Automated Formal Verification for PLC Control Systems

CERN Multimedia

Fernández Adiego, Borja

2014-01-01

Programmable Logic Controllers (PLCs) are widely used devices used in industrial control systems. Ensuring that the PLC software is compliant with its specification is a challenging task. Formal verification has become a recommended practice to ensure the correctness of the safety-critical software. However, these techniques are still not widely applied in industry due to the complexity of building formal models, which represent the system and the formalization of requirement specifications. We propose a general methodology to perform automated model checking of complex properties expressed in temporal logics (e.g. CTL, LTL) on PLC programs. This methodology is based on an Intermediate Model (IM), meant to transform PLC programs written in any of the languages described in the IEC 61131-3 standard (ST, IL, etc.) to different modeling languages of verification tools. This approach has been applied to CERN PLC programs validating the methodology.

Translation of PLC Programs to x86 for Simulation and Verification

CERN Document Server

Sallai, Gyula

2017-01-01

PLC programs are written in special languages, variants of the languages defined in the IEC 61131 standard. These programs cannot be directly executed on personal computers (on x86 architecture). To perform simulation of the PLC program or diagnostics during development, either a real PLC or a PLC simulator has to be used. However, these solutions are often inflexible and they do not provide appropriate performance. By generating x86-representations (semantically equivalent programs which can be executed on PCs, e.g. written in C, C++ or Java) of the PLC programs, some of these challenges could be met. PLCverif is a PLC program verification tool developed at CERN which includes a parser for Siemens PLC programs. In this work, we describe a code generator based on this parser of PLCverif. This work explores the possibilities and challenges of generating programs in widely-used general purpose languages from PLC programs, and provides a proof-of-concept code generation implementation. The presented solution dem...

Maximizing Academic Library Collections: Measuring Changes in Use Patterns Owing to EBSCO Discovery Service

Science.gov (United States)

Calvert, Kristin

2015-01-01

Despite the prevalence of academic libraries adopting web-scale discovery tools, few studies have quantified their effect on the use of library collections. This study measures the impact that EBSCO Discovery Service has had on use of library resources through circulation statistics, use of electronic resources, and interlibrary loan requests.…

A Classification of PLC Models and Applications

NARCIS (Netherlands)

Mader, Angelika H.; Boel, R.; Stremersch, G.

In the past years there is an increasing interest in analysing PLC applications with formal methods. The first step to this end is to get formal models of PLC applications. Meanwhile, various models for PLCs have already been introduced in the literature. In our paper we discuss several

A Tale of Two Discoveries: Comparing the Usability of Summon and EBSCO Discovery Service

Science.gov (United States)

Foster, Anita K.; MacDonald, Jean B.

2013-01-01

Web-scale discovery systems are gaining momentum among academic libraries as libraries seek a means to provide their users with a one-stop searching experience. Illinois State University's Milner Library found itself in the unique position of having access to two distinct discovery products, EBSCO Discovery Service and Serials Solutions' Summon.…

PLC control of 50 MW klystron modulators

International Nuclear Information System (INIS)

Shang Lei; Liu Gongfa; Chen Liping; Lu Yeming; Hong Jun; Zhang Yi; Zhao Feng

2004-01-01

Upgrade project of the 50 MW klystron modulators of Hefei Light Source (HLS) was firstly introduced. PLC control system of modulators was employed to replace the old control and monitor system, which was based on relay logic circuit and manual operation method. the PLC system becomes a sub system of the new EPICS control system of HLS. Constant-current, switch-mode and high voltage power supplies were adopted to replace the old 50 Hz power supplies. The technology of modulators was improved and operation was more reliable. The design method, hardware and software of PLC control of modulators were described and the performance was presented. (authors)

The Integration of DCS I/O to an Existing PLC

Science.gov (United States)

Sadhukhan, Debashis; Mihevic, John

2013-01-01

At the NASA Glenn Research Center (GRC), Existing Programmable Logic Controller (PLC) I/O was replaced with Distributed Control System (DCS) I/O, while keeping the existing PLC sequence Logic. The reason for integration of the PLC logic and DCS I/O, along with the evaluation of the resulting system is the subject of this paper. The pros and cons of the old system and new upgrade are described, including operator workstation screen update times. Detail of the physical layout and the communication between the PLC, the DCS I/O and the operator workstations are illustrated. The complex characteristics of a central process control system and the plan to remove the PLC processors in future upgrades is also discussed.

Realizace úzkopásmových PLC modemů

OpenAIRE

Kubíček, Lukáš

2012-01-01

Diplomová práce se věnuje úzkopásmové technologii přenosu dat el. sítí NN (PLC) a~možnostem jejího využití v senzorových sítích. Zabývá se jak strukturou a základními vlastnostmi PLC standardu, tak možnostmi jeho uplatnění v praxi. Hlavní částí této práce je realizace PLC sítě pomocí IO ST7570 firmy STMicroelectronics. Jako příklad praktického využití PLC technologie je tato síť připojena k počítači, ve kterém jsou data ze senzoru (wattmetru) připojenému k PLC modemu ukládána pomocí aplikace ...

78 FR 11976 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-02-21

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... (AD) for all Rolls-Royce plc (RR) RB211-524 series turbofan engines. That AD currently requires...-16724 (76 FR 40217, July 8, 2011), for all RR plc RB211-524 series turbofan engines. That AD required...

77 FR 6668 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2012-02-09

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... all Rolls-Royce plc RB211-Trent 500 series turbofan engines. This AD requires a one-time inspection of... RB211- Trent 560A2-61 turbofan engines that have not complied with Rolls- Royce plc Service Bulletin No...

PLC based control system for RAM assembly test facility

International Nuclear Information System (INIS)

Kulkarni, S.S.; Kumar, Vinaya; Chandra, Umesh

1994-01-01

The flexibility, expandability, ease of programming and diagnostic features makes the programmable logic controller (PLC) suitable for a variety of control applications in engineering system test facilities. A PLC based control system for RAM assembly test facility (RATF) and for testing the related hydraulic components is being developed and installed at BARC. This paper describes the approach taken for meeting the control requirements and illustrates the PLC software that has been developed. (author). 1 fig

The application of PLC in 60Co container inspection system

International Nuclear Information System (INIS)

Huang Yibin; Xiang Xincheng

2001-01-01

The author discusses the interlock technique of 60 Co container inspection system, and introduces the hardware structure and program of interlock control system using PLC. Due to adopting PLC distributed control, the system works stably and reliably. The successful application of PLC in 60 Co container inspection system has some use for reference in nuclear technology field

Parameters Evaluation of PLC Dependability and Safety

Directory of Open Access Journals (Sweden)

Juraj Zdansky

2006-01-01

Full Text Available This paper is focused on evaluation of dependability and safety parameters of PLC (Programmable Logic Controller. Achievement of requested level of these parameters is an application assumption for using PLC in control of safety critical processes. Evaluation of these parameters can be made on the base of suitable model and it can be influenced by system architecture when necessary.

Automated turn pike using PLC and SCADA

Science.gov (United States)

Silpa Sreedhar, P.; Aiswarya, P.; Kathirvelan, J.

2017-11-01

We propose a smart turnpike based on Programmable Logic Controller (PLC) and Supervisory Control and Data Acquisition Systems (SCADA) in this paper. In this work, the basic idea is to measure the weight of the vehicles and classify them according to its weight to the respective lanes. It is difficult for the turnpike people to monitor the whole process all the time. So, this PLC based diversion system can be implemented in turnpikes to reduce the difficulties. This method will work based on weight sensors (piezo-resistive) whose output will be fed to a PLC, which will control the vehicle diversion. Using SCADA software, the whole process can be monitored from a remote area. The algorithm developed in this successfully installed in real time system.

Power Line Communication (PLC) in Space - Current Status and Outlook

Science.gov (United States)

Wolf, J.

2012-05-01

The Power Line Communication (PLC) technology as known from various terrestrial applications, e.g. in building automation, in the automotive sector and on aircraft, appears to be a promising technology for the use on spacecraft. Starting from a critical overview on existing terrestrial PLC applications with their pros and cons, the paper gives a motivation for the introduction of the PLC technology on spacecraft, discusses the potential areas where it can be applied and is highlighting the potential problem areas. A short overview of on-going ESA PLC activities is provided and an outlook is given.

PLC-based mode multi/demultiplexers for mode division multiplexing

Science.gov (United States)

Saitoh, Kunimasa; Hanzawa, Nobutomo; Sakamoto, Taiji; Fujisawa, Takeshi; Yamashita, Yoko; Matsui, Takashi; Tsujikawa, Kyozo; Nakajima, Kazuhide

2017-02-01

Recently developed PLC-based mode multi/demultiplexers (MUX/DEMUXs) for mode division multiplexing (MDM) transmission are reviewed. We firstly show the operation principle and basic characteristics of PLC-based MUX/DEMUXs with an asymmetric directional coupler (ADC). We then demonstrate the 3-mode (2LP-mode) multiplexing of the LP01, LP11a, and LP11b modes by using fabricated PLC-based mode MUX/DEMUX on one chip. In order to excite LP11b mode in the same plane, a PLC-based LP11 mode rotator is introduced. Finally, we show the PLC-based 6-mode (4LP-mode) MUX/DEMUX with a uniform height by using ADCs, LP11 mode rotators, and tapered waveguides. It is shown that the LP21a mode can be excited from the LP11b mode by using ADC, and the two nearly degenerated LP21b and LP02 modes can be (de)multiplexed separately by using tapered mode converter from E13 (E31) mode to LP21b (LP02) mode.

Applying Model Checking to Industrial-Sized PLC Programs

CERN Document Server

AUTHOR|(CDS)2079190; Darvas, Daniel; Blanco Vinuela, Enrique; Tournier, Jean-Charles; Bliudze, Simon; Blech, Jan Olaf; Gonzalez Suarez, Victor M

2015-01-01

Programmable logic controllers (PLCs) are embedded computers widely used in industrial control systems. Ensuring that a PLC software complies with its specification is a challenging task. Formal verification has become a recommended practice to ensure the correctness of safety-critical software but is still underused in industry due to the complexity of building and managing formal models of real applications. In this paper, we propose a general methodology to perform automated model checking of complex properties expressed in temporal logics (\\eg CTL, LTL) on PLC programs. This methodology is based on an intermediate model (IM), meant to transform PLC programs written in various standard languages (ST, SFC, etc.) to different modeling languages of verification tools. We present the syntax and semantics of the IM and the transformation rules of the ST and SFC languages to the nuXmv model checker passing through the intermediate model. Finally, two real cases studies of \\CERN PLC programs, written mainly in th...

Automatic control model of water filling system with Allen Bradley Micrologix 1400 PLC

Science.gov (United States)

Harahap, R.; Adyatma, AF; Fahmi, F.

2018-02-01

Programmable Logic Controller or PLC today plays an important role in most industrial control systems. PLC usage can be encountered in almost all fields of industry, not only in the manufacturing world but also on many other things such as elevators in office buildings, hotels hospitals, and others. PLC is an electronic control tool that operates in logic that its programming can be modified with relative ease. As with any controller in general, the PLC processes input signals to further discharge output according to the desired program. PLC usage is very broad because of its high reliability, can be reprogrammed or modified with relative ease, and very helpful in the tracking troubleshooting. One type of existing PLC is Allen Bradley PLC. Allen Bradley PLC program is commonly used in various industries. PLC Allen Bradley (AB) has several types, and one of them is the type of Micrologic 1400. In this study we design a system as a comparison with the conventional system. For that to explore the use of a PLC program which will be supported by a simulator tool, including a program to RSLogic 500, how to programming, monitoring via RSView32, and modification. It is expected to understand the application aspect of operation and programming of this specific PLC and its potential. The purpose of this research is to design water filling automation system by using Allen Bradley Micrologic 1400 type 1766-L32BXB PLC, empowering the use of Allen Bradley Micrologic 1400 PLC and to regulate the desired process to obtain efficiency and effectiveness compared with conventional system arrangement using Relay.

Transacsys PLC - Final Results

CERN Multimedia

2002-01-01

Final results from Transacsys PLC. A subsidary of this company was set up to develop the CERN EDH system into a commercial product but incurred too much financial loss so the project was cancelled (1/2 page).

Web-scale discovery in an academic health sciences library: development and implementation of the EBSCO Discovery Service.

Science.gov (United States)

Thompson, Jolinda L; Obrig, Kathe S; Abate, Laura E

2013-01-01

Funds made available at the close of the 2010-11 fiscal year allowed purchase of the EBSCO Discovery Service (EDS) for a year-long trial. The appeal of this web-scale discovery product that offers a Google-like interface to library resources was counter-balanced by concerns about quality of search results in an academic health science setting and the challenge of configuring an interface that serves the needs of a diverse group of library users. After initial configuration, usability testing with library users revealed the need for further work before general release. Of greatest concern were continuing issues with the relevance of items retrieved, appropriateness of system-supplied facet terms, and user difficulties with navigating the interface. EBSCO has worked with the library to better understand and identify problems and solutions. External roll-out to users occurred in June 2012.

Electromagnetic compatibility of PLC adapters for in-home/domestic networks

Science.gov (United States)

Potisk, Lukas; Hallon, Jozef; Orgon, Milos; Fujdiak, Radek

2018-01-01

The use of programable logic controllers (PLC) technology in electrical networks 230 V causes electromagnetic radiation that interferes with other electrical equipment connected to the network [1-4]. Therefore, this article describes the issues of electromagnetic compatibility (EMC) of new PLC adapters used in IP broadband services in a multi-user environment. The measurements of disturbing electromagnetic field originated in PLC adapters were made in a certified laboratory EMC (laboratory of electromagnetic compatibility) in the Institute of Electrical Engineering at Faculty of Electrical Engineering and Information Technology of the Slovak University of Technology in Bratislava. The measured spectra of the radiated electromagnetic field will be compared with the results obtained when testing older PLC modems [5].

Optical interconnection for a polymeric PLC device using simple positional alignment.

Science.gov (United States)

Ryu, Jin Hwa; Kim, Po Jin; Cho, Cheon Soo; Lee, El-Hang; Kim, Chang-Seok; Jeong, Myung Yung

2011-04-25

This study proposes a simple cost-effective method of optical interconnection between a planar lightwave circuit (PLC) device chip and an optical fiber. It was conducted to minimize and overcome the coupling loss caused by lateral offset which is due to the process tolerance and the dimensional limitation existing between PLC device chips and fiber array blocks with groove structures. A PLC device chip and a fiber array block were simultaneously fabricated in a series of polymer replication processes using the original master. The dimensions (i.e., width and thickness) of the under-clad of the PLC device chip were identical to those of the fiber array block. The PLC device chip and optical fiber were aligned by simple positional control for the vertical direction of the PLC device chip under a particular condition. The insertion loss of the proposed 1 x 2 multimode optical splitter device interconnection was 4.0 dB at 850 nm and the coupling loss was below 0.1 dB compared with single-fiber based active alignment.

Practical application with plc in manipulation of a robotic arm

Directory of Open Access Journals (Sweden)

Cristian Barz

2014-12-01

Full Text Available This paper presents the use of a robotic arm PLC Siemens in order not using CNC commands. This is done by programming the PLC ladder diagram language that makes movement on the three axes of the arm by means of stepper motors. Required command console PLC is built with the help of a touch screen HMI Weintek. In the user interface are introduced distances and displacement speeds on the three axes.

Reliability Analysis for Safety Grade PLC(POSAFE-Q)

International Nuclear Information System (INIS)

Choi, Kyung Chul; Song, Seung Whan; Park, Gang Min; Hwang, Sung Jae

2012-01-01

Safety Grade PLC(Programmable Logic Controller), POSAFE-Q, was developed recently in accordance with nuclear regulatory and requirements. In this paper, describe reliability analysis for digital safety grade PLC (especially POSAFE-Q). Reliability analysis scope is Prediction, Calculation of MTBF (Mean Time Between Failure), FMEA (Failure Mode Effect Analysis), PFD (Probability of Failure on Demand). (author)

Full-automatic Special Drill Hydraulic System and PLC Control

Directory of Open Access Journals (Sweden)

Tian Xue Jun

2016-01-01

Full Text Available A hydraulic-driven and PLC full-automatic special drill is introduced, working principle of the hydraulic system and PLC control system are analyzed and designed, this equipment has the advantages of high efficiency, superior quality and low cost etc.

Efficient Implementation of a Symbol Timing Estimator for Broadband PLC.

Science.gov (United States)

Nombela, Francisco; García, Enrique; Mateos, Raúl; Hernández, Álvaro

2015-08-21

Broadband Power Line Communications (PLC) have taken advantage of the research advances in multi-carrier modulations to mitigate frequency selective fading, and their adoption opens up a myriad of applications in the field of sensory and automation systems, multimedia connectivity or smart spaces. Nonetheless, the use of these multi-carrier modulations, such as Wavelet-OFDM, requires a highly accurate symbol timing estimation for reliably recovering of transmitted data. Furthermore, the PLC channel presents some particularities that prevent the direct use of previous synchronization algorithms proposed in wireless communication systems. Therefore more research effort should be involved in the design and implementation of novel and robust synchronization algorithms for PLC, thus enabling real-time synchronization. This paper proposes a symbol timing estimator for broadband PLC based on cross-correlation with multilevel complementary sequences or Zadoff-Chu sequences and its efficient implementation in a FPGA; the obtained results show a 90% of success rate in symbol timing estimation for a certain PLC channel model and a reduced resource consumption for its implementation in a Xilinx Kyntex FPGA.

Efficient Implementation of a Symbol Timing Estimator for Broadband PLC

Directory of Open Access Journals (Sweden)

Francisco Nombela

2015-08-01

Full Text Available Broadband Power Line Communications (PLC have taken advantage of the research advances in multi-carrier modulations to mitigate frequency selective fading, and their adoption opens up a myriad of applications in the field of sensory and automation systems, multimedia connectivity or smart spaces. Nonetheless, the use of these multi-carrier modulations, such as Wavelet-OFDM, requires a highly accurate symbol timing estimation for reliably recovering of transmitted data. Furthermore, the PLC channel presents some particularities that prevent the direct use of previous synchronization algorithms proposed in wireless communication systems. Therefore more research effort should be involved in the design and implementation of novel and robust synchronization algorithms for PLC, thus enabling real-time synchronization. This paper proposes a symbol timing estimator for broadband PLC based on cross-correlation with multilevel complementary sequences or Zadoff-Chu sequences and its efficient implementation in a FPGA; the obtained results show a 90% of success rate in symbol timing estimation for a certain PLC channel model and a reduced resource consumption for its implementation in a Xilinx Kyntex FPGA.

Silica-based PLC with heterogeneously-integrated PDs for one-chip DP-QPSK receiver.

Science.gov (United States)

Kurata, Yu; Nasu, Yusuke; Tamura, Munehisa; Kasahara, Ryoichi; Aozasa, Shinichi; Mizuno, Takayuki; Yokoyama, Haruki; Tsunashima, Satoshi; Muramoto, Yoshifumi

2012-12-10

To realize a DP-QPSK receiver PLC, we heterogeneously integrated eight high-speed PDs on a silica-based PLC platform with a PBS, 90-degree optical hybrids and a VOA. The use of a 2.5%-Δ waveguide reduced the receiver PLC size to 11 mm x 11 mm. We successfully demonstrated 32 Gbaud DP-QPSK signal demodulation with the receiver PLC.

Control Systems of Rubber Dryer Machinery Components Using Programmable Logic Control (PLC)

Science.gov (United States)

Hendra; Yulianto, A. S.; Indriani, A.; Hernadewita; Hermiyetti

2018-02-01

Application of programmable logic control (PLC) is widely used on the control systems in the many field engineering such as automotive, aviation, food processing and other industries [1-2]. PLC is simply program to control many automatic activity, easy to use, flexible and others. PLC using the ladder program to solve and regulated the control system component. In previous research, PLC was used for control system of rotary dryer machine. In this paper PLC are used for control system of motion component in the rubber dryer machinery. Component of rubber dryer machine is motors, gearbox, sprocket, heater, drying chamber and bearing. Principle working of rubber dryer machinery is wet rubber moving into the drying chamber by sprocket. Sprocket is driven by motors that conducted by PLC to moving and set of wet rubber on the drying chamber. Drying system uses greenhouse effect by making hanger dryer design in the form of line path. In this paper focused on motion control system motors and sensors drying rubber using PLC. The results show that control system of rubber dryer machinery can work in accordance control input and the time required to dry the rubber.

Development of Multipurpose PLC trainer for the simulator of reactor safety system

International Nuclear Information System (INIS)

Syaiful Bakhri; Deswandri; Ahmad Abtokhi

2014-01-01

PLC becomes one of the essential components for the current type of reactor which based on digital instrumentation and control. Several studies have demonstrated the promising results including the implementation of PLC's for RSG-GAS research reactor. However, research for the safety and reliability analysis can not be carried out freely in the existing systems.Therefore, this research aims to develop a PLC trainer employing micro PLC OMRON CP1MA which can be useful for simulator of various topics in reactor safety. Two experimental tests were carried out to show the PLC’s performances. The first experimental testing implementing reactor protection system of research reactor RSG-GAS shows the capacity of PLC system to identify the initiator of the SCRAM logic as well as giving a promptly response. Secondly, the application of PLC to controls the water level in dual reservoir system simulation, demonstrates the simplicity of the operation and design while maintaining the best performances. (author)

A study of PLC system vulnerability checklists in nuclear power plants

International Nuclear Information System (INIS)

Cha, Ki Jong; Cho, Gi Ho; Ahn, Jaeh Young; Kim, Young Mi; Kwon, Yong Il

2012-01-01

Because the design of the PLCs (Programmable Logic Controller) in the I and C (Instrument and Control) systems for NPP (Nuclear Power Plant) were carried out independently, the problems of cyber security were not addressed in the PLC system designs. Recently, the analysis and the countermeasure development for the PLC systems became mandatory due to the developments in cyber attack techniques and the increasingly revealed vulnerability to such attacks. A comparative analysis on the cyber security checklist of PLC in industry control system and in NPP systems was carried out, and in this paper, the cyber security regulatory trend and the PLC usage status are described

A study of PLC system vulnerability checklists in nuclear power plants

Energy Technology Data Exchange (ETDEWEB)

Cha, Ki Jong; Cho, Gi Ho; Ahn, Jaeh Young [Convergence technology Research Commercialization Center, Daejeon (Korea, Republic of); Kim, Young Mi; Kwon, Yong Il [Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of)

2012-10-15

Because the design of the PLCs (Programmable Logic Controller) in the I and C (Instrument and Control) systems for NPP (Nuclear Power Plant) were carried out independently, the problems of cyber security were not addressed in the PLC system designs. Recently, the analysis and the countermeasure development for the PLC systems became mandatory due to the developments in cyber attack techniques and the increasingly revealed vulnerability to such attacks. A comparative analysis on the cyber security checklist of PLC in industry control system and in NPP systems was carried out, and in this paper, the cyber security regulatory trend and the PLC usage status are described.

Using of Finite Automation at Programming PLC

Directory of Open Access Journals (Sweden)

Karol Rastocny

2004-01-01

Full Text Available The paper is concerning with systematic advances at programming programmable logic controllers (PLC, which comes out from algebraic description of behaviour of sequential circuit, in the way of finite automaton. This kind of access is streamlining the work of a programmer and enabling to use formalisms in the of whole process of system development, that is from process of analysing demands to process of verification and validation created program. The paper considers about using of ladder diagram at programming PLC.

Webová aplikace v PLC PFC200

OpenAIRE

Michalík, David

2017-01-01

Tato bakalářská práce se věnuje implementaci vlastního způsobu ovládání vstupů, výstupů a paměti PLC PFC200 od firmy WAGO pomocí dynamického webového rozhraní. Součástí práce je popis PLC, způsobů jeho ovládání, rešerše volně dostupných webových serverů pro Linux a popis metod pro vytvoření real-time ovládání. Výstupem je webová aplikace běžící na webovém serveru v PLC, využívající vlastní runtime systém formou daemon programů. This bachelor’s thesis is mainly focused on implementation of ...

Справка: Поиск через EBSCO Discovery Service

OpenAIRE

2011-01-01

Представлена краткая справка для просмотра данных каталога при работе с сервисом EBSCO Discovery Service (EDS) на платформе EBSCOhost от компании EBSCO Publishing на русском языке.

Programmable logic controller (PLC) for safety systems of nuclear plants

International Nuclear Information System (INIS)

Sen, S.K.; Karmakar, G.; Joseph, Jose; Patil, R.K.

2002-01-01

Full text: A programmable logic controller (PLC) has been developed by RCnD, BARC for use in the safety critical systems in nuclear power plants. This PLC uses qualified hardware developed in RCnD for use in NPP. The programming software conforms to IEC-61131 part 3. The application programming is done on function block diagram (FBD) editor and the FBD is automatically converted into code in high level language (C / C++). This feature makes the application easily decipherable and therefore easily subjected to reviews and other validation techniques. The key to make quality software for use in nuclear systems is to enforce various standards in the design and development of the software, something, which is not possible to do with a commercially available PLC. This PLC with its software completely transparent lends itself to rigorous verification and validation easily

Trends in Control Area of PLC Reliability and Safety Parameters

Directory of Open Access Journals (Sweden)

Juraj Zdansky

2008-01-01

Full Text Available Extension of the PLC application possibilities is closely related to increase of reliability and safety parameters. If the requirement of reliability and safety parameters will be suitable, the PLC could by implemented to specific applications such the safety-related processes control. The goal of this article is to show the way which producers are approaching to increase PLC`s reliability and safety parameters. The second goal is to analyze these parameters for range of present choice and describe the possibility how the reliability and safety parameters can be affected.

Analysis of the PLC object model in the PF linac

International Nuclear Information System (INIS)

Abe, Isamu; Shirakawa, Akihiro; Nakahara, Kazuo; Tanaka, Masahiko

1995-01-01

Device controllers (front end) for the KEK PF Linac, which has been operated for more than ten years, had to be renewed, because there is no longer support from hardware manufacturer. To reduce maintenance costs, device controllers are planed to be replaced by PLC (Programmable Logic Controller). Investigations launched in the late 1994s for the PLC have carried out in order to make sure of the I/O stability and network possibilities. In this paper, the software for the PLC is discussed based on the OOP concept. (author)

Development of radioactive source scanner based on PLC

International Nuclear Information System (INIS)

Yang Guogui; Gao Xiang; Guo Hongli

2013-01-01

The radioactive radial uniformity of 68 Ge line radioactive sources is a critical quality parameter. The radioactive source scanner with linear scanning function is developed by making use of high-speed pulse counters, high-speed pulse output ports, and the powerful instruction system of Siemens S7-200 series programmable logic controller (PLC). A computer used as a host computer of the instrument communicate with. the PLC by point to point interface (PPI) protocol, The instrument with functions of data collection, transmission, displaying, saving, motion control and instrument parameter settings, can be used to measure the radioactive radial uniformity and total activity of line radioactive source. The advantages of Using the PLC to develop nuclear instrumentation are development speed, strong anti-interference ability, and low-cost. This paper mainly describes the control system implementation and feature of the instrument. (authors)

Úzkopásmová PLC komunikace pro Smart Metering

OpenAIRE

Kubů, Jiří

2016-01-01

Tato bakalářská práce se zabývá úzkopásmovou PLC komunikací. V práci je popsán základní princip, rozdělení PLC komunikace, historie technologie, využívané komunikační standardy a rušení ovlivňující datovou komunikaci této technologie. V rámci této práce byly provedeny i experimentální měření v reálném prostředí s modemy od firmy Texas Instruments C2000 PLC TMDSPLCKITV4 a C2000 PLC TMDSPLCKIT-V3. Výsledky tohoto měření jsou reálné přenosové rychlosti. Dále byla vytvořena aplikace pro dálkový s...

Construction and Verification of PLC LD-programs by LTL-specification

Directory of Open Access Journals (Sweden)

E. V. Kuzmin

2013-01-01

Full Text Available An approach to construction and verification of PLC LD-programs for discrete problems is proposed. For the specification of the program behavior, we use the linear-time temporal logic LTL. Programming is carried out in the LD-language (Ladder Diagram according to an LTL-specification. The correctness analysis of an LTL-specification is carried out by the symbolic model checking tool Cadence SMV. A new approach to programming and verification of PLC LD-programs is shown by an example. For a discrete problem, we give a LD-program, its LTL-specification and an SMV-model. The purpose of the article is to describe an approach to programming PLC, which would provide a possibility of LD-program correctness analysis by the model checking method. Under the proposed approach, the change of the value of each program variable is described by a pair of LTL-formulas. The first LTL-formula describes situations which increase the value of the corresponding variable, the second LTL-formula specifies conditions leading to a decrease of the variable value. The LTL-formulas (used for speci- fication of the corresponding variable behavior are constructive in the sense that they construct the PLC-program (LD-program, which satisfies temporal properties expressed by these formulas. Thus, the programming of PLC is reduced to the construction of LTLspecification of the behavior of each program variable. In addition, an SMV-model of a PLC LD-program is constructed according to LTL-specification. Then, the SMV-model is analysed by the symbolic model checking tool Cadence SMV.

PLC/DTAM Software Programs for Pumping Instrumentation and Control Skid X

International Nuclear Information System (INIS)

HORNER, T.M.

2001-01-01

This document describes the software programs for the Allen-Bradley SLC 500 programmable logic controller (PLC) and the Allen-Bradley DTAM PLUS operator interface module used on Pumping Instrumentation and Control (PIC) skid ''X''. The software programs for the SLC 500 and DTAM Plus are based on the core programs provided by Allen-Bradley. The PLC and DTAM software programs on skid ''D'' for SX-104 are the baseline programs. These baselines will be tailored for each individual BY-farm skid. An Acceptance Test Procedure (ATP) and an Operational Test Procedure (OTP) verify that the software programs meet the specific requirements for BY-105 pumping. This document represents the final PLC and DTAM programs for PIC skid ''X'' at BY-105. These programs were printed out after the performance of the OTP. The OTP acts as the final qualification test for the software programs. Functional requirements and details of the PLC ladder logic are described in this document. The final programs entered into the PLC and DTAM Plus are included as Appendices to this document

77 FR 13485 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2012-03-07

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... series turbofan engines. This AD requires inspecting the front combustion liner head section for cracking.... (c) Applicability This AD applies to Rolls-Royce plc (RR) RB211-Trent 800 turbofan engines, all...

Modelling and Formal Verification of Timing Aspects in Large PLC Programs

CERN Document Server

Fernandez Adiego, B; Blanco Vinuela, E; Tournier, J-C; Gonzalez Suarez, V M; Blech, J O

2014-01-01

One of the main obstacle that prevents model checking from being widely used in industrial control systems is the complexity of building formal models out of PLC programs, especially when timing aspects need to be integrated. This paper brings an answer to this obstacle by proposing a methodology to model and verify timing aspects of PLC programs. Two approaches are proposed to allow the users to balance the trade-off between the complexity of the model, i.e. its number of states, and the set of specifications possible to be verified. A tool supporting the methodology which allows to produce models for different model checkers directly from PLC programs has been developed. Verification of timing aspects for real-life PLC programs are presented in this paper using NuSMV.

Paths of Discovery: Comparing the Search Effectiveness of EBSCO Discovery Service, Summon, Google Scholar, and Conventional Library Resources

Science.gov (United States)

Asher, Andrew D.; Duke, Lynda M.; Wilson, Suzanne

2013-01-01

In 2011, researchers at Bucknell University and Illinois Wesleyan University compared the search efficacy of Serial Solutions Summon, EBSCO Discovery Service, Google Scholar, and conventional library databases. Using a mixed-methods approach, qualitative and quantitative data were gathered on students' usage of these tools. Regardless of the…

77 FR 73268 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2012-12-10

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... certain Rolls-Royce plc (RR) RB211-Trent 900 series turbofan engines. This AD requires inspection of the... turbofan engines, all serial numbers. (d) Reason This AD was prompted by a Trent 900 engine experiencing a...

77 FR 56760 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2012-09-14

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... all Rolls-Royce plc (RR) RB211-Trent 800 series turbofan engines. This AD requires removing from...-17, 892-17, 892B-17, and 895-17 turbofan engines that have an intermediate pressure (IP) turbine disc...

Emulation of Narrowband Powerline Data Transmission Channels and Evaluation of PLC Systems

OpenAIRE

Liu, Wenqing

2013-01-01

This work proposes advanced emulation of the physical layer behavior of NB-PLC channels and the application of a channel emulator for the evaluation of NB-PLC systems. In addition, test procedures and reference channels are proposed to improve efficiency and accuracy in the system evaluation and classification. This work shows that the channel emulator-based solution opens new ways toward flexible, reliable and technology-independent performance assessment of PLC modems.

78 FR 17297 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-03-21

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... (AD) for all Rolls-Royce plc (RR) RB211 Trent 500 series turbofan engines. That AD currently requires... 9, 2012), for all RR RB211 Trent 500 series turbofan engines. That AD requires a one-time inspection...

Silencing of the tomato phosphatidylinositol-phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea

NARCIS (Netherlands)

Gonorazky, Gabriela; Guzzo, María Carla; Abd-El-Haliem, Ahmed M.; Joosten, Matthieu H.A.J.; Laxalt, Ana María

2016-01-01

The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol-phospholipase C (PI-PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5

The YvfTU Two-component System is involved in plcR expression in Bacillus cereus

Directory of Open Access Journals (Sweden)

Nguyen-the Christophe

2008-10-01

Full Text Available Abstract Background Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative role of YvfTU in the expression of the PlcR regulon was therefore investigated. Results Expression of the plcR gene was monitored using a transcriptional fusion with a lacZ reporter gene in a yvfTU mutant and in its B. cereus ATCC 14579 parental strain. Two hours after the onset of the stationary phase, a stage at which the PlcR regulon is highly expressed, the plcR expression in the yvfTU mutant was only 50% of that of its parental strain. In addition to the reduced plcR expression in the yvfTU mutant, a few members of the PlcR regulon showed a differential expression, as revealed by transcriptomic and proteomic analyses. The virulence of the yvfTU mutant in a Galleria mellonella insect model was slightly lower than that of the parental strain. Conclusion The YvfTU two-component system is not required for the expression of most of the virulence factors belonging to the PlcR regulon. However, YvfTU is involved in expression of plcR, a major regulator of virulence in B. cereus.

77 FR 32007 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2012-05-31

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... all Rolls-Royce plc (RR) RB211-Trent 800 series turbofan engines. This AD requires removal from...-17, 877- 17, 884-17, 884B-17, 892-17, 892B-17, and 895-17 turbofan engines. (d) Reason This AD was...

78 FR 6749 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-01-31

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... (AD) for all Rolls-Royce plc (RR) models RB211 Trent 768-60, 772-60, and 772B-60 turbofan engines... 772B-60 turbofan engines. (d) Reason This AD was prompted by low-pressure (LP) compressor blade partial...

Upgradation in SCADA and PLC of existing LN_2 control system for SST-1

International Nuclear Information System (INIS)

Panchal, Pradip; Mahesuria, Gaurang; Panchal, Rohit; Patel, Rakesh; Sonara, Dashrath; Pitroda, Dipen; Nimavat, Hiren; Tanna, Vipul; Pradhan, Subrata

2016-01-01

Highlights: • The control system of LN_2 Management System of SST-1 is designed on PLC and SCADA. • The implementation and results of up-gradation in PLC and SCADA are reported. • The up-gradation in PLC and SCADA has improved the reliability & availability of SST-1 LN_2 system. - Abstract: Helium Refrigerator/Liquefier system of Steady State Superconducting Tokamak (SST-1) incorporates Liquid Nitrogen (LN_2) pre-cooling system. LN_2 is used for 80 K thermal shields of SST-1, current feeder system and integrated flow distribution and control system. The LN_2 management system is distributed system and requires automatic control. Initially LN_2 control system had Citect based Supervisory Control and Data Acquisition (SCADA) and Koyo make Programmable Logic Controller (PLC). With the passage of time and due to unavailability of their hardware, it is being obsoleted. So, the requirements of new PLC and SCADA systems have been envisaged to make uninterruptable operation of SST-1 cryogenic system. Therefore, Wonderware SCADA and Schneider Electric make PLC is programmed to replace Citect SCADA and Koyo PLC. New control features have been added in upgraded control system for better management of LN_2 system. This upgradation of SCADA and PLC is completed, tested successfully and in operation. Operational performance highlights of the new upgraded system are presented in this paper.

Development and Application of POSAFE-Q PLC Platform

International Nuclear Information System (INIS)

Lee, MyeongKyun; Song, SeungWhan; Yun, DongHwa

2012-01-01

The Safety-grade Programmable Logic Controller (PLC) Platform named POSAFE-Q was developed so that it meets the requirements of the Safety Class 1E, Quality Class 1, and Seismic Category I. Development process of the POSAFE-Q software developed in accordance with software life cycles. The POSAFE-Q meets safety and suitability for design are based on digital I and C laws, guidelines and technical standards. The POSAFE-Q PLC obtained approval for Topical Report from the Nuclear Safety and Security Commission in Korea. (author)

Towards Designing PLC Networks for Ubiquitous Connectivity in Enterprises

OpenAIRE

Ali, Kamran; Pefkianakis, Ioannis; Liu, Alex X.; Kim, Kyu-Han

2016-01-01

Powerline communication (PLC) provides inexpensive, secure and high speed network connectivity, by leveraging the existing power distribution networks inside the buildings. While PLC technology has the potential to improve connectivity and is considered a key enabler for sensing, control, and automation applications in enterprises, it has been mainly deployed for improving connectivity in homes. Deploying PLCs in enterprises is more challenging since the power distribution network is more com...

Application of PLC in the crane control system of MJTR

International Nuclear Information System (INIS)

Li Ziqiang; Ji Xiangdong

2004-01-01

The paper describes the application of PLC (Programmable Controller) in the control system of the bridge crane. PLC is the essential part of the control system, which uses some equipment such as frequency transformer and photoelectric switch to implement remote, manual and automatic centering functions. This paper emphasizes the programming of the automatic hole centering

Wavelet-OFDM Signal Transmission Characteristics with High-Speed PLC Modem

Science.gov (United States)

Nakagawa, Kenichi; Tokuda, Masamitsu; Igata, Yuji

In this paper, we measured the interference immunity characteristics of high-speed PLC system using Wavelet-OFDM when the narrowband conducted interference wave signal was injected. As the results, it was clear that (1) measured PHY rate at the all frequency band hardly decreased in C/I (Carrier to Interference ratio) of above 20dB, but began to decrease rapidly in C/I of below 0dB when the interference signal was injected in the frequency band of high-speed PLC signal, (2) when C/I became from 0dB to -20dB, the measured PHY rate at the frequency existing the notch band were improved around 10Mbps than that at the frequency not existing the notch band, (3) when the narrowband interference wave was injected outside of frequency band of high-speed PLC signal, the measured PHY rate did not decrease than that in each notch band. Therefore, it was revealed that high-speed PLC system using Wavelet-OFDM had good interference immunity characteristics.

Machine interlock and protection system based on PLC for the SSRF linac

International Nuclear Information System (INIS)

Chou Wenjun; Zhou Dayong; Chen Jianfeng; Shen Liren; Liu Yajuan

2008-01-01

This paper describes a machine interlock and protection system used for accelerators based on EPICS (Experimental physics and industrial control system). The system is composed of a front-end computer and an FM-3R logic controller PLC. The alarm signal is passed by the hardware directly, and would be deal with PLC. The reporting, recording and analyst of the event are accomplished by EPICS control software. And PLC is linked to the EPICS by Internet. (authors)

77 FR 75831 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2012-12-26

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... certain serial numbers (S/Ns) of Rolls-Royce plc (RR) RB211-Trent 768-60, 772- 60, and 772B-60 turbofan... use any of the RB211-Trent 768-60, 772-60, and 772B-60 turbofan engines listed by S/N in this AD...

Application of Kingview and PLC in friction durability test system

Science.gov (United States)

Gao, Yinhan; Cui, Jing; Yang, Kaiyu; Ke, Hui; Song, Bing

2013-01-01

Using PLC and Kingview software, a friction durability test system is designed. The overall program, hardware configuration, software structure and monitoring interface are described in detail. PLC ensures the stability of data acquisition, and the KingView software makes the HMI easy to manipulate. The practical application shows that the proposed system is cheap, economical and highly reliable.

76 FR 65136 - Airworthiness Directives; Rolls-Royce plc (RR) Turbofan Engines

Science.gov (United States)

2011-10-20

... Airworthiness Directives; Rolls-Royce plc (RR) Turbofan Engines AGENCY: Federal Aviation Administration (FAA... information identified in this AD, contact Rolls-Royce plc, Corporate Communications, P.O. Box 31, Derby... 8, 2011, to perform the inspection. Costs of Compliance Based on the service information, we...

78 FR 54149 - Airworthiness Directives; Rolls-Royce plc (RR) Turbofan Engines

Science.gov (United States)

2013-09-03

... Airworthiness Directives; Rolls-Royce plc (RR) Turbofan Engines AGENCY: Federal Aviation Administration (FAA... service information identified in this AD, contact Rolls-Royce plc, Corporate Communications, P.O. Box 31... per hour. Replacement parts are estimated to cost about $2,271 per engine. Based on these figures, we...

Normal and pathological NMR imaging aspects of the posterolateral corner (PLC) of the knee

International Nuclear Information System (INIS)

Tardieu, M.; Lazennec, J.Y.; Christel, P.; Brasseur, J.L.; Roger, B.; Grenier, P.

1995-01-01

The purpose of the study is to compare normal PLC (limits lateral condyle anterior sub luxation) anatomy and its magnetic resonance imaging (MRI) appearance, with the various lesions observed in MRI, from the simple popliteus tendinous contusion to the complete PLC rupture. For this specific work on PLC lesions, we selected 61 examinations among the traumatic knees explored during the last 3 years. Surgical correlation is obtained for the 61 patients. MRI examinations are performed on a 0.5 T. unit. Normal PLC anatomy is compared to the dissection of 4 anatomic subjects. Normal MRI slices are evaluated with this reference analysis. The principle anatomical structures of the PLC include the lateral collateral ligament, the popliteus tendon, the arcuate ligament, the fabello fibular ligament, the posterolateral condylar capsule, and the posterior horn of the lateral meniscus. Surgical findings confirm PLC lesion for 58 patients with 3 false positive. Diagnosis of these lesions is important because chronical posterolateral laxity is secondary to the destabilization of lateral condyle. Unrecognized and untreated posterolateral instability may result in failure of ACL (limits lateral condyle posterior sub-luxation) reconstruction. When clinical tests are doubtful or complex, or the examination very painful, MRI evaluates completely the traumatic knee and particularly the PLC. (authors). 3 refs., 26 figs

Fc gamma receptor activation induces the tyrosine phosphorylation of both phospholipase C (PLC)-gamma 1 and PLC-gamma 2 in natural killer cells

OpenAIRE

1992-01-01

Crosslinking of the low affinity immunoglobulin G (IgG) Fc receptor (Fc gamma R type III) on natural killer (NK) cells initiates antibody- dependent cellular cytotoxicity. During this process, Fc gamma R stimulation results in the rapid activation of phospholipase C (PLC), which hydrolyzes membrane phosphoinositides, generating inositol-1,4,5- trisphosphate and sn-1,2-diacylglycerol as second messengers. We have recently reported that PLC activation after Fc gamma R stimulation can be inhibit...

Design and implementation of tutorials for PLC B&R Automation

OpenAIRE

Sýkora, Daniel

2014-01-01

Tato bakalářská práce se zabývá návrhem a realizací úloh pro PLC B&R a podrobným návodem práce v Automation Studio. Obsahuje také informace o firmě B&R Automation Ges. m. b. H. a jejích produktech. Návrh úloh probíhal ve vývojovém prostředí B&R Automation Studio. Praktická úloha na regulaci teploty byla řešena a odzkoušena na B&R PLC X20. This bachelor’s thesis deals with the design and the implementation tutorials for PLC B&R as well as the detailed instructions of work in the Automation ...

The Design and Realization of Virtual Machine of Embedded Soft PLC Running System

Directory of Open Access Journals (Sweden)

Qingzhao Zeng

2014-11-01

Full Text Available Currently soft PLC has been the focus of study object for many countries. Soft PLC system consists of the developing system and running system. A Virtual Machine is an important part in running system even in the whole soft PLC system. It explains and performs intermediate code generated by the developing system and updates I/O status of PLC in order to complete its control function. This paper introduced the implementation scheme and execution process of the embedded soft PLC running system Virtual Machine, and mainly introduced its software implementation method, including the realization of the input sampling program, the realization of the instruction execution program and the realization of output refresh program. Besides, an operation code matching method was put forward in the instruction execution program design. Finally, the test takes PowerPC/P1010 (Freescale as the hardware platform and Vxworks as the operating system, the system test result shows that accuracy, the real-time performance and reliability of Virtual Machine.

Application of PLC to mine hoist

International Nuclear Information System (INIS)

Zheng Liquan

2003-01-01

This paper describes the reform of the hoister JMK 1.85 x 4 (B) with programmable logic controller (PLC), introduces hardware architecture, software architecture and interference protection measures, and presents the existential problems and suggestions for improvement

Voltage Control System of A DC Generator Using PLC

OpenAIRE

Subrata CHATTOPADHYAY; Sagarika PAL

2008-01-01

The voltage control system of a DC generator may suffer from high frequency oscillations without offset or low frequency oscillation with offset. A PID controller can eliminate both these errors. In the present paper, the voltage control system of a DC generator using a PLC based PID controller has been designed. Operation of PLC as a continuous controller has been described and the load characteristic of DC generator with and without controller have been determined experimentally and reporte...

Intelligent Traffic Light Based on PLC Control

Science.gov (United States)

Mei, Lin; Zhang, Lijian; Wang, Lingling

2017-11-01

The traditional traffic light system with a fixed control mode and single control function is contradicted with the current traffic section. The traditional one has been unable to meet the functional requirements of the existing flexible traffic control system. This paper research and develop an intelligent traffic light called PLC control system. It uses PLC as control core, using a sensor module for receiving real-time information of vehicles, traffic control mode for information to select the traffic lights. Of which control mode is flexible and changeable, and it also set the countdown reminder to improve the effectiveness of traffic lights, which can realize the goal of intelligent traffic diversion, intelligent traffic diversion.

A formal safety analysis for PLC software-based safety critical system using Z

International Nuclear Information System (INIS)

Koh, Jung Soo

1997-02-01

This paper describes a formal safety analysis technique which is demonstrated by performing empirical formal safety analysis with the case study of beamline hutch door Interlock system that is developed by using PLC (Programmable Logic Controller) systems at the Pohang Accelerator Laboratory. In order to perform formal safety analysis, we have built the Z formal specifications representation from user requirement written in ambiguous natural language and target PLC ladder logic, respectively. We have also studied the effective method to express typical PLC timer component by using specific Z formal notation which is supported by temporal history. We present a formal proof technique specifying and verifying that the hazardous states are not introduced into ladder logic in the PLC-based safety critical system. And also, we have found that some errors or mismatches in user requirement and final implemented PLC ladder logic while analyzing the process of the consistency and completeness of Z translated formal specifications. In the case of relatively small systems like Beamline hutch door interlock system, a formal safety analysis including explicit proof is highly recommended so that the safety of PLC-based critical system may be enhanced and guaranteed. It also provides a helpful benefits enough to comprehend user requirement expressed by ambiguous natural language

Implementation of PID autotuning procedure in PLC controller

Directory of Open Access Journals (Sweden)

Daniun Marcin

2017-01-01

Full Text Available In this paper, we present the automatic PID tuning procedure based on the Method of Moments and AMIGO tuning rules. The advantage of the Method of Moments is that the time constant and transport delay are estimated at the areas rather than on the individual points. This results in high resistance to the measurement noises. The sensitivity to measurement noises is a serious problem in other autotuning methods. The second advantage of this method is that it approximates plant during identification process to first order model with time delay. We combined the Method of Moments with the AMIGO tuning rules and implemented this combination as a stand-alone autotuning procedure in Siemens S7-1200 PLC controller. Next, we compared this method with two built-in PID autotuning procedures which were available in Siemens S7-1200 PLC controller. The procedure was tested for three types of plant models: with lag-dominated, balanced, and delay-dominated dynamics. We simulated the plants on a PC in Matlab R2013a. The connection between the PC and PLC was maintained through a National Instruments data acquisition board, NI PCI-6229. We conducted tests for step change in the set point, trajectory tracking, and load disturbances. To assess control quality, we used IAE index. We limited our research to PI algorithm. The results prove that proposed method was better than two built-in tuning methods provided by Siemens, oscillating between a few and even a dozen percent in most cases. The proposed method is universal and can be implemented in any PLC controller.

Upgradation in SCADA and PLC of existing LN{sub 2} control system for SST-1

Energy Technology Data Exchange (ETDEWEB)

Panchal, Pradip, E-mail: pradip@ipr.res.in; Mahesuria, Gaurang; Panchal, Rohit; Patel, Rakesh; Sonara, Dashrath; Pitroda, Dipen; Nimavat, Hiren; Tanna, Vipul; Pradhan, Subrata

2016-11-15

Highlights: • The control system of LN{sub 2} Management System of SST-1 is designed on PLC and SCADA. • The implementation and results of up-gradation in PLC and SCADA are reported. • The up-gradation in PLC and SCADA has improved the reliability & availability of SST-1 LN{sub 2} system. - Abstract: Helium Refrigerator/Liquefier system of Steady State Superconducting Tokamak (SST-1) incorporates Liquid Nitrogen (LN{sub 2}) pre-cooling system. LN{sub 2} is used for 80 K thermal shields of SST-1, current feeder system and integrated flow distribution and control system. The LN{sub 2} management system is distributed system and requires automatic control. Initially LN{sub 2} control system had Citect based Supervisory Control and Data Acquisition (SCADA) and Koyo make Programmable Logic Controller (PLC). With the passage of time and due to unavailability of their hardware, it is being obsoleted. So, the requirements of new PLC and SCADA systems have been envisaged to make uninterruptable operation of SST-1 cryogenic system. Therefore, Wonderware SCADA and Schneider Electric make PLC is programmed to replace Citect SCADA and Koyo PLC. New control features have been added in upgraded control system for better management of LN{sub 2} system. This upgradation of SCADA and PLC is completed, tested successfully and in operation. Operational performance highlights of the new upgraded system are presented in this paper.

Model-based automated testing of critical PLC programs.

CERN Document Server

Fernández Adiego, B; Tournier, J-C; González Suárez, V M; Bliudze, S

2014-01-01

Testing of critical PLC (Programmable Logic Controller) programs remains a challenging task for control system engineers as it can rarely be automated. This paper proposes a model based approach which uses the BIP (Behavior, Interactions and Priorities) framework to perform automated testing of PLC programs developed with the UNICOS (UNified Industrial COntrol System) framework. This paper defines the translation procedure and rules from UNICOS to BIP which can be fully automated in order to hide the complexity of the underlying model from the control engineers. The approach is illustrated and validated through the study of a water treatment process.

A formal safety analysis for PLC software-based safety critical system using Z

International Nuclear Information System (INIS)

Koh, Jung Soo; Seong, Poong Hyun

1997-01-01

This paper describes a formal safety analysis technique which is demonstrated by performing empirical formal safety analysis with the case study of beamline hutch door Interlock system that is developed by using PLC (Programmable Logic Controller) systems at the Pohang Accelerator Laboratory. In order to perform formed safety analysis, we have built the Z formal specifications representation from user requirement written in ambiguous natural language and target PLC ladder logic, respectively. We have also studied the effective method to express typical PLC timer component by using specific Z formal notation which is supported by temporal history. We present a formal proof technique specifying and verifying that the hazardous states are not introduced into ladder logic in the PLC-based safety critical system

MultiController: the PLC-SCADA object for advanced regulation

CERN Document Server

Ortola, J

2007-01-01

Nowadays, industrial solutions with PLCs (Programmable Logic Controllers) have basic control loop features. The SCADA (Supervisory Control And Data Acquisition) system is a key point of the process control system due to an efficient HMI (Human Machine Interfaces) that provides an open method of tuning and leading possibilities. As a consequence, advanced control algorithms have to be developed and implemented for those PLC-SCADA solutions in order to provide perspectives in solving complex and critical regulation problems. The MultiController is an object integrated for a large scale project at CERN (the European Organization for Nuclear Research) named LHC-GCS (Large Hadron Collider - Gas Control System). It is developed for a Framework called CERN-UNICOS based on PLC-SCADA facilities. The MultiController object offers various advanced control loop strategies. It gives to the user advanced control algorithms like PID, Smith Predictor, PFC, GPC and RST. It is implemented as a monolithic entity (in PLC and SCA...

Separation of integrin-dependent adhesion from morphological changes based on differential PLC specificities.

Science.gov (United States)

Wooten, D K; Teague, T K; McIntyre, B W

1999-01-01

In normal lymphocytes an inside-out signal up-regulating integrin adhesion is followed by a ligand-mediated outside-in cell spreading signal. Protein kinase C (PKC) inhibition blocks lymphocyte adherence to and spreading on fibronectin. In contrast, putative PLC inhibitors yield distinct differences with respect to adhesion and morphology. The phosphatidylinositol-specific phospholipase C (PLC) inhibitor neomycin blocked spreading of CD3/CD28-activated T cells on fibronectin by disrupting adhesion. Furthermore, when an additional inside-out signal for fibronectin adhesion is unnecessary such as with HPB-ALL T leukemic or phorbol-myristate-acetate-treated normal T cells, neomycin treatment does not alter adhesion or morphology. However, the phosphatidylcholine-specific PLC inhibitor D609 abrogates cell spreading without affecting adhesion to fibronectin in these cells as well as the CD3/CD28-activated T cells. These results strongly suggest that inside-out signaling for the integrin alpha4beta1 in lymphocytes proceeds through phosphatidylinositol-specific PLC and PKC, whereas the outside-in signal utilizes phosphatidylcholine-specific PLC and PKC.

Voltage Control System of A DC Generator Using PLC

Directory of Open Access Journals (Sweden)

Subrata CHATTOPADHYAY

2008-06-01

Full Text Available The voltage control system of a DC generator may suffer from high frequency oscillations without offset or low frequency oscillation with offset. A PID controller can eliminate both these errors. In the present paper, the voltage control system of a DC generator using a PLC based PID controller has been designed. Operation of PLC as a continuous controller has been described and the load characteristic of DC generator with and without controller have been determined experimentally and reported in this paper.

Transforming PLC Programs into Formal Models for Verification Purposes

CERN Document Server

Darvas, D; Blanco, E

2013-01-01

Most of CERN’s industrial installations rely on PLC-based (Programmable Logic Controller) control systems developed using the UNICOS framework. This framework contains common, reusable program modules and their correctness is a high priority. Testing is already applied to find errors, but this method has limitations. In this work an approach is proposed to transform automatically PLC programs into formal models, with the goal of applying formal verification to ensure their correctness. We target model checking which is a precise, mathematical-based method to check formalized requirements automatically against the system.

Performance analysis of the PLC (Power Line Communication) in medium voltage electrical networks; Analise de desempenho de sistemas PLC em redes eletricas de media tensao

Energy Technology Data Exchange (ETDEWEB)

Mota, A.A.; Paleta, R. [Pontificia Universidade Catolica de Campinas (PUC-Campinas), SP (Brazil)], E-mail: amota@puc-campinas.edu.br; Mota, L.T.M.; Ricardo, R.A. [Indelmatec Engenharia, Campinas, SP (Brazil)], E-mail: mota@indelmatec.com.br

2009-07-01

Nowadays, the information access in communication networks is widely explored due to the increase of Internet users. In this context, the PLC (Power Line Communication) technology is an alternative for data transmission. This technology is based on the usage of transmission/distribution power lines for data transmission. However there are some problems related to the usage of this technology: adequate data transmission rates and generation of acceptable levels of electromagnetic interference (EMI). This work had the objective of studying the performance of PLC systems in medium voltage electrical networks, through the assess of data transmission rates and the generated EMI. Tests were carried out in a test field that corresponded to a medium voltage electrical network and the obtained results show that, under some circumstances, the PLC system does not reach the existent technical recommendations. (author)

File list: Pol.Plc.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

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File list: Pol.Plc.10.RNA_polymerase_II.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

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File list: Pol.Plc.05.RNA_polymerase_II.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

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File list: Pol.Plc.20.RNA_polymerase_II.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

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File list: Pol.Plc.05.RNA_polymerase_III.AllCell [Chip-atlas[Archive

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File list: Pol.Plc.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available Pol.Plc.50.RNA_polymerase_III.AllCell hg19 RNA polymerase RNA polymerase III Placen...ta http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Pol.Plc.50.RNA_polymerase_III.AllCell.bed ...

75 FR 46864 - Airworthiness Directives; Short Brothers PLC Model SD3 Airplanes

Science.gov (United States)

2010-08-04

...-0225; Directorate Identifier 2009-NM-203-AD] RIN 2120-AA64 Airworthiness Directives; Short Brothers PLC... this proposed AD, contact Short Brothers PLC, Airworthiness, P.O. Box 241, Airport Road, Belfast, BT3... amend this proposed AD based on those comments. We will post all comments we receive, without change, to...

Fifth Baltic Sea pollution load compilation (PLC-5)

Energy Technology Data Exchange (ETDEWEB)

Knuuttila, S.; Svendsen, L. M.; Staaf, H.; Kotilainen, P.; Boutrup, S.; Pyhala, M.; Durkin, M.

2011-07-01

This report includes the main results from the Fifth Pollution Load Compilation abbreviated PLC-5. It includes quantified annual waterborne total loads (from rivers, unmonitored and coastal areas as well as direct point and diffuse sources discharging directly to the Baltic Sea) from 1994 to 2008 to provide a basis for evaluating any decreasing (or increasing) trends in the total waterborne inputs to the Baltic Sea. Chapter 1 contains the objectives of PLC and the framework on classification of inputs and sources. Chapter 2 includes a short description of the Baltic Sea catchment area, while the methods for quantification and analysis together with quality assurance topics are briefly introduced in Chapter 3. More detailed information on methodologies is presented in the PLC-5 guidelines (HELCOM 2006). Chapter 4 reports the total inputs to the Baltic Sea of nutrients and selected heavy metals. Furthermore, the results of the quatification of discharges and losses of nitrogen and phosphorus from point and diffuse sources into inland surface waters within the Baltic Sea catchment area (source-oriented approach or gross loads) as well as the total load to the maritime area (load-oriented approarch or net loads) in 2006 are shown. Typically, results are presented by country and by main Baltic Sea sub-region. In Chapter 5, flow normalization is introduced and the results of trend analyses on 1994-2008 time series data on total waterborne loads of nitrogen and phosphorus are given together with a first evaluation of progress in obtaining the provisional reduction targets by country and by main Baltic Sea sub-region. Chapter 6 includes discussion of some of the main conclusions and advice for future PLCs. The annexes contain the flow-normalized annual load data and figures and tables with results from the PLC-5.

PLC and DTAM Software Programs for Pumping Instrumentation and Control Skid N

International Nuclear Information System (INIS)

KOCH, M.R.

2000-01-01

This document describe the software programs for the Programmable Logic Controller and the Datable Access Module for Pumping Instrumentation and Control skid ''N''. The Appendices contains copies of the printouts of these software programs. This document describes the software programs for the Allen-Bradley SLC 500 programmable logic controller (PLC) and the Allen-Bradley DTAM PLUS operator interface module used on Pumping Instrumentation and Control (PIC) skid ''N''. The software programs for the SLC 500 and DTAM Plus are based on the core programs provided by Allen-Bradley. The PLC and DTAM software programs on skid ''D'' for SX-104 are the baseline programs. These baselines have been tailored for U-farm skids. The skid ''N'' program for U-109 is similar to the skid ''M'' program for U-102. An Acceptance Test Procedure (ATP) and an Operational Test Procedure (OTP) verify that the software programs meet the specific requirements for U-109 pumping. This document represents the final PLC and DTAM programs for PIC skid ''N'' at U-109. These programs were printed out after the performance of the OTP. The OTP acts as the final qualification test for the software programs. Functional requirements and details of the PLC ladder logic are described in this document. The final programs entered into the PLC and DTAM Plus are included as Appendices to this document

File list: InP.Plc.50.Input_control.AllCell [Chip-atlas[Archive

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Full Text Available InP.Plc.50.Input_control.AllCell hg19 Input control Input control Placenta SRX19004...9,SRX080366 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Plc.50.Input_control.AllCell.bed ...

File list: InP.Plc.05.Input_control.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available InP.Plc.05.Input_control.AllCell hg19 Input control Input control Placenta SRX19004...9,SRX080366 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Plc.05.Input_control.AllCell.bed ...

Integration of the HVCM PLC into the PEFP Control System

International Nuclear Information System (INIS)

Song, Young Gi; Jang, Ji Ho; Kwon, Hyeok Jung; Cho, Yong Sub

2011-01-01

The High Voltage Converter Modulators (HVCM) for the 100-MeV accelerator was installed to drive the 20-MeV linac. There are two klystrons in the 20-MeV linac and one modulator was used to drive two klystrons simultaneously. The HVCM for the 20-MeV linac area in Korea Atomic Energy Research Institute (KAERI) is shown in Fig. 1. We were faced with the necessity of integrating Programmable Logic Controllers (PLCs) for the HVCM into the Proton Engineering Frontier Project (PEFP) control system. At the PEFP, Experimental Physics and Industrial Control System (EPICS) has become the most widely used solution for building control systems for 100MeV proton accelerator. The EPICS as a standard development tool is a distributed architecture that provides several solutions such as independent programming tools for operating system, operator interface tools, and archiving tools. Although EPICS is used directly in our control system, HVCMs were delivered with the Allen-Bradley ControlLogix as a PLC. The industrial PLC has been verified for safety systems. We need to connect an interface from our EPICS control system to AB-PLC using Ethernet/IP (ControlNet over Ethernet) protocol over Ethernet. In this paper, we will present the communication protocol and EPICS IOC installation for the EPICS based PLC control system

PLC-controlled cryostats for the BlackGEM and MeerLICHT detectors

Science.gov (United States)

Raskin, Gert; Morren, Johan; Pessemier, Wim; Bloemen, Steven; Klein-Wolt, Marc; Roelfsema, Ronald; Groot, Paul; Aerts, Conny

2016-08-01

BlackGEM is an array of telescopes, currently under development at the Radboud University Nijmegen and at NOVA (Netherlands Research School for Astronomy). It targets the detection of the optical counterparts of gravitational waves. The first three BlackGEM telescopes are planned to be installed in 2018 at the La Silla observatory (Chile). A single prototype telescope, named MeerLICHT, will already be commissioned early 2017 in Sutherland (South Africa) to provide an optical complement for the MeerKAT radio array. The BlackGEM array consists of, initially, a set of three robotic 65-cm wide-field telescopes. Each telescope is equipped with a single STA1600 CCD detector with 10.5k x 10.5k 9-micron pixels that covers a 2.7 square degrees field of view. The cryostats for housing these detectors are developed and built at the KU Leuven University (Belgium). The operational model of BlackGEM requires long periods of reliable hands-off operation. Therefore, we designed the cryostats for long vacuum hold time and we make use of a closed-cycle cooling system, based on Polycold PCC Joule-Thomson coolers. A single programmable logic controller (PLC) controls the cryogenic systems of several BlackGEM telescopes simultaneously, resulting in a highly reliable, cost-efficient and maintenance-friendly system. PLC-based cryostat control offers some distinct advantages, especially for a robotic facility. Apart of temperature monitoring and control, the PLC also monitors the vacuum quality, the power supply and the status of the PCC coolers (compressor power consumption and temperature, pressure in the gas lines, etc.). Furthermore, it provides an alarming system and safe and reproducible procedures for automatic cool down and warm up. The communication between PLC and higher-level software takes place via the OPC-UA protocol, offering a simple to implement, yet very powerful interface. Finally, a touch-panel display on the PLC provides the operator with a user-friendly and robust

Realization of PLC to the Variable Frequency Speed Regulation System of Mine Local Ventilator based on RS-485 Communication

Science.gov (United States)

Ma, Kai; Li, Jian; Yun, Yichong

2018-03-01

The article first introduces the merits of serial communication in the PLC to the variable frequency speed regulation system of mine local ventilator, and then sets up a hardware application development platform of PLC and inverter based on RS-485 communication technology, next presents communication initialization of the PLC and Inverter. Finally according to the control requirements, PLC send run operation & monitoring instruction to Inverter, realizes the serial communication control between the PLC and Inverter.

Automated Translation of Safety Critical Application Software Specifications into PLC Ladder Logic

Science.gov (United States)

Leucht, Kurt W.; Semmel, Glenn S.

2008-01-01

The numerous benefits of automatic application code generation are widely accepted within the software engineering community. A few of these benefits include raising the abstraction level of application programming, shorter product development time, lower maintenance costs, and increased code quality and consistency. Surprisingly, code generation concepts have not yet found wide acceptance and use in the field of programmable logic controller (PLC) software development. Software engineers at the NASA Kennedy Space Center (KSC) recognized the need for PLC code generation while developing their new ground checkout and launch processing system. They developed a process and a prototype software tool that automatically translates a high-level representation or specification of safety critical application software into ladder logic that executes on a PLC. This process and tool are expected to increase the reliability of the PLC code over that which is written manually, and may even lower life-cycle costs and shorten the development schedule of the new control system at KSC. This paper examines the problem domain and discusses the process and software tool that were prototyped by the KSC software engineers.

Prototype of Automated PLC Model Checking Using Continuous Integration Tools

CERN Document Server

Lettrich, Michael

2015-01-01

To deal with the complexity of operating and supervising large scale industrial installations at CERN, often Programmable Logic Controllers (PLCs) are used. A failure in these control systems can cause a disaster in terms of economic loses, environmental damages or human losses. Therefore the requirements to software quality are very high. To provide PLC developers with a way to verify proper functionality against requirements, a Java tool named PLCverif has been developed which encapsulates and thus simplifies the use of third party model checkers. One of our goals in this project is to integrate PLCverif in development process of PLC programs. When the developer changes the program, all the requirements should be verified again, as a change on the code can produce collateral effects and violate one or more requirements. For that reason, PLCverif has been extended to work with Jenkins CI in order to trigger automatically the verication cases when the developer changes the PLC program. This prototype has been...

PROTEST AND CONSUMER: A CONTENT ANALYSIS OF EBSCO ELECTRONIC DATABASE ACROSS 50 YEARS

Directory of Open Access Journals (Sweden)

Kresno A. Hendarto

2014-02-01

Full Text Available AbstractThe objective of this research is to review the articles of protest and consumer in EBSCO electronic journal. The objective will be achieved by answering the following questions: (i what is the method of reviewed articles with the topic of protest; and (ii what agenda of future research? The results showed that most reviewed articles  used qualitative approach. Most research are empirical in nature. For articles of empirical research, most of the data were obtained through observation and were analyzed descriptively. Literature on protest and consumer can be classified into 3 main categories: (1 discussing about the frequency, cause, and objective of protest; (2 discussing about the consequence of protest; and (3 discussing about the  motivation of individuals in taking part in the protest. This review also showed that there are major gaps from the previous research including the fact that attitude is not the focal point in explaining why consumers take part in protest and no article discussed consumers’ attitude to the object taking part in the protest.  AbstrakTujuan penelitian ini adalah melakukan ulas balik artikel-artikel bertopik protes dan konsumen pada database elektronik EBSCO. Tujuan tersebut akan dicapai dengan menjawab pertanyaan penelitian sebagai berikut: (i apa metode dan sifat dasar dari artikel yang bertopik protes; dan (ii agenda penelitian apa yang masih terbuka ke depan? Hasil analisis menunjukkan bahwa kebanyakan artikel yang direview menggunakan pendekatan kualitatif. Kebanyakan artikel merupakan penelitian empiris. Untuk artikel penelitian empiris, mayoritas data diperoleh dengan cara observasi, dan dianalisis secara deskriptif. Literatur tentang protes dan konsumen dapat digolongkan dalam 3 bagian besar, yaitu (1 yang membahas tentang frekuensi, sebab, dan tujuan protes; (2 yang membahas tentang konsekuensi dari protes; dan (3 yang membahas tentang motivasi individu yang mendasari untuk berpartisipasi dalam

Evaluation of PLC Channel Capacity and ABER Performances for OFDM-Based Two-Hop Relaying Transmission

Directory of Open Access Journals (Sweden)

Sana Ezzine

2017-01-01

Full Text Available Powerline network is recognized as a favorable infrastructure for Smart Grid to transmit information in the network thanks to its broad coverage and low cost deployment. The existing works are trying to improve and adapt transmission techniques to reduce Powerline Communication (PLC channel attenuation and exploit the limited bandwidth to support high data rate over long distances. Two-hop relaying BroadBand PLC (BB-PLC system, in which Orthogonal Frequency Division Multiplexing (OFDM is used, is considered in this paper. We derive and compare the PLC channel capacity and the end-to-end Average BER (ABER for OFDM-based direct link (DL BB-PLC system and for OFDM-based two-hop relaying BB-PLC system for Amplify and Forward (AF and Decode and Forward (DF protocols. We analyze the improvements when we consider the direct link in a cooperative communication when the relay node only transmits the correctly decoded signal. Maximum ratio combining is employed at the destination node to detect the transmitted signal. In addition, in this paper, we highlight the impact of the relay location on the channel capacity and ABER for AF and DF transmission protocols. Moreover, an efficient use of the direct link was also investigated in this paper.

Bit rate maximization for multicast LP-OFDM systems in PLC context

OpenAIRE

Maiga , Ali; Baudais , Jean-Yves; Hélard , Jean-François

2009-01-01

ISBN: 978-88-900984-8-2.; International audience; In this paper, we propose a new resource allocation algorithm based on linear precoding technique for multicast OFDM systems. Linear precoding technique applied to OFDM systems has already proved its ability to significantly increase the system throughput in a powerline communication (PLC) context. Simulations through PLC channels show that this algorithm outperforms the classical multicast method (up to 7.3% bit rate gain) and gives better pe...

76 FR 80430 - Rio Tinto plc and Rio Tinto Limited; Notice of Application

Science.gov (United States)

2011-12-23

... plc and Rio Tinto Limited; Notice of Application December 19, 2011. AGENCY: Securities and Exchange Commission (``Commission''). ACTION: Notice of application under section 3(b)(2) and 45(a) of the Investment Company Act of 1940 (the ``Act''). SUMMARY: Summary of Application: Rio Tinto plc (``RTP'') and Rio Tinto...

Evaluation of PLC Channel Capacity and ABER Performances for OFDM-Based Two-Hop Relaying Transmission

OpenAIRE

Ezzine, Sana; Abdelkefi, Fatma; Cances, Jean Pierre; Meghdadi, Vahid; Bouallégue, Ammar

2017-01-01

Powerline network is recognized as a favorable infrastructure for Smart Grid to transmit information in the network thanks to its broad coverage and low cost deployment. The existing works are trying to improve and adapt transmission techniques to reduce Powerline Communication (PLC) channel attenuation and exploit the limited bandwidth to support high data rate over long distances. Two-hop relaying BroadBand PLC (BB-PLC) system, in which Orthogonal Frequency Division Multiplexing (OFDM) is u...

Connecting programmable logic controllers (PLC) to control and data acquisition a comparison of the JET and Wendelstein 7-X approach

International Nuclear Information System (INIS)

Hennig, Christine; Kneupner, Klaus; Kinna, David

2012-01-01

Highlights: ► We describe 2 ways connecting PLCs to fusion control and data acquisition software. ► At W7-X standardization of the PLC type eases the maintenance of the software. ► At JET PLCs are interfaced with a daemon that hides the PLC specific part. ► There is potential to unify the approaches towards a common fusion PLC interface. - Abstract: The use of programmable logic controllers (PLC) for automation of electromechanical processes is an industrial control system technology. It is more and more in use within the fusion community. Traditionally PLC based systems are operated and maintained using proprietary SCADA systems (supervisory control and data acquisition). They are hardly ever integrated with the fusion control and data acquisition systems. An overview of the state of the art in fusion is given in the article. At JET an inhouse “black box protocol” approach has been developed to communicate with any external system via a dedicated http based protocol. However, a PLC usually cannot be modified to implement this special protocol. Hence, a software layer has been developed that interfaces a PLC by implementing the PLC specific communication part on one side and the black box protocol part on the other side. The software is completely data driven i.e. editing the data structure changes the logic accordingly. It can be tested using the web capability of the black box protocol. Multiple PLC types from different vendors are supported, thus multiple protocols to interface the PLC are in use. Depending on the PLC type and available tools it can be necessary to program the PLC accordingly. Wendelstein 7-X uses another approach. For every single PLC a dedicated communication from and to CoDaC is implemented. This communication is projected (programmed) in the PLC and configurable (data driven) on the CoDaC side. The protocol is UDP based and observed via timeout mechanisms. The use of PLCs for Wendelstein 7-X is standardized. Therefore a single

Connecting programmable logic controllers (PLC) to control and data acquisition a comparison of the JET and Wendelstein 7-X approach

Energy Technology Data Exchange (ETDEWEB)

Hennig, Christine, E-mail: Christine.Hennig@ipp.mpg.de [Max-Planck-Institut fuer Plasmaphysik, Wendelsteinstrasse 1, 17491 Greifswald (Germany); Kneupner, Klaus; Kinna, David [JET-EFDA, Culham Science Centre, OX14 3DB Abingdon (United Kingdom)

2012-12-15

Highlights: Black-Right-Pointing-Pointer We describe 2 ways connecting PLCs to fusion control and data acquisition software. Black-Right-Pointing-Pointer At W7-X standardization of the PLC type eases the maintenance of the software. Black-Right-Pointing-Pointer At JET PLCs are interfaced with a daemon that hides the PLC specific part. Black-Right-Pointing-Pointer There is potential to unify the approaches towards a common fusion PLC interface. - Abstract: The use of programmable logic controllers (PLC) for automation of electromechanical processes is an industrial control system technology. It is more and more in use within the fusion community. Traditionally PLC based systems are operated and maintained using proprietary SCADA systems (supervisory control and data acquisition). They are hardly ever integrated with the fusion control and data acquisition systems. An overview of the state of the art in fusion is given in the article. At JET an inhouse 'black box protocol' approach has been developed to communicate with any external system via a dedicated http based protocol. However, a PLC usually cannot be modified to implement this special protocol. Hence, a software layer has been developed that interfaces a PLC by implementing the PLC specific communication part on one side and the black box protocol part on the other side. The software is completely data driven i.e. editing the data structure changes the logic accordingly. It can be tested using the web capability of the black box protocol. Multiple PLC types from different vendors are supported, thus multiple protocols to interface the PLC are in use. Depending on the PLC type and available tools it can be necessary to program the PLC accordingly. Wendelstein 7-X uses another approach. For every single PLC a dedicated communication from and to CoDaC is implemented. This communication is projected (programmed) in the PLC and configurable (data driven) on the CoDaC side. The protocol is UDP based and

Application of PLC timing control in the neutral beam injector of HT-7

International Nuclear Information System (INIS)

Song Shihua; Liu Zhimin; Liu Sheng; Hu Chundong

2006-01-01

HT-7 tokamak high power Neutral Beam Injector heating system runs in the mode of pulse timing-control of PLC. The thesis discusses the theory about the operation for the experiment of discharge, which is controlled by PLC logical connection and introduces excellent user-friendly operating interface and the development of the ladder application program and upper monitor program in the VB6.0 environment. Monitor the conditions of power and facility real time by the upper monitor interface. The application of PLC control system ensures the experiment facility running safely and convenient for modifying and setting the parameter simply during the course of experiment. (authors)

Arbitration Award of ICSID on the Investment Disputes of Churchill Mining PLC v. Republic of Indonesia

Directory of Open Access Journals (Sweden)

Yordan Gunawan

2017-03-01

Full Text Available The research is aimed at analyzing the ICSID (International Centre Settlement Investment Dispute decision in solving a dispute between Churchill Mining PLC and the Government of the Republic of Indonesia. The case brought to the public attention, because mining license owned by PT. Ridlatama which acquired from Churchill Mining PLC had been revocated. Churchill Mining PLC holds 75% share of PT. Ridlatama and it suffered losses caused by the revocation of its mining license. Churchill Mining PLC filed the case to the local court but it failed. Churchill Mining PLC then sought ruling from International arbitration or ICSID. On December 6, 2016, ICSID issued a decision that clearly threw out Churchill Mining PLC claim. ICSID, the World Bank court, ordered the firm to pay a total of US$.9.446.528 in cost to the Government of the Republic of Indonesia. It is based on the evidences that the UK-Australia company did the fraud and had document forgery of coal mining permit in East Kutai, Indonesia. So the firm has violated the Bilateral Investment Treaties between Indonesia-UK and Indonesia-Australia.

The PLC: a logical development

OpenAIRE

Walker, Mark; Bissell, Christopher; Monk, John

2010-01-01

Programmable Logic Controllers (PLCs) have been used to control industrial processes and equipment for over 40 years, having their first commercially recognised application in 1969. Since then there have been enormous changes in the design and application of PLCs, yet developments were evolutionary rather than radical. The flexibility of the PLC does not confine it to industrial use and it has been used for disparate non-industrial control applications . This article reviews the history, deve...

File list: InP.Plc.20.Input_control.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

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File list: InP.Plc.05.Input_control.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

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File list: InP.Plc.10.Input_control.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

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PLAT: An Automated Fault and Behavioural Anomaly Detection Tool for PLC Controlled Manufacturing Systems.

Science.gov (United States)

Ghosh, Arup; Qin, Shiming; Lee, Jooyeoun; Wang, Gi-Nam

2016-01-01

Operational faults and behavioural anomalies associated with PLC control processes take place often in a manufacturing system. Real time identification of these operational faults and behavioural anomalies is necessary in the manufacturing industry. In this paper, we present an automated tool, called PLC Log-Data Analysis Tool (PLAT) that can detect them by using log-data records of the PLC signals. PLAT automatically creates a nominal model of the PLC control process and employs a novel hash table based indexing and searching scheme to satisfy those purposes. Our experiments show that PLAT is significantly fast, provides real time identification of operational faults and behavioural anomalies, and can execute within a small memory footprint. In addition, PLAT can easily handle a large manufacturing system with a reasonable computing configuration and can be installed in parallel to the data logging system to identify operational faults and behavioural anomalies effectively.

PLAT: An Automated Fault and Behavioural Anomaly Detection Tool for PLC Controlled Manufacturing Systems

Directory of Open Access Journals (Sweden)

Arup Ghosh

2016-01-01

Full Text Available Operational faults and behavioural anomalies associated with PLC control processes take place often in a manufacturing system. Real time identification of these operational faults and behavioural anomalies is necessary in the manufacturing industry. In this paper, we present an automated tool, called PLC Log-Data Analysis Tool (PLAT that can detect them by using log-data records of the PLC signals. PLAT automatically creates a nominal model of the PLC control process and employs a novel hash table based indexing and searching scheme to satisfy those purposes. Our experiments show that PLAT is significantly fast, provides real time identification of operational faults and behavioural anomalies, and can execute within a small memory footprint. In addition, PLAT can easily handle a large manufacturing system with a reasonable computing configuration and can be installed in parallel to the data logging system to identify operational faults and behavioural anomalies effectively.

Reliability Analysis of Safety Grade PLC(POSAFE-Q) for Nuclear Power Plants

International Nuclear Information System (INIS)

Kim, J. Y.; Lyou, J.; Lee, D. Y.; Choi, J. G.; Park, W. M.

2006-01-01

The Part Count Method of the military standard MILHDK- 217F has been used for the reliability prediction of the nuclear field. This handbook determines the Programmable Logic Controller (PLC) failure rate by summing the failure rates of the individual component included in the PLC. Normally it is easily predictable that the components added for the fault detection improve the reliability of the PLC. But the application of this handbook is estimated with poor reliability because of the increased component number for the fault detection. To compensate this discrepancy, the quantitative reliability analysis method is suggested using the functional separation model in this paper. And it is applied to the Reactor Protection System (RPS) being developed in Korea to identify any design weak points from a safety point of view

The Research about Embedded Soft PLC Running System Based on ARM

Directory of Open Access Journals (Sweden)

Fang Ding

2014-09-01

Full Text Available The paper discusses the overall construction and operational principle of soft PLC. Considering the real time request of the system, we use Linux+RTAI dual core system as software platform. In this platform, the implementation method of soft PLC operational system is introduced. Especially, the design of instruction analysis module is emphasized. On the basis of energy flow concept, the logic algorithm is established, aligning to the left bus. Finally, the system is tested to evaluate the desired control behavior.

Development of Safety Grade PLC (POSAFE-Q) and Performance Test Results

International Nuclear Information System (INIS)

Kim, Chang Hwoi; Park, Won Man; Choi, Jong Gyun; Lee, Dong Young; No, Young Hun; Song, Seung Hwan

2006-01-01

The safety grade PLC (POSAFE-Q) is being developed in the Korea Nuclear Instrumentation and Control System (KNICS) R and D project. The PLC satisfies Safety Class 1E, Quality Class 1, and Seismic Category I. The software such as the RTOS and firmware are being developed according to the safety critical software life cycle. Especially, the formal method is applied to design the SRS (Software Requirement Spec.) and the SDS (Software Design Specification.) to be error-free. The POSAFE-Q has several modules such as processor module, input and output modules, communication modules, redundant processor module, redundant power modules, etc,. To verify the function and performance, several tests such as CT, IT and ST were performed. And also, the equipment qualification test for environment, EMI and EMC, and seismic ware performed. All tests are satisfied with the requirements and specification for safety grade PLC, and the criteria for safety system in nuclear power plants

Development of Safety Grade PLC (POSAFE-Q) and Performance Test Results

Energy Technology Data Exchange (ETDEWEB)

Kim, Chang Hwoi; Park, Won Man; Choi, Jong Gyun; Lee, Dong Young [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of); No, Young Hun; Song, Seung Hwan [POSCON, Seoul (Korea, Republic of)

2006-07-01

The safety grade PLC (POSAFE-Q) is being developed in the Korea Nuclear Instrumentation and Control System (KNICS) R and D project. The PLC satisfies Safety Class 1E, Quality Class 1, and Seismic Category I. The software such as the RTOS and firmware are being developed according to the safety critical software life cycle. Especially, the formal method is applied to design the SRS (Software Requirement Spec.) and the SDS (Software Design Specification.) to be error-free. The POSAFE-Q has several modules such as processor module, input and output modules, communication modules, redundant processor module, redundant power modules, etc,. To verify the function and performance, several tests such as CT, IT and ST were performed. And also, the equipment qualification test for environment, EMI and EMC, and seismic ware performed. All tests are satisfied with the requirements and specification for safety grade PLC, and the criteria for safety system in nuclear power plants.

Praksisnær skoleudvikling og PLC som strategisk udviklingsaktør

DEFF Research Database (Denmark)

Christensen, Ole; Bach, Anna

2017-01-01

I det følgende belyses, hvorledes PLC kan udvikles som strategisk udviklingsaktør og understøtte praksisnær skoleudvikling. Vi ønsker at belyse hvorledes Bekendtgørelsens overordnede intentioner kan få retning og rammer og dermed medvirke til, at hele det pædagogiske personale indgår i skoleudvik......I det følgende belyses, hvorledes PLC kan udvikles som strategisk udviklingsaktør og understøtte praksisnær skoleudvikling. Vi ønsker at belyse hvorledes Bekendtgørelsens overordnede intentioner kan få retning og rammer og dermed medvirke til, at hele det pædagogiske personale indgår i...... skoleudviklingen. I den forbindelse er det vores erfaring, at PLC kan spille en central rolle i forbindelse med den praksisnære og lokale skoleudvikling. Erfaringerne bygger især på udviklings- og forskningsprojekter fra kommunerne Kolding, København, Roskilde og Tårnby. Der har været tale om praksisnære...

Slovak Electric, Plc., 1998

International Nuclear Information System (INIS)

1997-06-01

Slovenske elektrarne, a.s. (Slovak Electric, Plc., abbrevation 'SE, a.s.') is the Slovak electricity generating utility, incorporated on November 1, 1994 as one of new companies formed from substantially all of the assets and a legal successor of Slovensky energeticky podnik, s.p., founded on January 1, 1969 in the form of SEP group. From its predecessor, Slovak Electric, Plc., took over generation of power, operation of 220 kV and 400 kV power system, transit, import, export, and sale of electricity. It is also involved in generation, distribution, and sale of heat. At present, SE's share of electriciry sales in the Slovak Republic is 88.47%. Electricity is delivered to three regional distribution companies and directly to several major industrial enterprises. SE, a.s. operates one nuclear power station, three thermal power plants, and 30 hydro power plants. The second nuclear power plant is under construction (state up tu June 1997) and SE is participating in the construction of two hydro power plants and one combined cycle power plant. The efforts of SE, s.a. focus on the generation of power and heat with minimal environmental impacts. Ecology is given priority in the SE, a.s. development programmes. SE's mission is to permanently satisfy customers' needs, for an acceptable price and with minimal environmental impact. On this CD ROM next chapters are presented: (1) The structure of the Company; (2) Production units; (3) The economic power of the Company; (4) The operation culture; (5) The strategic plans of the Company

Applying Hamming Code to Memory System of Safety Grade PLC (POSAFE-Q) Processor Module

Energy Technology Data Exchange (ETDEWEB)

Kim, Taehee; Hwang, Sungjae; Park, Gangmin [POSCO Nuclear Technology, Seoul (Korea, Republic of)

2013-05-15

If some errors such as inverted bits occur in the memory, instructions and data will be corrupted. As a result, the PLC may execute the wrong instructions or refer to the wrong data. Hamming Code can be considered as the solution for mitigating this mis operation. In this paper, we apply hamming Code, then, we inspect whether hamming code is suitable for to the memory system of the processor module. In this paper, we applied hamming code to existing safety grade PLC (POSAFE-Q). Inspection data are collected and they will be referred for improving the PLC in terms of the soundness. In our future work, we will try to improve time delay caused by hamming calculation. It will include CPLD optimization and memory architecture or parts alteration. In addition to these hamming code-based works, we will explore any methodologies such as mirroring for the soundness of safety grade PLC. Hamming code-based works can correct bit errors, but they have limitation in multi bits errors.

A Research on Seamless Platform Change of Reactor Protection System From PLC to FPGA

Energy Technology Data Exchange (ETDEWEB)

Yoo, Junbeom; Lee, Jonghoon [Konkuk Univ., Seoul (Korea, Republic of); Lee, Jangsoo [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

2013-08-15

The PLC (Programmable Logic Controller) has been widely used to implement real-time controllers in nuclear RPSs (Reactor Protection Systems). Increasing complexity and maintenance cost, however, are now demanding more powerful and cost-effective implementation such as FPGA (Field-Programmable Gate Array). Abandoning all experience and knowledge accumulated over the decades and starting an all-new development approach is too risky for such safety-critical systems. This paper proposes an RPS software development process with a platform change from PLC to FPGA, while retaining all outputs from the established development. This paper transforms FBD designs of the PLC-based software development into a behaviorally-equivalent Verilog program, which is a starting point of a typical FPGA-based hardware development. We expect that the proposed software development process can bridge the gap between two software developing approaches with different platforms, such as PLC and FPGA. This paper also demonstrates its effectiveness using an example of a prototype version of a real-world RPS in Korea.

A Research on Seamless Platform Change of Reactor Protection System From PLC to FPGA

International Nuclear Information System (INIS)

Yoo, Junbeom; Lee, Jonghoon; Lee, Jangsoo

2013-01-01

The PLC (Programmable Logic Controller) has been widely used to implement real-time controllers in nuclear RPSs (Reactor Protection Systems). Increasing complexity and maintenance cost, however, are now demanding more powerful and cost-effective implementation such as FPGA (Field-Programmable Gate Array). Abandoning all experience and knowledge accumulated over the decades and starting an all-new development approach is too risky for such safety-critical systems. This paper proposes an RPS software development process with a platform change from PLC to FPGA, while retaining all outputs from the established development. This paper transforms FBD designs of the PLC-based software development into a behaviorally-equivalent Verilog program, which is a starting point of a typical FPGA-based hardware development. We expect that the proposed software development process can bridge the gap between two software developing approaches with different platforms, such as PLC and FPGA. This paper also demonstrates its effectiveness using an example of a prototype version of a real-world RPS in Korea

Applying Hamming Code to Memory System of Safety Grade PLC (POSAFE-Q) Processor Module

International Nuclear Information System (INIS)

Kim, Taehee; Hwang, Sungjae; Park, Gangmin

2013-01-01

If some errors such as inverted bits occur in the memory, instructions and data will be corrupted. As a result, the PLC may execute the wrong instructions or refer to the wrong data. Hamming Code can be considered as the solution for mitigating this mis operation. In this paper, we apply hamming Code, then, we inspect whether hamming code is suitable for to the memory system of the processor module. In this paper, we applied hamming code to existing safety grade PLC (POSAFE-Q). Inspection data are collected and they will be referred for improving the PLC in terms of the soundness. In our future work, we will try to improve time delay caused by hamming calculation. It will include CPLD optimization and memory architecture or parts alteration. In addition to these hamming code-based works, we will explore any methodologies such as mirroring for the soundness of safety grade PLC. Hamming code-based works can correct bit errors, but they have limitation in multi bits errors

A RESEARCH ON SEAMLESS PLATFORM CHANGE OF REACTOR PROTECTION SYSTEM FROM PLC TO FPGA

Directory of Open Access Journals (Sweden)

JUNBEOM YOO

2013-08-01

Full Text Available The PLC (Programmable Logic Controller has been widely used to implement real-time controllers in nuclear RPSs (Reactor Protection Systems. Increasing complexity and maintenance cost, however, are now demanding more powerful and cost-effective implementation such as FPGA (Field-Programmable Gate Array. Abandoning all experience and knowledge accumulated over the decades and starting an all-new development approach is too risky for such safety-critical systems. This paper proposes an RPS software development process with a platform change from PLC to FPGA, while retaining all outputs from the established development. This paper transforms FBD designs of the PLC-based software development into a behaviorally-equivalent Verilog program, which is a starting point of a typical FPGA-based hardware development. We expect that the proposed software development process can bridge the gap between two software developing approaches with different platforms, such as PLC and FPGA. This paper also demonstrates its effectiveness using an example of a prototype version of a real-world RPS in Korea.

A Remote PLC Laboratory (RLab) for Distance Practical Work of Industrial Automation

Science.gov (United States)

Haritman, E.; Somantri, Y.; Wahyudin, D.; Mulyana, E.

2018-02-01

A laboratory is an essential equipment for engineering students to do a useful practical work. Therefore, universities should provide an adequate facility for practical work. On the other hand, industrial automation laboratory would offer students beneficial experience by using various educational PLC kits. This paper describes the development of Web-based Programmable Logic Controller (PLC) remote laboratory called RLab. It provides an environment for learners to study PLC application to control the level of the non-interacting tank. The RLab architecture is based on a Moodle and Remote Desktop, which also manages the booking system of the schedule of practical work in the laboratory. The RLab equipped by USB cameras providing a real-time view of PLC environment. To provide a secured system, the RLab combines Moodle and Remote Desktop application for the authentication system and management of remote users. Moodle will send PartnerID and password to connect to TeamViewer. It has been examined that the laboratory requirement, time and flexibility restrictions constitute a significant obstacle facing traditional students desiring to finish the course. A remote access laboratory can be eliminating time and flexibility restrictions. The preliminary study of RLab usability proved that such system is adequate to give the learners a distance practical work environment.

Data communication between Panasonic PLC and PC using SerialPort control in C#.NET environment

Science.gov (United States)

Gao, Ting; Gan, Xiaochuan; Ma, Liqun

2015-02-01

With the gradual promotion of Microsoft.NET platform, C# as an object-oriented programming language based on the platform has been widely used. Therefore, more attention is concentrated on how to achieve the communication between Panasonic PLC and PC efficiently and fast in C#.NET environment. In this paper, a method of using SerialPort control which could be used for achieving communication between PLC and PC is introduced. Meanwhile, the reason of abnormal thread when displayed the receiving data in form is analyzed and the programming method to solve the problem of thread safety is designed. Achieving the communication of Panasonic PLC and PC in C#.NET environment can give full play to the advantages of the .NET framework. It is practical, easy communication, high reliability and can combine with other measurement and calibration procedures effectively and conveniently. Configuration software is expensive and can only communicate with PLC separately, but these shortcomings can be solved in C#.NET environment. A well-designed user interface realized real-time monitoring of PLC parameters and achieved management and control integration. The experiment show that this method of data transfer is accurate and the program' running is stable.

Equity valuation of Tesco Plc.

OpenAIRE

Schmitz, Clemens

2015-01-01

This dissertation aimed to value the British retailer Tesco Plc. The objective was to determine a target price for the company and as a consequence determine a buy or sell recommendation when comparing it to the current market price. After the state of the art of literature regarding equity valuation has been assessed, the retail industry as well as Tesco have been analysed in more detail. Based on this, the retail business of Tesco has been valued using the Adjusted Present Va...

Development of the safety PLC for plant protection system

Energy Technology Data Exchange (ETDEWEB)

Kim, Chang Hwoi; Lee, Dong Young [Korea Atomic Energy Research Institute, Taejeon (Korea, Republic of)

2005-11-15

The safety PLC (POSAFE-Q) is developing in the Korea Nuclear Instrumentation and Control System (KNICS) R and D project. The PLC satisfies Safety Class 1E, Quality Class 1, and Seismic Category I. The software such as RTOS and firmware are developed according to safety critical software life cycle. Especially, the formal method is applied to design SRS (Software Requirement Spec.) and SDS (Software Design Specification.) for error-free. The developed software according to software life cycle is verified by independent software V and V team. The overall response time from an input to the outputs shall be 50ms or less. The prototype for the POSAFE-Q was developed and functional testing and equipment qualification tests have been underway.

An Architecture Of Plc Ls Xbc-Dr30e Based Clean Water Controlling System

Directory of Open Access Journals (Sweden)

M.Jusuf Jamanawar Purba

2017-12-01

Full Text Available In the industrial field the good controlling system is definitely required to improve the working efficiency of a system. The type of controller PLC Programmable Logic Controller used in Clean Water Controlling System in Electrical Workshop with PLC LS XBC-DR30E. Furthermore such system consistently uses two types of component they are Relay and Timer. The clean water pump control aims to pump the water in well A to storage tank B. The pump will work when water-contained well A is marked by sensor level well A on and the water inside storage tank B is under level 3. When the water inside storage tank B is under level 2 both pumps M1 and M2 will work to fill storage tank B. on the contrary when storage tank B is above level 2 only one pump M1 works until the tank reach level 3. In addition when storage tank B is above level 3 both pumps will stop working. However along with the advancement of recent technology the above system can be controlled by using PLC Programmable Logic Controller. Therefore it is possible to apply the controlling method of PLC as the semester Vs practical module. Based on the trial performed the PLC-based clean water system is well-functioned as the working description compiled before the operation of the tool.

PLC based control system and maintenance activities at NCAR, Bilaspur

International Nuclear Information System (INIS)

Dewangan, Jaidev; Trivedi, T.; Patel, Shiv P.; Malik, C.; Kumar, Rakesh; Gupta, Santosh Kumar; Bajpai, P.K.

2015-01-01

A 3.0 MV high current low energy Pelletron Accelerator facility (Model 9SDH-4, NEC, USA) with TORUIS (ion source for H + and He 2+ beam current H + ion ∼ 50μA @ 6 MeV, He 2+ at ∼ 10μA) and SNICS-II ion source for heavy ions has been commissioned as 'National Centre for Accelerator Based Research' in the Department of Pure and Applied Physics, Guru Ghasidas Vishwavidyalaya. In this paper, we detail out the control system developed and implemented at NCAR. The basic idea of controlling the machine is by providing the output signal through PLC to ACPC of accelerator using user interface points provided by the manufacturer. The PLC based system generates output signal once it receives the feedback signals from search and secure switches, door lock switches and scram switches interlocked with PLC. The output is controlled by ladder logic and is activated only when all the radiation monitors are in healthy state and outside radiations monitor having low radiation level. The details of control system and maintenance activities will be discussed in the paper

Enterprise network with software Asterisk PBX based on the PLC technology

Directory of Open Access Journals (Sweden)

Michal Maar

2017-01-01

Full Text Available This article presents the software Asterisk PBX solution design in enterprise PLC network (Power Line Communication. The description of the installation and configuration of software Asterisk PBX is involved in the design. The secure interconnection of two enterprise PLC network is implemented via the telecommunication tunnel with security grant using the Cisco routers. The connection between two Asterisk PBXs is designed in context of the establishment of the tunnel. The subject of the article is also cross/connection of exchanges Asterisk PBX and hardware PBX - IP Panasonic PBX K-NS500.

β Absorption dust concentration monitor system based on the PLC

International Nuclear Information System (INIS)

Lian Dexing; Zhou Xiuliang; Liu Liyan; Li Zonglun; Cao Guanghui; Mao Wanchong

2011-01-01

To provide an on-line automatic system for dust concentration monitor is the purpose of this design.The system based on β-ray-absorption-method,employing PLC (Programmable Logic Controller) control technology, is presented. The user input parameter setup,the data acquisition and processing, and the communication with computer can be effectively regulated using the advanced PLC control technology. It has the features of high reliability, easy operation and easy maintenance, and the ability to monitor remotely. Measurement range: 0.1-1500 mg/m 3 , Accuracy: ±9%. The structure,working principle, electric circuit and software development of the system are introduced in detail. (authors)

Bringing Automated Model Checking to PLC Program Development - A CERN Case Study

CERN Document Server

Fernandez Adiego, B; Tournier, J-C; Blanco Vinuela, E; Gonzalez Suarez, V M

2014-01-01

Verification of critical software is a high priority but a challenging task for industrial control systems. Model checking appears to be an appropriate approach for this purpose. However, this technique is not widely used in industry yet, due to some obstacles. The main obstacles encountered when trying to apply formal verification techniques at industrial installations are the difficulty of creating models out of PLC programs and defining formally the specification requirements. In addition, models produced out of real-life programs have a huge state space, thus preventing the verification due to performance issues. Our work at CERN (European Organization for Nuclear Research) focuses on developing efficient automatic verification methods for industrial critical installations based on PLC (Programmable Logic Controller) control systems. In this paper, we present a tool generating automatically formal models out of PLC code. The tool implements a general methodology which can support several input languages, ...

Bringing Automated Formal Verification to PLC Program Development

CERN Document Server

Fernández Adiego, Borja; Blanco Viñuela, Enrique

Automation is the field of engineering that deals with the development of control systems for operating systems such as industrial processes, railways, machinery or aircraft without human intervention. In most of the cases, a failure in these control systems can cause a disaster in terms of economic losses, environmental damages or human losses. For that reason, providing safe, reliable and robust control systems is a first priority goal for control engineers. Ideally, control engineers should be able to guarantee that both software and hardware fulfill the design requirements. This is an enormous challenge in which industry and academia have been working and making progresses in the last decades. This thesis focuses on one particular type of control systems that operates industrial processes, the PLC (Programmable Logic Controller) - based control systems. Moreover it targets one of the main challenges for these systems, guaranteeing that PLC programs are compliant with their specifications. Traditionally ...

76 FR 52288 - Airworthiness Directives; Rolls-Royce plc (RR) Trent 800 Series Turbofan Engines

Science.gov (United States)

2011-08-22

...-0836; Directorate Identifier 2010-NE-38-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc (RR... plc, P.O. Box 31, Derby, DE24 8BJ, United Kingdom: telephone 44 (0) 1332 242424; fax 44 (0) 1332... based on those comments. We will post all comments we receive, without change, to http://www.regulations...

A PLC generic requirements and specification for safety-related applications in nuclear power plants

International Nuclear Information System (INIS)

Han, Jea Bok; Lee, C. K.; Lee, D. Y.

2001-12-01

This report presents the requirements and specification to be applied to the generic qualification of programmable Logic Controller(PLC), which is being developed as part of the KNICS project, 'Development of the Digital Reactor Safety Systems' of which purpose is the application to safety-related instrumentation and control systems in nuclear power plants. This report defines the essential and critical characteristics that shall be included as part of a PLC design for safety-related application. The characteristics include performance, reliability, accuracy, the overall response time from an input to the PLC exceeding it trip condition to the resulting outputs, and the specification of processors and memories in digital controller. It also specifies the quality assurance process for software development, dealing with executive software, firmware, application software tools for developing the application software, and human machine interface(HMI). In addition, this report reviews the published standards and guidelines that are required for the PLC development and the quality assurance processes such as environment requirements, seismic withstand requirements, EMI/RFI withstand requirements, and isolation test

Technical considerations for the development of an engineering safety features control system with PLC

International Nuclear Information System (INIS)

Lee, C. K.; Kim, C. H.; Han, J. B.; Kim, H.; Lee, S. S.

2002-01-01

Technical considerations are summarized for the development of an ESFCS(Engineered Safety Features Control System) with PLC (Programmable Logic Controller). The ESFCS is required for the mitigation of plant accident conditions and therefore developed in conformance with the design requirements applied to the safety critical system. The design of ESFCS primarily considered its safety, and the system has an architecture that will be able to minimize spurious actuation. The PLC based functional distribution and redundant design features are adopted, and the fieldbus is applied in the communication of information and control signals between PLC processors. It is expected that the ESFCS will have several advanced design features compared with the conventional systems supplied by foreign vendors

The YvfTU Two-component System is involved in plcR expression in Bacillus cereus

NARCIS (Netherlands)

Brillard, Julien; Susanna, Kim; Michaud, Caroline; Dargaignaratz, Claire; Gohar, Michel; Nielsen-Leroux, Christina; Ramarao, Nalini; Kolsto, Anne-Brit; Nguyen-The, Christophe; Lereclus, Didier; Broussolle, Veronique

2008-01-01

Background: Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative

Amersham International plc. Report and Accounts 1995

International Nuclear Information System (INIS)

1995-01-01

The Annual Report and Accounts for Amersham International plc for the year 1995 are presented. This leading health science company develops products, services and technologies based on its expertise in labelling and detection at the molecular level. Recent work in the fields of life science research, nuclear medicine and industrial quality and safety assurance are described. (UK)

Csseverin inhibits apoptosis through mitochondria-mediated pathways triggered by Ca2 + dyshomeostasis in hepatocarcinoma PLC cells.

Directory of Open Access Journals (Sweden)

Mengchen Shi

2017-11-01

Full Text Available Numerous experimental and epidemiological studies have demonstrated a link between Clonorchis sinensis (C. sinensis infestation and cholangiocarcinoma (CCA as well as hepatocellular carcinoma (HCC. The underlying molecular mechanism involved in the malignancy of CCA and HCC has not yet been addressed. Csseverin, a component of the excretory/secretory products of C. sinensis (CsESPs, was confirmed to cause obvious apoptotic inhibition in the human HCC cell line PLC. However, the antiapoptotic mechanism is unclear. In the present study, we investigated the cellular features of the antiapoptotic mechanism upon transfection of the Csseverin gene.In the present study, we evaluated the effects of Csseverin gene overexpression on the apoptosis of PLC cells using an Annexin PE/7-AAD assay. Western blotting was applied to quantify the activation of caspase-3 and caspase-9, the mitochondrial translocation of Bax and the release of Cyt c upon Csseverin overexpression in PLC cells. Laser scanning confocal microscopy was used to analyze the changes of intracellular calcium. Fluorescence assay and immunofluorescence assays were performed to observe the changes of the mitochondrial permeability transition pore (MPTP.The overexpression of Csseverin in PLC cells showed apoptosis resistance after the induction of apoptosis. Additionally, the activation of caspase-3 and caspase-9 was specifically weakened in Csseverin overexpression PLC cells. The overexpression of Csseverin reduced the increase in intracellular free Ca2+, thereby inhibiting MPTP opening in PLC cells. Moreover, Bax mitochondrial translocation and the subsequent release of Cyt c were downregulated in apoptotic Csseverin overexpression PLC cells.The present findings suggest that Csseverin, a component of CsESPs, confers protection from human HCC cell apoptosis via the inactivation of membranous Ca2+ channels. Csseverin might be involved in the process of HCC through C. sinensis infestation in

An advanced lab frame PC-PLC regime for a power supply control

International Nuclear Information System (INIS)

Abdel-Bary, M

2010-01-01

A lab frame automatic control system (LFACS) based on a programmable logic controller (PLC) is designed, developed and tested in the laboratory. The LFACS is designed to control and monitor one DC power supply with output current accuracy 0.001 amps, one automatic valve. Also the LFACS will monitor one full range vacuum measurement and one water leak detector. The control system is built based on the internationally standard programmable logic controller (PLC) from Siemens. Two software's have been used; firstly SIMATIC S 7 lite has been used to build the control program and safety interlocks, secondly winCC flexible (runs in a PC computer and communicates with the PLC by multi point interface PC adapter (MPI) has been used to build the user-machine interface . This lab frame control system will be generalized to a global control system to control the MGC-20 cyclotron. The lab tests showed a reliable and flexible control system.

76 FR 24796 - Airworthiness Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines

Science.gov (United States)

2011-05-03

... Airworthiness Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines AGENCY: Federal Aviation.... Request To Revise the Compliance Times Four commenters, American Airlines, Delta Airlines, Rolls-Royce plc... SNPRM were developed to minimize the risk of uncontained disc failure, based on the age of the parts in...

Session 1984-1985. Radioactive waste. Minutes of evidence, Monday 13 May 1985. British Nuclear Fuels plc

Energy Technology Data Exchange (ETDEWEB)

1985-01-01

The Environment Select Committee of the House of Commons received a memorandum from British Nuclear Fuels plc on the treatment and preparation for disposal of radioactive wastes, under the headings: introduction; waste categories; waste management policy; waste arisings; waste treatment plans; appendix I - British Nuclear Fuels plc; appendix II - the nuclear fuel cycle for Magnox, AGR and LWR reactors; appendix III - control of liquid radioactive discharges from Sellafield and their environmental impact. Representatives of BNF plc were examined on the subject of the memorandum and the minutes of evidence are recorded.

Session 1984-85. Radioactive waste. Minutes of evidence, Monday 13 May 1985. British Nuclear Fuels plc

International Nuclear Information System (INIS)

1985-01-01

The Environment Select Committee of the House of Commons received a memorandum from British Nuclear Fuels plc on the treatment and preparation for disposal of radioactive wastes, under the headings: introduction; waste categories; waste management policy; waste arisings; waste treatment plans; appendix I - British Nuclear Fuels plc; appendix II - the nuclear fuel cycle for Magnox, AGR and LWR reactors; appendix III - control of liquid radioactive discharges from Sellafield and their environmental impact. Representatives of BNF plc were examined on the subject of the memorandum and the minutes of evidence are recorded. (U.K.)

70Z/3 Cbl induces PLC gamma 1 activation in T lymphocytes via an alternate Lat- and Slp-76-independent signaling mechanism.

Science.gov (United States)

Graham, Laurie J; Verí, Maria-Concetta; DeBell, Karen E; Noviello, Cristiana; Rawat, Rashmi; Jen, Sandy; Bonvini, Ezio; Rellahan, Barbara

2003-04-24

The oncoprotein 70Z/3 Cbl signals in an autonomous fashion or through blockade of endogenous c-Cbl, a negative regulator of signaling. The mechanism of 70Z/3 Cbl-induced signaling was investigated by comparing the molecular requirements for 70Z/3 Cbl- and TCR-induced phospholipase C gamma 1 (PLC gamma 1) activation. 70Z/3 Cbl-induced PLC gamma 1 tyrosine phosphorylation required, in addition to the PLC gamma 1 N-terminal SH2 domain, the C-terminal SH2 and SH3 domains that were dispensable for TCR-induced phosphorylation. Deletion of the leucine zipper of 70Z/3 Cbl did not eliminate 70Z/3 Cbl-induced PLC gamma 1 phosphorylation, suggesting that blockage of c-Cbl via dimerization with 70Z/3 Cbl cannot fully explain 70Z/3 Cbl activating characteristics. The complete elimination of PLC gamma 1 phosphorylation required deleting the SH3 domain-binding region of 70Z/3 Cbl, consistent with 70Z/3 Cbl binding the PLC gamma 1 SH3 domain. 70Z/3 Cbl-induced PLC gamma 1 phosphorylation required Zap-70, as for the TCR, and the tyrosine kinase binding domain of 70Z/3 Cbl, which binds Zap-70, but did not require PLC gamma 1 binding to Lat, a crucial interaction in TCR-induced PLC gamma 1 phosphorylation. Furthermore, 70Z/3 Cbl-induced activation of NFAT, a PLC gamma 1/Ca(2+)-dependent transcriptional event, required Zap-70, but was independent of Slp-76, an adapter required for TCR-induced NFAT activation. These results suggest that 70Z/3 Cbl and PLC gamma 1 form a TCR-, Lat- and Slp-76-independent complex that leads to PLC gamma 1 phosphorylation and activation.

Application of PLC technology in measurement of beam profile on 100 MeV accelerator

International Nuclear Information System (INIS)

Yu Luyang; Chinese Academy of Sciences, Beijing; Chen Yongzhong; Chen Yongzhong; Liu Dekang; Chinese Academy of Sciences, Beijing

2005-01-01

A comprehensive introduction is given to the real-time measuring method, which is based on the Programmable Logic Controller (PLC) technology and can measure intensity and profile of the beam by a scintillator screen. The whole system has many advantages, such as good reliability, high precision, intuitional measurement, etc. due to the use of the PLC and Labview software. (authors)

PowerGen plc report and accounts 1994

International Nuclear Information System (INIS)

1994-01-01

The annual report and accounts of PowerGen plc for the year 1994 are presented. Financial highlights are quoted, followed by the Chairman's statement, reviews by the Chief Executive and Financial Directors, reports by the Auditors and Directors, balance sheets and details of the consolidated profit and loss account and principal accounting policies. A four year summary and shareholder information are included. (UK)

75 FR 61114 - Airworthiness Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines

Science.gov (United States)

2010-10-04

... Airworthiness Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines AGENCY: Federal Aviation.... Fax: (202) 493-2251. Contact Rolls-Royce plc, P.O. Box 31, Derby, England, DE248BJ; telephone: 011-44... AD. We will consider all comments received by the closing date and may amend this proposed AD based...

75 FR 264 - Airworthiness Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines

Science.gov (United States)

2010-01-05

...-1004; Directorate Identifier 2009-NE-36-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc.... Contact Rolls-Royce plc, P.O. Box 31, Derby, England; telephone: 011-44-1332-249428; fax: 011-44-1332... AD. We will consider all comments received by the closing date and may amend this proposed AD based...

77 FR 1009 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-524 Series Turbofan Engines

Science.gov (United States)

2012-01-09

... Airworthiness Directives; Rolls-Royce plc (RR) RB211-524 Series Turbofan Engines AGENCY: Federal Aviation... 5, 2005). ADDRESSES: For service information identified in this AD, contact Rolls-Royce plc, P.O...,000. Based on these figures, we estimate the total cost of the AD to U.S. operators to be $4,206,960...

Five Year Retrospective Study of the Financial Situation of Northern Foods Plc., United Kingdom

Directory of Open Access Journals (Sweden)

Louie DACOSTA

2016-09-01

Full Text Available This study was conducted as a retrospective analysis of Northern Foods Plc., once a major player in FTSE 350 Food Sector, to evaluate its financial situation over a five year period. The ex post factor research design was used for this study. Annual reports and databases on Northern Foods Plc., and Associated British Foods Plc., were used to perform a series of ratio analyses. The results revealed that Northern Foods Plc.’s performance has been declining as evidenced in the profitability ratios calculated. Also, financial strength was weak and working capital has not been effectively managed, hence affecting its cash and profit generation potentials. The company was limited in its ability to grow and expand as it needed to regularly fund its pension deficit, and finance its high levels of debt. The study concludes that Northern Foods was not in a very strong financial position, yet it was not making the required investments to improve, hence its takeover though this paper will not rule out non-financial issues. Furthermore, the study prescribed five generic points to improve the financial health of any organisation.

Application of PLC to dynamic control system for liquid He cryogenic pumping facility on JT-60U NBI system

International Nuclear Information System (INIS)

Honda, A.; Okano, F.; Ooshima, K.; Akino, N.; Kikuchi, K.; Tanai, Y.; Takenouchi, T.; Numazawa, S.; Ikeda, Y.

2008-01-01

The control system of the cryogenic facility in the JT-60 NBI system has been replaced by employing the PLC (Programmable Logic Controller) and SCADA (Supervisory Control And Data Acquisition) system. The original control system was constructed about 20 years ago by specifying the DCS (Distributed Control System) computer to deal with ∼400 feedback loops. Recently, troubles on this control system have increased due to its age-induced deterioration. To maintain the high reliability of the cryogenic facility, a new control system has been planned with the PLC and SCADA systems. Their attractive features include high market availability and cost-effectiveness, however, the use of PLC for such a large facility with ∼400 feedback loops has not been established because of insufficient processing capability of the early PLC. Meanwhile, the recent progress in the PLC enables to use the FBD (function block diagram) programming language for 500 function blocks. By optimizing the function blocks and connecting them in the FBD language, the feedback loops have been successfully replaced from DCS to PLC without a software developer. Moreover, an oscillation of the liquid He level, which often occurs during the cooldown mode of the cryopumps, can be automatically stabilized by easily adding a new process program in the PLC. At present, the new control system has worked well

A PLC platform-independent structural analysis on FBD programs for digital reactor protection systems

International Nuclear Information System (INIS)

Jung, Sejin; Yoo, Junbeom; Lee, Young-Jun

2017-01-01

Highlights: • FBD has been widely used to implement safety-critical software for PLC-based systems. • The safety-critical software should be developed strictly with safety programming guidelines. • There are no argued rules that have specific links to higher guidelines NUREG/CR-6463 PLC platform-independently. • This paper proposes a set of rules on the structure of FBD programs with providing specific links to higher guidelines. • This paper also provides CASE tool ‘FBD Checker’ for analyzing the structure of FBD. - Abstract: FBD (function block diagram) has been widely used to implement safety-critical software for PLC (programmable logic controller)-based digital nuclear reactor protection systems. The software should be developed strictly in accordance with safety programming guidelines such as NUREG/CR-6463. Software engineering tools of PLC vendors enable us to present structural analyses using FBD programs, but specific rules pertaining to the guidelines are enclosed within the commercial tools, and specific links to the guidelines are not clearly communicated. This paper proposes a set of rules on the structure of FBD programs in accordance with guidelines, and we develop an automatic analysis tool for FBD programs written in the PLCopen TC6 format. With the proposed tool, any FBD program that is transformed into an open format can be analyzed the PLC platform-independently. We consider a case study on FBD programs obtained from a preliminary version of a Korean nuclear power plant, and we demonstrate the effectiveness and potential of the proposed rules and analysis tool.

Restructuring of SE, Plc - from a functionally managed company to a process managed commercial-production company

International Nuclear Information System (INIS)

Ravasz, V.

2004-01-01

The purpose of this presentation is to inform the participants about present changes currently occurred within Slovenske elektrarne, a. s. (SE, Plc). Changes are related to the liberalisation of the electricity market and expected accession of Slovakia to EU. To support its competitiveness, the SE, Plc became a customer-oriented company, prepared to the access of strategic investor. The paper includes the basic information about centralisation and project of Restructuring of SE, Plc., which has started in December 2003. The aim of Restructuring is the change from functionally managed company to a process managed commercial-production company

77 FR 20987 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2012-04-09

... Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT. ACTION... the Federal Register. That AD applies to RB211-Trent 800 series turbofan engines. The last comment...

75 FR 61361 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-535 Series Turbofan Engines

Science.gov (United States)

2010-10-05

...-0994; Directorate Identifier 2009-NE-39-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc (RR...-Royce plc., P.O. Box 31, Derby, DE24 8BJ, United Kingdom; Telephone: 011 44 1332 242424, Fax: 011 44... based on those comments. We will post all comments we receive, without change, to http://www.regulations...

76 FR 65997 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-535 Series Turbofan Engines

Science.gov (United States)

2011-10-25

...-0994; Directorate Identifier 2009-NE-39-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc (RR... Friday, except Federal holidays. For service information identified in this AD, contact Rolls-Royce plc.... Based on these figures, we estimate the cost of this proposed AD on U.S. operators to be $1,499,400. FAA...

76 FR 2605 - Airworthiness Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines

Science.gov (United States)

2011-01-14

... Airworthiness Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines AGENCY: Federal Aviation... holidays. Fax: (202) 493-2251. Contact Rolls-Royce plc, P.O. Box 31, DERBY, DE24 8BJ, UK; telephone 44 (0... AD. We will consider all comments received by the closing date and may amend this proposed AD based...

Event notification system with a PLC

International Nuclear Information System (INIS)

Kawase, M.; Yoshikawa, Hiroshi; Sakaki, Hironao; Takahashi, Hiroki; Sako, Hiroyuki; Kamiya, Junichiro; Takayanagi, Tomohiro

2004-01-01

When an interlock occurs in the equipment, it is required to notify the upper rank control system of the Interlock and receive information for apparatus information in the upper rank control system as at high speed as possible. In the apparatus using FA-M3, it can respond to this by using the notice function of an event. This report shows the event notification system with a PLC based Kicker electromagnet power supply for 3GeV RCS. (author)

PLC-based LP₁₁ mode rotator for mode-division multiplexing transmission.

Science.gov (United States)

Saitoh, Kunimasa; Uematsu, Takui; Hanzawa, Nobutomo; Ishizaka, Yuhei; Masumoto, Kohei; Sakamoto, Taiji; Matsui, Takashi; Tsujikawa, Kyozo; Yamamoto, Fumihiko

2014-08-11

A PLC-based LP11 mode rotator is proposed. The proposed mode rotator is composed of a waveguide with a trench that provides asymmetry of the waveguide. Numerical simulations show that converting LP11a (LP11b) mode to LP11b (LP11a) mode can be achieved with high conversion efficiency (more than 90%) and little polarization dependence over a wide wavelength range from 1450 nm to 1650 nm. In addition, we fabricate the proposed LP11 mode rotator using silica-based PLC. It is confirmed that the fabricated mode rotator can convert LP11a mode to LP11b mode over a wide wavelength range.

Heating systems with PLC and frequency control

International Nuclear Information System (INIS)

Abdallah, Salah; Abu-Mallouh, Riyad

2008-01-01

In this work, medium capacity controlled heating system is designed and constructed. The programming method of control of heating process is achieved by means of integrated programmable logic controller (PLC) and frequency inverter (FI). The PLC main function is to determine the required temperatures levels and the related time intervals of the heating hold time in the furnace. FI is used to control the dynamic change of temperature between various operating points. The designed system shows the capability for full control of temperature from zero to maximum for any required range of time in case of increasing or decreasing the temperature. All variables of the system will be changed gradually until reaching their needed working points. An experimental study was performed to investigate the effect of tempering temperature and tempering time on hardness and fatigue resistance of 0.4% carbon steel. It was found that increasing tempering temperature above 550 deg. C or tempering time decreases the hardness of the material. It was also found that there is a maximum number of cycles to which the specimen can survive what ever the applied load was

77 FR 4648 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-535 Series Turbofan Engine

Science.gov (United States)

2012-01-31

... Airworthiness Directives; Rolls-Royce plc (RR) RB211-535 Series Turbofan Engine AGENCY: Federal Aviation... identified in this AD, contact Rolls-Royce plc, P.O. Box 31, Derby, DE24 8BJ, United Kingdom; phone: 011 44... parts are required. Based on these figures, we estimate the cost of this AD on U.S. operators to be $1...

75 FR 63727 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-524 Series Turbofan Engines

Science.gov (United States)

2010-10-18

...-0162; Directorate Identifier 2004-NE-19-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc (RR...-2251. Contact Rolls-Royce plc, P.O. Box 31, Derby, DE24 8BJ, United Kingdom; telephone: 011-44-1332... would cost $0. Based on these figures, we estimate the total cost of the AD to U.S. operators would be...

PLC based development of control, monitoring and interlock for 100 kW, 45.6 MHz ICRH system

International Nuclear Information System (INIS)

Jadav, Hiralal; Joshi, Rameshkumar; Mali, Aniruddh K.; Kadia; Bhavesh; Parmar; Maganbhai, Kiritkumar; Kulkarni, S.V.

2015-01-01

This paper presents details of PLC based system development for 100KW at the rate 45.6 MHz. Presently in ICRH RF DAC (Data acquisition and control) system existing based on real time VME and linux operating system. The ICRH system consists of 1.5 MW RF generator operating at 22- 40MHz which is used for second harmonic heating and pre-ionization experiments on SST-1 Tokamak at 1.5T and 3T magnetic field operation respectively. The task of PLC system in RF ICRH is to control, monitoring and interlocks HVDC power supply signal. Voltage and current signal of 2 kW, 20 kW, tetrode for 100 kW RF tube electrode like Filament, Control grid, Plate, Screen grid, signal monitor and voltage set raised by PLC analog IO module. Acknowledgement of the HVDC supply Filament, Control grid, Plate, Screen grid power supply is monitor and interlocks by PLC Digital IO module to interlocks stop the RF pulse and off HV power supply. The RF pulse(shot) to trigger signal generator (5mw) RF power output feed to LPA then chain of 2 KW, 20 KW, 100 KW at the rate 45.6 MHz. The programming logic controller (PLC) software is written in ladder language for AH500 Delta make using ISP Soft 2.04 and GUI is in the table form to control and monitor the parameters. Communication of PLC to PC by ethernet LAN network. (author)

Multiprog Virtual Laboratory Applied to PLC Programming Learning

Science.gov (United States)

Shyr, Wen-Jye

2010-01-01

This study develops a Multiprog virtual laboratory for a mechatronics education designed to teach how to programme a programmable logic controller (PLC). The study was carried out with 34 students in the Department of Industry Education and Technology at National Changhua University of Education in Taiwan. In total, 17 students were assigned to…

Design of EAST LHCD high power supply feedback control system based on PLC

International Nuclear Information System (INIS)

Hu Huaichuan; Shan Jiafang

2009-01-01

Design of EAST LHCD -35kV/5.6MW high power supply feedback control system based on PLC is described. Industrial computer and PLC are used to control high power supply in the system. PID arithmetic is adopted to achieve the feedback control of voltage of high power supply. Operating system is base on real-time operating system of QNX. Good controlling properties and reliable protective properties of the feedback control system are proved by the experiment results. (authors)

British Nuclear Fuels plc: report and accounts 1987-88

International Nuclear Information System (INIS)

1989-01-01

The Energy Committee has considered the report and accounts of BNFL (British Nuclear Fuels PLC) for the year 1987-88. The report looks at BNFL as a government owned PLC - its activities and financial performance. Various questions are raised about the underlying financial position justifying the optimism portrayed in the report and accounts. The impact of cost-plus contracts on UK customers is examined. The economics of THORP (Thermal Oxide Reprocessing Plant) are also examined especially as the escalation in the cost of constructing THORP means that a substantial loss will be made in the reprocessing of waste for which contracts were signed in the late 1960s or early 1970s. The main conclusions of the report are summarized. One of these is that the UK must be cautious about becoming a repository of foreign nuclear waste. Other specific recommendations are made - some about the decommissioning of BNFL plant. (UK)

NB-PLC channel modelling with cyclostationary noise addition & OFDM implementation for smart grid

Science.gov (United States)

Thomas, Togis; Gupta, K. K.

2016-03-01

Power line communication (PLC) technology can be a viable solution for the future ubiquitous networks because it provides a cheaper alternative to other wired technology currently being used for communication. In smart grid Power Line Communication (PLC) is used to support communication with low rate on low voltage (LV) distribution network. In this paper, we propose the channel modelling of narrowband (NB) PLC in the frequency range 5 KHz to 500 KHz by using ABCD parameter with cyclostationary noise addition. Behaviour of the channel was studied by the addition of 11KV/230V transformer, by varying load location and load. Bit error rate (BER) Vs signal to noise ratio SNR) was plotted for the proposed model by employing OFDM. Our simulation results based on the proposed channel model show an acceptable performance in terms of bit error rate versus signal to noise ratio, which enables communication required for smart grid applications.

The effect of a dynamic PCL brace on patellofemoral compartment pressures in PCL-and PCL/PLC-deficient knees.

Science.gov (United States)

Welch, Tyler; Keller, Thomas; Maldonado, Ruben; Metzger, Melodie; Mohr, Karen; Kvitne, Ronald

2017-12-01

The natural history of posterior cruciate ligament (PCL) deficiency includes the development of arthrosis in the patellofemoral joint (PFJ). The purpose of this biomechanical study was to evaluate the hypothesis that dynamic bracing reduces PFJ pressures in PCL- and combined PCL/posterolateral corner (PLC)-deficient knees. Controlled Laboratory Study. Eight fresh frozen cadaveric knees with intact cruciate and collateral ligaments were included. PFJ pressures and force were measured using a pressure mapping system via a lateral arthrotomy at knee flexion angles of 30°, 60°, 90°, and 120° in intact, PCL-deficient, and PCL/PLC-deficient knees under a combined quadriceps/hamstrings load of 400 N/200 N. Testing was then repeated in PCL- and PCL/PLC-deficient knees after application of a dynamic PCL brace. Application of a dynamic PCL brace led to a reduction in peak PFJ pressures in PCL-deficient knees. In addition, the brace led to a significant reduction in peak pressures in PCL/PLC-deficient knees at 60°, 90°, and 120° of flexion. Application of the dynamic brace also led to a reduction in total PFJ force across all flexion angles for both PCL- and PCL/PLC-deficient knees. Dynamic bracing reduces PFJ pressures in PCL- and combined PCL/PLC-deficient knees, particularly at high degrees of knee flexion.

77 FR 10355 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 800 Series Turbofan Engines

Science.gov (United States)

2012-02-22

... Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 800 Series Turbofan Engines AGENCY: Federal Aviation... service of certain critical engine parts based on reduced life limits. This new AD reduces the life limits... effective March 28, 2012. ADDRESSES: For service information identified in this AD, contact Rolls-Royce plc...

The Application of PLC (Powerline Communications) in Brazil in the regulatory viewpoint; A aplicacao do PLC no Brasil do ponto de vista regulatorio

Energy Technology Data Exchange (ETDEWEB)

Vieira, Daniel; Silva Filho, Armando [Agencia Nacional de Energia Eletrica (ANEEL), Brasilia, DF (Brazil). Superintendencia de Regulacao dos Servicos de Distribuicao

2010-07-01

The Powerline Communications technology - PLC allows transmission of data, voice, image and access to high speed internet by the grid, so that a power plug can also be an access point to the network communication. Considering that the electric grid reaches the vast majority of households, there is great potential in using this technology as a tool to achieve the reality of digital inclusion in the country. However, its implementation must be accompanied by effective regulation, both through policies with respect to the conditions of use of radio frequencies, as in regulating the use of distribution networks of electricity as a means of transport for communication signal. In this context, the present article deals with the fundamental rules governing the application of PLC technology from the standpoint of the distributors and consumers of electricity, with attention to quality assurance of electrical energy and facility security.

3D digital image correlation investigation of PLC effect in a new Ni-Co base superalloy

Science.gov (United States)

Gao, Y.; Fu, S. H.; Cheng, T.; Huo, X.; Zhang, Q. C.

2013-06-01

Repeated plastic instability accompanying serrated yielding in stress-strain curves and localization of deformation is observed during plastic deformation of many metallic alloys when tensile specimens are deformed under certain experimental conditions of temperature, strain rate, and pre-deformation. This phenomenon is referred to as the Portevin- Le Chatelier (PLC) effect. TMW alloy, a newly developed Ni-Co base superalloy for aircraft engine application, also exhibit PLC effect during tensile test at temperatures ranging from 300 ° to 600 °, which are also the temperature range for engine working. In this paper, a 3D digital image correlation (3D DIC) measurement system was established to observe the localization of deformation (PLC band) in a tensile test performed on TMW alloy specimen at temperature of 400 °. The 3D DIC system, with displacement measurement accuracy up to 0.01 pixels and strain measurement accuracy up to 100 μɛ, has a high performance in displacement field calculation with more than 10000 points every second on a 3.1G Hz CPU computer. The test result shows that, the PLC bands are inclined at an angle of about 60° to the tensile axis. Unlike tensile test performed on aluminums alloy, the widths of PLC bands of TMW alloy specimen, ranging from 4 mm to 4.5 mm, are much greater than the specimen thickness (0.25 mm).

Saltwell PIC Skid Programmable Logic Controller (PLC) Software Configuration Management Plan

International Nuclear Information System (INIS)

KOCH, M.R.

1999-01-01

This document provides the procedures and guidelines necessary for computer software configuration management activities during the operation and maintenance phases of the Saltwell PIC Skids as required by LMH-PRO-309/Rev. 0, Computer Software Quality Assurance, Section 2.6, Software Configuration Management. The software configuration management plan (SCMP) integrates technical and administrative controls to establish and maintain technical consistency among requirements, physical configuration, and documentation for the Saltwell PIC Skid Programmable Logic Controller (PLC) software during the Hanford application, operations and maintenance. This SCMP establishes the Saltwell PIC Skid PLC Software Baseline, status changes to that baseline, and ensures that software meets design and operational requirements and is tested in accordance with their design basis

76 FR 78805 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 800 Series Turbofan Engines

Science.gov (United States)

2011-12-20

... Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 800 Series Turbofan Engines AGENCY: Federal Aviation... all Rolls-Royce plc (RR) RB211-Trent 800 Series Turbofan Engines. This AD results from mandatory... inspection of the FOHE mounts. We did not change the AD based on this comment. Request To Add Requirement To...

76 FR 68663 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 800 Series Turbofan Engines

Science.gov (United States)

2011-11-07

...-0755; Directorate Identifier 2010-NE-12-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc (RR... Rolls-Royce plc, Corporate Communications, P.O. Box 31, Derby, England, DE248BJ; phone: 011-44-1332... date and may amend this proposed AD based on those comments. We will post all comments we receive...

78 FR 6206 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-01-30

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT..., RB211-Trent 977-84, RB211-Trent 977B-84 and RB211-Trent 980-84 turbofan engines. This AD requires on...

A combined PLC and CPU approach to multiprocessor control

International Nuclear Information System (INIS)

Harris, J.J.; Broesch, J.D.; Coon, R.M.

1995-10-01

A sophisticated multiprocessor control system has been developed for use in the E-Power Supply System Integrated Control (EPSSIC) on the DIII-D tokamak. EPSSIC provides control and interlocks for the ohmic heating coil power supply and its associated systems. Of particular interest is the architecture of this system: both a Programmable Logic Controller (PLC) and a Central Processor Unit (CPU) have been combined on a standard VME bus. The PLC and CPU input and output signals are routed through signal conditioning modules, which provide the necessary voltage and ground isolation. Additionally these modules adapt the signal levels to that of the VME I/O boards. One set of I/O signals is shared between the two processors. The resulting multiprocessor system provides a number of advantages: redundant operation for mission critical situations, flexible communications using conventional TCP/IP protocols, the simplicity of ladder logic programming for the majority of the control code, and an easily maintained and expandable non-proprietary system

75 FR 45560 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 800 Series Turbofan Engines

Science.gov (United States)

2010-08-03

...-0755; Directorate Identifier 2010-NE-12-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc (RR... based on engine thrust rating but now based on operating shaft speeds) introduced by Rolls- Royce. The...-Royce plc, P.O. Box 31, Derby, DE24 8BJ, United Kingdom: Telephone 44 (0) 1332 242424; fax 44 (0) 1332...

76 FR 72650 - Airworthiness Directives; Rolls-Royce plc (RR) RB211 Trent 800 Series Turbofan Engines

Science.gov (United States)

2011-11-25

...-0959; Directorate Identifier 2011-NE-25-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc (RR... holidays. Fax: (202) 493-2251. Contact Rolls-Royce plc, P.O. Box 31, Derby, DE24 8BJ, United Kingdom; phone... AD. We will consider all comments received by the closing date and may amend this proposed AD based...

A fast PID controller Design for Modern PLC for Process Control Application

International Nuclear Information System (INIS)

Mirza, A.; Nafis, A.; Anees, R.M.; Idris, S.

2004-01-01

PID is the most widely used control scheme in the process industry. Pill controllers are utilized for the control of such varied parameters as pressure, flow, temperature, etc. One characteristic of these parameters is that they posses slow dynamics. Most of the available digital controllers can manipulate only a single parameter- multiple controllers are required for control of more than one parameter. The Fast PID Controller for Modem PLC (Programmable Logic Controller) developed by the authors, provides control of several parameters at a time (through a single Pill control element), enhanced programmability including variable sampling period, parameter monitoring and data storage, which may be easily implemented in a PLC. (author)

Biochemical characterization of the tomato phosphatidylinositol-specific phospholipase C (PI-PLC) family and its role in plant immunity

NARCIS (Netherlands)

Abd-El-Haliem, Ahmed; Vossen, J.H.; Zeijl, van Arjan; Dezhsetan, Sara; Testerink, Christa; Seidl, M.F.; Beck, Martina; Strutt, James; Robatzek, Silke; Joosten, M.H.A.J.

2016-01-01

Plants possess effective mechanisms to quickly respond to biotic and abiotic stresses. The rapid activation of phosphatidylinositol-specific phospholipase C (PLC) enzymes occurs early after the stimulation of plant immune-receptors. Genomes of different plant species encode multiple PLC homologs

Development of Application Programming Tool for Safety Grade PLC (POSAFE-Q)

International Nuclear Information System (INIS)

Koo, Kyungmo; You, Byungyong; Kim, Tae-Wook; Cho, Sengjae; Lee, Jin S.

2006-01-01

The pSET (POSAFE-Q Software Engineering Tool) is an application programming tool of the POSAFE-Q which is a safety graded programmable logic controller (PLC) developed for the reactor protect system of the nuclear power plant. The pSET provides an integrated development environment (IDE) which includes editors, compiler, simulator, down loader, debugger, and monitor. The pSET supports the IEC61131-3 standard software model and languages such as LD (ladder diagram) and FBD (function block diagram) which are two of the most widely used PLC programming languages in industry fields. The pSET will also support SFC (sequential function chart) language. The pSET is developed as a part of a Korea Nuclear Instrumentation and Control System (KNICS) project

Developing a Novel USB-PLC Controller for a Mechatronics Cloud Laboratory

Directory of Open Access Journals (Sweden)

Wen-Jye Shyr

2013-04-01

Full Text Available This study proposes the development and implementation of a novel Universal Serial Bus (USB-Programmable Logic Controller (PLC, called a USB-PLC controller, for a mechatronics cloud laboratory. The aim of a mechatronics cloud laboratory is to provide state of the art research quality equipment to students, allowing them to conduct hands-on experiments via the Internet. One objective of the cloud laboratory is to not only provide equipment for conducting set experiments, but also to provide a means for students to access research equipment in order to conduct individual research experiments. The proposed controller for these cloud laboratory experiments has been chosen in order to expose the students to as many different engineering and technology disciplines as possible.

A Cost Effective Solution for Development Environment for Data Acquisition, Monitoring and Simulation of PLC Controlled Applications

Directory of Open Access Journals (Sweden)

O. Bjelica

2014-06-01

Full Text Available It is very important to test and monitor the operation of Programmable Logic Controller (PLC in real time (online. Nowadays, conventional, but expensive monitoring systems for PLCs, such as Supervisory Control and Data Acquisition (SCADA systems, software and hardware simulators (or debuggers, are widely used. This paper proposes a user friendly and cost-effective development environment for monitoring, data acquisition and online simulation of applications with PLC. The purpose of this solution is to simulate the process which is controlled by the PLC. The performances of the proposed development environment are presented on the examples of washing machine and dishwasher simulators.

An Integrated Software Development Framework for PLC and FPGA based Digital I and Cs

International Nuclear Information System (INIS)

Yoo, Jun Beom; Kim, Eui Sub; Lee, Dong Ah; Choi, Jong Gyun

2014-01-01

NuDE 2.0 (Nuclear Development Environment) is a model-based software development environment for safety- critical digital systems in nuclear power plants. It makes possible to develop PLC-based systems as well as FPGA-based systems simultaneously from the same requirement or design specifications. The case study showed that the NuDE 2.0 can be adopted as an effective method of bridging the gap between the existing PLC and upcoming FPGA-based developments as well as a means of gaining diversity

An Integrated Software Development Framework for PLC and FPGA based Digital I and Cs

Energy Technology Data Exchange (ETDEWEB)

Yoo, Jun Beom; Kim, Eui Sub; Lee, Dong Ah [Konkuk University, Seoul (Korea, Republic of); Choi, Jong Gyun [KAERI, Daejeon (Korea, Republic of)

2014-08-15

NuDE 2.0 (Nuclear Development Environment) is a model-based software development environment for safety- critical digital systems in nuclear power plants. It makes possible to develop PLC-based systems as well as FPGA-based systems simultaneously from the same requirement or design specifications. The case study showed that the NuDE 2.0 can be adopted as an effective method of bridging the gap between the existing PLC and upcoming FPGA-based developments as well as a means of gaining diversity.

Design and development of PLC based offline impedance matching system for ICRH experiment

International Nuclear Information System (INIS)

Joshi, Ramesh; Jadav, H.M.; Mali, Aniruddh; Kulkarni, S.V.

2015-01-01

Ion Cyclotron Resonance Heating (ICRH) transmission line has two impedance matching networks, one for offline matching which has been employed before experimental shot. Another is online impedance matching which has been employed during experimental shot. Offline matching network consists of two static stubs, coarse tuner and coarse phase shifter identical in both transmission lines. There are motorized arrangement installed in each stubs and phase shifters. Both stubs are being used to vary transmission line length. Phase shifter is used to match the frequency of generated RF power. Programmable Logic Controller (PLC) based automation and control technique has been designed and developed for the system. Offline matching should be operated below 1 kHz frequency in order to move stepper motors. Program generates required square pulses which employed to motor controller to move either in upward or downward direction. In existing system this operation has been carried out using VME. To reduce the load on VME, PLC based system has been designed and integrated with main DAC system. WinCC software has been used (as SCADA/HMI) to develop front end GUI which communicates with OPC server. Further, OPC communicates with PLC for control of motorized arrangement. This paper describes technical details,design and development of PLC based offline matching system using WinCC as user interface. The communication between WinCC application and hardware devices was realized by OPC technique. The developed system has friendly graphical user interface, high-level automation and comprehensive function such as experimental process control. The system was proved to be reliable and accurate in practical application. (author)

Experimental Study of 6LoPLC for Home Energy Management Systems

Directory of Open Access Journals (Sweden)

Augustine Ikpehai

2016-12-01

Full Text Available Ubiquitous connectivity is already transforming residential dwellings into smart homes. As citizens continue to embrace the smart home paradigm, a new generation of low-rate and low-power communication systems is required to leverage the mass market presented by energy management in homes. Although Power Line Communication (PLC technology has evolved in the last decade, the adaptation of PLC for constrained networks is not fully charted. By adapting some features of IEEE 802.15.4 and IPv6 over Low-power Wireless Personal Area Network (6LoWPAN into power lines, this paper demonstrates a low-rate, low-power PLC system over the IPv6 network (referred to as 6LoPLC, for Home Energy Management System (HEMS applications. The overall idea is to provide a framework for assessing various scenarios that cannot be easily investigated with the limited number of evaluation hardware available. In this respect, a network model is developed in NS-3 (Version 21 to measure several important characteristics of the designed system and then validated with experimental results obtained using the Hanadu evaluation kits. Following the good agreement between the two, the NS-3 model is utilised to investigate more complex scenarios and various use-cases, such as the effects of impulsive noise, the number of nodes and packet size on the latency and Bit Error Rate (BER performances. We further demonstrate that for different network and application configurations, optimal data sizes exist. For instance, the results reveal that in order to guarantee 99% system reliability, the HEMS application data must not exceed 64 bytes. Finally, it is shown that with impulsive noise in a HEMS network comprising 50 appliances, provided the size of the payload does not exceed 64 bytes, monitoring and control applications incur a maximum latency of 238.117 ms and 248.959 ms, respectively; both of which are within acceptable limits.

Prospective radiological dose assessment. Amersham plc (Amersham site) variation application December 1998

International Nuclear Information System (INIS)

Allott, R.

2001-01-01

Amersham plc (previously Nycomed-Amersham plc) submitted an application to the Environment Agency in December 1998 for a variation to their radioactive waste discharge authorisations granted under the Radioactive Substances Act 1993. The application requested a reduction in the discharge limits for certain radionuclides and no change for the remaining radionuclides. Amersham plc undertook a further review of their discharge requirements and submitted a new assessment for revised limits in January 2001. This report provides an assessment of the radiological implications of discharges at these revised limits requested by Amersham plc and the limits proposed by the Agency. It has been prepared by the National Compliance Assessment Service at the request of Thames Region to support their determination of the application. Four candidate critical groups were identified who could be exposed to discharges from the Amersham site: 1) Sewage workers at the Maple Lodge sewage works who might be exposed to external radiation from discharges contained within sewage and inadvertently inhale or ingest sewage. 2) Anglers on the Grand Union Canal who eat a small proportion of their annual catch of freshwater fish, who drink water abstracted solely from the River Colne and eat vegetables irrigated by water from the canal. 3) Persons living closest to site who eat locally produced food. 4) Dog walkers living near to site who eat locally produced food. For continuous discharges at the Agency's proposed annual limits, the highest dose of 160 μSv/y is predicted to be received by infants who live closest to the site and eat locally produced food. Therefore, this has been identified as the critical group. Children and adults living at the same location and eating locally produced food receive doses of 140 μSv/y and 130 μSv/y respectively. The critical group dose is less than the source constraint of 300 μSv/y. The dose is dominated by direct radiation from the site (110 μSv/y) and the

77 FR 21588 - Hart and Cooley, Inc., A Subsidiary of Tomkins, PLC Including On-Site Leased Workers from...

Science.gov (United States)

2012-04-10

... Subsidiary of Tomkins, PLC Including On- Site Leased Workers from Reliable, Masiello Employment Services..., applicable to workers of Hart and Cooley, Inc., a subsidiary of Tomkins, PLC, including on-site leased... workers. Based on these findings, the Department is amending this certification to include workers leased...

75 FR 34172 - Rexam Closure Systems, Inc., a Subsidiary of Rexam PLC, Including On-Site Leased Workers From...

Science.gov (United States)

2010-06-16

... Illinois Manufacturing, Hamlet, NC; Amended Certification Regarding Eligibility To Apply for Worker... PLC, Hamlet, North Carolina. The notice was published in the Federal Register on April 23, 2010 (75 FR... at the Hamlet, North Carolina location of Rexam Closure Systems, Inc., a subsidiary of Rexam PLC. The...

Knowledge-based engineering of a PLC controlled telescope

Science.gov (United States)

Pessemier, Wim; Raskin, Gert; Saey, Philippe; Van Winckel, Hans; Deconinck, Geert

2016-08-01

As the new control system of the Mercator Telescope is being finalized, we can review some technologies and design methodologies that are advantageous, despite their relative uncommonness in astronomical instrumentation. Particular for the Mercator Telescope is that it is controlled by a single high-end soft-PLC (Programmable Logic Controller). Using off-the-shelf components only, our distributed embedded system controls all subsystems of the telescope such as the pneumatic primary mirror support, the hydrostatic bearing, the telescope axes, the dome, the safety system, and so on. We show how real-time application logic can be written conveniently in typical PLC languages (IEC 61131-3) and in C++ (to implement the pointing kernel) using the commercial TwinCAT 3 programming environment. This software processes the inputs and outputs of the distributed system in real-time via an observatory-wide EtherCAT network, which is synchronized with high precision to an IEEE 1588 (PTP, Precision Time Protocol) time reference clock. Taking full advantage of the ability of soft-PLCs to run both real-time and non real-time software, the same device also hosts the most important user interfaces (HMIs or Human Machine Interfaces) and communication servers (OPC UA for process data, FTP for XML configuration data, and VNC for remote control). To manage the complexity of the system and to streamline the development process, we show how most of the software, electronics and systems engineering aspects of the control system have been modeled as a set of scripts written in a Domain Specific Language (DSL). When executed, these scripts populate a Knowledge Base (KB) which can be queried to retrieve specific information. By feeding the results of those queries to a template system, we were able to generate very detailed "browsable" web-based documentation about the system, but also PLC software code, Python client code, model verification reports, etc. The aim of this paper is to

Design and Implementation of an over Current Protection Laboratory for Electrical Power Transmission Systems Based on PLC Techniques

Directory of Open Access Journals (Sweden)

Hassaan Th. H. Thabet

2018-01-01

Full Text Available This paper describes a modern approach for the protection of transmission lines to ensure theirsafety against the faults occurred in power systems. Our approach uses a Programmable LogicController (PLC to realize a transmission line as an over current protection relay. A conditioningcircuit was designed, implemented and tested to collect data obtained from Hall Effect sensors which convert them to suitable analog values compatible with PLC's inputs. Results obtained by our PLC control system are very similar to those obtained by the conventional relays but more efficient. An Automatic Reclosing System (ARS for remote faults is also included in this approach. Our PLC control system and its algorithm are illustrated in this paper also. This approach is designed to be used in electrical networks laboratories as an educational unit in electrical departments of engineering collages and technical institutes; it can be used also in real power systems through suitable interfacing facilities.

Slovak Electric, Plc., 1997

International Nuclear Information System (INIS)

1997-06-01

Slovenske elektrarne, a.s. (Slovak Electric, Plc.) was established on November 1, 1994 as one entity among new entities created as successors to the former Slovensky energeticky podnik. The subject activity is the generation of electric power, operation of transmission 220 kV and 400 kV systems, transit, import, export, and sales of electric power. Besides these activities the company deals with generation, distribution, and sales of heat. The company operates one nuclear power station, three thermal power plants, and thirty hydro power plants. One nuclear Power plant is under construction (state up tu June 1997). On this CD ROM next chapters are presented: (1) The structure of the company; (2) The production Units; (3) The economic power of the company; (4) The operation culture of the company; (5) The strategic plans of the company

Paths of discovery: Comparing the search effectiveness of EBSCO Discovery Service, Summon, Google Scholar, and conventional library resources.

Directory of Open Access Journals (Sweden)

Müge Akbulut

2015-09-01

Full Text Available It is becoming hard for users to select significant sources among many others as number of scientific publications increase (Henning and Gunn, 2012. Search engines that are using cloud computing methods such as Google can list related documents successfully answering user requirements (Johnson, Levine and Smith, 2009. In order to meet users’ increasing demands, libraries started to use systems which enable users to access printed and electronic sources through a single interface. This study uses quantitative and qualitative methods to compare search effectiveness between Serial Solutions Summon, EBSCO Discovery Service (EDS web discovery tools, Google Scholar (GS and conventional library databases among users from Bucknell University and Illinois Wesleyan University.

Operating experiences with programmable logic controller (PLC) system of Indian Pressurised Heavy Water Reactors (PHWR)

International Nuclear Information System (INIS)

Ughade, A.V.; Singh, Ranjeet; Bhattacharya, P.K.; Kulkarni, R.K.; Chandra, Umesh

2005-01-01

PLC system was introduced for the first time in Kaiga-1,2 and RAPS-3,4 Nuclear Power Plants (NPPs) for Station Logic Control of Non Safety Related (NSR) and Safety related (SR) systems. However, the safety system logics are still relay based. The experience on the deployment of PLC system, which is computer-based, has brought out various implementation issues. This paper give details of such experiences, the solutions emerged and applied for plants under operation/construction. (author)

Design and Implementation of PLC-Based Automatic Sun tracking System for Parabolic Trough Solar Concentrator

Directory of Open Access Journals (Sweden)

Wang Jinping

2016-01-01

Full Text Available A sun-tracking system for parabolic trough solar concentrators (PTCs is a control system used to orient the concentrator toward the sun always, so that the maximum energy can be collected. The work presented here is a design and development of PLC based sun tracking control system for PTC. Sun tracking control system consists of a Programmable Logic Controller (PLC and a single axis hydraulic drives tracking control system. Hydraulic drives and the necessary tracking angle algorithm have been designed and developed to perform the technical tasks. A PLC unit was employed to control and monitor the mechanical movement of the PTC and to collect and store data related to the tracking angle of PTC. It is found that the tracking error of the system is less than 0.6°. Field experience shows that tracking algorithm act stable and reliable and suit for PTCs.

PLC Based Automatic Multistoried Car Parking System

OpenAIRE

Swanand S .Vaze; Rohan S. Mithari

2014-01-01

This project work presents the study and design of PLC based Automatic Multistoried Car Parking System. Multistoried car parking is an arrangement which is used to park a large number of vehicles in least possible place. For making this arrangement in a real plan very high technological instruments are required. In this project a prototype of such a model is made. This prototype model is made for accommodating twelve cars at a time. Availability of the space for parking is detecte...

Effects of 5-fluorouracil on biological characteristics and drug resistance mechanisms of liver cancer cell line PLC/RAF/5

Directory of Open Access Journals (Sweden)

CHENG Kangwen

2015-09-01

Full Text Available ObjectiveTo study the changes in biological characteristics of a liver cancer cell line PLC/RAF/5 after repeated exposure to a chemotherapy drug, 5-fluorouraci (5-FU, and to investigate the relationship between drug-resistant liver cancer cells and liver cancer stem cells. MethodsA low concentration of 5-FU (1 μg/ml was used to treat the human liver cancer cell line PLC/RAF/5 repeatedly to establish the PLC/RAF/5/5-FU cell line. Morphological differences between the two types of cells were observed. The inhibitory effects of different concentrations of 5-FU (0, 0.25, 0.5, 1, 1.5, and 2 μg/ml on the proliferation of the two types of cells were determined using the CCK-8 assay. Apoptosis of the two types of cells after exposure to different concentrations of 5-FU (0.5, 1, and 2 μg/ml for 48 h was analyzed using flow cytometry. The proportions of side population cells in both types of cells were measured using flow cytometry. The colony-forming ability was compared between the two types of cells by the plate colony-forming assay. The expression of Bax, Bcl-2, ABCG2, and FoxM1 proteins in both types of cells was examined by Western blot. Between-group comparison was performed by t test. ResultsThe PLC/RAF/5/5-FU cell line was successfully established using the chemotherapy drug 5-FU. Compared with the PLC/RAF/5 cells, the PLC/RAF/5/5-FU cells had a larger volume, fewer protrusions, a changed shape of a long shuttle, and enhanced refractivity. Moreover, compared with the parent cells, the PLC/RAF/5/5-FU cells had a significantly lower sensitivity to the inhibitory effect of 5-FU on proliferation, a significantly lower proportion of cells at the G0/G1 phase of the cell cycle, significantly higher proportions of cells at the S and G2/M phases, significantly higher resistance to apoptosis, a significantly higher proportion of side population cells, and significantly enhanced proliferation (P＜0.05. According to the results of Western blot assay, the

75 FR 17630 - Airworthiness Directives; Rolls-Royce plc RB211 Trent 700 and Trent 800 Series Turbofan Engines

Science.gov (United States)

2010-04-07

...-0364; Directorate Identifier 2009-NE-27-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc... based on those comments. We will post all comments we receive, without change, to http://www.regulations... Service Information Rolls-Royce plc has issued Alert Service Bulletin RB.211-72-AG086, dated December 4...

Modeling of Hydration, Compressive Strength, and Carbonation of Portland-Limestone Cement (PLC Concrete

Directory of Open Access Journals (Sweden)

Xiao-Yong Wang

2017-01-01

Full Text Available Limestone is widely used in the construction industry to produce Portland limestone cement (PLC concrete. Systematic evaluations of hydration kinetics, compressive strength development, and carbonation resistance are crucial for the rational use of limestone. This study presents a hydration-based model for evaluating the influences of limestone on the strength and carbonation of concrete. First, the hydration model analyzes the dilution effect and the nucleation effect of limestone during the hydration of cement. The degree of cement hydration is calculated by considering concrete mixing proportions, binder properties, and curing conditions. Second, by using the gel–space ratio, the compressive strength of PLC concrete is evaluated. The interactions among water-to-binder ratio, limestone replacement ratio, and strength development are highlighted. Third, the carbonate material contents and porosity are calculated from the hydration model and are used as input parameters for the carbonation model. By considering concrete microstructures and environmental conditions, the carbon dioxide diffusivity and carbonation depth of PLC concrete are evaluated. The proposed model has been determined to be valid for concrete with various water-to-binder ratios, limestone contents, and curing periods.

78 FR 5126 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-01-24

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... turbofan engines. This AD requires replacement of the fuel oil heat exchanger (FOHE). This AD was prompted...-84 turbofan engines with a fuel oil heat exchanger (FOHE), part number 47111-1241, installed. (d...

Comportamiento de la Tecnología PLC en la Red Eléctrica

OpenAIRE

García Guibout, J.; García Garino, Carlos; Fusario, Rubén J.; Castro Lechtaler, Antonio; Sevilla, Guillermo

2007-01-01

La tecnología PLC1 - PowerLine Communications- está referida a la transmisión de datos utilizando la red eléctrica, tanto domiciliaria, como la red de distribución de baja tensión. Dependiendo del tipo de red que se utilice como soporte esta tecnología se divide en PLC indoor y outdoor. La primera se refiere a la utilización de la red domiciliaria y utiliza las frecuencias más altas de 5 MHz a 30 ó 40 MHz. La segunda, outdoor, usa la red de distribución y las frecuencias bajas de 1 MHz a 5 ó ...

PLC based automatic control of pasteurize mix in ice cream production

Science.gov (United States)

Yao, Xudong; Liang, Kai

2013-03-01

This paper describes the automatic control device of pasteurized mix in the ice cream production process.We design a scheme of control system using FBD program language and develop the programmer in the STEP 7-Micro/WIN software, check for any bugs before downloading into PLC .These developed devices will able to provide flexibility and accuracy to control the step of pasteurized mix. The operator just Input the duration and temperature of pasteurized mix through control panel. All the steps will finish automatically without any intervention in a preprogrammed sequence stored in programmable logic controller (PLC). With the help of this equipment we not only can control the quality of ice cream for various conditions, but also can simplify the production process. This control system is inexpensive and can be widely used in ice cream production industry.

78 FR 70487 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-11-26

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... turbofan engines. This AD requires removal of certain high-pressure (HP) and intermediate-pressure (IP..., RB211 Trent 768-60, 772-60, and 772B-60 turbofan engines with turbine disc part numbers (P/Ns) and...

78 FR 68360 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-11-14

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... turbofan engines. The AD number is incorrect in the Regulatory text. This document corrects that error. In... turbofan engines. As published, the AD number 2013-19-17 under Sec. 39.13 [Amended], is incorrect. No other...

78 FR 61171 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-10-03

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... (RR) RB211-535E4-B-37 series turbofan engines. This AD requires removal of affected parts using a...-B-37 series turbofan engines. (d) Unsafe Condition This AD was prompted by recalculating the lives...

Methods of Certification tests PLC-Networks in Compliance Safety Information

Directory of Open Access Journals (Sweden)

A. A. Balaev

2011-12-01

Full Text Available The aim of this research was description of the methodology of the audit plc-network to meet the requirements of information security. The technique is based on the provisions of the guidance documents and model FSTEC Russia test object methods of information on safety information.

Dynamics of plc gene transcription and α-toxin production during growth of Clostridium perfringens strains with contrasting α-toxin production

DEFF Research Database (Denmark)

Abildgaard, Lone; Schramm, Andreas; Rudi, Knut

2009-01-01

The aim of the present study was to investigate transcription dynamics of the α-toxin-encoding plc gene relative to two housekeeping genes (gyrA and rplL) in batch cultures of three Clostridium perfringens strains with low, intermediate, and high levels of α-toxin production, respectively. The plc...... transcript level was always low in the low α-toxin producing strain. For the two other strains, plc transcription showed an inducible pattern and reached a maximum level in the late exponential growth phase. The transcription levels were however inversely correlated to α-toxin production for the two strains....... We propose that this discrepancy is due to differences in plc translation rates between the strains and that strain-specific translational rates therefore must be determined before α-toxin production can be extrapolated from transcript levels in C. perfringens....

Drosophila fatty acid taste signals through the PLC pathway in sugar-sensing neurons.

Directory of Open Access Journals (Sweden)

Pavel Masek

Full Text Available Taste is the primary sensory system for detecting food quality and palatability. Drosophila detects five distinct taste modalities that include sweet, bitter, salt, water, and the taste of carbonation. Of these, sweet-sensing neurons appear to have utility for the detection of nutritionally rich food while bitter-sensing neurons signal toxicity and confer repulsion. Growing evidence in mammals suggests that taste for fatty acids (FAs signals the presence of dietary lipids and promotes feeding. While flies appear to be attracted to fatty acids, the neural basis for fatty acid detection and attraction are unclear. Here, we demonstrate that a range of FAs are detected by the fly gustatory system and elicit a robust feeding response. Flies lacking olfactory organs respond robustly to FAs, confirming that FA attraction is mediated through the gustatory system. Furthermore, flies detect FAs independent of pH, suggesting the molecular basis for FA taste is not due to acidity. We show that low and medium concentrations of FAs serve as an appetitive signal and they are detected exclusively through the same subset of neurons that sense appetitive sweet substances, including most sugars. In mammals, taste perception of sweet and bitter substances is dependent on phospholipase C (PLC signaling in specialized taste buds. We find that flies mutant for norpA, a Drosophila ortholog of PLC, fail to respond to FAs. Intriguingly, norpA mutants respond normally to other tastants, including sucrose and yeast. The defect of norpA mutants can be rescued by selectively restoring norpA expression in sweet-sensing neurons, corroborating that FAs signal through sweet-sensing neurons, and suggesting PLC signaling in the gustatory system is specifically involved in FA taste. Taken together, these findings reveal that PLC function in Drosophila sweet-sensing neurons is a conserved molecular signaling pathway that confers attraction to fatty acids.

Saltwell Leak Detector Station Programmable Logic Controller (PLC) Software Configuration Management Plan (SCMP)

International Nuclear Information System (INIS)

WHITE, K.A.

2000-01-01

This document provides the procedures and guidelines necessary for computer software configuration management activities during the operation and maintenance phases of the Saltwell Leak Detector Stations as required by HNF-PRO-309/Rev.1, Computer Software Quality Assurance, Section 2.4, Software Configuration Management. The software configuration management plan (SCMP) integrates technical and administrative controls to establish and maintain technical consistency among requirements, physical configuration, and documentation for the Saltwell Leak Detector Station Programmable Logic Controller (PLC) software during the Hanford application, operations and maintenance. This SCMP establishes the Saltwell Leak Detector Station PLC Software Baseline, status changes to that baseline, and ensures that software meets design and operational requirements and is tested in accordance with their design basis

"Kuugeln" [sprich: "Kuhg(E)ln] statt "Googeln" [sprich "Guhg(E)ln"] - Integrierte Suche mit Ebsco Discovery Service im Medienbestand der Universitätsbibliothek der Kunstuniversität Graz

OpenAIRE

Schiller, Robert

2011-01-01

"Kuugeln" rather than "Googeln". The library of the Kunstuniversität Graz has implemented EBSCO Discovery Service (EDS) as the main discovery service for searching the library's online holdings. We regard EDS as a way to offer better access to the electronic resources by using a single search technology.

Variance in total levels of phospholipase C zeta (PLC-ζ) in human sperm may limit the applicability of quantitative immunofluorescent analysis as a diagnostic indicator of oocyte activation capability.

Science.gov (United States)

Kashir, Junaid; Jones, Celine; Mounce, Ginny; Ramadan, Walaa M; Lemmon, Bernadette; Heindryckx, Bjorn; de Sutter, Petra; Parrington, John; Turner, Karen; Child, Tim; McVeigh, Enda; Coward, Kevin

2013-01-01

To examine whether similar levels of phospholipase C zeta (PLC-ζ) protein are present in sperm from men whose ejaculates resulted in normal oocyte activation, and to examine whether a predominant pattern of PLC-ζ localization is linked to normal oocyte activation ability. Laboratory study. University laboratory. Control subjects (men with proven oocyte activation capacity; n = 16) and men whose sperm resulted in recurrent intracytoplasmic sperm injection failure (oocyte activation deficient [OAD]; n = 5). Quantitative immunofluorescent analysis of PLC-ζ protein in human sperm. Total levels of PLC-ζ fluorescence, proportions of sperm exhibiting PLC-ζ immunoreactivity, and proportions of PLC-ζ localization patterns in sperm from control and OAD men. Sperm from control subjects presented a significantly higher proportion of sperm exhibiting PLC-ζ immunofluorescence compared with infertile men diagnosed with OAD (82.6% and 27.4%, respectively). Total levels of PLC-ζ in sperm from individual control and OAD patients exhibited significant variance, with sperm from 10 out of 16 (62.5%) exhibiting levels similar to OAD samples. Predominant PLC-ζ localization patterns varied between control and OAD samples with no predictable or consistent pattern. The results indicate that sperm from control men exhibited significant variance in total levels of PLC-ζ protein, as well as significant variance in the predominant localization pattern. Such variance may hinder the diagnostic application of quantitative PLC-ζ immunofluorescent analysis. Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

78 FR 20509 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-04-05

... Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking...) RB211-535E4-B-37 series turbofan engines. This proposed AD was prompted by recalculating the life of.... (c) Applicability This AD applies to Rolls-Royce plc (RR) RB211-535E4-B-37 series turbofan engines...

77 FR 39157 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2012-07-02

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT...-84, and 980-84 turbofan engines. That AD currently requires inspecting the intermediate-pressure (IP... RR model RB211-Trent 970-84, 970B-84, 972-84, 972B-84, 977-84, 977B-84, and 980-84 turbofan engines...

Performance Evaluation of CE-OFDM in PLC Channel

OpenAIRE

El ghzaoui Mohammed, Belkadid Jamal, Benbassou Ali

2011-01-01

One major drawback associated with an OFDM system is that the transmitter’soutput signal may have a high peak-to-average ratio (PAPR). High levels of PARmay be a limiting factor for power line communication (PLC) where regulatorybodies have fixed the maximum amount of transmit power. To overcome thisproblem, many approaches have been presented in the literature. One potentialsolution for reducing the peak-to-average power ratio (PAPR) in an OFDMsystem is to utilize a constant envelope OFDM (C...

75 FR 61363 - Airworthiness Directives; Rolls-Royce plc RB211-524 Series, -535 Series, RB211 Trent 700 Series...

Science.gov (United States)

2010-10-05

...-0993; Directorate Identifier 2010-NE-08-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc... Federal holidays. Fax: (202) 493-2251. Contact Rolls-Royce plc, P.O. Box 31, Derby, DE24 8BJ, United... based on those comments. We will post all comments we receive, without change, to http://www.regulations...

76 FR 29814 - Statutory Debarment and Reinstatement of BAE Systems plc

Science.gov (United States)

2011-05-23

... Bureau of Political-Military Affairs; Statutory Debarment and Reinstatement of BAE Systems plc and Policy... of Defense Trade Controls Compliance, Bureau of Political-Military Affairs, Department of State (202... required. Statutory debarment is based solely upon conviction in a criminal proceeding, conducted by a...

78 FR 70489 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-11-26

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT..., and 560A2-61 turbofan engines. This AD requires replacement or repair of the low-pressure (LP...-61, 556B2-61, 560-61, and 560A2-61 turbofan engines. (d) Reason This AD was prompted by reports of...

77 FR 58762 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2012-09-24

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT...-Trent 892-17, RB211- Trent 892B-17, and RB211-Trent 895-17 turbofan engines. That AD currently requires...-17, RB211-Trent 892B-17, and RB211-Trent 895-17 turbofan engines. (d) Unsafe Condition This AD was...

77 FR 37740 - Stagecoach Group plc and Coach USA, Inc., et al.-Acquisition of Control of Assets-American Coach...

Science.gov (United States)

2012-06-22

... Application. SUMMARY: On May 25, 2012, Stagecoach Group plc (Stagecoach), a noncarrier, and a number of its... Group plc and Coach USA, Inc., et al.--Acquisition of Control of Assets--American Coach Lines of Atlanta...) filed an application under 49 U.S.C. 14303 to acquire control of the assets of ten separate interstate...

On an LAS-integrated soft PLC system based on WorldFIP fieldbus.

Science.gov (United States)

Liang, Geng; Li, Zhijun; Li, Wen; Bai, Yan

2012-01-01

Communication efficiency is lowered and real-time performance is not good enough in discrete control based on traditional WorldFIP field intelligent nodes in case that the scale of control in field is large. A soft PLC system based on WorldFIP fieldbus was designed and implemented. Link Activity Scheduler (LAS) was integrated into the system and field intelligent I/O modules acted as networked basic nodes. Discrete control logic was implemented with the LAS-integrated soft PLC system. The proposed system was composed of configuration and supervisory sub-systems and running sub-systems. The configuration and supervisory sub-system was implemented with a personal computer or an industrial personal computer; running subsystems were designed and implemented based on embedded hardware and software systems. Communication and schedule in the running subsystem was implemented with an embedded sub-module; discrete control and system self-diagnosis were implemented with another embedded sub-module. Structure of the proposed system was presented. Methodology for the design of the sub-systems was expounded. Experiments were carried out to evaluate the performance of the proposed system both in discrete and process control by investigating the effect of network data transmission delay induced by the soft PLC in WorldFIP network and CPU workload on resulting control performances. The experimental observations indicated that the proposed system is practically applicable. Copyright © 2011 ISA. Published by Elsevier Ltd. All rights reserved.

78 FR 5 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-01-02

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT...-535E4-C-37 turbofan engines. This AD was prompted by an investigation by RR concluding that certain...- 535E4-B-37; RB211-535E4-B-75; and RB211-535E4-C-37 turbofan engines with intermediate-pressure (IP...

78 FR 37703 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-06-24

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... (RR) model RB211 Trent 768-60, 772-60, and 772B-60 turbofan engines. This AD was prompted by low...) model RB211 Trent 768-60, 772-60, and 772B-60 turbofan engines. (d) Reason This AD was prompted by low...

78 FR 22180 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-04-15

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... 972B-84, RB211-Trent 977-84, RB211-Trent 977B-84, and RB211-Trent 980-84 turbofan engines. This AD... 977B-84, and RB211-Trent 980-84 turbofan engines that incorporate RR production Modification 72-G585 or...

77 FR 20508 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2012-04-05

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT...-Trent 884B-17, RB211-Trent 892-17, RB211- Trent 892B-17, and RB211-Trent 895-17 turbofan engines. That...-17, RB211- Trent 892B-17, and RB211-Trent 895-17 turbofan engines. On September 9, 2011, we also...

What is the method in applying formal methods to PLC applications?

NARCIS (Netherlands)

Mader, Angelika H.; Engel, S.; Wupper, Hanno; Kowalewski, S.; Zaytoon, J.

2000-01-01

The question we investigate is how to obtain PLC applications with confidence in their proper functioning. Especially, we are interested in the contribution that formal methods can provide for their development. Our maxim is that the place of a particular formal method in the total picture of system

Development of Remote Monitoring and a Control System Based on PLC and WebAccess for Learning Mechatronics

OpenAIRE

Wen-Jye Shyr; Te-Jen Su; Chia-Ming Lin

2013-01-01

This study develops a novel method for learning mechatronics using remote monitoring and control, based on a programmable logic controller (PLC) and WebAccess. A mechatronics module, a Web‐CAM and a PLC were integrated with WebAccess software to organize a remote laboratory. The proposed system enables users to access the Internet for remote monitoring and control of the mechatronics module via a web browser, thereby enhancing work flexibility by enabling personnel to control mechatronics equ...

Comparative diagnostic efficacy of recombinant LLO and PI-PLC-based ELISAs for detection of listeriosis in animals.

Science.gov (United States)

Suryawanshi, Rahul D; Malik, Satya Veer Singh; Jayarao, Bhushan; Chaudhari, Sandeep P; Savage, Emily; Vergis, Jess; Kurkure, Nitin V; Barbuddhe, Sukhadeo B; Rawool, Deepak B

2017-06-01

The present study for the first time evaluates the serodiagnostic efficacy of two recombinant antigens namely, listeriolysin O (rLLO) and phosphatidyl-inositol phospholipase C (rPI-PLC). Indirect ELISA with the above recombinant antigens was used on samples collected from bovines (n=106), goats (n=138) and pigs (n=92) having either a history of abortion, emaciation and/or apparently healthy animals. Isolation of Listeria was attempted from the blood samples using USDA-FSIS method. On screening of test sera by rLLO-based ELISA, antibodies against anti-listeriolysin O (ALLO) were observed in goats (22.46%), bovines (15.10%) and pigs (16.31%). As advocated, after adsorption of positive serum samples with streptolysin O (SLO), the seropositivity for ALLO was marginally reduced (p>0.05) in goats (21.73%) and bovines (10.38%), whereas, in pigs the reduction (5.43%) was significant (pPLC-based ELISA revealed higher non-specific seropositivity for antilisterial antibodies in goats (45.65%), bovines (31.13%) and pigs (8.69%). Further, on comparing the seropositivity with isolation rate, of the 16 animals that were culturally-positive for L. monocytogenes, 15 showed ALLO positivity in unadsorbed as well as SLO-adsorbed sera by rLLO-based ELISA, however, rPI-PLC-based ELISA could detect seropositivity in only 5 animals. Moreover, rPI-PLC-based ELISA also showed seropositivity in those animals (7/30) that were culturally positive for other Listeria spp. In conclusion, rLLO can serve as a better antigen than rPI-PLC in ELISA for the serodiagnosis of listeriosis in animals; however, prior adsorption of test sera with SLO is required to avoid false positive results. Copyright © 2017 Elsevier B.V. All rights reserved.

75 FR 27964 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-22B and RB211-524 Series Turbofan Engines

Science.gov (United States)

2010-05-19

...-1157; Directorate Identifier 2009-NE-26-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc (RR... p.m., Monday through Friday, except Federal holidays. Fax: (202) 493-2251. Contact Rolls-Royce plc... received by the closing date and may amend this proposed AD based on those comments. We will post all...

Performance Evaluation of CE-OFDM in PLC Channel

OpenAIRE

El Ghzaoui Mohammed; Belkadid Jamal; Benbassou Ali

2011-01-01

One major drawback associated with an OFDM system is that the transmitter’s output signal may have a high peak-to-average ratio (PAPR). High levels of PAR may be a limiting factor for power line communication (PLC) where regulatory bodies have fixed the maximum amount of transmit power. To overcome this problem, many approaches have been presented in the literature. One potential solution for reducing the peak-to-average power ratio (PAPR) in an OFDM system is to utilize a constant envelope O...

Embedded PLC Webserver and Possibilities of its Utilization

OpenAIRE

Kopček Michal

2016-01-01

The concept Industry 4.0 is a top theme of recent times. Even if it represents the so-called 4th industrial revolution its main ideas could be used also to support the education of subjects from IT and automation. Furthermore, the basic IT and web technologies inherited by this concept are very well known to students. This paper introduces the embedded webserver of PLC and the options for its optimal utilization. The web server is essentially a simple and widely well-known technology, therefo...

78 FR 60658 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-10-02

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT..., 892-17, 892B-17, and 895-17; and RB211-524G2-T-19, -524G3-T- 19, -524H-T-36, and -524H2-T-19 turbofan... (RR) model turbofan engines that have a high-pressure (HP) compressor stage 1 to 4 rotor disc...

The application of PLC automatic control system for resin transfer in pulsed elution

International Nuclear Information System (INIS)

Long Maoxiong

2001-01-01

An application of Programmable Logic Controller (PLC) in automatic control system for resin transfer in pulsed elution column is described. The design principle as well as hardware and software are also described in detail

Development of a PLC modem for data transmission over a PWM power supply

Science.gov (United States)

Batard, Christophe; Ginot, Nicolas; Mannah, Marc Anthony; Millet, Christophe; Poitiers, Frédéric

2014-04-01

In variable-speed electrical drive and online conditioning monitoring, a feedback loop is required in order to transmit the sensor information from the motor to the controller close to the inverter. Additional cabling is used for signalling. This extra cabling has a significant cost and data transmission may not be reliable. Thus, the use of power line communication (PLC) technology to transmit data in motor drive application is quite interesting. The use of a PLC modem dedicated to the home network in a three-phase inverter-fed motor power cable does not work. Therefore, specific coupling interfaces are developed to transmit data through a pulse-width modulated power supply. Laboratory tests have shown that the couplers are operating properly. They ensure reliable data transmission in a motor drive application.

A Modification of Gamma Surveymeter Dosemeter 3007A for Monitoring Use Ethernet by PLC T100MD Series

International Nuclear Information System (INIS)

Ikhsan Shobari; Subchan, M.; Syahrudin Yusuf; Sutomo Budihardjo

2010-01-01

It has been modified a gamma surveymeter Dosemeter 3007A. The Surveymeter represents analogous surveymeter, so that an interface for data acquisition is required. Acquisition system from surveymeter is added to the voltage amplifier module from 0 - 200 mV to 0 - 5 V. This voltage value will represent of doses 0 - 5 mR/hour. Hereinafter the analogous signal 0 - 5 V as signal of input to peripheral of PLC T100MD series. Data in the form of processed analogous signal presented at local display of PLC. For long distance monitoring, data have been sent to a computer from PLC by ethernet. After this modification, the surveymeter can be used to monitor from long distance. By using Internet Service Provider, monitoring can be done at any time and any where as long as network internet is available. (author)

The Cish SH2 domain is essential for PLC-γ1 regulation in TCR stimulated CD8+ T cells.

Science.gov (United States)

Guittard, Geoffrey; Dios-Esponera, Ana; Palmer, Douglas C; Akpan, Itoro; Barr, Valarie A; Manna, Asit; Restifo, Nicholas P; Samelson, Lawrence E

2018-03-28

Cish, participates within a multi-molecular E3 ubiquitin ligase complex, which ubiquitinates target proteins. It has an inhibitory effect on T cell activation mediated by PLC-γ1 regulation, and it functions as a potent checkpoint in CD8 + T cell tumor immunotherapy. To study the structural and functional relationships between Cish and PLC-γ1 during CD8 + T cell activation, we tested mutants of the Cish-SH2 (R107K) and D/BC (L222Q, C226Q) domains. We confirmed that Cish-SH2-specific binding was essential for PLC-γ1 ubiquitination and degradation. This domain was essential for the Cish-mediated inhibition of Ca 2+ release upon TCR stimulation. No effect on inhibition of cytokine release was observed with SH2 or D/BC mutants, although the absence of Cish led to an increased release of IFN-γ and TNF-α. Using imaging we showed that Cish was expressed mostly in the cytoplasm and we did not see any Cish clustering at the plasma membrane upon stimulation. We conclude that the Cish-SH2 domain is essential for PLC-γ1 regulation in TCR-stimulated CD8 + T cells.

Development of Remote Monitoring and a Control System Based on PLC and WebAccess for Learning Mechatronics

Directory of Open Access Journals (Sweden)

Wen-Jye Shyr

2013-02-01

Full Text Available This study develops a novel method for learning mechatronics using remote monitoring and control, based on a programmable logic controller (PLC and WebAccess. A mechatronics module, a Web-CAM and a PLC were integrated with WebAccess software to organize a remote laboratory. The proposed system enables users to access the Internet for remote monitoring and control of the mechatronics module via a web browser, thereby enhancing work flexibility by enabling personnel to control mechatronics equipment from a remote location. Mechatronics control and long-distance monitoring were realized by establishing communication between the PLC and WebAccess. Analytical results indicate that the proposed system is feasible. The suitability of this system is demonstrated in the department of industrial education and technology at National Changhua University of Education, Taiwan. Preliminary evaluation of the system was encouraging and has shown that it has achieved success in helping students understand concepts and master remote monitoring and control techniques.

Perancangan Prototype Early Warning System pada Kontrol On/Off Belt Conveyor Menggunakan PLC Siemens S7-300

Directory of Open Access Journals (Sweden)

Taufik Taufik

2016-04-01

Full Text Available Nowadays, automation system become an important aspect of the manufacturing process because could make integration manufacturing process on it more effective and more efficient. PLC or Programmable Logic Controller is one kind of automation system. Many industries use PLC as automation control device in the manufacturing process to control all kind of process. For example at transportation process of coals in generator industry. Coals could be burned because main elements of coals are carbon, hydrogen, and oxygen. Base on this prototype early warning system design, we get the result if sensor thermocouple detects temperature larger than setpoint temperature (it is 2000C, then PLC will give an order to shut down the output, that is a belt conveyor. The result of this prototype design could use at coals transportation as an early warning system. The design of prototype early warning system could detect and prevent fire because of the consequence of burned coals until spreading of fire could be avoided.

One-way data transfer for PLC to VME status reporting at the Advanced Photon Source

International Nuclear Information System (INIS)

Stein, S.J.

1993-01-01

The Personnel Safety System for the experimental beamlines at the Advanced Photon Source will use a large number of Allen Bradley Programmable Logic Controllers (PLC) to replace conventional relay logic. PLCs allow for the design of a very advanced safety system that can handle a large number of I/O points. Certain situations Require monitoring of the safety system from various locations around the storage ring via EPICS OPI (operator interface)consoles. This presentation covers the method of choice for transferring data from the Personnel Safety System into an EPICS database. Specifics on PLC ladder design, EPICS database design, and hardware selection are also discussed

Embedded PLC Webserver and Possibilities of its Utilization

Science.gov (United States)

Kopček, Michal

2016-12-01

The concept Industry 4.0 is a top theme of recent times. Even if it represents the so-called 4th industrial revolution its main ideas could be used also to support the education of subjects from IT and automation. Furthermore, the basic IT and web technologies inherited by this concept are very well known to students. This paper introduces the embedded webserver of PLC and the options for its optimal utilization. The web server is essentially a simple and widely well-known technology, therefore it was chosen as an example.

Embedded PLC Webserver and Possibilities of its Utilization

Directory of Open Access Journals (Sweden)

Kopček Michal

2016-12-01

Full Text Available The concept Industry 4.0 is a top theme of recent times. Even if it represents the so-called 4th industrial revolution its main ideas could be used also to support the education of subjects from IT and automation. Furthermore, the basic IT and web technologies inherited by this concept are very well known to students. This paper introduces the embedded webserver of PLC and the options for its optimal utilization. The web server is essentially a simple and widely well-known technology, therefore it was chosen as an example.

78 FR 54152 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-09-03

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT...; -535C-37; -535E4-37; - 535E4-B-37, and -535E4-B-75 turbofan engines, and all RB211-524G2-19; - 524G3-19; -524H2-19; and -524H-36 turbofan engines. This AD requires a one-time inspection of the front combustion...

PLC-based search and secure interlock system for the personnel safety in folded tandem ion accelerator

International Nuclear Information System (INIS)

Padmakumar, Sapna; Subramanyum, N.B.V.; Bhatt, Jignesh P.; Ware, Shailaja V.; Kansara, M.J.; Gupta, S.K.; Singh, P.

2006-01-01

Safety of the personnel is one of the key issues addressed in any accelerator project. The FOTIA facility at BARC is capable of operating under standard operation conditions without any radiation hazard. Even then for a safe and reliable operation of FOTIA a PLC (Programmable logic controller) based interlock system has been implemented. This interlocking system is compact, modular, flexible, robust and easy for troubleshooting. These advantages led to the popularity of PLC rather than using a relay-based system. This paper highlights the salient features of the search and secure interlock for the personal safety of FOTIA. (author)

Process control through the incorporation of PLC networks in service radiation facilities

International Nuclear Information System (INIS)

Moehlmann, J.H.F.

1990-01-01

In order to ensure the smooth and safe running and operation of an irradiation facility, several regulations must be complied with, most of which are prescribed by the authorities. However, internal working regulations, based on years of practical experience, are of equal importance. The application of computers, micro-processors and PLCs, in combination with semi-conductor electronics, has improved process control considerably. Not only can every step in the process and even each detailed function be controlled, but also the combination of the different functions. By using PLC-systems in combination with computers, important data can be stored, recorded and presented in such a way that the technical staff can be warned in advance of breakdowns or undesirable deviations in the process parameters. Keeping a log of these data will help guarantee the correct functioning of the irradiation facility. It is even possible with these modern PLC techniques to monitor the control-console, so that each operation is recorded and accessible. (author)

Commercial information system of Slovak Electricity Transmission System, Plc

International Nuclear Information System (INIS)

Laznicka, L.

2004-01-01

Commercial Information System (CIS), which main role of is to support the commercial activities of the company, is built up by business department of SEPS, Plc in close co-operation with the supplier company Sfera, Plc. The system entries are data from electricity meters obtained by automatized data acquisition and from bugging sheets, contractual hour diagrams, parameters of transmission system like measuring schemes and formulas for calculation of demand and supply, contractual data resulting from agreements, business conditions, URSO decisions and valid legislative. A part of the system is also support of process of data evaluation like calculation of transmitted energy quantity, calculation of actual output diagrams, comparison of actual and contractual diagrams, calculation of system divergence, calculation of demand divergence, etc. The final price for the drawings of services, which are joined with transmission of the electric power will be determined by the accounting process. In this causality there are automatically made out invoices for access, losses, system charges, system services, short-term and long-term transits, divergences and for cross-border transmission auctions. System also generates various specialized synopses and statistics for the evaluation requirements of important aspects and for parameter observations. Successive liberalisation of the electricity power market, changes in legislative and URSO decisions require CIS to be sufficiently configurable and to adapt flexible on the alternating environs. (author)

Modelli di acquisto di e-book per le biblioteche dell'università italiana. Tre piattaforme a confronto (EBL, Ebrary, Ebsco

Directory of Open Access Journals (Sweden)

Giovanna Frigimelica

2014-01-01

Full Text Available In recent years, the spread of ebooks has grown exponentially. 24/7 availability, portability, the advanced tools offered by platforms make this product particularly interesting for academic libraries, which are in an experimental phase with regard to the selection and acquisition of ebooks. The article analyzes three platforms (EBL, Ebrary, EBSCO, to find out whether an Italian medium-sized university library can buy foreign titles in electronic rather than paper, and under what conditions. It provides information about titles catalog, business models, features available. The cut is practical/experimental, since the comparison among the platforms is based on a set, used as a representative sample, of 150 monographs, published in the last 5 years by different publishers and purchased on paper.

On Verification of PLC-Programs Written in the LD-Language

Directory of Open Access Journals (Sweden)

E. V. Kuzmin

2012-01-01

Full Text Available We discuss some questions connected with the construction of a technology of analysing correctness of Programmable Logic Controller programs. We consider an example of modeling and automated verification of PLC-programs written in the Ladder Diagram language (including timed function blocks of the IEC 61131-3 standard. We use the Cadence SMV for symbolic model checking. Program properties are written in the linear-time temporal logic LTL.

75 FR 33738 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-524 Series and RB211 Trent 500, 700, and 800...

Science.gov (United States)

2010-06-15

...-0562; Directorate Identifier 2009-NE-29-AD] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc (RR...-2251. Contact Rolls-Royce plc, P.O. Box 31, Derby, DE24 8BJ, United Kingdom; phone: 011 44 1332 242424... amend this proposed AD based on those comments. We will post all comments we receive, without change, to...

Cybersecurity Vulnerability Analysis of the PLC PRIME Standard

Directory of Open Access Journals (Sweden)

Miguel Seijo Simó

2017-01-01

Full Text Available Security in critical infrastructures such as the power grid is of vital importance. The Smart Grid puts power grid classical security approach on the ropes, since it introduces cyberphysical systems where devices, communications, and information systems must be protected. PoweRline Intelligent Metering Evolution (PRIME is a Narrowband Power-Line Communications (NB-PLC protocol widely used in the last mile of Advanced Metering Infrastructure (AMI deployments, playing a key role in the Smart Grid. Therefore, this work aims to unveil the cybersecurity vulnerabilities present in PRIME standard, proposing solutions and validating and discussing the results obtained.

AUTOMATION OF GLASS TEMPERING FURNACE BY USING PLC

Directory of Open Access Journals (Sweden)

Abdullah BÜYÜKYILDIZ

2007-02-01

Full Text Available In this study, a furnace which is used for observation of environments under high temperature, and also used for manufacturing of glasses which are resisted to high temperature has been designed and implemented. Automation of this system has been done by using PLC. Operating parameters of furnace such as materials entering, the furnace, the local temperature control of furnace, cooling control and materials outing have been sensed with Hall Effect Sensor. Furthermore, the observation of parameters of furnace on screen has been provided with SCADA software. Obtained products have been shown the system works successfully.

Integrated software environment dedicated for implementation of control systems based on PLC controllers

Directory of Open Access Journals (Sweden)

Szymon SURMA

2007-01-01

Full Text Available Industrial processes’ control systems based on PLC controllers play today a very important role in all fields of transport, including also sea transport. Construction of control systems is the field of engineering, which has been continuously evolving towards maximum simplification of system design path. Up to now the time needed forthe system construction from the design to commissioning had to be divided into a few stages. A mistake made in an earlier stage caused that in most cases the next stages had to be restarted. Available debugging systems allows defect detection at an early stage of theproject implementation. The paper presents general characteristic of integrated software for implementation of complex control systems. The issues related to the software use for programming of the visualisation environment, control computer, selection oftransmission medium and transmission protocol as well as PLC controllers’ configuration, software and control have been analysed.

Design and control of multifunctional sorting and training platform based on PLC control

Science.gov (United States)

Wan, Hongqiang; Ge, Shuai; Han, Peiying; Li, Fancong; Zhang, Simiao

2018-05-01

Electromechanical integration, as a multi-disciplinary subject, has been paid much attention by universities and is widely used in the automation production of enterprises. Aiming at the problem of the lack of control among enterprises and the lack of training among colleges and universities, this paper presents a design of multifunctional sorting training platform based on PLC control. Firstly, the structure of the platform is determined and three-dimensional modeling is done. Then design the platform's aerodynamic control and electrical control. Finally, realize the platform sorting function through PLC programming and configuration software development. The training platform can be used to design the practical training experiment, which has a strong advance and pertinence in the electromechanical integration teaching. At the same time, the platform makes full use of modular thinking to make the sorting modules more flexible. Compared with the traditional training platform, its teaching effect is more significant.

Automation of Underground Cable Laying Equipment Using PLC and Hmi

Science.gov (United States)

Mal Kothari, Kesar; Samba, Vishweshwar; Tania, Kinza; Udayakumar, R., Dr; Karthikeyan, Ram, Dr

2018-04-01

Underground cable laying is an alternative for overhead cable laying of telecommunication and power transmission lines. It is becoming very popular in recent times because of some of its advantages over overhead cable laying. This type of cable laying is mostly practiced in developed countries because it is more expensive than overhead cable laying. Underground cable laying is more suitable when land is not available, and it also increases the aesthetics. This paper implements the automation on a manually operated cable pulling winch machine using programmable logic controller (PLC). Winch machines are useful in underground cable laying. The main aim of the project is to replace all the mechanical functions with electrical controls which are operated through a touch screen (HMI). The idea is that the machine should shift between parallel and series circuit automatically based on the pressure sensed instead of manually operating the solenoid valve. Traditional means of throttling the engine using lever and wire is replaced with a linear actuator. Sensors such as proximity, pressure and load sensor are used to provide the input to the system. The HMI used will display the speed, length and tension of the rope being winded. Ladder logic is used to program the PLC.

Generic requirements specification for qualifying a commercially available PLC for safety-related applications in nuclear power plants. Final report

International Nuclear Information System (INIS)

Ostenso, A.; May, R.

1996-12-01

This is a specification for qualifying a commercially available PLC for application to safety systems in nuclear power plants. The specifications are suitable for evaluating a particular PLC product line as a platform for safety-related applications, establishing a suitable qualification test program, and confirming that the manufacturer has a quality assurance program that is adequate for safety-related applications or is sufficiently complete that, with a reasonable set of compensatory actions, it can be brought into conformance. The specification includes requirements for: (1) quality assurance measures applied to the qualification activities, (2) documentation to support the qualification, and (3) documentation to provide the information needed for applying the qualified PLC platform to a specific application. The specifications are designed to encompass a broad range of safety applications; however, qualifying a particular platform for a different range of applications can be accomplished by appropriate adjustments to the requirements

Fuel cycle and waste management: A perspective from British nuclear fuels plc

International Nuclear Information System (INIS)

Holmes, R.G.G.; Fairhall, G.A.; Robbins, R.A.

1996-01-01

The phrase fuel cycle and waste management implies two separate and distinct activities. British Nuclear Fuels plc (BNFL) has adopted a holistic approach to the fuel cycle that integrates the traditional fuel cycle activities of conversion to uranium hexafluoride, fuel fabrication, power generation, and reprocessing with waste arisings, its subsequent treatment, and disposal

78 FR 61168 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2013-10-03

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... (RR) RB211-535E4-37, RB211-535E4-B-37, RB211-535E4-C- 37, and RB211-535E4-B-75 turbofan engines. This...-37, RB211-535E4-B-37, RB211-535E4-C-37, and RB211-535E4-B-75 turbofan engines. (d) Unsafe Condition...

Dealing with Phrase Level Co-Articulation (PLC) in speech recognition: a first approach

NARCIS (Netherlands)

Ordelman, Roeland J.F.; van Hessen, Adrianus J.; van Leeuwen, David A.; Robinson, Tony; Renals, Steve

1999-01-01

Whereas nowadays within-word co-articulation effects are usually sufficiently dealt with in automatic speech recognition, this is not always the case with phrase level co-articulation effects (PLC). This paper describes a first approach in dealing with phrase level co-articulation by applying these

Generating Safety-Critical PLC Code From a High-Level Application Software Specification

Science.gov (United States)

2008-01-01

The benefits of automatic-application code generation are widely accepted within the software engineering community. These benefits include raised abstraction level of application programming, shorter product development time, lower maintenance costs, and increased code quality and consistency. Surprisingly, code generation concepts have not yet found wide acceptance and use in the field of programmable logic controller (PLC) software development. Software engineers at Kennedy Space Center recognized the need for PLC code generation while developing the new ground checkout and launch processing system, called the Launch Control System (LCS). Engineers developed a process and a prototype software tool that automatically translates a high-level representation or specification of application software into ladder logic that executes on a PLC. All the computer hardware in the LCS is planned to be commercial off the shelf (COTS), including industrial controllers or PLCs that are connected to the sensors and end items out in the field. Most of the software in LCS is also planned to be COTS, with only small adapter software modules that must be developed in order to interface between the various COTS software products. A domain-specific language (DSL) is a programming language designed to perform tasks and to solve problems in a particular domain, such as ground processing of launch vehicles. The LCS engineers created a DSL for developing test sequences of ground checkout and launch operations of future launch vehicle and spacecraft elements, and they are developing a tabular specification format that uses the DSL keywords and functions familiar to the ground and flight system users. The tabular specification format, or tabular spec, allows most ground and flight system users to document how the application software is intended to function and requires little or no software programming knowledge or experience. A small sample from a prototype tabular spec application is

76 FR 1985 - Airworthiness Directives; Short Brothers PLC Model SD3 Airplanes

Science.gov (United States)

2011-01-12

... Airworthiness Directives; Short Brothers PLC Model SD3 Airplanes AGENCY: Federal Aviation Administration (FAA..., at an average labor rate of $85 per work hour. Required parts cost about $10 per product. Based on.... The average labor rate is $85 per work-hour. Based on these figures, we estimate the cost of the AD on...

Model to Counter the Threats in the Data Transmission System PLC-based Technology with the Use of Petri Nets

Directory of Open Access Journals (Sweden)

A. A. Balaev

2010-06-01

Full Text Available In the article the most important aspects of information security technology Power Line Communica tions, namely the provision of conditions accessibility and integrity, are considered. It is shown that these aspects of the PLC-Technology has some internal “potential” counter, at least, such a widespread threat to both natural disturbances and/or artificial origin. The possibility of such an implementation using the simplified mathematical model of the PLC-based technologies of the theory of Petri nets is presented.

Homeplug AV and IEEE 1901 a handbook for PLC designers and users

CERN Document Server

Latchman, Haniph A; Yonge, Larry; Gavette, Sherman

2013-01-01

  The only authorized book explaining the HomePlug networking standards HomePlug is a growing technology for creating high-speed Power Line Communication (PLC) networks by transmitting data over in-home or in-office power lines. Users only need to plug adapters into wall outlets to create an instant network of computers, printers, routers, home entertainment

Histamine acting on H1 receptor promotes inhibition of proliferation via PLC, RAC, and JNK-dependent pathways

International Nuclear Information System (INIS)

Notcovich, Cintia; Diez, Federico; Tubio, Maria Rosario; Baldi, Alberto; Kazanietz, Marcelo G.; Davio, Carlos; Shayo, Carina

2010-01-01

It is well established that histamine modulates cell proliferation through the activation of the histamine H1 receptor (H1R), a G protein-coupled receptor (GPCR) that is known to couple to phospholipase C (PLC) activation via Gq. In the present study, we aimed to determine whether H1R activation modulates Rho GTPases, well-known effectors of Gq/G 11 -coupled receptors, and whether such modulation influences cell proliferation. Experiments were carried out in CHO cells stably expressing H1R (CHO-H1R). By using pull-down assays, we found that both histamine and a selective H1R agonist activated Rac and RhoA in a time- and dose-dependent manner without significant changes in the activation of Cdc42. Histamine response was abolished by the H1R antagonist mepyramine, RGS2 and the PLC inhibitor U73122, suggesting that Rac and RhoA activation is mediated by H1R via Gq coupling to PLC stimulation. Histamine caused a marked activation of serum response factor activity via the H1R, as determined with a serum-responsive element (SRE) luciferase reporter, and this response was inhibited by RhoA inactivation with C3 toxin. Histamine also caused a significant activation of JNK which was inhibited by expression of the Rac-GAP β2-chimaerin. On the other hand, H1R-induced ERK1/2 activation was inhibited by U73122 but not affected by C3 or β2-chimaerin, suggesting that ERK1/2 activation was dependent on PLC and independent of RhoA or Rac. [ 3 H]-Thymidine incorporation assays showed that both histamine and the H1R agonist inhibited cell proliferation in a dose-dependent manner and that the effect was independent of RhoA but partially dependent on JNK and Rac. Our results reveal that functional coupling of the H1R to Gq-PLC leads to the activation of RhoA and Rac small GTPases and suggest distinct roles for Rho GTPases in the control of cell proliferation by histamine.

Metodología para la implementación de controlador difuso tipo Takagi-Sugeno en PLC s7-300

Directory of Open Access Journals (Sweden)

Cristian Guarnizo Lemus

2011-12-01

Full Text Available This paper presents a methodology for implementing fuzzy logic controllers based on a S7-300 PLC using the programming language SCL(Structured Control Language in STEP 7. We present the design of the fuzzy function, variable declarations, and the evaluation design of membership functions and rules of the fuzzy system. Since this scheme can be implemented more complex fuzzy models, such as adaptive or Autotuner. We present an application example for a discrete time simulated in the PLC using a PI fuzzy controller.

Design of monitoring system for mail-sorting based on the Profibus S7 series PLC

Science.gov (United States)

Zhang, W.; Jia, S. H.; Wang, Y. H.; Liu, H.; Tang, G. C.

2017-01-01

With the rapid development of the postal express, the workload of mail sorting is increasing, but the automatic technology of mail sorting is not mature enough. In view of this, the system uses Siemens S7-300 PLC as the main station controller, PLC of Siemens S7-200/400 is from the station controller, through the man-machine interface configuration software MCGS, PROFIBUS-DP communication, RFID technology and mechanical sorting hand achieve mail classification sorting monitoring. Among them, distinguish mail-sorting by scanning RFID posted in the mail electronic bar code (fixed code), the system uses the corresponding controller on the acquisition of information processing, the processed information transmit to the sorting manipulator by PROFIBUS-DP. The system can realize accurate and efficient mail sorting, which will promote the development of mail sorting technology.

Comparative analysis of different configurations of PLC-based safety systems from reliability point of view

Science.gov (United States)

Tapia, Moiez A.

1993-01-01

The study of a comparative analysis of distinct multiplex and fault-tolerant configurations for a PLC-based safety system from a reliability point of view is presented. It considers simplex, duplex and fault-tolerant triple redundancy configurations. The standby unit in case of a duplex configuration has a failure rate which is k times the failure rate of the standby unit, the value of k varying from 0 to 1. For distinct values of MTTR and MTTF of the main unit, MTBF and availability for these configurations are calculated. The effect of duplexing only the PLC module or only the sensors and the actuators module, on the MTBF of the configuration, is also presented. The results are summarized and merits and demerits of various configurations under distinct environments are discussed.

Trust and Collaboration in PLC Teams: Teacher Relationships, Principal Support, and Collaborative Benefits

Science.gov (United States)

Hallam, Pamela R.; Smith, Henry R.; Hite, Julie M.; Hite, Steven J.; Wilcox, Bradley R.

2015-01-01

Professional learning communities (PLCs) are being recognized as effective in improving teacher collaboration and student achievement. Trust is critical in effectively implementing the PLC model, and the school principal is best positioned to influence school trust levels. Using five facets of trust, this research sought to clarify the impact of…

Sequence variation in the alpha-toxin encoding plc gene of Clostridium perfringens strains isolated from diseased and healthy chickens

DEFF Research Database (Denmark)

Abildgaard, L; Engberg, RM; Pedersen, Karl

2009-01-01

The aim of the present study was to analyse the genetic diversity of the alpha-toxin encoding plc gene and the variation in a-toxin production of Clostridium perfringens type A strains isolated from presumably healthy chickens and chickens suffering from either necrotic enteritis (NE) or cholangio......-hepatitis. The a-toxin encoding plc genes from 60 different pulsed-field gel electrophoresis (PFGE) types (strains) of C perfringens were sequenced and translated in silico to amino acid sequences and the a-toxin production was investigated in batch cultures of 45 of the strains using an enzyme...

Enhanced Operation of Electricity Distribution Grids Through Smart Metering PLC Network Monitoring, Analysis and Grid Conditioning

Directory of Open Access Journals (Sweden)

Iker Urrutia

2013-01-01

Full Text Available Low Voltage (LV electricity distribution grid operations can be improved through a combination of new smart metering systems’ capabilities based on real time Power Line Communications (PLC and LV grid topology mapping. This paper presents two novel contributions. The first one is a new methodology developed for smart metering PLC network monitoring and analysis. It can be used to obtain relevant information from the grid, thus adding value to existing smart metering deployments and facilitating utility operational activities. A second contribution describes grid conditioning used to obtain LV feeder and phase identification of all connected smart electric meters. Real time availability of such information may help utilities with grid planning, fault location and a more accurate point of supply management.

Study on the Development of Builtin PLC%内嵌式PLC的设计与实现

Institute of Scientific and Technical Information of China (English)

刘家亮; 熊晓红; 黄树槐

2001-01-01

介绍了PLC编程语言——语句表的结构和特点,讨论了语句表解释程序的词法分析和语法分析技术,以及运用面向对象的编程思想、构造语句表解释程序，实现内嵌式PLC等问题。%In this thesis, the architecture and character of the PLC languageSTL are introduced. And then, the structure of STL interpreting program and the technique of lexical analysis and syntax analysis are discussed. Develop the STL interpreting program with the idea of OOP(objectoriented programming), realized the builtin PLC are also attempted.

Power supply system on HT-7 tokamak for diagnostic neutral beam based on PLC

International Nuclear Information System (INIS)

Zhang Jian; Liu Baohua; Ding Tonghai; Du Shaowu

2006-01-01

A power supply system for diagnostic neutral beam on the HT-7 Tokamak was developed. Its logic control system based on S7-300 PLC was described. The experimental results show that the system is easy to operate and its performance is reliable. (authors)

Protease-activated receptor-2 stimulates intestinal epithelial chloride transport through activation of PLC and selective PKC isoforms.

Science.gov (United States)

van der Merwe, Jacques Q; Moreau, France; MacNaughton, Wallace K

2009-06-01

Serine proteases play important physiological roles through their activity at G protein-coupled protease-activated receptors (PARs). We examined the roles that specific phospholipase (PL) C and protein kinase (PK) C (PKC) isoforms play in the regulation of PAR(2)-stimulated chloride secretion in intestinal epithelial cells. Confluent SCBN epithelial monolayers were grown on Snapwell supports and mounted in modified Ussing chambers. Short-circuit current (I(sc)) responses to basolateral application of the selective PAR(2) activating peptide, SLIGRL-NH(2), were monitored as a measure of net electrogenic ion transport caused by PAR(2) activation. SLIGRL-NH(2) induced a transient I(sc) response that was significantly reduced by inhibitors of PLC (U73122), phosphoinositol-PLC (ET-18), phosphatidylcholine-PLC (D609), and phosphatidylinositol 3-kinase (PI3K; LY294002). Immunoblot analysis revealed the phosphorylation of both PLCbeta and PLCgamma following PAR(2) activation. Pretreatment of the cells with inhibitors of PKC (GF 109203X), PKCalpha/betaI (Gö6976), and PKCdelta (rottlerin), but not PKCzeta (selective pseudosubstrate inhibitor), also attenuated this response. Cellular fractionation and immunoblot analysis, as well as confocal immunocytochemistry, revealed increases of PKCbetaI, PKCdelta, and PKCepsilon, but not PKCalpha or PKCzeta, in membrane fractions following PAR(2) activation. Pretreatment of the cells with U73122, ET-18, or D609 inhibited PKC activation. Inhibition of PI3K activity only prevented PKCdelta translocation. Immunoblots revealed that PAR(2) activation induced phosphorylation of both cRaf and ERK1/2 via PKCdelta. Inhibition of PKCbetaI and PI3K had only a partial effect on this response. We conclude that basolateral PAR(2)-induced chloride secretion involves activation of PKCbetaI and PKCdelta via a PLC-dependent mechanism resulting in the stimulation of cRaf and ERK1/2 signaling.

The electromagnetic compatibility of the PLC technology; La compatibilite electromagnetique de la technologie CPL. Des donnees sur les lignes electriques

Energy Technology Data Exchange (ETDEWEB)

Bermudez, J.-L.; Marthe, E.; Rachidi, F.; Rubinstein, M.; Vukicevic, A.

2006-07-01

PLC (Power Line Communications) in Europe or BPL (Broadband over Power Lines) in the US are techniques that allows the utilization of low and middle voltage power lines for the transmission of high frequencies by coupling electrical signals to them. In this way, unshielded power lines may be used for the transmission of numerical information similar to the well known xDSL-techniques using telephone lines. Typically, the frequency range used by PLC is between 9 kHz and 30 MHz. The authors describe the general principles and investigate possible electromagnetic interference with existing radio frequency equipment like xDSL, CATV (Cable Television) and Wireless (GSM, Bluetooth, WiFi). Finally, they present theoretical and experimental results of techniques allowing the reduction of such interference by reducing the emission of electromagnetic signals from PLC systems. The preferred method is based on an active control by injecting an auxiliary signal into the third conductor (ground)

Turnaround and Sustainability in Economic Crisis: The Case of NEXT PLC

OpenAIRE

Zhang, Biyan

2013-01-01

Purpose- against a background of continued weak economic conditions and with many firms experiencing declining financial performance, this paper presents a synthesis of the business turnaround literature and combines with analysis case –NEXT plc. It purposes to discus the turnaround strategies can keep the declining firm sustainability recovery, during the economic cycle. \\ud \\ud Design/methodology/approach- the turnaround strategy for sustainability recovery are examined by a case study of N...

Formal verification of complex properties on PLC programs

CERN Document Server

Darvas, D; Voros, A; Bartha, T; Blanco Vinuela, E; Gonzalez Suarez, V M

2014-01-01

Formal verification has become a recommended practice in the safety-critical application areas. However, due to the complexity of practical control and safety systems, the state space explosion often prevents the use of formal analysis. In this paper we extend our former verification methodology with effective property preserving reduction techniques. For this purpose we developed general rule-based reductions and a customized version of the Cone of Influence (COI) reduction. Using these methods, the verification of complex requirements formalised with temporal logics (e.g. CTL, LTL) can be orders of magnitude faster. We use the NuSMV model checker on a real-life PLC program from CERN to demonstrate the performance of our reduction techniques.

Evaluation of a fast PLC module in prospect of the LHC beam interlock system

CERN Document Server

Zaera-Sanz, Manuel

2005-01-01

The LHC Beam Interlock system requires a controller performing a simple matrix function to collect the different beam dump requests. To satisfy the expected safety level of the Interlock, the system should be robust and reliable. The PLC is a promising candidate to fulfil both aspects but too slow to meet the expected response time which is of the order of mseconds. Siemens has introduced a “so called” fast module (FM352-5 Boolean Processor) that provides independent and extremely fast control of a process within a larger control system using an onboard processor, a Field Programmable Gate Array (FPGA), to execute code in parallel which results in extremely fast scan times. It is interesting to investigate its features and to evaluate it as a possible candidate for the beam interlock system. This note publishes the results of this study. As well, this note could be useful for other applications requiring fast processing using a PLC.

Design and application of the high-voltage DC power-supply control system based on PLC

International Nuclear Information System (INIS)

Huang Yiyun; Zheng Guanghua; Wu Junshuan; Yang Chunsheng; Hu Huaichuan

2002-03-01

The design and application of A kind of high-voltage DC power-supply control system based on PLC is referred, in addition, KingView is used to monitor the system in real time and manage the man-machine conversation ideally

Měření výpočetního výkonu PLC PFC200

OpenAIRE

Majchrák, František

2017-01-01

V práci sa analyzujú možnosti merania výpočetného výkonu PLC PFC200 od firmy WAGO. Prvú časť práce venujem priblíženiu funkčnosti automatu, jeho využitiu, jednotlivým možnostiam programovania, zoznamujem sa s implementáciou vlastných programových blokov v prostredí embedded Linux a zisťujem jeho prepojenie s PLC prostredím. V druhej časti práce sa sústredím na meranie výkonu, jeho typy, popis certifikovaných meraní a jeho celkové zhodnotenie. V poslednej časti je vysvetlená aplikácia, ktorá z...

Design of a PLC control program for a batch plant : VHS case study 1

NARCIS (Netherlands)

Mader, A.H.; Brinksma, E.; Wupper, H.; Bauer, N.

2001-01-01

This article reports on the systematic design and validation of a PLC control program for the batch plant that has been selected as a case study for the EC project on Verification of Hybrid Systems (VHS). We show how a correct design of the control program can be obtained in an incremental manner

Design of a PLC Control Program for a Batch Plant - VHS Case Study 1

NARCIS (Netherlands)

Mader, Angelika H.; Brinksma, Hendrik; Wupper, H.; Bauer, N.

2001-01-01

This article reports on the systematic design and validation of a PLC control program for the batch plant that has been selected as a case study for the EC project on Verification of Hybrid Systems (VHS). We show how a correct design of the control program can be obtained in an incremental manner

Enhanced expression of Gqα and PLC-β1 proteins contributes to vascular smooth muscle cell hypertrophy in SHR: role of endogenous angiotensin II and endothelin-1.

Science.gov (United States)

Atef, Mohammed Emehdi; Anand-Srivastava, Madhu B

2014-07-01

Vascular Gqα signaling has been shown to contribute to cardiac hypertrophy. In addition, angiotensin II (ANG II) was shown to induce vascular smooth muscle cell (VSMC) hypertrophy through Gqα signaling; however, the studies on the role of Gqα and PLC-β1 proteins in VSMC hypertrophy in animal model are lacking. The present study was therefore undertaken to examine the role of Gqα/PLC-β1 proteins and the signaling pathways in VSMC hypertrophy using spontaneously hypertensive rats (SHR). VSMC from 16-wk-old SHR and not from 12-wk-old SHR exhibited enhanced levels of Gqα/PLC-β1 proteins compared with age-matched Wistar-Kyoto (WKY) rats as determined by Western blotting. However, protein synthesis as determined by [(3)H]leucine incorporation was significantly enhanced in VSMC from both 12- and 16-wk-old SHR compared with VSMC from age-matched WKY rats. Furthermore, the knockdown of Gqα/PLC-β1 in VSMC from 16-wk-old SHR by antisense and small interfering RNA resulted in attenuation of protein synthesis. In addition, the enhanced expression of Gqα/PLC-β1 proteins, enhanced phosphorylation of ERK1/2, and enhanced protein synthesis in VSMC from SHR were attenuated by the ANG II AT1 and endothelin-1 (ET-1) ETA receptor antagonists losartan and BQ123, respectively, but not by the ETB receptor antagonist BQ788. In addition, PD98059 decreased the enhanced expression of Gqα/PLC-β1 and protein synthesis in VSMC from SHR. These results suggest that the enhanced levels of endogenous ANG II and ET-1 through the activation of AT1 and ETA receptors, respectively, and MAP kinase signaling, enhanced the expression of Gqα/PLC-β1 proteins in VSMC from 16-wk-old SHR and result in VSMC hypertrophy. Copyright © 2014 the American Physiological Society.

Amarogentin, a secoiridoid glycoside, abrogates platelet activation through PLC γ 2-PKC and MAPK pathways.

Science.gov (United States)

Yen, Ting-Lin; Lu, Wan-Jung; Lien, Li-Ming; Thomas, Philip Aloysius; Lee, Tzu-Yin; Chiu, Hou-Chang; Sheu, Joen-Rong; Lin, Kuan-Hung

2014-01-01

Amarogentin, an active principle of Gentiana lutea, possess antitumorigenic, antidiabetic, and antioxidative properties. Activation of platelets is associated with intravascular thrombosis and cardiovascular diseases. The present study examined the effects of amarogentin on platelet activation. Amarogentin treatment (15~60  μM) inhibited platelet aggregation induced by collagen, but not thrombin, arachidonic acid, and U46619. Amarogentin inhibited collagen-induced phosphorylation of phospholipase C (PLC) γ2, protein kinase C (PKC), and mitogen-activated protein kinases (MAPKs). It also inhibits in vivo thrombus formation in mice. In addition, neither the guanylate cyclase inhibitor ODQ nor the adenylate cyclase inhibitor SQ22536 affected the amarogentin-mediated inhibition of platelet aggregation, which suggests that amarogentin does not regulate the levels of cyclic AMP and cyclic GMP. In conclusion, amarogentin prevents platelet activation through the inhibition of PLC γ2-PKC cascade and MAPK pathway. Our findings suggest that amarogentin may offer therapeutic potential for preventing or treating thromboembolic disorders.

Evaluation and application of a fast module in a PLC based interlock and control system

International Nuclear Information System (INIS)

Zaera-Sanz, M

2009-01-01

The LHC Beam Interlock system requires a controller performing a simple matrix function to collect the different beam dump requests. To satisfy the expected safety level of the Interlock, the system should be robust and reliable. The PLC is a promising candidate to fulfil both aspects but too slow to meet the expected response time which is of the order of μseconds. Siemens has introduced a 'so called' fast module (FM352-5 Boolean Processor). It provides independent and extremely fast control of a process within a larger control system using an onboard processor, a Field Programmable Gate Array (FPGA), to execute code in parallel which results in extremely fast scan times. It is interesting to investigate its features and to evaluate it as a possible candidate for the beam interlock system. This paper publishes the results of this study. As well, this paper could be useful for other applications requiring fast processing using a PLC.

75 FR 27973 - Airworthiness Directives; Rolls-Royce plc RB211-524C2 Series Turbofan Engines

Science.gov (United States)

2010-05-19

... RB211-524C2 Series Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice... Rolls-Royce plc (RR) model RB211-524C2-19 and RB211-524C2-B-19 turbofan engines. These engines are...

Controlling the Filling and Capping Operation of a Bottling Plant using PLC and SCADA

Directory of Open Access Journals (Sweden)

Kunal Chakraborty

2015-03-01

Full Text Available This paper presents basic stages of operation of a bottling plant, i.e. the filling and capping process. The main aim of our paper is to control the filling and capping section of a bottling plant simultaneously. At first a set of empty bottle is run by using a conveyer towards filling section, after the operation, the filled bottles are sent towards the capping section. After successful capping operation, the sealed bottles terminate towards exit and a new set of empty bottle arrive, in this way the process continues. This paper includes the method using which, a bunch of bottles can be filled and capped at one instant of time. This method has made the operation more flexible and time saving. The filling and capping operations are controlled using Programmable Logic Controllers (PLC, as the PLC’s are very much user-efficient, cost-effective and easy to control. By using PLC automation the whole process is kept under control. SCADA (Supervisory Control and Data Acquisition is used to monitor the process by means of a display system.

78 FR 65313 - Actavis, Inc. a corporation, and Warner Chilott PLC; Analysis of Agreement Containing Consent...

Science.gov (United States)

2013-10-31

... FEDERAL TRADE COMMISSION [File No. 131 0152] Actavis, Inc. a corporation, and Warner Chilott PLC... https://ftcpublic.commentworks.com/ftc/actaviswarnerconsent following the instructions on the web-based....com/ftc/actaviswarnerconsent by following the instructions on the web-based form. If this Notice...

76 FR 25353 - Hikma Pharmaceuticals PLC; Analysis of Agreement Containing Consent Orders To Aid Public Comment

Science.gov (United States)

2011-05-04

... FEDERAL TRADE COMMISSION [File No. 111 0051] Hikma Pharmaceuticals PLC; Analysis of Agreement...-based form. To ensure that the Commission considers an electronic comment, you must file it on the Web-based form at the Web link: https://ftcpublic.commentworks.com/ftc/hikmabaxter . If this Notice appears...

PLC SCADA systém pro řízení větrného tunelu

OpenAIRE

Trenčan, Tomáš

2017-01-01

Předložená bakalářská práce se věnuje programovatelnému automatu (PLC) firmy WAGO a SCADA systémům. Skládá se ze dvou části, z teoretické části a z praktické části. V teoretické části jsou na zakladě poznatků z odborné literatury vymezeny pojmy ohledně SCADA systémů, PLC od firmy WAGO a vývojového prostředí e!COCKPIT firmy WAGO a rešerše volně dostupných SCADA systémů podporujících Modbus TCP. Na základě této rešerše byl vybrán SCADA systém pro vizualizaci dvou demonstračníc úloh v praktické ...

Crystallization, optimization and preliminary X-ray characterization of a metal-dependent PI-PLC from Streptomyces antibioticus

International Nuclear Information System (INIS)

Jackson, Michael R.; Selby, Thomas L.

2012-01-01

Crystallization and diffraction analysis of a Ca 2+ -dependent PI-PLC from Streptomyces is reported. Optimization of crystals was completed using a drop-pinning technique and heavy-atom soaks to achieve high-quality diffraction to 1.23 Å. A recombinant metal-dependent phosphatidylinositol-specific phospholipase C (PI-PLC) from Streptomyces antibioticus has been crystallized by the hanging-drop method with and without heavy metals. The native crystals belonged to the orthorhombic space group P222, with unit-cell parameters a = 41.26, b = 51.86, c = 154.78 Å. The X-ray diffraction results showed significant differences in the crystal quality of samples soaked with heavy atoms. Additionally, drop pinning, which increases the surface area of the drops, was also used to improve crystal growth and quality. The combination of heavy-metal soaks and drop pinning was found to be critical for producing high-quality crystals that diffracted to 1.23 Å resolution

Melatonin potentiates glycine currents through a PLC/PKC signalling pathway in rat retinal ganglion cells.

Science.gov (United States)

Zhao, Wen-Jie; Zhang, Min; Miao, Yanying; Yang, Xiong-Li; Wang, Zhongfeng

2010-07-15

In vertebrate retina, melatonin regulates various physiological functions. In this work we investigated the mechanisms underlying melatonin-induced potentiation of glycine currents in rat retinal ganglion cells (RGCs). Immunofluorescence double labelling showed that rat RGCs were solely immunoreactive to melatonin MT(2) receptors. Melatonin potentiated glycine currents of RGCs, which was reversed by the MT(2) receptor antagonist 4-P-PDOT. The melatonin effect was blocked by intracellular dialysis of GDP-beta-S. Either preincubation with pertussis toxin or application of the phosphatidylcholine (PC)-specific phospholipase C (PLC) inhibitor D609, but not the phosphatidylinositol (PI)-PLC inhibitor U73122, blocked the melatonin effect. The protein kinase C (PKC) activator PMA potentiated the glycine currents and in the presence of PMA melatonin failed to cause further potentiation of the currents, whereas application of the PKC inhibitor bisindolylmaleimide IV abolished the melatonin-induced potentiation. The melatonin effect persisted when [Ca(2+)](i) was chelated by BAPTA, and melatonin induced no increase in [Ca(2+)](i). Neither cAMP-PKA nor cGMP-PKG signalling pathways seemed to be involved because 8-Br-cAMP or 8-Br-cGMP failed to cause potentiation of the glycine currents and both the PKA inhibitor H-89 and the PKG inhibitor KT5823 did not block the melatonin-induced potentiation. In consequence, a distinct PC-PLC/PKC signalling pathway, following the activation of G(i/o)-coupled MT(2) receptors, is most likely responsible for the melatonin-induced potentiation of glycine currents of rat RGCs. Furthermore, in rat retinal slices melatonin potentiated light-evoked glycine receptor-mediated inhibitory postsynaptic currents in RGCs. These results suggest that melatonin, being at higher levels at night, may help animals to detect positive or negative contrast in night vision by modulating inhibitory signals largely mediated by glycinergic amacrine cells in the inner

75 FR 15321 - Airworthiness Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines

Science.gov (United States)

2010-03-29

... Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines AGENCY: Federal Aviation Administration... Based on the service information, we estimate that this AD will affect about 138 RB211 Trent 800 series... will cost about $2,000 per engine. Based on these figures, we estimate the cost of the AD on U.S...

PLC-Based Pressure Control in Multi-Pump Applications

Directory of Open Access Journals (Sweden)

Vodovozov Valery

2015-12-01

Full Text Available The paper is devoted to the centrifugal pumps represented the most popular type of pumping equipment used in different areas. The pressure control approach for variable speed driven (VSD parallel connected centrifugal pumps is reported. The goal of the study is optimization of some quality indices, such as efficiency, consumed power, productivity, energy carrier temperature, heat irradiation, etc. One of them – efficiency – has been studied in the paper more carefully. The mathematical model of pumping process is discussed and a vector-matrix description of the multi-pump application is given. The program-based pressure control system is developed which productivity is changed by regulating the number of working pumps. The paper introduces new pressure control algorithms based on the working point estimation intended for programmable logical controllers (PLC. Experiments prove correctness of the offered methodology.

Development of a measurement and control system for a 10 kW@20 K refrigerator based on Siemens PLC S7-300

Science.gov (United States)

Li, J.; Liu, L. Q.; Liu, T.; Xu, X. D.; Dong, B.; Lu, W. H.; Pan, W.; Wu, J. H.; Xiong, L. Y.

2017-02-01

A 10 kW@20 K refrigerator has been established by the Technical Institute of Physics and Chemistry, Chinese Academy of Sciences. A measurement and control system based on Siemens PLC S7-300 for this 10 kW@20 K refrigerator is developed. According to the detailed measurement requirements, proper sensors and transmitters are adopted. Siemens S7-300 PLC CPU315-2 PN/DP operates as a master station. Two sets of ET200M DP remote expand I/O, one power meter, two compressors and one vacuum gauge operate as slave stations. Profibus-DP field communication and Modbus communication are used between the master station and the slave stations in this control system. The upper computer HMI (Human Machine Interface) is compiled using Siemens configuration software WinCC V7.0. The upper computer communicates with PLC by means of industrial Ethernet. After commissioning, this refrigerator has been operating with a 10 kW of cooling power at 20 K for more than 72 hours.

Effective time management for high performance in an organization Case: Lasaco Assurance PLC

OpenAIRE

Adeojo, Adeyinka

2012-01-01

The main objective of this thesis is to determine the effect of time management on high organizational performance using LASACO ASSURANCE Plc. as a case company. In this thesis, the employees working with the company were sent questionnaires. Their responses were critically analyzed and thus related to the theories. A quantitative approach was used as the methodology. According to the theory, time management is a method for managers to increase work performance effectiveness. Time man...

Increase in Multicast OFDM Data Rate in PLC Network using Adaptive LP-OFDM

OpenAIRE

Maiga , Ali; Baudais , Jean-Yves; Hélard , Jean-François

2009-01-01

ISBN: 978-1-4244-3523-4; International audience; Linear precoding (LP) technique applied to OFDM systems has already proved its ability to significantly increase the system throughput in a powerline communication (PLC) context. In this paper, we propose resource allocation algorithms based on the LP technique to increase the multicast OFDM systems bit rate. The conventional multicast capacity is limited by the user which experiences the worst channel conditions. To increase the multicast bit ...

High-speed narrowband PLC - High-performance Access Powerline Communication structures in smart grid; High-Speed Narrowband PLC. Leistungsfaehige Access-Powerline-Kommunikationsstrukturen im Smart Grid

Energy Technology Data Exchange (ETDEWEB)

Offner, Georg [devolo AG, Aachen (Germany)

2012-07-01

The smart grid provides a stable operation of a decentralized power system which is fed more and more by small providers by means of solar technology and wind power technology. Furthermore, commercial and private clients profit from the smart grid, as they may capture information about their current consumption in real time or can obtain these data from the Internet. The author of the contribution under consideration presents new approaches to access powerline communications that enable efficient communication between IPv6 based household meter and network station. The contribution under consideration describes the technologies used as well as practical experiences and initial results from field tests. Here insights from the integration of G3-PLC are in the foreground.

Development of a Measurement and Control System for a 40l/h Helium Liquefier based on Siemens PLC S7-300

Science.gov (United States)

Li, J.; Liu, L. Q.; Xu, X. D.; Liu, T.; Li, Q.; Hu, Z. J.; Wang, B. M.; Xiong, L. Y.; Dong, B.; Yan, T.

A 40l/h Helium Liquefier has been commissioned by the Technical Institute of Physics and Chemistry, Chinese Academy of Sciences. A measurement and control system based on Siemens PLC S7-300 for this Helium Liquefier is developed. Proper sensors are selected, for example, three types of transmitters are adopted respectively according to detailed temperature measurement requirements. Siemens S7-300 PLC CPU315-2PN/DP operates as a master station and three sets of ET200 M DP remote expand I/O operate asslave stations. Profibus-DP field communication is used between the master station and the slave stations. The upper computer HMI(Human Machine Interface) is compiled using Siemens configuration software WinCC V7.0. The upper computer communicates with PLC by means of industrial Ethernet. A specific control logic for this Helium Liquefier is developed. The control of the suction and discharge pressures of the compressor and the control of the turbo-expanders loop are being discussed in this paper. Following the commissioning phase, the outlet temperature of the second stage turbine has reached 8.6K and the temperature before the throttle valve has reached 13.1K.

Construction of a multi-vender fieldbus system for PC-based control using soft PLC

Energy Technology Data Exchange (ETDEWEB)

Park, J. H.; Park, Y. J.; Choi, J. M.; Park, J. S. [Hankyong National Univ., Ansung (Korea)

2000-12-01

The relationship between the IEC 61131-3 programming language and the fieldbus application that became necessary as an outcome of the recent automation technology was described.An educational text for the field users on the integration of the Input/Output (I/O) modules and on the IEC 61131-3 programming language was prepared. An attempt was made to include description of the various kind of the fieldbuses and promote understanding of the CAN and the AS-I bus using a practical approach. The profibus is most widely used in the world, and thus is an important and a standardized tool in the area of the process control. A method of integrating the profibus with the most commonly used I/O modules in the field was suggested. The most essential characteristics of the IEC 61131-3 programming language was also described. A method for the construction of the multi-vender I/O modules by the Soft PLC 4C of the softings was suggested. Various technical steps are necessary for the integration of the I/O modules by the soft PLC. An additional software is needed for the more intelligent devises possessing complex technical functions, such as the control valve, the AC driver among others. The 4C control by the softing which is an important industrial solution for the soft PLC was specifically designed for the profibus. So, an OPC server is needed to exchange between the other fieldbuses while the optimum performance is only achieved by using the profibus card by softing. This implies that the business aspect has played a major role for developing the profibus by softing and the domestic users need to pay careful attention to this point additional software tools, such as the PDM or the comuwinn II, are necessary in the process control area to operate a complex and instruments using the DP/PA segment coupler. 13 refs., 46 figs., 12 tabs. (Author)

Measurement and control system for cryogenic helium gas bearing turbo-expander experimental platform based on Siemens PLC S7-300

Energy Technology Data Exchange (ETDEWEB)

Li, J.; Xiong, L. Y.; Peng, N.; Dong, B.; Liu, L. Q. [Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190 (China); Wang, P. [Beijing Sciample Technology Co., Ltd., Beijing, 100190 (China)

2014-01-29

An experimental platform for cryogenic Helium gas bearing turbo-expanders is established at the Technical Institute of Physics and Chemistry, Chinese Academy of Sciences. This turbo-expander experimental platform is designed for performance testing and experimental research on Helium turbo-expanders with different sizes from the liquid hydrogen temperature to the room temperature region. A measurement and control system based on Siemens PLC S7-300 for this turbo-expander experimental platform is developed. Proper sensors are selected to measure such parameters as temperature, pressure, rotation speed and air flow rate. All the collected data to be processed are transformed and transmitted to S7-300 CPU. Siemens S7-300 series PLC CPU315-2PN/DP is as master station and two sets of ET200M DP remote expand I/O is as slave station. Profibus-DP field communication is established between master station and slave stations. The upper computer Human Machine Interface (HMI) is compiled using Siemens configuration software WinCC V6.2. The upper computer communicates with PLC by means of industrial Ethernet. Centralized monitoring and distributed control is achieved. Experimental results show that this measurement and control system has fulfilled the test requirement for the turbo-expander experimental platform.

Measurement and control system for cryogenic helium gas bearing turbo-expander experimental platform based on Siemens PLC S7-300

Science.gov (United States)

Li, J.; Xiong, L. Y.; Peng, N.; Dong, B.; Wang, P.; Liu, L. Q.

2014-01-01

An experimental platform for cryogenic Helium gas bearing turbo-expanders is established at the Technical Institute of Physics and Chemistry, Chinese Academy of Sciences. This turbo-expander experimental platform is designed for performance testing and experimental research on Helium turbo-expanders with different sizes from the liquid hydrogen temperature to the room temperature region. A measurement and control system based on Siemens PLC S7-300 for this turbo-expander experimental platform is developed. Proper sensors are selected to measure such parameters as temperature, pressure, rotation speed and air flow rate. All the collected data to be processed are transformed and transmitted to S7-300 CPU. Siemens S7-300 series PLC CPU315-2PN/DP is as master station and two sets of ET200M DP remote expand I/O is as slave station. Profibus-DP field communication is established between master station and slave stations. The upper computer Human Machine Interface (HMI) is compiled using Siemens configuration software WinCC V6.2. The upper computer communicates with PLC by means of industrial Ethernet. Centralized monitoring and distributed control is achieved. Experimental results show that this measurement and control system has fulfilled the test requirement for the turbo-expander experimental platform.

Institutional radioactive waste management in the Nuclear Research Institute Rez plc

International Nuclear Information System (INIS)

Kovarik, P.; Svoboda, K.; Podlaha, J.

2008-01-01

Nuclear research institute Rez, plc. (mentioned below as NRI) has had a dominant position in the area of the nuclear research and development in the Czech Republic, the Central and the Eastern Europe. Naturally, the radioactive waste management is an integral part of the nuclear industry, research and development. For that reason, there is Centre of the radioactive waste management (mentioned below as Centre) in the NRI. This Centre is engaged in the radioactive waste treatment, decontamination, characterisation, decommissioning and other relevant activities. This paper describes the system of technology and other information about institutional radioactive waste management in the NRI. (authors)

Data acquisition and control system with a programmable logic controller (PLC) for a pulsed chemical oxygen-iodine laser

Science.gov (United States)

Yu, Haijun; Li, Guofu; Duo, Liping; Jin, Yuqi; Wang, Jian; Sang, Fengting; Kang, Yuanfu; Li, Liucheng; Wang, Yuanhu; Tang, Shukai; Yu, Hongliang

2015-02-01

A user-friendly data acquisition and control system (DACS) for a pulsed chemical oxygen -iodine laser (PCOIL) has been developed. It is implemented by an industrial control computer，a PLC, and a distributed input/output (I/O) module, as well as the valve and transmitter. The system is capable of handling 200 analogue/digital channels for performing various operations such as on-line acquisition, display, safety measures and control of various valves. These operations are controlled either by control switches configured on a PC while not running or by a pre-determined sequence or timings during the run. The system is capable of real-time acquisition and on-line estimation of important diagnostic parameters for optimization of a PCOIL. The DACS system has been programmed using software programmable logic controller (PLC). Using this DACS, more than 200 runs were given performed successfully.

Úzkopásmová PLC komunikace s vysokou datovou rychlostí na bázi OFDM techniky

OpenAIRE

Csiba, Patrik

2015-01-01

Tato bakalářská práce se zabývá úzkopásmovou PLC komunikací. V práci je popisována historie, princip úzkopásmové komunikace a popis čtyř dostupných úzkopásmových standardů (PRIME, G3-PLC, G.hnem, IEEE P1901.2) s jejich porovnáním. V další části jsou popisována experimentální měření, která jsou prováděna v domácím prostředí za použití dostupných modemů TMDSPLCKIT od firmy Texas Instruments, MT39-L1-ver.R modemů od firmy ModemTec a EVALKITST7580-1 od firmy STMicroelectronics. This bachelor p...

Business strategy and the environment Tesco Plc's declining financial performance and underlying issues

OpenAIRE

Haddock-Millar, Julie; Rigby, Chris

2015-01-01

The case presents a teaching tool which requires students to: 1) analyze the financial performance of Tesco Plc over the last four years; 2) compare Tesco’s market position with key competitors; 3) identify and evaluate Tesco’s business strategy; 4) evaluate the causes of Tesco’s decline in performance; 5) develop recommendations to address declining performance; 6) identify and evaluate the Human Resource strategic role in addressing and supporting performance. The case is suitable for a bus...

Developing a PLC-friendly state machine model: lessons learned

Science.gov (United States)

Pessemier, Wim; Deconinck, Geert; Raskin, Gert; Saey, Philippe; Van Winckel, Hans

2014-07-01

Modern Programmable Logic Controllers (PLCs) have become an attractive platform for controlling real-time aspects of astronomical telescopes and instruments due to their increased versatility, performance and standardization. Likewise, vendor-neutral middleware technologies such as OPC Unified Architecture (OPC UA) have recently demonstrated that they can greatly facilitate the integration of these industrial platforms into the overall control system. Many practical questions arise, however, when building multi-tiered control systems that consist of PLCs for low level control, and conventional software and platforms for higher level control. How should the PLC software be structured, so that it can rely on well-known programming paradigms on the one hand, and be mapped to a well-organized OPC UA interface on the other hand? Which programming languages of the IEC 61131-3 standard closely match the problem domains of the abstraction levels within this structure? How can the recent additions to the standard (such as the support for namespaces and object-oriented extensions) facilitate a model based development approach? To what degree can our applications already take advantage of the more advanced parts of the OPC UA standard, such as the high expressiveness of the semantic modeling language that it defines, or the support for events, aggregation of data, automatic discovery, ... ? What are the timing and concurrency problems to be expected for the higher level tiers of the control system due to the cyclic execution of control and communication tasks by the PLCs? We try to answer these questions by demonstrating a semantic state machine model that can readily be implemented using IEC 61131 and OPC UA. One that does not aim to capture all possible states of a system, but rather one that attempts to organize the course-grained structure and behaviour of a system. In this paper we focus on the intricacies of this seemingly simple task, and on the lessons that we

75 FR 50877 - Airworthiness Directives; Rolls-Royce plc RB211-524C2 Series Turbofan Engines

Science.gov (United States)

2010-08-18

... Airworthiness Directives; Rolls-Royce plc RB211-524C2 Series Turbofan Engines AGENCY: Federal Aviation... require adopting the AD as proposed. Costs of Compliance Based on the service information, we estimate.... Required parts will cost about $25,000 per product. Based on these figures, we estimate the cost of the AD...

78 FR 23316 - The Royal Bank of Scotland plc, et al.; Notice of Application and Temporary Order

Science.gov (United States)

2013-04-18

... plc, et al.; Notice of Application and Temporary Order April 12, 2013. AGENCY: Securities and Exchange Commission (``Commission''). ACTION: Temporary order and notice of application for a permanent order under... application for a permanent order. Applicants have also applied for a permanent order. APPLICANTS: The Royal...

Novel Networked Remote Laboratory Architecture for Open Connectivity Based on PLC-OPC-LabVIEW-EJS Integration. Application in Remote Fuzzy Control and Sensors Data Acquisition.

Science.gov (United States)

González, Isaías; Calderón, Antonio José; Mejías, Andrés; Andújar, José Manuel

2016-10-31

In this paper the design and implementation of a network for integrating Programmable Logic Controllers (PLC), the Object-Linking and Embedding for Process Control protocol (OPC) and the open-source Easy Java Simulations (EJS) package is presented. A LabVIEW interface and the Java-Internet-LabVIEW (JIL) server complete the scheme for data exchange. This configuration allows the user to remotely interact with the PLC. Such integration can be considered a novelty in scientific literature for remote control and sensor data acquisition of industrial plants. An experimental application devoted to remote laboratories is developed to demonstrate the feasibility and benefits of the proposed approach. The experiment to be conducted is the parameterization and supervision of a fuzzy controller of a DC servomotor. The graphical user interface has been developed with EJS and the fuzzy control is carried out by our own PLC. In fact, the distinctive features of the proposed novel network application are the integration of the OPC protocol to share information with the PLC and the application under control. The user can perform the tuning of the controller parameters online and observe in real time the effect on the servomotor behavior. The target group is engineering remote users, specifically in control- and automation-related tasks. The proposed architecture system is described and experimental results are presented.

Novel Networked Remote Laboratory Architecture for Open Connectivity Based on PLC-OPC-LabVIEW-EJS Integration. Application in Remote Fuzzy Control and Sensors Data Acquisition

Directory of Open Access Journals (Sweden)

Isaías González

2016-10-01

Full Text Available In this paper the design and implementation of a network for integrating Programmable Logic Controllers (PLC, the Object-Linking and Embedding for Process Control protocol (OPC and the open-source Easy Java Simulations (EJS package is presented. A LabVIEW interface and the Java-Internet-LabVIEW (JIL server complete the scheme for data exchange. This configuration allows the user to remotely interact with the PLC. Such integration can be considered a novelty in scientific literature for remote control and sensor data acquisition of industrial plants. An experimental application devoted to remote laboratories is developed to demonstrate the feasibility and benefits of the proposed approach. The experiment to be conducted is the parameterization and supervision of a fuzzy controller of a DC servomotor. The graphical user interface has been developed with EJS and the fuzzy control is carried out by our own PLC. In fact, the distinctive features of the proposed novel network application are the integration of the OPC protocol to share information with the PLC and the application under control. The user can perform the tuning of the controller parameters online and observe in real time the effect on the servomotor behavior. The target group is engineering remote users, specifically in control- and automation-related tasks. The proposed architecture system is described and experimental results are presented.

Novel Networked Remote Laboratory Architecture for Open Connectivity Based on PLC-OPC-LabVIEW-EJS Integration. Application in Remote Fuzzy Control and Sensors Data Acquisition

Science.gov (United States)

González, Isaías; Calderón, Antonio José; Mejías, Andrés; Andújar, José Manuel

2016-01-01

In this paper the design and implementation of a network for integrating Programmable Logic Controllers (PLC), the Object-Linking and Embedding for Process Control protocol (OPC) and the open-source Easy Java Simulations (EJS) package is presented. A LabVIEW interface and the Java-Internet-LabVIEW (JIL) server complete the scheme for data exchange. This configuration allows the user to remotely interact with the PLC. Such integration can be considered a novelty in scientific literature for remote control and sensor data acquisition of industrial plants. An experimental application devoted to remote laboratories is developed to demonstrate the feasibility and benefits of the proposed approach. The experiment to be conducted is the parameterization and supervision of a fuzzy controller of a DC servomotor. The graphical user interface has been developed with EJS and the fuzzy control is carried out by our own PLC. In fact, the distinctive features of the proposed novel network application are the integration of the OPC protocol to share information with the PLC and the application under control. The user can perform the tuning of the controller parameters online and observe in real time the effect on the servomotor behavior. The target group is engineering remote users, specifically in control- and automation-related tasks. The proposed architecture system is described and experimental results are presented. PMID:27809229

First Experience with PLC, OPC and BridgeVIEW in the context of the HMPID liquid distribution prototype

CERN Document Server

Maatta, E; CERN. Geneva; Swoboda, Detlef; Lecoeur, G

1999-01-01

The TESt (Test and Evaluation Station) [3] project consists in the construction of a stand alone unit for a specific sub-system of an ALICE detector in order to gain first experience with commercial products for detector control. Although the control system includes only a small number of devices and is designed for a particular application, it covers nevertheless all layers of a complete system and can be extended or used in different applications. The control system prototype has been implemented for the Perfluorohexane (C6F14) liquid distribution of the ALICE-HMPID (High-Momentum Particle IDentification)[5]. This report presents the experience acquired while developing the control system with off the shelf items: National Instruments BridgeVIEW [6] supervision software, SIEMENS PLC S7-300 [7] and the programming tool STEP 7. The OPC standard (OLE [12] for Process Control) [8] was used for the communication between BridgeVIEW and the PLC.

Real time control “es-dawet” mixer using dasboard based on PLC and WSN

Science.gov (United States)

Siagian, Pandapotan; Hutauruk, Sindak; Kisno

2017-09-01

The aim of this study is to monitor and acquire the remote parameters like Speed control a DC Motor, IR Sensor, Temperature of pasteurize mix of ice cream, and send these real values over wireless network. A proposed system is dashboard monitoring system for PLC based system wirelessly using ZigBee protocol. To implement this a ZigBee model is connected to a programmed digital signal controller which would transmit the data to Zigbee coordinator which is connected to a PC through RS232 serial communication. Person can need only to send the reply about the process that is to be carried out and PLC will check the status of the web base sent by person and take the action according to it where wired communication is either more expensive or impossible due to physical conditions. A low cost system for measured the parameters of motor such as IR Sensor, Speed control a DC Motor by PWM and temperature with Zigbee protocol connectivity. A database is built to execute monitoring and to save the motor parameters received by radio frequency (RF) data acquisition system. Experimental results show that the proposed system is less costly, provides higher accuracy as well as safe and gives visual environment.

75 FR 28655 - Rexam Closure Systems, Inc. a Subsidiary of Rexam PLC Including On-Site Leased Workers From...

Science.gov (United States)

2010-05-21

... Hamlet, NC; Amended Certification Regarding Eligibility To Apply for Worker Adjustment Assistance In..., applicable to the workers of Rexam Closure Systems, Inc., a subsidiary of Rexam PLC, Hamlet, North Carolina... paid through Owens Illinois Manufacturing, Hamlet, North Carolina, who became totally or partially...

Based on the Hardware Resources Configurable Shanke PLC Building Energy Consumption Detection System

Directory of Open Access Journals (Sweden)

Cheng Guanghe

2017-01-01

Full Text Available According to the actual situation of the comprehensive office building and the functional requirements of the building energy consumption monitoring and management system, the office building energy consumption monitoring and management system is designed by using the hardware resource configurable Shanke PLC(SKPLC as the data collector. The system uses data bus technology and field data acquisition technology to achieve the building energy consumption data acquisition and management. Practice has proved that energy-saving effect is good.

A systematic review of the effectiveness of smartphone applications that encourage dietary self-regulatory strategies for weight loss in overweight and obese adults.

Science.gov (United States)

Semper, H M; Povey, R; Clark-Carter, D

2016-09-01

The aim of this paper is to systematically review the evidence to explore whether smartphone applications that use self-regulatory strategies are beneficial for weight loss in overweight and obese adults over the age of 18 years. Sixteen electronic databases were searched for articles published up to April 2015 including MEDLINE, OVID, Ingenta, PSYCARTICLES and PSYCINFO, CINAHL, Sportdiscus, Science Direct, Web of Knowledge, Cochrane Library, JSTOR, EBSCO, Proquest, Wiley and Google Scholar. Twenty nine eligible studies were retrieved of which six studies met the inclusion criteria. Studies that recruited participants under the age of 18 years, adults with a chronic condition or did not report weight loss outcomes were excluded. Study findings were combined using a narrative synthesis. Overall, evidence suggests that smartphone applications may be a useful tool for self-regulating diet for weight loss as participants in the smartphone application group in all studies lost at least some bodyweight. However, when compared to other self-monitoring methods, there was no significant difference in the amount of weight lost. Findings should be interpreted with caution based on the design of the studies and the comparator groups used. Future research needs to be more methodologically rigorous and incorporate measures of whether eating habits become healthier in addition to measuring weight and BMI. © 2016 World Obesity.

160 Gb/s OFDM transmission utilizing an all-optical symbol generator based on PLC

Science.gov (United States)

Liang, Xiaojun; Qiao, Yaojun; Li, Wei; Mei, Junyao; Qin, Yi

2009-11-01

We demonstrate a 160 Gb/s orthogonal frequency division multiplexing (OFDM) system using an all-optical symbol generator based on planar light circuit (PLC) technology. Excellent bit error rate (BER) is observed after long-distance transmission. The proposed symbol generator fundamentally eliminates the processing speed limits introduced by electronics and is suitable for high integration, making it physically realizable to build high-speed all-optical OFDM systems with a large number of subcarriers.

Comparative response time and fault logging with a PLC and supervisory software and a standalone unit developed for recording

International Nuclear Information System (INIS)

Baldaconi, Ricardo H.; Costa, Fabio E. da

2017-01-01

The Cobalt-60 irradiator of IPEN / CNEN, a category IV facility, has a security system for inter locking doors or exposure of radioactive sources made simultaneously by a programmable logic controller (PLC) model S7-200 from Siemens and a relay logic. From a set of information, both systems work together opening doors or exposing the sources. All incoming and outgoing information are sent serially via EIA232 communication to a personal computer with Windows® platform for a supervisory program which provides the monitoring of the entire process by a synoptic table on the computer screen and is also intended to keep records of all events on the computer's hard drive. A deficiency was found for the process of sending events via serial communication (EIA232) from PLC to the supervisory program. When failure occurred in a very short time, the PLC always took the right decision, but the registration process that had to go through the Windows® timeshare lost the information. In the previous work developed a standalone electronics unit connected to the inputs and outputs of the security system, fully optocoupled to avoid any interference to the security system that records each event on a memory card. In this work, for checking the unit developed record time ability, transients incoming signals for simulating failures, were injected at security system inputs and the response time of security system, supervisory program and the autonomous unity were measured and compared. (author)

Comparative response time and fault logging with a PLC and supervisory software and a standalone unit developed for recording

Energy Technology Data Exchange (ETDEWEB)

Baldaconi, Ricardo H., E-mail: ricardohovacker@hotmail.com [Escola Senai Roberto Simonsen, Educação e Tecnologia, Sao Paulo, SP (Brazil); Costa, Fabio E. da, E-mail: fecosta@ipen.br [Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP), São Paulo, SP (Brazil)

2017-07-01

The Cobalt-60 irradiator of IPEN / CNEN, a category IV facility, has a security system for inter locking doors or exposure of radioactive sources made simultaneously by a programmable logic controller (PLC) model S7-200 from Siemens and a relay logic. From a set of information, both systems work together opening doors or exposing the sources. All incoming and outgoing information are sent serially via EIA232 communication to a personal computer with Windows® platform for a supervisory program which provides the monitoring of the entire process by a synoptic table on the computer screen and is also intended to keep records of all events on the computer's hard drive. A deficiency was found for the process of sending events via serial communication (EIA232) from PLC to the supervisory program. When failure occurred in a very short time, the PLC always took the right decision, but the registration process that had to go through the Windows® timeshare lost the information. In the previous work developed a standalone electronics unit connected to the inputs and outputs of the security system, fully optocoupled to avoid any interference to the security system that records each event on a memory card. In this work, for checking the unit developed record time ability, transients incoming signals for simulating failures, were injected at security system inputs and the response time of security system, supervisory program and the autonomous unity were measured and compared. (author)

The PLC-based Industrial Temperature Control System: Design and Implementation

Directory of Open Access Journals (Sweden)

Wei Fanjie

2017-01-01

Full Text Available Targeting at the problem of slow response and low accuracy of the automatic temperature control system for material processing and boiler heating, a new design method is proposed to work with the PLC-based temperature control system, where the box temperature control may be achieved through the fan and the heating plate. The hardware design and software design of the system are analyzed in detail. In this paper, a combination of the traditional PID control and the more popular fuzzy control is taken as the control program to achieve the overall design of the control algorithm. Followed by the simulation in the MATLAB software, the designed system is highlighted by its the characteristics of impressive stability, precision and robustness.

Development of multifunctional radiation monitoring instrument based on PLC technology

International Nuclear Information System (INIS)

Li Ziqiang; Zhu Yuye; Zhuang Min

2007-01-01

This eight-channel multifunctional Radiation Monitoring Instrument is developed by making use of the built-in high-speed counters and the powerful instruction system of the SIEMES SIMATICS S7 series Programmable Logic Controllers (PLC) to record and process the pulse signal output by the detectors. The instrument with functions, such as analog and digital display, digital storage of digital data, pulse signal generator, network communication, can connect various types of pulse detectors. The initial process can be translated between Graduation Apparatus method and Formula method. the logicality of the high-dosage warning system is processed itself. The signal output will drive the alarm lights and bell directly. This paper mainly describes the configuration, programming and feature of the instrument. (authors)

British Nuclear Fuels PLC: report and accounts 1989-90

International Nuclear Information System (INIS)

1991-01-01

Members of the Energy Select Committee put questions to representatives of British Nuclear Fuels PLC (BNFL) about the annual report and accounts 1989-90. Questions concerned the late publication of the accounts, BNFLs role in the downfall of the nuclear privatisation, government assistance to the nuclear industry, the price BNFL charges for fuel reprocessing and the process of laser isotope separation of uranium. The committee also asked about the rate of return in BNFL's assets as a percentage, Sellafield's potential as a site for a deep repository for radioactive wastes and the 6000 boreholes that MREX will drill at Sellafield. The commercial case for reprocessing is made. Feasibility studies for possible new reactions at Sellafield and Chapel Cross have been carried out. On the whole the Energy Committee were satisfied with the replies from BNFL. (UK)

MHC class I signaling in T cells leads to tyrosine kinase activity and PLC-gamma 1 phosphorylation

DEFF Research Database (Denmark)

Skov, S; Odum, Niels; Claesson, M H

1995-01-01

phosphorylation and the subsequent calcium response. The early tyrosine kinase activity was found to be dependent on expression of the TCR/CD3 complex and the CD45 molecule on the surface of the T cells. Furthermore, MHC-I cross-linking was shown to tyrosine phosphorylate PLC-gamma 1 (phospholipase C-gamma 1...

VirPLC: a Methodology to Developing Capacities, Skills and Self-esteem by the Logical Stimulus with a Simple, Functional and Dynamic Tool

Directory of Open Access Journals (Sweden)

Antoni FERRER ROJAS

2017-07-01

Full Text Available The proliferation of programming environments such as Logo, Minecraft, Code or Scratch is a consequence of the effectiveness of a graphic language for the introduction of students in programming but, some of the newer systems, such as App Inventor, are slow in the basic interaction: “make it” / “test it”. I propose an alternative for students from the age of 15. It is called VirPLC and it is oriented to something as functional as the systems control, through two screens: one with software to program and, another one with animated hardware to simulate it. VirPLC does not pretend to turn the user into an expert in automation, but to facilitate a first contact between the student and the logic world, by posing problems in practical, near and real control systems such as: crane control; TV competition; alarm; supermarket door; garage door; traffic light; lift... The student raises both the logical operativity, as well as the hardware requirements (inputs and outputs. The software works in an evolutionary and repeated way: “make it” / “test it” and consolidate to improve it. It allows the evolution in levels of greater complexity, where it is debugged until acquiring a solid “product”, functional, safe, versatile and installable. VirPLC step by step “hooks” students who pursue challenges and offers an alternative to apply after some Boolean Algebra notions, and before written programming, with objects, events and classes. VirPLC is freeware, it works under Windows (from XP to W10 and can be downloaded from the author’s WEB. At install time, it adds a folder with more than 30 examples often deliberately incomplete, along with a mini-course with several proposed practices.

Changes in Teachers' Beliefs and Classroom Practices Concerning Inquiry-Based Instruction Following a Year-Long RET-PLC Program

Science.gov (United States)

Miranda, Rommel J.; Damico, Julie B.

2015-01-01

This mixed-methods study examines how engaging science teachers in a summer Research Experiences for Teachers (RET) followed by an academic-year Professional Learning Community (PLC) focused on translating teacher research experiences to inquiry-based classroom lessons might facilitate changes in their beliefs and classroom practices regarding…

A water pumping control system with a programmable logic controller (PLC) and industrial wireless modules for industrial plants--an experimental setup.

Science.gov (United States)

Bayindir, Ramazan; Cetinceviz, Yucel

2011-04-01

This paper describes a water pumping control system that is designed for production plants and implemented in an experimental setup in a laboratory. These plants contain harsh environments in which chemicals, vibrations or moving parts exist that could potentially damage the cabling or wires that are part of the control system. Furthermore, the data has to be transferred over paths that are accessible to the public. The control systems that it uses are a programmable logic controller (PLC) and industrial wireless local area network (IWLAN) technologies. It is implemented by a PLC, an communication processor (CP), two IWLAN modules, and a distributed input/output (I/O) module, as well as the water pump and sensors. Our system communication is based on an Industrial Ethernet and uses the standard Transport Control Protocol/Internet Protocol for parameterisation, configuration and diagnostics. The main function of the PLC is to send a digital signal to the water pump to turn it on or off, based on the tank level, using a pressure transmitter and inputs from limit switches that indicate the level of the water in the tank. This paper aims to provide a convenient solution in process plants where cabling is not possible. It also has lower installation and maintenance cost, provides reliable operation, and robust and flexible construction, suitable for industrial applications. Copyright © 2010 ISA. Published by Elsevier Ltd. All rights reserved.

Synchronization in a PLC/VAX-based control and data-acquisition system of a nuclear-fusion experiment

International Nuclear Information System (INIS)

Schmidt, V.; Flor, G.; Hemming, O.N.; Luchetta, A.; Manduchi, G.; Vitturi, S.

1990-01-01

This paper describes the concept and implementation details of the synchronization mechanisms used in the control and data-acquisition system of the RFX (Reversed-Field Experiment) nuclear-fusion experimental device, at present under construction in Padova, Italy, within the framework of the co-ordinated nuclear-fusion research programme of the European Communities. The system employs industrial PLCs for the 'slow' control and monitoring functions, and a VAX-based CAMAC for the 'fast' functions of trigger-signal generation and data acquisition during the experiment pulses. All subsystems communicate via Ethernet, using compatible software protocols. The operational sequence of the complete system is governed by a single state machine implemented on a PLC-based supervisor system. Equivalent 'slave' state machines are implemented on all other subsystems (PLC- and VAX-based). These state machines are synchronized by means of the exchange of messages via Ethernet. This paper deals in detail with the following components which are involved in system synchronization: The Message Exchange System which implements the system-wide exchange of short messages; the Scheduler programs which implement the state machine on the various computing nodes, and which make use of the Message Exchange System. (orig.)

Quality of Non-Interest Banking Services and Customers’ Satisfaction: Evidence from Jaiz Bank PLC, Kaduna, Nigeria

Directory of Open Access Journals (Sweden)

Yusuf Ismaila Mustapha

2017-02-01

Full Text Available The dwindling levels of service quality in Nigerian financial institutions had resulted in abysmal performance of most banks and other financial institutions in the sector and this challenge has made it imperative for financial firms to be customer centered in all their operational activities. The purpose of this study is to determine the effect of service quality of non-interest bank on customers’ satisfaction using Jaiz Bank PLC, Kaduna as a case study. The study utilized convenience and snowball sampling techniques to select 147 customers who served as respondents in the study. Correlation and multiple regression analysis were employed as analytical techniques. The results show that compliance with Islamic laws is highly correlated with customer satisfaction which was followed respectively by assurance, reliability, tangibility, responsiveness and empathy dimensions. It was also discovered that the six service quality dimensions significantly influenced the satisfaction of Jaiz Bank Plc, Kaduna branch customers’. The study recommends that management of the bank should exploit and maneuver the service quality dimensions as a survival strategy for improving customers’ satisfaction in an industry characterized by intense competition and environmental dynamism.

PEMBUATAN SISTEM KONTROL MESIN CAULKING ROD GUIDE OTOMATIS MENGGUNAKAN PLC OMRON CPM1A

Directory of Open Access Journals (Sweden)

Syahril Ardi

2015-02-01

Full Text Available Kenaikan tingkat produksi shock absorber perusahaan manufaktur membuat proses produksi harus berjalan dengan baik . Pipa proses pemotongan pada mendempul panduan mesin batang memiliki beberapa masalah yang harus diatasi . Untuk mengatasi masalah tersebut kemudian melakukan modifikasi mesin batang mendempul yang secara otomatis dapat mengurangi waktu siklus pada proses mendempul batang dari 4,5 detik per potong sehingga tingkat produksi memotong garis pipa dapat ditingkatkan . Sistem kontrol otomatis mendempul mesin batang panduan ini dirancang untuk menggunakan Programmable Logic Controller (PLC

A Component-Based Modeling and Validation Method for PLC Systems

Directory of Open Access Journals (Sweden)

Rui Wang

2014-05-01

Full Text Available Programmable logic controllers (PLCs are complex embedded systems that are widely used in industry. This paper presents a component-based modeling and validation method for PLC systems using the behavior-interaction-priority (BIP framework. We designed a general system architecture and a component library for a type of device control system. The control software and hardware of the environment were all modeled as BIP components. System requirements were formalized as monitors. Simulation was carried out to validate the system model. A realistic example from industry of the gates control system was employed to illustrate our strategies. We found a couple of design errors during the simulation, which helped us to improve the dependability of the original systems. The results of experiment demonstrated the effectiveness of our approach.

Hukum Lingkungan dan Pertanggungjawaban Strict Liability dalam Sistem Hukum Common Law (Studi Kasus Cambridge Water Co. Ltd v. Eastern Countries Leather Plc

Directory of Open Access Journals (Sweden)

Mahfud Mahfud

2010-12-01

Full Text Available ABSTRACT: The possibilities for pollution control still offered today Blackburn J.’s celebrated rule of strict liability, now almost 130 years old, has ensured its continuing popularity. There can be few tort lawyers, however, who have not increasingly wondered how much time should be devoted to a case which has received little judicial attention in recent years, and which was last subjected to detailed consideration by the House of Lords nearly 50 years ago, until, that is, the much-published decision of the House of Lords in Cambridge Water Co. Ltd v. Eastern Countries Leather Plc.   (Environmental Law and The Strict Liability Application In the Common Law System (The Case Study of Cambridge Water Co. Ltd V. Eastern Countries Leather Plc

Software design specification and analysis(NuFDS) approach for the safety critical software based on porgrammable logic controller(PLC)

International Nuclear Information System (INIS)

Koo, Seo Ryong; Seong, Poong Hyun; Jung, Jin Yong; Choi, Seong Soo

2004-01-01

This paper introduces the software design specification and analysis technique for the safety-critical system based on Programmable Logic Controller (PLC). During software development phases, the design phase should perform an important role to connect between requirements phase and implementation phase as a process of translating problem requirements into software structures. In this work, the Nuclear FBD-style Design Specification and analysis (NuFDS) approach was proposed. The NuFDS approach for nuclear Instrumentation and Control (I and C) software are suggested in a straight forward manner. It consists of four major specifications as follows; Database, Software Architecture, System Behavior, and PLC Hardware Configuration. Additionally, correctness, completeness, consistency, and traceability check techniques are also suggested for the formal design analysis in NuFDS approach. In addition, for the tool supporting, we are developing NuSDS tool based on the NuFDS approach which is a tool, especially for the software design specification in nuclear fields

76 FR 24793 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-524 Series and RB211 Trent 500, 700, and 800...

Science.gov (United States)

2011-05-03

... Airworthiness Directives; Rolls-Royce plc (RR) RB211-524 Series and RB211 Trent 500, 700, and 800 Series... the AD. Costs of Compliance Based on the service information, we estimate that this AD would affect...

75 FR 58290 - Airworthiness Directives; Rolls-Royce plc RB211 Trent 700 and Trent 800 Series Turbofan Engines

Science.gov (United States)

2010-09-24

... Airworthiness Directives; Rolls-Royce plc RB211 Trent 700 and Trent 800 Series Turbofan Engines AGENCY: Federal... or increase the scope of the AD. Costs of Compliance Based on the service information, we estimate.... Required parts would cost about $15,000 per product. Based on these figures, we estimate the cost of the AD...

Design and construction of automatic operating system of double chamber plasma nitriding device PLC based

International Nuclear Information System (INIS)

Saminto; Slamet Santosa; Eko Priyono

2012-01-01

The automatic operating system of double chamber plasma nitriding device has been done. The system is used for operating double chamber plasma nitriding automatically as according to the standard operating procedure by pressing push button on the human machine interface (HMI). The system consists of hardware and software. The hardware was constructed using main components T100MD1616+ PLC module and supported by temperature signal conditioner module, Wheatstone bridge module, isolated amplifier module and EMS 30A H Bridge motor driver module. A software program that is planted on T100MD1616+ PLC using ladder diagrams and Tbasic program. Test system functions performed by inserting a set values of temperature and pressure by pressing the button on the human machine interface (HMI). The test results show that the temperature control with a set of values 100 °C obtained stable coverage of 98 °C to 102 °C, (Δ ± 2 °C) with a 2% tolerance and the output voltage of the DAC is 2.436 volts to 2.913 volts. The pressure control with a set of values 2.169 x 10 -1 mbar obtained stable coverage of 1.995 x 10 -1 mbar to 2.205 x 10 -1 mbar, (Δ ± 0.105 x 10 -1 mbar) with a 5% tol. (author)

The Interface Between Redundant Processor Modules Of Safety Grade PLC Using Mass Storage DPRAM

International Nuclear Information System (INIS)

Hwang, Sung Jae; Song, Seong Hwan; No, Young Hun; Yun, Dong Hwa; Park, Gang Min; Kim, Min Gyu; Choi, Kyung Chul; Lee, Ui Taek

2010-01-01

Processor module of safety grade PLC (hereinafter called as POSAFE-Q) developed by POSCO ICT provides high reliability and safety. However, POSAFEQ would have suffered a malfunction when we think taking place of abnormal operation by exceptional environmental. POSAFE-Q would not able to conduct its function normally in such case. To prevent these situations, the necessity of redundant processor module has been raised. Therefore, redundant processor module, NCPU-2Q, has been developed which has not only functions of single processor module with high reliability and safety but also functions of redundant processor

Aplicación de la Tecnología PLC en la provincia del Azuay

OpenAIRE

Jara Guapisaca, Claudio Xavier

2006-01-01

La Red PLC utiliza infraestructura ya instalada para atender a un mercado masivo, siendo una alternativa eficiente en términos de requerimientos de capital y tiempos de ejecución. La técnica es bastante sencilla de entender; consiste en acondicionar parte de las actuales infraestructuras eléctricas para que puedan transmitir señales regulares de baja frecuencia y otras por encima de la banda de 1 MHz, sin que se vea afectado el rendimiento eléctrico. Las señales de baja frecuencia (50 ó 60...

Capital Discipline and Financial Market Relations in Retail Globalization: Insights from the Case of Tesco plc

OpenAIRE

Wood, Steven; Wrigley, N; Coe, NM

2016-01-01

This paper provides an in-depth study of leading transnational food retailer Tesco plc to explore how its financial management and relations with the investment community – notably its reputation for capital discipline – underpinned successful expansion. Informed by close dialogue with equity analysts, we investigate how this model deteriorated since the late 2000s with declining returns, leading to high-profile international divestitures. The analysis assesses the drivers of these difficulti...

Design and realization of the waster level system based on PLC and configuration technology

International Nuclear Information System (INIS)

Hu Kaiming; Ge Yuanxiang; Fu Zhijian

2012-01-01

The process control experimental system takes the water level as the controlled object. In this system PLC is adopted as a controller and the electric valve for the actuator. Based on the water level system in the establishment of the mathematical model, the system uses PID algorithm, the PID parameters are tuning, MCGS configuration environment is built and debugs in the environment. Experiments show that the system is running smoothly, the overshoot is 18%, the setting time is about 3 min. The control indicators have reached the engineering requirements and the control effect is good. (authors)

The Application of S7-400H redundant PLC in I and C system for waterworks in nuclear power plant

International Nuclear Information System (INIS)

Pang Yuxiang

2013-01-01

This paper introduces Siemens S7-400H redundant PLC which is employed to implement monitor and control systems for waterworks in nuclear power plant. It focuses on the configuration and realization of the redundant system. The safety and reliability of the system is improved by using redundant CPU, network and server. (authors)

Application of hepatitis B core particles produced by human primary hepatocellular carcinoma (PLC/342) propagated in nude mice to the determination of anti-HBc by passive hemagglutination.

Science.gov (United States)

Miyamoto, K; Itoh, Y; Tsuda, F; Matsui, T; Tanaka, T; Miyamoto, H; Naitoh, S; Imai, M; Usuda, S; Nakamura, T

1986-05-22

Human primary hepatocellular carcinoma (PLC/342), carried by nude mice, produces hepatitis B core particles as well as hepatitis B surface antigen particles. Core particles purified form PLC/342 tumors displayed epitopes of hepatitis B core antigen (HBcAg) but not epitopes of hepatitis B e antigen (HBeAg) on their surface, unlike core particles prepared from Dane particles, derived from plasma of asymptomatic carriers, that expressed epitopes of both HBcAg and HBeAg. Core particles obtained from PLC/342 tumors were applied to the determination of antibody to HBcAg (anti-HBc) by passive hemagglutination. The assay detected anti-HBc not only in individuals with persistent infection with hepatitis B virus and in those who had recovered from transient infection, but also in patients with acute type B hepatitis, indicating that it can detect anti-HBc of either IgG or IgM class. A liberal availability of core particles from tumors carried by nude mice, taken together with an easy applicability of the method, would make the passive hemagglutination for anti-HBc a valuable tool in clinical and epidemiological studies, especially in places where sophisticated methods are not feasible.

Pancreatic acini possess endothelin receptors whose internalization is regulated by PLC-activating agents.

Science.gov (United States)

Hildebrand, P; Mrozinski, J E; Mantey, S A; Patto, R J; Jensen, R T

1993-05-01

Endothelin-1 (ET-1) and ET-3 mRNA have been found in the pancreas. We investigated the ability of ET-1, ET-2, and ET-3 to interact with and alter dispersed rat pancreatic acinar cell function. Radiolabeled ETs bound in a time- and temperature-dependent fashion, which was specific and saturable. Analysis demonstrated two classes of receptors, one class (ETA receptor) had a high affinity for ET-1 but a low affinity for ET-3, and the other class (ETB receptor) had equally high affinities for ET-1 and ET-3. No specific receptor for ET-2 was identified. Pancreatic secretagogues that activate phospholipase C (PLC) inhibited binding of 125I-labeled ET-1 (125I-ET-1) or 125I-ET-3, whereas agents that act through adenosine 3',5'-cyclic monophosphate (cAMP) did not. A23187 had no effect on 125I-ET-1 or 125I-ET-3 binding, whereas the phorbol ester 12-O-tetradecanoylphorbol 13-acetate reduced binding. The effect of cholecystokinin octapeptide (CCK-8) was mediated through its own receptor. Stripping of surface bound ligand studies demonstrated that both 125I-labeled ET-1 and 125I-labeled ET-3 were rapidly internalized. CCK-8 decreased the internalization but did not change the amount of surface bound ligand. Endothelins neither stimulate nor alter changes in enzyme secretion, intracellular calcium, cAMP, or [3H]inositol trisphosphate (IP3). This study demonstrates the presence of ETA and ETB receptors on rat pancreatic acini; occupation of both receptors resulted in rapid internalization, which is regulated by PLC-activating secretagogues. Occupation of either ET receptor did not alter intracellular calcium, cAMP, IP3, or stimulate amylase release.

WenTong HuoXue Cream Can Inhibit the Reduction of the Pain-Related Molecule PLC-β3 in the Dorsal Root Ganglion of a Rat Model of Diabetic Peripheral Neuropathy.

Science.gov (United States)

Feng, Chengcheng; Xu, Lijuan; Guo, Shiyun; Chen, Qian; Shen, Yuguo; Zang, Deng; Ma, Li

2018-01-01

WenTong HuoXue Cream (WTHX-Cream) has been shown to effectively alleviate clinical symptoms of diabetic peripheral neuropathy (DPN). This study investigated the gene and protein expression of the pain-related molecule PLC- β 3 in the dorsal root ganglion (DRG) of DPN rats. 88 specific pathogen-free male Wistar rats were randomly divided into placebo (10 rats) and DPN model (78 rats) groups, and the 78 model rats were used to establish the DPN model by intraperitoneal injection of streptozotocin and were then fed a high-fat diet for 8 weeks. These rats were randomly divided into the model group, the high-, medium-, and low-dose WTHX-Cream + metformin groups, the metformin group, the capsaicin cream group, and the capsaicin cream + metformin group. After 4 weeks of continuous drug administration, the blood glucose, body weight, behavioral indexes, and sciatic nerve conduction velocity were measured. The pathological structure of the DRG and the sciatic nerve were observed. PLC- β 3 mRNA and protein levels in the DRG of rats were measured. Compared with the model group, the high-dose WTHX-Cream group showed increased sciatic nerve conduction velocity, improved sciatic nerve morphological changes, and increased expression of PLC- β 3 mRNA and protein in the DRG. This study showed that WTHX-Cream improves hyperalgesia symptoms of DPN by inhibiting the reduction of PLC- β 3 mRNA and protein expression in the diabetic DRG of DPN rats.

WenTong HuoXue Cream Can Inhibit the Reduction of the Pain-Related Molecule PLC-β3 in the Dorsal Root Ganglion of a Rat Model of Diabetic Peripheral Neuropathy

Directory of Open Access Journals (Sweden)

Chengcheng Feng

2018-01-01

Full Text Available WenTong HuoXue Cream (WTHX-Cream has been shown to effectively alleviate clinical symptoms of diabetic peripheral neuropathy (DPN. This study investigated the gene and protein expression of the pain-related molecule PLC-β3 in the dorsal root ganglion (DRG of DPN rats. 88 specific pathogen-free male Wistar rats were randomly divided into placebo (10 rats and DPN model (78 rats groups, and the 78 model rats were used to establish the DPN model by intraperitoneal injection of streptozotocin and were then fed a high-fat diet for 8 weeks. These rats were randomly divided into the model group, the high-, medium-, and low-dose WTHX-Cream + metformin groups, the metformin group, the capsaicin cream group, and the capsaicin cream + metformin group. After 4 weeks of continuous drug administration, the blood glucose, body weight, behavioral indexes, and sciatic nerve conduction velocity were measured. The pathological structure of the DRG and the sciatic nerve were observed. PLC-β3 mRNA and protein levels in the DRG of rats were measured. Compared with the model group, the high-dose WTHX-Cream group showed increased sciatic nerve conduction velocity, improved sciatic nerve morphological changes, and increased expression of PLC-β3 mRNA and protein in the DRG. This study showed that WTHX-Cream improves hyperalgesia symptoms of DPN by inhibiting the reduction of PLC-β3 mRNA and protein expression in the diabetic DRG of DPN rats.

75 FR 51654 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-22B and RB211-524 Series Turbofan Engines

Science.gov (United States)

2010-08-23

... Airworthiness Directives; Rolls-Royce plc (RR) RB211-22B and RB211-524 Series Turbofan Engines AGENCY: Federal... that air safety and the public interest require adopting the AD as proposed. Costs of Compliance Based... labor rate is $85 per work-hour. Required parts will cost about $15,000 per product. Based on these...

Compact and high-sensitivity 100-Gb/s (4 × 25 Gb/s) APD-ROSA with a LAN-WDM PLC demultiplexer.

Science.gov (United States)

Yoshimatsu, Toshihide; Nada, Masahiro; Oguma, Manabu; Yokoyama, Haruki; Ohno, Tetsuichiro; Doi, Yoshiyuki; Ogawa, Ikuo; Takahashi, Hiroshi; Yoshida, Eiji

2012-12-10

We demonstrate an integrated 100 GbE receiver optical sub-assembly (ROSA) that incorporates a monolithic four-channel avalanche photodiode (APD) array and a planer lightwave circuit (PLC) based LAN-WDM demultiplexer. A record minimum receiver sensitivity of -20 dBm and 50-km error-free SMF transmission without an optical amplifier have been achieved.

76 FR 20229 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 768-60 and Trent 772-60 Turbofan Engines

Science.gov (United States)

2011-04-12

... inspections of the MCD. We are issuing this AD to prevent in-flight engine shutdowns caused by step aside... Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 768-60 and Trent 772-60 Turbofan Engines AGENCY... superseding an existing airworthiness directive (AD) for RR RB211-Trent 700 series turbofan engines. That AD...

Peptide microarray profiling identifies phospholipase C gamma 1 (PLC-γ1) as a potential target for t(8;21) AML

NARCIS (Netherlands)

Mahmud, Hasan; Scherpen, Frank J. G.; Meeuwsen de Boer, Tiny; Lourens, Harm-Jan; Schoenherr, Caroline; Eder, Matthias; Scherr, Michaela; Guryev, Victor; de Bont, Eveline S.

2017-01-01

The t(8;21) (q22;q22) chromosomal translocation is one of the most frequent genetic alterations in acute myeloid leukemia (AML) which has a need for improved therapeutic strategies. We found PLC-γ1 as one of the highest phosphorylated peptides in t(8;21) AML samples compared to NBM or CN-AML in our

STS-55 MS1/PLC Ross monitors Payload Specialist Walter's Anthrorack activity

Science.gov (United States)

1993-01-01

STS-55 German Payload Specialist 1 Ulrich Walter breathes into Rack 9 Anthrorack (AR) (Human Physiology Laboratory) device for Pulmonary Perfusion and Ventilation During Rest and Exercise experiment while working inside the Spacelab Deutsche 2 (SL-D2) science module aboard the Earth-orbiting Columbia, Orbiter Vehicle (OV) 102. Seated on the bicycle ergometer, Walter utilizes the respiratory monitoring system, part of a broad battery of experiments designed to investigate human physiology under microgravity conditions. In the background, Mission Specialist 1 (MS1) and Payload Commander (PLC) Jerry L. Ross monitors Walter's activity. Walter represents the German Aerospace Research Establishment (DLR) on the 10-day SL-D2 mission. Visible on the aft end cone are a fire extinguisher and the Crew Telesupport Experiment (CTE) Macintosh portable computer mounted on an adjustable work platform.

Safety culture development in nuclear electric plc

International Nuclear Information System (INIS)

Gibson, G.P.; Low, M.B.J.

1995-01-01

Nuclear Electric plc (NE) has always given the highest priority to safety. However, past emphasis has been directed towards ensuring safety thorough engineering design and hazard control procedures. Whilst the company did achieve high safety standards, particularly with respect to accidents, it was recognized that further improvements could be obtained. Analysis of the safety performance across a wide range of industries showed that the key to improving safety performance lay in developing a strong safety culture within the company. Over the last five years, NE has made great strides to improve its safety culture. This has resulted in a considerable improvement in its measured safety performance indicators, such as the number of incidents at international nuclear event scale (INES) rating 1, the number of lost time accidents and the collective radiation dose. However, despite this success, the company is committed to further improvement and a means by which this process becomes self-sustaining. In this way the company will achieve its prime goal, to ''ensure the safety of people, plant and the environment''. The paper provides an overview of the development of safety culture in NE since its formation in November 1989. It describes the research and international developments that have influenced the company's understanding of safety culture, the key initiatives that the company has undertaken to enhance its safety culture and the future initiatives being considered to ensure continual improvement. (author). 5 refs, 2 figs, 2 tabs

Implementation of RS-485 Communication between PLC and PC of Distributed Control System Based on VB

Science.gov (United States)

Lian Zhang, Chuan; Da Huang, Zhi; Qing Zhou, Gui; Chong, Kil To

2015-05-01

This paper focuses on achieving RS-485 communication between programmable logical controller (PLC) and PC based on visual basic 6.0 (VB6.0) on an experimental automatic production line. Mitsubishi FX2N PLCs and a PC are chosen as slave stations and main station, respectively. Monitoring software is developed using VB6.0 for data input/output, flow control and online parameters setting. As a result, all functions are fulfilled with robust performance. It is concluded from results that one PC can monitor several PLCs using RS-485 communication.

Design and Implementation of Control and Monitoring Systems Based on HMI-PLC for Potable Water Well

Directory of Open Access Journals (Sweden)

Quezada-Quezada José Carlos

2014-01-01

Full Text Available This project reports on the design and implementation in a workbench of a control and monitoring system of the discharge of water of a well. Graphic User's Interfaces (GUI are designed for interaction with the operator. The Human Machine Interface (HMI was implement in proprietor software and it contemplates the rules for control and monitoring of the conditions of the system for the operator, the HMI is also interconnected a Programmable Logic Controller (PLC where the rules of protection of the process are implemented in Ladder Diagram (LD.

Bacillus cereus Fnr binds a [4Fe-4S] cluster and forms a ternary complex with ResD and PlcR

Directory of Open Access Journals (Sweden)

Esbelin Julia

2012-06-01

Full Text Available Abstract Background Bacillus cereus is a facultative anaerobe that causes diarrheal disease in humans. Diarrheal syndrome may result from the secretion of various virulence factors including hemolysin BL and nonhemolytic enterotoxin Nhe. Expression of genes encoding Hbl and Nhe is regulated by the two redox systems, ResDE and Fnr, and the virulence regulator PlcR. B. cereus Fnr is a member of the Crp/Fnr family of iron-sulfur (Fe-S proteins. Only its apo-form has so far been studied. A major goal in deciphering the Fnr-dependent regulation of enterotoxin genes is thus to obtain and characterize holoFnr. Results Fnr has been subjected to in vitro Fe-S cluster reconstitution under anoxic conditions. UV-visible and EPR spectroscopic analyses together with the chemical estimation of the iron content indicated that Fnr binds one [4Fe-4S]2+ cluster per monomer. Atmospheric O2 causes disassembly of the Fe-S cluster, which exhibited a half-life of 15 min in air. Holo- and apoFnr have similar affinities for the nhe and hbl promoter regions, while holoFnr has a higher affinity for fnr promoter region than apoFnr. Both the apo- and holo-form of Fnr interact with ResD and PlcR to form a ternary complex. Conclusions Overall, this work shows that incorporation of the [4Fe-4S]2+ cluster is not required for DNA binding of Fnr to promoter regions of hbl and nhe enterotoxin genes or for the formation of a ternary complex with ResD and PlcR. This points to some new unusual properties of Fnr that may have physiological relevance in the redox regulation of enterotoxin gene regulation.

Decommissioning of the gaseous diffusion plant at BNF plc Capenhurst in the UK

International Nuclear Information System (INIS)

Clements, D.W.; Cross, J.R.

1993-01-01

Since 1982, a gaseous diffusion plant located at the British Nuclear Fuels plc (BNFL) site at Capenhurst in the United Kingdom, has been undergoing decontamination, decommissioning, and dismantling. By March 1994, the decontamination and decommissioning activities will be complete with 99% of the materials used to construct the plant recycled to the environment as clean material. This paper describes the history of the decontamination, decommissioning, dismantling, and disposal program. Reference is made to the scale of the project and to the special techniques developed, particularly in the areas of size reduction, decontamination, and protection of personnel and the environment. The quantities of material involved that require decontamination and release levels for recycling materials in the U.K. metals market are discussed

Users` demands narrow PLC-DCS gap

Energy Technology Data Exchange (ETDEWEB)

La Fauci, J.

1997-02-01

Supervisory control and data acquisition (SCADA) operator interface (OI) software has propelled programmable logic controllers (PLCs) into areas where they can successfully compete with distributed control systems (DCSs) for many control applications. As a result, automation engineers are struggling to develop guidelines to help determine which is best for batch operations and other applications. There is no clear answer to this issue. There are, however, decision tools such as Kepner-Tregoe (K-T) that can be applied by engineers as a structured approach to decision analysis and system selection. Other factors such as business environment, pressure to reduce project cost, validation, and predicting new technology direction all play a critical role for engineers in choosing between a PLC- or DCS-based control system. Higher-level business issues, however, are seldom considered by engineers during control system selection. Engineers should try to better understand their company`s business objectives and mission statement and how company business direction may affect control system selection. For instance, the pharmaceutical industry can be broken up into the following five basic application groups: bulk chemicals, finishing, biotech, pilot plant, and utilities. Each has a unique set of functional and process-control requirements. Understanding needs and differences of these five basic application groups and applying the optimum control system solution will place the company in a more competitive position. A financial analysis should be one of the first steps in the control system evaluation process. This may include early agreement of contractual terms and conditions as well as a nondisclosure agreement. Other financial considerations may include requesting a financial report on the control system manufacturer or systems integrator that will be performing the work to determine its financial stability. 3 figs.

Design of Measurement and Control System of Composite Fast Transfer Switch Based on PLC

Directory of Open Access Journals (Sweden)

ZHANG Hong-yi

2017-06-01

Full Text Available The fast transfer switch gets more extensive application in the power supply system along with the raising of power supply requirement for continuity and reliability in the sensitivity loads such as in airport，military place，hospital and large scale industrial production line． Therefore it is important that how to make fast transfer switch run safely and reliably． The paper expatiated the structure and principle of a fast transfer switch based on mechanical and electronic compound technology，and，according to the basic requirement and the characteristic of the fast transfer switch，a PLC mastered measuring and controlling system has been designed to raise the operation reliability of the fast transfer switch．

NuSEE: an integrated environment of software specification and V and V for PLC based safety-critical systems

International Nuclear Information System (INIS)

Koo, Seo Ryong; Seong, Poong Hyun; Yoo, Jun Beom; Cha, Sung Deok; Youn, Cheong; Han, Hyun Chul

2006-01-01

As the use of digital systems becomes more prevalent, adequate techniques for software specification and analysis have become increasingly important in Nuclear Power Plant (NPP) safety-critical systems. Additionally, the importance of software Verification and Validation (V and V) based on adequate specification has received greater emphasis in view of improving software quality. For thorough V and V of safety-critical systems, V and V should be performed throughout the software lifecycle. However, systematic V and V is difficult as it involves many manual-oriented tasks. Tool support is needed in order to more conveniently perform software V and V. In response, we developed four kinds of Computer Aided Software Engineering (CASE) tools to support system specification for a formal-based analysis according to the software lifecycle. In this work, we achieved optimized integration of each tool. The toolset, NuSEE, is an integrated environment for software specification and V and V for PLC based safety-critical systems. In accordance with the software lifecycle, NuSEE consists of NuSISRT for the concept phase, NuSRS for the requirements phase, NuSDS for the design phase and NuSCM for configuration management. It is believed that after further development our integrated environment will be a unique and promising software specification and analysis toolset that will support the entire software lifecycle for the development of PLC based NPP safety-critical systems

Fault tree synthesis for software design analysis of PLC based safety-critical systems

International Nuclear Information System (INIS)

Koo, S. R.; Cho, C. H.; Seong, P. H.

2006-01-01

As a software verification and validation should be performed for the development of PLC based safety-critical systems, a software safety analysis is also considered in line with entire software life cycle. In this paper, we propose a technique of software safety analysis in the design phase. Among various software hazard analysis techniques, fault tree analysis is most widely used for the safety analysis of nuclear power plant systems. Fault tree analysis also has the most intuitive notation and makes both qualitative and quantitative analyses possible. To analyze the design phase more effectively, we propose a technique of fault tree synthesis, along with a universal fault tree template for the architecture modules of nuclear software. Consequently, we can analyze the safety of software on the basis of fault tree synthesis. (authors)

STS-55 MS1/PLC Ross and Payload Specialist Walter work in SL-D2 module

Science.gov (United States)

1993-01-01

STS-55 Mission Specialist 1 (MS1) and Payload Commander (PLC) Jerry L. Ross floats near cycle ergometer and Rack 9 Anthrorack (AR) (Human Physiology Laboratory) as German Payload Specialist 1 Ulrich Walter reviews a checklist in front of Rack 11 Experiment Rack. These experiment stations and the crewmembers are in the shirt-sleeve environment of the Spacelab Deutsche 2 (SL-D2) science module onboard the Earth-orbiting Columbia, Orbiter Vehicle (OV) 102. In the background is the SL-D2 aft end cone. Behind Ross and Walter is Rack 12 Experiment Rack with Baroreflex (BA).

Developing Resilience Through the Modern Army Combatives Program

Science.gov (United States)

2014-05-22

Library online, as well as the SCIRUS, Psynet, PubMed, SagePub, Taylor and Francis, Ingenta Connect, Karger, JAMA Network, and Science Direct search...between Successful and Less Successful Karate Participants." Perceptual and Motor Skills 68, no. 2 (1989): 505– 506. Mills, Nigel , Janet Allen

Optical buffer based on monolithic InP phased-array 1×16 switch with silica-PLC pitch converter and ultra-compact coiled fiber delay lines

NARCIS (Netherlands)

Tanemura, T.; Soganci, I. M.; Oyama, T.; Ohyama, T.; Mino, S.; Williams, K. A.; Calabretta, N.; Dorren, H. J S; Nakano, Y.

2010-01-01

We demonstrate large-capacity high-resolution optical buffer, comprising 1×16 InP switch and ultra-compact delay lines based on thin-cladding highly nonlinear fibers. Silica-PLC-based pitch converter is employed to realize uniform coupling from all 16 switch ports simultaneously.

On the determination of the colour term in the P-L-C relation for cepheids

International Nuclear Information System (INIS)

Feast, M.W.; Balona, L.A.

1980-01-01

Recent speculations that the coefficient of the colour term in the P-L-C relation for cepheids may be considerably different from 2.7 are inconsistent with existing data on LMC cepheids. The colour coefficient recently derived is not significantly affected by taking into account an absorption induced correlation between the errors in 0 > and ( 0 > - 0 >). The computer simulation which shows the bias introduced in the colour coefficient when this is derived by an inappropriate method (simple least squares) is consistent with theory and in accord with actual results in the LMC. This gives added confidence in the unbiased nature of the results derived by a maximum likelihood method which takes into account errors in each variable. (author)

Department of Energy: monitoring and control of British Nuclear Fuels plc

International Nuclear Information System (INIS)

1989-01-01

British Nuclear Fuels plc (BNFL) was set up in 1971 to take over the nuclear fuel production and reprocessing activities of the United Kingdom Atomic Energy Authority with the Department of Energy (as majority shareholder) being responsible for the monitoring and control of BNFL's activities. BNFL's activities include the production of nuclear fuel, uranium enrichment, and the transportation and reprocessing of spent fuel. Its major capital investment includes the construction of the Thermal Oxide Reprocessing Plant (THORP) due for completion in 1992. This study examined the effectiveness of the Department's arrangements for monitoring and control and for safeguarding the Government's investment in the company, the arrangements for examining BNFL's capital investment programme and the extent to which the Department's main aims have been achieved. The examination was restricted to the financial performance. The National Audit Office found evidence to suggest that BNFL's financial performance has not kept pace with the general performance level of British Industry. Future success and performance will depend on the success of the THORP plant. (U.K.)

Realization of Intelligent Measurement and Control System for Limb Rehabilitation Based on PLC and Touch Screen

Science.gov (United States)

Liu, Xiangquan

According to the treatment needs of patients with limb movement disorder, on the basis of the limb rehabilitative training prototype, function of measure and control system are analyzed, design of system hardware and software is completed. The touch screen which is adopt as host computer and man-machine interaction window is responsible for sending commands and training information display; The PLC which is adopt as slave computer is responsible for receiving control command from touch screen, collecting the sensor data, regulating torque and speed of motor by analog output according to the different training mode, realizing ultimately active and passive training for limb rehabilitation therapy.

Development of Real Time Operating System for Safety Grade PLC (POSAFE-Q) for Nuclear Power Plants

International Nuclear Information System (INIS)

Son, Han Seong; Hwang, Sung Jae; Lee, Young Joon; Kim, Chang Hwoi; Lee, Dong Young

2006-01-01

POSAFE-Q is a newly developed programmable logic controller (PLC) in order to apply to digital safety system of nuclear power plants (NPP) according to Nuclear Power Plant safety requirements. POSAFE-Q hardware and software development process, including design, review, verification and validation, and configuration control and quality assurance, satisfies the requirements imposed by 10CFR50, Appendix B. This article introduces a real time operating system pCOS, which is the core of POSAFE-Q. Section 2 describes the structure of pCOS. Section 3 describes a few important features of pCOS, which are necessary to the application for the digital safety system of NPP

Development of Real Time Operating System for Safety Grade PLC (POSAFE-Q) for Nuclear Power Plants

Energy Technology Data Exchange (ETDEWEB)

Son, Han Seong [ENESYS, Taejon (Korea, Republic of); Hwang, Sung Jae [POSCON, Seoul (Korea, Republic of); Lee, Young Joon; Kim, Chang Hwoi; Lee, Dong Young [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

2006-07-01

POSAFE-Q is a newly developed programmable logic controller (PLC) in order to apply to digital safety system of nuclear power plants (NPP) according to Nuclear Power Plant safety requirements. POSAFE-Q hardware and software development process, including design, review, verification and validation, and configuration control and quality assurance, satisfies the requirements imposed by 10CFR50, Appendix B. This article introduces a real time operating system pCOS, which is the core of POSAFE-Q. Section 2 describes the structure of pCOS. Section 3 describes a few important features of pCOS, which are necessary to the application for the digital safety system of NPP.0.

Two-mode PLC-based mode multi/demultiplexer for mode and wavelength division multiplexed transmission.

Science.gov (United States)

Hanzawa, Nobutomo; Saitoh, Kuimasa; Sakamoto, Taiji; Matsui, Takashi; Tsujikawa, Kyozo; Koshiba, Masanori; Yamamoto, Fumihiko

2013-11-04

We proposed a PLC-based mode multi/demultiplexer (MUX/DEMUX) with an asymmetric parallel waveguide for mode division multiplexed (MDM) transmission. The mode MUX/DEMUX including a mode conversion function with an asymmetric parallel waveguide can be realized by matching the effective indices of the LP(01) and LP(11) modes of two waveguides. We report the design of a mode MUX/DEMUX that can support C-band WDM-MDM transmission. The fabricated mode MUX/DEMUX realized a low insertion loss of less than 1.3 dB and high a mode extinction ratio that exceeded 15 dB. We used the fabricated mode MUX/DEMUX to achieve a successful 2 mode x 4 wavelength x 10 Gbps transmission over a 9 km two-mode fiber with a penalty of less than 1 dB.

Design of Control System for Flexible Packaging Bags Palletizing Production Line Based on PLC

Science.gov (United States)

Zheng, Huiping; Chen, Lin; Zhao, Xiaoming; Liu, Zhanyang

Flexible packaging bags palletizing production line is to put the bags in the required area according to particular order and size, in order to finish handling, storage, loading and unloading, transportation and other logistics work of goods. Flexible packaging bags palletizing line is composed of turning bags mechanism, shaping mechanism, indexing mechanism, marshalling mechanism, pushing bags mechanism, pressing bags mechanism, laminating mechanism, elevator, tray warehouse, tray conveyor and loaded tray conveyor. Whether the whole production line can smoothly run depends on each of the above equipment and precision control among them. In this paper the technological process and the control logic of flexible packaging bags palletizing production line is introduced. Palletizing process of the production line realized automation by means of a control system based on programmable logic controller (PLC). It has the advantages of simple structure, reliable and easy maintenance etc.

Семинар «Система интегрированного поиска по электронным ресурсам EBSCO Discovery Service»: материалы семинара. Екатеринбург, 17 апреля 2012 г.

OpenAIRE

Соколов, А.; Вольф, Клаус

2012-01-01

17 апреля 2012 г. в Зональной научной библиотеке состоялся семинар «Система интегрированного поиска по электронным ресурсам: EBSCO Discovery Service». Программа семинара: 1. Интегрированный поиск EBSCO Discovery Service (Андрей Соколов, представитель компании EBSCO Publishing в России, Белоруссии и Молдове); 2. Технические аспекты работы EBSCO Discovery Service (Клаус Вольф, инженер-программист компании EBSCO Publishing (Германия)); 3. Демонстрация работы EDS в УрФУ....

Virtual Modular Redundancy of Processor Module in the PLC

International Nuclear Information System (INIS)

Lee, Kwang-Il; Hwang, SungJae; Yoon, DongHwa

2016-01-01

Dual Modular Redundancy (DMR) is mainly used to implement these safety control systems. DMR is conveyed when components of a system are duplicated, providing another component in case one should fault or fail. This feature has a high availability and large fault tolerant. It provides zero downtime that is required for nuclear power plants. So nuclear power plant has been commercialized by multiple redundant systems. The following paper, we propose a Virtual Modular Redundancy (VMR) rather than physical triple of the Programmable Logic Controller (PLC) processor module to ensure the reliability of the nuclear power plant control system. VMR implementation minimizes design changes to continue to use the commercially available redundant system. Also, the purpose of the VMR is to improve the efficiency and reliability in many ways, such as fault tolerant and fail-safe and cost. VMR guarantees a wide range of reliable fault recovery, fault tolerance, etc. It is prevented before it causes great damages due to the continuous failure of the two modules. The reliable communication speed is slow and also it has a small bandwidth. It is a great loss in the safety control system. However, VMR aims to avoid nuclear power plants that were suspended due to fail-safe. It is not for the purpose of commonly used. Application of VMR is actually expected to require a lot of research and trial and error until they adapt to the nuclear regulatory and standards

Virtual Modular Redundancy of Processor Module in the PLC

Energy Technology Data Exchange (ETDEWEB)

Lee, Kwang-Il; Hwang, SungJae; Yoon, DongHwa [SOOSAN ENS Co., Seoul (Korea, Republic of)

2016-10-15

Dual Modular Redundancy (DMR) is mainly used to implement these safety control systems. DMR is conveyed when components of a system are duplicated, providing another component in case one should fault or fail. This feature has a high availability and large fault tolerant. It provides zero downtime that is required for nuclear power plants. So nuclear power plant has been commercialized by multiple redundant systems. The following paper, we propose a Virtual Modular Redundancy (VMR) rather than physical triple of the Programmable Logic Controller (PLC) processor module to ensure the reliability of the nuclear power plant control system. VMR implementation minimizes design changes to continue to use the commercially available redundant system. Also, the purpose of the VMR is to improve the efficiency and reliability in many ways, such as fault tolerant and fail-safe and cost. VMR guarantees a wide range of reliable fault recovery, fault tolerance, etc. It is prevented before it causes great damages due to the continuous failure of the two modules. The reliable communication speed is slow and also it has a small bandwidth. It is a great loss in the safety control system. However, VMR aims to avoid nuclear power plants that were suspended due to fail-safe. It is not for the purpose of commonly used. Application of VMR is actually expected to require a lot of research and trial and error until they adapt to the nuclear regulatory and standards.

75 FR 801 - Airworthiness Directives; Rolls-Royce plc RB211-Trent 500, 700, and 800 Series Turbofan Engines

Science.gov (United States)

2010-01-06

...The FAA proposes to supersede an existing airworthiness directive (AD) for Rolls-Royce plc RB211-Trent 800 series turbofan engines. That AD currently requires replacing the fuel-to-oil heat exchanger (FOHE). This proposed AD would require replacing the FOHE on the RB211-Trent 500 and RB211-Trent 700 series turbofan engines in addition to the RB211-Trent 800 series turbofan engines. This proposed AD results from mandatory continuing airworthiness information (MCAI) issued by an aviation authority of another country to identify and correct an unsafe condition on an aviation product, and results from the risk of engine FOHE blockage. The MCAI describes the unsafe condition as:

Investigación y análisis de la tecnología PLC, desde la perspectiva del mercado ecuatoriano

OpenAIRE

Rivera Pacheco, Andrea Catalina

2008-01-01

Las Comunicaciones por Línea de Poder (PLC) aprovechan el tendido eléctrico existente como un medio de intercambio de señales para propósitos de comunicación. Consiste en acondicionar parte de las actuales infraestructuras eléctricas para que puedan transmitir señales regulares de baja frecuencia y otras por encima de la banda de 1 MHz, sin que se vea afectado el rendimiento eléctrico. Las señales de baja frecuencia (50 ó 60 Hz, según la red eléctrica) son las encargadas de la transmisión ...

Review of experience with plutonium exposure assessment methodologies at the nuclear fuel reprocessing site of British Nuclear Fuels plc

International Nuclear Information System (INIS)

Strong, R.

1988-01-01

British Nuclear Fuels plc and its predecessors have provided a complete range of nuclear fuel services to utilities in the UK and elsewhere for more than 30 years. Over 30,000 ton of Magnox and Oxide fuel have been reprocessed at Sellafield. During this time substantial experience has accumulated of methodologies for the assessment of exposure to actinides, mainly isotopes of plutonium. For most of the period monitoring of personnel included assessment of systemic uptake deduced from plutonium-in-urine results. The purpose of the paper is to present some conclusions of contemporary work in this area

Synthesis of FBD-based PLC design from NuSCR formal specification

International Nuclear Information System (INIS)

Yoo, Junbeom; Cha, Sungdeok; Kim, Chang Hwoi; Song, Duck Yong

2005-01-01

NuSCR is a formal specification language to document requirements for real-time embedded software with nuclear engineering applications in mind. Domain experts actively participated in selecting how to best represent various aspects. It uses tabular notations to specify required computations and automata to document state- or time-dependent behavior. As programmable logic controllers (PLCs) are widely used to implement real-time embedded software, synthesis of PLC code from a formal specification is desirable if transformation rules can be rigorously defined. In addition to improved productivity, results of safety analysis performed on requirements remain valid. In this paper, we demonstrate how NuSCR specification can be translated into semantically equivalent function block diagram (FBD) code. The process, except the initial phase where user provides information on missing or implicit details, is automated. Since executable code can be automatically generated using CASE tools from FBD, much of software development is automated. Proposed technique is currently being used in developing reactor protection system (RPS) for nuclear power plants in Korea, and experience to date has been positive. We demonstrate the proposed approach using the fixed set-point rising trip which is one of the most complex trip logics included in the RPS

Research and development of pyrochemical technologies for ADTT systems in NRI Rez plc

International Nuclear Information System (INIS)

Uhlir, J.

1999-01-01

The activities within the Czech national Partitioning and Transmutation program are oriented mainly toward the following areas: (i) ADS flowsheeting working out a proposal for a suitable technological flowsheet for treating spent fuel into a form fitted for transmutation reactor, including separation procedures before transmutation (Front-end) and separation processes after passage of fuel through the transmutor (Back-end). (ii) Technological research into the uranium component separation from spent fuel by the Fluoride Volatility Method semi-pilot-plant technological research based on the application of experience from spent fuel fluoride reprocessing. (iii) Laboratory research into electro-separation methods for the separation of fission products, plutonium and minor actinides from the fluoride melt. Selection of a suitable matrix composition of fluoride melts based on studies of their physicochemical properties and design of laboratory electro-separation facility. (iv) Development of materials and equipment for molten fluoride salts based ADS technologies, i.e. construction materials development, research into the corrosion resistance and development of equipment for molten fluoride salts technologies. The NRI Rez plc cooperates in this area with the SKODA Nuclear Machinery company

A robust and stable PLC based control system for 40kJ/25kV EMM system

Energy Technology Data Exchange (ETDEWEB)

Sharma, Vijay; Saroj, P.C.; Kulkarni, M.R.; Kumar, Satendra, E-mail: vijay9819420563@gmail.com [Accelerator and Pulse Power Division, Bhabha Atomic Research Centre, Mumbai (India)

2014-07-01

This paper describes the PLC based control system developed for a 40kJ/25kV Electro-magnetic machining (EMM) system. In EMM system large capacitor banks is charged with high voltage to store large energy and the banks is made to discharge into a coil within few milli-seconds using a triggered spark gaps. During discharge of the capacitor large surges and transients are generated in the system. The control system monitors/controls and interlocks all the units of the system for proper operation. The control system is the only subsystem which is electrically connected to all the low and high voltage subsystems. Care should be taken at the signal interfacing with the control system to protect the control system. (author)

A robust and stable PLC based control system for 40kJ/25kV EMM system

International Nuclear Information System (INIS)

Sharma, Vijay; Saroj, P.C.; Kulkarni, M.R.; Kumar, Satendra

2014-01-01

This paper describes the PLC based control system developed for a 40kJ/25kV Electro-magnetic machining (EMM) system. In EMM system large capacitor banks is charged with high voltage to store large energy and the banks is made to discharge into a coil within few milli-seconds using a triggered spark gaps. During discharge of the capacitor large surges and transients are generated in the system. The control system monitors/controls and interlocks all the units of the system for proper operation. The control system is the only subsystem which is electrically connected to all the low and high voltage subsystems. Care should be taken at the signal interfacing with the control system to protect the control system. (author)

Parathyroid Hormone Activates Phospholipase C (PLC)-Independent Protein Kinase C Signaling Pathway via Protein Kinase A (PKA)-Dependent Mechanism: A New Defined Signaling Route Would Induce Alternative Consideration to Previous Conceptions.

Science.gov (United States)

Tong, Guojun; Meng, Yue; Hao, Song; Hu, Shaoyu; He, Youhua; Yan, Wenjuan; Yang, Dehong

2017-04-20

BACKGROUND Parathyroid hormone (PTH) is an effective anti-osteoporosis agent, after binding to its receptor PTHR1, several signaling pathways, including cAMP/protein kinase A (PKA) and phospholipase C (PLC)/protein kinase C (PKC), are initiated through G proteins; with the cAMP/PKA pathway as the major pathway. Earlier studies have reported that PTHR1 might also activate PKC via a PLC-independent mechanism, but this pathway remains unclear. MATERIAL AND METHODS In HEK293 cells, cAMP accumulation was measured with ELISA and PKC was measured with fluorescence resonance energy transfer (FRET) analysis using CKAR plasmid. In MC3T3-E1 cells, real-time PCR was performed to examine gene expressions. Then assays for cell apoptosis, cell differentiation, alkaline phosphatase activity, and mineralization were performed. RESULTS The FRET analysis found that PTH(1-34), [G1,R19]PTH(1-34) (GR(1-34), and [G1,R19]PTH(1-28) (GR(1-28) were all activated by PKC. The PKC activation ability of GR(1-28) was blocked by cAMP inhibitor (Rp-cAMP) and rescued with the addition of active PKA-α and PKA-β. The PKC activation ability of GR(1-34) was partially inhibited by Rp-cAMP. In MC3T3-E1 cells, gene expressions of ALP, CITED1, NR4a2, and OSX that was regulated by GR(1-28) were significantly changed by the pan-PKC inhibitor Go6983. After pretreatment with Rp-cAMP, the gene expressions of ALP, CITED1, and OPG were differentially regulated by GR(1-28) or GR(1-34), and the difference was blunted by Go6983. PTH(1-34), GR(1-28), and GR(1-34) significantly decreased early apoptosis and augmented osteoblastic differentiation in accordance with the activities of PKA and PKC. CONCLUSIONS PLC-independent PKC activation induced by PTH could be divided into two potential mechanisms: one was PKA-dependent and associated with PTH(1-28); the other was PKA-independent and associated with PTH(29-34). We also found that PTH could activate PLC-independent PKC via PKA-dependent mechanisms.

Dopamine suppresses neuronal activity of Helisoma B5 neurons via a D2-like receptor, activating PLC and K channels.

Science.gov (United States)

Zhong, L R; Artinian, L; Rehder, V

2013-01-03

Dopamine (DA) plays fundamental roles as a neurotransmitter and neuromodulator in the central nervous system. How DA modulates the electrical excitability of individual neurons to elicit various behaviors is of great interest in many systems. The buccal ganglion of the freshwater pond snail Helisoma trivolvis contains the neuronal circuitry for feeding and DA is known to modulate the feeding motor program in Helisoma. The buccal neuron B5 participates in the control of gut contractile activity and is surrounded by dopaminergic processes, which are expected to release DA. In order to study whether DA modulates the electrical activity of individual B5 neurons, we performed experiments on physically isolated B5 neurons in culture and on B5 neurons within the buccal ganglion in situ. We report that DA application elicited a strong hyperpolarization in both conditions and turned the electrical activity from a spontaneously firing state to an electrically silent state. Using the cell culture system, we demonstrated that the strong hyperpolarization was inhibited by the D2 receptor antagonist sulpiride and the phospholipase C (PLC) inhibitor U73122, indicating that DA affected the membrane potential of B5 neurons through the activation of a D2-like receptor and PLC. Further studies revealed that the DA-induced hyperpolarization was inhibited by the K channel blockers 4-aminopyridine and tetraethylammonium, suggesting that K channels might serve as the ultimate target of DA signaling. Through its modulatory effect on the electrical activity of B5 neurons, the release of DA in vivo may contribute to a neuronal output that results in a variable feeding motor program. Copyright © 2012 IBRO. Published by Elsevier Ltd. All rights reserved.

Remediation of old environmental liabilities in the Nuclear Research Institute Rez plc

International Nuclear Information System (INIS)

Svoboda, Karel; Podlaha, Josef

2011-01-01

The Nuclear Research Institute Rez plc (NRI) after 55 years of activities in the nuclear field produced some environmental liabilities that shall be remedied. There are three areas of remediation: (1) decommissioning of old obsolete facilities (e.g. decay tanks, RAW treatment technology, special sewage system), (2) processing of RAW from operation and dismantling of nuclear facilities, and (3) elimination of spent fuel from research nuclear reactors operated by the NRI. The goal is to remedy the environmental liabilities and eliminate the potential negative impact on the environment. Remediation of the environmental liabilities started in 2003 and will be finished in 2014. The character of the environmental liabilities is very specific and requires special remediation procedures. Special technologies are being developed with assistance of external subcontractors. The NRI has gained many experiences in the field of RAW management and decommissioning of nuclear facilities and will use its facilities, experienced staff and all relevant data needed for the successful realization of the remediation. The most significant items of environmental liabilities are described in the paper together with information about the history, the current state, the progress, and the future activities in the field of remediation of environmental liabilities in the NRI. (author)

Operación de un PLC Mediante un PDA Vía ZIGBEE

Directory of Open Access Journals (Sweden)

Juan Zuluaga-Botero

2015-07-01

Full Text Available En este artículo se muestra la parte preliminar de interconexión de un módulo ZigBee con un Asistente Digital Personal (PDA a través de la red del Sistema de telefonía Móvil Global (GSM, para aplicación futura de comunicación con un Controlador Lógico Programable (PLC. Esta aplicación permite realizar monitoreo y control del sistema, de manera remota y con dispositivos móviles siendo el alcance del proyecto de investigación. Para este proyecto inicialmente se realizan comunicaciones que permiten empalmar las tecnologías de las redes de telefonía móvil celular con la red ZigBee, haciendo una interfaz transparente para el usuario, presentando dichos resultados en este artículo. Para esto se trabaja con tramas de datos básicas a través de la red de telefonía móvil celular, mediante mensajería corta recibida por el puerto serial del Zigbee remoto, utilizando microcontroladores para el control de los módulos que conforman esta parte del sistema de comunicación, estableciendo la interfaz con el dispositivo móvil o Smartphone vía Zigbee.

Pengaruh Problem-Based Learning terhadap Hasil Belajar ditinjau dari Motivasi Belajar PLC di SMK

Directory of Open Access Journals (Sweden)

Bekti Wulandari

2013-06-01

Full Text Available Penelitian ini bertujuan untuk mengetahui: (1 perbedaan hasil belajar siswa pada mata pelajaran pemrograman sistem kendali PLC antara siswa yang diajar dengan metode PBL dengan siswa yang diajar dengan metode demonstrasi, (2 pengaruh interaksi antara metode PBL dan metode demonstrasi dengan motivasi belajar terhadap hasil belajar siswa, (3 perbedaan hasil belajar siswa antara siswa yang diajar dengan metode PBL dengan yang diajar dengan metode demonstrasi ditinjau dari motivasi belajar. Penelitian ini merupakan penelitian eksperimen semu dengan desain faktorial yang dilakukan dengan memberikan perlakuan metode pembelajaran. Analisis data dalam penelitian ini  menggunakan uji-t dan ANAVA dengan program SPSS 16. Hasil penelitian menunjukkan bahwa: (1 terdapat perbedaan hasil belajar antara siswa yang diajar dengan metode PBL dengan yang diajar dengan metode demonstrasi, (2 tidak terdapat pengaruh interaksi antara metode PBL dan demonstrasi dengan motivasi belajar terhadap hasil belajar, (3 terdapat perbedaan hasil belajar antara siswa yang diajar dengan metode PBL dengan yang diajar dengan metode demonstrasi ditinjau dari motivasi tinggi dan rendah. Kata kunci: metode pembelajaran, Problem-Based Learning, motivasi belajar, hasil belajar

A Study on the Optimal Receiver Impedance for SNR Maximization in Broadband PLC

Directory of Open Access Journals (Sweden)

Massimo Antoniali

2013-01-01

Full Text Available We consider the design of the front-end receiver for broadband power line communications. We focus on the design of the input impedance that maximizes the signal-to-noise ratio (SNR at the receiver. We show that the amplitude, rather than the power, of the received signal is important for communication purposes. Furthermore, we show that the receiver impedance impacts the amplitude of the noise term. We focus on the background noise, and we propose a novel description of the noise experienced at the receiver port of a PLC network. We model the noise as the sum of four uncorrelated contributions, that is, the active, resistive, receiver, and coupled noise components. We study the optimal impedance design problem for real in-home grids that we assessed with experimental measurements. We describe the results of the measurement campaign, and we report the statistics of the optimal impedance. Hence, we study the best attainable performance when the optimal receiver impedance is deployed. We focus on the SNR and the maximum achievable rate, and we show that power matching is suboptimal with respect to the proposed impedance design approach.

Coordination of motor systems of the control bars of TRIGA Mark III reactor, through the use of a PLC; Coordinacion de los sistemas motrices de las barras de control del reactor Triga Mark III, mediante el uso de un PLC

Energy Technology Data Exchange (ETDEWEB)

Castro R, P. R.

2016-07-01

The use of programmable logic controllers (PLCs) has a wide field in the realization of automatic systems, since is sought that the form of control is easy for any user. In this work, the TRIGA Mark III reactor of Instituto Nacional de Investigaciones Nucleares (ININ) is intended to operate with a programming code in PLC for the automation of the control rods, having uniform wear according to the power required by the user. On the other hand, is proposed to develop an HMI graphical interface for communication via Ethernet, allowing supervision during the reactor operation process and greater protection of operators during reactor startup. The accuracy of the new actuators, as well as their durability, will allow a good performance of the reactor for many years to come. (Author)

Estudio para la automatización de la lectura, corte y reconexión de la energía eléctrica mediante tecnología PLC (Power Line Communication). Caso de análisis : Empresa Eléctrica Regional CentroSur C.A.

OpenAIRE

Quizhpi Montero, Diego Armando; Ruiz Orellana, José Luis

2007-01-01

La finalidad de esta tesis es entregar una mayor información sobre la tecnología PLC, como sus bases teóricas y de cómo aprovecha el tendido de red eléctrica para la lectura, corte y reconexión de los contadores de energía eléctrica mediante la utilización de equipos adecuados. La tecnología PLC también permite ofrecer una diversidad de servicios de telecomunicaciones, tales como: voz, video y datos, el cual se lo conoce como triple-play. Al momento de transmitir datos por el tendido el...

Molecular characterization of severin from Clonorchis sinensis excretory/secretory products and its potential anti-apoptotic role in hepatocarcinoma PLC cells.

Directory of Open Access Journals (Sweden)

Xueqing Chen

Full Text Available BACKGROUND: Clonorchiasis, caused by the infection of Clonorchis sinensis (C. sinensis, is a kind of neglected tropical disease, but it is highly related to cholangiocarcinoma and hepatocellular carcinoma (HCC. It has been well known that the excretory/secretory products of C. sinensis (CsESPs play key roles in clonorchiasis associated carcinoma. From genome and transcriptome of C. sinensis, we identified one component of CsESPs, severin (Csseverin, which had three putative gelsolin domains. Its homologues are supposed to play a vital role in apoptosis resistance of tumour cell. METHODOLOGY/PRINCIPAL FINDINGS: There was significant similarity in tertiary structures between human gelsolin and Csseverin by bioinformatics analysis. We identified that Csseverin expressed at life stage of adult worm, metacercaria and egg by the method of quantitative real-time PCR and western blotting. Csseverin distributed in vitellarium and intrauterine eggs of adult worm and tegument of metacercaria by immunofluorence assay. We obtained recombinant Csseverin (rCsseverin and confirmed that rCsseverin could bind with calciumion in circular dichroism spectrum analysis. It was demonstrated that rCsseverin was of the capability of actin binding by gel overlay assay and immunocytochemistry. Both Annexin V/PI assay and mitochondrial membrane potential assay of human hepatocarcinoma cell line PLC showed apoptosis resistance after incubation with different concentrations of rCsseverin. Morphological analysis, apoptosis-associated changes of mitochondrial membrane potential and Annexin V/PI apoptosis assay showed that co-incubation of PLC cells with rCsseverin in vitro led to an inhibition of apoptosis induced by serum-starved for 24 h. CONCLUSIONS/SIGNIFICANCE: Collectively, the molecular properties of Csseverin, a molecule of CsESPs, were characterized in our study. rCsseverin could cause obvious apoptotic inhibition in human HCC cell line. Csseverin might exacerbate the

Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells

Science.gov (United States)

He, Lei; Wang, Xiaoyun; Liang, Pei; Chen, Wenjun; Bian, Meng; Ren, Mengyu; Lin, Jinsi; Liang, Chi; Xu, Jin; Wu, Zhongdao; Li, Xuerong; Huang, Yan; Yu, Xinbing

2013-01-01

Background Clonorchiasis, caused by the infection of Clonorchis sinensis (C. sinensis), is a kind of neglected tropical disease, but it is highly related to cholangiocarcinoma and hepatocellular carcinoma (HCC). It has been well known that the excretory/secretory products of C. sinensis (CsESPs) play key roles in clonorchiasis associated carcinoma. From genome and transcriptome of C. sinensis, we identified one component of CsESPs, severin (Csseverin), which had three putative gelsolin domains. Its homologues are supposed to play a vital role in apoptosis resistance of tumour cell. Methodology/Principal Findings There was significant similarity in tertiary structures between human gelsolin and Csseverin by bioinformatics analysis. We identified that Csseverin expressed at life stage of adult worm, metacercaria and egg by the method of quantitative real-time PCR and western blotting. Csseverin distributed in vitellarium and intrauterine eggs of adult worm and tegument of metacercaria by immunofluorence assay. We obtained recombinant Csseverin (rCsseverin) and confirmed that rCsseverin could bind with calciumion in circular dichroism spectrum analysis. It was demonstrated that rCsseverin was of the capability of actin binding by gel overlay assay and immunocytochemistry. Both Annexin V/PI assay and mitochondrial membrane potential assay of human hepatocarcinoma cell line PLC showed apoptosis resistance after incubation with different concentrations of rCsseverin. Morphological analysis, apoptosis-associated changes of mitochondrial membrane potential and Annexin V/PI apoptosis assay showed that co-incubation of PLC cells with rCsseverin in vitro led to an inhibition of apoptosis induced by serum-starved for 24 h. Conclusions/Significance Collectively, the molecular properties of Csseverin, a molecule of CsESPs, were characterized in our study. rCsseverin could cause obvious apoptotic inhibition in human HCC cell line. Csseverin might exacerbate the process of HCC

Inhibitory effect of genistein on PLC/PRF5 hepatocellular carcinoma cell line

Directory of Open Access Journals (Sweden)

Mehdi Nikbakht Dastjerdi

2015-01-01

Full Text Available Background: Natural compounds including flavonoids like genistein (GE are able to inhibit cell proliferation and induce apoptosis. GE is the main representative of these groups. GE inhibits carcinogenic tumors such as colon, stomach, lung, and pancreas tumors. The aim of the present study was to analyze the apoptotic effect of GE in the hepatocellular carcinoma (HCC PLC/PRF5 cell line. Methods: Cells were treated with various doses of GE (1, 5, 10, 25, 50, 75, and 100 μM/L at different times (24, 48, and 72 h and the MTT assay was commonly used. Furthermore, cells were treated with single dose of GE (25 μM at different times and flow cytometry was performed. Results: GE inhibited the growth of liver cancer cells significantly with a time- and dose-dependent manner. The percentage of living cells in GE treatment groups with a concentration of 25 μM at different times were 53, 48 and 47%, respectively (P < 0.001. Result of flow cytometry demonstrated that GE at a 25 μM concentration induces apoptosis significantly in a time-dependent manner. The percentage of apoptotic cells at different times were 44, 56, and 60%, respectively (P < 0.001. Conclusions: GE can significantly inhibit the growth of HCC cells and plays a significant role in apoptosis of this cell line.

Coordination of motor systems of the control bars of TRIGA Mark III reactor, through the use of a PLC

International Nuclear Information System (INIS)

Castro R, P. R.

2016-01-01

The use of programmable logic controllers (PLCs) has a wide field in the realization of automatic systems, since is sought that the form of control is easy for any user. In this work, the TRIGA Mark III reactor of Instituto Nacional de Investigaciones Nucleares (ININ) is intended to operate with a programming code in PLC for the automation of the control rods, having uniform wear according to the power required by the user. On the other hand, is proposed to develop an HMI graphical interface for communication via Ethernet, allowing supervision during the reactor operation process and greater protection of operators during reactor startup. The accuracy of the new actuators, as well as their durability, will allow a good performance of the reactor for many years to come. (Author)

TRPV1 and PLC Participate in Histamine H4 Receptor-Induced Itch.

Science.gov (United States)

Jian, Tunyu; Yang, Niuniu; Yang, Yan; Zhu, Chan; Yuan, Xiaolin; Yu, Guang; Wang, Changming; Wang, Zhongli; Shi, Hao; Tang, Min; He, Qian; Lan, Lei; Wu, Guanyi; Tang, Zongxiang

2016-01-01

Histamine H4 receptor has been confirmed to play a role in evoking peripheral pruritus. However, the ionic and intracellular signaling mechanism of activation of H4 receptor on the dorsal root ganglion (DRG) neurons is still unknown. By using cell culture and calcium imaging, we studied the underlying mechanism of activation of H4 receptor on the DRG neuron. Immepip dihydrobromide (immepip)-a histamine H4 receptor special agonist under cutaneous injection-obviously induced itch behavior of mice. Immepip-induced scratching behavior could be blocked by TRPV1 antagonist AMG9810 and PLC pathway inhibitor U73122. Application of immepip (8.3-50 μM) could also induce a dose-dependent increase in intracellular Ca(2+) ([Ca(2+)]i) of DRG neurons. We found that 77.8% of the immepip-sensitized DRG neurons respond to the TRPV1 selective agonist capsaicin. U73122 could inhibit immepip-induced Ca(2+) responses. In addition, immepip-induced [Ca(2+)]i increase could be blocked by ruthenium red, capsazepine, and AMG9810; however it could not be blocked by TRPA1 antagonist HC-030031. These results indicate that TRPV1 but not TRPA1 is the important ion channel to induce the DRG neurons' responses in the downstream signaling pathway of histamine H4 receptor and suggest that TRPV1 may be involved in the mechanism of histamine-induced itch response by H4 receptor activation.

TRPV1 and PLC Participate in Histamine H4 Receptor-Induced Itch

Directory of Open Access Journals (Sweden)

Tunyu Jian

2016-01-01

Full Text Available Histamine H4 receptor has been confirmed to play a role in evoking peripheral pruritus. However, the ionic and intracellular signaling mechanism of activation of H4 receptor on the dorsal root ganglion (DRG neurons is still unknown. By using cell culture and calcium imaging, we studied the underlying mechanism of activation of H4 receptor on the DRG neuron. Immepip dihydrobromide (immepip—a histamine H4 receptor special agonist under cutaneous injection—obviously induced itch behavior of mice. Immepip-induced scratching behavior could be blocked by TRPV1 antagonist AMG9810 and PLC pathway inhibitor U73122. Application of immepip (8.3–50 μM could also induce a dose-dependent increase in intracellular Ca2+ (Ca2+i of DRG neurons. We found that 77.8% of the immepip-sensitized DRG neurons respond to the TRPV1 selective agonist capsaicin. U73122 could inhibit immepip-induced Ca2+ responses. In addition, immepip-induced Ca2+i increase could be blocked by ruthenium red, capsazepine, and AMG9810; however it could not be blocked by TRPA1 antagonist HC-030031. These results indicate that TRPV1 but not TRPA1 is the important ion channel to induce the DRG neurons’ responses in the downstream signaling pathway of histamine H4 receptor and suggest that TRPV1 may be involved in the mechanism of histamine-induced itch response by H4 receptor activation.

The Influence of Marketing Intelligence on Business Competitive Advantage (A Study of Diamond Bank Plc

Directory of Open Access Journals (Sweden)

Ladipo Patric Kunle Ade

2017-03-01

Full Text Available This study investigates the influence of Marketing Intelligence on Business Competitive Advantage: A study of Diamond Bank Plc, Nigeria. Five objectives were identified and translated into five research questions which aptly answered by subjecting them to a test of hypotheses. A Descriptive research was used to survey 292 members of the staff of Diamond Bank in Lagos, Central Regional branches and head office. Out of which 285 responses were obtained, while 6 responses were destroyed because they were not properly filled. Also, data was collected through a self-administered questionnaire, as Pearson correlation, T-test and Regression were used to test the hypotheses statements. However, the result of the findings revealed that marketing intelligence sub-constructs such as internal records, competitor’s sales data, marketplace opportunity, competitors’ threats and competitors’ risks have significant and positive influence on business competitive advantage. Thus, it can be concluded that marketing intelligence as sensitive information has enabled the bank to successfully acquire more profit, expand the branch network all over the country, perform better than its rivals in the market and increase its business competitive advantage.

Women in the Military: A Selected Bibliography

Science.gov (United States)

2009-08-01

Military: Where They Stand, 5th ed. Washington, DC: WREI [Women’s Research and Education Institute], April 2005. 37pp. (UB418 .W65M14 2005) Oliver...34Women in War: Operational Issues of Menstruation and Unintended Pregnancy." Military Medicine 172 (January 2007): 9-16. Ingenta Dove, Mary Baker, and...Publishing Group, 2004. 112pp. (D790 .D66 2004) Fort Des Moines Museum & Education Center. Fort Des Moines Museum & Education Center Home Page. http

PLC-dependent intracellular Ca2+ release was associated with C6-ceramide-induced inhibition of Na+ current in rat granule cells.

Science.gov (United States)

Liu, Zheng; Fei, Xiao-Wei; Fang, Yan-Jia; Shi, Wen-Jie; Zhang, Yu-Qiu; Mei, Yan-Ai

2008-09-01

In this report, the effects of C(6)-ceramide on the voltage-gated inward Na(+) currents (I(Na)), two types of main K(+) current [outward rectifier delayed K(+) current (I(K)) and outward transient K(+) current (I(A))], and cell death in cultured rat cerebellar granule cells were investigated. At concentrations of 0.01-100 microM, ceramide produced a dose-dependent and reversible inhibition of I(Na) without alteration of the steady-state activation and inactivation properties. Treatment with C(2)-ceramide caused a similar inhibitory effect on I(Na). However, dihydro-C(6)-ceramide failed to modulate I(Na). The effect of C(6)-ceramide on I(Na) was abolished by intracellular infusion of the Ca(2+)-chelating agent, 1,2-bis (2-aminophenoxy) ethane-N, N, N9, N9-tetraacetic acid, but was mimicked by application of caffeine. Blocking the release of Ca(2+) from the sarcoplasmic reticulum with ryanodine receptor blocker induced a gradual increase in I(Na) amplitude and eliminated the effect of ceramide on I(Na). In contrast, the blocker of the inositol 1,4,5-trisphosphate-sensitive Ca(2+) receptor did not affect the action of C(6)-ceramide. Intracellular application of GTPgammaS also induced a gradual decrease in I(Na) amplitude, while GDPbetaS eliminated the effect of C(6)-ceramide on I(Na). Furthermore, the C(6)-ceramide effect on I(Na) was abolished after application of the phospholipase C (PLC) blockers and was greatly reduced by the calmodulin inhibitors. Fluorescence staining showed that C(6)-ceramide decreased cell viability and blocking I(Na) by tetrodotoxin did not mimic the effect of C(6)-ceramide, and inhibiting intracellular Ca(2+) release by dantrolene could not decrease the C(6)-ceramide-induced cell death. We therefore suggest that increased PLC-dependent Ca(2+) release through the ryanodine-sensitive Ca(2+) receptor may be responsible for the C(6)-ceramide-induced inhibition of I(Na), which does not seem to be associated with C(6)-ceramide-induced granule

Système de PLC pour la supervision de la distribution basse tension des expériences ALICE et LHCb

CERN Document Server

Burdet, G

2004-01-01

Le groupe TS/EL étudie et réalise la distribution électrique basse tension pour alimenter les racks contenant l'électronique associée aux détecteurs dans les zones expérimentales du LHC. La distribution basse tension utilisera des tableaux récupérés des expériences du LEP pour ALICE et LHCb et un système basé sur des gaines CANALIS pour ATLAS et CMS. L'ensemble de la distribution électrique basse tension sera surveillée et contrôlée par l'intermédiaire de systèmes à base de PLC. Ce papier décrit l'infrastructure de supervision proposée pour ALICE et LHCb et son intégration au CERN Electrical Network Supervisor (ENS) et au Detector Control System (DCS).

A Technique of Software Safety Analysis in the Design Phase for PLC Based Safety-Critical Systems

International Nuclear Information System (INIS)

Koo, Seo-Ryong; Kim, Chang-Hwoi

2017-01-01

The purpose of safety analysis, which is a method of identifying portions of a system that have the potential for unacceptable hazards, is firstly to encourage design changes that will reduce or eliminate hazards and, secondly, to conduct special analyses and tests that can provide increased confidence in especially vulnerable portions of the system. For the design and implementation phase of the PLC based systems, we proposed a technique for software design specification and analysis, and this technique enables us to generate software design specifications (SDSs) in nuclear fields. For the safety analysis in the design phase, we used architecture design blocks of NuFDS to represent the architecture of the software. On the basis of the architecture design specification, we can directly generate the fault tree and then use the fault tree for qualitative analysis. Therefore, we proposed a technique of fault tree synthesis, along with a universal fault tree template for the architecture modules of nuclear software. Through our proposed fault tree synthesis in this work, users can use the architecture specification of the NuFDS approach to intuitively compose fault trees that help analyze the safety design features of software.

Purification of HBsAg produced by the human hepatoma cell line PLC/PRE/5 by affinity chromatography using monoclonal antibodies and application for ELISA diagnostic.

Science.gov (United States)

Merten, O W; Reiter, S; Scheirer, W; Katinger, H

1983-01-01

The human cell line PLC/PRF/5 (5) was used for the production of hepatitis B surface antigen subtype ad (HBsAg ad) and purified by affinity chromatography (AC) with monoclonal antibodies (mAb). mAb to HBsAg from mouse ascites have been purified by Protein A - AC prior coupling to AH-Sepharose 4B (Pharmacia). The combined procedure of ammonium-sulphate-precipitation of HBsAg from culture supernatants and immunosorbent-AC leads to approx. 700-fold purification. ELISA results using the mAb and the HBsAg for diagnostics of human serum, positive for anti-HBsAg-antibodies correlate with the RIA (AUSAB, Abbott).

MathWorks Simulink and C++ integration with the new VLT PLC-based standard development platform for instrument control systems

Science.gov (United States)

Kiekebusch, Mario J.; Di Lieto, Nicola; Sandrock, Stefan; Popovic, Dan; Chiozzi, Gianluca

2014-07-01

ESO is in the process of implementing a new development platform, based on PLCs, for upcoming VLT control systems (new instruments and refurbishing of existing systems to manage obsolescence issues). In this context, we have evaluated the integration and reuse of existing C++ libraries and Simulink models into the real-time environment of BECKHOFF Embedded PCs using the capabilities of the latest version of TwinCAT software and MathWorks Embedded Coder. While doing so the aim was to minimize the impact of the new platform by adopting fully tested solutions implemented in C++. This allows us to reuse the in house expertise, as well as extending the normal capabilities of the traditional PLC programming environments. We present the progress of this work and its application in two concrete cases: 1) field rotation compensation for instrument tracking devices like derotators, 2) the ESO standard axis controller (ESTAC), a generic model-based controller implemented in Simulink and used for the control of telescope main axes.

Department of Energy: monitoring and control of British Nuclear Fuels plc. Report by the Comptroller and Auditor General

Energy Technology Data Exchange (ETDEWEB)

1989-01-01

British Nuclear Fuels plc (BNFL) was set up in 1971 to take over the nuclear fuel production and reprocessing activities of the United Kingdom Atomic Energy Authority with the Department of Energy (as majority shareholder) being responsible for the monitoring and control of BNFL's activities. BNFL's activities include the production of nuclear fuel, uranium enrichment, and the transportation and reprocessing of spent fuel. Its major capital investment includes the construction of the Thermal Oxide Reprocessing Plant (THORP) due for completion in 1992. This study examined the effectiveness of the Department's arrangements for monitoring and control and for safeguarding the Government's investment in the company, the arrangements for examining BNFL's capital investment programme and the extent to which the Department's main aims have been achieved. The examination was restricted to the financial performance. The National Audit Office found evidence to suggest that BNFL's financial performance has not kept pace with the general performance level of British Industry. Future success and performance will depend on the success of the THORP plant. (U.K.).

The effect of feeding a commercial essential oil product on Clostridium perfringens numbers in the intestine of broiler chickens measured by real-time PCR targeting the α-toxin-encoding gene (plc)

DEFF Research Database (Denmark)

Abildgaard, Lone; Højberg, Ole; Schramm, Andreas

2010-01-01

Proliferation of Clostridium perfringens type A in the broiler intestinal tract is related to poor growth and litter quality, and can under certain conditions lead to the development of necrotic enteritis (NE), a severe gastrointestinal disease in broilers. The aim of the present study was to inv...... quantification of C. perfringens type A in broilers, a real-time PCR assay, targeting the α-toxin-encoding plc gene, was developed for use in ileal and caecal samples and was shown to be a fast and reliable alternative to conventional plate counting....

Automation of water supply and recirculation-filtration of water at a swimming pool using Zelio PLC

Science.gov (United States)

Diniş, C. M.; Popa, G. N.; Iagăr, A.

2018-01-01

The paper proposes the use of the Zelio PLC for the automation of the water supply and recirculation-filtration system of a swimming pool. To do this, the Zelio SR3B261BD - 24V DC with 10 digital inputs (24V DC) and 10 digital outputs (relay contacts) was used. The proposed application makes the control of the water supply pumps and the water recirculation-filtration from a swimming pool. The recirculation-filtration systems for pools and swimming pools are designed to ensure water cleaning and recirculation to achieve optimum quality and lasting service life. The water filtration process is one of the important steps in water treatment in polls and swimming pools. It consists in recirculation of the entire volume of water and begins by absorbing the water in the pool by means of a pump followed by the passing of water through the filter, disinfectant and pH dosing, and reintroducing the water back into the pool or swimming pool through the discharge holes. Filters must to work 24 hours a day to remove pollutants from pools or swimming pools users. Filtration removes suspension particles with different origins. All newly built pools and swimming pools must be fitted with water recirculation systems, and existing ones will be equipped with water recirculation and water treatment systems.

System of institutional radioactive waste management in the Nuclear Research Institute Rez plc

International Nuclear Information System (INIS)

Podlaha, J.; Burian, P.

2005-01-01

The Nuclear Research Institute Rez plc (NRI) is a leading institution in the area of nuclear Research and Development in the Czech Republic. The NRI has had a dominant position in the nuclear programme since it was established in 1955 as a state-owned research organization and it has developed to its current status. In December 1992 the NRI has been transformed into a joint-stock company. The NRI's activity encompasses nuclear physics, chemistry, nuclear power, experiments at the research reactor and many other topics. Main issues addressed in the NRI in the past decades were concentrated on research, development and services provided to the nuclear power plants operating WWER reactors, development of chemical technologies for fuel cycle and irradiation services to research and development in the industrial sector, agriculture, food processing and medicine. At present the research activities are mainly targeted to assist the State Office for Nuclear Safety -the nuclear safety regulating body, power plant operator and nuclear facilities contractors. Significant attention is also paid to the use of nuclear technology outside the nuclear power sector, providing a wide range of services to industry , medicine and the preparation of radiopharmaceuticals. NRI operates two research nuclear reactors and another facilities such as a hot cell facility , research laboratories, technology for radioactive waste (RAW) management, 60 Co irradiators, an electron accelerator, etc. In this paper the Centre of RAW management, system of RAW management, facilities for RAW management as well as decontamination and decommissioning activities of the NRI are presented. The NRI provides complex services in the area of RAW management and has gained many experience and full qualification not only in this area but also in the area of decontamination and decommissioning and spent fuel management. The NRI guarantees safe RAW and spent fuel management. (authors)

New Concept of PLC Modems: Multi-Carrier System for Frequency Selective Slow-Fading Channels Based on Layered SCCC Turbocodes

Directory of Open Access Journals (Sweden)

J. Zavrtalek

2015-09-01

Full Text Available The article introduces a novel concept of a PLC modem as a complement to the existing G3 and PRIME standards for communications using medium- or high-voltage overhead or cable lines. The proposed concept is based on the fact that the levels of impulse noise and frequency selectivity are lower on high-voltage lines than on low-voltage ones. Also, the demands for “cost-effective” circuitry design are not so crucial as in the case of modems for low-voltage level. In contract to these positive conditions, however, there is the need to overcome much longer distances and to take into account low SNR on the receiving side. With respect to the listed reasons, our concept makes use of MCM, instead of OFDM. The assumption of low SNR is compensated through the use of an efficient channel coding based on a serially concatenated turbo code. In addition, MCM offers lower latency and PAPR compared to OFDM. Therefore, when using MCM, it is possible to excite the line with higher power. The proposed concept has been verified during experimental transmission of testing data over a real, 5 km long, 22kV overhead line.

Fundamentación de la pertinencia y factibilidad de la aplicación domótica mediante la tecnología Power Line Communications PLC para redes domésticas en el Ecuador.

OpenAIRE

Suárez Ramos, Diego Fernando

2015-01-01

En el presente trabajo se fundamenta la pertinencia y factibilidad de la aplicación domótica mediante la tecnología Power Line Communications PLC, para redes domésticas en el Ecuador. Demostrando que es posible utilizar esta tecnología en los hogares ecuatorianos como una alternativa en los sistemas domóticos existentes. Se presenta como ejemplo diseño para el control de un sistema de iluminación, integrándolo junto con un panel domótico en un domicilio la red eléctric existente.

Distribution of Insertion- and Deletion-Associated Genetic Polymorphisms among Four Mycobacterium tuberculosis Phospholipase C Genes and Associations with Extrathoracic Tuberculosis: a Population-Based Study

OpenAIRE

Kong, Y.; Cave, M. D.; Yang, D.; Zhang, L.; Marrs, C. F.; Foxman, B.; Bates, J. H.; Wilson, F.; Mukasa, L. N.; Yang, Z. H.

2005-01-01

The Mycobacterium tuberculosis genome contains four phospholipase C (PLC)-encoding genes, designated plcA, plcB, plcC, and plcD, respectively. Each of the four genes contributes to the overall PLC activity of M. tuberculosis. PLC is hypothesized to contribute to M. tuberculosis virulence. Infection of M. tuberculosis strains carrying a truncated plcD gene is associated with the occurrence of extrathoracic tuberculosis. However, whether the other three plc genes are also associated with extrat...

DISAIN SISTEM KENDALI MESIN AIR LEAK TEST MENGGUNAKAN SISTEM KENDALI PLC OMRON CJ2M DI HVAC (HEATING, VENTILATING, AND AIR CONDITIONING LINE 6

Directory of Open Access Journals (Sweden)

Syahril Ardi

2015-03-01

Full Text Available Pada proses produksi pembuatan komponen HVAC (Heating, Ventilating, and Air Conditioning dari perusahaan manufaktur di Indonesia, memerlukan proses pengecekan kebocoran pada bagian HVAC. Proses pengecekan ini dilakukan untuk memastikan tidak ada komponen HVAC yang bocor sebelum dikirim ke pihak pelanggan. Penelitian ini dilakukan untuk membuat system dan alat air leak test. Mesin air leak test ini menggunakan prinsip kerja differential pressure air leak test, yaitu metode yang membandingkan antara tekanan udara yang diberikan ke produk dan master produk. Pada penelitian ini, kami membuat disain mesin air leak test menggunakan sistem kendali berupa air leak tester, PLC, dan HMI. Berdasarkan kondisi dengan kapasitas produksi yang meningkat karena bertambahnya permintaan dari customer, dapat ditanggulangi dengan adanya share loading produksi dari HVAC line 4 ke line baru, yaitu HVAC line 6. Hasil yang didapat dari pengujian deteksi kebocoran produk,didapat nilai parameter kebocoran produk sebesar 2.23 ml/min.

Prognostic significance of pleural lavage cytology after thoracotomy and before closure of the chest in lung cancer.

Science.gov (United States)

Taniguchi, Yuji; Nakamura, Hiroshige; Miwa, Ken; Adachi, Yoshin; Fujioka, Shinji; Haruki, Tomohiro; Horie, Yasushi

2009-07-01

Some reports have described pleural lavage cytology (PLC) to be a prognostic factor for non-small cell lung cancer (NSCLC) patients. However, there have only been a few reports describing the findings both immediately after thoracotomy (PLC after thoracotomy) and before the closure of the chest (PLC before closure). From April 2002 to April 2008, both PLC after thoracotomy and PLC before closure were performed in 296 consecutive patients who underwent resections for NSCLC. PLC after thoracotomy was positive in 14 patients. The survival rate in the PLC after thoracotomy positive cases was significantly poorer than in PLC after thoracotomy negative cases (P=0.047). In contrast, there were 26 PLC before closure positive cases. The survival rate in the PLC before closure positive cases was significantly poorer than in the PLC before closure negative cases (PPLC after thoracotomy is not an independent prognostic factor in our study. However, PLC before closure was an independent prognostic factor based on multivariate analyses. We conclude that PLC before closure was found to be a better prognostic factor than PLC after thoracotomy for NSCLC patients.

Vietnam Land Policy - Adjusting to Globalization

Science.gov (United States)

2013-11-01

the Late- Socialist Period," Pacific Affairs: vol 84, no. 3, (2011): 439-440, accessed 30 September 2013, EBSCO Discovery Service . 37 U.S...Society,” Contemporary Southeast Asia vol 31, no. 1 (2009): 21, accessed 15 October 2013, EBSCO Discovery Service . 81 Legal Information... EBSCO Discovery Service . Fforde, Adam. "Vietnam in 2012: The End of the Party." Asian Survey 53, no. 1 (2013): 101-108. Accessed 30

Distribution of phospholipase C isozymes in various rat tissues and cultured cells

International Nuclear Information System (INIS)

Suh, P.G.; Ryu, S.H.; Choi, W.C.; Lee, K.Y.; Rhee, S.G.

1987-01-01

Monoclonal antibodies prepared against PLC-I or PLC-II enzyme did not cross-react with the other. Using a pair of antibodies which recognizes 2 different antigenic sites on the same molecule, radioimmunoassays were developed for the quantitation of PLC-I and PLC-II in homogenates of various tissues and cultured cells, prepared by homogenization in a 2 M KCl buffer. The contents of PLC enzymes were measured in 19 rat tissues, in human platelets and in 17 cultured cells. Results indicate that the concentration of PLC-I and PLC-II is very high in brain, PLC-I is localized mainly in brain and partly in seminal vesicles, PLC-II is found in most tissues and cells. PLC-I is highly localized even in brain: 5 different neuroblastoma did not contain PLC-I while 2 glioma and 1 astrocytoma contained significant amounts

Membrane associated phospholipase C from bovine brain

International Nuclear Information System (INIS)

Lee, K.; Ryu, S.H.; Suh, P.; Choi, W.C.; Rhee, S.G.

1987-01-01

Cytosolic fractions of bovine brain contain 2 immunologically distinct phosphoinositide-specific phospholipase (PLC), PLC-I and PLC-II, whose MW are 150,000 and 145,000 respectively, under a denaturing condition. Monoclonal antibodies were derived against each form and specific radioimmunoassays were developed. Distribution of PLC-I and PLC-II in cytosolic and particulate fractions was measured using the radioimmunoassay. More than 90% of PLC-II was found in the cytosolic fraction, while the anti-PLC-I antibody cross-reacting protein was distributed nearly equally between the soluble fraction and the 2 M KCl extract of particulate fraction. The PLC enzyme in the particulate fraction was purified to homogeneity, yielding 2 proteins of 140 KDa and 150 KDa when analyzed on SDS-PAGE. Neither of the 2 enzymes cross-reacted with anti-PLC-II antibodies, but both could be immunoblotted by all 4 different anti-PLC-I antibodies. This suggests that the 140 KDa PLC was derived from the 150 KDa form. The 150 Kda form from particulate fraction was indistinguishable from the cytosolic PLC-I when their mixture was analyzed on SDS-PAGE. In addition, the elution profile of tryptic peptides derived from the 150 KDa particulate form was identical to that of cytosolic PLC-I. This result indicates that PLC-I is reversibly associated to membranes

Prediction of posterior ligamentous complex injury in thoracolumbar fractures using non-MRI imaging techniques.

Science.gov (United States)

Rajasekaran, Shanmuganathan; Maheswaran, Anupama; Aiyer, Siddharth N; Kanna, Rishi; Dumpa, Srikanth Reddy; Shetty, Ajoy Prasad

2016-06-01

We aimed to formulate a radiological index based on plain radiographs and computer tomography (CT) to reliably detect posterior ligamentous complex (PLC) injury without need for MRI. Sixty out of 148 consecutive thoracolumbar fractures with doubtful PLC were assessed with MRI, CT and radiographs. PLC injury was assessed with the following radiological parameters: superior-inferior end plate angle (SIEA), vertebral body height (BH), local kyphosis (LK), inter-spinous distance (ISD) and inter-pedicular distance (IPD) and correlated with MRI findings of PLC injury. Statistical analysis was performed to identify the predictive values for the parameters to identify PLC damage. MRI identified PLC injury in 25/60 cases. The ISD and LK were found to be significant predictors of PLC injury. On radiographs the mean LK with PLC damage was 25.86° compared to 21.02° with an intact PLC (p = 0.006). The ISD difference was 6.70 mm in cases with PLC damage compared to 2.86 mm with an intact PLC (p = 0.011). In CT images, the mean LK with PLC damage was 22.96° compared to 18.44° with an intact PLC ( p = 0.019). The ISD difference was 3.10 mm with PLC damage compared to 1.62 mm without PLC damage (p = 0.005). On plain radiographs the presence of LK greater than 20 °(CI 64-95) and ISD difference greater than 2 mm (CI 70-97) can predict PLC injury. These guidelines may be utilised in the emergency room especially when the associated cost, availability and time delay in performing MRI are a concern.

Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells.

Science.gov (United States)

Béziau, Delphine M; Toussaint, Fanny; Blanchette, Alexandre; Dayeh, Nour R; Charbel, Chimène; Tardif, Jean-Claude; Dupuis, Jocelyn; Ledoux, Jonathan

2015-01-01

Phospholipase C (PLC) comprises a superfamily of enzymes that play a key role in a wide array of intracellular signalling pathways, including protein kinase C and intracellular calcium. Thirteen different mammalian PLC isoforms have been identified and classified into 6 families (PLC-β, γ, δ, ε, ζ and η) based on their biochemical properties. Although the expression of PLC isoforms is tissue-specific, concomitant expression of different PLC has been reported, suggesting that PLC family is involved in multiple cellular functions. Despite their critical role, the PLC isoforms expressed in native endothelial cells (ECs) remains undetermined. A conventional PCR approach was initially used to elucidate the mRNA expression pattern of PLC isoforms in 3 distinct murine vascular beds: mesenteric (MA), pulmonary (PA) and middle cerebral arteries (MCA). mRNA encoding for most PLC isoforms was detected in MA, MCA and PA with the exception of η2 and β2 (only expressed in PA), δ4 (only expressed in MCA), η1 (expressed in all but MA) and ζ (not detected in any vascular beds tested). The endothelial-specific PLC expression was then sought in freshly isolated ECs. Interestingly, the PLC expression profile appears to differ across the investigated arterial beds. While mRNA for 8 of the 13 PLC isoforms was detected in ECs from MA, two additional PLC isoforms were detected in ECs from PA and MCA. Co-expression of multiple PLC isoforms in ECs suggests an elaborate network of signalling pathways: PLC isoforms may contribute to the complexity or diversity of signalling by their selective localization in cellular microdomains. However in situ immunofluorescence revealed a homogeneous distribution for all PLC isoforms probed (β3, γ2 and δ1) in intact endothelium. Although PLC isoforms play a crucial role in endothelial signal transduction, subcellular localization alone does not appear to be sufficient to determine the role of PLC in the signalling microdomains found in the

Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells.

Directory of Open Access Journals (Sweden)

Delphine M Béziau

Full Text Available Phospholipase C (PLC comprises a superfamily of enzymes that play a key role in a wide array of intracellular signalling pathways, including protein kinase C and intracellular calcium. Thirteen different mammalian PLC isoforms have been identified and classified into 6 families (PLC-β, γ, δ, ε, ζ and η based on their biochemical properties. Although the expression of PLC isoforms is tissue-specific, concomitant expression of different PLC has been reported, suggesting that PLC family is involved in multiple cellular functions. Despite their critical role, the PLC isoforms expressed in native endothelial cells (ECs remains undetermined. A conventional PCR approach was initially used to elucidate the mRNA expression pattern of PLC isoforms in 3 distinct murine vascular beds: mesenteric (MA, pulmonary (PA and middle cerebral arteries (MCA. mRNA encoding for most PLC isoforms was detected in MA, MCA and PA with the exception of η2 and β2 (only expressed in PA, δ4 (only expressed in MCA, η1 (expressed in all but MA and ζ (not detected in any vascular beds tested. The endothelial-specific PLC expression was then sought in freshly isolated ECs. Interestingly, the PLC expression profile appears to differ across the investigated arterial beds. While mRNA for 8 of the 13 PLC isoforms was detected in ECs from MA, two additional PLC isoforms were detected in ECs from PA and MCA. Co-expression of multiple PLC isoforms in ECs suggests an elaborate network of signalling pathways: PLC isoforms may contribute to the complexity or diversity of signalling by their selective localization in cellular microdomains. However in situ immunofluorescence revealed a homogeneous distribution for all PLC isoforms probed (β3, γ2 and δ1 in intact endothelium. Although PLC isoforms play a crucial role in endothelial signal transduction, subcellular localization alone does not appear to be sufficient to determine the role of PLC in the signalling microdomains found

Arabidopsis phosphoinositide-specific phospholipase C 4 negatively regulates seedling salt tolerance.

Science.gov (United States)

Xia, Keke; Wang, Bo; Zhang, Jiewei; Li, Yuan; Yang, Hailian; Ren, Dongtao

2017-08-01

Previous physiological and pharmacological studies have suggested that the activity of phosphoinositide-specific phospholipase C (PI-PLC) plays an important role in regulating plant salt stress responses by altering the intracellular Ca 2+ concentration. However, the individual members of plant PLCs involved in this process need to be identified. Here, the function of AtPLC4 in the salt stress response of Arabidopsis seedlings was analysed. plc4 mutant seedlings showed hyposensitivity to salt stress compared with Col-0 wild-type seedlings, and the salt hyposensitive phenotype could be complemented by the expression of native promoter-controlled AtPLC4. Transgenic seedlings with AtPLC4 overexpression (AtPLC4 OE) exhibited a salt-hypersensitive phenotype, while transgenic seedlings with its inactive mutant expression (AtPLC4m OE) did not exhibit this phenotype. Using aequorin as a Ca 2+ indicator in plc4 mutant and AtPLC4 OE seedlings, AtPLC4 was shown to positively regulate the salt-induced Ca 2+ increase. The salt-hypersensitive phenotype of AtPLC4 OE seedlings was partially rescued by EGTA. An analysis of salt-responsive genes revealed that the transcription of RD29B, MYB15 and ZAT10 was inversely regulated in plc4 mutant and AtPLC4 OE seedlings. Our findings suggest that AtPLC4 negatively regulates the salt tolerance of Arabidopsis seedlings, and Ca 2+ may be involved in regulating this process. © 2017 John Wiley & Sons Ltd.

Iron-Regulated Phospholipase C Activity Contributes to the Cytolytic Activity and Virulence of Acinetobacter baumannii.

Directory of Open Access Journals (Sweden)

Steven E Fiester

Full Text Available Acinetobacter baumannii is an opportunistic Gram-negative pathogen that causes a wide range of infections including pneumonia, septicemia, necrotizing fasciitis and severe wound and urinary tract infections. Analysis of A. baumannii representative strains grown in Chelex 100-treated medium for hemolytic activity demonstrated that this pathogen is increasingly hemolytic to sheep, human and horse erythrocytes, which interestingly contain increasing amounts of phosphatidylcholine in their membranes. Bioinformatic, genetic and functional analyses of 19 A. baumannii isolates showed that the genomes of each strain contained two phosphatidylcholine-specific phospholipase C (PC-PLC genes, which were named plc1 and plc2. Accordingly, all of these strains were significantly hemolytic to horse erythrocytes and their culture supernatants tested positive for PC-PLC activity. Further analyses showed that the transcriptional expression of plc1 and plc2 and the production of phospholipase and thus hemolytic activity increased when bacteria were cultured under iron-chelation as compared to iron-rich conditions. Testing of the A. baumannii ATCC 19606T plc1::aph-FRT and plc2::aph isogenic insertion derivatives showed that these mutants had a significantly reduced PC-PLC activity as compared to the parental strain, while testing of plc1::ermAM/plc2::aph demonstrated that this double PC-PLC isogenic mutant expressed significantly reduced cytolytic and hemolytic activity. Interestingly, only plc1 was shown to contribute significantly to A. baumannii virulence using the Galleria mellonella infection model. Taken together, our data demonstrate that both PLC1 and PLC2, which have diverged from a common ancestor, play a concerted role in hemolytic and cytolytic activities; although PLC1 seems to play a more critical role in the virulence of A. baumannii when tested in an invertebrate model. These activities would provide access to intracellular iron stores this pathogen

Activation of PLC by an endogenous cytokine (GBP) in Drosophila S3 cells and its application as a model for studying inositol phosphate signalling through ITPK1.

Science.gov (United States)

Zhou, Yixing; Wu, Shilan; Wang, Huanchen; Hayakawa, Yoichi; Bird, Gary S; Shears, Stephen B

2012-12-01

Using immortalized [3H]inositol-labelled S3 cells, we demonstrated in the present study that various elements of the inositol phosphate signalling cascade are recruited by a Drosophila homologue from a cytokine family of so-called GBPs (growth-blocking peptides). HPLC analysis revealed that dGBP (Drosophila GBP) elevated Ins(1,4,5)P3 levels 9-fold. By using fluorescent Ca2+ probes, we determined that dGBP initially mobilized Ca2+ from intracellular pools; the ensuing depletion of intracellular Ca2+ stores by dGBP subsequently activated a Ca2+ entry pathway. The addition of dsRNA (double-stranded RNA) to knock down expression of the Drosophila Ins(1,4,5)P3 receptor almost completely eliminated mobilization of intracellular Ca2+ stores by dGBP. Taken together, the results of the present study describe a classical activation of PLC (phospholipase C) by dGBP. The peptide also promoted increases in the levels of other inositol phosphates with signalling credentials: Ins(1,3,4,5)P4, Ins(1,4,5,6)P4 and Ins(1,3,4,5,6)P5. These results greatly expand the regulatory repertoire of the dGBP family, and also characterize S3 cells as a model for studying the regulation of inositol phosphate metabolism and signalling by endogenous cell-surface receptors. We therefore created a cell-line (S3ITPK1) in which heterologous expression of human ITPK (inositol tetrakisphosphate kinase) was controlled by an inducible metallothionein promoter. We found that dGBP-stimulated S3ITPK1 cells did not synthesize Ins(3,4,5,6)P4, contradicting a hypothesis that the PLC-coupled phosphotransferase activity of ITPK1 [Ins(1,3,4,5,6)P5+Ins(1,3,4)P3→Ins(3,4,5,6)P4+Ins(1,3,4,6)P4] is driven solely by the laws of mass action [Chamberlain, Qian, Stiles, Cho, Jones, Lesley, Grabau, Shears and Spraggon (2007) J. Biol. Chem. 282, 28117-28125]. This conclusion represents a fundamental breach in our understanding of ITPK1 signalling.

Mechanism of enhanced oral absorption of morin by phospholipid complex based self-nanoemulsifying drug delivery system.

Science.gov (United States)

Zhang, Jinjie; Li, Jianbo; Ju, Yuan; Fu, Yao; Gong, Tao; Zhang, Zhirong

2015-02-02

Phospholipid complex (PLC) based self-nanoemulsifying drug delivery system (PLC-SNEDDS) has been developed for efficient delivery of drugs with poor solubility and low permeability. In the present study, a BCS class IV drug and a P-glycoprotein (P-gp) substrate, morin, was selected as the model drug to elucidate the oral absorption mechanism of PLC-SNEDDS. PLC-SNEDDS was superior to PLC in protecting morin from degradation by intestinal enzymes in vitro. In situ perfusion study showed increased intestinal permeability by PLC was duodenum-specific. In contrast, PLC-SNEDDS increased morin permeability in all intestinal segments and induced a change in the main absorption site of morin from colon to ileum. Moreover, ileum conducted the lymphatic transport of PLC-SNEDDS, which was proven by microscopic intestinal visualization of Nile red labeled PLC-SNEDDS and lymph fluids in vivo. Low cytotoxicity and increased Caco-2 cell uptake suggested a safe and efficient delivery of PLC-SNEDDS. The increased membrane fluidity and disrupted actin filaments were closely associated with the increased cell uptake of PLC-SNEDDS. PLC-SNEDDS could be internalized into enterocytes as an intact form in a cholesterol-dependent manner via clathrin-mediated endocytosis and macropinocytosis. The enhanced oral absorption of morin was attributed to the P-gp inhibition by Cremophor RH and the intact internalization of M-PLC-SNEDDS into Caco-2 cells bypassing P-gp recognition. Our findings thus provide new insights into the development of novel nanoemulsions for poorly absorbed drugs.

Intrinsic Pleckstrin Homology (PH) Domain Motion in Phospholipase C-β Exposes a Gβγ Protein Binding Site*

Science.gov (United States)

Kadamur, Ganesh

2016-01-01

Mammalian phospholipase C-β (PLC-β) isoforms are stimulated by heterotrimeric G protein subunits and members of the Rho GTPase family of small G proteins. Although recent structural studies showed how Gαq and Rac1 bind PLC-β, there is a lack of consensus regarding the Gβγ binding site in PLC-β. Using FRET between cerulean fluorescent protein-labeled Gβγ and the Alexa Fluor 594-labeled PLC-β pleckstrin homology (PH) domain, we demonstrate that the PH domain is the minimal Gβγ binding region in PLC-β3. We show that the isolated PH domain can compete with full-length PLC-β3 for binding Gβγ but not Gαq, Using sequence conservation, structural analyses, and mutagenesis, we identify a hydrophobic face of the PLC-β PH domain as the Gβγ binding interface. This PH domain surface is not solvent-exposed in crystal structures of PLC-β, necessitating conformational rearrangement to allow Gβγ binding. Blocking PH domain motion in PLC-β by cross-linking it to the EF hand domain inhibits stimulation by Gβγ without altering basal activity or Gαq response. The fraction of PLC-β cross-linked is proportional to the fractional loss of Gβγ response. Cross-linked PLC-β does not bind Gβγ in a FRET-based Gβγ-PLC-β binding assay. We propose that unliganded PLC-β exists in equilibrium between a closed conformation observed in crystal structures and an open conformation where the PH domain moves away from the EF hands. Therefore, intrinsic movement of the PH domain in PLC-β modulates Gβγ access to its binding site. PMID:27002154

20(S-Protopanaxadiol Phospholipid Complex: Process Optimization, Characterization, In Vitro Dissolution and Molecular Docking Studies

Directory of Open Access Journals (Sweden)

Yiqiong Pu

2016-10-01

Full Text Available 20(S-Protopanaxadiol (PPD, a bioactive compound extracted from ginseng, possesses cardioprotective, neuroprotective, anti-inflammatory, antiestrogenic, anticancer and anxiolytic effects. However, the clinical application of PPD is limited by its weak aqueous solubility. In this study, we optimized an efficient method of preparing its phospholipid complex (PPD-PLC using a central composite design and response surface analysis. The prepared PPD-PLC was characterized by differential scanning calorimetric, powder X-ray diffraction, Fourier-transformed infrared spectroscopy and nuclear magnetic resonance analyses associated with molecular docking calculation. The equilibrium solubility of PPD-PLC in water and n-octanol increased 6.53- and 1.53-times, respectively. Afterwards, using PPD-PLC as the intermediate, the PPD-PLC-loaded dry suspension (PPD-PLC-SU was prepared with our previous method. In vitro evaluations were conducted on PPD-PLC and PPD-PLC-SU, including dissolution behaviors and stability properties under different conditions. Results of in vitro dissolution behavior revealed the improved dissolution extents and rates of PPD-PLC and PPD-PLC-SU (p < 0.05. Results of the formulation stability investigation also exposed the better stability of PPD-PLC-SU compared with free PPD. Therefore, phospholipid complex technology is a useful formulation strategy for BCS II drugs, as it could effectively improve their hydrophilicity and lipophilicity.

Intrinsic Pleckstrin Homology (PH) Domain Motion in Phospholipase C-β Exposes a Gβγ Protein Binding Site.

Science.gov (United States)

Kadamur, Ganesh; Ross, Elliott M

2016-05-20

Mammalian phospholipase C-β (PLC-β) isoforms are stimulated by heterotrimeric G protein subunits and members of the Rho GTPase family of small G proteins. Although recent structural studies showed how Gαq and Rac1 bind PLC-β, there is a lack of consensus regarding the Gβγ binding site in PLC-β. Using FRET between cerulean fluorescent protein-labeled Gβγ and the Alexa Fluor 594-labeled PLC-β pleckstrin homology (PH) domain, we demonstrate that the PH domain is the minimal Gβγ binding region in PLC-β3. We show that the isolated PH domain can compete with full-length PLC-β3 for binding Gβγ but not Gαq, Using sequence conservation, structural analyses, and mutagenesis, we identify a hydrophobic face of the PLC-β PH domain as the Gβγ binding interface. This PH domain surface is not solvent-exposed in crystal structures of PLC-β, necessitating conformational rearrangement to allow Gβγ binding. Blocking PH domain motion in PLC-β by cross-linking it to the EF hand domain inhibits stimulation by Gβγ without altering basal activity or Gαq response. The fraction of PLC-β cross-linked is proportional to the fractional loss of Gβγ response. Cross-linked PLC-β does not bind Gβγ in a FRET-based Gβγ-PLC-β binding assay. We propose that unliganded PLC-β exists in equilibrium between a closed conformation observed in crystal structures and an open conformation where the PH domain moves away from the EF hands. Therefore, intrinsic movement of the PH domain in PLC-β modulates Gβγ access to its binding site. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Recent research progress with phospholipase C from Bacillus cereus.

Science.gov (United States)

Lyu, Yan; Ye, Lidan; Xu, Jun; Yang, Xiaohong; Chen, Weiwei; Yu, Hongwei

2016-01-01

Phospholipase C (PLC) catalyzes the hydrolysis of phospholipids to produce phosphate monoesters and diacylglycerol. It has many applications in the enzymatic degumming of plant oils. PLC Bc , a bacterial PLC from Bacillus cereus, is an optimal choice for this activity in terms of its wide substrate spectrum, high activity, and approved safety. Unfortunately, its large-scale production and reliable high-throughput screening of PLC Bc remain challenging. Herein, we summarize the research progress regarding PLC Bc with emphasis on the screening methods, expression systems, catalytic mechanisms and inhibitor of PLC Bc . This review hopefully will inspire new achievements in related areas, to promote the sustainable development of PLC Bc and its application.

Characterization of phospholipase C gamma enzymes with gain-of-function mutations.

Science.gov (United States)

Everett, Katy L; Bunney, Tom D; Yoon, Youngdae; Rodrigues-Lima, Fernando; Harris, Richard; Driscoll, Paul C; Abe, Koichiro; Fuchs, Helmut; de Angelis, Martin Hrabé; Yu, Philipp; Cho, Wohnwa; Katan, Matilda

2009-08-21

Phospholipase C gamma isozymes (PLC gamma 1 and PLC gamma 2) have a crucial role in the regulation of a variety of cellular functions. Both enzymes have also been implicated in signaling events underlying aberrant cellular responses. Using N-ethyl-N-nitrosourea (ENU) mutagenesis, we have recently identified single point mutations in murine PLC gamma 2 that lead to spontaneous inflammation and autoimmunity. Here we describe further, mechanistic characterization of two gain-of-function mutations, D993G and Y495C, designated as ALI5 and ALI14. The residue Asp-993, mutated in ALI5, is a conserved residue in the catalytic domain of PLC enzymes. Analysis of PLC gamma 1 and PLC gamma 2 with point mutations of this residue showed that removal of the negative charge enhanced PLC activity in response to EGF stimulation or activation by Rac. Measurements of PLC activity in vitro and analysis of membrane binding have suggested that ALI5-type mutations facilitate membrane interactions without compromising substrate binding and hydrolysis. The residue mutated in ALI14 (Tyr-495) is within the spPH domain. Replacement of this residue had no effect on folding of the domain and enhanced Rac activation of PLC gamma 2 without increasing Rac binding. Importantly, the activation of the ALI14-PLC gamma 2 and corresponding PLC gamma 1 variants was enhanced in response to EGF stimulation and bypassed the requirement for phosphorylation of critical tyrosine residues. ALI5- and ALI14-type mutations affected basal activity only slightly; however, their combination resulted in a constitutively active PLC. Based on these data, we suggest that each mutation could compromise auto-inhibition in the inactive PLC, facilitating the activation process; in addition, ALI5-type mutations could enhance membrane interaction in the activated state.

Identification of the Elusive Mammalian Enzyme Phosphatidylcholine-Specific Phospholipase C

Science.gov (United States)

2015-09-01

Summary of Results. Task 1. To identify mammalian PC- PLC . Based on results published by other groups, we proposed to identify candidate PC- PLC mRNAs by...establishing the role of the elusive mammalian protein, phosphatidycholine- specific phospholipase C (PC- PLC ) in the inflammatory processes involved in...progression of rheumatoid arthritis (RA). Thus, the main scopes of this proposal are: 1. to identify the PC- PLC gene and protein; and 2. to test PC- PLC

Frequency domain based LS channel estimation in OFDM based Power line communications

OpenAIRE

Bogdanović, Mario

2015-01-01

This paper is focused on low voltage power line communication (PLC) realization with an emphasis on channel estimation techniques. The Orthogonal Frequency Division Multiplexing (OFDM) scheme is preferred technology in PLC systems because of its effective combat with frequency selective fading properties of PLC channel. As the channel estimation is one of the crucial problems in OFDM based PLC system because of a problematic area of PLC signal attenuation and interference, the improved LS est...

MR imaging of the posterolateral corner of the knee

Energy Technology Data Exchange (ETDEWEB)

Bolog, Nicolae [Emergency Hospital, Department of Radiology, Bucharest (Romania); Hodler, Juerg [Orthopaedic University Hospital Balgrist, Department of Radiology, Zurich (Switzerland)

2007-08-15

The posterolateral corner (PLC) is a complex functional unit, consisting of several structures, which is responsible for posterolateral stabilization. The PLC is not consistently defined in the literature. However, most descriptions include the popliteal tendon (PT), the lateral collateral ligament (LCL), the popliteofibular ligament (PFL) and the posterolateral capsule, which is reinforced by the arcuate ligament (AL) and the fabellofibular ligament (FFL). Knowledge of PLC anatomy, including its variations, and understanding of the biomechanics is important for correct diagnosis of PLC injuries. An overlooked PLC injury can result in chronic instability, chronic pain, and, eventually, in secondary osteoarthritis. Damage to the PLC also has an adverse effect on the outcome of cruciate ligament repair. Isolated lesions of the PLC are rare. PLC lesions are typically associated with injuries of the cruciate ligaments, the menisci, bone and soft tissue. In the acute phase, clinical findings can be difficult to interpret due to pain and swelling. Magnetic resonance (MR) imaging potentially demonstrates the entire spectrum of PLC injuries and associated lesions of the knee, including those that may be overlooked during clinical examination or arthroscopy. (orig.)

Presenilin dependence of phospholipase C and protein kinase C signaling

DEFF Research Database (Denmark)

Dehvari, Nodi; Cedazo-Minguez, Angel; Isacsson, Ola

2007-01-01

-stimulated phospholipase C (PLC) activity which was gamma-secretase dependent. To further evaluate the dependence of PLC on PSs we measured PLC activity and the activation of variant protein kinase C (PKC) isoforms in mouse embryonic fibroblasts (MEFs) lacking either PS1, PS2, or both. PLC activity and PKCalpha...

Chapter 1. The structure of the company

International Nuclear Information System (INIS)

1998-01-01

In the first chapter of this CD ROM the structure of the Slovak Electric, Plc. (Slovenske elektrarne, a.s.) in 1998 is presented. It consist of next paragraphs (1) The history (The origin of the SE, Plc.; Main events of 1995; Main events of 1996; Main events of 1997); (2) The bodies of SE, Plc. (General Meeting of Shareholders; Supervisory Board; Board of Directors); (3) Organizational structure of the the Company (The Headquarters of SE, Plc.; SE, Plc, Transmission System)

Variation in sister chromatid exchange frequencies between human and pig whole blood, plasma leukocyte, and mononuclear leukocyte cultures

International Nuclear Information System (INIS)

Larramendy, M.L.; Reigosa, M.A.

1986-01-01

Sister chromatid exchange (SCE) induction by ultraviolet (UV) light was studied in both human and pig whole blood cultures (WBC) and plasma leukocyte cultures (PLC). No variation in SCE frequency was observed between pig WBC and PLC in control as well as in treated cells. Conversely, SCE frequencies of human PLC were consistently higher than those of WBC in control and UV-exposed cells. Thus, red blood cells (RBCs) do not influence the sensitivity of lymphocytes to UV LIGHT exposure, and there must be some different culture condition(s) in the inducation of SCEs between human WBC and PLC but not in swine lymphocyte cultures. Since the BrdUrd/lymphocyte ratio of WBC was halved in PLC, the effect of BrdUrd concentration in inducing the SCE baseline frequency of PLC may be ruled out. Neither the cell separation technique nor polymorphonuclear leukocytes had a significant role in the elevated SCE frequency of human PLC or MLC. Experiments where human RBCs were titrated into human PLC showed that the induction of an elevated SCE frequency of PLC was suppressed in a dose-dependent manner by the presence of RBCs in the culture medium. Since the incorporation of pig or human RBCs into human PLC as well as into MLC reduced the SCE frequency to that of WBC, a common component and/or function existing in these cells is suggested. Analysis of different RBC components showed that RBCs, specifically RBC ghosts, release a diffusible but not dialyzable corrective factor into culture medium that is able to reduce the SCE frequencies of PLC

Plant phospholipase C family: Regulation and functional role in lipid signaling.

Science.gov (United States)

Singh, Amarjeet; Bhatnagar, Nikita; Pandey, Amita; Pandey, Girdhar K

2015-08-01

Phospholipase C (PLC), a major membrane phospholipid hydrolyzing enzyme generates signaling messengers such as diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) in animals, and their phosphorylated forms such as phosphatidic acid (PA) and inositol hexakisphosphate (IP6) are thought to regulate various cellular processes in plants. Based on substrate specificity, plant PLC family is sub-divided into phosphatidylinositol-PLC (PI-PLC) and phosphatidylcholine-PLC (PC-PLC) groups. The activity of plant PLCs is regulated by various factors and the major ones include, Ca(2+) concentration, phospholipid substrate, post-translational modifications and interacting proteins. Most of the PLC members have been localized at the plasma membrane, suited for their function of membrane lipid hydrolysis. Several PLC members have been implicated in various cellular processes and signaling networks, triggered in response to a number of environmental cues and developmental events in different plant species, which makes them potential candidates for genetically engineering the crop plants for stress tolerance and enhancing the crop productivity. In this review article, we are focusing mainly on the plant PLC signaling and regulation, potential cellular and physiological role in different abiotic and biotic stresses, nutrient deficiency, growth and development. Copyright © 2015 Elsevier Ltd. All rights reserved.

PERBANDINGAN JENIS MEDIA TANAM TANAH ENTISOL YANG TERPAPAR SATU KALI DAN DUA KALI OLEH LIMBAH CAIR NANAS TERHADAP PERTUMBUHAN SELEDRI (Apium graveolens L. SEBAGAI SUMBER BELAJAR BIOLOGI

Directory of Open Access Journals (Sweden)

Irma Yunita Sari Kartika Sari

2015-10-01

Full Text Available Abstract. This research to know effect of Pineapple Liquid Compost (PLC in long term the growth of celery. This research conducted at the village in Yosodadi 21 Polos, East Metro, Metro City since 7 December until 28 February 2015. This research is experimental research, the design used the Complete Random Design (CRD. This research used entisol soil that was exposed by PLC once and entisol soil that was exposed by PLC twice. The treatment was done to them both by giving PLC about 200ml in a time per three days. The data analysis was done by using normality test (liliefors, homogeneity test (barlett and Ttes. It shows that there is no negative effect of fertilizer use PLC in the long term against the growth of celery in entisol soil that was exposed by PLC once and entisol soil that was exposed by PLC twice.

Insulin-like growth factor-binding protein 7 alters the sensitivity to interferon-based anticancer therapy in hepatocellular carcinoma cells.

Science.gov (United States)

Tomimaru, Y; Eguchi, H; Wada, H; Noda, T; Murakami, M; Kobayashi, S; Marubashi, S; Takeda, Y; Tanemura, M; Umeshita, K; Doki, Y; Mori, M; Nagano, H

2010-05-11

A striking efficiency of interferon (IFN)-based anticancer therapy for advanced hepatocellular carcinoma (HCC) has been reported. Because its clinical efficiency greatly depends on each patient's local response, prediction of local response is crucial. Continuous exposure of IFN-alpha to parental PLC/PRF/5 cells (PLC-P) and a limiting dilution method resulted in the establishment of IFN-resistant cell clones (PLC-Rs). Microarray analyses of PLC-P and PLC-Rs identified insulin-like growth factor-binding protein 7 (IGFBP7) as one of the most significantly downregulated genes in PLC-Rs. Changes in anticancer effects of IFN-alpha were examined in HCC cells after genetic manipulation of IGFBP7 expression. The correlation between immunohistochemically determined IGFBP7 expression and the response to IFN-alpha/5-fluorouracil (5-FU) therapy was investigated in surgically resected HCC specimens. PLC-R cells showed a remarkable downregulation of IGFBP7 and resistance to IFN-alpha, compared with PLC-P. Parental PLC/PRF/5 cells transfected with short hairpin RNA against IGFBP7 showed a significant resistance to IFN-alpha relative to control cells (IC(50) fold increase=14.38 times). Insulin-like growth factor-binding protein 7 transfection into PLC-R restored sensitivity to IFN-alpha. In resected specimens, IGFBP7 expression significantly correlated with the response to IFN-alpha/5-FU therapy. IGFBP7 could be a useful predictor of the response to IFN-based therapy in advanced HCC.

Phosphatidic acid regulates signal output by G protein coupled receptors through direct interaction with phospholipase C-beta(1).

Science.gov (United States)

Litosch, Irene; Pujari, Rajeshree; Lee, Shawn J

2009-09-01

Phosphatidic acid (PA), generated downstream of monomeric Rho GTPases via phospholipase D (PLD) and additionally by diacylglycerol kinases (DGK), both stimulates phospholipase C-beta(1) (PLC-beta(1)) and potentiates stimulation of PLC-beta(1) activity by Galpha(q) in vitro. PA is a potential candidate for integrating signaling by monomeric and heterotrimeric G proteins to regulate signal output by G protein coupled receptors (GPCR), and we have sought to understand the mechanisms involved. We previously identified the region spanning residues 944-957, lying within the PLC-beta(1) C-terminus alphaA helix and flexible loop of the Galpha(q) binding domain, as required for stimulation of lipase activity by PA in vitro. Regulation by PA does not require residues essential for stimulation by Galpha(q) or GTPase activating activity. The present studies evaluated shorter alanine/glycine replacement mutants and finally point mutations to identify Tyr(952) and Ile(955) as key determinants for regulation by PA, assessed by both in vitro enzymatic and cell-based co-transfection assays. Replacement of Tyr(952) and Ile(955), PLC-beta(1) (Y952G/I955G), results in an 85% loss in stimulation by PA relative to WT-PLC-beta(1) in vitro. COS 7 cells co-transfected with PLC-beta(1) (Y952G/I955G) demonstrate a 10-fold increase in the EC(50) for stimulation and a 60% decrease in maximum stimulation by carbachol via Galpha(q) linked m1 muscarinic receptors, relative to cells co-transfected with WT-PLC-beta(1) but otherwise similar conditions. Residues required for regulation by PA are not essential for stimulation by G protein subunits. WT-PLC-beta(1) and PLC-beta(1) (Y952G/I955G) activity is increased comparably by co-transfection with Galpha(q) and neither is markedly affected by co-transfection with Gbeta(1)gamma(2). Inhibiting PLD-generated PA production by 1-butanol has little effect on maximum stimulation, but shifts the EC(50) for agonist stimulation of WT-PLC-beta(1) by 10-fold

The binding of activated Gαq to phospholipase C-β exhibits anomalous affinity.

Science.gov (United States)

Navaratnarajah, Punya; Gershenson, Anne; Ross, Elliott M

2017-10-06

Upon activation by the G q family of Gα subunits, Gβγ subunits, and some Rho family GTPases, phospholipase C-β (PLC-β) isoforms hydrolyze phosphatidylinositol 4,5-bisphosphate to the second messengers inositol 1,4,5-trisphosphate and diacylglycerol. PLC-β isoforms also function as GTPase-activating proteins, potentiating G q deactivation. To elucidate the mechanism of this mutual regulation, we measured the thermodynamics and kinetics of PLC-β3 binding to Gα q FRET and fluorescence correlation spectroscopy, two physically distinct methods, both yielded K d values of about 200 nm for PLC-β3-Gα q binding. This K d is 50-100 times greater than the EC 50 for Gα q -mediated PLC-β3 activation and for the Gα q GTPase-activating protein activity of PLC-β. The measured K d was not altered either by the presence of phospholipid vesicles, phosphatidylinositol 4,5-bisphosphate and Ca 2+ , or by the identity of the fluorescent labels. FRET-based kinetic measurements were also consistent with a K d of 200 nm We determined that PLC-β3 hysteresis, whereby PLC-β3 remains active for some time following either Gα q -PLC-β3 dissociation or PLC-β3-potentiated Gα q deactivation, is not sufficient to explain the observed discrepancy between EC 50 and K d These results indicate that the mechanism by which Gα q and PLC-β3 mutually regulate each other is far more complex than a simple, two-state allosteric model and instead is probably kinetically determined. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

77 FR 76156 - Self-Regulatory Organizations; ICE Clear Credit LLC; Notice of Filing of Proposed Rule Change To...

Science.gov (United States)

2012-12-26

... public limited company; COMPAGNIE DE SAINT-GOBAIN; Deutsche Telekom AG; FRANCE TELECOM; GAS NATURAL SDG... GUICHARD- PERRACHON; COMPASS GROUP PLC; EXPERIAN FINANCE PLC; GROUPE AUCHAN; J SAINSBURY plc; Koninklijke...

Implementation of the resonant vibratory feeders control algorithm on Simatic S7-1200 from MATLAB Simulink enviroment

Directory of Open Access Journals (Sweden)

Mitrović Radomir B.

2016-01-01

Full Text Available Simulink is an important tool for modeling and simulation of process and control algorithms. It's expansion, PLC Coder, enables direct conversion of model subsystem into SCL, structured text code, which is then used by PLC IDE to create function blocks. This shortens developing time of algorithms for PLC controller. Also, this reduces possibility for a coding error. This paper describes Simulink PLC Coder and workflow for developing PID control algorithm for Siemens Simatic S7-1200 PLC. Control object used here is resonant vibratory feeder having electromagnetic drive.

Adaptation of Powerline Communications-Based Smart Metering Deployments to the Requirements of Smart Grids

Directory of Open Access Journals (Sweden)

Alberto Sendin

2015-11-01

Full Text Available Powerline communications (PLC-based smart meter deployments are now a reality in many regions of the world. Although PLC elements are generally incorporated in smart meters and data concentrators, the underlying PLC network allows the integration of other smart grid services directly over it. The remote control capabilities that automation programs need and are today deployed over their medium voltage (MV grid, can be extended to the low voltage (LV grid through these existing PLC networks. This paper demonstrates the capabilities of narrowband high data rate (NB HDR PLC technologies deployed over LV grids for smart metering purposes to support internet protocol internet protocol (IP communications in the LV grid. The paper demonstrates these possibilities with the presentation of the simulation and laboratory results of IP communications over international telecommunication union: ITU-T G.9904 PLC technology, and the definition of a PLC Network Management System based on a simple network management protocol (SNMP management information base (MIB definition and applicable use cases.

Diseño e implementación de un sistema de transferencias de datos a través de la red eléctrica de baja tensión con la interfaz Labview-arduino empleando la tecnología power line communications (PLC).

OpenAIRE

Ponce León, David; Santillán Aguirre, Sergio

2016-01-01

La propuesta del proyecto de titulación está basada en la implementación de un nuevo sistema de transferencia mediante tecnología Power Line Communications (PLC), Comunicación por la Red Eléctrica, que se fundamenta en la transmisión de información en diferentes puntos utilizando la red eléctrica ya existente de baja tensión en el Laboratorio de Telecomunicaciones, con la finalidad de contribuir con nuevas opciones de comunicación, de envío y recepción de información para aplicaciones tecnoló...

Dynamics of receptor-operated Ca2+ Currents Through TRPC Channels Controlled via the PI(4,5P2-PLC Signaling Pathway

Directory of Open Access Journals (Sweden)

Masayuki X Mori

2015-02-01

Full Text Available Transient receptor potential canonical (TRPC channels are Ca2+-permeable, nonselective cation channels that carry receptor-operated Ca2+ currents (ROCs triggered by receptor-induced, phospholipase C (PLC-catalyzed hydrolysis of phosphatidylinositol 4,5-bisphosphate (PI(4,5P2. Within the vasculature, TRPC channel ROCs contribute to smooth muscle cell depolarization, vasoconstriction and vascular remodeling. However, TRPC channel ROCs exhibit a variable response to receptor-stimulation, and the regulatory mechanisms governing TRPC channel activity remain obscure. The variability of ROCs may be explained by their complex regulation by PI(4,5P2 and its metabolites, which differentially affect TRPC channel activity. To resolve the complex regulation of ROCs, the use of voltage-sensing phosphoinositide phosphatases and model simulation have helped to reveal the time-dependent contribution of PI(4,5P2 and the possible role of PI(4,5P2 in the regulation of ROCs. These approaches may provide unprecedented insight into the dynamics of PI(4,5P2 regulation of TRPC channels and the fundamental mechanisms underlying transmembrane ion flow. Within that context, we summarize the regulation of TRPC channels and their coupling to receptor-mediated signaling, as well as the application of voltage-sensing phosphoinositide phosphatases to this research. We also discuss the controversial bidirectional effects of PI(4,5P2 using a model simulation that could explain the complicated effects of PI(4,5P2 on different ROCs.

Dynamics of receptor-operated Ca(2+) currents through TRPC channels controlled via the PI(4,5)P2-PLC signaling pathway.

Science.gov (United States)

Mori, Masayuki X; Itsuki, Kyohei; Hase, Hideharu; Sawamura, Seishiro; Kurokawa, Tatsuki; Mori, Yasuo; Inoue, Ryuji

2015-01-01

Transient receptor potential canonical (TRPC) channels are Ca(2+)-permeable, nonselective cation channels that carry receptor-operated Ca(2+) currents (ROCs) triggered by receptor-induced, phospholipase C (PLC)-catalyzed hydrolysis of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]. Within the vasculature, TRPC channel ROCs contribute to smooth muscle cell depolarization, vasoconstriction, and vascular remodeling. However, TRPC channel ROCs exhibit a variable response to receptor-stimulation, and the regulatory mechanisms governing TRPC channel activity remain obscure. The variability of ROCs may be explained by their complex regulation by PI(4,5)P2 and its metabolites, which differentially affect TRPC channel activity. To resolve the complex regulation of ROCs, the use of voltage-sensing phosphoinositide phosphatases and model simulation have helped to reveal the time-dependent contribution of PI(4,5)P2 and the possible role of PI(4,5)P2 in the regulation of ROCs. These approaches may provide unprecedented insight into the dynamics of PI(4,5)P2 regulation of TRPC channels and the fundamental mechanisms underlying transmembrane ion flow. Within that context, we summarize the regulation of TRPC channels and their coupling to receptor-mediated signaling, as well as the application of voltage-sensing phosphoinositide phosphatases to this research. We also discuss the controversial bidirectional effects of PI(4,5)P2 using a model simulation that could explain the complicated effects of PI(4,5)P2 on different ROCs.

A non-catalytic histidine residue influences the function of the metalloprotease of Listeria monocytogenes.

Science.gov (United States)

Forster, Brian M; Bitar, Alan Pavinski; Marquis, Hélène

2014-01-01

Mpl, a thermolysin-like metalloprotease, and PC-PLC, a phospholipase C, are synthesized as proenzymes by the intracellular bacterial pathogen Listeria monocytogenes. During intracellular growth, L. monocytogenes is temporarily confined in a membrane-bound vacuole whose acidification leads to Mpl autolysis and Mpl-mediated cleavage of the PC-PLC N-terminal propeptide. Mpl maturation also leads to the secretion of both Mpl and PC-PLC across the bacterial cell wall. Previously, we identified negatively charged and uncharged amino acid residues within the N terminus of the PC-PLC propeptide that influence the ability of Mpl to mediate the maturation of PC-PLC, suggesting that these residues promote the interaction of the PC-PLC propeptide with Mpl. In the present study, we identified a non-catalytic histidine residue (H226) that influences Mpl secretion across the cell wall and its ability to process PC-PLC. Our results suggest that a positive charge at position 226 is required for Mpl functions other than autolysis. Based on the charge requirement at this position, we hypothesize that this residue contributes to the interaction of Mpl with the PC-PLC propeptide.

Tailoring the mechanical and biodegradable properties of binary blends of biomedical thermoplastic elastomer.

Science.gov (United States)

Ang, Hui Ying; Chan, Jingni; Toong, Daniel; Venkatraman, Subbu S; Chia, Sing Joo; Huang, Ying Ying

2018-03-01

Blending polymers with complementary properties capitalizes on the inherent advantages of both components, making it possible to tailor the behaviour of the resultant material. A polymer blend consisting of an elastomer and thermoplastic can help to improve the mechanical integrity of the system without compromising on its processibility. A series of blends of biodegradable Poly(L-lactide-co-ɛ-caprolactone) (PLC) and Poly-(l,l-lactide-co-glycolic acid) (PLLGA), and PLC with Poly-(d,l-lactide-co-glycolic acid) (PDLLGA) were evaluated as a potential material for a biodegradable vesicourethral connector device. Based on the Tg of the blends, PLC/PLLGA formed an immiscible mixture while PLC/PDLLGA resulted in a compatible blend. The results showed that with the blending of PLC, the failure mode of PLLGA and PDLLGA changed from brittle to ductile fracture, with an significant decreas in tensile modulus and strength. SEM images demonstrated the different blend morphologies of different compositions during degradation. Gel Permeation Chromatography (GPC) and mechanical characterization revealed the degradation behaviour of the blends in this order (fastest to slowest): PDLLGA and PLC/PDLLGA blends > PLLGA and PLC/PLLGA blends > PLC. The PLC/PLLGA (70:30) blend was recommended as a suitable for the vesicourethral connector device application, highlighting the tailoring of blends to achieve a desired mechanical performance. Copyright © 2017 Elsevier Ltd. All rights reserved.

Phospholipase C produced by Clostridium botulinum types C and D: comparison of gene, enzymatic, and biological activities with those of Clostridium perfringens alpha-toxin.

Science.gov (United States)

Fatmawati, Ni Nengah Dwi; Sakaguchi, Yoshihiko; Suzuki, Tomonori; Oda, Masataka; Shimizu, Kenta; Yamamoto, Yumiko; Sakurai, Jun; Matsushita, Osamu; Oguma, Keiji

2013-01-01

Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar plates. To characterize the gene, enzymatic and biological activities of C. botulinum PLCs (Cb-PLCs), the cb-plc genes from 8 strains were sequenced, and 1 representative gene was cloned and expressed as a recombinant protein. The enzymatic and hemolytic activities of the recombinant Cb-PLC were measured and compared with those of the Clostridium perfringens alpha-toxin. Each of the eight cb-plc genes encoded a 399 amino acid residue protein preceded by a 27 residue signal peptide. The protein consists of 2 domains, the N- and C-domains, and the overall amino acid sequence identity between Cb-PLC and alpha-toxin was greater than 50%, suggesting that Cb-PLC is homologous to the alpha-toxin. The key residues in the N-domain were conserved, whereas those in the C-domain which are important in membrane interaction were different than in the alpha-toxin. As expected, Cb-PLC could hydrolyze egg yolk phospholipid, p-nitrophenylphosphorylcholine, and sphingomyelin, and also exhibited hemolytic activity;however, its activities were about 4- to over 200-fold lower than those of alpha-toxin. Although Cb-PLC showed weak enzymatic and biological activities, it is speculated that Cb-PLC might play a role in the pathogenicity of botulism or for bacterial survival.

Investigating the neuroanatomical substrate of pathological laughing and crying in amyotrophic lateral sclerosis with multimodal neuroimaging techniques.

Science.gov (United States)

Christidi, Foteini; Karavasilis, Efstratios; Ferentinos, Panagiotis; Xirou, Sophia; Velonakis, Georgios; Rentzos, Michalis; Zouvelou, Vasiliki; Zalonis, Ioannis; Efstathopoulos, Efstathios; Kelekis, Nikolaos; Evdokimidis, Ioannis

2018-02-01

Pathological laughing and crying (PLC) is common in several neurological and psychiatric diseases and is associated with a distributed network involving the frontal cortex, the brainstem and cortico-pontine-cerebellar circuits. By applying multimodal neuroimaging approach, we examined the neuroanatomical substrate of PLC in a sample of patients with amyotrophic lateral sclerosis (ALS). We studied 56 non-demented ALS patients and 25 healthy controls (HC). PLC was measured in ALS using the Center of Neurologic Study Lability Scale (CNS-LS; cutoff score: 13). All participants underwent 3D-T1-weighted and 30-directional diffusion-weighted imaging at 3T. Voxel-based morphometry and tract-based spatial-statistics analysis was used to examine gray matter (GM) and white matter (WM) differences between ALS patients with and without PLC (ALS-PLC and ALS-nonPLC, respectively). Comparisons were restricted to regions with detected differences between ALS and HC, controlling for age, gender, total intracranial volume and depressive symptoms. In regions with significant differences between ALS and HC, ALS-PLC patients showed decreased GM volume in left orbitofrontal cortex, frontal operculum, and putamen and bilateral frontal poles, compared to ALS-nonPLC. They also had decreased fractional anisotropy in left cingulum bundle and posterior corona radiata. WM abnormalities were additionally detected in WM associative and ponto-cerebellar tracts (using a more liberal threshold). PLC in ALS is driven by both GM and WM abnormalities which highlight the role of circuits rather than isolated centers in the emergence of this condition. ALS is suggested as a useful natural experimental model to study PLC.

No effect of growth hormone administration on substrate oxidation during exercise in young, lean men

DEFF Research Database (Denmark)

Hansen, Mette; Morthorst, Rikke; Larsson, Benny

2005-01-01

) (completed four randomised trials: 120 min bicycling at 55% 4 h after receiving either 7.5 IU (2.5 mg) GH or placebo (Plc), and during rest after receiving either GH or Plc. In all studies a standardized meal was given 2 h after GH or Plc injection. GH administration resulted in an approximately 60-fold...... increase in serum GH concentration at rest (P Plc...

Salicylic acid modulates levels of phosphoinositide dependent-phospholipase C substrates and products to remodel the Arabidopsis suspension cell transcriptome

Directory of Open Access Journals (Sweden)

Eric eRuelland

2014-11-01

Full Text Available Basal phosphoinositide-dependent phospholipase C (PI-PLC activity controls gene expression in Arabidopsis suspension cells and seedlings. PI-PLC catalyzes the production of phosphorylated inositol and diacylglycerol (DAG from phosphoinositides. It is not known how PI-PLC regulates the transcriptome although the action of DAG-kinase (DGK on DAG immediately downstream from PI-PLC is responsible for some of the regulation. We previously established a list of genes whose expression is affected in the presence of PI-PLC inhibitors. Here this list of genes was used as a signature in similarity searches of curated plant hormone response transcriptome data. The strongest correlations obtained with the inhibited PI-PLC signature were with salicylic acid (SA treatments. We confirm here that in Arabidopsis suspension cells SA treatment leads to an increase in phosphoinositides, then demonstrate that SA leads to a significant 20% decrease in phosphatidic acid, indicative of a decrease in PI-PLC products. Previous sets of microarray data were re-assessed. The SA response of one set of genes was dependent on phosphoinositides. Alterations in the levels of a second set of genes, mostly SA-repressed genes, could be related to decreases in PI-PLC products that occur in response to SA action. Together, the two groups of genes comprise at least 40% of all SA-responsive genes. Overall these two groups of genes are distinct in the functional categories of the proteins they encode, their promoter cis-elements and their regulation by DGK or phospholipase D. SA-regulated genes dependent on phosphoinositides are typical SA response genes while those with an SA response that is possibly dependent on PI-PLC products are less SA-specific. We propose a model in which SA inhibits PI-PLC activity and alters levels of PI-PLC products and substrates, thereby regulating gene expression divergently.

Prospective analysis of magnetic resonance imaging accuracy in diagnosing traumatic injuries of the posterior ligamentous complex of the thoracolumbar spine.

Science.gov (United States)

Pizones, Javier; Sánchez-Mariscal, Felisa; Zúñiga, Lorenzo; Álvarez, Patricia; Izquierdo, Enrique

2013-04-20

Prospective cohort study. To study magnetic resonance imaging (MRI) accuracy in diagnosing posterior ligamentous complex (PLC) damage, when applying the new dichotomic instability criteria in a prospective cohort of patients with vertebral fracture. Recent studies dispute MRI accuracy to diagnose PLC injuries. They analyze the complex based on 3 categories (intact/indeterminate/rupture), including the indeterminate in the ruptured group (measurement bias) in the accuracy analysis. Moreover, fractures with conservative treatment (selection bias) are not included. Both facts reduce the specificity. A recent study has proposed new criteria where posterior instability is determined with supraspinous ligament (SSL) rupture. Prospective study of patients with acute thoracolumbar fracture, using radiography and MRI (FS-T2-w/short-tau inversion-recovery sequences). 1. The integrity (ruptured/unruptured) of each isolated component of the PLC (facet capsules, interspinous ligament, SSL, and ligamentum flavum) was assessed via MRI and surgical findings. 2. PLC integrity as a whole was assessed, adopting the new dichotomic stability criteria from previous studies. In the MR images, PLC is considered ruptured when the SSL is found discontinued, and intact when not (this excludes the "indeterminate" category). In surgically treated fractures, PLC stability as a whole was assessed dynamically (ruptured/unruptured). In conservative fractures, PLC stability was assessed according to change in vertebral kyphosis measured with the local kyphotic angle at 2-year follow-up (ruptured if difference is > 5°/unruptured if difference is PLC damage. Fifty-eight vertebral fractures were studied (38 surgical, 20 conservative), of which 50% were in males; average age, 40.4 years. MRI sensitivity for injury diagnosis of each isolated PLC component varied between 92.3% (interspinous ligament) and 100% (ligamentum flavum). Specificity varied between 52% (facet capsules) and 100% (SSL). PLC

Kapak ve İçindekiler

Directory of Open Access Journals (Sweden)

Kapak ve İçindekiler

2015-12-01

Full Text Available Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Chemical Abstracts Service , EBSCO Discovery Service, Google Scholar, WorldCat ve Zentralblatt MATH indekslerinde taranmaktadır.Süleyman Demirel University Journal of Natural and Applied Sciences is indexed in Chemical Abstracts Service, EBSCO Discovery Service, Google Scholar, WorldCat and Zentralblatt MATH.

Trend on High-speed Power Line Communication Technology

Science.gov (United States)

Ogawa, Osamu

High-speed power line communication (PLC) is useful technology to easily build the communication networks, because construction of new infrastructure is not necessary. In Europe and America, PLC has been used for broadband networks since the beginning of 21th century. In Japan, high-speed PLC was deregulated only indoor usage in 2006. Afterward it has been widely used for home area network, LAN in hotels and school buildings and so on. And recently, PLC is greatly concerned as communication technology for smart grid network. In this paper, the author surveys the high-speed PLC technology and its current status.

77 FR 63905 - Self-Regulatory Organizations; ICE Clear Credit LLC; Notice of Filing of Proposed Rule Change To...

Science.gov (United States)

2012-10-17

... company; COMPAGNIE DE SAINT-GOBAIN; Deutsche Telekom AG; FRANCE TELECOM; GAS NATURAL SDG, S.A. GDF SUEZ...; COMPASS GROUP PLC; EXPERIAN FINANCE PLC; GROUPE AUCHAN; J SAINSBURY plc; Koninklijke Ahold N.V.; MARKS AND...

Reproductive factors, menopausal hormone therapies and primary liver cancer risk: a systematic review and dose-response meta-analysis of observational studies.

Science.gov (United States)

Zhong, Guo-Chao; Liu, Yan; Chen, Nan; Hao, Fa-Bao; Wang, Kang; Cheng, Jia-Hao; Gong, Jian-Ping; Ding, Xiong

2016-12-01

A striking gender disparity in the incidence and outcome of primary liver cancer (PLC) has been well recognized. Mounting evidence from basic research suggests that hormonal factors may be involved in the gender disparity of PLC. Whether hormonal exposures in human subjects are associated with PLC risk is largely unknown. Whether reproductive factors and use of menopausal hormone therapies (MHTs) in women are associated with PLC risk remains controversial. We conducted this study to clarify this issue. PubMed and EMBASE were searched to July, 2016 for studies published in English or Chinese. Observational studies (cohort, nested case-control and case-control) that provided risk estimates of reproductive factors, MHTs and PLC risk were eligible. The quality of included studies was determined based on the Newcastle-Ottawa quality assessment scale. Summary risk ratios (RRs) were calculated using a random-effects model. Dose-response analysis was conducted where possible. Fifteen peer-reviewed studies, involving 1795 PLC cases and 2 256 686 women, were included. Overall meta-analyses on parity and PLC risk did not find any significant associations; however, when restricting to studies with PLC cases ≥100, increasing parity was found to be significantly associated with a decreased risk of PLC [RR for the highest versus lowest parity 0.67, 95% CI 0.52, 0.88; RR for parous versus nulliparous 0.71, 95% CI 0.53, 0.94; RR per one live birth increase 0.93, 95% CI 0.88, 0.99]. A J-shaped relationship between parity and PLC risk was identified (P non-linearity  Parity is associated with PLC risk in a J-shaped dose-response pattern. Late age at menarche and ever use of MHT are associated with a reduced risk of PLC, whereas there is no association of ever use of ET and EPT, age at first birth, or spontaneous and induced abortion with PLC risk. Compared to women with no history of oophorectomy, those with a history of oophorectomy are at an increased risk of PLC. Our findings

Demonstration of Supervisory Control and Data Acquisition (SCADA) Virtualization Capability in the US Army Research Laboratory (ARL)/Sustaining Base Network Assurance Branch (SBNAB) US Army Cyber Analytics Laboratory (ACAL) SCADA Hardware Testbed

Science.gov (United States)

2015-05-01

application ,1 while the simulated PLC software is the open source ModbusPal Java application . When queried using the Modbus TCP protocol, ModbusPal reports...and programmable logic controller ( PLC ) components. The HMI and PLC components were instantiated with software and installed in multiple virtual...creating and capturing HMI– PLC network traffic over a 24-h period in the virtualized network and inspect the packets for errors.  Test the

Pathological Laughter and Crying and Psychiatric Comorbidity After Traumatic Brain Injury.

Science.gov (United States)

Roy, Durga; McCann, Una; Han, Dingfen; Rao, Vani

2015-01-01

There are limited data regarding the incidence of pathological laughter and crying (PLC) after traumatic brain injury (TBI). This study aimed to identify the occurrence of PLC in the first year after TBI and to determine whether there is a relationship between PLC and other clinical features or demographics. Subjects who sustained a first-time TBI were recruited from acute trauma units and were assessed at 3, 6, and 12 months after TBI. Rates of PLC at 3, 6, and 12 months after TBI were 21.4%, 17.5%, and 15.5%, respectively. Patients with PLC had higher percentages of psychiatric diagnoses, including personality changes, depressive disorders, and mood disorders secondary to a general medical condition, as well as higher rates of posttraumatic stress disorder. Univariate logistic and linear regression analyses indicated a significant association between PLC and scores on the Clinical Anxiety Scale 3 months after TBI and on the Hamilton Depression Rating Scale 12 months after TBI. Individuals who have PLC during the first year after TBI are more likely to have any psychiatric diagnosis as well as higher rates of mood and anxiety symptoms. In addition, PLC in the early TBI period may serve as a predictor of depression and anxiety symptoms at 12 months after TBI.

Pleural lavage cytology as an independent prognostic factor in non-small-cell lung cancer patients with stage I disease and adenocarcinoma.

Science.gov (United States)

Hokka, Daisuke; Uchino, Kazuya; Tane, Kenta; Ogawa, Hiroyuki; Tane, Sinya; Tanaka, Yugo; Tauchi, Shunsuke; Nishio, Wataru; Yoshimura, Masahiro; Maniwa, Yoshimasa

2015-01-01

We previously reported that cancer cells may be detected through pleural lavage cytology (PLC). In this study, we sought to re-examine the prognostic significance of the PLC status based on an extended dataset with an additional follow-up period. Pleural lavage following thoracotomy was cytologically examined in 1,317 consecutive patients who were diagnosed with NSCLC between 1987 and 2004 at the Thoracic Surgery Units of Kobe University Graduate School of Medicine and Hyogo Cancer Center. Among the investigated patients, 46 exhibited positive cytological findings. The prognosis of these patients was significantly worse compared to that of patients without positive PLC. Of the 844 pathological stage I patients, 18 had a positive PLC status and their prognosis was significantly worse compared to that of patients with stage I disease without positive PLC. In conclusion, positive PLC findings were associated with a poor prognosis and this finding was significant for patients with stage I disease. These results suggest the need for PLC status evaluation during staging and treatment planning in patients with NSCLC.

Clinicopathological characteristics and survival outcomes in pleomorphic lobular breast carcinoma of the breast: a SEER population-based study.

Science.gov (United States)

Yang, Li-Peng; Sun, He-Fen; Zhao, Yang; Chen, Meng-Ting; Zhang, Nong; Jin, Wei

2017-12-01

The purpose of this study was to explore the clinicopathological features and survival outcome of pleomorphic lobular carcinoma (PLC) of breast, we identified 131 PLC patients and 460,109 invasive ductal carcinoma (IDC) patients in the Surveillance, Epidemiology, and End Result (SEER) database. PLCs presented with increased lymph node involvement, older age, higher AJCC stage and grade, and lower median survival months (PLC 84 ± 51.03 vs. IDC 105.2 ± 64.39 P PLC patients were more inclined to be treated with mastectomy. In univariate analysis, PLC patients showed a worse disease-specific survival (DSS) than that of IDC patients (hazard ratio = 0.691, 95% confidence interval 0.534-0.893, P PLC groups (P = 0.615). This result may be due to PLCs presenting higher tumor stage, higher tumor grade, and higher rate of LN metastasis than IDCs. Our conclusion is that PLC and IDC have many different characteristics, but there is not enough difference on the DSS. © 2017 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

A PC-PCL-based control system for the high-voltage pulsed-power operation of the Intense Diagnostic Neutral Beam (IDNB) Experiment

International Nuclear Information System (INIS)

Gribble, R.

1993-06-01

A stand-alone, semiautomated control system for the high-voltage pulsed-power energy sources on the Intense Diagnostic Neutral Beam Experiment at Los Alamos National Laboratory using personal computer (PC) and programmable logic controller (PLC) technology has been developed and implemented. The control system, consisting of a PC with the graphic operator interface, the network connecting the PC to the PLC, the PLC, the PLC I/O modules, fiber-optic interfaces and software, is described

Methods in Logic Based Control

DEFF Research Database (Denmark)

Christensen, Georg Kronborg

1999-01-01

Desing and theory of Logic Based Control systems.Boolean Algebra, Karnaugh Map, Quine McClusky's algorithm. Sequential control design. Logic Based Control Method, Cascade Control Method. Implementation techniques: relay, pneumatic, TTL/CMOS,PAL and PLC- and Soft_PLC implementation. PLC...

Use of advanced programmable controllers in LCTF

International Nuclear Information System (INIS)

Thompson, D.H.; Baylor, L.R.; Fletcher, W.M.; Patek, D.R.

1983-01-01

The present generation of industrial programmable logic controllers (PLCs) offers a variety of features that are ideal for use in largescale fusion experiments. Among these features are computational capabilities, communication interfaces, ease of programming, and, most importantly, high reliability. In the Large Coil Test Facility (LCTF), three PLCs control all of the major systems, including liquid helium, liquid nitrogen, vacuum, vapor-cooled leads, coil protection, power supplies, and personnel protection. The PLC control system for LCTF is divided into three functional blocks. The first is the vacuum PLC that monitors all tank functions and has a graphic display panel on the Master Control Console (MCC). A second PLC monitors all time-critical coil protection functions and has the capability to initiate a dump of the stored energy in the coils when a setpoint is exceeded in coil or vapor-cooled lead monitoring instruments. The third PLC, which is designated the facility PLC, monitors and controls the bulk of the LCTF facility systems and serves as a redundant coil protection PLC. The facility PLC is a Modicon 584, level-IV 32K word machine with enhanced software features that include Proportional Integral Derivative (PID) control loops, block functions, and table lookups. A low-cost, color computer graphics terminal located in the MCC is attached to the Modbus link of the facility PLC and has the capability of displaying alarms and operating valves. A hard-copy terminal driven directly by the PLC logs all alarms and valve operations. Twenty-two process loops, including vapor-cooled lead flow, are controlled via the PID functions. The PLC-based control system in LCTF is the result of a philosophy that reliable control needs to be separate from data acquisition. This has resulted in a control system with maximum flexibility constructed entirely of off-the-shelf components and minimal software effort

Use of advanced programmable controllers in LCTF

International Nuclear Information System (INIS)

Thompson, D.H.; Baylor, L.R.; Patek, D.R.; Fletcher, W.M.

1983-01-01

The present generation of industrial programmable logic controllers (PLCs) offers a variety of features that are ideal for use in large-scale fusion experiments. Among these features are computational capabilities, communication interfaces, ease of programming, and, most importantly, high reliability. In the Large Coil Test Facility (LCTF), three PLCs control all of the major systems, including liquid helium, liquid nitrogen, vacuum, vapor-cooled leads, coil protection, power supplies, and personnel protection. The PLC control system for LCTF is divided into three functional blocks. The first is the vacuum PLC that monitors all tank functions and has a graphic display panel on the Master Control Console (MCC). A second PLC monitors all time-critical coil protection functions and has the capability to initiate a dump of the stored energy in the coils when a setpoint is exceeded in coil or vapor-cooled lead monitoring instruments. The third PLC, which is designated the facility PLC, monitors and controls the bulk of the LCTF facility systems and serves as a redundant coil protection PLC. The facility PLC is a Modicon 584, level-IV 32K word machine with enhanced software features that include Proportional Integral Derivative (PID) control loops, block functions, and table lookups. A low-cost, color computer graphics terminal located in the MCC is attached to the Modbus link of the facility PLC and has the capability of displaying alarms and operating valves. A hard-copy terminal driven directly by the PLC logs all alarms and valve operations. Twenty-two process loops, including vapor-cooled lead flow, are controlled via the PID functions

Platelet activation by bacterial phospholipase C involves phosphoinositide turnover and phosphorylation of 47,000 dalton but not 20,000 dalton protein

International Nuclear Information System (INIS)

Huzoor-Akbar; Anwer, K.

1986-01-01

This study was conducted to examine the role of phosphoinositides (PIns) and phosphorylation of 47,000 dalton (P47) and 20,000 dalton (P20) proteins in platelet activation by bacterial phospholipase C (PLC). PLC induced serotonin secretion (SS) and platelet aggregation (PA) in a concentration dependent manner. PLC (0.02 U/ml) caused phosphorylation of P47 in a time dependent manner (27% at 0.5 min to 378% at 7 min). PLC did not induce more than 15% phosphorylation of P20 by 7 min. Aspirin (500 μM) blocked phosphorylation of P20 but did not inhibit SS, PA or phosphorylation of P47. PLC (0.04 U/ml) decreased radioactivity (cpm) in 32 P labeled phosphatidylinositol (PI), PI-4,5-bis-PO4 (PIP2) and PI-4-PO4 (PIP) by 20%, 12% and 7.5% respectively at 15 sec. The level of PI but not that of PIP2 returned to base line in 3 min. PIP level increased above control values within one min. PLC increased phosphatidic acid level (75% at 0.5 min. to 1545% at 3 min). In other experiments PLC produced diacylglycerol (DAG) in a time and concentration dependent manner. However, no DAG was detectable in the first 60 sec. These data suggest that: (a) PIns turnover and phosphorylation of P47 but not that of P20 is involved in platelet activation by PLC; and (b) DAG production from outer membrane phospholipids is not a prerequisite for platelet activation by PLC

New insights into molecular diagnostic pathology of primary liver cancer: Advances and challenges.

Science.gov (United States)

Cong, Wen-Ming; Wu, Meng-Chao

2015-11-01

Primary liver cancer (PLC) is one of the most common malignancies worldwide with increasing incidence and accounts for the third leading cause of cancer-related mortality. Traditional morphopathology primarily emphasizes qualitative diagnosis of PLC, which is not sufficient to resolve the major concern of increasing the long-term treatment efficacy of PLC in clinical management for the modern era. Since the beginning of the 21st century, molecular pathology has played an active role in the investigation of the evaluation of the metastatic potential of PLC, detection of drug targets, prediction of recurrence risks, analysis of clonal origins, evaluation of the malignancy trend of precancerous lesions, and determination of clinical prognosis. As a result, many new progresses have been obtained, and new strategies of molecular-pathological diagnosis have been formed. Moreover, the new types of pathobiological diagnosis indicator systems for PLC have been preliminarily established. These achievements provide valuable molecular pathology-based guide for clinical formulation of individualized therapy programs for PLC. This review article briefly summarizes some relevant progresses of molecular-pathological diagnosis of PLC from the perspective of clinical translational application other than basic experimental studies. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

The Programmable Logic Controller and its application in nuclear reactor systems

International Nuclear Information System (INIS)

Palomar, J.; Wyman, R.

1993-09-01

This document provides recommendations to guide reviewers in the application of Programmable Logic Controllers (PLCS) to the control, monitoring and protection of nuclear reactors. The first topics addressed are system-level design issues, specifically including safety. The document then discusses concerns about the PLC manufacturing organization and the protection system engineering organization. Supplementing this document are two appendices. Appendix A summarizes PLC characteristics. Specifically addressed are those characteristics that make the PLC more suitable for emergency shutdown systems than other electrical/electronic-based systems, as well as characteristics that improve reliability of a system. Also covered are PLC characteristics that may create an unsafe operating environment. Appendix B provides an overview of the use of programmable logic controllers in emergency shutdown systems. The intent is to familiarize the reader with the design, development, test, and maintenance phases of applying a PLC to an ESD system. Each phase is described in detail and information pertinent to the application of a PLC is pointed out

The Programmable Logic Controller and its application in nuclear reactor systems

Energy Technology Data Exchange (ETDEWEB)

Palomar, J.; Wyman, R. [Lawrence Livermore National Lab., CA (United States)

1993-09-01

This document provides recommendations to guide reviewers in the application of Programmable Logic Controllers (PLCS) to the control, monitoring and protection of nuclear reactors. The first topics addressed are system-level design issues, specifically including safety. The document then discusses concerns about the PLC manufacturing organization and the protection system engineering organization. Supplementing this document are two appendices. Appendix A summarizes PLC characteristics. Specifically addressed are those characteristics that make the PLC more suitable for emergency shutdown systems than other electrical/electronic-based systems, as well as characteristics that improve reliability of a system. Also covered are PLC characteristics that may create an unsafe operating environment. Appendix B provides an overview of the use of programmable logic controllers in emergency shutdown systems. The intent is to familiarize the reader with the design, development, test, and maintenance phases of applying a PLC to an ESD system. Each phase is described in detail and information pertinent to the application of a PLC is pointed out.

Time trends in incidence and prognosis of primary liver cancer and liver metastases of unknown origin in a Danish region, 1985-2004

DEFF Research Database (Denmark)

Erichsen, Rune; Jepsen, Peter; Jacobsen, Jacob

2008-01-01

OBJECTIVES: Changes, over the last 20 years, in the diagnostic procedures and treatment of primary liver cancer (PLC) and liver metastases of unknown origin (LMUO) may have affected the clinical course of both cancers. Few longitudinal studies examined this issue. In a population-based setting, we...... studied changes in the incidence and prognosis of PLC and LMUO over time. METHODS: Between 1985 and 2004, we identified 2675 patients with PLC and LMUO in three Danish counties, with a population of 1.4 million. We computed the standardized incidence rate (SIR), ratio of PLC to LMUO diagnoses, median...... survival, and estimated mortality rate ratio adjusted for age, sex, and comorbidity. RESULTS: The SIR of PLC increased from 3.2 in 1985 to 5.0 in 2003, and the SIR of LMUO increased from 3.7 to 6.4. No increase was noted in the PLC-to-LMUO ratio over time (P=0.1 for trend). From 1985 to 2004, the median...

Overactivation of phospholipase C-gamma1 renders platelet-derived growth factor beta-receptor-expressing cells independent of the phosphatidylinositol 3-kinase pathway for chemotaxis

DEFF Research Database (Denmark)

Rönnstrand, L; Siegbahn, A; Rorsman, C

1999-01-01

., Siegbahn, A. , Rorsman, C., Engström, U., Wernstedt, C., Heldin, C.-H., and Rönnstrand, L. (1996) EMBO J. 15, 5299-5313). Here we show that the increased chemotaxis correlates with increased activation of phospholipase C-gamma1 (PLC-gamma1), measured as inositol-1,4, 5-trisphosphate release. By two......-dimensional phosphopeptide mapping, the increase in phosphorylation of PLC-gamma1 was shown not to be selective for any site, rather a general increase in phosphorylation of PLC-gamma1 was seen. Specific inhibitors of protein kinase C, bisindolylmaleimide (GF109203X), and phosphatidylinositol 3-kinase (PI3-kinase), LY294002......, did not affect the activation of PLC-gamma1. To assess whether increased activation of PLC-gamma1 is the cause of the hyperchemotactic behavior of the Y934F mutant cell line, we constructed cell lines expressing either wild-type or a catalytically compromised version of PLC-gamma1 under a tetracycline...

Posterolateral Corner Injury Associated with a Schatzker Type 2 Tibial Plateau Fracture

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Boris A. Zelle

2015-01-01

Full Text Available Isolated posterolateral corner (PLC injuries are rarely seen with tibial plateau fractures and can be missed during the initial assessment. The objective of this paper is to present a case of a Schatzker type 2 tibial plateau fracture with associated isolated PLC injury and give a discussion on physical exam, diagnostic studies, and treatment options. A twenty-five-year-old female sustained a concomitant Schatzker type 2 fracture and PLC injury. Magnetic Resonance Imaging showed an isolated PLC disruption. Open reduction-internal fixation was performed with subsequent PLC repair. At sixteen months postoperatively, the patient had full range of motion and strength of her knee and no signs of laxity. This case emphasizes the importance of physical exam and appropriate imaging modalities in order to diagnose and treat this significant injury in a prompt fashion. In this case, surgical fracture fixation and subsequent repair of the PLC provided a good clinical outcome.

Novel roles for phospholipase C in plant stress signalling and development

NARCIS (Netherlands)

Zhang, Q.

2017-01-01

For many years, efforts have been made to explore PLC signaling in plants. Compared to the classical PLC signaling pathway, a different picture is emerging for plants. Several roles for PLC in plant development and stress responses have been claimed but genetic evidence for this is mostly missing.

PERBANDINGAN JENIS MEDIA TANAM TANAH ENTISOL YANG TERPAPAR SATU KALI DAN DUA KALI OLEH LIMBAH CAIR NANAS TERHADAP PERTUMBUHAN SELEDRI (Apium graveolens L.) SEBAGAI SUMBER BELAJAR BIOLOGI

OpenAIRE

Irma Yunita Sari Kartika Sari

2015-01-01

Abstract. This research to know effect of Pineapple Liquid Compost (PLC) in long term the growth of celery. This research conducted at the village in Yosodadi 21 Polos, East Metro, Metro City since 7 December until 28 February 2015. This research is experimental research, the design used the Complete Random Design (CRD). This research used entisol soil that was exposed by PLC once and entisol soil that was exposed by PLC twice. The treatment was done to them both by giving PLC about 200ml in ...

Use of advanced programmable logic controllers to monitor and control the Elmo Bumpy Torus-proof-of-principle device

International Nuclear Information System (INIS)

Boyd, B.A.

1983-01-01

The Elmo Bumpy Torus - Proof-of-Principle (EBT-P) device is designed with an instrumentation and control system based upon the use of an advanced Programmable Logic Controller (PLC). The modern PLC incorporates many advanced programming features not available in earlier PLC's intended for application to conventional relay logic replacement. The additional power and flexibility of these modern PLC's is especially applicable to an experimental device such as EBT-P which is made up of several complex interrelated subsystems whose operational characteristics will be evolving throughout the lifetime of the device. The rationale for the selection of advanced PLC's for EBT-P and the approach taken to design of the software developed to control EBT-P are the topics addressed in this paper

Difference in F-18 FDG Uptake According to the Patterns of CT-Based Diagnosed Pulmonary Lymphangitic Carcinomatosis in Patients with Lung Cancer

International Nuclear Information System (INIS)

Jun, Sung Min; Kim, Yong Ki; Kim, In Ju; Kim, Seong Jang; Nam, Hyun Yeol; Kim, Bum Soo

2008-01-01

Our purpose was to evaluate F-18 FDG uptake in pulmonary lymphangitic carcinomatosis (PLC) according to CT findings and histology of lung cancer. Thirty-three lung cancer patients with PLC were enrolled in this retrospective study. All the patients had a CT-based diagnosis of PLC. Chest CT findings of PLC were classified on the basis of involvement of axial interstitium. We categorized the involvement of axial interstitium as group 1, and the involvement of peripheral interstitium only as group 2. Visual and semiquantitative analyses by F-18 FDG PET/CT were performed in the PLC lesions. At first, we analyzed the F-18 FDG uptake in the PLC by visual assessment. If abnormal uptake was seen in the PLC, we drew regions of interest in the PLC lesions to obtain the maximum SUVs (maxSUVs). Of the 33 patients, 22 had abnormal F-18 FDG uptake in the visual assessment. There was no significant difference in the frequency of abnormal F-18 FDG uptake between group 1 and group 2 (p=0.17), although the frequency of group 1 tended to be higher than group 2 (15/19 (78.9%) in group 1, 7/14 (50.0%) in group 2). However, group 1 had a higher maxSUV than group 2 (p<0.01, group 1: 2.9±1.4, group 2: 1.5±0.6). There was no significant difference in the frequency of abnormal F-18 FDG uptake and maxSUV among the histology of the lung cancers. The involvement of axial interstitium in the PLC by lung cancer has a higher maxSUV than the involvement of only peripheral interstitium

Propionyl-L-carnitine improves endothelial function, microcirculation and pain management in critical limb ischemia.

Science.gov (United States)

De Marchi, S; Zecchetto, S; Rigoni, A; Prior, M; Fondrieschi, L; Scuro, A; Rulfo, F; Arosio, E

2012-10-01

Chronic critical limb ischemia (CLI) is a severe condition of hypo-perfusion of lower limbs, which is associated with inflammation and a pro-coagulative state. It is a disease at high risk of amputation and cardiovascular death. Propionyl-L-carnitine (PLC) is efficacious in improving pain free walking distance in peripheral arterial disease with claudication; it also exerts favorable effects on the arterial wall and on endothelial function. The purpose of this study was to evaluate the effects of PLC on microcirculation, endothelial function and pain relief in patients affected by CLI not suitable for surgical intervention. We enrolled 48 patients with CLI. Patients were randomized into two groups: the first group was treated with PLC, the second was treated with saline solution. All of them underwent the following tests: laser Doppler flowmetry at the forefoot at rest and after ischemia, trans cutaneous oxygen partial pressure and carbon dioxide partial pressure at the forefoot at rest and after ischemia, endothelium dependent dilation of the brachial artery. All tests were repeated after treatments. Pain was assessed by visual analog pain scale. Endothelium dependent dilation increased after PLC (9.5 ± 3.2 vs 4.9 ± 1.4 %; p < 0.05). Post-ischemic peak flow with laser-Doppler flow increased after PLC. TcPO2 increased, while TcPCO2 decreased after PLC; CO2 production decreased after PLC. VAS showed a significant reduction in pain perception after active treatment. In CLI patients, PLC can improve microcirculation (post ischemic hyperemia, TcPO2 and TcPCO2 production). PLC also enhances endothelium dependent dilation and reduces analgesic consumption and pain perception.

Dynamics of major histocompatibility complex class I association with the human peptide-loading complex.

Science.gov (United States)

Panter, Michaela S; Jain, Ankur; Leonhardt, Ralf M; Ha, Taekjip; Cresswell, Peter

2012-09-07

Although the human peptide-loading complex (PLC) is required for optimal major histocompatibility complex class I (MHC I) antigen presentation, its composition is still incompletely understood. The ratio of the transporter associated with antigen processing (TAP) and MHC I to tapasin, which is responsible for MHC I recruitment and peptide binding optimization, is particularly critical for modeling of the PLC. Here, we characterized the stoichiometry of the human PLC using both biophysical and biochemical approaches. By means of single-molecule pulldown (SiMPull), we determined a TAP/tapasin ratio of 1:2, consistent with previous studies of insect-cell microsomes, rat-human chimeric cells, and HeLa cells expressing truncated TAP subunits. We also report that the tapasin/MHC I ratio varies, with the PLC population comprising both 2:1 and 2:2 complexes, based on mutational and co-precipitation studies. The MHC I-saturated PLC may be particularly prevalent among peptide-selective alleles, such as HLA-C4. Additionally, MHC I association with the PLC increases when its peptide supply is reduced by inhibiting the proteasome or by blocking TAP-mediated peptide transport using viral inhibitors. Taken together, our results indicate that the composition of the human PLC varies under normal conditions and dynamically adapts to alterations in peptide supply that may arise during viral infection. These findings improve our understanding of the quality control of MHC I peptide loading and may aid the structural and functional modeling of the human PLC.

Short Stat5-interacting peptide derived from phospholipase C-β3 inhibits hematopoietic cell proliferation and myeloid differentiation.

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Hiroki Yasudo

Full Text Available Constitutive activation of the transcription factor Stat5 in hematopoietic stem/progenitor cells leads to various hematopoietic malignancies including myeloproliferative neoplasm (MPN. Our recent study found that phospholipase C (PLC-β3 is a novel tumor suppressor involved in MPN, lymphoma and other tumors. Stat5 activity is negatively regulated by the SH2 domain-containing protein phosphatase SHP-1 in a PLC-β3-dependent manner. PLC-β3 can form the multimolecular SPS complex together with SHP-1 and Stat5. The close physical proximity of SHP-1 and Stat5 brought about by interacting with the C-terminal segment of PLC-β3 (PLC-β3-CT accelerates SHP-1-mediated dephosphorylation of Stat5. Here we identify the minimal sequences within PLC-β3-CT required for its tumor suppressor function. Two of the three Stat5-binding noncontiguous regions, one of which also binds SHP-1, substantially inhibited in vitro proliferation of Ba/F3 cells. Surprisingly, an 11-residue Stat5-binding peptide (residues 988-998 suppressed Stat5 activity in Ba/F3 cells and in vivo proliferation and myeloid differentiation of hematopoietic stem/progenitor cells. Therefore, this study further defines PLC-β3-CT as the Stat5- and SHP-1-binding domain by identifying minimal functional sequences of PLC-β3 for its tumor suppressor function and implies their potential utility in the control of hematopoietic malignancies.

Performance Testing Methodology for Safety-Critical Programmable Logic Controller

International Nuclear Information System (INIS)

Kim, Chang Ho; Oh, Do Young; Kim, Ji Hyeon; Kim, Sung Ho; Sohn, Se Do

2009-01-01

The Programmable Logic Controller (PLC) for use in Nuclear Power Plant safety-related applications is being developed and tested first time in Korea. This safety-related PLC is being developed with requirements of regulatory guideline and industry standards for safety system. To test that the quality of the developed PLC is sufficient to be used in safety critical system, document review and various product testings were performed over the development documents for S/W, H/W, and V/V. This paper provides the performance testing methodology and its effectiveness for PLC platform conducted by KOPEC

Intrinsic Pleckstrin Homology (PH) Domain Motion in Phospholipase C-β Exposes a Gβγ Protein Binding Site*

OpenAIRE

Kadamur, Ganesh; Ross, Elliott M.

2016-01-01

Mammalian phospholipase C-β (PLC-β) isoforms are stimulated by heterotrimeric G protein subunits and members of the Rho GTPase family of small G proteins. Although recent structural studies showed how Gαq and Rac1 bind PLC-β, there is a lack of consensus regarding the Gβγ binding site in PLC-β. Using FRET between cerulean fluorescent protein-labeled Gβγ and the Alexa Fluor 594-labeled PLC-β pleckstrin homology (PH) domain, we demonstrate that the PH domain is the minimal Gβγ binding region in...

MANUAL LOGIC CONTROLLER (MLC)

OpenAIRE

Claude Ziad Bayeh

2015-01-01

The “Manual Logic Controller” also called MLC, is an electronic circuit invented and designed by the author in 2008, in order to replace the well known PLC (Programmable Logic Controller) in many applications for its advantages and its low cost of fabrication. The function of the MLC is somewhat similar to the well known PLC, but instead of doing it by inserting a written program into the PLC using a computer or specific software inside the PLC, it will be manually programmed in a manner to h...

Glucose and carbachol activate phospholipase C in digitonin-permeabilized islets

International Nuclear Information System (INIS)

Wolf, B.A.; Florholmen, J.; Turk, J.; McDaniel, M.L.

1987-01-01

Stimulation of intact islets with D-glucose, the major insulin secretagogue, or with carbachol, a muscarinic agonist, results in the accumulation of inositoltrisphosphate (IP 3 ) suggesting that activation of phospholipase C (PLC) has a major role in stimulus-secretion coupling. Carbachol activation of PLC is an example of receptor-mediated activation in islets, whereas, the mechanism of glucose activation of PLC is controversial since a glucose receptor has not been identified. They have measured PLC activity in digitonin-permeabilized islets. Islets were labeled with 3 H-inositol, permeabilized and IP 3 accumulation measured by HPLC. Carbachol, in the presence of ATP, GTP and 1 μM free Ca 2+ released two-fold more Ins 1,3,4-P 3 than control in a time-dependent manner. Glucose, under the same conditions also significantly released more Ins 1,3,4-P 3 than control. This effect was not due to metabolism of glucose nor to an effect on the IP 3 -phosphomonoesterase. Preliminary Ca 2+ -dependency studies indicate that PLC is not activated by Ca 2+ in the submicromolar range. In conclusion, these studies show that Ca 2+ does not activate PLC, and furthermore, that D-glucose may be recognized directly by PLC

Stalling autophagy: a new function for Listeria phospholipases

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Ivan Tattoli

2014-01-01

Full Text Available Listeria monocytogenes is a Gram-positive bacterial pathogen that induces its own uptake in non-phagocytic cells. Following invasion, Listeria escapes from the entry vacuole through the secretion of a pore-forming toxin, listeriolysin O (LLO that acts to damage and disrupt the vacuole membrane. Listeria then replicates in the cytosol and is able to spread from cell-to-cell using actin-based motility. In addition to LLO, Listeria produces two phospholipase toxins, a phosphatidylinositol-specific phospholipase C (PI-PLC, encoded by plcB and a broad-range phospholipase C (PC-PLC, encoded by plcA, which contribute to bacterial virulence. It has long been recognized that secretion of PI- and PC-PLC enables the disruption of the double membrane vacuole during cell-to-cell spread, and those phospholipases have also been shown to augment LLO-dependent escape from the entry endosome. However, a specific role for Listeria phospholipases during the cytosolic stage of infection has not been previously reported. In a recent study, we demonstrated that Listeria PI-PLC and PC-PLC contribute to the bacterial escape from autophagy through a mechanism that involves direct inhibition of the autophagic flux in the infected cells [Tattoli et al. EMBO J (2013, 32, 3066-3078].

Primary liver carcinoma and liver cirrhosis in atomic bomb survivors, Hiroshima and Nagasaki, 1961-75, with special reference to HBs antigen

International Nuclear Information System (INIS)

Asano, Masahide; Kato, Hiroo; Yoshimoto, Keiko; Seyama, Shinichi; Itakura, Hideyo.

1982-03-01

During 1961-75, 128 cases of primary liver carcinoma (PLC) in the RERF Life Span Study extended sample and 301 cases of liver cirrhosis in the RERF Pathology Study sample were observed. All cases were assessed for hepatitis B surface antigen (HB sub(s) Ag) using orcein and aldehyde fuchsin staining. The incidence of PLC was 2.0 times higher in Nagasaki than in Hiroshima which was statistically significant, but the prevalence of liver cirrhosis showed hardly any difference between the two cities. Meaningful findings that may possibly explain the higher incidence of PLC in Nagasaki were that the presence of HB sub(s) Ag in the liver of patients without overt liver disease was 2.3 times higher in Nagasaki than in Hiroshima, and the prevalence of liver cirrhosis associated with PLC, especially that of posthepatitic cirrhosis with PLC, was almost 2.0 times higher in Nagasaki than in Hiroshima. In both cities a suggestive relationship of radiation dose with the prevalence of liver cirrhosis was noted but not with PLC. We believe that the higher incidence of PLC in Nagasaki is attributable to HB virus infection, though other factors, such as immunological competence affected by radiation, cannot be excluded. (author)

IPv6 (Internet Protocol version 6) heterogeneous networking infrastructure for energy efficient building

International Nuclear Information System (INIS)

Ben Saad, Leila; Chauvenet, Cedric; Tourancheau, Bernard

2012-01-01

In the context of increasing developments of home, building and city automation, Power Line Communication (PLC) networking is called for unprecedented usage, especially for energy efficiency improvement. Our view of the future building networking infrastructure places PLC as the central point. We point that while Wireless Sensor Network (WSN) is necessary in the sensor and actuator networking infrastructure, PLC is mandatory for the smart-grid metering and command infrastructure. PLC will also serve the sensor/actuator infrastructure when the energy requirement of the probing system itself cannot be fulfilled by autonomous battery and harvesting based nodes. PLC may also provide the numerous bridges necessary to sustain a long lifetime (years) with the battery based WSN part of the infrastructure. This new role of PLC networking will be possible only if the interoperability between all media and technology is made possible. Thanks to the converging design of Internet Protocol version 6 (IPv6) networking layers, we show that such a full inter-interoperability is already possible even in very tiny constrained networking devices. Moreover, the low power PLC technology used in our experiments will be able to provide this smart grid monitoring without impacting noticeably the overall energy balance of the monitored system.