrical communication in the arterial wall and the distance signals spread is regulated by three factors including resident ion channels. This study defined the role of inward-rectifying K(+) channels (KIR) in governing electrical communication along...... hamster cerebral arteries. Focal KCl application induced a vasoconstriction that conducted robustly, indicative of electrical communication among cells. Inhibiting dominant K(+) conductances had no attenuating effect, the exception being Ba(2+) blockade of KIR Electrophysiology and Q-PCR analysis...

  13. Electric Vehicle Technician

    Science.gov (United States)

    Moore, Pam

    2011-01-01

    With President Obama's goal to have one million electric vehicles (EV) on the road by 2015, the electric vehicle technician should have a promising and busy future. "The job force in the car industry is ramping up for a revitalized green car industry," according to Greencareersguide.com. An electric vehicle technician will safely troubleshoot and…

  14. Schneider Electric sozdajot set

    Index Scriptorium Estoniae

    2003-01-01

    Maailma juhtiv elektriseadmete müüja Schneider Electric ühendab oma ettevõtted Balti- ja Põhjamaades uude divisjoni, mille nimeks saab Schneider Nordic Baltic. Eestis luuakse AS Schneider Electric Eesti, mis seni tegutses AS-i Lexel Electric nime all

  15. Electric glass capturing markets

    Energy Technology Data Exchange (ETDEWEB)

    Wikman, K.; Wikstroem, T.

    1996-11-01

    Electric glass has found its place on the construction market. In public buildings, electrically heatable windows are becoming the leading option for large glass walls. Studies on detached houses, both new and renovated, show that floor heating combined with electrically heatable windowpanes is the best choice with respect to resident`s comfort. (orig.)

  16. Future Electricity Markets

    DEFF Research Database (Denmark)

    Pinson, Pierre

    2015-01-01

    The changing face of energy production in Europe necessitates a rethink in the way that electricity markets are structured. The ‘5s’ (Future Electricity Markets) project is a multi-disciplinary project that is looking to challenge the current approach to the design and operation of electricity...... markets as penetration of renewable energy sources increases...

  17. Electric field analysis

    CERN Document Server

    Chakravorti, Sivaji

    2015-01-01

    This book prepares newcomers to dive into the realm of electric field analysis. The book details why one should perform electric field analysis and what are its practical implications. It emphasizes both the fundamentals and modern computational methods of electric machines. The book covers practical applications of the numerical methods in high voltage equipment, including transmission lines, power transformers, cables, and gas insulated systems.

  18. Electric Current Solves Mazes

    Science.gov (United States)

    Ayrinhac, Simon

    2014-01-01

    We present in this work a demonstration of the maze-solving problem with electricity. Electric current flowing in a maze as a printed circuit produces Joule heating and the right way is instantaneously revealed with infrared thermal imaging. The basic properties of electric current can be discussed in this context, with this challenging question:…

  19. Fundamentals of electrical drives

    CERN Document Server

    Veltman, André; De Doncker, Rik W

    2007-01-01

    Provides a comprehensive introduction to various aspects of electrical drive systems. This volume provides a presentation of dynamic generic models that cover all major electrical machine types and modulation/control components of a drive as well as dynamic and steady state analysis of transformers and electrical machines.

  20. REACH. Electricity Units. Secondary.

    Science.gov (United States)

    Smith, Gene; Sappe, Hoyt

    As a part of the REACH (Refrigeration, Electro-Mechanical, Air-Conditioning, Heating) electromechanical cluster, this student manual contains individualized instructional units in the area of electricity. The instructional units focus on electricity fundamentals and electric motors. Each unit follows a typical format that includes a unit sheet,…

  1. Electric and hybrid vehicles

    Science.gov (United States)

    1979-01-01

    Report characterizes state-of-the-art electric and hybrid (combined electric and heat engine) vehicles. Performance data for representative number of these vehicles were obtained from track and dynamometer tests. User experience information was obtained from fleet operators and individual owners of electric vehicles. Data on performance and physical characteristics of large number of vehicles were obtained from manufacturers and available literature.

  2. Electrical Power Converter

    NARCIS (Netherlands)

    Ferreira, J.A.

    2014-01-01

    Electrical power converter for converting electrical power of a power source connected or connectable at an input to electrical DC-power at an output, wherein between the input and the output a first circuit of submodules is provided, wherein said first circuit of submodules and the power source

  3. IEE wiring regulations explained and illustrated

    CERN Document Server

    Scaddan, Brian

    2013-01-01

    The IEE Wiring Regulations Explained and Illustrated, Second Edition discusses the recommendations of the IEE Regulations for the Electrical Equipment of Buildings for the safe selection or erection of wiring installations. The book emphasizes earthing, bonding, protection, and circuit design of electrical wirings. The text reviews the fundamental requirements for safety, earthing systems, the earth fault loop impedance, and supplementary bonding. The book also describes the different types of protection, such as protection against mechanical damage, overcurrent, under voltage (which prevents

  4. International Benchmarking of Electricity Transmission System Operators

    DEFF Research Database (Denmark)

    Agrell, Per J.; Bogetoft, Peter

    2014-01-01

    Electricity transmission system operators (TSO) in Europe are increasing subject to high-powered performance-based regulation, such as revenue-cap regimes. The determination of the parameters in such regimes is challenging for national regulatory authorities (NRA), since there is normally a single...

  5. DC Electric Drive with Autonomous Power Supply

    Directory of Open Access Journals (Sweden)

    V. V. Ramanau

    2006-01-01

    Full Text Available The paper examines a circuit of an impulse DC electric drive with one controlled-rectifier that is a thyristor. Regulation of an average voltage value on an engine is carried out by changing frequency of controlling impulses applied to the thyristor. Calculation of commutating capacitance and inductance is given in the paper.

  6. 29 CFR 1910.302 - Electric utilization systems.

    Science.gov (United States)

    2010-07-01

    ..., roads, etc., or outdoors by established rights on private property. (b) Extent of application—(1... Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR OCCUPATIONAL SAFETY AND HEALTH STANDARDS Electrical Design Safety Standards for...

  7. Tie-2Cre mediated deletion of the vitamin D receptor gene leads to improved skeletal muscle insulin sensitivity and glucose tolerance.

    Science.gov (United States)

    Ni, Wei; Glenn, Denis J; Gardner, David G

    2016-11-01

    A variety of studies have suggested that vitamin D may play a palliative role in improving insulin secretion and glucose tolerance. Endothelial cells of the microcirculation are thought to play an important role in regulating both insulin secretion and insulin sensitivity in target tissues. We have selectively deleted the vitamin D receptor (VDR) gene in endothelial cells of the murine vasculature. These mice demonstrate improved glucose tolerance, improved insulin sensitivity in skeletal muscle, but not in liver, and a reduction in expression and secretion of insulin in the pancreatic islets. Collectively, these data, taken within the context of recent publications in this field, suggest that the endothelial cell VDR plays a tonic inhibitory role in regulating glucose disposal and could prove to be a factor in controlling glucose homeostasis in the intact organism. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Managing electrical safety

    CERN Document Server

    Wiggins, James H, Jr

    2001-01-01

    Managing Electrical Safety provides an overview of electric basics, hazards, and established standards that enables you to understand the hazards you are likely to encounter in your workplace. Focusing on typical industrial environments-which utilize voltages much higher than household or office circuits-the author identifies the eight key components of an electrical safety program and examines each using a model safety management process. You'll learn how to identify electrical hazards, how to prescribe necessary electrical Personal Protective Equipment, how to ensure that equipment is de-ene

  9. Pragmatic electrical engineering fundamentals

    CERN Document Server

    Eccles, William

    2011-01-01

    Pragmatic Electrical Engineering: Fundamentals introduces the fundamentals of the energy-delivery part of electrical systems. It begins with a study of basic electrical circuits and then focuses on electrical power. Three-phase power systems, transformers, induction motors, and magnetics are the major topics.All of the material in the text is illustrated with completely-worked examples to guide the student to a better understanding of the topics. This short lecture book will be of use at any level of engineering, not just electrical. Its goal is to provide the practicing engineer with a practi

  10. Electrical installation calculations basic

    CERN Document Server

    Kitcher, Christopher

    2013-01-01

    All the essential calculations required for basic electrical installation workThe Electrical Installation Calculations series has proved an invaluable reference for over forty years, for both apprentices and professional electrical installation engineers alike. The book provides a step-by-step guide to the successful application of electrical installation calculations required in day-to-day electrical engineering practice. A step-by-step guide to everyday calculations used on the job An essential aid to the City & Guilds certificates at Levels 2 and 3Fo

  11. Development & Implementation of Electric Tram System with Wireless Charging Technology

    OpenAIRE

    DongHo Cho; GuHo Jung; Uooyeol Yoon; Byungsong Lee

    2015-01-01

    In this paper, an electric tram system with a wireless power transfer system based on SMFIR technology is presented. The detailed technology of power-line infra, regulator, and pick-up device is described for train application, respectively. Furthermore, implementation and experimental results for wireless power transfer electric tram are presented

  12. The electricity markets around the Baltic Sea; Elmarknaderna runt Oestersjoen

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-01

    A thorough description of the electric power markets in the countries surrounding the Baltic sea is given in this book. Environmental problems and regulations and nuclear power are surveyed. Factors that may affect an expanded trade of electricity between the countries are analyzed

  13. Development & Implementation of Electric Tram System with Wireless Charging Technology

    Directory of Open Access Journals (Sweden)

    DongHo Cho

    2015-06-01

    Full Text Available In this paper, an electric tram system with a wireless power transfer system based on SMFIR technology is presented. The detailed technology of power-line infra, regulator, and pick-up device is described for train application, respectively. Furthermore, implementation and experimental results for wireless power transfer electric tram are presented

  14. Bmpr1a is required for proper migration of the AVE through regulation of Dkk1 expression in the pre-streak mouse embryo.

    Science.gov (United States)

    Miura, Shigeto; Singh, Ajeet Pratap; Mishina, Yuji

    2010-05-01

    Here, we report a novel mechanism regulating migration of the anterior visceral endoderm (AVE) by BMP signaling through BMPRIA. In Bmpr1a-deficient (Bmpr-null) embryos, the AVE does not migrate at all. In embryos with an epiblast-specific deletion of Bmpr1a (Bmpr1a(null/flox); Sox2Cre embryos), the AVE cells migrate randomly from the distal end of embryos, resulting in an expansion of the AVE. Dkk1, which is normally expressed in the anterior proximal visceral endoderm (PxVE), is downregulated in Bmpr-null embryos, whereas it is circumferentially expressed in Bmpr1a(null/flox); Sox2Cre embryos at E5.75-6.5. These results demonstrate an association of the position of Dkk1 expressing cells with direction of the migration of AVE. In Bmpr1a(null/flox); Sox2Cre embryos, a drastic decrease of WNT signaling is observed at E6.0. Addition of WNT3A to the culture of Bmpr1a(null/flox); Sox2Cre embryos at E5.5 restores expression patterns of Dkk1 and Cer1. These data indicate that BMP signaling in the epiblast induces Wnt3 and Wnt3a expression to maintain WNT signaling in the VE, resulting in downregulation of Dkk1 to establish the anterior expression domain. Thus, our results suggest that BMP signaling regulates the expression patterns of Dkk1 for anterior migration of the AVE. Published by Elsevier Inc.

  15. Klotho expression in long bones regulates FGF23 production during renal failure.

    Science.gov (United States)

    Kaludjerovic, Jovana; Komaba, Hirotaka; Sato, Tadatoshi; Erben, Reinhold G; Baron, Roland; Olauson, Hannes; Larsson, Tobias E; Lanske, Beate

    2017-05-01

    Circulating levels of bone-derived fibroblast growth factor 23 (FGF23) increase early during acute and chronic kidney disease and are associated with adverse outcomes. Membrane-bound Klotho acts as a permissive coreceptor for FGF23, and its expression was recently found in osteoblasts/osteocytes. We hypothesized that Klotho in bone cells is part of an autocrine feedback loop that regulates FGF23 expression during renal failure. Thus, we induced renal failure in mice with targeted deletion of Klotho in long bones. Uremic wild-type (KLfl/fl ) and knockout (Prx1-Cre;KLfl/fl ) mice both responded with reduced body weight, kidney atrophy, hyperphosphatemia, and increased bone turnover. Importantly, long bones of Prx1-Cre;KLfl/fl mice but not their axial skeleton failed to increase FGF23 expression as observed in uremic KLfl/fl mice. Consequently, Prx1-Cre;KLfl/fl mice had significantly lower serum FGF23 and parathyroid hormone levels, and higher renal 1-α-hydroxylase expression, serum 1,25-dihydroxyvitamin D, and calcium levels than KLfl/fl mice. These results were confirmed in two independent models of renal failure, adenine diet induced and 5/6 nephrectomy. Moreover, FGF23-treated bone cells required Klotho to increase FGF23 mRNA and ERK phosphorylation. In summary, our novel findings show that Klotho in bone is crucial for inducing FGF23 production upon renal failure. We propose the presence of an autocrine feedback loop in which Klotho senses the need for FGF23.-Kaludjerovic, J., Komaba, H., Sato, T., Erben, R. G., Baron, R., Olauson, H., Larsson, T. E., Lanske, B. Klotho expression in long bones regulates FGF23 production during renal failure. © FASEB.

  16. Adjustable consumption participating in the electricity markets

    DEFF Research Database (Denmark)

    Biegel, Benjamin; Hansen, Lars Henrik; Stoustrup, Jakob

    2013-01-01

    We consider a player managing a portfolio of flexible demand-side devices and examine the requirements for such a player to become an active player in the Nordic electricity system. In particular, we examine the regulatory requirements that must be satisfied to perform spot price optimization...... and to participate in the regulating power market. To conceptualize these requirements, we estimate the costs per consumer for honoring the given requirements, both under the current regulations but also under the planned future regulations. Finally, we consider a specific case study where domestic appliances...

  17. The Electric Vehicle Development

    DEFF Research Database (Denmark)

    Wang, Jingyu; Liu, Yingqi; Kokko, Ari

    2014-01-01

    In order to respond to the energy crisis and environment problem, countries carry out their research and promotion about electric vehicles. As the ten cities one thousand new energy buses started in 2009, the new energy vehicles have been greatly developed in China, while the development...... of electric vehicles is not that good. This paper selects four cities-Los Angeles, Kanagawa, Hamburg, Amsterdam-that promote electric vehicles successfully and deeply analyzes the development of electric vehicles in these four cities and analyzes the factors that affect the development of electric vehicles...... in three aspects-city environment, government and stakeholders. Then the paper discusses the promotion ways and role of government and consumer. Finally, the paper offers some suggestions to promote electric vehicles in China: focusing on feasibility and adaptability of electric vehicles, playing...

  18. Dynamics of Electricity Demand in Lesotho: A Kalman Filter Approach

    Directory of Open Access Journals (Sweden)

    Thamae Retselisitsoe Isaiah

    2015-04-01

    Full Text Available This study provides an empirical analysis of the time-varying price and income elasticities of electricity demand in Lesotho for the period 1995-2012 using the Kalman filter approach. The results reveal that economic growth has been one of the main drivers of electricity consumption in Lesotho while electricity prices are found to play a less significant role since they are monopoly-driven and relatively low when compared to international standards. These findings imply that increases in electricity prices in Lesotho might not have a significant impact on consumption in the short-run. However, if the real electricity prices become too high over time, consumers might change their behavior and sensitivity to price and hence, energy policymakers will need to reconsider their impact in the long-run. Furthermore, several exogenous shocks seem to have affected the sensitivity of electricity demand during the period prior to regulation, which made individuals, businesses and agencies to be more sensitive to electricity costs. On the other hand, the period after regulation has been characterized by more stable and declining sensitivity of electricity demand. Therefore, factors such as regulation and changes in the country’s economic activities appear to have affected both price and income elasticities of electricity demand in Lesotho.

  19. Digital implementation, simulation and tests in MATLAB of the models of Steam line, the turbines, the pressure regulator of a BWR type nucleo electric power plant; Implementacion digital, simulacion y pruebas en MATLAB de los modelos de la linea de vapor, las turbinas y el regulador de presion de una central Nucleoelectrica tipo BWR

    Energy Technology Data Exchange (ETDEWEB)

    Lopez R, A. [UNAM, Laboratorio de Analisis de Ingenieria de Reactores Nucleares, DEPFI, Campus Morelos, en IMTA Jiutepec, Morelos (Mexico)]. e-mail: andyskamx@yahoo.com.mx

    2004-07-01

    In this phase of the project they were carried out exhaustive tests to the models of the steam lines, turbines and pressure regulator of a BWR type nucleo electric central for to verify that their tendencies and behaviors are it more real possible. For it, it was necessary to also analyze the transfer functions of the different components along the steam line until the power generator. Such models define alone the dominant poles of the system, what is not limitation to reproduce a wide range of anticipated transitoriness of a power station operation. In the same manner, it was integrated and proved the integrated model form with the models of feeding water of the SUN-RAH, simulating the nuclear reactor starting from predetermined entrances of the prospective values of the vessel. Also it was coupled with the graphic interface developed with the libraries DirectX implementing a specific monitoring panel for this system. (Author)

  20. Report made on behalf of the commission of economic affairs about the proposal of law aiming at authorizing the end-users to get back to the regulated power supply tariff, about the proposal of law aiming at authorizing the reversibility of the use of eligibility rights for the purchase of electric power, and about the proposal of law aiming at preserving the purchasing power of households by maintaining the regulated prices of electricity and natural gas; Rapport fait au nom de la commission des Affaires economiques sur la proposition de loi tendant a autoriser les consommateurs particuliers a retourner au tarif reglemente d'electricite, la proposition de loi reversibilite de l'exercice des droits relatifs a l'eligibilite pour l'achat d'energie electrique et la proposition de loi tendant a preserver le pouvoir d'achat des menages en maintenant les tarifs reglementes de vente d'electricite et de gaz naturel

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report concerns the examination of three proposals of law in relation with the regulated prices of electricity and gas. Since July 1, 2007, the French households can freely quit their historical energy supplier and subscribe to other suppliers with de-regulated energy prices. In front of the rise of free energy prices observed during the last years, some households who made the choice of free tariffs have encountered serious financial problems. For this reason, a law is proposed by the commission of economic affairs which aims at allowing the end-users to get back to the regulated tariffs of electricity and gas. (J.S.)

  1. Electric power monthly

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-01

    The Energy Information Administration (EIA) prepares the Electric Power Monthly (EPM) for a wide audience including Congress, Federal and State agencies, the electric utility industry, and the general public. This publication provides monthly statistics for net generation, fossil fuel consumption and stocks, quantity and quality of fossil fuels, cost of fossil fuels, electricity sales, revenue, and average revenue per kilowatthour of electricity sold. Data on net generation, fuel consumption, fuel stocks, quantity and cost of fossil fuels are also displayed for the North American Electric Reliability Council (NERC) regions. The EIA publishes statistics in the EPM on net generation by energy source, consumption, stocks, quantity, quality, and cost of fossil fuels; and capability of new generating units by company and plant. The purpose of this publication is to provide energy decisionmakers with accurate and timely information that may be used in forming various perspectives on electric issues that lie ahead.

  2. Electrical system architecture

    Science.gov (United States)

    Algrain, Marcelo C [Peoria, IL; Johnson, Kris W [Washington, IL; Akasam, Sivaprasad [Peoria, IL; Hoff, Brian D [East Peoria, IL

    2008-07-15

    An electrical system for a vehicle includes a first power source generating a first voltage level, the first power source being in electrical communication with a first bus. A second power source generates a second voltage level greater than the first voltage level, the second power source being in electrical communication with a second bus. A starter generator may be configured to provide power to at least one of the first bus and the second bus, and at least one additional power source may be configured to provide power to at least one of the first bus and the second bus. The electrical system also includes at least one power consumer in electrical communication with the first bus and at least one power consumer in electrical communication with the second bus.

  3. Electric Control Substituting Pitch Control for Large Wind Turbines

    Directory of Open Access Journals (Sweden)

    Jon Kjellin

    2013-01-01

    turbine has fixed pitch and is only controlled electrically accommodated by passive stall of the blades. By electrically controlling the generator rotational speed with the inverter, passive stall regulation is enabled. The first results on experimental verification of stall regulation in gusty wind speeds are presented. The experiments show that the control system can keep the turbine rotational speed constant even at very gusty winds. It is concluded that electrical control accommodated by passive stall is sufficient as control of the wind turbine even at high wind speeds and can substitute mechanical control such as blade pitch.

  4. Electricity reform and sustainable development in China

    Science.gov (United States)

    Williams, James H.; Kahrl, Fredrich

    2008-10-01

    Reducing the environmental impact of supplying electricity is a key to China's sustainable development, and a focus of both domestic and international concerns with greenhouse gas emissions. The environmental performance of the electricity sector is strongly affected by its institutional arrangements: regulatory frameworks, wholesale markets, pricing mechanisms, planning and coordination, and enforcement and incentive mechanisms. These arrangements are set to change as electricity reforms inaugurated in 2002, but sidetracked by several years of supply shortages, are being resumed. In this paper we examine the impact of electricity reform on environmental sustainability by analyzing case studies of four environmental initiatives in the electricity sector: retirement of inefficient generators, installation of pollution control equipment, renewable energy development, and efforts to promote energy efficiency. We find that implementation of these policies falls short of objectives for two main underlying reasons: conflicting priorities between central and provincial governments, and ineffective regulation. Sustainability will be best served not by redoubling short-term supply-oriented, market-based reforms, but by better aligning central and provincial government incentives, and by developing competent, independent regulation at the provincial level. China's central government and sub-national governments in industrialized countries can both contribute to the latter goal.

  5. Electrical and electronic principles

    CERN Document Server

    Knight, S A

    1991-01-01

    Electrical and Electronic Principles, 2, Second Edition covers the syllabus requirements of BTEC Unit U86/329, including the principles of control systems and elements of data transmission. The book first tackles series and parallel circuits, electrical networks, and capacitors and capacitance. Discussions focus on flux density, electric force, permittivity, Kirchhoff's laws, superposition theorem, arrangement of resistors, internal resistance, and powers in a circuit. The text then takes a look at capacitors in circuit, magnetism and magnetization, electromagnetic induction, and alternating v

  6. Essays on electricity theft

    Science.gov (United States)

    Steadman, Keva Ullanda

    Electricity theft is a relatively unknown crime with high economic costs. In this dissertation, I try to identify the factors that motivate persons to steal electricity. First, I use a panel dataset of 97 countries spanning the five year period 2003--2007 to estimate a model that describes the effect of the unemployment rate, GDP per capita, male population and urbanization on theft, robbery and homicide rates. The results are consistent with previous empirical work on crime and economic opportunity. The second and main contribution of this paper is to estimate the same model using distribution loss as a proxy for electricity theft. The results show that the unemployment rate, a key explanatory economic factor for crime, cannot explain variation in electricity theft, although there is some evidence, albeit weak, supporting correlation in rich countries. Therefore, policies that target the reduction of property crime through a reduction in unemployment will have no effect on electricity theft. The third contribution relates to the role that political institutions play in the incentives to steal electricity. After control for socioeconomic factors, I find that there is no relationship between political governance indicators and electricity theft. In the second and third chapters, I compare the United States, a high income, low electricity theft country with Jamaica, a middle income, high electricity theft country. The effect of unemployment on electricity theft mirrors the results found in the first chapter. In the United States, electricity theft is affected by changes in economic conditions but in Jamaica, it is not. These results suggest that the state of institutions plays a role in how social norms are determined. Poor countries will be more likely than rich countries to have weak institutions with social norms that encourage electricity theft.

  7. Principles of electrical safety

    CERN Document Server

    Sutherland, Peter E

    2015-01-01

    Principles of Electrical Safety discusses current issues in electrical safety, which are accompanied by series' of practical applications that can be used by practicing professionals, graduate students, and researchers. .  Provides extensive introductions to important topics in electrical safety Comprehensive overview of inductance, resistance, and capacitance as applied to the human body Serves as a preparatory guide for today's practicing engineers

  8. Semiconductor Electrical Measurements Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — The Semiconductor Electrical Measurements Laboratory is a research laboratory which complements the Optical Measurements Laboratory. The laboratory provides for Hall...

  9. Electrically conductive cellulose composite

    Science.gov (United States)

    Evans, Barbara R.; O'Neill, Hugh M.; Woodward, Jonathan

    2010-05-04

    An electrically conductive cellulose composite includes a cellulose matrix and an electrically conductive carbonaceous material incorporated into the cellulose matrix. The electrical conductivity of the cellulose composite is at least 10 .mu.S/cm at 25.degree. C. The composite can be made by incorporating the electrically conductive carbonaceous material into a culture medium with a cellulose-producing organism, such as Gluconoacetobacter hansenii. The composites can be used to form electrodes, such as for use in membrane electrode assemblies for fuel cells.

  10. Electric power annual 1992

    Energy Technology Data Exchange (ETDEWEB)

    1994-01-06

    The Electric Power Annual presents a summary of electric utility statistics at national, regional and State levels. The objective of the publication is to provide industry decisionmakers, government policymakers, analysts and the general public with historical data that may be used in understanding US electricity markets. The Electric Power Annual is prepared by the Survey Management Division; Office of Coal, Nuclear, Electric and Alternate Fuels; Energy Information Administration (EIA); US Department of Energy. ``The US Electric Power Industry at a Glance`` section presents a profile of the electric power industry ownership and performance, and a review of key statistics for the year. Subsequent sections present data on generating capability, including proposed capability additions; net generation; fossil-fuel statistics; retail sales; revenue; financial statistics; environmental statistics; electric power transactions; demand-side management; and nonutility power producers. In addition, the appendices provide supplemental data on major disturbances and unusual occurrences in US electricity power systems. Each section contains related text and tables and refers the reader to the appropriate publication that contains more detailed data on the subject matter. Monetary values in this publication are expressed in nominal terms.

  11. Electrical Circuit Tester

    Science.gov (United States)

    Love, Frank

    2006-04-18

    An electrical circuit testing device is provided, comprising a case, a digital voltage level testing circuit with a display means, a switch to initiate measurement using the device, a non-shorting switching means for selecting pre-determined electrical wiring configurations to be tested in an outlet, a terminal block, a five-pole electrical plug mounted on the case surface and a set of adapters that can be used for various multiple-pronged electrical outlet configurations for voltages from 100 600 VAC from 50 100 Hz.

  12. Electric vehicle propulsion alternatives

    Science.gov (United States)

    Secunde, R. R.; Schuh, R. M.; Beach, R. F.

    1983-01-01

    Propulsion technology development for electric vehicles is summarized. Analytical studies, technology evaluation, and the development of technology for motors, controllers, transmissions, and complete propulsion systems are included.

  13. Novel Electrically Small Spherical Electric Dipole Antenna

    DEFF Research Database (Denmark)

    Kim, Oleksiy S.

    2010-01-01

    This paper introduces a novel electrically small spherical meander antenna. Horizontal sections of the meander are composed of wire loops, radii of which are chosen so that the whole structure is conformal to a sphere of radius a. To form the meander the loops are connected by wires at a meridian...... plane. The antenna operates as an electric dipole, i.e. it radiates the TM10 spherical mode. The antenna is self-resonant and can be matched to a wide range of input feed lines without an external matching network. In this paper, a spherical meander antenna of the size ka = 0.27 and the input impedance...

  14. Network based management for multiplexed electric vehicle charging

    Science.gov (United States)

    Gadh, Rajit; Chung, Ching Yen; Qui, Li

    2017-04-11

    A system for multiplexing charging of electric vehicles, comprising a server coupled to a plurality of charging control modules over a network. Each of said charging modules being connected to a voltage source such that each charging control module is configured to regulate distribution of voltage from the voltage source to an electric vehicle coupled to the charging control module. Data collection and control software is provided on the server for identifying a plurality of electric vehicles coupled to the plurality of charging control modules and selectively distributing charging of the plurality of charging control modules to multiplex distribution of voltage to the plurality of electric vehicles.

  15. Baseline tests of the Volkswagen transporter electric delivery van

    Science.gov (United States)

    Soltis, R. F.; Mcbrien, E. F.; Bozek, J. M.; Gourash, F.

    1978-01-01

    The Volkswagen Transporter, an electric delivery van, was tested as part of an Energy Research and Development Administration (ERDA) project to characterize the state of the art of electric vehicles. The Volkswagen Transporter is a standard Volkswagen van that has been converted to an electric vehicle. It is powered by a 144-volt traction battery. A direct current (dc) chopper controller, actuated by a conventional accelerator pedal, regulates the voltage or power applied to the 16-kilowatt (21-hp) motor. The braking system uses conventional hydraulic braking in combination with an electric regenerative braking system. The Volkswagen vehicle performance test results are presented.

  16. Brainstem and spinal cord circuitry regulating REM sleep and muscle atonia.

    Directory of Open Access Journals (Sweden)

    Martina Krenzer

    Full Text Available Previous work has suggested, but not demonstrated directly, a critical role for both glutamatergic and GABAergic neurons of the pontine tegmentum in the regulation of rapid eye movement (REM sleep.To determine the in vivo roles of these fast-acting neurotransmitters in putative REM pontine circuits, we injected an adeno-associated viral vector expressing Cre recombinase (AAV-Cre into mice harboring lox-P modified alleles of either the vesicular glutamate transporter 2 (VGLUT2 or vesicular GABA-glycine transporter (VGAT genes. Our results show that glutamatergic neurons of the sublaterodorsal nucleus (SLD and glycinergic/GABAergic interneurons of the spinal ventral horn contribute to REM atonia, whereas a separate population of glutamatergic neurons in the caudal laterodorsal tegmental nucleus (cLDT and SLD are important for REM sleep generation. Our results further suggest that presynaptic GABA release in the cLDT-SLD, ventrolateral periaqueductal gray matter (vlPAG and lateral pontine tegmentum (LPT are not critically involved in REM sleep control.These findings reveal the critical and divergent in vivo role of pontine glutamate and spinal cord GABA/glycine in the regulation of REM sleep and atonia and suggest a possible etiological basis for REM sleep behavior disorder (RBD.

  17. Strategies for Charging Electric Vehicles in the Electricity Market

    Directory of Open Access Journals (Sweden)

    Nina Juul

    2015-06-01

    Full Text Available This paper analyses different charging strategies for a fleet of electric vehicles. Along with increasing the realism of the strategies, the opportunity for acting on the regulating market is also included. We test the value of a vehicle owner that can choose when and how to charge; by presenting a model of four alternative charging strategies. We think of them as increasing in sophistication from dumb via delayed to deterministic and stochastic model-based charging. We show that 29% of the total savings from ‘dumb’ are due to delayed charging and that substantial additional gains come charging optimally in response to predicted spot prices, and – in some settings – additional gains from using the up and down regulating prices. Particularly, strategies are chosen from uncontrolled charging through deterministic optimization, to modelling the charging and bidding problem with stochastic programming. We show that all vehicle owners will benefit from acting more intelligently on the energy market. Furthermore, the high value of the stochastic solution shows that, in case the regulating price differs from the expected, the solution to the deterministic problem becomes infeasible.

  18. Chromatin regulation and gene centrality are essential for controlling fitness pleiotropy in yeast.

    Directory of Open Access Journals (Sweden)

    Linqi Zhou

    2009-11-01

    Full Text Available There are a wide range of phenotypes that are due to loss-of-function or null mutations. Previously, the functions of gene products that distinguish essential from nonessential genes were characterized. However, the functions of products of non-essential genes that contribute to fitness remain minimally understood.Using data from Saccharomyces cerevisiae, we investigated several gene characteristics, which we are able to measure, that are significantly associated with a gene's fitness pleiotropy. Fitness pleiotropy is a measurement of the gene's importance to fitness. These characteristics include: 1 whether the gene's product functions in chromatin regulation, 2 whether the regulation of the gene is influenced by chromatin state, measured by chromatin regulation effect (CRE, 3 whether the gene's product functions as a transcription factor (TF and the number of genes a TF regulates, 4 whether the gene contains TATA-box, and 5 whether the gene's product is central in a protein interaction network. Partial correlation analysis was used to study how these characteristics interact to influence fitness pleiotropy. We show that all five characteristics that were measured are statistically significantly associated with fitness pleiotropy. However, fitness pleiotropy is not associated with the presence of TATA-box when CRE is controlled. In particular, two characteristics: 1 whether the regulation of a gene is more likely to be influenced by chromatin state, and 2 whether the gene product is central in a protein interaction network measured by the number of protein interactions were found to play the most important roles affecting a gene's fitness pleiotropy.These findings highlight the significance of both epigenetic gene regulation and protein interaction networks in influencing the fitness pleiotropy.

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    Science.gov (United States)

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    2010-10-01

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