Applying physical input-output tables of energy to estimate the energy ecological footprint (EEF) of Galicia (NW Spain)

International Nuclear Information System (INIS)

Carballo Penela, Adolfo; Sebastian Villasante, Carlos

2008-01-01

Nowadays, the achievement of sustainable development constitutes an important constraint in the design of energy policies, being necessary the development of reliable indicators to obtain helpful information about the use of energy resources. The ecological footprint (EF) provides a referential framework for the analysis of human demand for bioproductivity, including energy issues. In this article, the theoretical bases of the footprint analysis are described by applying input-output tables of energy to estimate the Galician energy ecological footprint (EEF). It is concluded that the location of highly polluting industries in Galicia makes the Galician EEF quite higher than more developed regions of Spain. The relevance of the outer component of the Galician EEF is also studied. First, available information seems to indicate that the energy incorporated to the trading of manufactured goods would notably increase the Galician consumption of energy. On the other hand, the inclusion of electricity trade in the EEF analysis, including an adjustment, following the same philosophy as with manufactured goods is proposed. This adjustment would substantially reduce the Galician EEF, as the exported electricity widely exceeds the imported one

Skeletal muscle eEF2 and 4EBP1 phosphorylation during endurance exercise is dependent on intensity and muscle fiber type

DEFF Research Database (Denmark)

Rose, Adam John; Bisiani, Bruno; Vistisen, Bodil

2009-01-01

that the increase in skeletal muscle eEF2 Thr(56) phosphorylation was restricted to type I myofibers. Taken together, these data suggest that the depression of skeletal muscle protein synthesis with endurance-type exercise may be regulated at both initiation (i.e. 4EBP1) and elongation (i.e. eEF2) steps, with eEF2......Protein synthesis in skeletal muscle is known to decrease during exercise and it has been suggested that this may depend on the magnitude of the relative metabolic stress within the contracting muscle. To examine the mechanisms behind this, the effect of exercise intensity on skeletal muscle......) increased during exercise but was not influenced by exercise intensity, and was lower than rest 30min after exercise. On the other hand, 4EBP1 phosphorylation at Thr(37/46) decreased during exercise and this decrease was greater at higher exercise intensities, and was similar to rest 30min after exercise...

Loss of translation elongation factor (eEF1A2) expression in vivo differentiates between Wallerian degeneration and dying-back neuronal pathology.

Science.gov (United States)

Murray, Lyndsay M; Thomson, Derek; Conklin, Annalijn; Wishart, Thomas M; Gillingwater, Thomas H

2008-12-01

Wallerian degeneration and dying-back pathology are two well-known cellular pathways capable of regulating the breakdown and loss of axonal and synaptic compartments of neurons in vivo. However, the underlying mechanisms and molecular triggers of these pathways remain elusive. Here, we show that loss of translation elongation factor eEF1A2 expression in lower motor neurons and skeletal muscle fibres in homozygous Wasted mice triggered a dying-back neuropathy. Synaptic loss at the neuromuscular junction occurred in advance of axonal pathology and by a mechanism morphologically distinct from Wallerian degeneration. Dying-back pathology in Wasted mice was accompanied by reduced expression levels of the zinc finger protein ZPR1, as found in other dying-back neuropathies such as spinal muscular atrophy. Surprisingly, experimental nerve lesion revealed that Wallerian degeneration was significantly delayed in homozygous Wasted mice; morphological assessment revealed that approximately 80% of neuromuscular junctions in deep lumbrical muscles at 24 h and approximately 50% at 48 h had retained motor nerve terminals following tibial nerve lesion. This was in contrast to wild-type and heterozygous Wasted mice where < 5% of neuromuscular junctions had retained motor nerve terminals at 24 h post-lesion. These data show that eEF1A2 expression is required to prevent the initiation of dying-back pathology at the neuromuscular junction in vivo. In contrast, loss of eEF1A2 expression significantly inhibited the initiation and progression of Wallerian degeneration in vivo. We conclude that loss of eEF1A2 expression distinguishes mechanisms underlying dying-back pathology from those responsible for Wallerian degeneration in vivo and suggest that eEF1A2-dependent cascades may provide novel molecular targets to manipulate neurodegenerative pathways in lower motor neurons.

Single excitation-emission fluorescence spectrum (EEF) for determination of cetane improver in diesel fuel.

Science.gov (United States)

Insausti, Matías; Fernández Band, Beatriz S

2015-04-05

A highly sensitive spectrofluorimetric method has been developed for the determination of 2-ethylhexyl nitrate in diesel fuel. Usually, this compound is used as an additive in order to improve cetane number. The analytical method consists in building the chemometric model as a first step. Then, it is possible to quantify the analyte with only recording a single excitation-emission fluorescence spectrum (EEF), whose data are introduced in the chemometric model above mentioned. Another important characteristic of this method is that the fuel sample was used without any pre-treatment for EEF. This work provides an interest improvement to fluorescence techniques using the rapid and easily applicable EEF approach to analyze such complex matrices. Exploding EEF was the key to a successful determination, obtaining a detection limit of 0.00434% (v/v) and a limit of quantification of 0.01446% (v/v). Copyright © 2015 Elsevier B.V. All rights reserved.

The utrophin A 5'-UTR drives cap-independent translation exclusively in skeletal muscles of transgenic mice and interacts with eEF1A2.

Science.gov (United States)

Miura, P; Coriati, A; Bélanger, G; De Repentigny, Y; Lee, J; Kothary, R; Holcik, M; Jasmin, B J

2010-04-01

The molecular mechanisms regulating expression of utrophin A are of therapeutic interest since upregulating its expression at the sarcolemma can compensate for the lack of dystrophin in animal models of Duchenne Muscular Dystrophy (DMD). The 5'-UTR of utrophin A has been previously shown to drive cap-independent internal ribosome entry site (IRES)-mediated translation in response to muscle regeneration and glucocorticoid treatment. To determine whether the utrophin A IRES displays tissue specific activity, we generated transgenic mice harboring control (CMV/betaGAL/CAT) or utrophin A 5'-UTR (CMV/betaGAL/UtrA/CAT) bicistronic reporter transgenes. Examination of multiple tissues from two CMV/betaGAL/UtrA/CAT lines revealed that the utrophin A 5'-UTR drives cap-independent translation of the reporter gene exclusively in skeletal muscles and no other examined tissues. This expression pattern suggested that skeletal muscle-specific factors are involved in IRES-mediated translation of utrophin A. We performed RNA-affinity chromatography experiments combined with mass spectrometry to identify trans-factors that bind the utrophin A 5'-UTR and identified eukaryotic elongation factor 1A2 (eEF1A2). UV-crosslinking experiments confirmed the specificity of this interaction. Regions of the utrophin A 5'-UTR that bound eEF1A2 also mediated cap-independent translation in C2C12 muscle cells. Cultured cells lacking eEF1A2 had reduced IRES activity compared with cells overexpressing eEF1A2. Together, these results suggest an important role for eEF1A2 in driving cap-independent translation of utrophin A in skeletal muscle. The trans-factors and signaling pathways driving skeletal-muscle specific IRES-mediated translation of utrophin A could provide unique targets for developing pharmacological-based DMD therapies.

Using docking and alchemical free energy approach to determine the binding mechanism of eEF2K inhibitors and prioritizing the compound synthesis.

Science.gov (United States)

Wang, Qiantao; Edupuganti, Ramakrishna; Tavares, Clint D J; Dalby, Kevin N; Ren, Pengyu

2015-01-01

A-484954 is a known eEF2K inhibitor with submicromolar IC50 potency. However, the binding mechanism and the crystal structure of the kinase remains unknown. Here, we employ a homology eEF2K model, docking and alchemical free energy simulations to probe the binding mechanism of eEF2K, and in turn, guide the optimization of potential lead compounds. The inhibitor was docked into the ATP-binding site of a homology model first. Three different binding poses, hypothesis 1, 2, and 3, were obtained and subsequently applied to molecular dynamics (MD) based alchemical free energy simulations. The calculated relative binding free energy of the analogs of A-484954 using the binding pose of hypothesis 1 show a good correlation with the experimental IC50 values, yielding an r (2) coefficient of 0.96 after removing an outlier (compound 5). Calculations using another two poses show little correlation with experimental data, (r (2) of less than 0.5 with or without removing any outliers). Based on hypothesis 1, the calculated relative free energy suggests that bigger cyclic groups, at R1 e.g., cyclobutyl and cyclopentyl promote more favorable binding than smaller groups, such as cyclopropyl and hydrogen. Moreover, this study also demonstrates the ability of the alchemical free energy approach in combination with docking and homology modeling to prioritize compound synthesis. This can be an effective means of facilitating structure-based drug design when crystal structures are not available.

Neurite outgrowth mediated by translation elongation factor eEF1A1: a target for antiplatelet agent cilostazol.

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Kenji Hashimoto

Full Text Available Cilostazol, a type-3 phosphodiesterase (PDE3 inhibitor, has become widely used as an antiplatelet drug worldwide. A recent second Cilostazol Stroke Prevention Study demonstrated that cilostazol is superior to aspirin for prevention of stroke after an ischemic stroke. However, its precise mechanisms of action remain to be determined. Here, we report that cilostazol, but not the PDE3 inhibitors cilostamide and milrinone, significantly potentiated nerve growth factor (NGF-induced neurite outgrowth in PC12 cells. Furthermore, specific inhibitors for the endoplasmic reticulum protein inositol 1,4,5-triphosphate (IP(3 receptors and several common signaling pathways (PLC-γ, PI3K, Akt, p38 MAPK, and c-Jun N-terminal kinase (JNK, and the Ras/Raf/ERK/MAPK significantly blocked the potentiation of NGF-induced neurite outgrowth by cilostazol. Using a proteomics analysis, we identified that levels of eukaryotic translation elongation factor eEF1A1 protein were significantly increased by treatment with cilostazol, but not cilostamide, in PC12 cells. Moreover, the potentiating effects of cilostazol on NGF-induced neurite outgrowth were significantly antagonized by treatment with eEF1A1 RNAi, but not the negative control of eEF1A1. These findings suggest that eEF1A1 and several common cellular signaling pathways might play a role in the mechanism of cilostazol-induced neurite outgrowth. Therefore, agents that can increase the eEF1A1 protein may have therapeutic relevance in diverse conditions with altered neurite outgrowth.

Regulation of eukaryotic elongation factor 1 alpha (eEF1A) by dynamic lysine methylation

DEFF Research Database (Denmark)

Jakobsson, Magnus E; Małecki, Jędrzej; Falnes, Pål Ø

2018-01-01

Lysine methylation is a frequent post-translational protein modification, which has been intensively studied in the case of histone proteins. Lysine methylations are also found on many non-histone proteins, and one prominent example is eukaryotic elongation factor 1 alpha (eEF1A). Besides its...... essential role in the protein synthesis machinery, a number of non-canonical functions have also been described for eEF1A, such as regulation of the actin cytoskeleton and the promotion of viral replication. The functional significance of the extensive lysine methylations on eEF1A, as well as the identity...

Regulation of DNA methylation on EEF1D and RPL8 expression in cattle.

Science.gov (United States)

Liu, Xuan; Yang, Jie; Zhang, Qin; Jiang, Li

2017-10-01

Dynamic changes to the epigenome play a critical role in a variety of biology processes and complex traits. Many important candidate genes have been identified through our previous genome wide association study (GWAS) on milk production traits in dairy cattle. However, the underlying mechanism of candidate genes have not yet been clearly understood. In this study, we analyzed the methylation variation of the candidate genes, EEF1D and RPL8, which were identified to be strongly associated with milk production traits in dairy cattle in our previous studies, and its effect on protein and mRNA expression. We compared DNA methylation profiles and gene expression levels of EEF1D and RPL8 in five different tissues (heart, liver, mammary gland, ovary and muscle) of three cows. Both genes showed the highest expression level in mammary gland. For RPL8, there was no difference in the DNA methylation pattern in the five tissues, suggesting no effect of DNA methylation on gene expression. For EEF1D, the DNA methylation levels of its first CpG island differed in the five tissues and were negatively correlated with the gene expression levels. To further investigate the function of DNA methylation on the expression of EEF1D, we collected blood samples of three cows at early stage of lactation and in dry period and analyzed its expression and the methylation status of the first CpG island in blood. As a result, the mRNA expression of EEF1D in the dry period was higher than that at the early stage of lactation, while the DNA methylation level in the dry period was lower than that at the early stage of lactation. Our result suggests that the DNA methylation of EEF1D plays an important role in the spatial and temporal regulation of its expression and possibly have an effect on the milk production traits.

Multiple timescale analysis and factor analysis of energy ecological footprint growth in China 1953-2006

International Nuclear Information System (INIS)

Chen Chengzhong; Lin Zhenshan

2008-01-01

Scientific analysis of energy consumption and its influencing factors is of great importance for energy strategy and policy planning. The energy consumption in China 1953-2006 is estimated by applying the energy ecological footprint (EEF) method, and the fluctuation periods of annual China's per capita EEF (EEF cpc ) growth rate are analyzed with the empirical mode decomposition (EMD) method in this paper. EEF intensity is analyzed to depict energy efficiency in China. The main timescales of the 37 factors that affect the annual growth rate of EEF cpc are also discussed based on EMD and factor analysis methods. Results show three obvious undulation cycles of the annual growth rate of EEF cpc , i.e., 4.6, 14.4 and 34.2 years over the last 53 years. The analysis findings from the common synthesized factors of IMF1, IMF2 and IMF3 timescales of the 37 factors suggest that China's energy policy-makers should attach more importance to stabilizing economic growth, optimizing industrial structure, regulating domestic petroleum exploitation and improving transportation efficiency

eEF1A Mediates the Nuclear Export of SNAG-Containing Proteins via the Exportin5-Aminoacyl-tRNA Complex

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José Manuel Mingot

2013-11-01

Full Text Available Exportin5 mediates the nuclear export of double-stranded RNAs, including pre-microRNAs, adenoviral RNAs, and tRNAs. When tRNAs are aminoacylated, the Exportin5-aminoacyl (aa-tRNA complex recruits and coexports the translation elongation factor eEF1A. Here, we show that eEF1A binds to Snail transcription factors when bound to their main target, the E-cadherin promoter, facilitating their export to the cytoplasm in association with the aa-tRNA-Exportin5 complex. Snail binds to eEF1A through the SNAG domain, a protein nuclear export signal present in several transcription factor families, and this binding is regulated by phosphorylation. Thus, we describe a nuclear role for eEF1A and provide a mechanism for protein nuclear export that attenuates the activity of SNAG-containing transcription factors.

Interaction between the cellular protein eEF1A and the 3'-terminal stem-loop of West Nile virus genomic RNA facilitates viral minus-strand RNA synthesis.

Science.gov (United States)

Davis, William G; Blackwell, Jerry L; Shi, Pei-Yong; Brinton, Margo A

2007-09-01

RNase footprinting and nitrocellulose filter binding assays were previously used to map one major and two minor binding sites for the cell protein eEF1A on the 3'(+) stem-loop (SL) RNA of West Nile virus (WNV) (3). Base substitutions in the major eEF1A binding site or adjacent areas of the 3'(+) SL were engineered into a WNV infectious clone. Mutations that decreased, as well as ones that increased, eEF1A binding in in vitro assays had a negative effect on viral growth. None of these mutations affected the efficiency of translation of the viral polyprotein from the genomic RNA, but all of the mutations that decreased in vitro eEF1A binding to the 3' SL RNA also decreased viral minus-strand RNA synthesis in transfected cells. Also, a mutation that increased the efficiency of eEF1A binding to the 3' SL RNA increased minus-strand RNA synthesis in transfected cells, which resulted in decreased synthesis of genomic RNA. These results strongly suggest that the interaction between eEF1A and the WNV 3' SL facilitates viral minus-strand synthesis. eEF1A colocalized with viral replication complexes (RC) in infected cells and antibody to eEF1A coimmunoprecipitated viral RC proteins, suggesting that eEF1A facilitates an interaction between the 3' end of the genome and the RC. eEF1A bound with similar efficiencies to the 3'-terminal SL RNAs of four divergent flaviviruses, including a tick-borne flavivirus, and colocalized with dengue virus RC in infected cells. These results suggest that eEF1A plays a similar role in RNA replication for all flaviviruses.

Eukaryotic elongation factor 2 is a prognostic marker and its kinase a potential therapeutic target in HCC.

Science.gov (United States)

Pott, Leona L; Hagemann, Sascha; Reis, Henning; Lorenz, Kristina; Bracht, Thilo; Herold, Thomas; Skryabin, Boris V; Megger, Dominik A; Kälsch, Julia; Weber, Frank; Sitek, Barbara; Baba, Hideo A

2017-02-14

Hepatocellular carcinoma is a cancer with increasing incidence and largely refractory to current anticancer drugs. Since Sorafenib, a multikinase inhibitor has shown modest efficacy in advanced hepatocellular carcinoma additional treatments are highly needed. Protein phosphorylation via kinases is an important post-translational modification to regulate cell homeostasis including proliferation and apoptosis. Therefore kinases are valuable targets in cancer therapy. To this end we performed 2D differential gel electrophoresis and mass spectrometry analysis of phosphoprotein-enriched lysates of tumor and corresponding non-tumorous liver samples to detect differentially abundant phosphoproteins to screen for novel kinases as potential drug targets. We identified 34 differentially abundant proteins in phosphoprotein enriched lysates. Expression and distribution of the candidate protein eEF2 and its phosphorylated isoform was validated immunohistochemically on 78 hepatocellular carcinoma and non-tumorous tissue samples. Validation showed that total eEF2 and phosphorylated eEF2 at threonine 56 are prognostic markers for overall survival of HCC-patients. The activity of the regulating eEF2 kinase, compared between tumor and non-tumorous tissue lysates by in vitro kinase assays, is more than four times higher in tumor tissues. Functional analyzes regarding eEF2 kinase were performed in JHH5 cells with CRISPR/Cas9 mediated eEF2 kinase knock out. Proliferation and growth is decreased in eEF2 kinase knock out cells. eEF2 and phosphorylated eEF2 are prognostic markers for survival of hepatocellular carcinoma patients and the regulating eEF2 kinase is a potential drug target for tumor therapy.

RPL13A and EEF1A1 Are Suitable Reference Genes for qPCR during Adipocyte Differentiation of Vascular Stromal Cells from Patients with Different BMI and HOMA-IR.

Science.gov (United States)

Gentile, Adriana-Mariel; Lhamyani, Said; Coín-Aragüez, Leticia; Oliva-Olivera, Wilfredo; Zayed, Hatem; Vega-Rioja, Antonio; Monteseirin, Javier; Romero-Zerbo, Silvana-Yanina; Tinahones, Francisco-José; Bermúdez-Silva, Francisco-Javier; El Bekay, Rajaa

2016-01-01

Real-time or quantitative PCR (qPCR) is a useful technique that requires reliable reference genes for data normalization in gene expression analysis. Adipogenesis is among the biological processes suitable for this technique. The selection of adequate reference genes is essential for qPCR gene expression analysis of human Vascular Stromal Cells (hVSCs) during their differentiation into adipocytes. To the best of our knowledge, there are no studies validating reference genes for the analyses of visceral and subcutaneous adipose tissue hVSCs from subjects with different Body Mass Index (BMI) and Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index. The present study was undertaken to analyze this question. We first analyzed the stability of expression of five potential reference genes: CYC, GAPDH, RPL13A, EEF1A1, and 18S ribosomal RNA, during in vitro adipogenic differentiation, in samples from these types of patients. The expression of RPL13A and EEF1A1 was not affected by differentiation, thus being these genes the most stable candidates, while CYC, GAPDH, and 18S were not suitable for this sort of analysis. This work highlights that RPL13A and EEF1A1 are good candidates as reference genes for qPCR analysis of hVSCs differentiation into adipocytes from subjects with different BMI and HOMA-IR.

eEF1A Controls ascospore differentiation through elevated accuracy, but controls longevity and fruiting body formation through another mechanism in Podospora anserina.

Science.gov (United States)

Silar, P; Lalucque, H; Haedens, V; Zickler, D; Picard, M

2001-01-01

Antisuppressor mutations in the eEF1A gene of Podospora anserina were previously shown to impair ascospore formation, to drastically increase life span, and to permit the development of the Crippled Growth degenerative process. Here, we show that eEF1A controls ascospore formation through accuracy level maintenance. Examination of antisuppressor mutant perithecia reveals two main cytological defects, mislocalization of spindle and nuclei and nuclear death. Antisuppression levels are shown to be highly dependent upon both the mutation site and the suppressor used, precluding any correlation between antisuppression efficiency and severity of the sporulation impairment. Nevertheless, severity of ascospore differentiation defect is correlated with resistance to paromomycin. We also show that eEF1A controls fruiting body formation and longevity through a mechanism(s) different from accuracy control. In vivo, GFP tagging of the protein in a way that partly retains its function confirmed earlier cytological observation; i.e., this factor is mainly diffuse within the cytosol, but may transiently accumulate within nuclei or in defined regions of the cytoplasm. These data emphasize the fact that the translation apparatus exerts a global regulatory control over cell physiology and that eEF1A is one of the key factors involved in this monitoring. PMID:11514440

Structural rationale for the cross-resistance of tumor cells bearing the A399V variant of elongation factor eEF1A1 to the structurally unrelated didemnin B, ternatin, nannocystin A and ansatrienin B

Science.gov (United States)

Sánchez-Murcia, Pedro A.; Cortés-Cabrera, Álvaro; Gago, Federico

2017-10-01

At least four classes of structurally distinct natural products with potent antiproliferative activities target the translation elongation factor eEF1A1, which is best known as the G-protein that delivers amino acyl transfer RNAs (aa-tRNAs) to ribosomes during mRNA translation. We present molecular models in atomic detail that provide a common structural basis for the high-affinity binding of didemnin B, ternatin, ansatrienin B and nannocystin A to eEF1A1, as well as a rationale based on molecular dynamics results that accounts for the deleterious effect of replacing alanine 399 with valine. The proposed binding site, at the interface between domains I and III, is eminently hydrophobic and exists only in the GTP-bound conformation. Drug binding at this site is expected to disrupt neither loading of aa-tRNAs nor GTP hydrolysis but would give rise to stabilization of this particular conformational state, in consonance with reported experimental findings. The experimental solution of the three-dimensional structure of mammalian eEF1A1 has proved elusive so far and the highly homologous eEF1A2 from rabbit muscle has been crystallized and solved only as a homodimer in a GDP-bound conformation. Interestingly, in this dimeric structure the large interdomain cavity where the drugs studied are proposed to bind is occupied by a mostly hydrophobic α-helix from domain I of the same monomer. Since binding of this α-helix and any of these drugs to domain III of eEF1A(1/2) is, therefore, mutually exclusive and involves two distinct protein conformations, one intriguing possibility that emerges from our study is that the potent antiproliferative effect of these natural products may arise not only from inhibition of protein synthesis, which is the current dogma, but also from interference with some other non-canonical functions. From this standpoint, this type of drugs could be considered antagonists of eEF1A1/2 oligomerization, a hypothesis that opens up novel areas of research.

Fluoxetine induces autophagic cell death via eEF2K-AMPK-mTOR-ULK complex axis in triple negative breast cancer.

Science.gov (United States)

Sun, Dejuan; Zhu, Lingjuan; Zhao, Yuqian; Jiang, Yingnan; Chen, Lixia; Yu, Yang; Ouyang, Liang

2018-04-01

Triple negative breast cancer (TNBC) is a complex and intrinsically aggressive tumour with poor prognosis, and the discovery of targeted small-molecule drugs for TNBC treatment still remains in its infancy. In this study, we aimed to discover a small-molecule agent for TNBC treatment and illuminate its potential mechanisms. Cell viability was detected by using methylthiazoltetrazolium (MTT) assay. Electron microscopy, GFP-LC3 transfection, monodansylcadaverine staining and apoptosis assay were performed to determine Fluoxetine-induced autophagy and apoptosis. Western blotting and siRNA transfection were carried out to investigate the mechanisms of Fluoxetine-induced autophagy. iTRAQ-based proteomics analysis was used to explore the underlying mechanisms. We have demonstrated that Fluoxetine had remarkable anti-proliferative activities and induced autophagic cell death in MDA-MB-231 and MDA-MB-436 cells. The mechanism for Fluoxetine-induced autophagic cell death was associated with inhibition of eEF2K and activation of AMPK-mTOR-ULK complex axis. Further iTRAQ-based proteomics and network analyses revealed that Fluoxetine-induced mechanism was involved in BIRC6, BNIP1, SNAP29 and Bif-1. These results demonstrate that Fluoxetine induces apoptosis and autophagic cell death in TNBC, which will hold a promise for the future TNBC therapy. © 2017 John Wiley & Sons Ltd.

Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8

International Nuclear Information System (INIS)

De Bortoli, Massimiliano; Man, Tsz-Kwong; Rao, Pulivarthi H; Kim, John YH; Castellino, Robert C; Lu, Xin-Yan; Deyo, Jeffrey; Sturla, Lisa Marie; Adesina, Adekunle M; Perlaky, Laszlo; Pomeroy, Scott L; Lau, Ching C

2006-01-01

Medulloblastoma is the most common malignant brain tumor of childhood. Improvements in clinical outcome require a better understanding of the genetic alterations to identify clinically significant biological factors and to stratify patients accordingly. In the present study, we applied cytogenetic characterization to guide the identification of biologically significant genes from gene expression microarray profiles of medulloblastoma. We analyzed 71 primary medulloblastomas for chromosomal copy number aberrations (CNAs) using comparative genomic hybridization (CGH). Among 64 tumors that we previously analyzed by gene expression microarrays, 27 were included in our CGH series. We analyzed clinical outcome with respect to CNAs and microarray results. We filtered microarray data using specific CNAs to detect differentially expressed candidate genes associated with survival. The most frequent lesions detected in our series involved chromosome 17; loss of 16q, 10q, or 8p; and gain of 7q or 2p. Recurrent amplifications at 2p23-p24, 2q14, 7q34, and 12p13 were also observed. Gain of 8q is associated with worse overall survival (p = 0.0141), which is not entirely attributable to MYC amplification or overexpression. By applying CGH results to gene expression analysis of medulloblastoma, we identified three 8q-mapped genes that are associated with overall survival in the larger group of 64 patients (p < 0.05): eukaryotic translation elongation factor 1D (EEF1D), ribosomal protein L30 (RPL30), and ribosomal protein S20 (RPS20). The complementary use of CGH and expression profiles can facilitate the identification of clinically significant candidate genes involved in medulloblastoma growth. We demonstrate that gain of 8q and expression levels of three 8q-mapped candidate genes (EEF1D, RPL30, RPS20) are associated with adverse outcome in medulloblastoma

Actividades del grupo EEF en el contexto de la red europea TMR "Polarimetría radar: Teoría y aplicaciones"

OpenAIRE

Fabregas Canovas, Francisco Javier; López Martínez, Carlos; Broquetas Ibars, Antoni

2000-01-01

The activity of the EEF group in the TMR European Project Radar Polarimetry: Theory and Applications will be presented in this paper. We have developed new polarimetric-interferometric retrieval algorithms and enhancement techniques. These methods will be showed briefly in the next points. Peer Reviewed

Prenatal low-dose methylmercury exposure impairs neurite outgrowth and synaptic protein expression and suppresses TrkA pathway activity and eEF1A1 expression in the rat cerebellum

Energy Technology Data Exchange (ETDEWEB)

Fujimura, Masatake, E-mail: fujimura@nimd.go.jp [Department of Basic Medical Sciences, National Institute for Minamata Disease, Kumamoto (Japan); Usuki, Fusako [Department of Clinical Medicine, National Institute for Minamata Disease, Kumamoto (Japan); Cheng, Jinping; Zhao, Wenchang [School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240 (China)

2016-05-01

Methylmercury (MeHg) is a highly neurotoxic environmental chemical that can cause developmental impairments. Human fetuses and neonates are particularly susceptible to MeHg toxicity; however, the mechanisms governing its effects in the developing brain are unclear. In the present study, we investigated the effects of prenatal and lactational MeHg exposure on the developing cerebellum in rats. We demonstrated that exposure to 5 ppm MeHg decreased postnatal expression of pre- and postsynaptic proteins, suggesting an impairment in synaptic development. MeHg exposure also reduced neurite outgrowth, as shown by a decrease in the expression of the neurite marker neurofilament H. These changes were not observed in rats exposed to 1 ppm MeHg. In order to define the underlying mechanism, we investigated the effects of MeHg exposure on the tropomyosin receptor kinase (Trk) A pathway, which plays important roles in neuronal differentiation and synapse formation. We demonstrated suppression of the TrkA pathway on gestation day 20 in rats exposed to 5 ppm MeHg. In addition, down-regulation of eukaryotic elongation factor 1A1 (eEF1A1) was observed on postnatal day 1. eEF1A1 knockdown in differentiating PC12 cells impaired neurite outgrowth and synaptic protein expression, similar to the results of MeHg exposure in the cerebellum. These results suggest that suppression of the TrkA pathway and subsequent decreases in eEF1A1 expression induced by prenatal exposure to MeHg may lead to reduced neurite outgrowth and synaptic protein expression in the developing cerebellum. - Highlights: • Prenatal exposure to MeHg decreased postnatal expression of synaptic proteins. • MeHg exposure also reduced neurite outgrowth postnatally. • Suppression of the TrkA pathway and eEF1A1 expression was induced by MeHg exposure. • eEF1A1 knockdown impaired neurite outgrowth and synaptic protein expression.

Prenatal low-dose methylmercury exposure impairs neurite outgrowth and synaptic protein expression and suppresses TrkA pathway activity and eEF1A1 expression in the rat cerebellum

International Nuclear Information System (INIS)

Fujimura, Masatake; Usuki, Fusako; Cheng, Jinping; Zhao, Wenchang

2016-01-01

Methylmercury (MeHg) is a highly neurotoxic environmental chemical that can cause developmental impairments. Human fetuses and neonates are particularly susceptible to MeHg toxicity; however, the mechanisms governing its effects in the developing brain are unclear. In the present study, we investigated the effects of prenatal and lactational MeHg exposure on the developing cerebellum in rats. We demonstrated that exposure to 5 ppm MeHg decreased postnatal expression of pre- and postsynaptic proteins, suggesting an impairment in synaptic development. MeHg exposure also reduced neurite outgrowth, as shown by a decrease in the expression of the neurite marker neurofilament H. These changes were not observed in rats exposed to 1 ppm MeHg. In order to define the underlying mechanism, we investigated the effects of MeHg exposure on the tropomyosin receptor kinase (Trk) A pathway, which plays important roles in neuronal differentiation and synapse formation. We demonstrated suppression of the TrkA pathway on gestation day 20 in rats exposed to 5 ppm MeHg. In addition, down-regulation of eukaryotic elongation factor 1A1 (eEF1A1) was observed on postnatal day 1. eEF1A1 knockdown in differentiating PC12 cells impaired neurite outgrowth and synaptic protein expression, similar to the results of MeHg exposure in the cerebellum. These results suggest that suppression of the TrkA pathway and subsequent decreases in eEF1A1 expression induced by prenatal exposure to MeHg may lead to reduced neurite outgrowth and synaptic protein expression in the developing cerebellum. - Highlights: • Prenatal exposure to MeHg decreased postnatal expression of synaptic proteins. • MeHg exposure also reduced neurite outgrowth postnatally. • Suppression of the TrkA pathway and eEF1A1 expression was induced by MeHg exposure. • eEF1A1 knockdown impaired neurite outgrowth and synaptic protein expression.

Targeted silencing of elongation factor 2 kinase suppresses growth and sensitizes tumors to doxorubicin in an orthotopic model of breast cancer.

Directory of Open Access Journals (Sweden)

Ibrahim Tekedereli

Full Text Available Eukaryotic elongation factor 2 kinase (eEF-2K, through its phosphorylation of elongation factor 2 (eEF2, provides a mechanism by which cells can control the rate of the elongation phase of protein synthesis. The activity of eEF-2K is increased in rapidly proliferating malignant cells, is inhibited during mitosis, and may contribute to the promotion of autophagy in response to anti-cancer therapies. The purpose of this study was to examine the therapeutic potential of targeting eEF-2K in breast cancer tumors. Through the systemic administration of liposomal eEF-2K siRNA (twice a week, i.v. 150 µg/kg, the expression of eEF-2K was down-regulated in vivo in an orthotopic xenograft mouse model of a highly aggressive triple negative MDA-MB-231 tumor. This targeting resulted in a substantial decrease in eEF2 phosphorylation in the tumors, and led to the inhibition of tumor growth, the induction of apoptosis and the sensitization of tumors to the chemotherapy agent doxorubicin. eEF-2K down-modulation in vitro resulted in a decrease in the expression of c-Myc and cyclin D1 with a concomitant increase in the expression of p27(Kip1. A decrease in the basal activity of c-Src (phospho-Tyr-416, focal adhesion kinase (phospho-Tyr-397, and Akt (phospho-Ser-473 was also detected following eEF-2K down-regulation in MDA-MB-231 cells, as determined by Western blotting. Where tested, similar results were seen in ER-positive MCF-7 cells. These effects were also accompanied by a decrease in the observed invasive phenotype of the MDA-MB-231 cells. These data support the notion that the disruption of eEF-2K expression in breast cancer cells results in the down-regulation of signaling pathways affecting growth, survival and resistance and has potential as a therapeutic approach for the treatment of breast cancer.

Mitochondrial Respiration Inhibitors Suppress Protein Translation and Hypoxic Signaling via the Hyperphosphorylation and Inactivation of Translation Initiation Factor eIF2α and Elongation Factor eEF2

Science.gov (United States)

Li, Jun; Mahdi, Fakhri; Du, Lin; Datta, Sandipan; Nagle, Dale G.; Zhou, Yu-Dong

2011-01-01

Over 20000 lipid extracts of plants and marine organisms were evaluated in a human breast tumor T47D cell-based reporter assay for hypoxia-inducible factor-1 (HIF-1) inhibitory activity. Bioassay-guided isolation and dereplication-based structure elucidation of an active extract from the Bael tree (Aegle marmelos) afforded two protolimonoids, skimmiarepin A (1) and skimmiarepin C (2). In T47D cells, 1 and 2 inhibited hypoxia-induced HIF-1 activation with IC50 values of 0.063 µM and 0.068 µM, respectively. Compounds 1 and 2 also suppressed hypoxic induction of the HIF-1 target genes GLUT-1 and VEGF. Mechanistic studies revealed that 1 and 2 inhibited HIF-1 activation by blocking the hypoxia-induced accumulation of HIF-1α protein. At the range of concentrations that inhibited HIF-1 activation, 1 and 2 suppressed cellular respiration by selectively inhibiting the mitochondrial electron transport chain at complex I (NADH dehydrogenase). Further investigation indicated that mitochondrial respiration inhibitors such as 1 and rotenone induced the rapid hyperphosphorylation and inhibition of translation initiation factor eIF2α and elongation factor eEF2. The inhibition of protein translation may account for the short-term exposure effects exerted by mitochondrial inhibitors on cellular signaling, while the suppression of cellular ATP production may contribute to the inhibitory effects following extended treatment periods. PMID:21875114

Isolation of Flavonoids from Deguelia duckeana and Their Effect on Cellular Viability, AMPK, eEF2, eIF2 and eIF4E

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Lorena M. C. Cursino

2016-02-01

Full Text Available Preparations of Deguelia duckeana, known in Brazil as timbó, are used by indigenous people to kill fish. Reinvestigation of its extracts resulted in the isolation and identification of 11 known flavonoids identified as 3,5,4’-trimethoxy-4-prenylstilbene (1, 4-methoxyderricidine (2, lonchocarpine (3, 4-hydroxylonchocarpine (4, 4-methoxylonchocarpine (5, 5-hydroxy-4’,7-dimethoxy-6-prenylflavanone (6, 4’-hydroxyisolonchocarpine (7, 4’-methoxyisolonchocarpine (8, 3’,4’,7-trimethoxyflavone (9, 3’,4’-methylenedioxy-7-methoxyflavone (10, and 2,2-dimethyl-chromone-5,4’-hydroxy-5’-methoxyflavone (11. Except for 1, 3, and 4 all of these flavonoids have been described for the first time in D. duckeana and the flavanone 6 for the first time in nature. Compounds 2, 3, 4, 7, 9, and 10 were studied for their potential to induce cell death in neuronal SK-N-SH cells. Only the chalcone 4 and the flavanone 7 significantly induced lactate dehydrogenase (LDH release, which was accompanied by activation of caspase-3 and impairment of energy homeostasis in the MTT assay and may explain the killing effect on fish. Interestingly, the flavone 10 reduced cell metabolism in the MTT assay without inducing cytotoxicity in the LDH assay. Furthermore, the flavonoids 2, 3, 4, 7, and 10 induced phosphorylation of the AMP-activated protein kinase (AMPK and the eukaryotic elongation factor 2 (eEF2. The initiation factor eIF4E was dephosphorylated in the presence of these compounds. The initiation factor eIF2alpha was not affected. Further studies are needed to elucidate the importance of the observed effects on protein synthesis and potential therapeutic perspectives.

Elongation factor 1 alpha1 and genes associated with Usher syndromes are downstream targets of GBX2.

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David A Roeseler

Full Text Available Gbx2 encodes a DNA-binding transcription factor that plays pivotal roles during embryogenesis. Gain-and loss-of-function studies in several vertebrate species have demonstrated a requirement for Gbx2 in development of the anterior hindbrain, spinal cord, inner ear, heart, and neural crest cells. However, the target genes through which GBX2 exerts its effects remain obscure. Using chromatin immunoprecipitation coupled with direct sequencing (ChIP-Seq analysis in a human prostate cancer cell line, we identified cis-regulatory elements bound by GBX2 to provide insight into its direct downstream targets. The analysis revealed more than 286 highly significant candidate target genes, falling into various functional groups, of which 51% are expressed in the nervous system. Several of the top candidate genes include EEF1A1, ROBO1, PLXNA4, SLIT3, NRP1, and NOTCH2, as well as genes associated with the Usher syndrome, PCDH15 and USH2A, and are plausible candidates contributing to the developmental defects in Gbx2(-/- mice. We show through gel shift analyses that sequences within the promoter or introns of EEF1A1, ROBO1, PCDH15, USH2A and NOTCH2, are directly bound by GBX2. Consistent with these in vitro results, analyses of Gbx2(-/- embryos indicate that Gbx2 function is required for migration of Robo1-expressing neural crest cells out of the hindbrain. Furthermore, we show that GBX2 activates transcriptional activity through the promoter of EEF1A1, suggesting that GBX2 could also regulate gene expression indirectly via EEF1A. Taken together, our studies show that GBX2 plays a dynamic role in development and diseases.

Cloning, sequencing, and transgenic expression of Podospora curvicolla and Sordaria macrospora eEF1A genes: relationship between cytosolic translation and longevity in filamentous fungi.

Science.gov (United States)

Gagny, B; Rossignol, M; Silar, P

1997-12-01

We have cloned and sequenced the gene encoding the translation elongation factor eEF1A from two filamentous fungi, Podospora curvicolla and Sordaria macrospora. These fungi are close relatives of Podospora anserina and also show senescence syndromes. Comparison of the sequences of the deduced proteins with that of P. anserina reveals that the three proteins differ in several positions. Replacement of the P. anserina gene by either of the two exogenous genes does not entail any modification in P. anserina physiology; the longevity of the fungus is not affected. No alteration of in vivo translational accuracy was detected; however, the exogenous proteins nonetheless promoted a modification of the resistance to the aminoglycoside antibiotic paromomycin. These data suggest that optimization of life span between these closely related fungi has likely not been performed during evolution through modifications of eEF1A activity, despite the fact that mutations in this factor can drastically affect longevity. Copyright 1997 Academic Press.

A high-throughput screening assay for eukaryotic elongation factor 2 kinase inhibitors

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Ting Xiao

2016-10-01

Full Text Available Eukaryotic elongation factor 2 kinase (eEF2K inhibitors may aid in the development of new therapeutic agents to combat cancer. Purified human eEF2K was obtained from an Escherichia coli expression system and a luminescence-based high-throughput screening (HTS assay was developed using MH-1 peptide as the substrate. The luminescent readouts correlated with the amount of adenosine triphosphate remaining in the kinase reaction. This method was applied to a large-scale screening campaign against a diverse compound library and subsequent confirmation studies. Nine initial hits showing inhibitory activities on eEF2K were identified from 56,000 synthetic compounds during the HTS campaign, of which, five were chosen to test their effects in cancer cell lines.

The life and death of translation elongation factor 2

DEFF Research Database (Denmark)

Jørgensen, Rene; Merrill, A.R.; Andersen, Gregers Rom

2006-01-01

The eukaryotic elongation factor 2 (eEF2) occupies an essential role in protein synthesis where it catalyses the translocation of the two tRNAs and the mRNA after peptidyl transfer on the 80S ribosome. Recent crystal structures of eEF2 and the cryo-EM reconstruction of its 80S complex now provide...... diphthamide residue, which is ADP-ribosylated by diphtheria toxin from Corynebacterium diphtheriae and exotoxin A from Pseudomonas aeruginosa....

Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp

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Fei Mo

2014-09-01

Full Text Available Reference genes are commonly used for normalization of target gene expression during RT-qPCR analysis. However, no housekeeping genes or reference genes have been identified to be stable across different tissue types or under different experimental conditions. To identify the most suitable reference genes for RT-qPCR analysis of target gene expression in the hepatopancreas of crucian carp (Carassius auratus under various conditions (sex, age, water temperature, and drug treatments, seven reference genes, including beta actin (ACTB, beta-2 microglobulin (B2M, embryonic elongation factor-1 alpha (EEF1A, glyceraldehyde phosphate dehydrogenase (GAPDH, alpha tubulin (TUBA, ribosomal protein l8 (RPL8 and glucose-6-phosphate dehydrogenase (G6PDH, were evaluated in this study. The stability and ranking of gene expression were analyzed using three different statistical programs: GeNorm, Normfinder and Bestkeeper. The expression errors associated with selection of the genes were assessed by the relative quantity of CYP4T. The results indicated that all the seven genes exhibited variability under the experimental conditions of this research, and the combination of ACTB/TUBA/EEF1A or of ACTB/EEF1A was the best candidate that raised the accuracy of quantitative analysis of gene expression. The findings highlighted the importance of validation of housekeeping genes for research on gene expression under different conditions of experiment and species.

RESEARCH INVESTIGATIONS ON THE PROTEOME: 1. MECHANISMS OF REGULATING PROTEIN SYNTHESIS, AND 2. GLOBAL CHARACTERIZATION OF PROTEOMIC RESPONSES TO ARSENIC EXPOSURES

Science.gov (United States)

Eukaryotic Elongation Factor 2 (eEF2) mediates translocation in protein synthesis. eEF2 is modified by two post-translational modifications: the phosphorylation of Thr57 in the G domain and a unique conversion of His699 to diphthamide at the tip of domain IV. Diphthamide is the t...

The Level of Autoantibodies Targeting Eukaryote Translation Elongation Factor 1 α1 and Ubiquitin-Conjugating Enzyme 2L3 in Nondiabetic Young Adults

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Eunhee G. Kim

2016-01-01

Full Text Available BackgroundThe prevalence of novel type 1 diabetes mellitus (T1DM antibodies targeting eukaryote translation elongation factor 1 alpha 1 autoantibody (EEF1A1-AAb and ubiquitin-conjugating enzyme 2L3 autoantibody (UBE2L3-AAb has been shown to be negatively correlated with age in T1DM subjects. Therefore, we aimed to investigate whether age affects the levels of these two antibodies in nondiabetic subjects.MethodsEEF1A1-AAb and UBE2L3-AAb levels in nondiabetic control subjects (n=150 and T1DM subjects (n=101 in various ranges of age (18 to 69 years were measured using an enzyme-linked immunosorbent assay. The cutoff point for the presence of each autoantibody was determined based on control subjects using the formula: [mean absorbance+3×standard deviation].ResultsIn nondiabetic subjects, there were no significant correlations between age and EEF1A1-AAb and UBE2L3-AAb levels. However, there was wide variation in EEF1A1-AAb and UBE2L3-AAb levels among control subjects <40 years old; the prevalence of both EEF1A1-AAb and UBE2L3-AAb in these subjects was 4.4%. When using cutoff points determined from the control subjects <40 years old, the prevalence of both autoantibodies in T1DM subjects was decreased (EEFA1-AAb, 15.8% to 8.9%; UBE2L3-AAb, 10.9% to 7.9% when compared to the prevalence using the cutoff derived from the totals for control subjects.ConclusionThere was no association between age and EEF1A1-AAb or UBE2L3-AAb levels in nondiabetic subjects. However, the wide variation in EEF1A1-AAb and UBE2L3-AAb levels apparent among the control subjects <40 years old should be taken into consideration when determining the cutoff reference range for the diagnosis of T1DM.

Safety analysis - current and future regulatory challenges

Energy Technology Data Exchange (ETDEWEB)

Jamieson, T., E-mail: Terry.Jamieson@cnsc-ccsn.gc.ca [Canadian Nuclear Safety Commission, Ottawa, ON (Canada)

2015-07-01

'Full text:' The current and future regulatory challenges associated with deterministic safety analysis are reviewed, including: 1. The CNSC's and safety control areas. 2. Traditional safety analysis approach. 3. Experience gained and impact. 4. Current analysis and regulatory approaches. 5. Current status. 6. Complexity and challenges In particular, the technical, regulatory and strategic aspects of these challenges are discussed. (author)

Safety analysis - current and future regulatory challenges

International Nuclear Information System (INIS)

Jamieson, T.

2015-01-01

'Full text:' The current and future regulatory challenges associated with deterministic safety analysis are reviewed, including: 1. The CNSC's and safety control areas. 2. Traditional safety analysis approach. 3. Experience gained and impact. 4. Current analysis and regulatory approaches. 5. Current status. 6. Complexity and challenges In particular, the technical, regulatory and strategic aspects of these challenges are discussed. (author)

Antioxidant Activity of Flaxseed (Linum usitatissimum L. shell and Analysis of Its Polyphenol Contents by LC-MS/MS

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Hatica Han

2018-07-01

Full Text Available Flaxseed (Linum usitatissimum L. is important source of oil and protein for industrial, pharmaceutical, and nutritional applications. In order to estimate the effects of lyophilized aqueous extract of flaxseed shell (AEF and evaporated ethanolic extract of flaxseed shell (EEF, we studied their DPPH, ABTS, DMPD and O 2 •- scavenging effects. Total antioxidant activity by ferric thiocyanate method, Fe 3+, Cu 2+ and [Fe 3+-(TPTZ 2] 3+ reducing ability, and Fe 2+ chelating activity. Also, α-tocopherol, BHA, trolox, and BHT were used as positive controls. The results clearly AEF and EEF demonstrated effective antioxidant activity. The quantity of p-hydroxybenzoic, vanillin, p-coumaric acid, ascorbic acid, ferulic acid, and ellagic acid were investigated by LC-MS/MS. The present study will introduce a novelty for further studies on the antioxidant effects of AEF and EEF.

A Ca2+-calmodulin-eEF2K-eEF2 signalling cascade, but not AMPK, contributes to the suppression of skeletal muscle protein synthesis during contractions

DEFF Research Database (Denmark)

Rose, Adam John; Alsted, Thomas Junker; Jensen, Thomas Elbenhardt

2009-01-01

Skeletal muscle protein synthesis rate decreases during contractions but the underlying regulatory mechanisms are poorly understood. It was hypothesised that there would be a coordinated regulation of eukaryotic elongation factor 2 (eEF2) and eukaryotic initiation factor 4E-binding protein 1 (4EBP1......) phosphorylation by signalling cascades downstream of rises in intracellular [Ca(2+)] and decreased energy charge via AMP activated protein kinase (AMPK) in contracting skeletal muscle. When fast-twitch skeletal muscles were contracted ex vivo using different protocols, the suppression of protein synthesis...... correlated more closely with changes in eEF2 rather than 4EBP1 phosphorylation. Using a combination of Ca(2+) release agents and ATPase inhibitors it was shown that the 60-70% suppression of fast-twitch skeletal muscle protein synthesis during contraction was equally distributed between Ca(2+) and energy...

Analysis of trends between solar wind velocity and energetic electron fluxes at geostationary orbit using the reverse arrangement test

Science.gov (United States)

Aryan, Homayon; Boynton, Richard J.; Walker, Simon N.

2013-02-01

A correlation between solar wind velocity (VSW) and energetic electron fluxes (EEF) at the geosynchronous orbit was first identified more than 30 years ago. However, recent studies have shown that the relation between VSW and EEF is considerably more complex than was previously suggested. The application of process identification technique to the evolution of electron fluxes in the range 1.8 - 3.5 MeV has also revealed peculiarities in the relation between VSW and EEF at the geosynchronous orbit. It has been revealed that for a constant solar wind density, EEF increase with VSW until a saturation velocity is reached. Beyond the saturation velocity, an increase in VSW is statistically not accompanied with EEF enhancement. The present study is devoted to the investigation of saturation velocity and its dependency upon solar wind density using the reverse arrangement test. In general, the results indicate that saturation velocity increases as solar wind density decreases. This implies that solar wind density plays an important role in defining the relationship between VSW and EEF at the geosynchronous orbit.

[Identification and analysis of the proteins interacted with Prestin in cochlear outer hair cells of guinea pig].

Science.gov (United States)

Luo, X; Wang, J Y; Zhang, F L; Xia, Y

2018-01-07

Objective: To explore the regulation and mechanism of Prestin protein by identifying the proteins interacted with Prestin in cochlear outer hair cell(OHC) and analyzing their biological function. Methods: Co-immunoprecipitation combined mass spectrometry technology was used to isolate and identify the proteins interacted with Prestin protein of OHC, bioinformatics was used to construct Prestin protein interaction network. The proteins interacted with Prestin in OHC of guinea pig were determined by matching primary interaction mass spectrometry with protein interaction network, and annotated their functions. Results: The results of co-immunoprecipitation combined with mass spectrometry showed that 116 kinds of credible proteins could interact with Prestin. By constructing Prestin protein interaction network, matching the results of mass spectrometry and analyzing of sub-cellular localization, eight kinds of proteins were confirmed that they interacted with Prestin directly, namely EEF2, HSP90AB1, FN1, FLNA, EEF1A1, HSP90B1, ATP5A1, and ERH, respectively, which were mainly involved in the synthesis and transportation, transmembrane folding and localization, structural stability and signal transduction of Prestin protein. Conclusion: EEF2, HSP90AB1, FN1, FLNA, EEF1A1, HSP90B1, ATP5A1 and ERH provide molecular basis for sensory amplification function of OHCs by participating in biotransformation, transmembrane folding and localization, signal transduction and other biological processes of Prestin protein.

Tier-1 and Tier-2 real-time analysis experience in CMS Data Challenge 2004

CERN Document Server

De Filippis, N; Pierro, A; Silvestris, L; Fanfani, A; Grandi, C; Hernández, J M; Bonacorsi, D; Corvo, M; Fanzago, F

2005-01-01

During the CMS Data Challenge 2004 a real-time analysis was attempted at INFN and PIC Tier-1 and Tier-2s in order to test the ability of the instrumented methods to quickly process the data. Several agents and automatic procedures were implemented to perform the analysis at the Tier-1/2 synchronously with the data transfer from Tier-0 at CERN. The system was implemented in the LCG-2 Grid environment and allowed on-the-fly job preparation and subsequent submission to the Resource Broker as new data came along. Running job accessed data from the Storage Elements via remote file protocol, whenever possible, or copying them locally with replica manager commands. Details of the procedures adopted to run the analysis jobs and the expected results are described. An evaluation of the ability of the system to maintain an analysis rate at Tier-1 and Tier-2 comparable with the data transfer rate is also presented. The results on the analysis timeline, the statistics of submitted jobs, the overall efficiency of the GRID ...

Understanding the Fate of Applied Nitrogen in Pine Plantations of the Southeastern United States Using 15N Enriched Fertilizers

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Jay E. Raymond

2016-11-01

Full Text Available This study was conducted to determine the efficacy of using enhanced efficiency fertilizer (EEFs products compared to urea to improve fertilizer nitrogen use efficiency (FNUE in forest plantations. All fertilizer treatments were labeled with 15N (0.5 atom percent and applied to 100 m2 circular plots at 12 loblolly pine stands (Pinus taeda L. across the southeastern United States. Total fertilizer N recovery for fertilizer treatments was determined by sampling all primary ecosystem components and using a mass balance calculation. Significantly more fertilizer N was recovered for all EEFs compared to urea, but there were generally no differences among EEFs. The total fertilizer N ecosystem recovery ranged from 81.9% to 84.2% for EEFs compared to 65.2% for urea. The largest amount of fertilizer N recovered for all treatments was in the loblolly pine trees (EEFs: 38.5%–49.9%, urea: 34.8% and soil (EEFs: 30.6%–38.8%, urea: 28.4%. This research indicates that a greater ecosystem fertilizer N recovery for EEFs compared to urea in southeastern pine plantations can potentially lead to increased FNUE in these systems.

A non-catalytic N-terminal domain negatively influences the nucleotide exchange activity of translation elongation factor 1Bα.

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Trosiuk, Tetiana V; Shalak, Vyacheslav F; Szczepanowski, Roman H; Negrutskii, Boris S; El'skaya, Anna V

2016-02-01

Eukaryotic translation elongation factor 1Bα (eEF1Bα) is a functional homolog of the bacterial factor EF-Ts, and is a component of the macromolecular eEF1B complex. eEF1Bα functions as a catalyst of guanine nucleotide exchange on translation elongation factor 1A (eEF1A). The C-terminal domain of eEF1Bα is necessary and sufficient for its catalytic activity, whereas the N-terminal domain interacts with eukaryotic translation elongation factor 1Bγ (eEF1Bγ) to form a tight complex. However, eEF1Bγ has been shown to enhance the catalytic activity of eEF1Bα attributed to the C-terminal domain of eEF1Bα. This suggests that the N-terminal domain of eEF1Bα may in some way influence the guanine nucleotide exchange process. We have shown that full-length recombinant eEF1Bα and its truncated forms are non-globular proteins with elongated shapes. Truncation of the N-terminal domain of eEF1Bα, which is dispensable for catalytic activity, resulted in acceleration of the rate of guanine nucleotide exchange on eEF1A compared to full-length eEF1Bα. A similar effect on the catalytic activity of eEF1Bα was observed after its interaction with eEF1Bγ. We suggest that the non-catalytic N-terminal domain of eEF1Bα may interfere with eEF1A binding to the C-terminal catalytic domain, resulting in a decrease in the overall rate of the guanine nucleotide exchange reaction. Formation of a tight complex between the eEF1Bγ and eEF1Bα N-terminal domains abolishes this inhibitory effect. © 2015 FEBS.

Performance of large electron energy filter in large volume plasma device

International Nuclear Information System (INIS)

Singh, S. K.; Srivastava, P. K.; Awasthi, L. M.; Mattoo, S. K.; Sanyasi, A. K.; Kaw, P. K.; Singh, R.

2014-01-01

This paper describes an in-house designed large Electron Energy Filter (EEF) utilized in the Large Volume Plasma Device (LVPD) [S. K. Mattoo, V. P. Anita, L. M. Awasthi, and G. Ravi, Rev. Sci. Instrum. 72, 3864 (2001)] to secure objectives of (a) removing the presence of remnant primary ionizing energetic electrons and the non-thermal electrons, (b) introducing a radial gradient in plasma electron temperature without greatly affecting the radial profile of plasma density, and (c) providing a control on the scale length of gradient in electron temperature. A set of 19 independent coils of EEF make a variable aspect ratio, rectangular solenoid producing a magnetic field (B x ) of 100 G along its axis and transverse to the ambient axial field (B z ∼ 6.2 G) of LVPD, when all its coils are used. Outside the EEF, magnetic field reduces rapidly to 1 G at a distance of 20 cm from the center of the solenoid on either side of target and source plasma. The EEF divides LVPD plasma into three distinct regions of source, EEF and target plasma. We report that the target plasma (n e ∼ 2 × 10 11  cm −3 and T e ∼ 2 eV) has no detectable energetic electrons and the radial gradients in its electron temperature can be established with scale length between 50 and 600 cm by controlling EEF magnetic field. Our observations reveal that the role of the EEF magnetic field is manifested by the energy dependence of transverse electron transport and enhanced transport caused by the plasma turbulence in the EEF plasma

Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.

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Thrush Anthony

2010-01-01

Full Text Available Abstract Background Perennial ryegrass (Lolium perenne L. is an important pasture and turf crop. Biotechniques such as gene expression studies are being employed to improve traits in this temperate grass. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR is among the best methods available for determining changes in gene expression. Before analysis of target gene expression, it is essential to select an appropriate normalisation strategy to control for non-specific variation between samples. Reference genes that have stable expression at different biological and physiological states can be effectively used for normalisation; however, their expression stability must be validated before use. Results Existing Serial Analysis of Gene Expression data were queried to identify six moderately expressed genes that had relatively stable gene expression throughout the year. These six candidate reference genes (eukaryotic elongation factor 1 alpha, eEF1A; TAT-binding protein homolog 1, TBP-1; eukaryotic translation initiation factor 4 alpha, eIF4A; YT521-B-like protein family protein, YT521-B; histone 3, H3; ubiquitin-conjugating enzyme, E2 were validated for qRT-PCR normalisation in 442 diverse perennial ryegrass (Lolium perenne L. samples sourced from field- and laboratory-grown plants under a wide range of experimental conditions. Eukaryotic EF1A is encoded by members of a multigene family exhibiting differential expression and necessitated the expression analysis of different eEF1A encoding genes; a highly expressed eEF1A (h, a moderately, but stably expressed eEF1A (s, and combined expression of multigene eEF1A (m. NormFinder identified eEF1A (s and YT521-B as the best combination of two genes for normalisation of gene expression data in perennial ryegrass following different defoliation management in the field. Conclusions This study is unique in the magnitude of samples tested with the inclusion of numerous field-grown samples

Eukaryotic elongation factor 2 controls TNF-α translation in LPS-induced hepatitis

Science.gov (United States)

González-Terán, Bárbara; Cortés, José R.; Manieri, Elisa; Matesanz, Nuria; Verdugo, ρngeles; Rodríguez, María E.; González-Rodríguez, ρgueda; Valverde, ρngela; Martín, Pilar; Davis, Roger J.; Sabio, Guadalupe

2012-01-01

Bacterial LPS (endotoxin) has been implicated in the pathogenesis of acute liver disease through its induction of the proinflammatory cytokine TNF-α. TNF-α is a key determinant of the outcome in a well-established mouse model of acute liver failure during septic shock. One possible mechanism for regulating TNF-α expression is through the control of protein elongation during translation, which would allow rapid cell adaptation to physiological changes. However, the regulation of translational elongation is poorly understood. We found that expression of p38γ/δ MAPK proteins is required for the elongation of nascent TNF-α protein in macrophages. The MKK3/6-p38γ/δ pathway mediated an inhibitory phosphorylation of eukaryotic elongation factor 2 (eEF2) kinase, which in turn promoted eEF2 activation (dephosphorylation) and subsequent TNF-α elongation. These results identify a new signaling pathway that regulates TNF-α production in LPS-induced liver damage and suggest potential cell-specific therapeutic targets for liver diseases in which TNF-α production is involved. PMID:23202732

Heat shock-induced accumulation of translation elongation and termination factors precedes assembly of stress granules in S. cerevisiae.

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Tomas Grousl

Full Text Available In response to severe environmental stresses eukaryotic cells shut down translation and accumulate components of the translational machinery in stress granules (SGs. Since they contain mainly mRNA, translation initiation factors and 40S ribosomal subunits, they have been referred to as dominant accumulations of stalled translation preinitiation complexes. Here we present evidence that the robust heat shock-induced SGs of S. cerevisiae also contain translation elongation factors eEF3 (Yef3p and eEF1Bγ2 (Tef4p as well as translation termination factors eRF1 (Sup45p and eRF3 (Sup35p. Despite the presence of the yeast prion protein Sup35 in heat shock-induced SGs, we found out that its prion-like domain is not involved in the SGs assembly. Factors eEF3, eEF1Bγ2 and eRF1 were accumulated and co-localized with Dcp2 foci even upon a milder heat shock at 42°C independently of P-bodies scaffolding proteins. We also show that eEF3 accumulations at 42°C determine sites of the genuine SGs assembly at 46°C. We suggest that identification of translation elongation and termination factors in SGs might help to understand the mechanism of the eIF2α factor phosphorylation-independent repression of translation and SGs assembly.

Challenges for Blog Analysis and Possible Solutions

Science.gov (United States)

Nguyen-Ngoc, Anh Vu; Law, Effie Lai-Chong

Blogs are increasingly used as an educational tool because of their ease of use for web-based publishing, enabling users to share their thoughts that invite intellectual and social discourses. Blogs are becoming a significant component of many web-based learning environments. However, there are 2 major challenges in applying blogs in web-based learning: (i) browsing/searching blog archives are technically not well supported in a learning environment in which several blogs are used by both facilitators and students; (ii) there is lack of a theoretically sound and usable blog analysis scheme to analyse blog content in order to support the evaluation of collaborative learning activities in web-based courses. In this paper, we describe some possible solutions for these challenges based on our research on web-based collaborative learning settings. Our experience of applying blogs in a web-based cross-cultural collaborative learning course is also reported.

Distributed analysis challenges in ATLAS

Energy Technology Data Exchange (ETDEWEB)

Duckeck, Guenter; Legger, Federica; Mitterer, Christoph Anton; Walker, Rodney [Ludwig-Maximilians-Universitaet Muenchen (Germany)

2016-07-01

The ATLAS computing model has undergone massive changes to meet the high luminosity challenge of the second run of the Large Hadron Collider (LHC) at CERN. The production system and distributed data management have been redesigned, a new data format and event model for analysis have been introduced, and common reduction and derivation frameworks have been developed. We report on the impact these changes have on the distributed analysis system, study the various patterns of grid usage for user analysis, focusing on the differences between the first and th e second LHC runs, and measure performances of user jobs.

Cerebrovascular reactivity (CVR) MRI with CO2 challenge: A technical review.

Science.gov (United States)

Liu, Peiying; B De Vis, Jill; Lu, Hanzhang

2018-03-21

Cerebrovascular reactivity (CVR) is an indicator of cerebrovascular reserve and provides important information about vascular health in a range of brain conditions and diseases. Unlike steady-state vascular parameters, such as cerebral blood flow (CBF) and cerebral blood volume (CBV), CVR measures the ability of cerebral vessels to dilate or constrict in response to challenges or maneuvers. Therefore, CVR mapping requires a physiological challenge while monitoring the corresponding hemodynamic changes in the brain. The present review primarily focuses on methods that use CO2 inhalation as a physiological challenge while monitoring changes in hemodynamic MRI signals. CO2 inhalation has been increasingly used in CVR mapping in recent literature due to its potency in causing vasodilation, rapid onset and cessation of the effect, as well as advances in MRI-compatible gas delivery apparatus. In this review, we first discuss the physiological basis of CVR mapping using CO2 inhalation. We then review the methodological aspects of CVR mapping, including gas delivery apparatus, the timing paradigm of the breathing challenge, the MRI imaging sequence, and data analysis. In addition, we review alternative approaches for CVR mapping that do not require CO2 inhalation. Copyright © 2018. Published by Elsevier Inc.

Semiconductor product analysis challenges based on the 1999 ITRS

International Nuclear Information System (INIS)

Joseph, Thomas W.; Anderson, Richard E.; Gilfeather, Glen; LeClaire, Carole; Yim, Daniel

2001-01-01

One of the most significant challenges for technology characterization and failure analysis is to keep instrumentation and techniques in step with the development of technology itself. Not only are dimensions shrinking and new materials being employed, but the rate of change is increasing. According to the 1999 International Technology Roadmap for Semiconductors, 'The number and difficulty of the technical challenges continue to increase as technology moves forward'. It could be argued that technology cannot be developed without appropriate analytical techniques; nevertheless while much effort is being directed at materials and processes, only a small proportion is being directed at analysis. Whereas previous versions of the Semiconductor Industry Association roadmap contained a small number of implicit references to characterization and analysis, the 1999 ITRS contains many explicit references. It is clear that characterization is now woven through the roadmap, and technology developers in all areas appreciate the fact that new instrumentation and techniques will be required to sustain the rate of development the semiconductor industry has seen in recent years. Late in 1999, a subcommittee of the Sematech Product Analysis Forum (PAF) reviewed the ITRS and identified a 'top-ten' list of challenges which the failure analysis community will face as present technologies are extended and future technologies are developed. This paper discusses the PAF top-ten list of challenges, which is based primarily on the Difficult Challenges tables from each ITRS working group. Eight of the top-ten are challenges of significant technical magnitude; only two could be considered non-technical in nature. Most of these challenges cut across several working group areas and could be considered common threads in the roadmap, ranging from fault simulation and modeling to imaging small features, from electrical defect isolation to deprocessing. While evolutionary changes can be anticipated

Challenges of Big Data Analysis.

Science.gov (United States)

Fan, Jianqing; Han, Fang; Liu, Han

2014-06-01

Big Data bring new opportunities to modern society and challenges to data scientists. On one hand, Big Data hold great promises for discovering subtle population patterns and heterogeneities that are not possible with small-scale data. On the other hand, the massive sample size and high dimensionality of Big Data introduce unique computational and statistical challenges, including scalability and storage bottleneck, noise accumulation, spurious correlation, incidental endogeneity, and measurement errors. These challenges are distinguished and require new computational and statistical paradigm. This article gives overviews on the salient features of Big Data and how these features impact on paradigm change on statistical and computational methods as well as computing architectures. We also provide various new perspectives on the Big Data analysis and computation. In particular, we emphasize on the viability of the sparsest solution in high-confidence set and point out that exogeneous assumptions in most statistical methods for Big Data can not be validated due to incidental endogeneity. They can lead to wrong statistical inferences and consequently wrong scientific conclusions.

Challenges on innovations of newly-developed safety analysis codes

International Nuclear Information System (INIS)

Yang, Yanhua; Zhang, Hao

2016-01-01

With the development of safety analysis method, the safety analysis codes meet more challenges. Three challenges are presented in this paper, which are mathematic model, code design and user interface. Combined with the self-reliance safety analysis code named COSINE, the ways of meeting these requirements are suggested, that is to develop multi-phases, multi-fields and multi-dimension models, to adopt object-oriented code design ideal and to improve the way of modeling, calculation control and data post-processing in the user interface.

Challenges on innovations of newly-developed safety analysis codes

Energy Technology Data Exchange (ETDEWEB)

Yang, Yanhua [Shanghai Jiao Tong Univ. (China). School of Nuclear Science and Engineering; Zhang, Hao [State Nuclear Power Software Development Center, Beijing (China). Beijing Future Science and Technology City

2016-05-15

With the development of safety analysis method, the safety analysis codes meet more challenges. Three challenges are presented in this paper, which are mathematic model, code design and user interface. Combined with the self-reliance safety analysis code named COSINE, the ways of meeting these requirements are suggested, that is to develop multi-phases, multi-fields and multi-dimension models, to adopt object-oriented code design ideal and to improve the way of modeling, calculation control and data post-processing in the user interface.

Eukaryotic translation initiator protein 1A isoform, CCS-3, enhances the transcriptional repression of p21CIP1 by proto-oncogene FBI-1 (Pokemon/ZBTB7A).

Science.gov (United States)

Choi, Won-Il; Kim, Youngsoo; Kim, Yuri; Yu, Mi-young; Park, Jungeun; Lee, Choong-Eun; Jeon, Bu-Nam; Koh, Dong-In; Hur, Man-Wook

2009-01-01

FBI-1, a member of the POK (POZ and Kruppel) family of transcription factors, plays a role in differentiation, oncogenesis, and adipogenesis. eEF1A is a eukaryotic translation elongation factor involved in several cellular processes including embryogenesis, oncogenic transformation, cell proliferation, and cytoskeletal organization. CCS-3, a potential cervical cancer suppressor, is an isoform of eEF1A. We found that eEF1A forms a complex with FBI-1 by co-immunoprecipitation, SDS-PAGE, and MALDI-TOF Mass analysis of the immunoprecipitate. GST fusion protein pull-downs showed that FBI-1 directly interacts with eEF1A and CCS-3 via the zinc finger and POZ-domain of FBI-1. FBI-1 co-localizes with either eEF1A or CCS-3 at the nuclear periplasm. CCS-3 enhances transcriptional repression of the p21CIP1 gene (hereafter referred to as p21) by FBI-1. The POZ-domain of FBI-1 interacts with the co-repressors, SMRT and BCoR. We found that CCS-3 also interacts with the co-repressors independently. The molecular interaction between the co-repressors and CCS-3 at the POZ-domain of FBI-1 appears to enhance FBI-1 mediated transcriptional repression. Our data suggest that CCS-3 may be important in cell differentiation, tumorigenesis, and oncogenesis by interacting with the proto-oncogene FBI-1 and transcriptional co-repressors. Copyright 2009 S. Karger AG, Basel.

Facility for generating crew waste water product for ECLSS testing

Science.gov (United States)

Buitekant, Alan; Roberts, Barry C.

1990-01-01

An End-use Equipment Facility (EEF) has been constructed which is used to simulate water interfaces between the Space Station Freedom Environmental Control and Life Support Systems (ECLSS) and man systems. The EEF is used to generate waste water to be treated by ECLSS water recovery systems. The EEF will also be used to close the water recovery loop by allowing test subjects to use recovered hygiene and potable water during several phases of testing. This paper describes the design and basic operation of the EEF.

Plasma response to electron energy filter in large volume plasma device

International Nuclear Information System (INIS)

Sanyasi, A. K.; Awasthi, L. M.; Mattoo, S. K.; Srivastava, P. K.; Singh, S. K.; Singh, R.; Kaw, P. K.

2013-01-01

An electron energy filter (EEF) is embedded in the Large Volume Plasma Device plasma for carrying out studies on excitation of plasma turbulence by a gradient in electron temperature (ETG) described in the paper of Mattoo et al. [S. K. Mattoo et al., Phys. Rev. Lett. 108, 255007 (2012)]. In this paper, we report results on the response of the plasma to the EEF. It is shown that inhomogeneity in the magnetic field of the EEF switches on several physical phenomena resulting in plasma regions with different characteristics, including a plasma region free from energetic electrons, suitable for the study of ETG turbulence. Specifically, we report that localized structures of plasma density, potential, electron temperature, and plasma turbulence are excited in the EEF plasma. It is shown that structures of electron temperature and potential are created due to energy dependence of the electron transport in the filter region. On the other hand, although structure of plasma density has origin in the particle transport but two distinct steps of the density structure emerge from dominance of collisionality in the source-EEF region and of the Bohm diffusion in the EEF-target region. It is argued and experimental evidence is provided for existence of drift like flute Rayleigh-Taylor in the EEF plasma

WFIRST: Microlensing Analysis Data Challenge

Science.gov (United States)

Street, Rachel; WFIRST Microlensing Science Investigation Team

2018-01-01

WFIRST will produce thousands of high cadence, high photometric precision lightcurves of microlensing events, from which a wealth of planetary and stellar systems will be discovered. However, the analysis of such lightcurves has historically been very time consuming and expensive in both labor and computing facilities. This poses a potential bottleneck to deriving the full science potential of the WFIRST mission. To address this problem, the WFIRST Microlensing Science Investigation Team designing a series of data challenges to stimulate research to address outstanding problems of microlensing analysis. These range from the classification and modeling of triple lens events to methods to efficiently yet thoroughly search a high-dimensional parameter space for the best fitting models.

Compound-specific radiocarbon analysis - Analytical challenges and applications

Science.gov (United States)

Mollenhauer, G.; Rethemeyer, J.

2009-01-01

Within the last decades, techniques have become available that allow measurement of isotopic compositions of individual organic compounds (compound-specific isotope measurements). Most often the carbon isotopic composition of these compounds is studied, including stable carbon (δ13C) and radiocarbon (Δ14C) measurements. While compound-specific stable carbon isotope measurements are fairly simple, and well-established techniques are widely available, radiocarbon analysis of specific organic compounds is a more challenging method. Analytical challenges include difficulty obtaining adequate quantities of sample, tedious and complicated laboratory separations, the lack of authentic standards for measuring realistic processing blanks, and large uncertainties in values of Δ14C at small sample sizes. The challenges associated with sample preparation for compound-specific Δ14C measurements will be discussed in this contribution. Several years of compound-specific radiocarbon analysis have revealed that in most natural samples, purified organic compounds consist of heterogeneous mixtures of the same compound. These mixtures could derive from multiple sources, each having a different initial reservoir age but mixed in the same terminal reservoir, from a single source but mixed after deposition, or from a prokaryotic organism using variable carbon sources including mobilization of ancient carbon. These processes not only represent challenges to the interpretation of compound-specific radiocarbon data, but provide unique tools for the understanding of biogeochemical and sedimentological processes influencing the preserved organic geochemical records in marine sediments. We will discuss some examples where compound-specific radiocarbon analysis has provided new insights for the understanding of carbon source utilization and carbon cycling.

Cultural and family challenges to managing type 2 diabetes in immigrant Chinese Americans.

Science.gov (United States)

Chesla, Catherine A; Chun, Kevin M; Kwan, Christine M L

2009-10-01

Although Asians demonstrate elevated levels of type 2 diabetes, little attention has been directed to their unique cultural beliefs and practices regarding diabetes. We describe cultural and family challenges to illness management in foreign-born Chinese American patients with type 2 diabetes and their spouses. This was an interpretive comparative interview study with 20 foreign-born Chinese American couples (n = 40) living with type 2 diabetes. Multiple (six to seven) semistructured interviews with each couple in individual, group, and couple settings elicited beliefs about diabetes and narratives of care within the family and community. Interpretive narrative and thematic analysis were completed. A separate respondent group of 19 patients and spouses who met the inclusion criteria reviewed and confirmed the themes developed from the initial couples. Cultural and family challenges to diabetes management within foreign-born Chinese American families included how 1) diabetes symptoms challenged family harmony, 2) dietary prescriptions challenged food beliefs and practices, and 3) disease management requirements challenged established family role responsibilities. Culturally nuanced care with immigrant Chinese Americans requires attentiveness to the social context of disease management. Patients' and families' disease management decisions are seldom made independent of their concerns for family well-being, family face, and the reciprocal responsibilities required by varied family roles. Framing disease recommendations to include cultural concerns for balance and significant food rituals are warranted.

Methodological challenges in qualitative content analysis: A discussion paper.

Science.gov (United States)

Graneheim, Ulla H; Lindgren, Britt-Marie; Lundman, Berit

2017-09-01

This discussion paper is aimed to map content analysis in the qualitative paradigm and explore common methodological challenges. We discuss phenomenological descriptions of manifest content and hermeneutical interpretations of latent content. We demonstrate inductive, deductive, and abductive approaches to qualitative content analysis, and elaborate on the level of abstraction and degree of interpretation used in constructing categories, descriptive themes, and themes of meaning. With increased abstraction and interpretation comes an increased challenge to demonstrate the credibility and authenticity of the analysis. A key issue is to show the logic in how categories and themes are abstracted, interpreted, and connected to the aim and to each other. Qualitative content analysis is an autonomous method and can be used at varying levels of abstraction and interpretation. Copyright © 2017 Elsevier Ltd. All rights reserved.

Metabolomic Analysis in Brain Research: Opportunities & Challenges

Directory of Open Access Journals (Sweden)

Catherine G Vasilopoulou

2016-05-01

Full Text Available Metabolism being a fundamental part of molecular physiology, elucidating the structure and regulation of metabolic pathways is crucial for obtaining a comprehensive perspective of cellular function and understanding the underlying mechanisms of its dysfunction(s. Therefore, quantifying an accurate metabolic network activity map under various physiological conditions is among the major objectives of systems biology in the context of many biological applications. Especially for CNS, metabolic network activity analysis can substantially enhance our knowledge about the complex structure of the mammalian brain and the mechanisms of neurological disorders, leading to the design of effective therapeutic treatments. Metabolomics has emerged as the high-throughput quantitative analysis of the concentration profile of small molecular weight metabolites, which act as reactants and products in metabolic reactions and as regulatory molecules of proteins participating in many biological processes. Thus, the metabolic profile provides a metabolic activity fingerprint, through the simultaneous analysis of tens to hundreds of molecules of pathophysiological and pharmacological interest. The application of metabolomics is at its standardization phase in general, and the challenges for paving a standardized procedure are even more pronounced in brain studies. In this review, we support the value of metabolomics in brain research. Moreover, we demonstrate the challenges of designing and setting up a reliable brain metabolomic study, which, among other parameters, has to take into consideration the sex differentiation and the complexity of brain physiology manifested in its regional variation. We finally propose ways to overcome these challenges and design a study that produces reproducible and consistent results.

Meta-analysis of the performance variation in broilers experimentally challenged by Eimeria spp.

Science.gov (United States)

Kipper, Marcos; Andretta, Ines; Lehnen, Cheila Roberta; Lovatto, Paulo Alberto; Monteiro, Silvia Gonzalez

2013-09-01

A meta-analysis was carried out to (1) study the relation of the variation in feed intake and weight gain in broilers infected with Eimeria acervulina, Eimeria maxima, Eimeria tenella, or a Pool of Eimeria species, and (2) to identify and to quantify the effects involved in the infection. A database of articles addressing the experimental infection with Coccidia in broilers was developed. These publications must present results of animal performance (weight gain, feed intake, and feed conversion ratio). The database was composed by 69 publications, totalling around 44 thousand animals. Meta-analysis followed three sequential analyses: graphical, correlation, and variance-covariance. The feed intake of the groups challenged by E. acervulina and E. tenella did not differ (P>0.05) to the control group. However, the feed intake in groups challenged by E. maxima and Pool showed an increase of 8% and 5% (PEimeria species, animal age, sex, and genetic line. In general the age effect is superior to the challenge effect, showing that age at the challenge is important to determine the impact of Eimeria infection. Copyright © 2013 Elsevier B.V. All rights reserved.

IMAGE ANALYSIS FOR COSMOLOGY: RESULTS FROM THE GREAT10 STAR CHALLENGE

International Nuclear Information System (INIS)

Kitching, T. D.; Heymans, C.; Rowe, B.; Witherick, D.; Gill, M.; Massey, R.; Courbin, F.; Gentile, M.; Meylan, G.; Georgatzis, K.; Gruen, D.; Kilbinger, M.; Li, G. L.; Mariglis, A. P.; Storkey, A.; Xin, B.

2013-01-01

We present the results from the first public blind point-spread function (PSF) reconstruction challenge, the GRavitational lEnsing Accuracy Testing 2010 (GREAT10) Star Challenge. Reconstruction of a spatially varying PSF, sparsely sampled by stars, at non-star positions is a critical part in the image analysis for weak lensing where inaccuracies in the modeled ellipticity e and size R 2 can impact the ability to measure the shapes of galaxies. This is of importance because weak lensing is a particularly sensitive probe of dark energy and can be used to map the mass distribution of large scale structure. Participants in the challenge were presented with 27,500 stars over 1300 images subdivided into 26 sets, where in each set a category change was made in the type or spatial variation of the PSF. Thirty submissions were made by nine teams. The best methods reconstructed the PSF with an accuracy of σ(e) ≈ 2.5 × 10 –4 and σ(R 2 )/R 2 ≈ 7.4 × 10 –4 . For a fixed pixel scale, narrower PSFs were found to be more difficult to model than larger PSFs, and the PSF reconstruction was severely degraded with the inclusion of an atmospheric turbulence model (although this result is likely to be a strong function of the amplitude of the turbulence power spectrum).

Re-challenge Studies in Non-celiac Gluten Sensitivity: A Systematic Review and Meta-Analysis

Directory of Open Access Journals (Sweden)

Elena Lionetti

2017-09-01

Full Text Available Background: Non-celiac gluten sensitivity (NCGS is a clinical entity characterized by intestinal and/or extra-intestinal symptoms related to the ingestion of gluten in individuals that are not affected by either celiac disease (CD or wheat allergy (WA. Since we do not have specific biomarkers for NCGS, the diagnosis is based on the evidence of a clear relationship between the ingestion of gluten (re-challenge and clinical symptoms, after a remission during the gluten-free diet (GFD. Several re-challenge studies have been published so far to evaluate the real prevalence of NCGS, reporting conflicting results. In the present article, we provide a systematic review with meta-analysis of the existing literature on re-challenge studies to evaluate prevalence figures of NCGS after re-challenge procedures.Methods: All clinical trials performing a gluten re-challenge with or without a placebo control in patients with a suspected diagnosis of NCGS were included. Search results were limited to studies published in English language. No publication date or publication status restrictions were imposed.Results: Eleven studies were included in the meta-analysis. There was a considerable heterogeneity related to different sample size, type, and amount of gluten administered, duration of challenge and different type of placebo. The overall pooled percentage of patients with a diagnosis of NCGS relapsing after a gluten challenge was 30%, ranging between 7 and 77%. The meta-analysis showed a not significant relative risk (RR of relapse after gluten challenge as compared to placebo (RR = 0.4; 95% CI = −0.15–0.9; p = 0.16. The overall pooled percentage of patients with a diagnosis of NCGS relapsing after a gluten challenge performed according to the recent Salerno criteria was significantly higher as compared to the percentage of patients relapsing after placebo (40 vs. 24%; p = 0.003, with a significant RR of relapse after gluten challenge as compared to

Challenges in Resolution for IC Failure Analysis

Science.gov (United States)

Martinez, Nick

1999-10-01

Resolution is becoming more and more of a challenge in the world of Failure Analysis in integrated circuits. This is a result of the ongoing size reduction in microelectronics. Determining the cause of a failure depends upon being able to find the responsible defect. The time it takes to locate a given defect is extremely important so that proper corrective actions can be taken. The limits of current microscopy tools are being pushed. With sub-micron feature sizes and even smaller killing defects, optical microscopes are becoming obsolete. With scanning electron microscopy (SEM), the resolution is high but the voltage involved can make these small defects transparent due to the large mean-free path of incident electrons. In this presentation, I will give an overview of the use of inspection methods in Failure Analysis and show example studies of my work as an Intern student at Texas Instruments. 1. Work at Texas Instruments, Stafford, TX, was supported by TI. 2. Work at Texas Tech University, was supported by NSF Grant DMR9705498.

IMAGE ANALYSIS FOR COSMOLOGY: RESULTS FROM THE GREAT10 STAR CHALLENGE

Energy Technology Data Exchange (ETDEWEB)

Kitching, T. D.; Heymans, C. [Mullard Space Science Laboratory, University College London, Holmbury St. Mary, Dorking, Surrey RH5 6NT (United Kingdom); Rowe, B.; Witherick, D. [Department of Physics and Astronomy, University College London, Gower Street, London WC1E 6BT (United Kingdom); Gill, M. [Center for Cosmology and AstroParticle Physics, Physics Department, The Ohio State University, Columbus, OH (United States); Massey, R. [Institute for Computational Cosmology, Durham University, South Road, Durham DH1 3LE (United Kingdom); Courbin, F.; Gentile, M.; Meylan, G. [Laboratoire d' Astrophysique, Ecole Polytechnique Federale de Lausanne (EPFL) (Switzerland); Georgatzis, K. [Department of Information and Computer Science, Aalto University, P.O. Box 15400, FI-00076 Aalto (Finland); Gruen, D. [Department of Physics and Astronomy, 209 South 33rd Street, University of Pennsylvania, Philadelphia, PA 19104 (United States); Kilbinger, M. [Excellence Cluster Universe, Boltzmannstr. 2, D-85748 Garching (Germany); Li, G. L. [Purple Mountain Observatory, 2 West Beijing Road, Nanjing 210008 (China); Mariglis, A. P.; Storkey, A. [School of Informatics, University of Edinburgh, 10 Crichton Street, Edinburgh EH8 9AB (United Kingdom); Xin, B., E-mail: t.kitching@ucl.ac.uk [Department of Physics, Purdue University, 525 Northwestern Avenue, West Lafayette, IN 47907 (United States)

2013-04-01

We present the results from the first public blind point-spread function (PSF) reconstruction challenge, the GRavitational lEnsing Accuracy Testing 2010 (GREAT10) Star Challenge. Reconstruction of a spatially varying PSF, sparsely sampled by stars, at non-star positions is a critical part in the image analysis for weak lensing where inaccuracies in the modeled ellipticity e and size R {sup 2} can impact the ability to measure the shapes of galaxies. This is of importance because weak lensing is a particularly sensitive probe of dark energy and can be used to map the mass distribution of large scale structure. Participants in the challenge were presented with 27,500 stars over 1300 images subdivided into 26 sets, where in each set a category change was made in the type or spatial variation of the PSF. Thirty submissions were made by nine teams. The best methods reconstructed the PSF with an accuracy of {sigma}(e) Almost-Equal-To 2.5 Multiplication-Sign 10{sup -4} and {sigma}(R {sup 2})/R {sup 2} Almost-Equal-To 7.4 Multiplication-Sign 10{sup -4}. For a fixed pixel scale, narrower PSFs were found to be more difficult to model than larger PSFs, and the PSF reconstruction was severely degraded with the inclusion of an atmospheric turbulence model (although this result is likely to be a strong function of the amplitude of the turbulence power spectrum).

Challenges in the vulnerability and risk analysis of critical infrastructures

International Nuclear Information System (INIS)

Zio, Enrico

2016-01-01

The objective of this paper is to provide a systematic view on the problem of vulnerability and risk analysis of critical infrastructures. Reflections are made on the inherent complexities of these systems, related challenges are identified and possible ways forward for their analysis and management are indicated. Specifically: the framework of vulnerability and risk analysis is examined in relation to its application for the protection and resilience of critical infrastructures; it is argued that the complexity of these systems is a challenging characteristic, which calls for the integration of different modeling perspectives and new approaches of analysis; examples of are given in relation to the Internet and, particularly, the electric power grid, as representative of critical infrastructures and the associated complexity; the integration of different types of analyses and methods of system modeling is put forward for capturing the inherent structural and dynamic complexities of critical infrastructures and eventually evaluating their vulnerability and risk characteristics, so that decisions on protections and resilience actions can be taken with the required confidence. - Highlights: • The problem of the protection and resilience of CIs is the focus of the work. • The vulnerability and risk analysis framework for this is critically examined. • The complexity of CIs is presented as a challenge for system modeling and analysis. • The integration of different modeling perspectives of analysis is put forward as a solution. • The extension of the analysis framework to new methods for dealing with surprises and black swans is advocated.

Functional analysis-based interventions for challenging behaviour in dementia.

Science.gov (United States)

Moniz Cook, Esme D; Swift, Katie; James, Ian; Malouf, Reem; De Vugt, Marjolein; Verhey, Frans

2012-02-15

Functional analysis (FA) for the management of challenging behaviour is a promising behavioural intervention that involves exploring the meaning or purpose of an individual's behaviour. It extends the 'ABC' approach of behavioural analysis, to overcome the restriction of having to derive a single explanatory hypothesis for the person's behaviour. It is seen as a first line alternative to traditional pharmacological management for agitation and aggression. FA typically requires the therapist to develop and evaluate hypotheses-driven strategies that aid family and staff caregivers to reduce or resolve a person's distress and its associated behavioural manifestations. To assess the effects of functional analysis-based interventions for people with dementia (and their caregivers) living in their own home or in other settings. We searched ALOIS: the Cochrane Dementia and Cognitive Improvement Group's Specialized Register on 3 March 2011 using the terms: FA, behaviour (intervention, management, modification), BPSD, psychosocial and Dementia. Randomised controlled trials (RCTs) with reported behavioural outcomes that could be associated with functional analysis for the management of challenging behaviour in dementia. Four reviewers selected trials for inclusion. Two reviewers worked independently to extract data and assess trial quality, including bias. Meta-analyses for reported incidence, frequency, severity of care recipient challenging behaviour and mood (primary outcomes) and caregiver reaction, burden and mood were performed. Details of adverse effects were noted. Eighteen trials are included in the review. The majority were in family care settings. For fourteen studies, FA was just one aspect of a broad multi-component programme of care. Assessing the effect of FA was compromised by ill-defined protocols for the duration of component parts of these programmes (i.e. frequency of the intervention or actual time spent). Therefore, establishing the real effect of the

CMS Computing Software and Analysis Challenge 2006

Energy Technology Data Exchange (ETDEWEB)

De Filippis, N. [Dipartimento interateneo di Fisica M. Merlin and INFN Bari, Via Amendola 173, 70126 Bari (Italy)

2007-10-15

The CMS (Compact Muon Solenoid) collaboration is making a big effort to test the workflow and the dataflow associated with the data handling model. With this purpose the computing, software and analysis Challenge 2006, namely CSA06, started the 15th of September. It was a 50 million event exercise that included all the steps of the analysis chain, like the prompt reconstruction, the data streaming, calibration and alignment iterative executions, the data distribution to regional sites, up to the end-user analysis. Grid tools provided by the LCG project are also experimented to gain access to the data and the resources by providing a user friendly interface to the physicists submitting the production and the analysis jobs. An overview of the status and results of the CSA06 is presented in this work.

CMS Computing Software and Analysis Challenge 2006

International Nuclear Information System (INIS)

De Filippis, N.

2007-01-01

The CMS (Compact Muon Solenoid) collaboration is making a big effort to test the workflow and the dataflow associated with the data handling model. With this purpose the computing, software and analysis Challenge 2006, namely CSA06, started the 15th of September. It was a 50 million event exercise that included all the steps of the analysis chain, like the prompt reconstruction, the data streaming, calibration and alignment iterative executions, the data distribution to regional sites, up to the end-user analysis. Grid tools provided by the LCG project are also experimented to gain access to the data and the resources by providing a user friendly interface to the physicists submitting the production and the analysis jobs. An overview of the status and results of the CSA06 is presented in this work

The influence of future-oriented technology analysis: addressing the Cassandra challenge

OpenAIRE

JOHNSTON Ron; CAGNIN Cristiano

2011-01-01

This paper is based on a series of interviews with nine leading researchers conducted during the Future-Oriented Technology Analysis International Conference held in Seville on 16–17 October 2008. Analysis of these interviews paints a picture of FTA as an increasingly important approach being adopted in many countries to address the many challenges which are emerging at this time in human history. From this are drawn implications for the community of FTA practitioners. The biggest challenge i...

Challenges in combining different data sets during analysis when using grounded theory.

Science.gov (United States)

Rintala, Tuula-Maria; Paavilainen, Eija; Astedt-Kurki, Päivi

2014-05-01

To describe the challenges in combining two data sets during grounded theory analysis. The use of grounded theory in nursing research is common. It is a suitable method for studying human action and interaction. It is recommended that many alternative sources of data are collected to create as rich a dataset as possible. Data from interviews with people with diabetes (n=19) and their family members (n=19). Combining two data sets. When using grounded theory, there are numerous challenges in collecting and managing data, especially for the novice researcher. One challenge is to combine different data sets during the analysis. There are many methodological textbooks about grounded theory but there is little written in the literature about combining different data sets. Discussion is needed on the management of data and the challenges of grounded theory. This article provides a means for combining different data sets in the grounded theory analysis process.

Stability of Reference Genes for Messenger RNA Quantification by Real-Time PCR in Mouse Dextran Sodium Sulfate Experimental Colitis.

Directory of Open Access Journals (Sweden)

Nour Eissa

Full Text Available Many animal models have been developed to characterize the complexity of colonic inflammation. In dextran sodium sulfate (DSS experimental colitis in mice the choice of reference genes is critical for accurate quantification of target genes using quantitative real time PCR (RT-qPCR. No studies have addressed the performance of reference genes in mice DSS-experimental colitis. This study aimed to determine the stability of reference genes expression (RGE in DSS-experimental murine colitis.Colitis was induced in male C57BL/6 mice using DSS5% for 5 days, control group received water. RNA was extracted from inflamed and non-inflamed colon. Using RT-qPCR, comparative analysis of 13 RGE was performed according to predefined criteria and relative colonic TNF-α and IL-1β gene expression was determined by calculating the difference in the threshold cycle.Colitis significantly altered the stability of mucosal RGE. Commonly used glyceraldehyde-3-phosphate dehydrogenase (Gapdh, β-actin (Actb, or β2-microglobulin (β2m showed the highest variability within the inflamed and control groups. Conversely, TATA-box-binding protein (Tbp and eukaryotic translation elongation factor 2 (Eef2 were not affected by inflammation and were the most stable genes. Normalization of colonic TNF-α and IL-1β mRNA levels was dependent on the reference gene used. Depending on the genes used to normalize the data, statistical significance varied from significant when TBP / Eef2 were used to non-significant when Gapdh, Actb or β2m were used.This study highlights the appropriate choice of RGE to ensure adequate normalization of RT-qPCR data when using this model. Suboptimal RGE may explain controversial results from published studies. We recommend using Tbp and Eef2 instead of Gapdh, Actb or β2m as reference genes.

Next-Generation Climate Modeling Science Challenges for Simulation, Workflow and Analysis Systems

Science.gov (United States)

Koch, D. M.; Anantharaj, V. G.; Bader, D. C.; Krishnan, H.; Leung, L. R.; Ringler, T.; Taylor, M.; Wehner, M. F.; Williams, D. N.

2016-12-01

We will present two examples of current and future high-resolution climate-modeling research that are challenging existing simulation run-time I/O, model-data movement, storage and publishing, and analysis. In each case, we will consider lessons learned as current workflow systems are broken by these large-data science challenges, as well as strategies to repair or rebuild the systems. First we consider the science and workflow challenges to be posed by the CMIP6 multi-model HighResMIP, involving around a dozen modeling groups performing quarter-degree simulations, in 3-member ensembles for 100 years, with high-frequency (1-6 hourly) diagnostics, which is expected to generate over 4PB of data. An example of science derived from these experiments will be to study how resolution affects the ability of models to capture extreme-events such as hurricanes or atmospheric rivers. Expected methods to transfer (using parallel Globus) and analyze (using parallel "TECA" software tools) HighResMIP data for such feature-tracking by the DOE CASCADE project will be presented. A second example will be from the Accelerated Climate Modeling for Energy (ACME) project, which is currently addressing challenges involving multiple century-scale coupled high resolution (quarter-degree) climate simulations on DOE Leadership Class computers. ACME is anticipating production of over 5PB of data during the next 2 years of simulations, in order to investigate the drivers of water cycle changes, sea-level-rise, and carbon cycle evolution. The ACME workflow, from simulation to data transfer, storage, analysis and publication will be presented. Current and planned methods to accelerate the workflow, including implementing run-time diagnostics, and implementing server-side analysis to avoid moving large datasets will be presented.

Reduction of porcine circovirus type 2 (PCV2 viremia by a reformulated inactivated chimeric PCV1-2 vaccine-induced humoral and cellular immunity after experimental PCV2 challenge

Directory of Open Access Journals (Sweden)

Seo Hwi

2012-10-01

Full Text Available Abstract Background The objective of the present study was to elucidate the humoral and cellular immune response mechanisms by which a reformulated inactivated chimeric PCV1-2 vaccine reduces the PCV2 viremia. Forty PCV2 seronegative 3-week-old pigs were randomly divided into the following four groups: vaccinated challenged (T01, vaccinated non-challenged (T02, non-vaccinated challenged (T03, and non-vaccinated non-challenged (T04 animals. The pigs in groups T01 and T02 were immunized with a reformulated inactivated chimeric PCV1-2 vaccine (Fostera™ PCV; Pfizer Animal Health administered as a 2.0 ml dose at 21 days of age. At 35 days of age (0 days post-challenge, the pigs in groups T01 and T03 were inoculated intranasally with 2 ml each of PCV2b. Results A reduction of PCV2 viremia coincided with the appearance of both PCV2-specific neutralizing antibodies (NA and interferon-γ-secreting cells (IFN-γ-SCs in the vaccinated animals. However, the presence of anti-PCV2 IgG antibodies did not correlate with the reduction of PCV2 viremia. Lymphocyte subset analysis indicated that the numbers of CD3+ and CD4+ cells increased in vaccinated animals but the numbers of CD4+ cells decreased transiently in non-vaccinated animals. The observation of a delayed type hypersensitivity response in only the vaccinated animals also supports a CD4+ cell-associated protective cellular immune response induced by the reformulated inactivated chimeric PCV1-2 vaccine. Conclusions The induction of PCV2-specific NA and IFN-γ-SCs, and CD4+ cells by the reformulated inactivated chimeric PCV1-2 vaccine is the important protective immune response leading to reduction of the PCV2 viremia and control of the PCV2 infection. To our knowledge this is the first demonstration of protective humoral and cellular immunity induced by the reformulated inactivated chimeric PCV1-2 vaccine and its effect on reduction of PCV2 viremia by vaccination.

Exercising CMS dataflows and workflows in computing challenges at the SpanishTier-1 and Tier-2 sites

International Nuclear Information System (INIS)

Caballero, J; Colino, N; Peris, A D; G-Abia, P; Hernandez, J M; R-Calonge, F J; Cabrillo, I; Caballero, I G; Marco, R; Matorras, F; Flix, J; Merino, G

2008-01-01

An overview of the data transfer, processing and analysis operations conducted at the Spanish Tier-1 (PIC, Barcelona) and Tier-2 (CIEMAT-Madrid and IFCA-Santander federation) centres during the past CMS CSA06 Computing, Software and Analysis challenge and in preparation for CSA07 is presented

Tracking and disrupting dark networks: challenges of data collection and analysis

OpenAIRE

Roberts, Nancy C.

2011-01-01

The article of record as published may be found at http://dx.doi.org/10.1007/s10796-010-9271-z The attack on September 11, 2001 set off numerous efforts to counter terrorism and insurgencies. Central to these efforts has been the drive to improve data collection and analysis. Section 1 summarizes some of the more notable improvements among U.S. government agencies as they strive to develop their capabilities. Although progress has been made, daunting challenges remain. Section 2 reviews th...

PHENOLIC DERIVATIVES AND ANTIOXIDANT ACTIVITY OF POLAR EXTRACTS FROM Bauhinia pulchella

Directory of Open Access Journals (Sweden)

Adonias Almeida Carvalho

Full Text Available A tea made with the leaves or stem bark of Bauhinia pulchella Benth (Fabaceae is used in the treatment of diabetes. Ethanol (EEF and aqueous (EAF extracts were obtained from the leaves and the antioxidant and citotoxic activities were tested, as well as quantify the content of flavonoids and phenolic compounds (TPC. EEF and EAF showed similar profiles by HPLC, with the presence of three compounds. Column chromatography reverse phase (C18 and Sephadex LH-20, followed by semi preparative HPLC-C18 of EAF resulted in three flavonoids. Their structures were identified by 1H and 13C NMR as myricitrin (1, quercitrin (2 and afzelin (3. In assay of determination of reactive substances to thiobarbituric acid (TBARS, with DPPH, ABTS and nitric oxide (NO• free radicals, EAF showed antioxidant potential higher than the EEF. This is the first report of the occurrence of the flavonoids 1-3, in the species B. pulchella. EEF and EAF were inactive in the cytotoxicity assays. In short, the polar extracts from the leaves of B. pulchella proved to be promising sources of biomolecules phenolic, with antioxidant potential, which may, in the future, be used as chemical markers for species and validation of therapeutic use.

Analyzing Social Interactions: Promises and Challenges of Cross Recurrence Quantification Analysis

DEFF Research Database (Denmark)

Fusaroli, Riccardo; Konvalinka, Ivana; Wallot, Sebastian

2014-01-01

The scientific investigation of social interactions presents substantial challenges: interacting agents engage each other at many different levels and timescales (motor and physiological coordination, joint attention, linguistic exchanges, etc.), often making their behaviors interdependent in non......-linear ways. In this paper we review the current use of Cross Recurrence Quantification Analysis (CRQA) in the analysis of social interactions, and assess its potential and challenges. We argue that the method can sensitively grasp the dynamics of human interactions, and that it has started producing valuable...

Exercising CMS dataflows and workflows in computing challenges at the SpanishTier-1 and Tier-2 sites

Energy Technology Data Exchange (ETDEWEB)

Caballero, J; Colino, N; Peris, A D; G-Abia, P; Hernandez, J M; R-Calonge, F J [CIEMAT, Madrid (Spain); Cabrillo, I; Caballero, I G; Marco, R; Matorras, F [IFCA, Santander (Spain); Flix, J; Merino, G [PIC, Barcelona (Spain)], E-mail: jose.hernandez@ciemat.es

2008-07-15

An overview of the data transfer, processing and analysis operations conducted at the Spanish Tier-1 (PIC, Barcelona) and Tier-2 (CIEMAT-Madrid and IFCA-Santander federation) centres during the past CMS CSA06 Computing, Software and Analysis challenge and in preparation for CSA07 is present0008.

Canine parvovirus type 2 vaccine protects against virulent challenge with type 2c virus.

Science.gov (United States)

Spibey, N; Greenwood, N M; Sutton, D; Chalmers, W S K; Tarpey, I

2008-04-01

The ability of dogs vaccinated with a live attenuated CPV type 2 (Nobivac Intervet) vaccine to resist challenge with a current CPV2c isolate was investigated. Six SPF beagle dogs were given the minimum recommended course of vaccination, comprising a single inoculation of vaccine (Nobivac Lepto+Nobivac Pi) at 8-10 weeks of age followed 3 weeks later with a parvovirus vaccine in combination with distemper, adenovirus and parainfluenza virus (Nobivac DHPPi) and a repeat leptospirosis vaccine. Six control dogs were kept unvaccinated. All animals were challenged orally with a type 2c isolate of CPV and monitored for clinical signs, virus shedding, white blood cell fluctuations and serological responses. All vaccinated dogs were fully protected; showing no clinical signs nor shedding challenge virus in the faeces, in contrast to control animals, which displayed all the typical signs of infection with pathogenic CPV and shed challenge virus in the faeces.

Challenges in the Setup of Large-scale Next-Generation Sequencing Analysis Workflows

Directory of Open Access Journals (Sweden)

Pranav Kulkarni

Full Text Available While Next-Generation Sequencing (NGS can now be considered an established analysis technology for research applications across the life sciences, the analysis workflows still require substantial bioinformatics expertise. Typical challenges include the appropriate selection of analytical software tools, the speedup of the overall procedure using HPC parallelization and acceleration technology, the development of automation strategies, data storage solutions and finally the development of methods for full exploitation of the analysis results across multiple experimental conditions. Recently, NGS has begun to expand into clinical environments, where it facilitates diagnostics enabling personalized therapeutic approaches, but is also accompanied by new technological, legal and ethical challenges. There are probably as many overall concepts for the analysis of the data as there are academic research institutions. Among these concepts are, for instance, complex IT architectures developed in-house, ready-to-use technologies installed on-site as well as comprehensive Everything as a Service (XaaS solutions. In this mini-review, we summarize the key points to consider in the setup of the analysis architectures, mostly for scientific rather than diagnostic purposes, and provide an overview of the current state of the art and challenges of the field.

Kinetics of the interactions between yeast elongation factors 1A and 1Balpha, guanine nucleotides, and aminoacyl-tRNA

DEFF Research Database (Denmark)

Gromadski, Kirill B; Schümmer, Tobias; Strømgaard, Anne

2007-01-01

of guanine nucleotides. At the concentrations of nucleotides and factors prevailing in the cell, the overall exchange rate is expected to be in the range of 6 s(-1), which is compatible with the rate of protein synthesis in the cell. eEF1A.GTP binds Phe-tRNA(Phe) with a K(d) of 3 nm, whereas eEF1A.GDP shows...... no significant binding, indicating that eEF1A has similar tRNA binding properties as its prokaryotic homolog, EF-Tu. Udgivelsesdato: 2007-Dec-7...

Analysis of security protocols based on challenge-response

Institute of Scientific and Technical Information of China (English)

LUO JunZhou; YANG Ming

2007-01-01

Security protocol is specified as the procedure of challenge-response, which uses applied cryptography to confirm the existence of other principals and fulfill some data negotiation such as session keys. Most of the existing analysis methods,which either adopt theorem proving techniques such as state exploration or logic reasoning techniques such as authentication logic, face the conflicts between analysis power and operability. To solve the problem, a new efficient method is proposed that provides SSM semantics-based definition of secrecy and authentication goals and applies authentication logic as fundamental analysis techniques,in which secrecy analysis is split into two parts: Explicit-Information-Leakage and Implicit-Information-Leakage, and correspondence analysis is concluded as the analysis of the existence relationship of Strands and the agreement of Strand parameters. This new method owns both the power of the Strand Space Model and concision of authentication logic.

Vaccination with canine parvovirus type 2 (CPV-2) protects against challenge with virulent CPV-2b and CPV-2c.

Science.gov (United States)

Siedek, Elisabeth M; Schmidt, Holger; Sture, Gordon H; Raue, Rüdiger

2011-01-01

Mutations in canine parvovirus (CPV) field isolates have created concerns regarding the ability of vaccines containing CPV-2 to protect against infection with the newly identified antigenic types CPV-2b and CPV-2c. To address this concern, the efficacy of CPV-2 strain NL-35-D currently in use as a commercial vaccine was demonstrated against an oral challenge with CPV-2b and CPV-2c, respectively. Clinically healthy specific pathogen free Beagle dogs were either vaccinated or treated with water for injection first at 8-9 weeks of age and again at 11-12 weeks of age. All dogs were challenged either with CPV-2b or CPV-2c three weeks after the second vaccination. During the two week period following challenge, clinical signs, white blood cell counts, serology by haemagglutination inhibition (HI) and serum neutralisation tests, and virus shedding by haemagglutination test were assessed. All control dogs developed clinical signs of parvovirosis (including pyrexia and leucopenia) and shed virus. Vaccinated dogs seroconverted (HI titres > or =80), remained healthy throughout the study and shed more than 100 times less virus than controls. In conclusion, vaccination with the low passage, high titre CPV-2 strain NL-35-D cross-protects dogs against virulent challenges with CPV-2b or CPV-2c by preventing disease and substantially reducing viral shedding.

Challenges and Strategies for Hospice Caregivers: A Qualitative Analysis.

Science.gov (United States)

Oliver, Debra Parker; Demiris, George; Washington, Karla T; Clark, Carlyn; Thomas-Jones, Deborah

2017-08-01

Family caregivers of hospice patients are responsible for the day-to-day care of their loved ones during the final months of life. They are faced with numerous challenges. The purpose of this study was to understand the challenges and coping strategies used by hospice caregivers as they care for their family members. This study is a secondary analysis of data from an attention control group in a large randomized controlled trial testing a cognitive behavioral intervention with hospice caregivers. Audiotaped interviews were coded with initial themes and combined into final categories of caregiving challenges and strategies for managing the hospice caregiving experience. Caregivers shared narratives discussing challenges that included their frustrations with patient care issues, emotional challenges, frustrations with various health care systems, financial problems, and personal health concerns. They also discussed coping strategies involving patient care, self-care, and emotional support. The coping techniques were both problem and emotion based. There is a need for interventions that focus on strengthening both problem-based and emotional-based coping skills to improve the caregiving experience. © The Author 2016. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Successful experimental challenge of dogs with canine parvovirus-2.

OpenAIRE

Carman, S; Povey, C

1982-01-01

Withholding food from dogs for 24 hours prior to, and for 48 hours following oral challenge with a gut mucosal homogenate of canine parvovirus-2, was a successful means of reproducing gastroenteric signs of canine parvovirus-2 infection. Twenty-one of 24 dogs, which had previously received various vaccine preparations of mink enteritis virus or were unvaccinated, and which were starved at challenge, developed soft or liquid feces with large or without large clots of mucus. Altered feces were ...

Towards an integral analysis of stereotypes: Challenging the standard guarantee of impartiality

Directory of Open Access Journals (Sweden)

Laura Clérico

2018-05-01

Full Text Available The use of stereotypes to the detriment of the discriminated group should imply questioning the standard guarantee of judicial impartiality. Even taking the analysis of stereotypes seriously, the depth of its consequences has not been sufficiently taken into account in the constitutional and human rights argumentation because it has not challenged with all intensity the standard way in which the guarantee of impartiality is conceived and applied in legal practice and even by the regional court for the protection of human rights. I will defend two central theses: (1 the use of stereotypes necessarily impacts on the analysis of the guarantee of impartiality and (2 impartiality must get rid of the presumption of impartiality.

FISH analysis for diagnostic evaluation of challenging melanocytic lesions.

Science.gov (United States)

Zimmermann, A K; Hirschmann, A; Pfeiffer, D; Paredes, B E; Diebold, J

2010-09-01

The differential diagnosis of malignant melanomas and atypical melanocytic nevi is still a diagnostic challenge. The currently accepted morphologic criteria show substantial interobserver variability, likewise immunohistochemical studies are often not able to discriminate these lesions reliably. Techniques that support diagnostic accuracy are of the greatest importance considering the growing incidence of malignant melanomas and their increase in younger patients. In this study we analyzed the feasibility of fluorescence in situ hybridization (FISH) analysis for the discrimination of malignant and benign melanocytic tumors. A panel of DNA probes was used to detect chromosomal aberrations of chromosomes 6 and 11. On a series of 5 clearly malignant and benign melanocytic tumors we confirmed the applicability of the test. Then we focused on examination of ambiguous melanocytic lesions, where atypical cells are often difficult to relocalize in the 4',6-Diamidino-2-phenylindol (DAPI)-fluorescence stain. FISH analyses were conducted on destained H&E-stained slides. By comparison of the DAPI-image with photos taken from the H&E stain, unambiguous assignment of the FISH results to the conspicuous groups of cells was possible. The results of FISH analysis were consistent with the conventional diagnosis in 11 of 14 small ambiguous lesions. Of the remaining 3 cases, 2 showed FISH-results close to the cut-off level. Comparison of FISH results on thin and thick sections revealed that the cut-off values have to be adapted for 2 microm destained sections. In conclusion, FISH analysis is a useful and applicable tool for assessment of even smallest melanocytic neoplasms, although there will remain unclear cases that cannot be solved even after additional FISH evaluation.

Criterion Validity of the Child's Challenging Behavior Scale, Version 2 (CCBS-2).

Science.gov (United States)

Bourke-Taylor, Helen M; Cordier, Reinie; Pallant, Julie F

The Child's Challenging Behavior Scale, Version 2 (CCBS-2), measures maternal rating of a child's challenging behaviors that compromise maternal mental health. The CCBS-2, the Child Behavior Checklist (CBCL), and the Strengths and Difficulties Questionnaire (SDQ) were compared in a sample of typically developing young Australian children. Criterion validity was investigated by correlating the CCBS-2 with "gold standard" measures (CBCL and SDQ subscales). Data were collected in a cross-sectional survey of mothers (N = 336) of children ages 3-9 yr. Correlations with the CBCL externalizing subscales demonstrated moderate (ρ = .46) to strong (ρ = .66) correlations. Correlations with the SDQ externalizing behaviors subscales were moderate (ρ = .35) to strong (ρ = .60). The criterion validity established in this study strengthens the psychometric properties that support ongoing development of the CCBS-2 as an efficient tool that may identify children in need of further evaluation. Copyright © 2018 by the American Occupational Therapy Association, Inc.

A Content Analysis Concerning the Studies on Challenges Faced by Novice Teachers

Science.gov (United States)

Kozikoglu, Ishak

2017-01-01

The purpose of this research is to analyze the studies concerning challenges faced by novice teachers in terms of various aspects and compare challenges according to location of the studies conducted in Turkey and abroad. A total of 30 research studies were included in detailed analysis. This is a descriptive study based on qualitative research…

Challenges in diabetes mellitus type 2 management in Nepal

DEFF Research Database (Denmark)

Gyawali, Bishal; Ferrario, Alessandra; van Teijlingen, Edwin

2016-01-01

references. Results Diabetes mellitus type 2 is emerging as a major health care problem in Nepal, with rising prevalence and its complications especially in urban populations. Several challenges in diabetes management were identified, including high cost of treatment, limited health care facilities, and lack...... on the prevalence, cost and treatment of diabetes mellitus type 2 and its complications in Nepal and to critically assess the challenges to be addressed to contain the epidemic and its negative economic impact. Design A comprehensive review of available evidence and data sources on prevalence, risk factors, cost...

Transcriptional analysis of disk abalone (Haliotis discus discus) antioxidant enzymes against marine bacteria and virus challenge.

Science.gov (United States)

De Zoysa, Mahanama; Whang, Ilson; Nikapitiya, Chamilani; Oh, Chulhong; Choi, Cheol Young; Lee, Jehee

2011-07-01

Diverse antioxidant enzymes are essential for marine organisms to overcome oxidative stress as well as for the fine-tuning of immune reactions through activating different signal transduction pathways. This study describes the transcriptional analysis of antioxidant enzymes of disk abalone by challenging with bacteria (Vibrio alginolyticus, Vibrio parahemolyticus, and Listeria monocytogenes) and viral hemorrhagic septicemia virus (VHSV). Upon bacteria and VHSV challenge, Manganese superoxide dismutase (MnSOD), Copper, Zinc superoxide dismutase (CuZnSOD), catalase, thioredoxin peroxidase (TPx), Selenium-dependent glutathione peroxidase (SeGPx), and thioredoxin-2 (TRx-2) expression levels were altered in gills, and hemocytes at different magnitudes. In gills, only MnSOD, catalase, and SeGPx genes were completely upregulated by post-challenge of bacterial and VHSV. Among them, SeGPx demonstrated strong upregulation by 16-fold (bacteria) and 2-fold (VHSV) in gills, and 5-fold (bacteria) and 3.0-fold (VHSV) in hemocytes. None of the genes examined were downregulated (in gills and hemocytes) by bacteria challenge even though CuZnSOD and TPx showed downregulation (completely) in hemocytes by VHSV. In general, abalone hemocytes had lower potential to induce antioxidant enzyme transcripts upon bacteria and VHSV challenge than gills. Based upon these results, we suggest that abalones induce oxidative stress in tissues during the bacteria and VHSV challenge, and the identified response of antioxidant enzymes could be supported for maintaining a low-level of reactive oxygen species (ROS) that may serve as a signal for activating immune reactions against pathogenic conditions. Copyright © 2011 Elsevier Ltd. All rights reserved.

Rehabilitation centers: marketing analysis and future challenges.

Science.gov (United States)

Chandra, Ashish; Stroube, William B; Willis, William K

2014-01-01

A rehabilitation center is another form of health care organization that specializes in providing care for particular conditions of patients. Patients admitted in rehab centers range from being accident victims to those suffering with a specific illness. These organizations are becoming extremely valuable in providing patient care services. However, they have not marketed themselves as aggressively as other health care organizations. This article provides an insight regarding rehab centers and examines marketing issues using a SWOT (strengths, weaknesses, opportunities, and threats) analysis. It further provides some future prospects and challenges for marketers of these organizations.

The Mock LISA Data Challenges: from Challenge 1B to Challenge 3

International Nuclear Information System (INIS)

Babak, Stanislav; Porter, Edward K; Gair, Jonathan; Baker, John G; Arnaud, Keith; Benacquista, Matthew J; Cornish, Neil J; Crowder, Jeff; Vallisneri, Michele; Cutler, Curt; Larson, Shane L; Plagnol, Eric; Vecchio, Alberto; Barack, Leor; Blaut, Arkadiusz; Fairhurst, Stephen; Harry, Ian; Gong Xuefei; Khurana, Deepak; Krolak, Andrzej

2008-01-01

The Mock LISA Data Challenges are a programme to demonstrate and encourage the development of LISA data-analysis capabilities, tools and techniques. At the time of this workshop, three rounds of challenges had been completed, and the next was about to start. In this paper we provide a critical analysis of the entries to the latest completed round, Challenge 1B. The entries confirm the consolidation of a range of data-analysis techniques for galactic and massive-black-hole binaries, and they include the first convincing examples of detection and parameter estimation of extreme-mass-ratio inspiral sources. In this paper we also introduce the next round, Challenge 3. Its data sets feature more realistic waveform models (e.g., galactic binaries may now chirp, and massive-black-hole binaries may precess due to spin interactions), as well as new source classes (bursts from cosmic strings, isotropic stochastic backgrounds) and more complicated nonsymmetric instrument noise

ATLAS Data Challenge 2 A massive Monte Carlo production on the GRID

CERN Document Server

González de la Hoz, S; Lozano, J; Salt, J; Fassi, F; March, L; Adams, D; Deng, W; Nevski, P; Smith, J; Yu, D; Zhao, X; Poulard, G; Goossens, L; Nairz, A; Branco, M; Benekos, N C; Sturrock, R; Walker, R; Vetterli, M; Chudoba, J; Tas, P; Duckeck, G; Kennedy, J; Nielsen, J; Wäänänen, A; Bernardet, K; Negri, G; Rebatto, D; De Salvo, A; Perini, L; Vaccarossa, L; Ould-Saada, F; Read, A; Merino, G; Smirnova, O G; Ellert, M; Quing, D; Brochu, F; Gieraltowski, J; Youssef, S; De, K; Oz-turk, N; Sosebee, M; Severini, H; Gardner, R; Mambeli, M; Smirnov, Y; European Grid Conference

2005-01-01

The study and validation of the ATLAS Computing Model started three years ago and will continue for few years in the context of the so-called Data Chal-lenges (DC). DC1 was conducted during 2002-03; the main goals achieved were to set up the simulation data production infrastructure in a real worldwide collaborative effort and to gain experience in exercising an ATLAS wide production model. DC2 (from May until December 2004) is divided into three phases: (i) generate Monte Carlo data using GEANT4 on three different Grid projects: LCG, GRID3 and NorduGrid; (ii) simulate the first pass recon-struction of real data expected in 2007, and (iii) test the Distributed Analysis model. Experience with the use of the system in world-wide DC2 production of ten million events will be presented. We also present how the three Grid fla-vours are operated. Finally we discuss the first prototypes of Distributed Analy-sis systems.

Computational Aspects of Dam Risk Analysis: Findings and Challenges

Directory of Open Access Journals (Sweden)

Ignacio Escuder-Bueno

2016-09-01

Full Text Available In recent years, risk analysis techniques have proved to be a useful tool to inform dam safety management. This paper summarizes the outcomes of three themes related to dam risk analysis discussed in the Benchmark Workshops organized by the International Commission on Large Dams Technical Committee on “Computational Aspects of Analysis and Design of Dams.” In the 2011 Benchmark Workshop, estimation of the probability of failure of a gravity dam for the sliding failure mode was discussed. Next, in 2013, the discussion focused on the computational challenges of the estimation of consequences in dam risk analysis. Finally, in 2015, the probability of sliding and overtopping in an embankment was analyzed. These Benchmark Workshops have allowed a complete review of numerical aspects for dam risk analysis, showing that risk analysis methods are a very useful tool to analyze the risk of dam systems, including downstream consequence assessments and the uncertainty of structural models.

Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum using quantitative real-time RT-PCR.

Directory of Open Access Journals (Sweden)

Jacinta Gimeno

Full Text Available Switchgrass (Panicum virgatum has received a lot of attention as a forage and bioenergy crop during the past few years. Gene expression studies are in progress to improve new traits and develop new cultivars. Quantitative real time PCR (qRT-PCR has emerged as an important technique to study gene expression analysis. For accurate and reliable results, normalization of data with reference genes is essential. In this work, we evaluate the stability of expression of genes to use as reference for qRT-PCR in the grass P. virgatum. Eleven candidate reference genes, including eEF-1α, UBQ6, ACT12, TUB6, eIF-4a, GAPDH, SAMDC, TUA6, CYP5, U2AF, and FTSH4, were validated for qRT-PCR normalization in different plant tissues and under different stress conditions. The expression stability of these genes was verified by the use of two distinct algorithms, geNorm and NormFinder. Differences were observed after comparison of the ranking of the candidate reference genes identified by both programs but eEF-1α, eIF-4a, CYP5 and U2AF are ranked as the most stable genes in the samples sets under study. Both programs discard the use of SAMDC and TUA6 for normalization. Validation of the reference genes proposed by geNorm and NormFinder were performed by normalization of transcript abundance of a group of target genes in different samples. Results show similar expression patterns when the best reference genes selected by both programs were used but differences were detected in the transcript abundance of the target genes. Based on the above research, we recommend the use of different statistical algorithms to identify the best reference genes for expression data normalization. The best genes selected in this study will help to improve the quality of gene expression data in a wide variety of samples in switchgrass.

Eukaryotic elongation factor 1-beta interacts with the 5' untranslated region of the M gene of Nipah virus to promote mRNA translation.

Science.gov (United States)

Uchida, Shotaro; Sato, Hiroki; Yoneda, Misako; Kai, Chieko

2016-09-01

Nipah virus belongs to the genus Henipavirus in the family Paramyxoviridae, and its RNA genome is larger than those of other paramyxoviruses because it has long untranslated regions (UTRs) in each gene. However, the functions of these UTRs are not fully understood. In this study, we investigated the functions of the 5' UTRs and found that the 5' UTR of the M gene upregulated the translation of a reporter gene. Using an RNA pull-down assay, we showed that eukaryotic elongation factor 1-beta (EEF1B2) interacts with nucleotides 81-100 of the M 5' UTR and specifically enhances its translation efficiency. Our results suggest that the M 5' UTR promotes the production of M protein and viral budding by recruiting EEF1B2.

Continuous day-time time series of E-region equatorial electric fields derived from ground magnetic observatory data

Science.gov (United States)

Alken, P.; Chulliat, A.; Maus, S.

2012-12-01

The day-time eastward equatorial electric field (EEF) in the ionospheric E-region plays an important role in equatorial ionospheric dynamics. It is responsible for driving the equatorial electrojet (EEJ) current system, equatorial vertical ion drifts, and the equatorial ionization anomaly (EIA). Due to its importance, there is much interest in accurately measuring and modeling the EEF. However, there are limited sources of direct EEF measurements with full temporal and spatial coverage of the equatorial ionosphere. In this work, we propose a method of estimating a continuous day-time time series of the EEF at any longitude, provided there is a pair of ground magnetic observatories in the region which can accurately track changes in the strength of the EEJ. First, we derive a climatological unit latitudinal current profile from direct overflights of the CHAMP satellite and use delta H measurements from the ground observatory pair to determine the magnitude of the current. The time series of current profiles is then inverted for the EEF by solving the governing electrodynamic equations. While this method has previously been applied and validated in the Peruvian sector, in this work we demonstrate the method using a pair of magnetometers in Africa (Samogossoni, SAM, 0.18 degrees magnetic latitude and Tamanrasset, TAM, 11.5 degrees magnetic latitude) and validate the resulting EEF values against the CINDI ion velocity meter (IVM) instrument on the C/NOFS satellite. We find a very good 80% correlation with C/NOFS IVM measurements and a root-mean-square difference of 9 m/s in vertical drift velocity. This technique can be extended to any pair of ground observatories which can capture the day-time strength of the EEJ. We plan to apply this work to more observatory pairs around the globe and distribute real-time equatorial electric field values to the community.

Trace-level beryllium analysis in the laboratory and in the field: State of the art, challenges, and opportunities

Energy Technology Data Exchange (ETDEWEB)

BRISSON, MICHAEL

2006-03-30

Control of workplace exposure to beryllium is a growing issue in the United States and other nations. As the health risks associated with low-level exposure to beryllium are better understood, the need increases for improved analytical techniques both in the laboratory and in the field. These techniques also require a greater degree of standardization to permit reliable comparison of data obtained from different locations and at different times. Analysis of low-level beryllium samples, in the form of air filters or surface wipes, is frequently required for workplace monitoring or to provide data to support decision-making on implementation of exposure controls. In the United States and the United Kingdom, the current permissible exposure level is 2 {micro}g/m{sup 3} (air), and the United States Department of Energy has implemented an action level of 0.2 {micro}g/m{sup 3} (air) and 0.2 {micro}g/100 cm{sup 2} (surface). These low-level samples present a number of analytical challenges, including (1) a lack of suitable standard reference materials, (2) unknown robustness of sample preparation techniques, (3) interferences during analysis, (4) sensitivity (sufficiently low detection limits), (5) specificity (beryllium speciation), and (6) data comparability among laboratories. Additionally, there is a need for portable, real-time (or near real-time) equipment for beryllium air monitoring and surface wipe analysis that is both laboratory-validated and field-validated in a manner that would be accepted by national and/or international standards organizations. This paper provides a review of the current analytical requirements for trace-level beryllium analysis for worker protection, and also addresses issues that may change those requirements. The current analytical state of the art and relevant challenges facing the analytical community will be presented, followed by suggested criteria for real-time monitoring equipment. Recognizing and addressing these challenges will

Challenges in diabetes mellitus type 2 management in Nepal

DEFF Research Database (Denmark)

Gyawali, Bishal; Ferrario, Alessandra; van Teijlingen, Edwin

2016-01-01

on the prevalence, cost and treatment of diabetes mellitus type 2 and its complications in Nepal and to critically assess the challenges to be addressed to contain the epidemic and its negative economic impact. DESIGN: A comprehensive review of available evidence and data sources on prevalence, risk factors, cost......, complications, treatment, and management of diabetes mellitus type 2 in Nepal was conducted through an online database search for articles published in English between January 2000 and November 2015. Additionally, we performed a manual search of articles and reference lists of published articles for additional...... references. RESULTS: Diabetes mellitus type 2 is emerging as a major health care problem in Nepal, with rising prevalence and its complications especially in urban populations. Several challenges in diabetes management were identified, including high cost of treatment, limited health care facilities...

Economic Analysis on Key Challenges for Sustainable Aquaculture Development

DEFF Research Database (Denmark)

Gedefaw Abate, Tenaw

challenges that could obstruct its sustainable development, such as a lack of suitable feed, which includes fishmeal, fish oil and live feed, and negative environmental externalities. If the aquaculture industry is to reach its full potential, it must be both environmentally and economically sustainable...... environmental externalities. A sustainable supply of high-quality live feeds at reasonable prices is absolutely essential for aquaculture hatcheries because many commercially produced high-value marine fish larval species, such as flounder, grouper, halibut, tuna and turbot, require live feed for their early...... developmental stage. The key challenge in this regard is that the conventional used live feed items, Artemia and rotifers, are nutritionally deficient. Thus, the first main purpose of the thesis is carrying out an economic analysis of the feasibility of commercial production and the use of an alternative live...

Addressing the challenges in using qualitative data in qualitative comparative analysis

NARCIS (Netherlands)

de Block, Debora; Vis, B.

2017-01-01

This paper addresses an issue that so far has received relatively little attention in methodological discussions about Qualitative Comparative Analysis (QCA), namely the challenges researchers face when using qualitative data in QCA analyses. Building on a literature review of 22 empirical studies

Computational Challenges in the Analysis of Petrophysics Using Microtomography and Upscaling

Science.gov (United States)

Liu, J.; Pereira, G.; Freij-Ayoub, R.; Regenauer-Lieb, K.

2014-12-01

Microtomography provides detailed 3D internal structures of rocks in micro- to tens of nano-meter resolution and is quickly turning into a new technology for studying petrophysical properties of materials. An important step is the upscaling of these properties as micron or sub-micron resolution can only be done on the sample-scale of millimeters or even less than a millimeter. We present here a recently developed computational workflow for the analysis of microstructures including the upscaling of material properties. Computations of properties are first performed using conventional material science simulations at micro to nano-scale. The subsequent upscaling of these properties is done by a novel renormalization procedure based on percolation theory. We have tested the workflow using different rock samples, biological and food science materials. We have also applied the technique on high-resolution time-lapse synchrotron CT scans. In this contribution we focus on the computational challenges that arise from the big data problem of analyzing petrophysical properties and its subsequent upscaling. We discuss the following challenges: 1) Characterization of microtomography for extremely large data sets - our current capability. 2) Computational fluid dynamics simulations at pore-scale for permeability estimation - methods, computing cost and accuracy. 3) Solid mechanical computations at pore-scale for estimating elasto-plastic properties - computational stability, cost, and efficiency. 4) Extracting critical exponents from derivative models for scaling laws - models, finite element meshing, and accuracy. Significant progress in each of these challenges is necessary to transform microtomography from the current research problem into a robust computational big data tool for multi-scale scientific and engineering problems.

Analysis of Nordic educational projects designed to meet challenges in society

DEFF Research Database (Denmark)

Liveng, Anne; Manninen, Jyri; Àrnason, Hróbjartur

, process or structure identified in the successful projects: 1.Networking: Planning, implementation and development of the programme is based on networking of various - and even unexpected - organizations and players/participants. 2.Process evaluation: Continuous project development, documentation...... and analysis of results is inte-grated in the project, using Process Evaluation or Action Research 3.Flexibility: Programme offers flexible access and participation, individualized content or individualized study methods (ICT) 4.Focus on needs: A programme focuses on authentic regional, sectorial or individual...... learning providers, trade unions and other agencies supporting human development to create learning inter-ventions to address specific crisis, challenges or changes. In view of recent crises experienced by the Nordic countries The Education and Training Service Centre in Ice-land (www.frae.is) proposed...

DOE Challenge Home Case Study: e2 Homes – Winter Park, Florida

Energy Technology Data Exchange (ETDEWEB)

none,

2013-01-01

This Challenge Home case study describes the first certified DOE Challenge Home as constructed by e2 Homes. Completed in May 2012, the “Wilson Residence” in Winter Park, Florida, is a 4,305-ft2 custom home that scores a HERS 57 without solar and a better than zero net-energy HERS -7 with solar.

MALDI-MS drug analysis in biological samples: opportunities and challenges.

Science.gov (United States)

Steuer, Andrea E; Poetzsch, Michael; Kraemer, Thomas

2016-09-01

Drug analysis represents a large field in different disciplines. Plasma is commonly considered to be the biosample of choice for that purpose. However, concentrations often do not represent the levels present within deeper compartments and therefore cannot sufficiently explain efficacy or toxicology of drugs. MALDI-MS in drug analysis is of great interest for high-throughput quantification and particularly spatially resolved tissue imaging. The current perspective article will deal with challenges and opportunities of MALDI-MS drug analysis in different biological samples. A particular focus will be on hair samples. Recent applications were included, reviewed for their instrumental setup and sample preparation and pros and cons as well as future perspectives are critically discussed.

The Enterprise 2.0 Concept: Challenges on Data and Information Security

Science.gov (United States)

Silva, Ana; Moreira, Fernando; Varajão, João

The Web 2.0 wave has "hit" businesses all over the world, with companies taking advantage of the 2.0 concept and new applications stimulating collaboration between employees, and also with external partners (suppliers, contractors, universities, R&D organizations and others). However, the use of Web 2.0 applications inside organizations has created additional security challenges, especially regarding data and information security. Companies need to be aware of these risks when deploying the 2.0 concept and take a proactive approach on security. In this paper are identified and discussed some of the challenges and risks of the use of Web 2.0 tools, namely when it comes to securing companies' intellectual property.

AMT (3-(2-aminopropyl)indole) and 5-IT (5-(2-aminopropyl)indole): an analytical challenge and implications for forensic analysis.

Science.gov (United States)

Elliott, Simon P; Brandt, Simon D; Freeman, Sally; Archer, Roland P

2013-03-01

5-(2-Aminopropyl)indole (5-IT) and 3-(2-aminopropyl)indole (α-methyltryptamine, AMT) are isomeric substances and their differentiation can be a challenge under routine analytical conditions, especially when reference material is unavailable. 5-IT represents a very recent addition to the battery of new psychoactive substances that are commercially available from online retailers. This report illustrates how subtle differences observed under mass spectral and UV conditions can help to facilitate the differentiation between the two isomers. Analyses included (1)  H and (13) C NMR, GC-EI/CI ion trap MS, applications of several U/HPLC-DAD and HPLC-MS methods. Investigations currently underway also highlight the confirmation that AMT was detected in a number of fatal intoxications. These findings also demonstrate that there is a potential risk of misidentification when dealing with both substances. Copyright © 2012 John Wiley & Sons, Ltd.

Human Factors in Streaming Data Analysis: Challenges and Opportunities for Information Visualization: Human Factors in Streaming Data Analysis

Energy Technology Data Exchange (ETDEWEB)

Dasgupta, Aritra [Pacific Northwest National Laboratory, Richland Washington USA; Arendt, Dustin L. [Pacific Northwest National Laboratory, Richland Washington USA; Franklin, Lyndsey R. [Pacific Northwest National Laboratory, Richland Washington USA; Wong, Pak Chung [Pacific Northwest National Laboratory, Richland Washington USA; Cook, Kristin A. [Pacific Northwest National Laboratory, Richland Washington USA

2017-09-01

Real-world systems change continuously and across domains like traffic monitoring, cyber security, etc., such changes occur within short time scales. This leads to a streaming data problem and produces unique challenges for the human in the loop, as analysts have to ingest and make sense of dynamic patterns in real time. In this paper, our goal is to study how the state-of-the-art in streaming data visualization handles these challenges and reflect on the gaps and opportunities. To this end, we have three contributions: i) problem characterization for identifying domain-specific goals and challenges for handling streaming data, ii) a survey and analysis of the state-of-the-art in streaming data visualization research with a focus on the visualization design space, and iii) reflections on the perceptually motivated design challenges and potential research directions for addressing them.

High intensity focused ultrasound treatment of adenomyosis: The relationship between the features of magnetic resonance imaging on T2 weighted images and the therapeutic efficacy

Energy Technology Data Exchange (ETDEWEB)

Gong, Chunmei [State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science and Technology, Chongqing Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Haifu Hospital, College of Biomedical Engineering, Chongqing Medical University, Chongqing (China); Setzen, Raymond [Department of Obstetrics and Gynecology, Chris Hani Baragwanath Academic Hospital, Johannesburg (South Africa); Liu, Zhongqiong; Liu, Yunchang [State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science and Technology, Chongqing Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Haifu Hospital, College of Biomedical Engineering, Chongqing Medical University, Chongqing (China); Xie, Bin [Department of Ultrasound, Huanggang Central Hospital, Huanggang City, Hubei 438000 (China); Aili, Aixingzi, E-mail: 1819483078@qq.com [Shanghai First Maternity and Infant Health Hospital, Shanghai (China); Zhang, Lian, E-mail: lianwzhang@yahoo.com [State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science and Technology, Chongqing Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Haifu Hospital, College of Biomedical Engineering, Chongqing Medical University, Chongqing (China)

2017-04-15

Objectives: To investigate the relationship between the features of magnetic resonance imaging (MRI) on T2 weighted images (T2WI) and the therapeutic efficacy of high intensity focused ultrasound (HIFU) on adenomyosis. Materials and methods: From January 2011 to November 2015, four hundred and twenty-eight patients with symptomatic adenomyosis were treated with HIFU. Based on the signal intensity and the number of hyperintense foci in the adenomyotic lesions on T2WI, the patients were classified into groups. The day after HIFU ablation patients underwent contrast-enhanced MRI and a comparison was made of non-perfused volume (NPV) ratio, energy efficiency factor (EEF), treatment time, sonication time, and adverse effects. Results: No significant difference in terms of HIFU treatment settings and results was observed between the group of patients with hypointense adenomyotic lesions and the group with isointense adenomyotic lesions (P > 0.05). However, the sonication time and EEF were significantly higher in the group with multiple hyperintense foci compared to the group with few hyperintense foci. The NPV ratio achieved in the lesions with multiple hyperintenese foci was significantly lower than that in the lesions with few hyperintense foci (P < 0.05). No significant difference was observed in the rate of adverse effects between the two groups. Conclusions: Based on our results, the response of the adenomyotic lesions to HIFU treatment is not related to the signal intensity of adenomyotic lesions on T2WI. However, the number of the high signal intensity foci in the adenomyotic lesions on T2WI can be considered as a predictive factor to help select patients for HIFU treatment.

High intensity focused ultrasound treatment of adenomyosis: The relationship between the features of magnetic resonance imaging on T2 weighted images and the therapeutic efficacy

International Nuclear Information System (INIS)

Gong, Chunmei; Setzen, Raymond; Liu, Zhongqiong; Liu, Yunchang; Xie, Bin; Aili, Aixingzi; Zhang, Lian

2017-01-01

Objectives: To investigate the relationship between the features of magnetic resonance imaging (MRI) on T2 weighted images (T2WI) and the therapeutic efficacy of high intensity focused ultrasound (HIFU) on adenomyosis. Materials and methods: From January 2011 to November 2015, four hundred and twenty-eight patients with symptomatic adenomyosis were treated with HIFU. Based on the signal intensity and the number of hyperintense foci in the adenomyotic lesions on T2WI, the patients were classified into groups. The day after HIFU ablation patients underwent contrast-enhanced MRI and a comparison was made of non-perfused volume (NPV) ratio, energy efficiency factor (EEF), treatment time, sonication time, and adverse effects. Results: No significant difference in terms of HIFU treatment settings and results was observed between the group of patients with hypointense adenomyotic lesions and the group with isointense adenomyotic lesions (P > 0.05). However, the sonication time and EEF were significantly higher in the group with multiple hyperintense foci compared to the group with few hyperintense foci. The NPV ratio achieved in the lesions with multiple hyperintenese foci was significantly lower than that in the lesions with few hyperintense foci (P < 0.05). No significant difference was observed in the rate of adverse effects between the two groups. Conclusions: Based on our results, the response of the adenomyotic lesions to HIFU treatment is not related to the signal intensity of adenomyotic lesions on T2WI. However, the number of the high signal intensity foci in the adenomyotic lesions on T2WI can be considered as a predictive factor to help select patients for HIFU treatment.

Early embryonic failure: Expression and imprinted status of candidate genes on human chromosome 21

Energy Technology Data Exchange (ETDEWEB)

Sherman, L.S.; Bennett, P.R.; Moore, G.E. [Queen Charlotte`s and Chelsea Hospital, London (United Kingdom)

1994-09-01

Two cases of maternal uniparental (hetero)disomy for human chromosome 21 (mUPD21) have been identified in a systematic search for UPD in 23 cases of early embryonic failure (EEF). Bi-parental origin of the other chromosome pairs was confirmed using specific VNTR probes or dinucleotide repeat analysis. Both maternally and paternally derived isochromosomes 21q have previously been identified in two individuals with normal phenotypes. Full UPD21 has a different mechanism of origin than uniparental isochromosome 21q and its effect on imprinted genes and phenotypic outcome will therefore not necessarily be the same. EEF associated with mUPD21 suggests that developmentally important genes on HSA 21 may be imprinted such that they are only expressed from either the maternally or paternally derived alleles. We have searched for monoallelic expression of candidate genes on HSA 21 in human pregnancy (CBS, IFNAR, COL6A1) using intragenic DNA polymorphisms. These genes were chosen either because their murine homologues lie in imprinted regions or because they are potentially important in embryogenesis. Once imprinted candidate genes have been identified, their methylation status and expression in normal, early embryonic failure and uniparental disomy 21 pregnancies will be studied. At the same time, a larger number of cases of EEF are being examined to further investigate the incidence of UPD21 in this group.

Impact of Hurricane Ivan on the regional longleaf pine growth study: is there a relation to site or stand conditions?

Science.gov (United States)

John S. Kush; John C. Gilbert

2010-01-01

The US Forest Service Regional Longleaf Pine Growth Study (RLGS) began its eighth re-measurement (40th year) during 2004 autumn. The study has 305 plots of which 171 plots are located on the Escambia Experimental Forest (EEF) in Brewton AL. EEF is operated by the U.S. Forest Service in cooperation with the T.R. Miller Mill Company. The RLGS has plots distributed across...

Longleaf pine regeneration following Hurricane Ivan utilizing the RLGS plots

Science.gov (United States)

John C. Gilbert; John S. Kush

2013-01-01

On September 16, 2004, Hurricane Ivan hit the Alabama coast and severely impacted numerous plots in the U.S. Forest Service’s Regional Longleaf Growth Study (RLGS). The Escambia Experimental Forest (EEF) has 201 of the 325 RLGS plots. Nearly one-third of the EEF was impacted. Nine plots with pole-sized trees were entirely lost. Another 54 plots had some type of damage...

Coeur d'Alene Tribe Energy Efficiency Feasibility Study

Energy Technology Data Exchange (ETDEWEB)

Allgood, Tiffany L. [Coeur d' Alene Tribe, Plummer, ID (United States); Sorter, Andy [Coeur d' Alene Tribe, Plummer, ID (United States)

2014-09-01

The Coeur d'Alene Tribe's Energy Efficiency Feasibility Study (EEFS) is the culminating document that compiles the energy efficiency and building performance assessment and project prioritization process completed on 36 Tribally owned and operated facilities within Tribal lands. The EEFS contains sections on initial findings, utility billing analyses, energy conservation measures and prioritization and funding sources and strategies for energy project implementation.

Occupational exposure to HDI: progress and challenges in biomarker analysis.

Science.gov (United States)

Flack, Sheila L; Ball, Louise M; Nylander-French, Leena A

2010-10-01

1,6-Hexamethylene diisocyanate (HDI) is extensively used in the automotive repair industry and is a commonly reported cause of occupational asthma in industrialized populations. However, the exact pathological mechanism remains uncertain. Characterization and quantification of biomarkers resulting from HDI exposure can fill important knowledge gaps between exposure, susceptibility, and the rise of immunological reactions and sensitization leading to asthma. Here, we discuss existing challenges in HDI biomarker analysis including the quantification of N-acetyl-1,6-hexamethylene diamine (monoacetyl-HDA) and N,N'-diacetyl-1,6-hexamethylene diamine (diacetyl-HDA) in urine samples based on previously established methods for HDA analysis. In addition, we describe the optimization of reaction conditions for the synthesis of monoacetyl-HDA and diacetyl-HDA, and utilize these standards for the quantification of these metabolites in the urine of three occupationally exposed workers. Diacetyl-HDA was present in untreated urine at 0.015-0.060 μg/l. Using base hydrolysis, the concentration range of monoacetyl-HDA in urine was 0.19-2.2 μg/l, 60-fold higher than in the untreated samples on average. HDA was detected only in one sample after base hydrolysis (0.026 μg/l). In contrast, acid hydrolysis yielded HDA concentrations ranging from 0.36 to 10.1 μg/l in these three samples. These findings demonstrate HDI metabolism via N-acetylation metabolic pathway and protein adduct formation resulting from occupational exposure to HDI. Copyright © 2010 Elsevier B.V. All rights reserved.

Examination of an antecedent communication intervention to reduce tangibly maintained challenging behavior: A controlled analog analysis

NARCIS (Netherlands)

O'Reilly, M.F.; Fragale, C.; Gainey, S.; Kang, S.Y.; Koch, H.; Shubert, J.; El Zein, F.; Longino, D.; Chung, M.; Xu, Z.W.; White, P.J.; Lang, R.B.; Davis, T.; Rispoli, M.; Lancioni, G.E.; Didden, H.C.M.; Healy, O.; Kagohara, D.; Meer, L. van der; Sigafoos, J.

2012-01-01

We examined the influence of an antecedent communication intervention on challenging behavior for three students with developmental disorders. Students were taught to request tangible items that were identified as reinforcers for challenging behavior in a prior functional analysis. individual

Development of probabilistic seismic hazard analysis for international sites, challenges and guidelines

Energy Technology Data Exchange (ETDEWEB)

Fernandez Ares, Antonio, E-mail: antonio.fernandez@rizzoassoc.com [Paul C. Rizzo Associates, Inc., 500 Penn Center Boulevard, Penn Center East, Suite 100, Pittsburgh, PA 15235 (United States); Fatehi, Ali, E-mail: ali.fatehi@rizzoassoc.com [Paul C. Rizzo Associates, Inc., 500 Penn Center Boulevard, Penn Center East, Suite 100, Pittsburgh, PA 15235 (United States)

2013-06-15

Research highlights: ► Site-specific seismic hazard study and suggestions for overcoming those challenges that are inherent to the significant amounts of epistemic uncertainty for sites at remote locations. ► Main aspects of probabilistic seismic hazard analysis (PSHA). ► Regional and site geology in the context of a probabilistic seismic hazard analysis (PSHA), including state-of-the-art ground motion estimation methods, and geophysical conditions. ► Senior seismic hazard analysis (SSHAC) as a mean to incorporate the opinions and contributions of the informed scientific community. -- Abstract: This article provides guidance to conduct a site-specific seismic hazard study, giving suggestions for overcoming those challenges that are inherent to the significant amounts of epistemic uncertainty for sites at remote locations. The text follows the general process of a seismic hazard study, describing both the deterministic and probabilistic approaches. Key and controversial items are identified in the areas of recorded seismicity, seismic sources, magnitude, ground motion models, and local site effects. A case history corresponding to a seismic hazard study in the Middle East for a Greenfield site in a remote location is incorporated along the development of the recommendations. Other examples of analysis case histories throughout the World are presented as well.

Substance misuse prevention and economic analysis: challenges and opportunities regarding international utility.

Science.gov (United States)

Guyll, Max; Spoth, Richard; Cornish, Marilyn A

2012-01-01

Economic analyses of substance misuse prevention assess the intervention cost necessary to achieve a particular outcome, and thereby provide an additional dimension for evaluating prevention programming. This article reviews several types of economic analysis, considers how they can be applied to substance misuse prevention, and discusses challenges to enhancing their international relevance, particularly their usefulness for informing policy decisions. Important first steps taken to address these challenges are presented, including the disease burden concept and the development of generalized cost-effectiveness, advances that facilitate international policy discussions by providing a common framework for evaluating health care needs and program effects.

Regulation of oxidative enzyme activity and eukaryotic elongation factor 2 in human skeletal muscle: influence of gender and exercise

DEFF Research Database (Denmark)

Roepstorff, Carsten; Schjerling, P.; Vistisen, Bodil

2005-01-01

AIM: To investigate gender-related differences in the responses of oxidative enzymes and eukaryotic elongation factor-2 (eEF2) to exercise. METHODS: The influence of exercise (90 min, 60%VO(2peak)) on citrate synthase (CS) and beta-hydroxyacyl-CoA dehydrogenase (HAD) activity and mRNA content...... expression and phosphorylation were unaffected by training status (NS). CONCLUSION: Basal transcriptional, translational, and/or post-translational control of CS and HAD seems to be gender-dependent. Also, gender differences in translation and/or post-translational protein modification of CS occur during...... not differ between females and males (NS). In females only, CS activity was enhanced (P differ between UT and ET but, nevertheless, CS activity was 56% higher in ET than in UT volunteers (P

Incorporating "motivation" into the functional analysis of challenging behavior: on the interactive and integrative potential of the motivating operation.

Science.gov (United States)

Langthorne, Paul; McGill, Peter; O'Reilly, Mark

2007-07-01

Sensitivity theory attempts to account for the variability often observed in challenging behavior by recourse to the "aberrant motivation" of people with intellectual and developmental disabilities. In this article, we suggest that a functional analysis based on environmental (challenging environments) and biological (challenging needs) motivating operations provides a more parsimonious and empirically grounded account of challenging behavior than that proposed by sensitivity theory. It is argued that the concept of the motivating operation provides a means of integrating diverse strands of research without the undue inference of mentalistic constructs. An integrated model of challenging behavior is proposed, one that remains compatible with the central tenets of functional analysis.

Chemical Analysis of Plants that Poison Livestock: Successes, Challenges, and Opportunities.

Science.gov (United States)

Welch, Kevin D; Lee, Stephen T; Cook, Daniel; Gardner, Dale R; Pfister, James A

2018-04-04

Poisonous plants have a devastating impact on the livestock industry as well as human health. To fully understand the effects of poisonous plants, multiple scientific disciplines are required. Chemical analysis of plant secondary compounds is key to identifying the responsible toxins, characterizing their metabolism, and understanding their effects on animals and humans. In this review, we highlight some of the successes in studying poisonous plants and mitigating their toxic effects. We also highlight some of the remaining challenges and opportunities with regards to the chemical analysis of poisonous plants.

Rising to the challenges-Reflections on Future-oriented Technology Analysis

OpenAIRE

Georghiou, Luke; Cassingena Harper, Jennifer

2013-01-01

Drawing upon the presentations made at the fourth conference on Future-oriented Technology Analysis, this essay reflects on the implications of the current period of instability and discontinuity for the practice of FTA or foresight. In the past the demand environment for foresight on research and innovation policy favoured application to priority-setting and articulation of demand. New tendencies include a heightened search for breakthrough science and a focus on grand societal challenges. B...

Nurse managers' challenges in project management.

Science.gov (United States)

Suhonen, Marjo; Paasivaara, Leena

2011-11-01

To analyse the challenges that nurse managers meet in project management. Project management done by nurse managers has a significant role in the success of projects conducted in work units. The data were collected by open interviews (n = 14). The participants were nurse managers, nurses and public health nurses. Data analysis was carried out using qualitative content analysis. The three main challenges nurse managers faced in project management in health-care work units were: (1) apathetic organization and management, (2) paralysed work community and (3) cooperation between individuals being discouraged. Nurse managers' challenges in project management can be viewed from the perspective of the following paradoxes: (1) keeping up projects-ensuring patient care, (2) enthusiastic management-effective management of daily work and (3) supporting the work of a multiprofessional team-leadership of individual employees. It is important for nurse managers to learn to relate these paradoxes to one another in a positive way. Further research is needed, focusing on nurse managers' ability to promote workplace spirituality, nurse managers' emotional intelligence and their enthusiasm in small projects. © 2011 Blackwell Publishing Ltd.

Examination of an Antecedent Communication Intervention to Reduce Tangibly Maintained Challenging Behavior: A Controlled Analog Analysis

Science.gov (United States)

O'Reilly, Mark; Fragale, Christina; Gainey, Summer; Kang, Soyeon; Koch, Heather; Shubert, Jennifer; El Zein, Farah; Longino, Deanna; Chung, Moon; Xu, Ziwei; White, Pamela; Lang, Russell; Davis, Tonya; Rispoli, Mandy; Lancioni, Giulio; Didden, Robert; Healy, Olive; Kagohara, Deborah; van der Meer, Larah; Sigafoos, Jeff

2012-01-01

We examined the influence of an antecedent communication intervention on challenging behavior for three students with developmental disorders. Students were taught to request tangible items that were identified as reinforcers for challenging behavior in a prior functional analysis. Individual participant multielement and reversal designs were used…

Vindicating virtue: a critical analysis of the situationist challenge against Aristotelian moral psychology.

Science.gov (United States)

Croom, Adam M

2014-03-01

This article provides a critical analysis of the situationist challenge against Aristotelian moral psychology. It first outlines the details and results from four paradigmatic studies in psychology that situationists have heavily drawn upon in their critique of the Aristotelian conception of virtuous characteristics, including studies conducted by Hartshorne and May (1928), Darley and Batson (Journal of Personality and Social Psychology 27:100-108, 1973), Isen and Levin (Journal of Personality and Social Psychology 21:384-388, 1972), and Milgram (Journal of Abnormal and Social Psychology 67:371-378, 1963). It then presents ten problems with the way situationists have used these studies to challenge Aristotelian moral psychology. After challenging the situationists on these grounds, the article then proceeds to challenge the situationist presentation of the Aristotelian conception, showing that situationists have provided an oversimplified caricature of it that goes against the grain of much Aristotelian text. In evaluating the situationist challenge against the actual results from empirical research as well as primary Aristotelian text, it will be shown that the situationist debate has advanced both an extreme, untenable view about the nature of characteristics and situations, as well as an inaccurate presentation of the Aristotelian view.

Addressing challenges to MMPI-2-RF-based testimony: questions and answers.

Science.gov (United States)

Ben-Porath, Yossef S

2012-11-01

Introduction of a new version of a psychological test brings with it challenges that can be accentuated by the adversarial nature of the legal process. In the case of the Minnesota Multiphasic Personality Inventory-2 Restructured Form (MMPI-2-RF), these challenges can be addressed by becoming familiar with the rationale for and the methods used in revising the inventory, the information contained in the test manuals, and the growing peer-reviewed literature on the test. Potential challenges to MMPI-2-RF-based testimony are identified in this article and discussed in question and answer format. The questions guiding this discussion are based on the Daubert factors, established in 1993 by the US Supreme Court as criteria for gauging the scientific validity of proffered expert testimony. The answers to these questions apply more broadly to testimony in depositions, pre-trial hearings, and at trial. Consideration of the MMPI-2-RF in light of the Daubert factors indicates that the instrument has been subjected to extensive empirical testing and that a substantial peer-reviewed literature is available to guide and support its use. Information about the known and potential rate of error associated with MMPI-2-RF scores is available, and standard procedures for administration, scoring, and interpretation of the inventory are detailed in the test administration manual. Indicators of MMPI-2-RF acceptance can be cited, and criticisms of the MMPI-2-RF can be addressed with information available in the test documents and an extensive, modern, and actively growing peer-reviewed literature.

Multifunctional Cellular Materials Based on 2D Nanomaterials: Prospects and Challenges.

Science.gov (United States)

Qiu, Ling; He, Zijun; Li, Dan

2018-01-01

Recent advances in emerging 2D nanomaterial-based cellular materials (2D-CMs) open up new opportunities for the development of next generation cellular solids with exceptional properties. Herein, an overview of the current research status of 2D-CMs is provided and their future opportunities are highlighted. First, the unique features of 2D nanomaterials are introduced to illustrate why these nanoscale building blocks are promising for the development of novel cellular materials and what the new features of 2D nanoscale building blocks can offer when compared to their 0D and 1D counterparts. An in-depth discussion on the structure-property relationships of 2D-CMs is then provided, and the remarkable functions that can be achieved by engineering their cellular architecture are highlighted. Additionally, the use of 2D-CMs to tackle key challenges in different practical applications is demonstrated. In conclusion, a personal perspective on the challenges and future research directions of 2D-CMs is given. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Challenges in GC-MS analysis: Case studies on phenibut and ethylphenidate.

Science.gov (United States)

Lee, Hui Zhi Shirley; Ong, Mei Ching; Lim, Jong Lee Wendy; Yap, Tiong Whei Angeline

2017-08-01

The challenges associated with drug analysis using GC-MS such as thermal degradation, cyclisation or unwanted side reactions causing potential erroneous identification have become evident in view of the high surge in new drugs available in the market. Two case studies illustrated how alternative methods or modifications to existing techniques can help to circumvent the limitations. In the first case study, phenibut which is a GABA analogue, cyclises to 4-phenyl-2-pyrrolidinone under thermal conditions. The identification of phenibut was achieved through derivatisation and identification of its TMS derivative. The second case study, thermal degradation was minimised on drugs of interest methylphenidate and ethylphenidate by reducing the injector port temperature to 200°C and maintaining the GC oven temperature at below 190°C in order to prevent thermal degradation of the drugs of interest. Copyright © 2017 Elsevier B.V. All rights reserved.

A method comparison of photovoice and content analysis: research examining challenges and supports of family caregivers.

Science.gov (United States)

Faucher, Mary Ann; Garner, Shelby L

2015-11-01

The purpose of this manuscript is to compare methods and thematic representations of the challenges and supports of family caregivers identified with photovoice methodology contrasted with content analysis, a more traditional qualitative approach. Results from a photovoice study utilizing a participatory action research framework was compared to an analysis of the audio-transcripts from that study utilizing content analysis methodology. Major similarities between the results are identified with some notable differences. Content analysis provides a more in-depth and abstract elucidation of the nature of the challenges and supports of the family caregiver. The comparison provides evidence to support the trustworthiness of photovoice methodology with limitations identified. The enhanced elaboration of theme and categories with content analysis may have some advantages relevant to the utilization of this knowledge by health care professionals. Copyright © 2015 Elsevier Inc. All rights reserved.

ANALYSIS OF UNEMPLOYMENT CHALLENGES IN PALESTINE BETWEEN 2000 AND 2015

Directory of Open Access Journals (Sweden)

Ahmed SALAMA

2017-12-01

Full Text Available This paper discusses the challenges of unemployment in Palestine between 2000 and 2015. The study aims to understand the trends and sources of unemployment in Palestine. Using the Palestinian Central Bureau of Statistics (PCBS data, trend analysis was used to understand the trends of unemployment in both parts of Palestine (West Bank and Gaza Strip between 2000 and 2015. The Results showed that there is a downward trend in the unemployment rate in West Bank and an upward trend appears on the unemployment rate in Gaza Strip. The constraints during the second Intifada and the Siege on Gaza Strip are the main factors that affected on unemployment between 2000 and 2015. The economic growth difference in both parts of Palestine (West Bank and Gaza Strip can be one of the reasons that affecting on unemployment besides the other reasons. Some solutions are introduced for unemployment challenge in Palestine beside the solution of lack of independence issue.

A novel multiple-stage antimalarial agent that inhibits protein synthesis

Science.gov (United States)

Baragaña, Beatriz; Hallyburton, Irene; Lee, Marcus C. S.; Norcross, Neil R.; Grimaldi, Raffaella; Otto, Thomas D.; Proto, William R.; Blagborough, Andrew M.; Meister, Stephan; Wirjanata, Grennady; Ruecker, Andrea; Upton, Leanna M.; Abraham, Tara S.; Almeida, Mariana J.; Pradhan, Anupam; Porzelle, Achim; Martínez, María Santos; Bolscher, Judith M.; Woodland, Andrew; Norval, Suzanne; Zuccotto, Fabio; Thomas, John; Simeons, Frederick; Stojanovski, Laste; Osuna-Cabello, Maria; Brock, Paddy M.; Churcher, Tom S.; Sala, Katarzyna A.; Zakutansky, Sara E.; Jiménez-Díaz, María Belén; Sanz, Laura Maria; Riley, Jennifer; Basak, Rajshekhar; Campbell, Michael; Avery, Vicky M.; Sauerwein, Robert W.; Dechering, Koen J.; Noviyanti, Rintis; Campo, Brice; Frearson, Julie A.; Angulo-Barturen, Iñigo; Ferrer-Bazaga, Santiago; Gamo, Francisco Javier; Wyatt, Paul G.; Leroy, Didier; Siegl, Peter; Delves, Michael J.; Kyle, Dennis E.; Wittlin, Sergio; Marfurt, Jutta; Price, Ric N.; Sinden, Robert E.; Winzeler, Elizabeth A.; Charman, Susan A.; Bebrevska, Lidiya; Gray, David W.; Campbell, Simon; Fairlamb, Alan H.; Willis, Paul A.; Rayner, Julian C.; Fidock, David A.; Read, Kevin D.; Gilbert, Ian H.

2015-06-01

There is an urgent need for new drugs to treat malaria, with broad therapeutic potential and novel modes of action, to widen the scope of treatment and to overcome emerging drug resistance. Here we describe the discovery of DDD107498, a compound with a potent and novel spectrum of antimalarial activity against multiple life-cycle stages of the Plasmodium parasite, with good pharmacokinetic properties and an acceptable safety profile. DDD107498 demonstrates potential to address a variety of clinical needs, including single-dose treatment, transmission blocking and chemoprotection. DDD107498 was developed from a screening programme against blood-stage malaria parasites; its molecular target has been identified as translation elongation factor 2 (eEF2), which is responsible for the GTP-dependent translocation of the ribosome along messenger RNA, and is essential for protein synthesis. This discovery of eEF2 as a viable antimalarial drug target opens up new possibilities for drug discovery.

Group dynamics challenges: Insights from Biosphere 2 experiments

Science.gov (United States)

Nelson, Mark; Gray, Kathelin; Allen, John P.

2015-07-01

Successfully managing group dynamics of small, physically isolated groups is vital for long duration space exploration/habitation and for terrestrial CELSS (Controlled Environmental Life Support System) facilities with human participants. Biosphere 2 had important differences and shares some key commonalities with both Antarctic and space environments. There were a multitude of stress factors during the first two year closure experiment as well as mitigating factors. A helpful tool used at Biosphere 2 was the work of W.R. Bion who identified two competing modalities of behavior in small groups. Task-oriented groups are governed by conscious acceptance of goals, reality-thinking in relation to time and resources, and intelligent management of challenges. The opposing unconscious mode, the "basic-assumption" ("group animal") group, manifests through Dependency/Kill the Leader, Fight/Flight and Pairing. These unconscious dynamics undermine and can defeat the task group's goal. The biospherians experienced some dynamics seen in other isolated teams: factions developing reflecting personal chemistry and disagreements on overall mission procedures. These conflicts were exacerbated by external power struggles which enlisted support of those inside. Nevertheless, the crew evolved a coherent, creative life style to deal with some of the deprivations of isolation. The experience of the first two year closure of Biosphere 2 vividly illustrates both vicissitudes and management of group dynamics. The crew overrode inevitable frictions to creatively manage both operational and research demands and opportunities of the facility, thus staying 'on task' in Bion's group dynamics terminology. The understanding that Biosphere 2 was their life support system may also have helped the mission to succeed. Insights from the Biosphere 2 experience can help space and remote missions cope successfully with the inherent challenges of small, isolated crews.

Group dynamics challenges: Insights from Biosphere 2 experiments.

Science.gov (United States)

Nelson, Mark; Gray, Kathelin; Allen, John P

2015-07-01

Successfully managing group dynamics of small, physically isolated groups is vital for long duration space exploration/habitation and for terrestrial CELSS (Controlled Environmental Life Support System) facilities with human participants. Biosphere 2 had important differences and shares some key commonalities with both Antarctic and space environments. There were a multitude of stress factors during the first two year closure experiment as well as mitigating factors. A helpful tool used at Biosphere 2 was the work of W.R. Bion who identified two competing modalities of behavior in small groups. Task-oriented groups are governed by conscious acceptance of goals, reality-thinking in relation to time and resources, and intelligent management of challenges. The opposing unconscious mode, the "basic-assumption" ("group animal") group, manifests through Dependency/Kill the Leader, Fight/Flight and Pairing. These unconscious dynamics undermine and can defeat the task group's goal. The biospherians experienced some dynamics seen in other isolated teams: factions developing reflecting personal chemistry and disagreements on overall mission procedures. These conflicts were exacerbated by external power struggles which enlisted support of those inside. Nevertheless, the crew evolved a coherent, creative life style to deal with some of the deprivations of isolation. The experience of the first two year closure of Biosphere 2 vividly illustrates both vicissitudes and management of group dynamics. The crew overrode inevitable frictions to creatively manage both operational and research demands and opportunities of the facility, thus staying 'on task' in Bion's group dynamics terminology. The understanding that Biosphere 2 was their life support system may also have helped the mission to succeed. Insights from the Biosphere 2 experience can help space and remote missions cope successfully with the inherent challenges of small, isolated crews. Copyright © 2015 The Committee on

Translation of both 5'TOP and non-TOP host mRNAs continues into the late phase of Baculovirus infection

NARCIS (Netherlands)

Oers, van M.M.; Doitsidou, M.; Thomas, A.A.M.; Maagd, de R.A.; Vlak, J.M.

2003-01-01

Complete cDNA sequences were obtained for ribosomal protein (rp) L15 and eukaryotic initiation factor eIF2 from the lepidopteran insect Spodoptera frugiperda, and for elongation factor eEF2 from S. exigua. The presence of a 5' terminal oligopyrimidine (TOP) tract classified the lepidopteran rpL15

Opportunities and challenges of Web 2.0 within the health care systems: an empirical exploration.

Science.gov (United States)

Lupiáñez-Villanueva, Francisco; Mayer, Miquel Angel; Torrent, Joan

2009-09-01

The Internet has become one of the main drivers of e-health. Whilst its impact and potential is being analysed, the Web 2.0 phenomenon has reached the health field and has emerged as a buzzword that people use to describe a wide range of online activities and applications. The aims of this article are: to explore the opportunities and challenges of the Web 2.0 within the health care system and to identify the gap between the potential of these online activities and applications and the empirical data. The analysis is based on: online surveys to physicians, nurses, pharmacist and patient support groups; static web shot analysis of 1240 web pages and exploration of the most popular Web 2.0 initiatives. The empirical results contrast with the Web 2.0 trends identified. Whereas the main characteristic of the Web 2.0 is the opportunity for social interaction, the health care system at large could currently be characterised by: a lack of interactive communication technologies available on the Internet; a lack of professional production of health care information on the Internet, and a lack of interaction between these professionals and patients on the Internet. These results reveal a scenario away from 2.0 trends.

Effects of oxytocin on high intensity focused ultrasound (HIFU) ablation of adenomysis: A prospective study

International Nuclear Information System (INIS)

Zhang, Xin; Zou, Min; Zhang, Cai; He, Jia; Mao, Shihua; Wu, Qingrong; He, Min; Wang, Jian; Zhang, Ruitao; Zhang, Lian

2014-01-01

Objective: To investigate the effects of oxytocin on high-intensity focused ultrasound (HIFU) ablation for the treatment of adenomyosis. Materials and methods: Eighty-six patients with adenomyosis from three hospitals were randomly assigned to the oxytocin group or control group for HIFU treatment. During HIFU treatment, 80 units of oxytocin was added in 500 ml of 0.9% normal saline running at the rate of 2 ml/min (0.32 U/min) in the oxytocin group, while 0.9% normal saline was used in the control group. Both patients and HIFU operators were blinded to oxytocin or saline application. Treatment results, adverse effects were compared. Results: When using oxytocin, the non-perfused volume (NPV) ratio was 80.7 ± 11.6%, the energy-efficiency factor (EEF) was 8.1 ± 9.9 J/mm 3 , and the sonication time required to ablate 1 cm 3 was 30.0 ± 36.0 s/cm 3 . When not using oxytocin, the non-perfused volume ratio was 70.8 ± 16.7%, the EEF was 15.8 ± 19.6 J/mm 3 , and the sonication time required to ablate 1 cm 3 was 58.2 ± 72.7 S/cm 3 . Significant difference in the NPV ratio, EEF, and the sonication time required to ablate 1 cm 3 between the two groups was observed. No oxytocin related adverse effects occurred. Conclusion: Oxytocin could significantly decrease the energy for ablating adenomyosis with HIFU, safely enhance the treatment efficiency

Understanding Challenges, Strategies, and the Role of Support Networks in Medication Self-management Among Patients With Type 2 Diabetes.

Science.gov (United States)

Bernhard, Gerda; Ose, Dominik; Baudendistel, Ines; Seidling, Hanna M; Stützle, Marion; Szecsenyi, Joachim; Wensing, Michel; Mahler, Cornelia

2017-04-01

Purpose The purpose of this qualitative study was to investigate the challenges and strategies of patients with type 2 diabetes mellitus (T2DM) regarding daily management of their medication regimen focusing on the role of their support networks. Methods A purposeful sample of 25 patients with T2DM was recruited from local self-help groups, general practitioner practices, and a university hospital in southwestern Germany. Four semi-structured focus groups were conducted to identify the challenges patients experienced, the strategies they used, and their collaboration with support networks to assist them in self-managing their medication regimen. Sessions were audio- and video-recorded, fully transcribed, and subjected to computer-aided qualitative content analysis, guided by the Self- and Family Management Framework (SFMF). Results Patients with T2DM experienced numerous challenges affecting medication self-management arising from their personal situation, health status and resources, characteristics of their regimen, and how health care is currently organized. Patients' self-initiated strategies included activating health care, community, social, and online resources; taking ownership of medication-related needs; and integrating medication-taking into daily life. Patients drew on self-help groups, family, and friends to discuss concerns regarding medication safety and receive experience-based information and advice for navigating within the health care system as well as practical hands-on support with daily medication self-management. Conclusions Understanding the challenges and building on strategies patients with T2DM devised help diabetes educators to better address patients' needs and priorities and guide patient-centered interventions to support patients' self-management activities. Community and social support networks operating in patients' lives need to be engaged in the self-management support.

Prospects and challenges for the recovery of 2-butanol produced by vacuum fermentation - a techno-economic analysis.

Science.gov (United States)

Pereira, Joana P C; Lopez-Gomez, Gustavo; Reyes, Noelia G; van der Wielen, Luuk A M; Straathof, Adrie J J

2017-07-01

The conceptual design of a bio-based process for 2-butanol production is presented for the first time. Considering a hypothetical efficient producing strain, a vacuum fermentation is proposed to alleviate product toxicity, but the main challenge is the energy-efficient product recovery from the vapor. Three downstream scenarios were examined for this purpose: 1) multi-stage vapor recompression; 2) temperature swing adsorption; and 3) vapor absorption. The processes were simulated using Aspen Plus, considering a production capacity of 101 kton/yr. Process optimization was performed targeting the minimum selling price of 2-butanol. The feasibility of the different configurations was analyzed based on the global energy requirements and capital expenditure. The use of integrated adsorption and absorption minimized the energy duty required for azeotrope purification, which represents 11% of the total operational expenditure in Scenario 1. The minimum selling price of 2-butanol as commodity chemical was estimated as 1.05 $/kg, 1.21 $/kg, and 1.03 $/kg regarding the fermentation integrated with downstream scenarios 1), 2), and 3), respectively. Significant savings in 2-butanol production could be achieved in the suggested integrated configurations if more efficient microbial strains were engineered, and more selective adsorption and absorption materials were found for product recovery. Copyright © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

The Mock LISA Data Challenges: from challenge 3 to challenge 4

International Nuclear Information System (INIS)

Babak, Stanislav; Petiteau, Antoine; Robinson, Emma L; Baker, John G; McWilliams, Sean T; Arnaud, Keith A; Benacquista, Matthew J; Cornish, Neil J; Adams, Matt; Larson, Shane L; Mandel, Ilya; Porter, Edward K; Vallisneri, Michele; Cutler, Curt; Vecchio, Alberto; Blaut, Arkadiusz; Bridges, Michael; Feroz, Farhan; Cohen, Michael; Gair, Jonathan R.

2010-01-01

The Mock LISA Data Challenges are a program to demonstrate LISA data-analysis capabilities and to encourage their development. Each round of challenges consists of one or more datasets containing simulated instrument noise and gravitational waves from sources of undisclosed parameters. Participants analyze the datasets and report best-fit solutions for the source parameters. Here we present the results of the third challenge, issued in April 2008, which demonstrated the positive recovery of signals from chirping galactic binaries, from spinning supermassive-black-hole binaries (with optimal SNRs between ∼10 and 2000), from simultaneous extreme-mass-ratio inspirals (SNRs of 10-50), from cosmic-string-cusp bursts (SNRs of 10-100), and from a relatively loud isotropic background with Ω gw (f) ∼ 10 -11 , slightly below the LISA instrument noise.

The Mock LISA Data Challenges: from challenge 3 to challenge 4

Energy Technology Data Exchange (ETDEWEB)

Babak, Stanislav; Petiteau, Antoine; Robinson, Emma L [Max-Planck-Institut fuer Gravitationsphysik (Albert-Einstein-Institut), Am Muehlenberg 1, D-14476 Golm bei Potsdam (Germany); Baker, John G; McWilliams, Sean T; Arnaud, Keith A [Gravitational Astrophysics Laboratory, NASA Goddard Space Flight Center, 8800 Greenbelt Rd, Greenbelt, MD 20771 (United States); Benacquista, Matthew J [Center for Gravitational Wave Astronomy, University of Texas at Brownsville, Brownsville, TX 78520 (United States); Cornish, Neil J; Adams, Matt [Department of Physics, Montana State University, Bozeman, MT 59717 (United States); Larson, Shane L [Department of Physics, Utah State University, Logan, UT 84322 (United States); Mandel, Ilya [Department of Physics and Astronomy, Northwestern University, Evanston, IL (United States); Porter, Edward K [APC, UMR 7164, University Paris 7 Denis Diderot, 10, rue Alice Domon et Leonie Duquet, 75025 Paris Cedex 13 (France); Vallisneri, Michele; Cutler, Curt [Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109 (United States); Vecchio, Alberto [School of Physics and Astronomy, University of Birmingham, Edgbaston, Birmingham B152TT (United Kingdom); Blaut, Arkadiusz [Institute of Theoretical Physics, University of Wroclaw, Wroclaw (Poland); Bridges, Michael; Feroz, Farhan [Astrophysics Group, Cavendish Laboratory, University of Cambridge, Cambridge CB30HE (United Kingdom); Cohen, Michael [Theoretical Astrophysics, California Institute of Technology, Pasadena, CA 91125 (United States); Gair, Jonathan R., E-mail: Michele.Vallisneri@jpl.nasa.go [Institute of Astronomy, University of Cambridge, Cambridge CB30HA (United Kingdom)

2010-04-21

The Mock LISA Data Challenges are a program to demonstrate LISA data-analysis capabilities and to encourage their development. Each round of challenges consists of one or more datasets containing simulated instrument noise and gravitational waves from sources of undisclosed parameters. Participants analyze the datasets and report best-fit solutions for the source parameters. Here we present the results of the third challenge, issued in April 2008, which demonstrated the positive recovery of signals from chirping galactic binaries, from spinning supermassive-black-hole binaries (with optimal SNRs between approx10 and 2000), from simultaneous extreme-mass-ratio inspirals (SNRs of 10-50), from cosmic-string-cusp bursts (SNRs of 10-100), and from a relatively loud isotropic background with OMEGA{sub gw}(f) approx 10{sup -11}, slightly below the LISA instrument noise.

Identifying and meeting the challenges of insulin therapy in type 2 diabetes

Directory of Open Access Journals (Sweden)

Sorli C

2014-07-01

Full Text Available Christopher Sorli,1,* Michael K Heile2,*1Billings Clinic Research Center, Billings, MT, USA; 2The Family Medical Group Glenway, Cincinnati, OH, USA*Both authors contributed equally to this workAbstract: Type 2 diabetes mellitus (T2DM is a chronic illness that requires clinical recognition and treatment of the dual pathophysiologic entities of altered glycemic control and insulin resistance to reduce the risk of long-term micro- and macrovascular complications. Although insulin is one of the most effective and widely used therapeutic options in the management of diabetes, it is used by less than one-half of patients for whom it is recommended. Clinician-, patient-, and health care system-related challenges present numerous obstacles to insulin use in T2DM. Clinicians must remain informed about new insulin products, emerging technologies, and treatment options that have the potential to improve adherence to insulin therapy while optimizing glycemic control and mitigating the risks of therapy. Patient-related challenges may be overcome by actively listening to the patient's fears and concerns regarding insulin therapy and by educating patients about the importance, rationale, and evolving role of insulin in individualized self-treatment regimens. Enlisting the services of Certified Diabetes Educators and office personnel can help in addressing patient-related challenges. Self-management of diabetes requires improved patient awareness regarding the importance of lifestyle modifications, self-monitoring, and/or continuous glucose monitoring, improved methods of insulin delivery (eg, insulin pens, and the enhanced convenience and safety provided by insulin analogs. Health care system-related challenges may be improved through control of the rising cost of insulin therapy while making it available to patients. To increase the success rate of treatment of T2DM, the 2012 position statement from the American Diabetes Association and the European

The challenges of M2M massive access in wireless cellular networks

Directory of Open Access Journals (Sweden)

Andrea Biral

2015-02-01

Full Text Available The next generation of communication systems, which is commonly referred to as 5G, is expected to support, besides the traditional voice and data services, new communication paradigms, such as Internet of Things (IoT and Machine-to-Machine (M2M services, which involve communication between Machine-Type Devices (MTDs in a fully automated fashion, thus, without or with minimal human intervention. Although the general requirements of 5G systems are progressively taking shape, the technological issues raised by such a vision are still partially unclear. Nonetheless, general consensus has been reached upon some specific challenges, such as the need for 5G wireless access networks to support massive access by MTDs, as a consequence of the proliferation of M2M services. In this paper, we describe the main challenges raised by the M2M vision, focusing in particular on the problems related to the support of massive MTD access in current cellular communication systems. Then we analyze the most common approaches proposed in the literature to enable the coexistence of conventional and M2M services in the current and next generation of cellular wireless systems. We finally conclude by pointing out the research challenges that require further investigation in order to provide full support to the M2M paradigm.

Operational Challenges and Solutions with Implementation of an Adaptive Seamless Phase 2/3 Study

Science.gov (United States)

Spencer, Kimberly; Colvin, Kelly; Braunecker, Brad; Brackman, Marcia; Ripley, Joyce; Hines, Paul; Skrivanek, Zachary; Gaydos, Brenda; Geiger, Mary Jane

2012-01-01

A wide variety of operational issues were encountered with the planning and implementation of an adaptive, dose-finding, seamless phase 2/3 trial for a diabetes therapeutic. Compared with a conventional design, significant upfront planning was required, as well as earlier, more integrated cross-functional coordination. The existing infrastructure necessitated greater flexibility to meet the needs of the adaptive design. Rapid data acquisition, analysis, and reporting were essential to support the successful implementation of the adaptive algorithm. Drug supply for nine treatment arms had to be carefully managed across many sites worldwide. Details regarding these key operational challenges and others will be discussed along with resolutions taken to enable successful implementation of this adaptive, seamless trial. PMID:23294774

Vaccination of dogs with Duramune DAPPi+LC protects against pathogenic canine parvovirus type 2c challenge.

Science.gov (United States)

Wilson, S; Stirling, C; Borowski, S; Thomas, A; King, V; Salt, J

2013-06-22

In this study, we determined whether vaccination with Duramune DAPPi+LC containing canine parvovirus (CPV) type 2b protects against challenge with virulent CPV antigenic type 2c. Seven healthy dogs, seronegative for CPV2, were enrolled into two treatment groups; five were vaccinated twice, 21 days apart, with minimum titre vaccine, and two were given saline. Dogs were challenged with CPV 2c three weeks later. Clinical observations, body weight and rectal temperature measurements, blood samples for serology and white blood cell counts and faecal samples for virus excretion were collected. Control dogs remained seronegative until challenge; vaccinated dogs seroconverted and were positive for antibodies to CPV2 from day 21. Four days after challenge, clinical signs associated with parvovirus infection (vomiting, paroxysmal shivering, depression, loose stools) were observed in the control dogs. Both animals were withdrawn from the study for welfare reasons one day later. On day 47, leucopenia was observed in controls, with white blood cell counts less than 50 per cent prechallenge values. No specific clinical sign of parvovirus infection were observed in the vaccinated dogs, nor was (detectable) challenge virus shed in faeces suggesting that antibodies generated contributed sterilising immunity. We conclude that vaccination of dogs with Duramune DAPPi+LC protects against challenge with a virulent field strain of CPV 2c.

Translation elongation factor 1A facilitates the assembly of the tombusvirus replicase and stimulates minus-strand synthesis.

OpenAIRE

Zhenghe Li; Judit Pogany; Steven Tupman; Anthony M Esposito; Terri Goss Kinzy; Peter D Nagy

2010-01-01

Replication of plus-strand RNA viruses depends on host factors that are recruited into viral replicase complexes. Previous studies showed that eukaryotic translation elongation factor (eEF1A) is one of the resident host proteins in the highly purified tombusvirus replicase complex. Using a random library of eEF1A mutants, we identified one mutant that decreased and three mutants that increased Tomato bushy stunt virus (TBSV) replication in a yeast model host. Additional in vitro assays with w...

Systems analysis of carbon nanotubes: opportunities and challenges for space applications

Science.gov (United States)

Samareh, Jamshid A.; Siochi, Emilie J.

2017-09-01

Recent availability of carbon nanotubes (CNTs) in quantities and formats amenable to producing macroscale components invites consideration of these materials in space applications where their attractive properties can enable the realization of bold concepts for affordable space exploration. The challenge is to identify relevant systems and quantify the benefits at the systems level. Before significant investment or adoption of CNTs for large aerospace systems can be justified, there must be a plausible path to attain the perceived systems level benefits. This challenging step requires a close collaboration among experts on CNTs and aerospace system communities. This paper provides an overview of a few relevant potential CNTs applications for space systems and the gap that must be overcome for deployment of CNTs. It also provides a simple engineering-level systems analysis approach to quantify the benefits of using CNTs over state of the art material solutions.

Training Head Start Teachers to Conduct Trial-Based Functional Analysis of Challenging Behavior

Science.gov (United States)

Rispoli, Mandy; Burke, Mack D.; Hatton, Heather; Ninci, Jennifer; Zaini, Samar; Sanchez, Lisa

2015-01-01

Trial-based functional analysis (TBFA) is a procedure for experimentally identifying the function of challenging behavior within applied settings. The purpose of this study was to examine the effects of a TBFA teacher-training package in the context of two Head Start centers implementing programwide positive behavior support (PWPBS). Four Head…

Addressing the Challenges Related to Transforming Qualitative Into Quantitative Data in Qualitative Comparative Analysis

NARCIS (Netherlands)

Block, de Debora; Vis, Barbara

2018-01-01

The use of qualitative data has so far received relatively little attention in methodological discussions on qualitative comparative analysis (QCA). This article addresses this lacuna by discussing the challenges researchers face when transforming qualitative data into quantitative data in QCA. By

Qualitative analysis of national documents on health care services and pharmaceuticals` purchasing challenges: evidence from Iran.

Science.gov (United States)

Bastani, Peivand; Samadbeik, Mahnaz; Dinarvand, Rassoul; Kashefian-Naeeini, Sara; Vatankhah, Soudabeh

2018-06-05

Iranian health sector encountered many challenges in resource allocation and health service purchasing during the past decades, the aim of this study was to determine the main challenges of the present process of health service purchasing for national policymakers and other developing countries with the same setting. It was a qualitative study carried out via the complete content analysis of all relevant national documents from 2007 to 2014. In order to retrieve the related documents, we searched the official websites related to the Ministry of Health and Medical Education, four main Iranian insurance organizations, the Health Committee of the Parliament Profile, strategic vice president's site and Supreme Insurance Council. After recognition of documents, their credibility and authenticity were evaluated in terms of their publication or adjustment. For the analysis of documents, the four step-Scott method was used applying MAXQDA version 10. Findings illustrated that health service purchase challenges in the country can be classified in 6 main themes of policy-making, executive, intersectional, natural, legal and informational challenges with 26 subthemes. Furthermore, 5 themes of Basic Benefit Package, Reimbursement,Decision making, Technology and Contract are considered as the main Challenges in pharmaceutical purchasing area containing 13 relevant subthemes. It seems that according to documents, Iran has faced many structural and procedural problems with the purchase of the best health interventions. So it is highly recommended to consider consequences derived from the present challenges and try to use these evidences in their policy making process to decrease the existed problems and move to better procurement of health interventions.

Deep sequencing-based transcriptome profiling analysis of bacteria-challenged Lateolabrax japonicus reveals insight into the immune-relevant genes in marine fish

Directory of Open Access Journals (Sweden)

Xiang Li-xin

2010-08-01

Full Text Available Abstract Background Systematic research on fish immunogenetics is indispensable in understanding the origin and evolution of immune systems. This has long been a challenging task because of the limited number of deep sequencing technologies and genome backgrounds of non-model fish available. The newly developed Solexa/Illumina RNA-seq and Digital gene expression (DGE are high-throughput sequencing approaches and are powerful tools for genomic studies at the transcriptome level. This study reports the transcriptome profiling analysis of bacteria-challenged Lateolabrax japonicus using RNA-seq and DGE in an attempt to gain insights into the immunogenetics of marine fish. Results RNA-seq analysis generated 169,950 non-redundant consensus sequences, among which 48,987 functional transcripts with complete or various length encoding regions were identified. More than 52% of these transcripts are possibly involved in approximately 219 known metabolic or signalling pathways, while 2,673 transcripts were associated with immune-relevant genes. In addition, approximately 8% of the transcripts appeared to be fish-specific genes that have never been described before. DGE analysis revealed that the host transcriptome profile of Vibrio harveyi-challenged L. japonicus is considerably altered, as indicated by the significant up- or down-regulation of 1,224 strong infection-responsive transcripts. Results indicated an overall conservation of the components and transcriptome alterations underlying innate and adaptive immunity in fish and other vertebrate models. Analysis suggested the acquisition of numerous fish-specific immune system components during early vertebrate evolution. Conclusion This study provided a global survey of host defence gene activities against bacterial challenge in a non-model marine fish. Results can contribute to the in-depth study of candidate genes in marine fish immunity, and help improve current understanding of host

Ancient human genomes suggest three ancestral populations for present-day Europeans

Science.gov (United States)

Lazaridis, Iosif; Patterson, Nick; Mittnik, Alissa; Renaud, Gabriel; Mallick, Swapan; Kirsanow, Karola; Sudmant, Peter H.; Schraiber, Joshua G.; Castellano, Sergi; Lipson, Mark; Berger, Bonnie; Economou, Christos; Bollongino, Ruth; Fu, Qiaomei; Bos, Kirsten I.; Nordenfelt, Susanne; Li, Heng; de Filippo, Cesare; Prüfer, Kay; Sawyer, Susanna; Posth, Cosimo; Haak, Wolfgang; Hallgren, Fredrik; Fornander, Elin; Rohland, Nadin; Delsate, Dominique; Francken, Michael; Guinet, Jean-Michel; Wahl, Joachim; Ayodo, George; Babiker, Hamza A.; Bailliet, Graciela; Balanovska, Elena; Balanovsky, Oleg; Barrantes, Ramiro; Bedoya, Gabriel; Ben-Ami, Haim; Bene, Judit; Berrada, Fouad; Bravi, Claudio M.; Brisighelli, Francesca; Busby, George B. J.; Cali, Francesco; Churnosov, Mikhail; Cole, David E. C.; Corach, Daniel; Damba, Larissa; van Driem, George; Dryomov, Stanislav; Dugoujon, Jean-Michel; Fedorova, Sardana A.; Romero, Irene Gallego; Gubina, Marina; Hammer, Michael; Henn, Brenna M.; Hervig, Tor; Hodoglugil, Ugur; Jha, Aashish R.; Karachanak-Yankova, Sena; Khusainova, Rita; Khusnutdinova, Elza; Kittles, Rick; Kivisild, Toomas; Klitz, William; Kučinskas, Vaidutis; Kushniarevich, Alena; Laredj, Leila; Litvinov, Sergey; Loukidis, Theologos; Mahley, Robert W.; Melegh, Béla; Metspalu, Ene; Molina, Julio; Mountain, Joanna; Näkkäläjärvi, Klemetti; Nesheva, Desislava; Nyambo, Thomas; Osipova, Ludmila; Parik, Jüri; Platonov, Fedor; Posukh, Olga; Romano, Valentino; Rothhammer, Francisco; Rudan, Igor; Ruizbakiev, Ruslan; Sahakyan, Hovhannes; Sajantila, Antti; Salas, Antonio; Starikovskaya, Elena B.; Tarekegn, Ayele; Toncheva, Draga; Turdikulova, Shahlo; Uktveryte, Ingrida; Utevska, Olga; Vasquez, René; Villena, Mercedes; Voevoda, Mikhail; Winkler, Cheryl; Yepiskoposyan, Levon; Zalloua, Pierre; Zemunik, Tatijana; Cooper, Alan; Capelli, Cristian; Thomas, Mark G.; Ruiz-Linares, Andres; Tishkoff, Sarah A.; Singh, Lalji; Thangaraj, Kumarasamy; Villems, Richard; Comas, David; Sukernik, Rem; Metspalu, Mait; Meyer, Matthias; Eichler, Evan E.; Burger, Joachim; Slatkin, Montgomery; Pääbo, Svante; Kelso, Janet; Reich, David; Krause, Johannes

2014-01-01

We sequenced the genomes of a ~7,000 year old farmer from Germany and eight ~8,000 year old hunter-gatherers from Luxembourg and Sweden. We analyzed these and other ancient genomes1–4 with 2,345 contemporary humans to show that most present Europeans derive from at least three highly differentiated populations: West European Hunter-Gatherers (WHG), who contributed ancestry to all Europeans but not to Near Easterners; Ancient North Eurasians (ANE) related to Upper Paleolithic Siberians3, who contributed to both Europeans and Near Easterners; and Early European Farmers (EEF), who were mainly of Near Eastern origin but also harbored WHG-related ancestry. We model these populations’ deep relationships and show that EEF had ~44% ancestry from a “Basal Eurasian” population that split prior to the diversification of other non-African lineages. PMID:25230663

Engineering program in order to increase the irradiated fuel storage capacity in pool facilities of Juragua

International Nuclear Information System (INIS)

Rodriguez R, J.

1996-01-01

In 1993, a technical program in the spent fuel storage area of Nuclear Plant Juragua was launched. Such a program tries to carry out an engineering assessment of the possibility of increasing the spent fuel storage capacity in pool storage facilities by using high density racks (re-racking) instead of the original (non-compact) ones. The purpose of the above-mentioned program is to evaluate possible solutions that can be applied to the construction works prior to plant operation. The first stage of the program for the 1994-95 period is an ongoing Engineering-Economic Feasibility Study (EEFS), which endeavors to examine the capabilities of the reloading pool in Unit-1 Reactor building and long-term storage pool in auxiliary building in high density storage conditions. Technical details of the EEFS and reached results and difficulties are described. (author). 5 refs., 2 figs

A 5 kA pulsed power supply for inductive and plasma loads in large volume plasma device

Energy Technology Data Exchange (ETDEWEB)

Srivastava, P. K., E-mail: pkumar@ipr.res.in; Singh, S. K.; Sanyasi, A. K.; Awasthi, L. M., E-mail: kushagra.lalit@gmail.com; Mattoo, S. K. [Institute for Plasma Research, Gandhinagar (India)

2016-07-15

This paper describes 5 kA, 12 ms pulsed power supply for inductive load of Electron Energy Filter (EEF) in large volume plasma device. The power supply is based upon the principle of rapid sourcing of energy from the capacitor bank (2.8 F/200 V) by using a static switch, comprising of ten Insulated Gate Bipolar Transistors (IGBTs). A suitable mechanism is developed to ensure equal sharing of current and uniform power distribution during the operation of these IGBTs. Safe commutation of power to the EEF is ensured by the proper optimization of its components and by the introduction of over voltage protection (>6 kV) using an indigenously designed snubber circuit. Various time sequences relevant to different actions of power supply, viz., pulse width control and repetition rate, are realized through optically isolated computer controlled interface.

Interpretative challenges in face analysis

DEFF Research Database (Denmark)

de Oliveira, Sandi Michele; Hernández-Flores, Nieves

2015-01-01

In current research on face analysis questions of who and what should be interpreted, as well as how, are of central interest. In English language research, this question has led to a debate on the concepts of P1 (laypersons, representing the “emic” perspective) and P2 (researchers, representing...... in Spanish and address forms in European Portuguese, we view P1 and P2 as being far more complex than the literature suggests, with subgroups (different types of laypersons and researchers, respectively). At the micro-level we will describe the roles each subgroup plays in the interpretative process...

Identification of a hypothetical membrane protein interactor of ...

Indian Academy of Sciences (India)

Unknown

characterized earlier through co-precipitation studies us- ing antibodies against this conserved carboxyl-terminal region (Rich and Steitz 1987). Protein P0 is also involved at the eEF2 elongation factor-binding domain, as demon- strated in yeast (Justice et al 1999). The P0 protein, and not P1 and P2 proteins, is essential for ...

NASA Centennial Challenge: Three Dimensional (3D) Printed Habitat, Phase 2

Science.gov (United States)

Mueller, Robert P.; Roman, Monserrate C.; Kim, Hong S.

2017-01-01

The NASA Centennial Challenges: 3D-Printed Habitat Challenge seeks to develop the fundamental technologies necessary to manufacture an off-world habitat using mission recycled materials andor local indigenous materials. The vision is that autonomous habitat manufacturing machines will someday be deployed to the Moon or Mars to construct shelters for human habitation.NASA and Bradley University, are holding a new US$ 2.5 million competition to design and build a 3-D printed habitat for deep space exploration, including the agencys journey to Mars.The multi-phase 3-D Printed Habitat Challenge, part of NASA's Centennial Challenges program, is designed to advance the additive construction technology needed to create sustainable housing solutions for Earth and beyond.The first phase of the competition ran through Sept. 27, 2015. This phase, a design competition, called on participants to develop state-of-the-art architectural concepts that take advantage of the unique capabilities 3-D printing offers. The top 3 prizes with a prize purse of $40,000 were awarded at the 2015 World Maker Faire in New York.The second phase of the competition is called the Structural Member Competition and it is divided into three levels happening in the spring and summer of 2017. The Compression Test Competition (Level 1) focuses on the fabrication technologies needed to manufacture structural components from a combination of indigenous materials and recyclables, or indigenous materials alone. For Level 1, teams will develop 3D printable materials, build a 3D printing machine, and print two specimens: a truncated cone and a cylinder. The Level 2 Beam Member Competition is the second of three sub-competitions within the overall Structural Member Competition. For Level 2, teams will print a beam that will be tested.The Level 3 Head to Head Competition is the third of three sub-competitions within the overall Structural Member Competition. For Level 3, teams will develop 3D printable materials

An analysis of challenging behavior, comorbid psychopathology, and Attention-Deficit/Hyperactivity Disorder in Fragile X Syndrome.

LENUS (Irish Health Repository)

Newman, Isabel

2015-03-01

The present study sought to investigate the relationship between challenging behavior, comorbid psychopathology, and Attention-Deficit\\/Hyperactivity Disorder (AD\\/HD) in Fragile X Syndrome (FRAX). Additionally, this study sought to examine how such disorders are predicted by gender, presence of autism spectrum disorder (ASD), and presence of intellectual disability (ID). A total of 47 children and adolescents with FRAX were assessed. Results revealed high levels of challenging behavior and AD\\/HD symptoms within the sample, with some participants exhibiting symptoms of comorbid psychopathology. Further analysis revealed that challenging behavior and comorbid psychopathology were positively correlated, with stereotypy correlating most strongly with comorbid psychopathology. In addition, ASD was found to predict challenging behavior, and gender was found to predict AD\\/HD symptoms. The implications of these findings are discussed.

Morphological and mineral analysis of dental enamel after erosive challenge in gastric juice and orange juice.

Science.gov (United States)

Braga, Sheila Regina Maia; De Faria, Dalva Lúcia Araújo; De Oliveira, Elisabeth; Sobral, Maria Angela Pita

2011-12-01

This study evaluated and compared in vitro the morphology and mineral composition of dental enamel after erosive challenge in gastric juice and orange juice. Human enamel specimens were submitted to erosive challenge using gastric juice (from endoscopy exam) (n = 10), and orange juice (commercially-available) (n = 10), as follows: 5 min in 3 mL of demineralization solution, rinse with distilled water, and store in artificial saliva for 3 h. This cycle was repeated four times a day for 14 days. Calcium (Ca) loss after acid exposure was determined by atomic emission spectroscopy. The presence of carbonate (CO) and phosphate (PO) in the specimens was evaluated before and after the erosive challenge by FT-Raman spectroscopy. Data were tested using t-tests (P enamel was observed in scanning electron microscopy (SEM). The mean loss of Ca was: 12.74 ± 3.33 mg/L Ca (gastric juice) and 7.07 ± 1.44 mg/L Ca (orange juice). The analysis by atomic emission spectroscopy showed statistically significant difference between erosive potential of juices (P = 0.0003). FT-Raman spectroscopy found no statistically significant difference in the ratio CO/PO after the erosive challenge. The CO/PO ratios values before and after the challenge were: 0.16/0.17 (gastric juice) (P = 0.37) and 0.18/0.14 (orange juice) (P = 0.16). Qualitative analysis by SEM showed intense alterations of enamel surface. The gastric juice caused more changes in morphology and mineral composition of dental enamel than orange juice. The atomic emission spectroscopy showed to be more suitable to analyze small mineral loss after erosive challenge than FT-Raman. Copyright © 2011 Wiley Periodicals, Inc.

Using Videoconferencing to Conduct Functional Analysis of Challenging Behavior and Develop Classroom Behavioral Support Plans for Students with Autism

NARCIS (Netherlands)

Machalicek, W.A.; O'Reilly, M.F.; Chan, J.M.; Lang, R.B.; Rispoli, M.; Davis, T.; Shogren, K.; Sigafoos, J.; Lancioni, G.E.; Antonuzzi, M.; Langthorne, P.; Andrews, A.; Didden, H.C.M.

2009-01-01

We conducted a functional analysis of challenging behavior for two students with autism using widely available videoconferencing equipment (laptop computers equipped with web cameras). Observers used the videoconferencing facilities to collect data on challenging behavior and to instruct the

Analysis of the mass media coverage of the Gates Foundation grand challenges in global health initiative.

Science.gov (United States)

Verma, G

2009-03-01

The Grand Challenges were launched in 2003 by the Gates Foundation and other collaborators to address the health needs of developing countries. This paper outlines the current problem with health research and development in the context of inequality as conveyed by the 90/10 divide. The paper then looks at the focus and nature of press reporting of global health issues by analysing how press articles have portrayed the Grand Challenges in Global Health initiative. Analysis of the mass media illustrates that the focus of reporting on the Grand Challenges tends to be on utilitarian themes, leaving issues related to justice and equity comparatively under-reported.

Type 2 diabetes mellitus in pediatrics: a new challenge.

Science.gov (United States)

Van Name, Michelle; Santoro, Nicola

2013-11-01

The increased prevalence of childhood obesity in the last few years has been accompanied by the increase in prevalence of type 2 diabetes in pediatrics. In this paper, we will review the risk factors and the pathogenic determinants leading to type 2 diabetes in youth. We searched on PubMed with the key words: obesity, type 2 diabetes, children, adolescents, youth, non-alcoholic fatty liver disease, genes and selected those publications written in English that we judged to be relevant to the topic of the review. Based on the data present in the literature, we reviewed the following three topics: 1) the role of ectopic fat deposition, in particular of fatty liver, in the pathogenesis of pediatric type 2 diabetes; 2) the progression to type 2 diabetes in pediatrics and how it differs from adults, and 3) current theraputic options. Type 2 diabetes in youth is a complex disease, creating new challenges in treatment and prevention.

The GREAT3 challenge

International Nuclear Information System (INIS)

Miyatake, H; Mandelbaum, R; Rowe, B

2014-01-01

The GRavitational lEnsing Accuracy Testing 3 (GREAT3) challenge is an image analysis competition that aims to test algorithms to measure weak gravitational lensing from astronomical images. The challenge started in October 2013 and ends 30 April 2014. The challenge focuses on testing the impact on weak lensing measurements of realistically complex galaxy morphologies, realistic point spread function, and combination of multiple different exposures. It includes simulated ground- and space-based data. The details of the challenge are described in [1], and the challenge website and its leader board can be found at http://great3challenge.info and http://great3.projects.phys.ucl.ac.uk/leaderboard/, respectively

Progress in the analysis and interpretation of N2O isotopes: Potential and future challenges

Science.gov (United States)

Mohn, Joachim; Tuzson, Béla; Zellweger, Christoph; Harris, Eliza; Ibraim, Erkan; Yu, Longfei; Emmenegger, Lukas

2017-04-01

In recent years, research on nitrous oxide (N2O) stable isotopes has significantly advanced, addressing an increasing number of research questions in biogeochemical and atmospheric sciences [1]. An important milestone was the development of quantum cascade laser based spectroscopic devices [2], which are inherently specific for structural isomers (15N14N16O vs. 14N15N16O) and capable to collect real-time data with high temporal resolution, complementary to the well-established isotope-ratio mass-spectrometry (IRMS) method. In combination with automated preconcentration, optical isotope ratio spectroscopy (OIRS) has been applied to disentangle source processes in suburban, rural and pristine environments [e.g. 3, 4]. Within the European Metrology Research Programme (EMRP) ENV52 project "Metrology for high-impact greenhouse gases (HIGHGAS)", the quality of N2O stable isotope analysis by OIRS, the comparability between laboratories, and the traceability to the international isotope ratio scales have been addressed. An inter-laboratory comparison between eleven IRMS and OIRS laboratories, organised within HIGHGAS, indicated limited comparability for 15N site preference, i.e. the difference between 15N abundance in central (N*NO) and end (*NNO) position [5]. In addition, the accuracy of the NH4NO3 decomposition reaction, which provides the link between 15N site preference and the international 15N/14N scale, was found to be limited by non-quantitative NH4NO3 decomposition in combination with substantially different isotope enrichment factors for both nitrogen atoms [6]. Results of the HIGHGAS project indicate that the following research tasks have to be completed to foster research on N2O isotopes: 1) develop improved techniques to link the 15N and 18O abundance and the 15N site preference in N2O to the international stable isotope ratio scales; 2) provide N2O reference materials, pure and diluted in an air matrix, to improve inter-laboratory compatibility. These tasks

Child's Challenging Behaviour Scale, Version 2 (CCBS-2): Psychometric Evaluation With Young Children.

Science.gov (United States)

Bourke-Taylor, Helen; Pallant, Julie; Cordier, Reinie

In this article, we evaluate psychometric properties of the Child's Challenging Behaviour Scale, Version 2 (CCBS-2) with mothers of young, typically developing children. A cross-sectional mail survey with Australian mothers (N = 337) included the CCBS-2, the Depression Anxiety Stress Scales, and the Parents' Evaluation of Developmental Status scale. Internal consistency was good, and no gender differences in CCBS-2 scores were significant. Significant results included differences between CCBS-2 scores: among children grouped according to age, among children grouped according to pre- and post-school entry, among mothers grouped according to extent of any symptom type, and between this sample and a previously collected age-matched sample of children with disabilities. Of the properties tested, results support sound psychometrics. The CCBS-2 can be used to differentiate children according to age, school entry, and disability as well as to identify families for potential services in behavior management and mental health. Copyright © 2017 by the American Occupational Therapy Association, Inc.

PEACEKEEPING OPERATIONS MANAGEMENT. AN ANALYSIS OF CHALLENGES, CHANGES IN COMMAND ACTION AND TRAINING NEEDS

Directory of Open Access Journals (Sweden)

Ünsal SIĞRI

2015-10-01

Full Text Available The aim of this paper is to uncover emerging challenges of peacekeeping operations, determine the changes in command actions and its effects on the professional preparation of commanders by analyzing experiences of military officers. To that end the research data were collected by means of structured face-to- face interviews with voluntary participation of fourteen officers, who took charge in various peacekeeping operations. The collected data were analyzed based on the content analysis method. Findings indicate that peacekeeping operations pose specific challenges for peacekeepers, necessitate changes in command action in terms of flexibility and new precautions in terms of preparation of commanders.

Diction and Expression in Error Analysis Can Enhance Academic Writing of L2 University Students

Science.gov (United States)

Sajid, Muhammad

2016-01-01

Without proper linguistic competence in English language, academic writing is one of the most challenging tasks, especially, in various genre specific disciplines by L2 novice writers. This paper examines the role of diction and expression through error analysis in English language of L2 novice writers' academic writing in interdisciplinary texts…

Demographic Analysis from Biometric Data: Achievements, Challenges, and New Frontiers.

Science.gov (United States)

Sun, Yunlian; Zhang, Man; Sun, Zhenan; Tan, Tieniu

2018-02-01

Biometrics is the technique of automatically recognizing individuals based on their biological or behavioral characteristics. Various biometric traits have been introduced and widely investigated, including fingerprint, iris, face, voice, palmprint, gait and so forth. Apart from identity, biometric data may convey various other personal information, covering affect, age, gender, race, accent, handedness, height, weight, etc. Among these, analysis of demographics (age, gender, and race) has received tremendous attention owing to its wide real-world applications, with significant efforts devoted and great progress achieved. This survey first presents biometric demographic analysis from the standpoint of human perception, then provides a comprehensive overview of state-of-the-art advances in automated estimation from both academia and industry. Despite these advances, a number of challenging issues continue to inhibit its full potential. We second discuss these open problems, and finally provide an outlook into the future of this very active field of research by sharing some promising opportunities.

Challenges in the implementation of a quality management system applied to radiometric analysis

Energy Technology Data Exchange (ETDEWEB)

Dias, Danila C.S.; Bonifacio, Rodrigo L.; Nascimento, Marcos R.L.; Silva, Nivaldo C. da; Taddei, Maria Helena T., E-mail: danilacdias@gmail.com [Comissao Nacional de Energia Nuclear (LAPOC/CNEN-MG), Pocos de Caldas, MG (Brazil). Laboratorio de Pocos de Caldas

2015-07-01

The concept of quality in laboratories has been well established as an essential factor in the search for reliable results. Since its first version published (1999), the ISO/IEC 17025 has been applied in the industrial and research fields, in a wide range of laboratorial analyses. However, the implementation of a Quality Management System still poses great challenges to institutions and companies. The purpose of this work is to expose the constraints related to the implementation of ISO/IEC 17025 applied to analytical assays of radionuclides, accomplished by studying the case of the Pocos de Caldas Laboratory of the Brazilian Commission for Nuclear Energy. In this lab, a project of accreditation of techniques involving determination of radionuclides in water, soil, sediment and food samples has been conducted since 2011. The challenges presented by this project arise from the administrative view, where the governmental nature of the institution translates into unlevelled availability resources and the organizational view, whereas QMS requires inevitable changes in the organizational culture. It is important to point out that when it comes to accreditation of analysis involving radioactive elements, many aspects must be treated carefully due to the their very particular nature. Among these concerns are the determination of analysis uncertainties, accessibility to international proficiency studies, international radioactive samples and CRM transportation, the study of parameters on the validation of analytical methods and the lack of documentation and specialized personnel regarding quality at radiometric measurements. Through an effective management system, the institution is overcoming these challenges, moving toward the ISO/IEC 17025 accreditation. (author)

Challenges in the implementation of a quality management system applied to radiometric analysis

International Nuclear Information System (INIS)

Dias, Danila C.S.; Bonifacio, Rodrigo L.; Nascimento, Marcos R.L.; Silva, Nivaldo C. da; Taddei, Maria Helena T.

2015-01-01

The concept of quality in laboratories has been well established as an essential factor in the search for reliable results. Since its first version published (1999), the ISO/IEC 17025 has been applied in the industrial and research fields, in a wide range of laboratorial analyses. However, the implementation of a Quality Management System still poses great challenges to institutions and companies. The purpose of this work is to expose the constraints related to the implementation of ISO/IEC 17025 applied to analytical assays of radionuclides, accomplished by studying the case of the Pocos de Caldas Laboratory of the Brazilian Commission for Nuclear Energy. In this lab, a project of accreditation of techniques involving determination of radionuclides in water, soil, sediment and food samples has been conducted since 2011. The challenges presented by this project arise from the administrative view, where the governmental nature of the institution translates into unlevelled availability resources and the organizational view, whereas QMS requires inevitable changes in the organizational culture. It is important to point out that when it comes to accreditation of analysis involving radioactive elements, many aspects must be treated carefully due to the their very particular nature. Among these concerns are the determination of analysis uncertainties, accessibility to international proficiency studies, international radioactive samples and CRM transportation, the study of parameters on the validation of analytical methods and the lack of documentation and specialized personnel regarding quality at radiometric measurements. Through an effective management system, the institution is overcoming these challenges, moving toward the ISO/IEC 17025 accreditation. (author)

Analysis of Aviation Safety Reporting System Incident Data Associated With the Technical Challenges of the Vehicle Systems Safety Technology Project

Science.gov (United States)

Withrow, Colleen A.; Reveley, Mary S.

2014-01-01

This analysis was conducted to support the Vehicle Systems Safety Technology (VSST) Project of the Aviation Safety Program (AVsP) milestone VSST4.2.1.01, "Identification of VSST-Related Trends." In particular, this is a review of incident data from the NASA Aviation Safety Reporting System (ASRS). The following three VSST-related technical challenges (TCs) were the focus of the incidents searched in the ASRS database: (1) Vechicle health assurance, (2) Effective crew-system interactions and decisions in all conditions; and (3) Aircraft loss of control prevention, mitigation, and recovery.

Coincident-inclusive electrofission angular correlations

International Nuclear Information System (INIS)

Arruda Neto, J.D.T.

1983-08-01

A method for the joint analysis of coincident and inclusive electrofission data, in order to minimize effects of the model dependence of data interpretation, is developed. Explicit calculations of the (e,e'f) angular correlations are presented. The potentialities of the method to the study of sub- and near-barrier properties of the fission process, and to the study of the giant resonances fission mode, are discussed. (Author) [pt

AN OVERVIEW AND ANALYSIS OF ENERGY CHALLENGES IN THE REPUBLIC OF CROATIA

Directory of Open Access Journals (Sweden)

Rada Cristina IRIMIE

2013-06-01

Full Text Available This paper examines the key energy challenges that Croatia faces, how those challenges are likely to evolve in the coming years, and some possible solutions to the issues presented. While there is a wide variety of issues that Croatia will need to address, this paper will address two of the most pressing: reliance on energy imports, and an inadequate power grid and energy infrastructure. After identifying current vulnerabilities that the country is facing, the analysis points out the trends and the ongoing challenges regarding the demand for energy in Croatia. Finally, this paper concludes with the recommendation that Croatia pursue a multi-pronged approach to renewable energy generation, emphasizing decentralized production as the most time and cost effective strategy until the national power grid can be sufficiently upgraded and integrated to support more centralized approaches. Its geographic location, immanent admission to the EU and potential for diverse domestic sources of renewable energy combine to set the stage for a bright future for Croatia’s economic development, as long as the Croatian authorities place a priority on a multi - pronged approach to energy sources on the one hand, while continuing to work to a unified energy infrastructure on the other.

Challenges of Virtual School Leadership

Science.gov (United States)

Richardson, Jayson W.; LaFrance, Jason; Beck, Dennis

2015-01-01

The purpose of this case study was to examine challenges faced by virtual school leaders in the United States. Through semistructured interviews, the researchers explored challenges faced by eighteen leaders of fully online or blended online programs. Analysis revealed six main challenges: funding, staff, accountability, time, parents, and…

Using Videoconferencing to Conduct Functional Analysis of Challenging Behavior and Develop Classroom Behavioral Support Plans for Students with Autism

Science.gov (United States)

Machalicek, Wendy; O'Reilly, Mark; Chan, Jeffrey M.; Lang, Russell; Rispoli, Mandy; Davis, Tonya; Shogren, Karrie; Sigafoos, Jeff; Lancioni, Giulio; Antonucci, Massimo; Langthorne, Paul; Andrews, Alonzo; Didden, Robert

2009-01-01

We conducted a functional analysis of challenging behavior for two students with autism using widely available videoconferencing equipment (laptop computers equipped with web cameras). Observers used the videoconferencing facilities to collect data on challenging behavior and to instruct the therapist conducting the assessment. Results of the…

Modeling and Analysis in Marine Big Data: Advances and Challenges

Directory of Open Access Journals (Sweden)

Dongmei Huang

2015-01-01

Full Text Available It is aware that big data has gathered tremendous attentions from academic research institutes, governments, and enterprises in all aspects of information sciences. With the development of diversity of marine data acquisition techniques, marine data grow exponentially in last decade, which forms marine big data. As an innovation, marine big data is a double-edged sword. On the one hand, there are many potential and highly useful values hidden in the huge volume of marine data, which is widely used in marine-related fields, such as tsunami and red-tide warning, prevention, and forecasting, disaster inversion, and visualization modeling after disasters. There is no doubt that the future competitions in marine sciences and technologies will surely converge into the marine data explorations. On the other hand, marine big data also brings about many new challenges in data management, such as the difficulties in data capture, storage, analysis, and applications, as well as data quality control and data security. To highlight theoretical methodologies and practical applications of marine big data, this paper illustrates a broad view about marine big data and its management, makes a survey on key methods and models, introduces an engineering instance that demonstrates the management architecture, and discusses the existing challenges.

Standardisation of a new model of H9N2/Escherichia coli challenge in broilers in the Lebanon

Directory of Open Access Journals (Sweden)

Elie K. Barbour

2009-06-01

Full Text Available Primary infection by low pathogenic avian influenza (LPAI predisposes for secondary infection by Escherichia coli in poultry, leading to significant economic losses. Future research in control of this ailment requires the establishment of a successful controlled challenge by avian influenza virus (AIV/E. coli. Six groups of broilers (6 birds/group were included for the standardisation of the controlled challenge by AIV/E. coli. Birds in groups 1, 2, 3, 4 and 5 received an intra-tracheal challenge of 0.5 ml of two haemagglutinating units of H9N2 virus at 20 days of age. At the age of 23 days, birds in group 1 received an intra-thoracic (right air sac-E. coli challenge equivalent to 1.6 × 109 colony-forming units (cfu/0.5 ml/bird, while birds in groups 2, 3, 4 and 5 received E. coli by the same route and in the following respective decreasing order of viable cells: 1.6 × 106, 1.6 × 105, 1.6 × 104 and 1.6 × 103 cfu. Birds in control group 6 were deprived of H9N2 and E. coli challenge. Results showed significant early mortality in group 1 that was challenged with the highest number of E. coli, in comparison to groups 2-6 (p0.05. The frequencies of four signs at 2 days and at 5 days post E. coli challenge (conjunctivitis, diarrhoea, ocular exudates and rales in the surviving birds of groups 2-5 were most often higher than those observed in control group 6 (p<0.05. These four signs and five gross lesions (abdominal airsacculitis, left thoracic airsacculitis, pericarditis, right thoracic airsacculitis and tracheitis had a decreasing pattern of frequency related to a decrease in the E. coli count used in the challenge.

Evaluation and Analysis of Eco-Security in Environmentally Sensitive Areas Using an Emergy Ecological Footprint.

Science.gov (United States)

Chen, Han-Shen

2017-01-30

In this paper, the overall ecological and environmental sustainability in the Cing-Jing region in Taiwan is examined. As land use and cover change has been found to be an important analysis method, an emergy ecological footprint model was applied and the eco-security assessed to ensure authorities maintain a balance between ecological preservation and tourism development. While the ecological environment in the Cing-Jing region from 2008 to 2014 was found to be within safe levels, all related indices had increased considerably. A Grey model was used to predict the 2015-2024 ecological carrying capacities, from which it was found that there is expected to be a large increase in per capita ecological footprints (EFs), meaning that in the future there is going to be a larger ecological deficit and a higher ecological pressure index (EFI), with the eco-security predicted to reach a Grade 2 intermediate level in 2022. As the Cing-Jing region is predicted to become ecologically unsustainable, local, regional, and national governments need to implement regulations to strictly control the land use in the Cing-Jing region. This study demonstrated that emergy EF (EEF) theory application can give objective guidance to decision-makers to ensure that recreational non-urban eco-security can be maintained at a safe level.

CDOCKER and lambda λ -dynamics for prospective prediction in D3R Grand Challenge 2

Science.gov (United States)

Ding, Xinqiang; Hayes, Ryan L.; Vilseck, Jonah Z.; Charles, Murchtricia K.; Brooks, Charles L.

2018-01-01

The opportunity to prospectively predict ligand bound poses and free energies of binding to the Farnesoid X Receptor in the D3R Grand Challenge 2 provided a useful exercise to evaluate CHARMM based docking (CDOCKER) and λ-dynamics methodologies for use in "real-world" applications in computer aided drug design. In addition to measuring their current performance, several recent methodological developments have been analyzed retrospectively to highlight best procedural practices in future applications. For pose prediction with CDOCKER, when the protein structure used for rigid receptor docking was close to the crystallographic holo structure, reliable poses were obtained. Benzimidazoles, with a known holo receptor structure, were successfully docked with an average RMSD of 0.97 Å. Other non-benzimidazole ligands displayed less accuracy largely because the receptor structures we chose for docking were too different from the experimental holo structures. However, retrospective analysis has shown that when these ligands were re-docked into their holo structures, the average RMSD dropped to 1.18 Å for all ligands. When sulfonamides and spiros were docked with the apo structure, which agrees more with their holo structure than the structures we chose, five out of six ligands were correctly docked. These docking results emphasize the need for flexible receptor docking approaches. For λ-dynamics techniques, including multisite λ-dynamics (MSλD), reasonable agreement with experiment was observed for the 33 ligands investigated; root mean square errors of 2.08 and 1.67 kcal/mol were obtained for free energy sets 1 and 2, respectively. Retrospectively, soft-core potentials, adaptive landscape flattening, and biasing potential replica exchange (BP-REX) algorithms were critical to model large substituent perturbations with sufficient precision and within restrictive timeframes, such as was required with participation in Grand Challenge 2. These developments, their

Anti-IL-5 attenuates activation and surface density of β2-integrins on circulating eosinophils after segmental antigen challenge

Science.gov (United States)

Johansson, Mats W.; Gunderson, Kristin A.; Kelly, Elizabeth A. B.; Denlinger, Loren C.; Jarjour, Nizar N.; Mosher, Deane F.

2013-01-01

Background IL-5 activates αMβ2 integrin on blood eosinophils in vitro. Eosinophils in bronchoalveolar lavage (BAL) following segmental antigen challenge have activated β2-integrins. Objective To identify roles for IL-5 in regulating human eosinophil integrins in vivo. Methods Blood and BAL eosinophils were analyzed by flow cytometry in ten subjects with allergic asthma who underwent a segmental antigen challenge protocol before and after anti-IL-5 administration. Results Blood eosinophil reactivity with monoclonal antibody (mAb) KIM-127, which recognizes partially activated β2-integrins, was decreased after anti-IL-5. Before anti-IL-5, surface densities of blood eosinophil β2, αM, and αL integrin subunits increased modestly post-challenge. After anti-IL-5, such increases did not occur. Before or after anti-IL-5, surface densities of β2,αM, αL, and αD and reactivity with KIM-127 and mAb CBRM1/5, which recognizes high-activity αMβ2, were similarly high on BAL eosinophils 48 h post-challenge. Density and activation state of β1-integrins on blood and BAL eosinophils were not impacted by anti-IL-5, even though anti-IL-5 ablated a modest post-challenge increase on blood or BAL eosinophils of P-selectin glycoprotein ligand-1 (PSGL-1), a receptor for P-selectin that causes activation of β1-integrins. Forward scatter of blood eosinophils post-challenge was less heterogeneous and on the average decreased after anti-IL-5; however, anti-IL-5 had no effect on the decreased forward scatter of eosinophils in post-challenge BAL compared to eosinophils in blood. Blood eosinophil KIM-127 reactivity at the time of challenge correlated with the percentage of eosinophils in BAL post-challenge. Conclusion and Clinical Relevance IL-5 supports a heterogeneous population of circulating eosinophils with partially activated β2-integrins and is responsible for upregulation of β2-integrins and PSGL-1 on circulating eosinophils following segmental antigen challenge but has

Workplace Challenges: The Impact of Personal Beliefs and the Birth Environment.

Science.gov (United States)

Adams, Ellise D

This article reviews 2 workplace challenges faced by the perinatal nurse: the impact of personal beliefs and issues within the birth environment. It also explores how these challenges inform the birth practices of the perinatal nurse. The methods employed for this review are focus groups and a concept analysis. Two focus groups (n = 14) and a concept analysis based on a process defined by Walker and Avant provided a set of birth practices performed by the perinatal nurse who facilitates normal birth. Assertiveness was identified as a primary attribute of the perinatal nurse and several suggestions are identified as empirical referents or methods of measuring the abstract concepts, to identify the workplace challenges of the perinatal nurse. Development of effective processes, designed to overcome the many challenges facing the perinatal nurse, will assist in improving perinatal care for women and newborns.

Thioredoxin Txnl1/TRP32 Is a Redox-active Cofactor of the 26 S Proteasome

DEFF Research Database (Denmark)

Andersen, Katrine M; Klausen, Louise Kjær; Prag, Søren

2009-01-01

in the cytoplasm and nucleus. Txnl1 has thioredoxin activity with a redox potential of about -250 mV. Mutant Txnl1 with one active site cysteine replaced by serine formed disulfide bonds to eEF1A1, a substrate-recruiting factor of the 26S proteasome. eEF1A1 is therefore a likely physiological substrate....... In response to knock-down of Txnl1, ubiquitin-protein conjugates were moderately stabilised. Hence, Txnl1 is the first example of a direct connection between protein reduction and proteolysis, two major intracellular protein quality control mechanisms....

Reliability of oscillometric central hemodynamic responses to an orthostatic challenge.

Science.gov (United States)

Stoner, Lee; Bonner, Chantel; Credeur, Daniel; Lambrick, Danielle; Faulkner, James; Wadsworth, Daniel; Williams, Michelle A

2015-08-01

Monitoring central hemodynamic responses to an orthostatic challenge may provide important insight into autonomic nervous system function. Oscillometric pulse wave analysis devices have recently emerged, presenting clinically viable options for investigating central hemodynamic properties. The purpose of the current study was to determine whether oscillometric pulse wave analysis can be used to reliably (between-day) assess central blood pressure and central pressure augmentation (augmentation index) responses to a 5 min orthostatic challenge (modified tilt-table). Twenty healthy adults (26.4 y (SD 5.2), 55% F, 24.7 kg/m(2) (SD 3.8)) were tested on 3 different mornings in the fasted state, separated by a maximum of 7 days. Central hemodynamic variables were assessed on the left arm using an oscillometric device. Repeated measures analysis of variance indicated a significant main effect of the modified tilt-table for all central hemodynamic variables (P response to the tilt, central diastolic pressure increased by 4.5 mmHg (CI: 2.6, 6.4), central systolic blood pressure increased by 2.3 (CI: 4.4, 0.16) mmHg, and augmentation index decreased by an absolute - 5.3%, (CI: -2.7, -7.9%). The intra-class correlation coefficient values for central diastolic pressure (0.83-0.86), central systolic blood pressure (0.80-0.87) and AIx (0.79-0.82) were above the 0.75 criterion in both the supine and tilted positions, indicating excellent between-day reliability. Central hemodynamic responses to an orthostatic challenge can be assessed with acceptable between-day reliability using oscillometric pulse wave analysis. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Management of Type 2 Diabetes: A Challenge for Patient and Physician.

Science.gov (United States)

van den Arend, I. J. M.; Stolk, R. P.; Krans, H. M. J.; Grobbee, D. E.; Schrijvers, A. J. P.

2000-01-01

Discusses obstacles involved in the routine clinical practice for type 2 Diabetes Mellitus, a disease associated with serious complications and co-morbidity. States that educating patients to take an active role in their care by changing their diet and habits is a challenge to health care educators. Lists tools patients will need to learn to help…

An Appraisal of Social Network Theory and Analysis as Applied to Public Health: Challenges and Opportunities.

Science.gov (United States)

Valente, Thomas W; Pitts, Stephanie R

2017-03-20

The use of social network theory and analysis methods as applied to public health has expanded greatly in the past decade, yielding a significant academic literature that spans almost every conceivable health issue. This review identifies several important theoretical challenges that confront the field but also provides opportunities for new research. These challenges include (a) measuring network influences, (b) identifying appropriate influence mechanisms, (c) the impact of social media and computerized communications, (d) the role of networks in evaluating public health interventions, and (e) ethics. Next steps for the field are outlined and the need for funding is emphasized. Recently developed network analysis techniques, technological innovations in communication, and changes in theoretical perspectives to include a focus on social and environmental behavioral influences have created opportunities for new theory and ever broader application of social networks to public health topics.

Interfaces and Integration of Medical Image Analysis Frameworks: Challenges and Opportunities.

Science.gov (United States)

Covington, Kelsie; McCreedy, Evan S; Chen, Min; Carass, Aaron; Aucoin, Nicole; Landman, Bennett A

2010-05-25

Clinical research with medical imaging typically involves large-scale data analysis with interdependent software toolsets tied together in a processing workflow. Numerous, complementary platforms are available, but these are not readily compatible in terms of workflows or data formats. Both image scientists and clinical investigators could benefit from using the framework which is a most natural fit to the specific problem at hand, but pragmatic choices often dictate that a compromise platform is used for collaboration. Manual merging of platforms through carefully tuned scripts has been effective, but exceptionally time consuming and is not feasible for large-scale integration efforts. Hence, the benefits of innovation are constrained by platform dependence. Removing this constraint via integration of algorithms from one framework into another is the focus of this work. We propose and demonstrate a light-weight interface system to expose parameters across platforms and provide seamless integration. In this initial effort, we focus on four platforms Medical Image Analysis and Visualization (MIPAV), Java Image Science Toolkit (JIST), command line tools, and 3D Slicer. We explore three case studies: (1) providing a system for MIPAV to expose internal algorithms and utilize these algorithms within JIST, (2) exposing JIST modules through self-documenting command line interface for inclusion in scripting environments, and (3) detecting and using JIST modules in 3D Slicer. We review the challenges and opportunities for light-weight software integration both within development language (e.g., Java in MIPAV and JIST) and across languages (e.g., C/C++ in 3D Slicer and shell in command line tools).

Education export:challenges from the product, place and process perspectives in the case of Finland

OpenAIRE

Zheng, Q. (Qiongfang)

2015-01-01

Abstract This thesis aims to map out the challenges in exporting Finnish education from three dimensions: 1) challenges in maintaining Finnish education quality and reputation; 2) challenges in adapting the Finnish education to another culture context; and 3) challenges in managing the export as an international project business. Qualitative content analysis is used as the research method. The EPA project between Finland an...

Web 2.0: Inherent tensions and evident challenges for education

DEFF Research Database (Denmark)

Dohn, Nina Bonderup

2009-01-01

In upper tertiary educational programmes around the world, the new Web-mediated communication practices termed Web 2.0 are introduced as learning activities with the goal of facilitating learning through collaborative knowledge construction. The aim of this paper is to point to discrepancies...... in the views of learning, knowledge, and the goals of the practice implicit in Web 2.0 and educational practices and to argue that these discrepancies lead to theoretical tensions and practical challenges when Web 2.0 practices are utilized for educational purposes. The article is structured into four main...... parts: First, Web 2.0 is characterized from a practice perspective. Second, some conceptual discrepancies between the "practice logics" of Web 2.0 and educational practices are identified. Third, the question of transcending the discrepancies is raised through a discussion of related pedagogical...

Processing and Analysis of Multichannel Extracellular Neuronal Signals: State-of-the-art and Challenges

Directory of Open Access Journals (Sweden)

Mufti eMahmud

2016-06-01

Full Text Available In recent years multichannel neuronal signal acquisition systems have allowed scientists to focus on research questions which were otherwise impossible. They act as a powerful means to study brain (dysfunctions in in-vivo and in in-vitro animal models. Typically, each session of electrophysiological experiments with multichannel data acquisition systems generate large amount of raw data. For example, a 128 channel signal acquisition system with 16 bits A/D conversion and 20 kHz sampling rate will generate approximately 17 GB data per hour (uncompressed. This poses an important and challenging problem of inferring conclusions from the large amounts of acquired data. Thus, automated signal processing and analysis tools are becoming a key component in neuroscience research, facilitating extraction of relevant information from neuronal recordings in a reasonable time. The purpose of this review is to introduce the reader to the current state-of-the-art of open-source packages for (semiautomated processing and analysis of multichannel extracellular neuronal signals (i.e., neuronal spikes, local field potentials, electroencephalogram, etc., and the existing Neuroinformatics infrastructure for tool and data sharing. The review is concluded by pinpointing some major challenges that are to be faced, which include the development of novel benchmarking techniques, cloud-based distributed processing and analysis tools, as well as defining novel means to share and standardize data.

Genetic variation in GIPR influences the glucose and insulin responses to an oral glucose challenge

DEFF Research Database (Denmark)

Saxena, Richa; Hivert, Marie-France; Langenberg, Claudia

2010-01-01

Glucose levels 2 h after an oral glucose challenge are a clinical measure of glucose tolerance used in the diagnosis of type 2 diabetes. We report a meta-analysis of nine genome-wide association studies (n = 15,234 nondiabetic individuals) and a follow-up of 29 independent loci (n = 6,958-30,620)......Glucose levels 2 h after an oral glucose challenge are a clinical measure of glucose tolerance used in the diagnosis of type 2 diabetes. We report a meta-analysis of nine genome-wide association studies (n = 15,234 nondiabetic individuals) and a follow-up of 29 independent loci (n = 6...

Report on the second Mock LISA data challenge

International Nuclear Information System (INIS)

Babak, Stanislav; Porter, Edward K; Baker, John G; Benacquista, Matthew J; Cornish, Neil J; Littenberg, Tyson B; Crowder, Jeff; Cutler, Curt; Vallisneri, Michele; Larson, Shane L; Vecchio, Alberto; Auger, Gerard; Barack, Leor; Blaut, Arkadiusz; Bloomer, Ed; Clark, James; Brown, Duncan A; Fairhurst, Stephen; Christensen, Nelson; Gair, Jonathan R

2008-01-01

The Mock LISA data challenges are a program to demonstrate LISA data-analysis capabilities and to encourage their development. Each round of challenges consists of several data sets containing simulated instrument noise and gravitational waves from sources of undisclosed parameters. Participants are asked to analyze the data sets and report the maximum information about the source parameters. The challenges are being released in rounds of increasing complexity and realism: here we present the results of Challenge 2, issued in Jan 2007, which successfully demonstrated the recovery of signals from nonspinning supermassive-black-hole binaries with optimal SNRs between ∼10 and 2000, from ∼20 000 overlapping galactic white-dwarf binaries (among a realistically distributed population of 26 million), and from the extreme-mass-ratio inspirals of compact objects into central galactic black holes with optimal SNRs ∼100

Challenges from variation across regions in cost effectiveness analysis in multi-regional clinical trials

Directory of Open Access Journals (Sweden)

Yunbo Chu

2016-10-01

Full Text Available Economic evaluation in the form of cost-effectiveness analysis has become a popular means to inform decisions in healthcare. With multi-regional clinical trials in a global development program becoming a new venue for drug efficacy testing in recent decades, questions in methods for cost-effectiveness analysis in the multi-regional clinical trials setting also emerge. This paper addresses some challenges from variation across regions in cost effectiveness analysis in multi-regional clinical trials. Several discussion points are raised for further attention and a multi-regional clinical trial example is presented to illustrate the implications in industrial application. A general message is delivered to call for a depth discussion by all stakeholders to reach an agreement on a good practice in cost-effectiveness analysis in the multi-regional clinical trials. Meanwhile, we recommend an additional consideration of cost-effectiveness analysis results based on the clinical evidence from a certain homogeneous population as sensitivity or scenario analysis upon data availability.

Statistics Poster Challenge for Schools

Science.gov (United States)

Payne, Brad; Freeman, Jenny; Stillman, Eleanor

2013-01-01

The analysis and interpretation of data are important life skills. A poster challenge for schoolchildren provides an innovative outlet for these skills and demonstrates their relevance to daily life. We discuss our Statistics Poster Challenge and the lessons we have learned.

Research Challenges in Financial Data Modeling and Analysis.

Science.gov (United States)

Alexander, Lewis; Das, Sanjiv R; Ives, Zachary; Jagadish, H V; Monteleoni, Claire

2017-09-01

Significant research challenges must be addressed in the cleaning, transformation, integration, modeling, and analytics of Big Data sources for finance. This article surveys the progress made so far in this direction and obstacles yet to be overcome. These are issues that are of interest to data-driven financial institutions in both corporate finance and consumer finance. These challenges are also of interest to the legal profession as well as to regulators. The discussion is relevant to technology firms that support the growing field of FinTech.

[Personal computer-based computer monitoring system of the anesthesiologist (2-year experience in development and use)].

Science.gov (United States)

Buniatian, A A; Sablin, I N; Flerov, E V; Mierbekov, E M; Broĭtman, O G; Shevchenko, V V; Shitikov, I I

1995-01-01

Creation of computer monitoring systems (CMS) for operating rooms is one of the most important spheres of personal computer employment in anesthesiology. The authors developed a PC RS/AT-based CMS and effectively used it for more than 2 years. This system permits comprehensive monitoring in cardiosurgical operations by real time processing the values of arterial and central venous pressure, pressure in the pulmonary artery, bioelectrical activity of the brain, and two temperature values. Use of this CMS helped appreciably improve patients' safety during surgery. The possibility to assess brain function by computer monitoring the EEF simultaneously with central hemodynamics and body temperature permit the anesthesiologist to objectively assess the depth of anesthesia and to diagnose cerebral hypoxia. Automated anesthesiological chart issued by the CMS after surgery reliably reflects the patient's status and the measures taken by the anesthesiologist.

Low doses of flagellin-L2 multimer vaccines protect against challenge with diverse papillomavirus genotypes.

Science.gov (United States)

Kalnin, Kirill; Tibbitts, Timothy; Yan, Yanhua; Stegalkina, Svetlana; Shen, Lihua; Costa, Victor; Sabharwal, Robert; Anderson, Stephen F; Day, Patricia M; Christensen, Neil; Schiller, John T; Jagu, Subhashini; Roden, Richard B S; Almond, Jeffrey; Kleanthous, Harold

2014-06-12

Genetically modified bacterial flagellin (Fla), a Toll-like receptor-5 (TLR5) ligand, was evaluated as a fusion partner for human papillomavirus (HPV) L2-based immunogens in two animal challenge models; either cutaneous inoculation of rabbits with HPV 'quasivirions' containing cottontail rabbit papillomavirus (CRPV) genomes that induce warts, or intra-vaginal inoculation of mice with HPV 'pseudovirions' encapsidating a luciferase reporter plasmid and measurement of bioluminescence to determine infectivity. An Escherichia coli production system was developed for flagellin-L2 (Fla-L2) fusions containing either monomeric HPV-16 L2 a.a. 11(×11-200) or oligomeric L2 comprising a fusion of the a.a. 11-88 peptides of five (Fla∼5×11-88) or eight (Fla∼8×11-88) genital HPV types. Immunogenicity and bioactivity of Fla-L2 constructs were assessed using an in vitro neutralization and cell-based TLR-5 binding assay, respectively. Efficacy was evaluated following active immunization of rabbits or mice administered 3 intramuscular doses of Fla-L2 recombinants without exogenous adjuvant, followed by challenge. In addition, passive immunization studies of naïve rabbits with serial dilutions of pooled immune sera were used to determine End-Point Protection Titers (EPPT) for each formulation against a broader spectrum of HPV quasivirions. Efficacy was assessed for up to 10 weeks on the basis of wart volume induced following challenge and results compared to licensed L1-VLP vaccines (Gardasil and Cervarix). Following active immunization at doses as low as 1 μg, Fla-L2 fusions afforded complete protection against infection (mice) and disease (rabbits) following either homologous or heterologous HPV challenge. Passive immunization with anti-L2 immune sera discriminated between the different vaccine candidates under evaluation, demonstrated the protective role of antibody and suggested the superiority of this oligomeric L2-TLR5 agonist fusion approach compared to L1-based

Merchant Banks Fortunes And Structureal Challenges Of Universal ...

African Journals Online (AJOL)

Merchant Banks Fortunes And Structureal Challenges Of Universal Banking In The Port Harcourt Business Environment. ... The techniques adopted in the analysis of data included tabulation, simple percentage, and chi-square (X2). The findings indicated that, although the advent of universal banking has opened up a new ...

Performance of the NorduGrid ARC and the Dulcinea Executor in ATLAS Data Challenge 2

DEFF Research Database (Denmark)

Kleist, Josva; Eerola, P; Ekelöf, T.

2004-01-01

This talk describes the various stages of ATLAS Data Challenge 2 (DC2) in what concerns usage of resources deployed via NorduGrid's Advanced Resource Connector (ARC). It also describes the integration of these resources with the ATLAS production system using the Dulcinea executor. ATLAS Data...... Challenge 2 (DC2), run in 2004, was designed to be a step forward in the distributed data processing. In particular, much coordination of task assignment to resources was planned to be delegated to Grid in its different flavours. An automatic production management system was designed, to direct the tasks...... participation in ATLAS DC2. This was the first attempt to harness large amounts of strongly heterogeneous resources in various countries for a single collaborative exercise using Grid tools. This talk addresses various issues that arose during different stages of DC2 in this environment: preparation...

Opportunities and challenges in conducting secondary analysis of HIV programmes using data from routine health information systems and personal health information.

Science.gov (United States)

Gloyd, Stephen; Wagenaar, Bradley H; Woelk, Godfrey B; Kalibala, Samuel

2016-01-01

HIV programme data from routine health information systems (RHIS) and personal health information (PHI) provide ample opportunities for secondary data analysis. However, these data pose unique opportunities and challenges for use in health system monitoring, along with process and impact evaluations. Analyses focused on retrospective case reviews of four of the HIV-related studies published in this JIAS supplement. We identify specific opportunities and challenges with respect to the secondary analysis of RHIS and PHI data. Challenges working with both HIV-related RHIS and PHI included missing, inconsistent and implausible data; rapidly changing indicators; systematic differences in the utilization of services; and patient linkages over time and different data sources. Specific challenges among RHIS data included numerous registries and indicators, inconsistent data entry, gaps in data transmission, duplicate registry of information, numerator-denominator incompatibility and infrequent use of data for decision-making. Challenges specific to PHI included the time burden for busy providers, the culture of lax charting, overflowing archives for paper charts and infrequent chart review. Many of the challenges that undermine effective use of RHIS and PHI data for analyses are related to the processes and context of collecting the data, excessive data requirements, lack of knowledge of the purpose of data and the limited use of data among those generating the data. Recommendations include simplifying data sources, analysis and reporting; conducting systematic data quality audits; enhancing the use of data for decision-making; promoting routine chart review linked with simple patient tracking systems; and encouraging open access to RHIS and PHI data for increased use.

Potential performance analysis and future trend prediction of electric vehicle with V2G/V2H/V2B capability

Directory of Open Access Journals (Sweden)

Dalong Guo

2016-03-01

Full Text Available Due to the intermittent nature, renewable energy sources (RES has brought new challenges on load balancing and energy dispatching to the Smart Grid. Potentially served as distributed energy storage, Electric Vehicle’s (EV battery can be used as a way to help mitigate the pressure of fluctuation brought by RES and reinforce the stability of power systems. This paper gives a comprehensive review of the current situation of EV technology and mainly emphasizing three EV discharging operations which are Vehicle to Grid (V2G, Vehicle to Home (V2H, and Vehicle to Building (V2B, respectively. When needed, EV’s battery can discharge and send its surplus energy back to power grid, residential homes, or buildings. Based on our data analysis, we argue that V2G with the largest transmission power losses is potentially less efficient compared with the other two modes. We show that the residential users have the incentive to schedule the charging, V2G, and V2H according to the real-time price (RTP and the market sell-back price. In addition, we discuss some challenges and potential risks resulting from EVs’ fast growth. Finally we propose some suggestions on future power systems and also argue that some incentives or rewards need to be provided to motivate EV owners to behave in the best interests of the overall power systems.

Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress.

Science.gov (United States)

McLoughlin, Fionn; Basha, Eman; Fowler, Mary E; Kim, Minsoo; Bordowitz, Juliana; Katiyar-Agarwal, Surekha; Vierling, Elizabeth

2016-10-01

The ubiquitous small heat shock proteins (sHSPs) are well documented to act in vitro as molecular chaperones to prevent the irreversible aggregation of heat-sensitive proteins. However, the in vivo activities of sHSPs remain unclear. To investigate the two most abundant classes of plant cytosolic sHSPs (class I [CI] and class II [CII]), RNA interference (RNAi) and overexpression lines were created in Arabidopsis (Arabidopsis thaliana) and shown to have reduced and enhanced tolerance, respectively, to extreme heat stress. Affinity purification of CI and CII sHSPs from heat-stressed seedlings recovered eukaryotic translation elongation factor (eEF) 1B (α-, β-, and γ-subunits) and eukaryotic translation initiation factor 4A (three isoforms), although the association with CI sHSPs was stronger and additional proteins involved in translation were recovered with CI sHSPs. eEF1B subunits became partially insoluble during heat stress and, in the CI and CII RNAi lines, showed reduced recovery to the soluble cell fraction after heat stress, which was also dependent on HSP101. Furthermore, after heat stress, CI sHSPs showed increased retention in the insoluble fraction in the CII RNAi line and vice versa. Immunolocalization revealed that both CI and CII sHSPs were present in cytosolic foci, some of which colocalized with HSP101 and with eEF1Bγ and eEF1Bβ. Thus, CI and CII sHSPs have both unique and overlapping functions and act either directly or indirectly to protect specific translation factors in cytosolic stress granules. © 2016 American Society of Plant Biologists. All Rights Reserved.

Evaluation of suitable reference genes for gene expression studies in bovine muscular tissue

Directory of Open Access Journals (Sweden)

Dunner Susana

2008-09-01

Full Text Available Abstract Background Real-time reverse transcriptase quantitative polymerase chain reaction (real-time RTqPCR is a technique used to measure mRNA species copy number as a way to determine key genes involved in different biological processes. However, the expression level of these key genes may vary among tissues or cells not only as a consequence of differential expression but also due to different factors, including choice of reference genes to normalize the expression levels of the target genes; thus the selection of reference genes is critical for expression studies. For this purpose, ten candidate reference genes were investigated in bovine muscular tissue. Results The value of stability of ten candidate reference genes included in three groups was estimated: the so called 'classical housekeeping' genes (18S, GAPDH and ACTB, a second set of genes used in expression studies conducted on other tissues (B2M, RPII, UBC and HMBS and a third set of novel genes (SF3A1, EEF1A2 and CASC3. Three different statistical algorithms were used to rank the genes by their stability measures as produced by geNorm, NormFinder and Bestkeeper. The three methods tend to agree on the most stably expressed genes and the least in muscular tissue. EEF1A2 and HMBS followed by SF3A1, ACTB, and CASC3 can be considered as stable reference genes, and B2M, RPII, UBC and GAPDH would not be appropriate. Although the rRNA-18S stability measure seems to be within the range of acceptance, its use is not recommended because its synthesis regulation is not representative of mRNA levels. Conclusion Based on geNorm algorithm, we propose the use of three genes SF3A1, EEF1A2 and HMBS as references for normalization of real-time RTqPCR in muscle expression studies.

Practical Challenges for Digital Watermarking Applications

Directory of Open Access Journals (Sweden)

Sharma Ravi K

2002-01-01

Full Text Available The field of digital watermarking has recently seen numerous articles covering novel techniques, theoretical studies, attacks, and analysis. In this paper, we focus on an emerging application to highlight practical challenges for digital watermarking applications. Challenges include design considerations, requirements analysis, choice of watermarking techniques, speed, robustness, and the tradeoffs involved. We describe common attributes of watermarking systems and discuss the challenges in developing real world applications. Our application uses digital watermarking to connect ordinary toys to the digital world. The application captures important aspects of watermarking systems and illustrates some of the design issues faced.

Differentially expressed proteins among normal cervix, cervical intraepithelial neoplasia and cervical squamous cell carcinoma.

Science.gov (United States)

Zhao, Q; He, Y; Wang, X-L; Zhang, Y-X; Wu, Y-M

2015-08-01

To explore the differentially expressed proteins in normal cervix, cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC) tissues by differential proteomics technique. Cervical tissues (including normal cervix, CIN and CSCC) were collected in Department of Gynecologic Oncology of Beijing Obstetrics and Gynecology Hospital. Two-dimensional fluorescence difference in gel electrophoresis (2-D DIGE) and DeCyder software were used to detect the differentially expressed proteins. Matrix-assisted laser desorption/ionization-time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS) was used to identify the differentially expressed proteins. Western blot (WB) and immunohistochemistry (IHC) were performed to validate the expressions of selected proteins among normal cervix, CIN and CSCC. 2-D DIGE images with high resolution and good repeatability were obtained. Forty-six differentially expressed proteins (27 up-regulated and 19 down-regulated) were differentially expressed among the normal cervix, CIN and CSCC. 26 proteins were successfully identified by MALDI-TOF/TOF MS. S100A9 (S100 calcium-binding protein A9) was the most significantly up-regulated protein. Eukaryotic elongation factor 1-alpha-1 (eEF1A1) was the most significantly down-regulated protein. Pyruvate kinase isozymes M2 (PKM2) was both up-regulated and down-regulated. The results of WB showed that with the increase in the severity of cervical lesions, the expression of S100A9 protein was significantly increased among the three groups (P = 0.010). The expression of eEF1A1 was reduced but without significant difference (P = 0.861). The expression of PKM2 was significantly reduced (P = 0.000). IHC showed that protein S100A9 was mainly expressed in the cytoplasm, and its positive expression rate was 20.0 % in normal cervix, 70.0 % in CIN and 100.0 % in CSCC, with a significant difference among them (P = 0.006). eEF1A1 was mainly expressed in the cell plasma, and its

6-Electron exchange function as a simple estimator of aromaticity in large polyaromatic hydrocarbons

Science.gov (United States)

Mandado, Marcos; Mosquera, Ricardo A.

2009-02-01

The 6-electron exchange function (6-EEF) is defined and calculated for a series of large polyaromatic hydrocarbons (PAHs). It is shown that the 6-EEF, computed at selected points in space, is able to reproduce in PAHs the same relative values as the multicenter electron delocalization indices with an affordable computational cost and without using any definition of the atom in the molecule. Calculations for a series of D 6h PAHs ranging from C 6H 6 to C 216H 36 are performed. The results can be extrapolated to even larger PAHs and allow predicting the behaviour of a benzene ring in an infinite sheet of graphite.

Nonstationary Hydrological Frequency Analysis: Theoretical Methods and Application Challenges

Science.gov (United States)

Xiong, L.

2014-12-01

Because of its great implications in the design and operation of hydraulic structures under changing environments (either climate change or anthropogenic changes), nonstationary hydrological frequency analysis has become so important and essential. Two important achievements have been made in methods. Without adhering to the consistency assumption in the traditional hydrological frequency analysis, the time-varying probability distribution of any hydrological variable can be established by linking the distribution parameters to some covariates such as time or physical variables with the help of some powerful tools like the Generalized Additive Model of Location, Scale and Shape (GAMLSS). With the help of copulas, the multivariate nonstationary hydrological frequency analysis has also become feasible. However, applications of the nonstationary hydrological frequency formula to the design and operation of hydraulic structures for coping with the impacts of changing environments in practice is still faced with many challenges. First, the nonstationary hydrological frequency formulae with time as covariate could only be extrapolated for a very short time period beyond the latest observation time, because such kind of formulae is not physically constrained and the extrapolated outcomes could be unrealistic. There are two physically reasonable methods that can be used for changing environments, one is to directly link the quantiles or the distribution parameters to some measureable physical factors, and the other is to use the derived probability distributions based on hydrological processes. However, both methods are with a certain degree of uncertainty. For the design and operation of hydraulic structures under changing environments, it is recommended that design results of both stationary and nonstationary methods be presented together and compared with each other, to help us understand the potential risks of each method.

An interview-informed synthesized contingency analysis to inform the treatment of challenging behavior in a young child with autism.

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Herman, Ciara; Healy, Olive; Lydon, Sinéad

2018-04-01

Experimental Functional analysis (EFA) is considered the "gold standard" of behavioural assessment and its use is predictive of treatment success. However, EFA has a number of limitations including its lengthy nature, the high level of expertise required, and the reinforcement of challenging behaviour. This study aimed to further validate a novel interview-informed synthesised contingency analysis (IISCA). An open-ended interview and brief direct observation informed an IISCA for a young boy with autism who engaged in challenging behaviour. Resulting data supported the hypothesis that the target behaviour was multiply controlled by escape from demands and access to tangible items. An intervention comprised of most-to-least prompting, escape extinction, differential reinforcement and a high-probability instruction sequence was evaluated using a reversal design. This intervention reduced challenging behaviour to low levels and resulted in increased compliance. Findings support the status of the IISCA as a valid, practical, and effective process for designing function-based interventions.

Acute and chronic effects of cannabinoids on effort-related decision-making and reward learning: an evaluation of the cannabis 'amotivational' hypotheses.

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Lawn, Will; Freeman, Tom P; Pope, Rebecca A; Joye, Alyssa; Harvey, Lisa; Hindocha, Chandni; Mokrysz, Claire; Moss, Abigail; Wall, Matthew B; Bloomfield, Michael Ap; Das, Ravi K; Morgan, Celia Ja; Nutt, David J; Curran, H Valerie

2016-10-01

Anecdotally, both acute and chronic cannabis use have been associated with apathy, amotivation, and other reward processing deficits. To date, empirical support for these effects is limited, and no previous studies have assessed both acute effects of Δ-9-tetrahydrocannabinol (THC) and cannabidiol (CBD), as well as associations with cannabis dependence. The objectives of this study were (1) to examine acute effects of cannabis with CBD (Cann + CBD) and without CBD (Cann-CBD) on effort-related decision-making and (2) to examine associations between cannabis dependence, effort-related decision-making and reward learning. In study 1, 17 participants each received three acute vaporized treatments, namely Cann-CBD (8 mg THC), Cann + CBD (8 mg THC + 10 mg CBD) and matched placebo, followed by a 50 % dose top-up 1.5 h later, and completed the Effort Expenditure for Rewards Task (EEfRT). In study 2, 20 cannabis-dependent participants were compared with 20 non-dependent, drug-using control participants on the EEfRT and the Probabilistic Reward Task (PRT) in a non-intoxicated state. Cann-CBD reduced the likelihood of high-effort choices relative to placebo (p = 0.042) and increased sensitivity to expected value compared to both placebo (p = 0.014) and Cann + CBD (p = 0.006). The cannabis-dependent and control groups did not differ on the EEfRT. However, the cannabis-dependent group exhibited a weaker response bias than the control group on the PRT (p = 0.007). Cannabis acutely induced a transient amotivational state and CBD influenced the effects of THC on expected value. In contrast, cannabis dependence was associated with preserved motivation alongside impaired reward learning, although confounding factors, including depression, cannot be disregarded. This is the first well powered, fully controlled study to objectively demonstrate the acute amotivational effects of THC.

Advantages and Challenges of Dried Blood Spot Analysis by Mass Spectrometry Across the Total Testing Process

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Zakaria, Rosita; Allen, Katrina J.; Koplin, Jennifer J.; Roche, Peter

2016-01-01

Introduction Through the introduction of advanced analytical techniques and improved throughput, the scope of dried blood spot testing utilising mass spectrometric methods, has broadly expanded. Clinicians and researchers have become very enthusiastic about the potential applications of dried blood spot based mass spectrometric applications. Analysts on the other hand face challenges of sensitivity, reproducibility and overall accuracy of dried blood spot quantification. In this review, we aim to bring together these two facets to discuss the advantages and current challenges of non-newborn screening applications of dried blood spot quantification by mass spectrometry. Methods To address these aims we performed a key word search of the PubMed and MEDLINE online databases in conjunction with individual manual searches to gather information. Keywords for the initial search included; “blood spot” and “mass spectrometry”; while excluding “newborn”; and “neonate”. In addition, databases were restricted to English language and human specific. There was no time period limit applied. Results As a result of these selection criteria, 194 references were identified for review. For presentation, this information is divided into: 1) clinical applications; and 2) analytical considerations across the total testing process; being pre-analytical, analytical and post-analytical considerations. Conclusions DBS analysis using MS applications is now broadly applied, with drug monitoring for both therapeutic and toxicological analysis being the most extensively reported. Several parameters can affect the accuracy of DBS measurement and further bridge experiments are required to develop adjustment rules for comparability between dried blood spot measures and the equivalent serum/plasma values. Likewise, the establishment of independent reference intervals for dried blood spot sample matrix is required. PMID:28149263

Lessons learned in induced fit docking and metadynamics in the Drug Design Data Resource Grand Challenge 2

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Baumgartner, Matthew P.; Evans, David A.

2018-01-01

Two of the major ongoing challenges in computational drug discovery are predicting the binding pose and affinity of a compound to a protein. The Drug Design Data Resource Grand Challenge 2 was developed to address these problems and to drive development of new methods. The challenge provided the 2D structures of compounds for which the organizers help blinded data in the form of 35 X-ray crystal structures and 102 binding affinity measurements and challenged participants to predict the binding pose and affinity of the compounds. We tested a number of pose prediction methods as part of the challenge; we found that docking methods that incorporate protein flexibility (Induced Fit Docking) outperformed methods that treated the protein as rigid. We also found that using binding pose metadynamics, a molecular dynamics based method, to score docked poses provided the best predictions of our methods with an average RMSD of 2.01 Å. We tested both structure-based (e.g. docking) and ligand-based methods (e.g. QSAR) in the affinity prediction portion of the competition. We found that our structure-based methods based on docking with Smina (Spearman ρ = 0.614), performed slightly better than our ligand-based methods (ρ = 0.543), and had equivalent performance with the other top methods in the competition. Despite the overall good performance of our methods in comparison to other participants in the challenge, there exists significant room for improvement especially in cases such as these where protein flexibility plays such a large role.

Agile text mining for the 2014 i2b2/UTHealth Cardiac risk factors challenge.

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Cormack, James; Nath, Chinmoy; Milward, David; Raja, Kalpana; Jonnalagadda, Siddhartha R

2015-12-01

This paper describes the use of an agile text mining platform (Linguamatics' Interactive Information Extraction Platform, I2E) to extract document-level cardiac risk factors in patient records as defined in the i2b2/UTHealth 2014 challenge. The approach uses a data-driven rule-based methodology with the addition of a simple supervised classifier. We demonstrate that agile text mining allows for rapid optimization of extraction strategies, while post-processing can leverage annotation guidelines, corpus statistics and logic inferred from the gold standard data. We also show how data imbalance in a training set affects performance. Evaluation of this approach on the test data gave an F-Score of 91.7%, one percent behind the top performing system. Copyright © 2015 Elsevier Inc. All rights reserved.

Memory Efficient Sequence Analysis Using Compressed Data Structures (Metagenomics Informatics Challenges Workshop: 10K Genomes at a Time)

Energy Technology Data Exchange (ETDEWEB)

Simpson, Jared

2011-10-13

Wellcome Trust Sanger Institute's Jared Simpson on Memory efficient sequence analysis using compressed data structures at the Metagenomics Informatics Challenges Workshop held at the DOE JGI on October 12-13, 2011.

Finding the Words: Medical Students' Reflections on Communication Challenges in Clinic.

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Braverman, Genna; Bereknyei Merrell, Sylvia; Bruce, Janine S; Makoul, Gregory; Schillinger, Erika

2016-11-01

Interpersonal communication is essential to providing excellent patient care and requires ongoing development. Although aspects of medical student interpersonal communication may degrade throughout career progression, it is unknown what specific elements pose challenges. We aimed to characterize clerkship students' perspectives on communication challenges in the outpatient setting to help inform curricular development. Third-year medical students in a required family medicine clerkship were asked to describe a communication challenge they encountered. Open-ended written responses were collected through a mandatory post-clerkship survey. Responses were qualitatively coded using an a priori framework for teaching and assessing communication skills (The SEGUE Framework for Teaching and Assessing Communication Skills) with data-derived additions to the framework, followed by a team-based thematic analysis. We collected 799 reflections written by 518 students from 2007-2014. Three dominant themes emerged from the analysis: challenges with (1) effectively exchanging information with patients, (2) managing emotional aspects of the patient encounter, and (3) negotiating terms of the encounter. Communication curricula focus on content and process of the medical interview, but insufficient time and energy are devoted to psychosocial factors, including aspects of the encounter that are emotionally charged or conflicting. While gaps in students' communication skillsets may be anticipated or observed by educators, this study offers an analysis of students' own perceptions of the challenges they face.

Evaluation of olopatadine 0.2% in the complete prevention of ocular itching in the conjunctival allergen challenge model

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Kabat AG

2011-07-01

Full Text Available Alan G Kabat1, David B Granet2, Dina Amin3, Maria J Tort3, Michael S Blaiss41Nova Southeastern University, Fort Lauderdale, FL, USA; 2Shiley Eye Center, University of California San Diego, La Jolla, CA, USA; 3Alcon Research Ltd, Fort Worth, TX, USA; 4University of Tennessee Health Science Center, Memphis, TN, USABackground: Olopatadine hydrochloride ophthalmic solution 0.2% (PATADAY™; Alcon, Fort Worth, TX (olopatadine 0.2% is a formulation of a multi-action agent that has been approved for the treatment of ocular itching associated with allergic conjunctivitis when used once daily.Objective: To evaluate olopatadine 0.2% versus its vehicle in the complete prevention of ocular itching in adult patients with allergic conjunctivitis.Methods: This paper presents a post-hoc analysis of subgroup results from a single-center, double-masked, randomized, contralateral eye, conjunctival allergen challenge study. The post-hoc efficacy analysis, conducted with data from patients who instilled olopatadine 0.2% in a single eye and vehicle in the contralateral eye, compared the ability of each study formulation to completely prevent ocular itching at three assessment time points post-instillation. Safety was not reevaluated in the post-hoc analysis.Results: Overall, 40 patients received contralateral instillations of study drug and were included in the post-hoc analysis. At all three post-instillation time points, significantly greater proportions of patients reported itching scores of 0 in the olopatadine 0.2%-treated eye than in the vehicle-treated eye (63%–65% versus 3%–10%, respectively; P < 0.05 for each comparison. Within the previously reported results for the full study, no clinically relevant or statistically significant changes from baseline were observed for patients in regard to visual acuity, ocular signs, or fundus parameters.Conclusion: Olopatadine 0.2% is safe, well tolerated, and superior to vehicle in completely preventing ocular

Evaluation and Analysis of Eco-Security in Environmentally Sensitive Areas Using an Emergy Ecological Footprint

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Han-Shen Chen

2017-01-01

Full Text Available In this paper, the overall ecological and environmental sustainability in the Cing-Jing region in Taiwan is examined. As land use and cover change has been found to be an important analysis method, an emergy ecological footprint model was applied and the eco-security assessed to ensure authorities maintain a balance between ecological preservation and tourism development. While the ecological environment in the Cing-Jing region from 2008 to 2014 was found to be within safe levels, all related indices had increased considerably. A Grey model was used to predict the 2015–2024 ecological carrying capacities, from which it was found that there is expected to be a large increase in per capita ecological footprints (EFs, meaning that in the future there is going to be a larger ecological deficit and a higher ecological pressure index (EFI, with the eco-security predicted to reach a Grade 2 intermediate level in 2022. As the Cing-Jing region is predicted to become ecologically unsustainable, local, regional, and national governments need to implement regulations to strictly control the land use in the Cing-Jing region. This study demonstrated that emergy EF (EEF theory application can give objective guidance to decision-makers to ensure that recreational non-urban eco-security can be maintained at a safe level.

Evaluation and Analysis of Eco-Security in Environmentally Sensitive Areas Using an Emergy Ecological Footprint

Science.gov (United States)

Chen, Han-Shen

2017-01-01

In this paper, the overall ecological and environmental sustainability in the Cing-Jing region in Taiwan is examined. As land use and cover change has been found to be an important analysis method, an emergy ecological footprint model was applied and the eco-security assessed to ensure authorities maintain a balance between ecological preservation and tourism development. While the ecological environment in the Cing-Jing region from 2008 to 2014 was found to be within safe levels, all related indices had increased considerably. A Grey model was used to predict the 2015–2024 ecological carrying capacities, from which it was found that there is expected to be a large increase in per capita ecological footprints (EFs), meaning that in the future there is going to be a larger ecological deficit and a higher ecological pressure index (EFI), with the eco-security predicted to reach a Grade 2 intermediate level in 2022. As the Cing-Jing region is predicted to become ecologically unsustainable, local, regional, and national governments need to implement regulations to strictly control the land use in the Cing-Jing region. This study demonstrated that emergy EF (EEF) theory application can give objective guidance to decision-makers to ensure that recreational non-urban eco-security can be maintained at a safe level. PMID:28146086

Multimedia content analysis, management and retrieval: trends and challenges

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Hanjalic, Alan; Sebe, Nicu; Chang, Edward

2006-01-01

Recent advances in computing, communications and storage technology have made multimedia data become prevalent. Multimedia has gained enormous potential in improving the processes in a wide range of fields, such as advertising and marketing, education and training, entertainment, medicine, surveillance, wearable computing, biometrics, and remote sensing. Rich content of multimedia data, built through the synergies of the information contained in different modalities, calls for new and innovative methods for modeling, processing, mining, organizing, and indexing of this data for effective and efficient searching, retrieval, delivery, management and sharing of multimedia content, as required by the applications in the abovementioned fields. The objective of this paper is to present our views on the trends that should be followed when developing such methods, to elaborate on the related research challenges, and to introduce the new conference, Multimedia Content Analysis, Management and Retrieval, as a premium venue for presenting and discussing these methods with the scientific community. Starting from 2006, the conference will be held annually as a part of the IS&T/SPIE Electronic Imaging event.

Status of selected ion flow tube MS: accomplishments and challenges in breath analysis and other areas.

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Smith, David; Španěl, Patrik

2016-06-01

This article reflects our observations of recent accomplishments made using selected ion flow tube MS (SIFT-MS). Only brief descriptions are given of SIFT-MS as an analytical method and of the recent extensions to the underpinning analytical ion chemistry required to realize more robust analyses. The challenge of breath analysis is given special attention because, when achieved, it renders analysis of other air media relatively straightforward. Brief overviews are given of recent SIFT-MS breath analyses by leading research groups, noting the desirability of detection and quantification of single volatile biomarkers rather than reliance on statistical analyses, if breath analysis is to be accepted into clinical practice. A 'strengths, weaknesses, opportunities and threats' analysis of SIFT-MS is made, which should help to increase its utility for trace gas analysis.

Analysis of Solid Waste Management Logistics and Its Attendant Challenges in Lagos Metropolis

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Boye Benedict Ayantoyinbo

2018-06-01

Full Text Available This study examined the relationship between waste management logistics and identified metrics for waste management logistics performance. Secondly, the study assessed the various challenges inhibiting the performance of LAWMA in the State. Random table sampling and purposive sampling were used to select 47 waste collection centres with 10 questionnaires distributed per centre (470 in total across the 20 Local Government Areas (LGA in Lagos State. However, only 339 questionnaires were retrieved from the sampled population. Multiple regression analysis was used to predict the relationship between waste management logistics and identified metrics for waste logistics performance. Descriptive statistics was used to explain the challenges of the Lagos State Waste Management Authority (LAWMA. The results established that the volume of solid waste and commitment of staff are crucial to waste management logistics and one factor that strongly affects waste logistics is traffic in the metropolis. Conclusively, waste collection turnaround must be increased and government and private investors should provide enabling infrastructure and trained personnel for effective solid waste management in Lagos metropolis.

Pumpkin eIF5A isoforms interact with components of the translational machinery in the cucurbit sieve tube system.

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Ma, Yi; Miura, Eriko; Ham, Byung-Kook; Cheng, Hao-Wen; Lee, Young-Jin; Lucas, William J

2010-11-01

In yeast, eIF5A, in combination with eEF2, functions at the translation step, during the protein elongation cycle. This result is of significance with respect to functioning of the enucleate sieve tube system, as eIF5A was recently detected in Cucurbita maxima (pumpkin) phloem sap. In the present study, we further characterized four CmeIF5A isoforms, encoding three proteins, all of which were present in the phloem sap. Although hypusination of CmeIF5A was not necessary for entry into the sieve elements, this unique post-translational modification was necessary for RNA binding. The two enzymes required for hypusination were detected in pumpkin phloem sap, where presumably this modification takes place. A combination of gel-filtration chromatography and protein overlay assays demonstrated that, as in yeast, CmeIF5A interacts with phloem proteins, like eEF2, known to be involved in protein synthesis. These findings are discussed in terms of a potential role for eIF5A in regulating protein synthesis within the enucleate sieve tube system of the angiosperms. © 2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd.

Transcriptome analysis of grey mullet (Mugil cephalus) after challenge with Lactococcus garvieae.

Science.gov (United States)

Byadgi, Omkar; Chen, Yao-Chung; Barnes, Andrew C; Tsai, Ming-An; Wang, Pei-Chyi; Chen, Shih-Chu

2016-11-01

Grey mullet (Mugil cephalus) is an economically important fish species in Taiwan mariculture industry. Moreover, grey mullet are common hosts of a bacterial infection by Lactococcus garvieae. However, until now the information related to the immune system of grey mullet is unclear. Therefore, to understand the molecular basis underlying the host immune response to L. garvieae infection, Illumina HiSeq™ 2000 was used to analyse the head kidney and spleen transcriptome of infected grey mullet. De novo assembly of paired-end reads yielded 55,203 unigenes. Comparative analysis of the expression profiles between bacterial challenge fish and control fish identified a total of 7192 from head kidney and 7280 in spleen differentially expressed genes (P grey mullet to Lactococcus garvieae, carrying out detailed functional analysis of these genes and developing strategies for efficient immune protection against infections in grey mullet. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

Optimal therapy of type 2 diabetes: a controversial challenge

Science.gov (United States)

Dardano, Angela; Penno, Giuseppe; Del Prato, Stefano; Miccoli, Roberto

2014-01-01

Type 2 diabetes mellitus (T2DM) is one of the most common chronic disorders in older adults and the number of elderly diabetic subjects is growing worldwide. Nonetheless, the diagnosis of T2DM in elderly population is often missed or delayed until an acute metabolic emergency occurs. Accumulating evidence suggests that both aging and environmental factors contribute to the high prevalence of diabetes in the elderly. Clinical management of T2DM in elderly subjects presents unique challenges because of the multifaceted geriatric scenario. Diabetes significantly lowers the chances of “successful” aging, notably it increases functional limitations and impairs quality of life. In this regard, older diabetic patients have a high burden of comorbidities, diabetes-related complications, physical disability, cognitive impairment and malnutrition, and they are more susceptible to the complications of dysglycemia and polypharmacy. Several national and international organizations have delivered guidelines to implement optimal therapy in older diabetic patients based on individualized treatment goals. This means appreciation of the heterogeneity of the disease as generated by life expectancy, functional reserve, social support, as well as personal preference. This paper will review current treatments for achieving glycemic targets in elderly diabetic patients, and discuss the potential role of emerging treatments in this patient population. PMID:24753144

THE CHALLENGES OF E-GOVERNMENT 2.0 PROJECTS IN ROMANIA: AN INSIGHT

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DIDRAGA OTNIEL

2015-08-01

Full Text Available E-government has developed rapidly and consistently along with the development of ICT. Providing reliable eservices resulting from successful e-government projects means tackling different challenges like transparency and accessibility, technological infrastructure interoperability, end-user adoption, citizen privacy, security and trust, policy updating, and organizational transformation. E-government 2.0 means innovation, transformation, communication, transparency, collaboration and participation, less bureaucracy, and less corruption. Also, investments in egovernment 2.0 projects in Romania must meet the requirements of the strategic lines of development in the National Strategy on Digital Agenda for Romania.

Anticholinesterase activity and chemical profile of an active chromatographic fraction of ethanolic extract from Bellis perennis L. (Asteraceae) flowers

International Nuclear Information System (INIS)

Marques, Thiago Henrique Costa; Santos, Pauline Sousa dos; Freitas, Rivelilson Mendes de; Carvalho, Rusbene Bruno Fonseca de; Melo, Cassio Herbert Santos de; David, Juceni Pereira; David, Jorge Mauricio; Lima, Luciano Silva

2013-01-01

This work describes the isolation of an active flavonoid fraction and identification of isorhamnetin 3-O-β-D-(6’’-acetyl)- alactopyranoside from flowers of B. perennis, and also the evaluation of anticholinesterase (AChE) activity of ethanolic extract from flowers (EEF) and the active fraction. The chemical structure of the flavonoid was defined on the basis of spectroscopic 1 H NMR, IR and UV data. EEF or flavonoid reduces AChE activity in vivo, while flavonoid also reduces AChE activity in vitro, showing a value of 1.49 μM for 50% inhibitory concentration (IC 50 ), suggesting potential use as an insecticide or in the treatment of neurodegenerative diseases such as Alzheimer’s disease. (author)

The District Health Information System (DHIS2): A literature review and meta-synthesis of its strengths and operational challenges based on the experiences of 11 countries.

Science.gov (United States)

Dehnavieh, Reza; Haghdoost, AliAkbar; Khosravi, Ardeshir; Hoseinabadi, Fahime; Rahimi, Hamed; Poursheikhali, Atousa; Khajehpour, Nahid; Khajeh, Zahra; Mirshekari, Nadia; Hasani, Marziyeh; Radmerikhi, Samera; Haghighi, Hajar; Mehrolhassani, Mohammad Hossain; Kazemi, Elaheh; Aghamohamadi, Saeide

2018-01-01

Health information systems offer many potential benefits for healthcare, including financial benefits and for improving the quality of patient care. The purpose of District Health Information Systems (DHIS) is to document data that are routinely collected in all public health facilities in a country using the system. The aim of this study was to examine the strengths and operational challenges of DHIS2, with a goal to enable decision makers in different counties to more accurately evaluate the outcomes of introducing DHIS2 into their particular country. A review of the literature combined with the method of meta-synthesis was used to source information and interpret results relating to the strengths and operational challenges of DHIS2. Databases (Embase, PubMed, Scopus and Google Scholar) were searched for documents related to strengths and operational challenges of DHIS2, with no time limit up to 8 April 2017. The review and evaluation of selected studies was conducted in three stages: title, abstract and full text. Each of the selected studies was reviewed carefully and key concepts extracted. These key concepts were divided into two categories of strengths and operational challenges of DHIS2. Then, each category was grouped based on conceptual similarity to achieve the main themes and sub-themes. Content analysis was used to analyse extracted data. Of 766 identified citations, 20 studies from 11 countries were included and analysed in this study. Identified strengths in the DHIS were represented in seven themes (with 21 categories): technical features of software, proper management of data, application flexibility, networking and increasing the satisfaction of stakeholders, development of data management, increasing access to information and economic benefits. Operational challenges were identified and captured in 11 themes (with 18 categories): funds; appropriate communication infrastructure; the need for the existence of appropriate data; political, cultural

Real SQL queries 50 challenges : practice for reporting and analysis

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Cohen, Brian; Mishra, Neerja

2015-01-01

Queries improve when challenges are authentic. This book sets your learning on the fast track with realistic problems to solve. Topics span sales, marketing, human resources, purchasing, and production. Real SQL Queries: 50 Challenges is perfect for analysts, report writers, or anyone searching for a hands-on approach to learning SQL Server.

Response of runoff and soil loss to reforestation and rainfall type in red soil region of southern China.

Science.gov (United States)

Huang, Zhigang; Ouyang, Zhiyun; Li, Fengrui; Zheng, Hua; Wang, Xiaoke

2010-01-01

To evaluate the long-term effects of reforestation types on soil erosion on degraded land, vegetation and soil properties under conventional sloping farmland (CSF) and three different reforestation types including a Pinus massoniana secondary forest (PSF), an Eucommia ulmoides artificial economic forest (EEF) and a natural succession type forest (NST), were investigated at runoff plot scale over a six-year period in a red soil region of southern China. One hundred and thirty erosive rainfall events generating runoff in plots were grouped into four rainfall types by means of K-mean clustering method. Erosive rainfall type I is the dominant rainfall type. The amount of runoff and the soil loss under erosive rainfall type III were the most, followed by rain-fall type II, IV and I. Compared with CSF treatment, reforestation treatments decreased the average annual runoff depth and the soil loss by 25.5%-61.8% and 93.9%-96.2% during the study period respectively. Meanwhile, runoff depth at PSF and EEF treatments was significantly lower than that in NST treatment, but no significant difference existed in soil erosion modulus among the three reforestation treatments. This is mainly due to the improved vegetation properties (i.e., vegetation coverage, biomass of above- and below-ground and litter-fall mass) and soil properties (i.e., bulk density, total porosity, infiltration rate and organic carbon content) in the three reforestation treatments compared to CSF treatment. The PSF and EEF are recommended as the preferred reforestation types to control runoff and soil erosion in the red soil region of southern China, with the NST potentially being used as an important supplement.

Application of Entropy Ensemble Filter in Neural Network Forecasts of Tropical Pacific Sea Surface Temperatures

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Hossein Foroozand

2018-03-01

Full Text Available Recently, the Entropy Ensemble Filter (EEF method was proposed to mitigate the computational cost of the Bootstrap AGGregatING (bagging method. This method uses the most informative training data sets in the model ensemble rather than all ensemble members created by the conventional bagging. In this study, we evaluate, for the first time, the application of the EEF method in Neural Network (NN modeling of El Nino-southern oscillation. Specifically, we forecast the first five principal components (PCs of sea surface temperature monthly anomaly fields over tropical Pacific, at different lead times (from 3 to 15 months, with a three-month increment for the period 1979–2017. We apply the EEF method in a multiple-linear regression (MLR model and two NN models, one using Bayesian regularization and one Levenberg-Marquardt algorithm for training, and evaluate their performance and computational efficiency relative to the same models with conventional bagging. All models perform equally well at the lead time of 3 and 6 months, while at higher lead times, the MLR model’s skill deteriorates faster than the nonlinear models. The neural network models with both bagging methods produce equally successful forecasts with the same computational efficiency. It remains to be shown whether this finding is sensitive to the dataset size.

Purity and radioactive decay behaviour of industrial 2D-reinforced SiCf/SiC composites

International Nuclear Information System (INIS)

Scholz, H.W.; Zucchetti, M.; Casteleyn, K.; Adelhelm, C.

1994-01-01

Ceramic matrix composites based on SiC with continuous fibres (SiC f /SiC) are considered promising structural materials for future fusion devices. It was still to clarify, whether impurities in industrial SiC f /SiC could jeopardise radiological advantages. Experimental impurity analyses revealed a two-dimensionally reinforced SiC f /SiC with the matrix produced by CVI as very pure. Chemo-spectrometric methods were combined with radioactivation methods (CPAA, NAA). A quantification of the main constituents Si, C and O was added. Calculations with the FISPACT-2.4 code and EAF-2 library identified elements detrimental for different low-activation criteria. For the neutron exposure, EEF reactor-study first wall and blanket conditions were simulated. The calculated SiC f /SiC included 48 trace elements. Even under conservative assumptions, all low-activation limits of European interest are fulfilled. Exclusively the hands-on recycling limit for the First Wall can intrinsically not be satisfied with SiC. The theoretical goal of a SiC f /SiC depleted of 28 Si (isotopic tailoring) is critically discussed. ((orig.))

A cost-benefit analysis of alternatively fueled buses with special considerations for V2G technology

International Nuclear Information System (INIS)

Shirazi, Yosef; Carr, Edward; Knapp, Lauren

2015-01-01

Motivated by climate, health and economic considerations, alternatively-fueled bus fleets have emerged worldwide. Two popular alternatives are compressed natural gas (CNG) and electric vehicles. The latter provides the opportunity to generate revenue through vehicle-to-grid (V2G) services if properly equipped. This analysis conducts a robust accounting of the costs of diesel, CNG and battery-electric powertrains for school buses. Both marginal and fleet-wide scenarios are explored. Results indicate that the marginal addition of neither a small CNG nor a small V2G-enabled electric bus is cost effective at current prices. Contrary to previous findings, a small V2G-enabled electric bus increases net present costs by $7,200/seat relative to diesel for a Philadelphia, PA school district. A small CNG bus increases costs by $1,200/seat relative to diesel. This analysis is the first to quantify and include the economic implications of cold temperature extremes on electric vehicle battery operations, and the lower V2G revenues that result. Additional costs and limitations imposed by electric vehicles performing V2G are frequently overlooked in the literature and are explored here. If a variety of technical, legal, and economic challenges are overcome, a future eBus may be economical. - Highlights: • We present a robust cost-benefit analysis of various bus technologies. • Diesel is a low-cost technology at current prices. • CNG represents slightly higher costs on a marginal bus basis. • V2G-enabled electric buses are not cost-effective at current prices. • We identify frequently overlooked costs and challenges to V2G implementation.

Analysis of MicroRNA Expression Profiles in Weaned Pig Skeletal Muscle after Lipopolysaccharide Challenge

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Jing Zhang

2015-09-01

Full Text Available MicroRNAs (miRNAs constitute a class of non-coding RNAs that play a crucial regulatory role in skeletal muscle development and disease. Several acute inflammation conditions including sepsis and cancer are characterized by a loss of skeletal muscle due primarily to excessive muscle catabolism. As a well-known inducer of acute inflammation, a lipopolysaccharide (LPS challenge can cause serious skeletal muscle wasting. However, knowledge of the role of miRNAs in the course of inflammatory muscle catabolism is still very limited. In this study, RNA extracted from the skeletal muscle of pigs injected with LPS or saline was subjected to small RNA deep sequencing. We identified 304 conserved and 114 novel candidate miRNAs in the pig. Of these, four were significantly increased in the LPS-challenged samples and five were decreased. The expression of five miRNAs (ssc-miR-146a-5p, ssc-miR-221-5p, ssc-miR-148b-3p, ssc-miR-215 and ssc-miR-192 were selected for validation by quantitative polymerase chain reaction (qPCR, which found that ssc-miR-146a-5p and ssc-miR-221-5p were significantly upregulated in LPS-challenged pig skeletal muscle. Moreover, we treated mouse C2C12 myotubes with 1000 ng/mL LPS as an acute inflammation cell model. Expression of TNF-α, IL-6, muscle atrophy F-box (MAFbx and muscle RING finger 1 (MuRF1 mRNA was strongly induced by LPS. Importantly, miR-146a-5p and miR-221-5p also showed markedly increased expression in LPS-treated C2C12 myotubes, suggesting the two miRNAs may be involved in muscle catabolism systems in response to acute inflammation caused by a LPS challenge. To our knowledge, this study is the first to examine miRNA expression profiles in weaned pig skeletal muscle challenged with LPS, and furthers our understanding of miRNA function in the regulation of inflammatory muscle catabolism.

Educational Challenges to Train Accountable Graduates

Directory of Open Access Journals (Sweden)

Mohamadreza Abdolmaleki

2017-04-01

Full Text Available Background and Purpose: social accountability, a concept which is in the focal attention more than ever, is to provide service in the field of medical sciences. We aimed to identify the educational challenges to train accountable graduates in the medical education system to meet social needs.Methods: This study was conducted by qualitative content analysis using in-depth semi-structured interviews with eleven academic members of Kurdistan University of Medical Sciences who were selected by purposeful sampling. The interviews were analyzed using thematic content analysis.Results: The findings of the study consisted of 3 main themes and each one was extracted from categories, sub-categories, and codes. One of the themes was educational program which consisted of 2 categories called defects in the curriculum and inappropriate educational strategies. The second theme was management policies, including macro policies and the policies of the university. The third theme was personal factors which mostly referred to formal and informal education prior to university.Conclusions: The results of the study indicated the educational challenges to train accountable graduates in the medical education system. It seemed that although the results were obtained from Kurdistan University of Medical Sciences, it had many common points with other universities. Therefore, planning and taking appropriate measures to address these challenges can find a way to train accountable graduates in the medical education system to meet social needs.Keywords: SOCIAL ACCOUNTABILITY, EDUCATIONAL SYSTEM, MEDICAL EDUCATION

Scalability of Comparative Analysis, Novel Algorithms and Tools (MICW - Metagenomics Informatics Challenges Workshop: 10K Genomes at a Time)

Energy Technology Data Exchange (ETDEWEB)

Mavrommatis, Kostas

2011-10-12

DOE JGI's Kostas Mavrommatis, chair of the Scalability of Comparative Analysis, Novel Algorithms and Tools panel, at the Metagenomics Informatics Challenges Workshop held at the DOE JGI on October 12-13, 2011.

Recent progress and challenges in nanotechnology for biomedical applications: an insight into the analysis of neurotransmitters.

Science.gov (United States)

Shankaran, Dhesingh Ravi; Miura, Norio

2007-01-01

Nanotechnology offers exciting opportunities and unprecedented compatibilities in manipulating chemical and biological materials at the atomic or molecular scale for the development of novel functional materials with enhanced capabilities. It plays a central role in the recent technological advances in biomedical technology, especially in the areas of disease diagnosis, drug design and drug delivery. In this review, we present the recent trend and challenges in the development of nanomaterials for biomedical applications with a special emphasis on the analysis of neurotransmitters. Neurotransmitters are the chemical messengers which transform information and signals all over the body. They play prime role in functioning of the central nervous system (CNS) and governs most of the metabolic functions including movement, pleasure, pain, mood, emotion, thinking, digestion, sleep, addiction, fear, anxiety and depression. Thus, development of high-performance and user-friendly analytical methods for ultra-sensitive detection of neurotransmitters remain a major challenge in modern biomedical analysis. Nanostructured materials are emerging as a powerful mean for diagnosis of CNS disorders because of their unique optical, size and surface characteristics. This review provides a brief outline on the basic concepts and recent advancements of nanotechnology for biomedical applications, especially in the analysis of neurotransmitters. A brief introduction to the nanomaterials, bionanotechnology and neurotransmitters is also included along with discussions on most of the patents published in these areas.

Challenges of teacher-based clinical evaluation from nursing students' point of view: Qualitative content analysis.

Science.gov (United States)

Sadeghi, Tabandeh; Seyed Bagheri, Seyed Hamid

2017-01-01

Clinical evaluation is very important in the educational system of nursing. One of the most common methods of clinical evaluation is evaluation by the teacher, but the challenges that students would face in this evaluation method, have not been mentioned. Thus, this study aimed to explore the experiences and views of nursing students about the challenges of teacher-based clinical evaluation. This study was a descriptive qualitative study with a qualitative content analysis approach. Data were gathered through semi-structured focused group sessions with undergraduate nursing students who were passing their 8 th semester at Rafsanjan University of Medical Sciences. Date were analyzed using Graneheim and Lundman's proposed method. Data collection and analysis were concurrent. According to the findings, "factitious evaluation" was the main theme of study that consisted of three categories: "Personal preferences," "unfairness" and "shirking responsibility." These categories are explained using quotes derived from the data. According to the results of this study, teacher-based clinical evaluation would lead to factitious evaluation. Thus, changing this approach of evaluation toward modern methods of evaluation is suggested. The finding can help nursing instructors to get a better understanding of the nursing students' point of view toward this evaluation approach and as a result could be planning for changing of this approach.

THE NIGERIAN GAS MASTER-PLAN, INVESTMENT OPPORTUNITIES, CHALLENGES, ISSUES AFFECTING POWER SECTOR: AN ANALYSIS

Directory of Open Access Journals (Sweden)

R. INGWE

2014-11-01

Full Text Available The Nigerian Gas Master-Plan, Investment Opportunities, Challenges, Issues Affecting Power Sector: an Analysis. The objective of this article is to contribute towards understanding of the Nigerian Gas Master Plan (NGMP/Plan and its bifurcations with key socio-economic development factors. I applied the method of discourse to bring to being some points that have hitherto been unknown about the Master-plan and its inter-relationships and bifurcations. Elaborated here are the spectacular gains that have accrued to the Latin American country, Trinidad and Tobago, from its recent development of natural gas resources. This was considered suitable and significant here for highlighting that if such spectacular achievements could be realized from Trinidad and Tobago’s relatively smaller gas deposit (15.3 tcf, probable reserves (8.4 tcf, possible reserves (6.2 tcf would be by far greater considering Nigeria’s larger natural gas reserves (184 tcf wealth as earlier stated. I show that the Plan is well designed relevant to addressing Nigeria’s current development needs generally. It presents potentials for stimulating Nigeria’s economic growth by harnessing the country’s abundant natural gas reserves. The Plan enumerates/ elaborates huge investment opportunities. Some challenges likely to be faced in the implementation/management of the Plan are already being surmounted as recent reports show that some of its key investments have been realized and the required infrastructure are being provided. Regarding the issues in the Master-plan that are likely to affect and are affecting Nigeria’s power sector development, I reckon that they are mostly positive factors due to the way the plan promises to stimulate electricity generation in our country.

Embracing the challenge of competition

International Nuclear Information System (INIS)

MacDonald, C.

1998-01-01

The new and creative solutions that Edmonton Power is using to meet the challenges of deregulation, competition and demand for customer choice in the electric power industry are discussed. Among these creative initiatives, in 1997 Edmonton Power extended account management services to 850 accounts to deliver commercial and operational analysis of energy consumption and management. Another major focus of effort has been the introduction of time of use (TOU) pricing options to commercial and industrial customers above 150 kWh. This required the installation of the latest hourly metering technology and the establishment of new price plans and contracts. Two major challenges were highlighted: (1) the mismatch between the prices utilities pay at the power pool and what the customer pays at the meter, and (2) the increased volatility of hour to hour price changes are just some of the risks that will have to be resolved under the new market structure

Searching for massive black hole binaries in the first Mock LISA Data Challenge

International Nuclear Information System (INIS)

Cornish, Neil J; Porter, Edward K

2007-01-01

The Mock LISA Data Challenge is a worldwide effort to solve the LISA data analysis problem. We present here our results for the massive black hole binary (BBH) section of round 1. Our results cover challenge 1.2.1, where the coalescence of the binary is seen, and challenge 1.2.2, where the coalescence occurs after the simulated observational period. The data stream is composed of Gaussian instrumental noise plus an unknown BBH waveform. Our search algorithm is based on a variant of the Markov chain Monte Carlo method that uses Metropolis-Hastings sampling and thermostated frequency annealing. We present results from the training data sets where we know the parameter values a priori and the blind data sets where we were informed of the parameter values after the challenge had finished. We demonstrate that our algorithm is able to rapidly locate the sources, accurately recover the source parameters and provide error estimates for the recovered parameters

Progress and challenges of engineering a biophysical CO2-concentrating mechanism into higher plants.

Science.gov (United States)

Rae, Benjamin D; Long, Benedict M; Förster, Britta; Nguyen, Nghiem D; Velanis, Christos N; Atkinson, Nicky; Hee, Wei Yih; Mukherjee, Bratati; Price, G Dean; McCormick, Alistair J

2017-06-01

Growth and productivity in important crop plants is limited by the inefficiencies of the C3 photosynthetic pathway. Introducing CO2-concentrating mechanisms (CCMs) into C3 plants could overcome these limitations and lead to increased yields. Many unicellular microautotrophs, such as cyanobacteria and green algae, possess highly efficient biophysical CCMs that increase CO2 concentrations around the primary carboxylase enzyme, Rubisco, to enhance CO2 assimilation rates. Algal and cyanobacterial CCMs utilize distinct molecular components, but share several functional commonalities. Here we outline the recent progress and current challenges of engineering biophysical CCMs into C3 plants. We review the predicted requirements for a functional biophysical CCM based on current knowledge of cyanobacterial and algal CCMs, the molecular engineering tools and research pipelines required to translate our theoretical knowledge into practice, and the current challenges to achieving these goals. © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

The Impact of Challenging Geometry and Measurement Units on the Achievement of Grade 2 Students

Science.gov (United States)

Gavin, M. Katherine; Casa, Tutita M.; Adelson, Jill L.; Firmender, Janine M.

2013-01-01

The primary goal of Project M[superscript 2] was to develop and field-test challenging geometry and measurement units for all K-2 students. This article reports on the achievement results for students in Grade 2 at 12 urban and suburban sites in 4 states using the Iowa Tests of Basic Skills (ITBS) mathematics concepts subtest and an open-response…

Improved Maneuver Criteria Evaluation Program

Science.gov (United States)

1979-11-01

If the rotor rpm breakpoint (OMGBL2) is le :-s than the mininum rotor rpm (OMEGMN), then the rpm bleed :ate (OMGBDI) will be the only bleed rate used...VCP =60 PSU 1 EEF = 1 OMGBD1=2 OMGBD3=0 OMGRC2=0 VERR = 2 MPRINT= 1 OMEGMN=300 OMGBL.2=4 OMGBL4=0 OMGRD2=0 MUF = 1 BINERT:2860 TRPMMN= 0 OMGBD2=0 OMGBD4...height is within 2 feet of the measured height. These comparisons show that the MCEP maneuvers are accurate for simulating these types of maneuvers

Challenges and solutions for the analysis of in situ, in crystallo micro-spectrophotometric data

International Nuclear Information System (INIS)

Dworkowski, Florian S. N.; Hough, Michael A.; Pompidor, Guillaume; Fuchs, Martin R.

2015-01-01

The particular challenge of the analysis of optical absorption and Raman spectroscopic data measured from protein crystals and how the SLS-APE software toolbox supports scientists in dealing with such data is described. Combining macromolecular crystallography with in crystallo micro-spectrophotometry yields valuable complementary information on the sample, including the redox states of metal cofactors, the identification of bound ligands and the onset and strength of undesired photochemistry, also known as radiation damage. However, the analysis and processing of the resulting data differs significantly from the approaches used for solution spectrophotometric data. The varying size and shape of the sample, together with the suboptimal sample environment, the lack of proper reference signals and the general influence of the X-ray beam on the sample have to be considered and carefully corrected for. In the present article, how to characterize and treat these sample-dependent artefacts in a reproducible manner is discussed and the SLS-APEin situ, in crystallo optical spectroscopy data-analysis toolbox is demonstrated

Comparison of 3 vaccination strategies against porcine reproductive and respiratory syndrome virus, Mycoplasma hyopneumoniae, and porcine circovirus type 2 on a 3 pathogen challenge model.

Science.gov (United States)

Jeong, Jiwoon; Kang, Ikjae; Kim, Seeun; Park, Kee Hwan; Park, Changhoon; Chae, Chanhee

2018-01-01

The objective of this study was to compare clinical, microbiologic, immunologic, and pathologic parameters in pigs each concurrently administered porcine reproductive and respiratory syndrome virus (PRRSV), Mycoplasma hyopneumoniae, and porcine circovirus type 2 (PCV2) vaccine from 1 of 2 commercial sources at 21 days of age and challenged with field strains of each of the 3 pathogens. Pigs were challenged with PRRSV and M. hyopneumoniae at 42 days of age (-14 days post-challenge, dpc) followed by a challenge with PCV2 at 56 days of age (0 dpc). Significant differences were observed between vaccinated challenged and unvaccinated challenged groups in clinical (average daily gain and clinical signs), microbiologic (viremia and nasal shedding), immunologic (antibodies and interferon-γ secreting cells), and pathologic (lesions) outcomes. Significant differences were observed among the 3 vaccinated challenged groups in microbiologic (nasal shedding of M. hyopneumoniae and viremia of PCV2) and immunologic ( M. hyopneumoniae - and PCV2-specific interferon-γ secreting cells) outcomes. The vaccination regimen for PRRSV vaccine, M. hyopneumoniae vaccine, and PCV2 vaccine is efficacious for controlling triple challenge with PRRSV, M. hyopneumoniae, and PCV2 from weaning to finishing period.

Comparison of commercial and experimental porcine circovirus type 2 (PCV2) vaccines using a triple challenge with PCV2, porcine reproductive and respiratory syndrome virus (PRRSV), and porcine parvovirus (PPV).

Science.gov (United States)

Shen, H G; Beach, N M; Huang, Y W; Halbur, P G; Meng, X J; Opriessnig, T

2010-08-23

The efficacies of commercial porcine circovirus type 2 (PCV2) vaccines and a live PCV1-2a chimeric vaccine were compared in conventional, PCV2-positive piglets using a PCV2-porcine reproductive and respiratory syndrome virus (PRRSV)-porcine parvovirus (PPV) coinfection challenge model. Seventy-three, 2-week-old pigs were randomized into seven groups including five vaccinated and two control groups. Pigs in the vaccinated groups were vaccinated at 3 weeks (one dose) or at 3 and 6 weeks (two dose) of age. All vaccine regimens tested were effective in reducing naturally occurring PCV2 viremia at 16 weeks of age and after PCV2 challenge, demonstrating the capability of the products to induce a lasting protective immunity despite the presence of PCV2 viremia at the time of vaccination. Copyright 2010 Elsevier Ltd. All rights reserved.

Heterogeneous electrochemical CO2 reduction using nonmetallic carbon-based catalysts: current status and future challenges

Science.gov (United States)

Ma, Tao; Fan, Qun; Tao, Hengcong; Han, Zishan; Jia, Mingwen; Gao, Yunnan; Ma, Wangjing; Sun, Zhenyu

2017-11-01

Electrochemical CO2 reduction (ECR) offers an important pathway for renewable energy storage and fuels production. It still remains a challenge in designing highly selective, energy-efficient, robust, and cost-effective electrocatalysts to facilitate this kinetically slow process. Metal-free carbon-based materials have features of low cost, good electrical conductivity, renewability, diverse structure, and tunability in surface chemistry. In particular, surface functionalization of carbon materials, for example by doping with heteroatoms, enables access to unique active site architectures for CO2 adsorption and activation, leading to interesting catalytic performances in ECR. We aim to provide a comprehensive review of this category of metal-free catalysts for ECR, providing discussions and/or comparisons among different nonmetallic catalysts, and also possible origin of catalytic activity. Fundamentals and some future challenges are also described.

Elasmobranch qPCR reference genes: a case study of hypoxia preconditioned epaulette sharks

Directory of Open Access Journals (Sweden)

Ashton Kevin J

2010-04-01

Full Text Available Abstract Background Elasmobranch fishes are an ancient group of vertebrates which have high potential as model species for research into evolutionary physiology and genomics. However, no comparative studies have established suitable reference genes for quantitative PCR (qPCR in elasmobranchs for any physiological conditions. Oxygen availability has been a major force shaping the physiological evolution of vertebrates, especially fishes. Here we examined the suitability of 9 reference candidates from various functional categories after a single hypoxic insult or after hypoxia preconditioning in epaulette shark (Hemiscyllium ocellatum. Results Epaulette sharks were caught and exposed to hypoxia. Tissues were collected from 10 controls, 10 individuals with single hypoxic insult and 10 individuals with hypoxia preconditioning (8 hypoxic insults, 12 hours apart. We produced sequence information for reference gene candidates and monitored mRNA expression levels in four tissues: cerebellum, heart, gill and eye. The stability of the genes was examined with analysis of variance, geNorm and NormFinder. The best ranking genes in our study were eukaryotic translation elongation factor 1 beta (eef1b, ubiquitin (ubq and polymerase (RNA II (DNA directed polypeptide F (polr2f. The performance of the ribosomal protein L6 (rpl6 was tissue-dependent. Notably, in one tissue the analysis of variance indicated statistically significant differences between treatments for genes that were ranked as the most stable candidates by reference gene software. Conclusions Our results indicate that eef1b and ubq are generally the most suitable reference genes for the conditions and tissues in the present epaulette shark studies. These genes could also be potential reference gene candidates for other physiological studies examining stress in elasmobranchs. The results emphasise the importance of inter-group variation in reference gene evaluation.

Overview on CO{sub 2} Valorization: Challenge of Molten Carbonates

Energy Technology Data Exchange (ETDEWEB)

Chery, Déborah; Lair, Virginie; Cassir, Michel, E-mail: michel.cassir@chimie-paristech.fr [Chimie ParisTech, CNRS, Institut de Recherche de Chimie Paris, PSL Research University, Paris (France)

2015-10-02

The capture and utilization of CO{sub 2} is becoming progressively one of the significant challenges in the field of energetic resources. Whatever the energetic device, it is impossible to avoid completely the production of greenhouse gas, even parting from renewable energies. Transforming CO{sub 2} into a valuable fuel, such as alcohols, CO, or even C, could constitute a conceptual revolution in the energetic bouquet offering a huge application domain. Although several routes have been tested for this purpose, on which a general panorama will be given here, molten carbonates are attracting a renewed interest aiming at dissolving and reducing carbon dioxide in such melts. Because of their unique properties, molten carbonates are already used as electrolytes in molten carbonate fuel cells; they can also provoke a breakthrough in a new economy considering CO{sub 2} as an energetic source rather than a waste. Molten carbonates’ science and technology is becoming a strategic field of research for energy and environmental issues. Our aim in this review is to put in evidence the benefits of molten carbonates to valorize CO{sub 2} and to show that it is one of the most interesting routes for such application.

Challenges and Strategies for Proteome Analysis of the Interaction of Human Pathogenic Fungi with Host Immune Cells.

Science.gov (United States)

Krüger, Thomas; Luo, Ting; Schmidt, Hella; Shopova, Iordana; Kniemeyer, Olaf

2015-12-14

Opportunistic human pathogenic fungi including the saprotrophic mold Aspergillus fumigatus and the human commensal Candida albicans can cause severe fungal infections in immunocompromised or critically ill patients. The first line of defense against opportunistic fungal pathogens is the innate immune system. Phagocytes such as macrophages, neutrophils and dendritic cells are an important pillar of the innate immune response and have evolved versatile defense strategies against microbial pathogens. On the other hand, human-pathogenic fungi have sophisticated virulence strategies to counteract the innate immune defense. In this context, proteomic approaches can provide deeper insights into the molecular mechanisms of the interaction of host immune cells with fungal pathogens. This is crucial for the identification of both diagnostic biomarkers for fungal infections and therapeutic targets. Studying host-fungal interactions at the protein level is a challenging endeavor, yet there are few studies that have been undertaken. This review draws attention to proteomic techniques and their application to fungal pathogens and to challenges, difficulties, and limitations that may arise in the course of simultaneous dual proteome analysis of host immune cells interacting with diverse morphotypes of fungal pathogens. On this basis, we discuss strategies to overcome these multifaceted experimental and analytical challenges including the viability of immune cells during co-cultivation, the increased and heterogeneous protein complexity of the host proteome dynamically interacting with the fungal proteome, and the demands on normalization strategies in terms of relative quantitative proteome analysis.

Big data in complex systems challenges and opportunities

CERN Document Server

Azar, Ahmad; Snasael, Vaclav; Kacprzyk, Janusz; Abawajy, Jemal

2015-01-01

This volume provides challenges and Opportunities with updated, in-depth material on the application of Big data to complex systems in order to find solutions for the challenges and problems facing big data sets applications. Much data today is not natively in structured format; for example, tweets and blogs are weakly structured pieces of text, while images and video are structured for storage and display, but not for semantic content and search. Therefore transforming such content into a structured format for later analysis is a major challenge. Data analysis, organization, retrieval, and modeling are other  foundational challenges treated in this book. The material of this book will be useful for researchers and practitioners in the field of big data as well as advanced undergraduate and graduate  students. Each of the 17 chapters in the book opens with a chapter abstract and key terms list. The chapters are organized along the lines of problem description, related works, and analysis of the results and ...

D3R Grand Challenge 2: blind prediction of protein-ligand poses, affinity rankings, and relative binding free energies

Science.gov (United States)

Gaieb, Zied; Liu, Shuai; Gathiaka, Symon; Chiu, Michael; Yang, Huanwang; Shao, Chenghua; Feher, Victoria A.; Walters, W. Patrick; Kuhn, Bernd; Rudolph, Markus G.; Burley, Stephen K.; Gilson, Michael K.; Amaro, Rommie E.

2018-01-01

The Drug Design Data Resource (D3R) ran Grand Challenge 2 (GC2) from September 2016 through February 2017. This challenge was based on a dataset of structures and affinities for the nuclear receptor farnesoid X receptor (FXR), contributed by F. Hoffmann-La Roche. The dataset contained 102 IC50 values, spanning six orders of magnitude, and 36 high-resolution co-crystal structures with representatives of four major ligand classes. Strong global participation was evident, with 49 participants submitting 262 prediction submission packages in total. Procedurally, GC2 mimicked Grand Challenge 2015 (GC2015), with a Stage 1 subchallenge testing ligand pose prediction methods and ranking and scoring methods, and a Stage 2 subchallenge testing only ligand ranking and scoring methods after the release of all blinded co-crystal structures. Two smaller curated sets of 18 and 15 ligands were developed to test alchemical free energy methods. This overview summarizes all aspects of GC2, including the dataset details, challenge procedures, and participant results. We also consider implications for progress in the field, while highlighting methodological areas that merit continued development. Similar to GC2015, the outcome of GC2 underscores the pressing need for methods development in pose prediction, particularly for ligand scaffolds not currently represented in the Protein Data Bank (http://www.pdb.org), and in affinity ranking and scoring of bound ligands.

The economic effect and outcome of delaying oral food challenges.

Science.gov (United States)

Couch, Christopher; Franxman, Tim; Greenhawt, Matthew

2016-05-01

Food specific IgE (sIgE) is a useful marker to assess predictability of oral food challenge (OFC) outcome. A threshold of less than 2 kUA/L for peanut, egg, and milk has been proposed as a 50% negative predictive value at which patients may pass an OFC. To assess the economic effect and outcome of delaying OFCs. A retrospective analysis was performed for peanut, egg, and milk OFCs conducted between 2001 and 2012 at a tertiary food allergy referral center. Delayed OFC was defined as greater than 12 months from the time the sIgE level became less than 2 kUA/L. Time to OFC was explored in association with skin prick test result (wheal size), OFC outcome, and the economic effect of delay. Of 319 challenges, 173 OFCs were delayed (54.2%) by a mean time of 35.5 months (range, 13-123 months) vs a mean time of 4.2 months in the 146 challenges that were not delayed (P care system. Copyright © 2016 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

DREAMTools: a Python package for scoring collaborative challenges [version 2; referees: 1 approved, 2 approved with reservations

Directory of Open Access Journals (Sweden)

Thomas Cokelaer

2016-04-01

Full Text Available DREAM challenges are community competitions designed to advance computational methods and address fundamental questions in system biology and translational medicine. Each challenge asks participants to develop and apply computational methods to either predict unobserved outcomes or to identify unknown model parameters given a set of training data. Computational methods are evaluated using an automated scoring metric, scores are posted to a public leaderboard, and methods are published to facilitate community discussions on how to build improved methods. By engaging participants from a wide range of science and engineering backgrounds, DREAM challenges can comparatively evaluate a wide range of statistical, machine learning, and biophysical methods. Here, we describe DREAMTools, a Python package for evaluating DREAM challenge scoring metrics. DREAMTools provides a command line interface that enables researchers to test new methods on past challenges, as well as a framework for scoring new challenges. As of March 2016, DREAMTools includes more than 80% of completed DREAM challenges. DREAMTools complements the data, metadata, and software tools available at the DREAM website http://dreamchallenges.org and on the Synapse platform at https://www.synapse.org. Availability: DREAMTools is a Python package. Releases and documentation are available at http://pypi.python.org/pypi/dreamtools. The source code is available at http://github.com/dreamtools/dreamtools.

Anticholinesterase activity and chemical profile of an active chromatographic fraction of ethanolic extract from Bellis perennis L. (Asteraceae) flowers; Atividade anticolinesterasica e perfil quimico de uma fracao cromatografica ativa do extrato etanolico das flores Bellis perennis L. (Asteraceae)

Energy Technology Data Exchange (ETDEWEB)

Marques, Thiago Henrique Costa; Santos, Pauline Sousa dos; Freitas, Rivelilson Mendes de, E-mail: rivelilson@pq.cnpq.br [Universidade Federal do Piaui (UFPI), Teresina, PI (Brazil). Centro de Ciencias da Saude. Departamento de Bioquimica e Farmacologia; Carvalho, Rusbene Bruno Fonseca de; Melo, Cassio Herbert Santos de [Universidade Federal do Piaui (UFPI), Teresina, PI (Brazil). Centro de Ciencias da Natureza. Departamento de Quimica; David, Juceni Pereira [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Faculdade de Farmacia; David, Jorge Mauricio; Lima, Luciano Silva [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Instituto de Quimica

2013-09-01

This work describes the isolation of an active flavonoid fraction and identification of isorhamnetin 3-O-{beta}-D-(6''-acetyl)- alactopyranoside from flowers of B. perennis, and also the evaluation of anticholinesterase (AChE) activity of ethanolic extract from flowers (EEF) and the active fraction. The chemical structure of the flavonoid was defined on the basis of spectroscopic {sup 1}H NMR, IR and UV data. EEF or flavonoid reduces AChE activity in vivo, while flavonoid also reduces AChE activity in vitro, showing a value of 1.49 {mu}M for 50% inhibitory concentration (IC{sub 50}), suggesting potential use as an insecticide or in the treatment of neurodegenerative diseases such as Alzheimer's disease. (author)

AREVA solutions to licensing challenges in PWR and BWR reload and safety analysis

Energy Technology Data Exchange (ETDEWEB)

Curca-Tivig, Florin [AREVA GmbH, Erlangen (Germany)

2016-05-15

Regulatory requirements for reload and safety analyses are evolving: new safety criteria, request for enlarged qualification databases, statistical applications, uncertainty propagation.. In order to address these challenges and access more predictable licensing processes, AVERA is implementing consistent code and methodology suites for PWR and BWR core design and safety analysis, based on first principles modeling and extremely broad verification and validation data base. Thanks to the high computational power increase in the last decades methods' development and application now include new capabilities. An overview of the main AREVA codes and methods developments is given covering PWR and BWR applications in different licensing environments.

5-2-1-0 Activity and Nutrition Challenge for Elementary Students: New, Evidence-Based, Promising

Science.gov (United States)

Lovell, Cynthia Miller

2018-01-01

Obesity prevention in youth is a health priority, and teaching healthy habits toward this end is one of a school nurse's many responsibilities. A school nurse developed and implemented a school-wide, 2-week-long Activity and Nutrition Challenge (ANC) using the evidence-based 5-2-1-0 initiative to prevent and fight childhood obesity. Despite…

New LHCb Management readies for run 2 challenges

CERN Multimedia

Antonella Del Rosso

2014-01-01

As of 1 July, LHCb, one of the four biggest experiments at the LHC, will have a new Management. Ahead are the huge challenges of run 2 and the following long technical shutdown during which LHCb will undergo a major upgrade. In the meantime, the discovery of new physics could be a dream within reach…   New LHCb Spokesperson, Guy Wilkinson.   “We have to make sure that the detector wakes up after its long hibernation and goes back to data taking in the most efficient way and that we are able to process all these data to produce high-quality physics results,” says Guy Wilkinson, new Spokesperson of the LHCb collaboration. Although this already sounds like a considerable “to-do” list for the coming months, it’s just the beginning of a much longer and ambitious plan. “The previous management has done an excellent job in analysing the data we took during run 1. They also put on a very sound footing the LHCb upgrade, whi...

Experiences of Academic Members About their Professional Challenges: a Content Analysis Qualitative study.

Science.gov (United States)

Shahhosseini, Zohreh; Danesh, Mahmonier

2014-04-01

University faculty members of different disciplines in any country, by giving better quality services, will further accelerate the development of their respective countries. This study aims to explore the experiences of faculty members about their professional challenges. In this qualitative study, which was conducted in 2013, fifteen faculty members in the departments of clinical and basic sciences of Mazandaran university of Medical Sciences in northern Iran were chosen for semi-structured in-depth interviews by purposive sampling method. All tape-recorded data were fully transcribed and content analysis was performed. AFTER IMMERSION AND DATA ANALYSIS, THREE MAIN THEMES WERE EMERGED INCLUDING: "Imbalances in academic members' tasks in different areas", "Weakness of evaluation and promotion system" and "Failure to provide the infrastructure educational facilities". The main themes and sub-themes are explained by the help of participants' direct quotations. This study suggested that it is better to take effective measures to improve the faculty members' situation and therefore increase their efficiency, effectiveness and productivity.

Challenges in Mentoring Software Development Projects in the High School: Analysis According to Shulman's Teacher Knowledge Base Model

Science.gov (United States)

Meerbaum-Salant, Orni; Hazzan, Orit

2009-01-01

This paper focuses on challenges in mentoring software development projects in the high school and analyzes difficulties encountered by Computer Science teachers in the mentoring process according to Shulman's Teacher Knowledge Base Model. The main difficulties that emerged from the data analysis belong to the following knowledge sources of…

First Aid Challenge

Science.gov (United States)

Roman, Harry T.

2011-01-01

This article describes a challenge wherein students will be asked to design a portable first aid kit that is normally carried in a recreational vehicle (RV), but can also be hand-carried or backpacked off road for distances of approximately 1-2 miles. This can be a very practical challenge for the students because it touches everyone. Everybody…

Image analysis and machine learning in digital pathology: Challenges and opportunities.

Science.gov (United States)

Madabhushi, Anant; Lee, George

2016-10-01

With the rise in whole slide scanner technology, large numbers of tissue slides are being scanned and represented and archived digitally. While digital pathology has substantial implications for telepathology, second opinions, and education there are also huge research opportunities in image computing with this new source of "big data". It is well known that there is fundamental prognostic data embedded in pathology images. The ability to mine "sub-visual" image features from digital pathology slide images, features that may not be visually discernible by a pathologist, offers the opportunity for better quantitative modeling of disease appearance and hence possibly improved prediction of disease aggressiveness and patient outcome. However the compelling opportunities in precision medicine offered by big digital pathology data come with their own set of computational challenges. Image analysis and computer assisted detection and diagnosis tools previously developed in the context of radiographic images are woefully inadequate to deal with the data density in high resolution digitized whole slide images. Additionally there has been recent substantial interest in combining and fusing radiologic imaging and proteomics and genomics based measurements with features extracted from digital pathology images for better prognostic prediction of disease aggressiveness and patient outcome. Again there is a paucity of powerful tools for combining disease specific features that manifest across multiple different length scales. The purpose of this review is to discuss developments in computational image analysis tools for predictive modeling of digital pathology images from a detection, segmentation, feature extraction, and tissue classification perspective. We discuss the emergence of new handcrafted feature approaches for improved predictive modeling of tissue appearance and also review the emergence of deep learning schemes for both object detection and tissue classification

The Role of Protein Elongation Factor eEF1A2 in Breast Cancer

Science.gov (United States)

2006-09-01

apoptosis . A summary of common EMT markers is listed in Table I. Importantly, these developmental regulators can induce EMT in a nondevelopmental...1978; Chen et al., 1997), apoptosis (Chen et al., 1997), and metabolic regulation (Bissell et al., 1977) has been known for decades, the molecular...marks the Spemann organizer in vertebrate gastrulation and is one of the fi rst identifi ed regulators of embryological patterning (De Robertis et al

You pray to your God: A qualitative analysis of challenges in the provision of safe, timely, and affordable surgical care in Uganda.

Science.gov (United States)

Albutt, Katherine; Yorlets, Rachel R; Punchak, Maria; Kayima, Peter; Namanya, Didacus B; Anderson, Geoffrey A; Shrime, Mark G

2018-01-01

Five billion people lack access to safe, affordable, and timely surgical and anesthesia care. Significant challenges remain in the provision of surgical care in low-resource settings. Uganda is no exception. From September to November 2016, we conducted a mixed-methods countrywide surgical capacity assessment at 17 randomly selected public hospitals in Uganda. Researchers conducted 35 semi-structured interviews with key stakeholders to understand factors related to the provision of surgical care. The framework approach was used for thematic and explanatory data analysis. The Ugandan public health care sector continues to face significant challenges in the provision of safe, timely, and affordable surgical care. These challenges can be broadly grouped into preparedness and policy, service delivery, and the financial burden of surgical care. Hospital staff reported challenges including: (1) significant delays in accessing surgical care, compounded by a malfunctioning referral system; (2) critical workforce shortages; (3) operative capacity that is limited by inadequate infrastructure and overwhelmed by emergency and obstetric volume; (4) supply chain difficulties pertaining to provision of essential medications, equipment, supplies, and blood; (5) significant, variable, and sometimes catastrophic expenditures for surgical patients and their families; and (6) a lack of surgery-specific policies and priorities. Despite these challenges, innovative strategies are being used in the public to provide surgical care to those most in need. Barriers to the provision of surgical care are cross-cutting and involve constraints in infrastructure, service delivery, workforce, and financing. Understanding current strengths and shortfalls of Uganda's surgical system is a critical first step in developing effective, targeted policy and programming that will build and strengthen its surgical capacity.

Designing the robot inclusive space challenge

Directory of Open Access Journals (Sweden)

Rajesh Elara Mohan

2015-11-01

Full Text Available A novel robotic challenge, namely the robot inclusive spaces (RIS challenge, is proposed in this paper, which is a cross disciplinary and design focused initiative. It aims to foster the roboticists, architects, and designers towards realizing robot friendly social spaces. Contrary to conventional robotics competitions focusing on designing robots and its component technologies, robot inclusive spaces challenge adopts an interdisciplinary “design for robots” strategy to overcome the traditional research problem in real world deployments of social robots. In order to realize the RIS, various architectural elements must be adapted including: design principles for inclusive spaces, lighting schemes, furniture choices and arrangement, wall and floor surfaces, pathways among others. This paper introduces the format and design principles of RIS challenge, presents a first run of the challenge, and gives the corresponding analysis.

Confirmatory factor analysis of the Competitive State Anxiety Inventory-2.

Science.gov (United States)

Lane, A M; Sewell, D F; Terry, P C; Bartram, D; Nesti, M S

1999-06-01

The aim of this study was to evaluate the factor structure of the Competitive State Anxiety Inventory-2 (CSAI-2) using confirmatory factor analysis. Volunteer participants (n = 1213) completed the CSAI-2 approximately 1 h before competition and the data were analysed in two samples. The hypothesized model showed poor fit indices in both samples independently (Robust Comparative Fit Index: sample A = 0.82, sample B = 0.84) and simultaneously (Comparative Fit Index = 0.83), suggesting that the factor structure proposed by Martens et al. is flawed. Our findings suggest that a limitation of the Cognitive Anxiety scale derives from phrasing items around the word 'concerned' rather than 'worried'. We suggest that being concerned about an impending performance does not necessarily mean that an athlete is experiencing negative thoughts, but that the athlete is acknowledging the importance and difficulty of the challenge and is attempting to mobilize resources to cope. The present results question the use of the CSAI-2 as a valid measure of competitive state anxiety.

Clinical comparison of overlapping deletions of 19p13.3.

Science.gov (United States)

Risheg, Hiba; Pasion, Romela; Sacharow, Stephanie; Proud, Virginia; Immken, LaDonna; Schwartz, Stuart; Tepperberg, Jim H; Papenhausen, Peter; Tan, Tiong Y; Andrieux, Joris; Plessis, Ghislaine; Amor, David J; Keitges, Elisabeth A

2013-05-01

We present three patients with overlapping interstitial deletions of 19p13.3 identified by high resolution SNP microarray analysis. All three had a similar phenotype characterized by intellectual disability or developmental delay, structural heart abnormalities, large head relative to height and weight or macrocephaly, and minor facial anomalies. Deletion sizes ranged from 792 Kb to 1.0 Mb and included a common region arr [hg19] 19p13.3 (3,814,392-4,136,989), containing eight genes: ZFR2, ATCAY, NMRK2, DAPK3, EEF2, PIAS4, ZBTB7A, MAP2K2, and two non-coding RNA's MIR637 and SNORDU37. The patient phenotypes were compared with three previous single patient reports with similar interstitial 19p13.3 deletions and six additional patients from the DECIPHER and ISCA databases to determine if a common haploinsufficient phenotype for the region can be established. Copyright © 2013 Wiley Periodicals, Inc.

Lessons we most enjoy learning.

Science.gov (United States)

Coleman, P L

1993-03-01

In the mid-1970s, the Philippine Commission on Population (POPCOM) began to use entertainment programs for reaching people with messages on population and development issues. 2 major motion pictures contained family planning (FP) messages. Radio dramas, print media, and theater also were used to convey FP messages. The early experiments were continued in the late 1980s through the work of the Philippine Center for Population and Development (PCPD). PCPD, with the assistance of the Johns Hopkins University/Population Communication Services (JHU/PCS) project, embarked on a program which used popular music to encourage young people to become sexually responsible adults. In 1990, the Philippine Non-Governmental Organization Council (PNGOC), the Department of Health (DOH) and JHU/PCS began an effort funded by USAID to form a coalition with the entertainment community for social development causes. DOH, JHU/PCS, and USAID wanted to promote FP and health through the Enter-Educate concept. PNGOC and JHU/PCS contacted over 20 entertainment organizations and held more than 75 conferences, work shops, and meetings which attended by more than 300 people. The movement of Entertainment for Social Change was launched in October 1991 with the creation of the Enter-Educate Foundation, Inc. (EEF). The aims of EEF include rewards, professional approach, and establishment of a network of dedicated entertainment and social development professionals. In 1993, a television comedy series will focus on FP as well as on maternal and child health. Further plans at the local level include: tree planting; discussions on migration; talks about FP; meetings on community population and environment activities; and networking of organizations involved with youth, the environment, and population. JHU/PCS provides technical assistance for the production, monitoring, and evaluation of the project. With these efforts, the EEF is attempting to focus on the country's biggest problems: population and the

Paediatric oral peanut challenges: a comparison of practice in London and western Switzerland.

Science.gov (United States)

Ludman, S; Wassenberg, J; Du Toit, G; Fox, A T; Lack, G; Eigenmann, P A

2013-04-01

There are guidelines on how to develop a food challenge protocol, but at present there is no gold standard guidance on method, and separate units produce differing protocols. We performed a retrospective analysis of 200 patients' data from the paediatric allergy units in Lausanne and Geneva, Western Switzerland, and St Thomas' Hospital (STH), UK. St Thomas' Hospital has a younger cohort with a lower overall mean spIgE (2.36 kU/l vs. 8.00 kU/l, P = 0.004). The target peanut protein volumes differed: Switzerland 4.4 g vs. STH 8.4 g. Despite this, the dose actually achieved in positive challenges was not significantly different (2.33 g vs. 1.49 g, P = 0.16). 26% of challenges reacted at 4 g or more of peanut protein. The differences in results highlight how the variation in reasoning behind food challenge alters the outcome. Standardization of food challenges would allow easy comparison between hospitals and geographical areas for research purposes. © 2013 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd.

Data management challenges in analysis and synthesis in the ecosystem sciences.

Science.gov (United States)

Specht, A; Guru, S; Houghton, L; Keniger, L; Driver, P; Ritchie, E G; Lai, K; Treloar, A

2015-11-15

Open-data has created an unprecedented opportunity with new challenges for ecosystem scientists. Skills in data management are essential to acquire, manage, publish, access and re-use data. These skills span many disciplines and require trans-disciplinary collaboration. Science synthesis centres support analysis and synthesis through collaborative 'Working Groups' where domain specialists work together to synthesise existing information to provide insight into critical problems. The Australian Centre for Ecological Analysis and Synthesis (ACEAS) served a wide range of stakeholders, from scientists to policy-makers to managers. This paper investigates the level of sophistication in data management in the ecosystem science community through the lens of the ACEAS experience, and identifies the important factors required to enable us to benefit from this new data-world and produce innovative science. ACEAS promoted the analysis and synthesis of data to solve transdisciplinary questions, and promoted the publication of the synthesised data. To do so, it provided support in many of the key skillsets required. Analysis and synthesis in multi-disciplinary and multi-organisational teams, and publishing data were new for most. Data were difficult to discover and access, and to make ready for analysis, largely due to lack of metadata. Data use and publication were hampered by concerns about data ownership and a desire for data citation. A web portal was created to visualise geospatial datasets to maximise data interpretation. By the end of the experience there was a significant increase in appreciation of the importance of a Data Management Plan. It is extremely doubtful that the work would have occurred or data delivered without the support of the Synthesis centre, as few of the participants had the necessary networks or skills. It is argued that participation in the Centre provided an important learning opportunity, and has resulted in improved knowledge and understanding

BASE - 2nd generation software for microarray data management and analysis

Directory of Open Access Journals (Sweden)

Nordborg Nicklas

2009-10-01

Full Text Available Abstract Background Microarray experiments are increasing in size and samples are collected asynchronously over long time. Available data are re-analysed as more samples are hybridized. Systematic use of collected data requires tracking of biomaterials, array information, raw data, and assembly of annotations. To meet the information tracking and data analysis challenges in microarray experiments we reimplemented and improved BASE version 1.2. Results The new BASE presented in this report is a comprehensive annotable local microarray data repository and analysis application providing researchers with an efficient information management and analysis tool. The information management system tracks all material from biosource, via sample and through extraction and labelling to raw data and analysis. All items in BASE can be annotated and the annotations can be used as experimental factors in downstream analysis. BASE stores all microarray experiment related data regardless if analysis tools for specific techniques or data formats are readily available. The BASE team is committed to continue improving and extending BASE to make it usable for even more experimental setups and techniques, and we encourage other groups to target their specific needs leveraging on the infrastructure provided by BASE. Conclusion BASE is a comprehensive management application for information, data, and analysis of microarray experiments, available as free open source software at http://base.thep.lu.se under the terms of the GPLv3 license.

BASE--2nd generation software for microarray data management and analysis.

Science.gov (United States)

Vallon-Christersson, Johan; Nordborg, Nicklas; Svensson, Martin; Häkkinen, Jari

2009-10-12

Microarray experiments are increasing in size and samples are collected asynchronously over long time. Available data are re-analysed as more samples are hybridized. Systematic use of collected data requires tracking of biomaterials, array information, raw data, and assembly of annotations. To meet the information tracking and data analysis challenges in microarray experiments we reimplemented and improved BASE version 1.2. The new BASE presented in this report is a comprehensive annotable local microarray data repository and analysis application providing researchers with an efficient information management and analysis tool. The information management system tracks all material from biosource, via sample and through extraction and labelling to raw data and analysis. All items in BASE can be annotated and the annotations can be used as experimental factors in downstream analysis. BASE stores all microarray experiment related data regardless if analysis tools for specific techniques or data formats are readily available. The BASE team is committed to continue improving and extending BASE to make it usable for even more experimental setups and techniques, and we encourage other groups to target their specific needs leveraging on the infrastructure provided by BASE. BASE is a comprehensive management application for information, data, and analysis of microarray experiments, available as free open source software at http://base.thep.lu.se under the terms of the GPLv3 license.

Expression of the Y-Encoded TSPY is Associated with Progression of Prostate Cancer

Directory of Open Access Journals (Sweden)

Tatsuo Kido

2010-09-01

Full Text Available TSPY is a Y-encoded gene that is expressed in normal testicular germ cells and various cancer types including germ cell tumor, melanoma, hepatocellular carcinoma, and prostate cancer. Currently, the correlation between TSPY expression and oncogenic development has not been established, particularly in somatic cancers. To establish such correlation, we analyzed the expression of TSPY, in reference to its interactive oncoprotein, EEF1A, tumor biomarker, AMACR, and normal basal cell biomarker, p63, in 41 cases of clinical prostate cancers (CPCa, 17 cases of latent prostate cancers (LPCa, and 19 cases of non-cancerous prostate (control by immunohistochemistry. Our results show that TSPY was detected more frequently (78% in the clinical prostate cancer specimens than those of latent prostate cancer (47% and control (50%. In the latent cancer group, the levels of TSPY expression could be correlated with increasing Gleason grades. TSPY expression was detected in seven out of nine high-grade latent cancer samples (Gleason 7 and more. The expression of the TSPY binding partner EEF1A was detectable in all prostate specimens, but the levels were higher in cancer cells in clinical and latent prostate cancer specimens than normal prostatic cells. These observations suggest that expressions of TSPY and its binding partner EEF1A are associated with the development and progression of prostate cancer.

Meeting new challenges in radon measurements service with solid state alpha track analysis

International Nuclear Information System (INIS)

Calamosca, M.; Penzo, S.; Mustapha, A.O.

2005-01-01

Full text: The national and international legislation on the control of exposures to radiation now covers exposures to the naturally occurring radioactive materials (NORM), with particular emphasis on radon in workplaces. Consequently, many more working environments have now been brought to the realm of radiation monitoring. This is mirrored by a corresponding increase in the demands for radon monitoring service. The new challenges occasioned by the increase in demands are illustrated in this paper. The paper also describes the new integrated radon measurement system at the ENEA ION-IRP radon-in-air testing laboratory, designed to meet the new challenges. In this presentation emphasis is laid only on the special features, hardware and software, of the measurement system that directly result in enhancement of the overall throughput and the quality of the radon service. In addition the use of a new technique based on a numerical filtering of the main physical parameters of the alpha track analysis used to determine the radon exposure with a CR-39 detector is presented, with particular emphasis to the quality improvements of the measurement. The readout procedure adopted by the Enea radon Service is based on track analysis by light microscopy. Since the dimensions of the tracks are significantly affected by the light intensity, the illumination of the field of view (FOV) has to be as homogeneous as possible, in order to avoid sensitivity dependence on track position in the FOV. The lighting variation could be severe, especially at the borders or when a rectangular FOV is used. The coefficient of variation of the FOV light uniformity has to be periodically checked to maintain the variation below an acceptable limit, whereas if the results exceed this value a remedial action is mandatory. The numerical correction procedure here presented results effective. (author)

Mystery photos: challenge No. 2!

CERN Multimedia

Alex Brown, Jens Vigen

2014-01-01

In the last issue of the Bulletin, we launched a public appeal for information to identify some CERN pictures that have recently been digitised by the CERN Library. The response has been huge. The story was picked up on many websites and discussion forums all over the Web, and hundreds of people sent in their suggestions. Time for a new challenge.   Many thousands of CERN's archived pictures are currently being scanned and uploaded to the CERN Document Server. While most of these pictures can be matched to existing historical information, many are harder to identify. Following the recent article in the last issue of the Bulletin, we have received submissions in five different languages from a dozen countries. We would like to express our thanks to everyone who has contributed their answers. We have now identified all of the pictures highlighted in the last issue of the Bulletin and we are updating the records accordingly. You can see them all here. To see a complete lis...

Genomic Research Data Generation, Analysis and Sharing – Challenges in the African Setting

Directory of Open Access Journals (Sweden)

Nicola Mulder

2017-11-01

and expensive computing infrastructure which are often unavailable. Recently initiatives such as H3Africa and H3ABioNet which aim to build capacity for large-scale genomics projects in Africa have emerged. Here we describe such initiatives, including the challenges faced in the generation, analysis and sharing of genomic data and how these challenges are being overcome.

[Immunogenicity and protective efficacy of pertactin recombinants against Bordetella bronchiseptica challenge].

Science.gov (United States)

Zhao, Zhanqin; Wang, Chen; Xue, Yun; Ding, Ke; Zhang, Chunjie; Cheng, Xiangchao; Li, Yinju; Liu, Yichen; Wu, Tingcai

2010-09-01

In this study we showed the immunogenicity and protective efficacy of five pertactin recombinants against Bordetella bronchiseptica (Bb) challenge. The complete coding sequence (2040 bp) of the prn gene (PRN) and its fragments,5'-terminal 1173 bp fragment (PN),3'-terminal 867 bp fragment (PC), two copies of region I (654 bp; PR I) in PN, and 2 copies of region II (678 bp; PR II) in PC, were separately cloned into the prokaryotic expression vector pGEX-KG, and expressed in the Eschierichia coli BL21 (DE3) using induction by isopropyl-beta-D-thiogalactopyranoside. The recombinant proteins were named GST-PRN, GST-PN, GST-PC, GST-2PR I and GST-2PR II. All five recombinant proteins showed immunological reactivity in the Western-blot analysis. Mice, immunized subcutaneously with two doses of the purified proteins mixed with an equal volume of Freund's adjuvant,produced robust PRN-specific IgG antibody levels. When challenged, 6 of 9 mice in GST-2PR I group and all 9 mice in the other groups survived intranasal challenge with three times the 50% lethal dose (LD50) of virulent Bb HH0809. After challenge with 10 LD50 7/9,3/9,6/9,1/10 and 6/10 of the mice survived. Furthermore, complete protection against intraperitoneal (i.p.) challenge with 10 LD50 of HH0809 was observed in mice that were injected i.p. with 0.5 ml rabbit anti-GST-PRN, GST-PN,GST-PC or GST-2PR II serum. Only 1 of 10 mice survived in the group of mice that received anti-GST-2PR I, and no survivors were noted in the group of mice that received PRN-absorbed rabbit antiserum (0/5). In this study,we showed that all of five pertactin recombinants had differential immunogenicity and protective efficacy against Bb challenge. Mice immunized with GST-PC had better survival against fatal Bb challenge than did those immunized with GST-PN. In addition, GST-2PR II and GST-2PR I provided the similar results These data may have implications for the development of safe and efficacious subunit vaccines for the prevention of

Opportunities and Challenges for Technology Development and Adoption in Public Libraries

DEFF Research Database (Denmark)

Serholt, Sofia; Eriksson, Eva; Dalsgaard, Peter

2018-01-01

In this paper, we discuss opportunities and challenges for technology development and adoption in public libraries. The results are based on a multi-site comparative study and thematic analysis of six months of extensive ethnographic work in libraries in three different European countries....... The results explore the socio-technical practices, understandings, and perspectives of library staff and patrons when it comes to the role(s) and function(s) of libraries today. The contribution of this paper is two fold. Firstly, the results from the analysis of rich ethnographic data presented under six...... themes. Secondly, we offer a list of identified key opportunities and challenges focusing on 1) media and technology literacy, 2) institutional transformation and technical infrastructures, 3) resource constraints among library staff, and 4) a shift in focus towards supporting activities....

GenMAPP 2: new features and resources for pathway analysis

Directory of Open Access Journals (Sweden)

Dahlquist Kam D

2007-06-01

Full Text Available Abstract Background Microarray technologies have evolved rapidly, enabling biologists to quantify genome-wide levels of gene expression, alternative splicing, and sequence variations for a variety of species. Analyzing and displaying these data present a significant challenge. Pathway-based approaches for analyzing microarray data have proven useful for presenting data and for generating testable hypotheses. Results To address the growing needs of the microarray community we have released version 2 of Gene Map Annotator and Pathway Profiler (GenMAPP, a new GenMAPP database schema, and integrated resources for pathway analysis. We have redesigned the GenMAPP database to support multiple gene annotations and species as well as custom species database creation for a potentially unlimited number of species. We have expanded our pathway resources by utilizing homology information to translate pathway content between species and extending existing pathways with data derived from conserved protein interactions and coexpression. We have implemented a new mode of data visualization to support analysis of complex data, including time-course, single nucleotide polymorphism (SNP, and splicing. GenMAPP version 2 also offers innovative ways to display and share data by incorporating HTML export of analyses for entire sets of pathways as organized web pages. Conclusion GenMAPP version 2 provides a means to rapidly interrogate complex experimental data for pathway-level changes in a diverse range of organisms.

Challenges and Opportunities of Airborne Metagenomics

KAUST Repository

Behzad, H.

2015-05-06

Recent metagenomic studies of environments, such as marine and soil, have significantly enhanced our understanding of the diverse microbial communities living in these habitats and their essential roles in sustaining vast ecosystems. The increase in the number of publications related to soil and marine metagenomics is in sharp contrast to those of air, yet airborne microbes are thought to have significant impacts on many aspects of our lives from their potential roles in atmospheric events such as cloud formation, precipitation, and atmospheric chemistry to their major impact on human health. In this review, we will discuss the current progress in airborne metagenomics, with a special focus on exploring the challenges and opportunities of undertaking such studies. The main challenges of conducting metagenomic studies of airborne microbes are as follows: 1) Low density of microorganisms in the air, 2) efficient retrieval of microorganisms from the air, 3) variability in airborne microbial community composition, 4) the lack of standardized protocols and methodologies, and 5) DNA sequencing and bioinformatics-related challenges. Overcoming these challenges could provide the groundwork for comprehensive analysis of airborne microbes and their potential impact on the atmosphere, global climate, and our health. Metagenomic studies offer a unique opportunity to examine viral and bacterial diversity in the air and monitor their spread locally or across the globe, including threats from pathogenic microorganisms. Airborne metagenomic studies could also lead to discoveries of novel genes and metabolic pathways relevant to meteorological and industrial applications, environmental bioremediation, and biogeochemical cycles.

Do textbooks address known learning challenges in area measurement? A comparative analysis

Science.gov (United States)

Hong, Dae S.; Choi, Kyong Mi; Runnalls, Cristina; Hwang, Jihyun

2018-02-01

This study compared area lessons from Korean textbooks and US standard-based textbooks to understand differences and similarities among these textbooks, as well as how these textbooks address known learning challenges in area measurement. Several well-known challenges have been identified in previous studies, such as covering, array structure, and linking array structure to area formula. We were interested in knowing if textbooks addressed these issues in their treatments of area measurement and, in doing so, provided students with opportunities to overcome or become familiar with known challenges. The results show that both countries' textbooks demonstrated similar limitations; only few area and area-related lessons are covered and three important learning challenges in area measurement are not covered well, which need to be informed to practicing teachers.

Cervical mucus and serum estradiol as predictors of response to progestin challenge.

Science.gov (United States)

Rarick, L D; Shangold, M M; Ahmed, S W

1990-08-01

The present study was undertaken to assess the correlation between and relative predictive value of each of the following variables and progestin-induced withdrawal bleeding: cervical mucus appearance, serum E2 level, patient age, duration of amenorrhea, smoking and exercise habits, and body composition. Of 120 oligomenorrheic and amenorrheic women evaluated, only cervical mucus appearance and serum E2 level were significantly associated with response to progestin challenge. A multivariate logistical regression analysis showed cervical mucus to be the most predictive variable followed by serum E2 level. No absolute E2 level was found to discriminate between those who did and those who did not have withdrawal bleeding after progestin challenge. These data suggest that office examination of cervical mucus may be a useful indicator and guideline in planning therapy.

Control challenges in domestic heating systems

DEFF Research Database (Denmark)

Thybo, Honglian; Larsen, Lars F. S.; Weitzmann, Peter

2007-01-01

The objective of this paper is to analyze domestic heating applications and identify unfavorable building constructions and control challenges to be addressed by high performance heating control systems. Heating of domestic houses use a large amount of the total energy consumption in Scandinavia....... Hence the potential of reducing energy consumption by applying high performance control is vast. Indoor climate issues are becoming more in focus, which also leads to a demand for high performance heating systems. The paper presents an analysis of how the building elements of today's domestic houses...... with water based floor heating affect the control challenge. The analysis is documented with simulation results....

Challenges in reducing the computational time of QSTS simulations for distribution system analysis.

Energy Technology Data Exchange (ETDEWEB)

Deboever, Jeremiah [Georgia Inst. of Technology, Atlanta, GA (United States); Zhang, Xiaochen [Georgia Inst. of Technology, Atlanta, GA (United States); Reno, Matthew J. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Broderick, Robert Joseph [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Grijalva, Santiago [Georgia Inst. of Technology, Atlanta, GA (United States); Therrien, Francis [CME International T& D, St. Bruno, QC (Canada)

2017-06-01

The rapid increase in penetration of distributed energy resources on the electric power distribution system has created a need for more comprehensive interconnection modelling and impact analysis. Unlike conventional scenario - based studies , quasi - static time - series (QSTS) simulation s can realistically model time - dependent voltage controllers and the diversity of potential impacts that can occur at different times of year . However, to accurately model a distribution system with all its controllable devices, a yearlong simulation at 1 - second resolution is often required , which could take conventional computers a computational time of 10 to 120 hours when an actual unbalanced distribution feeder is modeled . This computational burden is a clear l imitation to the adoption of QSTS simulation s in interconnection studies and for determining optimal control solutions for utility operations . Our ongoing research to improve the speed of QSTS simulation has revealed many unique aspects of distribution system modelling and sequential power flow analysis that make fast QSTS a very difficult problem to solve. In this report , the most relevant challenges in reducing the computational time of QSTS simulations are presented: number of power flows to solve, circuit complexity, time dependence between time steps, multiple valid power flow solutions, controllable element interactions, and extensive accurate simulation analysis.

Challenges in quantifying biosphere-atmosphere exchange of nitrogen species

Energy Technology Data Exchange (ETDEWEB)

Sutton, M.A. [Centre for Ecology and Hydrology (CEH), Edinburgh Research Station, Bush Estate, Penicuik, EH26 0QB (United Kingdom)], E-mail: ms@ceh.ac.uk; Nemitz, E. [Centre for Ecology and Hydrology (CEH), Edinburgh Research Station, Bush Estate, Penicuik, EH26 0QB (United Kingdom); Erisman, J.W. [ECN, Clean Fossil Fuels, PO Box 1, 1755 ZG Petten (Netherlands); Beier, C. [Riso National Laboratory, PO Box 49, DK-4000 Roskilde (Denmark); Bahl, K. Butterbach [Institute of Meteorology and Climate Research, Atmos. Environ. Research (IMK-IFU), Research Centre Karlsruhe GmbH, Kreuzeckbahnstr. 19, 82467 Garmisch-Partenkirchen (Germany); Cellier, P. [INRA Unite Mixte de Recherche, 78850 Thiverval-Grignon (France); Vries, W. de [Alterra, Green World Research, PO Box 47, 6700 AA Wageningen (Netherlands); Cotrufo, F. [Dip. Scienze Ambientali, Seconda Universita degli Studi di Napoli, via Vivaldi 43, 81100 Caserta (Italy); Skiba, U.; Di Marco, C.; Jones, S. [Centre for Ecology and Hydrology (CEH), Edinburgh Research Station, Bush Estate, Penicuik, EH26 0QB (United Kingdom); Laville, P.; Soussana, J.F.; Loubet, B. [INRA Unite Mixte de Recherche, 78850 Thiverval-Grignon (France); Twigg, M.; Famulari, D. [Centre for Ecology and Hydrology (CEH), Edinburgh Research Station, Bush Estate, Penicuik, EH26 0QB (United Kingdom); Whitehead, J.; Gallagher, M.W. [School of Earth, Atmospheric and Environmental Sciences, University of Manchester, Williamson Building, Oxford Road, Manchester, M13 9PL (United Kingdom); Neftel, A.; Flechard, C.R. [Agroscope FAL Reckenholz, Federal Research Station for Agroecology and Agriculture, PO Box, CH 8046 Zurich (Switzerland)] (and others)

2007-11-15

Recent research in nitrogen exchange with the atmosphere has separated research communities according to N form. The integrated perspective needed to quantify the net effect of N on greenhouse-gas balance is being addressed by the NitroEurope Integrated Project (NEU). Recent advances have depended on improved methodologies, while ongoing challenges include gas-aerosol interactions, organic nitrogen and N{sub 2} fluxes. The NEU strategy applies a 3-tier Flux Network together with a Manipulation Network of global-change experiments, linked by common protocols to facilitate model application. Substantial progress has been made in modelling N fluxes, especially for N{sub 2}O, NO and bi-directional NH{sub 3} exchange. Landscape analysis represents an emerging challenge to address the spatial interactions between farms, fields, ecosystems, catchments and air dispersion/deposition. European up-scaling of N fluxes is highly uncertain and a key priority is for better data on agricultural practices. Finally, attention is needed to develop N flux verification procedures to assess compliance with international protocols. - Current N research is separated by form; the challenge is to link N components, scales and issues.

The NASA Langley Multidisciplinary Uncertainty Quantification Challenge

Science.gov (United States)

Crespo, Luis G.; Kenny, Sean P.; Giesy, Daniel P.

2014-01-01

This paper presents the formulation of an uncertainty quantification challenge problem consisting of five subproblems. These problems focus on key aspects of uncertainty characterization, sensitivity analysis, uncertainty propagation, extreme-case analysis, and robust design.

Experiencing work as a daily challenge: the case of scleroderma.

Science.gov (United States)

Mendelson, Cindy; Poole, Janet L; Allaire, Saralynn

2013-01-01

Little is known about the physical and discretionary aspects of work that people with scleroderma (SSc) find difficult. This article describes the findings from a study that explored the challenges and adaptations made by individuals with SSc to continue to work. Thirty-two employed individuals with SSc participated. Participants were predominantly women (82%), white (79%), and well educated (M = 16.9 years). The average age was 47.3 years, and 60.6% were married. Mean disease duration was 9.7 years, and 56.2% had diffuse SSc. Mean years on the job was 10.2 (SD ± 8.8), and 71.9% worked at least 35 hours per week. Participants engaged in a single structured interview about work-related challenges and adaptations. Content and thematic analysis was used to identify key themes across the interviews. Employees with SSc experienced Work as a daily challenge. This central theme described the general work experience for most participants. Three subthemes described their specific experiences: The work environment: Opportunities, challenges, and accommodations; Career planning; and Supportive others. The participants were anxious to find scenarios that allowed them to continue to work. Worksite accommodations and flexibility in scheduling can make the difference between working and disability.

Yueju Pill Rapidly Induces Antidepressant-Like Effects and Acutely Enhances BDNF Expression in Mouse Brain

Directory of Open Access Journals (Sweden)

Wenda Xue

2013-01-01

Full Text Available The traditional antidepressants have a major disadvantage in delayed onset of efficacy, and the emerging fast-acting antidepressant ketamine has adverse behavioral and neurotoxic effects. Yueju pill, an herb medicine formulated eight hundred years ago by Doctor Zhu Danxi, has been popularly prescribed in China for alleviation of depression-like symptoms. Although several clinical outcome studies reported the relative short onset of antidepressant effects of Yueju, this has not been scientifically investigated. We, therefore, examined the rapid antidepressant effect of Yueju in mice and tested the underlying molecular mechanisms. We found that acute administration of ethanol extract of Yueju rapidly attenuated depressive-like symptoms in learned helpless paradigm, and the antidepressant-like effects were sustained for at least 24 hours in tail suspension test in ICR mice. Additionally, Yueju, like ketamine, rapidly increased the expression of brain-derived neurotrophic factor (BDNF in the hippocampus, whereas the BDNF mRNA expression remained unaltered. Yueju rapidly reduced the phosphorylation of eukaryotic elongation factor 2 (eEF2, leading to desuppression of BDNF synthesis. Unlike ketamine, both the BDNF expression and eEF2 phosphorylation were revered at 24 hours after Yueju administration. This study is the first to demonstrate the rapid antidepressant effects of an herb medicine, offering an opportunity to improve therapy of depression.

Criticality analysis for mixed thorium-uranium fuel in the Angra-2 PWR reactor using KENO-VI

Energy Technology Data Exchange (ETDEWEB)

Wichrowski, Caio C.; Gonçalves, Isadora C.; Oliveira, Claudio L.; Vellozo, Sergio O.; Baptista, Camila O., E-mail: wichrowski@ime.eb.br, E-mail: isadora.goncalves@ime.eb.br, E-mail: d7luiz@yahoo.com.br, E-mail: vellozo@ime.eb.br, E-mail: camila.oliv.baptista@gmail.com [Instituto Militar de Engenharia (IME), Rio de Janeiro, RJ (Brazil). Seção de Engenharia Nuclear

2017-07-01

The increasing energy demand associated to the current sustainability challenges have given the thorium nuclear fuel cycle renewed interest in the scientific community. Studies have focused on energy production in different reactor designs through the fission of uranium 233, the product of thorium fertilization by neutrons. In order to make it possible for near future applications a strategy based on the adaptation of current nuclear reactors for the use of thorium fuels is being considered. In this work, bearing in mind these limitations, a code was used to evaluate the effect on criticality (k{sub inf}) of the mixing of thorium and uranium in different proportions in the fuel of a PWR, the German designed Angra-2 Brazilian reactor in order to scrutinise its behaviour and determine the feasibility of an adapted ThO{sub 2}-UO{sub 2} mixed fuel cycle using current PWR technology. The analysis is performed using the KENO-VI module in the SCALE 6.1 nuclear safety analysis simulation code and the information is taken from the Angra-2 FSAR (Final Security Analysis Report). (author)

New Challenges for Asset Managers in the Danish Social Housing Sector

DEFF Research Database (Denmark)

Haugbølle, Kim; Engberg, Lars A.

2004-01-01

During the 1990s, the social housing sector in Denmark has undergone a number of changes. New trends are forcing asset managers to rethink their roles and strategies. In this changing context, asset managers have been confronted with strategic challenges on three topics: 1. New modes of tenants......’ involvement. 2. New partnerships with the building industry. 3. Moving towards a European internal market of construction products. The paper explores and analyses these three challenges by drawing on a number of case studies as well as a re-analysis of current research on management of renewal and building...

Inhibition of eukaryotic translation elongation by the antitumor natural product Mycalamide B.

Science.gov (United States)

Dang, Yongjun; Schneider-Poetsch, Tilman; Eyler, Daniel E; Jewett, John C; Bhat, Shridhar; Rawal, Viresh H; Green, Rachel; Liu, Jun O

2011-08-01

Mycalamide B (MycB) is a marine sponge-derived natural product with potent antitumor activity. Although it has been shown to inhibit protein synthesis, the molecular mechanism of action by MycB remains incompletely understood. We verified the inhibition of translation elongation by in vitro HCV IRES dual luciferase assays, ribosome assembly, and in vivo [(35)S]methinione labeling experiments. Similar to cycloheximide (CHX), MycB inhibits translation elongation through blockade of eEF2-mediated translocation without affecting the eEF1A-mediated loading of tRNA onto the ribosome, AUG recognition, or dipeptide synthesis. Using chemical footprinting, we identified the MycB binding site proximal to the C3993 28S rRNA residue on the large ribosomal subunit. However, there are also subtle, but significant differences in the detailed mechanisms of action of MycB and CHX. First, MycB arrests the ribosome on the mRNA one codon ahead of CHX. Second, MycB specifically blocked tRNA binding to the E-site of the large ribosomal subunit. Moreover, they display different polysome profiles in vivo. Together, these observations shed new light on the mechanism of inhibition of translation elongation by MycB.

Strategic positioning. Part 2: Positioning challenges in an evolving health care marketplace.

Science.gov (United States)

Kauer, R T; Berkowitz, E

1997-01-01

Why is strategic positioning so important to health care organizations struggling in a managed care environment and what are the sources of value? In Part 1 of this article, entitled "The Sources of Value under Managed Care," the authors presented four sources of value relative to the evolution of the market from fee-for-service to managed care. These value sources are: (1) assets, (2) price/performance, (3) distribution, and, ultimately, (4) capabilities and brand equity. In this article, the authors further elaborate on the sources of value as the market moves beyond the historical fee-for-service position to a managed care marketplace. Part 2 presents the marketing and financial challenges to organizational positioning and performance across the four stages of managed care.

The Electric Vehicles Ecosystem Model: Construct, Analysis and Identification of Key Challenges

Directory of Open Access Journals (Sweden)

Zulkarnain

2014-09-01

Full Text Available This paper builds a conceptual model of electric vehicles’ (EV ecosystem and value chain build-up. Based on the literature, the research distinguishes the most critical challenges that are on the way of mobility systems’ electrification. Consumers still have some questions that call for answers before they are ready to adopt evs.With regard to technical aspects, some challenges are coming from vehicles, charging infrastructure, battery technology, and standardization. The use of battery in EVs will bring in additional environmental challenges, coming from the battery life cycle for used battery, the manufacturing, and from some materials used and treated in the manufacturing process. The policy aspects include mostly taxation strategies. For most part, established market conditions are still lacking and there are a number of unresolved challenges on both supply and demand side of the EV market.

Estimating challenge load due to disease outbreaks and other challenges using reproduction records of sows.

Science.gov (United States)

Mathur, P K; Herrero-Medrano, J M; Alexandri, P; Knol, E F; ten Napel, J; Rashidi, H; Mulder, H A

2014-12-01

A method was developed and tested to estimate challenge load due to disease outbreaks and other challenges in sows using reproduction records. The method was based on reproduction records from a farm with known disease outbreaks. It was assumed that the reduction in weekly reproductive output within a farm is proportional to the magnitude of the challenge. As the challenge increases beyond certain threshold, it is manifested as an outbreak. The reproduction records were divided into 3 datasets. The first dataset called the Training dataset consisted of 57,135 reproduction records from 10,901 sows from 1 farm in Canada with several outbreaks of porcine reproductive and respiratory syndrome (PRRS). The known disease status of sows was regressed on the traits number born alive, number of losses as a combination of still birth and mummified piglets, and number of weaned piglets. The regression coefficients from this analysis were then used as weighting factors for derivation of an index measure called challenge load indicator. These weighting factors were derived with i) a two-step approach using residuals or year-week solutions estimated from a previous step, and ii) a single-step approach using the trait values directly. Two types of models were used for each approach: a logistic regression model and a general additive model. The estimates of challenge load indicator were then compared based on their ability to detect PRRS outbreaks in a Test dataset consisting of records from 65,826 sows from 15 farms in the Netherlands. These farms differed from the Canadian farm with respect to PRRS virus strains, severity and frequency of outbreaks. The single-step approach using a general additive model was best and detected 14 out of the 15 outbreaks. This approach was then further validated using the third dataset consisting of reproduction records of 831,855 sows in 431 farms located in different countries in Europe and America. A total of 41 out of 48 outbreaks detected

Multiple endocrine neoplasia type 2: achievements and current challenges

Directory of Open Access Journals (Sweden)

Andreas Machens

2012-01-01

Full Text Available Incremental advances in medical technology, such as the development of sensitive hormonal assays for routine clinical care, are the drivers of medical progress. This principle is exemplified by the creation of the concept of multiple endocrine neoplasia type 2, encompassing medullary thyroid cancer, pheochromocytoma, and primary hyperparathyroidism, which did not emerge before the early 1960s. This review sets out to highlight key achievements, such as joint biochemical and DNA-based screening of individuals at risk of developing multiple endocrine neoplasia type 2, before casting a spotlight on current challenges which include: (i ill-defined upper limits of calcitonin assays for infants and young children, rendering it difficult to implement the biochemical part of the integrated DNA-based/biochemical concept; (ii our increasingly mobile society in which different service providers are caring for one individual at various stages in the disease process. With familial relationships disintegrating as a result of geographic dispersion, information about the history of the origin family may become sketchy or just unavailable. This is when DNA-based gene tests come into play, confirming or excluding an individual's genetic predisposition to multiple endocrine neoplasia type 2 even before there is any biochemical or clinical evidence of the disease. However, the unrivaled molecular genetic progress in multiple endocrine neoplasia type 2 does not come without a price. Screening may uncover unknown gene sequence variants representing either harmless polymorphisms or pathogenic mutations. In this setting, functional characterization of mutant cells in vitro may generate helpful ancillary evidence with regard to the pathogenicity of gene variants in comparison with established mutations.

Challenges of E-learning in medicine: methods and results of a systematical exploration

Directory of Open Access Journals (Sweden)

Spreckelsen, Cord

2006-11-01

Full Text Available E-learning in medicine traditionally concentrates on case oriented or problem oriented learning scenarios, the development of multimedia courseware or the implementation of simulators. This paper aims at a systematic exploration of actual and new challenges for E-learning in the medical domain. The exploration is based on the analysis of the scientific discourse in the field of Medical Education. The analysis starts from text based sources: the concept hierarchy of the Medical Subject Headings, the profiles of the relevant scientific associations, and the scientific program of scientific conferences or annual meetings. These sources are subjected to conceptual analysis, supported by network visualization tools and supplemented by network theoretic indices (Betweeness Centrality. As a result, the main concerns of the Medical Education community and their modifications during the last six years can be identified. The analysis discovers new challenges, which result from central issues of Medical Education, namely from e.g. curricular and faculty development or the sustainable integration of postgraduate education and continuing medial education. The main challenges are: 1 the implementation of integrative conceptions of the application of learning management systems (LMS and 2 the necessity of combining aspects of organizational development, knowledge management and learning management within the scope of a comprehensive learning life cycle management.

Opportunities and Challenges in the Design and Analysis of Biomass Supply Chains

Science.gov (United States)

Lautala, Pasi T.; Hilliard, Michael R.; Webb, Erin; Busch, Ingrid; Richard Hess, J.; Roni, Mohammad S.; Hilbert, Jorge; Handler, Robert M.; Bittencourt, Roger; Valente, Amir; Laitinen, Tuuli

2015-12-01

The biomass supply chain is one of the most critical elements of large-scale bioenergy production and in many cases a key barrier for procuring initial funding for new developments on specific energy crops. Most productions rely on complex transforming chains linked to feed and food markets. The term `supply chain' covers various aspects from cultivation and harvesting of the biomass, to treatment, transportation, and storage. After energy conversion, the product must be delivered to final consumption, whether it is in the form of electricity, heat, or more tangible products, such as pellets and biofuels. Effective supply chains are of utmost importance for bioenergy production, as biomass tends to possess challenging seasonal production cycles and low mass, energy and bulk densities. Additionally, the demand for final products is often also dispersed, further complicating the supply chain. The goal of this paper is to introduce key components of biomass supply chains, examples of related modeling applications, and if/how they address aspects related to environmental metrics and management. The paper will introduce a concept of integrated supply systems for sustainable biomass trade and the factors influencing the bioenergy supply chain landscape, including models that can be used to investigate the factors. The paper will also cover various aspects of transportation logistics, ranging from alternative modal and multi-modal alternatives to introduction of support tools for transportation analysis. Finally gaps and challenges in supply chain research are identified and used to outline research recommendations for the future direction in this area of study.

Opportunities and Challenges in the Design and Analysis of Biomass Supply Chains.

Science.gov (United States)

Lautala, Pasi T; Hilliard, Michael R; Webb, Erin; Busch, Ingrid; Richard Hess, J; Roni, Mohammad S; Hilbert, Jorge; Handler, Robert M; Bittencourt, Roger; Valente, Amir; Laitinen, Tuuli

2015-12-01

The biomass supply chain is one of the most critical elements of large-scale bioenergy production and in many cases a key barrier for procuring initial funding for new developments on specific energy crops. Most productions rely on complex transforming chains linked to feed and food markets. The term 'supply chain' covers various aspects from cultivation and harvesting of the biomass, to treatment, transportation, and storage. After energy conversion, the product must be delivered to final consumption, whether it is in the form of electricity, heat, or more tangible products, such as pellets and biofuels. Effective supply chains are of utmost importance for bioenergy production, as biomass tends to possess challenging seasonal production cycles and low mass, energy and bulk densities. Additionally, the demand for final products is often also dispersed, further complicating the supply chain. The goal of this paper is to introduce key components of biomass supply chains, examples of related modeling applications, and if/how they address aspects related to environmental metrics and management. The paper will introduce a concept of integrated supply systems for sustainable biomass trade and the factors influencing the bioenergy supply chain landscape, including models that can be used to investigate the factors. The paper will also cover various aspects of transportation logistics, ranging from alternative modal and multi-modal alternatives to introduction of support tools for transportation analysis. Finally gaps and challenges in supply chain research are identified and used to outline research recommendations for the future direction in this area of study.

Workflows and performances in the ranking prediction of 2016 D3R Grand Challenge 2: lessons learned from a collaborative effort.

Science.gov (United States)

Gao, Ying-Duo; Hu, Yuan; Crespo, Alejandro; Wang, Deping; Armacost, Kira A; Fells, James I; Fradera, Xavier; Wang, Hongwu; Wang, Huijun; Sherborne, Brad; Verras, Andreas; Peng, Zhengwei

2018-01-01

The 2016 D3R Grand Challenge 2 includes both pose and affinity or ranking predictions. This article is focused exclusively on affinity predictions submitted to the D3R challenge from a collaborative effort of the modeling and informatics group. Our submissions include ranking of 102 ligands covering 4 different chemotypes against the FXR ligand binding domain structure, and the relative binding affinity predictions of the two designated free energy subsets of 15 and 18 compounds. Using all the complex structures prepared in the same way allowed us to cover many types of workflows and compare their performances effectively. We evaluated typical workflows used in our daily structure-based design modeling support, which include docking scores, force field-based scores, QM/MM, MMGBSA, MD-MMGBSA, and MacroModel interaction energy estimations. The best performing methods for the two free energy subsets are discussed. Our results suggest that affinity ranking still remains very challenging; that the knowledge of more structural information does not necessarily yield more accurate predictions; and that visual inspection and human intervention are considerably important for ranking. Knowledge of the mode of action and protein flexibility along with visualization tools that depict polar and hydrophobic maps are very useful for visual inspection. QM/MM-based workflows were found to be powerful in affinity ranking and are encouraged to be applied more often. The standardized input and output enable systematic analysis and support methodology development and improvement for high level blinded predictions.

Workflows and performances in the ranking prediction of 2016 D3R Grand Challenge 2: lessons learned from a collaborative effort

Science.gov (United States)

Gao, Ying-Duo; Hu, Yuan; Crespo, Alejandro; Wang, Deping; Armacost, Kira A.; Fells, James I.; Fradera, Xavier; Wang, Hongwu; Wang, Huijun; Sherborne, Brad; Verras, Andreas; Peng, Zhengwei

2018-01-01

The 2016 D3R Grand Challenge 2 includes both pose and affinity or ranking predictions. This article is focused exclusively on affinity predictions submitted to the D3R challenge from a collaborative effort of the modeling and informatics group. Our submissions include ranking of 102 ligands covering 4 different chemotypes against the FXR ligand binding domain structure, and the relative binding affinity predictions of the two designated free energy subsets of 15 and 18 compounds. Using all the complex structures prepared in the same way allowed us to cover many types of workflows and compare their performances effectively. We evaluated typical workflows used in our daily structure-based design modeling support, which include docking scores, force field-based scores, QM/MM, MMGBSA, MD-MMGBSA, and MacroModel interaction energy estimations. The best performing methods for the two free energy subsets are discussed. Our results suggest that affinity ranking still remains very challenging; that the knowledge of more structural information does not necessarily yield more accurate predictions; and that visual inspection and human intervention are considerably important for ranking. Knowledge of the mode of action and protein flexibility along with visualization tools that depict polar and hydrophobic maps are very useful for visual inspection. QM/MM-based workflows were found to be powerful in affinity ranking and are encouraged to be applied more often. The standardized input and output enable systematic analysis and support methodology development and improvement for high level blinded predictions.

Quantitative analysis of Ni2+/Ni3+ in Li[NixMnyCoz]O2 cathode materials: Non-linear least-squares fitting of XPS spectra

Science.gov (United States)

Fu, Zewei; Hu, Juntao; Hu, Wenlong; Yang, Shiyu; Luo, Yunfeng

2018-05-01

Quantitative analysis of Ni2+/Ni3+ using X-ray photoelectron spectroscopy (XPS) is important for evaluating the crystal structure and electrochemical performance of Lithium-nickel-cobalt-manganese oxide (Li[NixMnyCoz]O2, NMC). However, quantitative analysis based on Gaussian/Lorentzian (G/L) peak fitting suffers from the challenges of reproducibility and effectiveness. In this study, the Ni2+ and Ni3+ standard samples and a series of NMC samples with different Ni doping levels were synthesized. The Ni2+/Ni3+ ratios in NMC were quantitatively analyzed by non-linear least-squares fitting (NLLSF). Two Ni 2p overall spectra of synthesized Li [Ni0.33Mn0.33Co0.33]O2(NMC111) and bulk LiNiO2 were used as the Ni2+ and Ni3+ reference standards. Compared to G/L peak fitting, the fitting parameters required no adjustment, meaning that the spectral fitting process was free from operator dependence and the reproducibility was improved. Comparison of residual standard deviation (STD) showed that the fitting quality of NLLSF was superior to that of G/L peaks fitting. Overall, these findings confirmed the reproducibility and effectiveness of the NLLSF method in XPS quantitative analysis of Ni2+/Ni3+ ratio in Li[NixMnyCoz]O2 cathode materials.

New challenges for text mining: mapping between text and manually curated pathways

Science.gov (United States)

Oda, Kanae; Kim, Jin-Dong; Ohta, Tomoko; Okanohara, Daisuke; Matsuzaki, Takuya; Tateisi, Yuka; Tsujii, Jun'ichi

2008-01-01

Background Associating literature with pathways poses new challenges to the Text Mining (TM) community. There are three main challenges to this task: (1) the identification of the mapping position of a specific entity or reaction in a given pathway, (2) the recognition of the causal relationships among multiple reactions, and (3) the formulation and implementation of required inferences based on biological domain knowledge. Results To address these challenges, we constructed new resources to link the text with a model pathway; they are: the GENIA pathway corpus with event annotation and NF-kB pathway. Through their detailed analysis, we address the untapped resource, ‘bio-inference,’ as well as the differences between text and pathway representation. Here, we show the precise comparisons of their representations and the nine classes of ‘bio-inference’ schemes observed in the pathway corpus. Conclusions We believe that the creation of such rich resources and their detailed analysis is the significant first step for accelerating the research of the automatic construction of pathway from text. PMID:18426550

Syndrome identification based on 2D analysis software.

Science.gov (United States)

Boehringer, Stefan; Vollmar, Tobias; Tasse, Christiane; Wurtz, Rolf P; Gillessen-Kaesbach, Gabriele; Horsthemke, Bernhard; Wieczorek, Dagmar

2006-10-01

Clinical evaluation of children with developmental delay continues to present a challenge to the clinicians. In many cases, the face provides important information to diagnose a condition. However, database support with respect to facial traits is limited at present. Computer-based analyses of 2D and 3D representations of faces have been developed, but it is unclear how well a larger number of conditions can be handled by such systems. We have therefore analysed 2D pictures of patients each being affected with one of 10 syndromes (fragile X syndrome; Cornelia de Lange syndrome; Williams-Beuren syndrome; Prader-Willi syndrome; Mucopolysaccharidosis type III; Cri-du-chat syndrome; Smith-Lemli-Opitz syndrome; Sotos syndrome; Microdeletion 22q11.2; Noonan syndrome). We can show that a classification accuracy of >75% can be achieved for a computer-based diagnosis among the 10 syndromes, which is about the same accuracy achieved for five syndromes in a previous study. Pairwise discrimination of syndromes ranges from 80 to 99%. Furthermore, we can demonstrate that the criteria used by the computer decisions match clinical observations in many cases. These findings indicate that computer-based picture analysis might be a helpful addition to existing database systems, which are meant to assist in syndrome diagnosis, especially as data acquisition is straightforward and involves off-the-shelf digital camera equipment.

Decomposition analysis of CO2 emissions from passenger cars: The cases of Greece and Denmark

International Nuclear Information System (INIS)

Papagiannaki, Katerina; Diakoulaki, Danae

2009-01-01

The paper presents a decomposition analysis of the changes in carbon dioxide (CO 2 ) emissions from passenger cars in Denmark and Greece, for the period 1990-2005. A time series analysis has been applied based on the logarithmic mean Divisia index I (LMDI I) methodology, which belongs to the wider family of index decomposition approaches. The particularity in road transport that justifies a profound analysis is its remarkably rapid growth during the last decades, followed by a respective increase in emissions. Denmark and Greece have been selected based on the challenging differences of specific socio-economic characteristics of these two small EU countries, as well as on the availability of detailed data used in the frame of the analysis. In both countries, passenger cars are responsible for half of the emissions from road transport as well as for their upward trend, which provokes the implementation of a decomposition analysis focusing exactly on this segment of road transport. The factors examined in the present decomposition analysis are related to vehicles ownership, fuel mix, annual mileage, engine capacity and technology of cars. The comparison of the results discloses the differences in the transportation profiles of the two countries and reveals how they affect the trend of CO 2 emissions.

Challenges of Ensuring Equity

DEFF Research Database (Denmark)

Pasgaard, Maya

and on a conceptual framework to examine equity in REDD+. Qualitative research with a case study in Cambodia provides the empirical foundation for the thesis, supplemented with a quantitative analysis of climate change research to address the fourth research question. Together, these articles and approaches...... these challenges, specific recomm ndations are summarized in the thesis, namely: better integration of qualitative methods in social assessments, greater emphasis on local inclusion and representativeness in relation to resource access and decision-making, more field research and cross...... social assessments in REDD+ are inadequate due to a dominant positivistic approach to methods and indicators for social assessment, which contrasts the complexities and context of REDD+ projects; 2) The distribution of costs and benefits within communities in Oddar Meanchey is uneven, as the costs...

ANALYSIS AND CHALLENGES REGARDING WOMEN CONTRIBUTION TO THE SME DEVELOPMENT IN ROMANIA

Directory of Open Access Journals (Sweden)

Ioana LUPASC

2013-12-01

Full Text Available In Europe, entrepreneurship is promoting and in SMEs are over 99%. SMEs are viewed like the “economics engine”, they are the one that are generating work places. The researches in the entrepreneurial field proved that there is a good relation between the level of the entrepreneurial activity (the frequency of which in an economy it is creating new enterprises and the economic growth. More intense the entrepreneurial activity is, the more it will have positive effects on the economy in generally. In the past years, Europe has increased interest in female entrepreneurship. This support has resulted in the creation and implementation of public policies to encourage women to engage into business, and thereby stimulate economic growth. The contribution of women in the labor market is becoming more relevant. We present analysis, challenges and characteristics of women's contribution to the development of SMEs in Romania.

Rules of Engagement: Toward an Analysis of Staff Responses to Challenging Behavior.

Science.gov (United States)

Hastings, Richard P.; Remington, Bob

1994-01-01

This article reviews literature on the responses of direct care staff to challenging behaviors of individuals with mental retardation. The paper constructs a behavior analytic description of the functions of care staff behavior in relation to their clients' challenging behaviors, draws a distinction between contingency-shaped and rule-governed…

Challenging the Standard Model with the muon g − 2

Indian Academy of Sciences (India)

The discrepancy between experiment and the Standard Model prediction of ... The measurement of the anomalous magnetic moment of the muon aµ = (g−2)/2 ( ... to evaluate the leading-order hadronic term (see [3,4] for recent reviews). .... update of their previous analysis and a new preliminary one based on data collected.

Strategies for Integrated Analysis of Genetic, Epigenetic, and Gene Expression Variation in Cancer: Addressing the Challenges

DEFF Research Database (Denmark)

Thingholm, Louise Bruun; Andersen, Lars; Makalic, Enes

2016-01-01

to integration strategies used for analyzing genetic risk factors for cancer. We critically examine the ability of these strategies to handle the complexity of the human genome and also accommodate information about the biological and functional interactions between the elements that have been measured......The development and progression of cancer, a collection of diseases with complex genetic architectures, is facilitated by the interplay of multiple etiological factors. This complexity challenges the traditional single-platform study design and calls for an integrated approach to data analysis...

Stakes and Solutions for current and up-coming Licensing Challenges in PWR and BWR Reload and Safety Analysis

Energy Technology Data Exchange (ETDEWEB)

Curca-Tiving, F.; Opel, S.

2014-07-01

Regulatory requirements for reloads and safety analyses are evolving: New safety criteria, requests for enlarged qualification databases, statistical applications, uncertainty propagation... In order to address these challenges and access more predictable licensing processes, AREVA implements a consistent code and methodology suite for PWR and BWR core design and safety analysis, based on a first principles modeling with an extremely broad international verification and validation data base. (Author)

NCBI nr-aa BLAST: CBRC-MDOM-07-0055 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-MDOM-07-0055 ref|XP_002514867.1| hydrolase, acting on ester bonds, putative [R...icinus communis] gb|EEF47421.1| hydrolase, acting on ester bonds, putative [Ricinus communis] XP_002514867.1 0.11 40% ...

NCBI nr-aa BLAST: CBRC-MDOM-08-0237 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-MDOM-08-0237 ref|XP_002514867.1| hydrolase, acting on ester bonds, putative [R...icinus communis] gb|EEF47421.1| hydrolase, acting on ester bonds, putative [Ricinus communis] XP_002514867.1 0.008 54% ...

Efficacy of a Recombinant Turkey Herpesvirus H5 Vaccine Against Challenge With H5N1 Clades 1.1.2 and 2.3.2.1 Highly Pathogenic Avian Influenza Viruses in Domestic Ducks (Anas platyrhynchos domesticus).

Science.gov (United States)

Pantin-Jackwood, Mary J; Kapczynski, Darrell R; DeJesus, Eric; Costa-Hurtado, Mar; Dauphin, Gwenaelle; Tripodi, Astrid; Dunn, John R; Swayne, David E

2016-03-01

Domestic ducks are the second most abundant poultry species in many Asian countries and have played a critical role in the epizootiology of H5N1 highly pathogenic avian influenza (HPAI).In this study, the protective efficacy of a live recombinant vector vaccine based on a turkey herpesvirus (HVT) expressing the H5 gene from a clade 2.2 H5N1 HPAI strain (A/Swan/Hungary/4999/ 2006) (rHVT-H5/2.2), given at 3 days of age, was examined in Pekin ducks (Anas platyrhynchos domesticus). The vaccine was given alone or in combination with an inactivated H5N1 clade 2.3.2.1 reverse genetic (rgGD/2.3.2.1) vaccine given at 16 days of age, either as a single vaccination or in a prime-boost regime. At 30 days of age, ducks were challenged with one of two H5N1 HPAI viruses: A/duck/Vietnam/NCVD-2721/2013 (clade 1.1.2) or A/duck/Vietnam/NCVD-1584/2012 (clade 2.3.2.1.C). These viruses produced 100% mortality in less than 5 days in nonvaccinated control ducks. Ducks vaccinated with the rgGD/2.3.2.1 vaccine, with or without the rHVT-H5/2.2 vaccine, were 90%-100% protected against mortality after challenge with either of the two H5N1 HPAI viruses. The rHVT-H5/2.2 vaccine alone, however, conferred only 30% protection against mortality after challenge with either H5N1 HPAI virus; the surviving ducks from these groups shed higher amount of virus and for longer than the single-vaccinated rgGD/2.3.2.1 group. Despite low protection, ducks vaccinated with the rHVT-H5/2.2 vaccine and challenged with the clade 1.1.2 Vietnam virus had a longer mean death time than nonvaccinated controls (P = 0.02). A booster effect was found on reduction of virus shedding when using both vaccines, with lower oropharyngeal viral titers at 4 days after challenge with either HPAI virus (P study demonstrates the suboptimal protection with the rHVT-H5/2.2 vaccine given alone in Pekin ducks against H5N1 HPAI viruses and only a minor additive effect on virus shedding reduction when used with an inactivated vaccine in a

The Adverse Drug Reactions from Patient Reports in Social Media Project: Five Major Challenges to Overcome to Operationalize Analysis and Efficiently Support Pharmacovigilance Process.

Science.gov (United States)

Bousquet, Cedric; Dahamna, Badisse; Guillemin-Lanne, Sylvie; Darmoni, Stefan J; Faviez, Carole; Huot, Charles; Katsahian, Sandrine; Leroux, Vincent; Pereira, Suzanne; Richard, Christophe; Schück, Stéphane; Souvignet, Julien; Lillo-Le Louët, Agnès; Texier, Nathalie

2017-09-21

Adverse drug reactions (ADRs) are an important cause of morbidity and mortality. Classical Pharmacovigilance process is limited by underreporting which justifies the current interest in new knowledge sources such as social media. The Adverse Drug Reactions from Patient Reports in Social Media (ADR-PRISM) project aims to extract ADRs reported by patients in these media. We identified 5 major challenges to overcome to operationalize the analysis of patient posts: (1) variable quality of information on social media, (2) guarantee of data privacy, (3) response to pharmacovigilance expert expectations, (4) identification of relevant information within Web pages, and (5) robust and evolutive architecture. This article aims to describe the current state of advancement of the ADR-PRISM project by focusing on the solutions we have chosen to address these 5 major challenges. In this article, we propose methods and describe the advancement of this project on several aspects: (1) a quality driven approach for selecting relevant social media for the extraction of knowledge on potential ADRs, (2) an assessment of ethical issues and French regulation for the analysis of data on social media, (3) an analysis of pharmacovigilance expert requirements when reviewing patient posts on the Internet, (4) an extraction method based on natural language processing, pattern based matching, and selection of relevant medical concepts in reference terminologies, and (5) specifications of a component-based architecture for the monitoring system. Considering the 5 major challenges, we (1) selected a set of 21 validated criteria for selecting social media to support the extraction of potential ADRs, (2) proposed solutions to guarantee data privacy of patients posting on Internet, (3) took into account pharmacovigilance expert requirements with use case diagrams and scenarios, (4) built domain-specific knowledge resources embeding a lexicon, morphological rules, context rules, semantic rules

A pipeline to determine RT-QPCR control genes for evolutionary studies: application to primate gene expression across multiple tissues.

Directory of Open Access Journals (Sweden)

Olivier Fedrigo

Full Text Available Because many species-specific phenotypic differences are assumed to be caused by differential regulation of gene expression, many recent investigations have focused on measuring transcript abundance. Despite the availability of high-throughput platforms, quantitative real-time polymerase chain reaction (RT-QPCR is often the method of choice because of its low cost and wider dynamic range. However, the accuracy of this technique heavily relies on the use of multiple valid control genes for normalization. We created a pipeline for choosing genes potentially useful as RT-QPCR control genes for measuring expression between human and chimpanzee samples across multiple tissues, using published microarrays and a measure of tissue-specificity. We identified 13 genes from the pipeline and from commonly used control genes: ACTB, USP49, ARGHGEF2, GSK3A, TBP, SDHA, EIF2B2, GPDH, YWHAZ, HPTR1, RPL13A, HMBS, and EEF2. We then tested these candidate genes and validated their expression stability across species. We established the rank order of the most preferable set of genes for single and combined tissues. Our results suggest that for at least three tissues (cerebral cortex, liver, and skeletal muscle, EIF2B2, EEF2, HMBS, and SDHA are useful genes for normalizing human and chimpanzee expression using RT-QPCR. Interestingly, other commonly used control genes, including TBP, GAPDH, and, especially ACTB do not perform as well. This pipeline could be easily adapted to other species for which expression data exist, providing taxonomically appropriate control genes for comparisons of gene expression among species.

Genetic variation in GIPR influences the glucose and insulin responses to an oral glucose challenge

NARCIS (Netherlands)

R. Saxena (Richa); M.-F. Hivert (Marie-France); C. Langenberg (Claudia); T. Tanaka (Toshiko); J.S. Pankow (James); P. Vollenweider (Peter); V. Lyssenko (Valeriya); N. Bouatia-Naji (Nabila); J. Dupuis (Josée); A.U. Jackson (Anne); W.H.L. Kao (Wen); M. Li (Man); N.L. Glazer (Nicole); A.K. Manning (Alisa); J. Anluan (Jian); H.M. Stringham (Heather); I. Prokopenko (Inga); T. Johnson (Toby); N. Grarup (Niels); T.W. Boesgaard (Trine); C. Lecoeur (Cécile); P. Shrader (Peter); J.R. O´Connell; E. Ingelsson (Erik); D.J. Couper (David); K. Rice (Kenneth); K. Song (Kijoung); C.H. Andreasen (Camilla); C. Dina (Christian); A. Köttgen (Anna); O.L. Bacquer (Olivier); F. Pattou (François); J. Taneera (Jalal); V. Steinthorsdottir (Valgerdur); D. Rybin (Denis); K.G. Ardlie (Kristin); M.J. Sampson (Michael); L. Qi (Lu); M.V. Hoek; M.N. Weedon (Michael); Y.S. Aulchenko (Yurii); B.F. Voight (Benjamin); H. Grallert (Harald); B. Balkau (Beverley); R.N. Bergman (Richard); S.J. Bielinski (Suzette); A. Bonnefond (Amélie); L.L. Bonnycastle (Lori); K. Borch-Johnsen; Y. Böttcher (Yvonne); E. Brunner (Eric); T.A. Buchanan (Thomas); S. Bumpstead (Suzannah); C. Cavalcanti-Proença (Christine); G. Charpentier (Guillaume); Y.D.I. Chen (Yii-Der Ida); P.S. Chines (Peter); F.S. Collins (Francis); M. Cornelis (Marilyn); G. Crawford (Gabe); J. Delplanque (Jerome); A.S.F. Doney (Alex); J.M. Egan (Josephine); M.R. Erdos (Michael); M. Firmann (Mathieu); N.G. Forouhi (Nita); C.S. Fox (Caroline); M. Goodarzi (Mark); J. Graessler (Jürgen); A. Hingorani (Aroon); B. Isomaa (Bo); T. Jørgensen (Torben); M. Kivimaki (Mika); P. Kovacs (Peter); K. Krohn (Knut); M. Kumari (Meena); T. Lauritzen (Torsten); C. Lévy-Marchal (Claire); V. Mayor (Vladimir); J.B. McAteer (Jarred); D. Meyre (David); B.D. Mitchell (Braxton); K.L. Mohlke (Karen); M.A. Morken (Mario); N. Narisu (Narisu); C.N.A. Palmer (Colin); R. Pakyz (Ruth); L. Pascoe (Laura); F. Payne (Felicity); D. Pearson (Daniel); W. Rathmann (Wolfgang); A. Sandbaek (Annelli); A.A. Sayer; L.J. Scott (Laura); S.J. Sharp (Stephen); E.J.G. Sijbrands (Eric); A. Singleton (Andrew); D.S. Siscovick (David); N.L. Smith (Nicholas); T. Sparsø (Thomas); A.J. Swift (Amy); H. Syddall (Holly); G. Thorleifsson (Gudmar); A. Tönjes (Anke); T. Tuomi (Tiinamaija); J. Tuomilehto (Jaakko); T.T. Valle (Timo); G. Waeber (Gérard); A. Walley (Andrew); D. Waterworth (Dawn); E. Zeggini (Eleftheria); J.H. Zhao (Jing Hua); G. Consortium (Giant); T. Illig (Thomas); H.E. Wichmann (Erich); J.F. Wilson (James); C.M. van Duijn (Cornelia); F.B. Hu (Frank); A.D. Morris (Andrew); T.M. Frayling (Timothy); A.T. Hattersley (Andrew); U. Thorsteinsdottir (Unnur); J-A. Zwart (John-Anker); P. Nilsson (Peter); A.C. Syvänen; A.R. Shuldiner (Alan); M. Walker (Mark); S.R. Bornstein (Stefan); P. Schwarz (Peter); G.H. Williams (Gordon); D.M. Nathan (David); J. Kuusisto (Johanna); M. Laakso (Markku); C. Cooper (Charles); M. Marmot (Michael); L. Ferrucci (Luigi); V. Mooser (Vincent); M. Stumvoll (Michael); R.J.F. Loos (Ruth); D. Altshuler (David); B.M. Psaty (Bruce); J.I. Rotter (Jerome); E.A. Boerwinkle (Eric); T. Hansen (Torben); O. Pedersen (Oluf); J.C. Florez (Jose); M.I. McCarthy (Mark); M. Boehnke (Michael); I.E. Barroso (Inês); R. Sladek (Rob); P. Froguel (Philippe); J.B. Meigs (James); L. Groop (Leif); N.J. Wareham (Nick); R.M. Watanabe (Richard)

2010-01-01

textabstractGlucose levels 2 h after an oral glucose challenge are a clinical measure of glucose tolerance used in the diagnosis of type 2 diabetes. We report a meta-analysis of nine genome-wide association studies (n = 15,234 nondiabetic individuals) and a follow-up of 29 independent loci (n =

Effect of different probiotics on breast quality characteristics of broilers under Salmonella challenge

Directory of Open Access Journals (Sweden)

Abdullah N. Al-Owaimer

2014-07-01

Full Text Available The current study was performed to investigate the influence of probiotics or antibiotic on breast quality characteristics of broiler chickens that were subjected to Salmonella challenge. Two hundred, one-day-old Cobb 500 chicks were allocated in five experimental treatments for 42 d. Ten cages of birds received one of the following treatments: T1=positive control (+CONT, unsupplemented, unchallenged; T2=negative control (-CONT, unsupplemented, challenged; T3=supplemented with antibiotic neoxyval (NEOX, challenged; T4=supplemented with probiotic Toyocerin (TOYO, challenged; and T5=supplemented with probiotic CloSTATTM (CLOS, challenged. Birds in treatments T2 to T5 were challenged with 3×109 CFU/mL of Salmonella enterica subsp. typhimurium on day 16. Nine birds per treatment were sampled at the end of the trial for breast characteristics. Overall, pH and temperature values of the breast muscle were similar among all groups tested. Cooking loss results indicated that breasts from T3 birds had the highest degree of shrinkage upon cooking while those of the probiotic group had similar control values (P<0.0001. Probiotic supplementation reduced the extent of destruction of myofibrils caused by homogenisation (P<0.0001. Warner-Bratzler shear test and texture profile analysis showed that neither treatments nor Salmonella challenge had any negative impact on texture or sensory attributes of chicken breast. In conclusion, results show that breast characteristics were better when probiotics were supplemented in the diets.

Overexpression of human virus surface glycoprotein precursors induces cytosolic unfolded protein response in Saccharomyces cerevisiae

Directory of Open Access Journals (Sweden)

Sasnauskas Kęstutis

2011-05-01

Full Text Available Abstract Background The expression of human virus surface proteins, as well as other mammalian glycoproteins, is much more efficient in cells of higher eukaryotes rather than yeasts. The limitations to high-level expression of active viral surface glycoproteins in yeast are not well understood. To identify possible bottlenecks we performed a detailed study on overexpression of recombinant mumps hemagglutinin-neuraminidase (MuHN and measles hemagglutinin (MeH in yeast Saccharomyces cerevisiae, combining the analysis of recombinant proteins with a proteomic approach. Results Overexpressed recombinant MuHN and MeH proteins were present in large aggregates, were inactive and totally insoluble under native conditions. Moreover, the majority of recombinant protein was found in immature form of non-glycosylated precursors. Fractionation of yeast lysates revealed that the core of viral surface protein aggregates consists of MuHN or MeH disulfide-linked multimers involving eukaryotic translation elongation factor 1A (eEF1A and is closely associated with small heat shock proteins (sHsps that can be removed only under denaturing conditions. Complexes of large Hsps seem to be bound to aggregate core peripherally as they can be easily removed at high salt concentrations. Proteomic analysis revealed that the accumulation of unglycosylated viral protein precursors results in specific cytosolic unfolded protein response (UPR-Cyto in yeast cells, characterized by different action and regulation of small Hsps versus large chaperones of Hsp70, Hsp90 and Hsp110 families. In contrast to most environmental stresses, in the response to synthesis of recombinant MuHN and MeH, only the large Hsps were upregulated whereas sHsps were not. Interestingly, the amount of eEF1A was also increased during this stress response. Conclusions Inefficient translocation of MuHN and MeH precursors through ER membrane is a bottleneck for high-level expression in yeast. Overexpression of

Safeguards challenges of Fast Breeder Reactor

International Nuclear Information System (INIS)

Ko, H. S.

2010-01-01

Although the safeguards system of Sodium Fast Reactor (SFR) seems similar to that of Light Water Reactor (LWR), it was raised safeguards challenges of SFR that resulted from the visual opacity of liquid sodium, chemical reactivity of sodium and other characteristics of fast reactor. As it is the basic concept stage of the safeguards of SFR in Korea, this study tried to analyze the latest similar study of safeguards issues of the Fast Breeder Reactor (FBR) at Joyo and Monju in Japan. For this reason, this study is to introduce some potential safeguards challenges of Fast Breeder Reactor. With this analysis, future study could be to address the safeguards challenges of SFR in Korea

The Decomposition Analysis of CO2 Emission and Economic Growth in Pakistan India and China

Directory of Open Access Journals (Sweden)

Muhammad Irfan Javaid Attari

2011-12-01

Full Text Available The conflict between economic growth and keeping greenhouse gases (GHG at controllable levels is one of the ultimate challenges of this century. The aim of Kyoto Protocol is to keep the level of carbon dioxide (CO2 below a certain threshold level. The purpose of this paper is to study the effect of CO2 emission on economic growth by conducting the regional analysis of PIC nations i.e. Pakistan, India and China. The study also provides the detail information regarding the atmospheric emission by applying decomposition analysis. It is suggested that environmental policies need more attention in the region by keeping the differences aside. So, the emission trading is considered to be the new concept. The approach should be introduced to tackle down the global warming in the region. Now it is time to respond because the low Carbon Economy is the reality.

Toxaphene: a challenging analytical problem

NARCIS (Netherlands)

de Geus, H.J.; Wester, P.G.; Schelvis, A.; de Boer, J.; Brinkman, U.A.T.

2000-01-01

The analysis of toxaphene, a highly complex mixture of chlorinated bornanes, bornenes and camphenes, is a challenging problem, especially as individual congeners are present at trace levels in biota and other relevant samples. The complicated nomenclature of the compounds of interest is briefly

Progress and challenges in the development and qualification of multi-level multi-physics coupled methodologies for reactor analysis

International Nuclear Information System (INIS)

Ivanov, K.; Avramova, M.

2007-01-01

Current trends in nuclear power generation and regulation as well as the design of next generation reactor concepts along with the continuing computer technology progress stimulate the development, qualification and application of multi-physics multi-scale coupled code systems. The efforts have been focused on extending the analysis capabilities by coupling models, which simulate different phenomena or system components, as well as on refining the scale and level of detail of the coupling. This paper reviews the progress made in this area and outlines the remaining challenges. The discussion is illustrated with examples based on neutronics/thermohydraulics coupling in the reactor core modeling. In both fields recent advances and developments are towards more physics-based high-fidelity simulations, which require implementation of improved and flexible coupling methodologies. First, the progresses in coupling of different physics codes along with the advances in multi-level techniques for coupled code simulations are discussed. Second, the issues related to the consistent qualification of coupled multi-physics and multi-scale code systems for design and safety evaluation are presented. The increased importance of uncertainty and sensitivity analysis are discussed along with approaches to propagate the uncertainty quantification between the codes. The incoming OECD LWR Uncertainty Analysis in Modeling (UAM) benchmark is the first international activity to address this issue and it is described in the paper. Finally, the remaining challenges with multi-physics coupling are outlined. (authors)

Progress and challenges in the development and qualification of multi-level multi-physics coupled methodologies for reactor analysis

Energy Technology Data Exchange (ETDEWEB)

Ivanov, K.; Avramova, M. [Pennsylvania State Univ., University Park, PA (United States)

2007-07-01

Current trends in nuclear power generation and regulation as well as the design of next generation reactor concepts along with the continuing computer technology progress stimulate the development, qualification and application of multi-physics multi-scale coupled code systems. The efforts have been focused on extending the analysis capabilities by coupling models, which simulate different phenomena or system components, as well as on refining the scale and level of detail of the coupling. This paper reviews the progress made in this area and outlines the remaining challenges. The discussion is illustrated with examples based on neutronics/thermohydraulics coupling in the reactor core modeling. In both fields recent advances and developments are towards more physics-based high-fidelity simulations, which require implementation of improved and flexible coupling methodologies. First, the progresses in coupling of different physics codes along with the advances in multi-level techniques for coupled code simulations are discussed. Second, the issues related to the consistent qualification of coupled multi-physics and multi-scale code systems for design and safety evaluation are presented. The increased importance of uncertainty and sensitivity analysis are discussed along with approaches to propagate the uncertainty quantification between the codes. The incoming OECD LWR Uncertainty Analysis in Modeling (UAM) benchmark is the first international activity to address this issue and it is described in the paper. Finally, the remaining challenges with multi-physics coupling are outlined. (authors)

Effective Analysis of NGS Metagenomic Data with Ultra-Fast Clustering Algorithms (MICW - Metagenomics Informatics Challenges Workshop: 10K Genomes at a Time)

Energy Technology Data Exchange (ETDEWEB)

Li, Weizhong

2011-10-12

San Diego Supercomputer Center's Weizhong Li on "Effective Analysis of NGS Metagenomic Data with Ultra-fast Clustering Algorithms" at the Metagenomics Informatics Challenges Workshop held at the DOE JGI on October 12-13, 2011.

Session Introduction: Challenges of Pattern Recognition in Biomedical Data.

Science.gov (United States)

Verma, Shefali Setia; Verma, Anurag; Basile, Anna Okula; Bishop, Marta-Byrska; Darabos, Christian

2018-01-01

The analysis of large biomedical data often presents with various challenges related to not just the size of the data, but also to data quality issues such as heterogeneity, multidimensionality, noisiness, and incompleteness of the data. The data-intensive nature of computational genomics problems in biomedical informatics warrants the development and use of massive computer infrastructure and advanced software tools and platforms, including but not limited to the use of cloud computing. Our session aims to address these challenges in handling big data for designing a study, performing analysis, and interpreting outcomes of these analyses. These challenges have been prevalent in many studies including those which focus on the identification of novel genetic variant-phenotype associations using data from sources like Electronic Health Records (EHRs) or multi-omic data. One of the biggest challenges to focus on is the imperfect nature of the biomedical data where a lot of noise and sparseness is observed. In our session, we will present research articles that can help in identifying innovative ways to recognize and overcome newly arising challenges associated with pattern recognition in biomedical data.

Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge.

Science.gov (United States)

Huang, Jingwei; Zhang, Zhenchao; Li, Menghui; Song, Xiaokai; Yan, Ruofeng; Xu, Lixin; Li, Xiangrui

2015-10-01

E. maxima is one of the seven species of Eimeria that infects chicken. Until now, only a few antigenic genes of E. maxima have been reported. In the present study, the immune protective effects against E. maxima challenge of recombinant protein and DNA vaccine encoding EmMIC2 were evaluated. Two-week-old chickens were randomly divided into five groups. The experimental group of chickens was immunized with 100 μg DNA vaccine pVAX1-MIC2 or 200 μg rEmMIC2 protein while the control group of chickens was injected with pVAX1 plasmid or sterile PBS. The results showed that the anti-EmMIC2 antibody titers of both rEmMIC2 protein and pVAX1-MIC2 groups were significantly higher as compared to PBS and pVAX1 control (Pmaxima challenge and it could be an effective antigen candidate for the development of new vaccines against E. maxima. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Analysis of metabolomic data: tools, current strategies and future challenges for omics data integration.

Science.gov (United States)

Cambiaghi, Alice; Ferrario, Manuela; Masseroli, Marco

2017-05-01

Metabolomics is a rapidly growing field consisting of the analysis of a large number of metabolites at a system scale. The two major goals of metabolomics are the identification of the metabolites characterizing each organism state and the measurement of their dynamics under different situations (e.g. pathological conditions, environmental factors). Knowledge about metabolites is crucial for the understanding of most cellular phenomena, but this information alone is not sufficient to gain a comprehensive view of all the biological processes involved. Integrated approaches combining metabolomics with transcriptomics and proteomics are thus required to obtain much deeper insights than any of these techniques alone. Although this information is available, multilevel integration of different 'omics' data is still a challenge. The handling, processing, analysis and integration of these data require specialized mathematical, statistical and bioinformatics tools, and several technical problems hampering a rapid progress in the field exist. Here, we review four main tools for number of users or provided features (MetaCoreTM, MetaboAnalyst, InCroMAP and 3Omics) out of the several available for metabolomic data analysis and integration with other 'omics' data, highlighting their strong and weak aspects; a number of related issues affecting data analysis and integration are also identified and discussed. Overall, we provide an objective description of how some of the main currently available software packages work, which may help the experimental practitioner in the choice of a robust pipeline for metabolomic data analysis and integration. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

System Engineering and Technical Challenges Overcome in the J-2X Rocket Engine Development Project

Science.gov (United States)

Ballard, Richard O.

2012-01-01

Beginning in 2006, NASA initiated the J-2X engine development effort to develop an upper stage propulsion system to enable the achievement of the primary objectives of the Constellation program (CxP): provide continued access to the International Space Station following the retirement of the Space Station and return humans to the moon. The J-2X system requirements identified to accomplish this were very challenging and the time expended over the five years following the beginning of the J- 2X effort have been noteworthy in the development of innovations in both the fields for liquid rocket propulsion and system engineering.

Modeling energy consumption and CO2 emissions at the urban scale: Methodological challenges and insights from the United States

International Nuclear Information System (INIS)

Parshall, Lily; Gurney, Kevin; Hammer, Stephen A.; Mendoza, Daniel; Zhou, Yuyu; Geethakumar, Sarath

2010-01-01

Local policy makers could benefit from a national, high-resolution inventory of energy consumption and related carbon dioxide (CO 2 ) emissions based on the Vulcan data product, which plots emissions on a 100 km 2 grid. We evaluate the ability of Vulcan to measure energy consumption in urban areas, a scale of analysis required to support goals established as part of local energy, climate or sustainability initiatives. We highlight the methodological challenges of this type of analytical exercise and review alternative approaches. We find that between 37% and 86% of direct fuel consumption in buildings and industry and between 37% and 77% of on-road gasoline and diesel consumption occurs in urban areas, depending on how these areas are defined. We suggest that a county-based definition of urban is preferable to other common definitions since counties are the smallest political unit for which energy data are collected. Urban counties, account for 37% of direct energy consumption, or 50% if mixed urban counties are included. A county-based definition can also improve estimates of per-capita consumption.

The Fire Challenge: A Case Report and Analysis of Self-Inflicted Flame Injury Posted on Social Media.

Science.gov (United States)

Avery, Andrew H; Rae, Lisa; Summitt, J Blair; Kahn, Steven Alexander

2016-01-01

With the advent of social media platforms such as Facebook and YouTube, online dissemination of exhibitionist videos has gained popularity. One recent disturbing trend is the "fire challenge" wherein a participant douses his or herself in a household accelerant such as isopropyl alcohol or acetone, sets him or herself ablaze, and attempts to extinguish the flames before serious burns are incurred. As expected, participants in the "fire challenge" often accidentally suffer serious burns. A 17-year-old white male was recently treated at our burn center after participating in the "fire challenge." He suffered 15% TBSA full and partial thickness burns requiring split thickness skin grafting to his abdomen. He reported lighting himself on fire because he had seen this stunt performed on the internet. A search for "fire challenge" and similar terms was conducted on YouTube (www.youtube.com). Gender and ethnicity of each participant were documented. Burn size, burn depth, and age of video participant were estimated by two attending burn surgeons evaluating YouTube videos. Results were reported with descriptive statistics. The search yielded thousands of hits, mostly home videos, compilations of stunts, and commentaries. After omitting duplicate and irrelevant videos, 50 videos were selected for the study. Of these, 13 videos included postburn footage demonstrating burn wounds of various location, size, and severity. Of these burns, the median TBSA burned was 4 ± 2.7% with a maximum size of 10%. Superficial and partial thickness burns were sustained on the torso (10/13, 77%), face (4/13, 31%), and extremities (2/13, 15%). Full thickness burns were seen in 2/13 videos. Some burn wounds were obscured by dressings. Of the 50 videos reviewed, 45/50 participants (90%) were male and 32/50 (64%) were African American with 29/50 participants (58%) estimated to be under age 20. The "fire challenge" is a popular social media phenomenon, but it can result in severe injury as seen

Challenges and opportunities of airborne metagenomics.

Science.gov (United States)

Behzad, Hayedeh; Gojobori, Takashi; Mineta, Katsuhiko

2015-05-06

Recent metagenomic studies of environments, such as marine and soil, have significantly enhanced our understanding of the diverse microbial communities living in these habitats and their essential roles in sustaining vast ecosystems. The increase in the number of publications related to soil and marine metagenomics is in sharp contrast to those of air, yet airborne microbes are thought to have significant impacts on many aspects of our lives from their potential roles in atmospheric events such as cloud formation, precipitation, and atmospheric chemistry to their major impact on human health. In this review, we will discuss the current progress in airborne metagenomics, with a special focus on exploring the challenges and opportunities of undertaking such studies. The main challenges of conducting metagenomic studies of airborne microbes are as follows: 1) Low density of microorganisms in the air, 2) efficient retrieval of microorganisms from the air, 3) variability in airborne microbial community composition, 4) the lack of standardized protocols and methodologies, and 5) DNA sequencing and bioinformatics-related challenges. Overcoming these challenges could provide the groundwork for comprehensive analysis of airborne microbes and their potential impact on the atmosphere, global climate, and our health. Metagenomic studies offer a unique opportunity to examine viral and bacterial diversity in the air and monitor their spread locally or across the globe, including threats from pathogenic microorganisms. Airborne metagenomic studies could also lead to discoveries of novel genes and metabolic pathways relevant to meteorological and industrial applications, environmental bioremediation, and biogeochemical cycles. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

Teacher Supervision and Evaluation Challenges: Canadian Perspectives on Overall Instructional Leadership

Science.gov (United States)

Brandon, Jim; Hollweck, Trista; Donlevy, James Kent; Whalen, Catherine

2018-01-01

This inquiry focuses on the "overall instructional leadership" approaches used by exemplary principals in three high performing Canadian provinces to overcome three persistent obstacles to effective teacher supervision and evaluation: (a) the management challenge, (b) the complexity challenge, and (c) the learning challenge. Analysis of…

Administrative Challenges to the Integration of Oral Health With Primary Care: A SWOT Analysis of Health Care Executives at Federally Qualified Health Centers.

Science.gov (United States)

Norwood, Connor W; Maxey, Hannah L; Randolph, Courtney; Gano, Laura; Kochhar, Komal

Inadequate access to preventive oral health services contributes to oral health disparities and is a major public health concern in the United States. Federally Qualified Health Centers play a critical role in improving access to care for populations affected by oral health disparities but face a number of administrative challenges associated with implementation of oral health integration models. We conducted a SWOT (strengths, weaknesses, opportunities, and threats) analysis with health care executives to identify strengths, weaknesses, opportunities, and threats of successful oral health integration in Federally Qualified Health Centers. Four themes were identified: (1) culture of health care organizations; (2) operations and administration; (3) finance; and (4) workforce.

Analysis of Current Visualization Techniques and Main Challenges for the Future

Directory of Open Access Journals (Sweden)

Manuel Pérez Cota

2017-08-01

Full Text Available The big amount of data generated nowadays are being used by Big Data tools to generate knowledge and to facilitate the decision-making. However, this situation creates a new challenge: how to visualize all these data without losing mid/long term crucial information. The purpose of this article is to analyze the state of the art on massive data visualization, main problems and challenges of information representation current techniques as well as the evolution of the tools and the future of them, in other words, new functionalities to offer.

Meeting the challenges in managing TMI-2 liquid waste and resolving disposal issues

International Nuclear Information System (INIS)

Schmitt, R.C.; Brown, G.R.; Ayers, A.L. Jr.

1986-01-01

This paper describes challenges of decontaminating water resulting from the accident at the Unit 2 of the Three Mile Island nuclear power station, disposing of highly contaminated prefilter liners as commercial low-level wastes, and controlling gas generation in highly loaded, disposable zeolite vessels. Disposal of EPICOR-II prefilter liners necessitated development of the first-of-a-kind, concrete high-integrity containers. Shipment of zeolite vessels for use in a research and development program required developing and testing a catalytic recombiner that controlled gases produced by radiolytic decomposition of interstitial water in the vessels

Grand Challenges of Advanced Computing for Energy Innovation Report from the Workshop Held July 31-August 2, 2012

Energy Technology Data Exchange (ETDEWEB)

Larzelere, Alex R.; Ashby, Steven F.; Christensen, Dana C.; Crawford, Dona L.; Khaleel, Mohammad A.; John, Grosh; Stults, B. Ray; Lee, Steven L.; Hammond, Steven W.; Grover, Benjamin T.; Neely, Rob; Dudney, Lee Ann; Goldstein, Noah C.; Wells, Jack; Peltz, Jim

2013-03-06

On July 31-August 2 of 2012, the U.S. Department of Energy (DOE) held a workshop entitled Grand Challenges of Advanced Computing for Energy Innovation. This workshop built on three earlier workshops that clearly identified the potential for the Department and its national laboratories to enable energy innovation. The specific goal of the workshop was to identify the key challenges that the nation must overcome to apply the full benefit of taxpayer-funded advanced computing technologies to U.S. energy innovation in the ways that the country produces, moves, stores, and uses energy. Perhaps more importantly, the workshop also developed a set of recommendations to help the Department overcome those challenges. These recommendations provide an action plan for what the Department can do in the coming years to improve the nation’s energy future.

Expressive communication of children with autism: the use of challenging behaviour.

Science.gov (United States)

Chiang, Hsu-Min

2008-11-01

There is a lack of empirical research investigating challenging behaviour in children with autism with severe speech impairments in naturalistic settings. The aim of the present study was to investigate challenging behaviour among Australian and Taiwanese children with autism who are non-verbal or have limited speech (i.e. less than five functional words) in school settings and to address the following questions: (1) What are the communicative characteristics (e.g. communicative functions, partners, activities and partner's responses) of challenging behaviour? (2) Do sample characteristics (e.g. age, severity of autism, adaptive behaviour level, the use of graphic symbols, the use of non-symbolic acts, the use of speech) associate with the amount of challenging behaviour? (3) Do cultural differences exist in communicative variables of challenging behaviour? Naturalistic observations were used to investigate challenging behaviour in children with autism. A total of 32 (17 Australian and 15 Taiwanese) children with autism who were non-verbal or had limited speech participated in the current study and each participant was videotaped for 2 h during his/her everyday school routines. Sixteen out of 32 children had challenging behaviour. Challenging behaviour was used for requesting and rejecting communicative functions. A paired-samples t-test revealed that no significant differences existed in the frequency of occurrence between request and reject. Challenging behaviour was directed to adults and peers. A paired-samples t-test revealed that challenging behaviour was directed to adults significantly more frequently than to peers. Communicative partners showed different responses (e.g. deny, no response, delivered, removed, acknowledge) to challenging behaviour. A repeated-measures analysis of variance (ANOVA) revealed a main effect for partner response. Deny was the most common response. Challenging behaviour occurred across a variety of classroom activities (e

Using Linked Electronic Health Records to Estimate Healthcare Costs: Key Challenges and Opportunities.

Science.gov (United States)

Asaria, Miqdad; Grasic, Katja; Walker, Simon

2016-02-01

This paper discusses key challenges and opportunities that arise when using linked electronic health records (EHR) in health economics and outcomes research (HEOR), with a particular focus on estimating healthcare costs. These challenges and opportunities are framed in the context of a case study modelling the costs of stable coronary artery disease in England. The challenges and opportunities discussed fall broadly into the categories of (1) handling and organising data of this size and sensitivity; (2) extracting clinical endpoints from datasets that have not been designed and collected with such endpoints in mind; and (3) the principles and practice of costing resource use from routinely collected data. We find that there are a number of new challenges and opportunities that arise when working with EHR compared with more traditional sources of data for HEOR. These call for greater clinician involvement and intelligent use of sensitivity analysis.

Challenges in the size analysis of a silica nanoparticle mixture as candidate certified reference material

International Nuclear Information System (INIS)

Kestens, Vikram; Roebben, Gert; Herrmann, Jan; Jämting, Åsa; Coleman, Victoria; Minelli, Caterina; Clifford, Charles; Temmerman, Pieter-Jan De; Mast, Jan; Junjie, Liu; Babick, Frank; Cölfen, Helmut; Emons, Hendrik

2016-01-01

A new certified reference material for quality control of nanoparticle size analysis methods has been developed and produced by the Institute for Reference Materials and Measurements of the European Commission’s Joint Research Centre. The material, ERM-FD102, consists of an aqueous suspension of a mixture of silica nanoparticle populations of distinct particle size and origin. The characterisation relied on an interlaboratory comparison study in which 30 laboratories of demonstrated competence participated with a variety of techniques for particle size analysis. After scrutinising the received datasets, certified and indicative values for different method-defined equivalent diameters that are specific for dynamic light scattering (DLS), centrifugal liquid sedimentation (CLS), scanning and transmission electron microscopy (SEM and TEM), atomic force microscopy (AFM), particle tracking analysis (PTA) and asymmetrical-flow field-flow fractionation (AF4) were assigned. The value assignment was a particular challenge because metrological concepts were not always interpreted uniformly across all participating laboratories. This paper presents the main elements and results of the ERM-FD102 characterisation study and discusses in particular the key issues of measurand definition and the estimation of measurement uncertainty.

Challenges in the size analysis of a silica nanoparticle mixture as candidate certified reference material

Energy Technology Data Exchange (ETDEWEB)

Kestens, Vikram, E-mail: vikram.kestens@ec.europa.eu; Roebben, Gert [Joint Research Centre (JRC), European Commission, Institute for Reference Materials and Measurements (IRMM) (Belgium); Herrmann, Jan; Jämting, Åsa; Coleman, Victoria [National Measurement Institute Australia, Nanometrology Section (Australia); Minelli, Caterina; Clifford, Charles [National Physical Laboratory, Analytical Science Division (United Kingdom); Temmerman, Pieter-Jan De; Mast, Jan [Service Electron Microscopy, Veterinary and Agrochemical Research Centre (CODA-CERVA) (Belgium); Junjie, Liu [National Institute of Metrology, Division of Nanoscale Measurement and Advanced Materials (China); Babick, Frank [Technische Universität Dresden, Institut für Verfahrens- und Umwelttechnik (Germany); Cölfen, Helmut [University of Konstanz, Physical Chemistry, Department of Chemistry (Germany); Emons, Hendrik [Joint Research Centre (JRC), European Commission, Institute for Reference Materials and Measurements (IRMM) (Belgium)

2016-06-15

A new certified reference material for quality control of nanoparticle size analysis methods has been developed and produced by the Institute for Reference Materials and Measurements of the European Commission’s Joint Research Centre. The material, ERM-FD102, consists of an aqueous suspension of a mixture of silica nanoparticle populations of distinct particle size and origin. The characterisation relied on an interlaboratory comparison study in which 30 laboratories of demonstrated competence participated with a variety of techniques for particle size analysis. After scrutinising the received datasets, certified and indicative values for different method-defined equivalent diameters that are specific for dynamic light scattering (DLS), centrifugal liquid sedimentation (CLS), scanning and transmission electron microscopy (SEM and TEM), atomic force microscopy (AFM), particle tracking analysis (PTA) and asymmetrical-flow field-flow fractionation (AF4) were assigned. The value assignment was a particular challenge because metrological concepts were not always interpreted uniformly across all participating laboratories. This paper presents the main elements and results of the ERM-FD102 characterisation study and discusses in particular the key issues of measurand definition and the estimation of measurement uncertainty.

People with disabilities and work inclusion in Latin America: challenges to the universalization of rights

Directory of Open Access Journals (Sweden)

Wederson Santos

2017-12-01

Full Text Available Introduction: Occupational inclusion is one of the major contemporary challenges in promoting the human rights of people with disabilities in any country. Objective: To carry out a theoretical discussion and analysis of public policy through the characteristics of the legislative framework of the Latin American countries with their respective initiatives, policies and programs to promote the labor inclusion of people with disabilities. Method: The documents were analyzed, with emphasis on content analysis, in order to form a analysis framework on the main characteristics, similarities and challenges faced by these countries. Results: The main initiatives of the Latin American countries have been: 1. approval of legislation with mandatory vacancies in companies and public service; 2. creation of national computerized occupational intermediation systems; 3. Entrepreneurship of people with disabilities and 4. policies for personalized follow-up of the labor inclusion itinerary. Conclusion: The main challenges common to Latin American countries, which become barriers to inclusion, are: 1. To comply with the quotas reserved and to carry out supervision; 2. To increase the educational and professional qualification of the people; 3. To reconcile benefits of non-contributory / social security incomes with occupational contributions, 4. Raise awareness among businesses, families and society about the productivity and capacities of people with disabilities, and 5. Create tax exemptions and other incentives for companies to hire people with disabilities.

Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes

DEFF Research Database (Denmark)

Thomassen, Martin; Gunnarsson, Thomas Gunnar Petursson; Christensen, Peter Møller

2016-01-01

and phosphorylation were determined by western blotting. Expression of FXYD1 (30%), actin (40%), mTOR (12%), PLN (16%) and CaMKII γ/δ (25%) was higher (P... and during exercise, mainly achieved by an increased FXYD1 ser68 phosphorylation, compared to before the intervention. CaMKII thr287 and eEF2 thr56 phosphorylation at rest and during exercise, overall PKCα/β thr638/641 and mTOR ser2448 phosphorylation during repeated intense exercise as well as resting PLN...

Challenges in coupled thermal-hydraulics and neutronics simulations for LWR safety analysis

International Nuclear Information System (INIS)

Ivanov, Kostadin; Avramova, Maria

2007-01-01

The simulation of nuclear power plant accident conditions requires three-dimensional (3D) modeling of the reactor core to ensure a realistic description of physical phenomena. The operational flexibility of Light Water Reactor (LWR) plants can be improved by utilizing accurate 3D coupled neutronics/thermal-hydraulics calculations for safety margins evaluations. There are certain requirements to the coupling of thermal-hydraulic system codes and neutron-kinetics codes that ought to be considered. The objective of these requirements is to provide accurate solutions in a reasonable amount of CPU time in coupled simulations of detailed operational transient and accident scenarios. These requirements are met by the development and implementation of six basic components of the coupling methodologies: ways of coupling (internal or external coupling); coupling approach (integration algorithm or parallel processing); spatial mesh overlays; coupled time-step algorithms; coupling numerics (explicit, semi-implicit and implicit schemes); and coupled convergence schemes. These principles of the coupled simulations are discussed in details along with the scientific issues associated with the development of appropriate neutron cross-section libraries for coupled code transient modeling. The current trends in LWR nuclear power generation and regulation as well as the design of next generation LWR reactor concepts along with the continuing computer technology progress stimulate further development of these coupled code systems. These efforts have been focused towards extending the analysis capabilities as well as refining the scale and level of detail of the coupling. This article analyses the coupled phenomena and modeling challenges on both global (assembly-wise) and local (pin-wise) levels. The issues related to the consistent qualification of coupled code systems as well as their application to different types of LWR transients are presented. Finally, the advances in numerical

Challenges for Youth Employment in Pakistan : Are They Youth-Specific?

OpenAIRE

Hou, Xiaohui

2011-01-01

This paper analyzes the patterns of and the challenges for youth employment in Pakistan and examines whether these challenges are youth-specific. Using the 2005/2006 Labor Force Survey, the analysis includes determinants of unemployment, determinants of working in the formal sector, rate of return on education, and determinants of working hours. The paper finds that many of the challenges ...

Challenges in covering health disparities in local news media: an exploratory analysis assessing views of journalists.

Science.gov (United States)

Wallington, Sherrie Flynt; Blake, Kelly D; Taylor-Clark, Kalahn; Viswanath, K

2010-10-01

News coverage of health topics influences knowledge, attitudes, and behaviors at the individual level, and agendas and actions at the institutional and policy levels. Because disparities in health often are the result of social inequalities that require community-level or policy-level solutions, news stories employing a health disparities news frame may contribute to agenda-setting among opinion leaders and policymakers and lead to policy efforts aimed at reducing health disparities. This study objective was to conduct an exploratory analysis to qualitatively describe barriers that health journalists face when covering health disparities in local media. Between June and October 2007, 18 journalists from television, print, and radio in Boston, Lawrence, and Worcester, Massachusetts, were recruited using a purposive sampling technique. In-depth, semi-structured interviews were conducted by telephone, and the crystallization/immersion method was used to conduct a qualitative analysis of interview transcripts. Our results revealed that journalists said that they consider several angles when developing health stories, including public impact and personal behavior change. Challenges to employing a health disparities frame included inability to translate how research findings may impact different socioeconomic groups, and difficulty understanding how findings may translate across racial/ethnic groups. Several journalists reported that disparities-focused stories are "less palatable" for some audiences. This exploratory study offers insights into the challenges that local news media face in using health disparities news frames in their routine coverage of health news. Public health practitioners may use these findings to inform communication efforts with local media in order to advance the public dialogue about health disparities.

Challenges of the science data processing, analysis and archiving approach in BepiColombo

Science.gov (United States)

Martinez, Santa

BepiColombo is a joint mission of the European Space Agency (ESA) and the Japan Aerospace Exploration Agency (JAXA) to the planet Mercury. It comprises two separate orbiters: the Mercury Planetary Orbiter (MPO) and the Mercury Magnetospheric Orbiter (MMO). After approximately 7.5 years of cruise, BepiColombo will arrive at Mercury in 2024 and will gather data during a 1-year nominal mission, with a possible 1-year extension. The approach selected for BepiColombo for the processing, analysis and archiving of the science data represents a significant change with respect to previous ESA planetary missions. Traditionally Instrument Teams are responsible for processing, analysing and preparing their science data for the long-term archive, however in BepiColombo, the Science Ground Segment (SGS), located in Madrid, Spain, will play a key role in these activities. Fundamental aspects of this approach include: the involvement of the SGS in the definition, development and operation of the instrument processing pipelines; the production of ready-to-archive science products compatible with NASA’s Planetary Data System (PDS) standards in all the processing steps; the joint development of a quick-look analysis system to monitor deviations between planned and executed observations to feed back the results into the different planning cycles when possible; and a mission archive providing access to the scientific products and to the operational data throughout the different phases of the mission (from the early development phase to the legacy phase). In order to achieve these goals, the SGS will need to overcome a number of challenges. The proposed approach requires a flexible infrastructure able to cope with a distributed data processing system, residing in different locations but designed as a single entity. For this, all aspects related to the integration of software developed by different Instrument Teams and the alignment of their development schedules will need to be

Nursing staff stress from challenging behaviour of residents with dementia: a concept analysis

NARCIS (Netherlands)

Hazelhof, T.J.G.M.; Schoonhoven, L.; Gaal, B.G. van; Koopmans, R.T.C.M.; Gerritsen, D.L.

2016-01-01

AIM: Provide insight into the concept of stress in the context of challenging behaviour of nursing home residents with dementia and its causes and consequences. BACKGROUND: Challenging behaviour is frequent in residents with dementia, but consequences for nursing staff are unclear. INTRODUCTION:

H2@Scale Analysis

Energy Technology Data Exchange (ETDEWEB)

Ruth, Mark

2017-07-12

'H2@Scale' is a concept based on the opportunity for hydrogen to act as an intermediate between energy sources and uses. Hydrogen has the potential to be used like the primary intermediate in use today, electricity, because it too is fungible. This presentation summarizes the H2@Scale analysis efforts performed during the first third of 2017. Results of technical potential uses and supply options are summarized and show that the technical potential demand for hydrogen is 60 million metric tons per year and that the U.S. has sufficient domestic resources to meet that demand. A high level infrastructure analysis is also presented that shows an 85% increase in energy on the grid if all hydrogen is produced from grid electricity. However, a preliminary spatial assessment shows that supply is sufficient in most counties across the U.S. The presentation also shows plans for analysis of the economic potential for the H2@Scale concept. Those plans involve developing supply and demand curves for potential hydrogen generation options and as compared to other options for use of that hydrogen.

Epistemic risks: challenges in assessment and decision making

DEFF Research Database (Denmark)

Kozine, Igor

. There are calls to revisions ranging from the definition of risk and stretching to the use of risk analysis results in decision making. The talk will centre in answering the following questions: Why conventional approach to risk analysis is challenged? What are alternatives? How to operationalise the inclusion...... of epistemic uncertainty in risk analysis? How to make decisions based on risk analysis results?...

Challenges in software ecosystems research

NARCIS (Netherlands)

Serebrenik, A.; Mens, T.; Crnkovic, I.

2015-01-01

The paper is a meta-analysis of the research field of software ecosystems, by method of surveying 26 authors in the field. It presents a relevant list of literature and six themes in which challenges for software ecosystems can be grouped: Architecture and Design, Governance, Dynamics and Evolution,

Strategies used by nurses, academics and students to overcome intercultural communication challenges.

Science.gov (United States)

Henderson, Saras; Barker, Michelle; Mak, Anita

2016-01-01

Nurse clinicians and academics need to understand intercultural communication challenges to improve their communication skills and better support students' learning. Gaps exist in the literature regarding intercultural communication resources for students, academics and clinicians. The aim of this study was to explore the experiences of clinical nurses, nurse academics, and student nurses regarding intercultural communication challenges. Data were collected using focus group interviews with nineteen clinical facilitators (nurses who supervise nursing students in clinical practice), five clinical nurses, and ten nursing students. Seven nurse academics were interviewed via telephone. The purposive sample was drawn from a tertiary hospital and a university in Australia. Participants were invited to discuss challenging intercultural scenarios they had experienced including strategies they used to overcome such challenges. Using qualitative content analysis data were analysed resulting in four categories which were: 1) prejudice based on cultural diversity; 2) unfamiliarity with cultural boundaries; 3) stereotyping cultural behaviours; and 4) difficulty understanding English. Strategies participants used to mitigate challenges included resorting to cultural validation through alliance building, proactively seeking clarification, and acquiring cultural awareness knowledge. This study highlights intercultural challenges students, clinicians and academics face and signpost the way forward with useful strategies to better inform nurse education. Copyright © 2015 Elsevier Ltd. All rights reserved.

Restraint Procedures and Challenging Behaviours in Intellectual Disability: An Analysis of Causative Factors

Science.gov (United States)

Matson, Johnny L.; Boisjoli, Jessica A.

2009-01-01

Background: Persons with intellectual disability often evince challenging behaviours. Efforts have been underway for some time to develop prosocial or positive skill acquisition treatments to address challenging behaviours. However, physical/mechanical and chemical restraint is still commonly used in many clinical and community settings. Such…

TMI-2 core debris analysis

International Nuclear Information System (INIS)

Cook, B.A.; Carlson, E.R.

1985-01-01

One of the ongoing examination tasks for the damaged TMI-2 reactor is analysis of samples of debris obtained from the debris bed presently at the top of the core. This paper summarizes the results reported in the TMI-2 Core Debris Grab Sample Examination and Analysis Report, which will be available early in 1986. The sampling and analysis procedures are presented, and information is provided on the key results as they relate to the present core condition, peak temperatures during the transient, temperature history, chemical interactions, and core relocation. The results are then summarized

Management of fire and industrial safety - challenges during commissioning of a NPP

International Nuclear Information System (INIS)

Maiti, Subhaschandra; Mohan, Nalini; Ghadge, S.G.; Bajaj, S.S.

2006-01-01

Construction and commissioning period of NPP are reduced world over drastically by stringent schedule for financial and economic reasons. For meeting the schedule, commissioning of components and systems are started immediate after installation, while construction activities are continued in parallel at the same place. Parallel activities' and 'Time Constraint' have brought new challenges to 'Management of Fire and Industrial Safely' during commissioning. An innovative approach was used during such phase of commissioning of TAPP-3 and 4. This paper outlines challenges encountered during this phase and special approach and measures used to meet those challenges. This paper also outlines problems encountered during implementation of these measures and subsequent change in approach to ensure smooth and safe execution of activities. Primarily, challenges were conflicting requirements by various agencies to carryout commissioning in parallel with construction activities concurrently. Main challenges were related to fall hazard, chemical hazard, fire hazard, electrical safety, work in confined space, housekeeping problem. Moreover it was within exclusion zone of another operating plant, which added one more dimension to those challenges. Conventional Safely management approach was little short to resolve these challenges. Such challenges were envisaged; analyzed and innovative measures were arrived at. Along with conventional safely analysis like Job Safely or Hazard Analysis (JSA or JHA), Accident Analysis, Accident Trend Analysis, Fire Hazard Analysis (FHA), innovative method like 'Area-Job- Hazard Charting' and 'Ratio Analysis' were used to understand activity dependent time varying hazard scenarios. Based on this analysis, decision were taken to change various existing elements of safely management like safety organizations; standard operating procedures (SOP); emergency operating procedures (EOP); resource allocation, planning and scheduling; safely training; safely

IMMERSION AND BATH VACCINATION AGAINST ENTERIC REDMOUTH DISEASE (ERM) PROVIDES INSUFFICIENT PROTECTION AGAINST BATH CHALLENGE WITH YERSINIA RUCKERI O1 BIOTYPE 2

DEFF Research Database (Denmark)

Kragelund Strøm, Helene; Otani, Maki; Neumann, Lukas

trout stock which had been vaccinated against both Y.r. bt 1 and 2 with a commercial “state of the art” immersion vaccine and boosted with a commercial ERM oral vaccine 4 month later. The newly isolated Y.r. bt2 strain has been used to develop a standardized challenge model which gives us...... of the vaccine for 30 seconds as recommended by the manufacturer. As a positive control groups of trout were bath vaccinated for 5 minutes. Two months later was all groups bath challenged with Y.r. bt 2 in duplicate. The challenge data obtained in the present study have indicated that neither a “state of the art......” commercial nor the experimental immersion ERM vaccine provided significant protective immunity against the virulent Y.r. bt2 infections. IP. injection of the experimental vaccine without adjuvant induced full protection. Significant mortality were seen in all immersion and bath vaccinated groups...

Opportunities and challenges of Web 2.0 for vaccination decisions.

Science.gov (United States)

Betsch, Cornelia; Brewer, Noel T; Brocard, Pauline; Davies, Patrick; Gaissmaier, Wolfgang; Haase, Niels; Leask, Julie; Renkewitz, Frank; Renner, Britta; Reyna, Valerie F; Rossmann, Constanze; Sachse, Katharina; Schachinger, Alexander; Siegrist, Michael; Stryk, Marybelle

2012-05-28

A growing number of people use the Internet to obtain health information, including information about vaccines. Websites that allow and promote interaction among users are an increasingly popular source of health information. Users of such so-called Web 2.0 applications (e.g. social media), while still in the minority, represent a growing proportion of online communicators, including vocal and active anti-vaccination groups as well as public health communicators. In this paper, the authors: define Web 2.0 and examine how it may influence vaccination decisions; discuss how anti-vaccination movements use Web 2.0 as well as the challenges Web 2.0 holds for public health communicators; describe the types of information used in these different settings; introduce the theoretical background that can be used to design effective vaccination communication in a Web 2.0 environment; make recommendations for practice and pose open questions for future research. The authors conclude that, as a result of the Internet and Web 2.0, private and public concerns surrounding vaccinations have the potential to virally spread across the globe in a quick, efficient and vivid manner. Web 2.0 may influence vaccination decisions by delivering information that alters the perceived personal risk of vaccine-preventable diseases or vaccination side-effects. It appears useful for public health officials to put effort into increasing the effectiveness of existing communication by implementing interactive, customized communication. A key step to providing successful public health communication is to identify those who are particularly vulnerable to finding and using unreliable and misleading information. Thus, it appears worthwhile that public health websites strive to be easy to find, easy to use, attractive in its presentation and readily provide the information, support and advice that the searcher is looking for. This holds especially when less knowledgeable individuals are in need of reliable

CMS results in the Combined Computing Readiness Challenge CCRC'08

International Nuclear Information System (INIS)

Bonacorsi, D.; Bauerdick, L.

2009-01-01

During February and May 2008, CMS participated to the Combined Computing Readiness Challenge (CCRC'08) together with all other LHC experiments. The purpose of this worldwide exercise was to check the readiness of the Computing infrastructure for LHC data taking. Another set of major CMS tests called Computing, Software and Analysis challenge (CSA'08) - as well as CMS cosmic runs - were also running at the same time: CCRC augmented the load on computing with additional tests to validate and stress-test all CMS computing workflows at full data taking scale, also extending this to the global WLCG community. CMS exercised most aspects of the CMS computing model, with very comprehensive tests. During May 2008, CMS moved more than 3.6 Petabytes among more than 300 links in the complex Grid topology. CMS demonstrated that is able to safely move data out of CERN to the Tier-1 sites, sustaining more than 600 MB/s as a daily average for more than seven days in a row, with enough headroom and with hourly peaks of up to 1.7 GB/s. CMS ran hundreds of simultaneous jobs at each Tier-1 site, re-reconstructing and skimming hundreds of millions of events. After re-reconstruction the fresh AOD (Analysis Object Data) has to be synchronized between Tier-1 centers: CMS demonstrated that the required inter-Tier-1 transfers are achievable within a few days. CMS also showed that skimmed analysis data sets can be transferred to Tier-2 sites for analysis at sufficient rate, regionally as well as inter-regionally, achieving all goals in about 90% of >200 links. Simultaneously, CMS also ran a large Tier-2 analysis exercise, where realistic analysis jobs were submitted to a large set of Tier-2 sites by a large number of people to produce a chaotic workload across the systems, and with more than 400 analysis users in May. Taken all together, CMS routinely achieved submissions of 100k jobs/day, with peaks up to 200k jobs/day. The achieved results in CCRC'08 - focussing on the distributed

Cross-cultural research: challenge and competence.

Science.gov (United States)

Clark, Mary Jo

2012-07-01

Increasing globalization, population diversity and health disparities among non-dominant cultures necessitate cross-cultural research. Research with other cultures is fraught with challenges that must be addressed by the competent cross-cultural researcher. Areas for consideration include choice of research foci, ethical concerns, cultural adaptation of research measurements and interventions, participant recruitment and retention, strategies for data collection and analysis, dissemination of findings and perspectives of time. Approaches to dealing with these challenges are addressed, with an emphasis on community-based participatory research. © 2012 Blackwell Publishing Asia Pty Ltd.

Analysis of Challenges for Management Education in India Using Total Interpretive Structural Modelling

Science.gov (United States)

Mahajan, Ritika; Agrawal, Rajat; Sharma, Vinay; Nangia, Vinay

2016-01-01

Purpose: The purpose of this paper is to identify challenges for management education in India and explain their nature, significance and interrelations using total interpretive structural modelling (TISM), an innovative version of Warfield's interpretive structural modelling (ISM). Design/methodology/approach: The challenges have been drawn from…

ITER safety challenges and opportunities

International Nuclear Information System (INIS)

Piet, S.J.

1992-01-01

This paper reports on results of the Conceptual Design Activity (CDA) for the International Thermonuclear Experimental Reactor (ITER) suggest challenges and opportunities. ITER is capable of meeting anticipated regulatory dose limits, but proof is difficult because of large radioactive inventories needing stringent radioactivity confinement. Much research and development (R ampersand D) and design analysis is needed to establish that ITER meets regulatory requirements. There is a further oportunity to do more to prove more of fusion's potential safety and environmental advantages and maximize the amount of ITER technology on the path toward fusion power plants. To fulfill these tasks, three programmatic challenges and three technical challenges must be overcome. The first step is to fund a comprehensive safety and environmental ITER R ampersand D plan. Second is to strengthen safety and environment work and personnel in the international team. Third is to establish an external consultant group to advise the ITER Joint Team on designing ITER to meet safety requirements for siting by any of the Parties. The first of three key technical challenges is plasma engineering - burn control, plasma shutdown, disruptions, tritium burn fraction, and steady state operation. The second is the divertor, including tritium inventory, activation hazards, chemical reactions, and coolant disturbances. The third technical challenge is optimization of design requirements considering safety risk, technical risk, and cost

Globalisation and international compatibility - a challenge to ...

African Journals Online (AJOL)

The contexts of institutions for higher education are in flux with consequent learning challenges. One of these challenges is that of globalisation and the need for international compatibility. Another challenge is that Mode 2 learning programmes, material and methods need to be relevant to the specific context in which they ...

Technology 2.0: A Commentary on Progress, Challenges, and Next Steps.

Science.gov (United States)

Jones, Deborah J

2017-11-01

This commentary highlights the importance and promise of the innovative strategies described in the Child Maltreatment special issue on "Technology 2.0: A Focus on the Newest Technological Advances in Child Maltreatment Research." The commentary first highlights the collective advancements reflected in the articles in the special issue, with a primary focus on how the authors' work addresses a general challenge in services research that is perhaps nowhere more problematic than in the field of maltreatment. Next, the commentary extends the discussion of these articles to raise remaining gaps in our knowledge, theory, and methodology, which must be the focus of ongoing research if the true potential of technology as a service delivery vehicle is to be realized. Finally, the commentary concludes with a call for subsequent research which will be inspired by the articles in this special issue.

IT Challenges for Space Medicine

Science.gov (United States)

Johnson-Throop, Kathy

2010-01-01

This viewgraph presentation reviews the various Information Technology challenges for aerospace medicine. The contents include: 1) Space Medicine Activities; 2) Private Medical Information; 3) Lifetime Surveillance of Astronaut Health; 4) Mission Medical Support; 5) Data Repositories for Research; 6) Data Input and Output; 7) Finding Data/Information; 8) Summary of Challenges; and 9) Solutions and questions.

Physics challenges in the strong interactions

Energy Technology Data Exchange (ETDEWEB)

Ellis, S.D.

1991-01-01

An overview of the challenges to be faced in the area of the strong interactions during the 1990`s is presented. As an illustrative example special attention is given to the analysis of jets as studied at hadron colliders.

Physics challenges in the strong interactions

International Nuclear Information System (INIS)

Ellis, S.D.

1991-01-01

An overview of the challenges to be faced in the area of the strong interactions during the 1990's is presented. As an illustrative example special attention is given to the analysis of jets as studied at hadron colliders

Symposium on international safeguards: Addressing verification challenges. Book of extended synopses

International Nuclear Information System (INIS)

2006-01-01

A safeguards symposium has traditionally been organized by the Safeguards Department approximately every four years. The 2006 symposium addresses challenges to IAEA safeguards that have emerged or grown more serious since 2001. The increase in size and flexibility of uranium enrichment plants, for instance, and the spread of enrichment technology to a wider circle of States, pose challenges to traditional safeguards approaches. The procurement and supply networks discovered in 2004, dealing in sensitive nuclear technology and information, have serious implications for the future effectiveness of IAEA safeguards. The symposium will provide an opportunity for the IAEA and Member States to discuss options for dealing constructively with trade in sensitive nuclear technology. Reflecting developments since 2001, the 2006 symposium will focus on current challenges to the safeguards system, improving collection and analysis of safeguards information (analysis, processing tools, satellite imagery), advances in safeguards techniques and technology (future technology, neutron techniques, spent fuel verification, reprocessing, environmental sampling, containment and surveillance), further strengthening safeguards practices and approaches (safeguards approaches, integrated safeguards, R/SSAC, destructive analysis, non-destructive analysis, enrichment, reprocessing, spent fuel transfer) and future challenges. This publication contains 183 extended synopses, each of them was indexed separately

Symposium on international safeguards: Addressing verification challenges. Book of extended synopses

Energy Technology Data Exchange (ETDEWEB)

NONE

2006-07-01

A safeguards symposium has traditionally been organized by the Safeguards Department approximately every four years. The 2006 symposium addresses challenges to IAEA safeguards that have emerged or grown more serious since 2001. The increase in size and flexibility of uranium enrichment plants, for instance, and the spread of enrichment technology to a wider circle of States, pose challenges to traditional safeguards approaches. The procurement and supply networks discovered in 2004, dealing in sensitive nuclear technology and information, have serious implications for the future effectiveness of IAEA safeguards. The symposium will provide an opportunity for the IAEA and Member States to discuss options for dealing constructively with trade in sensitive nuclear technology. Reflecting developments since 2001, the 2006 symposium will focus on current challenges to the safeguards system, improving collection and analysis of safeguards information (analysis, processing tools, satellite imagery), advances in safeguards techniques and technology (future technology, neutron techniques, spent fuel verification, reprocessing, environmental sampling, containment and surveillance), further strengthening safeguards practices and approaches (safeguards approaches, integrated safeguards, R/SSAC, destructive analysis, non-destructive analysis, enrichment, reprocessing, spent fuel transfer) and future challenges. This publication contains 183 extended synopses, each of them was indexed separately.

Opportunities and Challenges for the Life Sciences Community

Science.gov (United States)

Stewart, Elizabeth; Ozdemir, Vural

2012-01-01

Abstract Twenty-first century life sciences have transformed into data-enabled (also called data-intensive, data-driven, or big data) sciences. They principally depend on data-, computation-, and instrumentation-intensive approaches to seek comprehensive understanding of complex biological processes and systems (e.g., ecosystems, complex diseases, environmental, and health challenges). Federal agencies including the National Science Foundation (NSF) have played and continue to play an exceptional leadership role by innovatively addressing the challenges of data-enabled life sciences. Yet even more is required not only to keep up with the current developments, but also to pro-actively enable future research needs. Straightforward access to data, computing, and analysis resources will enable true democratization of research competitions; thus investigators will compete based on the merits and broader impact of their ideas and approaches rather than on the scale of their institutional resources. This is the Final Report for Data-Intensive Science Workshops DISW1 and DISW2. The first NSF-funded Data Intensive Science Workshop (DISW1, Seattle, WA, September 19–20, 2010) overviewed the status of the data-enabled life sciences and identified their challenges and opportunities. This served as a baseline for the second NSF-funded DIS workshop (DISW2, Washington, DC, May 16–17, 2011). Based on the findings of DISW2 the following overarching recommendation to the NSF was proposed: establish a community alliance to be the voice and framework of the data-enabled life sciences. After this Final Report was finished, Data-Enabled Life Sciences Alliance (DELSA, www.delsall.org) was formed to become a Digital Commons for the life sciences community. PMID:22401659

The VAST Challenge: History, Scope, and Outcomes: An introduction to the Special Issue

Energy Technology Data Exchange (ETDEWEB)

Cook, Kristin A.; Grinstein, Georges; Whiting, Mark A.

2014-10-01

Visual analytics aims to facilitate human insight from complex data via a combination of visual representations, interaction techniques, and supporting algorithms. To create new tools and techniques that achieve this goal requires that researchers have an understanding of analytical questions to be addressed, data that illustrates the complexities and ambiguities found in realistic analytic settings, and methods for evaluating whether the plausible insights are gained through use of the new methods. However, researchers do not, generally speaking, have access to analysts who can articulate their problems or operational data that is used for analysis. To fill this gap, the Visual Analytics Science and Technology (VAST) Challenge has been held annually since 2006. The VAST Challenge provides an opportunity for researchers to experiment with realistic but not real problems, using realistic synthetic data with known events embedded. Since its inception, the VAST Challenge has evolved along with the visual analytics research community to pose more complex challenges, ranging from text analysis to video analysis to large scale network log analysis. The seven years of the VAST Challenge have seen advancements in research and development, education, evaluation, and in the challenge process itself. This special issue of Information Visualization highlights some of the noteworthy advancements in each of these areas. Some of these papers focus on important research questions related to the challenge itself, and other papers focus on innovative research that has been shaped by participation in the challenge. This paper describes the VAST Challenge process and benefits in detail. It also provides an introduction to and context for the remaining papers in the issue.

Cyber crime: can a standard risk analysis help in the challenges facing business continuity managers?

Science.gov (United States)

Vande Putte, Danny; Verhelst, Marc

Risk management has never been easy. Finding efficient mitigating measures is not always straightforward. Finding measures for cyber crime, however, is a really huge challenge because cyber threats are changing all the time. As the sophistication of these threats is growing, their impact increases. Moreover, society and its economy have become increasingly dependent on information and communication technologies. Standard risk analysis methodologies will help to score the cyber risk and to place it in the risk tolerance matrix. This will allow business continuity managers to figure out if there is still a gap with the maximum tolerable outage for time-critical business processes and if extra business continuity measures are necessary to fill the gap.

Eight Essential Foods in Iranian Traditional Medicine and their Role in Health Promotion and Well-being.

Science.gov (United States)

Zeinalian, Mehrdad; Eshaghi, Mehdi; Hadian, Mahdi; Naji, Homayoun; Marandi, Sayed Mohammad Masoud; Asgary, Sedigheh

2017-01-01

Eight essential foods (EEF) described in Iranian traditional medicine (ITM) have a determinant role to balance human temperament insuring health and well-being. EEF included oral, imaginary, auditory, visual, olfactory, touch, sexual, and familiarity food. Oral foods should be halal, compatible with individual temper, consumed up twice a day, and compatible with different seasons and geographic conditions. Imaginary food consists of the individual thought content which is directly related to mental and physical fitness. It helps to balance temperament if be free of negative thoughts such as suspicion and distrust to others. Auditory food includes all sounds surrounding us, some of which are sedative and help to balance temperaments, such as natural sounds, and spiritual and beautiful words. Visual food includes everything in the range of human vision which is impressive on his/her thought. Natural beautiful scenes have almost a warm temper and help to balance human temperament. Olfactory food includes odors which stimulate the smell. Touch food includes all materials in direct contact with body skin, like clothes, which have a determinant role in temper moderation in the case of being natural. Sexual food complies with the human need to express his/her love and/or is loved, so its fulfillment could prevent human mal-temperament. Familiarity food can be provided by companion with friends and family members and has a significant role to insure well-being. Given the comprehensiveness of EEF in ITM which covers all human health-related aspects, we can insure health and well-being among our population by promoting and public educating of these principles.

Bayesian inference of the number of factors in gene-expression analysis: application to human virus challenge studies

Directory of Open Access Journals (Sweden)

Hero Alfred

2010-11-01

Full Text Available Abstract Background Nonparametric Bayesian techniques have been developed recently to extend the sophistication of factor models, allowing one to infer the number of appropriate factors from the observed data. We consider such techniques for sparse factor analysis, with application to gene-expression data from three virus challenge studies. Particular attention is placed on employing the Beta Process (BP, the Indian Buffet Process (IBP, and related sparseness-promoting techniques to infer a proper number of factors. The posterior density function on the model parameters is computed using Gibbs sampling and variational Bayesian (VB analysis. Results Time-evolving gene-expression data are considered for respiratory syncytial virus (RSV, Rhino virus, and influenza, using blood samples from healthy human subjects. These data were acquired in three challenge studies, each executed after receiving institutional review board (IRB approval from Duke University. Comparisons are made between several alternative means of per-forming nonparametric factor analysis on these data, with comparisons as well to sparse-PCA and Penalized Matrix Decomposition (PMD, closely related non-Bayesian approaches. Conclusions Applying the Beta Process to the factor scores, or to the singular values of a pseudo-SVD construction, the proposed algorithms infer the number of factors in gene-expression data. For real data the "true" number of factors is unknown; in our simulations we consider a range of noise variances, and the proposed Bayesian models inferred the number of factors accurately relative to other methods in the literature, such as sparse-PCA and PMD. We have also identified a "pan-viral" factor of importance for each of the three viruses considered in this study. We have identified a set of genes associated with this pan-viral factor, of interest for early detection of such viruses based upon the host response, as quantified via gene-expression data.

Bayesian inference of the number of factors in gene-expression analysis: application to human virus challenge studies.

Science.gov (United States)

Chen, Bo; Chen, Minhua; Paisley, John; Zaas, Aimee; Woods, Christopher; Ginsburg, Geoffrey S; Hero, Alfred; Lucas, Joseph; Dunson, David; Carin, Lawrence

2010-11-09

Nonparametric Bayesian techniques have been developed recently to extend the sophistication of factor models, allowing one to infer the number of appropriate factors from the observed data. We consider such techniques for sparse factor analysis, with application to gene-expression data from three virus challenge studies. Particular attention is placed on employing the Beta Process (BP), the Indian Buffet Process (IBP), and related sparseness-promoting techniques to infer a proper number of factors. The posterior density function on the model parameters is computed using Gibbs sampling and variational Bayesian (VB) analysis. Time-evolving gene-expression data are considered for respiratory syncytial virus (RSV), Rhino virus, and influenza, using blood samples from healthy human subjects. These data were acquired in three challenge studies, each executed after receiving institutional review board (IRB) approval from Duke University. Comparisons are made between several alternative means of per-forming nonparametric factor analysis on these data, with comparisons as well to sparse-PCA and Penalized Matrix Decomposition (PMD), closely related non-Bayesian approaches. Applying the Beta Process to the factor scores, or to the singular values of a pseudo-SVD construction, the proposed algorithms infer the number of factors in gene-expression data. For real data the "true" number of factors is unknown; in our simulations we consider a range of noise variances, and the proposed Bayesian models inferred the number of factors accurately relative to other methods in the literature, such as sparse-PCA and PMD. We have also identified a "pan-viral" factor of importance for each of the three viruses considered in this study. We have identified a set of genes associated with this pan-viral factor, of interest for early detection of such viruses based upon the host response, as quantified via gene-expression data.

Glucagon dynamics during hypoglycaemia and food-re-challenge following treatment with vildagliptin in insulin-treated patients with type 2 diabetes.

Science.gov (United States)

Farngren, J; Persson, M; Schweizer, A; Foley, J E; Ahrén, B

2014-09-01

To determine the effects of dipeptidyl peptidase-4 (DPP-4) inhibition on glucagon dynamics in patients with insulin-treated type 2 diabetes (T2D). The study was a single-centre, double-blind, randomized, placebo controlled crossover study in patients with T2D, mean age 59 ± 6 (s.d.) years and mean haemoglobin A1c 7.7 ± 0.8%, treated with exogenous insulin with or without oral antihyperglycaemic agents. Patients received vildagliptin (50 mg BID) or placebo as add-on to insulin for 4 weeks in random order with a 4-week washout in-between. On day 28 of the respective treatment, patients were served a standard meal (500 kcal) followed by a hyperinsulinaemic hypoglycaemic clamp (target 2.5 mmol/l) and a subsequent food re-challenge (700 kcal). The completers population (n = 29) was analysed. Glucose levels were lower with vildagliptin than with placebo during the meal [areas under the curve (AUC) 1.23 ± 0.07 vs. 1.46 ± 0.05 mol/l min, P vildagliptin (AUC 1.98 ± 0.15 vs. 2.15 ± 0.17 nmol/l min, P = 0.016). In contrast, the glucagon counter-regulation to the insulin-induced hypoglycaemia was sustained by vildagliptin (6.05 ± 1.20 pmol/l during vildagliptin vs.6.94 ± 1.09 pmol/l during placebo, NS). During the food re-challenge after hypoglycaemia, glucagon levels were, again, significantly lower after vildagliptin (AUC 1.30 ± 0.11 vs. 1.52 ± 0.12 nmol/l min, P vildagliptin compared to placebo during meal, hypoglycaemia and food re-challenge. Vildagliptin action to block GLP-1 and GIP inactivation by DPP-4 improves glucagon dynamics during hypoglycaemia, hyperglycaemia and food re-challenge. © 2014 John Wiley & Sons Ltd.

Physics challenges in the strong interactions

Energy Technology Data Exchange (ETDEWEB)

Ellis, S.D.

1991-01-01

An overview of the challenges to be faced in the area of the strong interactions during the 1990's is presented. As an illustrative example special attention is given to the analysis of jets as studied at hadron colliders.

"Challenger" through the Eyes of Feyerabend.

Science.gov (United States)

Dombrowski, Paul M.

1994-01-01

Studies the philosophy of Paul Feyerabend with regard to the analysis of raw data and the role of prior adherence to theory in such analyses. Uses Feyerabend's philosophy to analyze the space shuttle Challenger disaster in terms of social constructionism. (HB)

Protection of horses from West Nile virus Lineage 2 challenge following immunization with a whole, inactivated WNV lineage 1 vaccine.

Science.gov (United States)

Bowen, Richard A; Bosco-Lauth, Angela; Syvrud, Kevin; Thomas, Anne; Meinert, Todd R; Ludlow, Deborah R; Cook, Corey; Salt, Jeremy; Ons, Ellen

2014-09-22

Over the last years West Nile virus (WNV) lineage 2 has spread from the African to the European continent. This study was conducted to demonstrate efficacy of an inactivated, lineage 1-based, WNV vaccine (Equip WNV) against intrathecal challenge of horses with a recent isolate of lineage 2 WNV. Twenty horses, sero-negative for WNV, were enrolled and were randomly allocated to one of two treatment groups: an unvaccinated control group (T01, n=10) and a group administered with Equip WNV (T02, n=10). Horses were vaccinated at Day 0 and 21 and were challenged at day 42 with WNV lineage 2, Nea Santa/Greece/2010. Personnel performing clinical observations were blinded to treatment allocation. Sixty percent of the controls had to be euthanized after challenge compared to none of the vaccinates. A significantly lower percentage of the vaccinated animals showed clinical disease (two different clinical observations present on the same day) on six different days of study and the percentage of days with clinical disease was significantly lower in the vaccinated group. A total of 80% of the non-vaccinated horses showed viremia while only one vaccinated animal was positive by virus isolation on a single occasion. Vaccinated animals started to develop antibodies against WNV lineage 2 from day 14 (2 weeks after the first vaccination) and at day 42 (the time of onset of immunity) they had all developed a strong antibody response. Histopathology scores for all unvaccinated animals ranged from mild to very severe in each of the tissues examined (cervical spinal cord, medulla and pons), whereas in vaccinated horses 8 of 10 animals had no lesions and 2 had minimal lesions in one tissue. In conclusion, Equip WNV significantly reduced the number of viremic horses, the duration and severity of clinical signs of disease and mortality following challenge with lineage 2 WNV. Copyright © 2014 Elsevier Ltd. All rights reserved.

Analysis of CO2 Separation from Flue Gas, Pipeline Transportation, and Sequestration in Coal

Energy Technology Data Exchange (ETDEWEB)

Eric P. Robertson

2007-09-01

This report was written to satisfy a milestone of the Enhanced Coal Bed Methane Recovery and CO2 Sequestration task of the Big Sky Carbon Sequestration project. The report begins to assess the costs associated with separating the CO2 from flue gas and then injecting it into an unminable coal seam. The technical challenges and costs associated with CO2 separation from flue gas and transportation of the separated CO2 from the point source to an appropriate sequestration target was analyzed. The report includes the selection of a specific coal-fired power plant for the application of CO2 separation technology. An appropriate CO2 separation technology was identified from existing commercial technologies. The report also includes a process design for the chosen technology tailored to the selected power plant that used to obtain accurate costs of separating the CO2 from the flue gas. In addition, an analysis of the costs for compression and transportation of the CO2 from the point-source to an appropriate coal bed sequestration site was included in the report.

Training Staff to Manage Challenging Behaviour

NARCIS (Netherlands)

Oorsouw, W.M.W.J. van; Embregts, P.J.C.M.; Bosman, A.M.T.; Jahoda, A.

2010-01-01

Background - A training package for staff working with clients presenting challenging behaviour was developed to (1) increase their knowledge regarding challenging behaviour, and (2) to improve the quality of physical intervention techniques. The latter aim was intended to reduce staff anxiety about

Effects of augmentative and alternative communication on challenging behavior: a meta-analysis.

Science.gov (United States)

Walker, Virginia L; Snell, Martha E

2013-06-01

The purposes of this review were to (a) use meta-analytic procedures to examine the effectiveness of single-case AAC intervention research on challenging behaviors exhibited by individuals with disabilities, (b) identify study characteristics that moderate intervention effects, and (c) evaluate the quality of research. The authors provided inferential and descriptive analyses of 54 studies representing 111 participants and estimated effect sizes using the Nonoverlap of All Pairs (NAP) method. Overall, AAC interventions were found to be equally effective across a broad spectrum of participants and interventions. AAC interventions were more effective with younger children than with adults. Interventions in which functional behavior assessments (FBA) were used had significantly larger effect sizes than those that did not use FBAs. Further, functional communication training interventions resulted in larger effect sizes than Picture Exchange Communication System interventions. Descriptive analysis revealed that (a) interventions often occurred in atypical environments (e.g., therapy room, experimental room) and with atypical interventionists (e.g., therapists, researchers), and (b) numerous studies did not exhibit quality characteristics of single-case research.

Regulatory challenges affecting ICT development in Ukraine

DEFF Research Database (Denmark)

Kretova, Olga A.

2017-01-01

in the region in terms of network readiness (64th place out of 139 economies) and global competitiveness (79th place out of 140 economies) in 2016. The significance of ICT policies is broadly understood at the highest political level in Ukraine, however their implementation has always posed a challenge....... And the chapter is organized as follows. Section 2.1 is the introduction; section 2.2 presents an overview of policies and regulations influencing ICTs in Ukraine; section 2.3 presents a snapshot of the national ICT sector which is dominated by the mobile market and the Broadband Access Technology market; Section...... 2.4 provides an insight into the various ICT regulations, how it affected the markets and the challenges in implementing these policies; section 2.5 highlights the role , successes and challenges of implementing regulations aimed at implementing e-government; and section 2.7 concludes this chapter...

Complete protection of cats against feline panleukopenia virus challenge by a recombinant canine adenovirus type 2 expressing VP2 from FPV.

Science.gov (United States)

Yang, Songtao; Xia, Xianzhu; Qiao, Jun; Liu, Quan; Chang, Shuang; Xie, Zhijing; Ju, Huiyan; Zou, Xiaohuan; Gao, Yuwei

2008-03-10

Feline panleukopenia virus (FPV) is an important infectious pathogen of all members of the family Felidae. Here, we describe construction of a replication-competent recombinant canine adenovirus type 2 (CAV-2) expressing the VP2 protein of FPV (CAV-2-VP2) by transfection of MDCK cells with recombinant CAV-2 genome carrying a VP2 expression cassette. Ten 3-month-old cats were vaccinated with the recombinant virus with two boosters at 15-day intervals. All cats developed neutralizing antibodies of titers 1:16-1:32 by day 15 post-primary vaccination, increasing to 1:64-1:128 by day 45. Examination for clinical signs and viral presence, and total white blood cell counts in peripheral blood following FPV challenge, showed that all were completely protected. This recombinant virus appears to provide an effective alternative to attenuated and inactivated vaccines in immunizing cats against feline panleukopenia.

SAFETY BASIS DESIGN DEVELOPMENT CHALLENGES IMECE2007-42747

Energy Technology Data Exchange (ETDEWEB)

RYAN GW

2007-09-24

'Designing in Safety' is a desired part of the development of any new potentially hazardous system, process, or facility. It is a required part of nuclear safety activities as specified in the U.S. Department of Energy (DOE) Order 420.B, Facility Safety. This order addresses the design of nuclear related facilities developed under federal regulation IOCFR830, Nuclear Safety Management. IOCFR830 requires that safety basis documentation be provided to identify how nuclear safety is being adequately addressed as a condition for system operation (e.g., the safety basis). To support the development of the safety basis, a safety analysis is performed. Although the concept of developing a design that addresses 'Safety is simple, the execution can be complex and challenging. This paper addresses those complexities and challenges for the design activity of a system to treat sludge, a corrosion product of spent nuclear fuel, at DOE's Hanford Site in Washington State. The system being developed is referred to as the Sludge Treatment Project (STP). This paper describes the portion of the safety analysis that addresses the selection of design basis events using the experience gained from the STP and the development of design requirements for safety features associated with those events. Specifically, the paper describes the safety design process and the application of the process for two types of potential design basis accidents associated with the operation of the system, (1) flashing spray leaks and (2) splash and splatter leaks. Also presented are the technical challenges that are being addressed to develop effective safety features to deal with these design basis accidents.

SAFETY BASIS DESIGN DEVELOPMENT CHALLENGES IMECE2007-42747

International Nuclear Information System (INIS)

RYAN GW

2007-01-01

'Designing in Safety' is a desired part of the development of any new potentially hazardous system, process, or facility. It is a required part of nuclear safety activities as specified in the U.S. Department of Energy (DOE) Order 420.B, Facility Safety. This order addresses the design of nuclear related facilities developed under federal regulation IOCFR830, Nuclear Safety Management. IOCFR830 requires that safety basis documentation be provided to identify how nuclear safety is being adequately addressed as a condition for system operation (e.g., the safety basis). To support the development of the safety basis, a safety analysis is performed. Although the concept of developing a design that addresses 'Safety is simple, the execution can be complex and challenging. This paper addresses those complexities and challenges for the design activity of a system to treat sludge, a corrosion product of spent nuclear fuel, at DOE's Hanford Site in Washington State. The system being developed is referred to as the Sludge Treatment Project (STP). This paper describes the portion of the safety analysis that addresses the selection of design basis events using the experience gained from the STP and the development of design requirements for safety features associated with those events. Specifically, the paper describes the safety design process and the application of the process for two types of potential design basis accidents associated with the operation of the system, (1) flashing spray leaks and (2) splash and splatter leaks. Also presented are the technical challenges that are being addressed to develop effective safety features to deal with these design basis accidents

Modeling and analysis for big data challenges in nano-imaging

NARCIS (Netherlands)

Grollios, T.

2012-01-01

Very large data sets, often being referred as Big Data, that are hard to collect, store, process and visualize using traditional computational methods are bringing challenges in various domains of the technology world. The resent advances in electron microscopy that enable the collection of large

Respiratory challenge MRI: Practical aspects

Directory of Open Access Journals (Sweden)

Fiona C. Moreton

2016-01-01

Full Text Available Respiratory challenge MRI is the modification of arterial oxygen (PaO2 and/or carbon dioxide (PaCO2 concentration to induce a change in cerebral function or metabolism which is then measured by MRI. Alterations in arterial gas concentrations can lead to profound changes in cerebral haemodynamics which can be studied using a variety of MRI sequences. Whilst such experiments may provide a wealth of information, conducting them can be complex and challenging. In this paper we review the rationale for respiratory challenge MRI including the effects of oxygen and carbon dioxide on the cerebral circulation. We also discuss the planning, equipment, monitoring and techniques that have been used to undertake these experiments. We finally propose some recommendations in this evolving area for conducting these experiments to enhance data quality and comparison between techniques.

LHC luminosity upgrade detector challenges

CERN Multimedia

CERN. Geneva; de Roeck, Albert; Bortoletto, Daniela; Wigmans, Richard; Riegler, Werner; Smith, Wesley H

2006-01-01

LHC luminosity upgrade: detector challenges The upgrade of the LHC machine towards higher luminosity (1035 cm -2s-1) has been studied over the last few years. These studies have investigated scenarios to achieve the increase in peak luminosity by an order of magnitude, as well as the physics potential of such an upgrade and the impact of a machine upgrade on the LHC DETECTORS. This series of lectures will cover the following topics: • Physics motivation and machine scenarios for an order of magnitude increase in the LHC peak luminosity (lecture 1) • Detector challenges including overview of ideas for R&D programs by the LHC experiments: tracking and calorimetry, other new detector developments (lectures 2-4) • Electronics, trigger and data acquisition challenges (lecture 5) Note: the much more ambitious LHC energy upgrade will not be covered

Heat-induced accumulation of protein synthesis elongation factor 1A indicates an important role in heat tolerance in potato

Science.gov (United States)

Heat stress substantially reduces crop productivity worldwide, and will become more severe due to global warming. Identification of proteins involved in heat stress response may help develop varieties for heat tolerance. Eukaryotic elongation factor 1A (eEF1A) is a cytosolic, multifunctional protei...

Clinical predictors of challenging atrioventricular node ablation procedure for rate control in patients with atrial fibrillation.

Science.gov (United States)

Polin, Baptiste; Behar, Nathalie; Galand, Vincent; Auffret, Vincent; Behaghel, Albin; Pavin, Dominique; Daubert, Jean-Claude; Mabo, Philippe; Leclercq, Christophe; Martins, Raphael P

2017-10-15

Atrioventricular node (AVN) ablation is usually a simple procedure but may sometimes be challenging. We aimed at identifying pre-procedural clinical predictors of challenging AVN ablation. Patients referred for AVN ablation from 2009 to 2015 were retrospectively included. Baseline clinical data, procedural variables and outcomes of AVN ablation were collected. A "challenging procedure" was defined 1) total radiofrequency delivery to get persistent AVN block≥400s, 2) need for left-sided arterial approach or 3) failure to obtain AVN ablation. 200 patients were included (71±10years). A total of 37 (18.5%) patients had "challenging" procedures (including 9 failures, 4.5%), while 163 (81.5%) had "non-challenging" ablations. In multivariable analysis, male sex (Odds ratio (OR)=4.66, 95% confidence interval (CI): 1.74-12.46), body mass index (BMI, OR=1.08 per 1kg/m 2 , 95%CI 1.01-1.16), operator experience (OR=0.40, 95%CI 0.17-0.94), and moderate-to-severe tricuspid regurgitation (TR, OR=3.65, 95%CI 1.63-8.15) were significant predictors of "challenging" ablations. The proportion as a function of number of predictors was analyzed (from 0 to 4, including male sex, operator inexperience, a BMI>23.5kg/m 2 and moderate-to-severe TR). There was a gradual increase in the risk of "challenging" procedure with the number of predictors by patient (No predictor: 0%; 1 predictor: 6.3%; 2 predictors: 16.5%; 3 predictors: 32.5%; 4 predictors: 77.8%). Operator experience, male sex, higher BMI and the degree of TR were independent predictors of "challenging" AVN ablation procedure. The risk increases with the number of predictors by patient. Copyright © 2017. Published by Elsevier B.V.

Mobility Challenges

DEFF Research Database (Denmark)

Jensen, Ole B.; Lassen, Claus

2011-01-01

This article takes point of departure in the challenges to understand the importance of contemporary mobility. The approach advocated is a cross-disciplinary one drawing on sociology, geography, urban planning and design, and cultural studies. As such the perspective is to be seen as a part...... of the so-called ‘mobility turn’ within social science. The perspective is illustrative for the research efforts at the Centre for Mobility and Urban Studies (C-MUS), Aalborg University. The article presents the contours of a theoretical perspective meeting the challenges to research into contemporary urban...... mobilities. In particular the article discusses 1) the physical city, its infrastructures and technological hardware/software, 2) policies and planning strategies for urban mobility and 3) the lived everyday life in the city and the region....

Understanding doctors' ethical challenges as role virtue conflicts.

Science.gov (United States)

McDougall, Rosalind

2013-01-01

This paper argues that doctors' ethical challenges can be usefully conceptualised as role virtue conflicts. The hospital environment requires doctors to be simultaneously good doctors, good team members, good learners and good employees. I articulate a possible set of role virtues for each of these four roles, as a basis for a virtue ethics approach to analysing doctors' ethical challenges. Using one junior doctor's story, I argue that understanding doctors' ethical challenges as role virtue conflicts enables recognition of important moral considerations that are overlooked by other approaches to ethical analysis. © 2011 Blackwell Publishing Ltd.

Sakhalin 2, phase 2 project : meeting the Arctic challenge in a sub-Arctic environment

International Nuclear Information System (INIS)

Reece, A.; Gerges, A.; Efthymiou, M.; Winkler, M.

2008-01-01

Sakhalin Energy's objective is to commercially develop, operate and market the hydrocarbon resources and associated infrastructure governed by the Sakhalin 2 licenses for the sustainable benefit of shareholders, the Russian Federation, the Sakhalin Oblast and the wider community. This presentation discussed Sakhalin Energy's holdings including its investment company and hydrocarbon projects in eastern Russia. The Sakhalin area is a remote island that lacks infrastructure, has a low population density, with a rich onshore and offshore wildlife. It is a seismically active area, with seasonal operating windows, drifting pack ice from December to June, waves up to 18 metre maximum height, and frequent fog in spring and summer. The temperature also varies from 28 degrees Celsius to -40 degrees Celsius. The presentation also addressed the rerouting of offshore pipelines to ensure the least possible disturbance to western gray whale migration. Several photographs and illustrations of the phase 1 project were presented. Open water conditions were also illustrated. The phase 2 project was then outlined. This included illustrations of the platform, plant and export terminal, and onshore processing facility. Other photographs that were shown in the presentation included the offshore pipeline installation; a view of the shore approach to the pipeline installation; oil spill response; and escape, evacuation, and response. The design challenges and design philosophy of the project were also identified. The presentation concluded with a discussion of ice loading and platform response. figs

Sakhalin 2, phase 2 project : meeting the Arctic challenge in a sub-Arctic environment

Energy Technology Data Exchange (ETDEWEB)

Reece, A.; Gerges, A.; Efthymiou, M.; Winkler, M. [Sakhalin Energy, Moscow (Russian Federation)

2008-09-15

Sakhalin Energy's objective is to commercially develop, operate and market the hydrocarbon resources and associated infrastructure governed by the Sakhalin 2 licenses for the sustainable benefit of shareholders, the Russian Federation, the Sakhalin Oblast and the wider community. This presentation discussed Sakhalin Energy's holdings including its investment company and hydrocarbon projects in eastern Russia. The Sakhalin area is a remote island that lacks infrastructure, has a low population density, with a rich onshore and offshore wildlife. It is a seismically active area, with seasonal operating windows, drifting pack ice from December to June, waves up to 18 metre maximum height, and frequent fog in spring and summer. The temperature also varies from 28 degrees Celsius to -40 degrees Celsius. The presentation also addressed the rerouting of offshore pipelines to ensure the least possible disturbance to western gray whale migration. Several photographs and illustrations of the phase 1 project were presented. Open water conditions were also illustrated. The phase 2 project was then outlined. This included illustrations of the platform, plant and export terminal, and onshore processing facility. Other photographs that were shown in the presentation included the offshore pipeline installation; a view of the shore approach to the pipeline installation; oil spill response; and escape, evacuation, and response. The design challenges and design philosophy of the project were also identified. The presentation concluded with a discussion of ice loading and platform response. figs.

Problem Analysis: Challenging All Learners

Science.gov (United States)

Garcia, Katie; Davis, Alicia

2013-01-01

In this article, Garcia and Davis describe problem analysis as the process of examining a given mathematics exercise to find ways in which the problem can be modified and extended to create a richer learning opportunity for students. Students are often reluctant to attempt what they perceive to be higher-order thinking problems, but problem…

Identification and expression analysis of genes associated with bovine blastocyst formation

Directory of Open Access Journals (Sweden)

Van Zeveren Alex

2007-06-01

Full Text Available Abstract Background Normal preimplantation embryo development encompasses a series of events including first cleavage division, activation of the embryonic genome, compaction and blastocyst formation. First lineage differentiation starts at the blastocyst stage with the formation of the trophectoderm and the inner cell mass. The main objective of this study was the detection, identification and expression analysis of genes associated with blastocyst formation in order to help us better understand this process. This information could lead to improvements of in vitro embryo production procedures. Results A subtractive cDNA library was constructed enriched for transcripts preferentially expressed at the blastocyst stage compared to the 2-cell and 8-cell stage. Sequence information was obtained for 65 randomly selected clones. The RNA expression levels of 12 candidate genes were determined throughout 3 stages of preimplantation embryo development (2-cell, 8-cell and blastocyst and compared with the RNA expression levels of in vivo "golden standard" embryos using real-time PCR. The RNA expression profiles of 9 (75% transcripts (KRT18, FN1, MYL6, ATP1B3, FTH1, HINT1, SLC25A5, ATP6V0B, RPL10 were in agreement with the subtractive cDNA cloning approach, whereas for the remaining 3 (25% (ACTN1, COPE, EEF1A1 the RNA expression level was equal or even higher at the earlier developmental stages compared to the blastocyst stage. Moreover, significant differences in RNA expression levels were observed between in vitro and in vivo produced embryos. By immunofluorescent labelling, the protein expression of KRT18, FN1 and MYL6 was determined throughout bovine preimplantation embryo development and showed the same pattern as the RNA expression analyses. Conclusion By subtractive cDNA cloning, candidate genes involved in blastocyst formation were identified. For several candidate genes, important differences in gene expression were observed between in vivo and in

Differential proteomic analysis of Aspergillus fumigatus morphotypes reveals putative drug targets.

Science.gov (United States)

Kubitschek-Barreira, Paula H; Curty, Nathalia; Neves, Gabriela W P; Gil, Concha; Lopes-Bezerra, Leila M

2013-01-14

Aspergillus fumigatus is the main etiological agent of invasive aspergillosis, an important opportunistic infection for neutropenic patients. The main risk groups are patients with acute leukemia and bone marrow transplantation recipients. The lack of an early diagnostic test together with the limited spectrum of antifungal drugs remains a setback to the successful treatment of this disease. During invasive infection the inhaled fungal conidia enter the morphogenic cycle leading to angioinvasive hyphae. This work aimed to study differentially expressed proteins of A. fumigatus during morphogenesis. To achieve this goal, a 2D-DIGE approach was applied to study surface proteins extractable by reducing agents of two A. fumigatus morphotypes: germlings and hyphae. Sixty-three differentially expressed proteins were identified by MALDI-ToF/MS. We observed that proteins associated with biosynthetic pathways and proteins with multiple functions (miscellaneous) were over-expressed in the early stages of germination, while in hyphae, the most abundant proteins detected were related to metabolic processes or have unknown functions. Among the most interesting proteins regulated during morphogenesis, two putative drug targets were identified, the translational factor, eEF3 and the CipC-like protein. Neither of these proteins are present in mammalian cells. Copyright © 2012 Elsevier B.V. All rights reserved.

Compressor Modeling for Transient Analysis of Supercritical CO2 Brayton Cycle by using MARS code

Energy Technology Data Exchange (ETDEWEB)

Park, Joo Hyun; Park, Hyun Sun; Kim, Tae Ho; Kwon, Jin Gyu [POSTECH, Pohang (Korea, Republic of); Bae, Sung Won; Cha, Jae Eun [KAERI, Daejeon (Korea, Republic of)

2016-05-15

In this study, SCIEL (Supercritical CO{sub 2} Integral Experimental Loop) was chosen as a reference loop and the MARS code was as the transient cycle analysis code. As a result, the compressor homologous curve was developed from the SCIEL experimental data and MARS analysis was performed and presented in the paper. The advantages attract SCO{sub 2}BC as a promising next generation power cycles. The high thermal efficiency comes from the operation of compressor near the critical point where the properties of SCO{sub 2}. The approaches to those of liquid phase, leading drastically lower the compression work loss. However, the advantage requires precise and smooth operation of the cycle near the critical point. However, it is one of the key technical challenges. The experimental data was steady state at compressor rotating speed of 25,000 rpm. The time, 3133 second, was starting point of steady state. Numerical solutions were well matched with the experimental data. The mass flow rate from the MARS analysis of approximately 0.7 kg/s was close to the experimental result of 0.9 kg/s. It is expected that the difference come from the measurement error in the experiment. In this study, the compressor model was developed and implemented in MARS to study the transient analysis of SCO{sub 2}BC in SCIEL. We obtained the homologous curves for the SCIEL compressor using experimental data and performed nodalization of the compressor model using MARS code. In conclusions, it was found that numerical solutions from the MARS model were well matched with experimental data.

76 FR 41526 - Centennial Challenges 2011 Strong Tether Challenge

Science.gov (United States)

2011-07-14

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (11-063)] Centennial Challenges 2011 Strong... scheduled and teams that wish to compete may register. Centennial Challenges is a program of prize... NASA Centennial Challenges Program please visit: http://www.nasa.gov/challenges . General questions and...

78 FR 19742 - Centennial Challenges: 2014 Night Rover Challenge

Science.gov (United States)

2013-04-02

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 13-032] Centennial Challenges: 2014 Night... Centennial Challenges 2014 Night Rover Challenge. SUMMARY: This notice is issued in accordance with 51 U.S.C.... Centennial Challenges is a program of prize competitions to stimulate innovation in technologies of interest...

ITER safety challenges and opportunities

International Nuclear Information System (INIS)

Piet, S.J.

1991-01-01

Results of the Conceptual Design Activity (CDA) for the International Thermonuclear Experimental Reactor (ITER) suggest challenges and opportunities. ''ITER is capable of meeting anticipated regulatory dose limits,'' but proof is difficult because of large radioactive inventories needing stringent radioactivity confinement. We need much research and development (R ampersand D) and design analysis to establish that ITER meets regulatory requirements. We have a further opportunity to do more to prove more of fusion's potential safety and environmental advantages and maximize the amount of ITER technology on the path toward fusion power plants. To fulfill these tasks, we need to overcome three programmatic challenges and three technical challenges. The first programmatic challenge is to fund a comprehensive safety and environmental ITER R ampersand D plan. Second is to strengthen safety and environment work and personnel in the international team. Third is to establish an external consultant group to advise the ITER Joint Team on designing ITER to meet safety requirements for siting by any of the Parties. The first of the three key technical challenges is plasma engineering -- burn control, plasma shutdown, disruptions, tritium burn fraction, and steady state operation. The second is the divertor, including tritium inventory, activation hazards, chemical reactions, and coolant disturbances. The third technical challenge is optimization of design requirements considering safety risk, technical risk, and cost. Some design requirements are now too strict; some are too lax. Fuel cycle design requirements are presently too strict, mandating inappropriate T separation from H and D. Heat sink requirements are presently too lax; they should be strengthened to ensure that maximum loss of coolant accident temperatures drop

Tennessee Eastman Plant-wide Industrial Process Challenge Problem

DEFF Research Database (Denmark)

Sales-Cruz, Mauricio; Cameron, Ian; Gani, Rafiqul

2011-01-01

This chapter presents a comprehensive analysis and modelling of the Tennessee Eastman challenge problem. Both a simplified model of the system as well as a full process model that includes the energy balances is given. In each case a full model analysis is carried out to establish the degrees...

SWATH2stats: An R/Bioconductor Package to Process and Convert Quantitative SWATH-MS Proteomics Data for Downstream Analysis Tools.

Science.gov (United States)

Blattmann, Peter; Heusel, Moritz; Aebersold, Ruedi

2016-01-01

SWATH-MS is an acquisition and analysis technique of targeted proteomics that enables measuring several thousand proteins with high reproducibility and accuracy across many samples. OpenSWATH is popular open-source software for peptide identification and quantification from SWATH-MS data. For downstream statistical and quantitative analysis there exist different tools such as MSstats, mapDIA and aLFQ. However, the transfer of data from OpenSWATH to the downstream statistical tools is currently technically challenging. Here we introduce the R/Bioconductor package SWATH2stats, which allows convenient processing of the data into a format directly readable by the downstream analysis tools. In addition, SWATH2stats allows annotation, analyzing the variation and the reproducibility of the measurements, FDR estimation, and advanced filtering before submitting the processed data to downstream tools. These functionalities are important to quickly analyze the quality of the SWATH-MS data. Hence, SWATH2stats is a new open-source tool that summarizes several practical functionalities for analyzing, processing, and converting SWATH-MS data and thus facilitates the efficient analysis of large-scale SWATH/DIA datasets.

77 FR 70835 - Centennial Challenges 2013 Sample Return Robot Challenge

Science.gov (United States)

2012-11-27

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION Centennial Challenges 2013 Sample Return Robot... Challenge is scheduled and teams that wish to compete may register. Centennial Challenges is a program of... Challenge, please visit: http://challenge.wpi.edu . For general information on the NASA Centennial...

Fastidious Gram-Negatives: Identification by the Vitek 2 Neisseria-Haemophilus Card and by Partial 16S rRNA Gene Sequencing Analysis

DEFF Research Database (Denmark)

Wolff Sönksen, Ute; Christensen, Jens Jørgen; Nielsen, Lisbeth

2010-01-01

Taxonomy and identification of fastidious Gram negatives are evolving and challenging. We compared identifications achieved with the Vitek 2 Neisseria-Haemophilus (NH) card and partial 16S rRNA gene sequence (526 bp stretch) analysis with identifications obtained with extensive phenotypic...... characterization using 100 fastidious Gram negative bacteria. Seventy-five strains represented 21 of the 26 taxa included in the Vitek 2 NH database and 25 strains represented related species not included in the database. Of the 100 strains, 31 were the type strains of the species. Vitek 2 NH identification...

76 FR 56819 - Centennial Challenges 2012 Sample Return Robot Challenge

Science.gov (United States)

2011-09-14

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (11-079)] Centennial Challenges 2012 Sample... Challenge is scheduled and teams that wish to compete may register. Centennial Challenges is a program of... Challenge, please visit: http://wp.wpi.edu/challenge/ . For general information on the NASA Centennial...

BOLD quantified renal pO2 is sensitive to pharmacological challenges in rats.

Science.gov (United States)

Thacker, Jon; Zhang, Jeff L; Franklin, Tammy; Prasad, Pottumarthi

2017-07-01

Blood oxygen level-dependent (BOLD) MRI has been effectively used to monitor changes in renal oxygenation. However, R2* (or T2*) is not specific to blood oxygenation and is dependent on other factors. This study investigates the use of a statistical model that takes these factors into account and maps BOLD MRI measurements to blood pO2. Spin echo and gradient echo images were obtained in six Sprague-Dawley rats and R2 and R2* maps were computed. Measurements were made at baseline, post-nitric oxide synthase inhibitor (L-NAME), and post-furosemide administration. A simulation of each region was performed to map R2' (computed as R2*-R2) to blood pO2. At baseline, blood pO2 in the outer medulla was 30.5 ± 1.2 mmHg and 51.9 ± 5.2 mmHg in the cortex, in agreement with previous invasive studies. Blood pO2 was found to decrease within the outer medulla following L-NAME (P pO2 in the cortex increased following furosemide (P pO2 is sensitive to pharmacological challenges, and baseline pO2 is comparable to literature values. Reporting pO2 instead of R2* could lead to a greater clinical impact of renal BOLD MRI and facilitate the identification of hypoxic regions. Magn Reson Med 78:297-302, 2017. © 2016 International Society for Magnetic Resonance in Medicine. © 2016 International Society for Magnetic Resonance in Medicine.

Binding free energy predictions of farnesoid X receptor (FXR) agonists using a linear interaction energy (LIE) approach with reliability estimation: application to the D3R Grand Challenge 2