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Sample records for dysprosium 145

  1. Dysprosium magneto-optical traps

    CERN Document Server

    Youn, Seo Ho; Ray, Ushnish; Lev, Benjamin L

    2010-01-01

    Magneto-optical traps (MOTs) of highly magnetic lanthanides open the door to explorations of novel phases of strongly correlated matter such as lattice supersolids and quantum liquid crystals. We recently reported the first MOTs of the five high abundance isotopes of the most magnetic atom, dysprosium. Described here are details of the experimental technique employed for repumper-free Dy MOTs containing up to half a billion atoms. Extensive characterization of the MOTs' properties---population, temperature, loading, metastable decay dynamics, trap dynamics---is provided.

  2. On polymorphism of dysprosium trichloride

    Energy Technology Data Exchange (ETDEWEB)

    Zakiryanova, Irina D.; Khokhlov, Vladimir A.; Salyulev, Alexander B.; Korzun, Iraida V. [RAS Ural Branch, Ekaterinburg (Russian Federation). Institute of High-Temperature Electrochemistry

    2015-07-01

    For the first time, the structure of crystalline DyCl{sub 3} over a wide temperature range from room temperature to melting point was studied by Raman spectroscopy. The phonon modes (cm{sup -1}) of dysprosium trichloride (monoclinic crystal lattice of AlCl{sub 3} type, Z = 4, CN = 6) at room temperature are 257 (A{sub 1g}), 201 (E{sub g}), 112 (E{sub g}), 88 (A{sub 1g}), and 63 (E{sub g}). The monoclinic structure of the crystalline DyCl{sub 3} C{sub 2h}{sup 3} symmetry was found to remain constant over the studied temperature range. No polymorphic transformation in the solid state was detected. Gravimetry, calorimetry, and mass spectrometry have been used in addition to support the conclusions made on the basis of Raman spectroscopic data.

  3. Resonance ionization spectroscopy in dysprosium

    Energy Technology Data Exchange (ETDEWEB)

    Studer, D., E-mail: dstuder@uni-mainz.de; Dyrauf, P.; Naubereit, P.; Heinke, R.; Wendt, K. [Johannes Gutenberg-Universität Mainz, Institut für Physik (Germany)

    2017-11-15

    We report on resonance ionization spectroscopy (RIS) of high-lying energy levels in dysprosium. We developed efficient excitation schemes and re-determined the first ionization potential (IP) via analysis of Rydberg convergences. For this purpose both two- and three-step excitation ladders were investigated. An overall ionization efficiency of 25(4) % could be demonstrated in the RISIKO mass separator of Mainz University, using a three-step resonance ionization scheme. Moreover, an extensive analysis of the even-parity 6sns- and 6snd-Rydberg-series convergences, measured via two-step excitation was performed. To account for strong perturbations in the observed s-series, the approach of multichannel quantum defect theory (MQDT) was applied. Considering all individual series limits we extracted an IP-value of 47901.76(5) cm{sup −1}, which agrees with the current literature value of 47901.7(6) cm{sup −1}, but is one order of magnitude more precise.

  4. The dysprosium-tin phase diagram

    Energy Technology Data Exchange (ETDEWEB)

    Eremenko, V.N.; Bulanova, M.V.; Martsenjuk, P.S. (I.N. Frantsevich Inst. for Problems of Materials Science, Kiev (Ukraine))

    1992-12-07

    The dysprosium-tin phase diagram was established by means of differential thermal, X-ray and microscopic analyses of 22 alloys. Seven intermetallic compounds were found to exist in the system. Dy[sub 5]Sn[sub 3] melts congruently at 1870 degC, and undergoes a polymorphous transformation at 1823 [+-] 6 degC. The intermetallics Dy[sub 5]Sn[sub 4], Dy[sub 11]Sn[sub 10], DySn, Dy[sub 4]Sn[sub 5], DySn[sub 2], DySn[sub 3] are formed peritectically at 1712 [+-]11, 1605 [+-]12, 1208 [+-]3, 1166 [+-]7, 1138 [+-]3 and 747 [+-]6 degC respectively. DySn[sub 3] exists in a narrow temperature range, in two polymorphous modifications. The transformation [beta]-DySn[sub 3] [yields] [alpha]-DySn[sub 3] occurs at 608 [+-] 12 degC, and at 499 [+-]2 degC [alpha]-DySn[sub 3] decomposes to DySn[sub 2] and the tin-rich melt. The dysprosium-rich eutectic crystallizes at 1204 [+-]10 degC and contains 13 at.% tin. The solid-state solubility of tin in dysprosium is about 3 at.%, and that of dysprosium in tin is negligible.

  5. Towards a new measurement of parity violation in dysprosium

    CERN Document Server

    Leefer, N; Antypas, D; Budker, D

    2014-01-01

    The dysprosium parity violation experiment concluded nearly 17 years ago with an upper limit on weak interaction induced mixing of nearly degenerate, opposite parity states in atomic dysprosium. While that experiment was limited in sensitivity by statistics, a new apparatus constructed in the interim for radio-frequency spectroscopy is expected to provide significant improvements to the statistical sensitivity. Preliminary work from the new PV experiment in dysprosium is presented with a discussion of the current statistical sensitivity and outlook.

  6. Dysprosium Modification of Cobalt Ferrite Ionic Magnetic Fluids

    Institute of Scientific and Technical Information of China (English)

    JIANG Rong-li; LIU Yong-chao; GENG Quan-rong; ZHAO Wen-tao

    2005-01-01

    Dysprosium composite cobalt ferrite ionic magnetic fluids were prepared by precipitation in the presence of Tri-sodium citrate. Influence of dysprosium modification on magnetic property is studied. The result shows that magnetic response toward exterior magnetic field can be improved by adding Dy3+. Studies also show that the increase of reaction temperature may improve the modification effect of dysprosium. By adding dysprosium ions, the average diameter of the magnetic nanoparticles will be decreased evidently. It is clear that the particles appear as balls, Cobalt ferrite with sizes of 12-15 nm, rare earth composite cobalt ferrite with sizes of 6-8 nm.

  7. Can a dysprosium shortage threaten green energy technologies?

    NARCIS (Netherlands)

    Hoenderdaal, S.; Tercero Espinoza, L.; Marschneider-Weidemann, F.; Crijns - Graus, Wina

    2013-01-01

    Dysprosium, one of the various rare earth elements, is currently for more than 99% mined in China. As China is reducing its exports, new mining projects outside of China are needed to sustain supply and meet future demands. Dysprosium is mainly used in permanent magnets to retain the magnet's streng

  8. Phenalenyl-based mononuclear dysprosium complexes

    Directory of Open Access Journals (Sweden)

    Yanhua Lan

    2016-07-01

    Full Text Available The phenalenyl-based dysprosium complexes [Dy(PLN2(HPLNCl(EtOH] (1, [Dy(PLN3(HPLN]·[Dy(PLN3(EtOH]·2EtOH (2 and [Dy(PLN3(H2O2]·H2O (3, HPLN being 9-hydroxy-1H-phenalen-1-one, have been synthesized. All compounds were fully characterized by means of single crystal X-ray analysis, paramagnetic 1H NMR, MALDI-TOF mass spectrometry, UV–vis spectrophotometry and magnetic measurements. Both static (dc and dynamic (ac magnetic properties of these complexes have been investigated, showing slow relaxation of magnetization, indicative of single molecule magnet (SMM behavior. Attempts to synthesize sublimable phenalenyl-based dysprosium complexes have been made by implementing a synthetic strategy under anhydrous conditions. The sublimed species were characterized and their thermal stability was confirmed. This opens up the possibility to deposit phenalenyl-based lanthanides complexes by sublimation onto surfaces, an important prerequisite for ongoing studies in molecular spintronics.

  9. Anisotropy in the Interaction of Ultracold Dysprosium

    CERN Document Server

    Kotochigova, Svetlana

    2011-01-01

    The nature of the interaction between ultracold atoms with a large orbital and spin angular momentum has attracted considerable attention. It was suggested that such interactions can lead to the realization of exotic states of highly correlated matter. Here, we report on a theoretical study of the competing anisotropic dispersion, magnetic dipole-dipole, and electric quadrupole-quadrupole forces between two dysprosium atoms. Each dysprosium atom has an orbital angular momentum L=6 and magnetic moment $\\mu=10\\mu_B$. We show that the dispersion coefficients of the ground state adiabatic potentials lie between 1865 a.u. and 1890 a.u., creating a non-negligible anisotropy with a spread of 25 a.u. and that the electric quadrupole-quadrupole interaction is weak compared to the other interactions. We also find that for interatomic separations $R< 50\\,a_0$ both the anisotropic dispersion and magnetic dipole-dipole potential are larger than the atomic Zeeman splittings for external magnetic fields of order 10 G to ...

  10. Anisotropic magnetic properties of dysprosium iron garnet (DyIG)

    Energy Technology Data Exchange (ETDEWEB)

    Lahoubi, M; Younsi, W; Soltani, M-L [Department of Physics, Badji-Mokhtar University, BP 12 - 23000 Annaba (Algeria); Ouladdiaf, B, E-mail: mlahoubi@gmail.co [Institut Laue Langevin, BP 156 - 38042 Grenoble Cedex 9 (France)

    2010-01-01

    The magnetic properties of dysprosium iron garnet (DyIG) have been studied by performing high resolution powder neutron diffraction experiments and high dc fields magnetizations on single crystals. Among all the reflections (hkl) indexed in the nuclear cubic space group (CSG) Ia 3-bar d with h+k+l=2n and k=[000], the superstructure lines (hkl)* forbidden by the symmetry (222)* and (622)* are not observed in the patterns at all temperatures. The pattern at 130 K is well interpreted within the magnetic modes F belonging to the irreducible representation (IR) T{sub 1g} of the CSG and identified to the room temperature ferrimagnetic Neel model. The high magnetic field behavior of the spontaneous collinear magnetic structure (MS) along the easy axis (EA) <111> is isotropic. Below 130 K, the patterns exhibit additional magnetic superstructure lines. They are associated to the appearance of the spontaneous non collinear MS which is described in the subgroup of the CSG, R 3-bar c within the IR A{sub 2g}. A strong magnetization anisotropy (MA) is observed at 1.5 K in the low symmetry phases were the spin reorientation transition (SR) occur at T{sub RS}=14.5 K. The onset of MA is detected below two characteristic temperatures, Ta{sub 1}=125 K and Ta{sub 2}=75 K respectively to the hard axis (HA) <100> and <110>. Symmetry arguments are used in the framework of the theory of representation analysis (RA) applied to the subgroup of R 3-bar c, C2/c within the IR A{sub g}. It seems that this MA results essentially from the difference between the spontaneous non collinear MS and the field induced (FI) configurations. All results are discussed with previous neutrons studies.

  11. First search for double $\\beta$ decay of dysprosium

    CERN Document Server

    Belli, P; Cappella, F; Cerulli, R; Danevich, F A; d'Angelo, S; Di Vacri, M L; Incicchitti, A; Laubenstein, M; Nagorny, S S; Nisi, S; Tolmachev, A V; Tretyak, V I; Yavetskiy, R P

    2011-01-01

    A search for double $\\beta$ decay of dysprosium was realized for the first time with the help of an ultra low-background HP Ge $\\gamma$ detector. After 2512 h of data taking with a 322 g sample of dysprosium oxide limits on double beta processes in $^{156}$Dy and $^{158}$Dy have been established on the level of $T_{1/2}\\geq 10^{14}-10^{16}$ yr. Possible resonant double electron captures in $^{156}$Dy and $^{158}$Dy were restricted on a similar level. As a by-product of the experiment we have measured the radioactive contamination of the Dy$_2$O$_3$ sample and set limits on the $\\alpha$ decay of dysprosium isotopes to the excited levels of daughter nuclei as $T_{1/2}\\geq 10^{15} - 10^{17}$ yr.

  12. Neutron resonance parameters of dysprosium isotopes using neutron capture yields

    Energy Technology Data Exchange (ETDEWEB)

    Shin, S. G.; Kye, Y. U.; Cho, M. H. [POSTECH, Pohang (Korea, Republic of); Namkung, W. [Pohang Accelerator Laboratory, Pohang (Korea, Republic of); Kim, G. N. [Kyungpook National University, Daegu (Korea, Republic of); Lee, M. W.; Kang, Y. R. [Dongnam Inst. Of Radiological and Medical Science, Busan (Korea, Republic of)

    2015-10-15

    Dysprosium is used in the field of nuclear reactor system because it has a very large thermal neutron absorption cross-section. The dysprosium alloyed with special stainless steels is attractive for control in nuclear reactor because of the ability to absorb neutrons readily without swelling or contracting over time and its high melting point. Dysprosium is also one of fission products from the thermal fission of {sup 234}U, {sup 233}U, and {sup 239}Pu. The fission products are accumulated in the reactor core by the burn-up of the nuclear fuel and the poison effect is increased. Therefore, it is required to understand how Dysprosium as both a poison and an absorbing material in the control rod has an effect on the neutron population in a nuclear reactor system over all energy regions. Neutron Capture experiments on Dy isotopes were performed at the electron linear accelerator (LINAC) facility of the Rensselear Polytechnic Institute (RPI) in the neutron energy region from 10 eV to 1 keV. Resonance parameters were extracted by fitting the neutron capture data using the SAMMY multilevel R-matrix Bayesian code.

  13. Properties of Polydisperse Tin-doped Dysprosium and Indium Oxides

    Directory of Open Access Journals (Sweden)

    Malinovskaya Tatyana

    2017-01-01

    Full Text Available The results of investigations of the complex permittivity, diffuse-reflectance, and characteristics of crystal lattices of tin-doped indium and dysprosium oxides are presented. Using the methods of spectroscopy and X-ray diffraction analysis, it is shown that doping of indium oxide with tin results in a significant increase of the components of the indium oxide complex permittivity and an appearance of the plasma resonance in its diffuse-reflectance spectra. This indicates the appearance of charge carriers with the concentration of more than 1021 cm−3 in the materials. On the other hand, doping of the dysprosium oxide with the same amount of tin has no effect on its optical and electromagnetic properties.

  14. Dysprosium titanate as an absorber material for control rods

    Science.gov (United States)

    Risovany, V. D.; Varlashova, E. E.; Suslov, D. N.

    2000-09-01

    Disprosium titanate is an attractive control rod material for the thermal neutron reactors. Its main advantages are: insignificant swelling, no out-gassing under neutron irradiation, rather high neutron efficiency, a high melting point (˜1870°C), non-interaction with the cladding at temperatures above 1000°C, simple fabrication and easily reprocessed non-radioactive waste. It can be used in control rods as pellets and powder. The disprosium titanate control rods have worked off in the MIR reactor for 17 years, in VVER-1000 - for 4 years without any operating problems. After post-irradiation examinations this type of control rod having high lifetime was recommended for the VVER and RBMK. The paper presents the examination results of absorber element dummies containing dysprosium titanate, irradiated in the SM reactor to the neutron fluence of 3.4×10 22 cm -2 ( E>0.1 MeV) and, also, the data on structure, thermal-physical properties of dysprosium titanate, efficiency of dysprosium titanate control rods.

  15. A comparative study of donor formation in dysprosium, holmium, and erbium implanted silicon

    Energy Technology Data Exchange (ETDEWEB)

    Emtsev, V.V.; Emtsev, V.V. Jr.; Poloskin, D.S.; Shek, E.I.; Sobolev, N.A. [Division of Solid State Electronics, Ioffe Physicotechnical Institute, Russian Academy of Sciences, 194021 St. Petersburg (Russian Federation)

    1998-12-01

    Formation of donor centers in Czochralski grown silicon doped with dysprosium, holmium, and erbium is discussed. Donor states of three kinds are introduced in the implanted layers after annealing at T=700C. Shallow donor states with ionization energies between 20 and 40 meV are attributed to oxygen -related thermal donors. Other donor centers in the energy range of E{sub C}-(60...70) meV and E{sub C}-(100...120) meV appear to be dependent on dopants. After a 900C anneal strong changes in the donor formation are observed only in silicon doped with erbium. Instead of donors at E{sub C}-(118{+-}5) meV, new donor centres at E{sub C}-(145{+-}5) meV are formed. Reportedly, the latter ones are involved in the excitation process of the Er{sup 3+} ions with a characteristic luminescence line at {approx}1.54 {mu}m. (Copyright (c) 1998 Elsevier Science B.V., Amsterdam. All rights reserved.)

  16. 145 - 147 Chonoko

    African Journals Online (AJOL)

    DR. AMIN

    2011-06-01

    Jun 1, 2011 ... Bayero Journal of Pure and Applied Sciences, 4(1): 145 - 147. Received: ... Department of Applied Science College of Science and Technology Kaduna Polytechnic, Kaduna. ... pumpkins has been widely used in culinary and.

  17. Dysprosium electrodeposition from a hexaalkylguanidinium-based ionic liquid

    Science.gov (United States)

    Berger, Claudia A.; Arkhipova, Maria; Maas, Gerhard; Jacob, Timo

    2016-07-01

    The rare-earth element dysprosium (Dy) is an important additive that increases the magnetocrystalline anisotropy of neodymium magnets and additionally prevents from demagnetizing at high temperatures. Therefore, it is one of the most important elements for high-tech industries and is mainly used in permanent magnetic applications, for example in electric vehicles, industrial motors and direct-drive wind turbines. In an effort to develop a more efficient electrochemical technique for depositing Dy on Nd-magnets in contrast to commonly used costly physical vapor deposition, we investigated the electrochemical behavior of dysprosium(iii) trifluoromethanesulfonate in a custom-made guanidinium-based room-temperature ionic liquid (RTIL). We first examined the electrodeposition of Dy on an Au(111) model electrode. The investigation was carried out by means of cyclic voltammetry (CV) and X-ray photoelectron spectroscopy (XPS). The initial stages of metal deposition were followed by in situ scanning tunneling microscopy (STM). CV measurements revealed a large cathodic reduction peak, which corresponds to the growth of monoatomic high islands, based on STM images taken during the initial stages of deposition. XPS identified these deposited islands as dysprosium. A similar reduction peak was also observed on an Nd-Fe-B substrate, and positively identified as deposited Dy using XPS. Finally, we varied the concentration of the Dy precursor, electrolyte flow and temperature during Dy deposition and demonstrated that each of these parameters could be used to increase the thickness of the Dy deposit, suggesting that these parameters could be tuned simultaneously in a temperature-controlled flow cell to enhance the thickness of the Dy layer.The rare-earth element dysprosium (Dy) is an important additive that increases the magnetocrystalline anisotropy of neodymium magnets and additionally prevents from demagnetizing at high temperatures. Therefore, it is one of the most important

  18. Dysprosium detector for neutron dosimetry in external beam radiotherapy

    Science.gov (United States)

    Ostinelli, A.; Berlusconi, C.; Conti, V.; Duchini, M.; Gelosa, S.; Guallini, F.; Vallazza, E.; Prest, M.

    2014-09-01

    Radiotherapy treatments with high-energy (>8 MeV) photon beams are a standard procedure in clinical practice, given the skin and near-target volumes sparing effect, the accurate penetration and the uniform spatial dose distribution. On the other hand, despite these advantages, neutrons may be produced via the photo-nuclear (γ,n) reactions of the high-energy photons with the high-Z materials in the accelerator head, in the treatment room and in the patient, resulting in an unwanted dose contribution which is of concern, given its potential to induce secondary cancers, and which has to be monitored. This work presents the design and the test of a portable Dysprosium dosimeter to be used during clinical treatments to estimate the "in vivo" dose to the patient. The dosimeter has been characterized and validated with tissue-equivalent phantom studies with a Varian Clinical iX 18 MV photon beam, before using it with a group of patients treated at the S. Anna Hospital in Como. The working principle of the dosimeter together with the readout chain and the results in terms of delivered dose are presented.

  19. Dysprosium detector for neutron dosimetry in external beam radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Ostinelli, A.; Berlusconi, C.; Conti, V.; Duchini, M.; Gelosa, S. [Medical Physics - Sant' Anna Hospital, Como (Italy); Guallini, F. [EL.SE s.r.l. (Italy); Vallazza, E. [INFN, Trieste (Italy); Prest, M. [University of Insubria, Como (Italy)

    2014-09-21

    Radiotherapy treatments with high-energy (>8MeV) photon beams are a standard procedure in clinical practice, given the skin and near-target volumes sparing effect, the accurate penetration and the uniform spatial dose distribution. On the other hand, despite these advantages, neutrons may be produced via the photo-nuclear (γ,n) reactions of the high-energy photons with the high-Z materials in the accelerator head, in the treatment room and in the patient, resulting in an unwanted dose contribution which is of concern, given its potential to induce secondary cancers, and which has to be monitored. This work presents the design and the test of a portable Dysprosium dosimeter to be used during clinical treatments to estimate the “in vivo” dose to the patient. The dosimeter has been characterized and validated with tissue-equivalent phantom studies with a Varian Clinical iX 18 MV photon beam, before using it with a group of patients treated at the S. Anna Hospital in Como. The working principle of the dosimeter together with the readout chain and the results in terms of delivered dose are presented.

  20. Low Field Magnetic and Thermal Hysteresis in Antiferromagnetic Dysprosium

    Directory of Open Access Journals (Sweden)

    Iuliia Liubimova

    2017-06-01

    Full Text Available Magnetic and thermal hysteresis (difference in magnetic properties on cooling and heating have been studied in polycrystalline Dy (dysprosium between 80 and 250 K using measurements of the reversible Villari effect and alternating current (AC susceptibility. We argue that measurement of the reversible Villari effect in the antiferromagnetic phase is a more sensitive method to detect magnetic hysteresis than the registration of conventional B(H loops. We found that the Villari point, recently reported in the antiferromagnetic phase of Dy at 166 K, controls the essential features of magnetic hysteresis and AC susceptibility on heating from the ferromagnetic state: (i thermal hysteresis in AC susceptibility and in the reversible Villari effect disappears abruptly at the temperature of the Villari point; (ii the imaginary part of AC susceptibility is strongly frequency dependent, but only up to the temperature of the Villari point; (iii the imaginary part of the susceptibility drops sharply also at the Villari point. We attribute these effects observed at the Villari point to the disappearance of the residual ferromagnetic phase. The strong influence of the Villari point on several magnetic properties allows this temperature to be ranked almost as important as the Curie and Néel temperatures in Dy and likely also for other rare earth elements and their alloys.

  1. A Low-Symmetry Dysprosium Metallocene Single-Molecule Magnet with a High Anisotropy Barrier.

    Science.gov (United States)

    Pugh, Thomas; Chilton, Nicholas F; Layfield, Richard A

    2016-09-05

    The single-molecule magnet (SMM) properties of the isocarbonyl-ligated dysprosium metallocene [Cp*2 Dy{μ-(OC)2 FeCp}]2 (1Dy ), which contains a rhombus-shaped Dy2 Fe2 core, are described. Combining a strong axial [Cp*](-) ligand field with a weak equatorial field consisting of the isocarbonyl ligands leads to an anisotropy barrier of 662 cm(-1) in zero applied field. The dominant thermal relaxation pathways in 1Dy involves at least the fourth-excited Kramers doublet, thus demonstrating that prominent SMM behavior can be observed for dysprosium in low-symmetry environments.

  2. Malonate complexes of dysprosium: synthesis, characterization and application for LI-MOCVD of dysprosium containing thin films.

    Science.gov (United States)

    Milanov, Andrian P; Seidel, Rüdiger W; Barreca, Davide; Gasparotto, Alberto; Winter, Manuela; Feydt, Jürgen; Irsen, Stephan; Becker, Hans-Werner; Devi, Anjana

    2011-01-07

    A series of malonate complexes of dysprosium were synthesized as potential metalorganic precursors for Dy containing oxide thin films using chemical vapor deposition (CVD) related techniques. The steric bulkiness of the dialkylmalonato ligand employed was systematically varied and its influence on the resulting structural and physico-chemical properties that is relevant for MOCVD was studied. Single crystal X-ray diffraction analysis revealed that the five homoleptic tris-malonato Dy complexes (1-5) are dimers with distorted square-face bicapped trigonal-prismatic geometry and a coordination number of eight. In an attempt to decrease the nuclearity and increase the solubility of the complexes in various solvents, the focus was to react these dimeric complexes with Lewis bases such as 2,2'-biypridyl and pyridine (6-9). This resulted in monomeric tris-malonato mono Lewis base adduct complexes with improved thermal properties. Finally considering the ease of synthesis, the monomeric nature and promising thermal characteristics, the silymalonate adduct complex [Dy(dsml)(3)bipy] (8) was selected as single source precursor for growing DySi(x)O(y) thin films by liquid injection metalorganic chemical vapor deposition (LI-MOCVD) process. The as-deposited films were analyzed for their morphology and composition by scanning electron microscopy (SEM), energy dispersive X-ray (EDX) analysis, Rutherford backscattering (RBS) analysis and X-ray photoelectron spectroscopy.

  3. Exploration of dysprosium: the most critical element for Japan

    Science.gov (United States)

    Watanabe, Y.

    2012-04-01

    Dysprosium (Dy), one of the heavy rare earth elements, is used mainly as an additive for NdFeB permanent magnets which are installed in various modern industrial products such as voice coil motors in computers, factory automation machinery, hybrid and electric vehicles, home electronics, and wind turbine, to improve heat resistance of the magnets. Dy has been produced about 2,000t per year from the ores from ion adsorption type deposits in southern China. However, the produced amount of Dy was significantly reduced in 2011 in China due to reservation of heavy rare earth resources and protection of natural environment, resulting in soaring of Dy price in the world. In order to respond the increasing demand of Dy, unconventional supply sources are inevitably developed, in addition to heavy rare earth enriched ion adsorption type deposits outside China. Heavy rare earth elements including Dy are dominantly hosted in xenotime, fergusonite, zircon, eudialyte, keiviite, kainosite, iimoriite, etc. Concentration of xenotime is found in placer deposits in Malaysia and India, hydrothermal deposits associated with unconformity-type uranium mineralization (Athabasca basin in Canada, Western Australia), iron-oxide fluorite mineralization (South Africa) and Sn-bearing alkaline granite (Brazil). Zircon and fergusontie concentration is found as igneous and hydrothermal products in peralkaline syenite, alkaline granite and pegmatite (e.g., Nechalacho in Canada). Eudialyte concentration is found in some peralkaline syenite bodies in Greenland, Canada, Sweden and Russia. Among these sources, large Dy resources are estimated in the deposits hosted in peralkaline rocks (Nechalacho: 79,000t, Kvanefjeld: 49,000t, Norra Karr: 15,700t, etc.) compared to the present demand of Dy. Thus, Dy will be supplied from the deposits associated with peralkaline and alkaline deposits in future instead of ion adsorption type deposits in southern China.

  4. Long afterglow of trivalent dysprosium doped strontium aluminate

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Yuan Ming, E-mail: dongshanisland@126.com [School of Mathematics and Physics, Changzhou University, Jiangsu 213164 (China); Ma, Qing-lan [School of Mathematics and Physics, Changzhou University, Jiangsu 213164 (China); School of Electronics and Information, Nantong University, Jiangsu 226019 (China)

    2015-04-15

    Trivalent dysprosium doped strontium aluminate (SrA1{sub 2}O{sub 4}:Dy{sup 3+}) was synthesized via the sol–gel combustion method to realize green afterglow in the absence of Eu{sup 2+} luminescent centers. The morphology, crystal structure, photoluminescence and long afterglow of the SrAl{sub 2}O{sub 4}:Dy{sup 3+} were characterized with scanning electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy and photoluminescence spectroscopy, respectively. The bluish-green photoluminescence of SrAl{sub 2}O{sub 4}:Dy{sup 3+} consists of a broad emission band centered at about 520 nm and two characteristic emissions of Dy{sup 3+} ions centered at 480 and 575 nm, respectively. The green afterglow of SrAl{sub 2}O{sub 4}:Dy{sup 3+} is a broad emission band centered at around 520 nm, and the lifetime extracted from afterglow decay is found to be 53 s. The mechanism on the green afterglow from SrAl{sub 2}O{sub 4}:Dy{sup 3+} is discussed in terms of the possible defect levels in the host. - Highlights: • Broad band long-lasting afterglow is observed in SrAl{sub 2}O{sub 4}:Dy{sup 3+} phosphors. • Characteristic emissions of Dy{sup 3+} ions are superimposed on the broad PL of phosphors. • Dy{sup 3+} ions can also act as luminescent centers in addition to electron traps. • A mechanism on long afterglow of SrAl{sub 2}O{sub 4}:Dy{sup 3+} is proposed without Eu{sup 2+} activator.

  5. Low temperature spin reorientation in dysprosium iron garnet

    Energy Technology Data Exchange (ETDEWEB)

    Lahoubi, M; Younsi, W; Soltani, M L [Department of Physics, Badji-Mokhtar University, BP-12 Annaba, 23000 (Algeria); Voiron, J; Schmitt, D, E-mail: mlahoubi@gmail.co [Louis Neel Laboratory, CNRS-UJF, BP-166, 38042 Grenoble Cedex 9 (France)

    2009-03-01

    The spin reorientation (SR) phase transition in dysprosium iron garnet (Dy{sub 3}Fe{sub 5}O{sub 12} or DyIG) have been studied by specific heat C{sub p}(T) and high field magnetisation measurements M{sub T}(H) and M{sub H}(T) on single crystals at low temperature. A first order SR is observed with a sharp jump at T{sub SR} = 14.5+-0.5 K in the C{sub p}(T) curve which corresponds to a spontaneous change from the high temperature (HT) easy direction (111) to an (uuw) angular low temperature (LT) phases. Above T{sub SR}, the magnetic structure is described by the irreducible representation (IR) A{sub 2g} of the rhombohedral space group R 3 c. Below T{sub SR}, the magnetic structure changes in the monoclinic the space group C2/c with the IR A{sub g}. When the field H is kept aligned along the hard symmetry directions (100) and (110), we obtain respectively the variation of the angular positions theta(T) and theta'(T) from the total spontaneous magnetisation down to 1.5 K (theta = 39.23 deg. and theta' = 30.14 deg.) and the results are in good agreement with the previous observations in low fields. When the sample is allowed to rotate freely on itself, the critical field H{sub c}(T) between the HT(111) and the LT(uuw) angular phases permits us to precise the transition line up to 15 T and 40 K between the so called canted field induced (FI) and the associated collinear magnetic phases. The experimental magnetic phase diagram (MPD) is precisely determined in the (H{sub c}-T) plane and the domains of the existence and the stability of the two magnetic phases are specified.

  6. 46 CFR 28.145 - Distress signals.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Distress signals. 28.145 Section 28.145 Shipping COAST... VESSELS Requirements for All Vessels § 28.145 Distress signals. Except as provided by 28.305, each vessel must be equipped with the distress signals specified in table 28.145. Table 28.145—Distress Signals...

  7. Dysprosium-containing layered double hydroxides nanoparticles intercalated with biologically active species as an approach for theranostic systems

    KAUST Repository

    Arratia-Quijada, Jenny

    2015-10-23

    A layered double hydroxide structure including dysprosium cations was prepared by co-precipitation. The nanoparticles showed a linear relationship with the reciprocal relaxation spin-lattice (T1) time of water protons which is reflected as contrast in aqueous suspensions analyzed by magnetic resonance imaging. The interlayer space of dysprosium containing LDH was successfully intercalated with folate, ibuprofen and gallate ions, which are key molecules for recognition of some cancer cells and treatment of diseases. The paramagnetic property of the dysprosium-containing LDH detected in this work beside the ability to transport drugs open up the opportunity to design theranostic materials in a single crystal phase with nanometric dimensions.

  8. Effects of Dysprosium Oxide Doping on Microstructure and Properties of Barium Titanate Ceramic

    Institute of Scientific and Technical Information of China (English)

    Pu Yongping; Ren Huijun; Chen Wei; Chen Shoutian

    2005-01-01

    Different amounts of dysprosium oxide were incorporated into barium titanate powders synthesized by hydrothermal method. Relations of substitution behaviors and lattice parameters with solid-solubility were studied. Furthermore, the influences of dysprosium oxide doping fraction on grain size and dielectric properties of barium titanate ceramic, including dielectric constant and breakdown electric field strength, were investigated via scanning electron microscope, X-ray diffraction and electric property tester. The results show that dysprosium oxide can restrain abnormal grain growth during sintering and that fine-grained and high density of barium titanate ceramic can result in excellent dielectric properties. As mass fraction of dysprosium oxide is 0.6%, the lattice parameters of grain increase to the maximum because of the lowest vacancy concentration. The electric property parameters are cited as following: dielectric constant (25 ℃) reaches 4100, the change in relative dielectric constant with temperature is -10% to 10% within the range of -15~100 ℃, breakdown electric field strength (alternating current) achieves 3.2 kV·mm-1, which can be used in manufacturing high voltage ceramic capacitors.

  9. Sandwich-type tetrakis(phthalocyaninato) dysprosium-cadmium quadruple-decker SMM.

    Science.gov (United States)

    Wang, Hailong; Qian, Kang; Wang, Kang; Bian, Yongzhong; Jiang, Jianzhuang; Gao, Song

    2011-09-14

    Homoleptic tetrakis[2,3,9,10,16,17,23,24-octa(butyloxy)phthalocyaninato] dysprosium-cadmium quadruple-decker complex 1 was isolated in relatively good yield of 43% from a simple one-pot reaction. This compound represents the first sandwich-type tetrakis(phthalocyaninato) rare earth-cadmium quadruple-decker SMM that has been structurally characterized.

  10. In situ characterization of the nitridation of dysprosium during mechanochemical processing

    Energy Technology Data Exchange (ETDEWEB)

    Jaques, Brian J.; Osterberg, Daniel D.; Alanko, Gordon A.; Tamrakar, Sumit; Smith, Cole R.; Hurley, Michael F.; Butt, Darryl P., E-mail: DarrylButt@BoiseState.edu

    2015-01-15

    Highlights: • A nitridation reaction in a high energy planetary ball mill was monitored in situ. • Dysprosium mononitride was synthesized from Dy at low temperatures in short times. • Ideal gas law and in situ temperature and pressure used to assess reaction extent. • It is proposed that reaction rate is proportional to the creation of new surface. - Abstract: Processing of advanced nitride ceramics traditionally requires long durations at high temperatures and, in some cases, in hazardous atmospheres. In this study, dysprosium mononitride (DyN) was rapidly formed from elemental dysprosium in a closed system at ambient temperatures. An experimental procedure was developed to quantify the progress of the nitridation reaction during mechanochemical processing in a high energy planetary ball mill (HEBM) as a function of milling time and intensity using in situ temperature and pressure measurements, SEM, XRD, and particle size analysis. No intermediate phases were formed. It was found that the creation of fresh dysprosium surfaces dictates the rate of the nitridation reaction, which is a function of milling intensity and the number of milling media. These results show clearly that high purity nitrides can be synthesized with short processing times at low temperatures in a closed system requiring a relatively small processing footprint.

  11. 7 CFR 65.145 - Ginseng.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Ginseng. 65.145 Section 65.145 Agriculture Regulations..., AND GINSENG General Provisions Definitions § 65.145 Ginseng. Ginseng means ginseng root of the genus Panax. ...

  12. Dysprosium complexes with the tetraphenylporphyrin macrocyclic ligand; Complejos de disprosio con el ligante macrociclico tetrafenilporfirina

    Energy Technology Data Exchange (ETDEWEB)

    Martinez M, V.; Padilla, J.; Ramirez, F.M

    1992-04-15

    In this report, the results obtained on the synthesis, characterization and study of the chemical behavior of dysprosium complex with the acetylacetone chelating agent (Hacac) and the tetraphenylporphyrin macrocyclic ligand (H{sub 2}TFP) are given. Based on the literature but according to our necessities and interest, the appropriate methodology settled down from the synthesis of prime matters until the obtaining and characterization of the products. The acetyl acetonate complex was obtained of mono hydrated dysprosium [Dy(acac){sub 3}. H{sub 2}0] and trihydrated [Dy(acac){sub 3} .3 H{sub 2}0], the mono tetra phenyl porphyrinate [Dy(TFP)(acac). 2 ac] the double sandwich of the dysprosium porphyrinate [Dy(TFP){sub 2}] and the triple sandwich of the dysprosium porphyrinate [Dy(TFP){sub 3}. 2 TCB] (TCB = trichlorobenzene). Its were characterized by their melting points, solubility, IR, UV, TGA and DTA both first and besides the techniques already mentioned for NMR'H, RPE and Magnetic susceptibility the three last complexes. From the spectroscopic point of view, IR and RPE its suggested the existence of a complex of inverse mixed valence [Dy(TFP){sup 2-} (TFP) {sup 1-}] for the Dy(TFP){sub 2} as a result of the existence of the free radical (TFP' {sup 1-} and that it was not in none of the other porphyrin compounds. In the NMR'H spectra of the compounds were not observed signals in the region from 0 to 10 ppm that which shows that the dysprosium complexes in special those of the porphyrin type are highly paramagnetic and its could be used as displacement reagents, creators of images and contrast agents of great utility in these days in studies of NMR, technique today by today used in medical diagnoses. (Author)

  13. Synthesis, structural characterization and in vitro testing of dysprosium containing silica particles as potential MRI contrast enhancing agents

    Science.gov (United States)

    Chiriac, L. B.; Trandafir, D. L.; Turcu, R. V. F.; Todea, M.; Simon, S.

    2016-11-01

    The work is focused on synthesis and structural characterization of novel dysprosium-doped silica particles which could be considered as MRI contrast agents. Sol-gel derived silica rich particles obtained via freeze-drying and spray-drying processing methods were structurally characterized by XRD, 29Si MAS-NMR and XPS methods. The occurrence of dysprosium on the outermost layer of dysprosium containing silica particles was investigated by XPS analysis. The MRI contrast agent characteristics have been tested using RARE-T1 and RARE-T2 protocols. The contrast of MRI images delivered by the investigated samples was correlated with their local structure. Dysprosium disposal on microparticles with surface structure characterised by decreased connectivity of the silicate network units favours dark T2-weighted MRI contrast properties.

  14. Dysprosium-containing layered double hydroxides nanoparticles intercalated with biologically active species as an approach for theranostic systems

    Energy Technology Data Exchange (ETDEWEB)

    Arratia-Quijada, Jenny [Departamento de Ciencias de la Salud, Centro Universitario Tonalá, Universidad de Guadalajara, Av. Nuevo Periférico No. 555, C.P. 48525, Tonalá, Jalisco (Mexico); Sánchez Jiménez, Cecilia [Departamento de Química, Universidad de Guadalajara, Boulevard Marcelino García Barragán 1421, C.P. 44430, Guadalajara, Jalisco (Mexico); Gurinov, Andrey [Research Resources Center for Magnetic Resonance, St. Petersburg State University, Universitetskiy pr. 26, 198504 St. Petersburg (Russian Federation); NMR Core Lab, King Abdullah University of Science and Technology, Thuwal 23955-6900 (Saudi Arabia); Pérez Centeno, Armando; Ceja Andrade, Israel [Departamento de Física, Universidad de Guadalajara, Boulevard Marcelino García Barragán 1421, C.P. 44430, Guadalajara, Jalisco (Mexico); Carbajal Arízaga, Gregorio Guadalupe, E-mail: gregoriocarbajal@yahoo.com.mx [Departamento de Química, Universidad de Guadalajara, Boulevard Marcelino García Barragán 1421, C.P. 44430, Guadalajara, Jalisco (Mexico)

    2016-01-15

    Graphical abstract: - Highlights: • LDH structure including dysprosium was prepared by co-precipitation. • LDH was capable to produce contrast in the T1 mode of MRI. • LDH were intercalated with folate, ibuprofen and gallate ions. - Abstract: A layered double hydroxide structure including dysprosium cations was prepared by co-precipitation. The nanoparticles showed a linear relationship with the reciprocal relaxation spin-lattice (T1) time of water protons which is reflected as contrast in aqueous suspensions analyzed by magnetic resonance imaging. The interlayer space of dysprosium containing LDH was successfully intercalated with folate, ibuprofen and gallate ions, which are key molecules for recognition of some cancer cells and treatment of diseases. The paramagnetic property of the dysprosium-containing LDH detected in this work beside the ability to transport drugs open up the opportunity to design theranostic materials in a single crystal phase with nanometric dimensions.

  15. Slow magnetic relaxation in a hydrogen-bonded 2D array of mononuclear dysprosium(III) oxamates.

    Science.gov (United States)

    Fortea-Pérez, Francisco R; Vallejo, Julia; Julve, Miguel; Lloret, Francesc; De Munno, Giovanni; Armentano, Donatella; Pardo, Emilio

    2013-05-01

    The reaction of N-(2,6-dimethylphenyl)oxamic acid with dysprosium(III) ions in a controlled basic media afforded the first example of a mononuclear lanthanide oxamate complex exhibiting a field-induced slow magnetic relaxation behavior typical of single-ion magnets (SIMs). The hydrogen-bond-mediated self-assembly of this new bifunctional dysprosium(III) SIM in the solid state provides a unique example of 2D hydrogen-bonded polymer with a herringbone net topology.

  16. Dysprosium-Catalyzed Growth of Single-Walled Carbon Nanotube Arrays on Substrates

    Directory of Open Access Journals (Sweden)

    Qian Yong

    2009-01-01

    Full Text Available Abstract In this letter, we report that dysprosium is an effective catalyst for single-walled carbon nanotubes (SWNTs growth via a chemical vapor deposition (CVD process for the first time. Horizontally superlong well-oriented SWNT arrays on SiO2/Si wafer can be fabricated by EtOH-CVD under suitable conditions. The structure and properties are characterized by scanning electron microscopy, transition electron microscopy, Raman spectroscopy and atomic force microscopy. The results show that the SWNTs from dysprosium have better structural uniformity and better conductivity with fewer defects. This rare earth metal provides not only an alternative catalyst for SWNTs growth, but also a possible method to generate high percentage of superlong semiconducting SWNT arrays for various applications of nanoelectronic device.

  17. Direct Search for keV Sterile Neutrino Dark Matter with a Stable Dysprosium Target

    CERN Document Server

    Lasserre, T; Cribier, M; Merle, A; Mertens, S; Vivier, M

    2016-01-01

    We investigate a new method to search for keV-scale sterile neutrinos that could account for Dark Matter. Neutrinos trapped in our galaxy could be captured on stable $^{163}$Dy if their mass is greater than 2.83~keV. Two experimental realizations are studied, an integral counting of $^{163}$Ho atoms in dysprosium-rich ores and a real-time measurement of the emerging electron spectrum in a dysprosium-based detector. The capture rates are compared to the solar neutrino and radioactive backgrounds. An integral counting experiment using several kilograms of $^{163}$Dy could reach a sensitivity for the sterile-to-active mixing angle $\\sin^2\\theta_{e4}$ of $10^{-5}$ significantly exceeding current laboratory limits. Mixing angles as low as $\\sin^2\\theta_{e4} \\sim 10^{-7}$ / $\\rm m_{^{163}\\rm Dy}\\rm{(ton)}$ could possibly be explored with a real-time experiment.

  18. Preparation of Dysprosium Ferrite/Polyacrylamide Magnetic Composite Microsphere and Its Characterization

    Institute of Scientific and Technical Information of China (English)

    Hidehiro Kumazawa; Wang Zhifeng; Zhou Lanxiang; Zhang Hong; Li Yourong; Zhang Ming

    2005-01-01

    Using the technique of microemulsion polymerization with nano-reactor, dysprosium ferrite/polyacrylamide magnetic composite microsphere was prepared by one-step method in a single inverse microemulsion. The structure, average particle size, morphology of composite microsphere were characterized by FTIR, XRD, TEM and TGA. The magnetic responsibility of composite microsphere was also investigated. The results indicate that the magnetic composite microsphere possess high magnetic responsibility and suspension stability.

  19. {Delta}I = 2 energy staggering in normal deformed dysprosium nuclei

    Energy Technology Data Exchange (ETDEWEB)

    Riley, M.A.; Brown, T.B.; Archer, D.E. [Florida State Univ., Tallahassee, FL (United States)] [and others

    1996-12-31

    Very high spin states (I{ge}50{Dirac_h}) have been observed in {sup 155,156,157}Dy. The long regular band sequences, free from sharp backbending effects, observed in these dysprosium nuclei offer the possibility of investigating the occurence of any {Delta}I = 2 staggering in normal deformed nuclei. Employing the same analysis techniques as used in superdeformed nuclei, certain bands do indeed demonstrate an apparent staggering and this is discussed.

  20. Poly[[[μ3-N′-(carboxymethylethylenediamine-N,N,N′-triacetato]dysprosium(III] trihydrate

    Directory of Open Access Journals (Sweden)

    Xiaomei Zhuang

    2010-11-01

    Full Text Available In the title coordination polymer, {[Dy(C10H13N2O8]·3H2O}n, the dysprosium(III ion is coordinated by two N atoms and six O atoms from three different (carboxymethylethylenediaminetriacetate ligands in a distorted square-antiprismatic geometry. The ligands connect the metal atoms, forming layers parallel to the ab plane. O—H...O hydrogen bonds further assemble adjacent layers into a three-dimensional supramolecular network.

  1. Making two dysprosium atoms rotate - Einstein-de Haas effect revisited

    OpenAIRE

    Górecki, Wojciech; Rzążewski, Kazimierz

    2016-01-01

    We present a numerical study of the behaviour of two magnetic dipolar atoms trapped in a harmonic potential and exhibiting the standard Einstein-de Haas effect while subject to a time dependent homogeneous magnetic field. Using a simplified description of the short range interaction and the full expression for the dipole-dipole forces we show, that under experimentally realisable conditions two dysprosium atoms may be pumped to a high ($l>20$) value of the relative orbital angular momentum.

  2. Phosphor Dysprosium-Doped Layered Double Hydroxides Exchanged with Different Organic Functional Groups

    Directory of Open Access Journals (Sweden)

    David Ricardo Martínez Vargas

    2013-01-01

    Full Text Available The layers of a Zn/Al layered double hydroxide (LDH were doped with Dy3+ cations. Among some compositions, the Zn2+ : Al3+ : Dy3+ molar ratio equal to 30 : 9 : 1 presented a single crystalline phase. Organic anions with carboxylic, amino, sulfate, or phosphate functional groups were intercalated as single layers between LDH layers as confirmed by X-ray diffraction and infrared spectroscopy. Photoluminescence spectra of the nitrate intercalated LDH showed a wide emission band with strong intensity in the yellow region (around 574 nm, originated due to symmetry distortion of the octahedral coordination in dysprosium centers. Moreover, a broad red band emission was also detected apparently due to the presence of zinc oxide. The distorted symmetry of the dysprosium coordination environment, also confirmed by X-ray photoelectron spectroscopy analysis, was modified after the intercalation with phenyl phosphonate (PP, aspartate (Asp, adipate (Adip, and serinate (Ser anions; the emission as measured from PL spectra of these LDH was more intense in the blue region (ca. 486 nm, thus indicating an increase in symmetry of dysprosium octahedrons. The red emission band from zinc oxide kept the same intensity after intercalation of dodecyl sulfate (DDS. An additional emission of unknown origin at λ = 767 nm was present in all LDHs.

  3. Systematic study on surface and magnetostructural changes in Mn-substituted dysprosium ferrite by hydrothermal method

    Energy Technology Data Exchange (ETDEWEB)

    Rekha, G. [Department of Physics, College of Engineering Guindy, Anna University, Sardar Patel Road, Chennai 600025 (India); Tholkappiyan, R. [Department of Physics, College of Engineering Guindy, Anna University, Sardar Patel Road, Chennai 600025 (India); Department of Physics, College of Science, UAE University, Al-Ain 15551 (United Arab Emirates); Vishista, K., E-mail: raovishista@gmail.com [Department of Physics, College of Engineering Guindy, Anna University, Sardar Patel Road, Chennai 600025 (India); Hamed, Fathalla [Department of Physics, College of Science, UAE University, Al-Ain 15551 (United Arab Emirates)

    2016-11-01

    Highlights: • Garnet type Dy{sub 3}Fe{sub 5-x}Mn{sub x}O{sub 12} (x = 0–0.06) nanoparticles of 88.4–86.8 nm were synthesized by hydrothermal method. • The Dy, Mn, Fe and O elements in the ferrites were confirmed from XPS. • The multiple oxidation states of Fe and Mn ions, bonding energy and cationic distributions of the samples were examined by XPS. • The magnetic property shows ferromagnetic behavior from VSM technique. • The results from these studies are correlated with respect to Mn dopant. - Abstract: Dysprosium iron garnets are of scientific importance because of the wide range of magnetic properties that can be obtained in substituting dysprosium by a rare earth metal. In the present work, the effect of Mn substitution on magnetostructural changes in dysprosium ferrite nanoparticles is studied. Highly crystalline pure and Mn doped dysprosium ferrite nanoparticles were synthesized by hydrothermal method. The samples were calcined at 1100 °C for 2 h in air atmosphere which is followed by characterization using XRD, FT-IR analysis, SEM, XPS and VSM. The average crystallite size of synthesized samples were calculated by X-ray diffraction falls in the range of 88.4–86.8 nm and was found to be in cubic garnet structure. For further investigation of the structure and corresponding changes in the tetrahedral and octahedral stretching vibrational bonds, FT-IR was used. The synthesized samples consist of multiple oxidation (Fe{sup 3+} and Fe{sup 2+}) states for Fe ions and (Mn{sup 3+} and Mn{sup 2+}) Mn ions analyzed in three ways of Fe 2p and Mn 2p spectra from the XPS analysis. With respect to Mn dopant in Dy{sub 3}Fe{sub 5}O{sub 12}, the cationic distributions of elements were discussed from high resolution XPS spectra by peak position and shift, area, width. To find out the porous/void surface morphology of the sample, scanning electron microscopy was used. From XPS analysis, the presence of elements (Dy, Mn, Fe and O) and their composition in the

  4. Synthesis, structural characterization and in vitro testing of dysprosium containing silica particles as potential MRI contrast enhancing agents

    Energy Technology Data Exchange (ETDEWEB)

    Chiriac, L.B.; Trandafir, D.L. [Faculty of Physics & National Magnetic Resonance Center, Babeş-Bolyai University, Cluj-Napoca, RO-400084 (Romania); Interdisciplinary Research Institute on Bio-Nano-Sciences & Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca, RO-400084 (Romania); Turcu, R.V.F. [Faculty of Physics & National Magnetic Resonance Center, Babeş-Bolyai University, Cluj-Napoca, RO-400084 (Romania); Todea, M. [Interdisciplinary Research Institute on Bio-Nano-Sciences & Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca, RO-400084 (Romania); Simon, S., E-mail: simons@phys.ubbcluj.ro [Faculty of Physics & National Magnetic Resonance Center, Babeş-Bolyai University, Cluj-Napoca, RO-400084 (Romania); Interdisciplinary Research Institute on Bio-Nano-Sciences & Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca, RO-400084 (Romania)

    2016-11-01

    Highlights: • Dysprosium containing silica microparticles obtained by freeze and spray drying. • Higher structural units interconnection achieved in freeze vs. spray dried samples. • Dy occurance on the outermost layer of the microparticles evidenced by XPS. • Enhanced MRI contrast observed for freeze dried samples with 5% mol Dy{sub 2}O{sub 3}. - Abstract: The work is focused on synthesis and structural characterization of novel dysprosium-doped silica particles which could be considered as MRI contrast agents. Sol-gel derived silica rich particles obtained via freeze-drying and spray-drying processing methods were structurally characterized by XRD, {sup 29}Si MAS-NMR and XPS methods. The occurrence of dysprosium on the outermost layer of dysprosium containing silica particles was investigated by XPS analysis. The MRI contrast agent characteristics have been tested using RARE-T{sub 1} and RARE-T{sub 2} protocols. The contrast of MRI images delivered by the investigated samples was correlated with their local structure. Dysprosium disposal on microparticles with surface structure characterised by decreased connectivity of the silicate network units favours dark T{sub 2}-weighted MRI contrast properties.

  5. 40 CFR 145.31 - Approval process.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Approval process. 145.31 Section 145.31 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) STATE UIC PROGRAM REQUIREMENTS Program Approval, Revision and Withdrawal § 145.31 Approval process....

  6. 14 CFR 145.217 - Contract maintenance.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Contract maintenance. 145.217 Section 145...) SCHOOLS AND OTHER CERTIFICATED AGENCIES REPAIR STATIONS Operating Rules § 145.217 Contract maintenance. (a) A certificated repair station may contract a maintenance function pertaining to an article to...

  7. 21 CFR 161.145 - Canned oysters.

    Science.gov (United States)

    2010-04-01

    ... CONSUMPTION FISH AND SHELLFISH Requirements for Specific Standardized Fish and Shellfish § 161.145 Canned oysters. (a) Identity. (1) Canned oysters is the food prepared from one or any mixture of two or all of... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Canned oysters. 161.145 Section 161.145 Food...

  8. 50 CFR 648.145 - Possession limit.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Possession limit. 648.145 Section 648.145... Fishery § 648.145 Possession limit. (a) No person shall possess more than 25 black sea bass, in, or... that is not eligible for a black sea bass moratorium permit are subject to this possession limit....

  9. 49 CFR 192.145 - Valves.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Valves. 192.145 Section 192.145 Transportation... BY PIPELINE: MINIMUM FEDERAL SAFETY STANDARDS Design of Pipeline Components § 192.145 Valves. (a) Except for cast iron and plastic valves, each valve must meet the minimum requirements of API...

  10. 22 CFR 145.46 - Procurement records.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Procurement records. 145.46 Section 145.46... EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Procurement Standards § 145.46 Procurement records. Procurement records and files for purchases in excess of the small...

  11. 21 CFR 156.145 - Tomato juice.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Tomato juice. 156.145 Section 156.145 Food and... CONSUMPTION VEGETABLE JUICES Requirements for Specific Standardized Vegetable Juices § 156.145 Tomato juice. (a) Identity—(1) Definition. Tomato juice is the food intended for direct consumption, obtained...

  12. 17 CFR 1.45 - [Reserved

    Science.gov (United States)

    2010-04-01

    ... 17 Commodity and Securities Exchanges 1 2010-04-01 2010-04-01 false 1.45 Section 1.45 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION GENERAL REGULATIONS UNDER THE COMMODITY EXCHANGE ACT Miscellaneous § 1.45 ...

  13. 24 CFR 700.145 - Cost distribution.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Cost distribution. 700.145 Section 700.145 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... PUBLIC AND INDIAN HOUSING PROGRAMS) CONGREGATE HOUSING SERVICES PROGRAM § 700.145 Cost distribution....

  14. 31 CFR 14.5 - Certification.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Certification. 14.5 Section 14.5 Money and Finance: Treasury Office of the Secretary of the Treasury RIGHT TO FINANCIAL PRIVACY ACT § 14.5 Certification. Prior to obtaining the requested records pursuant to a formal written request,...

  15. 14 CFR 125.145 - Firewall construction.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Firewall construction. 125.145 Section 125.145 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED... Requirements § 125.145 Firewall construction. Each firewall and shroud must— (a) Be so made that no...

  16. Influence of exchange splitting on optical properties in gadolinium and dysprosium single crystals

    Energy Technology Data Exchange (ETDEWEB)

    Knyazev, Yu.V.; Bolotin, G.A. (AN SSSR, Sverdlovsk. Inst. Fiziki Metallov)

    1984-12-01

    The temperature dependences of optical conductivity in gadolinium and dysprosium single crystals at the light wave vector polarization along a hexagonal axis and in the basis plane are considered. A substantial anisotropy of interzonal absorption has been found. The sample transition into magnetically ordered state is shown to be accompanied by the emergence of resonance absorption peaks in the near infrared spectral region. The manifestation of these peculiarities is associated with quantum electron transitions between the s-, d-f- interaction-split energy bands near the Fermi level. Main peculiarities of the experimental spectrum of gadolinium optical conductivity found their reflection in theoretically calculated dispersion dependence.

  17. Therapeutic application of dysprosium-165-FHMA in the treatment of rheumatoid knee effusions

    Energy Technology Data Exchange (ETDEWEB)

    English, R.J.; Zalutsky, M.; Venkatesan, P.; Sledge, C.B.

    1986-03-01

    Radiation synovectomy utilizing a variety of radionuclides has proven to be an effective technique in the treatment of rheumatoid arthritis. The recent introduction of the short-lived radionuclide, Dysprosium-165 (/sup 165/Dy), as a replacement for the longer-lived radiocolloids has reduced nontarget dosimetry caused by leakage of the agent from the articular cavity. A review of the methods and status of radiation synovectomy, and the application of /sup 165/Dy-ferric hydroxide macroaggregates (FHMA) as an alternative therapeutic agent is described.

  18. Properties of dysprosium-doped gallium lanthanum sulfide fiber amplifiers operating at 1.3 microm.

    Science.gov (United States)

    Samson, B N; Schweizer, T; Hewak, D W; Laming, R I

    1997-05-15

    In light of recent progress in the fabrication of gallium lanthanum sulfide (GaLaS) fibers, we have modeled the performance of dysprosium-doped GaLaS fiber amplifiers operating at 1.3 microm . Based on experimental data, we find that the incorporation of a codopant (terbium) in the fiber core significantly shortens the optimum amplifier length from >30 m to approximately 3 m . Such a device may be practical, given the fiber losses currently achieved in GaLaS fibers.

  19. Effect of dysprosium on the kinetics and structural transformations during the decomposition of the supersaturated solid solution in magnesium-samarium alloys

    Science.gov (United States)

    Rokhlin, L. L.; Luk'yanova, E. A.; Tabachkova, N. Yu.; Dobatkina, T. V.; Tarytina, I. E.; Korol'kova, I. G.

    2017-03-01

    The effect of dysprosium added in the amounts such that it does not form an individual phase in equilibrium with solid magnesium on the decomposition of the supersaturated magnesium solid solution in Mg-Sm alloys is studied. The presence of dysprosium in Mg-Sm alloys is found to retard the decomposition of the supersaturated magnesium solid solution and to increase the hardening effect upon aging. When these alloys are aged, dysprosium is partly retained in the magnesium solid solution and partly enters into the compositions of the phases that form during the decomposition of the solid solution and are characteristic of Mg-Sm alloys.

  20. 19 CFR 145.23 - Time limits.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Time limits. 145.23 Section 145.23 Customs Duties... (CONTINUED) MAIL IMPORTATIONS Administrative Review of Mail Entries § 145.23 Time limits. A mail entry may be... requests such amendment within the time limits prescribed therein (see §§ 173.4 and 173.5 of this...

  1. Cyclic single-molecule magnets: from the odd-numbered heptanuclear to a dimer of heptanuclear dysprosium clusters.

    Science.gov (United States)

    Tian, Haiquan; Bao, Song-Song; Zheng, Li-Min

    2016-02-01

    A heptanuclear and a dimer of heptanuclear dysprosium clusters (Dy7 and Dy14) have been successfully synthesized by ingenious coalescence of the single and double pyrazinyl hydrazone as well as phosphonate ligands. The complexes feature the largest odd-numbered cyclic lanthanide clusters reported thus far. Both exhibit single molecule magnet behaviors at low temperature.

  2. A comparison of the effects of symmetry and magnetoanisotropy on paramagnetic relaxation in related dysprosium single ion magnets.

    Science.gov (United States)

    Williams, Ursula J; Mahoney, Brian D; DeGregorio, Patrick T; Carroll, Patrick J; Nakamaru-Ogiso, Eiko; Kikkawa, James M; Schelter, Eric J

    2012-06-07

    Dysprosium complexes of the tmtaa(2-) ligand were synthesized and characterized by X-band EPR and magnetism studies. Both complexes demonstrate magnetoanisotropy and slow paramagnetic relaxation. Comparison of these compounds with the seminal phthalocyanine complex [Dy(Pc)(2)](-) shows the azaannulide complexes are more susceptible to relaxation through non-thermal pathways.

  3. Dual responsive dysprosium-doped hydroxyapatite particles and toxicity reduction after functionalization with folic and glucuronic acids

    Energy Technology Data Exchange (ETDEWEB)

    Sánchez Lafarga, Ana Karen; Pacheco Moisés, Fermín P. [Departamento de Química, Universidad de Guadalajara, Marcelino García Barragán 1421, C.P. 44430, Guadalajara, Jalisco (Mexico); Gurinov, Andrey [Research Resources Center for Magnetic Resonance, Saint Petersburg State University, Universitetskij pr. 26, 198504 St. Petersburg (Russian Federation); Ortiz, Genaro Gabriel [Laboratorio Desarrollo-Envejecimiento, Enfermedades Neurodegenerativas, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano de Seguro Social (IMSS), Guadalajara, Jalisco (Mexico); Carbajal Arízaga, Gregorio Guadalupe, E-mail: gregoriocarbajal@yahoo.com.mx [Departamento de Química, Universidad de Guadalajara, Marcelino García Barragán 1421, C.P. 44430, Guadalajara, Jalisco (Mexico)

    2015-03-01

    The development of probes for biomedical applications demands materials with low toxicity levels besides fluorescence or magnetic properties to be detected by confocal microscopes or MRI resonators. Several drug delivery systems or other biomedical materials prepared with hydroxyapatite have been proposed, however, toxicity effects might arise when the size of particles is nanometric. In this study, hydroxyapatite functionalized with glucuronic or folic acids presented lower oxidative stress, measured from lipoperoxides and nitric oxide indicators in rats than pure hydroxyapatite. In separated experiments, hydroxyapatite was doped with dysprosium cations by coprecipitation producing a single crystal phase with fluorescent properties easily visualized by confocal microscopy when excited at 488 nm. These particles also presented the ability to modify the proton relaxation time in T1 maps collected by magnetic resonance imaging. These modified hydroxyapatite nanoparticles could be candidates to design bimodal probes with low toxicity. - Highlights: • Hydroxyapatite functionalized with glucuronic acid reduced oxidative stress in rats. • Functionalization with folic acid reduced oxidative stress in rats. • Dysprosium doping does not affect the crystalline structure of hydroxyapatite. • Dysprosium doped particles are visible in fluorescent microscope. • Dysprosium doped particles act as MRI contrast agents.

  4. Tuning Slow Magnetic Relaxation in a Two-Dimensional Dysprosium Layer Compound through Guest Molecules.

    Science.gov (United States)

    Chen, Qi; Li, Jian; Meng, Yin-Shan; Sun, Hao-Ling; Zhang, Yi-Quan; Sun, Jun-Liang; Gao, Song

    2016-08-15

    A novel two-dimensional dysprosium(III) complex, [Dy(L)(CH3COO)]·0.5DMF·H2O·2CH3OH (1), has been successfully synthesized from a new pyridine-N-oxide (PNO)-containing ligand, namely, N'-(2-hydroxy-3-methoxybenzylidene)pyridine-N-oxidecarbohydrazide (H2L). Single-crystal X-ray diffraction studies reveal that complex 1 is composed of a dinuclear dysprosium subunit, which is further extended by the PNO part of the ligand to form a two-dimensional layer. Magnetic studies indicate that complex 1 shows well-defined temperature- and frequency-dependent signals under a zero direct-current (dc) field, typical of slow magnetic relaxation with an effective energy barrier Ueff of 33.6 K under a zero dc field. Interestingly, powder X-ray diffraction and thermogravimetric analysis reveal that compound 1 undergoes a reversible phase transition that is induced by the desorption and absorption of methanol and water molecules. Moreover, the desolvated sample [Dy(L)(CH3COO)]·0.5DMF (1a) also exhibits slow magnetic relaxation but with a higher anisotropic barrier of 42.0 K, indicating the tuning effect of solvent molecules on slow magnetic relaxation.

  5. Dosimetric properties of dysprosium doped calcium magnesium borate glass subjected to Co-60 gamma ray

    Energy Technology Data Exchange (ETDEWEB)

    Omar, R. S., E-mail: ratnasuffhiyanni@gmail.com; Wagiran, H., E-mail: husin@utm.my; Saeed, M. A. [Department of Physics, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru (Malaysia)

    2016-01-22

    Thermoluminescence (TL) dosimetric properties of dysprosium doped calcium magnesium borate (CMB:Dy) glass are presented. This study is deemed to understand the application of calcium as the modifier in magnesium borate glass with the presence of dysprosium as the activator to be performed as TL dosimeter (TLD). The study provides fundamental knowledge of a glass system that may lead to perform new TL glass dosimetry application in future research. Calcium magnesium borate glass systems of (70-y) B{sub 2}O{sub 3} − 20 CaO – 10 MgO-(y) Dy{sub 2}O{sub 3} with 0.05  mol % ≤ y ≤ 0.7  mol % of dyprosium were prepared by melt-quenching technique. The amorphous structure and TL properties of the prepared samples were determined using powder X-ray diffraction (XRD) and TL reader; model Harshaw 4500 respectively. The samples were irradiated to Co-60 gamma source at a dose of 50 Gy. Dosimetric properties such as annealing procedure, time temperature profile (TTP) setting, optimization of Dy{sub 2}O{sub 3} concentration of 0.5 mol % were determined for thermoluminescence dosimeter (TLD) reader used.

  6. 14 CFR 145.101 - General.

    Science.gov (United States)

    2010-01-01

    ... OTHER CERTIFICATED AGENCIES REPAIR STATIONS Housing, Facilities, Equipment, Materials, and Data § 145.101 General. A certificated repair station must provide housing, facilities, equipment, materials, and...

  7. 14 CFR 145.59 - Ratings.

    Science.gov (United States)

    2010-01-01

    ... operation, and a generator, including starters, voltage regulators, electric motors, electrically driven... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Ratings. 145.59 Section 145.59 Aeronautics..., electrical or electronic intercrew signaling devices, and similar equipment. This equipment does not...

  8. 19 CFR 145.25 - Entry correct.

    Science.gov (United States)

    2010-04-01

    ... director believes the duty originally assessed was correct, he shall send the addressee a notice in writing... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry correct. 145.25 Section 145.25 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE...

  9. 22 CFR 145.43 - Competition.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Competition. 145.43 Section 145.43 Foreign... Competition. All procurement transactions shall be conducted in a manner to provide, to the maximum extent practical, open and free competition. The recipient shall be alert to organizational conflicts of...

  10. 32 CFR 536.145 - Solatia payment.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Solatia payment. 536.145 Section 536.145 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY CLAIMS AND ACCOUNTS CLAIMS AGAINST THE... States of Micronesia, Japan, Korea, and Thailand. In other countries, the FCC should consult the...

  11. 22 CFR 145.24 - Program income.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Program income. 145.24 Section 145.24 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS GRANTS AND AGREEMENTS WITH INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management §...

  12. 9 CFR 145.11 - Supervision.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Supervision. 145.11 Section 145.11 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... Supervision. (a) The Official State Agency may designate qualified persons as Authorized Agents to do...

  13. 22 CFR 145.52 - Financial reporting.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Financial reporting. 145.52 Section 145.52....52 Financial reporting. (a) The following forms or such other forms as may be approved by OMB are authorized for obtaining financial information from recipients. (1) SF-269 or SF-269A, Financial...

  14. 37 CFR 1.45 - Joint inventors.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Joint inventors. 1.45 Section... Patent § 1.45 Joint inventors. (a) Joint inventors must apply for a patent jointly and each must make the... patent for an invention invented by them jointly, except as provided in § 1.47. (b) Inventors may...

  15. 21 CFR 172.145 - Heptylparaben.

    Science.gov (United States)

    2010-04-01

    ... noncarbonated soft drinks and fruit-based beverages in amounts not to exceed 20 parts per million, when... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Heptylparaben. 172.145 Section 172.145 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR...

  16. 22 CFR 145.48 - Contract clauses.

    Science.gov (United States)

    2010-04-01

    ... breaches the contract terms, and provide for such remedial actions as may be appropriate. (b) All contracts... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Contract clauses. 145.48 Section 145.48 Foreign... Contract clauses. The recipient shall include, in addition to clauses to define a sound and...

  17. Luminescent properties of dysprosium(Ⅲ) ions in LaAlO3 nanocrystallites

    Institute of Scientific and Technical Information of China (English)

    K. Lema(n)ski; P.J. Dere(n)

    2011-01-01

    The absorption and emission spectra as well as decay time profile of Dy3+ ions in LaAlO3 nanocrystals were analyzed.The crystal structure of LaAlO3 was confirmed from XRD measurement.The emission peaks from blue to red came from main emitting level of dysprosium 4F9/2 to the ground and other excited levels of Dy3+ ions.Cross relaxation process led to non-radiative quenching of luminescence,so that the lifetime of the 4F9/2 energy level ions decreased with increasing amount of doped Dy3+ ions.The cross relaxation transfer rates were experimentally determined as a function of Dy3+ concentration.

  18. Magnetic ordering temperatures in rare earth metal dysprosium under ultrahigh pressures

    Science.gov (United States)

    Samudrala, Gopi K.; Tsoi, Georgiy M.; Weir, Samuel T.; Vohra, Yogesh K.

    2014-04-01

    Magnetic ordering temperatures in heavy rare earth metal dysprosium (Dy) have been studied using an ultrasensitive electrical transport measurement technique in a designer diamond anvil cell to a pressure of 69 GPa and a temperature of 10 K. Previous studies using magnetic susceptibility measurements at high pressures were able to track magnetic ordering temperature only till 7 GPa in the hexagonal close packed (hcp) phase of Dy. Our studies indicate that the magnetic ordering temperature shows an abrupt drop of 80 K at the hcp-Sm phase transition followed by a gradual decrease that continues till 17 GPa. This is followed by a rapid increase in the magnetic ordering temperatures in the double hcp phase and finally leveling off in the distorted face centered cubic phase of Dy. Our studies reaffirm that 4f-shell remains localized in Dy and there is no loss of magnetic moment or 4f-shell delocalization for pressures up to 69 GPa.

  19. Electrochemical behaviour of dysprosium in the eutectic LiCl-KCl at W and Al electrodes

    Energy Technology Data Exchange (ETDEWEB)

    Castrillejo, Y. [Dpto de Quimica Analitica, Facultad de Ciencias, Universidad de Valladolid, Prado de la Magdalena s/n, 47005 Valladolid (Spain)]. E-mail: ycastril@qa.uva.es; Bermejo, M.R. [Dpto de Quimica Analitica, Facultad de Ciencias, Universidad de Valladolid, Prado de la Magdalena s/n, 47005 Valladolid (Spain); Barrado, A.I. [Dpto de Quimica Analitica, Facultad de Ciencias, Universidad de Valladolid, Prado de la Magdalena s/n, 47005 Valladolid (Spain); Pardo, R. [Dpto de Quimica Analitica, Facultad de Ciencias, Universidad de Valladolid, Prado de la Magdalena s/n, 47005 Valladolid (Spain); Barrado, E. [Dpto de Quimica Analitica, Facultad de Ciencias, Universidad de Valladolid, Prado de la Magdalena s/n, 47005 Valladolid (Spain); Martinez, A.M. [Department of Materials Technology, Sem Saelands vei 6, 7491 Trondheim (Norway)

    2005-03-15

    The electrochemical behaviour of DyCl{sub 3} was studied in the eutectic LiCl-KCl at different temperatures. The cathodic reaction can be written:Dy(III)+3e-bar Dy(0)which can be divided in two very close cathodic steps:Dy(III)+1e-bar Dy(II)andDy(II)+2e-bar Dy(0)Transient electrochemical techniques, such as cyclic voltammetry, chronopotentiometry, and chronoamperometry were used in order to study the reaction mechanism and the transport parameters of electroactive species at a tungsten electrode. The results showed that in the eutectic LiCl-KCl, electrocrystallization of dysprosium seems to be the controlling electrochemical step. Chronoamperometric studies indicated instantaneous nucleation of dysprosium with three dimensional growth of the nuclei whatever the applied overpotential.Mass transport towards the electrode is a simple diffusion process, and the diffusion coefficient of the electroactive species, i.e. Dy(III), has been calculated. The validity of the Arrhenius law was also verified by plotting the variation of the logarithm of the diffusion coefficient versus 1/T.In addition, the electrode reactions of the LiCl-KCl-DyCl{sub 3} solutions at an Al wire were also investigated by cyclic voltammetry and open circuit chronopotentiometry. The redox potential of the Dy(III)/Dy couple at the Al electrode was observed at more positive potentials values than those at the inert electrode. This potential shift was thermodynamically analyzed by a lowering of activity of Dy in the metal phase due to the formation of intermetallic compounds.

  20. 7 CFR 1260.145 - Appointment.

    Science.gov (United States)

    2010-01-01

    ... Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE BEEF PROMOTION AND RESEARCH Beef Promotion and Research Order Cattlemen's Beef Promotion and Research Board § 1260.145 Appointment. (a)...

  1. 32 CFR 145.3 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... COOPERATION WITH THE OFFICE OF SPECIAL COUNSEL OF THE MERIT SYSTEMS PROTECTION BOARD § 145.3 Definitions.... (i) A decision concerning pay, benefits, or awards, or concerning education or training if...

  2. Structural, optical, thermal, mechanical and dielectric studies of Sulfamic acid single crystals: An influence of dysprosium (Dy3+) doping

    Science.gov (United States)

    Singh, Budhendra; Shkir, Mohd.; AlFaify, S.; Kaushal, Ajay; Nasani, Narendar; Bdikin, Igor; Shoukry, H.; Yahia, I. S.; Algarni, H.

    2016-09-01

    Sulfamic acid is a potential material that exhibits excellent optical properties. A good quality, pure and dysprosium (Dy3+) doped (2.5 and 5 mol %) Sulfamic acid (SA) single crystals were grown successfully by slow cooling method. Structural study revealed a slight change in its lattice parameters and volume, suggesting the successful incorporation of Dy3+ in crystal system. The existence of dysprosium in the system was also confirmed. Presence of various vibrational modes was confirmed. Optical transparency was found to have a significant effect with variation in the doping concentration. Furthermore, a marked enhancement in its mechanical parameters with doping was also identified by nanoindentation technique. Etching study was also performed on the grown crystals to study the etch-pit formation and growth mechanism. Effect of doping on the thermal stability was analysed. All the results were compared and discussed in detail to get insight of the effect of doping concentration on Sulfamic acid crystal.

  3. Optical trapping of ultracold dysprosium atoms: transition probabilities, dynamic dipole polarizabilities and van der Waals $C_6$ coefficients

    CERN Document Server

    Li, Hui; Dulieu, Olivier; Nascimbene, Sylvain; Lepers, Maxence

    2016-01-01

    The efficiency of optical trapping of ultracold atoms depend on the atomic dynamic dipole polarizability governing the atom-field interaction. In this article, we have calculated the real and imaginary parts of the dynamic dipole polarizability of dysprosium in the ground and first excited level. Due to the high electronic angular momentum of those two states, the polarizabilities possess scalar, vector and tensor contributions that we have computed, on a wide range of trapping wavelengths, using the sum-over-state formula. Using the same formalism, we have also calculated the $C_6$ coefficients characterizing the van der Waals interaction between two dysprosium atoms in the two lowest levels. We have computed the energies of excited states and the transition probabilities appearing in the sums, using a combination of \\textit{ab initio} and least-square-fitting techniques provided by the Cowan codes and extended in our group. Regarding the real part of the polarizability, for field frequencies far from atomic...

  4. Mixed (phthalocyaninato)(Schiff-base) di-dysprosium sandwich complexes. Effect of magnetic coupling on the SMM behavior.

    Science.gov (United States)

    Wang, Hailong; Liu, Chenxi; Liu, Tao; Zeng, Suyuan; Cao, Wei; Ma, Qi; Duan, Chunying; Dou, Jianmin; Jiang, Jianzhuang

    2013-11-21

    Reaction between Schiff-base ligand and half-sandwich complex M(Pc)(acac) led to the isolation of new sandwich-type mixed (phthalocyaninato)(Schiff-base) di-lanthanide compounds M2(Pc)2(L)H2O (M = Dy, Gd) (1, 2) [H2Pc = metal free phthalocyanine, Hacac = acetylacetone, H2L = N,N'-bis(3-methyloxysalicylidene)benzene-1,2-diamine] with the triple-decker molecular structure clearly revealed by single crystal X-ray diffraction analysis. For the comparative studies, sandwich triple-decker analogues with pure Schiff-base ligand M2(L)3H2O (M = Dy, Gd) (3, 4) were also prepared. Dynamic magnetic measurement result reveals the single-molecule magnet (SMM) nature of the di-dysprosium derivative 1, while the static magnetic investigation over both pure and the diamagnetic diluted samples of this compound discloses the interionic ferromagnetic coupling between the two dysprosium ions, which in turn effectively suppresses the QTM and enhances the energy barrier of this SMM. Nevertheless, comparative studies over the static magnetic properties of the di-dysprosium triple-decker complexes 1 and 3 indicate the stronger magnetic coupling between the two lanthanide ions in mixed (phthalocyaninato)(Schiff-base) species than in the pure Schiff-base triple-decker analogue, suggesting the special coordination sphere around the dysprosium ions in the former compound over the latter one on the more intense inter-ionic ferromagnetic coupling. As a very small step towards understanding the structure-property relationship, the present result will be surely helpful for the design and synthesis of the multinuclear lanthanide-based SMMs with good properties.

  5. Evaluating United States and world consumption of neodymium, dysprosium, terbium, and praseodymium in final products

    Science.gov (United States)

    Hart, Matthew

    This paper develops scenarios of future rare-earth-magnet metal (neodymium, dysprosium, terbium, and praseodymium) consumption in the permanent magnets used in wind turbines and hybrid electric vehicles. The scenarios start with naive base-case scenarios for growth in wind-turbine and hybrid-electric-vehicle sales over the period 2011 to 2020, using historical data for each good. These naive scenarios assume that future growth follows time trends in historical data and does not depend on any exogenous variable. Specifically, growth of each technological market follows historical time trends, and the amount of rare earths used per unit of technology remains fixed. The chosen reference year is 2010. Implied consumptions of the rare earth magnet metals are calculated from these scenarios. Assumptions are made for the material composition of permanent magnets, the market share of permanent-magnet wind turbines and vehicles, and magnet weight per unit of technology. Different scenarios estimate how changes in factors like the material composition of magnets, growth of the economy, and the price of a substitute could affect future consumption. Each scenario presents a different method for reducing rare earth consumption and could be interpreted as potential policy choices. In 2010, the consumption (metric tons, rare-earth-oxide equivalent) of each rare-earth-magnet metal was as follows. Total neodymium consumption in the world for both technologies was 995 tons; dysprosium consumption was 133 tons; terbium consumption was 50 tons; praseodymium consumption was zero tons. The base scenario for wind turbines shows there could be strong, exponential growth in the global wind turbine market. New U.S. sales of hybrid vehicles would decline (in line with the current economic recession) while non-U.S. sales increase through 2020. There would be an overall increase in the total amount of magnetic rare earths consumed in the world. Total consumption of each rare earth in the short

  6. Coexistence of symmetric and asymmetric shapes in $^{145}$/Ba, $^{145}$/La

    CERN Document Server

    Hamilton, J H; Jones, E F; Ramayya, A V; Hwang, J K; Gore, P M; Wang, M G; Cole, J D; Collins, W E; Peker, L K

    2000-01-01

    A new region of stable octupole deformation was predicted to occur with its center around the reinforcing shell gaps for beta /sub 3 /~0.15 at Z=56, N=88, and with /sub 56//sup 145/Ba/sub 89/ was predicted to be a prime candidate for stable octupole deformation. Evidence for stable octupole deformation was found in /sup 144/Ba, /sup 146/Ce and expanded to include odd-A /sup 143/Ba and other neighboring isotopes but was not observed in /sup 145/Ba. Recently we found evidence for the rotational enhancement of stable octupole deformation in /sup 145,147/La. In a reinvestigation of /sup 145/Ba, two new bands were discovered that are connected by enhanced, intertwined E1 transitions to two different previously known bands in /sup 145/Ba. These new data support the predicted presence of octupole deformation in /sup 145/Ba, which is rotation-enhanced above about spin 19/2. In both /sup 145/La and /sup 145/Ba, the low spin ground bands are built on a symmetric rotor shape and at intermediate spins there are shifts to...

  7. Luminescence features of dysprosium and phosphorus oxide co-doped lithium magnesium borate glass

    Science.gov (United States)

    Hashim, S.; Mhareb, M. H. A.; Ghoshal, S. K.; Alajerami, Y. S. M.; Saripan, M. I.; Bradley, D. A.

    2017-08-01

    Lithium magnesium borate (LMB) glass system co-doped with the oxides of dysprosium (Dy2O3) and phosphorus (P2O5) were synthesized using melt-quenching method. Prepared samples were characterized using various techniques to determine the effects of co-dopants concentration variation on their thermoluminescence (TL) and photoluminescence (PL) properties. TL glow curves of LMB:0.5Dy sample revealed a single prominent peak at Tm=190 °C, where TL intensity was enhanced by a factor of 2.5 with the increase of P2O5 concentration up to 1 mol%. This enhancement was accompanied by a shift in Tm towards higher temperature. Good linearity in the range of 1-100 Gy with linear correlation coefficient of 0.998 was achieved. PL spectra displayed two significant peaks centred at 481 nm and 573 nm. These attractive luminescence features of the proposed glass system may be useful for the development of radiation dosimetry.

  8. Single-molecule magnet behavior for an antiferromagnetically superexchange-coupled dinuclear dysprosium(III) complex.

    Science.gov (United States)

    Long, Jérôme; Habib, Fatemah; Lin, Po-Heng; Korobkov, Ilia; Enright, Gary; Ungur, Liviu; Wernsdorfer, Wolfgang; Chibotaru, Liviu F; Murugesu, Muralee

    2011-04-13

    A family of five dinuclear lanthanide complexes has been synthesized with general formula [Ln(III)(2)(valdien)(2)(NO(3))(2)] where (H(2)valdien = N1,N3-bis(3-methoxysalicylidene)diethylenetriamine) and Ln(III) = Eu(III)1, Gd(III)2, Tb(III)3, Dy(III)4, and Ho(III)5. The magnetic investigations reveal that 4 exhibits single-molecule magnet (SMM) behavior with an anisotropic barrier U(eff) = 76 K. The step-like features in the hysteresis loops observed for 4 reveal an antiferromagnetic exchange coupling between the two dysprosium ions. Ab initio calculations confirm the weak antiferromagnetic interaction with an exchange constant J(Dy-Dy) = -0.21 cm(-1). The observed steps in the hysteresis loops correspond to a weakly coupled system similar to exchange-biased SMMs. The Dy(2) complex is an ideal candidate for the elucidation of slow relaxation of the magnetization mechanism seen in lanthanide systems.

  9. Thermoluminescence properties of lithium magnesium borate glasses system doped with dysprosium oxide.

    Science.gov (United States)

    Mhareb, M H A; Hashim, S; Ghoshal, S K; Alajerami, Y S M; Saleh, M A; Razak, N A B; Azizan, S A B

    2015-12-01

    We report the impact of dysprosium (Dy(3+)) dopant and magnesium oxide (MgO) modifier on the thermoluminescent properties of lithium borate (LB) glass via two procedures. The thermoluminescence (TL) glow curves reveal a single prominent peak at 190 °C for 0.5 mol% of Dy(3+). An increase in MgO contents by 10 mol% enhances the TL intensity by a factor of 1.5 times without causing any shift in the maximum temperature. This enhancement is attributed to the occurrence of extra electron traps created via magnesium and the energy transfer to trivalent Dy(3+) ions. Good linearity in the range of 0.01-4 Gy with a linear correlation coefficient of 0.998, fading as low as 21% over a period of 3 months, excellent reproducibility without oven annealing and tissue equivalent effective atomic numbers ~8.71 are achieved. The trap parameters, including geometric factor (μg), activation energy (E) and frequency factor (s) associated with LMB:Dy are also determined. These favorable TL characteristics of prepared glasses may contribute towards the development of Li2O-MgO-B2O3 radiation dosimeters.

  10. Optical properties of zinc borotellurite glass doped with trivalent dysprosium ion

    Science.gov (United States)

    Ami Hazlin, M. N.; Halimah, M. K.; Muhammad, F. D.; Faznny, M. F.

    2017-04-01

    The zinc borotellurite doped with dysprosium oxide glass samples with chemical formula {[(TeO2) 0 . 7(B2O3) 0 . 3 ] 0 . 7(ZnO) 0 . 3 } 1 - x(Dy2O3)x (where x=0.01, 0.02, 0.03, 0.04 and 0.05 M fraction) were prepared by using conventional melt quenching technique. The structural and optical properties of the proposed glass systems were characterized by using X-ray diffraction (XRD) spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, and UV-VIS spectroscopy. The amorphous nature of the glass systems is confirmed by using XRD technique. The infrared spectra of the glass systems indicate three obvious absorption bands which are assigned to BO3 and TeO4 vibrational groups. Based on the absorption spectra obtained, the direct and indirect optical band gaps, as well as the Urbach energy were calculated. It is observed that both the direct and indirect optical band gaps increase with the concentration of Dy3+ ions. On the other hand, the Urbach energy is observed to decrease as the concentration of Dy3+ ions increases.

  11. Isolation of {sup 163}Ho from dysprosium target material by HPLC for neutrino mass measurements

    Energy Technology Data Exchange (ETDEWEB)

    Mocko, Veronika; Taylor, Wayne A.; Nortier, Francois M.; Engle, Jonathan W.; Pollington, Anthony D.; Kunde, Gerd J.; Rabin, Michael W.; Birnbaum, Eva R. [Los Alamos National Laboratory, Los Alamos, NM (United States). Chemistry Div.; Barnhart, Todd E.; Nickles, Robert J. [Univ. Wisconsinn, Madison, WI (United States). Dept. of Medical Physics

    2015-07-01

    The rare earth isotope {sup 163}Ho is of interest for neutrino mass measurements. This report describes the isolation of {sup 163}Ho from a proton-irradiated dysprosium target and its purification. A Dy metal target was irradiated with 16 MeV protons for 10 h. After target dissolution, {sup 163}Ho was separated from the bulk Dy via cation-exchange high performance liquid chromatography using 70 mmol dm{sup -3} α-hydroxyisobutyric acid as the mobile phase. Subsequent purification of the collected Ho fraction was performed to remove the α-hydroxyisobutyrate chelating agent and to concentrate the Ho in a low ionic strength aqueous matrix. The final solution was characterized by MC-ICP-MS to determine the {sup 163}Ho/{sup 165}Ho ratio, {sup 163}Ho and the residual Dy content. The HPLC purification process resulted in a decontamination factor 1.4 x 10{sup 5} for Dy. The isolated Ho fraction contained 24.8 ± 1.3 ng of {sup 163}Ho corresponding to holmium recovery of 72 ± 3%.

  12. 9 CFR 145.81 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Primary Meat-Type Chicken Breeding Flocks and Products § 145.81 Definitions. Except where the context otherwise...: Chicks. Newly hatched chickens. Primary meat-type chicken breeding flocks. Foundation flocks that are...

  13. 9 CFR 145.82 - Participation.

    Science.gov (United States)

    2010-01-01

    ... LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Primary Meat-Type Chicken Breeding Flocks and Products § 145.82 Participation. Participating flocks of primary meat-type chickens, and the eggs and chicks produced from them, shall comply with the applicable...

  14. 9 CFR 145.71 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Primary Egg-Type Chicken Breeding Flocks and Products § 145.71 Definitions. Except where the context otherwise...: Chicks. Newly hatched chickens. Primary egg-type chicken breeding flocks. Foundation flocks that are...

  15. 9 CFR 145.31 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Multiplier Meat-Type Chicken Breeding Flocks and Products § 145.31 Definitions. Except where the context..., to mean: Chicks. Newly hatched chickens. Meat type chicken breeding flocks. Flocks that are composed...

  16. 9 CFR 145.21 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Multiplier Egg-Type Chicken Breeding Flocks and Products § 145.21 Definitions. Except where the context..., to mean: Chicks. Newly hatched chickens. Egg type chicken breeding flocks. Flocks that are composed...

  17. 9 CFR 145.22 - Participation.

    Science.gov (United States)

    2010-01-01

    ... LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Multiplier Egg-Type Chicken Breeding Flocks and Products § 145.22 Participation. Participating flocks of multiplier egg type chickens, and the eggs and chicks produced from them, shall comply with the applicable...

  18. 9 CFR 145.32 - Participation.

    Science.gov (United States)

    2010-01-01

    ... LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Multiplier Meat-Type Chicken Breeding Flocks and Products § 145.32 Participation. Participating flocks of multiplier meat type chickens, and the eggs and chicks produced from them, shall comply with the applicable...

  19. 9 CFR 145.14 - Testing.

    Science.gov (United States)

    2010-01-01

    ... IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY General Provisions § 145.14 Testing... birds from meat-type chicken, waterfowl, exhibition poultry, and game bird flocks must be the same as..., the flockowner, and the Administrator. If the State Inspector determines that a primary breeding flock...

  20. 9 CFR 145.72 - Participation.

    Science.gov (United States)

    2010-01-01

    ... LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Primary Egg-Type Chicken Breeding Flocks and Products § 145.72 Participation. Participating flocks of primary egg-type chickens, and the eggs and chicks produced from them, shall comply with the applicable...

  1. 22 CFR 145.27 - Allowable costs.

    Science.gov (United States)

    2010-04-01

    ... Relations DEPARTMENT OF STATE CIVIL RIGHTS GRANTS AND AGREEMENTS WITH INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management § 145...-Profit Organizations.” The allowability of costs incurred by institutions of higher education...

  2. 22 CFR 145.22 - Payment.

    Science.gov (United States)

    2010-04-01

    ... Relations DEPARTMENT OF STATE CIVIL RIGHTS GRANTS AND AGREEMENTS WITH INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management § 145... financial management systems that meet the standards for fund control and accountability as established...

  3. 21 CFR 145.120 - Canned berries.

    Science.gov (United States)

    2010-04-01

    ... Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION CANNED FRUITS Requirements for Specific Standardized Canned Fruits § 145.120 Canned berries. (a... section are prepared from stemmed fruit of the following optional varietal types of berry...

  4. 21 CFR 145.175 - Canned pears.

    Science.gov (United States)

    2010-04-01

    ... optional ingredients. (i) Natural and artificial flavors. (ii) Spice. (iii) Vinegar, lemon juice, or organic acids. (iv) Artificial colors. Such food is sealed in a container and before or after sealing is... sweetener(s) may be added. Sweeteners defined in § 145.3 shall be as defined therein, except that...

  5. 9 CFR 145.52 - Participation.

    Science.gov (United States)

    2010-01-01

    ... Waterfowl, Exhibition Poultry, and Game Bird Breeding Flocks and Products § 145.52 Participation. Participating flocks of waterfowl, exhibition poultry, and game birds, and the eggs and baby poultry produced...-3I”, printed or stamped on the invoice; (2) The hatchery name and address; (3) The date of shipment...

  6. Acute dysprosium toxicity to Daphnia pulex and Hyalella azteca and development of the biotic ligand approach.

    Science.gov (United States)

    Vukov, Oliver; Smith, D Scott; McGeer, James C

    2016-01-01

    The toxicological understanding of rare earth elements (REEs) in the aquatic environment is very limited but of increasing concern. The objective of this research is to compare the toxicological effect of the REE dysprosium to the freshwater invertebrates Daphnia pulex and Hyalella azteca and in the more sensitive organism, understand the toxicity modifying influence of Ca, Na, Mg, pH and dissolved organic matter (DOM). Standard methods (Environment Canada) were followed for testing and culture in media of intermediate hardness (60mg CaCO3 mg/L) at pH 7.8 with Ca at 0.5, Na 0.5, Mg 0.125 (mM) and 23°C. Acute toxicity tests were done with azteca and D. pulex revealed Hyalella to be 1.4 times more sensitive than Daphnia. Additions of Ca and Na but not Mg provided significant protection against Dy toxicity to Hyalella. Similarly, low pH was associated with reduction in toxicity. Exposures which were pH buffered with and without MOPS were significantly different and indicated that MOPS enhanced Dy toxicity. DOM also mitigated Dy toxicity. Biotic ligand based parameters (LogK values) were calculated based on free ion relationships as determined by geochemical equilibrium modeling software (WHAM ver. 7.02). The logK value for Dy(3+) toxicity to Hyalella was 7.75 while the protective influence of Ca and Na were 3.95 and 4.10, respectively. This study contributes data towards the development of site specific water quality guidelines and criteria for Dy and possibly REEs in general and offers insight into the complex bio-geochemical nature of this element.

  7. Optical trapping of ultracold dysprosium atoms: transition probabilities, dynamic dipole polarizabilities and van der Waals C 6 coefficients

    Science.gov (United States)

    Li, H.; Wyart, J.-F.; Dulieu, O.; Nascimbène, S.; Lepers, M.

    2017-01-01

    The efficiency of the optical trapping of ultracold atoms depends on the atomic dynamic dipole polarizability governing the atom-field interaction. In this article, we have calculated the real and imaginary parts of the dynamic dipole polarizability of dysprosium in the ground and first excited levels. Due to the high electronic angular momentum of those two states, the polarizabilities possess scalar, vector and tensor contributions that we have computed, on a wide range of trapping wavelengths, using the sum-over-state formula. Using the same formalism, we have also calculated the C 6 coefficients characterizing the van der Waals interaction between two dysprosium atoms in the two lowest levels. We have computed the energies of excited states and the transition probabilities appearing in the sums, using a combination of ab initio and least-square-fitting techniques provided by the Cowan codes and extended in our group. Regarding the real part of the polarizability, for field frequencies far from atomic resonances, the vector and tensor contributions are two-orders-of-magnitude smaller than the scalar contribution, whereas for the imaginary part, the vector and tensor contributions represent a noticeable fraction of the scalar contribution. Finally, our anisotropic C 6 coefficients are much smaller than those published in the literature.

  8. 49 CFR 37.145 - State comment on plans.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false State comment on plans. 37.145 Section 37.145 Transportation Office of the Secretary of Transportation TRANSPORTATION SERVICES FOR INDIVIDUALS WITH DISABILITIES (ADA) Paratransit as a Complement to Fixed Route Service § 37.145 State comment on plans....

  9. 14 CFR 145.211 - Quality control system.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Quality control system. 145.211 Section 145...) SCHOOLS AND OTHER CERTIFICATED AGENCIES REPAIR STATIONS Operating Rules § 145.211 Quality control system. (a) A certificated repair station must establish and maintain a quality control system acceptable...

  10. 19 CFR 145.2 - Mail subject to Customs examination.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Mail subject to Customs examination. 145.2 Section 145.2 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) MAIL IMPORTATIONS General Provisions § 145.2 Mail subject to...

  11. 22 CFR 145.37 - Property trust relationship.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Property trust relationship. 145.37 Section 145.37 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS GRANTS AND AGREEMENTS WITH INSTITUTIONS OF... Standards § 145.37 Property trust relationship. Real property, equipment, intangible property and debt...

  12. 9 CFR 145.44 - Terminology and classification; States.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Terminology and classification; States. 145.44 Section 145.44 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... Special Provisions for Turkey Breeding Flocks and Products § 145.44 Terminology and classification;...

  13. 26 CFR 145.4061-1 - Application to manufacturers tax.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 17 2010-04-01 2010-04-01 false Application to manufacturers tax. 145.4061-1...) MISCELLANEOUS EXCISE TAXES (CONTINUED) TEMPORARY EXCISE TAX REGULATIONS UNDER THE HIGHWAY REVENUE ACT OF 1982 (PUB. L. 97-424) § 145.4061-1 Application to manufacturers tax. The provisions of § 145.4051-1(e)...

  14. 10 CFR 1.45 - Office of Nuclear Regulatory Research.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Office of Nuclear Regulatory Research. 1.45 Section 1.45... Program Offices § 1.45 Office of Nuclear Regulatory Research. The Office of Nuclear Regulatory Research— (a) Plans, recommends, and implements programs of nuclear regulatory research, standards development...

  15. 14 CFR 145.207 - Repair station manual.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Repair station manual. 145.207 Section 145...) SCHOOLS AND OTHER CERTIFICATED AGENCIES REPAIR STATIONS Operating Rules § 145.207 Repair station manual. (a) A certificated repair station must prepare and follow a repair station manual acceptable to...

  16. Acute dysprosium toxicity to Daphnia pulex and Hyalella azteca and development of the biotic ligand approach

    Energy Technology Data Exchange (ETDEWEB)

    Vukov, Oliver, E-mail: vuko3930@mylaurier.ca [Biology Department, Wilfrid Laurier University, Waterloo, Ontario N2L 3C5 (Canada); Smith, D. Scott [Chemistry Department, Wilfrid Laurier University, Waterloo, Ontario N2L 3C5 (Canada); McGeer, James C. [Biology Department, Wilfrid Laurier University, Waterloo, Ontario N2L 3C5 (Canada)

    2016-01-15

    The toxicological understanding of rare earth elements (REEs) in the aquatic environment is very limited but of increasing concern. The objective of this research is to compare the toxicological effect of the REE dysprosium to the freshwater invertebrates Daphnia pulex and Hyalella azteca and in the more sensitive organism, understand the toxicity modifying influence of Ca, Na, Mg, pH and dissolved organic matter (DOM). Standard methods (Environment Canada) were followed for testing and culture in media of intermediate hardness (60 mg CaCO{sub 3} mg/L) at pH 7.8 with Ca at 0.5, Na 0.5, Mg 0.125 (mM) and 23 °C. Acute toxicity tests were done with <24 h old neonates for 48 h in the case of D. pulex and with 2–9 days old offspring for 96 h tests with Hyalella. The potential protective effect of cationic competition was tested with Ca (0.5–2.0 mM), Na (0.5–2.0 mM) and Mg (0.125–0.5 mM). The effect of pH (6.5–8.0) and Suwannee River DOM complexation (at dissolved organic carbon (DOC) concentrations of 9 and 13 mg C/L) were evaluated. Dissolved Dy concentrations were lower than total (unfiltered) indicating precipitation, particularly at higher concentrations. Acute toxicity of Dy to H. azteca and D. pulex revealed Hyalella to be 1.4 times more sensitive than Daphnia. Additions of Ca and Na but not Mg provided significant protection against Dy toxicity to Hyalella. Similarly, low pH was associated with reduction in toxicity. Exposures which were pH buffered with and without MOPS were significantly different and indicated that MOPS enhanced Dy toxicity. DOM also mitigated Dy toxicity. Biotic ligand based parameters (Log K values) were calculated based on free ion relationships as determined by geochemical equilibrium modeling software (WHAM ver. 7.02). The log K value for Dy{sup 3+} toxicity to Hyalella was 7.75 while the protective influence of Ca and Na were 3.95 and 4.10, respectively. This study contributes data towards the development of site specific

  17. White light emission of dysprosium doped lanthanum calcium phosphate oxide and oxyfluoride glasses

    Science.gov (United States)

    Luewarasirikul, N.; Kim, H. J.; Meejitpaisan, P.; Kaewkhao, J.

    2017-04-01

    Lanthanum calcium phosphate oxide and oxyfluoride glasses doped with dysprosium oxide were prepared by melt-quenching technique with chemical composition 20La2O3:10CaO:69P2O5:1Dy2O3 and 20La2O3:10CaF2:69P2O5:1Dy2O3. The physical, optical and luminescence properties of the glass samples were studied to evaluate their potential to using as luminescence materials for solid-state lighting applications. The density, molar volume and refractive index of the glass samples were carried out. The optical and luminescence properties were studied by investigating absorption, excitation, and emission spectra of the glass samples. The absorption spectra were investigated in the UV-Vis-NIR region from 300 to 2000 nm. The excitation spectra observed under 574 nm emission wavelength showed the highest peak centered at 349 nm (6H15/2 → 6P7/2). The emission spectra, excited with 349 nm excitation wavelength showed two major peaks corresponding to 482 nm blue emission (4F9/2 → 6H15/2) and 574 nm yellow emission (4F9/2 → 6H13/2). The experimental lifetime were found to be 0.539 and 0.540 for oxide and oxyfluoride glass sample, respectively. The x,y color coordinates under 349 nm excitation wavelength were (0.38, 0.43) for both glass samples, that be plotted in white region of CIE 1931 chromaticity diagram. The CCT values obtained from the glass samples are 4204 K for oxide glass and 4228 K for oxyfluoride glass corresponding to the commercial cool white light (3100-4500 K). Judd-Ofelt theory had also been employed to obtain the J-O parameters (Ω2, Ω4 and Ω6), oscillator strength, radiative transition possibility, stimulated emission cross section and branching ratio. The Ω2 > Ω4 > Ω6 trend of J-O parameters of both glass samples may indicate the good quality of a glass host for using as optical device application. Temperature dependence of emission spectra was studied from 300 K to 10 K and found that the intensity of the emission peak was found to be increased with

  18. Multi-quasiparticle Excitations in 145Tb

    Institute of Scientific and Technical Information of China (English)

    ZhengYong; ZhouXiaohong; ZhangYuhu; T.Hayakawa; M.Oshima; T.Toh; T.Shizuma; J.Katakura; Y.Hatsukawa; M.Matsuda; H.Kusakari; M.Sugawara; K.Furuno; T.Komatsubara

    2003-01-01

    Study of in-beam γ-ray spectroscopy of 145Tb has been carried out by using 118Sn (32 S, 1p4n) 145Tb reaction. Excitation functions, X-γ-t and γ-γ-t coincidences and γ-ray anisotropies were measured. Here, t refers to the relative time difference between any two coincident γ-rays detected within ±200 ns. A level scheme of 14tTb, including 81 γ-transitions as shown in Fig.l, has been established up to 7.4 MeV in excitation energy and spinparity assignments for most of the observed levels have been done. The level structure shows characteristics of spherical nucleus. The observed states with excitation energies less than 2 MeV are interpreted by coupling an h11/2 proton to the 2+, 4+ and 3- core excitations in 144Gd. The excitation energies of these states fit wellin to the systematics of the neighboring odd-A N=80 isotones, and are well reproduced by the quasiparticle-cluster interaction calculations[1]. Semi-empirical shell model calculations[2] have been done for the higher-lying levels. The results clearly reveal the characteristic features of multiparticle configurations in a spherical nucleus. Specific configurations are proposed for the yrast and some non-yrast levels.

  19. Limits on violations of Lorentz symmetry and the Einstein equivalence principle using radio-frequency spectroscopy of atomic dysprosium.

    Science.gov (United States)

    Hohensee, M A; Leefer, N; Budker, D; Harabati, C; Dzuba, V A; Flambaum, V V

    2013-08-02

    We report a joint test of local Lorentz invariance and the Einstein equivalence principle for electrons, using long-term measurements of the transition frequency between two nearly degenerate states of atomic dysprosium. We present many-body calculations which demonstrate that the energy splitting of these states is particularly sensitive to violations of both special and general relativity. We limit Lorentz violation for electrons at the level of 10(-17), matching or improving the best laboratory and astrophysical limits by up to a factor of 10, and improve bounds on gravitational redshift anomalies for electrons by 2 orders of magnitude, to 10(-8). With some enhancements, our experiment may be sensitive to Lorentz violation at the level of 9 × 10(-20).

  20. Nonlinear optical properties of lutetium and dysprosium bisphthalocyanines at 1550 nm with femto- and nanosecond pulse excitation

    Science.gov (United States)

    Plekhanov, A. I.; Basova, T. V.; Parkhomenko, R. G.; Gürek, A. G.

    2017-02-01

    In this work, the nonlinear optical properties of unsubstituted lutetium (LuPc2) and dysprosium (DyPc2) bisphthalocyanines as well as octasubstituted Lu(PcR8)2 derivative with R=-S(C6H13) were studied at a wavelength of 1550 nm with 10 ns and 300 fs pulses. Based on Z-scan measurements the nonlinear absorption and refraction coefficient as well as the nature of nonlinear optical properties were analyzed for these materials. Open aperture Z-scan indicates strong two-photon absorption in all three bisphthalocyanines in nano- and femtosecond regimes. With good nonlinear optical coefficients, bisphthalocyanines of rare earth elements are expected to be promising materials for the creation of optical limiters.

  1. Spectroscopic data of the 1.8-, 2.9-, and 4.3- mu m transitions in dysprosium-doped gallium lanthanum sulfide glass

    Science.gov (United States)

    Schweizer, T.; Hewak, D. W.; Samson, B. N.; Payne, D. N.

    1996-10-01

    Infrared emission at 1.8, 2.9, and 4.3 mu m is measured in dysprosium-doped gallium lanthanum sulfide (Ga:La:S) glass excited at 815 nm. Emission cross sections were calculated by Judd-Ofelt analysis, the Fuchtbauer-Ladenburg equation, and the theory of McCumber. The sigma tau value for the 4.3- mu m transition is \\similar 4000 times larger in the Ga:La:S glass than in a dysprosium-doped LiYF4 crystal, which has lased on this transition. The large sigma tau value and the recently reported ability of Ga:La:S glass to be fabricated into fiber form show the potential for an efficient, low-threshold mid-infrared fiber laser. The fluorescence peak at 4.3 mu m coincides with the fundamental absorption of atmospheric carbon dioxide, making the glass a potential laser source for gas-sensing applications.

  2. Spectroscopic data of the 1.8-, 2.9-, and 4.3-microm transitions in dysprosium-doped gallium lanthanum sulfide glass.

    Science.gov (United States)

    Schweizer, T; Hewak, D W; Samson, B N; Payne, D N

    1996-10-01

    Infrared emission at 1.8, 2.9, and 4.3 microm is measured in dysprosium-doped gallium lanthanum sulfide (Ga:La:S) glass excited at 815 nm. Emission cross sections were calculated by Judd-Ofelt analysis, the Füchtbauer- Ladenburg equation, and the theory of McCumber. The sigmatau value for the 4.3-microm transition is ~4000 times larger in the Ga:La:S glass than in a dysprosium-doped LiYF(4) crystal, which has lased on this transition. The large sigmatau value and the recently reported ability of Ga:La:S glass to be fabricated into fiber form show the potential for an efficient, low-threshold mid-infrared fiber laser. The f luorescence peak at 4.3 microm coincides with the fundamental absorption of atmospheric carbon dioxide, making the glass a potential laser source for gas-sensing applications.

  3. On-line complexation/cloud point preconcentration for the sensitive determination of dysprosium in urine by flow injection inductively coupled plasma-optical emission spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Ortega, Claudia; Cerutti, Soledad; Silva, Maria F. [Departamento de Quimica Analitica, Facultad de Quimica, Bioquimica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, 5700, San Luis (Argentina); Olsina, Roberto A.; Martinez, Luis D. [Departamento de Quimica Analitica, Facultad de Quimica, Bioquimica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, 5700, San Luis (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Avda. Rivadavia 1917, CP C1033AAJJ, Buenos Aires (Argentina)

    2003-01-01

    An on-line dysprosium preconcentration and determination system based on the hyphenation of cloud point extraction (CPE) to flow injection analysis (FIA) associated with ICP-OES was studied. For the preconcentration of dysprosium, a Dy(III)-2-(5-bromo-2-pyridylazo)-5-diethylaminophenol complex was formed on-line at pH 9.22 in the presence of nonionic micelles of PONPE-7.5. The micellar system containing the complex was thermostated at 30 C in order to promote phase separation, and the surfactant-rich phase was retained in a microcolumn packed with cotton at pH 9.2. The surfactant-rich phase was eluted with 4 mol L{sup -1} nitric acid at a flow rate of 1.5 mL min{sup -1}, directly in the nebulizer of the plasma. An enhancement factor of 50 was obtained for the preconcentration of 50 mL of sample solution. The detection limit value for the preconcentration of 50 mL of aqueous solution of Dy was 0.03 {mu}g L{sup -1}. The precision for 10 replicate determinations at the 2.0 {mu}g L{sup -1}Dy level was 2.2% relative standard deviation (RSD), calculated from the peak heights obtained. The calibration graph using the preconcentration system for dysprosium was linear with a correlation coefficient of 0.9994 at levels near the detection limits up to at least 100 {mu}g L {sup -1}. The method was successfully applied to the determination of dysprosium in urine. (orig.)

  4. 32 CFR 842.145 - Delegation of authority.

    Science.gov (United States)

    2010-07-01

    ... General has delegated authority to make an advance payment of $100,000 or less. (c) The following individuals have delegated authority to make an advance payment of $25,000 or less: (1) The Deputy Judge... 32 National Defense 6 2010-07-01 2010-07-01 false Delegation of authority. 842.145 Section 842.145...

  5. 40 CFR 145.12 - Requirements for compliance evaluation programs.

    Science.gov (United States)

    2010-07-01

    ... for compliance evaluation programs. (a) State programs shall have procedures for receipt, evaluation... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Requirements for compliance evaluation programs. 145.12 Section 145.12 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED)...

  6. 19 CFR 145.24 - Amendment of entry.

    Science.gov (United States)

    2010-04-01

    ... director is satisfied that the objection is valid and timely, he shall amend the mail entry. If the duty... 19 Customs Duties 2 2010-04-01 2010-04-01 false Amendment of entry. 145.24 Section 145.24 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE...

  7. 14 CFR 145.103 - Housing and facilities requirements.

    Science.gov (United States)

    2010-01-01

    ..., Materials, and Data § 145.103 Housing and facilities requirements. (a) Each certificated repair station must provide— (1) Housing for the facilities, equipment, materials, and personnel consistent with its ratings... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Housing and facilities requirements. 145...

  8. 21 CFR 163.145 - Mixed dairy product chocolates.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Mixed dairy product chocolates. 163.145 Section... § 163.145 Mixed dairy product chocolates. (a) Description. Mixed dairy product chocolates are the foods...; or (iv) Malted milk; and (2) The finished mixed dairy product chocolates shall contain not less...

  9. 9 CFR 319.145 - Italian sausage products.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Italian sausage products. 319.145... Sausage § 319.145 Italian sausage products. (a) Italian sausage products are cured or uncured sausages... optional ingredients listed in paragraph (b) of this section. (1) “Italian Sausage” shall be prepared with...

  10. 9 CFR 145.54 - Terminology and classification; States.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Terminology and classification; States. 145.54 Section 145.54 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... Terminology and classification; States. (a) U.S. Pullorum-Typhoid Clean State. (1) A State will be declared...

  11. 21 CFR 146.145 - Orange juice from concentrate.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Orange juice from concentrate. 146.145 Section 146... Juices and Beverages § 146.145 Orange juice from concentrate. (a) Orange juice from concentrate is the food prepared by mixing water with frozen concentrated orange juice as defined in § 146.146 or...

  12. 21 CFR 145.126 - Artificially sweetened canned cherries.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Artificially sweetened canned cherries. 145.126... § 145.126 Artificially sweetened canned cherries. (a) Artificially sweetened canned cherries is the food which conforms to the definition and standard of identity prescribed for canned cherries by §...

  13. 24 CFR 100.145 - Loss of privilege.

    Science.gov (United States)

    2010-04-01

    ... DISCRIMINATORY CONDUCT UNDER THE FAIR HOUSING ACT Discrimination in Residential Real Estate-Related Transactions... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Loss of privilege. 100.145 Section 100.145 Housing and Urban Development Regulations Relating to Housing and Urban Development OFFICE...

  14. 19 CFR 145.53 - Firearms and munitions of war.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Firearms and munitions of war. 145.53 Section 145.53 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF... munitions of war. Importations of firearms, munitions of war, and related articles are subject to the import...

  15. 21 CFR 133.145 - Granular cheese for manufacturing.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Granular cheese for manufacturing. 133.145 Section... Standardized Cheese and Related Products § 133.145 Granular cheese for manufacturing. Granular cheese for manufacturing conforms to the definition and standard of identity prescribed for granular cheese by § 133.144...

  16. 19 CFR 145.43 - Unaccompanied tourist shipments

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Unaccompanied tourist shipments 145.43 Section 145.43 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF... tourist shipments Unaccompanied tourist shipments for which entry is claimed under subheading...

  17. Synthesis and Crystal Structure of Tri-(2-mercaptopyridine N-oxide)bis(dimethyl sulfoxide) Dysprosium(Ⅲ)

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A range of rare earth metal complexes of 2-mercaptopyridine N-oxide (Hmpo) have been synthesized, and studied by elemental analysis and IR spectroscopic technique. Crystal structure of Dy(mpo)3(DMSO)2 (DMSO = dimethyl sulfoxide) has been determined. The complex crystallizes in the triclinic system, space group Pī with lattice parameters: a = 9.602(3), b = 9.803(3), c = 15.498(5)A, α= 89.51(1), β= 85.73(1), γ= 62.99(1)°, Dc = 1.787 g/cm3, C19H24N3O5S5Dy, Mr = 697.21, Z = 2, F(000) = 690, μ = 3.321mm-1, the final R = 0.0237 and wR = 0.0587 for 4116 reflections with I>σ2(I). The coordination number of dysprosium Ⅲ is eight, and its coordination geometry is a somewhat distorted square antiprism with O(3), O(4), O(5), S(3) and O(1), O(2), S(1), S(2) at the tetragonal bases (dihedral angle between their mean planes is 2.9(1)0). Around the Dy atom, three five-membered ring planes (Dy, O, N, C, S) make the dihedral angles of 74.42, 11.31 and 83.72, respectively.

  18. Photo-, cathodo- and thermoluminescent properties of dysprosium-doped HfO2 films deposited by ultrasonic spray pyrolysis.

    Science.gov (United States)

    Manríquez, R Reynoso; Góngora, J A I Díaz; Guzmán-Mendoza, J; Montalvo, T Rivera; Olguín, J C Guzmán; Ramírez, P V Cerón; García-Hipólito, M; Falcony, C

    2014-09-01

    In this work, the photoluminescent (PL), cathodoluminescent (CL) and thermoluminescent (TL) properties of hafnium oxide films doped with trivalent dysprosium ions are reported. The films were deposited on glass substrates at temperatures ranging from 300 to 600°C, using chlorides as precursor reagents. The surface morphology of films showed a veins shaped microstructure at low deposition temperatures, while at higher temperatures the formation of spherical particles was observed on the surface. X-ray diffraction showed the presence of HfO2 monoclinic phase in the films deposited at temperatures greater than 400°C. The PL and CL spectra of the doped films showed the highest emission band centered at 575nm corresponding to the transitions (4)F9/2→(6)H13/2, which is a characteristic transition of Dy(3+) ion. The greatest emission intensities were observed in samples doped with 1 atomic percent (at%) of DyCl3 in the precursor solution. Regarding the TL behavior, the glow curve of HfO2:Dy(+3) films exhibited spectrum with one broad band centered at about 150°C. The highest intensity TL response was observed on the films deposited at 500°C. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Workplace testing of the new single sphere neutron spectrometer based on Dysprosium activation foils (Dy-SSS)

    Science.gov (United States)

    Bedogni, R.; Gómez-Ros, J. M.; Esposito, A.; Gentile, A.; Chiti, M.; Palacios-Pérez, L.; Angelone, M.; Tana, L.

    2012-08-01

    A photon insensitive passive neutron spectrometer consisting of a single moderating polyethylene sphere with Dysprosium activation foils arranged along three perpendicular axes was designed by CIEMAT and INFN. The device is called Dy-SSS (Dy foil-based Single Sphere Spectrometer). It shows nearly isotropic response in terms of neutron fluence up to 20 MeV. The first prototype, previously calibrated with 14 MeV neutrons, has been recently tested in workplaces having different energy and directional distributions. These are a 2.5 MeV nearly mono-chromatic and mono-directional beam available at the ENEA Frascati Neutron Generator (FNG) and the photo-neutron field produced in a 15 MV Varian CLINAC DHX medical accelerator, located in the Ospedale S. Chiara (Pisa). Both neutron spectra are known through measurements with a Bonner Sphere Spectrometer. In both cases the experimental response of the Dy-SSS agrees with the reference data. Moreover, it is demonstrated that the spectrometric capability of the new device are independent from the directional distribution of the neutron field. This opens the way to a new generation of moderation-based neutron instruments, presenting all advantages of the Bonner sphere spectrometer without the disadvantage of the repeated exposures. This concept is being developed within the NESCOFI@BTF project of INFN (Commissione Scientifica Nazionale 5).

  20. Ferroelectric properties of dysprosium-doped Bi4Ti3O12 thin films crystallized in various atmospheres

    Institute of Scientific and Technical Information of China (English)

    CHENG Chuan-pin; TANG Ming-hua; YE Zhi; ZHOU Yic-hun; ZHENG Xue-jun; ZHONG Xiang-li; HU Zeng-shun

    2006-01-01

    Dysprosium-doped Bi4Ti3O12 (Bi3.4Dy0.6Ti3O12,BDT) ferroelectric thin films were deposited on Pt(111)/Ti/SiO2/Si(111) substrates by chemical solution deposition (CSD) and crystallized in nitrogen,air and oxygen atmospheres,respectively. X-ray diffraction (XRD) and scanning electron microscopy (SEM) were used to identify the crystal structure,the surface and cross-section morphology of the deposited ferroelectric films. The results show that the crystallization atmosphere has significant effect on determining the crystallization and ferroelectric properties of the BDT films. The film crystallized in nitrogen at a relatively low temperature of 650 ℃,exhibits excellent crystallinity and ferroelectricity with a remanent polarization of 2Pr = 24.9 μC/cm2 and a coercive field of 144.5 kV/cm. While the films annealed in air and oxygen at 650 ℃ do not show good crystallinity and ferroelectricity until they are annealed at 700 ℃. The structure evolution and ferroelectric properties of BDT thin films annealed under different temperatures (600-750 ℃) were also investigated. The crystallinity of the BDT films is improved and the average grain size increases when the annealing temperature increases from 600 ℃ to 750 ℃ at an interval of 50 ℃. However,the polarization of the films is not monotonous function of the annealing temperature.

  1. 22 CFR 145.28 - Period of availability of funds.

    Science.gov (United States)

    2010-04-01

    ... HIGHER EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management § 145.28 Period of availability of funds. Where a funding period is specified,...

  2. 27 CFR 25.145 - Tanks, vehicles, and vessels.

    Science.gov (United States)

    2010-04-01

    ... BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Marks, Brands, and Labels § 25.145 Tanks, vehicles, and... mark each tank, tank car, tank truck, tank ship, barge, or deep tank of a vessel in accordance...

  3. 22 CFR 145.23 - Cost sharing or matching.

    Science.gov (United States)

    2010-04-01

    ... Management § 145.23 Cost sharing or matching. (a) All contributions, including cash and third party in-kind... expendable equipment, office supplies, laboratory supplies or workshop and classroom supplies. Value assessed...

  4. Sensitive search for the temporal variation of the fine structure constant using radio-frequency E1 transitions in atomic dysprosium

    CERN Document Server

    Nguyen, A T; Lamoreaux, S K; Torgerson, J R

    2003-01-01

    It has been proposed that the radio-frequency electric-dipole (E1) transition between two nearly degenerate opposite-parity states in atomic dysprosium should be highly sensitive to possible temporal variation of the fine structure constant ($\\alpha$) [V. A. Dzuba, V. V. Flambaum, and J. K. Webb, Phys. Rev. A {\\bf 59}, 230 (1999)]. We discuss here an experimental realization of the proposed search, which involves monitoring the E1 transition frequency over a period of time using direct frequency counting techniques. We estimate that a statistical sensitivity of $|\\adota| \\sim 10^{-18}$/yr may be achieved and discuss possible systematic effects in such a measurement.

  5. Dysprosium doping induced shape and magnetic anisotropy of Fe3-xDyxO4 (x=0.01-0.1) nanoparticles

    Science.gov (United States)

    Jain, Richa; Luthra, Vandna; Gokhale, Shubha

    2016-09-01

    The effect of dysprosium doping on evolution of structural and magnetic properties of magnetite (Fe3O4) nanoparticles is reported. A standard route of co-precipitation was used for the synthesis of undoped and doped magnetite nanoparticles Fe3-xDyxO4 (x=0.0-0.1). Transmission electron microscopy (TEM) shows formation of round shaped particles with diameter in the range of 8-14 nm for undoped sample. On doping beyond x=0.01, the formation of rod like structures is initiated along with the round shaped particles. The number of rods is found to increase with increasing doping concentration. Magnetic characterization using Vibrating Sample Magnetometer (VSM) revealed doping dependent magnetic properties which can be correlated with the crystallite size as determined from X-ray diffraction (XRD). Enhancement in the saturation magnetization in the initial stages of doping can be explained on the basis of incorporation of Dy3+ ions in the inverse spinel structure at the octahedral site in place of Fe3+ ions. Subsequent decrease in saturation magnetization observed beyond x=0.03 could be attributed to precipitation of excess Dy in form of dysprosium ferrite phase.

  6. 14 CFR 135.145 - Aircraft proving and validation tests.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Aircraft proving and validation tests. 135... Aircraft and Equipment § 135.145 Aircraft proving and validation tests. (a) No certificate holder may... safely and in compliance with applicable regulatory standards. Validation tests are required for...

  7. 40 CFR 35.145 - Maximum federal share.

    Science.gov (United States)

    2010-07-01

    ... STATE AND LOCAL ASSISTANCE Environmental Program Grants Air Pollution Control (section 105) § 35.145 Maximum federal share. (a) The Regional Administrator may provide air pollution control agencies, as... programs for the prevention and control of air pollution or implementing national primary and...

  8. 9 CFR 145.34 - Terminology and classification; States.

    Science.gov (United States)

    2010-01-01

    ... Special Provisions for Multiplier Meat-Type Chicken Breeding Flocks and Products § 145.34 Terminology and... have existed in meat-type chicken breeding flocks in production within the State during the preceding 12 months; (ii) All meat-type chicken breeding flocks in production are classified as U.S. M...

  9. 9 CFR 145.24 - Terminology and classification; States.

    Science.gov (United States)

    2010-01-01

    ..., DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Multiplier Egg-Type Chicken Breeding Flocks and Products § 145.24 Terminology and... found within the preceding 24 months in waterfowl, exhibition poultry, and game bird breeding flocks...

  10. 14 CFR 145.209 - Repair station manual contents.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Repair station manual contents. 145.209... manual contents. A certificated repair station's manual must include the following: (a) An organizational... for revising the repair station's manual and notifying its certificate holding district office...

  11. 14 CFR 145.109 - Equipment, materials, and data requirements.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Equipment, materials, and data requirements... TRANSPORTATION (CONTINUED) SCHOOLS AND OTHER CERTIFICATED AGENCIES REPAIR STATIONS Housing, Facilities, Equipment, Materials, and Data § 145.109 Equipment, materials, and data requirements. (a) Except as otherwise...

  12. 24 CFR 891.145 - Owner deposit (Minimum Capital Investment).

    Science.gov (United States)

    2010-04-01

    ... General Program Requirements § 891.145 Owner deposit (Minimum Capital Investment). As a Minimum Capital... Investment shall be one-half of one percent (0.5%) of the HUD-approved capital advance, not to exceed $25,000. ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Owner deposit (Minimum...

  13. 22 CFR 145.26 - Non-Federal audits.

    Science.gov (United States)

    2010-04-01

    ... Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS GRANTS AND AGREEMENTS WITH INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management § 145.26 Non-Federal audits. (a) Recipients and subrecipients that are institutions of...

  14. 19 CFR 145.12 - Entry of merchandise.

    Science.gov (United States)

    2010-04-01

    ... stamp all mail articles in the shipment to show that they have received Customs treatment and shall... Articles, Customs Form 255, affixed to the shipment shall be removed by the Customs officer and retained... § 145.12, see the List of CFR Sections Affected, which appears in the Finding Aids section of...

  15. Regulation of inositol 1,4,5-trisphosphate receptor expression

    NARCIS (Netherlands)

    Deelman, Leo Edwin

    1999-01-01

    This thesis describes the regulation of the inositol 1,4,5-triphosphate receptor (InsP3R). The mechanisms of InsP3R regulation were investigated after cloning of the promoters of the human and rat InsP3R-1 gene and visualization of subcellular distribution of InsP3R-3 by immunoelectron microscopy (c

  16. 20 CFR 402.145 - Responding to your request.

    Science.gov (United States)

    2010-04-01

    ... THE PUBLIC § 402.145 Responding to your request. (a) Retrieving records. We are required to furnish... perform research for you. We may decide to conserve Government resources and at the same time supply the... that is exempt from disclosure. For example, in an opinion or order, statement of policy, or...

  17. 9 CFR 145.8 - Terminology and classification; general.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Terminology and classification... General Provisions § 145.8 Terminology and classification; general. (a) The official classification terms... identity under Plan terminology when they are purchased for resale by or consigned to nonparticipants....

  18. 22 CFR 145.15 - Metric system of measurement.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Metric system of measurement. 145.15 Section... system of measurement. The Metric Conversion Act, as amended by the Omnibus Trade and Competitiveness Act (15 U.S.C. 205) declares that the metric system is the preferred measurement system for U.S. trade...

  19. 21 CFR 145.171 - Artificially sweetened canned peaches.

    Science.gov (United States)

    2010-04-01

    ... thickened with pectin and may contain any mixture of any edible organic salt or salts and any edible organic... used, as prescribed for canned peaches by § 145.170(a). If the packing medium is thickened with pectin, the label shall bear the statement “thickened with pectin”. When any organic salt or acid or...

  20. 21 CFR 145.176 - Artificially sweetened canned pears.

    Science.gov (United States)

    2010-04-01

    ... thickened with pectin and may contain any mixture of any edible organic salt or salts and any edible organic..., as prescribed for canned pears by § 145.175(a). If the packing medium is thickened with pectin, the label shall bear the statement “thickened with pectin”. When any organic salt or acid or any mixture...

  1. 21 CFR 145.131 - Artificially sweetened canned figs.

    Science.gov (United States)

    2010-04-01

    ... with pectin and may contain any mixture of any edible organic salt or salts and any edible organic acid... prescribed for canned figs by § 145.130. If the packing medium is thickened with pectin, the label shall bear the statement “thickened with pectin”. When any organic salt or acid or any mixture of two or more...

  2. 22 CFR 145.11 - Pre-award policies.

    Science.gov (United States)

    2010-04-01

    ..., “substantial involvement is expected between the executive agency and the State, local government, or other... EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Pre-Award Requirements § 145.11 Pre-award policies... Government. The Department may not award grants or cooperative agreements unless specific statutory authority...

  3. MicroRNA-145 regulates human corneal epithelial differentiation.

    Directory of Open Access Journals (Sweden)

    Sharon Ka-Wai Lee

    Full Text Available BACKGROUND: Epigenetic factors, such as microRNAs, are important regulators in the self-renewal and differentiation of stem cells and progenies. Here we investigated the microRNAs expressed in human limbal-peripheral corneal (LPC epithelia containing corneal epithelial progenitor cells (CEPCs and early transit amplifying cells, and their role in corneal epithelium. METHODOLOGY/PRINCIPAL FINDINGS: Human LPC epithelia was extracted for small RNAs or dissociated for CEPC culture. By Agilent Human microRNA Microarray V2 platform and GeneSpring GX11.0 analysis, we found differential expression of 18 microRNAs against central corneal (CC epithelia, which were devoid of CEPCs. Among them, miR-184 was up-regulated in CC epithelia, similar to reported finding. Cluster miR-143/145 was expressed strongly in LPC but weakly in CC epithelia (P = 0.0004, Mann-Whitney U-test. This was validated by quantitative polymerase chain reaction (qPCR. Locked nucleic acid-based in situ hybridization on corneal rim cryosections showed miR-143/145 presence localized to the parabasal cells of limbal epithelium but negligible in basal and superficial epithelia. With holoclone forming ability, CEPCs transfected with lentiviral plasmid containing mature miR-145 sequence gave rise to defective epithelium in organotypic culture and had increased cytokeratin-3/12 and connexin-43 expressions and decreased ABCG2 and p63 compared with cells transfected with scrambled sequences. Global gene expression was analyzed using Agilent Whole Human Genome Oligo Microarray and GeneSpring GX11.0. With a 5-fold difference compared to cells with scrambled sequences, miR-145 up-regulated 324 genes (containing genes for immune response and down-regulated 277 genes (containing genes for epithelial development and stem cell maintenance. As validated by qPCR and luciferase reporter assay, our results showed miR-145 suppressed integrin β8 (ITGB8 expression in both human corneal epithelial cells

  4. Compaction of insulated 145 kV electric power substations; Compactacao de subestacoes abrigadas de 145 kV

    Energy Technology Data Exchange (ETDEWEB)

    Sueta, Helio Eiji; Castro Neto, Gervasio L. [Sao Paulo Univ., SP (Brazil). Inst. de Eletrotecnica e Energia; Porto, Walmar Freitas [Eletricidade de Sao Paulo, Sao Paulo, SP (Brazil)

    1995-12-31

    Considering the increasing reduction in the areas of insulated 145 kV electric power substations, the most commonly adopted solution has been the vertical organization. This work proposes the reduction of the total area through the reduction of the area designed to high voltage installations, using smaller phase-to-phase distances than the traditionally used ones 5 refs., 4 figs.

  5. The -145 km/S Absorption System of Eta Carinae

    Science.gov (United States)

    Vieira, G.; Gull, T. R.; Danks, A.; Johansson, S.

    2002-01-01

    With the STIS E230H mode (R-118,000) , we have identified about twenty absorption components in line of sight from Eta Carinae. Two components, one at -513 km/s and another at -145 W s , are quite different in character from the others, mostly at intermediate velocities. The -145 km/s component is significantly wider in fwhm, is seen in many more species, and the lower level can be above 20,000/cm, well above the 2000/cm noted in the -513 km/s component. In the spectral region from 2400 to 3160A, approximately 500 absorption lines have been identified. In this poster, we will present line identifications and atomic parameters of the measured lines, hopefully providing insight as to what levels are being excited and by what processes.

  6. Single-molecule magnet behavior in an octanuclear dysprosium(iii) aggregate inherited from helical triangular Dy3 SMM-building blocks.

    Science.gov (United States)

    Zhang, Li; Zhang, Peng; Zhao, Lang; Wu, Jianfeng; Guo, Mei; Tang, Jinkui

    2016-06-28

    An unprecedented octanuclear dysprosium(iii) cluster with the formula [Dy8L6(μ3-OH)4(μ2-CH3O)2(CH3OH)6(H2O)2]·6H2O·10CH3OH·2CH3CN () based on a nonlinearly tritopic aroylhydrazone ligand H3L has been isolated, realizing the successful linking of pairwise interesting triangular Dy3 SMMs. It is noteworthy that two enantiomers (Λ and Δ configurations) individually behaving as a coordination-induced chirality presented in the Dy3 helicate are connected in the meso Dy8 cluster. Remarkably, alternating-current magnetic susceptibility measurements revealed that the Dy8 cluster shows typical SMM behavior inherited from its Dy3 helical precursor. It is one of the rare polynuclear Lnn SMMs (n > 7) under zero dc field.

  7. Another challenge to paramagnetic relaxation theory: a study of paramagnetic proton NMR relaxation in closely related series of pyridine-derivatised dysprosium complexes.

    Science.gov (United States)

    Rogers, Nicola J; Finney, Katie-Louise N A; Senanayake, P Kanthi; Parker, David

    2016-02-14

    Measurements of the relaxation rate behaviour of two series of dysprosium complexes have been performed in solution, over the field range 1.0 to 16.5 Tesla. The field dependence has been modelled using Bloch-Redfield-Wangsness theory, allowing estimates of the electronic relaxation time, T1e, and the size of the magnetic susceptibility, μeff, to be made. Changes in relaxation rate of the order of 50% at higher fields were measured, following variation of the para-substituent in the single pyridine donor. The magnetic susceptibilities deviated unexpectedly from the free-ion values for certain derivatives in each series examined, in a manner that was independent of the electron-releasing/withdrawing ability of the pyridine substituent, suggesting that the polarisability of just one pyridine donor in octadenate ligands can play a significant role in defining the magnetic susceptibility anisotropy.

  8. 21 CFR 145.135 - Canned fruit cocktail.

    Science.gov (United States)

    2010-04-01

    ... § 145.3 are: (a) Water. (b) Fruit juice(s) and water. (c) Fruit juice(s). Such packing media may be used... juice(s) and water”; or “slightly sweetened fruit juice(s)”, as the case may be. (b) When the density of... sirup”; “lightly sweetened fruit juice(s) and water”; or “lightly sweetened fruit juice(s)”, as the......

  9. Phosphorylation of the rat Ins(1,4,5)P₃ receptor at T930 within the coupling domain decreases its affinity to Ins(1,4,5)P₃.

    Science.gov (United States)

    Haun, Shirley; Sun, Lu; Hubrack, Satanay; Yule, David; Machaca, Khaled

    2012-01-01

    The Ins(1,4,5)P 3 receptor acts as a central hub for Ca ( 2+) signaling by integrating multiple signaling modalities into Ca ( 2+) release from intracellular stores downstream of G-protein and tyrosine kinase-coupled receptor stimulation. As such, the Ins(1,4,5)P 3 receptor plays fundamental roles in cellular physiology. The regulation of the Ins(1,4,5)P 3 receptor is complex and involves protein-protein interactions, post-translational modifications, allosteric modulation, and regulation of its sub-cellular distribution. Phosphorylation has been implicated in the sensitization of Ins(1,4,5)P 3-dependent Ca ( 2+) release observed during oocyte maturation. Here we investigate the role of phosphorylation at T-930, a residue phosphorylated specifically during meiosis. We show that a phosphomimetic mutation at T-930 of the rat Ins(1,4,5)P 3 receptor results in decreased Ins(1,4,5)P 3-dependent Ca ( 2+) release and lowers the Ins(1,4,5)P 3 binding affinity of the receptor. These data, coupled to the sensitization of Ins(1,4,5)P 3-dependent Ca ( 2+) release during meiosis, argue that phosphorylation within the coupling domain of the Ins(1,4,5)P 3 receptor acts in a combinatorial fashion to regulate Ins(1,4,5)P 3 receptor function.

  10. 19 CFR 145.39 - Articles for diplomatic officers, representatives of international organizations, and foreign...

    Science.gov (United States)

    2010-04-01

    ... of international organizations, and foreign military personnel. 145.39 Section 145.39 Customs Duties..., representatives of international organizations, and foreign military personnel. Free entry of articles in mail articles addressed to diplomatic officers, representatives of certain international organizations,...

  11. 37 CFR 1.145 - Subsequent presentation of claims for different invention.

    Science.gov (United States)

    2010-07-01

    ... claims for different invention. 1.145 Section 1.145 Patents, Trademarks, and Copyrights UNITED STATES... Processing Provisions Joinder of Inventions in One Application; Restriction § 1.145 Subsequent presentation of claims for different invention. If, after an Office action on an application, the applicant...

  12. 17 CFR 230.145 - Reclassification of securities, mergers, consolidations and acquisitions of assets.

    Science.gov (United States)

    2010-04-01

    ..., mergers, consolidations and acquisitions of assets. 230.145 Section 230.145 Commodity and Securities... § 230.145 Reclassification of securities, mergers, consolidations and acquisitions of assets.... A statutory merger or consolidation or similar plan or acquisition in which securities of...

  13. 45 CFR 146.145 - Special rules relating to group health plans.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Special rules relating to group health plans. 146.145 Section 146.145 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO....145 Special rules relating to group health plans. (a) Group health plan—(1) Definition. A group...

  14. 9 CFR 145.10 - Terminology and classification; flocks, products, and States.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Terminology and classification; flocks, products, and States. 145.10 Section 145.10 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION... POULTRY General Provisions § 145.10 Terminology and classification; flocks, products, and...

  15. 9 CFR 145.43 - Terminology and classification; flocks and products.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Terminology and classification; flocks and products. 145.43 Section 145.43 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION... POULTRY Special Provisions for Turkey Breeding Flocks and Products § 145.43 Terminology and...

  16. 9 CFR 145.9 - Terminology and classification; hatcheries and dealers.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Terminology and classification; hatcheries and dealers. 145.9 Section 145.9 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION... POULTRY General Provisions § 145.9 Terminology and classification; hatcheries and dealers....

  17. 21 CFR 173.145 - Alpha-Galactosidase derived from Mortierella vinaceae var. raffinoseutilizer.

    Science.gov (United States)

    2010-04-01

    ... FOOD ADDITIVES PERMITTED IN FOOD FOR HUMAN CONSUMPTION Enzyme Preparations and Microorganisms § 173.145... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Alpha-Galactosidase derived from Mortierella vinaceae var. raffinoseutilizer. 173.145 Section 173.145 Food and Drugs FOOD AND DRUG...

  18. 27 CFR 70.145 - Purchasers, holders of security interests, mechanic's lienors, and judgment lien creditors.

    Science.gov (United States)

    2010-04-01

    ... security interests, mechanic's lienors, and judgment lien creditors. 70.145 Section 70.145 Alcohol, Tobacco... Lien for Taxes § 70.145 Purchasers, holders of security interests, mechanic's lienors, and judgment... in § 70.143(a) of this part), mechanic's lienor (as defined in § 70.143(b) of this part), or...

  19. Pharmacological Characterization of Inositol 1,4,5-tris Phosphate Receptors in Human Platelet Membranes

    Directory of Open Access Journals (Sweden)

    Yogesh Dwivedi

    2009-01-01

    Full Text Available The phosphatidylinositol (PI hydrolysis signaling system has been shown to be altered in platelets of depressed and schizophrenic subjects. Inositol (1,4,5 trisphosphate (Ins(1,4,5P3, an integral component of the PI signaling system, mobilizes Ca2+ by activating Ins(1,4,5P3 receptors. To eventually investigate the role of Ins(1,4,5P3 receptors in depression and other mental disorders, we characterized [H3]Ins(1,4,5P3 binding sites in crude platelet membranes prepared from small amounts of blood obtained from healthy human control subjects. We found a single, saturable binding site for [H3]Ins(1,4,5P3 to crude platelet membranes, which is time dependent and modulated by pH, inositol phosphates, and heparin. Since cyclic adenosine monophosphate (cAMP and Ca2+ have been shown to be important modulators in Ins(1,4,5P3 receptors, in the present study we also determined the effects of various concentrations of CaCI2 and forskolin on Ins(1,4,5P3 binding to platelet membranes. CaCI2 modulated [3H]Ins(1,4,5P3 binding sites in a biphasic manner: at lower concentrations it inhibited [3H]Ins(1,4,5P3 binding, whereas at higher concentrations, it stimulated [3H]Ins(1,4,5P3 binding. On the other hand, forskolin inhibited [3H]Ins(1,4,5P3 binding. Our results thus suggest that the pharmacological characteristics of [3H]Ins(1,4,5P3 binding to crude platelet membranes are similar to that of Ins(1,4,5P3 receptors; and that both Ca2+ and cAMP modulate [3H]Ins(1,4,5P3 binding in crude platelet membranes.

  20. Regulation of UHRF1 by dual-strand tumor-suppressor microRNA-145 (miR-145-5p and miR-145-3p): inhibition of bladder cancer cell aggressiveness

    Science.gov (United States)

    Matsushita, Ryosuke; Yoshino, Hirofumi; Enokida, Hideki; Goto, Yusuke; Miyamoto, Kazutaka; Yonemori, Masaya; Inoguchi, Satoru; Nakagawa, Masayuki; Seki, Naohiko

    2016-01-01

    In microRNA (miRNA) biogenesis, the guide-strand of miRNA integrates into the RNA induced silencing complex (RISC), whereas the passenger-strand is inactivated through degradation. Analysis of our miRNA expression signature of bladder cancer (BC) by deep-sequencing revealed that microRNA (miR)-145-5p (guide-strand) and miR-145-3p (passenger-strand) were significantly downregulated in BC tissues. It is well known that miR-145-5p functions as a tumor suppressor in several types of cancer. However, the impact of miR-145-3p on cancer cells is still ambiguous. The aim of the present study was to investigate the functional significance of miR-145-3p and BC oncogenic pathways and targets regulated by miR-145-5p/miR-145-3p. Ectopic expression of either miR-145-5p or miR-145-3p in BC cells significantly suppressed cancer cell growth, migration and invasion and it also induced apoptosis. The gene encoding ubiquitin-like with PHD and ring finger domains 1 (UHRF1) was a direct target of these miRNAs. Silencing of UHRF1 induced apoptosis and inhibited cancer cell proliferation, migration, and invasion in BC cells. In addition, overexpressed UHRF1 was confirmed in BC clinical specimens, and the high UHRF1 expression group showed a significantly poorer cause specific survival rate in comparison with the low expression group. Taken together, our present data demonstrated that both strands of miR-145 (miR-145-5p: guide-strand and miR-145-3p: passenger-strand) play pivotal roles in BC cells by regulating UHRF1. The identification of the molecular target of a tumor suppressive miRNAs provides novel insights into the potential mechanisms of BC oncogenesis and suggests novel therapeutic strategies. PMID:27072587

  1. Tuning the composition and magnetostructure of dysprosium iron garnets by Co-substitution: An XRD, FT-IR, XPS and VSM study

    Energy Technology Data Exchange (ETDEWEB)

    Tholkappiyan, R.; Vishista, K., E-mail: raovishista@gmail.com

    2015-10-01

    Graphical abstract: - Highlights: • Garnet type Dy{sub 3}Fe{sub 5−x}Co{sub x}O{sub 12} (x = 0–0.06) nanoparticles were synthesized by glycine assisted combustion method. • To investigate and confirm the phases in the synthesized ferrite nanoparticles by FT-IR and XRD analysis. • To investigate the compositional and oxidation state of the samples by X-ray photoelectron spectroscopy. • The detailed core level spectra of Dy 4d, Fe 2p, Co 2p and O 1s were analyzed using XPS. • The magnetic property was studied by VSM technique. - Abstract: We report the Co-substituting on the synthesis and properties of garnet type dysprosium ferrite nanoparticles by basic composition Dy{sub 3}Fe{sub 5−x}Co{sub x}O{sub 12} (x = 0–0.06) synthesized through glycine assisted combustion method. A possible formation mechanism of synthesized Dy{sub 3}Fe{sub 5−x}Co{sub x}O{sub 12} samples by controlling the synthesis process has been proposed. XRD, FT-IR, XPS and VSM studies were used to investigate the compositional and magnetostructural properties of the prepared nanoparticles. XRD results confirm that all the samples are single-phase cubic garnet structure with mean crystallite size of 97–105 nm obtained from Scherrer method and 95–102 nm from W–H method. FT-IR analysis shows the presence of three expected bands in the frequency limit of 450–600 cm{sup −1} attributed to metal–O stretching vibration in tetrahedral site of garnet structure. A typical survey spectrum from XPS results confirmed the presence of Dy, Fe, Co and O elements in the samples. This study also to characterize the different oxidation states of the samples by fitting the parameters of high resolution Dy 4d, Fe 2p, Co 2p and O 1s XPS spectra. The XPS data of Dy 4d spectrum show that Dy{sup 3+} ion occupy in dodecahedral (D) site. The XPS analysis of Fe 2p and Co 2p data suggests that (Fe{sup 3+} and Fe{sup 2+}), (Co{sup 3+} and Co{sup 2+}) are distributed in tetrahedral and octahedral sites

  2. MiR-145 functions as a tumor suppressor targeting NUAK1 in human intrahepatic cholangiocarcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Xiong, Xinkui; Sun, Daoyi; Chai, Hao; Shan, Wengang [Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province (China); Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Nanjing, Jiangsu Province (China); Yu, Yue [Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Nanjing, Jiangsu Province (China); Pu, Liyong [Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province (China); Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Nanjing, Jiangsu Province (China); Cheng, Feng, E-mail: docchengfeng@njmu.edu.cn [Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province (China); Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Nanjing, Jiangsu Province (China)

    2015-09-18

    The dysregulation of micro (mi)RNAs is associated with cancer development. The miRNA miR-145 is downregulated in intrahepatic cholangiocarcinoma (ICC); however, its precise role in tumor progression has not yet been elucidated. Novel (nua) kinase family (NUAK)1 functions as an oncogene in various cancers and is a putative target of miR-145 regulation. In this study, we investigated the regulation of NUAK1 by miR-145 in ICC. We found that miR-145 level was significantly decreased in ICC tissue and cell lines, which corresponded with an increase in NUAK1 expression. NUAK1 was found to be a direct target of miR-145 regulation. The overexpression of miR-145 in ICC cell lines inhibited proliferation, growth, and invasion by suppressing NUAK1 expression, which was associated with a decrease in Akt signaling and matrix metalloproteinase protein expression. Similar results were observed by inhibiting NUAK1 expression. These results demonstrate that miR-145 can prevent ICC progression by targeting NUAK1 and its downstream effectors, and can therefore be useful for clinical diagnosis and targeted therapy of ICC. - Highlights: • MiR-145 suppresses ICC proliferation and invasion abilities. • We demonstrated that miR-145 directly targets NUAK1 in ICC. • MiR-145 expression in ICC was associated with Akt signaling and MMPs expression.

  3. Experiments on a 14.5 GHz ECR source

    CERN Document Server

    Hill, C E

    1998-01-01

    The 14.5 GHz ECR4 source supplied to CERN in the framework of the Heavy Ion Facility collaboration provided Pb27+ operational beams to a new heavy ion linac in 1994. This source, which operates in the pulsed "afterglow" mode, has surpassed its design specification of 80 emA and now provides currents >120 emA on an operational basis for this charge state. Early tests showed the existence of extremely stable modes of operation which are fully exploited, and in 1996 the source was operational on a 24 hour basis for more than 2400 hours. Future operational requirements will benefit from the 10 Hz repetition rate of the source, but will require beam pulse length modulation. In the search for higher intensities a number of experiments have been performed on gas composition, RF power matching, extraction, plasma chamber liner and a biased dynode. The results of these tests will be presented.

  4. Experiments on a 14.5 GHz ECR source

    CERN Document Server

    Hill, C E

    1996-01-01

    The 14.5 GHz ECR4 source supplied to CERN in the framework of the Heavy Ion Facility collaboration provided Pb27+ operational beams to a new custom built linac in 1994. This source, which operates in the pulsed "afterglow" mode, quickly met its design specification of 80 emA and now provides currents >100 emA regularly. Early source tests showed the existence of extremely stable modes of operation. In the search for higher intensities a number of experiments have been performed on plasma gas composition, RF power matching, extraction, beam pulse compression and a biased dynode. The results of these tests will be presented along with further ideas to improve source performance

  5. A dysprosium-based metal-organic framework: Synthesis, characterization, crystal structure and interaction with calf thymus-DNA and bovine serum albumin

    Indian Academy of Sciences (India)

    Biplab Mondal; Buddhadeb Sen; Ennio Zangrando; Pabitra Chattopadhyay

    2014-07-01

    A dysprosium-based metallo-organic framework (MOF) containing calcium ions formulated as {Dy(pyda)3Ca1.5(H2O)6} · 5.5H2O (1) (H2pyda = pyridine-2,6-dicarboxylic acid) was solvothermally synthesized in ethanolic medium and characterized by physico-chemical and spectroscopic tools. A detailed structural analysis of the solid state structure of 1 by single crystal X-ray diffraction study showed a tricapped trigonal prism geometry for lanthanide in the [Dy(pyda)3]3− fragment. The mode of interaction of 1 with calf thymus- DNA and with protein bovine serum albumin (BSA) was investigated by using absorption and emission spectroscopic tools. The apparent association constant of complex 1 with CT-DNA was deduced from an absorption spectral study (b = 4.08 × 104 M-1). Spectral and viscosity measurements indicated a groove-binding mode of 1 with CT-DNA, and from spectroscopic study the formation of a metal complex-BSA adduct was assumed to be the result of the interaction of 1 with BSA.

  6. Dual-mode T1 and T2 magnetic resonance imaging contrast agent based on ultrasmall mixed gadolinium-dysprosium oxide nanoparticles: synthesis, characterization, and in vivo application

    Science.gov (United States)

    Tegafaw, Tirusew; Xu, Wenlong; Wasi Ahmad, Md; Baeck, Jong Su; Chang, Yongmin; Bae, Ji Eun; Chae, Kwon Seok; Kim, Tae Jeong; Lee, Gang Ho

    2015-09-01

    A new type of dual-mode T1 and T2 magnetic resonance imaging (MRI) contrast agent based on mixed lanthanide oxide nanoparticles was synthesized. Gd3+ (8S7/2) plays an important role in T1 MRI contrast agents because of its large electron spin magnetic moment resulting from its seven unpaired 4f-electrons, and Dy3+ (6H15/2) has the potential to be used in T2 MRI contrast agents because of its very large total electron magnetic moment: among lanthanide oxide nanoparticles, Dy2O3 nanoparticles have the largest magnetic moments at room temperature. Using these properties of Gd3+ and Dy3+ and their oxide nanoparticles, ultrasmall mixed gadolinium-dysprosium oxide (GDO) nanoparticles were synthesized and their potential to act as a dual-mode T1 and T2 MRI contrast agent was investigated in vitro and in vivo. The D-glucuronic acid coated GDO nanoparticles (davg = 1.0 nm) showed large r1 and r2 values (r2/r1 ≈ 6.6) and as a result clear dose-dependent contrast enhancements in R1 and R2 map images. Finally, the dual-mode imaging capability of the nanoparticles was confirmed by obtaining in vivo T1 and T2 MR images.

  7. Experimental and molecular dynamics studies of dysprosium(III) salt solutions for a better representation of the microscopic features used within the binding mean spherical approximation theory.

    Science.gov (United States)

    Ruas, Alexandre; Guilbaud, Philippe; Den Auwer, Christophe; Moulin, Christophe; Simonin, Jean-Pierre; Turq, Pierre; Moisy, Philippe

    2006-10-19

    This work is aimed at a predictive description of the thermodynamic properties of actinide(III) salt solutions at high concentration and 25 degrees C. A new solution of the binding mean spherical approximation (BIMSA) theory, based on the Wertheim formalism, for taking into account 1:1 and also 1:2 complex formation, is used to reproduce, from a simple procedure, experimental osmotic coefficient variation with concentration for three binary salt solutions of the same lanthanide(III) cation: dysprosium(III) perchlorate, nitrate, and chloride. The relevance of the fitted parameters is discussed, and their values are compared with available literature values. UV-vis/near-IR, time-resolved laser-induced fluorescence spectroscopy experiments, and molecular dynamics (MD) calculations were conducted for dilute to concentrated solutions (ca. 3 mol.kg-1) for a study of the microscopic behavior of DyCl3 binary solutions. Coupling MD calculations and extended X-ray absorption fine structure led to the determination of reliable distances. The MD results were used for a discussion of the parameters used in the BIMSA.

  8. Experimental and molecular dynamics studies of dysprosium(III) salt solutions for a better representation of the microscopic features used within the binding mean spherical approximation theory

    Energy Technology Data Exchange (ETDEWEB)

    Ruas, Alexandre; Guilbaud, Philippe; Den Auwer, Christophe; Moulin, Christophe; Simonin, Jean-Pierre; Turq, Pierre; Moisy, Philippe [DEN/DRCP/SCPS, CEA-Valrho Marcoule, BP 17171, 30207 Bagnols-sur-Ceze Cedex, DEN/DPC/SECR/LSRM, CEA-Saclay, Bat 391, BP 91191 Gif sur Yvette, Cedex (France); Laboratoire LI2C (UMR 7612), Universite Pierre et Marie Curie-Paris 6, Boite No. 51, 4 Place Jussieu, 75252 Paris Cedex 05 (France)

    2006-07-01

    This work is aimed at a predictive description of the thermodynamic properties of actinide (III) salt solutions at high concentration and 25 deg. C. A new solution of the binding mean spherical approximation (BIMSA) theory, based on the Wertheim formalism, for taking into account 1: 1 and also 1: 2 complex formation, is used to reproduce, from a simple procedure, experimental osmotic coefficient variation with concentration for three binary salt solutions of the same lanthanide (III) cation: dysprosium (III) perchlorate, nitrate, and chloride. The relevance of the fitted parameters is discussed, and their values are compared with available literature values. UV-vis/near-IR, time-resolved laser-induced fluorescence spectroscopy experiments, and molecular dynamics (MD) calculations were conducted for dilute to concentrated solutions (ca. 3 mol, kg{sup -1}) for a study of the microscopic behavior of DyCl{sub 3} binary solutions. Coupling MD calculations and extended X-ray absorption fine structure led to the determination of reliable distances. The MD results were used for a discussion of the parameters used in the BIMSA. (authors)

  9. Elucidation of Dual Magnetic Relaxation Processes in Dinuclear Dysprosium(III) Phthalocyaninato Triple-Decker Single-Molecule Magnets Depending on the Octacoordination Geometry.

    Science.gov (United States)

    Katoh, Keiichi; Aizawa, Yu; Morita, Takaumi; Breedlove, Brian K; Yamashita, Masahiro

    2017-08-07

    When applying single-molecule magnets (SMMs) to spintronic devices, control of the quantum tunneling of the magnetization (QTM) as well as a spin-lattice interactions are important. Attempts have been made to use not only coordination geometry but also magnetic interactions between SMMs as an exchange bias. In this manuscript, dinuclear dysprosium(III) (Dy(III) ) SMMs with the same octacoordination geometry undergo dual magnetic relaxation processes at low temperature. In the dinuclear Dy(III) phthalocyaninato (Pc(2-) ) triple-decker type complex [(Pc)Dy(ooPc)Dy(Pc)] (1) (ooPc(2-) =2,3,9,10,16,17,23,24-octakis(octyloxy)phthalocyaninato) with a square-antiprismatic (SAP) geometry, the ground state is divided by the Zeeman effect, and level intersection occurs when a magnetic field is applied. Due to the ground state properties of 1, since the Zeeman diagram where the levels intersect in an Hdc of 2500 Oe, two kinds of QTM and direct processes occur. However, dinuclear Dy(III) -Pc systems with C4 geometry, which have a twist angle (ϕ) of less than 45° do not undergo dual magnetic relaxation processes. From magnetic field and temperature dependences, the dual magnetic relaxation processes were clarified. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Annealing behaviour and crystal structure of RF-sputtered Bi-substituted dysprosium iron-garnet films having excess co-sputtered Bi-oxide content

    Energy Technology Data Exchange (ETDEWEB)

    Vasiliev, M; Nur-E-Alam, M; Alameh, K [Electron Science Research Institute, Edith Cowan University, 270 Joondalup Drive, Joondalup, WA, 6027 (Australia); Premchander, P; Lee, Y T [Department of Information and Communications, Gwangju Institute of Science and Technology (GIST), Gwangju, 500-712 (Korea, Republic of); Kotov, V A [Institute of Radio Engineering and Electronics, Russian Academy of Sciences, 11 Mohovaya St, Moscow, 125009 (Russian Federation); Lee, Y P, E-mail: m.vasiliev@ecu.edu.au [Quantum Photonic Science Research Center, Department of Physics, Hanyang University, 133-791 (Korea, Republic of)

    2011-02-23

    We investigate the magneto-optic properties, crystal structure and annealing behaviour of nano-composite media with record-high magneto-optic quality exceeding the levels reported so far in sputtered iron-garnet films. Bi-substituted dysprosium-gallium iron-garnet films having excess bismuth oxide content are deposited using RF co-sputtering, and a range of garnet materials are crystallized using conventional oven-annealing processes. We report, for the first time ever, the results of optimization of thermal processing regimes for various high-performance magneto-optic iron-garnet compositions synthesized and describe the evolution of the optical and magneto-optical properties of garnet-Bi-oxide composite-material films occurring during the annealing processes. The crystallization temperature boundaries of the system (BiDy){sub 3}(FeGa){sub 5}O{sub 12} : Bi{sub 2}O{sub 3} are presented. We also report the results of x-ray diffraction and energy-dispersive x-ray spectroscopy studies of this recently developed class of high-performance magneto-optic composites. Our hypothesis of iron oxides being the cause of excess optical absorption in sputtered Bi-iron-garnet films is confirmed experimentally.

  11. 26 CFR 1.45D-1 - New markets tax credit.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 1 2010-04-01 2010-04-01 true New markets tax credit. 1.45D-1 Section 1.45D-1... Computing Credit for Investment in Certain Depreciable Property § 1.45D-1 New markets tax credit. (a) Table...) Allowance of credit (1) In general (2) Credit allowance date (3) Applicable percentage (4) Amount paid at...

  12. Downregulation of microRNA-145 promotes epithelial-mesenchymal transition via regulating Snail in osteosarcoma.

    Science.gov (United States)

    Zhang, Z; Zhang, M; Chen, Qinghan; Zhang, Q

    2017-02-01

    Metastasis is the principal cause of cancer death and occurs through multiple, complex processes. Epithelial to mesenchymal transition (EMT) is an important process during embryonic development and has also been hypothesized to exhibit a significant role in cancer cell invasion and metastasis. MicroRNAs (miRNAs) are a class of widespread noncoding RNAs. In recent years, many studies have shown that miRNAs could influence the signaling pathways and downstream events that define EMT on a molecular level. However, the exact role and mechanisms of miR-145 in EMT of osteosarcoma (OS) was unknown. In the present study, miR-145 was downregulated in OS tissues and cell lines and it was shown that miR-145 expression was closely correlated with advanced tumor progression in patients of OS. In addition, miR-145 upregulation by miR-145 agomir significantly inhibited MG63 cells invasion and migration ability. MiR-145 was reported to be able to inhibit EMT in cancers. Following the examination of changes in cell epithelial and mesenchymal markers, it was found that upregulation of miR-145 strongly reversed EMT in MG63 cells. Meanwhile, the expression of Snail, a strong E-cadherin transcription repressor was also attenuated by miR-145 agomir. Furthermore, the decreased EMT and invasion and metastasis caused by miR-145 agomir could be restored by Snail siRNA. In conclusion, the results demonstrated that miR-145 could mediate EMT by targeting Snail and miR-145 might be a novel EMT regulating transcription factor that involved in the progression of OS. The specific drugs targeting miR-145-mediated EMT process might be new promising cancer therapies.

  13. Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP

    DEFF Research Database (Denmark)

    Sheppard, Carol; Blombach, Fabian; Belsom, Adam

    2016-01-01

    initiation, as well as elongation. Both host and viral promoters are subjected to ORF145 repression. Thus, ORF145 has the properties of a global transcription repressor and its overexpression is toxic for Sulfolobus. On the basis of its properties, we have re-named ORF145 RNAP Inhibitory Protein (RIP).......Little is known about how archaeal viruses perturb the transcription machinery of their hosts. Here we provide the first example of an archaeo-viral transcription factor that directly targets the host RNA polymerase (RNAP) and efficiently represses its activity. ORF145 from the temperate Acidianus...

  14. Determination of inositol 1,4,5-trisphosphate levels in Dictyostelium by isotope dilution assay

    Energy Technology Data Exchange (ETDEWEB)

    Van Haastert, P.J.

    1989-02-15

    A commercial isotope dilution assay was used for the determination of Ins(1,4,5)P3 levels in the microorganism Dictyostelium discoideum. Cross-reactivity in the assay was detected with extracts from cells and the medium. The compound which induced this cross-reactivity was tentatively identified as Ins(1,4,5)P3 by (i) codegradation with authentic (/sup 32/P)Ins(1,4,5)P3 by three specific Ins(1,4,5)P3 phosphatases, and (ii) co-chromatography with authentic (/sup 32/P)Ins(1,4,5)P3 on HPLC columns. The cellular concentration was estimated as 165 +/- 42 pmol/10(8) cells, yielding a mean intracellular Ins(1,4,5)P3 concentration of 3.3 microM. Dictyostelium cells secrete large amounts of Ins(1,4,5)P3 at a rate of about 10% of the cellular content per minute, yielding about 0.13 microM extracellular Ins(1,4,5)P3 after 15 min in a suspension of 10(8) cells/ml. The chemoattractant cAMP induced a transient increase of the Ins(1,4,5)P3 concentration; the data suggest an intracacellular rise from 3.3 to 5.5 microM with a maximum at 6 s after stimulation.

  15. MicroRNA-145 suppresses hepatocellular carcinoma by targeting IRS1 and its downstream Akt signaling

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yelin [Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Hu, Chen; Cheng, Jun [Department of Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Chen, Binquan [Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Ke, Qinghong; Lv, Zhen; Wu, Jian [Department of Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Zhou, Yanfeng, E-mail: zyfhdj@yahoo.com [Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China)

    2014-04-18

    Highlights: • MiR-145 expression is down-regulated in HCC tissues and inversely related with IRS1 levels. • MiR-145 directly targets IRS1 in HCC cells. • Restored expression of miR-145 suppressed HCC cell proliferation and growth. • MiR-145 induced IRS1 under-expression potentially reduced downstream AKT signaling. - Abstract: Accumulating evidences have proved that dysregulation of microRNAs (miRNAs) is involved in cancer initiation and progression. In this study, we showed that miRNA-145 level was significantly decreased in hepatocellular cancer (HCC) tissues and cell lines, and its low expression was inversely associated with the abundance of insulin receptor substrate 1 (IRS1), a key mediator in oncogenic insulin-like growth factor (IGF) signaling. We verified IRS1 as a direct target of miR-145 using Western blotting and luciferase reporter assay. Further, the restoration of miR-145 in HCC cell lines suppressed cancer cell growth, owing to down-regulated IRS1 expression and its downstream Akt/FOXO1 signaling. Our results demonstrated that miR-145 could inhibit HCC through targeting IRS1 and its downstream signaling, implicating the loss of miR-145 regulation may be a potential molecular mechanism causing aberrant oncogenic signaling in HCC.

  16. Global use structures of the magnetic materials neodymium and dysprosium. A scenario-based analysis of the effect of the diffusion of electromobility on the demand for rare earths; Globale Verwendungsstrukturen der Magnetwerkstoffe Neodym und Dysprosium. Eine szenariobasierte Analyse der Auswirkung der Diffusion der Elektromobilitaet auf den Bedarf an Seltenen Erden

    Energy Technology Data Exchange (ETDEWEB)

    Gloeser-Chahoud, Simon; Kuehn, Andre; Tercero Espinoza, Luis

    2016-06-15

    Neodymium-iron-boron magnets (NdFeB) have experienced a significant demand as the most powerful permanent magnet in recent years, especially for the manufacture of compact electric servomotors with high efficiency and high power density, especially for mobile applications in hybrid traction motors and electric vehicles or for electric bikes. However, NdFeB magnets are also increasingly being used in general mechanical engineering (conveying and pumping systems, tools, air conditioning systems, lift motors, etc.), in the small electric motors of conventional passenger cars or in the generators of large wind power plants with permanent magnetic direct drive. Nevertheless, there is still high uncertainty in the use structures of NdFeB magnets and the contained rare earth elements neodymium and dysprosium. An effective instrument for increasing the market transparency and the understanding of complex anthropogenic material cycles is the dynamic material flow modeling. In the present work paper, this instrument is used for an in-depth analysis of the use structures of NdFeB magnets and the contained rare earths on a global scale. The dynamic modeling of product usage cycles reveals today's usage structures and quantifies future magnetic quantities in obsolete product flows. It could be shown that the magnets in today's scrap volume are mainly contained in obsolete electronics applications such as hard disks (HDD), CD and DVD drives, which makes the recycling hardly seem to be economical due to the small magnets and the high material spread, but in the foreseeable future with larger magnetic quantities from synchronous servomotors and generators can be expected, which significantly increases the recycling potential. In a further step, the effect of the diffusion of alternative drives in the automotive market on the dysprosium requirement is analyzed using a system dynamics model and possible adaptation mechanisms in the form of different substitution effects in

  17. 21 CFR 1311.145 - Digitally signing the prescription with the individual practitioner's private key.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Digitally signing the prescription with the individual practitioner's private key. 1311.145 Section 1311.145 Food and Drugs DRUG ENFORCEMENT... key may be transmitted to a pharmacy without the digital signature. (f) If the electronic prescription...

  18. 19 CFR 12.145 - Entry or admission of certain steel products.

    Science.gov (United States)

    2010-04-01

    ...: (a) On the entry summary, Customs Form 7501, or on an electronic equivalent, at the time of filing... 19 Customs Duties 1 2010-04-01 2010-04-01 false Entry or admission of certain steel products. 12.145 Section 12.145 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND...

  19. 19 CFR 145.31 - Importations not over $200 in value.

    Science.gov (United States)

    2010-04-01

    ... not over $200 in value. The port director shall pass free of duty and tax, without preparing an entry... 19 Customs Duties 2 2010-04-01 2010-04-01 false Importations not over $200 in value. 145.31 Section 145.31 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND...

  20. 21 CFR 1271.145 - Prevention of the introduction, transmission, or spread of communicable diseases.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Prevention of the introduction, transmission, or spread of communicable diseases. 1271.145 Section 1271.145 Food and Drugs FOOD AND DRUG ADMINISTRATION... FOOD AND DRUG ADMINISTRATION HUMAN CELLS, TISSUES, AND CELLULAR AND TISSUE-BASED PRODUCTS Current Good...

  1. 9 CFR 145.15 - Diagnostic surveillance program for low pathogenic avian influenza.

    Science.gov (United States)

    2010-01-01

    ... low pathogenic avian influenza. 145.15 Section 145.15 Animals and Animal Products ANIMAL AND PLANT... pathogenic avian influenza. (a) The Official State Agency must develop a diagnostic surveillance program for H5/H7 low pathogenic avian influenza for all poultry in the State. The exact provisions of the...

  2. 26 CFR 1.45G-1 - Railroad track maintenance credit.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 1 2010-04-01 2010-04-01 true Railroad track maintenance credit. 1.45G-1... TAXES Rules for Computing Credit for Investment in Certain Depreciable Property § 1.45G-1 Railroad track maintenance credit. (a) In general. For purposes of section 38, the railroad track maintenance credit (RTMC...

  3. 40 CFR 265.145 - Financial assurance for post-closure care.

    Science.gov (United States)

    2010-07-01

    ... care. 265.145 Section 265.145 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... new estimate with the trustee's most recent annual valuation of the trust fund. If the value of the... trust agreement (see § 264.151(a)) -to show current post-closure cost -estimates; (C) Annual...

  4. 9 CFR 145.63 - Terminology and classification; flocks and products.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Terminology and classification; flocks and products. 145.63 Section 145.63 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION... Terminology and classification; flocks and products. Participating flocks, and the eggs and baby...

  5. miR-145 sensitizes gallbladder cancer to cisplatin by regulating multidrug resistance associated protein 1.

    Science.gov (United States)

    Zhan, Ming; Zhao, Xiaonan; Wang, Hui; Chen, Wei; Xu, Sunwang; Wang, Wei; Shen, Hui; Huang, Shuai; Wang, Jian

    2016-08-01

    Gallbladder cancer (GBC) is the most common malignancy in biliary tract with poor prognosis. Due to its high chemoresistance, systemic chemotherapies have had limited success in treating GBC patients. MicroRNAs (miRNAs) are emerging novel regulators of chemoresistance, which modulate the expression of drug resistance-related genes. In this study, we investigated the association between miR-145 expression and cisplatin sensitivity by both in vivo and in vitro analysis. Quantitative PCR (q-PCR) analysis indicated an increased miR-145 expression in GBC tissues. In addition, studies on GBC cell lines suggested an increased cisplatin efficacy with miR-145 overexpression, whereas decreasing miR-145 expression reduced cisplatin sensitivity. Further, we found that miR-145 accelerated MRP1 mRNA degradation by directly targeting its 3'-UTR and therefore caused increased cisplatin toxicity in GBC cells. Moreover, lower miR-145 and higher MRP1 expression levels predicted poor prognosis in GBC patients who received chemotherapy. Collectively, our findings established a rationale for using miR-145 expression as a biomarker to identify cisplatin-resistant GBC patients and propose that treatment strategies increasing the expression of miR-145 could be a new therapeutic approach for GBC patients.

  6. The role of dysprosium on the structural and magnetic properties of (Nd1-xDyx)2Fe14B nanoparticles

    Science.gov (United States)

    Rahimi, Hamed; Ghasemi, Ali; Mozaffarinia, Reza; Tavoosi, Majid

    2017-02-01

    In current work, Nd2Fe14B nanoparticles was synthesized by sol-gel method. Dysprosium powders were added into Nd2Fe14B nanoparticles by mechanical alloying process in order to enhancement of coercivity. The phase analysis, structure, and magnetic properties of annealed (Nd1-xDyx)2Fe14B nanoparticles with different Dy-content (x=0.1, 0.2, 0.3, 0.4, 0.5, 0.6) were investigated by employing X-ray diffraction, X-ray photoelectron spectroscopy, energy dispersive spectroscopy, field emission scanning electron microscope, transmission electron microscope and vibrating sample magnetometer techniques. The results showed that with an increase in Dy amounts, the coercivity of particles increased from 2.9 kOe to 13.4 kOe and then decreased to 5.6 kOe. By adding an optimum amount of Dy (x=0.4), the coercivity was significantly increased from 2.9 kOe to 13.4 kOe. The average particle size of annealed (Nd1-xDyx)2Fe14B nanoparticles was below 10 nm. Magnetization reversal studies indicate that the coercivity of milled and annealed (Nd1-xDyx)2Fe14B nanoparticles is controlled by the nucleation of reversed magnetic domains. The experimental results in the angular dependence of coercivity for (Nd1-xDyx)2Fe14B permanent magnets showed that the normalized coercivity of the permanent magnets Hc(θ)/Hc(0) increases from 1 to about 1.2-1.5 with increasing θ from 0 to about π/3, for x=0.4-0.6.

  7. Management of cholesteatoma complications: Our experience in 145 cases

    Directory of Open Access Journals (Sweden)

    Aziz Mustafa

    2014-01-01

    Full Text Available Objectives: To assess the incidence, clinical features, diagnosis and treatm ent of complications of cholesteatomatous chronic otitis media (CCOM seen in the ENT/Head and Neck Surgery Clinic, University Clinical Center of Kosova, Prishtina. Materials and Methods: This is a retrospective study of the medical records of patients with complications of CCOM who had undergone surgical treatment at the ENT Clinic of the University Clinical Center of Kosovo for the period time of 1994 to 2011. Results: From a total of 2765 patients suffering from COM, 502 (18.08% had cholesteatoma. From this group, in 145 patients had complications. The mean age was 30 years. Eighty-two (56.55% cases had extracranial complications (ECC and 49 patients (33.79% intracranial complications (ICC. For the ECC cases, we found that subperiostal mastoid abscess occurred in 25%, facial nerve palsy was seen in 13% and labyrinthine fistula in 9.6 %. For the ICC cases, meningitis (12% and perisinusal abscess (11% were the most common complications. The most frequent radiological diagnostic procedures were mastoid X-rays, which were performed in 70% of the patients, and computed tomography in 20%. Patients with ECC were treated in the ENT Clinic, whereas patients with ICC, after otologgic surgical procedures, were transferred to the Neurosurgery Clinic. In this series, 5 patients (3.4% died as a result of complications. Conclusions: The incidence of cholesteatoma and its complications in our country still poses a challenge that requires higher dedication. Application of sophisticated diagnostic methods, CT and MRI is going to assist in choosing the adequate surgical approach, especially in cases with intracranial complications

  8. Methylation of miR-145a-5p promoter mediates adipocytes differentiation

    Energy Technology Data Exchange (ETDEWEB)

    Du, Jingjing; Cheng, Xiao; Shen, Linyuan; Tan, Zhendong; Luo, Jia; Wu, Xiaoqian; Liu, Chendong [College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130 (China); Yang, Qiong [Department of Animal Husbandry and Veterinary Medicine, Chengdu Agricultural College, Chengdu 611100, Sichuan (China); Jiang, Yanzhi [College of Life and Science, Sichuan Agricultural University, Chengdu 611130 (China); Tang, Guoqing; Li, Xuewei [College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130 (China); Zhang, Shunhua, E-mail: zhangsh1919@163.com [College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130 (China); Zhu, Li, E-mail: zhuli7508@163.com [College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130 (China)

    2016-06-17

    MicroRNAs (miRNAs, miR) play important roles in adipocyte development. Recent studies showed that the expression of several miRNAs is closely related with promoter methylation. However, it is not known whether miRNA mediates adipocytes differentiation by means of DNA methylation. Here, we showed that miR-145a-5p was poorly expressed in adipose tissue from mice fed a high fat diet (HFD). Overexpression or inhibition of miR-145a-5p was unfavorable or beneficial, respectively, for adipogenesis, and these effects were achieved by regulating adipocyte-specific genes involved in lipogenic transcription, fatty acid synthesis, and fatty acid transportation. Particularly, we first suggested that miR-145a-5p mimics or inhibitors promoted or repressed adipocytes proliferation by regulating p53 and p21, which act as cell cycle regulating factors. Surprisingly, the miR-145a-5p-repressed adipocyte differentiation was enhanced or rescued when cells treated with 5-Aza-dC were transfected with miR-145a-5p mimics or inhibitors, respectively. These data indicated that, as a new mean to positively regulate adipocyte proliferation, the process of miR-145a-5p-inhibited adipogenesis may be regulated by DNA methylation. -- Highlights: •MiR-145a-5p promotes adipocytes proliferation. •MiR-145a-5p is negatively correlated with obesity. •MiR-145a-5p mediates adipocytes differentiation via regulating pathway related adipocytes differentiation. MiR-145a-5p mediating adipocytes differentiation was regulated by DNA methylation.

  9. MiR-145 expression accelerates esophageal adenocarcinoma progression by enhancing cell invasion and anoikis resistance.

    Directory of Open Access Journals (Sweden)

    Mathieu Francois Derouet

    Full Text Available BACKGROUND: Carcinoma of the esophagus has a high case fatality ratio and is now the 6th most common cause of cancer deaths in the world. We previously conducted a study to profile the expression of miRNAs in esophageal adenocarcinoma (EAC pre and post induction therapy. Of the miRNAs differentially expressed post induction chemoradiation, miR-145, a known tumor suppressor miRNA, was upregulated 8-fold following induction therapy, however, its expression was associated with shorter disease-free survival. This unexpected result was explored in this current study. METHODS: In order to study the role of miR-145 in EAC, miRNA-145 was overexpressed in 3 EAC cell lines (OE33, FLO-1, SK-GT-4 and one ESCC cell line (KYSE-410. After validation of the expression of miR-145, hallmarks of cancer such as cell proliferation, resistance to chemotherapy drugs or anoikis, and cell invasion were analyzed. RESULTS: There were no differences in cell proliferation and 5 FU resistance between miR145 cell lines and the control cell lines. miR-145 expression also had no effect on cisplatin resistance in two of three cell lines (OE33 and FLO-1, but miR-145 appeared to protect SK-GT-4 cells against cisplatin treatment. However, there was a significant difference in cell invasion, cell adhesion and resistance to anoikis. All three EAC miR-145 cell lines invaded more than their respective controls. Similarly, OE33 and SK-GT-4 miR-145 cell lines were able to survive longer in a suspension state. DISCUSSION: While expression of miR-145 in ESCC stopped proliferation and invasion, expression of miR-145 in EAC cells enhanced invasion and anoikis resistance. Although more work is required to understand how miR-145 conveys these effects, expression of miR-145 appears to promote EAC progression by enhancing invasion and protection against anoikis, which could in turn facilitate distant metastasis.

  10. Level structure of odd-mass Pr isotopes: Decay of 3. 0-min /sup 145/Ce to levels of /sup 145/Pr

    Energy Technology Data Exchange (ETDEWEB)

    Baum, E.M.; Robertson, J.D.; Mantica P.F. Jr.; Faller, S.H.; Stone, C.A.; Walters, W.B.; Meyer, R.A.; Kusnezov, D.F.

    1989-04-01

    The structure of /sup 145/Pr has been investigated by measuring the gamma-ray singles, gamma-ray coincidence, and gamma-ray angular correlation spectra, along with conversion-electron singles spectra for the decay of 3.0-min /sup 145/Ce to levels of /sup 145/Pr. A level scheme is proposed that includes a number of new levels as well as new spin and parity assignments for several levels. The conversion-coefficient measurements establish the presence of a low-energy (3/2/sup -/) level at 787 keV whose strong population in beta decay confirms negative parity for the ground state of /sup 145/Ce. Interacting boson-fermion model calculations for /sup 143/Pr and /sup 145/Pr are found to be in reasonable agreement with the observed low-energy positive-parity levels, while the presence of the low-energy (3/2/sup -/) level suggests the coexistence of more deformed configurations.

  11. Up-regulated miR-145 expression inhibits porcine preadipocytes differentiation by targeting IRS1.

    Science.gov (United States)

    Guo, Yunxue; Chen, Yaosheng; Zhang, Yun; Zhang, Yue; Chen, Luxi; Mo, Delin

    2012-01-01

    Generally, most miRNAs that were up-regulated during differentiation promoted adipogenesis, but our research indicated that up-regulation of miR-145 in porcine preadipocytes did not promote but inhibit adipogenesis. In this study, miR-145 was significantly up-regulated during porcine dedifferentiated fat (DFAT) cells differentiation. In miR-145 overexpressed DFAT cells, adipogenesis was inhibited and triglycerides accumulation was decreased after hormone stimulation (P<0.05). Furthermore, up-regulation of miR-145 expression repressed induction of mRNA levels of adipogenic markers, such as CCAAT/enhancer-binding protein α (C/EBPα), and peroxisome proliferator-activated receptor γ2 (PPARγ2). These effects caused by miR-145 overexpression were mediated by Insulin receptor substrate 1 (IRS1) as a mechanism. These data suggested that induced miR-145 expression during differentiation could inhibit adipogenesis by targeting IRS1, and miR-145 may be novel agent for adipose tissue engineering.

  12. A Competition Binding Assay for Determination of the Inositol (1,4,5)-Trisphosphate Content of Human Leucocytes

    NARCIS (Netherlands)

    Nibbering, Peter H.; Zomerdijk, Timo P.L.; Haastert, Peter J.M. van; Furth, Ralph van

    1990-01-01

    We developed a competition binding assay for estimation of the intracellular inositol (1,4,5)-trisphosphate (Ins(1,4,5)P3) and optimalized it for the measurement of the Ins(1,4,5)P3 content of human blood leucocytes. The present method is considerably cheaper and requires five times fewer cells than

  13. Two dephosphorylation pathways of inositol 1,4,5-trisphosphate in homogenates of the cellular slime mould Dictyostelium discoideum

    NARCIS (Netherlands)

    Lookeren Campagne, Michiel M. van; Erneux, Cristophe; Eijk, Ronald van; Haastert, Peter J.M. van

    1988-01-01

    Dictyostelium discoideum homogenates contain phosphatase activity which rapidly dephosphorylates Ins(1,4,5)P3 (D-myo-inositol 1,4,5-trisphosphate) to Ins (myo-inositol). When assayed in Mg2+, Ins(1,4,5)P3 is dephosphorylated by the soluble Dictyostelium cell fraction to 20% Ins(1,4)P2 (D-myo-inosito

  14. 36 CFR 14.5 - Nature of interest granted; settlement on right-of-way; rights of ingress and egress.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Nature of interest granted; settlement on right-of-way; rights of ingress and egress. 14.5 Section 14.5 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR RIGHTS-OF-WAY Nature of Interest § 14.5 Nature...

  15. 26 CFR 145.4051-1 - Imposition of tax on heavy trucks and trailers sold at retail.

    Science.gov (United States)

    2010-04-01

    ... sold at retail. 145.4051-1 Section 145.4051-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF... trailers sold at retail. (a) Imposition of tax—(1) In general. Section 4051(a)(1) imposes a tax on the first retail sale (as defined in § 145.4052-1(a)) of the following articles (including in each...

  16. TEM-145 and TEM-146 β-lactamases produced by Escherichia coli ...

    African Journals Online (AJOL)

    GREGO

    2007-03-05

    Mar 5, 2007 ... responsible for their resistance to β-lactamase inhibitors. The isolates were ... molecular modifications introduced to overcome this resistance .... following changes compared to TEM-1: TEM-145 had two changes; L221M and ...

  17. Coulomb excitation of levels in /sup 143/Nd and /sup 145/Nd

    Energy Technology Data Exchange (ETDEWEB)

    Dragulescu, E.; Ivascu, M.; Mihu, R.; Popescu, D.; Semenescu, G.; Paar, V.; Vretenar, D.

    1984-04-30

    The low-lying states of /sup 143/Nd and /sup 154/Nd have been studied by means of Coulomb excitation with /sup 16/O and ..cap alpha..-particles. Angular distribution measurements were carried out for some transitions in /sup 145/Nd with 11.2 MeV ..cap alpha..-particles. Level energy decay schemes and B(E2)up values were measured for two states in /sup 143/Nd and for six states in /sup 145/Nd. Some spin assignments have been established for the /sup 145/Nd nucleus. /sup 143/Nd and /sup 145/Nd have been theoretically described by coupling one and three particles, respectively, to quadrupole vibrations, and rather good agreement with experiment was achieved. 36 references.

  18. Dysprosium(III) complexes with a square-antiprism configuration featuring mononuclear single-molecule magnetic behaviours based on different β-diketonate ligands and auxiliary ligands.

    Science.gov (United States)

    Zhang, Sheng; Ke, Hongshan; Shi, Quan; Zhang, Jangwei; Yang, Qi; Wei, Qing; Xie, Gang; Wang, Wenyuan; Yang, Desuo; Chen, Sanping

    2016-03-28

    Three mononuclear dysprosium(III) complexes derived from three β-diketonate ligands, 4,4,4-trifluoro-1-(4-methylphenyl)-1,3-butanedione (tfmb), 4,4,4-trifluoro-1-(4-fluorophenyl)-1,3-butanedione (tffb) and 4,4,4-trifluoro-1-(2-naphthyl)-1,3-butanedione (tfnb) as well as auxiliary ligands, 5-nitro-1,10-phenanthroline (5-NO2-Phen), DMF and 2,2'-bipyridine (bpy) have been synthesized and structurally characterized, namely [Dy(5-NO2-Phen)(tfmb)3] (1), [Dy(DMF)2(tffb)3] (2) and [Dy(bpy)2(tfnb)3]·0.5(1,4-dioxane) (3). The metal ions in 1-3 adopt an approximately square-antiprismatic (SAP) coordination environment with D4d axial symmetry. The magnetic properties of 1-3 have been investigated, displaying weak out-of-phase AC signals under a zero-DC field. With an applied DC field of 1200 Oe, the quantum tunnelling of the magnetization was suppressed in 1-3 with the pre-exponential factor τ0 = 5.3 × 10(-7) s and the effective barrier ΔE/kB = 83 K for 1 as well as the pre-exponential factor τ0 = 3.09 × 10(-7) s and the effective barrier ΔE/kB = 39 K for 3. Interestingly, for the frequency dependence of the out-of-phase (χ'') of the AC susceptibility of 2, two slow relaxation of the magnetization processes occurred under the applied magnetic field of 1200 Oe, corresponding to the fast relaxation (FR) phase and slow relaxation (SR) phase, respectively. Arrhenius analysis gave the effective energy barrier (ΔE/kB) of 55 K and the pre-exponential factor (τ0) of 8.23 × 10(-12) for the SR. It is thus very likely that the FR process in complex 2 results from QTM enhanced by dipolar interactions between the Dy ions or the presence of the applied field. The structure-property relationship of some Dy(III) based mononuclear SMMs with the SAP configuration was further discussed.

  19. Magnetic Phase Transition of Nanocrystalline Bulk Metal Gadolinium and Dysprosium%纳米块体金属钆和镝的磁性相变分析

    Institute of Scientific and Technical Information of China (English)

    刘凤艳; 侯碧辉; 岳明; 王克军

    2011-01-01

    The magnetic properties of bulk nanocrystalline metal gadolinium (Gd) and dysprosium (Dy)samples were studied.The magnetization and Curie temperature TC of nanocrystalline Gd and Dy decreased usually as compared with the polycrystal.However,when the mean grain size was 10 nm, the Curie temperature Tc of nanocrystalline Dy increased to 100 K instead and there was an antiferromagnetic phase in nanocrystalline Gd.According to the calculation based on Ruderman-Kittel-Kasuya-Yosida exchange interaction, the exchange integral of the grain boundary atoms and crystalline surface atoms had its sign changed from plus to minus or vice versa, and there were three orderly phases in the steady state with the lowest energy, ferromagnetic phase, antiferromagnetic phase and fan phase.For the nanocrystals with mean grain size of 10 nm, the proportion of grain boundary to crystalline surface atoms was high, and as the result of superposition of the three phases, and there appeared a peak near the phase transition temperature for the nanocrystalline Gd.While for the Dy, the magnetization decreased gently with temperature, and showing a higher Curie temperature than in the case of the polycrystal.%对纳米晶钆(Gd)和镝(Dy)块体材料的磁性进行了研究.与多晶比较,通常纳米晶的磁化强度减小,居里温度TC降低,但平均粒径为10 nm的纳米晶Dy的居里温度TC反而升高到100 K,平均粒径为10 nm的纳米晶Gd中还存在明显的反铁磁相.通过RKKY交换作用的计算知道,晶面晶界处原子的交换积分会发生正负号的变化,能量最低的稳定状态对应三种有序相:铁磁相、反铁磁相和扇相,晶粒中在一定条件下出现三相共存.对于平均粒径为10 nm的纳米晶,晶面晶界处原子所占比例很大,三相叠加的结果,对于Gd,即是在相变点附近出现磁化强度尖峰;对于Dy,则是磁化强度随温度升高下降缓慢,表现为居里温度TC比多晶升高.

  20. CALIX[4]ARENE C-145 EFFECTS ON СELLULAR HAEMOSTASIS

    Directory of Open Access Journals (Sweden)

    V. O. Chernyshenko

    2016-06-01

    Full Text Available The aim of the research was to study a potential antithrombotic sodium salt of calix[4]arene-methylene-bis-phosphonic acid (С-145 — on activation and aggregation of platelets in vivo, as well as on proliferation and apoptosis of endothelial cells in the cell culture. Effects of calix[4]arene С-145 estimated in vitro after addition to the platelet rich plasma, and in vivo after intravenous injection into rabbit bloodstream in equivalent amounts (46 μM. Aggregation of platelets was induced by adenosine diphosphate and detected using aggregometer Solar AP2110. Platelet shape and cytoplasmic granularity were monitored on COULTER EPICS XL Flow Cytometer. The level of tissuetype plasminogen activator — tPA — was estimated using enzyme-linked immunosorbent assay ELISA. Effects of calix[4]arene C-145 on culture of endotelial cells cells was studied using 3-(4,5-Dimethylthiazol-2-yl-2,5-Diphenyltetrazolium Bromide — MTT-test. The population of proliferative pool of cells (G2/M+S was determined using flow cytometry. Aggregometry and flow cytometry showed that calix[4]arene C-145 did not activate platelets nor affect their aggregation in vitro. However intravenous injection of calix[4]arene C-145 into the bloodstream of healthy rabbits leads to strong inhibition of platelet aggregation and changes of shape and granularity of most of the platelets after 2 hours of administration. Any additional appearance of endothelial cells activation marker tPA in vivo and any inhibition of calix[4]arene C-145 on proliferation of endothelial cells in cell culture did not observe. So calix[4]arene C-145 had strong anti-platelet effect in vivo that was not a result of their direct action on platelets or endothelial cells in vitro. This allowed to assume the possibility of calix[4]arene C-145 use as an effective antithrombotic agent.

  1. Optimization of Subculture Conditions for Marc-145 Cells%Marc-145细胞传代培养条件优化

    Institute of Scientific and Technical Information of China (English)

    赵潇; 秦钢

    2013-01-01

    Using single test,the effect of recovery temperature serum concentration,growth solution pH value,incubation temperature,concentration of digestive fluid and passage rate on the subculture for Marc-145 cells was studied.The results showed that the best subculture conditions for Marc-145 cells was when the recovery temperature was 37℃,the serum concentration was 10%,growth solution pH value was 7.2,incubation temperature was 37℃,concentration of digestive fluid was 0.25%,and passage rate was 1∶3.%采用单因素法研究在细胞传代培养过程中复苏温度、血清浓度、生长液pH值、培养温度、消化液浓度、传代比例等因素对Marc-145细胞传代培养的影响。结果表明:复苏温度37℃、血清浓度10%、生长液pH值7.2、培养温度37℃、消化液浓度0.25%、传代比例1∶3时Marc-145细胞传代培养效果最佳。

  2. Human cytomegalovirus UL145 gene is highly conserved among clinical strains

    Indian Academy of Sciences (India)

    Zhengrong Sun; Ying Lu; Qiang Ruan; Yaohua Ji; Rong He; Ying Qi; Yanping Ma; Yujing Huang

    2007-09-01

    Human cytomegalovirus (HCMV), a ubiquitous human pathogen, is the leading cause of birth defects in newborns. A region (referred to as UL/b′) present in the Toledo strain of HCMV and low-passage clinical isolates) contains 22 additional genes, which are absent in the highly passaged laboratory strain AD169. One of these genes, UL145 open reading frame (ORF), is located between the highly variable genes UL144 and UL146. To assess the structure of the UL145 gene, the UL145 ORF was amplified by PCR and sequenced from 16 low-passage clinical isolates and 15 non-passage strains from suspected congenitally infected infants. Nine UL145 sequences previously published in the GenBank were used for sequence comparison. The identities of the gene and the similarities of its putative protein among all strains were 95.9–100% and 96.6–100%, respectively. The post-translational modification motifs of the UL145 putative protein in clinical strains were conserved, comprising the protein kinase C phosphorylation motif (PKC) and casein kinase II phosphorylation site (CK-II). We conclude that the structure of the UL145 gene and its putative protein are relatively conserved among clinical strains, irrespective of whether the strains come from patients with different manifestations, from different areas of the world, or were passaged or not in human embryonic lung fibroblast (HELF) cells.

  3. Differential expression of speckled POZ protein, SPOP: Putative regulation by miR-145

    Indian Academy of Sciences (India)

    Chiu-Jung Huang; Hsing-Yu Chen; Wan-Yi Lin; Kongbung Choo

    2014-06-01

    The speckle POZ protein, SPOP, is an adaptor of the Cul3-based ubiquitination process, and has been implicated in the carcinogenesis process. Despite recent elucidation of biological functions, regulation of SPOP gene expression has not been reported. In this study, the mRNA levels of the mouse SPOP (mSPOP) gene were first shown to vary noticeably in different tissues. However, the SPOP protein was detected in high abundance only in Purkinje cells of the cerebellum and seminiferous tubule of the testis, echoing previous reports of involvement of ubiquitination in neuron cells and in spermatogenesis. In other mouse tissues and human cancer cell lines analysed, only low SPOP protein levels were detected. The 3′-untranslated regions of both the mSPOP and human SPOP transcripts harbor a conserved putative miR-145 binding site (BS). In some tissues and cell lines, miR-145 and SPOP protein levels were in an inverse relationship suggesting miR-145 regulation. Luciferase assays of deletion and point mutation constructs of the miR-145 BS, and miR-145 induction by serum starvation that resulted in reduced endogenous SPOP levels provided further evidence that miR-145 is likely involved in post-transcriptional regulation of SPOP expression in selected tissues, and possibly with the participation of other miRNA species.

  4. Structures of the autoproteolytic domain from the Saccharomyces cerevisiae nuclear pore complex component, Nup145

    Energy Technology Data Exchange (ETDEWEB)

    Sampathkumar, Parthasarathy; Ozyurt, Sinem A.; Do, Johnny; Bain, Kevin T.; Dickey, Mark; Rodgers, Logan A.; Gheyi, Tarun; Sali, Andrej; Kim, Seung Joong; Phillips, Jeremy; Pieper, Ursula; Fernandez-Martinez, Javier; Franke, Josef D.; Martel, Anne; Tsuruta, Hiro; Atwell, Shane; Thompson, Devon A.; Emtage, J. Spencer; Wasserman, Stephen R.; Rout, Michael P.; Sauder, J. Michael; Burley, Stephen K. (SLAC); (Rockefeller); (UCSF); (Lilly)

    2012-04-30

    Nuclear pore complexes (NPCs) are large, octagonally symmetric dynamic macromolecular assemblies responsible for exchange of proteins and RNAs between the nucleus and cytoplasm. NPCs are made up of at least 456 polypeptides from {approx}30 distinct nucleoporins. Several of these components, sharing similar structural motifs, form stable subcomplexes that form a coaxial structure containing two outer rings (the nuclear and cytoplasmic rings), two inner rings, and a membrane ring. The yeast (Saccharomyces cerevisiae) Nup145 and its human counterpart are unique among the nucleoporins, in that they undergo autoproteolysis to generate functionally distinct proteins. The human counterpart of Nup145 is expressed as two alternatively spliced mRNA transcripts. The larger 190 kDa precursor undergoes post-translational autoproteolysis at the Phe863-Ser864 peptide bond yielding the 92 kDa Nup98 and the 96 kDa Nup96. The smaller 98 kDa precursor is also autoproteolysed at an analogous site giving 92 kDa Nup98-N and a 6 kDa C-terminal fragment, which may form a noncovalent complex. The yeast Nup145 precursor [Fig. 1(A)] contains twelve repeats of a 'GLFG' peptide motif (FG repeats) at its N-terminus, an internal autoproteolytic domain (a region of high conservation with the homologous yeast nucleoporins Nup110 and Nup116, neither of which undergo autoproteolysis), followed by the C-terminal domain. Various forms of the FG repeats are present in nearly half of all nucleoporins; they form intrinsically disordered regions implicated in gating mechanisms that control passage of macromolecules through NPCs. Nup145 undergoes autoproteolysis at the Phe605-Ser606 peptide bond to generate two functionally distinct proteins, Nup145N and Nup145C. Subsequently, Nup145C associates with six other proteins to form the heptameric Y-complex, a component of the outer rings of the NPC. Nup145N, on the other hand, can shuttle between the NPC and the nuclear interior. It has been suggested

  5. Sequence Variations in the Flagellar Antigen Genes fliCH25 and fliCH28 of Escherichia coli and Their Use in Identification and Characterization of Enterohemorrhagic E. coli (EHEC O145:H25 and O145:H28.

    Directory of Open Access Journals (Sweden)

    Lothar Beutin

    Full Text Available Enterohemorrhagic E. coli (EHEC serogroup O145 is regarded as one of the major EHEC serogroups involved in severe infections in humans. EHEC O145 encompasses motile and non-motile strains of serotypes O145:H25 and O145:H28. Sequencing the fliC-genes associated with the flagellar antigens H25 and H28 revealed the genetic diversity of the fliCH25 and fliCH28 gene sequences in E. coli. Based on allele discrimination of these fliC-genes real-time PCR tests were designed for identification of EHEC O145:H25 and O145:H28. The fliCH25 genes present in O145:H25 were found to be very similar to those present in E. coli serogroups O2, O100, O165, O172 and O177 pointing to their common evolution but were different from fliCH25 genes of a multiple number of other E. coli serotypes. In a similar way, EHEC O145:H28 harbor a characteristic fliCH28 allele which, apart from EHEC O145:H28, was only found in enteropathogenic (EPEC O28:H28 strains that shared some common traits with EHEC O145:H28. The real time PCR-assays targeting these fliCH25[O145] and fliCH28[O145] alleles allow better characterization of EHEC O145:H25 and EHEC O145:H28. Evaluation of these PCR assays in spiked ready-to eat salad samples resulted in specific detection of both types of EHEC O145 strains even when low spiking levels of 1-10 cfu/g were used. Furthermore these PCR assays allowed identification of non-motile E. coli strains which are serologically not typable for their H-antigens. The combined use of O-antigen genotyping (O145wzy and detection of the respective fliCH25[O145] and fliCH28[O145] allele types contributes to improve identification and molecular serotyping of E. coli O145 isolates.

  6. Sequence Variations in the Flagellar Antigen Genes fliCH25 and fliCH28 of Escherichia coli and Their Use in Identification and Characterization of Enterohemorrhagic E. coli (EHEC) O145:H25 and O145:H28

    Science.gov (United States)

    Beutin, Lothar; Delannoy, Sabine; Fach, Patrick

    2015-01-01

    Enterohemorrhagic E. coli (EHEC) serogroup O145 is regarded as one of the major EHEC serogroups involved in severe infections in humans. EHEC O145 encompasses motile and non-motile strains of serotypes O145:H25 and O145:H28. Sequencing the fliC-genes associated with the flagellar antigens H25 and H28 revealed the genetic diversity of the fliCH25 and fliCH28 gene sequences in E. coli. Based on allele discrimination of these fliC-genes real-time PCR tests were designed for identification of EHEC O145:H25 and O145:H28. The fliCH25 genes present in O145:H25 were found to be very similar to those present in E. coli serogroups O2, O100, O165, O172 and O177 pointing to their common evolution but were different from fliCH25 genes of a multiple number of other E. coli serotypes. In a similar way, EHEC O145:H28 harbor a characteristic fliCH28 allele which, apart from EHEC O145:H28, was only found in enteropathogenic (EPEC) O28:H28 strains that shared some common traits with EHEC O145:H28. The real time PCR-assays targeting these fliCH25[O145] and fliCH28[O145] alleles allow better characterization of EHEC O145:H25 and EHEC O145:H28. Evaluation of these PCR assays in spiked ready-to eat salad samples resulted in specific detection of both types of EHEC O145 strains even when low spiking levels of 1–10 cfu/g were used. Furthermore these PCR assays allowed identification of non-motile E. coli strains which are serologically not typable for their H-antigens. The combined use of O-antigen genotyping (O145wzy) and detection of the respective fliCH25[O145] and fliCH28[O145] allele types contributes to improve identification and molecular serotyping of E. coli O145 isolates. PMID:26000885

  7. Sequence Variations in the Flagellar Antigen Genes fliCH25 and fliCH28 of Escherichia coli and Their Use in Identification and Characterization of Enterohemorrhagic E. coli (EHEC) O145:H25 and O145:H28.

    Science.gov (United States)

    Beutin, Lothar; Delannoy, Sabine; Fach, Patrick

    2015-01-01

    Enterohemorrhagic E. coli (EHEC) serogroup O145 is regarded as one of the major EHEC serogroups involved in severe infections in humans. EHEC O145 encompasses motile and non-motile strains of serotypes O145:H25 and O145:H28. Sequencing the fliC-genes associated with the flagellar antigens H25 and H28 revealed the genetic diversity of the fliCH25 and fliCH28 gene sequences in E. coli. Based on allele discrimination of these fliC-genes real-time PCR tests were designed for identification of EHEC O145:H25 and O145:H28. The fliCH25 genes present in O145:H25 were found to be very similar to those present in E. coli serogroups O2, O100, O165, O172 and O177 pointing to their common evolution but were different from fliCH25 genes of a multiple number of other E. coli serotypes. In a similar way, EHEC O145:H28 harbor a characteristic fliCH28 allele which, apart from EHEC O145:H28, was only found in enteropathogenic (EPEC) O28:H28 strains that shared some common traits with EHEC O145:H28. The real time PCR-assays targeting these fliCH25[O145] and fliCH28[O145] alleles allow better characterization of EHEC O145:H25 and EHEC O145:H28. Evaluation of these PCR assays in spiked ready-to eat salad samples resulted in specific detection of both types of EHEC O145 strains even when low spiking levels of 1-10 cfu/g were used. Furthermore these PCR assays allowed identification of non-motile E. coli strains which are serologically not typable for their H-antigens. The combined use of O-antigen genotyping (O145wzy) and detection of the respective fliCH25[O145] and fliCH28[O145] allele types contributes to improve identification and molecular serotyping of E. coli O145 isolates.

  8. Construction and Expression of a Eukaryotic Vector for miR- 145%miR-145真核表达载体的构建及表达

    Institute of Scientific and Technical Information of China (English)

    谢海涛

    2012-01-01

    Objective To construct a eukaryotic expression vector for miR - 145, so as to lay a foundation for exploring its biological function in colon cancer. Methods The gene fragment of miR - 145 was obtained by PCR and inserted into a eukaryotic expression plasmid, pCMV - myc. The constructed plasmid, pCMV -miR~ 145, was transfected into the colon cancer cell line HCT116. The expression level of miR - 145 was detected by real - time PCR. Results The successful cloning of miR- 145 into the eukaryotic expression vector pCMV-myc was confirmed by enzyme digestion and DNA sequencing analysis. The reconstructed plasmid could significantly increase the expression of miR -145 in HCT116 cells. Conclusions A eukaryotic expression vector for miR -145, pCMV - miR - 145, is successfully constructed. It can highly express miR -145.%目的 构建miR- 145的真核表达载体,为研究miR- 145在结肠癌中的生物学功能奠定基础.方法 设计并应用PCR扩增miR- 145基因片段,将其导入真核表达载体pCMV- myc中构建重组质粒pCMV - miR - 145后,将重组质粒转染入结肠癌细胞系HCT116中,运用RT- PCR检测miR - 145的表达情况.结果 酶切及DNA测序证实miR- 145被正确克隆入真核表达载体pCMV- myc中,该重组质粒能在HCT- 116细胞中高效表达miR- 145.结论 成功构建了miR- 145的真核表达载体pCMV- miR - 145,该载体能有效高表达miR- 145.

  9. Hepatitis B surface gene 145 mutant as a minor population in hepatitis B virus carriers

    Directory of Open Access Journals (Sweden)

    Komatsu Haruki

    2012-01-01

    Full Text Available Abstract Background Hepatitis B virus (HBV can have mutations that include the a determinant, which causes breakthrough infection. In particular, a single mutation at amino acid 145 of the surface protein (G145 is frequently reported in the failure of prophylactic treatment. The aim of this study was to evaluate the frequency of the a determinant mutants, especially the G145 variant, in Japan, where universal vaccination has not been adopted. Methods The present study was a retrospective study. The study cohorts were defined as follows: group 1, children with failure to prevent mother-to-child transmission despite immunoprophylaxis (n = 18, male/female = 8/10, age 1-14 years; median 6 years; group 2, HBV carriers who had not received vaccination or hepatitis B immunoglobulin (n = 107, male/female = 107, age 1-52 years; median 16 years. To detect the G145R and G145A mutants in patients, we designed 3 probes for real-time PCR. We also performed direct sequencing and cloning of PCR products. Results By mutant-specific real-time PCR, one subject (5.6% was positive for the G145R mutant in group 1, while the G145 mutant was undetectable in group 2. The a determinant mutants were detected in one (5.6% of the group 1 subjects and 10 (9.3% of the group 2 subjects using direct sequencing, but direct sequencing did not reveal the G145 mutant as a predominant strain in the two groups. However, the subject who was positive according to the mutant-specific real-time PCR in group 1 had overlapped peaks at nt 587 in the electropherogram. In group 2, 11 patients had overlapped peaks at nt 587 in the electropherogram. Cloning of PCR products allowed detection of the G145R mutant as a minor strain in 7 (group 1: 1 subject, group 2: 6 subjects of 12 subjects who had overlapped peaks at nt 587 in the electropherogram. Conclusions The frequency of the a determinant mutants was not high in Japan. However, the G145R mutant was often present as a minor population in

  10. Extension of the energy range of the experimental activation cross-sections data of longer-lived products of proton induced nuclear reactions on dysprosium up to 65 MeV

    CERN Document Server

    Tárkányi, F; Takács, S; Hermanne, A; Ignatyuk, A V

    2016-01-01

    Activation cross-sections data of longer-lived products of proton induced nuclear reactions on dysprosium were extended up to 65 MeV by using stacked foil irradiation and gamma spectrometry experimental methods. Experimental cross-sections data for the formation of the radionuclides $^{159}$Dy, $^{157}$Dy, $^{155}$Dy, $^{161}$Tb, $^{160}$Tb, $^{156}$Tb, $^{155}$Tb, $^{154m2}$Tb, $^{154m1}$Tb, $^{154g}$Tb, $^{153}$Tb, $^{152}$Tb and $^{151}$Tb are reported in the 36-65 MeV energy range, and compared with an old dataset from 1964. The experimental data were also compared with the results of cross section calculations of the ALICE and EMPIRE nuclear model codes and of the TALYS nuclear reaction model code as listed in the latest on-line libraries TENDL 2013.

  11. Extension of the energy range of the experimental activation cross-sections data of longer-lived products of proton induced nuclear reactions on dysprosium up to 65MeV.

    Science.gov (United States)

    Tárkányi, F; Ditrói, F; Takács, S; Hermanne, A; Ignatyuk, A V

    2015-04-01

    Activation cross-sections data of longer-lived products of proton induced nuclear reactions on dysprosium were extended up to 65MeV by using stacked foil irradiation and gamma spectrometry experimental methods. Experimental cross-sections data for the formation of the radionuclides (159)Dy, (157)Dy, (155)Dy, (161)Tb, (160)Tb, (156)Tb, (155)Tb, (154m2)Tb, (154m1)Tb, (154g)Tb, (153)Tb, (152)Tb and (151)Tb are reported in the 36-65MeV energy range, and compared with an old dataset from 1964. The experimental data were also compared with the results of cross section calculations of the ALICE and EMPIRE nuclear model codes and of the TALYS nuclear reaction model code as listed in the latest on-line libraries TENDL 2013. Copyright © 2015. Published by Elsevier Ltd.

  12. MiR-145 regulates epithelial to mesenchymal transition of breast cancer cells by targeting Oct4.

    Directory of Open Access Journals (Sweden)

    Jiajia Hu

    Full Text Available MiR-145 could regulate tumor growth, apoptosis, migration, and invasion. In our present study, we investigated its role in epithelial-mesenchymal transition (EMT. Expression of miR-145 was decreased in breast tumor tissues at T3&4 stages in comparison with those at T1&2. Over-expression of miR-145 mimics enhanced protein levels of E-cadherin and dampened those of α-SMA and Fibronectin, indicative of its inhibitory role in EMT occurrence. Mechanistic studies showed that miR-145 mimics inhibited Oct4 expression and miR-145 inhibitor enhanced it. Over-expression of Oct4 reversed miR-145-regulated expression of EMT markers, suggesting that Oct4 mediated the inhibitory effects of miR-145. MiR-145 could inhibite the expression of Snail, ZEB1, and ZEB2, while over-expression of Oct4 rescued the effects. Furthermore, Oct-4 induced over-expression of transcription factor Snail, ZEB1 and ZEB2 was mediated by β-catenin. Expression of Slug and Twist were not altered by miR-145/Oct4. Taken together, our results have revealed a novel role of miR-145 on EMT. It inhibits EMT by blocking the expression of Oct4, and downstream transcriptional factors, Snail, ZEB1 and ZEB2.

  13. MicroRNA-145 targets YES and STAT1 in colon cancer cells

    DEFF Research Database (Denmark)

    Gregersen, Lea H; Jacobsen, Anders B; Frankel, Lisa

    2010-01-01

    miRNA overexpression. Gene Ontology analysis showed an overrepresentation of genes involved in cell death, cellular growth and proliferation, cell cycle, gene expression and cancer. A number of the identified miRNA targets have previously been implicated in cancer, including YES, FSCN1, ADAM17, BIRC2......, VANGL1 as well as the transcription factor STAT1. Both YES and STAT1 were verified as direct miR-145 targets. CONCLUSIONS/SIGNIFICANCE: The study identifies and validates new cancer-relevant direct targets of miR-145 in colon cancer cells and hereby adds important mechanistic understanding of the tumor......BACKGROUND: MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in tumorigenesis. miR-145 is reported to be down-regulated in several cancers, but knowledge of its targets in colon cancer remains limited. METHODOLOGY/PRINCIPAL FINDINGS: To investigate...

  14. [The role of micro-RNA/143/145 in evolution of intra-stent restenosis].

    Science.gov (United States)

    Popovich, I M

    2011-01-01

    The mechanisms of neointima formation and hyperplasia in restenosis remain non-elucidated yet. Because micro-ribonucleic acids/143/145(micro-RNA/143 and micro-RNA/145) participate in the regulation and sustaining of the genotype of mature vascular myocytes we have measured their expression in tissue content of restenoses taken postmortem from 5 patients who underwent angioplasty and subsequently died, and studied its association with actin quantity and fibrillar collagen type I degradation degree. It has been found that during restenosis progression quantity of micro-RNA/143 and micro-RNA/145 decreases in media and intima of coronary artery. This finding has been associated with appearance in coronary intima of coronary myocytes with reduced size likely of secretory phenotype, diminution of number of myocytes with contractile phenotype, and increase of quantity of denaturized collagen type I-phenomena characteristic for neointima hyperplasia, a substrate of intra-stent restenosis.

  15. Effect of a triaxial nuclear shape on proton tunneling: the decay and structure of 145Tm.

    Science.gov (United States)

    Seweryniak, D; Blank, B; Carpenter, M P; Davids, C N; Davinson, T; Freeman, S J; Hammond, N; Hoteling, N; Janssens, R V F; Khoo, T L; Liu, Z; Mukherjee, G; Robinson, A; Scholey, C; Sinha, S; Shergur, J; Starosta, K; Walters, W B; Woehr, A; Woods, P J

    2007-08-24

    Gamma rays deexciting states in the proton emitter 145Tm were observed using the recoil-decay tagging method. The 145Tm ground-state rotational band was found to exhibit the properties expected for an h{11/2} proton decoupled band. In addition, coincidences between protons feeding the 2{+} state in 144Er and the 2{+}-->0{+} gamma-ray transition were detected, the first measurement of this kind, leading to a more precise value for the 2{+} excitation energy of 329(1) keV. Calculations with the particle-rotor model and the core quasiparticle coupling model indicate that the properties of the pi{11/2} band and the proton-decay rates in 145Tm are consistent with the presence of triaxiality with an asymmetry parameter gamma approximately 20 degrees .

  16. SarA influences the sporulation and secondary metabolism in Streptomyces coelicolor M145

    Institute of Scientific and Technical Information of China (English)

    Xijun Ou; Bo Zhang; Lin Zhang; Kai Dong; Chun Liu; Guoping Zhao; Xiaoming Ding

    2008-01-01

    The filamentous bacteria Streptomyces exhibit a complex life cycle involving morphological differentiation and secondary metabolism. A putative membrane protein gene sarA (sco4069), sporulation and antibiotic production related gene A, was partially characterized in Streptomyces coelicolor M145. The gene product had no characterized functional domains and was highly conserved in Streptomyces. Compared with the wild-type M145, the sarA mutant accelerated sporulation and dramatically decreased the production of actinorhodin and undecylprodigiosin.Reverse transcription-polymerase chain reaction analysis showed that SarA influenced antibiotic production by controlling the abundance of actll-orf4 and redZ messenger RNA.

  17. Dysprosium selective potentiometric membrane sensor

    Energy Technology Data Exchange (ETDEWEB)

    Zamani, Hassan Ali, E-mail: haszamani@yahoo.com [Department of Applied Chemistry, Mashhad Branch, Islamic Azad University, Mashhad (Iran, Islamic Republic of); Faridbod, Farnoush; Ganjali, Mohammad Reza [Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran (Iran, Islamic Republic of)

    2013-03-01

    A novel Dy(III) ion-selective PVC membrane sensor was made using a new synthesized organic compound, 3,4-diamino-N Prime -((pyridin-2-yl)methylene)benzohydrazide (L) as an excellent sensing element. The electrode showed a Nernstian slope of 19.8 {+-} 0.6 mV per decade in a wide concentration range of 1.0 Multiplication-Sign 10{sup -6}-1.0 Multiplication-Sign 10{sup -2} mol L{sup -1}, a detection limit of 5.5 Multiplication-Sign 10{sup -7} mol L{sup -1}, a short conditioning time, a fast response time (< 10 s), and high selectivity towards Dy(III) ion in contrast to other cations. The proposed sensor was successfully used as an indicator electrode in the potentiometric titration of Dy(III) ions with EDTA. The membrane sensor was also applied to the F{sup -} ion indirect determination of some mouth washing solutions and to the Dy{sup 3+} determination in binary mixtures. Highlights: Black-Right-Pointing-Pointer The novelty of this work is based on the high affinity of the ionophore toward the Dy{sup 3+} ions. Black-Right-Pointing-Pointer This technique is very simple, fast and inexpensive and it is not necessary to use sophisticated equipment. Black-Right-Pointing-Pointer The newly developed sensor is superior to the formerly reported Dy{sup 3+} sensors in terms of selectivity.

  18. The magnetocaloric effect in dysprosium

    Science.gov (United States)

    Benford, S. M.

    1979-01-01

    The magnetocaloric effect in polycrystalline Dy was measured in the 84-280-K range in measuring fields from 1 to 7 T. These adiabatic temperature changes reflect structural changes in Dy with applied field and temperature, and include the first magnetocaloric data for a helical antiferromagnet. Above the Neel point (179 K) a field increase always caused heating; below the Neel point fields less than about 2 T cause cooling for some values of initial temperature. The largest temperature increase with a 7 T field occurs at the Neel point and at fields below 2 T near the Curie point. For refrigeration purposes the optimal working region for a Dy cooling element is field dependent.

  19. MicroRNA-145 targets vascular endothelial growth factor and inhibits invasion and metastasis of osteosarcoma cells

    Institute of Scientific and Technical Information of China (English)

    Lei Fan; Qiang Wu; Xiaojuan Xing; Yulong Wei; Zengwu Shao

    2012-01-01

    MicroRNAs are important gene regulators that play a profound role in tumorigenesis.MicroRNA-145 (miR-145),an important member in the family of microRNAs,is underexpressed in several types of tumors and acts as a tumor suppressor.The role and probable pathways of miR-145in osteosarcoma carcinogenesis are still unknown.In this study,we found that miR-145 was significantly underexpressed in osteosarcoma tissues,and the over-expression of miR-145 could inhibit invasion and angiopoiesis of osteosarcoma cells.Furthermore,the results showed that vascular endothelial growth factor (VEGF) expression was down-regulated in osteosarcoma cells after miR-145 transfection.On the basis of these results,we performed the luciferase assay and verified that miR-145 could downregulate VEGF at the translational level by partially binding to VEGF 3' untranslated region (3'UTR).Therefore,it can be concluded that miR-145 can inhibit invasion and metastasis of osteosarcoma cells.One of the mechanisms is the down-regulation of VEGF expression by miR-145 by binding to the 3'UTR of VEGF mRNA specifically.These novel findings may have extensive implications for an effective gene therapy of osteosarcoma.

  20. 14 CFR 382.145 - What records concerning training must carriers retain?

    Science.gov (United States)

    2010-01-01

    ... retain for three years individual employee training records demonstrating that all persons required to... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false What records concerning training must... TRAVEL Training and Administrative Provisions § 382.145 What records concerning training must...

  1. 42 CFR 84.145 - Motor-operated blower test; minimum requirements.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Motor-operated blower test; minimum requirements... Supplied-Air Respirators § 84.145 Motor-operated blower test; minimum requirements. (a) Motor-operated... connection between the motor and the blower shall be so constructed that the motor may be disengaged from...

  2. 42 CFR 456.145 - Number of studies required to be performed.

    Science.gov (United States)

    2010-10-01

    ...: Medical Care Evaluation Studies § 456.145 Number of studies required to be performed. The hospital must, at least, have one study in progress at any time and complete one study each calendar year. ... 42 Public Health 4 2010-10-01 2010-10-01 false Number of studies required to be performed....

  3. 5 CFR 185.145 - Deposit in Treasury of the United States.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Deposit in Treasury of the United States... REGULATIONS PROGRAM FRAUD CIVIL REMEDIES § 185.145 Deposit in Treasury of the United States. All amounts collected pursuant to this part shall be deposited as miscellaneous receipts in the Treasury of the...

  4. Limits on fast radio bursts at 145 MHz with ARTEMIS, a real-time software backend

    NARCIS (Netherlands)

    A. Karastergiou; J. Chennamangalam; W. Armour; C. Williams; B. Mort; F. Dulwich; S. Salvini; A. Magro; S. Roberts; M. Serylak; A. Doo; A.V. Bilous; R.P. Breton; H. Falcke; J.M. Grießmeier; J.W.T. Hessels; E.F. Keane; V.I. Kondratiev; M. Kramer; J. van Leeuwen; A. Noutsos; S. Osłowski; C. Sobey; B.W. Stappers; P. Weltevrede

    2015-01-01

    Fast radio bursts (FRBs) are millisecond radio signals that exhibit dispersion larger than what the Galactic electron density can account for. We have conducted a 1446 h survey for FRBs at 145 MHz, covering a total of 4193 deg2 on the sky. We used the UK station of the low frequency array (LOFAR) ra

  5. 26 CFR 1.145-2 - Application of private activity bond regulations.

    Science.gov (United States)

    2010-04-01

    ... of the private business use test and the private security or payment test mean “5 percent” and “net... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Application of private activity bond regulations... Bonds § 1.145-2 Application of private activity bond regulations. (a) In general. Except as provided...

  6. 9 CFR 145.23 - Terminology and classification; flocks and products.

    Science.gov (United States)

    2010-01-01

    ... SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Multiplier Egg-Type Chicken Breeding Flocks and Products § 145.23... breeding chickens through routine serological surveillance of each participating breeding flock. A flock...

  7. 9 CFR 145.33 - Terminology and classification; flocks and products.

    Science.gov (United States)

    2010-01-01

    ... SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Multiplier Meat-Type Chicken Breeding Flocks and Products § 145.33... influenza in primary breeding chickens through routine serological surveillance of each participating...

  8. 9 CFR 145.73 - Terminology and classification; flocks and products.

    Science.gov (United States)

    2010-01-01

    ... SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Primary Egg-Type Chicken Breeding Flocks and Products § 145.73 Terminology... presence of avian influenza in primary breeding chickens through routine serological surveillance of each...

  9. 9 CFR 145.83 - Terminology and classification; flocks and products.

    Science.gov (United States)

    2010-01-01

    ... POULTRY Special Provisions for Primary Meat-Type Chicken Breeding Flocks and Products § 145.83 Terminology... for primary meat-type breeders wishing to assure their customers that the chicks produced are... flock collected and cultured as described in § 147.12(a)(5) of this subchapter; or (B) A sample of chick...

  10. 46 CFR 171.145 - Drainage of a vessel with a cockpit.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Drainage of a vessel with a cockpit. 171.145 Section 171... vessel with a cockpit. (a) Except as follows, the cockpit must be watertight: (1) A cockpit may have companionways if they comply with § 171.124(d). (2) A cockpit may have ventilation openings along its...

  11. 40 CFR 81.145 - State Capital Intrastate Air Quality Control Region.

    Science.gov (United States)

    2010-07-01

    ... Quality Control Regions § 81.145 State Capital Intrastate Air Quality Control Region. The State Capital Intrastate Air Quality Control Region (Virginia) consists of the territorial area encompassed by the... 40 Protection of Environment 17 2010-07-01 2010-07-01 false State Capital Intrastate Air...

  12. 20 CFR 404.145 - When you acquire a quarter of coverage.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false When you acquire a quarter of coverage. 404... DISABILITY INSURANCE (1950- ) Insured Status and Quarters of Coverage Quarters of Coverage § 404.145 When you acquire a quarter of coverage. If we credit you with a quarter of coverage (QC) for a calendar...

  13. Determination of lnositol 1,4,5-Trisphosphate Levels in Dictyostelium by Isotope Dilution Assay

    NARCIS (Netherlands)

    Haastert, Peter J.M. van

    1989-01-01

    A commercial isotope dilution assay was used for the determination of Ins(1,4,5)P3 levels in the microorganism Dictyostelium discoideum. Cross-reactivity in the assay was detected with extracts from cells and the medium. The compound which induced this cross-reactivity was tentatively identified as

  14. 22 CFR 145.20 - Purpose of financial and program management.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Purpose of financial and program management... INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management § 145.20 Purpose of financial and program management. Sections...

  15. 40 CFR 40.145-2 - Federal Water Pollution Control Act.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Federal Water Pollution Control Act. 40... FEDERAL ASSISTANCE RESEARCH AND DEMONSTRATION GRANTS § 40.145-2 Federal Water Pollution Control Act. (a... or control of acid or other mine water pollution; and (2) That the State shall provide legal...

  16. 14 CFR 145.105 - Change of location, housing, or facilities.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Change of location, housing, or facilities..., Materials, and Data § 145.105 Change of location, housing, or facilities. (a) A certificated repair station... certificated repair station must operate while it is changing its location, housing, or facilities. ...

  17. miR Cluster 143/145 Directly Targets Nrl and Regulates Rod Photoreceptor Development.

    Science.gov (United States)

    Sreekanth, Sreekumaran; Rasheed, Vazhanthodi A; Soundararajan, Lalitha; Antony, Jayesh; Saikia, Minakshi; Sivakumar, Krishnankutty Chandrika; Das, Ani V

    2016-11-23

    Retinal histogenesis requires coordinated and temporal functioning of factors by which different cell types are generated from multipotent progenitors. Development of rod photoreceptors is regulated by multiple transcription factors, and Nrl is one of the major factors involved in their fate specification. Presence or absence of Nrl at the postnatal stages decides the generation of cone photoreceptors or other later retinal cells. This suggests the need for regulated expression of Nrl in order to accelerate the generation of other cell types during retinal development. We found that miR cluster 143/145, comprising miR-143 and miR-145, targets and imparts a posttranscriptional inhibition of Nrl. Expression of both miRNAs was differentially regulated during retinal development and showed least expression at PN1 stage in which most of the rod photoreceptors are generated. Downregulation of rod photoreceptor regulators and markers upon miR cluster 143/145 overexpression demonstrated that this cluster indeed negatively regulates rod photoreceptors. Further, we prove that Nrl positively regulates miR cluster 143/145, thus establishing a feedback loop regulatory mechanism. This may be one possible mechanism by which Nrl is posttranscriptionally regulated to facilitate the generation of other cell types in retina.

  18. GE-145, a new low-osmolar dimeric radiographic contrast medium

    Energy Technology Data Exchange (ETDEWEB)

    Wistrand, Lars-G.; Rogstad, Astri; Hagelin, Gunnar (GE Healthcare AS, Medical Diagnostics, Nydalen, Oslo (Norway)), e-mail: lars-goeran.wistrand@ge.com (and others)

    2010-11-15

    Background: Contrast-induced nephrotoxicity is a significant risk when using radiographic contrast media clinically, especially in high risk patients. Consequently, there is a need for a new contrast agent with improved clinical safety with regards to nephrotoxicity. Purpose: To evaluate the physicochemical properties as well as the preclinical safety and biodistribution parameters of the newly developed radiographic contrast medium GE-145. Material and Methods: Standard methods for radiographic contrast media were used for evaluation of physicochemical properties. The acute toxicity in rats was studied at 8, 10, and 12.5 gI/kg, the clinical chemistry parameters were determined, and histology of the kidneys was performed. Biodistribution was studied in rats using 123I-labeled GE-145. Results: GE-145 is more hydrophilic than iodixanol and has a considerably lower osmolality. The viscosity is similar to that of iodixanol and the protein binding is low. The acute toxicity is similar to that of iodixanol and the biodistribution is similar to that of other radiographic contrast media, showing mainly renal excretion. Kidney histology showed a moderate reversible vacuolization, similar to that of iodixanol. Conclusion: GE-145 exhibits similar preclinical properties to other dimeric radiographic contrast media. In addition, the low osmolality enables an iso-osmolar formulation containing a significantly higher concentration of electrolytes than Visipaque

  19. Anthocyanin Induces Apoptosis of DU-145 Cells In Vitro and Inhibits Xenograft Growth of Prostate Cancer

    Science.gov (United States)

    Ha, U-Syn; Bae, Woong Jin; Kim, Su Jin; Yoon, Byung Il; Hong, Sung Hoo; Lee, Ji Youl; Hwang, Tae-Kon; Hwang, Sung Yeoun; Wang, Zhiping

    2015-01-01

    Purpose To investigate the effects of anthocyanins extracted from black soybean, which have antioxidant activity, on apoptosis in vitro (in hormone refractory prostate cancer cells) and on tumor growth in vivo (in athymic nude mouse xenograft model). Materials and Methods The growth and viability of DU-145 cells treated with anthocyanins were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and apoptosis was assessed by DNA laddering. Immunoblotting was conducted to evaluate differences in the expressions of p53, Bax, Bcl, androgen receptor (AR), and prostate specific antigen (PSA). To study the inhibitory effects of anthocyanins on tumor growth in vivo, DU-145 tumor xenografts were established in athymic nude mice. The anthocyanin group was treated with daily oral anthocyanin (8 mg/kg) for 14 weeks. After 2 weeks of treatment, DU-145 cells (2×106) were inoculated subcutaneously into the right flank to establish tumor xenografts. Tumor dimensions were measured twice a week using calipers and volumes were calculated. Results Anthocyanin treatment of DU-145 cells resulted in 1) significant increase in apoptosis in a dose-dependent manner, 2) significant decrease in p53 and Bcl-2 expressions (with increased Bax expression), and 3) significant decrease in PSA and AR expressions. In the xenograft model, anthocyanin treatment significantly inhibit tumor growth. Conclusion This study suggests that anthocyanins from black soybean inhibit the progression of prostate cancer in vitro and in a xenograft model. PMID:25510742

  20. 42 CFR 433.145 - Assignment of rights to benefits-State plan requirements.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Assignment of rights to benefits-State plan... Liability Assignment of Rights to Benefits § 433.145 Assignment of rights to benefits—State plan..., cooperation in establishing paternity and obtaining support, and cooperation in identifying and...

  1. 76 FR 52871 - Pseudomonas fluorescens Strain CL145A; Exemption From the Requirement of a Tolerance

    Science.gov (United States)

    2011-08-24

    ... AGENCY 40 CFR Part 180 Pseudomonas fluorescens Strain CL145A; Exemption From the Requirement of a... establishes an exemption from the requirement of a tolerance for residues of Pseudomonas fluorescens strain... eliminates the need to establish a maximum permissible level for residues of Pseudomonas fluorescens...

  2. 40 CFR 63.145 - Process wastewater provisions-test methods and procedures to determine compliance.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 9 2010-07-01 2010-07-01 false Process wastewater provisions-test... Operations, and Wastewater § 63.145 Process wastewater provisions—test methods and procedures to determine... analytical method for wastewater which has that compound as a target analyte. (7) Treatment using a series...

  3. Clinicopathological Significance of microRNA-31, -143 and -145 Expression in Colorectal Cancer

    Directory of Open Access Journals (Sweden)

    Chao-Jie Wang

    2009-01-01

    Full Text Available We are just beginning to understand how microRNAs (miRNAs are involved in tumor-related processes in humans. Applying real-time RT-PCR, we investigated the miR-31, miR-143 and miR-145 expression in 98 primary CRC specimens, along with the corresponding normal mucosa specimens, and analyze the relationship of their expression with clinicopathological features. Our results showed the miR-31 expression was up-regulated in CRC compared to normal mucosa (p = 0.001. Furthermore, miR-31 expression was positively related to advanced TNM stage (p = 0.026 and deeper invasion of tumors (p = 0.024. MiR-145 was down-regulated in both colon (p = 0.001 and rectal (p = 0.012 cancer. MiR-143 was only down-regulated in colon cancer (p = 0.023 but not in rectal cancer (p = 0.351. There was no relationship of miR-143 and miR-145 expression with other clinicopathological features (p > 0.05, except that the miR-145 expression was related to cancer site (p = 0.03. In conclusion, the miR-31 overexpression may be involved in the development and progression of CRC. The miR-143 and miR-145 may play a certain role in the development of colon and/or rectal cancers but not in progression of the disease.

  4. MiR-145 regulates PAK4 via the MAPK pathway and exhibits an antitumor effect in human colon cells

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Zhigang [Department of General Surgery, Shanghai Jiaotong University Affiliated 6th People' s Hospital, Shanghai (China); Zhang, Xiaoping [Department of Nuclear Medicine, Shanghai 10th People' s Hospital, Tongji University School of Medicine (China); Yang, Zhili; Du, Hangxiang; Wu, Zhenqian; Gong, Jianfeng; Yan, Jun [Department of General Surgery, Shanghai Jiaotong University Affiliated 6th People' s Hospital, Shanghai (China); Zheng, Qi, E-mail: zhengqi1957@yahoo.com.cn [Department of General Surgery, Shanghai Jiaotong University Affiliated 6th People' s Hospital, Shanghai (China)

    2012-10-26

    Highlights: Black-Right-Pointing-Pointer MiR-145 targets a putative binding site in the 3 Prime UTR of PAK4. Black-Right-Pointing-Pointer MiR-145 played an important role in inhibiting cell growth by directly targeting PAK4. Black-Right-Pointing-Pointer MiR-145 may function as tumor suppressors. -- Abstract: MicroRNAs (miRNAs) are regulators of numerous cellular events; accumulating evidence indicates that miRNAs play a key role in a wide range of biological functions, such as cellular proliferation, differentiation, and apoptosis in cancer. Down-regulated expression of miR-145 has been reported in colon cancer tissues and cell lines. The molecular mechanisms underlying miR-145 and the regulation of colon carcinogenesis remain unclear. In this study, we investigated the levels of miR-145 in human colon cancer cells using qRT-PCR and found markedly decreased levels compared to normal epithelial cells. We identified PAK4 as a novel target of miR-145 using informatics screening. Additionally, we demonstrated that miR-145 targets a putative binding site in the 3 Prime UTR of PAK4 and that its abundance is inversely associated with miR-145 expression in colon cancer cells; we confirmed this relationship using the luciferase reporter assay. Furthermore, restoration of miR-145 by mimics in SW620 cells significantly attenuated cell growth in vitro, in accordance with the inhibitory effects induced by siRNA mediated knockdown of PAK4. Taken together, these findings demonstrate that miR-145 downregulates P-ERK expression by targeting PAK4 and leads to inhibition of tumor growth.

  5. MicroRNA‑145 inhibits the malignant phenotypes of gastric carcinoma cells via downregulation of fascin 1 expression.

    Science.gov (United States)

    Xue, Minghui; Zhao, Lunde; Yang, Fang; Li, Zhenjuan; Li, Guangyan

    2016-01-01

    MicroRNA (miR)‑145 has been demonstrated to act as a tumor suppressor, and deregulation of fascin 1 (FSCN1) has been observed in several types of human malignancy, including gastric carcinoma. However, the molecular mechanism underlying the function of miR‑145, specifically its targets in gastric carcinoma have yet to be fully elucidated. In the present study, downregulation of miR‑145 and upregulation of FSCN1 was identified in gastric carcinoma cell lines, compared with normal gastric mucosal epithelial cells. A luciferase reporter assay demonstrated that miR‑145 was able to bind to the 3'‑untranslated region of FSCN1 mRNA. Overexpression of miR‑145 led to a significant decrease in FSCN1 expression levels, whereas knockdown of miR‑145 resulted in increased FSCN1 expression levels in gastric carcinoma cells. Furthermore, overexpression of miR‑145 inhibited proliferation, migration and invasion in gastric carcinoma cells. Similar effects were also observed in gastric carcinoma cells transfected with FSCN1 small interfering RNA. In addition, overexpression of FSCN1 reversed the suppressive effects of miR‑145 upregulation on proliferation, migration and invasion in gastric carcinoma cells, suggesting that FSCN1 is indeed involved in the miR‑145‑mediated malignant phenotype of gastric carcinoma cells. The present study revealed an anti‑oncogenic role of miR‑145 in gastric carcinoma via inhibition of FSCN1, and suggested that miR‑145 may be used for the treatment of gastric carcinoma.

  6. Visualization of Ins(1,4,5)P3 dynamics in living cells: two distinct pathways for Ins(1,4,5)P3 generation following mechanical stimulation of HSY-EA1 cells.

    Science.gov (United States)

    Nezu, Akihiro; Tanimura, Akihiko; Morita, Takao; Tojyo, Yosuke

    2010-07-01

    In the present study, the contribution of inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3)] generation on the mechanical-stimulation-induced Ca(2+) response was investigated in HSY-EA1 cells. Mechanical stimulation induced a local increase in the cytosolic concentration of Ins(1,4,5)P(3) ([IP(3)](i)), as indicated by the Ins(1,4,5)P(3) biosensor LIBRAvIII. The area of this increase expanded like an intracellular Ins(1,4,5)P(3) wave as [IP(3)](i) increased in the stimulated region. A small transient [IP(3)](i) increase was subsequently seen in neighboring cells. The phospholipase C inhibitor U-73122 abolished these Ins(1,4,5)P(3) responses and resultant Ca(2+) releases. The purinergic receptor blocker suramin completely blocked increases in [IP(3)](1) and the Ca(2+) release in neighboring cells, but failed to attenuate the responses in mechanically stimulated cells. These results indicate that generation of Ins(1,4,5)P(3) in response to mechanical stimulation is primarily independent of extracellular ATP. The speed of the mechanical-stimulation-induced [IP(3)](i) increase was much more rapid than that induced by a supramaximal concentration of ATP (1 mM). The contribution of the Ins(1,4,5)P(3)-induced Ca(2+) release was larger than that of Ca(2+) entry in the Ca(2+) response to mechanical stimulation in HSY-EA1 cells.

  7. T145

    Directory of Open Access Journals (Sweden)

    G.Ch. Sim

    2015-11-01

    Full Text Available High dose IL-2 (HD IL-2 has been extensively used as an immunotherapy against metastatic melanoma However, why HD IL-2 is effective only in a subset of patients and whether predictive biomarkers, before or early during the course of therapy, can be used to improve response rates remain unresolved. In addition, it has been found that IL-2 therapy potently expands CD4+CD25+Foxp3+ T-regulatory cells (Tregs but how Treg cell levels, phenotype, and function change and whether specific subsets of Tregs are activated and expanded during HD IL-2 therapy is remain unclear. In this study, we performed comprehensive multi-parameter FACS analysis of patient blood before and two days after the last bolus of IL-2 infusion during cycle 1 of HD IL-2 therapy. Two lymphocyte subsets were found to expand the most during the first cycle of IL-2 therapy: CD4+CD25+Foxp3+ Tregs expressing an activation marker, inducible costimulator (ICOS, and CD3-CD56hiCD16loPerforin+ NK cells. ICOS+ Tregs expressed significantly higher levels of CD25, Foxp3 and had a more activated phenotype than ICOS− Tregs as indicated by lower levels of CD45RA and CD127 expression. Further phenotypic characterization revealed a more suppressive phenotype on ICOS+ Treg with higher expression levels of CD39, CD73, and TGF-β/LAP than ICOS− Treg. ICOS+ Tregs were also the predominant Treg cells that secreted IL-10 and have potent T-cell suppressor function. Majority of ICOS+ Tregs from HD IL-2-treated patients were Ki67+ and exhibited an enhanced proliferative response to IL-2 ex vivo relative to ICOS− Tregs. Functional analysis revealed that ICOS+ Tregs secreted little IFN- and IL-2 in comparison to CD4+Foxp3 – cells. Furthermore, analysis on 38 IL-2-treated patients at MD Anderson, we found that non-responders had a significantly higher degree of ICOS+ Treg expansion than responders during the first cycle of IL-2 therapy, while no significant changes in the ICOS− or bulk Treg population. In conclusion, our data suggests that tracking changes in ICOS+ Tregs early during the course of HD IL-2 therapy may be a new predictive biomarker of clinical outcome.

  8. 9 CFR 381.145 - Poultry products and other articles entering or at official establishments; examination and other...

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Poultry products and other articles entering or at official establishments; examination and other requirements. 381.145 Section 381.145 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND...

  9. Dephosphorylation Pathway of D-myo-Inositol 1,4,5-trisphosphate in the Unicellular Green Alga Chlamydomonas eugametos

    NARCIS (Netherlands)

    Klerk, Hans; Himbergen, John A.J. van; Musgrave, Alan; Haastert, Peter J.M. van; Ende, Herman van den

    1994-01-01

    In vitro dephosphorylation of D-myo-inositol 1,4,5-trisphosphate [Ins(l,4,5)P-3] by vegetative cells, gametes and zygotes of the green alga Chlamydomonas eugametos was studied using a soluble cell fraction as enzyme source and labelled Ins(1,4,5)P-3 as substrate. This compound was dephosphorylated y

  10. Differential expression of miR-145 in children with Kawasaki disease.

    Directory of Open Access Journals (Sweden)

    Chisato Shimizu

    Full Text Available BACKGROUND: Kawasaki disease is an acute, self-limited vasculitis of childhood that can result in structural damage to the coronary arteries. Previous studies have implicated the TGF-β pathway in disease pathogenesis and generation of myofibroblasts in the arterial wall. microRNAs are small non-coding RNAs that modulate gene expression at the post-transcriptional level and can be transported between cells in extracellular vesicles. To understand the role that microRNAs play in modifying gene expression in Kawasaki disease, we studied microRNAs from whole blood during the acute and convalescent stages of the illness. METHODOLOGY/PRINCIPAL FINDINGS: RNA isolated from the matched whole blood of 12 patients with acute and convalescent Kawasaki disease were analyzed by sequencing of small RNA. This analysis revealed six microRNAs (miRs-143, -199b-5p, -618, -223, -145 and -145* (complementary strand whose levels were significantly elevated during the acute phase of Kawasaki disease. The result was validated using targeted qRT-PCR using an independent cohort (n = 16. miR-145, which plays a critical role in the differentiation of neutrophils and vascular smooth muscle cells, was expressed at high levels in blood samples from acute Kawasaki disease but not adenovirus-infected control patients (p = 0.005. miR-145 was also detected in small extracellular vesicles isolated from acute Kawasaki disease plasma samples. Pathway analysis of the predicted targets of the 6 differentially expressed microRNAs identified the TGF-β pathway as the top pathway regulated by microRNAs in Kawasaki disease. CONCLUSION: Sequencing of small RNA species allowed discovery of microRNAs that may participate in Kawasaki disease pathogenesis. miR-145 may participate, along with other differentially expressed microRNAs, in regulating expression of genes in the TGF-β pathway during the acute illness. If the predicted target genes are confirmed, our findings suggest a model of

  11. MicroRNA-145 Regulates Neural Stem Cell Differentiation Through the Sox2-Lin28/let-7 Signaling Pathway.

    Science.gov (United States)

    Morgado, Ana L; Rodrigues, Cecília M P; Solá, Susana

    2016-05-01

    MicroRNAs (miRNAs or miRs) regulate several biological functions, including cell fate determination and differentiation. Although miR-145 has already been described to regulate glioma development, its precise role in neurogenesis has never been addressed. miR-145 represses sex-determining region Y-box 2 (Sox2), a core transcription factor of embryonic stem cells (ESCs), to inhibit pluripotency and self-renewal in human ESCs. In addition, the Sox2-Lin28/let-7 signaling pathway regulates proliferation and neurogenesis of neural precursors. In this study, we aimed to investigate the precise role of miR-145 in neural stem cell (NSC) fate decision, and the possible involvement of the Sox2-Lin28/let-7 signaling pathway in miR-145 regulatory network. Our results show for the first time that miR-145 expression significantly increased after induction of mouse NSC differentiation, remaining elevated throughout this process. Forced miR-145 downregulation decreased neuronal markers, namely βIII-tubulin, NeuN, and MAP2. Interestingly, throughout NSC differentiation, protein levels of Sox2 and Lin28, a well-known suppressor of let-7 biogenesis, decreased. Of note, neuronal differentiation also resulted in let-7a and let-7b upregulation. Transfection of NSCs with anti-miR-145, in turn, increased both Sox2 and Lin28 protein levels, while decreasing both let-7a and let-7b. More importantly, Sox2 and Lin28 silencing partially rescued the impairment of neuronal differentiation induced by miR-145 downregulation. In conclusion, our results demonstrate a novel role for miR-145 during NSC differentiation, where miR-145 modulation of Sox2-Lin28/let-7 network is crucial for neurogenesis progression. Stem Cells 2016;34:1386-1395. © 2016 AlphaMed Press.

  12. 牛人捉到Pokemon游戏中145只精灵

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    《Pokemon Go》游戏如今正风靡全球,美国电玩达人尼克一约翰逊展示他捕获到的145只精灵。根据《Pokemon Go》游戏原著,目前在《Pokemon Go》中的精灵共有151种。

  13. A qualitative phenomenological study: Enhanced, risk-based FAA oversight on part 145 maintenance practices

    Science.gov (United States)

    Sheehan, Bryan G.

    The purpose of this qualitative phenomenological study was to examine the phenomenon of enhanced, risk-based Federal Aviation Administration (FAA) oversight of Part 145 repair stations that performed aircraft maintenance for Part 121 air carriers between 2007 and 2014 in Oklahoma. Specifically, this research was utilized to explore what operational changes have occurred in the domestic Part 145 repair station industry such as variations in management or hiring practices, training, recordkeeping and technical data, inventory and aircraft parts supply-chain logistics, equipment, and facilities. After interviewing 12 managers from Part 145 repair stations in Oklahoma, six major theme codes emerged from the data: quality of oversight before 2007, quality of oversight after 2007, advantages of oversight, disadvantages of oversight, status quo of oversight, and process improvement . Of those six major theme codes, 17 subthemes appeared from the data that were used to explain the phenomenon of enhanced oversight in the Part 145 repair station industry. Forty-two percent of the participants indicated a weak FAA oversight system that has hindered the continuous process improvement program in their repair stations. Some of them were financially burdened after hiring additional full-time quality assurance inspectors to specifically manage enhanced FAA oversight. Notwithstanding, the participants of the study indicated that the FAA must apply its surveillance on a more standardized and consistent basis. They want to see this standardization in how FAA inspectors interpret regulations and practice the same quality of oversight for all repair stations, particularly those that are repeat violators and fail to comply with federal aviation regulations. They believed that when the FAA enforces standardization on a consistent basis, repair stations can become more efficient and safer in the performance of their scope of work for the U.S. commercial air transportation industry.

  14. milk-145通过下调OCT4基因仰制肺腺癌干细胞增殖%milk-145 Inhibits Lung Adenocarcinoma Stem Cells Proliferation by Targeting OCT4 Gene

    Institute of Scientific and Technical Information of China (English)

    张帅; 武雅琴; 冯冬杰; 张治; 蒋峰; 尹荣; 许林

    2011-01-01

    Background and objective MiR-l45 functions as a protective miRNA identified in tumor tissues of lung adenocarcinoma patients.The aim of this study is to investigate the relationship between miR-145 and proliferation of lung cancer stem cells and involved molecular mechanisms in human lung adenocarcinaoma A549 cell line.Methods MicroRNA microarray technology was conducted to compare miRNA signature between tumor and adjacent normal tissue of lung adenocaranaoma.The potential target gene of miR-l45 was predicted by online bioinformatic softwares.Pre-miR- 145 mimics and anti-miR-145 inhibitor were transfected into A549 cell line by lipofectamine 2000.miR-l45 expression in each group was detected by real time PCR.The OCT4 protein level was analyzed by Western blot.The predicted miR-l45 binding site in OCT4 3'-untranslated region (UTR) was validated by dual-luciferase reporter gene assay.CCK-8 assay was employed to observe the proliferation activity of A549 cells.The ratio of CDl33 positive cells in each group was analyzed by flow cytometry.Results miR-l45 expression was significantly down-regulated in lung adenocarcinoma compared with ajacent normal tissue.OCT4 is a potential target gene of miR-l45 predicted by miRanda.Compared with control group, miR-l45 was significantly up-regulated and down-regulated in the pre-miR-145 mimics and anti-miR-l45 inhibitor groups respectively Overexpression of miR-145 inhibited the proliferation of A549 cells.Both the OCT4 protein level and CD133 positive ratio were remarkably decreased in the pre-miR-145 mimics group, whereas significantly increased in the anti-miR-145 inhibitor group.Dual-luciferase reporter gene assay validated the predicted miR-145 binding site of OCT4 3'UTR.Conclusion MiR-145 can inhibit the proliferation of lung cancer stem cells in A549 cell line via down-regulating O CT4 expression.MiR-145 is a potential protective miRNA of lung cancer.%背景与目的 milk-145是通过miRNA芯片及qPCR验证筛选出的一

  15. A Comparative Study on Proteomics between LNCap and DU145 Cells by Quantitative Detection and SELDI Analysis

    Institute of Scientific and Technical Information of China (English)

    Weigui SUN; Zhangqun YE; Zhenguo MI; Tianliang SHI; Cunzhi HAN; Sutang GUO

    2008-01-01

    The differences in intracellular and extracellular protein expressions between human prostate cancer fines LNCap and DU145 were examined. The proteins of the two cell lines were extracted and condensed by using protein extraction kits. And the intracellular and extracellular proteins were quantitatively detected on a micro-plate reader by using bicinchoninie acid (BCA) method. The proteins in cell culture fluid were qualitatively assayed by SELDI-TOF-MS. The results showed that the intracellular protein contents of LNCap cells were extremely higher than those of DU145 cells. After serum-free culture, both intracellular and extracellular protein contents of LNCap and DU145 were decreased to some extent. And the intracellular proteins were decreased by 5% in LNCap and by 36% in DU145 respectively, while the extracellular proteins were decreased by 89% in LNCap and 96% in DU145 respectively. SELDI assay revealed that there were 5 marker proteins in LNCap and 6 in DU145. Although both LNCap and DU145 cell lines originated from human prostate cancer, they had some differences in protein expression.

  16. MicroRNA-145 restores contractile vascular smooth muscle phenotype and coronary collateral growth in the metabolic syndrome.

    Science.gov (United States)

    Hutcheson, Rebecca; Terry, Russell; Chaplin, Jennifer; Smith, Erika; Musiyenko, Alla; Russell, James C; Lincoln, Thomas; Rocic, Petra

    2013-04-01

    Transient, repetitive occlusion stimulates coronary collateral growth (CCG) in normal animals. Vascular smooth muscle cells (VSMCs) switch to synthetic phenotype early in CCG, then return to contractile phenotype. CCG is impaired in the metabolic syndrome. We determined whether impaired CCG was attributable to aberrant VSMC phenotypic modulation by miR-145-mediated mechanisms, and whether restoration of physiological miR-145 levels in metabolic syndrome (JCR rat) improved CCG. CCG was stimulated by transient, repetitive left anterior descending artery occlusion and evaluated after 9 days by coronary blood flow measurements (microspheres). miR-145 was delivered to JCR VSMCs via adenoviral vector (miR-145-Adv). In JCR rats, miR-145 was decreased late in CCG (≈ 2-fold day 6; ≈ 4-fold day 9 versus SD), which correlated with decreased expression of smooth muscle-specific contractile proteins (≈ 5-fold day 6; ≈ 10-fold day 9 versus SD), indicative of VSMCs' failure to return to the contractile phenotype late in CCG. miR-145 expression in JCR rats (miR-145-Adv) on days 6 to 9 of CCG completely restored VSMCs contractile phenotype and CCG (collateral/normal zone flow ratio was 0.93 ± 0.09 JCR+miR-145-Adv versus 0.12 ± 0.02 JCR versus 0.87 ± 0.02 SD). Restoration of VSMC contractile phenotype through miR-145 delivery is a highly promising intervention for restoration of CCG in the metabolic syndrome.

  17. The role of miR-145 in stem cell characteristics of human laryngeal squamous cell carcinoma Hep-2 cells.

    Science.gov (United States)

    Karatas, Omer Faruk; Suer, Ilknur; Yuceturk, Betul; Yilmaz, Mehmet; Hajiyev, Yusif; Creighton, Chad J; Ittmann, Michael; Ozen, Mustafa

    2016-03-01

    The cancer stem-like cells (CSLCs) are tumorigenic cells promoting initiation, progression, and spread of the tumor. Accumulating evidences suggested the presence of CSLCs in distinct tumors including laryngeal squamous cell carcinoma (LSCC). MicroRNAs have been proposed as significant regulators of carcinogenesis, and several of them have been demonstrated to have direct roles in survival of CSLCs. In this study, we aimed to explore the role of miR-145, which is downregulated in LSCC, on cancer stem cell potency of laryngeal cancer cells. We initially showed the downregulation of miR-145 expression in tumor tissue samples and in CD133-enriched CSLCs. Quantitative reverse-transcription PCR (qRT-PCR) analysis of miR-145-transfected Hep-2 cells demonstrated the inhibitory role of miR-145 on stem cell markers like SOX2, OCT4, KLF4, and ABCG2. We, then, investigated the stem cell features of miR-145-overexpressing Hep-2 cells by sphere formation assay, single-cell cloning assay, and aldehyde dehydrogenase (ALDH) assay, which all demonstrated the inhibition of stem cell potency upon miR-145 overexpression. Further qRT-PCR analysis demonstrated altered expression of epithelial to mesenchymal transition markers in miR-145-overexpressing Hep-2 cells. In conclusion, we demonstrated the regulatory role of miR-145 in stem cell characteristics of Hep-2 cells. Based on these results, we propose that miR-145 might carry crucial roles in LSCC tumorigenesis, prognosis, metastasis, chemoresistance, and recurrence through regulating stem cell properties of tumor cells.

  18. MiR-145 functions as a tumor suppressor via regulating angiopoietin-2 in pancreatic cancer cells.

    Science.gov (United States)

    Wang, Hao; Hang, Cheng; Ou, Xi-Long; Nie, Jin-Shan; Ding, Yi-Tao; Xue, Shi-Gui; Gao, Hua; Zhu, Jian-Xin

    2016-01-01

    Pancreatic cancer is currently one of the leading causes of cancer deaths without any effective therapies. Mir-145 has been found to be tumor-suppressive in various types of cancers. The aim of this study is to investigate the role of miR-145 in pancreatic cancer cells and explore its underlying mechanism. Quantitative real time PCR was used to determine the expression level of miR-145 and angiopoietin-2 (Ang-2) mNRA, and the expression level of Ang-2 protein was measured by western blotting. The anti-cancer activities of miR-145 were tested both in in vitro by using cell invasion and colony formation assay and in vivo by using xenograft assay. The direct action of miR-145 on Ang-2 was predicted by TargetScan and confirmed by luciferase report assay. The vascularization of xenografts were performed by immunohistochemical analysis. The expression level of miR-145 was significantly lower and the expression levels of Ang-2 mRNA and protein was significantly higher in the more aggressive pancreatic cancer cells (MiaPaCa-2 and Panc-1) when compared to that in BxPC3 cells. Overexpression of miR-145 in the BxPC3, MiaPaCa-2 and Panc-1 cells suppressed the cell invasion and colony formation ability, and the expression level of Ang-2 protein in MiaPaCa-2 and Panc-1 cells was also suppressed after pre-miR-145 transfection. Intratumoral delivery of miR-145 inhibited the growth of pancreatic cancer xenografts and angiogenesis in vivo, and also suppressed the expression level of angiopoietin-2 protein. Luciferase report assay showed that Ang-2 is a direct target of miR-145, and down-regulation of angiopoietin-2 by treatment with Ang-2 siRNA in the BxPC3, MiaPaCa-2 and Panc-1 cells suppressed cell invasion and colony formation ability. The reverse transcription PCR results also showed that Tie1 and Tie2 were expressed in BxPC3, MiaPaCa-2 and Panc-1 cells. MiR-145 functions as a tumor suppressor in pancreatic cancer cells by targeting Ang-2 for translation repression and thus

  19. Phosphorylation of inositol 1,4,5-trisphosphate analogues by 3-kinase and dephosphorylation of inositol 1,3,4,5-tetrakisphosphate analogues by 5-phosphatase

    NARCIS (Netherlands)

    Dijken, Peter van; Lammers, Aleida A.; Ozaki, Shoichiro; Potter, Barry V.L.; Erneux, Christophe; Haastert, Peter J.M. van

    1994-01-01

    A series of P-32-labeled D-myo-inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P-4] analogues was enzymically prepared from the corresponding D-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P-3] analogues using recombinant rat brain Ins(1,4,5)P-3 3-kinase and [gamma-P-32]ATP. Ins(1,4,5)P-3 analogues

  20. Role of αArg145 and βArg263 in the active site of penicillin acylase of Escherichia coli

    NARCIS (Netherlands)

    Alkema, Wynand B.L.; Prins, Antoon K.; de Vries, Erik; Janssen, Dick B.

    2002-01-01

    The active site of penicillin acylase of Eschcrichia coli contains two conserved arginine residues, The function of these arainines, alphaArg(145) and betaArg(263), was studied by site-directed mutagenesis and kinetic analysis of the mutant enzymes. The mutants alphaArg(145)-->Leu (alphaArg145Leu),

  1. 40 CFR 270.145 - What are the procedures for public comment on the draft RAP or notice of intent to deny?

    Science.gov (United States)

    2010-07-01

    ... comment on the draft RAP or notice of intent to deny? 270.145 Section 270.145 Protection of Environment... HAZARDOUS WASTE PERMIT PROGRAM Remedial Action Plans (RAPs) Getting A Rap Approved § 270.145 What are the procedures for public comment on the draft RAP or notice of intent to deny? (a) The Director must: (1) Send...

  2. Quercetin induces the apoptosis of human ovarian carcinoma cells by upregulating the expression of microRNA-145

    National Research Council Canada - National Science Library

    ZHOU, JUNBO; GONG, JIAN; DING, CHUN; CHEN, GUIQIN

    2015-01-01

    .... Numerous previous studies have demonstrated that microRNA (miR)-145 is downregulated in ovarian cancer, and that quercetin can inhibit the growth of cancer cells via regulating the expression of miRs...

  3. MicroRNA-145 suppresses ROS-induced Ca{sup 2+} overload of cardiomyocytes by targeting CaMKIIδ

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Min-Ji [Cardiovascular Research Institute, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Jang, Jin-Kyung [College of Pharmacy, Sookmyung Women’s University, 52 HyoChangWon-Gil, Yongsan-ku, Seoul 140-742 (Korea, Republic of); Ham, Onju; Song, Byeong-Wook; Lee, Se-Yeon [Cardiovascular Research Institute, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Lee, Chang Yeon; Park, Jun-Hee [Department of Integrated Omics for Biomedical Sciences, Graduate School, Yonsei University, 50 Yonsei-ro, Seodamun-gu, Seoul 120-759 (Korea, Republic of); Lee, Jiyun; Seo, Hyang-Hee [Cardiovascular Research Institute, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Choi, Eunhyun [Severance Integrative Research Institute for Cerebral and Cardiovascular Disease, Yonsei University Health System, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Jeon, Woo-min [Department of Animal Resource, Sahmyook University, Seoul 139-742 (Korea, Republic of); Hwang, Hye Jin [Cardiovascular Research Institute, Yonsei University College of Medicine, 250 Seongsanno, Seodamun-gu, Seoul 120-752 (Korea, Republic of); Shin, Hyun-Taek [College of Pharmacy, Sookmyung Women’s University, 52 HyoChangWon-Gil, Yongsan-ku, Seoul 140-742 (Korea, Republic of); and others

    2013-06-14

    Highlights: •CaMKIIδ mediates H{sub 2}O{sub 2}-induced Ca{sup 2+} overload in cardiomyocytes. •miR-145 can inhibit Ca{sup 2+} overload. •A luciferase assay confirms that miR-145 functions as a CaMKIIδ-targeting miRNA. •Overexpression of miR-145 regulates CaMKIIδ-related genes and ameliorates apoptosis. -- Abstract: A change in intracellular free calcium (Ca{sup 2+}) is a common signaling mechanism of reperfusion-induced cardiomyocyte death. Calcium/calmodulin dependent protein kinase II (CaMKII) is a critical regulator of Ca{sup 2+} signaling and mediates signaling pathways responsible for functions in the heart including hypertrophy, apoptosis, arrhythmia, and heart disease. MicroRNAs (miRNA) are involved in the regulation of cell response, including survival, proliferation, apoptosis, and development. However, the roles of miRNAs in Ca{sup 2+}-mediated apoptosis of cardiomyocytes are uncertain. Here, we determined the potential role of miRNA in the regulation of CaMKII dependent apoptosis and explored its underlying mechanism. To determine the potential roles of miRNAs in H{sub 2}O{sub 2}-mediated Ca{sup 2+} overload, we selected and tested 6 putative miRNAs that targeted CaMKIIδ, and showed that miR-145 represses CaMKIIδ protein expression and Ca{sup 2+} overload. We confirmed CaMKIIδ as a direct downstream target of miR-145. Furthermore, miR-145 regulates Ca{sup 2+}-related signals and ameliorates apoptosis. This study demonstrates that miR-145 regulates reactive oxygen species (ROS)-induced Ca{sup 2+} overload in cardiomyocytes. Thus, miR-145 affects ROS-mediated gene regulation and cellular injury responses.

  4. Computer-assisted assessment of ultrasound real-time elastography: Initial experience in 145 breast lesions

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Xue; Xiao, Yang [Shenzhen Key Lab for Molecular Imaging, Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen (China); Zeng, Jie [Department of Medical Ultrasonics, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou (China); Qiu, Weibao; Qian, Ming; Wang, Congzhi [Shenzhen Key Lab for Molecular Imaging, Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen (China); Zheng, Rongqin, E-mail: zhengronggin@hotmail.com [Department of Medical Ultrasonics, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou (China); Zheng, Hairong, E-mail: hr.zheng@siat.ac.cn [Shenzhen Key Lab for Molecular Imaging, Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen (China)

    2014-01-15

    Purpose: To develop and evaluate a computer-assisted method of quantifying five-point elasticity scoring system based on ultrasound real-time elastography (RTE), for classifying benign and malignant breast lesions, with pathologic results as the reference standard. Materials and methods: Conventional ultrasonography (US) and RTE images of 145 breast lesions (67 malignant, 78 benign) were performed in this study. Each lesion was automatically contoured on the B-mode image by the level set method and mapped on the RTE image. The relative elasticity value of each pixel was reconstructed and classified into hard or soft by the fuzzy c-means clustering method. According to the hardness degree inside lesion and its surrounding tissue, the elasticity score of the RTE image was computed in an automatic way. Visual assessments of the radiologists were used for comparing the diagnostic performance. Histopathologic examination was used as the reference standard. The Student's t test and receiver operating characteristic (ROC) curve analysis were performed for statistical analysis. Results: Considering score 4 or higher as test positive for malignancy, the diagnostic accuracy, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were 93.8% (136/145), 92.5% (62/67), 94.9% (74/78), 93.9% (62/66), and 93.7% (74/79) for the computer-assisted scheme, and 89.7% (130/145), 85.1% (57/67), 93.6% (73/78), 92.0% (57/62), and 88.0% (73/83) for manual assessment. Area under ROC curve (A{sub z} value) for the proposed method was higher than the A{sub z} value for visual assessment (0.96 vs. 0.93). Conclusion: Computer-assisted quantification of classical five-point scoring system can significantly eliminate the interobserver variability and thereby improve the diagnostic confidence of classifying the breast lesions to avoid unnecessary biopsy.

  5. A new calmodulin-binding motif for inositol 1,4,5-trisphosphate 3-kinase regulation.

    Science.gov (United States)

    Franco-Echevarría, Elsa; Baños-Sanz, Jose I; Monterroso, Begoña; Round, Adam; Sanz-Aparicio, Julia; González, Beatriz

    2014-11-01

    IP3-3K [Ins(1,4,5)P3 3-kinase] is a key enzyme that catalyses the synthesis of Ins(1,3,4,5)P4, using Ins(1,4,5)P3 and ATP as substrates. Both inositides, substrate and product, present crucial roles in the cell. Ins(1,4,5)P3 is a key point in Ca2+ metabolism that promotes Ca2+ release from intracellular stores and together with Ins(1,3,4,5)P4 regulates Ca2+ homoeostasis. In addition, Ins(1,3,4,5)P4 is involved in immune cell development. It has been proved that Ca2+/CaM (calmodulin) regulates the activity of IP3-3K, via direct interaction between both enzymes. Although we have extensive structural knowledge of the kinase domains of the three IP3-3K isoforms, no structural information is available about the interaction between IP3-3K and Ca2+/CaM. In the present paper we describe the crystal structure of the complex between human Ca2+/CaM and the CaM-binding region of human IP3-3K isoform A (residues 158-183) and propose a model for a complex including the kinase domain. The structure obtained allowed us to identify all of the key residues involved in the interaction, which have been evaluated by site-directed mutagenesis, pull-down and fluorescence anisotropy experiments. The results allowed the identification of a new CaM-binding motif, expanding our knowledge about how CaM interacts with its partners.

  6. VizieR Online Data Catalog: 2015 TB145 light curve (Mueller+, 2017)

    Science.gov (United States)

    Mueller, T. G.; Marciniak, A.; Butkiewicz-Bak, M.; Duffard, R.; Oszkiewicz, D.; Kaeufl, H. U.; Szakats, R.; Santana-Ros, T.; Kiss, C.; Santana-Sanz, P.

    2016-10-01

    In this paper we used photometric data gained by the 1.23-m telescope at Calar Alto Observatory (CAHA) in Almeria (Spain), the 1.5-m telescope at Sierra Nevada Observatory (OSN) in Granada (Spain) and the 0.80-m telescope at La Hita Observatory, near Toledo, also in Spain. Additionally we worked with data presented by Warner et al. (2016). objects: ------------------------------------------------------ Name H i e a (mag) (deg) (AU) ------------------------------------------------------ 2015 TB145 19.97 19.697116 0.86036548 2.10240435 ------------------------------------------------------ (1 data file).

  7. Nuclear level densities and gamma-ray strength functions of 145,149,151Nd isotopes

    Science.gov (United States)

    Ay, K. O.; Ozgur, M.; Algin, E.; Guttormsen, M.; Bello Garrote, F. L.; Crespo Campo, L.; Görgen, A.; Hagen, T. W.; Ingeberg, V. W.; Kheswa, B. V.; Klintefjord, M.; Larsen, A. C.; Midtbo, J. E.; Modamio, V.; Renstrom, T.; Rose, S. J.; Sahin, E.; Siem, S.; Tveten, G. M.; Zeiser, F.

    2016-10-01

    The nuclear level densities and gamma-ray strength functions are the key elements for Hauser-Feshbach statistical model calculations to predict reaction cross sections which have many applications including astrophysics. The nuclear level densities and y-ray strength functions have been determined for 145,149,151Nd isotopes below the neutron separation energies using the Oslo method with the 144,148,150Nd(d,p) reactions. The results from the first measurements as well as planned experiments at OCL will be presented.

  8. Computer-aided molecular modeling of a thromboxane receptor antagonist S-145 and its related compounds.

    Science.gov (United States)

    Ezumi, K; Yamakawa, M; Narisada, M

    1990-04-01

    Conformational analyses on thromboxane A2 (TxA2), its receptor agonist, U-46619, and its receptor antagonist, sulotroban, were carried out by molecular mechanics (MMFF) or molecular orbital (MNDO) methods. Two kinds of putative active conformations of TxA2 and the agonist were proposed on the basis of these results by referring to the hairpin conformation hypothesis. From the superposition of stable conformers of sulotroban on those conformers, the molecular structural requirements for potent TxA2 receptor antagonism were elucidated. S-145 in which these requirements are satisfied was a very potent TxA2 antagonist.

  9. Xenotransplanted human prostate carcinoma (DU145) cells develop into carcinomas and cribriform carcinomas: ultrastructural aspects.

    Science.gov (United States)

    Gilloteaux, Jacques; Jamison, James M; Neal, Deborah R; Summers, Jack L; Taper, Henryk S

    2012-10-01

    Androgen-independent, human prostate carcinoma cells (DU145) develop into solid, carcinomatous xenotransplants on the diaphragm of nu/nu mice. Tumors encompass at least two poorly differentiated cell types: a rapidly dividing, eosinophilic cell comprises the main cell population and a few, but large basophilic cells able to invade the peritoneal stroma, the muscular tissue, lymph vessels. Poor cell contacts, intracytoplasmic lumina, and signet cells are noted. Lysosomal activities are reflected by entoses and programmed cell deaths forming cribriform carcinomas. In large tumors, degraded cells may align with others to facilitate formation of blood supply routes. Malignant cells would spread via ascites and through lymphatics.

  10. Exposure-related effects of Pseudomonas fluorescens (Pf-CL145A) on juvenile unionid mussels

    Science.gov (United States)

    Weber, Kerry L.; Luoma, James A.; Mayer, Denise A.; Aloisi, Douglas B.; Eckert, Nathan L.

    2015-01-01

    The exposure-related effects of a commercially prepared spray-dried powder (SDP) or freeze-dried powder (FDP) formulation of Pseudomonas fluorescens (strain CL145A) on the survival of seven species of newly metamorphosed (<72 hours old) freshwater unionid mussels was evaluated. Forty unionid mussels of each species were randomly distributed to test chambers and each species independently exposed for 24 hours to a static dose of either SDP (four species: Lampsilis cardium, Lampsilis siliquoidea, Lampsilis higginsii, andLigumia recta) or FDP (three species: Obovaria olivaria, Actinonaias ligamentina, andMegalonaias nervosa).

  11. Synthesis of inositol phosphate-based competitive antagonists of inositol 1,4,5-trisphosphate receptors

    OpenAIRE

    Konieczny, Vera; Stefanakis, John G.; Sitsanidis, Efstratios; Ioannidou, Natalia-Anastasia T.; Papadopoulos, Nikolaos V.; Fylaktakidou, Konstantina C.; Taylor, Colin W.; Koumbis, Alexandros E.

    2016-01-01

    This is the final version of the article. It first appeared from the Royal Chemistry Society via http://dx.doi.org/10.1039/C5OB02623G Inositol 1,4,5-trisphosphate receptors (IP3R) are intracellular Ca²⁺ channels that are widely expressed in animal cells, where they mediate the release of Ca²⁺ from intracellular stores evoked by extracellular stimuli. A diverse array of synthetic agonists of IP3Rs has defined structure-activity relationships, but existing antagonists have severe limitations...

  12. Non-target trials with Pseudomonas fluorescens strain CL145A, a lethal control agent of dreissenid mussels (Bivalvia: Dreissenidae

    Directory of Open Access Journals (Sweden)

    Daniel P. Molloy

    2013-01-01

    Full Text Available In an effort to develop an efficacious and environmentally safe method for managing zebra mussels (Dreissena polymorpha and quaggamussels (Dreissena rostriformis bugensis, we initiated a research project investigating the potential use of bacteria and their naturalmetabolic products as biocontrol agents. This project resulted in the discovery of an environmental isolate lethal to dreissenid mussels,Pseudomonas fluorescens strain CL145A (Pf-CL145A. In previous published reports we have demonstrated that: 1 Pf-CL145A’s mode ofaction is intoxication (not infection; 2 natural product within ingested bacterial cells lyse digestive tract epithelial cells leading to dreisseniddeath; and 3 high dreissenid kill rates (>90% are achievable following treatment with Pf-CL145A cells, irrespective of whether thebacterial cells are dead or alive. Investigating the environmental safety of Pf-CL145A was also a key element in our research efforts, andherein, we report the results of non-target trials demonstrating Pf-CL145A’s high specificity to dreissenids. These acute toxicity trials weretypically single-dose, short-term (24-72 h exposures to Pf-CL145A cells under aerated conditions at concentrations highly lethal todreissenids (100 or 200 mg/L. These trials produced no evidence of mortality among the ciliate Colpidium colpoda, the cladoceran Daphniamagna, three fish species (Pimephales promelas, Salmo trutta, and Lepomis macrochirus, and seven bivalve species (Mytilus edulis,Pyganodon grandis, Pyganodon cataracta, Lasmigona compressa, Strophitus undulatus, Lampsilis radiata, and Elliptio complanata. Lowmortality (3-27% was recorded in the amphipod Hyalella azteca, but additional trials suggested that most, if not all, of the mortality couldbe attributed to some other unidentified factor (e.g., possibly particle load or a water quality issue rather than Pf-CL145A’s dreissenidkillingnatural product. In terms of potential environmental safety, the results of

  13. Expression of miR-143 and miR-145 and their functional study in gastric carcinoma%miR-143和miR-145在胃癌中的表达及功能研究

    Institute of Scientific and Technical Information of China (English)

    邢晓芳; 李子禹

    2015-01-01

    Objective To investigate the expressions of miR-143 and miR-145 in gastric cancer tissues and their biological functions. Methods A set of gastric cancer metastasis-related miRNAs was screened by comparing the miRNA profiles between the primary gastric cancer and matched liver metastasis. Among these miRNAs, expressions of miR-143 and miR-145 were further validated. The influence on metastasis was analyzed by transwell assay. Results Expressions of miR-143 and miR-145 down-regulated significantly in gastric cancer tissues compared to adjacent normal tissues (miR-143, 0.028 ±0.005 vs. 0.052 ±0.014, P=0.058; miR-145, 0.922 ±0.135 vs. 1.722 ±0.285, P=0.007), and decreased significantly in the metastatic lesion compared with the primary lesion (miR-143, 0.059±0.025 vs. 0.182±0.045, P=0.021; miR-145, 0.164±0.076 vs. 0.594±0.283, P=0.042). Expressions of miR-143 and miR-145 were significantly correlated (r=0.400, P=0.000), and they synergistically inhibited gastric cancer cell migration. Conclusion miR-143 and miR-145 may take part in the progression of gastric cancer metastasis, and they may have synergetic effects.%目的:探讨miR-143和miR-145在胃癌组织中的表达情况,观察其生物学功能。方法通过高通量芯片检测比较11对胃癌原发灶及其配对肝转移灶的miRNA表达谱,采用Real-time PCR方法观察miR-145和miR-143在55例胃癌组织中的表达情况。同时使用Transwell方法观察其对细胞转移能力的影响。结果 miR-143和miR-145在胃癌组织中的表达明显低于癌旁正常组织(miR-143:0.028±0.005比0.052±0.014,P=0.058;miR-145:0.922±0.135比1.772±0.285,P=0.007),在转移灶中的表达明显低于原发灶(miR-143:0.059±0.025比0.182±0.045,P=0.021;miR-145:0.164±0.076比0.594±0.283,P=0.042),相关性分析显示,miR-143和miR-145表达具有显著相关性(r=0.400, P=0.000)。体外实验显

  14. Stimulation of inositol 1,4,5-trisphosphate (IP3 receptor subtypes by analogues of IP3.

    Directory of Open Access Journals (Sweden)

    Huma Saleem

    Full Text Available Most animal cells express mixtures of the three subtypes of inositol 1,4,5-trisphosphate receptor (IP(3R encoded by vertebrate genomes. Activation of each subtype by different agonists has not hitherto been examined in cells expressing defined homogenous populations of IP(3R. Here we measure Ca(2+ release evoked by synthetic analogues of IP(3 using a Ca(2+ indicator within the lumen of the endoplasmic reticulum of permeabilized DT40 cells stably expressing single subtypes of mammalian IP(3R. Phosphorylation of (1,4,5IP(3 to (1,3,4,5IP(4 reduced potency by ~100-fold. Relative to (1,4,5IP(3, the potencies of IP(3 analogues modified at the 1-position (malachite green (1,4,5IP(3, 2-position (2-deoxy(1,4,5IP(3 or 3-position (3-deoxy(1,4,5IP(3, (1,3,4,5IP(4 were similar for each IP(3R subtype. The potency of an analogue, (1,4,6IP(3, in which the orientations of the 2- and 3-hydroxyl groups were inverted, was also reduced similarly for all three IP(3R subtypes. Most analogues of IP(3 interact similarly with the three IP(3R subtypes, but the decrease in potency accompanying removal of the 1-phosphate from (1,4,5IP(3 was least for IP(3R3. Addition of a large chromophore (malachite green to the 1-phosphate of (1,4,5IP(3 only modestly reduced potency suggesting that similar analogues could be used to measure (1,4,5IP(3 binding optically. These data provide the first structure-activity analyses of key IP(3 analogues using homogenous populations of each mammalian IP(3R subtype. They demonstrate broadly similar structure-activity relationships for all mammalian IP(3R subtypes and establish the potential utility of (1,4,5IP(3 analogues with chromophores attached to the 1-position.

  15. Microstructure and dielectric properties of dysprosium-doped barium titanate ceramics Microestrutura e propriedades dielétricas de cerâmicas de titanato de bário dopado com disprósio

    Directory of Open Access Journals (Sweden)

    Y. Pu

    2005-09-01

    Full Text Available The substitution behavior and lattice parameter of barium titanate between solid_solubility with a dopant concentration in the range of 0.25 to 1.5 mol% are studied. The influences of dysprosium-doped fraction on the grain size and dielectric properties of barium titanate ceramic, including dielectric constant and breakdown electric field strength, are investigated via scanning electronic microscopy, X-ray diffraction and electric property tester. The results show that, at a dysprosium concentration of 0.75 mol%, the abnormal grain growth is inhibited and the lattice parameters of grain rise up to the maximum because of the lowest vacancy concentration. In addition, the finegrain and high density of barium titanate ceramic result in its excellent dielectric properties. The relative dielectric constant (25 °C reaches to 4100. The temperature coefficient of the capacitance varies from -10 to 10% within the temperature range of -15 °C -100 °C, and the breakdown electric field strength (alternating current achieves 3.2 kV/mm. These data suggest that our barium titanate could be used in the manufacture of high voltage ceramic capacitors.Foram estudados o comportamento da substituição e o parâmetro de rede de titanato de bário da solubilidade sólida com uma concentração de dopante na faixa 0,25-1,5 mol%. As influências da fração do dopante disprósio no tamanho de grão e nas propriedades dielétricas da cerâmica de titanato de bário, incluindo constante dielétrica e rigidez dielétrica foram investigadas por meio de microscopia eletrônica de varredura, difração de raios X e teste de propriedades elétricas. Os resultados mostram que a uma concentração de disprósio de 0,75 mol% o crescimento anormal de grão é inibido e os parâmetros de rede aumentam até um máximo devido a menor concentração de vacâncias. Além disso, as cerâmicas de grãos pequenos e alta densidade resultam em excelentes propriedades dielétricas. A

  16. Neodymium Dysprosium Modified Starch- coated Magnetic Fluid Preparation of Ferrite%淀粉包覆镝钕改性铁氧体磁性液体的制备

    Institute of Scientific and Technical Information of China (English)

    林穗云; 周育辉

    2011-01-01

    In this paper, in order to obtain the ferrite magnetic fluids of higher saturation magnetic intensity with simple technology, nanometer magnetite ( Fe3O4 ) particles ware prepared by chemical co - precipitation. And to a certain proportion of Dysprosium Neodymium ferrite magnetic fluids on the modification, we selected starch prepara- tion for the relief of water - based coating of rare earth iron oxide magnetic fluid composites. We also investigated the amount of Nd - Dy, the amount of coating agent, reaction temperature, coating temperature on the performance of the products and the effects of particle size, and its preliminary characterization was also performed . Through experiment,we summed up, under n (Fe) : [ n ( Nd3+ ) + n ( Dy3+ ) ] = 30:1 and n ( Fe3 + ) : n ( Fe2 + ) = 1.70 ~ 1. 75, the ratio for use of dysprosium and neodymium is n(Dy3+ ) : n(Nd3+ ) =4:1, 25%NH3 · H2O(A. R. ) as precipitating agent and pH value conditioner; the reacting system temperature was controlled in 35 ℃, and the pH value was adjusted to 9 ~ 11 ; the best dosage of starch as the relief is O. 0050g each 6OraL magnetic fluids, the temperature of surfactant was controlled in 50℃ and the pH value was adjusted to 2 ~ 3. In such system under the conditions of a water - based rare - earth compound Nd Dy Fe Magnetic, fluid magnetic oxygen was higher than or- dinary water- based ferrite.%为制备工艺简单且饱和磁化强度高的磁流体,本文采用化学共沉淀法制得了纳米磁性Fe304粒子.然后以一定比例的镝钕对铁氧体磁流体改性,选择淀粉为包覆剂制备水基稀土复合铁氧磁流体.考察了镝钕的用量、包覆剂的用量、反应温度、包覆温度等因素对产物粒径及性能的影响,并对其进行了初步的性能表征.实验总结出适宜的条件:在n(Fe):[n(Nd3+)+n(Dy3+)]=30:1,n(Fe3+):n(Fe2+)=1.70~1.75

  17. The XMM Cluster Survey: A Massive Galaxy Cluster at z = 1.45

    Energy Technology Data Exchange (ETDEWEB)

    Stanford, S A; Romer, A K; Sabirli, K; Davidson, M; Hilton, M; Viana, P P; Collins, C A; Kay, S T; Liddle, A R; Mann, R G; Miller, C J; Nichol, R C; West, M J; Conselice, C J; Spinrad, H; Stern, D; Bundy, K

    2006-05-24

    We report the discovery of XMMXCS J2215.9-1738, a massive galaxy cluster at z = 1.45, which was found in the XMM Cluster Survey. The cluster candidate was initially identified as an extended X-ray source in archival XMM data. Optical spectroscopy shows that 6 galaxies within a {approx}60 arcsec diameter region lie at z = 1.45 {+-} 0.01. Model fits to the X-ray spectra of the extended emission yield kT = 7.4{sub -1.8}{sup +2.7} keV (90% confidence); if there is an undetected central X-ray point source then kT = 6.5{sub -1.8}{sup +2.6} keV. The bolometric X-ray luminosity is L{sub x} = 4.4{sub -0.6}{sup +0.8} x 10{sup 44} ergs s{sup -1} over a 2 Mpc radial region. The measured T{sub x}, which is the highest for any known cluster at z > 1, suggests that this cluster is relatively massive for such a high redshift. The redshift of XMMXCS J2215.9-1738 is the highest currently known for a spectroscopically-confirmed cluster of galaxies.

  18. Inositol 1,4,5-trisphosphate receptor 1 is essential to spinocerebellar ataxia modeling

    Directory of Open Access Journals (Sweden)

    Sherry-Ann eBrown

    2015-01-01

    Full Text Available A suite of models was developed to study the role of inositol 1,4,5-trisphosphate receptor 1 (IP3R1 in spinocerebellar ataxias (SCAs. Several SCAs are linked to reduced abundance of IP3R1 or to supranormal sensitivity of the receptor to activation by its ligand inositol 1,4,5-trisphosphate (IP3. Detailed multidimensional models have been created to simulate biochemical calcium signaling and membrane electrophysiology in cerebellar Purkinje neurons. In these models, IP3R1-mediated calcium release is allowed to interact with ion channel response on the cell membrane. Experimental findings in mice and clinical observations in humans provide data input for the models. The SCA modeling suite helps interpret experimental results and provides suggestions to guide experiments. The models predict IP3R1 supersensitivity in SCA1 and compensatory mechanisms in SCA1, SCA2, and SCA3. Simulations explain the impact of calcium buffers. Results show that IP3R1-mediated calcium release activates voltage-gated calcium-activated potassium channels in the plasma membrane. The SCA modeling suite unifies observations from experiments in a number of SCAs. The cadre of simulations demonstrates the central role of IP3R1.

  19. Inositol 1,4,5-trisphosphate 3-kinases: functions and regulations

    Institute of Scientific and Technical Information of China (English)

    Hui Jun XIA; Guang YANG

    2005-01-01

    Inositol 1,4,5-trisphosphate 3-kinase (IP3 3-kinase/IP3K) plays an important role in signal transduction in animal cells by phosphorylating inositol 1,4,5-trisphosphate (IP3) to inositol 1,3,4,5-tetrakisphosphate (IP4). Both IP3 and IP4 are critical second messengers which regulate calcium (Ca2+) homeostasis. Mammalian IP3Ks are involved in many biological processes, including brain development, memory, learning and so on. It is widely reported that Ca2+ is a canonical second messenger in higher plants. Therefore, plant IP3K should also play a crucial role in plant development. Recently,we reported the identification of plant IP3K gene (AtIpk2β/AtIP3K) from Arabidopsis thaliana and its characterization.Here, we summarize the molecular cloning, biochemical properties and biological functions of IP3Ks from animal, yeast and plant. This review also discusses potential functions of IP3Ks in signaling crosstalk, inositol phosphate metabolism,gene transcriptional control and so on.

  20. Regulation of autophagy in cardiomyocytes by Ins(1,4,5)P(3) and IP(3)-receptors.

    Science.gov (United States)

    Wong, Albert; Grubb, David R; Cooley, Nicola; Luo, Jieting; Woodcock, Elizabeth A

    2013-01-01

    Autophagy is a process that removes damaged proteins and organelles and is of particular importance in terminally differentiated cells such as cardiomyocytes, where it has primarily a protective role. We investigated the involvement of inositol(1,4,5)trisphosphate (Ins(1,4,5)P(3)) and its receptors in autophagic responses in neonatal rat ventricular myocytes (NRVM). Treatment with the IP(3)-receptor (IP(3)-R) antagonist 2-aminoethoxydiphenyl borate (2-APB) at 5 or 20 μmol/L resulted in an increase in autophagosome content, defined as puncta labeled by antibody to microtubule associated light chain 3 (LC3). 2-APB also increased autophagic flux, indicated by heightened LC3II accumulation, which was further enhanced by bafilomycin (10nmol/L). Expression of Ins(1,4,5)P(3) 5-phosphatase (IP(3)-5-Pase) to deplete Ins(1,4,5)P(3) also increased LC3-labeled puncta and LC3II content, suggesting that Ins(1,4,5)P(3) inhibits autophagy. The IP(3)-R can act as an inhibitory scaffold sequestering the autophagic effector, beclin-1 to its ligand binding domain (LBD). Expression of GFP-IP(3)-R-LBD inhibited autophagic signaling and furthermore, beclin-1 co-immunoprecipitated with the IP(3)-R-LBD. A mutant GFP-IP(3)-R-LBD with reduced ability to bind Ins(1,4,5)P(3) bound beclin-1 and inhibited autophagy similarly to the wild type sequence. These data provide evidence that Ins(1,4,5)P(3) and IP(3)-R act as inhibitors of autophagic responses in cardiomyocytes. By suppressing autophagy, IP(3)-R may contribute to cardiac pathology.

  1. Construction of the prostate cancer cell line DU145 with Raptor-shRNA%稳定沉默Raptor表达的前列腺癌DU145细胞株的构建

    Institute of Scientific and Technical Information of China (English)

    潘腾飞; 陈先国; 梁朝朝

    2013-01-01

    目的 应用RNA干扰技术降低前列腺癌DU145细胞株Raptor基因表达水平,并建立稳定转染细胞株.方法 针对Raptor基因设计合成siRNA,鉴定、测序正确后转染293T细胞观察基因表达情况;构建Raptor-shRNA慢病毒载体;进行Raptor-shRNA慢病毒包装及滴度测定;筛选稳定表达Raptor-shRNA的前列腺癌DU145细胞株;Western blot检测稳定转染细胞中的Raptor表达水平.结果 Raptor-shRNA成功转染前列腺癌DU145细胞后,通过嘌呤霉素筛选,获得了Raptor基因沉默的DU145细胞株,Western blot检测证实该细胞株的Raptor表达水平明显低于对照组(P<0.01).结论 成功构建稳定沉默Raptor表达的前列腺癌DU145细胞株,为后续研究奠定了基础.

  2. Ins(1,4,5)P3 receptor-mediated Ca2+ signaling and autophagy induction are interrelated.

    Science.gov (United States)

    Decuypere, Jean-Paul; Welkenhuyzen, Kirsten; Luyten, Tomas; Ponsaerts, Raf; Dewaele, Michael; Molgó, Jordi; Agostinis, Patrizia; Missiaen, Ludwig; De Smedt, Humbert; Parys, Jan B; Bultynck, Geert

    2011-12-01

    The role of intracellular Ca2+ signaling in starvation-induced autophagy remains unclear. Here, we examined Ca2+ dynamics during starvation-induced autophagy and the underlying molecular mechanisms. Tightly correlating with autophagy stimulation, we observed a remodeling of the Ca2+ signalosome. First, short periods of starvation (1 to 3 h) caused a prominent increase of the ER Ca2+-store content and enhanced agonist-induced Ca2+ release. The mechanism involved the upregulation of intralumenal ER Ca2+-binding proteins, calreticulin and Grp78/BiP, which increased the ER Ca2+-buffering capacity and reduced the ER Ca2+ leak. Second, starvation led to Ins(1,4,5)P3R sensitization. Immunoprecipitation experiments showed that during starvation Beclin 1, released from Bcl-2, first bound with increasing efficiency to Ins(1,4,5)P3Rs; after reaching a maximal binding after 3 h, binding, however, decreased again. The interaction site of Beclin 1 was determined to be present in the N-terminal Ins(1,4,5)P3-binding domain of the Ins(1,4,5)P3R. The starvation-induced Ins(1,4,5)P3R sensitization was abolished in cells treated with BECN1 siRNA, but not with ATG5 siRNA, pointing toward an essential role of Beclin 1 in this process. Moreover, recombinant Beclin 1 sensitized Ins(1,4,5)P3Rs in 45Ca2+-flux assays, indicating a direct regulation of Ins(1,4,5)P3R activity by Beclin 1. Finally, we found that Ins(1,4,5)P3R-mediated Ca2+ signaling was critical for starvation-induced autophagy stimulation, since the Ca2+ chelator BAPTA-AM as well as the Ins(1,4,5)P3R inhibitor xestospongin B abolished the increase in LC3 lipidation and GFP-LC3-puncta formation. Hence, our results indicate a tight and essential interrelation between intracellular Ca2+ signaling and autophagy stimulation as a proximal event in response to starvation.

  3. Modulation of KCNQ1 current by atrial fibrillation-associated KCNE4 (145E/D) gene polymorphism

    Institute of Scientific and Technical Information of China (English)

    MA Ke-juan; LI Ning; TENG Si-yong; ZHANG Yin-hui; SUN Qi; GU Dong-feng; PU Jie-lin

    2007-01-01

    Background Atrial fibrillation is a common arrhythmia with multi-factorial pathogenesis. Recently, a single nucleotide polymorphism (G/T) at position 1057 in the KCNE4 gene, resulting in a glutamic acid (Glu, E)/aspartic acid (Asp, D) substitution at position 145 of the KCNE4 peptide, was found in our laboratory to be associated with idiopathic atrial fibrillation (atrial fibrillation more frequent with KCNE4 145D). However, the functional effect of the KCNE4 145E/Dpolymorphism is still unknown.Methods We constructed KCNE4 (145E/D) expression plasmids and transiently co-transfected them with the KCNQ1 gene into Chinese hamster ovary-K1 cells and performed whole-cell patch-clamping recording to identify the possible functional consequences of the single nucleotide polymorphism. Quantitative data were analyzed by Student's t test. Probability values less than 0.05 were considered statistically significant. Results A slowly activating, non-inactivating voltage-dependent current ((24.0±2.9) pA/pF, at +60 mV)) could be recorded in the cells transfected with KCNQ1 alone. Co-expression of wild type KCNE4 inhibited the KCNQ1 current ((7.3±1.1) pA/pF)). By contrast, co-expression of KCNE4 (145D) augment the KCNQ1 current ((42.9±3.7) pA/pF)). The V1/2 of activation for the KCNQ1/KCNE4 (145D) current was shifted significantly towards the depolarizing potential compared to that for the KCNQ1 current ((-2.3±0.2) mv vs (-13.0±1.5) mv, P < 0.01)) without changing the slope factorK. Furthermore, KCNE4 (145D) also affected the activation and deactivation kinetics of KCNQ1 channels. Conclusion We provide experimental evidence that the KCNE4 (145E/D) polymorphism exerts the effect of "gain of function" on the KCNQ1 channel. It may underlie the genetic mechanism of atrial fibrillation. Further studies on the functional association between Iks and KCNE4 (145D) polymorphism in cardiac myocytes are suggested.

  4. Phase-variable expression of the 145-kDa surface protein of Brazilian purpuric fever case-clone strains of Haemophilus influenzae biogroup aegyptius.

    Science.gov (United States)

    Rubin, L G

    1995-03-01

    Clonally related strains of Haemophilus influenzae biogroup aegyptius have recently been associated with Brazilian purpuric fever (BPF). Antibodies to a 145-kDa minor outer membrane protein (P145) are bactericidal and protect against experimental bacteremia. To determine if P145 is conserved among case-clone strains, case-clone strains were screened for P145 expression. Assays of a large number of colonies of each strain using colony immunoblot revealed colonies reactive with anti-P145 sera in all 17 case-clone strains. P145 was expressed at a low frequency (0.08%-2.2% of colonies) in 14 strains and at a high frequency (> 98%) in 3 strains. Expression of P145 by reactive colonies was confirmed by SDS-PAGE. Also, anti-P145-nonreactive variant colonies of P145-expressing strains were detected in 0.4%-1.5% of colonies. These findings indicate P145 is conserved among BPF case-clone strains and is subject to phase-variable expression.

  5. Linking structure to function: Recent lessons from inositol 1,4,5-trisphosphate receptor mutagenesis.

    Science.gov (United States)

    Yule, David I; Betzenhauser, Matthew J; Joseph, Suresh K

    2010-06-01

    Great insight has been gained into the structure and function of the inositol 1,4,5 trisphosphate receptor (InsP(3)R) by studies employing mutagenesis of the cDNA encoding the receptor. Notably, early studies using this approach defined the key constituents required for InsP(3) binding in the N-terminus and the membrane spanning regions in the C-terminal domain responsible for channel formation, targeting and function. In this article we evaluate recent studies which have used a similar approach to investigate key residues underlying the in vivo modulation by select regulatory factors. In addition, we review studies defining the structural requirements in the channel domain which comprise the conduction pathway and are suggested to be involved in the gating of the channel.

  6. Differential requirement for MEK Partner 1 in DU145 prostate cancer cell migration

    Directory of Open Access Journals (Sweden)

    Bailey Evangeline M

    2009-11-01

    Full Text Available Abstract ERK signaling regulates focal adhesion disassembly during cell movement, and increased ERK signaling frequently contributes to enhanced motility of human tumor cells. We previously found that the ERK scaffold MEK Partner 1 (MP1 is required for focal adhesion disassembly in fibroblasts. Here we test the hypothesis that MP1-dependent ERK signaling regulates motility of DU145 prostate cancer cells. We find that MP1 is required for motility on fibronectin, but not for motility stimulated by serum or EGF. Surprisingly, MP1 appears not to function through its known binding partners MEK1 or PAK1, suggesting the existence of a novel pathway by which MP1 can regulate motility on fibronectin. MP1 may function by regulating the stability or expression of paxillin, a key regulator of motility.

  7. Redetermination of the borax structure from laboratory X-ray data at 145 K

    Directory of Open Access Journals (Sweden)

    Caleb Higham

    2008-05-01

    Full Text Available The title compound, sodium tetraborate decahydrate (mineral name: borax, Na2[B4O5(OH4]·8H2O, has been studied previously using X-ray [Morimoto (1956. Miner. J. 2, 1–18] and neutron [Levy & Lisensky (1978. Acta Cryst. B34, 3502–3510] diffraction data. The structure contains tetraborate anions [B4O5(OH4]2− with twofold rotation symmetry, which form hydrogen-bonded chains, and [Na(H2O6] octahedra that form zigzag chains [Na(H2O4/2(H2O2/1]. The O—H bond distances obtained from the present redetermination at 145 K are shorter than those in the neutron study by an average of 0.127 (19 Å.

  8. Development of a momentum determined electron beam in the 1-45 GeV range

    Energy Technology Data Exchange (ETDEWEB)

    Batarin, V.A.; Butler, J.; Derevschikov, A.A.; Fomin, Yu.V.; Frolov, V.; Grishin, V.N.; Kachanov, V.A.; Khodyrev, V.Y.; Konstantinov, A.S.; Kravtsov, V.I.; Kubota, Y.; Leontiev, V.M.; Maisheev, V.A.; Matulenko, Ya.A.; Meschanin, A.P.; Melnick, Y.M.; Minaev, N.G.; Mochalov, V.V.; Morozov, D.A.; Nogach, L.V.; Semenov, P.A. E-mail: semenov@mx.ihep.su; Shestermanov, K.E.; Soloviev, L.F.; Solovianov, V.L.; Stone, S.; Ukhanov, M.N.; Uzunian, A.V.; Vasiliev, A.N.; Yakutin, A.E.; Yarba, J

    2003-09-11

    A beam line for electrons with energies in the range of 1-45 GeV, low contamination of hadrons and muons and high intensity up to 10{sup 6} per accelerator spill at 27 GeV was setup at U70 accelerator in Protvino, Russia. A beam tagging system based on drift chambers with 160 {mu}m resolution was able to measure relative electron beam momentum precisely. The resolution {sigma}{sub p}/p was 0.13% at 45 GeV where multiple scattering is negligible. This test beam setup provided a possibility to study properties of lead tungstate crystals (PbWO{sub 4}) for the BTeV experiment at Fermilab.

  9. Regulation of the inositol 1,4,5-trisphosphate receptor by tyrosine phosphorylation.

    Science.gov (United States)

    Jayaraman, T; Ondrias, K; Ondriasová, E; Marks, A R

    1996-06-07

    Tyrosine kinases indirectly raise intracellular calcium concentration ([Ca2+]i) by activating phospholipases that generate inositol 1,4,5-trisphosphate (IP3). IP3 activates the IP3 receptor (IP3R), an intracellular calcium release channel on the endoplasmic reticulum. T cell receptor stimulation triggered a physical association between the nonreceptor protein tyrosine kinase Fyn and the IP3R, which induced tyrosine phosphorylation of the IP3R. Fyn activated an IP3-gated calcium channel in vitro, and tyrosine phosphorylation of the IP3R during T cell activation was reduced in thymocytes from fyn-/- mice. Thus, activation of the IP3R by tyrosine phosphorylation may play a role in regulating [Ca2+]i.

  10. 菲律宾蛤仔酶解多肽抗前列腺癌DU-145细胞的活性研究%Study on Antiproliferative Activity of Ruditapes philippinarum Hydrolysate on Human Prostate Cancer DU-145 Cell

    Institute of Scientific and Technical Information of China (English)

    李连军; 徐律; 杨最素; 丁国芳; 孙瑜

    2013-01-01

    [Objective] To study Philippines clam enzymolysis peptide on prostate cancer DU-145 cells proliferation activity in vitro.[Method] Digestive enzyme was used to enzymolysis the Philippine clam guts,3 kD molecular weight cut-off membranes were used to fractionate the Ruditapes philippinarum hydrolysate.The anti-proliferation activity and the early apoptosis of Ruditapes philippinarum peptides in vitro were detected by MTT,inverted microscope and AO/EB double staining respectively.[Result] Determined by MTT method,different concentration of peptides to DU-145 cells had obvious growth inhibition.Cell apoptosis morphological feature was observed under AO/EB double staining and inverted microscope.[Conclusion] Ruditapes philippinarum under 3 kD enzyme solution of molecular weight with inhibition of DU-145 cells growth,induce prostate cancer cells apoptosis,and a concentration and time dependence.%[目的]探讨菲律宾蛤仔酶解多肽抗人前列腺癌DU-145细胞增殖的活性.[方法]对菲律宾蛤仔内脏进行酶解,经超滤截取3 kD以下的多肽,应用MTT法、倒置显微镜观察和AO/EB染色等方法检测其体外抗DU-145细胞的活性.[结果]MTT法检测结果表明不同浓度的多肽对DU-145细胞有明显的生长抑制作用.通过倒置显微镜观察和AO/EB荧光双染观察到DU-145细胞出现细胞凋亡的形态学特征.[结论]菲律宾蛤仔3 kD以下的多肽具有抑制DU-145细胞生长,呈浓度和时间依赖性,并诱导细胞凋亡作用.

  11. Inhibition of highly pathogenic PRRSV replication in MARC-145 cells by artificial microRNAs

    Directory of Open Access Journals (Sweden)

    Wang Liangliang

    2011-11-01

    Full Text Available Abstract Background Highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS has caused large economic losses in swine industry in recent years. However, current antiviral strategy could not effectively prevent and control this disease. In this research, five artificial microRNAs (amiRNAs respectively targeted towards ORF5 (amirGP5-243, -370 and ORF6 (amirM-82, -217,-263 were designed and incorporated into a miRNA-based vector that mimics the backbone of murine miR-155 and permits high expression of amiRNAs in a GFP fused form mediated by RNA Pol II promoter CMV. Results It was found that amirGP5-370 could effectively inhibit H-PRRSV replication. The amirM-263-M-263, which was a dual pre-amiRNA expression cassette where two amirM-263s were chained, showed stronger virus inhibitory effects than single amirM-263. H-PRRSV replication was inhibited up to 120 hours in the MARC-145 cells which were stably transduced by recombinant lentiviruses (Lenti-amirGP5-370, -amirM-263-M-263. Additionally, efficacious dose of amirGP5-370 and amirM-263 expression did not trigger the innate interferon response. Conclusions Our study is the first attempt to suppress H-PRRSV replication in MARC-145 cells through vector-based and lentiviral mediated amiRNAs targeting GP5 or M proteins coding sequences of PRRSV, which indicated that artificial microRNAs and recombinant lentiviruses might be applied to be a new potent anti-PRRSV strategy.

  12. Effect of a triaxial nuclear shape on proton tunneling : the decay and structure of {sup 145}Tm.

    Energy Technology Data Exchange (ETDEWEB)

    Seweryniak, D.; Blank, B.; Carpenter, M. P.; Davids, C. N.; Davinson, T.; Freeman, S. J.; Hammond, N.; Hoteling, N.; Janssens, R. V. F.; Khoo, T. L.; Liu, Z.; Mukherjee, G.; Robinson, A.; Scholey, C.; Sinha, S.; Shergur, J.; Starosta, K.; Walters, W. B.; Woehr, A.; Woods, P. J.; Physics; Centre d' Etudes Nucleaires de Bordeaux-Gradignan; Univ. of Edinburgh; Univ. of Manchester; Univ. of Maryland; Univ. of Jyvaskyla; Michigan State Univ.; Univ. of Notre Dame

    2007-08-24

    Gamma rays deexciting states in the proton emitter {sup 145}Tm were observed using the recoil-decay tagging method. The {sup 145}Tm ground-state rotational band was found to exhibit the properties expected for an h{sub 11/2} proton decoupled band. In addition, coincidences between protons feeding the 2{sup +} state in {sup 144}Er and the 2{sup +} {yields} 0{sup +} {gamma}-ray transition were detected the first measurement of this kind, leading to a more precise value for the 2+ excitation energy of 329(1) keV. Calculations with the particle-rotor model and the core quasiparticle coupling model indicate that the properties of the {pi}h{sub 11/2} band and the proton-decay rates in {sup 145}Tm are consistent with the presence of triaxiality with an asymmetry parameter {gamma} {approx} 20 degrees.

  13. Quercetin induces the apoptosis of human ovarian carcinoma cells by upregulating the expression of microRNA-145.

    Science.gov (United States)

    Zhou, Junbo; Gong, Jian; Ding, Chun; Chen, Guiqin

    2015-08-01

    Ovarian cancer is one of the most malignant types of cancer of the female human reproductive track, posing a severe threat to the health of the female population. Numerous previous studies have demonstrated that microRNA (miR)-145 is downregulated in ovarian cancer, and that quercetin can inhibit the growth of cancer cells via regulating the expression of miRs. Therefore, the present study investigated the effect of quercetin on the expression of miR-145 in SKOV-3 and A2780 human ovarian cancer cell lines. The results revealed that the expression levels of cleaved caspase-3 in the SKOV-3 and A2780 cells were significantly increased following treatment to induce overexpression of miR-145 compared with treatment with quercetin alone (Pquercetin induced the apoptosis of human ovarian carcinoma cells through activation of the extrinsic death receptor mediated and intrinsic mitochondrial apoptotic pathways.

  14. MicroRNAs regulate human adipocyte lipolysis: effects of miR-145 are linked to TNF-α.

    Directory of Open Access Journals (Sweden)

    Silvia Lorente-Cebrián

    Full Text Available MicroRNAs (miRNAs are small non-coding RNAs that regulate gene expression and have multiple effects in various tissues including adipose inflammation, a condition characterized by increased local release of the pro-lipolytic cytokine tumor necrosis factor-alpha (TNF-α. Whether miRNAs regulate adipocyte lipolysis is unknown. We set out to determine whether miRNAs affect adipocyte lipolysis in human fat cells. To this end, eleven miRNAs known to be present in human adipose tissue were over-expressed in human in vitro differentiated adipocytes followed by assessments of TNF-α and glycerol levels in conditioned media after 48 h. Three miRNAs (miR-145, -26a and let-7d modulated both parameters in parallel. However, while miR-26a and let-7d decreased, miR-145 increased both glycerol release and TNF-α secretion. Further studies were focused therefore on miR-145 since this was the only stimulator of lipolysis and TNF-α secretion. Time-course analysis demonstrated that miR-145 over-expression up-regulated TNF-α expression/secretion followed by increased glycerol release. Increase in TNF-α production by miR-145 was mediated via activation of p65, a member of the NF-κB complex. In addition, miR-145 down-regulated the expression of the protease ADAM17, resulting in an increased fraction of membrane bound TNF-α, which is the more biologically active form of TNF-α. MiR-145 overexpression also increased the phosphorylation of activating serine residues in hormone sensitive lipase and decreased the mRNA expression of phosphodiesterase 3B, effects which are also observed upon TNF-α treatment in human adipocytes. We conclude that miR-145 regulates adipocyte lipolysis via multiple mechanisms involving increased production and processing of TNF-α in fat cells.

  15. Role of the 145-kilodalton surface protein in virulence of the Brazilian purpuric fever clone of Haemophilus influenzae biogroup aegyptius for infant rats.

    Science.gov (United States)

    Rubin, L G

    1995-09-01

    Brazilian purpuric fever (BPF) is a fulminant infection associated with bacteremia with clonally related strains of Haemophilus influenzae biogroup aegyptius. Case-associated clone strains are more virulent for infant rats than are non-BPF case-associated H. influenzae biogroup aegyptius isolates. I sought to determine the possible role of P145, a 145-kDa surface protein of BPF case H. influenzae biogroup aegyptius clone isolates, in virulence. First, I compared the virulence of two case-associated clone isolates from the blood of children with BPF from Serrana, Brazil, which differed in P145 expression but were identical in all other phenotypic and genotypic characteristics studied. Twenty-four hours after intraperitoneal inoculation of infant rats, there was a significantly higher incidence (51 versus 26%; P = 0.035) and magnitude (2.9 +/- 5.8 versus 0.7 +/- 2.0 CFU/0.01 ml; P = 0.024) of bacteremia in rats inoculated with the P145-expressing strain. I next compared the virulence of a P145-expressing case-associated clone strain with two P145-nonexpressing phase variants of this strain. One variant exhibited a lower mean magnitude of bacteremia and one displayed a similar magnitude of bacteremia compared with that displayed the P145-expressing parental strain. P145-expressing revertants of the P145-nonexpressing strains exhibited the same virulence as the P145-negative variants from which they were derived. Colonies grown from blood cultures maintained the P145 phenotype of the inoculated strain. These results suggest that P145 expression does not contribute to the virulence of the BPF case clone strain for infant rats following intraperitoneal inoculation.

  16. Study on Current Tests of 145 kV Circuit Breakers for Capacitor Bank Current Switching%145kV断路器开合电容器组电流试验研究

    Institute of Scientific and Technical Information of China (English)

    黄实; 崔博源; 姚斯立; 李志兵; 刘浩军

    2012-01-01

    The standard selection, test method, parameters of test circuit, and test requirement for the current tests of 145 kV circuit breakers for capacitor bank switching are researched, and the test situation is introduced. The performance of 145 kV circuit breakers for capacitor bank switching is examined by using the conclusion of this research to ensure their security and reliability.%笔者研究了145 kV断路器开合电容器组电流试验的标准选用、试验方法、试验回路参数、试验要求等,并介绍了试验情况.研究成果的应用使得145 kV投切电容器组断路器的开合电容器组性能得到了型式试验考核,为工程的安全可靠运行提供了保障.

  17. 表面修饰对镝铁氧体纳米磁粒子的合成及其磁性能的影响%Effect of Surface Modification on Formation and Magnetic Property of Dysprosium Ferrite Nanomagnetic Particles

    Institute of Scientific and Technical Information of China (English)

    陈静; 张茂润; 邓真娟

    2011-01-01

    利用湿化学法制备镝铁氧体纳米磁粒子时,用适量的阴离子表面活性剂进行表面修饰,能够有效地控制磁粒子的粒径,同时避免干燥时产生硬团聚.文章介绍了用月桂酸、月桂酸钠、正十二烷基硫酸钠对磁粒子进行表面修饰的研究结果,探讨了三者及其用量对磁粒子的形成及磁性能的影响.借助X射线衍射仪(XRD)、透射电镜(TEM)、振动样品磁强计(VSM)、红外光谱仪(IR)对产物的性能进行了表征.结果表明,用月桂酸修饰后的产物具有Fe3O4磁粒子的晶型结构且结晶度高、磁性能优异、平均粒径约16nm;用月桂酸钠、正十二烷基硫酸钠修饰后的产物不具有Fe3O4磁粒子的晶型结构,结晶度低且磁性能差.%When wet chemical method are use to prepare dysprosium-doped ferrite magnetic nanoparticles, right a-mount of anionic surfactant were doped as surface modification, which can effectively control the size of magnetic particles, and avoid producing hard reunion when dry out. This paper introduces the research results of magnetic particle surface modification by using lauric acid, laurel acid sodium and lauryl sodium sulfate, discusses the effect of lauric acid, laurel acid sodium and lauryl sodium sulfate and their dosages on the formation of magnetic and its magnetic property. The properties of the product can be characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), infrared spectrometer (IR). The results showed that the product modified with lauric acid have a crystalline structure of Fe3O4 magnetic particles and high crystallin-ity, excellent magnetic properties. The average particle size is about 16 nm; the product modified with laurel acid sodium and lauryl sodium sulfate does not have the crystalline structure of Fe3O4 magnetic particles, with low crystal-linity and low magnetic property.

  18. Flood forecast sensitivity to temperature using ECMWF ensembles for 145 catchments in Norway

    Science.gov (United States)

    Jahr Hegdahl, Trine; Engeland, Kolbjørn; Grønbech, Bård Johan; Steinsland, Ingelin; Merete Tallaksen, Lena

    2017-04-01

    The Norwegian flood forecasting service is based on a flood-forecasting model run on 145 basins. The basins are located all across Norway and differ in both size and hydrological regime. Current flood forecasting system is based on deterministic meteorological forecasts, and uses an auto-regressive procedure to achieve probabilistic forecasts. An alternative approach is to use meteorological and hydrological ensemble forecasts to quantify the uncertainty in forecasted streamflow. The aim of our study is to establish and assess the performance of both meteorological and hydrological ensembles for 145 catchments in Norway, which differ in size, elevation and hydrological regime. We identify regional differences and improvements in performance for preprocessed meteorological forecasts. A separate study further investigates the sensitivity to forecasted temperature for specific snowmelt induced floods. In Norway, snowmelt and combined rain and snowmelt floods are frequent. Hence, temperature is important for correct calculations of snowmelt. Temperature and precipitation ensembles are derived from ECMWF covering a period of nearly three years (01.03.2013 to 31.12.2015). To improve the spread and reduce bias we used standard methods provided by the Norwegian Meteorological Institute. Precipitation is corrected applying a zero-adjusted gamma distribution method (correcting the spread), and temperature is bias corrected using a quantile-quantile mapping (using Hirlam (RCM) 5 km temperature grid as a reference). Observed temperature and precipitation data are station data for all of Norway, interpolated to a 1×1 km2 grid (SeNorge.no). Streamflow observations are available from the NVE database. The hydrological model is the flood-forecasting operational HBV model, run with daily catchment average values. The results show that the methods applied to meteorological ensemble data reduce the cold bias present in the ECMWF temperature ensembles. Catchments on the western coast

  19. Emerging roles of circRNA_001569 targeting miR-145 in the proliferation and invasion of colorectal cancer

    Science.gov (United States)

    Lan, Xiaoliang; Lu, Guifeng; Lin, Yuan; Yang, Shaoshan; Zeng, Zhicheng; Liao, Wenting; Ding, Yan-Qing; Liang, Li

    2016-01-01

    Circular RNAs (circRNAs), a large class of RNAs, have recently shown huge capabilities as gene regulators in mammals. Some of them bind with microRNAs (miRNAs) and act as natural miRNA sponges to inhibit related miRNAs’ activities. Here we showed that hsa_circ_001569 acted as a positive regulator in cell proliferation and invasion of colorectal cancer (CRC). Moreover, hsa_circ_001569 was identified as a sponge of miR-145 and up-regulated miR-145 functional targets E2F5, BAG4 and FMNL2. In CRC tissues, circ_001569 negatively correlated with miR-145, and miR-145 correlated negatively with E2F5, BAG4 and FMNL2 expressions. Our study reveals a novel regulatory mechanism of circ_001569 in cell proliferation and invasion in CRC, provides a comprehensive landscape of circ_001569 that will facilitate further biomarker discoveries in the progression of CRC. PMID:27058418

  20. 42 CFR 435.145 - Children for whom adoption assistance or foster care maintenance payments are made.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Children for whom adoption assistance or foster... Coverage of the Categorically Needy Mandatory Coverage of Adoption Assistance and Foster Care Children § 435.145 Children for whom adoption assistance or foster care maintenance payments are made. The agency...

  1. Cytotoxic evaluation of different fractions of Salvia chorassanica Bunge on MCF-7 and DU 145 cell lines.

    Science.gov (United States)

    Golshan, Alireza; Amini, Elaheh; Emami, Seyed Ahmad; Asili, Javad; Jalali, Zahra; Sabouri-Rad, Sarvenaz; Sanjar-Mousavi, Naghmeh; Tayarani-Najaran, Zahra

    2016-01-01

    Because of antimicrobial, antioxidant, and anticancer potential, Salvia chorassanica Bunge (Lamiaceae) has been considered as a popular herb in Iranian traditional medicine. Previous studies have shown remarkable cytotoxic properties of the methanol, n-hexane and dichloromethane extract of S. chorassanica on human cervical cancer cells. To seek the therapeutic potentials of S. chorassanica, this study was undertaken to evaluate the cytotoxic activities of various extracts of this plant on human breast MCF-7 and prostate cancer DU 145 cells. The DU 145 cells were exposed to different concentrations of plant extracts (1-200 μg/ml). Cytotoxic activities were examined using alamarBlue(®) assay and apoptosis was assessed by acridine orange/propodium iodide double staining and evaluation of DNA fragmentation by flow cytometry. Our findings indicated that n-hexane and dichloromethane extracts had more cytotoxic activities against DU 145 and MCF-7 cell lines compared with other extracts (P<0.05). The acridine orange/propodium iodide staining showed apoptogenic properties of n-hexane and dichloromethane extracts which was consequently confirmed by flow cytometric histogram that exhibited an increase in sub-G1 peak in treated cells as compared with untreated cancer cell lines. Taken together, these observations demonstrated cytotoxic effects of S. chorassanica extracts on MCF-7 and DU 145 cell lines which is most likely exerted via apoptosis cell death. Therefore, further investigations on S. chorassanica extracts as potential chemotherapeutic agents are warranted.

  2. 14 CFR 91.145 - Management of aircraft operations in the vicinity of aerial demonstrations and major sporting...

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Management of aircraft operations in the... OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Flight Rules General § 91.145 Management of aircraft... Balloon Fiesta; (10) Sandia Classic Hang Gliding Competition; (11) Indianapolis 500 Mile Race; (12) Any...

  3. c-Met inhibitor SU11274 enhances the response of the prostate cancer cell line DU145 to ionizing radiation

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Hongliang; Li, Xiaoying; Sun, Shaoqian [Department of Radiation Oncology, Peking University First Hospital, Peking University, Beijing (China); Gao, Xianshu, E-mail: xsgao777@hotmail.com [Department of Radiation Oncology, Peking University First Hospital, Peking University, Beijing (China); Zhou, Demin, E-mail: deminzhou@bjmu.edu.cn [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing (China)

    2012-10-26

    Highlights: Black-Right-Pointing-Pointer c-Met inhibition could significantly enhance the radiosensitivity of DU145 cells. Black-Right-Pointing-Pointer The mechanisms of the radiosensitization effect of c-Met inhibition on DU145 cells were also presented in this paper. Black-Right-Pointing-Pointer This is the first study demonstrating the effectiveness of c-Met inhibition on treating HRPC cells with radiotherapy. -- Abstract: Hormone-refractory prostate cancer shows substantial resistance to most conventional therapies including radiotherapy, constitutes a key impediment to curing patients with the disease. c-Met overexpression plays a key role in prostate cancer tumorigenesis and disease progression. Here, we demonstrate that c-Met inhibition by SU11274 could significantly suppress cell survival and proliferation as well as enhance the radiosensitivity of DU145 cells. The underlying mechanisms of the effects of SU11274 on DU145 cells may include the inhibition of c-Met signaling, depolarization of the mitochondrial membrane potential, impairment of DNA repair function, abrogation of cell cycle arrest, and enhancement of cell death. Our study is the first to show the effectiveness of combining c-Met inhibition with ionizing radiation to cure hormone-refractory prostate cancer.

  4. Cytotoxic evaluation of different fractions of Salvia chorassanica Bunge on MCF-7 and DU 145 cell lines

    Directory of Open Access Journals (Sweden)

    Alireza Golshan

    2016-01-01

    Full Text Available Because of antimicrobial, antioxidant, and anticancer potential, Salvia chorassanica Bunge (Lamiaceae has been considered as a popular herb in Iranian traditional medicine. Previous studies have shown remarkable cytotoxic properties of the methanol, n-hexane and dichloromethane extract of S. chorassanica on human cervical cancer cells. To seek the therapeutic potentials of S. chorassanica, this study was undertaken to evaluate the cytotoxic activities of various extracts of this plant on human breast MCF-7 and prostate cancer DU 145 cells. The DU 145 cells were exposed to different concentrations of plant extracts (1-200 μg/ml. Cytotoxic activities were examined using alamarBlue ® assay and apoptosis was assessed by acridine orange/propodium iodide double staining and evaluation of DNA fragmentation by flow cytometry. Our findings indicated that n-hexane and dichloromethane extracts had more cytotoxic activities against DU 145 and MCF-7 cell lines compared with other extracts (P<0.05. The acridine orange/propodium iodide staining showed apoptogenic properties of n-hexane and dichloromethane extracts which was consequently confirmed by flow cytometric histogram that exhibited an increase in sub-G1 peak in treated cells as compared with untreated cancer cell lines. Taken together, these observations demonstrated cytotoxic effects of S. chorassanica extracts on MCF-7 and DU 145 cell lines which is most likely exerted via apoptosis cell death. Therefore, further investigations on S. chorassanica extracts as potential chemotherapeutic agents are warranted.

  5. A Novel, Phospholipase C-independent Pathway of Inositol 1,4,5-Trisphosphate Formation in Dictyostelium and Rat Liver

    NARCIS (Netherlands)

    Dijken, Peter van; Haas, Jan-Roelof de; Craxton, Andrew; Erneux, Christophe; Shears, Stephen B.; Haastert, Peter J.M. van

    1995-01-01

    In an earlier study a mutant Dictyostelium cell-line (plc(-)) was constructed in which all phospholipase C activity was disrupted and nonfunctional, yet these cells had nearly normal Ins(1,4,5)P-3 levels (Drayer, A. L., Van Der Kaay, J., Mayr, G. W, Van Haastert, P. J. M. (1990) EMBO J. 13, 1601-160

  6. Role of IGF-1/IGF-1R in regulation of invasion in DU145 prostate cancer cells

    Science.gov (United States)

    Saikali, Zeina; Setya, Hemani; Singh, Gurmit; Persad, Sujata

    2008-01-01

    Background Prostate cancer progression to androgen independence is the primary cause of mortality by this tumor type. The IGF-1/IGF-1R axis is well known to contribute to prostate cancer initiation, but its contribution to invasiveness and the downstream signalling mechanisms that are involved are unclear at present. Results We examined the invasive response of androgen independent DU145 prostate carcinoma cells to IGF-1 stimulation using Matrigel assays. We then examined the signaling mechanisms and protease activities that are associated with this response. IGF-1 significantly increased the invasive capacity of DU145 cells in vitro, and this increase was inhibited by blocking IGF-1R. We further demonstrated that specific inhibitors of the MAPK and PI3-K pathways decrease IGF-1-mediated invasion. To determine potential molecular mechanisms for this change in invasive capacity, we examined changes in expression and activity of matrix metalloproteinases. We observed that IGF-1 increases the enzymatic activity of MMP-2 and MMP-9 in DU145 cells. These changes in activity are due to differences in expression in the case of MMP-9 but not in the case of MMP-2. This observation is corroborated by the fact that correlated changes of expression in a regulator of MMP-2, TIMP-2, were also seen. Conclusion This work identifies a specific effect of IGF-1 on the invasive capacity of DU145 prostate cancer cells, and furthermore delineates mechanisms that contribute to this effect. PMID:18598360

  7. Role of IGF-1/IGF-1R in regulation of invasion in DU145 prostate cancer cells

    Directory of Open Access Journals (Sweden)

    Setya Hemani

    2008-07-01

    Full Text Available Abstract Background Prostate cancer progression to androgen independence is the primary cause of mortality by this tumor type. The IGF-1/IGF-1R axis is well known to contribute to prostate cancer initiation, but its contribution to invasiveness and the downstream signalling mechanisms that are involved are unclear at present. Results We examined the invasive response of androgen independent DU145 prostate carcinoma cells to IGF-1 stimulation using Matrigel assays. We then examined the signaling mechanisms and protease activities that are associated with this response. IGF-1 significantly increased the invasive capacity of DU145 cells in vitro, and this increase was inhibited by blocking IGF-1R. We further demonstrated that specific inhibitors of the MAPK and PI3-K pathways decrease IGF-1-mediated invasion. To determine potential molecular mechanisms for this change in invasive capacity, we examined changes in expression and activity of matrix metalloproteinases. We observed that IGF-1 increases the enzymatic activity of MMP-2 and MMP-9 in DU145 cells. These changes in activity are due to differences in expression in the case of MMP-9 but not in the case of MMP-2. This observation is corroborated by the fact that correlated changes of expression in a regulator of MMP-2, TIMP-2, were also seen. Conclusion This work identifies a specific effect of IGF-1 on the invasive capacity of DU145 prostate cancer cells, and furthermore delineates mechanisms that contribute to this effect.

  8. Synthesis of 1,4,5 trisubstituted γ-lactams via a 3-component cascade reaction

    DEFF Research Database (Denmark)

    Petersen, Michael Åxman; Mortensen, Michael Agerup; Cohrt, A. Emil;

    2015-01-01

    A three component one-pot cascade reaction was developed for the synthesis of 1,4,5-trisubstituted γ-lactams. The resulting scaffold can be modified independently at three positions, two of which are conveniently accessed by changing the components of the one-pot reaction. The phases of building ...

  9. 12 CFR 563.145 - May I combine my notice or application with other notices or applications?

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false May I combine my notice or application with..., DEPARTMENT OF THE TREASURY SAVINGS ASSOCIATIONS-OPERATIONS Capital Distributions § 563.145 May I combine my notice or application with other notices or applications? You may combine the notice or...

  10. Clinical features and viral quasispecies characteristics associated with infection by the hepatitis B virus G145R immune escape mutant.

    Science.gov (United States)

    Xue, Yuan; Wang, Ming-Jie; Yang, Zhi-Tao; Yu, De-Min; Han, Yue; Huang, Dao; Zhang, Dong-Hua; Zhang, Xin-Xin

    2017-03-22

    Coexistence of the hepatitis B surface antigen (HBsAg) and hepatitis B surface antibody (anti-HBs) is an uncommon phenomenon, and the underlying mechanisms remain largely unknown. Amino-acid (aa) substitution from glycine to arginine at aa 145 (G145R), in the major hydrophilic region, has been reported in patients with HBsAg and anti-HBs coexistence. However, there is limited knowledge about the clinical features and viral quasispecies characteristics associated with G145R mutant hepatitis B virus (HBV) infection. We herein describe the dynamic changes in the serological and virological markers in a case of hepatitis B with coexisting HBsAg and anti-HBs, caused by a G145R immune escape mutant (genotype C). Entecavir was administered during the 4th week after admission. Alanine aminotransferase peaked in the 16th week, while both the HBsAg and HBeAg declined rapidly. HBsAg clearance and hepatitis B e antigen (HBeAg)/hepatitis B e antibody (anti-HBe) seroconversion were achieved in the 36th week, and then entecavir was withdrawn. A follow-up of 96 weeks showed that HBV DNA remained undetectable and that anti-HBs was maintained above 100 mIU/mL. The quasispecies characteristics of the G145R mutant HBV were investigated via ultra-deep sequencing. The complexity and genetic distance of the S and RT regions were much higher in the 8th week than at baseline or in the 4th week. Moreover, the frequencies of mutations (L173P, Q181R and A184V) in cytotoxic T lymphocyte epitopes increased before entecavir treatment. These findings extend understanding of the evolution of HBV under host immune pressure and of the clinical outcomes of affected patients.

  11. MiR-145 is downregulated in human ovarian cancer and modulates cell growth and invasion by targeting p70S6K1 and MUC1

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Huijuan [Department of Gynecological Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060 (China); Xiao, ZhengHua [Department of gynecology, Yongchuan Affiliated Hospital of Chongqing Medical University, Chongqing City 404100 (China); Wang, Ke; Liu, Wenxin [Department of Gynecological Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060 (China); Hao, Quan, E-mail: quanhao2002@163.com [Department of Gynecological Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060 (China)

    2013-11-29

    Highlights: •MiR-145 is downregulated in human ovarian cancer. •MiR-145 targets p70S6K1 and MUC1. •p70S6K1 and MUC1 are involved in miR-145 mediated tumor cell growth and cell invasion, respectively. -- Abstract: MicroRNAs (miRNAs) are a family of small non-coding RNA molecules that regulate gene expression at post-transcriptional levels. Previous studies have shown that miR-145 is downregulated in human ovarian cancer; however, the roles of miR-145 in ovarian cancer growth and invasion have not been fully demonstrated. In the present study, Northern blot and qRT-PCR analysis indicate that miR-145 is downregulated in ovarian cancer tissues and cell lines, as well as in serum samples of ovarian cancer, compared to healthy ovarian tissues, cell lines and serum samples. Functional studies suggest that miR-145 overexpression leads to the inhibition of colony formation, cell proliferation, cell growth viability and invasion, and the induction of cell apoptosis. In accordance with the effect of miR-145 on cell growth, miR-145 suppresses tumor growth in vivo. MiR-145 is found to negatively regulate P70S6K1 and MUC1 protein levels by directly targeting their 3′UTRs. Importantly, the overexpression of p70S6K1 and MUC1 can restore the cell colony formation and invasion abilities that are reduced by miR-145, respectively. MiR-145 expression is increased after 5-aza-CdR treatment, and 5-aza-CdR treatment results in the same phenotype as the effect of miR-145 overexpression. Our study suggests that miR-145 modulates ovarian cancer growth and invasion by suppressing p70S6K1 and MUC1, functioning as a tumor suppressor. Moreover, our data imply that miR-145 has potential as a miRNA-based therapeutic target for ovarian cancer.

  12. Correction to: Biores Open Access 2016;5:137–145

    Directory of Open Access Journals (Sweden)

    Walter H. Moos

    2017-05-01

    Full Text Available In the 2016 volume of BioResearch Open Access (vol. 5, no. 1; 137–145, the article entitled “Microbiota and Neurological Disorders: A Gut Feeling” by Moos et al. requires a correction. Reference 98 was incorrectly cited in the first paragraph of page 142; this should be reference 194, which was inadvertently omitted from the article. The corrected text and newly added reference are below: The mouth, being an extension of the digestive tract, presents a readily accessible diagnostic site for the early detection of potential unhealthy pathogens resident in the gut. Salivanomics is a rapidly emerging tool in the arsenal of salivary diagnostics.194 Collecting saliva or swabbing the inside cheek of the mouth is noninterventional, making it a highly attractive diagnostic procedure, particularly for infants and young children. It is not unlikely that regular visits to your dentist may soon be as important to your gut as it is to your oral hygiene, white teeth, and a nice smile. 194. Zhang Y, Sun J, Lin CC, et al. The emerging landscape of salivary diagnostics. Periodontol 2000. 2016;70:38–52. The article has been corrected to reflect this change. The authors apologize for this error.

  13. Molecular cloning, purification, and biochemical characterization of recombinant isocitrate dehydrogenase from Streptomyces coelicolor M-145.

    Science.gov (United States)

    Takahashi-Iñiguez, Tóshiko; Cruz-Rabadán, Saul; Burciaga-Cifuentes, Luis Miguel; Flores, María Elena

    2014-01-01

    Isocitrate dehydrogenase is a key enzyme in carbon metabolism. In this study we demonstrated that SCO7000 of Streptomyces coelicolor M-145 codes for the isocitrate dehydrogenase. Recombinant enzyme expressed in Escherichia coli had a specific activity of 25.3 μmoles/mg/min using NADP(+) and Mn(2+) as a cofactor, 40-times higher than that obtained in cell-free extract. Pure IDH showed a single band with an apparent Mr of 84 KDa in SDS-PAGE, which was also recognized as His-tag protein in the Western blot. Unexpectedly, in ND-PAGE conditions showed a predominant band of ~168 KDa that corresponded to the dimeric form of ScIDH. Also, zymogram assay and analytical gel filtration reveal that dimer was the active form. Kinetic parameters were 1.38, 0.11, and 0.109 mM for isocitrate, NADP, and Mn(2+), respectively. ATP, ADP, AMP, and their mixtures were the main ScIDH activity inhibitors. Zn(2+), Mg(2+), Ca(2+), and Cu(+) had inhibitory effect on enzyme activity.

  14. Norcantharidin induced DU145 cell apoptosis through ROS-mediated mitochondrial dysfunction and energy depletion.

    Science.gov (United States)

    Shen, Bo; He, Pei-Jie; Shao, Chun-Lin

    2013-01-01

    Norcantharidin (NCTD), a demethylated analog of cantharidin derived from blister beetles, has attracted considerable attentions in recent years due to their definitely toxic properties and the noteworthy advantages in stimulating bone marrow and increasing the peripheral leukocytes. Hence, it is worth studying the anti-tumor effect of NCTD on human prostate cancer cells DU145. It was found that after the treatment of NCTD with different concentrations (25-100 μM), the cell proliferation was significantly inhibited, which led to the appearance of micronucleus (MN). Moreover, the cells could be killed in a dose-/time-dependent manner along with the reduction of PCNA (proliferating cell nuclear antigen) expression, destruction of mitochondrial membrane potential (MMP), down-regulation of MnSOD, induction of ROS, depletion of ATP, and activation of AMPK (Adenosine 5'-monophosphate -activated protein kinase) . In addition, a remarkable release of cytochrome c was found in the cells exposed to 100 μM NCTD and exogenous SOD-PEG could eliminate the generation of NCTD-induced MN. In conclusion, our studies indicated that NCTD could induce the collapse of MMP and mitochondria dysfunction. Accumulation of intercellular ROS could eventually switch on the apoptotic pathway by causing DNA damage and depleting ATP.

  15. Norcantharidin induced DU145 cell apoptosis through ROS-mediated mitochondrial dysfunction and energy depletion.

    Directory of Open Access Journals (Sweden)

    Bo Shen

    Full Text Available Norcantharidin (NCTD, a demethylated analog of cantharidin derived from blister beetles, has attracted considerable attentions in recent years due to their definitely toxic properties and the noteworthy advantages in stimulating bone marrow and increasing the peripheral leukocytes. Hence, it is worth studying the anti-tumor effect of NCTD on human prostate cancer cells DU145. It was found that after the treatment of NCTD with different concentrations (25-100 μM, the cell proliferation was significantly inhibited, which led to the appearance of micronucleus (MN. Moreover, the cells could be killed in a dose-/time-dependent manner along with the reduction of PCNA (proliferating cell nuclear antigen expression, destruction of mitochondrial membrane potential (MMP, down-regulation of MnSOD, induction of ROS, depletion of ATP, and activation of AMPK (Adenosine 5'-monophosphate -activated protein kinase . In addition, a remarkable release of cytochrome c was found in the cells exposed to 100 μM NCTD and exogenous SOD-PEG could eliminate the generation of NCTD-induced MN. In conclusion, our studies indicated that NCTD could induce the collapse of MMP and mitochondria dysfunction. Accumulation of intercellular ROS could eventually switch on the apoptotic pathway by causing DNA damage and depleting ATP.

  16. New 145-MHz Source Measurements by PAPER in the Southern Sky

    CERN Document Server

    Jacobs, Daniel C; Parsons, Aaron R; Pober, Jonathan C; Bradley, Richard F; Carilli, Chris L; Gugliucci, Nicole E; Manley, Jason R; van der Merwe, Carel; Moore, David F; Parashare, Chaitali R

    2011-01-01

    We present observations from the Precision Array for Probing the Epoch of Reionization (PAPER) in South Africa, observed in May and September 2010. Using two nights of drift scanning with PAPER's 60\\arcdeg\\ FWHM beam we have made a map covering the entire sky below +10 degrees declination with an effective center frequency of 145 MHz, a 70-MHz bandwidth, and a resolution of 26\\arcmin. A 4800 square-degree region of this large map with the lowest Galactic emission reaches an RMS of 0.7 Jy. We establish an absolute flux scale using sources from the 160-MHz Culgoora catalog. Using the 408-MHz Molonglo Reference Catalog (MRC) as a finding survey, we identify counterparts to 480 sources in our maps, and compare our fluxes to the MRC and to 332 sources in the Culgoora catalog. For both catalogs, the ratio of PAPER to catalog flux averages to 1, with a standard deviation of 50%. This measured variation is consistent with comparisons between independent catalogs observed at different bands. The PAPER data represent n...

  17. Induction of inositol 1,4,5 trisphosphate receptor genes by ionizing radiation

    Energy Technology Data Exchange (ETDEWEB)

    Yan, J. [Queensland Inst. of Medical Research, Brisbane, QLD (Australia)]|[Queensland Univ., Brisbane, QLD (Australia). Dept. of Surgery; Khanna, K.K.; Lavin, M.F. [Queensland Inst. of Medical Research, Brisbane, QLD (Australia)

    1996-05-01

    We used differential display, a method designed to amplify partial cDNA sequences from subsets of mRNAs, to identify mRNAs induced by ionizing radiation in human Epstein Barr Virus (EBV)-transformed lymphoblastoid cells. Increased expression of a cDNA corresponding to the inositol 1,4,5 trisphosphate receptor (InsP{sub 3}R) type 1 was observed after exposure of cells to 3Gy {gamma}-rays. This was confirmed by Northern blot analysis. The increase in mRNA for InsP{sub 3}R type 1 was accompanied by a corresponding increase in the level of InsP{sub 3}R type 1 protein as determined by Western blotting. Exposure of cells from patients with the human genetic disorder ataxia-telangiectasia (A-T), characterized by hypersensitivity to ionizing radiation, failed to change the levels of InsP{sub 3}R type 1 mRNA and, as expected, there was no increase in InsP{sub 3}R type 1 protein in A-T cells in response to radiation exposure. Protein levels for two other InsP{sub 3}Rs, types 2 and 3, were observed to increase in control and A-T cells after exposure to ionizing radiation. The induction of the InsP{sub 3}R type 1, which is primarily located in the endoplasmic reticulum, may play an important role in radiation signal transduction. (Author).

  18. Neutron star crustal cooling in KS 1731-260 14.5 years into quiescence

    CERN Document Server

    Merritt, Rachael L; Brown, Edward F; Page, Dany; Cumming, Andrew; Degenaar, Nathalie; Deibel, Alex; Homan, Jeroen; Miller, Jon M; Wijnands, Rudy

    2016-01-01

    Crustal cooling of accretion-heated neutron stars provides insight into the stellar interior of neutron stars. The neutron star X-ray transient, KS 1731-260, was in outburst for 12.5 years before returning to quiescence in 2001. We have monitored the cooling of this source since then through Chandra and XMM-Newton observations. Here, we present a 150 ks Chandra observation of KS 1731-260 taken in August 2015, about 14.5 years into quiescence, and 6 years after the previous observation. We find that the neutron star surface temperature is consistent with the previous observation, suggesting that crustal cooling has likely stopped and the crust has reached thermal equilibrium with the core. Using a theoretical crust thermal evolution code, we fit the observed cooling curves and constrain the core temperature (T$_c = 9.35\\pm0.25\\times10^7$ K), composition (Q$_{imp} = 4.4^{+2.2}_{-0.5}$) and level of extra shallow heating required (Q$_{sh} = 1.36\\pm0.18$ MeV/nucleon). We find that the presence of a low thermal co...

  19. The inositol 1,4,5-trisphosphate receptor regulates autophagy through its interaction with Beclin 1.

    Science.gov (United States)

    Vicencio, J M; Ortiz, C; Criollo, A; Jones, A W E; Kepp, O; Galluzzi, L; Joza, N; Vitale, I; Morselli, E; Tailler, M; Castedo, M; Maiuri, M C; Molgó, J; Szabadkai, G; Lavandero, S; Kroemer, G

    2009-07-01

    The inositol 1,4,5-trisphosphate receptor (IP(3)R) is a major regulator of apoptotic signaling. Through interactions with members of the Bcl-2 family of proteins, it drives calcium (Ca(2+)) transients from the endoplasmic reticulum (ER) to mitochondria, thereby establishing a functional and physical link between these organelles. Importantly, the IP(3)R also regulates autophagy, and in particular, its inhibition/depletion strongly induces macroautophagy. Here, we show that the IP(3)R antagonist xestospongin B induces autophagy by disrupting a molecular complex formed by the IP(3)R and Beclin 1, an interaction that is increased or inhibited by overexpression or knockdown of Bcl-2, respectively. An effect of Beclin 1 on Ca(2+) homeostasis was discarded as siRNA-mediated knockdown of Beclin 1 did not affect cytosolic or luminal ER Ca(2+) levels. Xestospongin B- or starvation-induced autophagy was inhibited by overexpression of the IP(3)R ligand-binding domain, which coimmunoprecipitated with Beclin 1. These results identify IP(3)R as a new regulator of the Beclin 1 complex that may bridge signals converging on the ER and initial phagophore formation.

  20. Sensitive and label-free detection of miRNA-145 by triplex formation.

    Science.gov (United States)

    Aviñó, Anna; Huertas, César S; Lechuga, Laura M; Eritja, Ramon

    2016-01-01

    The development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the diagnostic field. miRNA profiles depend greatly on the sample and the analytical platform employed, leading sometimes to contradictory results. In this work, we study the use of modified parallel tail-clamps to detect a miRNA sequence involved in tumor suppression by triplex formation. Thermal denaturing curves and circular dichroism (CD) measurements have been performed to confirm that parallel clamps carrying 8-aminoguanine form the most stable triplex structures with their target miRNA. The modified tail-clamps have been tested as bioreceptors in a surface plasmon resonance (SPR) biosensor for the detection of miRNA-145. The detection limit was improved 2.4 times demonstrating that a stable triplex structure is formed between target miRNA and 8-aminoguanine tail-clamp bioreceptor. This new approach is an essential step toward the label-free and reliable detection of miRNA signatures for diagnostic purposes.

  1. Roles of inositol 1,4,5-trisphosphate receptors in spinocerebellar ataxias.

    Science.gov (United States)

    Tada, Masayoshi; Nishizawa, Masatoyo; Onodera, Osamu

    2016-03-01

    Modulation of the intracellular calcium concentration is a ubiquitous signaling system involved in the control of numerous biological processes in a wide variety of cells. Inositol 1,4,5-trisphosphate (IP3) receptors (IP3Rs), which act as calcium release channels in the ER membrane, play a key role in the regulation of intracellular calcium concentration. IP3R type 1 (IP3R1) is the major neuronal IP3R isoform in the central nervous system and particularly abundant in cerebellar Purkinje cells. Heterozygous deletions or missense mutations in ITPR1, which encodes IP3R1, result in autosomal dominantly inherited spinocerebellar ataxias (SCAs), including SCA types 15 (SCA15) and 29 (SCA29). In addition, homozygous missense mutations in carbonic anhydrase-related protein VIII (CARP), which suppresses the ability of IP3 to bind to IP3R1, cause a recessively inherited ataxia with mild cognitive impairment with/without quadrupedal gait. Moreover, cytosolic calcium overload with excessive IP3R1 activity has been implicated in the pathogenesis of other SCAs, including SCA types 2 (SCA2) and 3 (SCA3). These facts indicate that dysregulation of IP3R-mediated calcium signaling is linked to the pathogenesis of SCAs. Here, we focus on the molecular basis of SCA15 and SCA29, which are caused by mutations in ITPR1. In addition, we discuss other SCAs whose pathogenesis may be linked to aberrant activation of IP3R-mediated Ca(2+) signaling.

  2. Design for the 145MW blast furnance gas firing gas turbine combined cycle plant

    Energy Technology Data Exchange (ETDEWEB)

    Takano, H. (Kawasaki Steel Corp., Chiba (Japan). Technical Research Lab.); Kitauchi, Y.; Hiura, H. (Mitsubishi Heavy Industries Ltd., Takasago, Hyogo (Japan). Takasago Works)

    1988-01-01

    A 145 MW blast furnance gas firing gas turbine combined cycle plant was designed and installed in a steel works of Japan as a repowering unit. A 124 MW large scale gas turbine with turbine inlet temperature 1150{sup {degrees}}C (1423 K) was adopted as a core engine for the combined cycle plant. The fuel of this gas turbine is blast furnace gas mixed with coke oven gas which are byproducts in steel works, and the calorific value of the mixed gas to be controlled is about 1000 kcal/Nm{sup 3} (4187 kJ/Nm{sup 3}). A specially designed multi-cannular type combustor was developed to burn such a low BTV fuel. The gas turbine, generator, steam turbine and fuel gas compressor are connected to make a single shaft configuration. As a result of this introducing the gas turbine combined cycle plant, the plant thermal efficiency was achieved above 45% (at NET) and the total electricity generation in the works is increased from 243 MW to 317 MW. This paper describes the design features of this combined cycle plant.

  3. Electrical and dielectric properties of irradiated KU1 quartz glass from DC to 145 GHz

    CERN Document Server

    Vila, R; Heidinger, R; Morono, A; Hodgson, E R

    2002-01-01

    To characterize and examine the possible use of the KU1 quartz glass for both diagnostic and remote handling applications, the radiation induced conductivity and electrical degradation (RIC and RIED), together with dielectric loss and permittivity (10 mHz-145 GHz) have been determined for as-received, electron and neutron irradiated material. Results show that the RIC is extremely low (<10 sup - sup 8 S/m compared with about 10 sup - sup 7 S/m) for candidate oxide materials at the same dose rate, but markedly increases for doses up to about 3x10 sup - sup 5 dpa while the conductivity without radiation is reduced with dose. Dielectric measurements from 1 kHz to 20 GHz during electron irradiation show that prompt effects are only observed at very low frequencies, consistent with the low RIC results. For neutron irradiation to 10 sup - sup 4 dpa, post-irradiation measurements indicate a permanent increase in loss by up to a factor of 2 below 60 MHz. No effect is observed in the GHz range.

  4. 001×14.5离子交换树脂对镍(Ⅱ)的吸附%Adsorption of Ni(Ⅱ) by ion exchange resin(001 × 14.5)

    Institute of Scientific and Technical Information of China (English)

    杨金杯; 陈玉成; 余美琼; 邱挺

    2013-01-01

    The adsorption of Ni(Ⅱ) in aqueous solution with ion exchange resins was investigated,and 001 × 14.5 strong acidic cation ion exchange resin showed the maximum capacity by the resin selection experiment.The adsorption properties of the selected 001 × 14.5 resin for Ni(Ⅱ) were studied by static experiments.The results indicated that the influence of external diffusion was eliminated when stirring speed was over 100 r/ min,and the best pH value was 7,and that the percent removal of Ni(Ⅱ) increased as the consumption of resin.The adsorption behavior of 001 × 14.5 for Ni (Ⅱ),which was the favourable adsorption,conformed the Langmuir isotherm equation well.The adsorption process corresponded with pseudo-second-order kinetic model,the activation energy was 30.9 kJ/mol,and the kinetics experiments showed that intrapartical diffusion was the main rate-controlling step.The desorption efficiency of resin to Ni(Ⅱ) could be over 98% with 1 mol/L sulfuric acid to regenerate the saturated resin.%实验采用离子交换树脂法吸附镍(Ⅱ),树脂选型确定了强酸性阳离子交换树脂001×14.5对镍(Ⅱ)吸附容量最大.用所选的001×14.5树脂吸附镍(Ⅱ)的过程,静态吸附实验表明,转速大于100 r/min时,对树脂吸附的影响可忽略,即基本消除外扩散,pH =7.0时吸附最佳,镍(Ⅱ)吸附率随树脂用量的增加而增大;001×14.5树脂吸附镍(Ⅱ)过程符合Langmuir等温吸附方程,且为优惠吸附;吸附过程符合拟二级动力学模型,吸附过程活化能为E=30.9 kJ/mol,由颗粒内扩散控制;用1 mol/L的硫酸对吸附饱和树脂进行脱附再生,脱附率可达98%以上.

  5. 7 CFR 1956.145 - Disposition of essential FmHA or its successor agency under Public Law 103-354 records.

    Science.gov (United States)

    2010-01-01

    ... under Public Law 103-354 records. 1956.145 Section 1956.145 Agriculture Regulations of the Department of... successor agency under Public Law 103-354 records. FmHA or its successor agency under Public Law 103-354 Instruction 2033-A (available in any FmHA or its successor agency under Public Law 103-354 office)...

  6. 14.5GHz紧凑型、低成本、全永磁ECR离子源%Compact,Low-Cost,14.5GHz All-Permanent Magnet Field ECR Ion Source

    Institute of Scientific and Technical Information of China (English)

    E.Galutschek; E.Salzborn; F.Aumayr; HP.Winter

    2007-01-01

    A compact 14.5GHz electron cyclotron resonance(ECR)ion source for the production of slow,multiply charged ions has been constructed,with the plasma-confining magnetic field produced exclusively by permanent magnets.Microwave power of up to 175W in the frequency range from 12.75 to 14.5GHz is transmitted from ground potential via a PTFE window into the water-cooled plasma chamber which can be equipped with an aluminum liner.The waveguide coupling system serves also as biased electrode,and two remotely-controlled gas inlet valves connected via an insulating break permit plasma operation in the gasmixing mode.A triode extraction system sustains ion acceleration voltages between lkV and 10kV.The ECR ion sonrce is fully computer-controlled and can be remotely operated from any desired location via Ethernet.

  7. Functional inositol 1,4,5-trisphosphate receptors assembled from concatenated homo- and heteromeric subunits.

    Science.gov (United States)

    Alzayady, Kamil J; Wagner, Larry E; Chandrasekhar, Rahul; Monteagudo, Alina; Godiska, Ronald; Tall, Gregory G; Joseph, Suresh K; Yule, David I

    2013-10-11

    Vertebrate genomes code for three subtypes of inositol 1,4,5-trisphosphate (IP3) receptors (IP3R1, -2, and -3). Individual IP3R monomers are assembled to form homo- and heterotetrameric channels that mediate Ca(2+) release from intracellular stores. IP3R subtypes are regulated differentially by IP3, Ca(2+), ATP, and various other cellular factors and events. IP3R subtypes are seldom expressed in isolation in individual cell types, and cells often express different complements of IP3R subtypes. When multiple subtypes of IP3R are co-expressed, the subunit composition of channels cannot be specifically defined. Thus, how the subunit composition of heterotetrameric IP3R channels contributes to shaping the spatio-temporal properties of IP3-mediated Ca(2+) signals has been difficult to evaluate. To address this question, we created concatenated IP3R linked by short flexible linkers. Dimeric constructs were expressed in DT40-3KO cells, an IP3R null cell line. The dimeric proteins were localized to membranes, ran as intact dimeric proteins on SDS-PAGE, and migrated as an ∼1100-kDa band on blue native gels exactly as wild type IP3R. Importantly, IP3R channels formed from concatenated dimers were fully functional as indicated by agonist-induced Ca(2+) release. Using single channel "on-nucleus" patch clamp, the channels assembled from homodimers were essentially indistinguishable from those formed by the wild type receptor. However, the activity of channels formed from concatenated IP3R1 and IP3R2 heterodimers was dominated by IP3R2 in terms of the characteristics of regulation by ATP. These studies provide the first insight into the regulation of heterotetrameric IP3R of defined composition. Importantly, the results indicate that the properties of these channels are not simply a blend of those of the constituent IP3R monomers.

  8. Proteolytic fragmentation of inositol 1,4,5-trisphosphate receptors: a novel mechanism regulating channel activity?

    Science.gov (United States)

    Wang, Liwei; Alzayady, Kamil J; Yule, David I

    2016-06-01

    Inositol 1,4,5-trisphosphate receptors (IP3 Rs) are a family of ubiquitously expressed intracellular Ca(2+) release channels. Regulation of channel activity by Ca(2+) , nucleotides, phosphorylation, protein binding partners and other cellular factors is thought to play a major role in defining the specific spatiotemporal characteristics of intracellular Ca(2+) signals. These properties are, in turn, believed pivotal for the selective and specific physiological activation of Ca(2+) -dependent effectors. IP3 Rs are also substrates for the intracellular cysteine proteases, calpain and caspase. Cleavage of the IP3 R has been proposed to play a role in apoptotic cell death by uncoupling regions important for IP3 binding from the channel domain, leaving an unregulated leaky Ca(2+) pore. Contrary to this hypothesis, we demonstrate following proteolysis that N- and C-termini of IP3 R1 remain associated, presumably through non-covalent interactions. Further, we show that complementary fragments of IP3 R1 assemble into tetrameric structures and retain their ability to be regulated robustly by IP3 . While peptide continuity is clearly not necessary for IP3 -gating of the channel, we propose that cleavage of the IP3 R peptide chain may alter other important regulatory events to modulate channel activity. In this scenario, stimulation of the cleaved IP3 R may support distinct spatiotemporal Ca(2+) signals and activation of specific effectors. Notably, in many adaptive physiological events, the non-apoptotic activities of caspase and calpain are demonstrated to be important, but the substrates of the proteases are poorly defined. We speculate that proteolytic fragmentation may represent a novel form of IP3 R regulation, which plays a role in varied adaptive physiological processes.

  9. The neutron electric form factor to Q² = 1.45 (GeV/c)²

    Energy Technology Data Exchange (ETDEWEB)

    Bradley Plaster

    2004-02-01

    The nucleon elastic electromagnetic form factors are fundamental quantities needed for an understanding of nucleon and nuclear electromagnetic structure. The evolution of the Sachs electric and magnetic form factors with Q2, the square of the four-momentum transfer, is related to the distribution of charge and magnetization within the nucleon. High precision measurements of the nucleon form factors are essential for stringent tests of our current theoretical understanding of confinement within the nucleon. Measurements of the neutron form factors, in particular, those of the neutron electric form factor, have been notoriously difficult due to the lack of a free neutron target and the vanishing integral charge of the neutron. Indeed, a precise measurement of the neutron electric form factor has eluded experimentalists for decades; however, with the advent of high duty-factor polarized electron beam facilities, experiments employing polarization degrees of freedom have finally yielded the first precise measurements of this fundamental quantity. Following a general overview of the experimental and theoretical status of the nucleon form factors, a detailed description of an experiment designed to extract the neutron electric form factor from measurements of the neutron's recoil polarization in quasielastic 2H(e, e')1H scattering is presented. The experiment described here employed the Thomas Jefferson National Accelerator Facility's longitudinally polarized electron beam, a magnetic spectrometer for detection of the scattered electron, and a neutron polarimeter designed specifically for this experiment. Measurements were conducted at three Q2 values of 0.45, 1.13, and 1.45 (GeV/c)2, and the final results extracted from an analysis of the data acquired in this experiment are reported and compared with recent theoretical predictions for the nucleon form factors.

  10. Hydrogen peroxide down-regulates inositol 1,4,5-trisphosphate receptor content through proteasome activation.

    Science.gov (United States)

    Martín-Garrido, A; Boyano-Adánez, M C; Alique, M; Calleros, L; Serrano, I; Griera, M; Rodríguez-Puyol, D; Griendling, K K; Rodríguez-Puyol, M

    2009-11-15

    Hydrogen peroxide (H(2)O(2)) is implicated in the regulation of signaling pathways leading to changes in vascular smooth muscle function. Contractile effects produced by H(2)O(2) are due to the phosphorylation of myosin light chain kinase triggered by increases in intracellular calcium (Ca(2+)) from intracellular stores or influx of extracellular Ca(2+). One mechanism for mobilizing such stores involves the phosphoinositide pathway. Inositol 1,4,5-trisphosphate (IP(3)) mobilizes intracellular Ca(2+) by binding to a family of receptors (IP(3)Rs) on the endoplasmic-sarcoplasmic reticulum that act as ligand-gated Ca(2+) channels. IP(3)Rs can be rapidly ubiquitinated and degraded by the proteasome, causing a decrease in cellular IP(3)R content. In this study we show that IP(3)R(1) and IP(3)R(3) are down-regulated when vascular smooth muscle cells (VSMC) are stimulated by H(2)O(2), through an increase in proteasome activity. Moreover, we demonstrate that the decrease in IP(3)R by H(2)O(2) is accompanied by a reduction in calcium efflux induced by IP(3) in VSMC. Also, we observed that angiotensin II (ANGII) induces a decrease in IP(3)R by activation of NADPH oxidase and that preincubation with H(2)O(2) decreases ANGII-mediated calcium efflux and planar cell surface area in VSMC. The decreased IP(3) receptor content observed in cells was also found in aortic rings, which exhibited a decreased ANGII-dependent contraction after treatment with H(2)O(2). Altogether, these results suggest that H(2)O(2) mediates IP(3)R down-regulation via proteasome activity.

  11. The neutron electric form factor to Q² = 1.45 (GeV/c)²

    Energy Technology Data Exchange (ETDEWEB)

    Plaster, Bradley [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

    2004-02-01

    The nucleon elastic electromagnetic form factors are fundamental quantities needed for an understanding of nucleon and nuclear electromagnetic structure. The evolution of the Sachs electric and magnetic form factors with Q2, the square of the four-momentum transfer, is related to the distribution of charge and magnetization within the nucleon. High precision measurements of the nucleon form factors are essential for stringent tests of our current theoretical understanding of confinement within the nucleon. Measurements of the neutron form factors, in particular, those of the neutron electric form factor, have been notoriously difficult due to the lack of a free neutron target and the vanishing integral charge of the neutron. Indeed, a precise measurement of the neutron electric form factor has eluded experimentalists for decades; however, with the advent of high duty-factor polarized electron beam facilities, experiments employing polarization degrees of freedom have finally yielded the first precise measurements of this fundamental quantity. Following a general overview of the experimental and theoretical status of the nucleon form factors, a detailed description of an experiment designed to extract the neutron electric form factor from measurements of the neutron's recoil polarization in quasielastic 2H(e, e')1H scattering is presented. The experiment described here employed the Thomas Jefferson National Accelerator Facility's longitudinally polarized electron beam, a magnetic spectrometer for detection of the scattered electron, and a neutron polarimeter designed specifically for this experiment. Measurements were conducted at three Q2 values of 0.45, 1.13, and 1.45 (GeV/c)2, and the final results extracted from an analysis of the data acquired in this experiment are reported and compared with recent theoretical predictions for the nucleon form factors.

  12. Inositol-1,4,5-trisphosphate receptor regulates hepatic gluconeogenesis in fasting and diabetes.

    Science.gov (United States)

    Wang, Yiguo; Li, Gang; Goode, Jason; Paz, Jose C; Ouyang, Kunfu; Screaton, Robert; Fischer, Wolfgang H; Chen, Ju; Tabas, Ira; Montminy, Marc

    2012-04-08

    In the fasted state, increases in circulating glucagon promote hepatic glucose production through induction of the gluconeogenic program. Triggering of the cyclic AMP pathway increases gluconeogenic gene expression via the de-phosphorylation of the CREB co-activator CRTC2 (ref. 1). Glucagon promotes CRTC2 dephosphorylation in part through the protein kinase A (PKA)-mediated inhibition of the CRTC2 kinase SIK2. A number of Ser/Thr phosphatases seem to be capable of dephosphorylating CRTC2 (refs 2, 3), but the mechanisms by which hormonal cues regulate these enzymes remain unclear. Here we show in mice that glucagon stimulates CRTC2 dephosphorylation in hepatocytes by mobilizing intracellular calcium stores and activating the calcium/calmodulin-dependent Ser/Thr-phosphatase calcineurin (also known as PP3CA). Glucagon increased cytosolic calcium concentration through the PKA-mediated phosphorylation of inositol-1,4,5-trisphosphate receptors (InsP(3)Rs), which associate with CRTC2. After their activation, InsP(3)Rs enhanced gluconeogenic gene expression by promoting the calcineurin-mediated dephosphorylation of CRTC2. During feeding, increases in insulin signalling reduced CRTC2 activity via the AKT-mediated inactivation of InsP(3)Rs. InsP(3)R activity was increased in diabetes, leading to upregulation of the gluconeogenic program. As hepatic downregulation of InsP(3)Rs and calcineurin improved circulating glucose levels in insulin resistance, these results demonstrate how interactions between cAMP and calcium pathways at the level of the InsP(3)R modulate hepatic glucose production under fasting conditions and in diabetes.

  13. 医学期刊学术不端行为145例分析%The analysis of 145 cases of improper avademic behavior in medical journal

    Institute of Scientific and Technical Information of China (English)

    贾安; 张颖

    2015-01-01

    Objective To understand the status of the improper avademic behavior in medical journal,and to explore the prevention measures.Methods Total of 1 321 article in Zhongguo Yishi Jinxiu Zazhi were included in this study.Similarity detection system of Wanfang data and Google and baidu search engine were used to duplicate checking.The suspected existence of contributions for the improper avademic behavior,to check the whole paper of relevant original manuscript,and analyzed the characteristics of improper avademic behavior.Results One hundred and forty-five improper avademic behavior were found in 1 321 articles,accounted for 11.0%.The similarity of 58 paper were higer than 50.00%,accounted for 40.0%.Among them,the similarity of 10 papers were more than 90.00%,8 papers were 80.00%-89.99%,12 papers were 70.00%-79.99%,13 papers were 60.00%-69.99%,15 papers were 50.00%-59.99%,13 papers were 40.00%-49.99%,accounted for 9.0%,24 papers were 30.00%-39.99%,accounted for 16.6%,50 papers were 20.00%-29.99%,accounted for 34.5%.In 145 papers,copying accounted for 35.2% (51/145),data forge or tamper accounted for 28.3%(41/145),breach of medical ethics and academic moral accounted for 28.3% (41/145),fraud accounted for 6.9% (10/145),a draft cast more accounted for 1.4% (2/145).Original articles in the clinic were 92 papers,accounted for 63.4%;case reports were 28 papers,accounted for 19.3%;review were 25 papers,accounted for 17.2%.76 papers with a single author,accounted for 52.4%;38 papers with two authors,accounted for 26.2%,31 papers with more than three authors,accounted for 21.4%.Conclusions Contributions of suspected improper avademic behavior ratio is higher,and have certin characteristic.Medical journal editors should strengthen the responsibility consciousness,improve the ability to identify misconduct papers.%目的 了解医学期刊学术不端行为的状况,探讨防范学术不端行为的措施.方法

  14. Capsaicin-induced genotoxic stress does not promote apoptosis in A549 human lung and DU145 prostate cancer cells.

    Science.gov (United States)

    Lewinska, Anna; Jarosz, Paulina; Czech, Joanna; Rzeszutek, Iwona; Bielak-Zmijewska, Anna; Grabowska, Wioleta; Wnuk, Maciej

    2015-02-01

    Capsaicin is the major pungent component of the hot chili peppers of the genus Capsicum, which are consumed worldwide as a food additive. More recently, the selective action of capsaicin against cancer cells has been reported. Capsaicin was found to induce apoptosis and inhibit proliferation of a wide range of cancer cells in vitro, whereas being inactive against normal cells. As data on capsaicin-induced genotoxicity are limited and the effects of capsaicin against human lung A549 and DU145 prostate cancer cells were not explored in detail, we were interested in determining whether capsaicin-associated genotoxicity may also provoke A549 and DU145 cell death. Capsaicin-induced decrease in metabolic activity and cell proliferation, and changes in the cell cycle were limited to high concentrations used (≥ 100 μM), whereas, at lower concentrations, capsaicin stimulated both DNA double strand breaks and micronuclei production. Capsaicin was unable to provoke apoptotic cell death when used up to 250 μM concentrations. Capsaicin induced oxidative stress, but was ineffective in provoking the dissipation of the mitochondrial inner transmembrane potential. A different magnitude of p53 binding protein 1 (53BP1) recruitment contributed to diverse capsaicin-induced genotoxic effects in DU145 and A549 cells. Capsaicin was also found to be a DNA hypermethylating agent in A549 cells. In summary, we have shown that genotoxic effects of capsaicin may contribute to limited susceptibility of DU145 and A549 cancer cells to apoptosis in vitro, which may question the usefulness of capsaicin-based anticancer therapy, at least in a case of lung and prostate cancer.

  15. microRNA-145 Mediates the Inhibitory Effect of Adipose Tissue-Derived Stromal Cells on Prostate Cancer.

    Science.gov (United States)

    Takahara, Kiyoshi; Ii, Masaaki; Inamoto, Teruo; Nakagawa, Takatoshi; Ibuki, Naokazu; Yoshikawa, Yuki; Tsujino, Takuya; Uchimoto, Taizo; Saito, Kenkichi; Takai, Tomoaki; Tanda, Naoki; Minami, Koichiro; Uehara, Hirofumi; Komura, Kazumasa; Hirano, Hajime; Nomi, Hayahito; Kiyama, Satoshi; Asahi, Michio; Azuma, Haruhito

    2016-09-01

    Adipose-derived stromal cell (ASC), known as one of the mesenchymal stem cells (MSCs), is a promising tool for regenerative medicine; however, the effect of ASCs on tumor growth has not been studied sufficiently. We investigated the hypothesis that ASCs have an inhibitory effect on metastatic tumor progression. To evaluate the in vitro inhibitory effect of ASCs on metastatic prostate cancer (PCa), direct coculture and indirect separate culture experiments with PC3M-luc2 cells and human ASCs were performed, and ASCs were administered to PC3M-luc2 cell-derived tumor-bearing nude mice for in vivo experiment. We also performed exosome microRNA (miRNA) array analysis to explore a mechanistic insight into the effect of ASCs on PCa cell proliferation/apoptosis. Both in vitro and in vivo experiments exhibited the inhibitory effect of ASCs on PC3M-luc2 cell proliferation, inducing apoptosis and PCa growth, respectively. Among upregulated miRNAs in ASCs compared with fibroblasts, we focused on miR-145, which was known as a tumor suppressor. ASC-derived conditioned medium (CM) significantly inhibited PC3M-luc2 cell proliferation, inducing apoptosis, but the effect was canceled by miR-145 knockdown in ASCs. ASC miR-145 knockdown CM also reduced the expression of Caspase 3/7 with increased antiapoptotic protein, BclxL, expression in PC3M-luc2 cells. This study provides preclinical data that ASCs inhibit PCa growth, inducing PCa cell apoptosis with reduced activity of BclxL, at least in part, by miR-145, including exosomes released from ASCs, suggesting that ASC administration could be a novel and promising therapeutic strategy in patients with PCa.

  16. Corrective Action Investigation Plan for Corrective Action Unit 145: Wells and Storage Holes, Nevada Test Site, Nevada, Rev. No.: 0

    Energy Technology Data Exchange (ETDEWEB)

    David A. Strand

    2004-09-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information for conducting site investigation activities at Corrective Action Unit (CAU) 145: Wells and Storage Holes. Information presented in this CAIP includes facility descriptions, environmental sample collection objectives, and criteria for the selection and evaluation of environmental samples. Corrective Action Unit 145 is located in Area 3 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 145 is comprised of the six Corrective Action Sites (CASs) listed below: (1) 03-20-01, Core Storage Holes; (2) 03-20-02, Decon Pad and Sump; (3) 03-20-04, Injection Wells; (4) 03-20-08, Injection Well; (5) 03-25-01, Oil Spills; and (6) 03-99-13, Drain and Injection Well. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation (CAI) prior to evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. One conceptual site model with three release scenario components was developed for the six CASs to address all releases associated with the site. The sites will be investigated based on data quality objectives (DQOs) developed on June 24, 2004, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and Bechtel Nevada. The DQOs process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 145.

  17. Efficacy of Pseudomonas fluorescens (Pf-CL145A) spray dried powder for controlling zebra mussels adhering to test substrates

    Science.gov (United States)

    Luoma, James A.; Severson, Todd J.; Weber, Kerry L.; Mayer, Denise A.

    2015-01-01

    A mobile bioassay trailer was used to assess the efficacy of Pseudomonas fluorescens (Pf-CL145A) spray dried powder (SDP) formulation for controlling zebra mussels (Dreissena polymorpha) from two midwestern lakes: Lake Carlos (Alexandria, Minnesota) and Shawano Lake (Shawano, Wisconsin). The effects of SDP exposure concentration and exposure duration on zebra mussel survival were evaluated along with the evaluation of a benthic injection application technique to reduce the amount of SDP required to induce zebra mortality.

  18. Proteomic alteration of Marc-145 cells and PAMs after infection by porcine reproductive and respiratory syndrome virus.

    Science.gov (United States)

    Ding, Zhuang; Li, Zhi-jie; Zhang, Xiao-dong; Li, Ya-gang; Liu, Chang-jun; Zhang, Yan-Ping; Li, Yang

    2012-01-15

    Viral infections usually result in alterations in the host cell proteome, which determine the fate of infected cells and the progress of pathogenesis. To uncover cellular protein responses in porcine reproductive and respiratory syndrome virus (PRRSV), infected pulmonary alveolar macrophages (PAMs) and Marc-145 cells were subjected to proteomic analysis involving two-dimensional electrophoresis (2-DE) followed by MALDI-TOF-MS/MS identification. Altered expression of 44 protein spots in infected cells was identified in 2D gels, of which the 29 characterised by MALDI-TOF-MS/MS included 17 up-regulated and 12 down-regulated proteins. Some of these proteins were further confirmed at the mRNA level using real-time RT-PCR. Moreover, Western blot analysis confirmed the up-regulation of HSP27, vimentin and the down-regulation of galectin-1. Our study is the first attempt to analyze the cellular protein profile of PRRSV-infected Marc-145 cells using proteomics to provide valuable information about the effects of PRRSV-induced alterations on Marc-145 cell function. Further study of the affected proteins may facilitate our understanding of the mechanisms of PRRSV infection and pathogenesis.

  19. Regulation of DU145 prostate cancer cell growth by Scm-like with four mbt domains 2

    Indian Academy of Sciences (India)

    Kwanghyun Lee; Wonho Na; Je-Heon Maeng; Hongjin Wu; Bong-Gun Ju

    2013-03-01

    Mammalian SFMBTs have been considered to be polycomb group repressors. However, molecular mechanisms underlying mammalian SFMBTs-mediated gene regulation and their biological function have not been characterized. In the present study, we identified YY1 and methylated histones as interacting proteins of human SFMBT2. We also found that human SFMBT2 binds preferentially to methylated histone H3 and H4 that are associated with transcriptional repression. Using DU145 prostate cancer cells as a model, we showed that SFMBT2 has a transcriptional repression activity on HOXB13 gene expression. In addition, occupancy of SFMBT2 coincided with enrichment of diand tri-methylated H3K9 and H4K20 as well as tri-methylated H3K27 at the HOXB13 gene promoter. When SFMBT2 was depleted by siRNA in DU145 prostate cancer cells, significant up-regulation of HOXB13 gene expression and decreased cell growth were observed. Collectively, our findings indicate that human SFMBT2 may regulate cell growth via epigenetic regulation of HOXB13 gene expression in DU145 prostate cancer cells.

  20. Regulation of DU145 prostate cancer cell growth by Scm-like with four mbt domains 2.

    Science.gov (United States)

    Lee, Kwanghyun; Na, Wonho; Maeng, Je-Heon; Wu, Hongjin; Ju, Bong-Gun

    2013-03-01

    Mammalian SFMBTs have been considered to be polycomb group repressors. However, molecular mechanisms underlying mammalian SFMBTs-mediated gene regulation and their biological function have not been characterized. In the present study, we identified YY1 and methylated histones as interacting proteins of human SFMBT2. We also found that human SFMBT2 binds preferentially to methylated histone H3 and H4 that are associated with transcriptional repression. Using DU145 prostate cancer cells as a model, we showed that SFMBT2 has a transcriptional repression activity on HOXB13 gene expression. In addition, occupancy of SFMBT2 coincided with enrichment of diand tri-methylated H3K9 and H4K20 as well as tri-methylated H3K27 at the HOXB13 gene promoter. When SFMBT2 was depleted by siRNA in DU145 prostate cancer cells, significant up-regulation of HOXB13 gene expression and decreased cell growth were observed. Collectively, our findings indicate that human SFMBT2 may regulate cell growth via epigenetic regulation of HOXB13 gene expression in DU145 prostate cancer cells.

  1. A partner monoclonal antibody to Moab 730 kills 100% of DU145 and PC3 androgen-independent cancer cells

    Indian Academy of Sciences (India)

    Hemant Kumar Vyas; Rahul Pal; Nirmal K Lohiya; G P Talwar

    2009-12-01

    A number of therapeutic options are available for patients with prostate carcinoma till the time that the tumour is hormone dependent. However, no fully effective therapy is available for the treatment of androgen-independent prostate carcinomas. Antibodies directed at epitopes unique to or overexpressed on the cancer cells could be of therapeutic utility. A monoclonal antibody (Moab) 2C4 has been generated, which binds with cells of two androgenindependent prostate cancers, DU145 and PC3, and does not bind to peripheral blood leukocytes (PBLs) of healthy donors. This antibody, along with the previously developed Moab 730, kills 100% of both DU145 and PC3 cells in the presence of complement and does not have a deleterious effect on PBLs of healthy males. The anti-tumour action of the two antibodies prevents the establishment of DU145 cell tumour in nude mice in vivo. Moab 2C4 in combination with 730 has potential for use as therapy for androgen-independent cancers.

  2. Role of the inositol 1,4,5-trisphosphate receptor/Ca2+-release channel in autophagy

    Directory of Open Access Journals (Sweden)

    Parys Jan B

    2012-07-01

    Full Text Available Abstract Autophagy is an important cell-biological process responsible for the disposal of long-lived proteins, protein aggregates, defective organelles and intracellular pathogens. It is activated in response to cellular stress and plays a role in development, cell differentiation, and ageing. Moreover, it has been shown to be involved in different pathologies, including cancer and neurodegenerative diseases. It is a long standing issue whether and how the Ca2+ ion is involved in its regulation. The role of the inositol 1,4,5-trisphosphate receptor, the main intracellular Ca2+-release channel, in apoptosis is well recognized, but its role in autophagy only recently emerged and is therefore much less well understood. Positive as well as negative effects on autophagy have been reported for both the inositol 1,4,5-trisphosphate receptor and Ca2+. This review will critically present the evidence for a role of the inositol 1,4,5-trisphosphate receptor/Ca2+-release channel in autophagy and will demonstrate that depending on the cellular conditions it can either suppress or promote autophagy. Suppression occurs through Ca2+ signals directed to the mitochondria, fueling ATP production and decreasing AMP-activated kinase activity. In contrast, Ca2+-induced autophagy can be mediated by several pathways including calmodulin-dependent kinase kinase β, calmodulin-dependent kinase I, protein kinase C θ, and/or extracellular signal-regulated kinase.

  3. Chitosan polyplex nanoparticle vector for miR-145 expression in MCF-7: Optimization by design of experiment.

    Science.gov (United States)

    Tekie, Farnaz Sadat Mirzazadeh; Atyabi, Fatemeh; Soleimani, Masoud; Arefian, Ehsan; Atashi, Amir; Kiani, Melika; Khoshayand, Mohammad Reza; Amini, Mohsen; Dinarvand, Rassoul

    2015-11-01

    miR-145, a tumor suppressor micro RNA (miRNA), is down regulated in cancer and can be introduced as a therapeutic agent in various cancers including breast cancer. In this study, miR-145 plasmid was transfected to MCF-7 cells using chitosan polyplex nanoparticles. The vector was prepared according to an optimized fabricating method determined by response surface analysis and D-optimal design. Effects of chitosan molecular weight (Mw) and polymer amine to DNA phosphate ratio (N/P) as the variables were investigated on size, zeta potential, stability, and transfection efficiency of the polyplex nanoparticles. The results indicated that there is an interaction between effects of Mw and N/P ratio on the size of nanoparticles. Gel retardation assay demonstrated that the stability of the complexes in serum and preparation medium during storage time depends on the formulation variables. Statistical analysis affirmed that in spite of particle size, the variables of N/P ratio, time of incubation, and zeta potential affect the gene transfection. In conclusion, by selecting the perfect formulation prepared through an optimized method, it is possible to achieve a high transfection efficacy for miR-145 as an anticancer biological macromolecule.

  4. Five Year Outcome of 145 Patients With Ductal Carcinoma In Situ (DCIS) After Accelerated Breast Radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Ciervide, Raquel [Department of Radiation Oncology, New York University School of Medicine, NYU Langone Medical Center, New York, New York (United States); Dhage, Shubhada; Guth, Amber; Shapiro, Richard L.; Axelrod, Deborah M.; Roses, Daniel F. [Department of Surgery, New York University School of Medicine, NYU Langone Medical Center, New York, New York (United States); Formenti, Silvia C., E-mail: silvia.formenti@nyumc.org [Department of Radiation Oncology, New York University School of Medicine, NYU Langone Medical Center, New York, New York (United States)

    2012-06-01

    Background: Accelerated whole-breast radiotherapy (RT) with tumor bed boost in the treatment of early invasive breast cancer has demonstrated equivalent local control and cosmesis when compared with standard RT. Its efficacy in the treatment of ductal carcinoma in situ (DCIS) remains unknown. Methods and Materials: Patients treated for DCIS with lumpectomy and negative margins were eligible for 2 consecutive hypofractionated whole-breast RT clinical trials. The first trial (New York University [NYU] 01-51) prescribed to the whole breast 42 Gy (2.8 Gy in 15 fractions) and the second trial (NYU 05-181) 40.5 Gy (2.7 Gy in 15 fractions) with an additional daily boost of 0.5 Gy to the surgical cavity. Results: Between 2002 and 2009, 145 DCIS patients accrued, 59 to the first protocol and 86 to the second trial. Median age was 56 years and 65% were postmenopausal at the time of treatment. Based on optimal sparing of normal tissue, 79% of the patients were planned and treated prone and 21% supine. At 5 years' median follow-up (60 months; range 2.6-105.5 months), 6 patients (4.1%) experienced an ipsilateral breast recurrence in all cases of DCIS histology. In 3/6 patients, recurrence occurred at the original site of DCIS and in the remaining 3 cases outside the original tumor bed. New contralateral breast cancers arose in 3 cases (1 DCIS and 2 invasive carcinomas). Cosmetic self-assessment at least 2 years after treatment is available in 125 patients: 91% reported good-to-excellent and 9% reported fair-to-poor outcomes. Conclusions: With a median follow-up of 5 years, the ipsilateral local recurrence rate is 4.1%, comparable to that reported from the NSABP (National Surgical Adjuvant Breast and Bowel Project) trials that employed 50 Gy in 25 fractions of radiotherapy for DCIS. There were no invasive recurrences. These results provide preliminary evidence that accelerated hypofractionated external beam radiotherapy is a viable option for DCIS.

  5. Inositol (1,4,5)-trisphosphate activates a calcium channel in isolated sarcoplasmic reticulum membranes.

    Science.gov (United States)

    Suárez-Isla, B. A.; Irribarra, V.; Oberhauser, A.; Larralde, L.; Bull, R.; Hidalgo, C.; Jaimovich, E.

    1988-01-01

    Sarcoplasmic reticulum membrane vesicles isolated from frog skeletal muscle display high conductance calcium channels when fused into phospholipid bilayers. The channels are selective for calcium and barium over Tris. The fractional open time was voltage-independent (-40 to +25 mV), but was steeply dependent on the free cis [Ca2+] (P0 = 0.02 at 10 microM cis Ca2+ and 0.77 at 150 microM Ca2+; estimated Hill coefficient: 1.6). Addition of ATP (1 mM; cis) further increased P0 from 0.77 to 0.94. Calcium activation was reversed by addition of EGTA to the cis compartment. Magnesium (2 mM) increased the frequency of rapid closures and 8 mM magnesium decreased the current amplitude from 3.4 to 1.2 pA at 0 mV, suggesting a reversible fast blockade. Addition of increasing concentrations of inositol (1, 4, 5)-triphosphate (cis), increased P0 from 0.10 +/- 0.01 (mean +/- SEM) in the control to 0.85 +/- 0.02 at 50 microM in an approximately sigmoidal fashion, with an apparent half-maximal activation at 15 microM inositol (1, 4, 5)-trisphosphate in the presence of 40 microM cis Ca2+. Lower concentrations of this agonist were required to produce a significant increase in P0 when 10 microM or less cis Ca2+ were used. The channel was blocked by the addition to the cis compartment of either 0.5 mM lanthanum, 0.5 microM ruthenium red, or 200 nM ryanodine, all known inhibitors of Ca2+ release from sarcoplasmic reticulum vesicles. These results demonstrate the presence of calcium channels in the sarcoplasmic reticulum from frog skeletal muscle with a pharmacological profile consistent with a role in excitation contraction coupling and with the hypothesis that inositol ( 1,4,5)-trisphosphate is a physiological agonist in this process. PMID:2852037

  6. GPNMB/OA protein increases the invasiveness of human metastatic prostate cancer cell lines DU145 and PC3 through MMP-2 and MMP-9 activity

    Energy Technology Data Exchange (ETDEWEB)

    Fiorentini, Chiara; Bodei, Serena; Bedussi, Francesca; Fragni, Martina; Bonini, Sara Anna [Section of Pharmacology, Department of Molecular and Translational Medicine, University of Brescia, V.le Europa 11, 25124 Brescia (Italy); Simeone, Claudio; Zani, Danilo [Division of Urology, Department of Surgery, Radiology and Public Health, University of Brescia, P.le Spedali Civili 1, 25124 Brescia (Italy); Berruti, Alfredo [Medical Oncology, Department of Surgery, Radiology, and Public Health, University of Brescia, P.le Spedali Civili 1, 25124 Brescia (Italy); Missale, Cristina; Memo, Maurizio; Spano, PierFranco [Section of Pharmacology, Department of Molecular and Translational Medicine, University of Brescia, V.le Europa 11, 25124 Brescia (Italy); Sigala, Sandra, E-mail: sigala@med.unibs.it [Section of Pharmacology, Department of Molecular and Translational Medicine, University of Brescia, V.le Europa 11, 25124 Brescia (Italy)

    2014-04-15

    Non-metastatic glycoprotein melanoma protein B (GPNMB), also known as osteoactivin (OA) is expressed in a wide array of tumors and represents an emerging target for drug development. In this study, we investigated the role of GPNMB/OA in the progression of human metastatic DU145 and PC3 prostate cancer cells. GPNMB/OA contribution in PCa malignant phenotype has been analyzed by small interfering RNA-induced GPNMB/OA silencing. We found that following GPNMB/OA silencing the migration capability of both DU145 and PC3 cells, evaluated by using in vitro invasivity assay, as well as the metalloproteinases MMP-2 and MMP-9 activity were equally strongly inhibited. By contrast knocking down GPNMB/OA weakly attenuated cell proliferation rate of DU145, an effect that paralleled with an increase number of apoptotic cells. However, PC3 cell growth seems to be not affected by GPNMB/OA. Together, these data reveal that GPNMB/OA acts as a critical molecular mediator promoting the acquisition of the more aggressive, pro-metastatic phenotype distinctive of human DU145 and PC3 cell lines. - Highlights: • GPNMB/OA expression correlates with DU145 and PC3 cells malignant phenotype. • GPNMB/OA silencing affects the migration capability of both DU145 and PC3 cells. • GPNMB/OA increases invasiveness by up-regulating MMPs activity. • GPNMB/OA promotes DU145 and PC3 cells progression into a more aggressive phenotype.

  7. Enterovirus 71 binding to PSGL-1 on leukocytes: VP1-145 acts as a molecular switch to control receptor interaction.

    Directory of Open Access Journals (Sweden)

    Yorihiro Nishimura

    Full Text Available Some strains of enterovirus 71 (EV71, but not others, infect leukocytes by binding to a specific receptor molecule: the P-selectin glycoprotein ligand-1 (PSGL-1. We find that a single amino acid residue within the capsid protein VP1 determines whether EV71 binds to PSGL-1. Examination of capsid sequences of representative EV71 strains revealed that the PSGL-1-binding viruses had either a G or a Q at residue 145 within the capsid protein VP1 (VP1-145G or Q, whereas PSGL-1-nonbinding viruses had VP1-145E. Using site-directed mutagenesis we found that PSGL-1-binding strains lost their capacity to bind when VP1-145G/Q was replaced by E; conversely, nonbinding strains gained the capacity to bind PSGL-1 when VP1-145E was replaced with either G or Q. Viruses with G/Q at VP1-145 productively infected a leukocyte cell line, Jurkat T-cells, whereas viruses with E at this position did not. We previously reported that EV71 binds to the N-terminal region of PSGL-1, and that binding depends on sulfated tyrosine residues within this region. We speculated that binding depends on interaction between negatively charged sulfate groups and positively charged basic residues in the virus capsid. VP1-145 on the virus surface is in close proximity to conserved lysine residues at VP1-242 and VP1-244. Comparison of recently published crystal structures of EV71 isolates with either Q or E at VP1-145 revealed that VP1-145 controls the orientation of the lysine side-chain of VP1-244: with VP1-145Q the lysine side chain faces outward, but with VP1-145E, the lysine side chain is turned toward the virus surface. Mutation of VP1-244 abolished virus binding to PSGL-1, and mutation of VP1-242 greatly reduced binding. We propose that conserved lysine residues on the virus surface are responsible for interaction with sulfated tyrosine residues at the PSGL-1 N-terminus, and that VP1-145 acts as a switch, controlling PSGL-1 binding by modulating the exposure of VP1-244K.

  8. Enterovirus 71 binding to PSGL-1 on leukocytes: VP1-145 acts as a molecular switch to control receptor interaction.

    Science.gov (United States)

    Nishimura, Yorihiro; Lee, Hyunwook; Hafenstein, Susan; Kataoka, Chikako; Wakita, Takaji; Bergelson, Jeffrey M; Shimizu, Hiroyuki

    2013-01-01

    Some strains of enterovirus 71 (EV71), but not others, infect leukocytes by binding to a specific receptor molecule: the P-selectin glycoprotein ligand-1 (PSGL-1). We find that a single amino acid residue within the capsid protein VP1 determines whether EV71 binds to PSGL-1. Examination of capsid sequences of representative EV71 strains revealed that the PSGL-1-binding viruses had either a G or a Q at residue 145 within the capsid protein VP1 (VP1-145G or Q), whereas PSGL-1-nonbinding viruses had VP1-145E. Using site-directed mutagenesis we found that PSGL-1-binding strains lost their capacity to bind when VP1-145G/Q was replaced by E; conversely, nonbinding strains gained the capacity to bind PSGL-1 when VP1-145E was replaced with either G or Q. Viruses with G/Q at VP1-145 productively infected a leukocyte cell line, Jurkat T-cells, whereas viruses with E at this position did not. We previously reported that EV71 binds to the N-terminal region of PSGL-1, and that binding depends on sulfated tyrosine residues within this region. We speculated that binding depends on interaction between negatively charged sulfate groups and positively charged basic residues in the virus capsid. VP1-145 on the virus surface is in close proximity to conserved lysine residues at VP1-242 and VP1-244. Comparison of recently published crystal structures of EV71 isolates with either Q or E at VP1-145 revealed that VP1-145 controls the orientation of the lysine side-chain of VP1-244: with VP1-145Q the lysine side chain faces outward, but with VP1-145E, the lysine side chain is turned toward the virus surface. Mutation of VP1-244 abolished virus binding to PSGL-1, and mutation of VP1-242 greatly reduced binding. We propose that conserved lysine residues on the virus surface are responsible for interaction with sulfated tyrosine residues at the PSGL-1 N-terminus, and that VP1-145 acts as a switch, controlling PSGL-1 binding by modulating the exposure of VP1-244K.

  9. socs7, a target gene of microRNA-145, regulates interferon-β induction through STAT3 nuclear translocation in bladder cancer cells.

    Science.gov (United States)

    Noguchi, S; Yamada, N; Kumazaki, M; Yasui, Y; Iwasaki, J; Naito, S; Akao, Y

    2013-02-07

    We recently reported that microRNA (miR)-145 is downregulated and induces apoptosis in human bladder cancer cells. Also, it is suggested that the ectopic expression of miR-145 induces apoptosis with the induction of TRAIL expression in several cancer cells. Here, we demonstrated a novel mechanism of apoptosis induction by miR-145 in bladder cancer cells. Exogenous miR-145 in T24 and NKB1 cells markedly increased the expression levels of interferon (IFN)-β, 2'-5'-oligoadenylate synthetase 1, which lies upstream of 2'-5' oligoadenylates/RNase L system, and TRAIL, and induced apparent caspase-dependent apoptosis that was suppressed by cotreatment with a pan-caspase inhibitor; moreover, these expression levels were reduced by cotreatment with an miR-145 inhibitor. The apoptosis did not depend on Toll-like receptor 3 (TLR3) expression, because TLR3-silencing failed to inhibit IFN-β induction by miR-145. Then, we focused on the suppressor of cytokine signaling 7 (socs7), whose expression level was upregulated in bladder cancer cells compared with its level in normal human urothelial cells, as a putative target gene involved in IFN-β induction by miR-145. Expectedly, exogenous miR-145 decreased the expression level of SOCS7, and socs7-silencing enhanced IFN-β induction by transfection with a TLR3 ligand, polyinosinic acid-polycytidylic acid (PIC). The results of a luciferase reporter assay revealed that miR-145 targeted socs7. In addition, socs7-silencing significantly decreased the level of p-Akt and suppressed the growth of T24 cells. Furthermore, exogenous miR-145 or socs7-silencing promoted nuclear translocation of STAT3. In conclusion, the machinery of IFN-β induction through the regulation of SOCS7 by miR-145 was closely associated with the induction of apoptosis. Moreover, exogenous miR-145 promoted IFN-β induction by targeting socs7, which resulted in the nuclear translocation of STAT3. Additionally, our data indicate that SOCS7 functioned as an oncogene

  10. 人前列腺癌细胞系DU145中肿瘤干细胞样侧群细胞的分离%Identification of cancer stem cell -like side population cells in human prostate cancer cell line DU 145

    Institute of Scientific and Technical Information of China (English)

    殷波; 刘岗; 张辉; 李明; 隋文印; 宋永胜

    2012-01-01

    Objective;To identify the side population (SP) cells from human prostate cancer cell line DU 145 and characterize DU 145 SP cells preliminary. Methods: Fluorescence activated cell sorting (FACS) was applied to isolate SP cells from DU 145 cell line. The isolated SP cells were cultured in serum - free medium to observe the growth pattern. Reverse transcription - polymerase chain reaction (RT - PCR) was used to detect the expression of ABCG2 in DU 145 SP cells and parental DU 145 cells. Results: SP cells were isolated from DU 145 cells by flow cytometry, with a proportion of 1.1%. When cultured in serum -free medium, SP cells showed a clustered growth pattern compared to the parental DU 145 cells. Furthermore, a higher expression of ABCG2 in SP cells was detected by RT -PCR in contrast to parental DU 145 cells. Conclusion; DU 145 cell line contained SP cells with cancer stem cell properties.%目的:分离人前列腺癌细胞系DU 145中的侧群(side population, SP)细胞,并初步分析其生物学特性.方法:采用荧光激活细胞分类(fluorescence activated cell sorting,FACS)技术,从DU 145细胞中分离出侧群细胞,并检测其比例;继而培养于无血清培养基中,观察其生长特性.采用反转录聚合酶链反应( RT-PCR)技术检测侧群细胞中ABCG2的表达水平.结果:DU 145细胞中存在含量极少的侧群细胞,比例约1.1%;培养于无血清培养基中成簇生长.和对应的母系DU 145细胞相比,DU 145侧群细胞的ABCG2表达增高.结论:人前列腺癌细胞系DU 145中存在具有肿瘤干细胞特性的侧群细胞.

  11. A receptor tyrosine kinase, UFO/Axl, and other genes isolated by a modified differential display PCR are overexpressed in metastatic prostatic carcinoma cell line DU145.

    Science.gov (United States)

    Jacob, A N; Kalapurakal, J; Davidson, W R; Kandpal, G; Dunson, N; Prashar, Y; Kandpal, R P

    1999-01-01

    We have used a modified differential display PCR protocol for isolating 3' restriction fragments of cDNAs specifically expressed or overexpressed in metastatic prostate carcinoma cell line DU145. Several cDNA fragments were identified that matched to milk fat globule protein, UFO/Axl, a receptor tyrosine kinase, human homologue of a Xenopus maternal transcript, laminin and laminin receptor, human carcinoma-associated antigen, and some expressed sequence tags. The transcript for milk fat globule protein, a marker protein shown to be overexpressed in breast tumors, was elevated in DU145 cells. The expression of UFO/Axl, a receptor tyrosine kinase, was considerably higher in DU145 cells as compared to normal prostate cells and prostatic carcinoma cell line PC-3. The overexpression of UFO oncogene in DU145 cells is discussed in the context of prostate cancer metastasis.

  12. Ins(1,4,5)P3 interacts with PIP2 to regulate activation of TRPC6/C7 channels by diacylglycerol in native vascular myocytes.

    Science.gov (United States)

    Ju, Min; Shi, Jian; Saleh, Sohag N; Albert, Anthony P; Large, William A

    2010-05-01

    We investigated synergism between inositol 1,4,5-trisphosphate (Ins(1,4,5)P(3)) and diacylglycerol (DAG) on TRPC6-like channel activity in rabbit portal vein myocytes using single channel recording and immunoprecipitation techniques. Ins(1,4,5)P(3) at 10 microm increased 3-fold TRPC6-like activity induced by 10 microm 1-oleoyl-2-acetyl-sn-glycerol (OAG), a DAG analogue. Ins(1,4,5)P(3) had no effect on OAG-induced TRPC6 activity in mesenteric artery myocytes. Anti-TRPC6 and anti-TRPC7 antibodies blocked channel activity in portal vein but only anti-TRPC6 inhibited activity in mesenteric artery. TRPC6 and TRPC7 proteins strongly associated in portal vein but only weakly associated in mesenteric artery tissue lysates. Therefore in portal vein the conductance consists of TRPC6/C7 subunits, while OAG activates a homomeric TRPC6 channel in mesenteric artery myocytes. Wortmannin at 20 microm reduced phosphatidylinositol 4,5-bisphosphate (PIP(2)) association with TRPC6 and TRPC7, and produced a 40-fold increase in OAG-induced TRPC6/C7 activity. Anti-PIP(2) antibodies evoked TRPC6/C7 activity, which was blocked by U73122, a phospholipase C inhibitor. DiC8-PIP(2), a water-soluble PIP(2) analogue, inhibited OAG-induced TRPC6/C7 activity with an IC(50) of 0.74 microm. Ins(1,4,5)P(3) rescued OAG-induced TRPC6/C7 activity from inhibition by diC8-PIP(2) in portal vein myocytes, and this was not prevented by the Ins(1,4,5)P(3) receptor antagonist heparin. In contrast, Ins(1,4,5)P(3) did not overcome diC8-PIP(2)-induced inhibition of TRPC6 activity in mesenteric artery myocytes. 2,3,6-Tri-O-butyryl-Ins(1,4,5)P(3)/AM (6-Ins(1,4,5)P(3)), a cell-permeant analogue of Ins(1,4,5)P(3), at 10 microm increased TRPC6/C7 activity in portal vein and reduced association between TRPC7 and PIP(2), but not TRPC6 and PIP(2). In contrast, 10 microm OAG reduced association between TRPC6 and PIP(2), but not between TRPC7 and PIP(2). The present work provides the first evidence that Ins(1,4,5)P(3

  13. Organization of Ca2+ stores in myeloid cells: association of SERCA2b and the type-1 inositol-1,4,5-trisphosphate receptor.

    Science.gov (United States)

    Favre, C J; Jerström, P; Foti, M; Stendhal, O; Huggler, E; Lew, D P; Krause, K H

    1996-05-15

    In this study, we have analysed the relationship between Ca2+ pumps and Ins(1,4,5)P3-sensitive Ca2+ channels in myeloid cells. To study whether sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase (SERCA)-type Ca(2+)-ATPases are responsible for Ca2+ uptake into Ins(1,4,5)P3-sensitive Ca2+ stores, we used the three structurally unrelated inhibitors thapsigargin, 2,5-di-t-butylhydroquinone and cyclopiazonic acid. In HL-60 cells, all three compounds precluded formation of the phosphorylated intermediate of SERCA-type Ca(2+)-ATPases. They also decreased, in parallel, ATP-dependent Ca2+ accumulation and the amount of Ins(1,4,5)P3-releasable Ca2+. Immunoblotting with subtype-directed antibodies demonstrated that HL-60 cells contain the Ca2+ pump SERCA2 (subtype b), and the Ca(2+)-release-channel type-1 Ins(1,4,5)P3 receptor. In subcellular fractionation studies, SERCA2 and type-1 Ins(1,4,5)P3 receptor co-purified. Immunofluorescence studies demonstrated that both type-1 Ins(1,4,5)P3 receptor and SERCA2 were evenly distributed throughout the cell in moving neutrophils. During phagocytosis both proteins translocated to the periphagosomal space. Taken together, our results suggest that in myeloid cells (i) SERCA-type Ca(2+)-ATPases function as Ca2+ pumps of Ins(1,4,5)P3-sensitive Ca2+ stores, and (ii) SERCA2 and type-1 Ins(1,4,5)P3 receptor reside either in the same or two tightly associated subcellular compartments.

  14. Caenorhabditis elegans inositol 5-phosphatase homolog negatively regulates inositol 1,4,5-triphosphate signaling in ovulation.

    Science.gov (United States)

    Bui, Yen Kim; Sternberg, Paul W

    2002-05-01

    Ovulation in Caenorhabditis elegans requires inositol 1,4,5-triphosphate (IP(3)) signaling activated by the epidermal growth factor (EGF)-receptor homolog LET-23. We generated a deletion mutant of a type I 5-phosphatase, ipp-5, and found a novel ovulation phenotype whereby the spermatheca hyperextends to engulf two oocytes per ovulation cycle. The temporal and spatial expression of IPP-5 is consistent with its proposed inhibition of IP(3) signaling in the adult spermatheca. ipp-5 acts downstream of let-23, and interacts with let-23-mediated IP(3) signaling pathway genes. We infer that IPP-5 negatively regulates IP(3) signaling to ensure proper spermathecal contraction.

  15. MicroRNA-145 Inhibits Cell Migration and Invasion and Regulates Epithelial-Mesenchymal Transition (EMT) by Targeting Connective Tissue Growth Factor (CTGF) in Esophageal Squamous Cell Carcinoma.

    Science.gov (United States)

    Han, Qiang; Zhang, Hua-Yong; Zhong, Bei-Long; Wang, Xiao-Jing; Zhang, Bing; Chen, Hua

    2016-10-23

    BACKGROUND This study investigated the mechanism of miR-145 in targeting connective tissue growth factor (CTGF), which affects the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of ESCC cells. MATERIAL AND METHODS A total of 50 ESCC tissues and their corresponding normal adjacent esophageal tissue samples were collected. Then, miR-145 expression in both ESCC clinical specimens and cell lines was detected using quantitative real-time PCR. CTGF protein was detected using immunohistochemistry. Dual luciferase reporter gene assay was employed to assess the effect of miR-145 on the 3'UTR luciferase activity of CTGF. Eca109 cells were transfected with miR-145 mimics and CTGF siRNA, respectively, and changes in cellular proliferation, migration, and invasion were detected via MTT assay, wound-healing assay, and Transwell assay, respectively. Western blotting assay was used to detect the expression of marker genes related to EMT. RESULTS MiR-145 was significantly down-regulated in ESCC tissues and cell lines compared with normal tissues and cell lines (Ptissues was than in normal adjacent esophageal tissues (Ptissues and cell lines, while the protein expression of CTGF exhibited the opposite trend. MiR-145 inhibited the proliferation, migration, invasiveness, and the EMT process of ESCC cells through targeted regulation of CTGF expression.

  16. The expression of miR-143 and miR-145 in human gastric cancer and its clinical significance%miR-143和miR-145在胃癌中的表达和临床意义

    Institute of Scientific and Technical Information of China (English)

    孙兴邦; 张玄; 赵晶; 高超

    2015-01-01

    Objective To investigate the expression of miR-143 and miR-145 in human gastric cancer and its re-lationship with pathological characteristics.Methods The amounts of miR-143 and miR-145 in gastric cancer were detected by real-time PCR.Meanwhile, their association with clinical stages and grades were analyzed.Results Ac-cording to real-time PCR, gastric cancer samples presented remarkably reduced levels of miR-143 and miR-145 than normal adjacent tissues.The expression of miR-143 and miR-145 in gastric cancer were correlated with TNM stage, tumor differentiation and lymph node metastasis, rather than to age and gender.The levels of miR-143 and miR-145 was positively correlated in gastric cancer (r=0.792, P<0.05).Conclusion The quantities of miR-143 and miR-145 were significantly decreased in gastric cancer, which may be closely related with the pathogenesis of gastric cancer.%目的:探讨胃癌组织中miR-143、miR-145的表达及其与胃癌临床病理特征的关系。方法采用Real-time PCR法检测miR-143、miR-145在胃癌组织中的表达,分析miR-143和miR-145的表达与胃癌临床分期、分级的关系。结果 Real-time PCR法检测提示miR-143和miR-145在胃癌组织中较癌旁组织中表达明显降低,差异具有统计学意义(P<0.05)。 miR-143和miR-145的低表达与临床分期、有无淋巴结转移及分化程度有关,2种miR的表达与年龄、性别无关。胃癌组织中miR-143、miR-145表达呈正相关( r=0.792,P<0.05)。结论 miR-143和miR-145在胃癌中明显低表达,可能与胃癌的发生密切相关。

  17. MicroRNA 145 may play an important role in uveal melanoma cell growth by potentially targeting insulin receptor substrate-1

    Institute of Scientific and Technical Information of China (English)

    Li Yang; Huang Qiming; Shi Xuehui; Jin Xiang; Shen Li; Xu Xiaolin; Wei Wenbin

    2014-01-01

    Background MicroRNAs (miRNAs) contribute to tumorigenesis by acting as either oncogenes or tumor suppressor genes.In this study,we investigated the role of miR-145 in the pathogenesis of uveal melanoma.Methods Expression profiles of miRNAs in uveal melanoma were performed using Agilent miRNA array.Quantitative real-time polymerase chain reaction was used to screen the expression levels of miR-145 in normal uveal tissue,uveal melanoma tissue,and uveal melanoma cell lines.Lenti-virus expression system was used to construct MUM-2B and OCM-1 cell lines with stable overexpression of miR-145.Cell proliferation,cell cycle,and cell apoptosis of these miR-145 overexpression cell lines were examined by MTT assay and flow cytometry respectively.The target genes of miR-145 were predicted by bioinformatics and confirmed using a luciferase reporter assay.The expression of insulin-like growth factor-1 receptor (IGF-1R),insulin receptor substrate-1 (IRS-1) proteins was determined by Western blotting analysis.IRS-1 was knocked down in OCM-1 cells.TUNEL,BrdU,and flow cytometry assay were performed in IRS-1 knocked down OCM-1 cell lines to analyze its function.Results Forty-seven miRNAs were up regulated in uveal melanoma and 61 were down regulated.miR-145 expression was significantly lower in uveal melanoma sample and the cell lines were compared with normal uveal sample.Overexpression of miR-145 suppressed cell proliferation by blocking the G1 phase entering S phase in uveal melanoma cells,and promoted uveal melanoma cell apoptosis.IRS-1 was identified as a potential target of miR-145 by dual luciferase reporter assay.Knocking down of IRS-1 had similar effect as overexpression of miR-145.Conclusion miR-145 might act as a tumor suppressor in uveal melanoma,and downregulation of the target IRS-1 might be a potential mechanism.

  18. Artocarpus altilis (Parkinson) Fosberg Extracts and Geranyl Dihydrochalcone Inhibit STAT3 Activity in Prostate Cancer DU145 Cells.

    Science.gov (United States)

    Jeon, Yoon Jung; Jung, Seung-Nam; Chang, Hyeyoun; Yun, Jieun; Lee, Chang Woo; Lee, Joonku; Choi, Sangho; Nash, Oyekanmi; Han, Dong Cho; Kwon, Byoung-Mog

    2015-05-01

    Artocarpus altilis (Parkinson) Fosberg has traditionally been used in Indonesia for the treatment of liver cirrhosis, hypertension, and diabetes. In many other countries, it is used for the treatment of malaria, yellow fever, and dengue fever. It has been reported that A. altilis extracts have antiatherosclerotic and cytoprotective effects, but its molecular targets in tumor cells are not yet fully understood. The A. altilis extracts and the partially purified fraction have been shown to inhibit STAT3 activity and the phosphorylation of STAT3 in a dose-dependent manner. To identify the active components, a bioassay-guided isolation of the partially purified fraction resulted in the identification of a geranyl dihydrochalcone, CG901. Its chemical structure was established on the basis of spectroscopic evidence and comparison with published data. The partially purified fraction and the isolated a geranyl dihydrochalcone, CG901, down-regulated the expression of STAT3 target genes, induced apoptosis in DU145 prostate cancer cells via caspase-3 and PARP degradation, and inhibited tumor growth in human prostate tumor (DU145) xenograft initiation model. These results suggest that A. altilis could be a good natural source and that the isolated compound will be a potential lead molecule for developing novel therapeutics against STAT3-related diseases, including cancer and inflammation. Copyright © 2015 John Wiley & Sons, Ltd.

  19. Exploring the multiband emission of TXS 0536+145: the most distant gamma-ray flaring blazar

    CERN Document Server

    Orienti, M; Giroletti, M; Finke, J; Ajello, M; Dallacasa, D; Venturi, T

    2014-01-01

    We report results of a multiband monitoring campaign of the flat spectrum radio quasar TXS 0536+145 at redshift 2.69. This source was detected during a very high gamma-ray activity state in 2012 March by the Large Area Telescope on board Fermi, becoming the gamma-ray flaring blazar at the highest redshift detected so far. At the peak of the flare the source reached an apparent isotropic gamma-ray luminosity of 6.6 x 10^49 erg/s which is comparable to the values achieved by the most luminous blazars. This activity triggered radio-to-X-rays monitoring observations by Swift, Very Long Baseline Array, European VLBI Network, and Medicina single-dish telescope. Significant variability was observed from radio to X-rays supporting the identification of the gamma-ray source with TXS 0536+145. Both the radio and gamma-ray light curves show a similar behaviour, with the gamma-rays leading the radio variability with a time lag of about 4-6 months. The luminosity increase is associated with a flattening of the radio spect...

  20. Measurement of x-ray energy spectrum by using HPGe detection in 14.5 GHz ECR ion source

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Cheol Ho; Chang, Dae Sik; Oh, Byung Hoon [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Seo, Chang Seog; Kim, Yong Kyun [Institute for Basic Science, Daejeon (Korea, Republic of)

    2013-04-15

    The Electron Cyclotron Resonance (ECR) ion source is used to produce intense, high charge state ion beams of intermediate and heavy mass elements. It is widely used to produce ion beams for accelerator, atomic physics research and industrial application. The basic principle of the ECR ion source is the resonance absorption of energy by electron from microwave that has the same frequency as the electron's frequency in the resonance zone. The ECR ion source produces soft and hard x-rays because of efficient heating of electrons. The x-rays are created by electron-ion collisions in the ECR plasma or, when free electrons collide with ECR plasma chamber wall. The generated x-rays are influenced by various input parameters of the ECR ion source. In this study, The x-ray spectrum was measured by using a 14.5 GHz ECR ion source at Korea Atomic Energy Research Institute (KAERI). ECR ion source is used to generate ion beams of heavy mass elements. KAERI has a 14.5 GHz ECR ion source to produce high current ion beam. In this study, experimental condition is provided to generate stable plasma through x-ray spectrum measurement. In the future, x-rays spectra will be measured at various operation conditions such as gas-pressure, trim coil and solenoid current.

  1. 21 CFR 145.145 - Canned grapefruit.

    Science.gov (United States)

    2010-04-01

    ... as measured on the refractometer, expressed as percent by weight sucrose (degrees Brix) with... the next lower category or 2 percent by weight sucrose (degrees Brix) lower if no lower category... statement of substandard quality, “Good Food—Not High Grade”, a new line as specified after...

  2. Multivalent benzene polyphosphate derivatives are non-Ca(2+)-mobilizing Ins(1,4,5)P3 receptor antagonists.

    Science.gov (United States)

    Mills, Stephen J; Luyten, Tomas; Erneux, Christophe; Parys, Jan B; Potter, Barry V L

    2012-12-01

    Inositol 1,4,5-trisphosphate [Ins(1,4,5)P31] mobilizes intracellular Ca(2+) through the Ins(1,4,5)P3 receptor [InsP3R]. Although some progress has been made in the design of synthetic InsP3R partial agonists and antagonists, there are still few examples of useful small molecule competitive antagonists. A "multivalent" approach is explored and new dimeric polyphosphorylated aromatic derivatives were designed, synthesized and biologically evaluated. The established weak InsP3R ligand benzene 1,2,4-trisphosphate [Bz(1,2,4)P32] is dimerized through its 5-position in two different ways, first directly as the biphenyl derivative biphenyl 2,2',4,4',5,5'-hexakisphosphate, [BiPh(2,2',4,4',5,5')P68] and with its regioisomeric biphenyl 3,3',4,4',5,5'-hexakisphosphate [BiPh(3,3',4,4',5,5')P611]. Secondly, a linker motif is introduced in a flexible ethylene-bridged dimer (9) with its corresponding 1,2-bisphosphate dimer (10), both loosely analogous to the very weak antagonist 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA 7). In permeabilized L15 fibroblasts overexpressing type 1 InsP3R, BiPh(2,2',4,4',5,5')P6 (8) inhibits Ins(1,4,5)P3-induced Ca(2+) release in a apparently competitive fashion [IC50 187 nM] and the Bz(1,2,4)P3 dimer (9) is only slightly weaker [IC50 380 nM]. Compounds were also evaluated against type I Ins(1,4,5)P3 5-phosphatase. All compounds are resistant to dephosphorylation, with BiPh(2,2',4,4',5,5')P6 (8), being the most effective inhibitor of any biphenyl derivative synthesized to date [IC50 480 nM] and the Bz(1,2,4)P3 ethylene dimer (9) weaker [IC50 3.55 μM]. BiPh(3,3',4,4',5,5')P6 (11) also inhibits 5-phosphatase [IC50 730 nM] and exhibits unexpected Ca(2+) releasing activity [EC50 800 nM]. Thus, relocation of only a single mirrored phenyl phosphate group in (11) from that of antagonist (8) does not markedly change enzyme inhibitory activity, but elicits a dramatic switch in Ca(2+)-releasing activity. Such new agents demonstrate the

  3. Nd2Fe14.5Cr2.5化合物的结构和相变%Structure and Phase Transition of Nd2Fe14.5 Cr2.5 Compound

    Institute of Scientific and Technical Information of China (English)

    高纯静; 郝延明; 梁飞飞; 郑开萍; 胡怀古

    2012-01-01

    通过X射线衍射、差热分析及磁测量等手段对电弧炉熔炼的Nd2Fe14.5Cr2.5化合物的结构和相变进行了研究,研究结果表明,950℃下退火的样品具有Th2Zn17型结构,其居里温度为375 K.1100℃下退火的样品具有Nd3(Fe.Ti)29型结构,其居里温度为445 K.Nd2Fe14 5Cr2.5化合物在1100℃左右发生结构相变,山菱方相的Th2Zn17型结构转变成单斜相的Nd3(Fe,Ti)29型结构.具有Th2Zn17型相结构的Nd2Fe14.5Cr2.5化合物在约300℃左右可以发生有效的吸氮反应.%Structure and its phase transitions of the arc-melted Nd2Fe14.5 Cr2.5 were investigated by using differential thermal analyzer, X-ray diffractions, and magnetization measurements. The result showed that the sample annealed at 950 ℃. had a Th2Zn17-type structure, its Curie temperature was about 375 K. The sample annealed at 1100 ℃ Nd3(Fe,Ti)29 structure, its Curie temperature was about 445 K. At about 1100 ℃ , the structure phase transition was occurred, and the rhombohedral Th2Zn,7-type structure translated into monoclinic Nd3 ( Fe, Ti) 29-type structure. The nitrogenized reaction of the compound with Th2Zn,7-type structure was more sufficient at about 300℃. The detailed discussion was presented.

  4. Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and the corresponding mechanism.

    Science.gov (United States)

    Shi, Runlin; Xiao, Haibing; Yang, Tao; Chang, Lei; Tian, Yuanfeng; Wu, Bolin; Xu, Hua

    2014-12-01

    microRNAs (miRNAs) have played a key role in human tumorigenesis, tumor progression, and metastasis. On the one hand, miRNAs are aberrantly expressed in many types of human cancer; on the other hand, miRNAs can function as tumor suppressors or oncogenes that target many cancer-related genes. This study aimed to investigate the effects of miRNA-200c (miR-200c) on the biological behavior and mechanism of proliferation, migration, and invasion in the prostate cancer cell line Du145. In this study, Du145 cells were transfected with miR-200c mimics or negative control miR-NC by using an X-tremeGENE siRNA transfection reagent. The relative expression of miR-200c was measured by RT-PCR. The proliferation, migration, and invasion abilities of Du145 cells were detected by CCK8 assays, migration assays and invasion assays, respectively. The expressions of ZEB1, E-cadherin, and vimentin were observed by western blot. Results showed that DU145 cells exhibited a high expression of miR-200c compared with immortalized normal prostate epithelial cell RWPE-1. Du145 cells were then transfected with miR-200c mimics and displayed lower abilities of proliferation, migration, and invasion than those transfected with the negative control. The protein levels of ZEB1 and vimentin were expressed at a low extent in Du145 cells, which were transfected with miR-200c mimics; by contrast, E-cadherin was highly expressed. Hence, miR-200c could significantly inhibit the proliferation of the prostate cancer cell line Du145; likewise, miR-200c could inhibit migration and invasion by epithelial-mesenchymal transition.

  5. ATP inhibits Ins(1,4,5)P3-evoked Ca2+ release in smooth muscle via P2Y1 receptors.

    Science.gov (United States)

    MacMillan, D; Kennedy, C; McCarron, J G

    2012-11-01

    Adenosine 5'-triphosphate (ATP) mediates a variety of biological functions following nerve-evoked release, via activation of either G-protein-coupled P2Y- or ligand-gated P2X receptors. In smooth muscle, ATP, acting via P2Y receptors (P2YR), may act as an inhibitory neurotransmitter. The underlying mechanism(s) remain unclear, but have been proposed to involve the production of inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] by phospholipase C (PLC), to evoke Ca(2+) release from the internal store and stimulation of Ca(2+)-activated potassium (K(Ca)) channels to cause membrane hyperpolarization. This mechanism requires Ca(2+) release from the store. However, in the present study, ATP evoked transient Ca(2+) increases in only ∼10% of voltage-clamped single smooth muscle cells. These results do not support activation of K(Ca) as the major mechanism underlying inhibition of smooth muscle activity. Interestingly, ATP inhibited Ins(1,4,5)P(3)-evoked Ca(2+) release in cells that did not show a Ca(2+) rise in response to purinergic activation. The reduction in Ins(1,4,5)P(3)-evoked Ca(2+) release was not mimicked by adenosine and therefore, cannot be explained by hydrolysis of ATP to adenosine. The reduction in Ins(1,4,5)P(3)-evoked Ca(2+) release was, however, also observed with its primary metabolite, ADP, and blocked by the P2Y(1)R antagonist, MRS2179, and the G protein inhibitor, GDPβS, but not by PLC inhibition. The present study demonstrates a novel inhibitory effect of P2Y(1)R activation on Ins(1,4,5)P(3)-evoked Ca(2+) release, such that purinergic stimulation acts to prevent Ins(1,4,5)P(3)-mediated increases in excitability in smooth muscle and promote relaxation.

  6. Peroxisome proliferator-activated receptor-γ (PPAR-γ) agonist inhibits collagen synthesis in human hypertrophic scar fibroblasts by targeting Smad3 via miR-145

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Hua-Yu; Li, Chao; Zheng, Zhao; Zhou, Qin; Guan, Hao; Su, Lin-Lin; Han, Jun-Tao; Zhu, Xiong-Xiang; Wang, Shu-yue; Li, Jun, E-mail: lijunfmmu@163.com; Hu, Da-Hai, E-mail: hudahaifmmu@aliyun.com

    2015-03-27

    The transcription factor peroxisome proliferator-activated receptor-γ (PPAR-γ) functions to regulate cell differentiation and lipid metabolism. Recently, its agonist has been documented to regulate extracellular matrix production in human dermal fibroblasts. This study explored the underlying molecular mechanisms and gene interactions in hypertrophic scar fibroblasts (HSFBs) in vitro. HSFBs were cultured and treated with or without PPAR-γ agonist or antagonist for gene expression. Bioinformatical analysis predicted that miR-145 could target Smad3 expression. Luciferase assay was used to confirm such an interaction. The data showed that PPAR-γ agonist troglitazone suppressed expression of Smad3 and Col1 in HSFBs. PPAR-γ agonist induced miR-145 at the gene transcriptional level, which in turn inhibited Smad3 expression and Col1 level in HSFBs. Furthermore, ELISA data showed that Col1 level in HSFBs was controlled by a feedback regulation mechanism involved in PPAR-γ agonist and antagonist-regulated expression of miR-145 and Smad3 in HSFBs. These findings indicate that PPAR-γ-miR-145-Smad3 axis plays a role in regulation of collagen synthesis in HSFBs. - Highlights: • PPAR-γ agonist inhibits collagen synthesis in HSFBs. • Smad3 and type I collagen expression are decreased by PPAR-γ agonist. • miR-145 expression is increased by PPAR-γ agonist in HSFBs. • Increased miR-145 inhibits collagen synthesis by targeting Smad3. • miR-145 regulates collagen synthesis.

  7. Mapping the methylation status of the miR-145 promoter in saphenous vein smooth muscle cells from individuals with type 2 diabetes.

    Science.gov (United States)

    Riches, Kirsten; Huntriss, John; Keeble, Claire; Wood, Ian C; O'Regan, David J; Turner, Neil A; Porter, Karen E

    2017-03-01

    Type 2 diabetes mellitus prevalence is growing globally, and the leading cause of mortality in these patients is cardiovascular disease. Epigenetic mechanisms such as microRNAs (miRs) and DNA methylation may contribute to complications of type 2 diabetes mellitus. We discovered an aberrant type 2 diabetes mellitus-smooth muscle cell phenotype driven by persistent up-regulation of miR-145. This study aimed to determine whether elevated expression was due to changes in methylation at the miR-145 promoter. Smooth muscle cells were cultured from saphenous veins of 22 non-diabetic and 22 type 2 diabetes mellitus donors. DNA was extracted, bisulphite treated and pyrosequencing used to interrogate methylation at 11 CpG sites within the miR-145 promoter. Inter-patient variation was high irrespective of type 2 diabetes mellitus. Differential methylation trends were apparent between non-diabetic and type 2 diabetes mellitus-smooth muscle cells at most sites but were not statistically significant. Methylation at CpGs -112 and -106 was consistently lower than all other sites explored in non-diabetic and type 2 diabetes mellitus-smooth muscle cells. Finally, miR-145 expression per se was not correlated with methylation levels observed at any site. The persistent up-regulation of miR-145 observed in type 2 diabetes mellitus-smooth muscle cells is not related to methylation at the miR-145 promoter. Crucially, miR-145 methylation is highly variable between patients, serving as a cautionary note for future studies of this region in primary human cell types.

  8. Defining the minimal structural requirements for partial agonism at the type I myo-inositol 1,4,5-trisphosphate receptor.

    Science.gov (United States)

    Wilcox, R A; Fauq, A; Kozikowski, A P; Nahorski, S R

    1997-02-03

    The novel synthetic analogues D-3-fluoro-myo-inositol 1,5-bisphosphate-4-phosphorothioate, [3F-Ins(1,5)P2-4PS], D-3-fluoro-myo-inositol 1,4-bisphosphate-5-phosphorothioate [3F-Ins(1,4)P2-5PS], and D-3-fluoro-myo-inositol 1-phosphate-4,5-bisphosphorothioate [3F-Ins(1)P-(4,5)PS2] were utilised to define the structure-activity relationships which could produce partial agonism at the Ca2+ mobilising myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] receptor. Based on prior structure-activity data we hypothesised that the minimal structural requirements for lns(1,4,5)P3 receptor partial agonism, were phosphorothioate substitution of the crucial vicinal 4,5-bisphosphate pair accompanied by another structural perturbation, such fluorination of 3-position of the myo-inositol ring. All the analogues fully displaced [3H]Ins(1,4,5)P3 from a single Ins(1,4,5)P3 binding site in pig cerebellar membranes [3F-Ins(1,5)P2-4PS (1C50 = 26 nM), 3F-Ins(1,4)P2-5PS (IC50 = 80 nM) and 3F-Ins(1)P-(4,5)PS2 (IC50 = 109 nM) cf. Ins(1,4,5)P3 (IC50 = 11 nM)]. In contrast, 3F-Ins(1,5)P2-4PS (IC50 = 424 nM) and 3F-Ins(1,4)P2-5PS (IC50 = 3579 nM) were weak full agonists at the Ca2+ mobilising Ins(1,4,5)P3 receptor of permeabilised SH-SY5Y neuroblastoma cells, being respectively 4- and 36-fold less potent than Ins(1,4,5)P3 (EC50 = 99 nM). While 3F-Ins(1)P-(4,5)PS2 (EC50 = 11345 nM) was a partial agonist releasing only 64.3 +/- 1.9% of the Ins(1,4,5)P3-sensitive intracellular Ca2+ pools. 3F-Ins(1)P-(4,5)PS2 was unique among the Ins(1,4,5)P3 receptor partial agonists so far identified in having a relatively high affinity for the Ins(1,4,5)P3 binding site, accompanied by a significant loss of intrinsic activity for Ca2+ mobilisation. This improved affinity was probably due to the retention of the 1-position phosphate, which enhances interaction with the Ins-(1,4,5)P3 receptor. 3F-Ins(1)P-(4,5)PS2 may be an important lead compound for the development of efficient Ins(1,4,5)P3 receptor antagonists.

  9. Mutual induction of transcription factor PPARγ and microRNAs miR-145 and miR-329.

    Science.gov (United States)

    Dharap, Ashutosh; Pokrzywa, Courtney; Murali, Shruthi; Kaimal, Balarama; Vemuganti, Raghu

    2015-10-01

    MicroRNAs (miRNAs) are small non-coding RNAs that are known to control mRNA translation. Most miRNAs are transcribed from specific genes with well-defined promoters located throughout the genome. The mechanisms that control miRNA expression under normal and pathological conditions are not yet understood clearly. Peroxisome proliferator-activated receptor (PPAR) γ is a ligand-activated transcription factor that is extensively distributed in the CNS. PPARγ activation induces neuroprotection by modulating genes that contain peroxisome proliferator response elements (PPREs) in their promoters. We presently evaluated if PPARγ modulates miRNA expression. When adult rats were treated with PPARγ agonist rosiglitazone, expression of 28 miRNAs altered significantly (12 up- and 16 down-regulated; 3-119 fold) in the cerebral cortex compared to vehicle-treated controls. In silico analysis showed 1-5 PPREs in the putative promoter regions (within 1 Kb upstream of the transcription start site) of these miRNA genes. Cotransfection with a PPARγ constitutively expressing vector significantly induced the miR-145 and miR-329 promoter vectors (each have four PPREs), which was curtailed by point mutations of PPREs in their promoters. Interestingly, the PPARγ promoter has binding sites for both these miRNAs and transfection with miR-329 mimic and miR-145 mimic induced the PPARγ expression. Thus, these studies show a cyclical induction of miRNAs and PPARγ, indicating that the pleiotropic beneficial effects of PPARγ agonists might be modulated in part by miRNAs and their down-stream mRNAs. We proposed that promoters of many microRNAs contain the binding sites for the transcription factor PPARγ. Activation of PPARγ modulates the expression of these microRNAs. Two such PPARγ-responsive microRNAs (miR-145 and miR-329) bind to PPARγ promoter to induce its expression. This indicates the presence of a feedback loop by which transcription factors and microRNAs can modulate each other.

  10. Long non-coding RNA SNHG14 promotes microglia activation by regulating miR-145-5p/PLA2G4A in cerebral infarction.

    Science.gov (United States)

    Qi, Xu; Shao, Ming; Sun, Hongjing; Shen, Yue; Meng, Delong; Huo, Wei

    2017-04-21

    Activated microglia cells (MCs) are able to release a large amount of inflammatory cytokines after ischemic stroke, which exacerbates neuron damage. In this study, we explored the functional involvement of long non-coding RNA (lncRNA) SNHG14 and its potential regulatory mechanism in the activation of MCs. The mouse model of middle cerebral artery occlusion (MCAO) and microglia cell model of oxygen/glucose deprivation (OGD) were made. The expression of SNHG14, miR-145-5p and PLA2G4A protein expression was determined by quantitative real time PCR and western blot, respectively. Dual-luciferase assay was used to verify the direct binding of miR-145-5p and PLA2G4A. Flow cytometry was applied to measure neurons' apoptosis. SNHG14 highly expressed in ischemic cerebral tissues and BV-2 cells after OGD treatment. SNHG14 knockdown could remarkably inhibit BV-2 cells activation induced by OGD; while SNHG14 overexpression significantly promoted BV-2 cells activation, showing an increase of TNF-α and NO production and neurons' apoptosis rate. Additionally, SNHG14 knockdown promoted the expression of miR-145-5p and reduced PLA2G4A. Contrarily, SNHG14 overexpression inhibited miR-145-5p expression and increased PLA2G4A. Moreover, miR-145-5p overexpression also reversed the effect of OGD on BV-2 cells activation. Bioinformatics analysis and dual-luciferase assay supported that SNHG14 could bind directly to miR-145-5p and miR-145-5p-binding site was existed on 3'-UTR of PLA2G4A. MiR-145-5p mimic reversed the increase of PLA2G4A and reduced the high levels of TNF-α and NO in BV-2 cells induced by SNHG14 overexpression. SNHG14 increased the expression of PLA2G4A by inhibition of miR-145-5p, which resulted in the activation of MCs in cerebral infarction.

  11. Growth of Pollen Tubes of Papaver rhoeas Is Regulated by a Slow-Moving Calcium Wave Propagated by Inositol 1,4,5-Trisphosphate.

    Science.gov (United States)

    Franklin-Tong, V. E.; Drobak, B. K.; Allan, A. C.; Watkins, PAC.; Trewavas, A. J.

    1996-08-01

    A signaling role for cytosolic free Ca2+ ([Ca2+]i) in regulating Papaver rhoeas pollen tube growth during the self-incompatibility response has been demonstrated previously. In this article, we investigate the involvement of the phosphoinositide signal transduction pathway in Ca2+-mediated pollen tube inhibition. We demonstrate that P. rhoeas pollen tubes have a Ca2+-dependent polyphosphoinositide-specific phospholipase C activity that is inhibited by neomycin. [Ca2+]i imaging after photolysis of caged inositol (1,4,5)-trisphosphate (Ins[1,4,5]P3) in pollen tubes demonstrated that Ins(1,4,5)P3 could induce Ca2+ release, which was inhibited by heparin and neomycin. Mastoparan, which stimulated Ins(1,4,5)P3 production, also induced a rapid increase in Ca2+, which was inhibited by neomycin. These data provide direct evidence for the involvement of a functional phosphoinositide signal-transducing system in the regulation of pollen tube growth. We suggest that the observed Ca2+ increases are mediated, at least in part, by Ins(1,4,5)P3-induced Ca2+ release. Furthermore, we provide data suggesting that Ca2+ waves, which have not previously been reported in plant cells, can be induced in pollen tubes.

  12. Enantiopure 1,4,5-trisubstituted 1,2,3-triazoles from carbohydrates: applications of organoselenium chemistry.

    Science.gov (United States)

    Bhaumik, Atanu; Samanta, Supravat; Pathak, Tanmaya

    2014-08-01

    A wide range of stable vinyl selenone-modified furanosides has been synthesized for the first time. These 2π-partners undergo 1,3-dipolar cycloaddition reactions with a wide range of organic azides to afford enantiopure trisubstituted triazoles. Furanosyl rings opened up during triazole synthesis to generate polyfunctionalized molecules, ready to undergo further transformations. This strategy is one of the most convenient methods for the synthesis of enantiopure 1,4,5-trisubstituted 1,2,3-triazoles where the chiral components are attached to C-4 or C-5 position of triazole ring. These triazoles are formed in a regioselective manner, and several pairs of regioisomeric triazoles have also been synthesized. The approach affords densely functionalized triazoles, which are amenable to further modifications because of the presence of aldehyde and hydroxyl groups. This powerful and practical route adds to the arsenals of chemists and biologists interested in the synthesis and applications of triazoles.

  13. Caenorhabditis elegans Inositol 5-Phosphatase Homolog Negatively Regulates Inositol 1,4,5-Triphosphate Signaling in Ovulation V⃞

    Science.gov (United States)

    Bui, Yen Kim; Sternberg, Paul W.

    2002-01-01

    Ovulation in Caenorhabditis elegans requires inositol 1,4,5-triphosphate (IP3) signaling activated by the epidermal growth factor (EGF)-receptor homolog LET-23. We generated a deletion mutant of a type I 5-phosphatase, ipp-5, and found a novel ovulation phenotype whereby the spermatheca hyperextends to engulf two oocytes per ovulation cycle. The temporal and spatial expression of IPP-5 is consistent with its proposed inhibition of IP3 signaling in the adult spermatheca. ipp-5 acts downstream of let-23, and interacts with let-23–mediated IP3 signaling pathway genes. We infer that IPP-5 negatively regulates IP3 signaling to ensure proper spermathecal contraction. PMID:12006659

  14. Production of intense highly charged ion beams by IMP 14.5 GHz electron cyclotron resonance ion source

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A new 14.5 GHz Electron Cyclotron Resonance (ECR) ion source has been constructed over the last two years. The source was designed and tested by making use of the latest results from ECR ion source development, such as high mirror magnetic field, large plasma volume, and biased probe. 140μA of O7+, 185μA of Ar11+ and 50 μA of Xe26+ could be produced with a RF power of 800 W. The intense beams of highly charged metallic ions are produced by means of the method of a metal evaporation oven and volatile compound through axial access. The test results are 130μA of Ca11+, 70μA of Ca12+ and 65μA of Fe10+. The ion source has been put into operation for the cyclotron at the Institute of Modern Physics (IMP).

  15. Ultrastable fiber amplifier delivering 145-fs pulses with 6-μJ energy at 10-MHz repetition rate.

    Science.gov (United States)

    Wunram, Marcel; Storz, Patrick; Brida, Daniele; Leitenstorfer, Alfred

    2015-03-01

    A high-power femtosecond Yb:fiber amplifier operating with exceptional noise performance and long-term stability is demonstrated. It generates a 10-MHz train of 145-fs pulses at 1.03 μm with peak powers above 36 MW. The system features a relative amplitude noise of 1.5·10⁻⁶  Hz(-1/2) at 1 MHz and drifts of the 60-W average power below 0.3% over 72 hours of continuous operation. The passively phase-stable Er:fiber seed system provides ultrabroadband pulses that are synchronized at a repetition rate of 40 MHz. This combination aims at new schemes for sensitive experiments in ultrafast scientific applications.

  16. Pentamidine reduces expression of hypoxia-inducible factor-1α in DU145 and MDA-MB-231 cancer cells.

    Science.gov (United States)

    Jung, Hui-Jung; Suh, Seong-Il; Suh, Min-Ho; Baek, Won-Ki; Park, Jong-Wook

    2011-04-01

    Pentamidine is an aromatic diamine used for the treatment of human protozoa infections. Recently, pentamidine has been reported to exhibit anticancer properties. In this study, we report that pentamidine inhibits expression of hypoxia-inducible factor (HIF)-1α in cancer cells. Pentamidine decreased HIF-1α protein translation and enhanced its protein degradation in DU145 prostate cancer and MDA-MB-231 breast cancer cells. In parallel with reduction of de novo synthesis of HIF-1α, pentamidine was able to suppress global protein translation, an effect accompanied by the reduction of eIF4F complex formation and also the induction of eIF2α phosphorylation. These results show that pentamidine is a potential inhibitor of HIF-1α and its potential as a cancer therapeutic reagent warrants further study.

  17. Exposure-related effects of Pseudomonas fluorescens, strain CL145A, on coldwater, coolwater, and warmwater fish

    Science.gov (United States)

    Luoma, James A.; Weber, Kerry L.; Denise A. Mayer,

    2015-01-01

    The exposure-related effects of a commercially prepared spray-dried powder (SDP) formulation of Pseudomonas fluorescens, strain CL145A, were evaluated on coldwater, coolwater, and warmwater fish endemic to the Great Lakes and Upper Mississippi River Basins. Nine species of young-of-the-year fish were exposed to SDP for 24 hours by using continuous-flow, serial-dilution exposure systems at temperatures of 12 degrees Celsius (°C; 2 species; Oncorhynchus mykiss [rainbow trout] and Salvelinus fontinalis [brook trout]), 17 °C (3 species; Perca flavescens [yellow perch], Sander vitreus [walleye], and Acipenser fulvescens [lake sturgeon]), or 22 °C (4 species; Micropterus salmoides [largemouth bass], Micropterus dolomieu [smallmouth bass], Lepomis macrochirus [bluegill sunfish], and Ictalurus punctatus [channel catfish]).

  18. Exposure-related effects of formulated Pseudomonas fluorescens strain CL145A to glochidia from seven unionid mussel species

    Science.gov (United States)

    Luoma, James A.; Weber, Kerry L.; Severson, Todd J.; Schreier, Theresa M.; Mayer, Denise A.; Aloisi, Douglas B.; Eckert, Nathan L.

    2015-01-01

    The study was completed to evaluate the exposure-related effects of a biopesticide for dreissenid mussel (Dreissena polymorpha, zebra mussel and Dreissena rostriformis bugensis, quagga mussel) control on glochidia from unionid mussels endemic to the Great Lakes and Upper Mississippi River Basins. The commercially prepared biopesticide was either a spray-dried powder (SDP) or freeze-dried powder (FDP) formulation of Pseudomonas fluorescens, strain CL145A. Glochidia of the unionid mussel species Lampsilis cardium, Lampsilis siliquoidea,Lampsilis higginsii, Ligumia recta, Obovaria olivaria, and Actinonaias ligamentina were exposed to SDP-formulated P. fluorescens andLampsilis cardium and Megalonaias nervosa were exposed to FDP-formulated P. fluorescens.

  19. Identification, purification, characterization and regulation of the rabbit peritoneal neutrophil cytosolic inositol 1,4,5-trisphosphate 5-phosphomonoesterase

    Energy Technology Data Exchange (ETDEWEB)

    Kennedy, S.P.

    1988-01-01

    Inositol 1,4,5-trisphosphate (IP{sub 3}) is a second messenger involved in intracellular Ca{sup 2+} mobilization. Its enzymatic breakdown by inositol 1,4,5-trisphosphate 5-phosphomonoesterase (IP{sub 3} phosphatase) yields inositol 1,4-bisphosphate (IP{sub 2}) which does not mobilize Ca{sup 2+}. Thus, the IP{sub 3} phosphatase can serve to regulate internal free Ca{sup 2+} levels. In Triton X-100 permeabilized rabbit peritoneal neutrophils and neutrophil cytosol, exogenously added ({sup 3}H)IP{sub 3} is rapidly hydrolyzed to IP{sub 2}, inositol monophosphate (IP) and free inositol. The rate of IP{sub 3} hydrolysis was greater than that of IP{sub 2} in permeabilized neutrophils, while the converse was observed in cytosol. DE-52 chromatography of cytosol separates the specific from nonspecific IP{sub 3} phosphatase activity. Further purification of the specific enzyme resulted in a 790-fold purification over cytosol activity, however, the IP{sub 3} phosphatase could not be identified with any protein in this preparation. The neutrophil IP{sub 3} phosphatase has a molecular weight of 43-47 kDa, and an isoelectric point of 5.6, as determined by size exclusion chromatography and Chromatofocusing, respectively. Physiological concentrations of Ca{sup 2+} and calmodulin have no effect on IP{sub 3} phosphatase activity. Activation of endogenous protein kinase C in permeabilized cells and cytosol also has no effect on the activity. Characteristics of the neutrophil IP{sub 3} phosphatase are discussed in relation to IP{sub 3} phosphatases in other cells and tissues.

  20. Dynamic expression of MEIS1 homeoprotein in E14.5 forebrain and differentiated forebrain-derived neural stem cells.

    Science.gov (United States)

    Barber, Benjamin A; Liyanage, Vichithra R B; Zachariah, Robby M; Olson, Carl O; Bailey, Melissa A G; Rastegar, Mojgan

    2013-10-01

    Central nervous system development is controlled by highly conserved homeoprotein transcription factors including HOX and TALE (Three Amino acid Loop Extension). TALE proteins are primarily known as HOX-cofactors and play key roles in cell proliferation, differentiation and organogenesis. MEIS1 is a TALE member with established expression in the developing central nervous system. MEIS1 is essential for embryonic development and Meis1 knockout mice dies at embryonic day (E) 14.5. However, Meis1/MEIS1 expression in the devolving forebrain, at this critical time-point has not been studied. Here, for the first time we characterize the region-specific expression of MEIS1 in E14.5 mouse forebrain, filling the gap of MEIS1 expression profile between E12.5 and E16.5. Previously, we reported MEIS1 transcriptional regulatory role in neuronal differentiation and established forebrain-derived neural stem cells (NSC) for gene therapy application of neuronal genes. Here, we show the dynamic expression of Meis1/MEIS1 during the differentiation of forebrain-derived NSC toward a glial lineage. Our results show that Meis1/MEIS1 expression is induced during NSC differentiation and is expressed in both differentiated neurons and astrocytes. Confirming these results, we detected MEIS1 expression in primary cultures of in vivo differentiated cortical neurons and astrocytes. We further demonstrate Meis1/MEIS1 expression relative to other TALE family members in the forebrain-derived NSC in the absence of Hox genes. Our data provide evidence that forebrain-derived NSC can be used as an accessible in vitro model to study the expression and function of TALE proteins, supporting their potential role in modulating NSC self-renewal and differentiation.

  1. Role of inositol 1,4,5-trisphosphate receptors in α1-adrenergic receptor-induced cardiomyocyte hypertrophy

    Institute of Scientific and Technical Information of China (English)

    Da-li LUO; Jian GAO; Xiao-mei LAN; Gang WANG; Sheng WEI; Rui-ping XIAO; Qi-de HAN

    2006-01-01

    Aim: Intracellular Ca2+ plays pivotal roles in diverse cellular functions, including gene transcription that underlies cardiac remodeling during stress responses. However, the role of inositol 1,4,5-trisphosphate receptors (IP3Rs) in the mediation of cardiac intracellular Ca2+ and hypertrophic growth remains elusive. Prior work with neonatal rat ventricular myocytes suggests that activation of IP3Rs may be linked to α1 adrenergic receptor (α1AR) increased stereotyped Ca2+ spark occurrence and global Ca2+ oscillations. Thus, we hypothesized that Ca2+ release through IP3Rs was necessary for α1AR-stimulated cardiac hypertrophy. Methods: We used myoinositol 1,4,5-trisphosphate hexakis (butyryloxymethyl) ester (IP3BM), a membrane-permeant ester of IP3, to activate IP3Rs directly, and Fluo 4/AM to measure intracellular Ca2+ signaling. Results: IP3BM (10μmol·L-1) mimicked the effects of phenylephrine, a selective agonist of α1AR, in increments in local Ca2+ spark release (especially in the perinuclear area) and global Ca2+ transient frequencies. More importantly, IP3R inhibitors, 2-aminoethoxydiphenyl borate and Xestospongin C, abolished the IP3BM-induced Ca2+ responses, and significantly suppressed α1AR-induced cardiomyocyte hypertrophy assayed by cell size, [3H] leucine incorporation and atrial natriuretic factor gene expression, during sustained (48 h) phenylephrine stimulation. Conclusion: These results, therefore, provide cellular mechanisms that link IP3R signaling to α1AR-stimulated gene expression and cardiomyocyte hypertrophy.

  2. Corrective Action Decision Document for Corrective Action Unit 145: Wells and Storage Holes, Nevada Test Site, Nevada, Rev. No.: 0, with ROTC No. 1 and Addendum

    Energy Technology Data Exchange (ETDEWEB)

    David Strand

    2006-04-01

    This Corrective Action Decision Document has been prepared for Corrective Action Unit (CAU) 145, Wells and Storage Holes in Area 3 of the Nevada Test Site, Nevada, in accordance with the ''Federal Facility Agreement and Consent Order'' (1996). Corrective Action Unit 145 is comprised of the following corrective action sites (CASs): (1) 03-20-01, Core Storage Holes; (2) 03-20-02, Decon Pad and Sump; (3) 03-20-04, Injection Wells; (4) 03-20-08, Injection Well; (5) 03-25-01, Oil Spills; and (6) 03-99-13, Drain and Injection Well. The purpose of this Corrective Action Decision Document is to identify and provide the rationale for the recommendation of a corrective action alternative for the six CASs within CAU 145. Corrective action investigation activities were performed from August 1, 2005, through November 8, 2005, as set forth in the CAU 145 Corrective Action Investigation Plan and Record of Technical Change No. 1. Analytes detected during the Corrective Action Investigation (CAI) were evaluated against appropriate final action levels to identify the contaminants of concern for each CAS. The results of the CAI identified contaminants of concern at one of the six CASs in CAU 145 and required the evaluation of corrective action alternatives. Assessment of the data generated from investigation activities conducted at CAU 145 revealed the following: CASs 03-20-01, 03-20-02, 03-20-04, 03-20-08, and 03-99-13 do not contain contamination; and CAS 03-25-01 has pentachlorophenol and arsenic contamination in the subsurface soils. Based on the evaluation of analytical data from the CAI, review of future and current operations at the six CASs, and the detailed and comparative analysis of the potential corrective action alternatives, the following corrective actions are recommended for CAU 145. No further action is the preferred corrective action for CASs 03-20-01, 03-20-02, 03-20-04, 03-20-08, and 03-99-13. Close in place is the preferred corrective action

  3. Search for a standard-model-like Higgs boson with a mass in the range 145 to 1000 GeV at the LHC.

    Science.gov (United States)

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Ross, I; Savin, A; Smith, W H; Swanson, J

    A search for a standard-model-like Higgs boson in the H→WW and H→ZZ decay channels is reported, for Higgs boson masses in the range 145LHC. The combined upper limits at 95 % confidence level on products of the cross section and branching fractions exclude a standard-model-like Higgs boson in the range 145

  4. Human amniotic epithelial cell feeder layers maintain human iPS cell pluripotency via inhibited endogenous microRNA-145 and increased Sox2 expression

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Te, E-mail: liute79@yahoo.com [School of Environmental Science and Engineering, Donghua University, Shanghai 201620 (China); Shanghai Geriatric Institute of Chinese Medicine, Shanghai 200031 (China); Cheng, Weiwei [International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University, Shanghai 200030 (China); Huang, Yongyi [Laboratoire PROTEE, Batiment R, Universite du Sud Toulon-Var, 83957 LA GARDE Cedex (France); Huang, Qin; Jiang, Lizhen [Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China); Guo, Lihe, E-mail: liute79@yahoo.com [Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China)

    2012-02-15

    Currently, human induced pluripotent stem (iPS) cells were generated from patient or disease-specific sources and share the same key properties as embryonic stem cells. This makes them attractive for personalized medicine, drug screens or cellular therapy. Long-term cultivation and maintenance of normal iPS cells in an undifferentiated self-renewing state are a major challenge. Our previous studies have shown that human amniotic epithelial cells (HuAECs) could provide a good source of feeder cells for mouse and human embryonic stem cells, or spermatogonial stem cells, but the mechanism for this is unknown. Here, we examined the effect of endogenous microRNA-145 regulation on Sox2 expression in human iPS cells by HuAECs feeder cells regulation, and in turn on human iPS cells pluripotency. We found that human IPS cells transfected with a microRNA-145 mutant expressed Sox2 at high levels, allowing iPS to maintain a high level of AP activity in long-term culture and form teratomas in SCID mice. Expression of stem cell markers was increased in iPS transfected with the microRNA-145 mutant, compared with iPS was transfected with microRNA-145. Besides, the expression of Drosha proteins of the microRNA-processor complex, required for the generation of precursor pre-miRNA, was significantly increased in human iPS cells cultured on MEF but not on HuAECs. Taken together, these results suggest that endogenous Sox2 expression may be regulated by microRNA-145 in human iPS cells with HuAECs feeder cells, and Sox2 is a crucial component required for maintenance of them in an undifferentiated, proliferative state capable of self-renewal. Highlights: Black-Right-Pointing-Pointer microRNA-145 inhibits Sox2 expression in human iPS cells. Black-Right-Pointing-Pointer microRNA-145 suppresses the self-renewal and pluripotency of human iPS cells. Black-Right-Pointing-Pointer HuAECs regulate expression of microRNA-145 and Sox2 in human iPS cells. Black-Right-Pointing-Pointer HuAECs feeder

  5. The Role of VP1 Amino Acid Residue 145 of Enterovirus 71 in Viral Fitness and Pathogenesis in a Cynomolgus Monkey Model.

    Directory of Open Access Journals (Sweden)

    Chikako Kataoka

    2015-07-01

    Full Text Available Enterovirus 71 (EV71, a major causative agent of hand, foot, and mouth disease, occasionally causes severe neurological symptoms. We identified P-selectin glycoprotein ligand-1 (PSGL-1 as an EV71 receptor and found that an amino acid residue 145 in the capsid protein VP1 (VP1-145 defined PSGL-1-binding (PB and PSGL-1-nonbinding (non-PB phenotypes of EV71. However, the role of PSGL-1-dependent EV71 replication in neuropathogenesis remains poorly understood. In this study, we investigated viral replication, genetic stability, and the pathogenicity of PB and non-PB strains of EV71 in a cynomolgus monkey model. Monkeys were intravenously inoculated with cDNA-derived PB and non-PB strains of EV71, EV71-02363-EG and EV71-02363-KE strains, respectively, with two amino acid differences at VP1-98 and VP1-145. Mild neurological symptoms, transient lymphocytopenia, and inflammatory cytokine responses, were found predominantly in the 02363-KE-inoculated monkeys. During the early stage of infection, viruses were frequently detected in clinical samples from 02363-KE-inoculated monkeys but rarely in samples from 02363-EG-inoculated monkeys. Histopathological analysis of central nervous system (CNS tissues at 10 days postinfection revealed that 02363-KE induced neuropathogenesis more efficiently than that induced by 02363-EG. After inoculation with 02363-EG, almost all EV71 variants detected in clinical samples, CNS, and non-CNS tissues, possessed a G to E amino acid substitution at VP1-145, suggesting a strong in vivo selection of VP1-145E variants and CNS spread presumably in a PSGL-1-independent manner. EV71 variants with VP1-145G were identified only in peripheral blood mononuclear cells in two out of four 02363-EG-inoculated monkeys. Thus, VP1-145E variants are mainly responsible for the development of viremia and neuropathogenesis in a non-human primate model, further suggesting the in vivo involvement of amino acid polymorphism at VP1-145 in cell

  6. MicroRNA-145在乳腺癌中的表达及其对乳腺癌侵袭转移的影响%Expression of miR-145 in breast cancer and its role in invasion and migration of breast cancer cells

    Institute of Scientific and Technical Information of China (English)

    刘水逸; 吴唐维; 李晓怡; 陈卫群; 孔庆志; 卢忠心

    2015-01-01

    目的:探讨微小RNA-145( microRNA-145,miR-145)在乳腺癌组织和细胞系中的表达及其与乳腺癌侵袭转移的关系。方法回顾性研究。用实时荧光定量PCR( FQ-PCR)检测39例乳腺癌组织及其对应癌旁组织以及1株乳腺上皮细胞系和4株乳腺癌细胞系的miR-145的表达;应用脂质体将miR-145表达质粒( pSIF-miR-145)及阴性对照( pSIF空白载体)转染人乳腺癌细胞系SK-BR-3,应用划痕和Transwell实验检测miR-145对细胞迁移及侵袭能力的影响;应用生物信息学分析预测miR-145的靶基因,用报告基因分析验证促血管生成素2( ANGPT2)为其靶基因,并用免疫印迹Western blot检测miR-145对ANGPT2蛋白表达的影响。结果乳腺癌组织中miR-145的相对表达量为45.93±22.02,癌旁组织的miR-145相对表达量为182.04±56.92,乳腺癌组织中miR-145的表达显著低于癌旁组织(U值为7, P<0.01)。乳腺癌细胞系(MCF-7、MDA-MB-231、MDA-MB-468和SK-B-3)中miR-145相对表达依次为0.51±0.05、0.07±0.01、0.36±0.04、0.04±0.01,与正常对照相比,所有乳腺癌细胞系中 miR-145表达均降低( t 值分别为15.93、308.17、25.02、201.30, P 均<0.05)。同时高侵袭转移乳腺癌细胞系( MDA-MB-231、MDA-MB-468和SK-B-3)中miR-145的相对表达量显著低于低侵袭转移乳腺癌细胞系(MCF-7)(t值分别为14.18、3.78、15.20, P均<0.05)。在SK-BR-3细胞中过表达miR-145后,划痕实验显示划痕愈合速度明显慢于阴性对照组。侵袭实验显示上调miR-145后穿过基质胶的平均细胞数为(137±37)个,对照组为(617±80)个,侵袭能力明显减弱( P<0.01)。高表达miR-145能够抑制ANGPT2蛋白的表达;miR-145能够抑制ANGPT23′UTR的荧光素酶活性,突变miR-145

  7. Antibody to a 145-kilodalton outer membrane protein has bactericidal activity and protective activity against experimental bacteremia caused by a Brazilian purpuric fever isolate of Haemophilus influenzae biogroup aegyptius. The Brazilian Purpuric Fever Study Group.

    Science.gov (United States)

    Rubin, L G; Rizvi, A

    1991-12-01

    The immunologic basis for protection against Brazilian purpuric fever, a septicemic infection associated with Haemophilus influenzae biogroup aegyptius bacteremia, is unknown. Passive immunization of infant rats with antiserum to whole bacterial cells of the homologous strain protects them from experimental bacteremia following bacterial challenge. In immunoblotting, antibody to a 145-kDa protein (P145) was present in protective antisera but not in nonprotective antisera. As judged by analysis of the antibodies eluted from whole bacterial cells and the agglutination of bacteria by antisera to P145, this protein is surface exposed. We prepared monospecific rat antisera to this protein by three methods: (i) immunization with whole bacterial cells and absorption with a Brazilian purpuric fever strain not expressing P145, (ii) immunization with gel-purified P145, and (iii) immunization with a P145-expressing transformant of a laboratory H. influenzae strain expressing this protein and absorption of the antiserum with the laboratory H. influenzae strain. These antisera had low antilipooligosaccharide antibody titers, were reactive only with P145, and had bactericidal activity in vitro. Following passive immunization, these antisera partially protected infant rats from bacteremia resulting from intraperitoneal challenge with bacteria. As assessed by immunoblotting, pooled adult human sera contained antibodies reactive with P145. Antibody to P145 may contribute to protection against Brazilian purpuric fever.

  8. 手机辐射对Mark145细胞生长的影响%The Influence of the Cellular Phone Radiation on the Growth of Mark145 Cell

    Institute of Scientific and Technical Information of China (English)

    李瑞芳; 孙婕; 杨丽丽

    2007-01-01

    目的 探讨手机辐射对生物细胞生长的影响.方法 以手机工作25min后休息5min作为一个循环.将Mark145单体细胞分三组,辐射时间分为:二个循环、四个循环、五个循环三种,分别将手机贴近细胞瓶和放在距离细胞瓶10cm处进行辐射处理.结果 经过辐射后培养3d,细胞明显死亡..继续培养3d后,细胞几乎没有存活.结论 手机的辐射作用对单层细胞具有致死作用,并且辐射时间越长,距离越近,细胞死亡的数量也越多.

  9. The expression pattern of miR-142-3p and miR-145-3p in rat tissues%大鼠miR-142-3p、miR-145-3p表达模式分析

    Institute of Scientific and Technical Information of China (English)

    吴慧; 赵宇龙; 王艳红; 房兴堂; 陈宏; 张春雷

    2016-01-01

    Objective To screen and identify the key miRNAs during mammary gland development and milk secretion of rats. Methods Gene U6 was taken as interior label gene by real time-PCR to compare the differences of expression levels of miR-142-3p and miR-145-3p in the mammary gland, liver, heart, spleen, lung, kidney, ovary and uterus after 21 postpartum. Moreover, the expressions of miR-142-3p and miR-145-3p in different stages (1, 7 and 21 d) of lactation were summarized. Results There was significant difference in miR-142-3p in lactation 21 d between different tissues. The expression of miR-142-3p was significantly higher in mammary gland than that in heart, spleen, lung, kidney, ovary and uterus tissues, which was second only to the expression in liver (P0.05). Furthermore, the relative expression level of miR-142-3p was continuing downward continued to decline in breast at different stages of lactation, while the relative expression level of miR-145-3p was up-regulated after down-regulating. Conclusion miR-142-3p and miR-145-3p are differentially expressed in different tissues and physiological periods in rats. In addition, miR-142-3p can regulate the growth of mammary gland and the formation of lactation by targeting prolactin receptor (Prlr), miR-145-3p may have the same function with miR-145 and miR-145-5p.%目的:筛选并鉴定对大鼠乳腺发育和泌乳调控起关键作用的微RNAs(miRNAs)。方法以U6为内参基因,运用实时荧光定量PCR,比较miR-142-3p、miR-145-3p在泌乳21 d大鼠乳腺、肝、心、脾、肺、肾、卵巢、子宫各器官的组织样品表达量的差异及不同泌乳阶段(1、7、21 d)乳腺组织miR-142-3p、miR-145-3p的表达规律。结果miR-142-3p在泌乳21 d各组织的表达存在差异,乳腺中的表达量显著高于心、脾、肺、肾、卵巢和子宫,仅次于肝中的表达量(P0.05)。乳腺组织在泌乳1、7、21 d比较,miR-142-3p的相对表达量持续下调,而miR-145-3p

  10. Functional Role of Intracellular Calcium Receptor Inositol 1,4,5-Trisphosphate Type 1 in Rat Hippocampus after Neonatal Anoxia

    Science.gov (United States)

    Ikebara, Juliane Midori; Takada, Silvia Honda; Cardoso, Débora Sterzeck; Dias, Natália Myuki Moralles; de Campos, Beatriz Crossiol Vicente; Bretherick, Talitha Amanda Sanches; Higa, Guilherme Shigueto Vilar; Ferraz, Mariana Sacrini Ayres

    2017-01-01

    Anoxia is one of the most prevalent causes of neonatal morbidity and mortality, especially in preterm neonates, constituting an important public health problem due to permanent neurological sequelae observed in patients. Oxygen deprivation triggers a series of simultaneous cascades, culminating in cell death mainly located in more vulnerable metabolic brain regions, such as the hippocampus. In the process of cell death by oxygen deprivation, cytosolic calcium plays crucial roles. Intracellular inositol 1,4,5-trisphosphate receptors (IP3Rs) are important regulators of cytosolic calcium levels, although the role of these receptors in neonatal anoxia is completely unknown. This study focused on the functional role of inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) in rat hippocampus after neonatal anoxia. Quantitative real-time PCR revealed a decrease of IP3R1 gene expression 24 hours after neonatal anoxia. We detected that IP3R1 accumulates specially in CA1, and this spatial pattern did not change after neonatal anoxia. Interestingly, we observed that anoxia triggers translocation of IP3R1 to nucleus in hippocampal cells. We were able to observe that anoxia changes distribution of IP3R1 immunofluorescence signals, as revealed by cluster size analysis. We next examined the role of IP3R1 in the neuronal cell loss triggered by neonatal anoxia. Intrahippocampal injection of non-specific IP3R1 blocker 2-APB clearly reduced the number of Fluoro-Jade C and Tunel positive cells, revealing that activation of IP3R1 increases cell death after neonatal anoxia. Finally, we aimed to disclose mechanistics of IP3R1 in cell death. We were able to determine that blockade of IP3R1 did not reduced the distribution and pixel density of activated caspase 3-positive cells, indicating that the participation of IP3R1 in neuronal cell loss is not related to classical caspase-mediated apoptosis. In summary, this study may contribute to new perspectives in the investigation of

  11. Magnetic-Field-Induced Soft-Mode Quantum Phase Transition in the High-Temperature Superconductor La1.855Sr0.145CuO4

    DEFF Research Database (Denmark)

    Chang, J.; Christensen, Niels Bech; Niedermayer, C.

    2009-01-01

    Inelastic neutron-scattering experiments on the high-temperature superconductor La1.855Sr0.145CuO4 reveal a magnetic excitation gap Delta that decreases continuously upon application of a magnetic field perpendicular to the CuO2 planes. The gap vanishes at the critical field required to induce long...

  12. Downregulation of anti-oncomirs miR-143/145 cluster occurs before APC gene aberration in the development of colorectal tumors.

    Science.gov (United States)

    Kamatani, Akemi; Nakagawa, Yoshihito; Akao, Yukihiro; Maruyama, Naoko; Nagasaka, Mitsuo; Shibata, Tomoyuki; Tahara, Tomomitsu; Hirata, Ichiro

    2013-09-01

    Accumulating data indicate that some microRNAs (miRNAs or miRs) can function as tumor suppressors or oncogenes and as such are important in cancer development. We previously reported that miR-143 and -145 are frequently downregulated in colon adenomas and cancers, acting as tumor suppressors. In this present study, we investigated the relationship between the downregulation of the miR-143/145 cluster and genetic aberrations of adenomatous polyposis coli (APC), which are early genetic events in the development of colorectal tumors. The expression levels of both miRs were determined by performing real-time PCR on tissue samples of familial adenomatous polyposis (FAP), colorectal adenoma, colorectal cancer, and paired non-tumorous tissues. Also, the expression of C- or N-terminus of the APC protein and that of the p53 protein in these tissues were examined immunohistochemically. Our data clearly indicated that the decreased expression of miR-143 and -145 frequently occurred before APC gene aberrations. The downregulation of miR-143 and -145 is thus an important genetic event for the initiation step in colorectal tumor development.

  13. Oral vitamins C and E as additional treatment in patients with acute anterior uveitis: a randomised double masked study in 145 patients

    NARCIS (Netherlands)

    J. van Rooij (Jeroen); S.G. Schwartzenberg; G.S. Baarsma (Seerp); P.G.H. Mulder (Paul)

    1999-01-01

    textabstractAIM: To investigate the effect of additional oral vitamins C and E on acute anterior uveitis. METHODS: A placebo controlled double masked study on the effect of vitamin C 500 mg in combination with vitamin E 100 mg twice daily in 145 patients with acute ante

  14. MicroRNAs miR-143 and miR-145 modulate cytoskeletal dynamics and responsiveness of smooth muscle cells to injury

    Science.gov (United States)

    Xin, Mei; Small, Eric M.; Sutherland, Lillian B.; Qi, Xiaoxia; McAnally, John; Plato, Craig F.; Richardson, James A.; Bassel-Duby, Rhonda; Olson, Eric N.

    2009-01-01

    Vascular injury triggers dedifferentiation and cytoskeletal remodeling of smooth muscle cells (SMCs), culminating in vessel occlusion. Serum response factor (SRF) and its coactivator, myocardin, play a central role in the control of smooth muscle phenotypes by regulating the expression of cytoskeletal genes. We show that SRF and myocardin regulate a cardiovascular-specific microRNA (miRNA) cluster encoding miR-143 and miR-145. To assess the functions of these miRNAs in vivo, we systematically deleted them singly and in combination in mice. Mice lacking both miR-143 and miR-145 are viable and do not display overt abnormalities in smooth muscle differentiation, although they show a significant reduction in blood pressure due to reduced vascular tone. Remarkably, however, neointima formation in response to vascular injury is profoundly impeded in mice lacking these miRNAs, due to disarray of actin stress fibers and diminished migratory activity of SMCs. These abnormalities reflect the regulation of a cadre of modulators of SRF activity and actin dynamics by miR-143 and miR-145. Thus, miR-143 and miR-145 act as integral components of the regulatory network whereby SRF controls cytoskeletal remodeling and phenotypic switching of SMCs during vascular disease. PMID:19720868

  15. Reconstitution of the type-1 active site of the H145G/A variants of nitrite reductase by ligand insertion

    NARCIS (Netherlands)

    Wijma, HJ; Boulanger, MJ; Molon, A; Fittipaldi, M; Huber, M; Murphy, MEP; Verbeet, MP; Canters, GW

    2003-01-01

    Variants of the copper-containing nitrite reductase (NiR) of Alcaligenes faecalis S6 were constructed by site-directed mutagenesis, by which the C-terminal histidine ligand (His145) of the Cu in the type-1 site was replaced by an alanine or a glycine. The type-1 sites in the NiR variants as isolated

  16. Prior Activation of Inositol 1,4,5-Trisphosphate Receptors Suppresses the Subsequent Induction of Long-Term Potentiation in Hippocampal CA1 Neurons

    Science.gov (United States)

    Fujii, Satoshi; Yamazaki, Yoshihiko; Goto, Jun-Ichi; Fujiwara, Hiroki; Mikoshiba, Katsuhiko

    2016-01-01

    We investigated the role of inositol 1,4,5-trisphosphate receptors (IP3Rs) activated by preconditioning low-frequency afferent stimulation (LFS) in the subsequent induction of long-term potentiation (LTP) in CA1 neurons in hippocampal slices from mature guinea pigs. Induction of LTP in the field excitatory postsynaptic potential or the population…

  17. Role of inositol (1,4,5)trisphosphate in epidermal growth factor-induced Ca2+ signaling in A431 cells

    DEFF Research Database (Denmark)

    Hughes, A R; Bird, G S; Obie, J F;

    1991-01-01

    but significant Ca2+ signal after the addition of bradykinin. Experiments were designed to determine whether the Ca2+ response to epidermal growth factor after bradykinin results from mobilization of Ca2+ by an inositol 1,4,5-trisphosphate-independent mechanism. Epidermal growth factor stimulated additional...

  18. Amygdalin induces apoptosis through regulation of Bax and Bcl-2 expressions in human DU145 and LNCaP prostate cancer cells.

    Science.gov (United States)

    Chang, Hyun-Kyung; Shin, Mal-Soon; Yang, Hye-Young; Lee, Jin-Woo; Kim, Young-Sick; Lee, Myoung-Hwa; Kim, Jullia; Kim, Khae-Hawn; Kim, Chang-Ju

    2006-08-01

    Prostate cancer is one of the most common non-skin cancers in men. Amygdalin is one of the nitrilosides, natural cyanide-containing substances abundant in the seeds of plants of the prunasin family that have been used to treat cancers and relieve pain. In particular, D-amygdalin (D-mandelonitrile-beta-D-gentiobioside) is known to exhibit selective killing effect on cancer cells. Apoptosis, programmed cell death, is an important mechanism in cancer treatment. In the present study, we prepared the aqueous extract of the amygdalin from Armeniacae semen and investigated whether this extract induces apoptotic cell death in human DU145 and LNCaP prostate cancer cells. In the present results, DU145 and LNCaP cells treated with amygdalin exhibited several morphological characteristics of apoptosis. Treatment with amygdalin increased expression of Bax, a pro-apoptotic protein, decreased expression of Bcl-2, an anti-apoptotic protein, and increased caspase-3 enzyme activity in DU145 and LNCaP prostate cancer cells. Here, we have shown that amygdalin induces apoptotic cell death in human DU145 and LNCaP prostate cancer cells by caspase-3 activation through down-regulation of Bcl-2 and up-regulation of Bax. The present study reveals that amygdalin may offer a valuable option for the treatment of prostate cancers.

  19. 子宫内膜异位症中miR-143、miR-145和miR-126的差异表达%Differential expression of miR-143,miR-145 and miR-126 in endometriosis

    Institute of Scientific and Technical Information of China (English)

    郑冰冰; 徐超逸; 赵益萍; 韩灵芝; 虞楼超; 段萍

    2016-01-01

    目的 探索子宫内膜异位症(EMs)中miR-143、miR-145和miR-126的差异表达,以及不同月经周期的影响.方法 利用real time RT-PCR技术,比较miR-143、miR-145和miR-126在EMs患者在位内膜(EU,2例)、异位内膜(EC,14例)、配对EU+EC(14例),与非EMs患者内膜(EN,28例)中的表达差异;并分析EN和EC组不同月经周期对miR-143、miR-145和miR-126表达的影响.结果 不同月经周期的影响分析,发现仅EN标本中miR-143增生期表达量高于分泌期,差异有统计学意义(P<0.05).与EN相比,miR-143和miR-145在EU中均表达上调(P<0.05),miR-143、miR-145和miR-126在EC中均表达上调(P<0.01).配对EU-EC中,miR-143、miR-145和miR-126在EC中均表达上调(P<0.01).结论 miR-143、miR-145和miR-126在EMs中表达高于非EMs,在EC中表达高于EU.

  20. Circulating miR-145 is associated with plasma high-sensitivity C-reactive protein in acute ischemic stroke patients.

    Science.gov (United States)

    Jia, Lihua; Hao, Fang; Wang, Weihua; Qu, Yang

    2015-07-01

    Stroke is a major cerebrovascular disease threatening human health and life with high morbidity, disability and mortality. We aimed to find effective biomarkers for the early diagnosis on stroke. Nine previously reported stroke-associated miRNAs (miR-21, miR-23a, miR-29b, miR-124, miR-145, miR-210, miR-221, miR-223 and miR-483-5p) were measured by quantitative real time-PCR, and plasma high-sensitivity C-reactive protein (hs-CRP) and serum interleukin 6 (IL-6), the pro-inflammation markers in brain injury, were examined by enzyme-linked immunosorbent assay in 146 acute ischemic stroke patients and 96 healthy blood donors. We found that serum miR-145 was significantly increased within 24 h after stroke onset and serum miR-23a and miR-221 were decreased in patients. Moreover, serum miR-145 was strong positively correlated with plasma hs-CRP and moderate positively correlated with serum IL-6. Meanwhile, serum miR-23a and miR-221 were moderate negatively correlated with plasma hs-CRP but not serum IL-6. Importantly, the combination of hs-CRP and serum miR-145 gained a better sensitivity/spectivity for prediction of acute ischemia stroke (area under receiver operating characteristic curve from 0.794 to 0.896). Conclusively, our preliminary findings indicate that serum miR-145 upregulated in acute ischemic stroke might be a new biomarker for acute ischemia stroke evaluation. Copyright © 2015 John Wiley & Sons, Ltd.

  1. An rs4705342 T>C polymorphism in the promoter of miR-143/145 is associated with a decreased risk of ischemic stroke

    Science.gov (United States)

    Wei, Ye-Sheng; Xiang, Yang; Liao, Pin-Hu; Wang, Jun-Li; Peng, You-Fan

    2016-01-01

    The expression of miR-143/miR-145 was up-regulated in ischemic stroke (IS), which may be used as biomarkers and/or therapeutic targets for IS. We aimed to investigate the association of rs4705342 and rs4705343 polymorphisms in the promoter of miR-143/145 with risk of IS. The study population comprised 445 patients with IS and 518 controls. The rs4705342 genotype was analyzed by using a TaqMan Assay and the rs4705343 genotype was determined by using a polymerase chain reaction-restriction fragment length polymorphism assay. Relative expression of miR-143/miR-145 was measured by quantitative real-time PCR. We found that the rs4705342 was associated with a decreased risk of IS (TC vs. TT: adjusted OR = 0.74, 95% CI, 0.57–0.97; CC vs. TT: adjusted OR = 0.53, 95% CI, 0.34–0.83). Haplotype analysis showed that the TC haplotype was associated with an increased risk of IS risk (OR = 1.33, 95% CI, 1.01–1.75), whereas the CT haplotype was associated with a decreased risk of IS risk (OR = 0.68, 95% CI, 0.50–0.92). Importantly, patients carrying the rs4705342TC/CC genotypes had a lower level of miR-145 (P = 0.03). We found for the first time that the rs4705342 CC was a protective factor for IS, probably by reducing the level of miR-145. PMID:27708363

  2. Anticancer activity of rhamnoallosan against DU-145 cells is kinetically complementary to coexisting Polyphenolics in Psidium guajava budding leaves.

    Science.gov (United States)

    Chen, Kuan-Chou; Hsieh, Chiu-Lan; Huang, Kuan-Dar; Ker, Yaw-Bee; Chyau, Charng-Cherng; Peng, Robert Y

    2009-07-22

    Psidium guajava L. is a valuable farm fruit plant having many medicinal uses. Previously its budding leaves (PE) were shown to contain huge amounts of soluble polyphenolics (SP) including (in mg/g) gallic acid (348), catechin (102), epicatechin (60), rutin (100), quercetin (102), and rutin (100) and to exhibit potent anticancer activity. However, reconstitution of these polyphenolics recovered only 40% of the original bioactivity, and the soluble carbohydrate (SC) portion in PE was suspected to contribute the remaining. PE contained a novel rhamnoallosan, which had a carbohydrate/protein (w/w) ratio = 29.06%/10.27% (=2.83, average molecular mass of 5029 kDa), characteristically evidencing a peptidoglycan, consisting of a composition (mole % ratio) of rhamnose/allose/arabinose/tallose/xylose/fucose/glucose/mannose/galactose = 36.05:24.24:8.76:7.95:7.37:5.90:3.69:3.19:2.85 and of amino acid (in wt %) glycine/leucine/proline/alanine/methionine/isoleucine/valine/histidine/tyrosine/phenylalanine/cysteine/aspartic acid/lysine/glutamic acid = 37.12:12.68:10.05:8.97:5.99:4.89:4.83:4.25:4.05:2.78:1.86:1.10:0.73:0.70. Kinetic analysis showed comparable apparent cell-killing rate coefficients (k(app)) to be 4.03 x 10(3) and 2.92 x 10(3) cells mg(-1) h(-1), respectively, by SP and SC, evidencing the complementary anti-DU-145 bioactivity in nature.

  3. Crystal Structure of the Ligand Binding Suppressor Domain of Type 1 Inositol 1,4,5-Trisphosphate Receptor

    Energy Technology Data Exchange (ETDEWEB)

    Bosanac, Ivan; Yamazaki, Haruka; Matsu-ura, Toru; Michikawa, Takayuki; Mikoshiba, Katsuhiko; Ikura, Mitsuhiko (U. of Texas-SMED)

    2010-11-10

    Binding of inositol 1,4,5-trisphosphate (IP{sub 3}) to the amino-terminal region of IP{sub 3} receptor promotes Ca{sup 2+} release from the endoplasmic reticulum. Within the amino terminus, the first 220 residues directly preceding the IP{sub 3} binding core domain play a key role in IP{sub 3} binding suppression and regulatory protein interaction. Here we present a crystal structure of the suppressor domain of the mouse type 1 IP{sub 3} receptor at 1.8 {angstrom}. Displaying a shape akin to a hammer, the suppressor region contains a Head subdomain forming the {beta}-trefoil fold and an Arm subdomain possessing a helix-turn-helix structure. The conserved region on the Head subdomain appeared to interact with the IP{sub 3} binding core domain and is in close proximity to the previously proposed binding sites of Homer, RACK1, calmodulin, and CaBP1. The present study sheds light onto the mechanism underlying the receptor's sensitivity to the ligand and its communication with cellular signaling proteins.

  4. Intermolecular alignment in Y145Stop human prion protein amyloid fibrils probed by solid-state NMR spectroscopy.

    Science.gov (United States)

    Helmus, Jonathan J; Surewicz, Krystyna; Apostol, Marcin I; Surewicz, Witold K; Jaroniec, Christopher P

    2011-09-07

    The Y145Stop mutant of human prion protein, huPrP23-144, has been linked to PrP cerebral amyloid angiopathy, an inherited amyloid disease, and also serves as a valuable in vitro model for investigating the molecular basis of amyloid strains. Prior studies of huPrP23-144 amyloid by magic-angle-spinning (MAS) solid-state NMR spectroscopy revealed a compact β-rich amyloid core region near the C-terminus and an unstructured N-terminal domain. Here, with the focus on understanding the higher-order architecture of huPrP23-144 fibrils, we probed the intermolecular alignment of β-strands within the amyloid core using MAS NMR techniques and fibrils formed from equimolar mixtures of (15)N-labeled protein and (13)C-huPrP23-144 prepared with [1,3-(13)C(2)] or [2-(13)C]glycerol. Numerous intermolecular correlations involving backbone atoms observed in 2D (15)N-(13)C spectra unequivocally suggest an overall parallel in-register alignment of the β-sheet core. Additional experiments that report on intermolecular (15)N-(13)CO and (15)N-(13)Cα dipolar couplings yielded an estimated strand spacing that is within ∼10% of the distances of 4.7-4.8 Å typical for parallel β-sheets.

  5. Protein kinase A increases type-2 inositol 1,4,5-trisphosphate receptor activity by phosphorylation of serine 937.

    Science.gov (United States)

    Betzenhauser, Matthew J; Fike, Jenna L; Wagner, Larry E; Yule, David I

    2009-09-11

    Protein kinase A (PKA) phosphorylation of inositol 1,4,5-trisphosphate receptors (InsP(3)Rs) represents a mechanism for shaping intracellular Ca(2+) signals following a concomitant elevation in cAMP. Activation of PKA results in enhanced Ca(2+) release in cells that express predominantly InsP(3)R2. PKA is known to phosphorylate InsP(3)R2, but the molecular determinants of this effect are not known. We have expressed mouse InsP(3)R2 in DT40-3KO cells that are devoid of endogenous InsP(3)R and examined the effects of PKA phosphorylation on this isoform in unambiguous isolation. Activation of PKA increased Ca(2+) signals and augmented the single channel open probability of InsP(3)R2. A PKA phosphorylation site unique to the InsP(3)R2 was identified at Ser(937). The enhancing effects of PKA activation on this isoform required the phosphorylation of Ser(937), since replacing this residue with alanine eliminated the positive effects of PKA activation. These results provide a mechanism responsible for the enhanced Ca(2+) signaling following PKA activation in cells that express predominantly InsP(3)R2.

  6. Using concatenated subunits to investigate the functional consequences of heterotetrameric inositol 1,4,5-trisphosphate receptors.

    Science.gov (United States)

    Chandrasekhar, Rahul; Alzayady, Kamil J; Yule, David I

    2015-06-01

    Inositol 1,4,5-trisphosphate receptors (IP3Rs) are a family of ubiquitous, ER localized, tetrameric Ca2+ release channels. There are three subtypes of the IP3Rs (R1, R2, R3), encoded by three distinct genes, that share ∼60-70% sequence identity. The diversity of Ca2+ signals generated by IP3Rs is thought to be largely the result of differential tissue expression, intracellular localization and subtype-specific regulation of the three subtypes by various cellular factors, most significantly InsP3, Ca2+ and ATP. However, largely unexplored is the notion of additional signal diversity arising from the assembly of both homo and heterotetrameric InsP3Rs. In the present article, we review the biochemical and functional evidence supporting the existence of homo and heterotetrameric populations of InsP3Rs. In addition, we consider a strategy that utilizes genetically concatenated InsP3Rs to study the functional characteristics of heterotetramers with unequivocally defined composition. This approach reveals that the overall properties of IP3R are not necessarily simply a blend of the constituent monomers but that specific subtypes appear to dominate the overall characteristics of the tetramer. It is envisioned that the ability to generate tetramers with defined wild type and mutant subunits will be useful in probing fundamental questions relating to IP3R structure and function.

  7. Fragmented inositol 1,4,5-trisphosphate receptors retain tetrameric architecture and form functional Ca2+ release channels.

    Science.gov (United States)

    Alzayady, Kamil J; Chandrasekhar, Rahul; Yule, David I

    2013-04-19

    Inositol 1,4,5-trisphosphate receptor isoforms are a family of ubiquitously expressed ligand-gated channels encoded by three individual genes. The proteins are localized to membranes of intracellular Ca(2+) stores and play pivotal roles in Ca(2+) homeostasis. Previous studies have demonstrated that IP3R1 is cleaved by the intracellular proteases calpain and caspase both in vivo and in vitro. However, the resultant cleavage products are poorly defined, and the functional consequences of these proteolytic events are not fully understood. We demonstrate that IP3R1 is cleaved during staurosporine-induced apoptosis, yielding N-terminal fragments encompassing the ligand-binding domain and the majority of the central modulatory domain together with a C-terminal fragment containing the channel domain and cytosolic tail. Notably, these fragments remain associated with the membrane after initiation of apoptotic cleavage. Furthermore, when recombinant IP3R1 fragments, corresponding to those predicted to be generated by caspase or calpain cleavage, are stably coexpressed in cells, they physically associate and form functional channels. These data provide novel insights regarding the regulation of IP3R1 during proteolysis and provide direct evidence that polypeptide continuity is not required for IP3R activation and Ca(2+) release.

  8. The BRCA1 Tumor Suppressor Binds to Inositol 1,4,5-Trisphosphate Receptors to Stimulate Apoptotic Calcium Release*

    Science.gov (United States)

    Hedgepeth, Serena C.; Garcia, M. Iveth; Wagner, Larry E.; Rodriguez, Ana M.; Chintapalli, Sree V.; Snyder, Russell R.; Hankins, Gary D. V.; Henderson, Beric R.; Brodie, Kirsty M.; Yule, David I.; van Rossum, Damian B.; Boehning, Darren

    2015-01-01

    The inositol 1,4,5-trisphosphate receptor (IP3R) is a ubiquitously expressed endoplasmic reticulum (ER)-resident calcium channel. Calcium release mediated by IP3Rs influences many signaling pathways, including those regulating apoptosis. IP3R activity is regulated by protein-protein interactions, including binding to proto-oncogenes and tumor suppressors to regulate cell death. Here we show that the IP3R binds to the tumor suppressor BRCA1. BRCA1 binding directly sensitizes the IP3R to its ligand, IP3. BRCA1 is recruited to the ER during apoptosis in an IP3R-dependent manner, and, in addition, a pool of BRCA1 protein is constitutively associated with the ER under non-apoptotic conditions. This is likely mediated by a novel lipid binding activity of the first BRCA1 C terminus domain of BRCA1. These findings provide a mechanistic explanation by which BRCA1 can act as a proapoptotic protein. PMID:25645916

  9. Volcanic synchronisation between the EPICA Dome C and Vostok ice cores (Antarctica 0–145 kyr BP

    Directory of Open Access Journals (Sweden)

    R. Udisti

    2012-06-01

    Full Text Available This study aims at refining the synchronisation between the EPICA Dome C (EDC and Vostok ice cores in the time interval 0–145 kyr BP by using the volcanic signatures. 102 common volcanic events were identified by using continuous electrical conductivity (ECM, di-electrical profiling (DEP and sulfate measurements while trying to minimize the distortion of the glaciological chronologies. This is an update and a continuation of previous works performed over the 0–45 kyr interval that provided 56 tie points to the ice core chronologies (Udisti et al., 2004. This synchronisation will serve to establish Antarctic Ice Core Chronology 2012, the next synchronised Antarctic dating. A change of slope in the EDC-depth/Vostok-depth diagram is probably related to a change of accumulation regime as well as to a change of ice thickness upstream of the Lake Vostok, but we did not invoke any significant temporal change of surface accumulation at EDC relative to Vostok. No significant phase difference is detected between the EDC and Vostok isotopic records, but depth shifts between the Vostok 3G and 5G ice cores prevent from looking at this problem accurately. Three possible candidates for the Toba volcanic super-eruption ~73 kyr ago are suggested in the Vostok and EDC volcanic records. Neither the ECM, DEP nor the sulfate fingerprints for these 3 events are significantly larger than many others in the records.

  10. Inositol 1,4,5-trisphosphate signalling regulates the avoidance response to nose touch in Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Denise S Walker

    2009-09-01

    Full Text Available When Caenorhabditis elegans encounters an unfavourable stimulus at its anterior, it responds by initiating an avoidance response, namely reversal of locomotion. The amphid neurons, ASHL and ASHR, are polymodal in function, with roles in the avoidance responses to high osmolarity, nose touch, and both volatile and non-volatile repellents. The mechanisms that underlie the ability of the ASH neurons to respond to such a wide range of stimuli are still unclear. We demonstrate that the inositol 1,4,5-trisphosphate receptor (IP(3R, encoded by itr-1, functions in the reversal responses to nose touch and benzaldehyde, but not in other known ASH-mediated responses. We show that phospholipase Cbeta (EGL-8 and phospholipase Cgamma (PLC-3, which catalyse the production of IP(3, both function upstream of ITR-1 in the response to nose touch. We use neuron-specific gene rescue and neuron-specific disruption of protein function to show that the site of ITR-1 function is the ASH neurons. By rescuing plc-3 and egl-8 in a neuron-specific manner, we show that both are acting in ASH. Imaging of nose touch-induced Ca(2+ transients in ASH confirms these conclusions. In contrast, the response to benzaldehyde is independent of PLC function. Thus, we have identified distinct roles for the IP(3R in two specific responses mediated by ASH.

  11. Galaxy Group at z=0.3 Associated with the Damped Lyman Alpha System Towards Quasar Q1127-145

    CERN Document Server

    Kacprzak, G G; Churchill, C W

    2010-01-01

    We performed a spectroscopic galaxy survey, complete to $m_{F814W}\\leq20.3$ ($L_B>0.15L_B^{\\star}$ at z=0.3), within 100x100'' of the quasar Q1127-145 ($z_{em}=1.18$). The VLT/UVES quasar spectrum contains three $z_{abs}<0.33$ MgII absorption systems. We obtained eight new galaxy redshifts, adding to the four previously known, and galaxy star formation rates (SFRs) and metallicities were computed where possible. A strong MgII system [$W_r(2796)=1.8$A], which is a known damped Ly$\\alpha$ absorber (DLA), had three previously identified galaxies; we found two additional galaxies associated with this system. These five galaxies form a group with diverse properties, such as a luminosity range of $0.04\\leq L_B\\leq0.63 L_B^{\\star}$, an impact parameter range of $17\\leq D \\leq 241$ kpc and velocity dispersion of $\\sigma$=115 km/s. The DLA group galaxy redshifts span beyond the 350 km/s velocity spread of the metallic absorption lines of the DLA itself. The two brightest group galaxies have SFRs of $\\sim$few $M_{\\o...

  12. 1.45 Å resolution structure of SRPN18 from the malaria vector Anopheles gambiae

    Energy Technology Data Exchange (ETDEWEB)

    Meekins, David A.; Zhang, Xin; Battaile, Kevin P.; Lovell, Scott; Michel, Kristin (Kansas); (KSU); (HWMRI)

    2016-11-19

    Serine protease inhibitors (serpins) in insects function within development, wound healing and immunity. The genome of the African malaria vector,Anopheles gambiae, encodes 23 distinct serpin proteins, several of which are implicated in disease-relevant physiological responses.A. gambiaeserpin 18 (SRPN18) was previously categorized as non-inhibitory based on the sequence of its reactive-center loop (RCL), a region responsible for targeting and initiating protease inhibition. The crystal structure ofA. gambiaeSRPN18 was determined to a resolution of 1.45 Å, including nearly the entire RCL in one of the two molecules in the asymmetric unit. The structure reveals that the SRPN18 RCL is extremely short and constricted, a feature associated with noncanonical inhibitors or non-inhibitory serpin superfamily members. Furthermore, the SRPN18 RCL does not contain a suitable protease target site and contains a large number of prolines. The SRPN18 structure therefore reveals a unique RCL architecture among the highly conserved serpin fold.

  13. Inositol 1,4,5-trisphosphate 3-kinase B controls survival and prevents anergy in B cells.

    Science.gov (United States)

    Maréchal, Yoann; Quéant, Séverine; Polizzi, Selena; Pouillon, Valérie; Schurmans, Stéphane

    2011-01-01

    Inositol 1,4,5-trisphosphate 3-kinase B (or Itpkb) and inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), its reaction product, play an important role in the control of B lymphocyte fate and function in vivo. In order to investigate the fine mechanisms of Itpkb and Ins(1,3,4,5)P4 action in B cells, we crossed Itpkb(-/-) mice with transgenic mice expressing a 3-83μδ B cell receptor (BCR) specific for membrane-bound MHC-I H2-K(b) and H2-K(k) molecules. On a non-deleting H2-K(d) genetic background, we show that Itpkb is important for the control of Bim protein expression and B cell survival rather than for the control of B cell development from one stage to another. Analyses of cell surface markers expression, proapoptotic Bim protein expression, in vitro survival and in vivo turnover demonstrated that BCR transgenic Itpkb(-/-) B cells exhibit an anergic phenotype with the notable exception of their enhanced antigen-induced calcium signalling. On a deleting H2-K(b) genetic background, we show that Itpkb is not essential for BCR editing or negative selection. These data establish Itpkb as an important regulator of B cell survival and anergy in vivo.

  14. Volcanic synchronisation between the EPICA Dome C and Vostok ice cores (Antarctica 0–145 kyr BP

    Directory of Open Access Journals (Sweden)

    F. Parrenin

    2011-12-01

    Full Text Available This study aims at refining the synchronisation between the EPICA Dome C (EDC and Vostok ice cores in the time interval 0–145 kyr BP by using the volcanic signatures. 111 common volcanic events were identified by using continuous electrical conductivity (ECM, di-electrical profiling (DEP and sulfate measurements while trying to minimize the distortion of the glaciological chronologies. This is an update and a continuation of previous works performed over the 0–45 kyr interval which provided 56 tie points to the ice core chronologies (Udisti et al., 2004. This synchronisation will serve for the establishment of the next synchronised Antarctic dating. A change of slope in the EDC-depth/Vostok-depth diagram is probably related to a change of accumulation regime as well as to a change of ice thickness upstream of the Vostok lake, but we did not invoke any significant temporal change of surface accumulation at EDC relative to Vostok. A significant phase difference is detected between the EDC and Vostok isotopic records during the 95–120 kyr interval, but not during Termination II. Three possible candidates for the Toba volcanic super-eruption ~73 kyr ago are suggested in the Vostok and EDC volcanic records. However the ECM, DEP and sulfate fingerprints for these three events are not significantly larger than many others in the records.

  15. Antisense Oligonucleotides Targeting Parasite Inositol 1,4,5-Trisphosphate Receptor Inhibits Mammalian Host Cell Invasion by Trypanosoma cruzi

    Science.gov (United States)

    Hashimoto, Muneaki; Nara, Takeshi; Hirawake, Hiroko; Morales, Jorge; Enomoto, Masahiro; Mikoshiba, Katsuhiko

    2014-02-01

    Chagas disease is caused by an intracellular parasitic protist, Trypanosoma cruzi. As there are no highly effective drugs against this agent that also demonstrate low toxicity, there is an urgent need for development of new drugs to treat Chagas disease. We have previously demonstrated that the parasite inositol 1,4,5-trisphosphate receptor (TcIP3R) is crucial for invasion of the mammalian host cell by T. cruzi. Here, we report that TcIP3R is a short-lived protein and that its expression is significantly suppressed in trypomastigotes. Treatment of trypomastigotes, an infective stage of T. cruzi, with antisense oligonucleotides specific to TcIP3R deceased TcIP3R protein levels and impaired trypomastigote invasion of host cells. Due to the resulting instability and very low expression level of TcIP3R in trypomastigotes indicates that TcIP3R is a promising target for antisense therapy in Chagas disease.

  16. Non-destructive evaluation of scientific and biological samples by scattering of 145 keV gamma rays

    Energy Technology Data Exchange (ETDEWEB)

    Singh, M.P.; Sharma, Amandeep; Singh, Bhajan [Physics Department, Punjabi University, Patiala, Punjab 147002 (India); Sandhu, B.S., E-mail: balvir@pbi.ac.i [Physics Department, Punjabi University, Patiala, Punjab 147002 (India)

    2010-09-15

    The objective of present experiment is to assign effective atomic number (Z{sub eff}) to samples of scientific interest (oxides of lanthanoids, also called rare earths, and alloys of lead and tin of known composition) and to measure stable iodine content of tissue (biological sample). An HPGe semiconductor detector, placed at 70{sup o} to the incident beam, detects gamma photons scattered from the sample under investigation. The experiment is performed on various elements with atomic number satisfying, 6 {<=}Z {<=} 82, for 145 keV incident photons. The intensity ratio of Rayleigh to Compton scattered peaks, corrected for photo-peak efficiency of gamma detector and absorption of photons in the sample and air, is plotted as a function of atomic number and constituted a fit curve. From this fit curve, the respective effective atomic numbers of the scientific samples are determined. The agreement of measured values of Z{sub eff} with theoretical calculations is found to be quite satisfactory. The measured intensity ratio from phantom (KI solutions, simulating thyroid content of stable iodine) varies linearly with KI concentration and provides stable iodine content of tissue.

  17. Material composition assessment and discovering sublimation activity on asteroids 145 Adeona, 704 Interamnia, 779 Nina, and 1474 Beira

    Science.gov (United States)

    Busarev, V. V.; Barabanov, S. I.; Puzin, V. B.

    2016-07-01

    Spectrophotometric observations of 145 Adeona, 704 Interamnia, 779 Nina, and 1474 Beira—asteroids of close primitive types—allowed us to detect similar mineralogical absorption bands in their reflectance spectra centered in the range 0.35 to 0.92 μm; the bands are at 0.38, 0.44, and 0.67-0.71 μm. On the same asteroids, the spectral signs of simultaneous sublimation activity were found for the first time. Namely, there are maxima at ˜0.35-0.60 μm in the reflectance spectra of Adeona, Interamnia, and Nina and at ˜0.55-075 μm in the spectra of Beira. We connect this activity with small heliocentric distances of the asteroids and, consequently, with a high insolation at their surfaces. Examination of the samples of probable analogues allowed us to identify Fe3+ and Fe2+ in the material of these asteroids through the mentioned absorption bands. For analogues, we took powdered samples of carbonaceous chondrites Orgueil (CI), Mighei (CM2), Murchison (CM2), and Boriskino (CM2), as well as hydrosilicates of the serpentine group. Laboratory spectral reflectance study of the samples of low-iron Mg serpentines (heliocentric distances, we may suppose that this phenomenon is common for the main-belt asteroids.

  18. A Phase 3 Study of Evolocumab (AMG 145) in Statin-Treated Japanese Patients at High Cardiovascular Risk.

    Science.gov (United States)

    Kiyosue, Arihiro; Honarpour, Narimon; Kurtz, Christopher; Xue, Allen; Wasserman, Scott M; Hirayama, Atsushi

    2016-01-01

    Evolocumab (AMG 145), a fully human monoclonal antibody against PCSK9, significantly reduced low-density lipoprotein cholesterol (LDL-C) levels in phase 2 and 3 studies. This phase 3 study evaluated the efficacy and safety of evolocumab plus atorvastatin in Japanese patients with hyperlipidemia or mixed dyslipidemia and high cardiovascular risk. Patients were randomized to atorvastatin 5 or 20 mg/day for 4 weeks. Subsequently, patients underwent second randomization to evolocumab 140 mg biweekly (Q2W) or 420 mg monthly (QM) or placebo Q2W or QM. Coprimary end points were % change from baseline in LDL-C at week 12 and mean of weeks 10 and 12. Secondary end points included change and % change in other lipids and proportion of patients reaching LDL-C evolocumab); 39% and 40% were women; 14% and 12% had cerebrovascular or peripheral arterial disease; and 51% and 47% had diabetes. At entry, mean (SD) calculated LDL-C was 128 (23) mg/dL; after stabilization on atorvastatin 5 and 20 mg/day, baseline LDL-C levels were 118 (35) and 94 (24) mg/dL, respectively. Mean LDL-C reductions at week 12 for evolocumab versus placebo ranged from 67% to 76%. No imbalances were observed in adverse events between treatment groups. Efficacy and safety for Q2W or QM evolocumab dosing were similar. In conclusion, in high-risk Japanese patients receiving stable statin therapy, evolocumab markedly reduced LDL-C and was well tolerated.

  19. Mesozoic fossils (>145 Mya) suggest the antiquity of the subgenera of Daphnia and their coevolution with chaoborid predators

    Science.gov (United States)

    2011-01-01

    Background The timescale of the origins of Daphnia O. F. Mueller (Crustacea: Cladocera) remains controversial. The origin of the two main subgenera has been associated with the breakup of the supercontinent Pangaea. This vicariance hypothesis is supported by reciprocal monophyly, present day associations with the former Gondwanaland and Laurasia regions, and mitochondrial DNA divergence estimates. However, previous multilocus nuclear DNA sequence divergence estimates at Daphnia. Results We describe new fossils of ephippia from the Khotont site in Mongolia associated with the Jurassic-Cretaceous boundary (about 145 MYA) that are morphologically similar to several modern genera of the family Daphniidae, including the two major subgenera of Daphnia, i.e., Daphnia s. str. and Ctenodaphnia. The daphniid fossils co-occurred with fossils of the predaceous phantom midge (Chaoboridae). Conclusions Our findings indicate that the main subgenera of Daphnia are likely much older than previously known from fossils (at least 100 MY older) or from nuclear DNA estimates of divergence. The results showing co-occurrence of the main subgenera far from the presumed Laurasia/Gondwanaland dispersal barrier shortly after formation suggests that vicariance from the breakup of Pangaea is an unlikely explanation for the origin of the main subgenera. The fossil impressions also reveal that the coevolution of a dipteran predator (Chaoboridae) with the subgenus Daphnia is much older than previously known -- since the Mesozoic. PMID:21595889

  20. Interactions between the inositol 1,4,5-trisphosphate and cyclic AMP signaling pathways regulate larval molting in Drosophila.

    Science.gov (United States)

    Venkatesh, K; Siddhartha, G; Joshi, R; Patel, S; Hasan, G

    2001-05-01

    Larval molting in Drosophila, as in other insects, is initiated by the coordinated release of the steroid hormone ecdysone, in response to neural signals, at precise stages during development. In this study we have analyzed, using genetic and molecular methods, the roles played by two major signaling pathways in the regulation of larval molting in Drosophila. Previous studies have shown that mutants for the inositol 1,4,5-trisphosphate receptor gene (itpr) are larval lethals. In addition they exhibit delays in molting that can be rescued by exogenous feeding of 20-hydroxyecdysone. Here we show that mutants for adenylate cyclase (rut) synergize, during larval molting, with itpr mutant alleles, indicating that both cAMP and InsP(3) signaling pathways function in this process. The two pathways act in parallel to affect molting, as judged by phenotypes obtained through expression of dominant negative and dominant active forms of protein kinase A (PKA) in tissues that normally express the InsP(3) receptor. Furthermore, our studies predict the existence of feedback inhibition through protein kinase A on the InsP(3) receptor by increased levels of 20-hydroxyecdysone.

  1. Temperature-dependence and conformational basis of inositol 1,4,5-trisphosphate receptor regulated by Ca2+

    Institute of Scientific and Technical Information of China (English)

    胡广; 黄有国; 杨福愉

    2000-01-01

    The inositol 1,4,5-trisphosphate (lnsP3) receptor was purified from bovine cerebellum and reconstituted in liposomes composed of phosphatidylcholine (PC) and phosphatidylethanola-mine (PE) (1:1) successfully. No effect of Ca2+ concentration on [3H]-lnsP3 binding to unreconsti-tuted lnsP3 receptor could be observed either at 4℃ or at 25℃, whereas the effect of [Ca2+] on reconstituted lnsP3 receptor depended on the temperature. The Ca2+ concentration outside the proteolipsome ([Ca2+]o) had no detectable effect on lnsP3 binding to lnsP3 receptor at 4℃. In contrast, with increase of [Ca2+]o from 0 to 100 nmol/L at 25℃, the lnsP3 binding activity increased gradually. Then the lnsP3 binding activity was decreased drastically at higher [Ca2+]0 and inhibited entirely at 50 nmol/L [Ca2+]. Conformational studies on intrinsic fluorescence of the reconstituted lnsP3 receptor and its quenching by Kl and HB indicated that the global conformation of reconstituted lnsP3 receptor could not be affected by [Ca2+]o at

  2. Aberrant calcium signaling by transglutaminase-mediated posttranslational modification of inositol 1,4,5-trisphosphate receptors.

    Science.gov (United States)

    Hamada, Kozo; Terauchi, Akiko; Nakamura, Kyoko; Higo, Takayasu; Nukina, Nobuyuki; Matsumoto, Nagisa; Hisatsune, Chihiro; Nakamura, Takeshi; Mikoshiba, Katsuhiko

    2014-09-23

    The inositol 1,4,5-trisphosphate receptor (IP3R) in the endoplasmic reticulum mediates calcium signaling that impinges on intracellular processes. IP3Rs are allosteric proteins comprising four subunits that form an ion channel activated by binding of IP3 at a distance. Defective allostery in IP3R is considered crucial to cellular dysfunction, but the specific mechanism remains unknown. Here we demonstrate that a pleiotropic enzyme transglutaminase type 2 targets the allosteric coupling domain of IP3R type 1 (IP3R1) and negatively regulates IP3R1-mediated calcium signaling and autophagy by locking the subunit configurations. The control point of this regulation is the covalent posttranslational modification of the Gln2746 residue that transglutaminase type 2 tethers to the adjacent subunit. Modification of Gln2746 and IP3R1 function was observed in Huntington disease models, suggesting a pathological role of this modification in the neurodegenerative disease. Our study reveals that cellular signaling is regulated by a new mode of posttranslational modification that chronically and enzymatically blocks allosteric changes in the ligand-gated channels that relate to disease states.

  3. Barge loading facilities in conjunction with wood chipping and sawlog mill, Tennessee River Mile 145. 9R: Environmental assessment

    Energy Technology Data Exchange (ETDEWEB)

    1990-08-01

    The purpose of this Environmental Assessment (EA) is to evaluate the environmental consequences of approving, denying, or adopting reasonable alternatives to a request for barge loading facilities. These facilities would serve a proposed wood chipping and sawlog products operation at Tennessee River Mile (TRM) 145.9, right descending bank, (Kentucky Lake), in Perry County, Tennessee. The site is located between Short Creek and Peters Landing. The applicant is Southeastern Forest Products, L.P. (SFP), Box 73, Linden, Tennessee and the proposed facilities would be constructed on or adjacent to company owned land. Portions of the barge terminal would be constructed on land over which flood easement rights are held by the United States of America and administered by the Tennessee Valley Authority (TVA). The US Army Corps of Engineers (CE) and TVA have regulatory control over the proposed barge terminal facilities since the action would involve construction in the Tennessee River which is a navigable water of the United States. The wood chipping and sawlog products facilities proposed on the upland property are not regulated by the CE or TVA. On the basis of the analysis which follows, it has been determined that a modified proposal (as described herein) would not significantly affect the quality of the human environment, and does not require the preparation of an environmental impact statement. 8 refs.

  4. Mesozoic fossils (>145 Mya suggest the antiquity of the subgenera of Daphnia and their coevolution with chaoborid predators

    Directory of Open Access Journals (Sweden)

    Taylor Derek J

    2011-05-01

    Full Text Available Abstract Background The timescale of the origins of Daphnia O. F. Mueller (Crustacea: Cladocera remains controversial. The origin of the two main subgenera has been associated with the breakup of the supercontinent Pangaea. This vicariance hypothesis is supported by reciprocal monophyly, present day associations with the former Gondwanaland and Laurasia regions, and mitochondrial DNA divergence estimates. However, previous multilocus nuclear DNA sequence divergence estimates at Daphnia. Results We describe new fossils of ephippia from the Khotont site in Mongolia associated with the Jurassic-Cretaceous boundary (about 145 MYA that are morphologically similar to several modern genera of the family Daphniidae, including the two major subgenera of Daphnia, i.e., Daphnia s. str. and Ctenodaphnia. The daphniid fossils co-occurred with fossils of the predaceous phantom midge (Chaoboridae. Conclusions Our findings indicate that the main subgenera of Daphnia are likely much older than previously known from fossils (at least 100 MY older or from nuclear DNA estimates of divergence. The results showing co-occurrence of the main subgenera far from the presumed Laurasia/Gondwanaland dispersal barrier shortly after formation suggests that vicariance from the breakup of Pangaea is an unlikely explanation for the origin of the main subgenera. The fossil impressions also reveal that the coevolution of a dipteran predator (Chaoboridae with the subgenus Daphnia is much older than previously known -- since the Mesozoic.

  5. Diverse Phenotypic Expression of Cardiomyopathies in a Family with TNNI3 p.Arg145Trp Mutation

    Science.gov (United States)

    Hwang, Ji-won; Jang, Mi-Ae; Jang, Shin Yi; Seo, Soo Hyun; Seong, Moon-Woo; Park, Sung Sup; Ki, Chang-Seok

    2017-01-01

    Genetic diagnosis of cardiomyopathies is challenging, due to the marked genetic and allelic heterogeneity and the lack of knowledge of the mutations that lead to clinical phenotypes. Here, we present the case of a large family, in which a single TNNI3 mutation caused variable phenotypic expression, ranging from restrictive cardiomyopathy (RCMP) to hypertrophic cardiomyopathy (HCMP) to near-normal phenotype. The proband was a 57-year-old female with HCMP. Examining the family history revealed that her elder sister had expired due to severe RCMP. Using a next-generation sequencing-based gene panel to analyze the proband, we identified a known TNNI3 gene mutation, c.433C>T, which is predicted to cause an amino acid substitution (p.Arg145Trp) in the highly conserved inhibitory region of the cardiac troponin I protein. Sanger sequencing confirmed that six relatives with RCMP or near-normal phenotypes also carried this mutation. To our knowledge, this is the first genetically confirmed family with diverse phenotypic expression of cardiomyopathies in Korea. Our findings demonstrate familial implications, where a single mutation in a sarcomere protein can cause diverse phenotypic expression of cardiomyopathies.

  6. X-ray diffraction studies of 145MeV proton-irradiated AlBeMet 162

    Directory of Open Access Journals (Sweden)

    Mohamed Elbakhshwan

    2016-08-01

    Full Text Available AlBeMet 162 (Materion Co., formerly Brush Wellman has been irradiated with 145MeV protons up to 1.2×1020cm−2 fluence, with irradiation temperatures in the range of 100–220°C. Macroscopic post-irradiation evaluation on the evolution of mechanical and thermal properties was integrated with a comprehensive X-ray- diffraction study using high-energy monochromatic and polychromatic X-ray beams, which offered a microscopic view of the irradiation damage effects on AlBeMet. The study confirmed the stability of the metal–matrix composite, its resistance to proton damage, and the continuing separation of the two distinct phases, fcc aluminum and hcp beryllium, following irradiation. Furthermore, based on the absence of inter-planar distance change during proton irradiation, it was confirmed that the stacking faults and clusters on the Al (111 planes are stable, and thus can migrate from the cascade region and be absorbed at various sinks. XRD analysis of the unirradiated AlBeMet 162 showed clear change in the texture of the fcc phase with orientation especially in the Al (111 reflection which exhibits a “non-perfect” six-fold symmetry, implying lack of isotropy in the composite.

  7. miRNA -145在子宫内膜异位症中的诊断价值及其机制研究%Study on the diagnostic value of miRNA-145 in patients with endometriosis and its mechanism

    Institute of Scientific and Technical Information of China (English)

    郭红艳; 洛若愚; 王华

    2016-01-01

    Objective To investigate the diagnostic value of miRNA - 145 in patients with endometriosis and its possible mechanism. Methods RT - PCR was used to detect the relative expression level of miRNA - 145 in serum and endometrial tissues of patients with EMs in dif-ferent r - AFS stage. Its correlation was analyzed and the target gene was predicted. Election year because of infertility(tubal factor)patients and mesosalpinx cyst surgery of 27 cases as the control group. Results The relative expression levels of tissue miRNA - 145 in Ectopic group and Eu-topic group were higher than those in control group( P < 0. 05). The relative expression of serum miR - 145 in EMs1,EMs2,EMs3 and EMs4 were higher than that in control group( P < 0. 05),and serum miRNA - 145 in the VAS1,VAS2,VAS3 and VAS4 group were higher than those in control group( P < 0. 05). The target gene prediction results indicated that there were 5 genes related to EMs:CRK、PTEN,VEGFA,PGR and MMP9. Conclusion The specific relative expression of miRNA - 145 may be used as a marker for early diagnosis of EMs,and is a molecular marker for the diagnosis and prognosis of EMs for the future.%目的:探讨 miRNA -145在子宫内膜异位症(EMs)中的诊断价值及其可能的机制。方法采用实时荧光定量(RT - PCR)法检测不同病程阶段 EMs 患者血清及内膜组织中的 miRNA -145表达水平,分析其相关性,并预测其靶基因。选同期因不孕症(输卵管因素)及输卵管系膜囊肿等手术的患者27例作为对照组。结果 RT - PCR 检测结果显示,Ectopic 组和 Eutopic 组内膜组织 miRNA -145相对表达水平均高于对照组( P <0.05),EMs1、EMs2、EMs3和EMs4组患者血清 miRNA -145相对表达量均高于对照组( P <0.05),无疼痛组、轻度疼痛组、中度疼痛组和重度疼痛组患者血清 miRNA -145相对表达水平均高于对照组( P <0.05),且随着 r - AFS 分期进展及 VAS

  8. 龙葵碱对前列腺癌LNCaP及Du145细胞系的作用及机制%Effect and mechanism research of solanine on prostate cancer cell LNCaP and Du145

    Institute of Scientific and Technical Information of China (English)

    李志雄; 梁蔚波; 唐晖; 陈炫; 刘玉峰; 苏泽轩

    2013-01-01

    目的 探讨龙葵碱对雄激素依赖型人前列腺癌LNCaP细胞及雄激素非依赖型人前列腺癌Du145细胞的作用及其机制.方法 应用MTT法检测龙葵碱对两种细胞增殖的影响,流式细胞仪分析龙葵碱对两种细胞细胞周期的影响及细胞凋亡的情况,荧光显微镜原位观察细胞凋亡图像,Western blot法检测龙葵碱对细胞内Bcl-2、Bax、Caspase-3蛋白量表达的影响.结果 龙葵碱能显著抑制两种细胞的增殖,且呈时间与剂量依赖性(P<0.05);能使两种细胞的细胞周期阻滞在S期;各浓度龙葵碱处理组细胞凋亡比例高于0 μg/mL组且荧光显微镜下可见细胞早期凋亡形态图像;龙葵碱能下调两种细胞的Bcl-2蛋白及 Caspase-3蛋白的表达,而保持Bax蛋白的表达不变.结论 龙葵碱可以通过线粒体途径诱导Du145及LNCaP细胞凋亡的发生,能将两种细胞的细胞周期阻滞在S期,产生抑制细胞增殖的作用.%Objective To investigate the role of solanine in human androgen - dependent prostate cancer cell line LNCaP and androgen - independent prostate cancer cell Dul45. Methods The influence on the proliferation of LNCaP and Dul45 cells were evaluated by MTT assay. Cell cycle and cell apoptosis of LNCaP and Dul45 were assessed by flow cytometry and fluorescence microscope. The protein of expressions of Bel -2, Bax, and Caspase -3 were determined by Western blot. Results Solanine could significantly inhibit the proliferation of the two cells in time - and dose - dependent manners (P <0. 05) ; also induced S - phase arrest of LNCaP and Dul45 cells. The apoptosis ratio in treatment groups were significantly higher than that in 0μg/mL group, with early apoptosis. Significant down - regulation of Bel - 2 and Caspase - 3 was induced by solanine, with no effect on Bax protein. Conclusion Solanine provides anti - prostate cancer effect by inhibiting cell proliferation, S -phase arresting, and inducing apoptosis.

  9. 145例痉挛型小儿脑性瘫痪危险因素研究%Risk factors related to infantile spastic cerebral palsy among 145 cases

    Institute of Scientific and Technical Information of China (English)

    周宪君; 邱洪斌; 徐辉; 祝丽玲

    2013-01-01

    Objective To study the risk factors related to spastic type of cerebral palsy through a screening program.Methods With a 1 ∶ 2 matched case-control study design,145 cases were selected from the Cerebral Palsy Treatment and Management Center in Heilongjiang Province from Dec.,2007 through May 2009.Another 290 cases of children as control group were chosen from the First Affiliated Hospital of Jiamusi University,in the same period.Controls were excluded the central nervous system diseases,matching with cases at the age differences no more than 3 months,with same sex and ethnicity.Questionnaire survey was used to access relevant information,while logistic regression analysis was used to screen the risk factors.Results Data from Single factor logistic regression analysis notified 39 items as statistically significant factors.Through multivariate logistic regression analysis,11 factors in all the cerebral palsy cases,and incidence of cerebral palsy risk factors related to low or high birth weight were discovered as follows:mother with college degree,newborns with high muscle tone,skin color as purple,taking antipyretic analgesics drugs during pregnancy,experience significant events during pregnancy,situation of environment pollution around the house,having intracranial hemorrhage and birth asphyxia,fetal distress during pregnancy or neonatal stage etc.Conclusion Pre/Nconatal factors were closely related to cerebral palsy,suggesting that health care knowledge be popularized and related management be strengthened,to reduce the cerebral palsy incidence in the newborns.%目的 筛查痉挛型脑性瘫痪(脑瘫)的危险因素.方法 采用1∶2病例对照研究,选择2007年12月至2009年5月在黑龙江省小儿脑性瘫痪防治疗育中心就诊的脑瘫患儿145例为病例组,同时按照年龄±3个月及性别、民族相同原则进行匹配,选择同期在佳木斯大学附属第一医院就诊并排除中枢神经系统疾病的290名儿童作为对照组,

  10. 145 - 155 Abdulrahman

    African Journals Online (AJOL)

    User

    2015-12-02

    Dec 2, 2015 ... environmental changes that may lead to appearance ... irrigation farming, fishing and human activities are taking place with mean depth of ..... Diversity Indices/ Season ... collection and influence of season (Adeniji, 1993).

  11. 145 ORIGINAL ARTICLE

    African Journals Online (AJOL)

    boaz

    neuropathic bladder, increased adherence of bacterial .... ciprofloxacin (27.0%) followed by low level resistance .... treatment adherence once diagnosed. ..... obesity: targets and therapy. 2015;8:129.https://www.ncbi.nlm.nih.gov/pubme.

  12. 人食管鳞状细胞癌细胞株hsa-miR-143/145基因簇表达生物学信息的分析%Expression of hsa-miR-143/145 cluster in human ESCC cell line and bioinformatics analysis

    Institute of Scientific and Technical Information of China (English)

    蒋森; 杨晓娣; 王秀芳; 鲍子超; 付海龙; 杜云翔; 赵亚萍

    2012-01-01

    目的:探讨hsa-miR-143/145基因簇在人食管鳞状细胞癌(ESCC)细胞株中的表达,并对其生物学特征以及功能进行预测分析.方法:通过实时荧光定量PCR法,检测人ESCC细胞株KYSE-150和正常食管上皮细胞株Het-1A中hsa-miR-143/145基因簇的相对含量;应用miRBase获取并分析hsa-miR-143/145基因簇序列特征并比较其同源性大小;应用TargetScan 6.1、PicTar及miRanda预测hsa-miR-143/145基因簇的靶基因,取三者预测结果的交集,并结合已证实的靶基因进行功能注释(GO)和通路富集分析.结果:人ESCC细胞株KYSE-150中hsa-miR-143表达水平为0.000 2±0.000 5,显著低于正常食管上皮细胞Het-1A的0.000 6±0.000 7,t=10.301,P=0.001;hsa-miR-145的表达水平为0.000 5±0.000 1,显著低于正常食管上皮细胞Het-1A的0.001 2±0.000 2,t=6.139,P=0.004.应用3种靶标预测软件预测hsa-miR-143和hsa-miR-145的靶基因,取交集有119个基因,结合已证实的靶标基因,共224个.hsa-miR-143/145基因簇在21个物种上都显示了很好的同源性,其靶基因显著富集在癌症通路、与癌症发生相关的信号通路以及心肌通路中.结论:Hsa-miR-143/145基因簇可能参与ESCC的发病机制,其预测靶基因集合富集于多个生物学过程并且显著富集于癌症通路,为后续Hsa-miR-143/145基因簇生物学功能的研究奠定了基础.%OBJECTIVE:To investigate the expression of hsa-miR-143/145 cluster in human ESCC cell line,and predict its biological character and function. METHODS: The expression of hsa-miR-143/145 cluster was assessed by real time quantitative polymerase chain reaction (qPCR). MiRBase database was applied to retrieval the sequence of hsa-miR-143/145 cluster and its sequence homology. The target genes of hsa-miR-143/145 cluster were predicted using TargetScan6. l,PicTar and mi-Randa,and the intersection of the three results as gene set was analyzed by gene ontology and pathway analysis. RESULTS: The expression

  13. Extracellular Disposal of Tumor-Suppressor miRs-145 and -34a via Microvesicles and 5-FU Resistance of Human Colon Cancer Cells

    Directory of Open Access Journals (Sweden)

    Yukihiro Akao

    2014-01-01

    Full Text Available The dysregulation of microRNA (miRNA expression causes various kinds of diseases. Especially, alterations in miRNA expression levels are frequently observed in human tumor cells and are associated with cancer pathogenesis. Earlier we established Fluorouracil (5-FU-resistant human colon cancer DLD-1 cells (DLD-1/5FU from parental 5-FU- sensitive DLD-1 cells. In the present study, we examined the expression of miRNA in each cell line and in its extracellular microvesicles (MVs before and after treatment with 5-FU. The nascent RNAs of anti-oncogenic miR-34a and -145 labeled with EU in both cells were proved to be transferred into MVs in both cell lines. The levels of miR-34a and -145 in the cells and in their MVs were not largely different in the two cell lines, and a substantial amount of both miRNAs was secreted by both cell lines even in the steady-state condition. The exposure of both cell lines to 5-FU significantly increased the intracellular levels of miR-145 and miR-34a in the 5-FU-sensitive DLD-1 cells, whereas the level of neither miR was elevated in the DLD-1/5FU cells. Interestingly, the amount of miR-145 detected in the small MVs shed into the medium of the parental cells was reduced after the treatment with 5-FU. On the other hand, the intracellular expression of miR-34a in the DLD-1/5FU cells was down-regulated compared with that in the parental DLD-1 cells even in the steady-state condition. As to the miR-34a secreted into MVs, the increase in the level in DLD-1/5FU cells was greater than that in the parental DLD-1 cells after the treatment with 5-FU. Thus, the intra- and extracellular miR-145 and -34a were closely associated with 5-FU resistance, and the resistance was in part due to the enhanced secretion of miR-145 and -34a via MVs, resulting in low intracellular levels of both miRNAs.

  14. Impacts of the G145R Mutation on the Structure and Immunogenic Activity of the Hepatitis B Surface Antigen: A Computational Analysis

    Directory of Open Access Journals (Sweden)

    Rezaee

    2016-06-01

    Full Text Available Background Vaccine-escaped hepatitis B virus (HBV mutations occur within the “a” determinant area, which is located in the major hydrophilic region (MHR of the hepatitis B surface antigen (HBsAg protein. It is now well established that the common G145R mutation is highly capable of escaping from HBsAg immune recognition. However, the impacts of this mutation on the structure and immunogenic activity of HBsAg have been poorly investigated. Objectives The present study analyzed the effects of the G145R mutation on the structure and immunogenic activity of the HBsAg. Materials and Methods Three-dimensional (3D structure of HBsAg for both the wild-type and G145R mutant were predicted and refined using several web tools. After quantitative evaluations, the effects of the G145R mutation on the secondary and 3D structures of the HBsAg were investigated. In parallel, the immunogenic activity of the wild-type and mutant HBsAg was also analyzed using a ClusPro docking server as well as the IEDB web tool. Further analyses were performed via molecular dynamics (MD simulations using the GROMACS v5.0.2 simulation package. Results The G145R mutation causes a considerable reduction in the immunogenic activity of the HBsAg through a conformational change in the HBsAg antigenic loops. This mutation inserts a new β-strand in the “a” determinant region of the HBsAg, leading to a reduced binding affinity to its monoclonal antibody, MAb12. The G145R mutation also increased the compactness and stability of the HBsAg by enhancing the rigidity of the “a” determinant. Conclusions These data will be beneficial for designing more advanced antibodies for the recognition of the HBsAg in diagnostics. In addition, the results of this study may assist in the design or development of more effective hepatitis B vaccines.

  15. Defining the stoichiometry of inositol 1,4,5-trisphosphate binding required to initiate Ca2+ release.

    Science.gov (United States)

    Alzayady, Kamil J; Wang, Liwei; Chandrasekhar, Rahul; Wagner, Larry E; Van Petegem, Filip; Yule, David I

    2016-04-05

    Inositol 1,4,5-trisphosphate (IP3) receptors (IP3Rs) are tetrameric intracellular Ca(2+)-release channels with each subunit containing a binding site for IP3in the amino terminus. We provide evidence that four IP3molecules are required to activate the channel under diverse conditions. Comparing the concentration-response relationship for binding and Ca(2+)release suggested that IP3Rs are maximally occupied by IP3before substantial Ca(2+)release occurs. We showed that ligand binding-deficient subunits acted in a dominant-negative manner when coexpressed with wild-type monomers in the chicken immune cell line DT40-3KO, which lacks all three genes encoding IP3R subunits, and confirmed the same effect in an IP3R-null human cell line (HEK-3KO) generated by CRISPR/Cas9 technology. Using dimeric and tetrameric concatenated IP3Rs with increasing numbers of binding-deficient subunits, we addressed the obligate ligand stoichiometry. The concatenated IP3Rs with four ligand-binding sites exhibited Ca(2+)release and electrophysiological properties of native IP3Rs. However, IP3failed to activate IP3Rs assembled from concatenated dimers consisting of one binding-competent and one binding-deficient mutant subunit. Similarly, IP3Rs containing two monomers of IP3R2short, an IP3binding-deficient splice variant, were nonfunctional. Concatenated tetramers containing only three binding-competent ligand-binding sites were nonfunctional under a wide range of activating conditions. These data provide definitive evidence that IP3-induced Ca(2+)release only occurs when each IP3R monomer within the tetramer is occupied by IP3, thereby ensuring fidelity of Ca(2+)release.

  16. Unique Regulatory Properties of Heterotetrameric Inositol 1,4,5-Trisphosphate Receptors Revealed by Studying Concatenated Receptor Constructs.

    Science.gov (United States)

    Chandrasekhar, Rahul; Alzayady, Kamil J; Wagner, Larry E; Yule, David I

    2016-03-01

    The ability of inositol 1,4,5-trisphosphate receptors (IP3R) to precisely initiate and generate a diverse variety of intracellular Ca(2+) signals is in part mediated by the differential regulation of the three subtypes (R1, R2, and R3) by key functional modulators (IP3, Ca(2+), and ATP). However, the contribution of IP3R heterotetramerization to Ca(2+) signal diversity has largely been unexplored. In this report, we provide the first definitive biochemical evidence of endogenous heterotetramer formation. Additionally, we examine the contribution of individual subtypes within defined concatenated heterotetramers to the shaping of Ca(2+) signals. Under conditions where key regulators of IP3R function are optimal for Ca(2+) release, we demonstrate that individual monomers within heteromeric IP3Rs contributed equally toward generating a distinct 'blended' sensitivity to IP3 that is likely dictated by the unique IP3 binding affinity of the heteromers. However, under suboptimal conditions where [ATP] were varied, we found that one subtype dictated the ATP regulatory properties of heteromers. We show that R2 monomers within a heterotetramer were both necessary and sufficient to dictate the ATP regulatory properties. Finally, the ATP-binding site B in R2 critical for ATP regulation was mutated and rendered non-functional to address questions relating to the stoichiometry of IP3R regulation. Two intact R2 monomers were sufficient to maintain ATP regulation in R2 homotetramers. In summary, we demonstrate that heterotetrameric IP3R do not necessarily behave as the sum of the constituent subunits, and these properties likely extend the versatility of IP3-induced Ca(2+) signaling in cells expressing multiple IP3R isoforms.

  17. Investigation of the role of sigma1-receptors in inositol 1,4,5-trisphosphate dependent calcium signaling in hepatocytes.

    Science.gov (United States)

    Abou-Lovergne, A; Collado-Hilly, M; Monnet, F P; Koukoui, O; Prigent, S; Coquil, J F; Dupont, G; Combettes, L

    2011-07-01

    In hepatocytes, as in other cell types, Ca(2+) signaling is subject to complex regulations, which result largely from the intrinsic characteristics of the different inositol 1,4,5-trisphosphate receptor (InsP(3)R) isoforms and from their interactions with other proteins. Although sigma1 receptors (Sig-1Rs) are widely expressed in the liver, their involvement in hepatic Ca(2+) signaling remains unknown. We here report that in this cell type Sig-1R interact with type 1 isoforms of the InsP(3) receptors (InsP(3)R-1). These results obtained by immunoprecipitation experiments are confirmed by the observation that Sig-1R proteins and InsP(3)R-1 colocalize in hepatocytes. However, Sig-1R ligands have no effect on InsP(3)-induced Ca(2+) release in hepatocytes. This can be explained by the rather low expression level expression of InsP(3)R-1. In contrast, we find that Sig-1R ligands can inhibit agonist-induced Ca(2+) signaling via an inhibitory effect on InsP(3) synthesis. We show that this inhibition is due to the stimulation of PKC activity by Sig-1R, resulting in the well-known down-regulation of the signaling pathway responsible for the transduction of the extracellular stimulus into InsP(3) synthesis. The PKC sensitive to Sig-1R activity belongs to the family of conventional PKC, but the precise molecular mechanism of this regulation remains to be elucidated.

  18. Inositol 1,4,5-triphosphate drives glutamatergic and cholinergic inhibition selectively in spiny projection neurons in the striatum.

    Science.gov (United States)

    Clements, Michael A; Swapna, Immani; Morikawa, Hitoshi

    2013-02-06

    The striatum is critically involved in the selection of appropriate actions in a constantly changing environment. The spiking activity of striatal spiny projection neurons (SPNs), driven by extrinsic glutamatergic inputs, is shaped by local GABAergic and cholinergic networks. For example, it is well established that different types of GABAergic interneurons, activated by extrinsic glutamatergic and local cholinergic inputs, mediate powerful feedforward inhibition of SPN activity. In this study, using mouse striatal slices, we show that glutamatergic and cholinergic inputs exert direct inhibitory regulation of SPN activity via activation of metabotropic glutamate receptors (mGluRs) and muscarinic acetylcholine receptors. While pressure ejection of the group I mGluR (mGluR1/5) agonist DHPG [(S)-3,5-dihydroxyphenylglycine] equally engages both mGluR1 and mGluR5 subtypes, the mGluR-dependent component of IPSCs elicited by intrastriatal electrical stimulation is almost exclusively mediated by the mGluR1 subtype. Ca(2+) release from intracellular stores specifically through inositol 1,4,5-triphospahte receptors (IP(3)Rs) and not ryanodine receptors (RyRs) mediates this form of inhibition by gating two types of Ca(2+)-activated K(+) channels (i.e., small-conductance SK channels and large-conductance BK channels). Conversely, spike-evoked Ca(2+) influx triggers Ca(2+) release solely through RyRs to generate SK-dependent slow afterhyperpolarizations, demonstrating functional segregation of IP(3)Rs and RyRs. Finally, IP(3)-induced Ca(2+) release is uniquely observed in SPNs and not in different types of interneurons in the striatum. These results demonstrate that IP(3)-mediated activation of SK and BK channels provides a robust mechanism for glutamatergic and cholinergic inputs to selectively suppress striatal output neuron activity.

  19. Temperature-dependence and conformational basis of inositol 1,4,5-trisphosphate receptor regulated by Ca2+

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The inositol 1,4,5-trisphosphate (InsP3) receptor was purified from bovine cerebellum and reconstituted in liposomes composed of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) (1:1) successfully.No effect of Ca2+ concentration on [3H]-InsP3 binding to unreconstituted InsP3 receptor could be observed either at 4℃ or at 25℃,whereas the effect of [Ca2+] on reconstituted InsP3 receptor depended on the temperature.The Ca2+ concentration outside the proteolipsome ([Ca2+]o) had no detectable effect on InsP3 binding to InsP3 receptor at 4℃.In contrast,with increase of [Ca2+]o from 0 to 100 nmol/L at 25℃,the InsP3 binding activity increased gradually.Then the InsP3 binding activity was decreased drastically at higher [Ca2+]o and inhibited entirely at 50 mol/L [Ca2+]o.Conformational studies on intrinsic fluorescence of the reconstituted InsP3 receptor and its quenching by KI and HB indicated that the global conformation of reconstituted InsP3 receptor could not be affected by [Ca2+]o at 4℃.While at 25℃,the effects of 10 m mol/L [Ca2+]o on global,membrane and cytoplasmic conformation of the reconstituted InsP3 receptor were different significantly from that of 100 nmol/L [Ca2+]o.

  20. 腹腔镜下完全腹膜外疝修补术145例临床分析

    Institute of Scientific and Technical Information of China (English)

    刘祖军; 卢榜裕; 蔡小勇; 陆文奇; 江文枢

    2005-01-01

    目的:总结145例腹腔镜下完全腹膜外疝修补术的临床疗效。方法:回顾性分析该科2002年10月至2005年3月145例腹腔镜下完全腹膜外疝修补术的临床资料,均为男性,年龄18-91岁,平均52岁:单侧疝123例,双侧疝22例。疝类型:斜疝112例,直疝33例,

  1. Search for a standard-model-like Higgs boson with a mass in the range 145 to 1000 GeV at the LHC

    CERN Document Server

    Chatrchyan, Serguei; Sirunyan, Albert M; Tumasyan, Armen; Adam, Wolfgang; Bergauer, Thomas; Dragicevic, Marko; Erö, Janos; Fabjan, Christian; Friedl, Markus; Fruehwirth, Rudolf; Ghete, Vasile Mihai; Hörmann, Natascha; Hrubec, Josef; Jeitler, Manfred; Kiesenhofer, Wolfgang; Knünz, Valentin; Krammer, Manfred; Krätschmer, Ilse; Liko, Dietrich; Mikulec, Ivan; Rabady, Dinyar; Rahbaran, Babak; Rohringer, Christine; Rohringer, Herbert; Schöfbeck, Robert; Strauss, Josef; Taurok, Anton; Treberer-Treberspurg, Wolfgang; Waltenberger, Wolfgang; Wulz, Claudia-Elisabeth; Mossolov, Vladimir; Shumeiko, Nikolai; Suarez Gonzalez, Juan; Alderweireldt, Sara; Bansal, Monika; Bansal, Sunil; Cornelis, Tom; De Wolf, Eddi A; Janssen, Xavier; Knutsson, Albert; Luyckx, Sten; Mucibello, Luca; Ochesanu, Silvia; Roland, Benoit; Rougny, Romain; Van Haevermaet, Hans; Van Mechelen, Pierre; Van Remortel, Nick; Van Spilbeeck, Alex; Blekman, Freya; Blyweert, Stijn; D'Hondt, Jorgen; Kalogeropoulos, Alexis; Keaveney, James; Maes, Michael; Olbrechts, Annik; Tavernier, Stefaan; Van Doninck, Walter; Van Mulders, Petra; Van Onsem, Gerrit Patrick; Villella, Ilaria; Clerbaux, Barbara; De Lentdecker, Gilles; Gay, Arnaud; Hreus, Tomas; Léonard, Alexandre; Marage, Pierre Edouard; Mohammadi, Abdollah; Reis, Thomas; Thomas, Laurent; Vander Velde, Catherine; Vanlaer, Pascal; Wang, Jian; Adler, Volker; Beernaert, Kelly; Benucci, Leonardo; Cimmino, Anna; Costantini, Silvia; Dildick, Sven; Garcia, Guillaume; Klein, Benjamin; Lellouch, Jérémie; Marinov, Andrey; Mccartin, Joseph; Ocampo Rios, Alberto Andres; Ryckbosch, Dirk; Sigamani, Michael; Strobbe, Nadja; Thyssen, Filip; Tytgat, Michael; Walsh, Sinead; Yazgan, Efe; Zaganidis, Nicolas; Basegmez, Suzan; Bruno, Giacomo; Castello, Roberto; Ceard, Ludivine; Delaere, Christophe; Du Pree, Tristan; Favart, Denis; Forthomme, Laurent; Giammanco, Andrea; Hollar, Jonathan; Lemaitre, Vincent; Liao, Junhui; Militaru, Otilia; Nuttens, Claude; Pagano, Davide; Pin, Arnaud; Piotrzkowski, Krzysztof; Popov, Andrey; Selvaggi, Michele; Vizan Garcia, Jesus Manuel; Beliy, Nikita; Caebergs, Thierry; Daubie, Evelyne; Hammad, Gregory Habib; Alves, Gilvan; Correa Martins Junior, Marcos; Martins, Thiago; Pol, Maria Elena; Henrique Gomes E Souza, Moacyr; Aldá Júnior, Walter Luiz; Carvalho, Wagner; Chinellato, Jose; Custódio, Analu; Da Costa, Eliza Melo; De Jesus Damiao, Dilson; De Oliveira Martins, Carley; Fonseca De Souza, Sandro; Malbouisson, Helena; Malek, Magdalena; Matos Figueiredo, Diego; Mundim, Luiz; Nogima, Helio; Prado Da Silva, Wanda Lucia; Santoro, Alberto; Soares Jorge, Luana; Sznajder, Andre; Tonelli Manganote, Edmilson José; Vilela Pereira, Antonio; Souza Dos Anjos, Tiago; Bernardes, Cesar Augusto; De Almeida Dias, Flavia; Tomei, Thiago; De Moraes Gregores, Eduardo; Lagana, Caio; Da Cunha Marinho, Franciole; Mercadante, Pedro G; Novaes, Sergio F; Padula, Sandra; Genchev, Vladimir; Iaydjiev, Plamen; Piperov, Stefan; Rodozov, Mircho; Stoykova, Stefka; Sultanov, Georgi; Tcholakov, Vanio; Trayanov, Rumen; Vutova, Mariana; Dimitrov, Anton; Hadjiiska, Roumyana; Kozhuharov, Venelin; Litov, Leander; Pavlov, Borislav; Petkov, Peicho; Bian, Jian-Guo; Chen, Guo-Ming; Chen, He-Sheng; Jiang, Chun-Hua; Liang, Dong; Liang, Song; Meng, Xiangwei; Tao, Junquan; Wang, Jian; Wang, Xianyou; Wang, Zheng; Xiao, Hong; Xu, Ming; Asawatangtrakuldee, Chayanit; Ban, Yong; Guo, Yifei; Li, Qiang; Li, Wenbo; Liu, Shuai; Mao, Yajun; Qian, Si-Jin; Wang, Dayong; Zhang, Linlin; Zou, Wei; Avila, Carlos; Carrillo Montoya, Camilo Andres; Gomez, Juan Pablo; Gomez Moreno, Bernardo; Sanabria, Juan Carlos; Godinovic, Nikola; Lelas, Damir; Plestina, Roko; Polic, Dunja; Puljak, Ivica; Antunovic, Zeljko; Kovac, Marko; Brigljevic, Vuko; Duric, Senka; Kadija, Kreso; Luetic, Jelena; Mekterovic, Darko; Morovic, Srecko; Tikvica, Lucija; Attikis, Alexandros; Mavromanolakis, Georgios; Mousa, Jehad; Nicolaou, Charalambos; Ptochos, Fotios; Razis, Panos A; Finger, Miroslav; Finger Jr, Michael; Assran, Yasser; Ellithi Kamel, Ali; Mahmoud, Mohammed; Mahrous, Ayman; Radi, Amr; Kadastik, Mario; Müntel, Mait; Murumaa, Marion; Raidal, Martti; Rebane, Liis; Tiko, Andres; Eerola, Paula; Fedi, Giacomo; Voutilainen, Mikko; Härkönen, Jaakko; Karimäki, Veikko; Kinnunen, Ritva; Kortelainen, Matti J; Lampén, Tapio; Lassila-Perini, Kati; Lehti, Sami; Lindén, Tomas; Luukka, Panja-Riina; Mäenpää, Teppo; Peltola, Timo; Tuominen, Eija; Tuominiemi, Jorma; Tuovinen, Esa; Wendland, Lauri; Korpela, Arja; Tuuva, Tuure; Besancon, Marc; Choudhury, Somnath; Couderc, Fabrice

    2013-01-01

    A search for a standard-model-like Higgs boson in the H $\\to$ WW and H $\\to$ ZZ decay channels is reported, for Higgs boson masses in the range 145 < $m_H$ < 1000 GeV. The search is based upon proton-proton collision data samples corresponding to an integrated luminosity of up to 5.1 inverse femtobarns at $\\sqrt{s}$ = 7 TeV and up to 5.3 inverse femtobarns at $\\sqrt{s}$ = 8 TeV, recorded by the CMS experiment at the LHC. The combined upper limits at 95% confidence level on products of the cross section and branching fractions exclude a standard-model-like Higgs boson in the range 145 < $m_H$ < 710 GeV, thus extending the mass region excluded by CMS from 127-600 GeV up to 710 GeV.

  2. Photofission cross section and fissility of pre-actinide and intermediate-mass nuclei by 120- and 145-MeV Compton backscattered photons

    Energy Technology Data Exchange (ETDEWEB)

    Terranova, M.L. [Istituto Nazionale di Fisica Nucleare, Rome (Italy); Kezerashvili, G. Ya.; Milov, A.M. [Russian Academy of Sciences, Moscow (Russian Federation). Budker Inst. of Nuclear Physics] [and others

    1997-09-01

    Cross section measurements for photofission induced in {sup 209} Bi, {sup natP}b, {sup 197} Au, {sup nat} Pt, nat{sub w}, {sup 181} Ta, {sup 51} V and {sup nat} Ti by 120- and 145-MeV quasi-monochromatic photon beams have been performed at the ROKK-1M facility (BINP, Novosibirsk). The fission yields have been obtained using Makrofol sheets as solid-state fission track detectors. Nuclear fissility values have been deduced on the basis of Levinger`s modified quasi-deuteron model of photonuclear interaction, and compared with available literature data. The trend of fissility in the 60-145 MeV energy range has been analysed for various target nuclei as function of energy and of parameter Z{sup 2}/A. (author) 30 refs., 6 figs., 4 tabs.

  3. Photocatalytic removal of reactive yellow 145 dye from simulated textile wastewaters over supported (Co, Ni3O4/Al2O3 co-catalyst

    Directory of Open Access Journals (Sweden)

    Mohammad Emman J.

    2016-09-01

    Full Text Available The supported co-catalyst (Co, Ni3O4/Al2O3 was prepared via using a co-precipitation method. Three sets of these materials were prepared by calcination at three different temperatures 500, 600, and 700°C. Crystal structure of the prepared materials was investigated using powder X-rays diffraction (PXRD, Fourier transform infrared spectroscopy (FTIR, Atomic force microscope (AFM, and specific surface area (BET. The activity of the prepared catalysts was investigated by following both of photocatalytic and adsorption removal of Reactive yellow 145 dye (RY 145 from simulated industrial wastewaters. In this study, different reaction conditions were performed such as effect of pH of the reaction mixture, mass dosage of the used catalyst, and effect of temperature. In addition to that adsorption isotherms and reaction kinetics were investigated. Also the activity of these catalysts were investigated after cyclization of the used catalysts.

  4. In vitro proteolytic processing of the MD145 norovirus ORF1 nonstructural polyprotein yields stable precursors and products similar to those detected in calicivirus-infected cells.

    Science.gov (United States)

    Belliot, Gaël; Sosnovtsev, Stanislav V; Mitra, Tanaji; Hammer, Carl; Garfield, Mark; Green, Kim Y

    2003-10-01

    The MD145-12 strain (GII/4) is a member of the genus Norovirus in the Caliciviridae and was detected in a patient with acute gastroenteritis in a Maryland nursing home. The open reading frame 1 (ORF1) (encoding the nonstructural polyprotein) was cloned as a consensus sequence into various expression vectors, and a proteolytic cleavage map was determined. The virus-encoded cysteine proteinase mediated at least five cleavages (Q(330)/G(331), Q(696)/G(697), E(875)/G(876), E(1008)/A(1009), and E(1189)/G(1190)) in the ORF1 polyprotein in the following order: N-terminal protein; nucleoside triphosphatase; 20-kDa protein (p20); virus protein, genome linked (VPg); proteinase (Pro); polymerase (Pol). A time course analysis of proteolytic processing of the MD145-12 ORF1 polyprotein in an in vitro coupled transcription and translation assay allowed the identification of stable precursors and final mapped cleavage products. Stable precursors included p20VPg (analogous to the 3AB of the picornaviruses) and ProPol (analogous to the 3CD of the picornaviruses). Less stable processing intermediates were identified as p20VPgProPol, p20VPgPro, and VPgPro. The MD145-12 Pro and ProPol proteins were expressed in bacteria as active forms of the proteinase and used to further characterize their substrate specificities in trans cleavage assays. The MD145-12 Pro was able to cleave its five mapped cleavage sites in trans and, in addition, could mediate trans cleavage of the Norwalk virus (GI/I) ORF1 polyprotein into a similar proteolytic processing profile. Taken together, our data establish a model for proteolytic processing in the noroviruses that is consistent with nonstructural precursors and products identified in studies of caliciviruses that replicate in cell culture systems.

  5. Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells

    Directory of Open Access Journals (Sweden)

    Vucic V.

    2006-01-01

    Full Text Available Gamma-irradiation (gamma-IR is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of 60Co gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD, specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a significant (P < 0.001 antiproliferative and cytotoxic effect on DU 145 cells. Both effects were time and dose dependent. Also, the dose of gamma-IR which inhibited DNA synthesis and cell proliferation by 50% was 9.7 Gy. Furthermore, gamma-IR induced cell cycle arrest in the G2/M phase and the percentage of cells in the G2/M phase was increased from 15% (control to 49% (IR cells, with a nonsignificant induction of apoptosis. Treatment with 10 Gy gamma-IR for 24, 48, and 72 h stimulated CuZnSOD and MnSOD protein expression in a time-dependent manner, approximately by 3- to 3.5-fold. These data suggest that CuZnSOD and MnSOD enzymes may play an important role in the gamma-IR-induced changes in DU 145 cell growth, cell cycle arrest and cell death.

  6. Pharmacoproteomic study of the natural product Ebenfuran III in DU-145 prostate cancer cells: the quantitative and temporal interrogation of chemically induced cell death at the protein level.

    Science.gov (United States)

    Roumeliotis, Theodoros I; Halabalaki, Maria; Alexi, Xanthippi; Ankrett, Dyan; Giannopoulou, Eugenia G; Skaltsounis, Alexios-Leandros; Sayan, Berna S; Alexis, Michael N; Townsend, Paul A; Garbis, Spiros D

    2013-04-05

    A naturally occurring benzofuran derivative, Ebenfuran III (Eb III), was investigated for its antiproliferative effects using the DU-145 prostate cell line. Eb III was isolated from Onobrychis ebenoides of the Leguminosae family, a plant endemic in Central and Southern Greece. We have previously reported that Eb III exerts significant cytotoxic effects on certain cancer cell lines. This effect is thought to occur via the isoprenyl moiety at the C-5 position of the molecule. The study aim was to gain a deeper understanding of the pharmacological effect of Eb III on DU-145 cell death at the translational level using a relative quantitative and temporal proteomics approach. Proteins extracted from the cell pellets were subjected to solution phase trypsin proteolysis followed by iTRAQ-labeling. The labeled tryptic peptide extracts were then fractionated using strong cation exchange chromatography and the fractions were analyzed by nanoflow reverse phase ultraperformance liquid chromatography-nanoelectrospray ionization-tandem mass spectrometry analysis using a hybrid QqTOF platform. Using this approach, we compared the expression levels of 1360 proteins analyzed at ≤ 1% global protein false discovery rate (FDR), commonly present in untreated (control, vehicle only) and Eb III-treated cells at the different exposure time points. Through the iterative use of Ingenuity Pathway Analysis with hierarchical clustering of protein expression patterns, followed by bibliographic research, the temporal regulation of the Calpain-1, ERK2, PAR-4, RAB-7, and Bap31 proteins were identified as potential nodes of multipathway convergence to Eb III induced DU-145 cell death. These proteins were further verified with Western blot analysis. This gel-free, quantitative 2DLC-MS/MS proteomics method effectively captured novel modulated proteins in the DU-145 cell line as a response to Eb III treatment. This approach also provided greater insight to the multifocal and combinatorial signaling

  7. Control of ALS resistant volunteer oil seed rape and other dicotyledonous weeds with GF-145, a new cereal herbicide product containing isoxaben and florasulam

    Directory of Open Access Journals (Sweden)

    Becker, Jörg

    2014-02-01

    Full Text Available GF-145 contains the active ingredients isoxaben (610 g ai/kg and florasulam (40 g ai/kg and is formulated as a Wettable Granule (WG. The active ingredients are found in commercial products such as Primus™2 (florasulam, Starane XL™ (fluroxypyr + florasulam, Ariane C™ (fluroxypyr + florasulam + clopyralid or Flexidor™ (isoxaben. While florasulam has been widely used in cereal crops in recent years, isoxaben offers a new mode of action (MOA for use in German cereal herbicides even when considering that Flexidor™ has had regulatory approval in 1988 to 1991. The MOA of isoxaben is inhibition of cellulose synthesis (HRAC class L, while florasulam inhibits Acetolactate Synthase (ALS and is a representative of the HRAC class B. It is known that florasulam works through uptake by green leaves. Isoxaben is a herbicide with soil activity and with a very low activity when foliar applied, except on some species in the cruciferae family. GF-145 is intended to be applied in the autumn in cereals (wheat, barley, rye, triticale for the control of ALS resistant volunteer oil seed rape and annual dicotyledonous weeds including Matricaria spp., Stellaria media, Papaver rhoeas, Capsella bursa-pastoris, Myosotis arvensis, Lamium spp., Galium aparine, Veronica spp. and others when applied at early post-emergence from BBCH 10 to 13 of the crop. The use rate in winter cereals is 95 g product/ha (58 g ai/ha isoxaben plus 3.75 g ai/ha florasulam. Field trials conducted in previous years confirmed excellent selectivity in all cereal crops and efficacy trials initiated in autumn 2012 show that GF-145 provides excellent and superior control to ALS resistant oil seed rape that was better than straight florasulam and other ALS active ingredients. GF-145 adds a new MOA to the cereal herbicide portfolio and controls volunteer oil seed rape, cruciferous weeds and broad-leaved weeds and is more robust than florasulam based products that do not contain isoxaben.

  8. Oral vitamins C and E as additional treatment in patients with acute anterior uveitis: a randomised double masked study in 145 patients

    OpenAIRE

    van Rooij, Jeroen; Schwartzenberg, S.G.; Baarsma, Seerp; Mulder, Paul

    1999-01-01

    textabstractAIM: To investigate the effect of additional oral vitamins C and E on acute anterior uveitis. METHODS: A placebo controlled double masked study on the effect of vitamin C 500 mg in combination with vitamin E 100 mg twice daily in 145 patients with acute anterior uveitis. As a primary end point variable, laser cell/flare measurements were performed. Best corrected and stenopeic visual acuity (VA) testing and clinical variable scores were measured. RESULTS: Laser flare measurements ...

  9. The effect of tyrphostins AG494 and AG1478 on the autocrine growth regulation of A549 and DU145 cells

    Directory of Open Access Journals (Sweden)

    Agnieszka Bojko

    2012-07-01

    Full Text Available We employed two selective EGFR tyrosine kinase inhibitors: AG494 (reversible and AG1478 (irreversible for growth regulation of human lung (A549 and prostate (DU145 cancer cell lines, cultured in chemically defined DMEM/F12 medium. Both tested tyrphostins significantly inhibited autocrine growth of the investigated cell lines. The action of AG494 was dose dependent, and at highest concentrations led to complete inhibition of growth. AG1478 seemed to be more effective at lower concentrations, but was unable to completely inhibit growth of A549 cells. Inhibition of EGFR kinase activity by AG494 in contrast to AG1478 had no effect on the activity of ERK in both cell lines. Both EGFR’s inhibitors induced apoptosis of the investigated lung and prostate cancer cell lines, but the proapoptotic effect of the investigated tyrphostins was greater in A549 than in DU145 cells. The tyrphostins arrested cell growth of DU145 and A549 cells in the G1 phase, similarly to other known inhibitors of EGFR. The influence of AG494 and AG1478 on the activity of two signaling proteins (AKT and ERK was dependent upon the kind of investigated cells. In the case of DU145 cells, there was an evident decline in enzymatic activity of both kinases (stronger for AG1478, while in A549, only AG1478 effectively inhibited the phosphorylation of Akt. Tyrphostins AG494 and AG1478 are ATP-competitors and are supposed to have a similar mechanism of action, but our results suggest that this is not quite true.

  10. Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells

    Directory of Open Access Journals (Sweden)

    V. Vucic

    2006-02-01

    Full Text Available Gamma-irradiation (gamma-IR is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of 60Co gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD, specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a significant (P < 0.001 antiproliferative and cytotoxic effect on DU 145 cells. Both effects were time and dose dependent. Also, the dose of gamma-IR which inhibited DNA synthesis and cell proliferation by 50% was 9.7 Gy. Furthermore, gamma-IR induced cell cycle arrest in the G2/M phase and the percentage of cells in the G2/M phase was increased from 15% (control to 49% (IR cells, with a nonsignificant induction of apoptosis. Treatment with 10 Gy gamma-IR for 24, 48, and 72 h stimulated CuZnSOD and MnSOD protein expression in a time-dependent manner, approximately by 3- to 3.5-fold. These data suggest that CuZnSOD and MnSOD enzymes may play an important role in the gamma-IR-induced changes in DU 145 cell growth, cell cycle arrest and cell death.

  11. Association between functional polymorphisms in the promoter of the miR-143/145 cluster and risk of intracranial aneurysm

    Science.gov (United States)

    Sima, Xiutian; Sun, Hong; Zhou, Peizhi; You, Chao; Cai, Bowen

    2017-01-01

    MicroRNAs (miRs)-143/145 are involved in various biological processes related to aneurysm formation and are downregulated in patients with intracranial aneurysm (IA). We aimed to determine whether two functional polymorphisms (i.e. rs4705342 and rs4705343) in the promoter of miR-143/145 are related to IA risk. A case-control study was undertaken to examine the association of rs4705342 and rs4705343 with IA risk, including 565 patients with IA and 622 age- and gender-matched controls. rs4705342 was analysed by TaqMan Assay, and rs4705343 was genotyped using polymerase chain reaction-restriction fragment length polymorphism. miR-143/145 expression was quantified using RT-PCR. rs4705342 was associated with a significantly lower risk of IA, with adjusted ORs of 0.74 (95% CI: 0.58–0.95) for TC genotype carriers and 0.74 (95% CI: 0.59–0.94) for TC/CC genotypes carriers. Individuals carrying the rs4705342 C allele had a reduced risk of IA (adjusted OR = 0.82; 95% CI: 0.68–0.98). Haplotype of the two loci of rs4705342 and rs4705343 showed that the CT haplotype carried a lower IA risk and higher miR-143 level. Moreover, the rs4705342 CC/CT genotypes were associated with higher miR-143 levels. Thus, the rs4705342C-rs4705343T haplotype in the promoter of miR-143/145 cluster may be related to IA development. PMID:28272526

  12. Optical wireless transmission of 405 nm, 1.45 Gbit/s optical IM/DD-OFDM signals through a 4.8 m underwater channel.

    Science.gov (United States)

    Nakamura, Kazuhiko; Mizukoshi, Izumi; Hanawa, Masanori

    2015-01-26

    In this paper, we experimentally demonstrate wireless transmission of optical intensity modulation/direct detection-orthogonal frequency division multiplexing (IM/DD-OFDM) signals in an underwater channel using a field programmable gate array based real-time transmitter. The real-time transmission of a 405 nm 1.45 Gbit/s optical OFDM signal through a 4.8 m underwater channel with an error vector magnitude of approximately 10% was successfully achieved.

  13. The E1 copper binding domain of full-length amyloid precursor protein mitigates copper-induced growth inhibition in brain metastatic prostate cancer DU145 cells

    Energy Technology Data Exchange (ETDEWEB)

    Gough, Mallory, E-mail: m.gough1@lancaster.ac.uk; Blanthorn-Hazell, Sophee, E-mail: s.blanthorn-hazell@lancaster.ac.uk; Delury, Craig, E-mail: c.delury@lancaster.ac.uk; Parkin, Edward, E-mail: e.parkin@lancaster.ac.uk

    2014-10-31

    Highlights: • Copper levels are elevated in the tumour microenvironment. • APP mitigates copper-induced growth inhibition of DU145 prostate cancer (PCa) cells. • The APP intracellular domain is a prerequisite; soluble forms have no effect. • The E1 CuBD of APP is also a prerequisite. • APP copper binding potentially mitigates copper-induced PCa cell growth inhibition. - Abstract: Copper plays an important role in the aetiology and growth of tumours and levels of the metal are increased in the serum and tumour tissue of patients affected by a range of cancers including prostate cancer (PCa). The molecular mechanisms that enable cancer cells to proliferate in the presence of elevated copper levels are, therefore, of key importance in our understanding of tumour growth progression. In the current study, we have examined the role played by the amyloid precursor protein (APP) in mitigating copper-induced growth inhibition of the PCa cell line, DU145. A range of APP molecular constructs were stably over-expressed in DU145 cells and their effects on cell proliferation in the presence of copper were monitored. Our results show that endogenous APP expression was induced by sub-toxic copper concentrations in DU145 cells and over-expression of the wild-type protein was able to mitigate copper-induced growth inhibition via a mechanism involving the cytosolic and E1 copper binding domains of the full-length protein. APP likely represents one of a range of copper binding proteins that PCa cells employ in order to ensure efficient proliferation despite elevated concentrations of the metal within the tumour microenvironment. Targeting the expression of such proteins may contribute to therapeutic strategies for the treatment of cancers.

  14. DNA methyltrans-ferase 3b regulating the Cyclin D1 gene expression by microRNA-145 in the human hepatocellular carcinoma cell line%肝癌细胞DNA甲基转移酶3b通过微小RNA-145调节细胞周期素D1基因表达的研究

    Institute of Scientific and Technical Information of China (English)

    王佳辰; 司亚卿; 秦贯军

    2014-01-01

    Objective To discuss the mechanism of the DNA methyltrans-ferase 3b (DNMT3b) regulating the Cyclin D1 gene expression in SMMC7721 cell line.Methods DNMT3b small interfering RNA (siRNA) was transfected into SMMC-7721 cells.Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of the microRNA (miR)-145 and Cyclin D1 mRNA.MSP was used to detect whether the promoter of miR-145 was methylated.Results DNMT3b has been successfully suppressed in the culture cells transfected by miR-145 with a transfection efficiency over 90% ; The methylation status of miR-145 gene showed no significant differences between two groups (P >0.05) ; The expression of miR-145 was significantly higher than in control group (P < 0.01) and the change between two groups had no difference (P > 0.05).Conclusion DNMT3b can regulate the expression of Cyclin D1 by miR-145,affect the cell cycle and cause a large number of apoptosis of SMMC-7721 cells.%目的 探讨DNA甲基转移酶3b(DNMT3b)在人肝癌细胞株(SMMC7721)中调节细胞周期素(Cyclin) D1基因表达的机制.方法 用DNMT3b的小干扰RNA(siRNA)表达载体转染SMMC7721细胞抑制DNMT3b的表达;采用逆转录-聚合酶链反应(RT-PCR)检测微小RNA(miR)-145、Cyclin D1 mRNA表达的变化;用甲基化特异性PCR (MSP)技术检测miR-145基因启动子区甲基化状态的变化.结果 siRNA转染肝癌细胞的效率可达90%以上,DNMT3b成功被抑制;两组中miR-145基因启动子区甲基化水平的差异无统计学意义(P>0.05);实验组miR-145表达水平明显高于对照组(P<0.01),但Cylin D1 mRNA表达水平的差异无统计学意义(P>0.05).结论 DNMT3b可以通过miR-145调节Cyclin D1的表达,从而影响细胞周期,进而引起肝癌细胞的大量凋亡.

  15. Effects of Oridonin Induced Apoptosis in Human Androgen-independent Ptostate Cacer Cell Line DU145 by Inhibition of the AKT Signaling Pathway%冬凌草甲素通过抑制Akt通路诱导人雄激素非依赖性前列腺癌细胞DU145细胞凋亡

    Institute of Scientific and Technical Information of China (English)

    陈科; 程帆; 黄保成; 卢强

    2016-01-01

    目的:观察冬凌草甲素对人雄激素非依赖性前歹列腺癌细胞DU145细胞增殖、凋亡的影响,并分析可能的作用机制.方法:体外培养的DU145细胞,用不同浓度冬凌草甲素(浓度0、10、20、40、80、100μmol/L)处理24、48、72 h,MTT法测定其对DU145细胞增殖的影响;冬凌草甲素(浓度分别为0、20、40、80μmol/L)处理DU145细胞48 h后,采用流式细胞仪检测细胞凋亡,Westem blot法检测Akt、p-Akt、p-GSK-3β、Bax及Bcl-2蛋白的表达情况.结果:冬凌草甲素对DU145细胞的增殖具有抑制作用,且呈剂量、时间依赖性;DU145细胞经冬凌草甲素作用48 h后,细胞凋亡率显著增加;Bax表达上调,而Bcl-2表达下调;p-Akt及其下游蛋白p-GSK-3β的表达降低.结论:冬凌草甲素可通过抑制DU145细胞增殖并促进其凋亡,其作用机制可能与抑制Akt信号通路激活,继而上调Bax并下调Bcl-2有关.%Objective:To explore the effect of oridonin on cell proliferation,apoptosis and telomerase activity in human androgen-independent ptostate cacer cell line DU14.Methods:DU145 cells were cultured in vitro.After treatment by oridonin at different concentrations respectively at different time,the cell viability was determined by the MTT method.Apoptosis was analyzed by flow cytometry.Western blot were used for Akt,p-Akt,p-GSK-3 β,Bax and Bcl-2 protein analysis.Results:From the data of MTT,the cell proliferation of DU145 cells was inhibited by oridonin in a dose-dependent and time-dependent manner.Flow cytometry assays demonstrated that oridonin could promote cell apoptosis.the apoptosis rate of DU145cells increased from 16.7% to 37.5% in oridonin group.The treatment with oridonin promoted the expression of pro-apoptotic factor Bax,and suppressed the expression of anti-apoptotic factor Bcl-2.Furthermore,the data of western blot showed that oridonin decreased the expression of p-AKT and downstream effector of Akt,p-GSK-3 β in a dose-dependent manner

  16. Combination of p53(ser15) and p21/p21(thr145) in peripheral blood lymphocytes as potential Alzheimer's disease biomarkers.

    Science.gov (United States)

    Tan, Mengshan; Wang, Shuying; Song, Juexian; Jia, Jianping

    2012-05-16

    Alzheimer's disease (AD) is still difficult to be precisely diagnosed in its early stage to date. Establishing of reliable and manageable disease-specific biological markers is required to improve diagnostic accuracy. Based on the hypothesis of cell cycle regulatory failure at the early stage of AD, we tested whether cell cycle regulating proteins p53, p21 and their phosphorylated forms p53(ser15), p21(thr145) were changed in AD patients and whether these proteins could be used as diagnostic biomarkers. Western bolt, Enzyme-linked immunosorbent assay (ELISA), immunofluorescent staining and flow cytometry (FCM) analysis were employed to analyze levels of these proteins in peripheral blood lymphocytes (PBLs) from 95 controls, 94 AD, 12 Parkinson's disease (PD) and 15 vascular dementia (VaD) patients. Compared with controls, p53(ser15) and p21(thr145) levels were significantly increased and p21 level was significantly decreased in PBLs of AD patients but not in PD or VaD, while p53 was increased in both AD and VaD patients. The receiver operating characteristic (ROC) curve analysis showed that the specificity and sensitivity were 76% and 84% for p53, 88% and 82% for p53(ser15), 80% and 75% for p21 and 84% and 68% for p21(thr145) in identifying AD patients. The relatively high diagnostic accuracy support these proteins, especially p53(ser15) and p21 in PBLs may become potential biomarkers for diagnosis of AD.

  17. Outbreak of verocytotoxin-producing E. coli O145 and O26 infections associated with the consumption of ice cream produced at a farm, Belgium, 2007.

    Science.gov (United States)

    De Schrijver, K; Buvens, G; Possé, B; Van den Branden, D; Oosterlynck, O; De Zutter, L; Eilers, K; Piérard, D; Dierick, K; Van Damme-Lombaerts, R; Lauwers, C; Jacobs, R

    2008-02-14

    In October 2007, an outbreak of verocytotoxin-producing Escherichia coli (VTEC) O145 and E. coli O26 occurred among consumers of ice cream produced and sold in September 2007 at a farm in the province of Antwerp (Belgium). The ice cream was consumed at two birthday parties and also eaten at the farm. Five children, aged between two and 11 years, developed haemolytic uraemic syndrome (HUS), and seven other co-exposed persons contracted severe diarrhoea. In three of the five HUS cases VTEC O145 infections were laboratory confirmed, one in association with VTEC O26. Identical isolates of E. coli O145 and O26 were detected with PCR and PFGE in faecal samples of patients and in ice cream leftovers from one of the birthday parties, in faecal samples taken from calves, and in samples of soiled straw from the farm at which the ice cream was produced. Ice cream was made from pasteurised milk and most likely contaminated by one of food handlers.

  18. Respiratory Syndrome Virus%Marc-145细胞培养条件摸索及其初步应用

    Institute of Scientific and Technical Information of China (English)

    兰海楠; 张辉; 崔焕忠; 杜东菊; 孔金超; 赵小丽; 郑鑫

    2011-01-01

    The Marc-145 cells,epithelioid cell,passage cell,originate from the monkey kidney cell,it is cloned from mother cell (Mar-104 cell). This cell is mainly used for virus's culture,especially the porcine reproductive and respiratory syndrome virus (PRRSV), this thesis mainly summarize the cultural method of this cell,and analyze reasons about the condition of virus in cells.%Marc-145细胞,上皮样细胞,来源于猴肾细胞,从母细胞(Ma-104细胞)克隆而得到,可连续传代培养.此细胞主要用于病毒的培养,特别是猪繁殖与呼吸综合征病毒(PRRSV)对此细胞尤为敏感,本试验主要研究Marc-145的适宜培养条件,以及PRRSV野毒株在该细胞中的增殖.

  19. Dairy Streptococcus thermophilus improves cell viability of Lactobacillus brevis NPS-QW-145 and its γ-aminobutyric acid biosynthesis ability in milk.

    Science.gov (United States)

    Wu, Qinglong; Law, Yee-Song; Shah, Nagendra P

    2015-08-06

    Most high γ-aminobutyric acid (GABA) producers are Lactobacillus brevis of plant origin, which may be not able to ferment milk well due to its poor proteolytic nature as evidenced by the absence of genes encoding extracellular proteinases in its genome. In the present study, two glutamic acid decarboxylase (GAD) genes, gadA and gadB, were found in high GABA-producing L. brevis NPS-QW-145. Co-culturing of this organism with conventional dairy starters was carried out to manufacture GABA-rich fermented milk. It was observed that all the selected strains of Streptococcus thermophilus, but not Lactobacillus delbrueckii subsp. bulgaricus, improved the viability of L. brevis NPS-QW-145 in milk. Only certain strains of S. thermophilus improved the gadA mRNA level in L. brevis NPS-QW-145, thus enhanced GABA biosynthesis by the latter. These results suggest that certain S. thermophilus strains are highly recommended to co-culture with high GABA producer for manufacturing GABA-rich fermented milk.

  20. Identified Light and Strange Hadron Spectra at √sNN = 14.5 GeV with STAR at RHIC BES I

    Science.gov (United States)

    Brandenburg, James Daniel

    2016-08-01

    With the recently measured Au+Au collisions at √sNN=14.5 GeV, RHIC completed its first phase of the Beam Energy Scan (BES) program. The main motivation of the BES program is the search for a conjectured critical point and possible first order phase transition. Amongst the various collision energies of 7.7, 11.5, 19.6, 27, and 39 GeV, that have been previously presented by STAR, collisions at 14.5 GeV will provide data set in the relatively large chemical potential gap between the 11.5 and 19.6 GeV center-of-mass energies. In this contribution, we report new STAR measurements of Au+Au at √sNN=14.5 GeV that include identified light particle RCP and spectra, as well as measurements of the strange hadrons (K0 s, A, ξ, and ω). The spectra from both light and strange particles cover a significant range of the intermediate transverse momentum (2 < pT < 5 GeV/c) in all beam energies. We will discuss the physics implications of these observables and whether hadronic or partonic interactions dominate the collision dynamics at a given center-of-mass energy.

  1. The E1 copper binding domain of full-length amyloid precursor protein mitigates copper-induced growth inhibition in brain metastatic prostate cancer DU145 cells.

    Science.gov (United States)

    Gough, Mallory; Blanthorn-Hazell, Sophee; Delury, Craig; Parkin, Edward

    2014-10-31

    Copper plays an important role in the aetiology and growth of tumours and levels of the metal are increased in the serum and tumour tissue of patients affected by a range of cancers including prostate cancer (PCa). The molecular mechanisms that enable cancer cells to proliferate in the presence of elevated copper levels are, therefore, of key importance in our understanding of tumour growth progression. In the current study, we have examined the role played by the amyloid precursor protein (APP) in mitigating copper-induced growth inhibition of the PCa cell line, DU145. A range of APP molecular constructs were stably over-expressed in DU145 cells and their effects on cell proliferation in the presence of copper were monitored. Our results show that endogenous APP expression was induced by sub-toxic copper concentrations in DU145 cells and over-expression of the wild-type protein was able to mitigate copper-induced growth inhibition via a mechanism involving the cytosolic and E1 copper binding domains of the full-length protein. APP likely represents one of a range of copper binding proteins that PCa cells employ in order to ensure efficient proliferation despite elevated concentrations of the metal within the tumour microenvironment. Targeting the expression of such proteins may contribute to therapeutic strategies for the treatment of cancers.

  2. Classificação dos linfomas não-Hodgkin: estudo morfológico e imunoistoquímico de 145 casos Classification of non-Hodgkin's lymphoma: morphological and immunological study of 145 cases

    Directory of Open Access Journals (Sweden)

    Cristiane Bedran Milito

    2002-01-01

    Full Text Available A classificação dos linfomas não-Hodgkin tem sido, ao longo dos últimos trinta anos, motivo de controvérsia. Várias classificações têm sido propostas em busca de um consenso entre patologistas e clínicos. Este trabalho teve como objetivo analisar criticamente três destas classificações através do estudo retrospectivo de 145 casos de linfomas primários de gânglio linfático selecionados do Serviço de Anatomia Patológica do Hospital Universitário Clementino Fraga Filho, entre 1979 e 1995. Os casos revistos foram classificados pelas propostas da Working Formulation, de Kiel e da Real. Testes imunoistoquímicos com os anticorpos anti-CD45, anti-CD20, anti-CD45RO e anti-CD30 foram realizados. Cento e sete casos (73,7% apresentaram fenótipo B; 33 casos (22,7%, fenótipo T; e quatro casos foram nulos (linfoma anaplásico de grandes células. Foi possível prever o fenótipo pela morfologia em 89,4% dos casos. Os linfomas de alto grau predominaram (59,2%, sendo o linfoma centroblástico o de maior freqüência (31,7% . Os linfomas foliculares representaram 29 casos (20%, com maior incidência dos de grandes células (31% do que dos de pequenas células (27,5%. Quando comparadas as três classificações, observamos que determinados grupos da Working Formulation abrigam múltiplas entidades. Isto se deve ao fato de a classificação da Working Formulation ser baseada somente em achados morfológicos e, por isso, deve ter seu uso desaconselhado. Já a classificação de Kiel e a da Real devem ter o seu emprego estimulado, pois apresentam, além de uma boa análise histopatológica, um estudo imunológico que define entidades biológicas correlacionando-se, quando possível, com a célula de origem.The non-Hodgkin's lymphomas classifications have been a controversial reason for the last thirty years. Many classifications have been proposed trying to achieve a consensus among pathologists and clinicians. The objective of this study was

  3. Pre- and post-processing of hydro-meteorological ensembles for the Norwegian flood forecasting system in 145 basins.

    Science.gov (United States)

    Jahr Hegdahl, Trine; Steinsland, Ingelin; Merete Tallaksen, Lena; Engeland, Kolbjørn

    2016-04-01

    Probabilistic flood forecasting has an added value for decision making. The Norwegian flood forecasting service is based on a flood forecasting model that run for 145 basins. Covering all of Norway the basins differ in both size and hydrological regime. Currently the flood forecasting is based on deterministic meteorological forecasts, and an auto-regressive procedure is used to achieve probabilistic forecasts. An alternative approach is to use meteorological and hydrological ensemble forecasts to quantify the uncertainty in forecasted streamflow. The hydrological ensembles are based on forcing a hydrological model with meteorological ensemble forecasts of precipitation and temperature. However, the ensembles of precipitation are often biased and the spread is too small, especially for the shortest lead times, i.e. they are not calibrated. These properties will, to some extent, propagate to hydrological ensembles, that most likely will be uncalibrated as well. Pre- and post-processing methods are commonly used to obtain calibrated meteorological and hydrological ensembles respectively. Quantitative studies showing the effect of the combined processing of the meteorological (pre-processing) and the hydrological (post-processing) ensembles are however few. The aim of this study is to evaluate the influence of pre- and post-processing on the skill of streamflow predictions, and we will especially investigate if the forecasting skill depends on lead-time, basin size and hydrological regime. This aim is achieved by applying the 51 medium-range ensemble forecast of precipitation and temperature provided by the European Center of Medium-Range Weather Forecast (ECMWF). These ensembles are used as input to the operational Norwegian flood forecasting model, both raw and pre-processed. Precipitation ensembles are calibrated using a zero-adjusted gamma distribution. Temperature ensembles are calibrated using a Gaussian distribution and altitude corrected by a constant gradient

  4. Type Ⅰ inositol 1,4,5-triphosphate receptors increase in kidney of mice with fulminant hepatic failure

    Institute of Scientific and Technical Information of China (English)

    Ying Wen; Wei Cui; Pei Liu

    2007-01-01

    AIM:To delineate the mechanisms of renal vasoconstriction in hepatorenal syndrome (HRS),we investigated the expression of type Ⅰ inositol 1,4,5-triphosphate receptors (IP3R Ⅰ) of kidney in mice with fulminant hepatic failure (FHF).METHODS:FHF was induced by lipopolysaccharide (LPS) in D-galactosamine (GaIN) sensitized BALB/c mice.There were 20 mice in normal saline (NS)-treated group,20 mice in LPS-treated group,20 mice in GaINtreated group,and 60 mice in GaIN/LPS-treated group (FHF group).Liver and kidney tissues were obtained at 2,6,and 9 h after administration.The liver and kidney specimens were stained with hematoxylin-eosin for studying morphological changes under light microscope.The expression of IP3R Ⅰ in kidney tissue was tested by immunohistochemistry,Western blot and reverse transcription (RT)-PCR.RESULTS:Kidney tissues were morphologically normal at all time points in all groups.IP3R Ⅰ proteins were found localized in the plasma region of glomerular mesangial cells (GMC) and vascular smooth muscle cells (VSMC) in kidney by immunohistochemical staining.In kidney of mice with FHF at 6 h and 9 h IP3R Ⅰ staining was upregulated.Results from Western blot demonstrated consistent and significant increment of IP3R Ⅰ expression in mice with FHF at 6 h and 9 h (t = 3.16,P<0.05;t = 5.43,P<0.01).Furthermore,we evaluated IP3R Ⅰ mRNA expression by RT-PCR and observed marked upregulation of IP3R Ⅰ mRNA in FHF samples at 2 h,6 h and 9 h compared to controls (t = 2.97,P<0.05;t = 4.42,P<0.01;t = 3.81,P<0.01).CONCLUSION:The expression of IP3R Ⅰ protein increased in GMC and renal VSMC of mice with FHF,possibly caused by up-regulation of IP3R Ⅰ mRNA.

  5. Glycoprotein 5 of porcine reproductive and respiratory syndrome virus strain SD16 inhibits viral replication and causes G2/M cell cycle arrest, but does not induce cellular apoptosis in Marc-145 cells

    Energy Technology Data Exchange (ETDEWEB)

    Mu, Yang, E-mail: muyang@nwsuaf.edu.cn [Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A& F University, No. 22 Xinong Road, Yangling, Shaanxi 712100 (China); Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People' s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100 (China); Li, Liangliang, E-mail: lifeiyang2007@126.com [Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A& F University, No. 22 Xinong Road, Yangling, Shaanxi 712100 (China); Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People' s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100 (China); Zhang, Beibei, E-mail: diana851218@163.com [Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A& F University, No. 22 Xinong Road, Yangling, Shaanxi 712100 (China); Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People' s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100 (China); Huang, Baicheng, E-mail: hbch228@163.com [Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A& F University, No. 22 Xinong Road, Yangling, Shaanxi 712100 (China); Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People' s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100 (China); Gao, Jiming, E-mail: jimingao2006@163.com [Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A& F University, No. 22 Xinong Road, Yangling, Shaanxi 712100 (China); Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture of the People' s Republic of China, No. 22 Xinong Road, Yangling, Shaanxi 712100 (China); and others

    2015-10-15

    Cell apoptosis is common after infection with porcine reproductive and respiratory syndrome virus (PRRSV). PRRSV GP5 has been reported to induce cell apoptosis. To further understand the role of GP5 in PRRSV induced cell apoptosis, we established Marc-145 cell lines stably expressing full-length GP5, GP5{sup Δ84-96} (aa 84-96 deletion), and GP5{sup Δ97-119} (aa 97-119 deletion). Cell proliferation, cell cycle progression, cell apoptosis and virus replication in these cell lines were evaluated. Neither truncated nor full-length GP5 induced cell apoptosis in Marc-145 cells. However, GP5{sup Δ97-119}, but not full-length or GP5{sup Δ84-96}, induced a cell cycle arrest at the G2/M phase resulting in a reduction in the growth of Marc-145 cells. Additionally, GP5{sup Δ84-96} inhibited the replication of PRRSV in Marc-145 cells through induction of IFN-β. These findings suggest that PRRSV GP5 is not responsible for inducing cell apoptosis in Marc-145 cells under these experimental conditions; however it has other important roles in virus/host cell biology. - Highlights: • Marc-145 cell lines stable expression PRRSV GP5 or truncated GP5 were constructed. • GP5{sup Δ97-119} expression in Marc-145 cell induced cell cycle arrest at G2/M phase. • Expression of GP5 and truncated GP5 could not induce Marc-145 cells apoptosis. • PRRSV replication in Marc-145-GP5{sup Δ84-96} was significantly inhibited.

  6. Plumbagin elicits differential proteomic responses mainly involving cell cycle, apoptosis, autophagy, and epithelial-to-mesenchymal transition pathways in human prostate cancer PC-3 and DU145 cells

    Directory of Open Access Journals (Sweden)

    Qui JX

    2015-01-01

    Full Text Available Jia-Xuan Qiu,1,2 Zhi-Wei Zhou, 3,4 Zhi-Xu He,4 Ruan Jin Zhao,5 Xueji Zhang,6 Lun Yang,7 Shu-Feng Zhou,3,4 Zong-Fu Mao11School of Public Health, Wuhan University, Wuhan, Hubei, People’s Republic of China; 2Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People’s Republic of China; 3Department of Pharmaceutical Sciences, College of Pharmacy, University of South Florida, Tampa, FL, USA; 4Guizhou Provincial Key Laboratory for Regenerative Medicine, Stem Cell and Tissue Engineering Research Center and Sino-US Joint Laboratory for Medical Sciences, Guiyang Medical University, Guiyang, Guizhou, People’s Republic of China; 5Center for Traditional Chinese Medicine, Sarasota, FL, USA; 6Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing, People’s Republic of China; 7Bio-X Institutes, Key Laboratory for the Genetics of Development and Neuropsychiatric Disorders (Ministry of Education, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaAbstract: Plumbagin (PLB has exhibited a potent anticancer effect in preclinical studies, but the molecular interactome remains elusive. This study aimed to compare the quantitative proteomic responses to PLB treatment in human prostate cancer PC-3 and DU145 cells using the approach of stable-isotope labeling by amino acids in cell culture (SILAC. The data were finally validated using Western blot assay. First, the bioinformatic analysis predicted that PLB could interact with 78 proteins that were involved in cell proliferation and apoptosis, immunity, and signal transduction. Our quantitative proteomic study using SILAC revealed that there were at least 1,225 and 267 proteins interacting with PLB and there were 341 and 107 signaling pathways and cellular functions potentially regulated by PLB in PC-3 and DU145 cells, respectively. These proteins and pathways played a

  7. 蝎毒多肽提取物诱导前列腺癌DU-145细胞凋亡的实验研究%Polypeptide Extract from Scorpion Venom (PESV)Induces Apoptosis of DU-145 Cells in Vitro

    Institute of Scientific and Technical Information of China (English)

    张月英; 张维东; 贾青; 王兆朋; 黄山英; 宋守芹; 王朝霞

    2006-01-01

    目的探讨蝎毒多肽提取物(PESV)诱导前列腺癌DU-145细胞凋亡的作用及机制.方法用PESV(40 μg/mL)处理DU-145细胞,采用Gimesa染色法观察凋亡细胞形态变化;采用免疫组化S-P法检测核增殖抗原Ki-67及凋亡相关基因bax和bcl-2的表达,并用病理图像分析软件进行半定量分析;TUNEL法检测凋亡细胞,并计算前列腺癌细胞增殖指数(proliferating index,PI)和凋亡指数(apoptosis index,AI).结果 PESV在体外对DU-145细胞有中度增殖抑制效应;在PESV作用下,DU-145细胞出现显著的细胞凋亡征象,凋亡指数明显增高,增殖指数降低,AI/PI明显增高(P<0.05).PESV处理DU-145细胞可明显提高凋亡相关基因bax表达水平,降低凋亡抑制蛋白Bcl-2表达水平,使Bcl-2/Bax比值明显减小(P<0.05).结论 PESV(40 μg/mL)可以诱导细胞凋亡,而且至少是通过促进Bax、抑制Bcl-2基因表达的机制诱导细胞凋亡.

  8. Anacardic acid induces apoptosis of prostate cancer Du145 cells through inhibiting expression of COX2, C-myc and VEGFA%槚如酸对雄激素非依赖型前列腺癌细胞株Du145的作用和对 COX2、C-myc、VEGFA 表达的影响及意义

    Institute of Scientific and Technical Information of China (English)

    谭靖; 蒋先镇; 汤育新; 龙智; 蒋志强; 戴英波; 何乐业

    2012-01-01

      目的探讨药物槚如酸对雄激素非依赖型前列腺癌细胞株 Du145的抗肿瘤作用及对癌基因 COX2、C-myc、VEGFA 表达的影响及意义。方法培养 Du145细胞株,使用 MTT 法测定不同浓度槚如酸对 Du145细胞增殖的影响,获得药物最佳作用时间和剂量;使用流式细胞仪检测槚如酸对 Du145细胞周期的影响和对细胞凋亡的作用;并通过 RT-PCR 及 Western blot 来测定槚如酸对 Du145细胞内癌基因 C-myc、COX2、VEGFA的 mRNA 和蛋白表达的影响。结果槚如酸抑制 Du145细胞增殖,其作用具有剂量、时间效应关系;槚如酸诱导 Du145细胞凋亡,并阻滞细胞周期于 G1/S 期;槚如酸处理后,Du145细胞株内癌基因 COX2、C-myc、VEGFA 的 mRNA 和蛋白表达均降低。结论槚如酸对雄激素非依赖型前列腺癌 Du145细胞具致死作用,其抗癌机制值得进一步探讨。%10.3969/j.issn.1008-0848.2012.12.002

  9. Prevalence and pathogen load of Campylobacter spp., Salmonella enterica and Escherichia coli O157/O145 serogroup in sheep faeces collected at sale yards and in abattoir effluent in Western Australia.

    Science.gov (United States)

    Yang, R; Abraham, S; Gardner, G E; Ryan, U; Jacobson, C

    2017-05-01

    Develop a multiplex quantitative PCR assay to investigate the prevalence and shedding of Escherichia coli O157/O145, Salmonella spp. and Campylobacter spp. in sheep at sale yards and abattoirs. A qPCR for E. coli O157/O145 was developed, validated and multiplexed with an existing qPCR for Campylobacter and Salmonella enterica. The absolute numbers of E. coli O157/O145, Campylobacter and Salmonella in control samples was determined using droplet digital PCR. These were then used as the controls in the multiplex qPCR on a total of 474 sheep faecal samples collected from two saleyards over a 4-month period (April-July 2014) and 96 effluent samples from an abattoir. The mutiplex qPCR was specific with a sensitivity of 5 organisms/μL faecal DNA extract for Campylobacter, S. enterica and E. coli O157/O145. The overall prevalence of Campylobacter, S. enterica and E. coli O157/O145 in faecal samples was 5.7%, 3.6% and 8.4% and in effluent samples was 18.8%, 6.3% and 5.2%, respectively. The pathogen loads of Campylobacter, S. enterica and E. coli O157/O145 in faecal and effluent samples was also determined via mutiplex qPCR. The overall prevalences of Campylobacter, S. enterica and E. coli O157/O145 were generally low (<6%), but point prevalences ranged considerably in healthy sheep (up to 26% for E. coli O157/O145). Further work to determine risk factors for shedding of bacterial organisms in meat sheep in the pre-slaughter period (on-farm, sale yards and lairage at abattoirs) could further reduce the risk of contamination of meat products. © 2017 Australian Veterinary Association.

  10. REGULATION OF HISTAMINE-INDUCED AND UTP-INDUCED INCREASES IN INS(1,4,5)P-3, INS(1,3,4,5)P-4 AND CA2+ BY CYCLIC-AMP IN DDT1 MF-2 CELLS

    NARCIS (Netherlands)

    SIPMA, H; DUIN, M; HOITING, B; DENHERTOG, A; NELEMANS, A

    1995-01-01

    1 Stimulation of P-2U-purinoceptors with UTP or histamine H-1-receptors with histamine gave rise to the formation of inositol 1,4,5-trisphosphate (Ins(1,4,5)P-3) and inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P-4) in DDT1 MF-2 smooth muscle cells. 2 Stimulation of P-2U-purinoceptors or histamin

  11. Downregulation of the Genes Involved in Reprogramming (SOX2, c-MYC, miR-302, miR-145, and P21) in Gastric Adenocarcinoma.

    Science.gov (United States)

    Khalili, Mitra; Vasei, Mohammad; Khalili, Davood; Alimoghaddam, Kamran; Sadeghizadeh, Majid; Mowla, Seyed Javad

    2015-09-01

    Many cell signaling pathways essential for normal stem cell development are involved in cancer initiation and progression. In the present study, motivated by a possible contribution of reprogramming process in induction of cancer, we compared the expression level of main genes involved in iPS generation, i.e., miR-302, miR-145, SOX2, c-MYC, and P21, in a series of tumor and non-tumor tissues of stomach. A total number of 34 tumors and their matched non-tumor (as control) gastric surgical specimens were obtained. The expression of the candidate genes was evaluated by using real-time PCR and immunohistochemistry (IHC) techniques. Our data revealed a significant downregulation of miR-302b, P21, and miR-145 genes in intestinal and SOX2 gene in diffuse type of tumor samples. SOX2, but not the other genes, showed a significant downregulation in both proximal (cardia and fundus) and distal (body and antrum) sites of stomach. Based on receiver-operating characteristic (ROC) analyses, the highest total area under the curve (AUC) was found for SOX2 (AUC = 82 %, P c-MYC expression, while non-tumor samples represented an intense cytoplasmic staining. Despite the fact that some hESC-specific genes are upregulated in tumors, our data revealed a significant downregulation of all candidate genes, except for c-MYC, in tumor samples of stomach. Moreover, ROC data demonstrated that SOX2 gene expression index is a better potential biomarker of gastric cancer, compared to other tested genes. SOX2 expression has a good sensitivity and specificity to discriminate correctly between tumor/non-tumor and also high/low grades of tumor malignancy. It seems downregulation of miR-302b, miR-145, and P21 could contribute to gastric tumor initiation and progression.

  12. 复合晶体硫化物Sr1.145TiS3的高分辨电子显微术研究

    Institute of Scientific and Technical Information of China (English)

    杨改英

    1998-01-01

    @@ 与(PbS)-{1.12}VS-2,Ba-xFe-2S-4等三元金属硫化物一样[1,2],Sr-{1.145}TiS-3的晶体结构也很复杂[3].在四维超空间群的基础上使用粉末X射线数据和Rietveld分析方法确定了无公度复合晶体硫化物Sr-{1.145}TiS-3的晶体结构[4].它的基本结构是由空间群分别为R3m和P31c的TiS-3亚点阵和Sr亚点阵沿着晶体的c轴相互穿插形成的.以六角晶系来标定,其晶格常数为a-1=a-2=1.15108nm,c-1(TiS-3)=0.29909nm和c-2(Sr)=0.5226nm.用透射电子显微术研究其结构特性的报导还未见到.本文首次报导用透射电子显微术在原子尺度研究复合晶体硫化物Sr-{1.145}TiS-3晶体中Sr原子和S原子相互调制的结果.

  13. Apoptosis Induction of Human Prostate Carcinoma DU145 Cells by Diallyl Disulfide via Modulation of JNK and PI3K/AKT Signaling Pathways

    Directory of Open Access Journals (Sweden)

    Young Hyun Yoo

    2012-11-01

    Full Text Available Diallyl disulfide (DADS, a sulfur compound derived from garlic, has various biological properties, such as anticancer, antiangiogenic and anti-inflammatory effects. However, the mechanisms of action underlying the compound's anticancer activity have not been fully elucidated. In this study, the apoptotic effects of DADS were investigated in DU145 human prostate carcinoma cells. Our results showed that DADS markedly inhibited the growth of the DU145 cells by induction of apoptosis. Apoptosis was accompanied by modulation of Bcl-2 and inhibitor of apoptosis protein (IAP family proteins, depolarization of the mitochondrial membrane potential (MMP, ΔΨm and proteolytic activation of caspases. We also found that the expression of death-receptor 4 (DR4 and Fas ligand (FasL proteins was increased and that the level of intact Bid proteins was down-regulated by DADS. Moreover, treatment with DADS induced phosphorylation of mitogen-activated protein kinases (MAPKs, including extracellular-signal regulating kinase (ERK, p38 MAPK and c-Jun N-terminal kinase (JNK. A specific JNK inhibitor, SP600125, significantly blocked DADS-induced-apoptosis, whereas inhibitors of the ERK (PD98059 and p38 MAPK (SB203580 had no effect. The induction of apoptosis was also accompanied by inactivation of phosphatidylinositol 3-kinase (PI3K/Akt and the PI3K inhibitor LY29004 significantly increased DADS-induced cell death. These findings provide evidence demonstrating that the proapoptotic effect of DADS is mediated through the activation of JNK and the inhibition of the PI3K/Akt signaling pathway in DU145 cells.

  14. In Vitro Proteolytic Processing of the MD145 Norovirus ORF1 Nonstructural Polyprotein Yields Stable Precursors and Products Similar to Those Detected in Calicivirus-Infected Cells

    OpenAIRE

    Belliot, Gaël; Sosnovtsev, Stanislav V.; Mitra, Tanaji; Hammer, Carl; Garfield, Mark; Green, Kim Y.

    2003-01-01

    The MD145-12 strain (GII/4) is a member of the genus Norovirus in the Caliciviridae and was detected in a patient with acute gastroenteritis in a Maryland nursing home. The open reading frame 1 (ORF1) (encoding the nonstructural polyprotein) was cloned as a consensus sequence into various expression vectors, and a proteolytic cleavage map was determined. The virus-encoded cysteine proteinase mediated at least five cleavages (Q330/G331, Q696/G697, E875/G876, E1008/A1009, and E1189/G1190) in th...

  15. Ins(1,4,5)P{sub 3} facilitates ATP accumulation via phosphocreatine/creatine kinase in the endoplasmic reticulum extracted from MDCK cells

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Jing [Medical Research Center, School of Medicine, Fukuoka University, Fukuoka 814-0180 (Japan); Department of Dental Implantology, School of Stomatology, Tongji University, Shanghai 200072 (China); Ogata, Shigenori [Joint Laboratory for Frontier Medical Science, School of Medicine, Fukuoka University, Fukuoka 814-0180 (Japan); Segawa, Masaru [Central Laboratory for Pathology and Morphology, School of Medicine, Fukuoka University, Fukuoka 814-0180 (Japan); Usune, Sadaharu [Research Laboratory of Biodynamics, School of Medicine, Fukuoka University, Fukuoka 814-0180 (Japan); Zhao, Yumei [Department of Pediatric Dentistry, School of Dentistry of Shanghai Tongji University, Shanghai 200072 (China); Katsuragi, Takeshi, E-mail: katsurag@fukuoka-u.ac.jp [Medical Research Center, School of Medicine, Fukuoka University, Fukuoka 814-0180 (Japan)

    2010-07-02

    So far, the content and accumulation of ATP in isolated endoplasmic reticulum (ER) are little understood. First, we confirmed using electron microscopic and Western blotting techniques that the samples extracted from MDCK cells are endoplasmic reticulum (ER). The amounts of ATP in the extracted ER were measured from the filtrate after a spinning down of ultrafiltration spin column packed with ER. When the ER sample (5 {mu}g) after 3 days freezing was suspended in intracellular medium (ICM), 0.1% Triton X and ultrapure water (UPW), ATP amounts from the ER with UPW were the highest and over 10 times compared with that from the control with ICM, indicating that UPW is the most effective tool in destroying the ER membrane. After a 10-min-incubation with ICM containing phosphocreatine (PCr)/creatine kinase (CK) of the fresh ER. ATP amounts in the filtrate obtained by spinning down were not changed from that in the control (no PCr/CK). However, ATP amounts in the filtrate from the second spinning down of the ER (treated with PCr/CK) suspended in UPW became over 10-fold compared with the control. When 1 {mu}M inositol(1,4,5)trisphosphate (Ins(1,4,5)P{sub 3}) was added in the incubation medium (ICM with PCr/CK), ATP amounts from the filtrate after the second spinning down were further enhanced around three times. This enhancement was almost canceled by Ca{sup 2+}-removal from ICM and by adding thapsigargin, a Ca{sup 2+}-ATPase inhibitor, but not by 2-APB and heparin, Ins(1,4,5)P{sub 3} receptor antagonists. Administration of 500 {mu}M adenosine to the incubation medium (with PCr/CK) failed to enhance the accumulation of ATP in the ER. These findings suggest that the ER originally contains ATP and ATP accumulation in the ER is promoted by PCr/CK and Ins(1,4,5)P{sub 3}.

  16. Safety of spray-dried powder formulated Pseudomonas fluorescens strain CL145A exposure to subadult/adult unionid mussels during simulated open-water treatments

    Science.gov (United States)

    Luoma, James A.; Weber, Kerry L.; Waller, Diane L.; Wise, Jeremy K.; Mayer, Denise A.; Aloisi, Douglas B.

    2015-01-01

    The exposure effects of a commercially prepared spray dried powder (SDP) formulation ofPseudomonas fluorescens (strain CL145A) on the survival of seven species of unionid mussels endemic to the Great Lakes and Mississippi River basins was evaluated in this study. The study exposures were completed within replicated 350-liter test tanks contained within a mobile bioassay laboratory sited on the shores of the Black River near La Crosse, Wisconsin. The test tanks were supplied with flowing, filtered river water which was interrupted during the exposure period.

  17. Hyperfine structure and isotope shift of the D/sub 2/ line of /sup 118-145/Cs and some of their isomers

    CERN Document Server

    Thibault, C; De Saint-Simon, M; Duong, H T; Huber, G; Jacquinot, P; Juncar, P; Klapisch, Robert; Liberman, S; Pesnelle, A; Pillet, P; Pinard, J; Touchard, F; Vialle, J L

    1981-01-01

    High-resolution laser spectroscopy has been performed on /sup 118-145 /Cs and /sup 119m,121m,122m,130m,134m,135m,136m,138m/Cs. The Cs nuclei have been produced either by spallation of La or by fission of U by the 600 MeV proton beam, from the SC at CERN. The hyperfine structures, spins, and isotope shifts have been measured. The charge radii changes deduced from the isotope shifts exhibit shell effects at N=82, isomeric staggering for N=64,66,67 and a strong odd-even staggering for all isotopes with N<82. (24 refs).

  18. Comment on "Communication: Simple and accurate uniform electron gas correlation energy for the full range of densities" [J. Chem. Phys. 145, 021101 (2016)

    CERN Document Server

    Karasiev, Valentin V

    2016-01-01

    A simple expression for the uniform electron gas (UEG) correlation energy, recently presented in Ref. [J. Chem. Phys. 145, 021101 (2016)], deviates from the reference quantum Monte-Carlo (QMC) data at large r_s. We propose to define one of the parameters from a requirement to match the large-rs QMC data. Functional with the new parameter provides much better agreement with the QMC data at large r_s without deterioration of the functional quality at small and intermediate r_s.

  19. HYPERFINE STRUCTURE SPECTRA FOR 4f45d6K\\-9/2→(23229.991)o\\-9/2 TRANSITION IN 143,145Nd+%143,145Nd+ 4f45d6K\\-9/2→(23229.991)o\\-9/2 跃迁超精细结构光谱

    Institute of Scientific and Technical Information of China (English)

    马洪良; 陆福全

    2001-01-01

    利用共线快离子束-激光光谱学方法测量了143,145Nd+ 6K\\-9/2→(23229.991)o\\-9/2跃迁的超精细结构光谱,得到了上、下能级的超精细相互作用常数.%Hyperfine structure spectra for 4f45d 6K\\-9/2→(23229.991)o\\-9/2 transition in 143,145Nd+ were measured by using collinear fast-ion-beam laser spectroscopy, and the magnetic dipole and electric quadrupole coupling constants of the metastable levels and excited levels were determined.

  20. 犬瘟热病毒在MA-104、Marc-145、Vero3种细胞内增殖效果的比较%Comparison on Multiplication Effect of Canine Distemper Virus in MA-104, Marc-145, and Vero Cells

    Institute of Scientific and Technical Information of China (English)

    王朝蕾; 崔卓宇; 吕永钢; 胡江锋; 焦铁军; 樊军喜; 王根红; 崔瑞宁

    2012-01-01

    试验比较了MA-104、Marc-145、Vero3种猴肾细胞增殖犬瘟热病毒的敏感性,按常规方法将犬瘟热病毒(CDV—XN112株)分别接种于MA-104、Marc-145、Vero3种细胞,通过观察细胞病变,确定收毒时间,比较病毒滴度。结果表明,犬瘟热病毒可在MA-104、Marc-145、Vero3种细胞内增殖,并出现典型细胞病变,效价均达到10^4.5TCID50/0.1mL以上.而Vero细胞增殖犬瘟热病毒更为经济高效,是增殖该病毒的理想细胞。%After canine distemper virus (CDV-XN 112 strain) was inoculated into MA- 104, Marc- 145 and Vero cells, the sensitivity of three types of monkey kidney to CDV was compared by observing cell pathological effect, determining the time for harvesting virus and comparing virus titers. The results showed that three cells were suitable for CDV, with typical cell pathological effect, and the titers of virus harvest fluids were more than 104.5 TCID50/0. lmL, among them Vero cell was more cost-effective and ideal for proliferation CDV.